LOCUS       DASRQF010000001         2489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1734555, whole genome shotgun sequence.
ACCESSION   DASRQF010000001 DASRQF010000000
VERSION     DASRQF010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1734555"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VFD79_00005"
     CDS             <1..699
                     /locus_tag="VFD79_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZH92457.1"
                     /translation="MPHYIGANGFNGLHGRALPPAIGIKLSNKDLTVIAESGDGDTYG
                     EGGNHFIHTIRRNVNIAHFVHDNQIYGLTKGQGSPTTAMGQVTTLQYDGVKVEPVKPL
                     ALSLTMGAGFVARGFSGDIPQLVELMKEAILYKGYAHLDIFQPCVVWNKVNTFQWYKD
                     RVYKLSEDYDQTDFNAAYEKALEFGDKIPTGILYKVEKETYEDRFQFIKDGPPLVDAE
                     LNPFDAEKLMKDFY"
     gene            713..2386
                     /locus_tag="VFD79_00010"
     CDS             713..2386
                     /locus_tag="VFD79_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HZH92458.1"
                     /translation="MDYTILVGGAAGQGIETVVLLLEKTIKRSGYNVFTYKNYMSMVR
                     GGHNFMQVRFSDKPNSTFSTKVDMIFAMTAETVDFHIGNLKENGLVILDDELHKDQDF
                     MHLSLQKIAKGIGNPKVAPTIGFGAIGKYFGLDFEIARKALEETFKGKVLQLNMEALE
                     KGYGLTETVVPLERKEDTKIIMNGNQALALGAIAAGVKYYCGYPMTPSTSVMSYISEK
                     SKEMGIVVDQIEDEVGALNMALGASYAGTRAMTGSSGGGFALMVEALSLAGILEQPVV
                     VVNAQRPGPATGLSTRTEQADLRFVIHAGHGEFPKMVISLRNPQDAFYQTARAFNIAE
                     KYQIPVIIMTEEYLTDYMMTMDPLDFSKVTIERNIVEAIEDSKERYKRYKYTESGVSP
                     RLIPGKSKGVEILVDSHEHDEYGNIDESIETRINMMKKRMNKLEELKEELLEPDQLGV
                     ENPDILLVGFGATHGALLDAVTRLNEDGLSVGALVYGDIWPFPTKLINKLGKGAKKII
                     TVEQNMTGQLESLILEKTRLEIFDRILKYDGRPINGDEIISNLNTILEG"
BASE COUNT          813 a          420 c          619 g          637 t
ORIGIN      
        1 atgccccact acattggagc caatggcttc aatggacttc atggaagggc tttgccaccg
       61 gcgattggta tcaagctgtc aaacaaggat ttgacggtga tcgcggaatc tggagatggt
      121 gatacctatg gagaaggagg aaaccacttt atacacacca taagaagaaa tgttaatatt
      181 gcacattttg tgcatgacaa tcagatatat ggccttacca aggggcaggg ttcacctaca
      241 actgccatgg ggcaggtgac aaccctgcag tatgatggag tcaaggtaga acctgtaaag
      301 ccgctggcac tttctctcac tatgggagcg ggctttgtcg caagaggctt ttctggagat
      361 ataccccagc ttgtggaact tatgaaggaa gccatactct acaagggtta tgcccacctg
      421 gatatattcc aaccctgtgt tgtatggaac aaggtcaata catttcaatg gtacaaggat
      481 agagtttata agctctctga ggattatgac caaactgatt tcaatgccgc atatgaaaag
      541 gctttggagt ttggagacaa aattccaaca gggattcttt acaaggtgga gaaggaaacc
      601 tatgaggata ggttccagtt cataaaggat gggccaccat tggtggatgc tgaattaaac
      661 ccttttgatg ctgaaaaact aatgaaagac ttctattaaa ggtgggggaa aaatggacta
      721 tacgatattg gtcggtggag cagcaggaca gggaattgag actgtggtgc ttctccttga
      781 gaagaccata aagagaagcg ggtataatgt tttcacatac aagaattaca tgtccatggt
      841 aagaggtgga cacaacttca tgcaagtcag gttttctgac aagcccaatt caaccttctc
      901 aaccaaggtg gacatgattt ttgcaatgac agctgagact gttgatttcc acataggaaa
      961 cctgaaagag aatggcttgg ttattcttga tgatgagctt cacaaggacc aggattttat
     1021 gcacttgtct cttcagaaaa ttgccaaagg aattggaaat cccaaggttg caccaacaat
     1081 aggttttgga gcaattggaa agtattttgg cttggatttt gaaatagcca gaaaagccct
     1141 tgaggagaca tttaagggga aagtccttca gttgaacatg gaggcattgg aaaagggata
     1201 tggattgact gaaactgtag ttcccttgga aagaaaagaa gacacaaaaa tcataatgaa
     1261 tggaaaccag gctctagcct tgggtgctat tgctgcaggt gtcaagtatt actgtggtta
     1321 tccaatgaca ccttcaacca gtgttatgag ctatatatca gaaaaatcga aagagatggg
     1381 aattgtggtg gatcaaattg aggatgaggt aggtgctctc aacatggctt taggtgcaag
     1441 ttatgctgga accagggcca tgaccggttc atcaggtggt ggatttgccc tgatggttga
     1501 ggcattgagt ctggcgggga tactggaaca gcccgttgtg gtagtcaatg cacaaagacc
     1561 gggaccggca acaggtttgt caacaaggac tgaacaggca gatcttaggt ttgtaattca
     1621 tgccggacac ggagaatttc caaagatggt aatttctcta agaaatccac aggatgcatt
     1681 ctatcaaact gcaagagcct tcaatattgc tgaaaagtat cagattccag tgataataat
     1741 gactgaagag tatttgaccg actacatgat gactatggat ccattggatt tttccaaggt
     1801 taccatagaa agaaatattg tggaggcaat tgaggattca aaggaaaggt ataaaaggta
     1861 caaatatact gaatcagggg tgtctccaag attgatacca ggaaaatcaa agggtgttga
     1921 gatattggtt gatagccatg agcacgatga atatggaaat attgatgaat caatagaaac
     1981 aagaattaac atgatgaaga aaaggatgaa taaactggag gaacttaagg aagagttgct
     2041 ggaaccggat cagctaggtg ttgaaaatcc tgatatactc cttgttggat ttggagcaac
     2101 ccatggtgca ctattggatg cagtgacaag gcttaatgag gatggattat cagttggagc
     2161 ccttgtttat ggtgacatat ggcctttccc aacaaaactt atcaacaaac tgggtaaagg
     2221 tgcaaaaaaa ataattaccg tggagcagaa tatgacagga cagcttgaaa gtctgattct
     2281 tgaaaaaacc aggttggaaa tattcgacag gatactcaaa tacgatggaa gaccaataaa
     2341 cggtgatgaa atcatcagca atctcaatac aattcttgag ggttgattgt tacaccagat
     2401 aaaacatggt aaaatagttt caagaataat ttaagaggtg ttgaaaatgg atgacaatca
     2461 ttccagtcct agatacgaga aaattgcac
//
LOCUS       DASRQF010000002         2793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_868025, whole genome shotgun sequence.
ACCESSION   DASRQF010000002 DASRQF010000000
VERSION     DASRQF010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868025"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VFD79_00015"
     CDS             complement(<1..252)
                     /locus_tag="VFD79_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92459.1"
                     /translation="MKKTFWYPIVFMALLTAIFTFVLAFMDYSTAERVAANEEYELQT
                     KILYVFDIDYQDTSVEAVDQLFNEKISLEDDVYIYQEGDS"
     gene            complement(249..1232)
                     /locus_tag="VFD79_00020"
     CDS             complement(249..1232)
                     /locus_tag="VFD79_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HZH92460.1"
                     /translation="MFSDLVSKYFIKQKMMRTVIVSLIPLLLVATYYFGLRVLLLLFV
                     VGVFGIGTEYLYEKKNNKKISEAVLITCMLYTMTLPPSTPFWIAAVGIVFGVLFGKMV
                     FGGFGKNIFNPALVARAFVYVSFPEPLTIEWTNPSLGFPGGFATYLNEGIEMVSTATP
                     MLLYRDSGATLPFMDLLIGNVSGSIGETSAILVLLAGAYLIYKKVASWEIMAGCLVGF
                     TALSSILYFAGVPSVPNPLYGMVAGGFFFGTVFMATDPVSGTKTKEGKWIYGIIIGVV
                     TVIIRGFALFAGGVMFAILVGNTFSPIIDESVKAYKNYRKEKAEGKKEVTA"
     gene            1385..2401
                     /gene="namA"
                     /locus_tag="VFD79_00025"
     CDS             1385..2401
                     /gene="namA"
                     /locus_tag="VFD79_00025"
                     /EC_number="1.6.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF010047.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH dehydrogenase NamA"
                     /protein_id="HZH92461.1"
                     /translation="MKTFEKYSIHGLEVPNRIVMPPMCMYSSDHTGTVKEFHRMHYGA
                     RAIGGTGLIIQEATAVEPRGRISDRDLGLWSDDHIPGLKSLVQTVKGYGARAGIQLAH
                     AGRKSESASDIPVAPSALAFSEDYRTPKELGLDEIKEVVQSFKAAAGRALLAGYDLLE
                     IHAAHGYLIHEFLSPITNKREDMYGGSLENRTRFLREILLEVRKVWPENKPLIIRVSA
                     TDYLQGGIDGDEIVRIVDQVKDLIHMVHVSTGAIAPASMKLYPGYQVQYAERIKRECG
                     IPTIAVGLIKDLDQVEEILQNGRADLVALGREILRNPNWVLNNMHSGGIQARYPDQYF
                     RAYR"
     gene            complement(2458..>2793)
                     /locus_tag="VFD79_00030"
     CDS             complement(2458..>2793)
                     /locus_tag="VFD79_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZH92462.1"
                     /translation="KDGLAFEEAATNYSSCPSKEAGGALGEFGRGQMVPEFEEAAFNM
                     EIGAISEPVKTQFGYHIIKVDAKNEAAEPDFEEVKDQVQQQVLARKQEEKYQNKISGL
                     KVKYPVEIY"
BASE COUNT          785 a          661 c          616 g          731 t
ORIGIN      
        1 ggaatctccc tcctggtata tgtatacgtc atcttcaaga cttatcttct cattgaacag
       61 ctggtcaacc gcctctacac tagtgtcctg gtagtctatg tcaaagacgt acagaatctt
      121 tgtctgtagc tcgtactcct catttgcagc cactctctcg gctgtggagt agtccatgaa
      181 agccagaaca aaggtgaaaa tggcagtgag cagagccatg aaaactattg ggtaccagaa
      241 tgtctttttc atgctgtcac ctctttcttg ccttcagcct tttccttcct gtagttcttg
      301 tatgccttta cactctcgtc aataattggc gagaaggtgt tcccaaccag tattgcaaac
      361 attacgcctc ctgcaaagag ggcaaacccc cttattatga cggtcacaac accgattatt
      421 atcccgtaga tccacttgcc ttcctttgtc tttgttcccg aaacaggatc ggttgccata
      481 aataccgttc cgaaaaagaa gcctcctgcc accatcccat aaagtggatt tggcaccgaa
      541 gggacccctg caaaataaag gattgagctt aaggctgtaa atccaaccag acagcctgcc
      601 atgatctccc aagatgcaac cttcttgtag ataaggtaag cacctgcaag aagtaccagg
      661 attgcacttg tctctccgat tgatcctgac acgttcccta taagcaggtc cataaaggga
      721 agggtcgctc ccgagtccct gtatagaagc attggtgtag ccgttgaaac catttcgatt
      781 ccttcgttaa ggtaggttgc aaaacctccc gggaaaccca gtgaaggatt tgtccactcg
      841 attgtaagtg gctctgggaa tgacacatag acaaatgccc tggctaccaa ggcagggttg
      901 aatatgttct ttccgaagcc tccaaaaacc atcttaccga aaagaacacc aaaaacaatc
      961 cccactgccg caatccagaa tggtgttgac ggcggaaggg tcattgtata cagcatacag
     1021 gttatgagga ccgcctccga aatctttttg ttgttcttct tttcgtaaag gtattctgtt
     1081 cctataccga aaactccaac cacaaataac aataataata ccctaagtcc gaaataatat
     1141 gtagccacca gcagaagtgg aataagtgag actatgacag ttctcatcat tttctgcttg
     1201 ataaaatatt ttgacaccag atctgaaaac aaaagatcac ctccaagttg atataagtaa
     1261 taaattccat actatattaa taccacattt tggatgatta attcaatatt atttagatac
     1321 acaaaattat aaatgatatt attacatatt ttagtggtat aaatgaaaac gaggtgataa
     1381 aaggatgaaa acatttgaaa aatacagtat acatggcttg gaggtcccta acaggatagt
     1441 catgcccccc atgtgcatgt actccagtga ccacacagga actgtaaagg aattccatag
     1501 aatgcactat ggagcaaggg caattggcgg taccggccta ataattcagg aagctacggc
     1561 tgttgagcca aggggaagga tatccgacag ggatttggga ctttggtctg acgaccatat
     1621 cccgggtttg aagtccttgg tccaaacagt aaagggatat ggggcaaggg ctggaatcca
     1681 gcttgcccat gcaggcagga agtcggagtc agcctccgac atccctgttg ctccatcggc
     1741 cctggccttt tcagaggatt acagaacacc aaaggagctt ggtcttgatg aaatcaagga
     1801 agtggtccaa agcttcaagg ccgctgcagg aagagccctc cttgcaggct atgacctttt
     1861 ggaaatccac gcagcccacg gctacctaat tcacgagttc ctgtctccaa ttaccaacaa
     1921 gagggaggac atgtatgggg ggtcactgga aaacaggacc cgatttctaa gagaaatcct
     1981 ccttgaagtg agaaaggttt ggccagagaa caagcctcta ataataaggg tctctgcaac
     2041 agactacctt cagggtggaa ttgatggtga cgagattgta aggattgtgg accaggtgaa
     2101 ggaccttatc cacatggttc atgtgagcac cggtgcaatt gcacctgcca gtatgaagct
     2161 ctatcccggc taccaggtcc agtacgccga aaggataaaa agggagtgtg ggatacccac
     2221 aatagcagtt ggcctcataa aggatcttga ccaggttgag gaaatccttc agaatggtcg
     2281 tgcggacctt gttgcccttg gtcgtgagat cttaaggaat ccaaactggg tcctgaacaa
     2341 tatgcactct ggagggatcc aggctagata cccagaccag tatttcagag catatagata
     2401 aggaaatata aaacccacct gacggaataa gaatccgaaa ggtgggtttt tgctttatta
     2461 gtatatctca accgggtact tgaccttaag tcctgatatc ttgttctggt acttctcttc
     2521 ctgctttctt gcaagcacct gctgctgaac ctggtccttg acctcttcaa agtctggctc
     2581 cgctgcttcg ttctttgcat caaccttgat gatgtggtat ccgaactggg tctttacagg
     2641 ctctgatatt gcaccgatct ccatattgaa ggccgcttcc tcaaactcag gtaccatttg
     2701 gcctctgcca aattctccca aggctcctcc tgcctctttg gaaggacatg atgaatagtt
     2761 tgtcgctgct tcctcgaatg caagtccgtc ctt
//
LOCUS       DASRQF010000003        10677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_741861, whole genome shotgun sequence.
ACCESSION   DASRQF010000003 DASRQF010000000
VERSION     DASRQF010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 10677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10677
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741861"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VFD79_00035"
     CDS             <1..268
                     /locus_tag="VFD79_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583217.1"
                     /note="with CbiNQ forms the ABC transporter for cobalt
                     import; Clostridia have two adjacent copies of this gene;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HZH92463.1"
                     /translation="LVSHSMEDIARLVNRIFVMHKGKVALDGPTREVFSKTDELEEMG
                     LGIPQITKFMKAFKSRGNSVKDDILTIEEAKAEILNYLRSKKDA"
     gene            261..1061
                     /locus_tag="VFD79_00040"
     CDS             261..1061
                     /locus_tag="VFD79_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019034503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HZH92464.1"
                     /translation="MLKDITIGQYFPGTSIVHKLDPRVKIIIVALFIASLFFVTSFIP
                     YIFIVAFIGIVISIAKLPVKFIIKGLKPLMFIILITFAINLFMTKGEVLAELGPLVIT
                     KEGLRQSVFMALRLIFLITGTSLLTLTTSPIALTDGIERLLSPFSRIGLPAHELAMMM
                     TIALRFIPTLLEETDKIMKAQMARGADFESGNILSRAKNLVPLLVPLFINAFRRADEL
                     ATAMEARCYRGGDGRTRLNELRFVRRDYAVLTGMTIFFAAIISSRYIW"
     gene            1066..1803
                     /gene="truA"
                     /locus_tag="VFD79_00045"
     CDS             1066..1803
                     /gene="truA"
                     /locus_tag="VFD79_00045"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HZH92465.1"
                     /translation="MRNIKLIIEYDGTNYWGWQTQESFPSIQEEIEKALYRVTGEKTV
                     LYGSGRTDAKVHALGHVANFRTESSIPGERFRFALNIELPEDIRVISSQEVPMDFHAR
                     FSATHKRYVYRIYNGEPERPLLRNYTCHIKHPLDVELMREAAKYFIGTHDFRSFRGRR
                     TSIKSSIRTIYSIEIEKKDEIIEIALEGNSFLRNMVRIIAGTLIEVGAGQRCKEEIPE
                     MIQDRDRRSAGRTAPAQGLFLEKVYYR"
     gene            1921..2349
                     /gene="rplM"
                     /locus_tag="VFD79_00050"
     CDS             1921..2349
                     /gene="rplM"
                     /locus_tag="VFD79_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004825858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HZH92466.1"
                     /translation="MKSYMAKANEVERKWYIIDAEDKVLGRLATEIATILRGKNKPIY
                     TPHVDTGDFVIVINADKIKLTGKKLEQKNYTYHTGYPGGLRQVSYGELMEKNPEKIIE
                     LAVKGMLPKNSLGRQMYRKLKVYRGTEHGHQAQKPEVYEV"
     gene            2375..2767
                     /gene="rpsI"
                     /locus_tag="VFD79_00055"
     CDS             2375..2767
                     /gene="rpsI"
                     /locus_tag="VFD79_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HZH92467.1"
                     /translation="MAATQFLGTGRRKTSVARVRLVPGNGQVTINKRDIEDFFNYETL
                     RVIAKEPLTITETADKYDVFVNVHGGGFTGQAGAIRHGIARALLEADGELRPILKKAG
                     HLTRDSRMVERKKYGLKKARRSPQFSKR"
     gene            complement(2896..4023)
                     /locus_tag="VFD79_00060"
     CDS             complement(2896..4023)
                     /locus_tag="VFD79_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZH92468.1"
                     /translation="MQYREFSVNKLNVSLLGLGCMRFPVLENDNSKIDVDRSTSIVRY
                     ALEKGINYFDTAYPYHGGESEAFLGRALEGIRREDYHLATKSPVWLMENHDDFMKYLD
                     EQLVNLNTNYVDFYLFHALNKNSFEKIKSLEFERFVERAKEEGKIRYIGFSFHDELPI
                     FKEIIDHYPWEFCQIQLNYMDTEYQAGLAGLEYAKNRGIDVVVMEPLKGGKLAQSPED
                     VLQVWNRSQTSRTPVRWALKWLHGLQGISVVLSGMSSLDQLEENIGISSDDDVLSPED
                     MDIVNMAAQIYRSRTKVDCTGCKYCIPCPQNVAIPNIFQLYNDAHVYGSMEEASKSYS
                     SLVEKEEDSSRCVECGLCEEKCPQNLAIIQYLKDARDDLYS"
     gene            4180..5274
                     /gene="gcvT"
                     /locus_tag="VFD79_00065"
     CDS             4180..5274
                     /gene="gcvT"
                     /locus_tag="VFD79_00065"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004832697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HZH92469.1"
                     /translation="MEAKKTPLYDEHVKLGGNVVDYAGWYLPVQYEGLVPEHEAVRNA
                     VGLFDVSHMGEITIKGKDALAFVNYLMTNDISTIVDNQVIYTFMCHPTGGVVDDLLVY
                     RYDGEFFYLVVNAANVDKDHKWILDNKGDFQVEIENISDSVGEVAIQGPMAEKVLQKL
                     TDYDLSQVKFFHLKRDIEVAGVNCMVSRTGYTGEDGFEVYCDKDGIVTVWNAILEAGQ
                     DEGIKPTGLGCRDTLRFEASLPLYGHEMDDEITPLEAGFKYFVKLDKEADFIGKDALN
                     KMWSEGLERKLAGIELLDRGIAREGYEVYKDGEKIGHITTGYKSPTLGKVIGNALIKT
                     EFTKLGTEVDIKIRNKFAKAKVISKKFLKK"
     gene            5293..5676
                     /gene="gcvH"
                     /locus_tag="VFD79_00070"
     CDS             5293..5676
                     /gene="gcvH"
                     /locus_tag="VFD79_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HZH92470.1"
                     /translation="MKVAKGLFYTKDHEWVKVEGDEAFIGISDYAQHHLGDIVYVELP
                     DVDDEFEKGDDFAAVESVKAASDVFMPVGGKVVAINEELMDDPALLNQDAFENWLIKV
                     ELADKAEVEELMNDEDYEKFLAEEE"
     gene            5678..7021
                     /gene="gcvPA"
                     /locus_tag="VFD79_00075"
     CDS             5678..7021
                     /gene="gcvPA"
                     /locus_tag="VFD79_00075"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HZH92471.1"
                     /translation="MHPYIANTPEDIQEMLKAIGLESIDQLFDDIPKDIKLSRELDLP
                     KSKSELEVTSYLKKLADKNCSMSELTSFLGAGAYDHYIPSIVNHVISRSEFYTSYTPY
                     QPEISQGTLQYIFEFQTLIANLTGMDLANASLYDGGTAVVEAALMAAAYSRKDEIIIS
                     KSVKPDARKILHTYAHVQDLKVIEVDIKDGVTDMEALDGLVNDNTAAVIVQSPNFFGI
                     IEDLEAIGEIAHKGKKTTFIASVDPISLGILKKPGDMNVDVVVGEGQSLGIPVSFGGP
                     YLGFMAVKKDYVRKIPGRIVGQTEDLDGKRSFVLTLTAREQHIRREKATSNICSNQGL
                     NSLAATVYMVTMGKEGLREVALQSTKKAHYAYEQLIKSGKFKPAFDKPFFKEFALTSD
                     IAPEKINEALRKENIIGGYDLGKEYPQFENSVLYAVTEKRTKEEIDKLTSVMEGI"
     gene            7021..8475
                     /gene="gcvPB"
                     /locus_tag="VFD79_00080"
     CDS             7021..8475
                     /gene="gcvPB"
                     /locus_tag="VFD79_00080"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HZH92472.1"
                     /translation="MRNYEKLIFELSKPGRKAYMLPANDVEDVAVDSLIPGEFLSESE
                     LDLPELYEVDIIRHYTNLSNKNYGLDTGFYPLGSCTMKYNPKINEDTARYDGFANLHP
                     LQPDDCTQGSLQLLHELELSLSEIAGMDRTTLQPAAGAHGELTGVMIIKAYHEKRGEA
                     HRNKIIVPDSAHGTNPATAGMAGYKIVEIKSTRDGLVDLDALRAAVGDDTAGLMLTNP
                     NTLGIFDKGIKEIADIVHEAGGLLYYDGANANAILGYARPGDMGFDVVHFNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPVPMVEKKDDKYFLDYDYPDSIGKVKDFYGHFGILV
                     RAYTYILTMGSDGLKKASEMAVLNANYLCSQLKDYYHLPIDTIYKHEFVLGGLKDTLS
                     EVTTLDIAKRLLDLGYHPPTIYFPLIINEALMIEPTETESKETLDEFIQAMIQIAKEA
                     ESDPAILKSAPNRTKVRRPNEVKAARDINVKYEG"
     gene            8485..9900
                     /gene="lpdA"
                     /locus_tag="VFD79_00085"
     CDS             8485..9900
                     /gene="lpdA"
                     /locus_tag="VFD79_00085"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HZH92473.1"
                     /translation="MTKRIAIIGAGPGGYVAAIKAAQLEAEVFLIENRDIGGTCLNRG
                     CIPTKTYFRNAEIMSSLKRADEFGIRVEGYSLDGKALLERKNKVVDDLVSGIEKLISS
                     YSNIEFVPGKASFKDNKTLTVELLEGGTREIEADNIIIATGSYPQMTETKGVDLPGVI
                     TSDDILELDHIPETLIVVGGGVIGLEFASIYQELGSHVILLASRILKDGDKEIGKRLL
                     PMLKKQGMETYVNIRAKEISKEGDRLKVLAKYKEKDEEIEVVGDYVLIASGRGPLVEG
                     LGLEEAGVEYSNRGIQVDDNYETTVPGIYAIGDVNSKGIQLAHVASSQGEYVVEKIMG
                     HEPDLNHEHWPSCVFTMTEVAQVGYTEEQLKEREVDYRSSKFMFSANGKALSLGEPEG
                     IIKILASNEDNRILGVHILGPHANDLIHEGALAISNNMDVASIKRTIHAHPTLSEAFF
                     EATLGLDEAAIHIAPKRKRRG"
     gene            10150..10575
                     /locus_tag="VFD79_00090"
                     /pseudo
     CDS             10150..10575
                     /locus_tag="VFD79_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
BASE COUNT         3351 a         2042 c         2572 g         2712 t
ORIGIN      
        1 tctagtttcc cacagcatgg aggacatagc cagacttgtc aacaggatat ttgtaatgca
       61 caagggcaag gttgctcttg acggcccgac aagagaggtg ttctccaaga cagatgagct
      121 tgaggagatg ggccttggaa ttccacagat cacaaagttt atgaaggcct tcaagagcag
      181 gggtaatagt gtgaaggacg atatccttac cattgaagaa gccaaagcgg aaatattaaa
      241 ttatttaagg agtaagaagg atgcttaaag atattacaat cggacaatat tttccaggaa
      301 catctatagt tcacaaattg gatcctagag tgaaaataat cattgtagca ttgtttattg
      361 catctctctt ctttgtgaca agcttcatac catatatatt catcgtggcc ttcattggaa
      421 ttgtaatttc catcgcaaag ctaccggtta aatttatcat aaaaggtttg aagcccctga
      481 tgtttataat cttaataacc tttgccatca acctgttcat gaccaaagga gaggttttgg
      541 ctgagttggg ccccttggta ataacaaagg agggcttgag acagtctgtc ttcatggctc
      601 taaggctgat tttccttata acgggaacat cgcttttgac ccttaccaca tctccaatcg
      661 ccttgacaga cggaatagaa aggcttctgt ctccttttag cagaatcgga ctaccggcac
      721 acgagctggc catgatgatg acaatagccc taagatttat tccaacactt cttgaggaaa
      781 ctgacaagat aatgaaggct cagatggcaa gaggagcgga cttcgaatcg gggaatattt
      841 taagcagggc aaagaatctt gtaccccttc tcgttcccct gtttatcaat gccttcagaa
      901 gggcagatga gttggccact gccatggagg caaggtgcta ccgtggtgga gacggcagga
      961 caagacttaa cgagctaagg tttgttagga gagactatgc agtcctgacg ggaatgacaa
     1021 tcttttttgc agcgataata tccagccgat atatatggtg ataatatgag aaacatcaag
     1081 cttataatag aatacgacgg tacaaactac tggggatggc agacccagga gagctttccc
     1141 tccattcagg aggaaataga gaaagctctt tacagggtga ctggtgaaaa gactgtactg
     1201 tatggatcag gaaggacaga tgcaaaggtt catgcccttg ggcatgttgc taatttcagg
     1261 actgaatcat ccatacctgg agagagattc aggtttgcct tgaatattga gcttcctgaa
     1321 gacatcaggg tcatatcgtc ccaggaggta ccaatggatt ttcacgctag gttcagtgca
     1381 acacacaaga ggtatgtcta caggatatac aacggtgagc cagaaaggcc cctgcttagg
     1441 aattatacat gtcatatcaa acatccattg gacgtagaat tgatgagaga agcggccaag
     1501 tactttattg gaacccatga tttcagatct ttcagaggga ggagaacttc cataaaatca
     1561 tccatacgga ccatttattc aatagaaatt gaaaaaaaag acgagataat tgaaatagca
     1621 ctggagggaa acagctttct ccgaaatatg gtcaggatca ttgcgggaac ccttattgag
     1681 gtaggagccg gccagaggtg caaggaggag attcctgaaa tgattcagga cagggataga
     1741 cggtctgctg gaagaacagc accagcccag ggactatttt tggaaaaagt atactacagg
     1801 taaaatctgc cttgacattg attgttactt gtattagaat atataagagt gtttaaaaag
     1861 aagcccggaa actttttagt agacacctta aaacgaatgt tgttgtaagg agggaaatag
     1921 atgaaaagct atatggcaaa agccaatgaa gtcgaaagaa aatggtatat catagacgca
     1981 gaagataaag tattgggaag actggccaca gagattgcaa ctattctgag aggtaagaat
     2041 aagcccatct acacaccaca tgttgataca ggtgattttg ttatagttat aaacgctgac
     2101 aagatcaaac ttactggaaa gaaactggag cagaaaaact acacatacca tacaggttat
     2161 ccaggaggct tgagacaggt atcatacggc gagctgatgg agaagaatcc cgagaagata
     2221 attgagctgg ctgttaaagg aatgctgcca aagaatagtc tgggaagaca aatgtacagg
     2281 aaacttaagg tttacagagg tacagagcac ggacaccagg cgcagaagcc agaagtttac
     2341 gaagtttaat taattgatga agggaggact gtaaatggct gcaacacaat ttttaggaac
     2401 aggaagaaga aagacatccg ttgcaagagt tagattagta ccaggtaacg gacaggtcac
     2461 aatcaacaaa agagatattg aggacttttt caactacgag acactaagag taattgcaaa
     2521 agagcctttg accataactg aaacagcaga caagtacgac gtatttgtaa atgttcacgg
     2581 cggtggattc acaggacagg ccggagcaat cagacacggt attgccagag ctctacttga
     2641 agcagatgga gagttaagac caatacttaa gaaggcagga catcttacta gagattcaag
     2701 gatggtagag agaaagaaat acggtctaaa gaaggcaaga agaagtccac agttcagcaa
     2761 gagataatag gaaagcaccc attgggtgct tttttttagc cctttttaat tgttaacaaa
     2821 ggttatcccc tgtggattgt ggataatgtg gataaggtgg ataagtaccc gaacatgcgt
     2881 atcaggagct atttatcagc tgtataggtc gtctctggca tccttgagat actggattat
     2941 ggcaaggttc tgtggacact tttcctcaca cagtccacac tccacacatc tggagctgtc
     3001 ctcctccttt tcaacgaggc tggaatatga tttggaggcc tcctccatgg atccatatac
     3061 atgggcatcg ttgtaaagct gaaagatatt cgggattgca acattctgtg ggcaggggat
     3121 gcaatacttg cagcctgtac agtccacctt ggtcctgctc ctatatatct gggctgccat
     3181 attcacaatg tccatgtcct ccggtgatag aacatcatca tcagagctaa ttcctatatt
     3241 ttcctcaagc tggtccagag aggacattcc gcttaggaca actgatatgc cttgcagacc
     3301 atgaagccac ttcagagccc atctaaccgg cgtacgggag gtttgcgacc tattccacac
     3361 ctggaggaca tcctcaggtg attgagccag cttgcccccc ttaaggggct ccatgaccac
     3421 tacgtctata cctctgtttt ttgcatattc aagacccgct agaccagcct gatattcagt
     3481 gtccatgtag ttcaattgga tctgacagaa ctcccaggga taatgatcga ttatctcctt
     3541 aaaaatcggt agctcatcgt ggaatgaaaa tccgatatat ctgattttgc cttcctcctt
     3601 ggccctttct acgaaccttt caaattcaag agattttatt ttttcgaaag aatttttatt
     3661 aagggcgtga aaaaggtaaa aatccacgta gtttgtattt aaattaacca actgctcatc
     3721 aagatatttc ataaaatcat catgattttc cataagccaa acgggagatt tagtggccag
     3781 gtggtaatcc tcccttctga taccctcaag tgctcttccc aggaaagcct cagattcccc
     3841 tccgtgatag ggatatgcag tgtcaaaata attgattcct ttttcgaggg cataccttac
     3901 aattgaggtt gacctatcca catcgatttt tgaattatca ttctccaaaa caggaaaacg
     3961 catacaaccc aggcctaata gtgaaacatt taacttgttg acagaaaatt cacggtattg
     4021 cataaaaaat cccccttatg tataattgaa gtatactact gaaatacgac ttacttggta
     4081 taattcatgt aatccagatt agtttaactg ccaagcaagt gggtagttat aggatagtat
     4141 attgaaatta taccacaaat aagaggaagg agtgattgaa tggaagcaaa aaagacaccc
     4201 ctgtatgatg agcatgtcaa gttggggggt aatgttgttg actatgctgg atggtatctt
     4261 ccagtgcagt atgagggcct ggtaccagag catgaggctg taagaaatgc agttgggctt
     4321 tttgatgtat cgcacatggg agagataacg atcaaaggga aggacgcact tgcctttgtt
     4381 aattatctaa tgaccaatga cataagtaca atagtcgaca accaagttat ttacacattc
     4441 atgtgccacc caacaggagg ggtcgtggat gatttgcttg tgtacagata tgatggggaa
     4501 ttcttctatc tggtagttaa tgctgcaaat gttgacaaag accataagtg gattctcgac
     4561 aacaagggag atttccaggt cgaaatcgaa aacatttcag actcagttgg tgaggttgcc
     4621 atacagggac caatggctga aaaggtacta cagaagctta cagactatga cttgtcacag
     4681 gtcaagttct tccaccttaa gagggatatt gaggttgctg gagtgaactg catggtttca
     4741 agaaccggct acacaggtga ggacggtttt gaggtttatt gtgacaaaga tggtattgtt
     4801 acagtctgga atgcaatcct tgaggctgga caggatgagg gaatcaaacc tacaggatta
     4861 ggctgcaggg acacattgag atttgaggca tcacttccac tttacggaca tgagatggac
     4921 gatgaaatca ctcctcttga agcgggattc aagtattttg ttaagcttga caaggaagca
     4981 gacttcatag gcaaggatgc tctaaacaag atgtggagtg aaggccttga aaggaagctt
     5041 gctggaatag agcttcttga caggggtatt gccagagagg gctacgaggt ttacaaggat
     5101 ggcgaaaaga ttggccatat tacaacaggc tacaagagtc ccacacttgg caaggtaatt
     5161 ggtaatgcac ttatcaagac agagttcaca aagcttggaa cagaggtgga catcaagatc
     5221 agaaacaaat ttgcaaaagc aaaggttata agcaagaaat ttcttaaaaa ataaattcta
     5281 gggggtaatc aaatgaaagt agcaaaagga ttattttaca caaaggatca tgagtgggta
     5341 aaggtagagg gagacgaggc atttataggt atttctgact atgcacagca tcatcttggg
     5401 gacatagtat atgttgagct tcctgatgtt gacgacgaat ttgaaaaagg cgatgacttc
     5461 gcagcggttg aatcagttaa ggctgcctct gatgtgttta tgccagttgg cggaaaggta
     5521 gtagcaatca atgaagagct tatggatgat cctgcactac ttaaccagga tgcatttgaa
     5581 aactggttga taaaggttga attggcagac aaggctgaag tagaagaact aatgaatgat
     5641 gaggactacg aaaagttttt agctgaggag gaataagatg catccatata tcgctaatac
     5701 acctgaagat atccaggaaa tgctcaaggc gattggtctg gaaagcatag accagttgtt
     5761 tgatgatatt ccaaaggata ttaagctttc aagagagctt gatcttccaa aatcgaaaag
     5821 tgaacttgaa gtaacctcat atctgaagaa gcttgctgat aagaactgtt caatgagtga
     5881 gctgacaagc tttttgggag caggtgcgta cgatcactat ataccatcaa tagtaaatca
     5941 tgttatatca agaagtgagt tctacacttc ttatacgcct taccagcctg aaataagcca
     6001 gggaacattg cagtacattt tcgagttcca gaccctgatt gcaaatctta caggaatgga
     6061 tcttgccaat gcatcacttt atgatggtgg tacggcagta gttgaggcag cccttatggc
     6121 agctgcctac tcaagaaaag acgagattat tatatcaaag tcagttaaac ctgatgcaag
     6181 aaagattctt cacacatatg cgcatgttca ggatctgaag gtaatcgagg tagatatcaa
     6241 ggatggagtt actgacatgg aagcccttga tggactggta aatgacaata ctgctgcagt
     6301 tattgtacaa agtcctaact tcttcggaat aatcgaggac cttgaagcaa ttggagaaat
     6361 tgcccacaag ggtaagaaga ctacctttat agcttccgtt gatccgattt cacttggtat
     6421 tctaaagaag cctggtgaca tgaatgttga tgttgtagtc ggagaaggac agtcactggg
     6481 aataccagtt agctttggtg gaccttatct tggattcatg gcagtcaaga aggactatgt
     6541 gagaaagatt cctggaagga tagttggaca gaccgaggac cttgacggca agagatcctt
     6601 tgtgctgacc cttacagcca gagagcagca tataagaaga gagaaggcaa catcaaacat
     6661 ctgctcaaac caggggctta attcactggc agcaactgtc tacatggtga caatgggcaa
     6721 ggaaggcctt agggaggttg cattgcaatc aaccaagaag gctcactacg cctatgagca
     6781 gcttattaaa tcaggcaagt tcaagcctgc atttgacaag ccattcttca aggaatttgc
     6841 attgacttcc gatattgcac ctgaaaagat caatgaagcc cttagaaaag aaaatatcat
     6901 cggaggctat gacttaggca aagaatatcc ccagtttgaa aatagcgtcc tttatgcagt
     6961 gacagaaaag agaacgaagg aagaaatcga taaactgacc agcgtaatgg aggggatata
     7021 atgagaaact acgagaaatt gatattcgaa ctttccaaac ctggtagaaa ggcatacatg
     7081 ttacctgcaa atgacgtgga ggatgttgct gtagacagcc ttatacctgg ggaattctta
     7141 agtgagtccg agttggacct tccagaattg tacgaggtgg acataattcg tcattataca
     7201 aatctatcaa ataagaatta tggccttgac acaggattct acccacttgg atcatgtaca
     7261 atgaaataca atccaaaaat caatgaagat acagcaagat acgacggatt tgcaaatcta
     7321 catcccttgc agccagatga ctgcacacag ggatcacttc agcttctaca cgagcttgag
     7381 cttagtctgt cagaaattgc cggaatggat agaacaactc ttcaaccagc agcaggagcc
     7441 catggcgagc tgactggagt aatgataata aaggcttacc acgaaaagag aggggaagca
     7501 cacagaaaca agataatagt tcctgattca gcacacggta caaacccagc cacagcagga
     7561 atggcaggat acaagattgt ggaaataaaa tcaacaagag atggactggt tgacctggat
     7621 gcacttcgtg cagctgtggg agatgacact gcaggcctta tgcttacaaa tcccaatacc
     7681 cttggaatat ttgacaaggg aatcaaggaa atagcggata ttgtccacga ggcaggggga
     7741 cttctctatt acgatggagc caatgccaat gctatcctgg gctatgcaag acctggagac
     7801 atgggatttg acgtcgttca cttcaacctg cacaagacct tctcaacacc acatggtgga
     7861 ggaggaccag gagcaggacc tgtaggagta aagaaggagc ttgttccatt cctgcctgtt
     7921 ccaatggtgg agaaaaagga tgacaagtac ttccttgatt atgactatcc cgattcaatc
     7981 ggaaaggtta aggatttcta cggacacttc ggtatccttg taagagccta cacctatata
     8041 ttgacaatgg gttcagatgg actgaagaag gcttcagaga tggcagtctt gaatgcaaac
     8101 tacctctgca gtcagttgaa ggattactac caccttccaa tcgacaccat ctacaagcat
     8161 gagtttgtac tgggaggatt gaaggacaca ttaagtgagg ttaccactct tgatattgcc
     8221 aagagactgc ttgaccttgg ataccatcca ccaacaatct acttcccatt gattatcaac
     8281 gaggcactta tgattgagcc gactgaaaca gaaagcaagg agaccctgga tgagtttatc
     8341 caggctatga tccagattgc caaggaggcg gaatccgacc ctgcaatcct taagtcagct
     8401 ccaaacagaa caaaggtaag aagaccaaat gaagtcaagg cagcacgaga catcaacgta
     8461 aaatacgaag ggtaggtgta ttgcttgaca aagagaatag ctattatcgg tgctggaccg
     8521 ggtggctatg ttgcagccat aaaggctgcg caacttgagg cagaggtttt tcttatagaa
     8581 aacagagaca ttgggggtac atgccttaac agggggtgta tccccactaa aacatatttc
     8641 aggaatgctg aaataatgtc ctcactcaag agagccgatg agtttggtat aagggtggaa
     8701 ggctacagcc ttgatggaaa ggctcttctg gagagaaaga acaaggttgt ggatgacctt
     8761 gtgtccggaa tagaaaagct tatttcatcc tacagcaata tcgaattcgt accgggtaag
     8821 gcatctttca aagataataa aacattgaca gtagagcttc tggaaggggg aacccgagaa
     8881 atagaagctg acaatatcat tattgcaaca gggtcctacc cccagatgac cgaaacaaag
     8941 ggtgtagacc ttcctggagt catcacatct gacgatatcc tggagctgga ccacatccca
     9001 gagacactta tagtagtggg tggaggagtt attggacttg agtttgcaag catatatcag
     9061 gagctcggtt cacatgttat acttctggca tccaggatcc taaaggatgg ggacaaggaa
     9121 atcgggaaaa ggttgctacc gatgcttaag aagcaaggta tggagaccta tgtgaatatc
     9181 cgtgcaaagg aaatctccaa ggaaggggac aggctgaagg ttcttgccaa gtacaaggaa
     9241 aaggacgagg aaatagaggt agttggagac tatgtcctga ttgcgtcagg aagagggcct
     9301 cttgttgagg gacttggcct tgaagaagca ggggttgagt actcaaatag gggcatacag
     9361 gttgatgata actacgagac tacagttcct ggaatctatg caattggaga tgtaaactca
     9421 aaggggatcc aattggcaca cgttgcttca tcccagggtg agtacgttgt tgagaaaata
     9481 atgggtcatg agcccgactt gaatcacgag cattggccat cctgtgtgtt tacaatgaca
     9541 gaggttgctc aggtgggcta cactgaagag cagcttaagg agagggaagt ggactacagg
     9601 tccagcaagt ttatgttctc agccaacggt aaggccctgt cattgggaga acctgaaggg
     9661 ataatcaaga ttcttgcttc aaatgaggat aacaggattc ttggtgttca cattctggga
     9721 ccacacgcaa atgaccttat ccacgagggt gccttggcaa tttccaacaa catggatgtg
     9781 gcttcaatca aaaggacaat acatgcccat ccaacacttt cagaggcatt ctttgaggcg
     9841 acactgggac ttgatgaggc tgcaatccac attgcaccca agagaaaaag aagaggatag
     9901 caagagcaac ggggagaccc gttgcttgat tttttcaggg ggacttgtat gacaacaaag
     9961 aaaaaactat tgattgttat ttgctccatt gtatttttaa tcataggatt tatggtaatg
    10021 ggtaagtctg aaggtgtact atttgaccag gtcatcatgg attctgtaca caggaataat
    10081 aacattattt tgatcagagc catggaattg atatcatttc ttggatcgga ggcattcctg
    10141 tttccggcca tgggcactgc aatagcctac tacaccataa agaagaaata tcttgtagca
    10201 ggactcctac tgacctcttc attgggcagc tggctgatga accaccttct taagcagata
    10261 tttcagaggc caagaccctt tgaatatttt ctggtagacc agggaggtct tagctatcca
    10321 agtggacact caatggtatc catgaccatg ttcctgacaa ttgcctacct ccttacaaga
    10381 aattccacca ggggaggaag gaataaatgg atttgatctg gggcacttgc ctatgttcta
    10441 ctcatgggga ccagtagggt ctttcttggt gttcattggc ccactgacat aattggagga
    10501 tttgctgcag gctatctttt ctatgaaggg tacatgaata ttatcagggg taagataaaa
    10561 agggattccg tctaatcgga atcccttttt tcctaattga tgctttcatc gccaaggtac
    10621 tggtttgtgt agagtctatg gtaatatcct tttttcatca gcaattctct gtgggtt
//
LOCUS       DASRQF010000004         6343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1117068, whole genome shotgun sequence.
ACCESSION   DASRQF010000004 DASRQF010000000
VERSION     DASRQF010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6343
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117068"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VFD79_00095"
     CDS             <1..300
                     /locus_tag="VFD79_00095"
                     /inference="COORDINATES: protein motif:HMM:NF025099.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA 5TM domain-containing
                     protein"
                     /protein_id="HZH92474.1"
                     /translation="LAFRGYQLVWPIFGSANQLLAALALLALALWLKRTGRQYKMAVY
                     PMVFMFIATLVALVLLMMANLANPILLVFAVALFILAIVLILESRAAFASTEELD"
     gene            364..651
                     /locus_tag="VFD79_00100"
     CDS             364..651
                     /locus_tag="VFD79_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92475.1"
                     /translation="MSRLKKFITDFGLFFKGATDFKGRAYEMLESETKTEMDNFILIC
                     FADQLGMPLPISYYTLELLPYLVDEIEDWDKRMGKRKSVWEERWSNYDLDP"
     gene            669..1580
                     /locus_tag="VFD79_00105"
     CDS             669..1580
                     /locus_tag="VFD79_00105"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HZH92476.1"
                     /translation="MAKIIFYGGKGGVGKTTCSTANSINYAEKGLKTLLVSTDPAHSI
                     SDVLGMDIGRSIVRIRENLDGLEIDPEYEAELYIEKIRANMKTILSNVIVEEINSQLD
                     AAMVSPGTHESALFDKISDIIIDKYDDYDIIIFDTAPTGHTLRILTLPDLLEGWMGSL
                     AKKRKSIVNMNKMVTRKYDEKDPVLEILERRQKRFGEIKKIFNNTNNVRIRFVLNAEK
                     LPIEETKKALKMLKQFDIPVDTLVINRIMPDDIGAEFWEKKRKQEQEYLKEIEMEFQG
                     KEFIKIPLLDSDMNKSTVDMLSKYFGE"
     misc_binding    1658..1887
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1946..2863
                     /gene="cysK"
                     /locus_tag="VFD79_00110"
     CDS             1946..2863
                     /gene="cysK"
                     /locus_tag="VFD79_00110"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004838820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HZH92477.1"
                     /translation="MMYNSVLELIGNTPMVKFQGGEKGTADIYLKIERSNPGGSIKDR
                     AALFMIENAEKNGKLKAGDTIIEATSGNTGVALAMIGAIKGYRVIIVMPDTMSVERQR
                     LMSAYGADLILTPGKQGMSGSVEKAKALTIDNGYYMPGQFINKANVEAHYRTTAEEIW
                     RDMEGRVDVFVAGVGTGGTISGVGKRLKELNPEISIVAVQPEKSPVLTGGEPSSHGIQ
                     GIGANFIPQIYDHEVVDQVISMREEDAREKTLQLARQSGILAGISSGSNLAAAIKVAE
                     SLSPGKRVVTVLPDTGERYLSTDIFKKVE"
     gene            2864..3406
                     /gene="epsC"
                     /locus_tag="VFD79_00115"
     CDS             2864..3406
                     /gene="epsC"
                     /locus_tag="VFD79_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HZH92478.1"
                     /translation="MIRWIIEEGKNIMIKDPAAKSLLEAILFSPGLKAMAYHRLAHRQ
                     YRAGRLFLARFISQRARRITGIEIHPGAIIGNRLFIDHGMGIVIGETAEIEDDVTIYH
                     GVTLGGIGGDKGEKRHPTVKSGTIIGAGAKILGPITIGSNVKIGANSVVLKDVPDRYT
                     AVGAPARLIPEKKNNHIRIV"
     gene            complement(3448..4476)
                     /locus_tag="VFD79_00120"
     CDS             complement(3448..4476)
                     /locus_tag="VFD79_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HZH92479.1"
                     /translation="MNSKSIHQYIGKGPKNTITDVPGVRVGHCSLKEESIKTGATAII
                     PADGNIFKEKLIASAHVINGFGKSAGILQVQELGTLETPILLTNTLSVGAGLTALTRH
                     MLEGNEDIGVATGTVNSVVCECNDGKINSIRSMAVKENHFQQALKSASEDFQQGDVGA
                     GTGMVCYGLKGGIGSSSRRIQIVDEVYNLGILVLSNFGSMEDLLYYGAPVGKVISDRI
                     HNGSSVDKGSIIVILATDIPLDSRQLNRVLKRTQSGIGRTGGYTGNGSGEIALGFTTT
                     NRISHYPEMPVMDIQTLHEDYLDLVFLATVQATEEAILNSLIHSEPSKGIDGKKVFSL
                     KEFNELLK"
     gene            4573..6240
                     /locus_tag="VFD79_00125"
     CDS             4573..6240
                     /locus_tag="VFD79_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HZH92480.1"
                     /translation="MNYSILIGGAAGQGMDTLAQLLTKTLKRYGYFVFKHSDYMSRVR
                     GGHNFIQIRFSDKQVRTYEDKVDIIYALNKETIDLHSERLNEGGIVLVDKAVSDDENL
                     LLLDLEETAKEAGNKRAMTSVGMGAIIKLLGLEPDVALNVIGSTLKDSIVDVNKEAFS
                     KGYEMVGSRFGLKQEKDEKILIDGNTAVAMGAAAAGCRYYCGYPMTPSTTVLSNMEKW
                     SQELGIFTEQVEDEIAALNMALGASYAGVRAMTGSSGGGIALMSEALSLAGIIETPVV
                     IINVQRPGPATGLPTRTEQADLRFAIHSGHGEFPKMVISLRSPEDAFYQTARAFNIAE
                     KYQIPVILLSDQYLADGELTIEPFDFSKIEIERNLYEDELPAGRYKRYELTDTGISPR
                     IIPGKVEDKLILVDSDEHDEYGNITESAQMRTSMVDKRSKKGELLEGEVEEPWLIGDE
                     NPDNLLVCWGSLFGQVNEVVEILQEEGHSVGALVYGDIYPFPTRLLKENAATAKSIID
                     IENNSAAQLDGLMREFALIKSDHKILKYDGRAFSVDELYNRLKEVVE"
BASE COUNT         1994 a         1131 c         1524 g         1694 t
ORIGIN      
        1 ttggcattta ggggatacca gcttgtatgg ccaatatttg gatctgccaa ccagctgttg
       61 gcagcgcttg cccttttggc tcttgccctc tggttgaaga gaacaggaag acaatacaaa
      121 atggcagttt acccaatggt attcatgttt atagcaacct tagtggccct ggtcctattg
      181 atgatggcaa accttgcaaa tccaatactc ttggtatttg cggttgcatt gttcatcctt
      241 gcaattgttt tgatactgga atccagagca gcctttgcaa gtacagagga attagactga
      301 tggcaatctg aaaagtataa gagaggagcc taggcttctc tcttttatat aaggggattt
      361 attatgagta gactaaagaa gtttatcaca gattttggac tgttctttaa aggagctaca
      421 gattttaaag gcagggccta tgaaatgctg gaaagtgaaa caaagactga aatggataac
      481 tttatcctca tctgttttgc cgatcagctt ggaatgcccc ttccaattag ctactacaca
      541 ttggagctgc ttccatactt ggtggatgaa atagaggatt gggacaagcg tatgggaaaa
      601 cggaagagtg tatgggaaga acgatggtca aattatgacc tggatcccta ggatgacgga
      661 ggaagaaaat ggctaagatt attttttacg gagggaaagg tggtgtcggc aagacaacct
      721 gctccactgc caacagtatt aattatgcgg aaaaaggttt aaagactctc ctggtatcaa
      781 ccgaccctgc ccattcaatt tctgatgttc tgggaatgga tatcggaagg agcatagtca
      841 ggataaggga gaatctggat ggacttgaaa ttgatcctga atatgaggcg gagctttata
      901 ttgaaaaaat aagggctaac atgaaaacaa tattgtcaaa tgtaatcgtc gaggaaatca
      961 atagtcagct tgatgcagcc atggtatcac ctggtaccca cgaatcagca ttgtttgaca
     1021 agatttccga cataataata gacaaatatg atgattacga tattataata tttgacacag
     1081 ctccaactgg tcataccttg aggattctaa cacttcctga tcttttggaa ggctggatgg
     1141 ggtctcttgc caagaagaga aaatccattg tgaacatgaa caaaatggtc acaaggaagt
     1201 atgatgaaaa ggatccggtg cttgaaatcc ttgaacgtcg tcagaaaaga ttcggagaaa
     1261 tcaagaagat attcaacaat accaacaacg ttaggatccg atttgtgcta aatgctgaaa
     1321 aattaccaat agaggagaca aaaaaggctt taaaaatgct taagcagttt gatattcccg
     1381 ttgatactct cgtaataaac aggattatgc cagatgatat tggggctgaa ttctgggaga
     1441 agaaaaggaa acaggaacag gaatatctaa aggagattga aatggaattt caaggcaagg
     1501 aattcataaa gattcccttg ttggattcag atatgaataa gtcaacggtt gacatgctat
     1561 caaaatactt tggagagtaa ttattttagt ttttgatatt ttagacttgc aattttcata
     1621 agtcatgtta taataatcaa gtaaatataa atacaactga tttgctagga attggtaaag
     1681 tacattttga tttgtattcc agagaggctg ttgttggtgg gaaacagtta tgcaagcatt
     1741 gtggaagtgc gccaaggagc atttgaccga aggtaatgta ggtcaacacg ggagttcccg
     1801 ttacagaact agggtatgac tgtgcccgaa gagatgggga aatcccaaac agagtggaac
     1861 cgcgaagata taacctttgc ctctgtataa ttaagtttat actgaggcag aggttttttg
     1921 ttttttaatt ttaaggaggt tgaatatgat gtacaatagt gtattggaat tgattggaaa
     1981 tacaccaatg gttaaatttc aaggaggaga gaaagggact gccgatatct atttgaagat
     2041 tgaaaggtcc aatcctggag ggagcattaa ggaccgcgct gcccttttta tgattgaaaa
     2101 tgctgaaaag aatggaaaac taaaggcagg agatacaatc atagaagcta caagcgggaa
     2161 cacaggagtg gcacttgcaa tgataggagc aatcaaggga tacagggtta tcattgtaat
     2221 gccggataca atgagtgttg aaagacagag attgatgtct gcctatggag cggatttgat
     2281 tctgacacca ggtaagcaag gtatgagtgg ctcggtagaa aaggccaaag ccttgaccat
     2341 tgataatggg tattatatgc cgggtcagtt tataaacaag gccaatgttg aagcacacta
     2401 tagaactact gcagaggaga tatggagaga tatggaaggt agagtggacg tctttgttgc
     2461 aggggttgga accggtggaa caatcagtgg agtaggaaaa cgtcttaagg agctgaatcc
     2521 agaaataagc attgttgcag ttcagcctga aaagtctcct gtactcactg gtggggagcc
     2581 atccagtcat ggtattcagg gaattggagc aaatttcatc ccccaaattt acgaccatga
     2641 agtggtggac caggttataa gcatgaggga agaggatgct agagaaaaaa ctctacagct
     2701 tgcaagacaa tcagggattc tagcaggaat aagctcagga tcgaatctgg ccgctgcaat
     2761 taaagttgca gaaagcctta gtccaggtaa aagggtggtt actgtcctgc ctgatacagg
     2821 tgaaagatat ttatctacgg atatattcaa gaaggtggaa taaatgatca gatggattat
     2881 tgaagaaggt aagaacataa tgataaaaga cccagcagcc aagtccctac ttgaagccat
     2941 actgttttca ccaggactga aggccatggc atatcacagg ctagcccaca gacaatatag
     3001 ggcaggaagg ttatttttag ccaggtttat ttcacagagg gctagaagaa taacaggaat
     3061 cgaaatccat ccaggtgcaa taatcggaaa tagactattt attgatcatg gcatggggat
     3121 tgtaattgga gaaacagcgg aaattgaaga tgatgtaaca atctatcatg gcgtcacctt
     3181 gggaggcatc gggggagata aaggtgaaaa aagacatcct actgtaaagt ctggtactat
     3241 tatcggtgca ggagcaaaga ttctaggtcc cataaccatc ggaagcaatg tcaagatagg
     3301 agcaaactct gttgttttaa aggatgttcc agatcgttat actgccgtag gcgctcctgc
     3361 caggttgatc cctgaaaaga agaacaatca catcaggata gtatagaaaa tctccatcca
     3421 gattatgttc tttggatgga gatttttcta tttcaacaat tcattgaact cctttaggct
     3481 gaaaaccttc ttgccatcaa taccttttga aggttcagaa tgaatcagag aattcagtat
     3541 ggcttcctca gtagcttgga cagtggccag gaacacaaga tccaaataat cctcgtgtag
     3601 tgtttgaata tccatgacag gcatctctgg atagtgactg attctgttgg tggttgtaaa
     3661 gcccagggca atctcaccac tgccatttcc ggtataacct ccagtcctcc ctatacctga
     3721 ttgagttctt ttcaataccc tgttcagctg tcttgaatcc agtggaatgt ctgtggccag
     3781 gattactatg attgaaccct tatcaactga actcccatta tgaattcggt cgcttataac
     3841 ctttccaact ggtgctccgt agtaaagcaa atcttccatg gagccaaagt tagagagtac
     3901 aagtattccc aaattgtaaa cttcatcaac tatttgaatt cttcgtgagg atgagcctat
     3961 tccgcccttt aaaccatagc aaaccattcc ggttcctgct ccaacgtctc cctgttggaa
     4021 atcttccgat gcacttttga gggcctgctg aaagtgattt tccttaacag ccattgatct
     4081 gatactgttg atctttccat cgttacattc gcacactaca gaatttacag taccagtggc
     4141 taccccaata tcctcattgc cttcaagcat atgcctggta agagctgtta ggccagcacc
     4201 tactgagagg gtgtttgtca acagtatagg agtctcaagt gtgccaagtt cctgaacctg
     4261 aaggattcct gctgatttgc caaatccatt gataacatgt gcagaagcaa tcaatttttc
     4321 cttgaaaatg ttaccatctg caggaataat cgcagtagcc ccggttttaa tgctttcttc
     4381 ctttaagctg caatgcccca cccggacacc aggaacatca gttatggtat ttttgggccc
     4441 ttttcctatg tattggtgga tgcttttact attcattcaa tcagctcctt atagacttat
     4501 tcattgggat aattcttcat taagagggta tatactaatc atatgatata gccaaaaggg
     4561 gggagatgca agatgaatta ttcaatattg ataggtggtg ctgctggtca gggaatggac
     4621 accttggctc agctgcttac aaaaacatta aaaagatacg ggtattttgt tttcaagcat
     4681 agtgattaca tgtccagggt cagaggaggc cataacttca tccagataag gttctctgac
     4741 aagcaggtcc gtacatacga agacaaggtt gatataattt atgccttgaa caaggagacc
     4801 atagacttac acagtgagcg gttaaatgaa ggtggcattg ttcttgtgga caaggctgta
     4861 agcgatgatg aaaacctact tttacttgac ctggaggaaa ccgcaaagga ggctggtaat
     4921 aaaagagcca tgacaagcgt cggtatggga gcgattatca agctgcttgg acttgaacct
     4981 gatgtggcac tgaatgtgat aggatcaacc ttaaaagata gcatagttga tgtgaacaag
     5041 gaagcattta gcaagggcta tgaaatggtc ggatccagat tcggtttaaa acaggagaag
     5101 gacgagaaga tactaattga tggtaataca gctgtagcaa tgggagcagc agctgcaggc
     5161 tgcagatatt actgtggcta tcctatgacc ccctcaacca ctgttctgag caacatggaa
     5221 aaatggagtc aggaactggg catatttaca gaacaggtgg aggatgaaat agcagcacta
     5281 aatatggcat tgggtgccag ctatgcaggt gtgagagcaa tgactggatc ttcaggcgga
     5341 ggaatagcct tgatgtctga ggcgttgagt ctggcaggaa ttattgagac gccggttgtg
     5401 attataaatg ttcaaagacc agggcctgca acagggcttc ccacaaggac agaacaggct
     5461 gatttaagat ttgcaataca ttccgggcac ggtgagtttc ccaagatggt catcagctta
     5521 agaagtccag aggatgcctt ctaccagact gcgagggctt tcaacattgc agagaaatac
     5581 cagatacctg taatactcct aagtgatcag tatcttgctg atggtgaatt aaccatcgaa
     5641 ccatttgatt tcagcaagat tgaaattgaa cggaatctgt atgaagatga attgcctgct
     5701 ggcagataca agcgctacga gcttactgat accggtatat caccaagaat aattcctgga
     5761 aaagtagaag acaagttaat cctggtggat agcgatgaac atgacgaata tggaaatatc
     5821 actgaaagtg cccaaatgag gacatcaatg gtggacaaac ggagcaaaaa gggtgaactc
     5881 cttgaaggtg aggttgaaga gccttggctt atcggagatg aaaatcccga caatcttctg
     5941 gtatgctggg gatcactatt tggacaagtt aatgaagttg tagagatttt acaggaagag
     6001 ggccattcag taggtgcact ggtatatggg gatatttatc ccttcccaac aagacttctt
     6061 aaggaaaatg ctgcaacagc gaaaagtatc atcgatattg agaataattc ggcagcacag
     6121 cttgatgggc tgatgagaga atttgcactg ataaaaagtg atcacaagat acttaaatat
     6181 gatggaagag cattcagcgt tgatgaactg tacaatagac ttaaggaggt tgttgaataa
     6241 tgacatgcga atataatctt tacgacccat catggtgtcc cggttgtggc aactacatga
     6301 ttagaactgc attgaaacag gcattggagg agcttgaaat acc
//
LOCUS       DASRQF010000005         3723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_985731, whole genome shotgun sequence.
ACCESSION   DASRQF010000005 DASRQF010000000
VERSION     DASRQF010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985731"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VFD79_00130"
     CDS             complement(<1..438)
                     /locus_tag="VFD79_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HZH92481.1"
                     /translation="MHKKLFIPGPVDVLPEVLEKMATPQIGHRTKEASDLQRGISEKL
                     QKVFYTENQILLSTSSGSGLMEGAIRSFTGKRAAVFSVGAFGDRWYKMATTNNVPADL
                     FKSELGEPTTVQMVDEALATGKYDVITVTHNETSTGVMNPTAEI"
     gene            598..1143
                     /gene="cobU"
                     /locus_tag="VFD79_00135"
     CDS             598..1143
                     /gene="cobU"
                     /locus_tag="VFD79_00135"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HZH92482.1"
                     /translation="MIKMVVGGARSGKSSFAERQFMEREDVVYMATSRVEDQEMQDRV
                     DHHKRTRPEGWRTFEGSYDLFQAVGQEENYLLDCLTVLTSNIMFDMTYESERTVPIDS
                     GLQKKVEDRIFEEVASLIKAVEEKGFNLIIVTNEVGYSLVPENHIGRVFRDIQGRLNQ
                     RVAERADEVYLVTCGIPVRIK"
     gene            1143..1877
                     /gene="cobS"
                     /locus_tag="VFD79_00140"
     CDS             1143..1877
                     /gene="cobS"
                     /locus_tag="VFD79_00140"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HZH92483.1"
                     /translation="MDGLILALQFFTRIPVNKEVSFQGKNIGKSLFYLPIIGLVIGMI
                     AALPAILLSGYSKPISALLTVLIITGLTGGLHLDGLADTFDGFLSGREKNRVMEIMKD
                     STLGAFGAVALIFAIISKLVAVHELPAEYWIGIPLSLANARLVAGYVITTKKNAREDG
                     LGALFKLSRAGRSVLLSGLVLTGITLFIDPIFLIPLAGSFLLGELVSIWAYRKIDGMT
                     GDVYGAVIELCELTSLIIFWGVTIWI"
     gene            1868..2434
                     /locus_tag="VFD79_00145"
     CDS             1868..2434
                     /locus_tag="VFD79_00145"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZH92484.1"
                     /translation="MDIIFVRHGESHDNVEKRFSRDFITLTERGVQQIREARDNLAAY
                     DFTRVYVSPLKRTLQTLSHLGLDGVVDDRVREMNFGVFTGLTFNEYQDKYPQETRLWM
                     EDSFNYTIPEGESIADTYKRVESFMEDMLQKNENALVVTHEGIIRLACSWVIGGADHF
                     YRFRADNGSATVISADRNYRFIKQLNHK"
     gene            2447..3214
                     /locus_tag="VFD79_00150"
     CDS             2447..3214
                     /locus_tag="VFD79_00150"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZH92485.1"
                     /translation="MRRIVNCISTIRELDVEDLNAAGCGVELQDFVEPNLSENDMKKL
                     INEYKRSLREIKGIKSMHGPFLDLKPASPDKEIRRVSQIKYRRTLEIAHELDVDYVVF
                     HSQINPYLNQPFLRDLNNRQAAAFWRALMDVTPYKGEVVIENVFEESPYMLRDLIDAI
                     GHERISINLDIGHSNLGYTSLKDWIHVLADRLSYMHVHGNSGFEDQHIVPSDEKLDSL
                     LEILDQEGIDPFLALEYKINNLKEEVQRYRRPHSYIK"
     gene            3333..>3723
                     /locus_tag="VFD79_00155"
     CDS             3333..>3723
                     /locus_tag="VFD79_00155"
                     /inference="COORDINATES: protein motif:HMM:NF020364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZH92486.1"
                     /translation="MSATVQNYMKSRHNLSKIVVDYIKESILSGLYKEGDHILETEVA
                     NTLGISRAPVREAIKELEKEGIVQTLPRRGTFVTKFSLEDIKEVFEIRMLLENNIFKI
                     LIYENKLSDEDFINLEKLVIQMERIADS"
BASE COUNT         1134 a          685 c          914 g          990 t
ORIGIN      
        1 tatttcggca gtagggttca taacccccgt tgaggtttca ttgtgtgtta cggtaatgac
       61 atcgtatttt cctgttgcaa gtgcttcatc caccatttgg actgttgtag gctctcctag
      121 ctcagacttg aaaaggtctg cgggaacatt gttggtggtt gccattttat accatctgtc
      181 accgaaagct ccaactgaga aaactgctgc cctctttcca gtgaaggacc ttattgcgcc
      241 ttccatcagt ccactacctg agcttgttga gagtagtatc tggttttcag tataaaagac
      301 cttctgaagt ttctctgaga tacctctctg aaggtctgaa gcctccttgg ttcggtggcc
      361 tatctgcggt gttgccatct tttccagaac ctctggcagt acatcgacag gtcccggtat
      421 aaataatttt ttgtgcatag cattacctcc ttaattggtc ttgtgatagt attaccctaa
      481 aacactatca aatatacgtt taaggtcaaa aaaatctgtc aactgtgggt tttcacctct
      541 actgacactg tgataagata gaggtgggtt taaaataaga aaaaagtggt gaaaataatg
      601 ataaagatgg tagtaggtgg agccagaagt ggcaaaagct cctttgcaga gaggcagttt
      661 atggaaaggg aagatgttgt ctatatggca acctcaaggg ttgaggatca ggagatgcag
      721 gacagggtgg accaccacaa aagaaccaga ccagaaggtt ggcgaacctt cgaaggaagc
      781 tacgaccttt tccaggcagt tggacaggaa gaaaattacc ttttggattg cttgacagta
      841 cttacctcaa acatcatgtt tgatatgaca tacgagtcag aaagaaccgt ccccattgac
      901 tcagggcttc agaagaaagt ggaggacagg atatttgaag aggtggcttc gctgataaag
      961 gctgttgaag aaaagggatt caatcttatt atagtaacca atgaggttgg ctactccctg
     1021 gtgcctgaga atcacatagg cagggtattc agggatatac agggaagatt gaatcagagg
     1081 gttgcagaaa gggcagatga ggtctatctt gtaacctgtg gaatacctgt gaggataaaa
     1141 taatggatgg attgattctt gcattacaat ttttcaccag aatacctgta aataaggagg
     1201 tctcatttca gggtaagaat atcgggaaaa gtctcttcta tcttcccatt attggccttg
     1261 tcattggaat gatagcagct cttccagcaa tactgttaag tggatacagc aagccaattt
     1321 ctgccctttt aacagtactc ataataacgg gacttacagg aggactccac cttgatggac
     1381 ttgcagacac ctttgatgga tttctctcag gaagagaaaa gaaccgggtt atggagatta
     1441 tgaaggacag caccctggga gcctttggag cagttgcact gatttttgca ataatctcaa
     1501 agcttgtggc agtacatgaa ctgccggcag aatactggat tggcataccc ttgtcccttg
     1561 ccaatgcaag acttgtggct ggatatgtga ttacaactaa gaaaaatgcc agggaggatg
     1621 gcctgggggc cttgttcaag ttatcaagag caggtcggag cgtcctttta tcaggactgg
     1681 ttttgactgg gataactctc ttcattgacc caatttttct aatacctctt gctggaagct
     1741 tcctgttggg tgagctggtt tcaatttggg cctacaggaa aatagatgga atgactggag
     1801 atgtgtacgg agctgtcatt gagctttgtg agcttacttc attgataata ttttggggag
     1861 tgactatatg gatataatat ttgttagaca tggggaatcc catgacaatg tggaaaaaag
     1921 gttcagcagg gacttcataa cccttacgga aagaggtgta cagcagataa gggaggccag
     1981 ggacaatctt gcagcctatg actttaccag ggtttatgta agtcctttga aaagaacact
     2041 ccaaactctt tcgcacctgg gtcttgatgg tgtggtggat gacagggtaa gggagatgaa
     2101 ctttggtgta tttacaggcc taaccttcaa tgagtaccag gacaagtatc cccaggagac
     2161 aagactgtgg atggaggatt ccttcaacta taccatccca gagggtgaaa gcatagctga
     2221 tacctataaa agagtggaaa gctttatgga ggatatgctc caaaagaatg agaatgcact
     2281 tgttgtaacc catgagggaa tcatcagact tgcatgcagc tgggtgatag ggggtgcgga
     2341 ccacttttac agattcaggg cggacaatgg cagcgcaaca gtgatttcag ctgatagaaa
     2401 ctacagattc atcaaacagc tgaatcacaa gtagggggat cagaccatga gaagaatagt
     2461 gaactgcata agtacaataa gagagcttga tgtggaagac ctaaatgcgg ctggctgcgg
     2521 agtggagctg caggattttg tggagccaaa tctttcagag aatgacatga aaaaactgat
     2581 taatgagtac aagagaagtc ttcgggaaat caagggaata aaatcaatgc acggaccgtt
     2641 cctggacctt aagcctgcaa gtcccgacaa ggaaatcaga agggtcagtc agattaagta
     2701 cagaaggact cttgagattg cacatgaact ggatgtggat tatgttgtat ttcacagcca
     2761 gataaatccc taccttaacc agcccttcct aagggatcta aacaataggc aggcagccgc
     2821 cttttggagg gcattgatgg atgtgactcc ctataaggga gaggtggtca ttgagaacgt
     2881 gtttgaagaa tctccctaca tgcttagaga cctgattgat gcaattggtc atgaaaggat
     2941 ttcaataaat ctggacattg gccactccaa cctgggatat acatcactta aggactggat
     3001 ccatgtactc gcggacagac tttcctacat gcatgttcac gggaacagtg gctttgagga
     3061 ccagcatatt gtaccatctg atgaaaagct tgatagtctt ctggagattt tggatcagga
     3121 gggaatagac ccttttctgg cattggaata taagatcaat aatctgaagg aagaggtcca
     3181 aaggtacaga agaccacaca gttatataaa ataatgatac atgtaaatat aatttctcag
     3241 aatcttcatt tacactttga ttgtcgacaa tcttggtgtt ataataaatt tagcaatcag
     3301 caactgtaaa tgactagaga gaggagccaa ccatgagtgc aactgtacaa aattacatga
     3361 aaagcagaca taacctaagt aaaatcgttg tcgattacat caaggagtca attttgtcag
     3421 gattgtacaa ggaaggcgac catatactgg agacagaggt ggctaacacc ctgggtataa
     3481 gcagggctcc tgttcgagaa gccatcaagg aacttgaaaa ggaaggaata gttcagacac
     3541 taccaaggag aggcaccttt gttactaaat tcagtctgga agatatcaag gaagtttttg
     3601 aaataaggat gctattggaa aacaacatat tcaagatcct gatctatgaa aataaattat
     3661 cggatgagga tttcattaac cttgagaaac tggttattca aatggagaga atagctgatt
     3721 cca
//
LOCUS       DASRQF010000006        10674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_988554, whole genome shotgun sequence.
ACCESSION   DASRQF010000006 DASRQF010000000
VERSION     DASRQF010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 10674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10674
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988554"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VFD79_00160"
     CDS             complement(<1..444)
                     /locus_tag="VFD79_00160"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Catalyzes first step of the de novo purine
                     nucleotide biosynthetic pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HZH92487.1"
                     /translation="MSGVVGVYSSTNEHVSPYVYFGLYAIQHRGQISSGIAVNNNGFV
                     DYVKELGLVNEVFNNETINRLRGNIAIGHVRYAAHFEAKDKANAQPLVVGYRRGALGI
                     CLDGSIVNFRKIREDMEDGGAIFQTDLDTEVIATLIAKYHKNDFNQ"
     gene            complement(462..941)
                     /gene="purE"
                     /locus_tag="VFD79_00165"
     CDS             complement(462..941)
                     /gene="purE"
                     /locus_tag="VFD79_00165"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HZH92488.1"
                     /translation="MKVSVIMGSISDKPIGDKVIQVLEDFGVDHELRVISAHRTPFVA
                     KEFAEKAEENGTEVIVAIAGKAAHLAGVLAGLSPLPVVGIPAKSSTMDGLDSLLSVVQ
                     MPKGVPVATVAIDGGENGGLLAVQILSVKYPELRDKFKEFKEKMAQDTIAADQQLNG"
     regulatory      complement(1106..1205)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(1188..1874)
                     /locus_tag="VFD79_00170"
     CDS             complement(1188..1874)
                     /locus_tag="VFD79_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HZH92489.1"
                     /translation="MELVYKGKTKDVYKLEDGNYLLKFKDDVTGENGVFDPGANTVGL
                     SIEGAGKAGLRLTTFFFKKINEKGIPTHFVESDIDNDTMTVLPASLFGKGVEVICRYR
                     AVGSFLRRYGMYAEEGQELDSYVEVTLKDDDRNDPLITDEALEMLGIMTKEEYRVLVG
                     LTKEIGAVVKDELAKKGLELYDIKFEFGRVGEDKHIALIDEISGGNMRAFMGDEYIEP
                     LKLEQIMLND"
     gene            complement(1887..3173)
                     /locus_tag="VFD79_00175"
     CDS             complement(1887..3173)
                     /locus_tag="VFD79_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HZH92490.1"
                     /translation="MIKENNTNVKTEILAGITTFMTMAYILAVNPGMLSATGMDAGGV
                     FTATTISAIVATLIMALYAKYPFALASGMGLNAFFAFTIVLGPMGKSWQFALTAVLIE
                     GIIFILLSVLKAREAIFDSIPLNLKHAVSVGIGLFIAFIGLSGAGIVVTGDGTIVALG
                     DLASGGAAVAIIGIIITGYLLSRKVNGAILIGIIATTLIGIPLGVTIIPEGFSIVSLP
                     PSVADVAFKFVGFEEIFSLEMLVVVFTFLFVDIFDTVGTLAGVSSKAGMLDENGRLPR
                     VGKALMADAVGTIVGACLGTSTVTTYVESAAGVAEGGRTGLTSLATAGMFAIALFFAP
                     LFTMIPAAATAPALVIVGLFMMTPITKINFIDFTEAIPAFLTIVMMPFAYSIADGIVF
                     GMVSYVVLKLLTGRTKEVSKVMWVLALLFVLKFIFI"
     regulatory      complement(3339..3440)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(3694..4698)
                     /locus_tag="VFD79_00180"
     CDS             complement(3694..4698)
                     /locus_tag="VFD79_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92491.1"
                     /translation="MSIAELVNRHGEEHSPYQGNLVNHLPMGQLALFKLTGDLMATEE
                     FTRKYIQRASINPVREEFPKSDDIWKCVENRDGYEACLSLVKEELTLDNWREYTFKIM
                     DKLKFGMSSGLFHTLIRTAYAVEGMQEDEDYIHELRRAIAYYITAYKGAGAFEVSIEG
                     ERIIHEMENLLNNPNIGRLLQEEETMGMKLKALYSNPMYNKLGFLVEGESGDKVKALL
                     DLLVPAYRDTDSIVVLHCITGLHALVVLKDFFEDYSYALDIMTTCIISHLLTVEELSV
                     REEEVDLIGESWQEINNLASKSLDVHTVKLAYTAGQMDRMFDKPQLRTIAMRRIKKTK
                     "
     gene            4994..5194
                     /locus_tag="VFD79_00185"
     CDS             4994..5194
                     /locus_tag="VFD79_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZH92492.1"
                     /translation="MMQGTVKWFNAEKGFGFITGQDGKDVFAHFSQIQKDGFKTLEEG
                     QEVEFDVVEGQKGLQAENIVAL"
     gene            complement(5248..6174)
                     /gene="metA"
                     /locus_tag="VFD79_00190"
     CDS             complement(5248..6174)
                     /gene="metA"
                     /locus_tag="VFD79_00190"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: protein motif:HMM:TIGR01001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HZH92493.1"
                     /translation="MPVVIPGTLPAFEILKKEKIFVMEDARAYGQDIRPLEVCIVNLM
                     PTKIETETQLIRMLANTPLQINIHLVHMKTHESRNTRQDHLDTFYLTFEDIRNRKFDG
                     MIITGAPVENLPYEEVDYWEELVQLMEYSKENVFSTMHICWGAQAALYHHFGLKRHRL
                     DKKLFGVFKHDVTPLGGRLLRGFDDSFQAPHSRFTQIEREEFEKVPGMRILAYSEEAG
                     VHISSIADRRQIFVQGHGEYDRETLKKEYERDLLKGSAQLPVNYFHNDDPQGEIEINW
                     KSHSQLLFSNWLNYCVYQETPYNIEEMEPIKY"
     gene            complement(6191..7474)
                     /locus_tag="VFD79_00195"
     CDS             complement(6191..7474)
                     /locus_tag="VFD79_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HZH92494.1"
                     /translation="MKNYSTETKCVQGTYKPKSGQPRVLPIVQSTTYQYYDSDDVADL
                     FDLKSADHMYSRISNPTVAEFEGKMALLEGGVGAVAASSGQAATTLAILNICNAGDHI
                     IAASTIYGGTFNLLGVTLKKLGIQVTFVDPDSSEEEIIASGRENTKAVFGETIGNPGL
                     NILDFEKFSRVAKTLDVPFIVDNTLATPVLCRPFEHGVDIVIHSATKYTDGHATSVGG
                     IVIDGGTFNWDNGKFPELVDPDPSYHGISYVKDFGPAAYITKARVQLLRDFGMTLSPF
                     SAFLFHLGLETLHLRMERHCENALALAEFLQGHPGVEWVNYPYLETHKTYGTAKKYLK
                     GGSGILTFGIKGGAQAGKELVKHLDLTGLVVHLGDLRTSILHPASTTHRQLTEDQQIA
                     AGVSPDLIRVSVGIEGIDDIIADFDNAINQTTKGI"
     regulatory      complement(7551..7651)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(7718..9718)
                     /locus_tag="VFD79_00200"
     CDS             complement(7718..9718)
                     /locus_tag="VFD79_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZH92495.1"
                     /translation="MKVEHGGDSQEMGAQVRKIVLFFEPHVEVLERDRDHDKDQGHEG
                     EEGHSHGHNHGSKDMRGVILGTALFVAALVLPLEGLPSLLLYLGAYLLIGGEVILRAF
                     RNLTRGQVFDENFLMTIATMGAFAINEYPEGVAVMLFYQVGEFFQDYAVNKSRRSIAE
                     LMDIRPDYANFVDGDEVRKVDPKRIKKEDLILVRPGEKVPLDGLVVEGSSSMDTAALT
                     GESMPRDVNMGDPVLSGFINRQGLLKVKVEKEYNESTVSKILDLVENAASKKAPTENF
                     ITKFARYYTPAVVFFALGLAIVPPLLIQGALFSDWIYRALIFLVVSCPCALVISIPLG
                     FFGGIGGASRHGILVKGGNYLEALNSVSTVVFDKTGTLTEGEFQVVEVVSTGGLAEED
                     LLYLAALGESHSSHPIAQSVLKANKRDLRVGLVDDYKELAGLGVLAHVDGQELLLGNS
                     SLFTEKGIATEGEDNGKTIIHIALDNVYQGYILIADKVKEGASLAIRELKERGVENLV
                     MLTGDRDQVAKEVARDLGIDKVFSELLPQDKVGVIEDLTDGLRKNRKLIFVGDGINDA
                     PVLARADIGVAMGALGSDAAIEAADIVLMTDDLAKLPVALDIASWTRRIVWQNIFFAL
                     GVKAIVLVMGALGVATMWEAVFADVGVALIAVLNAMRVLRHA"
     gene            complement(9856..10221)
                     /locus_tag="VFD79_00205"
     CDS             complement(9856..10221)
                     /locus_tag="VFD79_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018306620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZH92496.1"
                     /translation="MSTDKNIAVCDCTVIHEETVNKVRGNMPPDQLLDLSADFFKVVG
                     DRTRMRILHALFQSEMCVCDIAVLLEMNQSAISHQLRVLKQAGLVKYRREGKIVFYSL
                     DDEHVMNILDQGLVHVGHK"
     gene            complement(10353..>10674)
                     /locus_tag="VFD79_00210"
     CDS             complement(10353..>10674)
                     /locus_tag="VFD79_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92497.1"
                     /translation="GLPEGGKRLAAMLDVDQNMYMLITETELVEGEVEITLTQEGLSF
                     MSVKDAVEIFGLQMEPSEELMHIRELDNHGYDIEWNEQLRMIEVYFEMPEPALEELLE
                     ESVE"
BASE COUNT         2671 a         2758 c         2117 g         3128 t
ORIGIN      
        1 ctggttgaag tcattcttat ggtatttggc aataagggtg gcgataacct ctgtatccag
       61 gtcggtctgg aagatggctc ctccatcctc catgtcctcc ctgatcttcc tgaagtttac
      121 tatgcttcca tccaggcaga ttcccagggc tccccttctg tatccaacga ccaggggctg
      181 tgcatttgcc ttgtccttgg cctcaaagtg agctgcatac cttacatggc caattgcaat
      241 gtttccccta agcctgttga ttgtctcatt gttgaagacc tcatttacaa gaccaagttc
      301 cttgacatag tccacaaacc cgttgttgtt tacagctatt ccagagctga tctgacctct
      361 gtgctgaatt gcataaagac cgaagtagac ataaggagat acgtgttcat ttgtactgct
      421 atatacccct actacaccac tcatctatta ttaacctccc cttatccatt gagctgctgg
      481 tcagcagcta tggtatcctg agccatcttt tccttgaatt ccttgaattt atccctcaat
      541 tccggatact tgactgaaag gatctgtaca gccaagagtc caccattttc tcccccgtct
      601 attgcaactg tggctacagg aacccccttt ggcatctgaa ccactgaaag aagtgaatcc
      661 aacccatcca ttgtagatga cttggcaggg atacccacaa caggaagggg tgaaagtcct
      721 gccaggacac cagccaggtg tgcggccttg cctgcaatgg cgactatgac ctctgttccg
      781 ttctcttcag ccttttcagc aaactccttt gcaacaaagg gtgtcctgtg ggctgaaatt
      841 accctcagtt catggtcaac gccaaaatcc tcaagaacct ggatcacctt gtccccaatt
      901 ggcttatctg aaatgcttcc cattattacg cttaccttca tatcctgaat cctcctttaa
      961 tatattcaat gtgattatac ccaagtaaca ttaaagccca gacgggtagg cttaagaaac
     1021 ctacccgtct gggcttttct ccctccggtg tgatggcctt tactgccacc tgtaccgctt
     1081 ggaccagctg agaagatctc acggaaccct aggtacactt tctcatagac aatgatttac
     1141 ggtcattggt agaaacgtcc tccccctatc agaggacata tatgagttta gtcgtttagc
     1201 attatctgtt caagcttaag tggctcgatg tactcatctc ccataaaggc cctcatattt
     1261 ccgcctgata tctcatctat cagtgcaata tgcttgtctt cccccactct accgaactcg
     1321 aattttatgt catagagctc aagacccttc ttggcaagct catcctttac aactgctcca
     1381 atttcctttg ttagtccaac caggaccctg tattcttcct tggtcatgat acccagcatt
     1441 tccagtgcct cgtcagtgat aagcgggtcg tttctgtcat catccttaag tgtgacctcc
     1501 acataggaat caagctcctg cccttcctct gcatacatac cgtatcttct caggaagctt
     1561 ccaacagccc tgtatctgca gataacctca acccccttgc cgaaaaggct ggcgggaagc
     1621 actgtcatgg tgtcgttgtc aatgtctgat tccacaaagt gtgtgggaat ccccttttca
     1681 ttgatcttct tgaagaagaa ggtcgttagt cttagtcctg ccttgccagc tccctctatg
     1741 ctcagtccaa ctgtgtttgc tccaggatca aacactccgt tttctcctgt tacatcatcc
     1801 ttgaacttca aaaggtaatt tccatcctca agcttgtata catccttggt ctttccctta
     1861 taaactaatt ccataatcca tacctcctag ataaatatga atttaagcac gaacagtaag
     1921 gcgagaaccc acattacctt tgaaacttcc ttggtacgtc cagttaaaag cttaagcaca
     1981 acatatgaaa ccattccgaa tactattccg tctgcaatgc tgtatgcaaa tggcatcatg
     2041 acaattgtca agaatgctgg tattgcctct gtgaaatcta tgaagttgat ctttgttatt
     2101 ggtgtcatca tgaacagtcc cactattaca agtgcaggag ctgttgcagc tgccgggatc
     2161 attgtaaaca agggtgcaaa gaaaagtgcg atggcaaaca ttcctgctgt ggcaagggat
     2221 gtaagtcctg ttcttcctcc ctctgcaact ccggcggcac tttcaacata tgttgttact
     2281 gtgcttgttc caaggcatgc accgacgatt gtccctactg catctgccat aagtgccttt
     2341 ccaacccttg gaagcctgcc gttttcatca agcattccag ccttggagga tacccctgcc
     2401 agtgttccaa ctgtgtcgaa tatatccaca aagaggaatg tgaatacaac cacaagcatc
     2461 tcaagtgaga atatctcctc aaatccaacg aacttgaagg ccacatccgc cactgacggt
     2521 ggaaggctta caatgctgaa tccctctgga ataattgtaa ctcccaatgg gatccctatt
     2581 aatgtggttg ctatgatacc gatcagtatg gctccgttta ctttcctgga taggaggtag
     2641 cctgtgataa tgattcctat gattgcaact gctgcaccac ctgaagccag gtctccaagg
     2701 gcaactattg ttccatcgcc tgtaactacg attccggctc cgcttaggcc gataaaggct
     2761 atgaaaaggc cgattccaac cgataccgca tgctttaggt ttagaggtat tgagtcaaat
     2821 attgcctctc ttgcctttag aactgataat agtataaaga ttattccttc aatcaatact
     2881 gctgtcaatg caaactgcca tgatttcccc atgggtccaa gtacgattgt aaatgcgaag
     2941 aatgcattaa gacccattcc tgaggccaat gcaaatggat actttgcata aagtgccatg
     3001 ataagtgttg caactatagc cgatatggtc gttgcggtaa atactccacc agcgtccatt
     3061 cccgtagctg aaagcattcc agggtttaca gccagtatgt aggccatagt catgaaggta
     3121 gtgataccag ctaggatttc tgtcttgaca ttagtattgt tctctttgat cataaaaaac
     3181 ttttccaacg aaagttcctc cttaaatttt tttcgcggaa aaaaatatag cccaggtggg
     3241 taggtctagc gaaacctacc cgcctgggct tttatccctt cggtgtgaca cgaaatgcat
     3301 ctgtaccgct tggaccagct gagaatttct cacggaaccc taggtacact ttcgctcata
     3361 gtgcagtgat tatcggtcac caactagaaa catcccaacc gtattatggg acctatatga
     3421 gccatattta attcacactt tgagtttaac agtttgctca ccctatgtca acctgataat
     3481 ttaaaacata ttccccatta taatgcctcc atttagccat ttataactgt aatccaaggc
     3541 agcgtccttg tatttccaag gtattgagta gtccttccat atgaagctgt ggcacctcca
     3601 ctgatgaaat atcatttcaa actctcgtgg tgaaactctt cagtttcgct gccgcaaatc
     3661 ttgcatccta ttgtcatctt tcctccctgc agcctatttt gtctttttta tccttctcat
     3721 ggctattgtc ctcagctgag gcttgtcaaa catcctgtcc atctggcctg cagtataggc
     3781 aagctttaca gtgtgtacat caagggattt tgaggcaaga ttatttattt cctgccagga
     3841 ttctccaatg aggtccacct cttcctccct tacgctcagt tcctccaccg taagaagatg
     3901 tgatattatg caggttgtca ttatatccag ggcataggag tagtcttcaa agaagtcctt
     3961 aaggaccacc aaggcgtgaa gtcctgttat gcagtggagt acaacgatgc tgtcagtatc
     4021 cctgtaggcc ggaaccagaa ggtccaaaag agcctttacc ttgtccccgg attcaccctc
     4081 aaccaagaac ccaagcttgt tgtacattgg attggagtag agggccttaa gcttcattcc
     4141 catggtctcc tcttcctgta aaagcctccc tatgtttggg ttgttcaaga gattctccat
     4201 ctcatggatg atcctttccc cctcaatgga cacctcaaag gctcccgccc ccttgtaggc
     4261 agtaatgtaa taggctatgg cccttctcag ctcatggatg tagtcttcat cctcctgcat
     4321 tccctccaca gcatatgcag ttcttataag tgtatggaaa agccctgatg acattccaaa
     4381 cttgagcttg tccataatct tgaaggtata ttccctccag ttatcaaggg tcagctcctc
     4441 cttcaccaga gacaggcatg cctcgtagcc atccctgttc tctacgcatt tccatatgtc
     4501 atcggatttg ggaaattctt ccctcactgg attgatggag gctctctgaa tatatttcct
     4561 tgtgaattct tcagtagcca taagatctcc agtcagcttg aaaagtgcca attgacccat
     4621 aggcaggtgg tttacaagat ttccctgata tggagaatgc tcctccccgt gacgatttac
     4681 taattcagcg atactcatac ggctcctcct tacacacatt gctaaaagca aaattttctc
     4741 tccacttcta ttttaccaaa ttaaagtctt taagtaaaat ctccctggtt tttcattata
     4801 gagggacctt ttaattaatt tgcaaataaa gcttgcttta tccagctgca tgtgttaacc
     4861 ttaagtagaa cttgaattaa gagtaatagc aacacaatag atatttcctg cggcagtgcc
     4921 ttgaggtaca cctgtaacga aattttatta gttagttatt tacgatgatt caacaaaaaa
     4981 atttcggagg tgcattatgc aaggtacagt aaaatggttt aacgcagaaa aaggatttgg
     5041 atttatcaca ggacaagacg gaaaagatgt tttcgcacac ttttcacaaa ttcaaaaaga
     5101 tggattcaaa acacttgaag aaggacaaga agtagagttt gacgtagtag aaggtcaaaa
     5161 aggacttcaa gcagagaata tcgtagctct ttaattaaac gataatcaat ccctgagcca
     5221 atggctcagg gatttctttt ttttgcctta atatttgatt ggttccattt cctctatatt
     5281 gtaaggcgtc tcctggtata cgcaatagtt tagccaattg gaaaagagaa gctgtgagtg
     5341 actcttccag tttatctcaa tttcaccctg tgggtcgtca ttgtgaaaat agttcactgg
     5401 cagttgggca cttcccttta gcaggtctct ttcgtactcc ttctttaagg tttccctgtc
     5461 atactccccg tggccctgaa caaatatctg cctcctgtcc gctattgatg aaatatgaac
     5521 tccagcctcc tctgagtagg ccagtattct cattcctggc accttttcaa actcctccct
     5581 ctcaatctgt gtaaaccttg aatggggggc ttggaagcta tcatcaaagc cccttaggag
     5641 ccttcctcca agaggggtca catcgtgctt gaagaccccg aagagcttct tgtccaaacg
     5701 atgcctttta aggccaaagt ggtggtagag ggcagcctgg gcaccccagc agatatgcat
     5761 tgtgctgaat acattctcct tggagtactc cataagctga acaagttcct cccaatagtc
     5821 cacctcctca tagggaaggt tctcaacagg tgcacctgtt attatcatgc catcgaactt
     5881 cctgttcctt atgtcttcaa aggtcagata gaaggtatcc aggtggtctt gcctggtgtt
     5941 ccttgattca tgggtcttca tgtgcacaag gtggatgttt atctgaagtg gtgtgttggc
     6001 cagcatcctt atgagctgtg tctcagtctc gatttttgtg ggcataaggt ttacgatgca
     6061 tacttcaagt ggccttatgt cctggccgta ggccctggcg tcctccatta caaatatctt
     6121 ttccttcttg agtatttcaa aagccgggag agtccccgga atcacaactg gcatttgtca
     6181 tcctccttgc ctaaattccc ttggttgttt gatttattgc attgtcgaaa tccgctatta
     6241 tatcatcaat gccttcaatt cccactgata cccttataag gtcagggctt acccctgcag
     6301 caatttgctg gtcttctgta agctgcctgt gggttgtgct ggcaggatgg agtatgctgg
     6361 ttctaaggtc tcccagatga accacaaggc ctgtaaggtc aaggtgctta accagttcct
     6421 tgcctgcctg ggcacctccc tttatcccaa aggttaggat cccgctgcca cccttcaggt
     6481 atttcttggc ggtcccgtag gtcttgtgcg tttcaaggta gggatagttt acccactcaa
     6541 caccgggatg tccctgaagg aactctgcaa gtgccagtgc attttcacag tgtctttcca
     6601 ttctaagatg aagggtttcc agtcccaggt gaaagaggaa ggcactgaag gggcttaggg
     6661 tcattccaaa gtcccttaag agctggactc gcgccttcgt tatatatgct gcaggcccaa
     6721 aatccttgac atagcttatg ccgtggtaac ttggatcagg gtccaccagt tcagggaact
     6781 tcccgttgtc ccaattgaag gtccctccat ctatcactat tcctccaacg cttgtggcat
     6841 ggccgtcggt gtactttgtt gccgagtgga tgactatgtc cacaccgtgt tcaaatggcc
     6901 tgcaaagaac tggcgtcgcc agtgtattgt caacaataaa gggcacgtcc aaggtctttg
     6961 caaccctgct gaacttttca aaatcaagga tgttaagacc cgggtttcca attgtctccc
     7021 caaaaaccgc cttggtgttt tcccttcctg aagcaattat ttcctcctcg gatgaatccg
     7081 ggtccacaaa tgtcacctga attccaagct tcttaagtgt aaccccaaga aggttgaatg
     7141 ttcctccgta gattgtcgat gcggctatta tatggtctcc cgcattgcag atattaagga
     7201 tggccaatgt tgttgcagcc tgtcccgatg aagcagccac tgcccctacc ccaccttcaa
     7261 gaagtgccat cttgccttca aactctgcaa cggttgggtt gctgattctg gagtacatgt
     7321 gatcagcact cttaaggtca aataggtctg ccacatcatc agagtcatag tactgatagg
     7381 tcgtggactg gacaattgga agcacccttg gttgtcccga ttttggcttg taggttccct
     7441 gtacgcattt tgtttctgtt gaatagtttt tcatgtcttc ctccttctaa ctgtaacttg
     7501 tcaaacaaaa aaagaaaacc gcttcaataa gaagcggtag gtaccttttc ttcttatctc
     7561 ccagaattaa ctgttggaat tggcaccttg cgcatttgcg taggttgccg aagcttcaca
     7621 gggccattcc ctccacttct cttgataagt cacaattatt aatttttagt atttcatatg
     7681 attctatatc ctttaccccg gcttgtcaag agttttttta agcgtgtcgc aaaaccctca
     7741 ttgcattcag gacagctatt agggcgactc caacatcggc aaaaactgcc tcccacatgg
     7801 ttgcaactcc caaagcaccc attacaagaa ctatggcctt tactccaagt gcaaagaaga
     7861 tgttctgcca aactatcctt ctggtccagg aagcgatgtc aagggccact ggaagctttg
     7921 caaggtcatc tgtcataagg actatgtcag ctgcctcgat ggcagcatca gacccgaggg
     7981 cccccattgc aacccctata tcagcccttg caagaactgg agcgtcgttt attccatctc
     8041 ccacaaatat aagttttctg ttcttcctaa ggccatctgt caggtcttca attacaccta
     8101 ccttatcctg tggcaggagc tccgaaaaca ccttgtctat tccaaggtcc ctggcaacct
     8161 ctttagccac ctggtcccta tcccctgtga gcattacaag attctccacc ccacgttcct
     8221 tcaactccct gatggctaga cttgctcctt ccttgacctt gtcggctatt aggatgtagc
     8281 cttggtagac attgtccaat gcaatatgaa taattgtttt gccattatcc tcaccttccg
     8341 ttgcaattcc cttttccgta aaaagcgaag aatttccaag aagcagttcc tgaccgtcaa
     8401 catgtgcaag gacccccagt cccgcaagct ccttgtagtc atcaaccagc cccaccctta
     8461 ggtctctttt gttggccttt agtacggatt gggcaattgg atgggaggaa tggctttctc
     8521 caagggctgc aaggtaaaga aggtcttcct ctgccaatcc tcctgttgaa acaacctcca
     8581 ccacctggaa ttccccttca gttagggttc ccgtcttgtc aaataccact gtggacactg
     8641 agttcaaggc ctcaagatag tttcctccct ttacaaggat cccatgtctg gacgcccccc
     8701 caatccctcc gaaaaatccc agaggaattg atattacaag tgcacagggg caggacacca
     8761 caaggaatat cagggccctg taaatccagt ctgaaaagag ggctccctgg atcagcaatg
     8821 gtggaactat tgcaagccca agggcaaaga ataccactgc aggtgtgtag tacctggcaa
     8881 acttggttat gaagttttct gttggagcct ttttggaggc tgcattttca accaggtcca
     8941 gtatctttga cacagttgat tcattgtatt ccttttccac cttaaccttc agtagaccct
     9001 gtctgttgat aaatccactc agaacagggt cacccatgtt tacatccctt ggcatggact
     9061 cccctgtaag tgctgcagta tccatggatg aagacccctc caccacaaga ccgtcaaggg
     9121 gaaccttctc cccgggacgt accaggataa ggtcctcctt tttgatcctt ttgggatcca
     9181 ccttccttac ttcatcgcca tccacaaaat ttgcatagtc aggtctgata tccataagtt
     9241 cagctatcga ccttcttgat ttgttgactg cataatcctg gaagaactca cctacctggt
     9301 aaaacagcat aactgcaact ccctcaggat attcatttat ggcaaaggcc cccatggttg
     9361 caattgtcat aaggaagttt tcatcaaaaa cctggcctct ggtaagattc ctgaaggctc
     9421 ttaggatgac ctcgcctccg attagaaggt aagctccaag atacaagaga aggcttggga
     9481 gaccttcaag tggcaatacc agggcagcaa caaacaaagc cgttccaagg atcacacctc
     9541 tcatgtcctt tgatccgtga ttatggccat ggctatgccc ttcctcccct tcatggccct
     9601 ggtccttatc atggtcccta tccctttcca aaacctccac atgaggttca aagaaaagga
     9661 cgatttttct tacctgggcc cccatctcct gggaatctcc tccgtgctca accttcaaag
     9721 tcttgtttat gaaattcagg gaggcatcag aaacctccgg cagctgcttt attctggctt
     9781 ctatcctaga agcacagtta gctcagtcca tgccctccag ataaaattcg attctgttca
     9841 ttttcatgcc tccatctatt tgtgtcctac atggaccaga ccctggtcca atatgttcat
     9901 tacatgctca tcatccaggg agtagaaaac tatctttccc tctcttctat acttgaccag
     9961 gccagcctgc ttaagcaccc tcagctgatg ggatatggca gactgattca tctcaagaag
    10021 taccgctatg tcgcatacac acatttccga ctggaacaag gcgtgaagaa tccgcatcct
    10081 ggtcctgtct ccaacaacct tgaaaaaatc agcagaaagg tccagaagct ggtctggcgg
    10141 catatttccc ctgaccttgt tcactgtttc ctcatggatg accgtacagt cgcatacggc
    10201 tatattttta tctgtactca taattcttac ctcttttcat atgaatatct gttcatgtgt
    10261 tcaagtatat tttactagct ttgacagcat tttgcaagcc catataaaaa taaccgcctc
    10321 catcggagac ggttatcttc aagtatatta aattattcta ctgattcttc cagcagctct
    10381 tcaagtgctg gctctggcat ttcaaaataa acctcgatca ttctaagctg ctcgttccac
    10441 tctatgtcat agccgtggtt gtcaagctcc cttatgtgca tcaattcctc tgaaggctcc
    10501 atttggagac cgaatatctc aactgcatcc ttaacgctca taaatgaaag gccttcctgg
    10561 gtcagggtaa tttcaacctc tccctccaca agctcagttt cagtaattag catatacata
    10621 ttttggtcaa catccagcat ggctgcaagc ctctttccgc cttcaggaag tccc
//
LOCUS       DASRQF010000007         2158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1240976, whole genome shotgun sequence.
ACCESSION   DASRQF010000007 DASRQF010000000
VERSION     DASRQF010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240976"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1196
                     /locus_tag="VFD79_00215"
     CDS             171..1196
                     /locus_tag="VFD79_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HZH92498.1"
                     /translation="MNIAEYKDIYVFCEERENKIHDSSLELLGEAVRLVKDRPSLNYK
                     VVGVVIGDHSDELAELAGYYGADRVIFLHNENLKHYSTEIYTELFMDVIKNDKPDIIL
                     IPATVLGRDLAPRIAARCDTGLTADATKLDFDPENEDSALLYVTRPAFGGNLFGTIIN
                     ETKRPQMTTIRPGVMEPIHKDTEREFHLDKREVSIDHIEDKVKLIEVLQKDNMAVDIT
                     KADIILSGGRGIGDNFHVLEECAKMIGAEVGASRGAVDKGFIGKDHQVGQTGKTVRPK
                     VYIACGISGAVQHLAGMEKSDYIIAINKDPEAAIFSVANIGLVGDALEILPLLTEEIK
                     TYRERPY"
     gene            1385..2128
                     /locus_tag="VFD79_00220"
     CDS             1385..2128
                     /locus_tag="VFD79_00220"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HZH92499.1"
                     /translation="MFRKSSKLMIVLSLVLILSACSGSSDTGNVEFETGNPIAMVNGV
                     EIFQGPYERAVQRMIAGYQQQGLSLEGEMGDTVKERIHEQVLNQMIQVEVMTQEAQKL
                     GLAPNDAVVQGEIDSFRDHYGSDEEYQAALSDNLYSEEDFRKLILEDLSVMALFDEFV
                     GEVTVTEKEVEDYYLELVQEAQDQIDARKAAGEEITQEQMAMMAPPLFEEIRDDLMNQ
                     LVQLRTQEKQMEYLNGLMENSDIEILIEE"
BASE COUNT          665 a          396 c          542 g          555 t
ORIGIN      
        1 tcctgcaatc attggactca agggttcacc taccaaggtc aagaagacct tcaccaagga
       61 ggtccactca aattcagata tattgaatct tgatggcaag tcctcggccc gtaaggttgc
      121 agagatactt atggatatga attttatagg aaaataagga gggattgaat atgaacatag
      181 ctgaatacaa agatatatat gtcttctgcg aggaacgtga aaacaagatc cacgattcat
      241 ccctcgagct tctgggagag gcagtaagac ttgttaagga cagaccttct cttaactaca
      301 aggttgttgg agtggtcatt ggagaccact cagacgagct tgcagaatta gctggatatt
      361 atggtgcaga cagggtaatc tttttacaca atgaaaacct caagcactac agcacagaga
      421 tctacacaga gttattcatg gatgtcataa agaatgacaa acctgatatc atactgatac
      481 ctgcaacagt ccttggaagg gaccttgctc cgaggatagc tgcaaggtgt gacacagggc
      541 ttacagctga tgccacaaag cttgattttg accctgaaaa cgaggactcg gcacttttgt
      601 atgtaacaag accggccttt ggaggaaacc tctttggtac aatcataaac gagaccaaga
      661 gacctcagat gacaacaatt aggccagggg ttatggaacc tatccacaag gatactgaaa
      721 gagaattcca ccttgacaag agggaagtca gtatcgacca tattgaggat aaggttaagc
      781 tgattgaagt gctgcaaaag gacaatatgg cagtggatat tacaaaggca gacataatcc
      841 tgtctggtgg aaggggaatc ggtgacaact tccatgttct ggaggaatgt gccaagatga
      901 ttggagctga ggttggagct tcaaggggag ccgttgacaa gggctttatc ggaaaggacc
      961 atcaggttgg tcagacggga aaaaccgtta gaccaaaggt ctatattgcc tgcggaattt
     1021 caggagctgt tcagcacctg gcagggatgg agaagtctga ctatatcatt gccatcaaca
     1081 aggatccgga ggctgcaata ttcagtgttg caaatattgg tcttgttgga gatgcacttg
     1141 agattcttcc actgctaaca gaggagatta agacatacag ggaaagaccc tattaaattg
     1201 aataagttta atttgaaacg aggcaattgc ctcgtttctt ttaatttgac cctatatgag
     1261 agttttcgct aacaattata atttttcaat gtgggattag tcacattcct atcaatcgtt
     1321 gtaatggaca ccttcttatg gtagtattat ccataagtgc agcatttacg aaaggggttt
     1381 gattatgttt agaaaatcaa gtaaattaat gatagtgctt tccctggtcc ttattctttc
     1441 agcctgttca ggcagcagcg acacaggaaa tgttgaattt gaaactggca atccaattgc
     1501 aatggtcaat ggggttgaga tttttcaggg tccctatgaa agggctgtcc aacgaatgat
     1561 tgcaggctat cagcaacagg gacttagcct tgaaggagag atgggagata ctgtaaagga
     1621 aaggatccat gagcaggttc ttaatcagat gattcaggtg gaagttatga ctcaagaagc
     1681 tcaaaaactt ggattggctc ctaacgatgc agttgttcaa ggagagattg attcctttag
     1741 agaccattat gggtcagatg aggagtatca ggctgctctt tcggacaatc tttactcaga
     1801 ggaggatttc agaaaactca tacttgagga ccttagtgta atggccctgt tcgatgagtt
     1861 tgtcggagaa gtaaccgtaa cagaaaaaga ggttgaggat tactacctgg agttggttca
     1921 ggaggctcaa gaccagattg atgcccgtaa ggctgctggc gaagagatta cccaggaaca
     1981 gatggcaatg atggcccctc cccttttcga ggaaattcgg gatgacctta tgaatcagct
     2041 tgtccaattg agaacccagg agaagcaaat ggaatatctg aatgggctga tggagaatag
     2101 cgatatagaa attctgattg aagaatagta aaaagtgtcc agggggactg tccccccc
//
LOCUS       DASRQF010000008        10626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_364103, whole genome shotgun sequence.
ACCESSION   DASRQF010000008 DASRQF010000000
VERSION     DASRQF010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 10626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10626
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364103"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VFD79_00225"
     CDS             complement(<1..635)
                     /locus_tag="VFD79_00225"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HZH92500.1"
                     /translation="MNESTQLLLATGAGFVLSSFLGPRIIPLLQRLKIGQSIREDGPS
                     SHLAKSGTPTMGGIIFLIAFVAATLMAGGLNAQMGVIIFSTLGFGAVGFMDDYIKVVK
                     KRNLGLRAYQKIIGQFLVAMVLIYYYLNHSGSYPQILIPLLGETNLALGVLTIPFLLV
                     VVIGTVNAVNLTDGLDGLASGVSAIVFGAFGLLSLRLGYNDVAVASFAMTGA"
     gene            complement(757..2778)
                     /locus_tag="VFD79_00230"
     CDS             complement(757..2778)
                     /locus_tag="VFD79_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZH92501.1"
                     /translation="MHEINKSTKKRLRFAILVVTLIVFFLGYRLVRVQVIEGAEYKRG
                     AMQQWTKAINLKSDRGIIYDRKGRKLAVNVTSFTVWASPSDVDNPEEAAMRLQPILKV
                     DSSEILESLRRDSPVVKLKQWITREEAEMVRELGIGGISIVDDNKRYYPFGNFASYIL
                     GFTDIDNNGLYGIERVYDQYLSGVPGIWVKATDRDNRQLPYGSERIHNPEDGTSVVLT
                     IDEIIQGFAEEAAQEALEKNQAKSVSIIVMDPATGEVLAMANKPDYDPNNPRMALDED
                     LNNQWQALPFEDQQQKWFEMWRNYAINDIYEPGSTFKIVTAAAALEEGSANLDSHYYC
                     NGFIRDIPGVVLRCSSWYDPHQDQDFSTAFSNSCNVAFVNMARELGTEKMYEYIKAFG
                     FGEVTGIDLLGEQRGIIPSGTESIREVNLATLSYGHGIAVTPIQMVNAISVIGSGGNL
                     MTPHIVKGLADSDGNIVDLFEPEIRRQVISEATANELLDMLELTVLEGTGRRAYVPGY
                     RIGGKTGTAQKIIDGRYVDGKYISSFGGLAPVDDPRLAVLVIVDEPKGIYYGGTVAGP
                     PAASVIEKTLNYLEVPRKYTDEELEATKETVLVPDVRNISINRAGKELTDLELKYTTE
                     YEDFNTDTLVLDQYPSPGVEVSKGSIIDLYLEDGRSNPVLTNEDNDTSN"
     gene            complement(2790..3236)
                     /locus_tag="VFD79_00235"
     CDS             complement(2790..3236)
                     /locus_tag="VFD79_00235"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HZH92502.1"
                     /translation="MMAAKALYDQYPEYIGEKKQKTVRKVVRRKSHALNRQMYIALAI
                     ILLSTSLFILQGYAKITSMRLEISKLENEATELAKLKQDLSGTMEGLKNTTLIGEQAK
                     NSLGMIYPEEGQIEYITVNETVTIDAYQASFQDKIRNVIGLFASLY"
     gene            complement(3260..4192)
                     /gene="rsmH"
                     /locus_tag="VFD79_00240"
     CDS             complement(3260..4192)
                     /gene="rsmH"
                     /locus_tag="VFD79_00240"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HZH92503.1"
                     /translation="MEFHHVSVLLNEVLEGLAIKEDGIYVDGTLGGAGHSREIVKRLT
                     SGRLVGIDQDLNALEKSREVLKDHIDKVTLVHSNYVNMKEVLKDLNIEKVDGILLDLG
                     VSSHQLDTEERGFSHNKDAPLDMRMDTTSDFSAWDVVNNYSSEELTRIFLEYGEEKWG
                     KRIAEFIVRERSEKPIDTTMELVTVIKKAIPKKVRMEGHHPAKKTFQAIRIEVNGELD
                     VLKRAIGEMVECLNPGGRLAIITFHSLEDRIVKDQFKDLYKDCICPPEFPTCICQKRR
                     EIEIITRKPIVPGEEELNANPRSRSAKLRVAERI"
     gene            complement(4222..4653)
                     /gene="mraZ"
                     /locus_tag="VFD79_00245"
     CDS             complement(4222..4653)
                     /gene="mraZ"
                     /locus_tag="VFD79_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004825608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HZH92504.1"
                     /translation="MFIGEYQHTIDGKGRLIIPSKFRDPLGDEFVMTKGLDNCLFVYP
                     KDEWKIVEDKLKSLPMTNKDARAFVRFFFSGATECILDKQGRVLIPGNLRDHSKLLKD
                     TVVIGVSTRLEIWSKEEWDSYNDDDSLSYDSIAEKMAELGI"
     gene            complement(4863..5600)
                     /gene="lgt"
                     /locus_tag="VFD79_00250"
     CDS             complement(4863..5600)
                     /gene="lgt"
                     /locus_tag="VFD79_00250"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005956399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HZH92505.1"
                     /translation="MDPVAFSIFGLEVRWYGLLIAIAVMIGTLIATKEAGRKGIKEET
                     FIDFLLFAVPVAILFARLYYVVFSWDYYSSNPGQILNIRGGGLAIHGAVIGGALVAII
                     FTRIKKLKFWTLADVAAPSLILGQAIGRWGNFANQEAHGGPTDLPWGIMVDGVKVHPT
                     FLYESIWNLLVFMFLIWYRKKSKVEGEIFLLYVILYSLARFFIEGLRTDSLMLGSFRV
                     AQLISVLIIVVGGALLHRKRKTSEGSL"
     gene            complement(5615..6910)
                     /locus_tag="VFD79_00255"
     CDS             complement(5615..6910)
                     /locus_tag="VFD79_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZH92506.1"
                     /translation="MKTIRNENLREEIYHKELENGINLFYFPKKGFTKKYAIFTANYG
                     STDNVFLPLGENEPVELPEGIAHFLEHKLFEEPDEDIFQKFSSQGAYVNAYTNFNQTS
                     YLFYSTDNFYQNLELLVKFVQSPYLTEENVEKEKGIIAQEIQMYQDNPKWVVFFNTLK
                     AMYHRHSVRTDIAGTVESIHKITKQDLDTAYRTFYNPGNMVLFVVGDLDGDEIIRTVE
                     NAGKEYPTTLDRVERIVADEPDEILLKSIEERMMTSKPLFNIGVKDRDLGLTGKEAVK
                     KDIITNMILDMLFTSSSEFYNELYSEGLVDSSFGAYYTGKRTYGHSLIAGQSDKPQEV
                     YNRIMAMFKKPERYLTQEAFSRIKKKEIGGFLTGFNSIEFIANNLTDLYFQDFLLIDY
                     LDVLNEITFEDIITRFESHFTEEKSVISIIWPEMESGNN"
     gene            complement(6897..8183)
                     /locus_tag="VFD79_00260"
     CDS             complement(6897..8183)
                     /locus_tag="VFD79_00260"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZH92507.1"
                     /translation="MESTRKEIGNGLNINLTYTDKFKSNYLGVYMIRPLDRAEVTLNS
                     LLPMVLERGTKNHPSTLSLKRRLEELFGANLRISASRRGERQIIKFSMEWADGRYTGE
                     EGLDSDILQMFREVIFDPYMEDGSFSREYLEQEKNNLITRINNKINDKRTYAINRCIE
                     EMCKGEKFSIYPGGYQEDVPQIDGKILYDHYRKVLSTSQIEIVYTGHDNNLNLEGIIP
                     EEFMDREAVTFVPRETIVEKSQNRNQVRDRMSVAQGKLVLGYRAGIPYEDKLYNGLLL
                     GNEIFGGGPNSILFRNVREKHSLAYYVSSSIVKHKSLVLLDAGIEFQNYQKTLDLVNE
                     GLREMVMGNFSDEDMEIAKKSVRTSSESIIDSPQLMSEFVFGKIISNDQRSLEEMIQE
                     LEEVTREDIIEAMGKVNLDTIYFMDGLDREVEHENN"
     gene            complement(8283..8930)
                     /gene="deoC"
                     /locus_tag="VFD79_00265"
     CDS             complement(8283..8930)
                     /gene="deoC"
                     /locus_tag="VFD79_00265"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HZH92508.1"
                     /translation="MIDVAGIIDHTALKADTPKDAIRVLCEEAKANKFKAVCVNPTHV
                     KYCSDLLKDTEVRVATVIGFPLGANTPQVKAFETKDAIAMGAHEVDMVINIGALKDGN
                     LDLVRADIEAVVAAADGKALVKVIIETSLLTDDEKAKACQIAMEAGADFVKTSTGFST
                     GGATLEDVRLMRSIVGDKLGVKASGGVKDYKAAIAMMEAGANRIGTSSGVEIAKG"
     gene            complement(8960..9532)
                     /locus_tag="VFD79_00270"
     CDS             complement(8960..9532)
                     /locus_tag="VFD79_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HZH92509.1"
                     /translation="MKKTLPIALSNRHIHISQKDLDTLFGEGYELTKMKDLSQPGQYA
                     CEEKVDVVGPKKTIKGVRILGPVRPDTQLEVSITDAFTLGVSPMIRNSGDVADTPGVR
                     IVGPKGEAEIDRGTIVAARHIHMHTSDGEEFGIKDGEMVSIKVPGPRGLVFDNVLVRV
                     HPSFALEMHVDIEEGNAAGIRNGDMVEVIR"
     gene            complement(9660..10205)
                     /locus_tag="VFD79_00275"
     CDS             complement(9660..10205)
                     /locus_tag="VFD79_00275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HZH92510.1"
                     /translation="MKRKAAKINVRRMVKAGFLVAISIVMTRFAYIMVPLAGVSALRI
                     SFGDLPLMLSGMMFGPLVGGLTGFSADIIGFLINPQGPYHPGFTLSSILWGVIPGLVF
                     YRFRGERDYDKVYSYKNIILAVLLVTVVVSLGLNTYWLSTLYGRGFIILLPTRVLAAL
                     ISIPLHTIILKYLMKYLRRFI"
     regulatory      complement(10263..10355)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            complement(10436..>10626)
                     /locus_tag="VFD79_00280"
     CDS             complement(10436..>10626)
                     /locus_tag="VFD79_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92511.1"
                     /translation="LWILNPRTDLLIQMLPEPFFVDIAGKIGLSLIKYLTLAQVIGYV
                     FYKKGKYTIGRFKSNKRY"
BASE COUNT         2630 a         2656 c         1952 g         3388 t
ORIGIN      
        1 gctcctgtca ttgcaaatga agctacagca acatcattat atccaagtct cagtgatagc
       61 aatccgaatg ccccaaaaac tattgcagag accccgctag caagaccatc cagcccatcc
      121 gttagattga cggcgtttac agttcctatt acgacaacaa gcaaaaatgg tattgttagg
      181 acacccagtg caaggtttgt ctcccccagc aggggtatta ggatctgtgg atatgatcca
      241 gaatgattca aatagtaata tatcaaaacc atggccacca aaaattggcc aatgatcttt
      301 tgataggctc gaagcccaag gttccttttt ttcacaacct ttatatagtc atccataaag
      361 cccactgccc cgaatcccag ggtggagaat attattaccc ccatctgggc attaaggcca
      421 ccagccatta atgtagcagc cacaaaggct atcaggaaaa tgatccctcc cattgttggt
      481 gtgcctgatt tggccaagtg ggacgacggc ccgtcctccc ttatgctttg gcctattttc
      541 agtcgttgca gaagtggtat tatcctaggt cccagaaagg aactcaagac aaagcctgca
      601 ccggttgcca gcagtagctg ggttgattca ttcatatagg tactcctctc aaaatatctt
      661 ccttaatcta tatcattcct attatagtaa agattgactg catttaccag acatatttat
      721 tgacttccga acaagaaaga caacacaagg ttgtctttaa ttggaagtat cattatcctc
      781 gttggtcaag actgggttgc tcctgccatc ctccagatac agatcaatta tggatccctt
      841 gcttacctct accccaggtg aaggatattg atccaatacc aaggtgtcag tattgaaatc
      901 ctcatactct gttgtgtact taagctccag gtcagtaagt tcttttccag cccgattaat
      961 tgagatattc ctgacatcag ggaccaggac tgtttctttt gttgcttcca gctcctcatc
     1021 cgtgtacttt ctgggtacct ccagatagtt gagggtcttt tctattacac ttgctgctgg
     1081 aggacctgca accgtacctc cgtagtagat gccttttggc tcatccacaa taaccaatac
     1141 agctagtctt ggatcgtcaa caggtgccaa gcctccaaat gaagagatat atttcccgtc
     1201 cacatacctt ccgtctatta tcttctgggc tgtaccggtc tttccaccta ttctgtagcc
     1261 aggcacatag gcccttctgc ctgttccctc caggaccgta agctccagca tatccaaaag
     1321 ttcatttgcg gttgcttctg agatcacctg tcttctgatt tcaggttcaa agaggtctac
     1381 aatgtttcca tcagaatcag caaggccttt gactatgtgg ggtgtcataa ggtttccacc
     1441 actgcctata actgaaattg catttaccat ctgaattgga gtgacagcaa tgccgtgtcc
     1501 ataggaaagg gtggcaagat tcacttctct tatgctctca gtccctgatg gaattatgcc
     1561 cctttgttca ccaaggaggt caatgcctgt cacctctcca aagccaaagg ccttgatata
     1621 ttcatacatc ttttcagttc caagttctct ggccatgttt acaaaggcaa cattgcagga
     1681 attgctgaag gccgtagaga agtcctggtc ctgatgggga tcgtaccagc tggagcatct
     1741 aaggacaact cctggaatat cacggataaa tccattgcaa tagtaatggg aatctagatt
     1801 tgccgacccc tcctccaatg ccgcagctgc agttactatc ttaaaggttg aaccaggctc
     1861 ataaatgtca ttgattgcat agtttctcca catctcaaac catttttgct gctggtcctc
     1921 aaatggcagg gcttgccatt ggttgttcag gtcctcatcc agggccatcc tgggattgtt
     1981 ggggtcgtag tcaggcttgt ttgccatggc caagacctct cctgtagcag gatccataac
     2041 gattatggaa acgctttttg cctgattctt ctccagtgcc tcctgagcag cctcctcagc
     2101 aaagccctgt atgatttcgt ctatggtcaa gactacagat gtcccatcct ccggattatg
     2161 gattctttcg cttccgtagg gcagctgcct gttgtccctg tcggtggcct ttacccatat
     2221 tcctggaact cccgacaaat actggtcata caccctttct attccgtaaa ggccattgtt
     2281 gtcaatgtct gtaaagccaa gtatatagga tgcgaaattg ccgaatgggt agtatctctt
     2341 gttgtcgtca acaattgata tccctcctat tccaagttca cgtaccattt ctgcctcttc
     2401 ccttgttatc cattgcttaa gcttgacaac agggctatcc ctccttaggc tttcaagtat
     2461 ttcactgctg tccaccttaa gtattggctg gagtctcatg gctgcctctt caggattatc
     2521 cacatctgaa gggcttgccc atacggtaaa ggaggttaca ttcactgcaa gcttccttcc
     2581 ctttctgtcg tatattattc ccctgtcgga ttttaggttg attgccttgg tccactgttg
     2641 catggcacca cgtttgtatt cagcaccctc gatcacctga acccttacaa gcctgtatcc
     2701 taaaaaaaac acgattaagg tcactaccag tatggcaaac cttaatcgtt tcttggttga
     2761 tttgtttatt tcatgcaatg atatccctcc taataaaggc tggcaaataa accgattaca
     2821 tttctaatct tatcctggaa gctagcctgg taggcatcga tggttactgt ttcattaact
     2881 gtgatatatt caatctggcc ctcttctggg tagatcatcc caaggctgtt tttcgcctgt
     2941 tcaccaatca atgtagtgtt tttcaagcct tccattgttc cagaaagatc ctgcttcaat
     3001 tttgcaagtt cagtggcttc attttccagt ttgcttattt ccaatctcat agaagttatt
     3061 ttagcatatc cttgtagtat aaataaagaa gtacttaata aaataattgc aagggctata
     3121 tacatttgtc tatttagggc atggcttttt ctccgaacta cttttcggac tgttttctgt
     3181 tttttctcac caatgtattc tgggtattga tcatacagtg cttttgccgc catcataaga
     3241 ttgccccctt tacaagtgtc tatattcgtt cagccactct aagctttgcc gacctgcttc
     3301 ttggatttgc atttagctcc tcttcacctg gtacaatagg ttttctggtt ataatctcaa
     3361 tctctcttct cttttggcaa atacaggttg gaaattccgg cggacagatg caatccttgt
     3421 atagatcctt gaattggtcc ttaacaatcc tatcctccag ggaatgaaag gtaattattg
     3481 ccaatcttcc acctggattg aggcattcca ccatttcccc tatggctctt ttcagaacgt
     3541 ccagctcccc gttcacctct atccttattg cctgaaatgt ctttttggct ggatggtgtc
     3601 cctccatcct gaccttcttt ggtatggcct tttttatgac cgtcacaagc tccatggttg
     3661 tgtcgattgg tttttcagac ctttccctga caatgaactc agctattctc tttccccact
     3721 tttcctctcc atactccagg aatattctgg ttaattcctc agaggagtag ttgttgacaa
     3781 catcccatgc tgagaagtcg ctggtggtgt ccatcctcat gtccaagggt gcatccttgt
     3841 tgtgggaaaa tcccctttcc tcggtatcca gctggtggga tgataccccc aggtcaagca
     3901 atattccatc taccttttct atgttcaagt ccttcaatac ttccttcatg ttcacgtagt
     3961 ttgaatggac tagggtaacc ttgtcaatgt gatccttgag tacttccctt gatttttcaa
     4021 gagcattaag atcctggtct attccaacaa gtcttccact tgtgagtctc tttactattt
     4081 ccctggaatg ccctgcccct ccgagagtcc catccacata aatcccatcc tccttgatgg
     4141 ctagcccttc aagtacctca ttcagcaaaa ctgaaacgtg atgaaattcc atgtgaacac
     4201 ccccttaatt tcctgattat attaaatccc aagctctgcc attttctctg cgatgctgtc
     4261 atagcttaga ctatcgtcat cattgtagct atcccattct tccttgctcc atatttcaag
     4321 tcttgtggat acaccaatta ccacggtgtc cttcaacaac ttggagtggt ctcttaggtt
     4381 gcctggtata aggactctcc cctgcttgtc cagtatgcat tcggtggctc ctgaaaagaa
     4441 gaatctgacg aaggctctgg catccttgtt tgtcattggc aggcttttca gcttgtcctc
     4501 gacaattttc cactcatcct ttgggtaaac aaagaggcaa ttgtcaagac ccttggtcat
     4561 tacaaactcg tcaccgagag gatctctgaa ctttgaaggg ataatgagtc ttccctttcc
     4621 atcgatagta tgttgatatt cacctataaa catactcttc tccacctttg ccgtttattt
     4681 accactttcc tccacttcgc cccactttgt atctatttta ccctaaagct cagtttttat
     4741 ccaccatttt agttggataa agtgtggttt tgggacttta gtcctggtgt ttggacaaaa
     4801 caaaaccccg agagtccatc tcccggggtt tgttatagac tgttcagttc tctgatacct
     4861 tcctaaaggc tgccttcaga ggtctttctc ttcctgtgga gaagtgcgcc tccaactact
     4921 ataatcaata cactaataag ctgtgccacc ctaaaggatc caagcataag gctgtcagtt
     4981 ctcaatccct ctatgaagaa ccttgccagt gagtaaagaa ttacatatag gaggaatatc
     5041 tctccctcca ccttggattt ttttcgatac cagatcaaaa acataaatac aagcaggttc
     5101 catattgact cgtacagaaa tgttgggtgg acctttaccc catccaccat tattccccag
     5161 ggaaggtctg ttggccctcc atgggcctcc tggtttgcaa aattacccca tctcccaatg
     5221 gcttggccca gtattagact tggggccgca acatccgcca gggtccagaa tttcagcttt
     5281 tttatcctgg tgaagataat tgcaaccagg gctccaccta ttacagctcc gtggattgcc
     5341 agccctccac ctctgatatt cagtatttgg cctgggttgg agctgtagta gtcccaagag
     5401 aaaacaacgt aataaagtct tgcaaaaaga attgccacag gtactgcgaa aagtagaaag
     5461 tctataaagg tttcctcttt gattcccttt cttcccgctt ccttggtcgc aatcaaagtt
     5521 ccaatcatca ctgctattgc tattagcaat ccataccatc gaacctctag tccgaatatt
     5581 gaaaatgcta ccggatccat tatttaacca cctttcaatt attgcctgat tccatttcag
     5641 gccagattat tgagattaca cttttttcct ctgtgaagtg gctctcgaac cttgtgatga
     5701 tatcctcaaa ggttatttca tttaggacat caaggtaatc tataagcaaa aagtcctgaa
     5761 agtataaatc tgtaaggttg ttggcaataa attctattga attgaaaccc gttaaaaatc
     5821 cccctatttc cttcttcttt attcttgaga aggcttcctg ggtcaaatac ctttcaggct
     5881 tcttaaacat tgccatgatt ctattgtata cttcctgtgg cttgtcgctc tgaccagcta
     5941 tcagggagtg tccgtatgtc ctcttgccag tgtagtatgc tccaaagcta ctgtccacaa
     6001 gaccctccga atacagttca ttgtagaact ccgagctgct ggtaaagagc atgtccagga
     6061 tcatatttgt aataatatcc ttcttgacgg cttccttgcc tgttagaccc aggtctctat
     6121 ccttgacccc gatattgaaa aggggcttgg atgtcatcat tctctcttcg attgacttta
     6181 agaggatttc atccggctca tccgctacta tcctttccac cctatccaag gtagtaggat
     6241 attccttgcc tgcattttcc acggttctta ttatttcatc cccatccagg tcacccacta
     6301 cgaaaagtac catgttacct ggattataga atgtcctgta agctgtgtcc aaatcttgct
     6361 tggtgatttt gtgtatgctc tcaaccgtac ctgcaatatc agttctaacg gaatgtctgt
     6421 ggtacattgc ctttagggtg ttgaaaaaca caacccattt gggattatcc tggtacatct
     6481 ggatttcctg ggcaattatt cctttttcct tttcaacgtt ctcttcagtt agataggggc
     6541 tctgtacaaa tttgacaagg agctcaagat tctggtagaa attgtcggtt gaatagaata
     6601 ggtatgaagt ctgattgaag tttgtatatg catttacata ggctccctgg gatgagaatt
     6661 tttgaaaaat atcttcatca ggttcctcaa acagcttgtg ttcaagaaaa tgagctatcc
     6721 cttcaggtag ttccacaggt tcattttcgc ccaatggaag gaaaacattg tcagtggaac
     6781 cataatttgc agtgaaaatc gcatacttct tggtaaatcc cttctttgga aaataaaaca
     6841 ggtttattcc attttccagt tccttatggt atatttcttc ccttaaattt tcatttctaa
     6901 ttgttttcat gttccacctc cctgtccaat ccatccataa agtagatggt atccaggtta
     6961 accttgccca tggcttctat aatatcttcc cttgtcacct cttcaagctc ttggatcatt
     7021 tcctccaaac ttctctggtc atttgaaatg atctttccaa aaacaaattc cgacataagc
     7081 tgtggactat caataattga ctcactggat gttcggactg atttctttgc aatctccatg
     7141 tcctcatctg aaaagtttcc catgaccatt tcccttaaac cttcattgac aaggtccaat
     7201 gtcttttggt agttctggaa ctcaattcct gcatccaaga gtacaaggga tttgtgcttt
     7261 acaatactag atgaaacata ataggcaagg ctatgctttt cccttacatt cctaaagagt
     7321 attgaattgg gtcctccacc aaagatttca ttccccagga gcaagccatt gtataacttg
     7381 tcctcatagg gtatgcctgc cctgtatccc aataccagct tgccttgggc cactgacatc
     7441 ctatccctga cctggttcct gttctgggat ttttccacta tggtctccct tggtacaaat
     7501 gtaaccgctt ctctgtccat gaactcctct gggatgatcc cttccaaatt caaattatta
     7561 tcgtggccgg tgtaaactat ttcaatctgg ctggtggaaa gaacctttct gtaatggtca
     7621 tagaggatct tcccgtctat ttgagggaca tcctcctggt agccaccggg ataaatgctg
     7681 aatttttcac ccttgcacat ctcttctata catctgttta ttgcataggt cctcttatcg
     7741 ttgatcttat tgttaatcct ggtaatgagg ttattctttt cctgttcaag gtattccctg
     7801 gagaatgaac catcttccat atacgggtca aatattacct ccctgaacat ctgaagaata
     7861 tcactgtcca agccttcttc cccagtgtat cttccatctg cccactccat ggagaacttt
     7921 atgatttgcc tctctcctct tctggatgca ctgattctca ggttggcccc aaaaagttct
     7981 tccagcctcc tcttgagact aagggtgctt ggatggttct tggtccccct ttccaaaacc
     8041 ataggcagca atgagttcaa ggtgacctcc gccctgtcaa gaggtctgat catatatact
     8101 cccaaataat tggatttaaa cttatctgta taggtcaggt ttatattcaa accatttcct
     8161 atttcctttc tcgtggattc catgcctcca atccctcctt cctttgttct acaaaacatt
     8221 tattacccct agaattaatt cctaattatt tatcaaaaaa ataaagggct ttaacccttt
     8281 atttatcctt tagctatttc aacaccagaa ctggttccaa ttctatttgc tccagcctcc
     8341 atcatggcaa tggctgcctt gtagtccttc actccacctg atgccttgac tccaagcttg
     8401 tcccctacaa tggacctcat taaccttaca tcctcaagtg ttgccccgcc tgtgctaaaa
     8461 ccagtggatg tctttacaaa gtctgcacct gcttccattg cgatctggca cgcctttgct
     8521 ttctcatcat ctgtgagaag ggaagtttca ataatcacct ttacaagagc cttcccgtct
     8581 gcagctgcaa caacagcttc aatgtcagcc cttacaaggt caaggttacc atccttcaaa
     8641 gctccgatgt ttattaccat gtctacctca tgggctccca tggcgattgc atccttggtc
     8701 tcgaaggcct tgacctgagg tgtgtttgct cccaaaggaa aacctattac tgtagccact
     8761 cttacctctg tgtcctttaa caggtcactg cagtatttta catgtgttgg attaacacat
     8821 accgctttaa acttgttcgc ctttgcttcc tcacaaagga ctcttattgc atcctttggt
     8881 gtatctgcct ttaaggcagt gtgatcaatt atacctgcta catcaatcat agtctcaccc
     8941 ccaacctagt gtgtttaagc tatctaatca cttccaccat gtcaccgttt ctaattccag
     9001 cggcatttcc ttcctctatg tcaacgtgca tttccaaagc aaagcttggg tgtaccctaa
     9061 caagtacatt gtcgaaaaca aggccccttg gtccaggcac ctttatactg accatttccc
     9121 cgtccttgat cccaaattcc tctccgtcac ttgtatgcat gtgaatatgt cttgcagcta
     9181 caatggttcc cctatctatc tccgcttctc cctttggtcc cacaatccta acccctggtg
     9241 tgtcagccac atctcctgag tttctgatca ttggagaaac gcccagggta aatgcatctg
     9301 taatggatac ctcaagctgg gtgtcaggtc ttacgggtcc aagaatccta actcccttga
     9361 tggttttttt aggtcccaca acatctacct tctcctcgca tgcatattgt cctggctgtg
     9421 acaggtcctt catcttagtc aactcatagc cctcaccgaa aagtgtatcc aggtctttct
     9481 ggctaatgtg aatgtgtctg ttggatagtg ctattggcaa tgtttttttc atcgtaaccc
     9541 tcctaaatta tagttcatat actaattata ctctttttaa caatgttttt caaagagatt
     9601 gtcaaaaaat tactggactt gcaaacactc ccgtcaggtt gacgggagtg gattgatttc
     9661 tagatgaatc ttcttaaata tttcataaga tacttaagga tgattgtgtg tagcgggata
     9721 cttatcagtg ccgccaaaac tcttgtgggc aggaggataa taaagcctct gccgtacagg
     9781 gttgacaacc agtaggtatt aagacccaat gaaaccacaa ctgtaaccag gaggacagcc
     9841 aggattatat tcttgtagct gtacaccttg tcgtaatctc tctctcccct aaacctgtag
     9901 aacacaagac ctgggataac gccccacaaa attgagctca atgtaaatcc cggatgatag
     9961 gggccctgtg ggttgatcag aaagccgatg atatctgccg agaatcccgt taagccacct
    10021 actagtggtc caaacatcat tcctgacagc atcaggggca agtctccaaa gctgatcctt
    10081 aaggctgata cacctgccag gggtaccatg atataggcaa atcttgtcat gacaattgaa
    10141 attgccacca aaaaaccggc cttaaccatt cttcttacgt tgatttttgc tgcttttctt
    10201 ttcatttcaa tctcctttct ttaactgaca gctactgcca attaaagaaa gtatctctaa
    10261 ttgtcagcgg atgcggcaaa gaatcgcaga aaatttcttt cgtcaacggg caacttccca
    10321 tcccgaggca cttaacgctc tttacctact ctgtcttact gaatcaatct aagttctaat
    10381 tatactatat gcaggtatta ttgtaaaccg ttcaataaga acaaggcctg cttgactagt
    10441 atctcttgtt gcttttaaat ctccctatgg tatatttacc cttcttatag aatacatatc
    10501 ctatgacctg ggccagtgtc agatatttta tgaggcttaa tccgattttt cccgctatgt
    10561 ctacaaaaaa tggctctggc agcatttgga taagcaggtc ggttcttggg tttaatatcc
    10621 aaaggt
//
LOCUS       DASRQF010000009         3533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1110762, whole genome shotgun sequence.
ACCESSION   DASRQF010000009 DASRQF010000000
VERSION     DASRQF010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110762"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1312)
                     /locus_tag="VFD79_00285"
     CDS             complement(<1..1312)
                     /locus_tag="VFD79_00285"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZH92512.1"
                     /translation="MRKEYVEYMKDLPINIFLASIDQYPLHWQDAIEVIFVLEGSVRI
                     GIESETYVLDKRELEIINPNEVYRMEGMGENLVLILQIDPGFFERYYEDARDTFYYTN
                     TSDDNAQEEEKYYQLRTLISILVYEAVSKLDDYEDQIEDSLLSLMYHLLNNFHYLFYD
                     EESLKEDEIQLERYHRIMKYLSNNYMDKVSLQDIAKQEFLSSQYLSYKIKEVFGHGFN
                     EYLNQIRVEESTKLLLDTDKSISEISEEVGFSHPRYYNKHFKIHYKLTPLQYRKKNKV
                     SEKQLEKMRKIDYINLKEALPYLETYLSDYERFNFDNRIFKLDIDLKKESDEKALKPT
                     VIDLGDISLLLEEENRRVLREIQSEIGFRYCLVNNLFSDDMDIYRGKNRMFINWTRVE
                     NILEFLEDLRLFPVISTRYVEKYIIEDFKRTFSHIYPDLDKWLEM"
     gene            1410..2084
                     /locus_tag="VFD79_00290"
     CDS             1410..2084
                     /locus_tag="VFD79_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004828308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HZH92513.1"
                     /translation="MEKEKYVPEIKSQLRSRSVVVPEVIYRASGIKILGTRIKSLLFS
                     TDVAIIKNNNANSIMAVYPFTPQLSITHAILEVASVPVFVGVGGGLTSGPRSIQIALQ
                     AELLGAYGVVVNAPTKNDVIREMKSVIDIPIVATVASLEDDYLGKLEAGVSILNISGG
                     PKTSEMVMEIRSKVGKEVPIIATGGATEASILRTIDAGANAITYTPPTSSEIFAEVMN
                     AYRNSY"
     gene            complement(2164..3054)
                     /locus_tag="VFD79_00295"
     CDS             complement(2164..3054)
                     /locus_tag="VFD79_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZH92514.1"
                     /translation="MKDRKVIFTDMALVVVAFVWGSGFVATKSALDHMTPFYIMGSRF
                     FIASTVLALFSIKRLKKATWKDVKAGLVVGILMFFCYMFQTVGLQYTTVGTQAFITAA
                     NIVMVPFFYWALTRKMPSGFEIMAAMVCFVGVGILSLDNNLRIGYGEFLSFLCAVSIA
                     LQIVAVGYYAKNVDVYVLSVVQFSLAAVLSLILAFLLEPPVVQMESGLLSTLLYLGLF
                     STMIPFLIQNIAQRYTTSTHTAIILSLEAVFGSLLGVILLGEGVSTKFLIGCTTIFLA
                     VIASETRFAFLKPKKKETTA"
     gene            complement(3103..>3533)
                     /locus_tag="VFD79_00300"
     CDS             complement(3103..>3533)
                     /locus_tag="VFD79_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92515.1"
                     /translation="NNVAIDNGGVIEEKPLDDKTEGKLTGTYTGVIYAINTIWGSADL
                     YDPVQLVITDNQMVLSNTEPSEIFETSFSFSHTIVQTDINYVAGVGKIPDHYFELLDA
                     GAMYDSQIGCRVTSYSKGKGKLEISYKNEFAGTIYFEVEK"
BASE COUNT          963 a          794 c          729 g         1047 t
ORIGIN      
        1 tcatttcaag ccatttgtcc aggtcagggt atatgtgtga aaatgtcctt ttgaaatcct
       61 caataatgta cttttcaaca tatcttgttg atattactgg aaagagtcta aggtcctcca
      121 aaaattcaag aatgttttca acccgggtcc agtttatgaa catcctgttt ttgcccctgt
      181 atatgtccat atcatctgag aatagattgt tcaccagaca gtatctaaag cctatctcac
      241 tttggatttc tctaagtacc cttctgtttt cctcctcaag gaggagggaa atatctccaa
      301 ggtctatcac tgttggcttt agtgccttct catcagactc cttcttgagg tcaatgtcaa
      361 gcttgaagat cctgttgtca aaattgaacc tttcatagtc agatagatag gtctccagat
      421 agggaagtgc ttccttaaga ttaatgtaat cgatctttct cattttctcc agctgttttt
      481 cagatacctt gttcttcttt ctgtattgga ggggcgtcag cttatagtgg attttaaagt
      541 gcttgttata ataccttgga tgtgaaaatc caacctcctc ggatatttcg ctgatggatt
      601 tgtcagtatc cagaagcaac tttgttgatt cctcaaccct tatctggttc aggtactcat
      661 tgaagccgtg gccaaaaacc tcctttatct tgtaggacaa atactgggag cttaagaact
      721 cctgctttgc aatgtcctga agacttacct tgtccatgta gttgttgctc aggtacttca
      781 ttatcctgtg atacctctct agctgaattt catcctcctt gaggctttcc tcgtcgtaga
      841 ataggtagtg aaagttgttt aggaggtggt acatcaagga aagtagactg tcctcaatct
      901 ggtcctcgta gtcatccagc ttggacaccg cttcatatac aagaattgaa ataagggtcc
      961 tgagctggta gtacttctcc tcctcctggg cattgtcatc tgaggtattg gtgtagtaga
     1021 atgtatccct tgcatcctca tagtacctct caaaaaatcc cgggtctatc tggaggatca
     1081 gcaccaggtt ctcacccata ccctccattc tgtatacctc gttgggattt attatctcaa
     1141 gttccctctt atccagtaca taggtttcac tctcaatccc tatccttact gatccctcca
     1201 ggacaaaaat aacctcgatg gcgtcttgcc agtgaagggg gtattggtct atggaggcaa
     1261 ggaatatgtt tatgggtaag tccttcatgt attcaacgta ttcttttctc atgtgatatt
     1321 cctttcttta ttgtttaaag atgataatgt gtgtgctata atatattgtc aatcagtatt
     1381 agattaccca ttttagatgg aggtgtccca tggaaaagga aaaatatgta ccggaaataa
     1441 aatcacagct aagaagcagg tcagttgttg ttccagaggt tatatacagg gccagcggaa
     1501 taaagatcct agggaccaga atcaagtccc tcctgttttc aactgatgtt gccatcataa
     1561 agaacaacaa tgccaattca ataatggccg tgtacccatt caccccacag ctatcaataa
     1621 cccacgcaat ccttgaggtt gcaagtgtgc ccgttttcgt tggtgttggt ggggggctga
     1681 cctcgggacc gaggtccata cagatagccc tacaggctga actcttgggt gcctatggag
     1741 tggttgtaaa cgctccaacc aagaatgatg tcataaggga aatgaagtct gtaattgaca
     1801 taccaatagt ggccacggtt gcctcccttg aagatgacta tcttggaaag cttgaggctg
     1861 gtgttagcat cctaaatatt tcagggggcc ctaagacctc cgagatggtt atggagatac
     1921 ggtcaaaggt tggcaaggaa gtacccatca ttgcaactgg aggggcaacg gaagccagca
     1981 tcctaaggac catagatgcc ggtgcaaatg ccatcaccta tacaccccct acttcaagcg
     2041 agatatttgc cgaggttatg aatgcatata gaaattccta ttaaaagacc ctgtgaaggg
     2101 tcttttccat tgagacttag aaaaacgagg tccctccgct gacggtcggg atgactgtaa
     2161 aattcaggct gtggtctctt ttttcttggg cttaaggaag gcaaatcttg tctcacttgc
     2221 tatcacagcc agaaatattg ttgtacagcc aatcaaaaac ttggtcgata caccctctcc
     2281 aagaagaatc acaccaagca agcttccaaa tacagcctca aggctaagta ttattgcagt
     2341 gtgggttgat gtggtgtacc tctgggctat gttctgaata aggaagggta tcattgtact
     2401 gaacaagccc aagtaaagca gggttgaaag aagtcctgat tccatctgaa ccacaggagg
     2461 ctccaacagg aaggccaaga tcagtgaaag cacagctgcc agggaaaact ggacaacaga
     2521 cagaacataa acatcaacat tttttgcata gtagcctact gccacaatct gaagtgctat
     2581 tgaaacggca cacaggaagg aaaggaactc accgtatcct attcttaggt tgttgtccag
     2641 gctcagtata ccaacgccta caaagcagac catggctgcc attatctcaa atccactggg
     2701 catttttctt gtgagggccc aatagaagaa gggcaccatg acaatgtttg ctgcggttat
     2761 gaatgcctgg gttccaactg ttgtgtactg aagtcccacg gtctggaaca tatagcagaa
     2821 gaacattaaa attccaacca ccagtcctgc ctttacatcc ttccatgttg cctttttaag
     2881 cctttttatg gaaaacaagg ccaggactgt ggatgcaatg aaaaacctgg agcccattat
     2941 gtaaaatggg gtcatatggt caagggcact ttttgtagca acaaaacccg acccccagac
     3001 aaatgccacc actacaagcg ccatatccgt aaatattact ttcctgtcct tcatgtcaat
     3061 ctcctccgtt gtaaaggtta acctcaagca agctgtcagg acctatttct caacctcaaa
     3121 gtaaattgtt cctgcaaatt cgttcttgta gcttatttcc aactttccct tgccctttga
     3181 gtagctggta actctgcagc ctatttgtga atcatacatt gctcctgcgt ccaatagctc
     3241 aaagtagtgg tcaggtatct tgccaactcc tgcaacataa ttgatatctg tctgaacaat
     3301 cgtatgactg aagctaaagg atgtttcaaa aatttctgaa ggttctgtat ttgataacac
     3361 catctgatta tctgttatta caagctgcac tggatcatac agatcagcgc ttccccatat
     3421 tgtatttatg gcatatatga ccccggtgta ggttccggtc agcttgccct ctgtcttgtc
     3481 atcaagtggt ttttcctcta ttacaccacc attatcaata gctacattat tat
//
LOCUS       DASRQF010000010         2535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1856931, whole genome shotgun sequence.
ACCESSION   DASRQF010000010 DASRQF010000000
VERSION     DASRQF010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1856931"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VFD79_00305"
     CDS             <1..563
                     /locus_tag="VFD79_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HZH92516.1"
                     /translation="GLFSNEAGMGSTPHAHAVAKVKHPGQQGLVAIMGVVIDTGIVCT
                     LTALVILTTGAFETGLSGAALTQMGFVNGLGGFGSYFIAVCLFFFALSTIIGWYFFGE
                     SNVRYLFGKKGLLPYRIIVLACIIIGTTMKVEIVWELADAFNGLMVIPNLIALLGMVS
                     LVVKVTNDFEDNFEKDGPSEYLNKEM"
     gene            657..2015
                     /gene="rlmD"
                     /locus_tag="VFD79_00310"
     CDS             657..2015
                     /gene="rlmD"
                     /locus_tag="VFD79_00310"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HZH92517.1"
                     /translation="MKIKTGDVIEGEIIDFTHEGNGVMKHEGVPIFVEAALIGDVVRT
                     KITGVKKNFATGVVDSMVKTSPDRVEYRFDTQALGGGVPLIGYSYDKQLDWKRSKVEQ
                     DLARIGGLSVQVNPVIGMGHPFRYRNHSQVPVGKVDGKAVTGYYRRKSSEIEPMEEDH
                     LQPEVADQILRTLRSWVDKYEISVYDSDSREGILRHVGIRTNENNESMVILVTTFSQI
                     PSKFQLVHMLVRECPGVVSVYQNINPIDSRFTYGREYIHLFGKEKLTDYIGDLQFEIS
                     PDSFFQINRQQVGVLYGKAREYLEGNKDDIVYDLYSGIGTISLFISGDVKKVIGIESV
                     KAAVDNAKENMKLNNVDNAEFHYGKAEDLFPKMVERGMSANKIILDPPRKGCERELIN
                     AIIELNPERIVYVSCNPSTLARDLKLFVESGYTVEEVQPVDMFPHSAHVECVVLMSRV
                     SK"
     gene            2394..2535
                     /locus_tag="VFD79_00315"
                     /pseudo
     CDS             2394..2535
                     /locus_tag="VFD79_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          755 a          438 c          658 g          684 t
ORIGIN      
        1 ggggactatt ttccaacgag gcaggaatgg gttcaacacc ccatgcccat gcagtcgcca
       61 aggttaagca tcctggccag caggggcttg tggccataat gggagttgtg attgatactg
      121 gtatagtttg taccctgaca gcacttgtga tccttacaac aggagccttt gagactggac
      181 tatccggtgc ggcactgaca cagatgggat ttgtaaatgg actgggtggt tttggctcct
      241 atttcattgc agtatgcctg ttcttctttg cactttcaac aatcatcggt tggtacttct
      301 ttggggaatc taatgtaaga tatctgtttg gcaagaaggg actccttcca tataggatca
      361 tagttcttgc atgtataata attggaacaa ccatgaaggt tgaaatagtt tgggagctgg
      421 ctgatgcctt caacggactt atggttattc ccaaccttat tgcccttctt ggtatggtat
      481 cccttgtggt caaggttacc aacgactttg aggacaactt tgaaaaggat ggaccatctg
      541 aatacctcaa taaggaaatg tagctttgaa agcccggttc tgccgggctt ttgtcatgtg
      601 gacacctatg gaataataat gtagaattaa ggtaggctac agacttggag gttattatga
      661 aaattaaaac tggagatgta atagaagggg aaataataga tttcacacat gagggaaacg
      721 gagtcatgaa gcatgaaggg gtcccaatct ttgttgaagc tgccctcatt ggtgatgtgg
      781 ttaggaccaa aatcacagga gtgaagaaga actttgcaac gggagtggtg gattccatgg
      841 ttaagacctc acctgacagg gtggaataca ggtttgacac acaagctctt ggaggcggag
      901 ttcctcttat tggctactca tatgataagc agctggattg gaaaaggagt aaggtggaac
      961 aggacctggc caggataggg ggattatcgg tccaggttaa tcctgtaatt ggcatgggtc
     1021 atccctttag gtacaggaac cattcccagg ttcccgttgg taaagtggac ggcaaggcgg
     1081 ttacaggata ctatcggaga aaatcctcag aaattgaacc aatggaggaa gaccaccttc
     1141 agccagaggt tgccgaccag atattgagaa cccttagaag ctgggtggac aagtatgaga
     1201 tttcagttta tgacagtgat tcaagagaag gaatacttag acatgtgggt ataaggacaa
     1261 acgaaaacaa tgaatcgatg gtaatacttg tcacgacctt ttctcagata ccaagcaagt
     1321 tccagttggt acacatgctg gtaagggaat gccctggggt cgtaagcgtc tatcagaata
     1381 taaacccaat tgacagcagg tttacctacg gcagggagta catccacctc ttcggaaagg
     1441 aaaagcttac agactatatt ggagacctgc aatttgagat ctccccagac tcctttttcc
     1501 agatcaacag acagcaggtt ggtgtccttt atggtaaggc aagggaatac cttgaaggga
     1561 acaaggatga cattgtatat gacctttata gtgggatagg aacaatatcc ctctttattt
     1621 ctggagatgt aaaaaaggta attggtattg aatctgtaaa ggcggcagtt gataatgcca
     1681 aggaaaacat gaagctcaat aatgtagata atgctgagtt tcattacggg aaggcagaag
     1741 acctattccc aaagatggtg gaaaggggaa tgagtgcaaa caagataatt ctcgatcctc
     1801 caagaaaggg ctgtgagaga gaactgatca atgcgattat tgaactaaac cctgagcgta
     1861 tcgtgtatgt atcctgtaat ccttcaaccc tggcaagaga ccttaagttg tttgtggaat
     1921 caggatacac ggttgaagag gtgcagccgg tggacatgtt cccccattca gctcacgtgg
     1981 agtgcgttgt attgatgtca agagtgagta aataaaaggg ctgtagatgc tcataaataa
     2041 agggtttcca gatattaagt tttcatcgag gctgttttgc cggaggtgct catatcagga
     2101 aacccttatt ttttttgttt aaggtaacat atcagctgcc ctgaaggtga gagggttgag
     2161 ttgacagatt agataacgcc tatttttata ggggttgagg agacaggata gatgtgaatc
     2221 ttgatttgtg ttgaaactgt ctgattgcga ataaaaaata tattcactaa tccttgagga
     2281 tggtatattt acggcgttta aaggattttt attttttctt gagagtaaaa agtaatattt
     2341 tttgggtgtg ttttgaggcg atcacgagaa aatgataaat tatgtcaaag ctaaaagtgg
     2401 taaagcgcat ttatagggag taccttgaag gggcaagctt gctgcaaatc gcacgaggat
     2461 tggaagcgga cggaatactg acggctgcaa acaagaaaaa atggcggcca gagactatta
     2521 agaaaatact gcaga
//
LOCUS       DASRQF010000011         5314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1855796, whole genome shotgun sequence.
ACCESSION   DASRQF010000011 DASRQF010000000
VERSION     DASRQF010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1855796"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VFD79_00320"
     CDS             <1..648
                     /locus_tag="VFD79_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HZH92518.1"
                     /translation="FNALHESNKQIIISSDRPPKEIPTLEDRLRSRFEWGLITDIQAP
                     DLETRIAILRKKANLENMHIPDDVMLLIANKIQSNIRELEGALIRVVAYSSLTNKAIT
                     EELAEEALKDIISNNKPVEITVDLIKDKVSKRFEIRMDDFNSKKRTRAIAYPRQIAMY
                     LTRELTDLSLPKIGDEFGGRDHTTVIHACEKISTDIQSDYQLRNKIDNIIEELGK"
     gene            816..1922
                     /gene="dnaN"
                     /locus_tag="VFD79_00325"
     CDS             816..1922
                     /gene="dnaN"
                     /locus_tag="VFD79_00325"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HZH92519.1"
                     /translation="MKFKVEQKDLARHITIAQRGISTRSTLQILDGILIQANDNGLKL
                     TATDLEISIETFIEASVEEKGKIVLNSRIFGDIVKKLPDDTISFHVKDNHVHIKCQNA
                     EFNIIGNSGEDYPDLPIIMEENQFTISKDLLKSAIRQTVFATTQDETRPSLTGVLLEI
                     SNKEISFVSLDGYRLALRKMPTQSDIDEKIIIPGRSLNELNKILEDKEEDLTISISQG
                     QVIFNLGDTIMYSKLLEGQFFNYKDIMRSDHKTKVVVNRRSLQNGLERASLLAKEEKA
                     NLVKISVTNGELIIKSNSEMGDVYEEINSYQDGDDLNIAFNSRYILEGIKIMDNEEIV
                     MNFVGSLNPCIINGVDDDTYTYLVLPVRLAQDDF"
     gene            1936..2145
                     /gene="yaaA"
                     /locus_tag="VFD79_00330"
     CDS             1936..2145
                     /gene="yaaA"
                     /locus_tag="VFD79_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HZH92520.1"
                     /translation="MEEIRIDTEFIKLDQFLKHCGIAQTGGQAKMMIADGMVKVNGQV
                     ALERGKKIRKDDKVEIDDQYRFLVV"
     gene            2187..3323
                     /gene="recF"
                     /locus_tag="VFD79_00335"
     CDS             2187..3323
                     /gene="recF"
                     /locus_tag="VFD79_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HZH92521.1"
                     /translation="MIVEGIRLINFRNYNNVGVGFSPGINILIGKNAQGKTNLLEAIY
                     MCSTGRSFRTNRDREIINFQKDEAYVGTQLKIGERDKLTEIKLERDKTKRIKVNKIEL
                     KNYRELDTGLKVVLFSPEDLRLIKEGPSNRRNYLDMSISQIKPLYHYNLRRYNKILFQ
                     RNNLLKSFRFKGDLSGLLDVFDMQLAKSGALIMLERDNYLKLLLKEATNIHTIIANGK
                     ETLDIEYNSSVTIEETLSQVETNLLESLRKTRQKDIEVRTTEIGPHRDDFSILINGVD
                     SKTYASQGQQRSIVLTLKLSEVEILKKDTGYYPVLLLDDVYSELDEDRRRYLTELFSR
                     MQTFITTTDAVEINGFERFDKEYFYVQEGCLYKGIKTWREVNGQ"
     gene            3313..5238
                     /gene="gyrB"
                     /locus_tag="VFD79_00340"
     CDS             3313..5238
                     /gene="gyrB"
                     /locus_tag="VFD79_00340"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HZH92522.1"
                     /translation="MANDINVNENGRHYTANEIQVLTGLEPVRKRPGMYIGSTGPKGL
                     HHLVYEVVDNSIDEALAGICDTINVTILEDNSVMIEDNGSGIPVETHPQTGKSTVETV
                     LTILHAGGKFNNSAYKVSGGLHGVGVSVVNALSEWLIARVKRDGKLYEQSFKRGEPDG
                     DIQCIGEADSTGTLIHFKADDEIFETTVFNTDTLEHRFREMAFLNKGIKITLEDRRTG
                     TSKEFHYTGGIKSFVEYINKNKTALHKQVIYFENEKDFTTVELAIQYTDGYTENVFTF
                     ANNIGTAEGGSHLSGLRTALTRCINDYARKYNFLKDKDDNLSGEDIREGMTAILSVKL
                     PEPQFEGQTKGKLGNSETRGIVESLTYEFLNDYLEENPKTSKVILDKAISSKRAREAA
                     RKARDLTRKRSIFDNTTLPGKLADCQSNESSETEIYIVEGDSAGGSAKQGRDKKFQAI
                     LPLRGKIMNVEKARLDKILSFNEIKAMITAFGTGIGEDFDIDKLRYGKIIIMTDADVD
                     GAHIATLLLTFFYRYMRGLVDNGNIYLAQPPLYKVSKGKQEYYCYSDKELDTLLNELG
                     RNSNYNIQRYKGLGEMNAEQLYDTTMDPETRTLIKVNIEDAVAADEIFTTLMGDKVEP
                     RREFIEENAKYVKNLDI"
BASE COUNT         1878 a          929 c         1198 g         1309 t
ORIGIN      
        1 ttcaatgcac tccatgaatc caacaagcag ataattatct caagtgaccg gccacccaag
       61 gaaataccaa cccttgaaga cagacttaga tcaagattcg aatggggtct aataactgac
      121 atacaggctc ctgatcttga aacaaggatt gctatattaa ggaagaaagc caatcttgag
      181 aacatgcaca ttccggatga tgtaatgctt cttattgcaa acaagataca atccaacatc
      241 agggagcttg agggggcact cataagggtg gttgcctact catcccttac aaacaaggca
      301 ataaccgagg aacttgcaga ggaagccctt aaggacatca tctcaaacaa caagcctgtt
      361 gaaattacag tggaccttat aaaggacaag gtttccaaga gatttgaaat cagaatggat
      421 gactttaatt ccaagaaacg gacaagggca attgcatatc ccagacagat tgcaatgtat
      481 ctcaccaggg agcttacaga tctctcactg ccaaagattg gtgatgagtt cggtggcagg
      541 gaccatacaa ctgtaataca tgcctgtgag aaaatatcta ctgatattca atctgattac
      601 cagcttagaa ataaaataga caatataata gaagaacttg gaaagtgatt ttttgtggaa
      661 aagttctaat aattttttaa acttatccac atgttattaa atcccgacaa acaagaggac
      721 aaatgatttg atggacttat ccacataatc acatgtccta ctactattac tactatagtt
      781 tcttattcta ttattctaaa agagggggat aaaaaatgaa attcaaagtg gagcaaaaag
      841 atcttgcaag acatataaca atagctcaaa gaggtatctc gaccagatca acacttcaga
      901 ttctggatgg tattcttatt caggcaaatg acaatggttt gaagcttact gcaacggatc
      961 tggaaataag catagaaacc tttatagagg cttctgtgga ggagaaggga aagatagtac
     1021 ttaactcaag aatttttggg gatattgtaa agaagcttcc agacgataca atcagctttc
     1081 atgtaaagga caaccacgtt catatcaagt gccagaatgc tgagttcaat attattggaa
     1141 actcaggtga ggactaccct gacctaccaa taataatgga ggaaaaccag tttacaattt
     1201 caaaggacct cttaaagtct gcaataagac agactgtttt tgcaacaacc caggatgaga
     1261 caagaccatc actcactgga gtgcttttgg aaatctccaa caaggaaatt tcatttgtat
     1321 cccttgatgg gtacagactt gcacttagaa aaatgccaac ccaatctgat atagatgaga
     1381 agataataat acccggaagg tcactgaacg agttgaacaa aattcttgag gacaaggaag
     1441 aggaccttac aataagcatt tcccagggtc aggtgatatt caaccttgga gatacaataa
     1501 tgtattcaaa gcttcttgag ggacaattct tcaactacaa ggacattatg aggtcggacc
     1561 acaagaccaa ggttgttgta aacagaagaa gccttcaaaa tggtcttgag agggcatcac
     1621 ttctcgccaa ggaggagaag gcaaatctag tgaagatttc agtcacaaac ggagaactga
     1681 tcataaagtc aaattccgag atgggtgatg tatatgagga aatcaactca tatcaggatg
     1741 gagatgactt gaacatagca tttaattcaa ggtatatact tgagggaatc aagataatgg
     1801 acaatgagga aattgttatg aactttgttg gaagcctaaa tccatgcatc ataaatggtg
     1861 tggatgatga tacctacaca tatcttgtgt tgcctgtgag attggcacag gatgattttt
     1921 aagggagtgt ttacgatgga agaaataaga attgatacgg aattcataaa actggaccag
     1981 tttcttaagc attgtggcat tgcacagact ggtggacagg caaaaatgat gattgccgat
     2041 ggaatggtaa aggtcaacgg acaggttgcc ctggagagag gcaaaaagat caggaaggac
     2101 gacaaggttg agatagacga ccagtatcgt tttcttgtgg tataatatat attgttaaaa
     2161 ttcctaagtc ctggggagtg taatcattga ttgtagaagg aataagactg ataaatttta
     2221 gaaattacaa taatgtggga gttggtttca gtcctggcat aaacattctg attggtaaga
     2281 atgcccaggg aaagacaaat ttactggaag caatctacat gtgctctact ggcagatcat
     2341 ttagaaccaa cagggacagg gaaataatca attttcaaaa ggacgaagca tatgtaggaa
     2401 cccagctaaa aataggtgag agggacaagc tcactgaaat aaagcttgaa agggacaaga
     2461 ctaagcggat aaaggtaaac aagattgaac ttaagaacta cagggaactt gacacaggac
     2521 ttaaggttgt cctgttttca ccggaggatt tgaggctcat caaggaagga cccagtaaca
     2581 gaaggaacta ccttgatatg agcatttccc agataaagcc tctttaccac tataacctca
     2641 gaagatataa caagattcta ttccagagga acaatcttct gaaatccttc aggttcaagg
     2701 gagacttgtc aggcctgctt gatgtttttg atatgcagct tgcaaaatca ggggcattga
     2761 tcatgcttga aagggacaat taccttaagc tgcttttaaa ggaagcaaca aatatccaca
     2821 ccattattgc caatggcaag gagaccttgg atattgaata taattcaagc gtaaccattg
     2881 aagaaacctt aagtcaggtg gagaccaacc ttttggaatc tttaagaaaa accagacaga
     2941 aagatattga ggtccgtact actgaaattg gaccccacag ggatgatttc agtattttga
     3001 taaatggagt tgattcaaaa acctatgcat cccagggaca gcagagatca atcgttctga
     3061 cactaaagct gtcagaggtt gaaatactta agaaagatac agggtattac cctgttcttc
     3121 ttcttgatga tgtatattcc gagcttgatg aggacagaag aagatacctt acagaattgt
     3181 tcagcaggat gcagacattt ataacaacaa ctgacgctgt tgagataaat ggttttgaaa
     3241 gatttgataa agagtatttt tatgtacagg aaggatgtct gtataaagga attaaaactt
     3301 ggagggaagt aaatggccaa tgatattaat gtaaatgaaa acggcagaca ttatactgcc
     3361 aatgaaatac aggttcttac ggggcttgaa cctgtaagga agagacctgg aatgtatatt
     3421 ggcagcacgg gacccaaagg actgcatcac cttgtatacg aagtggtgga taacagtata
     3481 gacgaggctc tcgctggtat atgcgacaca ataaacgtaa cgatacttga agacaactct
     3541 gtaatgattg aggacaacgg tagcggtata ccggttgaaa cccatcccca gaccgggaaa
     3601 tcaactgtgg aaacagtact tacaatactt catgcaggtg gaaagttcaa taattctgcc
     3661 tataaggtat cgggaggact tcacggagta ggtgtttctg ttgtaaacgc actgtcagaa
     3721 tggttgattg caagggttaa aagggacggt aaactatacg agcagtcatt taaaagagga
     3781 gagccggatg gagacatcca gtgtattggt gaagcagatt caaccggtac actgattcat
     3841 ttcaaggctg atgatgagat atttgaaacc accgtgttca atacggatac acttgagcat
     3901 aggttcaggg aaatggcctt ccttaacaag ggaataaaga taacccttga ggatagaaga
     3961 actggtacaa gcaaggaatt ccactataca ggcggaatta agtcttttgt tgaatatata
     4021 aacaaaaaca agactgcact tcacaaacaa gtaatttact ttgaaaacga gaaggacttt
     4081 acaacagtgg aactggccat ccaatatacg gacggataca ctgaaaacgt ctttaccttt
     4141 gcaaacaaca taggaactgc agagggagga agccatcttt caggacttag aacagcactt
     4201 acaagatgca tcaatgatta tgcgagaaaa tataatttcc tgaaggacaa ggacgacaat
     4261 ctttccggtg aggatatcag agagggaatg actgcaatcc tttctgtaaa gcttcctgaa
     4321 ccccagtttg aaggacagac caagggaaaa cttggaaata gtgagacaag gggaattgtt
     4381 gaatccctta cctatgaatt tttgaacgac tacctggagg agaatccaaa gacctcaaag
     4441 gtgatccttg ataaggccat aagctcaaaa agagcaaggg aagctgctag aaaggcaaga
     4501 gacctcacta gaaagagaag tattttcgac aatacaaccc ttccaggcaa gcttgctgat
     4561 tgccagtcaa atgagtccag tgaaaccgag atatatatag tcgagggtga ttcagcgggc
     4621 ggaagtgcaa agcagggaag ggataaaaag ttccaggcca tactgccact tcgaggaaaa
     4681 atcatgaatg ttgaaaaggc aagacttgac aagatactaa gcttcaatga gataaaagca
     4741 atgattacag cttttggaac gggaataggc gaggactttg acattgacaa gctaaggtat
     4801 ggaaagataa taatcatgac agacgccgat gttgacggtg cacatattgc tacccttctt
     4861 ctgaccttct tctataggta catgagggga ctggttgaca atggaaacat ctatcttgct
     4921 cagcctccac tatacaaggt atcaaagggc aagcaggagt attactgcta ctctgacaag
     4981 gaactggaca ccctgttaaa tgagcttggt agaaattcca actataatat ccaaagatac
     5041 aagggtcttg gagagatgaa tgcagagcag ctatacgata caaccatgga ccctgaaacc
     5101 aggacactga taaaggtaaa cattgaggat gcagttgctg cagatgagat attcacaacc
     5161 ctaatgggtg acaaggttga accgagacgt gaatttattg aagaaaatgc taagtatgtt
     5221 aagaatcttg acatctagga ggagaatatg gaaaacgaaa acgtaagaga taagttgatt
     5281 gatgtaaata tagaagacaa aatgaaaagc tctt
//
LOCUS       DASRQF010000012         4310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_485039, whole genome shotgun sequence.
ACCESSION   DASRQF010000012 DASRQF010000000
VERSION     DASRQF010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485039"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VFD79_00345"
     CDS             complement(<1..180)
                     /locus_tag="VFD79_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZH92523.1"
                     /translation="MEKYDIAIIGGGPGGYVAAIKAAQKGLNVVLVERERLGGLCLNW
                     GCIPTKTLLISARHYR"
     gene            complement(195..1490)
                     /locus_tag="VFD79_00350"
     CDS             complement(195..1490)
                     /locus_tag="VFD79_00350"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HZH92524.1"
                     /translation="MRFEFQFPDIGEGIHEGVITKWMVQEGDNIEEGESVAEVETDKV
                     TTEIPSPRSGKVLQLKANKGDTINVGDVFIVIDAGGGSEALDEGSKANEKEEVEEETA
                     GVVGQVVSSSDVIPSSTEGSPQTKKTEERRKVLATPVARHLAKDLGVDINTVRGSGPN
                     GRVMKDDIYKASEEKSEPEVKETGLRDRQPKKLSISEDLPVERIPLTKIRKTIAEKMS
                     ESRFTIPHTTAMDEIDISRLHEFRKKYKDSLKEEDVSLTYLPFIIKAAIAALKKYPEF
                     NSSLDEENDELVLKKYFNIGVATDTERGLMVPVIKDADKMNLVEIAKAIEDLSTRAKD
                     NQIQLSQLKGGSFSVTNYGSIGGYFGIPIINYPESAILGIGRVVEKPIVKDGEIVVAH
                     ILPLSLSYDHRIIDGASGARFLNLLKELLSNPDILLLKG"
     gene            complement(1505..2485)
                     /locus_tag="VFD79_00355"
     CDS             complement(1505..2485)
                     /locus_tag="VFD79_00355"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018392213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HZH92525.1"
                     /translation="MANTLSIIQAVNQALINEMKNDDTVIAYGEDVGLEGGVFRATVD
                     LQKTYGKERAFDTPLAESAIVGTAIGMAINGLKPVVEMQFSGFSYPAFNQIISHVARM
                     RNRSRGKYNLPMVIRAPYGGGIRALEHHSESTEALYAQIPGLKVVIPSTPYDAKGLLI
                     AAIRDPDPVIFWEPKRIYRAFKQEVPEEAYEIPLGKAKILQEGEDITIVAWGAMVPDV
                     QKAVKMVQEKGISAEVIDLRTIAPMDRESFIESVKKTGRILVVHEAPKTLGVGAEIIS
                     IVNEKAFLHLEAPPTRLTGFDTTFPLPRGEKHYIPSPERIAKTIEDIVKY"
     gene            complement(2488..3570)
                     /gene="pdhA"
                     /locus_tag="VFD79_00360"
     CDS             complement(2488..3570)
                     /gene="pdhA"
                     /locus_tag="VFD79_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HZH92526.1"
                     /translation="MRLEDYNPMKNEMYQVIDPAGKAKERDHIPDLDEDQLKYLFRTM
                     LYSRTIDEKALSYQRQGRMLTYAPNLGQEAAQVGSAYSLDKEDWLVPTFRELGAWLVR
                     GVPLKNIYLYWYGNEWGSHMPDGVRVLPVSVPIASQLQHATGIGMANNIKKEKNVVLT
                     YVGDGGTSHGDFHEALNFASVFKSPVVFIVQNNQYAISTSRKIQTMSENIALKSTAYG
                     MPGILVDGNDIFAMYNATKAAVDRARKGEGPTLIEAYTYRLGAHTTSDDPTKYRDDAE
                     VEEWRKKDPILRFGNYLKENNVIDDNWIDETKKELEEDVKTTFEAIEDKSDTELEDIF
                     KYHYETMPAQLQEQLDEYRSFLEGGK"
     gene            complement(3713..>4310)
                     /locus_tag="VFD79_00365"
     CDS             complement(3713..>4310)
                     /locus_tag="VFD79_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HZH92527.1"
                     /translation="TVASTMIIAEMAGIKIFATGGIGGVHRGAETTMDVSADLEELSQ
                     TGVAVVCAGAKSILDIGLTLEYLETKGVPVLGYKTDELPAFYTRKSGFSVDYNMGSPK
                     EIADLLRVKWDLELHGGVVVANPIPEEFSMDESFITGIIEKAVVEAKEKGIKGKDTTP
                     FLLAKIKEITGGDSLAANIELVYNNARLAAEIAVELAK"
BASE COUNT          991 a         1103 c          850 g         1366 t
ORIGIN      
        1 tctgtaatgt cttgcggaga taaggagggt tttggtgggt atgcagcccc aattaaggca
       61 tagccctccc agtcgttccc tttccactag aactacattt aaacccttct gggcggcctt
      121 tatggccgcc acatagcctc ctggtcctcc gcctataata gctatatcat atttttccat
      181 catatcactc tccattatcc tttaagaagc agtatgtccg gatttgaaag aagctctttc
      241 agcagattaa ggaatcttgc accgctggct ccatcgatga ttctatggtc ataggatagt
      301 gaaaggggta ggatatgggc aactacaatt tctccatcct tcacgatcgg cttctccaca
      361 acccttccaa taccaagtat ggcagactcg ggatagttta ttatgggtat tccaaagtat
      421 cctccgattg acccgtaatt ggttacggag aaggaccctc ccttaagctg ggacaactga
      481 atctgattat cctttgccct ggtgctcagg tcctcaatgg cctttgcaat ttcaacaagg
      541 ttcattttgt cagcatcctt gatgacggga accataaggc ccctttccgt atctgttgca
      601 acaccaatgt tgaaatactt cttcagtacc agctcatcat tttcttcatc caggctggag
      661 ttgaactcag gatacttctt aagtgctgca atggctgcct ttataataaa tggaagatag
      721 gtcagactga catcctcctc cttaagagaa tccttgtatt tcttcctgaa ttcatgaagt
      781 ctgcttatat caatctcatc cattgcagtg gtatggggga tcgtaaatct tgattcgctc
      841 atcttttctg cgatggtttt tcttatcttg gtcagtggga ttctctcaac aggaagatcc
      901 tctgatatag aaagtttttt aggctgccta tcccttaggc cggtttcctt gacctctggc
      961 tcggattttt cctcgcttgc cttgtatata tcgtccttca tcacacggcc atttggtccg
     1021 cttcctctta cagtatttat atcaacacca aggtcctttg ccagatgtct ggccactggt
     1081 gtggcaagaa ccttccttct ttcttctgtt ttctttgtct gtggtgaacc ttccgtactt
     1141 gaggggatta catctgatga tgaaacaacc tgtcccacca ctcctgcagt ctcctcttca
     1201 acctcttcct tttcattggc ttttgaccct tcatccaacg cctctgatcc tcccccagca
     1261 tcaattacta taaatacatc accgacattg attgtgtctc ccttgttggc cttaagctgg
     1321 agaacctttc cacttctggg tgaaggtatt tctgttgtca ccttatctgt ctcaacctcg
     1381 gcgacagatt cgccttcctc tatattatcc ccttcctgga ccatccattt ggttattacg
     1441 ccctcgtgga ttccctctcc tatatcgggg aactgaaatt caaatctcat ggctgtcctc
     1501 ctttctaata tttcacaata tcctcaattg tctttgcaat tctttcaggg cttgggatat
     1561 agtgtttttc gcctcttggt agtgggaatg ttgtatcgaa acctgtcaac cttgtaggtg
     1621 gagcctcaag atgcaggaat gctttctcat tgacaattga aattatctca gcacccactc
     1681 caagggtctt tggtgcttcg tgaacaacca aaatcctacc ggttttcttt accgattcaa
     1741 taaagctttc cctgtccata ggggcaatgg ttctcaggtc gatcacttca gcagaaatcc
     1801 ccttttcctg gaccatcttg actgcctttt ggacatcggg taccattgca ccccaggcga
     1861 ctatggtaat atcctcaccc tcctgcaata ttttggcttt acccagtggt atttcatatg
     1921 cctcctcagg tacttcctgc ttgaaggccc tgtagattct cttgggttcc caaaatatta
     1981 cagggtcagg atctctaatt gctgctatca atagtccctt tgcatcatat ggggtagagg
     2041 ggattacaac cttgagacct ggaatctgtg catacaatgc ctctgtactt tctgaatgat
     2101 gctccaatgc tctgattcca ccaccgtatg gtgcacggat aaccatgggg agattatact
     2161 tgcccctaga cctgtttctc attctggcaa cgtgacttat gatctggttg aaagccgggt
     2221 aagagaatcc tgaaaactgc atttccacaa caggtttaag accgtttatt gccattccga
     2281 ttgccgtacc cacaatggca gattcagcca gaggagtatc aaaggccctt tccttaccgt
     2341 aggttttctg aaggtcaaca gttgccctga agacaccacc ctctagacct acgtcctcac
     2401 catatgctat aaccgtgtcg tcgttcttca tttcatttat caatgcctga ttcaccgctt
     2461 gtattatgct taaagtattg gccatttcta tttgcctcct tccagaaagg atctatattc
     2521 atccagctgt tcttgaagtt gtgctggcat agtctcgtag tggtacttga aaatatcttc
     2581 aagctctgta tcagacttgt cctcgattgc ttcgaaggtg gtcttgacat cttcctcaag
     2641 ctccttcttt gtctcatcta tccagttatc atcaataaca ttgttttcct taaggtagtt
     2701 tccaaatctc aggataggat ccttcttcct ccattcctca acctcagcgt catctctgta
     2761 cttggtgggg tcgtctgagg ttgtgtgggc gccaagtctg taggtgtagg cctctattag
     2821 agttggcccc tcaccctttc ttgccctgtc aactgcagcc ttggttgcat tgtacattgc
     2881 aaatatatcg tttccatcca ccagtattcc aggcattcca taagctgtag acttcaaggc
     2941 tatgttttca ctcattgtct gaatctttct ggatgtggaa attgcatatt ggttgttttg
     3001 tacaataaag accactggtg atttgaatac cgatgcaaag ttaagggcct catggaagtc
     3061 cccatgggag gttccgccat cacccacata tgttaggaca acgtttttct ccttcttgat
     3121 attgttggcc atacctattc cagttgcgtg ttgcagctga gatgcgatag gcacagaaac
     3181 agggaggacc ctgactccat ccggcatatg actgccccac tcattaccgt accagtaaag
     3241 gtagatgttc tttaggggaa ccccccttac cagccaggcc cccagttctc tgaaagttgg
     3301 aaccaaccag tcctccttat ccaaggagta ggcacttcct acctgggctg cctcctgacc
     3361 cagatttggt gcataggtca gcatccgacc ctgcctctgg taggaaaggg ccttttcatc
     3421 tattgttctg gaatagagca ttgtcctaaa aagatactta agctggtcct catccaggtc
     3481 cgggatatga tccctttcct ttgccttgcc agctggatca ataacctgat acatttcatt
     3541 cttcattgga ttgtaatctt ccaagcgcat gttcaaacct cctaatattt atagtaattg
     3601 ataatgatta tcatctatta ctatatttat atacctaaat gcaaatatta aacattttgc
     3661 ccaccgattt accctaaaaa aaagaactga ggatatcctc agttcaaatt gatcattttg
     3721 ccagctccac ggcgatctct gctgcaagtc ttgcgttatt gtataccagc tcaatgtttg
     3781 cagccagtga atctcctcct gttatctcct tgattttggc cagcaggaag ggagtggtgt
     3841 ccttgccctt tatgcctttt tccttggctt caacaacggc cttttcaatt atgccggtta
     3901 tgaatgactc gtccatggaa aattcttcag ggattggatt ggcaacgaca acaccgccgt
     3961 gaagctccag gtcccatttg acccttagta ggtctgcaat ctctttgggg gagcccatgt
     4021 tgtaatcaac cgagaagcca ctctttctag tgtagaaggc tggtagttca tctgttttgt
     4081 agccaaggac ggggacaccc ttggtctcca ggtattccaa ggtcaaaccg atatcaagta
     4141 ttgactttgc tcctgcacac accactgcaa cacctgtctg tgacaattcc tcaaggtcgg
     4201 ccgaaacatc cattgtggtt tcagcgccac ggtgaacacc accgattcca cctgttgcaa
     4261 atatcttgat tccagccatt tcagcaatta tcatcgtgga tgctacagtt
//
LOCUS       DASRQF010000013         8136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_731817, whole genome shotgun sequence.
ACCESSION   DASRQF010000013 DASRQF010000000
VERSION     DASRQF010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 8136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8136
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731817"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1934)
                     /gene="fusA"
                     /locus_tag="VFD79_00370"
     CDS             complement(<1..1934)
                     /gene="fusA"
                     /locus_tag="VFD79_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019034689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZH92528.1"
                     /translation="MKAYSTEKIRNVALLGHSSNGKTTLTEAMLFATGVTTRQGKVTD
                     GNTMSDFDKQEIARHVSIGTAVIPVEWNNTKFNILDTPGYFDFIGEMYGAKRASEGSV
                     LLVDASSGIEVGTEKAWKNLEKYDTPRIIFVNKMDKENINFDKLLEDLTSQFGDKIVP
                     FAYPNATGEGFNGFANLIDEVAYEYDGKNRKEVPLNDAQKAKIDEYREMFMERIALSE
                     EALMEKYFEGEEFTKEEITRGIRGSVSNGTLVPLVVGCAEKAIGADFLLDLIQRYIPS
                     PADVGSVKGTKDGEVVVRKVDVNEPFSAIVFKTIVDPFVGKLTLFKVVSGKLTRDTEV
                     YNSTKEKAEKLGGLYILRGKNQVDVSEIMAGDIGATSKLAFTQTGDTLCDKNNQTLYE
                     TIKYPQPTLFMAVEPKNKGDEEKIGTSLNKMIDEDPTFVIERNTETKQLLIGGQGNMQ
                     LTVIVDKLKTNFGVDVVLKSPKIAFRETIKGTATVQGKHKKQSGGAGQYGDVHMRFEP
                     CEEEFVFQEEIFGGSVPRNFWPAVEKGVRESLEHGVLAGYPVVNVKATLFDGSYHDVD
                     SNEMAFKIAASIAFKKGMEEAKPILLEPIVKVEVKIPEDYMGDVMGDMNKRRGRILGM
                     EQQADGSQKIIAEAPHTEMF"
     gene            complement(2110..2448)
                     /locus_tag="VFD79_00375"
     CDS             complement(2110..2448)
                     /locus_tag="VFD79_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HZH92529.1"
                     /translation="MRKIAFFAFRGDEMCFMHLVLNALDLENKGYQVKIIMEGEAVTL
                     MEKLEKGENKPYLKAKEKGLFDCICKACSAKLGVLEFNQTVGIPLKGNLEGHPAMEDY
                     IKEGYEIITL"
     gene            complement(2473..3075)
                     /locus_tag="VFD79_00380"
     CDS             complement(2473..3075)
                     /locus_tag="VFD79_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92530.1"
                     /translation="MEYFRYGDEEKDYLAGRDPIMKVLIQKHGHIEREINPDPFSALV
                     GSIISQQISTKAAETVEGRLVHRAGGLTAKNLHNLSLEEIKECGLSFRKAGYIKGITE
                     AAISGAVSFEVLKNLSDEEVIKSLVGLKGIGRWTAEMMLIFSLARRDVLSLDDLGIRR
                     GIQRAYGLEEVKKDQHSYFRDLFSPYGTVASFYLWEEASN"
     gene            3155..3475
                     /locus_tag="VFD79_00385"
     CDS             3155..3475
                     /locus_tag="VFD79_00385"
                     /inference="COORDINATES: protein motif:HMM:NF023184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HZH92531.1"
                     /translation="MDANTSSKKITREKEIVSLMIDLYFKGSKNSLAYGEYMDLRDYC
                     LKKLDSCAYGEGKPFCSSCKIHCYDEVHREKIRSVMGYSAPRMLFYHPIMMIRHTLTS
                     LKKS"
     gene            complement(3472..4662)
                     /locus_tag="VFD79_00390"
     CDS             complement(3472..4662)
                     /locus_tag="VFD79_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZH92532.1"
                     /translation="MDPGGERQGMKIEWNVRYLTIAAYALVVTCLSILFYLIASDFTI
                     FSEKVSWFLGALQPIIIGAVMAYLFNFILEMIEKYILDPVITKGKIPRLRRTIGMLLT
                     YAVIGTLIYLFAIIIIPQLIASVARLVTLVTDNFDSISLWVTDQIQRLHIREDYLQYI
                     VDWWNGVLDNALGFVTGLIPVIGSIVGVILASIWNIAIGLVVSVYLLFDKDRFRALGR
                     KVVYSIFHGPRANRTLELARRADTIFGRFLSGKILDSFIIGILTYIILLIFKMPFPIL
                     VSFIIGVTNIIPFFGPFIGAVPGVFIIAIVSPIQALWFIVIVILLQQFDGNILGPKIL
                     GDSLGISAFWILFSLLIAGKLLGFVGLIIGVPLFVFIYSIIKDNVERRLAKKGLPIET
                     REYF"
     gene            complement(4632..5612)
                     /locus_tag="VFD79_00395"
     CDS             complement(4632..5612)
                     /locus_tag="VFD79_00395"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HZH92533.1"
                     /translation="MKMPERIRLANLPTKIEKLERLSQELGRNIWIKRDDQTGMEVSG
                     NKVRKLEFAVMEALELGCNHLITCGGIQSNHARATAAVAAKLGMGCTLVLRGEDREDR
                     EGNLFLETLMGAQVVFVTPEEFEESLDGIMEDIRARLAQEGKKGYILPVGASNGIGSF
                     GYLNAYNEIVEQERMLGLKFDAIIATVGSGGTFAGLYAGNSLDGSQRDVIGINISSTA
                     DYFKERVVALLQEMEEYTDLAFETDERDIQIIDGYPGLGYAMSRDEELHFIKWLARLE
                     GLVLDPVYTGKAMYGLVKEIEAGNLDDYENILFIHTGGIFGWTREVRDRV"
     gene            complement(5632..6240)
                     /gene="lepB"
                     /locus_tag="VFD79_00400"
     CDS             complement(5632..6240)
                     /gene="lepB"
                     /locus_tag="VFD79_00400"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZH92534.1"
                     /translation="MGNGKSIVGEIFSWIVSLGVALLVALFIISNVVSMTMVMEQSME
                     PTFSQGDRLIVNRVGYFFTGPNRGDVIIFDKNPIEKGIIRNMINEISDIRNSLRFRLT
                     GSAEKNLLIKRVIAVGGDQVELIDGEVFVNGERLQEPYIDKMTYSRAETSWSVPEDKI
                     FVLGDNRENSLDSRSIGFVDIQQVKGKVAFRIFPFSEFGSID"
     gene            complement(6259..6627)
                     /locus_tag="VFD79_00405"
     CDS             complement(6259..6627)
                     /locus_tag="VFD79_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HZH92535.1"
                     /translation="MSSFNFIFMLFPVFFLLIFAVIAVTLIKGLVGWKKNNDQPILNV
                     NATVVSKRENFRRSANNHNNHAMRTGHTSYFVTFQFDSGDRLELQVRGEEYGMMVEGD
                     GGVLVFQGTRFHSFDRFTDL"
     gene            complement(6644..7249)
                     /locus_tag="VFD79_00410"
     CDS             complement(6644..7249)
                     /locus_tag="VFD79_00410"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZH92536.1"
                     /translation="MRLFITRHGQTQWNTEGRLQGWMNSPLTPKGLKGAEQLRDAVRN
                     IDFDGVFTSDQYRAVKTAEIVVGDRDIEINKIMELREIAFGQWEGMKLVDIKEKYGKE
                     FHNYMNSPMEYRSYGGESIDELFDRVEKGLQIVVDSGAQNALIVAHGVTIKALVTILK
                     GMPRASISKLPVFPGTSLSIFNRVGDFWQVELEGDTSHFHL"
     gene            complement(7265..7768)
                     /locus_tag="VFD79_00415"
     CDS             complement(7265..7768)
                     /locus_tag="VFD79_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009430122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZH92537.1"
                     /translation="MDRIETRIEDVTLRLAKEEDLSLILRFIKELADYEKLLHEVVAT
                     EEILRESLFERRAAEVVIAEYQGEPVGFALFFHNFSTFLGRPGLYLEDLYVRPEMRGK
                     GIGKLLLSYLAKLALDRGCGRFEWWCLDWNKSSIDFYKSIGAIPMDEWTVYRVCDKAL
                     EDLAEEF"
     gene            7913..>8136
                     /locus_tag="VFD79_00420"
     CDS             7913..>8136
                     /locus_tag="VFD79_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HZH92538.1"
                     /translation="MTDLSNKKCTPCEVGAIPLQPMEIKNMLKDLEEGWTSEDDKSIQ
                     KTFKFDDFMGAMDFANRIADLAEEEGHHPTL"
BASE COUNT         2140 a         2128 c         1473 g         2395 t
ORIGIN      
        1 tcaaacatct ccgtgtgagg tgcctctgca atgattttct ggcttccgtc agcctgctgc
       61 tccataccga ggatcctgcc cctacgcttg ttcatgtctc ccattacatc gcccatgtag
      121 tcctcaggta tcttgacctc aacctttacg attggctcta gaagaatcgg cttcgcctct
      181 tccataccct tcttgaaggc aatggatgcc gctatcttaa atgccatctc gtttgagtca
      241 acatcgtggt aggaaccatc aaacaaggta gccttgacgt taactacagg ataacctgct
      301 agaacaccat gctccaaaga ttccctgact cccttttcaa ctgctggcca gaagtttctt
      361 ggaacagacc caccaaagat ctcctcctgg aatacaaact cctcttcgca aggctcaaac
      421 ctcatgtgaa catctccgta ctgacctgct ccacctgatt gtttcttgtg cttgccctga
      481 actgtggcag ttcctttaat ggtctccctg aatgcaatct ttggactctt aagtacaaca
      541 tccaccccaa agtttgtctt cagcttgtct acaataacgg taagctgcat gttaccctgt
      601 ccaccgatca ggagctgctt ggtttctgtg tttctctcta ttacgaatgt tgggtcttca
      661 tctatcatct tgttaaggga tgtaccaatt ttctcctcat ctcccttgtt ctttggttca
      721 actgccatga acaatgttgg ttgaggatac tttattgtct cataaagtgt ctggttgttc
      781 ttgtcgcaaa gggtgtcacc ggtttgtgta aaggccaact ttgaggttgc tccaatatcc
      841 cctgccatta tctcggagac atcaacctgg ttcttacctc taagaatgta aagcccaccc
      901 agtttctcag ccttctcctt ggttgagttg tagacctccg tatcccttgt cagcttgccg
      961 gaaacaacct tgaaaagggt aagctttcca acaaatgggt cgactatggt cttgaagaca
     1021 attgctgaga aaggttcatt cacatcaacc tttctgacaa ctacctctcc atccttggtt
     1081 ccctttacgc ttccaacatc tgctggagat gggatgtatc tttggataag gtcaagcagg
     1141 aagtcagctc caattgcctt ttcagcacag cctacaacaa gtggtaccag ggttccgttt
     1201 gaaacgcttc cccttattcc ccttgttatc tcctccttgg taaattcctc accctcgaaa
     1261 tacttctcca tcaatgcttc ttcggaaagt gctatcctct ccataaacat ctctctatat
     1321 tcatcaatct ttgccttttg ggcatcgttt agaggaactt cttttctgtt ttttccatcg
     1381 tattcataag caacttcatc tataagattt gcaaagccat tgaaaccctc tcctgttgca
     1441 ttggggtatg cgaaaggaac tatcttgtct ccaaactggc ttgtcagatc ctcaagcaac
     1501 ttatcaaagt tgatattttc cttatccatc ttgttaacaa atattattct tggggtgtca
     1561 tacttctcaa ggtttttcca tgccttctca gttcccacct ctattccaga ggatgcatca
     1621 acaagaagca ctgagccttc actggccctt ttcgctccat acatttcacc gataaagtca
     1681 aaatatcctg gtgtgtctag aatattgaat ttggtattgt tccattccac cgggataact
     1741 gctgttccaa ttgatacgtg tcttgcaatc tcctgcttgt caaagtcgga cattgtattt
     1801 ccgtctgtta cctttccctg tcttgtagtc acacctgttg caaatagcat tgcttctgtt
     1861 aaagtggttt ttccattact actgtgccca agaagggcta catttctgat cttttccgtt
     1921 gaatacgctt tcaagataca ttccccctaa ctagaatttt aaagatatta taaccttttc
     1981 aattactgca attttcaaac aagtcttaat tctacaccta ttcattataa catgaaacat
     2041 ttgttttatc aaaatatatt taagcgtgaa aaagcacccc aaaggggcgc tttatggcgt
     2101 tttcttcatt taaagtgtga tgatttcgta gccttccttt atgtagtcct ccatggcagg
     2161 atgaccctca agatttcctt ttaagggaat ccccacagtc tggttgaact caagaacacc
     2221 aagttttgca gagcaggcct tgcagataca gtcaaacagc cccttctcct tggccttgag
     2281 gtatggcttg ttctccccct tctcaagttt ctccataagg gttacagcct ccccctccat
     2341 gatgatcttc acttggtatc ccttattctc aaggtccaag gcgttaagta ccaggtgcat
     2401 aaaacacatc tcatcgcctc tgaaggcaaa aaatgctatc tttctcatgc taagtcctcc
     2461 tttcaaataa ccctaattag atgcttcctc ccacagataa aaggatgcca ctgttccgta
     2521 tggactgaaa aggtccctga agtatgaatg ctggtctttt ttgacctcct ccaaaccata
     2581 ggccctttga atcccccttc ttattcccaa gtcgtccagg ctgagaacat ccctcctggc
     2641 aagggagaaa atcagcatca tttctgctgt ccatcttcca atacccttaa ggccaacaag
     2701 actcttgata acctcctcat cagaaaggtt tttcaggact tcaaaactta cagctccgct
     2761 tattgcagcc tcagttatgc ccttgatata cccggctttc ctgaaggata atccacattc
     2821 cttgatctcc tcaaggctaa gattatgaag atttttggct gtcagcccac ctgcccgatg
     2881 aacaagcctt ccctccacag tctctgcagc cttcgttgaa atctgttggc ttattatgct
     2941 tccaacaagg gcggaaaagg ggtctgggtt tatctccctt tctatatgcc catgcttttg
     3001 aatcaagacc ttcatgatag gatctctccc tgcaagataa tccttttctt catccccata
     3061 cctgaaatat tccattactt gctccttctc tattcaattt gccttgctaa aatgctataa
     3121 taatcatata tccacaacaa aggagacggc caccatggat gcaaacactt cctctaagaa
     3181 aatcacaaga gaaaaagaaa tcgtatccct aatgattgac ctttacttta agggcagcaa
     3241 gaactcactt gcatatgggg agtacatgga tcttagggac tactgcctaa aaaaacttga
     3301 ctcctgcgcc tatggtgaag gcaagccctt ctgctcctcc tgcaaaatcc actgctatga
     3361 tgaggtccac agggaaaaga ttcggtctgt catggggtac tccgcaccca ggatgctctt
     3421 ttatcatccc attatgatga taagacacac cctgacctcc ctaaaaaaat cctagaagta
     3481 ttcccttgtc tctattggaa ggcctttctt tgccaatctt ctttcaacat tgtccttaat
     3541 gattgagtag atgaacacaa atagaggtac tccaattatc agccccacaa agccaagaag
     3601 ctttcctgca atcagcaatg agaatagaat ccagaaggct gatatgccca gggaatcccc
     3661 aaggatcttt ggcccaagta tgttgccgtc aaactgctga agcagaatta ctataactat
     3721 aaaccacagg gcttgaatcg gtgagacaat tgcaataatg aagactccag gcactgctcc
     3781 gataaatggt ccaaagaagg ggatgatatt tgtaaccccg atgataaatg atacaaggat
     3841 cgggaaaggc atcttgaata tcaatagtat gatataggtc agaatgccaa tgataaagga
     3901 atccaaaatc tttccactaa ggaatcttcc gaaaatcgta tctgcccttc ttgccagctc
     3961 cagtgtcctg tttgccctgg ggccatggaa aatggagtag accacctttc tacctagtgc
     4021 cctgaacctg tccttgtcaa agagcaggta aacagacacc acaagaccaa ttgctatgtt
     4081 ccagatgctt gccaggatca caccaacaat gctcccaata actggaatca aacccgtgac
     4141 aaatcccaat gcgttgtcga gaacaccatt ccaccaatcc acaatatatt gaaggtaatc
     4201 ctcccttata tggagtcttt ggatctggtc agtgacccaa agggatatac tgtcaaaatt
     4261 atctgtgaca agggtgacaa gccttgctac ggatgctatt agctgtggaa tgataatgat
     4321 ggcgaacagg taaataaggg ttccaattac agcataggtt aaaagcattc caattgtacg
     4381 cctcagtctt ggtatctttc cctttgtgat gaccgggtca aggatgtact tctcaatcat
     4441 ctccagaata aagttaaaca ggtatgccat tacagcaccg attattatgg gctggagggc
     4501 gcctaggaac caggagacct tttcgctgaa tattgtaaag tcagatgcga tcagataaaa
     4561 taggatactc agacatgtga ccaccagggc atatgcagct attgtaagat atctaacatt
     4621 ccactcaatc ttcataccct gtctctcacc tcccgggtcc atccaaaaat tcctcctgta
     4681 tgtataaaga ggatattttc ataatcatcc aggtttcctg cctctatctc cttgaccaag
     4741 ccatacatgg ccttgcctgt gtagacaggg tccagaacaa ggccttccaa ccttgcaagc
     4801 catttgatga aatggagctc ctcatccctg ctcatcgcat agccaagacc aggataccca
     4861 tcaattatct gtatgtccct ttcatctgtt tcaaatgcaa ggtctgtgta ttcttccatt
     4921 tcctggagaa gggccactac cctttcctta aagtagtcag cagttgagga aatgtttatt
     4981 ccaataacat ccctctgact tccatccaga gagtttccgg cgtagaggcc tgcaaatgtt
     5041 cctcccgagc caaccgttgc aataatggca tcaaacttaa ggcccagcat cctttcctgt
     5101 tcaactattt cattatatgc atttaggtag ccgaaggacc caattccatt tgatgcccct
     5161 acaggcagga tatatccttt tttgccttcc tgggctagcc tggcccttat gtcctccata
     5221 attccgtcaa gggactcctc aaactcctca ggggttacaa atacaacctg ggcacccata
     5281 agggtttcaa ggaatagatt tccttcacgg tcctccctat cctcacctct aaggactagt
     5341 gtacacccca ttccaagctt tgctgcaaca gctgcagtgg ccctggcgtg atttgactgt
     5401 attcccccac aggtgatcag atgattgcat ccaagttcaa gagcttccat aacagcaaac
     5461 tcaagctttc tcaccttgtt tccagatacc tccatcccgg tctggtcatc cctttttatc
     5521 cagatgttcc tgcccagctc ctggctaagt ctctcaagct tttcaatttt tgtaggtaaa
     5581 tttgccaatc tgattctttc aggcattttc ataaccatac ctcctggatt tttaatctat
     5641 tgacccaaac tcgctgaatg gaaatattct aaaagcaacc ttacccttaa cctgctggat
     5701 gtcaacaaat cctatgcttc ggctgtctag gctgttctcc ctgttgtcgc caagtacaaa
     5761 tatcttgtct tccggaacgg accagctggt ctctgccctt gaataggtca ttttgtcaat
     5821 gtagggctcc tggagccttt caccgttgac aaagacctct ccatcaataa gctcaacctg
     5881 gtctccaccc actgcaataa ctctctttat gagaaggttc ttctcagcac tgccagtcag
     5941 tctaaacctc aggctgtttc tgatgtcgct gatttcgttt atcatatttc ttatgattcc
     6001 cttttctatg gggttcttgt caaatattat cacatccccc ctattaggac ctgtgaagaa
     6061 gtaaccgacc ctgtttacaa taagcctgtc tccctggctg aaggttggtt ccatggattg
     6121 ttccatgacc attgtcattg aaactacgtt cgaaataata aagagtgcaa ccagaagcgc
     6181 aactccaagg gacacgatcc aactgaaaat ctcccccact atacttttac cattacccat
     6241 ttgattcatt cccttcagtt ataagtcagt aaacctgtca aaactgtgga atctggttcc
     6301 ttgaaaaacc aaaaccccac catcgccttc aaccatcatt ccatactcct ctccccttac
     6361 ctggagctca agcctgtctc cactgtcaaa ctgaaaggtg acaaaatagc tggtgtgacc
     6421 ggttctcatg gcatgattgt tatggttgtt tgcagacctt ctgaaatttt ctcgctttga
     6481 tacaactgtt gcgtttacat ttagaatcgg ttgatcgttg ttctttttcc atcccaccag
     6541 gcctttaatc aaggtgactg cgattacagc gaaaatcagc aggaaaaata caggaaaaag
     6601 cataaatata aaattaaagc tgctcattgt atacctccat taattacagg tgaaaatgtg
     6661 acgtgtcccc ttcaagctcc acttgccaga aatctcctac cctattgaat atgctaagtg
     6721 atgtccccgg gaaaaccgga agcttcgaaa tggatgccct tggcatcccc ttaagtattg
     6781 tcacaagtgc ctttatagtg actccatgag ccacaatcag agcattttgt gcccccgagt
     6841 caaccactat ctgaaggccc ttttcaaccc tgtcaaaaag ctcatcgatg gattccccac
     6901 cataggacct gtactccatg gggctgttca tgtaattgtg gaattcctta ccatacttct
     6961 cctttatgtc cacaagcttc atgccttccc attgtccaaa tgcaatctcc ctgagctcca
     7021 taatcttgtt aatctcaata tccctatctc caactacaat ctccgcggtc ttgaccgccc
     7081 tatattggtc gctggtaaaa accccgtcaa aatctatatt ccttactgca tccctaagct
     7141 gttcagcccc cttaagtccc tttggtgtca atggtgagtt catccagccc tgtagcctac
     7201 cctcagtgtt ccactgggtc tgtccatgtc ttgttataaa taatctcaac tcatttcctc
     7261 cctgctaaaa ttcctctgcc aggtcctcaa gggccttgtc gcaaactctg taaaccgtcc
     7321 actcgtccat tgggatggct ccgatgctct tgtaaaagtc tatggaagac ttgttccagt
     7381 caagacacca ccactcaaac cttccacagc ccctatcaag agccagtttt gccaggtatg
     7441 aaagaagtag cttgcctatg ccctttcccc tcatttcagg tcttacatac aggtcctcaa
     7501 gatagagtcc cggtctacca aggaaggttg agaagttatg gaagaacagg gcaaagccta
     7561 caggctctcc ctggtattct gcaatgacaa cctcagcagc ccttctttca aaaaggcttt
     7621 cccttaaaat ctcctccgtt gcaactacct catgaaggag cttctcgtag tcagccagct
     7681 cctttataaa tctaagtatc aaggaaaggt cctcttcctt tgccaatctc agtgttacat
     7741 cttcaattct cgtttctatt ctgtccatca agccatcccc tctcaaataa tactcaatta
     7801 agtagattat atcacaagga cgaccttaat tggcagagta aataaacact atttcaataa
     7861 ttcagaccat ttctgggtaa ctatggatta gatgacaaag gaggtgttca atttgaccga
     7921 tctttcaaat aagaaatgta ccccctgcga ggttggggca ataccccttc agccgatgga
     7981 gataaagaac atgcttaagg accttgaaga ggggtggacc agtgaggatg acaagtccat
     8041 ccagaaaacc ttcaagtttg atgactttat gggggcaatg gactttgcaa acaggattgc
     8101 agaccttgca gaggaggagg gccatcaccc aaccct
//
LOCUS       DASRQF010000014         5519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_731432, whole genome shotgun sequence.
ACCESSION   DASRQF010000014 DASRQF010000000
VERSION     DASRQF010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731432"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VFD79_00425"
     CDS             complement(<1..740)
                     /locus_tag="VFD79_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HZH92539.1"
                     /translation="MDNIYEIVKDINSILWGPVMLFLLLGTGVLFTFKLRFIQIRKLK
                     TVFKSTFADIFKKNDKANEDGMSSFQALSTAIAAQVGTGNLAGVATAIAAGGPGAIFW
                     MWVSGFFGMSTIFAEAILAQVHRKRVGGVIVGGPAYYIRDGLNNKFLAGFFSLAIILA
                     LGFMGNMVQSNSISEAITGAINVPPILVGVVVAAIVGLIVIGGIGRIASFTEKVVPFM
                     AALYILGSVSILVLNADSIIPALRMIIVG"
     gene            complement(1088..1903)
                     /locus_tag="VFD79_00430"
     CDS             complement(1088..1903)
                     /locus_tag="VFD79_00430"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HZH92540.1"
                     /translation="MKETLTLQFLGQVYQLKSYSLMMWIAAIAVIIITLVNIRRSGLS
                     IRSSLLPLAIMTLSVPIGARVLNVLINPSYYEKYPKKIFAINNSGFSLMGGLLMAAAI
                     GWLISRLVGISPWKLGDNLASGLGVGLILMRMGCLLNGCCFGLPTDLPWAISYPFGSI
                     AHKHYISIADQGSISLFALFSSPDLHPTQVYEMIGAMVAVWASYMIIKRDYPEGSAIL
                     AASGIFTLTRLINHFFRVHPETNAVPFLFYPIIYIAILGFIGYMIVKRIRIKV"
     gene            complement(1924..4026)
                     /locus_tag="VFD79_00435"
     CDS             complement(1924..4026)
                     /locus_tag="VFD79_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92541.1"
                     /translation="MFKVKGINGILAIVIAASMLLGGCGGTESSTGGPEVKASEPTNW
                     EYTKDIPDPLTATSGAEDVVLGDLEVHGVMVHIPGGTFDTPTEVSLVNPDSAPGYDEK
                     EMNGLGAPIQITAGDGPVRLMQPVTITMKYNPDEVGDDFETGAMYFGYFNESEWEYIK
                     ADVDTQAQTMTFTTSHFSLFSKAKLTVDERIDQYVDNSAVADWLKDQTSTMTEDALNS
                     AIEHILIDNLKINNKSMIAQIYNSVLEDDEWKSMVESVNAGDPAMFNQNLQVLLGKKI
                     VENVSESTLSNALKSLTGDLGIETVEKASEAAGYVAEGRYRDAAKIIGEHIADQFMIT
                     KVGKFAVAAIEGQIQSWKSEEVEAAYQAFKNGASSNVPWWGYQVEKENFEDVWSQMGG
                     AARQLELEAVRAQNEARKDAGMEPLTREEEDKIRACVKKDLEKEFKSRIQKEAEIDKK
                     KEELNMILEMYKDAGLMNKGAFNWTESYELETRMDILMNLKDKVLRDTGRKFIKSGGV
                     HNEEAIAVEQLRMIALIWFGTDDEEQRRKEYAEYLLNELGVSIYAEANELNGIWSSAA
                     LIITEFDLGPPPEPSESSDESDVEGCDLMDIDVYSMVKAALEERIGQPLAVGMKLQID
                     ENGSGTLSIIGEDGDNTDIPATYNNGILSAVGIIEGTGINMEGYVSESNGAISLSGSF
                     IIDMSEDAWIKGTWTGNK"
     gene            complement(4152..5501)
                     /locus_tag="VFD79_00440"
     CDS             complement(4152..5501)
                     /locus_tag="VFD79_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZH92542.1"
                     /translation="MKFLKDKEFLKGMLAIAVPVSLQNLISSSLNMIDTLMISSLGQS
                     SIAAVGLANQIFFLYILLTFGINSGSSIFIAQFWGRDDVLSIKKVLGLAITISTIAGT
                     IFTFIALVLPEFVMGLFIEEQEVIQLGASYLRIVGLSYIITAISFAFSIALRTTGRPG
                     VPLKVSIISFTVNTALNYVLIFGKLGMPAMGIRGAALGTLIARIVEILLIVYAIYGTK
                     SILAASFSELMSWKKDFVKRYIDTIYPVILTEGFWALGQVMYTAAYARIGEEATAAVQ
                     LTNTIQNVFFVIVRGLANACGVMVGSKVGAGEEDKAYEYAISFIIIATISGLVLGIVM
                     ALGPDIPLSLFRGLDPSLYAVSRRLLVFMGLTFVIRVYNTIAIVGVLRAGGETKVAMK
                     IDLSTVWLIGVPLAFIGALVLKLPVEYVFLLVTMEEVVKAFLGIPIIKSGKWIKNLT"
BASE COUNT         1439 a         1358 c         1003 g         1719 t
ORIGIN      
        1 ccgacgatga tcatccgaag ggctgggatg atgctgtcgg cattcaggac gagtattgaa
       61 acgcttccaa gtatgtaaag ggctgccata aagggtacaa ccttctcagt aaagcttgca
      121 atcctaccaa ttccacctat cactataagg ccgactattg cagcaacaac aaccccaacc
      181 agtattggcg gtacgttgat tgcccctgtg atggcctcac tgattgaatt ggactgtacc
      241 atattaccca taaatccaag tgcaagaata attgccagtg agaagaagcc tgcaagaaac
      301 ttgttgttga gaccgtccct tatataatat gccggccctc caacaataac accacctacc
      361 ctttttctgt ggacctgcgc aagtattgcc tctgcaaata ttgtactcat gccaaagaac
      421 ccacttaccc acatccagaa tattgcacct gggcctcccg cggcaattgc cgttgcaaca
      481 cctgccaggt tacctgtacc gacctgggct gctatggctg ttgacaatgc ctggaatgat
      541 gacatcccat cctcattggc cttgtcattt ttcttgaaaa tatctgcgaa tgtagatttg
      601 aatactgtct ttagtttcct gatctgaata aacctaagct taaatgtaaa caatacacca
      661 gtcccaagta atagaaatag cattaccggt ccccacaaaa tactgttgat gtctttgact
      721 atttcataaa tattgtccat gtcttctcct ccgtttgacc accgatgata ttgatattgt
      781 tttaactttt tatgcatgtt ttatttcata tttatatgta ttattgcatt tttagtttta
      841 tttcatattc ttctgttatt cgtgtttatt gttttctaat gtttatttta acttattcat
      901 atctcctcct tcgaataatt tcttctcatg cttatgcaat tttttcatcg gtgatattgt
      961 tattatttta tcattatatc caatgtaaca ccttttacat tttctttcaa cccctaattg
     1021 tagttatttt tcaaaaaaaa caaaaagact gtaaaatatg aatacagtct ttttcttatg
     1081 tcttcaatta cacttttatg cgtatccttt tcacaatcat gtagcctata aaacccagta
     1141 tagcaatata tattattgga tagaacagga atggaactgc gtttgtttca gggtgaaccc
     1201 tgaaaaagtg atttatcagt ctcgtaagtg taaatattcc tgaagccgca aggattgctg
     1261 agccttcagg gtaatctctc ttaattatca tgtaagaagc ccagactgcc accatggcac
     1321 ctatcatttc atatacctgg gttggatgaa ggtctggtga agaaaacaat gcgaacaagg
     1381 agatgcttcc ctggtctgct attgatatgt agtgcttgtg tgcaatactt ccaaagggat
     1441 agctgattgc ccacggtaaa tctgttggca ggccaaagca gcatccattg aggagacagc
     1501 ccattcgcat cagaatcaga ccaactccta gacccgatgc aagattgtct ccaagcttcc
     1561 agggggatat tcccacaagt cttgaaatga gccatccaat agctgcagcc ataagaaggc
     1621 ctcccataag tgaaaacccg ctattgttaa tggcaaatat ctttttagga tatttttcgt
     1681 aatatgaagg gtttatcagc acattcaata ccctggctcc aattggcact gatagggtca
     1741 taatagccaa gggcaggaga ctggacctga tggacaagcc agatctcctg atattgacca
     1801 aagtgattat tataactgca atagcagcta tccacatcat aagactatat gattttagct
     1861 ggtaaacctg tcctaaaaat tgaagcgtta gtgtttcctt catggctacc acctgacttt
     1921 tgactattta ttccctgtcc atgtaccttt gatccatgca tcttcagaca tatctatgat
     1981 aaatgatcca ctgagtgata tggctccatt tgattcagac acatagccct ccatatttat
     2041 tcctgttccc tctatgattc ccacagcaga aagtatccca ttattgtagg tggcaggtat
     2101 gtcggtatta tcaccatcct caccgattat tgacaaggta ccacttccat tttcatcaat
     2161 ctgaagcttc attcctacag ccagtggctg tcctatccgc tcttcaagtg cagcttttac
     2221 catgctgtag acatcgatgt ccataagatc acagccttcc acatcactct cgtcgctgga
     2281 ttcgcttggc tctggtggag gtcccaaatc gaattctgta attatcaagg ctgctgatga
     2341 ccaaattcca ttaagctcat tggcttcagc atatattgat acaccaagtt catttaacaa
     2401 gtactctgca tattctttcc ttctttgttc ctcatcatca gtaccaaacc aaatcaatgc
     2461 tatcatccta agctgttcaa cagctatggc ttcttcatta tggacacctc cggattttat
     2521 aaatttccgt ccagtgtccc ttagaacctt atccttcaag ttcatcaaaa tatccattct
     2581 tgtctccagc tcgtatgatt cggtccagtt aaaggctccc ttattcatta agcccgcatc
     2641 cttgtacatc tccaggatca tattcagctc ttctttcttc ttgtcaattt cagcttcctt
     2701 ctggattctg ctcttgaatt ctttctccag gtctttcttt acgcaggctc ttattttatc
     2761 ctcttcttcc cttgtgaggg gctccatacc agcatctttc cttgcctcat tctgggccct
     2821 tactgcttca agctccaact gtcttgccgc tccccccatc tgactccata cgtcctcaaa
     2881 gttttccttt tccacctgat atccccacca tggaacatta ctgcttgcac catttttaaa
     2941 ggcctgatag gcagcctcaa cctcttcact tttccatgat tggatttggc cttcaattgc
     3001 ggcaaccgcg aatttcccaa cctttgttat cataaactgg tctgcgatat gttctcctat
     3061 gatctttgca gcatccctgt atctaccctc agctacgtat cctgcagcct cactggcctt
     3121 ctccacggtc tcaatcccca aatctccagt caaggattta agggcatttg acagggtaga
     3181 ttcagaaaca ttctcaacaa tcttctttcc aagcagtacc tggagattct ggttgaacat
     3241 ggcgggatca ccggcattta cgctctccac catgcttttc cattcgtcat cctcaagtac
     3301 actgttgtat atttgggcta tcattgactt gttgttgatt ttaagattat ctataagtat
     3361 atgctcaatt gcgctgttca aggcatcctc cgtcatggtg ctggtctggt ccttcagcca
     3421 atctgcaact gcggaattgt caacatactg gtcaatcctt tcatccactg taagcttggc
     3481 ctttgaaaac agtgaaaagt gacttgtggt aaatgtcatg gtctgtgcct gggtatccac
     3541 atctgccttt atgtattccc attccgattc attgaaatat ccgaaataca tggcccctgt
     3601 ttcaaagtca tctccgacct catcaggatt gtatttcatg gttattgtca ccggctgcat
     3661 cagccttact gggccgtcac cagctgtaat ctgtatggga gctccaagtc cattcatctc
     3721 cttctcgtca tagccaggag cactgtcagg gtttaccagt gacacctcag ttggagtgtc
     3781 aaaggttcca cctggaatat gtaccattac tccatggacc tccagatctc cgagcaccac
     3841 atcctcagct ccacttgtgg ctgtaagggg gtcaggaata tcctttgtgt attcccagtt
     3901 tgtaggttca ctggccttga cttcaggccc tcccgtagac gattccgttc ctccacagcc
     3961 tcccaacagc atggaagcag caatgacaat ggctaaaatt ccattaatgc ctttgacttt
     4021 aaacattatt ctccacccct cccttgtttt aaacatattc cttatattaa tttttgcaca
     4081 tcaccgtctt aaagtcaaag tttttttctc tgttggcaca aaaaatgaca aagctgcagg
     4141 aagcctgcag cctaggttag attctttatc cattttccag attttattat tggtattcct
     4201 aaaaaagcct ttactacctc ctccattgtc acaaggagga agacgtactc aactggcagc
     4261 tttaagacca atgctcctat gaatgcaagg ggtaccccaa tcagccagac agtgctaagg
     4321 tcaatcttca ttgcaacctt tgtctctcct ccagccctaa ggactccgac tatggctatt
     4381 gtgttataga cccttatgac aaaggtcagg cccatgaaca cgagaagtct tcttgatact
     4441 gcatacaatg aaggatccaa gcctctgaac agtgataggg gaatgtccgg cccaagggcc
     4501 attactattc caagaaccag tcctgatatg gttgctatga ttatgaagct aattgcatat
     4561 tcataggcct tgtcctcttc cccggcacca accttggagc ctaccataac cccgcatgca
     4621 tttgcaagtc ctctcacaat tacaaaaaag acgttctgga tggtgtttgt cagctgtact
     4681 gcagctgttg cctcttcacc tatccttgca taggcagcgg tgtacataac ctgtcccagg
     4741 gcccagaagc cctctgtaag tatgacagga tagatggtgt cgatatatct cttgacgaaa
     4801 tccttcttcc agctcataag ctctgaaaag cttgctgcaa gaatgctctt tgtaccgtat
     4861 attgcgtata caataagaag gatctcaact attctagcga taagtgttcc cagtgcagca
     4921 cccctaattc ccattgccgg cattccaagc ttgccaaata taagaacata gttcaaagct
     4981 gtatttacgg tgaatgatat gattgatacc ttcaagggaa ctcctggtct tcctgtcgtt
     5041 ctaagggcaa tggaaaaggc aaaggatatg gctgtgatta tatatgagag gccgactatc
     5101 ctaaggtatg atgctccaag ctggattacc tcctgttcct caataaagag acccattaca
     5161 aattcaggca gtaccagggc gatgaaggtg aatattgtac cagcgattgt gcttattgtt
     5221 atggccagac ccaatacctt cttgatgctc aggacatcat cccttcccca gaactgggca
     5281 atgaatatgg atgatcctga gttgatcccg aaggttagaa gtatgtacaa aaagaaaatc
     5341 tgatttgcca gtcccacggc tgctatgctt gattggccca gactgcttat catcagagta
     5401 tctatcatat ttagtgaaga agatattaag ttttgcagtg atactggaac tgctattgcc
     5461 agcattccct ttaagaattc cttgtccttg agaaacttca tgatgttgtt ttcctttca
//
LOCUS       DASRQF010000015         6097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_236769, whole genome shotgun sequence.
ACCESSION   DASRQF010000015 DASRQF010000000
VERSION     DASRQF010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236769"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1777
                     /locus_tag="VFD79_00445"
     CDS             <1..1777
                     /locus_tag="VFD79_00445"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZH92543.1"
                     /translation="LHGLEWVVMTALPEGLLMDRLNEILGMTLLLAIAALIVSGIIYN
                     IMTRRMFKPVDDLLAAAENLSSGDLTQRVEVVRKDEIGRISESFNMVAGRMEFLINNL
                     EDAVSERTRELDQAKTILEGSKDHLQLILDSTAEAILGIDLEGRCTSCNRSSLELLGY
                     RSQEELIGENLHLKIHHSHPEGMPYNADNCKIMKSLREGVGTTSDEEVFWKKNGTYFP
                     VEYHSFPQIKDGKVVGAVITFMDITERKERENKIKYLTFHDNLTGLLNRLGFENVMDQ
                     MDVEEKLPISVIFADINGLKLTNDIFGHTAGDRLIRKASEILVHSTRSQDIVARIGGD
                     EFVILLPNTHRDSGGDVVSRIRKGFSNTTMEAIKCSISLGYDTKTSMEDSLEETMANA
                     ENMMYKDKTLNRRAVNKGMIKNIQETLYSRNPREKRHSMNVSELAARMGVELGLRESE
                     ISRLKRAGLLHDIGKIAVDEEVLSKDTTLDEESEIIKQHSVVGYRLLNMFDETVDLAE
                     YIYSHHEHWDGSGYPRGLRGDQIPLLSRILAVAEVFERLSNKGEGPPAQRIQKAVEEI
                     RKGSESQFDPQVVNALIQIVQEDRG"
     gene            1899..4091
                     /gene="pcrA"
                     /locus_tag="VFD79_00450"
     CDS             1899..4091
                     /gene="pcrA"
                     /locus_tag="VFD79_00450"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004825368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HZH92544.1"
                     /translation="MDYLKGLNERQREAVLHVEGPLLIMAGAGSGKTRVVTHKIAYLM
                     DQKGVSPYEILAITFTNKAANEMKERVAKLIAVDVDKMWMGTFHSICVRMLRRDIDKI
                     GYDRSFSIYDRDDQKTLIKECMKELNLDKEMYKENSLLAKIGELKNTQVTPDEYINDN
                     YGDFRERNIGEAYALYQKKLRFYNALDFDDLLIKGVDLLRENPSILKKYQERFKHIFV
                     DEYQDTNKVQYQFVKLLSERHNNICVVGDSDQAIYSWRHADITNILDFEKDFPGARVI
                     LLEQNYRSTEAILRVANQVIKRNTQRKHKNLWTDKNEGNPAIYTRLEYSEEEAFFVAR
                     KIHHLLYKGYKLQDMAILYRTNVQSRPFEEAFMAEELPYKVIGGLKFYDRKEVKDIIA
                     YLKFIQNPNDNIALKRIINTPKRGIGKATIEKMEDHAQEKGESLYSAVLERTNVDGLS
                     ARAINSIKPFADSMNILMAKKELMGIKEFIQETMESTGYIGELEKEGTIEAKTRIENI
                     KDFLSVALNFEKKNPEGTLEDFLASVSLLSDVDKTTDEDNVISMMTVHSAKGLEFPIV
                     FLVGMEEGLFPISRSMDSLEELEEERRLCYVAVTRAEEQLFITSAKARTIYGRTDYTI
                     PSSFLEEMGDTLEIDTSQEKKKEIKKDPLEVKRTMDYNKDNFIPKPVKKKTADVEVSI
                     GSKVSHEKFGKGMIVSIEEMDNDKKIRIAFDGGGLKTLMLSMAPITII"
     gene            4109..>6097
                     /gene="ligA"
                     /locus_tag="VFD79_00455"
     CDS             4109..>6097
                     /gene="ligA"
                     /locus_tag="VFD79_00455"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HZH92545.1"
                     /translation="MADKERLDELIQLIDRYNYSYYTLDSPEISDAEYDSLYDQLVQL
                     EKDLGIQYDYSPTQRVGGEVLPGFKKHIHLGRLWSLDKSQSEDELRAWAERVARTIRD
                     YNSENEEMLPDPEFVLELKFDGLTVNLTYQDGRLVQGATRGNGIQGEGILPQLKTIRS
                     IPLRIPFKGRVEIQGEGVMPLSALESYNETADEPLKNARNAAAGALRNLDPRITEKRR
                     LSAFFYNTGHIEGTSFKTHMEMVEFLKDNRLPVFPYIKVFRSIDDLMEEIHSQQEGRK
                     SLDVLTDGMVVKINDLRTREVLGYTNKFPRWAMAYKFEAEERTTRVIGVQWNVGRTAK
                     VTPTAILEPVDIGGVTIKRATLNNYDDIERKGVKLNGRVLIRRSNDVIPEILGSMPTE
                     EPVSDIEKPTHCPACGSELYQNGVHIFCPNSLSCKPQLVSRLVHFASRDAMNIEGFSE
                     KTAEIFVEKLDIKDLPDIYEVKYSDIIDLEGFKEKKTQNLLDAIERSKDVSLHSFIYA
                     LGVPNVGIKTAMDLAQKYGSLEGLMDAGQEDLVGIPDIGEIVAKSIVEFFHDDGIKRG
                     IEKLLDEGVRPHHHQVEVEEESIFTGRTLVITGTIQGMTRNEIKDKIIQMGGTVTGSV
                     SKKTDFVIVGAEAGSKLKKAQDLNIPIIGEEELKDIFKK"
BASE COUNT         1945 a         1221 c         1620 g         1311 t
ORIGIN      
        1 cttgcatggc ctggaatggg ttgtaatgac agccttgcct gaaggtcttc taatggacag
       61 gttaaatgaa atcttaggca tgacacttct actggcaatt gcagcactga ttgtttcagg
      121 aataatatat aacataatga caaggagaat gttcaagccg gtggatgacc tccttgctgc
      181 agctgaaaat ctgtcttcgg gcgatttgac ccagagggtg gaagtggtca gaaaggacga
      241 aattggaagg atttccgaaa gcttcaatat ggttgcaggc aggatggagt ttctgataaa
      301 caaccttgag gatgcggtca gtgaacgtac aagagagctt gaccaggcaa aaaccatttt
      361 agaggggagc aaggaccacc tgcagctgat actggattca acagctgagg caatacttgg
      421 aattgacctg gagggaaggt gtacctcatg taacaggagc agccttgagc ttcttggata
      481 caggagccag gaagaactga tcggggagaa cctgcaccta aaaatacacc acagccatcc
      541 agaaggcatg ccttacaatg ctgataattg caagatcatg aagtccctaa gggaaggtgt
      601 tgggacaaca tccgatgaag aggttttctg gaaaaagaat gggacatatt ttcctgttga
      661 gtaccattcc ttcccccaga tcaaggatgg gaaggtagtt ggtgccgtaa ttacatttat
      721 ggatattacg gaaaggaaag agagggagaa caagataaaa tacctaacct tccacgacaa
      781 cctcactgga ctcctcaaca ggctgggatt tgaaaatgta atggatcaaa tggatgtgga
      841 ggaaaagctt cccatctcag tcatatttgc agatataaac ggtctcaagt tgaccaatga
      901 catatttggt cacacggcag gtgacaggct gatcagaaag gcatctgaga ttttggtcca
      961 ttcaaccagg tcccaggata ttgtagcaag aataggtggg gacgagtttg tgattcttct
     1021 accaaacacc catagggata gcggtgggga tgtggtttca aggataagaa aaggcttttc
     1081 caacaccacc atggaggcaa tcaagtgcag catctccctt ggctacgaca caaagacatc
     1141 catggaggac tccttggagg agaccatggc aaatgctgag aacatgatgt acaaggacaa
     1201 gacattaaac agaagggctg taaacaaggg catgatcaaa aatattcagg aaacacttta
     1261 ttcaaggaat ccaagggaaa agcggcactc catgaatgtc agcgagttgg cggcaagaat
     1321 gggtgttgag cttggcctta gggaatctga aataagccgg ctaaagaggg caggtttact
     1381 ccatgacata gggaagatag ccgtggatga ggaggtcctt tccaaggaca ccaccttgga
     1441 tgaggagtcg gaaatcataa agcagcactc agttgtgggc tacagactcc tgaacatgtt
     1501 cgatgagaca gtggaccttg ctgaatacat ctacagtcac cacgagcact gggatggatc
     1561 aggctacccc aggggactca ggggagacca gattccactt ctatccagga tactggctgt
     1621 tgctgaggtg tttgagcgtc tatcaaacaa gggggaagga ccacctgccc aaaggataca
     1681 aaaggctgtt gaagagatta ggaaaggctc tgaaagccaa tttgatcccc aggttgtcaa
     1741 tgccttgatt cagattgtgc aggaggatag agggtaggat gtaatgtata aagggaccgg
     1801 tgccaggttt tcattaaaga ccaggaaccg gttctttttg tggaaatctt ccagtcaata
     1861 ctatataatt gtagagagaa tagttaggat ggtgacaaat ggattatttg aaaggactaa
     1921 atgaaaggca gagggaggcg gttcttcacg tagaaggacc cctacttatt atggcaggtg
     1981 ctggatcggg aaagaccagg gtggtcaccc ataagatagc ctaccttatg gaccagaaag
     2041 gtgtatctcc ctatgaaata ctggcaataa cctttacaaa caaggctgcc aacgaaatga
     2101 aggaaagggt ggctaagctc atagccgttg acgtggacaa gatgtggatg gggaccttcc
     2161 attcaatctg tgtaaggatg cttagacgtg acatagataa gataggctat gaccgtagct
     2221 tttccatata cgacagggac gaccagaaga cccttataaa ggaatgcatg aaggagctga
     2281 atcttgacaa ggagatgtac aaggagaaca gccttcttgc aaagatcggt gagctgaaga
     2341 acacccaggt gacccctgat gaatatatca acgacaacta tggggacttc agggaaagaa
     2401 acattggaga ggcatatgcc ctctaccaga aaaagcttag attctataac gccctggatt
     2461 ttgacgacct cttgataaag ggagtggacc ttcttaggga aaatccctcc atattgaaga
     2521 agtaccagga gaggttcaag catatcttcg tggatgagta ccaggataca aacaaggtcc
     2581 agtaccagtt cgtaaagctg ctgtcggaaa ggcacaacaa catatgcgtt gttggggact
     2641 cggaccaggc catttattcc tggaggcacg cagacattac aaatattctg gattttgaga
     2701 aggactttcc aggagcaagg gtcatactcc ttgagcaaaa ctaccgttca acagaggcca
     2761 tactaagggt ggccaaccag gtaatcaaaa ggaacacaca gagaaagcac aagaaccttt
     2821 ggacagacaa gaatgaaggg aacccagcca tatacaccag gctggaatac tcagaggagg
     2881 aggccttctt tgtagcgaga aaaattcacc accttctcta taagggctac aagctccagg
     2941 atatggcaat cctctacagg acaaacgttc agtccagacc cttcgaggag gcatttatgg
     3001 ctgaggagct tccctacaag gtgattgggg gactcaagtt ctacgaccga aaggaagtaa
     3061 aggacataat cgcatacctg aaattcatcc aaaacccaaa tgacaatatc gccctgaaaa
     3121 ggatcataaa taccccaaag agggggattg gaaaggcaac aatagaaaaa atggaggacc
     3181 atgcccagga gaagggagaa tccctataca gtgcagtcct tgaaaggacc aatgtggatg
     3241 gactatctgc aagggccata aattcaatca aaccttttgc agattcaatg aacatcctca
     3301 tggcaaagaa ggaattgatg gggatcaagg agtttatcca ggagaccatg gaatccacag
     3361 gatatatagg cgagcttgaa aaggaaggga caattgaggc aaagacaaga attgagaata
     3421 taaaggactt cctttctgta gccctgaatt ttgaaaagaa gaatccggaa gggacactgg
     3481 aggactttct tgccagcgta tccctgcttt cagatgtgga caagaccacc gatgaggaca
     3541 atgtcatatc aatgatgacc gtccactctg ccaagggact tgagtttcca attgtcttcc
     3601 ttgttggaat ggaggaggga cttttcccca tctcaaggag catggactcc ctggaagagc
     3661 tggaggagga aagaaggctt tgctatgttg cggtgacacg ggctgaggaa cagcttttca
     3721 tcaccagtgc aaaggccagg accatttatg gaagaacaga ctacacgata ccctcaagct
     3781 tcctggaaga aatgggagac actttggaaa tagacacctc acaggaaaag aaaaaggaga
     3841 tcaaaaaaga cccccttgag gtcaaaagga ccatggatta caacaaggat aatttcattc
     3901 caaaaccagt gaaaaagaag actgcagatg ttgaggtatc aatcggaagc aaggttagcc
     3961 acgaaaagtt tggaaaagga atgattgtaa gcattgaaga gatggataac gacaagaaga
     4021 tcaggatagc ctttgacggt ggagggctca agacccttat gctgtccatg gcacccatta
     4081 caataatatg attaaaggag agattggcat ggctgacaag gagagattgg atgaactcat
     4141 tcagcttata gaccggtaca attacagcta ctacacccta gacagtccgg aaatcagtga
     4201 tgcagagtac gacagcctgt acgaccagct tgttcagctg gaaaaggacc ttggaataca
     4261 gtacgactac tcacctaccc agcgtgtggg tggagaggtc ctgccaggct tcaagaagca
     4321 catccatcta gggagactgt ggagtcttga caagagccaa agtgaggatg agcttagggc
     4381 ctgggccgaa agggtggcca ggacaatccg cgactacaac agtgaaaatg aagaaatgct
     4441 tccagaccct gaattcgttc tggagcttaa atttgacgga cttacggtaa atctgaccta
     4501 ccaggatgga aggctggtcc agggtgccac aaggggcaat ggaatccagg gagagggaat
     4561 ccttccacaa cttaagacca taaggtcaat tccattgagg attcccttca agggaagggt
     4621 tgaaatacaa ggggaggggg taatgcccct ttcagctctt gaaagctaca atgaaaccgc
     4681 agatgagccc ctgaaaaatg caaggaatgc agcagctggg gccttgagaa atcttgaccc
     4741 tagaattact gaaaaaagaa ggctatctgc atttttctac aataccggcc acattgaggg
     4801 gacaagcttc aagacccaca tggaaatggt tgaattcctt aaggacaaca ggctgccggt
     4861 gttcccatac atcaaggtat tcaggtcaat cgatgatctg atggaggaga tccacagcca
     4921 gcaggaagga agaaaaagcc tggatgtcct gacggacgga atggttgtaa agataaatga
     4981 cctaaggacc agggaggttc ttggctatac aaacaagttc ccaagatggg caatggccta
     5041 caagtttgag gctgaggagc ggaccacaag ggttattggg gtccagtgga atgttggaag
     5101 gacagccaag gtgacgccaa cggcaattct tgagcctgtt gatatcggtg gggtgaccat
     5161 aaaaagggca accctcaaca actatgatga tatagagaga aagggagtaa agctaaacgg
     5221 gcgggttctc atcaggcggt caaatgatgt tattccagag atccttggca gcatgccgac
     5281 agaagaacct gtaagtgaca tagaaaagcc aacccattgt ccggcctgcg gcagcgagct
     5341 ttaccagaac ggggtccaca ttttctgccc caactcactc tcctgcaagc cccagctggt
     5401 gtcaaggctt gtacactttg ccagcaggga tgccatgaat atcgagggct tttcagagaa
     5461 gacagcggag atatttgttg aaaagctgga tataaaggac ctgccggata tttacgaggt
     5521 taaatacagt gatataatag acttggaggg cttcaaggag aagaagacac agaatcttct
     5581 tgatgccatt gaaaggagca aggatgtaag cctccattcc tttatctatg cccttggagt
     5641 tcccaacgtt ggcataaaga cggcgatgga cctggcccag aagtatgggt ccctggaggg
     5701 actcatggat gccggccagg aagaccttgt tggaattcca gacattggag agatcgttgc
     5761 caaatcaatt gtggagttct tccatgatga tgggatcaaa agagggatag aaaagcttct
     5821 tgatgaaggg gtaaggcctc accaccacca ggtggaggtg gaggaggaat ccatattcac
     5881 tggaaggact cttgtcataa caggaaccat ccagggtatg acaaggaatg aaatcaagga
     5941 taaaatcatc cagatgggag gaactgtaac aggttctgta agcaaaaaga cagattttgt
     6001 cattgtggga gcggaggcag gaagcaagct taaaaaggca caagacctta acatcccaat
     6061 tatcggggaa gaggagctta aggatatatt taaaaag
//
LOCUS       DASRQF010000016         4798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_859954, whole genome shotgun sequence.
ACCESSION   DASRQF010000016 DASRQF010000000
VERSION     DASRQF010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859954"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..2263)
                     /locus_tag="VFD79_00460"
     CDS             complement(80..2263)
                     /locus_tag="VFD79_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92546.1"
                     /translation="MFRRVIAIILVFSMMVSISGCGGVAAGKEKVLAEGVITEENSSL
                     SGEGLGLHIDPVNFTGELKAKITSVPGSHPLGEDGEIQLQVYDFSVEGMTEFPGVIEL
                     TIPLQVGQGEEPGAAYLNPETGAWEPVVFRYDDSSKTLTILTDHLSRYGVFSYSDQGK
                     RYATAQYLGLYGVGEDHDFLQAVEEFSVAGVPAAQCIEIGASAAGDALGIGGDMIGNI
                     VQGAGYMAYGEDILSSIGDSLGKIGLLVSVVQIGSNIYNNKINDALAGSMKTAFTYIM
                     GKVAGKLSSSVMSASMASVAIVDYSINKFGTTAIEGRKAIYRDAYSIYYQRGEDGFRT
                     SAHWFDLFYPYFSNPTMTQDEIKAQVDKIVTDHCAEFWGGTNELGIDYYVSEARKRFN
                     WTGGQAGLNDDMRKEISAERRAVLYNDILPGVFEHIAFKINLENEKRLNAEYANLVKF
                     LNTQVKFSIVDQEGIYDKHRVRFSPLNDKAIVENWTGTLKKGTVMSSMFTLYGHMYAG
                     GPDTVDIYEPDADLEKDKPIRSVQFKVVIPDFEIVLGDVIKGLTFEGGERDKVSQMGL
                     YAALREAGTIKVAEDGTFSFDVPYATANGGGGNNTFSSEVSGFHMEGKLDPGSLSGNA
                     TFNGTFLFRRREISPLEGMDGETKEYVTTYAYDDIVNGTVDISGEGETVTFNTRLEAV
                     RSGFSKLQYHSISPDGTEFWGDNPTITDKSENYNGSSTYTFKVAR"
     gene            2706..>4798
                     /locus_tag="VFD79_00465"
     CDS             2706..>4798
                     /locus_tag="VFD79_00465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZH92547.1"
                     /translation="MKQIHKRIRMIILIIVAVVFLSIFIGINEPISSSLEDGVLNEFQ
                     LTAHNKYQVLNERLINISNNAQSLSSRTSIKNAMRDYLEGETTFQDLRDYLVPRYEEG
                     VSVFERSRTAGRVLANGQTLHVVGPDTNIKNYIDWDETEVKMSHYSQGVVVAQSPIMD
                     GDEILGFDVVLTDISPIIRNMEEGGYKVEFLSSEHEDTVLRENGLVTSFIYSDHIHKT
                     VAVSMDENHVLGPLIQFRKSLYFRYFMVLLVVFLIVQFLGVKYIQKFIREQERLKNAA
                     EESEKDKRALIDEMNQGFLLVRESKGDHDDFRDYEIMEVNSTFEGMASTSRDELIGKS
                     LYQLVTIVSKVDMDQFDKVLDSGESKQFEYYVKDLDKWWNLSVYRPRESYLAIVLDDI
                     TEKKKIELRLKDQERTLRYTLNIAGEGLWDWNFEEGIVRHNKKWCEIMGVPYDTDYHR
                     LGGFVAQVHPEDEDKLRDSIARAVSGEENLYSEHRMVLQDGRTIWIRDRGALLRDQDG
                     KPVRMIGSMADITAQKRAEENLFLEKEIFQSTLLSVEDGIISTDVDGRISILNPAAEK
                     MTGWTFQEAKGRQLVDVFKLYHPETGEPVDILEVARKRGNGHHKQALLVTKTEEELVV
                     NNSVATVRLQDGQTMGYVIVFRDITQSVNKQKRIEYLSFHDELTGLYNRRYVEDAVKR
                     MDTPRNLPFTVMIVD"
BASE COUNT         1351 a         1053 c         1125 g         1269 t
ORIGIN      
        1 ggaaagacag aatcggagtg tgtgttcttt tgtaccttaa tagtaacaga ttgtgggcat
       61 aaattcaata tccttttcac tatcttgcaa ccttgaaggt atatgtgctt gacccattgt
      121 agttctcgga cttgtctgta atggttggat tgtctcccca aaattcagtg ccgtcgggac
      181 tgatcgagtg gtactgaagt tttgaaaacc ctgaacggac agcttcgagc ctggtgttga
      241 aggtgacagt ttccccttca ccggaaatat ccactgtacc attgacaata tcatcatagg
      301 cataggttgt gacatattcc ttggtttccc catccatacc ctccagtgga ctgatttccc
      361 ttcttctgaa aaggaaggtc ccgttgaagg tggcatttcc tgacagggat ccggggtcca
      421 gctttccttc catgtggaag ccggaaacct ctgaggagaa ggtattgttg ccgcctccac
      481 cgtttgctgt ggcatatggg acatcaaatg agaaagtccc gtcctctgca acctttattg
      541 tacctgcctc cctgagggct gcatagagac ccatctgtga aaccttgtcc ctttcacccc
      601 cttcaaaggt aagtcccttt ataacatctc caagaactat ctcaaaatca ggaataacaa
      661 ccttgaactg tacactcctt attggcttgt ccttttcaag gtctgcgtcc ggctcatata
      721 tgtccacagt gtcaggtccg ccggcataca tatgaccata gagggtgaac atggaggaca
      781 tgaccgttcc cttctttagt gttcctgtcc agttctcaac aatagccttg tcattaaggg
      841 gtgagaacct taccctgtgc ttgtcgtaga ttccctcctg gtccacaata ctgaatttga
      901 cctgagtatt gaggaacttc acaagatttg catactccgc gttaagtctc ttctcatttt
      961 caagattgat cttgaaggcg atatgttcaa acactccagg aaggatgtcg ttatatagga
     1021 ctgcccttct ttcagctgaa atctccttcc tcatatcatc attaaggcct gcctgtccac
     1081 ctgtccagtt gaatcttttc ctggcctcac tgacatagta gtcaattcca agctcgttag
     1141 tgccacccca gaattcagcg caatggtcag tgactatttt atccacttga gccttgatct
     1201 catcctgggt catggttggg ttgctgaaat agggatagaa gaggtcaaac cagtgggctg
     1261 aggttctgaa tccatcctcg cccctctgat agtaaatgga gtaggcatcc ctgtagatag
     1321 cctttctgcc ttcaattgcc gttgtaccaa acttgtttat ggaatagtcc acaatggcaa
     1381 cagatgccat gctggctgac atgacagagc tgctgagctt gcctgccacc ttgcccatta
     1441 tatatgtaaa ggctgtcttc atggagcctg caagagcatc attgatcttg ttgttgtaaa
     1501 tatttgaacc gatttgaaca accgacacca atagaccgat cttgcccagg gagtctccaa
     1561 tgctgctgag gatatcctcc ccatatgcca tgtagcctgc accctgaacg atgtttccta
     1621 tcatgtcacc gccaattcca agggcgtctc ccgctgcgct tgcaccaatc tcgatgcatt
     1681 gtgcagctgg gacacctgcc actgaaaact cttcaaccgc ctgcaggaag tcatgatcct
     1741 ccccaacccc atagaggccc aggtactggg ctgttgcata tcttttcccc tggtctgagt
     1801 aggagaagac tccataccgg gaaaggtggt ccgtcagtat tgttaaggtc ttgcttgaat
     1861 cgtcatatct gaaaacaaca ggttcccagg ctcctgtttc agggttcagg taggctgcac
     1921 caggctcttc accctgacca acctggagag gaatggtcag ctcaatcact cccggaaatt
     1981 cagtcatacc ctcaactgag aaatcatata cctggagctg gatttcacca tcctcaccca
     2041 agggatgtga accaggtacg gaggtgatct tggccttaag ctctcctgta aagttcacag
     2101 gatcgatatg taaacccagt ccttccccgg agagggagga gttctcttca gttatcaccc
     2161 cttcagcaag gaccttctcc tttccagctg ccacaccacc gcagcctgaa atggacacca
     2221 tcatggaaaa aaccaaaatt attgcaataa cccttctgaa catatctagc cctccttttg
     2281 cacattatgg tataaaagta atattggtta attccagtat actcctattt taatgtaaaa
     2341 tcaccaataa ttatttgggt tgtcacagtt tgtgacagaa ttggacattg taaaataagt
     2401 ctgtataaag tgtattttaa tgaaaatatt aggcttaata atgcatccat caagaaattg
     2461 ctgtagaaga gtccaatagg ggactcggtg aatcttgaaa ttgagacatt ctaaaaatca
     2521 agtgctctga tgaaatcatt caccaacaat tgacagaaaa ttatcaataa tgactacttg
     2581 ataattatat gataaaatag tatggtctga gagtttgcat ttttactgga aatcagataa
     2641 ttatatacaa cttgcaatcg ttgcattatc aattagaact gcaataacca gggggagggg
     2701 aagacttgaa acagattcac aagagaatta gaatgataat cctaataatt gttgctgttg
     2761 tattcctatc catcttcatt ggtataaacg aacccatatc ctcaagcctt gaggatgggg
     2821 tgctcaatga gtttcaattg actgcccaca acaaatacca ggtcttaaac gaaagactga
     2881 tcaatataag caacaatgcc cagtccctat ccagcaggac aagcattaaa aatgccatga
     2941 gggactacct tgagggagaa accaccttcc aggaccttag ggactacctt gtacccagat
     3001 atgaagaggg agtttcagtt tttgaaaggt ccaggacagc tggtcgggtc cttgccaatg
     3061 gacagaccct tcatgttgta ggccccgata ccaacattaa gaactacatt gattgggatg
     3121 agacggaagt taaaatgagc cactactcgc aaggtgttgt tgtggcccag tccccaataa
     3181 tggatgggga tgaaatcctg ggattcgatg ttgtactcac ggatataagt cccattatca
     3241 gaaatatgga agagggaggc tacaaggtgg aatttctctc ttctgaacat gaagacaccg
     3301 tattaagaga gaacggtctg gtgacttcat ttatttacag tgaccatatt cataagaccg
     3361 ttgcggttag catggatgag aaccatgtcc tcgggccctt gatccagttt aggaagagtc
     3421 tctattttag gtatttcatg gttctcctgg tcgtttttct tatagtccag tttttgggag
     3481 tcaagtatat ccaaaaattc atcagggaac aggaaaggct taagaacgct gcagaggaaa
     3541 gcgagaagga taagcgggcc cttatagatg aaatgaacca gggatttctc ttggtcaggg
     3601 aaagcaaagg ggatcatgat gattttaggg actacgagat tatggaagtg aactctacct
     3661 tcgaagggat ggcgtcaact tcaagggatg agctaattgg aaaatcattg taccagctgg
     3721 tgacgattgt cagcaaggtg gacatggacc agtttgacaa ggtcctggat agtggagagt
     3781 ccaagcaatt tgagtattac gtgaaggacc ttgataagtg gtggaacctg tcagtgtaca
     3841 gaccaaggga gtcatacctg gctatagttc tggatgacat aactgaaaag aagaagattg
     3901 agctgaggct caaggaccag gaaaggaccc ttcgatacac cctgaatatt gctggtgaag
     3961 gcctttggga ctggaacttt gaagagggaa ttgtccggca caacaagaag tggtgtgaga
     4021 tcatgggagt tccatatgac acggactacc acagactagg tgggtttgtt gcccaggtcc
     4081 atccagagga tgaagataag ctaagggata gcatagcaag ggcagtgagc ggagaggaaa
     4141 acctatacag tgaacacagg atggtccttc aggatggtag gacaatctgg atcagggaca
     4201 ggggcgcatt gctgagggac caggatggca agcctgtaag aatgattggt agcatggcgg
     4261 atataacagc acagaaaagg gctgaggaga atctgtttct tgagaaggaa atcttccagt
     4321 caaccctctt gtcagttgag gatggtatta tttccacaga tgttgatggc aggataagta
     4381 ttctaaatcc agccgctgag aagatgaccg gttggacctt ccaggaggct aaaggcaggc
     4441 agcttgtgga tgtatttaaa ctctaccacc cagaaaccgg agagcctgta gatatccttg
     4501 aggtggctcg caagagggga aatggacacc acaagcaggc ccttcttgtg accaagactg
     4561 aagaggagct tgtggtgaac aacagtgttg ccacagttag acttcaggac ggtcaaacca
     4621 tgggatatgt aatagtattc agggacataa cccaatctgt aaacaagcag aagaggatag
     4681 agtacctgag cttccacgat gagcttactg gcctctacaa cagaaggtat gtggaggatg
     4741 cagtaaaaag aatggacacc ccaagaaacc ttccatttac ggttatgata gttgacat
//
LOCUS       DASRQF010000017         2046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_730790, whole genome shotgun sequence.
ACCESSION   DASRQF010000017 DASRQF010000000
VERSION     DASRQF010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730790"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VFD79_00470"
     CDS             complement(<1..329)
                     /locus_tag="VFD79_00470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZH92548.1"
                     /translation="MTYDSKGKISIIIPILNEAGTVGEIMDNLEKLRGDFEIIFVDGG
                     SDDGTDTMVEKRYSMVRSPRKGRSFQMNYGALLAQGSILLFLHADCRLSEDALDKIRC
                     EISKGSKV"
     gene            complement(319..1308)
                     /locus_tag="VFD79_00475"
     CDS             complement(319..1308)
                     /locus_tag="VFD79_00475"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HZH92549.1"
                     /translation="MDLGKIRKDKDFIEIFGLIDPDGFQPLGQGEYNINYIFNSKVTG
                     EKLVLRIATDSQMHLENQIGYEFKALELLANSGRTPRPFYCDSRKTLIPYGFLVMEFL
                     PGVPLNYKRDLKLAAQALSEVHSLVIPKDNHLLLPEDPIGAIYEECKMMFDEYSSSAH
                     MEVSVRYKIDSLLDMVEGMEAEDIGNRVMINTEINSGNFLINGTDKPNYLIDWEKPLY
                     GYPAQDLGHFLAPTTTSWKTDTILSKDEMLLFISDYCRMSKGGYVVSELWNSVRRYVS
                     MNCLRGITWCSMAYSLYQDPDKLITNDYTYRKIKTYLSEDFLDMIRKEYLGDI"
     gene            complement(1310..>2046)
                     /locus_tag="VFD79_00480"
     CDS             complement(1310..>2046)
                     /locus_tag="VFD79_00480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HZH92550.1"
                     /translation="RFTTQNKMKGQREESMKGKGMKDKALILFTRIPKLGRIKTRLEP
                     FLGKEQCVKLQTAFIKDIYDKLKDKDMDIIISYTEEGDLDVLRGIAGSDARLIKQVGP
                     DIGDKMRNAIKKVLSEYKRVVLIGSDIPLLEKSDIETAFQVLETRDMVISPTYDGGYY
                     LIGMKKDHPEVFGIQYSTSSVFEETVERIRMLGKSYQRGSLQLDIDDKEDFLKLHRIL
                     KDDRDIECRETRRVVNQIMEMKAESE"
BASE COUNT          556 a          516 c          316 g          658 t
ORIGIN      
        1 accttggacc cttttgatat ttcgcatcta attttatcca aagcatcttc agaaagtcta
       61 caatcagcat gcagaaacag aagtatgctt ccctgggcca atagagctcc gtagttcatc
      121 tgaaaggacc tccccttcct gggagaacga accatactgt accttttctc aaccatggta
      181 tctgtaccat catcactccc cccgtcaaca aatattattt caaagtcccc tcttagcttt
      241 tctagattat ccataatttc gcctacggtc ccagcttcgt ttaatattgg aataattatg
      301 gaaatcttac ccttagaatc atatgtcacc taaatactcc tttcttatca tatccaaaaa
      361 atcctctgaa aggtaggtct taatctttcg gtatgtatag tcgtttgtaa tcaacttatc
      421 aggatcttgg tagagtgagt atgccatgga gcaccaggtt atccctctaa ggcaattcat
      481 agagacgtat cttctcacgg agttccacag ttcagataca acatatccac cctttgacat
      541 cctgcagtag tcactaataa atagcagcat ctcatccttg gacagtattg tatcagtttt
      601 ccacgaagtt gttgtagggg ccaaaaaatg ccctaaatcc tgagcagggt acccatacaa
      661 aggcttttcc caatcaatta aataattggg tttgtcagta ccattgatca aaaaattacc
      721 tgagttaatt tctgtgttga tcattaccct gttgcctata tcctcagctt ccattccctc
      781 gaccatatcc aagagtgaat cgatcttata cctaacagag acttccatat gggctgaaga
      841 agagtattcg tcaaacatca tcttacattc ctcatatatt gccccaatgg gatcctcagg
      901 caacagaaga tgattatcct taggtattac caggctatga acttctgaca aggcttgagc
      961 tgccaatttc aaatcccttt tatagttcag cggtactcca ggcaagaatt ccataaccaa
     1021 aaacccataa ggaataagtg ttttcctact gtcgcagtag aaaggcctag gtgttctccc
     1081 tgaatttgcc aaaagttcaa gggctttgaa ttcatagcct atctgattct caagatgcat
     1141 ctgactgtca gttgcaatcc ttaatacaag cttttcgcct gttactttgg aattgaaaat
     1201 ataattaatg ttatactctc cctgtccaag tggctgaaat ccatccggat caatcagtcc
     1261 gaagatttcg ataaaatcct tgtcttttct tatcttaccc aaatccatcc tattcactct
     1321 ccgccttcat ctccattatc tgattaacta ctcttctggt ttccctacac tcaatatccc
     1381 tgtcatcttt caatatcctg tggagtttaa gaaagtcctc cttatcatcg atatccaatt
     1441 gcaggcttcc ccgttgatag gatttcccaa gcatcctaat tctttccact gtctcctcaa
     1501 atacagaact ggtgctgtac tggattccaa atacctcagg gtggtccttc ttcatgccta
     1561 tgaggtagta gcctccatca taggttggag aaatcaccat atctcttgtt tccaacactt
     1621 gaaacgcagt ttcaatatca gatttttcta gcaagggtat gtcgcttcca ataagaacaa
     1681 cccttttgta ctcactcaaa accttcttta ttgcatttct catcttgtct ccaatatcag
     1741 gccccacctg cttaatcaac cttgcatcac tgccggcaat cccccttaaa acatccaggt
     1801 caccctcttc agtgtagctg attattatgt ccatgtcctt gtccttgagc ttgtcataga
     1861 tatcctttat aaaggcagtc tgaagcttta cacactgctc cttcccaaga aaaggctcca
     1921 gtcttgtctt aattctacct aatttgggaa tccgtgtaaa aagtattaaa gccttatcct
     1981 tcataccctt acctttcatt gactcttccc gctgtccttt catcttgttt tgagttgtaa
     2041 accttt
//
LOCUS       DASRQF010000018         3460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1606652, whole genome shotgun sequence.
ACCESSION   DASRQF010000018 DASRQF010000000
VERSION     DASRQF010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1606652"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /locus_tag="VFD79_00485"
     CDS             <1..1479
                     /locus_tag="VFD79_00485"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HZH92551.1"
                     /translation="SGRWENFGPEMWRLKDRNDREFCLGPTHEEYFTDLIKDEIKSYK
                     QLPLSIYQIQTKYRDEKRPRFGLMRCREFIMKDAYSFDTDEEAMKVAYQNMWDAYDRV
                     FTRLKLNYKVVQGDTGAMGGKVSHEFTAMSDVGEGLVAYCNSCDFAATDEKAQVIHSI
                     PQGEEEEKEMDKVLTPEVKTIDGLVDFFNIERYRFGKALVFLAQGEPVVVVIPGDREL
                     NETKLANYLMIPGHELEMADEETIKKISGANKGFTGPVGLAEGTRLLVDQRVTGLKNI
                     VVGGNETDYHLVNVNYGRDFTGEVVEDLLMVEAGDTCPTCGEKLFLDRGIEVGNIFQL
                     GTKYSESLKATYLDVNGKEQPFVMGSYGIGVSRSVAAVVEQFHDDKGIVWPFVVAPYH
                     AVVTIVNTKKDEQNELGERIYEDLKKMGVEVLLDDRNERPGVKFTDRELIGIPVQITV
                     GKMASEGIVEYSLRKREGKQEVTADKIRDLILEAFEEEGFSL"
     gene            1540..3021
                     /gene="gltX"
                     /locus_tag="VFD79_00490"
     CDS             1540..3021
                     /gene="gltX"
                     /locus_tag="VFD79_00490"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HZH92552.1"
                     /translation="MSDIRVRFAPSPTGYLHIGGLRTALYNYLFAKKNGGKFVLRIED
                     TDQTRFVEGAIENLIDSLHWAGVQYDEGVFVEDGKIVEKGDFGPYVQSKRLDIYRKYV
                     DELIENGHAYYCFCSKERLETVREEQKMKGMVPKYDGFCRNVSLEDAKKRVAAGEDHV
                     VRLKLPHNRDIHFHDLVRGDITINTNDIDDQVLLKSDGFPTYHMAVVVDDHLMGITHI
                     VRGEEWLPSTPKHVYMYEAFGWEKPTYVHLPTVLNKERKKLSKRHGDVSVDDFRTKGY
                     LPEGLVNYLALVGWSPDGNEEILSMEEMVDKFSFDRVSKTGGIFDKDKLDWVNSHYIR
                     SESLQRIKDMTLPYLVEAGFMDEDFARDNSQWVDVLIETVREGLATVNEVVDKVEFIF
                     EENLVYEAEAYDEFLNNENGRALMEAIKKEAMDSDQVTMENAKDFMKRIQKNTGIKGK
                     NLFMPTRVALTGSAHGPELVNVLYLLGNEKIAQRAQKVLDIID"
     gene            3156..>3460
                     /locus_tag="VFD79_00495"
     CDS             3156..>3460
                     /locus_tag="VFD79_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019108494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZH92553.1"
                     /translation="MKLFNSLTRKKEEFVPLVPGQVSMYNCGPTVYNFIHVGNARPLV
                     TFDTLRRYFIYKGYDVRFVLNFTDIDDKLINKAKEENITVKEVADRYIQAFKEDAGAL
                     "
BASE COUNT         1036 a          651 c          961 g          812 t
ORIGIN      
        1 tcagggagat gggaaaattt cggtcctgag atgtggagat taaaggacag gaacgaccgt
       61 gaattctgcc ttggaccaac acatgaggag tatttcacag acctgatcaa ggatgaaatc
      121 aagtcctaca agcagcttcc acttagcatc tatcagatcc agaccaagta cagggatgaa
      181 aagagaccaa gatttggcct tatgagatgc agggagttca taatgaaaga tgcctacagc
      241 ttcgatactg acgaggaagc aatgaaggtg gcctaccaga acatgtggga tgcctatgac
      301 agggtgttca caagactcaa gctgaactac aaggttgttc agggtgacac gggagcaatg
      361 ggaggaaagg tttcccatga gttcactgcc atgagcgatg ttggcgaagg ccttgttgcc
      421 tactgcaata gctgtgactt tgcagccacc gatgagaagg cccaggtcat acattccatc
      481 cctcaaggag aagaggagga gaaggaaatg gacaaggtcc tgactccaga ggttaagacc
      541 atagacggcc ttgtggactt cttcaacatt gaaaggtaca ggttcggtaa ggcccttgta
      601 ttccttgccc agggcgagcc tgttgttgtg gtgattccag gagaccgtga gctcaatgaa
      661 accaagcttg caaactatct gatgattcca ggacatgagc tggaaatggc cgatgaagag
      721 acaatcaaga agatttcagg tgccaacaag ggctttacag gacctgttgg acttgccgag
      781 ggaacaaggc tactagttga ccagcgcgtt acaggcctta agaatattgt agtgggtgga
      841 aacgagacgg attaccacct ggtaaatgtc aattacggaa gggacttcac aggagaggtt
      901 gttgaggacc ttcttatggt tgaagcggga gatacctgcc cgacctgtgg agaaaagcta
      961 ttccttgaca ggggaattga agtggggaac atcttccagc ttggtaccaa atacagtgag
     1021 agcctcaagg caacctatct tgacgttaac ggcaaggaac agccctttgt catgggaagc
     1081 tacggaatag gtgtttcaag gagtgtggct gctgtggttg agcagttcca cgatgacaag
     1141 ggtatagtgt ggccttttgt agttgcgccc taccatgctg ttgtaacaat tgtgaacacc
     1201 aagaaggatg agcagaatga actgggagaa aggatctatg aagaccttaa gaagatgggt
     1261 gttgaggtcc tgctagatga tagaaacgaa agacccggtg tgaagttcac tgacagggag
     1321 cttattggga ttccagtcca gattaccgtc ggaaagatgg cttcagaggg gattgttgag
     1381 tattccctta gaaagaggga aggcaagcag gaggtcactg ctgacaagat cagggatttg
     1441 attcttgagg catttgagga ggaaggattt tctctttagt tgccacgatt aagggtatta
     1501 aattacagag gattgatata aattcaggag gtgtgtgaat tgagtgatat aagagttaga
     1561 tttgccccca gtccaacggg atatctccat attggaggat tgagaaccgc tttgtacaac
     1621 tacctattcg caaagaagaa tggcggtaag tttgtactta gaattgagga tacagaccag
     1681 accaggtttg ttgaaggagc aattgagaac ctgattgatt cccttcattg ggccggtgtc
     1741 cagtatgacg agggagtatt tgtggaggat ggcaagatag ttgaaaaggg agacttcggt
     1801 ccctacgttc agtcaaagag gctggatatc tacaggaaat atgttgatga actgatagag
     1861 aatgggcatg cctactattg cttctgctcc aaggaaagat tggagacagt cagggaagag
     1921 cagaagatga agggaatggt tccaaaatat gacggcttct gcagaaacgt ttccctggag
     1981 gacgccaaga agagggtagc tgcaggtgag gaccatgtag tcaggttgaa gcttccccac
     2041 aacagggaca ttcattttca tgaccttgta cgaggggaca taacaataaa cactaacgac
     2101 atagacgacc aggtgctatt gaagtctgac ggcttcccaa cataccacat ggcagttgtt
     2161 gtggatgacc accttatggg aatcacccac atagtaaggg gagaggagtg gctcccatca
     2221 acaccaaagc acgtatacat gtacgaggcc tttggctggg agaagccaac ctatgtgcac
     2281 ctgccaacgg ttcttaacaa ggagaggaag aagctgtcca aaagacatgg tgatgtttcc
     2341 gttgacgact tcagaaccaa gggctacctg ccagaggggc ttgtgaacta ccttgccctg
     2401 gttgggtgga gtccagatgg caatgaggaa atactttcca tggaggagat ggtggacaag
     2461 ttctcctttg acagggtctc aaaaaccggt ggaatatttg acaaggacaa gcttgactgg
     2521 gtaaacagtc actatataag aagtgaaagc cttcaaagga taaaggacat gaccctgccc
     2581 taccttgttg aagccggctt tatggatgag gattttgcaa gggacaactc ccaatgggtt
     2641 gatgtcctga ttgaaaccgt aagggaaggg cttgcaacag taaatgaagt tgtagacaag
     2701 gttgaattca tatttgaaga gaaccttgtg tacgaagctg aagcctacga tgagtttttg
     2761 aacaacgaaa atggacgggc ccttatggag gcaatcaaga aggaagcgat ggattcagac
     2821 caggttacca tggaaaatgc aaaggacttt atgaaaagga tccagaagaa taccgggatc
     2881 aagggcaaga atctctttat gcctacaagg gttgcattga ccggcagtgc acacggacct
     2941 gagcttgtga atgtactcta tcttcttgga aatgaaaaga tagcccagcg ggctcaaaag
     3001 gtgctggata taattgactg atggctgctt catataatag aatgagagta gcaaagactg
     3061 gttttgaccc ttgtggaaaa ccatgattca ggttgaaaat cgtctctgct cgtttcaggg
     3121 acgatttttg ttgagtaatg ttaaggaggg cacgtatgaa gctatttaat tcattgacaa
     3181 gaaagaaaga ggaatttgta ccattggtcc cggggcaggt cagcatgtac aactgtggac
     3241 caactgtgta caactttatt cacgttggaa acgcaaggcc tcttgtgacc tttgatacct
     3301 tgagaagata ctttatatac aagggctatg acgtaaggtt tgtactgaac ttcacagaca
     3361 tagacgacaa gcttataaac aaggccaagg aggagaacat aacagtcaag gaggttgccg
     3421 acaggtacat ccaagccttt aaggaggacg ctggagccct
//
LOCUS       DASRQF010000019         4964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1476396, whole genome shotgun sequence.
ACCESSION   DASRQF010000019 DASRQF010000000
VERSION     DASRQF010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476396"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..836)
                     /gene="sfsA"
                     /locus_tag="VFD79_00500"
     CDS             complement(138..836)
                     /gene="sfsA"
                     /locus_tag="VFD79_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HZH92554.1"
                     /translation="MRYSNMVEGTFLKRPNRFIAHVLINGREEIAHVKNTGRCRELLL
                     PGARVILQDHGLSTKRKTRYSLISVYKEDMLVNMDSQVPNRVVMDALMEGKIQGFGTP
                     DLIKGEQKFGDSRFDIYYRDGDREGFIEVKGVTLEMDGLSKFPDAPTVRGTKHIMEMI
                     KAVQEGYEGNIFFLIQMKGPMAFTPNRLMDPEFGNALDLAIKEGVGLHVYDSLVTQDS
                     IQIGSKIQYREQAE"
     gene            939..1442
                     /locus_tag="VFD79_00505"
     CDS             939..1442
                     /locus_tag="VFD79_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005397894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HZH92555.1"
                     /translation="MRQLTKITILTLVMALLLIGFAGCSGLDVVVEEPEAPGVENPAS
                     EEPQELVEEAIAEDGYYTSPEEVALYLHTYARLPDNYITKEDAMDLGWDAQKGNLWDV
                     TDRMSIGGNRFGNRERLLPEAEGRQYYEADVNYEGGYRGAERLVYSNDGLIFYTGDHY
                     ESFEQLY"
     gene            1454..1732
                     /locus_tag="VFD79_00510"
     CDS             1454..1732
                     /locus_tag="VFD79_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HZH92556.1"
                     /translation="MKIILIDAKKMRDRDFHDYFRRKLGLKGYHGNNLDAMYDVLSTL
                     REDLVIYVYNYDTDILGEYGESICRTLKELEGQKDNITLKTVNISRRY"
     gene            1791..2171
                     /locus_tag="VFD79_00515"
     CDS             1791..2171
                     /locus_tag="VFD79_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HZH92557.1"
                     /translation="MKVIFVTLGFISVGIGAIGAVLPVLPTTPFLLLGSYFLAKGSDR
                     FNDWLKGTKLYKDNLESFERNRSMTRRTKTYLMTLSSSMIWLSIFMVDKPYLKAMLVV
                     IDLFKFYYFIFRIKTEEKKEQKEG"
     gene            2174..2905
                     /locus_tag="VFD79_00520"
     CDS             2174..2905
                     /locus_tag="VFD79_00520"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004836804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HZH92558.1"
                     /translation="MSLDRLEKIIETTNNIVFFGGAGVSTESNIPDFRSESGIYATKN
                     KYGYEPEYLLSHSFFRDHQKEFFGYYRENMLYPDAKPNKAHLALGKLEKLGKLKAVIT
                     QNIDGLHQLGGSKNVLEIHGSVHRNYCQSCHESYPLDKITGSDSVPTCDKCGGVVRPD
                     VVLYEEGLDMDVMERAVDYIRMADVLIVGGTSLNVYPAAGLVEYFRGRELVLINKTAT
                     GYDGRATLILRDSIGEVLDYAVRRL"
     gene            2905..4071
                     /locus_tag="VFD79_00525"
     CDS             2905..4071
                     /locus_tag="VFD79_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZH92559.1"
                     /translation="METKELLEQLIDEEKLSKAILSNRRTKDRALPGKVLVNPVTIKG
                     TLMYQFESHVGNKAFHENLEKEDALQRLLDLVSNDFRQMVLKTSIGEYQILVSKKGKQ
                     KIIGNQAAVTKVEKSHNRKKQYIIEEGQPVDFLVHLGVMDSRGKVYKQKYNKFRQINK
                     FLELVDASMEDLMDQEEIRIIDFGCGKSYLTFALYHYLVNMKKKKVNITGLDLKQDVI
                     DLCNRTTEELNYQGLQFICGDIKDHTSGEGVDMVVTLHACDTATDIALEKAVRWGAKV
                     ILSVPCCQHELYDKIKNNNLSGMLRHGIIRERLSSLVTDSIRGSVLEIMGYQVQILEF
                     IDMEHTPKNIMIRAIKKHSPFREEKVEEYKRLKEMWGLEDIFIQEAMKDLLENK"
     gene            4087..>4964
                     /gene="guaA"
                     /locus_tag="VFD79_00530"
     CDS             4087..>4964
                     /gene="guaA"
                     /locus_tag="VFD79_00530"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF000848.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HZH92560.1"
                     /translation="MEREMVLILDFGGQYNQLIARRVRDLNVYCEVHPYTIGLEKVRE
                     MDPKGIIFTGGPNSVYEEKSPSISEEIYDMGIPILGICYGAQLMSYTLGGEVKAATTR
                     EYGKVKLHVEEKSPIFEDVKEKSITWMSHTDYVEKLAEGFHVTATTESCPTAAFENRE
                     KNLYGVQFHPEVQHSEEGNKVLSNFLFNVAGCSGDWKMDEFVENSIAAIKEKIGDKKV
                     LCALSGGVDSSVAAVMIERAVGKQLTCIFVDHGLLRKDEGDQVEEVFTNNYNLNFIRV
                     NAQQRFYDKLKGVTDP"
BASE COUNT         1546 a          993 c         1242 g         1183 t
ORIGIN      
        1 ttcataaagt cagagaccct ggcaactgat gtccagttcg ttgaggacac cggccttgaa
       61 aagcaggacc ttaacggaga agagacggga attgaactta gcaagaatta acagggataa
      121 ataaagcagg gaacaggcta ctcggcctgt tccctgtatt gtatttttga ccctatttgt
      181 atggaatcct gggtgaccaa actgtcatag acatgaagac ctacaccctc cttaattgcc
      241 aaatccagag cattgccaaa ttcagggtcc attagcctgt ttggtgtgaa ggccattggt
      301 cccttcatct ggataagaaa gaatatgttc ccttcgtagc cttcttggac ggccttgatc
      361 atttccatga tatgctttgt accacggaca gttggagcgt ctggaaattt cgacaatcca
      421 tccatttcca gggtgacacc cttgacctca ataaatcctt ccctgtcacc atccctgtag
      481 tagatgtcaa aacgggagtc tccgaacttt tgctcaccct tgatcaggtc aggagtccca
      541 aacccctgga ttttaccctc catcagggca tccatgacaa ccctgttggg aacctgggaa
      601 tccatgttca caagcatatc ctccttgtag actgatatga gggaatacct tgtctttctc
      661 ttggtactta ggccatggtc ctgaaggatt acccttgcac cgggaagaag gagttccctg
      721 cacctgccgg tatttttcac atgggcaatc tcctccctgc cattgatgag gacgtgggca
      781 ataaacctgt tgggcctctt aagaaaggtc ccctccacca tatttgagta tctcatagtt
      841 aaatcaactc catttcaatc attcaatatt aacacaaatt gaatctccag acaatattag
      901 ggtatctaag gactagtact taaaggagga taaataacat gagacaattg acaaagatca
      961 ccattctaac cctggtcatg gcactgctcc ttataggatt tgcaggatgt tcaggtcttg
     1021 atgtggtggt ggaagagcca gaagctccag gggttgagaa ccctgcaagt gaagaaccac
     1081 aggagctagt tgaggaggca atagctgagg atggctatta tacatcacct gaagaggtgg
     1141 ccctttatct tcacacctac gccaggcttc cggataacta tataaccaaa gaggatgcaa
     1201 tggaccttgg atgggatgcc cagaagggta acctttggga tgtaacagac aggatgagca
     1261 tcgggggaaa caggtttgga aacagggaaa gactccttcc tgaagcagag ggcagacaat
     1321 actacgaggc cgatgtaaac tacgaaggtg gctacagggg agctgaaagg cttgtctatt
     1381 caaatgatgg acttatattc tacactgggg atcactacga atcctttgag cagctttact
     1441 aggaggattg aacatgaaga ttatactgat agatgcaaag aagatgagag acagggactt
     1501 tcacgactac ttcaggagga agctgggcct aaaaggctac catggcaaca atctggatgc
     1561 aatgtatgac gtcctatcaa cattgaggga ggacctggtt atatatgtct acaactatga
     1621 tacggacatt ttgggtgaat acggggaatc aatatgcagg accctaaagg aacttgaagg
     1681 ccaaaaggac aacataacat taaaaaccgt taacatttcc agaaggtatt gaattaagag
     1741 tagaaaagaa atatattatt aacagacaag agaagtaaga gaggaataat atgaaggtga
     1801 tatttgtaac tttgggtttt atttcagtag gaataggggc tataggggcg gtgctgccag
     1861 tgcttcccac tacgcctttt cttttgcttg gatcatattt tctggcaaag gggtctgaca
     1921 gattcaacga ttggctaaag gggaccaaat tatataagga caaccttgaa agctttgaga
     1981 ggaacaggtc catgacaagg aggacaaaga catacctaat gaccctgtca tcgtccatga
     2041 tctggctgag cattttcatg gtggacaagc cctatctaaa ggcaatgctt gttgtaatag
     2101 acctgttcaa gttctattat ttcatattca ggatcaagac agaggaaaaa aaagaacaaa
     2161 aggaggggta ggaatgtcac ttgaccgatt ggaaaagatt attgagacca ccaacaatat
     2221 tgtatttttt ggaggggctg gagtttcaac tgaaagtaac atacctgact ttcgaagtga
     2281 aagcggaata tatgccacaa aaaacaagta tggctacgag cctgaatacc ttctcagcca
     2341 ctccttcttc agagaccatc agaaggagtt tttcggctac tacagagaga atatgctcta
     2401 cccggatgca aagccaaaca aagcccacct ggcccttggg aagcttgaaa aactgggtaa
     2461 gctaaaggca gtcataacac agaatatcga cggactccac cagctgggag gctcaaaaaa
     2521 cgtccttgaa atacacgggt ctgtccatag gaactactgc caatcctgtc atgaaagcta
     2581 cccattggat aagataacag ggtcggattc tgttccaacc tgtgacaagt gtggtggcgt
     2641 agttcggcct gatgttgtat tatatgagga aggcctggat atggatgtaa tggaaagggc
     2701 agtggattac atcaggatgg ctgatgtctt aatagtcggc ggaacatccc ttaacgtata
     2761 tcctgcagca gggctggtgg aatacttcag gggtcgtgag ttggtcctga taaacaaaac
     2821 agcaacaggc tatgacggca gggctaccct aatactaagg gacagcatcg gagaagtatt
     2881 ggattatgca gttaggagat tgtaatggag acaaaggaac tactggagca gttgatagat
     2941 gaagaaaaac tttcaaaggc aatcctaagt aatagaagga ctaaggacag ggccctgcct
     3001 ggcaaggtct tagtaaaccc tgtcaccatc aaggggaccc tcatgtatca gtttgaatca
     3061 catgttggga acaaggcctt ccatgagaac ctggaaaagg aagatgccct ccaaaggctt
     3121 cttgaccttg tttcaaatga tttcagacag atggtcctta agacctcaat cggtgaatac
     3181 cagatacttg tcagcaagaa aggcaagcag aaaatcatag ggaaccaggc agcagtgacc
     3241 aaggtggaga agagccacaa caggaagaag cagtacatca ttgaagaagg tcagccggtt
     3301 gacttccttg ttcacctggg tgtcatggac agtcggggaa aggtgtacaa gcaaaagtac
     3361 aataagttca ggcagataaa caaatttctt gagcttgtgg acgcatccat ggaggacctt
     3421 atggaccagg aggagataag gatcatcgac tttgggtgtg gaaagagcta cctgaccttt
     3481 gccctttacc actatcttgt taacatgaaa aagaaaaagg tcaatatcac aggtcttgac
     3541 cttaagcagg atgtcattga cctttgcaac aggacaacag aggagctaaa ttaccaggga
     3601 ctccaattta tctgtggaga tataaaggac cacacatcag gagagggtgt ggatatggtt
     3661 gttaccctac atgcctgcga cacggcaacc gacatagcac tggaaaaagc tgtaaggtgg
     3721 ggagcaaaag taattttatc tgtaccttgt tgtcagcatg agctttatga taaaatcaag
     3781 aataataatc tttcaggaat gctcaggcat ggaataatca gggagaggtt aagctccctt
     3841 gtaacagact ccatcagggg ctcggtcctt gagataatgg gctaccaggt ccagattttg
     3901 gagttcatag acatggagca taccccaaag aacatcatga tcagggctat taaaaagcat
     3961 tcacccttca gagaagagaa ggttgaggaa tacaagaggc taaaggaaat gtggggtctt
     4021 gaggatatct ttatccagga ggccatgaag gatctattgg aaaacaaata aacgggagga
     4081 atgtcaatgg aaagggaaat ggtacttata cttgacttcg gtggtcagta caaccagctg
     4141 atagcaagaa gggtaaggga tttgaatgtg tattgcgagg tacaccctta cacgatcggt
     4201 cttgaaaagg tcagggaaat ggatccaaag ggaatcatct ttacaggtgg acccaacagc
     4261 gtatatgagg agaaatcccc atcaataagt gaagagattt atgatatggg cattcctata
     4321 ctgggaatct gctatggggc acagcttatg tcctataccc ttgggggaga ggttaaggct
     4381 gccaccacaa gggaatacgg aaaggtcaag ctgcatgtgg aggagaagtc gccaatattt
     4441 gaggatgtta aggaaaaatc cataacctgg atgagccata ctgattatgt tgagaagctt
     4501 gcagagggct ttcatgtaac tgccacaacg gaatcatgcc caacggcagc ctttgaaaac
     4561 agggagaaaa acctttacgg ggtccagttc cacccagagg ttcaacattc agaggaaggt
     4621 aacaaggtcc tttcaaactt cctcttcaac gttgcaggct gcagtggaga ctggaagatg
     4681 gacgagtttg ttgaaaattc aatagccgca atcaaggaga agattgggga caagaaggtc
     4741 ctttgcgcac tttcaggagg agttgactcc tcagttgcag cggtcatgat agaaagggct
     4801 gttggaaaac agcttacctg catattcgtt gaccacggtc tgcttaggaa ggacgaggga
     4861 gaccaggttg aagaggtgtt tacaaacaac tacaacctga acttcatcag ggtaaacgcc
     4921 cagcagagat tctacgacaa attgaaggga gtaacagacc ccga
//
LOCUS       DASRQF010000020         4875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_112433, whole genome shotgun sequence.
ACCESSION   DASRQF010000020 DASRQF010000000
VERSION     DASRQF010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112433"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="VFD79_00535"
     CDS             complement(<1..757)
                     /locus_tag="VFD79_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZH92561.1"
                     /translation="MSNVHELGFLKEKIQELKDEGVYRKLPVMGSASDAEVELNGRKV
                     INLSSNNYLGFANNPRMKKAAIDAVEKYGVGSGAVRTIIGNMDIHEKLEETLAKFKRE
                     EAAFIYQSGFNCNAGTIQAITEAGDLIISDELNHASIIDGARLSKAAKTIYKHSDMDS
                     LEAVLKENKDKYRNMLIITDGVFSMDGDIAKLPEIVDLAEKYGAMTYVDDAHGSGVLG
                     ESGRGTVDHFGLHGRIDFTIGTLSKAIGVIGGYV"
     gene            complement(811..1824)
                     /gene="queG"
                     /locus_tag="VFD79_00540"
     CDS             complement(811..1824)
                     /gene="queG"
                     /locus_tag="VFD79_00540"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HZH92562.1"
                     /translation="MIKARINDIAKHLDIDMIGFTHSGPLERIRPQLQNRVDKERATE
                     FEERNLDLRLDPRLTMENCKSIIVVALSYNREKKDTKKARVKGSLSKSSWGRDYHYVL
                     KGKMDLLVNEMKKEFAFNHMSFCDTGPLVDRELAYRAGIGYYGKNCSIINPEYGSFIF
                     IGYILTDLEIECEDKNMESLCGDCTLCSLACPTGALEGAGILNPKKCVSYLTQTKEPI
                     PEDLSSGMGIKIYGCDTCQLVCPKNKYVKISHHGEFDPLKTGGIVDIEELINMSNKEF
                     HVKYGDMAGSWRGKTVLLRNSIIALENMGTHCHKDLIETLERKNIKLLKPYTSRILKG
                     MVE"
     gene            1931..3040
                     /gene="rodA"
                     /locus_tag="VFD79_00545"
     CDS             1931..3040
                     /gene="rodA"
                     /locus_tag="VFD79_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002836358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HZH92563.1"
                     /translation="MFNLRKKAFKRFDFLLLITVVLLSIYGLVVIYSATLSLENIRFV
                     RTQGISTLIGFVAILFLVFFDFQFLGKLYIPIYIVSVGLLLAVLFMGVGDDQWGARSW
                     LYIGSFGFQPAEFVKVGLIISLAKYMDNNKDDINSPFVLFKILAFAFFPVGLILLQPD
                     AGTAMVFIFFIAAMLFVAGVKWKYIGIALAMGLMSLPILWSRLDPFQKDRIFNFLDPE
                     RDTSNTGFQAIQGRIAIGSGKIFGRGLFEGTQTQFNYIPEKQTDFIFAVLAEELGFIG
                     GSLLILLYFIMINRIIRIARNSPDKFGSFMSVGFAGMFLFHIWENIGMTIGLMPITGI
                     PLPFMSYGGTFQLINLISIGIVLSVGLHREGLSFE"
     gene            3131..3475
                     /locus_tag="VFD79_00550"
     CDS             3131..3475
                     /locus_tag="VFD79_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZH92564.1"
                     /translation="MNIQFKKGVLELCVLTLLNRKDYYGYELVENISTFIKISEGTIY
                     PLLRRFRSDGYVTTYLEESQEGPPRKYYRLTDKGKEVLIILQEEWENFVNGVNLLLRG
                     VDDEQEGIFEHA"
     gene            3444..4019
                     /locus_tag="VFD79_00555"
     CDS             3444..4019
                     /locus_tag="VFD79_00555"
                     /inference="COORDINATES: protein motif:HMM:NF019618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HZH92565.1"
                     /translation="MNRKEYLNTLKSYLSGLREGELREILLDYEEHFNIGREKGKTDD
                     EISLELGTPKEVAESILSTIHADANNTNVTSQDKNPNDSARRFLIFLLLLGLNLVFIL
                     GPVAAVIGIIIGIFGTGIGFVVGGVGLIFGAPFAGLVSGIAPGIITSVSFGIGLGALG
                     GLVFILGIVLSKLFYRLMSMYIDWNRRIING"
     gene            4031..>4875
                     /locus_tag="VFD79_00560"
     CDS             4031..>4875
                     /locus_tag="VFD79_00560"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HZH92566.1"
                     /translation="MDLKRLSIVLAGVVLIAFGVGLYSLFYIDNFRFGDISENRSLEI
                     HDNGETVKIDSEGIRVVEDGKSVTISWDGIEVIENGKKTLVGLRDINFFERIDIFSSL
                     TSYEANEAESMQLAEEVDLISIYTTFADTRIVQTDSDRLMVSLKGRYKSNRQVSLNLQ
                     DLGDSIKISMTPETGSYTVSQSDLLLEVLIPENFNEDLEFTSSSADLSSDSLRLKKVK
                     VESSSGRIQIDEVHAQESRITASSGDIRIPMITGDISIASSSGDVTVGPVEPLDEVKV
                     VTSSG"
BASE COUNT         1342 a          979 c         1012 g         1542 t
ORIGIN      
        1 ccacatatcc tccaattact ccaatcgcct tggaaagagt tccgattgta aagtcaatcc
       61 taccatgtag tccaaagtgg tctacggttc ctcttccact ctctccaagg acacctgaac
      121 catgggcatc gtcaacatag gtcattgccc catatttttc agctaggtca acaatttccg
      181 gaagctttgc tatatcccca tccatgctga ataccccatc agttatgatc agcatgtttc
      241 tgtatttgtc cttgttctcc tttaatactg cctccaggct atccatatca gagtgcttgt
      301 aaatagtttt tgcagctttg gaaagtcttg caccatcgat tattgaagca tgatttagct
      361 catctgagat tataaggtcc cctgcttctg tgattgcctg aatggtccct gcattgcagt
      421 taaagccaga ttgatagata aaggctgctt cctccctctt gaatttagca agtgtttcct
      481 caagcttttc atgaatatcc atgttgccta tgatggtgcg gacagctcct gatccaaccc
      541 catatttttc aactgcatct atcgcagcct ttttcattct tggattgttt gcaaatccca
      601 gatagttgtt tgatgaaagg tttattacct tcctgccatt gagctccacc tctgcatcac
      661 ttgcagatcc cattacaggc agttttctgt atactccttc atccttgagt tcctggattt
      721 tttccttcag aaatcccagt tcatgtacat ttgacactaa aatccctcct taaaggtaat
      781 tgtttttctt gttttataat gcaacattag ctattctacc atacctttga gtatccttga
      841 agtataaggt ttcaataatt tgatattctt tctttccaag gtctcaataa ggtccttgtg
      901 acagtgtgtc cccatgttct ccaatgcaat tatagagttc cttaaaagta ccgtcttacc
      961 cctccagctt cctgccatgt ctccgtactt cacatgaaac tctttatttg acatgttaat
     1021 caattcctca atgtcaacta tgccaccagt ctttaatgga tcgaactctc catgatgaga
     1081 tattttgaca tacttgtttt tgggacacac aagctgacat gtgtcacacc catatatctt
     1141 tatacccatt ccggatgaca aatcctcagg tattggctcc ttggtctggg tcaggtatga
     1201 tacacatttt ttagggttga gtatacctgc cccttccagg gctccagttg gacaggcaag
     1261 ggagcatagg gtacagtcac cgcataaact ttccatattt ttatcctcac attcaatttc
     1321 caggtcggtt aaaatatatc caatgaaaat gaaggaacca tattctggat taatgatgct
     1381 gcagttcttg ccgtaatatc caatcccagc cctgtaggca agctccctgt caacaagtgg
     1441 cccggtgtca cagaatgaca tatggttgaa agcgaattct ttcttcatct catttacaag
     1501 caaatccatc tttcccttaa ggacataatg ataatccctt ccccatgaag acttggaaag
     1561 gctccctttt actctggctt tcttggtgtc ctttttctcc ctgttatagg agagtgcaac
     1621 aacaattatg gatttgcaat tctccatggt aagccttggg tccagtctaa ggtctagatt
     1681 cctttcctca aattctgttg ccctttcctt gtccactcta ttttgcaatt gtggacggat
     1741 cctctccaga ggccctgaat gagtgaatcc aatcatgtct atatcaaggt gtttagcaat
     1801 atcatttatc ctagccttaa tcatcccaat ccctccgttt ctaattatat caaataatat
     1861 acaataaacc cactacttga aaaaaccata gtatgatata atattccttg atgcaaagga
     1921 gtagatgatt atgtttaatc ttagaaaaaa agctttcaag agattcgatt ttctgttgct
     1981 tataacagtt gttcttttga gcatatatgg actggtagtt atatattcgg caaccttgag
     2041 cctggaaaac attaggtttg taagaaccca gggaatttca accctaattg gatttgtggc
     2101 aatacttttt ctggtgttct ttgacttcca atttttaggc aagctataca tccccattta
     2161 catcgtctca gttggattgc ttctggcagt acttttcatg ggagttgggg atgaccagtg
     2221 gggggcccgt tcatggcttt acattgggtc atttggattc cagcctgctg agttcgtcaa
     2281 ggtaggtttg ataatttccc tggccaagta catggacaac aacaaggatg atatcaattc
     2341 tccctttgtg ctgtttaaga tcctggcatt tgcatttttc cctgtgggct tgatactgct
     2401 tcagccagat gcaggtactg caatggtctt tatcttcttt attgcagcaa tgctctttgt
     2461 ggcaggagtt aaatggaaat acattggaat cgctcttgca atggggctaa tgagccttcc
     2521 tatactttgg tcaagacttg atcccttcca aaaggatagg atattcaatt ttctggatcc
     2581 tgaaagagat acttcaaata ctggttttca agccatacaa ggaaggattg ccattggaag
     2641 tggcaagata tttggaagag gtttgtttga gggtacccaa acccagttta actatattcc
     2701 agagaagcaa accgacttta tttttgcagt acttgcagag gagttaggct ttatcggcgg
     2761 aagcctgcta attctcctgt atttcataat gatcaacagg ataataagga ttgcacgtaa
     2821 cagccctgac aaattcggat catttatgtc tgttggattt gccggaatgt tcctattcca
     2881 catatgggaa aacataggaa tgaccatagg cctgatgcct ataactggta ttccccttcc
     2941 ctttatgagc tatggaggaa cctttcagct gataaacctc atatccatag gaatcgtatt
     3001 aagtgttgga ttgcacaggg aaggacttag ctttgagtag ataggagaat ttttctccta
     3061 tttttttaat ttagtacctt gcattgtaag gtagtgtgtg atatattata gccgtgaaag
     3121 gagactagcc atgaatatac aatttaaaaa gggagttctt gagctttgtg ttctcacttt
     3181 actgaacaga aaagattatt atgggtatga attggttgaa aatatttcca ctttcattaa
     3241 gatttctgaa ggcacaatat atccactcct cagaagattc agaagtgacg ggtatgttac
     3301 aacctattta gaggaatccc aggaaggccc tccaagaaag tactacagac ttacagacaa
     3361 gggcaaggaa gtactcataa tacttcagga agaatgggag aattttgtga acggagtcaa
     3421 tttactgtta aggggtgtag acgatgaaca ggaaggaata tttgaacacg cttaaatctt
     3481 atctttcagg cttaagggaa ggggagcttc gagagatact gcttgattac gaagagcatt
     3541 tcaatatcgg acgtgaaaaa ggtaagacag atgatgaaat atccttagag ctgggaacgc
     3601 caaaggaagt ggcagaaagc atattaagca ccatccatgc tgatgcgaac aatactaatg
     3661 tcacctccca ggataagaat cccaatgaca gtgctagaag attccttata ttccttctgc
     3721 ttttggggct caatctggtt tttatccttg gaccagtggc ggcagtaata gggattataa
     3781 tcgggatttt tggaacggga attggatttg tggtcggtgg agttggttta atcttcggcg
     3841 ctccctttgc aggcttggtt tcaggaatag ctccgggaat tattacatca gtttcctttg
     3901 gtattggact aggtgccttg ggagggttgg tctttattct tgggatagtt cttagcaaac
     3961 tattctacag gctgatgtcc atgtacattg attggaatag aagaattata aatggttagg
     4021 gggcgagaaa atggacttaa aaagactatc aatagtactg gcaggagttg tactgattgc
     4081 ttttggtgta ggtctatatt cactttttta catcgataat ttcagatttg gggatatctc
     4141 agaaaacaga tctttggaga tacatgacaa tggagaaact gtgaaaattg attctgaagg
     4201 tattcgggtg gttgaggacg gaaaatctgt gacaattagc tgggatggga ttgaagtaat
     4261 tgaaaatgga aagaaaacct tagtaggctt aagggacatt aatttctttg aacgaataga
     4321 cattttttct agccttacct catatgaggc caatgaagca gaaagtatgc agctggcaga
     4381 ggaggtcgat ttgatttcaa tctacactac ctttgctgac acgagaatag ttcaaacaga
     4441 ttcagatagg ctgatggttt cactaaaggg aagatacaag tccaacagac aggtatccct
     4501 taatcttcag gatttgggag acagtatcaa gatttcaatg acacctgaaa ccggtagcta
     4561 tacggtctct caatcagatc ttctcttgga ggtccttatc cctgagaatt tcaatgagga
     4621 ccttgagttt acaagctcat cagctgattt gagttcagac tccctaaggc taaagaaggt
     4681 caaggttgag tcaagctcag ggaggatcca aatagatgag gtccatgccc aggaatccag
     4741 gattacagct tcatctggtg acattaggat accaatgatc actggtgaca tttcaattgc
     4801 aagcagctct ggtgatgtta cagttggacc tgttgaaccc cttgatgaag tcaaggttgt
     4861 cacaagttct ggcga
//
LOCUS       DASRQF010000021         3078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1101878, whole genome shotgun sequence.
ACCESSION   DASRQF010000021 DASRQF010000000
VERSION     DASRQF010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101878"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VFD79_00565"
     CDS             complement(<1..664)
                     /locus_tag="VFD79_00565"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HZH92567.1"
                     /translation="MEQKRENWGSRLGFIMAAAGSAVGLGNIWRFPYTAGENGGGAFI
                     LIYLAFVFVIGISVMIAELSLGRSANTAAVGAFKKYDNRWTFVGVMGVLSAFLIMGFY
                     PVVGGWSLAYIFKSFTGLVSDPSAVPAIFDAFIANPIEPIGWMLVYLFANVAIVAKGI
                     SGGIEKAGKVLMPMLFGLLVLLSIRSVTLPGAGAGMEFLLKPDFTKVTGETFLAALGQ
                     AFF"
     gene            1057..2667
                     /locus_tag="VFD79_00570"
     CDS             1057..2667
                     /locus_tag="VFD79_00570"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZH92568.1"
                     /translation="MEKLRFEEMQLSEEIKKAVKTMGFEEASPIQSQAIPLLMEGKDI
                     IGQAQTGTGKTAAFGIPILEKVNTDDKSVQALILCPTRELSIQVSQEISKLGKFKKGM
                     NILPVYGGQPIDRQIKALKKGVQIVVGTPGRVIDHINRRTLKLGGLSMMILDEADEMF
                     DMGFRDDIELVINKIPEERQTIFFSATMPPEIRRFAEKYQTNPEFIKVVHKEMTVPRV
                     EQAYFELKQHMKTEILSRLIDINNPKLTIVFCNTKRKVDELAGELQSRGYSVDALHGD
                     LKQNQRDNVMGKFRKGTIDILVATDVAARGLDVDDVDMVINYDIPQDEEYYVHRIGRT
                     ARAGRDGAAFSFVSGREIYKLKDIQKYTKTKIERRDLPTLKDIKEQHTDTILGKIREA
                     IESEDLAKHSSMIDTLLQEEYNSFDIASALLKMYLEENKLMENHQELDMVDTGKKYKS
                     KGTSTRVHVNVGKQQGISPRHLLSTIFQETGLSKKLIGNIDVYDKFTFMEIEGNYADV
                     VMNGLNGKTIKGHKIRVERASEKKKKRK"
     gene            complement(2699..>3078)
                     /locus_tag="VFD79_00575"
     CDS             complement(2699..>3078)
                     /locus_tag="VFD79_00575"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HZH92569.1"
                     /translation="DGMLPLYVSGAAVVLGHNYPFFMGFKGGKGTASTVGMLFSINIV
                     LGFLSILIILVLTIITDYIIYGTLGLLILLVWATVYFDFGLACLIISLLLSAQSIYKH
                     IPNFKRVFRKEEKGLRETIKKQP"
BASE COUNT         1056 a          621 c          694 g          707 t
ORIGIN      
        1 agaagaatgc ctgccccaat gctgcaagga aagtctcccc tgtaaccttt gtaaaatctg
       61 gcttaagcaa aaactccatt cccgcgcccg ctcctggaag agttacacta cggattgaaa
      121 gcagcaccaa aagaccgaac aacataggca taagcacctt gcccgctttt tctataccac
      181 cagaaatacc ctttgccaca atggcgacat tggcaaatag gtataccagc atccatccta
      241 taggctcaat agggttagca ataaaagcat caaagattgc tggtactgca gatgggtcgc
      301 tcaccaaacc tgtaaaagat ttgaatatgt aggcaagtga ccatcctcca acaacaggat
      361 agaaacccat tatcaagaaa gctgacagga cgcccataac acctacaaag gtccacctgt
      421 tgtcatactt cttgaaggct cccactgctg cagtattggc acttctacca agtgaaagct
      481 ctgctatcat gacactgata ccaataacaa atacaaatgc aaggtagatt agaataaatg
      541 ctcctccacc gttttcacct gcagtatatg ggaatctcca gatattcccc agacctactg
      601 ctgatcctgc cgcggccatt ataaagccaa ggcgcgaacc ccaattttct cttttctgtt
      661 ccataaaatc cctcctaaat aatagttagt aactaaatga tagcgaatgt tgcaaacgtt
      721 ttcagaacac acctccctgc ggttgatctt taaaataaaa gtgggtatgc tcaagcaggt
      781 cagtcagatt tattatgtag tgttgcttaa gcttatcccc taaaaaacca tgtttaagga
      841 acagtctaac acactggtaa attaattaca agtcattttc ggaattgtca aataattaac
      901 caataatgat aatcaatgca attcgacaaa gtgtagcagc gtggaaaaac aaagggaaca
      961 aaaagaaata ttaaaaaaac ttaaaaaaca atttgctttt ttcatatgta gcatatatac
     1021 ttaataaggt taacaacaag gaggaaaatt attttaatgg aaaaactaag atttgaagaa
     1081 atgcaattgt cagaggaaat caagaaggct gtaaagacca tgggtttcga ggaagcctca
     1141 ccgatacagt cacaggccat tccattgctt atggagggca aggacataat cgggcaggca
     1201 cagacaggaa cgggtaaaac ggctgccttt ggcattccca tattggagaa ggtcaataca
     1261 gatgataagt cagtgcaggc actgatactg tgtcccacaa gggagttatc aatacaggtg
     1321 tcccaggaaa tcagcaagct gggtaagttc aagaagggca tgaatatcct gccggtctac
     1381 ggaggccagc ctattgacag acagataaag gctctgaaaa agggagttca gatagtggtg
     1441 ggtacccctg gaagggttat tgaccacata aacaggagaa cattgaagct gggcggattg
     1501 agtatgatga tacttgacga ggcggatgag atgtttgaca tgggattcag ggatgacatt
     1561 gagcttgtca taaacaagat acctgaggag agacagacaa tattcttttc agccacaatg
     1621 ccaccggaaa tcaggaggtt tgctgaaaaa taccagacta acccggaatt cataaaggtg
     1681 gttcacaagg aaatgactgt tcccagagtg gagcaggcat actttgagct aaagcagcac
     1741 atgaagactg aaatactaag tagacttata gatataaaca atccaaagct cacaattgtt
     1801 ttctgcaaca ccaaaagaaa ggttgacgag ctggcaggag agcttcaatc caggggatat
     1861 tccgttgatg cacttcacgg tgacctgaag cagaatcagc gtgacaatgt aatgggcaag
     1921 ttcagaaaag gaacaataga tattctggtt gcaactgatg ttgccgcaag gggattggat
     1981 gttgacgatg ttgacatggt aataaactac gatatacccc aggatgagga gtactatgta
     2041 caccggatcg gcaggaccgc aagggccggt agggacggag cagcctttag ctttgtttca
     2101 ggcagggaga tctacaagct taaggacatc caaaagtaca caaagaccaa aatagagaga
     2161 agggaccttc caaccctgaa ggatatcaag gagcagcata ctgatacaat tcttgggaag
     2221 ataagggaag ccatcgagtc agaggatctt gcaaagcact cctcaatgat agataccctg
     2281 cttcaagagg aatacaattc ctttgatata gcaagtgccc tgttgaagat gtaccttgag
     2341 gaaaacaagc ttatggaaaa ccaccaggag cttgatatgg ttgacacagg caagaagtac
     2401 aagtcaaagg gtacaagcac aagggtacac gtaaacgttg gaaagcaaca gggaataagc
     2461 ccaagacatc tcttgtctac aatattccag gagactggat tgagcaagaa gctaattgga
     2521 aacattgatg tgtatgacaa gttcaccttt atggagatag agggaaacta tgcagatgtg
     2581 gttatgaacg gacttaacgg taagacaata aaaggccaca aaataagggt tgaaagagcc
     2641 agcgaaaaga agaagaaaag aaaataatcc ataaaaattt acagccacag gacaagtcct
     2701 atggctgttt tttgatggtt tcacgtagtc ccttctcttc tttgcgaaaa actctcttga
     2761 aattcgggat atgcttgtat atggattgtg ctgacagtaa aagggaaatt atcaggcagg
     2821 ccagaccaaa atcaaagtag actgtcgccc aaacaagaag aattaggagt cccaaggtcc
     2881 catagatgat gtagtcagtt atgattgtaa gaaccagtat tataagaatg cttagaaaac
     2941 ccagcacaat gttgattgaa aagagcattc ccacggtgga agcggttccc ttgccgcctt
     3001 taaatcccat gaagaacgga tagttgtgtc ctaaaacaac agccgccccg cttacataga
     3061 ggggaagcat gccatctg
//
LOCUS       DASRQF010000022         7552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_974638, whole genome shotgun sequence.
ACCESSION   DASRQF010000022 DASRQF010000000
VERSION     DASRQF010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974638"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="VFD79_00580"
     CDS             complement(<1..1126)
                     /locus_tag="VFD79_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92570.1"
                     /translation="MGFTGERLLEEDRNISVFMEYMKKRDEESLDLAKKALRNIKSYS
                     STRRDGKGYRIRIKCKNEEKTYSYSFPYIYGDLMDIIQENVNLNQGRTGYLENTGGFM
                     LNYMTQRGLDSVGFAMDESAWERSIFSNFPVYRREYLKRELSPLVVGKGLDRNLDRLI
                     WIVEEYEKEKDFLQDHLRDLNMDVFILVPEELNLNLEDFKRNPLLSRTFVTVDREFRF
                     LLLSSRDSYDPEITRVPEGLEVLETESAIFRVKMDKCRDNNGFQYTEGGHYFVETLRE
                     YENNSDLEYKDSFLRKYYDSFQPNNRRDNYLWEEGVECFPMDHGWLYDPWRSPANPPL
                     RKRRFETRKGGNHNIGPNTEEFGQAEFRRLIGAYEALKDGG"
     gene            complement(1217..1942)
                     /locus_tag="VFD79_00585"
     CDS             complement(1217..1942)
                     /locus_tag="VFD79_00585"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZH92571.1"
                     /translation="MSVNDGDGIRTLIFMAGCPLKCQWCSNPESQGSPKDGLGRLGFV
                     REYSLDELMEIIERQKIFYRYSGGGVTFSGGEATMQLEILEEISSLLYDKAIDMAMET
                     SGYFEFQSVKGILDRMNLIFVDLKVFDGDDHRKFTGRDNALILDNIGKLGELNYPLVV
                     RIPLIVGVNTHFENISATADFIGKHIRDPKVELLPYHSFGDEKYLDLGLELPSREFKT
                     PSHDTVEEISRHFRSKGIQVVKY"
     gene            complement(1972..4338)
                     /locus_tag="VFD79_00590"
     CDS             complement(1972..4338)
                     /locus_tag="VFD79_00590"
                     /inference="COORDINATES: protein motif:HMM:NF014900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HZH92572.1"
                     /translation="MTRTRNEEARLNYIESKPSISYERARLWTESHKRTEGESVPIRR
                     AKAFRDTCRNLDVHIFDGEIIVGAVGEFRKCGILTPEFSWTWVDREMDNFSSRRQDPY
                     EMTDEQREYVRREIFPYWKGKSLEEAFLSRLPKETANVAVDTGIIDNDSKWRQAVGEI
                     TPDYQDVLFTKGFGGIILEAEEKLSGLDPAEANNLEKIDFYNSIILTSKGIIELANRY
                     ADEAKRIAQSQQDAARKEELLEIERVCRKIPEFPPESFHEAIQFVWFTQLGGILSENP
                     LALNIGRFDQFMYPYYLSDRDGGMADDRIQELIEALWIKLSEWVWTISANTAEFFAGY
                     NQFQNLTVGGKTREGRDATNELSYMCLKATDSVRTHQPGLSVRIHQDCPDSFMEAVTD
                     LVSKGTGFPAIHSDSAGYQMLLNAGYEPEDARDWNNCGCVVPHFRKTGEWTSAVNINF
                     TAALEFALNKGVSRVHGRRMGVTDKSPEDYKSYNEVEEAFYREFDKLVYHSVIATLTA
                     QRLHKEMVPRPFLSSCMEDCIEDGVDLVNGGAKYNIGPVLTGIGLAVTSNSLAVIKKL
                     VFEDRLTTMEELSRAMDANWEGYEGLRDLALQVPKYGNDLEYVDEIAIKLANHYYKVG
                     HGFKDINGNSFNTAFMGISNYLPTGKVVGATPCGRKAGEPLSEGVSPFAGTDRSTPLA
                     AMRSAAKLNQDIHSGGTLLNLRLNQSLVDSKRGRSNLGAMIQSFFSLGAFHVQFNTIS
                     TEVLREAQEEPEKYKDLLVRVAGYSTQFVNLSREMQDAIIARTAHEGY"
     gene            complement(4427..4978)
                     /locus_tag="VFD79_00595"
     CDS             complement(4427..4978)
                     /locus_tag="VFD79_00595"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HZH92573.1"
                     /translation="MTDPSTNIGIVLKRVRTERGLTLEGTSELTGVSKAMLGQIERGE
                     SNPTISTLWKISTGLKLSFSELLEPKGEEHLGVVNFEDQDPVLESEGKMMLYNVFPFN
                     PVSGFEYFYIKLLPSAFHKSSPHSSSSREYIVVTKGRLIVQVDDESYQIKAPAAFSFR
                     ADREHSYSNPFVEPVEFQNIIKY"
     gene            5143..5709
                     /gene="lepB"
                     /locus_tag="VFD79_00600"
     CDS             5143..5709
                     /gene="lepB"
                     /locus_tag="VFD79_00600"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZH92574.1"
                     /translation="MSADNSNLSLNSKLIIFFVIAAIMFTGIIISFDFIEISGDSMMP
                     TLASGDSVLIWSLPYVPGMPDEKLDYGDIIVISGPDSMDNVLMIKRIIGFEDDVIEII
                     EGQVYRNGQLLTEGYITGMTLPLNLQKDPGKMPEWDEEYTYTVPENHVYLLGDNRLVS
                     TDSREYGALPISDIIGRMIFPRKVLQTP"
     gene            complement(5684..7210)
                     /gene="cls"
                     /locus_tag="VFD79_00605"
     CDS             complement(5684..7210)
                     /gene="cls"
                     /locus_tag="VFD79_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HZH92575.1"
                     /translation="MLLKKINYRITLIGIIIVAQLVWGILFLLRILDYSEWISSALSI
                     FSILVVLFLNMKEENASYKIGWIILIMALPVFGGLFYLVFGNKRPSRFMRTRLANEHE
                     KVLPLLIQDSETAERLEAIDQRATGTSRYVYSQSDYPLYGDTMTKYYPLGEEMFDAML
                     QEMEKAQSYIFFEYFIIEQGVMWDRMLEVLVRKVREGLDVRMIYDDVGSMFLLPGDFA
                     RRMEDLGIRCMAFNRFRPVLSVVMNHRDHRKILVIDGHTAFTGGINLADEYINVKERF
                     GHWKDTGIMLKGVGVWSFTVMFLEMWNAFRDDRGDPKDYRYKGEEAIKHDSGGYVQPF
                     SDSPLDNEPLGENIYIEILSQARDYVYIFTPYLIIDNELQTALCMAAKRGVDIRIVTP
                     GIPDKKLVFRITRSNYLPLLRAGVRIYEYTPGFMHAKSYVCDDKIGVVGTVNMDYRSL
                     YLHFECGVILYRNKSLIRLREDSLDTIQKSTEIFIEDIDKGFLSRVFDAILRVFAILF
                     "
     gene            complement(7214..>7552)
                     /locus_tag="VFD79_00610"
     CDS             complement(7214..>7552)
                     /locus_tag="VFD79_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92576.1"
                     /translation="LYGTGMGPLRSAVDKGELVAIVVDGNGAKILREVFPDNVIVVGV
                     RAPLEECISRMEGRGESLIQPRVDSHGEEMEVMLHLAHMTIENSRDSWWKSRIIVEGL
                     GEVLKKQTGD"
BASE COUNT         1974 a         1954 c         1370 g         2254 t
ORIGIN      
        1 agccaccatc ctttaaagcc tcatatgcac ctattagcct tctaaactca gcctgtccaa
       61 attcctctgt gtttggccca atattgtggt ttccaccctt tctggtttca aatcttcttt
      121 tacgcagggg tgggtttgct ggacttctcc aggggtcgta gagccatcca tggtccatgg
      181 gaaaacactc aaccccctcc tcccacaggt aattgtccct tctgttatta ggctgaaagc
      241 tatcgtagta tttccttaaa aaagaatcct tatattccaa atcactgtta ttctcatatt
      301 ccctgagggt ttcaacaaaa taatgccccc cttctgtgta ttgaaagcca ttgttgtccc
      361 tgcacttgtc catcttgacc ctgaatatgg cagactcagt ctccagaacc tccaatccct
      421 ctggaaccct tgtgatttcc gggtcatagc tatccctgga ggataagagc aggaatctga
      481 actccctgtc tacagtcaca aaggttctgc taagaagcgg attccttttg aaatcctcca
      541 ggttcagatt caattcttca ggtacaagga tgaaaacatc catattcaaa tccctaagat
      601 gatcttgtaa aaaatccttc tccttttcat actcctccac aatccagata agtctgtcca
      661 aattcctgtc taaacccttc ccaaccacca agggcgagag ctcccttttc aggtactccc
      721 tcctatatac ggggaaattg gaaaagatgc ttctttccca agcgctttca tccattgcaa
      781 aaccaacaga atccaagcct ctctgggtca tgtaattcag catgaagccg cctgtgtttt
      841 caaggtatcc tgtccttcct tgatttagat ttacattctc ctgaatgata tccataaggt
      901 ccccatatat gtatggaaat gaatagctgt atgttttttc ctcattttta cacttaatcc
      961 ttatccggta gcccttgcca tcccttctag tgctggagta ggactttatg tttctcaagg
     1021 cctttttagc aaggtcaaga ctctcttcat cccttttttt catatactcc ataaaaacag
     1081 aaatattcct gtcttcttca agtagccttt ctcctgtaaa tcccattaag tcaccccctc
     1141 acaactaaaa tatacccatt caggtttaag tgaaaagctc ctggaaaaat ttccgggagc
     1201 ttgttttatg aaaattctag tattttacaa cctgtattcc cttcgatcta aaatggcggc
     1261 ttatttcctc gactgtatca tggcttgggg ttttgaactc cctggacgga agctcaagtc
     1321 caagatccaa gtacttttca tctccaaagg aatggtaggg taacagttcc accttggggt
     1381 ccctaatatg ctttcctatg aaatctgcag tggccgaaat attttcaaag tgggtgttca
     1441 ctcccacaat cagaggtatc ctaaccacaa gaggataatt cagctccccc aatttcccta
     1501 tgttatcaag gattaaagca ttatccctac cagtgaattt tctatggtca tcaccatcaa
     1561 aaaccttcaa atcaacaaat atgagattca ttctatccag aatccctttt acagattgaa
     1621 attcaaaata cccactggtt tccatggcca tgtcaattgc cttgtcatac agtaaggacg
     1681 aaatctcttc aaggatttcc agctgcattg ttgcctctcc tccggaaaag gtaacgcctc
     1741 cacctgaata cctgtaaaat attttctgcc tttcaattat ctccattaac tcatccagac
     1801 tatactccct tacaaatccc agcctaccca ggccatcctt gggactaccc tgactttcag
     1861 gatttgaaca ccactggcat ttaagggggc agccagccat gaaaataaga gtccttatac
     1921 catctccatc attaacagac atattttgtg gttccatgaa aaaacccttc atcaatatcc
     1981 ctcgtgggcg gttcttgcaa ttattgcatc ctgcatttct ctggacaaat tgacaaattg
     2041 ggtgctgtag ccagcaaccc taaccagaag atccttgtat ttttctggtt cctcctgggc
     2101 ttctctcaat acttctgtgg atatggtatt gaactgaaca tggaaagctc ccagggagaa
     2161 aaatgattgt atcatggcac caaggttact cctcccccgc tttgaatcaa caagtgattg
     2221 gtttaatcta agattgagaa gggttccccc tgaatgtatg tcctggttca gcttagctgc
     2281 agacctcatg gcagccaggg gagtgcttct atccgtgcct gcaaagggtg atacaccctc
     2341 agaaagtggt tctccagcct tcctcccaca tggggtagcc cctactacct tgccagttgg
     2401 aaggtaattg gagattccca taaatgccgt attgaagctg tttccattta tgtccttgaa
     2461 accatgacca accttgtagt aatggtttgc aagctttatg gcaatttcat ccacatactc
     2521 caggtcattt ccatactttg ggacctgaag ggcaaggtct ctcagcccct catacccttc
     2581 ccagttcgca tccattgccc ttgacagctc ctccattgtg gtcagtcggt cctcgaaaac
     2641 cagctttttt ataaccgcca atgagtttga tgttacagcc agtccaatcc cagtgagtac
     2701 aggtccaata ttgtactttg cccctccgtt gacaaggtcc actccatcct ctatacagtc
     2761 ttccatacac gaactgagaa atggccttgg taccatttcc ttgtgtaacc tttgtgcggt
     2821 cagggtagct attacagagt ggtatacaag tttgtcaaat tccctgtaga atgcctcctc
     2881 tacttcattg tatgatttat aatcctctgg agacttatca gttaccccca tccttcttcc
     2941 atgtacacgg cttactccct tgttaagtgc aaattccaaa gccgcagtaa aatttatatt
     3001 cacagcagat gtccattcac cggtcttcct gaaatgaggg acaacacagc cacaattgtt
     3061 ccaatctctg gcatcttcag gctcataccc tgcgttcagc agcatctgat aaccggcact
     3121 gtcgctatga attgcaggaa atcctgttcc cttgctcaca aggtctgtta cagcctccat
     3181 aaagctatcc ggacaatcct gatgaatcct tacgctaagc ccaggttgat gggtccttac
     3241 actgtcagtg gccttaaggc acatgtatga gagttcattt gttgcatccc tcccttcccg
     3301 ggtttttcct cccacagtca ggttttggaa ctggttgtat ccagcaaaga attccgctgt
     3361 attggcagaa atcgtccata cccattcaga cagtttaatc caaagagcct caatcagctc
     3421 ttggattcta tcatcagcca tacccccgtc cctgtctgaa agataatatg gatacatgaa
     3481 ctgatcaaat cttcctatgt taagggccaa aggattttcc gacaggattc ccccaagctg
     3541 ggtgaaccac acaaactgaa ttgcttcatg gaatgattca ggtggaaact ccgggatttt
     3601 tctgcatact ctttcaattt caaggagttc ctcctttctg gcagcatcct gttgggactg
     3661 ggcaatcctc ttagcttcat ctgcatatcg atttgccagt tcaattatcc ccttagatgt
     3721 tagtattatg gagttataga agtcgatttt ttcaaggtta tttgcttctg caggatcaag
     3781 tccggacagc ttttcctccg cttccaaaat gattcctcca aaacccttcg taaacagtac
     3841 atcctgatag tctggagtta tctcacccac agcctgtctc cacttggaat cattatctat
     3901 tattccggtg tccactgcaa cgttcgcagt ttccttgggc agtcttgaca gaaaggcctc
     3961 ttcaagtgat tttcccttcc agtagggaaa aatctccctt ctaacatatt ctctttgttc
     4021 atcagtcatt tcatagggat cctgcctcct ggagctgaaa ttatccatct ccctgtcaac
     4081 ccaggtccaa gaaaattcag gagtgagtat tccacacttt ctgaattccc ccactgcccc
     4141 tacaattatt tccccatcga aaatatgaac atccaaattc ctgcatgtat ctctaaatgc
     4201 ctttgccctt ctaatgggca ccgattcccc ttcagtcctc ttgtgggatt cagtccacaa
     4261 cctcgctctt tcatatgata ttgacggttt tgattctatg taattaagtc tagcttcttc
     4321 atttctagtt ctagtcataa caatcactcc ttgtgtaagt ttgttttatt atactatata
     4381 tgttataacg cacgcaacct taaatataaa gagggcattt cgccctctag tattttatta
     4441 tgttctgaaa ttcaactggc tcaacaaatg gatttgaata gctgtgctcc ctgtcggccc
     4501 taaatgaaaa tgctgcaggc gccttgatct ggtagctctc atcatccacc tggactatca
     4561 gcctaccctt ggtaaccact atatactcac gactggagct gctgtgtggt gaagacttgt
     4621 ggaatgcact tggtagaagc tttatgtaga aatactcaaa gcctgatacc ggattaaatg
     4681 gaaagacatt gtagagcatc attttaccct ctgattcaag aaccgggtct tggtcttcaa
     4741 aattcacaac tccaagatgt tcttcaccct ttggttccaa tagctcggaa aaggataact
     4801 tcaagcctgt tgagatcttc catagggttg atatggttgg atttgattcc cctctttcaa
     4861 tctgtccaag cattgccttg ctcacccctg taagctcaga tgttccctcc agggtcaagc
     4921 ctctttcagt tctcaccctt ttaagcacta tacctatatt tgttgatgga tctgtcataa
     4981 taatcacctt ttctttgttt atatgtatta ttctatcact tttccagact tttctccaat
     5041 gcaggaagat ttttaatctt ttctcactct cagttgaata gaatgtcctc caggggtata
     5101 atcaaagtgt gtttaaaatc aaatgattac agggagacca ctatgagcgc agataattca
     5161 aacctaagtc taaatagcaa gctgataata ttttttgtaa tagcagccat catgttcact
     5221 ggaataataa tatcctttga tttcatagaa ataagcggcg attcaatgat gccgacactt
     5281 gcaagcggcg attcagtcct gatatggagc cttccatatg ttccaggcat gccggatgaa
     5341 aagctggatt atggagatat tatcgtgatt tcaggtcctg attcaatgga caatgtcctt
     5401 atgataaaga ggataatagg gtttgaagat gatgtaatag aaatcattga gggccaggtc
     5461 tacagaaacg gccagcttct taccgagggg tacataactg gaatgaccct accccttaat
     5521 ctccaaaagg accctggaaa aatgccggaa tgggatgagg aatacaccta taccgttccc
     5581 gaaaaccatg tatacctgct aggcgacaac aggctggttt caactgacag cagggagtat
     5641 ggtgctctcc ccataagtga catcattggt aggatgatct tccctagaaa agtattgcaa
     5701 acaccctaag gattgcatca aagaccctgg acaggaatcc tttgtcaata tcctcaatga
     5761 atatctcagt gctcttctgg attgtatcca gtgagtcctc cctgagcctt atgagcgact
     5821 tgttcctgta aagtattact ccacactcaa aatgcaggta gaggcttctg taatccatgt
     5881 taacagttcc cacaactcca atcttgtcat cacatacata gctctttgca tgcatgaaac
     5941 caggtgtgta ttcatatatt ctcacacccg ccctcaagag aggcaggtaa ttggacctgg
     6001 tgatcctgaa caccagcttc ttatccggta ttcccggggt cacaatcctg atatccaccc
     6061 ctcgctttgc agccatgcaa agggctgtct gcaattcatt gtcaattatc aggtagggtg
     6121 tgaagatata tacataatct cgggcctgtg acagtatttc tatgtatatg ttctcaccaa
     6181 ggggctcatt atcaaggggt gagtctgaaa atggctgtac atatcctcct gaatcatgct
     6241 taatcgcttc ctctcccttg tatctgtaat ctttggggtc ccccctgtca tcccgaaatg
     6301 cattccacat ctccaaaaac attaccgtga agctccaaac tccaactccc ttgagcatta
     6361 tccccgtatc cttccagtgg ccaaacctct ccttcacatt tatatactca tcagctagat
     6421 ttatcccacc ggtgaatgca gtgtgaccgt ctattaccag gatcttccta tggtccctgt
     6481 ggttcattac aaccgacaag acgggcctga acctgttaaa ggccatgcaa cgaattccca
     6541 ggtcctccat cctcctggca aaatctcccg gaagaaggaa catggacccc acatcgtcgt
     6601 agatcatcct tacatccaaa ccctcccgga ccttcctcac aagaacttca agcatcctgt
     6661 cccacattac accctgctca attataaagt actcaaagaa tatgtaactc tgtgccttct
     6721 ccatttcctg gagcatggca tcaaacattt cctcaccaag ggggtagtat tttgtcatgg
     6781 tatccccata gagagggtag tcgctttgag agtacacata cctggatgta cccgtggccc
     6841 tttggtcaat cgcttcaaga cgttcagcgg tttcactgtc ctgaatcagc aatggaagta
     6901 ccttttcgtg ctcatttgca agccttgtgc gcataaatct ggagggcctc ttgtttccaa
     6961 aaaccaaata gaacaatccc ccaaaaaccg gcaaggccat aataaggatt atccagccga
     7021 ttttgtagga ggcattctcc tccttcatgt tcaggaacag gaccaccaaa atactgaaaa
     7081 tagaaagggc tgagctgatc cattctgagt aatcaaggat cctcaaaagg aaaagtatcc
     7141 cccaaaccag ttgcgcaaca attattatcc ctatcaaggt tatcctgtaa ttaatctttt
     7201 ttagcagcat cctctagtcc cccgtctgtt ttttaagtac ctcacccaat ccctcaacta
     7261 tgattctgga cttccaccag gaatcccttg agttctcaat agtcatatgg gccaggtgga
     7321 gcatgacctc catttcctca ccatgggaat ccacccttgg ctggataaga gactcccctc
     7381 ttccctccat tctggagatg cattcctcca aaggcgcacg gactcctaca actattacat
     7441 tgtccgggaa cacttccctc agtattttgg cgccatttcc atcaaccact atggcgacta
     7501 gctctccttt gtccaccgca cttctcagtg gccccatgcc tgttccgtaa ag
//
LOCUS       DASRQF010000023         6628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_476760, whole genome shotgun sequence.
ACCESSION   DASRQF010000023 DASRQF010000000
VERSION     DASRQF010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6628
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476760"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(307..2016)
                     /locus_tag="VFD79_00615"
     CDS             complement(307..2016)
                     /locus_tag="VFD79_00615"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HZH92577.1"
                     /translation="MYLKKINKKNPDRVYLIIAESFRDKDTKKTKSRTIQSLGYLDEL
                     EKDFDDPIAYFKGVVEEMNNEVQLKKAPIKILFDSQEQLPIGTNNRKNFGYAPFSSIY
                     HTLEIDKFLTSRQRSTKIEASMNNIMKLLVFSRLLSPSSKKKAFEDREQFFEKTNYSL
                     DDVYRSLSFFNDRGNELQKWINDRIKSIYGRDTSLVYYDVTNYYFESDKEDDFRAKGV
                     SKEHRPNPIIQMGLFMDTNGIPITYDLYPGNTNDCLTYRPTFSRMKKEFDLGKVIVVS
                     DKGMSTGDNIQYTLSAKDGFVFSQTARGANRELKEYIRSDDGYVWYGDDYKYKERLYP
                     AEIWVSMTSGKKKKKTIDVKQVVFYSEKYAKKAKMERDKAIEKAKTLIKSPSSYNRST
                     SYGAAGYVKNLSFVKETGEIADASALMLDKEKIREQEAFDGYYVLITSEYEKTASEII
                     DIYRGLWKIEDSFKVTKSDLETRPVYVSREDHIKAHFLTCFIALVLVRILEHITHGKH
                     SITKMVESLRSCTCTNVDQNYYIFDYYDEVLADIGKATNIDFSTKIRTLQQIKKNISE
                     TKN"
     gene            2424..3458
                     /locus_tag="VFD79_00620"
     CDS             2424..3458
                     /locus_tag="VFD79_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZH92578.1"
                     /translation="MIRFESDYSEGAHKKILDMLLETNMEQTPGYGVDHHCENARKYI
                     RACCRDDSLDVHFLVGGTQANTTVIASILKPYQGVVSANTGHINVHETGAIEATGHRV
                     ISMNSEDGKLTSNHVREVYDSHWNDATHEHMVQPGMVYISHPTENGTIYSKNELEEIS
                     RVCQELNLPLFMDGARMGYGLMAEGNDLSLSDIARLCDVFYIGGTKVGALFGEAVVIR
                     SDKLKKDFRYNIKQRGGMLAKGRLLGIQFEALFEDGLYFEISKNAIKTAMMIRDAFRD
                     KGIEFLYDSPTNQQFPILTHQQIEKLSQDYSFQHWARVDHERDAVRFCTSWTTDEANV
                     IKLVQDIKKL"
     gene            3630..5138
                     /locus_tag="VFD79_00625"
     CDS             3630..5138
                     /locus_tag="VFD79_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HZH92579.1"
                     /translation="MRKIGFDPQKYIEEQSKYILERVNNFDKLYLEFGGKLIGDHHAQ
                     RVLPGFDADSKIKLLHKLKDKAEIIICVYAGDIERNKIRGDYGISYDMDVLKQIDELR
                     EYQLKVNSVVITRYNGQPTATQFITKLERRDIKVYTHTAIPGYPSDVDMIVSEEGYGR
                     NPYIETTMPIVVVTAPGPGSGKLATCLNQLYHEHKMDKVAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMMDYFHFERYNEVAVNYNRDLEMFPVIKRIIERITGKESIYH
                     SPTDMGVNRIGFGIVDDQVVRDASKQEIIRRSFATECDYKKGQADEETLNRMRIIMEE
                     LELKREDRAAVGPAREYSQSLKEKEGKNDTPPVIAIELNDGRIITGRGSQLLDATSAA
                     ILNSIKTLANIGDEILLLSPLIIDTIQKLKMESLMGKVGTLDANEALIALSIGAVTNP
                     TAQLAMEKLQDLKGAQAHSTVMLNDNDEQILRRLGVDVTCDPVYPSDNLFYV"
     gene            5239..5721
                     /locus_tag="VFD79_00630"
     CDS             5239..5721
                     /locus_tag="VFD79_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92580.1"
                     /translation="MNWLLYLITILFLAYSYVKDKGKTKKSLSKAWKAFENILPEFLV
                     VIIMVGLMMAVLDPELIVKVIGEDSGWLGVVLASIVGSVTLIPGFVAFPTADILLQGG
                     AGYMQIGAFVSTLMMVGIVTIPVEVKYFGGKITAYRNALAFVFSFFVAYAISLVVNLV
                     "
     gene            5718..6257
                     /locus_tag="VFD79_00635"
     CDS             5718..6257
                     /locus_tag="VFD79_00635"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HZH92581.1"
                     /translation="MIKTIMRYRWVIISMIVMGIVNIADRGLGSRAVGTTMMTLKEML
                     FIIPPIFVLLGLLDTWVPKDTMVRYMGEDSGLKGVILAFFIGSAAAGPLYGAFPVAAI
                     FMRKGVKFSNILIFIGAWSTTKIPMFLFELSALGASFAITRLLVNIPGIIIIAYLLNS
                     LVSREEVETMYLNAERLSD"
     gene            6314..6390
                     /locus_tag="VFD79_00640"
     tRNA            6314..6390
                     /locus_tag="VFD79_00640"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6348..6350,aa:Pro,seq:cgg)
     gene            complement(6407..>6628)
                     /locus_tag="VFD79_00645"
     CDS             complement(6407..>6628)
                     /locus_tag="VFD79_00645"
                     /inference="COORDINATES: protein motif:HMM:NF024052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HZH92582.1"
                     /translation="CEVSKYSADERLQIFRRHMERVLLAVLGGDKEFILKNSKLLDAK
                     NHIYYYYRKERYIFEEMGRVRDYIKESAE"
BASE COUNT         2021 a         1268 c         1426 g         1913 t
ORIGIN      
        1 atgctatcga tggtcgtggc cccagtatcc agttcataga ggctttcaag tatttcatca
       61 agcttgtcag tttggtttag aataacaaac agtgcataca tttaaacact tccttctttt
      121 ttgatggttc aattatatca aatgaaataa tttctatcaa ttttttttaa ttgtccttgc
      181 atttatgaaa acagaagcat ataacttaag ttttgcagca aagtgtcaaa aaaacacctc
      241 gcaaacccac tgttttcagt gatttgcgag gtttcgattt ttgcatattt ttgtagtgaa
      301 acagacctaa tttttagttt ctgaaatatt ttttttgatc tgctgcaagg tccttatctt
      361 tgttgagaaa tcaatgtttg tagcctttcc aatatcagct aatacctcat cataataatc
      421 aaagatgtag tagttttggt ccacgtttgt acaagtacag cttctaaggc tttcaaccat
      481 ctttgtgatg ctgtgcttgc catgagttat gtgctctagg attctaacca gcacaagtgc
      541 gatgaagcat gtcagaaaat gtgctttaat gtggtcttct cttgatacat atacaggtct
      601 tgtttccaag tcgctcttgg ttactttaaa tgaatcttca attttccata aacctctgta
      661 aatatcaata atctcacttg ctgttttttc atattcactt gttatcagaa catagtaacc
      721 atcaaaggcc tcttgttccc taatcttttc tttatcaagc attaaggcac ttgcatctgc
      781 tatctcaccg gtttccttca caaagctaag gttcttgaca tagcctgctg caccatagga
      841 ggtagaacgg ttgtagcttg atggggactt aataagtgtt tttgcttttt caatagcctt
      901 atccctttcc atcttagctt tttttgcata tttctcactg tagaaaacta cctgtttcac
      961 atcaatagtc tttttcttct ttttgccaga agtcatacta acccatattt cagcgggata
     1021 aagtctttcc ttgtacttgt aatcatctcc ataccataca tatccatcat cagatcgaat
     1081 gtattctttc aactccctgt tagcccctct agctgtttga gaaaatacaa agccatcctt
     1141 agcagagagg gtatattgaa tattatctcc agtagacatg cctttgtcgg agacaacaat
     1201 gaccttgccc agatcaaatt cttttttcat tcttgaaaaa gtaggtctgt aagttaaaca
     1261 atcattggtg tttcccgggt acagatcata agttatggga atgccgttag tgtccataaa
     1321 cagccccatt tgaataatcg ggtttgggcg atgttccttt gaaacgccct ttgccctaaa
     1381 atcatcttcc ttgtcagact cgaaataata gttggtaaca tcataataaa ccaggctggt
     1441 atcacggccg tagatgcttt tgattcggtc attgatccat ttttgtagtt catttcccct
     1501 atcgttgaaa aagctaagtg aacgatagac atcatcaagg gaatagttag ttttttcaaa
     1561 gaactgctct ctgtcttcaa aggctttctt cttagaagaa ggtgacagca atctagagaa
     1621 aacaagtaac ttcatgatgt tattcatact ggcttcaatc tttgtagacc gttgacggct
     1681 ggtcaagaac ttatctatct caagggtgtg atagatactg ctgaaaggag cataaccgaa
     1741 attttttcgg ttgttggtgc cgataggcag ctgttcttga gaatcaaaga gaatcttaat
     1801 aggtgctttc tttaactgaa cttcattgtt catttcttcc acaacgcctt taaagtaggc
     1861 aatgggatca tcaaaatctt tttcaagctc atcaaggtat cccaaagatt ggatagttct
     1921 ggatttagtt ttcttggtat ccttatcccg aaagctttcg gcgataatta gataaacacg
     1981 atcaggattc tttttattga ttttctttaa atacataggt cacctcaact attagtagac
     2041 actcattata tcacatatct gcatgtatta acacataaat atgcataaaa aacaaaataa
     2101 aaaaagtctg aatcaattcc attcacaatg attcagactc aactatctat taacttgctg
     2161 caaaactccg gatacctact atcttgggga cccatatagg gcaacaggag actggtatat
     2221 agagaatctt gccattacaa acctggacta tatgatgacc tataagatta cagatatata
     2281 tggaaatagc tattggactc cagtaattac agattaaatt gaaatcccgg gcaaaaattg
     2341 cccgggattt ttaaatttgg gtatctggaa gagatcaata tgctatagta tatatattag
     2401 agaaaaagat tgggggaata atcatgataa gatttgaaag cgactattca gagggagccc
     2461 acaagaaaat actggacatg ctacttgaga caaacatgga gcagactcca ggctatgggg
     2521 tggaccacca ttgtgaaaat gccagaaaat acataagagc ttgctgccgg gacgacagtt
     2581 tggatgtaca ttttctagtg ggtgggactc aggcaaatac gaccgttatt gcatccatac
     2641 taaagccata ccagggagtt gtttcagcca atacaggaca tattaacgtt cacgaaacag
     2701 gtgcaattga agccactggt cacagggtta tttccatgaa tagcgaggat ggaaagctaa
     2761 cttccaacca tgttagggag gtctatgaca gccactggaa cgatgccaca cacgaacaca
     2821 tggttcagcc tggaatggtc tatatttcac atccaacgga gaacgggacc atttattcca
     2881 agaatgaact tgaagaaata agcagggtat gccaggagct taacctgccc ctctttatgg
     2941 atggggccag aatgggctat ggactaatgg cagagggcaa tgaccttagc ttgtctgata
     3001 ttgcgaggct atgtgatgta ttttacattg gaggcacaaa ggtaggtgcc cttttcggag
     3061 aggctgttgt aatcagaagc gataagttga agaaggactt caggtacaat ataaagcagc
     3121 gtggcggtat gcttgctaag ggcaggcttt tgggaattca atttgaagcc ctttttgagg
     3181 atggactgta ttttgagata tcaaaaaatg caataaagac tgcaatgatg atccgggatg
     3241 cattcaggga caagggaata gagtttttgt acgattctcc aacaaaccag cagtttccaa
     3301 tcctgaccca ccagcagatt gaaaagctgt cgcaggacta ttccttccag cattgggcca
     3361 gggttgacca tgaaagggat gcagtaaggt tttgtacaag ctggacaaca gatgaggcca
     3421 atgtaataaa gctagtccag gatatcaaaa aactttaggg acaatggccg gcaatacatg
     3481 gaaaagacct aggaaagcat agaaaaaaga cattcaaggg aaagagggag atttttggaa
     3541 ccgtttttgg gacctgtaaa ttctcccttt attgtgttat aattactgat gtatgtttaa
     3601 tggaaaacca attaaaaagg agtaggtata tgagaaaaat tggatttgac cctcagaagt
     3661 acatagaaga gcagtcgaag tatatacttg agagagtgaa caactttgac aagctatatt
     3721 tggagtttgg tggaaagctt atcggggatc atcacgctca aagggtcctt ccaggctttg
     3781 atgccgattc caagataaag ctccttcaca agctgaagga taaggcagag attataatat
     3841 gcgtctatgc aggagacatt gaaagaaaca agataagggg agactatggg atttcctatg
     3901 acatggatgt cctaaagcag atagacgagt taagggaata ccagctcaag gtaaacagcg
     3961 tagttattac cagatacaat ggacagccca cggcaacaca gtttattaca aagctggaga
     4021 gaagagacat aaaggtttat acccatacag ccatacctgg atatccttcg gatgtggata
     4081 tgattgtaag tgaagaaggc tatgggagaa atccctacat tgagacgacc atgcccatcg
     4141 tggttgtcac cgccccggga ccgggaagcg gaaagctggc aacatgcctg aatcagctct
     4201 atcacgagca taaaatggac aaggttgcgg gctattcaaa gtttgagaca ttccccgttt
     4261 ggaatgttcc actgaagcac cccctaaaca tagcatatga agctgcaaca gtggacctca
     4321 aggatgtaaa catgatggac tattttcatt ttgaaagata caatgaggtt gcagtcaact
     4381 acaacaggga ccttgaaatg ttcccggtta tcaagagaat catagagaga ataaccggca
     4441 aggaatcaat ttaccactca cccactgaca tgggagtaaa taggataggt tttgggatag
     4501 tggatgacca ggttgtaagg gatgcatcca agcaggaaat aatcaggaga agctttgcca
     4561 cagaatgtga ctacaaaaag ggccaggcag atgaggaaac ccttaatagg atgagaataa
     4621 taatggagga gcttgagctt aagcgggagg acagggctgc agttggacct gcaagggaat
     4681 actcccagag cttgaaggaa aaggaaggca agaatgatac gcctcctgta atagcaatag
     4741 agctaaatga tggaagaata atcactggta ggggatcaca gcttctggat gctacatcag
     4801 ctgcaatctt aaattcaatc aaaacactgg cgaatattgg ggatgaaatt ctacttcttt
     4861 ctcccttgat aatagataca atacagaagc tcaagatgga gagtcttatg ggcaaagtag
     4921 gaacacttga tgccaatgaa gccctgatag ccctaagcat cggagcggta acaaatccaa
     4981 cagcacaatt ggcaatggaa aagctgcagg acctaaaagg agcacaggca cactcaacag
     5041 tgatgctaaa tgataatgat gaacagatac taagaagact gggtgtggat gtaacctgcg
     5101 atccagtata tccaagcgac aacctgttct acgtatagac ttttcagcag gcaaattgtc
     5161 tgctgatttt ttctcaacaa atatacccgt acacggtata atcataaggc gtgactaaaa
     5221 aatttcaaga ggtgtaaaat gaattggtta ctttatttga ttacaatatt atttttggca
     5281 tactcatatg ttaaagataa aggaaagaca aaaaaatcct tgtccaaggc ctggaaggca
     5341 tttgaaaaca tcctcccgga atttctggtg gtaataataa tggtaggcct tatgatggca
     5401 gtacttgatc ctgaactgat agtcaaggtc attggtgagg attcaggatg gcttggagta
     5461 gttcttgcat ccattgtagg gtcggttaca cttatcccgg gttttgttgc attccccact
     5521 gcagacatcc tgcttcaggg aggtgcaggc tacatgcaga taggggcatt cgtttccacc
     5581 cttatgatgg ttgggatagt gaccattcca gttgaagtca aatactttgg tggaaagata
     5641 acagcttata gaaacgccct ggcctttgta ttctcatttt ttgtggccta tgccatcagt
     5701 ctggtggtga acctggtatg attaagacaa taatgagata cagatgggtt atcatatcca
     5761 tgatagttat gggaatagta aatatagctg acaggggtct tggttcaagg gctgttggta
     5821 cgactatgat gaccctaaag gaaatgctgt ttataatccc tcccatattt gttcttctgg
     5881 gacttctgga tacctgggtt ccaaaggata caatggtcag gtacatggga gaggattcag
     5941 gtcttaaggg agtgatattg gccttcttca tcggttctgc cgccgcaggg cccttgtatg
     6001 gagcattccc agttgctgca attttcatga gaaaaggtgt gaaattcagc aatatactta
     6061 tcttcatagg agcctggtcc acaacaaaaa ttcccatgtt ccttttcgag ctgtcagcct
     6121 tgggggcgtc ctttgcaatt acaaggctcc tcgtaaacat accagggata ataatcatcg
     6181 cctatcttct caattcactt gtctcaaggg aggaagttga gaccatgtat cttaatgctg
     6241 agagactgag cgactaaatc aatgcatact tgatgttgat aatattcaca atcagtatta
     6301 taatcatatt attcggggtg tagcgcagtt tggtagcgca catgcttcgg gagcatgggg
     6361 ccggaggttc aaatcctctc actccgacca gaaaagaatc tgccgtctat tcggcagatt
     6421 cttttatata gtccctaact cttcccattt cttcgaaaat gtacctctct tttcggtaat
     6481 aatagtatat atgattttta gcatccagga gcttggaatt tttaagaatg aattccttgt
     6541 ctccacccag cactgcaaga agaactcgtt ccatatgcct cctgaatatc tgaagccttt
     6601 catcagcact gtatttgctc acttcaca
//
LOCUS       DASRQF010000024         8079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1349247, whole genome shotgun sequence.
ACCESSION   DASRQF010000024 DASRQF010000000
VERSION     DASRQF010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 8079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8079
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349247"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1453
                     /locus_tag="VFD79_00650"
     CDS             <1..1453
                     /locus_tag="VFD79_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZH92583.1"
                     /translation="RYFFVQDEGIVQPQSATVILDYRTGHIKAIIGGRDVDGTRILNR
                     ATDSARQPGSAIKPVAVYWPALDSGMTAATAIDDIPFYDGNGTLWPKNWYNGYRGLHT
                     LRRSVEQSVNVNSVKTVQNIGVPTSVAYLEKMGIIDSEHPERDNFVTSIENRVTNDEN
                     LSALGLGGMTNGLSPLELTAAYGAIANNGVYVEPIAFSRILDRNGNVLLDNTPGETIV
                     TSPQVAYIMGDILRTTVSSGIAGRARMSNMAVAGKTGTTQNQADIWFVGYTPYYVSGT
                     WIGNDSPRVTLSRNSSTAAQMWQYINNTIHEGLEGKTSFERPDGIVSASICSQSGLLA
                     TPLCSNDPRGVVRTEIFANGTVPKAYCDMHVEVKVDVTNGKIANEYCPDEDVATRVFI
                     QREPLYDPSANNGITPSDYNYQVPTQVCDDHDENTVIENPVLDWLNEWFNNNNGEAPP
                     ENMPPATEPGNSGNGNGNGNATPPGQGDDSNNN"
     gene            complement(1513..2355)
                     /locus_tag="VFD79_00655"
     CDS             complement(1513..2355)
                     /locus_tag="VFD79_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HZH92584.1"
                     /translation="MTEILKGNVVAASIKEDMATRIAILKEKGIVPTLGIIRLGDNPG
                     DVSYEKSIIKACEKMDIQAKVYERETTMTTEDLVALMEDLNRDSEISGILLFRPLPKH
                     IDEEVIRSSISPDKDVDCMHPLNLEKIFEGNMTGFAPCTPKAAMEILNFNDIQLEGSK
                     VAVINRSMVVGKPLVMMLLEKNATVTICHSRTKNLSEVTRDADVVVTALGKPKFFGKE
                     FFNPDSIVIDVGVGMTEDGKLSGDVDYESVSEEVSMITPVPGGVGSVTTTILLNQVVL
                     AAEK"
     gene            complement(2440..3480)
                     /locus_tag="VFD79_00660"
     CDS             complement(2440..3480)
                     /locus_tag="VFD79_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HZH92585.1"
                     /translation="MHNRKHKLAFKILALCMLMGVGFKDTGVFVKSFALMNDQATEVT
                     FDKEERVTVAKEGSDSYQVLKDGTYYSIPKNVMIRTTRSSDRYTVEEATVMYKEADSN
                     SQVVKEIQPGEELVMASYDETFGYFITAESFHIGHVRMDQTTRKTTENISYGMSNITR
                     VLTNGDNKLVLVKGEAVSVKNFQNGQYEIVDENMISYHVEETAVTLFRTMEEVSRAAE
                     RGSSASIARILDAAHREIGKPYVYAAAGPNAYDCSGFTYYIFKNELGITLPRTSYSQG
                     SAGVKVEKSQLVPGDLVFFNTTGRGISHVGLYIGDGNMIHASSGKRQVRIDNINSGYY
                     SQRYVTGRRVIN"
     gene            complement(3680..4014)
                     /gene="rnpB"
                     /locus_tag="VFD79_00665"
     ncRNA           complement(3680..4014)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFD79_00665"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(4051..4776)
                     /locus_tag="VFD79_00670"
     CDS             complement(4051..4776)
                     /locus_tag="VFD79_00670"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HZH92586.1"
                     /translation="MLDLAGLWELEKHYDNLSTMRNQMKLLQESTELRRMEREIRVLE
                     ERFEDLLVKRKDLKLHLRKLEQEVDVHKDKYKSAEVNLYNGSVKDLKQLDHLEREKEY
                     HLSLVDSLEDEIIDTMGISEETEAGISEGKSLLSQKRKDLVDKQKLFDKANSDLSDRI
                     ELEEKTIGDLESDLDEKLLSIYHDIRKRKGTGIAQVRNEVCKGCNMHLPEKIVEKAKQ
                     GKSVATCESCGRILYHEMDMDNI"
     gene            complement(4792..5892)
                     /locus_tag="VFD79_00675"
     CDS             complement(4792..5892)
                     /locus_tag="VFD79_00675"
                     /inference="COORDINATES: protein motif:HMM:NF013909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HZH92587.1"
                     /translation="MKASEIISKIEEWTPRNLVDSWDNTGLQVGRPNQTVNGILIAMD
                     VTDGLVQKAIETNCNMIITHHPFLFRPLESLVFNDYKGNLIRNIILNDIVIYNAHTNL
                     DLADGGINDRLAQLFGMTSTEILSGSTSEDLVKVAVYVPKTHEGKILQALSDSGAGSI
                     GEYGDCSFSLEGTGRFRPMEGSNPYLGSTGVLESVDESRIETIVRKENLQNTLDRIRA
                     SHPYEEVALDIYPLLNKGKSHGYGRVGNIEGCSLSQLAEITKQVLQLERVRVYGTSRG
                     KIERIALCGGAGADFIRDASMKGAQVYITGDIKYHDAQLAYEEGIVLIDGTHYGTERI
                     ILPVLEKKLADYSGGKIKIEVYEDNSFNFECY"
     gene            complement(5889..6575)
                     /locus_tag="VFD79_00680"
     CDS             complement(5889..6575)
                     /locus_tag="VFD79_00680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZH92588.1"
                     /translation="MNLSPRLKKIADFVPINSIVADIGTDHGYVPKYLIDKGISKLVI
                     ATDISRGSLQKTIDYIREENLEDQIEARLGDGLEPILPFQADTGVIAGMGGLLIVEIL
                     EKSMAVAKSLNRLILQPMVGSVELRRFLHGAGFQIIDEDLVKEGDKYYEIIVAKVGLQ
                     KFANEFDYEISPVLMKKGNPLTKEMVETRLSIHEKIMDTLRDSPGEKSKARLVELDKI
                     VRDYREVLER"
     gene            complement(6617..7789)
                     /gene="rpoD"
                     /locus_tag="VFD79_00685"
     CDS             complement(6617..7789)
                     /gene="rpoD"
                     /locus_tag="VFD79_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HZH92589.1"
                     /translation="MKDTTKNPEKNFTNKDLAEKKLLDKGKKNGLLTYKEIMDTLEAV
                     DLDPEEIDEIYQRIEDSDIDIMGEKDEEILLADDDDDEDSDEDIDKVVDEELDSDDDE
                     DDVKEDLTVPKGISVDDPVRMYLKEIGKIPLLTSEEEIDLAMRMEEGEELAKKKLAEA
                     NLRLVVSIAKRYVGRGMLFLDLIQEGNLGLMKAVEKFEYRKGFKFSTYATWWIRQAIT
                     RAIADQARTIRIPVHMVETINKLVRVQRQLVQELGRDPSPEEIGQEMNLDVERVREIM
                     KIAQEPVSLETPIGEEEDSHLGDFIPDEDVLAPADAATFTMLREQLIDVLDSLTPREQ
                     KVLKLRFGLDDGRARTLEEVGKEFEVTRERIRQIEAKALRKLRHPSRSKKLKDFLE"
     gene            complement(7786..>8079)
                     /locus_tag="VFD79_00690"
     CDS             complement(7786..>8079)
                     /locus_tag="VFD79_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92590.1"
                     /translation="KEALREELSQDQESGEKFNGILNHQPRFQASNWERVIEDLIRTI
                     RHNKLISEKSLILEEMGKLEKSGEDQERFLELLGNIDELNRKINSLVSREEIN"
BASE COUNT         1993 a         2025 c         1518 g         2543 t
ORIGIN      
        1 caggtacttc ttcgttcagg atgaaggaat tgtacaaccg cagtcagcta cagttattct
       61 ggattacagg acgggacata tcaaggccat aatcggtggt agggatgttg acggaacaag
      121 aatcctcaac agggccacag attcagccag acagccggga tctgcaatca agccggtggc
      181 ggtctattgg ccagcccttg atagtggaat gacagctgct acagcaatag atgacatacc
      241 attttatgat ggtaacggaa ccctttggcc aaagaactgg tacaacggct acaggggcct
      301 ccatacccta agaaggtctg ttgagcagtc cgtaaatgta aattcagtaa aaacagttca
      361 gaatataggt gttccaacct ctgtggctta tttggaaaaa atggggataa tagacagcga
      421 acacccagaa cgtgataact ttgttacaag cattgaaaac cgtgtcacca acgatgaaaa
      481 cctatcggca ttgggccttg gaggaatgac aaatggatta agcccgcttg aattgactgc
      541 ggcttacgga gccattgcaa ataatggagt atatgttgag ccgattgcat tctccagaat
      601 cctggatagg aacggaaatg tgctacttga taacacacca ggcgaaacaa ttgttacatc
      661 ccctcaggta gcatatataa tgggagacat attgagaaca acagtatcaa gtggtatagc
      721 aggcagggcg agaatgtcca atatggcagt tgctggaaag acgggtacaa cacagaatca
      781 ggctgatatc tggtttgtcg ggtatacacc ctactatgtg tcaggtacct ggataggaaa
      841 cgactctccg agggtcaccc tttcgagaaa cagtagtacg gctgcccaga tgtggcagta
      901 catcaataac acaatccacg aaggcttgga gggtaaaacc agctttgaaa gacctgatgg
      961 catcgtttcg gccagcattt gttcacagtc aggattactg gcgacacccc tttgctcaaa
     1021 tgatcccaga ggtgttgtaa ggactgagat atttgcaaac ggaactgttc caaaggctta
     1081 ttgcgatatg catgtggaag tgaaggttga tgttaccaac ggcaagattg caaacgagta
     1141 ctgtcctgat gaggatgtag cgacaagagt atttatccag agagagcctc tttatgatcc
     1201 atctgcaaac aacggaatca cgccaagcga ttacaactac caggttccaa cacaagtctg
     1261 tgatgaccat gatgagaata cagtaatcga gaacccagtt ttggattggc taaatgagtg
     1321 gttcaacaac aataacgggg aagcgcctcc tgagaacatg ccgcctgcaa ccgaacctgg
     1381 aaacagcgga aatggaaacg ggaatggaaa tgcgacacca cctggacaag gtgatgacag
     1441 taacaataac tgatctagat tctacaaaag gcatgaaatc aattgatttc atgccttttc
     1501 ttatgagttc ttctacttct ctgctgccaa tacaacctgg tttaaaagga tagtggtggt
     1561 cacactacca acccctccag gtactggagt tatcattgaa acctcttcag aaacactttc
     1621 atagtcaacg tctccactaa gttttccatc ctcggtcatt ccaacaccaa catcaattac
     1681 aatggagtct ggattgaaaa actctttccc aaagaacttt ggctttccga gggccgtcac
     1741 tacaacatca gcatcccttg taacctctga aagattcttg gtccttgaat gacagattgt
     1801 aacggttgca tttttttcaa gaagcatcat tacaagaggc tttccaacca ccatggacct
     1861 gttaatgact gcaaccttgc ttccctcaag ctgtatgtca ttgaaattga ggatttccat
     1921 ggctgcctta ggtgtacagg gagcaaatcc ggtcatattt ccctcgaata tcttttcaag
     1981 attaagggga tgcatgcaat ccacatcctt gtcagggctg attgagcttc ttatcacctc
     2041 ttcatcaata tgctttggaa ggggtctgaa aagaagtatt cctgatatct ccgaatccct
     2101 gttaaggtct tccatcaagg ctaccaggtc ttctgttgtc atggttgttt ctctctcata
     2161 tacctttgcc tgtatatcca tcttctcaca ggccttgatt atgctctttt cgtaggaaac
     2221 atccccagga ttatcaccaa gccttattat tcccagagtt gggactatgc ctttttcctt
     2281 caatattgct attctcgttg ccatgtcttc cttgatgctg gctgcaacta catttccttt
     2341 caatatttca gtcatgccat acctccttgt gaatttgtta cattaattat aacatagaaa
     2401 aaaaacaaaa gcacccatag ggtgcttaaa gtgcatattc taattgatga ctcttcttcc
     2461 agttacatac ctttggctgt agtagcccga attgatattg tcaattctaa cctgtctctt
     2521 gcctgaggat gcatggatca tgtttccatc gccaatatac aagccaacat gggatattcc
     2581 tctgcctgtt gtgttgaaga ataccaggtc acctggtaca agctgtgact tttcaacctt
     2641 aacccctgca gatccctgtg aatatgatgt tctgggcagg gttattccaa gttcattctt
     2701 gaaaatatag taggtgaatc ctgagcaatc ataagcattg ggtccagcag cagcatatac
     2761 gtaaggcttg ccgatttccc tgtgtgccgc atccagtatg cgagctatgg atgcacttga
     2821 tcctctttca gctgcccttg atacctcttc catggttctg aacagtgtca ctgcagtctc
     2881 ctccacatgg tagcttatca tgttctcgtc gactatttca tattgcccat tctggaaatt
     2941 cttcacagaa acagcttcgc ccttaaccag gaccagttta ttgtccccat tggtcaatac
     3001 cctcgtaatg tttgacattc cgtaggatat gttttcagtt gtttttcttg ttgtctgatc
     3061 catgcgaaca tgaccgatat gaaagctttc cgctgttatg aagtagccga aggtttcatc
     3121 gtagcttgcc atcacaagct cttccccagg ctgtatttcc ttcacaacct gtgaatttga
     3181 gtctgcttcc ttatacatta cagtggcctc ttcaacagta tatctgtcag agcttcttgt
     3241 ggtcctaatc attacattct ttggtatgct gtagtatgta ccatccttca acacctggta
     3301 ggaatcgctt ccctcctttg caactgtgac cctttcttcc ttgtcaaaag taacttcagt
     3361 ggcctgatca ttcatcagtg caaagctttt tacaaataca cctgtgtctt taaatccaac
     3421 tcccattagc atacaaagcg ccaatatctt gaaagccagt ttgtgttttc tattgtgcaa
     3481 tatgaacact cccttatctt cgattagtct cataaaggta ttataataca aggaaggtcc
     3541 tttttcaatg aattatttac aaaacggtta caaagttaaa tcatcattgc tttaaacgtt
     3601 gtattttcaa caatcatgag aatatgtctt tctcgttaca tataagagaa aacctccggg
     3661 tatctcccgg aggttttcac gtttgagtaa gtctataagc cgtgttctgt ttctatggtc
     3721 atctatctag gtctgcagtt acctgcagac tcatgcgacc tacctttgga gcggcaacga
     3781 gcaactgcct ttttgtgctc ccttttggtc ttgctccgga tggggtttac agagccacct
     3841 agtcgcctag atgctggtga gctcttacct cacctttcca tccttaccat ttctggcggt
     3901 ctatttctgt tgcactatcc ttagggttgc ccccactgga cgttatccag catcctgctc
     3961 tacggagcac ggactttcct catgtcgtag acatgcgacc atacaactta ctctaagtat
     4021 attatatcac agatgccttt tctttaaaac ctaaatgttg tccatgtcca tctcatggta
     4081 gagaatcctt ccacagctct cacatgtagc cacacttttc ccctgctttg ccttttctac
     4141 aattttttcc ggtaggtgca tgttgcaacc cttgcaaact tcatttctca cctgggcaat
     4201 accagtgccc tttctttttc tgatgtcatg gtagatggaa agtagtttct catcaaggtc
     4261 actttcaaga tccccaatgg ttttctcttc caattcgatc ctgtcactta aatctgaatt
     4321 agccttatca aagagcttct gcttatccac aaggtccttc cttttctgtg acagaagact
     4381 tttgccttca cttatgcctg cttcggtctc ctcgcttatg cccattgtgt caattatttc
     4441 atcctccagg ctatcaacca ggcttaggtg atactccttt tccctttcta ggtggtccag
     4501 ttgttttagg tcctttactg agccattgta gagattcact tctgcagatt tgtatttgtc
     4561 cttgtggaca tcaacttcct gctcaagctt tctaaggtga agcttcaggt ctttcctctt
     4621 gaccaacagg tcttcaaacc tctcttccaa aactctgatc tccctttcca tcctcctcaa
     4681 ctcggtactc tcctgtaaaa gcttcatctg attcctcatc gtactaagat tgtcgtagtg
     4741 cttttcaagt tcccacaaac cagccaggtc cagcattaga agtcctccta tctagtagca
     4801 ttcaaagtta aaagaattat cctcgtaaac ttcaattttg atttttcctc cactgtagtc
     4861 tgctagcttt ttctctagaa caggaagaat tattctttcg gtgccgtaat gggtgccatc
     4921 aatcaaaaca ataccttcct cgtatgccag ctgggcgtcg tggtatttta tatctcctgt
     4981 aatatacacc tgtgcccctt tcatggatgc gtcccttatg aaatccgccc ctgctccacc
     5041 acaaagtgct atcctttcaa ttttacccct gctagtgccg tataccctaa ccctttcaag
     5101 ctggagtacc tgttttgtga tttccgccaa ctgggaaagg ctacatccct ctatgttccc
     5161 gactctaccg tagccatggg acttgccttt gttcagcaaa ggataaatgt ccaaggctac
     5221 ctcctcatat gggtggctgg cccttatcct atccagggta ttctggagat tctctttcct
     5281 gacaattgtc tcaatccttg attcatcaac tgactccaaa acacctgtgg aacccagata
     5341 ggggtttgag ccttccatgg gtctgaatcg tcctgttcct tccagtgaaa aactgcaatc
     5401 accgtactca ccaatggatc ctgctcccga atccgataat gcctgaagta tctttccttc
     5461 atgtgtctta gggacataga cagcaacctt tacaaggtct tcacttgttg aaccgctcaa
     5521 tatctcggtg ctggtcattc caaacagctg ggcaagcctg tcatttatcc caccatcagc
     5581 caggtccagg tttgtatgtg cgttgtatat gacaatgtca ttaaggatga tatttcttat
     5641 caggttgccc ttgtagtcat tgaaaaccag actttccaga ggcctgaaca aaaatgggtg
     5701 gtgggtaatt atcatgttgc agttcgtttc aattgccttt tgtacaagtc catccgttac
     5761 atccatggct atgagtattc cattaactgt ctggttgggc cttccaacct gaagacctgt
     5821 gttgtcccat gaatccacaa gatttctcgg tgtccattct tcaattttac tgattatttc
     5881 agaggctttc acctttcaag cacctccctg taatctctca ctatcttgtc cagctccaca
     5941 agtcttgcct tgcttttttc tcctggacta tcccttagtg tgtccattat tttttcatgt
     6001 atacttaatc ttgtctctac catctctttt gtcaggggat ttcctttctt cattaagact
     6061 gggcttattt cataatcaaa ttcattggca aatttctgta gtccgacttt tgcaacaata
     6121 atttcgtaat acttgtctcc ctctttaacc aggtcctcat caattatctg aaaaccagct
     6181 ccatggagaa accttctcaa ctccacagat cctaccatgg gttgaagtat cagcctgttg
     6241 aggctttttg caacagccat tgacttctct agaatttcca cgatcaacaa tcctcccatt
     6301 cctgctatca caccggtgtc agcctggaag ggaagaatgg gttcaagacc atctccgagt
     6361 ctggcctcta tctggtcttc aagattctct tcccttatat agtcaattgt cttctggaga
     6421 gatcccctgc ttatgtcagt ggcaatcaca agcttgctga tccccttatc tatgagatac
     6481 ttgggaacat agccgtgatc ggtgccaatg tcagccacaa tggaatttat tggtacaaaa
     6541 tcagcaattt tcttcagtct aggtgataga ttcataattt ctccttttca tgggaataag
     6601 atccgcaaag cgaatcttat tcaagaaagt cctttaattt tttactcctg ctggggtgac
     6661 gaagctttct caatgccttt gcctcaatct gtcttattct ctctctggtt acctcaaact
     6721 ccttgccaac ctcctcaagg gtcctggccc taccgtcatc cagtccaaat ctaagcttca
     6781 gcaccttctg ctctctggga gtcaagctgt ccaacacgtc aatcagctgt tccctaagca
     6841 ttgtgaaagt ggctgcatca gctggtgcaa gtacatcctc atctggtatg aagtctccaa
     6901 ggtggctgtc ttcctcttct ccaatgggag tttcaaggga aactggctct tgagctatct
     6961 tcataatttc tcttaccctc tcaacatcca agttcatttc ctgaccgatt tcctctgggg
     7021 atgggtcccg tccaagttcc tggacaagct gcctttgaac tctgacaagt ttatttattg
     7081 tctcgaccat gtgcacagga atcctgatgg tccttgcctg gtcagctatg gcccgtgtaa
     7141 tggcctgtct aatccaccag gtggcatagg tactaaattt aaatcccttc ctgtactcga
     7201 atttctcaac tgccttcatc aggcccagat taccttcctg aatcaggtca aggaatagca
     7261 tcccccttcc cacgtatctc ttggctatac tcaccacaag cctcagattg gcttctgcga
     7321 gctttttctt ggccagctct tccccttcct ccattctcat ggcaaggtca atctcctcct
     7381 cgctggtgag caggggaatc tttccgattt ccttcagata catcctgaca gggtcatcta
     7441 cacttattcc ttttggtacc gtaaggtctt ccttgacatc atcttcgtca tcatcactgt
     7501 ccaactcttc atccacgacc ttgtcaatat cctcatcact gtcctcatca tcgtcgtcat
     7561 cagccagcag gatctcctca tccttttcac ccatgatatc aatgtcactg tcctctattc
     7621 tctgatagat ttcatcgatt tcttccgggt ccaaatctac ggcttccaaa gtgtccatta
     7681 tttccttgta tgtcaacaag ccgttctttt tacccttgtc caataatttc ttttcggcca
     7741 ggtctttatt tgtgaaattt ttttcagggt tcttagttgt atccttcaat ttatttcctc
     7801 cctacttact aatgaattaa ttttcctgtt cagttcatct atattgccaa gcaattcaag
     7861 aaatctttcc tggtcttctc cacttttttc aagcttcccc atttcctcaa gaattaaact
     7921 cttctcactg attagcttgt tgtgtctaat tgtcctaata aggtcctcga tcaccctctc
     7981 ccagttggat gcctggaatc taggttgatg attcaaaata ccgttgaatt tttctccact
     8041 ttcctgatcc tgggatagtt cttcccttag tgcttcctt
//
LOCUS       DASRQF010000025         3752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1470304, whole genome shotgun sequence.
ACCESSION   DASRQF010000025 DASRQF010000000
VERSION     DASRQF010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470304"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="VFD79_00695"
     CDS             <1..1046
                     /locus_tag="VFD79_00695"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HZH92591.1"
                     /translation="GLGPTDDDLSKEALAHVTGKTLVSDINMEHHIKHFFAYSQREMT
                     VNNLKQAMRPEGSEFIDNPRGTAPGIYMEWKNRKIVMMPGPPREMKPMFENSVVDLIK
                     DNHIIIVKSINTSGLGESTLETQLKKLDLSYPDFSITTYAGSGSVEIKVIGRGQDKSK
                     LEKESEEIVESIRGEIGDHIYGYGISSLESTIIETLTLNGMKIAACESVTGGNVSRRL
                     TSIPGASKAFDRGLVTYSDQAKRQELGISREDLDKYGAVSREVACKMAEGLLKKSNTD
                     IVVATTGYAGPDHDIDVKTGLIYVCIMSRTNHEVYERIFSGDRDTIQERATNFALWNV
                     YKFLNKELTSIKR"
     gene            1118..2146
                     /gene="recA"
                     /locus_tag="VFD79_00700"
     CDS             1118..2146
                     /gene="recA"
                     /locus_tag="VFD79_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005956439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HZH92592.1"
                     /translation="MAENNERKKALDLALSQIEKQFGKGSIMRLGEDAKLNLDYIPTG
                     SMDLDIALGIGGVPRGRIVEIYGPESSGKTTIALHIIAEAQKKGGIAAFIDAEHALDP
                     GYAKKLGVDIENLIISQPDTGEQALEITEALVRSGAVDVAVIDSVAALVPKAEIEGEM
                     GDSHVGLQARLMSQALRKLAGAINKSNTTCIFINQLREKVGIMFGNPETTSGGRALKF
                     YASVRMDVRRIESIKQGDQILGNRTRVKVVKNKIAPPFKQAEFDIMYGKGISREGSII
                     DTGVTCGIVNKAGSWFSYNDQKLGQGRENSKEFLRENPELAMELELKIKKEFGLEIND
                     EKEVAPEK"
     gene            2195..3163
                     /locus_tag="VFD79_00705"
     CDS             2195..3163
                     /locus_tag="VFD79_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZH92593.1"
                     /translation="MRILYFTDTHIKGTNPKNRKDDYIESLEDKFREIVSIVKEKDID
                     FVIHGGDLFDRPDISISVVSRFTTILKEIDKPIYIVSGNHDIFGHNPATIDRTMLGLL
                     RSLDFIKVIPRDKPVIIEKMGIRVSLTSLPYAYDIDSTPDGYILKESPKDIDYSIHVV
                     HGMLLDRPFVKGIPYTLVDDILETAADITLSGHYHAGFKTIEKNGKYFVNPGSLVRVT
                     NSLKEIERMPQVAIIQMDSQIKIDMLPLKSAKPGEDVLDRTEMENSTLRSERIVQFKQ
                     SIDSAMSFEKMDLNDILVEVTLSEGVSDKVKEEALRRIANSQMKGY"
     gene            3171..>3752
                     /locus_tag="VFD79_00710"
     CDS             3171..>3752
                     /locus_tag="VFD79_00710"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZH92594.1"
                     /translation="MRYIKKIILENFQSHKNTEMEFGPDLNVIVGPSDSGKSAIIRGL
                     KWVMYNEPSGDFFIREGEKECRVTLELSDGTLLQRYRTKSKNGYKLIDNSGNENIYEG
                     IGLTVPQEIIDVTGIRKIKLDTDSENAINLGEQLEGAFLLTEKASTRANAIGRLVGVD
                     IVDDALRDVLRDIRSLNQTRKQKEETLQITNDKL"
BASE COUNT         1274 a          631 c          917 g          930 t
ORIGIN      
        1 gtggactggg gccaacggat gatgaccttt ccaaggaggc cctagcccat gtaactggaa
       61 agacactagt ttctgatatc aacatggagc accacatcaa gcactttttt gcatattccc
      121 aaagggaaat gacagtgaac aatctaaagc aggccatgag accggaggga tcggagttca
      181 tagacaatcc acggggaacg gctcccggga tttatatgga gtggaaaaac agaaaaattg
      241 tcatgatgcc gggaccgcca cgggaaatga aaccaatgtt tgagaacagt gtggttgact
      301 taatcaaaga taatcatatc ataattgtta agtcaataaa tacatctggt ttgggtgaat
      361 caacccttga aacacaattg aaaaaactgg acctgtcata ccctgatttt tcaataacca
      421 cctatgcagg gtctggtagt gttgaaataa aagtaattgg taggggacag gacaagtcta
      481 aacttgaaaa agaatctgag gaaatcgttg aatcaataag aggtgaaata ggggaccaca
      541 tctatggata tggaatttct tcactggaaa gtacaattat tgaaacactt acactgaatg
      601 gaatgaaaat tgcggcatgt gaatctgtta ccggaggaaa tgtttccagg agattaacaa
      661 gtatacctgg ggcatccaaa gcttttgaca gagggcttgt aacctacagt gaccaggcca
      721 agagacagga gcttggcatt tccagggaag atttggacaa gtacggagca gtcagtaggg
      781 aggttgcctg taagatggct gagggtttgc taaagaagag caacacagat atagtggttg
      841 caactactgg ttatgccggc ccagatcatg atattgatgt taaaacaggt ctgatatatg
      901 tttgtataat gtctagaacc aatcacgaag tatatgaaag gatattttct ggagacagag
      961 atactatcca ggaaagggcc acaaactttg ccctttggaa tgtatataaa tttttaaaca
     1021 aagagttgac ttcaattaag cggtagtgta acatatatat aaaagaacta atgttcgaat
     1081 atgcgaaagg ggtgaatcca gtttaactgg agatattatg gcggagaata acgaaagaaa
     1141 gaaagcatta gacctggcac taagtcagat agaaaaacaa tttggcaagg gatcgattat
     1201 gagacttgga gaggatgcta aattgaacct ggactacata ccaaccgggt caatggatct
     1261 tgacatcgct ctcggtatcg gtggggttcc aagaggaaga attgtagaga tttatgggcc
     1321 agagtcttcc ggtaagacca ccatagccct tcacatcata gcagaagccc aaaaaaaggg
     1381 gggaatagcg gcatttattg atgcagaaca cgcactggac cctggatatg caaagaagct
     1441 gggcgtcgat atagaaaacc ttataatttc acagccggat actggtgagc aagccctgga
     1501 aataacagag gctcttgtca gaagtggagc tgttgatgtt gcagttattg actctgttgc
     1561 tgcccttgtt cccaaggcag aaattgaagg tgaaatggga gacagtcacg tgggtttgca
     1621 ggcaagattg atgtcacagg cccttaggaa gttggcggga gcaatcaaca agtccaacac
     1681 aacatgtatc tttatcaacc agctacgaga aaaggttggt ataatgtttg gaaatccgga
     1741 aacaacatct ggaggtaggg ctttaaagtt ttatgccagt gttagaatgg acgtaagacg
     1801 aattgaatcc atcaaacagg gggaccagat acttggaaac aggaccaggg tcaaggttgt
     1861 aaagaataag attgcaccac cctttaagca ggctgaattt gatataatgt atgggaaggg
     1921 tatctccaga gaaggtagca taatcgacac tggtgttacc tgtggcattg taaacaaggc
     1981 cggatcctgg tttagctaca atgaccagaa gcttggtcag ggaagggaaa actccaagga
     2041 attccttagg gagaatcctg aattggcaat ggagctcgag ttgaagataa aaaaggaatt
     2101 tggtcttgaa ataaatgatg aaaaagaagt agctcctgag aaatagttaa atcaagcctc
     2161 ctgaataggg ggcttgaagt atatggaggt ttttatgcga atactttatt tcacagatac
     2221 acacattaag ggaacaaacc ccaagaacag aaaagatgac tatattgaaa gtcttgaaga
     2281 taagttcagg gaaattgtct caattgttaa ggaaaaggat atcgattttg taatacatgg
     2341 gggtgacctc tttgacaggc ccgatatctc catatctgtg gtcagtagat ttacaacaat
     2401 acttaaagag attgataagc caatttatat agttagcggg aaccatgata tttttgggca
     2461 caatcctgca accattgaca gaacaatgct aggtctcttg aggagtctgg acttcataaa
     2521 ggttataccc agggataaac ctgttattat tgagaaaatg ggaattaggg ttagtcttac
     2581 tagtctgccc tatgcttatg acatagattc cacacctgat gggtatatac ttaaggaaag
     2641 cccaaaagat attgactatt caatacatgt ggtacacggt atgctattgg acagaccttt
     2701 tgtaaagggt attccctata ccctagtgga tgatatcctg gaaacagccg cagatataac
     2761 attatctgga cactatcatg ctggtttcaa gaccattgaa aaaaatggga agtactttgt
     2821 taatccaggc agtcttgtga gagtcacaaa cagtttaaag gaaattgaaa ggatgcccca
     2881 ggttgccata atccaaatgg actcacaaat caaaatcgac atgctccctt taaaatccgc
     2941 aaaacctggt gaagatgtgc tggatcgcac ggaaatggag aattctacac tacgaagtga
     3001 acggatagta caattcaaac aatcaattga ctctgccatg agttttgaaa aaatggatct
     3061 aaacgatata ctggtggaag tcaccctttc agaaggagtt tctgacaagg tgaaggagga
     3121 ggctttgagg agaattgcaa attctcagat gaaaggatat taggtgtgtc atgaggtaca
     3181 ttaaaaagat aattcttgag aactttcaaa gtcacaaaaa tactgagatg gaatttggtc
     3241 cggacctgaa tgtaatagtg ggaccgtcag attcaggtaa atcggccata ataagaggtc
     3301 taaaatgggt tatgtataat gaaccttcag gagacttctt cattagggaa ggcgaaaagg
     3361 agtgcagggt gacactggaa ttaagtgatg ggacactact ccagaggtat agaacaaaaa
     3421 gcaaaaatgg gtacaagtta atcgacaaca gtggaaatga gaacatctat gaaggtatag
     3481 gcctaactgt accacaggaa ataattgatg taaccggtat tagaaaaatc aagcttgata
     3541 ctgattctga gaatgcaatc aatcttggag aacagctgga gggtgccttt ttattgacag
     3601 agaaggcatc gactagagca aatgcaattg gtagactggt aggggttgac atagttgacg
     3661 atgccttgag ggatgtttta agggatatca ggtcccttaa tcaaactcgc aaacagaagg
     3721 aggaaaccct ccagataaca aatgacaagc tt
//
LOCUS       DASRQF010000026         5518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1847527, whole genome shotgun sequence.
ACCESSION   DASRQF010000026 DASRQF010000000
VERSION     DASRQF010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5518
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1847527"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VFD79_00715"
     CDS             complement(<1..765)
                     /locus_tag="VFD79_00715"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HZH92595.1"
                     /translation="METVWIPFVVAAVIAFAATPFVIKTAPKVGAMDIPKDDRRVHKD
                     PMPLIGGVAMYLAIAVGMFVFLPKTRDFISIFVGATIILISGIKDDIGGTTPKEKLFY
                     QLLAAGIVIWGGVQVDFVTNPFADGNPILYLSWIGIPITLFWIVGITNTLNLIDGLDG
                     LAGGVALISALSFTFVALKLEMNQIAILAALVSGACLGFLPFNFNPAKIFMGDTGALF
                     LGFMLAVISIEGVMKSVATIAMLVPIIILGVPIFDTT"
     gene            complement(901..1530)
                     /gene="upp"
                     /locus_tag="VFD79_00720"
     CDS             complement(901..1530)
                     /gene="upp"
                     /locus_tag="VFD79_00720"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014471148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HZH92596.1"
                     /translation="MSKVVIMDHPLIKHKLTMLRDKNTGSKEFRELVTEISTLMAYEV
                     TRDFPLQDIEIETPITKMMSPVISGKKVGLIPILRAGLGMVDGMLSLIPAAKVGHVGL
                     YRDPETLQAVEYYCKLPQDVAERDLIVLDPMLATGVSAISAIQFLRKSGAKSIRLACL
                     IAAPEGVKAVQEAAPDVDIFLASLDEKLNDHAYIVPGLGDAGDRLFGTK"
     gene            complement(1573..2010)
                     /gene="rpiB"
                     /locus_tag="VFD79_00725"
     CDS             complement(1573..2010)
                     /gene="rpiB"
                     /locus_tag="VFD79_00725"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009431620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HZH92597.1"
                     /translation="MIIGIGSDHGGFDLKEEMISFLKENGYQVKDFGTHSKESVDYPD
                     FGRVVGEAVVAGEIDRGVVICGTGIGISISANKVKGVRAALCGDVYSARMSREHNNAN
                     VLAMGGRVLGVDLAKEILSTYLKSEFQGGRHERRVNKIAEMED"
     gene            complement(2015..2467)
                     /locus_tag="VFD79_00730"
     CDS             complement(2015..2467)
                     /locus_tag="VFD79_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HZH92598.1"
                     /translation="MKILFVCTGNTCRSPMAEGILRDEARKRNIGIEVASAGIFAVDG
                     DRASKGARLSMKSRGGLPDHRSRFLDDEVMEWADLVLVMTGAHKRNLIARYPQMEDKT
                     YLLHEFATGREIDVEDPFGGPLEVYEAVRMQLEEAVKRLLDKIDQQEV"
     gene            complement(2476..3522)
                     /locus_tag="VFD79_00735"
     CDS             complement(2476..3522)
                     /locus_tag="VFD79_00735"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015777686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZH92599.1"
                     /translation="METELIKINDLDGQGKEMERAAALIRAGEVVAFPTETVYGLGAD
                     GLSQPAIEKIFKAKGRPQDNPLILHVDSVNMLKRLVHDISEEAYKCMDRFWPGPLTIL
                     FRKSDLVPDIITAGLQTVAIRMPSHPIALDLIRRSKTPIAAPSANTSGRPSPTRASHV
                     VQDMEGKIAMIIDGGETGVGLESTVLDLTEDRPMILRPGGVTLEDIRDLLPGVIMDPG
                     LGDKEDFTPRSPGQKYRHYAPKAEMILFSGDREKVVAEILERAQAERAKGIRVGLLVT
                     EENRDSYPGENVIVMGSVKDKKTIAHGLFHAIRRLDEMEVDLILCESVEEDQIGMAIM
                     NRLLKAAGGNRINL"
     gene            complement(3573..4643)
                     /gene="prfA"
                     /locus_tag="VFD79_00740"
     CDS             complement(3573..4643)
                     /gene="prfA"
                     /locus_tag="VFD79_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002840294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HZH92600.1"
                     /translation="MFDKLNFLEDKYKELVQKTMDPDLINSDREEWQRVMKEQSDIEP
                     IVNKYREYTKTKQELEDAKAMLHEKLEDEMKEMVKEEVKELEEGLEKIQEEIRVLLIP
                     KDPNDHKNVIVEIRGGAGGDEAALFAGVLFRMFMRYAERNNWKTEILSTNEIGIGGYK
                     EVIFMIKGKGAYSRLKYESGVHRVQRVPETESSGRIHTSTVTVAVLPEAEDIDIEVNQ
                     GDLKIDVFRSSGNGGQSVNTTDSAVRMTHIPTGIVVSCQDEKSQLKNKEKALKILKSK
                     LYDLKLSEQNAELAQMKKGQVGTGDRSERIRTYNFPQGRVSDHRINLTLYKLNNFVDG
                     DLDEMIDALITSDQAEKMQEIS"
     gene            complement(4734..5285)
                     /locus_tag="VFD79_00745"
     CDS             complement(4734..5285)
                     /locus_tag="VFD79_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HZH92601.1"
                     /translation="MSLSLFTTFFKIGAFTFGGGYAMLPIIQKELVDIKKLISEEEFM
                     DAIALSQASPGSVALNTSVYVGYRIRGFRGALASTLGSVLPSFIIILLIAMYFFRYRH
                     NEFIDSIFMGIRPAIVALILSAVYQMWKNSRKTYIKLFASIATVGLVVFTSLNPIWIV
                     IAGAVSSVVYHKFYWPFQEERER"
     gene            complement(5299..>5518)
                     /locus_tag="VFD79_00750"
     CDS             complement(5299..>5518)
                     /locus_tag="VFD79_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HZH92602.1"
                     /translation="FKKSPYVNWIFKGIRPVVLGLIAAAAVSVGGNTFVDLKSVLIAI
                     LLFVLVTFRDLNPIFGIVLAGALGVLFF"
BASE COUNT         1337 a         1505 c         1060 g         1616 t
ORIGIN      
        1 tgtggtgtcg aaaattggga ctcccagaat gattattggc accagcattg caatggtagc
       61 aacgcttttc atgacccctt ctatggaaat gaccgccagc ataaagccca ggaagagtgc
      121 tcctgtatcc cccatgaaaa tctttgcagg gttgaagtta aagggtagaa atcccaggca
      181 agcccctgac acaagggctg caagtattgc tatctgattc atttcaagct tgagggctac
      241 aaatgtgaag gaaagggccg aaatcaaggc aactccacct gccagcccgt ccagtccgtc
      301 tatgaggttt aatgtgtttg tgatccccac tatccaaaac aatgttatcg gtatgccaat
      361 ccagcttaaa tagaggattg gattcccatc tgcaaagggg ttggttacaa aatccacctg
      421 aactccaccc cagatgacta tacctgctgc cagaagctgg tagaaaagct tctcctttgg
      481 tgtggttcca cctatatcat ccttgatccc tgaaataagg attatggttg cccctacaaa
      541 tatagagatg aaatccctgg tctttggcag aaacacaaac atcccaacgg cgattgcaag
      601 gtacatcgca accccaccta ttaaaggcat tgggtcctta tgcactcttc tatcatcctt
      661 gggtatatcc attgcaccaa ctttgggggc cgttttaatt acaaagggtg tggccgcaaa
      721 tgctattaca gctgcgacca caaaaggtat ccatactgtc tccatagtgt aattctccta
      781 atctggtata atctaaattc tatctgactg ttataaataa gtcaaacaaa taagggtgac
      841 aattgtatta caatagggtt tcaaaaatca gtaaaaggca gcctaaactg cctttaatct
      901 ctacttggta ccaaatagcc tgtctccagc gtctccaaga ccaggtacta tatatgcgtg
      961 gtcattaagc ttctcatcca gtgatgccag gaaaatgtcc acatccggtg cagcttcctg
     1021 aacagccttg actccctcag gtgcagcgat tagacatgcc agcctgatgc tttttgctcc
     1081 acttttcctt aagaattgaa ttgcagaaat tgccgatact cccgttgcaa gcattgggtc
     1141 aagtacaatc aggtctcttt cagcaacatc ctgtggcagc ttgcagtagt attcaactgc
     1201 ctggagtgtt tccggatccc tgtaaagtcc aacgtgccca accttggcag ccggaataag
     1261 gcttagcatt ccatccacca ttccaagacc tgccctcagg atgggtatca gaccaacctt
     1321 ctttcctgat attacaggtg acatcatctt agtgatgggt gtttcaatct ctatgtcctg
     1381 caggggaaaa tccctggtaa cctcatatgc cataagggtt gaaatttcag taaccagctc
     1441 cctgaattcc tttgaccctg tgttcttgtc ccttaacatt gtcagcttat gctttattaa
     1501 tgggtgatcc attattacaa ctttcgacat aacatcgctc ccttcatttt catcctaata
     1561 attataccac aatcaatctt ccatttcagc aattttattg actcttcttt catgtcttcc
     1621 accctggaat tcacttttca ggtaggtgga aagtatttcc ttggccaggt ccactccgag
     1681 aacccttccc cccatggcca gtacattggc attgttgtgc tccctgctca tccttgctga
     1741 atatacatct ccacaaaggg cagcccttac tcccttgacc ttgtttgcgg atatggatat
     1801 tccgattccc gtaccgcata tcacaacacc tctgtctatt tcaccggcca cgacagcctc
     1861 cccaacaacc cttccaaagt cagggtagtc cactgattcc ttgctgtgtg tgccaaagtc
     1921 cttcacctgg tacccatttt ccttaagaaa gcttatcatt tcctccttaa ggtcaaatcc
     1981 tccatggtca cttccaatgc cgattatcat atccttatac ctcctgttga tctattttat
     2041 caagtagtct tttcactgcc tcttccagct gcattcttac ggcttcgtaa acctccaagg
     2101 gtcctccaaa tggatcctcc acgtcaattt ccctgcccgt ggcgaactca tgtagaagat
     2161 aggtcttatc ctccatttgc ggataccttg caatgaggtt ccttttatgg gctccggtca
     2221 ttacaaggac caggtctgcc cattccatga cctcatcatc caggaacctg gacctgtggt
     2281 cgggaagtcc acctctgctt ttcatggaca accttgcccc cttgctggcc cggtctccat
     2341 ccactgcaaa aatcccagct gaggccacct ctattccaat gtttctcttc ctggcctcat
     2401 cccttaggat cccttcggcc atgggacttc tacaggtatt tccagtacat acgaataata
     2461 tcttcatggt tcctcctaaa ggtttatcct atttccaccg gcagccttaa gcaaccggtt
     2521 cataattgcc atgcctatct ggtcctcttc caccgactcg cacaggatca ggtcaacctc
     2581 catctcatcc agccttctga ttgcatgaaa aaggccgtgg gcaatggtct tcttgtcctt
     2641 gacagagccc atcactataa cattttctcc ggggtaggaa tccctgttct cctcagttac
     2701 caataggcct acccttatcc ccttggccct ttcagcctgg gctctctcga ggatttcagc
     2761 caccactttt tccctgtctc cagaaaagag tatcatctca gcctttgggg cgtaatgcct
     2821 gtacttttgt cctggcgacc tgggtgtgaa gtcctccttg tccccaagtc ccggatccat
     2881 tatcacccct ggcaagaggt ccctaatatc ttcaagggtc accccaccag gcctaagtat
     2941 cattgggcgg tcttctgtaa ggtccagaac cgtagactca aggcccactc ctgtttctcc
     3001 cccgtcgata atcattgcaa tcttaccctc catatcctgg accacatggg aggcccttgt
     3061 gggacttggc cttcctgatg tattggcaga tggtgcagct attggggttt tagaccttct
     3121 gatcaggtcc agggcaatcg gatgactggg catccttatg gcaacagtct ggagtcctgc
     3181 agtgataata tccggaacaa ggtctgattt tctaaaaaga atggtcaagg gtcccggcca
     3241 gaacctgtcc atgcacttat atgcttcttc ggaaatatcg tggacaagcc ttttaagcat
     3301 gttcacagaa tccacatgga gaatcagggg attgtcctgg ggtctgccct tggccttgaa
     3361 tatcttctcg attgcaggct gtgatagtcc gtcagccccc aggccataga ctgtctctgt
     3421 gggaaaggca accacctctc cagcccttat aagggctgcc gccctttcca tctctttgcc
     3481 ttgcccatcc aagtcattta tttttataag ttctgtctcc atttcccaac ctcgtttcca
     3541 taaactcaaa aacaaaagag ggtctcccct ctttagctga tttcctgcat tttttccgcc
     3601 tggtccgatg tgataagcgc atctatcatt tcatccagat ctccatctac aaagttgttc
     3661 agcttataca gagtcaggtt tattctgtgg tcactgaccc ttccctgtgg gaagttgtat
     3721 gtcctgatac gctccgacct gtcaccagtt cctacctggc ccttcttcat ttgagccagc
     3781 tccgcattct gctcagaaag cttaaggtca tacagcttcg acttgaggat tttcaatgcc
     3841 ttctccttgt tcttcagctg actcttctca tcctgacagg acacaactat tcccgttggt
     3901 atatgagtca tcctcactgc agagtctgtg gtgtttacag actgtccacc gtttcctgat
     3961 gaacggaata catctatctt caagtcaccc tgatttactt ctatatcgat atcctcagcc
     4021 tctggaagta ctgcaaccgt aactgttgaa gtgtgaatcc ttccacttga ctcggtctct
     4081 ggaacccttt gcaccctgtg tacccctgac tcgtacttaa gccttgagta ggctcccttg
     4141 cccttgatca taaagattac ttccttgtaa cctccaatgc ctatttcgtt ggtgctcagg
     4201 atttctgtct tccagttgtt tctctctgca tatctcataa acatcctaaa gagaacgcct
     4261 gcaaaaaggg cagcctcatc accgccggct ccacctctga tttcaacgat tacgttctta
     4321 tggtcgttgg gatcctttgg tatcagaaga accctgattt cctcctggat tttttccagg
     4381 ccctcctcca actcctttac ttcctccttg accatttcct tcatttcatc ctcaagcttt
     4441 tcatggagca ttgcctttgc atcctcaagc tcctgctttg ttttggtgta ctccctgtac
     4501 ttgttaacga taggctcaat atcagattgt tccttcatta ccctttgcca ttcctccctg
     4561 tcactgttga tcaggtctgg gtccatggtc ttttggacaa gttccttgta tttatcctct
     4621 aaaaaattca gtttgtcaaa cactgttaaa aacctctctt cattttattc gttcacatat
     4681 ctatccatac tattattata taattaattt atccatatta caagacagta cagctagcgc
     4741 tccctttcct cctggaatgg ccagtagaac ttgtggtaga ccactgagga taccgcccct
     4801 gcaattacta tccagatggg gttaaggctg gtgaagacca ccagaccaac cgttgcaatg
     4861 gatgcaaaca gcttgatgta ggttttcctt gaattcttcc acatctgata gacagctgaa
     4921 agtataaggg ccacaatcgc tggcctaatg cccataaaga tgctgtcaat aaattcgttg
     4981 tgtcgatacc tgaaaaaata cattgcgatt agcagtatta ttataaagga tggaaggacc
     5041 gagccaaggg ttgaggcaag agctccccta aaacccctta ttctgtagcc aacatacacg
     5101 cttgtgttaa gggccaccga ccccggggaa gcctgagaca aggcgattgc atccataaac
     5161 tcctcctcgc tgatcagttt cttgatgtcc acaagctcct tctggatgat tggcagcatt
     5221 gcatagcctc caccgaatgt gaaggcgcct atcttgaaaa atgttgtaaa aagacttaaa
     5281 gacattcctc cacctcctct agaagaataa tacacccagg gctcctgcaa gtacaatccc
     5341 gaatatgggg ttaagatccc tgaaggttac aagtacaaac aagagtattg ctataaggac
     5401 agatttaagg tcaacaaatg tgtttcctcc tacgcttacg gccgcagccg caatcagccc
     5461 caatacaaca ggccttatac ccttgaatat ccagtttaca tagggcgatt tcttgaac
//
LOCUS       DASRQF010000027         5497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_106037, whole genome shotgun sequence.
ACCESSION   DASRQF010000027 DASRQF010000000
VERSION     DASRQF010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106037"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VFD79_00755"
     CDS             <1..622
                     /locus_tag="VFD79_00755"
                     /inference="COORDINATES: protein motif:HMM:NF020091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HZH92603.1"
                     /translation="LSNRETYEIMTPESVGLSNNNLVLGKHSGRHAFKDKVVSLGYEI
                     DDDQINIIFAQFKDLADKKKQIFDRDIEILLTRGIKETRDYYKLERFVVNSGNTITTT
                     ASVVLDTPEGIREGISFAQGPVDASFKAIEQCVHKDITLEDYSLQSITEGTDALGYAV
                     VKLRYKDKHFVGRDASIDVVEASISAYINAVNKLMQEEETHQEMAV"
     gene            637..1905
                     /gene="leuC"
                     /locus_tag="VFD79_00760"
     CDS             637..1905
                     /gene="leuC"
                     /locus_tag="VFD79_00760"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HZH92604.1"
                     /translation="MGMTMTQKIMAAHSGRERVAAGDFIEADLDMVLGNDITAPPAIA
                     EFGRMGSERVFHRDKVALVPDHFAPNKDIKAAQQCKIMREFAHSQEITNYFEIGEMGI
                     EHALLPEKGIVVAGDAVIGADSHTCTYGALGAFSTGVGSTDMAAGMSTGKAWFKVPSA
                     ISIVLRNKPGEWVTGKDIILHIIGMIGVDGARYKSMEYSGEGVGHLSMDDRFTMANMA
                     IEAGAKNGIFPVDKVTEAYMGGRAKREWKVYGPDEDADYDQVIEIDLSKVRPTVSFPH
                     SPDNTHTIDNVPRVGIDQVVIGSCTNGRLDDMRMAARILKGRKVARGVRTIIFPATQE
                     IYLQSVREGLIEIFIEAGAIVSTPTCGPCLGGHMGILAEGERAVSTTNRNFIGRMGHH
                     TSEVYLASPAVAAASAVSGFIQNPEDLEVV"
     gene            1906..2412
                     /locus_tag="VFD79_00765"
     CDS             1906..2412
                     /locus_tag="VFD79_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HZH92605.1"
                     /translation="MKSQGRVIKFGDNIDTDLIIAARYLSTADPKDLAQHFMEDLDPE
                     ISKRVKEGDIVVAGRNFGCGSSREHAPIAIKGAGVSCVIAESFARIFYRNSFNIGLPI
                     IESPEASRDIEDGDQIKVDYETGEITNITTGKKYSGEAYPPFIRRIIENGGLVGYVKA
                     GGAEGHDI"
     gene            2402..3487
                     /gene="leuB"
                     /locus_tag="VFD79_00770"
     CDS             2402..3487
                     /gene="leuB"
                     /locus_tag="VFD79_00770"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HZH92606.1"
                     /translation="MTYRIAVARGDGVGPEVVESAIQVLERVSEVFEIGFEFQEVLVG
                     GAAYEATGSPLPEESLNTIKNCDSVLFGAVGGDRWDGLPHEMRPEAAILGLRKALKAY
                     SNLRPGILYPQLKEASPLKNHIVENGFDICVVRELTGGIYFGRKKTWEDEGGERAEDV
                     EEYSRMEIERIARKAFQTAMGRRRKVTSVDKANVLHTSRLWRRVVDEVHLDYPEVDLN
                     HMYIDNACMQAVRDPSQFDVILTSNIFGDILSDEISMLTGSIGLLPSASMGDGKEGIY
                     EPIHGSAPDLAGLDRVNPIGTILSAAMMLDHSFGLPDAARSIEDAIKKVFDEKYHTED
                     VFMEGGKTVGTKEITRLIAQNIENGAN"
     misc_binding    3574..3843
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3913..4143
                     /locus_tag="VFD79_00775"
     CDS             3913..4143
                     /locus_tag="VFD79_00775"
                     /inference="COORDINATES: protein motif:HMM:NF025087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HZH92607.1"
                     /translation="MKRTLNAMVKNGPETLMRITGLMRKRGCQLEGIVYEVGLNDALS
                     KLTLTVGAESEDFMNNMILQMKRFQDVYEVTE"
     gene            4171..5163
                     /gene="ilvC"
                     /locus_tag="VFD79_00780"
     CDS             4171..5163
                     /gene="ilvC"
                     /locus_tag="VFD79_00780"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HZH92608.1"
                     /translation="MAKMFYENDCDLNLLKDKKVAVIGYGSQGHAHALNLMESGVDVR
                     VGLYEGSKSWERAQEAGLKVMTSGEATEWADVVMLLVNDEKQVDLYENSVKGNLSPGN
                     YLAFAHGFNIHYGQIVPPEDVNVFMVAPKGPGHTVRSQYQEGKGVPCLVTTHQDPSGD
                     TMDVALAYAAGIGGARAGVLQTTFKEETETDLFREQAVLCGGVTELMKAGFEVLVEAG
                     YEPESAYFECLHEMKLIVDLINQGGLSYMRYSVSDTAEYGDYSVGRRIITEDTRNEMR
                     KVLGEVQDGTFAKNWILENKANRPSFTARRRMEQDHQMEVVGRDLRKMMSWLKK"
     gene            5218..>5497
                     /locus_tag="VFD79_00785"
     CDS             5218..>5497
                     /locus_tag="VFD79_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004814790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HZH92609.1"
                     /translation="MKLTGADVVMECLLREGVDTIFGYPGGAVIPLYDSLTRYRESFR
                     HILTSHEQGASHGADGYARSSGKTGVCFATSGPGATNTVTGIATAYSDS"
BASE COUNT         1653 a         1066 c         1590 g         1188 t
ORIGIN      
        1 ccttagcaac agggaaacct acgagataat gactccggaa tccgttggat tgagcaacaa
       61 caatctggtc cttggcaagc attcaggcag acatgcattc aaggacaagg tggtttcact
      121 tggatatgaa atagacgatg accagatcaa cattatcttc gcccagttca aggacctggc
      181 agacaagaaa aagcagattt ttgacaggga cattgagata ctcctaacca gagggataaa
      241 ggagacaagg gactactaca agcttgagag gtttgttgta aacagtggaa acacaataac
      301 aaccacagcc tccgtggtct tggatacccc agagggaatc agggagggaa tctcatttgc
      361 acaagggcct gtggatgcct cattcaaggc aattgagcag tgcgtccaca aggacataac
      421 ccttgaggac tattccctcc agtccattac agagggaact gatgcattgg ggtatgctgt
      481 ggtgaagcta agatacaagg acaagcactt tgtgggcagg gatgccagca tagatgtggt
      541 ggaggcaagc ataagtgcct atataaacgc agtgaacaag ctgatgcagg aagaggagac
      601 acaccaggaa atggctgtat agaaagggtg atttggatgg gaatgacaat gacacagaag
      661 ataatggcag cacattcagg aagggaaagg gtggctgcgg gagatttcat agaggcagac
      721 cttgatatgg tccttggcaa tgacataaca gcacctccgg caatagcaga gtttgggagg
      781 atggggtcgg agagggtatt tcacagggac aaggttgccc tggtacctga ccattttgct
      841 ccaaacaagg atatcaaggc tgcccagcag tgcaagatca tgagggaatt tgcccacagt
      901 caggagataa caaactactt tgaaatcggg gaaatgggaa ttgagcatgc acttctgcct
      961 gaaaagggga ttgtggtcgc aggggatgca gtcatagggg cggattccca tacctgtacc
     1021 tatggggccc ttggagcctt ttccacaggg gttggcagca ccgatatggc agctggcatg
     1081 tccacaggca aggcctggtt caaggtccca tcagcaattt ccatagttct tagaaacaag
     1141 ccaggtgagt gggttaccgg caaggatata atccttcata tcatcggtat gataggggtt
     1201 gatggagcca ggtacaagtc catggagtac tcaggtgagg gagtcggaca cctttcaatg
     1261 gatgacaggt tcaccatggc caatatggcg attgaggcag gtgcaaagaa cgggatattc
     1321 ccggtggaca aggtcactga agcctatatg ggtggccggg caaaaaggga gtggaaggtc
     1381 tacgggccag acgaggatgc ggactatgac caggtcattg aaatcgacct gtcaaaggta
     1441 aggccaacgg tctcatttcc acacagcccg gacaacaccc ataccattga caatgttcca
     1501 agggtgggga ttgaccaggt cgtgatcggg tcctgcacaa atggaaggct ggatgacatg
     1561 aggatggctg caaggatcct gaagggtcgg aaggttgcaa ggggtgtcag gaccataata
     1621 tttcccgcaa cccaggaaat ctacctccag tctgtgaggg aaggcttgat cgagatattc
     1681 atagaggctg gagccattgt aagcactcca acctgtggac cctgtctggg aggacacatg
     1741 gggatactgg cagaaggtga aagggccgta tccacgacca acaggaactt tatcggcagg
     1801 atgggccatc atacatcaga ggtctacctg gcaagccccg ctgtagcggc agcctctgcc
     1861 gtaagcggct ttattcaaaa tccagaagat ttggaggtgg tctagttgaa atcccaggga
     1921 agggtcatta aatttggaga caacatcgac acagacctta taattgccgc aagatacctg
     1981 tcaacggcgg atcccaaaga cctggcccaa cactttatgg aggaccttga tcctgaaata
     2041 agcaagaggg tcaaggaggg tgatattgtt gtggcaggca ggaactttgg ctgcggatca
     2101 tccagggagc acgcacccat agccataaag ggcgccggag ttagctgtgt gatcgcggaa
     2161 agctttgcaa ggatattcta caggaattcc tttaacatag gtctgccaat aattgaaagt
     2221 ccagaggcgt ccagggacat tgaggatggt gaccagatca aggttgacta tgaaacaggt
     2281 gaaatcacaa atataactac aggtaaaaaa tacagtggtg aagcctaccc cccatttatc
     2341 agaaggataa ttgaaaatgg aggattggta ggatacgtaa aagcgggagg agcagaggga
     2401 catgacatat aggatagcgg tggcaagagg tgatggtgtg ggcccggagg ttgttgaaag
     2461 tgcaatccag gtccttgaaa gggtttcaga ggtttttgag attggatttg agttccagga
     2521 ggtccttgtg ggaggggcag cctatgaggc cacaggaagc cccctgcctg aagaaagtct
     2581 taataccata aaaaactgtg acagtgtgct cttcggtgcg gttggtggag acagatggga
     2641 tggtcttccc catgaaatga ggcctgaggc ggcaatcctt ggactgagga aggcccttaa
     2701 ggcctattca aaccttagac ccgggatcct ctacccccag ctgaaggagg cttcacctct
     2761 taagaaccac attgtggaaa atggatttga catatgcgtg gtaagggagc ttactggggg
     2821 gatatacttt gggaggaaaa aaacctggga ggatgaaggt ggtgaaaggg cagaggatgt
     2881 tgaggagtac agccgcatgg aaattgagcg gatagccagg aaggccttcc agactgccat
     2941 ggggaggaga aggaaggtaa ccagtgtcga caaggcaaat gtccttcaca catcaaggct
     3001 ttggagaagg gttgttgatg aggtccatct tgattatcct gaagtggacc tgaaccacat
     3061 gtatatagac aatgcatgta tgcaggctgt tagggaccca tcccagtttg atgtgatcct
     3121 gacatcaaat atctttgggg acatcctttc agatgaaata agcatgctga cagggtccat
     3181 aggcctgctt ccctcagcca gcatgggtga tgggaaagag ggcatatacg aacctataca
     3241 cgggtctgcg ccagacctgg caggactgga cagggtcaac ccaataggga caatcctttc
     3301 agcagcaatg atgcttgacc attcctttgg actgccagat gccgcaaggt ccattgagga
     3361 tgcaataaaa aaagtttttg atgaaaaata ccacactgaa gacgttttca tggaaggtgg
     3421 aaaaaccgtg ggaacaaagg agattacaag gcttatagcc cagaacattg aaaatggtgc
     3481 caattgaaaa tattctaaat aatttaaatt atttaaaaaa ggtgttgaca cctttgattt
     3541 taagttatag aatgggttta atttaaatca caaacaagag caatgacgga gacaaaactg
     3601 tagagagtct gataacagag aactgatgga tggtgagagt caggatcaga ggacgcagga
     3661 atgatcactc cggagctgtc aaggcgaaat ggaaaaagta gctgcgaccg ggaaccttcc
     3721 aaatggaaga tgcgccaccg ttacatggct atggttaaag gcttgtcctt ggacctgaag
     3781 agtcagtctg cgtgagcaga ttgtaaacca gggtggtacc gcgaatatta tctcgtccct
     3841 gaaatacata taagtatttc agaggcgaga ttttttatac ccaaaaacaa aaaataaagg
     3901 gaggaaatga aaatgaaaag aactttaaat gctatggtga agaatggacc ggagacccta
     3961 atgagaatta caggacttat gagaaaaaga ggctgtcagc tggagggaat cgtatatgag
     4021 gtgggtctta atgatgcgct gtcaaagctt acactgaccg ttggagctga aagcgaggac
     4081 tttatgaaca atatgatcct tcagatgaaa aggttccagg atgtttacga ggttacagaa
     4141 taaattacat aacaggggag agatggaaaa atggcgaaga tgttctacga aaacgactgt
     4201 gatttgaatt tgcttaaaga taagaaggtg gctgtaatag gctatggtag ccagggtcat
     4261 gcacatgcac ttaaccttat ggaatccggt gtggatgtaa gggttggcct ttacgagggc
     4321 agcaagtcct gggaaagggc acaggaggca ggacttaagg ttatgacctc aggcgaggca
     4381 acggagtggg ccgatgtggt aatgctgctt gtaaacgatg aaaagcaggt tgacctttat
     4441 gaaaacagtg tgaaggggaa cctgtcacct ggaaactacc tggcctttgc ccacggcttc
     4501 aacatccatt acggacagat tgtaccaccg gaggatgtga atgtgtttat ggtagctccc
     4561 aaggggcctg gacacactgt tagaagccag tatcaggagg gaaaaggggt gccttgtctg
     4621 gttacaaccc accaggaccc aagcggggat accatggatg ttgcccttgc atatgcagca
     4681 ggaattgggg gagccagagc cggtgtactt cagaccacct tcaaggagga gactgaaaca
     4741 gaccttttca gagagcaggc agtactttgc ggtggagtta cagaactgat gaaggcaggt
     4801 tttgaagtac ttgtggaggc gggctatgag cctgaaagcg catactttga atgtctgcac
     4861 gagatgaagc tgattgtcga cctgataaac cagggaggac taagctacat gaggtattca
     4921 gtaagtgata ctgcagagta tggagactat tcagtaggca gaagaatcat aaccgaggat
     4981 accagaaatg aaatgagaaa ggtccttgga gaggtccagg acgggacctt tgccaagaat
     5041 tggatacttg agaacaaggc gaacagaccg tccttcacag ccagaagaag gatggagcag
     5101 gaccaccaga tggaggttgt tggaagggac cttagaaaga tgatgagttg gttaaagaaa
     5161 taggatgaat catttgtttt atgagagctg taatgactag aatacagggg tgaggaaatg
     5221 aagttgacag gtgcagatgt agtgatggag tgcctcctta gggaaggcgt ggacaccata
     5281 ttcggatatc caggaggtgc tgtgattccc ctttacgact ccttgacaag gtatagggag
     5341 agtttcagac atatcctaac aagccacgag cagggagcat cccacggggc agacggatat
     5401 gccaggtcaa gcggaaagac aggggtatgc ttcgcaacct cagggccagg tgccacaaat
     5461 accgtcactg gaatagcaac agcatattcg gattcag
//
LOCUS       DASRQF010000028         4078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1862089, whole genome shotgun sequence.
ACCESSION   DASRQF010000028 DASRQF010000000
VERSION     DASRQF010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1862089"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VFD79_00790"
     CDS             <1..811
                     /locus_tag="VFD79_00790"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HZH92610.1"
                     /translation="RLGGDRVEVRYLLPAGTEAHSYEPTPQDMVALLNADVFLYTGEF
                     MEPWTRNIVENLKEEDVLVVDLSQGIPLLTSEDHDHDHDHGEEDDHEEGIDPHYWTDP
                     NMAILMVQSISDALSQVDPENEEAYISEEIELIDELTKLDGDIREALSRTKSRTILSG
                     GHFAFGYFAREYGLEHMSPYSGFSPNAEPTPQRITALIKTIEETGANAIYHEELIDPK
                     VAQVIVDETGVRLLLLHGAHNISKEDLESGKTYMEIMYDNLENLKEGLGYE"
     gene            804..1538
                     /locus_tag="VFD79_00795"
     CDS             804..1538
                     /locus_tag="VFD79_00795"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HZH92611.1"
                     /translation="MSNRLIEVENLSVYYGQQQVLKDVSFVIEQGDYVGLVGANGSGK
                     TTLVKSILGLVPISNGTITYHGSGGVHKGVGYLPQVAVTGNTLFPGEVHEIVAVGLLG
                     KKRFPKRITWEDNRRIDEILKRLDIYQLKHNKIGDLSGGQQQRVLLARAMVSSPRLLI
                     LDEPTSALDPKVRTEFFSLIRKINQEEGTSIILVSHDLSSIKKCSSKIMLLDRELLYY
                     GPTVDFNMNVNFVEGIHDHDHERVVS"
     gene            1538..2362
                     /locus_tag="VFD79_00800"
     CDS             1538..2362
                     /locus_tag="VFD79_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HZH92612.1"
                     /translation="METIELIREAMSLAFMQRAMIVGTAIALSSALLGIFLVLKKYSM
                     IGDGLAHVSFATVAIALLLGASPLLVSIPIVTIASIFIMQLNERAGIHGDAAIGLVSS
                     FSVALGVLISSVAGGFNVDLFSYLFGSILAIKETDMYLSIILAGLIISLIILFYHDLF
                     AVTHDEAFASVIGIGTKRMNYLIATLTSITIVLGIRVVGTMLISSMIVFPTVTALQVS
                     KSFKSTIIISAIVSIIAVTTGVIGSYVFNFPSGASIVMINAFLFILFLGMKRFVRI"
     gene            2381..2827
                     /locus_tag="VFD79_00805"
     CDS             2381..2827
                     /locus_tag="VFD79_00805"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HZH92613.1"
                     /translation="MSSDKIRELFKSRGIKNTQQRHLIYEFLSRKKQPITAEEIYMVL
                     SKKEKTKMNLSTVYRILDTFTKKGLVQKSGINIEGKSTYEINHMDHRHHLVCVECNKI
                     LPIKGCPLEGYERYLAETTGFAILEHHLEIKGICPDCQDESNGKDK"
     gene            2929..3153
                     /gene="secG"
                     /locus_tag="VFD79_00810"
     CDS             2929..3153
                     /gene="secG"
                     /locus_tag="VFD79_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HZH92614.1"
                     /translation="MTTLFSVLMLISSGTLIGSILMQESKSEGLGAISGGSDSMFGKS
                     VGTSRQAMLRKVTIVSAVIFMISAVALAAV"
     gene            3170..>4078
                     /locus_tag="VFD79_00815"
     CDS             3170..>4078
                     /locus_tag="VFD79_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HZH92615.1"
                     /translation="MSFALYAAPIVGVVALLFAFYKASSINKVSPGNDRMKEIAAYIQ
                     ERSMAFLSRQYKSLGIFVAVLFVILAVVIGITTAITFIFGAAFSIMAGFFGMRVATKA
                     NVRTANAAMESGMTKALNVAFSGGAVMGMSVVGLGLLGIGVSYIVFRDPAIITGFGLG
                     ASSIALFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGDVAGM
                     GADLFESYVGAIVSAVTLGLIAYGESGVFFAFGLAAVGILASIVGVFFVRGDGDPQKS
                     LDKGTLVSSLLTMVIGALLSIMILGSF"
BASE COUNT         1215 a          748 c         1014 g         1101 t
ORIGIN      
        1 aagactgggt ggagacaggg tggaggtaag atacctgctt ccagcaggaa cggaggccca
       61 ctcctacgag cccactccac aggatatggt ggcactattg aatgcggatg tctttctcta
      121 cacaggtgag ttcatggaac cctggaccag gaacatagtg gaaaacctca aggaagagga
      181 tgttttggtt gttgacctaa gtcagggtat tccgctttta acatctgaag accatgacca
      241 tgaccatgat catggtgaag aggatgacca tgaggaggga attgatcccc actactggac
      301 tgacccaaat atggccatct tgatggtaca aagcattagt gatgccctgt cacaggtgga
      361 ccctgaaaat gaagaagcat atatttcaga ggaaatagag ttgattgatg aacttaccaa
      421 gcttgatggt gatatcaggg aggccttgtc aaggacaaaa tccaggacaa tactttcagg
      481 aggccatttt gccttcggtt attttgcaag agaatacggt cttgagcaca tgtctccata
      541 ctcagggttt tctcccaatg ccgagcctac accccagagg ataactgcct tgataaaaac
      601 cattgaggaa acaggtgcaa atgcaatata tcatgaggag ctgattgacc caaaggtagc
      661 tcaggtcatt gttgatgaga caggtgtaag gctgcttctg ctccatggag cacataacat
      721 atccaaggaa gacctggaat ctggcaagac ctatatggaa ataatgtatg ataatcttga
      781 gaatctgaaa gagggattgg gatatgagta ataggctaat tgaagtggag aatctttccg
      841 tatactacgg ccagcagcag gttctaaagg atgtttcctt tgtcatagag caaggtgact
      901 acgtggggct ggttggggca aatggatcag gaaagacaac ccttgtaaaa tcgatacttg
      961 gtttggtgcc catttcaaat ggtaccataa cctaccatgg atcgggagga gtccacaagg
     1021 gtgtgggtta ccttccccag gttgcggtaa ctggaaatac tctgtttcca ggtgaggtac
     1081 acgaaattgt agcagtgggt ctgctgggca agaagcggtt cccaaagagg ataacctggg
     1141 aggataaccg aagaattgac gaaatcttga aaaggcttga catatatcag ctcaagcaca
     1201 ataagattgg agacctatca ggtggacagc agcaaagggt tcttctggca agggcaatgg
     1261 taagcagccc aaggcttcta atccttgacg agcctacaag tgccttggac cccaaggtcc
     1321 gaacagagtt cttcagcttg ataaggaaga taaaccagga ggagggaaca agtatcattc
     1381 ttgtttccca tgatctgagc agtatcaaga aatgctcatc aaaaattatg cttttggacc
     1441 gggaactatt atactatgga ccaacggttg actttaacat gaacgtaaac tttgtagagg
     1501 gaattcacga ccatgatcat gaaagggtgg tgagctgatg gagacaattg aactcataag
     1561 ggaggccatg agtcttgcct ttatgcagag ggcaatgatt gttgggacag ccattgcact
     1621 aagcagtgcc ctgcttggga tatttctggt tttaaaaaag tactcaatga ttggggatgg
     1681 actggcccat gttagctttg caactgtagc catagcactc ctattgggag catcgcccct
     1741 ccttgtttca ataccaattg taaccatagc ctcaatattc atcatgcagc tgaatgaaag
     1801 ggcaggaatt catggggacg ctgcaatcgg actagtttct tcattttcgg ttgccttggg
     1861 tgtattgatt tcaagtgtgg ccggaggatt caatgtggac ctgttcagtt atctttttgg
     1921 aagcatactt gccataaagg aaaccgacat gtatctttca atcatacttg caggattgat
     1981 aatatcactg ataatactct tctaccacga cctgttcgca gtgacacatg atgaagcctt
     2041 tgcaagtgta attggaattg ggacaaagcg tatgaactac cttatagcca ccttgacatc
     2101 cataaccata gtccttggta taagggtggt tggaacgatg cttatctcaa gtatgattgt
     2161 atttccaacg gtaactgcac tacaggtatc aaagagcttt aagtcaacaa ttataatttc
     2221 ggcaatagtt tcaatcattg ctgttacaac aggagtaatt ggatcctacg tgtttaactt
     2281 cccatcaggt gccagcattg taatgatcaa tgcatttctc ttcattttat ttttgggaat
     2341 gaagagattt gtcaggatat gaccatggga ggtgtcaggc atgagcagtg ataaaatacg
     2401 ggaactattc aagtcaagag gtatcaagaa cactcagcaa aggcatttga tctatgaatt
     2461 tctaagcagg aaaaagcaac ccattactgc cgaagaaatc tacatggtcc tttcaaaaaa
     2521 agaaaaaacc aaaatgaacc tgtcaacagt gtacagaata ctggatacct ttacaaaaaa
     2581 aggattggtc cagaaatccg gaataaatat cgaggggaaa tccacttacg agatcaatca
     2641 tatggaccat aggcaccacc ttgtctgtgt ggagtgcaat aaaatcctac ccataaaggg
     2701 ttgtccactg gaaggttatg aaaggtacct ggcggagacc acagggtttg ccattctgga
     2761 acaccatctt gagatcaagg gaatttgccc ggactgtcag gatgaaagca atggaaaaga
     2821 taagtaaagt atgggttaaa ttccattgct tttaaatttt gcctatgtta aaatggtaat
     2881 gttatagact tgaatattaa atattttttc atgtacagga ggtgtagtat gacgacttta
     2941 ttctcagttt taatgttgat ttccagtggt accctcatag gaagtattct tatgcaggaa
     3001 agcaaatcag aaggtttagg cgctattagc ggtggcagcg attccatgtt tggcaagagt
     3061 gtgggaacaa gccgacaggc aatgctaagg aaggtaacaa ttgtatctgc agtgatattc
     3121 atgatttcag ctgttgcttt ggcagcggta taataaggag gaaggtaaaa tgagttttgc
     3181 tttatatgcc gctccaatag tgggagtggt tgctttgtta tttgcatttt acaaagcatc
     3241 atcaatcaat aaggttagcc ctggtaatga caggatgaag gaaatcgcag catatattca
     3301 ggaaaggtca atggcattct tgtcaagaca gtacaaatcc ctgggtattt ttgtggcagt
     3361 tctatttgta atacttgccg tagtgattgg tattacaact gcaattacat ttatttttgg
     3421 agcagcattc tccattatgg caggtttctt tggcatgagg gttgcaacaa aggcaaatgt
     3481 aagaacagca aatgcagcaa tggaaagcgg aatgacaaag gcactgaatg ttgctttctc
     3541 aggtggggca gtaatgggaa tgagcgttgt aggtcttggt cttctgggaa ttggcgtatc
     3601 atatatagtt tttagagacc ctgcaataat aaccggattc ggacttggag catcatcgat
     3661 cgcgctgttt gccagagtag gtggaggtat ttacaccaaa gccgctgacg ttggagctga
     3721 ccttgtaggt aaggttgagg ctggaatacc agaggacgac ccaagaaacc ctgcagttat
     3781 agcagataat gttggagaca acgtaggtga tgttgctgga atgggtgcag accttttcga
     3841 gtcatacgta ggagcaatcg tatcagcagt aacacttgga cttatagctt atggagagag
     3901 cggagtattc tttgcatttg gtcttgcggc agttggaata cttgcatcaa tagttggagt
     3961 attctttgta agaggagatg gagacccaca gaagtcactt gacaagggaa cacttgtaag
     4021 ttcattgttg acaatggtaa ttggagcctt actaagcatc atgattctag gatcattc
//
LOCUS       DASRQF010000029         4598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1225897, whole genome shotgun sequence.
ACCESSION   DASRQF010000029 DASRQF010000000
VERSION     DASRQF010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225897"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VFD79_00820"
     CDS             complement(<1..475)
                     /locus_tag="VFD79_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HZH92616.1"
                     /translation="MNTRTIDGYKLKVILESAAIKLEANKDEVNSLNVFPVPDGDTGT
                     NMLLTMKSALKQGLGVEENDAHKVAQAASQGSLMGARGNSGVILSQLFRGFASGLEEK
                     AEIDIPTFALGLKKAADTAYKAVMKPTEGTILTVARECGEKAKSICDGRDDMVTFL"
     gene            complement(490..846)
                     /locus_tag="VFD79_00825"
     CDS             complement(490..846)
                     /locus_tag="VFD79_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HZH92617.1"
                     /translation="MPVKTKTEFGDLNINDNVFASLAGISAMESYGIVGMASKDAKDG
                     FFELLKSDSLTKGIKVYIKDNLITIDMYVILEFGVKISVVCKNIIDRVKFNIENFTGL
                     KVDKVNVFVQGIRVDK"
     gene            1035..1226
                     /gene="rpmB"
                     /locus_tag="VFD79_00830"
     CDS             1035..1226
                     /gene="rpmB"
                     /locus_tag="VFD79_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HZH92618.1"
                     /translation="MSKKCEICGKSKVFGNKVTFSNKKSSRTWAPNIRRVKAVVNGST
                     KRINVCTRCLRSGYVQRAI"
     gene            complement(1293..1928)
                     /locus_tag="VFD79_00835"
     CDS             complement(1293..1928)
                     /locus_tag="VFD79_00835"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HZH92619.1"
                     /translation="MKGLVAAGGRGIKRELLIGLCKEADIVVAADSGIYNLKEGACRI
                     DYLIGDFDSLTDTSILDAPGSSMEIIRFPVEKDKTDTELAIDFLALKGCSQITLVGAM
                     GSRMDHTISNIYLLRSFHQKGISIRIIDDNNEISYLTQERLLYKERGYYYSILPIGPD
                     GITVSLEGFHYPLKNELIEYGSSLGISNYLEDNEGRIIVHKGEGFLVKSKD"
     gene            complement(1939..2484)
                     /gene="thiT"
                     /locus_tag="VFD79_00840"
     CDS             complement(1939..2484)
                     /gene="thiT"
                     /locus_tag="VFD79_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HZH92620.1"
                     /translation="MEQKRSKVLMLTEGGMMIALSALLSYVRIYQAPYGGSVTAGSMI
                     PIMIFAIRWGLGPGLAVGATYGILDFILKPFFVHPLQLLFDYPMAFGSLGLAGIAYVL
                     AKHKGKSGYGSVMLGVALAIAGRLVFHVLSGVVFFAEYAGDQNPWIYSILYNASYLIP
                     ELIISLIVMVIVWKPIQKFIR"
     regulatory      complement(2565..2664)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(2738..3391)
                     /gene="rpe"
                     /locus_tag="VFD79_00845"
     CDS             complement(2738..3391)
                     /gene="rpe"
                     /locus_tag="VFD79_00845"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HZH92621.1"
                     /translation="MAKLAPSLLSADFSKLGEEIAQIEKGGADYVHLDVMDGSYVPNI
                     TFGAPVIKKIRDTTRLPFDVHLMVDRPERFIGDFADAGSDIITVHPEATIHLHRTLQS
                     IKEKGIRAGVSLNPSTPLNVLDHIMDDIDLILVMSVNPGFGGQSYIPAMTDKIRKLRR
                     LISESGRDIILEVDGGIKLDNAKEIIDAGADMIVVGSGIFSALDVSKRVEDFKNIIA"
     gene            complement(3393..4271)
                     /gene="rsgA"
                     /locus_tag="VFD79_00850"
     CDS             complement(3393..4271)
                     /gene="rsgA"
                     /locus_tag="VFD79_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HZH92622.1"
                     /translation="MIEGTIIKGIGGFYYVQTENGVVQCRARGIFRETNITPLVGDRV
                     RIRISEEDNNGYVEEIFERESVLLRPPVANVTQAVIVSSIKDPELNTWLLDRMLVMAE
                     EQELEVLICINKCELDPDGAKQMGEIYKGAGYDTILTSVKQKQGLEELKDFMEDHISV
                     FAGPSGVGKSSLLNSINSNYDLEIGKISLKTSRGKHTTRHVELLRLNKNSFVLDSPGF
                     SSLNLDFVEDEGQLRFYYKDIEQYNGRCKFSSCIHVNEPGCAVKEAVESSTISSERYK
                     NYLMMIEEIKKRKRRY"
     gene            complement(4274..>4598)
                     /locus_tag="VFD79_00855"
     CDS             complement(4274..>4598)
                     /locus_tag="VFD79_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92623.1"
                     /translation="LYVSSGPEPNAEPEPDPVPGDEPQDPGNDQPPVGEESNFTFTLT
                     PFTDREETEVTIFRKQGGDPTMVYSSTHKASDGSFQVTVTGLPGAQFEVYYDGIYQFT
                     RVKEG"
BASE COUNT         1295 a         1084 c          777 g         1442 t
ORIGIN      
        1 caagaaaggt taccatatca tctctgccat cgcagattga ctttgccttc tcaccgcatt
       61 ctcttgcaac ggttaaaatt gttccttcgg ttggcttcat tactgctttg tatgcagtat
      121 cagccgcctt tttcaaaccc aatgcaaatg ttgggatgtc tatctccgcc ttttcttcaa
      181 gaccagaagc aaagcctctg aaaagctgtg atagtatgac accagagttt cccctggctc
      241 ccattaaaga accctgactg gcggcctgtg ccaccttatg ggcatcgttc tcctctacac
      301 caagcccctg cttcaaggca gatttcattg tcagcagcat atttgttcct gtatcaccat
      361 caggtacagg gaaaacatta agtgaattca cctcatcctt attggcttca agttttattg
      421 ccgcactttc caagataacc tttaacttat atccatcaat tgtcctagta ttcatcattg
      481 acctcctaat tacttgtcga ctcggattcc ctggacaaat acattaacct tgtccacctt
      541 caatccggtg aagttctcta tattgaattt aaccctgtca attatgttct tgcaaaccac
      601 agagatttta actccaaact ccaatataac gtacatatct attgtaataa ggttgtcctt
      661 gatatatact ttaatcccct tagtcagtga gtctgatttc aatagttcaa agaaaccatc
      721 ctttgcatcc tttgaagcca tgcctacaat tccgtagctt tccattgctg atattccagc
      781 tagagatgca aacacgttat catttatatt cagatcacca aattcagtct ttgttttgac
      841 tggcataacc attcctcccc gtgttttact cttatccata ttattttaca tgattaaggc
      901 tgattgttca actaaaacaa gaagatttca taatattagg ttgcaattct ataatctctt
      961 gtgttaaaat ataatatgtt gcggagaatg aatattaaaa cattttacct gttaggaatt
     1021 aaggaggtgt tttcatgtct aagaaatgtg aaatttgcgg aaaaagtaag gtatttggaa
     1081 ataaggtgac cttctcaaat aaaaagagtt caagaacatg ggctccaaat ataagaagag
     1141 taaaggcagt tgtaaatgga tcaaccaaga gaatcaacgt atgcacaaga tgcttgagat
     1201 ctggttatgt tcaaagagcc atttaatcca tacttacaag agtaaaagct tattatgaga
     1261 cgatcataat aagctttttt cttgttgtag acttaatctt tggacttgac cagaaaacct
     1321 tctcctttgt gaacaattat ccttccctca ttatcctcca gataattgct gatccccaaa
     1381 cttgaaccat actctataag ctcatttttc agaggatagt gaaagccctc caggctcact
     1441 gtgatcccat caggccctat aggaagtatt gaatagtaat aacccctctc cttataaaga
     1501 agcctttctt gggtgagata ggaaatctca ttgttgtcat caattattct gatacttatt
     1561 cccttctggt ggaatgacct caaaaggtat atgtttgaaa tggtatgatc catcctgctc
     1621 cccattgccc ccacaagagt gatctgggag cagcctttaa gggctagaaa gtcaattgca
     1681 agctcagtgt ccgtcttgtc cttctctaca gggaatctaa tgatctccat ggaggaacca
     1741 ggggcatcca gtatgctggt gtcagtgagt gaatcaaaat cacctatcaa ataatcaatc
     1801 ctacatgccc cttctttgag attatatatt ccactgtcag ctgccacaac tatatcagcc
     1861 tctttgcaaa gaccaatcaa aagttccctt tttatgcctc ttcctcctgc tgctaccaat
     1921 cccttcataa aaccatcctt atcttataaa cttctgtatt ggtttccata ctatgaccat
     1981 gacaatcaat gaaattatca actctggtat aaggtagctt gcattataga ggattgagta
     2041 aatccatgga ttctggtctc cagcatattc agcaaaaaac acaactccgg acaatacgtg
     2101 aaataccaat cttcctgcaa ttgccaatgc cactccaagc atcacagatc catatcctga
     2161 cttgccttta tgctttgcta gaacataggc aattcctgcc aagcccaatg aaccaaaggc
     2221 catgggataa tcaaaaagca gctgcaatgg atgaacgaaa aatggcttaa gtatgaagtc
     2281 caaaatacca taggtcgctc caactgccag tccaggacct agaccccatc taatggcaaa
     2341 aatcattatt ggtatcatgc tacctgctgt aacgctccca ccataggggg cctggtaaat
     2401 ccgcacatag ctcagcaacg cagataatgc aatcatcatg ccaccctcgg tcaacatcaa
     2461 aactttactt cttttctgtt ccatattata ttaaacctcc tttaatggtt gaatataaca
     2521 gcaaaagatg gtacaaaaaa actcattgct tggagcaatg agtataattt ctatacacac
     2581 ttgcctccgc tagaattacc tagttcaggt cttaagggtc gagatacttg atctctctca
     2641 gctaaagctc ccccagtgct gtaaatattc ttttgtcata tatagaataa cacattgatt
     2701 tgaaattctc aataattatg tgcatattct gataaattta ggcaattata ttcttaaaat
     2761 cctctactct tttggaaaca tccagggcgc tgaagattcc agatccaact acaatcatat
     2821 cagctcctgc atctatgatt tccttggcat tgtcaagctt gataccacca tctacttcaa
     2881 gtattatatc tctacctgac tctgatatca atcttctaag tttcctgatc ttatcagtca
     2941 ttgccgggat gtagctctgc cctccaaagc ctggattgac tgacattacc agtatcaggt
     3001 caatatcatc cattatgtgg tccaatacat tcaagggtgt ggaagggttc agtgaaacgc
     3061 ctgccctaat tcctttttcc tttatggact gaagggttct atggagatgt atggtggctt
     3121 cgggatggac tgttatgata tctgagcctg catcagcgaa atcaccaata aacctttctg
     3181 gtctgtcaac catcaaatga acgtcaaacg gcaaccttgt agtatcacgt atcttcttga
     3241 taactggggc tccaaaggta atgtttggta cataactacc atccatgaca tccagatgta
     3301 catagtctgc cccgcctttc tcaatctgag caatttcttc tccaagcttt gaaaaatctg
     3361 ctgaaagcag tgaaggtgcc aactttgcca tactagtatc tcctctttct tttcttaatt
     3421 tcttctatca tcatcaaata atttttgtac ctttcagagc taatggtgct tgattcaaca
     3481 gcttccttaa ctgcacatcc tggctcgttc acatgtatgc agctggaaaa cttgcatcga
     3541 ccattgtatt gctcaatatc tttgtaatag aacctaagct ggccttcatc ctcaacaaag
     3601 tcaagattaa gtgaactgaa gccaggacta tccaaaacga atgaattttt gttcaacctt
     3661 aaaagttcaa catgcctggt cgtatgctta ccccttgatg ttttcaagct tatcttccct
     3721 atttccaaat catagttgga atttattgaa ttcagcagtg atgacttccc tacaccggat
     3781 ggtccggcaa aaacacttat gtgatcctcc atgaagtcct ttagttcctc aagaccctgc
     3841 ttttgtttaa cgctggtcag gatggtgtcg tatccagctc ccttgtatat ttcccccatc
     3901 tgttttgcac catcggggtc tagctcgcat ttgttgatgc atattaatac ttccaattcc
     3961 tgttcctcag ccataacaag catcctatcc aaaagccatg tgttcaactc tggatccttg
     4021 atgcttgata caataactgc ttgtgtcaca ttggcaaccg gtggtctcaa aagaaccgat
     4081 tccctttcaa atatctcctc aacataacca ttgttgtctt cttcgcttat tctaatccta
     4141 accctatctc caacaagtgg ggttatgttt gtttctctga aaatacccct tgccctgcat
     4201 tgaaccacac cattttcggt ctggacataa tagaatccgc caataccttt tatgatagtt
     4261 ccttcaatca taattaaccc tcttttactc ttgtgaactg atatatccca tcataataga
     4321 cctcaaattg agctccgggc aaccctgtta cagtaacctg gaatgatccg tcactcgcct
     4381 tgtgtgtaga cgaatagacc atggtgggat caccgccctg ttttctgaaa atggtaactt
     4441 ctgtttcctc tctatccgtg aaaggtgtca atgtgaatgt aaagttgctt tcctctccaa
     4501 caggaggctg gtcattacct ggatcctgtg gttcgtctcc aggaactgga tctggctctg
     4561 gttcagcgtt tggttctggc cctgatgaaa catacagg
//
LOCUS       DASRQF010000030         3439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_225086, whole genome shotgun sequence.
ACCESSION   DASRQF010000030 DASRQF010000000
VERSION     DASRQF010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225086"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /locus_tag="VFD79_00860"
     CDS             complement(<1..1274)
                     /locus_tag="VFD79_00860"
                     /inference="COORDINATES: protein motif:HMM:NF019194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HZH92624.1"
                     /translation="MNLLFDKFKEVSLSVLPITIIVLMLNFTLTPLDGTMLLTFIIGA
                     FLIVVGLTLFLIGIDIGIAPLGKLLGATISTTGKVSIVAVSGLVLGFLISIAEPGLMV
                     LANQVDAVTDGGIRGTTLLVVVSFGLAVMVAVGMLRIVYNIPLYKVLTVLYGVTFILA
                     IFTPSQLLTIAFDTSGATTGILAVPFILALSVGVSALKKDSKAGEKDSFGLVSIASVG
                     AIMSVMVLSIFSDTGELSSTLQFEIPVAESLIGPFSSHLSGIAGESIMSLAPLLIIFL
                     LLQKVSFKLEKKAFSRFLKGFIYSFLGLVLFMVGVNGGFMNVGTIIGYSLASRDSYYF
                     LILMGFILGVVTMLAEPAVYVLTHQVEDVTSGYVKRRAVLTALALGGGLAIGLSMIRI
                     ILPGIQLWHYLIPGYALSIGLSYVVPKLFVGIA"
     gene            1471..2715
                     /locus_tag="VFD79_00865"
     CDS             1471..2715
                     /locus_tag="VFD79_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZH92625.1"
                     /translation="MNKYFDPKEKVYDITERYPETLDVFAANGFEHLKNEAMRKVMGK
                     TINLETACKTKKVNLQLFEQKLVDVIEQTRGSVDATLNQARENGDGEVSILGVLPCPV
                     RIPLMESFSKWLEDHEKDLDYRIDYELKAANIGVDWIRDEMEKASSHDIADLFMSAGF
                     DLFFDEKLMGKFKSQGVFQDMSGFESLNTDFDNDRISLKDPMGQYTTLAVVPAVFLVN
                     TEELDGREMPLSWEDLLSGEFENSISLPLNDFDLFNALLLSIHKEFGEDGIRRLGRAH
                     MRSMTPAEMVKSNIRNTSSGIPTVTVMPYLFAKMVREESPLKPVWPKEGAVISPIFLL
                     SKEDKRDKVKPLVDYFMSKEVGQMLATSGKFPSTHPEVENGLTEDQGFMWIGWDYINS
                     HPDLGALIRELMDIFNEEAGVK"
     gene            2716..3417
                     /locus_tag="VFD79_00870"
     CDS             2716..3417
                     /locus_tag="VFD79_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZH92626.1"
                     /translation="MNLVIFSGPPSSGKTSIILKTIEAMRKRDLQVGVVKFDCLSTDD
                     DILYEKAGIPVKKGLSGALCPDHYFASNIEEVVQWGRKTGLDLLITESAGLCNRCSPY
                     IKEIKGVCVIDNLSGINTPKKIGPMLKAADIVVITKGDIVSQAEREVFASKVNSVNPK
                     AMTMHINGLTGQGAFELSTLLYDEDRRLEAVTGMELRYPMPSALCSYCLGETRIGEAF
                     QMGNVRKIELGDMDE"
BASE COUNT         1106 a          702 c          812 g          819 t
ORIGIN      
        1 gcaataccca caaacagttt gggtacaaca tagcttaggc caatggaaag agcatagcct
       61 gggatcagat agtgccaaag ctgaatgccg ggcaaaataa tccttatcat tgacaggccg
      121 attgcaagac ctccaccaag agcaagggct gtaagtacgg ctcttctttt tacataacca
      181 cttgttacat cctcaacctg atgtgtaagg acatagacgg caggttctgc aagcattgtt
      241 acaactccca aaatgaatcc cataaggatg agaaaataat agctgtcccg agatgcaagt
      301 gaatagccaa tgatggttcc aacattcatg aatcctccgt ttacgcccac cataaagagg
      361 accaaaccaa ggaatgaata gatgaatccc ttgagaaacc tgctgaaggc tttcttctcc
      421 agtttaaagc ttaccttttg taggagcagg aaaatgataa gtaaaggagc cagggacatt
      481 atgctctcac ctgcaattcc cgacaggtgg gaagagaatg gtccaatcaa tgactcagcc
      541 acaggtattt caaattgcaa tgtggagctg agctcaccgg tatctgaaaa aatgcttaga
      601 accatcactg acattattgc tcccacagat gctattgata ccaatccaaa gctgtccttc
      661 tctccagctt tgctgtcctt tttcagtgcg gaaaccccaa ctgaaagagc cagaataaag
      721 ggaactgcca gaattccagt tgttgcccca gatgtgtcaa atgctattgt caaaagctgg
      781 gacggtgtaa agattgcaag aataaatgtc accccataaa ggactgtcaa taccttatat
      841 aggggaatgt tgtaaacaat ccttagcatt ccaacagcca ccataacagc gagaccaaag
      901 gatacaacaa ccaggagggt agttcctcta atgccgccat ctgtaactgc atccacctgg
      961 tttgcaagaa ccatcagacc cggctcggct atggatataa gaaatcccaa gacaagacct
     1021 gatacagcaa caattgaaac ttttccagtt gtggagatgg ttgcacccag aagtttgcca
     1081 aggggtgcaa tgccaatatc aatcccaatc aagaacagtg taagtcccac cactataagg
     1141 aatgccccaa ttataaaggt cagaagcatc gttccatcca gtggagtaag tgtgaagttt
     1201 agcattaaca caattatggt tatgggaagt actgataagg aaacttcctt gaatttgtca
     1261 aacaagaggt tcataggttc acatccagtg tagttatagt aaaaaaagtc attcaaacaa
     1321 gagtataccc aaattattta tagaataaca tcaattagtt tctggggaga catacttaga
     1381 cccagtaaaa tcaaaaacgt aacatttgtt actgaaagta tggtatatat tagtatagaa
     1441 tgtattcaga tactaaggag gttaacatat atgaataaat actttgaccc aaaggagaag
     1501 gtatacgata taaccgaaag gtacccagag acccttgatg tgtttgctgc aaatggattt
     1561 gaacacctta agaacgaagc aatgcggaag gtcatgggaa agacaatcaa ccttgagacc
     1621 gcctgcaaga ccaagaaggt caaccttcag ctttttgagc agaaactggt ggatgtgatt
     1681 gaacagacca ggggatcagt tgatgcgacc ctaaaccagg ccagggaaaa tggtgatggt
     1741 gaagtcagca tccttggagt gcttccatgc cccgtaagaa tacccttgat ggaaagcttc
     1801 agcaagtggc tggaagacca tgaaaaggac cttgactaca ggatagacta tgagcttaag
     1861 gctgcaaaca ttggagtgga ttggattcga gatgagatgg agaaggcttc ttcccatgac
     1921 atagcagacc ttttcatgtc agcaggtttt gatcttttct tcgatgaaaa gcttatgggg
     1981 aaattcaagt cacagggagt attccaggac atgtcaggct ttgaaagtct caatacagat
     2041 tttgacaatg ataggatttc actgaaggat cccatgggac aatacaccac acttgccgtt
     2101 gtaccagcag tgttcctggt aaataccgag gagctggatg gaagggagat gcccctgtca
     2161 tgggaggacc ttcttagtgg tgagttcgaa aactcaattt cactgcccct aaatgacttc
     2221 gaccttttca atgcactact cttgagcatt cacaaggagt ttggagagga cgggataaga
     2281 aggcttggaa gggcacacat gagaagcatg actccggcgg agatggtgaa gtccaatatt
     2341 aggaatactt catcaggaat acctacagtt actgtaatgc cctatctctt tgccaaaatg
     2401 gttagggaag agagtcccct gaagcctgta tggccaaagg agggcgcagt cataagtcca
     2461 atattccttc tttcaaagga ggacaagagg gacaaggtca aacctcttgt ggactacttc
     2521 atgtcaaagg aagtaggtca gatgctggct accagtggaa agttcccttc cacccacccc
     2581 gaggtggaaa acggacttac agaagaccag ggcttcatgt ggattggttg ggactacatc
     2641 aacagtcatc ctgacctggg agccttgata agggaactga tggatatctt caatgaggaa
     2701 gcaggtgtaa aatagatgaa tctagtgata ttttctggac ctccctcatc ggggaagacc
     2761 agtattatac taaagacaat tgaagcaatg agaaaaagag accttcaggt gggggttgtt
     2821 aagttcgact gcctaagtac agatgatgac atactttatg aaaaggcggg aatacctgtc
     2881 aagaagggac tttcaggagc actttgccca gaccactact ttgcctcaaa cattgaggag
     2941 gtagtgcaat ggggaaggaa aacaggcctg gaccttctaa taacagaatc cgccggacta
     3001 tgcaacaggt gttcaccata catcaaggaa attaagggag tatgtgtaat agacaacctt
     3061 tctggcataa atacacccaa gaagatcggg ccaatgctaa aggctgcaga tattgttgta
     3121 ataacaaagg gggatattgt atcccaggcg gaaagggagg tctttgcttc aaaggtaaac
     3181 tcagtaaacc caaaggccat gaccatgcac ataaatggcc taacaggtca gggagctttt
     3241 gaactgagca ccttgctcta tgatgaggat cgaaggcttg aggcagtaac aggaatggag
     3301 cttcggtatc ccatgccatc ggcactttgt tcatactgtc ttggagaaac caggattggt
     3361 gaggctttcc agatgggaaa tgtgagaaag attgaactgg gtgatatgga tgaataagat
     3421 gacaatagac cagatacta
//
LOCUS       DASRQF010000031        10168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1344529, whole genome shotgun sequence.
ACCESSION   DASRQF010000031 DASRQF010000000
VERSION     DASRQF010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 10168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10168
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344529"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /locus_tag="VFD79_00875"
     CDS             <1..1172
                     /locus_tag="VFD79_00875"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZH92627.1"
                     /translation="MEEERIPRLQEFSNTTELNRIEWNDRKIGIVTSGASYVHAKEVF
                     GDTVSYLKVGFSWPLPDAKFKEFAAGVEKIYVIEENEPYMEKHIKAMGIECTGKEIFS
                     VCGEINPQIIREAILGEKPEDGYSMDVVAPSRPPALCAGCPHRGIFFEMSKHKNKINV
                     TSDIGCYTLGAMAPLNVGDTVICMGAGISAGIGFDKVNRIAGREKKVFGVVGDSTFFH
                     SGMTGLVDAVYNKSNMVTVILDNRITAMTGHQENPGTGKSISGVESPQIDLIALVKAI
                     GIKDENLRVIDPYDMDANKEAVKAAIAADEPFVIITKQPCALIKDVMRKRANLYSQVN
                     QDKCRKCKTCLKIGCPAISMKDNVVSIDTTMCNGCEVCVQVCPFDAIESFGKEVN"
     gene            1173..1742
                     /locus_tag="VFD79_00880"
     CDS             1173..1742
                     /locus_tag="VFD79_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HZH92628.1"
                     /translation="MTKSLLLVGVGGQGTILVSKILSEGLLEEGYDVKMAEIHGMSQR
                     GGSVTTQIRFGDKVYSPTVNKGEADVLVSFEKIEALRYIPMLKKSGTLIINEEEIWPL
                     PVLAGMEEYPEGVMEEIKEKLDNIVSVDARSIAEDIGEPRSANIVMLGLMVKALGIDK
                     IDWISKIKHFLPERFHEANIKAFEKGLSL"
     gene            2016..2339
                     /locus_tag="VFD79_00885"
     CDS             2016..2339
                     /locus_tag="VFD79_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92629.1"
                     /translation="MNELTQKIIELDKNTLNMKKKYEDLLRKKKFEGQDKVHELEQQL
                     LKTFQQEGEKAYQEALKQTDSTLDNIEKIRQEYENSIKNTDEVYARMKPSLVDALWED
                     IIAES"
     gene            2398..3804
                     /locus_tag="VFD79_00890"
     CDS             2398..3804
                     /locus_tag="VFD79_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92630.1"
                     /translation="MAAKKSTMINVIGKIQDLDRILNDIIKTNHVRPLSAIELIQQNN
                     FLLDINNENVDRLVDINFASSYKKNIQAEKTLDKARELASMLGFELENEVPYKGKGMD
                     VNEVSDELDSLKDKVIDPCRMLEALREEIDYLERFHARSFKEILKFETSIDELRDMRY
                     FDFKIGILTRDNRHKLKKDYENILAVVQYTGSARDGEVYLIIYPENHKQEISRVLRTL
                     SFKEIVIPKNYTGTPREILDEIENKKAGIKKEISNLEKVVEGLKIHHSEWIHHLAGQL
                     LNLVKLEETKEMTAVSQNYFYFSGAIENHRSIHKVRDMLEGYDEIFMMFKEIQDDDEE
                     GRLIQALKRSDRYISDEKSADAIKGKDHAEAFGLALYTMDKEIAALRRKHEGMLRKKK
                     LEGLNRVHELEQELKNSYLEEAERIYRDAVEQKQLASASGEDDKYAKALDDLDEEFDG
                     LKTDLLERLWDEILNSKE"
     gene            3814..4869
                     /locus_tag="VFD79_00895"
     CDS             3814..4869
                     /locus_tag="VFD79_00895"
                     /inference="COORDINATES: protein motif:HMM:NF014094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HZH92631.1"
                     /translation="MGNERRFSAINTKLRVMRSRFLKDDDYLTLMRKDSVAEQIKFLA
                     TSTVYGQVLDEVENHDDIQEVELSLERFQITQFEKIIKYFSDDYEKYIKSFILRYETE
                     DLKLYLRALARGDDLEKFSQKSVTRKKYYSFSYDKVKSSKTLDEFIDNLKDTRFYDVL
                     YPYRHEDYTKLMFYMEMNLDRLYFDTLYQSGQELNKVDTRAFYDVFGENIDILNIEWI
                     YRGLKFYNLSPEELINYTLPHGKLFSFKDLKALCYGDIENLKKVVSGSIYKELVNEEK
                     DLDLFMGIRVQRHLYNKFKNSFSQGKLDISIANAYLHLLEFEIKDIISILESKKYGLS
                     YYETKEYLVRDIKGSDY"
     gene            4869..6815
                     /locus_tag="VFD79_00900"
     CDS             4869..6815
                     /locus_tag="VFD79_00900"
                     /inference="COORDINATES: protein motif:HMM:NF013647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HZH92632.1"
                     /translation="MSIEKMTMMNIIGKIDMVDDVLKNLLKTRKVDLVSAVSQIERSN
                     FLFDVNDENVDMLVDLNYIHTFEKSEECEEMFRKGEELKAALDLDFQVDRIDEDMKLV
                     SKEELSDELDSICKEVHGPSTMIVALKKELAYLDDFHSNSFRELEEFPVKVEDLRNME
                     YFDFKMGILSRENLLKLKKNYDNILSVLLHTGSSAEGEVYFAFYPKKLESEITRILRS
                     LNFKEIILPKSYTGTPEEIRASIESQKERLHMEIEGQQKILDDIKAKYQDRIRSIMEQ
                     IWITTRLDDLKEKVAFSSNYFYLSGWVAQSEKEEISSSVAGHDDVLIKFKEEENLEPP
                     TKLKNNWFFRPFQWLVNLYGTPSYNELDPTPFVGLSYMLLFGMMFGDLGQGFVMVVAG
                     YFISKKMELFGGLLSRIGIASMIFGTLYGAVFGIEHWIPALWMRPFENINDILIVAIF
                     VGIFVLLVAYIYGIINAYKRKDLEEGVFGHAGVAGVILYLTLLVTVGGSLLGISIIPV
                     PLGIGIAVAALLAIVFRLPLTHLIEGKRPLHGGDVSGYYVESIFSLIETFLSMLSGTI
                     SFIRVGAFALTHVGLFLAFETIGHMMGSAMGNVIVLIIGNIFVLGLEGLIVFIQGMRL
                     QYYELFSRYYSGDGKEFNPVSLGK"
     gene            6844..7275
                     /locus_tag="VFD79_00905"
     CDS             6844..7275
                     /locus_tag="VFD79_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HZH92633.1"
                     /translation="MSFVLISTTILVLLTIGTGAFMMYKEMEGGKSKMKKILRTNLFV
                     FLPLLIVAVIVMVPQGISAAETEAGLLSSSAGLGFISAALATGLATLGAGYAVAVVGA
                     AALGAVSEDATLLGKTLIFVGLAEGIAIYGLIVSIMILGRL"
     gene            7278..7583
                     /locus_tag="VFD79_00910"
     CDS             7278..7583
                     /locus_tag="VFD79_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004832024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HZH92634.1"
                     /translation="MKSILISDNKDTIIGMRLASIDGVLVKTREETIERLNEAAMDDE
                     VGIIIVTENIFDEITDRILELKRSGSKKLVVTIPDRSGLKDKNFIMRHIKESIGIKI"
     gene            7595..8224
                     /locus_tag="VFD79_00915"
     CDS             7595..8224
                     /locus_tag="VFD79_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HZH92635.1"
                     /translation="MITVEEKLEIFHRIVYRDEEEKYLKALKELEEYNQQLLENKKEE
                     MKRQRDEIIRRKITQAKVERNELKSEGLEDTKAKLRQKRKELETDLVDSIVERANEFV
                     KSKGYGEYLCRNLDKYLVEFEEDEVVLILRDEDRLISESCLMKLKDQTGKTFIVETLP
                     DEKIGGFMMSNKDRTFNIDHTLKTLIQEKEYMIGKRLNDALGEVGEING"
     gene            8217..9989
                     /locus_tag="VFD79_00920"
     CDS             8217..9989
                     /locus_tag="VFD79_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HZH92636.1"
                     /translation="MASLMGEIQMINGPVILGKNMTGFKMREMVTVGEKKLIGEIIAI
                     DGDQGTVQVYEETEGLRRGEQIVSTGKPLSLKLGPGMIGNMFDGIQRPLKKIKEDHGS
                     FIPEGIGLISLDEEKLWEVEFQVEEGDYVKQGQIFGTIQESPLITHRLLIPVGVEGKV
                     TFRAVPGEYNINKVLLKILDLKGEEHEVKMYQEWPVRLQRPVAERLQIDKLLITGQRV
                     LDIFFPIAKGGTAAIPGGFGTGKTMTQHQLAKWSDADIIVYVGCGERGNEMTEVLEDF
                     PELIDPKSNRPIMERTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYNVAIMADST
                     SRWAEALREISGRLEEMPAEEGYPAYLPSRLAQFYERAGYVKTLQGDTGSITVIGAVS
                     PAGGDFSEPVTENTKRFVNVFLGLDRELAYSRHYPAINWLNSYSGYSEMIKRYYEKEL
                     GESIEDIRKKMLNLLFEESKLLEIVLLVGEDVLPDDQRLILEIARVLKIGFLQQNAFH
                     KDDTYVPLRKQIDMLKTIELLYDRGLQSVKKGIPISVVRNIELYEDIIKMKYTITNDD
                     IEKIQGLNARINEFYNQLESEYSA"
BASE COUNT         3381 a         1710 c         2593 g         2484 t
ORIGIN      
        1 agatggaaga ggaaaggatt ccaagacttc aggaattctc aaacactaca gaactaaaca
       61 ggatagaatg gaatgaccga aaaattggaa tagtgacatc cggagcaagc tatgttcatg
      121 caaaggaagt cttcggtgac actgtttcct atctcaaggt tgggttcagc tggcctctgc
      181 cagatgcaaa gttcaaggag tttgcagctg gagttgaaaa aatctatgta atcgaagaaa
      241 acgaaccata tatggagaag catataaagg caatgggaat tgagtgtact ggaaaggaaa
      301 tattctcagt atgtggcgag ataaacccac agatcattag ggaagcaatt cttggagaaa
      361 agccagagga tggatacagc atggatgttg tggcgccctc aagaccacct gccctttgtg
      421 caggctgtcc tcacagggga attttctttg agatgtccaa gcacaagaac aagataaatg
      481 taacctctga cataggttgc tacacccttg gagcaatggc accgcttaat gttggtgaca
      541 ctgtaatctg catgggagct ggaataagtg caggaatagg ctttgacaag gtcaacagga
      601 ttgccggaag ggaaaagaag gtatttggag tggttggaga ctcaaccttc ttccattcag
      661 gaatgactgg acttgtggat gctgtataca acaagagcaa catggtgaca gtgattcttg
      721 acaatagaat cactgccatg acgggacacc aggaaaaccc gggaaccgga aaatcaatct
      781 caggtgtgga atctccacag attgacctta ttgcacttgt aaaggcaata ggaataaagg
      841 atgaaaacct tagggtcatc gatccatatg acatggacgc aaacaaggag gctgtaaaag
      901 ctgcaattgc tgcagatgag ccatttgtaa tcataacaaa gcagccttgc gcactgatca
      961 aggatgtaat gagaaaaaga gcaaacctat attcccaggt aaatcaagac aagtgtagaa
     1021 agtgtaaaac ctgcctgaaa atcggttgtc cagccatatc catgaaggat aatgtagttt
     1081 caatcgacac tacaatgtgc aacggctgtg aggtctgcgt tcaggtatgt ccgtttgatg
     1141 cgattgaatc atttgggaaa gaggtgaatt agatgactaa gagcttattg ttggtaggag
     1201 tgggaggaca gggtacgatc cttgtttcaa agatattgtc cgaaggcctt ctggaggaag
     1261 gctatgatgt taagatggct gaaatccacg gtatgtccca gcgtggaggt agtgttacaa
     1321 ctcagataag atttggtgac aaggtctatt caccaactgt aaacaagggt gaggccgacg
     1381 tacttgtatc ctttgaaaaa attgaggcat tgagatatat tccaatgctg aagaaaagcg
     1441 gcaccttgat cattaatgag gaggaaatct ggccattgcc tgtattggca ggtatggaag
     1501 agtatcctga aggtgttatg gaggaaatca aggaaaagct tgataatatt gtctccgtag
     1561 atgccagaag tattgctgag gacattggtg agcccagatc tgccaacatt gttatgcttg
     1621 ggcttatggt aaaggctctt gggattgata agattgactg gataagcaag ataaaacact
     1681 tccttccaga gcggttccat gaagcgaata ttaaggcctt tgaaaaaggt ttatcactat
     1741 aggatgaaaa gtgccacttt tatgtggcgc ttttttgtac atatggctta gttactggga
     1801 cctaagtcct ggaggaaata ttaagaattc gtaacaataa aggaaaaggg agatatttgc
     1861 agaaaaaaag agtaaaatga agataacaac aaatgttaga caattaacaa actaaaaccg
     1921 cgaaaattgg gccttttagg gcagattagc aacatattga aattgtttaa tattttatat
     1981 caagtataat agataggggc gaggagtgat aaaaaatgaa cgaacttaca caaaaaatca
     2041 ttgaacttga caagaatacc ctcaatatga agaagaagta cgaggatctt ctcagaaaga
     2101 aaaagtttga gggtcaggac aaggttcatg aactggagca gcagcttctg aaaacctttc
     2161 agcaagaagg ggaaaaagcc taccaggaag cgctgaaaca gacagatagt actttggaca
     2221 acattgagaa aatcagacag gaatacgaaa acagcattaa gaacactgat gaggtatatg
     2281 caaggatgaa gccaagcctt gtggacgctt tatgggagga tataatcgca gaatcctagg
     2341 taagaccgct tgcgaaacat atgaacccgg cagtaaaaca aaaggaagtg ataagacatg
     2401 gcggcaaaga agagtacaat gattaatgtc attggaaaaa tccaggacct ggacaggatt
     2461 ttgaatgaca taattaagac aaaccatgtt agaccattat cagcaatcga gctgatacaa
     2521 cagaacaact ttcttcttga tatcaacaat gagaatgtgg ataggcttgt agacatcaac
     2581 tttgcatcat cctacaagaa gaacatccaa gcggaaaaaa ctttggacaa ggctagggaa
     2641 ctggcatcga tgttaggttt tgagcttgag aatgaagtcc cgtacaaggg aaaagggatg
     2701 gatgtcaatg aagtgtccga tgagctggat agcttgaagg ataaggtcat agacccttgc
     2761 aggatgcttg aggccctgag ggaggaaatt gactatcttg aaaggttcca tgcaaggagt
     2821 ttcaaggaaa tacttaaatt tgagacatcc atagatgaat tgagggatat gaggtatttt
     2881 gacttcaaga tagggatact gaccagggac aataggcaca agctcaagaa ggactatgag
     2941 aatattctcg ctgttgtaca gtatactgga agtgccagag atggagaggt atacctgata
     3001 atctatccgg agaatcacaa gcaggaaatc tcaagggtcc taagaaccct gagctttaag
     3061 gaaattgtaa tacccaagaa ttacaccggt acaccaaggg agattctcga tgagattgag
     3121 aataagaagg caggcatcaa gaaggagatc tcgaaccttg aaaaggtggt tgaaggcttg
     3181 aagatacacc atagtgaatg gatacaccat ttggcaggtc agctcctgaa cctggtcaag
     3241 ctggaggaga ccaaggagat gacagcggta tcccagaatt acttctactt ttctggggcg
     3301 attgagaacc acaggagtat tcacaaggta agggacatgc tggaaggata cgatgaaatc
     3361 tttatgatgt tcaaggaaat tcaggatgat gatgaggaag gaaggcttat tcaagccctc
     3421 aagaggagcg acaggtatat ttctgatgag aagtcagctg atgcaatcaa gggaaaggac
     3481 cacgcagagg catttgggtt agccctttac accatggata aggaaatcgc tgccctaagg
     3541 agaaagcatg aaggaatgct tcggaagaaa aaactggaag gtcttaacag agtccatgag
     3601 ctggagcaag aactgaaaaa ctcatacctg gaggaagcag agaggatata ccgggatgct
     3661 gttgagcaga aacaactggc atcagctagc ggtgaggatg acaaatatgc caaggcattg
     3721 gacgatctgg atgaggaatt cgatggtttg aagacagatc tactggagag actgtgggac
     3781 gagattttaa atagcaaaga atagagggat agaatgggaa atgaaagacg tttttcagca
     3841 atcaatacaa aactaagagt catgcggtcc cgtttcctca aggatgatga ttacttaacc
     3901 ctcatgagaa aggattccgt cgctgaacag ataaagtttc tggccacaag tacggtatac
     3961 ggacaggttc tcgatgaggt ggagaaccat gacgatatac aggaggttga actaagtctg
     4021 gaaaggttcc agattaccca gtttgagaag atcatcaagt atttctccga cgattatgag
     4081 aagtacataa agtcctttat cctaagatac gagacagaag acttgaaact ttacttaaga
     4141 gcacttgcca gaggggatga cctggaaaag ttcagtcaga agagcgtaac ccgaaagaag
     4201 tattacagct tctcatacga caaggtgaag tcatcgaaga ctctggacga attcatcgac
     4261 aacttgaagg acaccaggtt ctatgatgta ctctacccat acaggcatga ggattacacc
     4321 aagttgatgt tctatatgga aatgaatctg gacagacttt attttgatac cttgtatcaa
     4381 agcggacagg agctcaacaa ggtggacaca agggcattct atgatgtctt tggtgaaaat
     4441 attgacatac tgaatatcga atggatatac agggggttga agttttacaa cctgtcccca
     4501 gaggaattga taaactacac tttgcctcac gggaaactgt tcagtttcaa agatttgaag
     4561 gcactatgct atggagacat agagaacctt aaaaaagttg tttccggtag tatctacaag
     4621 gaattggtca atgaggaaaa ggatttggac ctttttatgg gaataagggt tcagagacat
     4681 ttgtacaata agtttaaaaa ttcattcagc cagggaaaac ttgatatctc aatagcaaac
     4741 gcttatctcc acctactgga gtttgagatc aaggatatta tttcaatact ggaatcaaaa
     4801 aaatatggat tatcctacta cgagaccaaa gagtatctag tcagggatat aaaaggaagt
     4861 gattattaat gtccattgaa aagatgacca tgatgaacat cattggaaag attgacatgg
     4921 tggatgatgt tctaaagaat cttctcaaga caagaaaagt ggacctggtg tcagccgtat
     4981 ctcagattga aaggagtaat ttcctgttcg atgttaatga tgagaacgta gacatgcttg
     5041 ttgacctgaa ctacatccac acattcgaaa agagtgagga atgtgaggaa atgttcagaa
     5101 agggagagga gcttaaggca gctcttgatc tggactttca ggttgacagg atagatgagg
     5161 acatgaagct tgtcagcaag gaagaattgt ctgatgaact tgatagcatt tgcaaggaag
     5221 ttcatgggcc aagcaccatg atagtggctt tgaagaagga gctggcatac ctggatgact
     5281 tccattcaaa cagcttcagg gagttggagg aattcccagt aaaggttgag gacttgagaa
     5341 acatggagta ctttgacttc aagatgggga tactaagcag ggagaacctt ctaaaactga
     5401 aaaagaacta tgacaacata ctgtcagtac tcctacatac aggatccagt gcggaagggg
     5461 aagtgtattt tgctttctac cccaagaagt tggaatctga gataacgaga atattaaggt
     5521 ctcttaactt caaggaaatc atacttccaa agtcatacac tggaacgcca gaggaaatta
     5581 gagcaagcat agaaagccag aaggaaagac tccacatgga gattgagggg cagcagaaga
     5641 tactggatga catcaaggcc aagtatcagg acaggataag gtccataatg gagcaaatat
     5701 ggataactac aagattggat gacctcaagg agaaggtagc cttttccagc aattacttct
     5761 acctatcagg ctgggttgca caatctgaga aggaagaaat cagcagctca gttgctggtc
     5821 atgatgatgt gctaataaag tttaaggaag aggagaacct cgagccacct acaaagctta
     5881 agaacaactg gttctttaga ccattccagt ggcttgtgaa cctttatgga actccaagct
     5941 acaatgagct tgacccaact ccatttgtgg gactgtccta catgctattg tttggcatga
     6001 tgtttggtga cctcggacag ggatttgtaa tggttgttgc aggctacttc atcagcaaga
     6061 aaatggaact ttttggaggc ttgcttagca gaataggaat tgcctcaatg atatttggaa
     6121 ccctttacgg ggcagtgttt ggtatagagc attggatacc tgcactttgg atgagacctt
     6181 ttgagaacat taacgacatt ctgattgtag ccatatttgt aggtattttt gtacttctgg
     6241 ttgcgtatat ctacggaatc atcaatgcct acaagagaaa agaccttgaa gagggagtat
     6301 ttggtcatgc aggtgtagct ggagttattc tctatttgac cctgcttgtg acagtcggcg
     6361 gcagcctatt gggaatcagc ataataccgg ttccattagg cataggcatt gcagttgcag
     6421 cactattggc aattgttttc agattgcctc taacccactt gatagaaggg aaaagaccgc
     6481 ttcatggcgg agacgtgagc ggatactatg ttgaatccat attctcactg atagaaacct
     6541 tcttgagtat gttgagtggt accatatcct ttataagggt aggagcattt gctctgacac
     6601 acgtcggatt gttccttgca tttgaaacca taggtcatat gatgggttct gcaatgggga
     6661 atgtaattgt tcttatcatt ggtaatatat ttgttttggg actggagggt ctgatcgtat
     6721 tcattcaggg catgagactt cagtactacg agcttttcag caggtattac agtggagatg
     6781 gaaaagaatt caaccctgta agtctgggca agtaattatt atttaattct aggaggagat
     6841 tatatgtcat ttgtattgat atcaacaaca atattggttc tacttactat aggtacggga
     6901 gcatttatga tgtacaagga aatggaaggc ggaaagagca agatgaagaa aattctaagg
     6961 acaaatcttt ttgtattcct tccactattg atcgttgcag tgattgttat ggtaccgcag
     7021 ggaatcagcg cggcggaaac tgaagcaggt cttctttcat catcagctgg acttggtttc
     7081 atatctgcag ctctggcaac tggtttggca acacttggag caggttatgc cgttgcagta
     7141 gtaggggcag ctgcccttgg agcggtttca gaggatgcaa cacttcttgg taaaaccctt
     7201 atattcgttg gtcttgcaga gggtatcgct atctatggtc tgattgtttc aatcatgatt
     7261 ctaggtaggt tatagatatg aagtccatcc tgatcagtga taacaaggat acaataattg
     7321 gaatgagact ggccagcata gatggggtat tggtaaaaac cagggaagag accattgaaa
     7381 gactaaacga agctgcgatg gatgatgaag taggcataat tatagtaacc gagaatattt
     7441 ttgatgagat aaccgatagg attctggaac ttaagagaag tggaagcaag aagcttgtag
     7501 ttactattcc ggatagaagc ggcctaaagg ataaaaactt tattatgagg catattaaag
     7561 aatccatagg cattaaaatt taggtggtga acttatgata acagttgaag aaaaacttga
     7621 gattttccat agaattgttt acagggatga agaagaaaag tatcttaagg ctcttaaaga
     7681 actggaggag tacaatcagc agcttcttga aaacaaaaaa gaggaaatga aaagacagcg
     7741 tgatgaaata atccgaagga agataactca ggcaaaggtt gaaaggaacg aactgaaatc
     7801 agaaggcctt gaggatacca aggcaaagct cagacagaag agaaaagagc tggaaactga
     7861 tttggtggat tcaatagtgg aaagggcaaa tgagtttgtc aaatccaagg gatatggaga
     7921 atatctgtgc agaaacctgg ataagtatct ggttgaattt gaggaggacg aagtggtttt
     7981 gatcctaagg gacgaggaca ggctaatatc agaaagctgc cttatgaagc ttaaggacca
     8041 gaccggcaag accttcattg tggaaaccct gccagatgaa aagataggtg gctttatgat
     8101 gagtaacaag gatagaacat tcaacatcga ccatacattg aagaccttga ttcaggagaa
     8161 ggagtacatg ataggtaaaa gactaaacga tgctcttgga gaggtaggtg aaatcaatgg
     8221 ctagtttgat gggagaaatt cagatgataa acggtcctgt aattctggga aagaatatga
     8281 caggattcaa gatgagagag atggttacag ttggcgagaa aaagctcata ggtgagatta
     8341 ttgccattga cggagaccag ggaacagtcc aggtatacga ggaaacagag ggacttagaa
     8401 gaggcgagca gatagtatca acaggcaagc ccctatccct aaagctggga ccaggaatga
     8461 tcgggaacat gttcgacggt atccaaagac ccctaaagaa gatcaaggaa gaccacggaa
     8521 gctttattcc agagggcata ggacttatat ccctggatga ggaaaagctt tgggaggtgg
     8581 aattccaggt tgaggaaggg gactatgtca agcaaggtca gattttcggg accatacagg
     8641 aaagcccctt gataacacac agactattga taccagtggg tgtggaagga aaggttacct
     8701 tcagggcagt acccggtgaa tacaatataa acaaggtact tctaaagata ctggacctga
     8761 aaggtgagga acatgaggta aagatgtacc aggagtggcc tgtaagacta cagagacctg
     8821 ttgctgaaag gcttcagata gacaagctat tgattacagg acaaagggtt ctggatatat
     8881 tcttcccaat agccaaggga ggaactgcag caattcctgg tggtttcggt acaggaaaga
     8941 caatgaccca gcaccagctg gccaagtggt cagatgctga tataatagta tacgttggtt
     9001 gcggagagag gggtaacgag atgacggagg ttctagagga cttccctgaa ctgatcgacc
     9061 ccaagtccaa cagaccaata atggaaagaa cagtacttat tgcaaacaca tcgaacatgc
     9121 ccgttgcggc cagagaggca tccatctaca ctggaataac aatggccgag tactttaggg
     9181 acatgggcta taacgtggcc ataatggccg actccacatc aagatgggcg gaggccttaa
     9241 gagagatttc aggccgtttg gaggagatgc ctgcagagga gggatatcca gcctaccttc
     9301 catcaagatt ggctcagttc tatgaaaggg cgggatatgt caagacccta cagggagaca
     9361 ccggttcaat cacggtaatc ggggcagtat cacctgcggg aggagacttt tccgagccag
     9421 taacagagaa tacaaagagg ttcgtaaatg tattccttgg actggacagg gaattggcat
     9481 actcaagaca ttaccctgca ataaactggc tgaattcata tagtggatac agtgaaatga
     9541 taaagagata ctatgaaaag gagctgggag agagtataga ggatatcagg aagaagatgc
     9601 tcaacctctt gtttgaggaa agcaaacttc ttgagatagt acttcttgtt ggtgaggatg
     9661 tacttcctga tgaccagagg cttattctcg aaatagcaag agttcttaag attggtttcc
     9721 ttcagcagaa tgcattccac aaggacgata cctatgttcc tttaagaaag caaatagata
     9781 tgcttaagac catagaattg ctctatgaca ggggtcttca gtcagtcaag aagggaatac
     9841 caatatcagt tgtgagaaac atagagttgt acgaagacat catcaagatg aaatacacca
     9901 ttaccaatga cgacattgaa aaaatacaag gactgaatgc aaggatcaac gagttctaca
     9961 accagttgga atctgagtat tcagcatagt gggtgattag atgaaaagag aatatttaaa
    10021 attagataga gtacaaggag ctctgataga gctctcaaaa gttcattcag tgggatatgg
    10081 tgagatagcc cagattgagg acagattggg caactcaatg gtaggtcgtg taatcaagat
    10141 tgatggggat aatgttgtgg ttcaggta
//
LOCUS       DASRQF010000032         5667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1343663, whole genome shotgun sequence.
ACCESSION   DASRQF010000032 DASRQF010000000
VERSION     DASRQF010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343663"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1115)
                     /gene="rbsC"
                     /locus_tag="VFD79_00925"
     CDS             complement(147..1115)
                     /gene="rbsC"
                     /locus_tag="VFD79_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HZH92637.1"
                     /translation="MGKFSLKGRNWKQFLSDNKPVVVLLILVVTMSFLSDRFLTWSNI
                     ITVLRQTSINAVIATGMTFAILIGGIDLSVGSVLAISGALAASLVASGMNIVLVILIT
                     LLIGLIIGLLNGLLISKGRLQPFIATLGTMTLLRGLTLVYTQGRPISIFGSDTSAMFS
                     KIGTGYFLGIPIPVYIMLVVFFISWYTLNHLKIGRYTYALGSNEEATMYSGIKTDSIK
                     LFVYAVAGVLAALAGIIVTARLGSAQPTAGSGYELDAIAAVVIGGTSMAGGIGTIFGT
                     AIGAMIIGILNNALNMLQVSSYYQDVAKGVVILIAVLLDRKQRKSR"
     gene            complement(1116..2612)
                     /locus_tag="VFD79_00930"
     CDS             complement(1116..2612)
                     /locus_tag="VFD79_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZH92638.1"
                     /translation="MEEKVVSMENIIKEFSGVRVLDNVDFNIYKGKVMALVGENGAGK
                     STLMKILTGVYVRTSGKVVLNGEPVEFNTTKESQDKGIAFIHQELNLVRDLSIGENIF
                     LGREPVGPAGKIRWKKLYDDSREWLTKLGLEEDPRELVRNLSVGKQQMIEIAKALSLD
                     AQVIIMDEPTGALTLSETDRLFKVVKELRGSGHSIVYISHRLNEIFEICDHVTVLRDG
                     TFVGEEPIENLDEDRIIEMMVGRKLSEQYPRVETEIGDVVLEVKNLSNRFVEDVSFQL
                     RKGEILGVAGLVGAGRTELARTIYGVYQPESGEILVNGSPVSIKSPKDALKNGIAYLS
                     EDRKENGIVLGMDLVENVTLASLNNYVGRIGSIDKSKERESTDGYIKSMSIKAASRNQ
                     LLQFLSGGNQQKVALAKNLDTNPGILLLDEPTRGVDVGAKKEIYELINKYKQEGMSIL
                     MVSSEIPEILGISDRVMIMHEGRVRGIIDIAEADQEKIMTYAVGKEVS"
     gene            complement(2636..3043)
                     /gene="rbsD"
                     /locus_tag="VFD79_00935"
     CDS             complement(2636..3043)
                     /gene="rbsD"
                     /locus_tag="VFD79_00935"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HZH92639.1"
                     /translation="MKKGFLLNSEVSSVISHMGHMDELTVGDAGLPIPSETYRIDLSV
                     FEGVPSFLDVLRAVLSEQKVEGIILAEEIKEASPEMHSKILRLVEEFEDEFTVTYLTH
                     DDFKTRTKESKAVVRTGEFTPYANVILISGVVF"
     gene            complement(3046..3981)
                     /gene="rbsK"
                     /locus_tag="VFD79_00940"
     CDS             complement(3046..3981)
                     /gene="rbsK"
                     /locus_tag="VFD79_00940"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HZH92640.1"
                     /translation="MVIVVGSLNMDLVIRAPKIPRPGETVLGSDFRQVPGGKGANQAD
                     AAAKLGAETAILGAVGRDSMGSTLVQALKMDGVKVDMVLEKEDLPTGVAAIVVEDSGN
                     NAITVAPGANSGLTPHDISSMEGIFKGATVMLVQLETPLDTVKRALQLARENNVKTIL
                     NPAPAMELDDEIFSLVDIMTPNETELEILSGMDTSDLDKVESAGQYLLKKGVSRLIVT
                     LGSQGSMDIVQDSATTYPAYKVKAVDTTAAGDSFNAALAVSIANGESMEDSIRFATKV
                     GAMTVTKHGAQTSLPLVGQVEDFEEWYEKMKLEER"
     gene            complement(3996..5003)
                     /locus_tag="VFD79_00945"
     CDS             complement(3996..5003)
                     /locus_tag="VFD79_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZH92641.1"
                     /translation="MKKRHSTIKDIAKAAGVSTATVSMIMNKKDKNISQPTREKVLKI
                     AKEINYIPNSMARSLVTSQTKTLGLVLPDIVNPFFPEIARGAEDRAREAGYSIIICNT
                     DDNVDQENKYIQMLSEKMVDGIILTHSANRSDEMIGLENSRVPIILIDRDYNSPNVLG
                     KVLVDNSRASRDGVKYLIDKGYRRIAYIAGPLNTGTARDRLHGYEIALAEEGIAYDEA
                     LVRVGEYKSKWGYDAAEDLLDSQIDFQAIFCGNDAIAIGAMKKLRERGIKVPEDIAIM
                     GFDDIYIASMVDPELTTIKQPNYSMGYEAVNMMIDSIQGKRIDSRNLILETELVVRKS
                     S"
     gene            5177..5437
                     /locus_tag="VFD79_00950"
     CDS             5177..5437
                     /locus_tag="VFD79_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92642.1"
                     /translation="MGDRRYEYGNNDPVSVSEWIITTIVMAIPLVNIVMPFVWAFGSG
                     TKTSKANFFKAQIVMVLIGLVLYLLVGGMLFSYIRMPSQSLF"
     gene            5460..>5667
                     /locus_tag="VFD79_00955"
     CDS             5460..>5667
                     /locus_tag="VFD79_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92643.1"
                     /translation="MNKFHYNGDRRKNMINIVAFMGPSGSGKTTLAKYINIRKVTTYT
                     TRPSREGERDGIDYYFMKEEELLRL"
BASE COUNT         1389 a         1425 c         1185 g         1668 t
ORIGIN      
        1 tcctcctgta gtacatcctg caagccccac tgccaacaca agggccagcg ccagtgcaaa
       61 ataccttttt ttgttcatca attttttctc cccctttttt gtttatcgct ttttatctat
      121 ttcaacccac atgggttaaa accaggttaa cgtgacttac gctgcttcct gtcaagaagt
      181 acagctatca gtattactac acccttggca acatcctggt agtaggatga aacctgaagc
      241 atgttaaggg cgttgttaag tattccaatt atcatggcac caattgcagt tccaaatatt
      301 gttccaatcc caccggccat acttgttcca ccgattacaa ctgcggcaat ggcgtcaagc
      361 tcatagccgg atccggcagt tggctgggct gaaccaagcc ttgcagttac gatgattcct
      421 gcaagtgctg ccagtactcc tgccacagca taaacaaaca gctttatact gtcggtcttt
      481 atacctgaat acattgttgc ctcctcgttt gaacccaggg cataggtgta tctgccgatc
      541 ttcaggtggt ttagtgtata ccaggaaata aagaagacca caagcattat atatactggt
      601 attgggattc ccaggaaata gcccgtaccg atttttgaga acattgccga ggtgtctgaa
      661 ccaaagatgc ttatgggcct tccctgggta taaaccaatg ttaggcctct taagagggtc
      721 attgtaccca gtgtggcgat aaaaggctga agcctgccct tgcttataag gagtccgttc
      781 agtagaccaa ttatcagacc aatgagcagg gtgatgagta tgacaagcac tatgttcatt
      841 ccacttgcaa caagacttgc tgcaagtgca ccagatattg caagaacgct gcctacggaa
      901 aggtcaattc ctccaattag gattgcgaag gtcatacctg ttgcaataac tgcgtttatt
      961 gaggtctgtc tcaatacggt tatgatgttt gaccaggtca gaaacctgtc gctaagaaag
     1021 gacatggtca caaccagtat cagaagcacc acaaccggct tgttgtctga taaaaactgc
     1081 ttccagttcc tccccttcaa tgaaaactta cccatttagc tcacttcctt tcctacggca
     1141 taggtcatga ttttttcctg gtcagcttca gcgatatcaa tgatacccct aacccttccc
     1201 tcgtgcatga tcattaccct atcgcttatt cccaatattt caggtatctc agaagaaacc
     1261 ataagtatgc tcatgccttc ctgcttgtat ttgttaatga gttcatagat ttcctttttg
     1321 gcacctacat ccacacctct ggttggttca tccaagagta ggataccagg atttgtatcc
     1381 aggttttttg caagtgcaac cttttgctga tttccaccac ttaggaactg aagcagttga
     1441 tttctactgg cggcctttat tgacatgctc ttgatatagc cgtcggtgga ctcccgttcc
     1501 tttgatttgt caatgctgcc tatcctaccc acataattgt taagggaggc gagggttaca
     1561 ttctcaacta ggtccattcc aagtacaata ccattttcct tcctgtcctc tgaaaggtag
     1621 gctatcccgt ttttcaaagc atccttggga ctcttaattg aaacaggaga cccatttaca
     1681 aggatctctc cagattcagg ctggtagacc ccataaatgg tcctggctag ctctgtcctt
     1741 cctgcaccta ccaggccggc aactccaagg atttctccct tcctgagctg gaatgaaaca
     1801 tcctccacaa atctgttgga aaggtttttc acctctagga ccacatctcc aatttctgtt
     1861 tccacccttg ggtactgttc gcttagcttt cttcccacca tcatctcaat gatcctgtct
     1921 tcatcaaggt tttcaatggg ctcttctccc acaaaggttc cgtccctaag gacggtgaca
     1981 tggtcacaga tttcgaaaat ttcatttagc ctgtgtgaaa tgtatacaat gctgtgtcct
     2041 gaacccctta actccttgac aaccttgaac aacctatctg tttcagacag ggtaagtgca
     2101 ccggtgggct cgtccatgat tataacctgg gcatccaggg atagtgcctt tgcaatttca
     2161 atcatctgct gctttcctac actaaggttt ctgaccagct ccctggggtc ctcctcaagg
     2221 ccaagctttg tcaaccattc ccttgagtca tcataaagct ttttccacct tatcttaccg
     2281 gctgggccca cgggctccct acccaggaat atgttctcgc caatggatag gtccctgacc
     2341 aggtttagct cctgatggat aaatgctatc cccttatcct gggattcctt tgtggtgttg
     2401 aactctacag gttctccatt gagaacaacc ttgcctgaag tccttacata aacaccagta
     2461 agtattttca tcaaggtgga ctttccggct ccgttctcgc ccacaagcgc catgacctta
     2521 cccttgtaga tgttgaaatc aacattgtcc agaaccctta cgccggagaa ctcctttatg
     2581 atgttttcca tcgatacaac tttttcttcc atggagattt cctcctcata gattattaga
     2641 atacaactcc tgatatcagg atcacatttg cgtatggtgt gaattcaccg gtccttacaa
     2701 ctgccttgga ttctttagtt ctggtcttga aatcatcgtg tgttaggtag gtgactgtaa
     2761 actcatcctc aaactcctca acaagcctaa ggatttttga gtgcatctct gggcttgctt
     2821 ccttgatctc ttctgccagg attattcctt ccaccttttg ttccgacagt acggccctta
     2881 ggacatccag aaatgagggg acaccttcaa atactgaaag gtcaatcctg taggtttcag
     2941 agggaatggg aagacctgca tctccaactg tgagctcatc catgtggccc atgtgtgata
     3001 tcacagagga tacctctgaa ttgagcaaaa agcctttttt cataatcatc tctcctcaag
     3061 cttcattttt tcataccatt cttcaaaatc ctccacctgg ccaacaaggg gcagggaggt
     3121 ctgggccccg tgctttgtta ccgtcatggc tccaaccttg gtggcaaacc ttattgaatc
     3181 ctccatggat tctccattgg ctatggatac tgccagtgcc gcattgaagc tgtcacctgc
     3241 agctgttgta tcaacggcct tgaccttata tgcagggtag gttgtggctg aatcctgaac
     3301 aatgtccatg cttccctggc tgccaagggt tactataagc ctggagacgc cctttttaag
     3361 aaggtattgt ccggcacttt caaccttgtc aaggtcgctg gtatccatgc cgctcaatat
     3421 ctcaagctca gtctcgtttg gagtcatgat atccacaagg gagaatatct catcatcaag
     3481 ctccattgcg ggagctggat tgagaatggt ctttacattg ttttctcttg ccagttgaag
     3541 agcccttttc acagtatcca aaggagtttc cagctgcacc agcattactg tggccccttt
     3601 aaatatccct tccatactgg aaatgtcatg gggagtaagg ccagaatttg ctccgggagc
     3661 aactgttatg gcattgttgc ctgaatcctc aacaacaatt gcagcaaccc ctgtgggaag
     3721 gtcttccttt tccagaacca tgtccacctt tacgccatcc attttcagtg cctggaccag
     3781 ggtgcttccc atggaatccc tcccaacagc tccaaggatg gctgtctctg caccaagctt
     3841 tgcagctgca tcagcctggt ttgccccttt tccacctggt acctgtctga aatccgatcc
     3901 aaggaccgtt tctccaggtc tgggtatttt tggagccctt atcacaaggt ccatattgag
     3961 actgccaaca actataacca taatcaatgc tcctttcagc tggattttct taccacaagc
     4021 tccgtttcca ggataaggtt cctactgtca atcctcttgc cctgtatgga gtctatcatc
     4081 atattgaccg cttcataacc catggaatag tttggctgct tgattgtcgt aagctccggg
     4141 tccaccatgg aggctatgta tatatcgtca aagcccatta ttgcaatatc ctccgggacc
     4201 tttatccccc tttcccggag cttcttcatg gccccgattg caattgcatc gttaccacag
     4261 aagatggcct ggaagtcaat ctggctgtcc agaaggtcct ctgctgcatc atagccccac
     4321 ttggacttgt attcaccaac cctgaccagg gcctcgtcat aagcaattcc ttcctcggca
     4381 agggcaattt cgtaaccatg gagcctgtct ctagcagtac ctgtattgag aggacctgca
     4441 atgtatgcaa tcctcctgta gcccttgtct ataaggtact tcaccccgtc cctggacgcc
     4501 cttgaattat ccaccagaac cttgcccagg acattggggg aattgtaatc acggtcgatc
     4561 agtataattg ggacacggct gttttcaagg cctatcattt catcacttct attggcggag
     4621 tgggtcagga ttatcccatc caccattttt tctgatagca tctgaatgta cttgttttcc
     4681 tggtccacat tgtcatccgt gttgcatatt attatgctgt agcctgcttc cctggccctg
     4741 tcttcggccc cccttgcaat ctcggggaag aaggggttga caatgtctgg aaggaccagc
     4801 cccagggtct tggtctggct tgtaacaagg cttcttgcca tggaatttgg tatatagttg
     4861 atttccttgg cgatcttgag aaccttctcc cgtgtaggct gtgaaatgtt cttgtccttt
     4921 ttattcatta tcattgaaac cgtggcagtt gagacaccag ccgccttggc tatatcttta
     4981 attgtggagt gtcgtttttt cattgaaaga tcccaccttg gttaaacgtt taactacacc
     5041 tatattatac caccaaaagt caagatggaa acatcaatca ggatatttta caagccggca
     5101 atattggaag tggcctccag tagtgatata atcaagctag gagagatatc taaatttaga
     5161 tgaaaggaga attcctttgg gagataggag atatgaatac ggcaacaatg atcctgtaag
     5221 cgtttccgag tggataatta caacaatagt tatggccata cccctggtaa acatagtgat
     5281 gccctttgtc tgggcatttg gaagtggaac aaagacaagc aaggccaact ttttcaaggc
     5341 acagattgtt atggtcctta taggactggt cctgtattta ctggttgggg ggatgctgtt
     5401 ttcctacatc aggatgccaa gtcagtcctt attctagggt gagacttggg agtgggccta
     5461 tgaataagtt tcactataat ggggatagga gaaaaaacat gataaatatt gttgcattta
     5521 tggggccaag tggatccgga aagacaacgc tggccaagta tataaatatc aggaaggtta
     5581 ccacatacac cacaaggcct tcaagagagg gcgaaaggga cggcatagac tattacttca
     5641 tgaaggagga ggagctactg cgactct
//
LOCUS       DASRQF010000033         3009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_849584, whole genome shotgun sequence.
ACCESSION   DASRQF010000033 DASRQF010000000
VERSION     DASRQF010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849584"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="VFD79_00960"
     CDS             complement(<1..1133)
                     /locus_tag="VFD79_00960"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005398037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HZH92644.1"
                     /translation="MIESKVVAMLLAGGQGSRLKTLTKKIAKPAVPFGGKYRIIDFAL
                     SNATNSGIDDIGILTQYKPYLLNTHIGIGSSWDYDRNIGGLRILPPFTSEDGGRWYTG
                     TANAVFENIDFLDEMNPEYVLILSGDHIYKMDYTELLRAHKDKGADVSIAVMEVPWDE
                     ASRFGIINVDQENKIVEFEEKPENPKNNMASMGIYMFDWKQLRDYLIKDDENPDSDND
                     FGKNILPMMLEEGKKMYAWVFDGYWKDVGTVKSYWEANMDLLDPMNTLDLYDKDWRIY
                     TRTRNLPPQYLARNSIVKNALVNEGCYIRGTLMNSILFSDVVIEEEAVVVDSVVHSNC
                     VIEKGAKVYRAVISEGMTVKAGEVIGQENSENIYLVSEEGISV"
     gene            complement(1135..>3009)
                     /gene="glgB"
                     /locus_tag="VFD79_00965"
     CDS             complement(1135..>3009)
                     /gene="glgB"
                     /locus_tag="VFD79_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005398036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HZH92645.1"
                     /translation="ELDAYLFHEGTFYRAYEFMGAHPYKKGRKKGHRFVVWAPRAREV
                     YLTGDFNGWHEWSLPMERIPGSGLWSIVVQDVQDYDKYKYRIIAETGEVRYKADPFAF
                     HAGTRPETDSKVYDIKGFNWQDRKWMRTRKDNHQNSPVSIYEVNLLSWKMKEGGEPYS
                     YRELADDLVRYVKKMGYTHIELMPIAEHPLDASWGYQVTGYFAPTSRFGTPKDFMYLV
                     DQCHQNEIGVLLDWVPAHFTRDDHGLARFDGSYCFESLDRNRAENDQWGTLNFDYSKP
                     EVLSFLISNALYWHDYYHIDGLRIDAVAYMIYLDFGGRDIRNQYGGRENIEAIEFIKR
                     LNSVVFENYPGTMMIAEESTAWPKVSHPLDEGGLGFNYKWNMGWMNDTLKYMELDPIY
                     RKDHHQALTFSMMYAFSERFILPLSHDEVVHGKRSLLDKMPGFYEDKFANLRLLYQYM
                     YAHPGKKLLFMGGEFGQFIEWREYEGLDWHLLDYEMHQKLHKYVKDLNTLYKDEKCLF
                     EVDTSYEGFQWIEHQNWQESIISFERIDKDGNRLVCIFNFTPVPRPDYPIGVLEEGTY
                     RTVFTSDHQRYGGNTKRIKSYKATDEKFHDRPYSIRVELPPLGAMYLKLKEEKRTGGK
                     "
BASE COUNT          644 a          809 c          669 g          887 t
ORIGIN      
        1 tccaccgaga ttccttcctc agagacaagg tatatgttct ctgagttttc ctgtccgatc
       61 acttctcctg ccttgacggt cattccctcg gagatcaccg ccctgtagac cttggccccc
      121 ttctcgatga cacagttgga atggaccacg ctgtcaacta caacagcctc ctcctctatg
      181 accacatcac tgaagagtat gctgttcatc aaggtacccc tgatatagca gccctcgttt
      241 accagggcgt tctttacgat tgagttccgc gcaagatatt gtggtggcag gtttctggtt
      301 cttgtgtata tcctccagtc cttgtcgtaa aggtcaaggg tgttcatggg gtcaagcagg
      361 tccatgttgg cttcccaata gctcttgacg gttccaacat ccttccagta tccatcaaag
      421 acccaggcat acatcttctt gccttcctcc agcatcatcg gaagtatgtt cttgccaaag
      481 tcattgtctg aatcagggtt ttcatcatcc ttgatcaggt agtcccttag ctgcttccaa
      541 tcaaacatgt atatccccat tgaagccatg ttgttcttgg ggttttcagg cttttcctca
      601 aactcaacga tcttgttctc ctggtccaca ttgatgatcc caaacctgga tgcctcatcc
      661 caggggacct ccatgacggc gattgaaaca tcagccccct tgtccttgtg ggctctgaga
      721 agctctgtgt agtccatctt gtagatgtgg tctcccgaca gtatgagcac atactccgga
      781 ttcatctcat ccagaaagtc aatgttctca aagactgcat ttgctgtacc tgtgtaccat
      841 cgcccaccat cctccgatgt gaatggcggg agtatcctta agcctccaat gttcctgtca
      901 tagtcccatg atgagcctat gccaatgtgt gtgttgagaa ggtagggctt gtactgggta
      961 agtattccaa tgtcatcaat acctgaattt gttgcattac tcaatgcaaa atctatgatc
     1021 ctgtacttgc caccaaaggg cactgcgggt tttgctattt tcttggtcaa agtctttagt
     1081 cttgagccct gacctccggc cagaagcatt gcaactacct tgctttcaat catattactt
     1141 ccctcctgtc cttttttctt ccttcagctt cagatacatt gccccaagag ggggtagctc
     1201 aaccctgatg ctgtatggtc tgtcgtgaaa cttttcgtcg gttgccttgt aggattttat
     1261 tctcttggtg tttccaccat atctttgatg gtcacttgta aagaccgtcc tgtaggttcc
     1321 ctcctccagg actccgattg ggtagtctgg ccttggaaca ggggtaaagt tgaatatgca
     1381 tacaagcctg tttccatcct tgtctatcct ttcaaaggag atgatgctct cctgccagtt
     1441 ctggtgctcg atccactgga atccctcgta ggatgtatcc acttcaaaaa ggcatttctc
     1501 gtccttgtag agggtattca ggtccttgac gtacttgtgg agcttctggt gcatttcata
     1561 gtccagaagg tgccagtcaa gtccctcgta ctccctccat tcgataaact gtccaaactc
     1621 tccccccatg aagaggagct tcttgcccgg gtgggcgtac atgtattggt aaaggagtct
     1681 gaggttggca aacttatcct catagaagcc tggcatctta tccaagagtg atcgctttcc
     1741 gtgtaccacc tcgtcgtggc taaggggaag tatgaacctt tccgaaaagg catacatcat
     1801 actgaatgta agggcctggt ggtggtcttt cctgtagatg ggatccaact ccatgtactt
     1861 cagggtatcg ttcatccagc ccatgttcca cttgtagtta aagcccaggc ctccctcatc
     1921 aagggggtgg ctcacctttg gccatgcggt tgactcctcg gctatcatca ttgtgccggg
     1981 atagttctca aataccaccg aattaagcct ttttatgaac tcaatggcct cgatgttctc
     2041 cctgccgccg tactggtttc ttatgtccct gcctccaaaa tcaaggtaga tcatgtaggc
     2101 aacagcatct atcctaagtc cgtctatgtg gtagtagtca tgccagtaga gggcattaga
     2161 aatcaaaaag ctcaagactt caggcttgga gtagtcgaag ttcagggtcc cccactggtc
     2221 gttttcagcc ctgttcctgt ccagggattc aaagcagtag cttccgtcaa atcttgccag
     2281 accgtggtca tccctggtga aatgggcggg cacccagtca agaagcaccc ctatctcatt
     2341 ctggtggcat tggtccacca ggtacatgaa gtccttgggt gttccaaacc tgctggttgg
     2401 ggcaaagtaa cccgtgacct ggtatcccca ggatgcatcc aggggatgct ccgcaattgg
     2461 catcagttca atgtgtgtgt agcccatttt ctttacgtac ctgacaaggt catccgccag
     2521 ttccctgtat gagtagggtt ctcccccctc cttcattttc caggagagta ggttaacctc
     2581 atatattgac acgggagaat tctggtggtt atcctttctg gttctcatcc acttcctgtc
     2641 ctgccagttg aagcccttta tgtcgtaaac cttggaatcg gtctcaggcc ttgttcccgc
     2701 atggaaggca aaggggtccg ccttgtacct gacctctcca gtttcggcaa ttatcctgta
     2761 cttgtatttg tcgtaatcct ggacatcctg gacaactatt gaccaaaggc cgctgcctgg
     2821 tatcctctcc attggaagac tccactcatg ccaaccgttg aagtctcctg tcaggtagac
     2881 ctccctggcc cttggagccc agaccacaaa ccggtgacct ttttttctgc ccttcttgta
     2941 tggatgcgct cccatgaatt cataggccct gtaaaaggtt ccctcgtgaa aaaggtatgc
     3001 atccagttc
//
LOCUS       DASRQF010000034         2553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_848844, whole genome shotgun sequence.
ACCESSION   DASRQF010000034 DASRQF010000000
VERSION     DASRQF010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848844"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(286..1083)
                     /locus_tag="VFD79_00970"
     CDS             complement(286..1083)
                     /locus_tag="VFD79_00970"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZH92646.1"
                     /translation="MKSIIMKELRLQRKLIIIWSLILILTAAFGGIEFMGLQNQMDML
                     ETTVADFPRIVKIMFGVDAFPISTPLGGYASMFYWYQLVLFALAVYLGFHIVSRDEKD
                     NYADFIYTKPYDRKTVLLSKGTVVLIINAVFAVLTAVGTIIFLVPFLADGQTLMPHIM
                     TSTIGMFLTQLIFSAVGMVFGSITGHFRRGMLGGFLVLVVTYAIAFSIEYIGNLDHLN
                     FLSPVRYFNLVSLSQGGLSPLYLFLSAGLIGGLMFLAVKAIGRRDLI"
     gene            complement(1099..1905)
                     /locus_tag="VFD79_00975"
     CDS             complement(1099..1905)
                     /locus_tag="VFD79_00975"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZH92647.1"
                     /translation="MVVFKYELKQMKTLIIAWSVVMAALAFLLIPVFTDIGKSVTGDP
                     QQLEQMVSDNQFLQAVGMSAEFLSKPIGMYGFLNGWFFTLAASVLAMHVGLSVNTKDF
                     VGKSADFIYTKPLDRGKVFLSKLLAASVAVFTVGIVYFAASYLALRINLPEGFEMWMF
                     LHLAFSLIMLQAIYLALGIFVGVIWPRNRRPLLLAVTLVFITVVIGTFAATMSYDLLL
                     FLSPPKFFGGSVIASMGGYDMRFVYWLFFLVIAFISSGYFIFRKKDLITV"
     gene            complement(1918..>2553)
                     /locus_tag="VFD79_00980"
     CDS             complement(1918..>2553)
                     /locus_tag="VFD79_00980"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZH92648.1"
                     /translation="LSGEPSYYENMKAKEFLKYSAELYGRKDSSRLEELSERLQLDLK
                     RKIEDLSMGNKKKVGIISALLHSPKLLILDEPTSGLDPLMQQIFFDILREENENGTTI
                     LFSSHVLSEVQKLCNKVSIIKEGVIIDTQSIAELRKNGYKKVSILADGGLNEDDLKMD
                     GVADLNIRDGSASFIYMGPVNDLLQRINSFQVRDVFIEEPSLEEIFLHYYQ"
BASE COUNT          738 a          608 c          562 g          645 t
ORIGIN      
        1 atcaacctca gggtaaggac agacaatttc tccgccatat ctgcctacga aaagctgggc
       61 ttccatcagg agggaatcag gacaagggac ctttgcatag acggggtctt ccacgactca
      121 atctgcatgg gactggaaat agactaaaat tgaggaggca gcctgaaatt ctcaggctgc
      181 ctttaataat ggccattatt aatctgttac aaaatacctg ctatagtctc cagctaattt
      241 gctggcttca atcatacttt agctggcaag tatgacatgc tctgcctaaa tcaaatcccg
      301 cctgcctata gccttgaccg ccaggaacat caatcctcca ataagtccag ctgacaggaa
      361 aagatagagg ggactaagtc caccttggga cagagagacc aggttgaagt accttacagg
      421 ggacaggaag ttcaaatggt ccagattgcc aatatactct attgaaaatg cgatggcata
      481 ggtgactacg agaaccagaa agcccccaag catgcccctt ctgaagtgcc ctgtaatgga
      541 gccaaagacc atccccacag ccgaaaagat aagctgtgta aggaacattc caattgttga
      601 ggtcattata tggggcatca gggtctggcc atctgcaagg aatggaacca ggaatatgat
      661 cgttcccaca gctgtcaaca cagcaaatac agcatttatt atgagcacaa ccgttccctt
      721 tgagaggagt actgtttttc tgtcataggg cttggtatat atgaagtcgg catagttatc
      781 cttctcatcc cttgaaacaa tgtggaagcc aagatagacc gccagggcga acagcaccag
      841 ctggtaccag tagaacatgc tggcgtagcc accaagaggc gtacttattg gaaatgcatc
      901 cactccaaac atgatcttta ctattcttgg gaaatctgca actgttgttt caagcatatc
      961 catctgattc tgaagaccca tgaactcaat tccgccaaag gcggcagtaa gaatcaggat
     1021 aagggaccat atgatgatta gctttctttg aagccttaac tccttcatta ttatcgattt
     1081 caaatcacac acctcccttt agacggtaat aaggtctttt ttcctgaaaa tgaaatagcc
     1141 tgatgaaata aatgctatta ccaggaaaaa gagccagtat acaaatctca tatcgtagcc
     1201 acccatgctt gcaattaccg atccaccgaa gaatttggga ggggacagga acaagagcag
     1261 gtcatagctc atggttgcag caaaggtacc gattacaact gtaatgaaca caagggtcac
     1321 tgcaagtagt agtggccttc tatttcttgg ccatatgacc ccgacaaata ttccaagggc
     1381 aaggtagatt gcctggagca taataaggga aaaggcaagg tgaagaaaca tccacatctc
     1441 aaacccttcc ggcaggttta tccttagggc tagataggat gccgcaaaat atacaatccc
     1501 aacggtgaac acagccactg aagctgcaag tagctttgaa aggaagacct tgccacgatc
     1561 aaggggcttg gtgtatatga aatcggcaga tttgcccaca aaatccttgg tgttcacaga
     1621 cagacccaca tgcattgcca gaacagatgc tgccagtgtg aagaaccagc cgttgaggaa
     1681 gccatacatc ccaattggct ttgacaggaa ttcagcactc atgccgacag cctgcaggaa
     1741 ctgattgtct gaaaccatct gctcaagctg ctgggggtct cccgtaacgc ttttgccaat
     1801 atccgtgaac acgggaatca gcagaaatgc caaagctgcc ataacaacag accaggctat
     1861 aatcaaggtt ttcatctgct ttaattcgta tttgaaaact accacaacac tcacctccta
     1921 ctgatagtag tggagaaaga tttcctccaa gcttggttcc tctataaaca catccctaac
     1981 ctggaatgaa ttgatccttt gaagaaggtc attgacaggc cccatataga taaagcttgc
     2041 tgagccatcc ctgatattta ggtccgcgac tccatccatc ttgaggtcat cctcattcag
     2101 accaccatct gcaaggattg aaaccttttt gtatccattt tttctcagct cagctatgct
     2161 ctgggtatct atgataacgc cctcctttat tattgaaacc ttgttgcaga gcttctgcac
     2221 ctcactgagg acgtgggatg agaaaaggat ggttgttcca ttttcattct cctcccttag
     2281 gatatcgaag aatatctgct gcatcagcgg atcaagacca ctggttggtt catcaagaat
     2341 taggagcttg ggggagtgga gcagggcgga gatgattccg acctttttct tgtttcccat
     2401 tgaaaggtcc tcaatctttc gtttcagatc tagctggagc ctttctgaga gctcctcaag
     2461 cctggagctg tccttcctgc cgtagagctc agcggagtat ttcaggaatt ccttggcctt
     2521 catgttttca tagtagctgg gctctcctga cag
//
LOCUS       DASRQF010000035         7151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_481180, whole genome shotgun sequence.
ACCESSION   DASRQF010000035 DASRQF010000000
VERSION     DASRQF010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 7151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7151
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481180"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="VFD79_00985"
     CDS             <1..1032
                     /locus_tag="VFD79_00985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZH92649.1"
                     /translation="VIADGVNNMADSASSVVTLVGFKISGMPADKEHPYGHGRVEYIS
                     ALIVAFMVIMVGIQFIRSSVGRILDPVTVKFEIVSFVILTISILFKVWLALFNRNLGE
                     KINSQGLKATATDAMGDVLITSVVVMSIFFGQFTEIPVDGVVGVLVSLFIIYAGYSLV
                     KDTVSPLIGEAPSEELIASVYLDLMEYEYITGSHDLIIHSYGAGRTMATVDVEFPATI
                     DVVTIHDVIDRAERELGEKYNMTLVIHMDPLGPESVERYQLRKQVKKIVKADERVHSI
                     HDFQVLEEGDQRIVEFHTVVTDDNLSGDKEYEGLKKELEDAVDREIEGFKCRIILDID
                     FNGERENDI"
     gene            1022..2308
                     /locus_tag="VFD79_00990"
     CDS             1022..2308
                     /locus_tag="VFD79_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HZH92650.1"
                     /translation="MTYKKKLDMIENYIRSGEKKRQDFRVGVEMEHFVIWKDSLKTVS
                     YYGRDGVEEILRDLLPKGWKGTYEGDHLLGLRKNDIAITLEPGSQLEVSIDSARDIKD
                     LEKGYLEFLSEITPLLEKRNQVLVTLGYHPVTRIDEIRLLPKKRYDLMFNYFKTKGSH
                     AHNMMKGTGAFQVAIDYSDEKDYHKKFRVLNALAPVFYGIFENALFFEGEPCKTHNLR
                     AYVWMNCDNDRAGLVEGSLDVAFGYEDYARYILEKPPIFTVRNGDVIPTGDRKVKDIL
                     DPESTKKDELDHLMTMFFPDVRTKRYIEIRMMDSIPYPLNFSAVGLVKGLFYNQDKLD
                     KLYDFVGDITMDQVLDTKVEMLEKGMEAKLKGMTLLQIGKWLLDLAYEGLDQEERTYL
                     RPLEDLLIQGKNPYIHTRDRFEKGDRKYAIEWTMHE"
     gene            2321..3643
                     /locus_tag="VFD79_00995"
     CDS             2321..3643
                     /locus_tag="VFD79_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HZH92651.1"
                     /translation="MTGEKYQKMYLEMVINNQERYLDDYQKTVERVANSTAIYKNKPV
                     PFLYNPMFFSRMEEEDLKWIGSMIMEIGESVTDRFIQDQDFRKKFGFPDFIERMILRE
                     NRYGVHVPVGRFDVFYKDRDNFKFCELNTDGSSAMNEDNTIGDILLRSRALEDFGRKY
                     DLRNPELIKSWVKESISIYGKWDETGKKPNVAIVDFKESGTSAEFQLFKEAYENQGFS
                     CIIADPRDLEYRDGGLYHKDYRIDLIYRRIVTFELIEKYDQVEDFVEAYMDDAVCVVG
                     TIRSQVIHNKIFFKILHDQDTLDGLREDQREFVKRHIPFTGIFKGEEEVFQKVLKNKD
                     RYIVKPLDMNASQGVYPGRDFDREKWESILRDTFDRDYIYQEFVDPHKRDFVYYEDGR
                     FKAESLGSIVGIFMYNRHYSGLYTRLGKNNIISGVTEYYCAPNIIVNT"
     gene            complement(3689..4027)
                     /locus_tag="VFD79_01000"
     CDS             complement(3689..4027)
                     /locus_tag="VFD79_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HZH92652.1"
                     /translation="MKIAIASDKKKVAQHFGHCEAFECFTTDGNGVTGWDTIMNPGHK
                     PGFLPVYLHEKGIDVIIAGGMGQSASDLFTRNGIKVICGASGPIEKVAESYVDNTLQS
                     TGSLCEDHNH"
     gene            complement(4024..4899)
                     /locus_tag="VFD79_01005"
     CDS             complement(4024..4899)
                     /locus_tag="VFD79_01005"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZH92653.1"
                     /translation="MKQVLILSGKGGTGKTTLSSSLIKLSGAKAYADCDVDAPNLHLV
                     MDEKLEPNKSDFYGLPAAQIHTDKCISCDLCRQKCRFDAIDVTDVYTVDRFACEGCAV
                     CQLVCPADAIEMVDRVTGHLTLHENDHIFSTADLCMGSGNSGLLVTQVKKPLKNWHEK
                     APLAIIDGSPGIGCPVIASISGVDLVVLVAEPTLSGFSDMERIVETSRGFPAGVSVCI
                     NKYDLHLENTKIIENFCKDKGIPLLGKIPYDRETVSLVNQGKTLVDKKGPARDAIEEI
                     LVGVLGLINLKEDEI"
     gene            complement(4896..5732)
                     /locus_tag="VFD79_01010"
     CDS             complement(4896..5732)
                     /locus_tag="VFD79_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZH92654.1"
                     /translation="MKIAVLSGKGGTGKTFVSTNLSRVIEGSIYLDCDVEEPNGHLFL
                     KGPVLETNPVALPIPVIDHKLCDGCKKCVDFCQFNALAYTGKRVLVFEKICHSCGGCE
                     IICPQNAIKEIPREIGKIEKRSYEDTTLYTGILNTGEESGTAIIGELLQRVSEEESVV
                     IDCPPGSSCVVMDSIKDADYCLLVAEPSIFGAHNLKMVHELVTLFEKPMGIVLNKTLG
                     DENPSEDYCREKGLDILLRIPWSDELGRYNAEGIIASDQKDEYRNMFTDLLSRIHEEV
                     SV"
     gene            complement(5735..6094)
                     /locus_tag="VFD79_01015"
     CDS             complement(5735..6094)
                     /locus_tag="VFD79_01015"
                     /inference="COORDINATES: protein motif:HMM:NF014620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HZH92655.1"
                     /translation="MIIGIPADIEDMNGPVSGSFGRAPYYFIYDEEMRSGHFVQNTAA
                     TAQGGAGVKAAQILVDQKAEVVISPQLGENAAMVLKDSNMDLFKSVDGSLMDNVLLFR
                     DGKLEQLNDIHEGFHKH"
     gene            complement(6106..6417)
                     /locus_tag="VFD79_01020"
     CDS             complement(6106..6417)
                     /locus_tag="VFD79_01020"
                     /inference="COORDINATES: protein motif:HMM:NF036294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HZH92656.1"
                     /translation="MPRFDMTGPMGTGPFTGKGMGYCAQRAYGGMGYGRGAGYGRALG
                     YGRGVGYGRGMGFRRGPAFGPAYYDMPVGTEKEMLEEDLAYLEDQLAAVKEALAKMDT
                     E"
     gene            6684..>7151
                     /locus_tag="VFD79_01025"
     CDS             6684..>7151
                     /locus_tag="VFD79_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HZH92657.1"
                     /translation="MKAMIVGAGKLGSKLAEFLIAENIDVTVMDSNTRVLERLNEHLD
                     VLTVVANGIDIRMMRELEIQEYDLLVGSTENDETNTLICTLAKKLGCKQTIARIRNPE
                     YMQQIDFIKAELGIDHIVNPDLATAIAMEKYLMKSYSFYTDEFASGKVQMVDFN"
BASE COUNT         2056 a         1505 c         1681 g         1909 t
ORIGIN      
        1 gttattgcag atggggtcaa caacatggca gactcagcat catctgttgt gacacttgtt
       61 ggattcaaga tatccggaat gcctgcagac aaggaacatc catacggtca tggcagagtt
      121 gaatacattt ccgccctaat agtagcattt atggtaataa tggtggggat acagttcata
      181 aggtcctccg tgggaaggat acttgatccg gtgacggtta agtttgagat tgtatccttt
      241 gtaatcctga ccatatcaat actattcaag gtgtggctgg ctttgttcaa tagaaacctg
      301 ggagaaaaaa tcaactctca gggtctcaag gccactgcta cggatgccat gggggatgtt
      361 ctaataacat cggttgttgt catgtcaata ttttttggcc agtttacgga aattcccgtg
      421 gatggagtgg taggtgtact tgtttcactt ttcataatct atgcaggcta tagtctggtc
      481 aaggatactg ttagcccttt gattggagag gcaccttctg aggaactgat tgcgtcggtc
      541 tatttggacc ttatggaata tgaatatatt acaggatcac atgacctgat aatacactcc
      601 tatggagcag gaagaacaat ggctacagtt gatgttgagt tccccgctac cattgatgtt
      661 gtaaccatac atgatgtaat cgacagggct gaaagggagc tgggagaaaa gtataacatg
      721 accctggtga ttcacatgga tccactggga cctgagagcg ttgaacggta ccagttgcgg
      781 aagcaggtca agaagatagt gaaggctgat gaaagggtcc actcaatcca tgattttcaa
      841 gtcctggagg agggagatca aagaattgtt gagttccaca ctgtggtaac agatgacaac
      901 ctttctggag acaaggaata tgaaggcctt aagaaggaac ttgaagacgc tgtggatagg
      961 gagattgaag gctttaagtg taggattatt ctggatatag attttaacgg ggagagagag
     1021 aatgacatat aagaagaagc tggatatgat agagaactac atcaggagcg gtgaaaaaaa
     1081 gaggcaggac ttcagggtag gagttgaaat ggagcatttt gtaatctgga aggattctct
     1141 aaagacagta tcctactatg gcagggacgg ggtggaggaa atactcagag acctgctgcc
     1201 aaaggggtgg aaggggacct atgagggtga tcatctcttg gggcttagga agaatgacat
     1261 tgcaataacc cttgagcctg gtagccagct tgaggtgagc atagactccg ccagggacat
     1321 caaggacctg gaaaagggtt atctggaatt tttgtcagaa atcacaccac tccttgaaaa
     1381 aagaaatcag gttcttgtta ccctgggcta ccatccagtt accaggattg atgaaataag
     1441 gcttctacca aaaaagaggt atgacctgat gttcaactat ttcaagacta aggggtccca
     1501 tgcacacaac atgatgaagg gaacaggtgc atttcaggtg gccatagatt acagtgatga
     1561 aaaggactac cacaaaaaat tcagggtgtt gaatgccttg gctcctgttt tttacgggat
     1621 tttcgagaat gccctcttct tcgaggggga gccctgcaag acccacaatt taagggccta
     1681 tgtctggatg aactgcgaca atgacagggc aggacttgtt gaggggtccc tggatgtggc
     1741 cttcggatac gaggattatg ccaggtatat actggaaaaa cctccgattt tcactgttag
     1801 aaatggagac gttatcccta cgggggatag gaaggtcaag gatattttgg accctgagtc
     1861 gacgaaaaag gatgagttgg accatctgat gaccatgttc tttccagatg ttagaacaaa
     1921 gagatacatt gaaataagaa tgatggattc cattccatac ccattgaatt tttcggctgt
     1981 tgggctggtg aagggacttt tctacaacca ggacaaactg gacaagcttt atgactttgt
     2041 gggggatatc accatggacc aggtactgga tacaaaggtg gagatgctgg agaagggcat
     2101 ggaggcaaag ctgaagggaa tgaccctgct tcagattgga aagtggctac tggacctggc
     2161 atatgaaggt ttggaccagg aggaaaggac atatcttaga cctctggaag accttcttat
     2221 acagggcaag aacccatata tccacacaag ggacaggttt gaaaagggtg acaggaagta
     2281 tgcaatagaa tggacaatgc acgaataatg gggtgaagct atgacaggtg aaaaatatca
     2341 aaaaatgtac ctggagatgg ttatcaacaa ccaggagagg tacttggatg attatcagaa
     2401 gacagtggag agggtggcca attcaactgc aatatacaag aacaagcctg taccatttct
     2461 atacaatcca atgttttttt ctcggatgga ggaagaagac cttaaatgga ttgggagtat
     2521 gatcatggag ataggtgaga gtgtaacaga taggtttatt caggaccagg atttcaggaa
     2581 aaagtttggc tttccagact ttatcgaaag gatgatacta agggaaaaca ggtatggtgt
     2641 ccacgttccc gtaggaagat tcgatgtttt ctacaaggac agagacaact tcaagttctg
     2701 tgagctaaac accgacggat catccgccat gaacgaggac aatacaatcg gggatatcct
     2761 cctcaggtcc agggcattgg aggactttgg acggaagtat gacctgagaa atccggagct
     2821 tatcaaaagc tgggttaaag aatccataag catatatggc aaatgggatg aaactggtaa
     2881 gaaaccaaat gttgcgattg ttgatttcaa agaaagcgga acttcagcag agtttcagct
     2941 gttcaaggaa gcctatgaaa accagggctt cagctgcatc atagcagatc caagagattt
     3001 ggaatacagg gatgggggac tgtatcacaa ggactacagg atagacctta tctacagaag
     3061 aatagtcacc tttgaactaa ttgagaagta tgaccaagtg gaggactttg tggaggccta
     3121 tatggatgac gcagtttgcg tagttggaac cataaggtcc caggtgatcc acaacaagat
     3181 tttctttaag atcctccatg accaggatac cctggatgga ctgagggaag atcaaaggga
     3241 atttgtcaaa aggcacattc cctttacagg tattttcaaa ggggaagagg aagtgtttca
     3301 gaaggtcctt aagaacaagg ataggtacat tgttaagccc ttggatatga atgcatccca
     3361 gggtgtctac ccaggcaggg attttgaccg ggaaaagtgg gagtcaatac tgagggatac
     3421 ctttgacagg gactacatct accaggaatt cgtagaccca cacaagagag actttgtcta
     3481 ttatgaggac ggcaggttca aggcagagag cctgggctcc attgttggga tattcatgta
     3541 caataggcac tacagtggac tttacactag acttggaaag aacaatatca tatctggtgt
     3601 aaccgaatac tactgtgctc caaatattat cgtaaacacg tagaaatgac cgggaaatcc
     3661 cggtcatttc tacgtgttag aatatacatc aatgattgtg gtcctcgcaa agggagcctg
     3721 tggactggag agtgttatcc acatagctct cagcgacctt ttcaattggt cccgaggcac
     3781 cacagattac cttgatcccg tttcttgtga acaggtctga agcactttga cccattcctc
     3841 ctgcgattat tacatcaatg cccttttcgt ggagatatac tggcagaaat cctggcttgt
     3901 gacctggatt cataatggta tcccatccgg tcacaccatt tccatcagtg gtaaagcact
     3961 caaaggcttc acagtgtcca aaatgctgag caaccttctt tttatccgat gctatggcta
     4021 tcttcatatt tcgtcctcct ttaaattgat taatcccaat acccccacca atatctcttc
     4081 tattgcatcc cgtgcaggtc ctttcttgtc cacaagggtt tttccctgat ttactagact
     4141 gaccgtttct ctgtcatatg gtatctttcc cagaagtggg atgcctttgt ccttacagaa
     4201 attttcaatt atttttgtat tctccaagtg gaggtcatat ttgtttatgc aaactgaaac
     4261 tcctgcagga aagcccctag atgtttcaac tattcgttcc atatcactga agcctgaaag
     4321 tgtaggctct gccacaagaa ccacaaggtc cacaccgctg atggatgcaa ttacagggca
     4381 tccaattcct ggagaaccgt caattatggc taggggtgcc ttttcatgcc aattctttaa
     4441 gggtttttta acctgggtta caagtagccc cgagtttccg cttcccatgc ataggtctgc
     4501 agtggagaaa atatgatcat tctcatgaag tgtgagatgt ccagtaaccc tatctaccat
     4561 ctctatggca tcagcagggc atacaagctg gcatacagca caaccttcgc aggcaaacct
     4621 gtcaacagta tatacatccg tcacatctat tgcatcaaac cgacacttct gtctgcacag
     4681 atcgcaggaa atgcatttgt ccgtatggat ctgcgccgct gggagcccgt agaaatcaga
     4741 tttgtttggc tccagctttt catccataac aagatgaagg tttggagcat ccacatcgca
     4801 gtcagcatat gcttttgctc ctgagagttt tatcaatgaa ctggaaagcg tggtcttacc
     4861 tgttccaccc ttaccgctaa gtataagtac ctgcttcata cagacacctc ctcatgaatt
     4921 ctactgagaa gatctgtaaa catatttctg tattcatcct tctggtcaga cgcaataatc
     4981 ccctctgcat tgtaccttcc aagctcgtcg ctccaaggaa tccttaggag aatatccagg
     5041 cccttttccc tgcagtagtc ctctgacgga ttctcatcac ccagggtctt gttaagtacg
     5101 attcccatgg gcttttcaaa aagggtgaca agctcgtgga ccatcttcag gttatgggct
     5161 ccgaatattg aaggctcagc aacaagaaga caataatccg catcctttat ggaatccatc
     5221 acaacacagg aacttccagg gggacagtct atgaccacgg actcctcctc agaaactctt
     5281 tgcagcagtt ctcctatgat tgctgttcct gattcctcac ctgtgtttag tatcccggtg
     5341 taaagtgtgg tgtcttcata ggatcgcttc tcaatcttgc ctatttctct tggaatctcc
     5401 ttgattgcat tctgtggaca gattatctcg cagccaccgc aggagtgaca tatcttttca
     5461 aaaaccagaa ccctcttacc cgtgtatgct agagcgttga actggcagaa atccacacat
     5521 ttcttacagc cgtcacagag cttgtggtct attacaggta tggggagggc aaccggattg
     5581 gtctcaagga ccggaccctt tagaaacaga tggccgtttg gttcctccac atcacagtcc
     5641 agatatattg agccttctat aactctggag agatttgtgg atacaaaggt cttcccagtg
     5701 cctcccttac cacttaaaac tgcgatcttc atatttagtg cttgtgaaac ccttcgtgga
     5761 tatcattcag ctgctcaagc ttgccatccc taaacaggag cacattatcc ataagacttc
     5821 catccacaga cttgaaaaga tccatgtttg aatctttcaa gaccatggct gcattctctc
     5881 caagctgagg agatattaca acttcggcct tttggtcaac aagaatctgt gcagccttta
     5941 cacctgctcc accctgggcg gttgcagcgg tattctgaac aaaatgtcct gatctcatct
     6001 cctcgtcata gataaaataa tatggtgccc taccgaatga accactgaca ggtccattca
     6061 tatcctcaat atctgctggt atgcctatta tcattccatg acctcctatt cagtatccat
     6121 ctttgccaga gcttccttaa ctgcagcaag ttggtcttca agatatgcca gatcctcttc
     6181 cagcatttcc ttttcagttc ctacgggcat atcgtaatag gccggaccaa atgctggccc
     6241 tcttcgaaaa cccatacccc gtccatagcc aacgcctctt ccataaccca gggcacgtcc
     6301 ataacctgct cctcgtccgt agcccattcc tccatacgct ctctgagcac aatatcccat
     6361 acccttaccg gtgaatgggc cagtgcccat tggtcctgtc atatcaaatc ttggcataat
     6421 aatactcctt cctaattgat aatgattatc tccttctacc tttattatac tctaatatgg
     6481 gcatatgtca ataaacattc ccaaaaagat atggtgtgaa taagttggaa tcttgattgg
     6541 aatatagtat actgtaattg acaaagtatg cataaaacca tagaggatta aaagaaggcc
     6601 ggtgagtgat tcaccgcctg ttgttttact gatagatatt tagtatagtt aaattttcat
     6661 taacaggtag aaggggtgga accatgaagg caatgatagt tggggcagga aagctgggat
     6721 ctaagctggc tgagtttctg attgctgaga atatagatgt aactgttatg gatagcaaca
     6781 ccagggttct ggaaaggctt aatgagcatc tggacgtgct taccgttgta gccaatggca
     6841 ttgacataag aatgatgaga gagttggaaa tacaggagta cgaccttctt gtggggagta
     6901 ccgaaaatga cgagaccaat accttgattt gtaccctggc aaaaaaactg ggctgcaaac
     6961 agacaatcgc aaggataaga aatcctgagt acatgcagca gattgatttc attaaggctg
     7021 aactgggaat agaccatatt gtgaatccgg accttgcaac cgcaattgcc atggaaaagt
     7081 acctgatgaa gagctacagc ttctacacag atgaatttgc cagcggcaag gttcagatgg
     7141 tggattttaa t
//
LOCUS       DASRQF010000036         2969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_100117, whole genome shotgun sequence.
ACCESSION   DASRQF010000036 DASRQF010000000
VERSION     DASRQF010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100117"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VFD79_01030"
     CDS             <1..711
                     /locus_tag="VFD79_01030"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HisS family protein"
                     /protein_id="HZH92658.1"
                     /translation="VEEELEANGLSQDQIHSIKRFIQIEGSNDEIIDALSEMRIDNDL
                     FKEGLDEIETVVKYLRLFEVPESNFAIDLTIARGLDYYTGTVYETLLDEYPSIGSVCS
                     GGRYDNLATYYTKQQLPGVGISIGLTRLFYQLMEAGIINTQESSLTKVLVIPMEGYMD
                     ESIKLAAALRKEDIFTQIYLEETKLGKKFNYADKMGIPFVIVIGEEEKKTGEYTLRDM
                     KSGQQTKYDLNSLIEVLK"
     gene            1014..2921
                     /gene="thrS"
                     /locus_tag="VFD79_01035"
     CDS             1014..2921
                     /gene="thrS"
                     /locus_tag="VFD79_01035"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019116862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HZH92659.1"
                     /translation="MIKITLPDGSVREYEDGVKVYDVTADISEGLARVAMGAVVNGKI
                     LGMQETIGEDSDFRVVKFEDPEGKKIFWHTSAHIMAHAVQNLFPDVKFAIGPAIENGF
                     YYDFDTEHRFTPEDLERIEEEMKSVAKEGHVLERFEMPRNEALDYFKEKGEEYKVDLI
                     ENLPEDEIISFYQLGEFTDLCAGPHLYDTRKVKAIKLMSIAGAYWRGDENNKMLQRIY
                     GTTYEKKKDLEEYLNRLEEAKKRDHRKLGKELDLFSMQEEGPGFPFFHPKGMVIRNIL
                     ENFWRDEHTKRGYGEIKTPLILNEQLWHQSGHWDHYKENMYFTSIDEGAYAIKPMNCP
                     GSILVYKSKMYSYRDLPLRWGELGLVHRHELSGALHGLMRVRSFTQDDAHLYMLPSQV
                     KDELKKTIDLADYIYDVFGFKYNIELSTRPENSMGTEEQWNLATESLREALEEKEIPF
                     KINEGDGAFYGPKIDFHLEDAIGRTWQCGTIQLDFQMPERFDLTYVDQDNEKKRPVMV
                     HRTILGSMERFMGILIEHFAGKFPVWIAPVQATVLPISDKFNDYAKEVAKQLEAKGIR
                     VEVDERAEKIGYKIREAQMQKLPYMLIVGEKEVEDRLVSVRKRDQGDMGQMGIEAFIE
                     QIMEEIESKAK"
BASE COUNT         1007 a          496 c          740 g          726 t
ORIGIN      
        1 gtggaggaag agcttgaagc taatggtttg tcacaggacc aaatccacag catcaagagg
       61 tttatccaaa ttgagggaag taatgatgaa ataatagatg ccttaagtga aatgagaatt
      121 gataacgatc tgtttaagga aggtctagat gaaatagaaa ctgttgtcaa gtatttgaga
      181 ttatttgagg tcccagaatc taattttgca attgatctta caattgcaag ggggctggat
      241 tactatacag gcacagttta tgaaaccttg cttgatgaat atccatcaat tgggagtgta
      301 tgctcaggcg gaagatatga caacctggct acctactaca ccaaacagca gctgccaggt
      361 gttggaatat caattggcct gaccagactg ttctaccagc taatggaagc tgggataatc
      421 aacactcagg aaagctctct cacaaaggtt ctagttatcc ccatggaggg atacatggac
      481 gagtcaataa agcttgcagc tgcattgaga aaagaggaca tcttcacaca aatatatctc
      541 gaagagacta aacttggcaa gaaattcaac tatgcagata aaatggggat cccttttgtc
      601 atagtaatag gtgaagaaga gaaaaaaacc ggagagtata cactgaggga catgaagtct
      661 ggtcaacaga ccaagtatga cttgaacagc ttgattgagg ttttaaagta gtacatttgt
      721 ggtatataca ataatagctt gacaagcttg gtcaaaattg ttaaaattta tctaattaaa
      781 aatcaaaggt gtagatggga agagtaagtt gatttcaatt gtcagagaga gaaaacccag
      841 gctgaaagtt ttcttcaagt ggattgacct aaaaccaccc cggagccgta cctgaaaagg
      901 taaccgccac aaggcgttat tttaaatgag tataaattag ggtggaacca cggtctttcg
      961 tccctacaga aatgtaggta tgaaagacct tattttttta aggagggaaa attatgatta
     1021 agattacttt acctgatggt tcagtaagag agtacgaaga tggtgtaaag gtgtatgatg
     1081 taacggctga tatttcagaa ggtcttgcaa gggttgccat gggagcagtg gtaaatggca
     1141 agatactggg aatgcaggag acaatcggcg aggattcaga ttttagggta gttaaattcg
     1201 aagatcctga aggcaagaag atattttggc atacttccgc ccatataatg gctcatgcgg
     1261 ttcaaaatct tttcccagat gttaagtttg ccattggacc agccattgag aatggattct
     1321 attacgactt tgatacagag cacagattta caccagagga ccttgagagg attgaagagg
     1381 aaatgaagag tgtagccaag gaaggccacg ttctcgagag atttgaaatg cctaggaatg
     1441 aagcattgga ttatttcaag gaaaaaggag aagagtacaa ggtggatttg attgagaatc
     1501 taccagagga tgaaatcatt tctttctacc agcttggaga attcactgac ctttgtgctg
     1561 gacctcacct ttacgataca aggaaggtca aggcaatcaa acttatgagc attgctggag
     1621 catactggag aggtgacgaa aacaacaaga tgcttcaaag gatctatggt acaacctatg
     1681 aaaagaagaa agaccttgaa gaatacctta atagacttga agaggcaaaa aagagagacc
     1741 acaggaaact aggcaaggag ctggacctgt tcagcatgca ggaagaagga cctggattcc
     1801 cattcttcca tccaaaggga atggtaatca gaaatattct tgaaaacttt tggagggatg
     1861 aacatacaaa aaggggatat ggtgaaataa agacacctct gatccttaat gagcaactat
     1921 ggcatcagtc aggtcattgg gaccactaca aggagaacat gtatttcaca tccatagatg
     1981 agggtgccta tgcaataaag cccatgaatt gccctgggtc gatacttgta tacaaatcaa
     2041 agatgtacag ctacagggac cttccattga gatggggaga gctggggcta gttcaccgcc
     2101 acgaactttc aggagcatta catggcctta tgagggtaag aagctttact caggatgatg
     2161 cccacctgta tatgctacct tcacaggtca aggatgaact taaaaagact atagatcttg
     2221 cagactacat ctacgatgta ttcggattca agtacaacat tgaactgtca accagacctg
     2281 aaaattcaat gggaactgaa gagcaatgga atcttgccac tgaaagctta agagaggcac
     2341 tggaggaaaa ggagattcca ttcaagataa acgaaggtga cggtgcattc tatggcccta
     2401 agatagactt tcaccttgag gatgcaattg gaaggacatg gcagtgtgga actatacagc
     2461 tggacttcca gatgccggag aggtttgacc tgacatatgt ggaccaggac aatgagaaga
     2521 agagacctgt aatggttcac agaacaattc ttggaagcat ggaaagattt atggggattc
     2581 ttattgagca ctttgcaggg aaattccccg tttggattgc acctgttcaa gctactgttc
     2641 taccaatatc agacaagttc aatgattatg ccaaagaggt ggcaaaacaa ctggaggcca
     2701 agggtatccg tgttgaggtt gatgaaagag ctgaaaagat tggatacaaa attcgagagg
     2761 cacagatgca aaagctgcct tatatgctga tagtaggaga aaaggaagtt gaggacaggc
     2821 tagtatcagt aagaaagagg gatcagggcg atatgggtca gatgggaatt gaagccttca
     2881 tcgaacagat aatggaagag attgaaagca aagccaaata gaccggaggt tagggatgga
     2941 ttttaaggac actattagga agctagagc
//
LOCUS       DASRQF010000037         2095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1840742, whole genome shotgun sequence.
ACCESSION   DASRQF010000037 DASRQF010000000
VERSION     DASRQF010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1840742"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            267..1319
                     /locus_tag="VFD79_01040"
     CDS             267..1319
                     /locus_tag="VFD79_01040"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HZH92660.1"
                     /translation="MFENIYTYLMDHLLLPAVFIVAVIVVRPIINRILKKWDINSHLE
                     NWVALYALLVFFLSYYQNSNWLFYSFITLGSIGINLNLLIVSFLIITMAFHISKIFTK
                     QFLPSIYTRYQLDRGVQFTFNRLLHYIIMIVAILMTASAVGINLNSLTVFAGVLGVGI
                     GFGLQNITSNFISGIILLFERPIKIGDRVIVEDIVGDVEQINMRATIIRSINNEHIIV
                     PHSYFLEEHVINRSHGSPYMRLIAPVGVSYDSDPEFVKELLLDVVYSEAKENPSVLLD
                     PSPSVHFDGFGASSLNFDLMVWVTDPKQLRRVKTNLNFRIFKSFKKNGVEIPFPQHDL
                     HLRSVDRDLLEKHLNK"
     gene            complement(1468..1887)
                     /locus_tag="VFD79_01045"
     CDS             complement(1468..1887)
                     /locus_tag="VFD79_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92661.1"
                     /translation="MSGKVETYVDLLSKRDSGVRLNFDEVSDEQVIHFCLEEDLIDAE
                     IAYLYNTTTDVVRKRRQQAYLNQIHRLQEKVLQEQFVVMAKNEKEKDLGSIKSDNVRD
                     LIGQIHSLSNSELDELLDYLIMNNKRLSSTIIRPGKV"
BASE COUNT          600 a          488 c          368 g          639 t
ORIGIN      
        1 tgaggttatg gccaagaatg ccatggcaga tgcatgccag gcaacaaatc ctagaacagc
       61 aacgcttgat gaggttattc agatattcaa gaatgcaatg tagactaaat atacaaaaaa
      121 cgtactgtgc ttgctcccag gagcatactc agtacctttt ttatgccatt ctaactccat
      181 caaaatctca tgacttcatt tccatttact tgcaatccaa ctccccttcc attaaaatat
      241 actcaaacac ttagaggagg ttgtcaatgt ttgaaaatat atatacctac ctaatggacc
      301 atcttttact gccggcggtt ttcatagttg cagtaatagt ggtcagacca ataataaaca
      361 ggattctaaa aaaatgggat atcaattcac atctggagaa ttgggttgcc ctctatgcct
      421 tactggtctt tttccttagc tattaccaga attccaattg gttgttctat tcctttataa
      481 cccttgggtc cattggcata aacctcaacc tactgattgt atcctttctc ataataacca
      541 tggcatttca catatcaaaa atctttacaa agcagtttct acctagtatt tacacccggt
      601 accaacttga cagaggcgtc cagtttacat tcaacaggct gcttcattat ataatcatga
      661 tagtggcaat cctaatgacc gcctctgcag ttggaataaa tctaaattca cttactgtat
      721 ttgctggagt tctaggtgta ggtatcggat ttggactaca aaacatcaca tccaacttca
      781 tatcaggaat aattctactg tttgaaaggc ccataaagat cggcgaccgt gtgattgtag
      841 aggatatagt cggtgatgtg gaacagatca atatgcgagc taccattatc aggtccataa
      901 acaacgaaca tatcattgtt ccacactctt actttcttga agagcatgta atcaacaggt
      961 cccatggctc accatatatg cgacttattg cacctgttgg ggtctcctac gattcagacc
     1021 cagagtttgt gaaggaactt ctcctggatg ttgtatactc ggaggcaaag gaaaaccctt
     1081 ctgtcctcct tgaccccagt ccatctgtac acttcgatgg ctttggtgcc tcctccctga
     1141 atttcgacct tatggtatgg gttactgatc ccaagcagtt acgcagggtc aaaacaaatt
     1201 tgaatttcag gatattcaag agcttcaaga aaaacggcgt ggagatacca tttccgcagc
     1261 atgatcttca cctccgttca gttgataggg acctcctgga aaagcacctg aataagtaag
     1321 atcaataaca atcaagctca ttacatttgc gatgagcttg atttactttt gtctgagttc
     1381 tagaagaagt ttagcaaaag gacaatcata atcaggagca tgacgctgta tataaaaagt
     1441 aaaaataacc gtcccccatg ctttttgtta aaccttccct ggtctgatta ttgtggatga
     1501 aagcctcttg ttgttcatta tcaggtaatc aaggagctca tccagctccg agtttgagag
     1561 gctatgaatc tgcccaatca ggtccctaac attgtccgac ttgatggatc ccaggtcctt
     1621 ctccttttca ttctttgcca ttaccacaaa ctgttcctgg agaaccttct cctgaagtct
     1681 atgtatctgg tttagatatg cctgttgtct tctcttgcga acaacatctg tagttgtgtt
     1741 gtacaagtat gcaatttcag catcaatcag gtcttcctca aggcagaaat gaataacctg
     1801 ttcatcggat acctcatcaa aattcaacct aaccccagag tccctcttgc tcagtagatc
     1861 tacataggtt tcaaccttgc cagacattcc atctccccct tttggaaatg ccacttgtta
     1921 taatccgttt ttaggtatat acccacaaca gcacaaaaac catacagcca ggccatatgg
     1981 ttctaaatgt acaatattct tattttttat caataatgtc ctttattatc ccgattccct
     2041 tttcaagctg ccacccttca acaaactcat cctccgaatg ggcctggtct atgct
//
LOCUS       DASRQF010000038         2152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1220147, whole genome shotgun sequence.
ACCESSION   DASRQF010000038 DASRQF010000000
VERSION     DASRQF010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220147"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..839)
                     /locus_tag="VFD79_01050"
     CDS             complement(60..839)
                     /locus_tag="VFD79_01050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZH92662.1"
                     /translation="MIERLEKDRWDSIIPELMEHTVENYFNLLGLLSKKPIYRDIYIQ
                     RNDEGRIVSYLFHRLSGTIKFYARGDYDTAELSKFMEKLDFKKLIGPFSSTEALEGAS
                     LLERGKPITFLCRLEIPLGPEVGKTRGIRQLGADDLEKVVEVYLEVFKSFASEEVIEE
                     KLRTGRGRAYGLWDGDRLVSVVQTDFEQETSALIVGVATRLNQQGRGFGTVLMSHVIR
                     ILHSEGKKIYLEYENPEAGGLYRKLGFTQFDTVMEYNRILL"
     gene            complement(850..1812)
                     /locus_tag="VFD79_01055"
     CDS             complement(850..1812)
                     /locus_tag="VFD79_01055"
                     /inference="COORDINATES: protein motif:HMM:NF016983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HZH92663.1"
                     /translation="MDNNQDLLKGIARVGIKIGIGLLVLLLIIIFNQTVQIVNFMNTI
                     NPILGKATLVVLLVLFASLLMMPLVGFLRYKRGMEIPEDEDSQEFRDYLKQTQKNMLR
                     NKYLKGENFRFDPEKDLKDEVVRAYGVLDERARTSIKDKATGVFLTTAISQNGVLDGF
                     FVLGALTKMVWELATLYETRPSLNRMLALYGNVAATVLMARGVEDLDLLDEQIEPLVA
                     SILGGGMATLVPGAVTVTNIIVNSITEGSVNALLTLRVGCIAQRYLSALTKPDKKSLR
                     RIATIEALSMLGSVLKDNTVTILKAFSKGATNAARNTVKNTFRF"
     gene            complement(1827..>2152)
                     /locus_tag="VFD79_01060"
     CDS             complement(1827..>2152)
                     /locus_tag="VFD79_01060"
                     /inference="COORDINATES: protein motif:HMM:NF024278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OST-HTH/LOTUS domain-containing protein"
                     /protein_id="HZH92664.1"
                     /translation="TEKGMTDINVIKLAIKKMLNEADGKDQRMNMGEIGNRLAKRYSD
                     FDVRNYGDTKLSKFLKKMDFLEIETDKEGGTAMWVALKGESEENKSSSNQQNRKRSYK
                     KRKKT"
BASE COUNT          513 a          563 c          401 g          675 t
ORIGIN      
        1 tattagttaa tttcttaaca atgtgaagtt aagagcatct gcaccatgga cagatgttct
       61 tataacaaga ttctattgta ttccataacg gtatcaaatt gtgtgaagcc tagctttcta
      121 tacaggccac cagcttccgg attctcatat tccaggtaga ttttcttgcc ctctgaatga
      181 agaattctta tcacatggct catcaacact gtcccaaagc ccctcccttg ttgatttaat
      241 cttgtggcaa caccaacaat caatgcagag gtctcctgct caaaatcagt ttgcacaaca
      301 gaaaccagcc tatccccatc ccagagtccg tacgcccttc ccctaccggt tctcagcttt
      361 tcctctatta cttcctctga tgcgaaggat ttgaatacct ccagatatac ttccaccact
      421 ttctccagat catcagctcc aagctgtcgt atacccctgg tctttcctac ttcaggccct
      481 aatggtattt caagcctaca caggaaggta attggttttc ccctctccaa tagactggcc
      541 ccttccaagg cttctgtaga tgagaaagga ccaatgagct ttttgaagtc aagtttctcc
      601 ataaacttag atagttcagc cgtatcatag tctcccctgg cgtagaactt gattgttcca
      661 gacagcctgt gaaaaaggta gctgacgatt ctcccttcat cattcctctg gatgtagatg
      721 tccctgtata ttggtttttt tgataaaaga cccagaaggt tgaagtagtt ttcaacggta
      781 tgctccataa gctcgggaat tatggagtcc cacctgtctt tttcaagcct ttcaatcata
      841 tgatcactcc tagaacctaa aggtgttctt aacggtattt ctggctgcgt ttgtggcacc
      901 cttgctgaaa gccttcagaa ttgtcacagt attgtccttg agtaccgaac cgagcattga
      961 caatgcttct atggtggcga ttctccttag gctttttttg tctggctttg taagagcact
     1021 taggtatctt tgggctatgc atcccaccct aagggtgagc agggcgttta ctgagccttc
     1081 cgtgatggaa ttaactatta tattggttac agtaacagct cccggtacca gggttgccat
     1141 ccctcctccc aggatggagg ccaccaaggg ttcaatctgc tcatccaaaa gatccaggtc
     1201 ctcaactccc cttgccatca ggacagtggc tgcaacattc ccgtacaggg ctaacatcct
     1261 gttcaatgat ggccttgtct catagagggt tgcaagctcc cataccatct tggtcaaggc
     1321 tcccagaacg aagaatccat ccagcactcc attttgggag atggctgtgg tcaaaaacac
     1381 acctgttgcc ttgtccttta ttgaggtcct tgccctttca tccagcactc catatgccct
     1441 gaccacctca tccttgaggt ccttttcagg gtcaaaccgg aagttctccc ccttgagata
     1501 cttgttcctt aacatgttct tttgggtctg tttcaggtaa tccctgaatt cctgggaatc
     1561 ctcatcctca ggaatctcca tacccctttt gtaccttaag aatccaacca agggcatcat
     1621 cagcagggat gcaaacaaga caagaaggac caccagagtg gccttcccca gtattggatt
     1681 tattgtattc atgaaattga caatctggac cgtctgattg aagattataa tcaagagcag
     1741 gacaagtaat ccaattccga tctttatacc cactcttgca atacctttta ataaatcctg
     1801 gttattatcc atggtctatc tcccgtttag gttttctttc tcttcttgta gcttctcttc
     1861 cgattctgct gatttgagct ggatttgttt tcttcgcttt cacccttcag ggctacccac
     1921 attgccgtac cgccttcctt gtcggtttca atctcaagaa agtccatttt cttgaggaac
     1981 ttggaaagtt ttgtatcccc gtagttccta acatcaaaat cagagtatct ctttgccagc
     2041 ctgttgccta tctcacccat gttcattctc tggtccttgc cgtcagcttc attgagcatc
     2101 ttcttgatgg caagcttaat aacatttata tccgtcattc ccttttcggt at
//
LOCUS       DASRQF010000039         2054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1091306, whole genome shotgun sequence.
ACCESSION   DASRQF010000039 DASRQF010000000
VERSION     DASRQF010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091306"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VFD79_01065"
     CDS             complement(<1..688)
                     /locus_tag="VFD79_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017551004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HZH92665.1"
                     /translation="MTKEKLTGISFFEKYLTIWVALCMAIGVAIGVYLPGVPEFLSQF
                     EYYSVSIPVAILIWLMIYPMMLKVDFKSVKKVGENPKGLIVTWVTNWLIKPFSMFAIA
                     YLFFNVIYSNTIDPEMAKQYLAGAVLLGAAPCTAMVFVWSHLTKGNPAYTLVQVATND
                     LIILVAFVPIVALLLGVSDILVPWNTLFLSVVLFVVIPLAAGVISRTVITKNKGLEYF
                     EKVYIPKFNNV"
     gene            complement(700..930)
                     /locus_tag="VFD79_01070"
     CDS             complement(700..930)
                     /locus_tag="VFD79_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZH92666.1"
                     /translation="MIIKVLGTGCKKCEKTEANVREAIKELGIEATIQKVEDVQGIMS
                     YGVMATPALVIDEEVKVKGKVPSVEDIKKMLD"
     gene            complement(968..1810)
                     /locus_tag="VFD79_01075"
     CDS             complement(968..1810)
                     /locus_tag="VFD79_01075"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZH92667.1"
                     /translation="MFEKIIIYSEPSSDKQDITEFIKGLRRLGGKECLLLYCLDPYEG
                     GAKVSSFYQKTLEKYAEKQKNILEGQGFQVETRVHIGEPDDEVNRLAKDKGYSLAAVG
                     GTTQSLLWEKFLGGMEYKIIHHSKIPTLVVRIRDFQDDDQLVQKVDITDHILFPTDFS
                     ENSMKAFDLILEMVGSGVENVSVLHVAEKETEEESIRDIMKRLQELEVDLVKAGAKKV
                     DVKILFGEPSDEIISYAEESDPTLVVMGTQGRGFLQEIFIGSVSHDMVRKSPVSVMLV
                     PADR"
BASE COUNT          533 a          515 c          385 g          621 t
ORIGIN      
        1 taacgttgtt gaattttgga atatatacct tctcaaagta ctcaagaccc ttgttctttg
       61 ttattaccgt ccttgagatt acaccagccg caagtggaat cacaacaaag aggaccactg
      121 aaagaaagag ggtattccaa ggtacaagaa tatcgctgac tccaagtaga agtgcaacaa
      181 tgggaacaaa ggcaacaagg attatcagat cgtttgttgc aacctggaca agtgtgtagg
      241 ccgggtttcc ctttgtcaga tgactccata caaacaccat tgccgtacat ggagcagctc
      301 caaggaggac agctccagcc agatattgct tggccatttc agggtctata gtattgctgt
      361 atatgacatt gaagaaaaga taggctattg caaacatgga aaagggcttt atgagccagt
      421 ttgtaaccca ggttacaatc agtcccttgg ggttttcccc aacctttttg acgctcttga
      481 aatcaacctt gagcatcatt ggatatatca tcagccagat aagtatggca actggtatag
      541 atacgctata gtattcaaat tgacttaaaa attccggtac tcctggcagg taaactccaa
      601 ttgcaacccc gattgccatg caaagggcaa cccatattgt aaggtacttc tcaaaaaagc
      661 ttattcccgt caatttttct tttgtcatct attatcctcc tagtctagca tcttcttaat
      721 atcttcaaca gacggaacct tgccctttac cttgacctct tcgtcaatta caagggcagg
      781 agttgccatt actccatagg acatgattcc ctggacatcc tcgaccttct ggatggtcgc
      841 ctcaattcca agctccttga tggcctccct tacatttgct tccgtctttt cacatttctt
      901 gcagcctgtt cccaaaacct ttattatcat aagtgattcc tccttgattt ttccttttgg
      961 tctcaatcta cctgtcagct gggacaagca tcactgaaac aggggatttc cggaccatgt
     1021 catggcttac gctgccaatg aaaatttcct gtagaaaccc tcttccctgg gttcccatga
     1081 ccacaagtgt tgggtctgat tcctcggcgt aacttatgat ttcatccgat ggctccccga
     1141 atagaatctt tacatccact tttttggccc cggccttgac caggtccact tcaagctcct
     1201 gcagcctttt catgatatcc cttatggact cctcctccgt ttccttttca gccacatgga
     1261 ggacgctgac attttcaacc ccagacccta ccatttcaag aatcaggtca aaggccttca
     1321 tcgagttctc cgaaaagtct gtcgggaaaa ggatgtggtc agttatgtcg accttctgga
     1381 ccagctggtc atcatcttgg aagtccctta tccttaccac aagtgtgggt atttttgaat
     1441 gatgaattat cttgtactcc atcccaccca ggaatttctc ccacaagagg gattgtgttg
     1501 ttccaccaac tgcagcaagg gaataaccct tatcctttgc aagcctgttt acctcatcat
     1561 caggctcccc gatatggacc cttgtttcca cctgaaagcc ctgaccttcc aggatgttct
     1621 tctgcttttc tgcgtatttc tccagggttt tctgatagaa ggaggatacc tttgctccac
     1681 cttcataggg gtcaaggcaa tacaatagca ggcattcctt ccctcccagc cttctaagtc
     1741 cctttatgaa ttcagttatg tcctgcttgt ctgatgatgg ctctgagtat attattatct
     1801 tttcaaacat atttagcccc cctcaactag acttgttcca agaaagtata tgtttagctt
     1861 ttcagatacc ttgcttcatt ggatatatac cctcttatgg ttgacttccc aatcaattgg
     1921 ccgattacaa tatgaaaaag tgtccaaatg cattgaatcc atatccaatt attagaatcc
     1981 cgatagtcac aataagcaag aatatggcaa gaagcttggt tttaaccacc ttcttgagca
     2041 ttatcaggga aggc
//
LOCUS       DASRQF010000040         2443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_846611, whole genome shotgun sequence.
ACCESSION   DASRQF010000040 DASRQF010000000
VERSION     DASRQF010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846611"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFD79_01080"
     CDS             <1..522
                     /locus_tag="VFD79_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HZH92668.1"
                     /translation="GSRIFFRNIFSPAVTSLATCSSIATLPVNLTATEKMNVPKDIRE
                     IVLPIGATMHMDGTALSSILKIAFLFGIFGQDFSGIGTYVTAVLIAVLGGVVMSGVPG
                     GGLIGEMLIVTIYGFPPEAFPIVATIGFLVDPPATMINASGDAVASMMVTRIVEGKNW
                     LGERVSKAVPSKA"
     gene            619..1599
                     /locus_tag="VFD79_01085"
     CDS             619..1599
                     /locus_tag="VFD79_01085"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HZH92669.1"
                     /translation="MKFIDLHCDTLMHTVGREEPLSLLRNDQTSIDFERMEEGGALAQ
                     FFAVFLPTEEMFLENVGEVIDDDLYINTLVDTLKREIGENSTMISLAHNHHDIIQNEK
                     EGRMSAILTIEDGRSVQGSLEKLRSYYDMGIRLISLTWNFENCFGYPNSDDRNIMEKG
                     LKEFGREAVEYMNQLGMIVDVSHLSDGGFYDVAKISKKPFVASHSNARDISPHKRNLT
                     DQMIRVLGEKGGVAGINFAPHFLQPDTTTDRSTVQLMVRHLNHMKNVGGEDVVALGSD
                     FDGIGGDLEVDSIEKMPMIFDALERDGWSQNQIEKLAYRNTLRVIKESMK"
     gene            complement(1643..>2443)
                     /gene="rbsB"
                     /locus_tag="VFD79_01090"
     CDS             complement(1643..>2443)
                     /gene="rbsB"
                     /locus_tag="VFD79_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004823267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HZH92670.1"
                     /translation="IGLVVSTLNNPFFVDLRDGAQAKADELGASLIVLDSQDDAATEL
                     SNVEDLVNQGVDLIMINPTDSDAVGSAVAAANGAGIPVVTLDRSANSGEVVAHIASDN
                     VAGGEMAGEFIVEQLGGAGKVVELEGIPGASAARERGEGFNAAIAGTDIEVVAKQTAN
                     FDRAEGLSVMENILQSQSEIDAVFAHNDEMALGALEAIRASGRDILIVGFDATDDAVA
                     AVEAGELAATVQQLPKDIGSIGVDTAMKVLGGEAVDEYIPVALELVTQ"
BASE COUNT          675 a          539 c          593 g          636 t
ORIGIN      
        1 ggaagcagaa tattcttcag gaatattttt agtccggcag tgacatcact tgcaacttgc
       61 agctcaattg caaccctgcc tgtaaacctt acggctacag aaaagatgaa tgtaccaaag
      121 gacattaggg agattgtact tccaataggt gcaacaatgc acatggacgg aacagccctt
      181 tcatcgatac ttaagatagc attcctcttt gggatattcg gccaggactt ttcaggaatt
      241 ggaacctacg taactgcagt gcttattgcg gttcttggag gtgtggtcat gtccggtgta
      301 ccagggggag gacttatcgg cgagatgctt atagtcacaa tctacggatt cccacctgag
      361 gcttttccaa tagttgcaac aataggattc ctggtggacc cacctgcaac aatgatcaat
      421 gcctctggag atgcggttgc atccatgatg gttacaagaa tcgtcgaggg caagaactgg
      481 cttggtgaga gagtctcaaa ggcagtccct tcgaaagcat aattgattat gtgatttcaa
      541 tgtggtaaag tatgttccga agggtattaa accttcggaa cattttttaa ttgaagaatt
      601 atggaggagg atgcttggat gaaattcatt gacctgcact gtgatacact tatgcatact
      661 gttggaaggg aagagccttt aagtctactt agaaacgacc agaccagcat cgattttgag
      721 cgtatggagg agggaggggc acttgcccag tttttcgctg tatttttgcc aacagaagaa
      781 atgtttctgg agaatgttgg agaggtcatt gatgatgatc tgtacataaa cacccttgtg
      841 gataccctga aacgggaaat aggtgagaac agcaccatga tctctctggc ccacaaccac
      901 cacgatatta ttcaaaatga aaaagaggga aggatgtctg cgatccttac catagaggac
      961 ggacggagcg tccagggaag ccttgaaaag ctaaggtcat attatgacat gggaataagg
     1021 cttataagcc ttacatggaa tttcgagaac tgctttggat accccaattc agatgacagg
     1081 aacataatgg aaaagggtct gaaggaattt ggaagggagg ctgttgagta catgaaccag
     1141 ctgggtatga ttgtggatgt gtcccacctt tccgatgggg gtttttatga cgtggcaaaa
     1201 ataagcaaaa agccctttgt ggcatcccac tcaaatgcca gggatatctc accccacaag
     1261 agaaacctta cagaccagat gataagagtc ctgggagaaa agggaggggt ggcaggaatc
     1321 aattttgcac ctcattttct ccagcctgac accacaaccg acaggagtac agtccagttg
     1381 atggtaagac accttaacca tatgaagaat gtgggagggg aggatgttgt ggccctgggg
     1441 tcggactttg atggcatagg cggagacctt gaggttgaca gcatagagaa gatgccaatg
     1501 atatttgatg cccttgaaag ggacgggtgg agtcaaaatc agatagagaa gctggcctac
     1561 aggaacacct taagggtaat caaggaatcc atgaaatgat taaagcacgg ccgaggccgt
     1621 gcttttttaa gtcaatgcta aactattggg taacaagctc aagggctaca gggatgtatt
     1681 catccactgc ttcgccgcca agaaccttca ttgctgtatc gacaccgatt gaaccgatgt
     1741 cctttggaag ctgctgtact gttgctgcca attctcctgc ctcaactgct gcaactgcat
     1801 catccgttgc gtcaaatcca acaatcagga tgtctctacc ggatgctctg atagcttcaa
     1861 gagctccaag tgccatttcg tcattgtgag caaatacagc atcgatttca gattgtgact
     1921 gaagaatatt ctccattact gaaagtccct ctgccctgtc aaagttggct gtttgttttg
     1981 caacaacctc aatgtcagtt cctgcaattg ctgcattgaa gccttcgcct ctttcccttg
     2041 cagctgatgc tccaggaata ccctccaatt caacaacctt gcctgctcca ccaagttgtt
     2101 caacgataaa ttcaccggcc atttctccac cggctacgtt gtctgaagcg atgtgagcta
     2161 caacttctcc tgagtttgct gacctgtcca gggtaaccac tggaatgcct gctccgtttg
     2221 ctgctgcaac tgcgctacct actgcatctg aatcagtcgg gttaatcatt attaggtcga
     2281 caccctgatt cacaaggtct tcaacgtttg acagctctgt agctgcgtca tcctgtgagt
     2341 caagaactat aaggctggct ccaagctcat cagcttttgc ctgagcaccg tctctaaggt
     2401 ctacaaagaa tgggttgttc aaggttgaaa ccaccaatcc tat
//
LOCUS       DASRQF010000041         2224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_843860, whole genome shotgun sequence.
ACCESSION   DASRQF010000041 DASRQF010000000
VERSION     DASRQF010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843860"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VFD79_01095"
     CDS             complement(<1..522)
                     /locus_tag="VFD79_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92671.1"
                     /translation="MNIKNIIAVGLVGASLALASPMSNAADMPQTIHEKIEKTPVSSG
                     VVHERIEKFTIGGWYNINVLRIDLRDEYTSLGPLYHQEGLSSRSTVKGLVEQRSAVAG
                     INGDFYNTNPIPTSLGALVENGRIISSPNNLPTFFTTMDNRAYIDYFNRTMTITNYNT
                     GNVLNIDTVNKLHS"
     gene            686..1864
                     /locus_tag="VFD79_01100"
     CDS             686..1864
                     /locus_tag="VFD79_01100"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HZH92672.1"
                     /translation="MVEIYTLGNFDIRLEDVSILKAVGNKQRLVKLFKYLLTFHGKKL
                     LPERIIEDLWQDEYTQPLRALRTQISRVRRMFPEEMWVSAAFFSIEYIDGYYVFNLHK
                     DCHVDFIQMQRCIDEVDCDGGYRDCEEIVHLYKGEYLGELGDEEWLVPVRNMFDRMYI
                     KSLSKYLQHLMDRGEYSKILTICEEAMRYKPYEESLHIFFMEALDALGETRYAINHYS
                     FCTTKLYRELGETPSDRMKDVYRRIKNGNGSNNNHKPMLQLNNIDCNINNSIDRIGAL
                     VCDTRQFEFIYGFEKLQMEREKMDLYLGILTLDQSGLVRNGEIDVEKSTKDFIGSIQY
                     NLRKGDVISQWNPNQALMLYKMDDECGANCLMDRIQKSFSRFDGSNKLSLNIKIKKIV
                     "
     gene            2037..>2224
                     /locus_tag="VFD79_01105"
     CDS             2037..>2224
                     /locus_tag="VFD79_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92673.1"
                     /translation="MKIINSKVFVMIVVMAMVLSIGTTSLGEEIDLEELVAEETVVEE
                     IMESTVIEDPVTDEAQGE"
BASE COUNT          661 a          361 c          543 g          659 t
ORIGIN      
        1 tgaatggagc ttgttgacag tatcgatgtt aaggacattt cccgtgttgt agttggttat
       61 tgtcattgtc ctgttgaagt agtctatata tgccctgtta tccatagtgg tgaagaaggt
      121 tggaagattg ttaggagagg atatgattct tccattctcc acaagggcac caagtgatgt
      181 tggaattgga ttggtgttgt agaaatctcc attgattccg gcaacagcac ttctttgttc
      241 aacaagtccc tttacggtac tgcgggatga gagcccctcc tggtggtaca agggtcctaa
      301 tgatgtgtat tcatccctca ggtcgattct aagtacattt atattgtacc agccacctat
      361 ggtaaacttt tctatccttt cgtggacaac ccctgaggaa accggagttt tttctatctt
      421 ctcgtgaatt gtttgtggca tgtctgccgc atttgacatt ggacttgcaa gtgccagtga
      481 ggctccaaca agacccacag ctataatatt ttttatgttc atggaatgtc tcctttcatt
      541 ttgcatcgtt aggtctatta tatagtttgc atggtacagg taggttacaa acctgttacg
      601 aatatattaa atgtttcggg cttaaattaa tgacatatgt ttaataattc acaagaaggg
      661 tatattttac ctagaggtgt ggggtatggt cgaaatatat accttgggta atttcgacat
      721 tagacttgaa gacgtatcta ttttgaaggc tgtaggaaac aagcaaaggt tggttaaatt
      781 attcaagtac ctattgacct ttcacggaaa aaaactgctc cctgaaagga ttattgaaga
      841 cttatggcag gatgaataca cacagcccct aagggcgcta agaactcaaa tctcaagggt
      901 gagaaggatg ttccctgaag agatgtgggt ttctgcggcc ttttttagca ttgagtacat
      961 tgatggatac tatgtattca atctccacaa ggactgccat gttgacttca tccaaatgca
     1021 aaggtgtatt gatgaggttg attgtgatgg aggctacagg gactgcgagg aaatagttca
     1081 tctctacaag ggtgagtatc tgggagagtt gggcgatgag gagtggcttg tgcctgtaag
     1141 gaatatgttt gataggatgt atatcaagag tctatcaaaa tatttgcagc atctaatgga
     1201 taggggtgaa tacagtaaaa tattgacaat ctgtgaggag gcaatgagat ataagcctta
     1261 tgaggagtca ctacatattt tctttatgga agccttggat gccttgggtg agacaaggta
     1321 tgccatcaac cactacagct tttgcaccac caagctctac agggagctgg gggaaactcc
     1381 aagtgacagg atgaaggatg tctacaggag gatcaagaac ggaaacggca gcaataacaa
     1441 tcacaagcca atgcttcagt taaataacat tgactgcaat atcaacaatt ccattgatag
     1501 gattggtgcc ctggtctgtg atacacggca gtttgagttt atctatggat ttgagaagtt
     1561 gcagatggaa agggaaaaga tggatctgta ccttgggatc ttgacccttg atcaatctgg
     1621 attggtaagg aacggcgaaa tagatgttga aaaaagcacc aaggacttta ttggaagcat
     1681 tcaatataat cttaggaaag gtgatgttat atcccaatgg aatccaaacc aggcattgat
     1741 gctatataag atggatgacg aatgtggagc gaattgcctg atggatcgga ttcagaaaag
     1801 cttttccagg ttcgatggat ccaataaact atctctaaac ataaaaatca aaaaaattgt
     1861 ttaatatttt ttaggctaag aatccctgtt ttaaaggcaa tgggggttct ttttttgtct
     1921 tgaaatcaca tgaaatccat ctgtaatcat gctgaaacaa acctgtaatt gagacaggta
     1981 tatattcagt ggaacgagat attacaaaat gattacgaca atggaggatg atggccatga
     2041 agattattaa tagcaaggta tttgtgatga tagtggtaat ggccatggtg ctttccattg
     2101 gaaccacctc attgggtgag gaaattgatc tggaggaact tgttgctgag gagactgttg
     2161 ttgaggaaat tatggagtct acagtgatag aggacccggt aacggatgaa gctcaggggg
     2221 aaga
//
LOCUS       DASRQF010000042         2248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1337617, whole genome shotgun sequence.
ACCESSION   DASRQF010000042 DASRQF010000000
VERSION     DASRQF010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337617"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..1990
                     /locus_tag="VFD79_01110"
     CDS             278..1990
                     /locus_tag="VFD79_01110"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HZH92674.1"
                     /translation="MRLKVSESKNAASYYVIKDYYSNGKRTTKIVEKLGTHDELLEKL
                     GGKDPVQWAKEYIEELNKKEKDEKNDVLVRYSPSKQINLGQQRLFNGGYLFLQKIYHQ
                     LGIQNISESISKKYRFAFNLDSVLSRLVYGRIIFPSSKLATYDLSSRFIEQPDFDIQH
                     VYRALDVISKETDFIQSELYKNSLKISKRNTGVLYYDCTNYFFEIEEESGLREYGHSK
                     DHKPNPIVQMGLFMDGDGIPLAFDIHSGRTNEQKTLKPLEKKILYDFKLSRFVVCTDA
                     GLSSIENRKFNDMGSRAFITTQSVKMLKGFLKEWALDPKGWMYGDSGKTYDISKLDEE
                     KDYSKTFYKERWINENGLEQRLIVTFSLKYRNYKMQIRENQIQRAVDVIEKNPAKIKK
                     SGSNDFKRFIEKTNVTTDGEVAEKELFSINKTLISKEAMYDGFYAVCTNLEDDISAII
                     SVNHRRWEIEECFRIMKSEFKSRPVYLSRDERIKAHFTTCFLSLVLYRYLEKALDGKF
                     TTCEIIKGLRDMNFYEIPGEGLIPTYVRNDFTDALHDAFGFRTDYQILKLKQVKNILK
                     ITKS"
BASE COUNT          721 a          379 c          503 g          645 t
ORIGIN      
        1 attgcctgat gttaaaaaga tggacctgga ctggtcatcc cacatcacat caaaatattt
       61 tggtgtagat ttcagagcat atgcaatatc tctaagctta aagtagctag tattgtcgat
      121 taagtatgac cctattgata ccgggagttt gacagtttaa caaagatata acccttgaaa
      181 gccttgaaat tatcggcttt ttattatttt tttgtcattt ttacattgcg taccttttcg
      241 ccatgtgata taatagtact atatgggagg ggtttttatg aggttgaaag tcagtgaatc
      301 aaagaatgct gcttcatatt atgttattaa ggactattac tccaatggaa aaagaacaac
      361 taagattgta gaaaagcttg gtactcatga tgagcttctt gagaagttag gtggcaagga
      421 tcctgtccag tgggccaaag agtatatcga ggaattgaac aagaaagaga aggatgaaaa
      481 aaatgatgtt ctggtaaggt attctccttc taaacagata aatctagggc aacaaaggct
      541 ttttaacggc ggatacctgt ttcttcagaa aatataccat cagttgggta tccaaaacat
      601 ctctgagtcc atatccaaaa agtacagatt tgcctttaat ctggactctg tgctttctag
      661 gctcgtgtat gggagaatca ttttcccatc ttctaagctt gccacctatg atctctcttc
      721 cagattcatt gagcagcctg attttgatat tcagcatgta tacagggctc ttgatgtgat
      781 ttcaaaggaa actgatttta tccaatctga actatacaag aatagtttaa agatttccaa
      841 gaggaacact ggggtccttt attatgattg caccaattat ttctttgaga ttgaggagga
      901 gtctgggtta agggaatatg gtcattccaa ggaccacaaa ccaaacccca ttgtccagat
      961 gggtctgttt atggatgggg atggaatccc tcttgccttt gacatacata gcggaaggac
     1021 caatgaacaa aaaactctta agcctttgga gaaaaagatt ctctatgatt tcaagctttc
     1081 aaggtttgtt gtttgcacag acgctggtct ttcatccatt gaaaacagaa agttcaacga
     1141 tatgggcagc agggccttta tcaccaccca atctgtaaag atgcttaagg gttttctcaa
     1201 ggagtgggct cttgacccca agggatggat gtatggggat agcgggaaga catatgatat
     1261 ttccaaactg gatgaagaaa aggactactc gaagaccttc tacaaggaaa gatggatcaa
     1321 tgagaatggt ctggagcaaa ggctgattgt aaccttttcg cttaaataca gaaactacaa
     1381 gatgcagatt agagaaaacc agatacaaag ggctgtggat gtgattgaaa agaatccagc
     1441 gaagattaag aagtccggtt ccaatgattt caagaggttt atcgagaaga ccaatgtgac
     1501 cactgatggt gaagttgctg agaaggaatt gtttagcata aacaagactc tgatttccaa
     1561 ggaagccatg tatgacgggt tctatgctgt atgtacaaat ttggaggatg atatcagtgc
     1621 cataatcagc gtcaatcata gaaggtggga aatcgaggaa tgcttcagaa tcatgaaaag
     1681 tgaattcaag tcaagaccgg tctacctaag tcgagatgag aggatcaagg ctcactttac
     1741 aacttgcttc ttgtccttgg ttttgtacag gtaccttgag aaggcccttg atggcaagtt
     1801 tacaacctgt gagataatta agggactaag ggacatgaac ttttatgaga ttcctggaga
     1861 aggccttatt ccaacctatg tgaggaacga cttcaccgat gccctgcacg atgcatttgg
     1921 ctttaggact gactatcaga tactaaaact gaaacaagtc aaaaatattt taaaaatcac
     1981 gaaaagctaa aaaaggtacg cacttttcga gtaatatgaa aaagcttaga acgttgttat
     2041 tgcaacatgt tccaagcttt ttttgctttt taactgtcaa atatgggaat gtattgtaac
     2101 actcaagaag aaaactaaca accggtattg aacggtttat gaatattgaa aggcggggag
     2161 gtaatcctat gggctttata agcgaatggc aggataatta tgtggtgaag caggtgaaaa
     2221 acctgaagct accagagttt ggtgaatc
//
LOCUS       DASRQF010000043         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1457207, whole genome shotgun sequence.
ACCESSION   DASRQF010000043 DASRQF010000000
VERSION     DASRQF010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457207"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(335..1309)
                     /locus_tag="VFD79_01115"
     CDS             complement(335..1309)
                     /locus_tag="VFD79_01115"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZH92675.1"
                     /translation="MKRMLALLMAGALIVGLMGGCARESAARGGNYKIAIVQPVDHPS
                     LNQIRDSIRDELVVLGLEDNFEISFHNANGDMNMLQSIMQNLVGDEVDMIVPIATPTA
                     QAALASTKDIPIVFSAVSNPVEAGLVEDLSETPGNITGVSDSIPVEDIMKLSHVLTPE
                     VKTYGLIYNSSEINSVTGIEKAKTYCEENGLDFREGTITSTSELHQVVSSLVGEVDAF
                     FTPDDNMVASAMPTYVKLAIEAGLPIYVGADSMVNDGGFATVGIDYTLLGKQTGRMIA
                     RIMDGESIGENPVEQMAEYSNMINMDTAKDLEIDIPKELLDEFILIYK"
     gene            complement(1723..>2102)
                     /locus_tag="VFD79_01120"
     CDS             complement(1723..>2102)
                     /locus_tag="VFD79_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018213997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HZH92676.1"
                     /translation="KFNNVTIGGLLLTLIIIFSFQGQIIIENPMNILLIAIPLIIQTF
                     LIFFIAYMWAKAWKLPHNVAAPAGMIGASNFFELAVAVAISVFGLTSGAAVATVVGVL
                     VEVPVMLILVKIANNTRGWFRTA"
BASE COUNT          516 a          520 c          433 g          633 t
ORIGIN      
        1 ccttttatgt tttttgtatt ggcatcaggc aggtatatat acaaaaaatc tgcccttaaa
       61 ataagggcag attataatct gctataccac cttatgttta tattgaatct agaatgccaa
      121 cacattcctt gccgttaatg ccggcttaca gcttggatac tcgcacctgt ctaaacaggg
      181 ctttcgcctc gccctcagtg gggaatttac cgaacagtat ctccgcagga tttccaccat
      241 cacctgctct ctattggcgc ttgattcgat ttgacttcca cgtcatcggt ttgttgtatt
      301 agattatatg ggctgaaatt atcaatgtca agaattattt gtaaattaaa ataaattcat
      361 ccaaaagttc ctttggaatg tctatttcaa ggtcttttgc tgtgtccata ttaatcatgt
      421 tggagtactc tgccatctgt tccacaggat tctcccctat ggactctcca tccataattc
      481 tcgcaatcat cctcccagtc tgcttcccaa gaagggtgta gtcgattccc acagtggcaa
      541 atcccccgtc atttaccatg gagtccgctc caacatagat tggcagtcca gcctctattg
      601 ccaatttcac ataggttggc attgcagatg ccaccatatt gtcatccggt gtgaagaatg
      661 catccacttc tccaacaagt gaagatacta cctgatgcag ctcggatgtg gatgttatgg
      721 ttccctccct gaaatccagg ccattttcct cgcagtaggt ctttgccttt tcaattcctg
      781 taaccgagtt tatttcgctg ctgttataga tcaggccata ggtttttact tctggagtca
      841 ggacatgtga tagtttcatt atatcctcca ctggaattga atcggatacg ccagttatgt
      901 ttccaggggt ttcagacagg tcttccacaa gccctgcctc cacagggttg ctcaccgctg
      961 agaagactat gggaatgtcc tttgtcgaag ccagggctgc ctgggctgtg ggtgtcgcta
     1021 tggggacgat catgtcaacc tcatccccaa caaggttttg catgatggac tgaagcatat
     1081 tcatgtctcc atttgcgttg tggaatgaga tttcaaagtt atcctcaagt ccaaggacta
     1141 ccaactcatc ccttatcgag tccctgatct ggttcaggga tggatggtca acaggctgaa
     1201 caattgcaat tttgtagttt cctcctcttg cggccgattc ccttgcgcac ccacccatca
     1261 gtcctacgat taatgctcct gccataagta atgccaacat ccttttcatc ttgcacctct
     1321 ccttttgttt tgttagattt tttgtattgg cactcaggca ggctcctgca aaaaacaaaa
     1381 aactgtcctc aaaaataagg acagatcaga tctgctatgc caccttaagt attgtaatgg
     1441 accgctccca tagttcacgg agcgattttc atcctggaat gccaacacat cccttgccgt
     1501 taatgccggc ctacagcttg gatactcgaa actttctttc acctcgccct cagtgggcca
     1561 tttgccgaac agcatctccg caggatttcc accatcacct gctctctttt ggcgcttgat
     1621 tcggtttgat ttccacttca tcggtttgtt gttatagatt atatggcttt gatattcact
     1681 tgtcaaggat aaaatttaac cctcccgaag gagggccatc cattaggctg tcctaaacca
     1741 gcctcttgtg ttgttggcaa tctttacaag tatgagcatt acaggaacct ccacaaggac
     1801 accaacaact gttgctacag cagctccgga tgtcaggccg aatacagaga ttgcaacagc
     1861 cactgccaat tcaaagaagt ttgaggcccc gatcattccc gcaggtgctg caacattgtg
     1921 gggaagcttc catgcctttg cccacatgta ggcaatgaag aagatcagga aggtctggat
     1981 tataaggggt attgctatta ggagtatatt catgggattc tcgattatga tctgcccctg
     2041 gaaggagaag atgattatca gggtcaggag cagccctcca atagtaacat tgttgaactt
     2101 tg
//
LOCUS       DASRQF010000044         2015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_589841, whole genome shotgun sequence.
ACCESSION   DASRQF010000044 DASRQF010000000
VERSION     DASRQF010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589841"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VFD79_01125"
     CDS             complement(<1..717)
                     /locus_tag="VFD79_01125"
                     /inference="COORDINATES: protein motif:HMM:NF013885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HZH92677.1"
                     /translation="MFKILAHIKKKLVIYLPVTMILAVVYGYFIDPAPLRSLVPVIVF
                     FMVYPMMVTLRYKELLSKGNTKLHLVAQGVNFIIFPVMGYVIGRVFFSDNPYIMIGLL
                     LMALLPTSGMTISWTGFAKGNVSAAIKMMVIGLILGSVLMPLYLKLFLGETAGIPFSS
                     IINEIVKVVFIPMALGFGTQKFLLRKFGKDRFQNDFKKKIPLIATAALVGIVFISTAM
                     RAKVIVADPSMMVRIFLVLSV"
     gene            complement(834..1961)
                     /locus_tag="VFD79_01130"
     CDS             complement(834..1961)
                     /locus_tag="VFD79_01130"
                     /inference="COORDINATES: protein motif:HMM:NF005593.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HZH92678.1"
                     /translation="MKIKPFKVEQWMNDYEMDAIYNIAETCVDAMSLSELIQLSGEDK
                     DSLLDDILDMKMTYGYITGNPRLKDAISSLYKGIGRDHILTTNGGIGANNLVLTTIVE
                     PGDKVVSIIPTYQQLYSIPESLGADVHIMYLQKEKNFLPDMEKLKEMVDADTKIICLN
                     NPNNPTGALIPDHMMEEIVDLAKSVDAYILCDEVYRGLNQDGSYHKSIVELYDKGIGT
                     SSMSKVFSLAGLRLGWLVTRDKELMERLFSHRDYSIISCGVVDEVFGALALENKDKVL
                     DRNLKIIRDNLRILDDWIKTHERFSYVKPIAGTTALVFYDYDISAFEFCTGLMEHSKT
                     FVTPGSCFEYENSFRIGYAASKETLVEGLKNLEEFCRGLEK"
BASE COUNT          538 a          475 c          377 g          625 t
ORIGIN      
        1 aactgaaagt accagaaaaa tcctgaccat catggatgga tctgccacaa ttaccttggc
       61 tctcatggcc gtggagataa agacaatccc aacaagtgct gctgttgcaa tcaaggggat
      121 ctttttcttg aaatcattct ggaatctgtc cttcccaaat ttcctgagca gaaacttttg
      181 ggttccaaat ccaagagcca taggtatgaa tacaaccttc acaatctcat tgatgatgct
      241 ggaaaatggt attcctgcag tttcccccaa aaatagtttc aaatagagtg gcataagcac
      301 cgaccccaat atgagtccta taaccatcat cttgatggct gcagagacat ttcccttggc
      361 aaagccagtc caggaaattg tcattcccga agttggaagc agagccatca agagcagacc
      421 tatcattata tatgggttgt cggaaaagaa gactctacca attacatatc ccattaccgg
      481 gaaaataatg aaattcacac cctgggctac caggtgcagc ttcgtatttc cctttgagag
      541 tagttccttg tatcttagtg tgaccatcat tggataaacc ataaagaaga caatcactgg
      601 tacaagtgat ctaagtggtg caggatctat gaagtatccg tagaccaccg ccagaatcat
      661 tgttacgggt aagtagataa ccagcttctt ttttatatgt gcaaggattt taaacatatg
      721 aattcctcct atatctttta tacccctaag gggtatcttt ctatatggta taccctcctg
      781 acatttttgt caagaaaaaa tgccggagtt ctccggcatt agaattcaag tatttacttt
      841 tccagtcccc tgcagaattc ctccaggttt ttcaaaccct caacaagtgt ttcctttgaa
      901 gcggcatatc caatcctgaa gctattttca tattcaaaac aggatccagg tgttacaaat
      961 gtttttgaat gttccatcag tcctgtgcaa aactcgaatg cactaatgtc atagtcgtaa
     1021 aataccagtg cagtggttcc tgcaatgggc ttcacgtatg aaaatctttc atgggtttta
     1081 atccaatcat ccaatattct aaggttatcc cttatgatct ttaggtttct atcaaggacc
     1141 ttgtccttgt tttccagggc cagtgcaccg aatacctcat ccaccactcc acagcttatg
     1201 atgctgtaat ccctgtgact gaacagcctt tccatcagtt ccttatccct tgtgacaagc
     1261 catcccaatc tcaagcctgc cagggagaat acctttgaca tgctgcttgt gccgatcccc
     1321 ttgtcataaa gctccacaat cgacttgtgg tagctgccat cctgattaag ccccctgtac
     1381 acctcatcgc atagtatgta tgcatccact gacttggcaa ggtctacaat ctcctccatc
     1441 atatgatcgg gaataagggc tccggtcgga ttattgggat tgttaagaca aattatcttg
     1501 gtgtccgcat ccaccatttc cttgagcttt tccatgtccg gcaaaaaatt cttctctttt
     1561 tgcaggtaca ttatatggac atccgcaccc agtgactctg gtattgagta aagctgctgg
     1621 taggttggta tgattgatac caccttgtct ccaggttcca caatggtggt tagcacaaga
     1681 ttgttggcac cgataccacc attggtcgtt agaatgtggt cccgtcctat tcccttgtat
     1741 agggatgata ttgcatcctt aagtctggga ttgcctgtta tgtatccata ggtcatcttc
     1801 atgtccagga tatcatcgag aagactgtcc ttgtcctctc ctgacagttg gatcagttct
     1861 gaaagggaca ttgcatccac acatgtttcg gctatattat atattgcatc catttcataa
     1921 tcattcatcc actgctcaac cttgaatggt tttattttca tagtccttcc tccaatcatt
     1981 ctttgtttag gttgcctatt ttttgattat atcat
//
LOCUS       DASRQF010000045         4814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1086903, whole genome shotgun sequence.
ACCESSION   DASRQF010000045 DASRQF010000000
VERSION     DASRQF010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086903"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..719)
                     /locus_tag="VFD79_01135"
     CDS             complement(45..719)
                     /locus_tag="VFD79_01135"
                     /inference="COORDINATES: protein motif:HMM:NF017655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HZH92679.1"
                     /translation="MIKLNGLLLKSVALITMIIDHYGAIFQPTEMTFRMIGRLAFPIY
                     CFLLVEGFIHTRNIKRYALRLLVFALISEIPFDYAFYRGLNWGHQNIFFTLLIGLIAM
                     YFIERKDVQESATPLIAVGAAAAATFLATDYSFIGIIYILVFYYTRNIPAIRRMSLAA
                     GIIFLTNLVSTGWIQQYSLLSLPILLTYNNKPGPKIKPLQYSFYLAYPAHLALFAAII
                     LGYLSF"
     gene            830..1057
                     /locus_tag="VFD79_01140"
     CDS             830..1057
                     /locus_tag="VFD79_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009372535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PC4/YdbC family ssDNA-binding protein"
                     /protein_id="HZH92680.1"
                     /translation="MAEIKFEITKKLGALSESQRSWKKEINMVSWNNYPAKYDIREWD
                     PDHEKMGKGVTFNKDEIIALRNILNQLDLED"
     gene            complement(1092..1424)
                     /locus_tag="VFD79_01145"
     CDS             complement(1092..1424)
                     /locus_tag="VFD79_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92681.1"
                     /translation="MSYVVSMILNLTIVILVVFTQLHLYKSNEHMSKCLTCSFSLTMA
                     VLLAMLNFSTSSGTNSFEGIFALYPIMIALFAFYLRWKKERLTMFIRFIFSVAVLAGL
                     ADYLTYSL"
     gene            1578..3068
                     /locus_tag="VFD79_01150"
     CDS             1578..3068
                     /locus_tag="VFD79_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZH92682.1"
                     /translation="MDELNNYYIKTNRTFTTIRKYAWIFTFLVAIGGLWEPRLGVLVV
                     GIMAGLLGTSFFSGRHWCGNFCPHGSLFDNIINPISMNKKIPAFLKSKWFVGAFFLFF
                     TINFSRKLFRIAAFWGLYSFIERLGYIFVNTYLMVLVVGGAVALLVNSRTWCQFCPMG
                     TLQKLSYRLGKAVGVVKHTEKRITIESKDKCHTCGKCARVCPFQLTPYLEFNENNQFD
                     NINCIKCTTCVENCPANLLSLETEHKAVNIVEMSDIKGYENRQEIESTLIEINDLGGD
                     LREFVFRFENPREVHYKAGQFILVKIQDKPVQYRAYSISSANDDNRILKVIIKKVANG
                     YGTEIIFRFKVGDKVILEGPMGNHLVPSEDTKKMLFIANGIGITPFIGLAKENLTKGE
                     AFESVNLLNGQRYVDDFIYHDYFTDLQKEYNNFEYLPAASREDHEGFHKGYVTELLKT
                     MDVTGHKVYMCGAGAMIRECYNILRSKGVPSGDIFFESEERIKLSA"
     gene            3144..3551
                     /locus_tag="VFD79_01155"
     CDS             3144..3551
                     /locus_tag="VFD79_01155"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HZH92683.1"
                     /translation="MLYFAYASNLSKKYMSSRCPYAIPLKKVSLKGYKLTFNELADIV
                     PTEGEDVVGGLYLISKQELEKLDILEGHPDLYERIIVEVVDDDGNLYEAFVYSMVDKK
                     EQAPPDSYYNILKDGYEDWDIDMERLEKARSFE"
     gene            3614..4117
                     /locus_tag="VFD79_01160"
     CDS             3614..4117
                     /locus_tag="VFD79_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HZH92684.1"
                     /translation="MLKNLGEIKPQIHENAFIAETASVIGDVKVGEGSSVWYGAVIRG
                     DIEDIQIGKYSNIQDNASVHTETDIPTRIGDYTVVGHNAIIHGCTLGNNTLVGMGAII
                     LNNAEIGNNCIIGAGSLVTEGKKIPDNSMVMGIPGKVVREVTEEEIKGIRENAVRYNT
                     LYKKHLD"
     gene            4227..4793
                     /locus_tag="VFD79_01165"
     CDS             4227..4793
                     /locus_tag="VFD79_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZH92685.1"
                     /translation="MNNNRTRMSREDRKAQIIQVAVELFVEQGYKGTTTAQIAEAAGI
                     SEVTLFRNFDSKREIFLQGVMPILTETLMDRLKVDENISREEALERFLKDRLAVISEY
                     HGVVRLVLMENQMSHEFKDIDFIKQIGGQIQRGLHEMGFKTAKKDFTIRLIMGSILSF
                     LYLPEKDDNTVKEFAAELARAISLIDNN"
BASE COUNT         1609 a          785 c         1115 g         1305 t
ORIGIN      
        1 ggcgatgata tcctttttct acagccaatc ggcggaggat gattctagaa actcaaatat
       61 cccaatatga tagctgcaaa aagagccaga tgtgcaggat atgccaaata gaaggaatat
      121 tgcagaggct ttatttttgg gccgggttta ttgttgtagg tcaagagtat gggcagtgac
      181 aggagagaat attgttgaat ccatccagtt gaaaccagat tggttagaaa tatgatccca
      241 gctgccaggg acatccgtct tatggctgga atatttctgg tgtaatagaa gacaaggata
      301 tatataattc caataaagct gtagtctgta gccaggaagg ttgctgcagc tgcagcccca
      361 actgctataa gtggagtggc gctttcttga acatcctttc tttctatgaa atacattgca
      421 atgagtccga taaggagggt gaagaaaata ttctgatgcc cccagttaag acccctgtaa
      481 aatgcataat caaatggtat ttccgagatt aatgcgaaaa caagcaatcg cagggcatac
      541 ctttttatgt ttcttgtatg tataaatcct tcgacaagta gaaaacagta gattgggaat
      601 gccagccttc caatcattct aaaagtcatc tccgttggtt ggaaaattgc accatagtgg
      661 tcaattatca ttgttataag tgcaacagat tttaagagaa gtccgttcaa tttgatcacc
      721 gctttcttag attaattaga ttataacaaa aagcttaaaa agattataca tgataggata
      781 aatacctgtt ataatggtca gagagatata ctatacagga ggtaagtcaa tggcggaaat
      841 caaatttgaa ataacaaaga aactgggagc cctaagtgag tctcaaagaa gttggaaaaa
      901 ggaaataaat atggtttcgt ggaataatta ccctgccaag tatgatatta gagaatggga
      961 tcctgaccat gaaaagatgg gaaaaggtgt tacattcaac aaggatgaaa taatagcatt
     1021 aaggaatatt cttaaccaac tggatctgga agactaaaaa accactgtat caaatccttg
     1081 atacagtggt tttataatga atatgtaaga taatcagcca acccggctaa aacagccact
     1141 gagaaaatga acctgataaa cattgtcaaa cgttcctttt tccatctaag gtagaaggca
     1201 aacaaggcta tcattattgg ataaagcgcg aatatccctt caaatgagtt tgttccagat
     1261 gacgttgaaa aattcaacat ggcaagcaag acagccattg tcagactgaa ggaacatgtc
     1321 aaacacttgc tcatgtgctc attgctctta taaaggtgaa gctgtgtgaa aacaaccaaa
     1381 ataacaattg taagattgag gatcattgaa acgacgtaac tcaaagaaat ccctccttca
     1441 ataccattat accaatacaa atatatatgt aaaccctaaa atgacaatat gttaacaaac
     1501 attgacaaag gattgatccc ctagtataat tataaactgt acccaggcag ggtataacac
     1561 gaaaaggggg atttgacatg gatgaattga ataattacta cataaaaaca aatagaactt
     1621 tcaccacaat aagaaaatat gcttggattt ttacatttct tgtggcgata ggaggtctgt
     1681 gggaacctag attaggggtc ctggtggtgg gcataatggc tggtcttttg ggaacatcat
     1741 ttttttcagg taggcattgg tgtggaaatt tctgtcccca tggcagcctt tttgacaaca
     1801 taataaatcc catcagtatg aacaagaaga taccagcatt tctaaagagc aagtggtttg
     1861 ttggagcatt cttcctcttc ttcacaatca acttttcaag gaagctgttt agaattgcag
     1921 ctttttgggg attatacagt tttattgaga ggctgggata tatctttgtt aatacatacc
     1981 tgatggtctt ggttgttgga ggagcggttg cccttcttgt taattcaaga acttggtgtc
     2041 agttctgccc aatgggaact cttcagaagc tgtcatacag actgggaaag gctgttgggg
     2101 tcgtaaagca tacggaaaag agaattacaa tagagtcaaa ggataagtgc cacacctgcg
     2161 gcaagtgtgc tagggtttgt ccattccagc taacccctta tctggagttt aacgagaaca
     2221 atcagtttga caatatcaac tgcattaaat gtaccacttg tgttgaaaat tgtcctgcca
     2281 atctcttaag cctggaaaca gagcacaagg cagtaaatat agtcgagatg agcgacatta
     2341 aaggttatga gaacaggcag gaaattgaat ccactctaat tgaaatcaat gaccttggtg
     2401 gagacctgag ggaatttgtt ttcagatttg agaatccaag ggaggttcat tacaaggcgg
     2461 gtcagtttat ccttgtgaaa atacaggata agccagttca gtacagagcc tactccatat
     2521 cttcagccaa tgatgataac agaatcctaa aggtgatcat caagaaggtg gcaaatggat
     2581 atggtactga aatcatattc agattcaagg ttggagacaa ggttattctg gaggggccca
     2641 tgggaaatca cctggtacct tctgaggata ccaaaaagat gctgtttatc gccaatggta
     2701 tcggaataac accttttata ggtcttgcca aggagaatct tacaaagggt gaagcctttg
     2761 aatctgttaa cttgctaaac ggtcagaggt atgttgatga tttcatttat catgactatt
     2821 tcactgacct tcaaaaggag tacaacaatt ttgagtatct gccggctgca tcaagggaag
     2881 accatgaagg cttccataag ggctatgtga cagaattgct caagaccatg gatgtaaccg
     2941 gccataaggt atacatgtgc ggcgcaggtg caatgatcag ggagtgctac aacatattaa
     3001 ggtccaaggg agttccttct ggggatatat tctttgaaag tgaagaaaga attaagctta
     3061 gtgcatagat aatagaaata gatgcgaata taatgcacct ccaaggggta atagaatagt
     3121 gaggattccg aggaggtgtt tttatgttgt attttgctta tgcaagcaat ctaagcaaga
     3181 agtatatgtc cagtagatgt ccatatgcaa taccattgaa aaaggtaagc ctaaagggct
     3241 acaagcttac cttcaatgag ttggcggaca tagttccaac tgagggggag gatgtagtag
     3301 gaggtcttta cctcatatca aaacaggaac ttgaaaaact ggacattctt gagggacatc
     3361 ccgatcttta tgaaaggata atcgttgaag ttgtagatga cgatggcaac ctgtacgagg
     3421 cctttgtgta ttcaatggta gacaagaagg agcaggctcc gcctgacagc tactacaata
     3481 tactcaagga tggctacgaa gattgggata ttgacatgga aagacttgaa aaagccagaa
     3541 gttttgaata acaatcatcc cccggtcctg gactggggga acttttaaat attaaagata
     3601 ggggagtgct tttatgttaa aaaatttagg tgaaatcaaa ccacagattc atgagaatgc
     3661 ctttatagcg gagacggctt ctgtgatagg tgacgttaag gtaggggaag gaagcagcgt
     3721 ctggtatggt gctgttatca ggggagatat cgaagatatc caaattggga aatactcaaa
     3781 cattcaggac aatgcttccg tgcacacgga aacagatatc ccaaccagaa taggtgacta
     3841 tacagttgtt ggtcacaatg caataataca tggttgtacc ttgggaaaca ataccctggt
     3901 gggtatgggt gcaattattc ttaacaatgc tgaaattggg aataattgca taataggtgc
     3961 aggatccctt gtaacggaag gcaagaaaat tcctgataat tcaatggtta tggggatacc
     4021 aggaaaggtt gtaagagagg ttacagaaga ggaaataaag ggtatcaggg aaaatgcagt
     4081 taggtacaac accctgtata aaaagcattt ggattagaaa ttttaaataa acccattaag
     4141 ccggagagaa atcttcggtt ttttctttga caaaaatcct atttgtggta tcataagtaa
     4201 gtaagcactt acataaggag gtaattatga ataacaatag gactagaatg agtagggaag
     4261 atagaaaagc tcaaataatc caggtagcgg tagaactctt tgtggaacaa gggtacaagg
     4321 gaacaacaac agctcaaatt gccgaggctg ctggaatttc agaggttaca ctatttagga
     4381 attttgattc taaaagagaa atttttctgc aaggggtgat gccaattctt actgaaaccc
     4441 ttatggatag gcttaaggta gatgagaata tcagtcgtga ggaggctttg gaaagattcc
     4501 taaaggacag gttagctgta atttccgaat atcatggagt ggtgagactt gtgctcatgg
     4561 agaaccagat gagtcatgag tttaaagaca tagacttcat aaagcagatt ggaggtcaaa
     4621 ttcaaagggg ccttcatgaa atgggattca aaacagctaa gaaggacttt acaataaggc
     4681 ttataatggg tagcattcta tcattcctct atctgccaga gaaggatgat aatactgtta
     4741 aggagtttgc agctgaattg gcaagggcaa tcagtttgat tgacaataat tgataggggt
     4801 ggttggatga gtat
//
LOCUS       DASRQF010000046         3114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1455987, whole genome shotgun sequence.
ACCESSION   DASRQF010000046 DASRQF010000000
VERSION     DASRQF010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455987"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..301)
                     /locus_tag="VFD79_01170"
     CDS             complement(137..301)
                     /locus_tag="VFD79_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HZH92686.1"
                     /translation="MLRWSVIFIVVALVAAVLGFGGIAGAATEIAKILFFVFLVLFVL
                     SLIFGKKLNP"
     gene            complement(401..826)
                     /locus_tag="VFD79_01175"
     CDS             complement(401..826)
                     /locus_tag="VFD79_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019116747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HZH92687.1"
                     /translation="MLSVNISEKKGVVKEPVDQGEFKVDHGLVNDAHSGDWHRQVSLL
                     AKESFDKMRESGAMDLKYGDFAENITTEGITLYELPVGTRMRIGDTLHEVTQIGKECH
                     SGCAISKTVGKCVMPKEGIFTKVLKNGVIKSGDTIEILG"
     gene            complement(851..1303)
                     /gene="moaC"
                     /locus_tag="VFD79_01180"
                     /pseudo
     CDS             complement(851..1303)
                     /gene="moaC"
                     /locus_tag="VFD79_01180"
                     /inference="COORDINATES: protein motif:HMM:NF006870.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
     gene            complement(1307..2266)
                     /gene="moaA"
                     /locus_tag="VFD79_01185"
     CDS             complement(1307..2266)
                     /gene="moaA"
                     /locus_tag="VFD79_01185"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HZH92688.1"
                     /translation="MRDSFGRDINYLRISLTDRCNLRCEYCMPEDGIKNKIPHCDMLS
                     LEELYEITEAFVSLGIDKIRFTGGEPLVRYGAVDFIKRVNGLEGVREVTLTTNGIMLN
                     DHLDDLLDAGVKRLNISLDTLDPEKYREITRGGDIRRVLAAIENAKAKGIKPIKINTV
                     LIGGFNDDEIPDLVEMTRDGSMDVRFIELMPIGEATGFARSRFISNEEILKRIPDLVP
                     VDRDDPSSPATYYKLPGGKGKVGLINPISCKFCTNCNRVRLTATGKLKLCLHSNREID
                     LKKVLREGRDLQEAIRQAILTKEEEHHLENQEYISRNMNQIGG"
     gene            complement(2277..>3114)
                     /locus_tag="VFD79_01190"
     CDS             complement(2277..>3114)
                     /locus_tag="VFD79_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014969027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZH92689.1"
                     /translation="RLIATPLSGGAGVSMSLVKADGLGIIPRNVEGYEAGDRIRVELL
                     KPISSIRDKLLSIGSHDLVMDVVADMMKLTSSHVGSMGGILSLRRDECHLAPIHLLDM
                     ETGEYNTSYVKKYFPGKRMALIKGIQRSQGFIVQKGNPHDIKRCADLTREDLIFANRQ
                     KGAGTRVLLDYHLKMERIEPREIRGYQREFTTHMAVAAAVKNNTATTGLGVLSAAKAM
                     DLDFVPLSNEDYDFLVHGDSMEMDQVRDFVNLIKSDEFQYRVKDLGGYGFENTGEIIW
                     IE"
BASE COUNT          721 a          837 c          562 g          994 t
ORIGIN      
        1 aagcaaaagg agcttctgga ggctggattc ataggccaca gactccacag agaccaatca
       61 ttggattatg ttcaatactc ctccttatag gaaaactcca ggtgatccac ctggagtttt
      121 cttttgtagt atacatttaa ggatttagtt ttttcccgaa tatcaacgat agaacaaaca
      181 atacgaggaa cacaaagaag agtatcttgg caatttcagt tgccgctcct gcaattcctc
      241 caaagcccaa taccgcagct accaaggcca ctacaataaa tattactgac catcttaaca
      301 tttcactatc cctcctaatc tggttatgta ataggagcta tagttattat attcccttgg
      361 attgatgatc ctaaacaaaa tagcgggact aatgtcctgg tcaacctagt atctcaattg
      421 tatctcctga cttgatcact ccattcttaa gtacctttgt gaaaataccc tcctttggca
      481 tcacgcattt cccaactgtt tttgaaatgg cacagcccga atgacattcc ttgccaattt
      541 gggtcacttc atgaagggtg tcccctatcc tcattcttgt accaactgga agctcataga
      601 gtgttatgcc ctctgtggtg atgttttccg caaaatcccc atatttaagg tccatagctc
      661 cagattccct cattttgtcg aagctctcct tggccagtag acttacctgc ctgtgccagt
      721 caccgctatg ggcgtcatta acaagcccat ggtcaacctt gaattcaccc tggtctaccg
      781 gctccttcac aacacccttc ttttcagaga tattaacaga aagcaccttt ccaaagcctt
      841 cccttctgaa ggttccagac tttcctcctg tcttttccat aagctttacc tcctctattg
      901 tcatctcctt gtccatggcc ttgcacatgt catatatggt cagggctgca atgctgaccg
      961 ctgaaagagc ttccatctcc acccctgtct tgcctgtcgt ctttacctga gcccttattt
     1021 caacctcttt ttcaccaaca ttaaaggtta tgtccgctcc tgtaagaaag atgttgtgac
     1081 acatgggtat caggtcactg gttcttttgg cgcccattat tcccgcaact tgggctacgc
     1141 ttaaaacatc ccccttgtcc acgaggcctt cctttattct tcttgcagtt tcccttttca
     1201 ttgtaattct tccatatgca gttgccgtcc tttttgtatc ctccttgtca cccacctgaa
     1261 ccatgtgggc cctgccttct tcattgaagt gagtaaggtc cattgtctaa cctcctattt
     1321 gattcatgtt tctgcttata tattcctggt tttccagatg atgctcctct tcctttgtaa
     1381 gtatggcctg cctgattgcc tcctggagat ctcttccctc cctgagaacc ttcttcaggt
     1441 ctatttccct gttggaatgg agacacagct tcagttttcc tgtcgcagtt agtctcacac
     1501 gattacagtt ggtgcagaac ttgcaggaaa ttgggtttat caggccaacc ttcccctttc
     1561 cgcctggcaa tttatagtag gttgcagggg aggatggatc atccctatca accggaacaa
     1621 ggtctggaat cctcttgagt atctcctcgt tggaaataaa tcttgacctg gcaaatcccg
     1681 ttgcctcacc aattggcata agctctataa agcgtacatc catacttccg tctcttgtca
     1741 tttcaacaag gtcaggaatt tcatcatcat tgaaaccacc tattaggact gtatttatct
     1801 tgatgggttt tatacccttg gcctttgcat tttctattgc tgcaaggacc ctccgaatgt
     1861 ctcccccacg ggtgatttcc ctgtattttt caggatccag agtgtctaga cttatattca
     1921 gccttttaac tcctgcatcc aataggtcat ccaggtggtc attgagcatg attccatttg
     1981 tggtcagggt aacctccctg actccctcca gaccattgac cctcttaatg aaatcaaccg
     2041 ctccatacct tactaggggt tctccacctg taaaccttat cttgtcgatt cccaggctta
     2101 cgaaggcttc ggttatctca taaagctctt ccagactgag catatcgcag tgtggaatct
     2161 tgttttttat tccatcctcc ggcatgcaat actcgcacct aaggttgcac ctgtctgtta
     2221 aggatattct caaataattt atatctcttc caaaactgtc tctcatgtta tcaactctat
     2281 tctatccata ttatttcacc tgtattctca aaaccgtatc caccaaggtc ttttacccta
     2341 tattgaaatt catctgattt gataagattc acaaagtccc tgacctgatc catttccatg
     2401 gaatctccat ggaccaggaa atcatagtcc tcattggaca gtggaacaaa gtcaaggtcc
     2461 atggccttgg cagccgatag gaccccaagt cctgttgtag ctgtattgtt ctttacagct
     2521 gcggcaactg ccatatgggt tgtaaattcc ctctggtagc cccttatctc ccttggttca
     2581 atcctttcca tcttcaggtg ataatccagg agaaccctgg ttccagcgcc tttctgcctg
     2641 ttcgcaaata ttagatcctc ccttgtaagg tccgcacatc tctttatgtc atggggattt
     2701 cccttttgga caataaaacc ctgacttctt tgtattccct ttatcagggc cattctcttg
     2761 cctggaaagt actttttgac atagctagtg ttgtattcac cggtttccat gtccagcagg
     2821 tgtatgggtg caaggtggca ttcatccctc ctaagggaga ggatccctcc catgctaccc
     2881 acgtgtgatg aggtcagctt catcatatct gcaaccacat ccatcaccag gtcatggctt
     2941 cctatggaaa ggagcttgtc tcttatggat gatattggct ttaagagctc cacccttatc
     3001 ctgtcccctg cctcatagcc ttcaacattt cttggtatga tcccaaggcc atcggccttt
     3061 accagactca tgctgactcc tgctccacct gataatggag ttgctataag cctg
//
LOCUS       DASRQF010000047         3605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1586074, whole genome shotgun sequence.
ACCESSION   DASRQF010000047 DASRQF010000000
VERSION     DASRQF010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586074"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VFD79_01195"
     CDS             complement(<1..401)
                     /locus_tag="VFD79_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92690.1"
                     /translation="MEIKRNRVVFLLVLAMVVQVFAPAGMGIAHGEEVGSQENSMEIE
                     ELETTSGSAIEVEAETDLSSVNQFKYEANLYVGEDKLDDGSIIEIEDLTSLEYSVDFT
                     IEDGFVFEGNEYLELDLSALTELGELVLPED"
     gene            complement(590..3145)
                     /locus_tag="VFD79_01200"
     CDS             complement(590..3145)
                     /locus_tag="VFD79_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZH92691.1"
                     /translation="MEYKHEQIENILGLPFKVFIHSVDEMELHWHKHMEILFVLSGEI
                     ILNLGKEELILKEKDLILINTTDIHSTRKSEGDNLVLGMQIDTEFFNRLVPGFSRKIF
                     RLRSFEDNGHLNGKVEEIKTLLAKFTMEISKKEKGFSFRIGGLLLDLLHTIYTNFGSG
                     NMEEVDGPNRESIKRINEILRHIDDNYDKGLTLGELADRNKLNTYYLSHYIKDSLGIT
                     FQEYIDSKRLDKAISLLGETDMRITDIAYESGFPNPKSLNKLLKKKYGCTPSEYRENQ
                     TKLNTIHVRLHKPGRKKVKSYFDIETRDELKSLYDYLDHPESKHIRNLPTEKIFLEID
                     FDADSKGALKKNWNHLMTFGRAAEGLRADWRDQLEEIQKELGFRYVRFHGIFSDDMMV
                     FNRDHKGNVVYNWTYVDQLFDFLLQNNLKPFVELGFMPSEIARSQDTVFFWRGNVSPP
                     KDLDLWKNLVESFLIHCIDRYGIEEVTQWYFEIWNEPELEGLFWTGGQEEFFRFFMET
                     VACVRKVSERIRVGGPAVTHHHAIEGTFMERFLDYAKATGTKLDFASVHIYSDNFADE
                     EESKEIMSLMEDKDFARDFYNRFPSVRNIYHHMDHTEDTLDRVRDLLAKKNKGDLPLV
                     VTEWNASTSCRNPINDTSYVATHIIRNLLRSRDKADMIGYWTFTDILEEFKAGISHFH
                     GGFGLINRDGIKKPSYYAYYLLNKLGKEVLQEGEDHIVTRSRSEIQILAYNYAYFDEA
                     FKSGDTSLLSHEERYRTYAGEVDKILDFKINVSPGSYLVKRYRLDRENGSAFDKWVEM
                     GMPEDMTREEVEYLKGVSRPAIKTEIVEAESHINLSTILPPHGTELITIRKNH"
     gene            complement(3325..>3605)
                     /locus_tag="VFD79_01205"
     CDS             complement(3325..>3605)
                     /locus_tag="VFD79_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M20"
                     /protein_id="HZH92692.1"
                     /translation="IVNKKFYPYISDLSYAAAPREEKAVESLKVNMPGFGVKYNLPLD
                     EMQELNLPVVNIGPFGKDAHKFTERLEKGYSFGVLPQILLDTVNELMK"
BASE COUNT          889 a          890 c          693 g         1133 t
ORIGIN      
        1 tgatcttctg gaagtactaa ctcccctagt tcagtcaatg ctgataggtc caactccaaa
       61 tactcgtttc cttcaaatac aaatccatct tcaattgtaa agtcaaccga atactccagg
      121 ctggtcaagt cttcaatttc aatgatacta ccatcatcca gcttgtcttc ccctacatag
      181 agatttgctt catatttgaa ctgattcaca ctacttaagt ccgtttctgc ttccacctcg
      241 attgcgcttc cgctggtggt ttcaagctct tcaatctcca tggaattctc ctgagaccca
      301 acctcctcac catgggcaat ccccattcct gctggtgcaa aaacctgaac gaccattgcc
      361 aggaccaaca gaaacaccac cctgtttctt ttaatctcca tttgtataaa atcccccttt
      421 cctatttgaa acggggtcag atcatccaac cccggccata acaataaaag gttaaaattg
      481 taacctttac aatattttat tgttttaggg gatgctggta tatccaattc ttgctgtttg
      541 gaaggcactt tcttgcttca aatggaaaaa tcgcactgtt ttgtgcgatt tagtgatttt
      601 tccttatggt tataagctca gttccatggg gaggaaggat tgtggataag ttgatatggg
      661 attctgcttc aactatttca gtcttgattg caggccttga aactccttta aggtattcaa
      721 cctcctccct tgtcatgtcc tcaggcatgc ccatctcaac ccacttgtca aaggccgatc
      781 cattctcccg gtccagcctg taccttttca ccaggtagct gccaggactt acatttattt
      841 taaagtccaa aattttgtcc acttcaccgg cataggtcct gtacctttcc tcgtgagaaa
      901 gcaaggatgt gtctcctgat ttgaaggctt catcgaaata ggcgtagttg taagcaagga
      961 tctgaatttc agacctgctc cttgtgacaa tgtggtcttc accctcctgt agaacctctt
     1021 tccctagctt gttcaaaagg tagtatgcat aataagaagg cttctttatt ccatccctgt
     1081 tgatcagtcc aaaaccaccg tgaaaatgtg atatccccgc cttaaattct tcaaggatgt
     1141 ctgtaaaggt ccagtagcct atcatatccg ccttgtccct ggacctaagc aggttcctta
     1201 taatatgggt ggcaacgtag cttgtgtcat ttattggatt tctgcagctg gtggaagcat
     1261 tccactccgt caccacaaga ggaaggtctc ccttgttctt cttggctagc aggtccctga
     1321 ccctgtcaag ggtgtcctct gtgtggtcca tgtggtggta tatattcctt acacttggaa
     1381 acctgttgta gaagtccctc gcaaaatcct tgtcctccat tagggacatt atttccttgg
     1441 attcctcctc atcagcaaag ttgtccgagt aaatatggac acttgcaaag tcaagctttg
     1501 ttccagtggc cttagcatag tctagaaacc tttccataaa ggttccttca attgcatgat
     1561 gatgggtcac ggccgggcct ccgaccctta tcctttcact taccttcctg acacaggcta
     1621 cagtctccat gaagaatctg aaaaactcct cctgaccccc tgtccagaat agaccctcaa
     1681 gctccggttc gttccaaatc tcaaagtacc attgggtcac ctcttcaatt ccgtatctgt
     1741 ctatacagtg aattaggaag ctctcgacaa gatttttcca aaggtccaag tccttggggg
     1801 gtgagacgtt tcccctccag aagaacacag tatcctgtga tcgagcaatc tccgatggca
     1861 tgaatccaag ctccacaaat ggcttgaggt tgttctgaag gagaaaatca aacaactggt
     1921 caacataggt ccagttatat acgacatttc ccttgtggtc ccggttaaac accatcatat
     1981 catcagaaaa gattccatga aacctgacat acctaaaccc cagctccttt tggatttcct
     2041 ccagctggtc tctccagtca gcccttaggc cttcagcagc cctgccaaat gtcataaggt
     2101 ggttccaatt tttcttcaag gctcccttgc tgtctgcatc aaagtctatc tctaaaaaaa
     2161 tcttttctgt gggcaggttt ctgatgtgct tcgactctgg atggtccagg tagtcgtata
     2221 ggcttttcag ttcgtccctt gtctctatgt caaagtatga cttaaccttc tttcttccgg
     2281 gcttgtgcaa acgaacatgt attgtgttta acttggtttg gttttcccta tactcacttg
     2341 gggtgcagcc atattttttc ttcaggagct tgttaagtga cttggggttg ggaaatccac
     2401 tctcataggc tatgtcagtt attctcatat cagtttcacc aagtagtgag atagccttgt
     2461 ccagtctctt agagtctatg tattcctgga aggttatacc cagggaatcc ttgatgtaat
     2521 gggatagata atatgtgttg agcttgttcc tatccgcaag ctcgccaagg gtaaggccct
     2581 tgtcgtagtt gtcgtcaatg tgtcgcagga tttcatttat cctttttata ctctccctgt
     2641 ttggtccgtc aacctcctcc atatttcctg acccaaagtt tgtatagatg gtgtggagta
     2701 gatctagcaa gaggccacct atcctgaagc taaagccctt ttccttcttg cttatctcca
     2761 tggtgaattt ggccaggagg gtctttatct cctcaacctt accattcaaa tgcccattgt
     2821 cctcaaagga ccttaagcgg aatattttcc tgctgaatcc agggacaagc ctattgaaga
     2881 attcagtatc aatctgcatt cccagaacaa ggttatcccc ttcactcttc cgtgtgctgt
     2941 gaatatccgt tgtgtttatg agaataaggt ccttttcctt gagaatcaac tcctcttttc
     3001 ccagattgag tatgatttca cctgaaagta caaaaagaat ttccatgtgc ttgtgccaat
     3061 gaagctccat ctcatccacc gaatgtataa agaccttgaa gggtagccca agtatattct
     3121 ctatctgttc atgtttatac tccaaatcac ttgcacctcc atttgctata aatcaatgca
     3181 tcgatagaag tacagtacca tataatccct ctccaggcaa gattagaaac tacgtaaaca
     3241 tatggtcctg tgggtcattt tttgctgtta ggacggtaat tttttgcaat ttaaaaagcc
     3301 gaccagttgc agtcggctct ctatctattt catcaattca ttaactgtgt ccagaaggat
     3361 ctgtggaaga accccgaagg aatagccctt ctcaagtctt tctgtaaatt tatgtgcatc
     3421 cttgccaaaa ggtccgatgt tgacaacagg aaggttcaat tcctgcattt catcaagggg
     3481 taggttgtac tttaccccaa agcctggcat gttgaccttc aatgattcca cggccttctc
     3541 ctcccttggt gctgctgcgt agctaaggtc ggaaatgtat gggtagaact tcttgtttac
     3601 aatct
//
LOCUS       DASRQF010000048        14719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_218849, whole genome shotgun sequence.
ACCESSION   DASRQF010000048 DASRQF010000000
VERSION     DASRQF010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 14719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14719
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218849"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VFD79_01210"
     CDS             <1..393
                     /locus_tag="VFD79_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HZH92693.1"
                     /translation="SILEQCKKHSGCPTGEARITTAGNLPCRYVIHAVGPIYSRGDGM
                     AEELLYRAYYNSMKLADEYGLRSISFPSISTGAYGYPVREAVRVVFRALEDYSSLDTK
                     LERINLVLFSEGDLQVYKDYFNTLLSEK"
     gene            499..1143
                     /locus_tag="VFD79_01215"
     CDS             499..1143
                     /locus_tag="VFD79_01215"
                     /inference="COORDINATES: protein motif:HMM:NF013078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HZH92694.1"
                     /translation="MFKIFADTHKIIASNVYDNVLQHYDIELDKDKLLWGSIAPDILP
                     KYKLIRHYRDESINYVALEIMKIIFVSRYIDFNRITDPIAIKILSKKIGIVSHYLSDY
                     VCLPHAKRWTFNGSMVKHIRYESKLNDYAPNHTFKKNVITLDDLDIYNENPISLHSTI
                     KKYIEDVVEEYFQNKGFKNDLDFALSLNLKITYFILDVIKDYSEEIHEVFAMEI"
     misc_binding    1203..1445
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1497..2486
                     /gene="asnA"
                     /locus_tag="VFD79_01220"
     CDS             1497..2486
                     /gene="asnA"
                     /locus_tag="VFD79_01220"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005956733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HZH92695.1"
                     /translation="MMMVNGFTAREAQVGIKQIKDFFERRLADNLNLTRVSAPLFVKP
                     ETGLNDNLSGNEKAVSFQMRKYQQNLEIVQSLAKWKRMALKWYGFGDGEGLYTDMNAI
                     RPDEFVDNIHSIYVDQWDWEKVIKKEDRNLDYLKEVVVSIYEAFKETEDFINGHYPLR
                     LYKKLPEEITFVSSQELEDRWPDATPKEREHMITEEYGAVFLYKIGGALKSGKPHDLR
                     SPDYDDWELNGDILFWNPALGGPIELSSMGIRVDEDSLMKQLKLADAENRLSMEYHQK
                     LLGGELPYTVGGGIGQSRICMYFLQAKHIGQVQASVWTEDEIVRCEDEGIFLL"
     gene            2682..3416
                     /locus_tag="VFD79_01225"
     CDS             2682..3416
                     /locus_tag="VFD79_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92696.1"
                     /translation="MALSNDTFYDLYEGLKHNFLFKNDLNSIHILLNLYDIEDNIRNI
                     FPKYVSIKYLRNHISRILKNRRGNQLIAMNLGELIHEDINRLELLLYLEGYKGGFVNI
                     KEANRLEDLTVKTYSINELYYQKYLFHFDTFTEPIQDLRDEIFSNLEKDEESSNHLHN
                     LIGRYCEKVIRNKIFSLNRYLDKQLKIDYESSSANITEDETLLTMDDLTNVYNEVIKI
                     MSRNGMRLYKDAYWNGLNDRVLKRYR"
     gene            3544..4038
                     /gene="ruvC"
                     /locus_tag="VFD79_01230"
     CDS             3544..4038
                     /gene="ruvC"
                     /locus_tag="VFD79_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HZH92697.1"
                     /translation="MVILGVDPGIATVGYGVVELIGNKYRVMDYGAILTPSDQIFPLR
                     LKAVYDQLNDIIEKHRPDDLAIEELFFNKNVKTAIKVGHARGVQILAAVNHDLEVYEY
                     TPLQVKQAVVGYGRAEKRQVQEMVKILLNLDKIPKPDDVADALAVAICHGSSLKFKES
                     FKMR"
     gene            4051..4635
                     /gene="ruvA"
                     /locus_tag="VFD79_01235"
     CDS             4051..4635
                     /gene="ruvA"
                     /locus_tag="VFD79_01235"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HZH92698.1"
                     /translation="MIDYIIGKISYIGEDYFLLENQGIGYRINTSNNTLIQLIKGEDK
                     QHIFTDLIVREDSMSLYGFVSEDEMKMFKLLLTVSKIGPKVACGILSTLRPVQIQKAI
                     MLKDVTTLCKGPGVGKKTAERIVLELKDKVKGGFSEEESFDEVVEVMDNEAVEALVAL
                     GYSRYEAQKAFYGTDLNGMTVEDMIKLGLRKLSR"
     gene            4689..5702
                     /gene="ruvB"
                     /locus_tag="VFD79_01240"
     CDS             4689..5702
                     /gene="ruvB"
                     /locus_tag="VFD79_01240"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002836491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HZH92699.1"
                     /translation="MDEGRDRIVARNMNMEDNETEATLRPKWLREYIGQDKAKEKLKI
                     FIQAARGRSESLDHVLLYGPPGLGKTTLANIIANEMGVNIRVTSGPAIERPGDLASIL
                     TNLSDDDVLFIDEIHRINRTVEEVLYPAMEDFALDIIIGKGPSARSVRLDLSKFTLIG
                     ATTRAGLLTSPLRDRFGVMLNLDLYNPDNLTEIVTRSARILNIEIEPMGAYEIAKRSR
                     GTPRIANRLLKRVRDYAQVVENGIITEEIAKKGLMVLEVDKLGLDAVDRKLILTLIDN
                     FKGGPVGVDTLAAATGEERGTIEDVYEPYLLQMGFLSRTPRGRMVTEKAYRHFNKPLP
                     SEK"
     gene            5725..7104
                     /locus_tag="VFD79_01245"
     CDS             5725..7104
                     /locus_tag="VFD79_01245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HZH92700.1"
                     /translation="MKRTIIVFIVLMMIILPSTSLGRLEEDLVYFDVKVGKTYGALER
                     VRISTDGGMAIYQKNDKLDSLMELPGEIMYISLSLGRNDEIDVYDNSNTYVATLPANG
                     TVLFGPEGRGDGLMSIDGKKYRGYVSFLGNRNGLSVINHVDIESYLYGVVPKEIPSLS
                     SEEALKAQAVAARSYAYSTMDKHLNEGYNVCDTTHCQVYGGYDAEQATTNRAVDHTRG
                     LIAKYNGVVANTVFHSSSGGHTESSENVWGGRVPYLVAVEDPYSLNTINSSWEVAIDS
                     SSMNQMLLEGGIDIGEVRGIDISEKTVSGRVVELVLNGSRGTAQVSGERFRSLMGNTT
                     IKSTLFSIQGDVAPGNVDNSLIYAASGRRVQLLEGDRGKISIISGDGRVRSYSDESMT
                     VIGDGGVFTYKTPETVDISRGSVVISGRGFGHGIGMSQYGAIEMAKQGFSFEEILEHY
                     YTGIEITKY"
     gene            7121..8146
                     /gene="queA"
                     /locus_tag="VFD79_01250"
     CDS             7121..8146
                     /gene="queA"
                     /locus_tag="VFD79_01250"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004838766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HZH92701.1"
                     /translation="MKTSEFYYDLPQDLIAQHPLEDRSSSRLLVLSREKESIEHKGFV
                     DILDYLNPGDGIVLNDSRVLPARLFGNRPGKEEKLEVLLLKRTERNIWECLVRPGKKM
                     KAGGKIEFGGGILKGTVLRIGDDGTRFVEFFFDGIFEEILDQLGEMPLPPYITEKLED
                     GERYQTVYSKTPGSAAAPTAGLHFDKDLMDRISGKGIEIIYLTLHVGLGTFRPVKVDD
                     INDHIMHSEFYQISSEAAESINRIKEAGGRIISVGTTSTRTLETIADNEGRVREGSGW
                     TDIFIYPGYRFKLVDGLITNFHLPESTLMMLVSAFAGRELIMDAYREAIEKRYRFFSF
                     GDSMFIK"
     gene            8160..9281
                     /gene="tgt"
                     /locus_tag="VFD79_01255"
     CDS             8160..9281
                     /gene="tgt"
                     /locus_tag="VFD79_01255"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004833006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HZH92702.1"
                     /translation="MAIKYELIKKSTQTKARLGKITTPHGEIETPIFMPVGTRATVKT
                     MTPEEVKDLGAQIILSNTYHLYLKPGHELIEEAGGLHKFMNWDGPILTDSGGFQVFSL
                     GDLRKIKEEGVEFRSHIDGSKHFISPETAIDIQNALGSDIMMCFDECVSYPADYEYTK
                     QSMERTTRWAQRCKDHHQNWDAQGLFGIVQGGMYRDLREQSARDLLEMDFSGYAIGGL
                     SVGEPMETMCEVLDYTTPLLPEDKPRYLMGVGTPDYLFEAVIRGIDMADCVLPTRIAR
                     NGTAMTTRGKVVIRNAKYAKDFTPLDPDCDCYTCRNYSRAYIRHLFNVNEILALRLTT
                     IHNLRFLIRTMEKIREAIRKDNLLEYREEFYKNYGYSVD"
     gene            9340..9633
                     /gene="yajC"
                     /locus_tag="VFD79_01260"
     CDS             9340..9633
                     /gene="yajC"
                     /locus_tag="VFD79_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HZH92703.1"
                     /translation="MEQLQAFILPIGLLVIFYFFAIRPQKKKEKEIKEMRSGLRVGDE
                     VVTIGGIYGKIVKAKEDVLTLESGSAKTRLEITRWAVGSVLNESGDRNDETAE"
     gene            9784..9924
                     /gene="scfA"
                     /locus_tag="VFD79_01265"
     CDS             9784..9924
                     /gene="scfA"
                     /locus_tag="VFD79_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HZH92704.1"
                     /translation="MKYIKTLTKSRLKDTLSYAGCGECQTSCQSACKTSCTVGNQKCE
                     NK"
     gene            9987..11345
                     /gene="scfB"
                     /locus_tag="VFD79_01270"
     CDS             9987..11345
                     /gene="scfB"
                     /locus_tag="VFD79_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005956731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HZH92705.1"
                     /translation="MKQDLIHKFYLNEKHMVLDVNSGSVHVVDELVYNLLDHYLDSDL
                     EDIVKKMEYKYEENEVRQAYEEVGFLEENGMLYSDSEDISNLTYNQDNIVKAMCLHVA
                     HDCNLKCEYCFASQGNFKGERSMMDLETGKKALLYLAQNSGNRRNLEVDFFGGEPLMN
                     FGTVKDLVDYGRSIEQEYNKHFRFTITTNGVLLDDDKIQYINENMDNVVLSLDGRKTV
                     NDAMRLTVGGEGSYDVILPKIKKMVQARGEKDYYVRGTFTSKNLDFGKDAINFHREGF
                     KKISIEPVVAPEEMDYAIREDHLGRILQEYEDFSKEYIRLTQEDKDFMFFHFMIDLDQ
                     GPCVVKRAVGCGAGSEYLAVTPEGELYPCHQFVGEKEFLLGTLETGIQNRELRDKFKL
                     ANVYNKEECRNCWARFYCSGGCHANAHYAHNDIMKPYEIGCEMEKKRIECALSVLANG
                     ES"
     gene            11356..12621
                     /gene="secD"
                     /locus_tag="VFD79_01275"
     CDS             11356..12621
                     /gene="secD"
                     /locus_tag="VFD79_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HZH92706.1"
                     /translation="MKSRSMAILAVIIAIVIGLAFFAVNGATFGDTQYKNVKESIELG
                     LDLAGGVYVVLEANTDATGEELRRMMEETKAIIGHRVDGLGVSEPNIAIEDEDRIRIE
                     LAGLSDPQEAIELIGKTAMLEFKTPVGETVITGKHVKSAGVVMNPSQFGGEEPVVSLE
                     FNDEGIDLFRAATAALAAEENDMDKILYIVLDGVVISSPIVDEAINDGRAIITGGFTI
                     ESAGNLATLISAGALPVEMIELQTSVIGPTLGLEAYERSILAAGVALLLIFAFMIIVY
                     RVPGLVASLALIVYVLLVVGSMMGVGVKLTLPGIAGLVLSIGMAVDANVLIYERIREE
                     IQVGKSVRVSIDSGFKRALTSILDANITTLIAGIVLYSFGTGPIKGFGVTLIIGIGAS
                     MITAVFFTKYVLRLMAHITGGKNIKLYGA"
     gene            12632..13489
                     /gene="secF"
                     /locus_tag="VFD79_01280"
     CDS             12632..13489
                     /gene="secF"
                     /locus_tag="VFD79_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HZH92707.1"
                     /translation="MNIIKNRKYSYMVSLAIILAGLIMFFINGLNYGIDFTGGTMIQL
                     NIGSFVEVEDAREILDAFDENASIIHAGSNREEIIIRSSLDLENNQISNIVDQFVLKF
                     QMDPENYQAQKFGAFMGKEIRDRAILSIGITSILMLGYITFRFQFKFGVAAIVALIHD
                     ILIALSIYSLLRLPVNSSFIAAMLTIVGYSINDTIVIFDRIREELSFNQKKGLEETIE
                     DSVKLSLRRTINTTITTVIAVGSLYVLGVEDVKVLALPLLLGMLSGTYSSLFIATPVW
                     FSLKKGKTV"
     gene            13515..13877
                     /locus_tag="VFD79_01285"
     CDS             13515..13877
                     /locus_tag="VFD79_01285"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HZH92708.1"
                     /translation="MQKSFILILVLAIIIGIFALSNSDVVEIDFVFARIMLSQAIVIF
                     ISVLLGAAVATLFGVLREMKLKKQIKENKNGMEALKSENKSLLMRVEEKEEQLKLLYG
                     RKEESPDQVDMENDFSGN"
     gene            13942..>14719
                     /locus_tag="VFD79_01290"
     CDS             13942..>14719
                     /locus_tag="VFD79_01290"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HZH92709.1"
                     /translation="MEKWFIRNNPGDVNKISMENNISKPLAKILTNRGLTDRVDIESF
                     LNPDIKKLHKPLLMEDMDAAGRLILDKIRKGKKILIVGDFDVDGIMSTYILYSTLKEL
                     GADVDYTIPDRIQDGYGINTHIVLNAKASGVDTIITCDNGIAAIEPIETGKNMDMTII
                     VTDHHDIPQSGMPKADCILNPKKPGCTYPEKNLCGAGVAFKLASHLAKSAKGKVNLKP
                     LLEMVAIATICDVVDLVGENRIFVKEGLELLNKTSNLGLRA"
BASE COUNT         4779 a         2489 c         3582 g         3869 t
ORIGIN      
        1 tcaatactgg aacagtgcaa gaagcattct ggctgtccaa ctggcgaggc tagaatcaca
       61 acagcaggaa acctgccctg cagatacgtt atacatgctg ttggcccaat ctactccaga
      121 ggggacggaa tggcggaaga gcttctgtat agagcctatt acaactccat gaaactggca
      181 gatgagtatg gtttaagatc aatatctttt ccatccattt caactggagc ctacggatac
      241 ccggtaagag aggctgttag ggtagttttt agggcattgg aagactattc tagtctggat
      301 acaaaattgg agaggattaa tctagtatta tttagtgaag gtgacctcca ggtctacaaa
      361 gattatttca acactttact ctcagaaaaa taaatttcct taaaaatgaa atattaagat
      421 aatgcaacat ttgattgatt gacataagat ttcatatgtt atatcatcaa aggagttgga
      481 aaaacaggag aaaagggagt gtttaaaatt tttgcagata ctcacaagat aattgcctcc
      541 aatgtatatg acaatgttct acaacattat gatatagagc tggataagga caagttactc
      601 tggggctcca tagcaccgga tattctacct aagtacaagc tcataagaca ctacagggat
      661 gagagcatca actacgttgc attggaaatc atgaaaataa tttttgtaag ccgatatatt
      721 gatttcaata ggataacaga tccaatagcc atcaagattc tcagtaagaa aataggaata
      781 gtttctcatt atctttctga ttatgtttgc ctaccacatg caaaaagatg gaccttcaac
      841 ggatcaatgg ttaagcatat cagatatgag tcaaagctaa acgattatgc cccaaaccat
      901 acctttaaga aaaatgtcat taccttggat gatcttgaca tttacaatga aaatccaata
      961 agccttcatt ctactattaa gaagtacatc gaggacgttg tggaggagta tttccaaaac
     1021 aagggcttta agaatgatct ggattttgcc ttgtctttga atcttaagat aacatatttc
     1081 attcttgatg taatcaagga ttactcagag gagattcacg aagtatttgc aatggagata
     1141 tagtatttat aaataactat tgacaagcac aacctaagtc tatatactaa catataagtt
     1201 aatacactac attgaaggga agagtagtgc aggaaataag tttaagcgat tcagggacgg
     1261 tgggagcctg atacgaaagc ttgcatgaag aacagcccgg agtatctgat cgaaagacga
     1321 tgtctagtag actcagacgg tatagcccgt tacaagctat gacgtatcga ttaatttctg
     1381 tacggaatga gagagcatta ttgctaattt aggtggtacc gcgaaagtaa cctttcgtcc
     1441 tttatatgag gacggaaggt ttttttaata caaaaaataa caagggagtg ataagaatga
     1501 tgatggtaaa tggttttaca gctagagaag cacaagttgg aatcaagcag atcaaagatt
     1561 ttttcgagag gagactggcg gataatctta atctaaccag agtatctgca ccactttttg
     1621 taaagccaga aactggtcta aatgacaatc tctccgggaa cgagaaggca gtttccttcc
     1681 aaatgagaaa atatcagcag aatctggaaa ttgtacaatc tctggccaaa tggaaaagaa
     1741 tggcacttaa atggtacggc tttggagatg gagaaggact ttatacggat atgaacgcca
     1801 taagacctga tgaatttgta gataacatac attcaattta tgtagatcag tgggattggg
     1861 aaaaagtgat aaagaaagag gacagaaatc tggattacct gaaagaggtt gtagtatcaa
     1921 tttatgaagc ctttaaggag acggaagatt tcataaatgg gcattatccc ctgagacttt
     1981 acaagaagct accagaggaa ataacctttg taagcagtca agaacttgaa gatagatggc
     2041 ccgatgccac tccaaaggaa agagagcaca tgattaccga ggagtatgga gcagttttcc
     2101 tatacaagat aggaggagcc cttaagtcgg ggaaacctca tgatctaaga tcaccagatt
     2161 atgacgattg ggagttaaac ggagatatac ttttctggaa ccctgcactt ggtggaccca
     2221 ttgagctttc gagtatggga ataagagttg atgaagattc ccttatgaaa caactgaagc
     2281 ttgctgatgc tgaaaacaga ttgtccatgg agtaccatca gaagcttttg gggggagagc
     2341 ttccctatac agtaggtgga ggaattggac aatccagaat ctgcatgtac tttctccagg
     2401 caaaacacat cggtcaggta caggcatctg tatggacaga agacgaaata gtaaggtgtg
     2461 aggatgaagg gatcttcctt ctatagctaa acatcattta ttaaaccaga taacttattt
     2521 gcccggtctt aacagggagg aaagacgaca tcctcctttg ttaagatttt gtaacaaatc
     2581 aatccaattt tattaaacat ttgaaggctg ttttatagta taatagaaaa taccgccacc
     2641 caaatttcat ggtggaatat tcttaaggag ggatgctata gatggcttta tctaatgaca
     2701 cattttatga tctatacgaa ggattaaagc acaacttttt atttaagaat gatcttaata
     2761 gcatacatat ccttttgaat ctgtacgata ttgaggacaa catcagaaat atttttccca
     2821 aatatgtttc catcaagtac ttgagaaacc acatttccag aatcctcaag aacagacgtg
     2881 gcaaccagct gatagcaatg aacctgggag aacttattca cgaggacata aacagactgg
     2941 agctgctact ttatctggag ggctacaaag gtgggtttgt taatatcaag gaagcaaaca
     3001 ggcttgagga tctgacagta aaaacctatt caataaatga actgtattac caaaagtatc
     3061 tgtttcactt cgatacattc actgagccta ttcaggatct tcgtgatgag atattctcaa
     3121 atcttgaaaa ggatgaggag tccagcaatc atctacacaa cctaattggc agatattgtg
     3181 agaaggtaat cagaaataag atattcagcc taaacagata cctggacaag cagcttaaaa
     3241 tagactatga atcctcctct gcaaacatca ccgaggatga aacattactt acaatggatg
     3301 atcttacaaa tgtatacaat gaagttataa aaatcatgtc cagaaatgga atgagactgt
     3361 ataaggacgc ctactggaac ggcttgaatg acagggtcct taaaagatat cgatagagca
     3421 atcattaata atgcataata ccaccctctg acttttatgg tcagggggtt ttaaattcaa
     3481 gtttagattt gcatggatta tatggtacaa tagataaagg attgatgctg aaagaggtga
     3541 cgtatggtaa ttttaggagt ggacccggga atagccacag tgggttatgg cgtggtggaa
     3601 ttgattggaa acaagtacag ggttatggat tatggagcta ttctgactcc ttctgatcaa
     3661 atatttcccc taaggttgaa agctgtatat gaccagctca atgatataat agaaaagcac
     3721 agaccagatg atctggctat tgaggagctc tttttcaata aaaatgttaa gactgccata
     3781 aaggttggtc atgcaagagg tgtccaaatc cttgctgctg tcaatcacga cctggaggta
     3841 tacgagtaca cacctcttca ggttaaacaa gccgttgtgg gatatggaag ggctgagaag
     3901 aggcaggtcc aggaaatggt aaagattctt ctaaatctgg ataaaatacc taaaccggat
     3961 gatgttgcag atgccttagc agttgcaatt tgtcacggaa gttccttgaa attcaaggaa
     4021 tcattcaaaa tgagataagt aggtggaata atgattgatt atataattgg gaaaatcagt
     4081 tacataggtg aagattattt cctccttgaa aaccagggga taggctatcg aataaacaca
     4141 tccaacaata cgttgatcca gctgattaag ggtgaggata aacagcacat attcactgac
     4201 cttattgtta gggaggattc aatgagccta tatggttttg tcagtgagga tgagatgaag
     4261 atgttcaagc tcttgctgac tgtatcaaaa atcggaccca aggttgcctg tggaatacta
     4321 tccaccctaa gacctgttca aattcagaag gcaatcatgc ttaaggatgt tacaaccctt
     4381 tgcaagggac ctggtgtggg gaaaaagact gctgaaagga ttgttcttga gcttaaggac
     4441 aaggtaaagg gaggcttcag tgaggaggaa tcctttgatg aagttgttga agtcatggat
     4501 aatgaagcag tcgaggctct tgtcgccttg ggatatagca gatatgaggc acagaaggca
     4561 ttttatggta ctgacttaaa tggaatgacg gttgaggata tgataaagct gggattgaga
     4621 aagctgtcca gatagcgatt gggacctgca agctcttata aatttaaatt acaatgggag
     4681 atatacttat ggatgaagga agagatagaa tcgttgccag gaatatgaat atggaggaca
     4741 atgagaccga ggccacactt agacccaagt ggcttagaga atatatcggt caggacaagg
     4801 ccaaggaaaa actgaagatt tttattcagg cagcaagagg tagaagcgag tcactggatc
     4861 atgttcttct ttatggacct ccaggacttg gtaagacaac actggcaaac ataattgcaa
     4921 atgagatggg agtcaacatt agggtaacat caggaccggc tatcgaaaga ccgggagact
     4981 tggcaagcat actcaccaac ctctcagatg atgatgttct ttttattgat gaaatccatc
     5041 ggatcaacag aaccgttgag gaggtccttt atcctgccat ggaggatttt gccctggata
     5101 tcataatagg gaagggaccc tctgcaagat ctgtcagact ggacttgtca aagtttaccc
     5161 tcataggagc cactacaagg gcaggcctat taacatcacc attaagggat aggtttggtg
     5221 tcatgctgaa tctggatctt tacaatccag ataacctaac agagattgta acaagatcgg
     5281 ccaggatact aaatattgaa attgaaccca tgggtgccta tgaaattgcc aagaggtcaa
     5341 gaggtacccc aaggattgcg aacaggctgc taaaaagagt cagggactat gctcaggtgg
     5401 ttgaaaatgg tattattaca gaggaaatag ccaagaaggg cctaatggtt cttgaggtgg
     5461 ataaactcgg gcttgatgca gttgatagaa agcttatcct gacactgatt gataatttca
     5521 agggaggccc tgttggggtg gatacactgg cagctgccac aggtgaagaa agaggaacaa
     5581 ttgaagatgt atacgaaccc taccttttac aaatgggatt cctaagcagg acaccaaggg
     5641 gaagaatggt aactgaaaag gcctacaggc atttcaacaa acctcttcct tcagaaaaat
     5701 aataacataa ggctggtgtg agacatgaaa agaaccataa tcgtttttat tgttttaatg
     5761 atgataatcc taccatccac ttcacttgga agacttgagg aggatctggt ctattttgac
     5821 gttaaggtag gcaagaccta cggagctctt gaaagggtga ggatttcaac ggatggtggc
     5881 atggcaatat atcagaagaa tgacaagcta gatagtttga tggagcttcc cggcgagata
     5941 atgtacataa gcttgagctt gggcagaaat gatgaaattg atgtgtatga taattcaaac
     6001 acctatgttg cgaccctgcc ggcaaatggt acagtcctat ttggaccaga gggcagaggg
     6061 gatggcctga tgagcattga tggtaaaaaa tacaggggct atgtttcctt tttggggaat
     6121 agaaacgggc tttctgtaat aaaccatgtt gatattgaaa gctatcttta tggtgttgtc
     6181 ccaaaggaga taccatccct aagcagtgag gaggccttga aggcccaggc tgttgcagcc
     6241 aggagctatg cctactcgac catggacaag caccttaacg agggttacaa cgtctgtgac
     6301 accacccatt gtcaggtata cggtggctac gatgctgaac aggcaaccac aaacagggca
     6361 gttgaccaca caaggggatt gattgctaag tataatggtg ttgttgcaaa tacagtattc
     6421 cattcatcct ctgggggaca tactgaaagc agtgaaaatg tatggggagg aagggttccc
     6481 tatctggtgg ctgttgagga cccttattcc ttgaatacaa taaatagcag ttgggaagtg
     6541 gcgattgact cttccagcat gaatcagatg cttttggaag gcggaattga cataggagag
     6601 gtaagaggca tagatatttc ggagaaaact gtttccggta gggtcgtgga gctagtcctg
     6661 aacgggtcaa ggggaactgc ccaggtttca ggagaaaggt ttagaagtct gatgggaaat
     6721 acaacaataa agagtaccct tttttccatt caaggcgatg tagcccctgg taatgtggat
     6781 aattcactta tctatgcggc ctctgggaga agggttcagc ttttggaagg agacaggggg
     6841 aaaataagca taatatctgg tgacggcagg gttaggtctt acagcgatga atccatgacg
     6901 gtaattggtg atgggggtgt attcacctat aaaacaccgg aaactgttga catatcaaga
     6961 ggaagtgttg taatatctgg aaggggtttc ggacacggca taggaatgag tcaatatgga
     7021 gcaattgaaa tggcaaagca aggcttcagt tttgaagaaa ttctagagca ctattatacc
     7081 ggtattgaaa tcaccaaata ctaaattaag gaagttgaaa atgaaaacaa gtgaatttta
     7141 ttatgacctt ccacaggacc tgatagctca gcatccattg gaggataggt caagctcaag
     7201 acttctggtc ttgagtaggg aaaaggagag catcgagcat aaaggctttg tagatatttt
     7261 agattatctt aatcctggag atgggatagt gttgaacgat tccagagtat tgcctgcaag
     7321 gctatttggc aatagaccag gcaaggaaga aaaactggaa gtgcttcttt taaagaggac
     7381 tgaaaggaac atctgggaat gccttgtaag accgggcaaa aagatgaagg ctggtggaaa
     7441 gatagagttt ggtggtggaa tcctcaaggg aacggtcctt aggattggtg atgatggaac
     7501 cagattcgta gaatttttct ttgatggaat ttttgaggaa atcctcgacc aacttggaga
     7561 aatgccattg ccaccctata taacagaaaa acttgaggat ggggaaagat accagacggt
     7621 ttattctaaa actccaggat cagcagctgc tccaacagca ggcttacact ttgacaagga
     7681 ccttatggac aggataagcg gcaaggggat tgaaatcata tacctcaccc tgcatgtggg
     7741 gcttggaacc tttaggccgg ttaaggtaga tgatataaat gatcacatca tgcattcgga
     7801 attttatcag ataagtagtg aggctgcaga aagcatcaat agaataaagg aagctggagg
     7861 aaggataata tccgttggaa ccacttcgac caggaccctt gaaaccattg ccgacaatga
     7921 aggaagggta agggaagggt ctggttggac agatattttc atttatccag gctacagatt
     7981 caagctggtg gatggactta taactaactt tcatctgcct gaatccacac taatgatgct
     8041 tgttagtgca tttgcaggaa gggaattgat aatggatgcc tatagggaag cgattgagaa
     8101 gagatatagg ttctttagct ttggggattc aatgttcata aaataatcgg gaggtaaaaa
     8161 tggcaattaa gtacgaattg ataaagaagt ccacacagac caaggctaga cttgggaaaa
     8221 tcacaactcc tcatggtgaa atagagacac caatatttat gccggttggg acaagggcaa
     8281 cagttaagac catgactcct gaagaggtca aggatctggg tgcccagata atactgtcca
     8341 acacctacca tctttatcta aagccaggtc acgagcttat agaggaagcc ggaggacttc
     8401 ataaatttat gaactgggat gggccaattc taactgatag cggagggttc caggttttca
     8461 gcctgggtga ccttagaaaa atcaaggagg agggagttga atttcgctcc catattgacg
     8521 gttcaaaaca tttcattagc cctgaaacag ctattgatat acagaatgca ctaggttcgg
     8581 atattatgat gtgttttgac gaatgtgtct cctatccagc tgattatgaa tatacaaaac
     8641 aatccatgga aaggaccact agatgggctc aaagatgcaa ggaccatcat caaaactggg
     8701 atgcccaggg tctttttgga atagtccagg gtggaatgta cagagacctt agggaacaaa
     8761 gtgcaaggga ccttttggaa atggatttta gcggctatgc tataggagga ttgagtgtgg
     8821 gtgaaccaat ggagacaatg tgcgaggttc tggactatac gacacccctc ttgcctgagg
     8881 ataagccaag gtacctgatg ggtgtaggaa ctcctgacta cctgtttgaa gctgtaatta
     8941 ggggcataga catggctgac tgcgttctgc ccaccaggat agccagaaac ggaactgcca
     9001 tgaccacaag gggcaaggtg gtcattagaa atgccaagta cgccaaggat ttcacgcctt
     9061 tggaccctga ttgcgattgc tatacctgca ggaactattc gcgggcatat attagacatc
     9121 ttttcaatgt taatgaaata ctggctttga gactgactac aatccataat ctaagattcc
     9181 tgataagaac catggaaaag attagagagg ccataagaaa ggacaatcta ttggagtata
     9241 gggaggaatt ttacaaaaat tatggctatt cagtagatta aggggtgatt ttttcgccat
     9301 ttcatgtata atatatataa gaaaaggagt gattatttaa tggagcaact gcaagcgttt
     9361 attcttccaa ttggactttt ggttattttc tatttctttg ccatcagacc acagaagaaa
     9421 aaagagaaag aaatcaagga aatgagaagc ggacttagag taggtgacga ggttgtaaca
     9481 atcggtggaa tttatggaaa aatcgtaaag gccaaagaag acgtcttgac cctggagtca
     9541 ggttctgcaa aaacaagatt agagatcaca agatgggcgg ttggttctgt acttaatgaa
     9601 agcggtgaca gaaacgacga aacagcagag taagctgaat ataaagaata aaacttcctt
     9661 cgccggaagt ttttcttttt tccaccaggt gagtttgctc aaacaaaaac tgctttataa
     9721 tacacacccc ttgtggtata attgttccat aaaaacctgt tgaccaggga taggaggaag
     9781 aatatgaagt acataaagac attgacaaag agcagactga aggatactct ttcctatgcc
     9841 ggttgtggcg agtgtcagac atcctgccaa tcagcatgta agacatcctg tacagttggc
     9901 aatcaaaagt gcgaaaataa gtagcataat gaagagtagt gggttctcca ctgcttttta
     9961 tttgaaaaaa atgaggagga aataagttga aacaagactt gatacataag ttctatttaa
    10021 atgaaaagca catggtactt gatgttaata gcggttcagt ccatgtggtg gatgaactgg
    10081 tttacaatct gctggaccat tacctggata gtgatctgga agatatcgtt aagaaaatgg
    10141 aatataaata tgaagaaaat gaagtacgcc aagcctatga ggaagttgga ttccttgaag
    10201 aaaatgggat gctgtattca gattccgagg atatctcaaa cctaacctac aatcaggaca
    10261 acatagttaa ggccatgtgt ctccacgtag cccatgattg caatctgaag tgtgagtatt
    10321 gcttcgcatc ccagggtaat ttcaagggcg aaaggtccat gatggatctt gagacgggaa
    10381 agaaggcctt gctataccta gcacaaaact caggcaacag aagaaatctg gaggttgact
    10441 tctttggtgg tgaaccactg atgaattttg ggaccgtcaa ggatcttgtt gactacggaa
    10501 gaagcattga gcaggagtac aacaagcatt tcagatttac aataacaaca aacggtgtcc
    10561 ttttggatga tgataagatt cagtatatca atgaaaatat ggataatgta gttctgagcc
    10621 tggatggaag aaagacagta aacgatgcca tgaggcttac tgttggcgga gagggaagct
    10681 acgatgtgat cttacccaaa atcaagaaaa tggttcaggc aagaggagaa aaggactatt
    10741 atgtcagggg gaccttcaca agcaagaacc tggattttgg aaaggatgca ataaacttcc
    10801 acagggaggg gttcaagaag atatctattg agccagttgt tgcaccagag gaaatggact
    10861 atgccatcag agaggaccat ctgggtagaa tcctacagga atacgaggat ttttccaaag
    10921 agtacatcag attgacccag gaggacaagg atttcatgtt tttccacttt atgattgact
    10981 tggaccaggg tccgtgtgtg gtcaaaaggg cagtgggttg tggtgctgga agtgaatacc
    11041 tagcggtaac acctgaaggg gaattatacc cttgccacca gtttgtcggt gaaaaggagt
    11101 ttcttctggg cacactggaa acgggcattc aaaaccgtga acttagggac aagtttaagc
    11161 tggccaatgt atacaacaaa gaagagtgcc gcaactgttg ggctaggttc tattgtagtg
    11221 gcggttgcca tgcgaatgct cactatgccc acaatgatat tatgaaaccc tatgaaattg
    11281 ggtgtgagat ggagaaaaag aggatagagt gtgctctttc tgttttggca aacggagagt
    11341 cttaggaggt aggggatgaa atcaagaagc atggccatac tggctgtaat aattgcaatt
    11401 gtaataggtt tagcattctt tgccgtaaat ggggccacat ttggggatac ccagtacaaa
    11461 aatgtcaagg aatcgataga attggggctt gatttggcag gcggtgtgta tgtggtgctt
    11521 gaagcaaaca cagatgcaac aggggaagag ctgcgcagga tgatggagga gaccaaggca
    11581 atcatcggac accgtgtaga tggactgggg gtatctgagc cgaatattgc cattgaggac
    11641 gaagatagaa taagaattga gttggcaggg ctgagtgatc cccaagaggc cattgaattg
    11701 ataggaaaga cagcaatgct tgaatttaaa acccctgtgg gggaaacagt catcacagga
    11761 aagcacgtta agagtgcagg tgttgtgatg aacccatctc aatttggagg agaggagcca
    11821 gtagttagct tagagttcaa tgatgaggga atagaccttt tcagagcagc aactgcagct
    11881 cttgctgctg aagaaaatga catggacaag attctctaca ttgtccttga tggagtggtc
    11941 atatcaagtc caatagtgga tgaagctatc aatgacggta gggcaatcat cacaggtggg
    12001 tttacaatag aaagtgcagg aaaccttgcc acattaataa gtgcaggagc cttgccggtt
    12061 gaaatgattg agcttcagac atctgtaatt ggaccaacac ttggcttgga agcctatgaa
    12121 aggagcatat tggcagctgg tgttgcattg ttgctgatat ttgcctttat gataatagtt
    12181 tacagggtac ctggtctggt tgcatcactt gccttgattg tctatgtgct tcttgtggta
    12241 gggtcaatga tgggagtagg tgtaaaactc accctccccg gaattgctgg tttggttctt
    12301 tcaataggta tggccgttga tgccaatgta ttgatttacg aaagaattcg ggaagagatt
    12361 caagttggaa aaagcgtaag ggtatcgatc gactcgggat ttaagagagc attaacatca
    12421 attctggatg ccaacattac cacactcatt gcaggcatag tcttgtatag ctttggaaca
    12481 ggaccgatca agggctttgg agtcaccttg ataattggta ttggtgcgtc tatgattaca
    12541 gcagtgtttt ttacaaagta tgtattgaga ttgatggcac acataaccgg cgggaaaaat
    12601 ataaagcttt atggagcata ggagggaaag aatgaatatt ataaagaata gaaaatactc
    12661 atatatggtt tccctggcca taattctagc cggcttgatc atgtttttca tcaatggatt
    12721 aaactatggt atagatttta caggtggaac aatgatacaa ctaaatatcg gatcctttgt
    12781 ggaggttgag gatgcaaggg aaatattgga tgcatttgac gaaaatgcaa gcatcattca
    12841 cgcaggatca aacagggaag agattatcat aagaagttcc cttgacttgg agaacaatca
    12901 aatttctaat attgtagacc agtttgttct caagtttcag atggaccctg aaaattatca
    12961 agctcaaaag tttggggcat tcatggggaa ggaaattcgg gatagggcaa tactatccat
    13021 cggaataact tccatactaa tgcttgggta tatcaccttc agatttcagt tcaagtttgg
    13081 agtcgctgca attgtggccc tgatccatga tatcctgata gcactgtcaa tatattcatt
    13141 actcagactt ccagtaaaca gctccttcat agctgccatg ctgacgatag ttggatactc
    13201 aataaacgat accattgtaa tatttgatcg aatcagggaa gagttgagtt tcaatcaaaa
    13261 gaagggtttg gaagagacaa ttgaggacag cgtaaaactt tcattgagaa gaaccataaa
    13321 cacaacaata acaacggtga ttgccgttgg atccctatat gtattgggag tggaggatgt
    13381 aaaggtactt gctttgccgc tactgctggg aatgctctct ggaacctatt cctccctatt
    13441 tattgcaaca cctgtatggt ttagcctaaa aaagggtaaa acagtataga gagatcaaac
    13501 aagggggtat tgagatgcag aaaagcttta tacttatact ggttctggca attatcattg
    13561 gcattttcgc tttaagtaac agtgatgtgg tggagattga ttttgtattc gcaaggataa
    13621 tgctttccca agctatagtg atttttattt cggttctgct tggagctgca gttgctacac
    13681 tatttggtgt attaagggaa atgaagctca agaaacagat aaaggaaaat aagaatggaa
    13741 tggaagctct caaatcagag aacaagtctc tacttatgag agttgaggaa aaggaagaac
    13801 aattgaaact attgtatgga agaaaagagg agtctcctga tcaagttgac atggagaatg
    13861 atttttctgg aaattagacg gacaaagtgg tagaatttag ataatattat ctaaattcta
    13921 tttttttatt ggagtgattt tttggaaaag tggtttatta gaaacaatcc tggtgatgta
    13981 aacaaaatat caatggaaaa caatatttcc aagcctctgg ctaagatttt aacaaataga
    14041 ggattaactg atagagttga catagagtca tttctgaatc cagatataaa aaagctacac
    14101 aagcccttgc taatggagga catggatgca gctggcaggc tcatactcga taaaatcaga
    14161 aaagggaaga agattctcat tgttggagat tttgacgttg atggcataat gtcaacatat
    14221 atactataca gtaccttgaa ggaacttgga gcagatgtag actatacaat acccgataga
    14281 attcaggatg gctatggcat aaatacccat attgttctga atgccaaggc atcaggagtt
    14341 gatacaatca tcacctgtga caatggaatt gcggccattg aacccattga aacgggaaag
    14401 aatatggata tgaccattat tgttactgac caccacgata ttccccaaag cgggatgccc
    14461 aaggcagact gcatacttaa tccaaagaag ccaggctgca cttatcccga gaagaacctg
    14521 tgcggtgcgg gagtggcatt taagctggcg agtcatttgg caaaatcagc caaggggaag
    14581 gttaacctga aaccactcct ggaaatggtg gcaatagcaa ccatatgtga tgttgttgac
    14641 cttgtgggag aaaacagaat ttttgtgaag gaaggcctgg aattgctgaa taagacctcc
    14701 aatttgggac taagagccc
//
LOCUS       DASRQF010000049         2565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1212554, whole genome shotgun sequence.
ACCESSION   DASRQF010000049 DASRQF010000000
VERSION     DASRQF010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212554"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="VFD79_01295"
     CDS             <1..1067
                     /locus_tag="VFD79_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HZH92710.1"
                     /translation="ETVAFGDPGFVKDFKEVLTDLKDLWDVDGEVFVVAGSGTMAMEM
                     ALANTLKRGDNLLIVSHGFFGDRFIEIAEKKGLNVDVISSEWGKTVPVADIEKKLGEK
                     TYQAITVTHVDTSTAAVAPIAEIGEMMKKFPETIYIVDGVAATAGEEEYLGKMNIDIL
                     FTGSQKAFGVAPGLAILFANQKALDRRKSLGNIQEYYIDFEKWIPIMNDTSKYYATPA
                     VNMIWALQESIRLIKEEGLEERYERHRKVAKAMRAALEAMGFSILADPENRAVTLSCV
                     LYPEGVEDVEFRTLLAQEGAQVAGGLGAYAGKMFRIGHMGNIDMLDLTGAITAIERTL
                     YKMGVDVLGKGVSALTKELQ"
     gene            complement(1123..1581)
                     /locus_tag="VFD79_01300"
     CDS             complement(1123..1581)
                     /locus_tag="VFD79_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZH92711.1"
                     /translation="MKRILVPVDGSPASMKAALLAIDVAKNYGSEITFFSVVRRPELI
                     GTAVGGGIGYVGDMRKVVEGLTERQRRMLDEFVDGLNCRDIECTKDIIVGEPYEEIIS
                     HAKYIEADLIVMGRRGFSKVQRFFLGSVSQRVVSDSPCPVLVVNEDVEGE"
     gene            1850..>2565
                     /locus_tag="VFD79_01305"
     CDS             1850..>2565
                     /locus_tag="VFD79_01305"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HZH92712.1"
                     /translation="MPKSRGLSFIIIALVSVAVLFIGGSMMNSGGGAFAEIPDGTYEG
                     TSDAGMYPGLKVAVTFAGGSITNVEVVEHDETPGISDPAIEGVPAAIVAANSTEVDVV
                     SGATLTSNAIIEAVNNAIAVASGNAPAVVYEDGVYEGTSDKGMHPGLKVSVTVEGGKI
                     TNVEVVEHDETPGIADPAIEGVPAAIVEAGSPNVDVVSGATLTSEAIMDAVQNALSGG
                     SGEEEPAEEEPAVEEPDIST"
BASE COUNT          778 a          558 c          617 g          612 t
ORIGIN      
        1 gagagacagt ggcattcggt gatccaggat ttgtaaagga tttcaaggaa gtacttacag
       61 accttaagga cctttgggac gttgatggag aggtatttgt agttgcggga tctggaacaa
      121 tggcaatgga gatggccctt gcaaacacac ttaagagggg agacaatctc ctgatcgtat
      181 cccacggctt ctttggggac aggttcattg agattgccga gaaaaaggga cttaatgttg
      241 atgttatttc ttcagagtgg gggaaaacgg tacctgtagc tgatatcgag aaaaagcttg
      301 gtgaaaagac ctaccaggca attacagtga cccatgttga cacatcaact gcagcggttg
      361 caccaatcgc tgaaataggt gaaatgatga agaagttccc agagacaatc tacattgtgg
      421 acggagttgc tgcaacagca ggggaagagg aatacctggg caagatgaac atagacatcc
      481 tgttcacggg atcacagaag gcatttggag tcgctccagg acttgcaatc ctctttgcca
      541 accagaaagc ccttgacagg agaaaatcac ttggaaacat ccaggaatac tatatagact
      601 tcgagaagtg gattcctatc atgaacgaca cttcaaagta ctacgctaca ccagcggtta
      661 acatgatatg ggcccttcag gaatcaatca gacttatcaa ggaagaggga cttgaggaaa
      721 gatacgaaag acacagaaag gttgccaagg caatgagggc tgcccttgag gcaatgggct
      781 tctcaatcct ggcagacccg gaaaacaggg ccgtaaccct atcatgcgta ctctatcctg
      841 aaggtgtgga ggatgtggaa ttcagaaccc tacttgccca ggaaggcgct caggttgcag
      901 gcggacttgg agcatatgca ggcaagatgt tcaggatcgg tcacatgggc aacatcgaca
      961 tgcttgacct aactggggca atcactgcca tagagagaac cctttataaa atgggagtgg
     1021 atgtccttgg aaaaggtgta tccgcattga caaaagagct tcaataaaca aacaataaat
     1081 ccctgcgggt accaagaccc acagggattt ataagtttcc cgtcattctc cttcaacatc
     1141 ctcgttgacc accagtacag gacatggtga atctgataca accctttggg aaacagatcc
     1201 caggaaaaac ctctgcacct ttgaaaaacc cctcctgccc attacaataa ggtctgcttc
     1261 aatgtatttg gcatggctga ttatctcctc gtagggttcg ccaacaatta tatccttggt
     1321 gcactcaata tccctacagt taagtccatc aacgaactcg tcaagcatcc ttctctgtct
     1381 ttccgtcaaa ccctcgacga ccttcctcat atcacccaca taaccaattc caccgcccac
     1441 ggcagttcca atcaactcag gtcttctaac tactgaaaaa aaagtgatct cactgccata
     1501 gttcttggcc acatcaatgg ccagtagggc agccttcatg gaggcaggcg aaccatccac
     1561 aggtactagt atccttttca taggtaacct ccattcctta ttcataaact tcctataaat
     1621 tatatacccc aggggtccct tgttatgcaa tgacataatg ttgacaaatg gaagcccatg
     1681 ggagttaggg agacaaaaaa tagctaatta acaacatatg ttcaaatcaa atatgacctc
     1741 tttctcttgt gaaatggctc caattttggt attatattat agatgatggg ttttaatacc
     1801 cagtaagcta tatacataat cttattacat atgaaaagga gtagtgaaaa tgccaaagag
     1861 tagaggacta agttttatca ttatcgctct ggtatcggtt gcagtgctgt tcataggcgg
     1921 cagcatgatg aactcaggcg gtggagcatt tgctgaaata ccagacggta catacgaagg
     1981 aacaagtgat gcaggtatgt atcctggcct taaggtagca gtcacctttg caggtggaag
     2041 cattacaaat gtagaggtag ttgaacatga cgagacacct ggaatctcag atccagccat
     2101 tgagggagta cctgcagcca ttgtggcggc aaactccaca gaggttgatg tggtatcagg
     2161 agcaaccctt acatcaaatg caattattga agcagtaaac aatgcaatag cagttgcatc
     2221 aggaaatgca cctgcagttg tatacgaaga tggagtttac gaaggcacta gtgacaaggg
     2281 aatgcaccct ggacttaagg tttccgtaac tgttgaaggc ggaaagatca caaatgttga
     2341 agttgtcgag catgacgaaa cacctggaat tgcagaccct gcaatcgagg gagtacctgc
     2401 agcgatcgtc gaggctggtt caccaaatgt cgatgtggta tcaggagcca ctttgacatc
     2461 agaagctatt atggatgcgg ttcaaaacgc actttcaggc gggtccggag aagaggaacc
     2521 agctgaggag gagcctgcag ttgaggagcc ggatatttca acaat
//
LOCUS       DASRQF010000050         2323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1344513, whole genome shotgun sequence.
ACCESSION   DASRQF010000050 DASRQF010000000
VERSION     DASRQF010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344513"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VFD79_01310"
     CDS             <1..203
                     /locus_tag="VFD79_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HZH92713.1"
                     /translation="PDMAGTVDRPETITVTVMDEKGQEVKMKPSGYLARVFCHEIDHL
                     DGVLYTDKAKEVHENLAEDDEI"
     gene            203..1129
                     /gene="fmt"
                     /locus_tag="VFD79_01315"
     CDS             203..1129
                     /gene="fmt"
                     /locus_tag="VFD79_01315"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HZH92714.1"
                     /translation="MRIIYMGTPEFAVESLERIHSEGYDIPLVVTQIDKKRGRGKKLQ
                     PTPVKEKALELGLEVFQPENINSDESFERLSALNPDLIVVAAYGQILKKRILDLPKYN
                     CINIHASLLPKYRGAAPINWAIANGEEKTGITIMNMEEGLDTGAMILKEEIPINWEDD
                     SQIIHDKLSSLGASMVVEAIELIESGKAEFVPQKHEESSYAPVLYKGVGNIDWKKTAN
                     EIYKKIMALKPWPSAFTEYKGQKIKIHRVEVEESEHEYTLGTIIDVDPEGIRVAVSDG
                     FIVIKELQFPGKRSMSVKELLLGNEIEKGYIF"
     gene            1145..1828
                     /locus_tag="VFD79_01320"
     CDS             1145..1828
                     /locus_tag="VFD79_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HZH92715.1"
                     /translation="MHTGFYGSSWMIFVLPAIIFAGYAQMKVKSTFSQYLKVPSESGM
                     TGAQVARNILDRNGLRDVPIEVVGGTLSDHYDPGKRVIRLSQNVYGGSSIASVSVAAH
                     EVGHAIQHAEGYFPLILRNNIAPIASFGSRLVWVFIILGFIISPFFIDVGIALFLAAV
                     LFQIVTLPVEFNASKRAMLSLEDGMIPPQSIKPARKVLNAAALTYVAATIVALAELLR
                     LLAISGRRR"
     gene            1865..>2323
                     /gene="nusB"
                     /locus_tag="VFD79_01325"
     CDS             1865..>2323
                     /gene="nusB"
                     /locus_tag="VFD79_01325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HZH92716.1"
                     /translation="MISVRDAAIRVLSEIDNEGKFSNKLLQQYSESELSKEDIRLLRE
                     LVYGVVENRMYIDKIIRSASSVKMKKIHPMILQILRLGVYQIGFMDRIPAHASINEAV
                     KLARKYGHKGTVGYVNGVLRRIEREREQHFSNDEPSSADDLAIRYSHPDYL"
BASE COUNT          730 a          399 c          581 g          613 t
ORIGIN      
        1 ttccagacat ggcaggcact gtggataggc ctgaaacaat cacagtcacc gttatggatg
       61 aaaagggaca agaggtaaaa atgaagccat cagggtatct ggcaagggtt ttctgccatg
      121 agatcgacca tcttgatggt gttttataca cagacaaggc aaaggaagtc cacgaaaacc
      181 tggcagagga tgatgagata taatgaggat aatctatatg ggtactccag aatttgcagt
      241 agagtcattg gaaagaatcc attcagaggg gtatgatatt ccccttgttg taacccagat
      301 tgacaagaaa agaggtaggg gaaagaagtt gcagcccacc cctgtcaagg aaaaggcact
      361 tgagcttggc ctagaggtat tccagcctga aaatatcaat tcagatgagt catttgaaag
      421 actatctgct cttaatccag atcttatagt tgtggcagca tatggtcaga tccttaagaa
      481 aaggattctc gacctgccta agtataactg tattaatata catgcttctt tactaccaaa
      541 gtacagagga gctgctccca taaactgggc tattgcaaat ggtgaggaaa aaacaggcat
      601 tacaatcatg aacatggagg aaggtctgga tacgggtgca atgattctga aggaagaaat
      661 cccaattaac tgggaggatg attctcagat aatacacgac aagctttcca gtctaggtgc
      721 aagcatggtc gtagaggcaa ttgagcttat tgagtcagga aaggctgagt ttgtacccca
      781 aaagcatgaa gagtcaagct acgcaccggt tctctacaag ggtgttggga atattgactg
      841 gaagaagact gcaaatgaga tatacaagaa aataatggcc ttgaaaccct ggccttctgc
      901 atttacggaa tacaagggac aaaagataaa gatacacagg gtggaagtgg aagaatcaga
      961 gcatgaatat accctgggaa ccatcataga cgttgatccc gagggtataa gggttgcagt
     1021 ctctgatgga tttatagtaa ttaaagagct gcagtttcca gggaaaagat ccatgtctgt
     1081 caaggaactg cttttaggta atgagattga aaagggatat attttctaga gaagagggat
     1141 gttcatgcat acaggatttt acggaagttc atggatgatt tttgtattgc cggcaatcat
     1201 tttcgctggt tatgcacaaa tgaaggtcaa gtcaactttt tcacagtatc ttaaagtccc
     1261 cagtgaatct ggtatgactg gagcacaggt tgcaagaaac attttggaca ggaatggctt
     1321 aagggatgta ccaatagagg tggtaggagg aacactatcc gaccactacg accctggcaa
     1381 gagagtgatt agactttcac agaatgtata tggtggcagc agtatagcct ctgtaagtgt
     1441 agcagctcat gaggttggac atgccattca acatgctgag ggttattttc cattgatact
     1501 caggaacaac atagctccaa tagcgagttt tggttctaga cttgtatggg tattcatcat
     1561 actgggcttt ataatcagtc ctttcttcat tgacgttggg atagcattat ttttagctgc
     1621 agttttgttt cagattgtta cactgcctgt agagttcaac gccagcaaga gggccatgtt
     1681 gagcttggag gatgggatga tcccacctca gagcataaaa ccagccagga aggtcttgaa
     1741 tgcagctgca ttgacatatg ttgccgctac tattgtagcc ttagctgagc tgctgagatt
     1801 attggcaata agtggaagaa ggagataaag gggggattga tccccctttt tttggagggt
     1861 ttaaatgatt agtgtacgag atgcagccat aagagtgctg tcagaaattg ataacgaagg
     1921 caaattttcc aataaactac ttcagcagta ttctgagtct gaactttcca aggaagatat
     1981 aagacttctt agagaactgg tatatggtgt tgtagaaaac aggatgtaca ttgacaagat
     2041 cattaggtct gcgtccagtg taaaaatgaa aaaaatccat cccatgattc ttcagatatt
     2101 aagactggga gtatatcaga ttggcttcat ggataggata ccagctcatg cttcaattaa
     2161 tgaagctgtt aaattggcga ggaagtacgg acacaaggga acggtagggt atgtgaatgg
     2221 agttttaaga agaattgaga gggaaaggga acagcatttc tctaatgatg aaccatcatc
     2281 ggccgatgat ttggccatta gatattctca tcctgactac ctg
//
LOCUS       DASRQF010000051         9308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_839549, whole genome shotgun sequence.
ACCESSION   DASRQF010000051 DASRQF010000000
VERSION     DASRQF010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 9308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9308
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839549"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1524)
                     /locus_tag="VFD79_01330"
     CDS             complement(<1..1524)
                     /locus_tag="VFD79_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92717.1"
                     /translation="MNNFKSAISGVFKRAAEAFQTYPAAMGNAFLFALVSIVKINMDW
                     EVQQDYSILLNSLQWGMAVGAIWSLAAITGANSRFNTTKAFIGANIFGFAATGITFIL
                     LFLFGESGLDRDYSYLSSIAQSRTSVAIAIGIVVFVYLASFPKEESDFSRAFFMTHKA
                     FFVAALYGMVLMMGASGVAGAIQALLYNDMSNKVFQYIAVVAGFMTFSIFIGYFPDFR
                     MGVKDPRRHELQDQPRFVQVLLGSILVPILLALTLVLLLWTVRTVLSGVESSFVRLSG
                     IAASYALGGIWLHIMLTRQDAGLVNFFKKVYPFTALVILAFEAWALIAQLNDTGLKTT
                     EYNFIMVWVFTVVSVLLLIFMKQESHRKIALMGCLIAAIAVLPVVGYHVLPVKYQVNR
                     LERVLLDEGMLQDGKVVPAASEPVREVREDITDAVSFLAFRESNNLPVWFSDNIYNDS
                     EFEDTFGFKKTWPEPDYEIPREYLSTNLVLQSGFVEIDEYDWAISFRDYYGSEAVSTE
                     "
     gene            complement(1642..3555)
                     /locus_tag="VFD79_01335"
     CDS             complement(1642..3555)
                     /locus_tag="VFD79_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZH92718.1"
                     /translation="MKKLIFVLLILTIVISSTQAIAAPSQWAVAEVQKARENNLVPAN
                     LLGDYQKNITREEFSEMAVRLHEALSGEISRNSSENPFTDTSNPEVLKAYELGIVKGF
                     GDGTFRPGKTITREEIAALLFRTLEKTNPEVTQGTYTIGFADRGQISIWFRNEVGFMS
                     KNGIINGIGSNTFAPKLETTREQAIALVVRLFSQYGGEGKLTAEQIGNLSAGAVQVFT
                     QYDNGDFGYGSGFFLAPGKIATNYHVIEGADEITIEYEDGTVYRGDIRVAGFDSGLDL
                     AVLSLEDTSVKPIQLGDSSGLVKGQKVYSISSPIGYKNILTEGIVSGATMDEIQVTIP
                     LSQGSSGGPLIDEYGRVVGVVFARMYGVESLGFAKPINSLKSMDIGKSLTIEEFNRAE
                     EILNPPINITARQKSAQEVSLSWDWMEADYFLIFKSIDGGESFQRVLNKSGNDRWEYS
                     NELTIPLDDYEEGTRIMFSVSSVKGELVSNRNHSNYVSLFKGMSESEIFDDLILNLPS
                     ISAEGTLINFEGYDVQRTGDNKITRIYAFVNEDGFEEMLKGDSDVVRGIATRLRTVSV
                     RYAELIQTDVELTVLYLGLYDEYPHFLEDNYINPDGIRRDQFIGLWEAEYPLINVANN
                     SGVYITWYGPYSF"
     gene            complement(3771..4229)
                     /locus_tag="VFD79_01340"
     CDS             complement(3771..4229)
                     /locus_tag="VFD79_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92719.1"
                     /translation="MSALERINESINIIEKDHKGQTLRKLDTKLLRRIALRFDEFSDD
                     CNECKTMSKDLDQYLKRLKDMKGEIAKDDLKDHFKKKSKYVAHLNKNHGLIQMGQNTG
                     LWMSLGVSIGIVLGLTTFDNFAMGLPIGISIGLLIGSSKDALAKKQDKVI"
     gene            complement(4276..4731)
                     /locus_tag="VFD79_01345"
     CDS             complement(4276..4731)
                     /locus_tag="VFD79_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92720.1"
                     /translation="MKASKNLQNNITKLSKSLKEKDKVVLDLDLFETMIIRLETLAGK
                     CQECRSLLDEAQPHFKDLADNNGIADRDMRKRQDNLRERMVKHLRKSHGLYRAGHFMS
                     IYLPLGISIGVTLGLVYSRLLYYSVAGAAFGYLIGLLVDSFVMKKKSRI"
     gene            complement(4764..5585)
                     /locus_tag="VFD79_01350"
     CDS             complement(4764..5585)
                     /locus_tag="VFD79_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92721.1"
                     /translation="MSDANNQKEITKLLDEYNQLRKKTAKAIVKRDRMSYHTYVDYKT
                     MYVTEIGELEYEFLKVENDLLKMRRKVEMIHSLMDNDEKIDISLIEHMLMKEFQDKDI
                     KLKRMEQDIEIASILRDDERLSRKDENLMDKLYEEMILELHPDINAEVTPDQDLDWEK
                     IVWTYETSDLEAMVTLRELLDEGRPSYGLMEVDSYKLEDRVEALKETLRRVKSEIEEM
                     KTIFPFDVVDLLKDEKWLDEKKKELKENTEIYRDVEEELGNYLLKILPQNAIFKN"
     gene            5737..6438
                     /locus_tag="VFD79_01355"
     CDS             5737..6438
                     /locus_tag="VFD79_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92722.1"
                     /translation="MKKYMVIVGFILVLSMLFVGCSQETPADTEPAAVEEENAETPVT
                     ESPTEEEQQGEQEVNLLSSAYAEVMMSNSYTMKYRTITSIDGQEFEATITTAVDGDNF
                     ATVFDSELAKSTTIQMDGKLYMVMHEQKMVMIFPADTDQAAEFESNENEVLETDGMEY
                     SQSGTAEFMGEIRKFEEYRVEDGTIRYYFDSENLVGIEMKGTDHESVWYIEEFLDSVD
                     MSLFEIPEDYTTFEP"
     gene            complement(6463..7533)
                     /locus_tag="VFD79_01360"
     CDS             complement(6463..7533)
                     /locus_tag="VFD79_01360"
                     /inference="COORDINATES: protein motif:HMM:NF019450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HZH92723.1"
                     /translation="MVRLAGIKSLSMILSMMIIFNTGIYTTEATGDLYQVKGYSEDVA
                     DFKVSNVVETFDSTTIDKAFDAGLIAELPVEIVVENNGGSSFGAYRLERSESGLYGYN
                     PDAQLTVIGEVSTMIPIEGAKKNIFGMYNEDQLEQVTLKIDDLEDHEYQDMEYLPGAK
                     IIIDQPGDYYIMFRIKATMGATEAFLRVVDSVEDGQAVENQEEVAEVVEAIPSNVSIL
                     VDGTDISFEAYNIGGNNFIKLRDLAFSLNQSNKSFEVGWSEEDRTIEIMEKRPYTTVG
                     GEMGTRVSTDNKGTINKDPVLLNGEEVDLLAYNIGGNNYFKLRDLAEKIDFGVTWDGQ
                     SRTIEINTENSYVEEIPQKQIF"
     gene            complement(7642..8100)
                     /locus_tag="VFD79_01365"
     CDS             complement(7642..8100)
                     /locus_tag="VFD79_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HZH92724.1"
                     /translation="MSIYKEIRTDLWELASNLDRHTRKKMELQVYSNIVNRMEHLCND
                     YNECSIFLEDMSSTIKEIKEKEGDLKREDIRAMKKVRKDVESYLEDEHDLIRKGKFMF
                     RYMTVGAAAGALVGYLFMSFMPVGVLLGALVGIGIGTKLDRDAKNEGVQI"
     gene            complement(8280..9227)
                     /locus_tag="VFD79_01370"
     CDS             complement(8280..9227)
                     /locus_tag="VFD79_01370"
                     /inference="COORDINATES: protein motif:HMM:NF016312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HZH92725.1"
                     /translation="MDIKKAVYYLATEGNIDHVASRVYEELEKNLQMKELDLVVDKKP
                     VMRYVDEGNNIFDFVRTDRVVCHDYRSYLLIMKESFSDYNVAGLITWHEGENAPDGIL
                     SVHTTGDVETGNFGRANPLFMHNILVALEKNRKEEGLKDFFVTTEATHWSGIVYGDMG
                     PEAILEYEVPIMDIEIGSSEKSWSNKLAVKVLAMSLLEIFRNDNLELKSILCTGGKHF
                     ERGFSAELFNRWEDKGLGISHIIPNQWMVSGEYEKESGMGKLRACVDSIEGGVQGIAM
                     HDGLKGAYKQQLRNLGEELGIPVFKHQFLRKPEEIKWSR"
BASE COUNT         2594 a         2241 c         1526 g         2947 t
ORIGIN      
        1 ctctgtactt accgcttcac ttccgtaata atccctgaaa cttatcgccc agtcatactc
       61 atctatctct acaaagcctg attggagaac aagattcgtg ctaagatact ctcttggtat
      121 ttcataatcc ggctctggcc atgttttctt gaagccaaag gtatcctcaa attcactgtc
      181 attataaata ttatcactaa accatacagg aagattatta ctttccctga atgccaggaa
      241 gcttacagca tctgtaatat cctccctgac ttccctgact ggctcagaag cagctgggac
      301 aaccttacca tcctgtagca ttccctcatc caatagaacc ctctcaagtc gattaacctg
      361 atacttgacc ggcaggacgt gatatcccac tacaggtaaa actgctatgg ctgcaatcag
      421 acatcccata agtgcaattt ttctgtggga ttcctgtttc atgaaaatca gtaacagtac
      481 agaaactaca gtaaagaccc aaaccattat aaaattatac tcagttgtct tcaaacctgt
      541 atcatttaac tgagcaatca atgcccaggc ctcaaatgcc aaaataacca aggctgtaaa
      601 aggatatacc ttcttaaaga agttcacaag acctgcatct tgccttgtca gcattatatg
      661 aagccagata cctcccagag catagcttgc agctatgcct gatagcctta caaatgaaga
      721 ttcaactcca gatagtacag ttcgaactgt ccatagcagc aaaacaagtg tcaaggccag
      781 cagtatgggt accagtatag atccaagcag tacttgtaca aaccttggct ggtcctgaag
      841 ctcatgtctt ctggggtctt tgacacccat tctgaaatca ggaaaatagc caatgaaaat
      901 actgaatgtc ataaaacctg ccactactgc aatatattgg aatactttat tgctcatatc
      961 attgtatagg agtgcctgta ttgctccagc cactccagat gcccccatca tcaggaccat
     1021 tccatacagg gctgcaacaa aaaatgcctt gtgggtcatg aagaatgccc gtgaaaaatc
     1081 tgactcctcc ttggggaatg atgccagata aacaaatact acaataccta ttgcaattgc
     1141 aacggatgtt ctagactggg ctatggaaga gaggtagctg taatctctgt ccaacccact
     1201 ttccccaaat agaaacagga ggataaaggt aattcctgta gctgcaaatc caaaaatatt
     1261 ggcaccaata aaggccttag tggtattgaa tctactattt gcaccagtga ttgccgcaag
     1321 gctccaaatt gcaccaactg ccatgcccca ctgcaaagag ttaagcaaga tgctataatc
     1381 ctgttggact tcccaatcca tattgatttt tacaattgaa accaatgcaa acaaaaaagc
     1441 gttgcccatg gctgcaggat atgtctgaaa agcttcagca gctcttttaa atactcctga
     1501 aattgctgat ttaaaattgt tcattccaaa aacctccaga cctggtctgt aattctttac
     1561 accttattta tacccatctc aaaacaaagt tgagaaacat ccgcaataaa tgcagtgaag
     1621 accactgaat ttcatatgct cctagaagct atatggtcca taccatgtaa tataaacccc
     1681 agaattgttt gcaacattta taagtggata ctcagcctcc cataggccta tgaattgatc
     1741 ccttcttatt ccatctggat ttatataatt atcctcaaga aaatgagggt attcgtcata
     1801 cagacccaag tatagaacag tcaattcaac atcagtctgt ataagttccg catatctcac
     1861 actaaccgtt cttagtctgg ttgcaatccc tcttacaaca tcagaatccc ctttaagcat
     1921 ttcctcaaat ccatcctcat tgacaaatgc atagattctg gttatcttgt tgtcaccagt
     1981 cctttgaaca tcataacctt caaagtttat cagggtacct tccgcactta ttgaaggtag
     2041 attaagaatc aagtcatcaa atatctcact ttctgacata cccttgaaaa gagatacata
     2101 gttggaatga tttctattag aaaccaattc acccttgact gatgatactg aaaacatgat
     2161 ccttgtacct tcttcataat catcgagagg aatagtgagc tcattgctgt actcccacct
     2221 gtcatttcca cttttattga gaaccctctg gaaactttcc ccgccatcaa ttgatttgaa
     2281 tatcaagaag tagtctgcct ccatccaatc ccagcttagg gagacctctt gggcactttt
     2341 ttgcctagcg gtaatattaa taggtggatt cagtatctct tcagccctgt taaattcttc
     2401 aattgtgagg cttttaccta tgtccattga tttaagacta tttatgggct ttgcaaagcc
     2461 caggctttcc actccataca tccttgcaaa tactacacca acaaccctgc cgtactcgtc
     2521 aatcaacgga cctccactgc tcccctggct aagagggatg gttacttgaa tctcatccat
     2581 ggttgctcca ctgactatac cctcagttaa tatattctta tagccgatgg ggctgcttat
     2641 tgagtaaacc ttctgtccct taaccagacc tgatgaatcc ccaagttgaa tgggcttaac
     2701 acttgtatcc tccagtgaca aaacagccag gtcaaggccg ctgtcaaaac cagcaactct
     2761 aatgtcaccc ctataaactg ttccatcctc gtattctatt gtaatctcat cagcaccctc
     2821 tattacgtgg taattagttg ctattttccc tggagcaagg aaaaaaccgc taccgtaacc
     2881 gaagtcacca ttgtcatact gggtaaagac ctgtacagca cccgcggata ggtttcctat
     2941 ctgttcagct gtaagtttcc cctccccacc gtactgacta aacaatctaa caacaagagc
     3001 aatagcctgc tcccttgtgg tttcaagctt tggggcaaat gtatttgacc cgattccgtt
     3061 gattattcca tttttactca taaagcctac ttcatttcta aaccatatgc ttatttgacc
     3121 cctgtctgca aaacctatgg tgtaggttcc ttgtgttact tccgggtttg tcttttcaag
     3181 ggttctaaat aacaatgctg ctatttcttc ccttgtaata gtttttcctg gcctgaatgt
     3241 cccatcccca aagcccttaa caataccaag ttcataagcc tttaagactt ccgggtttga
     3301 agtatccgta aaaggatttt ctgaactatt acgtgagatt tcacctgata gggcctcatg
     3361 taatcttaca gccatttcag agaattcctc ccgggtaatg tttttctggt agtcgcccag
     3421 tagatttgca ggtacgagat tattttccct tgccttctgg acctctgcta cagcccattg
     3481 actaggagct gcaatggctt gtgtagaact aatgacaatt gtaagtatca ataaaacaaa
     3541 tattaacttt ttcatttaag cacctcttca taattttatc cccaatggtg tatatccaca
     3601 gcggaacgat tccatccaaa aaaaaggagt acttgggact tcatcatcta ttcccattat
     3661 actccattat cacacctaat attcgcattt gaacaaattt taaccgcatt gtaatctttg
     3721 ttctattttc tacttgaaca gctgcttcct tggtccaagt caataaatga ttaaataacc
     3781 ttatcctgct ttttagccag tgcatccttg gacgatccga ttaatagacc aatggatatt
     3841 cctatgggca gccccatagc aaagttgtca aaagtggtta ggccaagtac aataccgatg
     3901 ctcactccca gggacatcca aagaccagtg ttctggccca tttgtatcaa cccatgattt
     3961 ttgtttaaat gggcaacata cttgcttttc tttttaaaat ggtcctttag atcatccttg
     4021 gctatctcgc ctttcatgtc cttcagcctt ttcaggtatt ggtccaggtc ctttgacatg
     4081 gttttgcact cattgcaatc atccgagaac tcatcaaacc ttagggctat tcttctaaga
     4141 agctttgtat caagctttct tagggtctgg cccttatggt ccttctctat aatattaatt
     4201 gattcattaa ttctttcaag tgctgacatt tacttgcctc ttttctcttt gataattgtt
     4261 caaatctatt taaatctaaa tcctgctctt tttcttcatg acaaagctat caaccagaag
     4321 accaattaaa tacccaaagg ccgccccggc aacagaatag tataggagtc tgctgtaaac
     4381 aagtcccaaa gtcacaccta tggaaattcc caatggaagg tagatgctca tgaaatgacc
     4441 tgctctgtaa aggccgtggc tctttcttaa atgctttacc attctttccc taaggttatc
     4501 ttgccttttc ctcatgtccc tgtctgcaat accattgttg tctgccaggt ccttgaagtg
     4561 tggctgggct tcatccaaaa ggctcctgca ctcctgacat tttcctgcaa gggtttccag
     4621 tctaataatc atggtttcga acaagtccag gtctagaact accttgtcct tctcctttaa
     4681 ggatttagat aacttggtaa tattgttctg taagtttttc gaagctttca tcgactctac
     4741 ctccataaat taggttaaaa gtatcaattt ttaaaaattg cattttgagg gagtatctta
     4801 agcaagtaat tgcctaactc ctcctccaca tccctataaa tctctgtgtt ttcctttaac
     4861 tctttctttt tttcatctaa ccatttctca tccttaagca ggtcaaccac gtcaaagggg
     4921 aaaattgttt tcatttcctc gatttcagat ttaaccctcc ttaaggtttc ctttagtgcc
     4981 tccaccctat cctccaactt gtatgagtca acttccatca aaccgtaaga tggtctgccc
     5041 tcatccaaca actccctgag ggttaccatg gcttcaaggt ctgatgtctc atacgtccaa
     5101 actatctttt cccagtccaa gtcctggtct ggtgtgactt cagcattgat atccggatga
     5161 agttccagaa tcatttcctc atacaattta tccatgagat tctcatcctt cctggacaac
     5221 ctctcgtcat ctcttagaat acttgcgatt tcaatgtctt gctccatcct tttcagcttt
     5281 atatccttat cttggaactc cttcatcaac atgtgttcaa taagggatat atcaattttc
     5341 tcatcattat ccatgagact gtggatcatt tcaacctttc tacgcatttt tagcaggtca
     5401 ttctccacct tgagaaattc atattccagc tcccctattt cagtgacata catggttttg
     5461 taatcaacat aggtatggta gctcatgcga tcccttttca ctattgcttt tgctgtcttt
     5521 tttctcaatt gattgtattc atcaaggagt ttagttattt ccttttgatt gttggcatca
     5581 gacaagttct caccttcttt caaagccttc tttaccttaa ttataccaca tgacttggga
     5641 caaatcacgt ctggacttga ctgctttgct tatgttatat cccatggggt atatagtttt
     5701 tgaaagtcga tatcatttgg gaaggaggga taagaagtga aaaaatatat ggttattgtt
     5761 ggattcatac ttgtactttc gatgttgttt gtcggatgta gccaggaaac tcccgctgat
     5821 actgagccgg cagcagtgga agaagaaaat gctgaaactc ctgtaaccga gtctcctact
     5881 gaggaagagc aacagggaga gcaagaggta aatctgttgt catctgccta cgcagaagta
     5941 atgatgagta acagctacac aatgaagtac cgtactatta ccagtataga tggtcaagaa
     6001 ttcgaggcaa caataactac tgcagtggac ggagataatt ttgcaaccgt atttgactca
     6061 gagctggcta agagcacaac aatccagatg gatggcaagt tatatatggt tatgcatgag
     6121 caaaaaatgg taatgatatt tcctgccgat acagaccagg ctgccgagtt cgaatcaaat
     6181 gaaaatgagg ttttagaaac cgatggtatg gaatactcac aaagtggaac agcggaattt
     6241 atgggtgaaa tcagaaaatt tgaggagtac agggtggaag atggaacaat tagatactat
     6301 tttgacagtg aaaatctggt tgggattgaa atgaagggga ctgaccatga gtcggtttgg
     6361 tatattgaag aatttttaga ctctgtagac atgagccttt ttgaaattcc tgaagactac
     6421 actacctttg aaccctaaag atttatacca caaaagcaga tcttaaaaaa tctgcttttg
     6481 tggtatttct tctacatatg agttttctgt atttatttct attgttcttg attgaccatc
     6541 ccaggtcaca ccaaaatcaa tcttctccgc tagatctctc agtttaaaat agttgtttcc
     6601 accgatatta tatgccagaa gatcaacttc ctcaccgttt aataaaactg ggtctttgtt
     6661 aatagttccc ttgttatccg tagaaactct tgttcccatt tcacccccta cggtggtgta
     6721 aggtcttttc tccattattt caatggttct atcttcctca ctccagccga cttcaaagct
     6781 tttattggac tggttaaggg aaaaagcaag gtccctaagc ttaatgaaat tattgccacc
     6841 tatattatag gcttcaaagg aaatatctgt accatccact aaaatcgaaa cgtttgaggg
     6901 tattgcttcc acgacttccg caacttcctc ctggttttca acagcttgtc catcttccac
     6961 cgaatcaacc actcgcagga aggcctctgt tgctcccatt gttgctttta tcctgaacat
     7021 tatatagtag tcacctggct ggtcaatgat tatttttgca ccaggaagat actccatgtc
     7081 ttgatattca tgatcttcaa ggtcatctat ctttagcgtt acttgctcca actggtcttc
     7141 attatacatt ccaaaaatat tcttcttggc tccttcaatt ggaatcattg tggagacctc
     7201 tccaatgacc gtcagctgtg catcaggatt ataaccatac aatccacttt cagacctttc
     7261 aagtctgtag gcgccaaagc ttgatccacc attattctcc accactatct caacaggaag
     7321 ttctgcaatc aaaccggcat cgaaggcctt gtcaatagta gtcgagtcaa aggtttccac
     7381 tacatttgaa accttaaagt ctgccacatc ctcagaatac cctttcacct ggtaaaggtc
     7441 tccggttgct tctgttgtgt aaatacccgt gttgaaaatt atcatcatgg ataaaatcat
     7501 gctcaagctt tttattccag caagtcttac catattgaac actcccttat gtagtcagtt
     7561 actgtactcc aattataaca gaacaagctg gtggtttgtc aaaaaaataa agccggaaat
     7621 aaatccggcc atgtaaattc attaaatctg aactccttca ttttttgcat ctctatcaag
     7681 cttggtacct attccaattc caaccaaagc ccccaagagt accccaacag gcatgaaact
     7741 cataaagagg tatcccacaa gtgcccctgc agctgctcct actgtcatgt atctgaacat
     7801 gaattttccc tttctaatca ggtcatgctc atcttccaag taggactcaa catccttccg
     7861 tacttttttc atggcacgga tatcttccct cttaaggtca ccctccttct ctttaatctc
     7921 cttgatcgtg gaggacatgt cttccaggaa gatactacat tcgttgtaat cattgcataa
     7981 atgttccatt ctattcacta tatttgaata aacctgcagc tccatcttct ttctggtgtg
     8041 cctgtccagg ttacttgcca attcccataa atccgtcctg atttccttgt atatactcat
     8101 cacatcactc tccctaacca tttttaaagt cttttcattt tacttgtgaa gtatataccc
     8161 agttaatatt catttctatc tatatatggg atattttttt gcaagttgtt atattatcaa
     8221 atactgttaa tatcttgtca ctgctgatct ttaaccttag ctaccgatga tacacctccc
     8281 taccggctcc actttatttc ctcaggtttt ctgagaaact ggtgcttgaa aacaggaatt
     8341 cccaactcct ctcctagatt tctcaactgc tgtttatatg ctccctttaa tccatcgtgc
     8401 atggcgatgc cttgaactcc gccttcaatt gagtcaacac atgcccgcag ctttcccata
     8461 ccagattcct tttcatattc acctgatacc atccactggt tcggtataat atgagaaatg
     8521 ccaagtcctt tgtcttccca tcgattgaac agttcggctg aaaaacctct ctcaaaatgc
     8581 tttccccctg tgcatagaat actcttcagt tccaaattgt cattcctaaa aatctccaac
     8641 agggacattg ccagaacctt tactgcaagc ttattgctcc agctcttttc actactacca
     8701 atctctatat ccataatggg aacctcatat tccaaaatag cttcagggcc catgtcacca
     8761 tatacaatcc cggaccaatg ggttgcctcc gttgttacga aaaaatcttt caacccttcc
     8821 tctttcctgt tcttttccag tgcaaccaat atattatgca tgaataacgg attggccctt
     8881 ccaaaatttc cagtttccac atccccggtt gtatgtaccg acaaaatacc atcaggtgca
     8941 ttttctccct catgccaagt tatcaagcca gccacattgt agtcactgaa gctttccttc
     9001 atgattaaca ggtagcttct atagtcatgg caaacaaccc tgtctgtcct tacaaaatca
     9061 aatatattgt tgccctcgtc cacatatctc ataacaggct tcttatccac tacaagatcc
     9121 agctctttca tttgaagatt cttttcaagc tcttcataca ccctgctggc aacatgatct
     9181 atatttcctt ctgttgcgag atagtatact gcctttttaa tatccatgct agacatcctt
     9241 tctattagtc aagtccaatt gatttgcaat tcattacttt catttgagct taaccataac
     9301 cccatcct
//
LOCUS       DASRQF010000052         2029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_957489, whole genome shotgun sequence.
ACCESSION   DASRQF010000052 DASRQF010000000
VERSION     DASRQF010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957489"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..671)
                     /locus_tag="VFD79_01375"
     CDS             complement(180..671)
                     /locus_tag="VFD79_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZH92726.1"
                     /translation="MFREMRRKKQLLSQEGMELVMARGTNGVLACQGDEGYPYAVPVS
                     YVYWNDRIYFHSAKAGHKVDAIRKDSKVSFTVVDEDTIVSEEYTTYFRSVIAFGKARI
                     VEGDEWFEGFKALVDKYSGDLPEDANIKEIQGCKTSLIIAIDIDHITGKEAIEYVRAK
                     QGK"
     gene            complement(817..1137)
                     /locus_tag="VFD79_01380"
     CDS             complement(817..1137)
                     /locus_tag="VFD79_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92727.1"
                     /translation="MKNEEFQRIVLEELRDLKVGQGRLEVRMDKIEDKVGSLEDRMGS
                     LEDRMGSLESKLDAVVEQTAILTEFKEDTSKKLGIIIEENKVFKEIIGRHEVDIEMLK
                     KKIC"
     gene            complement(1265..>2029)
                     /locus_tag="VFD79_01385"
     CDS             complement(1265..>2029)
                     /locus_tag="VFD79_01385"
                     /inference="COORDINATES: protein motif:HMM:NF021498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wadjet anti-phage system protein JetD
                     domain-containing protein"
                     /protein_id="HZH92728.1"
                     /translation="NRSVRFFSGDYEEFERILMAVRELENLHVEQFIREFSVRLYKDS
                     KAFGQIQGKVESILYDYGDFPEKDEILGNLNLMRTPTYVNFKGSGKVSLSGQEIDLSR
                     LDSDIAISSSMLDKIESVQVFKGKVITVENLTSFHRFMEEGYFVLYLGGFHNRVRRDF
                     IRMLHDQNSEARFYHFGDIDAGGFLILEHLRRTTGIKIIPYNMNLDTLKKYEKMGKPL
                     TENDRKRLGKLREGQFREIVEYMLENNVKLEQEAIV"
BASE COUNT          529 a          544 c          298 g          658 t
ORIGIN      
        1 ttttatcaag ctcatctatc tggtctataa attccttgtg agtgttcatc ctaaatccct
       61 cctttatgct ttgatacccg taacgaccgg tcattctaac atataccgat taaagacata
      121 tgctcaccta ttgtctttgg acaaagaaaa aagcgggctt ccccgcttga taattgaatc
      181 tattttccct gttttgccct cacatactca attgcttcct taccggtgat atggtcgatg
      241 tctatggcaa ttatgagtga tgtcttgcaa ccctggatct cctttatgtt tgcatcctcg
      301 ggaaggtccc ctgaatactt gtccaccagg gccttaaagc cctcaaacca ctcgtcgccc
      361 tctacaatcc tggcctttcc aaatgcaatg acacttctaa aatatgtagt gtactcctcg
      421 cttacgatgg tatcctcatc aacaacagta aaggatacct tggaatcctt tcttatggca
      481 tccaccttgt gacccgcttt tgctgaatgg aaatatatcc tgtcattcca gtatacatag
      541 cttacaggaa ctgcataggg ataaccctca tcaccctggc aggcaagtac tccatttgtt
      601 cccctagcca ttacaagctc catgccctcc tggctcaaaa gctgcttttt tcttctcatt
      661 tccctgaaca ttcataactc cccctaaaat ctattttgac ctctttccaa tctatattta
      721 cttgtcctgg atagcaaggg aaccacagcc cttcatcgat accattttat cacattaccc
      781 accaaaaaac ccaccacaat tgtggtgagt ccttatctag catatttttt tcttaagcat
      841 ctctatgtca acttcatgcc ggccgattat ttccttgaaa accttgtttt cttcaatgat
      901 gatgccaagc ttcttactgg tatcttcctt gaactcagta agtattgctg tctgttctac
      961 aacagcatcc aatttgctct caaggcttcc cattctgtct tcaagacttc ccattctgtc
     1021 ttcaagactt ccaaccttgt cttcaatctt gtccattcgg acctcaagtc ttccctgacc
     1081 taccttcaga tcccttaact cctccagcac aatcctttga aattcttcgt tcttcatatg
     1141 gacacctccg aatcaatcat ccgtatggat attataccac aaatcccata cgaaattatt
     1201 aagtatattt tagcataact taacatttcg tacaagtgtt cgatttattt tttttgaggt
     1261 ttcactacac aattgcttcc tgttcaagct ttacattatt ctccagcatg tattcaacta
     1321 tttcacggaa ctgcccttct cgaagcttcc caagcctttt cctgtcattc tccgtaagag
     1381 gctttcccat cttttcatac tttttcaggg tgtctaaatt catgttgtag ggtattatct
     1441 taatcccagt ggttctccta agatgttcca gaataaggaa tcctcccgca tcaatatcac
     1501 caaaatgata aaatctcgct tcactgttct ggtcatggag catccttatg aaatccctcc
     1561 tgaccctgtt gtggaatccg cccaggtaca atacaaaata accctcttcc atgaacctat
     1621 gaaaggatgt cagattctca actgtaataa cctttccctt aaacacctgc acggactcaa
     1681 tcttatcaag catgcttgaa gaaattgcaa tatccgagtc caacctgcta aggtcaattt
     1741 cctggccgga cagagagacc ttgcctgatc ccttgaaatt gacataggtg ggagttctca
     1801 taaggttgag atttccaagg atctcatcct tttcaggaaa gtcaccataa tcatacaata
     1861 tactctccac cttaccctgg atttgcccga atgcctttga atccttgtat agccgtacag
     1921 aaaactccct gatgaactgc tctacgtgaa gattttccag ttcccttaca gccataagaa
     1981 ttctttcgaa ttcttcgtaa tcaccagaaa aaaaccttac actcctatt
//
LOCUS       DASRQF010000053         6123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1856634, whole genome shotgun sequence.
ACCESSION   DASRQF010000053 DASRQF010000000
VERSION     DASRQF010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1856634"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VFD79_01390"
     CDS             <1..220
                     /locus_tag="VFD79_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HZH92729.1"
                     /translation="SIGTAGVPGAGAIMLTLVLQSVGLPLEGIGLIMGIDRLVDMART
                     AINVTGDAVCTTIIAKSEGGLNEDVFNA"
     gene            297..1286
                     /locus_tag="VFD79_01395"
     CDS             297..1286
                     /locus_tag="VFD79_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HZH92730.1"
                     /translation="MEIGKVPNELLEKIVFENISLRREEVLVRASIGEDTGVMDLGGD
                     LCVVSTDPITGATKEIGALAVHVSCNDAATKGAEPVGLLLTILCPEGTGEDELEEVMK
                     QAGRAAADVNVEIIGGHTEVTDAVTRIVLSTTVIARMKREDLHTYESIRTGDKVVISK
                     WIGLEGTHILASELKEILMDDFTQGEIQEAIDLGRHLSVVREGLLARKFGVKYMHDIT
                     EGGIHGAIWETGKAIGKGIDLFEEKLPVLDITRRICDHLQVDVRKLISSGSMIMVVPR
                     ERAHDLVQALEMVHIPASVVAEVTDSGIFSITDETRTQIESPGSDELYRALEV"
     gene            1399..3534
                     /locus_tag="VFD79_01400"
     CDS             1399..3534
                     /locus_tag="VFD79_01400"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HZH92731.1"
                     /translation="MRRTILTAIMIMLIVTLFSISFGNSSVDIDINGNRMALPQVAVV
                     MDGRPTDTEFPSFIMGDRTLVPIRFVAESFGAQVGWDPATRTASVTHQGNRLDLTIDS
                     NVVLVNGNPMTLEENSIPKLVVYTSMNEAKTMVPVRFVSEIMGYEVGWDAASYTALIN
                     SPKAEDKGDLVTISGISSERARNGNHRVKIETDGELKYTTMFLPASNKLVVDVENARL
                     DIEGSRDRPGDFFVEGDVLTRLQYSQFTINPYTTRIVMTLTEEEDFRLVQEDDQVYIL
                     FGNDRLEVPEEAEEPEDEEDEPEDEGKDDGPRMIRQEMVDGKNAIVVYGLDSEEYNVT
                     KLSDPTRLVIDIMDVKIVGELYQEYDYQLGFIKGVRASQYVGDRNYGPDRDVVRVVLD
                     VMEGAKDPGIIVLPERGRLVILPEENAWDALQYQNRGTESRFDFRNLFPTQYDVDYDE
                     MRKEIKIKLPVTATNLPDGTLQIGDQLVRDISVERGRNETEITIRFRRGVVYSVLSRP
                     QDQVISIQAIRNEEVLPQNTTIVVDAGHGGKDPGAVSGSTREKDINLQTAFKLRDALL
                     SRGYNVIMTRDTDVFIDLYSRPRIANENNADLFISLHANATLSSSINGLEILYCPSYG
                     SDKKIDDQFPFAEFVYNSILQQTGRTGRGIIKRPELVVLRETEMPAVLVETGYMTNPT
                     ELALVVSDHYQSQVVQGIANAVDNYFKEY"
     gene            3589..4110
                     /locus_tag="VFD79_01405"
     CDS             3589..4110
                     /locus_tag="VFD79_01405"
                     /inference="COORDINATES: protein motif:HMM:NF039198.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HZH92732.1"
                     /translation="MELLMGYLLIFFARMLDMSMATVRTLMVMQGRKFQSALIGFFEV
                     GIYVTVLGKVVNSLENPMNLLAYCLGFAAGNYVGIMLENKIALGNLSAQIILKTANND
                     ILIDTLRQEKFGVTSYEGQGIEGPREMLTVALNRKDLTRLKTIVDAIDPNAFITVNSI
                     SPIRGGYFTKMKK"
     gene            4211..4942
                     /gene="rph"
                     /locus_tag="VFD79_01410"
     CDS             4211..4942
                     /gene="rph"
                     /locus_tag="VFD79_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HZH92733.1"
                     /translation="MRIDNRKHDEMRPVNIERDYLLHPYGSVLIQMGDTKVICTAMVE
                     DKVPHFLKNTGTGWVTAEYSMLPGSTMTRKMRDSTKGRVDGRNQEIQRLIGRSLRSVV
                     DLKKLGERTVWIDCDVIQADGGTRTASITGAYIALVDALHKLYQKEDMKEFPITGYLS
                     AISVGVGEEGPILDLCYQEDHKAKVDMNVVMGDNGQFVEVQGTGETGTFSRKELDQLL
                     DLAEKGTGELIDLQKEILGPITELI"
     gene            4955..5554
                     /gene="rdgB"
                     /locus_tag="VFD79_01415"
     CDS             4955..5554
                     /gene="rdgB"
                     /locus_tag="VFD79_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HZH92734.1"
                     /translation="MRKLIISTDNENKAKELREILKDLPLEILTKKEAGIENLQVVED
                     GDTLEHNSLKKARELAGLVDYMVMADDSGLFVDALDGAPGVYSSRYGGEDGNDALNNQ
                     TLLKNLKGKERTAAFRTVIALVTEDGRETTVQGECRGKIMEEPKGENGFGYDPLFVPE
                     GYSETFAQLPIQVKNSISHRRRAIDALREKLEAILSEQH"
     gene            5541..6026
                     /locus_tag="VFD79_01420"
     CDS             5541..6026
                     /locus_tag="VFD79_01420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HZH92735.1"
                     /translation="MSSIKVIVVADTHHVTQPIIQALKNERDAQGFVFLGDHVEDGEK
                     IKNSLNLPSYIIGGNGDWSTYYKKEMILNLGGKRILITHGHRQGVKTGLQRLYYHALE
                     NSADAVLFGHTHMPEVRREGDLFMMNPGSPSFPRGGHTLGTYGILHIGEMITGEIKRI
                     R"
BASE COUNT         1860 a         1195 c         1675 g         1393 t
ORIGIN      
        1 atcaatcgga acagctggtg taccgggagc gggagcgata atgcttacct tggtgcttca
       61 atccgttgga cttcctcttg agggaatcgg actgataatg ggtattgaca gacttgttga
      121 catggcaaga acagccatca acgtgacagg tgatgctgtc tgtacaacaa taattgcaaa
      181 atctgaaggt ggattgaacg aagacgtatt caacgcctaa gagagggccc tgacttaagt
      241 cagggctttt aattttcccc tttttctgtt accattaggt aagggaggcg agaaggttgg
      301 agataggaaa agtaccaaac gaactactgg aaaaaattgt ttttgagaat atatccctaa
      361 ggagggaaga ggtcctggtt agggcaagca ttggtgagga cacgggtgta atggaccttg
      421 ggggagacct ttgcgtggtc agcacagacc ccataacagg ggctacaaag gaaattggag
      481 ccctggctgt acatgtttcc tgcaatgacg ctgcaaccaa gggagcggag cctgtgggac
      541 ttctcctgac catcctctgc cctgagggta ccggtgagga tgagcttgaa gaggtgatga
      601 agcaggcagg tcgggcagca gctgatgtga atgttgaaat cataggtggc cacactgaag
      661 ttacagatgc ggtaactaga attgtcctaa gcaccacggt gattgcaagg atgaagaggg
      721 aagacctcca tacctatgaa tccataagga ctggagacaa ggtggtcata tcaaagtgga
      781 taggacttga gggaacccat attcttgcca gtgaactaaa ggagatcctt atggacgatt
      841 tcacacaagg ggagatccag gaagcaattg atctggggag gcacctgagt gttgtaagag
      901 agggactcct tgccaggaag tttggcgtca aatacatgca cgacataaca gagggtggaa
      961 tccatggtgc catctgggaa acaggaaagg ccatcggcaa gggaattgac ctctttgagg
     1021 aaaagcttcc tgttctggat ataaccagga ggatctgtga ccaccttcag gtggatgtga
     1081 gaaagcttat ttcaagcggc agcatgataa tggttgtacc ccgggaaagg gcccatgatc
     1141 tggttcaagc ccttgaaatg gttcatatac ctgcaagtgt ggttgcagag gttacggatt
     1201 ctggtatttt cagcatcaca gatgaaacca ggacccagat tgaatcccct ggaagtgacg
     1261 agctttatag ggctttggaa gtgtagaaac ccatattaca ttctggttac attgtccccg
     1321 accatattgt atgaaggtct ataaaatgat ataaatatcc taggtagact tgtgtttttt
     1381 gaagcggggg agattcttat gagacgtact attttaacag caataatgat tatgcttatt
     1441 gtaaccctgt tttcaatttc ctttggaaac agcagtgtgg atatagacat aaacggcaac
     1501 aggatggcat tacctcaggt tgcggttgta atggatggaa ggccaacgga tacagagttc
     1561 ccatcattta tcatgggaga caggaccctt gtgccgatca ggttcgttgc agaaagcttc
     1621 ggagcccagg taggctggga tccggccacc aggacagcat cagtgaccca ccagggaaac
     1681 cgtttggacc ttacaattga cagcaatgtt gtacttgtaa atggaaaccc catgacactg
     1741 gaggaaaact ccattcccaa acttgtggta tatacctcga tgaatgaagc caagactatg
     1801 gtaccggtga ggtttgtttc agagataatg ggctatgagg tgggttggga tgcagccagc
     1861 tacacggccc taataaattc acccaaggct gaggacaagg gagacttggt gaccatctct
     1921 ggaatttcat ctgaaagagc tagaaatggt aaccacaggg tcaaaattga gaccgatggg
     1981 gagttgaaat acaccacaat gttcctccca gcttcaaaca agctggttgt ggatgttgaa
     2041 aatgcaagac ttgatattga aggaagcaga gaccgtccag gggacttctt tgttgaggga
     2101 gatgtcctta caagacttca gtattcacag tttacaatca acccatacac cactaggatt
     2161 gtcatgacct tgactgagga agaagacttc aggctggtcc aggaagatga ccaggtatat
     2221 atcctatttg gaaatgacag actggaggtt cctgaagaag ctgaggagcc tgaggatgag
     2281 gaagatgaac ctgaagatga aggcaaggat gacggtccaa ggatgataag acaggaaatg
     2341 gtggatggaa agaatgccat tgtagtctat ggacttgaca gcgaggagta caatgtcaca
     2401 aagctaagtg acccaacaag gcttgtgatt gatataatgg atgtaaagat tgtgggagag
     2461 ctatatcagg agtatgacta ccagcttgga tttatcaagg gagtcagggc atcacagtat
     2521 gttggtgaca ggaactacgg tcccgacagg gatgtggtca gagttgtact tgatgttatg
     2581 gagggtgcca aggatccagg gataatcgtt cttcccgaga ggggaaggct ggtgatccta
     2641 cctgaagaga acgcatggga tgcccttcag tatcagaaca ggggaacaga aagcaggttt
     2701 gacttcagga accttttccc aactcaatat gatgttgact atgatgaaat gagaaaagag
     2761 atcaagataa aactgcctgt aacagcgacc aatcttccag atgggaccct tcagattgga
     2821 gaccagctgg taagggatat ttcagttgaa cgtggaagaa atgaaactga aatcaccatc
     2881 agattcagaa gaggtgtggt ctatagtgta ctttctagac cccaggacca ggtaattagc
     2941 atccaggcca tcaggaacga ggaagtgctt ccacagaaca ccacaattgt agtggatgca
     3001 ggtcatggtg gcaaggatcc aggtgcagtg agtgggtcaa ccagggagaa ggacataaat
     3061 ctgcagacag ccttcaagct tagggatgcc cttctgtcaa ggggatacaa tgttatcatg
     3121 acaagggata ctgacgtctt tattgacctt tacagcagac ctaggattgc aaatgaaaac
     3181 aatgcggacc tgtttataag cctccatgca aatgcaacac tgagtagctc cataaacggt
     3241 ctggagatac tatactgtcc ctcatatgga agcgacaaga agattgatga ccagttcccc
     3301 tttgccgagt ttgtatacaa cagcatactt cagcagacag gaagaacagg aaggggaata
     3361 atcaagagac ccgaacttgt agtcctaagg gaaacggaga tgcctgcagt ccttgttgag
     3421 accggataca tgacaaatcc aacagagctc gccctggtgg tctcagacca ctaccagagc
     3481 caggttgttc agggaattgc aaacgcagtg gataattatt tcaaagagta ttgataaatg
     3541 gggaaggtta agccttccct tgcatttgtt tgaaaggagc atcatttgat ggaattgtta
     3601 atgggatatt tgctgatatt ctttgccagg atgttggaca tgtcaatggc tacagtaaga
     3661 accctcatgg taatgcaggg tagaaagttt cagtcggccc taatcggatt cttcgaggtt
     3721 gggatatacg taactgtcct tggaaaggtt gtaaactcac ttgaaaaccc tatgaatctt
     3781 cttgcatatt gccttggatt tgctgcagga aactatgttg gaatcatgct tgaaaacaag
     3841 attgcccttg gtaatctctc agcccagata atcttgaaga cagccaacaa cgatattctt
     3901 atcgataccc tgagacaaga gaagtttgga gtcaccagct atgaaggaca gggaatagag
     3961 gggccaaggg aaatgctgac agtggcactt aacaggaagg acctgaccag gctgaagact
     4021 attgttgacg ccatagaccc aaacgcattt ataacagtca acagtatcag cccaatccgt
     4081 ggaggatact tcacaaagat gaaaaaatga tttttgcccc tgtaaagggg tttttttctt
     4141 gccttcatgt atgaataagg gtcaatgttt gatagaatgg aagtagtaaa aatatagagg
     4201 agttgtttag atgagaatag acaataggaa acacgatgaa atgagacctg tgaatataga
     4261 aagagactac ctcctgcacc cctacggatc ggtacttata cagatgggtg acaccaaggt
     4321 catatgtact gcaatggtgg aggacaaggt cccccacttc cttaagaata ccggtacggg
     4381 atgggttaca gccgaatatt caatgcttcc agggagtacc atgactagga agatgaggga
     4441 ctcaaccaag ggtagggtag atggaagaaa ccaggagata caaaggctca tcggaaggag
     4501 cctaaggtct gtggtggacc tgaaaaagct gggagagagg accgtatgga tcgattgtga
     4561 cgttatccag gccgatggtg gcaccaggac agcctcaatt actggagcct atatagccct
     4621 tgtggatgcc cttcataagc tttaccaaaa ggaggatatg aaggaattcc caattacagg
     4681 ctacctttca gcgatcagtg ttggggttgg tgaggaaggg ccaattcttg acctgtgcta
     4741 ccaggaggac cacaaggcca aggttgacat gaacgtggta atgggggaca atggacagtt
     4801 tgttgaggtg cagggtaccg gggagaccgg aaccttctca aggaaggagc tggaccagct
     4861 tctggacctg gcggaaaagg gtacaggaga gcttatagac cttcaaaagg aaatccttgg
     4921 accaataaca gagcttatat agaggggtag aaatatgaga aagctaataa tttcaacaga
     4981 caacgaaaat aaggcaaagg agctgaggga gatcctaaag gaccttcccc tggagatact
     5041 gacaaaaaag gaagccggca tcgaaaacct ccaggttgtg gaggatgggg ataccctgga
     5101 gcacaacagc ctcaagaagg caagggagct tgcggggctt gtagactaca tggttatggc
     5161 agatgattcc ggactctttg tggacgctct cgacggggcc ccaggggtgt actcatcaag
     5221 gtatggaggg gaggacggaa acgacgccct caacaaccag accctcctaa agaacctgaa
     5281 gggcaaggaa aggacagcgg cctttaggac agtcatagcc ctggtaaccg aggatggaag
     5341 ggagaccaca gtccagggtg aatgcagggg aaaaatcatg gaggaaccaa agggagaaaa
     5401 tggctttggc tacgaccccc tttttgtacc ggaagggtat tctgaaacct tcgcccagct
     5461 gccaatccag gtcaagaact caataagcca caggagaagg gcaatagatg cattaaggga
     5521 aaaactggag gcgattctaa gtgagcagca ttaaggtaat cgtcgttgcc gacacccacc
     5581 atgtaacaca gccaataatc caggccttga aaaatgaaag ggatgcccaa ggcttcgtgt
     5641 ttctgggaga ccatgtggag gatggagaaa agattaaaaa ctctctgaac ctgccatcct
     5701 atatcatagg tggaaacggt gactggtcaa cctactacaa gaaggaaatg atattgaatc
     5761 tgggaggaaa gaggatactg atcacccatg gccacaggca gggtgtgaag accggcctgc
     5821 aaaggcttta ttatcatgcc cttgaaaaca gtgcagatgc tgtcctcttt gggcacaccc
     5881 atatgcctga ggtgagaagg gaaggcgacc tatttatgat gaacccggga agtccatcct
     5941 ttccaagggg tgggcacaca cttggaacct acgggatcct tcacataggt gaaatgatta
     6001 ctggagagat aaagagaatc aggtaggaga gatatatgta atggaaagag cactggactt
     6061 tctacatctt gatatggacg ccttctttgc agctgtggag gagaaggaca acctaaagat
     6121 aaa
//
LOCUS       DASRQF010000054         3642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1083143, whole genome shotgun sequence.
ACCESSION   DASRQF010000054 DASRQF010000000
VERSION     DASRQF010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083143"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1566)
                     /locus_tag="VFD79_01425"
     CDS             complement(<1..1566)
                     /locus_tag="VFD79_01425"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZH92736.1"
                     /translation="MEDYLLFIFYLSAIIYLEAALRQKFKDGQFPVGMRLPVVFYIPF
                     ASLFFLLSTSLSRGFNFWISLAFISITCILFASQLVYFYIFRTFYIVYSAGNAGDVME
                     FAGIAISTILDNLSKVLAIFLPLPVFILLSSLGLFSFTTDFWSRLIILAAGVASHILG
                     LHLVNIGSRSVNSPYNLYYNIHQPEFAVDKLGLLTYMRLDLKRQVFGWTPRHLPDEDS
                     LVIYMDSSTIGKSNNMDNKGFNMLPLDFKSLEMGESDEKIKQLHRYFSLMEPSEKNEY
                     TGMFKDHNLILITAESFSHLAIREDVTPTLYKMVHEGFNFTNFYTPIWGVSTTDGEYV
                     ATTGLLPKPGDWSYPRSKDNYMPFAMGNQLKGKGYLTKAYHDHSYDYYQRHLTHKNMG
                     YDYKGLGNGLDMDHTWPESDLEMMEKTIPDYIDHERFHAYYMTVSGHLIYDFKSNQMA
                     KKNRHLVQDLPYSNKVQAYLAGQIELDRALEYLLKRLEEEGVAERTLIAISSDHYPYG
                     LTLDEQEDLKGGRI"
     gene            1702..3000
                     /locus_tag="VFD79_01430"
     CDS             1702..3000
                     /locus_tag="VFD79_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HZH92737.1"
                     /translation="MAKQTIMVNELTNGVLDPKEPMLGPVRDGGTIIANTAPGCWGPM
                     ITPALRGGHEVTKPVFVEGAQVGDAIAIYIKSVAVTSSVVASGNEFTIPENFDGDPFV
                     AGKCPHCGAKHPDTVLEGIGDASVRCAHCGAEIAPFKFTHGYTMAFNEEKSVGLTLTK
                     EKAEEMAKKAKEVMAIPDASIQNPIVTFAPHDITGNISRMRPFIGQLGTTPSVAMPDS
                     HNAGDFGAFLIGAPHEYAIDNETLQRSKTDGHMDINRVRQGAVVICPVKVEGGGVYVG
                     DVHGMQGDGEIAGHTTDVSAIVTLKVKLLKGLKLDGPIILPLEEDLPHLARPITDAEK
                     TKAIKLGSSLGMDEYEETAPISFVGTGENLNVAIDNGLERAAAVLGITVPEVKNRTTI
                     NGGLEIGRYPGTATVTFLAPVDLLEKAGLLEMVKDQYNIK"
     gene            3058..3624
                     /gene="thpR"
                     /locus_tag="VFD79_01435"
     CDS             3058..3624
                     /gene="thpR"
                     /locus_tag="VFD79_01435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HZH92738.1"
                     /translation="MRLFICIDLGKDQLDIVEMLKKRLEGESIKGRFAKPEHMHLTLN
                     FIGEIEDEKLRDVIEVMDRVYFSGFDIHMSGLGWFHKRSGKIYWVGLERSEELFGLQE
                     DLHKALLDRGFNLEKRGYTPHITLGRAVRVVEGFDPSRYTELVQGTQIIVDKIVLKKS
                     ENTGSGVIHTILYSKAAKDSLRSKEERN"
BASE COUNT         1070 a          691 c          924 g          957 t
ORIGIN      
        1 aatccttcca cccttaaggt cctcctgctc atccagtgta agaccatagg ggtaatggtc
       61 tgagcttatt gcaataaggg tcctttctgc cactccctct tcctcaagcc ttttcagcag
      121 gtattccaaa gctctgtcca gttcaatctg ccccgcaagg taggcctgga ccttattgga
      181 atatggaagg tcttggacca ggtgcctatt tttcttagcc atttggttgc tcttgaaatc
      241 atagataagg tgtccgctta ctgtcatgta gtaggcatgg aacctttcat gatcaatgta
      301 atcaggaatg gtcttttcca tcatttcaag gtctgattca ggccaggtat ggtccatgtc
      361 caggccattt cccaggccct tgtaatcgta gcccatgttc ttgtgggtaa ggtgcctctg
      421 gtaatagtcg taggagtggt cgtgataggc ctttgtgaga tagccctttc ctttcagctg
      481 gttgcccatg gcaaagggca tgtagttgtc ctttgatctg ggatagctcc agtcgcctgg
      541 cttgggaagc agaccggtgg ttgcaacgta ttccccatcg gttgtactga cgccccagat
      601 gggggtgtag aagtttgtaa agttgaagcc ttcatggacc attttgtaaa gggtgggtgt
      661 cacgtcttcc cttatggcaa ggtgtgaaaa gctttcagct gtaatgagaa tcaggttgtg
      721 gtccttgaac atgcctgtgt actcattttt ttcagaaggc tccataaggg agaaatacct
      781 gtgtagctgt ttgatttttt catctgattc ccccatttcc aaagacttaa aatccagtgg
      841 aagcatattg aatcccttat tatccatatt attagacttg ccgattgtac ttgagtccat
      901 gtaaattaca agggaatcct catctggcaa atgcctgggt gtccacccaa atacctgtct
      961 tttaagatcc aatctcatgt aggttaaaag tcccaactta tccacggcaa attctggctg
     1021 gtggatattg tagtataggt tataggggga attgacagac ctgctcccaa tgttgacaag
     1081 gtggaggcca agaatatgag aagcaactcc cgctgcaaga attataagcc tggaccaaaa
     1141 atctgtagtg aagctgaaca gccccaaact tgataggagt ataaatactg gcaggggtaa
     1201 aaaaatggcc agaaccttag acagattatc taggattgta cttatggcaa ttcctgcaaa
     1261 ctccatcaca tctcctgcat ttccggctga atagactatg tagaaggttc taaagatata
     1321 aaaatagacc agttgggatg caaaaaggat gcatgtaatg gatatgaaag caagtgaaat
     1381 ccagaaatta aatcctctgg aaagacttgt tgaaagaagg aaaaagagag aagcaaaggg
     1441 aatgtaaaaa acaacgggta gtctcatccc aacaggaaat tgaccatcct tgaatttttg
     1501 tctcaaagcg gcctcaagat atatgattgc agacagatag aatatgaaaa gaagataatc
     1561 ttccatggta cacccctaat tattagtctt aacattagta taccacccgg gtgaatactt
     1621 gaataacctt tgggaaaatt tctttatagg gtatatagac tatgtatgat ggatggtcga
     1681 taggataagg aggtaatgat tatggcaaag caaacaatta tggtaaatga gctgaccaat
     1741 ggtgttcttg acccgaagga gcccatgctt ggtcctgtaa gggatggagg taccattatt
     1801 gcaaacacag ctcctggatg ctggggaccc atgataacac cagcattaag gggtggtcac
     1861 gaggttacaa agccggtatt tgtggaaggt gctcaggtgg gggatgccat tgcaatctac
     1921 attaaatcag ttgcagtaac atcatcggtt gtagcatcgg gaaatgagtt caccatacct
     1981 gaaaattttg atggagaccc atttgtggct ggtaagtgtc cacattgtgg tgcaaagcat
     2041 ccagatacag tcctggaagg gataggtgat gcctcggtga gatgtgcaca ttgtggagct
     2101 gaaattgcac cctttaaatt cacccatggc tatacaatgg ccttcaatga ggagaagtca
     2161 gtggggctta ccctgacaaa ggaaaaggcc gaagaaatgg caaagaaggc taaggaggtc
     2221 atggcaattc cggatgcctc tatccagaac ccaattgtaa cctttgctcc ccatgatata
     2281 accggcaaca tatcaagaat gagaccattc ataggtcagc ttgggacaac gccttcagtg
     2341 gcaatgccag actcacataa tgcaggtgat tttggtgcat tcctgatagg ggcaccccat
     2401 gagtatgcaa tagacaatga aaccctccaa aggagcaaga ctgacggaca tatggacatc
     2461 aacagggtga gacaaggagc tgttgttatt tgtcctgtca aggttgaagg cggaggagta
     2521 tatgtaggag atgtccatgg tatgcagggc gatggtgaga ttgcaggaca caccactgat
     2581 gtatccgcaa ttgtaaccct aaaggtcaaa ctgttgaagg gacttaagct cgacggtcca
     2641 ataatactac ccctggagga ggacctgcca caccttgcaa ggccaatcac tgatgctgaa
     2701 aagaccaagg caattaaact tggaagtagc cttggaatgg atgaatacga agagacagcc
     2761 cccatttcat ttgtgggtac cggtgaaaat cttaatgttg ccattgataa tggcctggaa
     2821 agggctgcag cagtattggg aataactgtt cctgaggtta agaacaggac cacaatcaat
     2881 ggtggtctgg aaatcggaag gtatcctggg actgcaacag taacattttt ggctcctgtt
     2941 gaccttttgg aaaaagcagg acttttggaa atggtaaaag accagtataa tatcaaataa
     3001 gaggataaac cggataggcc tggtgcctat ccgtgtgaat gattgtggag gtttataatg
     3061 agacttttca tttgcataga ccttggaaag gaccagctgg atattgttga aatgttaaag
     3121 aaaagactgg agggcgaatc aattaaggga agatttgcaa agcctgagca tatgcacttg
     3181 acccttaact tcattggtga gattgaagat gaaaaattaa gggatgtaat tgaggtaatg
     3241 gatagggtat actttagtgg gtttgacatt cacatgtctg gactggggtg gttccacaag
     3301 agatctggaa agatttactg ggttggtctg gaaagaagtg aggagctgtt tggccttcag
     3361 gaagacctgc acaaggccct attggaccgg ggttttaatc ttgaaaaaag aggctacaca
     3421 ccacacataa ccttgggaag ggctgtaagg gtagtggagg ggtttgaccc ttcaaggtat
     3481 acggagttgg ttcagggaac acagatcatt gtagataaga tagtattgaa gaagtctgag
     3541 aacacaggca gtggtgttat tcacacaata ctgtattcaa aggcggcaaa ggacagtttg
     3601 agatccaagg aggagaggaa ttgatcaggg taaatttaac gg
//
LOCUS       DASRQF010000055         2201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_707661, whole genome shotgun sequence.
ACCESSION   DASRQF010000055 DASRQF010000000
VERSION     DASRQF010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707661"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2201)
                     /locus_tag="VFD79_01440"
     CDS             complement(<1..>2201)
                     /locus_tag="VFD79_01440"
                     /inference="COORDINATES: protein motif:HMM:NF015380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HZH92739.1"
                     /translation="SNSQQSGQNYASTGGRRPSGGIIKYIIIFVVLMVLSSLISRFLG
                     GGSDAPISTPGPGVSNGGYVQETEGPSYDSGAYSVDRTVSDLARPKRTTLIGGGRDRA
                     TIMVYLLGTDLESRSGMATMDLQEMLEADLSENVNIVIETGGTAQWKNDVMSNRTNQR
                     YLISSQGLSRLEDNLGRKSMVDPDTLTDFIRYSQQNFPAERYFLIFWDHGGGSVTGYG
                     YDEFVKGETMTLDEIERALFNSGVEFDMVGFDACLMATLETAFVVEPYSDYLIASEEL
                     EPGIGWYYTGWLNELSRNPSIETIDLGKKLIDDYVSEVKRRVPKSQATLSLVDLAELS
                     ATVPPAFSAFSQSTAELIDTEDYKRVSDSRAGTKEFASSSKINQIDLIHFANRLGTDE
                     AVGFAKALEGAVKYNRMSDNITNANGVSIFFPYGKLSDVSSMLQTYEEIGMDENYSRA
                     IKSFANVNAGGQMASPGGGLLETLLGGGQSGGSQGIGTDLIGSLIGNFISQGNFSSIT
                     GGTQQAPDWIDEDQITQSTEYYSGNMLDPASIKITVKDGQRVLELTEEEWDLVHNMEL
                     SVFIDDGEGFIDLGRDNVFEYNEDGDLILEFDGTWMAINDRIVSYFMTSYDYRGDDYK
                     ILGRVPAMLNNQLVDIIISFDQDNPYGEILGARIVYDREEETPNLPKGLIDIEPGDSI
                     DYLTDYYTYGGDFDDTYYLGDPYRATGDWYIENLAITNLDYMMTYKITDIYG"
BASE COUNT          526 a          564 c          443 g          668 t
ORIGIN      
        1 ccatatatat ctgtaatctt ataggtcatc atatagtcca ggtttgtaat ggcaagattc
       61 tctatatacc agtctcctgt tgccctatat gggtccccaa gatagtaggt atcatcaaag
      121 tctccaccat aggtgtagta gtcagtgaga tagtcaatac tgtccccggg ttcaatatca
      181 atcagaccct ttggaagatt gggcgtttct tcttccctgt catagacaat ccttgcacca
      241 agtatctccc catatgggtt gtcctggtca aaggatatga ttatatccac aagctgattg
      301 ttaagcatgg caggaaccct tcccaaaatc ttgtaatcat ctcccctgta atcatagctg
      361 gtcataaagt agctaacaat cctgtcgttt atggccatcc aggttccgtc aaactcaagt
      421 atcaggtctc cgtcttcatt gtactcgaat acattgtccc tgcccaggtc tatgaagccc
      481 tctccatcat cgataaatac gctaagctcc atattgtgga ccaggtccca ttcctcttca
      541 gtcagttcca ggaccctctg tccatccttg acagtgatct ttatggatgc tggatcaagc
      601 atgtttccac tgtagtattc ggtggactgg gttatctggt cttcgtcaat ccagtcaggc
      661 gcctgctggg tccctccggt tattgatgaa aagttacctt gtgatatgaa gtttccaatg
      721 agggagccta tcaaatcagt ccctattccc tgacttccac ctgattgtcc tcccccgaga
      781 agtgtctcca gaagtcctcc tcccggagat gccatctgtc cacctgcatt tacatttgca
      841 aagctcttta tggcccttga atagttttca tccattccta tttcttcgta ggtctgaagc
      901 attgaagaaa catcgctcag cttgccataa gggaagaata tggatactcc gttggcattt
      961 gtgatgttgt cactcattct gttgtatttt accgctcctt ccagtgcctt agcaaagcct
     1021 acagcttcat ctgttcccag cctgttggcg aaatgtatta ggtcaatctg attgatcttc
     1081 gatgatgatg caaactcctt tgtacctgcc cttgaatctg ataccctctt gtagtcttca
     1141 gtgtctatga gctcagcagt tgactgggaa aacgcactga aggctggagg tacagttgca
     1201 ctaagctccg ccaggtcaac cagggaaagg gtcgcctgtg actttggaac ccttcttttg
     1261 acctcactta cgtaatcatc tataagcttc tttcctagat caattgtctc aatggatgga
     1321 tttcttgaaa gctcgtttag ccatcctgtg tagtaccaac ctataccagg ctcaagctcc
     1381 tcagatgcta tgaggtagtc tgaatatggt tcaactacaa atgctgtttc cagggttgcc
     1441 ataaggcatg catcaaagcc caccatgtca aattcaacac cggagttgaa aagggccctc
     1501 tcaatctcat ccagggtcat tgtttctccc tttacgaact cgtcatagcc atatccggtt
     1561 acggatcctc caccatggtc ccagaatatg aggaagtacc tttcagcagg gaaattctgc
     1621 tgggagtatc ttatgaagtc agttagggtg tcaggatcaa ccattgattt tcttccaagg
     1681 ttatcctcca acctgcttaa gccctgtgaa ctaatcagat acctctggtt tgtcctgttt
     1741 gacattacat cgttcttcca ttgggccgtt ccgcctgttt ctattacaat gttaacattt
     1801 tccgataagt ccgcctccag catttcctga aggtccattg ttgccattcc gcttctggac
     1861 tcaagatccg tacctagaag atacaccata attgttgccc tgtccctgcc tcctccaata
     1921 agggtggtcc ttttaggcct tgccaggtct gaaacagtcc tgtccactga ataggctcca
     1981 ctgtcatagc ttggtccctc agtctcctga acatagcctc catttgaaac tcccggtcca
     2041 ggagttgata ttggagcatc cgaccctcca cccaggaatc ttgatataag ggaggaaagg
     2101 accataagta caacaaaaat gataatgtat ttgataattc ctccagatgg tcttcttcca
     2161 cctgtggagg cgtagttctg tcccgattgc tgagagtttg a
//
LOCUS       DASRQF010000056         3959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_957309, whole genome shotgun sequence.
ACCESSION   DASRQF010000056 DASRQF010000000
VERSION     DASRQF010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957309"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VFD79_01445"
     CDS             <1..668
                     /locus_tag="VFD79_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HZH92740.1"
                     /translation="VNREDTPLRKDGEPDFESIDAIDLDLFNSDLVKLLEGESVQLPK
                     FNFITGKREASGKWISVDRDHPIIIEGIHGLNPKLTIDIPEKNKFKVYISALTQLNID
                     NHNRISTTDTRLIRRMVRDVKFRGNEPSRTFEMWQGVRSGEEKFIFPFQEEADAMFNS
                     SLVYEMSVLKKYIMPLLQEVTPESVYYSEAKKLLKFLKYFNPIEDESCIPPNSILREF
                     IGS"
     gene            680..1897
                     /gene="nagZ"
                     /locus_tag="VFD79_01450"
     CDS             680..1897
                     /gene="nagZ"
                     /locus_tag="VFD79_01450"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HZH92741.1"
                     /translation="MKKIICILLLTILLSGCGVTVQNDPPGEIPAENPSTQEPDPPSR
                     DEVLAQFVEDMTLQEKIGQLIMAGFEGTQVSEREVSLIEDYHIGGFIFFGRNITNEEE
                     TRNLIHELKTIKSDSPVQLFIGVDEEGGPVSRLSGIYRNLPPQSSLGESGDPEIAYEY
                     GSIQGEKLRRLGFNVNFSPVLDVDSNPNNPVIGNRAISNDPSLVAQLGIQVWKGIGDQ
                     GVVPVGKHFPGHGDTDVDSHTLLPVIEKSREELDETELVPFQASVDQGIPALMVGHLL
                     IPALDQEPASLSEGIMEDLLRDEMGFRGVLFSDDLTMGAITDRMSVSEAAVDFIGAGG
                     DVALICHGETEVMEAFKAIEAAVRDGIISEEYIDQKLVRIIRLKEDFKLGDEPVNDNS
                     QEGIDSRIEGLFD"
     gene            1913..2821
                     /locus_tag="VFD79_01455"
     CDS             1913..2821
                     /locus_tag="VFD79_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZH92742.1"
                     /translation="MKKVIGIDLGGTKINGAVVDESGEILRLKTMSTSKSGSPQEVIA
                     GIKGLVKILKEGEEISAIGIGSPGFIDSSNGKVLAVGGNIQGWAGTDIRGPIEREFGI
                     PVQVENDANVAGLCEGWIGAGRDLESFVMLTVGTGLGGGLYTKKEGLWRGSHFQGGEV
                     GHAILYPGGHQCNCGQRGCTEQYVSGTAVEDEYERLSGIRKTGREIFGSYHDDPISEK
                     VVRKFAWDFSNFLITIKNIFDPEAVIIGGGVILSRDTWWDEMIGNFNSQVNNNPGMEI
                     IPARHLDASGTIGAARAAMDRFVWEV"
     gene            2824..>3959
                     /locus_tag="VFD79_01460"
     CDS             2824..>3959
                     /locus_tag="VFD79_01460"
                     /inference="COORDINATES: protein motif:HMM:NF045064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92743.1"
                     /translation="MGKGRVYSGDYGNKRSERLIDEYMGILSGGKGKTTFYILPNGEL
                     LRSLRRRVVHKTGGAFHIGIMTFDDIVDRVVSGERLNVIQRPIQLNIVKMAIRKLADL
                     GRLEVFHTMADYDGFVDCVQEVIMEAKRALISPERFTEISRGDTYLQEVSLIYDFYQG
                     FLDENELVDREGSYMVALEVLRSGRTNFEGLERVMVDQFYDFRPIEIEILKQVHRMDL
                     EIILNLPFGGGEDTLLVGDTLRTLEDLGFEIIEDYNPGMNPMEIFAKHFVDGRETVDP
                     EGRITIIKGESPNLEIKRSITRIKKLLMEGVDPNSIHVIATTPFYRDLFLQLAASEDL
                     NIKAPIKDSLWDSRLIRGFIDILRLRESGGSREDIINLIKSPIM"
BASE COUNT         1147 a          739 c         1104 g          969 t
ORIGIN      
        1 ttgtaaacag ggaggataca cccctaagga aggatgggga gcccgacttt gaatcaattg
       61 atgccattga tctggacctc tttaacagtg acctggtcaa gctcttggaa ggggaatccg
      121 tacagcttcc aaagttcaac ttcataaccg gtaaaaggga agcctcagga aaatggataa
      181 gtgtggacag ggaccacccc ataatcatag aggggattca cggccttaat cccaagctga
      241 caattgacat acctgaaaag aacaagttca aggtgtacat atccgccttg acccagttga
      301 atatagacaa tcacaacaga atttcaacaa ccgacacaag acttatccga aggatggtca
      361 gggatgtgaa gttcaggggc aatgagcctt caaggacctt tgaaatgtgg cagggagtaa
      421 ggtctggtga ggagaaattc attttcccat tccaggagga ggcagatgcc atgttcaatt
      481 cctcactggt ttatgaaatg tctgtactga aaaaatatat catgcctctt ttgcaggaag
      541 ttacaccgga aagcgtctat tactcagagg caaaaaaact ccttaagttt ctcaagtatt
      601 tcaacccaat agaggatgaa agctgcatcc cgcccaattc aatactcagg gaattcatag
      661 gaagctgatt ggagggcata tgaagaagat aatatgtatt ttgttgctga caattctact
      721 ttcaggttgt ggagttacag tccagaatga ccctcctgga gaaattcctg ctgagaatcc
      781 gtccacccag gaaccagacc caccatcaag ggatgaggtc ttggcccagt ttgtggagga
      841 tatgaccctt caggagaaaa tcggtcagct gattatggct ggatttgaag gtacacaggt
      901 atctgaacgg gaggtttccc tgattgagga ttatcatatt ggtggtttca tattttttgg
      961 aagaaatatt acaaatgagg aggagaccag gaatcttatc catgaactta agacaatcaa
     1021 gtcagacagt ccagttcaac tgtttattgg cgtagatgag gagggtggac ctgtaagcag
     1081 gctttcagga atctatagaa accttcctcc acagtcaagc ctaggtgaga gtggagaccc
     1141 ggaaattgca tatgagtacg gatcaatcca gggtgaaaag ctcagacggc tgggctttaa
     1201 tgtaaacttc agtccggttc ttgatgtgga ttcaaacccc aataatccag tgattggaaa
     1261 cagggcaatt tccaatgatc caagccttgt tgcccagttg ggaattcagg tttggaaggg
     1321 cataggtgac cagggggtgg tcccagttgg aaagcacttc ccaggccatg gtgatacgga
     1381 tgtggattcc cacacccttc ttccagtgat agaaaaatcg agggaggaat tggatgaaac
     1441 tgagctggtc cctttccagg cctcggttga tcagggaata ccagccttga tggttgggca
     1501 ccttttgatt cctgcccttg accaggagcc tgcatcctta tcggagggga taatggagga
     1561 ccttcttagg gatgagatgg gctttagggg agtccttttc tctgatgatc tgaccatggg
     1621 agccataaca gacaggatgt cagtaagtga ggcagcagtt gactttatag gggcaggggg
     1681 agatgttgcc ctgatctgcc atggtgagac ggaagtaatg gaggccttta aggcaataga
     1741 ggcagcagtc agggatggaa tcatttcaga ggagtatatt gaccagaagc tggtgaggat
     1801 aataagatta aaggaagact tcaaattagg ggatgaacct gtcaatgaca atagccagga
     1861 aggcattgac agcaggatag aaggactatt tgattaggga gtgatgcctt gtatgaaaaa
     1921 ggtaattgga attgatcttg gtggaacaaa gataaatggt gctgtagtgg atgaatccgg
     1981 tgagatatta agattgaaga ccatgtccac aagcaagtca ggctctcccc aggaggtaat
     2041 tgctggaata aagggattgg tcaagatcct aaaggagggg gaggaaatat cagccattgg
     2101 catagggtca ccaggcttta tagattcctc caatggaaag gtgctggcgg ttggtggaaa
     2161 catccagggc tgggccggaa cggacataag ggggcccatt gaaagggagt ttgggattcc
     2221 ggttcaggtt gagaatgacg ccaatgttgc aggcctatgc gaaggatgga ttggtgcagg
     2281 cagggatttg gagagctttg tcatgctgac ggtgggaact ggactgggag gtggcctgta
     2341 tacaaaaaaa gaaggccttt ggcgaggcag ccacttccaa ggtggggagg ttggacacgc
     2401 aatactttac ccaggaggcc accagtgtaa ctgtggccag agggggtgca ctgaacagta
     2461 tgtttccgga acagcagttg aggatgaata tgaaaggctt tctggtataa gaaaaaccgg
     2521 aagggagatc ttcggatcct accatgatga ccccatttct gaaaaagtgg taaggaaatt
     2581 tgcctgggat ttttccaact tcctcataac aatcaagaac atttttgatc cggaggcagt
     2641 gataatcggc gggggagtga ttttatccag ggacacctgg tgggatgaga tgatcggcaa
     2701 cttcaacagc caggtgaaca acaatcctgg tatggaaatc atacctgcca ggcatctgga
     2761 tgcatcagga accatagggg cggccagggc ggccatggat agatttgtat gggaggtgta
     2821 gtcttgggaa agggaagggt ctactccgga gattacggaa acaagagaag tgagcgtctg
     2881 atagatgaat acatggggat actgtctgga gggaagggta agactacctt ctatatcctt
     2941 cccaacggtg aacttctcag gtccttgaga aggagggtgg tccacaagac aggtggagcc
     3001 ttccacattg gtataatgac ctttgacgat attgtcgaca gggtcgtttc aggggaaagg
     3061 ctcaatgtca ttcagcggcc aattcagctt aatattgtta aaatggccat aaggaaacta
     3121 gctgatttgg gaaggctgga ggtcttccat acaatggcgg attacgacgg ttttgtggac
     3181 tgcgtgcagg aagtcataat ggaggctaag agagctctga tctcccctga gagattcact
     3241 gagatttcaa ggggagacac ctatctccag gaggtttcct taatctatga tttctatcag
     3301 ggctttctag atgagaatga acttgtggac agggaaggta gctacatggt cgcccttgag
     3361 gttttaaggt ctggaaggac taattttgaa gggcttgaaa gggttatggt tgaccagttc
     3421 tatgatttca gacccattga gattgagata ctcaagcagg tccaccgcat ggatcttgaa
     3481 ataattctaa accttccatt tggagggggt gaggataccc tgttggtggg ggatacctta
     3541 agaacacttg aggaccttgg gtttgagatt atcgaggact ataatccagg aatgaatccc
     3601 atggagatat ttgccaagca ttttgttgat ggaagggaga cagtggaccc agagggtaga
     3661 attacaataa taaagggtga aagtccgaac cttgagatca agagatccat aaccaggata
     3721 aagaagcttt taatggaggg agtagatcca aattccatac atgtcatagc gaccactccc
     3781 ttttacaggg acttgtttct tcaactggca gctagcgaag acctcaatat caaggcccct
     3841 atcaaagatt ctctgtggga tagcagactt atcaggggct ttattgatat tctaaggcta
     3901 agggagtcag gtggaagcag agaggacatt atcaatctga tcaagtctcc cataatgaa
//
LOCUS       DASRQF010000057         6731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_707025, whole genome shotgun sequence.
ACCESSION   DASRQF010000057 DASRQF010000000
VERSION     DASRQF010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6731
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707025"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..1007
                     /locus_tag="VFD79_01465"
     CDS             162..1007
                     /locus_tag="VFD79_01465"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HZH92744.1"
                     /translation="MRVVNVSEITDVVEKLCIESNYYLPEDVRIALENALEVEESPLG
                     KEILQDILKNQEIARTKNVPICQDTGLAVLFLELGQDVRLIGGALEEAINEGVRRGYE
                     KGYLRKSTVDDPFMERKNLGDNTPAIIHTKIVPGDKVKMILAPKGGGSENMSALAMLK
                     PSDGVEGIRKFVLDTVEKAGSNPCPPIIVGVGIGGTIEKTTLIAKQSLLRNIGEHNPN
                     PKIAELEKELLVQVNNLGIGPQGFGGRTTALAVHIETFPAHIASMPVAINIQCHAARH
                     LEATI"
     gene            1024..1572
                     /locus_tag="VFD79_01470"
     CDS             1024..1572
                     /locus_tag="VFD79_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HZH92745.1"
                     /translation="MPKRVNTPLTDEVVKDLKAGDRVLLNGIIYTGRDAAHKRLVDMI
                     DRGENLPMELKDQTIYYVGPCPAKPGQAIGSAGPTTSGRMDAYTPKLMDHGIRGMIGK
                     GLRNQAVVDSIIKHTGVYFAAIGGAGALLAEAIKEAEVIAFPDLGAEAIYRLKVEEFP
                     VTVIIDSQGNDLYKIGKEQYKK"
     gene            1720..2262
                     /gene="pgsA"
                     /locus_tag="VFD79_01475"
     CDS             1720..2262
                     /gene="pgsA"
                     /locus_tag="VFD79_01475"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HZH92746.1"
                     /translation="MTLLNIITIFRIILIPIYLFIFFSEVENKVFILGMIFMIAGASD
                     VLDGYIARRFNLESKLGAALDPFADKMMSFTVLITFTMIGLIPIWILIPMVVKEIVMI
                     GGGLILYTKHGKAVIPSNKYGKVATFSLYAAVLSIILNLNMSLSVILLSITVILNLVA
                     FYNYLKIFKKLLNDEKAGRV"
     misc_binding    2314..2524
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2569..5214
                     /locus_tag="VFD79_01480"
     CDS             2569..5214
                     /locus_tag="VFD79_01480"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005956266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HZH92747.1"
                     /translation="MRENLPKNYDPKEFEDRLYGFWNENGYFTPEIDQNKKPYTIMMP
                     PPNVTGSLHMGHALNNTIQDILIRWKRMEGYSALWLPGTDHASISTEAKVVEKIKSEG
                     RSKDELGREGFLKEAWEWTDIYGGTIKNQLKKLGVSCDWTRDRFTLDEMLSNAVEEVF
                     VRLYDKGLVYRGDRIINWCPSCHTAISDAEVDHEDSHGHIWHVSYPIKGSDETITIAT
                     TRPETILGDLAVAVHPEDERYAHLVGKTVILPLVGREIPIIADSYVEMEFGTGALKIT
                     PSHDPNDFEVGARHGLGQCVVIDEEGYINENGGEFQGLERFAARKAIAEKLEKEGFLK
                     EVKDHENAVGHCERCKTVIEPIISKQWFVKMEPLARPSLEAYYNGEINFVPERFGKVY
                     VHWLENIKDWCISRQLWWGHRLPVYYCDDCGEVIVSRDDVKTCNKCGSTNIRQETDTL
                     DTWFSSALWPFSTLGWPEETEDLKYFFPTDVLVTGYDIIFFWVIRMIFSSLEQMGELP
                     FKDVFLTGLVRDSEGRKMSKSLGNGIDPLEIIDEYGADALRFTLITGNTPGNDMRFII
                     DKVEANRNFANKLWNATRFVLMNLDDDMEKATLEGMELQVEDKWILTRLNTVIGEIKE
                     NMDKYEIGLAGQRIYDFIWEDYCDWYIEMVKSRLYGEDEKSAETAKNVLVVALRETLK
                     LLHPFMPFITEEIWQHLPGESEALIVESWPTPDTNMIYNKEKEDIEFIKTAIKGIRNA
                     RAEMNIVPSRKSKLIFVLEDDSLKSVIEDSKVKFMTLASAEEIVFEKDKKTLGEDNIS
                     VVLDGCEVYLPLSDLIDYEKEIERLQKEKSKLEDEINRVKSKLSNEGFVAKAPAKIVE
                     EEKEKQKKYSQMLENVMERLESLKK"
     gene            5227..6528
                     /locus_tag="VFD79_01485"
     CDS             5227..6528
                     /locus_tag="VFD79_01485"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HZH92748.1"
                     /translation="MNYEEALAYINDKEKYGSRLGLDSIRTLLGHLGDPHKGLQYIHV
                     GGTNGKGSTSAYLSQCLKEAGYRAGMYTSPYLERFTERIQINGEDIPQDVLGEITGRV
                     KKAADRMVQDGMEHPTTFELVTAVGFVYFKNSNVDFVILEVGLGGRFDSTNVIQSSVA
                     SVITTIDYDHTDVLGNTLAEIAYQKAGIIKEKGLVVSYPQQMEAEKVIAEVAYDKGAD
                     FVKLPVENLKITSSTYQGSIFDFEHKGTKYENLKITMLGKYQVMNAALAVTTLLELKS
                     RGILDFTHEQLRSGLLKTKWKGRLEVVSESPIFLIDGAHNLQGISLLAEAVKMFPYRR
                     LILGMSVLKDKDYTHMIEKLVPLAAEVVVTEVNMPRRLEAELLGKEVQKFTPNVHVEK
                     TLEGAVRKSISLAHEDDMILFGGSLYMIGEVRTHAINLLTR"
     gene            complement(6521..>6731)
                     /locus_tag="VFD79_01490"
     CDS             complement(6521..>6731)
                     /locus_tag="VFD79_01490"
                     /inference="COORDINATES: protein motif:HMM:NF025635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HZH92749.1"
                     /translation="QTEANSYPRDNGEINVNLKLIELEETLFSMYISVPNQEQANILM
                     DKWEKSPDYYYKSIIELFIDNKPTI"
BASE COUNT         2202 a         1131 c         1658 g         1740 t
ORIGIN      
        1 gaaggtttac tctgagggag ccaacaaggc tgaggcaaaa gcaagaaaaa ccttaagaaa
       61 agtctataaa aaaatcggat tcataccaag aggataattg tccctccttt tagcattagg
      121 gcttatagga gagatagcta cagtaggaaa ggaagaatta tatgagagtg gtcaatgtat
      181 ctgaaataac agatgttgtt gaaaaacttt gcatagaatc aaattactat ttaccagaag
      241 atgtaagaat agctcttgaa aatgccctgg aagttgaaga atcacctttg ggaaaggaaa
      301 tactccagga catcttgaaa aaccaggaaa tagctaggac aaaaaatgtc cccatatgtc
      361 aggacactgg attggcagtc cttttccttg aattgggaca ggatgtgaga cttattggtg
      421 gagcattgga agaggctatc aatgaaggtg taagaagggg atatgaaaag ggatacctta
      481 gaaaatcaac agtggatgac ccatttatgg aaagaaagaa tctaggggac aacacacctg
      541 caataattca tacaaagata gtaccaggag acaaggtaaa gatgattttg gcccccaaag
      601 gtggtgggag tgagaacatg agtgcccttg ccatgctaaa gccatcggat ggagttgaag
      661 ggataaggaa attcgttctg gacacggtgg aaaaggcggg atcaaaccca tgtcccccta
      721 taatagtcgg cgtgggaatt ggtggaacca ttgaaaagac aaccttaata gcaaagcagt
      781 ccctccttcg gaatattgga gaacacaatc caaatcctaa gattgctgag cttgaaaaag
      841 agctgcttgt gcaggtaaac aacctgggta tagggccaca gggctttggt ggaagaacaa
      901 cagcgctggc ggtccacata gagacctttc cagctcacat agcaagtatg ccagtggcca
      961 ttaatatcca atgtcatgca gcaaggcacc tggaagcaac aatataaatt ggaggtagtt
     1021 gatatgccta aacgagtaaa tacacctttg accgacgaag tggtcaagga cttaaaggct
     1081 ggggataggg ttctccttaa cggtatcatc tacacaggaa gagatgctgc ccacaaaaga
     1141 ttggtggata tgatagatag aggggaaaac ctcccaatgg aactaaagga tcagacaatc
     1201 tactacgtag ggccctgccc ggcaaaacca ggtcaggcaa taggctctgc aggacccacc
     1261 acaagcggca ggatggatgc ctacactcca aaacttatgg accacggaat aaggggaatg
     1321 attggaaaag gcctgagaaa ccaggctgta gtggattcca tcataaagca cacaggagta
     1381 tatttcgctg caatcggagg agcaggagcg ctattggcgg aagccatcaa ggaggcagag
     1441 gtgatagctt ttccagacct tggtgcggag gcaatatata ggctgaaggt tgaggaattt
     1501 ccagttacag tgataataga cagccaagga aatgaccttt acaaaatagg gaaggaacaa
     1561 tacaaaaaat aggacggtgt aaacaatgtc ataaatcata gggcaacccc tcaattgtag
     1621 gtagcctgcc ttaaccttga tacggttgac cagaagtatt aaataaagta gaataactct
     1681 aaggcctgga aaaatggcat aagtataagg agagaaaata tgacactact taatattatt
     1741 accatattta gaattattct tatacctata tacttgttta tcttcttctc agaagtcgaa
     1801 aacaaggtat ttattctggg gatgatattt atgatagcag gtgcatctga tgttcttgat
     1861 ggatatatag caaggagatt caatcttgaa agcaaattgg gggcggcttt agaccctttc
     1921 gcagataaga tgatgagttt cactgtattg attactttca caatgatagg attaattcca
     1981 atttggatat tgatccccat ggtagtgaag gaaattgtga tgataggcgg aggccttatt
     2041 ctgtatacta agcacggaaa ggctgtaatt ccatcaaata aatatggaaa agttgcaaca
     2101 ttttccttat atgcagctgt tctttccatt atactcaacc ttaacatgtc actttcagtg
     2161 attttacttt cgattacagt catcttgaat ctcgttgctt tttacaatta tttgaaaata
     2221 ttcaagaagt tactcaatga tgagaaggct ggcagagttt aagagaatac agcattgaca
     2281 aacttcctct ttatagctat aattgtgact atatattttg tgatgagaaa gaggagtaat
     2341 ctgattctct taatagagaa aggattcatg gctgggagat cctaagcagg aatcagatga
     2401 aggtagcttt cgaacattca ggctgaacta gaagtaggct tgaacgggat accgttatct
     2461 atgaatgaag tgccatggtg atgccatgga attaaggtgg taccgcgaac ccttcgtcct
     2521 tatttttgga cggagggttt ttataatttt tgccagggag ggaaaaatat gagagagaat
     2581 ttaccgaaga attatgatcc aaaggaattt gaagatcgat tatatggatt ctggaatgaa
     2641 aatggttatt tcacaccaga aattgaccaa aacaagaagc catacaccat tatgatgccg
     2701 cccccaaatg taacgggaag tcttcatatg ggacacgccc taaacaacac catccaggat
     2761 atcctgatca ggtggaagcg aatggaaggg tattcagctt tgtggttgcc aggtactgac
     2821 catgccagca tatcaactga agctaaggta gttgaaaaaa tcaagtcaga gggcagaagc
     2881 aaggatgagt taggaaggga agggttcctg aaggaagcct gggaatggac ggacatctac
     2941 ggtggaacca ttaagaacca gcttaaaaag cttggggttt catgtgattg gacaagagac
     3001 aggtttaccc ttgatgaaat gttaagcaat gcagttgaag aggtttttgt taggttatac
     3061 gataaaggtt tggtatacag gggagatagg ataataaact ggtgtccaag ctgtcatact
     3121 gcaatctcag atgctgaagt agatcatgag gattcacatg gtcatatttg gcatgtaagc
     3181 tatcccatca agggaagtga tgaaactata accatagcca caacaaggcc tgaaaccatc
     3241 ctgggtgacc tggcagtagc tgttcatcca gaggatgaga gatacgccca tttggtaggt
     3301 aagacagtaa tactgccact ggtagggaga gagattccca taattgcaga cagctatgtt
     3361 gaaatggagt ttggaacagg tgccttgaag ataacaccat cccatgatcc caacgacttt
     3421 gaggtaggcg ctagacatgg tcttggtcaa tgtgtagtta ttgacgaaga gggatatatc
     3481 aatgaaaatg gtggagaatt tcagggtctt gaaagatttg cagcaagaaa ggccattgca
     3541 gaaaagttgg agaaggaagg cttccttaag gaggttaagg atcacgaaaa tgcagtaggt
     3601 cactgtgaaa ggtgtaaaac tgtcatcgag ccaataattt caaagcaatg gtttgtaaag
     3661 atggagccac ttgcaagacc atctcttgag gcatattaca atggcgaaat aaactttgtg
     3721 cctgaaagat ttggaaaggt gtacgtccat tggcttgaga atattaagga ttggtgtatt
     3781 tccagacagc tttggtgggg acacagattg cccgtttact attgcgatga ctgtggtgag
     3841 gtgattgttt caagagacga tgtcaagacc tgtaacaagt gcggcagcac caatatcaga
     3901 caggagacag atactctgga tacgtggttt tcatcagcac tttggccatt ttcaacccta
     3961 ggatggcctg aagaaactga agatttgaaa tacttcttcc ctacagacgt actggtaact
     4021 ggatacgata taattttctt ctgggttatc agaatgatat tttcaagtct tgaacagatg
     4081 ggagagcttc catttaagga tgtgttcctc acaggccttg taagggactc agagggaaga
     4141 aaaatgagca agtctcttgg taatggtatt gatccacttg aaatcatcga tgagtatgga
     4201 gcagatgccc ttagattcac cttgattact ggaaatactc caggtaatga tatgagattt
     4261 atcattgaca aggtggaggc caacagaaac tttgcaaaca aactttggaa tgcaactagg
     4321 tttgtattga tgaatcttga cgatgatatg gagaaggcaa cacttgaagg catggagcta
     4381 caggttgagg acaagtggat actgacaaga cttaacacag taataggtga gatcaaagaa
     4441 aacatggaca aatatgaaat tggccttgca ggtcaaagga tctatgattt catctgggaa
     4501 gactactgtg actggtatat tgagatggtt aaatcaagat tgtatggtga ggatgaaaaa
     4561 tctgctgaga ctgccaagaa tgtattggtt gttgcactaa gggaaacctt gaagcttctt
     4621 catccattta tgccattcat aactgaggaa atctggcaac accttccagg tgaaagtgaa
     4681 gcacttattg ttgaatcctg gccaacacct gatacgaaca tgatttacaa caaggaaaaa
     4741 gaggacattg agttcataaa aactgccata aagggaattc gtaatgcaag ggcggaaatg
     4801 aatattgtgc catcaagaaa atcaaagctg atatttgttc ttgaagatga ttcgttgaaa
     4861 tcagttattg aggattcaaa ggtaaaattc atgactcttg caagtgctga ggaaatagta
     4921 ttcgagaagg acaaaaaaac acttggtgaa gacaatatat cagtagtact ggatggttgt
     4981 gaggtttatc ttcccttaag tgacctcatt gattatgaga aggaaattga gaggctacaa
     5041 aaggagaaat ccaagcttga agatgagatc aatagagtca agtcaaaatt gtcaaatgaa
     5101 ggttttgtgg caaaagctcc tgcaaaaata gtagaggaag aaaaggaaaa gcagaaaaaa
     5161 tactcacaga tgctggagaa tgttatggaa cgactggaat cactaaagaa atagcaggag
     5221 gaacagatga actacgaaga ggctcttgcc tatataaatg acaaagagaa atacggttca
     5281 aggctaggcc ttgactccat aagaaccttg ttgggtcact tgggtgaccc ccacaaggga
     5341 ttacagtata ttcatgtggg ggggactaat ggaaagggct caacatccgc atatctgtca
     5401 caatgtttaa aggaagctgg ctacagggct ggaatgtata catcccctta cctcgaaagg
     5461 ttcactgaga ggatccagat taacggtgaa gacatcccgc aggatgttct gggggagata
     5521 acaggtaggg tcaagaaagc cgctgacagg atggtacagg acggcatgga gcatccaaca
     5581 acatttgagt tggtcactgc ggttgggttt gtgtatttta aaaacagcaa tgttgatttt
     5641 gtaatacttg aggttggact tggcggtaga ttcgacagta ccaatgtaat ccagtcatct
     5701 gtggcttcag taataactac aattgattat gatcatacag atgtgcttgg taataccctt
     5761 gctgaaatag cttatcagaa ggctgggatc ataaaagaaa agggccttgt ggtttcatat
     5821 cctcagcaaa tggaagcgga gaaggtgatt gcggaagtag cctatgataa gggagctgat
     5881 tttgtgaaac tccctgttga aaatctaaag attacgagca gcacctacca aggcagtatc
     5941 tttgatttcg aacacaaggg cactaagtat gaaaacttga aaattacgat gctgggcaaa
     6001 taccaggtaa tgaacgcagc attggctgtt acaacactcc tggaattgaa aagtagaggt
     6061 attttggact ttacacatga gcaattgaga agtggattat tgaagacaaa atggaagggt
     6121 cggcttgagg tcgtttcgga gagtccaata ttcttgatag atggtgccca caatcttcaa
     6181 gggatttcct tactggctga agcagtgaag atgttccctt acaggaggct aattctggga
     6241 atgagtgtac tgaaggacaa ggattacacc catatgattg agaagcttgt tccattggct
     6301 gcagaggttg tagtcactga agtaaacatg cctcgcaggc ttgaagcaga gctattgggg
     6361 aaagaggtcc agaaattcac acctaatgta cacgttgaaa agactttgga aggagccgtg
     6421 agaaaatcca tttctttggc tcatgaggac gatatgatcc tttttggagg atcgctttac
     6481 atgataggtg aagtgaggac tcatgcaata aacctactaa ctagatagta ggtttattgt
     6541 ctatgaaaag ttcaattatt gacttgtagt agtaatcggg agatttttcc cacttatcca
     6601 taaggatatt agcttgctcc tgatttggta cgcttatata catgctgaac agtgtttctt
     6661 ccaactcaat gagctttaga tttacattga tttccccatt gtctcttggg tatgagtttg
     6721 cttcagtttg t
//
LOCUS       DASRQF010000058         4527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1332354, whole genome shotgun sequence.
ACCESSION   DASRQF010000058 DASRQF010000000
VERSION     DASRQF010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332354"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VFD79_01495"
     CDS             complement(<1..804)
                     /locus_tag="VFD79_01495"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HZH92750.1"
                     /translation="MNKETSGVDLISNLTHEELEELEEEAHHIVEEVDRSSRIRTYRG
                     PWSRIITVLSVIWVVFQLYFTTIGTMEAISFRAIHASVLVLFAFLIYPAWKNENRDRR
                     SPPLIDYVLIGINLAVFSYFVLNYNRIAMTGGFVSDFENLIGLVAIALVFIAARRASG
                     GLVWLSSIFLIYNFLGRMIPGTLGHGGFSASRIIGHMFWGSQGIFGSGIGVSATYIFV
                     FILFGAFLNLSGFSKLVNNLSLSLVGRTAGGPAKVSVVVSGFLGMINGSA"
     gene            complement(932..1147)
                     /locus_tag="VFD79_01500"
     CDS             complement(932..1147)
                     /locus_tag="VFD79_01500"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF025903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HZH92751.1"
                     /translation="MQLNFSRRINMPILQVEMLKGRTEEQKRAMVEKVTDVLVETVNC
                     KRDAVRIIIREMEDENFAIGGVLKSDM"
     gene            complement(1149..2423)
                     /locus_tag="VFD79_01505"
     CDS             complement(1149..2423)
                     /locus_tag="VFD79_01505"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZH92752.1"
                     /translation="MKNHLFIEKNENGSIQIPKEVLEKEHQGRHQEYIFYEENGKLVL
                     APSIHSVQRLYIEPTAACNLNCVTCIRKTWKETMGFMKREEFDKLVEDIKEIKTIDSV
                     MFGGFGEPTHHPDIYYMIKRISDLGIRTEITTNGTNLTEENIRKLMDAGLKNLWVSID
                     SMDVARFNEIREGADFGQVFDNLMTLKRMNKGKNKKLTLGIAFVVTKDNVEDLSRLRS
                     FATKVWAEKISVSNVIPYDQEMEDKMLCKKTLFKPLAYRAQDVIRKGTKEVMIPEISL
                     PRIDYNKHTKEGLHDIHASMMKTKTLHEEFKEHNDYCRFINEGNVFVRWDGEVAPCMG
                     LIHSYTTFLNGNQRHMKEHTFGNLREKSLKDIWDQEDYRDFRQKVYDFEFSPCVLCGG
                     CEDVDSNDKDCLSNTFPACGGCLWAQGVIQCP"
     gene            complement(2452..3378)
                     /locus_tag="VFD79_01510"
     CDS             complement(2452..3378)
                     /locus_tag="VFD79_01510"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HZH92753.1"
                     /translation="MSYFIYTFFNIIFPLFITISAGYGIRSILPIDVRSLVRLQLYLL
                     LPIVLFVKVYESELSGEIVLSVSLATAFVMTAMYVMSFVIARLRKYNPEDTSVFVNAT
                     TFYNAGNFSLPLMQLLYINPIAVSIQAIIMLVHNIFFFTVGMFTIGSSRGNAKKALGY
                     VFRMPFLYVMITGIILRSINMTIWDPLWQSLSLMSTSYAGIALITLGAQLKETSFKIG
                     NLRIYLSNTLRLLVAPAIAFAVVMFMGLEGIIAQVLIIAMGAPTAVNVALTSMEFDNN
                     PEFASQAVLTSTVLSAVTLTMVIILVQLAIPI"
     gene            complement(3433..4455)
                     /locus_tag="VFD79_01515"
     CDS             complement(3433..4455)
                     /locus_tag="VFD79_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HZH92754.1"
                     /translation="MDYNEIRENAKEILAPLCLVCKECNGVVCRGWVPGVGAKGTGNS
                     FIRNYDYLQQVKLVMDMIYEGKGQDTSINMFDKDFTAPIFVAPIGGMDINYGGRIVEG
                     EYHRRTLVGAKNAGIAAFTGDGANPTYFEEPLEHLEAVEGWGVPTLKPWSQEMVFEKI
                     KTVEKIGALALAMDIDSAGLVHLAKSGKPVFTKTVEDLREIVEGTRLPFIVKGVMSAE
                     GARKAADSGAYGIVVSNHGGRVLDNTPATTEVLPEIREAVGDRMKIFVDGGIRTGADV
                     FKAIALGADAVLIGRTFTIMAIGGGEEGVQLYAEKLIAELKDTMLMTGCSTITDITRD
                     KIILPK"
BASE COUNT         1118 a         1139 c          895 g         1375 t
ORIGIN      
        1 tgcagacccg tttatcatcc caaggaatcc cgataccacc acggatacct tggcaggccc
       61 tcctgccgtt cttcccacaa gggaaaggga caggttgttt acaagcttgc tgaagccgct
      121 aagattaaga aatgccccga aaaggataaa gacaaaaata tatgttgctg aaaccccgat
      181 tccgctgccg aatatcccct gacttcccca aaacatgtga ccgattatcc tggaggctga
      241 gaatccacca tgacccaggg tcccaggaat catcctaccc aggaagttgt agatgaggaa
      301 tatggaagat agccacacaa gccctcccga ggcccttctt gcagctatga ataccagggc
      361 gatggcgaca agtccaatca ggttctcaaa atctgataca aagccccctg tcatggcgat
      421 cctgttgtag ttcagtacaa aatatgaaaa aactgcaagg tttattccta tcaggacata
      481 atcaatcagg ggaggactcc ttctgtccct gttttcattt ttccaggcag gatagatgag
      541 aaatgcaaac aagaccaata cagatgcatg tattgcccta aagcttattg cttccatggt
      601 tccaatggtt gtgaagtata gctgaaaaac cacccatatg acagagagaa ctgtaattat
      661 tctggaccag ggacccctat aggttctgat cctgctgctt ctatccactt cttcaacaat
      721 gtggtgggct tcctcctcca actcctccag ctcttcatgg gtcaaatttg aaattaagtc
      781 tacaccgcta gtttccttgt tcatcactta cacctgctca cctgttatta caacctttat
      841 tctatttcac caaggggcat aaatcaattg aaatttaatt ggagctttaa aaagcaagac
      901 tactggggag cctccacagt agcctttttt cttacatgtc tgattttaga actccaccga
      961 ttgcgaagtt ttcatcctcc atctccctga tgatgattct tacagcatcc ctcttgcagt
     1021 tgacagtctc cacaagaaca tctgttacct tttcaaccat ggctctcttt tgttcctctg
     1081 ttcttccctt aagcatttcc acctgaagta ttggcatatt aatcctcctt gaaaaattta
     1141 gttgtattct aggggcactg gattactccc tgcgcccaga gacatcctcc gcatgccggg
     1201 aatgtattgg ataggcagtc cttgtcattg ctatccacat cctcacatcc tccgcaaagg
     1261 acgcagggtg agaattcaaa gtcgtagact ttctgcctga agtccctgta atcctcctgg
     1321 tcccaaatat cttttaatga tttttcccta agatttccga atgtgtgctc cttcatatgt
     1381 cgttgattgc cattaagaaa ggtggtgtag gaatggataa gtcccatgca tggggctacc
     1441 tccccgtccc atctcacaaa cacgttcccc tcattgatga agcgacagta gtcattgtgc
     1501 tccttgaact cctcgtggag ggtttttgtc ttcatcatgg atgcgtgtat gtcatggagt
     1561 ccttcctttg tgtgtttatt gtaatcaatt cttggcaggc tgatttccgg tatcataacc
     1621 tccttggttc ccttcctgat cacatcctgg gccctgtagg caaggggctt gaatagggtc
     1681 ttcttgcaaa gcatcttgtc ttccatttcc tggtcgtagg gaataacatt gctaactgag
     1741 atcttctcag cccagacctt ggttgcaaag ctccttagcc ttgacaggtc ctccacatta
     1801 tcctttgtca caacaaaggc tattccaagg gtcagcttct tgttctttcc cttgttcatc
     1861 cttttcaggg tcatcaggtt atcaaacacc tgaccaaagt cagcgccctc acggatctca
     1921 ttgaacctgg ccacatccat gctgtctata gaaacccaaa ggtttttcag gccagcatcc
     1981 ataagcttcc ttatgttttc ctcagttagg ttggtaccgt ttgttgtgat ctctgtcctg
     2041 attcccaggt cgctgatcct ttttatcatg taataaatgt ccgggtggtg tgtgggctca
     2101 ccaaagcctc cgaacatgac ggagtcaatg gtcttgattt cctttatgtc ctcaaccagc
     2161 ttgtcaaact cctcccgctt cataaagccc atggtctcct tccaggtctt tcttatgcat
     2221 gtaacgcaat ttagattaca ggctgctgtg ggttcaatgt atagcctttg gaccgagtgg
     2281 atggaagggg caagcacaag ctttccgttt tcctcgtaga atatgtactc ctggtgtctt
     2341 ccctggtgct ccttttccag cacttccttt ggtatctgga tggagccgtt ctcgttcttt
     2401 tcaataaata gatggttttt catttaatca cctttccaaa tgtactttcc tttagatggg
     2461 tatggccagc tggaccaata ttatcaccat tgtcaacgtg acagcactaa gtaccgttga
     2521 tgtcaagacc gcctgggatg caaattcagg attgttgtca aactccatgg atgtcagggc
     2581 aacattcact gctgtgggcg ctcccattgc aattatcagc acctgggcga ttatgccctc
     2641 aagtcccata aacatgacca ctgcaaatgc tattgctgga gcaacaagca atcttagggt
     2701 attagaaaga tatatcctca aattcccaat cttgaagctt gtctctttaa gttgggcacc
     2761 cagagtgata agtgcgattc ctgcatagct tgtactcata aggcttaggg attgccaaag
     2821 tggatcccaa attgtcatat tgatacttct taatattata cctgtaatca ttacataaag
     2881 aaagggcatc ctgaaaacat atcccaaggc ttttttcgca ttccccctgc tgcttcctat
     2941 tgtgaacatg cccactgtaa agaagaatat attatgaacc agcattatta tggcctggat
     3001 tgaaaccgca attgggttta tatataggag ttgcatcagc ggaagactga aattaccggc
     3061 attgtagaag gttgttgcat ttacgaatac gcttgtatcc tccggattat acttcctaag
     3121 ccttgcaata acgaatgaca tcacatacat tgccgtcatt acaaatgcgg ttgcaaggga
     3181 tacagataat acgatctcac cgcttaactc gctttcgtaa acctttacga aaagtacgat
     3241 tggaagtaga aggtagagct gaagccttac cagacttctt acgtcgatgg gcaggatgga
     3301 cctgattccg tagcctgcag atattgttat gaaaagtgga aatattatat tgaaaaatgt
     3361 atagataaaa tagctcataa agtctctcca ttatgtattt ggggcacaga agaactgcgc
     3421 cccattttgc ttttacttag gtagtattat cttgtccctt gttatatccg tgattgtgga
     3481 gcagcctgtc ataagcattg tgtccttcaa ttccgcaatg agcttttccg cataaagctg
     3541 gactccctct tcccctccac caattgccat tatggtaaag gttcttccta taaggacggc
     3601 atctgctcca agagctattg ccttgaacac atctgcacct gttcggattc caccatccac
     3661 aaatattttc atcctgtctc cgacagcctc cctgatctct ggaagtactt cagttgttgc
     3721 aggggtgttg tcgagaaccc ttcctccgtg gttggaaaca actattccat aggctcccga
     3781 gtctgcagct tttctggctc cctctgctga catgactccc ttcactataa agggtagcct
     3841 tgtaccctcc acaatttccc ttagatcctc aacggtcttt gtaaagacag gcttgcctga
     3901 tttggcaagg tgaacaagtc ctgccgagtc tatgtccatt gccagggcca gggctccaat
     3961 cttttcaacg gttttgatct tttcaaatac catttcctgt gaccagggct taagtgttgg
     4021 gactccccag ccttcaacag cctccaagtg ctccagtggt tcctcgaaat atgtaggatt
     4081 tgctccgtcc cctgtaaatg ctgctatgcc tgcatttttt gcaccgacca gggtccttct
     4141 gtggtattca ccctcgacaa tccttccgcc gtagtttatg tccataccac caattggggc
     4201 aacgaatatg ggtgcggtaa aatccttgtc aaacatgttg atgctggtat cctgaccctt
     4261 tccttcatag atcatatcca ttacaagctt tacctgctgt agataatcgt agtttctgat
     4321 aaagctgttg cctgttcctt tggccccaac tcctggaacc caacctctgc agacaactcc
     4381 attgcattcc ttgcatacaa gacaaagcgg tgccagaatc tccttggcat tttccctgat
     4441 ttcattatag tccatggtca tcctccttta gcttttgatc tggttctctt ttatgtatat
     4501 tcctataatt ctcctgattc ttataat
//
LOCUS       DASRQF010000059         2053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_706302, whole genome shotgun sequence.
ACCESSION   DASRQF010000059 DASRQF010000000
VERSION     DASRQF010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706302"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VFD79_01520"
     CDS             <1..684
                     /locus_tag="VFD79_01520"
                     /inference="COORDINATES: protein motif:HMM:NF015820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaA family protein"
                     /protein_id="HZH92755.1"
                     /translation="NNDTGGGDILWPEKTTRLFQILREYSEDELGKLMNIKGDLLQET
                     YELYSSWTTGNDKIRAIDLYQGVAFEGINYNELNGVQKEYLNKSLLILSAMYGAASPD
                     TLIWSYRLDMTMKPLEETLYSYWKDEISQMFQNEDLVVNLASNEFARMVKVEKGKILN
                     IHFKKGLGKSSKRLSSYEGKKARGEMVGVLAQNKAYTREDIRNLAPQGFVFDQELSDR
                     DNYYYGLSI"
     gene            complement(681..1868)
                     /locus_tag="VFD79_01525"
     CDS             complement(681..1868)
                     /locus_tag="VFD79_01525"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZH92756.1"
                     /translation="MNRFLNFTYAGVQGFYWMYFAVIISFASVFLLDRGYSNSQIGFI
                     LAFANILAVLIQPVLADIADRSKKLSLISVVYVMGAALILGTGSLYFMNQRSIILTSV
                     FVITGALQISLQPLINSIAFHFTKAGSYINFGATRSVGSVAYALISSILGALVLAFGT
                     RSIPLTGVIVMLLLLSALLLTDKLFKRELTVHIPDSSFDIPNEDSAITLLQFMKRNKV
                     FVILTLGIMLVFFQNAVINNFLIQILRNIGGDSSQMGRLFSFMALLEMPGLFFFSRLR
                     KRFSSQTMLKLSSVAFIGIVFFTYLAPSVGFVYLAFLFQLVSFPLFLSSAVHLADEVM
                     EKGEAVKGQSLVTGMMTLSGVFASLLGGAILDIDGPSQLLLISTILAIVGTISIFFTV
                     NRV"
BASE COUNT          666 a          400 c          492 g          495 t
ORIGIN      
        1 aataatgaca ctggtggagg ggatatctta tggcctgaaa aaacaaccag gctctttcaa
       61 atcctaaggg aatatagcga ggatgagctg ggaaagctta tgaacataaa gggagatctt
      121 cttcaggaaa cctatgagct atacagcagc tggaccactg gtaatgataa gataagggca
      181 atagacctct accagggtgt agcatttgag gggataaatt ataatgaact caatggagtg
      241 cagaaggagt atctgaataa gagtctattg atcctttcag ctatgtacgg tgcagcaagc
      301 ccagataccc ttatctggtc ctataggctt gatatgacca tgaagcctct tgaggaaacc
      361 ctctacagct attggaagga tgagattagc cagatgttcc agaatgagga ccttgttgtg
      421 aatcttgcct ccaatgagtt tgcaaggatg gtcaaggtgg agaagggcaa aatcctaaac
      481 atccacttca agaaggggct aggcaagtct agtaaaaggc tctcaagcta tgaaggaaag
      541 aaggcaagag gagaaatggt tggggtgtta gctcaaaaca aggcctacac cagagaagac
      601 atcagaaacc tggctccaca aggctttgtc tttgaccagg agctttcaga cagggacaat
      661 tactattatg ggctatcgat ttagaccctg ttgaccgtaa agaaaatgga tattgttcca
      721 actatggcaa gaattgtgct tatgaggagc aactgggatg ggccgtcaat atcaagtatg
      781 gccccgccaa gaagacttgc aaatacacca cttaaggtca tcattcccgt aacaagtgac
      841 tgacccttga cagcctctcc cttttccatg acctcatcag ccagatgcac tgcagatgag
      901 aggaaaagtg ggaatgaaac aagctggaag agaaatgcaa gatacacaaa tcctacggag
      961 ggtgccaggt aggtgaaaaa aactatgcct ataaatgcaa ctgaggaaag cttaagcatt
     1021 gtctggctgg agaatctttt tctcagcctg ctgaaaaaga atagtccagg catctcaaga
     1081 agtgccataa aggagaacag cctccccatt tggctgctgt cccctccaat gtttctaagt
     1141 atctgaataa ggaagttgtt tattactgca ttctggaaga agaccagcat aattccaagg
     1201 gtgagaatca caaaaacctt gtttctcttc ataaactgaa gcagtgttat tgcagaatcc
     1261 tcattgggga tgtcaaagga tgaatccggt atatgtactg taagctccct tttaaaaagc
     1321 ttgtccgtca gaagaagggc tgaaaggagc agcagcatta caataacccc tgtcaagggt
     1381 attgaccttg tgccaaaggc caggaccaag gcacctagaa tggagcttat cagggcatag
     1441 gcaacggatc caacgcttct agtggctcca aagtttatat agcttcccgc cttggtaaag
     1501 tgaaaggcta tggaattgat taatggctgt agggatatct gaagagcgcc tgtaataaca
     1561 aatactgatg tgagtataat tgagcgctga ttcataaaat acagagaccc tgttccaagt
     1621 ataagggctg cgcccataac atacaccact gagatcaggg acagtttctt tgacctgtca
     1681 gcaatatcgg caaggacagg ctggataaga acagccagga tattggcaaa ggccaggata
     1741 aatccgatct gggagtttga atatcctcga tccagcaaaa acacggatgc aaagcttatt
     1801 atgactgcaa agtacatcca ataaaagccc tggactcctg catatgtaaa gtttagaaat
     1861 ctattcatag tatcaccatt tctttcgtta ttaatgtatt gtaacactca aaaagaaaac
     1921 taacaaccgg tattgaacgg tttatgaata ttgaaaggcg gggaggtaat cctatgggct
     1981 ttataagcga atggcaggat aattatgtgg tgaagcaggt gaaaaacctg aagctaccag
     2041 agtttggtga atc
//
LOCUS       DASRQF010000060        10797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1081453, whole genome shotgun sequence.
ACCESSION   DASRQF010000060 DASRQF010000000
VERSION     DASRQF010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 10797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10797
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081453"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VFD79_01530"
     CDS             complement(<1..725)
                     /locus_tag="VFD79_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HZH92757.1"
                     /translation="MKKRSVIFLDQLAYRFTENNVLGTGAQLTYFLILSLFPFLMVLL
                     NVISYTPLGREDVIMDLIKYMPTDIQQIIQTFHDDVVSSSSQGFLSIAAIAGLWTASS
                     GVKAVIKAINKAYNYAENRSFLRLKGLSLLFTVVLILMIIIVFVSLVAGRLIGSLVSD
                     FLGIEDLFLTMWNYLRFVIPLLFMILSFALLYKYSPSIKNRYSVPLRTTLPGAVFATI
                     GWIVLSLLFSFYVSNFGNYAVTYG"
     gene            complement(776..2764)
                     /locus_tag="VFD79_01535"
     CDS             complement(776..2764)
                     /locus_tag="VFD79_01535"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZH92758.1"
                     /translation="MENLKREDLTRFKFLSGTQISPDGRTCGFLLHMADEEENKYRSN
                     IYLWDEIDGTRQLTASDSDGSFVWKGKDRLLFSSIRDKADKEKKEKGYPLTCFYEIST
                     TGGEAQKAFQVYLDVSRFDHIKDNKYLLLGSFDRRLGEIQGLDDKEIEEKVKALKKEN
                     EYQVLDEIPFWTDGGDFTNKKRTRCYIFDSSTEELTPITDEFTNVESISIHPSKEKAI
                     LVTSSYTDKMSPYNHLYQLSLSSGTCEKISPISDFNYYYADYMDDRIIFVGTDMTSYG
                     INENPRIFVTEDEGLTYVRIAEPDLSLWSSVNSDCRYGMNRSFKVSGDHLYFLSTKEY
                     SCHLHRVSLNGNIEQVSLEDGSVDDFDVVDETIVFVGMRDLYLQDLYRLDHQEHRLTS
                     FNDWVVQDRKLSTPMVLTFNNEEISLTGWVLKPVDFDKNRRYPAILHIHGGPKTVFGG
                     VFHHEMQLWANQGYFVFYMNPRGSDGYGNEFSDIRGNYGNTDYEDIMLFTDKALETFP
                     SIDRERLGVTGGSYGGYMTNWIIGHTKRFKAAVSQRSISNWFSFFGTSDIGYYFATDQ
                     VGASPWKDYSALWDNSPLKYADQVQTPTLFIHSDKDYRCWIPEGLQMYTALKYHGVDS
                     KMVVFKNESHGLSRVGRPQGRIRRLKEIVDWFDRHLKD"
     gene            complement(2727..4475)
                     /locus_tag="VFD79_01540"
     CDS             complement(2727..4475)
                     /locus_tag="VFD79_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92759.1"
                     /translation="MHTKEYERLQLLESKATEKLIKYINKQEEIKYIYDRMLGLHPFL
                     QEESAKLPFNIWLCCDYRDKEGMTFGERLILEDDSLTPDEVEILREKSASYVSLFQIK
                     DFQEPYVVVTDVLTGEDHSLLEPQVSYVLKVGDYLLSRLGTVLGNSRMIGEVSFVPKS
                     VIHSFIRTLIRDFNKLDQDQRSEGTKTYLKNDALKIYDLFYQSAYKYFDPQGEDLIPV
                     YQELDEFEEYLSTKKSSSQIEKDLSNMIELYEYYLSEEGYSFKDMDKISIKDMLGDAI
                     YEKFITSGLVFNSYISTLKAYLSYRSKISSKYQGAYEEILQISRDRFHYLKEIIRRNS
                     LAQGDPLLSLQLKDHESDAIKSYLNDFDRFLLYVFDYPLELTEKKGHIKKVDQHNIID
                     FLEGDYPLYFSRPGQMKEPVVHFFFQMGLMLKLIKIRKNSLSVTANASEYLGMTREDK
                     YTLHLRGFWNQESIKGLVQLDSRNAKQMMKLLLQSVLRLKEGPTFSEDLAELDGLDSD
                     DLYLLVRYMTFMGILSNRGFDRLVSITPTGRMVATHLEEWSKPRKNIVINIGQYTKGN
                     KEEGYGESKAGGLDEV"
     gene            4668..6362
                     /locus_tag="VFD79_01545"
     CDS             4668..6362
                     /locus_tag="VFD79_01545"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HZH92760.1"
                     /translation="MKFGDYKYERPDLDRMEKDMDRLLENFRRADGPEDQSQVIRRIT
                     EIRNRFETMYTLVSIRHSIDTRDAFYEKEQEFMDENSPNYTSMVTRFYSELVGSPFKE
                     ELSREWGDQLFRLAHLQIKTFSQEIMDDLVVENKLSTRYDKLVAAAAIQFEGKTRNLS
                     QLRPFMESPDRDMRKRAYEAYTSYFQEKEEEIGEIYDQLVKVRDRMAKKLGYENFVQM
                     GYDRMSRSDYDHRMVRNFRDQVLETVVPVVSEMKERQRIRLGLDQLTYYDEPLGFLSG
                     NANPKGGPEWILEKGIRMYKELSEETGEFFDFMVERDLLDLLSKEGKRSGGYCTVIPD
                     YNAPFIFSNFNGTSGDIDVLTHEAGHAFQTYMSMDHELPEYKFPTLEACEIHSMSMEF
                     LTWPWMDGFFEEGVHKYKYSHLEGAVSFLPYGVTVDHFQHVVYENPNMTPKERKDVWR
                     DLERKYLPFKEYGENDFLESGGYWMRQGHIFSNPFYYIDYCLAQVCAFQFWRRSLEDR
                     EGAWNDYLDLCRAGGSMAFTELVHLANLKDPFKDGTVKYALEPVKDFLASIDDKEL"
     gene            complement(6385..7275)
                     /locus_tag="VFD79_01550"
     CDS             complement(6385..7275)
                     /locus_tag="VFD79_01550"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HZH92761.1"
                     /translation="MVLKKYRIGMRTIKTALAVSIGLFIAEVFNLQSPLFVGIGAIHT
                     MQATVSESFEAGKNRILGTVVGGLIALIFALYLPRNQIFMGIGIILVIHILNLVGWKK
                     AISLSAIVFVAVSLNHEYEMVPYAVSRILDTFVGIAVGVIVNYFVAAPKSYRMFDKYA
                     ERMMSGAKDYAYKLVVGKYKVDLKDFKSELAKIDKIFDLIQSETKIKLARNRDLPGSE
                     ITLSTIYDIYSDLSALQMMQHIAIISPDNIDLMEKLYSFTPIWEKKAIIGEKDIIFNY
                     HLQRLLKNIIYLLDNQNQED"
     gene            7578..8114
                     /gene="raiA"
                     /locus_tag="VFD79_01555"
     CDS             7578..8114
                     /gene="raiA"
                     /locus_tag="VFD79_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HZH92762.1"
                     /translation="MKMNYTGKNMEVTDALRDVTEKKLSKLEKYFQSDISGNVTFSTE
                     KNRKIIEVTINLPGTILRAEESSDDMYASIDKTVDVLERQVRKHKTKLQKRYRNNDTI
                     RFENVPSLTKEEEEDKPKLVRRKRFGLKPMSSEEAILQMELLRHNFFVFMDAESEEVS
                     VVYKRKDGNYGLIEPDFN"
     gene            8219..>10797
                     /gene="secA"
                     /locus_tag="VFD79_01560"
     CDS             8219..>10797
                     /gene="secA"
                     /locus_tag="VFD79_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HZH92763.1"
                     /translation="MGLFDKVFGTYSERELKKLDKTVEQILSLETEIQNLSDDALKNK
                     TNEFKDRFKNGEGLNEIMPEAFAVVREASWRVLGMKHFPVQIYGGIILHQGRIAEMKT
                     GEGKTLVATLPAYLNAITGLGVHVITVNDYLAKRDRQWMGKLFEFLGLTVGCVVHGMN
                     NKEKKEAYKADITYGTNNEFGFDYLRDNMVIYKEEMVQRKLYYAIVDEVDSILVDEAR
                     TPLIISGKGDKSTSLYGMADSFARTLSYRKLDPEETKSDPFNREIKEETVDYLIDEKA
                     KSIALTDKGTAKAESFFGIENLADVENMETAHHINQAIKARSIMKRDIDYVVQDGEVI
                     IVDEFTGRLMYGRRYSNGLHQAIEAKEGLDVRSESKTLATITFQNYFRMYEKLSGMTG
                     TAKTEEEEFREIYNMDVVEIPTNKPVVRIDNADSVYKTEAGKFKAVVDEIREKHQIGQ
                     PILVGTITIERSELLSKMLKKEGIRHEVLNAKQHEREAEIVAQAGRFGSVTIATNMAG
                     RGTDIVLGGNPDHMAKDEMKKKGYDNEVLAYVDGFFETDDQEVIEARKVYQELVKKHE
                     GETRVEHEKVVQAGGLHIIGTERHESRRIDNQLRGRSGRQGDPGSSRFYISLEDDLMR
                     LFAGDRIKGIIDGMNMPEDEPLEHALLTKTIEGAQKKVEGNNFSIRKHVLQYDDVMNK
                     QREIMYGERRKVLEGENLKDHIQEMVRGLVVDNVELYTRGEKYPEQWDFEGLETYLVK
                     QFGFRQGIFAGMDIESLTKEDLIDRIMKKSEEAYKVKEAEFGEDQFREIERVVLLQVV
                     DRKWMDHIDAMDQLRQGIGLRAYGQQDPVRAYQIEGFDMFEEMTRSIWEDTVGLLYHV
                     VKN"
BASE COUNT         3158 a         2259 c         2572 g         2808 t
ORIGIN      
        1 ccataggtca ccgcatagtt tccaaagttg gatacgtaga atgaaaacag aagtgaaagt
       61 acaatccacc caatggttgc aaacaccgca ccgggcaggg ttgtccttaa gggtaccgag
      121 tacctgttct ttatgcttgg actgtacttg tacaggagtg caaaggacag gatcataaaa
      181 agcagtggaa ttacaaacct caggtagttc cacatggtca ggaagaggtc ctcaattcca
      241 agaaagtctg aaacaaggct gccaataagc cttcctgcca caagagagac aaaaactata
      301 atgatcataa gaatcagaac cactgtaaac agtagactca atcccttaag ccttagaaat
      361 gacctgttct cagcataatt ataggccttg ttaattgcct ttatgaccgc ctttacccca
      421 gaggaagcag tccatagtcc tgcgatggct gcaatggata gaaaaccctg ggaacttgag
      481 ctaacaacat catcgtgaaa ggtctggatg atttgctgaa tatctgtggg catgtacttt
      541 ataaggtcca ttattacatc ctcacgacca agtggagtgt agctgatgac atttaggaga
      601 accatgagaa agggaaacag tgacaggatc aggaagtatg tcagttgtgc ccctgtaccc
      661 aatacattgt tttcagtaaa cctgtaggcc agctggtcca gaaagatcac tgaccgcttt
      721 ttcatgagct cttttatggt tttttccaaa atggtcacct gcctttaaaa aacgcctaat
      781 ctttaagatg cctatcaaac caatccacta tctcctttag cctgcgaatc cttccctgtg
      841 gccttccaac ccttgacaat ccatggcttt catttttgaa aaccaccatt tttgagtcga
      901 ctccatgata ctttaaggct gtatacatct gcaggccttc gggtatccaa caccggtaat
      961 ccttgtctga atgtatgaaa agggtaggag tctggacctg gtcagcatac ttaaggggtg
     1021 aattgtccca caaggctgag tagtccttcc agggacttgc tcccacctgg tctgttgcaa
     1081 agtagtatcc gatatccgag gtcccaaaga aggagaacca gttggaaatg ctcctctgtg
     1141 aaactgcagc cttgaacctc tttgtgtggc cgattatcca gttggtcatg tagcctccgt
     1201 aagacccacc agttacacca agcctttccc ggtctatgga tggaaatgtt tcaagtgcct
     1261 tgtctgtaaa gagcataatg tcctcatagt ctgtgttgcc ataattgccc cgtatgtcgg
     1321 agaattcatt tccgtagccg tcactacccc ttgggttcat atagaagaca aagtaaccct
     1381 ggtttgccca aagctgcatt tcatggtgaa agactccgcc gaatactgtc tttgggccac
     1441 catgtatgtg aaggatggcc ggataccttc tattcttatc aaaatctacc ggcttcagta
     1501 cccatccggt gagacttatc tcctcgttgt taaatgtaag aaccattggt gtcgatagct
     1561 tcctgtcctg gacaacccaa tcattgaagc ttgtaagcct gtgctcctgg tggtcaagtc
     1621 gatacaggtc ctgaaggtag aggtctctca ttcccacaaa gacaatggtt tcatccacca
     1681 catcaaaatc gtcaacagac ccgtcctcca ggctgacctg ttcaatattc ccattgaggg
     1741 ataccctatg gaggtggcag ctgtactctt ttgtgctaag gaaataaagg tggtcaccgg
     1801 aaaccttgaa actcctgttc attccatacc tgcagtcgga attaactgaa ctccaaaggg
     1861 aaaggtcagg ctcagcaatc cggacatagg tcaggccctc atcctctgtt acaaaaatac
     1921 gggggttttc atttattcca taggaagtca tatctgttcc cacaaagatt atcctatcgt
     1981 ccatatagtc agcataatag tagttgaagt ctgagattgg agaaatcttt tcacaggttc
     2041 cactggatag gctaagctga tacaggtggt tgtatggact catcttgtca gtgtaggagg
     2101 aagttacaag tatggccttt tcctttgaag ggtggattga aatgctttca acatttgtga
     2161 actcatcggt tatgggagtg agttcttccg tgctgctatc aaatatgtag caccttgttc
     2221 tcttcttgtt agtgaagtcg ccaccgtcgg tccagaaggg tatttcgtcc aggacctgat
     2281 attcgttctc cttctttagt gcctttactt tctcctcgat ctccttgtca tcaagaccct
     2341 gaatttcacc cagccttctg tcaaatgagc caagcagcag gtatttgttg tcctttatat
     2401 ggtcaaacct tgaaacgtca aggtatacct ggaaggcttt ctgtgcctct ccgccagtgg
     2461 ttgagatttc atagaagcag gttagggggt agcctttttc cttcttctcc ttatctgcct
     2521 tatccctgat ggaggagaag agcaacctgt ccttgccctt ccagacaaag gacccgtctg
     2581 agtcagatgc cgttagctgc cttgtaccat caatctcatc ccataggtaa atattggatc
     2641 tgtacttgtt ttcctcttca tccgccatgt gcaacaaaaa tccacaggtt cttccatctg
     2701 gtgaaatttg tgtccctgag agaaatttaa acctcgtcaa gtcctcccgc tttagattct
     2761 ccatagcctt cctccttgtt tcctttagta tattggccaa tattaataac aatgtttttt
     2821 ctgggcttgc tccattcctc aagatgtgta gccaccatcc ttccagttgg agtgattgat
     2881 accaacctat caaagcccct atttgataat atacccataa atgtcatata tcttaccaat
     2941 aaatacaggt catccgagtc aagaccgtcc agttcagcca gatcttcact gaaagtaggg
     3001 ccttccttaa gcctcagaac ggattgtaga aggagcttca tcatctgctt tgcattcctt
     3061 gaatccaatt ggaccaaccc ctttatggac tcctgattcc agaaacccct taggtggagg
     3121 gtgtacttgt cttctctggt catgccaagg tattcacttg cattggctgt cacggaaagt
     3181 gagttcttcc ttattttaat aagcttcaac atcaagccca tctggaaaaa gaaatgcaca
     3241 acaggttcct tcatctggcc aggcctactg aagtagaggg ggtaatcccc ttcaaggaag
     3301 tctattatat tgtgctggtc aaccttttta atgtggccct tcttttcagt tagttccagg
     3361 ggatagtcaa atacataaag aagaaacctg tcaaagtcgt tcaggtagct ttttatggca
     3421 tcggattcat ggtccttcaa ctggagggaa aggagaggat ctccctgagc cagggaattt
     3481 cttctgatta tttcctttag ataatgaaac ctgtccctgc ttatctggag gatctcctca
     3541 tatgcaccct ggtatttgct tgaaatcttg gacctgtaac tcaagtatgc ctttagggtt
     3601 gatatatagc tgttgaacac aaggcctgat gttatgaatt tctcataaat ggcatcccca
     3661 agcatgtcct tgattgaaat cttgtccata tccttgaagc tgtagccttc ctcgctgagg
     3721 tagtattcat agagctcgat catgttgcta aggtcctttt caatctgaga tgaacttttc
     3781 ttggttgaaa gatactcctc aaactcatcc agctcctggt agaccggaat aaggtcctcg
     3841 ccttgggggt cgaaatattt gtaggcgctt tggtagaaga ggtcatatat cttcagggca
     3901 tcatttttca ggtaggtctt ggttccctca gaacgttggt cctggtcaag cttgttgaag
     3961 tccctgatca gggttcttat aaatgaatgg attacggatt ttggaacaaa gctaacctct
     4021 ccgatcatcc tgctgttacc aagcactgtt cccaatcttg acaggaggta gtctcccacc
     4081 ttgaggacat aggaaacctg gggctcaagc aagctgtggt cttccccggt caaaacatcg
     4141 gttacgacaa cgtatggctc ctggaagtcc ttgatctgga agagggagac atatgatgca
     4201 gacttctccc ttaatatttc aacctcatcg ggagtgaggg aatcatcctc aagaatcagt
     4261 ctctcaccaa aggtcatgcc ctccttgtcc ctgtaatcgc agcaaagcca gatattgaag
     4321 ggaagcttgg cgctttcctc ctgtagaaag gggtggagtc caagcatcct gtcgtagatg
     4381 tacttgattt cttcctgttt gtttatatat tttatcaact tttcagtcgc cttgctttca
     4441 aggagctgaa gtctttcata ttcctttgta tgcataaaat cctccttatt aatacttaag
     4501 ccattatacc ccaagagaaa cacttataca ataaaagggg tgaaatcaaa acaattctga
     4561 aacttttaca gccatgacag gccatttcca tggcaatgaa aacattccct ggttaagatg
     4621 gcagggattc tggtatattg tagttagaca aacacgggag gtgtatgatg aaattcggag
     4681 attacaaata cgaaagaccc gacctggaca ggatggaaaa ggacatggac aggcttcttg
     4741 aaaacttcag gagggcagat ggaccagagg accagagtca ggtgatcaga aggataactg
     4801 aaataagaaa cagatttgag acaatgtaca cactggtaag cataaggcat tcaattgata
     4861 caagggatgc cttctatgaa aaagagcagg agttcatgga tgaaaattcc ccaaactata
     4921 caagtatggt caccaggttc tacagtgaac ttgtggggtc tcccttcaag gaagagcttt
     4981 cacgggaatg gggggaccag cttttcagac ttgcccacct tcagataaaa accttcagcc
     5041 aggaaatcat ggatgacctt gttgttgaaa acaagctgtc cacaaggtat gacaagctgg
     5101 ttgcagctgc agcaatccag tttgaaggca agaccagaaa cctcagccag cttcgaccct
     5161 ttatggaatc gccggacaga gacatgagaa aaagggccta cgaagcctac acatcgtatt
     5221 ttcaggagaa ggaagaggaa attggagaaa tctatgacca acttgtgaag gtaagggata
     5281 gaatggcaaa gaagctgggc tatgaaaatt ttgtccagat gggctatgac aggatgtcca
     5341 gatcggacta tgaccacagg atggttagga atttcaggga ccaggtactg gagaccgtgg
     5401 ttccagtggt aagcgaaatg aaggagagac aaaggatacg tcttggactg gaccagctga
     5461 cctattacga tgagcccttg ggctttttaa gtggcaatgc caacccaaag ggaggtccgg
     5521 aatggatcct ggaaaagggg atcaggatgt acaaggagct ttcagaggaa acaggtgaat
     5581 tctttgattt catggtggaa agggacctgc ttgacctcct gtccaaggag ggaaagagaa
     5641 gcgggggcta ctgtacggtt attccagatt acaatgcgcc cttcatcttt tccaacttca
     5701 acggtacaag cggtgatata gatgtgctga cccacgaggc aggccacgca ttccagacct
     5761 acatgagtat ggaccatgag cttcctgaat acaagtttcc aacccttgaa gcatgtgaaa
     5821 tccattccat gagcatggaa ttcttgacct ggccatggat ggatggcttt tttgaggaag
     5881 gtgtccacaa gtacaagtat agtcaccttg agggagctgt gagctttctt ccctatggtg
     5941 taaccgtaga ccattttcag catgtggtat atgagaatcc taacatgaca cccaaggaaa
     6001 gaaaggatgt atggagggac cttgaaagga agtacctccc atttaaggaa tatggagaaa
     6061 atgactttct tgaaagcgga ggatactgga tgaggcaggg acacattttc tcaaacccct
     6121 tctactatat agactattgc cttgcccagg tttgtgcatt ccagttctgg agaagaagcc
     6181 tggaggacag ggaaggggcc tggaatgatt accttgacct ttgcagggct ggcggcagca
     6241 tggcctttac agagctagtc catctggcca acctgaagga cccattcaag gacggcactg
     6301 tcaagtatgc cctggagcct gtaaaggatt ttcttgcaag catagacgac aaggaacttt
     6361 aaaaaaacca ccctggggtg gtttttaatc ttcttgattt tgattatcca aaagatagat
     6421 tatgtttttt agcaatcttt gcagatggta gttgaaaata atatccttct ccccgattat
     6481 ggcctttttc tcccagatag gagtgaagga atagagcttt tccataaggt ctatgttatc
     6541 cgggctgatg attgcaatgt gctgcatcat ctggagtgct gacaggtcac tgtagatgtc
     6601 atatattgtg ctaagggtaa tttcagaccc tggaaggtcc ctgttcctgg caagcttgat
     6661 ttttgtttcg ctttgaatta ggtcgaatat cttatcgatt ttggcaagtt cagacttgaa
     6721 atccttaagg tccaccttat acttgcccac aaccagttta taggcgtaat ctttggcacc
     6781 ggacatcatc ctctctgcat acttgtcaaa catcctgtag ctttttggag cggcaacaaa
     6841 gtaatttaca atgaccccta cggctattcc aacaaaggta tccagaattc tgcttacagc
     6901 atagggaacc atttcatact catggttaag ggaaaccgca acaaatacta ttgctgacag
     6961 ggagatcgcc tttttccacc ctaccagatt caggatatgg attaccagga tgataccaat
     7021 acccatgaaa atctggtttc ggggtaggta cagggcaaaa atgagggcaa taagaccgcc
     7081 gacaacagtt cccagaatac ggtttttacc ggcttcaaat gattcggaaa cagtggcctg
     7141 catggtatgt atggcaccga tcccaacgaa tagtggactt tgcaaattaa atacttcagc
     7201 aatgaagagt cctattgaca ctgccagggc agtttttatg gttcgcatcc caattctgta
     7261 tttttttaat accattacta ttctcctcat taatattctt tcattatact ctaacctata
     7321 cccttaaaat tatcaagagt ataatccaca aaataaaccc catcaaggtg cgttttccac
     7381 agagttgccc acattatcca cagagacttg tcaacttatc cacagaaacg ggggaatatt
     7441 atccacaagg ctccaggcct cttaagaccc agcatggaaa atactgacag gaaaacattg
     7501 tttagtaacc ggtaatctga gggtataata caaacaaata gtataagaga ttatactaca
     7561 tgaaaggagc tgggtttatg aaaatgaact atactggaaa aaacatggag gtaactgacg
     7621 cactaaggga cgtaactgag aagaaactga gcaagcttga aaagtatttt caatctgata
     7681 tctcaggaaa cgttacattc agcacagaaa agaacaggaa gatcatagaa gttaccataa
     7741 accttcccgg cacaatccta agggcagagg aatccagtga tgatatgtac gcatcaatcg
     7801 acaagactgt tgatgtactg gagagacagg ttagaaagca caagacaaaa ctgcagaaaa
     7861 ggtacagaaa caacgatacc ataagatttg aaaacgtgcc ttccctgacc aaggaagagg
     7921 aagaggacaa acctaagctg gtaagaagaa agagatttgg acttaagcca atgtcatcag
     7981 aggaggcaat acttcagatg gagctgttaa gacacaactt cttcgtattt atggatgctg
     8041 agtctgagga agtgtcagtt gtatataaga ggaaggatgg aaactacggg cttatcgagc
     8101 cagatttcaa ctaaacagtg gagatttcaa gcagggccta ggccctgctt ttttgatttg
     8161 gcacgattaa tgataaatgg tataattaaa tgatgttaat tttgagagga tgattttgat
     8221 gggactattt gacaaggttt ttggaaccta tagtgaaagg gagctgaaaa agcttgataa
     8281 aacggtggag cagattctgt ctctggagac ggaaatccag aatctaagcg acgatgcact
     8341 taagaacaag acaaatgaat tcaaggatag atttaagaat ggagaaggct taaatgaaat
     8401 aatgcctgaa gcatttgcag tagtgaggga ggcttcctgg agagtacttg ggatgaagca
     8461 cttccccgtt cagatatacg gtggaataat actccaccag ggccgtatag cagagatgaa
     8521 gacaggtgaa ggtaagaccc ttgtggctac actgcctgca taccttaacg caataacagg
     8581 tcttggagta cacgtaatta cggtgaatga ctaccttgca aagcgtgaca gacaatggat
     8641 gggcaagctt tttgaattcc tgggattgac tgtgggttgt gttgtccatg gaatgaacaa
     8701 caaggaaaag aaggaagcct acaaggccga cattacttac ggtacaaaca acgagttcgg
     8761 atttgactac ctaagggaca acatggtcat ctacaaggaa gagatggtcc agagaaaact
     8821 ttattatgct atagtggatg aggttgactc catacttgtg gatgaggcaa gaacgccact
     8881 tataatttcc ggaaagggtg acaagtcaac cagtctctac ggcatggctg attccttcgc
     8941 aaggacgctt tcctacagga agctggatcc cgaggagacc aagtcggacc ccttcaacag
     9001 ggagataaag gaagagactg ttgattacct gatcgatgaa aaggccaaga gcattgccct
     9061 tacagacaag gggactgcaa aggcggaaag tttctttgga atcgagaacc tggcagatgt
     9121 tgagaatatg gagacagccc accacatcaa ccaggcaata aaggcaagga gcataatgaa
     9181 aagggacata gattacgttg tccaggatgg cgaggtaata atagttgatg agttcaccgg
     9241 aagacttatg tatggaagaa ggtactcaaa tggtctgcac caggccattg aagccaagga
     9301 aggactggat gtaagaagcg aatccaagac ccttgccaca ataaccttcc agaattactt
     9361 caggatgtat gagaagcttt ccggtatgac cggtactgcc aagactgaag aggaggaatt
     9421 cagggaaatc tacaatatgg acgttgttga gattcctaca aacaagccgg tggtaaggat
     9481 tgacaatgcc gacagtgtat acaagacaga agccgggaaa ttcaaggctg ttgtggatga
     9541 gataagggaa aagcaccaga ttgggcagcc cattctggtt ggtacaataa ctattgagag
     9601 gtctgaactc ctatcgaaaa tgctaaagaa ggaagggatc aggcatgagg tccttaacgc
     9661 caagcagcat gagcgggagg ctgaaatagt tgcacaggca ggtagatttg ggtctgtaac
     9721 aatagcaaca aatatggccg gtagaggtac tgacatcgtc cttggtggaa atccagacca
     9781 tatggcaaag gatgaaatga agaaaaaggg ctatgacaat gaggtccttg cctatgtcga
     9841 cggattcttt gagaccgatg accaggaggt tattgaggca aggaaagtct atcaggaact
     9901 tgtgaaaaag catgaggggg aaaccagggt cgagcacgaa aaggtggtcc aggctggggg
     9961 tcttcatata atcggtactg agagacatga gtcaagacgt attgacaacc agcttagggg
    10021 tagatctgga cgtcagggag accctggaag ctccaggttc tacatctcac ttgaggatga
    10081 cctgatgagg ctatttgccg gtgacaggat aaagggaata atcgatggca tgaacatgcc
    10141 tgaggacgag ccacttgagc atgccctcct tacaaagacc atagagggag cacagaagaa
    10201 ggtagaggga aacaacttct ccatccgtaa gcacgttctc cagtatgacg atgtaatgaa
    10261 caagcaaagg gagatcatgt acggtgagag aaggaaggta cttgaagggg aaaacctcaa
    10321 ggaccacata caggaaatgg tcagaggcct tgtagttgat aatgttgagc tttacacaag
    10381 gggggagaag tatccggaac agtgggattt cgagggtctt gaaacctacc ttgtgaagca
    10441 gttcggcttt aggcagggaa tattcgccgg catggacatt gaatctctaa ccaaggaaga
    10501 tttgatagat aggatcatga agaagtcgga ggaggcctac aaggtcaagg aagccgagtt
    10561 tggagaggac cagttcaggg agattgaaag ggttgtcctc cttcaggttg tggacaggaa
    10621 atggatggac cacatcgatg caatggacca gcttagacag gggattggac ttagggccta
    10681 cggtcagcag gatccggtga gggcttacca gattgagggc tttgacatgt ttgaggagat
    10741 gaccagaagc atctgggagg atactgttgg acttctatac catgtggtca aaaatga
//
LOCUS       DASRQF010000061         2418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1701161, whole genome shotgun sequence.
ACCESSION   DASRQF010000061 DASRQF010000000
VERSION     DASRQF010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701161"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..283)
                     /locus_tag="VFD79_01565"
     CDS             complement(77..283)
                     /locus_tag="VFD79_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HZH92764.1"
                     /translation="MKKTMMVEGMSCGHCEMAVKNALKELEGVQDVNVDLSTGKVEVE
                     GDNLLDNLLMEAVDEAGYSVKEIG"
     gene            complement(300..>2418)
                     /locus_tag="VFD79_01570"
     CDS             complement(300..>2418)
                     /locus_tag="VFD79_01570"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZH92765.1"
                     /translation="SSCSARVEKALNKLEGVTSANVNLSTNKAVVVFPSGVLEDSQII
                     EAIEKAGYKAEVERDVSSDREKELREKEIKSLKRSFIISAILTLPLFSAMFFHMAGQM
                     NILTNGYFQWVLATPVQFLIGARFYKGAYKSLRGGGANMDVLIAMGTSAAYFYSVYNI
                     FAGVHEYYFEASAVIITLILLGKTFEAVAKGKTSEAIKKLMGLQPKTARVLKDGEEKE
                     IPIENLQIGDIIIVKPGEKVPVDGVIVQGSSSLDESMITGESIPVDKGEGDQVVGATI
                     NKFGSFRFEAQKIGKDTVLSQIIKLVEDAQGSKAPVQRLADKISGIFVPTVIGIALVT
                     FIAFYFIDSFNTGLINAVAILVIACPCALGLATPTAIMVGTGKGAENGILIKSGEHLE
                     RAHKMDSIIFDKTGTITKGKPDVTDIVSFGEDRKDILRISASVEKTSEHPLGEAIVKK
                     AEEEGLALSEAESFSAVPGKGLKASFEGREILLGNRKLMRENDIDLTDREKDIEKLET
                     EGKTAMILAVDGRLTGIIAVADGIKETSQAAIRELQDMGLKVFMITGDNERTARAIGA
                     QVGIENILADVLPEDKASEVEKLKSQGMHVGMVGDGINDAPALAAADVGFAIGTGTDV
                     AMEAADITLMRGDLEGVVTAMRLSHRTMKTIKQNLFWAFFYNTLGIPFAALGFLNPMV
                     AGAAMAFSSVSVVSNSLRLRNFK"
BASE COUNT          519 a          660 c          478 g          761 t
ORIGIN      
        1 ttccatctac ctgtccccta acggttttaa gctttttaag agctgtctcc ttcccctggt
       61 tcaccttgac taccccctat ccgatctctt taactgagta gcctgcttcg tctacagcct
      121 ccatcagaag attgtctaga agattgtctc cctctacttc aaccttgcct gttgacaggt
      181 ccacatttac atcctgaaca ccttcaagct cctttagggc gttctttacg gccatttcgc
      241 aatgtccaca gctcatgcct tcaaccatca ttgttttttt catttttgtc tcctcctgtt
      301 tatttgaaat ttctaagcct taacgagttt gatacaactg atactgaact gaatgccatt
      361 gcagctcctg caaccattgg attcaggaat cccagtgcag caaagggtat tcccagtgtg
      421 ttgtagaaga atgcccagaa gaggttttgc ttgattgtct tcattgtcct gtgactgagt
      481 ctcattgctg taacaactcc ctcaagatcg cctctcataa gggttatgtc cgccgcctcc
      541 attgccacat ccgtacctgt tccgattgca aagccaacat ctgccgctgc aagggccggg
      601 gcgtcattta tgccgtctcc taccattcca acatgcatcc cctgcgactt gagcttctca
      661 acctcacttg ccttgtcctc aggaagtaca tccgcaagaa tgttctcgat tccaacctgg
      721 gctcctatgg cccttgctgt cctttcgttg tctccggtta tcatgaatac cttaagaccc
      781 atgtcctgaa gctccctgat ggccgcctgg gatgtttcct tgataccgtc tgcaacagct
      841 atgattcctg tcagcctacc gtcaacggct agtatcatgg ctgtctttcc ttctgtttca
      901 agcttttcaa tatccttttc cctatcagtt aaatcaatat cattctccct catgagcttc
      961 ctgttaccga gaagaatctc cctgccttca aagcttgcct taagtccctt tcctggaacg
     1021 gctgagaagc tttccgcctc acttagggcc agtccttcct cttcagcctt cttgactatg
     1081 gcctctccca ggggatgctc agaggtcttt tcaacagagg ccgatatcct gagaatatcc
     1141 tttcggtctt caccaaagct tactatatca gttacatcgg gttttccttt tgttattgtt
     1201 cctgtcttgt cgaatattat tgagtccatc ttatgtgccc tttcaagatg ttcccccgac
     1261 ttgatcagga ttccattctc ggctcccttg cctgttccaa ccatgattgc cgttggtgtg
     1321 gcaagtccca aggcacaggg acatgcgatt acaagtattg caactgcgtt tatgagccct
     1381 gtgttgaagc tgtctataaa gtagaatgca atgaaggtga ccagtgcaat tccaattacc
     1441 gttggtacga aaataccgga tatcttgtct gccagccttt gaaccggggc ctttgagccc
     1501 tgggcatctt caacaagctt gattatctgt gaaagtactg tatccttgcc gattttttgt
     1561 gcctcaaacc tgaaggaccc aaacttgttt atggtggctc caaccacctg gtccccctcg
     1621 cccttgtcaa ctggtatgct ttcccctgtg atcatggact catccaggga tgaactaccc
     1681 tggactatga caccgtcaac aggtaccttt tctccgggct tgactattat tatatcccca
     1741 atctggagat tctctattgg gatttccttc tcttccccgt cctttaggac ccttgctgtc
     1801 ttgggctgta gacccataag cttcttgatg gcctcagatg tcttgccttt tgcaacagcc
     1861 tcaaaggtct ttcctagaag tatcagggta attatgaccg ctgatgcttc aaagtagtat
     1921 tcgtgcactc ctgcaaaaat attgtagacg ctgtagaagt aggctgcaga tgtacccatt
     1981 gctataagga catccatatt tgctccccca cctctaaggg acttgtaggc acccttgtag
     2041 aacctggctc cgatcaggaa ctgtacggga gttgccaaga cccactgaaa gtatccattt
     2101 gtcagaatgt tcatctggcc tgccatgtga aagaacattg ctgagaatag gggcagggtc
     2161 aatattgctg agatgataaa ggatcgtttt agtgacttga tttccttttc tctcagttcc
     2221 ttctccctgt ctgatgatac atccctttct acttccgcct tatagccggc cttttcgatt
     2281 gcttcaatga tttgtgaatc ctcaaggacc cctgaaggaa atacaaccac tgccttgttt
     2341 gtagataggt ttacatttgc tgaagtaact ccctccagtt tgtttagggc tttctccacc
     2401 ctggctgagc aggagctt
//
LOCUS       DASRQF010000062         6632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1581524, whole genome shotgun sequence.
ACCESSION   DASRQF010000062 DASRQF010000000
VERSION     DASRQF010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6632
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581524"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VFD79_01575"
     CDS             complement(<1..888)
                     /locus_tag="VFD79_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92766.1"
                     /translation="MKEKFKTILLFLLVGTSLAMTQQLWMQFPERVTGFFKPETSYSS
                     SYLLSDMIAPNKYLVGIGGNNYTMVYDANKYGIWDTSKESLAGLLRSDTIRIEESETG
                     YSPGQAVGMTVVFYFPESISTYILAKAWNVNEPNEIVDTIPYVDRISIVKGSGDPFFV
                     FSDEERDVLVYDSGVAVDELQSDILDFEDSADFDYYYSLSEIFPTDVGDIYVPYEIRH
                     SLPVVYVSNDVAQMTSGEMGILAERFLEREIDYIRQIVESNGSTIYIYNNKVLKFNMN
                     GTVEYFHSLEDTIQERNLYL"
     gene            complement(911..1411)
                     /locus_tag="VFD79_01580"
     CDS             complement(911..1411)
                     /locus_tag="VFD79_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92767.1"
                     /translation="MFKRTLASLMIMMAVLGFASFAHASTVTTGNAISTRTDYEIIKP
                     EKEIYSSSERVVLINGRAPSGTEITVELYGTTDLTRRSFNLDRLPAHEDYILIKSDSV
                     TSGNLGFFQKQMDLVRGINKLVIDFGVEGVEDKEFIVFKYDVRRTDIRLPYDRESGGI
                     RMPLIR"
     gene            complement(1499..3286)
                     /locus_tag="VFD79_01585"
     CDS             complement(1499..3286)
                     /locus_tag="VFD79_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZH92768.1"
                     /translation="MFSSIRWKFIVVYFLLVFIAMVIIGIFIVNRMENQQLETARSSM
                     EQQIETIMSTSSYIATGSWTENREGIQGTLNDWRLGTGETLYVIYDEDVPKILATTNK
                     QYESIVGNNALSYRYTDPTIIGRAFNGERAQSVIMDDVAGNEFMHYAAPVFSSVGKIN
                     GILYMTSDLSNVQRTLEDSRNILTNATLIALGVTIVLGFLIASSITGPIRDVTKKAEE
                     MALGDFDQFVEVKSDDEIGQLAGMFNHLTLKLKDTIEEMDLERSKLDTIFSYMAEGVI
                     AVDKNGSIIHANPIGQKLTEIDDEYLDRSERFDLEKLGMENIDYTDPETLSGETTFQK
                     GDGVYKVKYAPYKNERDHIAGVIMVLQDITQEHRLDIMRKEFVANVSHELKTPITTIK
                     SYAETLMTNEVDPDTQQRFLSVIDRECDRMGRLVRDLLQLSNIDYQKTKWNRENISIG
                     DMLLDSINKLQLLAHEKNQRIETYIQPDLPQIKVDRDGMEQVVLNILSNAIKYTENDG
                     TIKVSAKTRDHFIDLSISDNGIGIPEEDVGRIFERFYRVEKGRSRDMGGTGLGLSIAK
                     EIIEAHNGTIKITSKKDRGTHVRITLPVV"
     gene            complement(3288..3980)
                     /locus_tag="VFD79_01590"
     CDS             complement(3288..3980)
                     /locus_tag="VFD79_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZH92769.1"
                     /translation="MNNKILVVDDEKAIGDIIKFNLEKEGFQVTVAYDGEEGLDKIKL
                     TQPDLLILDIMMPKKDGFQVLKEIRPTFRFPVIVLTAKEEEVDKVLGLELGADDYMVK
                     PFSMRELVARVKANIRRLDYDLSAGNGAAEITSEDLEIDFTKYEVRKRGKVVDLTLRE
                     FELLKFLALSAEQVFTREQLLEKVWGYEYYGDIRTVDVTVRRLREKIEDDSGEFKYIL
                     TKRGVGYYFRRV"
     gene            4276..>6632
                     /locus_tag="VFD79_01595"
     CDS             4276..>6632
                     /locus_tag="VFD79_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005957977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HZH92770.1"
                     /translation="MEWYQKKPQEIAEELKVSLSAGLSNEEAKNRLDQYGPNEMKEEE
                     GKSLLSKIIAQFSDFLILILMSAALISVFVGETKDAIVIMSIVVVNAMLGLYQEGKAE
                     KALESLKKMASPTAKVIRGGKTQEISASDLVPGDLVVLETGDIIPADLRLVESSNLKV
                     EEASLTGESVPSEKRSTMELTDETPLGDRRNMAYMSTIVTYGRGNGLVVETGASTEIG
                     KIATMIQSIDEESTPLQKKLNHLGKVLGITVILVCIAVFGIGMIQGRDLLEMFMIAIS
                     LAVAAIPEGLPAIVTIVLAIGMNKMVERNAIVKKLLAVETLGATSVICTDKTGTLTQN
                     EMTVVKLYTNDRVVDVEGRGYEPEGKFLVDSNEIKVEELKGLNLLLTISALVNDAKLE
                     EKDDQFKILGDPTEGALLTLSEKGGIRSKTINGKYPRVKEIPFDSERKMMTTFHEMAG
                     KDEIVSFTKGAPDIIIDRCDKILLDGDVVKLTDEVRQKVLDQNTAFSRDALRVLALAF
                     REYQRVPEDPTSEDHEKDMIFVGLTGMIDPPRPEAREAIAKCKNAGINTIMITGDYRE
                     TAFAIAKDLGLADREDQAIMGSKIDSISDEELREVVKETKVFARVSPEHKVRIVTALR
                     ANGDIAAMTGDGVNDAMAIKKADIGIAMGITGTDVAKNTADMILTDDNFASIVAAVEE
                     GRIIFSNIKKFVYFLLSCNIGEILLVFVSILLNLPVPLLAIQLLWLNLVTDSFPALAL
                     GVEKGEPDIMDRPPRDPNEAILDKRMLLGVSFQSLAVAGASLAAYL"
BASE COUNT         1747 a         1545 c         1409 g         1931 t
ORIGIN      
        1 caggtagaga tttctctctt ggattgtatc ctccaatgag tggaagtatt caactgttcc
       61 attcatattg aacttcaata ccttgttgtt gtagatgtag attgtggacc cattgctttc
      121 cacaatctgt ctgatatagt caatctccct ttcaaggaat ctttccgcca gtattcccat
      181 ctctcctgaa gtcatctggg caacgtcatt gcttacatag acaactggca ggctgtgcct
      241 gatttcatag gggacataga tgtcccccac atccgtggga aatatctcag atagggagta
      301 gtagtagtca aagtcggcac tatcctcaaa atccaatatg tccgattgga gttcatccac
      361 tgcaactcct gagtcatata ccaaaacatc ccgttcttca tctgaaaaaa caaaaaaagg
      421 atccccacta ccctttacta tggaaattct gtccacataa ggtatggtat ccactatttc
      481 atttggttca ttaacattcc acgcttttgc caatatatat gtgcttatgc tttcagggaa
      541 gtagaacaca actgtcatcc ccaccgcctg accggggctg taacctgttt cagattcctc
      601 tatcctgatg gtgtccgacc ttagcaaccc tgcaagagac tcctttgaag tatcccaaat
      661 tccatacttg ttggcatcgt acaccattgt gtagttattc ccgccaatac ctaccaggta
      721 tttattggga gcaatcatat cagacagcaa gtatgatgaa gaataggatg tttcaggttt
      781 gaaaaaccct gtcactcttt caggaaactg catccacagc tgctgtgtca ttgccaggct
      841 tgttcccacc aaaagaaaaa ggagaatggt cttaaatttt tccttcattc aaacctctcc
      901 tcttgttttt ctatctaatt agcggcattc taatcccgcc tgactcccta tcgtatggaa
      961 gcctgatatc tgttcttctt acgtcatatt taaatactat gaattccttg tcttcaactc
     1021 cctcaactcc gaagtcaata acaagcttgt tgatccctct aacaagatcc atttgcttct
     1081 ggaagaaacc aagatttcca gaagtgacac tgtcgctttt tatcaatata taatcttcat
     1141 gggctggtag tctgtccagg ttgaagcttc ttctggtcaa atctgtggtt ccgtaaagct
     1201 ccactgttat ttcagttccg gatggtgctc taccgttgat aagaacaact ctttcacttg
     1261 aagaatagat ttccttttcc ggctttataa tttcgtaatc agttctggtt gaaattgcat
     1321 ttcccgtagt tacagtggat gcatgtgcaa agcttgcaaa accaagaact gccatcataa
     1381 tcatcaaact ggccaatgtt cttttaaaca taccctaacc ccctttatct gcatattctt
     1441 tcatttgcat tatacccctg gaatgttaca aacatattac agcagggtta aaatagtgtt
     1501 acacaaccgg taatgttatt ctgacatgag tcccccggtc ttttttactt gtgattttta
     1561 tggttccatt gtgtgcctca attatctctt ttgctatgga aagtccaaga cctgtgccgc
     1621 ccatatccct gcttcttccc ttttcaaccc gatagaatct ttcaaatatc ctacccacat
     1681 cctcctcggg gattcctatt ccgttgtcgg atatgctaag gtctatgaaa tggtccctag
     1741 tctttgcact gactttgatg gttccatcat tttcagtgta cttgattgca tttgaaagta
     1801 tgttcagcac tacctgctcc ataccatccc tgtccacctt tatctgggga aggtctggct
     1861 ggatgtaggt ctcgattctc tgatttttct catgggcaag gagctgaagc ttgttaattg
     1921 agtcaagcag catgtcccca atggagatgt tctccctgtt ccactttgtc ttctggtagt
     1981 ctatgtttga aagctgaagc aggtccctta caagtcttcc catccggtca cactccctgt
     2041 ctatgactga caggaatctc tgctgggtat ccgggtccac ctcattcgtc atcaaggtct
     2101 ccgcatagct tttgatggtt gtgattggcg tcttaagctc atgggataca tttgccacaa
     2161 actcctttct catgatgtcc agcctgtgct cctgggtgat gtcctgaagg accattatca
     2221 caccggcaat gtggtccctc tcatttttgt agggggcata cttcaccttg tagacaccat
     2281 cccccttctg gaaggtggtt tctccactga gggtttcagg gtctgtatag tctatgtttt
     2341 ccatgccaag cttttcaagg tcaaaccttt ccgacctgtc aagatactca tcatcgatct
     2401 ctgtaagctt ctgaccaatt gggtttgcat ggattatgct gccgttcttg tccacagcta
     2461 ttactccctc ggccatgtag ctgaatattg tatccagctt gctcctttca aggtccatct
     2521 cctcgatggt gtcctttagc ttaagtgtaa gatggttgaa cattcctgcc agctggccga
     2581 tctcatcatc ggattttacc tcaacaaact ggtcgaagtc tcccagggcc atctcctcag
     2641 ctttttttgt aacatcccgg attggacctg ttatgctgct tgcgataagg aagcccaaca
     2701 cgattgtcac acccaaggca ataagtgttg cattggtaag gatattcctt gagtcctcca
     2761 gggtcctttg cacattagaa aggtcagatg tcatgtagag tatgccgttt atctttccta
     2821 cactggaaaa tacaggggct gcataatgca taaactcatt tccagctaca tcatccatta
     2881 tgacagactg tgccctttca ccattgaagg cccttcctat tatggtaggg tctgtgtacc
     2941 tgtatgacag ggcattgttt cccactattg attcatattg cttgtttgtg gtagccaata
     3001 tcttgggaac atcctcatca tagataacat agagcgtttc cccggtacca agtctccaat
     3061 cattaagggt tccctgtatg ccctctcgat tctctgtcca gctcccagtt gcaatgtagc
     3121 ttgaggttga cattatggtt tcaatctgct gctccattga agaccttgct gtttccagct
     3181 gctgattctc catcctgttc actatgaata ttccaattat aaccatggca atgaaaacca
     3241 gaaggaaata caccacgata aatttccacc tgatgctgga gaacatatca gaccctcctg
     3301 aaatagtatc caacgcctcg tttggttagt atatacttga actcccctga gtcgtcttca
     3361 attttctctc ttaaccttcg cactgtcaca tcaacggttc ttatgtcacc gtagtattca
     3421 tatccccaaa ccttctcaag aagctgctct cttgtaaaaa cctgttctgc actcagggcc
     3481 aaaaacttta ggagctcaaa ctccctcagt gtaaggtcga caaccttacc tctttttctt
     3541 acctcgtatt ttgtgaaatc tatctccagg tcctcggatg tgatctcagc agcaccattg
     3601 cctgctgaaa ggtcgtaatc cagtctcctg atgtttgcct tgacccttgc cacaagctcc
     3661 ctcatgctga atggtttgac catgtaatcg tccgcaccaa gctccagacc aaggaccttg
     3721 tcaacctcct cttccttggc agtaaggact atcaccggaa acctgaaggt tggccttatt
     3781 tccttgagca cctggaagcc atcctttttg ggcatcatta tatccaatat tagaaggtct
     3841 ggctgagtaa gctttatctt atcaagaccc tcctcaccgt catatgccac tgtaacctgg
     3901 aagccttcct tttccagatt gaactttatt atatctccta tagctttttc atcatcgacc
     3961 acaagtatct tgttgttcat atgaatcaca cctttttctc atttatttaa gtaaattatc
     4021 ccctaatcgg tccaataagt caatgcttag gagctctgtg tgcaatttta gatttatctt
     4081 gctttttagc tttttgagta ctataatagg tctaacaaaa gggagtagct aatctgtgat
     4141 aatcgtcatt acggcctggc ccggttatca ccttcattga agtagcaaga cttttgtcca
     4201 tttaagggtg ggcgaaagtc tttgtcattt tttgggtata tagttactgc ataacaaagt
     4261 gaaagaaggg atagaatgga atggtaccaa aagaaacctc aggagatagc tgaagagctg
     4321 aaggtcagtc tgtctgccgg actctcaaat gaagaagcaa agaataggtt agaccaatac
     4381 ggtcccaatg aaatgaagga ggaagaggga aagagcctgc tatcaaaaat cattgctcag
     4441 ttttccgatt ttttgatact tatacttatg tcagctgcac tcatatctgt ttttgtgggt
     4501 gaaacaaagg atgcaattgt aatcatgtcc attgttgtag taaacgccat gctgggactc
     4561 tatcaggagg gcaaggcaga gaaggcattg gaatccctta agaagatggc ctcacccaca
     4621 gctaaggtaa taaggggtgg aaagacccag gagatttcag catcagacct tgtaccgggt
     4681 gatctagttg ttcttgaaac cggagacatt ataccggcag acctgagact ggttgaaagt
     4741 tcaaacctaa aggtggagga ggcttcccta acaggagagt ctgttccctc ggagaaaagg
     4801 tcaacaatgg aacttacaga cgaaactccc cttggagaca ggcggaacat ggcatacatg
     4861 agcacaattg tcacctacgg acgtggtaac ggcctggttg ttgagactgg tgcttccaca
     4921 gagataggca agattgcgac catgatacag tccatagacg aggagagtac accacttcag
     4981 aagaagctta accatcttgg aaaggttctg ggaataacag ttattctggt gtgtatagct
     5041 gtttttggaa ttggaatgat ccagggaagg gacctgctgg agatgttcat gattgccata
     5101 agtcttgcag ttgctgcaat tcctgaaggt ctacctgcca ttgttacaat cgttctggcc
     5161 atcggcatga acaagatggt tgagagaaac gcaattgtga aaaagctcct tgcagttgag
     5221 actttagggg ccacctctgt catttgtact gacaaaacgg gcacactcac ccagaacgag
     5281 atgacagttg tcaagcttta taccaacgac cgggttgtgg atgtggaagg aaggggttat
     5341 gagcctgaag gaaaattcct tgtagacagc aatgaaatta aggttgaaga acttaaaggc
     5401 ctaaaccttc tccttacaat ctcagccctt gtaaacgacg caaagcttga ggaaaaggat
     5461 gaccagttca agatacttgg ggaccctaca gagggtgcac tacttaccct gtcagaaaaa
     5521 ggtggcataa ggtctaagac cataaatggc aagtacccca gggtcaagga gatcccattt
     5581 gattccgaaa ggaagatgat gacaaccttc catgaaatgg ctggcaagga tgaaatcgta
     5641 tccttcacca agggagctcc ggatattata atagacagat gtgacaagat actccttgac
     5701 ggggatgtag taaaacttac ggatgaggtc agacagaaag ttctggatca gaacactgcc
     5761 ttctccaggg atgccctcag ggttctggcc ctggcattta gggagtacca gagagttcca
     5821 gaagacccca cctctgaaga ccatgaaaag gatatgatat ttgtaggtct tacaggtatg
     5881 attgaccctc caagaccgga ggctagagag gcaattgcaa aatgcaagaa tgccggaatc
     5941 aataccatta tgattacagg agactacagg gaaactgcct ttgctattgc caaggacctt
     6001 ggtcttgcgg acagggaaga ccaggcaatc atggggagta aaattgattc catctcagat
     6061 gaggaactga gggaagtggt caaggagaca aaggtctttg caagggtttc accagagcac
     6121 aaggtgagga ttgtaacagc tttaagggca aacggcgaca tcgctgccat gaccggcgat
     6181 ggggtgaacg atgcaatggc cataaagaag gctgacattg gtattgccat gggtataacc
     6241 ggaacagatg ttgccaagaa taccgcagac atgatattga ctgatgacaa ctttgccagc
     6301 atagttgcag cggttgaaga ggggagaata atattttcca atatcaagaa gtttgtctac
     6361 ttcctcctat cctgcaacat cggagagatt ctcctggtgt ttgtcagtat cctgcttaac
     6421 ctgcctgtac ccttgctggc cattcagctt ctgtggctaa accttgttac agacagcttc
     6481 cctgcattgg cacttggggt tgaaaagggt gagcccgata ttatggatag accgccaaga
     6541 gatcccaatg aagcgattct cgacaagagg atgctccttg gagtctcctt ccagagcctt
     6601 gcagttgcag gggcctcact ggcagcctac ct
//
LOCUS       DASRQF010000063         8557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1578635, whole genome shotgun sequence.
ACCESSION   DASRQF010000063 DASRQF010000000
VERSION     DASRQF010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 8557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8557
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578635"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VFD79_01600"
     CDS             <1..649
                     /locus_tag="VFD79_01600"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HZH92771.1"
                     /translation="SGHLARRIGKTLMKWRRKIMILNILLFDDFETLDVFGPVEVFGN
                     LPKDYELRYLSMEGGIVKSRHGVKVLTEALESFSPDGIIFIPGGYGVRPLVGDPAYVE
                     MVKGLCEKSQFCLTVCTGSALLAKTGLLKGLLATSNKLSFDWVVSMDPGVDWQSMARW
                     AVHGKYWTSSGVSAGIDMALAFVEKANGRDEAERVARFMEYIWNEDNDNDPFEIG"
     gene            complement(679..1971)
                     /locus_tag="VFD79_01605"
     CDS             complement(679..1971)
                     /locus_tag="VFD79_01605"
                     /inference="COORDINATES: protein motif:HMM:NF025159.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4179 domain-containing protein"
                     /protein_id="HZH92772.1"
                     /translation="MTRLDRMLEEKRTEYNNLQAPDSLEEKLRAALDDEKTRRKFPLS
                     RIAAIFLVGAILTYNSSTLAFYGKQLIGYDTVMDGTLLQLSRMGEGQIIDKSYLFSDG
                     IRVQVDGVMLDGNGMVFFYTIEDTTKSKNMDDVYVRVSPDIPFMNVGSSGAGEIIKEE
                     HMQKWVFTTDEPPPFFVRKMTLKLSNERGDGMWEEGEIDFKLDRNKAVGETIRVPVGR
                     KVELAGRTMTVEDMSMSAISTVVKGEIQDLMSLGMDRLNEEYMMPHNMEMSLYADGIE
                     IDRKGAGMSTDMKGTRFDVRFDALPKDTKDLRLDLDSVSVHERLDMELEIAEGETLEI
                     RGNDIFINSIENGNGRTYITITTEEGTRIPGVRLLADGVYYDLLKTSESTYEKKDEDI
                     LNTRTLEFDGSGEDIKLSLTDISYTKTYNQTLYEESIE"
     gene            complement(1955..2482)
                     /locus_tag="VFD79_01610"
     CDS             complement(1955..2482)
                     /locus_tag="VFD79_01610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZH92773.1"
                     /translation="MELKYLVKKAKKGDKEALLKLILQERDNYYRLAFVYMRNQQDAE
                     DMVQDMILSLYDNVKNLRKEESFYSWSKTILVNLCKGRLETRKRVIPVEGVKEESIEF
                     IGGEDRMDLDYYLGTLKESHREIIRLRYYLDMDYKSISELLEIPLGTVKSRVNTAMDK
                     LRSAMGEENLDDQAR"
     gene            complement(2581..2925)
                     /locus_tag="VFD79_01615"
     CDS             complement(2581..2925)
                     /locus_tag="VFD79_01615"
                     /inference="COORDINATES: protein motif:HMM:NF007494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HZH92774.1"
                     /translation="MTVKNQVTFDDFLKLDIRVGKIIKAEVFEKARKPSYKLLVDMGP
                     EIGEKNSSAQITEHYTPEDLIGKQVLAVVNFPPRNIAGFMSEILVMGTYSQGGVVLIS
                     PDKPVQPGDILG"
     gene            complement(2927..3433)
                     /locus_tag="VFD79_01620"
     CDS             complement(2927..3433)
                     /locus_tag="VFD79_01620"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HZH92775.1"
                     /translation="MNLIFAVDGNWKIGFNGQMLVEIEEDLHRFRRITEGHIVIMGRK
                     TLEALPDQKPLPNRINILVTRNKDYKPEGFLVVNSLEDLFPLLKEVNPDRKMEVFVTG
                     GGTIARQLLLFCNKAYITKIFKDFRNTDTFIPNLDLDDDWDVVWESEVQQQDDIYYRY
                     INYERVRW"
     gene            complement(3444..4538)
                     /locus_tag="VFD79_01625"
     CDS             complement(3444..4538)
                     /locus_tag="VFD79_01625"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HZH92776.1"
                     /translation="MIIRGKHGEAEIFTENLEPGAKDQIERFLDQDYMEDKKVRVMPD
                     VHQGMGCVIGLTAQMGDIVIPNIVGVDIGCGMLLVKLEDRDLPLEDLDRFIRKNVPSG
                     TKTHRDNRFTDPEIEKLKCLKSLKGRERFQRSLGTLGGGNHFIELGRDSKGTLYLTIH
                     SGSRNLGKQIADHYQKLAQKSCKGDYPKDLCYLTGKERDDYLHDMKIAQNYAKINREG
                     IAELILLEFFGESLDNFQHFHTVHNYIDFRDNITRKGAISAYEGQELLIPINMKEGSL
                     LARGKGNEDWNFSAPHGAGRILSRRDAKEKASLEEFRRSMEGIYSTSVHEGTLDESPL
                     AYKPLEEILQRLDETVEIIERIIPIYNFKA"
     gene            4678..5256
                     /locus_tag="VFD79_01630"
     CDS             4678..5256
                     /locus_tag="VFD79_01630"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HZH92777.1"
                     /translation="MKKLIILMFALIMAVALAACGGSQEPEEPAEPPVVEEPAEEPEE
                     PAQEPEEQTAEVILYFANNQYVETGDEQYEWMLTETRTIAYDDEVCLEEAVVRALMEG
                     PEDEENLSTGFPDSVQLIGVSVLDGTAYVNFSSEGLGGGSMQESYVISQTVESLGELG
                     SVQRVQFLVDDQEAESLMGHISIEEPIEVTVE"
     gene            complement(5296..6147)
                     /locus_tag="VFD79_01635"
     CDS             complement(5296..6147)
                     /locus_tag="VFD79_01635"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZH92778.1"
                     /translation="MNKFHEGSLIYPLNLSLNVMDMDLMTRFYKDSLGLEILEKKNDF
                     VDLGVDGRRILTLVRPENVIQKPPRRTGLYHFAILLPTRKHLGSFINHLRDANVPVTG
                     GADHEVSEALYLQDPEDNGIEVYADIPDSKWEWDGDQVQMGTDPLDFRGLMELGRDTP
                     WTGMPEGTRLGHMHLHVGDLEEAYRFYGLMGFKRVAQLAGSANFISTGGYHHHIGLNV
                     WNGRGAKPAPENATGLRHYTLAAPDSEALEKIFESLQGENYQAEMEDGVLFTKDPSGN
                     RIQIIIP"
     gene            complement(6214..6402)
                     /locus_tag="VFD79_01640"
     CDS             complement(6214..6402)
                     /locus_tag="VFD79_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92779.1"
                     /translation="MKNRKGIVVLIVSLVVLGLIYLVLPDRIAVLFWLTGDFVYAPRE
                     FVFVFALIPYVVYRTWWE"
     gene            complement(6421..7605)
                     /locus_tag="VFD79_01645"
     CDS             complement(6421..7605)
                     /locus_tag="VFD79_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZH92780.1"
                     /translation="MATSLGIITILGLTANYLMEKVKIPGLLGMLLVGVLLGPGGFDF
                     ISPDLMRVSADFREIALIIILLRAGLGLDWEVLKKVGRPALGLSFIPGVLEGLTVMVV
                     STVFFGFSMVQGGILGFIIAAVSPAVVVPAMLQLNDEGRGNDKNIPTLILAGASLDDI
                     VAITIFTSFLGLYGGENVSLAGQLMGIPISIITGVGLGYLMGHVFVRIFETQHIRDTK
                     KVLMILGAAILLMGLEDLIRDTIAFSGFLGVMTIGFVILLKRGIVAKRLSVKFNKIWI
                     LAEILLFVLVGAQVDLGVTLDAGGAGILIIAVGLLMRSLGVYIALLGTDLNRKEKAFC
                     AVAYTPKATVQAAIGAVPLAYGVQGGDLILSMAVLSIVVTAPLGAVGIRYMKRLLSIL
                     KH"
     regulatory      complement(7660..7718)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(7762..>8557)
                     /gene="thrB"
                     /locus_tag="VFD79_01650"
     CDS             complement(7762..>8557)
                     /gene="thrB"
                     /locus_tag="VFD79_01650"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HZH92781.1"
                     /translation="MGLAIDRYNEFSFKPNAGSTKISSMVHQGYNMVFEHLGRDPEPV
                     EITIDAEIPIARGLGSSSSCIIGGMMGANEVLGRPLERKDILRIATYMEGHPDNVTPA
                     LLGGVVISAMEEGEVYSIRIPLKNTYDFITVIPDYHLPTEKARAALPDRIPRKDAIYN
                     VSRATLLVSALMTGQDDLIRIGFQDMMHQPYRGDLIPGFDKVMAASGELGVLGAYLSG
                     AGSTIMCIAKEGDDQVFDNVSQFIGREFPSWEVYRHKLENEGAIVL"
BASE COUNT         2114 a         2219 c         1755 g         2469 t
ORIGIN      
        1 atcaggtcac ttggcaagga ggattgggaa gactctgatg aaatggagga gaaagattat
       61 gatcctgaac atactcttgt ttgatgattt tgaaaccctt gatgtctttg gacccgttga
      121 ggtctttgga aacctgccca aggattatga actaagatac ctttcaatgg agggcggtat
      181 tgtaaaaagt cgtcacggtg tcaaggttct aacagaagct cttgagtcct tttctcctga
      241 tgggattata tttatccctg gaggctatgg agttaggcct cttgtagggg atcctgctta
      301 tgtggaaatg gttaagggac tttgtgaaaa aagccagttt tgccttacag tctgcaccgg
      361 ctcagctctc ttggccaaaa ccggtctttt gaagggcctc ctggcaactt caaacaagct
      421 gtcctttgat tgggttgtat ccatggatcc aggtgtagat tggcaaagca tggccagatg
      481 ggcagtccat ggaaagtact ggacctcctc tggggtctct gcgggaattg atatggccct
      541 tgcctttgtt gagaaggcaa atggaagaga tgaagcagaa agggttgcaa ggtttatgga
      601 gtatatctgg aacgaggata atgacaatga cccctttgaa ataggatagg accggcaaat
      661 gccggtcctt ggcttattct attcaattga ctcctcatac agggtctgat tgtaggtctt
      721 tgtgtaactt atgtcagtta gggaaagctt tatgtcttct ccacttccat caaactccaa
      781 ggttcgggtg ttgagaatat cctcgtcctt cttctcgtag gttgattctg atgttttaag
      841 aaggtcgtaa taaactccat ctgcaaggag tctaactcca ggaatcctgg ttccctcctc
      901 tgttgttatt gtgatgtatg ttcttccatt tccattttca atggagttta tgaatatatc
      961 atttcccctt atctcaaggg tctccccctc tgctatttca agctccatat ccagcctttc
     1021 gtggacgctt acagaatcaa ggtctagtct caggtctttt gtatccttgg gaagggcatc
     1081 aaaccttaca tcgaatcttg tccccttcat gtccgtactc atacctgcac ccttcctatc
     1141 aatttcaatt ccatcagcat ataggctcat ttccatatta tgtggcatca tgtattcctc
     1201 atttaaccta tccatgccaa gggacatgag atcttggatc tcccccttca caaccgtgga
     1261 tatggctgac atgctcatgt cttcaactgt catggtcctt cctgcaagct ccaccttcct
     1321 gcccactgga actctgatgg tttcacccac agccttgttc ctatccagct taaagtctat
     1381 ttccccctct tcccacatgc catcacccct ttcattgctt agtttcaggg tcatcttcct
     1441 tacaaagaat gggggtggtt catcagttgt aaaaacccat ttctgcatat gttcttcctt
     1501 tatgatttcg ccggctccgc ttgagcctac gttcataaat ggaatgtccg gcgaaactct
     1561 gacataaaca tcgtccatgt tctttgactt ggtggtatcc tctattgtat agaagaagac
     1621 cataccgttt ccgtcaagca ttaccccgtc cacctgaacc cttatcccat cactgaaaag
     1681 ataggactta tcaataatct gtccttcacc cattctgctg agctgaagga gtgtcccatc
     1741 cataaccgta tcatacccaa tcagctgctt cccgtaaaag gcaagggttg agctgttgta
     1801 ggtaagtatt gctcccacaa gaaatattgc tgcaatcctg cttaatggga atttcctcct
     1861 tgttttttca tcatcaagtg cagccctaag cttttcctcc agtgaatcag gtgcctggag
     1921 gttgttgtat tctgttcttt tttcctcaag catcctatct agcctggtca tctaaattct
     1981 cctctcccat tgcactccta agcttgtcca tggcagtatt gacccttgat ttcactgttc
     2041 caaggggaat ctccaaaagc tccgaaatgg acttgtaatc catatctagg taatacctga
     2101 gcctaattat ttcccggtgg ctttccttga gtgtcccaag gtaataatcc aggtccatcc
     2161 tgtcctctcc ccctataaat tctatggact cctcctttac accctccacg gggataactc
     2221 tctttctggt ctccagcctg cccttgcaga ggtttaccag gatggtcttg ctccaggaat
     2281 agaaactctc ttccttccga agatttttta cattgtcata gaggcttagg atcatgtcct
     2341 gaaccatgtc ctctgcatcc tgctggttcc tcatatatac aaatgccagc ctgtaatagt
     2401 tgtccctttc ctgaagaata agcttaagaa gggcttcctt gtcacccttt ttcgccttct
     2461 tcaccagata cttcaattcc accgtctcac cccgtttcat ccctaagagt atttggagcc
     2521 caaaaaggtt catttaattt caaaaaaatt ttgcacctga aaacaggtgc aaattaatca
     2581 ttagcccaat atgtctcccg gctgtactgg tttgtcagga gatattagta caactccacc
     2641 ttgagagtag gttcccataa caaggatttc cgacatgaat ccagctatgt ttcttggtgg
     2701 aaagttcaca actgcaagga cctgcttgcc aataaggtcc tctggtgtgt agtgctctgt
     2761 tatctgggca ctggaattct tctcaccgat ttccggcccc atgtccacca ggagcttgta
     2821 tgagggcttt cttgcctttt caaaaacctc tgcctttata atttttccca cacggatatc
     2881 cagcttaaga aaatcatcaa aggtcacctg gttcttcact gtcatcctac caccttaccc
     2941 tttcgtagtt aatgtatctg tagtatatgt catcctgctg ctgtacctca gattcccaga
     3001 caacatccca atcatcatca aggtcgagat ttgggataaa ggtgtcagtg ttcctgaaat
     3061 ccttgaatat cttggtgata tatgccttgt tgcagaacag gaggagctgt ctggctattg
     3121 ttcctccacc tgttacaaaa acctccatct tcctgtcagg atttacttcc ttcagtaagg
     3181 ggaataggtc ttcaagggaa ttcacaacaa gaaacccctc gggcttatag tccttgtttc
     3241 ttgtaaccag gatgtttatc ctgtttggaa ggggcttttg gtctggtagg gcttcaaggg
     3301 tctttctccc catgattacg atgtgccctt ccgtgattct tctgaacctg tgcaggtcct
     3361 cctctatctc aaccagcatc tggccgttaa agccaatttt ccagtttcca tctactgcaa
     3421 aaatcaaatt catccttttc ctcctatgcc ttgaaattat atattggtat gatcctttca
     3481 attatctcca ctgtttcgtc caatcgctgc agaatttcct ccagtggctt gtatgcaagg
     3541 ggactttcat ccagggtgcc ttcatggacc gatgtggaat agattccctc cattgacctt
     3601 ctaaactcct caagagacgc cttctccttg gcatccctcc tgctgagaat ccgacccgct
     3661 ccatggggag ctgaaaagtt ccagtcttca tttccctttc cccttgcaag aagtgatcct
     3721 tccttcatgt tgattggaat aagcagctct tgtccctcat aggccgaaat agcacccttc
     3781 ctggtgatgt tgtccctgaa gtcaatatag ttgtggactg tgtggaagtg ctgaaaatta
     3841 tccagacttt ctccgaaaaa ctccaggagg ataagttcag ctatgccctc cctgtttatt
     3901 ttagcataat tctgggcaat tttcatatcg tgaagataat catccctttc ctttcctgtg
     3961 agatagcaaa ggtccttggg atagtccccc ttacaggatt tctgtgccag tttctggtag
     4021 tggtcggcta tctgcttgcc aaggtttctg cttcctgaat ggatcgttag gtagagggtt
     4081 cccttggagt ccctgcccaa ttctataaag tggtttcccc ctcccagggt ccccaggctc
     4141 ctttgaaacc tttccctgcc cttaaggctc ttcaggcact tcagcttttc aatttcaggg
     4201 tcagtaaacc tgttgtccct gtgggtcttt gttcctgatg ggacattttt cctgataaac
     4261 ctgtccaggt cctccagtgg aaggtctctg tcttcaagct tgaccaggag cattccacaa
     4321 ccaatgtcca cccctacaat gtttggaatc acaatatccc ccatctgggc agtaagacca
     4381 atgacacaac ccattccctg atggacatca ggcatgaccc ttaccttctt gtcctccata
     4441 tagtcctggt caagaaacct ttctatctgg tctttggctc caggctcaag gttctctgta
     4501 aaaatctcag cctctccatg ctttccacga ataatcatac tatcaccacc tgtccctatt
     4561 atctataccc aggggaagcc tcccattgtc accaaattgt aaattgaaac attcttcctc
     4621 tagtgctaga attaacataa gggaatctat caatttacct tacgaaagga gcaagctatg
     4681 aaaaaattaa ttatattgat gtttgcattg ataatggcag ttgcccttgc agcctgtgga
     4741 ggttcacagg agccggagga gccagctgag cctcctgttg tggaagagcc ggcagaggaa
     4801 cctgaagaac ctgcccagga gcctgaggaa caaactgctg aggttatact atattttgca
     4861 aacaaccagt atgttgaaac tggtgacgag caatacgaat ggatgcttac ggagacaaga
     4921 acaattgcct acgatgatga ggtctgcctg gaggaggccg tggtaagggc cctgatggag
     4981 ggacctgagg atgaggaaaa cctaagtacc ggctttccgg attcagtcca gctgattggt
     5041 gtaagcgtac ttgatggaac cgcctatgtc aacttctcaa gtgaaggcct gggaggcggg
     5101 tcaatgcagg aaagttatgt aatcagccag accgtagagt ccctgggtga acttggttct
     5161 gtacaaaggg tacagttcct ggtggacgac caggaggctg aaagcctcat gggtcacata
     5221 agtattgagg aaccaattga ggttacagtg gaataggatt aaaatcaata atccaggcaa
     5281 aggcctggat tattttcatg gtatgattat ctgtattctg ttacctgacg ggtcctttgt
     5341 gaaaagtacc ccatcctcca tttcagcctg gtagttttcg ccttgaagac tttcaaatat
     5401 tttctccaga gcttcactgt ccggtgccgc cagggtgtag tgcctgagtc cagttgcatt
     5461 ttcaggggca ggctttgcac ctcttccatt ccatacatta agtccaatat ggtggtggta
     5521 gcctcctgtt gaaataaagt tggctgatcc tgccagctgg gcgaccctct taaagcccat
     5581 caggccgtag aaccggtatg cctcctccag gtcacccacg tggaggtgca tgtgaccaag
     5641 ccttgtccct tctggcatac ctgtccatgg tgtatctctc ccaagctcca taagccccct
     5701 gaaatcaagg gggtcagtac ccatctggac ctggtctcca tcccactccc atttgctgtc
     5761 gggaatatca gcatagacct caatgccgtt gtcctctgga tcctggaggt atagggcctc
     5821 gcttacctca tggtctgcac cgcctgtcac cggaacattt gcatcccgca ggtggtttat
     5881 aaagctcccc aaatgttttc tagtgggaag tagaattgcg aagtgataga gtcccgtcct
     5941 tcttggaggt ttctgaatca cattctcagg cctcaccaag gtcaagatcc ttcttccatc
     6001 cactcccagg tcaacaaagt catttttctt ttcaagtatt tccaacccca agctgtcctt
     6061 ataaaatctg gtcatcaggt ccatatccat tacattcaga cttagattaa gcggatatat
     6121 cagtgaaccc tcgtgaaatt tgttcatatt tatcaccttc tatctattag tggtctacat
     6181 ttacccaggc aaggaaatcc taaaccatta accctattcc caccaggtcc tgtataccac
     6241 atagggtatt agggcaaata cgaatacaaa ttcccttgga gcgtaaacga agtcacccgt
     6301 aagccagaaa agcactgcta tcctgtcagg aagaaccagg tatattaggc ccaggaccac
     6361 cagggaaaca atcaacacca ctatcccttt cctgtttttc accctctccc tcctttgtcc
     6421 ctaatgtttc aatatgctta aaagcctctt catatacctt atacccaccg ctcccagtgg
     6481 agccgtcaca acaattgaaa gaaccgccat tgaaaggatc aggtctcccc cctggactcc
     6541 ataggcaagg ggtaccgctc caatggctgc ctggactgtg gcctttggtg tatacgcaac
     6601 tgcgcagaag gccttttcct tcctgttaag gtctgtaccc aaaagagcaa tgtagacccc
     6661 aagagacctc atcaggaggc cgactgcaat tattaggata cctgcacctc cagcatccag
     6721 ggtcactccc aggtccacct gggctccaac aagaacaaac agtagtattt ccgcaagtat
     6781 ccaaatcttg ttgaacttga ctgagagcct ttttgcgact attccccgct ttaaaagtat
     6841 tacaaagcct attgtcatca caccaagaaa accggaaaag gctatggtgt ccctgataag
     6901 gtcctcaaga cccataagga gtattgccgc tccaagtatc ataagaacct tctttgtatc
     6961 cctaatgtgt tgggtctcaa aaatccttac aaatacatga cccatcaggt agcctaatcc
     7021 cacccctgta attatcgaaa ttggtattcc catcaactga cctgccagac taacattttc
     7081 tcccccatag agtcccaaaa aacttgtaaa tatggttatt gcaacaatat catcaaggga
     7141 tgctcccgcc aggatcaggg tagggatgtt cttgtcattt ccccttccct catcattcag
     7201 ctggagcatg gcaggtacaa ccactgccgg ggaaacggca gcgattataa agccaaggat
     7261 tccaccctga accatggaaa aaccgaagaa aacagttgat acgaccatta cagtcaagcc
     7321 ttcaagtaca cctggaataa agctcaagcc cagggcaggc cttcccacct ttttcagcac
     7381 ctcccagtcc aggcctaggc ctgcccgaag gagtattatt ataagtgcta tttcccgaaa
     7441 gtcagccgat accctcatca ggtcagggct aatgaaatca aagccaccag ggcccagcaa
     7501 cacccccaca agcagcatgc ccaagagacc cggtatcttc accttctcca tcaggtagtt
     7561 ggctgtcaaa cccaatattg ttattattcc caaactagtc gccatttcat cctccaattt
     7621 ttagacgtca aaaaactcct gccgggaact catctaaagt tcctggcagg agtcattagc
     7681 cgaagcagtt aaaggtgaac cccatcacct atttacattc actgtataat tataacctct
     7741 gtattccctt atggtcaatg gttatagtac aattgcacct tcattttcaa gcttgtgcct
     7801 gtaaacctcc cagcttggga actccctacc tatgaactgg gagacattgt caaacacctg
     7861 gtcatcccct tccttggcga tgcacatgat tgtagaacct gctccgctta ggtatgcacc
     7921 caggaccccc aattcacctg acgcagccat taccttgtca aagccaggaa taaggtctcc
     7981 cctgtagggc tggtgcatca tgtcctggaa tcctatcctg atcaagtcat cctgtcctgt
     8041 cataagggca gatacaagca gggtcgcacg acttacattg taaatggcat ccttgcgggg
     8101 aatcctgtcc ggcagggcag cccttgcctt ttcagtggga aggtggtaat cagggataac
     8161 ggttataaag tcataagtgt ttttcagagg aatcctgata ctgtacacct ctccctcctc
     8221 catggctgat atgacaaccc ctcccaaaag ggcaggtgta acgttgtccg ggtgaccttc
     8281 catgtaggtt gcaatcctta ggatatcctt tctctccagg ggacgtccca acacctcgtt
     8341 tgcccccatc attcccccga ttatacagga tgatgatgat cccaggcctc ttgcgattgg
     8401 gatttccgca tctattgtaa tctcaaccgg ttccggatcc cttccaagat gctcaaatac
     8461 catgttgtag ccttggtgaa ccattgaact gatctttgtg gacccggcat tgggtttaaa
     8521 tgaaaactca ttgtacctgt ctattgcaag acccatg
//
LOCUS       DASRQF010000064         6189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1327002, whole genome shotgun sequence.
ACCESSION   DASRQF010000064 DASRQF010000000
VERSION     DASRQF010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6189
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327002"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="VFD79_01655"
     CDS             complement(<1..142)
                     /locus_tag="VFD79_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HZH92782.1"
                     /translation="MIKVGIITSSDKGSAGQREDLSGAVIKEMVEDKGYKVIRQVILP
                     DEQ"
     gene            complement(145..639)
                     /locus_tag="VFD79_01660"
     CDS             complement(145..639)
                     /locus_tag="VFD79_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014969036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HZH92783.1"
                     /translation="MKYEQVIRYAMQMELDGSNFFKEKADKFSNATTRKLFLTLAETE
                     MEHYEYLKKQLDSYMENETFDTSDQVMDREEDIFKAREESEHIEATLRESDIPDLTIL
                     RMAYLIERDYKEFYENAAQSADDDGIKQIFQKLSKWEEGHERLFKSEYDRRMKEYMTL
                     PWGG"
     gene            complement(651..3320)
                     /gene="fdhF"
                     /locus_tag="VFD79_01665"
     CDS             complement(651..3320)
                     /gene="fdhF"
                     /locus_tag="VFD79_01665"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(2277..2279),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HZH92784.1"
                     /translation="MQVTINGKQYEFEKGTTLLKACNEIGIDIPALCNDERLVPSGSC
                     RLCQVEVEGWGKLHTACTTQVRDGMVVHTHSEKVVKARQDILDMLISNHPLECLTCEK
                     SGACKLQDYCYEYGITEGTYRWEMSKPYFDDTNPFYTFDARKCILCGLCVRVCDELQG
                     TNAIGFENRGFDTQIATPFGDGLENSTCVSCGNCVSVCPVGALEPKSKKKFRHWETRT
                     VRTTCSYCGVGCQMDMKVKGNEIVEIQPAFDAHNEGLLCVKGKFSYNFLSHPDRLKMP
                     MVRKDGVLVEASWDEALEVITSKMKEVKEDFGSDAFAGLTSARCTNEDNYMFQKLFRS
                     TLGTNNVDHCARLUHASTVAGLAITLGSGAMTNSIQEIKNADTIFIMGSNTSENHPVI
                     GAIMKQAKKAGAKIIVADPRRIEMAEYSDVFLQLKPGTNIALLNGLMHVIIEKGLQDE
                     AYIRERTENYEELKELVKGYTPERVAEICGLDPEDIIKAAEIYGEAEKAGIYYTMGVT
                     QHTTGTHAVMSISNLALLCGNVGVESAGVNPLRGQNNVQGACDMGGLPADLPGYQKVF
                     KPEVLDKFQESWGSKLSNKVGLTITEMFKAIEEDQVKFLYIMGENPMVSDPDTNHVDH
                     VLDHVDFLVVQDIFLTETAAKADVVLPAAAFAEKDGTFTNTERRIQRVRKAIEAPGEA
                     KADWVILMDIMNRMGDETKYSHPARIMAEIASLTPSYRGVSYKRLEKESLQWPVLDEN
                     HPGTKYLHKTAIARGRGLFMPAEHVASAELTSDEYPLILTTGRILYHYHTMTMTGRED
                     GLNKLAPHSYVEINETTANKLGIEDGDMVRIYSRRGELQTAALVTDIIDDGVVFMPFH
                     YTEGKANILTNSAIDEIAKIPELKVAAVRIEKA"
     gene            complement(3321..4277)
                     /locus_tag="VFD79_01670"
     CDS             complement(3321..4277)
                     /locus_tag="VFD79_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014969039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZH92785.1"
                     /translation="MSTVRLTIDGKTAEVSNQLTVLQAAKTVGIDIPALCYDETLEIF
                     GACRMCIVEIEGSRKLQTACSTLVKDGMVVHTETERVVWNRKQTLQMLLDSHPNDCLT
                     CHKAGECLLQDYAYRYDVSFRKHDGEMRPQLLDTTSPYILRDNSKCILCGKCVMTCAQ
                     VSDRQVLSFAERGYDTRIIFGADESIEESPCVSCNRCVAVCPVGALVDKRDMGKIRSW
                     EAETKIVGCKACEYGCEFEVRSRDRKPVSVVAKAPTNGRPLCLKGRLTTELKYLDNPD
                     APYRKLDKSFEKTTWDKALQITELLEKIARIEGSNNNGEGEE"
     gene            complement(4293..5873)
                     /locus_tag="VFD79_01675"
     CDS             complement(4293..5873)
                     /locus_tag="VFD79_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004838147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HZH92786.1"
                     /translation="MITKEKLFEIKEKTKPSLVEKLLESDRVEFRGEDLVLKGEYYKK
                     QVRIALKNCDIIDPTNIEEYIALDGYFALFKALTELEPGDVVDEITESLLRGRGGAGF
                     PTGRKWNEAYKYQADKKYIICNADEGDPGAFMDRSILEKDPHSVLEGMALGGYAIGSD
                     QGYIYVRAEYPMAVKRLQIAIDQAREYGLLGKNIFGTGFNFDIELRLGSGAFVCGEGT
                     ALMESIEGRRGMPRPKIHRTAHKGLWGKPTIINNVETLANVPVIMNRGAKWFRGIGTE
                     ESPGTKVFALVGKVENAGLVEVPMGMTLREIVFDIGGGAQDGKEYKAVQTGGPSGGCI
                     PAELIDTPVDFESLGKIWSIMGSGGMVVMDENTCMVDIAKFFLDFSVEESCGKCVPCR
                     EGTKRMYEILDRITKGEGVEGDIERLEELSETVSSTSLCGLGQTAPNPVITTIKYYRH
                     EYEAHIKDKSCPAKSCKALIKYEITEGCIGCTRCAKVCPVVAITGKVKEMHHIDQSIC
                     IKCGACYDVCPVDAISII"
     gene            complement(5886..>6189)
                     /locus_tag="VFD79_01680"
     CDS             complement(5886..>6189)
                     /locus_tag="VFD79_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009372536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HZH92787.1"
                     /translation="AEIYGVATFYSQFSFIPTGKNKISVCLGTACYVKGAQSILEEIE
                     TQLGIKSGETTKDEVFSIASARCVGDCSIAPVILVNEDVYAKVKKDDIAEILAKYN"
BASE COUNT         1354 a         1673 c         1229 g         1933 t
ORIGIN      
        1 cctgttcatc aggcaggatt acctgcctga tgaccttata gcccttgtct tcaaccattt
       61 ccttgatgac cgcaccggaa aggtcctccc tttgtccggc tgagcccttg tcacttgatg
      121 taattatacc aaccttaatc atgtctaacc tccccaagga agagtcatgt attccttcat
      181 ccttctatca tattcagact tgaacagcct ttcgtgacct tcctcccact tggaaagctt
      241 ctggaaaatc tgttttatgc catcgtcatc agcgctctgg gctgcattct cgtagaactc
      301 cttgtagtcc ctttcaatga ggtaggccat cctaagaatt gtcaggtctg ggatgtcgga
      361 ttcccggagg gttgcctcta tgtgctcgga ttcctcccta gccttgaaga tgtcctcctc
      421 acgatccatc acctggtcac ttgtatcaaa ggtctcattt tccatatagc tgtccagctg
      481 cttcttaagg tattcataat gctccatctc ggtttcagca agggtaagga acagcttcct
      541 ggttgtggcg tttgaaaact tgtccgcctt ttctttgaag aaattgctcc cgtcaagctc
      601 catctgcatt gcatatctta ttacctgttc atatttcatc ctattcacct ctatgctttc
      661 tctatcctta ctgcagctac cttcagttca ggaatcttgg ctatttcgtc tattgcacta
      721 tttgtaagga tgttggcctt tccttcagtg tagtggaagg gcataaacac aaccccgtcg
      781 tcgataatat cagttacaag ggcagctgtc tggagctcac cacgtcttga atagatcctg
      841 accatgtccc cgtcttcaat tcccagcttg ttggctgtgg tctcatttat ctccacgtat
      901 gaatgtggtg ccagcttgtt gagcccatcc tccctacctg tcattgtcat tgtatggtag
      961 tggtagagta tccttccagt ggtaaggatc aatgggtact catcacttgt aagctcggcg
     1021 ctggctacgt gctcagccgg catgaagagt cctctgcccc tggcgattgc agtcttgtgg
     1081 agatactttg taccagggtg gttctcatcg agaactggcc attgaaggct ttccttctca
     1141 agtctcttgt atgagacccc tctgtaggat ggagtcaggg atgcaatctc tgccattatc
     1201 ctggccggat gggaatattt ggtctcatcc cccattctgt tcatgatatc catcaggatc
     1261 acccaatcgg cctttgcctc accaggtgct tcaattgctt ttctgaccct ttggattctt
     1321 ctttctgtgt ttgtaaaggt tccgtccttc tcggcaaagg ctgctgccgg caggacaaca
     1381 tctgccttgg ctgcagtttc tgtaaggaaa atgtcctgta caaccaggaa gtccacatgg
     1441 tcaagtacat ggtcgacatg gttggtatca gggtctgaaa ccattgggtt ttcccccatt
     1501 atatagagga atttaacctg gtcttcctca attgccttaa acatttccgt tattgtaagt
     1561 ccaaccttgt ttgaaagctt gcttccccag ctttcctgga acttgtcaag gacctcgggc
     1621 ttgaaaacct tctggtagcc tggaagatct gcaggtaggc cacccatgtc gcaggcaccc
     1681 tgaacgttgt tctgccccct cagtggattg actcccgctg attcaactcc aacatttccg
     1741 caaagaagag ccaggtttga aatgctcatt acagcgtggg tacctgttgt gtgctgggta
     1801 actcccatgg tgtagtagat tccggctttt tctgcttctc cgtagatttc tgctgccttt
     1861 atgatatcct cgggatccag accacagatt tcagcaaccc tttcaggtgt atagccctta
     1921 acaagctctt tcagctcctc gtaattctcg gttctttccc tgatgtaggc ctcatcctga
     1981 agtcccttct caatgattac atgcatcaag ccattcaaga gggcgatgtt tgttcccggc
     2041 ttaagctgca ggaatacatc tgagtactcc gccatctcaa tccttcttgg atctgccacg
     2101 ataatctttg ccccggcctt tttggcctgc ttcataattg ctccaattac cgggtggttc
     2161 tctgatgtgt ttgaacccat gataaagatt gtatctgcat tcttgatttc ctgtatactg
     2221 tttgtcatag ctccgcttcc caatgtgatt gcaagacctg caaccgttga agcgtgtcag
     2281 aggcgggcgc agtggtcaac gttattggtt cccagggttg acctgaacag cttctggaac
     2341 atgtagttat cttcatttgt acaccttgcc gaagtaagac cggcaaatgc atctgaacca
     2401 aagtcctcct tcacctcttt catctttgag gtgattacct ccagtgcctc atcccagctt
     2461 gcttccacca ggactccatc ctttcttacc attggcattt tcaacctgtc cggatgggat
     2521 aggaagttat atgaaaactt accctttaca caaaggagtc cttcattatg ggcatcaaat
     2581 gcaggctgga tttccacaat ttcatttccc tttaccttca tgtccatctg acagccaact
     2641 ccgcagtagg agcaggtagt tctaacagtc ctggtctccc aatgtctgaa cttcttcttg
     2701 ctctttggct ccaatgcccc cactggacat actgatacgc agtttccaca ggatacacag
     2761 gttgagttct caagtccatc tccaaaggga gttgctattt gagtatcaaa tcccctgttc
     2821 tcaaagccta tggcatttgt tccctgaagc tcgtcgcata ccctgacgca caagccgcag
     2881 aggatgcatt ttctggcatc aaatgtatag aaagggtttg tatcgtcaaa atatggcttg
     2941 ctcatttccc atctataggt tccttcagta attccatact cgtagcagta atcctgaagc
     3001 ttgcaggcac ctgacttttc acaggtcagg cactcaagtg ggtggttgga tataagcata
     3061 tccaggatgt cctgtctggc ctttaccacc ttttcgctat gggtatgtac taccattcca
     3121 tccctcacct gggtggtgca ggctgtgtgc agcttacccc agccctctac ctccacctga
     3181 caaagtctgc aggatccaga gggtacaagt ctttcatcat tgcacaaggc ggggatgtct
     3241 attcctattt cattgcaggc ttttaggagt gttgtacctt tttcaaattc atattgtttg
     3301 ccgtttattg taacctgcat ctactcttcc ccctctccat tgttgtttga cccttcaatc
     3361 cttgctattt tctccagtag ctcagtgatt tgaagagcct tgtcccaggt cgtcttctca
     3421 aatgacttgt ccagctttct atatggtgca tccggattat ccagatactt aagttcagta
     3481 gtgagtcttc cctttaggca caggggtctt ccattagtcg gtgccttggc aaccactgaa
     3541 acaggctttc tatccctgga tcttacttca aattcacagc cgtattcaca ggccttgcag
     3601 ccgacgattt tggtttctgc ctcccaagac ctgattttgc ccatgtccct cttatcaacc
     3661 agggccccta cagggcatac tgcaacacac ctgttgcagg agacacaggg agattcctct
     3721 attgattcat ctgctccaaa tatgattctt gtatcatagc ctctttccgc aaaggaaaga
     3781 acctgtctgt cagatacctg tgcacaggtc atgacgcatt taccacagag gatacactta
     3841 ctgttgtctc tcaatatgta tggacttgtg gtatccagaa gctggggcct catttcccca
     3901 tcatgctttc taaagcttac atcatatctg tatgcatagt cctgcagtag acattcccct
     3961 gccttgtggc aggtcaagca atcatttgga tggctgtcca aaagcatctg aagtgtctgc
     4021 tttctgttcc ataccaccct ttcagtttca gtatggacaa ccattccatc cttaacaagg
     4081 gtggaacatg ctgtctgaag ctttctgctt ccttctatct ctacaatgca catcctacat
     4141 gccccaaata tttccagggt ttcatcatag caaagggcag gaatatcaat ccccacagtt
     4201 ttcgccgcct gcaggacggt cagctgattg gatacctcag ctgtcttacc atctattgtt
     4261 agcctcactg tactcatatt cttcctccaa tgctatatta ttgaaattgc atcaacagga
     4321 cacacgtcat agcaagctcc acattttatg catatagact ggtctatgtg gtgcatttcc
     4381 ttgacctttc ctgtgattgc aaccaccgga catacctttg cacatctggt gcagccgatg
     4441 catccttcag ttatctcgta ttttataagt gccttgcagc tcttggctgg gcaagacttg
     4501 tcctttatgt gcgcctcgta ttcatgtctg tagtatttta ttgttgtaat tacagggttt
     4561 ggagcagttt gtccaagccc gcaaagggat gttgagctta cggtttcaga gagctcctca
     4621 agtctttcta tgtcgccttc aactccctca cccttggtta tcctgtccag tatctcatac
     4681 atcctcttgg ttccttccct gcatggaaca cacttaccgc aggattcttc tacgctgaag
     4741 tccaggaaga acttggcgat atccaccata caggtgtttt catccattac taccattcca
     4801 cctgatccca tgatggacca gatcttgccc agggactcaa agtccacagg ggtatcaatc
     4861 aactcggcag gtatgcagcc tcctgaagga ccgcctgtct ggacagcctt gtattccttt
     4921 ccgtcctggg ctcctccacc gatgtcaaat acgatctccc taagggtcat tcccatggga
     4981 acctcaacaa gaccagcatt ctcaacctta ccaacaagtg caaaaacctt ggttccaggt
     5041 gattcctcag ttcctattcc cctaaaccat tttgctcccc tgttcatgat taccggaaca
     5101 ttggccagtg tctccacatt attgattatt gtaggcttac cccataggcc cttgtgtgct
     5161 gtcctgtgga tctttggacg aggcattccc ctcctgccct caattgactc catcagtgct
     5221 gtaccttctc cgcatacaaa ggcacctgaa ccaagtctaa gctcaatatc aaaattaaat
     5281 cctgttccga atatgttctt gcccaatagt ccgtactccc tggcctggtc tattgctatc
     5341 tgcagtctct taactgccat tgggtactca gcccttacat atatgtagcc ttggtccgaa
     5401 ccaattgcat atcctccaag ggccatgccc tcaagtacgc tatgaggatc cttctcaaga
     5461 attgacctgt ccatgaaggc acctgggtct ccctcatcag cgttgcatat tatgtatttc
     5521 ttgtcagcct gatatttgta ggcctcattc cactttcttc ccgtggggaa tcccgctcct
     5581 cctctccccc gtagcaggga ttctgtgatt tcatccacca catctccagg ttcaagctct
     5641 gtcagggcct tgaatagagc gaagtatccg tcaagcgcta tgtactcttc tatatttgtt
     5701 ggatctatta tgtcgcagtt cttaagggca attcttacct gcttcttgta atactctccc
     5761 tttagtacaa gatcctctcc cctgaattca accctgtcac tctcaaggag tttttctacc
     5821 agggatggct tggttttctc ctttatttca aacagcttct cctttgttat catggcactc
     5881 tccccttaat tgtatttagc aagtatctct gcaatgtcgt ccttcttgac ctttgcatat
     5941 acgtcttcat tcaccagtat aacaggtgct atactgcagt ctccaacaca tctggctgat
     6001 gcgattgaaa aaacctcatc ctttgttgtc tcaccggatt tgattcccag ctgggtctcg
     6061 atttcctcaa gtatactctg ggcacccttt acatagcagg cagttccaag gcagacactg
     6121 atcttgttct ttcctgtagg tataaatgaa aactgtgaat aaaaggttgc cactccataa
     6181 atctctgcc
//
LOCUS       DASRQF010000065         6584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1697022, whole genome shotgun sequence.
ACCESSION   DASRQF010000065 DASRQF010000000
VERSION     DASRQF010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1697022"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VFD79_01685"
     CDS             <1..460
                     /locus_tag="VFD79_01685"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZH92788.1"
                     /translation="MTQFDGLTGLYNAATAKVMIEENIKNKTHGRIDILVLIDLDNFK
                     DVNDNYGHLMGDKTLKNLGKILRRTFRNNDIIGRIGGDEFCVYLKDVSSIESIEHKCD
                     QVNDSLMDGSYEIKTSVSFGLSIVEDGDDYEKVFIKADKEMYHNKGLKQR"
     gene            597..1880
                     /gene="kynU"
                     /locus_tag="VFD79_01690"
     CDS             597..1880
                     /gene="kynU"
                     /locus_tag="VFD79_01690"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HZH92789.1"
                     /translation="MKYEFKDGLEFAKAMDEKDVLKGFRARFYIHEDEIYMDGNSLGL
                     ASRDAEDSLQNMLRVWKEEAIRIWGAEDGRYFNYSKVLAEKAKDLINADAEEIIFMGS
                     ITTNIHQALATFYKPTEDRYKIIVDELNFPSDIYATKSIIELKGYSIEDALVRIGTRD
                     GRTILEEDIIAEMKDDVALILLPSVLYRSSQLLDMERITREAHDRNILVGWDLAHSMG
                     SVPHDFKKIDPDFAVWCTYKYLNGGPGATAGMFINKRHFGESSGLKGWFGSKDESQFL
                     MSHDFDQDRDANGWLLGTHSLFSMAPIEGSLDMFNEAGMDNIRKKSLHITAYLMYLID
                     SKLARYGYSVGNPRENSRRGGHVALEHEEAYRISLALRDNKVIPDYREPNVIRLAPIA
                     LYNTYQEVYRLVEILEEIVVSKAYEKYTLDRVTVL"
     gene            1987..2748
                     /locus_tag="VFD79_01695"
     CDS             1987..2748
                     /locus_tag="VFD79_01695"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HZH92790.1"
                     /translation="MMVKQMQLEIIAEENDKYSIRISDDMDQVIITYNTQTKDLQIPG
                     GNELADFLVSNESQFRGILHNKKSESYYKGFKLDVSIIDGKDISKFKNRDNIIIRDRG
                     KVFVEPACKSSNLPELYTDASYDERLNKGAYCVLRKEPDGSYEAREFLCESQDSNSAE
                     LEAVIKALEIYEGDLRVVTDSQYVRKGITEWIIHWKLNNWHTANGTKAKNISMWKKLD
                     LLCENRRIEFGYVKGHNNHFENEYCDLMARIKRES"
     gene            complement(2762..4498)
                     /locus_tag="VFD79_01700"
     CDS             complement(2762..4498)
                     /locus_tag="VFD79_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZH92791.1"
                     /translation="MIKVNELTYFFPQKDLYDKVSFELTDGQHCAFIGSSGIGKSTLI
                     NIIMDPESYMFDGSLEIDPLCRIGFVSQFSQLDKSDELTVFEYIGENYLKLQSRIESI
                     FSEMESSGDIEALLEEYQVLLDSYEAIGGDGFESLIDRKLNLANLHGLRDKLVTDLSG
                     GEFKLIQVIKEMLAKPNLLIMDEPDAFLDYGNLNSLKNLINSHKGILLVITHNRYLLN
                     HCFNKVLHLENKEIQEFNGRYIEYNLELLKTKIEMQELAAIDTAEIERNNVLIEKLRF
                     EANQNAEAAKGRSVNARVKIQERLKARRIKPPFIAIKQPDIRFSTDREIEDRVILTLR
                     DFQAAFEDVILENVSFEVNSRDKVALIGSNGTGKTTLLREIYKNSNEAIELDGDVKVE
                     YLSQLQGEMLDEESTIADEFFNSGFKSYDEIKLCLSSYGFDEGIIDQRISSLSGGEKN
                     MLQLAKISSSNADFLLLDEPTSHLDTYSQIALEKALKEFDGSVLMISHDYQTIINSMD
                     YILIIEDKSIRRMSVRKFRKMIYDAYFDKNYLEIEQKIKSIETSIELALKDNDYERAK
                     ILSEDLEKVIGL"
     gene            complement(4701..4901)
                     /locus_tag="VFD79_01705"
     CDS             complement(4701..4901)
                     /locus_tag="VFD79_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HZH92792.1"
                     /translation="MSVVKVVEILAESNEGWEAAAQKAVEEVSKTVKNVTSVYVKDFQ
                     AIVEDGKIVKYRVNVKVSFIVE"
     gene            5087..5551
                     /locus_tag="VFD79_01710"
     CDS             5087..5551
                     /locus_tag="VFD79_01710"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HZH92793.1"
                     /translation="MLEYEKKLKDYIANNNIDADHLHFDKICHSVQEAADAAGATPDD
                     LVKNICMVDDKGNLIVAIVKGEDRVSTSRVGKVLKIERPRTATEEEILEKTGFPCGGV
                     PSFGYHATFIVDPRVMEKEYVYSGGGSEYSLVRMSSQELTRVNSGIITRVRK"
     gene            5556..5753
                     /locus_tag="VFD79_01715"
     CDS             5556..5753
                     /locus_tag="VFD79_01715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HZH92794.1"
                     /translation="MEGTTRNSIMLGTKVSIVQKQDQRSGALTQGVVAKILTKSQTHP
                     HGIKVMLQDGTVGRVKEILED"
     gene            5766..6104
                     /locus_tag="VFD79_01720"
     CDS             5766..6104
                     /locus_tag="VFD79_01720"
                     /inference="COORDINATES: protein motif:HMM:NF015893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HZH92795.1"
                     /translation="MNIQIYGANKCFDTKKAERYFKERKIKFQYKDLYRYGLSKGEFD
                     NIKAAVGMESLINTKSKDYEKLNLHKIRGNAIREEIILENPKLFNTPIVRNGKQATVG
                     YKPEIWEDWE"
     gene            6141..>6584
                     /locus_tag="VFD79_01725"
     CDS             6141..>6584
                     /locus_tag="VFD79_01725"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HZH92796.1"
                     /translation="MKLTYLGHSSFLVEEGKFKGVVDPFLSGNQASTTRPDDIKDLTH
                     VFITHGHGDHIGDAVQLAKANDALVITNAEIADYLSSKGLRVHAMHIGGRFRFDFGTV
                     KMTPALHGSGIKTDKGMLYGGNPGGFVIEVNGKKVYHAGDTGLTMD"
BASE COUNT         2033 a         1243 c         1479 g         1829 t
ORIGIN      
        1 aatgacacag tttgatggct tgactggttt gtacaatgca gcgacagcaa aggtaatgat
       61 tgaggagaac atcaagaaca agactcatgg aagaattgat atacttgttt tgatagactt
      121 ggataacttc aaagatgtaa acgacaatta cggacatctt atgggggata agaccttgaa
      181 aaacctaggc aaaatactta ggagaacatt caggaacaat gacataatag gtcgaatagg
      241 tggagatgag ttctgtgtat atcttaagga tgtttcatca attgaatcaa tagaacataa
      301 gtgtgaccag gtaaatgata gcctgatgga tggaagctat gagattaaaa catcagtaag
      361 ctttgggttg tcaatcgttg aagatgggga tgactatgag aaagtattta ttaaggctga
      421 taaggagatg tatcataata aaggattgaa gcaaagatag cttagatcta caaaacgaag
      481 atccatgtca gataatcatc aattatgaaa cctgatgggt ctttcttgta cacattatca
      541 tatgattttt tacccatgaa tgctacaatt gtatcaagaa caaagggggc gatagcatga
      601 agtatgaatt caaggatgga ctggaatttg caaaggctat ggatgaaaag gatgttctta
      661 aaggtttcag ggcgagattc tacatccatg aggatgagat ctacatggat ggtaattcat
      721 taggccttgc ttcaagggat gcagaggatt cactccaaaa tatgttaagg gtatggaaag
      781 aggaggcaat caggatatgg ggtgctgaag acgggagata cttcaactat tccaaggtac
      841 ttgcagaaaa ggcaaaggac ctgataaatg ccgacgctga agagattata ttcatgggga
      901 gcataaccac aaatatccat caggcgcttg caaccttcta caagccaaca gaggacaggt
      961 acaagatcat agtggatgag ctgaattttc cctcggatat ctatgcgaca aagagcataa
     1021 ttgagcttaa gggatactct attgaggatg cacttgtaag gatagggacc agggatggac
     1081 ggaccattct tgaagaggat ataattgctg aaatgaagga tgatgtggct ctgattcttt
     1141 tgccatctgt actctacaga agctcccagc tgcttgatat ggaaaggata acacgggaag
     1201 cccatgacag gaacatcctg gttggttggg accttgccca ttccatggga tccgtaccac
     1261 atgacttcaa gaagattgat ccagactttg ccgtatggtg cacctacaag tatctgaacg
     1321 gtggaccggg agcaacagca gggatgttca tcaataaaag acactttgga gaatcctcag
     1381 gactcaaggg ttggtttggc agcaaggatg agagccagtt ccttatgagt catgattttg
     1441 accaggatag ggatgcaaac gggtggttat tgggaacaca cagcctattc tcaatggctc
     1501 caatagaagg gtccttggac atgttcaacg aagctggaat ggataatatc aggaagaaat
     1561 cattgcatat cacagcctat ctcatgtatc tgattgacag caagcttgcc aggtacggat
     1621 attcagttgg taatccaagg gagaattccc gaaggggagg tcatgttgcc cttgagcatg
     1681 aggaggcata tagaattagc ctggccttaa gggataacaa ggtaatacct gactacaggg
     1741 aacccaatgt aatcagactg gcacctatag ccctgtacaa tacctaccag gaggtttata
     1801 gactggttga aattctggag gaaattgtgg tttcaaaggc ttatgagaaa tacaccctgg
     1861 atagggttac tgtactttag aaagcaagaa tcaaaaacct gttgggagta tttccaatgg
     1921 gttttttttg atggaataag ggcatatgtt ctgttacaat tgaaaataag aaaggaggtc
     1981 atagtcatga tggtcaagca gatgcagctg gagatcatag ctgaagagaa tgataagtac
     2041 tcaatacgaa tttcagatga tatggaccag gttataatta cctataatac tcagaccaag
     2101 gaccttcaaa tccccggagg aaatgagctt gcagattttc ttgtaagtaa tgaaagtcag
     2161 tttagaggga ttctacataa caagaaaagt gaaagctatt acaagggatt caagctggat
     2221 gtttccataa tagatggtaa ggacatctcc aagtttaaga accgggataa tatcatcata
     2281 agggataggg gcaaggtctt tgtagaacct gcttgtaaga gtagcaacct gcctgagtta
     2341 tacactgatg ccagctatga tgagaggcta aacaagggag catactgtgt ccttagaaag
     2401 gagcccgacg gttcatatga agccagggaa tttctatgtg agagccagga ttccaattct
     2461 gctgagcttg aggctgtgat aaaggcacta gaaatctatg agggtgactt gagagtcgtg
     2521 acagactctc agtatgtcag aaaggggatc accgaatgga tcatccattg gaagcttaat
     2581 aattggcaca cagctaacgg gaccaaggcc aagaacatta gcatgtggaa aaagcttgat
     2641 ctgctttgtg agaatagaag aatcgagttt ggatatgtaa agggccacaa taatcatttt
     2701 gaaaatgagt attgtgacct tatggcaaga atcaagaggg agagctgaca agagcttata
     2761 tctataagcc tataaccttc tcaaggtcct ctgagagtat ctttgccctc tcatagtcat
     2821 tatccttaag ggccaactcg atacttgttt caatggactt tattttctgc tcaatctcca
     2881 aatagttttt atcaaaatag gcatcgtata tcatcttcct aaacttccgt acgctcattc
     2941 ttctgatgga cttatcttca ataatcagga tatagtccat ggaatttatg attgtctggt
     3001 agtcgtgtga aatcataagc actgaaccat cgaattcttt caaagctttt tctagagcta
     3061 tctgcgagta tgtgtccaga tggcttgtag gctcatccag tagaaggaag tcagcattgc
     3121 ttgaagatat ctttgcaagt tgaagcatgt tcttttctcc accggatagt gaacttatcc
     3181 tttgatcgat gatcccttca tcaaatccgt agctggaaag gcaaagcttg atttcatcat
     3241 aggacttgaa gccagagttg aagaattcgt cggctattgt gctttcctca tccagcatct
     3301 ctccctgaag ctgggacagg tactccacct ttacatcccc atcaagttca attgcttcgt
     3361 tgctattctt gtaaatttct ctgagcaggg ttgtttttcc ggtgccattc gatccgatta
     3421 gtgccacctt gtcccttgag ttgacctcaa agctgacatt ctcaaggatt acatcctcga
     3481 aggcagcctg gaagtctcta agggttagaa taaccctgtc ttctatttcc ctatctgtgc
     3541 tgaaccttat gtcaggctgc ttaatggcta taaatggggg cttgattctg cgagccttca
     3601 gtctctcctg gattttaacc cttgcattta cggacctacc cttggcagct tcagcattct
     3661 gatttgcctc aaatctcaat ttctctatta gaacattgtt tctctcaatt tctgcagtgt
     3721 caattgctgc cagctcctgc atttcaatct ttgtttttag caactccaag ttatactcaa
     3781 tatatctacc attaaattcc tggatttcct tgttttcaag atgcaggacc ttattgaagc
     3841 aatggttaag gagatacctg ttgtgtgtta ttacaagaag gattccctta tgggagttta
     3901 taagattttt aagtgaattg aggttcccat aatcaagaaa tgcatctggt tcatccataa
     3961 tcaacaggtt aggcttggca agcatttcct taatgacctg tataagcttg aattcaccac
     4021 cacttagatc agtcaccagc ttgtccctta aaccatgcag gttggcaaga ttgagctttc
     4081 tgtcgataag gctttcaaaa ccatctccac caatggcttc gtaagaatcc aaaagcacct
     4141 ggtattcctc aagcagggct tcaatatctc ctgagctttc catttcagaa aagatagatt
     4201 ctatcctgct ttgcaacttc agataattct ctccaatata ttcaaacact gtcagttcat
     4261 ctgatttatc cagctgagaa aactggctta caaatccaat cctgcaaagt gggtcaatct
     4321 ccaaggaacc atcaaacata tagctctccg gatccataat tatatttatc aacgtactct
     4381 tcccaatccc acttgaacca ataaaggcac agtgctggcc gtctgtcagc tcaaatgaaa
     4441 ccttgtcgta taggtctttc tgtgggaaga agtaggttaa ctcattaact tttatcatat
     4501 taatacctct ctctaaaaat gaaagagctt aaacaataag ctcctagcaa ataattcaac
     4561 ttagtttttg ccacgtaatt taggcaacat attttcttta ccgacgtagc ataacatttt
     4621 tttggtctaa tatcaatcat ttcatgaaaa aacaaagacc acggaggcct gttatgcttc
     4681 catggtccaa tttctttagt ctactccacg atgaatgaaa ccttcacatt gactctgtac
     4741 ttgactatct tgccatcctc aacaatagcc tgaaagtcct taacataaac tgatgtaaca
     4801 ttttttactg tctttgatac ttcctcaact gccttctgag ctgctgcttc ccaaccttca
     4861 ttggattccg ctaaaatttc aacaacctta accactgaca ttttccaaat cctccttttt
     4921 agagttcacg attaatctac cctctatcct ccttctaccc attaatatga taaaatcatc
     4981 atgtatggta tttcggttta aatttaccat gataaatgat aaaatcaata atgagaagat
     5041 ttaagggaat cataaaagag ctattctttg tatgaggagg atgcctatgc ttgaatatga
     5101 aaagaagctt aaggattaca ttgccaataa taatattgat gcagaccacc tgcattttga
     5161 caagatctgt cactctgtcc aggaggctgc cgatgcagca ggtgcaacac cagatgatct
     5221 tgtcaagaat atctgtatgg ttgatgataa gggtaatctt attgtagcca ttgtcaaggg
     5281 cgaagacagg gtgagtacat caagggttgg caaggttctg aaaattgaac ggcccagaac
     5341 agcaactgag gaggaaatac ttgaaaagac agggtttcct tgtggtggag ttccatcatt
     5401 cggctaccat gccaccttta ttgtggatcc aagggttatg gaaaaggagt atgtctattc
     5461 tggaggcggg tctgagtatt ccctggtacg tatgagtagt caggagctga ccagggtcaa
     5521 cagtggaatc ataacgagag tgaggaaatg attatatgga gggtacaacc cgtaatagca
     5581 ttatgttagg aacgaaagta agtattgttc aaaaacagga ccagaggtcg ggagcactca
     5641 cccagggagt tgtagctaag atcttgacaa aatcccaaac ccatcctcat ggtattaagg
     5701 ttatgctcca ggatggaact gtaggcagag tgaaggaaat attggaagac taggcaggtg
     5761 atgatgtgaa tatacagata tatggggcta acaagtgctt tgatactaaa aaggcagaaa
     5821 ggtactttaa ggaaaggaaa ataaaattcc agtacaagga cctgtacagg tacggactga
     5881 gcaagggaga gtttgataac ataaaagctg ccgttggaat ggagagccta ataaatacaa
     5941 agtcaaagga ctacgagaag ctgaatctgc acaaaataag agggaatgca attcgcgagg
     6001 agattatcct tgagaacccc aagcttttca atactccaat tgtaagaaac ggcaagcagg
     6061 caacagtggg ttacaagcct gaaatatggg aggactggga gtagttgggt atgattatta
     6121 aaaaggagag gtgatcttac atgaagttaa cctatcttgg acattcttca ttcttggtgg
     6181 aggagggaaa attcaaagga gtagtggacc ccttcctgtc tggaaaccag gcttccacca
     6241 caaggcctga tgatataaag gatttgaccc atgtctttat aacccacggg catggggacc
     6301 acattggtga tgctgtccag cttgcaaagg caaatgatgc tctggttatc accaatgccg
     6361 aaatagctga ttatctttca tcaaagggat taagggtcca cgccatgcat attgggggga
     6421 gattcaggtt tgattttgga acggtcaaga tgactccagc cctccacggg tctggaataa
     6481 agacagacaa gggaatgctt tatggaggaa atcccggagg atttgtcatt gaggtaaatg
     6541 gcaagaaggt ataccatgca ggtgatactg gacttaccat ggac
//
LOCUS       DASRQF010000066         6502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_578865, whole genome shotgun sequence.
ACCESSION   DASRQF010000066 DASRQF010000000
VERSION     DASRQF010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6502
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578865"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1771
                     /gene="lon"
                     /locus_tag="VFD79_01730"
     CDS             <1..1771
                     /gene="lon"
                     /locus_tag="VFD79_01730"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HZH92797.1"
                     /translation="RLEILHEILQREIELLRIEEKINQRVKKQINKVQKEYYLKEQLK
                     AIQKELGEDDTFSSEMEEYASKIKKLKMPKEVREKALKEVDRLAKMSPHSAEVGVIRT
                     YLDWIVDLPWDKETKEKIDIKKAREILDNDHYGLMDVKERILEFIAIRKLTNSIKGPI
                     LCLVGPPGVGKTSIARSIASSLNRNFVRMSLGGVRDEAEIRGHRRTYVGSMPGRIISS
                     IKKAGTRNPVFLFDEIDKLASDFRGDPASALLEVLDPEQNSTFTDHFLEAPFDLSKVM
                     FITTANTTSTIPPALLDRMEVIRISGYTEEEKLQIAKRYLLPKQMKEHGLKDDQIKIS
                     DAAIRGVINYYTREAGVRNLERNIANLCRKAAKKIVEDGKKSVSINAGNITTYLGTKK
                     FRYDKLEEKNQVGVANGLAWTAVGGETLSVEVTTMKGTGKTQLTGKLGDVMKESAMAG
                     ISYVRANAERLKISPDFYKDVDIHIHVPEGAIPKDGPSAGITIATAVISALTERAILR
                     EVAMTGEITLRGRVLPVGGIKEKLLAAHRMGIKKVLLPYDNNKDLEEIPDKIKKKMEF
                     VLVKDLDQVLEHALEKGETNEDN"
     gene            1758..2360
                     /gene="yihA"
                     /locus_tag="VFD79_01735"
     CDS             1758..2360
                     /gene="yihA"
                     /locus_tag="VFD79_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HZH92798.1"
                     /translation="MKIISSDLQAMAVAPKGYPDDGMPELAFAGRSDVGKSSFINAML
                     NRTNLARTSGKPGKTRTINFYLINEKFRFVDLPGYGYAQVSKSERKKWGDIIEKYLRK
                     RDNLKAIVLLVDIRHKPSGQDIDMYHWIKTFGYDAIIVATKADKIARGQWERHMKIIR
                     DELEIEGNDFVVPFSSSKKLNVDKLWEIFEPMLVQNPIEE"
     gene            complement(2410..2568)
                     /locus_tag="VFD79_01740"
     CDS             complement(2410..2568)
                     /locus_tag="VFD79_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HZH92799.1"
                     /translation="MDKWVCGPCGYVYDPAEGDPDGGIAPGTAFEDLPEDWVCPVCGA
                     TKDMFEKE"
     gene            2730..3119
                     /locus_tag="VFD79_01745"
     CDS             2730..3119
                     /locus_tag="VFD79_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004825091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZH92800.1"
                     /translation="MNYIYKGTVCSDLETIKNFIDKTVNNLDSLISDRDLIFDIKVIL
                     NELIVNGALHGNECVSSKCVSLTLKMSQDKLTIEVQDEGNGIQYDPNEYNPSDLKSWG
                     RGLVLVNGLSDEFYVDRNKVVSVKNIV"
     gene            3264..5078
                     /gene="typA"
                     /locus_tag="VFD79_01750"
     CDS             3264..5078
                     /gene="typA"
                     /locus_tag="VFD79_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019108354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HZH92801.1"
                     /translation="MKRNDIRNVAIIAHVDHGKTTLVDSLLKQSGIYRENQVVQERVM
                     DSNDIEKERGITILAKNTAVTYNGIKINIVDTPGHADFGGEVERVLKMVNGVVLLVDA
                     FEGAMPQTKFVLRKALELELPVVVAINKIDRPEARPEEVIDEILDLFIELGAKDSQLD
                     CPFVFTSAKEGTATLDLEDSTESMKDLFETIVEYIPAPEGESDSPTQILISTIDYNEY
                     IGRIGIGKVERGVIHRNQEVIIVNKAKPDRKVKAKINKLYEFEGLNRVEVETSTIGSI
                     VALSGPADLEIGDTLCDVDTPEAIDFVKISEPTLAMTFSVNNSPFAGREGDFVTSRQV
                     RNRLYKELQTDVSLRVEDTDQTDAFKVSGRGELHLSVLIENMRREGFEFQVSKPEVLY
                     KTIDGRKHEPMEKVTIDVDSEFVGAVIDKLGQRKGELVSMSEPVGGYARLIFSIPARG
                     LIGYRQEFLTDTKGNGILNTIFDGYAPYKGDIPRRKSGSIIAFETGEATGYGLYSAQE
                     RGILFIEAGKAVYEGMVIGSNPKGLDIEVNVTKRKQQSNVRASGSDDSLRLSPPKIMS
                     LEEALEFIEEDELIEVTPLNFRVRKRILETNKRYKSKR"
     gene            5107..>6502
                     /gene="cls"
                     /locus_tag="VFD79_01755"
     CDS             5107..>6502
                     /gene="cls"
                     /locus_tag="VFD79_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HZH92802.1"
                     /translation="MESLFSSYMFIFSNLIWINILLALVLVFFERRNPTSTWLWLMVL
                     LFLPGIGFIFYLFLGQDMSKKKIFKTKAEEDDCFDILIADQEKNFSTRDFLKRNPEYK
                     SYNDIVKLHLMGSNSVFTSDNNVRLYFSGEDKFEDLLKSIAGAEDYVHMEYYIFNDDN
                     LGNEVMEALAKKASEGIEVKLLIDGMGSRKLGRSLTNVLREAGGEVVYFFPPFIPVFN
                     VRINYRNHRKICIIDGKEAYLGGFNVGDEYLGLSRKFGYWRDTHIRIEGSAISSIQWQ
                     FFLDWRFASGEEIRGCQSYIGDRGENDQIGIQVVSSGPDSKWPSIKDGYLKMINKAEN
                     RLYIETPYFIPDDSIFEALKIAGLSGLDVRVMIPNKPDHPFVYWAGLSYIGELLEAGV
                     RFYTYERGFLHSKVIIMDDLVSTVGTANLDIRSFKLNFEINAFLYDKDINQQLAERFI
                     RDLDNCREITMETYK"
BASE COUNT         2144 a         1147 c         1595 g         1616 t
ORIGIN      
        1 acggctggaa atcctccatg agatactaca aagagagatt gagctgctga ggattgagga
       61 gaaaatcaac caaagggtca agaagcagat aaacaaggtc cagaaggagt attatctgaa
      121 ggagcagctt aaggccattc agaaggagct tggagaagat gacactttct caagcgaaat
      181 ggaggaatat gcaagcaaga tcaagaagct aaagatgcca aaggaagtca gggaaaaggc
      241 attgaaggaa gttgacaggc tggccaagat gtcaccacat tcagctgagg ttggtgtaat
      301 cagaacatac ttggattgga tagttgacct tccctgggat aaggaaacaa aggaaaaaat
      361 cgacattaaa aaggcaagag agatccttga caatgaccat tacggtctta tggatgtaaa
      421 ggagagaatc cttgagttca tagccataag aaagcttacc aattctataa aagggccgat
      481 tctatgcctg gtaggacctc ctggagtagg taagacctcc atagccagat ccatagcatc
      541 ctcactaaac agaaatttcg ttaggatgtc acttggtgga gttagggatg aagctgagat
      601 taggggccac agaagaacct atgtaggcag catgccagga aggataataa gttcaatcaa
      661 aaaagctgga accagaaatc cagtatttct atttgatgaa atagacaagc ttgcaagtga
      721 cttcagagga gatccagcaa gtgcactttt ggaagttttg gaccctgaac agaactcaac
      781 attcactgac catttccttg aggctccatt tgatttgtcc aaggttatgt ttataacaac
      841 agcaaatacc acatcaacaa taccacctgc cctattggac aggatggagg taattagaat
      901 cagtggatat actgaggagg agaagctgca gatagccaag aggtacctgt tgccaaagca
      961 gatgaaggag catgggctaa aggacgacca gatcaagatt tcggatgccg caatcagagg
     1021 tgtgatcaac tactatacca gagaagccgg agtcagaaac ctggaaagaa acattgccaa
     1081 cctatgtagg aaagctgcca agaaaattgt tgaggatggg aagaaatcag tatctatcaa
     1141 tgctggcaac atcacaacat accttggaac taaaaagttc agatatgaca agctggagga
     1201 gaagaaccag gtcggtgtgg caaatggtct tgcatggact gctgttggtg gagagacact
     1261 ttctgttgag gtaaccacca tgaagggtac aggaaaaact cagcttacgg ggaaactggg
     1321 agacgtaatg aaggaatcgg ccatggctgg aatcagttat gtaagggcaa atgctgaaag
     1381 gcttaaaata tcaccggact tctacaagga tgttgatatt cacatacatg tacctgaggg
     1441 agcaattcct aaggacggtc cgtctgctgg aataacaatt gcaactgctg taatatccgc
     1501 attaactgag agggccattc taagagaggt agccatgact ggagagatta cccttagggg
     1561 aagagtcttg cctgttggtg gaatcaagga gaagctgctc gctgcccaca ggatgggcat
     1621 aaagaaggta ctcctgccat atgacaacaa taaggatctg gaggaaatac ctgataagat
     1681 taagaaaaag atggaatttg tcctagtcaa ggatttggac caggttttgg aacatgcgtt
     1741 ggaaaagggt gaaacaaatg aagataatta gttcagacct ccaggcaatg gcggtagctc
     1801 caaaaggata cccggatgac gggatgcctg agcttgcctt tgcgggcagg tccgatgttg
     1861 gaaaatcatc atttataaat gccatgctaa acaggactaa cctggcaagg accagcggaa
     1921 aacctggaaa gaccagaacc ataaacttct atctgatcaa tgaaaagttc agatttgttg
     1981 atttgccagg atatggctat gcccaggtct ccaaaagtga gcgtaaaaaa tggggagaca
     2041 taattgagaa gtatctaagg aaaagggata acctaaaagc catagttctc ctggttgata
     2101 tcaggcacaa accttctggc caggatatcg acatgtacca ttggattaaa acattcggtt
     2161 atgatgcaat catagttgcc acaaaggctg acaagattgc cagaggtcaa tgggagagac
     2221 acatgaagat aattagggac gagttggaaa ttgaaggaaa tgacttcgta gtgccttttt
     2281 catcatccaa gaagctaaat gtagacaagc tatgggagat atttgagccc atgctggtcc
     2341 aaaatccaat agaggaataa aaagacaaat aaagagccag tggtttccac tggctctttg
     2401 taagtacttt tactcttttt caaacatatc tttggttgct ccacatactg ggcaaaccca
     2461 atcctctgga agatcttcaa aagctgttcc tggagctata cctccatctg gatctccctc
     2521 tgctggatca taaacataac cgcatggtcc acatacccat ttgtccattg tgcattctcc
     2581 tttcgtttat gaatttactt agatacctta ttataccata tttttactaa aaggaaatag
     2641 gcaatttatt ttggggaaac tgttatatta gattataagc ccaaaatatg ggtaaaatac
     2701 gtatgactag aatatttgga tgtgataaga tgaattatat ttataagggt acagtctgta
     2761 gtgatcttga aaccattaag aacttcatcg acaagactgt aaacaatctc gacagcttaa
     2821 tatccgatag agaccttatt tttgatataa aggttattct aaatgaactt attgtcaacg
     2881 gagctctcca tggcaacgaa tgcgtgtcct caaaatgtgt cagtctgacc ctgaagatga
     2941 gtcaggacaa gcttacaata gaggttcagg atgaggggaa tggcattcag tacgatccca
     3001 atgagtataa tccttcagac cttaagtctt ggggaagggg tctggtcctt gtcaatggac
     3061 tttcagatga attttacgta gacagaaata aggttgtatc agttaaaaat atcgtttagg
     3121 cactgataaa ataccataag ttatcaaata aggtattact ccaaatagag taatactttt
     3181 tttgtgtttt atcgtaaagt gtgatagtat tataaagtta agcgtatgac aatttgataa
     3241 tagaagggat ctgtaataaa tctatgaaaa gaaatgatat tagaaatgta gcaataattg
     3301 cccatgtcga tcacgggaaa accacattgg tggatagcct acttaagcaa tcgggaatat
     3361 atagggaaaa ccaggttgtt caggagaggg tgatggattc caatgacatt gaaaaggaaa
     3421 gaggaatcac catactggca aaaaatactg ccgtcactta caatggcatt aaaattaaca
     3481 ttgtggatac acctggacat gcagattttg gtggcgaggt ggagcgagtc ctgaaaatgg
     3541 tgaatggggt ggtcctgctt gtggatgcat ttgaaggagc catgccgcag acaaaattcg
     3601 ttttgagaaa agccctggaa ctggaactac cggtagtggt tgcaatcaac aagatagata
     3661 gacctgaggc aaggccggaa gaggtaatag atgaaatcct tgaccttttc atagaattgg
     3721 gtgccaagga tagccagctg gactgtcctt ttgtcttcac atctgccaag gaaggcactg
     3781 caaccttgga cctggaggac agtactgaat caatgaagga cctgtttgag acgattgtag
     3841 aatacattcc agccccagaa ggagaatcgg acagtcctac acagattttg atatctacga
     3901 ttgactacaa tgaatacatt ggaagaatcg gtattggcaa ggtggaaaga ggagtaatac
     3961 acagaaatca ggaagtaata attgtaaaca aggcaaaacc cgatagaaaa gtgaaggcaa
     4021 agataaacaa gctctatgaa tttgagggct tgaacagggt ggaggtcgag acatccacca
     4081 taggaagcat agttgcactt tcaggtcctg ccgatttgga aatcggggat accctttgcg
     4141 atgtggatac tcctgaagcc attgactttg taaagatatc tgaacctaca cttgccatga
     4201 ccttctctgt aaacaacagt ccatttgcag gaagagaggg agattttgta acatcaagac
     4261 aggtcaggaa cagactatat aaggagcttc agacagatgt gagcctaagg gttgaggaca
     4321 cagaccagac agatgccttc aaggtttctg gaagaggaga actacacctg tctgttctaa
     4381 ttgaaaacat gagaagagaa ggttttgaat tccaggtatc aaagccggag gtcctttaca
     4441 agacaattga tggtagaaag catgaaccta tggaaaaggt gaccatagat gtcgacagtg
     4501 aatttgtcgg ggcggtgatt gacaagcttg ggcaacgaaa gggagagctt gtgagcatgt
     4561 cagaacctgt aggagggtat gcaagactga tattttcaat accagccagg ggtctgattg
     4621 gttacagaca ggaattcctt acggatacaa agggaaatgg catactgaat acgatttttg
     4681 atggatacgc accttataag ggagatattc ccagaagaaa atcaggctca ataattgcct
     4741 ttgaaactgg tgaggcaact ggttacggct tgtattcagc ccaggaaaga ggtatattat
     4801 tcatagaggc tggtaaagcg gtctatgaag gcatggttat cggctcgaat ccaaagggat
     4861 tggatataga ggttaatgtg acaaagagaa agcagcagtc taatgtaaga gcctctggtt
     4921 ctgatgattc cttgagattg tcgcctccaa agatcatgtc cctggaggaa gcccttgagt
     4981 tcatagagga agatgaattg atagaggtaa cacctttgaa tttcagggta cgaaagagaa
     5041 tattggaaac aaacaaaaga tacaaatcaa aaagataata tagagaacta gaaatgggag
     5101 gctgaaatgg aaagcctgtt ttcatcctac atgttcatat ttagcaacct tatatggatc
     5161 aatatactac ttgccttggt gttggtgttc ttcgagagaa gaaacccaac atcaacatgg
     5221 ctgtggctta tggtcctgct ttttttgcct ggtatcggat tcatattcta cctgttcctg
     5281 ggccaggata tgtccaagaa gaagatattc aagaccaagg cagaggagga tgattgcttc
     5341 gacatactca ttgccgatca ggaaaaaaac ttcagcacaa gggattttct taaaagaaac
     5401 ccagaataca aaagctacaa cgacatagta aagctccatt taatgggcag taattcagta
     5461 ttcacatcag acaacaacgt caggttgtat tttagcggcg aggataagtt cgaggacctt
     5521 ttaaagagca ttgcaggtgc ggaagactat gtccacatgg aatactatat attcaatgat
     5581 gacaatcttg gaaatgaagt gatggaggct cttgcaaaaa aggcttcaga gggaatcgag
     5641 gtcaagcttc ttattgacgg aatgggaagc agaaaactag gaagaagcct gaccaatgtt
     5701 ctgagggagg ctggcggtga agttgtctac ttctttcctc ccttcattcc ggtcttcaat
     5761 gtgaggataa actaccgaaa ccataggaaa atatgtatca tagacggtaa ggaggcatac
     5821 ctggggggct ttaatgttgg tgacgaatac ctggggctat cccggaagtt cggatattgg
     5881 cgagataccc acataaggat tgaagggtcg gctatcagtt ccattcaatg gcaattcttc
     5941 ctggattgga gatttgcttc aggagaggag ataagaggct gtcaaagcta cattggtgac
     6001 aggggtgaga atgatcagat tggaatacag gttgtgagca gtgggcccga ctccaaatgg
     6061 ccaagcatca aggacggcta ccttaagatg atcaataaag ccgaaaacag actttatatt
     6121 gaaacaccat acttcatacc agatgatagt atctttgagg cattgaaaat tgctggattg
     6181 tctggattgg atgtaagggt catgattcca aacaagccag atcatccctt tgtatattgg
     6241 gcgggattaa gctacatagg ggagcttttg gaagctggag taaggttcta tacctacgag
     6301 aggggattcc tacactcaaa ggtcatcatt atggacgatt tagtctctac ggtaggaacg
     6361 gccaatcttg acattcgtag ttttaagctc aattttgaga taaatgcatt tctttatgac
     6421 aaggatataa accaacagct tgcagaaaga tttattaggg acttggataa ctgccgggag
     6481 ataacaatgg aaacctacaa at
//
LOCUS       DASRQF010000067         2630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1695671, whole genome shotgun sequence.
ACCESSION   DASRQF010000067 DASRQF010000000
VERSION     DASRQF010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1695671"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VFD79_01760"
     CDS             complement(<1..186)
                     /locus_tag="VFD79_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZH92803.1"
                     /translation="MLSKVALTAEHGELVALLGPSGCGKTTLLRLVAGLMQVQQGSIR
                     FGDKDVTKMDPRRRNAVM"
     gene            complement(253..1095)
                     /locus_tag="VFD79_01765"
     CDS             complement(253..1095)
                     /locus_tag="VFD79_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92804.1"
                     /translation="MRIEFHTDTVKTSNEDIYGLTKRGAFVIDGASALTSRSFVPEGN
                     DVSWMVQWWRDYLEEHLDDITFTIQEILKDGIRNFNKDYGRFVDLANLQPHEQLSAGI
                     AILRKNGNILESYVLGDVEISVEGRNGDCSIVTDQALKGLDAEVIELMRRNHEREKQV
                     VFKGFTEKELEILVRNRKKMNVAGGYYILGHSVEAVDMGIFKTFQIDEIERCLLSTDG
                     IVPLNYKYNRSNLLDRIRSKGVREIIRELRGMEESDREKRSMGRLKTHDDATVIYLDF
                     GLQS"
     gene            complement(1108..2580)
                     /locus_tag="VFD79_01770"
     CDS             complement(1108..2580)
                     /locus_tag="VFD79_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HZH92805.1"
                     /translation="MAIKNTLIMGVLTVVICGFIGTFLAFFVHYFDFPFKRLLDRVLL
                     LPLVVPGLIIVFAFVQLYGESGMVTKTIENLLGLDNQPYTFMGLPGILMVHAYTQYIY
                     FYMNVSVAVKELDRSVVDAAINLGASPLRVFRTVIVPYIAPALISSGILTFMTGVGSF
                     SAPNIIGGGFKVLTTQILLAKANLYMDLAAAQVVVLSVFAMSYMGIGRYYERKVSFQT
                     GTREHLIQPVKIRNNAMRWIMSLVAVGIVFMIFLPIITIIVLSFVKPGTWMIEIFPKE
                     FGFDNYIRIFTRSRSLAPFINSLKMALFAAFTAVALAVPAAYTVTKTKTRLKPIIEFL
                     LMLPFALPASAIAINMINGFSSVLLGKWIMLPLAYFVSMLPMAVRSVTISYQKLKDQY
                     SEASTNLGAGGITTLSKIVLPLISPGIWSGFLLVFIRSLGEYTISAFLYNASNRPISI
                     AMVNSMFEFEIGLAMAYGALVLLLTAMGSSVLKRLQGKIG"
BASE COUNT          764 a          643 c          431 g          792 t
ORIGIN      
        1 catgacagca tttctcctac ggggatccat tttggtaaca tctttgtccc caaaacgtat
       61 gctcccctgc tgcacctgca ttaaacccgc tacaagtctc aacaaggtgg ttttcccaca
      121 ccctgatggc cccaagagtg ccaccagttc accatgctct gcagtgagtg ctactttgct
      181 taacactcta ttttctccgt aattatgggt aatatcactt atcctgatat ctatgagtat
      241 cacctgcctt aatcatgact gaagtccaaa atccagataa atcactgttg catcatcatg
      301 ggtcttgagt cttcccatgc tcctcttttc cctgtctgat tcctccatgc ccctaagttc
      361 ccggattatt tcccttacac ctttgcttct tattctatcc agcaagttgc tcctgttgta
      421 cttatagttc aagggcacaa taccatctgt agatagaaga cacctttcaa tttcatctat
      481 ctggaaagtc ttaaatattc ccatatcaac agcctctaca ctatgaccta atatataata
      541 tcctccagct acattcatct tcttcctgtt tctgacaagt atctcaagtt ccttttcagt
      601 gaaacctttg aataccacct gcttttcacg ttcgtggttt cttctcatca gttcaatcac
      661 ttctgcatca agacccttca atgcctggtc agttactatc gagcaatctc cgtttcttcc
      721 ttcaactgaa atttcaacat ctccgagaac gtaagactca agaatatttc catttttcct
      781 aaggattgca atacccgcag atagttgttc atgtggttga agattagcaa ggtctacaaa
      841 ccttccataa tccttgttga agtttcttat tccatccttg agtatttcct gtattgtaaa
      901 ggttatatca tccaagtgtt cttcaagata gtctctccac cactgcacca tccagctcac
      961 atcatttcct tcaggaacaa agctcctgct tgtcaatgct gatgcaccat caattacaaa
     1021 tgctcctctc ttagttagac catatatgtc ctcgttgctt gtctttacag tgtctgtatg
     1081 aaattcaatc ctcatgttcc acctcatcta tccaatcttt ccctgaagtc ttttcaatac
     1141 agaagacccc atcgcggtca ataggagcac cagagctcca taggccattg ccaatccaat
     1201 ttcaaactca aacatgctgt ttaccattgc aatggaaatt ggcctgtttg aagcattata
     1261 taaaaatgca gatattgtat actcacctaa gcttctgata aaaaccaaaa gaaaaccaga
     1321 ccaaattcct ggagaaataa gtggcagtac gatctttgaa agagtagtta ttccacctgc
     1381 acccagatta gttgaagcct cggaatactg gtcctttaac ttctggtatg atatggtcac
     1441 tgaacgtaca gccatgggaa gcatgctcac aaaatatgcc aatggaagca ttatccactt
     1501 tcctaaaagt acagagctaa atccattgat catatttatt gctattgcac tggcaggcaa
     1561 tgcaaatggc agcatcagaa gaaactcaat tatcggcttc aatcttgttt tagtctttgt
     1621 aacagtatat gctgctggaa ctgccagggc aacagcagta aatgctgcga acaaagccat
     1681 cttaaggcta tttataaaag gagcaaggct cctgcttctt gtaaatattc tgatgtaatt
     1741 atcaaagcca aactctttgg ggaatatttc aatcatccag gttcctggct ttacaaagga
     1801 taaaacaatg attgttatta tgggaagaaa tatcatgaaa actatcccca ctgccaccaa
     1861 ggacattatc catctcatgg cattattcct tatttttaca ggttgtatca gatgttccct
     1921 ggttcctgtc tggaatgata ccttcctctc gtagtacctt cctattccca tgtagctcat
     1981 tgcaaatact gaaagcacaa caacctgagc tgctgcaagg tccatataca gatttgcctt
     2041 tgccaataaa atctgtgttg taagaacctt gaatcctcca ccaattatat tgggggctga
     2101 aaatgatcca accccagtca taaaggtcag aattccagaa cttatgagcg ctggagctat
     2161 atatggcaca ataactgtcc tgaacactct taggggtgat gcccccagat tgattgccgc
     2221 atcaacaact gacctatcca attcctttac ggcaactgaa acattcatat aaaaatatat
     2281 gtattgggtg tatgcgtgta ccattagaat tccaggcaga cccatgaagg tatagggttg
     2341 attatccagt cccaacaagt tctctatggt ctttgttacc attccacttt ctccgtaaag
     2401 ctgcacaaat gcaaacacaa ttatcaagcc tggcaccacc aagggcaaca acaatactct
     2461 atcaaggagc ctcttaaatg ggaaatcaaa gtaatgtaca aaaaatgcca gaaatgtccc
     2521 tatgaagcca cagattacaa ccgtgaggac acccatgatc aaggtgtttt ttatagccac
     2581 tagagaaccc cctgtactga atatatattc atagttttca agggaaaacc
//
LOCUS       DASRQF010000068         5124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1823839, whole genome shotgun sequence.
ACCESSION   DASRQF010000068 DASRQF010000000
VERSION     DASRQF010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1823839"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..797)
                     /locus_tag="VFD79_01775"
     CDS             complement(87..797)
                     /locus_tag="VFD79_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005956756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZH92806.1"
                     /translation="MEKPRILVVDDDREIAAAIEKLLVLEDFFVVKAYDGMEAMDILI
                     GEDVHLIIMDVMMPRLDGLSATMKIREERNIPIIILSAKSQDSDKILGLSMGADDYVT
                     KPFNPQELVARVRSQLRRYMDLGDVGSSRKESGLRVGSLYLDEESRELLVDGENVRLT
                     PTEYGITQLLMKNPGVVFSAEQIYEKVWKEEAYSVENTVMVHIRRIREKIEINPKEPK
                     YLKVVWGIGYKIEKLQQK"
     gene            complement(880..1800)
                     /locus_tag="VFD79_01780"
     CDS             complement(880..1800)
                     /locus_tag="VFD79_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZH92807.1"
                     /translation="MTENRFLPYMTALGYSMIFGMSFMFTKIGLGYMTTLQLIAYRFL
                     VAATAMQVLRALKLIRLDFKGKDLKLVIMTAIFQPVLYFFFEVEGVNLTSSSQAGLMV
                     SLVPVTTAIGARIFLKERLKPIQIMFIALSVGGVMVINLLNDSSGLGGNTLGLVFLTM
                     SVISGTLYSLSSKRASSQFSPVEITYTMMIIGAVVFNGTLLARSLFEGTLAIYFQPLI
                     NPEVYGPILYLGLLSSVVAFFFLNFTISRIPVSQSAIFGNLVTVFAILAGVFILNEPF
                     GIKDVIGAIMIVTGVWGTLYFGPKKKRARD"
     gene            complement(1895..2596)
                     /locus_tag="VFD79_01785"
     CDS             complement(1895..2596)
                     /locus_tag="VFD79_01785"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZH92808.1"
                     /translation="MALRNINLKVRDQEFICLIGPSGSGKSTLLRLMEGLIFPTSGCI
                     RVQGAEVKGPVDNAGFVFQEYSLMPWRTIIDNISFGLEIKGESKKDRYELSKRYLSRF
                     GLEGFENNYPYELSGGMKQRAAIARAIVVSPKILFMDEPFGALDAYTRIQMQNDLIDF
                     WMEEKRTVIFVTHSVEEAVFLGSRVLLMSPRPGEISKEYIIDNPYPRSRFDEKFRGHF
                     QMIMEDMEKVSKHSN"
     gene            complement(2626..3384)
                     /locus_tag="VFD79_01790"
     CDS             complement(2626..3384)
                     /locus_tag="VFD79_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002839581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HZH92809.1"
                     /translation="MHYIGIDIGSTAAKVTVFDNEKIMDNFTLPTGWSSVETSRIIKN
                     RLEDSGIEVEKSKVVATGYGRISVPYAEKTITEISCHGEGVKYLLGEDVTIVDIGGQD
                     TKIITLENGRVTDFIMNDKCAAGTGKFLELMANTLGVDIKELTDMALEGRDISITSMC
                     TVFAESEVISLIGQGTDRSSISRGIINSITGRVNSLLHKQVRKDKVFLTGGLCEMESF
                     ILLLSQKVGVKVETHKLARYAGSIGAAILAGNLE"
     gene            complement(3407..4555)
                     /locus_tag="VFD79_01795"
     CDS             complement(3407..4555)
                     /locus_tag="VFD79_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HZH92810.1"
                     /translation="MRPKVMEEVESLRGINSVKIKEAHEEGKKIVGMYCVFSPSEIAL
                     AADAISVTLCGTSQDPIEEAEKTLPRNLCPLIKSSYGFAVTDKCPYFYFSDVLLAETT
                     CDGKKKMYELMGEIKPMHIMNLPQTANDEDSLEFWEKEMVKFKNFLENQFSLEITDEK
                     LSDAIKLMNRERKALRELHSLNSHKPAPLSGMDMMLAQWLKGFNVDKEAGISIIEKLI
                     QETKDNVSKGLFAFDESAPRILLTGTPIGVGSEKVLRALEEAGASVVALENCTGYKGL
                     DVLVDESKDPITALAEKYLSTPCSCMTDNNGRLDLIGRLCEEYKVDGVVDLTWQACHT
                     YNIESYTVKKYVQEQLKLPFMQVETDYSDSDTGQVKVRAEAFVETLKI"
     gene            complement(4717..>5124)
                     /locus_tag="VFD79_01800"
     CDS             complement(4717..>5124)
                     /locus_tag="VFD79_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZH92811.1"
                     /translation="PDFTLLDMEGNQVKLSDFEGRMVFLNFWATWCTYCDIEMPDLQK
                     VNDEHEDLVVLAVNVREDKDLVQEYLDEGGYDFPVVLDEDGHMAQIYLVSGMPTTYFI
                     SDDGKLLGYQPGMLTLEQMEDIIVQMRDLQDQL"
BASE COUNT         1455 a         1282 c          812 g         1575 t
ORIGIN      
        1 tccctgaaaa ataccaataa acaaatattc cagacatgcc agccaccatg gcaactataa
       61 gtaaaaacga tgccagcttt gtccagttac ttctgctgaa gcttttcaat cttgtatcca
      121 attccccaca ccaccttaag atatttaggt tcttttggat tgatttcgat tttctcccgt
      181 atccttctta tatgtaccat tacagtgttc tccaccgaat aggcctcttc cttccagacc
      241 ttctcataaa tctgctcagc tgaaaacacc actcccgggt ttttcataag aagctgcgta
      301 atcccatatt cagtaggtgt aagacgtaca ttttcaccat ccaccaaaag ctcccttgac
      361 tcttcgtcaa gatacaggct cccaacccta agtccggact cctttcttga gcttcccaca
      421 tcacccaggt ccatgtacct tctcaattga ctccttaccc tggctaccag ttcctggggg
      481 ttgaagggct tggtgacata atcatccgcc cccatggata gtccaaggat cttgtcgctg
      541 tcctgggact tggctgacag gattattatt gggatgtttc tctcttctct gattttcatt
      601 gtggctgaca gtccgtccag cctgggcatc atgacatcca tgataataag gtgaacatct
      661 tcacctatga gaatgtccat ggcttccatg ccatcatagg ccttcaccac aaaaaaatcc
      721 tccaagacca ggagtttctc aatggccgct gctatctccc tgtcatcgtc cacaaccaga
      781 atacggggtt tttccattaa atcttcacca tcctttacct taatcctaag tataagggat
      841 aattcttaca aaacagtcca cacatttctt acaaaattct taatctcttg cccttttctt
      901 ctttggaccg aagtaaaggg taccccaaac tcctgtaaca atcattattg cacctattac
      961 atcctttatc ccaaagggtt cattgagtat gaatactcct gcaagtattg caaataccgt
     1021 tacaaggttt ccaaaaattg cagactggga tacaggtatc ctggatatcg tgaagttaag
     1081 aaagaaaaag gccacaactg aagacaagag tcccaggtat aggattggtc cataaacctc
     1141 cggatttatc agtggttgaa aatatattgc cagggttccc tcaaagaggc ttctcgcaag
     1201 aagtgtacca ttgaagacta ccgcaccaat tatcatcatg gtataggtta tttcaacagg
     1261 tgagaactgg ctggaggctc tttttgagct taggctatac agggttcctg aaattaccga
     1321 catggtcaga aaaacaagcc caagtgtgtt cccacccaag ccgcttgaat cgtttagaag
     1381 gtttataacc ataacaccac caacagaaag tgcaataaac attatctgta tgggcttgag
     1441 cctttccttt aagaatatcc tggctcctat ggctgtggtg acaggaacaa gggataccat
     1501 cagtccagcc tgtgacgagg atgttaggtt tactccctcc acctcaaaga agaaataaag
     1561 caccggctgg aatattgcag tcatgattac aagcttcagg tcctttccct tgaaatcaag
     1621 cctgataagc ttcaaggccc tcaggacctg catggctgtt gcagccacca gaaatctgta
     1681 ggcaataagc tgcagggttg tcatgtagcc caagcctatc ttggtaaaca taaaggacat
     1741 tccaaatatc atagaatatc ccagtgcggt catatagggt aaaaatctat tttctgtcat
     1801 aatatcacac tcctcataca aatatagcat tttagggaga aactggcaat ggaaaagacc
     1861 gcttctcatt atctgtagaa gcagtctttt caacctaatt tgaatgtttg ctaacttttt
     1921 ccatgtcttc cattatcatc tgaaaatggc ccctaaattt ctcatcgaat ctacttctgg
     1981 gatagggatt gtcaattata tactccttag atatttctcc aggtctggga ctcataagaa
     2041 gcacccttga ccccaagaaa acagcctcct ccacactatg agtgacgaat ataactgttc
     2101 ttttctcttc catccaaaaa tcaatcaggt cattctgcat ttgaatccta gtatatgcat
     2161 ctaaagcacc gaagggttca tccataaaca atattttggg tgacacaaca atagctctgg
     2221 caatggctgc tctctgtttc attcctcccg acaattcata aggataattg ttctcgaatc
     2281 cctccaaacc aaacctactc aaatatcttt ttgaaagttc gtatctgtcc ttttttgact
     2341 cccctttaat ttccaatcca aaggatatgt tatcaataat tgtcctccat ggcatcaagg
     2401 aatactcctg aaaaacaaat cctgcgttat caaccggtcc ctttacttca gccccttgga
     2461 ccctgataca tccactcgtt ggaaatatca acccctccat aagcctaagt agtgttgatt
     2521 tacctgaacc actgggaccg atcaaacata tgaactcctg atctctcact ttcaggttga
     2581 tatttctaag ggccatcctt tctgaaaaaa caatatttga aattactact caagatttcc
     2641 tgcaagaatt gccgctccta tggagccagc atatctagcc aatttatgtg tttcaacttt
     2701 gactccaact ttctgagata gcagaagaat gaaggactcc atttcacaca acccaccagt
     2761 tagaaacact ttgtccttcc tgacttgttt atgcaaaagt gagttcaccc ttccagttat
     2821 agaattaatt atccctcttg aaatactact tctgtcagtg ccttgaccaa taagacttat
     2881 tacctctgat tcagcaaaaa ctgtacacat actagttatg cttatgtccc tcccttcgag
     2941 ggccatatct gtcaattctt ttatatccac accaagtgtg ttagccataa gttccaaaaa
     3001 cttacctgtg ccagcagcgc atttatcatt cataataaaa tctgtaaccc gaccattctc
     3061 cagtgtaata atcttagtat cctgtccacc tatgtcaact attgtaacat cctcgcccaa
     3121 aagatacttc acaccctcac catgacagct tatctcagta atggtctttt cagcatatgg
     3181 aactgatatt cttccataac cagttgccac aactttagat ttttcaactt caatgccact
     3241 atcttccaat cgatttttta ttatccttga agtctcaaca ctgctccaac ctgtgggcaa
     3301 ggtaaaatta tccataatct tctcattgtc aaatactgtt accttagctg ctgtagaacc
     3361 aatatctatt ccaatatagt gcatctatat catcctttat caaaaatcat atctttaatg
     3421 tttctacaaa agcttccgct cttaccttaa cctgaccagt atcagagtca gaataatctg
     3481 tttccacctg cataaatggt aattttaact gttcctgaac atacttcttc acagtatatg
     3541 attcaatatt gtatgtgtga catgcttgcc atgtaaggtc aaccactcca tcgaccttgt
     3601 actcttcaca tagtcttcca attaaatcta gtcttccatt gttatccgtc atacaggaac
     3661 atggtgttga aagatatttt tcagcaagag cagttatggg gtccttgctc tcatccacaa
     3721 gaacatccag tcccttgtaa cctgtacagt tctccaaagc cacaaccgaa gcacctgctt
     3781 cctccagagc cctaagtacc ttttctgatc caacacctat gggagttcct gtcaagagaa
     3841 ttcttggagc actttcatca aaggcaaata atcctttcga aacattatcc tttgtctcct
     3901 ggataagttt ttcaataatg cttataccag cttccttatc cacattgaat ccctttaacc
     3961 actgtgcaag catcatgtcc attcctgaca gtggggctgg cttatgtgaa ttcaaactat
     4021 gtaactctct aagagctttt ctttctctgt tcattaattt aattgcatca cttaactttt
     4081 catctgttat ttcaagactg aactgatttt ccaggaaatt tttaaacttc accatttcct
     4141 tttcccaaaa ctccagtgaa tcttcatcat tcgcagtctg tggaagattc attatgtgca
     4201 tgggctttat ctctcccatc agctcataca tttttttctt gccatcacaa gtagtctctg
     4261 caagaagtac gtccgaaaag taaaaatacg gacatttatc agtcactgca aatccatagc
     4321 ttgactttat caagggacaa agatttcttg gaagtgtttt ttcagcttcc tcaattggat
     4381 cctggctggt tccacaaagg gtaaccgaaa tagcatctgc agctagtgca atttcagatg
     4441 gcgaaaacac gcaatacata cctactatct tcttaccctc ctcatgagct tccttgattt
     4501 taactgagtt aatccctcta agtgattcaa cttcctccat aacttttggt ctcattttac
     4561 accctcctta ttcatagtca tcttgtccaa attatcacaa agccaatcat atttccaatt
     4621 acatattata atgggtaatc attatcaatt aattattctt atcacaaaaa aattctgcag
     4681 ccagttttac tcaactggct gcagaatttt gttcaattat agctggtctt gcaggtccct
     4741 catctgtact atgatatcct ccatctgctc aagagtaagc attcccggct gataccccaa
     4801 aagcttaccg tcatcactta taaaataggt tgttggcatt ccgcttacca ggtatatctg
     4861 tgccatatgg ccatcctcgt caagtacaac ggggaaatca tatccaccct catccaggta
     4921 ctcctggacc aggtccttgt cctcccttac attgactgca agtacaacca ggtcttcgtg
     4981 ttcatcattg accttctgaa ggtcaggcat ctctatgtca caataggtgc accaagttgc
     5041 ccaaaagtta agaaaaacca tccttccctc gaagtcgcta agctttacct ggtttccctc
     5101 catatcaaga agggtaaaat cagg
//
LOCUS       DASRQF010000069         2346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1204289, whole genome shotgun sequence.
ACCESSION   DASRQF010000069 DASRQF010000000
VERSION     DASRQF010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204289"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..>2346
                     /gene="leuS"
                     /locus_tag="VFD79_01805"
     CDS             67..>2346
                     /gene="leuS"
                     /locus_tag="VFD79_01805"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HZH92812.1"
                     /translation="MREYNPKDIEKKWQDIWDQEEAYKATNDSENPKFFALVEFPYPS
                     ANGLHVGHPRPYTALDIVARKRRYQGYNVLFPMGWDAFGLPTENFAIKNKIHPRIVTE
                     KNIERFKNQLKSIGFSFDWSREVNTTDPEYYKWTQWIFLKLFEKGLAYKQEFPINFCT
                     SCKVGLANEEVVGGVCERCGSEVVRRVKNQWMLKITEYAERLINDLDLVDYIERVKIQ
                     QTNWIGKSVGAELDFVISGGEDKLRVFTTRPDTLFGATYMVLAAEHPLIEKYADRIVN
                     IDQVRAYADEVLNKSDFERTELSKVKTGVELKGLTAINPATGKEIPLWVSDYVLMTYG
                     TGAIMAVPGHDQRDWEFARKFDLPIVEVIKGGDIEKEAYTDTDSGILVNSGFIDGMEV
                     KEAIRKMIDWLVEEGLGEKKVNYKLRDWVFSRQRYWGEPIPLVHCDDCGWVPIPEEEL
                     PLLLPEVDNYEPTETGESPLANIHDWVHTTCPKCGKDAHRETDTMPQWAGSSWYFLRY
                     TDPHNSEQFASDEALDYWMPVDWYNGGMEHTTLHLLYSRFWHKFLYDIGVVPGPEPYS
                     KRTSHGMILGDNNEKMSKSRGNVVNPDDIVNDYGADTLRTYEMFIGDFEKSVPWSENG
                     VKGCRRFLDRVWRLQEILVDGEGYTKDLESSIHKTIKKVSEDFETLKFNTGIAALMSL
                     LNEFNDHGSITRKDMETYLVLLNPVAPHITEELWEIVGIKGKLFQTTWPEFDVDKTID
                     KVIDLPVQVNGKVRGTITVA"
BASE COUNT          728 a          488 c          603 g          527 t
ORIGIN      
        1 cggaataacc tttcgtctct atggggatga aaggttttat tttttgaaat ttaaggaggt
       61 cattcaatga gagagtacaa tccaaaggat atcgagaaga agtggcagga tatctgggat
      121 caggaagagg catacaaggc caccaatgac agtgaaaatc caaaattctt tgccctggtg
      181 gagtttccat atccatctgc aaatggtctt cacgtaggac accccagacc atacactgca
      241 cttgatatcg tagccagaaa aaggagatac cagggataca atgtcctttt ccctatgggt
      301 tgggacgcat ttggactccc cactgagaac tttgcaatca agaacaaaat ccacccaagg
      361 atagtaacgg aaaagaatat cgaaaggttc aagaaccagc ttaagtcaat aggtttttcc
      421 tttgactggt caagagaggt caacaccaca gaccctgaat actataagtg gacccagtgg
      481 atattcctca agctttttga gaagggactt gcctacaagc aggaattccc aataaacttc
      541 tgtacatcct gcaaggtcgg tctggccaac gaggaggtag ttggcggggt ttgcgaaagg
      601 tgtggcagcg aggttgtaag gagggtcaag aaccaatgga tgctaaaaat aactgagtat
      661 gctgaaagac ttatcaatga cctggacctt gtggactaca ttgaaagggt caagattcag
      721 cagacaaact ggataggaaa atctgtcgga gctgagctgg actttgtgat ttcaggagga
      781 gaggacaagt taagggtttt tacaaccaga ccagacaccc tgttcggggc aacctacatg
      841 gtcctggcag ccgagcatcc actgattgaa aaatacgctg acagaattgt aaacattgac
      901 caggtaaggg cctatgccga tgaagttctt aacaagagtg attttgaaag gacagagctt
      961 tccaaggtca agaccggggt tgaattgaag ggcctgactg caatcaatcc agcaactggc
     1021 aaggagatac ccctttgggt atccgattac gtcctgatga catatggaac aggtgccatc
     1081 atggctgttc caggccatga ccaaagggac tgggagtttg caaggaagtt tgaccttcca
     1141 atagttgagg taataaaggg tggagacata gaaaaggaag catatacgga tacagattca
     1201 ggaattcttg tgaattccgg attcatagat ggaatggaag taaaggaagc catccggaag
     1261 atgatagact ggctggtgga agagggcctt ggagagaaaa aggtcaacta caaattgagg
     1321 gactgggttt tctcaagaca gagatattgg ggtgagccaa tacctctggt ccactgtgat
     1381 gattgtggct gggtgccaat tccagaggaa gagttaccac tcctccttcc tgaggtggac
     1441 aactacgaac caactgaaac cggggaatca ccacttgcaa acatacacga ctgggttcac
     1501 accacatgtc caaaatgcgg caaggatgcc cacagggaga ctgacacaat gccacaatgg
     1561 gctggatcat catggtattt cctaagatat accgaccccc acaacagtga gcagtttgca
     1621 tcagatgagg ccctggacta ctggatgcct gttgactggt acaatggtgg aatggaacac
     1681 acgacacttc acctgctcta ctccagattc tggcacaagt tcctgtatga cataggggtc
     1741 gtacctggac ctgagcccta ctccaagagg acatcccatg gaatgatcct tggagacaac
     1801 aacgagaaga tgtcaaaatc cagaggaaat gtggttaacc ctgacgacat agtaaacgac
     1861 tacggtgcag atacactaag aacctacgag atgttcatcg gagactttga aaagagcgtt
     1921 ccatggtctg aaaacggcgt taagggctgt agaagattcc ttgacagggt ttggagactt
     1981 caggagatac tggtggatgg agagggatat acaaaggacc ttgaatcctc catccacaag
     2041 accattaaga aggtaagcga ggactttgag acccttaagt tcaatacggg gatagcagcc
     2101 ctcatgtcac ttctcaatga gttcaacgat cacgggtcaa taaccaggaa ggacatggaa
     2161 acataccttg tactcctgaa ccctgttgca ccacacataa cagaggagct ttgggagatt
     2221 gtaggtatta agggaaaact cttccagaca acttggccag aatttgatgt ggataagacc
     2281 atagacaagg tcatagacct tcctgtccag gtcaacggaa aggttagggg taccataaca
     2341 gttgcc
//
LOCUS       DASRQF010000070         6246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_699195, whole genome shotgun sequence.
ACCESSION   DASRQF010000070 DASRQF010000000
VERSION     DASRQF010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699195"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="VFD79_01810"
     CDS             <1..755
                     /locus_tag="VFD79_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZH92813.1"
                     /translation="YLKKLGVRELEGVFISHFHEDHYKGLFPIIEELKIKALYVNNPI
                     PDMDLKNAVEMANIPVFKMEKGSRIDFQETSIECIFSSDGAYVNENNNSLVLLLESKG
                     KKVIFTGDIEAEVESQLPGYDIDLLKVAHHGSRTSSSEDCISRLSPEISVISAGRNNS
                     FGHPHDSVLETLGKFGSQVYRTDEAGLVKVIISDGEMMVIPYYGPPFQENLEEFIYNH
                     VHQLSWMLLYSILAYISVRTYAKMEEKDHEIQ"
     gene            742..1776
                     /gene="holA"
                     /locus_tag="VFD79_01815"
     CDS             742..1776
                     /gene="holA"
                     /locus_tag="VFD79_01815"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HZH92814.1"
                     /translation="MKYNEFQSLIENGNINPIYIFTGDETYLLDKAVEYLVGFFMTPE
                     GRTINLENLDGKTAGLDDLKASCETLPFMSERRVTILRGAEALLDRDSASDEVLNYIK
                     SLGDHQLLLVNDRDGKVKKNSKLYRYLNKSGSAVVFEKLRGKELTDWIQGKLSQRGKT
                     MTYADLNYFIQQSSYNSRNITTTLYDLDNEIKKLCDFSSGKNIDRNTLDQVLIRPLDK
                     NIFDFLGAVGKNDTDRAMEVFNEIYLMNEPVPRILFMISRQFRLLLGYVTYREKGYGS
                     GEIQEKLKVKPYEFGKIAAQGNGMDQNRLREILEDILQTDRNLKTISSDQRLEMEMLI
                     VKLTAGCKTY"
     gene            complement(1822..2085)
                     /gene="rpsT"
                     /locus_tag="VFD79_01820"
     CDS             complement(1822..2085)
                     /gene="rpsT"
                     /locus_tag="VFD79_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HZH92815.1"
                     /translation="MANIKSAEKRILVTAKKTALNKSKKTEIKTYIKKFESAVANGNF
                     DEARALLKVIDKKLKRAAHKNIVHDNAAARKISRLTKALNKAM"
     gene            2298..2849
                     /gene="lepB"
                     /locus_tag="VFD79_01825"
     CDS             2298..2849
                     /gene="lepB"
                     /locus_tag="VFD79_01825"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZH92816.1"
                     /translation="MSEMTEKRKEKSEAMEWVKSILAAIAIALIIKTFVFNTTYVLGN
                     SMYPTLHEQDRLFALKVPLYFKGPDRGDVIVLEAPDSPGKDYIKRVVGISGDKVEIQD
                     GMVYVNDVLLMEDYLQGEQYTHTYQENIWIVPEGDVFVLGDNRAEGASKDSRYFGTIS
                     LDKVKGITQFRYYPIDDRFGRIE"
     gene            2899..4710
                     /gene="lepA"
                     /locus_tag="VFD79_01830"
     CDS             2899..4710
                     /gene="lepA"
                     /locus_tag="VFD79_01830"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019034747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HZH92817.1"
                     /translation="MRIKQENIRNFSIIAHIDHGKSTLADRLIEMTGMLTSREMQEQV
                     LDKMDLERERGITIKLKAVRLVYKAKDGQEYVFNLIDTPGHVDFNYEVSRSLAACEGA
                     VLVVDATQGVEAQTLANVYLAIDQDLEILPVINKIDLPSARPEEIINEIEDIIGIESQ
                     DAPLISAKDGLNVEEVLEQIVEKVPAPKGDPNAPLKALIFDSYYDSYKGVTCYIRVFE
                     GQVKPGMMVKMMATGKSFEVTEVGVNTSKHIPLDSLSAGEVGYLTASIKNVKEARVGD
                     TITDTGNPTANPLPGYKKVIPMVYCGIYPAEGEDFNTVREALEKLQVNDAALVFEPET
                     SVALGFGFRCGFLGLLHMEIIQERLEREFDLNIITTAPSVIYRVTRTSGEVMEIQNPS
                     NLPAPAEIAHMEEPIVSANIMTPTDYVGAIMELCQNKRGTFKNMEYLEETRVVLHYDL
                     PLNEVIYDFFDALKSKTRGYASFDYELKGYQQSELVKMDILINSELVDAFSLIVHEEK
                     AYERGRTIAERLVDVIPRHQFAVPIQAAIGSKIIARETIRALRKDVLAKCYGGDISRK
                     RKLLEKQKEGKKRMRQIGSVDVPQEAFLTVLKYDEKK"
     gene            4772..5908
                     /gene="hemW"
                     /locus_tag="VFD79_01835"
     CDS             4772..5908
                     /gene="hemW"
                     /locus_tag="VFD79_01835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HZH92818.1"
                     /translation="MEKAGLYIHIPFCERKCHYCDFTSVAGNRDLIDRYVRYLINEIK
                     MVSAAYGKIQIETIFIGGGTPSVLSESSLETIMEAVGNGFSLDRNAEISMEINPGTLT
                     HQKALGYRKAGINRASVGIQSMNNSLLGTIGRIHNEDQVIATVKNLRRAGFDNINGDL
                     MFGLPHQSIDDFRVTLEKTALLDLEHISMYGLILEENTPLYGWHERGLVSVPTEDQER
                     EMYHLGIDFLKKKGYLQYEISNLAKKGHECRHNMGYWLLKPYIGVGISSHSSMEGRRY
                     WNTSGFGEYFQKLDEDTLPIEGEETITKDMMETEYLILGIRLKDGISVDDYEKRFKAS
                     FMGKYGEAMAKHMDSGLLVLTDGSIRLTEKGMDLSNQVEMDFIP"
     gene            6019..>6246
                     /locus_tag="VFD79_01840"
     CDS             6019..>6246
                     /locus_tag="VFD79_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HZH92819.1"
                     /translation="MLDERKMRVLYAIIDSYISTAEPIGSRTISKGYDLGVSSATIRN
                     EMSDLEELGYLNKTHTSSGRVPSDKAYRLYVD"
BASE COUNT         1979 a         1156 c         1546 g         1565 t
ORIGIN      
        1 cctatctcaa gaaactgggg gtgagggagc ttgaaggggt ttttatcagt cattttcatg
       61 aggaccacta caagggcttg ttccccataa ttgaggaact gaaaatcaag gccctgtatg
      121 ttaacaatcc tattccagac atggatctaa agaatgctgt tgagatggca aacataccgg
      181 ttttcaagat ggaaaaaggg agtaggatag atttccagga gacaagcatt gagtgtattt
      241 tttcttcaga tggagcatac gtgaacgaga acaacaattc actggttctt ttgctggaaa
      301 gcaagggcaa gaaggttatt ttcacagggg atatagaggc tgaagtggaa agtcaactac
      361 ctggatatga tatagacctt ttaaaggtgg cacatcatgg aagcaggaca tcatcatctg
      421 aggattgcat ctcaaggcta tctccagaga taagcgttat ttccgctgga agaaacaatt
      481 cctttggtca cccacatgac tcggtgttgg aaaccttggg caagtttgga tcccaggtat
      541 atcgaacaga tgaagctggt cttgtaaagg tgattataag tgatggggaa atgatggtaa
      601 ttccttatta cggccctcca tttcaggaga atctggagga attcatctac aaccatgtcc
      661 atcagttgag ctggatgctt ctttatagta tactagccta tatatcagta agaacctatg
      721 cgaaaatgga ggagaaggac catgaaatac aatgaattcc aaagcttaat agaaaatggg
      781 aatattaacc caatatacat attcactgga gatgaaacct accttctgga taaggctgtt
      841 gaatacctgg tgggattttt catgactccg gaaggaagaa ccatcaattt ggaaaacctg
      901 gatggaaaga cagctgggct ggatgacctg aaggcaagct gtgaaaccct acccttcatg
      961 tctgaaagga gggtgaccat tttaaggggg gcggaagccc tgctggatag ggattctgcg
     1021 tctgatgagg tcctgaatta tatcaagagc ctgggagacc atcaactttt gcttgtaaac
     1081 gacagggatg ggaaagtaaa gaagaattca aagctataca gataccttaa caaatcagga
     1141 agtgctgtgg tattcgagaa gctcagggga aaggaactca ctgattggat acagggaaaa
     1201 ctatcacaga ggggaaagac catgacctat gctgatctaa actacttcat tcagcaaagc
     1261 agctataaca gcaggaacat taccaccacc ctgtatgacc tggataatga aattaaaaaa
     1321 ctttgcgact tttcctccgg aaaaaacatt gaccggaata ccttggacca ggttctgatc
     1381 aggcctctcg acaagaatat ttttgacttt ctgggagctg tgggaaagaa tgatacagat
     1441 agggcaatgg aggtcttcaa cgaaatctac ctgatgaacg aaccagtgcc ccgaattcta
     1501 tttatgataa gcaggcagtt caggttgcta ttgggctatg tgacatatcg ggaaaaggga
     1561 tatgggtctg gggaaatcca ggagaaactc aaagttaagc cttatgagtt cggaaagatt
     1621 gcagcgcaag gaaatgggat ggaccagaat cggttaaggg aaatactgga ggacattcta
     1681 caaacggata ggaatctaaa aaccatatcc tccgaccaga ggttggaaat ggaaatgctg
     1741 attgtaaagc tgactgctgg ttgcaagacc tattgaaatt aatacaaaaa agaaaaaaac
     1801 ttcggtttgg ccgaagtttt attacattgc tttattaagt gccttagtca gacgactaat
     1861 ttttctagca gcagcattgt catggacgat atttttgtgg gctgctctct taagcttctt
     1921 atcaatgacc ttcaacaatg ctctcgcctc gtcgaaattt ccgttggcta cagcactctc
     1981 aaatttctta atatatgtct taatttcagt tttcttactc ttattcaagg cagttttttt
     2041 agcggttaca agaattcttt tttcagctga tttaatattt gccacgattt cacctcctct
     2101 aatctaacaa aggatattgt accatgtcat aaaccaaatt gcaagagaaa aatcactcca
     2161 cctactggta tttatgacct ccatgtagta ttattattct acttgccatt gatttaaggc
     2221 ttattaatgg tataataatc ttggatataa atcaagtact tttaataaaa tgtgcagcaa
     2281 ttctaggagg cgcagccatg agcgagatga cagaaaagag aaaagaaaaa agtgaggcga
     2341 tggagtgggt taagagcata ctggcagcca ttgcaatagc tttaataata aagacctttg
     2401 ttttcaatac aacctatgtt ctggggaact ccatgtatcc caccctacat gaacaggaca
     2461 ggttgtttgc cttgaaggtg ccattgtatt ttaagggtcc tgatagggga gatgtaatcg
     2521 ttcttgaggc acctgacagt cctggaaagg attatatcaa gagagttgtt ggaatctcag
     2581 gtgacaaggt tgaaattcag gatggcatgg tatatgtgaa tgatgttctt ctgatggaag
     2641 attacctcca aggtgaacaa tacacgcaca cctaccagga aaacatctgg attgttcccg
     2701 agggggatgt ttttgtactg ggagacaatc gtgcagaggg tgcaagcaag gacagcagat
     2761 actttggtac aatttccctt gataaggtaa agggaataac acagttcaga tactatccga
     2821 tcgatgacag attcggtaga atagaatagc ctccaccctg gaggctttat ggataaatta
     2881 gacactagga ggaataggat gcggatcaaa caggaaaata taagaaattt ttccataatt
     2941 gcccacatag accacggtaa gtcaactctt gccgacagat tgattgaaat gactggaatg
     3001 ttaacttcca gggaaatgca ggagcaggta ttggacaaga tggacctgga aagggaaaga
     3061 gggatcacaa tcaagcttaa ggcagtgagg ctggtctaca aggccaagga tggtcaggag
     3121 tacgtcttca acctgataga tactccaggt catgttgact tcaactatga ggtctcccgt
     3181 tcactggcgg cctgtgaggg tgctgttctg gtggtggatg caacccaggg agtggaggct
     3241 cagacactgg ccaatgttta cctggcaatt gaccaggatc tggaaatctt gcctgtaata
     3301 aacaagattg acctgccaag cgcaaggcca gaggaaataa tcaatgaaat agaggatatc
     3361 atcgggatag aaagtcaaga tgcccccctt atatcggcca aggacggctt gaatgtggag
     3421 gaggttcttg agcagatagt tgagaaggtg ccagcaccaa agggagaccc caatgcccca
     3481 ttaaaggcgt tgatctttga ctcatactat gacagctaca agggggtaac ctgctacata
     3541 agagttttcg aaggacaggt aaagcctgga atgatggtaa agatgatggc gacaggaaaa
     3601 agcttcgagg ttaccgaggt tggggtcaac acatccaaac acataccttt ggacagcctc
     3661 agtgccggcg aggttggata tctgactgca agtataaaga atgtcaagga agccagggta
     3721 ggagacacca taaccgatac aggcaatccc acagcaaatc ctttgccggg atacaaaaag
     3781 gtgatcccaa tggtctactg tggaatttac cctgcagagg gtgaggactt caacaccgta
     3841 agggaagctc ttgaaaagct tcaggtaaat gatgccgccc tggtatttga gcctgaaaca
     3901 tcagtggcac ttggatttgg attcaggtgt ggttttctgg gtctcttgca tatggaaata
     3961 atccaggaaa gactggaaag ggaatttgac ctaaacatta taactactgc acccagtgta
     4021 atctaccggg taaccagaac aagtggggag gtaatggaga tacagaatcc ttcaaacctg
     4081 cctgcccctg cggagattgc acacatggag gaacccattg tttctgcaaa catcatgacc
     4141 ccaacggatt acgtaggtgc catcatggag ctttgccaga ataagagggg gaccttcaag
     4201 aacatggagt atctggagga aacaagggtt gtactccact atgatcttcc actaaatgag
     4261 gtcatttacg atttctttga cgcacttaag tcaaagacca gagggtatgc atcctttgat
     4321 tacgagctta aggggtatca gcagagtgag ctggttaaga tggatattct aattaactca
     4381 gagcttgtgg atgccttctc acttattgtt catgaggaaa aggcttatga gaggggtaga
     4441 accatcgcag agagattggt ggatgtgatt ccgagacacc agtttgcagt acccatacag
     4501 gctgcaatag gcagcaagat catagccagg gagacaatta gagcccttag aaaggacgtt
     4561 ctggcaaagt gctatggtgg ggacatctct agaaagagaa agctgcttga aaaacaaaag
     4621 gaaggcaaga agaggatgag gcagataggt tcagttgatg tccctcagga ggcatttctg
     4681 accgtcttga agtatgacga gaaaaaatag attattaagg aaaagtcaca aggacttttc
     4741 ctttatttaa gtagaatgcc aggaggaaaa aatggaaaag gcaggtctgt atattcatat
     4801 accattttgc gaaagaaaat gccactattg tgatttcaca tccgtagcag gaaaccgtga
     4861 ccttattgac aggtatgtca gatatcttat caatgaaatc aaaatggttt cagcagccta
     4921 tggaaaaatc caaattgaaa cgattttcat cggagggggt actccgtctg ttctttcaga
     4981 gagcagcttg gaaacaataa tggaggccgt cgggaatggt ttttccctgg acagaaatgc
     5041 agaaatctcc atggagataa atcctggaac cctgacccac caaaaggcac ttggctacag
     5101 gaaagctgga ataaacagag ccagcgtggg gattcagtcc atgaacaaca gcctccttgg
     5161 aacaatcgga agaatccaca atgaagacca ggtcatagct accgtcaaga acctgagaag
     5221 ggcaggattt gacaacatca atggagacct gatgtttgga ctgccacacc agtcaattga
     5281 tgacttcagg gtgacccttg agaaaactgc cctgttggac ctggaacaca tatcaatgta
     5341 tggactcatc ctggaggaga acactcccct gtatggctgg catgagaggg ggcttgtctc
     5401 tgtgccgaca gaagaccagg agagggaaat gtatcacttg gggattgatt tcctcaagaa
     5461 aaaaggatac cttcaatatg aaatctcaaa ccttgccaaa aaaggccacg aatgtcgcca
     5521 caatatgggg tactggctgt taaagcccta tataggtgtg ggaatttcaa gtcattcctc
     5581 aatggagggc aggcgttatt ggaatacatc tggatttggg gagtatttcc agaagctgga
     5641 tgaagatacc cttccaattg agggggaaga gaccattacc aaggatatga tggagacaga
     5701 atatctgatt cttggaatca gattgaagga tggaatttcc gtcgatgatt atgaaaaaag
     5761 attcaaagct tcatttatgg gcaaatatgg agaggctatg gcaaagcata tggattcagg
     5821 tcttttggta ttgacagatg gaagcataag gctcactgaa aagggcatgg acctaagcaa
     5881 tcaggtggag atggatttta ttccatagag gtcaaaaagt attgacaaat acctgtaagg
     5941 gtgataattt atatatagga ttagcactcg gcaatgttga gtgctaacaa tcatccagga
     6001 gaatttagag gtgatactat gttggatgaa agaaaaatga gagttttata tgcaataata
     6061 gacagctata tatccacagc ggaacccata ggctcaagaa cgatttcaaa gggatatgac
     6121 ctgggagtca gctcagccac aatcagaaat gagatgtctg atctggagga acttggatat
     6181 ctgaacaaaa cacatacctc atctggtagg gtgccttctg acaaggcata tagactatat
     6241 gtggac
//
LOCUS       DASRQF010000071         2711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_452859, whole genome shotgun sequence.
ACCESSION   DASRQF010000071 DASRQF010000000
VERSION     DASRQF010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452859"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..141
                     /locus_tag="VFD79_01845"
     tRNA            65..141
                     /locus_tag="VFD79_01845"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:99..101,aa:Pro,seq:ggg)
     gene            complement(169..1197)
                     /locus_tag="VFD79_01850"
     CDS             complement(169..1197)
                     /locus_tag="VFD79_01850"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HZH92820.1"
                     /translation="MKTRVAVLGGGSWGTALSVLLAKNGVETGIWVRSEDQLETMLRK
                     RENPKYLPDINLPSQLGISNDIDETIKNSSIVVLAVPTHSVRGLLGQLEGKINKSQVI
                     VNVAKGIENRSMKRISEIVTEYFPENDFVALSGPSHAEEVALDIPTTVVSASRNKEVA
                     EYIQDVFSCSTFRVYTNPDVVGVELGGALKNIIALGAGISDGLGYGDNTKAALMTRGI
                     YEMSKLGVALGADPDTFSGLSGIGDLIVTCTSMHSRNRRAGILIGKGLSMEEAAEEIG
                     MVVEGIKTTKSTYELAGDIGVEMPITKELYNVLYNGANVRESVGKLMGRAMKHEMEIV
                     AKNKKTSW"
     gene            complement(1203..1790)
                     /gene="plsY"
                     /locus_tag="VFD79_01855"
     CDS             complement(1203..1790)
                     /gene="plsY"
                     /locus_tag="VFD79_01855"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HZH92821.1"
                     /translation="MEMLFVPVLAYLIGSFSSAYVVGRVFKKIDIRNHGSGNAGATNA
                     LRIMGSKLGVLTFFMDFAKGIVAVLVGTGLGGYTGGMIAALFAVLGHNWPIFFKFKGG
                     KGIATTIAAMAMLQFPITLLSVVVGVLTAAISKYVSLGSLVFLTVLFALSFTGMSDGG
                     SIVSIITFVLMVLGYYRHKGNIKRLIEGTENKIGR"
     gene            complement(1793..>2711)
                     /gene="der"
                     /locus_tag="VFD79_01860"
     CDS             complement(1793..>2711)
                     /gene="der"
                     /locus_tag="VFD79_01860"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HZH92822.1"
                     /translation="FYELGLGEPMVVSAEQSLGLGDLLDELAKYFPDHKDTEANEDTI
                     RVAFIGRPNAGKSSLINHILGEERVIVTDIPGTTRDSIDSHFTVGENDYIFVDTAGLR
                     RKRYISEKVERYSVVRTLTAIDRADICVMVIDATMGPTEQDTKIAGYAHDNGKAIILA
                     VNKWDIVDKNDKTYLSFEKDLRETFGFLNYAPILFISAKTGQRVDKLLETLNIVNNNY
                     NMRISTGVLNDILNQAVLMNQPPSDKGRRARIYYGTQVSVRPPRFVIFVNSKELMHFS
                     YVRYLENQIRNHFGFMGTPIKFELREKGE"
BASE COUNT          721 a          667 c          482 g          841 t
ORIGIN      
        1 taattccaaa tcattttaaa ccactttaca aaatgaaatt tatcagttat aatactgctt
       61 gtggcggggt gtggcgcagt ttggtagcgc actagtctgg gggactaggg gccgtaggtt
      121 caaatcctat cactccgacc aaataaatga gctggtatac ccaagaggtt accagctcgt
      181 ttttttattt tttgccacta tttccatttc atgcttcatg gcccttccca taagcttgcc
      241 taccgactcc cttacattgg ctccattgta gagcacatta tagagctctt ttgtgattgg
      301 catctccact ccaatatctc cagccaattc atatgttgac ttggtcgtct ttattccctc
      361 aacaaccatt cctatttctt cagctgcctc ttccattgag agaccctttc caattaggat
      421 gcctgccctt ctgtttctgc tgtgcatact ggtacaggtt acaataagat ctccaattcc
      481 tgatagtcct gagaaggtat cgggatcagc tcccagtgca actcccaatt tagacatttc
      541 atagattccc ctggtcataa gagctgcctt ggtattgtct ccatagccaa ggccatctga
      601 tattccagct cccaaagcta ttatgttttt cagtgcacca ccaagctcta ctccaactac
      661 atcgggattt gtatatactc taaaggtgga gcatgaaaag acatcttgaa tatactctgc
      721 aacctccttg tttctggatg cggataccac agtagttggt atgtccagag ccacctcttc
      781 tgcgtgggat ggtccagata gggcaacaaa gtcattttct ggaaaatatt ccgtaacaat
      841 ctctgaaatt ctcttcattg atctgttttc aataccctta gctacattta ctattacctg
      901 tgacttgtta attttacctt ccagctgacc gagcaatcct cttacactat gggttggaac
      961 tgcaagcaca acaatactac tgttcttgat tgtttcatca atatcattgc ttattccaag
     1021 ctgtgatggc agattaatat caggtaaata tttggggttt tccctctttc taagcattgt
     1081 ctccaactgg tcctcggacc tgacccaaat tccagtttca acaccgttct ttgcaagcag
     1141 aactgaaagg gctgttcccc agctgcctcc acctaaaact gctactctgg ttttcaataa
     1201 aatcacctgc ctattttatt ctcagttcct tcaatcaatc ttttaatgtt ccctttatgc
     1261 ctatagtacc ccagaaccat caatacaaat gtaatgatgg atacaatgct tcccccatca
     1321 gacattccag taaagctaag cgcaaacaaa accgttagaa aaaccaggga tcccagtgaa
     1381 acatacttgc ttatggctgc agtcaataca cccacaacaa cacttaagag agtgatggga
     1441 aactgtagca tggccatcgc tgctattgtg gtggcgatac cctttccacc tttgaattta
     1501 aagaagattg gccaattgtg ccccaatacg gcaaacaagg ctgctatcat tcctccagtg
     1561 tagccaccga gtcccgtgcc gaccaaaact gcaacaattc ccttggcgaa atccatgaaa
     1621 aaggtaagca caccaagctt gctgcccata attcttagtg cattggtggc gcctgcattt
     1681 ccgcttccat ggtttcttat atcgattttc ttgaaaaccc ttccaacgac ataggctgaa
     1741 gaaaagcttc ctatcaggta tgcaagaacc ggtacaaaaa gcatctccat aactattctc
     1801 ccttttctct caattcaaac ttaataggtg tacccatgaa accaaagtga tttctaatct
     1861 ggttttcaag atacctaaca tatgaaaagt gcatcagttc ctttgagttt acaaatataa
     1921 caaatcttgg tggtctcaca gatacctggg ttccatagta tatcctggcc cttcttccct
     1981 tgtcagacgg tggctgattc ataagaactg cctggtttag aatgtcgttt agaactcctg
     2041 tgctgattct catattgtaa ttgttgttta caatattcaa tgtttcaagg agcttgtcca
     2101 ccctttgtcc agtcttagcc gaaatgaaga gtataggtgc gtagtttagg aacccaaatg
     2161 tctcccttag gtccttttca aatgaaaggt aggtcttgtc attcttgtca acaatatccc
     2221 atttgtttac agcgagaatt atggccttgc cattatcatg ggcatagcct gctatttttg
     2281 tatcctgttc tgttggtccc attgtggcgt ctattaccat gacacatata tcggctctgt
     2341 ctattgcagt caaagtcctc actacagagt atctttccac cttctcggag atgtaacgtt
     2401 ttctccttaa gccagctgta tcaacaaaaa tataatcatt ctctccaacg gtaaagtgac
     2461 tgtcgattga atcgcgggtt gtacctggaa tatcagtaac aataaccctt tcttcaccaa
     2521 ggatatgatt tattagagaa gattttccag cgttgggtct tccaatgaag gccaccctga
     2581 tggtatcttc atttgcctct gtatccttgt ggtctggaaa atacttagcc aactcatcca
     2641 aaagatcacc cagtcccagt gactgttcag ctgataccac catgggctca cctaaaccca
     2701 gttcataaaa c
//
LOCUS       DASRQF010000072         2597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1074106, whole genome shotgun sequence.
ACCESSION   DASRQF010000072 DASRQF010000000
VERSION     DASRQF010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074106"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VFD79_01865"
     CDS             complement(<1..766)
                     /locus_tag="VFD79_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZH92823.1"
                     /translation="MDRQQQAIQTINMNLAVSAGAGTGKTKVLTERYVHILENGDLDE
                     GREIESLVAITFTKKASTEMKERIEQEISSRLDHGEKWRRLYRDFSKAYIMTIHSFCG
                     KLLRENPVQADIDPGFAVLEEYVADRLLREAAARVLDRVLREEKNLQEVSVLGKKDIG
                     PLTDELIDIYKKIRTLGMSMEEAMDKTVTHISSMEIDPNMLREIRETALYLGGEMGRG
                     KFVSILKEPVVQDFLNGEEDNQLRLALQLLGDNMGTS"
     gene            complement(766..>2597)
                     /locus_tag="VFD79_01870"
     CDS             complement(766..>2597)
                     /locus_tag="VFD79_01870"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HZH92824.1"
                     /translation="TLEKIQQLIKDVEAISLDSDNGESIAGHGRFALDLLDSMKLDRY
                     VMDRSSLVDDWQWTIRELNALNAFREILEDITQMAYIDWELSFKDFMDILRDYVVQKE
                     IIIQNPATNGIELLNPTNGRGLIGDHKFIIGLNKSQYPSLDENNFLFAQKSLERLKRG
                     GIRHLDYREKFENELLKISSIIGSTREALYLGTSSSLGGEDDLPSIFLARISRTAGKR
                     DGKSNLVQEEISHESFIGTSLDQVSSRDDFNRLALWRYYQDKLEEADMEYFLQNSREL
                     KDHISKRLHSQYKRSGGDLDKYSGVLDSMDVKTIVEGKTRDRVYSVSYIEDYIKCPYA
                     FLLARIFNLQETPREWQETKPMDRGQIFHKVLCTYYRAYRNDLEKDPLAFSIDSTREH
                     LRSLVISEAREYGLGNVSGGDRLFILSMEETLVGYISADIERMRRSKVKVYPYLMEKE
                     FGENRSFKISKDGTEIHFKGIIDRIDKVEDGSQILIDYKSSSYGKRTREDIENGLSIQ
                     LPMYIISQDEDTITGGYYGIISSGDLYPALAIKDKAPFVGGRNTGAVDEDEFRRLLQI
                     AREAVFKAVEGIRDGYFPVAPKECSAYCIFRDICRYGDKEEMN"
BASE COUNT          598 a          706 c          481 g          812 t
ORIGIN      
        1 tgctcgtgcc catgttgtct ccaagaagct gcagggccaa cctaagctga ttgtcttcct
       61 caccatttaa gaagtcttga actactggct ccttaagaat acttacaaac ttgccccttc
      121 ccatttcacc gcccaggtac agggcagttt ctcgtatttc acgaagcata ttcgggtcaa
      181 tctccatact tgaaatatgg gtgacagtct tgtccattgc ctcttccata ctcattccca
      241 gcgtcctgat tttcttgtag atatcaataa gttcatccgt tagtggtcca atatccttct
      301 ttccaaggac gcttacctct tgcaggttct tttcttccct caaaactctg tcaagcaccc
      361 tagctgctgc ctcccttaaa agcctgtctg caacatattc ctccaggact gcaaagcccg
      421 ggtctatatc tgcctggacg ggattttccc ttaggagttt cccgcaaaag ctatgtattg
      481 tcataatata tgcctttgaa aagtccctgt acagcctcct ccatttttca ccatggtcca
      541 gtcttgatga aatttcctgc tctattcgct ccttcatttc agtgcttgcc ttcttggtga
      601 aggttatggc caccagggac tctatctccc tgccttcatc caggtctccg ttttcaagaa
      661 tatggacata cctttcagtg aggactttag tttttcccgt ccccgcacct gctgaaactg
      721 caaggttcat gttgatggtc tgaattgcct gctgctgtct gtccatcaat tcatctcctc
      781 cttgtcaccg tacctgcata tgtccctgaa tatgcagtaa gctgaacatt ccttgggggc
      841 tacgggaaag tatccgtccc ttattccctc aaccgcttta aatacagcct ctctggctat
      901 ttgaagcagc ctcctgaatt catcctcatc cactgcccct gtgttcctgc cacctacaaa
      961 gggcgccttg tccttgattg ccaaagccgg gtagaggtca cctgaagaaa ttatcccata
     1021 gtagccgcct gttatggtgt cttcatcctg ggaaattata tacatgggaa gctgtatgct
     1081 caatccattt tcaatatcct ccctggtcct cttgccatag gaggaggact tatagtcaat
     1141 caggatttgg cttccatcct ctaccttgtc aatccggtca attataccct tgaaatggat
     1201 ttcagttcca tccttactaa tcttaaagct cctattctca ccaaattcct tctccataag
     1261 gtatggatac accttaacct tggatcttct catcctctca atatctgctg aaatatatcc
     1321 caccagggtc tcctccattg acaggataaa caatctgtcc ccaccagata cattgcccaa
     1381 tccatactcc cttgcctcac ttattacaag ggaccttaga tgttccctgg ttgagtcgat
     1441 ggagaaggcc aaaggatcct tctcaaggtc attcctgtag gccctgtagt atgtgcatag
     1501 caccttgtgg aatatctgtc ccctgtccat tggcttggtc tcctgccatt cccttggggt
     1561 ttcctgaagg ttgaaaatcc tggccaggag gaatgcgtag gggcacttga tataatcctc
     1621 tatgtaggag actgaataga ccctgtccct ggttttccct tcaacaatgg tctttacatc
     1681 catggaatcc agcactccgc tgtacttgtc caggtctcct cctgaacgtt tatactggga
     1741 atgcagcctt ttactgatat ggtccttaag ctccctgctg ttttggagaa agtattccat
     1801 gtcagcctcc tccagcttgt cctggtagta tctccaaagt gccagcctgt tgaagtcatc
     1861 cctactggat acctgatcaa gacttgtgcc tataaaggac tcatggctga tttcctcctg
     1921 aacaagattg gatttcccgt cccttttgcc agcggttctt gaaattcttg cgagaaaaat
     1981 ggatggaagg tcatcctccc ctccaaggga tgatgaggtc cccagataca gggcttctct
     2041 tgttgaccca attatacttg atattttcaa cagctcattc tcaaatttct ccctgtagtc
     2101 caaatgcctg attccacccc ttttaagcct ttcaagactt ttttgtgcaa agaggaaatt
     2161 gttctcatca agacttggat actggctctt atttagtcca atgataaact tatggtcccc
     2221 aatcagtccc cttccatttg tgggatttaa aagttcaata ccgtttgttg cagggttttg
     2281 gattatgatc tccttttgga caacataatc ccttaggata tccatgaaat ccttgaagga
     2341 aagctcccag tcaatatagg ccatctgggt aatatcctcc agtatttccc tgaaggcatt
     2401 cagggcatta agctccctta ttgtccattg ccaatcatcc acaagactgg acctgtccat
     2461 tacataccta tccagcttca tggaatccaa taggtccagg gcaaaccgtc catgacctgc
     2521 tattgattcc ccattatctg aatccaggct tattgcttcc acatccttta tcagctgctg
     2581 aatcttttca agagtat
//
LOCUS       DASRQF010000073         2912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_203664, whole genome shotgun sequence.
ACCESSION   DASRQF010000073 DASRQF010000000
VERSION     DASRQF010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203664"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VFD79_01875"
     CDS             <1..253
                     /locus_tag="VFD79_01875"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HZH92825.1"
                     /translation="KDMEFHRYLWNKSGSKRRVQILEQIFFQLRIAMLYDTNETGDLS
                     KTATDHYEIINALKGRDISKCKEALQKHIITYKEGLVQD"
     gene            332..1510
                     /locus_tag="VFD79_01880"
     CDS             332..1510
                     /locus_tag="VFD79_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HZH92826.1"
                     /translation="MEKILAFSVQEYRQRLEKTKRSMEEKGIEVLIISDPANMNYITG
                     FDGWSFYVHQCVIVMIDQDEPLWIGRGQDSNAARLTSYLGEKSIYPYTDDYVQSLIKH
                     PMDFVADILKEKGYDKNTVGTEMDTYYYTAKCQESLESNLPNCKFIDATSLVNWVKLI
                     KSDMEIDYIKKAAVIAEKTMDAAFKSIHVGTRQNEAAANVYHAQIYGTEEYGGDYTSI
                     VPLMPAGIRTSTPHLSWTDEPYKDGDTVLVEIAGNYRRYHCPLSRTFILGSAPQKVKD
                     LASVVVQGIEDTLAVVKPGITCEEIERTWAKSIAKSGFIKDSRVGYSFGLNYPPDWGE
                     HTVSLRPGDKTILEPNMTLHFMPGIWLDDCGVDISEPIRITDSGVELLTNYPRRLLEK
                     "
     gene            1576..>2912
                     /locus_tag="VFD79_01885"
     CDS             1576..>2912
                     /locus_tag="VFD79_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HZH92827.1"
                     /translation="MYNKFPDGNILNVNLNGGVVNKEVLPGDVYRLYPGGSALGLYLA
                     MKDMKPGIDPLGEDNNLVFSVSALTGLPISGISRVNVTAKSPLTGGMGDSQAGGFFPV
                     HMKANGLDAIVLKGKSDKPVYLFIDGEDVQLKDASALWGKVTGEAEKIIREELGRDDV
                     EIAQIGPGGENLVNYACIINMCNRANGRNGMGAVMGSKNLKAIVVAKGKSQKPVDSEG
                     FFGLAKSVMARLEENGSVAGLGKYGTDGDLEGFSEEGFLPTNNWTTGYFPQGAKNITG
                     ETMYDTILKERDTCYSCAVRCKRVVEVPGMVDPLYGGPEYETAATFGSYCGVTSLEYV
                     SLANQMCNMYGLDTISCGATIAFAMEAYEKGLIDKTVTDGIDLKFGNGEALPVLIEKI
                     ANKEGFGRLLALGSYRYAQTLGEEALDLTVTVKKQELPAHMPQYKPAVGLVYAV"
BASE COUNT          933 a          535 c          751 g          693 t
ORIGIN      
        1 taaggatatg gagtttcaca gatacctgtg gaataaatcc ggaagcaaga gaagggtgca
       61 gatacttgaa cagatatttt tccaattgag gattgccatg ctctacgata caaatgaaac
      121 gggagatctt tcaaagacag ccacagacca ctatgaaatt ataaacgctt tgaagggtag
      181 ggacatttca aaatgcaaag aagcattgca gaagcatatc ataacctata aggagggctt
      241 ggttcaggat taggtgctca aagtatttgg gagcattatt taatacacca cataatgtcg
      301 acaatagaca atcaacaaaa ggaggaaggc catggaaaaa atattagcat tttcagtcca
      361 ggagtacagg cagagacttg agaaaacaaa acgaagcatg gaagagaagg gaattgaggt
      421 tctgatcata agcgatccgg caaatatgaa ctacataaca ggctttgacg gttggtcctt
      481 ttatgttcat cagtgcgtca tagtcatgat tgaccaggat gagccccttt ggattggcag
      541 gggacaggac tcaaatgccg ccagactgac atcataccta ggcgaaaaga gcatctatcc
      601 ctacacggat gattatgtcc agtcccttat aaagcatccc atggatttcg tagctgacat
      661 cctaaaggaa aagggatacg ataagaatac agtaggtaca gagatggata cctattatta
      721 tacagccaag tgtcaagaga gtcttgagag caatctacca aactgtaagt ttattgatgc
      781 aacaagtttg gtaaattggg tcaagcttat taagtcagac atggagattg actacatcaa
      841 gaaggcagca gtgattgccg aaaaaacaat ggatgcagca tttaaatcca tccatgttgg
      901 aactagacag aacgaggccg cagcaaatgt ataccatgct caaatatatg gaactgaaga
      961 gtatggggga gactacactt caattgtacc tctgatgcct gcaggtataa ggacatctac
     1021 tcctcattta agctggaccg acgagccata caaggacgga gatacggtac ttgttgaaat
     1081 cgcaggaaat tacaggagat accactgtcc tctttcaagg acctttatcc tcggcagtgc
     1141 acctcagaag gtaaaggatt tggcctcggt ggtcgttcag ggtatagagg acacccttgc
     1201 agttgtgaaa ccaggaatca catgtgaaga aattgagcgg acatgggcta agagtatagc
     1261 caagagcggc tttatcaagg attcaagggt tggctactcc tttggactaa actacccacc
     1321 tgactggggt gagcatacag tcagtttaag gccaggtgac aagacaatcc ttgagccaaa
     1381 tatgacacta cactttatgc ctggaatttg gcttgatgat tgcggagtgg atataagtga
     1441 gcctattaga ataactgata gtggagtaga gcttcttacc aactatccaa gaaggctcct
     1501 tgaaaaataa gggtttgcca tagtggttgg atgagacaga ggaagttaag caattaatag
     1561 ttaggaggct aaaatatgta caacaaattt ccggacggta atatattgaa tgttaactta
     1621 aatggaggag tggtcaataa ggaagtcttg ccaggtgatg tatacagatt gtaccctgga
     1681 gggtctgcat tgggtttgta tctggccatg aaggatatga aaccagggat cgatcctttg
     1741 ggagaggaca ataatctggt attttcagta tcagcattga caggacttcc aataagcgga
     1801 ataagcagag ttaatgtaac tgccaaaagc cctttgacag gtggaatggg tgacagtcag
     1861 gcaggtggct ttttcccggt acatatgaag gcaaatggtc tagatgccat tgtacttaag
     1921 gggaaatcag acaagccggt atacctattt attgatgggg aagatgtgca actgaaagac
     1981 gcatcagccc tttggggaaa ggttacagga gaggctgaga agataatcag agaagagctt
     2041 ggaagagatg atgttgaaat cgcccagatt ggacctggag gagaaaatct tgtaaattat
     2101 gcatgcatca taaacatgtg caatagggcc aatggtagaa atggtatggg agcagtaatg
     2161 ggctcgaaaa atttgaaggc cattgtagtt gccaagggca agtcccagaa accagtagac
     2221 agcgaaggct tctttggcct tgcaaagagc gtcatggcaa gactggagga gaacggatct
     2281 gtagctggac ttggcaaata tggaacagat ggtgaccttg agggcttcag tgaagaggga
     2341 tttctaccta caaacaactg gacaacagga tattttcctc aaggagccaa aaatataacc
     2401 ggtgagacaa tgtatgacac aattctcaag gaaagggaca cctgctattc ctgcgcagta
     2461 agatgcaaga gggttgtgga agtgcccggt atggtcgatc ccctgtacgg cggacctgag
     2521 tatgagactg cagctacatt cggttcatat tgtggggtta caagccttga atatgtatcc
     2581 ctggccaatc aaatgtgtaa catgtacggt cttgacacca tctcatgcgg agcaaccata
     2641 gcctttgcca tggaggcata tgagaagggc cttattgaca agactgtgac cgatgggata
     2701 gacctaaagt ttggtaacgg tgaagctctt cctgtactga tagagaaaat tgcaaacaag
     2761 gagggttttg gcaggctctt ggctctggga agctataggt acgcacaaac tcttggcgaa
     2821 gaagccctag acctaacggt aacagtaaaa aaacaggaac ttcctgccca catgccacag
     2881 tataagccag cggtaggcct agtatatgcg gt
//
LOCUS       DASRQF010000074         4265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_962186, whole genome shotgun sequence.
ACCESSION   DASRQF010000074 DASRQF010000000
VERSION     DASRQF010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962186"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VFD79_01890"
     CDS             complement(<1..749)
                     /locus_tag="VFD79_01890"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HZH92828.1"
                     /translation="MKILLVRPKPHKETIGLKHVMVCEPLELEYLVSNIPEDIKGRCD
                     VEIVDMILEKRSFRSIIQEKRPHMVLFTGYITHVGTISDMAEKVKSILPAAITGVGGV
                     HAEVVAEDYSSPDLDLVFGRNGVKAFAGALRGILEGKDLDEIREEIKSISREELDFNL
                     RPPDRSSVDRYRSSYYYMFHNPCALIKTSYGCPYTCRFCFCREITGGRYHSRSMDDVM
                     DELEEIPEEEVYIVDDDFLFGRDRLLSFARLL"
     gene            complement(749..1135)
                     /locus_tag="VFD79_01895"
     CDS             complement(749..1135)
                     /locus_tag="VFD79_01895"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZH92829.1"
                     /translation="MLEISKIKNPKAKHLEMAAEDLEGLDQKFDVVTCTHSLPYYRNK
                     KRVMAKIAERLVPGGKLIIAFASGNSPYDRVALSLVKLTTGPAAYPSDMDFRDMIREV
                     FAVESRHVIREAIYMPTIAVYTLRRL"
     gene            complement(1114..1362)
                     /locus_tag="VFD79_01900"
     CDS             complement(1114..1362)
                     /locus_tag="VFD79_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92830.1"
                     /translation="MKKAIWDFWAGRYDRLWVQKKSLKPTREYVRGIIREEWMEGARS
                     LLDLGCGPGELISSLGEDLRDMDITGLIFQGPCWRFQK"
     gene            complement(1367..2641)
                     /locus_tag="VFD79_01905"
     CDS             complement(1367..2641)
                     /locus_tag="VFD79_01905"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZH92831.1"
                     /translation="MLIYLEKVRRKTLFSRFEPIVTEPLELLYIKSVLDSMGIENGII
                     DPLFSLEASGGDLPDMVILTGYNVAEEEMKKRAHWWKNQNRDILVMVSGVHVQLNSGK
                     FMEDYIDFIFHTQDFGVFKELLYRIQREEEVNGLTGLHTRKGDGSWELGEERPVQSPE
                     AILPSRDLYKSNKDRTRYVDKKGLALVKGGRGCPYACSYCYCRSLNGGTFVRPDFSRM
                     FEEMETIGADHYWIVDDVLLVDSKDAMEFIRASRDVSFEGGIIAYLKADFIVREREFL
                     KDLRDGGLSEVIIGFETPDREELLDYNKKVDVYIYEEAISLLKDNNIQLTALFMVHPD
                     YGLGEFWRLWTFILKNRLDLYTISIFTPLPGSDEFLSYQDRLTTDKAESFDFLHLVLP
                     SRLPRPVFYGLFYLSHLKLLFSKRILKYILMR"
     gene            complement(2625..3569)
                     /locus_tag="VFD79_01910"
     CDS             complement(2625..3569)
                     /locus_tag="VFD79_01910"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZH92832.1"
                     /translation="MGIFEFNEKEGLKLDRDILSLLTILERANPCKQDYHIEKGTFIV
                     SYRLRMDLLNLWGRLSVRINTNVVGLPISVADPGYLGNEDQVEGFLGKQRGLWVVLNG
                     ERPFSVPAKTLSTFVFRNRFKSFSEYINALRSPYRRRIRRALDKGKDLRVQKADFTED
                     HHRLYMDVIGRSDHPLEILPMEFFRDYHGDIYEFRSKEGRLLAFVQTERNKDRLRFLF
                     CGFRREDVEEFDLYYNMLLWIVRLGIEEGVSEIDFGQTSEESKLKIGCQERERFLYIH
                     HNNPVLRWILRRLLPLFSYRPYPRSHRVFKEEDHADIS"
     gene            complement(3569..>4265)
                     /locus_tag="VFD79_01915"
     CDS             complement(3569..>4265)
                     /locus_tag="VFD79_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92833.1"
                     /translation="LDGYHRPQSFGMAHNPPHIHRYFEEHGFDRIQMSTYMKDMDSLE
                     PLVPARMKKRLKERYTVRPVDFSRLREEAATYTRINNDAFRDHLFYYRRVPEEDLELF
                     RDFRFLLKPENLLFVQRDGVDVGFMLWYPDFHQIMDKKSGIGLRTVLKARLHPEKIDT
                     FKIVEIGVVKEEQGKGAILALFDHCQELTEGRYKHFESGWVLKDNPRSKALGDKWSDG
                     ISKTFAAYVKELV"
BASE COUNT         1065 a         1131 c          827 g         1242 t
ORIGIN      
        1 aggagtctgg caaagctcag aagcctgtcc cttccaaaga ggaagtcatc atccacaata
       61 tagacctctt cctccggtat ttcctcaagc tcatccatta catcatccat tgaccttgaa
      121 tggtatcttc cacctgtaat ctccctgcag aagcaaaacc tgcaggtgta gggacatcca
      181 taggatgttt ttatcaaggc gcatgggttg tggaacatat agtagtagct gctgcgatac
      241 ctgtctacgc tacttctatc tggaggtctt agattgaaat caagctcttc cctagagatg
      301 gactttatct cttctcttat ttcatcaaga tccttaccct ccaggattcc cctgagggca
      361 cctgcaaatg cctttactcc attccgtcca aatacaaggt caaggtccgg gcttgaataa
      421 tcctccgcca caacctctgc atgcacaccg cctacacctg ttattgctgc aggaaggatt
      481 gactttacct tctctgccat atcacttatt gtccccacat gggtgatgta gcctgtaaaa
      541 aggaccatgt ggggtctttt ttcttgaatt atgctcctga aggatctctt ttcaagaatc
      601 atatccacta tttcaacatc acatctgccc ttgatgtcct ctggtatgtt tgaaaccagg
      661 tattccagct caagaggctc gcaaaccatg acatgcttaa gtccaattgt ttccttgtgg
      721 ggctttggtc ttaccaagag tatcttcatc acagcctcct tagtgtgtag acggcaattg
      781 tgggcatata gattgcctcc ctgattacat gtctgctttc aacagcgaac acttccctaa
      841 tcatatccct aaaatccata tccgaagggt aggctgcagg accggttgtc agctttacaa
      901 gggacagggc taccctgtca tatggactgt ttccactggc aaaggcaatg ataagctttc
      961 cccctggcac cagtctctct gcaatcttgg ccattaccct tttcttgttc ctgtagtagg
     1021 gcagggaatg ggtacaggtc accacgtcaa atttttggtc caggccctcc aggtcctctg
     1081 cagccatttc caggtgtttg gccttaggat tctttatttt tgaaatctcc agcatggccc
     1141 ttgaaaaatc aaccctgtta tgtccatatc ccttaggtcc tcaccaaggg aacttatgag
     1201 ctcaccagga ccacatccaa ggtccagtag gcttcttgca ccctccatcc attcctccct
     1261 gatgataccc cttacatatt ctcttgtggg tttaagagat ttcttctgaa cccagagcct
     1321 gtcatatctc cctgcccaaa aatcccatat tgcttttttc atatgtttac ctcatcaaaa
     1381 tatattttaa tatccgtttt gaaaagagaa gcttcagatg actcaggtag aacaaaccat
     1441 agaatacagg tcttggaagt ctggatggca gaacaagatg taaaaagtca aaggactctg
     1501 ccttgtctgt agtcaatctg tcctgatagc ttaagaattc atcactcccc ggaagtggtg
     1561 tgaatattga aattgtgtaa aggtctagcc tgtttttaag gataaaggtc cacagcctcc
     1621 aaaattcccc caggccatag tccggatgaa ccatgaagag ggcggtcagt tgtatgttgt
     1681 tatcctttag cagactgatg gcctcctcat agatatacac gtccaccttc ttgttgtagt
     1741 caagcaactc ctccctgtca ggggtttcaa agcctattat aacctcagaa agtcccccat
     1801 cacgcaggtc ctttagaaac tccctctccc ttactatgaa gtcagccttc agataggcaa
     1861 tgatccctcc ctcaaagcta acatcccttg aagccctgat aaattccatg gcatcctttg
     1921 agtccacaag aagtacatcg tccacaatcc agtaatggtc tgcccctatc gtctccattt
     1981 cctcaaacat ccgggaaaaa tccggcctta caaaggtgcc gccatttagg ctcctgcaat
     2041 agcaatagga acaggcatag ggacaccccc ttcctccctt gacaagggca agccccttct
     2101 tgtcaacata acgggtccga tccttgttgc tcttgtaaag gtctctggat ggaaggatag
     2161 cttccggact ttgcacaggt ctctcctctc ccagctccca gctgccgtcc cctttccttg
     2221 tatgtagtcc agtaagtcca ttgacctcct cttccctttg aatacggtat aaaagctcct
     2281 taaagacccc gaagtcctgg gtatgaaata tgaaatctat gtagtcttcc atgaattttc
     2341 cgctgtttag ctgtacatgg acccctgata ccataacaag tatatcccta ttctgattct
     2401 tccaccaatg ggcccttttc ttcatttcct cttctgccac attatagcct gtaaggatta
     2461 ccatatcggg gaggtctcct ccagaagcct ctaggctgaa aagggggtca ataattccat
     2521 tttcaatccc cattgaatca agtacagact ttatgtacag gagctccagg ggctcggtga
     2581 ctataggctc aaaacgtgag aatagggttt ttcttcttac cttttcaaga tatatcagca
     2641 tggtcttcct ccttgaaaac cctgtggctc cttggatatg gtctgtatga aaacagcggg
     2701 aggagccttc ttaaaatcca ccttaggact gggttgttat gatggatgta gagaaacctt
     2761 tccctctcct gacatccaat cttaagcttg ctctcctctg aggtctggcc aaagtcaatt
     2821 tcacttacac cctcctctat tccaagcctt actatccaaa gaagcatatt gtaatacagg
     2881 tcaaactcct caacatcctc tcttctgaag ccacagaaaa gaaatctcag cctgtcctta
     2941 ttcctctcag tctggacaaa tgccagaagt ctaccttcct ttgacctgaa ctcatatatg
     3001 tccccatgat agtccctgaa aaattccatg ggcaagatct caagaggatg gtctgacctt
     3061 ccaatcacat ccatgtacag cctatgatgg tcctcagtaa agtccgcctt ctggaccctt
     3121 aggtcctttc ccttatccag ggcccttctg atccttcttc tgtagggact cctcagtgca
     3181 ttgatatatt ctgaaaaaga tttgaacctg ttcctaaaga caaatgttga aagagttttt
     3241 gcaggaaccg aaaagggtct ttccccatta agcaccaccc aaagccctct ttgtttacca
     3301 agaaagcctt caacttggtc ttcattgccc agataacctg ggtcagccac ggatattggc
     3361 agacctacaa catttgtatt tatccttact gaaagtcttc cccaaaggtt tagaaggtcc
     3421 atccggagcc tataggagac aatgaaggtc cctttttcaa tatggtagtc ttgcttgcag
     3481 ggattggccc tttcaaggat ggttaggaga ctcaggatat ccctatcaag ttttagccct
     3541 tctttttcat tgaactcaaa aattcccact aaaccagctc cttgacatat gcggcaaagg
     3601 tcttggatat tccatcggac cacttgtctc ccagggcctt tgatcttggg ttgtccttca
     3661 ggacccagcc ggactcaaaa tgcttgtacc ttccttccgt tagctcctga caatggtcaa
     3721 agagggccag gattgctccc ttgccctgtt cttccttgac aacccctatc tcaacaatct
     3781 tgaaggtgtc tatcttttct ggatgcaacc ttgccttgag gacagtcctc aaaccaatcc
     3841 cagatttttt atccattatc tgatgaaaat caggatacca gagcataaat cccacatcca
     3901 ccccgtctct ctggacaaag agcaggtttt ctggcttcag aagaaatctg aagtccctga
     3961 acagctccaa atcctcctcc ggaaccctcc tgtagtagaa aaggtggtcc ctgaaggcat
     4021 cgttgttgat tctagtgtat gtggctgcct cctccctgag tcttgaaaaa tcaacaggac
     4081 ggactgtgta tctttcctta agtcttttct tcattctggc aggtacaagg ggttccaggc
     4141 tatccatgtc cttcatgtat gttgacatct ggatcctgtc aaatccgtgt tcttcaaaat
     4201 acctgtgtat gtgaggcggg ttgtgggcca tgccaaagct ttgaggcctg tggtagccgt
     4261 caagc
//
LOCUS       DASRQF010000075         3791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_81084, whole genome shotgun sequence.
ACCESSION   DASRQF010000075 DASRQF010000000
VERSION     DASRQF010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81084"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VFD79_01920"
     CDS             complement(<1..688)
                     /locus_tag="VFD79_01920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZH92834.1"
                     /translation="MASAAYEKKREYLLSNDNLYKTSLYLAWPVIIQLLLQVLVGTID
                     MKMVGNVGVDAISAVGTSRNIIMILMVLVIAISTGTTAMVARFVGKDDHEGASVAAGQ
                     AFMLSLVLSAFIVPVGLLTNEFSLKILGVTDGVLNYAIEYMEVFFLAVPFFLLHFMAR
                     SIFQGAGDTKTPLYIDIMMNIINVIGNYIFIFGMFGFPAYGVAGAAMGTALSRVISVM
                     VAWGALLSGKF"
     gene            complement(844..1629)
                     /locus_tag="VFD79_01925"
     CDS             complement(844..1629)
                     /locus_tag="VFD79_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HZH92835.1"
                     /translation="MLYRELAPISNEAWGEIDERASEVLKSYLSARKVVKVNGPKGLS
                     FNVISEGRLGDTEEDGDVCYANYKVLPLTESRIEFEMDRWELDNVSRGAKDVDYGPLE
                     DALEKIALFEENAVYNGLENAGIVGLDGAAEGKAIKLGDDAKSLMESITEGVLRLRRN
                     FAGGSYTLVVGKDAYKRIIAQDSGYPLKKRIESLIEGKIILSHVVEGAYLIPYDHDDL
                     ELTIGRDFSIGYQEHDAEKVKFFVAETFAFRVLDPALIVKFSL"
     gene            complement(1643..1987)
                     /locus_tag="VFD79_01930"
     CDS             complement(1643..1987)
                     /locus_tag="VFD79_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZH92836.1"
                     /translation="MTQYHEPVERLDDKTMDITRALVSLKEEIEAVDWYNQRVAAATD
                     PALKEILAHNRDEEIEHAVMTLEWLRRNMGAWDEEMKTYLFTEGDISHLEEGGEGEEP
                     SGDKGLNIGDLK"
     gene            complement(2054..2920)
                     /locus_tag="VFD79_01935"
     CDS             complement(2054..2920)
                     /locus_tag="VFD79_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZH92837.1"
                     /translation="MKYEGTVYRPPSEARSWILQATIGCSHNRCTFCSMYKDKSFRIR
                     KTEEIIQEIHEARRLYGNLKRIFIADGDALMIRTEDLVLIIGELKNTFPECERIGIYA
                     SPKSIHLKSDADLKTLKEAGLGIAYLGLESGSDDILKAVKKGDTRGGIISAGRKIKEA
                     GLKLSVTVISGLGGRENWEDHARETARAVNMIRPDYLGLLTLMTEPGTELWDQVESGE
                     FSLLSPYEIAQETLLMLSQLDSPGCVFRSNHASNYVALKGTLNRDREEMIKTLETALA
                     DGDFKAEWTRRL"
     gene            complement(2993..3445)
                     /gene="bcp"
                     /locus_tag="VFD79_01940"
     CDS             complement(2993..3445)
                     /gene="bcp"
                     /locus_tag="VFD79_01940"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(3329..3331),aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HZH92838.1"
                     /translation="MSEYDFTLKETDGKEISLKDFKGRNVVVYFYPKDNTSGUITEAV
                     GFRDLNQEFEELETVIMGISKDSLSSHKKFRDKQELNFLLLSDEEGKVHEQFKVIVPK
                     KMYGKEYMGTERSTFIFNRDGQMVKEYRKVKSAGHAEEVLDYIKENMK"
     gene            complement(3504..>3791)
                     /locus_tag="VFD79_01945"
     CDS             complement(3504..>3791)
                     /locus_tag="VFD79_01945"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZH92839.1"
                     /translation="ENDDEVIGFYSLIETHEEVTLEYFYIDSVNIRKGYGSHLWEHLV
                     EFCRSRGILDLQFVSSPEARHFYEKMGAVVVGEAKSRVLADRRILKWKFVI"
BASE COUNT          972 a          998 c          647 g         1174 t
ORIGIN      
        1 caaatttacc acttaaaaga gctccccatg caaccatgac acttataacc ctggagaggg
       61 cagtacccat tgccgcacct gcaaccccat atgcaggaaa tccaaacata ccgaatatga
      121 aaatatagtt tcctattaca tttatgatgt tcatcattat atcaatataa agtggggttt
      181 ttgtgtctcc tgctccctga aatattgacc ttgccatgaa atgaagcaaa aagaaaggga
      241 ctgccaggaa gaaaacctcc atatactcaa tggcataatt taagaccccg tctgtaacac
      301 ccagtatctt aaggctaaac tcgtttgtca acaagccaac aggtacgata aaagccgaca
      361 agaccaagga cagcataaaa gcctggcctg cagcaacact tgcaccctca tggtcatcct
      421 tgcctacaaa tctcgcaacc attgcggtgg ttcctgtgga tattgctatt accaatacca
      481 taaggatcat gattatattc ctgcttgttc caactgcaga tattgcatcc acaccaacat
      541 tcccgaccat cttcatgtct attgttccaa ccaaaacctg caagagcagc tggatgatta
      601 ccggccatgc cagataaagg gatgtcttgt acaggttgtc attgctcagt aggtattctc
      661 ttttcttttc atatgctgca cttgccatag tatctcctcc aaagtttcat tatatcattg
      721 gtaggaaaaa ttatctagac ccacgaaata tatttatggt tgaaagcact ttttaaagta
      781 cctatcttca taaagagcac cagctggatt atccagctgg tgctctttat gaaatatttt
      841 taattaaaga ctgaatttaa ctatcaaggc tggatcaaga actctgaatg caaaggtttc
      901 agctacaaag aatttaacct tttctgcatc gtgctcctga tagccgattg aaaagtctct
      961 tccgattgtc aattcaaggt cgtcatggtc gtaaggaatc aggtatgctc cctcaacaac
     1021 atggctcagt atgattttac cttcaatcag actttcaatt ctcttcttta gtggataacc
     1081 gctgtcctgg gcaattatcc tcttataggc atccttacct acaacaagtg tatacgatcc
     1141 gcctgcaaag tttcttctta gtctcaaaac tccctctgta attgattcca tcagggactt
     1201 tgcatcgtca cctagcttta tagccttacc ctctgctgct ccatcaagtc caactattcc
     1261 agcattctca aggccattgt acacagcatt ttcctcaaac agggctattt tctcaagggc
     1321 atcctccagt ggaccataat cgacatcctt cgctcctctt gatacgttgt caagctccca
     1381 tctgtccatc tcaaactcta ttcttgattc agtaagtggc agcaccttgt agtttgcata
     1441 gcaaacgtcc ccgtcctctt cagtatcacc aagtctgccc tctgatatta cgttgaatga
     1501 aagacccttt ggtccgttaa ccttaactac cttcctggct gacagatagg actttagaac
     1561 ctcagaggcc ctttcatcta tctcacccca ggcttcattt gatattggtg caagttctct
     1621 gtatagcatt tacgcttccc ccctatttta aatctccaat gtttaatccc ttatcaccgg
     1681 aaggttcttc tccttctccg ccctcttcaa ggtgggagat gtctccctca gtaaataagt
     1741 acgttttcat ttcctcatcc caggctccca tgtttcttct cagccactcc agggtcatta
     1801 ctgcgtgctc gatctcttca tccctattgt gggcaagtat ctccttaagg gcagggtcag
     1861 tggctgcagc tactctctgg ttataccagt caacagcctc aatttcctcc ttaaggctta
     1921 ccaacgctct agttatgtcc atagtcttgt catctagtct ttccaccggt tcatgatatt
     1981 gtgtcatccc tattcctcct tgtatttatt ctctaataaa gactttaccc tgaatttcca
     2041 aattataaac atcttacagc ctcctggtcc attccgcctt gaaatctccg tctgcaaggg
     2101 ctgtttccaa ggtttttatc atttcctcac ggtccctgtt caatgtcccc ttaagggcta
     2161 cgtagtttga agcgtggttg cttctaaata cacatcctgg agaatccagt tgacttagca
     2221 taagcagggt ctcctgggca atttcatagg gggagagcaa agaaaattct ccagactcca
     2281 cctggtccca aagttcagta ccaggctcag tcatcagtgt aagtagtcca aggtaatcag
     2341 gcctgatcat gttcactgcc cgggcggttt ccctggcgtg gtcttcccag ttttccctgc
     2401 ctccaagtcc cgaaataact gtaactgaga gtttcaatcc agcttccttt atcttccttc
     2461 ctgcggagat gatgccacct ctggtatcac ccttttttac agcctttagt atgtcatcgc
     2521 ttccagactc aagacccagg taggcaattc caagccctgc ttccttaagg gtcttaaggt
     2581 cagcatctga ttttaggtgg atgctttttg gggaagcata gattccaatc ctttcacatt
     2641 ctgggaaagt attttttagc tctccaatta tcaggacaag gtcttcagtc cggatcataa
     2701 gagcgtcccc atcggcaatg aatattcgct taaggttccc gtagagcctc cttgcttcat
     2761 gaatctcctg gattatctct tcggtttttc ttatcctgaa gctcttgtcc ttgtacatgc
     2821 tgcagaaggt acaccggttg tgggaacacc cgatggtggc ctgcaatatc cagctcctcg
     2881 cttctgatgg cggtctatat actgttcctt catatttcat ccaatcactc ccttgaacac
     2941 cataaaagta ggttacaata cactaaacag tataccataa cctacaccat ccctatttca
     3001 tattttcttt aatgtaatcc aagacttcct cagcatggcc cgctgacttc acctttctgt
     3061 attccttaac catctggcca tccctattga aaatgaaggt ggacctttca gttcccatgt
     3121 actccttacc atacattttc ttgggtacaa tcaccttgaa ctgctcatgc acctttcctt
     3181 cctcatcact taggaggaga aagttcaact cttgcttgtc cctgaacttc ttgtgggacg
     3241 aaaggctgtc cttgctaatg cccattatga cagtttccag ctcttcaaac tcctggttca
     3301 ggtccctgaa cccaacagcc tcggttattc agccggatgt attgtccttt ggatagaagt
     3361 agaccacaac attcctacct ttgaaatctt taagagatat ctccttacca tctgtttcct
     3421 ttaaagtgaa atcatattct gacatctgtt tacctcccct aatgttagtg aggattatat
     3481 acccaattgt tcaccattta caatcaaatg acaaacttcc acttaagaat tctcctgtct
     3541 gcaagaactc ttgattttgc ctctccaacc accaccgctc ccattttctc ataaaaatgt
     3601 cttgcctctg ggcttgaaac aaattgaagg tccagaatac ctctgcttcg acagaactca
     3661 acaagatgtt cccaaagatg gcttccgtag ccttttctaa tattcactga atcgatgtag
     3721 aaatactcaa gtgtaacttc ctcatgggtt tcaatgaggc tatagaaacc gatcacttca
     3781 tcatcattct c
//
LOCUS       DASRQF010000076         3363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_80104, whole genome shotgun sequence.
ACCESSION   DASRQF010000076 DASRQF010000000
VERSION     DASRQF010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80104"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VFD79_01950"
     CDS             <1..608
                     /locus_tag="VFD79_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92840.1"
                     /translation="SNHVHLLVKADMESLIRFFRRVNVKYAGNYNLFNGRVGHVFQDR
                     FKSEPVEDDTYLVQVVRYIHNNPVKAGIVSSPGEYQWSSYIEYTGPGKYLVNRAQKDF
                     VMSNISKDRLGFEKFHNVDDLNVYLDIKEDQKEIMQRNAQLIIEKHFRKSGLDALDQP
                     MKKKELLGEMVKEIYEKTGLSIREIARLLDVGVTTVRVYLK"
     misc_binding    721..971
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1044..2183
                     /locus_tag="VFD79_01955"
     CDS             1044..2183
                     /locus_tag="VFD79_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009307323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HZH92841.1"
                     /translation="MKIVNSWNDFDPLKHVIVGRADNCCIPPSEPASKAKVPLDSPMR
                     GMTGPRPLNTVEKANEQLENLVKVLEGLDIKVDRPEALQWNQAVVTPHFMTGSMFGCM
                     PPRDVLLTIGSDIISAPMSFRSRFFEHYAYSKNLREYFDKDPDFKWIAAPRPELGDAS
                     YDMHYFDDKVTEEILLERTAKLHMVTTEHEILFDAADVLRLGKDLFVQHGLTTNRKGM
                     EWLRRMYPDMRIHAVNFPGDPYPIHIDATFVPLKPGLILNNPHRKLPKEQRKIFEAND
                     WEIVEAAMPAHEQPPELCYSSVWLSMNCLVIDHKTVLVEESEKAQQEQMDKLGMNVIP
                     VPFRDAYAFGGGLHCATADVYREGECQDYFPNQVEDPTLVTFEGK"
     gene            2246..2371
                     /locus_tag="VFD79_01960"
     CDS             2246..2371
                     /locus_tag="VFD79_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92842.1"
                     /translation="MTGSWAINEFFIGCIVIMIVPLAVYIYREMTAVKEGDTSDI"
     gene            2361..>3363
                     /locus_tag="VFD79_01965"
     CDS             2361..>3363
                     /locus_tag="VFD79_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92843.1"
                     /translation="MIFNWIVFILAAAGMIGVGVWSNKKIKSQANHGGEGFLLGGKQI
                     GAFVGAGTLMATGYSGWGFIGSPGTAYAFGSIEVLANFFFAPAIVFGTLFFANFMRKN
                     AEKSGGLTVPEYLASTHRGTDGQKRLVHFFAGLATFIFLSVYMIGQIRAVGLVASQWL
                     DIPEVYASILLITVVAIFTIMGGLLAVALTDTIMCVGMLIASVIVFGVILQDVSLAEM
                     IRTVGQMNSELVNPTTSAPYGNTRYSVYLVFVYALLFTTTLPYMSVRFLSLKEDIKIH
                     KMALYMAPMGIILSLIPFVGMYMFYKDPTLANPDSAMPVFLNTFLSPALGGIITLFIL
                     "
BASE COUNT         1003 a          681 c          837 g          842 t
ORIGIN      
        1 tgagcaatca tgtccatctt cttgtaaagg ctgatatgga aagcctaatt agatttttca
       61 ggagagtcaa tgtcaagtat gccggcaact ataatctatt caatggcagg gttggccatg
      121 tatttcagga caggttcaag agcgagcctg tggaggatga tacatatctt gtacaggttg
      181 taagatatat acacaacaat cctgtgaagg ctggaatcgt atcaagtcct ggtgaatacc
      241 aatggagcag ctacattgaa tacacagggc caggaaaata cctggtcaat agggctcaga
      301 aagactttgt gatgtccaat atctcaaagg ataggctagg ttttgagaaa tttcacaacg
      361 tggatgatct gaatgtttat cttgacatta aggaggatca aaaagagatc atgcaaagga
      421 atgcacagtt gatcatagaa aagcatttca ggaaaagtgg tttggatgct ctggatcaac
      481 ccatgaaaaa aaaggagctg cttggagaaa tggtgaagga aatatatgaa aagactggct
      541 taagcataag ggaaatagca aggctactgg atgttggggt caccactgtt agagtatacc
      601 taaaataaca caaaaaacac aaaaagaacc ggtccctttg tgttttttga aagaatgaaa
      661 aataaccttt acaagaccaa tggcatggtg tataatcaca actaataatt aaatatcaaa
      721 caaaaagctt tgatgagaac agtagcttca ggaaagtctt tcagcgagtc ccggttggtg
      781 gaaggggata ggatattaat gaagtgaaga gggcctcaga gcattgcacc gattcatttg
      841 taggctgcaa cgggttcacc cgttatagtg atagagtata acacatttag tcatgtgcgt
      901 acttgatgag gcttataccg tgaggtgtag gcgaattagg atggtaacac ggcatataac
      961 ccgttccttg gaatatatat attctttgga acgggttttt taatacccaa aaataataat
     1021 acataatttt aggaggagaa aagatgaaga ttgtaaattc atggaacgat tttgacccac
     1081 ttaagcatgt gattgtaggt agagcggaca actgctgcat acctccctca gagccggcat
     1141 caaaggcaaa ggtacccctt gattcaccca tgaggggaat gacgggacca agacctttga
     1201 atacggtgga aaaggccaac gagcagcttg aaaaccttgt taaggtcctg gagggtctgg
     1261 acatcaaggt tgacagacct gaggccctgc aatggaacca ggctgttgtg accccccact
     1321 tcatgacagg aagcatgttc ggctgcatgc cgccaagaga tgtgcttctt acaataggaa
     1381 gcgacatcat atccgcacca atgtcattca gatccagatt cttcgagcat tatgcctact
     1441 caaagaacct gagagagtat ttcgacaagg atcctgactt taaatggata gcggcaccaa
     1501 gacctgagct aggagatgca agctatgaca tgcactattt tgacgacaag gtaactgagg
     1561 agatactcct tgagagaacc gccaagctcc atatggtgac taccgagcat gaaatactct
     1621 ttgatgcggc ggatgtcctg agacttggca aggacctttt cgttcagcat ggacttacca
     1681 caaacagaaa aggaatggaa tggctcagaa gaatgtatcc tgacatgaga atacatgctg
     1741 tgaacttccc tggagaccca taccccatcc atatagatgc tacatttgtt ccactgaagc
     1801 caggcttgat actcaacaat ccccacagaa agcttcccaa ggaacaaaga aagatatttg
     1861 aggcaaatga ctgggagatt gtcgaggctg caatgcctgc ccacgagcag ccacctgagc
     1921 tttgctactc aagcgtatgg ctgtcaatga actgtctggt catagaccat aagacagtcc
     1981 ttgtggagga aagtgagaag gcacaacagg aacagatgga caagcttgga atgaacgtaa
     2041 tccctgtacc atttagagat gcctatgcct tcggtggagg gctacattgt gcgacggcgg
     2101 atgtctacag agaaggtgaa tgccaggatt acttcccaaa ccaggtggaa gacccaactc
     2161 ttgtaacatt tgagggaaaa tagatgatga tggggtgggt gaaacaccca tcccacatga
     2221 aaacatagtg aaggaggaag agatcatgac aggatcatgg gcaataaacg agttttttat
     2281 tggttgcata gtgattatga tagtaccttt ggcggtgtac atctacagag agatgacagc
     2341 tgtcaaggaa ggtgatacaa gtgatattta actggatagt gtttatactg gcagctgcag
     2401 ggatgatagg tgttggggta tggtcaaaca aaaagataaa atcacaggca aaccatggag
     2461 gagaggggtt ccttcttgga ggcaagcaga taggagcatt tgtcggagcg ggaaccctaa
     2521 tggcaaccgg ttacagtgga tggggattca ttggctctcc aggaaccgcc tatgcctttg
     2581 ggtccattga ggtgcttgcc aatttcttct ttgcccctgc aattgtcttt ggaacccttt
     2641 tctttgccaa tttcatgcgg aaaaatgcgg aaaagagcgg tggcttgacc gtacccgagt
     2701 accttgcatc aacccacagg gggactgacg ggcagaagag gctggtccac ttcttcgcag
     2761 ggcttgcaac cttcatattc ctttcagttt acatgatcgg acagatccgg gcagtgggcc
     2821 tggtggcatc ccagtggctg gacataccgg aggtgtatgc atccatactc cttattacgg
     2881 tggtggcaat attcacaatc atgggtgggc ttcttgccgt agcacttact gataccatca
     2941 tgtgcgtagg aatgctgatt gcctcggtca tagtctttgg tgtgatcctt caggatgtat
     3001 cccttgccga aatgataagg acggtgggcc agatgaactc ggagcttgtg aatccaacca
     3061 cctcggcgcc ctatgggaat acacgttaca gcgtatacct ggtatttgtg tatgccctgc
     3121 tctttactac caccctgcca tatatgtcag tgagattcct ttccctcaag gaggatataa
     3181 agatccacaa gatggcactc tacatggcac caatgggaat catactaagc cttataccat
     3241 ttgtggggat gtatatgttc tacaaggacc caacccttgc aaatcctgac agtgcaatgc
     3301 cggtattttt gaacacattt ctttcacctg cattgggagg aatcataacc ttgttcattc
     3361 tct
//
LOCUS       DASRQF010000077         2657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1571353, whole genome shotgun sequence.
ACCESSION   DASRQF010000077 DASRQF010000000
VERSION     DASRQF010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1571353"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VFD79_01970"
     CDS             complement(<1..423)
                     /locus_tag="VFD79_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HZH92844.1"
                     /translation="MNELTVNYLCKQYGLDKDVVEFVNRMEELVKARFKTLEEIREYN
                     QYKVIGAMQDNRLSSTDFHWTTGYGYGDIGREKVESIFADVFKAEAALVRPAIASGTH
                     ALNLALSGILRPGDQLLYITGTPYDTLQKVIGVKGDVEG"
     gene            complement(514..735)
                     /gene="hfq"
                     /locus_tag="VFD79_01975"
     CDS             complement(514..735)
                     /gene="hfq"
                     /locus_tag="VFD79_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HZH92845.1"
                     /translation="MKQGVNLQDQFLNSARREGVLITVFLVNGYQIKGYVKGFDSYTI
                     VLDADEKQQLVYKHAISTIIPNSVISFDH"
     gene            complement(750..1688)
                     /gene="miaA"
                     /locus_tag="VFD79_01980"
     CDS             complement(750..1688)
                     /gene="miaA"
                     /locus_tag="VFD79_01980"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HZH92846.1"
                     /translation="MKEKLIVITGPTAVGKTSISISLAKRLNGEIISADSMQIYKGMD
                     IGTAKVTEAEMDGVPHHLIDFVYPDQQFTVADYKENATKIIQSLNKEGKLPIIAGGTG
                     LYINSLVYELNFVDVPPDQDLRDKLENLAYEEGNRALLEKLEKIDPLAASKYTENDIQ
                     RIVRAIEITTLSGKTYHEYGSNFRTQNDSYDLVMYCLNLDRQKLYERINQRVDIMLQL
                     GLVNEVRGLMDKGYGKDLVSMKAIGYKEIMEYLEGNESYDDVVEKIKKLSRNYAKRQL
                     TWFRRDNRINWVEVEEYGRKDAIVDYLVSDIRSRIY"
     gene            complement(1698..>2657)
                     /locus_tag="VFD79_01985"
     CDS             complement(1698..>2657)
                     /locus_tag="VFD79_01985"
                     /inference="COORDINATES: protein motif:HMM:NF020260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HZH92847.1"
                     /translation="DPSDVDVNIHPTKQEIKFTNDSNIYQKLEAAIRNNIDVIMRVPK
                     ANLEPKVKNNLEEYPSIWDIEKDGTLDKLENNRYNDDPDEKSIVFVDYSQEEDWSPND
                     LTKEKKESYSSHGIEGSIDKTMVSFKSLRILGTAFRTYVFAENVVNSTLYIIDQHAAH
                     ERIMYEKLLNEFKNETVHVQQLLQPLVLDLGQTEYTIAMENLDHFESLGFLVEPFGDR
                     SMILRGVPFVFGAPDSRSLFLDILDQLEESKPSSYETKLERIMKLACTSAIKAGDSVA
                     EIEAKALLNQLEECENPYTCPHGRPTIIEMTRKELEKMFLRIT"
BASE COUNT          666 a          636 c          458 g          897 t
ORIGIN      
        1 cccctccaca tcacccttga caccaatcac cttctgcaaa gtgtcatatg gggttcctgt
       61 aatatataag agctgatcac caggcctaag tatccctgaa agggccaggt taagggcgtg
      121 ggtcccagaa gctattgcag gcctaaccaa ggcagcctcc gccttgaata catctgcaaa
      181 gattgactct accttctccc ttcctatgtc tccatatcca tagccggtgg tccaatggaa
      241 gtcagttgat gagagtctgt tgtcctgcat tgcaccgatt accttgtatt ggttatactc
      301 cctgatttct tcgagtgttt tgaatctagc cttaacaagt tcctccatcc tgttcacaaa
      361 ttctacaaca tccttatcta agccatattg tttacataaa taatttactg tcaactcgtt
      421 catgttttct ccttagaatt taaatttgac aaaagaacag tctctgtcac cagagactgg
      481 gttgatgctt tttatcgcat ggtggatgtg attctagtgg tcaaagctaa ttacactgtt
      541 tggaattatt gttgaaatag catgcttata aaccaactgt tgcttttcat cagcatcaag
      601 aactatggta tagctgtcaa aacctttgac atagcccttg atttgatatc cattcacaag
      661 aaaaactgtt attagtaccc cttctctcct ggcagaattt aggaactggt cctgtaaatt
      721 aactccctgc ttcatttgaa aatccccctt taatagatcc gactcctgat atcactaacc
      781 aaataatcaa caatagcatc cttcctaccg tattcttcaa cttctaccca atttattcta
      841 ttatcccgcc taaaccatgt cagctgtctt tttgcgtaat tcctggaaag ctttttgatc
      901 ttttccacaa catcgtcata actttcattg ccttccaggt actccataat ctctttatac
      961 ccaattgcct tcatggacac caaatctttg ccataaccct tgtccataag gccacgaacc
     1021 tcattgacca aacccagttg gagcatgata tcaacccttt ggtttatccg ttcatatagc
     1081 ttttgcctat ccaagttcag gcaatacatc accaggtcat aactgtcgtt ttgtgtcctg
     1141 aaattacttc catactcatg gtaggtttta cctgaaagtg tggttatctc tatggccctt
     1201 acaatccttt ggatgtcgtt ttcagtgtat ttacttgcag caagaggatc aatcttctcc
     1261 agtttttcaa ggagtgccct attcccttct tcatatgcca agttttccaa tttgtctctt
     1321 aaatcctggt ctggtggcac atcaacaaag ttcagttcat atacaagtga gtttatatat
     1381 aggccggtac cgcctgctat gataggcaat ttaccttctt tattaagaga ctgtattatc
     1441 tttgttgcgt tttctttata atcagccaca gtgaactgct gatccgggta aacaaagtca
     1501 atcaaatggt gtgggacacc atccatctca gcttctgtta ccttggcagt accaatatcc
     1561 attcccttgt atatctgcat ggaatcagct gatatgatct caccattcaa tctctttgcc
     1621 agtgatattg atatggatgt ttttcctact gctgttggtc ctgtaattac aattagtttt
     1681 tccttcataa ttctcaccta tgttatcctg aggaacattt tttcaagttc cttcctggtc
     1741 atttcgatta ttgtcggtct accgtgagga catgtatatg gattctcaca ttcctcaagc
     1801 tggtttagaa gtgcctttgc ctcaatttcc gccacactgt cccctgcctt tattgcactg
     1861 gtacaagcca gtttcatgat tctttccagt tttgtctcat agcttgaggg tttactttcc
     1921 tcaagctggt ccaatatatc aagaaacagg ctcctgctgt ctggagctcc gaaaacaaat
     1981 ggtacacccc taagtatcat gctcctgtcc ccaaagggct ccaccaggaa tccaaggctt
     2041 tcaaaatggt caagattctc catggcaatg gtgtattccg tttggccaag gtccaataca
     2101 agtggctgaa gaagctgctg aacatgtact gtctcattct tgaattcatt taggagcttt
     2161 tcatacatta tcctttcatg agcggcgtgc tggtcaatta tatagagggt tgagttcaca
     2221 acattttctg caaagacata ggtcctgaat gccgtcccca gtatccttaa agatttgaag
     2281 ctaaccatgg tcttatcaat tgaaccctca atcccatgtg atgagtagga ttcctttttc
     2341 tctttggtta gatcatttgg ggaccaatcc tcttcctgtg aataatcaac aaatacaata
     2401 cttttctcat cgggatcatc attgtatcta ttgttctcaa gcttatccaa ggtcccatcc
     2461 ttctcaatat cccatatgga ggggtattcc tccaggttgt ttttcacctt aggctccagg
     2521 ttagcctttg gaacacgcat tatgacatca atgttgttcc tgattgctgc ctcaagcttc
     2581 tgatatatgt tactgtcgtt tgtaaatttg atttcctgtt ttgtcggatg gatattcaca
     2641 tctacatccg atgggtc
//
LOCUS       DASRQF010000078         2565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_322952, whole genome shotgun sequence.
ACCESSION   DASRQF010000078 DASRQF010000000
VERSION     DASRQF010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322952"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..464
                     /locus_tag="VFD79_01990"
     CDS             27..464
                     /locus_tag="VFD79_01990"
                     /inference="COORDINATES: protein motif:HMM:NF024312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92848.1"
                     /translation="MAKTKLVYGAAEGRSMISQIQGKVKFGYIPRVLNKATHESSYMM
                     NQKIFESVIELIKDKNTIEYDEDLEVYTAYNTIVPQIYGEGATKEEAIDQMLEGAKEF
                     AKDYDENIEVFSGVLNGIQQLIISYILLNLDDENKIREILKVA"
     gene            457..690
                     /locus_tag="VFD79_01995"
     CDS             457..690
                     /locus_tag="VFD79_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92849.1"
                     /translation="MPDSVTQGQFKRFLKRNQFVSKGKKNNLYFGMLDGKPRAVVFHY
                     HKDNDIIPTGTLSAIARSLELSKTELIDRIKAR"
     gene            721..1311
                     /locus_tag="VFD79_02000"
     CDS             721..1311
                     /locus_tag="VFD79_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HZH92850.1"
                     /translation="MKKIKVFSDFACPFCYIGFSIAEKLVKENSEVEFHWYPYELDSD
                     APEEGTSLEDAIPKEQIDMAYRRIERLGSEYGLVYTNKTTKFNTGLLHRAALYAQEVG
                     SFYPFAKEAFKAIFEEGKNVALKEVVNQIGFDAGLNIHEMIEAIEAGEFEEMMEESRV
                     LAVAHEIESVPTFIREDGNKITLLKGYDKFRKDLLD"
     gene            1441..>2565
                     /locus_tag="VFD79_02005"
     CDS             1441..>2565
                     /locus_tag="VFD79_02005"
                     /inference="COORDINATES: protein motif:HMM:NF019450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HZH92851.1"
                     /translation="MRKILSMLIGLLMVLFIGYTIYTEFNEVQDNDIIESDEFFVLLE
                     DMKLIEAEDYIYIDDQIHFSVHFLKEFVDQNLFYDENEDVLVFTDDSRVGRFYPDSQS
                     GTMNGKEILLKSPIKIVDKDTFIPYDLIRELYSERLEFYWNNSSLVIDFKKYDYLEGE
                     ILEDSYLYSSPHKRAPLIGEGLVEGDRVIVFSEDRSWYKVRTNEGLVGYVMGESILIE
                     YGKNRFKRSVDREEGTNSGDPGMINLTWDYTYGPMRNMDAAPDMKGVNVVSPTWFDVS
                     DENGSIYDKGNLDNSILYKNAGYELWPAVTNSFDPGMTSVFLRSSESRERIINDLLDL
                     YEYYKVDGINIDFENIYYEDSDHLTQFVRELYPVFKDQGMK"
BASE COUNT          833 a          375 c          615 g          742 t
ORIGIN      
        1 attaatatat gaaaggtgtt ggtgttatgg ctaaaacaaa attagtctat ggtgctgctg
       61 aaggtcgctc aatgatctca cagatccaag gcaaagttaa gtttggttat ataccgaggg
      121 tcttaaacaa ggccactcac gaatccagct atatgatgaa ccagaagatt tttgaaagtg
      181 ttattgagct tatcaaggac aaaaatacaa tcgaatatga tgaagattta gaggtttata
      241 cagcctataa tacgatagtt ccgcagatat acggggaagg agctactaaa gaagaagcta
      301 tagaccagat gctggaggga gccaaggagt ttgccaagga ctacgatgag aatattgaag
      361 tgttttcagg tgtccttaat ggaattcagc aattaataat tagctatatc cttctgaatc
      421 tggatgatga aaacaagata agggagatac taaaggttgc ctgattcagt aacccaggga
      481 cagtttaaaa gatttctgaa gagaaatcaa tttgtctcaa aggggaagaa gaacaatctt
      541 tattttggta tgctagatgg caaaccccgt gcagtcgttt ttcattatca taaggataat
      601 gatattatac caactggtac tttgtctgca atagccaggt cattagagtt atccaagact
      661 gaattgattg atagaatcaa ggcaagatag agctggcaag gtaggttaag gagtgataat
      721 ttgaaaaaga tcaaggtttt ttcagacttt gcatgtccat tttgctatat aggtttttca
      781 atagctgaaa aactggttaa ggagaattca gaagtggagt tccactggta tccttatgag
      841 ttggattctg atgctcctga ggaaggtaca tctctggagg atgcgattcc aaaggaacag
      901 atagatatgg catacagaag aattgagagg ttgggttcgg agtatggtct tgtctatacc
      961 aataaaacta ctaaattcaa taccggtctc ttgcacaggg ctgccttgta cgcacaggag
     1021 gtaggaagct tttacccatt tgccaaggaa gctttcaagg caatatttga ggagggaaag
     1081 aatgttgctt tgaaggaggt tgttaatcaa ataggatttg atgctggatt gaacattcat
     1141 gaaatgatag aggcaattga agcaggagag tttgaagaaa tgatggaaga atccagggtc
     1201 cttgctgtag cccatgagat agagagtgtc cctaccttca taagggagga cgggaataaa
     1261 ataactcttc ttaagggata tgataaattc aggaaggacc tcctggacta gcatattatg
     1321 tgacgcccat taaggtatat agatggacct taatgggcgt ttctattttt caaaggctat
     1381 ttgaaattcg aagctaactc taaagcgtat catggtataa taaaaccagg tggtgaaaaa
     1441 atgaggaaaa tactatccat gttaattggt ttgcttatgg ttttgtttat aggatataca
     1501 atttatacgg aatttaatga agtccaggat aatgatatta ttgaaagtga tgagtttttt
     1561 gttctccttg aagacatgaa gcttattgaa gctgaagatt acatctatat tgatgatcag
     1621 atccactttt cagtacattt tttaaaggag tttgtggatc agaatctctt ttatgatgaa
     1681 aatgaggatg tcctggtttt tactgacgat agtagagtgg gaagattcta tccggatagt
     1741 caaagtggaa caatgaatgg caaggaaatc ttgctaaaat cacccataaa gattgttgat
     1801 aaggatacat ttattccata cgacctgatc agggaattat acagtgaaag gttggagttt
     1861 tattggaaca atagctcctt ggttattgac ttcaagaagt atgattacct ggaaggagaa
     1921 atcttggaag attcatatct ttattccagt ccacacaaga gagcaccact aattggggaa
     1981 ggtcttgtag agggtgacag ggttattgtt ttttcagagg acaggtcttg gtataaggtc
     2041 agaacaaatg agggtctggt tggatacgtt atgggagagt caatacttat tgaatacggt
     2101 aagaacagat tcaaaagaag tgttgacagg gaagagggga ccaattctgg tgatcctggt
     2161 atgataaatc ttacatggga ctatacatac ggtccaatga gaaacatgga tgcagctcca
     2221 gacatgaaag gggttaatgt agtttcacca acatggtttg atgtttctga tgagaatggc
     2281 agtatctatg ataaggggaa tttagataac tctattctct acaagaatgc tggctatgag
     2341 ctatggccag ctgtcaccaa ttcctttgat cctggaatga ccagtgtctt tcttaggtcc
     2401 agcgaatcaa gggaaaggat aataaatgac ctgttggact tgtacgagta ttacaaggtt
     2461 gacggaatca acatagattt tgagaacata tactacgagg acagtgacca ccttactcaa
     2521 tttgtgagag aactctaccc ggtcttcaag gaccagggga tgaag
//
LOCUS       DASRQF010000079         3653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1318983, whole genome shotgun sequence.
ACCESSION   DASRQF010000079 DASRQF010000000
VERSION     DASRQF010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318983"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VFD79_02010"
     CDS             <1..695
                     /locus_tag="VFD79_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92852.1"
                     /translation="KEYAQISADAKIEALVNPMASRAWDGANQYKTPEGYVSGMRLRG
                     QDNMYLWLKEFDRCLNAGIRGFLITDESVLHMVVKMRAAGLIPAETKFKVSVFAGHGN
                     AVAAKMLEDMGADSFNPLADLTLPMFASIRSTTNIPMDVYMSLVDSMGNIQRHVDSDE
                     IARICAPVYFKFEPGKNEMDIYNAWHDDEPLDKLIPVKIRMAKIAKEWCDESDTELVF
                     NDYKEDLAIPRP"
     gene            711..1946
                     /locus_tag="VFD79_02015"
     CDS             711..1946
                     /locus_tag="VFD79_02015"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HZH92853.1"
                     /translation="MSNNPFETALTTLKEASELANLDPNVVKYLSKPKRIFQFTIPMR
                     MDNGDYEIFDAYRVHYNDALGQVKNGMRFVPDLDLDIVKALGFWMTIKHAVAGIPAGG
                     GKGGVKVDARKLSEGELERISRAFIRKLPVKGAWVDVPGADIGTSAKTMAWMLDEYEE
                     INGFHSPAAINDKPAETGGTLGSMEATGTGAYFVTMEVMKDNNIPKGATIAMQGFGNV
                     GRIAARLLHDEGYKVVGVADLTGGVYDSNGLDIPKLEAYVAETKQIEGFPCEKTLDLN
                     SVLELDVDILMPAAVQSVVHEGNADNVKAKVIMECANGPVTPEAEKILEKKGTLIVPD
                     VLTNCGSAIVCSFERTQGLTDDYWELDKVNEKLKEQITKAYKNTVETAASENTSMRNA
                     AWINALRKLEKSMKARGWV"
     gene            2062..2883
                     /locus_tag="VFD79_02020"
     CDS             2062..2883
                     /locus_tag="VFD79_02020"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZH92854.1"
                     /translation="MATSKSQHTPKYPVLTIDKALELMEILAEEGYQEGMGVSELSTR
                     LDIGKSTVHRILDTLAARNYLEQDHETKKYRLGWRLFEIGNVIPRQRNLYNFDMGILQ
                     KLCNQYQETVNLGVRVEDSVVTVSKVSAKSSLIANLQVGSRESLHATAMGKALMSDMT
                     REEVIDILGEGPYTKYTSFTFTEIDTLMEELRKIRKQGFSIDDEEFAPGLTCIAMPVR
                     NFDNEIVAAVSVSGASIRQTYSKIDDIKVGLKEATEKLSYFLGWEGKIPETSDTK"
     gene            2913..>3653
                     /locus_tag="VFD79_02025"
     CDS             2913..>3653
                     /locus_tag="VFD79_02025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HZH92855.1"
                     /translation="MDWIKEQSEELQEELVELRRDFHAYPELGFEEHQTSRKVEEYLQ
                     NLGLKTERVAGTGVVAMIEGHEDGPVLMLRADMDALPIQEENTVAYASTNPGVMHACG
                     HDAHMAMLMGAAKILSENKELIKGKIKLVFQPNEENAGALPMIEEGVLDNPKVDAAVG
                     VHIWTPLPSGVVGISAGGVMSGLYIFRIRVKGYGGHSGYPETAIDPIIAASDIVLSSQ
                     RILTREISAMRPITLTFGQINGGTKANII"
BASE COUNT         1208 a          675 c          950 g          820 t
ORIGIN      
        1 tgaaagaata tgcacagata tccgcagatg caaagataga agcacttgta aacccaatgg
       61 cttcaagggc atgggatgga gccaatcaat acaagactcc agaaggatat gtttcaggaa
      121 tgaggcttag aggccaggac aacatgtacc tgtggctgaa ggaatttgac cgttgcctga
      181 atgcagggat cagaggattc ttgatcactg atgaaagtgt actccatatg gttgtaaaaa
      241 tgagagctgc aggcctaata cctgctgaaa ccaagttcaa ggtttctgta tttgccggcc
      301 acggaaatgc agtagctgcc aagatgcttg aagatatggg agcagacagc ttcaacccat
      361 tggctgacct tacacttcca atgtttgcct caataagaag cacaacaaat ataccaatgg
      421 acgtatacat gagtcttgtt gattcaatgg gtaacattca aagacatgtt gattcagatg
      481 agatagcaag aatatgtgct ccagtctact tcaagtttga gcctggaaag aacgagatgg
      541 acatctacaa tgcttggcat gatgatgagc cattggacaa gctgatacca gttaagatac
      601 gtatggcaaa gatcgccaag gaatggtgtg atgagagcga tactgaactg gtattcaacg
      661 actataaaga agaccttgca attccaagac cataaaggtg ggtgttaata atgagtaata
      721 atccttttga aacggctttg accaccttga aggaggcaag tgaattagca aatcttgacc
      781 ccaatgtggt gaagtaccta tcaaaaccaa aaagaatctt ccaattcacc attccaatga
      841 gaatggacaa tggtgactat gaaatatttg acgcatacag ggtccattac aatgatgcgc
      901 tgggtcaggt gaagaacggt atgagatttg tacccgacct tgatcttgac atagtaaagg
      961 cactgggatt ttggatgaca atcaagcacg ctgttgcagg gatacctgca ggaggcggca
     1021 agggcggagt aaaggtagat gctcgcaagc tttccgaagg tgagctagaa agaatatcca
     1081 gagccttcat aagaaagctt cctgtaaagg gagcatgggt cgatgttcca ggtgcagaca
     1141 ttggaacaag tgccaagaca atggcctgga tgcttgacga gtatgaggag atcaatggtt
     1201 tccacagccc tgcagcaatc aacgacaagc ctgcagaaac aggtggaacc ctgggaagca
     1261 tggaagcgac aggaacaggt gcctactttg tgaccatgga ggttatgaag gacaacaata
     1321 ttcctaaagg cgcgacaata gcaatgcagg gatttggaaa tgttggaagg attgcagcaa
     1381 gactcttgca tgacgaaggc tacaaggttg ttggagtagc cgaccttaca ggcggggttt
     1441 atgactccaa tggactggac ataccaaagc ttgaggcgta tgtcgctgaa accaagcaga
     1501 ttgagggatt cccatgtgag aagaccctag atctgaacag cgtactggag ctggatgttg
     1561 acatacttat gccggcagcg gttcaaagtg ttgtacatga gggaaatgca gacaatgtta
     1621 aagccaaggt aatcatggaa tgcgcaaacg gtcctgtaac accggaagcg gaaaaaattc
     1681 tggagaagaa gggtacgctg atagttccag atgttcttac aaattgcggg agcgcaatag
     1741 tttgctcctt tgagagaacc caaggtctta cagatgacta ctgggagctt gacaaggtaa
     1801 atgaaaagct taaggaacag attacaaaag cctataagaa cacggttgaa acagcggcaa
     1861 gtgaaaatac atcaatgcgt aacgcagcat ggataaatgc acttagaaaa cttgaaaaat
     1921 caatgaaagc ccgaggctgg gtttaaaaaa taagggaagg aggctttctc catggaaagc
     1981 ctttctccaa ccctggttct ttggtacaat agacctatat taatagaaaa tgtacaaaga
     2041 atattggaaa gggtgactat tatggctaca agcaaatccc agcatacacc aaagtatccc
     2101 gtgttgacca tagataaggc tttggagcta atggagatat tggccgagga aggctaccag
     2161 gaaggaatgg gcgtaagcga actcagcaca agactggata ttggaaaaag cacagtacac
     2221 cggatattgg atacacttgc agcgagaaac tatttggaac aggatcacga aaccaagaaa
     2281 tatcgtttgg gatggcgatt atttgaaatt gggaatgtga tacctcgaca gagaaaccta
     2341 tataattttg acatgggtat cctgcagaag ttatgtaacc agtatcagga aacagtaaat
     2401 cttggagtac gtgttgagga cagtgtcgta accgtttcaa aggtaagcgc caagtcaagt
     2461 cttattgcaa acctgcaggt gggttcaaga gaatcactgc atgccacagc tatgggtaag
     2521 gccttgatgt cggatatgac aagggaagaa gtaattgata ttcttgggga aggaccatac
     2581 acaaaataca ccagcttcac cttcacagaa attgatactc tcatggagga gctaaggaag
     2641 atccggaagc agggattcag catagatgat gaagagtttg caccaggact tacatgcata
     2701 gcgatgccgg ttagaaattt cgacaatgag attgtggcgg ctgtcagcgt aagtggtgca
     2761 agtatccgac agacatacag caaaattgac gacataaagg taggactaaa ggaagcgaca
     2821 gaaaaactgt catatttcct tggctgggaa gggaaaattc ctgaaacctc ggatactaaa
     2881 taaagtcaca ttaagggagt tggctttaag aaatggactg gatcaaggaa cagagtgagg
     2941 aactgcaaga agaactggtt gagcttagaa gagatttcca tgcatacccg gaacttggat
     3001 ttgaggaaca tcaaaccagt agaaaggttg aggaatacct tcagaacctg ggtttgaaaa
     3061 ctgaaagggt tgcagggacc ggagtggttg ccatgataga aggccacgaa gatggacctg
     3121 tgctaatgct aagggctgac atggatgccc tgccaattca ggaggaaaac acagtagcat
     3181 atgcttcgac caatcctgga gtcatgcatg catgcggaca tgacgctcac atggctatgc
     3241 tcatgggagc ggcaaaaata ttatcggaaa acaaagagct gataaaggga aaaataaaat
     3301 tggtttttca acccaatgaa gaaaatgcag gagctttacc catgattgag gaaggagttc
     3361 tggacaatcc caaggtggat gcagcagtgg gagtacatat atggacccca ttgccatcgg
     3421 gagttgtcgg gatatcagct ggaggagtaa tgtccggctt gtacattttc aggataaggg
     3481 taaagggtta cggaggccac tcaggctatc ctgaaactgc aatagaccca ataatagcag
     3541 catcagatat tgtattgtcc tcccaaagga tattgacaag agagataagc gctatgcgac
     3601 caatcacctt gacctttggc cagataaatg gtggaaccaa ggcaaatata ata
//
LOCUS       DASRQF010000080         3463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_341732, whole genome shotgun sequence.
ACCESSION   DASRQF010000080 DASRQF010000000
VERSION     DASRQF010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341732"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(328..1323)
                     /gene="trpS"
                     /locus_tag="VFD79_02030"
     CDS             complement(328..1323)
                     /gene="trpS"
                     /locus_tag="VFD79_02030"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004826341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HZH92856.1"
                     /translation="MEKKIVFSGVQPSGALTIGNYIGALRNFTELQDEYDCNYCVVDM
                     HAITVPQVPKNLRKNTLDVLAMYLASGIDPDKSTLFIQSHVPAHAELSWVLNTVTYMG
                     QLNRMTQFKEKSRKSDENLNAGLFTYPVLMAADILLYQADFVPVGEDQKQHLELARDV
                     AERFNNKYSDTFTVPEPLIKEFGARIMSLQDPTAKMSKSDSDANAYVLLQDGKDQIAK
                     KIKRAVTDSVGSVNYTDDQPGIKNLINIHSVLSGKSVDEIVQLYSGKGYGVFKSELAE
                     VVIDALDPIQTKYKEYLDNRDFLEKVYSEGANKAEAKARKTLRKVYKKIGFIPRV"
     misc_binding    complement(1369..1567)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1667..2824)
                     /locus_tag="VFD79_02035"
     CDS             complement(1667..2824)
                     /locus_tag="VFD79_02035"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HZH92857.1"
                     /translation="MIDKFDFTLPTTIRYGAGSLNLLGEELKKLEANRIMIITDKGIV
                     RSGLLDRLEGILKSEGYEYLVFDEIEANPKDYNVEECAKTIRDNKVDTLVAFGGGSPM
                     DAAKGAAVVSRQGGRIRDYQGKDKIGKDCMPLITIPTTSGTGSEVTFSSVITDTERNF
                     KFTVKSPHIAAKVAILDPELTISVPPLITAATGIDALTHAIEGYTANCTEPLAEAFGL
                     YAVEYIASSISRAVSDGEDLEARDKMMMGSLMGGLSFSHSDVASVHCMAEALGSLYDA
                     PHGLCNSILLPYVMEYNLPECEHKYARIARAMGIRDNDDKIAAEKGIEYIRNLSEEIG
                     LPGLSSLDIDPKDFERLAEMSVENGSNASNPRKMDKSTYTQLFRIAYEDRR"
     gene            complement(2842..3084)
                     /locus_tag="VFD79_02040"
     CDS             complement(2842..3084)
                     /locus_tag="VFD79_02040"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HZH92858.1"
                     /translation="MSDNLDLVVLRTIESEFEMNMITAILEDNGIPFIVKNKGSGSYM
                     RIITGSSPFKTDILVSRDSFERSMELIIPIIGDDYE"
     gene            complement(3129..3266)
                     /locus_tag="VFD79_02045"
     CDS             complement(3129..3266)
                     /locus_tag="VFD79_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92859.1"
                     /translation="MTKANLINFITIGLTFVFSSTIITTKLAQGKIDELEVGRDKYRS
                     Q"
     gene            complement(3263..>3463)
                     /locus_tag="VFD79_02050"
     CDS             complement(3263..>3463)
                     /locus_tag="VFD79_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92860.1"
                     /translation="IFLFDLIPTIEKLDMENLKGILITEDERKALVSNTGFFEVEDNH
                     LIAEDEQFIYHIERISNTDSYL"
BASE COUNT          931 a          824 c          547 g         1161 t
ORIGIN      
        1 aaattacctc cagtaaattt ttagtaacta atttttaaca cttgcaaatc taatacaaga
       61 cttctccatt tccggaaatg gtacgtcttt ataacgttgc cattattata agcaagtagt
      121 gtgccaaagt tttccctatc ctgttaatta attatatttt tttgtcctca ctctgagatt
      181 actgaatttt caagcttcag cactatagat aatttatcca tatttgattt tatctagctg
      241 agtatactgt ttcaaaaaat aaaaaagcag aaccttttca tattgaaatg ctagattgaa
      301 gttctgcttt gttttttgaa attaaattta aactcttggt atgaatccga tttttttata
      361 gactttcctt aaggtttttc ttgcttttgc ctcagccttg ttggctccct cagagtaaac
      421 cttctcgagg aaatccctgt tatcaagata ttccttatac tttgtctgga ttggatcaag
      481 ggcatctatc actacctcag caagctcaga cttgaaaact ccgtaaccct ttccgctata
      541 tagctggact atttcatcaa cactctttcc tgacagcact gaatgtatgt ttatcaaatt
      601 ctttattcca ggctgatcat ccgtatagtt aacacttcca acagaatcgg tcactgcccg
      661 ttttatcttc ttggcaatct gatccttgcc atcctgtagt aatacatatg catttgcatc
      721 gctgtcagac ttgctcattt ttgccgttgg gtcctggagg ctcattattc ttgcaccgaa
      781 ttcctttatc aatggctcag gcacagtaaa tgtgtcacta tacttgttgt tgaatctctc
      841 tgctacatcc cttgcaagtt caagatgctg cttctggtct tctccaactg gtacgaagtc
      901 agcctgatac aatagaatat ccgcagccat cagtactggg taggtaaata atccagcatt
      961 aaggttttca tcagattttc tcgacttctc cttaaattga gtcatcctat tcaattgtcc
     1021 catatatgtt actgtattaa gcacccagga aagctcagca tgggctggaa cgtgggactg
     1081 aatgaacaat gttgatttat cgggatcgat tccacttgcc agatacatgg ccaaaacatc
     1141 cagtgtgttt tttctgagat tctttggtac ttgagggacc gtgatagcat gcatatcaac
     1201 cacacaatag ttacagtcat attcatcctg caactcagtg aaatttctca atgcacctat
     1261 atagttccct attgtcagtg caccagaagg ctgaacacca ctgaatacaa ttttcttttc
     1321 cataaacaat catcctccta aaatataata acccttcatc tcctatttaa aagagacgaa
     1381 gggtattccg cgttgccact cttcttgcat ttctgccagc ttgattccct ataagggggg
     1441 atacccttgc cctactcatt tcaggcaatg ctccagagtc cattcactgc ttatccccat
     1501 cgaattttca ccaacatcga ctctctgcaa gggtattata cagctactaa tctccatccg
     1561 cttccatata tcatattata ctacaaaaaa tatgattgta aacaccaatc cagactcatt
     1621 tgctgcaact tcatggttta agatgatttt gcagtttagg gaaatattat ctcctgtcct
     1681 cataagcaat cctaaataat tgggtgtagg tacttttgtc catctttctt ggattacttg
     1741 catttgatcc attttcaact gacatttcag caagcctttc aaaatccttg gggtcaatgt
     1801 ccagggagct caatcctggt agtcctattt cctccgaaag gtttcttatg tattcaattc
     1861 ccttctctgc tgcaattttg tcatcattat cccttatccc catggctctg gcaattcttg
     1921 catacttgtg ctcacactcg ggaagattgt actccataac atatggaagc aatatggaat
     1981 tgcacaatcc atgcggggca tcataaagac tgcccaaggc ttccgccata cagtgtacgg
     2041 atgctacatc tgaatgactg aatgacaaac cacccatcag actccccatc atcatcttat
     2101 ccctggcttc caagtcttct ccatcactga ctgctctgga aatgctggag gctatgtact
     2161 ctactgcata gagaccaaaa gcttcagcca agggctctgt gcaattggct gtgtatcctt
     2221 caatggcatg tgtcaaggca tcaattcccg tagctgccgt tatcaaaggt ggtacactga
     2281 tagtcagttc tggatcaaga attgctactt ttgctgctat atgagggctt tttacggtga
     2341 atttgaaatt cctctctgtg tcagttataa cagaagagaa ggtcacttca cttcccgtcc
     2401 cggatgtagt gggtatggtt attaatggca tgcaatcctt cccaatctta tccttgccct
     2461 ggtaatccct tattcttcct ccctgcctgg aaaccacagc ggctcctttt gcagcatcca
     2521 ttgggctacc cccgccaaat gccaccaggg tatcaacttt attatcccta atagtctttg
     2581 cacattcctc cacattgtaa tcctttggat ttgcttcaat ttcatcgaat acaaggtatt
     2641 cgtatccctc agatttaaga attccttcaa gtctgtccaa caaaccagac ctaactattc
     2701 ccttatctgt tattatcata atcctatttg cttcaagctt tttcagttct tctcccagca
     2761 ggttaagcga ccctgctccg tatctaatag tagttggtaa tgtaaaatca aatttatcaa
     2821 tcaagtaaat tcccccattc cttactcata atcatcccca attataggta ttattagctc
     2881 catggacctt tcaaagctat ccctggacac aagtatgtcc gttttaaatg gcgagctgcc
     2941 tgttattatt ctcatatagc tcccagagcc cttgttctta actataaatg gaataccatt
     3001 atcttcaaga atagctgtta tcatgttcat ttcaaattca ctttctattg tccttaaaac
     3061 aactaaatcc aagttatctg acattattta cctccttatt ccatccttga aaagactact
     3121 gaaccctact attgacttct atacttatcc ctgcccactt caagctcatc tatcttaccc
     3181 tgtgccaatt ttgtagtaat tatcgtcgat gagaacacaa aggtcaggcc aattgttatg
     3241 aagtttataa ggttggcctt tgtcacaggt atgagtccgt gtttgaaatc ctttctatat
     3301 gataaataaa ttgttcatct tccgcaatca aatgattgtc ttccacctca aaaaaccctg
     3361 tattggatac caaggccttt ctctcatcct ctgtgataag tatcccctta agattctcca
     3421 tatccagctt ttcaattgtt ggaatcaaat caaacaagaa aat
//
LOCUS       DASRQF010000081         7243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_213438, whole genome shotgun sequence.
ACCESSION   DASRQF010000081 DASRQF010000000
VERSION     DASRQF010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 7243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7243
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213438"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..1844)
                     /locus_tag="VFD79_02055"
     CDS             complement(174..1844)
                     /locus_tag="VFD79_02055"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HZH92861.1"
                     /translation="MKTDVQIAQAANMIPIKDVAANLGIGEDDIVNYGKYKAKVSLDV
                     FKKMKDKPDGKLILVTAINPTPAGEGKTTTNIGLSMGLNKIGKTAITAIREPSLGPSF
                     GVKGGAAGGGYAQVVPMEDINLHFTGDFHAITTANNLISALLDNHIHQGNKLGIDPRR
                     IVWKRVLDMNDRALRSVVVGLGGKPNGMPREDGFDISVASEIMAIFCLAEDLEDLKRR
                     VGEVIVAYKFDGSPVHAKDLNAQGAVALLMKDAIAPNLVQTLENTPALIHGGPFANIA
                     HGCNSMLATKLSLKLADYTVTEAGFGADLGAEKFFDIKARFGDLKPDAAVIVATVRAL
                     KHHGGAKKDELGTENLDQLAKGFENLEKHIENVKKFGVPAVVAVNNFPTDTQAELDFV
                     LNKCEELGATAVLSEVWAKGGEGGAALAKKVVEVVEKGEANFKPLYDVNSSIVEKIET
                     IAKEIYGADGVDFTKGCQKQIANIEKMGFDKMPICMAKTQYSMADDPTLLGRPKGFRI
                     TVRDIKVSKGAGFLVALTGDIMTMPGLPKVPAADNIDILESGEIVGLF"
     gene            complement(2016..3269)
                     /gene="purD"
                     /locus_tag="VFD79_02060"
     CDS             complement(2016..3269)
                     /gene="purD"
                     /locus_tag="VFD79_02060"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009372679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HZH92862.1"
                     /translation="MKVLVIGSGGREHALCWKLAQSNKIEELYCAPGNGGTSLVATNV
                     DIQSNDLERLLEFSRTIGIELVIVGPEEPLVLGITDMFEDEGISVFGPDALGAKLEGS
                     KDFAKDFMVRHNIPTAKYRTYTDYKKALAGLSEFSYPLVIKADGLCLGKGVVICENRE
                     MAQQTLKEVLVDRIFGDEGQRVVIEEFLEGTEASLLCFVSHNRLFPMESAKDYKQIFE
                     DDKGPNTGGVGCYSPSPLFDEEFNRGVRTGILDRIEKGLEEDGHKFTGILFIGFMINE
                     EGPKVLEFNVRFGDPETEVVIPRLKSDLLDIFIHAIVGDLNEDHISWREEACMTVVLT
                     SGGYPGYYKKGHRIHGLPYPDEDILVFHNGTKRQGDHFVSNGGRVLSVTCIGDMATAR
                     KKIYKAIEGISFMDMGYRRDIGLLK"
     gene            complement(3280..4812)
                     /gene="purH"
                     /locus_tag="VFD79_02065"
     CDS             complement(3280..4812)
                     /gene="purH"
                     /locus_tag="VFD79_02065"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HZH92863.1"
                     /translation="MKRALISVYDKRGIIEFAEKLVQMDWEIISTGGTYRLLLENGIK
                     AREIDEITKFPEILDGRVKTLNPRIHGGILFRRDKEDHRRVIEEMGIFPIHMVVNNLY
                     PFEETVTKSGVKMEEAIENIDIGGPSMIRAAAKNFQDVTVVIDPSDYNQVLLELRQEG
                     DTRPETRLELARKVFNYTAYYDALIAQYFNELCQESFPDYLTLPYRKKDQLRYGENPH
                     QEAAFYMDYSQTEGTIAGAEKLHGKELSYNNINDGNGAIAIIKEFDEPAAVAVKHGNP
                     CGAGVGKSIVEAFEKAYASDTESIFGGIVALNREVTKDLAERLSKIFLEIIIAPSFST
                     EALEILMEKKNVRLLQIPEISDSSYTKPMAKQVEGGMLLQDRNSLLLNDDMEIATGRF
                     PEEEELEDMVFAWKIAKHISSNGVVIAKDGATLGIGLGEVNRFWAVEKAIERAGDKVK
                     GSVLASDGFFPFKDSIEALADAGIAAVIQPGGSVRDAEVVKEADLNKMTMVFTGIRHF
                     RH"
     gene            complement(4793..5422)
                     /gene="purN"
                     /locus_tag="VFD79_02070"
     CDS             complement(4793..5422)
                     /gene="purN"
                     /locus_tag="VFD79_02070"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HZH92864.1"
                     /translation="MDRVRIGVLVSGGGTNLQALMDACGEDKIMGEVVLVISNREDAF
                     ALKRALDNGIASKFVSPLEFEGHDYDRELLRLFIEAEVQLVVLAGYLRVLSPLFIEAY
                     RDRIINIHPSLIPKFCGKGFYGERVHRAVLESGEKETGATVHIVDEGTDTGPILLQEI
                     VPVMNDDTVQTLQKRVLKVEHKILVEAVGKICLGIGNENYGGNHEASID"
     gene            complement(5410..6444)
                     /gene="purM"
                     /locus_tag="VFD79_02075"
     CDS             complement(5410..6444)
                     /gene="purM"
                     /locus_tag="VFD79_02075"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HZH92865.1"
                     /translation="MSLTYKDSGVDKEAGYKEVEMIKGFIEKTHIPGVLSHLGGFSGM
                     FQLDISEMKEPVLVSGTDGVGTKLKLAFMMDRHDTIGEDCVAMCANDILCQGARPLFF
                     LDYIATGKLLPEKMANIVEGVANGCVKAGCALVGGETAEMPGFYQEGEYDVAGFVVGA
                     VDKSKIITGAAIQPGDVILGLPSSGLHSNGFSLVRKIVFEKMGYTVDTYVEDLGKTIG
                     EELITPTRIYSDQVLPLLEKFEIKGLSHITGGGFYENIPRMLPEGTRAKVDPAVIRTP
                     EIFNQLQKWGNIAADEMYGTFNMGVGMVLVAAPEEAERIAHHLVEKNHDFYVLGEVIQ
                     GEKGVDLWTE"
     gene            complement(6466..>7243)
                     /locus_tag="VFD79_02080"
     CDS             complement(6466..>7243)
                     /locus_tag="VFD79_02080"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HZH92866.1"
                     /translation="DYIVASETVAFDTMGAELVRDVEPGEIVMIDKDGLTTIHRKVEK
                     RSLCIFELVYLARPDSIIDDKSVYISRSESGKQLYREFPTKADIVIGAPDSGIVAAIG
                     YAEASGIPYTEGIIKNRYVGRTFIEPTQAIREQGVRIKLNALRENIKGKSLVIVDDSI
                     VRGTTIKRTVKMLKDAGAREVHVRISSPPVTHSCHLGVDTPSEENLIASYMSTEEIRE
                     EIGADSLYFISLEGLIKAAGEENGFCRGCFTGNYPVDKVE"
BASE COUNT         1710 a         1965 c         1366 g         2202 t
ORIGIN      
        1 tgttctcaag ctgcaacaca gcctcaaaca agctatctgc ctgcttactc ttaatttttg
       61 agttatagac cattgtatcc tctcccatct ttgatttaat atacttatat attactgtat
      121 aaaagtgaaa ttgtaaacaa aaagctggga ttttcaccca gctaattttt catctagaat
      181 aatcctacta tctcgcctga ctccaggatg tctatgttgt ctgctgcagg aacctttgga
      241 agtcctggca tggtcatgat atctccagtc aaggcaacca ggaagcctgc ccccttggaa
      301 accttgatgt cccttacagt gatcctgaat cctttaggtc tacccaacag ggttgggtca
      361 tctgccatgg agtattgtgt ttttgccatg catattggca tcttgtcaaa gcccatcttc
      421 tcgatgtttg caatctgctt ctggcatccc tttgtaaagt caacaccgtc agctccatat
      481 atttccttgg caattgtttc gattttctcc acgattgatg agtttacgtc ataaagtggc
      541 ttgaagttag cttcgccctt ctctacaacc tcaactacct tcttggcaag tgcggctcca
      601 ccttctccac cctttgccca aacttctgat agaacagctg ttgcaccaag ctcttcacac
      661 ttgtttagta caaagtcaag ctctgcctga gtgtcagttg ggaagttgtt cactgcaaca
      721 acagctggca ctccaaactt cttgacgttc tcgatatgct tctcaaggtt ttcaaatccc
      781 tttgcaagct ggtcaaggtt ctctgttcca agttcgtcct tcttagcccc tccgtgatgc
      841 ttaagagctc ttactgtagc gactattacc gctgcatccg gcttaaggtc accaaatctt
      901 gccttgatgt caaagaactt ctccgcacca aggtcagcac cgaagccagc ctcggtaaca
      961 gtgtagtctg caagcttaag tgatagtttt gtagcaagca ttgagttaca tccatgggct
     1021 atgtttgcaa atggtccacc atggatcaat gcaggtgtat tctcaagagt ctgtacaagg
     1081 tttggagcta ttgcatcctt cattagaagc gcaactgcac cttgagcatt aaggtccttc
     1141 gcatgtactg gacttccgtc gaacttgtag gctacgataa cctctccaac cctcctctta
     1201 aggtcctcaa ggtcttctgc aagacagaat atggccatta tctctgatgc aacagaaatg
     1261 tcaaatccgt cttcccttgg cattccgttt ggctttccgc caagtccaac aactacactt
     1321 ctaagggctc tgtcgttcat gtccagaact ctcttccaaa caattcttct tgggtctatt
     1381 ccaagcttgt tcccttggtg tatgtggttg tctagcaatg ctgaaataag gttgtttgca
     1441 gtggtgattg catggaagtc tcctgtaaag tgaaggttta tgtcttccat tggtaccacc
     1501 tgtgcgtatc ctccacctgc tgctccaccc ttgactccga agcttggtcc aagtgaaggt
     1561 tctctaattg cagttatggc tgttttcccg attttgttaa gtcccattga taggccgatg
     1621 tttgttgtgg tctttccttc tcctgctgga gttgggttga ttgctgtaac aagaatcagc
     1681 tttccgtctg gcttgtcctt catctttttg aatacatcca aggaaacctt cgctttgtat
     1741 ttaccataat taactatgtc atcttctcca atgcctaagt ttgccgctac gtcctttatt
     1801 ggaatcatat tggccgcctg tgctatctga acatctgttt tcaaaaaaat tccccctctt
     1861 ctagtagtat gtccaactct tttatttata cccaaaagag tataaaatta ttgctggaat
     1921 gttagtaatt tggaatggaa aaagctcata ctactagtat tatagcagat atgagctgaa
     1981 tttgaaatat ttttaattgt taaataattt actaatcact tgagaagtcc tatgtccctc
     2041 ctgtaaccca tatccataaa tgaaataccc tcaattgcct tataaatctt tttcctggcg
     2101 gtagccatgt ccccaatgca ggtcacagaa agaaccctgc ctccattgga tacaaaatga
     2161 tccccctgac gttttgtccc attatggaat acaaggatat cctcatcagg atatggcagt
     2221 ccatggatcc tgtgtccctt cttatagtag ccgggataac ccccggatgt gaggacaact
     2281 gtcatgcagg cttcctccct ccagcttatg tggtcttcat tcaagtctcc cacaattgcg
     2341 tggatgaaaa tatccaaaag gtcagatttc agcctgggta ttacaacctc ggtttcaggg
     2401 tctccaaacc ttacattgaa ctccagtacc ttaggccctt cctcatttat cataaagcct
     2461 atgaatagga ttcccgtgaa cttgtgtcca tcctcctcca ggcccttttc aatcctatct
     2521 agaatccctg tccttactcc cctattgaat tcttcatcaa aaagggggct tggagaatag
     2581 caacccacgc ctcctgtgtt gggtcccttg tcatcctcga aaatctgctt gtagtccttt
     2641 gcgctctcca ttggaaaaag cctgttgtgg gatacaaagc atagaaggga tgcctctgtg
     2701 ccctccagaa actcctctat taccaccctt tggccctcgt ctccgaaaat cctgtcaacc
     2761 aggacctcct ttagggtctg ttgggccatt tccctgtttt cgcatattac tacccccttg
     2821 ccgaggcata ggccgtcagc ctttattacc aggggatagg agaattcaga caagcccgcc
     2881 aaggccttct tatagtctgt gtatgtccta tacttggctg ttggaatatt gtgccttacc
     2941 ataaaatcct tggcaaagtc cttgcttccc tcaagcttcg cacccaaggc atcaggaccg
     3001 aagacactga tgccttcatc ctcaaacata tcggttattc ccagaaccaa gggttcctcc
     3061 ggacccacta tcacaagttc tatcccaatg gtccttgaaa actccaatag cctttccagg
     3121 tcattggact ggatgtcgac gttagtggcc acaagtgagg taccaccatt tcctggcgca
     3181 cagtaaagct cctctatctt attggactgg gccagcttcc agcaaagggc atgctccctt
     3241 cctccacttc ctattaccaa taccttcatc taatccctcc taatgtctga aatgcctgat
     3301 tcctgtaaaa accatggtca tcttgttaag gtccgcttcc ttcacaacct ctgcatccct
     3361 tacagatcct cccggttgga tgacagcagc gatacctgcg tccgccaggg cctcaattga
     3421 atccttgaat gggaagaagc catctgaagc cagaaccgat cccttgacct tgtctccagc
     3481 cctttctatg gccttttcaa cagcccagaa cctgttcacc tcacctagtc ctatcccaag
     3541 ggttgcccca tccttggcga ttaccactcc atttgaggat atgtgcttgg caatcttcca
     3601 tgcaaatacc atgtcctcaa gctcctcttc ttccggaaac ctccctgtgg ctatttccat
     3661 gtcgtcatta aggaggaggc tgttcctatc ctgtagtagc atgcctccct caacctgctt
     3721 tgccattggc ttggtgtagc ttgagtctga aatctcagga atctgcagca gccttacatt
     3781 tttcttttcc ataagtattt caagggcctc tgttgaaaat gaaggggcta tgattatttc
     3841 aaggaatatc tttgaaagtc tctccgccag gtcttttgta acttccctgt taagggcaac
     3901 aattcctcca aatatggact ccgtatcgga tgcgtaggct ttttcaaacg cctccacaat
     3961 tgacttccca actcctgcgc cacaggggtt gccatgtttg acggcaactg ctgcaggctc
     4021 gtcaaattcc tttatgattg caatggctcc attgccgtca tttatgttat tataggacaa
     4081 ttccttgcca tggagttttt ctgcccctgc aattgtccct tcagtttgac tgtaatccat
     4141 gtagaaggcc gcctcctgat ggggattttc cccatacctt agctggtcct tctttctgta
     4201 gggcagggtc aggtagtctg gaaacgactc ctggcatagt tcattgaaat actgggcaat
     4261 aagtgcgtca tagtaggctg tgtagttaaa gaccttcctt gccagctcta gccgggtttc
     4321 cggtcttgtg tcaccctcct gcctcagctc cagaagaacc tggttgtagt cagaaggatc
     4381 tattacaaca gtgacatcct gaaagttctt tgccgctgcc cttatcatgg atggaccgcc
     4441 tatgtctata ttctcaattg cctcttccat cttaacgcca cttttggtca ccgtctcctc
     4501 gaaggggtag aggttgttca caaccatatg gattgggaat attcccatct cctctattac
     4561 cctcctgtgg tcctccttgt cccttctaaa aagtattcca ccatgtattc tggggttcag
     4621 ggtcttgacc cttccatcca gaatttcagg aaactttgtt atttcatcta tctcccttgc
     4681 cttgattcca ttttccaaaa gaagtctgta ggtgcctcca gttgatatta tctcccaatc
     4741 catctggaca agtttttctg caaattcaat tattcctctc ttgtcataga cactaatcaa
     4801 tgctcgcttc atgattacct ccgtagtttt catttccaat cccaaggcat attttcccaa
     4861 cagcctctac caggatcttg tgctccacct ttagcaccct cttctgcaat gtctggaccg
     4921 tatcgtcatt catgacggga actatctcct gcaaaagtat gggacctgta tcagtgccct
     4981 catcaacaat gtggacagtt gctcctgtct ctttttctcc ggactccaga acagccctgt
     5041 ggaccctttc tccatagaaa cccttgccac agaattttgg aatcagggat ggatggatgt
     5101 ttatgatcct atccctatag gcttcaataa aaagtggcga aagaaccctc agatagccag
     5161 ccagtaccac aagctgaacc tctgcttcaa taaaaagcct taatagctcc ctgtcatagt
     5221 catggccttc aaattccaag gggcttacaa acttgctggc aattccattg tccaaagccc
     5281 ttttaagggc aaaggcatcc tctctgtttg agattacaag gacaacttcc cccattatct
     5341 tatcttctcc acaggcatcc ataagggcct gaagattggt gcctcctccg gaaaccaaaa
     5401 ccccgatcct tactcggtcc ataggtctac tcccttttct ccctggatga cctctcctag
     5461 tacgtagaag tcatggtttt tctcaacaag atgatgggct attctttctg cctcctcagg
     5521 cgctgccacc aggaccattc ccactcccat attgaatgtc ccatacatct catccgctgc
     5581 aatattcccc cacttttgaa gctggttgaa gatttccggg gttctgatga cagctggatc
     5641 aaccttggcc cttgttcctt caggtagcat ccttggaata ttctcgtaga atcctcctcc
     5701 ggttatgtgg ctaagtccct ttatttcgaa tttttcaaga agaggcagaa cctggtcact
     5761 gtagattctg gtcggtgtta taagctcctc cccaatggtc tttccaaggt cttccacata
     5821 ggtatccacg gtatagccca ttttttcaaa tacaatcttt cttaccaatg aaaaaccgtt
     5881 gctgtgtagc ccgcttgatg gaaggccaag tataacatct ccaggctgga ttgcagctcc
     5941 agtgatgatc ttggacttgt caacagctcc aacaacaaag ccagcaacgt cgtattcacc
     6001 ctcctggtag aaacccggca tttcagctgt ttctccccca acaagggcac agcctgcctt
     6061 gacacagccg ttggcaaccc cctctacaat gtttgccatc ttttcaggca ggagtttgcc
     6121 tgtggcaata tagtcaagaa aaaacaaagg tcttgctccc tgacaaagga tgtcgttggc
     6181 acacattgca acgcagtcct caccaatggt atcgtgccta tccatcataa aggccagctt
     6241 cagctttgta ccaacaccat ctgtacctga aaccagaaca ggctccttca tttcacttat
     6301 atccagttga aacattccgg aaaatccccc aaggtgtgaa agcactccag gaatatgggt
     6361 tttttcaatg aatcccttta tcatctccac ctctttatag ccagcttcct tgtcaacacc
     6421 agaatcctta tacgttaaag acatgtacag tccctcctaa aatttctatt ccaccttgtc
     6481 aacaggatag ttacctgtga agcaacctct acagaatcca ttttcctctc ctgctgcctt
     6541 gatcaggccc tccagggaaa tgaagtacaa tgaatccgca cctatttcct cccttatttc
     6601 ctcagtactc atatagcttg caatcaggtt ttcctcagat ggtgtgtcca caccaaggtg
     6661 acagctgtgg gtcaccggtg gtgatgagat ccttacgtgt acctcccttg ctcccgcatc
     6721 cttgagcatc tttacagtcc tcttgattgt agtgcccctt acaatggagt catccacaat
     6781 taccaggctc ttgcccttga tgttttccct cagtgcgttc agcttgattc taactccctg
     6841 ctccctgatt gcctgagttg gctctatgaa ggtcctgcct acatacctgt tctttattat
     6901 cccttcagta taggggattc cagaggcttc tgcatagcca atggctgcca ctattcctga
     6961 atcaggtgca cctatcacta tgtcggcctt tgtggggaat tccctgtaca gctgtttccc
     7021 agattcagac cttgaaatat aaacactctt atcatcgatt atgctgtcag gccttgcaag
     7081 gtataccagc tcgaagatac aaaggcttct cttctccacc ttcctatgga tagtggtgag
     7141 accatccttg tcaatcatta caatttcacc aggctccaca tctctcacga gttctgcccc
     7201 cattgtatca aaggcaaccg tctcagatgc cacaatatag tca
//
LOCUS       DASRQF010000082         2433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1439272, whole genome shotgun sequence.
ACCESSION   DASRQF010000082 DASRQF010000000
VERSION     DASRQF010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439272"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="VFD79_02085"
     CDS             <1..889
                     /locus_tag="VFD79_02085"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HZH92867.1"
                     /translation="GSFGLIFGSFLMDRLGRDNVEFVMDKDRMERYSGVERIINGKRY
                     DFNLVPGEEKGKPADLIIFAVKSTGLDEAIRTVRNKVSEETIIISLLNGITSEKIIGE
                     AFGKDKLLYTIAEGMDPIRNGHKLDYTRIGQVRIGIDEETEKKKEMLKEVLDIFDRTG
                     FPYTLEEDVMQRLWSKFMLNVGVNQVVMIHEGNYDTVQKPGPARDLMVEAMREVIKLA
                     ERENIKITEADIDYYVALIDSLNPEGMPSMRFDGLNRIRSEVDLFSGMVLELGKKHGL
                     PTPINQEIYGRIKEIEAEY"
     gene            1042..1398
                     /locus_tag="VFD79_02090"
     CDS             1042..1398
                     /locus_tag="VFD79_02090"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZH92868.1"
                     /translation="MDQLTNLFKVLSDGTRLRVVALLYREELCVCELVGVLDMSQPKI
                     SKSLSKLRDLNLVQDERKEKFVFYSLKRDNEILNGIIEGILGNIHDYPQIQQDMERLS
                     EKEKYLDQCNPLSILE"
     gene            1415..2422
                     /locus_tag="VFD79_02095"
     CDS             1415..2422
                     /locus_tag="VFD79_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HZH92869.1"
                     /translation="MAIFSWLNSQLLKMEWLYKLVELLVENVFGMDINERIGGSVHFF
                     IYDVIKIFILLSFLIFAISYIQSFFPPERTRRILGRYKGITANILGALLGTVTPFCSC
                     SSIPLFIGFTSAGLPIGVTFSFLISSPLVDLASVLLLASIFNWRIAIAYVVVGLILAV
                     IGGSIISMAKLEKYVEPFVFGNQMANGEEDELTRRDRVIFAKDQVLDIINKVWLYILI
                     GVGIGALIHNWIPESVISAILGQDKWYSVVLATFVGIPMYADIFGTLPIAEALVAKGV
                     GIGTALSFMMAVTALSLPSLIMLKKVVKTKLLAIFLLIVTIGILIIGYGFNAFGHFFI
                     L"
BASE COUNT          733 a          460 c          607 g          633 t
ORIGIN      
        1 ggggtctttt gggcttatat tcggcagctt cctaatggac aggcttggca gggacaatgt
       61 tgaatttgta atggacaagg acaggatgga gagatactca ggtgttgaga ggataatcaa
      121 cggcaagagg tacgatttta acctggtccc gggagaagaa aaaggcaagc ctgctgacct
      181 aattatattt gcagttaagt caacaggtct tgatgaggca atccggactg tcagaaacaa
      241 ggtatccgag gaaacaataa tcatatctct cttaaatgga ataaccagtg agaagatcat
      301 aggcgaggcc tttggcaagg acaagctcct ttacaccata gccgagggga tggatccaat
      361 cagaaatgga cataaactgg attacacaag gattgggcag gtgaggatag gcatagatga
      421 ggaaacagaa aagaagaagg agatgctcaa ggaggtactt gatatttttg acagaaccgg
      481 attcccctat acccttgagg aggatgtcat gcaaaggctc tggagcaagt ttatgctcaa
      541 tgtaggggta aaccaggtgg tgatgatcca tgagggcaac tacgatacag tacagaagcc
      601 aggtcctgcc agggacctta tggtagaggc catgagagag gtcattaagc tcgctgaaag
      661 ggagaacatc aaaatcacag aggctgacat agactactat gttgccctga ttgattccct
      721 taaccctgaa ggaatgccgt caatgagatt tgatggcctc aacaggataa gatcagaggt
      781 ggacctgttt tctggaatgg ttctggagct tggtaaaaag catggtctac ccactcccat
      841 caaccaggag atatacggga gaatcaagga aattgaggca gaatactaat atgcaggacc
      901 taaacgtcaa tctggaaaag gtttggcgtt tttctttgta aagtttcagt caaaggtatt
      961 gacaggaaga aaaagaggga gtaacatata catatactca taagtgcata tgagaatatg
     1021 acacttgaaa ggagaaaaat tatggatcag ttaacgaacc tttttaaggt cctgtcagac
     1081 ggtacaagac tcagggttgt ggccctgcta tacagggaag aactttgcgt atgcgagctt
     1141 gttggagtcc ttgacatgtc ccagcccaag atatcaaaaa gcctttccaa gttgagagac
     1201 cttaaccttg tccaggacga gcggaaggag aagtttgtct tttacagtct gaaaagggac
     1261 aatgaaatac ttaatgggat aattgaggga atcctgggaa atatccatga ctatccccaa
     1321 attcagcagg acatggaaag actttccgaa aaggaaaagt acctggacca gtgcaatccc
     1381 ctatcaatac tagaatagac ggaggagtga agagatggca attttttctt ggcttaacag
     1441 ccagctgttg aaaatggagt ggctgtacaa acttgtagag ctgctggttg aaaatgtgtt
     1501 tggaatggac ataaacgaaa gaataggtgg aagtgtccac ttcttcatct acgatgtgat
     1561 aaagatattt attcttctat catttcttat ttttgcaata tcatatatcc aaagcttctt
     1621 tccaccggaa aggacaagaa ggatccttgg aagatacaag ggaatcacag caaacatcct
     1681 tggggcattg ttaggaacag tgacaccatt ttgctcctgc tcatcgattc cacttttcat
     1741 tggctttacc agcgctggac ttccaatagg ggtaaccttc tccttcctga tatcatcacc
     1801 tcttgttgac cttgcatcgg tcctgctcct tgcaagcata ttcaactgga ggatagccat
     1861 tgcctatgtt gttgtgggac tgatacttgc agtgatcggt gggtctatca taagcatggc
     1921 aaagcttgag aagtatgttg aacccttcgt gtttggaaac caaatggcca acggtgagga
     1981 ggatgagctt acaagaaggg atagggtaat ttttgcaaag gaccaggtcc tggatataat
     2041 aaacaaggtg tggctctaca tccttattgg ggttggaatc ggagccctta tacacaactg
     2101 gataccggaa agcgtcatat cggcaatact tggacaggat aaatggtact ctgtggtcct
     2161 agcaaccttt gttggaattc caatgtatgc ggacatattc ggaacacttc caattgccga
     2221 ggcccttgtt gcaaagggag ttggaatcgg aacggcccta tcattcatga tggctgtgac
     2281 agccctttcc ctgccttccc tgataatgct caagaaggtg gttaaaacca agcttcttgc
     2341 catattcttg cttattgtga ctatcgggat tctaataatt ggatatggat tcaatgcatt
     2401 tggacacttt ttcatattgt aatcggccaa ttg
//
LOCUS       DASRQF010000083         2788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_713205, whole genome shotgun sequence.
ACCESSION   DASRQF010000083 DASRQF010000000
VERSION     DASRQF010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713205"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VFD79_02100"
     CDS             <1..555
                     /locus_tag="VFD79_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HZH92870.1"
                     /translation="NPFGADHQSSEHDPFLVMPEDSVERRRLAQLGIQKGYEDPFTMD
                     DDKVRFAWDTQKYFSTLDTLCLCQFVWGPSWELYGPDEMVELCKTGIGWETSLYELMQ
                     VGERRINMMRAFNAKEGFTKEDDLLPRKIFTPLKGGPTDGVVLDKESHEHAKNLYYDL
                     AGWDKEKGNPTESTLKRLSLQWML"
     gene            572..769
                     /gene="thiS"
                     /locus_tag="VFD79_02105"
     CDS             572..769
                     /gene="thiS"
                     /locus_tag="VFD79_02105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HZH92871.1"
                     /translation="MKVTCNNKTMEIEEKITLPELLEMLNYRKTVAVFINGKQLLSSQ
                     LDGYRINEGDQIKIIRILGGG"
     gene            786..2009
                     /locus_tag="VFD79_02110"
     CDS             786..2009
                     /locus_tag="VFD79_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZH92872.1"
                     /translation="MLRTTQEIDNMLKKHDSYRNGCLNLIASENFASEKVRSYLTSDF
                     GNRYGCYSTMDPAEREYTGNKFIHQFEMEAHELVKEIFKAKYTDLRPIGGHMAGMSVV
                     LALLEPGDLVIEVSLSDWGHGLVGPMCQVRQFDETINVEYMAFDENRAVDTEKLIEQA
                     KRLKPKMIIFGGSGTLFPEPVRELRPLADQQGFHIAYDASHVTGLIASGMFPNPLEEG
                     ADIMFGSTHKSFPGPQGGFVVSNDKDLMIKVGNTLSPSLVTSHHLNRLPALAASILEM
                     KDFGEEYGRQVVKNSKALAKALAKRGFKVLGEGREYTESHLLLVDIGEYVDFAPAKFL
                     EKANILCSDDFSGNSPEVRIGTPESTRRGMKEEHMEDIAEFFKRLIIDKEDIDMVGKD
                     VEDYARQFTGLDYSY"
     gene            2137..>2788
                     /locus_tag="VFD79_02115"
     CDS             2137..>2788
                     /locus_tag="VFD79_02115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HZH92873.1"
                     /translation="MGLVTSTELFRRAQEGGYAIGAFNVNNMEIIQGIVDAAKEEKSG
                     LILQVSAGARKYANSIYLRKLVEAAIEDSDLDIVLHLDHGENLEIVKQCIDDGFTSVM
                     IDASHYPFEENVRITKEVVDYAHARGVVVEAELGKLAGVEDAVSVAKEDATYTDPDEA
                     VEFVKRTGIDSLAVAIGTSHGAYKFKGEPKLDFERLEKIGKLIPNTPLVPHGASTVL"
BASE COUNT          879 a          514 c          712 g          683 t
ORIGIN      
        1 aatccttttg gtgcagacca ccaatcaagt gagcatgatc cattcctggt tatgccggag
       61 gatagcgtgg aaagaagaag actggctcag ttgggaatcc aaaaaggcta tgaggatcca
      121 tttacaatgg acgatgacaa ggtcagattt gcatgggata ctcaaaaata cttttcaacc
      181 ctggataccc tttgtctttg tcaattcgtt tggggacctt cctgggagct gtatggaccg
      241 gatgaaatgg ttgaattgtg caaaacaggt ataggctggg agacatcact ttatgagctt
      301 atgcaggttg gcgaaagacg aattaatatg atgagagcct ttaatgcaaa ggaaggcttc
      361 accaaggaag acgaccttct acccaggaag attttcacgc cccttaaggg tggacccact
      421 gatggtgttg ttttggataa ggaatcacat gaacatgcca aaaaccttta ttatgatttg
      481 gctggatggg acaaggaaaa gggaaacccc acagaatcga ccctaaagag actgtcccta
      541 caatggatgc tttaattaag gaacttttga tatgaaagtt acatgcaata acaaaactat
      601 ggaaattgaa gaaaagatca cattaccaga gcttctggaa atgcttaact atcggaagac
      661 tgtagcggta ttcatcaacg gaaaacagct gctttcatct caattggatg gttacaggat
      721 taatgaaggc gaccagatta agataattag gatactgggc ggtggttaat atgaggagga
      781 aaaaaatgct aagaacaacg caagaaattg ataatatgct taaaaagcat gattcctaca
      841 gaaatgggtg cttaaacctt attgcatcgg aaaactttgc cagtgaaaag gtaagaagct
      901 acctgaccag tgattttggg aacaggtatg gctgctactc aaccatggac ccagctgaaa
      961 gggagtacac gggcaataag ttcatacacc agtttgaaat ggaagcccat gagctggtaa
     1021 aggagatatt caaggctaag tatacggatt taagacccat aggtggacac atggccggaa
     1081 tgtcggtggt tttggccctc cttgagccag gagacctggt tatcgaagta agcctgtcag
     1141 actggggaca cggactggta ggacccatgt gccaggtaag acaatttgat gagactatca
     1201 atgttgagta tatggccttt gatgaaaaca gggcagttga tacagaaaag ctaatagagc
     1261 aggcaaaaag actaaagcca aagatgataa tattcggtgg atcaggcacc ctgtttccag
     1321 agcctgttag agaacttagg ccactggcag accagcaggg cttccatatt gcatatgatg
     1381 catcccatgt gaccgggctg attgcatcag gaatgttccc taatccactt gaagagggag
     1441 cagacataat gtttggcagt acacacaagt ctttccctgg accacagggg ggctttgttg
     1501 tctcaaatga caaagacctg atgatcaagg taggtaatac cctttcacca tcccttgtca
     1561 ccagtcatca tctaaacaga ttgcctgcct tggcagcttc aatacttgaa atgaaggact
     1621 tcggtgaaga gtatgggaga caggtggtca agaattccaa agcccttgcc aaagccttgg
     1681 ccaagagggg cttcaaggtt ttgggagaag gaagggagta tacagaaagt caccttctat
     1741 tggttgacat tggagaatat gttgactttg cacctgccaa gttcttggaa aaggccaaca
     1801 tactatgttc agatgatttt tccggaaata gtccagaggt aagaattggt acgcctgaat
     1861 cgacaagaag gggcatgaag gaagaacata tggaggatat tgcagagttc ttcaagagac
     1921 ttataattga taaggaagac atcgacatgg ttgggaagga cgttgaagat tacgcaagac
     1981 aattcacagg attggattat tcatattagt acctcccagg gaaaccacac gagagtgtgg
     2041 ttttcccatt gcaaataaag ttattaatgt aaggatattg gggtatactc taatatagag
     2101 tattaggacc aaaataaaca taaaggggag atatatatgg gtctagttac atcaactgaa
     2161 ttgttcagaa gggcgcagga aggtggatac gcaatcggtg cattcaacgt aaacaacatg
     2221 gagataatcc agggtatcgt tgacgctgcc aaggaggaaa agtcagggtt gattcttcag
     2281 gtttccgctg gcgcgagaaa gtatgcaaac tcaatttatc ttaggaagct cgtagaggct
     2341 gcaattgaag atagtgacct ggacatagtt ctgcacctgg accacggtga aaaccttgaa
     2401 attgtaaagc aatgcatcga tgacggattt acctccgtaa tgatcgacgc atcacattat
     2461 ccttttgagg aaaatgtaag aattaccaag gaagtggttg actatgccca tgccagggga
     2521 gttgttgttg aggccgagct tggcaagctt gcaggtgtgg aggatgcagt aagcgtggcc
     2581 aaggaggatg ctacctacac agacccagat gaggcggttg agtttgtcaa gaggacaggc
     2641 attgattcac tggctgtagc aattggtaca agccatggtg catacaagtt caagggtgaa
     2701 ccaaagcttg actttgaacg attggagaaa atcggaaaac ttattccaaa tacacctttg
     2761 gtacctcacg gtgcatcaac tgtacttc
//
LOCUS       DASRQF010000084         2236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_445944, whole genome shotgun sequence.
ACCESSION   DASRQF010000084 DASRQF010000000
VERSION     DASRQF010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445944"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1437
                     /locus_tag="VFD79_02120"
     CDS             <1..1437
                     /locus_tag="VFD79_02120"
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZH92874.1"
                     /translation="PYGSEASYEQAYFAFDDGYMQLKQREWTADRDVLIPLAAWIGAL
                     FLGTVVLLAYQFIVTGKKAGDEEVHTATVDRVYLEILLVGLMLLGLLWFSGISSLLYS
                     GDIGFNGNGAMSSRQTANLYMAVGLTALVTTGVGVILLSVARRIKGKKMIESSFVFGG
                     LRRGLQKGRSFMDGSRFDKSSLTRVLHQRQIYFVAGSLLLVLMTFLMLIVATPLAIIP
                     PIMEGFLIYWYFKYNKETFDEIEKGFDESLEEQMKSERMKINLVTNVSHDLKTPLTSI
                     ISYVDLLSREEEMSPVARDYVRILSEKSERLKNIVSDLFDLARSTSGDIETDFEDLDL
                     KKLVEQTMVDMEDSIEASGFKIRTDLPEEPINIHSDGKKLYRVIQNLLDNALKYSLEG
                     TRIYVELTQDNGKAILRIKNTAGYEMNFSKDEILQRFFRGDQARSGEGSGLGLSIAES
                     FTRVSGGEFDLQIDGDQFNVFMKFPIGI"
     gene            complement(1497..>2236)
                     /locus_tag="VFD79_02125"
     CDS             complement(1497..>2236)
                     /locus_tag="VFD79_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002840521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HZH92875.1"
                     /translation="LRDIETINLELMLSDLELLERRLDKAQKLAKNDKSMRLEVDVIK
                     KALQVLEEGKSLRTLDLSDDEAAVLRSFNMLSSKPIIYIANISEADVGSEVENPYVNQ
                     VKEFALTEGAEVVVVSAEIEEEISQLDEEERKEFLSDLGLSESGLDKLVQASYRLLGL
                     ISFLTVGEQESRAWTIKRDTPAPQAAGKIHSDIERGFIRAEVIHYDDLMAHGGSMASA
                     REKGLVRIEGKDYIMKDGDVVHFRFNV"
BASE COUNT          611 a          485 c          541 g          599 t
ORIGIN      
        1 ccatatggat cagaggcatc atatgaacaa gcctacttcg cctttgatga tggatatatg
       61 cagcttaagc aaagggagtg gaccgccgac agggatgtat tgattcctct tgcagcatgg
      121 attggggcct tgttccttgg aaccgtagtg ctactggcat accagtttat tgtcacaggg
      181 aagaaggctg gggatgaaga ggttcacact gcaactgttg acagggtgta cctggagatt
      241 ctgctcgttg gtctcatgct tctgggactc ctgtggttca gtggtatatc cagccttctg
      301 tattcgggag acataggatt taatggaaat ggtgcaatgt cctcaaggca gacggcaaac
      361 ctctatatgg cggtaggtct aacggccctt gtcactacag gtgtcggagt aattctactg
      421 tcggtagcca gaaggatcaa gggcaagaaa atgatagaaa gcagctttgt atttggagga
      481 ctgagaagag gacttcaaaa gggtaggagc tttatggatg gcagcaggtt tgacaagtca
      541 tcccttacaa gggttctcca ccagaggcag atatattttg ttgcaggaag ccttctcctt
      601 gtactaatga cctttctgat gcttatagta gcaacgcctt tggcgataat accgccaata
      661 atggaaggat ttctgattta ctggtacttc aagtacaaca aggaaacctt tgatgaaata
      721 gaaaagggat ttgatgagag tctggaagag cagatgaagt cagagaggat gaagataaac
      781 cttgtgacaa atgtttccca tgacctgaag actccgctca catcaataat aagttatgtg
      841 gatcttcttt caagggagga ggagatgtcc cctgttgcca gggactatgt aaggattcta
      901 tcagagaaat ctgagagact taaaaatatt gtgtctgacc tctttgacct tgcacggagc
      961 accagtggtg acatagaaac cgattttgag gaccttgacc tgaaaaagct tgtggaacag
     1021 accatggttg atatggaaga tagcatagag gcctcgggtt tcaagataag gacagacctt
     1081 ccggaggagc ctataaacat ccactccgac gggaagaagt tgtaccgggt aattcagaac
     1141 ctgctggaca atgccttgaa gtactccctg gagggtacaa ggatatatgt agagcttact
     1201 caggataatg gaaaggcgat cctcaggatc aagaacactg ctggctatga gatgaacttt
     1261 tccaaggatg agatcctgca gagattcttc cggggagacc aggcaagaag tggagaggga
     1321 agcggcttgg ggctttcaat agctgagagc ttcaccaggg tgtcaggagg agaattcgac
     1381 ctgcagattg acggtgacca gtttaatgtg tttatgaaat tcccaattgg tatttaacaa
     1441 ttaaaatgac ccctgaggtg ataatcacca caggggtcta tttaagttaa aaatatctat
     1501 acattgaatc tgaagtggac aacatctccg tccttcatga tatagtcctt gccctctatt
     1561 ctaacaagcc ccttctccct tgcagaagcc atgcttccac catgggccat caggtcgtca
     1621 tagtgaatta cctcagccct aataaatcct ctttcaatgt cagagtggat ttttccagca
     1681 gcctggggag ctggtgtgtc tcttttgatg gtccaagccc ttgattcctg ttcccctaca
     1741 gtcaagaagc tgatcagacc aagaagcctg taacttgcct gaacaagctt gtcaagtcct
     1801 gactcggaaa gcccaagatc agaaagaaac tcctttcttt cctcctcatc aagctgtgag
     1861 atctcctctt caatttcagc gctgaccaca acaacctctg caccttcagt caaggcaaat
     1921 tccttaacct gatttacgta agggttctca acctctgagc ccacatcagc ctctgagatg
     1981 ttagcaatgt atattattgg ctttgagctt agcatgttga aggatctcaa cactgcagcc
     2041 tcgtcatcac ttaggtcaag tgttctgagt gattttccct cttcaagaac ctggagggcc
     2101 tttttaatca catccacctc aagtctcatg gacttgtcgt tctttgccag tttctgggcc
     2161 ttatccagac gtctttccaa aagctccagg tcagagagca taagctccag gttgattgtc
     2221 tcaatgtccc taagtg
//
LOCUS       DASRQF010000085         2663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1194734, whole genome shotgun sequence.
ACCESSION   DASRQF010000085 DASRQF010000000
VERSION     DASRQF010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194734"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..1277)
                     /locus_tag="VFD79_02130"
     CDS             complement(15..1277)
                     /locus_tag="VFD79_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZH92876.1"
                     /translation="MEKVLIIANEFPPMGGAGVQRTTKFVKYLRDFGFEPLVVTKEHV
                     AQLSDWSLLEDIPRDVEIHRLKPYDTVNREGVLRLPMKFLGTRIMSPDSEYFWYLLNR
                     DRVLDLVKREGIKLVYSTSFPYSSHLMGLYLRRNLPDVKWVADFRDEWTNNPYHRDSI
                     YKRIKFKGEKKMELQVTENCDYFITNTPLMLENFVADNPSLVGRSTYIPNGYDEEDFE
                     GLRNERDGGDKFVITYTGSLYGRRNLDEFMDGLKLAMDRGDVDKKKLEIRIVGNIYDS
                     VIDAYAAKYGLEGQIKSYGYLPHKESIQMLFNSDLILLVIGKGKGSKNFYTGKIFEYI
                     RSDRPILAIVPSDGVAAQVIRETKTGTVVDPEDIEGVAGSLVKYYGQWKQGNLDHSPH
                     WDNVRKYSRKSQTGKLAEIFLGQIQKEK"
     gene            complement(1277..2551)
                     /locus_tag="VFD79_02135"
     CDS             complement(1277..2551)
                     /locus_tag="VFD79_02135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HZH92877.1"
                     /translation="MKDRKITIIGLGYIGLPTAAVMARAGMDVLGYDLNPRVISALNK
                     GEIIIEEPGLSDLVNEMVKAGRLKGVTSLQESDVFIIAVPTPITEDKKADMTYVESAA
                     EEIAPHLKAGDVVVLESTSPPGATEDIVVPILARSGLKIGEEVFVAHSPERVLPGKII
                     QELVQNDRIIGGINPESTGAVKEIYKTFVKGEIYTTDSKTAEMCKLMENTFRDVNIAL
                     ANELAVLSENMGIDAWEVIRYANKHPRVNLHQPGPGVGGHCIAVDPWFVIENHPEGRL
                     IRQARDINDGMPDKVLQKIQELVKDQETKVAILGVTYKPDVDDTRESPILKLVDGLLQ
                     KTKLQVAIHDPHVDREVFPYSGRLYDDIKDAVKDADLIVLGVNHFEYSTLDFGELGNL
                     MKSKKLYDTRNYLNKEELEKVGFHVTVLGRGN"
BASE COUNT          631 a          701 c          498 g          833 t
ORIGIN      
        1 taatttgccc ccttctactt ctctttttgg atttgcccca aaaatatttc agcaagtttt
       61 ccagtctggg attttctgct gtattttcta acattgtccc aatggggact atggtccagg
      121 tttccctgct tccactggcc ataatatttc accagtgacc ctgccacgcc ttctatgtcc
      181 tctggatcca ctacggttcc agttttagtc tccctgatta cctgtgcggc tacaccatca
      241 cttggaacaa ttgcaagtat gggcctgtca gaccgtatat actcgaatat cttcccagtg
      301 tagaagttct ttgaaccctt gcccttgcct ataaccagga gtattaaatc tgaattgaag
      361 agcatctgga tactttcctt gtgtggaagg tatccatagg attttatctg cccttccaga
      421 ccgtacttgg ccgcataggc atctattacg gaatcgtata tattgccaac aatccttatt
      481 tccagtttct tcttgtccac atctcccctg tccatggcaa gcttcaagcc atccatgaat
      541 tcatcaaggt ttcgtctccc gtagagggaa ccggtatagg ttatgacaaa cttgtctcca
      601 ccgtcccttt cattacgcag gccctcaaag tcctcttcgt cgtagccgtt tggaatatag
      661 gtggaccttc caacaaggga tggattgtct gcaacaaagt tctctagcat caggggagtg
      721 ttggtaatga aatagtcgca gttttcagtg acctggagct ccatcttctt ttctcccttg
      781 aatttgatcc tcttatagat gctgtctctg tgataggggt tgttggtcca ttcatcccta
      841 aagtcagcca cccattttac atccggaaga ttccttctta gatagaggcc cataaggtgt
      901 gatgaatatg ggaacgatgt ggaataaaca agttttattc cctctctttt aaccaaatcc
      961 aggaccctgt ccctgttaag gagataccaa aaatattccg aatcaggaga cattattctg
     1021 gttcccagga acttcatggg cagccttagg acaccctccc tatttacggt atcatagggt
     1081 tttagtctat ggatttcaac atcccttgga atatcctcca acagactcca gtcacttagc
     1141 tgggccacat gttccttggt aacaacaagt ggctcaaatc caaaatccct caggtactta
     1201 acaaattttg ttgtcctttg aactccagct ccacccatgg gagggaactc atttgctata
     1261 atcaatacct tttccatcag ttacccctgc ccagtaccgt tacatgaaaa ccaacctttt
     1321 ccagttcttc cttgttcagg taatttctcg tatcgtaaag ttttttactc ttcatcagat
     1381 ttccaagctc accaaaatcc agtgtgctgt attcaaaatg gtttacaccc agaacaatca
     1441 agtccgcatc cttgacagca tcctttatat catcataaag tcttccgctg taggggaaga
     1501 cctccctgtc aacgtgggga tcgtggattg caacctggag tttggtcttt tgaagcaagc
     1561 catccaccag cttaagaatg gggctttccc ttgtatcatc aacatccggc ttgtatgtta
     1621 ctccaagtat tgccaccttt gtttcctggt ccttaacaag ttcctggatc ttctggagga
     1681 ccttgtcagg catgccgtca tttatatccc tggcctgtct aataagtctt ccttcaggat
     1741 ggttctctat tacaaaccac gggtcgacag ctatgcaatg gccccccact ccaggtccag
     1801 gctggtggag attgaccctg gggtgcttgt ttgcatatct gatgacttcc caggcatcta
     1861 ttcccatgtt ctcagaaagg acagccagct catttgcaag tgcaatgtta acatccctga
     1921 aggtattctc cattaatttg cacatctctg cagtctttga atccgttgta tatatttcac
     1981 cctttacaaa ggtcttgtaa atctccttga cggcccctgt ggattctggg tttatccctc
     2041 cgatgatcct gtcgttctgt acaagctcct gaatgatttt acctggaagg actctttcag
     2101 gagaatgggc cacaaatact tcctctccta ttttcagccc agatcttgca agtattggca
     2161 ccacaatatc ctctgtggct cccggtggag aggtggattc aagcactacc acatccccag
     2221 ctttcaggtg gggcgctatt tcttcagctg cactctccac ataggtcata tccgcctttt
     2281 tgtcctctgt tattggagtt ggaactgcaa ttataaaaac atctgactcc tggagagacg
     2341 ttaccccctt aagtcttcct gccttcacca tttcgtttac aaggtcggac aagccaggct
     2401 cttcaattat tatctcaccc ttgttcaagg cactgatcac cctagggtta aggtcatagc
     2461 ccaatacgtc cattccagcc ctggccataa ctgctgccgt aggcaaccca atgtatccaa
     2521 gtccaattat tgtgattttc ctgtctttca ttaatatacc tccaaattgg tccatcctaa
     2581 gacctatcta atattatatg caccacggac catgacaaaa gtcttgtcct ttgttacaaa
     2641 tattcccctt ccatcacttc ttg
//
LOCUS       DASRQF010000086         5673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_569215, whole genome shotgun sequence.
ACCESSION   DASRQF010000086 DASRQF010000000
VERSION     DASRQF010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569215"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..735)
                     /locus_tag="VFD79_02140"
     CDS             complement(253..735)
                     /locus_tag="VFD79_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HZH92878.1"
                     /translation="MSEKLLKALNDQHNYEMLSGYYYLAMASYCESENMKGMSHFLVE
                     QAKEEYEHAMKFYEFINDMDGRVTMQAIPEPKNDYSSFLEVFETALEHEKKVTARIYD
                     LLKLSEEEKSYPTIQFLQWFVEEQLEEENSMKDIIFKLEGVGDHFHGLMMVDRELGSR
                     "
     gene            complement(838..1122)
                     /locus_tag="VFD79_02145"
     CDS             complement(838..1122)
                     /locus_tag="VFD79_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92879.1"
                     /translation="MNDILLVNKINHKDFSGEKQKVFFQIGEMHLPKVEGKLMDLIGG
                     SRLIDDKDVMYKYNGIEVDILTQSIPILVKLLAQNNIPIYGVYAVYNPKL"
     gene            1261..1938
                     /locus_tag="VFD79_02150"
     CDS             1261..1938
                     /locus_tag="VFD79_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZH92880.1"
                     /translation="MSYSMDALSKCILFTDISHEEISGLMEGINYSIKCYSSNDIVAI
                     QGDPCDSLSIILSGDIEIHKPFSSGKIVTISHFTAGNVFAESLVFSDMKTYPATVVSS
                     SDSCVMNIPRVELVQILIRHPKVLENFAGVLSRRIHMLNDRITSLSLDSTRKKIVNIL
                     LLEYGRQKSQYLLLPYSRKKMAELLNIPRPSLSRELIRMKEDGLIDFYKNRIKILNLK
                     NLESEIK"
     gene            2205..3998
                     /locus_tag="VFD79_02155"
     CDS             2205..3998
                     /locus_tag="VFD79_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZH92881.1"
                     /translation="MRQFKKLIPYLRGYRITFVLAMITVTLSQLFYAAGPIILSTAID
                     SIIGNEPVTNPVAMWFMNYLGGREALRGGLWIIGVLIVVNTGLRGGFLYLKGYYSANA
                     SENVSMNLRDKLYDHIQKLPYSYHVKTDTGDLIQRCTSDVETIRRFLGVQLVEVAGSL
                     SMLVFILWSMLSLDRLMTLFSMVLVPVIFGFAYAFFKRIQRDFKEADEAEAKLSTTLQ
                     ENLTGIRVVKAFAREAFELEKFDVRNSTYRISVNRLIDNLAVYWSLSDFLSMFQVGLV
                     IVVGSIMVVQGRITIGILVAFISYVNMLIWPVRQLGRVLTDMGKATVSIDRVDEILSI
                     PQEILIQNGKEPEILGNMEFSNVSFSYDGVKDVLHDISFKVKKGDTVALMGPTGAGKS
                     SLVNLLPRLYDYTSGSVKIDGLELKEIDRHWIRKNIALVLQEPFLYARTIKDNIRFTD
                     PEKQDKSVFQAAKVASIHNDILSFEKKYETMVGERGVSLSGGQKQRMAIARAIITETP
                     IVIFDDSLSAVDTETDMEIRKALEKRRNIATTFVISHRISTVSNADIIIVLDRGKIIQ
                     SGTHKDLIAQEGLYRRIYDIQSSIEEDVLNA"
     gene            4010..>5673
                     /locus_tag="VFD79_02160"
     CDS             4010..>5673
                     /locus_tag="VFD79_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZH92882.1"
                     /translation="MDNITTERQDKIRLGVWRDFYTYLKPYKTQFLKLIVLMVLLGIM
                     DATFPLLSKYAIDEFVRNDSLDGLPFFIGIYLAAVFALSVLILVFIKLAGRLETTIAH
                     DIRRLGFIQLQELPLSYYDNKAVGWLLARMTSDITRLSETISWGIVDLAWGLTIMTGI
                     SVYMIILNPFLAMITLSVVPMLAILSIVFQKKILTAQRSIRKINSQITGAFNEDIQGA
                     KTTKTLVREEKNLEEFSSLTGSMRSQSIKAAILSSVYLPLVIGLGSIGTALAFSFGSG
                     YIMEGIITYGTLVAFISYTAQFFEPIRQMAVIFAEFQSAQASAERVFSLLKEKPEIRD
                     EREIIQRFGDIFQQERDLLPQIDGRIDFENVSFSYQPHEPVLKNFSLTVNPGQTIALV
                     GETGSGKSTIVNLFCRFYEPTEGRILVDGLDYKKMPLQWIHNNLGYVLQSPHLFSGTV
                     KENIRYGKLDATDEEIQEACRIVNAHEFIMELEDGYDTQVGEGGGMLSTGQKQLISFA
                     RAIIRNPSLFVLDEATSSIDTETEKIIQDTITKVLKGRTSFVIAHRL"
BASE COUNT         1681 a         1163 c         1184 g         1645 t
ORIGIN      
        1 cacccctgaa tataggatac gtttttttcg ccctcttcag acaagcccct gagtatgcac
       61 agtcttactg gatgggaaat caatttaagt attgatgcct tctcctctaa aattccctta
      121 tcaaacctca ttcaacccct cctgtttcca tatatttata tattaggata attgaatgta
      181 tttgtcaaga aattatctat atttatattc aaaaaaaaga cacggtccat accgtgcctt
      241 tatggttcag atctatcttg agccaagctc cctatcaacc atcatcagac catggaagtg
      301 gtcccctact ccttcaagtt tgaagatgat atccttcatg ctgttttcct cttccagctg
      361 ctcctctaca aaccactgca gaaactgaat agtgggatag cttttctctt cttcagaaag
      421 cttcaataga tcatagattc ttgctgttac cttcttctca tgctccaatg ctgtttcaaa
      481 cacctcaagg aatgagctgt agtcattctt tggctctggt attgcctgca ttgtaaccct
      541 tccatccata tcatttatga attcataaaa cttcattgcg tgttcatact cctcttttgc
      601 ctgctcaaca aggaaatggg acatgccctt catattctct gattcacaat atgaagccat
      661 tgcaaggtag taataccctg ataacatctc atagttgtgc tggtcattca aagcttttaa
      721 caatttttca ctcataataa tccctccaaa ttttaattga tttcactctc aatagatacc
      781 catatatata gaatctaatc acaaactaaa ttatttgtaa tgattacatt tttaatctta
      841 tagctttgga ttatatactg cataaactcc gtagatggga atgttgtttt gggccagcag
      901 cttaaccagg attggtattg actgggtcag gatgtcaacc tcaattccgt tgtacttgta
      961 catcacatcc ttatcgtcaa tcaatcggct ccctccaatt aggtccatca gcttcccctc
     1021 cacctttgga aggtgcattt ccccaatctg gaagaaaacc ttctgttttt ccccactgaa
     1081 gtctttgtgg tttattttgt taaccagtag tatatcattc atttgcaagc tccttttcct
     1141 atatatctca tcactatata cccctaaatt ttagtgaata acgcaaatgt tattttttgt
     1201 gtgttactta tttcaatggg ggtataaaga acctgttaaa ggaattgagg tgatttgaat
     1261 ttgagttatt caatggatgc cctatcaaag tgcatactat ttacagatat ctcccacgaa
     1321 gaaatatcag gtctaatgga ggggatcaac tactccatca aatgctacag cagtaatgat
     1381 attgtggcaa ttcaaggtga cccctgcgat agcctttcaa tcatactctc gggagatatt
     1441 gaaatacaca agcccttctc atctggaaag atcgttacaa tcagccattt cacagcagga
     1501 aatgtctttg ccgagtctct tgtcttctct gatatgaaga cctatccggc aacggtggtt
     1561 tcatcctcag atagctgtgt tatgaacatt cccagggttg agcttgtaca gatactcata
     1621 agacacccaa aggtccttga aaactttgct ggtgtccttt caaggagaat tcacatgctc
     1681 aatgacagga ttacaagtct ttcactggac agcaccagaa agaaaattgt aaacatactc
     1741 cttctggagt atggaaggca gaagtcccag tatctgctcc ttccatacag caggaaaaaa
     1801 atggcggagc ttttgaatat tcctagacct tctctttcca gagagcttat ccgtatgaag
     1861 gaggatggac ttattgattt ttacaagaac agaataaaaa tcctgaatct gaagaatctt
     1921 gaaagtgaaa ttaaatgaca aaaaataaat gttgacattc caacacttta gtataaaata
     1981 taggggtaag atgaatttta ccagggaggc cataatgatg ataaatctaa aaggacacat
     2041 acaaacaaac ttaacagact tgaaaagtta ctatggccta tacagggaag tcagggttaa
     2101 cctggtttcc atataggctt atgcctatga ttatttaatg aatagaattg aaatgatact
     2161 acagccatag gtttcttgcc tatggctttt tttggaggaa aattatgaga caatttaaaa
     2221 aactaatacc ctatcttaga gggtacagaa ttacatttgt gctggcaatg atcacagtga
     2281 ccctgtctca gctattctac gcagctggcc caataatcct gagcactgca attgattcaa
     2341 taatcggcaa tgagccggtc accaatcctg tagcgatgtg gtttatgaat tatttaggtg
     2401 gcagagaggc actaaggggt ggactctgga taattggagt cctaattgtt gtgaatacag
     2461 gacttagggg tggctttcta tacctgaagg gctactattc ggccaatgct tcagagaatg
     2521 tctccatgaa tctcagggac aagctttatg accatattca aaagctaccc tacagctatc
     2581 atgttaaaac cgacactgga gaccttatcc aaaggtgtac atcggatgtt gaaacaataa
     2641 gaagattcct tggagtccaa ctggttgagg ttgcaggctc actttccatg ctagttttca
     2701 tactgtggtc catgctttcc ctggacaggc ttatgacatt gttttcaatg gttctggtac
     2761 ctgtcatatt tggatttgca tatgccttct tcaagagaat ccaaagggac ttcaaggagg
     2821 ctgatgaagc tgaagccaag ctgtccacca cattgcagga gaacctcact ggcataaggg
     2881 ttgttaaggc atttgcaagg gaagcctttg agcttgaaaa gttcgatgta agaaattcaa
     2941 cctacagaat atcagttaac agacttatag acaatcttgc agtatactgg tcactttcag
     3001 attttctcag catgtttcag gttggtctgg ttattgttgt gggctcgatt atggttgtcc
     3061 agggaaggat cactattgga atccttgtgg ctttcataag ctatgtgaat atgcttattt
     3121 ggcctgtgag acagcttgga agggtcctta cagacatggg aaaggccact gtctccatag
     3181 accgtgttga tgaaatactc agtattccac aggagatatt gatccaaaac gggaaggaac
     3241 cagagatatt gggtaacatg gaattttcca atgtgtcctt ttcctatgac ggggttaagg
     3301 atgtccttca tgatattagt ttcaaggtca aaaaaggtga tactgttgca ttgatgggac
     3361 caactggagc agggaaaagc tcccttgtaa accttctccc aaggctctat gactatacct
     3421 cggggtcggt gaaaatagac ggtttggaac taaaggaaat agacaggcac tggatcagaa
     3481 aaaacattgc cctggtcctc caggagcctt tcctctatgc aaggacaata aaggacaaca
     3541 ttcggtttac agacccagaa aagcaggata agtcagtgtt tcaggcggcc aaggttgctt
     3601 ccattcacaa tgacatactt tcctttgaaa agaaatatga gaccatggtt ggggaaaggg
     3661 gagtctccct ctcaggtggt caaaaacaga ggatggccat tgcaagggcc ataataacag
     3721 aaacacccat tgtgattttt gatgattcac tatctgcagt tgatactgaa actgatatgg
     3781 aaatccgaaa ggccttggag aaacgaagga atatagcgac tacatttgtc atatcccata
     3841 ggatttccac ggtttccaat gctgacatca tcatagtcct ggacagggga aaaatcattc
     3901 agtcaggaac tcacaaggac ctgattgccc aggagggact ttatagaaga atatacgaca
     3961 ttcagtcatc catcgaagag gatgtattga atgcttagga ggaatcacca tggataatat
     4021 cactactgaa agacaagata aaattagatt gggagtatgg agggattttt acacctatct
     4081 taaaccctac aagacccaat ttttgaagtt gatcgttctg atggtcctgc ttggaataat
     4141 ggatgcaaca tttcctctgc tttcaaagta cgccatagac gaatttgtta gaaatgacag
     4201 tcttgatggc cttccattct tcattggcat ctatctggca gcagtgtttg ctttaagtgt
     4261 cctgatactg gtatttataa aattggccgg cagactggag accacaattg cacacgacat
     4321 aagaagactt ggctttatac aacttcagga gctgccactt tcatattatg acaacaaggc
     4381 agtgggatgg ttattggcaa ggatgacttc tgacattacc cgattaagtg aaaccatatc
     4441 atggggaatt gtggaccttg catggggatt gaccataatg acaggaatat cagtctacat
     4501 gataatcctt aatccatttc tggccatgat aaccctgtcg gtggtgccca tgcttgcaat
     4561 tttgagcatt gttttccaga aaaagatcct gacagctcaa aggagtatca gaaagattaa
     4621 ttcacagata accggagcct tcaatgagga tattcaaggg gccaaaacaa ccaagacctt
     4681 ggttagggag gaaaaaaatc tggaagagtt ttcaagcctg accggttcaa tgagaagcca
     4741 gtcaataaag gctgcaattt tatcctcggt ttaccttcca cttgtaatag gtcttggtag
     4801 cataggtacg gcactggcat tttcttttgg ttcaggatat atcatggaag ggatcataac
     4861 ctacggaacc ctggttgcct ttatttccta tacagctcag ttttttgagc ccatcagaca
     4921 gatggcggtt attttcgcag aatttcaaag cgcacaggca tcggcggaaa gggtcttctc
     4981 ccttttaaag gagaaacctg aaatcaggga tgaaagggag atcatacaaa ggtttgggga
     5041 catattccag caggaaagag accttttacc ccaaatagat ggtaggatag attttgagaa
     5101 tgtatccttc tcctatcagc cccatgagcc ggttctcaag aatttcagcc ttacagtcaa
     5161 tcccggtcaa accattgcgc ttgtggggga aactgggtct ggaaagtcaa caatcgttaa
     5221 tctcttctgc aggttctatg aacccacgga ggggcgaata cttgttgatg gtctggacta
     5281 taaaaaaatg ccccttcaat ggatacacaa taacctgggc tatgttctgc aaagtcctca
     5341 cctgttcagc ggtacagtga aggagaatat tcgctacggt aagctggatg caaccgatga
     5401 ggagatccaa gaggcttgta ggattgtaaa tgctcacgag tttataatgg agcttgagga
     5461 tggctacgat acccaggtgg gcgagggagg cggaatgctg tcaaccggtc agaagcagct
     5521 tatctcattt gccagggcaa taatacgtaa tccttcactc tttgtccttg acgaagcaac
     5581 ctcttccatc gatacagaga cggagaagat tatccaggac accatcacaa aagtccttaa
     5641 aggaagaaca agctttgtaa tcgcccacag act
//
LOCUS       DASRQF010000087         3615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_75083, whole genome shotgun sequence.
ACCESSION   DASRQF010000087 DASRQF010000000
VERSION     DASRQF010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75083"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFD79_02165"
     CDS             <1..189
                     /locus_tag="VFD79_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92883.1"
                     /translation="KNVFVVQSDGRNSASLKDGVRYITLTGARISTPDDFANYSYAEF
                     VINGNTVTYEVKTPYKFD"
     gene            complement(255..1280)
                     /gene="mreB"
                     /locus_tag="VFD79_02170"
     CDS             complement(255..1280)
                     /gene="mreB"
                     /locus_tag="VFD79_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HZH92884.1"
                     /translation="MFALRSDVGIDLGTASVLVYIKGKGIVLKEPSVVAIDQYTNKFL
                     AVGEEARKMLGRTPGNIVAIRPLKDGVISDYSITERMLKYFIQKALGRMWFKPRIIIC
                     VPSGITEVEKRAVMEASNEAGASKTYLIEEPIAAAIGAGLDITQPNGNMVVDIGGGTS
                     DVAVISLGGIVVSKSIKTAGDECDEAIIKFIRKKFNMMIGERSAEQLKMEVGCAFPYE
                     EEKVMEVKGRNLLTGLPLNVEVSSEDMLEALHEPVNEIVEAVHEVLEKTPPELASDIS
                     NAGIYLTGGGAMLYGLDKLIEKRTGIETRLADNPVECVAIGTGKSLDSIEVLEKTMKE
                     SDRSRFR"
     gene            complement(1380..2642)
                     /gene="murA"
                     /locus_tag="VFD79_02175"
     CDS             complement(1380..2642)
                     /gene="murA"
                     /locus_tag="VFD79_02175"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008900983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HZH92885.1"
                     /translation="MEKILVEKSPPLKGTVRISGAKNAALPILAATLLGTEDIILEDV
                     PKLKDVQIMCEVLESLGSKVEYLEDNVLKINSANITSYETKYELMSKMRASFLVMGPL
                     LARLGKTKNSMPGGCAIGSRPIDLHLKGFKALGAEIDVDHGDIGAYAERLVGDVIYLD
                     FPSVGATENIMMAATMAEGETVIDNAAMEPEIVDLSNFLNKLGADIKGAGTSTIRIKG
                     VKKLGGAFHQIIPDRIEAGTFMVAAAISGGDVLVDNVINSHLKPVIAKLREAGCTVLE
                     ENDSIRIIADKKLKAIDIKTLPYPGFPTDMQAQFMALMTTLEGTSSVMETVFENRFMH
                     VEELKKMGAQIRIEGRSAILTGVDEIKGAKVEASDLRAGAALVLAGIVADGTTEIGNI
                     YHIDRGYDGIEEKFSNLGAIIYRKTFLD"
     gene            complement(2834..>3615)
                     /gene="wecB"
                     /locus_tag="VFD79_02180"
     CDS             complement(2834..>3615)
                     /gene="wecB"
                     /locus_tag="VFD79_02180"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HZH92886.1"
                     /translation="HVEAGLRSGNLYSPYPEEANRKLTGVMADFHFAPTGKNRENLLK
                     EDYPDEKIFITGNTVIDALKYAVKDDYRFEDERLNNIDFQNKRVILMTSHRRENLGEP
                     MENIFKAVRDAIQDFEDVHVVFPVHLNPKVREIADRILGENPKVSLTEPLDYGPFTNL
                     MARVSLVVTDSGGLQEEAPALGKPVLVIREETERPEGVEAGTAKLVGTSYKGVYDNLL
                     RLLEDQDEYNRMAKAVNPYGDGRASEKIVDILVKKINKGND"
BASE COUNT          842 a          952 c          692 g         1129 t
ORIGIN      
        1 aagaatgtct ttgttgtcca aagtgacgga aggaactccg caagtcttaa ggatggagta
       61 aggtatatca ccctgacagg agccagaata agcactcctg atgactttgc caactattct
      121 tacgcagagt ttgtcataaa tggcaacaca gtaacctatg aagtgaaaac accatataag
      181 ttcgactaaa gcaaacaacc cttgtccatc ttggacaagg gttgttttta agtaaagcat
      241 tacaatattc tgttctatct gaacctggac ctgtccgatt ccttcattgt cttctccagg
      301 acctcgattg agtcaaggga ctttccagtt cctattgcca cacactcaac aggattgtct
      361 gcaagcctgg tttctattcc ggttctcttt tcgattagct tgtcaaggcc atataacatg
      421 gctccgccac ctgtcaggta tatgcctgca ttgctgatgt ctgatgcaag ctcaggcggt
      481 gtcttttcaa ggacctcatg tactgcctcg actatctcgt tgaccggttc gtgaagtgcc
      541 tcaagcatgt cctcagaaga aacctcaaca ttaaggggaa gtccagtaag gagatttctt
      601 ccctttacct ccatgacctt ttcctcttca tatgggaacg cgcagcccac ctccatcttc
      661 agctgctcag cggacctttc acctatcatc atgttgaatt tctttctgat gaattttatt
      721 atggcctcgt cgcactcatc tccagcggtc tttattgact tgctgaccac tattcccccc
      781 agggatataa ccgctacatc agaggtacct cctccaatgt caacaaccat attcccgttg
      841 ggctgggtta tatcaagtcc tgctcctatg gcagcggcga tgggctcctc aataaggtag
      901 gttttgcttg caccggcctc gttgctggct tccataactg cccttttttc aacttcggtt
      961 attccactgg gcacacagat aatgatcctg ggcttaaacc acattcttcc aagtgctttc
     1021 tgaataaaat acttaagcat cctttccgtt atgctgtagt cggaaatgac gccgtccttc
     1081 agtggtctga ttgccacaat gtttccaggt gtccttccaa gcattttcct ggcttcttct
     1141 ccaacagcaa ggaacttatt ggtatactgg tcaattgcaa ccactgatgg ctccttaaga
     1201 acaattccct taccctttat atacacaaga acacttgctg tacccaggtc aattccaaca
     1261 tccgatctca atgcaaacat actcttcctc cttcacaggt ttttctataa aaaatgggtc
     1321 ccttgataac gactcacttc ttctgtcatt aggcgtaccc atagttgtcc cactttaaat
     1381 caatccaaaa atgtcttccg gtagattatc gctcccaagt tgctgaactt ctcctctatt
     1441 ccatcgtatc ccctgtcgat gtgatagatg tttcctattt cagttgttcc atccgcaacg
     1501 attccagcca gcacaagagc tgcccctgcc ctaaggtcag atgcctctac ctttgctccc
     1561 tttatttcat ccacccctgt caggattgcc gacctacctt ctattcttat ctgggctccc
     1621 atcttcttaa gttcctcaac atgcatgaac ctgttttcaa atactgtttc cataacagag
     1681 cttgtacctt ccaaggttgt catcagggcc atgaactggg cctgcatgtc agttgggaat
     1741 ccaggatagg gaagggtctt gatgtctatt gccttcagct tcttgtctgc gattatcctt
     1801 atgctgtcgt tttcctcaag aactgtgcag ccggcttccc tcagctttgc aatcacaggc
     1861 ttaaggtgtg aattgatgac attgtcaacc aatacatctc caccactgat ggcagcagcc
     1921 accataaagg ttcccgcttc aatcctatcg ggtattatct ggtggaatgc ccctccaagc
     1981 tttttgactc ccttgattct gattgttgag gttcctgcac cctttatatc tgctcccagc
     2041 ttgttcagga agtttgaaag gtcaacaatt tcaggctcca tggcagcgtt gtcgattacg
     2101 gtttcacctt ctgccattgt tgcggccatc atgatgtttt cagttgcccc aactgaaggg
     2161 aagtctaggt agatcacatc tccaacaagc ctttcagcat atgcaccaat atcaccatgg
     2221 tccacatcaa tttccgctcc aagtgccttg aagcccttga gatgaaggtc tatgggtctg
     2281 gagcctatgg cacatcctcc aggcatggaa ttcttggtct ttccaagtct ggcaagcaag
     2341 ggtcccataa caaggaagga tgctctcatc ttgctcatta gctcatactt ggtttcatag
     2401 ctggttatat ttgcggagtt gatcttaagg acattgtcct ccagatactc aaccttgctt
     2461 ccaagtgact ccagaacctc gcacatgatc tgtacatcct tgagctttgg cacatcctca
     2521 agtattatgt cctctgttcc caatagagtt gcagccagta tgggcagtgc tgcgttcttg
     2581 gctccgctta ttctaactgt cccctttagg ggtgggcttt tctctaccaa tatcttttcc
     2641 aaaaaatctc ctcctataca aatgttagaa tcaagtgttt atatctatca attatgctat
     2701 cgcattaaag ttttcagaat ttacataatt ccagtaactt ctacattata tcatttatgt
     2761 tacaaaaatg catcaaaaaa cactccattt tcaatgttct ggagtgtttt attccactgg
     2821 ggtggagtga caactaatca ttgcccttgt taattttctt taccaggatg tccacgatct
     2881 tctctgaagc ccttccatcc ccatatggat tgactgcctt tgccatcctg ttatactcat
     2941 cctggtcttc cagaagcctt aaaagattgt cataaactcc cttatagctg gttcccacca
     3001 gcttggccgt acctgcctca actccttcgg gcctttcagt ttcctccctg ataacaagca
     3061 ctggcttgcc cagtgccggg gcctcctcct ggagtcctcc agagtctgta acaacaaggg
     3121 ataccctggc catgagattt gtaaatggtc catagtcaag tggctcagta agggaaacct
     3181 tgggattttc cccaagtatt ctgtcagcta tttccctgac ctttggatta aggtggacag
     3241 gaaataccac atggacatcc tcaaaatcct ggattgcatc ccttactgcc ttgaatatgt
     3301 tctccattgg ctctccaagg ttctctctcc tgtggctggt cataagtatt acccttttgt
     3361 tctggaaatc tatattgttt agtctttcat cctcaaatct gtagtcatcc tttacagcgt
     3421 acttaagtgc gtctattaca gtgttccctg tgatgaatat cttttcatca gggtagtcct
     3481 ccttaagcag gttttcgcgg ttttttccgg tgggtgcaaa gtggaagtct gccattaccc
     3541 ctgtaagctt cctgttggcc tcctcgggat agggtgaata aaggttcccg cttcggagac
     3601 cagcctctac atgtc
//
LOCUS       DASRQF010000088         5271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_940776, whole genome shotgun sequence.
ACCESSION   DASRQF010000088 DASRQF010000000
VERSION     DASRQF010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940776"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VFD79_02185"
     CDS             complement(<1..477)
                     /locus_tag="VFD79_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92887.1"
                     /translation="MRDGYKKIFWGTIIATFSINLGIVQILPAFVGWMVVASGISMLE
                     EDSEPVDYGMVKTLSYILIGSTLVGSIITLFQSGQINPPVPLLFYPMIVMIVELSMFH
                     RIFEAAVRDFTALDKRDLAELYTGKDRAFIMLSGASIILVTVSIFLNLVTVAFFAGM"
     gene            complement(478..1356)
                     /locus_tag="VFD79_02190"
     CDS             complement(478..1356)
                     /locus_tag="VFD79_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92888.1"
                     /translation="MEVNNRLFKGTIILTGILALVSLAAMLQMRLDKPVFLDHYYDLR
                     VHEDGNYRQEFRLTYITNADDRRVVNQVEFPDYPNIRLNATEHGFMSHFPWDSSNQQP
                     PGEIHGRYSVRTVHCQIDGLPIVEEMDGAVVKAARVFFNDGSQMDVPIGELHFYRFGL
                     EDSPFESLSSSGSSDGSSSSRYRAREDVTLLSVKNQMLERFEHRLDLKINDHVIDGID
                     GLEIEQDDHLVVRSKLYPGSDPLDVYTMVDIHPELYYKRADGQIGSMRFYNIDSLYHS
                     FNFMDLYRYLKAREVV"
     gene            complement(1343..1795)
                     /locus_tag="VFD79_02195"
     CDS             complement(1343..1795)
                     /locus_tag="VFD79_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF188 domain-containing protein"
                     /protein_id="HZH92889.1"
                     /translation="MRIFVDADGCPVVQVAIKLAKEFNIPITLVKNYAHVLNDDYAEV
                     ITVDTSADSADYYIANHLSKGDIVVTQDNGLAAMCLSRQAVPINQNGLVIDDSNLITM
                     LSGRHLHRELRSQGIYTSKAKKRKKGSDRAFEDSLRKLLLEVTEGGSE"
     gene            complement(1813..2565)
                     /locus_tag="VFD79_02200"
     CDS             complement(1813..2565)
                     /locus_tag="VFD79_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HZH92890.1"
                     /translation="MDRERLERNRYLLKTTIRDQIDISDNDQNRGLPMPDIQKPIKDG
                     QKVFDLVSPEDLKDLSDMTLADSIGNRKTRRKFDPDHTMSLAELSFLLWATQGVRKSS
                     GTTVLRTVPSAGNRHAFESYLAVFNVQGLDQGIYRYLPLDHRLVLENRVDGLREMVNT
                     ASKGQVFVGSGSVAFIWAAVPYRMEWRYTLAAHKSILLDAGHICQNLYLASEALGYGT
                     CAIGNYSQELADEMIGVDGKEEFVVYLAPVGK"
     gene            2734..3435
                     /locus_tag="VFD79_02205"
     CDS             2734..3435
                     /locus_tag="VFD79_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HZH92891.1"
                     /translation="MSDDRKKSIRQGMVAGSPLILGYFPIAMAFGLLAKNTGLTIMDT
                     SATSMLVYAGASQFMFLDLVRAGVSTGSIILATFLLNLRHMMMSASLSVNLKTDTSGF
                     LPIIGFGITDETFSVLSFNKDKLSLAFILTVNLIAYLSWIGGTAVGYLVGEVIPKALQ
                     DSLGLGLYAMFAALLFPSFKVNKDYLKPALLGAMTYFAIFKTGLLSSGWDIIAGIVIA
                     SGLGVIVMGKEDKDE"
     gene            3428..3733
                     /locus_tag="VFD79_02210"
     CDS             3428..3733
                     /locus_tag="VFD79_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HZH92892.1"
                     /translation="MSRYLPVILGMALVTYLPRLFPLMLLNKLRLRPSMMRFLQLVPY
                     TSLSILIARGILTSDPEMLPAAVGGILAAGALAYWKGNLVLSVLSGIGVSFLLITFI"
     gene            3946..4626
                     /gene="prxU"
                     /locus_tag="VFD79_02215"
     CDS             3946..4626
                     /gene="prxU"
                     /locus_tag="VFD79_02215"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_083760882.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:4153..4155,aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HZH92893.1"
                     /translation="MKKNKAVEEGTEKITEVKKEEQSMVKVGKPAPLFEAPAFYKGQF
                     TNVKLEDYKGKWVVLCFYPGDFTFVUATEVSAVAEKFDEFQELGVEVISMSVDSQFVH
                     KVWNDNELSKMIGKDIPFPMASDSDGYIGKMYGIYDEDSGVETRGRFLIDPDGIIQGF
                     EVLTPPVGRNVKETIRQIKAFQLVRETKGAEVAPSGWKPGKKTLKPSPDLVGNVWKEW
                     KVSEAFED"
     gene            4702..5115
                     /locus_tag="VFD79_02220"
     CDS             4702..5115
                     /locus_tag="VFD79_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HZH92894.1"
                     /translation="MKKFDTELKTLKSKGIRPSYIRVRILNYLLEEKIHPTVDEIYEK
                     LGKEILTLSKTTVYNTLKLLVEKELAKAINIEGNELRYEAERGFHGHFKCQECDSIYD
                     IAVSEIAFMDDVEGYLINDEQVLITGLCPKCNKSE"
BASE COUNT         1496 a         1134 c         1189 g         1452 t
ORIGIN      
        1 catgcctgca aagaaggcaa ctgtaaccag gttaaggaag attgatactg taaccaaaat
       61 aatgcttgct ccgctcagca ttataaaggc cctgtccttg cctgtgtaaa gctcagccaa
      121 atccctctta tccaaggctg tgaagtcccg gacagctgct tcaaaaatcc tgtgaaacat
      181 tgacaattca actatcataa caatcatggg ataaaaaagc aatggaacag gtggatttat
      241 ctgaccactc tggaataggg taataatact accaaccagt gttgaaccta taaggatata
      301 tgaaagtgtt ttcaccatgc cataatcaac tggctcagag tcttcctcca gcattgatat
      361 cccggatgca accaccatcc agcccacaaa tgcaggcagg atttgaacaa tcccaaggtt
      421 gatgctgaag gtggctataa tggttcccca aaatattttc ttgtatccat ccctcatcta
      481 taccacctcc ctggccttga ggtatctgta caggtccatg aaattgaagc tgtgatacag
      541 gctgtcaata ttgtagaacc tcatgcttcc aatttggcca tcagccctct tgtagtaaag
      601 ctccgggtgg atgtccacca tggtatagac atccagtgga tccgagcctg ggtatagctt
      661 tgaccttact actaagtgat catcttgttc tatctccaag ccatcaatac cgtctatcac
      721 atggtcgttt atctttaggt ccagcctgtg ttcaaacctt tcaagcatct gattcttgac
      781 agaaaggagg gtcacatcct cccttgcccg atatcttgaa gagcttgatc catcggagga
      841 gcctgaggat gaaaggcttt caaaggggct atcctcaagt ccgaacctgt agaaatgaag
      901 ctccccaatt ggaacatcca tttggctacc atcattgaag aatacccttg cagccttaac
      961 tactgcacca tccatttctt ccacaattgg caatccatct atctggcaat gtactgtcct
     1021 aacgctgtag cgtccgtgga tttctccagg aggctgctgg tttgaggagt cccaggggaa
     1081 gtggctcata aagccatgct cagtggcatt taaccgtatg ttggggtaat ctggaaactc
     1141 cacctggttt acaacccttc tatcatcggc atttgtaata taggtcagtc tgaattcctg
     1201 gcggtagttc ccgtcctcat ggaccctcag gtcgtagtag tggtcaagaa aaaccggctt
     1261 gtccagtctc atctggagca ttgctgcaag ggacaccagg gccagaatcc ctgtaaggat
     1321 aattgtaccc ttaaatagtc tgttattcac ttccaccttc agtcacctcc aatagcagct
     1381 tcctcaggga atcctcaaat gccctgtcac ttcccttttt cctctttttt gcctttgatg
     1441 tgtagattcc ctggcttctc agttccctgt gaaggtgtct gccggaaagc atggtaataa
     1501 gatttgaatc atcaatcaca agaccgttct ggttaattgg gaccgcctgg cgggaaaggc
     1561 acatggcggc aagtccattg tcctgggtta ccacaatatc accctttgaa aggtgattgg
     1621 caatgtagta atcagcactg tcggcacttg tatcaaccgt tatgacctct gcataatcgt
     1681 cattaagtac atgtgcatag ttctttacca gggtgatggg gatattgaat tccttggcca
     1741 gttttatggc cacctgtacc actggacagc catctgcatc aacaaaaatt ctcatatgtc
     1801 accttctcca atctattttc caactggggc caagtacaca acaaattcct ccttgccatc
     1861 aacaccaatc atctcatcgg caagctcctg gctgtagttt ccgattgcac aggttccata
     1921 gcccagggcc tctgaagcaa ggtagagatt ctggcagata tgtccggcat caagaaggat
     1981 ggacttgtgc gcagccaggg tgtatctcca ttccatcctg tagggaaccg ctgcccaaat
     2041 aaaggctacg gaaccagatc ccacaaaaac ctggcccttt gaggctgtgt tgaccatctc
     2101 cctcagacca tccaccctgt tctcaagaac cagcctgtgg tcaaggggaa gatacctgta
     2161 gattccctgg tccaagcctt ggacattaaa gacagccagg tagctttcaa aggcatgtct
     2221 gttgccagca gagggaacag ttctaagaac cgttgttccc gaactcttcc tcacgccttg
     2281 ggttgcccaa agaaggaagg acagctcagc aagtgacatt gtatggtctg gatcaaattt
     2341 tcttctggtc tttcgattcc caatggaatc agccagggtc atatctgaca agtctttcaa
     2401 atcttcaggt gaaaccagat caaaaacctt ttgaccatcc ttgattggct tttggatatc
     2461 cggcatggga agacccctgt tctggtcatt gtctgatata tcaatctggt ccctaatggt
     2521 ggtctttaaa aggtatctgt ttctttcaag tcgttctcta tccatatcta tccctcctaa
     2581 aattcaacat actaaaagta tagcatactt gacaggcaaa ttgagttaca aacccattgc
     2641 aattcctcaa attgttgaat atggacaaaa agatgttaga aagccgaaat attggttata
     2701 attagtctaa tgcaaatgaa aagaggtttt attatgagcg atgacagaaa aaaatcaata
     2761 agacaaggga tggttgcagg atccccactg atacttgggt attttcccat tgcaatggcc
     2821 tttggacttc ttgccaaaaa tacaggtttg acaataatgg acacctcggc gacctcgatg
     2881 ttggtctatg caggtgcaag ccagttcatg ttcctggatc ttgtcagggc aggtgtaagt
     2941 acgggaagta taatcctggc aacatttttg ctgaatctgc gtcacatgat gatgtccgca
     3001 tccctgtccg taaacctgaa aacggataca tcgggtttcc tacccatcat tgggtttggg
     3061 ataaccgatg aaaccttttc ggtcctttcc ttcaataagg acaagctctc cctagccttt
     3121 atcctgacgg tgaatctcat tgcatatttg tcatggatag ggggaacagc tgttggatac
     3181 ctggtggggg aagtaatccc caaagctctt caggacagct tgggcctagg cctgtatgca
     3241 atgtttgcag ccttgctctt tccttccttt aaggtgaaca aggactatct aaagcctgca
     3301 ctcttggggg ccatgaccta ttttgccata tttaagacag gtctgctttc ctcaggctgg
     3361 gatataatcg cagggattgt gatagcgtct ggcctgggtg ttattgtaat ggggaaggag
     3421 gataaggatg agtagatatt tacctgtaat acttggaatg gcccttgtga catatcttcc
     3481 aaggctattt cccctgatgc ttctcaacaa gctaaggctt agaccatcaa tgatgagatt
     3541 tctacagctt gtcccctaca catcccttag catccttata gcaaggggga tactaacatc
     3601 tgatccggag atgttaccgg cagcggttgg agggatttta gcggctggag ccctggcata
     3661 ctggaagggg aaccttgtcc tgtctgtgct ttcgggcatt ggggtttcat ttttgcttat
     3721 aacatttatc tgatgccaag gtgcctcccc atgtgggagg tataattatt tatgacataa
     3781 tcccataaaa atagtttcca tgaatatgta aatgggtatt tacagtactg actgtgatat
     3841 gttatcatca tagaataatt gtaacgagta caaaaataat ataattacaa ttaaggaggt
     3901 gtccatttga cagaacaagg aaattccgga tgtgcaagac cggaaatcaa gaaaaacaag
     3961 gcagttgagg aagggacaga aaagatcacc gaggttaaaa aggaggaaca gtctatggtt
     4021 aaggtaggaa aaccagcacc actattcgag gcaccagcat tttacaaggg acagtttaca
     4081 aatgttaaat tggaggacta caagggcaag tgggtagtgc tttgcttcta tccaggagac
     4141 ttcacctttg tctgagctac tgaagtttca gcagttgctg aaaaatttga cgaattccag
     4201 gagttaggag tagaggtaat ttcaatgagt gttgatagcc agttcgttca caaggtatgg
     4261 aacgataatg agctttcaaa aatgatcgga aaggatattc cattcccaat ggcatcagac
     4321 tctgatgggt acatcggcaa gatgtatgga atctatgacg aggacagtgg agttgagaca
     4381 aggggaagat tcctgataga cccagatgga ataatccaag gtttcgaggt gcttacacct
     4441 ccagttggaa gaaatgtaaa ggagacaatc agacaaatca aggcattcca gcttgtcagg
     4501 gagaccaagg gagcggaggt tgcaccttca ggatggaagc ctggaaagaa aacccttaag
     4561 ccaagcccag accttgtagg taatgtctgg aaagagtgga aggttagcga agctttcgaa
     4621 gactaattga aaagagttga tttatgccat tgaataggat attatgtaaa tggtataaat
     4681 ttaaattgga gttgttgatt catgaaaaaa ttcgatacgg aactgaaaac tttaaagtca
     4741 aagggaataa gaccgtccta tataagggtg agaattttga attaccttct tgaggaaaaa
     4801 atccatccta cggtggatga aatctacgag aaactgggta aggagatttt gactctttca
     4861 aagactacag tctacaacac cctcaaactt cttgtggaga aggaattggc aaaggctatc
     4921 aatatcgagg gaaatgagct tagatatgaa gcggagcgtg gatttcacgg gcacttcaag
     4981 tgtcaggaat gcgactccat atacgatatt gccgtatcgg agattgcctt tatggatgat
     5041 gttgagggct acctgatcaa tgatgagcag gttcttatca ccggcctgtg tccaaaatgc
     5101 aataagagtg aataaattgt aatgattata acaaaaagat acccttttgg ggtatctttt
     5161 tgtttagaca aacctctccc gggtataaag acttcacggg cgttaattat gaagttaata
     5221 tataggaggt tttttaatgg acaagttaaa gttatcaata gtgtattaca g
//
LOCUS       DASRQF010000089         2835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1520142, whole genome shotgun sequence.
ACCESSION   DASRQF010000089 DASRQF010000000
VERSION     DASRQF010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520142"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="VFD79_02225"
     CDS             <1..1003
                     /locus_tag="VFD79_02225"
                     /inference="COORDINATES: protein motif:HMM:NF021179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2027 domain-containing protein"
                     /protein_id="HZH92895.1"
                     /translation="NDVGGGIVKKLEGGLAFVEDEDGFEIPMPVYELVVVEKGQPSAE
                     ARQVADKVVESRNEDVSEPEAYHQELEEEGHDDFNPRFYLGFIKIGNNQTQEGKLRLY
                     VINDSNYYVTYLISELREDGLMYALHQGILQPNTKLPLEDRLAREFENTFHMQLILFR
                     KGKGYPALDPVSSAVQIKARRFYKDNVFRNNDFFYQPAVLVPVIKNELEQKMDMLTEF
                     DSKAVIGEKEKSEKKPQAKRKSTPELEEVDLHIDELVDTTAGLSNSDIIQIQMDKFHF
                     VMESNAKNKGRKIVFIHGVGNGVLKNEIRKQLERKYKVYYQDASFREYGYGATMVII"
     gene            complement(987..2216)
                     /locus_tag="VFD79_02230"
     CDS             complement(987..2216)
                     /locus_tag="VFD79_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92896.1"
                     /translation="MDYTLLKNGKIVHPDCVFKGDILLNNSRIVTTGENISPPDPETR
                     VIDVGGTWLLPGLIHFKTPLFKIEGSGPSSGIYQALSHGSTFLMDKIKIPKLEDYKTI
                     LEDAGKYCQPIICDFSLHLDAQSCSKMKRDDLAYCFIHKGISSFSMQWKHVHKLLAGD
                     FDSLLQFMAKHQLLLIFCTKAIKESSLVGNQTFFSAYLIKLREAVRHISMTGCPLLIT
                     GVSSMDEVRTIRTIGGGQTYIAVNLCNTELDVPSPVSPELFTEYGRLMNNIILDPPAL
                     KTPTDRQNYIENNISYSFLHELTDDILVDETYMVNLCNMYATLPAKLLGIYPQKGALI
                     AGADADIIVWSPSNGQDVARHGENISILKADIKYLVVSGRLIEGSEHIKPNSLNGRYI
                     FRDPVIDQPYKDSILLP"
     gene            2326..>2835
                     /locus_tag="VFD79_02235"
     CDS             2326..>2835
                     /locus_tag="VFD79_02235"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZH92897.1"
                     /translation="MQLQKLINMTWLLLALSSAVFIGIYEVQKKLALHNNSVLMVLLC
                     TTGISALIFIPPVLLSYSGHISPESFYYIPEITRKEHLQVLIKAAIVLTSWLFSYYSM
                     KYLPITIASPIRATAPVWTLLGALVIYSERLTIEQWIGVLVTFSFFYMFSMAGMREGI
                     SFKSNRWIWF"
BASE COUNT          808 a          537 c          642 g          848 t
ORIGIN      
        1 taatgatgta ggtggtggaa tagtaaagaa acttgaaggt ggactggcct ttgttgagga
       61 tgaagacgga tttgagattc caatgcctgt atacgaactg gtggttgtcg agaaggggca
      121 gccttctgcc gaagccaggc aggttgctga taaggttgta gaaagcagaa atgaggatgt
      181 ttcagagcct gaagcttatc atcaggagtt ggaggaagaa ggtcatgatg atttcaatcc
      241 ccgtttttac cttggtttta taaagattgg aaacaatcag actcaagagg gcaagctcag
      301 attgtatgtg attaatgaca gcaactacta tgtaacatat ttaatttctg aattacgtga
      361 agacggtctg atgtatgccc tgcaccaggg aatattacag ccgaatacaa aattgccgct
      421 tgaagacaga ctggcaagag aattcgagaa taccttccat atgcagttga tactgtttag
      481 gaagggcaag ggctatccag cccttgaccc ggtttcttct gctgttcaga ttaaggcccg
      541 ccgtttttac aaggacaacg tatttcgcaa caacgatttc ttctatcagc ctgctgtttt
      601 ggtaccggtt atcaagaacg aacttgagca gaagatggac atgttgactg agtttgattc
      661 aaaggctgtg ataggagaaa aggaaaaaag tgaaaagaag cctcaggcta aaaggaagtc
      721 aacacccgaa cttgaagagg tggatttaca tatcgacgaa cttgttgata caactgcagg
      781 actgtctaat agtgatatta tacagattca gatggataag ttccactttg tgatggaatc
      841 caatgcaaag aataagggcc ggaagatagt ctttatccac ggagtaggta atggcgtgct
      901 aaagaatgaa atacgaaaac aactggaacg caagtacaag gtatattatc aggacgcttc
      961 tttcagagaa tatggttatg gggccactat ggtaataata tagaatcttt gtatggctga
     1021 tctattaccg gatctctgaa tatatagcga ccgttgagac tgttgggttt gatatgttca
     1081 ctgccctcta ttagccttcc actgaccacc agatacttga tatcagcttt aaggattgag
     1141 atgttctcac catgtcgtgc tacatcctgt ccattggaag gcgaccagac tatgatatcg
     1201 gcatcagctc cggcaatcaa agcgcctttt tgcggatata ttcctagtag ctttgcaggt
     1261 aaggtggcat acatattaca caaattgacc atataggtct catctaccag gatatcatca
     1321 gtcagctcgt gtaggaagga ataagaaata ttgttttcaa tataattctg tctgtctgta
     1381 ggggtcttaa gtgccggtgg atccagaata atgttattca tgagccttcc gtattcagta
     1441 aacaactccg gactaaccgg ggaaggaaca tcaagttcag tattacatag attgactgca
     1501 atataggttt gcccacctcc aattgttctt atagtcctta cttcatccat tgatgatact
     1561 ccggtaataa gtaaggggca tcctgtcatt gagatgtgtc ttacggcttc ccttagtttg
     1621 atgagatagg cggagaaaaa ggtctggttg cccacaagcg atgattcctt aatggctttg
     1681 gtacagaaga ttaggagcaa ctggtgtttt gccataaact gcaggaggct gtcaaagtca
     1741 cctgcaagaa gcttgtgaac gtgcttccac tgcattgaga acgaacttat tcccttatga
     1801 ataaagcagt aagccagatc atcccttttc attttgctgc atgactgagc atccagatgc
     1861 aggctgaaat cacagattat cggttggcaa tactttccgg catcttcaag tatggtcttg
     1921 taatcttcaa gttttggaat cttgatttta tccatcagga aagtacttcc atgtgaaagt
     1981 gcctgatata tacctgaaga ggggccacta ccttcgatct tgaatagtgg agttttgaaa
     2041 tggatcaggc ctggcagtaa ccatgtacca cctacatcaa tcaccctggt ttccggatct
     2101 gggggactaa tgttttctcc tgttgtgaca atgcgactat tgttcaggag tatatctccc
     2161 ttgaagacac aatcaggatg aactatcttt ccgtttttta gtagagtata gtccatgaca
     2221 gcctaaatcc tattaaagat aaggtcattt taaagctttt tcaatattgg atttaagaat
     2281 tattgactat cataaatgtt aaacatgctg gatttgaggc tacatttgca gcttcaaaag
     2341 ttgatcaata tgacctggtt gctgttagcc ctaagttcgg cagtttttat cggaatatat
     2401 gaggtccaga agaagcttgc ccttcataat aactcggtac ttatggtact tctgtgtact
     2461 acaggtatca gtgcccttat ctttattcca ccggtactcc tttcatatag tgggcatatt
     2521 agtccagagt cgttttacta tattcctgag atcacaagaa aggaacattt gcaagtactg
     2581 atcaaagctg ccatagtact gacctcatgg ctgttttcgt actattcaat gaagtatctt
     2641 cctattacaa tagcctctcc aatcagggct accgcaccag tttggacctt gcttggtgcc
     2701 ctggtaatat atagcgagag attgactatt gaacaatgga ttggagtttt ggtgaccttt
     2761 tcgtttttct atatgttctc tatggccgga atgcgtgagg gtatttcatt caagtcaaac
     2821 cgttggatat ggttt
//
LOCUS       DASRQF010000090         6766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_939477, whole genome shotgun sequence.
ACCESSION   DASRQF010000090 DASRQF010000000
VERSION     DASRQF010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6766
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939477"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VFD79_02240"
     CDS             <1..598
                     /locus_tag="VFD79_02240"
                     /inference="COORDINATES: protein motif:HMM:NF017482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HZH92898.1"
                     /translation="KYGKLKTASQLLLNQIPETEAEISYIEHVLVSLENATEVKDLDE
                     IKDELILEGYIQRRSMKHKNKKVKLAGPLRFTSSDGFEILVGRNNRQNEILTMKTAKK
                     EDLWLHTKGIPGSHVIIRTDGRPVPDKTLSEAGILAAYYSKGRNSGSVEVDYTEKRYV
                     RKPKNAKTGLVVYDNQKTIVVNPSAAVVDSIKKGHEEQ"
     gene            complement(648..1181)
                     /gene="pyrR"
                     /locus_tag="VFD79_02245"
     CDS             complement(648..1181)
                     /gene="pyrR"
                     /locus_tag="VFD79_02245"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HZH92899.1"
                     /translation="MIVKAVIMDEKAIARAITRISNEIIERNKGVKELVLVGVKTRGV
                     PFARRISEKIYEIEGEKVPVFTLDISLYRDDLAEIGDQPVVNETFQGEIDGKKVVLID
                     DVIYTGRTVRASLDALIDNGRPKKVQLAVLVDRGHREFPIRPDYVGKNVPTSRSEVVG
                     VKLLETDGKDEVVIMKR"
     gene            complement(1178..2098)
                     /locus_tag="VFD79_02250"
     CDS             complement(1178..2098)
                     /locus_tag="VFD79_02250"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZH92900.1"
                     /translation="MKEIEIYVGEEDNERLDYYLSLELDEVSRTRIQKLVKDQLVKVN
                     GSIKKSSYIVKEGDTISVVFPEPKKLEITPEDIPLEIVFEDSDLAVVNKRHGMVVHPA
                     PGNYSGTLVNALMFHMDNLSSINGIIRPGIVHRLDKDTSGLLVIAKNDKTHRGLSEQL
                     KHHGVYREYVALVHGNIRQDTGTIDAPIGRNQKDRKKMAVTGKNSKEAITHFSVSKRY
                     GKYTLVTLRLETGRTHQIRVHLSHMGNPVVGDPVYSGGKNEFKIERQLLHARRLGFIH
                     PSTNENVEFSCDLPEDFKLILERLDKEAQL"
     gene            complement(2098..2565)
                     /gene="lspA"
                     /locus_tag="VFD79_02255"
     CDS             complement(2098..2565)
                     /gene="lspA"
                     /locus_tag="VFD79_02255"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HZH92901.1"
                     /translation="MAFLLGALIILADQVTKYLSVVYLMDKRPVVLIENLLELHFVKN
                     YGAAFGILQNQRFFFITITSLVLIGMSIYMIKNKNKLTRVAQLAMGFLLGGALGNLID
                     RIRLGYVVDFIKVDLRIYDFPVFNIADIFIVIGTGLLIFIILFDKYEKAMGDA"
     gene            complement(2626..3126)
                     /locus_tag="VFD79_02260"
     CDS             complement(2626..3126)
                     /locus_tag="VFD79_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HZH92902.1"
                     /translation="MITPLDIQNKNFKKTFRGFSPKEVNSFLNEMIDDYERMYKENIE
                     YKDKINMLTDQIRQFQTMEETLKSTLLVAQQAADEVTVNARNKAEIIIENAEDTGRRI
                     IDQSREDVRSIKDEYEHLQKELFIFKTRYESFLKSQLFSLDEYYKSEKKVETIEDNSE
                     DMEVKE"
     gene            complement(3139..3864)
                     /locus_tag="VFD79_02265"
     CDS             complement(3139..3864)
                     /locus_tag="VFD79_02265"
                     /inference="COORDINATES: protein motif:HMM:NF037631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmH/Sll1252 family protein"
                     /protein_id="HZH92903.1"
                     /translation="MKRLLDQIQSSMKNHSIITTDFFDPYERKTAESILNRFQELSYF
                     YDGGMEKAERKCILIYPEYVYEKDLPSAVAYLRIDGQLDDLTHKDYLGAVLGLGITRE
                     KIGDILLYGDFTLIIVKREIKDYILLNFEKAGRQNITISEVDGEDIEYPEMDFVEISK
                     FVSSLRLDSYLSAAYNISRSESRALIKSENVKVNWGMTAKADQTLDAGDVVSVRGKGR
                     TVLHEIGGMSKKGRINLKIRKLV"
     gene            complement(3925..4191)
                     /locus_tag="VFD79_02270"
     CDS             complement(3925..4191)
                     /locus_tag="VFD79_02270"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HZH92904.1"
                     /translation="MYTLLRSVRILFDLLEILIIVRIFVNIFRVPVNNMFGKILYELT
                     EPIMAPARALLDKIGIGRGFIDFSPWVAIIFLRVLYTVILNLVA"
     gene            complement(4202..4633)
                     /gene="sepF"
                     /locus_tag="VFD79_02275"
     CDS             complement(4202..4633)
                     /gene="sepF"
                     /locus_tag="VFD79_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HZH92905.1"
                     /translation="MGNIFDKFKYFIGIDDYEEEEYFVEETADELEAGTAPSTKKFNN
                     KVVNIHTNSNMKIAVHEPLSYEEAPAIIDDLKLRKAIVVNFEQLDTTVKRQIFDFING
                     GLYAVEGKIQKVTKDIFILAPNNIEIDGLKDELKNRGIFPW"
     gene            complement(4644..5330)
                     /locus_tag="VFD79_02280"
     CDS             complement(4644..5330)
                     /locus_tag="VFD79_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HZH92906.1"
                     /translation="MSIKDNLYEIDNKIKLALEKAGRKDEVSLVAVTKTLPVERIIEA
                     LDLGIIDIGENKVQELTEKMGILGNRPRYHMIGHLQTNKVKYIVDNVHLIHSLDRISL
                     AKEIDKRGKASDRILDTLIQVNIAQEDTKFGMMEDEVIPFIEKVLMMENISVKGLMTI
                     APFTEDESVLRNTFSGLYNLGERIKAQNYKELSMDILSMGMTNDFELAIEEGSNMIRV
                     GTGIFGKRNY"
     gene            complement(5346..6596)
                     /locus_tag="VFD79_02285"
     CDS             complement(5346..6596)
                     /locus_tag="VFD79_02285"
                     /inference="COORDINATES: protein motif:HMM:NF024112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZH92907.1"
                     /translation="MKGYQGDIKRKAILFIGFVFLILLALNIFPELFGDNNIYMVPEA
                     YEYKVRVPAQGIVVRDEKLYFANTEGDIFKVVKEGERVRVGQRVADILLLEDTTDLKE
                     ELSQIENILNFYDELLAGNGDDNPLNSNGVDLLVSRLHQQLSEGDYQGINDTKESISL
                     YNETVQNFSGNIEGSGITTEGLNERRDYLLSRISQFNKSIHSLNSGIVSYEVDGWEEE
                     LDVDGINSIEADIFNRVQAEEGKGNNAVFKIIDDYQWYLLIDIADENQVDYEIGDSLD
                     VLITTEDKRDIVEIRMPLVEIVEDREDKFYILRGTSFLEKVFDIRFANVEIIDLKEDT
                     LRVPAAAIVEIDGKTGVKVKEFYGVVTFRPVEVLAMDERYAYVSKGDNNGYIEIGEES
                     FRTISIFSEVIGKPNSVNLGDILN"
BASE COUNT         1876 a         1588 c         1101 g         2201 t
ORIGIN      
        1 aaagtacgga aagttaaaaa cagcaagtca gcttctcctg aatcagatac cagaaaccga
       61 ggccgaaatc tcctatattg agcatgtgct tgttagcctt gaaaatgcaa cggaggtcaa
      121 ggatctggat gagataaagg atgagcttat tcttgagggc tacattcaaa ggagaagcat
      181 gaagcacaaa aataagaaag taaagctggc agggccacta agattcactt cttcagatgg
      241 atttgagatc cttgtaggca ggaacaacag gcaaaatgaa attctaacca tgaagacagc
      301 caaaaaagag gacttgtggc ttcacacaaa gggcatccct ggcagtcatg tcattataag
      361 aactgacggt aggcctgtac cggataaaac cctgtcagag gcaggaattc ttgctgcata
      421 ctacagcaag ggaagaaatt ctggaagcgt cgaggttgat tacacggaaa aaagatatgt
      481 tcgaaagcct aaaaatgcca aaaccggttt agtggtttat gataatcaga aaaccattgt
      541 ggtcaaccca tcagctgcag tggtggatag tattaaaaag ggtcacgagg agcaatagac
      601 tttggcctta taatgaaaag ataggtgatt gccaacaagg cgatcaccta tcttttcatt
      661 attaccactt catccttgcc atccgtttca agtagcttta ctccaacaac ttcgctcctt
      721 gaagttggga catttttacc tacataatca ggtctgattg gaaattccct gtgtcccctg
      781 tcaacaagta cagccagctg aacctttttc ggtctaccat tgtctattag ggcatccaga
      841 gaggccctaa ccgtccttcc tgtgtatatt acatcatcaa ttagtactac cttcttaccg
      901 tcaatctcac cctggaatgt ctcatttaca acaggttggt ctccaatttc cgcaagatcg
      961 tccctgtaga gactgatgtc aagggtgaat actgggacct tctcaccttc aatttcataa
     1021 atcttctccg agatcctcct tgcaaatgga acccctcttg tcttgactcc cacaagaacc
     1081 agttccttaa cgcccttgtt cctctcaatt atttcattgg atatcctggt gattgctctg
     1141 gcaattgcct tttcatccat aatcacagcc ttgactatca taattgagcc tccttgtcca
     1201 atctttccaa aatcagtttg aaatcctctg gcagatcaca ggaaaattcc acattctcat
     1261 ttgtagatgg atgtatgaat cccagccttc ttgcgtgaag tagctgcctc tctattttaa
     1321 actcgttctt tccacctgag taaacgggat ctcccaccac aggattaccc atgtgggata
     1381 ggtgaaccct gatttgatgg gttctgcctg tttcaagcct tagtgttacc agggtgtatt
     1441 taccatacct tttactgaca gaaaagtgtg ttatggcttc cttgctgttc ttgccagtca
     1501 cagccatttt ttttctatcc ttctggtttc taccgattgg tgcatcgatg gtccctgtat
     1561 cctgtctgat atttccatgg accagtgcaa catactcccg atagacacca tgatgtttaa
     1621 gctgctcaga caggcctctg tgggttttgt cattcttggc tatgaccagt agtcctgatg
     1681 tgtccttgtc cagtctgtgg acgattccag gccttataat gccatttatt gacgaaaggt
     1741 tgtccatgtg gaacattaga gcatttacaa gagtgcccga ataattccca ggtgcaggat
     1801 ggaccaccat accatgcctc ttattgacca cagccaaatc actatcttca aagacgattt
     1861 ccaatgggat gtcctctggt gttatttcca gtttttttgg ctctggaaaa actaccgata
     1921 tggtatcacc ttccttgaca atatagcttg atttctttat ggacccattt acctttacca
     1981 gttggtcctt aacaagcttt tggatccttg ttcgggacac ctcgtcaagt tcaagggaaa
     2041 gatagtagtc gagtctctcg ttgtcctctt cacctacata aatctcaatt tccttcacta
     2101 tgcatcaccc atggcttttt cgtacttatc aaacaaaatt atgaatatca gcaatcctgt
     2161 gccaatgact ataaaaatat ccgcaatgtt aaatacagga aagtcataga tcctcaggtc
     2221 taccttaatg aaatccacaa cataccccag cctgattcta tctatcaaat tacccaaggc
     2281 tccacccagt agaaacccca ttgccaattg agctaccctg gttaacttgt tcttgttctt
     2341 aatcatataa atagacatac ctatcaggac aagactagtt attgtaataa agaaaaacct
     2401 ttgattctga agtatcccaa aagcggctcc ataattcttg acaaaatgaa gttccaagag
     2461 gttttcaatc aatactacag gtctcttatc cataagatat acaactgata aatacttggt
     2521 tacctggtct gccaatatga tcaacgcgcc caataaaaat gccaatattc ttcctcctac
     2581 catctttcaa tatacagaac aaaaaggagc tgacgctcct ccattctact cttttacttc
     2641 catatcctct gagttatctt ctatagtctc taccttcttt tcgcttttgt aatactcatc
     2701 cagggaaaat aactgagact ttaaaaatga ttcataccta gtcttaaaaa taaataattc
     2761 cttttgaaga tgttcatact catcctttat tgaccttacg tcttctcttg attggtcgat
     2821 gattctcctg cctgtgtcct ctgcattctc aattattatc tcagccttgt tcctggcatt
     2881 tacagtaacc tcatcagcgg cttgctgagc taccaataaa gtgctcttca aagtttcctc
     2941 catggtttgg aactgtctta tctggtcagt aagcatgttg attttgtcct tgtactcgat
     3001 gttctccttg tacattctct cataatcatc aatcatttca ttcaggaatg agttaacctc
     3061 cttggggctg aaccccctaa aggttttctt gaaattctta ttctgtatat ctaagggtgt
     3121 tatcatttga tcactccatt acactagttt tctgattttc agatttattc tccccttctt
     3181 tgacatgccc ccaatttcgt gaaggacagt cctacccttt ccccttaccg atactacatc
     3241 acctgcatca agggtctggt cagccttggc tgtcatgccc caattgacct ttacgttttc
     3301 agattttata agcgctcgac tttcacttct cgatatgttg taggcagcac ttaggtatga
     3361 gtcaagtctt aatgaagata caaacttgct gatctcaacg aaatccattt cgggatactc
     3421 aatgtcctca ccatcgactt ctgatatggt gatattttgc cttccggctt tttcaaaatt
     3481 cagcaggatg tagtccttaa tctccctttt tacaattatc aatgtaaaat caccgtataa
     3541 aagaatatct ccaatctttt cccttgtgat cccaagcccc aatacagccc ccaaatagtc
     3601 cttgtgagtg aggtcatcca actgaccgtc aattcttagg tatgcgaccg cagatggcaa
     3661 atccttttca tatacgtatt caggatagat aagaatgcac tttctctccg ccttttccat
     3721 acctccatcg taaaaatagg ataattcttg aaacctgttt agtatggact cagcagtttt
     3781 tctttcatag gggtcaaaaa aatcagtagt tatgattgaa tggttcttca tcgatgattg
     3841 aatctggtcc aatagtcttt tcattttaaa ttggacctca ttgtcatgga tgtgattgag
     3901 ataaatatct ttgtccaaca taaattaagc taccaggttt aaaatcactg tgtagagaac
     3961 tcttaaaaaa attatggcca cccatggtga aaaatctatg aagcctcttc ctataccaat
     4021 cttgtccaag agtgctcttg caggagccat gattggctct gtaagctcat aaagtatttt
     4081 gccgaacatg ttgttcactg gcactctgaa aatgttgaca aatattctaa caataataag
     4141 tatttccaat aagtcaaata gtattcttac agatcttaac aacgtataca ttcatttctc
     4201 cttaccaagg gaatattccc ctattcttca attcgtcctt taatccgtca atttctatat
     4261 tgttaggggc aagtataaat atatccttgg taaccttttg tatcttgcct tcaactgcat
     4321 acaatccacc gtttataaag tcaaatatct gtcttttgac agttgtgtcc agctgttcaa
     4381 aattgacaac tattgccttc ctcaatttaa gatcatcaat gattgctggt gcttcttcat
     4441 agctaagagg ttcatgaaca gctattttca tattgctgtt ggtgtggatg ttgaccacct
     4501 tgttattgaa tttttttgtt gaaggtgcag tccctgcttc aagctcatca gcagtttcct
     4561 ccacgaaata ctcctcttcc tcataatcgt cgatacctat gaaatacttg aatttgtcga
     4621 atatattgcc catttcatac ctcctagtag tttcttttgc caaaaattcc tgttcctacc
     4681 ctgatcatat ttgagccctc ttcaattgcc aattcaaaat catttgtcat gcccatggaa
     4741 agtatatcca tgctgagttc cttatagttt tgtgccttga ttctctcgcc aagattatac
     4801 aagccactga aggtgtttct cagaacagac tcatcctctg tgaagggtgc tattgtcatc
     4861 agacccttaa cactgatgtt ttccatcatg agtaccttct ctatgaatgg gataacttca
     4921 tcctccatca ttccaaactt tgtatcctcc tgggctatgt tcacctgaat aagagtatcc
     4981 agtatcctgt cacttgcctt tcctctcttg tctatctcct tagccagaga aatcctgtca
     5041 agtgagtgga taaggtgaac attgtcaact atgtacttaa ccttgttagt ctggagatgg
     5101 ccaatcatat ggtatcttgg tcgattaccc aaaatcccca tcttctcagt cagttcctgg
     5161 accttgtttt caccaatatc aatgattccc aaatcaagtg cctcaattat cctttcaaca
     5221 ggaagtgttt tagttacagc aaccaaactc acctcatctt ttctacctgc cttttcaagt
     5281 gcaagcttga ttttattgtc tatttcatac agattatcct tgatgctaat ttcaaagacc
     5341 tcccatcaat ttaatatatc tcccaaatta actgaattgg gtttccctat aacctcgcta
     5401 aagattgata ttgtcctgaa ggattcttca ccaatttcta tataaccatt attgtcaccc
     5461 ttggacacat aagcatatct ctcatccatg gctagtactt caacaggtct aaaagttaca
     5521 acaccataga attccttgac cttaaccccg gtttttccat ctatttcaac aatggccgca
     5581 gcaggaaccc ttaaagtatc ctcttttaaa tcaattatct ctacatttgc aaatcttata
     5641 tcaaaaacct tctcaagaaa acttgttcct ctcagaatgt aaaatttatc ctctctgtct
     5701 tccacgattt caacaagtgg cattctgatt tcaacaatgt cccttttgtc ttcggttgtg
     5761 attaaaacat ctaaactgtc cccaatctcg tagtccacct gattctcatc agctatatca
     5821 attagaagat accactggta atcatctatg attttgaaaa ctgcattatt gcctttgcct
     5881 tcttcagctt gaactcgatt gaaaatatca gcttcaatgc tatttatccc atccacatca
     5941 agttcttctt cccacccgtc aacctcatag gaaacaatcc ctgaatttag agagtggatg
     6001 ctcttattga actgtgaaat cctagagagc aagtaatctc tcctctcatt aaggccttct
     6061 gttgtgatcc cagacccttc aatgtttcca gagaaatttt gtactgtttc attgtacaat
     6121 gagatggatt ccttagtatc atttatacct tgataatccc cttctgaaag ctgttggtga
     6181 agcctggaaa ccagtaggtc gaccccattg gaattcaatg gattatcatc cccgtttcca
     6241 gcaagcaatt catcataaaa attcaggatg ttttcaattt ggctcagttc ttccttcagg
     6301 tcagtggtat cctcaagtaa aaggatgtct gcaactctct ggcccacacg gaccctctcc
     6361 ccttccttga ccaccttgaa gatgtcacct tctgtgtttg caaaataaag tttttcatcg
     6421 cgtacaacta ttccctgtgc aggaactctt accttgtact cataagcctc agggaccatg
     6481 taaatattat tgtctccaaa caactcagga aaaatgttca atgcaaggag tatcagaaat
     6541 acaaatccta taaacagtat agccttcctc tttatgtctc cctgatatcc tttcattaaa
     6601 gtcacaccta actaaaaaat ttgccatatt tactcattat tctaaattga ttaatactct
     6661 caatcttaag cttaattacc tcattggcat atgtcaccat cttaatcgat tgatcactgt
     6721 gaatcgcacc gattagaatc cctttaaact tttcactgga tttagt
//
LOCUS       DASRQF010000091         3048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_150724, whole genome shotgun sequence.
ACCESSION   DASRQF010000091 DASRQF010000000
VERSION     DASRQF010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150724"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="VFD79_02290"
     CDS             <1..948
                     /locus_tag="VFD79_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92908.1"
                     /translation="SISISNRVNERSYTSHDFIVLVEGPDNYVNYLQLGASDTVTIRN
                     LFIGDYRIRPLDSMNFSISDYESTVSITTSNLDRDISIDYSLENTGWFSDWDYQTVRV
                     MDVDTEDIDFSKIELRSSDLDNRSDEIYLDLEPLIVLVEVQVEALQQPEGEDSSSVAD
                     PVQEVTSEPVEAGLQEEVTQPDAAEVTEGLEPIVKEETELLMPEETEDQASTEDTNPV
                     VVEESGQEEGNQDSDIDTINDDESEPLEEAEPVLEEDNKEIELVEPEAVIPENPLGEP
                     KAEGETETVDDEGEGPGETDLREEITPSTDPEETPVDLI"
     gene            964..1494
                     /locus_tag="VFD79_02295"
     CDS             964..1494
                     /locus_tag="VFD79_02295"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZH92909.1"
                     /translation="MTEKKVNRPLKVLSNVVFAIFMLVMAFLIFITAQSRITGMEPSL
                     FGNRLYVVDSGSMEPALKMNSLIVVKERSPDQIEIGDILTYYGAESTTRVTHRVVEIG
                     PGNEYFITQGDANNVEDAMAVEGEKVIGIVALSIPYVGYVFRFLSSTPGIIFIISVGL
                     IALLAPVLMKRQPKEA"
     gene            1512..1994
                     /locus_tag="VFD79_02300"
     CDS             1512..1994
                     /locus_tag="VFD79_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR04088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SipW-dependent-type signal peptide-containing
                     protein"
                     /protein_id="HZH92910.1"
                     /translation="MKNKFIAFSTMLLVSIMVAGGTFAWFTSSPESSLTKAEMEIVKV
                     EVVGNGLENIGVRNLGTSETYIRVLLVPQWSDHSLSVSNVDIDINTSDWAEEAGYYYY
                     RNKLGVRAETTNIVNDISINGMTPEYEGQSFTVKVVAEGVQSANEAWKDVWGIDSLPF
                     "
     regulatory      2023..2102
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2135..2797
                     /locus_tag="VFD79_02305"
     CDS             2135..2797
                     /locus_tag="VFD79_02305"
                     /inference="COORDINATES: protein motif:HMM:NF023806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TasA family protein"
                     /protein_id="HZH92911.1"
                     /translation="MKKRNMVVMSMMLFVVLLIGGGTFAWFTAQADPIVNEFEAGTMG
                     ILIHEEFEPVDNVNPGDCYEKVIYVENTGTKRAYIRILAEAVFDGGQSTDVLQYGINE
                     SGLDWNWWGGWHFVDGWHLEDDGYFYYSKVVEPGHFTAPLLKCNRICFNGPMMDNDYQ
                     GASLDITINADAIQATHNAVTEVWGVSFPTVRSVEAQLTLRDVEAMIAQEEAAFQALG
                     IQ"
BASE COUNT         1007 a          504 c          761 g          776 t
ORIGIN      
        1 tcaatctcaa taagcaatag ggtcaatgag agaagttaca caagtcatga ctttatagtc
       61 ctggttgaag gaccagataa ctacgtgaat tatctccagc tgggagcttc ggatactgtt
      121 accattagaa atctatttat aggggactat aggataaggc cccttgattc catgaatttt
      181 agcataagtg actatgaaag cactgtcagt atcaccacca gcaatctgga cagagacata
      241 tcaattgact acagccttga aaatacagga tggttttctg attgggatta tcaaacagta
      301 agggttatgg atgttgacac tgaagatatt gattttagta agattgagct tcgatccagt
      361 gatttggaca atcgaagcga tgaaatatat ttagatctgg aaccattaat tgttctggtt
      421 gaggtacagg tggaggcttt gcagcaacca gaaggggaag attcatcatc agtggcagat
      481 ccggtccagg aggtgacttc cgagcctgtt gaagctggtc ttcaggagga agtcacccaa
      541 ccggatgccg cagaagtaac agagggatta gaaccaatag taaaagagga aacagagctt
      601 cttatgcctg aggaaacaga ggaccaagct tccacagaag atacaaatcc tgttgttgtt
      661 gaggaatcag gtcaggaaga agggaatcag gattctgata tcgataccat aaatgatgat
      721 gagagtgagc ctcttgaaga agcggaacct gttctagaag aggacaataa agaaattgag
      781 ttggtagaac cagaggctgt gatacctgag aatccattgg gagaacccaa agctgaaggt
      841 gagacagaga ctgtagatga tgaaggtgaa ggaccgggtg aaactgactt gcgggaggaa
      901 atcacaccat caactgatcc agaagagaca ccggtagatt taatctagtt tggagggatg
      961 accatgacag aaaagaaagt gaatagaccc ttaaaagtat tatcaaacgt tgtatttgca
     1021 atattcatgc tggtaatggc cttcctgatt tttataacgg cacaatcaag gatcacaggc
     1081 atggaaccat cattgtttgg aaacaggctt tatgtagttg atagtgggag tatggagcct
     1141 gcacttaaaa tgaattcact cattgtagta aaggaaagaa gccccgatca aatagaaatt
     1201 ggtgatatcc tgacctatta tggggctgaa tcgaccacca gggtgaccca tagggttgtt
     1261 gaaataggcc ctggaaatga gtacttcata acccagggag atgcaaacaa tgttgaggat
     1321 gcaatggctg tggaaggtga gaaggtgata ggaattgtag cactttcaat tccctatgta
     1381 ggttatgtat tcagatttct aagcagtaca ccagggataa tatttatcat atcagttgga
     1441 ctaattgcct tgctggcacc tgtactaatg aaaagacagc ctaaggaagc ctaaggaaag
     1501 gatggatgta tatgaaaaac aaatttattg ctttctcaac catgctcctg gtatccatca
     1561 tggtggctgg cggaacattt gcttggttta catccagtcc ggagtcttcc ctgaccaagg
     1621 ctgaaatgga aatagtcaag gtggaggtag taggaaatgg cttggagaat atcggtgtca
     1681 ggaaccttgg aaccagtgaa acctatataa gggtcctctt ggtgccccag tggagcgacc
     1741 acagcttatc agtatcgaat gttgatattg atataaatac ctctgattgg gcagaggagg
     1801 caggatacta ttactacaga aataaattgg gagtaagggc ggaaacaaca aacattgtta
     1861 atgatattag cataaatgga atgacaccag aatatgaagg acaaagcttt acagtaaagg
     1921 tcgtagcaga gggagtccaa agtgcaaacg aagcctggaa agatgtatgg ggcattgata
     1981 gcttaccttt ttgatctagg taaataggga ctccgttgaa ataaacttgt ttggcaaact
     2041 tggagaaatc caaggacgca aagccacagg gccttcttat agagggcagc ctagctgcag
     2101 atactcaaag accattttaa aggaggagaa aaaagtgaaa aagagaaaca tggtagttat
     2161 gtcaatgatg ttgtttgtag tattacttat cggcggtgga acatttgctt ggttcacagc
     2221 acaggctgat ccgatagtaa atgaatttga agcaggaacc atgggaattt taatccacga
     2281 agagtttgag ccagttgaca atgtcaaccc tggagactgc tacgagaagg taatctatgt
     2341 tgaaaacaca ggaactaaaa gagcatacat tagaatacta gcagaagctg tattcgatgg
     2401 tggacagagt acagatgtat tgcagtatgg tatcaatgaa tcaggtcttg actggaactg
     2461 gtggggagga tggcattttg ttgatggatg gcatctagaa gatgatggct atttctatta
     2521 cagcaaagtt gttgagccag gtcactttac agcaccacta ctgaaatgta ataggatttg
     2581 cttcaatggt ccaatgatgg ataatgatta tcaaggggca agccttgaca ttacaattaa
     2641 tgcagacgct attcaagcaa ctcataatgc tgtaactgaa gtttggggag tatcattccc
     2701 aactgtaaga tcagttgaag cacagttgac tctaagagat gttgaagcaa tgattgcaca
     2761 agaagaagca gctttccaag ctctgggaat tcaatagtag agtagctagg aataagtaaa
     2821 tgtagagtta aaaccattgt ttacatatag gcggaccgtc cgcctatatg taaaatacat
     2881 agaaaaattg tgagtaaagg gagggagaac aatgaaaaga ctactatcaa gatcaatggt
     2941 atttctaggt gtattcgcat tgataatggg agccatgacc acctttggaa taaatccctc
     3001 ctatgctgaa aaccttaggg taattggcaa taatataggc cttcaagt
//
LOCUS       DASRQF010000092         3467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1806019, whole genome shotgun sequence.
ACCESSION   DASRQF010000092 DASRQF010000000
VERSION     DASRQF010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806019"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..293)
                     /locus_tag="VFD79_02310"
     tRNA            complement(217..293)
                     /locus_tag="VFD79_02310"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(257..259),aa:Arg,seq:tcg)
     gene            446..1108
                     /gene="sdaAB"
                     /locus_tag="VFD79_02315"
     CDS             446..1108
                     /gene="sdaAB"
                     /locus_tag="VFD79_02315"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HZH92912.1"
                     /translation="MKYRSVFDIIGPVMVGPSSSHTAGPVRIGQIARRLFNAQPEKVV
                     VRLYGSFAETYKGHASDVAVIGGILNYETDDPRIPDSLVRAQELGMDVTFVKEEAIPE
                     HPNTLKITMTKGDDKLDISAISIGGGVVGITELNGFPLKLSGENPAILIFHKDVYGTI
                     AGVASTLAANKINISGMNVSRIEKGDVALMTIETDQPVSPNLINAITKMENIIRIITL
                     SE"
     gene            1122..1985
                     /gene="sdaAA"
                     /locus_tag="VFD79_02320"
     CDS             1122..1985
                     /gene="sdaAA"
                     /locus_tag="VFD79_02320"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HZH92913.1"
                     /translation="MFRNVQELVQQAQDQNKKLYEIMIDQESELTEKSRKEVIGMMDK
                     QFQVMKSSAMKGLEGVTSYSGMTGGDAKKMDDYISRGNYLTDPTLLKAVSYAISINEV
                     NASVGLICASPTAGSSGVLPAVVIAVQEKLGLDDEEAMKHLFTAGAFGFVIANNAFIS
                     GAAGGCQAEIGSASAMAAAAAVEMAGGSPSQAAHAMAMALKNMLGLTCDPLAGLVEVP
                     CIKRNAAGASNAIMAAEMALAGVESKVPWDEVISAMYNIGLLMPVSLRETSLAGLAAT
                     ETGKLWKEKLL"
     gene            2098..>3467
                     /locus_tag="VFD79_02325"
     CDS             2098..>3467
                     /locus_tag="VFD79_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HZH92914.1"
                     /translation="MPSFGINEPRRMSIVGFSLVFSYLLSFLFEGSVLYGLMELGSVD
                     TRPLILGAIVAHFIGLFSCGFFAESPKRIGAILRLGLALSVLGTVPFLFSPSPLWIVG
                     ISISGLASGWMVAAWGHFLKSFTPKTERIRSCADVLVLSNLIMIAINLVLAKTSPLVG
                     LYLSLLALMVGASLLLRLPLQVDQEEVMMVDPKTSGKLGKTQRVLFIFITVITINSGL
                     MYQVVNPAFSHLQDLVSWYWAVPYIVAILVVRHYARDSGKPIILYVAMVMMMSSFIAF
                     MLLGRGSVDYLIVDTLMLGSFGIFDLFWWSIIGEMLTYAKNPVRIFGIGLSANVLGVL
                     LGDMVGFGIMSSGLSTAETVVISLAVTGLTLVILPFLNNQLQYLLSSHKYLKENQGSD
                     SGEQAYIVDGSHALSPLTEREREVLAHILEGSSNKEISEKLSISENTVKTHIRNIYSK
                     YGVSKRA"
BASE COUNT          953 a          711 c          831 g          972 t
ORIGIN      
        1 atgatgattt taatgagaaa taatagtaat gtacatattg aaacttcttg ctaggtttgt
       61 ctaatgatga actggatctt aagtatcaaa aactgcaaga gcctcaccca ctttatccta
      121 ggttgaggct cttgttaatg gtatttattt cgttgtggct aattattggc taaaatgaaa
      181 ttacactgtt ctttaaattc tagctaatta tgtaaatggt acgcccagag ggattcgaac
      241 ccccgaccaa ctggttcgaa gccagctact ctatccagct gagctatggg cgcacagttg
      301 ttttggtact acaatagtat aacccctggt aaatcctatg tcaataatta aggactcccc
      361 acttcaataa acaatagtga aaaagcgacc tgtatctgat ataatatgta attaatacaa
      421 aacaaggaat gtgaggtgta aaagaatgaa atatagaagc gtatttgata tcattggacc
      481 ggtaatggtt ggtccatcaa gttcccacac tgcaggcccc gtgcgaattg gacagattgc
      541 caggaggctt tttaatgccc agcctgaaaa ggtggttgta aggctgtatg gatcatttgc
      601 tgagacctac aagggccacg cctcagatgt tgctgtgatc ggagggattc taaactatga
      661 gaccgatgat ccaaggatac cggattccct tgtgagagca caggagcttg gcatggatgt
      721 aacctttgta aaggaggagg ccattccaga acacccaaac acgttgaaga ttacaatgac
      781 aaagggagat gacaaactgg atatatcagc catatccatt ggaggtggag tggttggaat
      841 tactgaactc aatggattcc cacttaagct gtcgggagag aacccagcaa tccttatctt
      901 ccacaaggat gtttacggca caattgcagg agtggcatcc acccttgctg ccaacaagat
      961 caacataagt ggaatgaatg tttcaagaat tgaaaagggg gatgttgcac ttatgacaat
     1021 tgagactgat caaccagttt cccccaatct tatcaatgca attactaaaa tggaaaatat
     1081 catcaggata ataaccctaa gcgaatagag aggtgaaagg aatgttcaga aacgttcaag
     1141 agctggtcca gcaggcacag gaccagaata agaagctgta tgaaataatg attgaccagg
     1201 agtccgaact gacagaaaag agccgcaagg aagtcatagg catgatggac aagcaattcc
     1261 aggttatgaa gagttctgca atgaagggtc tggagggcgt cacctcctat tcaggaatga
     1321 ccggtgggga tgccaagaaa atggatgact acataagcag gggaaactac cttactgatc
     1381 ccaccctgct aaaggctgta tcatatgcca tctcaattaa tgaggtcaat gcttcagtgg
     1441 gtcttatctg cgccagtcca actgccggat catcaggagt gcttcctgct gtggttattg
     1501 cagtacagga gaagctgggc ctggatgatg aggaggctat gaaacacctc tttactgcag
     1561 gtgcctttgg atttgtcata gcaaacaatg ccttcatatc cggagcagcc ggtggatgtc
     1621 aggcagagat tgggtctgca agcgccatgg cagccgcagc ggctgttgaa atggcaggag
     1681 gctctccttc acaggctgca catgctatgg ccatggccct aaagaatatg cttggcctga
     1741 cctgtgaccc acttgcagga cttgttgagg ttccttgcat aaagagaaat gcagctggtg
     1801 catcaaatgc aattatggcg gctgaaatgg ccctggctgg tgtggaatca aaggttccat
     1861 gggatgaggt tatcagtgcc atgtacaaca tcggactact tatgcctgtt tcactgaggg
     1921 aaacctccct ggctggtctt gccgctactg aaactggcaa attgtggaaa gagaaattgc
     1981 tttagaattt tcatggtgtt ataattatat aattgaaatg aatatcaaga gtgctaagtc
     2041 gtggtgattt cattaaatct ccccaggttg gcactctttc tactacggag gtttgagatg
     2101 cccagttttg gaatcaatga acccaggaga atgtcgattg ttggattctc tctggttttt
     2161 tcatatcttt tgtccttcct atttgaaggg agcgttttgt atggtcttat ggaactgggt
     2221 tcggtggata ccagacctct gatactaggt gccatagtag cccatttcat aggtttgttt
     2281 tcctgtggtt tttttgctga atcacccaaa aggatcggag caatcctaag gcttggcctt
     2341 gccctgtcgg ttcttggcac agtccccttc cttttttccc cttcgcctct ttggattgtt
     2401 ggaatttcca taagcggact tgcaagtggg tggatggttg cagcatgggg tcacttccta
     2461 aagtccttta caccaaagac tgaaagaatt aggagctgcg ctgatgttct cgtcctttcc
     2521 aacctgataa tgatcgccat taaccttgtt ttggccaaga cctcccctct tgttggactc
     2581 tacctgtcac tgcttgcatt gatggttgga gcctctctcc tgttgaggct acccttgcag
     2641 gtggaccagg aagaagtaat gatggttgat ccaaaaacca gcgggaagct aggtaaaaca
     2701 caacgggtac tcttcatatt catcactgta atcacaatta attcaggtct tatgtaccag
     2761 gttgtaaatc cggccttttc acatcttcaa gaccttgtca gctggtattg ggcagtcccc
     2821 tacattgtgg ccatcctggt ggtgagacac tatgccagag attcaggtaa gcccatcata
     2881 ctttatgtgg caatggttat gatgatgtct tcctttattg catttatgct ccttggaagg
     2941 ggttctgtgg actacttgat agttgatacg cttatgcttg gttcctttgg aattttcgac
     3001 ctcttctggt ggagcattat cggcgagatg ctaacttatg caaaaaatcc agtgaggatt
     3061 tttgggattg gcctttctgc aaatgtactg ggtgtccttc ttggcgatat ggtgggcttt
     3121 ggaattatgt catcaggtct ttcaactgca gaaacagttg tgatctcact agcagtcact
     3181 ggcttgacat tggtgatact gcccttcctg aacaaccagc ttcagtatct cttgagtagc
     3241 cataaatacc tcaaggaaaa tcaaggatca gattctggtg aacaggcata tatagttgat
     3301 ggttctcatg cactgtctcc acttacagaa agggaaaggg aggtccttgc ccatatattg
     3361 gagggcagct caaacaagga aatttcagaa aagctatcca ttagtgaaaa taccgtcaaa
     3421 acccatataa ggaatatcta ctcaaagtat ggtgtcagta aaagggc
//
LOCUS       DASRQF010000093         4850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_399603, whole genome shotgun sequence.
ACCESSION   DASRQF010000093 DASRQF010000000
VERSION     DASRQF010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399603"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1918
                     /locus_tag="VFD79_02330"
     CDS             <1..1918
                     /locus_tag="VFD79_02330"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HZH92915.1"
                     /translation="FSQASFRIGERAPEDLRAPFSMEDREAYEALVEESLARMEAVYR
                     ISPTVQITAKAKVSGYLDLVRDMKLRNTPSGGSKVDELETVSPLELPTDIHVLAIGSS
                     FSSLNTVEAISEDILTQMLAQGIREDQLSYQLEELEGIVASLNLEEGEQVLVSSILME
                     AMDANEFVDTIATERQMEAIRESIRPVVIEQNELIVEKGETITPEKMRIIQLAQLGEE
                     TSSSWTRYIGIFIQLILPLLAMILYIKYFNPEILQGRLIYLMFTLFMISLILARAMET
                     LSPYLIPSAFNAIIATLLVGPGLAIVTNLVLVYMLYFLWTIPGELAIIMIIGNSAGII
                     LLLHQNQRHRVLLNGIYMGTISLIFYLGYYFLGNITTRDLLLNGGAIMTGGLVSGVLA
                     LGTLPLWENVFKVLTPLKLMELTDPNQPLLRRLFAEAPGTYHHSLIVGNLSEAAAASI
                     GANPLLAKAGAYYHDIGKLKRPSYFKENQFGIDNPHDRLEPIQSTEIIISHWEDGMKL
                     GRETKLPREIMDIIDQHHGNTLMSYFYHKASEGGSTLPEEEFRYGGQKPKSKEATIVM
                     LADSVEAAVRSIKNMNETAIETMVGKIIKGKIDDGQLDQSPITTREINTVKKTFVSVL
                     KGIYHERIEYPEKS"
     gene            1935..2381
                     /gene="ybeY"
                     /locus_tag="VFD79_02335"
     CDS             1935..2381
                     /gene="ybeY"
                     /locus_tag="VFD79_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019106985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HZH92916.1"
                     /translation="MEVLFDNRQDKIKIQKSTEDLLEKVIETSLKLENMSLDYEISVS
                     FVTNDEIRELNSQFRNIDKETDVLSFPFEDEFETGIRILGDIVLSVEKAMDQAEDFGH
                     TVERELAYLTAHSTLHLMGYDHMNDDEKSIMRQKEKAIMKALQIFK"
     gene            2396..3100
                     /locus_tag="VFD79_02340"
     CDS             2396..3100
                     /locus_tag="VFD79_02340"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HZH92917.1"
                     /translation="MKVKELIDSFNYAVSGILSAMKLEKSLRIHYIAAITVIGLSTLF
                     NLTRVEFMILLLTVVLVVVLEMINTAIEKTIDMITKEYHPLARVIKDVSAGAVLIASF
                     NAVIVGYLMFFERLNPFKELVLVKIRNSPIHLTFVALILVALLTVGLKSKFYKGHGTH
                     FQGGVVSGHSAVAFCIATIVSILSENLLIITLVFGLAFLVAESRVEGKIHSAMETVIG
                     GILGILVGILIFQIIG"
     gene            3111..4166
                     /locus_tag="VFD79_02345"
     CDS             3111..4166
                     /locus_tag="VFD79_02345"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HZH92918.1"
                     /translation="MDIRKMITLILVVALGFGLAACRQEVPEAPVETPVEEPVVEPEP
                     EPEPVEEPEPTGIPSPLSGIYAAEELVNGRIISVVFDNHPKAKWQSGLKDAELVYEFP
                     VEGPYTRYLGLYLINSPDSLGPIRSARPYFVTMAWQFDSIFVHVGGSEQAKADVRTLR
                     MAEIDGLTSSNKVFWRMSHKRAPNNMYSSMEVLRGVQEERGYRDTSEFEGFIFDMEDL
                     PRTGENAEDILITYNDENNTRYIYDPDLQKYERYKEGARHVDESDESGLYADNIIIQE
                     NSMRVLDREGRLGISLEGQGEGKFITRGVVQDIKWVKDDRKAKIEYLNENNEEIRLSP
                     GTTWIQVVKPGTPITIE"
     gene            4180..4596
                     /locus_tag="VFD79_02350"
     CDS             4180..4596
                     /locus_tag="VFD79_02350"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HZH92919.1"
                     /translation="MNRKDLINRAIKAKKMVYVPYSGFHVGAAILTEDDEIYDGCNIE
                     IASYSPTICAERTAIFKAVSEGHMKIKAIAVVGDADHTYPCGVCRQVIREFGKDATVI
                     IANSEDDYREYALDQLLPHSFGPEDLESPRKEEKNV"
     gene            4589..>4850
                     /locus_tag="VFD79_02355"
     CDS             4589..>4850
                     /locus_tag="VFD79_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HZH92920.1"
                     /translation="MYKSGFITVVGRPNVGKSTLLNQVIGEKISIISDKPQTTRNKIQ
                     MVYTGDDFQIVFLDTPGIQMPKNILGDYMLKISRTTLEEVDIV"
BASE COUNT         1534 a          934 c         1177 g         1205 t
ORIGIN      
        1 gttttcccaa gccagtttca gaattggtga aagggctccg gaagatctta gggcaccatt
       61 ttcaatggag gacagggagg catatgaagc tcttgttgag gaatccctgg ccaggatgga
      121 ggctgtctac agaatatctc caacggttca aatcacagct aaggctaagg tatcaggata
      181 cctggacctt gtcagggaca tgaagcttag aaacactcca agtggaggtt caaaggtgga
      241 tgaattggaa actgtgagtc cccttgaact acccacagac atacatgtac ttgctatagg
      301 atcatctttt tcaagcctaa acacagttga ggcgatttca gaagatattc tcacccaaat
      361 gctggctcaa ggaataagag aggaccagct tagctatcag ctggaggagt tggagggaat
      421 agtcgcttca ttgaatctgg aggagggtga gcaagtccta gtctccagca tacttatgga
      481 ggcaatggat gcaaatgagt ttgttgacac cattgcaaca gaaagacaga tggaagcgat
      541 cagggaaagc atcagacctg tggtgatcga gcaaaacgaa ctgatagtgg aaaagggaga
      601 gaccattaca cctgaaaaaa tgaggattat ccaattggct caacttggcg aggaaacatc
      661 cagttcctgg acaagataca taggaatatt tatccagctg atcttgccct tgctagccat
      721 gatcctttac attaagtact tcaatcctga aatactccag ggaagactga tttacctcat
      781 gttcaccttg tttatgataa gtcttatcct tgcaagggca atggagacct tgtcaccgta
      841 tttgattcca tcggccttca atgccataat agccactctc cttgttggac ctggactggc
      901 catagtcacg aacctggtac tggtctatat gctctatttt ttatggacaa tacctgggga
      961 acttgcaata atcatgataa tcggaaattc agccgggatc atcctgctct tgcaccagaa
     1021 tcaaaggcac agagtgctcc ttaatggcat atacatgggt acaataagcc ttatcttcta
     1081 tctgggatat tattttttag ggaatatcac taccagggac ctcctattaa acggtggtgc
     1141 aatcatgact ggaggcttgg tttccggagt ccttgcactt ggaacactgc ccctttggga
     1201 aaatgtattc aaggtattga caccattgaa gcttatggaa cttacagacc caaaccagcc
     1261 tttgcttagg agactcttcg cagaggcacc aggcacctac caccacagcc tcatagtggg
     1321 caacctttca gaggccgctg ctgcatcaat aggcgcaaac cctcttctgg ctaaggcagg
     1381 agcctactac cacgacatag ggaagttgaa gcgaccttcc tatttcaagg agaaccaatt
     1441 tgggatcgac aatccccatg acaggcttga gcctatacaa agtacggaaa tcattatctc
     1501 ccattgggaa gacggcatga aattgggccg tgaaaccaag ctcccaagag aaataatgga
     1561 tataatagac cagcatcatg gcaacaccct aatgtcatat ttttaccata aagcttcgga
     1621 agggggatcc accttgccag aggaagaatt caggtatggt ggtcaaaaac caaaatccaa
     1681 ggaagctaca atagtgatgc tggcagattc tgtagaggct gcagttagaa gcatcaagaa
     1741 catgaatgaa acagcaatag aaacaatggt tggtaagatt ataaagggaa agattgatga
     1801 cgggcagctg gaccaatccc ccataaccac aagggaaata aataccgtaa aaaagacctt
     1861 tgtttcagtt ctaaaaggaa tataccacga aagaatcgag tatcctgaaa agtcataatt
     1921 atgaggagaa aaaaatggaa gtcctatttg acaatagaca agacaagatc aaaatacaaa
     1981 aatctactga agacttgctg gaaaaggtaa tagagacaag cttgaagctg gaaaacatgt
     2041 cattggatta tgaaatcagt gtttcttttg ttacaaatga tgaaatcaga gagttgaaca
     2101 gtcaattcag aaacatagac aaggaaacag atgttttgtc atttccattt gaggatgagt
     2161 ttgagactgg aatcagaatt ctcggtgaca tagtcctgtc agttgaaaag gccatggacc
     2221 aggcagaaga ttttggccat acggttgaac gggagcttgc ctatcttaca gcccatagca
     2281 ctcttcacct catgggctac gatcatatga atgacgatga gaaatcaata atgagacaaa
     2341 aagaaaaggc aataatgaag gcactccaga ttttcaagta gacgggggga ttttgatgaa
     2401 ggtcaaggaa ctaatagaca gcttcaatta cgcagtatca gggatattat ccgccatgaa
     2461 gcttgagaaa agtttaagga tacattacat tgccgccatc actgtaatcg gtcttagcac
     2521 attgtttaac ctgacaaggg tggagttcat gattttgctc cttacggttg tactggttgt
     2581 agtgcttgaa atgataaaca ctgccataga aaagaccatc gacatgataa ccaaggaata
     2641 ccaccccttg gctagggtca taaaggatgt gtctgcagga gcagttctga ttgcttcctt
     2701 caatgctgta atagtggggt acctaatgtt ttttgaaagg ctaaatccat tcaaggagct
     2761 tgttttggta aagatcagga attctccaat acatctcaca tttgttgcct tgatccttgt
     2821 tgcccttctt acagttggat tgaaatcaaa gttttataag ggtcacggaa cacactttca
     2881 gggtggagtg gttagtggac actcggcggt tgcattttgc atagctacaa tagtgtcaat
     2941 tttatctgag aacctactta taataaccct ggtttttgga ttggcatttc tggttgcaga
     3001 aagccgggtt gagggtaaga tacactcggc catggagaca gtcataggtg gcattcttgg
     3061 tatactggtt ggtatactaa tatttcagat aatcggatag gaggaaggaa atggacataa
     3121 gaaagatgat taccctgatc ttggtggtgg ccctggggtt tggccttgct gcctgcaggc
     3181 aggaggtgcc ggaagcacct gttgaaacgc cggtagagga gcctgttgtc gagcctgaac
     3241 cggaaccgga acctgtagag gagccggagc ctacagggat accatctccc ttaagcggaa
     3301 tttatgcagc tgaggaattg gtaaatggtc gaatcatatc agttgttttt gacaaccatc
     3361 caaaggcaaa atggcagtcg ggattaaagg atgcggagct tgtgtacgag tttccagttg
     3421 aaggacccta taccaggtac cttggactat atctaataaa ctcgccggat tccttggggc
     3481 caattaggag tgcaagacca tactttgtca ctatggcttg gcaatttgac tcaatttttg
     3541 tacacgttgg aggcagtgaa caggcaaaag ccgatgtaag aacccttaga atggcagaaa
     3601 ttgatggcct aacaagctca aacaaggttt tctggaggat gtcccacaag agggcgccta
     3661 acaatatgta ctccagcatg gaagtcctaa ggggtgtgca ggaggagcgt ggatacaggg
     3721 ataccagtga gtttgagggc tttatcttcg atatggagga cttgcccaga acaggggaaa
     3781 atgcagaaga tatcctgatt acatacaatg acgaaaacaa caccaggtat atttatgacc
     3841 cggaccttca aaagtacgaa agatacaagg aaggtgctcg acatgtggat gaatcagatg
     3901 aaagtggact ttatgcagac aatattataa tccaggagaa ttccatgcga gtcctggata
     3961 gggaaggaag acttggaata agcttggaag gtcagggaga gggtaaattt atcactagag
     4021 gggtagtcca ggacataaaa tgggtaaagg atgatagaaa ggcaaaaata gaatacctga
     4081 atgagaataa tgaagaaatt agactatcac ctggaacaac ctggattcag gtggtaaagc
     4141 cgggaacacc aattactata gaataagaga ggttttttca tgaataggaa agatttgata
     4201 aatagagcaa taaaagcaaa aaagatggtc tacgtaccat actcaggatt tcatgtaggt
     4261 gctgccatac tcactgagga tgatgaaata tacgatggat gcaatataga gattgcttcc
     4321 tattcaccaa ccatatgtgc tgaaagaact gcaatcttca aggcagtctc tgaggggcac
     4381 atgaagatca aggcaattgc cgtggttgga gatgctgacc atacctatcc atgtggggtt
     4441 tgcagacagg tgatccgtga atttggaaag gatgcaaccg tgattattgc aaactcagaa
     4501 gatgattaca gggagtatgc cttggaccag ctgctacctc atagcttcgg acctgaagac
     4561 cttgagtcac cccgtaagga ggagaaaaat gtataaatcc ggattcataa cagtggtagg
     4621 aaggccaaat gttgggaaat cgacacttct caaccaggtg atcggtgaga agatttcaat
     4681 aatctccgac aagcctcaga ccacaagaaa caagatacag atggtctata ccggggatga
     4741 ttttcaaata gtctttttgg atacccctgg aattcagatg ccaaaaaata tactgggaga
     4801 ctacatgctt aagatttcaa gaacaacact cgaagaggtt gacattgtaa
//
LOCUS       DASRQF010000094         3954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_521368, whole genome shotgun sequence.
ACCESSION   DASRQF010000094 DASRQF010000000
VERSION     DASRQF010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521368"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1020)
                     /locus_tag="VFD79_02360"
     CDS             complement(115..1020)
                     /locus_tag="VFD79_02360"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZH92921.1"
                     /translation="MEGGTLLTLQQLKYFLEMCETLHYTKAAKNLNISQPSLSYALNQ
                     LSDELGVPLFAKDGKTIYLTEYGEVFQTYVESAIQILSQGEQQLRNMANPNGGNISLG
                     YIYSVSFDAIPKLIDEFYVNQGDRSTNFSFQVNMTNLLIESLAQGNLDAVLAPLPESV
                     PDFIHSVPILEQELFLLVYNGHPLSQREYVTVEDFQDEKFVMINKGTNLFMQTDALFR
                     KHNIMPEIVFKVDECNSMAAFVGAKLGVAIMPKIPSLDSYRVIAIPFKDREMFRTINL
                     LWNERIEPSSALKTFVEYFSMTRKA"
     gene            1238..2149
                     /locus_tag="VFD79_02365"
     CDS             1238..2149
                     /locus_tag="VFD79_02365"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HZH92922.1"
                     /translation="MNLPSKITICEVGLRDGMQNEPRLFTTEEKVSIANRLIDAGFKV
                     IELGSFMSPKAVPQMANTDEVYRALGKVDGVELRALVANKKGVERAIECGCKKVKLNV
                     SASKAHNLANINMTPEESVSKFKESVDLALMNNLEISGSISMPFGSPWEGEIKLEEVE
                     AIVEAYLSLGIEEISLSDASGMAIPNQVYSICKSMREKYPQVKWWLHFHNTRGLAVAN
                     ILAAMQAGITNFDSSLAGLGGCPFVPGAAGNVSTEDVVHMLDEMGIETGINLERTIAI
                     GKEIQDKVEGGTDSYILKAGRSKDLIR"
     gene            2178..3650
                     /locus_tag="VFD79_02370"
     CDS             2178..3650
                     /locus_tag="VFD79_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZH92923.1"
                     /translation="MSRMSSMDEVYAKLDEYQIAEIEEKFERADKAQKEFEKWSQEEI
                     DRTIQAIAWKVANTKTFKELVKMSIEESKLGDPVSRENKRFKIRGVLRDALRQKSVGV
                     IEELPEKGVVKYAKPVGVIACVIPATNPDLTPAGNAIYALKARNAIIFSPHPRALKTG
                     GKTIELMREGLREVGAPEDLIQILGQGKAKVNKSLSEALNTKCDLVIATGGQGVVRRA
                     YHSGTPAYGVGAGNATMVWDETAKEDLHTAAHNTMLSKTSDFGSGCSADGNIVIHESI
                     YDEAVNELVNVGGYLLNEDEQEAVKKVMWDEECHRLADSVAQSPQKMAELAGFSIGED
                     RKFLIAKGTGSFSKSYEDVWCREKLTTLLAVHKYEGEFVNAIDIVQAIHNVGGKGHSV
                     GIFSFNEDNIDKLALNAQVGRIMVRQPQSKSNAGSFTNGMPMTSSIGCGTWGGNATSE
                     NVNLKHYMNVTWVSRAIPEDRPSDEELFGEFYDPEMEERF"
     gene            3665..>3954
                     /locus_tag="VFD79_02375"
     CDS             3665..>3954
                     /locus_tag="VFD79_02375"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HZH92924.1"
                     /translation="MKRLLSDQIVEYLERREVEYIFGLCGHTVIGLLDALDRNDRVKY
                     ISFRNEQLAAMAADGYARVKKKAGVVMTHLGPGIMNAITGVANASFDSIPMVV"
BASE COUNT         1292 a          664 c          943 g         1055 t
ORIGIN      
        1 tatccagaag ggacattcaa ggaatagaaa attaagaata taatttattg ttatattaca
       61 ggataagaag gcaccctctt aaaacgattc aagagggtgc cttcttatcc tgtgtcaagc
      121 ttttctagtc attgaaaagt attcaacaaa agttttaagt gctgagctag gctcgattct
      181 ctcgttccat aataaattga tggttctaaa catctcccta tccttgaaag ggatggcaat
      241 cactctgtag ctatcaaggg atggtatttt tggcattatt gcaacaccta gctttgcacc
      301 cacaaaagca gccatagaat tgcactcgtc aactttaaag acaatttcgg gcatgatatt
      361 gtgttttcta aatagggcat ctgtctgcat aaaaagattc gtacccttgt tgatcataac
      421 aaatttttca tcctggaagt cttcaaccgt aacatattct ctttgtgaca gtggatgacc
      481 attgtataca aggagaaaca gttcctgctc caatattggg acagaatgta tgaaatcagg
      541 tactgactca ggtaaaggtg caagcacggc atctagatta ccttgtgcaa ggctttcaat
      601 cagtaggttt gtcatgttta cctgaaagct gaaattggtg ctacgatctc cctggttaac
      661 atagaattca tctataagtt ttgggattgc atcaaagcta acgctgtaga tgtacccaag
      721 gcttatgttg ccgccatttg gatttgccat attcctaagc tgttgctctc cttgagaaag
      781 gatctgaatg gcgctttcga cataagtttg gaagacttct ccatactctg taagatatat
      841 ggtctttcca tccttggcaa acaatggaac accaagctca tctgaaagtt gattaagagc
      901 atagctaaga cttggctgtg aaatatttag gttctttgct gctttcgtgt aatgtagtgt
      961 ttcgcacatt tctaagaaat atttaagctg ttgcagtgtc aataatgtac ccccttccaa
     1021 ataataccat agaaaaaagc taaaacccga aactaagaac tgttataaga ttaacgatga
     1081 aatacaagca taaaaaggat tcaacaataa tcattggatg aataatcata gaatgtatct
     1141 atcgttatta tataaaatcg gtattagact ttcaacctat tctatcttaa acttgagata
     1201 agagaattat cagataatta ggaaaggagt gaactatgtg aaccttccat caaaaataac
     1261 aatatgtgaa gtagggctga gagacggaat gcaaaatgaa cccaggcttt tcactacaga
     1321 ggaaaaggtc agtatagcaa atcgattgat agatgctggt ttcaaggtta tagagcttgg
     1381 atcatttatg agcccgaagg ctgttcctca aatggcaaac actgatgaag tttacagagc
     1441 tcttggaaaa gttgacggtg ttgaacttag ggcactggtt gccaataaaa aaggtgttga
     1501 aagagcaatt gaatgtggct gtaagaaagt aaagcttaat gtatcggcaa gtaaggctca
     1561 caatctggca aacattaaca tgacccctga agaaagcgtt tccaaattca aggaaagtgt
     1621 agacttggcg cttatgaaca acctagaaat atctggatcg atttcaatgc cctttggttc
     1681 gccatgggag ggagagatca agctggaaga ggtagaagcg attgtagagg catatttaag
     1741 tcttgggatt gaagagattt ctctttcaga tgcatcgggt atggctattc caaatcaagt
     1801 atactcaata tgcaaaagca tgagggaaaa gtatcctcag gtgaaatggt ggcttcattt
     1861 tcataataca aggggattgg ctgttgcaaa tatccttgct gcaatgcagg cgggaataac
     1921 taactttgat tcatcgttgg ctggattggg aggatgtccc tttgtacctg gtgcggctgg
     1981 gaatgtatca actgaagatg tggtccatat gctggatgaa atgggtattg agaccggaat
     2041 aaacttggag aggaccattg ccataggcaa ggaaattcag gacaaagtag agggaggaac
     2101 agactcctat atattgaagg caggaaggtc taaggacttg attagataga aagactaaaa
     2161 atcaggaggt aatttatatg agcagaatga gttcgatgga tgaggtttat gcaaaactgg
     2221 atgagtatca gatagctgaa attgaagaga agtttgaaag agcggacaag gcgcagaagg
     2281 agtttgagaa gtggagccag gaggaaattg atagaacaat tcaagcaata gcatggaagg
     2341 ttgccaatac aaaaaccttc aaggaacttg tgaaaatgag catcgaggaa agcaagcttg
     2401 gcgatcctgt aagtcgggaa aacaagagat tcaagattcg aggtgtactt agagatgctt
     2461 tgagacaaaa gagtgttgga gtaattgaag agttgcctga aaagggagtt gtaaaatatg
     2521 caaaacctgt tggggtaatt gcatgtgtga taccagctac aaatcctgac ttgacaccgg
     2581 caggcaatgc catatatgca cttaaggcaa gaaatgctat aattttctca ccccatccga
     2641 gagctttgaa gacaggcgga aagacaattg aacttatgcg agaaggcctt agggaagttg
     2701 gtgcacctga ggatttgatc cagatacttg gacagggaaa agccaaggtt aataaatctc
     2761 tttcagaggc attgaataca aagtgcgatc ttgtaatcgc cactggtgga caaggtgtgg
     2821 taagaagagc ataccattct ggaacaccag cctatggagt tggagctgga aatgctacaa
     2881 tggtatggga tgaaactgct aaggaagacc tccatacagc agctcacaat accatgttgt
     2941 caaaaacttc agactttggc tcaggctgtt cagcagatgg gaatatagtc atccatgaaa
     3001 gcatatatga tgaagcagtc aatgaactgg taaatgttgg tggatatctt ctcaatgagg
     3061 atgaacaaga agcagttaag aaggttatgt gggatgagga atgtcacaga cttgcagatt
     3121 cggttgccca gtctccacag aagatggctg agctggctgg tttttcaatt ggtgaagatc
     3181 gaaaattcct tattgcaaaa ggaacaggtt cattctcaaa gtcatacgag gatgtatggt
     3241 gcagagaaaa actgaccact ctactcgcgg ttcataaata cgaaggtgaa tttgtgaatg
     3301 ccatcgatat agtacaggcc attcacaacg ttggaggaaa aggacatagc gttgggatat
     3361 tctcattcaa tgaggataat attgacaaac ttgcccttaa tgcccaggta ggaagaatca
     3421 tggtaagaca gcctcaatcg aaatcaaatg caggctcctt cacaaatgga atgccaatga
     3481 cctcaagcat aggttgtggt acatggggag gaaatgcaac atctgagaat gttaatctta
     3541 agcattacat gaatgttact tgggtttcaa gagccattcc tgaggaccga ccttcagacg
     3601 aagaactgtt tggagaattt tatgatcccg aaatggaaga gagattctag aaggaggaaa
     3661 tacattgaaa agactattat cagatcaaat tgtagaatat ctagaaagac gcgaggtgga
     3721 atatattttt gggctttgcg gacatactgt aatcggtttg ctggatgcac tggacagaaa
     3781 tgacagggtg aagtacatat ccttcaggaa tgagcagctt gcagcaatgg cagcggatgg
     3841 atatgcaaga gtcaagaaaa aagccggtgt tgtcatgact cacctgggtc ctgggataat
     3901 gaatgcaatt acgggtgtgg caaacgcctc ctttgattca ataccaatgg ttgt
//
LOCUS       DASRQF010000095         2184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_148977, whole genome shotgun sequence.
ACCESSION   DASRQF010000095 DASRQF010000000
VERSION     DASRQF010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148977"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1391)
                     /locus_tag="VFD79_02380"
     CDS             complement(144..1391)
                     /locus_tag="VFD79_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HZH92925.1"
                     /translation="MAVLKPFKAHRPAPDLADKVAALPYDVMNSQEAREVVKGNPYSF
                     LHVDKSEVDLPEDIDIHDVKVYEKARDNLYQMIKDGVFIQDKEDSFYIYKQVMKGRAQ
                     VGLVGTTSIDDYLNDIIKKHEFTRADKEEDRINHVDYTNANTGPIFLTYRKKKEISDL
                     INGWMNEKDPIYDFQSDDEITHTIWKIDDKSVIDKLVALFASVDYLYIADGHHRTASA
                     VKVGLKRRDQLPDYDGTEEFNFFLSVLFPDEDLYIMDYNRVVRDLNEYSAAEFIELLK
                     EKFDVEQYTGEGQYKPTEKHSFGMFLDDNWYILRAKEGSFDEDSPVDRLDVSILQKNV
                     LTPLLGIVDPRVDSRIDFVGGIRGLAELEERVINGMRIAFSMYPTSIDDLMAIADAGE
                     VMPPKSTWFEPKLRSGLFVHTLD"
     gene            complement(1404..>2184)
                     /locus_tag="VFD79_02385"
     CDS             complement(1404..>2184)
                     /locus_tag="VFD79_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HZH92926.1"
                     /translation="YDVVVVRSATKIREPIIDAAAGGRLKMIIRAGVGVDNIDVKYAE
                     EKGIKVRNTPNSSSASVAELVLAHMLSLARFVGISNVTMRNGEWNKKKYKGIELAGKT
                     LGLVGFGRISHELAEKASALGMDVIYTNKSGPKDEYPQFSYMELDELLKKSDFVSIHT
                     PAMKDGKPLIDVEKIGLMKDGAYLINCARGGVVDEAALLEALNSGKLSGAGIDVFEVE
                     PAKNTELLNHPNVSVTPHIGGSTHEAQMRIGEEVVEVIKSL"
BASE COUNT          550 a          532 c          410 g          692 t
ORIGIN      
        1 atattgttac tagcagtagt atcattataa ttgcggttga tcttttcaaa aatttcacct
       61 catatccttt atacattttt gtgacgatta tgtcaatgga tttgggcatg gataaggcct
      121 ggctttaaaa ccaggcctaa aacttaatcc agtgtatgga cgaaaagtcc acttcttagc
      181 ttaggctcaa accatgtgga tttaggtggc attacctctc cggcatccgc aatggccatc
      241 aggtcatcga tgctggttgg gtacatggag aaagcaatcc tcatgccatt tataaccctt
      301 tcttcaagtt ctgccaatcc tctgattcca ccaacaaaat caattcttga atcaactcta
      361 gggtccacta ttcccagtag gggggtcaga acgtttttct gtaagattga tacatcaagc
      421 ctgtccacag gtgagtcctc gtcaaaggag ccttccttgg cccttagtat ataccaattg
      481 tcatccagga acattccaaa actatgcttt tctgtgggtt tgtactgacc ttctccagtg
      541 tattgttcaa catcgaactt ttccttcaga agctctatga actccgcagc tgagtattcg
      601 ttaagatctc ttacaacacg gttgtagtcc ataatatata ggtcctcatc cgggaataga
      661 acggatagga aaaagttgaa ctcctcagtt ccatcgtaat cgggtaattg gtctctacgc
      721 tttaggccaa ccttgactgc tgaggctgtc ctgtggtgac cgtcggcaat atagaggtag
      781 tccactgatg cgaacaaggc tacaagcttg tctatgacag acttgtcatc aatcttccat
      841 atggtatgtg taatctcatc atctgattgg aagtcataaa ttggatcctt ttcattcatc
      901 caaccattga ttaggtcgct tatctccttt ttcttcctat aggtaaggaa aatgggacca
      961 gtgttggcat ttgtataatc aacgtggttg atcctatcct cttccttgtc tgcccttgta
     1021 aactcatgtt ttttgattat atcgttcaaa tagtcgtcta tggaggtagt tcccacaagt
     1081 cctacctgtg ctctgccctt cattacttgt ttgtagatgt aaaaactatc ctccttgtcc
     1141 tggataaaga ctccatcctt gatcatctga tagagattgt ctcttgcctt ttcatagacc
     1201 tttacatcgt gtatatcaat atcctccgga agatcaacct cggacttgtc aacatggagg
     1261 aaggaatagg ggttgccctt aaccacctct cttgcctcct ggctgttcat aacgtcatag
     1321 ggtagagctg ctaccttgtc agccaggtct ggtgctggtc tgtgtgcctt gaatggtttc
     1381 aatacggcca tttacatacc tccttaaaga cttttaatca cttcaactac ttcctcgccg
     1441 attctcatct gcgcctcatg ggtagatcct ccaatatgtg gtgttacaga aacattgggg
     1501 tgattcaaca gttcagtatt tttggcaggc tccacttcaa atacatcaat tcctgctcct
     1561 gaaagctttc ctgaattaag tgcctctaga agggctgcct cgtcaacaac tccacccctt
     1621 gcacaattaa tcagatatgc tccatctttc ataagaccta ttttctcaac atctatcaag
     1681 ggctttccgt ccttcatggc aggagtatga attgaaacga agtctgattt ttttagaagc
     1741 tcatccagct ccatgtaaga aaattgagga tactcatcct ttggtccgct cttgttggtg
     1801 tagataacat ccataccaag agcgctggcc ttttcagcca actcgtgaga tatccttcca
     1861 aacccaacca gtcccagggt tttccctgcc agctcgattc ccttgtattt cttcttgttc
     1921 cattcaccat tacgcatggt tacatttgaa attcctacaa agcgtgccag tgaaagcatg
     1981 tgggcaagta caagttcagc aacagatgca ctgcttgaat tgggcgtgtt tctaaccttg
     2041 attcccttct cttcagcata tttgacatcg atgttgtcaa ctccaacacc tgcccttata
     2101 atcatcttaa gccgtccacc agctgcagca tctattatag gctccctaat ctttgtggca
     2161 gacctcacaa ctacaacatc ataa
//
LOCUS       DASRQF010000096         2751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1763804, whole genome shotgun sequence.
ACCESSION   DASRQF010000096 DASRQF010000000
VERSION     DASRQF010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763804"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VFD79_02390"
     CDS             <1..906
                     /locus_tag="VFD79_02390"
                     /inference="COORDINATES: protein motif:HMM:NF044952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HZH92927.1"
                     /translation="DRYIIAEEILKIAEAEGTDTVAHGSTAVGNDQVRFEASLFTLNP
                     NGNIIAPIKELNITRAKEIEYLEERGFKVDKELKKYSINENIFGITVSGTEIDQNKEP
                     DEFAYTLTKRDPSKTEEYLKLEFEKGLPVKLNGKEIQGVDLISKLNAILGSYGYGSRI
                     YVGDCIIGIKGRIMFESPALLALIEAHKKLEQIVLTKRQIMFGKQASTQWSDLVYGGL
                     YYEPLVKNIEALADSVQERVNGTVWIKVQPNSIQVVEMSSPNSLVNANIAMYAQESKW
                     SGTEANGFIKLYSMQQRLANIDWEE"
     gene            910..1722
                     /locus_tag="VFD79_02395"
     CDS             910..1722
                     /locus_tag="VFD79_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92928.1"
                     /translation="MNELLKKYENLNLEVFIVVNRLHWDKYKALMEDFPFKTVFIDPS
                     TTEGYDFTYEMVQLNEKAYGAGMAAPDWTFANFGTIGAGLTAGFLIDGIPVAKLCVVG
                     NVSDPKIAHEWTLLVDPAHEGEGLGSLTFALALHLVQDKDYLTFIIQTDNASSNIYLK
                     NVNPLNILSYGFVHTRKNSMLVKTKIPKENTFETVLGNTTEKFELEDYPMAKDDLESL
                     GDTFWIQQNNSKVYRMINDEIKNGKKYIIKGKHQDNGISYFLISEITPDQEV"
     gene            1744..2706
                     /locus_tag="VFD79_02400"
     CDS             1744..2706
                     /locus_tag="VFD79_02400"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZH92929.1"
                     /translation="MKFLSDLIGINTYEGNYRKMIDYLVKWLKAMDGCQVHTQEIGEN
                     KANVVGIFGEPDLLINCHMDTVKPSRAWSQDPLHMYQKDGRIYGLGACDTKGNIYMVI
                     KALEEKMPKNLMVLFSVDEESGIRTGVKYFLESQYAEGIKRAIVCEPTELKLVNRHRG
                     YYSFTLEDIAESAHSSTGAKGAIIKAAHNVIKLDELGFNVGIIQGGSAGNVVSSSCTY
                     RASIRTFDGLELVVEKIKDNCPGTRIENRFNGPPLINEDPKFEGQFHQVDFWTEGSLF
                     QEAGINTVVFGAGSIDQAHSEDEFVEGWQLEKGIGIIKDIIDKK"
BASE COUNT          848 a          558 c          711 g          634 t
ORIGIN      
        1 gacagatata tcattgcaga ggagatctta aagatagcgg aggcggaggg aacagacaca
       61 gtggcccacg gttccacagc cgttggaaat gaccaggtta ggtttgaggc atcccttttc
      121 acccttaatc caaacggtaa tatcattgcg ccaataaagg aactgaacat cactagggcc
      181 aaggagatag agtatttgga ggaaagaggc ttcaaggttg acaaggagct taagaagtac
      241 tccatcaacg agaatatctt tggaataaca gtttcaggaa ctgaaatcga tcagaacaag
      301 gagccagacg agtttgccta cacactgaca aagagggacc cttcaaagac agaggagtac
      361 ctgaagctgg aatttgaaaa gggacttcca gttaagctta acggtaagga gattcagggc
      421 gtggatctta tatcaaagct taatgcaatc cttggatctt atgggtatgg aagcagaatt
      481 tatgttggag actgcataat cggcataaag ggacggataa tgtttgagtc tcctgccctt
      541 ctggccctaa ttgaggccca caagaagctg gagcagattg tactgaccaa aagacagata
      601 atgtttggca agcaggcatc tacacagtgg tcagaccttg tgtatggggg actctactat
      661 gaacctctgg ttaagaatat agaggccttg gcggattcag tgcaggaaag ggttaacggg
      721 actgtttgga tcaaggttca accaaacagc atccaggttg ttgagatgag ctcacctaac
      781 tccctggtga atgcaaacat cgccatgtat gcacaggaat ccaaatggag cggtactgag
      841 gcaaacggat ttatcaagct ttactcgatg cagcaaagac ttgcaaatat agattgggag
      901 gaataagaaa tgaatgaact attgaaaaag tatgagaacc ttaaccttga ggtatttata
      961 gttgtaaaca gacttcactg ggacaagtac aaggcactta tggaggactt ccccttcaag
     1021 accgtcttca ttgacccatc aacaacggaa ggctacgact tcacctatga gatggtgcag
     1081 cttaatgaga aggcgtacgg ggcgggaatg gctgcaccgg actggacctt cgcaaacttc
     1141 ggaaccatcg gggcaggcct gactgcagga tttttgattg atggaatacc cgttgccaag
     1201 ctttgtgtgg ttggaaatgt ttcagacccc aagatagccc acgagtggac acttcttgtg
     1261 gatccagccc atgagggaga gggacttggg tcacttacct ttgcacttgc attgcatctt
     1321 gttcaggaca aggattatct gaccttcata attcagaccg acaatgcaag ctcaaatatc
     1381 tatcttaaga atgtgaatcc ccttaacatc ctgtcctacg gctttgttca cacccgtaag
     1441 aacagcatgc tggtaaagac caagatacct aaggagaaca cctttgaaac agtactaggg
     1501 aatacaactg agaagtttga gcttgaagac tatccaatgg caaaggacga tttggaaagt
     1561 cttggggata ccttctggat tcagcaaaac aattccaagg tatacagaat gatcaacgac
     1621 gagatcaaga acggcaagaa gtatattatc aagggcaagc atcaggataa cggcatatcg
     1681 tacttcctga tttctgaaat aaccccagac caggaagtct agaagaggaa agtgggtagt
     1741 tggatgaaat tcttgtcaga cctaatagga ataaacacat atgagggcaa ttacagaaag
     1801 atgattgact atctggtaaa atggctgaag gctatggacg gctgccaggt tcatacccag
     1861 gagatcggag agaacaaggc caatgtggta ggaatctttg gagagccgga cctcctgatc
     1921 aactgccaca tggatactgt caagccctcc agggcctggt cccaggaccc tcttcatatg
     1981 taccaaaagg atggaaggat ctacggtctt ggggcctgtg acacaaaggg aaacatctac
     2041 atggtcataa aggctctgga ggagaagatg cccaaaaacc ttatggtcct cttttcagtt
     2101 gatgaggagt ctggaataag gaccggggtt aagtacttcc tggaatccca atatgcggag
     2161 ggaatcaaga gggccatagt ctgcgagcca acagagctta agctggtcaa caggcacagg
     2221 ggctactact cctttactct ggaggatatt gcagaatcag cccattcctc aacaggggcc
     2281 aagggagcaa tcataaaggc agcccacaat gtgatcaaac ttgatgagct gggctttaat
     2341 gtgggcataa tccagggtgg atcagcggga aatgttgtat cctcaagctg cacctacagg
     2401 gcatccataa ggacctttga tggcctggaa cttgttgttg aaaaaatcaa ggacaattgc
     2461 ccaggtacaa gaattgaaaa tcgcttcaac gggcctcccc tgataaatga agaccccaag
     2521 tttgagggcc aattccacca ggttgacttc tggaccgagg gcagcctttt ccaggaggct
     2581 ggaatcaata cggtggtatt cggggcagga agcatagacc aggcccattc ggaggatgag
     2641 tttgttgaag ggtggcagct tgaaaaggga atcgggataa taaaggacat tattgataaa
     2701 aaataagaat attgtacatt tagaaccata tggcctggct gtatggtttt t
//
LOCUS       DASRQF010000097         3957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_274858, whole genome shotgun sequence.
ACCESSION   DASRQF010000097 DASRQF010000000
VERSION     DASRQF010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274858"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..133
                     /gene="rrf"
                     /locus_tag="VFD79_02405"
     rRNA            17..133
                     /gene="rrf"
                     /locus_tag="VFD79_02405"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            329..1942
                     /locus_tag="VFD79_02410"
     CDS             329..1942
                     /locus_tag="VFD79_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZH92930.1"
                     /translation="MNRKGKDLLEIEDENDAGKIITEILSELGIQENINDGEVARNLM
                     KYRGKELLLYTGLFETKGFKGVFVEFNPLSKHKEELKAFDYEVQASSLLRTVLDSSNE
                     SFIYVNSEGIIEMISESYAEFLNRKKEELIGLNVVDVVDNTRLDVVLKTGKAEIAQIQ
                     IVKGKKIIATRIPVYAKGKVVGAIGKILFRDVDELNNLYMKINNIEKELNLYRDEFKK
                     ANKAEYALDSIIGENIEIKRIKDLTRSIASTNSNVLILGESGTGKELFAHAIHNNSKR
                     MDAPFIKVNCGAIPYELLESELFGYEEGAFTGAKKGGKIGKFKAADGGTIFLDEIADL
                     PMNMQVKLLRVLQEREIERIGSNTSEKVDLRIIAATNKDLERMVEEETFRLDLYYRLN
                     VVSLRIPSLNQRKDDIQILAEHLIEKISIKENRYVDGISPKSLEMLKAYSWPGNIREL
                     ENVLERAINFLGEERTILPKHLPPKIAGSQDLYVNRSLRETLEEVEKEAIVRTLFHVK
                     GNKSKASKALGISRTSLYEKMDKYRIENS"
     gene            2259..3437
                     /locus_tag="VFD79_02415"
     CDS             2259..3437
                     /locus_tag="VFD79_02415"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008900891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HZH92931.1"
                     /translation="MKKAVIVSAVRTPVGSYGGVFKNTSAVELGKVAVKAAIERAGVK
                     PEMIDEVIFGNVLQAGQGQNVARQVAIGAGIPDHVTSYTVNKVCGSGLKTVALAAQAI
                     KAGDAEVVVVGGTENMSMAPYLLPNNRWGQRMGNGQVIDYMVADGLTDIFNNYHMGIT
                     AENIVEKYGLTREEQDEIAYNSQTRAERAIKEGRFKEEIVPVEIPQRKGDPIVVDTDE
                     YPKFGTTMETLAKLRPAFKKDGSVTAGNASGINDGAAAFVVMSEDKAKELGLKVMAEI
                     VSYASGGVDPSIMGTGPIPASKNALDKAGLKVEDMDLIEANEAFAAQALSVAKELNFD
                     MDKVNVNGGAIAIGHPIGASGARILVTLLHEMEKRDNKLGLATLCIGGGQGISMVVKR
                     "
     gene            3466..>3957
                     /locus_tag="VFD79_02420"
     CDS             3466..>3957
                     /locus_tag="VFD79_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HZH92932.1"
                     /translation="MNKVITIDEAIDKIQDGMTIMVGGFLGCGNPHRLVDKLVEKGTK
                     DLTLICNDSSFPDYGVGKLIVNKQIKKLIASHVGTNPETGRQMNEGEMEVVLVPQGTL
                     AEQVRAGGSGLGGFLTPTGVGTVVEEGKQKIELDGVEYLLELPLKADVALIAGQTADK
                     FGNI"
BASE COUNT         1344 a          614 c         1001 g          998 t
ORIGIN      
        1 aagtttgtag ttttcgtctg gtgacgatag caagagggag acacctgtac ccataccgaa
       61 cacagaagtt aagcctctta acgccgatgg tacttggttg gtaacgacct gggagagtag
      121 gaagtcgcca gtctatatat agcaaaggct ctgttgatta ttcaacaggg tctttttgtg
      181 ttcaaaaaat tgacactaat ggtataatga agataaatac gagttagtga ggtgaaaagt
      241 tataaatacc aatatttcag caagggatat aataaatata tacgactcag gatttattat
      301 tctggatgag gactacaagc tacttttagt gaacaggaag ggaaaggacc tgctggagat
      361 agaagacgaa aatgatgctg ggaagataat tacagagatc ctttcagaac ttgggattca
      421 ggaaaacatc aatgacggag aggtagctag aaaccttatg aagtataggg gaaaggagct
      481 tctgttgtac acgggcttgt ttgaaaccaa aggatttaaa ggggtgtttg ttgaattcaa
      541 tcctttaagt aagcacaagg aagaattgaa ggcctttgat tatgaggtac aggcatcgtc
      601 tctcctaagg acagttctgg attcatcaaa tgagtccttc atttatgtaa acagcgaagg
      661 tatcattgaa atgataagtg aaagctatgc cgaatttctc aataggaaaa aggaggaact
      721 tatcggactt aatgtggtgg atgttgttga taataccagg cttgatgttg tacttaagac
      781 aggcaaggca gaaatagccc agattcagat tgtaaagggc aagaagataa ttgccaccag
      841 gattcctgtt tatgcaaagg gaaaagtggt tggagcaatt ggaaagatac tgtttagaga
      901 tgttgatgaa ctaaataatc tgtatatgaa gatcaacaat atcgaaaagg aactaaacct
      961 gtatagagac gagttcaaga aggcaaacaa ggcagaatat gcccttgata gcataatagg
     1021 tgaaaacatt gaaataaaga ggataaaaga ccttacaaga agcatcgcat ctacgaactc
     1081 caacgtctta atcctggggg aatcaggtac cggcaaggaa ttgtttgccc acgccataca
     1141 caacaacagt aaaaggatgg atgccccctt tataaaggtt aattgtggtg caatacccta
     1201 tgaactactt gaatcagaac tttttggcta tgaggaaggt gccttcactg gtgccaagaa
     1261 gggtgggaaa ataggtaagt tcaaggctgc agatggtggg acaatttttt tggatgaaat
     1321 agcagacctc cccatgaata tgcaggtgaa gcttctcagg gtccttcagg agagggagat
     1381 tgagagaatc ggctccaata cttccgaaaa ggttgattta aggattatcg ctgcaaccaa
     1441 taaggacctt gaaagaatgg tggaggagga aacattcagg ctggatcttt actacaggct
     1501 aaatgtggtt agtttaagaa taccttcgct aaatcagaga aaggatgaca tccagattct
     1561 cgctgaacac cttattgaaa aaataagcat taaagaaaat aggtacgttg atggaatatc
     1621 ccccaaatca ctggaaatgc tcaaagctta ctcttggcca ggaaacatca gagagcttga
     1681 gaatgttctt gaaagagcaa taaattttct gggtgaggaa agaacaatac tgccaaagca
     1741 tttgccacct aaaatagcgg ggagtcagga tttatatgta aatagaagct taagggaaac
     1801 tctggaggaa gttgaaaagg aggcaattgt aaggactttg ttccatgtta agggtaataa
     1861 gtcaaaggca tcaaaggctc ttggaataag caggacaagc ctctatgaga aaatggacaa
     1921 gtataggatt gagaatagtt aataatataa caaacaccat tattgttcat ttgttgaaca
     1981 ataatgggaa tgcatatgga attatgtacg ctaaagttta caacacccta taggccttag
     2041 aaagtatgta aggtaaaact tacaataatt tggagtaggt atattatttt tgggtaaatt
     2101 aaccagtaag aaggtcaatt atgtgaataa atgattaaat attaacaaac ttaatcattt
     2161 atatagattt tctgtagttt tatcagatga aataacacat aagttggcat ggtaattgca
     2221 agttattagg ttaagaattc aatgaggagg gttttagaat gaaaaaagct gtaatagtaa
     2281 gtgctgtaag aacacctgtg ggtagctatg gaggagtatt caagaatact agcgccgttg
     2341 agcttggtaa ggttgccgtt aaggctgcga ttgaaagagc gggagttaag ccggaaatga
     2401 ttgacgaggt aatatttgga aacgttcttc aggctggtca gggacagaac gttgcaagac
     2461 aggttgcaat cggagctggg ataccagatc atgtgacatc atatacagtc aacaaggttt
     2521 gcggatcagg acttaagacc gttgcattgg cggctcaggc tatcaaggcc ggagatgccg
     2581 aggtggtagt tgttggtggt acagaaaaca tgagcatggc tccttacctg cttccaaaca
     2641 acagatgggg acagagaatg ggtaatgggc aagtgattga ctatatggtg gcagatggac
     2701 ttactgatat cttcaataat taccacatgg gaattacagc agagaatatt gttgaaaaat
     2761 acggcctgac aagagaagag caggatgaga ttgcatataa ttcacaaact agagcggaaa
     2821 gggcaattaa ggaaggaaga ttcaaggaag aaatcgttcc tgtggagatc cctcaaagaa
     2881 aaggagatcc aatagtagtg gatacagatg agtatccaaa atttggtaca acaatggaaa
     2941 ccctggctaa attgagaccg gcattcaaga aggatggatc agttactgcc ggtaacgctt
     3001 caggtatcaa tgatggggca gcagcctttg tcgttatgag tgaagacaag gctaaagagc
     3061 taggccttaa ggttatggct gaaatagtaa gctatgcttc aggtggagtt gatccatcaa
     3121 tcatgggaac aggaccaata cctgccagca agaatgcatt ggacaaggct ggactaaaag
     3181 ttgaggacat ggatttgatt gaagcaaatg aagcttttgc tgcacaggcc ctttcagttg
     3241 ccaaagaact taactttgat atggacaagg taaatgtcaa tggtggagct attgcaattg
     3301 gacacccaat cggagccagt ggtgcaagaa tacttgtaac tctacttcat gaaatggaga
     3361 aaagagacaa caagctgggt ctggcaacac tttgcatcgg tggtggacaa ggtatttcaa
     3421 tggttgttaa gcgataataa atcataatga aaggcaggta tccatatgaa taaagtaatt
     3481 acaattgatg aagccatcga caagattcag gatggcatga ctataatggt tggaggattc
     3541 cttggttgcg gaaaccctca tagattggta gacaagcttg ttgagaaggg aaccaaggac
     3601 ctaacattga tctgcaatga ctcatcattt ccagattatg gagttggcaa gcttattgta
     3661 aacaagcaga tcaagaagtt gattgcatct cacgtaggta caaaccctga gaccggaaga
     3721 cagatgaatg aaggtgagat ggaggtagtt cttgtaccac agggaacact tgccgagcag
     3781 gtaagagcag gcggatcagg cctaggtgga ttcctgactc caacaggcgt aggcactgtt
     3841 gttgaggaag gaaaacagaa gattgaattg gatggggttg agtaccttct ggaacttcca
     3901 ctaaaggcag atgttgcact gatagcaggg cagactgctg acaagtttgg taatatt
//
LOCUS       DASRQF010000098         8120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_519593, whole genome shotgun sequence.
ACCESSION   DASRQF010000098 DASRQF010000000
VERSION     DASRQF010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 8120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8120
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519593"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..1043
                     /gene="selD"
                     /locus_tag="VFD79_02425"
     CDS             30..1043
                     /gene="selD"
                     /locus_tag="VFD79_02425"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011592930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:48..50,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HZH92933.1"
                     /translation="MTKSSGUAAKIGPDVLAQVLCHLPKTNDSNLLVGIESSDDAAVY
                     RINDDLAIIQTLDFFTPVVDDPYTFGQIAAANSLSDIYAMGGDPKLALNIVCFPDCLD
                     PEILAQIMKGGHDKVTEAGAILVGGHSVSDDEPKYGLSVTGFVHPDKVQANNTAKPGD
                     VLVLTKPLGIGIITTAIKGDLASDDARDKAILSMATLNIHGKEAIDATEEVHSVTDIT
                     GFGLLGHSLEMAEGSGVTIKIFSSRVPYIQEAKEYSQMGLIPEGAYNNRNFIGDKVKI
                     HESVPEYLKDILFDPQTSGGLLISVARESVDNLLEKLSKSPVEFGVVGEVISKDEKQL
                     IVE"
     gene            1058..2482
                     /gene="selA"
                     /locus_tag="VFD79_02430"
     CDS             1058..2482
                     /gene="selA"
                     /locus_tag="VFD79_02430"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HZH92934.1"
                     /translation="MSDVNLFKMIPKVDELLENELIKNLDKKVPRKTMVDSIRLATEN
                     VRTAIKDGREPEEIKVMVRSLADTIVKMAIKKNSFQLRPVINATGVVIHTNLGRSPVS
                     PEILDHIKDVSVGYSNLEYDLEKGSRGSRYSHLEDLVADITGAESAMVVNNNAAAVLL
                     VLSTLASGREVIVSRGELIEIGGAFRIPDVMSQSGAKLVEVGTTNKTHLRDYEGAISE
                     DTGAIMKVHTSNYRIMGFTSAVEASDLKDVSERHRIPLIEDLGSGVLIDLSKYGITYE
                     PTVMDSLKSGVDIVTFSGDKLLGGPQAGIIVGKKEYIDMMKKNPLTRAFRVDKFTISA
                     LEATFRYYLDMDAAPSQIPTLRMLTYKMEVLQEKAEKLMEKLVGDQVQTKVGLSIQDE
                     YSEVGGGSLPMEKLPTKCVVVELGEGSTARFEDLLRTNTIPVIARIHRDKVYLDVRTI
                     DDSEMEIVSKAVRRAVDGLEECIE"
     gene            2479..4389
                     /gene="selB"
                     /locus_tag="VFD79_02435"
     CDS             2479..4389
                     /gene="selB"
                     /locus_tag="VFD79_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HZH92935.1"
                     /translation="MKHLIIGTAGHIDHGKTTLIKALTGRETDRLKEEKDRGISIELG
                     FTYFDLPNGQRAGIIDVPGHEKFIRNMLAGVVGIDIVMLVVAADEGIMPQTAEHLAIL
                     DMAGVKKGFVVITKADMVEEEWIDLVKEEVWEAVEGTFLEGSQIIPVSSTKKTGIDKV
                     ISLIQDMAVELEDKNVNDMPRLPVDRVFTISGFGTVVTGTLLAGKFSIGDEVEIYPSG
                     IKSRIRSIQVHDQDSKEAFGGQRVAMNLANVKKDDIERGNVVAPINAMVTTMMLDVKI
                     RLLKGLDKVIENRTRLKLYLGTEEILCRIVLLDRETLEPGEEAYAQLRLESETVAKRG
                     DRFILRFYSPMFTIGGGEILEPNPAKRKRFDEDDIQELIIKDEGDLKDIVEHMIIDKA
                     KLYLSVKEMARETSMLEENIIQEIRNLQDEGKITIYQLSKDIYPVHLDHITSLVQSME
                     KVLQVFHSKYPLRIGASKEEMRSRFLQKAKPKLAENIIEDLIQKGHFKQEKDLISLQD
                     HKPQYSRDQLEIKERIMDQFRRDPFMPPKKEDLIKSELGKHEDIDEVLMDMLNRQDLV
                     RVSDDLLILPENLERAKNELVGHIQKHGGVSVAEYRDLLNTNRKNAIGLLEYFDQIKI
                     TQRDGEKRLLVN"
     gene            4409..5098
                     /locus_tag="VFD79_02440"
     CDS             4409..5098
                     /locus_tag="VFD79_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZH92936.1"
                     /translation="MSGRILIIDNDRSFVKGLRYSLEQDDYRIDILSEGKDTLDHITR
                     KEYNMVLIETELPDINGLTLCQDIRNISSVPIIFITSVDEDIKKILALEYGADDYLVK
                     PFNILELKARIKAVLRRINHKVSDNDSNVVEIKDLRINSVGRKVTRSDKNISLTGKEF
                     DLLYVLASNPGKVFTREELLEKVWGYAYYGDLRTVDVHIRRIREKIEKDPKAAEYVLT
                     KWGVGYFFKEN"
     gene            5147..5243
                     /locus_tag="VFD79_02445"
     tRNA            5147..5243
                     /locus_tag="VFD79_02445"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5181..5183,aa:Sec,seq:tca)
     gene            complement(5310..6509)
                     /locus_tag="VFD79_02450"
     CDS             complement(5310..6509)
                     /locus_tag="VFD79_02450"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HZH92937.1"
                     /translation="MTRKLYWENPYLHETEASIIKKEKKEGKYHVRLDRTIFYPDMSG
                     GQPGDVGTINGERVIETIEHEDGLTHILESQVTGPKVKLSIQSDHRFDMMQQHTGQHL
                     LSGVLYNLLGGETISFHLGKDYSSIDVTLTDMDHEEASNVEALCNRIIQSNFKVKTYI
                     VNQEKLRLLPVRKSPSVDTNIRMVEIDGFDYSPCGGTHVNHTGELGLLKITKWDHYKG
                     NLRIQFLTGQRAFVDYSSKFKSVRSISNLLSSSDDNIEAKVEKFLMDKINLEKEVRSG
                     REELISARSSVLVEGSEKIGDMSLISKMYDNFDFKALSHLGSHISNNYERVVQIYGIS
                     NDGLGQFIISKSKDISIDLGNVYKQLAGEFKLKGGGNTNTVQGSVPTVNLDSLLQRAR
                     EIITQGL"
     gene            6632..>8120
                     /locus_tag="VFD79_02455"
     CDS             6632..>8120
                     /locus_tag="VFD79_02455"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HZH92938.1"
                     /translation="MKRPALIPALILSLGILTAYYFRISSTTTIIFSMFVLVIMIGSL
                     STGRRVGRIYLMLPFLLAILYTNHFHVSSLEKYEGYPVQIFGVVDREYVREGYTSFDI
                     IVESINGERLGEKVRTNIYQDEEIGRGSRMSLRGQLYRPMENTNPMLYNHRKYLLTKN
                     IKYMVRTEEISHHPNSGPIEFGYGMQNSFKDRLDKVFDKGLSPVNSGFMKALLTGEKD
                     SLEQEEYDLYREMGIAHILAISGLHIGILAGFLLFIMSRSGVKRNVAIPLALVVIWTF
                     TYLVGLPESALRASIMLTFVLLSKIIHRPYDPINILAASYIVCLLINPFWIFSLGFQL
                     SYGATLSLAAIAPWIMERLYPAKGKLARSISAVAGVNIGLLPLQSYYFNQLPLMALFS
                     NLLIVPVATANLILGFMAILIPALTPILDLLLDIQRYMIVLISSLPIEALTVASPGPH
                     QMILYVFIIIVVLKWKDISYFYIRVRKVILLYLLFVSVIGFLGFNE"
BASE COUNT         2420 a         1581 c         1962 g         2157 t
ORIGIN      
        1 gaattaaatt gttggataga ttgacccaaa tgacaaaaag ctctggatga gcggcaaaaa
       61 taggaccgga tgtcctggca caggttctgt gccatttacc aaagaccaat gacagtaatt
      121 tattggttgg aatcgaatca tctgatgatg ctgcagtgta taggataaat gatgatttgg
      181 caataattca gacattggac tttttcaccc cagtggtgga tgatccctac acctttggcc
      241 agattgcagc ggccaactcc ctaagcgaca tatacgctat gggtggggac ccaaagctgg
      301 cccttaatat cgtttgtttc cctgattgcc tggatcctga aatcctggcc caaataatga
      361 agggaggcca tgacaaggtc actgaagccg gtgccatact ggtgggaggc cacagtgttt
      421 ccgatgatga gcctaagtat ggactctcgg tgacaggctt tgtacatcct gacaaggttc
      481 aggcaaacaa cacagcaaaa cctggagatg tgctggtact gaccaagcct ctgggcatag
      541 gaatcatcac aactgcaata aagggagacc ttgccagtga tgatgccaga gacaaggcca
      601 tcctttcgat ggctacattg aacatccacg ggaaggaagc catagatgcc acagaagaag
      661 ttcactccgt aacagatatc acaggctttg gattgttggg gcacagcctt gaaatggctg
      721 aaggtagtgg agtcacaatc aaaatattca gcagcagggt gccctacatc caggaagcca
      781 aggaatactc gcagatgggc ctgattcctg aaggagccta caacaacaga aatttcatag
      841 gagacaaggt caaaatccat gagtcggtac ctgaatacct gaaggatatc ctattcgacc
      901 ctcaaacatc aggggggctt ttgatttcag tcgcaaggga aagtgtggat aaccttttgg
      961 agaagctttc caaatcacca gtggagtttg gagttgttgg agaagtaata tcaaaagatg
     1021 aaaaacagtt gatcgtagaa taaaggattg gtggaaaatg agcgatgtta atctatttaa
     1081 aatgattccc aaggtggatg agctcctgga gaatgagcta ataaaaaacc tggacaaaaa
     1141 ggtaccaaga aagaccatgg ttgattcaat tcgacttgcc acggagaatg tcagaactgc
     1201 cataaaggat ggaagggagc ctgaggaaat taaggtcatg gtcaggagcc tggcagacac
     1261 cattgtcaag atggcgatca agaagaattc atttcaattg agacctgtca taaatgcaac
     1321 aggagtggta attcacacaa accttggaag gtcaccggtt agccctgaaa tacttgacca
     1381 tataaaggat gtatcagtgg gctattcaaa tcttgaatat gacctggaaa agggaagtag
     1441 gggatcacgc tactctcatt tggaggatct tgtggcggac ataacaggtg ctgagtcggc
     1501 catggttgta aacaacaatg ctgcagcggt gcttcttgtt ttaagtactc tggcgtcggg
     1561 aagggaagta attgtatcaa gaggggaact gatcgaaata ggtggggcat tcagaatccc
     1621 ggatgtaatg tctcaaagtg gtgctaagct tgtggaggtt ggaaccacaa acaagaccca
     1681 cctgagggat tatgaaggtg cgatctccga ggacactggt gcaataatga aggtccatac
     1741 ctccaactat agaataatgg ggttcacttc agctgtggag gcctctgacc ttaaggatgt
     1801 ttcagaaagg cacaggatac cacttattga ggatttgggc agcggtgtac tcattgacct
     1861 ttcaaaatat ggaataacat atgagccaac tgtcatggat agcctgaaga gcggggtgga
     1921 tattgttacc tttagcggag acaagctcct gggaggacca caggcaggca taattgtagg
     1981 caagaaggaa tatatcgata tgatgaagaa gaatccgctc acaagggcct tcagggtgga
     2041 caagtttaca atatcagccc ttgaagcaac ctttaggtat tatttggaca tggatgctgc
     2101 cccatcccag attccaaccc tgagaatgtt aacctataaa atggaagttc ttcaggaaaa
     2161 ggctgagaag ctcatggaaa aactggttgg agaccaggtt cagacaaaag tgggcttgtc
     2221 catacaggat gagtattcag aggttggggg aggatcatta cccatggaaa agcttccaac
     2281 aaaatgcgta gtcgttgagc ttggggaagg aagcacagca aggtttgagg accttttaag
     2341 aaccaacacc attccggtca tagcaagaat ccatagggat aaggtctatc ttgatgtgag
     2401 aaccatagat gattctgaaa tggaaatagt gtccaaagcc gtccggaggg cagttgacgg
     2461 actggaggag tgtatagaat gaagcatttg attattggaa cagccggtca catagaccat
     2521 ggaaagacga cccttatcaa ggccttgaca ggaagggaaa ccgataggtt aaaggaggag
     2581 aaggacaggg ggatatccat tgaacttggc ttcacatact tcgatcttcc caacggtcag
     2641 agagcgggta taattgatgt gccaggacat gaaaagttca taaggaatat gctggcaggt
     2701 gttgttggga tagatattgt tatgctggtc gtggcagctg atgagggaat aatgccgcaa
     2761 accgccgagc atcttgccat acttgacatg gctggtgtga agaagggatt tgttgtaata
     2821 acaaaggcag acatggtgga ggaagagtgg atcgatctgg taaaggagga ggtttgggaa
     2881 gcggtagagg gaacttttct cgaaggaagc cagataattc cagtatcatc aacaaagaag
     2941 acaggaatcg acaaggttat cagtttgata caggacatgg ctgtggaact tgaggacaag
     3001 aatgtaaatg acatgcccag acttcctgta gacagggtgt ttacaatttc tggatttgga
     3061 acggttgtta caggtacctt gcttgcagga aaattttcca ttggagacga ggtcgagatt
     3121 tacccttctg gcataaaatc gagaataagg tcaatacaag ttcatgacca ggattccaag
     3181 gaggcttttg ggggtcagag ggtggctatg aatcttgcca atgtaaagaa ggatgacatt
     3241 gaaaggggta atgttgtggc cccaataaat gccatggtca ccaccatgat gcttgatgta
     3301 aagataaggc tccttaaagg tcttgataag gtcattgaaa accgtacgcg acttaagctc
     3361 taccttggga ccgaagagat cctgtgcagg atcgtactcc ttgacaggga aacccttgag
     3421 cctggtgagg aggcatacgc ccaactgagg cttgaatccg aaaccgtagc caaaagagga
     3481 gacaggttta tattgagatt ctattctcca atgttcacaa tcggtggagg ggaaatactt
     3541 gagcctaatc ctgccaagag aaagcgattt gatgaggatg acatccagga actcattata
     3601 aaggatgaag gggacttaaa ggacatagtt gaacatatga tcattgacaa ggccaagctt
     3661 tacttatctg tcaaggaaat ggccagggaa acgtcaatgc tggaggaaaa cataatccag
     3721 gaaataagaa atcttcagga tgaaggcaag ataaccatat accagctgag caaggacata
     3781 tatccagttc atttggacca tatcaccagt ttggtccaat ccatggagaa ggtgctgcaa
     3841 gtgtttcaca gcaaataccc gttgaggatt ggagcttcaa aggaggaaat gagaagcaga
     3901 ttcctacaga aggcaaagcc aaagcttgca gagaatataa ttgaagacct gatccagaaa
     3961 ggacacttca aacaggagaa ggacctgatt tcccttcagg atcacaagcc tcaatattca
     4021 agggaccagc ttgagataaa ggagagaata atggaccaat tccgcaggga cccctttatg
     4081 cctccaaaga aagaggattt gataaagtct gaattgggta aacacgaaga tatagatgag
     4141 gtattaatgg atatgctcaa caggcaggac cttgtcaggg taagtgacga cctcttaata
     4201 ttgcctgaaa atcttgaaag ggcaaagaat gagctagtcg gacacatcca gaagcatggc
     4261 ggagtttcag tggctgagta cagggatttg ttgaatacaa atagaaaaaa cgcaatcggt
     4321 cttcttgaat attttgacca gattaaaatc actcaaagag atggcgagaa gagattactt
     4381 gtaaattgac attgaggtga gggtaggtat gagcggtaga atactgatta tagacaacga
     4441 ccggtcattt gtgaaggggc ttcgttatag ccttgagcag gatgactatc gcatagacat
     4501 actttccgag ggaaaggata ccctggacca cataactagg aaagaataca acatggtcct
     4561 gattgagacc gagctgcctg atattaatgg actgacatta tgccaggata tccgtaacat
     4621 ttcatcagtg ccaataatct ttataacctc ggtggatgag gatatcaaga aaattctcgc
     4681 cctggagtat ggggctgatg actacctggt aaagccattt aacattctgg agttaaaggc
     4741 caggatcaag gccgtacttc gaaggataaa ccacaaggtt tcagacaatg acagtaatgt
     4801 tgttgagatc aaggacctaa ggatcaattc agtgggcaga aaagtgacca gaagcgacaa
     4861 gaatatttcc ctgacaggta aggaatttga ccttttatat gtgcttgcat ccaatccagg
     4921 caaggtgttc acaagggagg aactgctgga gaaggtatgg ggatatgcat attatgggga
     4981 tctaaggact gtggatgtcc atattcgtag aattcgggaa aagattgaaa aagaccccaa
     5041 ggcggcggaa tatgttctaa ccaaatgggg tgttggatat tttttcaagg aaaattagga
     5101 ttgaaatcca ttaggattta ttgtataatg gctcttgtag ccaaatgggg gtagaggagt
     5161 cctggtgggc tctctggtct tcaaaaccag cgcggggcgt taggagcgtc ctgggtgagt
     5221 tcgattctca catactcccg ccaacttgca ccaaagtaaa agcaccttat tagggtgctt
     5281 tttttgtgca agtttttatt gtctcaaggc tagagtccct gtgtgataat ttcccttgcc
     5341 ctttgaagca gtgaatccag gttgactgta ggtacggagc cttgaacagt gtttgtgttt
     5401 ccccctccct ttagcttgaa ctctccagct agttgcttat agacattacc cagatcaatg
     5461 ctgatatctt ttgatttgct tattatgaac tgccccagac catcattgga tattccataa
     5521 atctgtacaa ccctttcata attgttggat atgtggcttc ccagatggct tagagccttg
     5581 aaatcaaagt tgtcatacat cttgcttatc aggctcatat ctccaatctt ttctgaaccc
     5641 tccacaagca cagagcttct ggctgaaatc aactcttccc ttccagacct gacctccttc
     5701 tcaaggttga ttttgtccat taaaaacttt tcaacctttg cttcaatgtt atcatcggaa
     5761 gaggacagga ggtttgatat tgatcgcaca gacttgaact tggaggaata atccacaaat
     5821 gccctttgtc cagtcaaaaa ctgaattctg agattgcctt tatagtggtc ccactttgtt
     5881 attttcaaga gtccaagctc accagtatgg tttacatgtg taccaccaca tggagagtaa
     5941 tcaaaaccgt ctatctcaac catcctaata ttagtatcca ctgagggtga ctttcttaca
     6001 ggtagtagcc tgagtttttc ttgatttaca atgtatgtct ttaccttgaa gtttgattgt
     6061 attatcctgt tgcacagggc ctcaacatta ctcgcttctt catggtccat atccgtcagg
     6121 gttacatcga ttgaggagta gtcctttccc aaatgaaagc ttatggtttc tcctcccaaa
     6181 agattgtaaa ggacacctga tagaaggtgt tggcctgtat gctgctgcat catgtcgaat
     6241 ctgtggtccg attggatgga taacttgacc ttaggccctg ttacctggga ttcgagtatg
     6301 tgagtcaggc catcttcatg ctcgatggtc tcaattaccc tttccccgtt tatggtccca
     6361 acatccccag gttgaccccc tgacatatca ggatagaata ttgtcctgtc cagtcttacg
     6421 tggtattttc cttccttctt ttccttcttg ataatggaag cctcagtttc atgcaaataa
     6481 ggattctccc aatagagctt tcttgtcata gaagcaccgc ctttaaattt gtaattttaa
     6541 atatttgaaa agaaataatc catttatatg ttataattag cttaattata taacaaggaa
     6601 ccataaatat aaagtgctga ggagataata catgaagcga cctgcattga ttcctgccct
     6661 tatcttaagc ctgggaattc ttacagctta ctatttcagg atttcgtcaa ctacaacgat
     6721 tatattctca atgtttgtgc ttgtcatcat gataggatcc ttatccactg gcagaagggt
     6781 gggaaggata tatcttatgc tccctttcct tctagcaatc ctatacacca atcattttca
     6841 tgtatcttcc ttggaaaagt atgaaggtta ccctgttcaa atatttggag ttgtggacag
     6901 ggagtacgtc agggagggct acacctcctt tgatataatc gttgagagca taaatggaga
     6961 gagacttggg gaaaaggtaa gaacaaacat ataccaggat gaggaaatag gccgtggatc
     7021 aaggatgtca cttaggggcc aattgtaccg acccatggaa aacaccaacc caatgcttta
     7081 caaccataga aagtacctat tgaccaagaa cataaaatac atggtgcgta cagaggaaat
     7141 ttcacaccat cctaattccg gacccataga gtttggatat gggatgcaaa attccttcaa
     7201 ggataggttg gataaggtct ttgacaaggg tctttcacca gttaattcgg gttttatgaa
     7261 ggccctgctg acaggtgaaa aggactcatt ggagcaggaa gagtatgacc tgtacagaga
     7321 aatgggcatt gcacacattt tggctatctc aggacttcac ataggtatcc tggcaggatt
     7381 cctactcttc attatgtcaa ggtcaggagt gaagagaaat gtggcgattc cactggcact
     7441 ggtggtaatt tggacattta catatcttgt cggacttccc gaatcagccc ttagggcttc
     7501 aatcatgctt acatttgttc tattgtccaa aataattcac aggccatatg acccgattaa
     7561 tattttggcg gcatcatata ttgtatgcct cctgatcaat cccttctgga ttttcagctt
     7621 gggatttcaa ttgtcctatg gcgcaacact atccctggct gcaatcgcac cctggatcat
     7681 ggagcggcta tatcctgcaa aaggaaagct tgcaaggtcc ataagtgctg tagcaggagt
     7741 aaatattggc ttactgcccc tgcaaagcta ttatttcaat caattgcccc ttatggcact
     7801 gttttcaaat ctgttgatag tccctgtggc aacggctaat ctgatactgg gatttatggc
     7861 aattttaatc ccggcattga ctccaatcct ggatctgcta cttgatattc agaggtatat
     7921 gattgtcctg atttcttcac ttccaattga agcactaact gtagcttcac caggacccca
     7981 ccagatgata ctgtatgttt ttattataat tgtagtccta aaatggaagg atatttccta
     8041 tttttatatt agggtaagaa aggtgatttt gctatacctc ctatttgtct ctgtcattgg
     8101 gttcttgggt ttcaatgagt
//
LOCUS       DASRQF010000099         2902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_271125, whole genome shotgun sequence.
ACCESSION   DASRQF010000099 DASRQF010000000
VERSION     DASRQF010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271125"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VFD79_02460"
     CDS             <1..415
                     /locus_tag="VFD79_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HZH92939.1"
                     /translation="RKLTTGIERFMNIERRGGNPMGFISEWQDNYVVKQVKNLKLPEF
                     GESEIAREEVIFFGKVQKVGFRLEIYELAKRLGLTGWVQNLPDGSVRAELQGEKERID
                     YLVGFMRSLKRARVDKFHSVQVELEDDAKGFTIKE"
     gene            complement(448..993)
                     /locus_tag="VFD79_02465"
     CDS             complement(448..993)
                     /locus_tag="VFD79_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92940.1"
                     /translation="MLPISWNSEVSIFKDNTILKQLGIAIGIPFGLLMVFLIAIRAWY
                     GVLLVAATLVLGAIFVYAVYGGKYSVDYIIDEDGIRMKSSDDHEKKTKTIGILTFLAG
                     VFSKSPTVSGAGLLSTLNDSRSISWKRIKDIQYNNNRNLIVIKGSPGEKIYLFCPPDL
                     YGTIRDALKESLENKRNSHHH"
     gene            1124..1765
                     /locus_tag="VFD79_02470"
     CDS             1124..1765
                     /locus_tag="VFD79_02470"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HZH92941.1"
                     /translation="MKLRENEAIVRGNPGLTEIKIKGVLKKSLVLLTILLLVSFVIIE
                     GVILMEGRKEEPPQVDYVIVLGARLYGDLPSPALLERLRVAKGYLLENGDSIAVVTGG
                     QGMDELIPEAHAMKKYLVENGIENERIIVEDRATSTFENMKYSRELIMDNETVSPKVL
                     IVTNSHHIFRAEFLARRQGMDPYGLPAKIPPSIVLQSYVREYFAVVKSLIFDH"
     gene            1780..2730
                     /locus_tag="VFD79_02475"
     CDS             1780..2730
                     /locus_tag="VFD79_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92942.1"
                     /translation="MNRKILLVLVIVLGVMAAGCSKDITAEFIGTITEINGLTAIVLA
                     DEGEDIRRSGDLVEVSLSVSDNFEFMVGDRVKVTHKGNIQEKYPLGIETISVELLERD
                     ENPEQEPVEDDDSAYNKISDYLAEESIKAFSPYYELLEFKITDYKEEIVDGKTQAEFL
                     YTIISKNYDRDPDTAGYIKEAKESGSEYYQQMYDEYLQPREMNFDFKVVMDEEDNMTL
                     YSNVSPNGVEWEETRITDYILTEETGFTGIGYDLDYKDKIFPGMSDKEVSAVISRILD
                     AVRLETDKGEVFQLTEEIAEEYGIQGLDPEYMDLLEIMVR"
BASE COUNT          922 a          463 c          737 g          780 t
ORIGIN      
        1 aagaaaacta acaaccggta ttgaacggtt tatgaatatt gaaaggcggg gaggtaatcc
       61 tatgggcttt ataagcgaat ggcaggataa ttatgtggtg aagcaggtga aaaacctgaa
      121 gctaccagag tttggtgaat ctgagatagc aagggaggaa gttatatttt ttggcaaggt
      181 gcagaaggtt ggttttagac ttgagatcta tgaactggca aagagacttg gacttacagg
      241 atgggtccaa aatcttcccg atggcagtgt aagggcagag cttcagggtg agaaggaaag
      301 gattgattat cttgtagggt tcatgaggtc attaaaaagg gccagggtag acaagttcca
      361 ctcagttcaa gtggaacttg aggacgatgc aaaaggattt accataaagg aatagcgtta
      421 aaaaatcagg cctgggcctg atttttttta gtgatgatgg ctgtttcttt tgttttcaag
      481 agattccttt aaggcatccc ttatggttcc gtaaaggtca ggaggacaga agagatagat
      541 tttttcacct ggcgagccct ttatgacaat aagatttcta ttgttgttgt attgaatatc
      601 cttgattctt ttccatgaaa tactcctgct gtcatttaag gtggacaaca gacctgcgcc
      661 tgaaacagtt gggctcttag agaatacccc cgccagaaag gtcagaattc ctatggtctt
      721 ggtctttttt tcgtggtcat cagaagattt cattctgata ccgtcttcat caatgatgta
      781 gtcaacggag tatttacctc catagacagc ataaacaaaa atagctccaa gaaccagggt
      841 tgcagctaca agaagaactc cgtaccatgc cctaattgct attaggaata ccatgagcag
      901 accaaaggga atacctatgg caatccccag ctgctttaga attgtattat ccttgaatat
      961 tgatacctct gaattccaac tgattggcaa catattatac acctcactta atatattccc
     1021 tcagctatta tttttaagca aaatccaaac aaatttgtat ttatagtgct tttaaggatt
     1081 attgtaattc tattgtaatc aaatatgtgc acgtttgggt ataatgaaat taagagagaa
     1141 tgaggcaata gttaggggga atcctggatt gacggagata aagataaaag gcgtactaaa
     1201 gaaatcactt gtccttctta caattctatt actagtttcc ttcgttataa tagagggtgt
     1261 aatacttatg gagggaagaa aggaagagcc gcctcaggtt gattacgtaa tagtacttgg
     1321 agcaaggcta tatggagact tgccttcccc cgcgctcctg gaaaggctaa gggtggctaa
     1381 gggatatctt ctggagaatg gagattcaat agctgttgtt acaggtggac agggtatgga
     1441 tgagttgatt ccggaagctc atgcaatgaa gaagtacctg gttgaaaatg gaatagaaaa
     1501 cgagaggata atagtagagg acagggctac atcaaccttt gaaaacatga agtactccag
     1561 ggaacttatc atggataatg aaacggttag cccaaaggtc ctaattgtaa ccaacagcca
     1621 ccacatattc agggcggaat ttctggcaag aaggcagggg atggatccat atggcctacc
     1681 tgctaagatc ccgccatcca tcgttttgca gagctatgtt agagagtatt ttgcagttgt
     1741 taagtcactt atatttgatc attaaggagt gagtgtaaaa tgaacaggaa gattttgctg
     1801 gttttagtaa tcgtactggg agtcatggcg gcaggctgca gtaaagacat tacagcggag
     1861 tttattggaa ccatcactga gatcaacggt cttacggcca ttgtattggc agatgaggga
     1921 gaggatataa gaagatccgg agacctagta gaggtaagcc tttcggtaag cgacaatttt
     1981 gaattcatgg tcggagacag ggtcaaggtt actcataagg ggaatataca agaaaaatat
     2041 ccactaggga ttgaaaccat atctgtagaa cttttggaaa gggatgagaa tcctgaacag
     2101 gagcctgttg aagatgatga ttccgcatac aacaagataa gtgattatct ggcggaggag
     2161 agcataaagg ctttctcccc ttattatgaa ctgctggagt tcaagataac ggactataag
     2221 gaagaaattg ttgatggcaa gacccaggcg gaattcctgt atacaataat aagcaagaac
     2281 tatgacaggg atcctgatac agcggggtac ataaaagagg caaaggaaag cgggagcgag
     2341 tactatcaac agatgtatga tgagtatctg cagcctaggg aaatgaactt tgacttcaag
     2401 gtggtcatgg acgaagagga caatatgacc ctatattcca atgtctctcc aaatggtgtg
     2461 gagtgggaag agacaaggat aaccgattac atactgacag aagagactgg attcacagga
     2521 atcgggtatg accttgatta caaggacaag atattcccag gaatgtcgga taaggaagtc
     2581 agtgctgtca taagcaggat attggatgca gtaaggctgg agaccgacaa gggtgaggta
     2641 ttccagttga ctgaggaaat tgcagaggag tatggaattc agggactgga tccagagtat
     2701 atggatttgc tggaaattat ggttagatag ctaaatacag ctattggttt tgtaaattgt
     2761 ggagtgtgag attaaagctt aggaggattt cgtgaggacc aaaaatgtta ttgttgaacc
     2821 ctacaactca aagtggaagg aagaatttga gaggcttaag tcatattatg agagtcatgt
     2881 tgggagaatg attattagta tt
//
LOCUS       DASRQF010000100         6708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_559373, whole genome shotgun sequence.
ACCESSION   DASRQF010000100 DASRQF010000000
VERSION     DASRQF010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6708
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559373"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1848
                     /locus_tag="VFD79_02480"
     CDS             <1..1848
                     /locus_tag="VFD79_02480"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009430364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HZH92943.1"
                     /translation="WNMTDLQRDIYEKKYRYDSETFDDFLTRVSGNNNPVKKAIKDKK
                     FMPAGRILAGRGLDKLGRKITLSNCYVMPKVEDNIESIFDTAKYLARTYSYGGGVGLT
                     LSKLRPKGSKVNNAASTTTGAVSFMDLYSMVTGLIGMRGRRGALMLNLDCNHPDIEDF
                     INVKNDLSKVTYANISVNVDDKFMQAVKDDTDYELYFKVESTGEEMSKVIRARDLYKK
                     LAVNNWNMAEPGILYKDKIDSWHLMSHDPTFEFAGVNPCAEETLPAFGSCNLSSINLS
                     EYVKHPFTKYARFEFEKFGEMVRNGVIYLNEVLDENMNLHPLEQQREMSRDLRQIGLG
                     IMGLADMFIKMGVRYGSSESISLIHQIGRIMVNEALRQSALLAEKDGPFPRFNLDAVL
                     ASPFLKENADEDVLEMIKEHGLRNSQLLTIPPTGSISTLVGCSNGVEPIFQVSYTRKS
                     ESLHHEDTYYKVFTPIIKEYMEENNLSYEEELPDFIVTTSNLEYKERIEVQATWQQYI
                     DASISSTVNVPNEFTVDQVEELYLYAWERGLKGITIYRDGCARSGILITNNKKSNIDK
                     IEELQEEIMQLAEEQLKEDPDTCPMCGGKLNHSGGCSECQDCGYSPCSI"
     gene            2182..3108
                     /locus_tag="VFD79_02485"
     CDS             2182..3108
                     /locus_tag="VFD79_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZH92944.1"
                     /translation="MRSFYLKRIVSMLITIILITTLTFTMMHSIPGGPFTRERPVPDE
                     IMRALNAKYNLDDPLPVQYLNYMKGLVTFDLGPSYSKIGTSVNDLIISGFPASAKIGL
                     IASVLIVAIGIPLGVISALKQNSPLDYFVMFLATIGITVPSFVIATVIIYFFAGKLGW
                     IPSFGLSSWKGYIGPVIALAGYSLSFVTRLTRSSMLEVLRQDYITTARANGIKEFTVI
                     MKHAVRNALIPVVTYIGPMVAAILTGSFVIEKIFAIPGIGRHFVESVSNRDYTTIMGI
                     TVFYAVFYLIMIFLVDVAYAFIDPRIKLKEDK"
     gene            3112..4299
                     /locus_tag="VFD79_02490"
     CDS             3112..4299
                     /locus_tag="VFD79_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZH92945.1"
                     /translation="MSKDKFVFIEQTGEDNEAIRPSLTYWQDAWRRLRKNKLSMMGIV
                     VVILIILFGTIGPNFTDYSYSDQVNAYKNLPPTLKLYELDGRYFYVSSDYNVFHVSPD
                     GTLIERLVLDPMKRDMINKVYTYQLGEEDVILDFSYNLLPNKMGYDYDFTLELDGEVA
                     QQPAMTKLNKLFPFGTDSLGRDLLTRVMYGARISLLVAFIATIVNLFIGVVYGSISGY
                     EGGRVDNIMMRIVDIINSIPLVLYVILLMVWFRGGGLTNIIIALSSVYWVSMARLVRG
                     QMLSLKEQEFVLAARVMGVSKGKIIFRHLIPNAMGPIIVSIAMMIPSAVFTESFLSFI
                     GLGVSAPMASWGTLANNALSGLTTYPYQLFFPALAIAFTMLAFNFIGDGLRDALDPRL
                     RKG"
     gene            4312..5331
                     /locus_tag="VFD79_02495"
     CDS             4312..5331
                     /locus_tag="VFD79_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZH92946.1"
                     /translation="MENNKDYILEVNGLKTSFYTHVGEVQAVRGIDFKLKSGDVMGIV
                     GESGSGKSVTARSVMKLIEHPGVIKEGSIIFDGKDITGFSDQKMSNIRGNDMAMIFQD
                     PMTSLNPVFRIEDQMTEVIRRHQKVSHREAKEKAIEMLHLVGIPEPAKRIRSYPHEFS
                     GGMRQRVMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMRDISTKLDTAIILITHD
                     LGVIAETCTDLVVMYGGMPMEKGSVEDIFESPQHPYTRGLLKSVPRMDTEEKGRLKPI
                     AGSPPDLLSPPAGCPFSTRCPNVMQICTEQAPPMFSVGEKHTSACWLMHEDAPEVEGY
                     RKGAL"
     gene            5333..6313
                     /locus_tag="VFD79_02500"
     CDS             5333..6313
                     /locus_tag="VFD79_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZH92947.1"
                     /translation="MEERKPLLQLQNLKKYFDVSTGFFKREQAYLTAVDDVSFDIYKG
                     ETFGLVGESGCGKSTLGRSVVRLYEPTGGKIIYDGDDITNLSQNEMMKYRKRLQMIFQ
                     DPYASLNTRLTVSEIIGEGIETHNLYSGKEKLEKIYDLLQTVGLKREHASRYPHEFSG
                     GQRQRIGIARSLAVEPELVVCDEPISALDVSIQAQVVNMLEDLQNDMGLTYLFIAHDL
                     SMVKHISDRIGVMYLGKMMEITTSVELYKNPLHPYTQALLSAIPIPDPKQNKMHKRII
                     LQGDVKSPINPPEGCRFSSRCRYCMDICTEVTPELREVEPGHFVACHLVK"
     gene            6450..>6708
                     /locus_tag="VFD79_02505"
     CDS             6450..>6708
                     /locus_tag="VFD79_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92948.1"
                     /translation="MNKKLLALMLVLAMSITVLAGCSQPAEEPAEQPAEQPAEQPAEE
                     PASGESVFHWNIGADPKTIDPVLNGASDGGDVINQTFEGLVR"
BASE COUNT         2066 a         1346 c         1640 g         1656 t
ORIGIN      
        1 tggaacatga cagaccttca gagggatatt tacgagaaga agtacagata tgattcagaa
       61 acctttgatg acttccttac aagggtgagt ggaaacaaca accctgtaaa gaaggccata
      121 aaggacaaga agtttatgcc ggcaggaaga atccttgcag gccgtggact ggataagctg
      181 ggtaggaaga taaccttgag caactgctat gttatgccaa aggttgaaga taacatagaa
      241 tcaatatttg atactgcaaa gtatcttgca agaacctatt cctacggtgg tggggttgga
      301 cttacccttt cgaagcttag accaaagggg tcaaaggtca ataatgcagc aagcacaact
      361 actggagcag taagctttat ggacctttat tcaatggtta cgggacttat aggcatgagg
      421 ggaagacgag gggcattgat gctaaacctt gactgcaatc atccggatat tgaagatttt
      481 ataaatgtta agaatgacct tagcaaggta acctatgcca atatctccgt aaacgttgat
      541 gataagttta tgcaggcagt aaaggatgac accgactatg agctctattt caaggttgaa
      601 tcaaccggtg aggaaatgag caaggtcatt agggcaaggg acctttacaa gaagcttgcg
      661 gtcaacaact ggaacatggc agaaccggga atactctaca aggacaagat tgattcatgg
      721 cacctgatga gtcatgaccc cacgttcgaa ttcgcagggg taaacccatg tgcagaagag
      781 acccttcctg catttggaag ctgcaaccta tcctcgatca accttagcga atacgttaag
      841 catccattta caaagtacgc cagatttgaa tttgagaagt ttggtgaaat ggtaagaaac
      901 ggcgtcatct atctaaacga ggttctggat gagaacatga accttcaccc actggagcag
      961 caaagggaaa tgtccaggga cctgagacag atcggacttg ggataatggg tctggcagac
     1021 atgttcatca agatgggagt aaggtatgga agcagcgaat cgatctccct gatccaccag
     1081 atcggtagga tcatggttaa tgaggccttg agacaatcag ccttactggc ggaaaaggat
     1141 ggtcctttcc caaggttcaa cctggatgcg gttctagcat ctccattctt aaaggagaat
     1201 gcagacgagg acgtactgga gatgataaag gagcatggac tgagaaattc acagctcttg
     1261 acaatccccc caactggaag catatccaca cttgtgggct gcagcaacgg ggtggagcca
     1321 atcttccagg tgtcatacac caggaaatcg gaatcactac accacgagga tacctactac
     1381 aaggtgttca cgccaatcat caaggagtat atggaggaga acaacctgtc atacgaggaa
     1441 gagctacctg atttcatagt tacaacctca aaccttgaat acaaggagag gatagaggtc
     1501 caggccacct ggcagcagta tatagatgcc agcatctcat ccacggtaaa cgtaccaaac
     1561 gagttcactg tggaccaggt agaggagcta tacctctatg cttgggagag gggacttaag
     1621 gggattacca tctacaggga cggatgtgct agaagtggaa tccttattac aaacaacaag
     1681 aagagcaaca tagacaagat agaggagctg caggaggaaa taatgcagct tgcagaggag
     1741 cagcttaagg aagatccgga tacctgtcca atgtgtggtg ggaagcttaa ccactcagga
     1801 ggatgctcag agtgtcagga ctgcggctac agcccctgtt caatatagga gagagtagta
     1861 aatgctgaag gacctgttgg tccttcagca tttttttaat gaaagatttg tgaaaatttt
     1921 ttaaataatg gaatatttaa tcaaatgtct acaattttca tcttgtggaa ggtgtgcaaa
     1981 ggcttgcata agggcttgaa atgtcttgat atacagcgtg gctttaccca aacaaagcat
     2041 cagtgggata gcggcacaat agctaaaagc gctttttcac taggattgag ttgaaaaagt
     2101 attttgaaaa atcagtgcaa agatggtaga ataaggtaga gtatttgcaa ctcaaaaaat
     2161 acatagaaag gagttacaat tttgagatct ttttacttaa aacgcattgt atcaatgttg
     2221 ataactatta tactgatcac tacattgacc tttacaatga tgcactcaat cccaggaggc
     2281 ccctttacac gggaaagacc agtgcctgat gagatcatga gagcattgaa cgccaagtac
     2341 aacctggatg acccactgcc agttcagtat ttgaattaca tgaagggtct ggtgacattt
     2401 gacttggggc catcctattc aaagattggt acatcagtca acgatttgat catttcagga
     2461 tttcccgctt cggcgaaaat cggcttgatt gcatcggtgc tgatagtggc cataggaata
     2521 ccattggggg ttatatcagc acttaaacag aacagtccac tggactactt tgtaatgttc
     2581 ctggccacaa tagggatcac tgttccaagt tttgtaatag caacagttat aatatatttc
     2641 tttgcaggta agctggggtg gattccaagc tttggactga gcagctggaa aggctacatt
     2701 ggtccggtaa tagcccttgc aggttattca ctgtcctttg taaccagact tacaagatca
     2761 agcatgctcg aggtcttgag acaggactac atcacaaccg caagagccaa tggaatcaag
     2821 gaattcacag taataatgaa gcatgctgtc agaaatgccc tgattccagt agtaacctac
     2881 attggaccca tggttgctgc aatccttacg ggatcatttg taatagagaa gatttttgcg
     2941 attcctggaa taggaagaca ctttgttgaa agtgtcagca accgtgatta cacgacaata
     3001 atgggtatca cagtattcta tgctgtgttc tacctgataa tgatattctt ggtggatgtg
     3061 gcttatgcct tcatagatcc acgtatcaag ctgaaggagg ataagtagac catgtcaaag
     3121 gacaaatttg tttttattga acagacagga gaagacaacg aagccatcag acccagtctt
     3181 acttattggc aggatgcatg gagaaggcta agaaagaaca agctgtccat gatgggcata
     3241 gttgttgtca tccttattat actgttcgga accattggac cgaattttac ggattattca
     3301 tattcagacc aggttaatgc atacaagaac ctgccaccga cccttaagct ttacgagcta
     3361 gatggaagat acttctacgt ttcaagtgac tacaacgtat tccatgtttc accggatgga
     3421 acactcatag aaagacttgt gcttgacccc atgaagaggg acatgatcaa caaggtatat
     3481 acctaccagc tgggagaaga ggatgtaata cttgacttct cctacaacct tcttccaaac
     3541 aagatgggct atgactatga tttcaccctg gagctggatg gggaggttgc acagcagcct
     3601 gcaatgacaa agcttaacaa gcttttccca ttcggtacgg attcacttgg aagggacctt
     3661 ttgacaaggg taatgtatgg agcgagaatt tcactactgg ttgcgtttat agccaccata
     3721 gtcaacctgt ttatcggagt tgtttacgga agcatctcag gatatgaagg cggaagagtt
     3781 gacaacataa tgatgagaat tgtggatatc ataaactcca taccacttgt actgtatgtt
     3841 atcctcctga tggtgtggtt taggggtgga ggacttacaa acatcataat cgccctaagt
     3901 agcgtttact gggtatccat ggcaaggctt gtgaggggac agatgctgtc acttaaggag
     3961 caggagtttg tccttgccgc cagggttatg ggagtgtcaa agggaaagat aatcttcaga
     4021 caccttattc caaatgcaat gggacctatt atagtttcca tagccatgat gattccttca
     4081 gctgtattta ccgagtcatt ccttagcttt ataggacttg gggtatcggc accgatggca
     4141 tcatggggta cgcttgcaaa caatgctctt tcaggtctta caacataccc gtatcagttg
     4201 ttcttcccgg ctttggcaat cgcatttaca atgctggcat tcaactttat cggtgacgga
     4261 ctgagagatg cactggaccc aagacttaga aaaggatagg gagattgaga tatggaaaac
     4321 aataaagatt atatattaga ggtaaatgga ttaaaaacct ccttctatac ccatgtggga
     4381 gaggtccagg ccgtccgtgg catagacttc aaactgaaaa gcggagatgt aatggggata
     4441 gttggagaat cgggtagtgg aaaaagcgtt actgcccgtt ctgttatgaa gcttatagaa
     4501 cacccgggag ttatcaagga aggcagtata atatttgatg gaaaggacat tactggattc
     4561 agcgaccaaa agatgtccaa catcagaggt aatgacatgg ccatgatctt ccaggatcca
     4621 atgacttcat tgaacccggt gttcaggata gaagaccaga tgacagaggt aatcagaaga
     4681 caccagaagg taagccatag ggaagccaag gaaaaggcca tagaaatgct tcaccttgtt
     4741 gggatacctg agcctgcaaa gagaataagg tcatatccac acgaattttc gggtggaatg
     4801 agacagaggg ttatgattgc aatggccctg tcctgcaatc ccaagcttct catagcggat
     4861 gagccgacaa ctgctcttga tgttacaatt caggcacaga tccttgacat aatgagagat
     4921 atctccacaa agcttgacac ggctatcatc ctgatcaccc atgaccttgg tgtaattgca
     4981 gaaacctgca cagaccttgt tgtcatgtat ggggggatgc caatggagaa gggaagcgtt
     5041 gaggatattt tcgaaagtcc tcagcaccca tatacgagag gcctcctaaa gtcagtgcct
     5101 agaatggata cagaggaaaa aggaagattg aagccaattg ccggatcacc cccggacctt
     5161 ctgtcgccac ctgcggggtg tcccttctca acaagatgtc caaacgttat gcagatttgc
     5221 acggaacaag cacctcccat gttcagcgta ggggagaaac acacttcagc ctgctggttg
     5281 atgcatgagg atgcacctga ggttgaaggc tacagaaagg gggctttata atatggagga
     5341 gagaaagcca cttctacaat tacagaacct aaagaaatac ttcgatgtaa gtacgggctt
     5401 ctttaaaagg gagcaggcat acctgactgc cgttgacgat gtttcctttg atatctacaa
     5461 gggagagacc ttcggtcttg ttggtgagtc cggctgtgga aaatcaaccc ttggaagaag
     5521 cgttgtaaga ctttatgagc caactggagg aaagataata tacgatgggg atgacataac
     5581 aaacctatcc cagaatgaga tgatgaagta caggaagaga cttcagatga tcttccagga
     5641 tccatatgca tctcttaaca cacgtttgac tgtttcagag attatcggag agggtataga
     5701 aacccataac ctgtactccg gcaaggagaa gctggaaaag atatatgacc ttttacagac
     5761 agtaggcctg aagagagagc acgccagcag gtatccccat gaattctcag ggggacagag
     5821 acagcgtata ggtattgcaa ggtcccttgc tgtcgagccg gagcttgtgg tgtgtgacga
     5881 gcctatatca gcccttgacg tatcaataca ggcacaggtt gtaaacatgc ttgaagacct
     5941 gcagaacgat atgggactta cttacctgtt catagcccac gacctgtcaa tggttaagca
     6001 tatatccgac cgtataggtg taatgtacct tggaaaaatg atggagataa caaccagtgt
     6061 cgagctttac aagaatccac tccatccata cacccaggcg cttttatcag ccatacccat
     6121 ccctgatccc aagcagaaca agatgcacaa gaggatcatc ctccagggag atgtgaaaag
     6181 tccgataaac ccacctgaag ggtgtagatt cagttccaga tgccgctact gcatggacat
     6241 ctgtactgag gtgacaccag agcttagaga ggttgagcca ggtcattttg tggcctgcca
     6301 tcttgtcaaa taatgaattt gtggtataat agagtatatt aaaaattttc tcaatattta
     6361 ccacgtttgt gcagggactt aaagaagtat ttaatccagg atctgcctgg tttaaataag
     6421 gcaacataca tcattaagga ggggaaaaaa tgaacaagaa actattagcg ttaatgttgg
     6481 ttttggcaat gtcaatcaca gtattggctg gatgtagcca accagcagaa gaaccagcag
     6541 agcaaccagc tgagcagcca gcagaacagc cagcagaaga gcctgcatca ggagagtcag
     6601 ttttccattg gaatataggc gctgacccta agactatcga ccctgttctt aacggagcaa
     6661 gtgacggtgg agacgtaatc aaccaaacat ttgagggact agtaagag
//
LOCUS       DASRQF010000101         2482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_146454, whole genome shotgun sequence.
ACCESSION   DASRQF010000101 DASRQF010000000
VERSION     DASRQF010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146454"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="VFD79_02510"
     CDS             complement(<1..1138)
                     /locus_tag="VFD79_02510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HZH92949.1"
                     /translation="MKELVSKYYREVIDLNDFMADNPEIGGQEYQSSKRMVHLLESHG
                     IPVEYPFAGMDTAFRGIINKGMGRKAAILVEYDALRGLGHACGHCASGSISILAALIL
                     NELKDRIPAEIHIIGTPDEEMHGGKVPMVKSGVFNDMDFAIMIHMSNKNALFSNFLAL
                     DAYRFTFQGMPAHASSIPWEGRNALNSMRLFFDAMDMMRQHLKDDVRLHGYIVKGGDA
                     SNIVPHYTEAEVLVRAAKREYLDQVSKWVMDCAQAAALATRTSWNVEEVGEKYDSLRR
                     NIGGERVLEGIYQDLGLTLVDTSHETGGSSDIGNVSSLCPAFHPYLDIGKDLNAHTEE
                     FAQAMKTKKTHRAIALGGEIISRFTLDVYNTPGLLEEIRKDHLGE"
     gene            1299..1736
                     /locus_tag="VFD79_02515"
     CDS             1299..1736
                     /locus_tag="VFD79_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZH92950.1"
                     /translation="MKKILVPIDGSDCSKKALMKAKDLGKAFGSHITILTIIDSIRYL
                     DMDFKFDAVRDGIDLSKQLLVTALEDFKDYPGEVETLYKTGDVAEEIIDVAEEGGFDL
                     IVMGSRGLGVFSRTILGSVSHKVIQHSKTTVMVVKDCGQVDDQ"
     gene            1891..>2482
                     /locus_tag="VFD79_02520"
     CDS             1891..>2482
                     /locus_tag="VFD79_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005956933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HZH92951.1"
                     /translation="MKTKKQATIGHALIPIIVLVASLFVGIVYLGTDAHIPILIAIIV
                     AAAVGIWGLGFTWGEIEKGAIETIQMSMQAILILMVIGTVVGSWILSGTVPAMIYYGL
                     QILSPGIFLVATAIICAIVSLATGSSWTTAGTVGIALMGVGQGLGMPAYIVAGAVVSG
                     AYFGDKISPLSDTTNLAPAMAGSTLFEHIKYMFWTTI"
BASE COUNT          669 a          553 c          544 g          716 t
ORIGIN      
        1 attccccaag atggtctttt cggatttcct ccagaagtcc aggtgtattg tagacatcca
       61 aggtaaacct ggagataatt tcccctccaa gggctatagc cctatgggtc ttttttgtct
      121 tcatggcctg tgcaaactcc tcggtgtgtg cattcaggtc cttgccaata tcaagataag
      181 gatggaaggc cggacatagg ctgcttacat ttcctatatc agacgaacct cctgtctcat
      241 ggctagtatc cacaagggtc aggccaaggt cctggtagat tccttccaac accctttctc
      301 cgcctatgtt tctccttagg ctgtcatact tttcacctac ctcctcaaca ttccatgagg
      361 ttctagttgc aagtgcagct gcctgtgcac aatccattac ccacttgctg acctggtcaa
      421 gatactccct ctttgcagcc cttacaagga cctcagcctc agtgtaatga ggaacgatgt
      481 ttgaggcatc tccccccttg acaatatagc catggagtct cacatcatcc tttagatgct
      541 gcctcatcat atccatggca tcaaagaaga gccgcattga attcagggcg tttctacctt
      601 cccatggtat gctggaagcg tgtgcgggca ttccctggaa ggtgaacctg tatgcgtcaa
      661 gagccagaaa atttgagaaa agagcattct tgttgctcat gtggatcata attgcaaaat
      721 ccatgtcatt gaagacacca gatttgacca ttggaacctt gcccccatgc atctcctcgt
      781 ctggagttcc tattatgtgg atttcagctg ggatcctgtc cttcagctca tttagaatca
      841 aggctgcaag aatgctgata gatccacttg cacagtggcc gcaagcatgc cccaaacccc
      901 ttagtgcatc gtactcaaca agtattgcag ccttcctgcc cattcccttg ttgattatcc
      961 ccctaaaggc tgtgtccatt cctgcaaatg gatattcaac tggaattcca tggctttcaa
     1021 gcagatggac catcctcttg gaggattggt attcctggcc accgatttcc ggattatcgg
     1081 ccataaaatc atttaagtct attacttcac ggtagtattt actgactagt tctttcattt
     1141 caccctcccc ttgtgattag tcttatctat ttaacctatc atagcaaata tgagccctca
     1201 ggcatagagt aaacatgtct tcccttcgta aacaatcaat atattcagat tatattgggt
     1261 atatagggat ggaatggaaa atatatggag gtgtgacaat gaaaaaaatt ctagtaccaa
     1321 ttgatgggtc cgactgctca aaaaaggccc ttatgaaggc aaaagacctg ggtaaggctt
     1381 ttggttccca cattacaatc ctaaccatca tagacagtat caggtatctt gacatggatt
     1441 ttaaattcga tgcggtcagg gatggcattg accttagcaa acagcttctc gtaactgcat
     1501 tggaggattt caaggattac ccaggggagg ttgaaacact gtacaagacc ggtgatgtgg
     1561 ccgaggaaat aatagatgtg gctgaagagg gtggcttcga ccttatcgtt atgggcagca
     1621 ggggtcttgg tgttttttca aggacaattc ttggaagcgt atcccacaag gtaatccagc
     1681 actcaaaaac cactgtaatg gtcgttaaag actgtggtca ggtggatgac caataagtcc
     1741 ccaccttagt tgacctgctt gaatattcat ctaaattgtc agaaaatgat tgaatttttt
     1801 ctgaaaatag tgtaatctat aatagaatct gtgcgtaaaa cacatagggt gtgaaaacgt
     1861 attcagatac taatatttag gggggtttat atgaaaacga agaaacaagc aacaatcggt
     1921 catgcattaa tacctattat cgttctagtg gcatctttgt ttgttggtat cgtttatctg
     1981 ggtactgatg cacatatccc aatactgatt gcaatcatag ttgcagcggc agtgggaatt
     2041 tggggccttg gctttacctg gggtgaaatt gagaagggtg caattgaaac cattcagatg
     2101 tcaatgcagg caatattgat acttatggtt atcggtacag ttgtagggtc ctggatcctt
     2161 agtggaacag ttccagcgat gatctactac ggccttcaga tcctttcacc agggattttc
     2221 cttgtggcaa cagcaataat ttgtgcaatc gtatcacttg caacaggtag ttcatggaca
     2281 actgcaggta ctgtaggtat tgcacttatg ggagttggac aaggacttgg aatgccagca
     2341 tacatagtag caggtgctgt tgtatccgga gcatacttcg gagacaagat atcacctctt
     2401 tcagacacaa caaaccttgc accggcaatg gctggatcaa cattgttcga gcatatcaag
     2461 tacatgttct ggacaacaat ac
//
LOCUS       DASRQF010000102         5155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_518735, whole genome shotgun sequence.
ACCESSION   DASRQF010000102 DASRQF010000000
VERSION     DASRQF010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518735"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /locus_tag="VFD79_02525"
     CDS             <1..1247
                     /locus_tag="VFD79_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HZH92952.1"
                     /translation="SLLEKVFRNFGYNMTVLKNTGPSVVDQGLRNVHNDTCYPALLVI
                     GQMIDALNSGEYDTDRVALLITQTGGGCRASNYIHLLRKALIKAGYDHVPVISLNAGG
                     MEKNSGFKVSFPMLKQSMASLIYGDLLMLLYNQTRPYEKVQGESRRKLDMWIDRLGEE
                     FQSGRTIKQREMKDLMKEIVLSFENIEREEKSKVKVGVVGEIYVKFSSLGNNNLEEFL
                     HREDCEVMVPGLMGFIMYSVENGVIDQQLYGGRFWKSVVMDRINRYLGGLENIMIQAV
                     QNNSRFIAPSPFKHLKSLVDDVIGLGCKMGEGWLLPAEMMELVSKGYENIVVTQPFGC
                     LPNHIVGKGIVANIKRIHPMANIVPIDYDPGATRVNQENRIKLMLSVAREKLDHVKTS
                     AANRIEESLVEPGADKLPVKMI"
     gene            1330..2094
                     /locus_tag="VFD79_02530"
     CDS             1330..2094
                     /locus_tag="VFD79_02530"
                     /inference="COORDINATES: protein motif:HMM:NF017927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HZH92953.1"
                     /translation="MWSRVGLKDQAKDFLRRNYWKAFVVCLIVAIVTGGSGRSESRSE
                     FNNGMYNIEREYIVDFSPGNDIFNITTNGIGPGNPFVFNTTLGIFAAFLVFSVIIGII
                     IGNALTVGEKRFFLRGFEGDVSIGNLFSTFKKGQWMPLAGKILLLDIYILLWTLLLII
                     PGIIKYYQYRMVPYILSEDPSMPLGQAIEISRRMTDGEKGEIFVLDLSFLGWYLLGLL
                     FFGIGSIFVRPYHEATVAKLFEHYVSRGEPEEIVEY"
     gene            complement(2137..3768)
                     /locus_tag="VFD79_02535"
     CDS             complement(2137..3768)
                     /locus_tag="VFD79_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92954.1"
                     /translation="MNNKIYYLLLLTIIAGLVLSGCGSGNQEMTTMSVEDSVESFFPF
                     DQSLLESDFSREKIENYILSINPNVNTKAGHYAIGDLTGDDIPEIAMYIERDPKNIDD
                     QGSLLVYTYDSSTYLMTDRTPMNYDNTNYILKIGNLDEGTSGILLSNQVGSKAGVTYG
                     YILEDGRLKSVLNPKKVNLFSVTTSNRVEDIDGDGILEFSIHSIDPETSPGEPSEAET
                     IELWYKWNGKDSADVISVAGASIQNTPMMASRKESGLSDNEDSFSIMSSPEDSGPAQS
                     QVDEPSPGTVEYLDHLMEIKDGYTSRELTDRLEDHLASLQVNKGYRSLDIATMFSKYV
                     KEYTFDSFFQKYGLSQERLNDMEYLQRDRILQSEPDLKEMLIRNRRLGYILIVESDRY
                     NYVIDSKMFIDGFGGNMTNEFRKYLQIMSREASLPHTREGILRISKEELATRIVEIEG
                     FRLTYSYSKFLDEVLELYQDYMNSILYITGDGLVFNQDTGVFVGDSRQGLQDIVNNHP
                     DTNMSDVINLMLQEVKRSSGAITPATRDKIASMIP"
     gene            3906..4547
                     /locus_tag="VFD79_02540"
     CDS             3906..4547
                     /locus_tag="VFD79_02540"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HZH92955.1"
                     /translation="MLLGELREKVVEYGKKLIEKNLTTGTGGNISIFHRESGLMLMSP
                     SGIDYMETRANDVVVMNLIGDIVDGDRKPSSEYDLHRIFYLNREDISALVHTHSMYAT
                     TIACMNWDLPPVHYMVAVAGDNVRCAGYATFGTMELAENAYRAMENRKAVLLANHGML
                     AGANNLDQAFKIAEDIEFCCELYYRTKSLGEPVIIDQDEMVRMRDKFRTYGQR"
BASE COUNT         1475 a          996 c         1256 g         1428 t
ORIGIN      
        1 tctcattgct tgagaaggtg ttcaggaact ttggctacaa catgactgtt ctaaagaaca
       61 cagggccttc cgtggttgac cagggcttga ggaatgtcca taatgacacc tgttatcctg
      121 ctcttcttgt aatcggtcag atgattgacg ccctcaattc aggtgaatac gacacagaca
      181 gggttgccct tctcataacc cagactggtg gtggctgtag ggcatcaaac tacatacacc
      241 ttctgagaaa ggcactgatc aaggcaggct atgaccacgt tccggtaata tccctaaatg
      301 caggagggat ggaaaagaac agtggattca aggtttcctt tccaatgctt aagcagtcca
      361 tggcaagtct aatctatgga gaccttctta tgctcctata caaccagaca aggccctatg
      421 agaaggtcca gggggaaagc aggaggaagc tggatatgtg gatagacagg ctgggggagg
      481 aattccagtc aggaaggacc ataaaacaaa gggaaatgaa ggacttgatg aaggagattg
      541 tgctttcctt tgaaaatatt gaaagggagg aaaagagcaa ggtaaaggta ggagttgtag
      601 gagagattta cgttaagttc tccagtctgg gaaacaataa cctggaggaa tttctccaca
      661 gggaggattg cgaggttatg gttccgggct tgatgggctt tatcatgtac tcggttgaga
      721 acggtgtaat tgaccaacag ctctacgggg gcaggttctg gaagagcgtt gttatggaca
      781 ggataaaccg ctatcttgga ggccttgaga acataatgat tcaggctgtc cagaataaca
      841 gcagatttat agctccttcc ccattcaagc atctgaagtc actggttgat gatgtcatag
      901 gactgggctg taagatggga gagggatggc tccttccagc tgaaatgatg gagctagtat
      961 caaaggggta tgaaaacatt gttgtaaccc agccctttgg atgcctacca aaccacatag
     1021 taggaaaggg tattgtggct aacataaaga ggatacaccc catggccaac attgtgccaa
     1081 tagattacga cccgggtgcc acaagggtca accaggagaa caggatcaag cttatgctgt
     1141 ccgttgccag ggaaaagttg gaccatgtca agaccagtgc agccaataga atagaggaat
     1201 cacttgtgga gccaggagca gacaagcttc cagtcaagat gatttaaggg aggggtctga
     1261 ctaaaaatca ggcccttttc attgacagga gattaacaag gtgttagtat tgagctggag
     1321 gtggataaaa tgtggtcacg agtgggttta aaggatcagg caaaggattt tctaaggcga
     1381 aattactgga aggcttttgt ggtttgccta atagttgcaa ttgttacagg cggctcagga
     1441 agaagtgagt ccaggagtga attcaataat ggaatgtaca acattgaaag ggagtacatt
     1501 gtagattttt caccgggaaa tgatattttc aacattacaa ccaatgggat agggcctgga
     1561 aatccctttg tattcaatac aacactgggt atattcgcag cgtttttggt attctcagta
     1621 ataataggaa ttataattgg aaacgccttg acggttgggg agaagaggtt tttcttgagg
     1681 ggctttgaag gtgatgtaag tattggaaac ctattttcaa ccttcaagaa gggtcagtgg
     1741 atgccccttg caggtaaaat ccttcttttg gatatttaca tcctactttg gacattgctc
     1801 ttgataatcc ctggaatcat taagtactac cagtacagga tggtccccta catattgtcg
     1861 gaggacccaa gcatgccttt gggtcaagca atagaaataa gtagaaggat gaccgacggt
     1921 gagaagggag aaatatttgt tctggacctt tcatttcttg gatggtacct gctgggtctt
     1981 ctcttcttcg ggataggtag catatttgta aggccctacc atgaggccac tgtagcaaag
     2041 ctttttgagc actacgtaag caggggagag ccagaagaga tagttgagta ctaaaaaaag
     2101 gaatcctgcc atcaaatcgg caggattcct catatactag ggtatcattg atgcaatttt
     2161 atcccttgtg gcaggggtga tggctccact gcttcttttg acctcttgaa gcatcaagtt
     2221 tatcacatca gacatgttgg tatcgggatg attgtttact atatcctgaa gcccctgtct
     2281 ggaatccccc acaaacactc cggtgtcctg attgaatacc aagccatccc cagtaatgta
     2341 taggattgaa ttcatgtagt cctggtaaag ctccaggact tcatccagaa acttggaata
     2401 gctgtaggtc agcctgaagc cttcaatttc aacaattctt gttgccagtt cctcttttga
     2461 aatcctaagg atgccttccc tggtgtgggg aagggatgct tccctcgaca ttatctgaag
     2521 gtattttcta aactcatttg tcatattgcc gccaaatccg tcaataaaca tctttgagtc
     2581 aattacatag ttgtacctgt cactttcgac tatcaagata tagccaagcc ttctgtttct
     2641 aataagcatc tcctttaggt cgggctcaga ctggaggatc ctgtcccttt gcaggtattc
     2701 catatcgttt agacgttcct gggaaagtcc atacttttga aagaatgaat caaaggtgta
     2761 ttccttcaca tacttggaaa acatggttgc tatatcaagg cttctgtagc ctttattcac
     2821 ctgtagtgaa gccaggtggt cttcaagcct gtcagttagt tccctgcttg tataaccatc
     2881 ctttatctcc atgaggtggt ccaaatattc cacggttcct gggctgggct catctacttg
     2941 agattgtgca ggtccagaat cctcaggaga actcatgatg ctgaagctat cctcattgtc
     3001 tgaaagtcct gattccttgc ggctagccat cattggggtg ttttggattg aagctccggc
     3061 tactgatatg acatctgcgc tgtccttgcc attccacttg taccacaatt caattgtttc
     3121 tgcctcagaa ggttcacctg gacttgtttc agggtcaatc gagtggatgc tgaactccag
     3181 gattccgtct ccatcaatgt cctctaccct attggaggtg gtgacagaaa ataggtttac
     3241 tttcttgggg ttcaagacac ttttcagcct tccatcctcc agaatataac cgtatgtaac
     3301 tccagccttg gagcccacct ggttgctaag gagtatccca cttgtacctt catcaagatt
     3361 tccgatttta aggatgtagt ttgtattgtc atagttcatt ggcgtccggt ctgtcataag
     3421 ataggttgag gagtcatagg tgtagaccaa cagcgaaccc tgatcatcta tgttcttggg
     3481 gtctctttca atatacattg ctatttcagg tatatcatca ccggtcagat caccgattgc
     3541 atagtgtcct gcctttgtgt tgacattggg atttatgctg agtatatagt tttctatttt
     3601 ttcccttgag aaatccgact ccagtaatga ctggtcaaat gggaagaagc tttccactga
     3661 atcctccaca gacattgtcg tcatctcctg atttccgctt ccacatcctg acaggacaag
     3721 cccggcgata atcgtcagaa gcaggagata atatatctta ttgttcatga tattgcccct
     3781 ttcttgttat tattatataa aaaaaacaat tgcagtgtgt aacaatcagg ttacagaaac
     3841 tgggcaattg acagactatt cagggattgt tactatgaag gtacaagaat ggaggaggaa
     3901 attacatgtt gcttggagag cttagggaaa aggttgttga gtacgggaaa aaactcatag
     3961 agaagaacct gactacgggt acaggtggaa acataagcat tttccatagg gagtcaggcc
     4021 ttatgcttat gagtccatca ggtattgatt acatggaaac cagagcaaat gatgttgtgg
     4081 tgatgaacct tatcggggat attgtggatg gtgacaggaa gccttcttcc gaatatgacc
     4141 tccacaggat attctacctt aacagggagg atatatcagc cctggtccac acccattcca
     4201 tgtatgctac gacgattgca tgcatgaact gggatttgcc acctgttcac tacatggtcg
     4261 cagtcgcagg agataatgta cggtgtgcag gctacgccac ctttggcaca atggaacttg
     4321 cagaaaatgc ctacagggcc atggagaaca gaaaggctgt gcttcttgca aaccatggga
     4381 tgctggcagg ggcaaataac ctggaccagg cctttaagat agctgaggat attgagtttt
     4441 gctgtgagct ctattacagg accaaatcac tgggtgagcc ggtcataatt gatcaggatg
     4501 agatggtgag gatgagggac aagttcagga cctatgggca gagatgatta taattcacaa
     4561 tgcacaatgc acagttcata attaaaaaaa cttagattta accaggacat ggggttagtt
     4621 gctagtagtt agtggaaagt tgaaagctga gattgtaaag tgtaaagatt tatgtaatat
     4681 ctaagatcta agaagtgaga agtaataaag ataggtgaat tattagggtc caagatcctt
     4741 cgtttcactc aggaagacaa acatactgtc actctgagca tgaagaagag tcctggaccc
     4801 tatttattta cttaacaatt gtgaactgtg aattgagaat tgtgcattga agacatttgg
     4861 ttacaaaacg gttaaaattt aataaacttc caaatccatc acattattcc aacgaaatat
     4921 gttaaaatat aaacgggtgt ataatggtga tgaattccac tgttaaagtg gaatgggcca
     4981 taaaggaaac acaagaaagg gagagattaa ttatgaagag agtattagtg gcattggttc
     5041 ttgtattggc aatgttttca acaaccatag catcagcagc tccggaaact ccggcaggag
     5101 aatactggga agagctgaag gcaatgtatg aggaatgggc agcaatggaa ggcga
//
LOCUS       DASRQF010000103         8198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1539963, whole genome shotgun sequence.
ACCESSION   DASRQF010000103 DASRQF010000000
VERSION     DASRQF010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 8198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8198
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539963"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..879)
                     /locus_tag="VFD79_02545"
     CDS             complement(199..879)
                     /locus_tag="VFD79_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HZH92956.1"
                     /translation="MGTIVNSVAVVAGALLGVFLRKGIKDQYTSTIMDGMALAVVIIG
                     VMGALEMNNLIVVLASIVVGSIIGERLDLDKRLENLGTSMEKRFGRGDSDFSKGFIMA
                     SLVYCIGAMAILGALESGLTGNHETLYAKSVLDGVSAVIFASTFGIGVAFSAVAVFLY
                     QGAITLLASSVKDLLTPEVINEMSSIGGILIMAIGINILGIKKVKIANMLPAIFIPIV
                     YYSIVALF"
     gene            complement(993..3065)
                     /locus_tag="VFD79_02550"
     CDS             complement(993..3065)
                     /locus_tag="VFD79_02550"
                     /inference="COORDINATES: protein motif:HMM:NF022812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HZH92957.1"
                     /translation="MNRKRSIVILSCFLALMLAFTGCREVVEEKVPDEPGEVVENPGK
                     PEESPQGPADIVPETDLSNVENLSDFPDLSPAQKEMLLENRFFVSPSDQAQLFYIYEL
                     NEYEKVPNFITTDSVLHTYHIFYDYLLRTLENEKFLQLAEDLTEDMLLKSIEIYEDLE
                     DVDLRVAQEKNIAYFAVAQQLLKNDLPGGIPLNALEMASGELELIETKVGFTDSVIFP
                     FQMDYSQYVPRGHYTRSEDLERYFLALMWYGQVPFPLYVDEDVRNQEQTLQGILMARM
                     VSEDEELMGKWRKIYDPTNFFVGSSDDLGIPDYSAAVEEIYGSDASYEDFSSQDLLDS
                     FYKRAESFPEPRIVGRYISTNTPVNKQFRFMGQRYVFDSQIIQELVYPILRPIPSGLD
                     VMAVLGSQRAREIQLENPDNQQWEDYPVEMERLREEFAHYTEVDWKRNLYTGWMWTLE
                     GLLGPADDHMPAFMRNQAWEDKNLNTALGSWAELKHDTVLYGKPSGAEMGGEEYFELP
                     GYVEPNIEVYERLLWLTNQSRDYLEEWDLNMDEIDHKLERFQWLLDFLIDASHKQLED
                     IPLTREEHDRLKIYGGILEDLTSSFTGQGTRWHEITSETDKNMAVISDYHTIGRDGVM
                     EAGVGPAYEIHVLVPIDGRLYLARGAVMSFHEFVWGERLTDEKWQEMIKEGTNPPMPD
                     WTESFIER"
     gene            complement(3058..3738)
                     /locus_tag="VFD79_02555"
     CDS             complement(3058..3738)
                     /locus_tag="VFD79_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92958.1"
                     /translation="MKLRGYFLTVVLMAIVALVTGCSQDKAILHARGNLTGEGDFLVV
                     LTGEDGQRYLKDLIIYDTGNGKREVFRKDISHLNPWKLMVGDVDGDGKDEISIGVYKE
                     SPLHPVMAKRPFFYNFDGEDLVPKWRGSRLSRPFVDFHLFDIDNDQVMEVVGEEYLED
                     GRQVLNTYKWREFGFEGYKETQAVKSIQKVFTFEDRLHAIVGDDSGNARYYVEEMEDG
                     LEWREYRE"
     gene            complement(3860..4741)
                     /locus_tag="VFD79_02560"
     CDS             complement(3860..4741)
                     /locus_tag="VFD79_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HZH92959.1"
                     /translation="MDYKIEKAEDKLIINGLKDFNPTHIFENGQCFRWYKEEDGSYTT
                     VAGGRLINILKDGDQIIVDGTDEKDWREYWHKYFDLDRDYGELKRELSRDPILREAIR
                     FGEGLRLLNQDPYETIISFIISANNQIPRIKNAVEAISNDLGDYIGTYRGRDYYSFPQ
                     PDRLASKTTQYIMEKYRVGFRGDRIREVSRRIAEGEFDTLCIYDTDHNDAVDILTSLP
                     GVGPKVSGCILLFAYDKPEAFPVDVWVKRVMEHFYFKRPATRRELEESGEKIFGNLAG
                     YAQQYLFYYARELGIGK"
     gene            complement(5046..6311)
                     /locus_tag="VFD79_02565"
     CDS             complement(5046..6311)
                     /locus_tag="VFD79_02565"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HZH92960.1"
                     /translation="MSKEILNPLESAQKQVKDACDALGYGESVYELLKEPKRFIEVSI
                     PVKMDDGSVKVFKGYRSMHSDAVGPGKGGIRFHPDVNPDEVKALSVWMTFKCCVTGIP
                     YGGGKGGITVDPSTLSQGELERLSRGYIQGLYKYLGEKIDIPAPDVNTNGQIMAWMVD
                     EYCKLTGTMNTGVITGKPVEWGGSLGRNEATGFGVAVVTREAAAKLGIDIKGARTAIQ
                     GFGNVGSFTVKNVERQGAKIVALGEWNREIGTFAIYNEDGLDYAELAAYQAEHKTIAN
                     FPNAKMITLDEFWALDVDIMIPAALENAITLHNADMIKAKLVVEAANGPITPEADEIL
                     EKKGILVTPDILSNAGGVTVSYFEWVQNLYGYYWSEKEVEEKEEIAMVNAFNALWKIK
                     EEFNVPMRRSAYLHSVKKVADVMKLRGWY"
     gene            complement(6477..7115)
                     /locus_tag="VFD79_02570"
     CDS             complement(6477..7115)
                     /locus_tag="VFD79_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HZH92961.1"
                     /translation="MGKDNKVSLTVIRRLPKYHRYLSDLMDKGISRISSQELSVLTGF
                     TASQIRQDLNNFGGFGQQGYGYNVEDLQRELGKILGLDQKYNTVIVGAGNLGQAIANY
                     RGFEDAGFKVLSMFDRNPRVIGLKIRDIEIRDMENIEAFIKENNVEIGIITTPKESAQ
                     EVADIYVKAGVKGIWNFAPSDLNVPDEMIIENVRLNESLFILSYFLKAATEK"
     gene            7288..>8198
                     /locus_tag="VFD79_02575"
     CDS             7288..>8198
                     /locus_tag="VFD79_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZH92962.1"
                     /translation="MPILTANHIYKTYVVDPILEDISFHVELGDKIGVLGLNGTGKTT
                     LFQILSGKLHMDQGQVQVQRGTKIGYLEQHTKIDSDLNIFDECLLVFQDLIQMEQDLR
                     EMEIEISKAGETSDDRLERIMDEYGHMLEEFTALNGYGYKSEIRGVLKGLGFVEEDFQ
                     KQVEILSGGQKSRLSLAKLLLEKPDLLLLDEPTNHLDIGAIGWLEKFLRDYRGSSLII
                     SHDRYFLDNVANRIFHMENRDLKSYNTDYTGFMVQRKKELEIIKRQYDNQQKEIKRQE
                     EIIQRFMNYGGERYIKQARSRQKMLDR"
BASE COUNT         2202 a         2029 c         1558 g         2409 t
ORIGIN      
        1 ctcctcctcg atatatagtc tttttttgtt agcactcatc tcagtcgagt gctaacatct
       61 atttataata atactctaaa acaaggagct aatcaagtcc atttttgccc taaaaacaaa
      121 ttttaacctg gaattttgaa atttatctgc ctcttgcttt agaatactat gatttacttc
      181 tattttctta aaatatcttt aaaacaaggc aactattgaa taatatacga ttggaataaa
      241 tattgccggc agcatatttg caatcttcac cttcttgatt ccaaggatat taattcctat
      301 ggccataatc agtatgccac cgatggaaga catttcattt atcacttcag gcgtcagcaa
      361 atccttcact gaactggcca agagggttat tgccccctga tataagaata ctgcaaccgc
      421 tgaaaatgca acccctattc caaaggtgga tgcaaaaatt acagctgaaa ccccatccag
      481 tacggatttt gcgtaaaggg tctcatgatt gcctgtaagg ccggattcca atgctccaag
      541 tattgccatt gcacctatgc agtagaccag ggatgccatg ataaatccct ttgaaaaatc
      601 tgagtctccc cttccgaatc ttttctccat gctggttccc agattctcaa gacgcttgtc
      661 aaggtcaagc ctctctccta tgatgctacc aaccactata ctggcaagaa caactattag
      721 gttgttcatt tcaagtgcac ccataactcc tatgataaca acagctaaag ccataccatc
      781 cattatggtg ctggtatact ggtccttgat tccctttctc agaaacactc ccaaaagtgc
      841 gcctgcaact actgctacac tgtttacaat tgttcccatc ataattatca cctaccatta
      901 ttttgtttgc tatattagat aatacagtaa aaccctaaaa ttatccagaa aaaaatgggg
      961 aatctcatat ccaattcccc atttcagcaa atctatctct ctataaagct ttctgtccag
     1021 tctggcattg gtggatttgt tccctccttg atcatttcct gccacttctc gtcagtgagc
     1081 ctttcccccc atacgaactc gtggaagctc attacagctc ccctggccag gtaaagtcta
     1141 ccatcgattg gtacaagcac atggatctca tatgccggtc cgactccagc ctccatcact
     1201 ccatcccttc cgatggtatg atagtctgaa atcactgcca tgttcttgtc tgtctcagat
     1261 gtgatttcat gccatctggt tccctgtcca gtaaaggagc ttgtcaaatc ctcaagaata
     1321 cctccatata tcttaagcct gtcatgttct tccctggtca aggggatgtc ctcaagctgc
     1381 ttgtgggatg catctattag aaagtccaga agccactgaa atctttcaag cttatggtca
     1441 atttcatcca tgttcagatc ccattcctcc aggtagtccc ttgattggtt tgtaagccac
     1501 agtagtcttt catagacctc tatgtttggc tccacataac ctggaagttc aaaatattcc
     1561 tctccaccca tctcagcacc tgacggcttt ccatacaata ctgtgtcatg cttgagctct
     1621 gcccaggatc ccagggccgt attcagattc ttgtcctccc atgcctggtt cctcatgaat
     1681 gcaggcatgt ggtcatctgc aggaccaagc agtccttcaa gagtccacat ccagccggta
     1741 taaaggtttc ttttccaatc cacctcagtg taatgggcaa attcctccct caatctttcc
     1801 atttcaacag ggtaatcctc ccactgctga ttatctggat tttcaagctg gatctccctg
     1861 gcccgctggg aacccaacac tgccattaca tcaaggcctg agggtatcgg tcgaagtatt
     1921 gggtagacca gttcttggat gatctgggag tcaaagacat acctctgtcc cataaacctg
     1981 aactgcttgt tcaccggggt attggtggag atgtacctgc ccacgatcct gggctctgga
     2041 aaactttccg ccctcttata gaagctgtca agtagatctt gactggaaaa atcctcataa
     2101 gaagcatcac ttccatatat ctcctccacc gctgcagaat aatcgggaat tcccaaatcg
     2161 tcagatgaac caacaaagaa gtttgtgggg tcatatattt tcctccattt gcccataagc
     2221 tcctcatcct ccgaaaccat cctggccatt aggattccct gcagggtctg ttcctggttc
     2281 cttacatcct cgtcaacata cagtggaaaa ggaacctgtc cataccacat caatgccagg
     2341 aagtatcttt caaggtcctc tgaccttgta taatgtcccc ttggcacata ctgactgtag
     2401 tccatctgga aaggaaaaat cacagagtca gtaaatccaa ccttggtctc tatcaattca
     2461 agctctcccg atgccatttc cagggcattc aggggaatcc ctccaggcag gtcatttttt
     2521 aacaactgct gggctacagc gaagtatgca atattctttt cctgtgccac cctcaggtcc
     2581 acatcctcca aatcctcata aatttcaatt gatttaagta gcatatcctc agtaaggtct
     2641 tcagccagtt ggagaaactt ttcattttca agagtcctta aaaggtagtc gtagaatatg
     2701 tgataagtgt gaaggactga gtcagttgtt ataaagttgg gaactttttc atactcgttc
     2761 agctcataga tgtaaaagag ctgtgcctgg tccgatggtg atacaaagaa cctgttttcc
     2821 aggagcatct ccttctgggc tggagaaagg tcaggaaaat cggacaaatt ttcaacattt
     2881 gacaggtcag tctccggcac tatatcagca ggtccctgtg gtgattcctc tggctttcca
     2941 ggattttcaa ccacttcccc tggctcatca ggaacctttt cttccaccac ttccctgcaa
     3001 cctgtaaatg caagcattag ggcaagaaag catgacagta ttacaatact tctcttccta
     3061 ttcacgatac tccctccatt ccaatccatc ttccatctct tcaacataat acctggcatt
     3121 gccagaatca tcaccaacta ttgcatggag ccggtcttca aatgtaaaaa ccttctggat
     3181 ggattttaca gcctgggtct ccttgtagcc ctcaaatcca aactctctcc acttgtaggt
     3241 attcaggacc tgccttccat cttcaaggta ctcttcccca acaacctcca tcacctggtc
     3301 attatcaata tcaaaaagat gaaagtccac aaagggtctt gaaagtcgtg aacccctcca
     3361 cttgggaacc aggtcttccc catcgaaatt gtagaagaag ggtctctttg ccattacagg
     3421 gtgaaggggc gattccttgt aaaccccaat tgaaatctca tccttgccgt ctccatccac
     3481 atctccaacc ataagcttcc atggattaag gtgtgagatg tccttcctga acacttccct
     3541 cttgccattt ccagtgtcat atatgatcag gtccttcaga tacctctggc catcctctcc
     3601 tgttagaacc accaggaagt caccctcacc ggtcaggttc cccctggcat gtaagatggc
     3661 cttgtcctgg ctacaccctg tcacaagggc cactatggcc attagaacca ctgttaagaa
     3721 gtatccccgc agcttcatcc tttcacctca cttatgtatc tgactaaagt ataacaatat
     3781 gtcactttcc ataggttacg atacagtaac aatgtgttga caaaaaagtt aagccaccgt
     3841 ctaaagacga tggcacattt cacttaccta ttcccagttc cctggcgtag tagaaaagat
     3901 attgctgggc atagcctgca aggtttccaa agattttttc tcctgactcc tcaagctccc
     3961 tcctcgtggc aggtctcttg aagtagaagt gttccatgac ccttttgacc cagacatcca
     4021 ctggaaaggc ctctggtttg tcataggcaa acaggagtat gcatccactg acctttggcc
     4081 caactcccgg gaggcttgtg agaatgtcaa ctgcatcatt gtggtcggta tcatagatgc
     4141 aaagggtgtc aaactcacct tctgcaatcc ttctgctgac ctccctgatc cggtcacccc
     4201 taaagcccac cctgtacttt tccattatat actgggtggt ttttgatgcc agcctgtctg
     4261 gctggggaaa gctgtagtag tcccttcccc tgtaggttcc gatgtagtcc ccaaggtcat
     4321 tggagattgc ctccactgca ttcttgattc ttggaatctg gttgtttgca gaaattataa
     4381 atgagattat tgtttcgtag gggtcctggt taaggagtct caatccctca ccgaaccgga
     4441 ttgcttccct gaggattgga tcccttgaaa gttccctttt taattctcca tagtccctgt
     4501 ccaggtcaaa atacttgtgc cagtattctc tccagtcctt ctcatcagtt ccatccacta
     4561 tgatctggtc tccatccttc aagatgttta tgagccttcc tccggcaact gtggtgtagc
     4621 ttccatcctc ttccttgtac cacctgaagc attgaccgtt ttcaaatata tgtgtagggt
     4681 tgaaatcctt taaaccattt attatcagct tgtcttcagc cttttctatt ttgtagtcca
     4741 tttttgtctc cttcagtgtg ttttgtatat tatactacaa aaaagatgga agtagaatct
     4801 aataagtaag aattaagaag tgagaagcaa agatgagaag cactaagttg aaagtggaag
     4861 gaaaaggagg gatccctcca tttcagtcgg gatgactgct agaatgtcat tccgagcttg
     4921 tcgaggaatc tcgcttttcg taataaacaa gaggtcaaat gaacaaggaa aagcataaaa
     4981 taaagaggca aggatttctc cttgcctcaa agttaactct atagtatttt acagcctgag
     5041 gctgattagt accatcctct aagcttcatt acatcagcaa ccttcttaac tgagtgcagg
     5101 taagctgatc ttctcattgg aacgttgaac tcttccttga tcttccaaag tgcgttgaag
     5161 gcgttaacca ttgcgatttc ttccttctct tcaacttcct tctcgctcca gtagtatccg
     5221 taaaggttct gtacccactc gaagtaagat acagttactc caccagcatt tgaaagaata
     5281 tcaggtgtaa caagtatacc cttcttctca agtatctcgt cagcctctgg tgttatagga
     5341 ccatttgctg cctcaacaac caatttagcc ttgatcatgt ctgcattgtg aagagttatt
     5401 gcattctcaa gagcagctgg aatcataatg tcaacgtcca aagcccagaa ctcatcaaga
     5461 gttatcatct ttgcattagg gaagttagct attgtcttgt gctctgcctg gtatgcagcc
     5521 agctcagcat agtcaagtcc gtcttcatta tagattgcaa atgtaccgat ttctctgttc
     5581 cactcaccaa gggcaactat tttagctcct tgtctctcaa cattcttaac agtgaagctt
     5641 ccaacatttc cgaatccttg gattgctgtt cttgctccct taatatcgat gccaagcttg
     5701 gcagccgctt ctcttgttac aacagcaact ccgaatccag tagcttcgtt tcttccaagt
     5761 gaaccgcccc actcaaccgg cttacctgta ataactcccg tgttcattgt tcctgtaagc
     5821 ttgcaatact cgtcaaccat ccatgccatt atctgaccgt tggtgttaac atctggtgca
     5881 gggatatcga ttttctcgcc taggtacttg taaaggcctt ggatatatcc tcttgacaat
     5941 ctctcaagct ctccttgtga aagagttgaa gggtcaacag ttattccacc tttacctccg
     6001 ccgtaaggga ttcctgttac acagcacttg aaggtcatcc atactgaaag tgctttaacc
     6061 tcgtcaggat tcacatctgg atgaaaacgt attccaccct tgcctggtcc aacagcgtca
     6121 ctgtgcattg atctgtagcc tttaaatacc tttactgagc cgtcatccat cttaactggg
     6181 attgaaactt caataaatct ctttggctct tttagaagtt cgtacacaga ttctccgtag
     6241 cctaatgcgt cacaagcatc ttttacctgt ttttgagcac tctccaacgg attaagtatt
     6301 tcttttgaca tggtaaaact accccctttt atttaactgt gaaaaaagct cacaataact
     6361 attaatctcg ataaaattat atcataccgc ccaaggtctg gcaaccttaa aagaaaagga
     6421 gtatttttgc cacttggatg gacggacagt tataattcaa tcgagattat caagctctac
     6481 ttttctgttg ccgccttaag aaagtatgaa agaataaaca agctctcgtt tagtcttaca
     6541 ttctcaatta tcatttcatc aggtacattc aagtcggaag gtgcaaagtt ccaaattccc
     6601 tttactccag cctttacata gatgtccgcc acttcctgtg cactttcctt tggagtggtt
     6661 atgattccaa tctccacatt gttttccttg atgaaagcct cgatgttctc catgtccctg
     6721 atttcaatat cacggatttt aagcccaatt acccttggat tcctgtcaaa cattgaaaga
     6781 accttgaatc ctgcgtcctc gaaacccctg tagttggcaa ttgcctggcc aaggtttcca
     6841 gcacccacta taacagtgtt gtacttctgg tcaagtccca gaatcttgcc aagttccctt
     6901 tgcaggtcct caacattgta accgtatccc tgctgtccga accccccaaa attattcagg
     6961 tcctgtctga tttgtgaagc agtaaatccc gtcaatacac ttagctcctg tgaggatatc
     7021 ctgctgatcc ccttatccat caggtcgcta agatatcgat gatacttagg taatcttctt
     7081 ataaccgtta atgacacttt attatctttg cccaataaat tcaccaccca tcttgaattc
     7141 tggcataatt atatcattca caaaagtata tgtcaattta ttcacaggcc tttaacaatc
     7201 tttttgcagg ttgctaaacc cctgaattta cttttaaaga agggtgtttt ctgttagaat
     7261 ggtcatagaa atcatggagg gataaaaatg ccaatactta cagcaaacca catatataaa
     7321 acctatgtcg ttgaccctat ccttgaagat ataagctttc atgttgaatt gggggacaag
     7381 ataggggttc tgggcttgaa tggaacaggg aagacaactc tctttcagat actttcaggg
     7441 aagctccata tggaccaggg acaggtacag gtccagcgag gaacaaaaat cggctacctt
     7501 gagcagcaca caaaaataga cagcgacctg aatatctttg acgagtgcct gttggttttt
     7561 caggacctta tacagatgga gcaggactta agagaaatgg agattgaaat ctcaaaggct
     7621 ggcgagactt cagatgacag gctggaaagg atcatggatg agtacggcca tatgttggag
     7681 gagtttacag cacttaacgg gtatggatac aagtctgaaa taaggggtgt ccttaaggga
     7741 cttggctttg ttgaggagga tttccaaaag caggtggaaa tcctaagcgg cggtcagaaa
     7801 tcaagattgt cccttgcaaa gctcctcctg gaaaagcctg accttcttct gttggacgag
     7861 cctaccaacc acctggatat aggtgccata ggctggttgg aaaagttcct gagggactat
     7921 aggggttcaa gcctcatcat ctcccatgac aggtactttc tggacaatgt ggccaacagg
     7981 atattccaca tggaaaacag ggatctaaaa agctacaaca cggattatac gggctttatg
     8041 gtacagagaa aaaaagagct tgagataata aagaggcagt atgacaacca gcagaaggag
     8101 atcaagcgcc aggaggagat catccaaagg ttcatgaact acgggggtga aaggtatatc
     8161 aagcaggccc gtagccggca gaaaatgttg gataggat
//
LOCUS       DASRQF010000104        10905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_892432, whole genome shotgun sequence.
ACCESSION   DASRQF010000104 DASRQF010000000
VERSION     DASRQF010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 10905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10905
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892432"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1806
                     /locus_tag="VFD79_02580"
     CDS             <1..1806
                     /locus_tag="VFD79_02580"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HZH92963.1"
                     /translation="VISGVYKKYAYIKNDDGSFIQIGERAEAVHELIGQFEFQQLINI
                     IASRGDIEYVAFIDNNYDVLASSTQDSVGSTVVDENLRIRVGNDNFSAERVEFNGQGV
                     LQTSVPIYHDEERHGVLYISWSTNVVDEEIKNLVLESLMQFLIVFIFMIGVLYYAYRK
                     NKSNIEIAYYDKLTGLPNEAYLVEYLDELLEELDEKKAIILINTRNFKALNMTYGFTF
                     GNQVIVQIAHKLRSTLGKGDMLFRFNADRFILVVNSYGTRSELVEISERIMEVFGNES
                     LVTSDNQYINIEFGVVEIPDNDITVDKLLQDATLALSHLDNTANGSIRFYEDIMEDAV
                     RREDMIEKSLRSIIISGGNDSFYLCYQPKMDVRGKKIIGYEALARLQLEDMGNVSPLE
                     FIDIAEKKQLIYDLGKLTLHKSCEFIKMTHDKGFKDISVSVNISGIQLLRKEFVDEVG
                     KIARMHEVDPRFLEFEITESVLLDNYDIINQRLRQIKDMGIVVSIDDFGTGYSSLSRL
                     REMNIDIVKIDKYFIDGIAEKDEDSLITADIISMSHKLGLKVVAEGVENKEQVEYLEK
                     HNCDIVQGYYIARPLREKDAIDKLENFKWQEHVTS"
     gene            1835..2848
                     /locus_tag="VFD79_02585"
     CDS             1835..2848
                     /locus_tag="VFD79_02585"
                     /inference="COORDINATES: protein motif:HMM:NF036772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HZH92964.1"
                     /translation="MIRYPFLGADAKIGITAPSSGVKPEMHDLLDAAILSMENKGFGI
                     VCGETPWHQIKATSAPAKVRAEEFNGMMVDDTIGLIIPPWGGELLMEVFEHIDFEGIK
                     NKWVMGYSDTSLLLLAMTLKTGIATAHGTNLIDLRGELTDDTTAKWQEVLTTRINESV
                     LQHSSQEYQREWDFENPTPHIFHLNEKTYWKSTQSGRVELKGRLLGGCVDTIRHIVGT
                     PFGDVKGFRSEHIDDEPVIWFLENCDLSTTDLRRSLVQMKLAGWFDNCNGIMFGRSDA
                     NEPVDGYTAEDIYMQLSEELNVPVVYDIDCGHVPPQITFVNGAYAEVAVEDGKGSVLQ
                     RFI"
     gene            3286..4455
                     /locus_tag="VFD79_02590"
     CDS             3286..4455
                     /locus_tag="VFD79_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HZH92965.1"
                     /translation="MKKSISLLLAMLMMLSLVVGCASPAQEPAEAPAETPAETPAEEP
                     AAAEGYKIGVMTGTVSQGEEEFRAGENMKEKYGDMIVLSTYPDRFMQEQETTITNMMA
                     MASDPDIKAIVMVQAVPGAAAAIARVKEMREDILFVLGVPGEDPDVIAEYGDVIIQTN
                     DLARGSQIAQQAKDMGAEVLVHYSFPRHMSYEMLAARRDLMKEEAEKLGLVWVEQDAP
                     DPTGDAGVPGTQQFIMEDLPRKVEEYGVNTAFFGTNCSMMEPMIRQTVETGAIFPVQC
                     CPSPYHALPAALGIAVPEDKAGDLDFILSEIDAKVDAAGMDGRVATWSVPVNMMYVEA
                     GVEYAIEYLEGNTNGKADAEVLTRIMEEVAQGEIVLGNYGNYDHYFLYLGAAVEF"
     gene            4569..6191
                     /locus_tag="VFD79_02595"
     CDS             4569..6191
                     /locus_tag="VFD79_02595"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004825081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HZH92966.1"
                     /translation="MKTEHGNEHYVLDMKDIGKEYYGTRVLSGINISLKPGEIHGILG
                     ENGAGKSTLMNILFGMSVIHNTGGFEGKVYVDGEETFIESPVEAMHKGIGMVHQEFML
                     IPGYSITENIKLNREITTPGILSRIAGKKLETLNMEEMNKDARKALDKLGMNVEEWVK
                     VAGLPVSYMQFVEIARELDKENLKVLVFDEPTAVLTESDADKLLESMRMIADSGIAIL
                     FITHRLDEVKRIADRITVLRDGQHVATKNIEDTDVFDMANLMVGREIKMDSAKSVSNE
                     DKAVMMEIKDLYVDMPGEVIKGIDLDIYEGEILGVGGLAGQGKIGLANGIMGLYNSEG
                     TVKLDGKEVVLNNPEKALKQGLAFVSEDRRGIGLLLDTSIELNIAVTAMQIQDQFINR
                     IGPFTQKDNKNIREHALKMIKDLDIRCTGPDQITRRLSGGNQQKVCIARALTLNPKVL
                     FVSEPTRGIDVGAKKLVLDTLLQLNKDYGMTIIMTSSELAELRMISHRIAIINEGKLE
                     GVLPPDASDVDFGLMMSGEYHKVKGKEVKTDG"
     gene            6184..7224
                     /locus_tag="VFD79_02600"
     CDS             6184..7224
                     /locus_tag="VFD79_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZH92967.1"
                     /translation="MDKAKVMINRFGLPRIIIVAFLLVLLVMAVMLDIPLNNLISDAL
                     VRTGMNGILVLAMVPGILAGIGLNFGIPIGIVCGLLGGLIAIELDLYGFQALFTAVLI
                     SIPLSLVAGYFYGLLLNRVKGSEMTIATYVGFSIVSLMSIGWLVLPFTSEDMRWPIGT
                     GLRTTITLQGRYERILNQWGAFDIFGVTIPTGLLLFMLFFCLLVWLFLRSKMGVAMIA
                     AGDSPKFATASGINVNKMRIVGTMLSTALSAVGIVIYSQSFGFIQLYQASMFMSFAAV
                     AAILIGGASIKKANITNVLIGALLFQSLLVVSLPVANKVLADVSSLAEIARIVISNGI
                     ILYALTQIGGGE"
     gene            7224..8516
                     /locus_tag="VFD79_02605"
     CDS             7224..8516
                     /locus_tag="VFD79_02605"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZH92968.1"
                     /translation="MDENIKITPPEMSMGQKIKKTLTHNMVTVFFIVLCIIGFYYSGL
                     TFGFLLNDIVTRMVRNSFLVLALLLPVMAGMGLNFAIVLGAMCAQAAVIFVTHWGVVG
                     LPGIGVTILLTTPLAMLLGYLTGLLFNKAKGQEMIGGMILGFFSNGIYQLIFLILLGS
                     VIPFNNETLVLSSGVGLRTTIDLAGGLKYAIDDFWRLPFTTAGMGFGALMVVHGVIRY
                     LKGIPSLGQKAGNKKYILSSVAGVALAGWLFYIAGTESFINFIKVPMLTAILIGLMCV
                     FTTYFIRTKLGQDFRTVGQDRHIADVSGIHSDKTRLTAMIMSTVFASWGHIIFLQNLG
                     TFSTYGSHEQIGFYSVAALLIGGASVTNAKISHALLGTLLFHTLFIVSPLAGRNLFGD
                     AQLGEYFRVFVAYGVIGVSLVMHAWRKQLENKKRLSQQ"
     gene            8660..9883
                     /locus_tag="VFD79_02610"
     CDS             8660..9883
                     /locus_tag="VFD79_02610"
                     /inference="COORDINATES: protein motif:HMM:NF039346.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HZH92969.1"
                     /translation="MKKIEIADGIHMLSMNVEDMLFEGIWEIANGVTLNSYIVQGEKT
                     AIIDGVIGWDGIPETLYKNLEEINVNPKDIDYLIVNHMEPDHSGWISNFEKIKKDFTI
                     ICTDKAARLVSSFYGKDKIQVVKEGDRLDLGNGKVLSFHPVPNVHWPDTMYTYENSTK
                     TLFSCDMYGSFGKMGDHNFDDEMTPEEEDFFEREGIRYYSNVMTTFTPMLRKAIEKSK
                     EFEIKTIAPGHGPLYRGNPEKIMSDYSRFSKYAEGAGKNEVTILWGSMYGMTGKAIAF
                     AEEIRKREGVKYNKLEMPLESESEMVATVFKSAGIIIAAPTYEYKLFPPVAAALDEIG
                     RKKITGKAAFRFGSYGWSGGAEKELAEILERNRMKWDMVPSVEFEGSPKAGDFEKIEA
                     GIMELIGKMKEKIVE"
     gene            complement(9973..10497)
                     /locus_tag="VFD79_02615"
     CDS             complement(9973..10497)
                     /locus_tag="VFD79_02615"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZH92970.1"
                     /translation="MSKIKLVKPTMEYSQDIMKYRDEFIKSGDSMAGCGSLRDQETVA
                     DWLHELCLLENEETCPEGKVCSNTYLAVRVEDNTLVGIIDFRHHIDHPILGLWGGHIG
                     YSVRPSQRRKGYAKKMLKQVLEKCREHGLSKVLVTCDANNVASKKTILINGGVYERDV
                     QVDGETIERYWIEL"
     gene            10717..>10905
                     /locus_tag="VFD79_02620"
     CDS             10717..>10905
                     /locus_tag="VFD79_02620"
                     /inference="COORDINATES: protein motif:HMM:NF037167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92971.1"
                     /translation="MRIVRTSFEKIQDMMEKNITRNGVTVDSFWEDYILEGSHYSIHD
                     EDSPVGYFTIHGENAITSF"
BASE COUNT         3413 a         1929 c         2633 g         2930 t
ORIGIN      
        1 gtaataagtg gagtttataa gaagtatgcc tatataaaaa atgatgatgg gtcttttatt
       61 cagattgggg agcgggcaga ggctgtgcat gagttgattg gtcagtttga gtttcagcag
      121 ctgatcaaca tcatagcaag caggggtgac attgaatatg ttgcatttat agacaacaac
      181 tatgatgtac ttgccagcag tactcaggac agtgttgggt cgacggttgt ggatgagaac
      241 cttagaataa gggtgggaaa cgacaatttc agtgctgaaa gagtggagtt taacggtcag
      301 ggtgttctac agacaagcgt tccaatatac catgatgagg aaaggcatgg tgtactctac
      361 atttcttggt ccacaaatgt ggttgatgag gaaataaaaa accttgtctt ggagagctta
      421 atgcaatttc tgatagtttt tatatttatg attggtgtac tctactatgc gtacagaaaa
      481 aacaaatcaa atattgagat tgcctactac gataagctca ctggccttcc aaacgaggcc
      541 tatcttgtgg agtatctcga tgaactactg gaggagctgg atgagaagaa ggcaataatc
      601 ctcataaaca caagaaactt caaagccctt aacatgacct atggcttcac attcggaaat
      661 caggtaatag ttcaaattgc ccataagcta aggagtactt taggaaaggg tgacatgctg
      721 ttcaggttca atgcagacag gttcatactt gttgtaaaca gctatggaac aaggtctgag
      781 cttgttgaga tatctgagag gatcatggag gtcttcggga atgaatcatt ggttaccagt
      841 gataaccagt acataaacat tgaatttgga gtagttgaaa tacctgataa tgacattacg
      901 gttgataagc tgttacagga tgccaccctg gccttgtccc acttggacaa tactgctaat
      961 ggatctatcc gtttctatga agatatcatg gaggatgctg taaggagaga ggatatgatt
     1021 gaaaaatcct taaggtctat cataataagt ggtggcaatg atagctttta cctttgctat
     1081 cagcccaaga tggatgtaag gggaaagaaa atcattggat atgaagcttt ggctaggcta
     1141 caactggaag acatgggcaa tgtttcacca ttggaattca tagatatcgc agaaaagaaa
     1201 caactgattt acgacctggg caagctgacc cttcacaagt cctgcgagtt catcaagatg
     1261 acccacgaca agggatttaa ggatattagt gtatctgtaa atatatctgg aatacagctt
     1321 ttgaggaaag aatttgtgga tgaagtggga aaaatcgcac gtatgcatga agtggatcca
     1381 agatttttgg aatttgaaat aacggaatct gtgcttctgg ataactatga tataatcaat
     1441 caaaggctaa ggcaaataaa ggatatggga atagttgtat ccatagatga ttttggcaca
     1501 ggatactctt ccctgtccag actaagggaa atgaatatcg acattgttaa gatagacaag
     1561 tattttattg atggaatagc tgaaaaggat gaggatagcc taataaccgc cgatataatt
     1621 tcaatgtccc acaagctggg acttaaggtt gtggccgaag gtgtagagaa caaggaacag
     1681 gttgagtatc tggaaaaaca taattgtgac attgttcaag gctactacat agccaggccc
     1741 ttaagagaga aggatgcaat tgataagctg gaaaacttca agtggcagga gcatgtcaca
     1801 tcatagaggt ccagaacaag ggagctgata ataaatgatc cgttatccat ttcttggagc
     1861 agatgcaaaa ataggaatta ccgcaccatc ctctggagtg aagccggaaa tgcatgacct
     1921 gttggatgct gcaatattaa gcatggagaa taagggattt ggaatagttt gtggtgagac
     1981 accatggcat cagataaaag caacttccgc acctgccaaa gttagggctg aggagttcaa
     2041 cgggatgatg gtggatgaca ccattggcct tataattcct ccctggggtg gagaactgct
     2101 tatggaagta tttgagcaca ttgactttga ggggataaaa aacaagtggg tcatgggcta
     2161 ctctgatacc agcctgctac tcttggccat gaccttgaaa actggtatag ctacggcaca
     2221 tggaacaaac ctgattgact tgagaggaga gcttacagat gatacaactg caaagtggca
     2281 ggaagtattg actaccagaa tcaatgagtc agtgcttcag cattcgtctc aagagtatca
     2341 gcgggaatgg gattttgaaa atccaactcc ccacatattc cacttgaatg aaaagactta
     2401 ctggaagtca acccaaagtg ggagggtcga attgaaaggt agactcttgg gcgggtgcgt
     2461 cgatacaata agacatattg taggaactcc ttttggtgat gttaaaggct ttagaagtga
     2521 acatatagat gatgaaccgg taatctggtt tcttgagaac tgtgatttat ccaccaccga
     2581 tttaaggaga tcacttgtac agatgaagct tgcaggatgg tttgataatt gtaatggaat
     2641 aatgtttggc aggagtgatg ccaatgaacc ggtggatggc tacactgctg aggatatcta
     2701 catgcagctt tcggaagagc ttaatgttcc tgttgtgtat gatattgact gtggacacgt
     2761 ccctccccag atcacgtttg ttaacggtgc atatgcagag gtagcagtgg aggatggcaa
     2821 gggttcggta ttacagagat tcatctagga aactgagatg ggcatcattg taaacaagga
     2881 tctgagagta ggtttcaaat gagagaatat gatgattctg ttgataggct actggtattt
     2941 ggtttacaat aactgcagag aataatatga tacccttgat ggattaatcg atttatgcac
     3001 atttaaaaac tcctgcaagg cctgcttaga agaggctttc aggagtttta cacattcttg
     3061 aagtcaattg gactcaatat ttaataatca acatatgaca attaacaaac aaagacataa
     3121 taattagaaa attcttgctt tattctcaac tcttgagtat aatctttata agtgttaaat
     3181 caggtaggaa aaatgcctga gtattaatct tgaggggagg agtgccagaa cggagtagta
     3241 tcagcatttc actaaaatca aaacgaaaat ttaaggggga ttcaaatgaa aaaaagtata
     3301 tcattattac ttgcaatgct tatgatgtta agtttggtag ttggatgtgc atcacctgct
     3361 caagaaccag cagaggcacc tgcagaaact ccagcagaaa ctccagctga agaaccagca
     3421 gcggcagaag gctacaagat tggtgttatg actggaactg tatctcaagg tgaagaagag
     3481 tttagagccg gagagaacat gaaggaaaaa tatggtgaca tgatagttct ttctacatat
     3541 ccagacagat ttatgcagga gcaggaaact acaattacaa acatgatggc aatggcttct
     3601 gaccctgaca taaaagcaat cgtaatggtt caggctgtac caggtgcggc agctgctatt
     3661 gcaagagtaa aagaaatgag agaagacata ctatttgttc tcggagttcc tggagaggac
     3721 ccagatgtaa ttgctgagta cggtgatgta ataattcaaa caaacgacct tgctcgtgga
     3781 tcacagatag ctcaacaagc aaaggacatg ggagctgaag tacttgttca ctactcattc
     3841 ccaagacata tgtcatatga aatgcttgca gcaagaagag accttatgaa ggaagaagct
     3901 gagaagcttg gtcttgtatg ggttgagcag gatgcaccag acccaactgg agatgctgga
     3961 gttccaggaa cacagcaatt cataatggaa gaccttccaa gaaaggttga agaatatgga
     4021 gttaatactg cattctttgg aacaaactgt tcaatgatgg agccaatgat cagacagaca
     4081 gtagagactg gagcaatctt cccagttcag tgttgtcctt ctccttacca tgcattacca
     4141 gcagctcttg gtatagcggt accagaggac aaggcaggag atcttgactt tatcctaagc
     4201 gaaatcgatg caaaagttga tgcagctgga atggatggaa gagtagcaac ttggtcagtg
     4261 cctgtaaaca tgatgtatgt tgaggctgga gtagagtatg ccatagagta ccttgaagga
     4321 aatacaaacg gcaaggctga cgcagaggtt cttacaagaa tcatggaaga agtagctcag
     4381 ggagaaatcg tacttggaaa ctatggaaac tacgaccact acttcctata tcttggagca
     4441 gctgttgaat tttaataatt taaatcgata aaaaacaaaa tgatgtagaa aacaatattg
     4501 tgcaaggctt ttgccttgca caatattatg tcactctctg aaatttgttt aaaggaggga
     4561 acaatcgttt gaagacagaa catggtaatg agcattatgt cctggacatg aaggacatag
     4621 gcaaggagta ctacggaaca agggtattgt caggaatcaa cataagcctc aagccaggag
     4681 aaatacatgg gattcttggg gaaaacggtg caggaaaatc aacccttatg aatatactgt
     4741 tcggtatgtc tgtaatacat aacacaggag gatttgaagg aaaggtctac gtggatggag
     4801 aagagacctt tattgaatct ccggttgagg caatgcataa gggaatagga atggttcacc
     4861 aggagttcat gctgattcct ggttatagca taactgagaa cataaagctt aacagggaaa
     4921 tcacaactcc tggaatccta agcaggattg ctgggaagaa gctggaaacc cttaatatgg
     4981 aagagatgaa caaggatgcc cgcaaggcat tggacaaact aggcatgaac gtggaggagt
     5041 gggtcaaggt tgcaggtcta cctgtaagct acatgcaatt tgttgagatc gcaagggagc
     5101 ttgacaagga gaacctgaag gttttggtgt ttgatgagcc aacggccgtg cttacagaaa
     5161 gtgatgccga caagcttctg gagtccatga gaatgattgc agactctgga attgccatac
     5221 tattcataac acacagactt gatgaagtta agcgtatcgc tgatagaatt actgttttgc
     5281 gtgatgggca acatgttgca accaagaaca tagaggatac agacgtgttc gacatggcga
     5341 acctgatggt tggccgtgag ataaaaatgg actcagccaa gtccgtatca aatgaggaca
     5401 aggcagtaat gatggaaatc aaggaccttt atgttgatat gccaggtgag gtcataaagg
     5461 gaatcgacct tgatatatat gaaggtgaga ttcttggagt tggcggcctt gcaggccaag
     5521 gtaaaattgg tcttgcaaac ggaataatgg gtttatataa ttctgaagga acagttaagt
     5581 tggatggaaa agaagttgtc ctaaacaacc ctgagaaggc tctgaaacaa ggattagcat
     5641 ttgtttccga ggataggagg gggataggac ttttacttga tacatcaatt gaattgaata
     5701 ttgctgtaac tgcaatgcag atacaggatc aattcataaa tagaattggt ccctttacac
     5761 aaaaggacaa caagaatatc agggaacatg ctcttaagat gatcaaggac ctggacatac
     5821 ggtgtaccgg gcctgaccag ataacaagaa ggttaagtgg aggaaaccag cagaaggtat
     5881 gtattgcaag ggctctcact ctgaatccaa aggtcctttt cgtatcagag ccaacaaggg
     5941 gtattgatgt tggtgctaaa aaactggttc tggatacctt gctgcagctt aacaaggact
     6001 atggtatgac gataataatg acatctagtg aacttgcaga gcttaggatg atatcccaca
     6061 ggatagccat aatcaatgaa ggcaagcttg aaggagtcct acctccggat gcaagtgatg
     6121 ttgactttgg tttgatgatg tctggtgaat accataaggt caaaggtaaa gaggtgaaga
     6181 cagatggata aggcaaaggt aatgatcaat cggtttgggc ttcctaggat aataatcgtt
     6241 gcattccttc tagtgttgct tgtaatggca gtaatgcttg acataccgct gaacaacctt
     6301 atttcggacg cattggtacg tacgggaatg aacgggattc ttgtccttgc catggttccg
     6361 ggtatattgg cgggaattgg tctgaacttc gggattccaa ttggtatagt atgtggacta
     6421 ttgggtggac tgattgcaat agagctggac ctgtatggtt tccaagccct gtttacggct
     6481 gttcttataa gtattcccct atcacttgtg gcaggatatt tctacggtct cttgcttaac
     6541 agggttaagg gatctgaaat gacaatcgct acatacgtgg gattctcaat agtatccctt
     6601 atgagtatag gttggctggt acttccattt accagtgaag acatgagatg gccaataggc
     6661 acaggattaa ggacaacaat caccctacag ggaagatacg aaagaatcct gaaccaatgg
     6721 ggggctttcg atatatttgg agttacaatc ccaactggac ttctcttgtt catgctcttc
     6781 ttctgtttac ttgtttggct tttcttaagg tcaaaaatgg gtgtcgccat gatagccgca
     6841 ggagatagcc ccaagtttgc cacagcatca ggaataaatg taaacaagat gcgaattgtt
     6901 ggaacaatgc tttcaacggc attaagtgct gttggtatag taatctacag tcagagcttt
     6961 ggatttattc agctttacca agcttcaatg tttatgagct ttgcagccgt ggcagcaatt
     7021 cttataggtg gtgcatccat taaaaaggca aacattacga atgtacttat cggagctctt
     7081 ctgttccagt cattgcttgt ggtgtcccta ccagttgcca acaaggtact ggcggatgtt
     7141 agcagtttgg cggaaattgc tcgtattgta ataagtaacg gcataattct ttatgcattg
     7201 acacagattg gaggtggaga ataatggatg aaaacatcaa aataacccca cctgaaatga
     7261 gtatgggaca aaagatcaag aagaccttaa cccacaatat ggttacagta ttctttattg
     7321 ttctctgtat aataggattc tactattcag gattgacttt cggttttctt cttaacgata
     7381 tcgttacacg tatggtaagg aacagcttcc tggtcttggc cttgcttttg ccggttatgg
     7441 ctggaatggg ccttaacttt gcaattgttc ttggggccat gtgtgcccag gcggcagtta
     7501 tatttgttac ccactggggt gtagttggac tccctgggat tggcgtgacc atattattga
     7561 caactccact ggcaatgctt ttgggatacc tgacaggact tttgttcaac aaggcaaagg
     7621 gacaggaaat gattggtgga atgattctgg gattcttttc aaatggaatt taccagttga
     7681 tattccttat tctgctggga tcggtaatcc cattcaacaa tgaaaccctt gtacttagct
     7741 caggagttgg acttaggacc acaattgacc ttgcaggtgg attgaaatat gctattgatg
     7801 acttctggag acttccattc acaacagcag gaatgggttt tggagcactg atggttgtgc
     7861 atggagtcat aaggtatctt aagggaatac cttcccttgg tcaaaaggct gggaacaaga
     7921 agtatatcct ttcctccgtc gcaggcgttg cacttgcagg ttggctattt tacatagcgg
     7981 gaactgaatc ctttataaac tttataaagg tgccaatgct aacagccata ctgatcggcc
     8041 tgatgtgtgt tttcaccacc tattttatca ggacaaagct gggtcaggac ttcagaactg
     8101 tgggccagga caggcacatt gcggatgtat ctggaatcca ctcagacaag acaaggctta
     8161 cagcaatgat aatgtcaacg gtatttgcat cctggggaca tatcatattt ctgcagaacc
     8221 ttggaacctt cagtacctat ggtagtcacg agcagatagg cttctattcc gtagcggcat
     8281 tgttgatcgg tggcgcctca gttacaaatg ccaagatcag tcatgcacta ctgggtaccc
     8341 tgctattcca tacactattt atagtgtcac cacttgcagg tagaaacctc tttggcgatg
     8401 cacagcttgg agagtacttc cgtgtatttg tagcctatgg agtcattgga gtttcactgg
     8461 taatgcatgc atggagaaag cagcttgaaa ataagaaaag attaagtcag cagtaatata
     8521 taatggttaa gttaaaaagg cttcaggata atcctggagc cttttatagt ggcttgacta
     8581 aaaaaacaca caggatataa ttaacttgaa ggtaaaatgg gtaaaaaggt tctataaatt
     8641 ggatttaagg aggataaaga tgaaaaaaat cgaaatagca gacgggatac atatgttatc
     8701 catgaatgtt gaagacatgc tttttgaagg aatctgggaa attgcaaacg gagttaccct
     8761 aaattcatat attgtacagg gtgagaaaac agcaataatt gatggtgtga ttggatggga
     8821 cggaatacct gaaaccctgt ataagaatct tgaagagatt aatgtcaatc caaaggatat
     8881 agattatctt atagtaaacc acatggagcc agaccattca ggctggattt ccaactttga
     8941 aaagatcaag aaggacttta ccattatctg tactgacaag gctgcaaggc tagtgtcatc
     9001 attttatgga aaggacaaga tccaggttgt aaaagaaggg gatagactgg accttggaaa
     9061 tggaaaggta ctgagctttc accccgttcc aaacgttcat tggccagata caatgtacac
     9121 ctatgaaaac agcacaaaga ccctattctc atgtgacatg tacggatctt tcggaaagat
     9181 gggcgaccat aatttcgacg atgaaatgac accggaggaa gaggatttct ttgagaggga
     9241 gggaatcaga tactactcca atgtaatgac taccttcact ccaatgctca gaaaggcaat
     9301 agagaaatca aaggaatttg aaataaagac aatcgcacca gggcatggtc ctctatatag
     9361 aggaaatccc gagaagatta tgagtgacta ctccaggttc tccaaatatg cagagggtgc
     9421 aggaaagaac gaggtaacta tactatgggg ctccatgtac ggtatgactg gcaaggccat
     9481 agcctttgca gaggagattc ggaagaggga gggagtaaag tacaataagc tggaaatgcc
     9541 acttgaaagc gaaagtgaga tggtcgctac ggtgtttaag tcagcaggaa taataattgc
     9601 tgcaccaacc tatgagtaca agttattccc acctgtggca gctgcacttg atgagattgg
     9661 cagaaagaaa attacaggaa aggcagcctt cagattcgga tcctacggat ggtctggagg
     9721 agctgagaag gagctggctg aaatacttga gagaaacaga atgaagtggg atatggtccc
     9781 ttcagtggaa tttgagggct cccccaaggc aggtgacttt gagaagattg aagctggaat
     9841 catggagctt attggcaaga tgaaggagaa gatagtggaa taggaatttt ggctggtccc
     9901 cattggggac cagctatttt ttgacgaaaa gtacacctac tgatggaagt tgataatttt
     9961 caaaaatgga agctacaact caatccaata gcgttctatt gtttcaccat ccacctggac
    10021 atccctttca tagacaccac cgtttatcag gatagtcttt ttgcttgcaa cattgtttgc
    10081 atcacaggtt accaaaacct tgcttaagcc gtgttctctg cacttttcaa gaacctgctt
    10141 aagcattttc tttgcatagc cctttctccg ttgggagggt cgtactgaat agccaatgtg
    10201 gcctccccaa agtcctagga ttgggtgatc aatatggtgt ctgaagtcga tgataccaac
    10261 aagtgtgttg tcctcgaccc tgacggctaa atatgtattt gaacacacct ttccttcagg
    10321 acatgtttct tcattttcaa gcaggcaaag ctcatgtagc cagtctgcta cagtttcctg
    10381 gtccctcagg ctcccgcacc ctgccatgct gtctccagac ttgatgaact catccctgta
    10441 tttcataata tcctgggaat actccattgt cggtttcact agttttatct tggacatgtt
    10501 atcaccatct ttctaatatt tgcggaaata agtcatttgt ttactagata atactataat
    10561 gaacctcacc atacaattaa aatatccaca aatattaaaa aatacacaca tccaacaaat
    10621 aaatcactaa atactcactg atttgttgta taatcaaggt gacatattat cgtgcagaat
    10681 caagcccata ttttaaataa ttaccgggag ggaaacatgc gaatagtaag aacaagcttc
    10741 gagaaaatcc aggatatgat ggaaaaaaac ataacaagga atggagtaac agttgattca
    10801 ttctgggaag attatatcct ggaggggagt cattactcca tacatgatga agattcaccc
    10861 gttggatatt ttacaattca tggagaaaac gcaataactt ccttc
//
LOCUS       DASRQF010000105         7767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1710365, whole genome shotgun sequence.
ACCESSION   DASRQF010000105 DASRQF010000000
VERSION     DASRQF010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 7767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7767
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1710365"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="VFD79_02625"
     CDS             <1..871
                     /locus_tag="VFD79_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZH92972.1"
                     /translation="MIAHIEVSIDFPEDDIEEVTYEDLIRDGNLVRDRIGKLLATSGR
                     GKILRDGLNTVILGKPNVGKSSLLNAVLRENRAIVTDVPGTTRDIIEEYVNIDGIPLR
                     IMDTAGIRHTEDLVEKIGVDKAKETLEKADLIIAIFDISRSLSEDDHHIIDLIKDKKT
                     IVLLNKTDLPREYDEALFREYFDANQIISTSMIKGVGIDELENAIRAMFYGGDIQMDS
                     EMIITNIRHKNQLEKAMVNIGSVLDDLAHEIPLDCLEVDLRSCWENLGEITGESITED
                     ILDKIFSEFCIGK"
     gene            895..2802
                     /gene="mnmG"
                     /locus_tag="VFD79_02630"
     CDS             895..2802
                     /gene="mnmG"
                     /locus_tag="VFD79_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HZH92973.1"
                     /translation="MIETKTYLGGKYDVVVVGAGHAGVEAALAASRMGLQTLILTISL
                     DGIVALSCNPNIGGTGKGHLVREIDALGGEMALNIDKSFIQSRMLNLSKGPAVHSLRV
                     QADKRVYHTEMKKILEDEPNLTLRQGEAIDILFDEEAIKGVVTRTGAVYETQAVIIAT
                     GTYLGGRVYIGEVNYQSGPDGMFPSLLLSDSLKEKGFQLRRMKTGTPARVHRDSVDYS
                     VMEIQPGDEEVIPFSFLNMGKTFDFEQVPCYLTYTTEDTHEIIRQNLDRSPMFSGYIE
                     GVGPRYCPSIEDKVVRFGDRDKHQVFIEPEGLSTKEMYVQGVSSSLPEEVQINMYRKI
                     KGLENVQFMRSAYAIEYDSINPTMLKRSLEHKQIRNLFFAGQINGSSGYEEAAAQGLI
                     AGINAAQNIKGGEPFILDRSEAYIGVLIDDLVTKGTEEPYRMMTSRAEYRLTLRQDNA
                     DLRLTRKGYDLGLVTEERYRKTVEKEELIENELKRLRKLIINPNSDNNRTLEGIGSTA
                     IKNATSLYDLIKRPEVSYDSTLVFDPEREPLIKEIRLQVETQIKYEGYIKKQMIQIEQ
                     FKKLENKKLSVEFDYSRVKGLSNEALQKLTAIKPDSVGQASRISGVSPADINVLLIHL
                     EQRRRNGEKDE"
     gene            2795..3511
                     /gene="rsmG"
                     /locus_tag="VFD79_02635"
     CDS             2795..3511
                     /gene="rsmG"
                     /locus_tag="VFD79_02635"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HZH92974.1"
                     /translation="MNDDRLKRLSTSMGLELTDEMLHDFTVYKEMLKEWNEKINITSI
                     TEDEEIDKKHFVDSLSPALTGIFDGDKEIIDIGTGGGFPGLPLKIRNRNLKVTLLDSL
                     NKRIIFLDQVINKLGLENIVAVHGRAEEMGLKPDYREKYDVCVSRAVASLDTLSEYCM
                     PFVKVGGYFISMKGPEVDDEVKESTKAIETLGGQVEEIKHVQIPESDIVHSLVVIRKI
                     RNTPKKYPRAGGKPKKSPIK"
     gene            3730..4494
                     /locus_tag="VFD79_02640"
     CDS             3730..4494
                     /locus_tag="VFD79_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZH92975.1"
                     /translation="MGKTMAVFNQKGGVGKTTTSINLAAGLGKLGKRVLLVDIDPQGN
                     CSSGIGIDRDEAQILVYDILTGEADIKEGIYETNAENVSIVPSNNQLAGLEIELARGG
                     DWEESLKMIINQVRDEYEYILIDCPPSLGILSVMGLIAADSVIIPIQCEYYALEGVSQ
                     LFQTIELVKKNYNPTLEVEGVVLSMFDGRTNLSIQVVEEVKSFFKGKVYTSIIPRNVR
                     LAEAPSYGLSIHDYDSRSKGSESYMDLAEEFLYLQD"
     gene            4525..5385
                     /locus_tag="VFD79_02645"
     CDS             4525..5385
                     /locus_tag="VFD79_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HZH92976.1"
                     /translation="MTSKKRGLGKGLSALIQDKEKVDTLIGDSSNRDETVVSLPLDGI
                     IPKPNQPRKIFQEEALDDLAQSIRENGIIQPIIVRKLEKGHEIIAGERRWRAAKEAGL
                     EEIPCIIREIDDVTADRISLIENIQRENLNPIEEAQAYKRIMKEYSLKQDELAKAVGK
                     SRSYISNNMRLLNLDDRVVQYIYEGKISGGHGKALLGIKNKEDQVKAAEKIVQFGLNV
                     RDTESTVKKKKKVRSKKTKENYILDLEEQLMSALGTKVTLNTGRKSGKIEIEYYNDDD
                     LERLIDLITS"
     gene            5418..6311
                     /locus_tag="VFD79_02650"
     CDS             5418..6311
                     /locus_tag="VFD79_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HZH92977.1"
                     /translation="MEVNIFQVDAFTDKPFGGNPAGVVTNSKGISSQQMQKIANEMNL
                     SETAFVRQLDDCYFKIRYFTPLCEVDLCGHATIAAIHTLANKGYIKPIEKGGKIANIE
                     TGVGTLKVEIQFENYSPVSIAMEQDTPQSMGPLENLSELLECFGLEVDEVGIGQGEAS
                     PEIVSTGLPDIILPVRTRRRLMDMKVDFCRLRDLSNKLGVTGVHAFYYDPSQEMAHAR
                     NFGPAVGIDEEAATGTANGALIYYLRKNGYLEGDSLLVHQGESLGRPSMINCYIEEKG
                     GKYSVKVGGSAVIILEGIIKF"
     gene            6331..6972
                     /locus_tag="VFD79_02655"
     CDS             6331..6972
                     /locus_tag="VFD79_02655"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HZH92978.1"
                     /translation="MLIERTAKDFVYDVASESPTPGGGSVAALVGSLGGALTNMVGNL
                     TIDKKIYADVPEDKKATMEKNFKELEVLVDELLDLVDEDSTAFDDVMQAFKLPKDTDG
                     NKKIRSEAIQSGYKKALEVPLRCGERCHDVLKLQNIFAEHGNINAITDVGVGTLLAYA
                     GLEGALFNVTINLKSIKDQDYKNEMEKRVETLLMDGKALRDSLLEVVYGRLKA"
     gene            7127..>7767
                     /locus_tag="VFD79_02660"
     CDS             7127..>7767
                     /locus_tag="VFD79_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZH92979.1"
                     /translation="MYYFDNGATTFPKPQEVYDEIYKAMTEYGANPGRSGHDLALRAS
                     RGIYETREGLARLFSIYNPMNIVFTYNCTESLNIAIKGLLKPGDHVITTSMEHNSVLR
                     PIKHLETIGVQNTIVWAHKDGQIDPAAVRDAIRKNTRLIVTTHVSNLTGTIMPVKEIG
                     MIAREHGIPYLLDAAQSAGVYQIDVEDLGVDMLAFPGHKGLLGPQGTGGLYIR"
BASE COUNT         2511 a         1408 c         2001 g         1847 t
ORIGIN      
        1 catgattgcc catattgagg tttccataga ttttcctgag gatgatatcg aagaggttac
       61 ctacgaggac ctgataaggg atggaaatct ggttagggat aggattggaa aactccttgc
      121 aacctcgggt aggggcaaga tactcaggga tggcttgaat accgttatcc ttggaaagcc
      181 aaatgttgga aagtcctccc ttctcaatgc tgtgttgagg gagaacaggg caattgtcac
      241 ggatgttcca ggtaccacca gggatataat tgaagagtat gtcaacatcg atggcatacc
      301 tcttaggatc atggatacag cgggtataag acatactgag gacctggttg aaaaaatagg
      361 tgtggacaag gctaaggaaa ccttggaaaa ggctgacttg ataattgcaa tctttgacat
      421 ctcaagaagt ctctctgagg atgatcacca tattatagac cttattaagg ataagaagac
      481 catagtactc ttgaacaaga cagatcttcc aagggaatat gacgaggcgc ttttcaggga
      541 atattttgat gcaaatcaga taatcagcac atccatgata aagggtgtcg gaattgatga
      601 gctcgagaat gccattagag ccatgtttta cggtggagac atacaaatgg attccgaaat
      661 gataataacc aatatacggc acaagaacca gctagaaaag gcaatggtaa atattggaag
      721 tgtattggat gacctggccc atgagattcc actggattgt ctagaggtgg atttaaggag
      781 ctgctgggaa aatctgggag aaattaccgg cgaaagtata actgaggata ttcttgataa
      841 gatattcagt gagttctgca taggcaagta gacaaacaga gaaaggtgag cattatgatt
      901 gaaacaaaaa cctatttagg tggcaaatat gatgtagtag ttgtaggtgc gggacacgca
      961 ggtgttgagg cagctcttgc agccagcaga atggggctgc agaccctgat cctgaccatc
     1021 agtcttgatg gcattgtcgc cctgtcctgc aatcccaata tcggaggtac tggaaagggg
     1081 catttggtac gtgaaattga cgcccttggg ggagaaatgg ctttaaatat tgacaagtca
     1141 tttattcaga gtagaatgct caacctctca aagggaccgg cagtccattc ccttagggtg
     1201 caggctgaca agagggtcta ccatacagaa atgaagaaga ttctggaaga tgaaccaaac
     1261 ctgacattgc gacagggtga agcaatagac atactgtttg atgaggaagc cattaagggt
     1321 gttgtaacca ggacaggtgc tgtgtatgag acccaggcgg ttataatagc aacgggaacc
     1381 tacctgggag gcagggtcta cattggagag gtaaactacc aatcaggacc tgatggaatg
     1441 ttcccctccc tcctcctatc cgattcccta aaggaaaagg ggtttcagtt gagaaggatg
     1501 aaaacaggca cccctgcaag ggtgcaccgg gacagtgttg attactccgt tatggagatt
     1561 caaccaggcg atgaggaggt tattccattc tcattcctca atatgggaaa gacctttgat
     1621 tttgaacagg tcccctgcta ccttacctac acaacagagg acactcatga aataattagg
     1681 caaaaccttg accgatcacc aatgttttca ggttatatag aaggtgtggg tccaaggtat
     1741 tgtccatcca tagaggataa ggttgtaagg tttggagata gggacaagca ccaggtcttc
     1801 attgaaccag agggcttgtc aaccaaggag atgtatgtgc agggtgtcag ctcttccctg
     1861 cctgaagaag tccagataaa catgtacagg aagataaagg gccttgagaa tgtacaattc
     1921 atgagatctg cctatgcaat tgagtatgat tcaataaacc cgacaatgct taagaggagc
     1981 cttgaacaca agcagataag gaatcttttc ttcgccggac agatcaacgg gtcttcagga
     2041 tatgaggaag ctgcagccca ggggcttatt gcaggcatta atgcagcaca aaacataaag
     2101 ggcggggaac cttttatcct ggataggtca gaggcctaca ttggggtatt gatagatgac
     2161 ctggtaacaa agggaaccga agaaccctat aggatgatga cttcaagggc tgaatacagg
     2221 cttactcttc gacaggacaa tgctgacctg aggctgacca ggaagggtta tgacctgggt
     2281 cttgtcactg aagaacgcta tagaaagacg gttgaaaagg aagaattgat agaaaatgag
     2341 cttaagagac tcagaaagct aattattaat cccaattcag acaataatag aactcttgaa
     2401 ggtattggat ccactgcaat caagaatgcc acatccctgt acgaccttat caagagaccg
     2461 gaggtttcct atgacagcac acttgttttc gatcctgagc gggaaccgct catcaaggag
     2521 attaggcttc aggttgagac ccagatcaag tatgaaggat acatcaagaa gcagatgatt
     2581 caaattgaac agtttaagaa acttgagaac aagaagcttt cggtggagtt tgactattca
     2641 agggttaagg gattgagtaa cgaggccctt cagaagctta ctgccataaa accggattca
     2701 gtgggtcagg cctcaagaat atcgggtgtc tcaccagctg atataaacgt cctgctgata
     2761 catcttgaac aaaggcgaag gaatggtgaa aaggatgaat gatgacagac ttaagagact
     2821 ttccacttca atgggccttg aactgactga cgagatgctt catgatttca cagtctacaa
     2881 ggaaatgcta aaggaatgga atgagaagat caatataaca agtatcacag aggacgagga
     2941 aattgataag aaacattttg tggacagcct ctcccccgca ctgaccggga tatttgatgg
     3001 agataaggaa ataatcgata tcggaaccgg tggaggcttc cctggacttc ctttgaagat
     3061 aagaaacaga aatctgaagg taaccctact ggacagcctc aacaagagaa tcatattttt
     3121 ggatcaggtt atcaacaagc ttggacttga gaacattgtt gctgttcacg gaagagctga
     3181 agagatgggg ctaaaacctg actacaggga aaagtatgat gtttgcgtgt ccagggcagt
     3241 ggcatcactg gataccttaa gtgagtattg tatgcccttt gtaaaggttg gaggttactt
     3301 catatcaatg aaggggcctg aggtggatga tgaggtcaag gagagcacta aagccattga
     3361 aacactgggt ggccaggtgg aagaaatcaa acatgtacag attcctgaaa gcgacatagt
     3421 ccattcatta gtggttataa ggaagattag aaacactccc aagaagtacc ctcgtgcagg
     3481 aggaaaacca aagaaatcac ctatcaagta aaggtaagca ggaaatataa gtggaaatat
     3541 aatttcagaa atccatagaa ccgttgaaaa caaacagata cagggaaatg ttccacgtga
     3601 aacatcaaaa atgttccacg tggaacattt ttgtctataa ttcaatatta tatgttatta
     3661 tatatctaag gaataatttt ggtaggttta aagagtgatt aatgaacata cggattgggg
     3721 agtgtgtata tgggaaaaac catggctgtt ttcaatcaaa aaggtggtgt gggaaagaca
     3781 accaccagca tcaacttggc agcaggtctt ggcaagctgg gcaagagggt gttacttgtg
     3841 gacatagacc cccagggcaa ctgttcctca ggtataggca tagacaggga tgaagcccaa
     3901 atactggtgt atgatatcct tactggtgaa gcggacataa aagagggtat atatgaaacc
     3961 aatgctgaaa atgtgagcat tgttccctcc aacaaccaat tggctggcct tgaaatcgag
     4021 cttgcacggg gaggagattg ggaagagtcc ctaaagatga tcataaacca agtaagagat
     4081 gagtacgagt acatcttaat agactgccct ccctcacttg gaatattgag tgtaatgggc
     4141 cttattgctg cagatagcgt aattattccc atacaatgtg aatactacgc ccttgagggt
     4201 gtaagccagc tgttccagac aattgagctg gtcaagaaaa actacaaccc gaccctggaa
     4261 gtggaaggtg ttgtactgag catgtttgat ggaagaacaa atctttccat ccaggttgtt
     4321 gaggaggtta agtccttctt caagggtaag gtttacacaa gtataattcc cagaaatgta
     4381 agactggctg aggcgccgtc ttatgggctg tcaatacacg attacgattc aaggtcaaag
     4441 ggttcggagt catacatgga tttggccgaa gaatttctat atttgcagga ttagtctatt
     4501 cagttcagaa aggatgtgtt gctattgacc agcaagaaaa gaggtcttgg caagggatta
     4561 tcagcattga tccaggataa ggaaaaggtg gataccctta ttggggattc ctccaacagg
     4621 gatgagacgg ttgtatccct gcccctagat ggtataattc caaagccaaa ccagcccaga
     4681 aagatctttc aggaggaggc attggatgac cttgcccaat ccattaggga aaatggaatt
     4741 atccagccta ttattgttag aaaactggag aaaggccacg aaatcatagc aggtgaaaga
     4801 agatggaggg cggccaagga agcaggcctt gaagagattc catgcattat cagagagata
     4861 gatgacgtta cagctgaccg gatttcgctt atagaaaaca tccaaaggga gaaccttaat
     4921 ccaatagagg aagcccaggc atataaaagg ataatgaagg agtacagcct aaagcaggat
     4981 gagttggcaa aggctgtggg aaagagcagg tcatatataa gcaacaatat gaggcttctg
     5041 aatttggatg acagggtagt ccagtatata tatgaaggaa agatatccgg tggacatggg
     5101 aaggcattac ttggaatcaa gaacaaggaa gatcaggtaa aggctgcaga gaagattgtt
     5161 caatttggac ttaacgtgag agatacggaa agcacagtta aaaagaagaa gaaagtaaga
     5221 agtaaaaaaa ctaaggaaaa ctatatactt gacctggaag agcagcttat gagtgccttg
     5281 ggaacaaagg tcaccttgaa cacaggtaga aagtcaggaa agattgaaat tgagtactac
     5341 aatgatgatg acttggagcg gcttattgac ttgattacaa gctaatgttc cacgtggaac
     5401 acaaaggagg ttattcaatg gaagtaaaca tatttcaggt tgatgcattt acagacaaac
     5461 cattcggtgg gaatcctgca ggcgttgtca caaactccaa ggggatttcg tcccagcaga
     5521 tgcaaaaaat agcaaatgag atgaaccttt cagaaacagc cttcgttaga cagcttgatg
     5581 attgctactt caagatacga tatttcactc cactctgcga ggttgaccta tgtggccatg
     5641 caacaatagc tgcaatccat acacttgcaa acaaaggtta tataaaacca attgagaagg
     5701 gtggaaagat agccaacatt gaaactggag taggtacctt aaaggttgaa atccagtttg
     5761 agaactactc tccagttagt attgccatgg agcaggacac accccagtcc atgggaccgc
     5821 tggagaacct cagtgagctg ttggaatgct ttggattaga ggttgatgaa gttgggatag
     5881 gccaaggtga agcctcacca gagatagtat ccacagggtt gcctgatata atcctaccgg
     5941 tcaggaccag gaggagactt atggatatga aggttgactt ctgtagattg agagatctta
     6001 gcaacaagct tggagtgaca ggtgtccatg cattttacta tgacccctca caggaaatgg
     6061 cacatgcaag aaattttgga ccagcagtgg gtatagatga ggaagcggct actggtacag
     6121 ccaacggagc actgatatac tatcttagga agaatgggta cctggaggga gacagtcttc
     6181 ttgtccatca gggggagtcc cttgggagac ccagtatgat caactgttat attgaagaaa
     6241 aagggggtaa atactcagtc aaagtaggag gatctgcagt aataatattg gaaggaataa
     6301 ttaaatttta aataactatg gaggtgcaac atgttaattg aaagaacagc aaaggatttt
     6361 gtatacgatg tagccagtga gagtcccacg ccaggtggcg gaagcgtggc agccctagtg
     6421 ggcagccttg gaggagccct tacaaacatg gtgggaaacc tgaccattga caagaaaatc
     6481 tacgccgatg taccagagga caagaaggca acaatggaaa agaacttcaa ggagctggaa
     6541 gtccttgtag atgagctttt ggaccttgta gatgaggatt ccacagcatt tgatgatgta
     6601 atgcaggcat tcaagctgcc aaaggacact gatgggaaca agaagataag gtcagaggca
     6661 atacagtcag gctataagaa ggctcttgag gttcccttga gatgtggaga aagatgccat
     6721 gatgtattga agcttcaaaa tatttttgca gagcatggaa acatcaatgc cattacagat
     6781 gttggagttg gaacactttt ggcatatgct gggcttgaag gagccctatt caatgtgaca
     6841 atcaacctta agagcatcaa ggatcaggat tataagaacg agatggaaaa aagggttgag
     6901 actctgttaa tggatgggaa ggccttaagg gacagcctgc tggaggttgt ttacggcagg
     6961 ttaaaggctt agattaaagg tagatataat gactgccagg ggtgaagaga actaaattct
     7021 tcgccctttt tttctagaag ccaaccctat taatagattg taaggaggct tctaaagtgg
     7081 taaaataagc aggtaagatt gattaggacc attcagaggt gaacatatgt actattttga
     7141 taacggagca accacatttc caaagccaca ggaagtgtat gatgagatat acaaggcaat
     7201 gacagagtat ggtgcaaacc ctggaagatc aggccatgac ctggccctaa gggcttcaag
     7261 ggggatatac gagaccaggg aagggcttgc aaggctattt tccatataca accccatgaa
     7321 tattgtattt acttacaatt gcaccgagag cttgaatatt gccataaagg gcttgcttaa
     7381 gccaggggac catgttatca caacatccat ggagcataat tccgttctta ggcccattaa
     7441 gcaccttgaa acgattggtg tccagaacac gattgtatgg gcacacaagg atgggcagat
     7501 cgacccagca gcggttaggg atgccatcag gaagaacaca agactgattg tgacaaccca
     7561 tgtatcaaac cttaccggta ccatcatgcc tgtgaaggaa ataggcatga tagcaaggga
     7621 acatggaata ccctatcttt tggatgcagc ccagtctgcg ggagtatatc aaatagatgt
     7681 tgaagacttg ggagttgaca tgcttgcttt tccaggacac aagggacttc ttggcccgca
     7741 gggtacggga ggactctata tcaggga
//
LOCUS       DASRQF010000106         2219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_260320, whole genome shotgun sequence.
ACCESSION   DASRQF010000106 DASRQF010000000
VERSION     DASRQF010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260320"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VFD79_02665"
     CDS             <1..467
                     /locus_tag="VFD79_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92980.1"
                     /translation="YNKKTTRAMNEEAIQILRELGIELYATMIIPLDFSKEDFRELTG
                     WLKQMKVTFVNLQPLTPLPGTDIFDEYKDRLLYDTWDHHVFDMAHVVLRPERMSVRMF
                     YFHLLLGYYRVVIRPVNILRLVRRYGLWANLRMLKGSQMVGLQYMKKIVRGY"
     gene            467..1789
                     /locus_tag="VFD79_02670"
     CDS             467..1789
                     /locus_tag="VFD79_02670"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZH92981.1"
                     /translation="MKKLLLIQPSPYDRQGNPIKKKKLYFVGLSLPLLAAMTPSDWEV
                     EIIFEIIEDIPFQTDADLIGISTMGHGVIRSIDIAKEFRRRGKTVIMGGYMASIMPQE
                     AMKYCDSLVEGDAEHVWEDLIQDFERGDLKANYKKPLREGWFSTPKPRFDLIVDKPIG
                     DFLPVQAGRGCPNTCSFCSVACLYKGSYVKKPVQEVKRDLHQVRDLGFGKVLLLDDNI
                     FSDREYLKDLLSILKDMKLEWMSQCDIRIGREDDLLKDLAESGCTTLSFGLESISRES
                     LVAMNKGWARPEDYTELIKNIKRHGIDVSTEMVVGGEGDTLESIRKTKDFIEDNRISV
                     PRFYILTPFPGTSFFRDMETAGRLENRDIYSYDGTAAVHTPANMTAQELTDSYWELYE
                     SLFTIRSIFTRNIMKREFLRDPFRYLFYGMVNLYYRHQIRNRITPNIF"
     gene            1801..>2219
                     /locus_tag="VFD79_02675"
     CDS             1801..>2219
                     /locus_tag="VFD79_02675"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HZH92982.1"
                     /translation="MRILLVRPPRIKQAITLGSFMFSEPLGLEMIYGVLKKDHQVKIL
                     DMMIQDDLPGELKEFSPHIVGITSLCIDVDRVISLAGECKAFDGDIITMVGGTQTLLS
                     PESFFDEGIDHIFRFSTRDNILELMGQIEDGRIEALDG"
BASE COUNT          642 a          471 c          582 g          524 t
ORIGIN      
        1 gttacaacaa gaagaccaca agggccatga atgaggaggc catccagatc ctcagggagc
       61 ttgggatcga gctctatgcc acgatgataa tcccactgga cttctcaaag gaggatttca
      121 gggagctgac aggatggctg aaacagatga aggtcacctt tgtaaatctt cagcctttga
      181 ctcccctgcc ggggactgac atttttgatg aatacaagga cagactcctt tatgacacct
      241 gggaccatca tgtttttgac atggcacatg tggtcctgag gcctgaaagg atgagtgtga
      301 gaatgttcta tttccacctg ctcctaggct attacagggt cgtcattaga cctgttaaca
      361 tccttagact ggtcaggaga tacggacttt gggcaaatct gaggatgctt aagggaagcc
      421 agatggtggg ccttcagtac atgaagaaaa ttgtgagggg atactgatga aaaagcttct
      481 attgatacag ccaagtccat atgacagaca gggtaatccc ataaagaaga agaagcttta
      541 ctttgttggt ctctcccttc cccttctggc agccatgacc ccctcggact gggaggtgga
      601 gatcatcttt gagataatcg aggacatacc cttccagaca gatgctgacc ttattggaat
      661 aagcaccatg ggacatgggg taatccgttc aattgacata gccaaggagt tcagaaggag
      721 gggaaagaca gtaataatgg gtggatatat ggcaagcata atgccccagg aggccatgaa
      781 gtactgcgat tccctggttg aaggggatgc ggagcatgtg tgggaggatt tgatccagga
      841 ctttgaaagg ggagatctca aggctaatta caaaaagccc ctaagagagg gctggttttc
      901 aacacccaag cccagatttg acctgattgt ggacaagccc atcggggact ttcttccggt
      961 ccaggctgga aggggatgtc ccaacacctg cagcttctgt tcagtggcct gcctctacaa
     1021 gggcagctat gtaaaaaagc ctgtccagga ggtaaaaaga gaccttcacc aggtcaggga
     1081 ccttggcttt gggaaggtcc tacttcttga tgacaacatt ttttctgaca gggaatacct
     1141 taaagacctc ttgagtatcc taaaggacat gaagctggag tggatgagtc agtgcgacat
     1201 aaggatcggc agggaggatg acctccttaa agaccttgct gagagtggtt gtaccaccct
     1261 tagctttggg ctggaaagca taagcaggga aagcctggta gccatgaaca agggctgggc
     1321 aaggccagag gactacacag aactgataaa gaacattaaa agacatggaa tagatgtatc
     1381 cactgaaatg gtggttggtg gggaagggga caccttggag tcaataagaa agaccaagga
     1441 tttcattgaa gacaacagga tctccgtccc caggttctat atcctgaccc catttccagg
     1501 aacctctttt ttcagggaca tggagactgc agggagactg gaaaacaggg atatctactc
     1561 ctacgatgga accgcagcgg tccacacccc tgccaacatg actgcacagg agctgaccga
     1621 cagctattgg gagctctatg aatccctgtt taccatcaga tctattttca caaggaatat
     1681 catgaaaagg gaatttctta gggacccctt cagataccta ttctatggaa tggtaaacct
     1741 ctactacaga caccagataa ggaacaggat aacacccaac attttctaag ggaggacaaa
     1801 atgagaatac tattggtaag acccccgaga atcaagcagg ccataaccct tggaagcttt
     1861 atgttttcgg agcctcttgg ccttgaaatg atatatgggg tccttaagaa ggaccaccag
     1921 gtgaagattc tggacatgat gattcaagat gacctcccag gggaactaaa ggaattttcg
     1981 ccacatattg ttggaataac aagcctttgc atagatgttg acagggtcat ttctcttgca
     2041 ggggaatgca aggcctttga cggtgacata ataactatgg tgggaggaac ccagaccctc
     2101 ctgtcccctg agagcttttt tgatgaaggg atagaccata tcttcaggtt ttccacaagg
     2161 gataatattc ttgagctgat gggtcaaatt gaagatgggc ggattgaagc actggatgg
//
LOCUS       DASRQF010000107         2501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_657187, whole genome shotgun sequence.
ACCESSION   DASRQF010000107 DASRQF010000000
VERSION     DASRQF010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657187"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VFD79_02680"
     CDS             complement(<1..473)
                     /locus_tag="VFD79_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HZH92983.1"
                     /translation="MRKTEGLKPDRVFHYFEELTKIPRCSYDEKRVSDYLKEVGEGLG
                     LEVVQDQALNIIIRKPGTKGYEDKPVVIIQGHMDMVCEKADDCDIDFSKDPIPFEVDG
                     DFIVAPKTTLGADNGIAVAMGLAIIESEDIPHPPIELLITTNEESGMTGAKELDV"
     gene            complement(475..1773)
                     /locus_tag="VFD79_02685"
     CDS             complement(475..1773)
                     /locus_tag="VFD79_02685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HZH92984.1"
                     /translation="MNIILELNHIIENSKSLAQKLMEDKREPVRVEDHGDAGRGNRLY
                     LGDNLDSILHLLGDGMRGRIDLVYIDPPFFSGSNYKRRITLGQGEEKLVFYNHAYGDI
                     WEDGIIDYLQMIATRLFLIRDLLSDKGTIYVHVDGRTSHYLRLILDQVFGEERFLNEI
                     IWSYKSGGAGRRSFSKKHDNILVYTKTKDYIFNPIREKSYNRGMKPYRFKNVTEYQDE
                     LGWYTLVNMKDVWSLDMVGRTSSERVGYETQKPETLLRRIIMASSNEDSIVADFFMGS
                     GTTIKVAQELGRKWIGGDTSIHSIVTCKRRLGPDPGLRLHHMEEDTDSNLSFTATASR
                     REKDLEVFIRDMGLDIEAIKKDASDPDLALKMVKDKPSIYIDYLGLYGVDKHWESIGE
                     WYGQEVESRVYLEDFPKDFDRIMIRMIDIFGNIAERELEV"
     gene            complement(1799..2302)
                     /locus_tag="VFD79_02690"
     CDS             complement(1799..2302)
                     /locus_tag="VFD79_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZH92985.1"
                     /translation="MIKVDAVDKMNEEERLKYMLLLLKGQVGSEKDVLANISNVTAII
                     KACVDDLNWAGFYLMKRGELVLGPFQGLPACNRIQVGKGVCGRAIEEGKTQLVPDVHL
                     FPGHIACDSASNSELVVPIIKKGKAYGVLDLDSPSKDRFKELHRVYFERLVDILNQHT
                     DWDRVTE"
     gene            complement(2313..>2501)
                     /locus_tag="VFD79_02695"
     CDS             complement(2313..>2501)
                     /locus_tag="VFD79_02695"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HZH92986.1"
                     /translation="IYYLDEEDIPVIKEKMDQVGEKYREPIVTELEPLRSYFSAEEYH
                     QDYLKKNPGGYCHVDMSL"
BASE COUNT          591 a          691 c          482 g          737 t
ORIGIN      
        1 ttcacatcca gctccttggc tcctgtcatt ccagattcct cgtttgttgt aatcaatagc
       61 tctattggtg ggtgtggaat gtcctctgac tcaataatgg caagtcccat tgcaacagct
      121 attccattgt ctgctcccag ggtggttttt ggagcaacaa taaagtctcc atcaacctca
      181 aaggggattg ggtcctttga aaagtcgata tcacaatcgt ctgccttttc acagaccata
      241 tccatgtgcc cctggatgat cacaacaggc ttatcctcgt atccctttgt tccaggcttt
      301 ctgattatta tgttcagggc ctggtcctgg accacctcaa gtccaagtcc ctctccaacc
      361 tcctttagat aatcgcttac ccttttttca tcgtaggagc atctgggaat tttcgtcagt
      421 tcctcaaaat agtgaaaaac tctgtccggt ttaagtccct cggttttcct catactatac
      481 ctccaattct ctctcggcaa tatttccaaa aatatctatc attctgatca tgatcctgtc
      541 aaagtccttt ggaaaatcct ccaaatagac tctggactca acctcctgac cataccattc
      601 accaattgat tcccaatgtt tatctacccc gtaaaggccg agatagtcga tgtagatgga
      661 tggtttatcc ttcaccattt tcaaggccag gtccgggtct gacgcatctt tttttatggc
      721 ctcaatgtcc aaacccatgt cccggatgaa aacctccaag tccttttccc gtcttgaggc
      781 tgtagccgta aaagacaggt tggaatcagt atcctcctcc atatggtgga gccttaggcc
      841 tgggtcaggt ccaagtctcc ttttacaggt tacaatgcta tggatgctgg tgtccccacc
      901 gatccatttc cttcccaact cctgggccac ctttattgtg gttccgctac ccatgaagaa
      961 gtcggccact atggagtcct catttgagga agccattatt atcctcctaa ggagtgtctc
     1021 tggcttctgg gtctcataac ccaccctttc tgaggaagtc ctaccaacca tgtccagact
     1081 ccatacatcc ttcatattca ctagggtgta ccatcccaat tcgtcctggt actcggtcac
     1141 attcttgaac ctgtagggct tcatcccccg attgtaggat ttctccctga tggggttgaa
     1201 aatataatcc ttggtcttgg tgtagaccag gatgttatca tgctttttgg aaaaactcct
     1261 ccttcctgct ccgcctgatt tgtaggacca tattatctcg ttgaggaacc tttcctcccc
     1321 gaaaacctgg tccaggatca gtctcaggta atgggacgtc cttccgtcca catgaacata
     1381 tattgtaccc ttgtcagaga gcaggtccct tatgagaaaa agtcgggtag ctatcatttg
     1441 gagatagtca atgatcccat cctcccagat atctccgtag gcatggttat agaaaaccag
     1501 cttctcctcc ccctggccaa gggtgatcct ccttttgtag ttggaacctg aaaaaaaagg
     1561 agggtcgata tagacaaggt caatccttcc cctcattcca tcccccagca ggtgaaggat
     1621 ggaatccagg ttatccccaa ggtagagcct gttgccccgg ccagcatctc cgtggtcctc
     1681 tactctgaca ggctcccttt tgtcttccat aagtttctgt gcaagagact tggaattttc
     1741 aatgatatgg ttcagctcaa gtattatgtt cacgtctaca actccgatct gtaaaaacct
     1801 attctgttac cctgtcccag tcagtatgct ggtttaaaat atcaacaagc ctttcaaagt
     1861 ataccctgtg aagttccttg aacctgtcct ttgatggact gtccaaatcc agaaccccat
     1921 aggccttgcc cttctttatg attggaacca caagctccga atttgaagca gaatcgcagg
     1981 caatgtggcc tggaaaaagg tggacatcgg gaacaagctg ggtctttccc tcctctatgg
     2041 cccttccgca aacgcccttg ccaacctgga tcctgttaca tgcaggcagc ccctggaatg
     2101 gtccaaggac caactcaccc cttttcatca gataaaagcc cgcccagtta aggtcatcca
     2161 cacatgcctt gattatagcg gtcacattgg atatgttggc aagcacgtcc ttctctgagc
     2221 ccacctgacc cttcaagaga aggagcatgt acttaagcct ctcctcctca ttcattttat
     2281 caactgcatc tactttgatc atcttatccc tcctacaggc tcatatccac gtgacaatag
     2341 ccacctggat tcttcttcag atagtcctgg tggtattcct ctgcagaaaa atatgaccta
     2401 aggggctcaa gctctgttac aatgggttcc ctgtactttt cccccacctg gtccattttc
     2461 tcctttatca caggaatatc ctcctcatca aggtagtata t
//
LOCUS       DASRQF010000108         4048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_819679, whole genome shotgun sequence.
ACCESSION   DASRQF010000108 DASRQF010000000
VERSION     DASRQF010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819679"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VFD79_02700"
     CDS             <1..319
                     /locus_tag="VFD79_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZH92987.1"
                     /translation="VNLYEDMKDVTLIDFLPTSVEFDMNFHILTFQPGGSHPYIETHV
                     QEHGAYLLSGEGMYNLDNDWISVKKGDFIWMGPFVHQATYSVGYEPLSYVYSKDCNRD
                     PEI"
     gene            complement(356..1996)
                     /locus_tag="VFD79_02705"
     CDS             complement(356..1996)
                     /locus_tag="VFD79_02705"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HZH92988.1"
                     /translation="MGIILKDILKMSCFENSKLVSGIEIIDRTEVEGITIVERPDIAN
                     WIKGGELLLTSFYSIESDVTAQKKLVEDVARNGAAGIIIKVSDVVPVVSNEIIETGKR
                     LNFPVISISKEVKYIDILYPVMGLLFNEQVYKLNYYKECHEKFNRLSLEMKGFESIAK
                     TLDGMVENPVIIFDSEMRILAFSNEQFSELNIVEKKMKKMIYEGYPIYGLDINFEEDE
                     YHLTIEPIEVVNRIQAYLGVLEVTRDMNKMDFIALESAANTLRLEILKETAVNEAKLR
                     YKGDLMDDLISGNYDSVQSIYDRGHILGWDLRKKYLLGLYNISWKNEEKHENNAEWSL
                     ESRNIVKTIIDRIAFHHTTGHISLIKGEELIVFWPVVENESPQTSIKNLKIFGEEVRE
                     TVKKRFGEVFVTMGIGSIAETVKDMGRSYSESKDAANFGKRIFGEDSTSVFDELGIYK
                     MLCSFDDRDALQDFIPESLRKLNSYDKDKNNELIDTFEMYLKCNLNAVKTAEELFVHY
                     KTVLYRLNRIKEITGLDIEDRDKMLEIEVGLKIMKIMR"
     gene            complement(2061..2996)
                     /gene="arcC"
                     /locus_tag="VFD79_02710"
     CDS             complement(2061..2996)
                     /gene="arcC"
                     /locus_tag="VFD79_02710"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004836868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HZH92989.1"
                     /translation="MSKVMIALGGNALGNSPKEQLQLVEKTARPIVDLIEEGHTVVVA
                     HGNGPQVGMINLSFETANLSDSKVPEMPFPECGAMSEGYIGYHLQNAIKNELTTRNIE
                     KSVGTIVTQVIVDEHDEAFDHPSKPIGGFYTEEEAKSLESEKGYSMVEDSGRGYRRVV
                     PSPKPVDIVEIDMIRDMVDSGHVVIAVGGGGIPTLKKDGRLKGVAAVIDKDLASEKLA
                     EMLECDILFILTAVETIALDFGKSTQRELSSMTIAEAEQFIKEGHFVPGSMLPKVMAA
                     VDFVKSHPEREAIITSLESAKAAIHGDTGTRIIPS"
     gene            complement(3012..3692)
                     /locus_tag="VFD79_02715"
     CDS             complement(3012..3692)
                     /locus_tag="VFD79_02715"
                     /inference="COORDINATES: protein motif:HMM:NF022834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HZH92990.1"
                     /translation="MTTNEDVLPPLGIVLSRKGFEEFSIENRKFIISESKVYCSQMDT
                     YDKNPDSRIFNIYMRRLMLDDFENGLGIDNKEVEKILMNTRLTNLTEEGEMIWRMISR
                     RGSVDMDRLIGRGRGLTPAWDDFIVGFSAVLRANSADEEIRQIIQELIFSEGLKLTTD
                     ISQDYLIKTINGCFSQNIKMLINFIQNNIFDEKSYLNVIKYGGTSGVDTFIGMMSAYA
                     SLLGIGTI"
     gene            complement(3808..>4048)
                     /locus_tag="VFD79_02720"
     CDS             complement(3808..>4048)
                     /locus_tag="VFD79_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH92991.1"
                     /translation="SVGHNPNLAIPTWNFKGALVGIDVRKVVETGITPLINTGIAHKK
                     AGVGQIGAGMVRAPYECFTKALEGLVEKYVSEEDN"
BASE COUNT         1059 a          968 c          637 g         1384 t
ORIGIN      
        1 agttaacctt tatgaggaca tgaaggatgt aaccctgata gattttctgc cgaccagtgt
       61 tgagtttgac atgaactttc acattctaac attccagccg ggaggatctc acccatacat
      121 cgagacacat gttcaggagc atggagcata tctcctgtcc ggtgagggca tgtacaatct
      181 ggacaacgat tggatttccg tcaaaaaggg tgattttatc tggatggggc cttttgttca
      241 ccaagcgact tattcagttg gttatgagcc tctttcgtat gtctattcaa aggactgcaa
      301 tcgtgatcct gaaatttaga aaaatcctgg tttccatatg gaaaccagga ttttcttatc
      361 tcatgatttt cattatttta agcccaacct ctatctcgag catcttgtcc ctatcctcaa
      421 tatcaagtcc tgtaatctcc tttatgcgat taagtctata aagcactgtc ttatagtgga
      481 caaaaagctc ctccgccgtc ttcactgcat ttagattaca cttcaggtac atctcaaatg
      541 tatcgatcag ttcattgttt ttatccttat catagctatt caactttctg aggctctcag
      601 gtataaagtc ttgcagggcg tctctatcat caaagctgca aagcatcttg tatatgccca
      661 attcatcgaa tacacttgtt gaatcttctc caaaaatcct cttcccaaaa tttgctgcat
      721 ccttggattc actgtagctc ctacccatat ctttaactgt ttcagcaatg ctacctattc
      781 ccattgtaac aaaaacctct ccaaatctct tctttacagt ctccctaact tcttcgccaa
      841 aaatctttag attctttatt gaggtttgcg gactctcatt ttcaacaact ggccaaaata
      901 ctatcaactc ttctcccttg atcagagaga tatgcccagt ggtatgatgg aaagcgatcc
      961 tgtctataat agttttaaca atgtttcttg actctagaga ccattcagca ttattctcat
     1021 gcttttcttc attcttccag ctaatattat agagaccgag aagatatttc ttcctaaggt
     1081 cccaaccaag gatatgtcct ctgtcataga tgctctggac tgaatcataa tttccactaa
     1141 tcagatcatc cataagatcc cccttgtacc taagcttagc ctcattaaca gctgtttcct
     1201 taaggatttc cagcctaagt gtattggctg cactttcaag ggctataaag tccattttgt
     1261 tcatgtctct ggttacttca agcactccca gataggcctg tattctattt actacctcaa
     1321 tgggttcgat tgtaagatga tattcatcct cttcaaaatt aatatcaaga ccatatattg
     1381 ggtagccttc ataaatcatt tttttcatct ttttctcgac aatattcagt tcagaaaact
     1441 gctcattgct aaaagccagg attctcatct cactgtcaaa gataattact ggattctcca
     1501 ccataccatc tagtgtcttg gcaatggact caaacccctt catctccaat gagagcctat
     1561 tgaatttttc atggcattct ttatagtaat ttagcttgta gacctgctca ttgaaaagca
     1621 aacccataac gggatagaga atgtctatgt atttcacttc tttgctgata gatattaccg
     1681 gaaagttcaa ccttttccct gtttcaataa tttcattgga caccactggt actacatcag
     1741 agacttttat tataattccc gctgcaccat ttcttgcaac atcctccaca agcttttttt
     1801 gagcagttac atcagattca atggagtaaa agcttgttag gagcaactcc cctcctttta
     1861 tccaattggc aatatcgggt ctttcaacaa ttgttatacc ttctacctca gtcctatcaa
     1921 ttatctcgat tcctgaaaca agcttggagt tctcaaagca tgacattttg agtatatcct
     1981 ttaaaatgat tcccatttgt atccctctca atcctatcct aatttaatgc aaacagaccc
     2041 tcctttgggg agggctttgg ttagcttgga atgatccttg ttccagtgtc gccgtgtatt
     2101 gcagccttgg cactctccag ggatgttatg attgcttctc tctcagggtg agattttaca
     2161 aagtccacag ctgccattac ttttggaagc atgctccctg gtacaaaatg accttcttta
     2221 ataaattgct ctgcctctgc tatagtcatt gagcttagct ctctttgtgt tgacttccca
     2281 aagtccagag ctattgtctc tactgcagtt agtatgaaca gtatgtcgca ttccagcatc
     2341 tctgcaagct tctcactggc taggtcctta tcgattacag ctgctacacc ctttagtctt
     2401 ccatctttct ttagtgttgg gattcctcct cctccaactg caatcacaac gtgtccactg
     2461 tccaccatgt ctcttatcat gtctatttca actatatcta caggcttagg tgaaggtacc
     2521 actctcctgt atccccttcc tgaatcttca accattgaat atcccttttc actctccaat
     2581 gatttagctt cctcttcagt gtaaaaaccc ccaattggct tggaaggatg atcaaaagcc
     2641 tcgtcatgtt catccacgat tacctgtgta acaatagtgc ccactgattt ctctatgttt
     2701 cttgtagtaa gttcattctt tattgcgttc tgaagatgat aacctatata tccttcgctc
     2761 attgctccac actctggaaa tggcatttct ggaaccttgc tgtctgacag gtttgctgtt
     2821 tcaaatgaca agttaatcat accaacctga ggtccgtttc catgtgcaac aacaactgta
     2881 tgaccttcct ctataagatc cactataggt cttgctgtct tttccacaag ttgcaattgc
     2941 tcctttggac tatttccaag ggcatttcca cctaaggcta tcataacttt actcacattg
     3001 accactccca ttcatatagt tcctattccc aataaactgg catatgctga catcatacct
     3061 ataaatgtat ccactcctga agtcccacca tattttatca cattaaggta tgacttttca
     3121 tcaaaaatat tgttctgaat gaaattaatt aacatcttaa tattttgact gaaacatccg
     3181 tttattgttt tgatcaaata atcttgagaa atgtctgtcg ttagcttcag gccttcagaa
     3241 aatattaatt cttggattat ttgtcgaatc tcttcatctg cactgttggc tctaaggaca
     3301 gctgaaaatc ctactataaa gtcatcccat gctggtgtta atcccctgcc cctgcctatc
     3361 aacctatcca tatcaactga gcccctcctg ctgatcattc gccaaatcat ttccccttct
     3421 tctgtgagat ttgtaagtct ggtgttcata agtatctttt caacttcttt attatcaata
     3481 cctaatccat tttcaaaatc atccagcatt aatctcctca tataaatatt aaatattcga
     3541 ctatcaggat tcttgtcata ggtgtccatc tgagagcaat agacttttga ttctgatatg
     3601 ataaactttc tattctctat ggagaactcc tcgaagccct tcctgcttaa gacaattccc
     3661 aagggtggca gaacatcctc gtttgtggtc acaaccatca agcctttgtc tgtgataaag
     3721 tttctggtgc ttttaaactc actgtgaatt ttaatgtttt gtggattttc aagtagagga
     3781 ataacctctc catcaattgc aagtggatta attatcttct tctgagacat acttttccac
     3841 cagtccctct agagcttttg taaagcattc atatggtgcc cttaccatac cagcgccaat
     3901 ctgaccaacc cctgcctttt tgtgtgcaat accagtgttg atcaatggtg ttattccagt
     3961 ttccacgacc tttctaacat ctattcctac aagagctcct ttaaaattcc atgtgggtat
     4021 ggcgaggtta ggattgtggc ctactgaa
//
LOCUS       DASRQF010000109         8634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1382395, whole genome shotgun sequence.
ACCESSION   DASRQF010000109 DASRQF010000000
VERSION     DASRQF010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8634
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382395"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /gene="trkA"
                     /locus_tag="VFD79_02725"
     CDS             <1..866
                     /gene="trkA"
                     /locus_tag="VFD79_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HZH92992.1"
                     /translation="ISRDGDLIIPNGATEIQNGDIIHVIGKSDEIEKISRRLGEDYKT
                     KIIRSAMILGGGNVGYYLAQRLEMNKVQVTLVEQDKKRSQELSELLDNVLIIHGDGTD
                     IHLLEEENLRGMDAFIGVTGFDEENLLMALMAKQSGVRKSIAKISRSNYIKIIDRLKI
                     DAAINPVYITASSILKYIRGGKVVSVSLLLGGNAEVTEIIIDKDLPCVGKRLIDLTMP
                     KGMIIGAIVRDNDVIIPKGNTELMANDRIVVFCLSENLEGLKMFFKPKKGGLFGELWN
                     RNKSAGKPADS"
     gene            817..2265
                     /locus_tag="VFD79_02730"
     CDS             817..2265
                     /locus_tag="VFD79_02730"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HZH92993.1"
                     /translation="MNYGIVTRVLGSLLIVEAALMLPSYLIAVSNQGPDKIPFLVTII
                     LTAIIGGLMTRKKSSQPSIGAKEGLAIVALGWVFASIMGALPLYLSGSTANYVDAFFE
                     IVSGFTTTGATILTEIEGLPEGVLFWRSFTHWIGGMGILVFTLALMPALGIGGFQIFK
                     AESPGPVAGKIAPRLRDTAKILYITYVTITLAEVALLSIGGMSIYESFIHTFGTVGTG
                     GFSSRNGSIGDFDSTYIHMVIAFFMVVSGVNFSLYYSFFRGKPTELLKDEEFRLYIKI
                     IAIATIAIAIDLWLVSYHNVGYSLRDAFFQVSSIITTTAYGTADFDLWPTFSKGILLI
                     LMFVGASAGSTGGGMKIIRILVMMKLVKREVGKLYHPRAMMPIKVNNKVVSNETVARI
                     NSFLALYLLLFLFSTLLVSLEQVDLVTAGSAVVAALSNIGPGLELVGPTQNFAFFSMP
                     TKLWLSFLMFLGRLELFTIIALVAPKHWRIDI"
     gene            2340..3623
                     /locus_tag="VFD79_02735"
     CDS             2340..3623
                     /locus_tag="VFD79_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004839651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HZH92994.1"
                     /translation="MLDTLSKLKILSDAAKYDVSCSSSGSSRGNKNGGLGNASQAGIC
                     HSWTDDGRCISLLKILMSNECIYNCAYCVSRTSNDLPRATFTPDEIIDITINFYKRNY
                     IEGLFLSSAVVKSPNHTMERFLEIVRRLREEENFNGYIHLKAIPGADREILDKVAVHV
                     DRLSVNIELPTDEGLKLLAPQKKQKEILLPMTQINQGITRYQEERKVFRSTPKYVPAG
                     QTTQLIVGATRDSDYRILNLSENLYQGYGLKRVYYSAFVPVSNNPLLPQIKAPPLLRE
                     NRLYQADWLLRFYGFQAADLLSEDKPDFDLLLDPKCDWALRNMHLFPLEINRADYELL
                     LKVPGIGVKSARRIISSRRFAKVGYEDLKKMGVALKRAKHFITIDGRHYGISSMDDVK
                     IRSAIIGGSSFGVEQLSIFDLSGGGDVGIRTGLSV"
     gene            3592..4344
                     /locus_tag="VFD79_02740"
     CDS             3592..4344
                     /locus_tag="VFD79_02740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HZH92995.1"
                     /translation="MLVSGLAYLYDGSFEGLLTAIYHAYYDKDDPVAILPFWEEGSNF
                     LYTYKKVETDRDKWNRVYGAIQDKISHYSLKRIYNVYLSEMEDSGIIILRYLREAFRI
                     GSGIDDYQTNPAVRDLEGIYHKVRLEVHRFLGLVRYKRLENGIFYAQIEPDHNVAALL
                     APHFVDRIPSERWIIHDVKRGVAVFYDTREWTIRSIEEPSDLVLYEDEEDYQDMWREY
                     YRSANIESRRNKRQQRAYMPVRYWKNLVEKGD"
     gene            4348..5511
                     /gene="alr"
                     /locus_tag="VFD79_02745"
     CDS             4348..5511
                     /gene="alr"
                     /locus_tag="VFD79_02745"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HZH92996.1"
                     /translation="MDLTRPAWLEIDLDNLHFNYKEIRKLVRDTADIMAVVKADAYGH
                     GAAAIVRELMALGVKRFGVAHLSEAIHIKNHSPHAQVLVMGYTPDYLAEVAINRGVRL
                     TVYLEDQADHFAKVAKELDKDAVVHLKIETGMNRLGFLPDEKTLQAVERIKEMDNIII
                     EGMFTHFPAADDDHGYTERSMAKFREFCRMLYEKDIDIPVKHVSNSAAIMNFPQYGLD
                     MVRAGIILYGIYPYPQAKRETLPLKPCISLKAQISHVKTIEKGDKVSYGLTFEASTRT
                     QVATVPVGYADGWSRDLSNKGYALVNGRRVSIIGRICMDQLMLDVTGMDVKRGDEAVL
                     MGSQGEEEITIEEIASQIGQIPASVLCMLTKRLPRVYVKERRVPEVVDYLLEI"
     gene            5522..6199
                     /locus_tag="VFD79_02750"
     CDS             5522..6199
                     /locus_tag="VFD79_02750"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009431685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZH92997.1"
                     /translation="MKRLFENSWQEVLKDEMEKEYYLELRRFLISEYKTNTVYPDKHS
                     IFNALHYTDYNDVKAVIIGQDPYHGPGQAHGLCFSVKPGVTPPPSLVNIYKELRNDLG
                     YEIPNHGYLEKWARQGVLMINTVLTVRAGQANSHRNKGWEIFTDRIIQLLNEREEPMV
                     FILWGSQAIAKEGMIDNSRHHIIKSPHPSPLAAYRGFFGSKPFSRTNEFLQSNCMDPI
                     DWRIEKV"
     gene            complement(6243..6950)
                     /locus_tag="VFD79_02755"
     CDS             complement(6243..6950)
                     /locus_tag="VFD79_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZH92998.1"
                     /translation="MNITIDSLTKKYNNKAVLDIPQMEFQEGQLWGILGPNGSGKTTM
                     VRIIAGILDSCSGNVLYQGEEYSDSIKMAMTLVFQRPYLIRSTVYENIAYPLRIRGVS
                     EQEISTRVDELIESFNIQSIRDQKAWTLSGGEAQKVALARALVFKPRILILDEPTSNI
                     DPESIMQLEEKILDYYRKEKPTILMVTHNIQQAKRLCNRIAFMNEGKIIDSGSPSDIF
                     GSGAMPKIEEFIKKGLI"
     gene            complement(6952..7641)
                     /locus_tag="VFD79_02760"
     CDS             complement(6952..7641)
                     /locus_tag="VFD79_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014969031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZH92999.1"
                     /translation="MDNFVSGFQDAIQLIISLDPEFFEIVRLSMVVSLTATFLAAIIA
                     IPFGTWLGIKEFPGKRILVRIIYTLMSMPPVVIGLVLFLLFLRNGPLGEFRLNFTATA
                     MIIAQFVLVTPIITGVVFNGTKDRGPDIQALARTMGASPTQTVWLLIKELRINILSAV
                     VTGYGRAMSEVGAVMVVGGNIKGHTRIMTTTIAMLRNMGEYSLAIATGIVLLLISFII
                     NSLLYHWQQER"
     gene            complement(7726..>8634)
                     /locus_tag="VFD79_02765"
     CDS             complement(7726..>8634)
                     /locus_tag="VFD79_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014969032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZH93000.1"
                     /translation="NLLILLLVLALPLVTACSTTEPAPAEEPAPVEEPAEEPAEKPQP
                     ADDYQELTNTELILATTTSTENSGLLDFILPIFNEMFGIETKVVAVGTGAALQMGVDG
                     EADVLLAHAKANEEELVANGDTLERFDVMYNDFIIVGPPSDPAGLAENAPADVNAGYV
                     LIAENEAPFVSRGDNSGTHIMELSLWESSGVGTPAGDWYIEAGQGMGDVILMASEMEA
                     YTMTDRATYLAMKDDVDLEIVLEGDETLFNQYGVMAVNPDKGDHINYPAAERFVDWIL
                     APETQELISEFGVEEYGEPLFFPNAK"
BASE COUNT         2413 a         1856 c         2100 g         2265 t
ORIGIN      
        1 ccatatcacg ggatggggac cttataattc caaacggtgc cactgaaatc caaaatggag
       61 acataattca tgtcattgga aaatcagatg aaattgagaa gataagcaga aggctgggtg
      121 aagactacaa gaccaagatc ataaggtctg caatgattct tgggggaggc aatgtgggct
      181 actaccttgc ccaaagattg gaaatgaaca aggtccaggt gactcttgtt gaacaggaca
      241 agaaaaggtc tcaggagctt tcagagctgc tggacaacgt acttatcata catggtgacg
      301 gtacggacat acaccttttg gaggaggaaa acctcagagg tatggatgcc ttcattggag
      361 taacgggatt tgacgaggaa aacctcctta tggcccttat ggccaagcag tctggagtta
      421 gaaaatcaat tgcaaaaatc agccgttcca actacatcaa gattattgac aggttaaaga
      481 tcgatgcggc cataaatcct gtatatataa ccgcaagcag cattttaaag tatataagag
      541 gtggcaaggt ggtctcggtt tccctgctcc tgggaggaaa cgctgaggtt acagagataa
      601 tcattgacaa ggatcttcct tgcgtgggca aaaggcttat agacctaaca atgccaaagg
      661 gaatgatcat cggagccata gttagagata atgatgtaat cattccaaag ggtaacaccg
      721 aactaatggc caatgacaga attgttgtat tctgcctgtc ggaaaatcta gaaggcttga
      781 agatgttctt taaaccgaaa aagggaggtc tgttcggtga attatggaat cgtaacaaga
      841 gtgctgggaa gcctgctgat agttgaagcg gctctgatgc ttccgtcata tctgattgca
      901 gtatcaaacc aggggcctga caagatcccc tttctagtga ctattattct tactgcaata
      961 attggcgggt taatgactag aaagaaaagt agccagccct ccattggtgc caaggaaggc
     1021 ttggccatag ttgctctggg ttgggtattt gcttcgataa tgggggctct tcccttgtat
     1081 ctatcgggct cgacagccaa ttacgtggat gcattttttg agatagtttc ggggtttaca
     1141 accactgggg ccacaatcct cactgaaatt gagggcttgc ctgagggcgt attgttctgg
     1201 aggtctttta cccactggat agggggaatg ggaatactag tattcacact ggcccttatg
     1261 cccgcgctgg gaattggagg attccagata ttcaaggctg agtccccagg acctgttgcc
     1321 gggaaaatcg caccaagact tagggataca gcaaagatac tatatataac ctatgtgacc
     1381 ataacattgg cagaggtggc cctcctttca atcggtggta tgagtattta tgaatccttt
     1441 atacacacct ttggaactgt tgggactggt ggtttttcca gtaggaacgg aagtatagga
     1501 gactttgaca gcacctacat tcacatggtc atagcatttt tcatggtggt ttcaggtgtt
     1561 aacttttcac tctactattc gtttttcaga gggaaaccta ctgagctttt aaaagatgag
     1621 gaattcaggc tttacattaa gatcattgca attgcaacaa tagcaatagc cattgacctg
     1681 tggcttgtaa gctaccacaa tgtaggctac tccttaaggg atgccttctt ccaggtttca
     1741 tcaatcataa ccaccactgc ctatggaacg gctgattttg acctttggcc aaccttcagc
     1801 aagggtatcc tgcttatact aatgtttgtg ggggccagtg caggctctac gggtggaggt
     1861 atgaagatta taaggatcct tgtaatgatg aagcttgtaa agcgggaggt gggtaagctt
     1921 taccacccaa gggccatgat gccaattaag gtgaacaata aggttgtatc caatgaaacc
     1981 gtcgcaagga taaacagttt tctggccttg tacttgctgc ttttcctttt ttccacacta
     2041 ctggtttctc tggagcaggt ggaccttgtg accgcaggaa gtgcagtggt tgcagcactg
     2101 agtaacatag ggccaggttt ggaactggtc ggaccaactc agaattttgc atttttctcc
     2161 atgccgacaa aactctggct ttcatttctt atgtttttgg gaaggcttga gctgtttaca
     2221 ataattgcac tggttgcacc caagcattgg aggatcgaca tttaatcgaa caaatgtttg
     2281 cttgctttat actgatatga tataattgcc tcaagaaaat ttttcttgag gtgtttttta
     2341 tgcttgatac tctctccaaa ctaaaaatcc tatcagatgc ggcaaaatac gacgtttcct
     2401 gttcctccag cggaagtagc agagggaaca aaaatggcgg acttggcaat gccagccagg
     2461 ctgggatttg tcattcctgg acagatgatg gcagatgcat atccctgctt aagattctaa
     2521 tgtccaatga atgcatctac aattgcgcat attgcgtcag caggacctcc aatgaccttc
     2581 ccagggccac ctttacacct gatgagataa ttgacatcac cataaatttc tacaagagga
     2641 actacataga gggcttgttc ctaagctctg ctgtggtgaa atctccaaac cacaccatgg
     2701 agaggtttct ggaaatcgtg agacggctac gtgaggagga aaacttcaac ggttacattc
     2761 acctcaaggc aatccctgga gccgacaggg aaatcctgga caaggtggcg gtccatgtgg
     2821 acaggttaag cgtcaacatc gagctgccta ctgatgaggg cttaaagctc ttggctcccc
     2881 aaaagaagca aaaggagatc ctcctcccga tgacccagat caatcagggg atcacaagat
     2941 accaggagga gagaaaggtc tttagaagca ctcccaaata cgtgcctgca ggacagacca
     3001 cccagcttat tgtaggtgca acaagggatt cggactacag gattctcaac ctttcagaga
     3061 acctatacca gggatacggc ctaaaaaggg tctactactc agcctttgtt cccgtatcca
     3121 acaatccgct tcttccacag atcaaggcac ctccactttt aagggagaac agactctatc
     3181 aggctgactg gctccttaga ttctacggat ttcaggcagc agatcttcta agtgaggaca
     3241 agccggactt tgaccttcta cttgatccta aatgtgactg ggcgcttaga aacatgcacc
     3301 tctttccctt ggaaataaac agggccgatt atgagcttct attgaaggtg ccgggaatcg
     3361 gagtcaaatc tgcaaggaga ataatcagca gcagaaggtt tgcaaaggtt ggctatgaag
     3421 acctaaagaa gatgggagtt gccttaaaga gggcaaagca tttcataacc atagatggca
     3481 ggcactacgg aatctcctcc atggatgatg tgaaaatcag gagtgccatt atcgggggaa
     3541 gctcttttgg tgttgaacaa ctgtcaatat tcgatctttc gggaggtgga gatgttggta
     3601 tcaggactgg cctatctgta tgatgggtcc ttcgaaggac tgcttacagc aatatatcat
     3661 gcttattacg acaaggatga ccctgtggcc attcttccct tctgggagga agggtccaat
     3721 tttttataca cctacaagaa ggtggaaact gacagggaca agtggaacag ggtctacgga
     3781 gctatccagg ataagatttc ccactattcc ctgaagagaa tatacaatgt ttatctttcg
     3841 gaaatggagg atagtggtat aattatcttg agatacctga gggaggcttt tagaatcggt
     3901 tcagggattg atgattacca gaccaatccc gccgtaaggg acctggaagg aatatatcat
     3961 aaggtgcggc tggaggtaca caggtttttg ggcctggtta ggtacaaaag attggaaaac
     4021 ggaatcttct atgctcagat tgaacctgac cacaatgtgg cagcactttt ggcgccccat
     4081 tttgtagaca ggataccatc tgaaagatgg ataatccacg atgtgaaaag aggagtggct
     4141 gtattctatg acaccaggga atggaccatc agaagtatag aggaaccttc ggacctggtg
     4201 ctctatgagg atgaggagga ctatcaggat atgtggaggg agtactacag gagtgcaaac
     4261 atagagagta gaaggaacaa acgtcaacaa agggcatata tgcctgtacg atattggaag
     4321 aacctggttg agaaggggga ttagattatg gatttgacaa gacctgcatg gctggagatt
     4381 gacctggata atctccactt caactacaag gaaatcagaa agcttgtaag ggacacggct
     4441 gatataatgg ctgttgtcaa ggctgatgcc tatggacatg gagcggcagc aatagtaagg
     4501 gagcttatgg cccttggagt taaaaggttt ggggttgccc acctatccga agcaattcac
     4561 ataaagaacc actctccaca tgcccaggtg ttggtgatgg gatatacccc tgattacctt
     4621 gcggaggttg caataaacag gggagtgagg ttgactgtct accttgagga ccaggcagac
     4681 cacttcgcca aggtagcaaa agaactggac aaggatgcgg ttgttcacct aaagattgaa
     4741 actggtatga atcgtcttgg gtttttgcct gatgaaaaaa cactccaggc cgttgaaaga
     4801 ataaaggaaa tggacaatat aataatcgaa ggtatgttta cacacttccc agcggcagat
     4861 gacgaccacg gctacactga aaggtccatg gcaaaattca gggagttctg caggatgctt
     4921 tatgagaagg atatagacat tccggttaag cacgtcagta acagtgcggc aatcatgaat
     4981 tttccccagt atggcttgga catggtcagg gccgggatca ttctttatgg aatctatccc
     5041 tatccccagg ctaagaggga aacgcttccc cttaagccct gcattagcct caaggcccag
     5101 atttcccatg ttaagaccat cgaaaaggga gacaaggtaa gctatggcct gacatttgaa
     5161 gcaagcacca ggacacaggt tgcaactgtc ccagttggct atgccgatgg ttggagcagg
     5221 gatttgagca acaagggata cgccctggtg aatggaaggc gggtctccat aatcggtagg
     5281 atctgcatgg accagctgat gcttgatgtg acaggaatgg atgtaaagcg tggtgatgaa
     5341 gcggtactta tgggaagtca gggagaagaa gagataacaa tagaggaaat tgcaagccag
     5401 attggccaga tacctgcctc ggtcctatgc atgctcacaa agaggctgcc aagggtgtat
     5461 gtcaaggaaa gaagggtacc tgaagtggtg gattatctcc tggaaatcta aggggtggac
     5521 tatgaaaaga ctatttgaaa acagttggca ggaagtacta aaggatgaga tggaaaagga
     5581 atactatttg gagcttaggc ggtttctgat cagtgagtac aaaaccaata cagtttatcc
     5641 ggacaaacac agcatattca acgcccttca ctacacagac tacaatgatg tgaaggctgt
     5701 tataatagga caggacccct atcacggacc cggccaggcc catggcctct gcttcagcgt
     5761 caagcctggt gtgactcctc ctccttcact tgtaaacata tacaaggaat tgagaaacga
     5821 tttgggatac gagatcccca accatggcta tcttgaaaag tgggccagac aaggagttct
     5881 tatgataaac acagttctga ccgttagggc agggcaggca aattcccaca ggaacaaagg
     5941 ctgggagatc ttcaccgaca ggataatcca gctccttaat gaaagggaag agcccatggt
     6001 ctttattctg tggggaagcc aggcaatagc caaggagggg atgattgaca atagcaggca
     6061 ccacataatc aaatctcccc atccaagtcc attggcagcc tacagggggt ttttcggatc
     6121 caaaccattt tcaaggacaa atgaattcct ccaatcaaat tgtatggacc caattgattg
     6181 gagaattgaa aaagtgtaat aagattaaaa gacccaaccg tatataaatg gtggggtctt
     6241 ttttagatca gtcctttttt gatgaattcc tctattttcg gcattgcacc agagccaaaa
     6301 atatcagagg gactgccact gtcgatgatt tttccttcat tcatgaatgc aatccgattg
     6361 caaagcctct tggcctgctg gatgttgtgg gtaaccataa gtatggtagg cttctctttt
     6421 ctgtagtagt caagaatttt ctcctccagc tgcattattg attcagggtc tatgtttgat
     6481 gtgggttcat ccagaataag aatcctgggc ttgaatacaa gggcccttgc aagggccacc
     6541 ttctgggctt caccccctga aagggtccag gccttctggt ctcggataga ttggatatta
     6601 aatgattcta tcagctcatc caccctggtg gatatttcct gctccgaaac tcccctgatc
     6661 cgcaaagggt aggcaatgtt ttcatagaca gttgacctta taaggtaagg cctctgaaac
     6721 acaagggtca tggccatttt tatggaatcg ctgtactctt caccctgata cagtacattg
     6781 cctgagcagg agtccaaaat tccagctatt atcctgacca ttgttgtctt tccagaccca
     6841 tttggaccaa ggattcccca aagttggcct tcctgaaact ccatttgtgg gatgtccaaa
     6901 actgccttgt tattgtattt ttttgtaagt gagtcgattg taatattcat atcaacgctc
     6961 ctgctgccaa tgatatagca gtgaatttat tataaaggat atcagtaaaa gaactatccc
     7021 cgtagcaatg gcaagggaat actctcccat gtttcttagc atggcaattg tggtggtcat
     7081 tatacgggta tggcccttga tatttccacc gacaaccata acagccccta cctcggacat
     7141 ggccctgccg tagcctgtta caacggctga cagtatattt atcctcaact ccttgataag
     7201 gagccagacg gtctgtgttg gacttgctcc catggttctt gcaagggctt gaatatcagg
     7261 gcccctgtcc ttagtcccgt tgaacaccac gcctgttata attggtgtta caaggacaaa
     7321 ctgagctatt atcatggctg ttgcagtaaa gttcagcctg aactctccca gaggaccatt
     7381 ccttaggaat aggaggaaaa gtaccaagcc tattacaaca gggggcatgc tcatcagggt
     7441 atagattatc cttaccagaa tcctctttcc tgggaattcc tttattccca gccaggtacc
     7501 gaagggaatg gcaatgatgg cagcaagaaa tgtggctgta agggatacaa ccatggacag
     7561 tcttactatt tcaaagaatt ctgggtcaag acttatgatt agctggatgg catcctggaa
     7621 gccacttaca aaattatcca tagaaaactc cttaatactg ccccttccgg ggcagtatga
     7681 tttaaatcat ttactttcgt gacctactta aaatctgaag ttatactatt tggcgtttgg
     7741 gaagaaaagt ggttccccgt attcctcaac tccaaactcc gagatcagtt cctgggtttc
     7801 cggtgcaagt atccagtcta cgaacctttc ggctgctgga tagtttatgt ggtctccctt
     7861 gtcggggttg accgccataa ctccgtactg gttaaatagg gtctcgtcac cctcaaggac
     7921 tatttcaagg tctacatcat ccttcattgc caaataggtt gccctgtcag tcatagtgta
     7981 tgcttccatt tcacttgcca taaggataac atcacccatc ccctgaccag cttctatata
     8041 ccaatcgcct gcaggtgtac caactcctga ggattcccac agtgatagtt ccattatgtg
     8101 ggttcctgaa ttgtctcccc ttgatacgaa aggagcttca ttttcagcaa tcaatacata
     8161 accagcgttt acatcggctg gtgcattttc cgccaggcct gctgggtctg atggagggcc
     8221 aactattata aagtcattat acataacatc aaatctctca agggtgtctc cattggctac
     8281 aagttcttcc tcatttgcct ttgcatgggc aaggagcacg tctgcttccc catccacacc
     8341 catctggagg gctgcccctg tacctactgc aacgaccttg gtttcaatac caaacatctc
     8401 attgaagatt ggcaggatga aatcaagcaa gccgctattt tctgtactgg ttgtggtggc
     8461 aagaatcagc tctgtgtttg tgagttcctg gtaatcgtca gctggctgag gcttttctgc
     8521 cggttcttct gccggctctt caactggcgc aggttcttct gccggagcag gttctgttgt
     8581 gctgcaggct gtcaccaaag gcaatgcaag taccagcagc aaaataagta ggtt
//
LOCUS       DASRQF010000110         6197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_388661, whole genome shotgun sequence.
ACCESSION   DASRQF010000110 DASRQF010000000
VERSION     DASRQF010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6197
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388661"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VFD79_02770"
     CDS             <1..480
                     /locus_tag="VFD79_02770"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HZH93001.1"
                     /translation="SLIKANRQNLKDRAKGLVEEAKEKDREIGRLKDKLSANIADEIL
                     AQAQDIKGVKLITHISSDLDMNGLRNLGDELKSRLGSGVVVLGSTGGDKVSLVAMATK
                     DVTGLGVHSGNIVKQVAQATGGSGGGRPEMAQAGGKDKSKLAEAMESVKEILSSQIK"
     gene            553..804
                     /locus_tag="VFD79_02775"
     CDS             553..804
                     /locus_tag="VFD79_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HZH93002.1"
                     /translation="MNETMKFEAAKETPAEPREILFSVFDSLEEKGYNPINQIIGYIL
                     SGDPTYITSHNNARSLIRKIERDELLEEILRAYLKCTKD"
     gene            883..1830
                     /locus_tag="VFD79_02780"
     CDS             883..1830
                     /locus_tag="VFD79_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZH93003.1"
                     /translation="MEMTKLGRSGIEVSRLCFGSLTMTPFQANLSEEEGAYLIQYAFE
                     KGINFLDTAEIYENYGYIKKALKGIKREDYRISTKTYAYTRELAQESLETAMREIGTD
                     YIDIFLLHEQESTHTVRGHYPAIDYLLKAKEAGKIGALGISTHKIAGARALRDYPELD
                     ILHPIVNMKGIGIHDGDIEDMLTELEFLSSNGRGIYGMKPLGGGHLIRDARESLNFVL
                     QKPYIHSVAIGMQSTDEIDANIQLAQNGYIEKEVENRLKKSNRRLIVQDFCQACGNCV
                     DRCQQGGITLVDGMARPNDKCILCGYCATVCPEFCIKVI"
     gene            1841..2260
                     /gene="ruvX"
                     /locus_tag="VFD79_02785"
     CDS             1841..2260
                     /gene="ruvX"
                     /locus_tag="VFD79_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HZH93004.1"
                     /translation="MERILGLDVGTKRIGVAISDPLGFTAQGVMTIERIGMKKDMAAI
                     EDLLADYQVKKVVVGMPKNMNGTIGPSGEMAKKVGEKIRNKFNLEVIYVDERLSTVSA
                     ERVLIQGDVRREDRKKYVDKIAATYILQTYLDMIYKG"
     gene            2273..2521
                     /locus_tag="VFD79_02790"
     CDS             2273..2521
                     /locus_tag="VFD79_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019034867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HZH93005.1"
                     /translation="MTERHTFYDELGNEIEFVVKAKFSLDDTDYVALLPANEIDAMTY
                     ILRIEMDDEGDEVLVGIDDDELEEAKDVYEELVKENLQ"
     gene            2562..2981
                     /locus_tag="VFD79_02795"
     CDS             2562..2981
                     /locus_tag="VFD79_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009431881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HZH93006.1"
                     /translation="MEKLQKEGYKYTNQREEVLRILLNHKDSHMSCDEVHGIVSNERK
                     DVGIATVYRTLQLFEKLGIVYRINFDDGVSRYELNFGTENHHHHHLICTNCGSVTEVK
                     LDLLESLEDEIEKEEGFTIQDHNVKFYGICKDCKNKK"
     gene            3014..4675
                     /locus_tag="VFD79_02800"
     CDS             3014..4675
                     /locus_tag="VFD79_02800"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HZH93007.1"
                     /translation="MAKQNKLKIIPLGGLGEIGKNITVHEYKDSIVIVDCGMTFPEDE
                     MLGVDVVIPDISYLIKNKEKIKGLVLTHGHEDHIGAIPYVLKKLDMPIYGTRMTLGLL
                     RNKLKEHNLKNVDMNEVSPGDSIKLGHFNVEFIRTTHSIPGSAAIALTSPIGTVIQTG
                     DFKVDFTPINGETTDLNRFAELGNKGVLALLSDSTNATRPGYTMSEQTVGETFRDLFY
                     KAEGRIIVATFASNVFRVQQVIDAAEMNGRKVVLSGRSMINVTTVASDLGYLRHNEDT
                     IIDINEMHKYKKDELVLLMTGSQGEPMSALTRVAAGEHRKIELYKDDLVILSATAIPG
                     NEKAVSKTINRLVEKDINVIYESLADIHVSGHACQEELKLIHNLVKPKFFIPVHGEPK
                     HLKIHAELSKSMGTPEENIFVMDTGQTLEFTKNTASIGPSVPSGHILVDGLGVGDVGN
                     IVLRDRKHLSEDGLLVVVITMNKKEGKVAAGPDIVSRGFVYVRESEDLMEEAKKVVAK
                     TLKTCESKKISDWSTIKSNVRDDLRSFLYSTIKRNPMILPIIMEV"
     gene            4692..4982
                     /locus_tag="VFD79_02805"
     CDS             4692..4982
                     /locus_tag="VFD79_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93008.1"
                     /translation="MGTRLLMYLAVILLGAIIGAKAKLGDNLKKRLGSIQTACLLLLL
                     LTMGVMIGMDKDVINSFFSIGYSAIVISIFTVGFSILGVKLVSGYVTGGGKR"
     gene            4979..5572
                     /locus_tag="VFD79_02810"
     CDS             4979..5572
                     /locus_tag="VFD79_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HZH93009.1"
                     /translation="MTGKIVGAVAAGVLLGLYVLPVTILDHTDMIIDIGLMLLLFFVG
                     IEIGLNRSVFTRIRSMGLKILLIPLMIITGSVVGGIVGGSLLGMPFNESGAVGAGLGW
                     YTLSSILMANYSNELSTLAFLANVVRELMAFMIIPLVAKRVGFLSAIGPGGATSMDTA
                     LPLVSKSTDPETAIIAFISGAICTFSVPILVPLILNL"
     gene            5593..6084
                     /locus_tag="VFD79_02815"
     CDS             5593..6084
                     /locus_tag="VFD79_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HZH93010.1"
                     /translation="MKIRYITKASLIAAIYLVLILIQALPFPVANLTFGPVQLRIAEG
                     LTLLPLMEPAAVPGLFIGVLLANFLLAPYSGFGLVDIIGGSLVTLLAAYLTSKAKNKY
                     VGMVPPILLNGIIVSIWVSYFTRIPYIYTALGITAGQFISVVVFGSLILYVYEKASKY
                     REW"
BASE COUNT         1967 a         1225 c         1603 g         1402 t
ORIGIN      
        1 tcattgatca aggcaaacag gcaaaacctt aaggacaggg ccaagggcct tgttgaggaa
       61 gccaaggaaa aggacaggga gattggcagg ctcaaggaca agctgtcagc aaacatagct
      121 gatgagatcc tcgcccaggc ccaggatata aagggcgtca agcttatcac ccacatctcg
      181 tcagacctgg acatgaacgg tctgaggaat cttggagacg agcttaagtc aagactgggg
      241 tcaggagttg ttgtacttgg atccactgga ggggacaagg tatccctggt tgcaatggca
      301 acaaaggatg ttaccggact tggggtccat tcaggaaaca tcgtcaagca ggttgcccag
      361 gcaacaggcg gaagcggtgg aggaagacca gagatggcac aggctggagg caaggacaag
      421 tccaagctgg ctgaggcaat ggaatcggtt aaggaaattc tttcaagtca gataaaatag
      481 ccataaaaaa agcttccaat gatggagact tatgttataa tagatgagag gggtgatagt
      541 gtggatgaga aaatgaatga aaccatgaag tttgaggcag caaaggaaac accggcggaa
      601 cctcgtgaaa ttctattctc tgtgtttgat tccctggagg aaaagggata caatcccatc
      661 aatcagataa tagggtatat actatcaggg gaccctacct atattacaag ccataacaat
      721 gcaaggtcgt taattagaaa aatcgagcgg gacgagttgt tggaggaaat acttcgagca
      781 tacttaaaat gcactaaaga ttagatataa tggatggatg gctgacacag gctatccatc
      841 cttatttatg atatgatata tataaaggga gctgtaggga aaatggaaat gacaaaattg
      901 ggcaggtcag gaattgaagt gtcaagactt tgcttcggat cccttacaat gacacccttc
      961 caggcaaacc tgtcagagga ggagggagcc taccttatcc agtatgcctt cgaaaagggt
     1021 ataaacttcc tggatacggc agaaatatac gaaaactacg gctacatcaa aaaagcccta
     1081 aagggaatca aaagagagga ctacaggatt tccaccaaga cctatgccta tacaagggaa
     1141 ttggcccagg agagcctgga aacggccatg agggaaatag gcacggacta tattgacata
     1201 tttcttttac acgagcagga gagcacccac acagtaaggg ggcattaccc tgcaatagac
     1261 tacctcttaa aggccaagga agcgggcaag atcggggcat tgggaatttc cacccacaag
     1321 attgctggag caagggcctt aagggactac ccggaacttg acatcctaca ccccatagtt
     1381 aacatgaagg ggatagggat acacgatggg gacattgaag acatgctcac tgagcttgaa
     1441 ttcctaagct caaatggaag agggatatat ggaatgaagc cccttggagg gggacacctg
     1501 atccgggatg caagggaatc cctaaacttt gtccttcaga agccatatat acattcagtt
     1561 gcaataggga tgcaatcaac cgatgaaata gatgcaaata ttcagttggc ccaaaatggg
     1621 tatatagaaa aggaagttga gaacaggcta aagaagagca acaggagatt gatcgtacag
     1681 gacttttgcc aggcctgcgg aaattgcgtg gacaggtgcc aacagggagg aattaccctg
     1741 gtggacggga tggcaaggcc caatgacaaa tgcatacttt gcggatattg tgcaacagta
     1801 tgtccggaat tttgcataaa ggtgatataa tggagataat atggaacgaa tactaggatt
     1861 ggatgtagga acaaagagaa tcggagttgc aataagcgac cccttgggat ttactgccca
     1921 gggagtaatg accatcgaaa gaattgggat gaaaaaggat atggcagcca ttgaggacct
     1981 tcttgcagac taccaggtga agaaggtcgt agtgggtatg ccgaagaaca tgaatgggac
     2041 catcggccca tcaggggaga tggcaaaaaa ggttggagag aaaatcagga ataagttcaa
     2101 tttggaagtt atctatgtgg atgagaggct aagcacggtc tctgcggaga gggtcctgat
     2161 acagggagat gtccgcaggg aggacaggaa gaagtacgtg gacaaaatag cggctacata
     2221 cattctgcaa acttatcttg atatgatata taagggctaa aggaggagaa caatgactga
     2281 gagacacacg ttctacgatg aacttggaaa tgagattgaa tttgttgtaa aggcgaaatt
     2341 cagcttggat gacactgact atgtcgcgct tttaccggca aatgaaatag atgcaatgac
     2401 atacatcctg aggattgaaa tggatgatga aggagacgaa gtactggtag gaatagatga
     2461 tgacgaactt gaggaagcaa aggacgtcta cgaggaactg gttaaggaaa acctacaata
     2521 acaggcgggt gatgactgtg gaaaccacga taaagcaatt tatggagaag cttcaaaagg
     2581 aaggatacaa gtacacaaac cagagggaag aggtcctaag gatcctactc aaccacaagg
     2641 actcccacat gagctgtgac gaagttcacg gcattgtatc caatgaaagg aaagacgtgg
     2701 gaattgccac agtttacaga accctgcagc tttttgaaaa gctgggaata gtatacagga
     2761 ttaattttga cgatggtgta agtcgttatg aactgaattt cggaacggag aaccaccacc
     2821 atcatcattt aatatgcaca aattgcggaa gtgtgaccga agtgaaactg gacctgttgg
     2881 aatcccttga ggatgaaatc gaaaaggaag aagggtttac aatccaggac cacaacgtca
     2941 aattctacgg aatatgcaag gactgcaaga ataagaaata gaaagccaaa tagaaaaaac
     3001 gaggagtgat aaagtggcaa agcaaaacaa attaaaaata atccctttag gtggattagg
     3061 tgaaatcggg aagaacatca ccgtacacga gtacaaggat tccatagtca tagttgactg
     3121 tggaatgacc ttcccagagg acgaaatgct gggtgtggat gttgtaatac ctgacatatc
     3181 ctaccttata aaaaataagg aaaaaataaa gggactggta ttgacccatg gtcatgagga
     3241 ccacataggg gctattccct atgtcctgaa gaaactggac atgccaatct acggtaccag
     3301 gatgaccctg ggcctcttga ggaacaagct taaggaacac aatctaaaga atgttgacat
     3361 gaacgaggtt tccccaggag actcaatcaa gcttggacat ttcaatgtgg aattcatcag
     3421 gactacccac agtattccag gtagtgctgc aattgcattg acaagcccaa ttggaactgt
     3481 aatccagacc ggggatttca aggttgactt cacaccaata aacggtgaga caacagacct
     3541 taacaggttc gccgagcttg gaaacaaggg agttctggcc ctactgtcag actccacaaa
     3601 cgcaaccaga cctggctaca caatgagtga gcagacagtt ggagagacct tcagggacct
     3661 tttctacaag gcagagggaa ggatcatcgt tgcaaccttc gcatcaaacg tcttcagggt
     3721 ccagcaggta attgacgctg cggaaatgaa cggccgtaag gttgtccttt ccggtaggtc
     3781 aatgataaat gtaactacgg ttgcatcaga ccttggatac cttagacaca acgaggatac
     3841 aataatcgac ataaatgaaa tgcacaagta caagaaggat gagctggtcc tccttatgac
     3901 aggttcacag ggagagccaa tgagcgccct tacaagggtg gcggcaggtg aacacaggaa
     3961 gatcgagctt tacaaggacg acctggtaat cctttcagca actgcaattc caggaaatga
     4021 aaaggctgta tccaagacaa tcaacagact ggtagagaag gacataaatg tgatctacga
     4081 gtcacttgca gacattcacg tttcaggcca tgcctgccag gaggagctga agctgatcca
     4141 caacctggtc aagccaaagt tcttcatacc ggtacacggg gagcccaagc acctgaagat
     4201 acatgctgaa ctgagcaagt caatgggaac tccagaggag aacatctttg tcatggacac
     4261 tggacagacc ctggaattta caaagaacac agcttcaatt ggaccttcag tgccttcagg
     4321 acacatactg gttgacggcc taggggttgg agatgtagga aacatagtcc ttagggacag
     4381 gaagcacctt tccgaggacg gactcctggt tgtggtaata accatgaaca agaaggaagg
     4441 caaggttgca gcaggaccgg acatcgtatc aaggggtttt gtctatgtaa gggaatccga
     4501 ggaccttatg gaagaggcca agaaggttgt tgcaaagacc ctgaagactt gtgaaagcaa
     4561 gaagatatca gactggtcaa ccataaaatc aaatgtaagg gatgacctta gaagcttcct
     4621 ttacagcaca ataaagagaa atccaatgat acttccaatc atcatggagg tttagaaagg
     4681 gagatcagta aatgggaact agacttttaa tgtaccttgc agtcatactg cttggagcga
     4741 tcataggagc caaggcaaaa cttggggata atctcaaaaa aagactgggc agtattcaaa
     4801 ctgcctgtct tcttttattg ctcctgacca tgggagtaat gattggaatg gacaaggatg
     4861 taatcaattc cttcttctcc attggctact cggcaattgt aatcagcatt tttacagttg
     4921 gcttcagcat tttaggggtg aagcttgtca gtggctatgt gactggaggt gggaagagat
     4981 gaccggaaaa attgttgggg cagtggcagc aggggtcctg cttggactct atgtgctacc
     5041 tgtcaccata cttgatcata ctgacatgat aatagacatt ggacttatgc tcctcctgtt
     5101 tttcgttggg attgaaatcg gcctcaaccg gtctgtcttc acaagaatca ggagcatggg
     5161 gcttaagatt ctactgatac cccttatgat aataacaggg agtgtagtgg gaggaatagt
     5221 tgggggaagc cttcttggga tgccctttaa cgagtcgggg gcagttgggg caggacttgg
     5281 ctggtatacc ctttcatcaa tacttatggc taactactcc aatgagcttt caaccttggc
     5341 atttcttgca aatgttgtaa gggagctaat ggcatttatg ataataccac ttgtggccaa
     5401 aagggtcggc ttcttaagtg caataggacc agggggtgca acctcaatgg atacggcact
     5461 tccactggta tcaaagtcaa cggaccctga aacggcaatc atagcattca tatcaggtgc
     5521 aatctgcacc ttttcagtac ccatccttgt gccgctgatc ttgaatcttt agtaaaagag
     5581 aggaatgaaa acatgaaaat tcgttatatc acaaaggcaa gcctaatagc tgcaatctac
     5641 cttgtgctta tacttattca ggccctgccc tttccagttg caaacctgac cttcggtccg
     5701 gtccagctga ggatagcaga gggcctcacc ctgctgccac ttatggagcc agccgcggta
     5761 ccgggtcttt ttatcggagt ccttttggca aactttctgc tggcgcccta ttcaggcttt
     5821 ggtcttgtgg acatcatagg aggatctctg gttaccctgc ttgctgccta cctgacatca
     5881 aaggcaaaga acaagtacgt gggaatggtg ccgccgattc ttctgaacgg gattatcgta
     5941 tccatatggg tgtcctactt caccagaatt ccctacatct acactgccct gggaataact
     6001 gcgggacagt ttatttcagt tgtggtgttt ggcagcttaa tactctatgt ctatgaaaag
     6061 gcatcaaaat acagggaatg gtagaaggtg tttaaatgag tagtgtcaat gaggactata
     6121 taaccagcta tatcagggaa atcacccccc aacggacagg acagcttaag attcttgaag
     6181 cttatgcaga ggaaaac
//
LOCUS       DASRQF010000111        12400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_393094, whole genome shotgun sequence.
ACCESSION   DASRQF010000111 DASRQF010000000
VERSION     DASRQF010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 12400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12400
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393094"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VFD79_02820"
     CDS             complement(<1..390)
                     /locus_tag="VFD79_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HZH93011.1"
                     /translation="MDKASVDELVNTNDTIYFLKAMDPFSSRRGFLVKSASQIEENKE
                     GLEILQRDGNKYELPGWIDEKIGEEKVKGINISFPHWKDELGDLPSHWRINVIALGDV
                     GSTLLIGLRLLGSNMTIGIYDRSPDKVK"
     gene            complement(453..5177)
                     /locus_tag="VFD79_02825"
     CDS             complement(453..5177)
                     /locus_tag="VFD79_02825"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidyltransferase"
                     /protein_id="HZH93012.1"
                     /translation="MDIGLRLKDYLDSSNRRERIEDIREDRDNSVLSFMEIIHDFLVE
                     FKPVEKNEWIQTLYDYALYKSFPHKWSERIDMDLIALYEEILNLLRYVNGDDHFDYDL
                     SGSIDEFQRFKEAYNKLFIYEIMQLDMVINGYNTIDHVIGVKDVAMHVALQLKELSLP
                     VDLGIVLGASLGHDVGKYGVVKDEQARVPYLHYYYTEQWFKQLRLEKIGHIATNHSTW
                     DLELETLPLESLILIYADFRVKNKEVDGNYAMHTYSLNESFQVILDKLDNVDEAKEMR
                     YRRVYNKLKDFEDYMESMGVDTSLQGTLERQEKIYWSLLRGEDIVDSFKYKAMENNIF
                     LMTNLMNQERFLEILEDARGESNWRILRLYIEVLKEYSTYLTQKQKIMVLSFLTDLLL
                     HKEEDVRKESAELIGKLLANYDEEYRKELPSSIARKKPEETSTVVFKEILESLLLPSH
                     KITDSHNIWLYNLKLIVKSLFETCGIDSYGEYFDALNYFYQRSEELIPVSQFYLVQTV
                     NYIPLEELDENRRSLFYTYILKMVSSDISNIRLSALDRASEVISKVNDGSLYSPMRDY
                     LEARPLKSPIPAENYLKYILSNKLSMDQEIVGNFRENYIQDQANVSELFLENLKTATG
                     WTTKKINIDILYDQVRKNPKETGIHTAIHLCNVLKVSAVERVRNYSGYTLLNIIPMLS
                     LEQRNDVAIELLRALEMENYQFTKFIPEYLGHLFLYLPPQELEEVIEDLEEKIKIARP
                     QTIMLLLNTIGTTIVNYPKYKDLFQEDEVTNMKRLNRLLGLLLVPIASYNVELKTEAL
                     RVIASTVFNSDKLTHHEKQSVFRRIGKKILTLIEDSRENNFVFYNNAASLNHIYRYIS
                     DFEFEHGMLDVTTDEDVVFFPGSFDPFSLGHREIAKEIRRKGFEVYLAVDEFSWSKRT
                     EAHQFRRNIINMSIAKEDGIYLFPKEIPINIGSDRDMVKLREIFGDREIYIAVGSDVI
                     ANASAYRKNKEILDFSHVIFDRRTDQSDTVDFSASDEAISRIRGKVIQLTLPTQYEDI
                     SSTQIRDNIDKNRDISKLIDPMAADYIYNYGLYIREPLYKTLVETKTLDVEVHRNLSS
                     EILIYLMDNFGNQILEEGILELRNKLSYRIILLKDMETGLPLGFSAFYWVRHSMLYDE
                     FKDQDLTQYIRNYAKGRTAFITCLYARDDNDNLMDMVLNETLSIALNRDYNYALYCNT
                     MISGENKKAEEYLLMQGFSQMPFDHKDSPMFMVDMNNPISINLDLENTLKAPYDSDPR
                     IQKVITDTRKKLKRALADLYPNELLISFNRDMVYSKLIQKLCDENGVSILDNRKKSLG
                     EKMCVPFGIILNNSIIPNTVTKTLHTERIFAPDIHSFTVGEFPNYLSLEGQCKTIKSF
                     ERPVMLMDDLLHKGYRIKIVEPLLRKEGIEISKLFVGILSGKGKEIAQIRNLEVEYAY
                     FVPNLRLWFNESSQYPFLGGDMVRKDYLESNLIPSINFILPYVSPRFIKNITQDAIYN
                     LSEICLNNSLDLLRVIEEVYQEINEKNLTLASLGEVIFTPRYPDLERSVLQKRHQKPS
                     TFLEMDIHYLKRLEHTLRGHTR"
     gene            complement(5216..5503)
                     /locus_tag="VFD79_02830"
     CDS             complement(5216..5503)
                     /locus_tag="VFD79_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HZH93013.1"
                     /translation="MMDNINGGSMEKQMELSREDKKPGFIFEYIDQKGVISKISSILA
                     QNDINIASITVTREGEIATMKCDFDHVLDEDIKEELLHSIEFIRTEFLNMD"
     gene            5632..6564
                     /locus_tag="VFD79_02835"
     CDS             5632..6564
                     /locus_tag="VFD79_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HZH93014.1"
                     /translation="MILSIDLSPSVYKWLEINGNSGNAESFNAENTGMIPGDGGSYLS
                     LILRLMNEDSTITGFAGGHTGSFIRSYLEGVGVVDRFVGIGDRNPERLILTFDDREIE
                     INQKNVEINRDEIGLFHRELKEGLNNCEIVCLCGGYPLNMPDDMPTDILHLSREWGRR
                     ILISPGMDALLEVVEAAPFLIVLDIEGMEKLTNLKLQYEGELIRASQYLFQNDIAYVL
                     VDMGENGLLVMNSENGLSMSLAESGLKSKQFMHGGLLAGFAAGMKRKYDLETTARLSF
                     ACGSLDIRSHDGNPDMADIKALMNKIDIRKFHNQ"
     gene            6578..7666
                     /locus_tag="VFD79_02840"
     CDS             6578..7666
                     /locus_tag="VFD79_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZH93015.1"
                     /translation="MVSFIHSGDIHLGMKFNKSSFAGEKGKERREEVWNTFSQLIQYG
                     KSENIDITLITGDLFEGNNLTLKDIKRLKDSFAYAGEQKFIISLGNHDMVFRETVYAE
                     TDWPDNVTILRKTGLQHVQLDALKTTIWAIDAKRDKEAMLNELDNIEPTSGHFNILML
                     HGEFGANVDYPLPDLKFLKKLSMDYIALGHIHKPEFLDHNIAYSGSLEPLDFGERGKR
                     GFIEGKLSDASTFRFIPFSKREFKILEYQLNRESTIEHVKSEIMSLIGEDGKEDLHRI
                     ILEGIAPTLFHKEALETSLRNELYYLELFDKSKPDIDLEGLFDENRNNILGMYIRSFR
                     DEDLENPILKDALYAGVYALLEGSDRPW"
     gene            7660..10032
                     /locus_tag="VFD79_02845"
     CDS             7660..10032
                     /locus_tag="VFD79_02845"
                     /inference="COORDINATES: protein motif:HMM:NF024904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZH93016.1"
                     /translation="MVIRELHLHGFGKFNDYTLKLQSGLNLVHGPNESGKSTLHSFIN
                     GMFYGFKKPYTRRTLYSNDHNRLSPWSGTHYSGSIVFEKEGQLYRIFRVFEKGREETK
                     VYQEETGEDITDRISKGDNSRILQPGEYFFGLNSGIFNNTLFIEQKNITPLGSLAHEV
                     RERLVNASTGGDEKISVEKALSFLDEQLKEIGTIRASTSQYGRLVAEIDKAEKSLMVV
                     EADVAKYKEKAKRRRQILDQVKNLEIDIEKSQTIIGEMERQKKIGKYQEILALRKKNN
                     GLGEELETLLVHDTKDQDDYNEAMKISEEIGLVKSRIGSMEEQLAEIEGRTRRLYGSK
                     NHGKEEFKEILEDGQRFQKLDYRTTGEDDLESLVRELEKQKKRRMSLRLIQMVFVIAY
                     LTSTIYSFSQVNYLLLGIAQTLLMPILYLMVRARTNNGQIDKLETGVNLELEKDSILT
                     KYKLSNGFEFYEMLEKAKVDKMKAEQEAELMAELNESRECLTARVSSLGTELLALEKG
                     LEGLLSKNHAHDMNSFRQGLEKKLRLREIKREIEYNQETIMRILEGESIEELARLHND
                     YSNGKKDMNSSPLNEVAEKHQMLIQRKSDLLLAVNQLDGSLSQLESSLETEMILQEKI
                     QALIVKKSYLTKRKESLELARARIKALSGEIHREFAPSLNKRVGDFIGRITSGRYSDV
                     KIDRNLGFNLVQSDGNRLIPLENLSGGTLDQMYFGLRMGIVDELISSNLPIFLDECFS
                     QYDDTRLENILNYIASFENRQIIIFTCQMREEEALERLGKSYNKINLQFS"
     gene            10046..10732
                     /locus_tag="VFD79_02850"
     CDS             10046..10732
                     /locus_tag="VFD79_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZH93017.1"
                     /translation="MIYAIGDLHFDHSEQKPMDIFGENWINHKEKIVQDWKSKVCDQD
                     LVLVPGDISWALKLEEAIPDLELIDSLPGHKLFIKGNHDYWWESISRLKALNLESITF
                     IRNSSFIYKDVGIGGTRGWMSRDSDGFDEGDEKIYRRELLRLDASLSSIDKGVSKKIA
                     MLHYPPFDGKLRPNDFVEVLKKHEVDICIYGHLHAEGHRYSVEGLIQGIDFKMVACDF
                     LDFKLKRIDF"
     gene            10757..11494
                     /gene="nagB"
                     /locus_tag="VFD79_02855"
     CDS             10757..11494
                     /gene="nagB"
                     /locus_tag="VFD79_02855"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005398467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HZH93018.1"
                     /translation="MKIIVTESYKEMSRVAAELFEEEILKNPKVVLGLATGSTPIGTY
                     GELVRKHREEKLDFSQVTTFNLDEYVGLDDENPNSYRFFMEKHLFDKVNINKMNCHVP
                     DGKTDNPDEYGKKYDNMIEAAGGIRIQLLGIGSNGHIAFNEPGDYLPVGTGLVNLTRQ
                     TVEDNARFFQSIDHVPTQAISMGMGSIMKAEKIVILANGKGKAEIIKKFVEDARVSTR
                     IPVSFLLLHPDVTLVCDKEAYSLVADS"
     gene            11505..12071
                     /locus_tag="VFD79_02860"
     CDS             11505..12071
                     /locus_tag="VFD79_02860"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HZH93019.1"
                     /translation="MVKELEVKVLGIDIDHVQDLIIAKGGELIGKEKQINTLIDSKDN
                     PIKSYMDAYLRIRENHDLLGDKRTVTFTLKKNLPNKSLRENEEYNVLVDDRDTLLRIM
                     ENLGFDKSTVGYKDRTSYSFLNGRIDIDIWDEATYPYPYMEIEVQNEQELDIILKELE
                     IPQEKVSKLSIVELQEKLVNRRDDKYVE"
     gene            12061..>12400
                     /locus_tag="VFD79_02865"
     CDS             12061..>12400
                     /locus_tag="VFD79_02865"
                     /inference="COORDINATES: protein motif:HMM:NF013673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HZH93020.1"
                     /translation="MLNRKIVENVLEEALSRGGDFAEIFLEDKMTSNIKLVQGEIKDS
                     LSGRDFGVGIRILDGTNSVYGFTNGSSEKELVNLAKSLALSMDGGSKAHSRMLGEAII
                     HDNSPVKTQLS"
BASE COUNT         3832 a         2275 c         2725 g         3568 t
ORIGIN      
        1 cttaaccttg tctggagatc tatcatagat tcctatggtc atgttggaac ctaggagcct
       61 caatcctatg agtagtgtac tacccacatc ccccagagct atgacattga ttctccagtg
      121 ggagggaagg tcaccaagct catccttcca gtggggaaag gagatgttta tccccttaac
      181 cttttcttct cctatctttt catcaatcca gccaggcaat tcatatttgt tcccgtctct
      241 ttgaagaatt tcaagaccct ccttgttttc ttcaatttgg gatgctgatt taaccaaaaa
      301 ccccctcctg gaagaaaagg ggtccatggc tttaagaaag taaatcgtgt catttgtatt
      361 gactagttca tctacacttg ctttatccat atcttcaata tggattttgg aaaacattag
      421 tttgttatta tatttatagt aatacatttc aatcacctcg tatgtcccct aagagtatgt
      481 tccagtctct ttaggtagtg gatgtccatc tccaggaatg ttgatggctt ttgatgcctt
      541 ttttgaagaa cacttctttc aaggtcagga tacctaggtg tgaagattac ctcgccaaga
      601 gaggcaaggg tgaggttctt ctcgttaatc tcctgatata cctcctcaat gacccttaat
      661 aggtccaatg aattattcag acatatttca gatagattat aaattgcatc ctgagtaata
      721 ttctttatga atctagggga cacgtaagga agtataaaat taatgctagg aatcagattt
      781 gattcaagat aatccttacg taccatgtca ccgcccagaa aggggtattg gctactttcg
      841 ttgaaccaca atctcaagtt gggaacaaaa taagcatatt ccacttcgag gtttcttatt
      901 tgggcgattt ctttgccttt tcctgacagg attccaacaa agagcttgga aatctcaatg
      961 ccttcctttc taagcagagg ttcaacaatc tttattctgt aacccttgtg aagaagatca
     1021 tccataagca ttactggccg ttcaaatgac tttatggttt tacactggcc ctcaagagac
     1081 agatagttgg gaaattctcc aactgtgaag ctgtgtatgt ccggcgcgaa tatcctttca
     1141 gtatgaagtg tctttgttac agtatttgga attattgagt tgttcaatat tataccaaag
     1201 ggcacgcaca tcttttcgcc cagcgacttc ttcctgttgt ccaagatcga tactccattt
     1261 tcatcacaaa gcttctggat gagcttgctg taaaccatgt ccctgttgaa tgaaatgaga
     1321 agctcattgg gatagaggtc cgccagggct ctcttaagtt tctttctggt gtctgtaatg
     1381 accttttgaa ttcttggatc gctatcatag ggtgccttca gagtattttc cagatcgaga
     1441 ttaatactta tgggattatt catatcaacc ataaacattg gtgaatcctt gtgatcaaat
     1501 ggcatctggg agaagccctg cattagcaag tactcttcgg cctttttgtt ctcacctgaa
     1561 atcatggtgt tacaatagag ggcatagttg tagtccctat tcaaagcaat ggacaaagtt
     1621 tcgttgagaa ccatatccat aaggttgtcg ttatcatccc ttgcataaag gcaggttatg
     1681 aatgctgttc tccccttggc gtagtttctg atatattggg ttaggtcctg gtctttgaat
     1741 tcatcataaa gcatactgtg cctgacccaa tagaaggctg aaaaacctag tggtagaccg
     1801 gtctccatat cctttagaag tattatccta tagcttagct tgtttctaag ttcaagtatt
     1861 ccctcttcca gtatctgatt gccaaagttg tccataaggt atatcagtat ttcactactt
     1921 aggtttctgt gaacctccac atccaatgtt ttagtctcaa ccagggtttt gtaaagaggt
     1981 tctctaatat aaagaccata attgtatata tagtcagcag ccattgggtc aataagtttt
     2041 gaaatatccc ggttcttatc tatattatcc cttatctgag ttgagcttat atcctcatac
     2101 tgggttggga gtgtaagctg gatcaccttg cctcttattc tggaaatggc ttcatctgaa
     2161 gcggaaaaat caaccgtatc agactgatct gtcctcctat cgaagataac atgtgaaaaa
     2221 tccagaattt ccttgttttt cctgtacgct gatgcattgg caataacatc tgagccaacg
     2281 gctatatata tttccctgtc cccaaaaatt tcccttagtt taaccatatc ccgatctgaa
     2341 ccgatgttta ttggaatttc ctttgggaag aggtagatgc catcctcctt ggctatactc
     2401 atatttatga tattacgtct gaattgatgg gcttctgttc gctttgacca tgaaaactca
     2461 tccacagcaa ggtatacctc aaagcccttt cttcttattt cctttgcaat ctccctgtgg
     2521 ccaagactga atgggtcaaa ggagccaggg aagaagacaa catcttcatc tgttgttaca
     2581 tccagcatcc catgctcaaa ttcaaaatcg gatatatacc tgtatatatg atttaaagaa
     2641 gctgcattgt tgtagaagac gaaattgttt tcacgtgaat cctcgattag agtaagtatt
     2701 ttctttccaa tgcgtctaaa tactgattgc ttctcatggt gggtaagctt gtcagaatta
     2761 aaaaccgttg aagcgataac cctaagggct tctgtcttaa gttcgacatt ataggatgca
     2821 atgggaacaa gtaacagtcc caacagccta ttaagacgct tcatattagt cacttcatcc
     2881 tcctggaaca aatccttata tttgggataa tttactattg ttgtaccaat ggtattgagc
     2941 aatagcatta tggtttgtgg ccttgcaatc ttgattttct cctcaaggtc ctcaattacc
     3001 tcctccagtt cctgaggcgg taggtatagg aaaagatgtc ccaaatactc aggtatgaac
     3061 ttggtgaact ggtaattctc catttccaga gccctaagaa gctctattgc aacatcattt
     3121 cgctgttcca gtgagagcat aggaataatg tttagcagag tatatccgct gtaatttcta
     3181 accctttcca cagcactgac ctttagcacg ttgcaaagat gtatggctgt gtgaatacca
     3241 gtctctttgg gatttttacg cacctgatcg tataggatat caatgtttat cttctttgtt
     3301 gtccaacctg ttgcagtctt aaggttctcc agaaacaatt cggacacatt ggcctggtcc
     3361 tggatatagt tttccctgaa gttaccaact atctcctggt ccattgatag cttgtttgaa
     3421 aggatatact taaggtagtt ttcagcaggg atgggggact taagcggcct ggcctcaaga
     3481 taatctctca taggggagta aaggcttccg tcatttacct tgcttattac ctcagatgcc
     3541 ctgtcaagcg cactaagtct gatgtttgag atatcagaag aaaccatctt taggatgtag
     3601 gtgtagaaga gacttcttct attttcgtcc aactcctcca agggaatgta gttaactgtt
     3661 tgaacaagat agaactgaga aacagggatt aactcctctg aacgttgata gaagtagttc
     3721 aaggcatcaa aatactcccc gtagctatct atgccacagg tttcaaacaa tgactttaca
     3781 atcagcttaa ggttgtacag ccagatattg tgagaatcag tgattttatg gctgggtaac
     3841 aagagagatt caagtatctc cttgaataca accgtgctgg tctcctcagg tttttttcgt
     3901 gctattgagg aagggagttc ctttctgtac tcctcatcgt agtttgccag gagtttacct
     3961 atcaattcgg ctgattcctt tcggacatcc tcctccttgt gcagaaggag gtctgttaga
     4021 aagcttaaga ccattatttt ctgtttctgg gtaagatagg tcgagtactc cttaagaacc
     4081 tcaatatata gcctcaaaat cctccaattt gattctcccc ttgcatcctc caggatttca
     4141 aggaatcttt cctgattcat aaggtttgtc ataaggaata tattgttttc catggccttg
     4201 tacttaaagg aatctacaat atcttcacct ctaagcaggc tccagtagat tttttcctgt
     4261 ctttccagag ttccctgaag gcttgtatcc acccccattg actccatata atcctcaaaa
     4321 tccttcagct tgttgtagac cctcctatat ctcatctctt tggcttcatc aacattgtct
     4381 agcttgtcca gaataacctg gaaggattca ttcaggctat atgtatgcat ggcataattg
     4441 ccgtccactt ctttattctt aaccctaaag tctgcataga tcaggatcag agactccaag
     4501 ggaagtgttt ccagctccag gtcccatgtt gaatgattcg tcgcaatgtg gccaattttc
     4561 tccagtctca gctgtttaaa ccattgctcg gtgtagtagt agtgaaggta gggtaccctg
     4621 gcttgctcat cctttaccac gccatacttt ccaacatcat gaccaaggct ggcgccaaga
     4681 actattccaa ggtcaacagg aagactcaac tccttaagct gcagtgcaac atgcatggcc
     4741 acatccttga ctcctatgac gtgatcaatt gtgttgtagc cattgatgac catgtccagc
     4801 tgcatgattt cataaatgaa aagcttgttg taggcctcct taaaacgttg gaactcatct
     4861 atggatccgg ataggtcata atcaaaatga tcatcgccat taacatatct aagtagattt
     4921 agaatctctt cgtaaagtgc aattaaatcc atatcaatcc tttcactcca cttgtgtgga
     4981 aaggacttat acaaagcgta gtcataaagt gtttgaatcc actcattttt ttcaacaggt
     5041 ttaaattcta caaggaaatc atgaatgatc tccataaaag aaaggacaga attatctctg
     5101 tcttccctta tatcttctat tctttctctc ctgttgctgc tatcaagata atcctttaaa
     5161 cgtagcccaa tatccatggt cttcctcctc gttgagtata aatggaatgt aaatcctaat
     5221 ccatgtttag aaattcagtc cttataaatt caatggagtg aaggagctct tcctttatat
     5281 cctcatccag aacatggtca aaatcacatt tcattgttgc aatctcacct tctcgtgtta
     5341 ctgtaatact ggctatatta atatcattct gggcgagaat agagcttatt ttacttatta
     5401 cacccttttg gtcaatgtat tcaaaaataa aaccaggctt cttgtcttct cttgacaatt
     5461 ccatctgctt ttccatggaa cccccattaa tattatccat catagaatac ccctcctttg
     5521 gttacttcaa ttatataatt aagagtcctg cttttcaatg atgaattggt aaagacaatt
     5581 gcaagataca ggcatataag ggtataatag aaagcgatgg gggtggagga aatgatacta
     5641 tccatagatt taagtccttc agtatacaaa tggctggaaa tcaatgggaa ttcaggaaat
     5701 gctgaatcct tcaatgcaga aaacacaggt atgataccag gggatggagg ttcatatctt
     5761 tccttgattc taaggttgat gaatgaggat tctacaatta caggcttcgc aggaggacac
     5821 actggaagct tcatacgaag ctacctggaa ggggtcggag tagttgatag atttgttggg
     5881 ataggagata ggaatcctga gaggttaatt ttaacctttg atgataggga aattgaaatc
     5941 aaccaaaaaa atgtagaaat aaacagagat gaaataggtc tttttcacag ggagcttaag
     6001 gagggcctta acaactgtga aatagtatgc ctgtgcggtg gctacccctt gaatatgcca
     6061 gacgacatgc caacagacat cctgcaccta tccagagagt ggggtaggag aatcctgata
     6121 tcaccaggca tggacgcatt gttggaggtt gttgaagctg caccatttct gatagttttg
     6181 gatattgaag gcatggaaaa attaacaaat cttaagcttc aatacgaagg tgaattaata
     6241 agggcaagcc aatacctttt tcagaatgat attgcatatg tacttgtaga catgggagaa
     6301 aatgggttgc tggtcatgaa tagcgaaaat ggactcagca tgagtctggc cgagagtggc
     6361 ttgaagtcaa aacagtttat gcatggtggt ctattggcag gcttcgctgc tggaatgaag
     6421 agaaaatatg atttggagac aacagcaagg ttatcatttg catgtggttc acttgatatc
     6481 aggagtcatg atgggaaccc tgatatggct gatatcaagg ctctgatgaa caagattgac
     6541 ataaggaagt ttcataatca ataggaatgg tgatatgatg gttagcttta tacattctgg
     6601 agatattcat ctgggaatga aatttaataa atcaagcttc gcaggggaga agggcaagga
     6661 aagaagagag gaagtctgga atacctttag ccaattgatt cagtacggga aaagcgagaa
     6721 cattgatata acactcataa caggagacct ttttgaaggg aataatctta cccttaagga
     6781 tatcaagagg ctcaaggact cctttgcata cgcaggggaa cagaaattca taatatccct
     6841 gggaaatcac gatatggtat ttagggaaac ggtttatgca gaaactgact ggcctgataa
     6901 tgtaacaata ctgagaaaaa cgggtcttca gcatgttcaa cttgacgctt tgaaaacaac
     6961 aatctgggca attgatgcca agagggacaa ggaagccatg ttaaatgagc ttgacaatat
     7021 agagcccacc agtggacact ttaacattct aatgctccat ggtgagtttg gggcaaatgt
     7081 ggactatcca cttcctgatt taaagttttt aaagaaatta tccatggatt acatagccct
     7141 tgggcatata cataaacctg agtttttgga ccataacata gcatattcag gttctttgga
     7201 gcccttggat tttggagaaa ggggaaagcg gggcttcatt gagggcaagc tttctgatgc
     7261 cagcactttc agattcattc cattttctaa gagagaattt aagatacttg agtatcaact
     7321 gaatagggaa tccacaattg agcatgtaaa atctgaaatc atgagtttga ttggcgagga
     7381 tgggaaggag gaccttcaca ggataatcct agaagggata gctccgacac tttttcacaa
     7441 ggaggccctg gagacttcct tgagaaatga gctctactat cttgagctat ttgacaaatc
     7501 aaagccagat attgatcttg aaggattgtt tgatgaaaat agaaacaata ttctagggat
     7561 gtacataagg agcttcaggg atgaggacct ggagaaccct attctaaagg atgcattgta
     7621 tgcgggagtc tatgctcttt tggaaggaag tgaccggcca tggtaattcg ggaactccat
     7681 cttcatggat ttggcaagtt caatgactac accctgaaac tccagtcagg actcaatcta
     7741 gttcatggtc ccaatgaaag tggtaaatca acccttcaca gctttataaa cggtatgttt
     7801 tatgggttta aaaaaccata cacaaggagg acactttatt ccaacgacca caacaggcta
     7861 tccccttggt caggaacaca ttacagtggc agcatcgtat ttgagaagga aggtcaactg
     7921 tataggatat tcagggtctt tgaaaagggc agagaagaaa caaaggttta ccaggaagag
     7981 acaggtgagg atataacaga caggatctcc aagggagata attcaaggat tttacagcct
     8041 ggtgagtact tctttggttt gaacagtgga atattcaaca atacactttt tattgagcag
     8101 aagaacataa cacctttagg ctcactggca catgaagtca gggaaagatt ggttaatgca
     8161 tcaactggtg gagatgagaa aatctcagtg gagaaggccc tttcgttttt ggatgagcaa
     8221 ctgaaagaaa ttggtactat tagggcatcc acatcacaat acggtaggct tgtggcagaa
     8281 atcgacaaag ctgaaaaaag cttaatggtt gttgaagcgg atgttgcaaa gtataaggaa
     8341 aaggcaaaga ggagaagaca gattcttgac caggtgaaaa accttgagat tgacattgag
     8401 aagagtcaaa ctatcattgg tgaaatggaa cggcaaaaaa agataggaaa atatcaagaa
     8461 atcctggcct tgaggaagaa aaacaatggg ctgggtgaag aactggagac attgctggtt
     8521 catgatacaa aggaccagga tgattacaat gaagcaatga aaataagtga agagattggt
     8581 cttgtgaagt caaggatagg gtccatggag gagcaacttg cagaaattga aggaaggacc
     8641 agaagattgt atggaagtaa gaatcatgga aaagaggaat tcaaggagat actggaagat
     8701 ggacaaaggt ttcagaaatt ggactaccgg accactgggg aggatgacct ggaatctttg
     8761 gtaagagaat tggaaaagca aaaaaagaga cgaatgtcat taagactgat tcaaatggta
     8821 tttgttattg catatttaac ctcaaccatt tattcattta gtcaagtcaa ttatttgctt
     8881 ctaggcattg cacaaacatt actgatgcca attttatatc ttatggtaag ggcacgaacc
     8941 aataatggac agattgataa attggagact ggagtaaatc ttgagttgga aaaggatagc
     9001 atattgacaa agtataaatt atccaatgga ttcgaatttt atgaaatgct tgaaaaagcc
     9061 aaggtagata agatgaaagc cgagcaggag gctgaactca tggcagaact caatgaatca
     9121 agggaatgct taaccgcaag ggttagtagc cttggcacag agttgcttgc attggaaaag
     9181 gggctggagg gcttgttgag caagaatcat gcccatgata tgaactcctt tagacaaggt
     9241 ttggagaaaa aacttaggtt aagggagatc aagagggaga ttgaatacaa ccaggagacc
     9301 ataatgagaa ttcttgaggg agaaagtatt gaggagcttg ctaggctaca caatgattac
     9361 tcaaatggta aaaaggacat gaactcaagc cccttgaatg aagttgctga aaaacaccaa
     9421 atgttgattc agaggaaaag cgaccttctt ttggctgtaa atcagttgga tggttcattg
     9481 tcacagctag aaagctcctt ggaaacagag atgatattac aggaaaagat acaggcactg
     9541 attgtcaaga aatcctacct cacgaagagg aaagagtcac ttgaattggc tagagctagg
     9601 atcaaagccc tatcaggtga aatacacagg gaatttgcac cttccttaaa taagagggtt
     9661 ggggatttca ttggcaggat tacatcaggt agatattcag acgtgaagat agacagaaat
     9721 cttggtttta atctggtcca atcagatgga aacaggctaa ttcccttgga aaacctaagc
     9781 ggtggaacat tggaccaaat gtacttcgga ttgagaatgg gtattgttga tgagttgatt
     9841 tcaagtaatt taccaatatt tctggatgaa tgcttttcac agtatgatga tacgagactt
     9901 gaaaatatac tgaattatat agcaagtttt gaaaataggc agattataat atttacctgc
     9961 cagatgaggg aggaagaagc cctggagagg ttaggcaaaa gttacaacaa gattaacctg
    10021 caatttagct aagagaggtg cgtatatgat ttatgcaata ggtgatcttc acttcgatca
    10081 ttccgaacag aagcctatgg atatttttgg agagaactgg ataaaccaca aggagaagat
    10141 tgtccaggat tggaaatcaa aggtatgcga ccaggatctg gttctagtac cgggagatat
    10201 ttcttgggcc ctaaaacttg aagaggccat accggatctt gagctgatag acagcctccc
    10261 ggggcataag ctatttatca agggcaatca tgactattgg tgggaaagca tttctagact
    10321 taaggctttg aatctggagt ccataacatt tataaggaac agttccttta tctacaaaga
    10381 cgtggggatt gggggcacaa ggggatggat gagcagagac tcagatggct ttgacgaagg
    10441 agatgaaaag atatatagga gggagctact aaggcttgat gcatccctat catcaataga
    10501 taagggagtg tccaaaaaga tagccatgct tcactatcct ccctttgatg ggaagttaag
    10561 acccaatgac tttgtggaag ttctgaagaa acatgaagtg gacatatgca tttatggtca
    10621 tttgcacgct gagggacaca ggtattccgt tgaagggctg atccaaggga tagactttaa
    10681 gatggtagcc tgtgatttct tggatttcaa acttaagagg attgattttt gaaaatatta
    10741 tacaaggggg actggcatga aaattattgt gactgagtca tacaaagaaa tgagcagggt
    10801 ggctgctgaa ctatttgagg aagagatatt gaagaatccc aaggttgtcc ttggcctggc
    10861 aactggatca actcctattg ggacttacgg ggagctggta agaaagcata gggaggagaa
    10921 actggatttc tctcaggtca ccacattcaa tctggatgag tatgtaggtc ttgatgatga
    10981 gaatccaaac agctacaggt tctttatgga aaaacaccta tttgacaagg tgaatataaa
    11041 caagatgaat tgccatgtcc cagatggaaa aacagacaat ccagatgaat acggtaaaaa
    11101 gtatgacaat atgattgaag ctgcgggagg tataagaatt caattgcttg ggataggatc
    11161 aaatggtcac atagccttta atgaaccagg tgattattta cctgttggaa caggattggt
    11221 aaatttaacc agacaaaccg tagaggacaa tgccagattc ttccagtcaa tagaccatgt
    11281 tcctacccag gcgatttcaa tgggaatggg atctataatg aaagctgaaa agatcgtcat
    11341 acttgccaac ggaaagggaa aggcagaaat cataaaaaaa tttgttgaag atgctagggt
    11401 aagcactcga attccagtat catttttgct tcttcatccc gacgtaacct tggtttgcga
    11461 taaggaagca tattcattgg tagcagatag ttaggaggaa gcttatggta aaggagttgg
    11521 aagttaaggt tttgggaata gatattgacc atgtccaaga tttgattata gccaagggtg
    11581 gagaactgat tggcaaggaa aaacaaatca acacactgat cgattccaaa gataacccaa
    11641 taaaatcata catggatgca tatttgagaa tcagggaaaa ccacgatctc cttggggata
    11701 aaagaacagt gacattcact cttaagaaga atcttccaaa taagagttta agagagaatg
    11761 aagagtacaa tgtattggtc gatgacaggg atacacttct tagaatcatg gaaaatctgg
    11821 gttttgacaa gtccacagta ggttacaagg acagaacatc ctattccttc ttaaacggta
    11881 gaattgacat tgacatatgg gatgaagcaa cctaccccta cccatacatg gagattgagg
    11941 tccaaaacga acaggaactt gatattattt taaaggagct tgagataccc caggagaaag
    12001 tatccaagct ttcaatagtt gagcttcagg agaagttagt gaataggcgg gatgataaat
    12061 atgttgaata ggaagatagt cgagaatgta cttgaagaag ccctatcaag aggtggagat
    12121 tttgcagaaa tctttcttga agacaaaatg acttcaaata taaaattggt tcagggtgaa
    12181 ataaaagata gcctttcagg aagagacttt ggggttggca taaggatact ggatggaaca
    12241 aatagtgtct atggcttcac aaacggctcc tctgagaagg agcttgttaa ccttgcaaaa
    12301 tctttggcat tgtcaatgga tggaggctca aaagctcatt ccagaatgtt gggagaagca
    12361 attatccacg acaatagccc agttaagact cagttatcaa
//
LOCUS       DASRQF010000112         6021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_657358, whole genome shotgun sequence.
ACCESSION   DASRQF010000112 DASRQF010000000
VERSION     DASRQF010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657358"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..1122)
                     /locus_tag="VFD79_02870"
     CDS             complement(217..1122)
                     /locus_tag="VFD79_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HZH93021.1"
                     /translation="MHKLSGDKVIYTFRTEMEPVLTMKPGEEVLVETNDCFYQQIQSE
                     DQVMMEIDFDQINPATGPIYVEGAEPGDLLKVEILEIKVADKGVSGVVPGEGILGDKT
                     KKPIVRIIPIQDGHAHFKGLKLPIKPMIGVIGVAPSKDDGDCPTETPWKHGGNMDTTD
                     IAQGNTVYFPVNQKGALLALGDCHAIMGDGEICFTGLEVPARVRLRVDVIKDRKVNWP
                     LIGTKNSTMVVASGDDVDRAIYNATDETVDLLSRALRMDFEEAYLLTSIGVHMRISQV
                     VDPRKTIRSEIPHSIITTEKLLENI"
     gene            complement(1150..1647)
                     /locus_tag="VFD79_02875"
     CDS             complement(1150..1647)
                     /locus_tag="VFD79_02875"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZH93022.1"
                     /translation="MSRDVYLDAIQNVECSFAYHKAIFDDEGKMIDYMFLDVDDFKDI
                     NDQYGHRAGDKVLQYLAMRVKQELREEDLIVRTGGDEFLLYLHDIRDEDNARIFVDRI
                     YKKITGSYRLKDDFNEDPQNLEVSLSIGVAFYPKDGDDIFQLMSKADQALYRVKRSGK
                     SDYHM"
     gene            complement(1902..3179)
                     /locus_tag="VFD79_02880"
     CDS             complement(1902..3179)
                     /locus_tag="VFD79_02880"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HZH93023.1"
                     /translation="MKLKKIYYALPIFILAMVMVGFVATQPSNSLTMDVNPSIEIVTN
                     RLDRVVEINPLNKDADDLLREFNPKNKNLEDTVNDLVDLMILTGHIKGGEDNFVMITV
                     DDDSMDSNLVRKVNRAIAAMLENKQIEATVLNQAIARSEDGRKLTGVELAASRLDKLD
                     DDMDGETLANMTVRQLIHYSKDQNIPIESLFRVAAGTIDNTLESRVMISREEAERIAL
                     GKINGEIIKFELDDLHDDDGPEYEIEILAKDGKYEIEIDAFTGVIKEFERDDHDSKED
                     RTQAAGSFISPEEARKIALGLVKGEIIKLELDDEDDDPEYEIKVRKDGVIYEIELDAR
                     SGRVEEFERDDDDDKRAPAPKDKPASSTNSRISPDQAKSIALEMTGGGRITEFEWDDD
                     EYEIEIIKDGMEYEIEIHAYTGRVLDFEVEDDD"
     gene            complement(3260..4354)
                     /locus_tag="VFD79_02885"
     CDS             complement(3260..4354)
                     /locus_tag="VFD79_02885"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HZH93024.1"
                     /translation="MKDKQILDSVKRSMNQAPIDILDNIKSTPRTRMLRHDEITKQQS
                     GPYNYRRYLPYASLAAAFVLVFLGWQLGTRMPDSRIYLDVNPSIQMTTNRQDKVIGLV
                     AENGDGQGLTRDLDYKGMTVYEVTEEILTRMMDERYLDKDHEFLLLSVYNQDQDKAER
                     QKRELDTRIHQYLQAKDLQPVVLSQKLENTSTIESYAREYSISVSKMTFIRNLIILNP
                     ELQVEDLAGLSIEDLVRLSQGMNLDLERIIDSNDFDRIREEVPQPQPETPPKPVGYDD
                     DDDDDDDDDDDDDDDDDDDDTPQPTIQSMISPDQARSIALSVANGVITDFDFDDDDME
                     YEVEIELGDLEYEITIDARTGAVIEVEIDD"
     gene            complement(4351..4941)
                     /locus_tag="VFD79_02890"
     CDS             complement(4351..4941)
                     /locus_tag="VFD79_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZH93025.1"
                     /translation="MILFTIVYDDNPNDKGHKRIEIDEGLFRRLAKDDMSALDDLYAL
                     TERTMYAYSVSLTRDHQMALDLMQDTYVKILSAAHLYKPMGKPLAWMFTIAKNLHYSN
                     TKKEKRSISMEPEEVADDRRFSYVTDMDDRIVLEGVLTLLTQEEREIVMLHAVSGMKH
                     KEIAEGLGLNLSTTLSKYHRALKKLREYLEGKEARA"
     gene            complement(5187..>6021)
                     /locus_tag="VFD79_02895"
     CDS             complement(5187..>6021)
                     /locus_tag="VFD79_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93026.1"
                     /translation="LLIFAMIGTGTWVYADDYELNALETDGTEFMIKVNRRGKVTSVE
                     RDGEEISFENSDIKDFLEELFDKYDIEAIAPMGDSEEDAEKLAEEIRAMIEERREEAE
                     KKAEDAREKAEEARERAQEEKGDDDYDNEGLRAREENMSVKGLSPEDVERARELGITP
                     GKYNIITNLMEVENVDRYLNRIRSEYGIDEDEDTDNKELSKRIMEEFSARNGEKNNPS
                     VNAPGQADKDEKDERDNPSQFAPGQVKKLEANDNDEDAIDDEDEENGELGRPASPGKS
                     N"
BASE COUNT         1309 a         1550 c         1101 g         2061 t
ORIGIN      
        1 tgctgttgta cttcttggca acatcaattg ccttttctgc tgcatgctca gatgtaggtg
       61 agccatcaat gggaaccaga atcttatcca taatactacc tcctatccaa attactacct
      121 tacttcctac cacatatata ccctaagaat tttttgttaa aagtatccca ttgatatagg
      181 gaaaaatata gcctgtgcag ggcacaggct aagtggttag atgttttcaa gcagtttttc
      241 agtcgttatt atagaatgag ggatttctga ccttatggtc tttctagggt cgacaacctg
      301 ggaaatcctc atatgaaccc ctatgcttgt aagaagatag gcttcctcaa agtccatacg
      361 gagagccctt gaaagcaggt ccacagtttc atccgttgca ttgtagatag ccctgtcaac
      421 atcatctccc gaagcaacca ccatggttga gttttttgtc cctatcaggg gccagttgac
      481 cttcctgtcc tttataacat caaccctaag cctgaccctt gctggaacct caaggcctgt
      541 aaagcagatc tcaccatccc ccatgatcgc atggcagtct ccaagggcca gaagggctcc
      601 cttctggttg acgggaaagt atactgtgtt accctgggct atgtctgttg tatccatgtt
      661 tcccccatgc ttccatggtg tttcagttgg gcagtcccca tcatcctttg aaggtgcaac
      721 tccgatgaca cctatcattg gctttatggg aagcttcaat cccttgaagt gggcatggcc
      781 atcctggatt ggtattatcc taactattgg ctttttggtt ttgtccccaa ggattccctc
      841 tccaggtaca actccagaga cacccttgtc tgcaaccttt atctccagaa tctccacctt
      901 cagaaggtct cctggctctg ccccctccac atagatgggg cctgttgccg gattgatctg
      961 gtcaaagtca atttccatca ttacctggtc ctcactttgg atctgctggt agaagcagtc
     1021 gtttgtctcc acaaggacct cctctccagg cttcattgta agtacaggct ccatttccgt
     1081 cctaaaggtg taaatcacct tgtctcctga cagtttatgc attttacccc tcctctttta
     1141 aatctagaat cacatatgat aatccgactt gccgcttctc ttgaccctat acagggcttg
     1201 gtctgccttg gacatcaact ggaatatgtc atccccatcc tttggataga atgcaacccc
     1261 tatgcttaag ctgacttcca ggttttgcgg gtcctcattg aagtcgtcct ttagcctgta
     1321 gcttcccgtt attttcttgt atattctatc gacgaatatt cttgcattat cctcatccct
     1381 aatgtcgtga agatacagga gaaactcgtc cccacctgtc cttacgatca ggtcctcttc
     1441 ccttagttcc tgctttaccc tcatggccag atactggagg accttatcac cggccctgtg
     1501 tccgtactgg tcattgatat ccttaaagtc gtccacatcc aggaacatgt agtctatcat
     1561 tttcccctca tcatcaaata ttgccttgtg atatgcaaat gaacactcta cattttgtat
     1621 ggcatctaag tacacatccc tactcatggg attcctcccc tccttctgac aatctcatat
     1681 ctatcttcat accatttctg gtaatttaag cctacatgat aattctacca taaaagacac
     1741 tatcccaaaa tccttttctt tccattcttg cctttccctg ggcttctatg aagatggatt
     1801 catacttcac atttggtgga taggtggcca ctctcctaaa aactattata ctagatgggc
     1861 atggaaaaag ggaggatttc tcctcccctc aagtctattg attaatcgtc atcctctacc
     1921 tcaaagtcca ggacccttcc agtatatgca tggatctcta tttcgtattc cattccatcc
     1981 ttgatgattt ctatttcgta ctcatcatcg tcccattcaa actctgtgat tcttcctccc
     2041 cctgtcattt caagggcaat ggattttgcc tggtcaggtg atattcttga atttgttgaa
     2101 cttgcaggct tatcttttgg cgcaggagct cttttatcat cgtcgtcatc tctttcaaac
     2161 tcctccaccc ttccagacct ggcatccagt tcgatttcat agataactcc atcttttctt
     2221 accttgatct catactcggg atcatcatct tcatcatcaa gctccagctt tatgatttca
     2281 ccttttacaa ggccaagagc aatttttcta gcctcctcag ggctaatgaa gcttcccgcc
     2341 gcctgggttc tgtcttcttt actgtcatgg tcgtctcgct caaattcttt tattacccct
     2401 gtgaatgcat ctatttctat ctcatacttg ccatcctttg ctagaatctc gatttcatac
     2461 tcaggtccgt catcgtcatg gaggtcgtcc agttcaaatt tgattatctc tccgttaatt
     2521 ttcccaagag caattctctc tgcttcttcc cttgaaatca ttactcttga ttccaaggta
     2581 ttatcgatgg ttccagctgc aaccctaaag aggctttcaa ttggaatgtt ctggtccttg
     2641 ctgtagtgga taagctgtct aactgtcatg tttgcaagtg tttctccatc catgtcatcg
     2701 tcaagtttat ccagtctgct tgctgccagc tcaactcccg taagctttct gccgtcttca
     2761 cttctggcaa ttgcctggtt caatacggtt gcctcgattt gcttgttctc cagcattgct
     2821 gcaatggccc tgtttacctt ccttaccaga ttgctgtcca ttgagtcatc atcaactgtt
     2881 atcattacaa agttgtcctc tccacccttt atatggcctg ttagtatcat aaggtcaacc
     2941 aggtcgttga cagtatcctc caggtttttg ttcttggggt tgaactccct aagaaggtcg
     3001 tctgcatcct tgtttagtgg attgatctcc accaccctgt ccagcctgtt ggtcactatc
     3061 tcaatgcttg gattaacgtc cattgtcagt gagttggatg gttgggtggc tacaaaaccc
     3121 accattacca ttgccagtat aaatatcggg agtgcgtagt aaatcttttt aagtttcatt
     3181 tccatatcct cctttttctt ttctatactg ggttaactcc tggggtcttc attttattgc
     3241 atggatttca aaagtttttt taatcgtcga tttcaacttc gatgaccgca ccggttctgg
     3301 catctattgt tatctcatac tcaaggtctc ccagctcgat ttcaacctca tactccatat
     3361 cgtcatcatc aaaatcaaag tctgttatga ccccatttgc aactgacaat gcaatggacc
     3421 ttgcctggtc tggcgagatc atgctttgaa ttgtcggttg tggagtatcg tcatcatcat
     3481 catcatcatc gtcgtcgtcg tcatcgtcgt cgtcatcgtc atcatcgtcg tcatacccta
     3541 caggtttggg aggggtctca ggctgtggtt gtggcacttc ctcccttatc ctatcaaaat
     3601 cattggagtc gattatcctc tcaaggtcaa gattcattcc ctgacttagt ctgaccaggt
     3661 cctcaatgct taggccagcc aggtcctcaa cttgaagctc tgggtttagg attataaggt
     3721 tcctgataaa ggtcatcttg ctgacagaaa tactgtattc ccttgcatag ctctctatgg
     3781 tagatgtgtt ttcaagtttt tgagaaagta caacaggctg aaggtctttt gcctgcagat
     3841 actggtgaat ccttgtgtca agttccctct tctgcctttc tgccttgtcc tggtcctggt
     3901 tgtaaacaga caggagtagg aactcgtggt ccttgtcaag gtatctttca tccatcatcc
     3961 tggtcaggat ctcttctgtg acctcatata ctgtcatccc cttgtagtcc aagtccctgg
     4021 tcaagccttg tccatcacca ttttctgcca ccaagccgat aaccttatcc tgcctgttgg
     4081 tcgtcatctg gatgctggga tttacgtcca ggtatattct gctgtccggc atccttgttc
     4141 ctagctgcca gccaaggaat accaaaacaa atgcagctgc cagggatgcg tagggcaggt
     4201 atcttctata attatatggt cctgattgct gcttggttat ttcatcgtgc ctaagcatcc
     4261 ttgtccttgg agtacttttt atattgtcca ggatatcaat tggtgcctga ttcatagatc
     4321 ttttgacact atcaagtatt tgcttgtcct tcatgccctc gcctcctttc cttccaggta
     4381 ttcccttaat tttttcagtg ccctgtggta ttttgagagg gttgtggaca ggttaagtcc
     4441 cagcccctcg gctatttcct tgtgcttcat tcctgatact gcatggagca ttacaatctc
     4501 cctttcctcc tgggtaagaa gtgtcaggac cccctccaac actatccggt catccatatc
     4561 agttacatag gaaaatctcc tgtcatctgc cacctcttca ggctccatgg atatgctcct
     4621 cttttccttt tttgtattgg aatagtgaag gttcttggct atggtgaaca tccaggccag
     4681 gggctttccc attggcttgt agagatgtgc agcggaaagg atctttacat aggtatcctg
     4741 cataaggtcc agcgccatct ggtggtccct tgtaagggag accgagtatg catacattgt
     4801 cctttccgta agggcataca ggtcatcaag ggcagacatg tcgtccttgg caagccttct
     4861 aaaaagtcct tcatctattt ctattctctt gtggcccttg tcattcggat tgtcatcata
     4921 gacaattgta aataaaatca tcttcatggc tcctttgata gtataacttc tgaatggtgg
     4981 attttattgc attaaaaata ttattatcta tacccaaaat gaaaaaaaca caacgggacc
     5041 ggttcttttt gtgttttttg gttaaaaaac aaggcccgcc atcaagacgg gaccttgctt
     5101 tatattgggt gtggggtggc acaccttttg ggttttaccg atataaatca gttgtttttg
     5161 gttttatcct tgttttgtcc tggtgcttaa tttgattttc cagggcttgc tggtcttccc
     5221 aattcaccat tttcttcatc ttcatcatct attgcatctt cgtcattatc atttgcttca
     5281 agtttcttta cctgtcctgg agcaaattgt gaaggattgt ccctttcgtc tttttcatcc
     5341 ttgtccgcct gtcctggtgc atttacacta ggattgtttt tctccccatt tctggctgaa
     5401 aactcttcca tgattctctt tgacagttcc ttgttgtcgg tatcctcgtc ctcatctatg
     5461 ccgtactcac ttctgatgcg atttaggtac ctgtcaacat tttctacttc cataaggtta
     5521 gtgatgatat tgtatttgcc cggtgtgatt ccaagttctc tagctctttc aacatcttca
     5581 ggactcaagc cttttactga catattttct tctctagctc ttaatccttc gttgtcgtaa
     5641 tcatcgtcgc ccttttcctc ttgagctctt tccctggctt cttcagcttt ttcacgagca
     5701 tcttcagctt ttttctcagc ttcttctctt ctctcttcaa tcattgccct gatctcttct
     5761 gcaagtttct ctgcatcttc ctcgctgtct cccatgggag ctatagcttc tatgtcatac
     5821 ttgtcaaaaa gctcctcaag aaaatcttta atgtcactat tctcgaaact gatttcctct
     5881 ccatctcgtt caacacttgt aactttccct ctcctgttta ccttaatcat gaactcagtt
     5941 ccgtcagttt ccaaagcatt caattcataa tcatccgcat atacccaggt gcctgtacca
     6001 atcatagcaa atatcaatag t
//
LOCUS       DASRQF010000113        10360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1781464, whole genome shotgun sequence.
ACCESSION   DASRQF010000113 DASRQF010000000
VERSION     DASRQF010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 10360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10360
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1781464"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VFD79_02900"
     CDS             <1..339
                     /locus_tag="VFD79_02900"
                     /inference="COORDINATES: protein motif:HMM:NF025582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4330 family protein"
                     /protein_id="HZH93027.1"
                     /translation="VMTVEVDELKPEMVTDLKAGDQIFSQYRLQNATIREVETRPMEI
                     KAVNGQGEVQVIESQDLVTLQAVIDAQVVTSGPYMDLGGQEVKVGLTFVLKTTEFEAM
                     SDIKHIEVAQ"
     gene            336..830
                     /locus_tag="VFD79_02905"
     CDS             336..830
                     /locus_tag="VFD79_02905"
                     /inference="COORDINATES: protein motif:HMM:NF025582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4330 domain-containing protein"
                     /protein_id="HZH93028.1"
                     /translation="MKMMNKEGKVFNRINILDLIFIAAIILLLFLSAIKIMGKDIEDI
                     GSSAEYVQVRFVASIPYERGYLDSIKVGDTIGETKQYLQLEVVGVEVHPVYETNLDME
                     GNPVRSLDPTMEEARVTIEGTLPYESMSYKLGAQEVRQGKIIFIESDFYRLKGQIETI
                     EVVN"
     gene            880..2394
                     /locus_tag="VFD79_02910"
     CDS             880..2394
                     /locus_tag="VFD79_02910"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZH93029.1"
                     /translation="MKIKYFAENSFIFSISLNKDKDSAGKEYRSWIQTSLSYSLLMQM
                     DKLARTVEGHIYRGIRGSKFLGWTFKEDKGQTGSAEAGGILGNSLIYKGFNRVLNSPK
                     WTDLLMMLPVLYVAVDYVIRRLPVINRFGSVWDELLLLLMAGVFILKRIASGGRIKYN
                     FGPMDLPVIIYIIMGISHVLIKAPDLGIAIEGFRSVFQHILWYFIATQYIRTVRDSER
                     IIKVMMALGLFLGLHAVYQYIVKVPMPGNWVDTAENITTRAFSIIGSPNILGVIFVLF
                     IPIGVAMTLSAKKPIGRLYYGGVSLVMLAGLLFTMSRGAWLAFAFAAGIFVIILVPKL
                     IIPFAGLGGAFVLFGGALSDRLLYMLSPVYLMKSAAGGRLYRWNIGMEIWRRDTSFGM
                     GLGRYGGAVAMNNNLTPFYLDNYYLKTLTEMGIYGMAALVFVIISFIVSTAKIIRSQT
                     VLRYKVMVIGLFAGAMGVLAQNFVENIFEVPAMIAYFWITVALINTYSPTGEDE"
     gene            2413..4617
                     /gene="csaB"
                     /locus_tag="VFD79_02915"
     CDS             2413..4617
                     /gene="csaB"
                     /locus_tag="VFD79_02915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HZH93030.1"
                     /translation="MRVLHLISGGDTGGAKTHIIALLKGLNKEMDAQIICFIEDTFYD
                     DVVKAGIPIKVFKQKSRSDLTIIGRLVHEIQEKGYDLVHCHGARANFIAHFLKGKVNV
                     PFVTTIHSDYKLDFKDNFYKRLVYTTLNTIALRRFDYYIGVSDLFRKMLVDRGFRRDR
                     IFVTYNGIDCDSEESFVPREEFLARYGIEDRGQTFIGIAARLDKVKDHVTFIEAAKLV
                     LQEEDATFLIAGEGGEAEYLKELVRRHGIADNVKFLGFVQDPYSFFNAIDINTLTSVS
                     ESFPYVILEGAKMMKPVVTTQVGGVHMLVENGENGYLVPVGNEVILRDRFLKLIRDRD
                     LREEMGRKLFMAVRKKFSYAAMAQSHISIYEKILHGGPNVVMSGYFGFDNSGDDAILK
                     AIVKDMKEHNRLVRINVLSKDPDKTESICPVVSSDRFRIRDVYKSLRDSDILISGGGS
                     LLQDVTSTRSLLYYLALMRLAFLLRKPVMVYANGIGPVNRPFNRYLTKNTLNKVQQIT
                     LRDRGSLEYIQKLGVTNKNVKVTADPVFTLEAVDNNRVNQIFINENIYPNKPLVGVSV
                     RYWKDDKNLSREISKALQEIIDEKNIEILMIPMHYPEDLEICQGIREMVNREECHILQ
                     AKYTAEEIMGIIGKLEMMVAMRLHSLIYAATRKVPMMGLIYDPKVEGLLRELEIPYSV
                     SVDNLEAASFKEVFHAAWDDLDKMRENISEKEERLKALAQENIELAFRITGR"
     gene            4622..5407
                     /locus_tag="VFD79_02920"
     CDS             4622..5407
                     /locus_tag="VFD79_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HZH93031.1"
                     /translation="MDRTDKVDIFGVEIQNTTLKDLMGELEKSLKGGGLAKVFTPNTE
                     IVMAAKKSESLKGELNKGTYVIPDGIGLIYGAKIRGITLKERVTGFDTSMELLRIADQ
                     NGYGLYLLGGKEGIAEEAKEQIQKDYPGIRVTGCHHGYFKGSHTGDGPTPEEERIIDE
                     IKTSRTDILFVGFGFPRQEIWINTYGEDLGVKVAIGNGGVIDILAGQAKRAPDIFIRL
                     GLEWFYRLVTNPSRIKRQAVLPLFLVEVALNGSSIHKISKEDK"
     gene            5407..7380
                     /gene="tkt"
                     /locus_tag="VFD79_02925"
     CDS             5407..7380
                     /gene="tkt"
                     /locus_tag="VFD79_02925"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HZH93032.1"
                     /translation="MNIDQLSIDTIRMLSAEGVEKANSGHPGLPLGAAPMAYTLWSKV
                     MNHNGKNPDWTNRDRFILSAGHGSMLLYSLLHLFQYGLEMEDIKDFRQLDSKTPGHPE
                     YGHTAGVESTTGPLGQGISTAVGMAMAEAHLAANFNTEDMKIIDHYTYVLVGDGDLME
                     GISNEASSLAGTLGLGKLIVLYDSNSISIEGSTDLAFTENVRGRYEALGWDTILVQDG
                     TDIDAIENAIRKAKENLHKPTLIEVITNIGYGTEKQDSASAHGEPLGDHNMEVLKKNL
                     KWDHDPFTVPDEVKSHMEGLVDEGQEKEDKWNQLLEDYGKAYPDKARELQEWLSGKVP
                     EDYLESDEFWSFDKAVATRAASGTVLNRLAEKMPNLFGGSADLAPSNKTNMTSREVFS
                     KDNYAGSNIHFGVREHGMGAALNGMALHGGVKPYGGTFFIFTDYMKPSMRLASLMELP
                     VTYVLTHDSIGVGEDGPTHQPVEQLAVFRAQPNFIDFRPADARETAAGWYVAVKSEKT
                     PVGLILTRQGLPLLEGTGKEAIKGGYVLKKEKKDLQLILMASGSETSLIYEAADKLED
                     QGIGTRVVSMPSWELFEQQTREYRDEVLPSSVRARLAVEAGSSLGWSRYTGLDGDVIS
                     LDRFGGSAPANQLFEKFGFTVENVVEKAKALLK"
     gene            complement(7423..8016)
                     /locus_tag="VFD79_02930"
     CDS             complement(7423..8016)
                     /locus_tag="VFD79_02930"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HZH93033.1"
                     /translation="MDKKTIREQILEERSQLSESAHKSHSRKIIDLLKATKYYKEAKV
                     VMCFVSFNDELDTHQFIKEALAEGKRLIIPITVQATKELIPSELKDFSELEPGYYNIL
                     TPKKEFIRPVEQSDIDLVVVPGVVFDPFGYRVGYGGGYYDRFLSSIARTVPKIAVGFG
                     MQLVDRVPRESFDIPVDMIITEKGVTPCSEGLYETRP"
     gene            8133..8828
                     /gene="ftsE"
                     /locus_tag="VFD79_02935"
     CDS             8133..8828
                     /gene="ftsE"
                     /locus_tag="VFD79_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HZH93034.1"
                     /translation="MIKMVNISKEYRNGVTALYDINMEIDKGEFVFLVGSSGAGKSTL
                     IKLLLKEEGISRGRMYLDGMDITRVPNRRIPYIRRKVGVVFQDFRLLPNKTVYENVAF
                     AMEIIGAKPREIRRRVPLILSMVDLSRKASSFPDQLSGGEQQRVSIARSIVNNPPVLI
                     ADEPTGNLDPDTAEEIMKVLVDINNRGTTIVMATHARDIVNKMKKRVIALEDGRLIRD
                     EEKGGYSIEIPNI"
     gene            8809..9705
                     /gene="ftsX"
                     /locus_tag="VFD79_02940"
     CDS             8809..9705
                     /gene="ftsX"
                     /locus_tag="VFD79_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HZH93035.1"
                     /translation="MKFRIFKNIIKQGFQSMWRNRGMGIASITSISAVLMILGVVLIL
                     VLSINNAVLDTKLKFDEVEVFLVDDLPSQSMDRIEEAVKDHPGVLSIIFRSKQQALEI
                     MKEEWADEAYLLEGLETNPLPDSYIIKVNDIEYADGLVNDVKTLDGVEEVKYYKDVID
                     KLLIFAGYIQFGGILMIGILVFISVFIISNTIKITVSSRRREINIMKYVGATNGYIRG
                     PFIMEGIFLGIIGALISIFIVYFGYQYFYKSMNEKLYDLFTFYLVDPRLIFADLAIMF
                     GTIGAGIGALGSLVSLKRFLNV"
     gene            9717..>10360
                     /locus_tag="VFD79_02945"
     CDS             9717..>10360
                     /locus_tag="VFD79_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HZH93036.1"
                     /translation="MRGRKLLILFIAFLMVLGTVMQASGQSVDDLRKQQEQIQNQIEE
                     TKKEIQNMQNQTKDIDNQIKELDLKVSSAALELESVEKEIEAINRNIEITEEELEIAK
                     NNIDDKIDTFNQRLRVMYKNGNTGYLEVLLSAGNIKDFLSRQEMIQSIAEYDKDLIRY
                     MKEQRDIIEEKKVQLEGQRASLEVTRSKVESRKRDLEQASRAKEQLMGKLEQDI"
BASE COUNT         3156 a         1984 c         2648 g         2572 t
ORIGIN      
        1 gtcatgacag tggaggttga cgagctgaag ccggaaatgg ttacagacct taaggcagga
       61 gaccagattt tttcccaata tagactacag aatgcaacca taagagaggt agagacaaga
      121 cctatggaga taaaggcagt gaacggtcag ggagaggttc aggtgataga aagccaagac
      181 ctggttacac tccaggcggt tatagatgcc caggttgtaa caagtggacc ctacatggac
      241 cttggtggcc aggaagtcaa ggtaggtctc acctttgtgc tcaagaccac agaatttgag
      301 gcaatgagtg atataaagca tatcgaggtg gcacaatgaa gatgatgaac aaagagggta
      361 aggtatttaa tagaataaac attttggacc tgatatttat tgcagcaatt atactgctct
      421 tgtttttgtc ggcaatcaag ataatgggca aggatatcga ggatataggg tcatcagctg
      481 aatacgtaca ggtaaggttt gttgcttcaa taccctatga gaggggatat ctggactcaa
      541 tcaaggttgg ggacacaatt ggagagacaa agcaatatct ccagctagag gtagtgggag
      601 ttgaggttca tccagtctat gaaacaaatc tcgacatgga aggaaaccca gtaagatctc
      661 ttgatcccac catggaggag gcaagggtga ccatagaggg aacccttccc tatgaaagca
      721 tgagctacaa gcttggagcc caggaagtca gacagggcaa gattattttc attgaaagtg
      781 acttctatag acttaaggga caaatagaga ccattgaggt tgtgaattag tatgggaatc
      841 aaaagtgctt tgaaaaacag cttttttgta ggcttgctta tgaaaattaa atattttgca
      901 gaaaacagct ttatattcag cataagcctc aacaaggata aggattctgc cgggaaagag
      961 tacaggtcct ggatacaaac cagcctttcc tacagcctgc tcatgcagat ggacaagctt
     1021 gcgcggacag ttgagggaca catttatagg ggtattcggg gaagcaagtt ccttggatgg
     1081 acatttaagg aggacaaggg ccagaccggg tctgcagagg caggagggat acttggaaat
     1141 agcctgatct acaaaggatt caacagggtc cttaactcgc ccaagtggac agacctgctt
     1201 atgatgttac ccgtcctgta tgttgccgtg gactatgtaa tcagaaggct tcctgtcatt
     1261 aacaggtttg gttccgtatg ggacgagctt ctactcctat tgatggccgg agtatttata
     1321 ctcaaacgaa tagccagcgg aggaaggata aagtataatt tcggtccaat ggaccttcct
     1381 gtgataatct atattatcat gggaatcagt catgtcctga tcaaggcgcc agaccttggt
     1441 atagccattg agggcttcag gtctgtattt cagcatatac tatggtattt cattgcaaca
     1501 caatatataa ggacagtcag ggattcggaa aggatcataa aggttatgat ggctcttggg
     1561 ctcttcctgg gccttcacgc agtctatcag tatattgtca aggtaccaat gcctggaaac
     1621 tgggttgata cggctgagaa cataacaacc agagctttct cgatcatagg tagcccaaat
     1681 attctagggg taatatttgt ccttttcata ccaattggag tagccatgac cctgtctgcc
     1741 aagaaaccca ttgggaggct ttattacgga ggcgtttccc tagtaatgct cgcaggctta
     1801 cttttcacca tgtccagagg cgcatggctt gcatttgcct tcgctgccgg aatatttgtc
     1861 attatactgg ttcccaagct tataatacca tttgcaggtc ttggaggagc atttgtactt
     1921 tttggaggtg ccctttcaga cagactcctc tatatgctgt cacccgtgta cctgatgaaa
     1981 tcagccgccg gtggtagact ttacaggtgg aatatcggga tggagatatg gagaagggat
     2041 acaagttttg gaatgggtct ggggagatac ggtggtgcag ttgccatgaa caacaacctg
     2101 acaccattct acctggacaa ctactatttg aagaccttga ccgagatggg gatatatgga
     2161 atggctgcac ttgtttttgt gatcatttca tttattgtat caactgccaa gatcattagg
     2221 agtcagacgg tcttaaggta caaggtaatg gtgatcggac tctttgcagg tgcaatggga
     2281 gtgcttgcac agaactttgt tgaaaatatt tttgaagtgc cggccatgat tgcatatttt
     2341 tggataacgg ttgcacttat aaacacctac agtcctactg gagaggatga ataggatgaa
     2401 aggagaatac cattgagggt acttcactta ataagcggcg gtgacacagg tggcgcaaag
     2461 acccatataa tagccttgct aaagggattg aacaaggaaa tggatgcaca gatcatatgc
     2521 tttatcgagg acaccttcta tgatgatgtg gttaaggctg ggatacctat caaggtattc
     2581 aagcaaaaga gcaggtctga ccttaccata ataggcagat tggtccatga aattcaggaa
     2641 aagggctatg acctggtcca ttgtcacggg gcaagggcga attttattgc acattttctt
     2701 aagggcaagg taaatgtccc atttgtaaca acaatccaca gcgactacaa gcttgatttc
     2761 aaggacaact tctacaagag gcttgtatat accaccttga acacaattgc attgagacgg
     2821 tttgattact atattggagt atcggacctg ttccggaaga tgctggtgga cagaggcttc
     2881 agacgtgaca ggattttcgt gacctataac ggaattgact gcgattcaga ggagagcttt
     2941 gtacccaggg aggaatttct tgcaagatac ggcattgagg ataggggtca gaccttcatt
     3001 ggaattgcag caaggcttga caaggtgaaa gaccacgtga cctttataga ggctgccaag
     3061 cttgttctcc aggaggagga tgcaaccttc ctgattgccg gtgaaggtgg ggaggccgag
     3121 taccttaagg agctggtaag aaggcatgga atagctgata atgtaaaatt ccttggcttt
     3181 gtccaggatc cttattcctt cttcaatgca attgacatca acacacttac ctccgtcagt
     3241 gaaagctttc cctatgttat actggaaggt gcaaaaatga tgaaacccgt tgtcaccact
     3301 caggttgggg gtgtccatat gcttgttgag aacggtgaga acggttacct ggttcccgtt
     3361 ggcaacgagg ttatcctaag ggaccgattc cttaaactca taagggatag ggacctgagg
     3421 gaggagatgg gcagaaagct ttttatggca gtcaggaaga aattctccta tgctgcaatg
     3481 gcccaatcac atataagcat ctatgagaaa attcttcacg gagggcccaa tgttgttatg
     3541 agcggttact ttggatttga taacagtggg gatgatgcca tactgaaggc aatagtaaag
     3601 gatatgaagg aacacaaccg gttggtgaga ataaatgttc tttcaaagga tccggacaag
     3661 acagagagca tctgtccggt tgtatcctcg gacaggttca ggataaggga tgtctacaag
     3721 tccttgaggg actcggatat cctaattagc ggtggcggga gcctgcttca ggatgtaaca
     3781 tcaactaggt cccttctgta ttatctggcc cttatgagac tggcatttct tttaagaaag
     3841 cccgtaatgg tctatgccaa tggaattgga cctgttaaca ggccctttaa ccgttatctg
     3901 acaaagaata ccttgaacaa ggttcagcag attaccctaa gggacagggg ctcccttgaa
     3961 tacatccaaa agctgggtgt gacaaataaa aatgtaaagg tcacagcaga cccggtattc
     4021 acactggaag cggtggacaa caatagggtt aaccagatat ttatcaatga gaacatctat
     4081 ccaaacaagc cccttgtggg ggtttcagtg agatattgga aggatgacaa aaacctgtcc
     4141 agagaaataa gcaaggccct acaggaaatc atagatgaaa agaatattga aatcctaatg
     4201 attccaatgc attatcctga ggacctggaa atttgccagg gaatcaggga gatggtaaac
     4261 agagaggaat gtcatatact ccaggccaaa tacacagcgg aggaaatcat gggcataatc
     4321 ggcaaactgg agatgatggt ggcaatgagg ctacactcac ttatttatgc agccaccagg
     4381 aaggttccaa tgatgggcct gatatatgat cccaaggttg aaggactact tagggagctg
     4441 gagattcctt attcagtgtc cgtagacaat ctggaagcag ccagtttcaa ggaggtattc
     4501 catgcagcct gggatgatct ggacaagatg agggaaaaca taagtgaaaa agaggaaaga
     4561 ctcaaggccc tggcacagga aaacattgaa ctggctttcc gaataacagg caggtgattg
     4621 gatggataga actgataagg tagatatatt tggggtagaa atacaaaata caacattaaa
     4681 agacctgatg ggtgaactgg agaagtccct taaaggtggc ggtttagcaa aggtattcac
     4741 gccaaacact gaaatagtca tggcagcgaa aaagtctgaa agcctgaaag gggagctcaa
     4801 caagggtacc tatgttatac cggatggaat agggctcatt tatggtgcta aaataagagg
     4861 aatcacccta aaggaaaggg tgacaggttt tgacacatcc atggaactcc tccgcattgc
     4921 ggaccagaat ggatacggcc tataccttct cggaggcaag gaaggaattg ctgaagaggc
     4981 caaagagcag atccagaagg attacccggg aataagggta acaggctgtc atcacggtta
     5041 ttttaaggga agtcacaccg gtgatggtcc aactcctgag gaagagagga tcatagatga
     5101 aataaagacc agccgtacag atatcctttt tgttggtttt ggttttccca gacaggaaat
     5161 ctggatcaat acctacgggg aagaccttgg agtaaaggtg gccattggaa acggaggagt
     5221 tatagacatc ctggcaggcc aggccaaaag ggctccggat attttcataa ggctgggcct
     5281 tgaatggttt tatcgtcttg tgactaatcc ttcaagaata aagagacagg cggttcttcc
     5341 actatttctt gtggaagtgg cccttaatgg aagttcgatt cataaaataa gtaaggagga
     5401 caagtaatga atattgatca gttaagtatt gataccataa gaatgctttc agcggagggt
     5461 gttgaaaagg caaattcagg tcatcctgga cttcctttgg gagcagcccc catggcatat
     5521 acactatgga gcaaggtaat gaaccataat ggaaagaatc cagattggac aaacagggat
     5581 aggtttatcc tatcggctgg ccatgggtca atgctccttt actcacttct acaccttttc
     5641 cagtacggac tggagatgga agacatcaag gactttaggc agctggacag caagacgcct
     5701 ggacaccctg agtatggaca cacagcagga gttgagtcca caacaggacc tctaggacag
     5761 ggaatctcca cagcagttgg gatggccatg gccgaggccc atcttgcagc caacttcaac
     5821 accgaggata tgaagataat cgaccactac acatatgttc ttgtgggaga tggcgacctg
     5881 atggagggta taagcaacga agcttcatcc cttgcaggca ccctgggact tggcaagcta
     5941 atagtgcttt acgattcaaa ttccatttca attgagggta gcaccgacct tgcattcact
     6001 gaaaatgtaa ggggaagata cgaggccctg ggatgggaca ccattctagt tcaggatgga
     6061 actgatatag acgcaattga aaatgcaatc aggaaggcca aggaaaatct tcacaagcca
     6121 accctgatcg aggttattac aaacataggc tacggaacag aaaagcagga tagtgcaagt
     6181 gcccacgggg agcctcttgg tgaccacaac atggaggtcc tgaaaaaaaa cctgaagtgg
     6241 gaccatgacc cattcactgt accagatgag gtaaaatccc acatggaagg cctggtggat
     6301 gaaggccagg aaaaggaaga caagtggaac cagctacttg aggattatgg aaaagcatat
     6361 ccagacaagg ccagagaact tcaggaatgg ctttcgggaa aagtgcctga agactatctt
     6421 gagtcagatg agttctggtc ctttgacaag gcagttgcaa caagagctgc gtctggaacg
     6481 gtccttaaca ggcttgcaga aaagatgccc aacctctttg gcggatcggc agacctggca
     6541 ccatcaaaca agacaaatat gacctcaagg gaagtgttct caaaggataa ttacgcagga
     6601 tccaacatcc actttggggt tagggagcat ggcatgggtg cagcacttaa tggaatggcc
     6661 cttcatggag gcgttaagcc atatggtgga accttcttca tattcaccga ttacatgaag
     6721 ccatcaatga ggcttgcatc acttatggag ctaccggtga cctatgttct cacccatgac
     6781 agcatagggg ttggagagga tggacccacc caccagccag tggaacagct ggcagtgttc
     6841 agggctcaac ctaatttcat tgacttcagg ccagcagatg caagagaaac agctgccggt
     6901 tggtatgttg cagttaaaag cgagaagact ccggttggtc tgatcctaac aagacagggg
     6961 ctcccccttc ttgaaggaac aggaaaggaa gcaatcaagg gaggatacgt ccttaagaag
     7021 gagaaaaagg accttcagct tattcttatg gcttccggct ctgaaaccag cctgatttat
     7081 gaagcagctg acaagcttga agaccagggg ataggcacca gggttgtaag catgccttcc
     7141 tgggaactgt ttgaacaaca gacgagagaa tacagggatg aggtattgcc atcttcagtt
     7201 cgggccagac tggcagttga ggcaggcagc tcattgggat ggtccagata cacaggactt
     7261 gacggggatg ttatttccct ggacaggttt ggtggttcgg ctcctgcaaa ccagcttttt
     7321 gagaaatttg ggtttacagt tgagaatgtt gtggaaaagg caaaggccct cctaaaatag
     7381 caaaaaacaa tgaagcagct agaaaaattc tagctgcttt ttttagggtc ttgtttcata
     7441 caatccttct gagcagggag ttacgccctt ttcagtaatt atcatgtcaa caggtatgtc
     7501 gaagctttct cttggaaccc tgtccacaag ctgcattcca aatccaactg caatcttggg
     7561 aacggtcctt gcaattgagc tgaggaatct gtcataataa ccgccaccat agcctaccct
     7621 gtatccaaat ggatcaaaca caactccggg aaccacgacc aggtcgatat cggattgctc
     7681 aacaggtcta atgaactcct tcttgggagt gagtatgttg taatagcctg gctcaagctc
     7741 actgaagtcc tttagctctg aaggaataag ctccttggta gcctggacag tgataggtat
     7801 aatcaacctc tttccctctg ccagggcttc cttgataaac tggtgagtgt ccagctcatc
     7861 gttgaagctg acaaagcaca ttaccacctt ggcttccttg tagtacttgg ttgccttaag
     7921 taaatctatg atttttctac tatgggattt gtgcgctgat tcacttaact gggatctttc
     7981 ctctaaaatc tgttctctga tagttttttt gtccaattaa atcacctcaa acagtttatt
     8041 tatatgctat cttgtaatat aataactatg ataccatgaa aaagagatat gtcaaaacta
     8101 aaattgcatg ggaaggattt tgaggtgaga aagtgataaa aatggttaat atttccaagg
     8161 agtaccgcaa cggcgttact gccttgtatg atataaatat ggagatagac aagggagaat
     8221 ttgttttttt ggtaggctca agtggagctg gcaaatcaac acttattaag cttttgctta
     8281 aggaagaggg tataagccgg ggaaggatgt acctggatgg gatggacatt acaagagtcc
     8341 ccaacaggag gatcccttat atcaggagaa aggttggagt tgtctttcag gacttcagac
     8401 tcctgcccaa caagaccgtg tatgaaaatg ttgcgtttgc aatggaaata ataggagcta
     8461 agccaaggga aatcaggaga agggttcccc ttattttgag tatggtggat ttgagcagga
     8521 aggcatccag ctttccagac cagctatcag gtggggagca gcagagggtc tcaattgcaa
     8581 ggtcgatagt caacaaccct cctgtactaa tagccgatga gccaacggga aatcttgacc
     8641 cggatacggc agaagaaatt atgaaggttc ttgtggacat taacaataga ggaactacaa
     8701 tagtcatggc aacacatgcc agggatatcg taaacaaaat gaaaaaaagg gtaattgccc
     8761 ttgaagacgg gagattgata agggacgaag agaagggtgg ttatagcatt gaaattccga
     8821 atatttaaga atatcatcaa gcagggattt cagagcatgt ggcgtaaccg cggcatggga
     8881 attgcatcca taacgagcat atcagccgta cttatgattt tgggtgttgt cctgatcctg
     8941 gttctaagca taaacaatgc agtactggac actaagctca agtttgatga ggtggaggta
     9001 tttctggttg atgatcttcc aagtcagtcc atggatagaa ttgaggaagc cgttaaagac
     9061 catcctggtg tcctttcaat catattcagg tcaaagcagc aggcccttga gatcatgaag
     9121 gaggaatggg ccgatgaggc ttacctcttg gagggtttgg aaaccaaccc cctgccggat
     9181 tcatatataa tcaaggtaaa tgatatagaa tacgctgatg gactggtaaa tgacgtaaaa
     9241 acacttgacg gcgtggaaga agtgaaatat tataaagatg taatagataa attgttgatt
     9301 tttgcaggat acatccagtt tggaggtata ttgatgatag gaatacttgt attcatatcc
     9361 gtattcatta tctccaacac cattaagata accgtatcct caaggagaag agaaataaat
     9421 atcatgaagt atgtaggagc cacaaatggc tatataagag ggcccttcat tatggaggga
     9481 atatttctgg ggataattgg agcccttata tccatattca ttgtgtattt tggctaccag
     9541 tacttctaca aatcaatgaa tgaaaaactg tacgaccttt tcacattcta tttggttgat
     9601 ccaaggttga tttttgcgga tcttgcaatt atgttcggta caattggagc cggaattggt
     9661 gcactgggta gtctcgtgtc attgaagaga ttccttaacg tatagggggt aaatatatga
     9721 gaggaagaaa attactgatt ctattcatag catttctcat ggtgttaggg actgtaatgc
     9781 aggcaagcgg acagtctgtt gatgatttga ggaagcagca ggagcagatt cagaaccaga
     9841 ttgaagagac caagaaagag attcagaaca tgcagaacca gaccaaggat atagacaacc
     9901 agatcaagga attggatctg aaggtttcca gtgcggcact tgagctggaa agcgtggaga
     9961 aggaaattga agcaatcaat agaaacattg aaattaccga ggaagaactg gaaatagcca
    10021 agaacaacat agatgataag atagacacat tcaatcaacg actgagggta atgtataaga
    10081 atggtaatac cggttatctt gaggtccttc tttcagccgg aaacataaag gacttccttt
    10141 caaggcagga aatgatccag tccattgcag aatacgacaa ggaccttatc aggtacatga
    10201 aggagcaaag ggatatcata gaggaaaaga aggtccagct tgaaggtcaa agggcatccc
    10261 ttgaggttac aagatccaag gttgaatcca gaaaaaggga cctggagcag gccagcaggg
    10321 ccaaagagca gcttatgggc aaacttgagc aggacataaa
//
LOCUS       DASRQF010000114         7845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_884932, whole genome shotgun sequence.
ACCESSION   DASRQF010000114 DASRQF010000000
VERSION     DASRQF010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 7845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7845
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884932"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..860
                     /locus_tag="VFD79_02950"
     CDS             42..860
                     /locus_tag="VFD79_02950"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HZH93037.1"
                     /translation="MEIKLIAIDMDGTLLDSNNGLSERTRRAIEMAKEKDVEVVLATG
                     RVLKSALHYAEMLNLDSYIAACNGAIIVDRQGKDVYRKPIDKKKLEKIMEIGHNMSVY
                     FHFYNEDTFFTRTYVKEIVEYYSSSKGKFTGQSIDVDIYESTEDILNRRDLDIFKFLF
                     IDNNSDKLQQLRDSLHSVDGISVSKSWANNLEVMEEGASKNKSLDHLCKLMNISLENT
                     MAIGDNENDLSMLQIAGLSVGMGNGARSVLDQVDFVTEDNDNDGVAKAIERFVL"
     gene            883..1350
                     /locus_tag="VFD79_02955"
     CDS             883..1350
                     /locus_tag="VFD79_02955"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HZH93038.1"
                     /translation="MALNIVLVEPEIPHNTGSIARTCALTGTVLHLVRPLGFSITDKH
                     LKRAGLDYWPLVKLKYYDSFQEVLDNNPDGDFYFASTKAKKNYTQFNYRDNSFFVFGR
                     ETKGLPESLVMEHLDTAIRIPMKKNIARSLNLSNSANIIIYEALRQLGFPDLE"
     gene            1420..3027
                     /locus_tag="VFD79_02960"
     CDS             1420..3027
                     /locus_tag="VFD79_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HZH93039.1"
                     /translation="MDTALPVLGGLGLFLFGMNTMGDGLEKAAGNKLKKLIERLTKNR
                     LMAVLVGIVVTIAVQSSSATTVMVIGFVNAGLMTLPQAVGIIMGANLGTTVTAQLVAF
                     KLTEVAPLAVAIGVAIWIGAKNKKAKTYSEILIGFGILFVGMDMMGDGLKPLADLPAF
                     ANVMTSLNNPFLGMLVGLGLTTIVQSSSASIGLLQALAGEGLIQMNIAFPILFGENIG
                     TTTTAMISSIGANKTAKRAAIIHFLFNLAGTIMFMTIFRYPIEWLVTYISPNDVQRQI
                     ANAHTSFNLINLVVQFPFALLLVKAAEKLVPGEDIEEITESKFLDKRIIETPSIALGQ
                     ASREALRMGDLVLENLTSSRTAMLEDKIELIDSILERERVINRIQREITDYLVRLSNA
                     SLSDEQHADVNAMLYIIGDIERIGDHIENISELAQYKSDNNLYFTPQAMEEMDEMFSL
                     CAEVVQSALTAYKDTDEALARQVLILEDEVDRLERQNRTNHIDRLNKMMCSTGAGIIF
                     LDGISNLERISDHSRNIAMYVLDKFKG"
     regulatory      3147..3248
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3425..4363
                     /gene="trxB"
                     /locus_tag="VFD79_02965"
     CDS             3425..4363
                     /gene="trxB"
                     /locus_tag="VFD79_02965"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HZH93040.1"
                     /translation="MIYDVVIIGAGPAGLSAGLYAARARMSTLIIEKEAAGGQIATTD
                     EVANYPGSVEDATGPSLVARMVEQAEGFGAKRISDTIESVDVAGKIKILKGEKEEYQA
                     RTVIVATGAVPKHIGCPGEKELTGRGVSYCATCDGAFFEDLEVFVVGGGDSAVEEAMY
                     LTKFARKVTIVHRRDQLRAAKSIQEKALKNEKIEYLWDSVITELKGDGLLESMVVKNV
                     KTGEESEIFADEDDGTFGVFVFVGYDPANKLFEGLVQMENGYIITDDNMATNIPGVFA
                     AGDNRVKSLRQVVTATADGAIAATQAEKYIENNFEE"
     gene            4374..4691
                     /locus_tag="VFD79_02970"
     CDS             4374..4691
                     /locus_tag="VFD79_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002835444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZH93041.1"
                     /translation="MIELNKENYEEEVLTAEGTVLVDFWSPSCEPCKALMPHVHELEE
                     EFGDKIKFTSLDITKARRVAIGQKVLGLPVIAIYQNGERVDGVVGDEATTKSVKELVE
                     KYS"
     gene            4754..6040
                     /locus_tag="VFD79_02975"
     CDS             4754..6040
                     /locus_tag="VFD79_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HZH93042.1"
                     /translation="MRLELGKILIKDVVFGDSTKLDNGVLTINKSELIELVMEDEHIK
                     SVDVDIAKPGESVRITPVKDVVEPRVKVEGPGGVFPGVLSKVDVVGSGKTNVLKGCSV
                     MTTGKIVGFQEGIVDMCGPGADYTPFSKLINIVVNCEPVDGLKQHDHEKAVRFAGFKS
                     AAYIAETAKSVTPDEVEVFETDTFVDGMKKYPDLPKVGYVHMLQTQGLLHDTYVYGVD
                     AKQIVPTILYPTEIMDGAIVSGNCVSACDKNTTYHHLNNPVVHDLLAAHGKEINYVGN
                     IITNENVYLADKERSSNWTAKLAEYLNLDGVVISQEGFGNPDTDLIMNCKKIEEKDIK
                     TVIITDEYAGRDGASQSLADADKSADAVVTGGNANEVITLPKMDKVIGTLAYTDIIAG
                     GFDGSLADDGTITVEIQAITGATNEMGFNKMSAKGF"
     gene            6091..6564
                     /gene="grdA"
                     /locus_tag="VFD79_02980"
     CDS             6091..6564
                     /gene="grdA"
                     /locus_tag="VFD79_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_115322126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:6223..6225,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HZH93043.1"
                     /translation="MSIFNENSKVIVIGDRDGIPGPAMEECIKTTPAEVVFSATECFV
                     UTAAGAMDLENQKRVKDLAEKYGAENVVVLIGGGEAESAGLAAETVTAGDPTFAGPLA
                     GVPLGLKVYHAVEPEFKESVDADVYDDQIGMMEMVLDVDAIVEEVASMRKEHGKY"
     gene            6598..>7845
                     /gene="grdB"
                     /locus_tag="VFD79_02985"
     CDS             6598..>7845
                     /gene="grdB"
                     /locus_tag="VFD79_02985"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030700 - glycine reductase complex
                     [Evidence IEA];
                     GO_function: GO:0030699 - glycine reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:7645..7647,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HZH93044.1"
                     /translation="MAKIRIVHYINQFFAGIGGEEKADIKPEVREGAVGPGMAIQAGF
                     GGEAEIVATVICGDSYFGENIEAAKEEVLEMVKGYNPDLFIAGPAFNAGRYGVACGTI
                     TEAVQNELNIPALTGMYIENPGADMYKKAVYIVETKNSAVGMKDAVAKMVPLAKKLLK
                     GEEIGSPSEEGYIERGIRKNYFTEKRGSQRAVDMLLKKLKGEEFTTEYPMPNFDRVDP
                     NPAVKDLSKAKIALVTSGGTVPKGNPDHIESSSASKYGKYDIDGLNDLTEETHETAHG
                     GYDPVYANEDSDRVLPVDVLRDLEKEGRIGELHRYFYSTVGNGTSVGSSKKFAEEFSK
                     ELIADGVDAVILTSTUGTCTRCGATMVKEIERAGIPVVHICTVVPISLTVGANRIVPA
                     IAIPHPLGNPSMDKVEEKALRRKI"
BASE COUNT         2549 a         1367 c         1952 g         1977 t
ORIGIN      
        1 ctcaattggt atggtatatt atttgtgaca ggaggaatca tatggaaata aagctaatcg
       61 caattgacat ggatggcaca ctgctggata gcaacaacgg tttatctgaa aggaccagaa
      121 gagccattga aatggctaag gaaaaggatg ttgaagtggt tcttgccact ggtcgggttc
      181 taaaatctgc tctacattat gcagaaatgc tgaatctgga cagctatatc gcagcctgca
      241 acggagctat tattgttgac aggcaaggca aggatgtgta cagaaaacca atagataaaa
      301 aaaagcttga aaaaataatg gaaatcgggc ataatatgtc agtgtatttt catttttaca
      361 atgaagatac attttttact agaacctatg taaaagaaat tgtggaatat tattcatcat
      421 caaaaggaaa gttcacaggc cagtccattg acgttgatat atacgaaagc acagaggaca
      481 tattaaatag aagagacctt gatatattca agtttctctt tattgacaac aattctgata
      541 aactccagca actcagggac agtctccact ctgtggatgg aatttccgta tccaagtcct
      601 gggcgaacaa ccttgaggta atggaggagg gagcatccaa aaacaagagt ctggaccatc
      661 tttgcaaact gatgaatata tcccttgaaa acacaatggc cataggtgac aatgaaaacg
      721 acctttcgat gctccagatt gccggattaa gcgtagggat gggaaatggg gcaaggtccg
      781 tactggacca ggtagacttc gtcactgaag ataatgataa tgacggggtg gccaaggcaa
      841 tagaaaggtt cgtgctttga tcatattgac tggaggtata ggatggcttt aaacattgtt
      901 ttggtggagc cggaaatccc acacaacact ggcagcattg ccagaacctg tgctttgacg
      961 ggaacagtac tgcatcttgt aagaccattg ggattttcaa tcactgataa gcatcttaaa
     1021 agagcaggac ttgattattg gccattggtt aaattaaagt attatgacag ctttcaggag
     1081 gtgctggata acaacccgga tggggatttc tattttgcat ccaccaaggc taagaagaat
     1141 tatacacaat ttaattacag agacaattcc ttttttgtgt tcggtaggga gaccaagggt
     1201 ctaccggaaa gtctggtgat ggaacaccta gatacggcca tcaggatacc catgaagaag
     1261 aacattgcaa gatctcttaa tctatcaaac tcggcaaata tcataatata tgaagcatta
     1321 agacaattgg ggtttccaga tctggaataa taaattaaga accatgcaat aattactttt
     1381 gaattcttac aatcttagat aacaaagggg gaaactgaaa tggatacggc tcttccggta
     1441 ttaggcggct tgggattatt cctttttgga atgaacacca tgggcgatgg tttggaaaag
     1501 gctgcaggca acaagcttaa aaagctgata gaacgcctca caaagaatcg attgatggca
     1561 gtgctggtgg gtattgttgt aacaattgca gtacaaagca gtagtgcaac tacagtaatg
     1621 gtaattggtt ttgttaacgc agggcttatg accctgccac aggcagttgg aataataatg
     1681 ggtgcaaatc taggtaccac cgttacagca cagcttgttg catttaaatt gacagaggtc
     1741 gcacctttgg cagtggcaat aggtgttgcg atttggattg gcgccaagaa caaaaaggca
     1801 aagacatatt ctgaaatact tataggcttt ggtatactct ttgtcggtat ggacatgatg
     1861 ggggacggac tcaaaccatt ggctgatctt ccagcatttg caaatgtaat gactagcctt
     1921 aacaacccct ttcttggaat gcttgttggt ttaggtctaa caacaatagt ccagagcagc
     1981 tccgcttcaa taggactgct gcaggcactg gcaggtgaag ggttgattca gatgaacatt
     2041 gcattcccaa tcctttttgg ggagaacata ggaaccacaa caacagctat gatttcaagt
     2101 attggagcca acaagacggc aaaacgagcg gcaataattc actttttatt caacctggca
     2161 ggaacaataa tgtttatgac catattcagg tatcccattg aatggcttgt tacctatatt
     2221 tcgccaaacg atgtgcaaag gcagattgca aacgcacaca catccttcaa ccttataaac
     2281 cttgtcgtgc agttcccatt tgcccttctc ttggtcaagg ctgctgagaa gcttgtacct
     2341 ggtgaggata ttgaagagat cactgaatcc aagtttcttg ataagcgtat aattgaaacc
     2401 ccttcaatag ctcttggcca ggcatccaga gaagcattga ggatgggaga cctggtattg
     2461 gaaaatctca catcttcaag gacagccatg cttgaggaca agattgaatt aattgattca
     2521 atcctagaga gggagagggt tattaacagg attcaaaggg aaattaccga ttaccttgta
     2581 agactatcca atgcatccct ttcggatgaa cagcatgcag acgttaacgc catgctttac
     2641 attatcggtg atattgagcg gattggagac catatcgaga acataagtga gttggcccaa
     2701 tacaagtcag acaacaatct gtacttcaca ccccaggcca tggaggaaat ggacgagatg
     2761 ttcagtcttt gtgcagaggt tgtacaatct gcattgacgg cctacaagga tacagacgaa
     2821 gccctggcac gacaggtcct tatcctggag gatgaagtgg atagacttga aagacaaaat
     2881 cgaaccaacc atatagacag actaaacaag atgatgtgca gtacaggggc tggaattata
     2941 ttcctggatg gaattagtaa tcttgaaagg atatcggacc attcaaggaa tattgccatg
     3001 tatgtactgg ataaatttaa gggataatgt ttattaaaaa aaaggggaaa tacctattga
     3061 ttagtacaaa caaataaagt ataatgagct tgtcggagtt gttaaaaacg taacaaaata
     3121 ttttaaaaaa ttctgaacat ttctggatga ttgtagcggg agagaccact aagtggcgcc
     3181 gaaggagtaa ttaacagagc tagcccctct gttgcgaacc tctcaggcaa caggaccgtt
     3241 gccggacaga actctggaaa gcgttgttta caccgaagga gcaattttgt tctgtgaaga
     3301 atggaagaat ctctcaggtc aataaacaga gtgagtaggc atggtctatt tattctgttt
     3361 tttttttaga aaaatatgga ataatttgat accaactaat attaaccaag aaggagggaa
     3421 atggatgatt tatgatgtag ttattattgg agctggtcca gctgggcttt cagcaggctt
     3481 gtatgctgcc agagccagaa tgagcacctt gataatcgag aaggaagcgg caggtggaca
     3541 gatagccacc acggatgagg ttgctaacta tcctggatct gttgaagatg ccaccggacc
     3601 atcacttgta gccagaatgg ttgagcaggc tgaaggtttc ggtgcaaaaa gaatttctga
     3661 caccattgaa agcgtagatg tggctggaaa gataaagatt cttaagggtg aaaaggaaga
     3721 atatcaggcc aggactgtaa ttgtcgcaac aggagcagtg ccaaagcaca ttggatgtcc
     3781 aggagagaag gagctcacag gtagaggggt ttcctactgt gcaacctgtg acggagcttt
     3841 ctttgaggac cttgaagtat ttgtagtagg tggaggagac tcagctgttg aggaagccat
     3901 gtacctaact aagtttgcaa gaaaggttac cattgtccac cgaagagacc agttaagagc
     3961 cgccaagtcc attcaggaaa aggcattgaa gaacgaaaag attgaatatc tttgggattc
     4021 tgtaataact gagcttaagg gagatggact ccttgaatcc atggtagtca agaatgtcaa
     4081 gacaggtgag gaaagtgaaa tctttgctga tgaggatgac ggaacctttg gagtatttgt
     4141 ctttgtagga tacgatccgg caaataaact ttttgagggt ctggtccaga tggagaatgg
     4201 gtatataatc actgatgaca atatggcaac taatattcct ggagtcttcg ctgcaggaga
     4261 caacagggtt aagtcactta gacaggttgt aactgccaca gcagatggtg caattgcagc
     4321 aactcaggca gagaagtata tagagaataa tttcgaagaa taggagggat actatgatcg
     4381 aattaaacaa ggagaactat gaggaagagg tactaactgc tgaaggaaca gtgttggtgg
     4441 atttctggag tccttcatgc gagccatgca aggcgcttat gcctcatgta catgagcttg
     4501 aagaggagtt tggagacaaa atcaagttta cttcacttga cattacaaag gcaagaaggg
     4561 ttgcaattgg gcaaaaggtt cttggacttc cagtaattgc aatttaccag aatggtgaaa
     4621 gagtggacgg agtcgttgga gatgaggcta ccactaaatc cgttaaggag cttgtagaaa
     4681 aatacagcta gtccaagggg actaaaagtt tttcacttag atagcaacca aatatttcga
     4741 ggaggtgaag caaatgcgtc ttgagttagg aaagatacta ataaaagatg tagtattcgg
     4801 tgattcaacc aagcttgata atggggttct aactataaac aagtctgaat tgattgaact
     4861 tgttatggag gatgagcaca ttaaatcagt ggatgttgat attgccaagc caggtgagag
     4921 tgttagaatt acacctgtga aggacgtagt tgaaccaagg gttaaggtgg aaggacctgg
     4981 aggggtattc ccaggagtac tatcaaaggt tgacgttgta ggctctggaa agaccaacgt
     5041 actgaagggt tgttcagtta tgacaaccgg taagatagtt ggattccagg aaggtatcgt
     5101 tgatatgtgt ggtcctggtg ctgactatac tccgttttca aagctgatta acatcgttgt
     5161 taattgcgag cctgttgacg gacttaagca gcatgaccat gagaaagcag tgaggtttgc
     5221 aggctttaag tcagctgcat atattgctga aacagctaaa tcagtcacac cagatgaagt
     5281 ggaagttttt gaaactgaca catttgttga tggaatgaaa aagtatcctg accttccaaa
     5341 ggtaggatat gttcacatgc ttcagacaca gggactactt catgatacct acgtttatgg
     5401 agtggatgca aagcagattg tccctacaat tctttaccca actgagatta tggatggggc
     5461 aattgtaagt ggaaactgcg tatcagcatg tgataagaac accacctacc atcacctgaa
     5521 caatccagtt gttcatgacc tgttggcagc tcacggaaaa gagatcaatt atgttggaaa
     5581 cataatcacc aatgagaatg tttatctggc tgacaaggaa agatcatcaa actggacggc
     5641 caagttggca gaatacctta accttgatgg agttgtaata tcccaggaag gtttcggaaa
     5701 cccagataca gacctaataa tgaactgcaa gaaaattgaa gagaaggaca tcaagaccgt
     5761 aataatcact gatgagtatg ctggaagaga cggtgcaagt cagtcacttg ctgatgcgga
     5821 caagtctgca gatgcagttg ttactggtgg aaatgcaaat gaggtgataa ccctaccaaa
     5881 gatggacaag gttattggta cacttgcata tactgatatc atagcaggtg gattcgatgg
     5941 aagtttggca gatgatggaa caataacagt tgagattcag gccatcacag gtgcaacaaa
     6001 cgaaatggga ttcaacaaga tgtcagctaa aggattctag agagagcaaa acaagtaaat
     6061 aaaaaataat aaatgaaagg atgtggtaag atgtcaattt ttaacgaaaa ctcaaaagta
     6121 atcgtaattg gcgatagaga tggaattcca gggccagcca tggaagaatg cataaagacc
     6181 acacctgcag aggtagtatt ttcagcgaca gaatgttttg tctgaacggc tgcaggagca
     6241 atggatcttg agaatcagaa gagggtaaag gacctagctg agaaatacgg agcggagaac
     6301 gttgtagtat tgatcggtgg aggagaagct gaatcagcag gattggctgc agagacagtt
     6361 acagcaggag accctacatt cgcaggacca ttggcgggag ttccgttggg acttaaagta
     6421 taccacgcag tagagccaga atttaaggaa tcagtagatg cagatgtcta tgatgaccag
     6481 atcggcatga tggaaatggt gcttgatgtg gatgcaatag ttgaagaagt tgcttcaatg
     6541 agaaaagagc atggcaaata ctaaactcct aaagaataaa ggagaggagg gaatattatg
     6601 gcgaaaatta gaattgtaca ctatataaac caattcttcg ctggtattgg tggtgaagaa
     6661 aaggctgaca taaagccaga ggtcagagaa ggcgctgtag gtcctggaat ggcaatacag
     6721 gctggatttg gaggagaggc tgagattgta gccacagtaa tctgtggaga cagctatttt
     6781 ggcgaaaaca ttgaagctgc caaagaagag gttcttgaaa tggtaaaggg atacaaccct
     6841 gacctattca tcgctggacc tgccttcaac gctggaagat atggtgttgc ttgtggaaca
     6901 atcaccgagg cagttcaaaa tgaattgaat attcctgctc taacaggtat gtatattgaa
     6961 aaccctggag cagatatgta caaaaaagct gtgtatattg ttgaaaccaa gaatagtgca
     7021 gtaggaatga aggatgcagt tgcaaagatg gtaccacttg ccaagaagct actaaagggt
     7081 gaggaaattg gctcgccatc agaggaaggc tacattgaaa gaggtatcag aaagaactac
     7141 ttcactgaaa agagaggatc acaaagagct gttgacatgc ttttgaaaaa gcttaagggc
     7201 gaggaattca caactgagta tccaatgcct aattttgaca gggtggatcc aaatcctgca
     7261 gtaaaggatc tttcaaaggc aaagatagcc cttgtaacat caggtggcac tgtgcctaag
     7321 ggcaaccctg accacattga atcatcatca gcttcaaagt atgggaagta tgacattgat
     7381 ggacttaacg accttacaga agagacccat gagacagccc acggtggata cgacccagta
     7441 tatgctaatg aagactcaga cagggttctt cctgtagatg ttcttagaga ccttgaaaaa
     7501 gagggtagaa ttggtgagct tcacagatac ttctattcaa ctgttggtaa tggtacatca
     7561 gttggaagtt caaagaaatt cgctgaagag ttcagcaagg aacttattgc tgacggagtg
     7621 gacgcagtta ttctaacctc aacctgaggc acctgtacac gttgcggtgc aacgatggtt
     7681 aaggaaattg aaagagctgg aattccagtt gtccacatat gtactgtagt tccaatatcc
     7741 cttactgtag gagcaaacag gatagttcct gcaattgcaa ttcctcaccc acttggaaat
     7801 ccttccatgg acaaggtaga ggaaaaagcc cttagaagga aaata
//
LOCUS       DASRQF010000115         4853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_993488, whole genome shotgun sequence.
ACCESSION   DASRQF010000115 DASRQF010000000
VERSION     DASRQF010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993488"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VFD79_02990"
     CDS             <1..475
                     /locus_tag="VFD79_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HZH93045.1"
                     /translation="DSTAESTVKVLRSLMELIEKEPVVVQRDVPGFIGNRIQFAAFRE
                     ALHILEEGVGTPEDIDKAMKYGPGFRYPVMGPLETSDLGGLDTFYYISSYLFKSLSDG
                     KEPTEKLQELMDSGKHGVKSKEGFYDYSDGKDEEAIKRRDKMFLKMLKHIHMDDK"
     gene            complement(545..916)
                     /locus_tag="VFD79_02995"
                     /pseudo
     CDS             complement(545..916)
                     /locus_tag="VFD79_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
     gene            1069..2223
                     /locus_tag="VFD79_03000"
                     /pseudo
     CDS             1069..2223
                     /locus_tag="VFD79_03000"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
     gene            2270..3247
                     /locus_tag="VFD79_03005"
     CDS             2270..3247
                     /locus_tag="VFD79_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004819179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HZH93046.1"
                     /translation="MKNKPICYVPGREIPEVFSGVPSFLGLPVIRTEKDLEESDIVFM
                     GVPWEGVNTNGPFTSVEMATKTVRKASTRYGGYLPDFDIDVFDHFSGGDIGDVAVRNG
                     DYDFTFESIRKDYKKILDAGKFPVVFGGDHSISYPLISEFAKKYKGKIGVIHFDSHMD
                     NMDSYGEDKYARCSPFHRLYEDENVNPKNMVHYGIRGPRNNPSGLKEAKKFGAHVITG
                     MEVKINGIFESIKKAIDLASDGTEALYVTVCSDILDVAHNPAGPPDPCGLTTFELAMA
                     LHECGKAGAEAFDYVEIYPPQDPNDVSSHVASWMTIYLLSGVTKRKFNL"
     gene            3340..4290
                     /gene="glsA"
                     /locus_tag="VFD79_03010"
     CDS             3340..4290
                     /gene="glsA"
                     /locus_tag="VFD79_03010"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HZH93047.1"
                     /translation="MEILLNEIIENNRHLIEDGKVADYIPALAKADREKIGVTVAGRT
                     GALLHTAGDFEEKFTLQSISKIISFLVAAMDIGIEAIFEKVGLDSTDEPFNAFTKLDL
                     PNIHKPANPMINSGAILTTGMIKGDGEERFQKILELTRKMANNPNIGYDEEVYMSEKE
                     TGSRNRSMAYLMSSKGILDGDVEDVLDTYFKQCSIQVDTRDIAKIGRFISNGCEGINI
                     DGISRKDMSILIKGLLFTCGMYNYSAKYAIEVGIPSKSGVGGGIMGILPNGMGIGIFS
                     PALDENGNSSAGIGIIKELSRRLHFNIFDENILKAEIGPF"
     gene            4317..>4853
                     /locus_tag="VFD79_03015"
     CDS             4317..>4853
                     /locus_tag="VFD79_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZH93048.1"
                     /translation="MESGFAITDDGTKLYYRTSGEGHPLVCIHGFGADSSAYRLTEKI
                     LSRKFKVLTMDLRGHGRTITKEDSISFKSMAKDIEALLRQLDLSGVTLLGWSMGGAVS
                     LEYLRIFGEDRIRKLVLVETSPRVVNEGEWKYGLFNGDYTIETANDDLLQIQKDFMGF
                     ATDFMKRMAPHMDSENQEI"
BASE COUNT         1511 a          866 c         1129 g         1347 t
ORIGIN      
        1 tgattctact gcagaaagca ctgtgaaggt tttaaggtcc ttgatggaat taattgaaaa
       61 ggagccagtt gtggtacaaa gggatgtccc tggcttcatt ggaaacagaa tccagtttgc
      121 cgccttcaga gaggcccttc acatacttga ggagggggta ggaacaccgg aggatataga
      181 taaggccatg aaatatggtc caggcttcag ataccctgtg atgggtccct tggaaacatc
      241 tgatctgggg ggcttggaca ctttctacta catctcctct taccttttca aatcactcag
      301 tgatggaaaa gagcctacgg agaaactgca ggagcttatg gattctggca agcatggagt
      361 taaatcaaag gaaggattct acgattactc agatggtaag gatgaggaag ccataaagag
      421 aagagacaaa atgttcctta agatgcttaa acatatccac atggatgata aatagagata
      481 atcccctgaa tatactatgc tatattcagg ggatcatttt tatttaagga tgaaattact
      541 ggaactactg gatttttgtc tgggatttcc attccatgat tatctcgtct gctgctgtaa
      601 atagatcaga tagcacctcc ttctttatat catcgaggaa tgacttggaa gcgactatac
      661 tcagggtacc tagtatcttt gagggatttt ccagtatggg aactgctatg ctgctcattc
      721 ctatgcttat ctcttcgtcc tccaaagagt aaccgttgga tcttatggtc ttgatctcat
      781 ctagtaacga ctccctatca acaattgtct tgctggtata cttttcaaaa ctacttgttt
      841 tcaagtagtt ttcttgaaag tcctcgtcct gatagctgag aaaaagcttt ccagatgatg
      901 tggcattcaa ggggaagcta gtgtctaggg tatttagttt tcatttgaat tcataaaaaa
      961 actgataagg agaaatgcta tgaacaaatc cattgaatct gtacaaaaaa agtacactct
     1021 aaaggacaaa ttgaaggcta tgggacctgg gattattatt gttggatcat tcttagacct
     1081 ggaaccgtaa caacggctac aagaacaggt gcaacatttg gatttagcgt actatgggca
     1141 gttgtattct cagtcattgc aaccattgta cttcaggaga tgtccgcaag actgggaatt
     1201 ataagccaga agggattggc agaacatatc gttaacatat ttgaacataa caagtttctg
     1261 aagaacctgt ccatactgtt ggtaggagga gccataaccc tcggtggaat tgccattatg
     1321 tcaggggacc tgattggaac ttctttgggt atctccaacc ttacgggatt accaaccaac
     1381 tacatagcac ctgcaattgg agttattatt ctaattcttc tgaatgtggg tacagtaaaa
     1441 tggctggaaa agatattggg cgtccttgta ttgaccatgg tgactgtatt cgtaattaca
     1501 atgattgtag tctaacctga tcttggtgaa atggccatgg gtctggtacc aagagttccc
     1561 gaaggtgctc tgatgaatgt tgttgcactt ataggaacta cgatagttcc gtataatcta
     1621 tttattcatt gtgcaaatgc tagagacaac tggaaaaaac tagaagacct ggtcttgtcc
     1681 aggtgggata caacaatttc cataataatt ggtgggatca taacaagtgc cgtacttatt
     1741 actgcagcca cagtaatgag gggaatgact gtaaactcag ccgctgatat ggcccttcag
     1801 ctggagccta ccttgggcag gtttgcaaac atatttatga gtattggtct gtttgctgct
     1861 ggattctctt cagcgaccat aacaccacta ggagtatcat atgttctggc tggattgctt
     1921 ggctggaagc aggataaaag tgacaagaga ttcttctata caaacattgc tatactgata
     1981 ctaggtataa taggttcagc aactggattc aatccattga ccatcatcat actggctcag
     2041 gctttaaatg gtatatttct gccgcttatt gtcatattcc tcgtttatat cactgcatcc
     2101 aagaaggtgc taggaaaata ctcaaattca aaattcgcaa acgctttggg tgttatcata
     2161 agtggtataa ctcttgtaat tggaggtaga agtgtgatag atgttataaa atcaatattt
     2221 tagtgactta ataatataaa acagtttgac aatttaagga ggatgtacaa tgaaaaacaa
     2281 accaatttgc tacgtacctg gaagggaaat tccagaggtg ttcagtggag taccaagttt
     2341 tctcggactt ccggttataa gaacagaaaa ggatcttgaa gaaagcgata tcgtgttcat
     2401 gggagttcca tgggagggtg tcaacacaaa cggtcccttc acaagtgtgg aaatggcaac
     2461 caaaaccgta agaaaggcat ctaccagata tggaggctac cttcctgact ttgatataga
     2521 cgtgtttgac catttttcag gaggagacat cggggatgtt gccgttagaa acggagacta
     2581 cgatttcacc tttgaaagta taagaaagga ttacaagaaa atccttgatg cagggaaatt
     2641 tcctgtggtg tttggggggg accattcaat atcctacccc ctaatcagtg agtttgcaaa
     2701 gaaatacaaa ggcaaaatcg gagtaattca cttcgattca catatggaca atatggactc
     2761 atatggagaa gacaaatacg caaggtgttc tcctttccac aggctttatg aggatgagaa
     2821 tgtgaatcca aaaaatatgg ttcactacgg aatcaggggg ccaagaaata acccatcagg
     2881 tcttaaggag gccaagaagt tcggggctca tgtaatcact ggaatggaag taaaaatcaa
     2941 tgggatattc gaatccatca agaaggccat agatctggca agtgacggaa cagaagcatt
     3001 gtatgtaact gtttgttcag atatcctaga tgttgcccac aatccagctg gtcctcctga
     3061 tccatgtggg ttgaccactt ttgagcttgc aatggcatta catgaatgtg gaaaagctgg
     3121 tgcagaggca tttgattatg tagaaatcta tcctccacag gatcccaatg atgtttcctc
     3181 tcatgtagca agctggatga caatttatct attatcaggt gttaccaaaa ggaaattcaa
     3241 tctatagttt taaactgctg aataatgata tattatagaa taagagacca tctctatggt
     3301 ctcttattgg ctatacaata tcatagtctg gaggagaata tggaaatact actgaatgaa
     3361 ataatcgaga ataacagaca tcttattgaa gatggcaaag tagcagacta cataccagca
     3421 cttgcaaagg cggataggga gaagatagga gttacagtcg cgggaagaac aggtgccttg
     3481 ctacatactg caggggattt tgaggagaag ttcactcttc aaagtatttc aaaaataatt
     3541 tcattccttg tagctgccat ggacataggc atagaggcca tctttgagaa agttggtttg
     3601 gatagtactg atgagccctt taacgcattt acaaagcttg acctgccaaa cattcacaag
     3661 cctgccaatc ctatgataaa ttcaggtgcc atcctaacaa ctggcatgat caagggtgat
     3721 ggtgaagaga gattccaaaa aatactggag ttaaccagga aaatggccaa caatccaaat
     3781 atcggctacg acgaagaggt atacatgtct gaaaaggaaa ccggtagtag aaacagatcc
     3841 atggcatacc ttatgagcag caagggaatt ctggatggtg atgttgagga tgttctggat
     3901 acttacttta agcagtgctc aatacaggtt gatacaaggg acattgctaa aatagggaga
     3961 tttatttcca atggctgtga agggataaac attgatggta tatcaaggaa ggacatgtca
     4021 atccttatca aaggcctctt atttacctgt ggcatgtaca attacagcgc aaaatatgca
     4081 attgaggtgg gaatcccatc caagtcggga gttggaggtg gaatcatggg aatcctacca
     4141 aatggaatgg ggataggaat attctcacct gcccttgatg aaaatggcaa ctcttcagct
     4201 ggaattggta taataaagga attgtccaga agattgcact ttaatatttt tgatgaaaat
     4261 attcttaagg cggagatagg tccgttttag atatcttatg agaagttggt gatgagatgg
     4321 aaagtggatt tgcaataact gatgatggaa caaagcttta ctatcgaaca agtggtgagg
     4381 gtcatccact ggtctgcatc catggttttg gagccgacag cagtgcctac agattgacag
     4441 agaagatact ctcaaggaaa tttaaggtcc ttaccatgga cctgagaggc catgggagga
     4501 ccattaccaa ggaagacagc attagcttta aatccatggc aaaggatatt gaagcactcc
     4561 ttaggcagct tgatctgtcg ggggtcacac ttttgggatg gtccatgggt ggagcggtct
     4621 ccctggaata cctaagaatt tttggagaag atagaataag gaagttggtt cttgttgaaa
     4681 catccccaag agttgtaaat gaaggggaat ggaagtacgg tcttttcaat ggagactata
     4741 ccattgaaac ggccaatgat gacctgctcc agattcaaaa agactttatg gggtttgcca
     4801 ctgactttat gaaaagaatg gccccccaca tggatagtga aaaccaggaa ata
//
LOCUS       DASRQF010000116         4906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_832412, whole genome shotgun sequence.
ACCESSION   DASRQF010000116 DASRQF010000000
VERSION     DASRQF010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832412"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VFD79_03020"
     CDS             complement(<1..591)
                     /locus_tag="VFD79_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93049.1"
                     /translation="MNQLIRFLSIVLVAALIFLGSSILGDNTPYQGSILTPDQANLYP
                     VERIVHTGKKGDVPISLRAEYDIRGVVKGKRKYSDYPSQVSEYDLILAWGDLNREEYD
                     QHVNYSQSGRWYYYTYDQGMIDESFIASNSANVHLIPETDEIGKEIKKLRKNDYIRII
                     GYLVDVHFENGAWETSMTRGDTGNGACEIIYVTEIHV"
     gene            complement(711..1895)
                     /locus_tag="VFD79_03025"
     CDS             complement(711..1895)
                     /locus_tag="VFD79_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZH93050.1"
                     /translation="MENMKDSANVIRGLFGAMTNPGMISFGGGALAKESLPIDILREI
                     SQDILRTDSRGYEALSYGPVEGIRDLREIISTDLLGPKGVDADPDNIMILTGGLEAMN
                     LMCQLYIDPGDVILVESPTFVHSVEIFDMFQAKCVSVKMDDDGMDVEDLEEKIKIHNP
                     KMVYVIPTFQNPTGRTLNLERRKRIAELGSQYDVIILEDDPYRDIRYSGEEIPPIKSF
                     DRTGHTVLANSFSKIFSPGSRLGYILADRDIIDRVYDAKSATNSHTPMLPQILCAEFF
                     KKGHYRDHHKQLCEIHKERRDAMMEALELYFPQEAKWTYPDGGLFTWVELPEGINTTE
                     LLKESLSDPQVMVAYVAGEGFYTDGNGMGSNAMRMSFGAVPPEKIFEGIEKLGNLIKS
                     KL"
     gene            complement(1989..3065)
                     /gene="cobT"
                     /locus_tag="VFD79_03030"
     CDS             complement(1989..3065)
                     /gene="cobT"
                     /locus_tag="VFD79_03030"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF000996.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HZH93051.1"
                     /translation="MKLLSETLEKIRPADRETMERTREKWDGLVKPIGSLGLMEEMTI
                     KLSGMTGKIENKLDRKVIAVMCSDNGVLEEGVSAAPQVFTQLLAESMVKGITGVATMA
                     KFVGADIITVDMGMNGDIGIPGVIDRKIAKGTKNLAVEPAMTYEEAVRSIETGLEVGD
                     KLFSEGYDILGTGELGMGNTTTSAAMLSVLGGLDVAVTCGKGAGLTEDQYLHKMNVIK
                     RGIEVNKPDKNDPIDVLAKVGGFDIGGMCGLFLSAAKNRKPVVMDGFISSAAALCAIK
                     LNPNVADYIIPSHLSQEPGSIAMMKVLDMSPMVTMKMRLGEGSGCPLAFQILDTALFT
                     LENMGTFQEASINSEVLVDMREEQ"
     gene            complement(3083..3337)
                     /locus_tag="VFD79_03035"
     CDS             complement(3083..3337)
                     /locus_tag="VFD79_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HZH93052.1"
                     /translation="MENSYRFYRNTDCQYFPCHKVQDEKEFNCMFCYCPLYLMEDCGG
                     KFKDVNGIKDCTNCIIPHRPKGYDYINMKIMENNSQGRAK"
     gene            complement(3345..4082)
                     /gene="cobK"
                     /locus_tag="VFD79_03040"
     CDS             complement(3345..4082)
                     /gene="cobK"
                     /locus_tag="VFD79_03040"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF014613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HZH93053.1"
                     /translation="MIWVIGGTSEAREFINLFENREILIVTVATEDGRRVLSGANIIT
                     GRLDFQSMIHFIREHRIRILVDLSHPYAIEVSNNAEMAAAAEGIPYLGFSRGKTEWFK
                     EAIYLDSFDSCLEKVREMEGTFFFTTGVKTLPDFEKIRGSNRFVHRIIPSQESLGICL
                     DNKVGLNNIVALLGPFSRELNRELFKEYEVDYVVMKDSGIRGGTKEKLLACRDLGIVP
                     LVIGRQEKEGYTDLVKMKLETIGYYKE"
     gene            complement(4079..4804)
                     /gene="cobJ"
                     /locus_tag="VFD79_03045"
     CDS             complement(4079..4804)
                     /gene="cobJ"
                     /locus_tag="VFD79_03045"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HZH93054.1"
                     /translation="MFGKVFVVGIGPGGRDNMTFRADKALSDCHAVVGYSGYLKYVED
                     YLPGKEVYSTGMKGEIDRCRMAIQLAKEGMDVAVVSTGDAGLYGMAGPILEMSEELDV
                     EIIPGISACFSAASDLGSPIMHDFVTISLSDLLTPWETIEKRIKLAAEGDFVISLYNP
                     RSKGRPDYLKKAVDLILQFRGGGTPVGIVRNSGREDTEKKVRTLASIDYEEVDMMSVL
                     IIGNSTTYIRNEKIITPRGYDIL"
BASE COUNT         1198 a         1308 c          897 g         1503 t
ORIGIN      
        1 aacgtgaatc tcagtgacat atattatttc acaagctccg ttgcctgtat cccccctggt
       61 catgctggtt tcccaggctc cattctcaaa atgcacatcc actagatatc caatgattct
      121 gatgtagtca ttctttcgaa gtttttttat ctcttttcca atttcatcgg tttctggaat
      181 aagatggaca tttgcggaat tgctggctat gaagctttca tcaatcatgc cctgatcata
      241 ggtatagtag taccatctac cactttggga ataattaaca tgctggtcat actcctccct
      301 gttcaggtca ccccaggcaa ggataagatc gtattctgat acctgtgatg ggtaatccga
      361 gtattttctc tttcctttaa ccactcccct gatgtcgtac tcagccctta atgatatggg
      421 aacatcccct ttctttccag tatggactat cctttcaact gggtagagat tagcctggtc
      481 aggcgttaga atgcttccct gatagggggt gttgtcacct agtatggagc ttccaagaaa
      541 tatcaaagca gcaaccaata ctattgatag aaacctgatc agctgattca tacaactatc
      601 ctcccattga tttttgatac taatagtata ccttattgaa gtctttttgt gtagattaca
      661 actaaaaaag gacctgtcca ttaacaggtc ccttctatag cctacttaaa ctacagcttt
      721 gactttataa gattaccaag cttttcaata ccttcgaata tcttctcagg cggcacggca
      781 ccaaagctca ttctcatagc gttgctgccc attccattcc cgtctgtata gaagccttca
      841 ccggcaacat atgcaaccat tacctgggga tcacttaggg attccttcaa aagctcagtt
      901 gtatttattc cttccggcaa ttccacccag gtaaatagtc caccatctgg ataggtccat
      961 tttgcctcct gggggaaata gagctccaga gcttccatca ttgcatcccg tctttccttg
     1021 tgtatctcac agagttgttt gtgatggtcc ctgtagtggc ctttcttgaa gaactccgca
     1081 cagagaatct gtggaagcat tggggtgtgt gaattggtgg ctgattttgc atcgtatacc
     1141 ctatctatta tatccctgtc cgccaagata tatcccagtc tgcttccagg agagaatatc
     1201 ttggaaaagc tgttggcaag gactgtatgt ccggtcctgt caaaggattt tattggtggg
     1261 atctcctctc cactgtatct tatgtccctg taggggtcat cctcaaggat tatgacatca
     1321 tactggcttc ccagctctgc aatccttttt cttctttcaa ggttcagggt tctaccagtt
     1381 gggttctgga aggttggaat cacatagacc atctttggat tatggatctt tatcttctct
     1441 tcaagatcct caacatccat gccgtcatca tccatcttca cggacacgca cttggcctgg
     1501 aacatgtcaa atatctccac actgtgtaca aaggttggag actccacaag aatcacatca
     1561 cctggatcga tgtaaagctg gcacataagg ttcatggcct caagaccgcc tgtcagtatc
     1621 ataatattgt cagggtcggc atccacaccc ttgggaccaa gcaggtcagt tgagattatc
     1681 tccctaaggt ccctgatccc ttctacaggc ccgtaggaaa gagcttcata acctcttgaa
     1741 tcggtcctta atatatcctg ggatatttcc ctaaggatat cgataggaag ggattccttt
     1801 gcaagggctc ctcccccgaa ggatatcatg ccaggatttg tcatggcccc gaaaagaccc
     1861 cttattacat ttgctgaatc cttcatgttt tccattctat ttgagaactt aaccatagca
     1921 cttcaccact ttcaatagtt tattaagtga tgatacacca tttccacgtg aaaagcaata
     1981 ggtcccccct attgctcctc cctcatgtca acaaggacct cactgttgat tgaagcctcc
     2041 tggaaggttc ccatgttttc aagggtaaac agggcggtat ccagaatctg aaaagccaac
     2101 ggacagccag acccctctcc tagtctcatt ttcatggtta ccattggcga catgtcaagc
     2161 accttcatca ttgcaattga gcccggttcc tgtgaaaggt gggatgggat gatatagtcg
     2221 gccacatttg gatttagttt gattgcacaa agtgctgccg ccgaagatat gaagccgtcc
     2281 attacaacgg gctttctgtt ttttgcagcc gaaaggaaga gtccgcacat tcctccgata
     2341 tcaaaacctc caacctttgc aagaacatca attggatcat ttttatctgg tttgttgacc
     2401 tcgattcccc tctttatgac attcatctta tggagatact ggtcctcggt caaacctgcc
     2461 cctttgccgc aggtgactgc aacatcaagg cctcccaaaa cactaagcat ggctgcagat
     2521 gtggtggtgt ttcccatccc aagctctcct gtccccagga tgtcatagcc ctcactaaac
     2581 agcttgtctc caacctctaa cccagtttct attgacctaa cagcctcttc ataggtcatg
     2641 gcaggttcaa cagccaggtt ttttgtcccc ttggcgatct tacggtcgat tactcccggt
     2701 atgcctatgt cgccattcat tcccatatcc actgttataa tgtctgctcc aacaaatttt
     2761 gccattgtgg caactcctgt tatccccttt accatgctct ctgcaagtag ctgggtgaac
     2821 acctggggag ctgcacttac gccctcttcc aggactccgt tgtcactgca cataacggct
     2881 ataaccttcc tatccagttt gttttctatc tttcccgtca ttcccgaaag tttgatggtc
     2941 atttcctcca tcagtcccag gcttcctatg ggtttcacaa ggccatccca tttctccctt
     3001 gtcctttcca tggtttccct gtctgcaggt cttattttct ctaacgtttc acttaatagt
     3061 ttcatccttc caccccttat cattatttgg cccttccctg actattattc tccattattt
     3121 tcatatttat gtaatcatat ccctttggcc tatggggaat aatgcaatta gtgcaatcct
     3181 ttatgccatt cacatccttg aatttacctc cacaatcctc catcagatac aggggacagt
     3241 agcagaacat gcaattgaac tccttctcat cctgtacctt gtggcatgga aagtactgac
     3301 aatccgtgtt tctatagaat ctgtacgagt tttccacagc ttgttcactc cttgtaatat
     3361 ccaattgttt ccaacttcat cttcaccagg tctgtgtagc cttccttctc ctgcctgcca
     3421 ataaccagag ggacaatacc aaggtccctg caggcaagga gcttctcctt tgtcccgcct
     3481 cttattccgc tatccttcat cacaacataa tcgacttcat actccttgaa cagttccctg
     3541 ttcaattccc ttgaaaaggg accaagcagt gcaacaatgt tattaagacc aaccttattg
     3601 tccaaacata ttccaagact ttcctgggag ggtattatcc tgtgaacaaa tctgttacta
     3661 cctctgatct tttcaaagtc aggaagtgtc ttgacccctg tggtgaaaaa gaaggtcccc
     3721 tccatctctc tgactttttc taagcatgaa tcaaatgaat cgaggtatat ggcttcctta
     3781 aaccattccg tctttcccct tgaaaagccc aggtatggta tcccctcagc tgctgcagcc
     3841 atctcggcat tgtttgaaac ctcaatagca tatgggtgtg aaaggtcaac taaaatcctt
     3901 atcctgtgtt ccctgatgaa gtggatcatg ctctggaaat caagtcttcc tgtgataatg
     3961 tttgctcccg aaagaactcg tctgccgtct tcagttgcta cagttacaat aaggatctcc
     4021 ctattctcaa ataggtttat gaactccctg gcttctgatg tccctccaat cacccatatc
     4081 atagtatatc ataacccctt ggtgttatta tcttttcatt tcggatatat gtggtgctat
     4141 tgcctattat tagaacactc atcatgtcaa cctcttcata gtcaatgctt gcaagggtcc
     4201 gaaccttctt ttcagtgtct tccctgccgg aatttctgac tatgccaaca ggtgtccctc
     4261 cacccctgaa ctggagaatc aagtccactg ccttttttag gtaatccggc ctcccctttg
     4321 atcttggatt gtagagggaa atgacaaaat ccccttccgc tgcaagtttt atcctttttt
     4381 ctatggtttc ccagggtgtc agcaggtcac taagacttat tgttacaaaa tcatgcataa
     4441 tcggacttcc aaggtctgaa gctgctgaga agcaagcgct gatcccgggt atgatctcaa
     4501 catccaattc ctctgacatt tcaagaatgg gtcctgccat accatagagg ccagcatcac
     4561 ccgtgctcac aacggcaaca tccattcctt ccttggcaag ctggatcgcc atcctgcacc
     4621 tgtcaatttc acccttcata ccagttgaat acacctcctt gcccggtagg taatcctcaa
     4681 catactttag gtatcctgaa tagccaacaa ccgcatgaca atcacttaaa gccttatctg
     4741 ccctaaaagt catattgtcc ctgccaccag gtccgattcc aacaacaaaa accttaccaa
     4801 acatccctac acctccactg taaccgacaa ggtgatcccg ttttttacgg ccttctccct
     4861 tatcaggttt cctcccataa gatatgctgc aggtgccgat accgag
//
LOCUS       DASRQF010000117         6643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_534377, whole genome shotgun sequence.
ACCESSION   DASRQF010000117 DASRQF010000000
VERSION     DASRQF010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534377"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4754)
                     /locus_tag="VFD79_03050"
     CDS             complement(<1..4754)
                     /locus_tag="VFD79_03050"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZH93055.1"
                     /translation="MKLLNDNIRLIEKFDSKNTEKAYVCELLDKTGQESFVRILDGNQ
                     HKQLIQDYIKNIDFYKSINHKGILSTIDFGLVKTLDFKESAPDMYYILSEYTPWRTLD
                     AVMDGLSIHSRISILLKIIEALDYIHYRGITYDVLSPDKIFVSQEGDVKLLNIASTVH
                     YKLQMENLKKTSDNMEHDGRWKSNGKTPQADFWSLGIVAEKLFSGIRSDLSLEKQGVL
                     GEIIEDLMTKGKDANRKSLANHLDALVSVFDIDYPRDLRREREILQLSVLPKRNSQSF
                     RRFLPSSSFDELYEKGYSGLIVEGNGGIGKKKYIHELFRRRSLEGSRTYLLEAGQGDF
                     SSIDHFKAFIVQLCEALLIQYKIDPESNNMKIRFKDTFEVFRLDQLDSKLKLFNGITE
                     EFVGLSKRKHVFFGLLNIHDADIEIFNLMDFIITKSRGKRIHFLFSLQNNQLSDTRKI
                     IIVNRWLKNNLLDKIRLENLSKDEAEDYIHEILGNTKAPKAWMDELYNETLGNPRYIR
                     VLVKHFFDNGVICIGRNGFWTTTTDDYRAFYNSAALKRTILRQIKTLDEMELKVLGIL
                     SCFNHPANLTTLINVLEVDGTDIGELIGQLTHKGIIKIQRHPETVVSFVDGDFKRQVY
                     SDLMDEDRVKLHTRIADAILNNREDSHAINFDSLIYHLSESSQLDKMVELGLQRLENE
                     GNRYNENSVNILSTCYGNLKGRDHPLTLMILKYLTEALLAQRRYTEGIEFSKSYGDLA
                     RKLGDSGHEIHADLFELEFIIRSGDYTEALEKITEYEEIAARPGNQVHRINLYKLEAI
                     IHQVLDRLEESQGAIDRALELSHKHGIHQHDGDLYNLQGIGNYLNGDHKKALESYQRS
                     LDTYVLSEREFDRIKPLNNIGNLYNEIIGDPQEALKYYFECLRVSDENGLSSFQTIAL
                     SNICDVYLTLRSYEDARTYIERTIELSHINGDRIIEFHGNVYKGVLELSLNNIEAASE
                     IFLKIRELNKEDPILEKEVKVHYMDFLGRFYMELGDYNLGKMFTRNTMEKSEGFNPKL
                     YFRSKSRMLTIDAIMNKRINQGEIGDLFKEWESGGNDYEKAAFLVEMMHISLHIPEKQ
                     GFAFLKKLYLGLKTRSAWDLFRNDFKVMNILVRNGTRYLGKAIDLLEVESKGFSQSIC
                     RYYAYVGELLYEVGDCKGAARYLIMSMDLVQTRINQVGIDGYREKMLNNYNIPKITSI
                     LSEIFKKEYGMELGESGPQAHESSLVGGYLTRLGYEQYNELFLNVSGEDIPGTFEKIL
                     SAMTSDHKFNLELMLKYLSREMGAKTSLINIYRFEESDSDSSLIYLGEKRLEIDQFVD
                     KRLRSGENVVFNRDQDILPVGASKEYFDKSIVALMGVPVTEPKRDDLHEDRRSGIRKS
                     RVLGYVYLETDSTINRFDEKRLALAMGMAKLIYLNLENRKLNRRANFDRLTNVLSRES
                     IENEMEEVMDLYSGTEGEFAVLMMDIDKFKDINDTYGHQKGDHVLRIIGEVLNENTRS
                     SDHVGRYGGEEFLILLENISMEDAESVAEEIRKAVQADRRYAVDRKVTISIGISHYPN
                     HGTTKEDLIYKADQSLYYAKEVLGRNSVAMWD"
     gene            complement(4770..5213)
                     /locus_tag="VFD79_03055"
     CDS             complement(4770..5213)
                     /locus_tag="VFD79_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZH93056.1"
                     /translation="MTHKKSEHVDSIEEYLRKVDYIIRRKGRVILSDFNITVPQFTAL
                     QILINNGEMTISELSQNMDLACSTITDLIDRMEKTNLVRRKKDDQDKRVVRVEVLQEG
                     HDILARVLEKRIEFLDDKLRGVTEEDKQTLSTALKNLYMGMKEEV"
     gene            complement(5224..6108)
                     /locus_tag="VFD79_03060"
     CDS             complement(5224..6108)
                     /locus_tag="VFD79_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HZH93057.1"
                     /translation="MKKIKFIINPSSGRQAMEKRIDNLCKMLLDDGYIVGKSFTKKKY
                     DAMFEAREACKEGFDLIVACGGDGTVNEVVKGIMTSDNKVPLGILASGTVNDFANYLQ
                     IPRTSEEFFNMIKKENIIEVDLGKVNDDYFVNVAAGGLLTKVGYQVPSDMKAVLGRMA
                     YYLEGLRELVSQGLEPVKVKITSEEHTGEEEILMFVVSNSSSIGGFKRLAPEADVLDG
                     LLDVVLIKNADIAELANIFLNVLTGEHIRHPKVVYYKTREVSMDAEDALVVDVDGEYG
                     GKLPAHFKVIPKGLKILV"
     gene            complement(6236..>6643)
                     /locus_tag="VFD79_03065"
     CDS             complement(6236..>6643)
                     /locus_tag="VFD79_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93058.1"
                     /translation="LGYLLLILRNVNKVLGQAETVLEANMNEIDATIKQLPEITENVN
                     KITNEANKMISDMSPDLTGVVRNVNGITTKIDDMANVIDNTGHKVGDTVDVLSETLAE
                     TALAFQFNAKSVTSYIEIIKEIIEIVRDSIKKY"
BASE COUNT         1684 a         1608 c         1139 g         2212 t
ORIGIN      
        1 ctatcccaca tggccacact atttctgcca agtacttcct ttgcataata gagggattga
       61 tcggctttgt aaatcaggtc ttccttggtg gttccatgat tcgggtagtg tgatatgcca
      121 atgcttattg tcacctttct atcgactgca taccttctgt ctgcttggac tgccttcctt
      181 atctcctccg ctactgattc tgcatcttcc atggagatat tttccagcag aatcaagaat
      241 tcctctcctc cgtatcttcc cacatggtcg cttgatctgg tgttctcgtt taatacttct
      301 ccaattatcc ttagtacatg gtcacccttc tgatgaccat aggtatcatt tatatccttg
      361 aacttatcaa tatccatcat taaaacagcg aactcaccct cagtcccact gtacaggtcc
      421 attacttcct ccatctcatt ttcaatggat tcacggctga gtacattggt taacctatca
      481 aaattagccc ttctatttaa tttccggttt tccaggttga gatagatcag ctttgccata
      541 cccattgcca gagcaagtct cttctcatca aacctattga ttgtactgtc tgtctcaagg
      601 taaacatagc ccaggaccct gctttttctg atgcccgacc ttctatcttc atgaaggtca
      661 tctcttttgg gctcagttac aggaaccccc atcaaagcga ctatgctctt atcaaagtat
      721 tccttgcttg cacccactgg taggatatcc tggtccctat tgaaaacaac attttctcct
      781 gaccttaaac gcttgtcgac aaactggtct atctcaagtc ttttttctcc caggtaaatt
      841 aaacttgagt cactgtcgct ctcctcaaac ctgtagatat tgatcaatga agtctttgct
      901 cccatttccc ggctaaggta tttgagcata agctccaggt tgaacttatg gtcacttgtc
      961 attgcagata agattttttc aaaggtacca ggtatatcct cgccacttac attgagaaac
     1021 agctcattgt attgttcata tccaagcctt gtcaggtagc ctcccacaag acttgactca
     1081 tgggcttggg gacccgactc cccaagttcc ataccatact cctttttgaa aatttcactt
     1141 aggatgcttg ttatctttgg tatgttgtaa ttgttgagca tcttttccct gtatccatct
     1201 attcctacct gattgattct ggtctggacc aaatccatgg acatgatcag atatctggca
     1261 gcccctttac aatctccgac ttcatacaaa agctctccca cataggcata gtatctgcaa
     1321 atggactgtg agaatccctt gctctcgact tccaacaggt ctatggcttt ccccaaatat
     1381 ctagtcccat ttctcacaag tatgttcatc actttgaagt cattcctgaa caggtcccaa
     1441 gcacttcttg tctttagccc aaggtaaagc ttcttaagaa aagcaaagcc ttgcttttct
     1501 gggatgtgaa ggctgatgtg catcatctct accagaaagg ccgctttttc gtagtcattg
     1561 ccacctgatt cccattcttt gaataaatcc ccaatttcac cctgattaat tctcttattc
     1621 atgattgcat ctattgtcag cattcttgac tttgatctga agtatagctt gggattgaaa
     1681 ccctctgact tttccattgt gttcctggtg aacatcttcc ccaggttgta gtctccaagc
     1741 tccatataga accgacccaa aaaatccata taatgaactt taacctcttt ttccaaaatg
     1801 gggtcttcct tgttcagttc tcttatcttg agaaatatct cgctagctgc ttctatgttg
     1861 ttcagtgaaa gctcaagaac ccctttatat acattaccat ggaactcgat gatcctgtct
     1921 ccattaatgt gggatagttc aatggtcctt tcaatgtagg ttcttgcatc ttcatatgac
     1981 cttagagtga gatacacatc acaaatgtta ctcaatgcaa ttgtctgaaa tgatgatagt
     2041 ccgttttcat ctgaaaccct cagacattca aaataatact taagggcttc ctgggggtct
     2101 ccaattattt cattataaag atttccaatg ttgtttagtg gtttaatcct atcaaattcc
     2161 ctctcagaga gaacgtatgt gtcaagtgag cgttgatagc tttccaatgc ctttttatgg
     2221 tctccattca gataattacc tattccctga agattataaa ggtcaccgtc atgttggtga
     2281 attccatgct tatgggataa ttccaaagct ctgtctattg caccctggga ctcctccagt
     2341 ctatccaaga cctgatgtat tattgcctct agcttgtaca ggttaattcg atgcacctgg
     2401 tttccaggcc tagctgcaat ttcttcatat tcagttatct tttcaagagc ctcagtgtag
     2461 tctccagaac ggataatgaa ctcaagctca aacaggtctg cgtgaatttc atgtccagag
     2521 tcacctagtt ttcttgccag atcaccgtat gactttgaaa actcaatacc ttcagtgtat
     2581 cttctttgag ccagcagagc ctcggtcagg tatttcagta tcatcagggt taagggatgg
     2641 tccctgccct tcaaattccc ataacaggtg cttagaatgt ttaccgagtt ttcattatac
     2701 cggttgcctt cattttctag cctctgcaat cctagttcaa ccattttatc cagttggctt
     2761 gactcactta aatgatatat cagggagtca aaattgatgg catggctatc ctctctattg
     2821 tttaggatcg catcagcaat cctggtgtga agcttgaccc tatcctcatc catcaggtcg
     2881 ctgtagacct gtctcttgaa atcaccatcc acaaaggata ccactgtctc tggatgtctc
     2941 tggattttga ttatcccctt atgggtaagc tgaccgataa gttccccaat gtccgtccca
     3001 tcaacttcaa gtacattgat taaggtcgtc aggttggcag gatgattgaa gcaggataat
     3061 atgccaagca ccttcaactc catctcatcc agggttttaa tctgccttaa aatggtcctt
     3121 ttcaaggctg cagaattata gaaagccctg taatcatctg ttgtggtagt ccaaaatcca
     3181 ttcctgccaa tacatatgac accattatca aaaaaatgtt ttaccagaac ccttatataa
     3241 cgggggtttc ccaaggtttc attgtagagt tcatccatcc atgcctttgg tgccttggtg
     3301 tttccaagaa tttcatgaat gtagtcctca gcttcatcct tggaaaggtt ctcaaggcga
     3361 atcttatcca ataggttgtt cttaagccat cggtttacta tgataatctt ccttgtgtca
     3421 gacaattgat tgttttgaag ggaaaacagg aaatgtatcc tctttcctct agactttgta
     3481 ataataaaat ccatcaggtt gaaaatctca atatcagcat cgtgtatgtt cagcaagccg
     3541 aaaaatacat gtttcctttt ggaaagtcca acgaactctt cagttatgcc attgaacagc
     3601 ttgagctttg aatccaactg gtccagtctg aagacctcaa aagtatcctt aaacctaatt
     3661 ttcatattgt tggattctgg atcaatctta tattgaataa gaagggcttc gcataattga
     3721 acaatgaatg ccttgaaatg gtctatgctt gaaaagtcac cctgtccagc ttcaagcaga
     3781 tatgtcctgc tgccctcaag acttcttctt ctgaaaagct catggatgta tttcttcttg
     3841 ccgattcctc cattgccttc aactatcaat cctgaatagc ccttctcgta tagttcatca
     3901 aatgagctac tgggaagaaa tctcctgaag ctctgtgaat ttctttttgg caaaacagat
     3961 aattgcaaaa tttctctttc tcttctgagg tcccttggat aatcaatgtc aaatacacta
     4021 accaaggcat ccaagtggtt agccaaggat tttctatttg catccttgcc ttttgtcatc
     4081 agatcttcga taatctcccc taatacaccc tgcttctcaa gagacaagtc tgatcttatc
     4141 cctgagaaca acttttccgc aacaatccca agggaccaga aatccgcttg tggggttttc
     4201 ccattgcttt tccacctgcc atcatgctcc atgttgtcac tggttttttt cagattttcc
     4261 atttgcagct tgtaatgtac agtggatgca atatttagaa gcttcacgtc cccttcctga
     4321 ctgacaaata tcttgtcagg acttaacaca tcataggtaa tgcccctata atgtatgtag
     4381 tccagggctt caataatctt caggaggatt gaaatacgag aatggatgct caatccatcc
     4441 atgacagcat caagggttct ccagggtgta tattctgaaa ggatgtagta catatctgga
     4501 gcagactcct tgaaatccag tgttttaacc agaccaaaat caattgttga caggataccc
     4561 ttgtggttta tactcttata gaagtcgata tttttgatat aatcctgaat cagctgttta
     4621 tgttgattcc catccaggat tcgtacaaag ctctcttgtc cagttttgtc caaaagttca
     4681 cacacataag ccttttccgt gtttttggag tcaaactttt caatcagtct tatattgtca
     4741 tttaaaagct tcatgtggac cctcccttgc tagacttctt ccttcatccc catataaagg
     4801 tttttcaagg ctgtactcaa tgtctgtttg tcctcttctg ttactcccct tagcttgtca
     4861 tcgagaaact ctatcctctt ttccagtacc cttgcaagaa tatcatgccc ttcctggagc
     4921 acttccaccc ttacaacccg tttgtcctgg tcgtcttttt ttcttctgac aaggtttgtc
     4981 ttctccatcc tgtcaatcag atcagttatg gtactacagg caaggtccat attctgactg
     5041 agttcactta tggtcatctc tccattgttt atcagtatct gcaaggcagt gaactgggga
     5101 acggttatat tgaaatcact tagtattacc cttccctttc ttctgattat ataatctacc
     5161 tttctcaggt actcttctat ggaatcaacg tgttccgact ttttatgtgt cattggcaac
     5221 ctcctaaacc agtattttca atccttttgg tatcacctta aagtgggctg gcagctttcc
     5281 gccatattct ccatcaacat caacaaccag ggcatcctcg gcatccatac taacttcccg
     5341 ggttttgtag tagactacct tggggtgcct aatgtgttct cctgtaagta cgttgaggaa
     5401 aatatttgcc aactcggcaa tatcggcatt ctttattagg acaacatcca gcaggccatc
     5461 aagaacatct gcttcagggg ccaatctttt gaatccaccg atggatgaac tgtttgaaac
     5521 aacaaacatc agtatctctt cctccccagt atgctcttca cttgtgatct ttacctttac
     5581 aggctcaaga ccctggctca caagctccct caggccttcc aggtaatagg ccatcctccc
     5641 caatacagcc ttcatatctg aaggcacctg gtatcctact tttgttaaga gtcctcctgc
     5701 tgcaacgttt acaaagtagt catcattaac cttgccaaga tccacttcaa tgatgttctc
     5761 ctttttaatc atgttgaaga attcttctga agttctgggg atctggagat agttggcaaa
     5821 atcgttaact gtcccactgg ccaaaatccc caagggtacc ttgttgtcgc tggtcattat
     5881 tcccttgacc acctcattta cagttccgtc tccaccgcag gcaactatca ggtcaaaacc
     5941 ctccttacag gcctccctgg cttcaaacat cgcgtcatac tttttctttg tgaaggattt
     6001 gcctacaatg tagccatcat ccaatagcat cttgcatagg ttgtcaattc ttttctccat
     6061 ggcttgtctg ccagaggagg gatttataat gaacttaatc tttttcatac ttattctcca
     6121 ttccgtgctt tgtcatatgt ttatttatgg gcatatcact ttattatatt ataccagtta
     6181 tctgccactt gtcctagtta tttaaacaag aaaaaaagag ccccttcggg ctcaattagt
     6241 actttttaat gctatctcgc actatttcaa ttatctcttt gataatctca atgtacgatg
     6301 taactgattt ggcattgaac tgaaatgcca atgccgtctc cgccaatgtc tctgatagta
     6361 catccacagt gtctccaacc ttgtggccag tattatcgat tacattagcc atgtcatcaa
     6421 tcttggtggt tattccattt acattcctaa ccacacctgt caagtcaggt gacatgtctg
     6481 agatcatttt atttgcttca ttggtgatct tgttaacatt ctcggtaatt tcaggcagtt
     6541 gcttaattgt cgcatcaatt tcattcatgt tagcttccaa aacggtttcc gcttgaccga
     6601 gaaccttatt gacattcctt aaaataagaa gtaagtatcc aag
//
LOCUS       DASRQF010000118         3806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_862817, whole genome shotgun sequence.
ACCESSION   DASRQF010000118 DASRQF010000000
VERSION     DASRQF010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862817"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..1755
                     /gene="ftsH"
                     /locus_tag="VFD79_03070"
     CDS             7..1755
                     /gene="ftsH"
                     /locus_tag="VFD79_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HZH93059.1"
                     /translation="MFGTEVEQVTELDFTELVSKINDGSITRITSIGNTVEGELEDGS
                     KFSGFLPEEARLTFYPDYVKELVDNQEIIYDGKPIPETPWYINMLPTIMILVVFAVFW
                     FVFMQQSQGGGGGGKVMSFGKSKAKLVKMEEGKLVSFDEVAGLEEEKEELQEIVDFLS
                     NPKKYIEIGARIPTGVLMVGPPGTGKTYLSKAVAGEAGVPFFTISGSDFVEMFVGVGA
                     SRVRDLFEQAKKNSPCIVFIDEIDAVGRRRGAGLGGGHDEREQTLNQLLVEMDGFAKN
                     EGIIVMAATNRADILDPALLRPGRFDRTIYVGLPDIRGREAILKVHTRKKPLEEDVDL
                     GVVAKRTPGFTPADLENLVNEAALLTARFNLKKIPMHLIEEASIKVQAGPEKKSRVIS
                     PHEKKLTAFHEAGHALTARLLPGSDPVHLVTIIPRGMAGGFTAYIPDEDRNYLTKNQM
                     QDKLVILLGGRVTEALVLEDISTGAQNDIERATKLARSMVTHYGMSDVLGPMTYGTDE
                     EEVFVGRDLNRSRNYSEDVAAAIDKEMRKIIDTAYHRAESLLKDNMEVLHRIAEALLE
                     KETIDAREFEELFQGN"
     gene            1916..3595
                     /locus_tag="VFD79_03075"
     CDS             1916..3595
                     /locus_tag="VFD79_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HZH93060.1"
                     /translation="MYDEKKLEQIKSSFVDWDENKVEKQLGKHPERKETFTTGSDKKV
                     ERLYTPLNTGEMDYERDLGYPGEYPYTRGVQPTMYRGRFWTMRQYAGFATAEESNKRY
                     KYLLDQGQTGLSVAFDLPTQIGYDSDHGLSEGEVGKVGVAIDSLRDMEILFDGIPLDK
                     VSTSMTINAPASVLLAMYIAVAEKQGVSPDKLRGTIQNDILKEYIARGTYIFPVEESM
                     RLITNIFEYCSKEVPQWNTISISGYHIREAGSTAAQEVAFTLADGIAYVDAAIKAGLD
                     VDDFAPRLSFFFNAHNDLLEEVSKYRAARRLWARIMKERFGAKKEKSLQLKFHTQTAG
                     STLTAQQPDNNIVRVAIQTLAAVLGGTQSLHTNSRDEALALPTEDSVRIALRTQQIVA
                     HESGVTETVDPLAGSYYIESLTNDIEKEAMDYIEKIDNLGGAPKAIEKGFIQKEIQNS
                     AYKYQMQVEDQERIVVGVNKFRIDETEKKELLKVDPHVELLQKKKIEKLKSTRDNDKV
                     QGALELLKAKAETDENLMPFIIEAVKEYATLGEVCGVLREVFGEYEQSVIL"
     gene            3614..>3806
                     /locus_tag="VFD79_03080"
     CDS             3614..>3806
                     /locus_tag="VFD79_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309684.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HZH93061.1"
                     /translation="MDKPIRVLVAKPGLDGHDRGAKVIARALRDAGMEVIYTGLRQTP
                     EQIVAAAVQEDVDVVAMSIL"
BASE COUNT         1268 a          690 c          984 g          864 t
ORIGIN      
        1 gtaaggatgt ttgggacaga ggtagagcag gtaacagagc tggatttcac agagctggtg
       61 tcaaaaatta acgacggaag catcacaaga ataacttcaa tcggaaacac tgttgaggga
      121 gaactggagg atggcagtaa attttcagga tttttacctg aagaggctag actgacattc
      181 taccctgatt atgtaaaaga actggtggac aatcaagaga ttatatatga tggaaaaccc
      241 attcccgaaa caccttggta tataaatatg ctgcctacaa tcatgatatt ggtagtattc
      301 gcagtcttct ggtttgtttt catgcaacaa tcccagggcg gcggaggcgg cggaaaagtc
      361 atgtcctttg gaaagagcaa ggcaaagctt gtcaagatgg aagaaggaaa gctggtctcc
      421 tttgacgagg ttgccggact tgaggaggag aaggaggagc tgcaggaaat tgtagacttc
      481 ctgagcaatc caaagaaata cattgaaatt ggagccagaa taccaacagg agtcctaatg
      541 gtaggacctc ctggaacagg taaaacctat ttgagtaagg cagtagcagg tgaagcgggt
      601 gtacctttct ttacaatctc cggttcagac tttgttgaaa tgtttgtcgg tgtgggtgca
      661 agccgtgtaa gggacctgtt tgaacaggcc aagaagaatt caccatgtat agtattcata
      721 gatgagatcg atgcagtcgg tcgaagaaga ggcgcaggtc ttggtggtgg ccatgatgag
      781 agggagcaga cattgaacca gcttcttgtt gagatggatg gctttgccaa gaatgaggga
      841 ataatagtaa tggctgcaac aaacagggca gacattctgg accctgctct attaaggcca
      901 ggcagatttg acaggacaat atatgtagga cttcctgaca tcagaggaag ggaagccata
      961 ctaaaggttc acacaaggaa gaaacctctt gaggaggatg ttgaccttgg agtggttgca
     1021 aagagaacac caggctttac acctgcagac cttgagaacc tggtaaacga ggcggcctta
     1081 cttactgcaa ggttcaatct caagaagata cccatgcacc tgattgaaga ggcatccata
     1141 aaggtccagg caggaccgga gaagaagagc agggtcatta gcccacatga aaagaagctg
     1201 acagcctttc atgaggctgg gcatgcactt actgcaagat tgttgccggg aagcgacccg
     1261 gtccacctgg ttacaataat accaagaggt atggctggtg gattcactgc atatattcct
     1321 gacgaggaca gaaattatct gaccaagaat cagatgcagg acaaacttgt aatactactg
     1381 ggaggaaggg ttacagaagc ccttgtcctt gaggacataa gtacaggagc tcaaaacgat
     1441 attgaaaggg caactaaact tgccagatca atggtaactc actatggtat gagtgatgtc
     1501 cttggaccca tgacctatgg tacagacgaa gaggaagtat ttgttggaag agaccttaac
     1561 agatccagaa actatagtga ggatgttgcc gcagctatag acaaggagat gaggaagata
     1621 atcgatacag cttaccacag ggcagagtca ctattaaaag ataatatgga agtgcttcat
     1681 aggattgcag aggcgttact tgaaaaagaa acaattgatg caagggaatt tgaagaacta
     1741 ttccaaggaa actgatgcag gaccccttat tagggggcct gcttttccca tgtcttgaca
     1801 taaaaatgca attttacatt cattattcaa aataattgaa ttttatattg caaaattgca
     1861 acaacttcta ctataatagg ggttagagaa ggtaccaata cgaggaggta aaatgatgta
     1921 cgatgagaaa aagctagagc agattaagtc gtcttttgtg gactgggatg aaaacaaagt
     1981 cgaaaagcag cttggcaagc atcctgaaag aaaagaaacc tttacaaccg gttcagacaa
     2041 gaaggtggaa aggctttaca caccactaaa cactggagaa atggattatg aacgggatct
     2101 tggctatcct ggagaatatc catacactag aggtgtacag ccaacaatgt acaggggaag
     2161 attctggaca atgagacagt atgcgggatt tgcaactgcg gaagagtcca acaagagata
     2221 caagtatctt cttgaccagg gacagaccgg actaagtgtt gcctttgacc taccaacaca
     2281 gataggctac gattcagatc atggactgtc tgaaggagag gttggaaaag taggggttgc
     2341 aattgactca ctaagggata tggaaatttt gtttgatgga attccattgg acaaggtaag
     2401 tacgtctatg acaataaatg ctcctgcaag cgttttactt gcaatgtaca ttgcagtggc
     2461 agaaaagcag ggagtatctc cagacaaact aagaggtacc attcaaaatg acatactaaa
     2521 agagtatatt gcaagaggaa catatatatt cccagttgag gaatcaatga ggctgataac
     2581 aaacatcttt gagtactgct caaaggaggt tccacaatgg aataccataa gcatcagtgg
     2641 ataccacatt agagaggctg gttcaacagc ggctcaggag gtggcattca cattggcaga
     2701 tggaattgca tatgttgacg cagccatcaa ggcaggactt gatgttgacg atttcgcacc
     2761 tagactttcc ttcttcttca atgcccacaa tgaccttctt gaggaggttt ccaagtatag
     2821 ggccgcaaga agactatggg caaggataat gaaggaaaga tttggagcaa agaaggaaaa
     2881 gtcacttcag cttaagttcc acacacagac tgcaggatca acattgactg cccagcagcc
     2941 tgacaacaac attgtaaggg ttgcaatcca gaccttggca gctgttcttg gaggaacaca
     3001 gtcactacac accaactcaa gggacgaggc actggctctt ccaacagagg actcagtaag
     3061 aatcgccttg agaacacaac agattgtggc acacgagtct ggagtaacgg agacagtgga
     3121 tccacttgca ggatcatatt acatcgagag ccttacaaac gacattgaaa aggaagcaat
     3181 ggattacatt gaaaagatag acaatcttgg tggagcacca aaggccattg agaaaggatt
     3241 tatccagaag gagattcaga actctgccta caagtaccag atgcaggttg aagaccagga
     3301 aagaattgta gttggggtaa acaagttcag aattgatgag actgaaaaga aagagcttct
     3361 aaaggtagac ccacatgttg agcttctgca gaagaagaaa atcgagaagc ttaagagcac
     3421 aagagacaac gataaggtgc agggagcact tgagctattg aaagcaaagg cagaaacaga
     3481 tgaaaacctt atgccgttca taatcgaagc agtcaaggaa tacgcaacat tgggagaagt
     3541 ctgtggagtt ttgagagaag ttttcggaga atatgaacaa tctgtaatac tatagttatg
     3601 aggaggaaat aaaatggata aacccattag agtacttgtt gcaaagccag gtttggacgg
     3661 acacgacagg ggtgcgaagg ttatagccag agctttgaga gatgcaggaa tggaagtaat
     3721 ttatacagga cttagacaga caccggaaca aattgtggct gctgcagtcc aggaggatgt
     3781 tgacgtagtt gcaatgagca tccttt
//
LOCUS       DASRQF010000119         4614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1639408, whole genome shotgun sequence.
ACCESSION   DASRQF010000119 DASRQF010000000
VERSION     DASRQF010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639408"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1666)
                     /locus_tag="VFD79_03085"
     CDS             complement(<1..1666)
                     /locus_tag="VFD79_03085"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZH93062.1"
                     /translation="MEIQGIVEDVIYRNEANGYTVGRLYTSDGSITFVGQVPYIQTDQ
                     PVLLKGEWIYHNKFGEQFQFQSMETIMPTTTKGIENYLGSGLIPHIGPKTATRIVERF
                     GEKSLDIIQYYPERLLEIEGIGKKKLRVIMESFDEQREIREVMVALQEFNISPNQSMK
                     IYREYGNETLKVIRENPYRMAEDIHGMGFRTADEIAEKVGLEKSSPHRLQAGLKYVLT
                     RAAGEGHCYLPHGDLMDKAAGLLQIGKEGLDDSLTALILGKSFHLVENGEERIVYYMP
                     YHMAENSVAKVLVELSLVEQDEIVGDVEKALDNLEKGQDFTFGRRQREAVQSVLSHGV
                     TVITGGPGTGKTTIINAIIALMEAEGKEILLAAPTGRAAKRITETTGREARTIHRLLE
                     ISYQDESLQFNKNEEEPLDADVVIVDEMSMVDILLMNQLLKAIKPGTRLILVGDVDQL
                     PSVGAGNVLRDIIDSGVIKVVRLDEIFRQGEESMIIVNAHLVNRGEKPLLNEKDKDFF
                     FVYKDTGPEVRETIVQLCLQRLPDFYKLDPIKDIQVLSPMRKGDTGV"
     gene            complement(1751..2932)
                     /gene="metK"
                     /locus_tag="VFD79_03090"
     CDS             complement(1751..2932)
                     /gene="metK"
                     /locus_tag="VFD79_03090"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005957962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HZH93063.1"
                     /translation="MKKWLFTSESVTEGHPDKVCDQISDAILDAILEQDPEARVACET
                     MVTTGMVLVSGEITTTCYADIPKIVRKTIADIGYVRAKYGFDADTCAVLTSINEQSPD
                     IALGVDKALEQKEMSEDELDKIGAGDQGMMFGFACNETDELMPLPISLAHKLSRKLAE
                     VRKDDTLNYLRPDGKTQVTVEYHDDKPVRIENVVVSTQHSPVVSLEQIREDIEKYVID
                     EIIPADMMDENTKIYVNPTGRFVVGGPMGDAGLTGRKIIVDTYGGYARHGGGAFSGKD
                     ATKVDRSAAYASRYVAKNIVAAGLADKCEVGLAYAIGVAHPLSIFVDTFGTGKISDEE
                     ITELVKKHFDLRPAAIIRDLDLRKPIYRQIAAYGHMGRTDVELNWEKTDKVEILREAL
                     K"
     regulatory      complement(2991..3088)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            3308..3384
                     /locus_tag="VFD79_03095"
     tRNA            3308..3384
                     /locus_tag="VFD79_03095"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3342..3344,aa:Pro,seq:tgg)
     gene            3667..4167
                     /locus_tag="VFD79_03100"
     CDS             3667..4167
                     /locus_tag="VFD79_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZH93064.1"
                     /translation="MSKYLILYRHGIAEDKESAEEVDLRSLSTRGKALLKKSVSGFKT
                     MISNYGDLKIYSSPTLRAKETAEMLAEELGLDEPTYLEFLGTGSGVSRLRELLNEIEP
                     GDAAIVVGQQPYLSLWSQEISGTFLRFKKGSAACFKFPDEEVGESKADLRWFLQTRGF
                     VKIDDK"
     gene            complement(4277..>4614)
                     /locus_tag="VFD79_03105"
     CDS             complement(4277..>4614)
                     /locus_tag="VFD79_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93065.1"
                     /translation="GNEFQGTRVEVRWLFLADQRPYNPPDDPDDPDRPDRQDRPDNPE
                     DPEEILEIREEIPQAVPEPEQAEIIEAEEEIPLMIPILPRTGVVPAGLYYILGSLLFA
                     VGVRMKKKD"
BASE COUNT         1120 a         1142 c          935 g         1417 t
ORIGIN      
        1 acactccggt atctcccttt ctcattgggg ataggacctg tatgtccttt attgggtcca
       61 acttatagaa atccggcagt ctctgaaggc atagctgtac aattgtttcc ctcacttcag
      121 gaccggtatc cttgtataca aagaaaaagt ccttgtcctt ttcgtttaga agaggctttt
      181 cacctctgtt gaccaggtgg gcattaacaa ttatcatgct ctcctctccc tgacggaaga
      241 tttcatcgag cctcaccacc tttattacac ctgagtctat aatatccctg aggacattac
      301 cggctcccac agagggcagc tggtccacat ctcccacaag gataagcctt gtccccggct
      361 ttatggcctt aagaagctgg ttcatcagga ggatgtcaac cattgacatc tcatccacta
      421 ttacgacatc agcatccagg ggctcctcct cattcttgtt gaactgaagg ctttcatcct
      481 ggtaggagat ctcaagtagc ctgtggattg tcctggcctc ccgtccggtg gtctctgtta
      541 ttctctttgc agcccttcca gttggtgcgg ccaggaggat ttccttgccc tccgcctcca
      601 tcagtgcaat tattgcattt attattgtgg tcttacctgt tcctgggcca ccggttatta
      661 cagtaacccc atggcttaga acactctgga cagcttccct ttgcctcctt ccaaaggtga
      721 aatcctgacc cttttcaagg ttatccaggg ccttctccac atcccccaca atctcatcct
      781 gttcaaccaa gctcagttct acaaggacct tggcaacgct gttttccgcc atatggtagg
      841 gcatgtagta aacaatcctt tcctccccat tctcaaccaa gtgaaagctt ttaccaagga
      901 tcaaggcagt cagggaatca tccagacctt cctttccaat ttgaaggagt cctgcagcct
      961 tgtccataag atccccatgg ggcaggtagc agtgtccctc tccggcagcc cttgtcagta
     1021 catacttgag tccagcctgg agcctgtggg gtgatgactt ctccaatcct accttttcag
     1081 caatctcatc cgccgtccta aagcccatcc catgtatgtc ctcagccatc ctgtaggggt
     1141 tctccctgat taccttaagg gtttcatttc catactccct gtagatcttc atgctttggt
     1201 tggggcttat attgaattcc tgaagtgcca ccattacctc ccgaatctcc ctttgctcgt
     1261 caaaggattc catgatgacc ctgagctttt tctttcctat tccttcaatc tccaggagcc
     1321 tttcaggata gtactggatt atatcaaggg atttctcacc aaacctttcc actattctcg
     1381 tggcggtttt tggcccaatg tgtggaataa gtcctgaacc caagtagttc tcaatgccct
     1441 tggttgttgt gggcataatt gtctccatgg actggaactg gaactgttcc ccaaatttgt
     1501 tgtggtatat ccattctccc tttagaagca ctggctggtc agtctggatg tagggaacct
     1561 gacccacaaa ggttatgctc ccatctgacg tgtaaagtct tccaactgtg tagccattgg
     1621 cttcattcct gtagattaca tcctcaacta ttccctgaat ctccacattc tcaccccatc
     1681 tctcttcatt atttccatta taacaagaaa tcccgtcaat atacaataaa gtatattgac
     1741 gggatctgga ttatttcaac gcttccctta gtatttcaac cttgtccgtc ttctcccagt
     1801 tcagttcaac atcggttctg cccatgtgac cgtaagcggc aatctgtctg tagataggct
     1861 ttctaaggtc aaggtctctg atgattgcag ctggtctaag gtcaaagtgc ttcttgacca
     1921 actcagtgat ttcctcatca cttatcttgc ctgttccgaa ggtatcaaca aatattgaaa
     1981 gtggatgtgc aactcctatt gcataggcaa gtccaacctc gcacttgtca gcaagtccgg
     2041 ctgcaacaat gttctttgca acatatcttg atgcataggc tgctgacctg tcaacctttg
     2101 tggcatcctt gccggagaat gctccccctc cgtgccttgc gtatcctccg taggtatcaa
     2161 caatgatctt tcttcccgtt aggcctgcat cacccattgg tccgcccaca acaaaccttc
     2221 ctgttgggtt cacatatatc ttggtgttct catccatcat atctgcaggg atgatttcat
     2281 cgataacata cttctcgata tcctccctga tctgctccaa tgaaaccaca gggctgtgct
     2341 gggttgacac tacaacgttc tcaatcctta cgggcttgtc gtcgtggtat tcaacagtaa
     2401 cctgtgtctt gccgtctggc ctaaggtagt tgagagtgtc gtcctttctt acctccgcca
     2461 gctttcttga cagcttgtgg gcaagtgaga ttggaagtgg catcaattca tcagtctcgt
     2521 tgcaggcaaa tccaaacatc atgccctggt caccagcacc aatcttatct agctcatctt
     2581 cgctcatttc cttctgctca agtgccttgt ccactcctag ggcaatgtca ggtgactgct
     2641 cgtttatgga tgtcagtacc gcacaggtgt cagcatcaaa gccatatttc gcccttacat
     2701 agcctatgtc agctattgtc tttctaacta tctttggaat gtctgcataa caggttgtag
     2761 tgatttcacc tgatacaagc accattcctg ttgttaccat tgtttcgcaa gctaccctgg
     2821 cttcagggtc ctgctcaagg attgcatcaa gtattgcatc tgatatctgg tcgcagacct
     2881 tatcaggatg tccctctgtc acggactctg atgtaaataa ccattttttc atcttcttcc
     2941 tccttaaaat taaaaaacct tgtcgtaaag acaaggcgag tttgcactac acctcatctt
     3001 tcagaatatc attctgtagg atttagcacc tgccttcaca ggttgccggg tttcataggg
     3061 cctatccctc caccactctt gataaggtaa tcaagatatt aatattcact taaatgaata
     3121 atagcacaag agggtatggt agtcaaatat tttttgcgca attttttccc tcaaaccatg
     3181 ttgactttgc accttgtcct ctatataatg ttatatacag cttctgaaga gtgacaaaat
     3241 aattttcgaa aaagctcttg cattttaatc tgttttgcct tataataaag aagttgacaa
     3301 aaaacgtcgg ggtgtagcgc agtttggtag cgcacgtggt ttgggaccat ggggccgggg
     3361 gttcaaatcc tctcaccccg accaagagag aacgcagtga tcttaatgat tgctgcgttt
     3421 ttattttttt gtgttttggt gggatttgcc aaaaaaagtg cccatggggt cgtacccctt
     3481 ggctactttt cttcccccat tcactttcct ttgtatagat gttgttaatt tggtataatg
     3541 gactttaaga gtaccatcag gctattacaa agaaaaagta cataaatatt cttatcaagt
     3601 atttttttct tgtgaaattg ggtaatatgc ctaatgagga cattattcga aaagggggaa
     3661 tcaaatgtga gtaaatatct tatactgtat agacatggga ttgcggagga caaggagtct
     3721 gcagaagagg ttgatcttag gtcactgtcc acacgtggca aggctctatt gaagaaaagt
     3781 gtttcaggct ttaaaacaat gatcagtaac tatggagatt tgaaaatcta ttcaagccct
     3841 acccttagag caaaggaaac ggctgaaatg ttggctgaag agttgggatt ggatgagcca
     3901 acatatctgg aattccttgg cacaggaagt ggtgtcagta ggctcagaga gcttcttaat
     3961 gagattgaac ctggagacgc tgcaatagtg gttggacagc agccatacct ttctttgtgg
     4021 agccaggaaa tttctggaac tttcctgcgt ttcaaaaagg gaagtgcagc ctgcttcaaa
     4081 ttccctgatg aagaggttgg agaaagcaag gctgacctga ggtggttcct gcaaacccgt
     4141 ggctttgtaa agattgacga taaatagaaa tagaagaaac gatgagattg agacttttca
     4201 tcgtttcttt ttttgtgttt agaaattttt gtcaaaatgg gaccaaccaa aagcaaaaag
     4261 aactgtcccc aatgctttaa tcttttttct tcattcttac accaactgca aataacaagc
     4321 ttcccaggat atagtacaag cctgcaggaa cgactccagt tcttggtaga atcggaatca
     4381 taagaggaat ctcctcctct gcttcaatta tttcagcctg ctcaggctct ggaactgcct
     4441 gcggaatttc ttcccttatt tccagaatct cttcagggtc ttcaggatta tccggtctgt
     4501 cttgtctgtc tggcctatcc ggatcatctg gatcatcagg tgggttatat ggtctctggt
     4561 ccgctaaaaa caaccatcta acctccaccc tagttccctg aaactcattt cctg
//
LOCUS       DASRQF010000120         5188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1814338, whole genome shotgun sequence.
ACCESSION   DASRQF010000120 DASRQF010000000
VERSION     DASRQF010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1814338"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..473)
                     /locus_tag="VFD79_03110"
     CDS             complement(114..473)
                     /locus_tag="VFD79_03110"
                     /inference="COORDINATES: protein motif:HMM:NF015729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HZH93066.1"
                     /translation="MTALKQLGLILLILWAGQTIQSIFSLSLPGNVLGMIILLLLLLT
                     GILPLNLVEKISDVLLTHLTFLFLPTVVGIITVAHLLGDNLIWLMLILGASLVVVMVT
                     TGLTVQWLVRKREGKED"
     gene            complement(530..1021)
                     /locus_tag="VFD79_03115"
     CDS             complement(530..1021)
                     /locus_tag="VFD79_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HZH93067.1"
                     /translation="MLKEELQVIKQAIMNEVEGFEFYRMAAAQAGEGGSKDAFLELAN
                     EEKKHIEYLEAMFIKIKDGMEDEKAMAFMEGPPSPEIYKWDKLDKDLTSLAMSVFSIG
                     IQMEKDSVDFYTKAKEETEIEEARKLYDTLIKWEKVHLEQFTEQYNMYKEDWWSDQGF
                     APF"
     gene            complement(1091..2440)
                     /locus_tag="VFD79_03120"
     CDS             complement(1091..2440)
                     /locus_tag="VFD79_03120"
                     /inference="COORDINATES: protein motif:HMM:NF016488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(p)ppGpp synthetase"
                     /protein_id="HZH93068.1"
                     /translation="MKLELFDFIDQTLFLIEDKSDELNRVAGELEKFFNDSLFIKDHF
                     LNVNYRIKSSESLKEKILRHNFYLKYETPQMLVEDLSDLIGFRIECRFIEDEAKIYED
                     IVKLFNRREEGGYYSNPLNGSISLLLDEKQPQIQKNGFEIYKIDGKYSKDGVVVNFEL
                     QIKSMVNVFWGDIDHRVLYKNFNYMLTEDFFRDIMSSIKDNLTMIDRQLMLIYNHLSS
                     MDASSTPAKRAQMKGLLSKIIHDIYIVKVRNEVGFVLDFKKSTDVIVNFVFMRVEDGN
                     GSYSENFLRVMNRLNDIGRSDIDFNNYIEFEREIGYNDQFSRKFGNRILQVINKDFRW
                     NLFFKIISEIEEGTNTEDFEGFMHYLRYRYREVVQRSMEGMKLQDSERSDIEDCLLMY
                     IYESFHPEASIDFINNCTIATLGDKLLHLIGPLNSYEDWLRNREPIREAIVEFGKVE"
     gene            complement(2545..3642)
                     /locus_tag="VFD79_03125"
     CDS             complement(2545..3642)
                     /locus_tag="VFD79_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002834993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HZH93069.1"
                     /translation="MSSIVISTVSISAITALFAVLLTIADRTIGNYGQVTLTINDDKQ
                     FTVDGGSSLLSTLIEEKIFIPSACGGKGTCGYCKVQILDGGGPVLATETPFMTEEELA
                     NNVRLSCQCKVKQDIRIQIPEELFNVKEFEVTVEAIEDLTDVIKKLRFKLPEGEEINF
                     KPGQYVQLKAPLYEGNDEEVYRAYSIASSPLEKGYIELVIGFVPEGIATTYVHHHLEV
                     GDKATINGPFGDFYYQDNDREMVMVAVGTGVAPIMSLLNFMKSKGINRKARFYFGAKT
                     PSDLFMLDYFKELEETLPDFKFIPCLSRVGEEDQWDGFKGRVNNAIEKMMEEGEGKEA
                     YLCGNEPMIDSVVKSLTAKGMPEELIYYDKF"
     gene            complement(3655..4245)
                     /locus_tag="VFD79_03130"
     CDS             complement(3655..4245)
                     /locus_tag="VFD79_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002834994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HZH93070.1"
                     /translation="MIELNPFVIFIAAIFTNNMILSNFLGMCSFVAVSKEINTSLGLG
                     QAVTFVLAGTSIINYLVYHYLLVPYNLEFLRFIIFIISIAAFVQLIEMVLERYIPNLY
                     YALGIFLPLITVNCAILGVSLFMVIRDYTFLQSFGFAVGSGLGWTLAIVALAGIRQKL
                     KRASVPKGLEGPGITLIVTGLMALAFIGFSGIVQIS"
     gene            complement(4242..4862)
                     /locus_tag="VFD79_03135"
     CDS             complement(4242..4862)
                     /locus_tag="VFD79_03135"
                     /inference="COORDINATES: protein motif:HMM:NF006777.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HZH93071.1"
                     /translation="MAKQKSKEIFSMGVWLDNPILRQLIGICSALAVTNLMVNSLVMG
                     VGLTFVTAFSSLTISAIRHYTPKHIRMMVQVLIIASYVIMVDIFLKAYMPEMSKALGP
                     YIGLIITNCIIMGRAEAYAQKNPPLPSFLDGVANGIGYTGVLLFIAFFRELLGFGSLF
                     GIQILGDWWTAWTIMIMPPSAFFLLAIAIWIAHNLREKHQSEGAKS"
     gene            complement(4866..>5188)
                     /locus_tag="VFD79_03140"
     CDS             complement(4866..>5188)
                     /locus_tag="VFD79_03140"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HZH93072.1"
                     /translation="PVAGAGLWGSIVGYVGISEDYNTILGLTFTQQSETPGLGGRIEE
                     EEFLSQFRGLDISGSAEEEYVVYNPAPGGNVDAIAGATLTSKSVANFMNDDIHVFLQE
                     RRGE"
BASE COUNT         1341 a         1275 c         1024 g         1548 t
ORIGIN      
        1 ggggatttag tagcatgatc ttggttctcc ttgcaatgta gcttgaaatg gtgaatgcca
       61 gtatggatac tccgactccg aatattggcg tatttatata atccatcatt ccatcagtcc
      121 tcctttcctt ctcttttcct cacaagccac tggaccgtaa gtccagttgt caccatgaca
      181 accacaagac ttgctccaag gataagcata agccaaatca gattgtcccc taagagatgg
      241 gcaactgtaa ttatcccaac taccgttggc aaaaatagaa aggtcagatg tgtcagcagc
      301 acatctgata ttttttcaac cagattaagg ggcaatattc ctgtcaggag gagaaggagc
      361 agaataatca ttcctagcac attcccagga agggataggc taaaaatgct ttgaatcgtc
      421 tgtcctgccc acagtatcag caatatgagt cccaattgtt tcaatgccgt catttaccct
      481 cattcccttc tacaaagaaa taacagggta gcccctgtca tttcaagtct taaaacggtg
      541 caaagccctg gtctgaccac cagtcttcct tgtacatgtt gtactgctcg gtaaattgct
      601 caagatgtac tttttcccat ttgatcaatg tatcgtaaag ctttctcgcc tcttctattt
      661 cagtttcttc ctttgccttt gtatagaagt ccactgaatc cttttccatt tgaattccaa
      721 tgctgaaaac cgacattgcc agggaagtca aatccttgtc cagtttatcc cacttgtata
      781 tttctggtga cggaggccct tccataaatg ccattgcctt ttcgtcttcc attccatcct
      841 tgatttttat gaacatggct tcaaggtatt ctatatgctt tttctcctca tttgccagct
      901 caaggaatgc atccttgctt ccaccctcac ctgcctgggc ggcagccatc ctgtaaaatt
      961 caaaaccctc tacctcattc atgatagcct gcttgattac ttgcagttct tcctttaaca
     1021 tatgatcacc ctttctattt gattgctttt acatacatac ccaattagta aagaaatatc
     1081 actgtttttc ctattcaacc ttcccaaatt ccacaattgc ttcccttatg ggttccctgt
     1141 tcctcagcca gtcctcatag ctgttcaagg ggcctataag atgtaaaagc ttgtccccaa
     1201 gggttgctat tgtgcagttg tttataaagt caatgctggc ttctggatgg aaactctcat
     1261 agatgtacat aaggaggcaa tcctctatgt cgcttctttc tgaatcctga agcttcatcc
     1321 cctccattga cctttgaaca acctcccggt agcggtatct caggtagtgc ataaagcctt
     1381 cgaaatcctc agtgttagtt ccctcttcta tctcggaaat tatcttgaag aagaggttcc
     1441 accggaagtc cttgtttatg acctgtagga tccggttgcc aaatttcctt gaaaactggt
     1501 cgttgtatcc aatctccctt tcgaactcta tatagttgtt aaagtcaatg tcgcttctcc
     1561 cgatatcatt caacctgttc atgaccctaa ggaagttttc gctatagctg ccattcccat
     1621 cctcaaccct catgaagaca aagtttacga ttacatctgt ggacttcttg aaatcaagaa
     1681 caaatcccac ctcgtttcga accttgacta tgtatatgtc gtgaatgatc ttggatagca
     1741 gacccttcat ctgggctctt ttggccggtg tagagctggc atccatactg gaaagatggt
     1801 tgtatataag cataagctgc ctgtctatca tggtcaggtt gtcctttatt gatgacatta
     1861 tgtccctgaa gaagtcctcg gtcaacatgt agttgaagtt cttgtagagg accctgtggt
     1921 ctatgtcccc ccagaagaca tttaccattg acttgatctg gagctcaaag ttgacaacta
     1981 ccccatcctt ggagtacttg ccgtctatct tgtagatctc aaagccgttt ttctgtatct
     2041 gtggctgctt ttcatcaagt agaagtgata tgctcccgtt aagcgggttg gaatagtagc
     2101 caccttcctc ccttctgttg aagagcttta caatatcctc atatatcttg gcctcatcct
     2161 cgataaacct acattcaatc ctgaaaccga taaggtcaga taggtcctcc acaagcattt
     2221 gtggagtctc gtacttcaga tagaagttgt gtctaagtat cttctcctta agactttcgg
     2281 aggattttat cctgtagttc acattcagga agtggtcctt tataaagagg ctgtcgttga
     2341 aaaacttttc cagctcccca gcaaccctgt tcagttcatc agacttgtcc tcaatcagga
     2401 ataacgtttg gtcaataaaa tcaaatagct ctagtttcat cttaaccctc cccataggct
     2461 ctctcttcat tataccatat aggttgatga gattgaatca ataatagaca tgaaaagccc
     2521 ccggattctc cgggggttct taatctagaa tttgtcgtag tatataagct cttcaggcat
     2581 tcccttggct gtaaggctct ttacaacact gtcgatcata ggctcattgc cgcaaaggta
     2641 ggcctccttg ccttcgcctt cctccatcat cttttcaatt gcgttgttta cacgcccctt
     2701 aaagccatcc cactggtcct cttccccaac acgggacagg catggaatga acttaaagtc
     2761 aggcagggtc tcctccaact ccttgaagta gtcaagcata aacaggtctg aaggagtctt
     2821 ggctccaaag tagaacctgg cctttctgtt tattcccttt gatttcatga agtttagaag
     2881 tgacattatt ggagcaactc cagtaccaac tgcaaccatt accatttccc tgtcgttgtc
     2941 ctggtagtag aagtctccga atggaccgtt aatggttgcc ttgtcaccca cctcaagatg
     3001 gtggtggaca taggttgttg ctataccctc aggtacaaaa ccaattacaa gctcaatgta
     3061 gcccttctcc agtggagatg aggcgattga gtaggccctg tagacctctt catcgtttcc
     3121 ttcgtaaagg ggagccttaa gctgtacata ctggcctggc ttgaagttga tctcctctcc
     3181 ctcaggcagc ttgaacctaa gcttcttgat tacatcagtc aaatcctcta tggcttcaac
     3241 ggtgacttca aactccttga cgttgaacag ttcctctgga atctgaatcc tgatgtcctg
     3301 cttaaccttg cactggcagg atagccttac attgtttgca agctcctcct cggtcataaa
     3361 tggggtttcc gttgccagaa caggtccccc accatcaagt atctgaacct tgcagtagcc
     3421 gcaggttccc tttcccccac atgctgaggg tatgaatatt ttttcctcta tcaaggtgga
     3481 caggagggaa gaacctccgt ccactgtaaa ctgcttgtcg tcgtttattg tcaaggtcac
     3541 ctgaccatag tttcctattg tcctgtctgc tatggtaagc aggacggcaa acaatgctgt
     3601 tattgccgaa atggatacgg ttgagattac tattgaactc atttaatccc ccccttaaga
     3661 tatttgcacg attccggaga aaccaataaa tgccaatgcc atcaagcctg taacaatcag
     3721 ggtgatgcca gggccttcaa gtccctttgg aacgcttgct ctcttgagct tctgtctgat
     3781 tcctgccagg gcaacaattg ccagggtcca accaagacct gacccaactg caaagccaaa
     3841 tgactgcagg aatgtgtagt cacggattac cataaagagg gatacaccaa gaatggcaca
     3901 gtttacggtt attagtggaa ggaatatacc tagtgcatag taaagattag gtatgtatct
     3961 ctccagtacc atctcaatca gctgtacaaa ggcagctatg gatattatga atatgatgaa
     4021 cctcaaaaac tccaggttgt agggtaccaa aaggtaatgg tataccaggt agttgataat
     4081 tgaagtacct gcaagtacaa aggttacagc ctgtcccaga cccagtgaag tgttgatctc
     4141 cttggaaaca gcaacaaagg agcacattcc caggaagttg gacagaatca tattgttggt
     4201 gaatattgcc gctatgaata ttacaaatgg attcagttca atcatgattt agccccctca
     4261 ctttgatgct tttcccttaa gttatgtgca atccagattg ctatggccaa caggaagaac
     4321 gcacttggcg gcattatcat gattgtccat gcagtccacc agtctcccag aatctggatt
     4381 ccaaaaaggg atccaaatcc aagcagctct ctaaaaaatg ctataaatag aagaactcct
     4441 gtgtatccga tgccatttgc aaccccgtca aggaatgatg gcaggggtgg attcttctgg
     4501 gcataggctt cagcacgacc cattattata cagtttgtta tgatcaaacc tatgtagggt
     4561 cccagagcct tgctcatttc aggcatgtat gccttaagga atatgtctac cattattaca
     4621 tatgacgcta tgattagtac ctgaaccatc atcctgatat gctttggtgt gtaatgtctg
     4681 attgcggata tggtcagtga tgaaaatgcc gttacaaatg taagtcctac acccattaca
     4741 agggaattga ccataaggtt ggttacagca agggctgaac atattcctat aagctgcctc
     4801 agtatggggt tgtcaagcca gacacccatt gaaaaaatct cttttgactt ctgcttggcc
     4861 atgacctatt ccccccttct ttcctgcaag aagacatgaa tgtcgtcatt catgaagttt
     4921 gcaactgatt tggatgtcaa ggtagctcca gcaatggcat ccacgtttcc tcctggagcc
     4981 ggattgtata ccacgtactc ctcctctgca gaccctgata tgtcaaggcc tctgaactgg
     5041 ctgaggaatt cctcctcctc gatccttcca ccaagtccag gtgtttctga ctgctgggtg
     5101 aatgttagac ccagtattgt attgtagtcc tctgaaattc ccacatatcc cactatgctg
     5161 ccccacaggc cagctccggc aacaggga
//
LOCUS       DASRQF010000121         2141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_759131, whole genome shotgun sequence.
ACCESSION   DASRQF010000121 DASRQF010000000
VERSION     DASRQF010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759131"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="VFD79_03145"
     CDS             <1..1160
                     /locus_tag="VFD79_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HZH93073.1"
                     /translation="GWIDEYFDDLGELFNATHKAMEEKKATAIAYHGNIIDLLEYALE
                     KDIHIELLSDQTSCHAAYDGGYCPQGLTFEERTKMLLEDKKSFIKEVDKSLKHHFSLI
                     QKLVDKGTYFFDYGNSFMKAIYDAGVMEISANGIDDTDGFIFPSYVEDIMGPMLFDYG
                     YGPFRWVCLSGKHEDLIRTDKAAMDCIDPDRRGQDRDNYNWIRDAEKNKLVVGTQARI
                     LYQDEVGRTQIALKFNEMVRNGEVGPIMLGRDHHDVSGTDSPFRETANIKDGSNIMAD
                     MATHCFAGNAARGMSLIALHNGGGVGIGKSINGGFGMVLDGSERVDTILSLAMPWDVI
                     SGVARRAWARNDNSLDTIIEFNKNNKETDHITLPYVADDKLVKDLVQKNYK"
     gene            1213..>2141
                     /locus_tag="VFD79_03150"
     CDS             1213..>2141
                     /locus_tag="VFD79_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HZH93074.1"
                     /translation="MEKHYDGTKIAKFIGFALVGIFMFFIPITINEKSTIPLDHIVSY
                     IQTIPGWGPVYSGLIVTIGGLLPFIRKDWKKSTSDMVFSIFKLAGIVLIAMAIFGFGP
                     AFLHAPDIIPFIYGKIVVPVTTIVPVGAIFLAFLVNYGLMEFIGVFMQPVMNPIWKTP
                     GRSAIDAVASFVGSYSLGLLITNQVYTEGKYTGKQAAIIATGFSTVSATFMVIVAKTL
                     GLMEYWLLYFWLTLVITFIVTAITSRIYPLNKKPDTYFNNMEGSPEPVPKGNRLRRAY
                     EEGMDAMEKAPTVAESVWDTLKKGIMLALSIGP"
BASE COUNT          649 a          438 c          524 g          530 t
ORIGIN      
        1 agggctggat agacgagtac tttgatgacc ttggagagct tttcaatgca acccacaagg
       61 caatggagga gaagaaggca actgccatcg catatcatgg aaacataata gaccttctcg
      121 aatacgctct ggaaaaggat atacacattg agcttctttc agaccagaca tcatgccacg
      181 cagcctatga tggaggctat tgtccacagg gactgacctt tgaggaaaga acaaagatgc
      241 ttcttgagga caagaagagc tttatcaagg aggttgacaa gagccttaag caccacttca
      301 gcctgataca gaagcttgtt gacaagggaa cctacttctt tgactatgga aacagcttta
      361 tgaaggcaat ctacgatgca ggtgtcatgg aaatttcagc aaacggaata gacgatacag
      421 atggattcat cttcccatcc tacgttgagg acatcatggg gccaatgctg ttcgattacg
      481 gctatggacc atttagatgg gtatgcctaa gcggcaagca cgaggacctt ataaggactg
      541 acaaggctgc aatggactgc atagatcctg acagaagggg acaggacaga gacaactaca
      601 actggatcag ggatgctgaa aagaacaagc tagtggttgg aacccaggcg agaatcctat
      661 accaggatga ggttggaaga acccagattg ccctgaagtt caacgaaatg gtcaggaatg
      721 gagaggttgg accaattatg cttggtaggg accaccatga cgtatcagga accgactcac
      781 cattcaggga gactgcaaac atcaaggatg gaagtaacat catggccgac atggcaaccc
      841 attgcttcgc aggaaatgct gcaaggggaa tgagccttat cgcccttcac aacggtggag
      901 gagtaggaat cggtaaatcc atcaatggcg gctttggaat ggttctggat ggatctgaaa
      961 gggttgacac catccttagc ctggcaatgc catgggatgt tatcagtgga gttgcaagaa
     1021 gagcatgggc tagaaatgac aactcactgg atacaataat cgagttcaac aagaacaaca
     1081 aggaaactga ccatataacc ctgccatatg ttgcagatga caagctggtc aaggacctgg
     1141 ttcaaaagaa ctacaagtaa atatgaggga gccagatcct ggctcccaaa attaaaatag
     1201 gagcgtgata aagtggagaa gcattatgac ggaacaaaga ttgcaaaatt tataggtttt
     1261 gccctggtgg ggatattcat gtttttcatt cccattacaa tcaatgaaaa gagcacaatc
     1321 ccccttgacc acatcgtaag ctatatccag accattcccg gctggggacc tgtttattca
     1381 ggattgatcg ttaccattgg gggactgctt ccctttatca gaaaggattg gaagaagagc
     1441 acatccgata tggtattttc aatcttcaaa cttgctggaa tagtacttat tgccatggca
     1501 atttttggat ttggtccagc ctttttacat gcacctgata taattccatt tatttacggc
     1561 aagatagtgg tgcctgttac aacaatcgtt cctgtgggag ccattttcct ggcttttctt
     1621 gtaaactacg gattgatgga gtttatagga gtcttcatgc agcctgtaat gaaccccata
     1681 tggaagacac cgggtagatc ggcaatagat gcggttgcat cctttgttgg tagctactca
     1741 cttggcctgc ttattaccaa tcaggtctat actgaaggaa aatataccgg gaagcaggct
     1801 gcaataattg caactggatt ctccacggtt tcagcaacct ttatggttat tgttgccaag
     1861 acacttggtc ttatggagta ttggctattg tacttctggc ttaccctggt aataaccttt
     1921 atagtaactg caatcacatc tagaatctat cctttgaata aaaagcctga cacgtatttc
     1981 aacaatatgg agggtagtcc ggagccggtt ccaaaaggta acagacttag aagggcatat
     2041 gaagagggta tggatgcaat ggaaaaggcc ccaactgtcg cagaaagcgt gtgggacacc
     2101 ttgaaaaaag gcatcatgct tgccctatca attggacctt t
//
LOCUS       DASRQF010000122         5141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_260959, whole genome shotgun sequence.
ACCESSION   DASRQF010000122 DASRQF010000000
VERSION     DASRQF010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260959"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VFD79_03155"
     CDS             <1..313
                     /locus_tag="VFD79_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HZH93075.1"
                     /translation="VLVLVAAVALVGCASGNGSEAPAEEGDRVFTLEELAEYDGLEGR
                     EAYVAVDGVVYDVTGSDMWDEGGHNGFQAGQDLTEGIKEASPHGVSVLSRMPVVGTLE
                     E"
     gene            332..724
                     /locus_tag="VFD79_03160"
     CDS             332..724
                     /locus_tag="VFD79_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93076.1"
                     /translation="MYSVLGWLNVIILGVILSPYVLGFLNRKFLKTRNKGFINTIKFL
                     RRLHKPFGLAMAVLALVHGYMAMGGFRLHTGSLLYASIFLTALLGGSFYKLKKRALFT
                     WHKRMALVSFLLLLLHLFYPSALYYLLN"
     gene            complement(749..2137)
                     /locus_tag="VFD79_03165"
     CDS             complement(749..2137)
                     /locus_tag="VFD79_03165"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HZH93077.1"
                     /translation="MKRTMILLMAIIIACGYSSAQGISSRARNPLKLHIDSLVVDGHN
                     DTMLRTIDEGTWLPREDIRKGTSLHADLWKLRAGNISVPFFAAYSSPFYGQPERSLSR
                     TLAIFNALYWTERNNPDILEIVSTYDEIEKALLNRRIAAVPTIEGAYSITHGNWEELL
                     LQYRDLGVKAIGFTWNYSNELAEGLYRAYGDYNGTPSEGGLTHLGIRTLERMNQLGII
                     ADVSHLSEESFWDVMANSKSPVMASHSGAYGAYPHPRNLKDDQILAIGENGGVIGVVY
                     HDEFVAGPGKTYVKDIVDHMEHIIDLVGVDHVGLGSDFDGAYLPVDLEDASKVYRITG
                     ELVERGYGDREIRKILGLNMLRVIKENDQLAEIRPLLEGVVITPDFSMGEIFQNGRPV
                     FSADISGDHENLEIYGVVDGRRYPVQRDIGKLSMTPELDLREKFHLVTFVADHPTRGD
                     QRETRIVYIEQD"
     gene            complement(2155..3366)
                     /locus_tag="VFD79_03170"
     CDS             complement(2155..3366)
                     /locus_tag="VFD79_03170"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HZH93078.1"
                     /translation="MNNNDLVETNRGKQVVTAAALINFMTGMMYAWSIISKALVEDHG
                     WTSKEASLPYTIFTIAFPLAMVVFGKFQDEKGPRISGLLGSVLVGLGFILSGIFLDPF
                     AMLLTIGVMAGAGVGIITISTAPLAIKWFHPSMKGKITGIVVAGVGLSALLYSPMGTF
                     LVGSVGVARTLLYIGILVLPTTLFLALKLENPPEGYDFDGFKAKADYVYSRDIQWREM
                     LRTGEFYKLWIMLGLSSSAGLMVISHISSIANIQSGWKAGFILVMVISLFNSSGRIIG
                     GSLSDRIGRINTMRVLFLMQAANMLLFRNATSIPVMVVAAAIAGLCYGAGFSVFPAAV
                     GDYYGTKYFGFNYGLLFSGWGLGGIIGPMTGAAIYDATGAYGPAFLVSFGLLVVSFFI
                     TLTFRKKKAVE"
     gene            3548..4564
                     /locus_tag="VFD79_03175"
     CDS             3548..4564
                     /locus_tag="VFD79_03175"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HZH93079.1"
                     /translation="MKKGLKLTGLLLGILLVVSLFVGCGSMIAREESSMDSAAPYPAE
                     EPAAQAPAYEEAGEAPRENGMGFPSFSGSIEPDKIITTISMTFETTEFDQSLEKLYLL
                     IEEAGGYLSSSNVYYSSYYNSQMYQRGDFAIRIPRDKVNQFKGLVPQIGNLTNESTSK
                     EDVTRFYTDTESRLNVLEIKEERLLSLLERAEEIEDIIKLEDQLSEVIYEKENLKATL
                     VNLDDRIDYSTFNVALIEVAKVSTTETKETPFLTRIQNAFENSLYQFGRSAENFAVFM
                     VYNLPGLLVLLVVAIVLYKIIKRIVRGPGRPPGEKKPKKNNKDDRFVDKEDQRSEKKD
                     DSQA"
     gene            complement(4594..>5141)
                     /locus_tag="VFD79_03180"
     CDS             complement(4594..>5141)
                     /locus_tag="VFD79_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZH93080.1"
                     /translation="KWAEEAAKEAGAEVRLLKVQELIPQEIIDGNPRMKEIAEMTKDV
                     KIASSADLDWADAIIFSAGARFGNVPSQMKNFFDMQGGLWAEGKLANKVVSAMSSAQN
                     PNGGQEGAVKAMYSTFMHWGAIIAAPGYTDGSLFAAGGNPYGTTATVSQEGEIIDDVE
                     AAVKHQARRTVQIAEWIKKGK"
BASE COUNT         1338 a         1243 c         1153 g         1407 t
ORIGIN      
        1 ggttcttgta ctggtcgcag cagtggcact tgtaggttgc gcctcaggca acgggtcgga
       61 agcacctgca gaagaaggag acagggtctt caccctggag gagcttgcag aatatgacgg
      121 tcttgaaggt cgggaagcct atgtggctgt tgatggtgtt gtatatgatg taaccgggtc
      181 tgacatgtgg gatgaaggcg gacacaacgg ctttcaggca ggacaggatt tgactgaagg
      241 aataaaggaa gcttctcctc atggtgtttc tgttctcagc agaatgccag ttgtgggaac
      301 actggaagaa tagtgatttt gggggtgttg tatgtactca gtattgggat ggcttaatgt
      361 aataattttg ggagtgatcc tctcgccata tgtccttggg tttctaaaca ggaagtttct
      421 caaaacccgg aacaagggct ttataaacac aattaaattc ttgaggaggc ttcacaagcc
      481 ctttggtctt gcaatggctg ttcttgccct tgtacatggc tacatggcaa tgggaggctt
      541 cagacttcat acgggaagtc ttctttacgc ctccattttc ttgacggccc ttctaggggg
      601 aagcttctac aagctgaaga agagagccct ttttacatgg cacaagagga tggccctggt
      661 gtcgttcctg ctgcttttac tccacctttt ctatccaagt gcattgtact atcttctgaa
      721 ttgaaaaatc ccgggaaacc gggatttttt agtcttgttc aatatataca atccttgtct
      781 cccgctggtc tcccctggta ggatggtctg caacaaaggt tacaaggtgg aatttctccc
      841 taaggtcaag ctccggggtc atggaaagct ttccaatgtc cctttggacg ggatatcttc
      901 ttccatcgac tacaccataa atttccaagt tttcatggtc cccggaaata tctgcagaaa
      961 acactggcct tccattttga aatatctccc ccatactgaa gtcaggggtt attaccacac
     1021 cctcaagtaa tggtcttatt tcagccagct ggtcgttttc ctttattacc ctcaacatgt
     1081 taagtccaag aatcttcctt atctccctgt caccatagcc tctttccaca agctccccgg
     1141 ttatcctgta gacctttgag gcatcctcca gatccactgg caggtatgct ccatcaaagt
     1201 ctgaccccaa tcctacatgg tcaactccca caaggtctat tatatgctcc atgtggtcaa
     1261 ctatatcctt gacataagtc tttccaggtc ctgccacaaa ctcatcatga tagaccactc
     1321 ctatgactcc tccattctct ccaatggcta aaatctggtc atccttaaga ttccttggat
     1381 ggggataagc tccataggct ccggagtggg aagccattac aggacttttt gagttggcca
     1441 tcacatccca gaagctctcc tccgaaagat gactgacatc cgcgattatc ccaagctggt
     1501 tcatcctttc aagggtccgg ataccaagat gggtgagtcc accttcactg ggtgtgccat
     1561 tgtagtcacc gtaggccctg tagaggccct ctgcaagctc attggaataa ttccaggtaa
     1621 atcctattgc cttgactcca aggtccctgt attgcaggag gagttcttcc caattgccat
     1681 gggttatgga atatgctcct tctattgtgg ggacagctgc aatccttcta ttcaagagag
     1741 ccttttcaat ttcatcatag gttgagacta tctccaggat atccggattg ttcctttcag
     1801 tccagtacaa ggcgttgaaa atagccaggg ttctgcttag gctcctttct ggctgaccgt
     1861 agaagggcga tgagtatgct gcaaaaaaag ggacgctgat atttcctgcc cgaagcttcc
     1921 acaggtcggc atgaaggctt gtccccttcc gaatatcctc ccggggtagc caggtccctt
     1981 catctattgt cctgagcatg gtgtcgttgt ggccatccac aactagtgag tcgatatgaa
     2041 gcttaagtgg attccttgcc ctgcttgaga tcccttgggc agatgaatat ccacacgcaa
     2101 ttataattgc cataagaaga atcatcgttc tcttcatgag ctttctcctc ttttctactc
     2161 cacggctttc tttttcctga aggttagggt aatgaagaat gaaaccacca aaagaccgaa
     2221 gctaactaaa aaggctggtc catatgctcc tgttgcatcg tatattgctg cacctgtcat
     2281 agggccgatt attccgccca gtccccatcc tgagaagagg agaccatagt tgaatccgaa
     2341 atactttgtt ccatagtagt cgccaacggc agcagggaag accgagaagc cagctccgta
     2401 gcagaggccg gcgattgcag ctgcaacaac catgacaggt attgatgttg catttctgaa
     2461 aagcagcatg ttggccgcct gcataaggaa gagcaccctc atggtattga ttcttccaat
     2521 cctgtctgaa aggcttcctc caatgatcct acctgaactg ttaaatagcg atattaccat
     2581 tacaaggata aagcccgcct tccagccgct ttgaatattt gctatgctgg aaatgtggga
     2641 tattaccatc agtccagccg acgaagacag gcccagcatt atccagagct tgtagaactc
     2701 gcccgtccta agcatttctc tccattggat gtccctggag tatacatagt ctgcctttgc
     2761 cttgaatcca tcaaaatcat atccctccgg tggattctcc agcttaaggg caaggaagag
     2821 ggttgttgga agaaccaaaa tccctatata tagaagggtc cttgcaactc caaccgatcc
     2881 cacaagaaat gttcccattg gagagtagag caatgccgac agtccaaccc ctgcaacgac
     2941 aattccggtg attttcccct tcatgctggg gtgaaaccat ttaatggcca ggggagcggt
     3001 ggatatggtt atgattccaa cacctgcacc tgccatgaca cctatggtaa gaagcattgc
     3061 aaagggatcc aaaaatatac ctgacaggat gaaacccagg cccaccagga cacttcccag
     3121 aagccctgaa atacgtggtc ccttctcatc ctggaacttt ccgaaaacaa ccattgcaag
     3181 gggaaaagct atggtaaaaa ttgtgtatgg tagcgaagcc tccttgctgg tccagccgtg
     3241 gtcctcaacc aaggccttgc tgattatgct ccaggcatac atcatgcctg tcatgaagtt
     3301 tatcagggca gcagctgtta caacctgctt tcccctattt gtttctacaa ggtcattatt
     3361 gttcatacat gtctccttta atgtttgctg tatgtattat aacatgattt ggaggatttt
     3421 ttcacatatg gaccaaattt acatatcttg gtggcatttt gtagggcttt cttaacctga
     3481 ttgtaaccgg attgtatcac tttggatgtt tttctggggt ataattactc aaaaggagat
     3541 gagattaatg aagaaaggct taaaactaac aggtctgctt cttggaatcc tattggtagt
     3601 gtccttgttt gtgggttgtg gctccatgat agcaagggag gagtcttcaa tggacagtgc
     3661 tgccccctat ccagctgaag aacccgcagc gcaggctcct gcctatgaag aggccggaga
     3721 agctccacgt gaaaatggga tgggatttcc aagcttttca ggatccatcg agccggacaa
     3781 gatcataaca accattagca tgaccttcga gaccactgaa ttcgaccagt cactggaaaa
     3841 gctgtactta cttatagagg aggcaggggg ctatctttca agctcaaatg tatactactc
     3901 aagctattac aactcccaga tgtatcagcg tggagacttt gcaatccgga ttcccagaga
     3961 caaggtaaac cagtttaagg gattggttcc tcagataggg aaccttacaa acgaaagcac
     4021 ctccaaggag gatgtgacaa ggttctacac agatacggaa tcaagactga acgtcctgga
     4081 gataaaggag gaaaggctgc tttcattact tgaaagggca gaggaaatag aggacatcat
     4141 aaagcttgag gaccagctga gcgaagtcat atatgaaaag gaaaacctta aggccaccct
     4201 tgtaaacctt gacgacagaa ttgactattc tacattcaac gtagccctta tagaggttgc
     4261 aaaggtcagt accacagaaa ccaaggaaac acctttcctt acaaggattc agaatgcttt
     4321 tgaaaactcc ctctaccagt ttggccgatc agctgagaac tttgcggtat ttatggtcta
     4381 caaccttcca ggacttcttg tccttctggt agttgccata gtactttaca aaatcattaa
     4441 gaggatagtc agaggaccgg gaaggccccc tggagagaag aaacccaaga aaaacaataa
     4501 ggatgacaga tttgtggata aagaagacca aaggagcgaa aaaaaagatg attcccaggc
     4561 ttaatgcctg gaatcatctt tttcatttat tttctatttt ccctttttaa tccactctgc
     4621 aatctggaca gttcttcttg cctggtgctt gactgcggct tcaacatcat ctattatttc
     4681 accttcctgt gaaaccgtgg cagttgttcc gtatgggttg ccaccggcgg caaacagtga
     4741 tccatcagtg tagccaggag cagctattat agccccccag tgcatgaagg ttgagtacat
     4801 tgcctttaca gcaccctcct gtccaccatt tgggttctgt gccgaagaca ttgctgaaac
     4861 caccttgttt gcaagcttgc cttcagccca aaggcctccc tgcatgtcga agaagttctt
     4921 catctgtgaa ggaacatttc caaatcttgc tcctgcactg aatatgatcg catcagccca
     4981 atcaagatca gcggaggaag caattttaac atccttggtc atttccgcta tttccttcat
     5041 tctaggattc ccgtctatga tttcctgggg aataagctcc tgcaccttca agagcctgac
     5101 ctctgctcca gcttcctttg cagcctcttc agcccacttg g
//
LOCUS       DASRQF010000123         2825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_312805, whole genome shotgun sequence.
ACCESSION   DASRQF010000123 DASRQF010000000
VERSION     DASRQF010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312805"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /gene="lysA"
                     /locus_tag="VFD79_03185"
     CDS             <1..972
                     /gene="lysA"
                     /locus_tag="VFD79_03185"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HZH93081.1"
                     /translation="YEELLLAIEYGVGRIIVDGMEELQMIEEICRATQSKANVMYRIT
                     PGVKNETHEYITTGKKDSKFGIPLDQDVFYPAVEDAIRSPFVEFMGLHFHVGSQLFDN
                     ESHLNALDVLLDHVDEIRRRFDYEIREINLGGGFGIRYTDELRKPLSYFTDPLMDRIE
                     GHYQALGLERPAVVIEPGRSLVGEAGITLYTVGNIKDIEGVRKYVSVDGGMTDNIRPA
                     LYQADYTGVIANKVGEEMEDKVTICGKCCESGDILIRDIGIPNPESGDIFCVFSTGAY
                     GYSMASNYNMNPIPAVVFVENGKDTLVVRRQTYKDMLRNDIVPSWLE"
     gene            984..1823
                     /gene="dapF"
                     /locus_tag="VFD79_03190"
     CDS             984..1823
                     /gene="dapF"
                     /locus_tag="VFD79_03190"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HZH93082.1"
                     /translation="MEFTKMQGAGNDFIIINNIVEEIPEASLSDIAKALCSRRTSIGA
                     DGIMFIEEAEGCGDFRMHFFNADGSEGEMCGNGARCICRYAYENGLSGEKQKVETVSG
                     LVRGWRVDERTYRVRLNSPSDYREELWLELEDERFKCSYVELGSPGLPHLILNMPDFD
                     NLPEEDLYRIGKGLRWHESLPKGANVNFYYLGQDGSALARTFERGVEDFTLACGTGAG
                     SVGYVLVKTDQVSEDQVTIKMPGGELVIQVVLHPDGVEDIFLSGPATLVSDGRVLDED
                     LRF"
     gene            1853..>2825
                     /locus_tag="VFD79_03195"
     CDS             1853..>2825
                     /locus_tag="VFD79_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HZH93083.1"
                     /translation="MTNSNTNSIWKSYRFPIILLTGIIIGSIIGLVFGERAKVLAPLG
                     DLFINMMFTAVVPLVFLSISSAVGSMMNMKRLGRILGSMFAVFIVTGAIAAVLIIVVV
                     KAFPPAQGVNLVMEAATLDEISVGDALVNAFTVSDFPLLLSRSNMLPLIVFSIIFGFS
                     VSLVGGEDNKVAQALDMLNKVMMKMIGIIMLYAPIGLGAYFAALIAEYGPQLLGAYLR
                     SMAIYYPLCIAYFFIAFFAYSYWAGGKEGTRIFFKNILSPAVTSLATCSSIATLPVNL
                     TATEKMNVPKDIREIVLPIGATMHMDGTALSSILKIAFLFGIFGQDFS"
BASE COUNT          810 a          532 c          756 g          727 t
ORIGIN      
        1 tatgaggagc ttcttcttgc tatcgaatac ggagtgggaa gaattatagt ggacggcatg
       61 gaggagttac agatgataga ggaaatctgt agggctacac aaagcaaggc aaatgtaatg
      121 tacaggatca ctccaggtgt aaaaaacgaa acccatgaat acatcacaac ggggaagaag
      181 gattcaaagt ttgggatacc actggaccag gatgtgtttt atcctgcagt ggaggatgca
      241 atccggtcgc cttttgtgga atttatggga ctccacttcc atgttggatc acaactattt
      301 gacaatgaat cacatctgaa tgcattggat gttctgctag accatgttga tgaaatcaga
      361 aggaggtttg actacgagat tagggagatc aatcttggag gaggttttgg aatcaggtat
      421 acggacgaat tgagaaagcc actttcatat ttcaccgatc ccttgatgga taggattgaa
      481 ggccattacc aggccttggg tcttgagagg ccagctgttg tgatcgagcc aggcagaagc
      541 cttgtgggag aggctgggat aacactttac actgttggaa atataaagga catcgagggt
      601 gtcaggaagt acgtctccgt agatggggga atgacagaca atattaggcc ggctctttac
      661 caggcggatt atacaggggt cattgcaaac aaagtagggg aagagatgga agataaggtg
      721 accatatgcg gcaagtgctg tgagtcaggg gacattttaa ttcgggacat tggcattcca
      781 aacccagagt caggagacat cttctgcgta ttctcaactg gggcctatgg atattcaatg
      841 gcaagcaact ataatatgaa tcccatccct gccgttgtat ttgtagagaa tggcaaggat
      901 acattggttg ttaggagaca gacatacaag gatatgctga gaaacgatat tgtaccatct
      961 tggctggaat aagggggata ctgatggagt tcactaaaat gcaaggagca ggaaatgatt
     1021 ttataataat aaacaacatt gttgaggaga ttccggaggc atccttatca gatatagcaa
     1081 aggccctctg ttcaagaagg acttccattg gggccgacgg aataatgttc atcgaagagg
     1141 ctgaaggctg tggcgacttc agaatgcact tcttcaatgc cgatggcagt gagggagaga
     1201 tgtgcggcaa cggtgcaaga tgcatctgca ggtatgccta tgagaacggc ctttctggag
     1261 agaagcagaa ggttgagact gtatctggtc ttgtaagagg ctggagagtc gatgaacgca
     1321 cctacagggt aaggctgaac agccccagtg actacaggga ggagctttgg cttgagctgg
     1381 aggatgaaag gttcaaatgc tcatatgttg agcttggcag tcctggacta ccgcatctta
     1441 ttctgaatat gccagacttt gacaatcttc ctgaagaaga cctgtataga attggaaaag
     1501 gcttgagatg gcatgaaagt ctcccaaagg gtgcaaatgt aaacttctac tacctgggac
     1561 aggatggtag tgcacttgca aggacctttg agagaggggt tgaggatttt accctggcat
     1621 gcgggacagg cgcaggatcg gtgggatatg tacttgtgaa gacagaccag gtttctgaag
     1681 atcaggttac catcaagatg cctggaggag agcttgtaat ccaggtggtt cttcatcctg
     1741 acggagtgga ggacatcttc ctcagcggac ctgcaaccct tgtatcagat ggaagggttc
     1801 tggatgaaga ccttagattt taaacacaaa ttacatagac ggaggtttta acatgacaaa
     1861 ttcaaataca aactcaattt ggaaaagcta cagatttcct atcatactac ttacgggaat
     1921 tataatcggt tcaatcatag gcttggtgtt tggcgaaagg gcaaaggtgc tggcaccact
     1981 gggtgacctg ttcatcaata tgatgttcac ggcagttgta ccccttgtat tcctaagcat
     2041 cagcagtgca gtgggaagca tgatgaacat gaaaagactt gggagaatac ttggaagcat
     2101 gtttgcagtg ttcatagtga caggagcaat tgcagcggta cttatcatag ttgttgttaa
     2161 ggcatttcca cctgcccagg gtgtcaacct ggttatggag gctgcaaccc ttgatgagat
     2221 aagcgttggg gatgccttgg taaatgcatt tacagtaagt gactttccac ttctactgtc
     2281 aagatccaat atgcttccac taattgtttt ctcgattata tttggcttca gtgtaagcct
     2341 ggttggggga gaggacaaca aggtggcaca ggcattggat atgcttaaca aggttatgat
     2401 gaaaatgatt ggaatcataa tgctgtatgc accaataggt ctgggagcat attttgcagc
     2461 cctgatagca gagtacggac ctcagctgct gggggcctac ctgagatcaa tggcaatcta
     2521 ctatccactt tgcatagcct acttcttcat agcattcttt gcatattcat actgggcagg
     2581 aggaaaggag ggaacaagaa tattcttcaa gaacatcctt agtcctgcag tgacatccct
     2641 tgcaacttgc agctctattg caacccttcc tgttaacctg acggctacgg aaaaaatgaa
     2701 tgtgcccaag gacattaggg agattgtact tccaataggt gcgacgatgc acatggacgg
     2761 aacagccctt tcatcaatac tcaagatagc cttcctcttt ggtatattcg gccaggactt
     2821 ttcag
//
LOCUS       DASRQF010000124        10853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1502999, whole genome shotgun sequence.
ACCESSION   DASRQF010000124 DASRQF010000000
VERSION     DASRQF010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 10853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10853
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502999"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VFD79_03200"
     CDS             complement(<1..407)
                     /locus_tag="VFD79_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002836541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HZH93084.1"
                     /translation="MSRPIVCIVGRPNVGKSTLFNKLAGRRIAITEDKPGVTRDRIYA
                     EAEWLGQHFTVIDTGGLEPENEDVVMSNIRKQADIAIETADVILFVVDGIEGLTTTDR
                     EIGDLLRRSGKNVVLAANKIDTRKTPDEIYEFY"
     gene            complement(404..1741)
                     /locus_tag="VFD79_03205"
     CDS             complement(404..1741)
                     /locus_tag="VFD79_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HZH93085.1"
                     /translation="MEITNIKESRNIIEDVELGSIADELDVNPGDILLSINNREIKDV
                     IDYKYMMADEYLLVEIEKPRGEIWEFEIEKEPGEDLGIIFTNPLIDRAKSCNNKCMFC
                     FIDQLPPNMRDTLYFKDDDSRLSFLQGNFITLTNMGEDEIQRIIEYRLSPINVSVHTT
                     NPDLRVRMLRNKNAGKIFDILKRFHEVNIEMNCQIVLVPGVNDGEELKRTINDLSTLY
                     PSIHSVAVVPVGITKYRQGLDELEIYDGDTSAGVVLDVENMQRHFIEKIGTRYIFASD
                     EFYVVSGNKLPEYHEYEGFPQYENGVGLLRSFRSEVEEALEGSNLSSMDKKLIFATGT
                     LAYEFMRDIASMIMAKYDKLTIEVVPIKNYFFGETITVSGLVTGGDLVEQLKTYDGFE
                     KVIIPRSMLKRDEEIFLDNISLVQASDKLGIEIVPSGVDGKELINLIETGVRS"
     gene            complement(1807..3036)
                     /gene="glyA"
                     /locus_tag="VFD79_03210"
     CDS             complement(1807..3036)
                     /gene="glyA"
                     /locus_tag="VFD79_03210"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HZH93086.1"
                     /translation="MNFSNLKEADREIMEIVQEEINRQNKHIELIASENFVTEAVMEA
                     MGSQLTNKYAEGYPGKRYYGGCEVVDKAETLAIERLKQLFGADHANVQPHSGSNANLA
                     VYFAFLKPGDKVLGMKLSQGGHLTHGSPVNISGVYYNFVDYGVNSETETIDYDEVREI
                     ALKEKPKMIVAGASAYPREIDFSKFREIADEVGAYLMVDMAHIAGLIAAGLHQNPVPY
                     ADFVTTTTHKTLRGPRGGAILCKAEYAKAIDKAIFPGIQGGPLMHVIAAKAVAFGEAL
                     QDDFKTYQKQVVVNTNALAEGLLEEGFRLVSGGTDNHLILMDVRNKNLTGKDAEAMLN
                     DVGIATNKNTIPNDPESPFVTSGVRIGTAAMTTRGMKEAEMKEIAELMSEALKADSDK
                     EAVRQRVYDLCDRFPLY"
     gene            complement(3103..3363)
                     /locus_tag="VFD79_03215"
     CDS             complement(3103..3363)
                     /locus_tag="VFD79_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HZH93087.1"
                     /translation="MVLADKDSIIVRLRRVEGQIKGIQKMVDEDKFCGDILVQISAAR
                     SALNNVGGLILENYMKNCLLEAPDGSEEDLDKLIEIMLKYTK"
     gene            complement(3370..3792)
                     /locus_tag="VFD79_03220"
     CDS             complement(3370..3792)
                     /locus_tag="VFD79_03220"
                     /inference="COORDINATES: protein motif:HMM:NF016160.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HZH93088.1"
                     /translation="MDQIGFVIKKVDEQKVELEVKRASGCGSCNSCASSCEVKPHYVT
                     VKNNIDAKPGDFVELQAVQKNIMKYVTIIYMIPFAFLILGIVVGNSLFKGMDTSNHEL
                     MIFGMGILFLAISFVVVRIIDKVFAKKDESIIVMTRKL"
     gene            complement(3794..4507)
                     /locus_tag="VFD79_03225"
     CDS             complement(3794..4507)
                     /locus_tag="VFD79_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HZH93089.1"
                     /translation="MKSKFERSAYLLGENAMEKLSRATVAVFGIGGVGSYAAEALART
                     GIGKLVLVDYDIIDVTNINRQVHATTASVGYSKVEVMRKRLLEINPDLQVLVYNVKYS
                     AETRQELLSGDYDYIVDAIDMVSSKIDLIVNSKAMGIPIISSMGAGNKLNPTEFKVAD
                     IYETKICPLARVMRQELRKRGVDKLKVVYSEEEPMKVNLGEENRRKAVPGSVGFVPPV
                     VGLIIASEVIKDIAGQEER"
     gene            complement(4497..6284)
                     /gene="aspS"
                     /locus_tag="VFD79_03230"
     CDS             complement(4497..6284)
                     /gene="aspS"
                     /locus_tag="VFD79_03230"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HZH93090.1"
                     /translation="MVEKMGNLRRTKMCGSLRMEDAGSEVVLMGWVQRERNLGGLLFM
                     DLRDTTGMVQIVFDDEAPKEMMEKAETVRSEYVIAIRGRVRERQSKNPNIPTGDIEIL
                     ADEIRILDEAETPPIYIKDEDNVSETMRLKYRYLDLRKPSMQENLKLRANIAKVVRDF
                     FHENDFVEIETPMLTKPTPEGARDYLVPSRVNPGQFYALPQSPQLMKQLLMVSGMDRY
                     YQIVKCFRDEDLRANRQPEFTQVDVEMSFVDVEDVLEINEKLVQKLFKEIKGIDIELP
                     LKRMSYDEAMNRYGVDKPDLRFGLELRDASDILKGCGFKVFAETIETGGVVKGINVKD
                     GGDRFTRKDISKLEDHAKTYGAKGLAWIKISEDGTSSPIAKFFSDEEMKDLLEFFEGE
                     PGDLLLFVADSQDVANDSLGNLRNELAKRLDLISTDDYKILWITDFPLFEYDEEEDRY
                     VAKHHPFTHPVEEDVHLLESEPNKVRAKAYDLVINGDEMGGGSIRINNSELQSRMFSA
                     LGFSKEEAQSKFGFLLDAFKYGTPPHGGIAYGLDRLVMLFTGSTNIRDVIAFPKTQSA
                     TCLMTDAPTEASQKQLEEVHIKVDLKDEK"
     misc_binding    complement(6329..6518)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6548..8050)
                     /gene="hemZ"
                     /locus_tag="VFD79_03235"
     CDS             complement(6548..8050)
                     /gene="hemZ"
                     /locus_tag="VFD79_03235"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen dehydrogenase HemZ"
                     /protein_id="HZH93091.1"
                     /translation="MIFILVNDNKIKTHDPYELVRAFYGNEEIRIIGLDSVDSINPDS
                     DLISIFLHGDNYKVSLRRNKVVVDSEEMSYREKVFYRGETASRNNTVKHLIYKLLTRN
                     IEARPPWGILTGIRPVKVAKMLLDKGLGQEEAIEVLRDSYLLRPDKARLITEVSRTQR
                     PILDSLAKNSYSIYIGIPFCPTRCFYCSFPTLIANRNIHVMEGYVDSLIEELEDTKAM
                     LEGWHINTVYIGGGTPTSLPKELMGRLLDYINVNFKGFNELTVEAGRPDTIDRAYLEL
                     LKNKGVNRISINPQTMDDETLVLIGRKHTAKDIVEAYRLSKDIGFETVNMDLIVGLPG
                     ENRNNIAETLRAIRTLDPDNLTVHTLAVKKGSEFILDKNSYSLAESSEVEEMLQLTWE
                     FAEENNMIPYYLYRQKQIMGNFENIGYSKKHNPCLYNVSIMEEKETIVGIGMGSTSKF
                     YNKLENSIDTLANFRSITQYMERLPEIKRRKRQKINEIETSWNKIELDTP"
     gene            complement(8092..8721)
                     /locus_tag="VFD79_03240"
     CDS             complement(8092..8721)
                     /locus_tag="VFD79_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZH93092.1"
                     /translation="MIVKRLQAGVYAANCYIIHTEDGDGLVVDPAGDVDEIVKYLEDR
                     SVKVGAIVLTHGHGDHIGGVLELKELLDVPVMVHSDDEDMVKNSALNLSTSMPMGEVN
                     FKPDILLNDGDEIPLGDRAVKVIHTPGHTKGGVCILVDGILITGDTLFQGSIGRTDLY
                     GGDFDTLMISIVEKLMILPDDTVVYPGHGGPSTIGVERRNNPFISRWRG"
     gene            complement(8737..9186)
                     /gene="dtd"
                     /locus_tag="VFD79_03245"
     CDS             complement(8737..9186)
                     /gene="dtd"
                     /locus_tag="VFD79_03245"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HZH93093.1"
                     /translation="MRAVVQRITTGKVLVDDEIVGQIDKGLLVYLGVGENDTEVDLEY
                     MVNKVAGLRIFEDENGKMNLSVQDIGGEILAISQFTLYGDARKGKRPSFTDAASPEAG
                     DRLYEKFIARTSEMGIRTESGVFGAHMMVDYVNDGPVTILLDSTKLF"
     gene            complement(9200..>10853)
                     /locus_tag="VFD79_03250"
     CDS             complement(9200..>10853)
                     /locus_tag="VFD79_03250"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019034604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HZH93094.1"
                     /translation="LGISKIKWELEDLSLRYLDPDNYYDLVDKVSKRRLEREAYIDQI
                     IETLYEKLGEMGIKADINGRPKNFYSIYKKMMYQGKAFEQIYDLTAVRILVDNIKDCY
                     GALGIVHTLWKPLPGRFKDYVAMPKPNMYQSLHTTVMGNKGEIFEVQIRTYEMHRTAE
                     YGIAAHWKYKEGSPKADNFDEKLTWLRQLMEWQSDLNDPKEFMEALKIDFFTDEVFVF
                     TPRGDVINLPEGSTPIDFAYRVHTDVGNKCVGAKVDNRIVPLNHKLRNGNIVEVITSA
                     NSSGPSRDWLKIVKSNQAKTKIKQYFKLKERDRNVAKGKEYLEKEIKRLGYKPSDILR
                     DEWLRNIATKVSINSIEDLYASIGYGSITLNQVINKLKEIYSEHFKPEEKEIIESNIQ
                     KTARKPKPKHTQGIKVKGIDNIKVRFSKCCNPVPGDDIVGFITRGRGVSIHRTDCPNI
                     KSDLAEEERLIEVSWDFEKKASYNAEIQVRAADRPGLLAEIALRINDANVGLITLNAR
                     TSKDKTVLITMILEIHDKEQLNDLMKKVRKIGNVFDVYRVTA"
BASE COUNT         2730 a         2733 c         1804 g         3586 t
ORIGIN      
        1 tcataaaact cataaatctc atctggagtt tttctggtat caatcttgtt ggctgccaat
       61 acaacgttct tgccggacct tctcaaaagg tccccaatct ccctgtctgt tgttgtaagt
      121 ccctcaatac catccacaac aaatagtatt acgtctgctg tttcaatcgc aatgtctgcc
      181 tgcttcctga tgttgctcat tacaacatct tcattctcag gctccaatcc tccagtgtcg
      241 ataacagtaa aatgctgccc caaccattcc gcttcggcat atatcctatc ccgggtcact
      301 ccaggtttat cttcagttat tgctattctt ctgccagcta gcttattaaa caatgttgat
      361 ttccctacat ttggtctacc tactatacat actatcggcc tactcacgac ctcacccctg
      421 tctcaattaa atttatcagt tccttaccat ccactcctga tggtactatt tcaatgccta
      481 gcttatcact tgcctggacc aagcttatgt tgtctagaaa tatttcctca tctcttttca
      541 acatggatct tgggattatc accttttcaa aaccatcata ggtctttaac tgctcaacca
      601 gatccccacc tgtgacaagt cctgacactg ttattgtttc tccaaagaag tagttcttta
      661 tgggtacaac ctctatggtt aacttatcgt attttgccat tatcattgat gcaatatccc
      721 tcatgaactc ataggcaagt gtaccagttg caaaaatcaa ctttttgtcc atactggata
      781 gattcgatcc ttccaaagct tcctccacct ctgacctgaa tgaccttaaa agtcctaccc
      841 cattctcata ttgcgggaat ccttcatact catgatattc aggtagttta ttaccggaaa
      901 caacatagaa ctcatctgat gcaaagatgt atcgggttcc aatcttttca atgaaatgcc
      961 tttgcatgtt ctcaacatcc aaaacaacac ctgcagatgt atctccatca tatatttcaa
     1021 gctcatctaa accctgtctg tacttggtga ttcctacagg tacaacggca acagaatgaa
     1081 ttgatggata caaggttgaa aggtcattta ttgttctctt aagctcctcc ccatcattaa
     1141 ctccagggac aagtacaatc tggcagttca tctcaatgtt gacttcatgg aatctcttta
     1201 gtatatcgaa tattttccca gcattcttgt ttctgagcat tcttactctc aaatctgggt
     1261 ttgtggtatg gaccgataca tttataggac tcaacctata ctcaataatc ctctgtattt
     1321 catcctctcc catgtttgtc aaggtaatga aattaccctg caaaaaagaa agtctggaat
     1381 catcatcctt aaagtaaagt gtgtctctca tatttggtgg caattggtca atgaagcaaa
     1441 acatgcattt attgttgcat gacttagcac ggtcaattaa aggattagtg aaaattatac
     1501 ccaaatcctc tcctggctcc ttctcaattt caaattccca aatctctccg cggggctttt
     1561 cgatttccac caaaaggtat tcatctgcca tcatgtattt ataatcgata acatccttga
     1621 tttccctgtt gttgatactt aataggatat cccctgggtt cacatccagc tcatcagcta
     1681 tacttcccag ttccacatct tcaataatgt tgcgggattc cttgatattt gttatttcca
     1741 taataaacca cctgtctata aacattagtt aagcctacag cgagctgtag gcttattatc
     1801 actgaactaa tatagtggga acctatcgca aaggtcgtag actctctgcc ttacagcttc
     1861 cttgtcacta tcagctttta gagcttcaga catcagctct gctatttcct tcatttcagc
     1921 ttccttcatg cctcttgtgg tcattgcagc agtacctatt ctgacaccac ttgtcacaaa
     1981 cggactttca ggatcattag gtattgtatt cttgttagta gcaataccca catcgtttag
     2041 catagcctct gcatcttttc ctgtcaagtt tttatttctt acatccatca atattagatg
     2101 gttgtctgtt cctccagata ccaatctaaa gccttcctcc aacaagccct ccgctagggc
     2161 atttgtattt acaacaacct gcttctggta agttttaaaa tcatcctgaa gtgcttctcc
     2221 aaatgccaca gccttagctg cgataacatg catcaaggga ccaccctgaa tacccgggaa
     2281 gattgcctta tcaatggcct tggcatattc tgccttgcaa agtatcgccc ctcctcttgg
     2341 tcctctcagg gttttgtggg ttgttgttgt tacaaagtca gcgtaaggaa cagggttttg
     2401 gtgcaagcct gcagcaatca agccagcgat atgagccata tccaccatta gatatgctcc
     2461 cacttcatcg gctatttccc taaacttact gaaatcaatc tctcttgggt atgcacttgc
     2521 accggccaca atcatttttg gcttctcttt aagtgcaatt tcccttactt catcataatc
     2581 tattgtttca gtctcggaat taactccgta atctacgaaa ttgtaatata ctcctgaaat
     2641 gttgacaggg cttccatgtg taaggtgtcc accttgcgac aacttcattc ctagcacctt
     2701 gtctccaggc tttaaaaatg caaagtatac tgcaagattg gcgtttgatc ctgaatgcgg
     2761 ctgcacattg gcatgatcag caccaaacaa ttgcttcagc ctttcaatgg ccaaggtttc
     2821 agccttgtct acaacttcac atccaccgta gtatctcttt ccaggatagc cttcagcata
     2881 cttgttggta agctgacttc ccattgcctc cataaccgct tcagtaacaa agttttcaga
     2941 tgctatcaac tcgatgtgtt tgttctggcg attgatctcc tcctgtacaa tctccatgat
     3001 ttctcggtct gcttccttta gattactaaa attcatatcc cgactccttt caattgattt
     3061 ctattatacc aatttttagt ataatccgga tgattgtgga aattacttgg tatacttcaa
     3121 catgatttca atcagcttat caagatcttc ctcagaaccg tcgggagcct ctagaagaca
     3181 atttttcata taattctcta gaatcaatcc tccgacattg ttcaacgcgc ttctggctgc
     3241 tgatatctgt accagtatat ctccacagaa cttgtcttca tccaccattt tttgtatacc
     3301 ctttatttgc ccttctactc ttctcaacct tacaattatt gaatccttat ccgccaaaac
     3361 cacccctcct tatagcttcc ttgtcataac aataatgctc tcgtccttct tcgcaaaaac
     3421 cttgtcaatt attcttacaa ccacgaatga tattgccaga aaaagtattc ccattccaaa
     3481 gatcataagc tcgtgattag aagtgtccat tcccttgaat aagctattgc caactacaat
     3541 tcctaaaatg aggaatgcaa atggaatcat atatattatt gtgacatact tcatgatgtt
     3601 cttctgaact gcctgtagct caacaaagtc tccaggcttt gcatcgatgt tattcttaac
     3661 tgttacataa tgaggcttta cttcgcaaga acttgcgcag gaattacagc tgccacatcc
     3721 agaagctctc ttaacttcaa gctctacctt ttgttcgtct accttcttta ttacaaatcc
     3781 tatttggtcc aattcatctc tcctcctgtc ctgctatatc ctttattact tctgatgcta
     3841 ttatcaagcc tacaactgga ggtacgaacc ccacactccc agggaccgcc ttacgacgat
     3901 tctcctcccc tagattaact ttcatgggct cttcctcaga atatacaacc tttagtttgt
     3961 caacgcctct tttcctaagc tcctgtcgca tgaccctagc cagagggcaa atttttgtct
     4021 catatatgtc agcaaccttg aattctgtag gatttagctt gtttcctgca cccatggagc
     4081 ttattattgg tatgcccatg gctttggaat ttactatcag gtcaattttc gatgatacca
     4141 tgtctattgc atccacaata taatcataat cacccgataa cagttcttga cgcgtctctg
     4201 ccgagtattt tacattatac acaagaacct gtaaatctgg atttatttcc agaagtcttt
     4261 ttctcattac ctcaactttt gagtatccta cactagcagt ggttgcatga acctgtcggt
     4321 tgatattcgt tacatctatt atatcataat ctacaagaac tagcttgcca attcctgttc
     4381 ttgcaagcgc ttctgcagca tatgaaccta caccgccaat tccaaatacc gcaacagtag
     4441 ccctacttag tttttccatt gcattctctc cgaggagata tgctgatctt tcaaatttac
     4501 ttttcatctt tcaaatcaac cttgatatga acctcttcca gctgcttttg ggaagcctcc
     4561 gtaggagcat cagtcataag acaggttgca gactgagtct tgggaaatgc aattacgtcc
     4621 ctgatattgg tgctccctgt gaaaagcatc accaatctgt ccaagccata agcgattcca
     4681 ccgtggggtg gcgtgccata cttgaatgca tcgagcagga atccaaactt ggattgtgcc
     4741 tcttccttgc tgaaacccaa ggcactgaac atcctggact gcagctctga gttgttgatc
     4801 cttatgcttc ctccacccat ttcatcacca tttatgacaa ggtcatatgc cttcgcccta
     4861 accttgttag gctcgctttc aagcaggtga acatcctctt ccacagggtg ggtgaatgga
     4921 tggtgtttag ccacatatct atcttcttcc tcatcgtatt caaacagcgg gaaatccgta
     4981 atccaaagaa tcttgtaatc atccgtcgaa ataaggtcaa gtctctttgc aagttcattt
     5041 ctgaggtttc ctagactatc gtttgcaaca tcttgactgt cagctacaaa aagcaatagg
     5101 tcgcctggct caccctcgaa aaactcaaga aggtccttca tctcctcatc actgaagaac
     5161 tttgcaattg gggagctagt tccgtcctca cttatcttga tccaagccaa gccctttgca
     5221 ccataggtct ttgcatgatc ctcaagtttg ctgatatcct tgcgggtaaa cctgtcccca
     5281 ccatccttta cattgattcc cttgaccacg ccaccggtct ctatggtttc tgcaaatacc
     5341 ttgaagccac atccctttag aatgtctgat gcatctctaa gctccagacc aaatctcaag
     5401 tcaggcttgt ccacaccata cctattcatg gcctcgtcat atgacattct ctttagtggc
     5461 agttctatgt caattccctt tatttcctta aatagttttt gaacaagctt ctcatttatt
     5521 tccagtacat cttcaacatc cacaaaactc atctccacgt ccacctgtgt gaattcaggc
     5581 tgcctgttag ccctaaggtc ctcatccctg aagcacttga ctatctgata gtacctgtcc
     5641 ataccggaaa ccatcagaag ctgtttcata agctgtggtg actgtggtag tgcatagaac
     5701 tggccagggt tcaccctact cggtacaagg tagtcccttg ccccttcagg agttggtttc
     5761 gttagcatgg gggtttcaat ctccacaaag tcgttttcgt ggaagaagtc cctcacaacc
     5821 tttgcaatgt ttgccctaag tttaaggttt tcctgcatgg aaggttttct tagatcaaga
     5881 tacctgtact ttagtctcat cgtctcactg acattgtctt cgtctttaat gtatattggt
     5941 ggtgtttcag cttcgtcaag tattctaatc tcatctgcca gtatctcaat atctccagta
     6001 ggaatatttg ggttttttga ctgtctttcc ctaacccttc ccctgattgc aatcacatat
     6061 tcagatctta ctgtttcagc cttctccatc atttccttag gagcctcgtc atcaaaaaca
     6121 atctgaacca ttcctgtggt gtccctcaag tccatgaaga gcagtccccc aagatttctt
     6181 tctcgctgaa cccagcccat aagaacaacc tcactgcctg catcctccat ccttaagctt
     6241 ccacacattt tggttctcct taagttgccc attttttcta ccatatcaaa tacctcctta
     6301 aaataaataa agcccctcat ctctgatttt cagggacgag aggctatctc gcggtaccac
     6361 cctaattagg ttttcctcac tcaatctata acgtatttca cgagcagtta aactgctgac
     6421 tcaaaggtgt cttcacagga tattgatgct gaatttccac cgtcatcagc tctcttaaaa
     6481 caagtgatcc cattactttt ccttatcaaa gtctgtttat ttaatttaga taattataaa
     6541 ataatactta cggcgtgtca agttctatct tattccagga agtctcaatt tcattgattt
     6601 tctgcctttt tcgccttttg atttcaggaa gtctttccat gtattgcgtg atgcttctga
     6661 aatttgccaa agtatcaata ctgttttcaa gcttgttgta aaatttggaa gtggatccca
     6721 tgcctattcc aacaatggtc tccttttctt ccataatgga cacattgtat agacagggat
     6781 tatgcttttt actatagccg atgttttcaa agttacccat gatctgcttc tgtctgtata
     6841 gatagtaggg tatcatattg ttctcttctg caaattccca tgtcaattgg agcatttcct
     6901 ccacttctga ggattctgcc aatgaataac tgtttttatc aaggatgaac tccgacccct
     6961 ttttcactgc aagtgtatga acagtcaggt tgtcagggtc aagagtcctt attgccctaa
     7021 gggtttccgc aatattgttt ctgttttcac caggtaggcc gacaatcaag tccatgttta
     7081 ctgtttcaaa tccaatatcc ttacttagtc tgtatgcttc aactatatcc ttggcagtat
     7141 gcttcctgcc aataagaaca agcgtttcat catccattgt ctgtgggttt atgctgattc
     7201 ggttcacacc cttgtttttc aacaattcaa ggtaggcccg atctattgtg tcaggtctcc
     7261 cggcttcaac tgtcagctca ttgaaccctt taaaattcac attgatatag tcaaggagcc
     7321 ttcccatcaa ttcttttgga agggaggttg gtgtgcctcc accaatgtat acagtattta
     7381 tatgccaacc ttccagcatt gcttttgtat cttccagctc ctctatcaat gagtctacat
     7441 atccttccat cacatggatg ttcctgttgg caataagtgt tggaaaagaa cagtaaaagc
     7501 atctcgtggg gcaaaagggt attcctatat atatgctgta gctgttttta gccaatgaat
     7561 ccaggatagg cctctgggtt cttgatactt cagtaataag ccttgcctta tctggtctca
     7621 acaaataaga atcccttaaa acctcaatgg cttcttcctg tccaagaccc ttgtccaaaa
     7681 gcattttagc cacctttaca ggtctaatac ctgtaagtat accccagggt ggtctggcct
     7741 caatgtttct ggtaagaagt ttgtaaataa ggtgctttac cgtgttgttc cttgatgcag
     7801 tctcacctct gtaaaaaact ttctcccggt atgacatctc ttcagaatca acaactactt
     7861 tgttcctacg caatgatacc ttgtaattgt caccatgcaa aaaaatagaa attaagtctg
     7921 aatctgggtt tattgagtca acggaatcca gcccaattat tcgaatttcc tcattgccat
     7981 aaaaagccct tacaagttcg taagggtcat gtgtctttat tttattgtca tttaccaata
     8041 tgaaaatcat attcatcact ctaatcttga attattctac aataatgtgt cctaacctct
     8101 ccacctggag ataaatggat tattgcgtct ttcaactcct attgttgatg gtcctccatg
     8161 ccccggataa acaaccgtat catcaggtag gatcattagc ttttcaacta tgcttatcat
     8221 caatgtatcg aagtctcccc cataaaggtc cgtccttcca attgaccctt gaaatagagt
     8281 gtcacctgtt atcagaatcc catccaccag gatgcatacc ccacctttgg tgtgacctgg
     8341 tgtgtgaatc accttgacag ccctgtctcc aagtggaatt tcatctccgt cgttcaggag
     8401 gatgtcaggc ttaaagttta cctcccccat tggcattgat gtggacaggt ttaaagcgct
     8461 gtttttcacc atgtcctcat catctgaatg gaccatgaca ggtacatcca ataattcctt
     8521 taactccaga acacccccga tatggtctcc atggccatgg gtaaggacaa tggcacctac
     8581 cttgacactt ctatcttcca gatatttaac tatttcatcc acatctccag ctggatcgac
     8641 aacaagccca tcaccgtcct ctgtatggat aatatagcag tttgcagcat aaacccccgc
     8701 ttggagtctc ttaactatca tatgcgaatt tcctccttag aataactttg tactgtccaa
     8761 tagaatcgta acgggtccgt catttacata gtcaaccatc atatgggctc caaaaactcc
     8821 agactcggtt ctgattccca tttcagatgt acgggcaatg aatttttcgt acagtctgtc
     8881 acctgcctct ggtgaagctg cgtccgtaaa gctgggtctc ttgccctttc ttgcatcccc
     8941 gtacaaagtg aactgtgata tggccagaat ttctccacca atatcctgca ctgacaggtt
     9001 catctttcca ttctcatctt caaatatcct taatcctgcc accttgttta ccatatattc
     9061 aaggtcaacc tcggtatcat tttctccaac accaaggtaa actaaaagtc ccttgtctat
     9121 ctggcctact atctcatcgt ccacaagaac ctttccagtg gtaatccgct gtactactgc
     9181 tctcatatat tgacctcctt tatgctgtca ctctgtagac atcaaataca tttccaatct
     9241 ttctcacttt tttcattaga tcatttagct gttccttatc atgtatttcc agtatcattg
     9301 ttatgagaac agtcttatcc ttactggtcc ttgcatttag ggttatcagg ccaacattgg
     9361 catcatttat ccttaaggcg atttcagcca gtagtcctgg cctgtcagct gccctaacct
     9421 gaatttctgc attgtaggag gccttctttt cgaagtccca tgacacttca atcagtcttt
     9481 cctcctctgc aagatcagat ttaatgtttg ggcagtctgt cctgtgtata gagacacccc
     9541 tacctctggt aataaagcct acaatgtcgt ctcctggcac tggattgcag cactttgaaa
     9601 acctaacctt gatgttgtca attcccttga ccttgattcc ttgagtgtgc tttggctttg
     9661 gctttcttgc tgttttctgg atattgcttt ctatgatttc cttttcctca ggcttgaagt
     9721 gctcactgta aatctccttg agcttgttaa tcacctgatt tagggttatg cttccatatc
     9781 ctattgatgc ataaaggtcc tcgatggaat tgatgctaac cttggtagca atattcctaa
     9841 gccattcatc tcttagaata tccgatggct tgtacccaag acgctttatt tccttttcaa
     9901 ggtattcctt gccctttgca acgttcctgt ccctttcctt caatttgaag tactgcttta
     9961 tttttgtctt ggcttgattg cttttaacta tcttcagcca atctctactt ggaccgctac
    10021 tgtttgcgct ggtaattacc tctacaatat tcccatttct aagcttgtga ttcaaaggca
    10081 ctattctgtt gtctactttg gcaccaacac acttgtttcc aacgtcagta tggaccctgt
    10141 aggcaaaatc tattggtgtg gagccctcag gcaggttgat gacatctccc ctgggggtga
    10201 aaacaaaaac ctcgtctgta aagaaatcaa tcttaagggc ctccataaac tcctttggat
    10261 cattaaggtc gctttgccat tccataagct gtctcagcca ggtcagcttt tcatcaaaat
    10321 tatcggcctt gggactacct tccttgtact tccaatgggc cgcgatacca tattcagctg
    10381 ttctgtgcat ttcataggtc cttatctgga cctcaaatat ttcacccttg tttcccatta
    10441 ctgtggtgtg aagggactga tacatgttcg gtttgggcat ggccacgtag tccttgaacc
    10501 ttcctggaag tggcttccaa agggtgtgta caatgcccag ggcaccatag caatccttta
    10561 tgttatccac cagaatccga actgctgtaa ggtcataaat ctgttcaaaa gccttgccct
    10621 gatacatcat tttcttatag atgctataaa agttttttgg ccttccattg atatcggcct
    10681 ttatccccat ttctcccaac ttttcgtaga gggtttcaat tatctggtca atgtaggcct
    10741 ctctctccag cctacgttta gaaaccttgt caaccaggtc atagtaattg tccgggtcca
    10801 aatatctaag ggataagtcc tccagctccc attttatctt ggaaattcca agt
//
LOCUS       DASRQF010000125        10424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1021686, whole genome shotgun sequence.
ACCESSION   DASRQF010000125 DASRQF010000000
VERSION     DASRQF010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 10424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10424
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021686"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1579)
                     /locus_tag="VFD79_03255"
     CDS             complement(47..1579)
                     /locus_tag="VFD79_03255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZH93095.1"
                     /translation="MSEYDLSHFESKLIVRNIERKDFDEIIGLQKLCFPKMIPWERDQ
                     LSSHIRIFPEGQICVEYDGVIIGSSSSLIINFDEYNEQHSWDEITDEGYITNHDPDGY
                     NLYGMEVMVHPDYRNMKIGNRLYEARKELAEKLNLKSIIIGGRIPNYHNHSSELTPRQ
                     YVNEVIRHNIYDPILTFQLKNGFVLKRIIKKYLPEDKASLEYATLMEWSNIEYQGKSK
                     HQFKTAYPVRVSVIQYMMKKINSFEEFAHQCEYYVDVASNYHSDFVVFPEIFTTQLLS
                     FVDQDTPSQAVRVLTTFTDRYLEMFTHFAVRYNVNIIGGSHFVEEDGKIYNIAYLFRR
                     DGTIEKQYKLHITPNEKQWWGISTGDGIRVFDTDCGKIAILICYDMEFPELARIVTEK
                     GANIIFTPFCTDERQGYLRVRYCAQARAVENQIYTVIAGTVGNLTHVENMDIQYAQSA
                     IFTPSDFSFARDGIMAECTPNIEMVIVGDLDLELLKRTRKSGTVTLLSSRRSDLYELK
                     EK"
     gene            1859..3031
                     /locus_tag="VFD79_03260"
     CDS             1859..3031
                     /locus_tag="VFD79_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HZH93096.1"
                     /translation="MDIQKEALKLHSEYKGKIQVSSKMPIKSREDLSLVYSPGVAEPC
                     KKIMDDPSLAYEYTNKGNMIAVVTDGSAVLGLGNIGSRAGMPVMEGKAVLFKELGGVD
                     AFPICLESQDTEDIIFAIKQIAPSFGGINLEDIAAPKCFEIEGRLKEELDIPVFHDDQ
                     HGTAIVVLAAIFNSLKVVKKELGKVRIVINGSGAAGLAIAKLLIKAGAMDIIICDRKG
                     ILDPEDELIESSKKEIALITNPRKISGDLHDAMVDADIFIGVSAGNVVASEDVKRMNE
                     DSIILAMANPVPEIWPEDAKKGGARIIGTGRSDFPNQVNNVLVFPGIFKGALDVRASD
                     INEEMKLVAAYTIANMVPEHLMSEENILPDVLDRNVGKELAKAVAKAAKDSGVSRI"
     gene            3042..3572
                     /locus_tag="VFD79_03265"
     CDS             3042..3572
                     /locus_tag="VFD79_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93097.1"
                     /translation="MVEYIIKEAKDFYKVIKLKEFRKTEGVTFDIMVQSMIPKIDAID
                     RVIHQKGAISPGTVGTVEKAWNMHTHQEDNLLVLHGERYVELYQPDYGKIERFIVTPD
                     YIEHNGERILEGGGLVTWTTNVFHRITSGDDGSASINLATHLEGFDIKTNFNIYDLNV
                     ETGEYKLLREGYKDQK"
     gene            3778..4605
                     /locus_tag="VFD79_03270"
     CDS             3778..4605
                     /locus_tag="VFD79_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004838991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HZH93098.1"
                     /translation="MKLISWNIDSLNAALTSDSARALLSRDVLNTIKDQDPDVIALQE
                     TKLPAKGPTKKHVGILKDMFSDYEFVWNSSVEPARKGYAGTMFLYKKNLKPSVTHPEI
                     GAPGTMDSEGRIITLEFEDFFITQVYTPNAGDGLNRLEERQIWDAKYADYLADLDKVK
                     YVIATGDFNVAHKEIDLAHPDNNRNSAGFTDEERQGFTNLLAKGFTDTFRHVHGDVTG
                     IYTWWAQRVKTSKINNSGWRIDYWLVSDRIADKVIRSEMLDSGPRQDHTPLLLEIEL"
     gene            4944..5237
                     /locus_tag="VFD79_03275"
     CDS             4944..5237
                     /locus_tag="VFD79_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein, ric"
                     /protein_id="HZH93099.1"
                     /translation="MKKFNEMVEENLVRLGQYVPVVARVHGGSHPEFHDVKSTFDEMV
                     SKINNGGGETPDLEQEFKELRRITDNYTVPGDVCETYEAVYNMLEQLDRSYKK"
     gene            5270..7123
                     /locus_tag="VFD79_03280"
     CDS             5270..7123
                     /locus_tag="VFD79_03280"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010276737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZH93100.1"
                     /translation="MQRYILKSKNRITLLSGILIAIAFTSKWTAGNMDIFVWSLVIAS
                     IMGAAPIVIQAYQALRVKVVSIDVLVTIAVGGAFLIQNFEESAIVTFLFLFGAFLEQR
                     TLNQTRSAIKELTEMAPESALKQMKNGEFEEVQVEEVDVGDILLVKTGAKVPVDGTVL
                     TGEGSVNEASITGESIPVSKEKGSNVFAGTILDNGTVQIIADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKWYTPAVLVLAFIVWIFSRDIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARHGVLLKGSEVISDFSRLDTMIFDKTGTLTIGNPSVSEKVYYGEDMD
                     EVLGYLASVERESDHPLAKAVLDEIGETRFSTVTETEVVKGGGIVSRVNNHRIAVGNV
                     ALMEKENVFLSQTAKEDIERLEREGNSLVITSVNGELKVLMGIKDQIRPGVKEDLKAL
                     KKLGVKNLVMLSGDNQGTVDVVSKELGLTEAHGNMLPEHKSEYVKMLIEEKNQIVAFV
                     GDGVNDSPSLALANIGIAMGSGTDVAIETSDVVLMNSDFGRLPHALGLVKATARNMKQ
                     NILIAVGVVLILLASLLFSEWMNMSIGMLVHEASILVVILNGMRLLKYRLR"
     gene            7429..8646
                     /locus_tag="VFD79_03285"
     CDS             7429..8646
                     /locus_tag="VFD79_03285"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HZH93101.1"
                     /translation="MKRNTEVIHAQQKLDPVTGSHVSPLYQTSTYILPNYDEAVRLNQ
                     NIDQGFVYSRFGNPTVDEFEKKVAHLEKAEAALGAGSGLGAISTAVMSLVKAGDHIIF
                     GDVIYGCSFALFTKILPDFGVEYTIVDTTDVEAVKKAIKSNTTVIYVETPANPVLKIT
                     DIEEIAALKKDHPRIRLVVDSTFASPYLQNPLVHGADLVVHSATKYLNGHGTVTAGVI
                     AGSKKLIDRCRMPFMQCFGAVLDPFAAWTIMQGMKTLGVRMERHCQNAMEVAEYLDGH
                     PLVERVYYPGLESHPSHETAKKQMRSFGGMMSFDIKGGLESAKTVLNNVKIFSLATSL
                     GNVDSLIQHSPSMSHFDMTKEEREAVEIYDGQVRLSVGIEDVEDLIDDLEQALAKVEL
                     GATQDSEKKYALA"
     gene            8847..10058
                     /locus_tag="VFD79_03290"
     CDS             8847..10058
                     /locus_tag="VFD79_03290"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HZH93102.1"
                     /translation="MKSNKQGWIVVAAGAGINLAFGVLYAWSIFAGKLQETLGWTNTE
                     TSIPYTVAIIMFALVMIPAGRLQDKIGPKWVTTIGGLLIGLGCILAGSMTSILGLTIS
                     FGVLAGSGIGLGYASTTPPALKWFPPEKKGLISGIVVGGFGLATLYIVPLSNFLLSRY
                     DVFSSFRIMGVIFLIMTLPLAQLVKNPDKPVVSTSKDAKPPVEDRTVGEMLKTKQFYM
                     LWFMFFAGATGGLMTIGSLKPIVKAMLGESAALQLVAFAAVANALGRPMAGVISEKLG
                     RGKTMSILYVLQALALFLFNGLNSFATVLMAAAMIYFAYGSMLSVYPSACADNYGTKN
                     LGLNYGIMFSAWGAGGVFGPILGGRIADATGSYEMAFYVSAAILIVAAIMGLFYKTSQ
                     VKHSYVVTRNG"
     gene            complement(10129..>10424)
                     /locus_tag="VFD79_03295"
     CDS             complement(10129..>10424)
                     /locus_tag="VFD79_03295"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93103.1"
                     /translation="TDKVDAYSGYVNDLLPDLDLDWSETTALLCASPERIKKLARDLM
                     DLGMKPTNILVTLETHMRCGAGKCGHCKVGSHYMCVDGPVFTYEEMMALPNEY"
BASE COUNT         3173 a         1835 c         2507 g         2909 t
ORIGIN      
        1 cccttgtccc attctcccct tgtcttttat tcaatgttag aaatttctat ttctccttta
       61 gttcgtacag gtcagacctc ctgctgctga ggagagtaac agtacctgat tttctggtac
      121 gcttcaacag ttccagatcc agatctccaa caattaccat ttcaatgttt ggtgtacatt
      181 cagccatgat tccatccctg gcaaatgaga agtctgaagg tgtgaaaatg gctgactgtg
      241 catactggat gtccatgttc tcaacatggg taaggtttcc tacagtaccg gcgatgacgg
      301 tataaatttg gttttcaaca gcccttgcct gggcacagta tctgaccctt agatagcctt
      361 gtctttcatc agtacagaaa ggagtaaaaa ttatgtttgc gcccttctcg gtaactattc
      421 gggcaagctc aggaaactcc atatcatagc atatcaggat tgcaattttc ccacaatcag
      481 tatcaaatac cctgatgcca tctcccgttg aaatccccca ccattgcttc tcgtttgggg
      541 ttatgtggag cttatattgc ttttcgatgg tgccgtccct cctgaacagg taagcaatat
      601 tatagatttt tccatcttcc tcaacaaaat gggaaccgcc tattatgttt acgttatatc
      661 taactgcaaa gtgtgtgaac atttccaggt atctatcagt gaatgtagtt aggaccctga
      721 ctgcctgact gggggtatcc tggtcaacaa aggaaagcag ttgggtggtg aatatttcag
      781 ggaaaaccac aaagtcagaa tgatagtttg aagcgacatc aacataatac tcacattgat
      841 gggcgaactc ctcaaatgag ttgatctttt tcatcatata ttgaataacg ctgaccctta
      901 caggataggc ggttttaaac tggtgtttgc tcttgccttg gtactcaatg ttgctccatt
      961 ccatcaatgt tgcatattcc agtgaagcct tgtcctcagg caggtatttt ttaattatcc
     1021 tcttgagtac aaagccattc ttaagttgaa aggtcagaat gggatcatat atattatgtc
     1081 ggatgacctc attaacatat tgcctagggg ttaattcgga ggaatgatta tggtaatttg
     1141 gtattcttcc acctatgatt atgctcttta gattcagctt ttcagcgagc tcctttcttg
     1201 cttcgtacag cctgttccca atcttcatgt ttctataatc aggatggacc attacttcca
     1261 ttccatacag gttataacca tcaggatcat gattggttat gtagccttca tctgttatct
     1321 catcccatga gtgctgttcg ttgtactcat caaaattgat tatcaggctt gagcttgacc
     1381 cgatgatgac tccatcatac tcaacgcata tctgcccctc gggaaatatt ctgatatggc
     1441 ttgatagttg atctctttcc cagggtatca tcttagggaa gcatagcttc tgcaatccta
     1501 ttatttcgtc aaaatccttc ctctcaatgt ttctaactat tagcttgctt tcaaaatggg
     1561 ataaatcgta ttctgacata tttgacctcc ttgtgtttac atacttatgt atatacctat
     1621 acccttttaa gtccagctaa aatactattt ctgtcttggg ctgatcagat acaggaatgc
     1681 ggctggtttc tgttgttgct tgtcgtttct tttgatattc tgatagtgga ttactgaggt
     1741 aaacccttaa gtggaacggt gataacaaat agcagggtaa ggtcaagtca aatagggtat
     1801 agtttgattg tagacatatt ttcaagcttt ataaaaatat tgttaagggc ggtggattat
     1861 ggacatacaa aaggaagcgc tcaaattaca tagtgaatat aagggaaaga tacaggtctc
     1921 gagcaaaatg ccaataaaga gcagggaaga tctcagtttg gtttacagcc caggtgttgc
     1981 agagccttgt aagaagatta tggatgaccc ttcccttgct tatgaatata cgaataaagg
     2041 caacatgatt gctgtagtca cagatggatc agccgtactg ggtttaggca acatagggtc
     2101 cagagcaggc atgcctgtaa tggagggcaa ggcagttttg ttcaaggagc tggggggtgt
     2161 ggatgctttc cctatatgcc tggaaagcca ggatacagag gatataatat ttgccatcaa
     2221 gcagattgca ccaagttttg gagggataaa cctggaggat atagccgctc ccaaatgctt
     2281 tgagattgaa ggaagactaa aggaagagct ggatatacca gttttccatg atgatcaaca
     2341 tggaacagct attgttgttc ttgccgccat ttttaattca ctgaaggtgg tgaaaaagga
     2401 acttgggaag gtgagaatag tgataaacgg ttctggtgca gctgggttgg caatagctaa
     2461 gctgcttata aaggcaggag ccatggatat aataatctgc gatagaaagg gcatattgga
     2521 cccggaagat gaacttatag aaagcagcaa gaaggaaatt gcattgatca ccaatcctag
     2581 gaaaatatca ggtgatttac atgatgccat ggttgatgca gatatattca taggggtttc
     2641 tgcagggaat gttgttgctt ctgaggatgt taaaaggatg aatgaggatt cgattatttt
     2701 agctatggct aatccagttc ctgaaatatg gccagaggat gccaagaaag ggggggcaag
     2761 aataattggg actggaaggt cagactttcc aaaccaggtg aacaatgtcc ttgtttttcc
     2821 tgggattttc aaaggggcac tagatgtaag ggcaagtgat ataaatgagg agatgaagct
     2881 tgtagctgct tacactattg caaacatggt accggagcat cttatgagtg aggaaaatat
     2941 tttgccggat gtcctggata ggaatgtggg taaagagctt gcaaaagctg tagccaaggc
     3001 tgcgaaggat agtggagtgt ccagaatata aggagggtga tatggtggag tacattatca
     3061 aggaagcaaa ggatttttac aaggtgatta agcttaagga gttcagaaag accgaagggg
     3121 tgacctttga tattatggtc caatcgatga ttccaaaaat tgacgctatc gatagggtta
     3181 ttcatcaaaa aggtgccatc tccccaggaa cagttggtac agttgaaaag gcctggaaca
     3241 tgcacacaca tcaggaggac aatctattgg tcctacatgg ggaaaggtac gtggaactct
     3301 accagccaga ctatggtaaa attgaaaggt tcattgttac ccctgactac attgaacata
     3361 atggggagcg gattctggaa ggtggaggtc tggtaacctg gactacaaac gtatttcata
     3421 ggattaccag tggagatgat gggtctgctt caataaacct ggcaacacac cttgaaggtt
     3481 ttgatataaa gactaacttt aacatttatg acctgaatgt tgaaaccggg gaatataagt
     3541 tactaaggga aggatacaag gaccaaaagt agatggggat tcgctgattt ggaataagaa
     3601 aatatgaagt caaaatgatt tattaaaagc aataggagcc cttcctattg ctttttaaag
     3661 gtcataatca gtaaaaaatg aggatttgtt aggtatatgc taatatggta caatatttgt
     3721 aagtttgtcg aaaaacaata atgaatataa taataatgat gaaaaatgga gggaacaatg
     3781 aaattaattt catggaatat cgattcatta aatgctgcac tcacaagtga ttcagcccgc
     3841 gccttgttat ccagggatgt gctgaacaca atcaaagacc aagatccaga tgtcatagcc
     3901 ctgcaggaaa ccaagctacc agccaaggga cccactaaga agcatgttgg gattttgaag
     3961 gacatgtttt ctgactatga gtttgtctgg aacagttctg ttgaaccagc ccgtaaggga
     4021 tatgcaggta caatgttcct ttataagaag aatctgaaac catctgttac ccacccggaa
     4081 attggagcac ctggcaccat ggattctgag ggccgaatca taacattgga gtttgaagat
     4141 ttcttcataa cacaggtcta cacaccaaat gctggggatg gattaaatcg tttggaggaa
     4201 cgtcaaatct gggatgccaa atatgcggac tatcttgcag atttagataa ggttaagtat
     4261 gttattgcta caggtgactt caacgtagct cataaggaga tagatctggc tcatcctgat
     4321 aacaatagaa attcagccgg attcacagac gaggaacgtc agggcttcac aaatcttttg
     4381 gcaaaaggct ttactgatac atttagacat gtccatggtg atgtgactgg catctatact
     4441 tggtgggcac aacgtgttaa aaccagtaag atcaacaatt caggctggag aattgattat
     4501 tggctggtta gtgaccgtat agcagacaag gtaatcagat ctgaaatgct tgattctggc
     4561 cctagacagg accatacacc tctactgctt gagattgaac tgtaaagtag gggatagtat
     4621 tagggggaag ggaatacttt taataacact tggctgtata attcccaaga taagctcttg
     4681 agtcctgagt ccatgttgat ggactcagga ctttttaaat taaaacattt cccaatgttg
     4741 acctggatca aatactttta ggaaatataa tgttaaggta tatacataag ctggatatgg
     4801 ttgaggatca agaaattaca cagaaacaca aataggattt tgaattttga ctttggtcaa
     4861 ggacttatgg tgccaggcac ggtatcatgt agttaaggaa agtgattaag tgaaatatta
     4921 taaaatttta aggagagaat acaatgaaaa aattcaatga gatggtggaa gaaaacttgg
     4981 taagacttgg acaatatgta ccagtggttg caagagttca tggtggtagt cacccagaat
     5041 tccacgatgt aaaaagtaca ttcgatgaaa tggtttcaaa gatcaacaat ggaggaggag
     5101 aaactcctga tctggaacaa gagtttaaag aattacgaag aattacggac aattacactg
     5161 tacctggaga tgtatgcgaa acttatgaag cggtctacaa catgttggaa caactagata
     5221 gatcttacaa gaaataagat cgaaaagcaa ggattcagga ggggtaatga tgcaaagata
     5281 tattttgaaa agcaaaaaca ggattacact attaagtggt attttgatag ccattgcctt
     5341 tacaagtaaa tggacagcag gaaatatgga tatttttgtt tggtcattgg tgatagcttc
     5401 aatcatggga gcagcgccaa tagtgattca agcataccaa gcattgagag taaaagtagt
     5461 gagcatagat gtactggtaa caattgcagt gggtggagca tttctaatac aaaacttcga
     5521 ggaatcagca attgtaacct tcctcttcct atttggtgcc ttcttggagc agcgtacatt
     5581 gaaccaaact cgttctgcca taaaggaatt gacagagatg gcgccggaaa gcgcactgaa
     5641 gcaaatgaaa aatggagagt ttgaagaggt ccaggtggaa gaggtagatg taggcgatat
     5701 tttgcttgtt aaaacgggcg caaaagttcc agtggatgga acggtgttaa ccggagaagg
     5761 cagtgtcaac gaagccagta tcaccggaga atccattcca gtgagcaagg aaaaggggtc
     5821 aaatgtattt gcagggacaa tccttgataa tggtacggtt caaatcatag cggatagagt
     5881 cggtgaagat acaacatttg gaaaaataat tgagctggtt gaggaagctc aagattcgaa
     5941 atcagaggca gaaagattta tagatagatt ttcaaagtgg tatacaccag cagtcctggt
     6001 tttagcattt atagtatgga tattttcaag agacatagag ctggcaatca caatactggt
     6061 actgggttgt cctggagcat tggtgatagg ggtaccggta tccaacgtag caggaatcgg
     6121 aaatggagca cgtcacggag ttctattgaa gggtagtgaa gtgatcagcg atttcagtag
     6181 gctagacaca atgatatttg acaagactgg tacacttaca ataggcaatc catctgtatc
     6241 agaaaaagta tattatggag aagacatgga tgaggtacta ggttatttgg caagtgtaga
     6301 aagagaatca gaccacccat tggcaaaagc ggtcttggat gaaatcggag aaacaaggtt
     6361 ttccacagta actgaaacag aagtagtaaa gggtggagga attgtatcaa gagtaaataa
     6421 tcatagaata gcagtgggta atgtagcact gatggaaaaa gaaaatgtct tccttagcca
     6481 aacggctaag gaagacatag aaagattgga aagagaaggg aattccttgg ttataacatc
     6541 agtgaatgga gagttgaagg tactgatggg aatcaaagac cagatccgtc caggtgttaa
     6601 ggaagacttg aaggcactga agaaactggg agtaaagaac ttggtcatgc tttcagggga
     6661 caatcaagga accgtggatg tggtaagcaa agagctgggc ttgactgaag cccatggcaa
     6721 tatgctgccg gagcataagt ctgaatatgt aaaaatgttg atcgaagaaa agaaccagat
     6781 tgtagcattt gtgggggacg gagtcaatga cagcccatca ctagccttgg caaacattgg
     6841 aattgcaatg ggaagtggta cggacgttgc catagaaacc tccgatgttg tactgatgaa
     6901 ttcagacttt ggccgtttgc cacacgcctt gggactggta aaagcgactg caaggaatat
     6961 gaaacagaac attcttatag cagtgggcgt ggtactgatt ttgctggcaa gtctactatt
     7021 tagcgaatgg atgaatatgt ccataggaat gttggtgcac gaggcgagta tattggtagt
     7081 tatcctaaat ggtatgaggc tactgaagta taggttaaga tgagagtagg aagtgtttat
     7141 ttaaggtgaa gtcttaagtt gattattatt tcccgaaaat tccaacattt tgatattgac
     7201 aatcctgtat caatcaccta caatggagat tggtgcgcct gaccataatg actcagtttg
     7261 ggtgaagttt gaataatagc ttcgacaaaa cgagatgtcg tatagtcaag acgctatgga
     7321 atacacctag agctgttatc tttgctaaag gagtatctca tgcagtctga aaaacccata
     7381 gtagtaaatc ttggtgtagg atgcaaatat gacttggagg gaattgtaat gaaaagaaat
     7441 actgaggtga ttcacgctca acaaaaattg gacccggtca ccggatccca tgttagtcct
     7501 ttgtatcaga cttcaaccta tatactaccg aactacgatg aagccgtaag gctgaatcag
     7561 aacatcgacc agggctttgt atacagcagg ttcggaaatc caacagtgga tgagtttgag
     7621 aagaaggttg ctcatttgga gaaagccgaa gcggctctgg gagctggctc aggacttggg
     7681 gccatttcca cggcagtgat gtccctggtt aaggcaggag atcacattat tttcggagac
     7741 gttatctacg gatgtagttt cgcattgttt acaaagattt tgcctgattt tggtgtggag
     7801 tacaccatag ttgatacaac tgatgttgaa gcagtaaaaa aagccattaa atccaacacc
     7861 accgtaatct atgtggagac tccggcaaat ccggtattga agattacaga tatagaagaa
     7921 atcgcagcct tgaagaagga tcatccaagg atacgtttgg ttgttgacag caccttcgca
     7981 tcaccgtatc tacagaatcc actggttcat ggtgcagacc tggtggttca cagtgctaca
     8041 aagtatctaa atggacacgg aactgtaaca gctggtgtga ttgcaggaag caagaagctt
     8101 attgacaggt gtaggatgcc attcatgcag tgttttggag ctgttcttga tccttttgca
     8161 gcatggacaa tcatgcaggg tatgaaaacc ctgggagtcc gtatggaaag acattgtcag
     8221 aatgccatgg aggttgctga gtatcttgat ggccaccccc ttgttgaaag ggtatattat
     8281 cctggacttg aaagtcaccc tagccatgag accgctaaaa agcagatgag aagctttggt
     8341 ggaatgatga gctttgacat aaaaggtgga ttggaatctg ccaagactgt ccttaacaat
     8401 gtaaagattt tcagtctggc aacaagcctg gggaatgtgg attccttgat tcagcactca
     8461 ccatccatga gccatttcga catgaccaag gaggaaaggg aagctgtgga gatttatgat
     8521 gggcaggttc gtttatctgt tgggattgaa gatgttgagg acctgattga cgatctggaa
     8581 caggctttgg ccaaggtgga gctaggagcg acacaggatt ctgaaaaaaa gtatgcactg
     8641 gcttaaaata gtcaaataac agactccttt tgcggtagac agcttgaaga acaaggtgtc
     8701 tactgcaatt aggagtattt tttttcaaac tacattcgat tttgaaaaaa gtttgttatt
     8761 ttattgatta tatcattgta agttaaggtc aaataagcta taattggacg tcggagactt
     8821 aaaaaaatta ttaaggggga caaaatttga aatcaaataa acaaggctgg attgtagtag
     8881 cagccggagc tggaattaat ctagcctttg gtgtgcttta tgcctggagc attttcgcgg
     8941 ggaaacttca ggaaaccttg ggatggacca atactgagac ttccattcca tatacagtag
     9001 caattataat gtttgcattg gtaatgattc ccgcaggacg tctgcaggat aaaataggac
     9061 ccaagtgggt tactacaatt ggaggattac ttatagggtt gggttgtatc ttggcaggtt
     9121 ccatgacaag catactgggc ttgaccatct cttttggagt tttggcaggt tcgggaatcg
     9181 gcttgggata tgcatccaca acaccaccgg ccctaaagtg gtttccacct gagaagaagg
     9241 gactcatatc agggattgtc gttggaggat ttggacttgc aaccctatat atcgtaccac
     9301 tgagcaattt tctgctttca aggtatgatg tattttcatc attcagaatc atgggtgtaa
     9361 tattcctgat tatgacattg cctcttgcac aattggtgaa aaatcctgat aaaccagtgg
     9421 tttcaacaag taaggatgca aaacctccag ttgaagacag gactgtaggt gagatgctta
     9481 agacaaagca gttctatatg ctttggttta tgttctttgc aggagcaaca ggtgggctta
     9541 tgacaattgg tagcctaaaa ccaatagtaa aagccatgct gggtgaaagc gcagctcttc
     9601 agctggtggc ttttgcagcc gttgccaatg cacttggcag accaatggca ggagtaatat
     9661 ctgaaaagct tggcaggggc aagacaatgt caattcttta tgtgttgcag gcgctggcct
     9721 tgttcctttt caatggactg aatagttttg ccactgtttt gatggcagca gctatgatct
     9781 attttgccta tggatccatg ctttccgtct atccttctgc atgcgcagac aactacggaa
     9841 cgaaaaatct tggtctcaac tatggaatca tgttctcggc atggggtgca ggtggtgtct
     9901 ttggtcctat tctaggtgga agaatagccg atgccacagg gtcttatgaa atggcatttt
     9961 atgtgtcagc agcaatcctt attgtagctg caatcatggg acttttctat aagactagtc
    10021 aggttaaaca ttcctacgta gttacaagaa acggttaaaa aacatactaa taaaaaaggc
    10081 tctgccaaac tcgaatcatg agtggcagag cctttttaat gcattattct aatattcatt
    10141 tggcagtgcc atcatctctt cataagtgaa tactggaccg tccacgcaca tatagtggct
    10201 tcctaccttg cagtggccac atttacctgc accacaacgc atatgtgtct caagggtaac
    10261 aagtatgttt gtgggtttca ttccaagatc catcaggtct cttgccagct tcttgattct
    10321 ctccggtgat gcgcaaagaa gtgccgtagt ctccgaccag tcaaggtcaa ggtctggaag
    10381 caaatcattt acataaccac tgtatgcatc caccttatct gttg
//
LOCUS       DASRQF010000126         5800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_199940, whole genome shotgun sequence.
ACCESSION   DASRQF010000126 DASRQF010000000
VERSION     DASRQF010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5800
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199940"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..801
                     /locus_tag="VFD79_03300"
     CDS             184..801
                     /locus_tag="VFD79_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZH93104.1"
                     /translation="MSSNVKFIKTHDIDLDKINVRRAHEGDEEGIYKIACTVTEGERD
                     SYQGFLIDDYHKYPEYYIDLFRDRIRNLRYFYVAELGDRIIGFSLAYKKEMWIKYNPT
                     WLEDIHWHPSFDKSLLDDFILIDKTAIEAEYTGEGIGSKIYRRIIEDMRENDIHYIFS
                     ETVVNPLPNFASLAFRQKQNYIMAGFRYEEHKGVIYSDMVYYKKI"
     gene            complement(839..1042)
                     /locus_tag="VFD79_03305"
     CDS             complement(839..1042)
                     /locus_tag="VFD79_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HZH93105.1"
                     /translation="MENGVEFEYIDITDSMRSLKIFLAFRDKFEYFDPIRKKGSVGIP
                     TIMIGHGEEFMEGVPGMDLSRLK"
     gene            1237..2787
                     /locus_tag="VFD79_03310"
     CDS             1237..2787
                     /locus_tag="VFD79_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HZH93106.1"
                     /translation="MTYSPSLGSSLTNTRMRTPDNLSPFSGMCSVCTANCIGMCEIGL
                     SALRGKEATYPFQTDSSQFASEKDYPVDYSHLNINGRVFGAWGCKEDPYEATYPKANM
                     EARVGIENPVKLKMPIILPAMAKLNWRDYYAGAAMAGVLVVIGEDVIMKDPDLVLEDG
                     KVKSAPLIGEMLDSFRKYNRGYGDIVLQGNYDDEYNGVLDYAIKDLGATSVELKFAQA
                     AKGIQGLGMVNTLEDALKLKKRGYIVHPDPEDDEVAEKYKVGIGQRFEKVGKLPIWNE
                     DHLVGRVGELKEMGAKRVFFKTGPFEPSDLAKILMIASKAGVDLVTFDGAGGGTGNSP
                     VKMMNEWGMPTIVLESILYSLLKEMENKGHRLPQVAITGGIAMEDQVFKALALGAPYI
                     NMVGIGRAAMTAAMVGKTIGDLLEEGKVPKEFQRFGETKEDLFEGLRDLRQYYEDADT
                     FSTGAIGLYSYLGRIKAGTQQLMALNRKFDLKSIAREDVVPLTFEAKQATGLKDYKDL
                     LQEGLKLL"
     gene            2918..3883
                     /locus_tag="VFD79_03315"
     CDS             2918..3883
                     /locus_tag="VFD79_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZH93107.1"
                     /translation="MRSKTKFLSMLMVLLLSFSVLTACSPAAPAEEPAPPAEEPAEEP
                     AEEPAEEPAEEPALDPEEVLREAALDLLNNIPDNNFIMASDEALSLFEENPGAVFWVD
                     LRSPDDYAAGHITGAVNIPFAELGSHWDVLPSNRQIIMQCYSGQTSAQATALAQMLGF
                     NAVSFRGGMNFGWAPLELGEDTLEMEANDLPQASAPDLDEREQIIWDAAVAYFPPAAN
                     NIISPEDLLALVEENPDAITVVDIRSADDYAAGHIEGAIHIPFKEVGANIDQIPMNRP
                     AYVTCYTGQTAGITIAPMRMMGYNAISLSRGMTGWDGAELPKVMD"
     gene            3924..4193
                     /locus_tag="VFD79_03320"
     CDS             3924..4193
                     /locus_tag="VFD79_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93108.1"
                     /translation="MSKPKIFNSWLGSLAVVAVLAIVAIGIYQIGILPGLPEEEYTPV
                     GGGEDKPFEFPSVDSASCVACHTSEAVIAASTWVEEQPVAEDTGG"
     gene            4221..5294
                     /locus_tag="VFD79_03325"
     CDS             4221..5294
                     /locus_tag="VFD79_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HZH93109.1"
                     /translation="MEMFLVSQEFVDSTHGSLGCITCHGGANEVDKAVAHEGMEPYPS
                     RNFEANCGACHSNVTDIYATSIHYNLHGMENGLAAFADVMSLSESPHHEEVFDQDCFK
                     CHATCGDCHVSRAKNFSNGLVDQHTFLGTPSMEENCYACHNARNAGEYMGLIGFGGDV
                     HYEAGLTCMDCHPVDNFHGSETATKNMWEENLPACTDCHTDKDPAVATDVNHKVHGDS
                     LSCQVCHAQANNNCFECHLDPKEDGSGLQSSSESKIMFRVGLNPNPTEERPYKYVALR
                     HVPGQEAMLDVVGENMMPNFNEITNWKYSPTHNIQASTFQNESCESCHENDRIWLSED
                     DFRETDSEANFRLIPERPPSLDH"
     gene            5356..>5800
                     /locus_tag="VFD79_03330"
     CDS             5356..>5800
                     /locus_tag="VFD79_03330"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HZH93110.1"
                     /translation="MTIDNFIRRLTSLGLAITLFIFIAAYSIIGTVLPQGLQREFYME
                     SYPSMGSIITSLQFDQVYSSWIFRILLFLFIVNLSGCTLKILPGQLKKMNKDHFPKPK
                     PDGDNLHAENMDIQKFRKILEKKRYAITEDEKGFKASKHRIGNIGS"
BASE COUNT         1773 a         1175 c         1456 g         1396 t
ORIGIN      
        1 aaactatcgg aattgattaa aaagttaata tttgccatca aaaccccaat tatatgatta
       61 ttccgtggaa tatcttcaat ttgcttcagt tattgatgtt tttagtgtaa aataaaagaa
      121 gaaaagctgg tttaggctct aatagggcta gagggaaagg cattgattaa aggaggtggc
      181 tgcatgagtt caaatgtaaa gtttatcaag acccatgata tagaccttga taagataaat
      241 gttagaaggg cacatgaagg tgacgaagag ggaatataca agattgcatg tacagttaca
      301 gaaggggaaa gggactcata ccagggcttc ctcattgatg actaccacaa gtatcctgag
      361 tattacatag accttttcag ggataggatc aggaacctaa ggtattttta tgttgccgaa
      421 ctgggtgaca ggataattgg attttcattg gcatacaaaa aggagatgtg gataaagtac
      481 aatccaacct ggcttgagga catacactgg catccttcct ttgacaagtc attgctggat
      541 gatttcatcc tgatcgacaa gactgcaatt gaagcggaat acacagggga gggaatcggc
      601 agcaagatat acaggaggat aattgaggat atgagggaaa atgacatcca ctacattttc
      661 tctgaaacag tggtgaatcc cctgccaaac tttgcatccc tggccttcag gcaaaagcag
      721 aactacatca tggccggctt taggtatgaa gagcacaagg gtgtaatcta ttcggacatg
      781 gtctactaca agaaaatatg atcaaaataa aaatagggtc tgacaaatat gtcagcccct
      841 attttaatct ggaaaggtcc attcctggaa caccttccat aaattcttct ccgtgtccaa
      901 tcatgattgt tggtatacca acgctgccct ttttcctaat gggatcgaaa tactcaaatt
      961 tgtccctgaa tgccaggaat atcttaaggc ttctcatgct gtcagtaatg tcgatatact
     1021 caaactcaac tccattttcc aagagaaact ctctcagtgg ttcacactcc ggtcaaaggg
     1081 gacttccgaa aactgttatc cttttcatat aaaaaacctc ctcaatatgt cttgattata
     1141 acatatattt atccatagtt gaaattgaca tatgtcagaa aagggcgtaa tattaaggtg
     1201 tagactatat caatttaaag tagaggagga acaacaatga cctatagtcc aagcttggga
     1261 agctcattaa caaataccag gatgagaact cctgacaacc tttctccatt ctctgggatg
     1321 tgctccgtat gtacggcaaa ctgcataggg atgtgtgaga tcggcctttc tgccttaagg
     1381 gggaaggagg cgacctaccc cttccaaacg gacagtagcc agttcgcatc ggagaaggat
     1441 tatccggtgg actactctca tctcaacatc aatggaaggg tgtttggtgc ctggggttgc
     1501 aaggaagacc cctacgaggc cacctacccc aaggcaaaca tggaagcaag ggttggaatt
     1561 gaaaatcctg tgaagcttaa gatgcccatt atcctgccgg caatggcaaa gcttaattgg
     1621 agggactact atgcgggtgc tgccatggca ggagtccttg tggtgatagg agaggatgta
     1681 ataatgaagg atccagacct tgtacttgaa gacggcaagg taaaaagtgc acccttgata
     1741 ggcgagatgc tggattcatt caggaaatac aacaggggct atggagacat agtgcttcag
     1801 ggaaactacg acgatgagta caacggtgtc ctggattatg ccattaaaga ccttggtgca
     1861 acttccgttg agttgaagtt tgcccaagct gcaaagggga tccagggact tggtatggtc
     1921 aataccctgg aagatgccct aaagcttaag aaaaggggat acattgtcca tcctgaccct
     1981 gaggatgatg aagttgcaga aaagtacaag gtaggaatcg gtcagaggtt tgaaaaggtt
     2041 ggaaagcttc ccatatggaa tgaggaccac cttgtgggac gtgtaggaga actgaaggaa
     2101 atgggagcca agagggtctt cttcaagaca ggtccctttg aaccctcaga ccttgcaaaa
     2161 atactcatga tagcatcaaa ggcaggagtt gaccttgtca cctttgatgg agcaggggga
     2221 ggaacaggaa acagccctgt gaagatgatg aatgaatggg gaatgcccac gatcgtcctt
     2281 gagagcatcc tctattcact cttgaaggaa atggaaaaca agggccacag gctaccacag
     2341 gttgcgatca ctggtggaat tgccatggag gaccaggttt tcaaggccct ggcactggga
     2401 gctccataca tcaatatggt gggtatcggt agggctgcaa tgacggcggc aatggttgga
     2461 aagaccattg gagacctgct tgaggaagga aaggtcccca aggaattcca aagatttgga
     2521 gagaccaagg aagacctgtt tgagggactt agggacctga ggcaatatta tgaggatgcg
     2581 gacaccttct ccacaggagc aataggactt tactcctatc ttggaaggat taaagccggg
     2641 actcaacagc tgatggcact taacaggaag tttgacctaa agagcatagc ccgtgaggat
     2701 gttgttcctc taacatttga ggccaagcag gcaacgggac ttaaggatta caaggacctg
     2761 ttacaggaag gattgaaatt attgtaattg tgtgacaatt acacataaat gttgacatat
     2821 gaccataaaa tgctatactt taaggtaacg ggaatatgtc atctatcctg ccaaagtaag
     2881 ttaaaaattt aactaacaaa tttaaggggg agtttctatg agaagtaaga ctaagttttt
     2941 aagcatgctg atggtacttt tgctttcatt ttcagtactg actgcatgtt cacctgccgc
     3001 accggccgaa gagccagcac ctccagcaga agagccagcg gaagagcctg ccgaggaacc
     3061 tgccgaggag ccagcagagg agcctgcact ggatcctgag gaggtactta gggaagcagc
     3121 gcttgattta cttaacaaca ttccagacaa taattttata atggcatcag acgaagcttt
     3181 atccttgttt gaggagaatc cgggagcagt tttctgggta gacctaagat ctcctgatga
     3241 ctatgcagct ggtcacatta caggtgctgt aaacattcca tttgcagagc tgggaagtca
     3301 ctgggatgtt cttccatcaa acaggcagat tataatgcaa tgctattcag gacagacttc
     3361 tgcacaagcg acggcactag ctcagatgct tggattcaat gcagtttcct ttagaggtgg
     3421 aatgaacttc ggatgggctc cattggagct gggcgaggat accttggaaa tggaagccaa
     3481 tgacctgcca caagcaagtg caccggacct ggatgaaaga gagcagatca tatgggatgc
     3541 agcagttgct tacttcccac cagcagctaa caacatcata tcaccggagg accttcttgc
     3601 tcttgttgag gagaatccag acgccataac tgtggttgat atcaggtcgg ctgatgacta
     3661 tgctgcagga cacattgagg gagccatcca tatacctttc aaagaggttg gagcaaacat
     3721 cgatcagata ccaatgaaca gaccggccta tgtaacctgc tatacaggac agacagctgg
     3781 aatcacaata gctccaatga gaatgatggg ctacaatgca atcagtctaa gcagaggaat
     3841 gacaggctgg gacggagctg agctacctaa ggtgatggat tagaaagagg ctgtaaagat
     3901 ctacttaaca gggggtgaga tctatgtcaa aacccaaaat cttcaatagc tggttaggga
     3961 gccttgcagt ggttgcagtt cttgcaatcg ttgccattgg aatatatcag attggaatcc
     4021 tgcctggatt gccagaggag gaatatactc ctgtcggcgg aggggaggac aaaccatttg
     4081 agtttccatc agttgacagc gcaagctgtg ttgcctgcca taccagcgaa gcagtgatag
     4141 ctgcatccac ctgggttgag gaacagcctg tcgcggaaga cacagggggc tgaggggccc
     4201 cagggccaca gctggaagct gtggagatgt ttcttgtatc acaggagttt gttgactcca
     4261 cacacggaag tcttggctgc ataacctgcc acggtggagc aaacgaggtt gacaaggcag
     4321 ttgcccatga agggatggaa ccatatccat caaggaactt cgaagcaaac tgcggggctt
     4381 gtcacagcaa tgtaaccgat atttatgcaa catccattca ctacaatcta cacggaatgg
     4441 aaaatggctt ggcggcattt gcagatgtta tgtccttgtc tgagtctccc catcacgagg
     4501 aggtatttga ccaggattgc ttcaagtgtc atgcaacctg tggagactgt catgtaagca
     4561 gggcaaagaa cttttcaaat ggtcttgttg accagcacac tttcctggga actccatcaa
     4621 tggaggaaaa ctgctacgct tgccataatg caaggaatgc aggggagtac atgggtctga
     4681 taggatttgg aggagatgtc cactatgaag caggcttgac atgtatggac tgccacccgg
     4741 ttgacaactt ccacggatct gaaacagcaa caaaaaatat gtgggaggag aaccttcccg
     4801 catgtacgga ttgtcacacg gacaaggatc cggcagttgc aacggacgta aaccacaagg
     4861 ttcatgggga ttcactgtca tgtcaggtat gtcatgctca ggcaaacaac aactgctttg
     4921 agtgccatct ggatccaaag gaggatggga gtggacttca aagctcatct gagagcaaga
     4981 ttatgttcag agtgggactt aaccccaatc caacggagga aaggccttac aaatacgttg
     5041 ccttgaggca tgttccagga caggaagcca tgcttgatgt ggttggggag aacatgatgc
     5101 ctaacttcaa tgaaataaca aactggaagt attcgcctac ccataatata caggcaagta
     5161 ccttccaaaa tgaaagctgt gaatcctgtc atgaaaatga tagaatatgg ttaagtgaag
     5221 atgatttcag agagacagac agtgaagcca acttcagact tataccagaa agaccgcctt
     5281 cattggacca ctaagaaata atgggaaaag gggagcttgc tccccttttt cttaaatcaa
     5341 aacaagggga ggtagatgac aatagataat ttcatccgta gattgaccag tctcggactg
     5401 gcaataacac tgtttatctt catagcagcc tactcaataa tagggaccgt tctacctcag
     5461 gggctacaaa gggaattcta tatggaaagc tatccctcaa tgggaagcat aatcacaagt
     5521 cttcaatttg accaggtata cagctcctgg atttttcgaa tactcttgtt cctcttcata
     5581 gttaacctgt caggatgtac cctaaagata ctgccaggcc agctgaaaaa aatgaataag
     5641 gaccatttcc ccaaaccaaa accggatggg gataatttgc atgcggaaaa catggatatc
     5701 cagaaattca ggaaaatcct ggagaagaag cgctatgcaa taacggagga tgaaaagggc
     5761 ttcaaggcaa gcaaacacag gattgggaat attggttctt
//
LOCUS       DASRQF010000127         4994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_422087, whole genome shotgun sequence.
ACCESSION   DASRQF010000127 DASRQF010000000
VERSION     DASRQF010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422087"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VFD79_03335"
     CDS             <1..695
                     /locus_tag="VFD79_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307483.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HZH93111.1"
                     /translation="FQNFSNTYMDLEDFKSYVNQALQEDIVGISISTRPDCINSKYLQ
                     FLSDIQYNKGIDITIELGLQTVNYHSLRKVNRGHGLAEFIDAVINIRTHGLRSCAHVI
                     LNLPWDDMADVIETARVISALGVNEIKLHSLYLMENTILADQYEKGEFKLHSREEYIR
                     RAITFLEYLDPEIVVQRIIGRAPEEGAIFVNWQESWWKIRDEIVHRMKHENTFQGSKF
                     NYLGGKALGIFN"
     gene            745..1167
                     /locus_tag="VFD79_03340"
     CDS             745..1167
                     /locus_tag="VFD79_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93112.1"
                     /translation="MVKFILGEKGSGKTKWLIDNANKDMAEGNGNIAFVDVDDNHIFS
                     LNYNVRLINAMEFNVLNVRSFYGFLCGIMAMDYDLEKIYVDGIYKVMKVEFNDMVYLI
                     DSLKRISEKYEVEFYINIDYTMAEVPENLRPYCLEIEV"
     gene            1218..1937
                     /locus_tag="VFD79_03345"
     CDS             1218..1937
                     /locus_tag="VFD79_03345"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZH93113.1"
                     /translation="MRKERLDKVLSNMGVGSRKEVKEMVKAGMVTVNGKVMKASDFKV
                     DVEGDEIVCGGKTIRYREFIYLQMNKPKDLVSSTDDPRDSTVIELLDDFHRNFRPFPV
                     GRLDKDTEGLLLITNDGKLAHDLLSPKKKVDKTYHAIIEGRLTDEHVEEFSRGILLDD
                     GYHTLPAQLNIISSGPVSAAEITIQEGKYHQVKRMVAALGMRVSYLKRIRMGPLYLDE
                     SLAPGQYRELTEEEIRLLLDR"
     gene            1984..2544
                     /locus_tag="VFD79_03350"
     CDS             1984..2544
                     /locus_tag="VFD79_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6648 family protein"
                     /protein_id="HZH93114.1"
                     /translation="MYNLIYHRKKGLFATFFEHRSSLITQYENGDITKREFLEYNYDF
                     VRRMNVKPFLKVDSFEKGMYNYQYYNVLAKYFRMLAKEVKASGKHQKYYVQYLNKGNN
                     YYHEKDKSALALLRLIEFEGVEAYFIKCESKGLKDKLYEILLLDYKEAIFHSKAIWLL
                     DILKAEGVFSEKRRVSVIDEYINETY"
     gene            complement(2576..3088)
                     /locus_tag="VFD79_03355"
     CDS             complement(2576..3088)
                     /locus_tag="VFD79_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93115.1"
                     /translation="MKFVDKARDVLYDSVDYIFMIVIVLLVVGVIGWRLDVLFAKDAL
                     ESHPSGQVIVDNTGRGEDEPNQPPDELEVIVEGEEEPGYQEEPAQEPQNQPEQPAQPS
                     ATIVQVEIPVGSMPGKIGLILEEQGLVTSSREFIAKAVELGLDTKLKSGTYNIQLGLP
                     TEDVVRLLTN"
     gene            3191..4171
                     /locus_tag="VFD79_03360"
     CDS             3191..4171
                     /locus_tag="VFD79_03360"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZH93116.1"
                     /translation="MRELRIEKNESQQRLDRFLKKYLADATQGFIYKMIRKKNIKVND
                     ERAKPDMMLEEGDLVQLYLAEETIEKFKGERQHKRSRIDLDIVYEDENIVLMNKPVGL
                     LSHGTGEEFQDNVVDALVGYLIDNGDYVPRLEKTFTPSICNRLDRNTSGIIIGAKNYQ
                     SLQLVNQAIKEGHVGKYYMTIVKGRVDKDFEHKAFLTKDEERNRVEISDDDSEGGKEI
                     WTRFKVLKREGDYTLLEVELITGRTHQIRSHLASLGYPVIGDRKYGNRRINEDFKSKY
                     GLTDQWLHGYRLVLQGMEEPLDYLNTREFTAPAGGLFARIEKDLFGQRGD"
     gene            4173..>4994
                     /locus_tag="VFD79_03365"
     CDS             4173..>4994
                     /locus_tag="VFD79_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HZH93117.1"
                     /translation="MGKQISLAVEGYGELQMPEGTTLLDVSKKVHGSDHMRYLGARVN
                     NEIYPLYKDAEDGFQIQFLDNRDVDGYRIYTKTISAVFIMACKELYPDKDAIIHHFLG
                     PGLYAELDDRHGISFRETEILQEKMEEIIKRDIPIKRSDYSREEALEIFREHDYSDKV
                     RLISSMDKDTVSIYRIGDHTDSFHGYLAPSTGFVDEFKLKYYYPGVIILFPSRKNHYN
                     MDNFRDQKKLAKIFNESNNWLEILDLANIGSLNERINNGHLRDLILISEALHEKRI"
BASE COUNT         1550 a          952 c         1242 g         1250 t
ORIGIN      
        1 attttcagaa tttttccaac acatatatgg acctggagga ttttaaatcc tatgtaaatc
       61 aggcgcttca ggaagatata gttggaattt caatatctac aaggcctgac tgtatcaatt
      121 cgaaatattt gcaattttta agtgatatcc agtataataa gggtatagat ataacaatcg
      181 agttgggact acagaccgtc aattatcata gtctcaggaa ggtaaacagg ggccacggac
      241 tggctgagtt tatagatgca gtgatcaaca tcaggaccca tggcctcagg tcatgtgctc
      301 acgtgatcct gaatctgcct tgggacgaca tggcggatgt cattgagaca gcgagggtga
      361 tatcagctct cggtgttaat gagataaagc ttcacagtct ctacttgatg gagaacacca
      421 tactggcaga ccagtatgaa aaaggtgaat tcaagcttca ttccagagaa gagtacatca
      481 gaagagcaat aacatttttg gagtatctag atcccgagat cgtagtccaa agaatcatcg
      541 ggagggctcc agaggaaggc gcgatttttg tcaactggca ggagtcatgg tggaagatca
      601 gggatgaaat agttcaccgc atgaaacatg aaaatacttt ccaaggcagc aaattcaatt
      661 accttggagg taaagcctta ggaatcttta attaatcatc tgaagtagga taatattaag
      721 acaagaaagg taaggtggtt tatcatggtt aaattcattt taggtgagaa ggggtcaggg
      781 aagacaaagt ggttaatcga caacgccaac aaggatatgg cagaaggcaa tggcaatatt
      841 gcttttgttg atgtggacga taatcacata ttcagcttga attataatgt tagattaatt
      901 aatgccatgg agttcaacgt gcttaatgtg cggtccttct atggcttttt atgtgggata
      961 atggcaatgg attacgatct ggaaaaaatc tatgtggacg gaatctacaa ggtcatgaag
     1021 gttgagttca atgacatggt ttacttaatt gactctctga agaggatatc tgaaaagtat
     1081 gaggttgagt tctatatcaa catcgattac acaatggctg aggtacctga aaatcttaga
     1141 ccatactgcc tggaaataga agtttaggga gggagccttc gggctccttt agttttttag
     1201 aaaagggggg tgaagctgtg agaaaggaga gacttgacaa ggttctatcc aacatggggg
     1261 tgggatccag gaaggaagtc aaggagatgg taaaggccgg aatggtcaca gtaaatggaa
     1321 aggttatgaa ggcatctgac ttcaaggtgg atgttgaggg ggatgagata gtttgcggtg
     1381 gcaaaacaat cagatacagg gagttcatat atcttcagat gaacaagccc aaggatttgg
     1441 tctcctcaac tgacgatccc agagactcga ctgttattga gcttctggat gatttccata
     1501 gaaatttcag gccatttcca gttggaagac tggacaagga tactgaaggc ctcctgctca
     1561 taaccaacga cggaaagctt gctcacgacc tcctatcccc taagaaaaag gtggacaaga
     1621 cctaccatgc aataatagag ggaaggctta ctgatgaaca tgtggaggaa tttagcaggg
     1681 gaattctcct ggatgatggt taccatacac ttccagccca gcttaatata atttcttccg
     1741 ggcctgtttc cgcagccgaa ataacaattc aggagggcaa gtaccatcag gttaaaagga
     1801 tggtggcagc ccttggaatg agggtttcat accttaagag gatcagaatg ggtcccctct
     1861 atttggacga gtctcttgcg ccaggacaat acagggaatt gactgaagag gaaattagat
     1921 tgttgctgga caggtgattt atggtataat agccactagg aacaaagtat aatattagaa
     1981 tgggtgtata atttgattta tcacagaaaa aaaggattgt ttgcaacctt ctttgaacac
     2041 aggtcttcgc tgattacaca gtatgaaaat ggagacataa ccaagaggga gtttctggag
     2101 tacaactacg atttcgtaag aaggatgaac gtaaagccct tccttaaggt ggacagcttt
     2161 gaaaagggta tgtataacta ccagtactac aatgtcctgg ccaagtactt caggatgctg
     2221 gccaaggagg tcaaggcttc cggcaagcac caaaaatact acgtacagta cctgaacaag
     2281 ggaaacaact attaccatga aaaggacaag tctgcccttg ccttgcttag gctcattgaa
     2341 tttgaagggg tggaggcata ctttatcaag tgtgaatcca agggtttaaa ggacaagctt
     2401 tacgagatac tactccttga ttacaaggag gccatcttcc actcaaaggc catctggcta
     2461 cttgatattc taaaggctga aggtgtcttt tccgagaaga gaagggtgtc ggtaatagac
     2521 gagtatataa acgagacgta ctaataaaga aaagactggc tgaatagcca gtcttttaat
     2581 ttgtaagaag tcttactaca tcttcagttg gtagtccaag ctggatatta taggtaccgg
     2641 acttaagctt ggtatccaga cccagttcaa cagccttggc aatgaattcc ctgctgctgg
     2701 tcacaagccc ctgctcttcc agtataagcc caattttacc gggcatgctc cccacgggga
     2761 tttcaacctg tacaattgtg gctgatggct gtgcaggttg ctcaggctga ttctgtggct
     2821 cctgtgcggg ttcctcctgg tatccaggct cttcctcacc ttccactatg acctcaagct
     2881 catccggtgg ctggtttggt tcatcttctc ccctcccagt gttgtcgacg atcacctgtc
     2941 cggaagggtg agattccaat gcatccttcg caaaaagaac gtcaagtctc cagccaatca
     3001 ctcccactac aagaagaact atgacgatca taaatatata gtcaaccgag tcgtacaata
     3061 catcccttgc cttatccaca aacttcattc aagttcacct ctatgtcctc taaaacgcct
     3121 gtttctttcc tttttcactc atattgtagt ataattataa cataaaagag aggaaaaggt
     3181 gagataaact ttgagagaac taaggataga gaaaaatgag agtcagcaaa ggcttgacag
     3241 atttttaaag aaatacctgg cagatgcgac acagggcttc atctacaaaa tgatcagaaa
     3301 gaagaacata aaggtcaacg acgaaagggc caagccggac atgatgctgg aggagggaga
     3361 ccttgtacag ctgtacctgg cagaggaaac catagagaaa ttcaagggtg aaaggcagca
     3421 caaaaggtcc cgaatcgacc tggacatagt atatgaggat gaaaacattg tgcttatgaa
     3481 caagcctgtg gggcttcttt cccatggaac cggcgaggaa ttccaggata atgtagtgga
     3541 tgccctggtt ggctatctta tagacaatgg agactatgtt ccaaggctgg agaagacctt
     3601 tacgccttca atctgcaaca ggctggacag gaacacctcc gggataatca ttggtgcaaa
     3661 gaattaccag agtcttcagc ttgtcaacca ggccatcaag gagggacatg tgggcaagta
     3721 ctacatgaca atcgtcaagg gtagggtgga caaggatttt gagcacaagg catttctcac
     3781 aaaggatgag gaaaggaaca gggttgagat aagtgacgat gattcagaag gtggcaagga
     3841 aatctggacc aggttcaagg ttctcaaaag ggagggtgac tacacactcc tagaggtgga
     3901 gcttataaca ggaaggaccc accagatcag gagtcatctg gcatccctgg gatatcccgt
     3961 aataggtgac agaaagtatg gaaacagaag gataaatgag gatttcaaaa gcaaatacgg
     4021 tctgacagac cagtggcttc acggctacag gctggtactt cagggtatgg aagagcctct
     4081 tgattacctt aacaccaggg agtttacagc ccctgcaggt ggattgttcg cacgtataga
     4141 aaaggatttg tttggtcagc gaggtgattg atttgggaaa gcagataagt ttggcagttg
     4201 agggttatgg agaactacag atgccggagg ggacaaccct tttggatgtt tcaaaaaagg
     4261 tgcatgggtc tgaccacatg aggtatctgg gtgcaagggt caataatgag atctaccccc
     4321 tatacaagga tgctgaggat ggtttccaga ttcagttttt agataacagg gatgtggatg
     4381 gatacaggat atatacaaag accatttcag cggtcttcat aatggcctgc aaagaactgt
     4441 atccggacaa ggatgccata atccatcatt tcctggggcc tggactctat gcggagctag
     4501 atgacaggca tgggatttcc tttagggaga cagagattct ccaggaaaag atggaagaga
     4561 taatcaagag ggatattccc atcaagaggt cggactattc cagggaagag gcacttgaaa
     4621 tattcaggga acatgattat tcagacaagg taaggcttat ttcatccatg gacaaggata
     4681 cggtgagcat atacaggatt ggggaccaca cagattcctt ccatggttat cttgcaccct
     4741 caacgggttt tgtggacgag ttcaagctga aatattacta ccctggagtg atcatactat
     4801 tcccaagcag aaagaaccat tacaacatgg ataatttcag ggaccagaag aagcttgcaa
     4861 aaattttcaa cgagtcaaac aactggcttg aaatacttga cctggccaac atcggttcac
     4921 tgaatgaaag aataaacaat ggacatctcc gggaccttat tctaatatcc gaggcacttc
     4981 acgaaaagcg gata
//
LOCUS       DASRQF010000128         2455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1106924, whole genome shotgun sequence.
ACCESSION   DASRQF010000128 DASRQF010000000
VERSION     DASRQF010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106924"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="VFD79_03370"
     CDS             <1..1244
                     /locus_tag="VFD79_03370"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HZH93118.1"
                     /translation="MNHENIMYSWLLNFQYLTQEYMRMYKESKKYPEGDIYVFSDPDY
                     RPPSHPDGLALFDPEHNTALLLGMRYFGEHKKGTLTLAWGLANRNGFASCHGGMKRYN
                     FKDGNKFTMGVFGLSGSGKSTLTHEKHDGKYDITILHDDAYIISTDEGYSIALEPSYF
                     DKTQDYPPAHPANKYLLTVQNCGVTLDEDGNKVIVTEDIRNGNGRAVKSKLWAENRVN
                     KIDEPVDAIAWLMKDATLPPVIKINNSVLASVMGATLATKRSTAERLAKNVDMNALVI
                     EPYANPFRTYPLNDDYEKFKKLFEERDVDCYIFNTGHFLDKKIPKEVTLNLLEGIATG
                     SIQFKQLGSFEDLQTVDVEGFVPAFDEDYISMWQNSIAYRKEFIKHMTNFKSGRDKLP
                     DEALLELEKLEEQIESMKTVG"
     gene            1327..2163
                     /locus_tag="VFD79_03375"
     CDS             1327..2163
                     /locus_tag="VFD79_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HZH93119.1"
                     /translation="MIKLGKRQDLKIKRFASVGAYLNDDEDNGDDVLLPKSQVPEGAQ
                     VGDEVNVMVYRDSKDRIIATVNRSLAEVGEIKKLMVISSSKIGYFMDWGLEKDLFLPF
                     SETVGAVEKFKSYLVALYIDKSDRIAATMKIKDYLSTDSPYKENDKAVGTIYSVNRDI
                     GAFVAVDNKYDGLIQKKNLYGIYEMGDEIEVRVNMVHDDGKLDLSLRERGHLQMDEDA
                     EVILKAMKASGGSLPLNDSSPPHEIKKTLKMSKSGFKRAVGKLYKEGIIQITSKGINL
                     KK"
     gene            2173..>2455
                     /locus_tag="VFD79_03380"
     CDS             2173..>2455
                     /locus_tag="VFD79_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93120.1"
                     /translation="MASITVNFNTIEMMIYFWESVADRDKVSDKYLVELAGHDDLAPI
                     YDEEFSKDSFRKVLSAISNRELLNHPTKKESRFWNLNMWMLEDMDNMREM"
BASE COUNT          798 a          472 c          621 g          564 t
ORIGIN      
        1 cgatgaatca cgagaacata atgtattcat ggctactgaa cttccagtat ctgacccagg
       61 aatacatgag gatgtacaag gaatccaaga agtatcctga gggtgacata tatgtattct
      121 cagatcctga ctacaggcca ccctcacatc cagacgggct tgcccttttt gaccctgaac
      181 acaacaccgc cctactcttg ggaatgagat actttggaga gcataaaaag ggtacgttga
      241 cgctggcatg gggtcttgcc aacagaaatg gatttgcatc ctgccacggt ggaatgaaaa
      301 gatataactt caaggatgga aacaagttta caatgggagt attcggactt tcagggtcag
      361 gaaaatcaac ccttacccat gagaagcacg atggaaaata cgatataaca atactacacg
      421 acgatgcata cataatatca acagatgaag gctactccat agccctggag ccatcatact
      481 ttgacaagac ccaggactac cctcctgcac acccagccaa caagtacctg cttacagtcc
      541 agaactgtgg agttacactg gatgaggatg gcaacaaggt cattgtaact gaggatataa
      601 gaaatggtaa cggaagggct gtcaagtcaa agctatgggc agaaaacagg gtcaacaaga
      661 ttgatgagcc tgttgatgcg atagcatggc tgatgaagga tgcaaccctg ccaccggtta
      721 taaagataaa taattccgtg ttggcatcgg tcatgggagc gaccctggca acaaagaggt
      781 caacggcgga aagacttgca aagaatgtcg atatgaatgc ccttgtaatt gagccttatg
      841 ccaacccgtt cagaacctac cccttgaatg acgactatga gaagttcaag aagttgtttg
      901 aggagaggga tgttgactgc tatatattca acacaggaca tttcctggac aagaagattc
      961 caaaggaagt caccttgaat cttctggaag gtattgcaac cggcagtatc cagttcaagc
     1021 agctgggaag ctttgaagac cttcagactg tagatgtgga gggctttgta cctgccttcg
     1081 acgaggacta catctcaatg tggcagaatt caatagcata caggaaggaa ttcataaagc
     1141 atatgaccaa cttcaagagt ggtagggaca agcttcctga tgaagccctt cttgagcttg
     1201 agaagctgga ggaacagata gaaagcatga aaacagtggg ttaacccgct gtttttattt
     1261 taaaggatat ttaataatat tcaatcatgt gatatgatag ggttaataaa gaaaagtagg
     1321 tgtactatga ttaaactagg taaaagacaa gatttaaaga ttaaaagatt tgcttcagta
     1381 ggtgcttatc ttaacgatga tgaggacaat ggagacgatg tacttcttcc aaaatcacag
     1441 gttccagagg gagcccaggt tggggatgaa gtgaatgtaa tggtttacag ggacagcaag
     1501 gacaggataa ttgccacagt aaaccgatca ctggcagagg ttggtgagat caagaagctt
     1561 atggttatca gctcctcaaa gatcggatac tttatggact ggggactgga aaaggatctt
     1621 ttcctgcctt tcagcgagac cgttggagct gttgagaagt tcaaaagcta tcttgtggcc
     1681 ctatatatag acaaaagtga caggatagca gcgacgatga aaatcaagga ttatcttagc
     1741 acagattcgc cctacaagga gaatgacaag gctgtgggaa caatctacag cgtaaacagg
     1801 gacattggtg catttgttgc agttgacaac aaatacgacg gccttatcca gaagaagaac
     1861 ctctatggaa tctacgaaat gggggatgag attgaggtaa gggtcaacat ggtccatgat
     1921 gacgggaagc tggacctgag cctgagggaa agaggacacc ttcagatgga tgaggatgca
     1981 gaggttatct tgaaggccat gaaggccagt ggaggatctc ttccccttaa tgacagcagc
     2041 ccgccacatg aaatcaagaa gacacttaaa atgagcaagt ctggtttcaa gagggcagta
     2101 ggaaagctct acaaggaagg cattattcag ataacatcaa agggaatcaa tctcaagaaa
     2161 taggaggatg aaatggcgag tataacggtt aacttcaata ctattgaaat gatgatatac
     2221 ttctgggaat cggttgctga cagggacaag gtgagcgaca agtaccttgt tgagcttgca
     2281 ggccatgatg acctggcacc catctacgat gaggagtttt ccaaggattc attcagaaag
     2341 gtcctttcgg caatctccaa cagggagctg ctgaaccatc ccacaaagaa ggaaagccga
     2401 ttctggaacc taaacatgtg gatgcttgag gatatggaca acatgaggga aatgg
//
LOCUS       DASRQF010000129         6332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_759253, whole genome shotgun sequence.
ACCESSION   DASRQF010000129 DASRQF010000000
VERSION     DASRQF010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6332
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759253"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VFD79_03385"
     CDS             <1..317
                     /locus_tag="VFD79_03385"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HZH93121.1"
                     /translation="PLVLHGASTVLPEFVELCNKYGGDIPGAQGVPEEMLRRAAQMNV
                     AKINIDTDLRLAMTAAIRQFMVENPSEFDPRKYLGPARAAIKGMVQHKIRNVLGSSGS
                     NL"
     gene            365..739
                     /locus_tag="VFD79_03390"
     CDS             365..739
                     /locus_tag="VFD79_03390"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HZH93122.1"
                     /translation="MDSFPDIERVHQILDEIAETLPLEFFNRLNEGVVLLPQHKYHPR
                     SRSDRRLYVMGEYHKSFTGSHIRIYYGSFKRVFRNISEEELRKHLEHTLLHEFTHHLE
                     TLAGERGLIIKDQEALRKYLQE"
     gene            complement(791..1549)
                     /locus_tag="VFD79_03395"
     CDS             complement(791..1549)
                     /locus_tag="VFD79_03395"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HZH93123.1"
                     /translation="MKKKLDEGKNVLGTFFELGSEEVVESLGYTGLDFIIVDTEHGPY
                     SVESTARFTRAAEVVGLTTMVRVSDISRPSILRNLDIGAKGLIVPCVETVDEVRKLVE
                     WAKYTPVGKRGYFKARSAGYGEKDFARNLDEYFATCNRETLLIPQCETIGCLESIEDI
                     MAMDGVDGIFVGPFDLSIGLGIPAQFDDPSFKAALGRILKAAKDNNKYAFIFSPDSNS
                     AKSMFEMGYQGVAVNMDAGFLMGAVKSAVKEIME"
     gene            1684..2505
                     /locus_tag="VFD79_03400"
     CDS             1684..2505
                     /locus_tag="VFD79_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZH93124.1"
                     /translation="MIVKTLLENRSVKERYETEHGLSLYIEAGERKILFDAGETDAFI
                     RNAKRMGVDLAEVDMMILSHGHYDHGGGIKYFMDLNKKALIYIHKEAFRPHASIKEGE
                     RKDAGIDDSLLHNNRVRLVDEDLYFGDELVLFSHIKGKEMVPRGNSRLLMKKDSGWIK
                     DNFHHEQNLLIKEGNKRVLVVGCSHRGIVNILDQCEKHLDRPLTAVLGGFHLYDLDKN
                     KEEDLSFLKGIAGRLEENNASYYTGHCTGYDQFSLLKEMMGNRIHYLATGSIVEI"
     gene            2661..3644
                     /locus_tag="VFD79_03405"
     CDS             2661..3644
                     /locus_tag="VFD79_03405"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HZH93125.1"
                     /translation="MAREREKIYGCLLGGAVGDALGYPVEFMSYTEILDAYGEKGIDS
                     LERGKAFISDDTQMTLFTAEGILLTSSKDKVVDNIYLAYLRWLSTQGYNIPVVEEGWL
                     IHDKRFHVRRAPGNTCLSSLQSGKMGTIHTPINQSKGCGGVMRVAPLGILCPPDDAFA
                     YGMETAAITHGHPSGYYSAGALARLVSLLLSGEDLVRAVEDTIRHLEGYEDSEETLHI
                     LQKAFRLSQEDIEPLQAMKSLGEGWVGEEALAISVYCSLRFKDDFKSALIASVNHDGD
                     SDSTGAITGNIMGAALGSESIPEQWGHRVELNDKINMMAERLHYKHELYYI"
     gene            3865..>6332
                     /locus_tag="VFD79_03410"
     CDS             3865..>6332
                     /locus_tag="VFD79_03410"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004821166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HZH93126.1"
                     /translation="MLHEIKESLKIDGVERIRILNRYDFTCRNEESFEETVNKILSEP
                     NVDLVHKTELPEFQGRALAVEYLPGQYDQRADSAEQCIQILLGDMEAKVKSAKVFLMV
                     GNISDENFRKIKDYLINPIDSRQASMELPITLEDRTVEPEDVEIHNGFIDYSHEDLEK
                     FRIEMGFAMNFDDIILIQDYFRNDEKRDPSVTELKVIDTYWSDHCRHTTFFTEIQNIK
                     IQEGKYQQVFQDALDRYHKSREKTGVRKPVSLMDLATINARETRQDGMLENLDISDEI
                     NACSIEIDVDVDGNIEKWLLMFKNETHNHPTEIEPFGGAATCLGGAIRDPLSGRSYVY
                     QAMRVTGSGDPRTPMEETIPGKLPQRKITKEAAKGYSSYGNQIGLATGQVTEIYHQGY
                     IAKRMEVGAVIAAAPKSNVIRMAPKPGDIVLLVGGKTGRDGIGGATGSSKSHTEESLE
                     TSGAEVQKGNAPEERKIQRLFRKPELARLIKKCNDFGAGGVSVAIGELADSITIDLDV
                     IPKKYEGLDGTELAISESQERMAVVIDPKDLEVFKELGRQENLEVTKVADITDTGRLI
                     MNWRGKSILDIKRDFLDTNGARQFADVILGSPLDKEESAKAFPTFKEGMERTLSDLNV
                     CSQKGMNAMFDNTVGAATVLMPEGGKHRLTPIEGMAAKIPVLQGDTTTASLMTFGFDP
                     EICQWSPYHGGVYSVIEAISKLVAMGGDYRKARLSMQEYFEKLENDPLKWGKPFSALL
                     GAYTAQDELGIAAIGGKDSMSGTFKDISVPPTIITFAVATESTDKILSPEFKKTDSQV
                     YLAEIKPDENNLPDFQMVREAYEE"
BASE COUNT         2005 a         1230 c         1530 g         1567 t
ORIGIN      
        1 cacctttggt acttcacggt gcatcaactg tacttccaga gtttgtggag ctctgcaaca
       61 agtatggcgg ggacattcct ggagctcagg gagtacctga agagatgcta agaagagctg
      121 ctcagatgaa tgttgcaaaa ataaatatag atacggacct gagacttgcc atgacagctg
      181 caataagaca attcatggtg gaaaatccat cggaattcga cccaagaaaa tatcttggac
      241 ctgccagagc agccataaag ggaatggttc agcacaaaat caggaacgta ttgggatcaa
      301 gtggatcaaa tctttaagat aaaggggcat attgtccctt tgattttttg taggagttgg
      361 tattatggac agttttccag atattgaaag ggttcaccag atactggatg agattgcaga
      421 aaccctgcct cttgagtttt tcaacaggct gaatgagggt gttgttcttc tgccccagca
      481 caaataccac ccaagaagtc gtagtgacag aaggctctat gtaatggggg aataccacaa
      541 gagctttact ggaagccata taagaatata ttatggttca ttcaaaaggg tttttagaaa
      601 tatttccgag gaagaactta ggaaacacct tgagcacacc ctactgcatg aattcaccca
      661 ccacctggaa acacttgcag gtgaaagagg actgataata aaagaccagg aagcactgag
      721 aaaatatctt caggaatgat ccaaattaaa aaggatgaaa ccgtcaaaat ggtttcatcc
      781 tttctttgta ctactccatt atttccttta ctgcagactt gactgcaccc atgagaaaac
      841 ctgcatccat gttaactgca acgccctgat aacccatctc aaacatgctt ttggctgagt
      901 ttgaatcagg agaaaagata aaggcatact tgttgttgtc cttggctgcc tttagaatcc
      961 taccaagagc agccttgaaa cttggatcat caaactgggc agggataccc agaccgatgg
     1021 aaaggtcaaa aggcccaaca aaaatcccat ctaccccatc cattgccatt atatcttcaa
     1081 tactttctag gcaaccgatt gtctcacatt ggggtataag tagtgtttcc ctgttacagg
     1141 tggcaaaata ctcatcaaga ttccttgcaa aatccttttc tccgtagcct gctgacctgg
     1201 ccttgaagta gcccctctta cctactgggg tgtacttggc ccattcgaca agcttcctga
     1261 cctcatcaac tgtctccaca caagggacga taagtccctt tgcaccaata tcaaggttcc
     1321 ttaagatcga aggcctggaa atatcactga cccttaccat ggtggtcaag ccaacaacct
     1381 cagcggccct tgtgaatctt gcagtactct caacactgta tggtccgtgc tctgtatcaa
     1441 caattataaa gtccagaccc gtatatccaa gggattccac aacctcttcg cttccaagtt
     1501 caaaaaaagt gccaagaaca ttcttgccct catcaagctt tttcttcaca ttatttaaca
     1561 aaaataccac cgtcctttca tataattact ttactgttat ctagtgtaaa agtcaatata
     1621 tagaagacta atgaaactaa ctggtgattt tgggtaaatg tttgtatgaa gggagtgagg
     1681 aaattgatag taaagacttt gcttgaaaat cgatctgtaa aagagagata tgaaacagag
     1741 catggactca gtttgtacat tgaagcagga gaaagaaaaa tactgtttga tgccggtgaa
     1801 actgatgcct tcatcaggaa tgccaagcgg atgggggtag acctggctga agtggatatg
     1861 atgattcttt cccatggcca ttacgaccat ggaggaggga taaaatactt tatggacctt
     1921 aacaagaagg ctctcatata tatacacaag gaggctttca gaccacacgc ctcaataaag
     1981 gaaggagaga ggaaggatgc gggaatagac gatagccttc tccataataa cagggtaaga
     2041 ttggtggatg aggatctgta ttttggggat gaattggttt tgttttccca tatcaaggga
     2101 aaggagatgg tccctcgagg aaactccaga ctgttgatga aaaaggattc gggatggata
     2161 aaggataatt ttcaccatga gcagaatctt ttgatcaagg aagggaataa gagggttcta
     2221 gtggttggct gttcacaccg gggaatagta aacattcttg accaatgtga aaagcatctt
     2281 gacaggccat tgactgcagt cttaggtgga ttccatctgt acgacctgga taagaataaa
     2341 gaggaggacc tttccttcct aaaaggaata gcaggcagat tggaggaaaa taatgcatcc
     2401 tattacacag gtcattgtac aggctatgac caattttctc tgttaaagga gatgatgggg
     2461 aacaggattc actaccttgc aaccggttca attgttgaaa tatgaactga ttacaataag
     2521 ttgccataaa attcctttta gtcattgaaa ctgtgggatt tgatttattt aaaaaattat
     2581 aaataattag tgtaaaacaa ctaataatcg agtataattg aaatatagag tgacaaaata
     2641 tttggaagac gagggataat atggcaaggg aaagagaaaa gatatatgga tgtctccttg
     2701 gtggtgcggt tggagatgcc ttaggttacc ctgtagaatt tatgagctac actgagatcc
     2761 ttgatgccta tggtgaaaaa gggatagata gccttgagag gggcaaagcc tttatatcag
     2821 atgacaccca gatgacactt tttactgctg agggaatcct attgacctca tcaaaggaca
     2881 aggtagttga taacatatat ctggcctacc tgaggtggct atccacacag ggctataata
     2941 ttcccgtcgt agaagaaggc tggcttatcc atgacaaaag attccatgtt agacgagccc
     3001 cggggaatac ctgcctctca tcattgcaga gtggaaagat gggaacgata catactccca
     3061 taaaccagag caaggggtgt ggtggtgtca tgagggtcgc acccttgggt attttgtgtc
     3121 cacctgatga tgccttcgca tacggaatgg aaaccgctgc cataactcac gggcatccat
     3181 ctggatatta ttctgcaggt gctctcgcaa gattggtatc actactcctt tcaggtgagg
     3241 atcttgttcg ggcagttgag gatacaattc gccatcttga gggatacgaa gacagtgaag
     3301 aaacccttca tatcttgcag aaggccttta gactaagcca ggaggatatc gagccattgc
     3361 aggccatgaa gagcctgggt gaaggctggg tcggtgagga agctcttgca atttctgtat
     3421 actgttcact gcgattcaag gatgacttta agagcgcatt gatagcatca gttaatcatg
     3481 acggtgacag cgacagtact ggagcaataa caggaaacat tatgggtgca gctcttggtt
     3541 cagaaagcat cccagagcag tggggtcaca gggtagaact taatgataaa atcaatatga
     3601 tggcagaaag attgcactac aaacatgaat tgtattacat ttaattagct aatacctccc
     3661 aatataaatt tcccctgcaa ggatattcat tccttatgca ggggaaattg ttttcatttg
     3721 ttaaatttcc attatatact tgcctaaagc gtagggatat tctaagatga atatataaat
     3781 aaaataaagg cggtggttgt catttccgat attagaagag tttatgtaga aaaaagagaa
     3841 ggctttgatg tggaggccat gcatatgctt catgaaatca aggagtctct aaagattgat
     3901 ggagtggaaa ggataaggat tctaaacagg tatgatttta cttgtaggaa tgaggagagc
     3961 tttgaagaaa ccgtaaacaa gatcctttca gagcccaatg ttgatcttgt acataagacg
     4021 gaacttccgg aatttcaagg cagggccctg gctgttgaat acctgccagg ccagtacgac
     4081 caaagggcgg attctgctga gcagtgcatt cagattctcc ttggggatat ggaggcaaag
     4141 gtaaagtcag ccaaggtatt tttaatggtt ggaaacataa gtgatgagaa cttcaggaaa
     4201 atcaaggatt atctcattaa tcccatagat tcaagacagg catccatgga gcttcctata
     4261 accctggagg accgaactgt tgaacctgaa gatgtggaga ttcacaatgg attcattgat
     4321 tattcccatg aagaccttga gaagttcagg attgaaatgg gctttgccat gaactttgac
     4381 gacataatac ttatacagga ctactttaga aacgatgaaa agagagaccc atctgtcaca
     4441 gagcttaagg ttattgacac ctattggtca gaccactgtc gacacacgac cttcttcact
     4501 gagatacaga atatcaaaat tcaggaaggt aaataccagc aggtcttcca ggatgcattg
     4561 gacaggtacc acaaatcaag ggagaagaca ggagtaagaa agcccgttag ccttatggac
     4621 ctggcaacta tcaatgcaag ggaaacaagg caagatggta tgctggagaa cctggatatc
     4681 tcagatgaga taaatgcctg cagcattgaa atcgatgttg atgttgacgg aaatattgaa
     4741 aagtggctct tgatgttcaa gaacgagacc cacaaccatc caacggagat agagcccttt
     4801 ggtggtgctg ctacctgtct tggaggagca atcagagacc ctctttctgg gagaagctat
     4861 gtatatcagg caatgagggt gacaggaagt ggagacccaa ggaccccaat ggaagagaca
     4921 atccctggaa agcttccaca gaggaaaata accaaggaag cagccaaagg ctacagctcc
     4981 tatggaaacc agattggact tgccacaggg caggttactg aaatctatca ccagggatat
     5041 atagccaaaa ggatggaggt tggagcggta atagcagcag ccccaaaatc taatgtaatc
     5101 aggatggcac caaagccggg agacattgtc ctgctggttg gaggaaagac aggaagggat
     5161 ggcataggcg gtgcaaccgg atcatccaaa tcccacacag aggaatcatt ggaaacatct
     5221 ggagcagagg tacagaaggg aaatgcacct gaggagagaa aaatacagag actcttcaga
     5281 aaaccagagc tcgcgagact gataaagaaa tgcaacgact tcggagcagg tggagtatct
     5341 gttgcaatag gggagcttgc agatagcatt acaattgacc ttgatgtcat tcccaagaag
     5401 tatgaggggc ttgatgggac agagcttgca atatctgagt cccaggagag gatggctgtt
     5461 gtgatagacc ccaaggatct tgaagtattc aaggagcttg gtcgccagga gaaccttgaa
     5521 gtcacaaagg tggcggacat aacagatacg ggtagactga tcatgaactg gagagggaag
     5581 agtatactgg atatcaagag ggactttctc gacacaaacg gggccagaca gtttgcagat
     5641 gttattcttg gatctccatt ggacaaggaa gagtcagcaa aggccttccc gaccttcaag
     5701 gaaggcatgg aaaggaccct gtcagacctt aatgtatgct cccagaaggg tatgaacgca
     5761 atgtttgaca atacggttgg agctgcaacg gtactgatgc ctgagggagg aaaacataga
     5821 ttgaccccga ttgaaggtat ggctgcaaaa ataccagttc tccagggaga cacaacaaca
     5881 gcaagcctta tgacctttgg gtttgatcct gaaatatgcc agtggagtcc atatcacggc
     5941 ggagtttatt cggtaattga ggcaatttca aagctcgttg caatgggagg agactacagg
     6001 aaggcaaggc tcagcatgca ggagtacttt gagaagctgg aaaatgaccc gcttaagtgg
     6061 gggaaaccat tcagcgcact tcttggagcc tatacagcac aggatgagct gggaatagca
     6121 gcaataggag gaaaggattc catgtccgga acctttaagg atatttctgt ccctccaaca
     6181 ataatcacct ttgcagtggc aacagaaagt acagacaaga ttctttctcc tgagttcaag
     6241 aagacagact cacaggttta tcttgctgaa atcaagcctg atgaaaacaa ccttccagat
     6301 ttccaaatgg taagggaagc atacgaagaa at
//
LOCUS       DASRQF010000130         5664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_27561, whole genome shotgun sequence.
ACCESSION   DASRQF010000130 DASRQF010000000
VERSION     DASRQF010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5664
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27561"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1844
                     /locus_tag="VFD79_03415"
     CDS             <1..1844
                     /locus_tag="VFD79_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZH93127.1"
                     /translation="EYEKTLSDSNSDIKKLMARMYRHEKRMDFYKEYSELTYEADFKY
                     RESKIHVDQIPEIIEFIEGIEAELAHAMDERDKIDPKEDSRMKEDLKSFKEEAKEKRD
                     LEIKKVKERRKEGVISSKAETNAIKELKREYKNQVKLQEYRIPSMENKDFIRSKKKEL
                     KNGIKRSEKVLKADVADTRRKIPVEFDKTRPLVAYLTPLLPGLGQLINGQRRKALFFF
                     IASFFIYFIAIPYALGYGNYQGEGVAGLVSLAEGGARVHKSLIFMIEGIVAIFLILMA
                     IGLFYASFRDVLNTERKMIRGIRPSNWFETKRTIFQEGFPYVTSFPAMLVIIFIVLVP
                     VITTLLLSFTNMNPQNQSKFTWIGIENYKLIALGEGLAGSVFWLILVWTIIWTLVATT
                     LAILLGFFLALLVNNDRIKGKKFFRTIYILPWAVPAFITIMFFSIMVAPGGVITEILN
                     SIFNARIEIKNDTVLTRTALILIQGWLGSAYVFLLTTGVLQAIPGDLYEAAEIDGASS
                     FEKLRRITVPLVLFQIAPLLVTQYTFNFNNFSVIFLFNSGGPFDPQRYGNLAGSSDLL
                     ISYVYKLALVNQYQAIAAAITIFISLGVMFFAFLGFRRSKGFREGRL"
     gene            1844..2749
                     /locus_tag="VFD79_03420"
     CDS             1844..2749
                     /locus_tag="VFD79_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004837520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HZH93128.1"
                     /translation="MALFSRKKKKDKGLYLSDKPPLTVAGIIGLVMSYTILFAWVVAI
                     VWPLFQMVVSAFNGRQQQYIMGNENFEFSLRHFEFLYNETYYLTWVKNTLFIAISTAV
                     LTLIMVSFTGYAYSRYRFKGRKASLMAIMLIQTIPTFAGITAFFTMHSIVSSVIPAFS
                     RQMMLILIYAGPAIASNTFILKGYIDSVSTELDDAAKIDGCSNFDIYRLIIMPIARPM
                     LTIIALWSFIGPFMDYLLAKVLLTSPSTYTLAVGLFTLISDFRTMNQPAFAAGGLLTA
                     IPIVIMFIVLQKQLVSGLTSGSVKG"
     gene            2761..3849
                     /locus_tag="VFD79_03425"
     CDS             2761..3849
                     /locus_tag="VFD79_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012803604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZH93129.1"
                     /translation="MKVIMKNLGKKYEGRENFTLRNIDLEIDDQDFCVILGPSGCGKT
                     TLLRMIAGLNSITEGDLYFDERRVNTIPPKDRDIAMVFQSYALYPHMTVYDNMAFSLV
                     MRKERKKLIDERVKEAAELLQIEDYLYAKPADISGGQRQRVALGRAIVRKPSVFLMDE
                     PLSNLDAKLREHMRVELVRLHKSLGTTSIYVTHDQTEAMTMATKIILLDDGKIQQVGA
                     PEEFYNKPSNIFVARFIGSPTINIINGKYKDGKFVSETDTITLTPSKEDAEVLKEFEG
                     KNVSIGIRPERFESGGEFGDTFEATIDVIEMLGKEKTLFARLEDGSDLVISTPGHYDY
                     KEGETHTFGFDINAIHYFDGETGERINR"
     gene            3863..4891
                     /locus_tag="VFD79_03430"
     CDS             3863..4891
                     /locus_tag="VFD79_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZH93130.1"
                     /translation="MVKKRHRITIKDVAKEAGVSPSTVSRVISNNPRISRKTTEKVLT
                     HMTRLGYQPNAIARNLAKNSSRTIGLIMPDRPGEALLNPFFPEALRGIIKAATQASYD
                     ILLSSTPPGTDDYNTVRELVSTAKVDGVLLMTSKVENKSIQYLREMEFPFSVIGSPYE
                     ESDDSNHVDNDNEMASYELTTYLVKKGCKRLALITGGRFLTVTEERIKGFKRALKESG
                     LREEEGVIFVGEFSEETGKKYAREIIQSGREFDAVVATDDVVAYGVVSAFMEGGLKVP
                     EDIMVAGFNNSLLSRQCTIPITSVEIHADQLGANAVDVILENIHKGAPQKKRKVPHTI
                     YKRKSTFD"
     gene            5050..5664
                     /locus_tag="VFD79_03435"
     CDS             5050..5664
                     /locus_tag="VFD79_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93131.1"
                     /translation="MDHKLMEDNLEKIESVVSSKIVFGDDDNINEIHLVANGHRGPKQ
                     IARDVQSVLLATFDVDVDHKKISIAQIPDDTLKRRTSRVRLEGVLRESRDSRVTIKVT
                     VSLDGEEYTSSRTGVDSSRNSDRMLVETTLDVVQEASNFEDIFLFDDIRQVPVAGTNI
                     VVICVTGVIAGTEHRLCGASVVRNDYDKAVVKATLDAVNRFITK"
BASE COUNT         1799 a         1132 c         1336 g         1397 t
ORIGIN      
        1 atgagtacga aaagactctt tctgattcca acagtgacat taagaaactt atggccagaa
       61 tgtaccgcca tgagaagagg atggactttt acaaggaata cagtgagctt acctatgaag
      121 cagacttcaa gtatagggaa agcaaaatcc atgtggacca gattccggaa atcatagaat
      181 tcatagaagg aattgaggcc gaacttgccc atgcaatgga tgaaagggac aagattgacc
      241 cgaaagagga ttctcgcatg aaggaagacc tgaaatcctt caaggaggag gcaaaagaga
      301 agagagacct ggaaatcaag aaggtcaagg aaagaagaaa agaaggggta atttcaagca
      361 aggctgaaac caacgcaata aaggagctta aaagggaata caaaaaccag gtaaagctgc
      421 aggaatacag aataccttct atggaaaaca aggatttcat aagaagcaag aaaaaagagc
      481 tgaaaaatgg aattaaaaga agcgagaagg tactgaaggc agatgtggca gatacaagaa
      541 ggaaaatacc tgttgaattt gacaagacaa gacccctggt agcctacctg actcctctct
      601 tgcctggact aggtcagctt attaatggtc agaggcgaaa ggccctcttc ttctttattg
      661 catccttttt catttatttc attgccatac cctacgccct tggctatgga aactatcagg
      721 gtgaaggagt tgcaggcctt gtatctctgg cagagggagg agcaagggta cacaagtcac
      781 tgatatttat gatcgagggt atagtggcaa tattcctcat attgatggca ataggacttt
      841 tctatgcatc cttcagggat gttctgaaca cggaaagaaa aatgataagg ggaattaggc
      901 catcaaactg gtttgaaacc aaaaggacca tattccagga gggtttccca tatgtaacat
      961 catttccagc catgctggtg ataatattca tagtcctggt ccctgttata accacactcc
     1021 ttctttcctt tacaaacatg aacccgcaaa accagtccaa attcacctgg attggcattg
     1081 agaactacaa gttgattgcc ctgggcgagg gcttggcagg aagcgtgttc tggcttatcc
     1141 ttgtctggac cataatatgg acacttgttg caacaacctt ggcaatactg ttgggattct
     1201 ttctggcact cttggtcaac aatgatagga tcaagggaaa gaaattcttt aggaccatat
     1261 atatactgcc atgggctgtg cctgcattta taacaatcat gttcttcagc ataatggtgg
     1321 caccaggtgg agttataact gaaatactga attccatctt caatgccagg atagagatca
     1381 agaacgatac ggttctgacc agaacagcac tgatcctcat acagggctgg cttggaagtg
     1441 catatgtgtt ccttctaaca actggagttc ttcaggccat accaggagac ctttacgagg
     1501 cagccgaaat agatggagca tcatcatttg agaagctaag aaggataaca gtgcccctgg
     1561 tgctctttca gatagcaccc ctgttggtga cacagtatac atttaacttc aacaatttct
     1621 cggtaatatt tctctttaac agcggtggtc cattcgaccc ccaaaggtac ggaaacctgg
     1681 cagggtcatc agacctcttg atatcatatg tatacaagct tgccctggtg aaccaatacc
     1741 aggccattgc tgcagcaata acaatattca tttcacttgg agtgatgttc tttgcattcc
     1801 tgggcttcag gcggtcaaaa ggatttaggg aggggagatt ataatggcac ttttcagtag
     1861 gaaaaagaaa aaggacaagg gattgtatct ctctgacaaa cctccattga cagtggctgg
     1921 gataatcggt cttgtaatgt catacacaat cctctttgcc tgggtagtgg ctattgtctg
     1981 gcccttgttc cagatggtgg tatctgcatt caacggcagg cagcagcagt atataatggg
     2041 aaatgaaaac tttgagtttt ccctgaggca tttcgagttt ctctacaacg agacctacta
     2101 cctgacatgg gttaagaata ccctgttcat agcaatcagc acggcagtat tgacccttat
     2161 tatggtatcc tttactggat atgcctactc aagatacaga ttcaagggga gaaaggcctc
     2221 ccttatggcc ataatgttga ttcagaccat tccaacattt gctggaatca ctgcattctt
     2281 taccatgcat tcaatagtgt catcggtcat accagccttt agcaggcaga tgatgctcat
     2341 actgatatat gcaggtccgg ccatagcatc caacaccttt atactaaagg gatacatcga
     2401 ttcagtttcc acagagctgg acgatgctgc caagattgac ggctgttcaa acttcgatat
     2461 atacaggcta atcatcatgc caatagcaag acccatgctt acaataattg cactatggtc
     2521 atttataggt cccttcatgg actatctgct ggcaaaggtc ctacttacaa gtccgagcac
     2581 atataccttg gcagtgggat tgtttaccct cataagtgat ttcagaacca tgaaccagcc
     2641 ggcatttgcg gcaggtggtc tcttgactgc aattcccatt gtaataatgt tcattgtatt
     2701 gcagaagcag ctggtatctg gactgacaag tggatctgtt aaaggatagg agggaactgt
     2761 atgaaggtaa taatgaaaaa tctagggaaa aaatacgagg gaagggagaa cttcacctta
     2821 agaaatatag accttgaaat agacgaccag gatttctgtg taattcttgg tccatctgga
     2881 tgtggaaaaa cgaccctttt aaggatgata gcaggcctta actccataac agagggagac
     2941 ctttattttg atgaaagaag ggttaacacg attccaccaa aggacaggga catagccatg
     3001 gtattccagt cctatgccct ctacccccat atgaccgtct atgacaatat ggctttttcc
     3061 ctggtaatga gaaaggaaag aaaaaagctc atcgacgaaa gggtaaagga ggcagcagag
     3121 cttctccaaa ttgaagacta cctttatgca aagcctgcgg atatttccgg tgggcagaga
     3181 cagagggtgg ccctgggaag ggccattgtc agaaagccca gtgtctttct aatggacgag
     3241 cctctttcaa acctggatgc aaagctgagg gagcacatga gggttgaact ggtgagactt
     3301 cacaaaagtc ttggcacaac atccatatat gtaacccatg accagacaga agccatgacc
     3361 atggcaacaa aaatcatcct tttggatgat ggaaagatcc agcaggtagg agcacctgaa
     3421 gagttctaca ataagccttc aaatattttc gttgcaagat ttatcgggtc tccaaccata
     3481 aacatcatca atggaaaata caaggatgga aaatttgtct cggaaaccga cacaataacc
     3541 ttgacgcctt caaaggaaga tgctgaagtt ctgaaggaat ttgaaggaaa gaatgtctcc
     3601 attggaataa gacctgaaag atttgaaagc ggaggcgagt ttggggacac ctttgaagcg
     3661 acaatagacg taatagagat gctgggtaag gaaaagacct tgtttgcaag actagaggat
     3721 ggttcagacc ttgtgatttc aaccccgggc cattatgatt acaaggaggg ggaaacccac
     3781 acctttggat ttgatattaa tgcaattcat tactttgacg gcgaaaccgg agagagaata
     3841 aaccgctgat gggagtgtaa tcatggtcaa aaaaaggcat aggatcacaa taaaagatgt
     3901 cgccaaggag gcaggggttt ctccatcgac cgtttcaagg gtaatatcaa acaatcccag
     3961 aataagcaga aaaaccactg aaaaggtcct gacccacatg acaaggcttg gctaccaacc
     4021 aaatgcaatt gcaaggaacc tggcgaaaaa ctcctccaga acaatcggac tcataatgcc
     4081 agataggcct ggggaagcac tattgaatcc atttttccca gaggccctac gaggaataat
     4141 aaaggcggcc acccaggcat cctacgacat tctgctttca tcaacaccac ctgggaccga
     4201 cgactacaat acagtgcggg agttggtatc gaccgcaaag gtggatggag tcctgcttat
     4261 gaccagcaag gttgaaaaca agtcgatcca atacctaagg gaaatggagt ttcccttttc
     4321 tgtgatcggg tcaccctacg aggaatcaga cgattcaaac cacgttgaca atgataatga
     4381 aatggcatcc tatgagctga ccacatacct tgtgaagaag ggatgtaaaa gactggctct
     4441 aattacagga ggcaggtttc ttacagttac tgaggagaga atcaaaggct ttaagagggc
     4501 cctcaaggag agtggactta gggaagagga gggtgtcatt tttgttggtg agttcagtga
     4561 ggagaccggg aaaaagtatg ccagggaaat aatccagtca ggcagggaat tcgatgcggt
     4621 ggtggccacc gatgacgtgg ttgcctatgg agtggtttca gcatttatgg aaggtggact
     4681 gaaggttcct gaggacataa tggtagcagg atttaacaat agcctcctat ccagacaatg
     4741 caccattcca ataacctcag ttgagatcca tgcagaccag cttggagcca atgcagttga
     4801 tgtaattctg gagaatatcc ataagggagc accccagaag aagagaaaag tcccacatac
     4861 catatacaaa agaaaatcaa catttgacta aaatctgaca ttaatatgtc ggattttttc
     4921 aatattctgg aaattgccac tttattttga taaatcagct attttttaat tgaaagaccg
     4981 gtcaaatagt ggtaatatct tattatagtg gatcagtccg ctaaataatt ctgtattgcg
     5041 agggatatta tggaccacaa gctaatggag gataatttag aaaaaattga atccgtagtt
     5101 tcaagtaaaa ttgttttcgg agatgacgat aacatcaatg aaatacacct tgtagccaat
     5161 ggtcacagag ggcccaagca aattgcaaga gatgtgcagt cggttcttct tgcaaccttt
     5221 gatgttgatg tagaccacaa gaaaataagc atagcccaga tacccgacga tacactgaaa
     5281 agaagaacca gcagggttag gcttgaaggt gtacttaggg aatccaggga ttcaagagtt
     5341 acaatcaagg tgacggtatc ccttgatgga gaggaataca caagctctag aacaggtgtg
     5401 gactcctcca gaaattcaga caggatgctt gttgagacca ccctggatgt agttcaggaa
     5461 gcaagcaact ttgaagacat attcctcttt gacgatataa gacaggttcc tgttgcaggt
     5521 acaaacatag tagttatctg cgtaaccgga gtgattgcag ggacggaaca tagactttgt
     5581 ggtgcaagcg tagtaagaaa tgattatgac aaggcggttg tcaaggcaac tcttgatgcc
     5641 gtaaacagat ttatcacaaa atag
//
LOCUS       DASRQF010000131        16545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1738479, whole genome shotgun sequence.
ACCESSION   DASRQF010000131 DASRQF010000000
VERSION     DASRQF010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 16545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16545
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738479"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VFD79_03440"
     CDS             <1..558
                     /locus_tag="VFD79_03440"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HZH93132.1"
                     /translation="YDFERLAIPIRVFRLLGVETLVLTNAAGAINTDMVPGDIMLIND
                     HIKLQGQSPVRGNNIPEFGPRFFDVSDMYTRDLRDLAKRIGDKEDIKLKEGVYFYATG
                     PQFETPAEIRAMRILGGDAVGMSTVTEAITAAHCGIKVLGISLISNMAAGITGNPVTT
                     QEVNEIGELSEDKVERLIREIIKEI"
     gene            569..1627
                     /locus_tag="VFD79_03445"
     CDS             569..1627
                     /locus_tag="VFD79_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HZH93133.1"
                     /translation="MERQDRDLAFMLRYENVAWYEDGKVKILDRRVYPTSVRYEVCNS
                     HQEVAQAVTDMVTQSAGPYTAAGMGMALAAYEAREMKEVDQLEYLRKAAETIATARPT
                     TTKRMRIIVEGCLEVAETALEEGKRVDEAIFLHTVDHTENRYKRIGKVAVNLVDLFPD
                     KGTIMTQCFGETIVGLMLREAKERNKDIKVICPETRPYFQGARLTASVAQDQGFHTTV
                     ITDNMPAYVMEKKKVDLFTSAADAICLDGHIVNKIGTYQIAIVAKHLGIPYFVTGIPD
                     KGHDTIDTVTIEERDPEFVLQAMGVRTAMEGVNGFYPSFDATPPHLVSGVVTDRGIFS
                     AYDLHRYFKEGEKAELVL"
     gene            1711..2895
                     /locus_tag="VFD79_03450"
     CDS             1711..2895
                     /locus_tag="VFD79_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZH93134.1"
                     /translation="MNLKYAERMKNMKASEIRELLKVAAQPGIISFGGGFPAPELFPV
                     EEMIAVSKQVIEEKGSQALQYGPTEGYQPLRAAIAKRMEKMQAFVEDKDILVTSGSQQ
                     GLDFSAKLFINPGDTIILEKPSYLGAINAFKAYEPNFIDIELEDDGMNMEELEEALKT
                     GSNVKFIYVIPDFQNPSGKTWSVEKRKKLVELGNKYDVAIIEDNPYGELRFEGELLPS
                     IKHFDTDGRVIYLGTFSKILCPGFRLGWIAASPEILNKYVLMKQGADLQSSTISQMEV
                     AMFLEKYDIEEHIEKIKEVYLRRRNLMINVMKEEFPEGVTYTIPEGGLFTWVTLPEHI
                     NTRDLAVKALEKKVAFVPGGSFYANGGHENSMRLNYSAMEDEKIVEGMKLLAGVIKES
                     MK"
     gene            2952..3383
                     /locus_tag="VFD79_03455"
     CDS             2952..3383
                     /locus_tag="VFD79_03455"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HZH93135.1"
                     /translation="MVRRKTLDKKYIYIRDGQKCFYCGKALKPGQMTLDHYQPRSKGG
                     TDDYFNLVSCCRLCNKYKKNKIPVDKEKIHLELFHRAWKDKRIIWAVKGTGRKESEEI
                     IKRITSIQCVEEESIARGDRITLYIHNNVIIRMDGDRMGIL"
     gene            complement(3386..3928)
                     /locus_tag="VFD79_03460"
     CDS             complement(3386..3928)
                     /locus_tag="VFD79_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93136.1"
                     /translation="MSEDRSKIANQVEEIKNKATAALAKQINFYGLNLSESRLFSLMF
                     LEDSPMTLDEMSGQLGMSKTSMSTGIHSLQDAKMVEQTWRKGVRKDLYKAEENLYSSF
                     SNTYLEKWFSVIGNNKKIFNSISRDIATLLDKVEEDDLRESLVSYSLKIGEVLQFYTW
                     LESMFRDMRDSIDKMDFERD"
     gene            4304..5497
                     /locus_tag="VFD79_03465"
     CDS             4304..5497
                     /locus_tag="VFD79_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HZH93137.1"
                     /translation="MKKIVVEDLTKVFGKNPKLGIKLLKEGKSKSEILEKTGMTVGVN
                     RVSFEVNAGEVFVIMGLSGSGKSTLIRLVNRLIEPSHGSIMIDGEDLSKMDKKKLIET
                     RRKKLSMVFQNFGLFPHRTIIENTGYGLEVQGYEKSEIQEKSKRALEMVGLKGYEDQY
                     PNQLSGGMQQRVGLARALANDPDILLMDEAFSALDPLIRKDMQDELLELQQSMKKTIL
                     FITHDLDEALKIGDRIALMKDGLIVQIGTPEEIMTNPSNDYVEKFVEDVDRSKVFTAA
                     HVMIKPETIQIEKHGPRVALQRMKKAGISSVYVTKNNQELYGIVTAEAAAQAIDNGVK
                     DLEDIVEKEVPTVPPDISLNDLFKVIYDTPIPVAVTEGKVLKGIIVRGAVLAALSGNE
                     VNLDE"
     gene            5490..6329
                     /locus_tag="VFD79_03470"
     CDS             5490..6329
                     /locus_tag="VFD79_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline/glycine betaine ABC transporter
                     permease"
                     /protein_id="HZH93138.1"
                     /translation="MNNIPQLPLVRWVDTMVRYLRSNARWFFDPISDFLDTFVDGVSA
                     VLMFLPPLAFIALMVIVTIYLTKKVWGLPVFVLLGLTLIWNLNYWEGTMLTLALILSA
                     SLIAIVFGIPLGIWMAKSETVESIVKPILDFMQTMPAFVYLIPAVSFFGIGMVPGIIA
                     SVIFAMPPVVRLTNLGIREVSRELIEASDAFGSTGTQKLFKVQLPLAKSTIMQGINQT
                     IMLALSMVVIASMIGAEGLGTEVYRAIGRNQAGQGFASGIAIVILAIILDRLIQVMNK
                     KKY"
     gene            6394..7287
                     /locus_tag="VFD79_03475"
     CDS             6394..7287
                     /locus_tag="VFD79_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HZH93139.1"
                     /translation="MFNRNFKKVALVAIAVFALALAGCASDGGNGGEAGSLGDQLNYT
                     ITGIDAGAGVVRSAEQAVEDYGLPYTVQTSSGAAMTQALGDAIANEEPIIVTGWNPHW
                     KFAAYDLKYLDDPKGSFGEAEDIHSIARMGLSEDMPEAYQILDNFYWEGSDMEDVMIE
                     VNDGAEPADVARQWVDDNQDKVAEWTEGVDPVDGVEINLAYVAWDSEIASTNVIGTVL
                     SDMGYDVTLTQVENGPMWEAVASGQTDAIVAAWLPGTHGDHYDNYEGEFDDLGANLEG
                     ARIGLVVPAYLDINSIEDLVE"
     gene            complement(7334..8392)
                     /locus_tag="VFD79_03480"
     CDS             complement(7334..8392)
                     /locus_tag="VFD79_03480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZH93140.1"
                     /translation="MEKRIYVYLAAIMTITMLVTQVVLSFLIKDYYSNLADPGIVTIW
                     EIFLAMIPAMIGILVFLLVILFVLSSKLTQLILAPIKRATDNIESILSGKKVQEASIY
                     PEVRPFIQTIDHQKRQIDMALSELREAEKIRRDFTANVSHELKTPLTSINGFAEMIAS
                     GTTDKDDTIKFAGIIRKEGTRLLNLIDDIISLSRLDGAPKAQEFEKLQLDRISEDIIN
                     QLRIRARDKEVALEFVGEPVWIDGGGRMIEDLIYNLVDNGIKYTENGGRVTLSVFPES
                     DGAIIQVSDTGIGIPDEDQSRIFERFYQVDKSRSKKVGGSGLGLSIVKHIVQSHGGSI
                     DLKSSLGKGTTIKVRLPK"
     gene            complement(8393..9064)
                     /locus_tag="VFD79_03485"
     CDS             complement(8393..9064)
                     /locus_tag="VFD79_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005396942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZH93141.1"
                     /translation="MSLIYCVEDDESIRELILYALKNAGFEAEGFESGKELDLGKMPD
                     ILLLDIMLPGEDGYEILSRIRRNPKTSDLPVIMLTSKTSEFDKVRALDMGADDYMDKP
                     FGVMELISRIRAVLRRSGGKKTPDKLCINGICLDWEKHRVTAEDKEVVLTPKEFDLLQ
                     YLMENQGIVLSRDKIMNQVWGFDFEGETRTVDVHIRTLRMKLGDSGESVQTVRGIGYR
                     FGGDF"
     gene            complement(9070..9720)
                     /gene="phoU"
                     /locus_tag="VFD79_03490"
     CDS             complement(9070..9720)
                     /gene="phoU"
                     /locus_tag="VFD79_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HZH93142.1"
                     /translation="MRSRFDQELDTLNEELVSMGNLVESSIEAAVSALKTQNVDLARR
                     VVEGDREVNNKEREIEDLAMKLILRQQPVAGDLRLISTALRMITDMERIGDQAADISE
                     ITIMLADQTYLKELVHIPQMGDATIKMVRESIDAFVRKDVELAKQVILYDDVVDDLFD
                     VIKEELIGYIRENKGSSEQAIDFMMIAKYFERIGDHAQNIAEWVYYAITGDHYNGS"
     gene            9948..10232
                     /gene="rpsF"
                     /locus_tag="VFD79_03495"
     CDS             9948..10232
                     /gene="rpsF"
                     /locus_tag="VFD79_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019108155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HZH93143.1"
                     /translation="MRKYEAMVIFYPNLEEEKRNALLERFKKIIEAGGTVTTVDEWGM
                     RKLAYLIDDLAEGYYVLINFESGADVVNEFDRIAKISDGIMRHMITRDEE"
     gene            10253..10684
                     /locus_tag="VFD79_03500"
     CDS             10253..10684
                     /locus_tag="VFD79_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZH93144.1"
                     /translation="MNNVVLIGRLTRDPELRYIPNSGTPVSTFTLAVDKNLSKEKKME
                     MESKNQPTADFIRIVVWGKQAENCANFLNKGKLVGINGRIQTGSYDDKDGKRVYTTDV
                     VANSVEFLEWGDNNQKSSGSTGSDFSDIDGFHPTDNDDIPF"
     gene            10702..10926
                     /gene="rpsR"
                     /locus_tag="VFD79_03505"
     CDS             10702..10926
                     /gene="rpsR"
                     /locus_tag="VFD79_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009221815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HZH93145.1"
                     /translation="MQRRFKPRKKVCSFCADKSTHIDYKDVSKLKKYITERGKILPRR
                     ISGNCSKHQRQLTIAVKRARQVALLPYSAD"
     gene            11068..12033
                     /locus_tag="VFD79_03510"
     CDS             11068..12033
                     /locus_tag="VFD79_03510"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HZH93146.1"
                     /translation="MRFLKDDNKMVQPVIEGAGATALVVLVGLLSIYLTPLVLILYPV
                     GAVVYGIKNGVNPAAGVITIASLIIGLGTGDFISGIILLILFGPLTVALAYGMKKRRR
                     PLQVLVTGSLVFFTSTLMVFYLADSLTGLSIINQLENAFKETLFIQIDMLKGMGLSNY
                     EIDSARRMLEDGYRYIILILPSILMFLALLVSHVNYIITVWLLRKLNIGILQMPWLHR
                     FTVPNNFALGVLVMFAGVFIGRSLDGTYYETIFLNLLVLIGVVFILQGLAVVSFFLIK
                     WKMNAFTRGLVVFLAIAFSPVLTAVSLIGGLDVVLDFRKLRRKPQ"
     gene            12044..14035
                     /locus_tag="VFD79_03515"
     CDS             12044..14035
                     /locus_tag="VFD79_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HZH93147.1"
                     /translation="MKYKIFTWSDTKLYLLVIGLSVLIIALFQPIVAFVMGLVLAYLV
                     YYNHRKIEEKNKELAKYIEGLSHDFDSAAKHAIFNMPFPMVFIDEDGKITWYNTPFLT
                     LLGEEEIVNKSISELVPDFNLSDLRKKKENEPMSISYLDRYFDVYPNFLEDRKTSGKG
                     SITVLYWVDDTDHVVLEKEHNRDKTVVALVDVDNYDDVKNSTPDIHRPIVLAEIDQLI
                     NSYFSQYEGIVRKYENDKYMVVLNQGGLEAIKGKRFDLLDQIRDMDMGNTIPITLSIG
                     VCQAGKSLIESYGNASAAIDIALGRGGDQAVINVGNAFDYYGGKSKAMEKRNKVKARV
                     IGDALRQLIDQNERVFVMGHKNPDMDSIGSSVGIMRAIDNRNKEAYLVLNGENPSIRN
                     LMDRMRKDSPDMVKRVVTGDEARDLVDERSLLIVVDNHKPSLTEAPELLELTQKVVVI
                     DHHRRGAEFINNPVLTYLEPYASSTSELVTEVLTYMGEETNLTKFEAEALLAGITVDT
                     KNFSFQTGVRTFEAASVLKRAGADTTVVKQLFRDDFSTFVNKAEVIKSSKIVFDRIAI
                     GRLQNKMEDSILIAAQAANELLNINDVEASFVLSQLEDKIHISGRSLGNISVQLILEK
                     LGGGGHLTSAGAQVEKLSMDEAEKLLTDIIDKYLKEGEK"
     gene            14035..14481
                     /gene="rplI"
                     /locus_tag="VFD79_03520"
     CDS             14035..14481
                     /gene="rplI"
                     /locus_tag="VFD79_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HZH93148.1"
                     /translation="MKVILLKDVKGMGKAGDLVNAKDGYSRNYLIPRNIAIEATADNL
                     RKWEESKAKEAANKEQEMKEALELKEKIEKLTVNIKAKGGTEGRLFGSVTSQDIAAGL
                     KKQHKIDIDKRKIELKDNIKTTGVTEVEVKVYPDVSATLKVDVAAE"
     gene            14494..15840
                     /gene="dnaB"
                     /locus_tag="VFD79_03525"
     CDS             14494..15840
                     /gene="dnaB"
                     /locus_tag="VFD79_03525"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009431263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HZH93149.1"
                     /translation="METGIGRIPPHSMEAEQSVIGAMLLDKEAINTAIEHLRGDDFYK
                     EANKEIYEAMLVLNNRNEPVDLVTLSEELKRRGSLEGVGGVTYLANLSGSVATTANTK
                     YYCDIVQEKSILRRLIGSSNEIMGLAYDNSDSVNSIIERAEKNIFDITQGAHKQSFHP
                     ISEVLLSSFAQIEERAANKGELTGLTTGFSDLDHKLSGLQKSDLVLLAARPSMGKTAL
                     GINIATNASLKGNGKVAVFSLEMSKEQLVQRIISATAHVDLQKIISGNLSDDEWIKVV
                     NAMAPLSKMDIYIDDTAGISLMEMKAKCRRLKVEKGLDLIVIDYLQLMQADGRVENRQ
                     QEISAISRGLKAIAKEMECPVLALSQLSRAPELRSDHRPILSDLRESGAIEQDADVVM
                     FLYRDDYYDQESEKKNVGEVIIAKHRNGPTGQVDLVFKHEYTKFLNMERYIEPGNG"
     gene            15989..>16545
                     /locus_tag="VFD79_03530"
     CDS             15989..>16545
                     /locus_tag="VFD79_03530"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZH93150.1"
                     /translation="MIIKGKRVTLSPLTMEDAKSMKGWGVHETVLLGDYNFPFENDIE
                     VKRWFRYKTFTIWNKYFGVHNEEGNLVGYLGIKNKDFIRRSAYLGIVFDPDYMDRGYG
                     TESLEAFLPKYFNGMRMKTLYLEVAEFNKRALRVYEKLGFIPIQYYTDLFPVEGISKD
                     HPDIMASKSCFVFKDEKIYNYIYKM"
BASE COUNT         5073 a         3310 c         3980 g         4182 t
ORIGIN      
        1 tatgactttg aaagacttgc tataccaata agggtattca ggcttcttgg agttgaaacc
       61 cttgttctca caaatgcagc tggagcaatc aatactgata tggtgccagg ggacattatg
      121 ctaataaatg accatataaa gctgcagggt cagagtcctg tgagagggaa caacatccct
      181 gagtttgggc caagattctt tgatgtctct gacatgtaca ccagagacct tagggacctt
      241 gcaaagagga taggagataa ggaggacatc aagctcaagg agggggtcta tttctacgca
      301 acaggacccc agttcgagac cccggctgaa ataagggcca tgaggattct cggtggagat
      361 gccgtcggca tgtccactgt aacagaggca ataacagctg cccattgtgg gatcaaggta
      421 cttggaatat ccctgatttc aaatatggca gcgggaataa ccggaaaccc agttaccacc
      481 caggaggtaa atgaaatcgg tgaattgtcc gaagacaagg ttgaaaggct tataagggaa
      541 ataatcaagg aaatctagga ggtgcacaat ggagagacag gacagggacc tggcctttat
      601 gctacgctat gaaaatgtgg cctggtatga ggacgggaag gtcaagattc ttgataggag
      661 ggtttatcct acctcggtca ggtatgaggt ttgcaatagt caccaggagg tggcccaggc
      721 agtgaccgat atggtgacac aaagtgccgg accctacacg gcagccggaa tgggaatggc
      781 cctggcagcc tatgaggcca gggagatgaa ggaagtggac cagctggagt acttaagaaa
      841 ggctgcagag accatcgcca cagcaagacc caccacaaca aagaggatga ggatcattgt
      901 ggagggctgt cttgaggttg cagagactgc attagaggaa ggtaaaaggg ttgatgaggc
      961 aatcttccta cacactgtag accatacgga aaacaggtac aaaagaattg gcaaggttgc
     1021 cgtaaacctt gtggacctgt ttccagacaa gggaacaatc atgactcagt gctttggaga
     1081 gacaattgtt ggtctgatgc taagggaggc aaaggaaagg aacaaggaca taaaggttat
     1141 atgtccagag accaggccat acttccaggg agcaaggcta actgccagcg ttgcccagga
     1201 ccagggcttc cacaccaccg ttataactga caacatgcca gcctatgtca tggaaaagaa
     1261 gaaggttgac ctattcacat cagctgcgga tgccatatgc ctggatggcc acatagtcaa
     1321 caagattggg acctaccaga tcgcaattgt tgccaagcat cttggaattc catactttgt
     1381 aacaggaata cccgacaagg gtcatgatac cattgacact gtaacaatcg aggagaggga
     1441 tcccgagttt gttcttcagg caatgggggt gagaactgca atggagggag tcaatggatt
     1501 ctatccatcc tttgatgcaa caccacccca tctagtcagt ggagtggtca ctgacagagg
     1561 catattctca gcgtacgacc tccacaggta tttcaaggag ggagagaaag cagagctggt
     1621 cctctaatgg gtctggacaa ttgaaacaag aaagtgtaaa ctataggtaa tatgggaaaa
     1681 ggttttccga agaaaaatgg aggtgctgta atgaatttga aatacgcaga gagaatgaaa
     1741 aacatgaagg cttcagaaat aagagagctt ttgaaggttg ctgcccagcc tggaatcata
     1801 tcatttggtg gaggttttcc tgctccagag ctgtttccag tagaagagat gatagccgta
     1861 tctaagcagg tcattgaaga aaagggatca caagcccttc aatacggacc tacagaaggt
     1921 tatcagcccc taagagctgc aatagcaaag agaatggaga agatgcaggc atttgtagaa
     1981 gacaaggata tccttgtaac aagtggttcg cagcaaggat tggatttctc agcaaagctg
     2041 ttcattaacc caggagacac aataatactt gaaaagccaa gctatcttgg agctatcaat
     2101 gcattcaagg cctacgagcc aaactttatc gacattgagc ttgaggatga cggaatgaat
     2161 atggaagagc ttgaagaggc ccttaagaca ggctcaaatg ttaagttcat ctacgtgata
     2221 ccggattttc agaacccatc aggcaaaacc tggtctgtgg aaaagagaaa gaagctagtt
     2281 gagcttggaa acaagtacga tgttgcaata attgaggata acccatatgg agagcttagg
     2341 tttgaagggg aattgcttcc atcaatcaag cattttgata cagatggaag ggtaatctac
     2401 ctgggaacat tctcaaagat tctttgtcca ggattcagac ttggatggat tgccgcatca
     2461 ccggagattc taaacaaata tgtacttatg aagcagggag cagacctaca gtcaagtaca
     2521 atatcacaaa tggaagttgc catgttcctt gaaaagtatg atatagagga gcacatcgaa
     2581 aagatcaagg aagtctacct gagaagaaga aaccttatga taaacgttat gaaggaagaa
     2641 ttccctgagg gagttaccta tacaatacct gagggtggac tcttcacatg ggttacccta
     2701 cctgagcaca tcaatacaag agaccttgca gtaaaggctc ttgagaagaa ggttgcattt
     2761 gtgccaggag gatcattcta tgcaaatggt ggtcatgaaa acagtatgag actaaactat
     2821 tctgcaatgg aagacgaaaa gattgtagaa ggaatgaagc ttcttgcagg tgtaataaag
     2881 gagtcaatga agtagaagaa aaccccggaa atttatttcc ggggaatttt tttgtcgggg
     2941 gggagagctt tatggtaagg agaaagactc ttgataagaa atacatatac attcgggatg
     3001 ggcagaagtg cttttattgt ggaaaagctc tcaagcctgg acaaatgacc ctggaccact
     3061 atcaaccccg ctcaaaaggg ggaactgacg actatttcaa cctggtatcc tgctgcaggc
     3121 tgtgcaataa atacaaaaaa aacaaaatcc ctgtggataa agaaaagatc catttggaac
     3181 ttttccacag ggcatggaag gacaaaagaa tcatatgggc ggtgaagggt acagggagaa
     3241 aggaatccga agaaataata aagaggatca ccagcatcca atgtgtggag gaagaatcca
     3301 ttgctcgtgg agacaggata accctttaca tacataacaa tgtaataatc aggatggatg
     3361 gagacaggat gggtatccta tagacctagt ctctctcaaa atccattttg tcgatactgt
     3421 ccctcatgtc cctgaacatt gattccaacc aggtgtagaa ctgaaggacc tcaccaatct
     3481 tcaatgaata actcaccaga gattccctta agtcatcttc ttcaacctta tccagcaggg
     3541 ttgcaatatc cctggagatg gagttgaaga tttttttatt gttgccaatt acggaaaacc
     3601 atttttccag ataggtattt gagaagctag agtaaagatt ttcctcagcc ttgtaaaggt
     3661 cctttctgac accctttctc caggtctgtt caaccatctt ggcatcctgt agactgtgaa
     3721 ttcccgtgct catggaggtc ttgctcatgc ccagctgtcc gctcatttca tccagggtca
     3781 tgggggagtc ctccagaaac atcagggaga acaaccttga ttccgaaagg ttcaatccat
     3841 agaaatttat ctgctttgca agggctgcag tagccttgtt ctttatttcc tcaacttgat
     3901 ttgcaatctt tgatctatcc tctgacattt tatcctccta attgtttctc ggcgtttaac
     3961 aagctaaagt acactattta cttctaatat agattattat tcaaacaaaa tcaatatgga
     4021 taatataggg aaaatagtgt ataatcatta ttgtacagaa aaaactgaac aaacgcaaca
     4081 tacaatacaa acggaataat cccttgacaa tttatgaaaa acctgtataa tagataaatg
     4141 gagcctcaaa gaaattctgt tgaggttttt tctgaccaaa atcaggaata tttcgattgg
     4201 atctcttgtt ttagggaagg ttgctgtata agtatggacc agtcaacggg gattcaactg
     4261 atataaaata taaaattaag atgtatgaga acggggtgaa tggatgaaaa aaattgtagt
     4321 tgaagatttg acaaaggtct tcgggaagaa tcccaagctt gggatcaagc tactgaagga
     4381 aggcaagtca aagtctgaga ttcttgagaa gacaggcatg actgttggag tcaacagggt
     4441 ttcctttgag gtgaatgccg gtgaggtgtt tgtaataatg ggcctgtcag gaagtggaaa
     4501 atcaaccttg atccgtttgg tgaacagact tattgagcct tcacatggaa gtataatgat
     4561 agatggtgaa gacctgtcaa agatggataa gaaaaaattg attgaaacca gaaggaaaaa
     4621 actgagcatg gtattccaga atttcggttt attcccacac agaacaatta ttgagaacac
     4681 tggctatgga ctggaagtcc agggctatga aaaatctgaa atccaggaaa aatccaaaag
     4741 ggcactggag atggtaggcc tcaagggcta tgaagaccag tatcccaacc agctttccgg
     4801 aggaatgcag cagagggttg gattggcaag agcccttgca aacgacccgg atattcttct
     4861 tatggatgag gcattttcag cacttgaccc gctgatcaga aaggatatgc aggatgagct
     4921 tcttgagttg cagcagtcaa tgaagaagac catactattt attacccatg accttgatga
     4981 ggcacttaag atcggggaca ggatagccct tatgaaggat gggttaattg ttcagatagg
     5041 tactcccgag gagatcatga caaatccttc aaatgactat gtagagaaat ttgttgagga
     5101 tgttgacagg tcaaaggttt tcactgcggc acatgtaatg ataaagcctg agaccataca
     5161 gattgaaaaa cacggtccaa gggttgccct gcagagaatg aagaaggctg gaatatccag
     5221 cgtatatgta acaaagaaca atcaggagct ttatggaata gttacagctg aggcagctgc
     5281 acaggcaatc gacaatggcg taaaggacct tgaagatatt gtagaaaagg aagttccaac
     5341 agtaccacct gatatatcct tgaacgactt gtttaaggta atatacgaca ctcccatacc
     5401 cgttgcggta accgaaggta aagtactgaa gggaataatc gtccgaggag ccgttctggc
     5461 tgccctgtcg ggaaacgagg tgaatctgga tgaataatat accacaatta cctttggtga
     5521 gatgggtaga tacaatggta agatacctga gaagcaatgc aagatggttc tttgatccca
     5581 tcagtgattt tttggatacc tttgtagacg gtgtaagtgc agtcttgatg ttcctgccac
     5641 cactggcctt tatagccttg atggtgatag ttacaattta ccttacaaag aaggtgtggg
     5701 gactgccggt atttgttctg ctgggtctaa cccttatttg gaacctcaac tattgggagg
     5761 gaacgatgct taccctggca ctgatacttt cggccagcct tatagccatt gtctttggta
     5821 ttccactggg aatatggatg gctaaaagtg aaacggtaga gagtattgta aagccgattc
     5881 ttgacttcat gcagacaatg cccgcatttg tttacctaat acccgcagtt tcattctttg
     5941 gaatcggaat ggttcctgga ataattgctt ctgttatttt tgcaatgcct cctgtcgtaa
     6001 gacttacaaa ccttgggatt agagaggttt caagggagct tattgaagca tccgatgcat
     6061 ttggatcgac aggaacacaa aaactgttca aggttcagct tccacttgcc aagagtacaa
     6121 taatgcaggg aatcaaccag acaatcatgc ttgccctatc aatggtggta atagcatcca
     6181 tgatcggtgc agagggtctt ggaacagaag tgtatagggc aattggtaga aaccaggcag
     6241 gtcaaggatt tgcatcaggt attgcaattg taatacttgc catcattctt gacaggctga
     6301 tacaggtaat gaacaagaag aagtactgat ttgggacaac acttatttca atacggaaat
     6361 aagtaatgat atatcaaagg aggaatttag tttatgttta acaggaattt taagaaggtg
     6421 gcattggttg ccatagcagt ttttgccctt gccttggcag gatgtgcaag cgacggtgga
     6481 aacggtggag aagcgggatc tcttggagac cagcttaact acacaattac aggaatcgat
     6541 gcaggagctg gagttgtgcg ttcagcagag caggcagttg aggactatgg actaccatac
     6601 acagttcaga ctagttcagg tgctgcaatg acacaggcac tgggtgatgc aattgccaac
     6661 gaagagccaa tcattgttac tggctggaat ccacactgga aatttgcagc ctatgacctt
     6721 aagtatcttg atgatccaaa gggatcattc ggagaagcag aagacatcca ctcaattgca
     6781 agaatgggat taagtgaaga tatgccagag gcataccaga tacttgacaa cttctattgg
     6841 gaaggatcag acatggaaga tgttatgatc gaagtaaacg atggtgccga gccagctgat
     6901 gttgcaagac agtgggttga cgacaatcag gacaaggtag cagagtggac tgaaggagta
     6961 gacccggttg atggtgttga gatcaacctt gcatatgttg catgggattc tgaaattgca
     7021 tcaacaaatg taataggaac tgtactttca gacatgggtt atgatgtaac ccttacccag
     7081 gttgaaaacg gaccaatgtg ggaagctgta gcatcaggac agactgatgc aatagtagct
     7141 gcatggttgc ctggaacaca cggagaccac tatgacaatt atgagggaga gtttgacgat
     7201 cttggtgcga accttgaagg tgcgagaata ggtctagtag ttcctgcata tttggatatc
     7261 aattcaatag aagatctagt ggaataattt ggacattaaa atggtaaccc tttgggttac
     7321 catttttttc aacctatttt ggaagcctta cctttattgt tgttcccttg ccaaggctac
     7381 tcttaaggtc aattgaacca ccgtggcttt ggacaatgtg cttgacaata gaaagcccaa
     7441 gaccagaccc accgactttt tttgaccggc tcttgtcaac ctgatagaat cgttcaaaaa
     7501 tccgagactg gtcttcatcg ggaatcccta tccccgtatc agatacctgg attatggctc
     7561 catcagattc tggaaacact gacaatgtaa cccttccgcc attctccgta tatttgattc
     7621 cattgtccac aaggttatat ataagatcct caatcatcct cccgccgcca tctatccaaa
     7681 ccggctctcc cacaaattca agggcaactt ccttgtccct tgcccttatc ctaagctggt
     7741 tgatgatatc ctcggaaatc ctgtccagtt gtagcttctc aaactcctgg gcctttggag
     7801 ctccatcaag ccgtgaaaga ctgatgatat catcaatcag gttcaagagc ctggtgcctt
     7861 ccttgcgtat tattccggca aatttgattg tatcgtcctt gtccgtcgtt cctgaagcta
     7921 tcatttccgc aaatccattt atgctggtca ggggcgtctt taactcatgg gatacatttg
     7981 ctgtgaaatc gcgacgaatc ttctccgcct ccctaagctc acttaaggcc atgtcaatct
     8041 gcctcttctg atggtcaatt gtctgaataa atggcctcac ctccggatat attgatgctt
     8101 cctggacctt tttgccggaa aggatgcttt caatattgtc tgttgccctc tttatgggtg
     8161 caagtatcag ttgggtcaac ttgctggata ggacgaacag gattaccaga agaaatacca
     8221 gtatacctat cattgcaggt atcattgcca ggaagatttc ccaaattgta acaatgcctg
     8281 gatccgcaag gtttgagtaa tagtccttga tcaggaatga aagcacgact tgggttacaa
     8341 gcatggtaat ggtcattatg gctgcaagat aaacataaat cctcttttcc atctaaaaat
     8401 cacctccgaa cctgtagccg ataccacgaa cggtctggac agattctcca ctgtcaccca
     8461 acttcatgcg aagggtcctg atatggacat ccacagtacg ggtttctccc tcaaaatcaa
     8521 aaccccatac ctggttcatt atcttatccc ttgaaaggac tatcccctgg ttttccataa
     8581 gatattggag gaggtcaaac tccttggggg tcaggacaac ttccttatcc tcagcagtta
     8641 ccctgtgttt ttcccagtca agacatattc cattgatgca aagcttatca ggggtctttt
     8701 tccctcctga ccttcggaga acagccctta ttcttgaaat cagctccata accccaaagg
     8761 gcttatccat gtagtcgtca gcacccatgt caagagccct caccttgtcg aattcgctgg
     8821 ttttggaggt aagcattatt acaggcaagt ccgaggtctt ggggtttctt cgaatccgtg
     8881 acagaatttc atagccatcc tctccaggca gcatgatatc aaggagtaga atatccggca
     8941 ttttacccaa gtccagctcc ttgccgcttt caaagccctc tgcctcaaag cccgcattct
     9001 taagggcata caaaataagc tcccttatgc tctcgtcgtc ctcaacgcaa taaatcagac
     9061 tcattcctat caactcccgt tgtaatggtc tcctgttatg gcatagtaaa cccactctgc
     9121 aatattctga gcatggtcac cgattctttc aaaatatttg gcaatcatca taaagtctat
     9181 ggcctgctca ctggagccct tgttttccct aatataccca atgagctcct ccttaataac
     9241 atcaaatagg tcatcaacca catcgtcgta gaggattacc tgctttgcca gttccacatc
     9301 cttccttaca aatgcatcga tgctctcccg caccatctta attgtagcgt ctcccatctg
     9361 ggggatgtgg accaattcct taaggtaggt ctggtctgcc agcatgattg tgatttcaga
     9421 aatgtccgca gcctggtccc ctatcctttc catgtccgtg atcattctaa gggcggtgga
     9481 aatcaatctc aggtcccccg caacaggctg ctgacgaagt atcagcttca ttgcaaggtc
     9541 ctcaatttcc ctttccttgt tgtttacctc cctgtctccc tccacaaccc gtcttgccag
     9601 gtcaacattc tgggttttaa gtgccgatac agctgcttca atggagctct ccacaaggtt
     9661 tcccatggag accagctctt cgttgagagt atccaattct tgatcaaatc tgcttctcat
     9721 ataaaccatc ctttattagt attttctatt gtaattataa tacccaactg taaagatacc
     9781 tcttgcaata tgtaaagttt atgttaaatt taattataga gggtggaaat ccaacccaaa
     9841 aacatgacaa aacatttgaa aaagagccca agttgtgtta tactacttta gtgttccttg
     9901 ccctattaat gaaaataggg ccgatagtcc atagggaggt gaaataaatg agaaaatatg
     9961 aagcgatggt aatcttttac cctaatcttg aggaagaaaa aagaaacgct ttgcttgaga
    10021 gattcaagaa gataatcgag gcaggcggta cagtaacaac agtagatgaa tggggaatga
    10081 gaaagcttgc atatctgatc gacgatttgg ctgaaggata ctacgtactg attaactttg
    10141 aatcaggagc ggatgtagtc aatgagttcg atagaattgc caagatttca gacggcatta
    10201 tgagacacat gatcactaga gacgaagaat aatctaaggg acggtgagta ttttgaataa
    10261 tgttgttcta atcggaagac tgacaaggga ccctgaactg agatatatac caaattcagg
    10321 aactccagtg tcaacattca cgttggctgt agacaagaat ctttcgaagg aaaagaaaat
    10381 ggaaatggaa tcaaaaaatc agcctacagc tgatttcatt agaattgtcg tatggggaaa
    10441 gcaggcagag aactgcgcca acttccttaa caaggggaaa ttggtcggga taaacggcag
    10501 gattcagact ggaagctatg atgataagga tggaaagcga gtttacacaa ctgatgttgt
    10561 ggcaaacagc gttgagttcc ttgaatgggg agacaacaac cagaaatcat caggttcaac
    10621 aggctcggac ttctccgata ttgacggatt tcaccctacc gacaatgacg atattccatt
    10681 ctaatcaagg aggggaaaag gatgcaaaga aggtttaagc ctagaaagaa agtgtgtagc
    10741 ttttgtgcag acaaatcaac acatatcgac tataaagatg taagcaagct taagaagtat
    10801 ataactgaaa gaggaaagat tcttccaaga agaatttcag gaaattgctc aaagcatcaa
    10861 agacaactta caattgcagt caagagagca agacaagttg ccttgttgcc atatagcgct
    10921 gactaagaga gagggaaacc tctctttttt ctttacggtt atacaaagac cacaagcggg
    10981 cttttaatta tattggaatt aatatataat caatgatagg catactttgg acaatgacct
    11041 gaaatgacac tattgagcag acttgaggtg agatttttga aagacgacaa taaaatggtt
    11101 caaccagtga tagagggagc aggagcaacg gcacttgtgg tactggttgg cctcttatca
    11161 atttacctga cccctctggt actcattctc tacccggtag gtgcagtggt atacggaatc
    11221 aagaacggtg tcaacccagc ggctggcgta ataacaatag cctccctgat aattggattg
    11281 ggcactgggg atttcatcag cggaattatc ctactgatac tctttggacc acttacagta
    11341 gcccttgcat atgggatgaa aaaacgaagg aggcccttac aggtacttgt gaccggatcc
    11401 ctggtttttt tcaccagtac actgatggtg ttctaccttg ccgattccct gactggcctg
    11461 agcataataa accagctgga gaacgccttt aaggagaccc tattcatcca aattgacatg
    11521 ctcaagggta tggggcttag caactacgag atagacagcg ccagaaggat gctggaggat
    11581 ggatacaggt atatcatatt gatactaccc tccatactta tgttcctggc acttttggtt
    11641 agccatgtaa attatatcat tacagtatgg ctcctaagga agctgaacat cggaatattg
    11701 cagatgccat ggcttcatag gtttacagta cccaacaatt tcgcccttgg agtattggtg
    11761 atgtttgcgg gagttttcat tggcagaagt ttggatggaa catattatga aacaatattt
    11821 ctgaacctgc ttgtcctgat aggtgtagtt ttcatactac agggacttgc agtcgtaagc
    11881 ttcttcttga tcaaatggaa gatgaacgct tttacaagag gattggttgt atttttggcc
    11941 attgcatttt ccccggtatt gaccgccgtc tcccttattg gcggattgga tgtagtgctc
    12001 gatttcagga agctaagaag aaaaccccag taggaggaag aagatgaaat ataaaatatt
    12061 tacctggtct gataccaagc tgtatctgct ggtcataggc ctaagcgtac ttataatagc
    12121 actgtttcag ccaattgtag catttgtaat gggcttggtc ctggcatatc ttgtatacta
    12181 caatcacaga aagattgagg aaaagaacaa ggagcttgca aaatacattg aaggcctttc
    12241 ccatgatttt gactcagctg ccaagcatgc catcttcaac atgccctttc ccatggtctt
    12301 tatagatgag gatggaaaga taacatggta caatacgcca tttcttacct tgctgggcga
    12361 ggaggaaata gtgaacaagt caatcagcga acttgtacct gacttcaatc tttcggacct
    12421 taggaagaag aaggaaaacg agccaatgtc gatcagctac ctggacaggt atttcgatgt
    12481 ttacccaaac ttcctagagg acaggaaaac ctctggaaag ggcagcatca ctgtgctcta
    12541 ctgggtagac gatactgacc atgtggtcct ggagaaggag cacaataggg ataagacggt
    12601 tgttgccctt gttgatgtgg acaactatga tgatgtcaag aactcaacac cggacattca
    12661 cagacccatt gtacttgctg aaatagacca gcttataaac agctacttca gtcagtatga
    12721 aggaatagta aggaaatatg aaaatgacaa gtatatggtt gtgcttaacc agggtggtct
    12781 tgaagccatt aagggcaaga gatttgacct cttggaccag atcagggaca tggacatggg
    12841 gaatactatc cccatcacac tgagcattgg agtctgtcag gctgggaaaa gcctgattga
    12901 gtcctacgga aatgccagtg ctgccattga catcgccctt ggtaggggag gagaccaggc
    12961 tgtcataaat gtaggaaacg cattcgacta ctacggtggc aagtccaagg caatggaaaa
    13021 gaggaacaag gtaaaggcca gggtaattgg agatgcccta agacagctta ttgaccagaa
    13081 tgaaagagtg tttgtaatgg gacacaagaa tcctgacatg gactccatag gctccagtgt
    13141 gggaatcatg agggcaatag acaacaggaa caaggaggca tatctcgtac tgaacggtga
    13201 gaatccatcc attagaaacc tgatggacag aatgagaaag gacagtcccg atatggtcaa
    13261 gagggttgta actggagatg aggccaggga cttggttgat gaaaggtcac ttctgatagt
    13321 tgttgacaac cacaagccaa gtctgacaga agctcccgag ttacttgaac tgacccagaa
    13381 ggttgtggtt atcgaccacc acagaagggg tgctgagttc ataaacaatc ctgtcctcac
    13441 ataccttgaa ccatacgcat catccacctc tgagcttgtt acggaggttc tgacatacat
    13501 gggcgaggag acgaacctga caaagtttga ggcagaggca ctccttgcag gaataactgt
    13561 agacacgaaa aacttcagct tccagacggg agtaagaacc tttgaggcag catctgtcct
    13621 taaaagggca ggagctgata ccacggttgt caagcagctt ttcagggacg acttcagcac
    13681 ctttgtcaac aaggccgagg tgattaaatc atcgaagatt gttttcgaca gaattgctat
    13741 aggaaggctt cagaataaga tggaggactc catattaata gccgcccagg ctgccaatga
    13801 gcttcttaac atcaatgacg tggaggcatc atttgtattg agccagctgg aggacaagat
    13861 ccacatttca ggacgttctc ttgggaacat aagcgtacag cttatccttg agaagctggg
    13921 gggaggaggg cacttgacca gtgccggagc ccaggtggaa aagctgtcaa tggacgaagc
    13981 tgaaaagctt ctaacagata taatagacaa atatttaaag gaaggtgaaa agtaatgaaa
    14041 gttatacttt tgaaggatgt caagggaatg gggaaggcag gtgaccttgt aaatgcaaag
    14101 gacggttatt caagaaacta ccttatccca agaaatattg caattgaagc aactgctgac
    14161 aaccttagga aatgggagga gtcaaaggcc aaggaggctg ccaataagga gcaggaaatg
    14221 aaggaagccc tggagcttaa ggaaaaaatc gagaagctta ctgtgaatat caaggcaaag
    14281 ggcggaaccg aaggtagact tttcgggtcg gtaacctcac aggacattgc agctgggctg
    14341 aaaaaacagc acaagattga tatagacaag agaaagatag aactgaagga caacataaag
    14401 acaaccggag taaccgaggt tgaagtgaag gtatatccag atgtatccgc tactttaaag
    14461 gtggatgtag ctgcagaata agaggtgaat cccatggaaa caggtattgg tagaatccct
    14521 ccacacagta tggaggcaga gcagtcggta ataggggcca tgcttcttga caaggaggca
    14581 ataaacacgg caatagaaca tcttcgggga gatgacttct acaaggaggc caacaaggag
    14641 atctatgagg ctatgcttgt tcttaacaac aggaatgagc ccgttgacct tgtaacactt
    14701 tcagaggagc ttaagagacg ggggtccctt gagggagtag gcggggtgac ctaccttgca
    14761 aacctttctg gaagtgtagc aactacagca aatacaaagt attattgcga catagtccag
    14821 gagaagtcaa tactaagaag actgattggg tccagcaatg aaataatggg ccttgcctac
    14881 gataattctg acagtgtcaa ttcaataatt gaaagagctg aaaagaacat attcgacatc
    14941 acccaggggg cccacaagca gagttttcat ccaataagcg aggtccttct ttcaagcttt
    15001 gcccagattg aggaaagggc tgccaacaag ggagagctga cgggtcttac tacaggattt
    15061 tcagaccttg accataagct ttcaggtcta cagaaatccg acctggtcct cctggctgcc
    15121 agaccatcca tgggaaagac ggcccttgga ataaacatag ccacaaatgc atccctaaag
    15181 ggaaatggaa aggttgcggt attctccctt gagatgtcca aggaacagct ggttcagagg
    15241 atcataagtg caacggcaca tgttgacctg cagaagataa taagcggtaa cctttccgac
    15301 gacgagtgga taaaggttgt aaacgccatg gcaccacttt ccaagatgga tatctacata
    15361 gacgacacgg cggggatatc actgatggaa atgaaggcca agtgccgtag gctcaaggtt
    15421 gagaagggtc tggacctgat agtcatagac taccttcagc ttatgcaggc tgatggacgg
    15481 gttgagaaca ggcagcagga aatctctgca atttcaaggg gcctgaaggc aattgccaag
    15541 gaaatggagt gcccggtcct tgccctttcc cagctttcac gtgctccaga gcttcgatcg
    15601 gaccacaggc ccatcctttc agacctgagg gagtcgggag ccattgagca ggatgcagac
    15661 gttgtaatgt tcctgtacag ggatgattat tacgaccagg agtcagagaa gaagaacgta
    15721 ggcgaggtaa taattgcaaa gcacagaaat ggtccaactg gacaggttga tcttgtgttc
    15781 aagcatgagt acaccaagtt tctcaatatg gaaaggtata ttgagccggg taatggttaa
    15841 agtccattga agggtcagac caaaccctga attgacacat tttaaagctt tgggaatcaa
    15901 cgaactagtc tttgattaac tgaggataat agtgtaaaat aacttcgagg ggagacctcg
    15961 aggttatttt tttggagatg tgaaaaagat gataataaaa ggaaaaaggg ttacactttc
    16021 ccccttgacc atggaagatg caaaatccat gaaaggctgg ggcgtccatg aaactgtact
    16081 tttgggagac tacaatttcc catttgaaaa tgacatagaa gtaaagagat ggttcagata
    16141 caagactttt acaatctgga acaagtactt tggcgtccac aatgaggaag ggaatcttgt
    16201 gggatacctg ggaataaaga acaaggactt catcaggaga agtgcatacc tgggaatagt
    16261 gtttgacccg gattacatgg acaggggtta tggaactgaa agtctggagg catttctacc
    16321 caagtatttc aatggtatga gaatgaagac cctttatctc gaggtggctg aattcaataa
    16381 aagggcctta agggtctatg aaaaacttgg ttttattcca atacagtact acacagacct
    16441 tttcccggtt gagggaattt ccaaggacca tcctgatata atggcttcaa aatcttgttt
    16501 tgtatttaaa gatgaaaaga tatataatta tatctacaaa atgaa
//
LOCUS       DASRQF010000132         4951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_443455, whole genome shotgun sequence.
ACCESSION   DASRQF010000132 DASRQF010000000
VERSION     DASRQF010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443455"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VFD79_03535"
     CDS             <1..774
                     /locus_tag="VFD79_03535"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZH93151.1"
                     /translation="EENAGALPMIEEGVLDNPKVDAAVGVHIWTPLPTGVVGISAGGV
                     MSGLYIFKIKVKGYGGHSGYPETAIDPVIAASDIVLSSQRIITREVSTLKPITMVFGQ
                     INGGTKANIIPDEVILEGSIRTLYDDSGDPPIYRLERLAKDVAKTHGCECEIEWIREN
                     IPLINDEKMTDHMKKTAIEVVGEDRVVPYRSMASEDFSEFTARIPGVFLFLGTGNEDL
                     GTHFPHHNPRFNVDESVLHLGVELFVRGSLKYLEDCSKN"
     gene            861..2006
                     /gene="dctP"
                     /locus_tag="VFD79_03540"
     CDS             861..2006
                     /gene="dctP"
                     /locus_tag="VFD79_03540"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HZH93152.1"
                     /translation="MKKSNKKLLLLLGLMLMGMVLLAGCGGGDTSAPAEEPAPAEEPA
                     ANGEPTEATLGDLITTPLQPRLASEEIEGDFMTVWAENYGDHMREVTDGKYDLDVYAY
                     GTLGDTRDINELAQLGVVEYVFSDYAWISSFVPEAQVLALHYIWPEERLPETLEWVVT
                     NGEFMNVLEEAFRRNGLVPLGVLYEGWQTITSNKPIETVSDMNGFKVRIMGSALLTQN
                     YLSYGADPTPMAYGEIYGGLQTNLIDGQINPIFAIKSMNFYEVQNYFTQIYAEPFLGI
                     PVVNMQYFDGLPAEVQEEMRNYWADAIIPAAEWIDARHEAELEEMLAARPEITYHEVR
                     GAELEEFKEAGRTVYDAFVGMGGPQGQEILDVLLADIENAKAELGIE"
     gene            2075..2746
                     /locus_tag="VFD79_03545"
     CDS             2075..2746
                     /locus_tag="VFD79_03545"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HZH93153.1"
                     /translation="MKDKGSSGLSLVYEKFERLVEHFEVFILSISVFAMASLVIVNVI
                     ARTFYRSIYFAEELTEVFTILITFTGVSYAVRKARHIRMGAFFDAVPYKIQKVMIFII
                     CGVSAVVMFLLSSYSYSYMDQARVMGHVTPALRMQYWYTLIIIPVGFFLAGVQYLRTI
                     IKNISEYDVWLSPEQQGEYEAEELAHIAEEYSSEGEQLSKKLYDGKDETGEDVSDDSE
                     DEFDT"
     gene            2762..4039
                     /locus_tag="VFD79_03550"
     CDS             2762..4039
                     /locus_tag="VFD79_03550"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HZH93154.1"
                     /translation="MLLLVSSMIFQLLFGFPFMVVLLGSLILYLVIYIPNFSMNVIIQ
                     QVVTGIIPPSLVAVPMFILAANIITSGQAAKRLIRMTKAFLGHIPGGLAITTNAACTM
                     FGAVSGSTQATVAAIGGTMRPMLLEAGYSSPFTLALIINASDIAFLIPPSIGFIVYGV
                     VTGTSVGKLFLAGVGPGLLIFLLFSIYAYIYSKRHNIVSPAKASREEKIDSVKSGILV
                     MGFPVIIIGGIYGGIFSPTEAAAASVAYALILEGLIYKTIRFSDMIDHFLQTGVVTGV
                     VFVLVGAGNAFSFLLSFLRIPQTYLPPLFGENPSQLYVIFIIVAAYFIACMFVDPIVA
                     IYVLSPIFAPYVLQTGVDPILLGVLVVLQAAIGSATPPFGCDIFTAQLIFRRPYLEII
                     SHTPPFIFILLLVTALLVAFPQIALFLPNAALI"
     gene            4069..4800
                     /locus_tag="VFD79_03555"
     CDS             4069..4800
                     /locus_tag="VFD79_03555"
                     /inference="COORDINATES: protein motif:HMM:NF005679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HZH93155.1"
                     /translation="MKYHRKKGQVCYGEAIGIILLDSEAPFIPGDVANATTYDFPVRF
                     QRVEGLTVQKMMDHDKSSLEKVMEAAELLKKEGVRAITSDCGFMALYQKDMREKLEIP
                     VFLSSLLQIPFIGEIIPYGRKIGIITANARSLTEDVIRMCGIEDVDNLEIAGLEDEPN
                     FSSAFLLEEGFVDTEKVEREVVDKALKLQKDNPDIGAILLECSVLPPYAEAAQRATGL
                     PVFDFVTMINYVFSAVVRESGGGFM"
BASE COUNT         1472 a          943 c         1226 g         1310 t
ORIGIN      
        1 gaagaaaatg caggagcttt acccatgatt gaggaaggag ttctggacaa tcccaaggtg
       61 gatgcagcgg tgggtgtaca tatatggacg ccattgccaa cgggggttgt tgggatatca
      121 gccggaggag taatgtccgg tctgtatata ttcaaaataa aggtcaaggg ctacggaggc
      181 cattctggtt atcctgaaac ggctattgac ccggtcatag cagcatcaga tattgtattg
      241 tcctctcaaa ggatcatcac ccgtgaagta agcacattaa agcccataac tatggtcttc
      301 ggacagatca acgggggaac caaggcaaat ataatacctg atgaggttat acttgaggga
      361 tccataagaa ccctttacga tgattctggg gaccctccca tctatcgttt ggagagactt
      421 gccaaggatg tggcaaagac tcatggatgc gaatgtgaaa tagaatggat cagggagaac
      481 attcctctga taaatgatga aaaaatgact gaccatatga aaaagactgc aattgaggtt
      541 gtaggtgaag acagggtagt tccatacaga agtatggcaa gtgaggattt ttccgagttt
      601 actgccagaa tacctggtgt ttttctcttc ctgggtacgg gaaatgagga cttgggaact
      661 catttccccc accacaatcc aagattcaat gttgatgaat cggtattgca tctgggtgtg
      721 gagttgtttg tcagagggag tctgaaatat ttggaagatt gtagcaagaa ttaacagaac
      781 attaccaata gtcgcaatat acccaaaaca taaaaaagac aaagtatgtt gtaatccaaa
      841 attcaaagga ggaaagtagg atgaaaaaaa gtaacaagaa gttactgttg ttgttaggtc
      901 taatgttaat ggggatggta ttactcgcag gatgtggtgg cggagataca tcagcgccag
      961 cagaggaacc ggcacctgcc gaagagcctg cagcaaacgg tgaaccaaca gaagctaccc
     1021 taggtgattt gatcaccaca ccacttcagc caaggctggc atcagaggaa atcgaagggg
     1081 atttcatgac cgtatgggct gagaactatg gggaccacat gagagaagta actgatggta
     1141 aatacgacct ggatgtttat gcatatggta ctcttggcga cacaagggat atcaacgagc
     1201 tggcacagct tggcgtagtt gagtacgtat tttccgatta cgcttggata agctcatttg
     1261 ttccagaggc acaggttctg gcacttcact atatctggcc agaggaaaga ctgcctgaga
     1321 cccttgagtg ggttgtaacg aacggtgagt tcatgaacgt tttggaagag gctttcagaa
     1381 gaaacggcct ggtaccattg ggagtgcttt acgaaggatg gcagacaatc acatcaaata
     1441 aaccaattga aacagtatct gacatgaatg gattcaaggt aagaatcatg ggatctgcac
     1501 tgttgacaca aaactacctt tcatacggag cagaccctac gccaatggca tacggtgaga
     1561 tctatggagg attacagaca aatcttatag atggacagat aaacccaata tttgcgatta
     1621 agagtatgaa tttctatgaa gttcaaaatt atttcacaca gatttatgct gagccattcc
     1681 ttggaattcc agttgtaaac atgcaatact ttgatggact tccagcagag gttcaagaag
     1741 agatgcgcaa ctactgggca gatgcaatta tacctgcagc tgagtggata gatgctcgtc
     1801 atgaagctga gcttgaggaa atgcttgctg caagacctga aatcacatac cacgaggtaa
     1861 gaggagctga gcttgaggag ttcaaggaag ccggaagaac agtatatgat gcctttgttg
     1921 gaatgggtgg acctcaagga caagagattc ttgatgtact tcttgcagac atcgaaaatg
     1981 ccaaggcaga gttagggatc gaataaccat atgaagaagt aagatttctg caaccaccca
     2041 ggtggttgca gaggttttag aaaggggtgt ggacatgaaa gacaaaggat catccggtct
     2101 ctcattggtc tatgagaagt ttgaaagact ggtagagcac tttgaggtat tcatattatc
     2161 tatatctgtc tttgcaatgg ccagtctggt tatagtgaat gttatagcca gaacctttta
     2221 caggagcatc tactttgcag aggaactgac tgaggtattc acaatactca taacctttac
     2281 aggagtaagt tatgctgttc gtaaagcaag gcacattcgt atgggagcat ttttcgatgc
     2341 agtcccatac aagatacaaa aggtaatgat attcataata tgcggtgtaa gtgcagttgt
     2401 gatgttcctg ctctccagct actcatattc atatatggac caggcaaggg taatgggaca
     2461 cgttactcca gccttaagga tgcagtactg gtatactctg attattattc ctgtagggtt
     2521 ctttctggca ggagtacagt accttcgtac cataatcaag aatatatctg aatatgatgt
     2581 ttggctttcg cctgagcagc agggagagta tgaggcagag gagctggctc acattgcaga
     2641 agaatacagc agcgaaggag aacagctgtc aaagaaactg tatgatggta aagacgaaac
     2701 tggagaggat gtttcagatg attccgaaga tgaatttgac acttaggggg gaggttgcca
     2761 tatgttacta ttagtatcat caatgatttt tcaattactg tttggctttc cctttatggt
     2821 tgtcctattg ggatcgctga tattatatct tgtgatatat ataccgaatt ttagcatgaa
     2881 cgtaattatc cagcaggtgg ttacaggaat tattccgcca tcactggttg cagttccaat
     2941 gtttattctg gccgcaaaca taatcacttc aggtcaggct gcaaagagac tgattagaat
     3001 gacaaaggcc tttcttggac atattcccgg gggacttgca ataacaacaa acgccgcctg
     3061 tacaatgttt ggagcggtat caggatcaac tcaggcaacg gtggcagcaa taggtggaac
     3121 catgagacca atgctccttg aggctggata ttcaagcccc ttcacccttg cactgataat
     3181 caatgcaagt gacatagcat tccttatacc accgagtatt ggattcatag tgtacggggt
     3241 tgttacagga acatctgttg gaaagctttt ccttgcagga gttggacctg gacttctaat
     3301 ctttttactg ttttccatat acgcatatat ctattcaaaa agacacaaca tagtaagtcc
     3361 tgcaaaggct agcagagaag agaagattga ctcagttaaa agcggcatac ttgttatggg
     3421 atttcctgta ataataatag gtggaatcta tggaggtatc ttcagtccta cagaggcagc
     3481 agcagcttca gttgcctacg cattgatcct tgaaggactt atctacaaga caatcaggtt
     3541 cagtgacatg attgaccact tcctacagac gggtgtagta acaggagtag tatttgtact
     3601 tgttggagcg ggtaacgcat tctcattcct gttgagcttt ctgagaatac cgcagaccta
     3661 tctaccacca ctgtttgggg agaacccatc acagctgtat gtaatattca taatcgtggc
     3721 ggcatacttc attgcatgta tgtttgttga cccaatcgtt gcaatatacg tacttagtcc
     3781 aatatttgca ccatatgtac ttcaaaccgg tgtagaccca atccttcttg gagtgcttgt
     3841 tgtactgcag gctgcaatag gatctgcaac accaccattt ggctgcgaca tatttacagc
     3901 acagctcata ttcaggcggc catatttgga aataataagc catacaccac ctttcatatt
     3961 tattctgttg cttgtaaccg cacttctggt tgcattccca cagatagctc tattcttgcc
     4021 aaacgccgct ctgatctagg gctgttggaa atataggagg tatatcgaat gaaatatcac
     4081 cgaaaaaagg gacaggtatg ctatggggag gcaattggaa ttattctcct tgatagcgag
     4141 gcacccttta tccctggaga tgttgcaaat gcaacaacat acgattttcc agtaaggttc
     4201 cagagggtag aggggcttac tgtccagaaa atgatggacc atgacaagtc cagtcttgaa
     4261 aaggtaatgg aagcagctga gctactgaaa aaagagggag tgagagccat aaccagcgac
     4321 tgcggtttta tggctcttta tcaaaaggat atgagggaga agctggaaat ccctgttttc
     4381 ctctccagtc tattacagat tccctttatt ggcgaaatca tcccctatgg aaggaagata
     4441 ggaataatca cagcaaatgc caggtccctg acggaggatg taatcaggat gtgtggcatt
     4501 gaggatgtgg ataacctgga aattgctgga cttgaggatg agccaaattt ctccagtgcc
     4561 ttccttcttg aagagggatt tgtcgatact gaaaaggtgg aaagggaggt ggtggacaag
     4621 gcccttaagc ttcagaagga taatcctgat attggagcaa tccttcttga gtgcagcgtc
     4681 ctgccaccat atgcagaggc cgcgcaaagg gccactggtc tgcctgtgtt tgactttgta
     4741 accatgatca actatgtatt ttcagcggtt gtcagagagt cgggcggagg ctttatgtag
     4801 gttgcaactt acaaattaag aggagggaat tttcagatgc atgtattcaa gaaagaggaa
     4861 taccaggaac gcattgcaaa gaccaagaag aaaatggagg agtggggaat agagctcttg
     4921 ttcgtttctc agcccgccaa catgaactat c
//
LOCUS       DASRQF010000133         2405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_15106, whole genome shotgun sequence.
ACCESSION   DASRQF010000133 DASRQF010000000
VERSION     DASRQF010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15106"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="VFD79_03560"
     CDS             complement(<1..1100)
                     /locus_tag="VFD79_03560"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZH93156.1"
                     /translation="MDRRERIESILKDYVGTVTHTGTELERNNQIFFEKWFKDQDYLE
                     RNPDNYGFHDIEGDHLGRKVPWCLLKGEGRDTIILIHHTDIVDTDAYGVYSSLAYKPD
                     EITQLYREGKVEISDEVKKDLDSGDWLFGRGVADMKGGASIHMALVEEYMKDKDFKGN
                     IILMGLPDEENLSAGMRGAVTLLEDLKGKHDLDYLLLLNVEPHDRNNPDVQTLYDGSV
                     GKLMPVVYVRGALTHVGHIFQGLNPINLLSEIVRKTEVNPDFLEKVGNTVTPPPTWLY
                     MKDGKNVYDVSLPMSAAGYMSILTLAKPPKDIFEALRKTSVEAFEAVIEELNISYSKY
                     LELKGEDQKKLPWKTNVKLYGELYQQAIEDSG"
     gene            complement(1167..1535)
                     /locus_tag="VFD79_03565"
     CDS             complement(1167..1535)
                     /locus_tag="VFD79_03565"
                     /inference="COORDINATES: protein motif:HMM:NF028342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZH93157.1"
                     /translation="MIFIAGVYPRQKKLEYGGGPEVCKSCGRYGSYQVYMEYNTFSLF
                     FIPIIKWKKRYFVVTSCCGERLQLDPEIGERIERGDNIRIGEEDLIRQEGFGGISYCN
                     GCGQPLDPEFSYCPNCGKKQ"
     gene            complement(1665..>2405)
                     /locus_tag="VFD79_03570"
     CDS             complement(1665..>2405)
                     /locus_tag="VFD79_03570"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZH93158.1"
                     /translation="VSGLIYSLLLSFASINMKANQVIVGTALNMLIPAVILLFSKMFF
                     NSDGITTSMRFYIREIPVLGQIPIIGPMFFQRTYLTVIIGFIILAIAVILLYKTRFGL
                     RLRACGEHPHAADSVGINVYKMRYAGVSLSGILAGIGGFFYAVGVMDGNVSGHTGVAG
                     FGFLALAVMIFGQWKPIRIMFAAMFFAFLRTLAYSVSLIPFLFNLGIDQTYYKILPYL
                     ATMVVLALTSKNSRAPKAEGIPYDKSQR"
BASE COUNT          641 a          595 c          451 g          718 t
ORIGIN      
        1 tcccccgaat cctctatggc ctgctggtaa agctcaccat aaagcttcac atttgtcttc
       61 cagggtagtt ttttctggtc ttccccctta agttcaagat atttggagta gctgatgtta
      121 agctcctcta tgacagcttc aaaggcttca acagatgttt ttcgcagggc ttcgaatata
      181 tcctttggcg gctttgccag tgtcaggatg ctcatgtagc cagctgccga cattggaagg
      241 cttacatcgt atacattttt cccatccttc atgtatagcc atgttggagg aggtgtaaca
      301 gtattcccta ccttttcaag aaagtctgga tttacctctg tttttctgac tatctctgaa
      361 agcaggttga ttggattaag gccttggaaa atgtggccta catgtgtcaa ggctcccctg
      421 acatatacca ctggcataag ctttccaact gacccgtcat acaaggtctg aacatccgga
      481 ttgtttctgt catggggttc aacattgaga agaagaaggt agtccaggtc gtgctttccc
      541 ttcaggtctt caaggagagt aacagctccc ctcattccag ctgaaagatt ctcctcatca
      601 ggaagtccca tcagaatgat gtttcccttg aagtctttat ccttcatgta ctcctcaacc
      661 aatgccatat ggattgaagc accacccttc atatcggcca cgcctctacc aaaaagccag
      721 tctccactat ccaggtcctt cttcacctca tcagagatct caaccttccc ctccctgtac
      781 agctgtgtaa tttcatcagg cttgtaggca aggctgctgt aaactccata ggcatcagta
      841 tcaacaatgt ctgtatggtg aatcaaaatt attgtatctc ttccttctcc cttcaggaga
      901 caccagggaa ccttccttcc aagatggtct ccttcgatgt catgaaatcc gtagttgtct
      961 ggatttcttt ctagatagtc ctggtcttta aaccatttct cgaaaaagat ttggttattt
     1021 ctctctagct ctgttccagt gtgggttacg gtcccaacat aatccttaag tatgctttca
     1081 attctttctc ttctatccat tttatcactc ccaatatcga tttctcatca cattgtatca
     1141 gtaaagcaat aaattcacaa taaccatcac tgctttttac cacagtttgg gcagtaagaa
     1201 aattcagggt ccaagggctg accgcatccg ttacaatagc tgattcctcc aaagccttcc
     1261 tgccgtatca aatcctcctc gccaatacgg atattgtccc ctctctcgat tctctctcct
     1321 atctccggat caagctgcaa cctctctcca cagcaggaag taacaacaaa atatctcttc
     1381 ttccacttta ttattggtat gaaaaagaga ctgaaggtgt tgtattccat gtaaacctgg
     1441 tagcttccat accttccgca ggacttgcac acctccggtc ctcctccata ttcaagcttt
     1501 ttttgtcttg gatagactcc cgcaataaat atcatagact caatcccctc tataatctgg
     1561 tataggatta ttatacatta ctttggaact acagaaaagt aaacaagccg aaatatagtc
     1621 cctggcgctt ccaccaggga ctgactataa ttctatgaga ttctttatct ttgcgacttg
     1681 tcgtagggga ttccttcagc cttgggtgcc cttgagttct tggatgtaag tgccaggacc
     1741 accattgttg caagatatgg caagatttta tagtaggtct ggtcgattcc aagattaaag
     1801 aggaatggaa taagtgacac tgaatatgcc agtgttctca ggaaggcaaa gaacattgcc
     1861 gcaaacatta tcctgattgg cttccactgc ccaaatatca taactgccaa tgcaaggaat
     1921 ccaaaacctg caacccctgt gtgaccactg acatttccat ccattacacc aactgcgtag
     1981 aagaagcctc ctattccagc cagtatccct gacaggctta ctccagcata tctcattttg
     2041 tagacgttaa ttccaactga gtcagctgca tgagggtgct caccgcaggc tctaagtcta
     2101 agaccaaatc gggtcttgta aagcagtatg accgctatgg caagaattat gaagcctatt
     2161 ataacagtaa ggtaggtcct ttgaaagaac attggaccaa ttattggaat ctgacccaat
     2221 acgggaatct ctcttatata gaacctcatg cttgtcgtta taccatcact gttgaagaac
     2281 atctttgaaa atagaagtat cacagccggt ataagcatat ttagcgcggt tccaacgatt
     2341 acctggtttg ccttcatgtt tatggatgca aaggaaagca agagggaata aatcagtcca
     2401 gatac
//
LOCUS       DASRQF010000134         4580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_890252, whole genome shotgun sequence.
ACCESSION   DASRQF010000134 DASRQF010000000
VERSION     DASRQF010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890252"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1612
                     /gene="glpK"
                     /locus_tag="VFD79_03575"
     CDS             113..1612
                     /gene="glpK"
                     /locus_tag="VFD79_03575"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HZH93159.1"
                     /translation="MTRKFILALDQGTTSSRAILFDKQGEIYGIAQKEFKQYYPQEAW
                     VEHDPMEIWGTQSGVIREVMESASVTPDEIAAIGITNQRETTIVWDRETGRPVYNAIV
                     WQCRRTAKYCEDLKERGLSEYVRKNTGLLIDAYFSGTKIQWILDNVKGARERAERGDL
                     LFGTVDTWLLWNLTGGKVHKTDYSNASRTMLYNIRNLQWDRKLLGELNIPEAMLPQVM
                     DSSGIFGYTDEKSFGARIPIGGIAGDQQSALFGHACFEKGMVKNTYGTGCFMLMNTGE
                     EPIESGSGLITTIAWGLEGKVTYALEGSIFVAGAVVQWLRDGLKLIRSAAESEDLARR
                     VDDTNGVFMVPAFVGIGAPHWDMYARGTIVGMTRDTTGDHIVRAALESIAYQTRDVLE
                     AMEKDSGISLKTLRVDGGAVENNFLMQFQSDILNLDVERPVTRETTALGAAYLAGLAV
                     GYWSDRDEIIKNWNLDRKYSPLMDELKRGQLYSQWKKAVGRSLAWKGEQ"
     gene            1612..2211
                     /locus_tag="VFD79_03580"
     CDS             1612..2211
                     /locus_tag="VFD79_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HZH93160.1"
                     /translation="MPNIDGIILAAGFSRRMGKDKLLLPFGEDLVVERVVKTALKSRL
                     SKVILVYRNEEVAEIGKKYGVETIKNTRARLGQAESVKSGLEKATGDGYLFIAGDQPF
                     LSCSLVNGLIRAFASSGKGIVIPTFEGEVHMPILFSNKYRKDLEKVRGEKGGFEIISG
                     NKEDIYWYHLEDQLDVCDIDTETQYMELIHRKDAKDNGL"
     gene            2201..2983
                     /locus_tag="VFD79_03585"
     CDS             2201..2983
                     /locus_tag="VFD79_03585"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HZH93161.1"
                     /translation="MDYRIEKKKGKRFLYLPELQEMGLRHCFTTRDMDVGSSTNPDRD
                     LLQSHIHEAYDFMGLKPRLLYNGYQAHTGNIAVIRSLEEGKEGAFGRFIPNTDGLLTD
                     MEEVALLTRFADCVPVILFDQVKRVQGNIHSGWKGTLERIVQNGIRGMVKEYGSSLED
                     IVVVIGPAIGRDDFLVREDVSSLFTGEFPGWKDLVRRVSKDQFLVDLQEINKRLLLEA
                     GIMEENITVVNISTFGRDDLCHSYRRDRDKYGLMGLFTVLDE"
     gene            3366..4547
                     /locus_tag="VFD79_03590"
     CDS             3366..4547
                     /locus_tag="VFD79_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93162.1"
                     /translation="MSLMMAFSTVLIVLALGDIVSTKTKAFVPSVFVAALLFLFGFWT
                     FFPEDIVALAGFQMPVVTLSMYLLITHMGTMMSVRELAGQWRTILIALIGIIGIMTLT
                     MTIGAAVFGRETAIIATPPLTGGIVAAIVMSDAATAIGRDNLAILAIITYVMQGFVGY
                     PLTSIMLKKEGTRLVNGFRSGEIKHVKEEVVVNKPVRKKLIPQVPKDYQTTYLTLARL
                     GLTAWAAVGFAGLIAPIINISAFVMCLLFGVIAQEIGFVDQKPLNTSSSFGFLITVLM
                     SYVFAGLARATPAMLMEIVVPLMGIIILGVSGMAILSMVLGKRLGYTKEMAFAVALTA
                     LYGFPPNYILTEEASKALGETPEEREFLMDEMLPKMLVGGFTTVTIVSVIIAGIFVNL
                     I"
BASE COUNT         1414 a          837 c         1174 g         1155 t
ORIGIN      
        1 ggttaggagg aagcctgaaa gggcttcttt ctttttgtgg aaaaaacaaa ggaaactcca
       61 aaaaagggga aattattgat ataattaaca aaagggcaaa ggggtgagtg atttgaccag
      121 gaagtttata ctggcactgg accagggtac aaccagctcc cgtgcaatcc tgtttgacaa
      181 gcaaggtgaa atctatggga ttgcacaaaa ggaattcaag caatactatc cccaggaagc
      241 atgggtggag catgacccca tggaaatatg gggaactcag tcgggcgtaa taagggaggt
      301 tatggaaagt gcttcagtaa cccccgatga aatagccgct atagggataa ctaatcaaag
      361 agaaaccaca atagtctggg atagggaaac agggagacct gtgtacaatg ccatagtatg
      421 gcagtgcaga aggactgcca aatactgcga agaccttaag gaaagaggcc tatcagagta
      481 tgttagaaag aatacaggac tcctcatcga tgcatatttt tcagggacca agattcaatg
      541 gattcttgac aatgttaagg gggccaggga aagggccgag agaggagacc tgctttttgg
      601 aacagttgac acctggcttc tatggaatct tacaggtggg aaagtccaca agacagatta
      661 ttcaaacgca tcaaggacaa tgctctacaa cattaggaat cttcagtggg acaggaagct
      721 cctaggagag ctgaatattc cagaggccat gcttccccag gtaatggact catctggaat
      781 attcggctat acggatgaaa aaagctttgg tgccaggatt ccaattgggg gaattgcagg
      841 agaccagcaa tctgccctct ttgggcatgc ctgctttgaa aagggcatgg tcaagaacac
      901 ctacggaaca ggctgcttta tgctaatgaa cacgggagaa gagccaatcg aatcgggaag
      961 tggccttatc acaaccatag catggggact tgaaggcaag gttacctacg ccctggaggg
     1021 gagcatattt gttgcaggtg cggtggtgca gtggctaagg gatggactta agctgatacg
     1081 gtctgcggct gagtccgaag acttggcaag gagggtggat gacaccaatg gggtttttat
     1141 ggttcctgca tttgtgggaa ttggagcacc acattgggat atgtacgcaa gaggcacaat
     1201 tgtggggatg acaagggaca ctacaggaga ccatatagta agggcggcct tggagtcaat
     1261 cgcatatcag acaagggatg ttttggaagc catggaaaag gactcgggta tatccctaaa
     1321 aaccctcagg gtggatggtg gagcagttga aaacaacttt cttatgcagt ttcagtcaga
     1381 tatcttgaac ctggatgtgg agaggcctgt tacaagggag accacagccc ttggtgcagc
     1441 atatctggct ggtcttgcgg taggatactg gtctgacagg gacgagataa tcaaaaactg
     1501 gaatctggat agaaaatact cacccctgat ggatgaactc aaacgtggtc aactgtacag
     1561 tcagtggaaa aaggctgttg gaagatccct tgcatggaaa ggagaacaat gatgccaaat
     1621 atagacggaa taatccttgc cgccggcttc tccagacgga tgggcaagga taagcttctc
     1681 cttccatttg gggaggacct ggtggttgag agggtggtca agactgccct taagtccaga
     1741 ctttccaaag tgatacttgt atacaggaat gaggaggtag ctgaaatcgg aaaaaagtat
     1801 ggggttgaaa ccataaaaaa taccagagcc cgtctgggac aggctgaaag tgtcaaaagc
     1861 ggactggaga aggcaacagg tgatggctac ctctttattg ctggcgacca gccatttctg
     1921 tcctgctctc tggtaaatgg cttgatccgt gcatttgcaa gctcgggtaa aggtattgta
     1981 atcccaacct ttgagggtga agttcatatg cccatattat tttccaacaa atatcgcaag
     2041 gacctggaga aggttcgcgg ggaaaagggt ggctttgaga taataagtgg aaacaaggag
     2101 gacatctact ggtatcacct tgaggaccag ctggatgtat gtgacattga cacagagact
     2161 caatacatgg aattaattca caggaaggat gcaaaagaca atggactata gaattgaaaa
     2221 gaaaaagggc aagagattct tgtacctgcc agagctacag gaaatgggac ttaggcattg
     2281 cttcaccacc agggacatgg atgtgggctc aagtacaaat cctgacaggg accttctaca
     2341 gagccatatc catgaagcct atgactttat gggtctaaag cccagacttc tctataatgg
     2401 ctaccaggcc cacacaggaa acatcgcagt tataagatcc cttgaggaag ggaaggaagg
     2461 tgcttttgga aggttcattc caaataccga tgggcttctt acagatatgg aggaagtggc
     2521 cctccttaca agatttgccg actgcgtacc ggttattttg tttgaccagg ttaaaagagt
     2581 ccagggcaac attcattcag gctggaaggg aacccttgaa agaatcgtac aaaacggaat
     2641 aaggggtatg gtaaaggaat acggatcatc tctggaggac attgtcgttg taataggtcc
     2701 agccatagga agggatgatt ttcttgttag ggaggatgta agcagccttt tcacggggga
     2761 attcccggga tggaaggatc ttgtgagacg ggtttcaaag gaccagtttc tggttgatct
     2821 tcaggaaata aacaagagat tgcttctgga ggctggaata atggaagaaa acataaccgt
     2881 cgttaatatt tcaacctttg ggcgggatga cctctgccac tcatatcgaa gagacaggga
     2941 taagtatgga ttaatgggac tttttacggt ccttgatgaa taaacaacat agaaagagct
     3001 tccctttggg gaagctcttt ctatgttgtt tgtacctatc tatgtagagg ggtctttcca
     3061 atttgagtcc agtaaatgca aagagggaaa ggatttactt tagaagctcg aaagaccaag
     3121 aaaaaagcaa aagtgataaa aatataacaa aacatatcaa aaaatttaca tttattaatc
     3181 tttataaatc aattataaaa gataaaatga gaaaatgcaa gggaaataag agaaaattga
     3241 taaaaaaata tctaacaatc agccccgata cttcaaataa tacttagtta ttttgttttt
     3301 gacaccatat acattgcaaa tatttaatgg tatactgtgc taaataaata tggaggagga
     3361 attatatgag tttaatgatg gctttttcaa cagtattgat cgtacttgct ttgggagata
     3421 tagtttccac gaaaacaaag gcattcgtac cgtcagtttt cgtagcggca ctcttgtttt
     3481 tatttggttt ctggacattc ttcccagagg acatcgtagc cctggcagga ttccaaatgc
     3541 ctgtggtaac cttgtcaatg taccttttga taacacatat gggtacaatg atgtcagtaa
     3601 gggagcttgc aggacagtgg agaacaattc taattgcact tatagggata attggtataa
     3661 tgacccttac aatgacaatc ggagcggctg tgtttggaag agaaacagca ataatagcaa
     3721 ctccaccact aactggtgga attgtagcag caatagtcat gagtgacgct gcaacagcaa
     3781 taggaagaga caaccttgca attcttgcaa tcataaccta tgttatgcag ggatttgtag
     3841 gctatccctt gacttccata atgcttaaga aggaaggaac taggctggtt aatggcttca
     3901 gaagcggaga aataaagcat gtgaaggaag aggtggttgt gaacaagcct gttagaaaga
     3961 agcttatacc acaggttcca aaagactacc agactaccta cctgactctg gcaaggcttg
     4021 gtttgactgc ctgggcggcg gttggttttg ctgggctcat agcacctatc ataaacattt
     4081 cagcctttgt aatgtgcctg ctatttggag ttattgcaca ggaaatcggg tttgtggatc
     4141 agaagccact taatacatca agctcatttg gattccttat aactgtactt atgtcctacg
     4201 tatttgcagg acttgcaaga gcaacccctg caatgctaat ggagatagtg gttcctctta
     4261 tgggaataat cattcttggt gtttcaggta tggcaattct ttcaatggtt cttggaaaga
     4321 gattgggcta taccaaggag atggcatttg ccgttgcatt gactgcgctt tatggtttcc
     4381 caccaaacta cattcttaca gaagaggcat caaaggcact tggtgaaacc cctgaggaaa
     4441 gagaattcct tatggatgaa atgcttccaa agatgcttgt tggaggattt acaacagtaa
     4501 caatcgtatc cgtaataatt gcgggaattt tcgtaaatct gatttagcaa aaagcgcctt
     4561 ttcaggcgct ttttatacaa
//
LOCUS       DASRQF010000135         3731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1502998, whole genome shotgun sequence.
ACCESSION   DASRQF010000135 DASRQF010000000
VERSION     DASRQF010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502998"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VFD79_03595"
     CDS             <1..384
                     /locus_tag="VFD79_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93163.1"
                     /translation="IEAEGYKVNTIALPYGTRPKDESKTSYLKEGTYDGVPYDNIAIL
                     NVGWNPGYSPYDERFNPESIPRIRASEMKVDNVGMYNYLEYFDNNPDQRFISDGNPDI
                     ITVPDDRAEQIGSQQDREIYIYDKK"
     gene            404..2155
                     /locus_tag="VFD79_03600"
     CDS             404..2155
                     /locus_tag="VFD79_03600"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HZH93164.1"
                     /translation="MKNRSLKYILTLLILSLMIGCAQGPQEPTEQPQESEEAVEAEEV
                     PEVEEISEEEKMQIRIQEREDLEKNRKAEMGEFYVPLPPLDVDNELKTVKAKALYLTG
                     NVADFQFQQEDIDYYADYVLALSGQSGETPDPSRLGDVNKLEIALGIAKASEINAMVI
                     DIKNDHGLVTWESDLEIVNKVESSINPPFKNYMPLMDYLEENEIYTIARVVAFKDPIF
                     ASANPAHAILLKEGGVYLDNAGESWVNPFDQYVWDYVVAVSKEAALRGFDEIQYDYVR
                     FPDNARYYNPITEFPGRDGRDKDEGIEDFLEYASGELEPYNVHLSADVFGFITHSWDD
                     KPEDIGQTWRKIANKVEYICPMIYPSHYGPGVYGFDVPDRYPYEVARISVMEAIERNA
                     AQKEPAIIRPWFQGFTASWVGGNIKYGPEEISDQMVAAAELGVDEYIIWSAGNNYNPM
                     SFFYHDRIDESIVKEGHDIMDRTPEMALDRFLKSERSTRLSQLYLLTPIQERPEDYDR
                     FVEDYEGINPVLQRFKVSEVVERDDGSYLAKVDVEYKSDLGTFALDNAEYVIREENRV
                     FKISRPEIEWVEDSTEE"
     gene            complement(2208..2573)
                     /locus_tag="VFD79_03605"
                     /pseudo
     CDS             complement(2208..2573)
                     /locus_tag="VFD79_03605"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
     gene            complement(2589..>3731)
                     /locus_tag="VFD79_03610"
     CDS             complement(2589..>3731)
                     /locus_tag="VFD79_03610"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HZH93165.1"
                     /translation="SVIVMTKLMMITAVLSGFMNNIGALALVLPVAIKVAREHRFSPS
                     RILMPLAFSALLGGMTTEIGTPPNLIISSYRQEYGLESFGFFDFTPVGLTVTVLGIGL
                     MVILGGKLIPKRKTIGVERLFDIGEFLSEVQVVESSKIAGQPMADINRVFKLDIDVLS
                     ILRNEKKIVAPGPDEILVPGDILIIKALSEDLADLIDKTQVLLRGTKKSLHVEESMIK
                     SRDTALVEVVLRDDSPIIGRTALETKLRNRYSINLVAVSRKGVYSVDRLKSMRFRQGD
                     ILLIQAPTSILKDIYQKLGCLPLAERGVEIYPDRAKWKLAFTAGIFIFSVVLTTTGLL
                     PVQTSFSFAATALVLFRILTPREFYDAIEWPSIIMLGSLIPLGSAL"
BASE COUNT         1167 a          776 c          876 g          912 t
ORIGIN      
        1 attgaggctg aaggatacaa ggttaacaca atcgccctcc cctacggcac aagaccaaag
       61 gatgagtcca agacaagcta tctcaaggaa ggtacctatg atggagttcc ctatgacaac
      121 attgcaatcc tcaatgtagg atggaatcct ggctactcac cctatgatga aagattcaac
      181 ccggagagca ttccaaggat acgggccagt gaaatgaagg tggacaatgt tgggatgtac
      241 aactatctgg agtattttga caataaccct gaccaacgat tcataagcga tggcaatcct
      301 gacataataa ctgtgccaga tgatagagcc gaacaaatag gctcgcaaca agacagggaa
      361 atatatatct atgacaaaaa ataagacatg aagaggtgga caattgaaaa atagaagctt
      421 gaaatatatt ttaaccctac tgatcctttc tctaatgata ggctgtgccc aaggaccaca
      481 ggagccgact gaacagccac aggaatctga ggaggccgtt gaagctgaag aagtaccaga
      541 ggtggaggaa ataagcgaag aagaaaaaat gcagattcgc atccaggaaa gggaagacct
      601 tgaaaagaac aggaaagctg aaatgggaga gttttatgtt ccattacctc ccctggatgt
      661 agacaatgaa ctgaagactg taaaggcaaa agccctctac cttacaggaa atgttgctga
      721 ttttcagttt caacaggaag acatcgacta ctatgctgac tacgtattgg ccttaagtgg
      781 acaatcggga gagacgccag acccttcaag gcttggggat gttaacaagc tggagattgc
      841 actgggtata gccaaggcat cagaaatcaa tgccatggta attgatataa aaaatgacca
      901 tggtttggtc acatgggaaa gcgaccttga aattgtcaac aaagtagaat ccagcataaa
      961 tccacccttc aagaattata tgccccttat ggactacctg gaggagaatg aaatatatac
     1021 aattgctaga gttgttgcct ttaaggaccc aatctttgca tctgccaatc cagcacatgc
     1081 aatccttcta aaggagggcg gcgtatatct tgataatgca ggggagtcat gggtaaatcc
     1141 ctttgaccaa tatgtttggg actatgtagt agcggtatca aaggaggcag cactgagagg
     1201 atttgatgaa atccaatacg actatgtcag atttcctgat aatgcaagat attacaaccc
     1261 cataactgaa ttccctggga gggatggaag ggacaaggat gaggggatag aagacttcct
     1321 ggaatatgcc agtggagagc ttgagccata caatgttcat ctctcagcag atgtctttgg
     1381 attcataacc cattcctggg atgacaagcc tgaggatatt ggccagacct ggagaaagat
     1441 tgccaacaag gtagagtata tttgccccat gatctaccca agccattatg gaccaggggt
     1501 ttatggattt gatgtccctg acaggtatcc atatgaggtt gccagaattt ctgtcatgga
     1561 ggcaattgaa agaaacgctg cacagaagga gcctgccata atcagaccct ggtttcaagg
     1621 ctttactgct tcttgggtgg gaggaaatat taagtacggc cccgaggaaa tttcagacca
     1681 aatggtagca gccgcagagc ttggtgttga tgaatacatc atttggagcg cagggaacaa
     1741 ctataatcct atgagctttt tctaccatga cagaatcgac gagtccattg taaaggaagg
     1801 ccatgacata atggacagaa ctccggaaat ggctcttgat agatttctta agtcagaaag
     1861 gagcaccaga ctttcacagc tataccttct cacaccgata caggagcgac cagaggatta
     1921 cgataggttt gtcgaggatt atgaagggat caatcccgtg ctccaaagat ttaaggtcag
     1981 tgaggtagtt gaaagagacg atggtagcta tttggcaaag gttgatgttg aatacaaatc
     2041 tgatttggga acatttgctc tggataatgc agaatatgtg atcagggaag aaaacagggt
     2101 gtttaagata agcaggccag agattgagtg ggttgaagat agcacggaag aatagattag
     2161 aaaaaggatg tggcaactaa gttttaagtt gccacatcct ttttagatta catgggccac
     2221 actagaagta tcagcggtgc gccgacgacc acaagcagta ttgtgagggg aagcccaaga
     2281 taccagtagt ctgtaaactt gtaacctcct ggccccataa ccagcatatt ggtctggtgc
     2341 ccgattgggg tcaggaatgc agctgaagct gaaataacca cgctcataag gtaagggtca
     2401 ggtgagacac ctgttgtaat cgcaatactc agtgcgattg gacccattag aattgcagtg
     2461 gctgaagtgc ttatgaggtt ggctagagcc aatgtcaaaa tcatcaagag accaatcata
     2521 agtgtcggtg aaagaactgt agatgccctt agaagtatat tcgcaattgt atccgcacct
     2581 ccgctgcttt aaagggcgct accaaggggg atcagtgaac caagcattat gattgatggc
     2641 cattcgatag catcgtaaaa ctctcttgga gtcaggattc taaaaagtac aagggcagtg
     2701 gcagcaaagg aaaatgatgt ctgaaccggc aataggccag tggttgtcaa gacaacggaa
     2761 aagataaaga ttcctgcagt gaaggccagt ttccattttg ccctgtcagg atagatttca
     2821 acgcctcttt ctgcaagggg tagacagccc aacttttgat aaatatcctt taggattgag
     2881 gtgggtgctt gaatcagaag aatatcaccc tgacgaaacc gcatgctttt caacctatca
     2941 acagagtaga caccttttcg tgatactgca accaaattga tactatacct gttcctaagc
     3001 tttgtctcca gggcggttcg tccaataatt ggagaatcat ccctaaggac aacttcaaca
     3061 agtgctgtat ctcttgattt gatcattgat tcctccacgt ggagactctt ttttgtaccc
     3121 ctcaacaaaa cctgggtctt gtcgattaga tctgcaagat cctcagacaa ggccttgatg
     3181 atcaatatgt cccctggaac tagaatctca tctggccctg gtgcaactat cttcttttca
     3241 tttctcaaga tactcaacac atctatatca agcttgaaga ccctgttaat gtctgccata
     3301 ggctgccccg caatcttact ggattcaacc acctggactt cagacagaaa ctcaccaata
     3361 tcaaaaagcc tctcaacacc tattgttttt ctctttggta tgagctttcc accaagaatt
     3421 accatcaaac ctattccaag aaccgtcacc gtcaagccta caggtgtgaa gtcgaaaaaa
     3481 ccaaaggatt caagtccata ctcctggcga taggatgata ttattaggtt gggcggtgta
     3541 cctatctctg ttgtcatccc acctaggaga gctgaaaaag caagaggcat cagtatcctc
     3601 gaaggcgaaa acctatgttc acgtgcaacc tttattgcca ctggaagaac cagggcaagg
     3661 gccccgatgt tgttcataaa cccggaaaga actgctgtaa tcatcatcaa ctttgtcatt
     3721 acaatgacgg a
//
LOCUS       DASRQF010000136         3757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1648348, whole genome shotgun sequence.
ACCESSION   DASRQF010000136 DASRQF010000000
VERSION     DASRQF010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1648348"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VFD79_03615"
     CDS             <1..188
                     /locus_tag="VFD79_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93166.1"
                     /translation="ALSLIATINNEYFYGSSMMGEVFMGAKMRLTRNGIDIPSYDLHP
                     LLKTRLEKTYNQLRSMI"
     gene            239..1228
                     /locus_tag="VFD79_03620"
     CDS             239..1228
                     /locus_tag="VFD79_03620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZH93167.1"
                     /translation="MIDRAVNGENPNIVKNDEDLPDLRGHNILFAVELNSIGINPNLN
                     RILESMASKGKNALEKARASILIHSSYDNFTKTAAQDIVFIANQLGCTFPGRPVIEAN
                     QNLDNYIPLRKIYDLPLEEICLQKSEELGRRLLSTQQYFRNKTIAVLHSSNKGTSNTY
                     SLWEMVKSKLEDKVHINEVYLGNGTITDCRGCSYKTCKYFGKQAKCFYGGVVVEEVYP
                     AIQDASTVILLCPNYNDMVTANIVATINRLTALFRKTKFYDKKIFTVIVSGYSGGDAL
                     AKQLISSLNMNKTFELPPNFSIMATANDPGAIHKVPGIRTKAESFANHILENL"
     regulatory      complement(1250..1368)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(1373..1558)
                     /locus_tag="VFD79_03625"
     CDS             complement(1373..1558)
                     /locus_tag="VFD79_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HZH93168.1"
                     /translation="MGFGRLGAPELILILAIALVIFGPSKLPEIGKSLGKAIGEFKGH
                     ANKVTEDLNVDLKEEKK"
     gene            complement(1610..2335)
                     /gene="fdhD"
                     /locus_tag="VFD79_03630"
     CDS             complement(1610..2335)
                     /gene="fdhD"
                     /locus_tag="VFD79_03630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HZH93169.1"
                     /translation="MLDMINAIELSEEFPVTINLNGEKLVTLMCTPTDLRELAIGHLI
                     NTGVVNSFSDIMTVAACEDLRQIYVMAENVTDRRYTLGDIYGTGCGSTLDFPEEISQL
                     AIGECDVLLNFRRIKTAFERMYQDAEKYKRHGGIHASAILYKKDIVTMEDVARHNTHD
                     KVVGYAAYRGWDLSKSTIITTGRISTDMVLKAARAGVPIICSRSNPTSLAVDLGFKLG
                     ITLVGRAASGNATVFTHKDRIMM"
     gene            complement(2351..2902)
                     /locus_tag="VFD79_03635"
     CDS             complement(2351..2902)
                     /locus_tag="VFD79_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93170.1"
                     /translation="MDEKMKYMQEDVADFIVRNSRDGKIVTLAEIEASLVYGDEDQAT
                     DISQILESIGTDLDLKKIDDAEGNTFYYSGNEMTDKYAGIIIRVKDKDILKLIGDTVR
                     EESRRYPRPTYVTMFNLPPYNLTEGELTDMLSELSNDSEYADLKFTNASNGTLFVYSN
                     KHMSKGYADYLAEWIEVGKDENP"
     gene            complement(2914..>3757)
                     /locus_tag="VFD79_03640"
     CDS             complement(2914..>3757)
                     /locus_tag="VFD79_03640"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HZH93171.1"
                     /translation="FLKDFYRRITFKEGEFSHLGDGTYWIAKRWNFGDDFWHGKDYKI
                     WSPLGYPVHHSNEARAQVGALISCMFNRDSQSHTHMNLIDSGLPADILREVSAEIWGS
                     EEAFDFPAKYTPMNEYKAKFAKWSIIRNVLHDSLTVCNWMWPMMVSPVKERNYRGDTA
                     LESKYMSIVTGENYTEESLDLAAERVFTLHRALTVKQMGTVDMRNEHDVIMDWVYDMD
                     PDMEPFTEGTVKMEREDMQTALTMFYEEMGWDKKTGAPTKETLKRLGLDFAASELESM
                     NLLP"
BASE COUNT         1085 a          888 c          656 g         1128 t
ORIGIN      
        1 gagctttgtc acttatagcc accataaata atgaatattt ttatgggtct tccatgatgg
       61 gagaggtttt tatgggtgct aagatgcgac taacaaggaa tggcattgat ataccttcct
      121 acgatctaca ccccttgcta aaaaccagac ttgagaaaac ctataaccaa ttaaggagta
      181 tgatatgaga ccatttacgt taattttgcc ttcacaacca tccccactgc tccaaaaaat
      241 gatcgaccgg gcagtgaacg gtgaaaatcc aaatattgta aagaacgatg aagatttgcc
      301 agatttaaga ggacacaaca ttctatttgc agtggaactg aactccattg gaataaatcc
      361 aaaccttaac agaattcttg aaagtatggc aagcaagggc aaaaatgctc ttgagaaggc
      421 tagagcctca atactgatac acagtagcta cgataatttc acaaaaaccg ctgcccagga
      481 tatagtcttt atagcaaatc agctaggctg taccttccct ggaagaccgg ttatagaagc
      541 aaaccagaat cttgataatt atataccttt aagaaagatt tacgatttac ccttggagga
      601 gatttgcctt caaaaaagtg aagagcttgg tagaagactt ctctcaaccc aacagtactt
      661 cagaaacaaa acgattgctg tgctccactc ttcaaacaaa ggaacctcga atacctactc
      721 cctttgggaa atggtaaagt caaagttgga ggacaaggtt cacataaatg aagtatacct
      781 tggaaacggt acaataacag attgtagggg ctgtagctac aaaacctgca aatactttgg
      841 caaacaggca aaatgttttt atggcggcgt tgttgtagag gaggtctatc ctgctattca
      901 ggatgcatcc accgttattc tactctgccc caactataat gatatggtga cagccaatat
      961 tgtagccaca atcaacaggc tgaccgctct tttcagaaag acaaaattct atgacaagaa
     1021 aatcttcacg gtaatcgttt caggatactc aggtggagat gcattggcca agcagctaat
     1081 tagcagcttg aatatgaaca agacctttga actgcctcca aacttttcaa ttatggccac
     1141 agccaatgat cctggggcaa tccacaaggt acctggcata agaacaaagg cagagtcatt
     1201 tgcaaaccac atacttgaaa atctatagcc tgtttacagg ctattttttt gcaaaaaaat
     1261 aaaggctcct ttccaacgat gggaggtctg accgtttcca gtcaggccct ggctacagct
     1321 ccatagactc tggtccttag aaaatgtagc tcggaaacct tttactatac atctattttt
     1381 tttcttcctt caagtccaca ttcaaatcct ctgttacctt gtttgcatgt cccttgaatt
     1441 caccaatggc cttgccaaga gattttccga tttcaggaag ctttgatggt ccgaagatca
     1501 ccaatgcaat tgcaagtatc agtataagct ctggagctcc tagtctacca aatcccatta
     1561 taacccctcc tttcgcaaat catgggctct gcttcattca ttcaaatagt tacatcataa
     1621 ttctatcttt atgcgtgaaa actgtggcat ttcctgaagc agctctccca accaaggtaa
     1681 tgcctagttt aaatccaaga tcaacagcca gtgatgtcgg attactcctg gagcagatta
     1741 ttggtacacc tgctcgggca gccttcaaca ccatatctgt tgaaattctt cctgttgtga
     1801 ttattgtaga cttactcaga tcccagcctc tatatgctgc gtatccgact accttgtcat
     1861 gagtgttgtg cctggccaca tcctccattg ttacaatatc cttcttgtac aagatggcac
     1921 tggcatgaat tccaccgtgt ctcttatact tttcagcatc ctggtacatc ctttcaaagg
     1981 ctgtctttat cctcctgaaa ttaaggagaa catcacattc acctatggcc aactgggata
     2041 tctcttccgg aaagtctaaa gtagatccgc agccagtacc atatatatca cctaaagtgt
     2101 atctcctatc tgttacattt tcagccatca catatatctg acggaggtcc tcgcaggctg
     2161 caacagtcat tatgtcagag aaggaattta ccactccagt atttataaga tgtccaattg
     2221 ccagttctct caaatctgtt ggagtgcaca ttaaggtgac cagcttttca ccattcagat
     2281 ttatggtaac aggaaactct tcagatagct ctattgcatt tatcatatcc agcatcttaa
     2341 aacctcccca ttaaggattc tcatcctttc ctacctcaat ccattctgcc agatagtcgg
     2401 catatccttt cgacatatgc ttgttggaat atacaaataa ggttccattg cttgcattgg
     2461 tgaacttaag gtctgcatat tcactatcat tggataactc tgaaagcatg tcagtcagct
     2521 caccctcagt gagattatag ggtggtagat tgaacatggt aacatatgtt ggtcttggat
     2581 atctccttga ctcctctcta acagtatcac ctatgagctt gaggatatcc ttatccttaa
     2641 ctctaattat tattcctgca tacttatctg tcatctcatt tcccgagtaa tagaaggtat
     2701 ttccctcagc atcatcgatt tttttcaagt ccaaatctgt tccaatactt tcaagtattt
     2761 gggatatatc agttgcctgg tcttcatccc catacacaag tgaggcctca atctcagcaa
     2821 gggtaactat tttcccatcc ctcgagttcc tgactataaa atctgctacg tcttcctgca
     2881 tatacttcat tttttcatcc atgtgatcac ccactacggc aacaaattca ttgattcaag
     2941 ctcactagct gcaaagtcca gaccaagtct tttcagagtt tccttggttg gagctcctgt
     3001 ttttttgtcc catcccattt cctcatagaa catggtaaga gccgtttgca tatcttctcg
     3061 ctccatcttg actgtaccct cagtgaatgg ttccatatcc ggatccatat cataaaccca
     3121 gtccattata acatcgtgct catttctcat atctacagtg cccatttgct tgactgtcaa
     3181 tgccctgtgc agggtaaata ccctttcagc tgccaaatca agtgattcct cagtgtaatt
     3241 ctctcctgta actatagaca tatatttgga ttccaaagcc gtatctccac gataatttct
     3301 ttcctttaca ggggaaacca tcattggcca catccaatta cacactgtaa gggaatcgtg
     3361 aagaacgttc cttattattg accacttggc aaattttgcc ttatattcat tcataggggt
     3421 gtactttgcc ggaaaatcga atgcttcctc agatccccat atctcagcag aaacctctct
     3481 caaaatatca gcaggtaaac ctgaatctat aagattcata tgtgtatgag attgggaatc
     3541 cctattgaac atacaggaaa tcaatgctcc tacctgggct cttgcctcat tgctgtgatg
     3601 aacgggataa cctagcggtg accaaatttt atagtcctta ccatgccaaa aatcatctcc
     3661 gaaattccat ctttttgcta tccagtaggt tccatccccc agatgactga attccccttc
     3721 cttaaatgtt atccttctat aaaagtcctt aaggaat
//
LOCUS       DASRQF010000137         3290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1242382, whole genome shotgun sequence.
ACCESSION   DASRQF010000137 DASRQF010000000
VERSION     DASRQF010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242382"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VFD79_03645"
     CDS             <1..253
                     /locus_tag="VFD79_03645"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZH93172.1"
                     /translation="RFAICKRSVMVGYSKEYLRKSQGQGFELAFPCHSPEDVDLSYVR
                     IVELGAKPVHGPKDMPWNQRTALFADPDGNIHELFAELG"
     gene            348..1184
                     /locus_tag="VFD79_03650"
     CDS             348..1184
                     /locus_tag="VFD79_03650"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZH93173.1"
                     /translation="MYRESAIHYPQDEMTTMLIPVTVGCPWNRCIFCTMYKDDKYSLV
                     PMEQIKQQLVNGYRYTEKVFLTGADPLYIGFEKMMEILEFTREYLPYCARVASYASIR
                     SVSIYSDEELSKLHHAGLRLLYIGFESGSDRFLKLMNKGHTREDAIREGKRLEKAKLA
                     YNAIILTGIGGEGTWEENARNTASMINQIHPKILITMNLILMEGSDLSALVEKGEYTP
                     APRIENLLETRNLLENLDPEKEMDFDTTHPTNIIKMKGRLPGERERLLGEIARVLNKN
                     KD"
     gene            1247..1813
                     /locus_tag="VFD79_03655"
     CDS             1247..1813
                     /locus_tag="VFD79_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HZH93174.1"
                     /translation="MIYRNATKEDIPQISELQKKYHVSTISEEDKPDGFVTTLFTPEQ
                     FGDLIDKEQGIAIACDGDQVVAYAMAASWEYWSEWPLFQHMIKDLPDTEYLGETLSTE
                     NSYQYGPICIEKSYRGTEVLPRLFDFSRAQMNKRFPILITFINHINPRSYKAHVDKLG
                     LDVIKDFQFNNNNYYELGYDTSKPVKGL"
     gene            1824..2918
                     /locus_tag="VFD79_03660"
     CDS             1824..2918
                     /locus_tag="VFD79_03660"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZH93175.1"
                     /translation="MKFYCSKCNREESVDTRKAKCECGGLWKLDYQPPKFDLEKIKSD
                     KWGLLRYREWIPVDSKALGEVSLGEGMTPAIHLDEDVMLKMDYFMPTLSFKDRGAAVL
                     VAHAKSIGVQSLVQDSSGNAGNSVAAYSAKAGIDSEIFVPEGTSPKKIAMIRSHGAKV
                     NVVPGSRDHCADVCRSKVDAEGVYYANHVFNPLFYQGTKTYIYEIYEQLRRIPENIFI
                     PLGNGTLFLGVMIGLEELLQSGIIEKMPRVFAVQGENCAPFAEAVQAGKKEIEHITPK
                     KTMAEGIAIGAPMRGDEILEYIYKYQIRVITAPEDGILPAREKLAKKGVYVEHTTAAT
                     YAAYLKLVNQEGRLEDALLPMCGAGLKSDH"
     gene            2955..>3290
                     /locus_tag="VFD79_03665"
     CDS             2955..>3290
                     /locus_tag="VFD79_03665"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HZH93176.1"
                     /translation="MDKWIEEAKKDPTANQVGMYLFHNGVVRETPKAKVRQGIDDGSQ
                     VRGMEFGYDEEKVQMAVEETRKLDGIYHVRVWLNEGELKVGDDIMYVLVGGDIRPHVI
                     DALQFLVERI"
BASE COUNT         1032 a          619 c          864 g          775 t
ORIGIN      
        1 gaggtttgcc atctgcaaga gaagcgttat ggtaggttac agcaaggaat atctgagaaa
       61 atcccaagga cagggatttg aacttgcctt tccctgccat agtccggagg atgtggacct
      121 atcctatgtg aggattgtag agctgggagc aaaaccagtg cacggaccta aggatatgcc
      181 ctggaaccag aggacggccc tctttgcgga tcctgatggg aatatccatg agctgtttgc
      241 cgagttagga tagaaaacaa cattaaccat cccgattatt tgataatcac catacccttt
      301 tcgaccctgt cgaaaggggt atttatttgg tgaaggggga tgtttttatg tatagggaaa
      361 gtgcaatcca ctatccacag gatgaaatga ccacaatgct gattccggtg actgtaggtt
      421 gtccatggaa caggtgcatt ttctgcacca tgtacaagga tgacaagtac agcctggttc
      481 ctatggaaca gataaaacag caactggtaa acggctacag gtatacagag aaggtttttc
      541 tcacaggtgc tgatccactc tatatcgggt ttgagaagat gatggaaatc ctggagttta
      601 caagggaata ccttccatat tgcgcccgtg tggcatccta tgcttccatt agaagcgttt
      661 ccatctactc tgatgaggaa ttgtccaagc tgcatcatgc aggccttaga ttgctttaca
      721 taggctttga gagtggaagt gacagatttc ttaagcttat gaacaagggg cacacaagag
      781 aggatgcaat cagggaaggc aagagacttg agaaggcaaa gctagcctac aatgcaatca
      841 tacttacagg aattggagga gaaggaacct gggaggaaaa tgcccggaac acagcctcaa
      901 tgataaacca gattcaccct aaaatcctta tcacaatgaa tcttattctg atggaggggt
      961 cagacctatc agcattggtt gaaaagggtg agtatactcc agcaccaaga attgaaaacc
     1021 ttctggagac caggaatctt cttgagaatc tagatccgga aaaggaaatg gactttgata
     1081 caacccatcc cacaaatata ataaagatga agggtaggct gcctggagaa agggaaaggc
     1141 ttcttggaga aattgcaagg gtattgaata aaaacaaaga ttaaaccata ccctgatatg
     1201 gtaaaatcat attatggatg taaagacaaa aatagaggag gccgaaatga tatacagaaa
     1261 cgctactaaa gaggacatac cacagatatc ggagcttcag aagaaatacc atgtatcgac
     1321 cataagtgaa gaggacaagc cagatggttt tgtaacaacc ttatttaccc ctgagcagtt
     1381 tggagatcta atcgacaagg agcagggaat agccatagcc tgtgacggcg accaggtcgt
     1441 tgcatatgca atggctgcat cctgggagta ttggtcagaa tggcctcttt ttcagcatat
     1501 gattaaggat ctgcctgata ctgagtatct gggggagaca ttgtccactg aaaactcata
     1561 ccagtacgga cccatatgca tagagaaaag ctacaggggg acagaggttc tgcctaggct
     1621 cttcgacttt tcaagagctc agatgaacaa gaggtttcca attctaataa cttttattaa
     1681 ccacattaat cctagatcct acaaggccca tgtggacaag ctggggctgg atgtaatcaa
     1741 ggacttccag ttcaacaaca acaattacta tgaactgggc tatgatactt caaagccagt
     1801 caagggtcta taggagggaa atgatgaaat tttactgttc caagtgcaat agggaggaaa
     1861 gtgtagatac aaggaaggca aaatgtgaat gtggaggtct gtggaagctg gattaccagc
     1921 ctccaaaatt cgaccttgaa aagatcaaga gcgacaagtg gggacttttg aggtacaggg
     1981 agtggattcc tgttgatagc aaggctctag gtgaagtctc ccttggagag gggatgactc
     2041 ctgcgattca ccttgatgag gatgtcatgc taaagatgga ttactttatg cccaccctat
     2101 ccttcaagga taggggcgcg gctgttctcg ttgcccatgc aaaatcaatc ggagtccagt
     2161 cccttgtcca ggatagtagc ggtaatgcag gaaacagcgt tgccgcctac agtgcaaagg
     2221 ctggaattga tagcgagata ttcgtaccag aggggacatc acccaagaaa atagccatga
     2281 taaggtctca tggagccaag gtaaatgttg tgcctggatc aagggaccac tgtgctgatg
     2341 tttgcagaag caaggtggac gctgaggggg tctactacgc caaccatgta ttcaatccct
     2401 tattttatca gggtaccaag acatatatct acgagattta cgagcagctg aggaggattc
     2461 ctgaaaatat ttttataccc cttggaaatg gaacattatt tttgggggtc atgattggtc
     2521 ttgaagagct gctacaatct ggaatcattg agaagatgcc aagggtcttt gccgtacagg
     2581 gtgagaattg tgcacccttt gcagaggcag tacaggcagg gaaaaaagag atagaacaca
     2641 tcactccaaa aaagaccatg gcagagggaa tagccatagg tgctcccatg cgtggggatg
     2701 agatactgga atatatatac aaataccaaa taagggtgat aacagcccct gaggacggaa
     2761 tattgcctgc tcgtgaaaaa ctggcgaaaa agggggtcta tgttgaacac acgacagctg
     2821 cgacctacgc tgcatatctt aagctggtaa atcaggaggg aagacttgag gatgccctgc
     2881 ttcccatgtg tggagccggg ttgaaatcag accattagag aaaggatgat aaaatgtcaa
     2941 aaaaatcacc atcgatggat aaatggatag aggaagccaa gaaggatcca acggcaaacc
     3001 aggttgggat gtatcttttc cacaatggag ttgtaaggga gacaccaaag gcaaaggtaa
     3061 gacagggaat cgacgatggt tcccaggtga ggggaatgga gtttggatat gatgaagaga
     3121 aggtacagat ggcagttgag gaaaccagga aactggatgg aatatatcat gtgagagtat
     3181 ggctgaatga aggggagctt aaggttgggg atgacattat gtatgttctg gttgggggag
     3241 acataaggcc tcacgtaatc gatgcccttc agttccttgt tgaaaggata
//
LOCUS       DASRQF010000138         2239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1288107, whole genome shotgun sequence.
ACCESSION   DASRQF010000138 DASRQF010000000
VERSION     DASRQF010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288107"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VFD79_03670"
     CDS             <1..361
                     /locus_tag="VFD79_03670"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZH93177.1"
                     /translation="IRQQEEMGFSTYAVELKENGELIGVCGFNPPGEGSDAEIMYHFN
                     KSYWGKGYANEAVEKTLEYARESLDISKVGALVHEENKASAAILKNNGFQFCGMKWCD
                     ATKRMEEHYQLVLKGNQ"
     gene            381..1088
                     /locus_tag="VFD79_03675"
     CDS             381..1088
                     /locus_tag="VFD79_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004839173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HZH93178.1"
                     /translation="MKKFIVEKDFWEVFPDAKIGIIIMKGIDNGIKDEGRYLDMLKDA
                     EKEALRHLPDENFSNNQVVKVWREAFQKFKTKKGARSSIEALLKRAHNGKGIGGINPL
                     VDLYNSVSLRYALPCGGEDIDTFAGDIRLTKAIGDEDFITLGSEKSEPPYEGEIVYKD
                     DEGAICRCWNWRESVRTMLREETRNAFICIESVDPSRDEDLKAALDELASLSKENLGG
                     EYEIHIVDRDRDSIEIK"
     gene            1225..1971
                     /locus_tag="VFD79_03680"
     CDS             1225..1971
                     /locus_tag="VFD79_03680"
                     /inference="COORDINATES: protein motif:HMM:NF021386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HZH93179.1"
                     /translation="MELEKFSVEELKKGYRFDQEAGSYICNTCGQNYEPGEIYKFGDR
                     LFEASKAMEMHIETDHEGSLKNMLFGETKYNTLTDNQRDLVWKMYMGMSDKEISQELG
                     ISPSTVRNQRFTLREKAKQAKLYLAIYEQAIERKTHPEDRIIPVHSSATMVDDRYVIT
                     EKERDKILNAEFESLDPLRLKRFPGKEKKKIVILGRIAQEFEPGKKYSELQMNEIIKE
                     IFDDYAVIRRYLIEYGFMDRTRDGKEYWLK"
     gene            1991..>2239
                     /locus_tag="VFD79_03685"
     CDS             1991..>2239
                     /locus_tag="VFD79_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HZH93180.1"
                     /translation="MDRRRDLKREYKESKPDMGVFMIRSKSTGKTYIEATRNLKSGVN
                     RARFTLNFGSHLHGELQCDWNEKGEADFEMEILEILEYK"
BASE COUNT          784 a          338 c          598 g          519 t
ORIGIN      
        1 tattaggcag caggaggaaa tgggattctc aacatacgca gtagagctta aagaaaatgg
       61 tgagcttatt ggagtatgcg gattcaaccc tccaggagag ggatctgatg ctgagattat
      121 gtatcatttc aacaagtcct attggggtaa gggatatgca aatgaggctg ttgaaaaaac
      181 tcttgaatat gccagggaaa gtttggatat aagtaaagtg ggcgccttgg tccatgagga
      241 aaacaaggca tctgccgcga tactaaaaaa taacggattc cagttctgtg gaatgaaatg
      301 gtgtgatgct acaaagcgga tggaagagca ctatcaattg gtgttgaaag gtaaccagta
      361 gaaaacaggg agaggatagg atgaaaaaat tcatagttga gaaggatttc tgggaagtgt
      421 ttcccgatgc taagattgga ataattatca tgaagggaat agataacggc ataaaggatg
      481 aaggcaggta tctggatatg ctaaaggatg cggagaagga agcgctgagg cacctgccgg
      541 atgaaaattt cagcaacaac caggttgtaa aggtatggag agaggcattt caaaaattca
      601 agaccaaaaa gggagccaga tcctccattg aagcactcct taagagagcc cacaatggca
      661 aggggattgg agggataaat ccattggtgg atctgtacaa ttcagtatcc cttaggtatg
      721 cccttccctg tgggggagag gacatagata catttgcagg agatatcagg cttaccaagg
      781 ctattgggga tgaggacttc ataacactag gctccgaaaa aagcgaacca ccctatgagg
      841 gggagattgt ctacaaggat gatgagggtg caatctgcag gtgctggaac tggagggaat
      901 cagtaaggac catgctaagg gaggagacca ggaatgcgtt catatgcata gaatcagtcg
      961 acccatcacg ggatgaagac cttaaggctg ccctcgatga gctggcgtcc ctgtcaaagg
     1021 agaatctggg tggagagtat gagatccata ttgtggacag ggatagggat tcaatagaaa
     1081 ttaaatagat agatcatgag catccaggat agacctgggt gctttatgtt tgccgaaaca
     1141 ataaacatta aaaacaataa ttgacattat tgataaacaa taatataata tgtttatcaa
     1201 tgaagtaaac ggaggagggt taatatggaa ctggagaagt ttagtgtgga ggaattgaag
     1261 aagggatata gatttgacca ggaagcggga agctacatct gcaatacctg cggacagaat
     1321 tatgaaccag gagaaatata caaatttggt gacaggttgt tcgaagcatc taaggccatg
     1381 gagatgcata ttgagactga ccacgaaggt agtcttaaaa acatgctttt cggtgaaaca
     1441 aagtacaata cccttactga taatcagagg gacctggttt ggaaaatgta tatgggtatg
     1501 tcggacaagg aaatttcaca ggagcttgga atttctccct caacagtaag aaatcagagg
     1561 tttacattaa gggaaaaagc taagcaggcc aaactatacc tggccatata tgaacaagcg
     1621 attgaaagga agacacatcc tgaggacagg ataataccag tgcacagttc agccacaatg
     1681 gtggatgaca ggtatgtcat aacggaaaag gaaagggaca aaatcttaaa tgcagagttt
     1741 gaaagtcttg atccattaag gctaaaaaga tttccaggta aggaaaaaaa gaaaattgtg
     1801 atccttggaa ggattgccca ggaatttgag cctggaaaga agtacagtga actccagatg
     1861 aatgagataa tcaaggaaat ttttgatgac tatgcagtca tcagaagata cttgatagaa
     1921 tatggattta tggacagaac aagggacggc aaggagtatt ggttaaaata atgaaaaaga
     1981 ggttatgaaa atggatagaa gaagagatct taaaagagaa tacaaggaat caaagcccga
     2041 tatgggtgtt ttcatgatca ggtccaagtc tactggtaag acttatattg aggctaccag
     2101 gaacttgaaa agcggcgtga acagggctag atttaccctt aactttggga gtcacctcca
     2161 tggtgaactt cagtgtgatt ggaatgaaaa aggcgaagct gactttgaaa tggagattct
     2221 tgagattctt gaatacaag
//
LOCUS       DASRQF010000139         2906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_370870, whole genome shotgun sequence.
ACCESSION   DASRQF010000139 DASRQF010000000
VERSION     DASRQF010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370870"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VFD79_03690"
     CDS             complement(<1..615)
                     /locus_tag="VFD79_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93181.1"
                     /translation="MILNHDAFQIGEDYGGKQQWLSEFGLRKKFWADRSCGVAAAANL
                     INYLGSEFPELATLYGGNTREDFIQLMENLYGYLKPGVFGIPTLGKMGRGLTGYTNSK
                     GIQLLPCTRTWIFDVKDGSKFIRGGLAQGTPVLLLTWNYSDRELRNHWVTVTGWDVVY
                     GEEYMTVSNWGKKRTYSYDKWIGERSLLKGAIYLTRNKCIDAPDM"
     gene            complement(630..1529)
                     /locus_tag="VFD79_03695"
     CDS             complement(630..1529)
                     /locus_tag="VFD79_03695"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZH93182.1"
                     /translation="MFDQRTKTFLEVCKWMSYTKAAEALFITQPAVTQHIKFLETRYN
                     TRFFSYANRQIGLTPAGREFKRYLKEGMAKERLVSEKIRELDKGERTIKFGATLTIGE
                     YTIAPFINDFHDKYKDYRISIQIDNTRILMEELDSGGIHFALIEGLFNKSDYDSRLLA
                     MKDFILVVHPASGLANKNRPVLEDILKETLIIREKGSGSREVLESGLAERNHSLDDFQ
                     ETISIGNVSLMKRMVQDGLGITFMYEDAAKVELDTGKLAKVDLGDFSISREFNFVSIT
                     DSPFKDELDSLYGYFKENINSFK"
     gene            1634..2650
                     /locus_tag="VFD79_03700"
     CDS             1634..2650
                     /locus_tag="VFD79_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015778069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HZH93183.1"
                     /translation="MNTISKSVAPGLLLSVLLAIVSVHISNSLPSHIIGASVLALILG
                     MLINPFITSYPIFAKGLTFTSKKVLKFAIILMGVRLSFQQIFQVGRFSLVVMTFTLLA
                     AFGGGYLLGRVFKMDWRLSSLISAGTGICGGSAVAAVAPVIDSDESQIAYAISATFIF
                     DVIMVVLFPLMGVYFGLSDLGFGLWTGTAVNDTSSVVAAGYAFSDAAGDYAIIVKLTR
                     TLAIVPVVLVFSWIGARVKRNECKETEICNVSIKDIFPWFILLFIAMVGIASTGIFQE
                     GTRALMSDTSRFLMVMALGSIGLKTNFSNLAKSGFLPMVHGFLISTLVVVVSFAVQSL
                     LGQI"
BASE COUNT          753 a          658 c          584 g          911 t
ORIGIN      
        1 catatcaggg gcatcaatac atttatttct ggtaagatat atggctccct taaggaggga
       61 cctttcccca atccatttgt cgtaggaata ggtcctcttt ttaccccagt ttgaaacagt
      121 catgtattcc tcaccatata ccacatccca accggttacg gtaacccagt gattcctaag
      181 ctccctgtcc gaatagttcc aggttagcaa tagaactggg gtaccttgtg caagtccgcc
      241 cctaatgaac ttactgccat ccttaacatc aaaaatccag gttcttgtac agggtagtag
      301 ctggattccc ttgctgttgg tgtagcctgt caaacctctc cccatcttac ccagggttgg
      361 aattccaaaa actccaggct tgagataacc atataggttt tccataagct ggatgaagtc
      421 ctcccttgta tttcctccat aaagggtcgc cagctcagga aactcacttc caaggtaatt
      481 gatcagattt gcagccgcag caaccccaca ggaccggtct gcccagaact ttttcctaag
      541 gccaaattct gaaagccact gctgtttacc gccatagtcc tcccctattt ggaaggcatc
      601 gtggtttaga atcatgaata atcttccttt cacttgaaac tgtttatatt ctccttaaag
      661 tatccatata ggctgtcaag ttcatcctta aaaggcgaat cagtgatgct tacgaagtta
      721 aactcccttg aaatcgaaaa atcacctaaa tccactttag ccaacttacc tgtgtccagc
      781 tccacctttg ctgcatcctc atacataaag gtgattccaa gcccatcctg gaccatcctt
      841 ttcatcaggc tgacattccc aatgcttatt gtctcctgaa aatcatccag ggagtgattc
      901 ctttcggcca gaccgctctc caagacttct ctggacccag aacccttctc cctaattatc
      961 aatgtctcct ttaatatgtc ctcaagaacc ggtctgttct tattggctag accgcttgca
     1021 ggatggacga ccagaatgaa atccttcatg gccagcagtc ttgagtcata gtccgacttg
     1081 ttgaatagac cttcaatcag ggcaaaatga atccctccac tatcgagttc ctccataagt
     1141 attctagtgt tgtcaatctg gattgagatc ctgtaatcct tgtatttgtc gtgaaagtcg
     1201 tttatgaagg gagcgattgt atattcaccg attgtcaatg tagccccgaa tttaatggtc
     1261 ctttctccct tgtcaagctc ccttatcttt tctgatacca gcctttcctt tgccatccct
     1321 tctttaagat atcttttaaa ctcccttcct gcaggggtta atccaatctg cctgttggca
     1381 taggaaaaaa accttgtgtt atacctggtt tcaaggaact taatgtgctg tgtcacagca
     1441 ggctgtgtaa tgaagagggc ctccgctgcc tttgtgtagc tcatccattt acaaacctca
     1501 agaaatgttt tggttctctg gtcaaacatt taatcacttc cataaattat atttatctat
     1561 ttataattat atataatttt atattatatt aaaagagtga tagaatcaaa ttatatttga
     1621 aaggaggcct agtatgaata ccatatcaaa atccgttgca ccaggacttc ttctgtcagt
     1681 cctccttgca attgtatctg tacatatcag caattcattg ccatcacata ttataggggc
     1741 aagcgtactg gcgctgatcc taggtatgct aatcaacccc tttatcacaa gttacccaat
     1801 attcgccaag ggattgacat tcacatcgaa aaaagtcctt aaatttgcaa taatccttat
     1861 gggggtcaga ctaagcttcc agcagatatt tcaggttggc aggttttccc ttgtggtaat
     1921 gacctttaca ctacttgctg cttttggtgg tggatacctc ttgggaaggg tgttcaagat
     1981 ggactggaga ctctccagcc ttatttctgc aggaacgggc atttgcggcg gttctgccgt
     2041 tgctgccgtt gctcctgtaa ttgattctga cgaaagccag atcgcatatg caatttcagc
     2101 aacctttatc tttgatgtaa taatggttgt tcttttccct ttgatgggtg tctattttgg
     2161 cttaagtgac ctgggatttg gtctgtggac aggaactgca gtaaatgata cctcatcggt
     2221 tgtggcagca ggctatgcat tttcagatgc agccggagat tatgcaataa ttgtcaaact
     2281 tacaagaact cttgccattg taccggttgt acttgtcttt tcctggattg gtgccagggt
     2341 caaaagaaat gaatgcaagg aaactgaaat ctgcaatgta agcatcaagg atattttccc
     2401 ttggtttata ctccttttca ttgctatggt tggaattgca agtacaggca tctttcagga
     2461 gggaaccaga gctttgatgt cagacacaag caggtttcta atggttatgg ccctgggatc
     2521 cataggtttg aagaccaact tttccaatct ggcaaagtca ggatttctcc ccatggtcca
     2581 tggatttttg atttcgacct tggttgtggt ggtctccttc gctgttcaaa gtctacttgg
     2641 tcaaatctag ttcaagagaa ctctcaaaac tccggtcttc cggagttttt tcattataat
     2701 ttgggtataa tgatatgagt aacagtgaaa ggtggtgatt ttttgtcatt aaataagaag
     2761 gttgcagtag taggcggaat aaatatggat attgcaggat ttcctcacgg acaactcatt
     2821 cccggggatt ccaatccagg aagaactaag ctttcatctg gcggtgtcgg aaggaatatt
     2881 gctgaaaatg tagcgctttt ggggaa
//
LOCUS       DASRQF010000140         2360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_10814, whole genome shotgun sequence.
ACCESSION   DASRQF010000140 DASRQF010000000
VERSION     DASRQF010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10814"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /locus_tag="VFD79_03705"
     CDS             complement(<1..1219)
                     /locus_tag="VFD79_03705"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HZH93184.1"
                     /translation="MSCNEWKEVSVFEIGHVITGKTPSSKIPNQFGKLLPFVTPTDFN
                     NYHKWVYEAERYLSDDGVSRLQGKVLPTNSVLVTCIGSDMGKVAISGVKCVTNQQLNS
                     IIPFPKKVDSNFLYYTFGSMRGYLQQLARGGTTMPIINKGTFEKIKLILPSLVEQKAI
                     AHTLSCLDDKIELNNRINKTLEEMAQAIFKSWFVDFEPFQDGEFEDSELGPIPKGWKV
                     RTIGEIAEVIDCLHSKKPERCQEGYPYLQLNNIRNDGLLDLSDIYLISNSDYQKWISR
                     VEVSQGDCVITNVGRIGAVAQIPPVFKAAIGRNMTSIRCKTIFPYPTFLIECLLSKQM
                     KNEINLRTDSGTILNALNVKSIPKLRFVHPSDETLQKFEDTVRPIRGAMEQNLEENRC
                     LTYIRDTLLSKLMS"
     gene            complement(1209..2327)
                     /locus_tag="VFD79_03710"
     CDS             complement(1209..2327)
                     /locus_tag="VFD79_03710"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HZH93185.1"
                     /translation="MDKTKLGELIDLFSFNLGNKEAKAQDVLGRVYEYFLGKFGSSQG
                     EFYTPPSIVKLLVGMIEPYEGRVYDPCCGSGGMFVQSKKFVEEHQGRKDDIHVYGQEY
                     TASTWRLCKMNLAFRGIDGNLGKRDADTFGNDLHKNLRADFVLANPPFNMSDYTLIQD
                     DARWKYGIPPKNNANYAWIEHMVSKLSPKGIAGFVMANGSMSTSTKAEAEIRKNMIEA
                     GLVDCIVTMPPNLFYNVTIPVCLWFISKNKENRKDKILFINARKMGTMVTRKFRDLSD
                     EEIQRICDTYHNWRDGKEEYEDIQGFCKSADLEEVRSHDYILTPGRYVGIEEVEEDGE
                     PFEEKMERLTGELAELFAKSHKLEEEIKQRLGAIGFEL"
BASE COUNT          586 a          567 c          497 g          710 t
ORIGIN      
        1 ctgacattaa tttggagagt agtgtgtcac gaatataagt caggcaccga ttttcctcaa
       61 gattttgttc catggctcct ctaataggtc ttactgtatc ttcaaacttc tgaagagttt
      121 catcacttgg gtgaacaaat cgtaatttag gaatgctctt cacatttaat gcgttgagta
      181 ttgtcccgct atcggtcctt agatttattt cattcttcat ctgctttgac aacaagcatt
      241 caataagaaa agtcgggtat gggaatatag tcttacagcg gatgctagtc atatttcttc
      301 cgattgccgc tttaaaaaca ggaggtatct gtgcaactgc cccaatccta cctacgttag
      361 taatgacaca gtcgccttgg gagacctcaa ctctagaaat ccacttctga taatcagaat
      421 tacttattaa gtagatatct gataaatcaa gtaacccatc attacgaatg ttgtttagtt
      481 gaagataggg atatccttcc tggcacctct caggtttttt tgagtgcaga caatcaatta
      541 cttcggcgat ttctccaatc gttctcacct tccaaccctt gggaatcggg ccgagttcgc
      601 tgtcttcaaa ttcgccgtct tggaagggct caaagtccac aaaccagctt ttgaagatgg
      661 cttgggccat ttcctcaagg gtcttgttga tgcgattgtt gagttcaatt ttgtcatcga
      721 ggcaggaaag ggtatgggct atagcttttt gttctactaa tgaaggcaat attaatttta
      781 tcttctcgaa agtacctttg ttgataatag gcattgttgt gccaccacgc gctagttgtt
      841 gcaggtagcc acgcatacta ccaaaagtat aatacagaaa attggagtca acttttttgg
      901 ggaatggaat tatagaatta agctgttgat ttgtcacaca ttttacaccc gaaatagcga
      961 cttttcccat gtcagaccca atacatgtca ctaaaacaga attcgttggc agtacctttc
     1021 cttgtagcct ggaaacccca tcatctgata gatatctctc cgcttcataa acccatttgt
     1081 ggtaattgtt aaaatctgtt ggtgtaacaa agggtaacaa ctttccaaac tggtttggta
     1141 ttttactgga aggagtcttt ccagttatta cgtgtccaat ttcaaagacg cttacttctt
     1201 tccattcgtt acagctcaaa cccaatcgcc cccagtcgct gttttatctc ttcctccagc
     1261 ttatgagact tggcaaagag ttcggccagc tcccctgtca gcctctccat tttctcctca
     1321 aagggttcgc cgtcctcctc gacctcttca atacccacat acctgcccgg ggtcaggatg
     1381 taatcatggc tccttacttc ctccaggtca gcggacttac agaaaccctg gatatcctca
     1441 tactcttctt tgccgtcccg ccaattatgg taggtatcgc aaatacgctg gatttcctca
     1501 tcagagagat ccctgaattt gcgggtgacc atggttccca tcttacgggc attaataaat
     1561 agaatcttgt ccttcctgtt ctccttgttc ttagagataa accacaggca cacgggtatg
     1621 gtcacattgt agaaaaggtt tggcggcatg gtgacgatgc aatcaaccag acccgcctca
     1681 atcatattct tcctgatttc tgcttctgcc ttggtggagg tgctcataga gccgttggcc
     1741 attacgaagc cggcaattcc cttaggagac agcttgctaa ccatatgctc aatccaggca
     1801 tagttggcgt tgttcttggg tggaatccca tacttccagc gagcatcatc ctgaatcaat
     1861 gtataatcgc tcatgttgaa ggggggattg gccaggacga agtctgcccg gaggttttta
     1921 tgcaggtcat ttccgaaggt atctgcatcc ctcttcccca ggttgccgtc aataccgcgg
     1981 aaggccaggt tcatcttaca taggcgccag gttgaagctg tatactcttg accataaaca
     2041 tggatgtcat cttttctgcc ctgatgttcc tcaacaaact tcttggattg gacaaacata
     2101 cctccagatc cgcagcaggg gtcatatacc ctaccttcgt agggctcaat cattccgacc
     2161 aggagtttga caatactggg gggagtgtag aactcgcctt ggctggaccc gaactttccc
     2221 aggaaatatt cataaacccg gcccaggaca tcctgggctt tggcttcttt atttcccagg
     2281 ttgaaggaga acaggtcaat aagttcaccc agcttggtct tgtccaactc ggggcgggcg
     2341 taatttttgg ggaggacccc
//
LOCUS       DASRQF010000141         3546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_432460, whole genome shotgun sequence.
ACCESSION   DASRQF010000141 DASRQF010000000
VERSION     DASRQF010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432460"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..1106
                     /locus_tag="VFD79_03715"
     CDS             9..1106
                     /locus_tag="VFD79_03715"
                     /inference="COORDINATES: protein motif:HMM:NF008519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZH93186.1"
                     /translation="MMYDVIIVGGGPAGASLARLLGKSRKVLVIERRDMQRISGNDYK
                     KCCGGLVAPDAQKLLAEFGLGIPVEVLDGPQLFAVRTIDHEHKLERYYQRHYININRE
                     RFDRWLLSMVPTSVHIEDRSIFKDYLQFGNRVQVTYLKDGEEKRATAKLLVGADGASS
                     RVRRQLEPDRKGPKKYIAIQEYFRQTGDQPYYGSIFDKDITDFYSWTIPKEGVLVVGS
                     ALDLDREPIEKFEELKGRLQKEGFDLGKSLGRNSAFLFRPRGTGQILTGKGKVVLIGE
                     AAGWISPSSAEGLSYGFKSALNLSLAIDENMDRAAVIYRRNSKSLYLNIKWKGIKSFL
                     MYNDYTRYMIMRSGILSMGSIKERLAETGII"
     gene            1162..1965
                     /locus_tag="VFD79_03720"
     CDS             1162..1965
                     /locus_tag="VFD79_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZH93187.1"
                     /translation="MKRVVTFYFSPTGTTEKVVMEIASALADKEGCGMVHHDFTLPAG
                     REAYPDFREGDIIIAGVPVYAGRVPNVLLKYLSGLKGNGAKAVAVVLYGNRAYDDALL
                     ELRDILKHGGFNVMGAGAFIGEHSFSYDLAKGRPNEGDLELAREFASRLDFDSGYGPE
                     DLDIYGNPDYKEYYKPLDEEGKPVDIRRVQPKTGNACIDCKICVKVCPMGSIPHGNVK
                     ILTGICIKCGACIKKCPVEAKYFDDPDYLRHKWELEEEFKDWKLPEFFV"
     gene            2030..3097
                     /locus_tag="VFD79_03725"
     CDS             2030..3097
                     /locus_tag="VFD79_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93188.1"
                     /translation="MNIKSIKPKEIAMGFTGSLLGAGFVSGQEIMQFFGVFGTYGLIA
                     MPISLALFYILGCAVMKTARKTGITEFDKIIIRKEIPWLRALFGLTFIFFLFGTVVIM
                     TAGTGALLNQHFGVPTILGSAVMAILLGIVALWEAKGVLTAFSITVPLMIVVGMVTGV
                     FSVFQFQPEVIISSPTSGSNPLLGNILFSTTAFISYNMMAAISILVPISPDVDDESTI
                     HKGMLRGIVQLSIVFICILLPMVFFRTMLTDAELPMVALATMIYPILGTIYSFLLFIG
                     MFGSALSCLFGAAYRIRKYRDYKLRPLIGILISVAFVGSMLGFKELIGIVLPVSGYIG
                     FFAMIGITRHYLSIRKTGEVE"
     gene            3098..3484
                     /locus_tag="VFD79_03730"
     CDS             3098..3484
                     /locus_tag="VFD79_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZH93189.1"
                     /translation="MKAEINLITIWTDDVEGMKDFYTRVIGFEPINDLGDYVEFGSRG
                     VRFAICKRSVMVGYSKEYLRKSQGQRFELAFPCHSPEDVDLSYERIVKLGATPVHGPK
                     DMPWNQRTALFADPDGNIHELFAELG"
BASE COUNT         1017 a          654 c          914 g          961 t
ORIGIN      
        1 ggtgagatat gatgtacgat gttatcatcg taggtggtgg acctgcagga gcaagcttgg
       61 ccagactctt gggaaagagc aggaaggttc ttgtgattga acggagagac atgcaaagaa
      121 tcagcgggaa tgactacaag aagtgctgtg gaggccttgt ggcacctgat gcccagaaac
      181 ttttggcaga gtttggtctc gggattccag tagaggtttt ggatggtccg cagctttttg
      241 ccgtcaggac cattgaccat gagcacaagc ttgaaagata ttaccagaga cactatataa
      301 acatcaacag agaaaggttt gacagatggc tcctttctat ggttcccaca agtgtccata
      361 ttgaggatag gtcaatcttt aaggactacc tccagtttgg gaacagggtt caggttactt
      421 acttaaagga tggagaggaa aaacgggcga ctgccaagct cctggttggg gcagatggtg
      481 cctcttcaag ggtcagaaga cagctggaac ctgacaggaa agggccaaaa aaatatatag
      541 ctatacagga atactttaga cagactgggg atcagcccta ctacgggtca atatttgaca
      601 aggatatcac tgacttctat tcctggacaa tccccaagga aggggttctt gttgttggaa
      661 gtgccttgga tctagatagg gaacctatag agaaattcga agaacttaag ggaaggcttc
      721 agaaggaggg ttttgacctt ggaaagagcc ttgggagaaa cagtgcattc ctgtttagac
      781 caagaggaac agggcagatt cttacgggaa agggaaaggt tgttctaata ggtgaggcag
      841 ccggttggat tagtccaagc tcagcagaag gcttaagcta tggattcaag agcgccttga
      901 acctttctct agcaattgat gaaaatatgg acagagctgc agtaatctac agaagaaatt
      961 caaaaagtct atatttaaac attaaatgga agggcatcaa gtcatttttg atgtataatg
     1021 attatacacg gtatatgata atgaggtcag gtatactttc aatgggttct atcaaggaaa
     1081 ggctggcaga aacagggatt atctaaagca tcggggacgg ttctttttgc ttttgagagt
     1141 ggatattatg aggtggtaaa gatgaagagg gttgttacat tttatttcag cccaacaggt
     1201 acaactgaaa aggtagtgat ggaaatagct agcgcccttg cggataagga aggctgcggc
     1261 atggttcacc atgattttac actgccagct ggcagagaag cctaccccga tttcagggaa
     1321 ggggatatta ttatagctgg tgttccggta tatgctggaa gggttcccaa tgtacttctt
     1381 aaataccttt cagggctgaa gggtaatgga gccaaggctg tagcagtggt tctctacggt
     1441 aacagggctt acgatgatgc ccttctggag cttagggata ttctgaagca tggaggtttt
     1501 aatgtaatgg gagccggagc ctttataggg gagcattcct tttcatatga ccttgccaag
     1561 ggtagaccga atgagggcga tctggaattg gccagagaat ttgccagtag gcttgatttt
     1621 gattccggtt atggaccaga ggacctagat atttatggta atccagacta caaggaatac
     1681 tacaagcctt tggatgagga aggcaagcct gttgatatca ggagggttca gccaaagaca
     1741 gggaacgcct gcatagactg caagatttgt gtgaaggttt gtccaatggg atcaatcccc
     1801 catgggaatg taaaaatcct aactggaata tgtataaaat gcggagcctg catcaagaag
     1861 tgcccggttg aggctaaata ctttgatgac ccagactacc taaggcataa atgggagctt
     1921 gaggaggagt ttaaggattg gaagctgcca gaattctttg tttgagtcac tggggacggt
     1981 tatttttgac tcaaaattct acataaattc acccaaggaa gggatataat tgaacataaa
     2041 gagtataaaa cctaaggaaa tagcaatggg atttacagga agtcttttgg gagcaggctt
     2101 tgtttcaggc caggaaataa tgcagttctt tggtgttttt ggcacatatg gacttatagc
     2161 catgccaata tcccttgcac tcttttacat acttggctgc gccgttatga agacagccag
     2221 aaaaaccgga ataactgagt ttgacaagat aatcatacgg aaggaaatac catggttgag
     2281 ggccttgttt ggactaacct tcatcttttt tctttttgga acggttgtaa taatgacggc
     2341 aggtacggga gcccttctca accagcattt tggagtacct accattttag gaagtgccgt
     2401 catggccata cttcttggca tagtagcact ttgggaggca aagggagtcc ttacggcttt
     2461 ttcaataacc gtgcccctga tgattgtggt tggaatggta acgggtgttt tttcggtttt
     2521 tcagtttcag cctgaagtta taatctcctc acccacttca ggatccaacc cattgttggg
     2581 aaacattctc ttttcaacaa cagcctttat ctcctacaat atgatggcgg ccatttcaat
     2641 actagtgccc atttctccag acgttgatga cgaaagtaca atacataagg ggatgctccg
     2701 tggaatagtg cagctatcca tagtttttat atgcatatta ctgcctatgg tattcttcag
     2761 aaccatgctt acagatgcag agcttccaat ggttgccttg gcaacaatga tctatcccat
     2821 attgggaaca atatactcct ttctcctatt tataggcatg tttggcagcg cattgtcctg
     2881 tctgtttgga gcagcctata gaataagaaa gtacagggac tacaagctta gaccccttat
     2941 aggaatcctc atatcagtcg catttgtggg gagcatgctt ggctttaagg agcttattgg
     3001 gattgtattg ccggtttcag gctacattgg cttctttgca atgataggga taacaagaca
     3061 ctatttgtca atcagaaaaa ccggggaggt tgaataagtg aaagctgaaa ttaatctcat
     3121 aaccatttgg acggatgatg ttgaaggtat gaaggatttt tacaccaggg tcataggctt
     3181 tgagccaata aatgatcttg gggactacgt ggagtttgga agcaggggag tgaggtttgc
     3241 aatctgcaag agaagcgtta tggtaggtta cagcaaggaa tatctgagaa aatcccaagg
     3301 acagagattt gaacttgcct ttccctgcca tagtccggag gatgtggacc tttcctacga
     3361 gaggattgta aagctgggag caacaccagt tcatggacct aaggacatgc cctggaacca
     3421 gaggaccgcc ctatttgctg atcctgatgg gaatatccat gagctgtttg ccgagttagg
     3481 atagaaaaca acattaacca tcccgattat ttgataatca ccataccctt ttcgaccctg
     3541 tcgaaa
//
LOCUS       DASRQF010000142         3379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_373968, whole genome shotgun sequence.
ACCESSION   DASRQF010000142 DASRQF010000000
VERSION     DASRQF010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373968"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="VFD79_03735"
     CDS             <1..1191
                     /locus_tag="VFD79_03735"
                     /inference="COORDINATES: protein motif:HMM:NF022641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HZH93190.1"
                     /translation="TPENTEESERYRSLCEESGWKFITSADYLQFFYADEDSDPVPIQ
                     TDQVLEQELVEHTLLRNELRGFFLVSFILAFLIFRSLPIKYHILLSFTGVAGIFLLPT
                     VYILTTIPAAYSIFRTLKARRNIKRGLPLEMPTMKSARRRIVLFSGSIWCVSVVFLMF
                     MMVDVLYAPQLAVRVFLGPGIGIIIGASLRYYTKKKAKRVEDSIPAIMFAVVALIIFV
                     NTAVPYALGRVEDSYIVDSIPEGYPVVTIDEISKGAFRASSTTMDFRPGSSPVVPRHY
                     SYREYASVNGHNKGLNVKYYKTIDPYYADIVFTGIGRELEEGIRWQGMTIFDRTLISD
                     EEMKNLWGVEDLVLTQERDMMVIRDGGVVLRLSGDMDFRDIKTRELIMKRFFADHPRE
                     KQKM"
     gene            1339..1962
                     /locus_tag="VFD79_03740"
     CDS             1339..1962
                     /locus_tag="VFD79_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93191.1"
                     /translation="MKRITPFMYFVLATVLLLGCGKEELKSIDNNQETIEETAVGQAE
                     EFEVKEVPHDEFRVESIKREEVGEYTLIREVELENVFAENESLRLEVVKYQLNTLKPK
                     DEYREFFMEMKSGDSEGVTILDVTLEVENLSEDILEPGFLQGVLVINNGEEIPVIFGF
                     EDEGNEGPFEFLGKEVKEGSISAVISSDPEVAESFRLVYGNIELSFK"
     gene            2086..2862
                     /locus_tag="VFD79_03745"
     CDS             2086..2862
                     /locus_tag="VFD79_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZH93192.1"
                     /translation="MRERMLTIEEFTSKILNNTREINIYLPPSYSRDIHVSYPVLYMH
                     DGQNIFDGERAFAGVGWKVHEIHDELVERGMIRELIIVGIDNMQEERLSEYAHTNGRY
                     KRTRIKAKGKLYERFLIEELKPFVENNFRTLPGPDNTGLMGSSMGGLVTFNIGFRNPH
                     VFGRLGIISPSFWWGTRANIKTVDIHQDALAISRMWIDMGTKEGGLDRSFDRVVERIL
                     SKCDSSKGNIEAHWIMGAAHTELDWQRRVHWPLIHLFGLD"
     gene            2961..3308
                     /locus_tag="VFD79_03750"
     CDS             2961..3308
                     /locus_tag="VFD79_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93193.1"
                     /translation="MKREHKRTLKILVPAIAIELFFLFRRGFVISESFFFIWFVVFGT
                     FFAFRWIGGSASPMGIGGRSDRFLAAKFSETMYGGETSEKVKNSDFGLDLIFLFLAVA
                     NLVLSLVAYYIER"
BASE COUNT         1039 a          542 c          861 g          937 t
ORIGIN      
        1 actcctgaaa acactgagga atcagagcgg tatagaagtt tgtgtgaaga atccggatgg
       61 aagttcataa catcagctga ctatttacag tttttttatg cagatgaaga cagtgaccct
      121 gttcccattc aaactgacca ggtgttggag caagagcttg ttgagcatac cctgttgagg
      181 aatgagttga gaggcttttt cctggtatca tttatcctgg catttctcat tttcagaagt
      241 ttacccatca agtatcatat tctactatcc ttcacgggtg ttgcaggaat atttcttctg
      301 ccaacggtct atattttgac tacaatccct gctgcctata gcatctttag gactttgaag
      361 gccaggagaa atatcaagag aggcctgcca ctggaaatgc ccaccatgaa aagtgcacga
      421 aggcgaatag ttttgttttc cggatccatc tggtgtgttt cagtggtttt tcttatgttt
      481 atgatggttg atgtacttta cgcacctcaa cttgctgtca gggtattttt gggacctggc
      541 attgggatta tcattggagc cagtcttcgg tattacacaa agaaaaaggc gaagagggtg
      601 gaggatagca taccggcaat catgtttgca gtggtcgcgt tgatcatatt tgtgaatacg
      661 gctgtgccat atgctcttgg aagggttgag gattcctata ttgtagattc aatacctgaa
      721 ggctatcccg tggttacaat tgatgaaata tcaaaggggg cgtttagggc aagctcaacc
      781 accatggact tcaggcctgg gagcagcccc gtcgtaccaa ggcactactc ctaccgggag
      841 tatgccagtg taaatgggca caacaagggt ttgaatgtaa aatactataa aacaatcgat
      901 ccatactacg ctgatattgt atttacagga ataggaaggg aactggaaga agggatacgt
      961 tggcagggaa tgaccatatt tgatagaacc ctcatatccg atgaagagat gaagaattta
     1021 tggggtgtgg aggatttggt gttgacccag gaaagagaca tgatggtaat cagagatgga
     1081 ggagttgttc taaggctctc aggggatatg gatttcaggg acattaaaac cagggagctg
     1141 ataatgaaaa gatttttcgc agatcatcca agggaaaagc aaaaaatgta ataaccaggc
     1201 tgtctagagt ccctggcaga gtgggaaagc atccaattat ttacaaatca attacagttc
     1261 tcctttaaca gaccatcaga acatataaag tgatatgata gatgaactag acccaacaaa
     1321 aaaggaggaa acgtagttat gaaaagaata accccattta tgtattttgt cttggcaacc
     1381 gttcttctgt tgggatgtgg caaagaagag cttaagagca ttgacaacaa tcaggagact
     1441 attgaagaaa cggctgttgg gcaggcggaa gagtttgaag taaaggaagt tccccatgat
     1501 gaattcaggg tagagtccat taaaagagaa gaggttggag agtatacatt gattagagaa
     1561 gttgagcttg aaaatgtttt tgctgaaaat gagtccctaa gattggaagt tgttaagtat
     1621 caattgaaca cgctgaaacc taaggatgag tacagagagt tttttatgga gatgaagtct
     1681 ggagactcgg aaggtgtaac aatcctggat gtgacccttg aagttgaaaa cctgtcagag
     1741 gatatcctgg aaccaggttt tcttcaaggt gtattagtta taaacaatgg tgaagagata
     1801 cctgttatat ttggatttga ggatgaagga aatgaaggtc cattcgagtt tttaggaaaa
     1861 gaagtaaaag aaggtagcat cagtgcggtt ataagttcag atcctgaggt tgctgagagt
     1921 tttagattgg tttatggcaa tattgagcta agctttaagt gataaaaggg acaggttttg
     1981 tcctacagaa aaggcataac ggaatgcttg atgatgaaat cgagtaagac aaggggacag
     2041 gaacactgtc ttattttaga tgaatgggat gaaaggatga accacatgag agaaagaatg
     2101 ttaacgattg aagaatttac ttctaagatt ctcaataaca ccagggagat taacatatat
     2161 ttgccaccaa gctactccag ggatatccat gtgtcatatc ctgtacttta tatgcatgat
     2221 ggacaaaata tctttgacgg tgaaagggct tttgctggag ttggatggaa ggtacatgaa
     2281 atccacgatg agttggttga aagaggtatg atcagggagc ttataattgt tggaatagac
     2341 aatatgcagg aagaaaggct aagtgagtat gcccacacca atggcaggta caaaaggacc
     2401 agaatcaagg caaagggaaa gctttatgaa agatttctca ttgaagagct taagcctttt
     2461 gtagagaata acttcaggac acttcccggt ccggacaaca caggtctcat gggatcatca
     2521 atgggtgggc tggtaacctt caacatagga tttaggaatc ctcatgtgtt cggaaggctt
     2581 ggaataatat ccccctcctt ctggtggggt accagggcta atatcaagac tgttgatata
     2641 caccaggatg cccttgccat cagtcgcatg tggatagata tgggaacaaa ggagggaggc
     2701 ttggacaggt cctttgacag ggtggttgag cgtatcttaa gtaaatgcga cagttcaaaa
     2761 gggaatattg aggctcattg gataatggga gcggcccaca cagagcttga ttggcagaga
     2821 cgggtccact ggcccctgat tcacctgttt ggacttgatt aaaatgaaaa tattcaaaat
     2881 tgaacatttt gcaattaagt tgggtatgat aattctaaag gatatgttaa gaactaaacc
     2941 tagtccaagg gagtgaaaag atgaaaagag aacacaagag aacccttaag attttagttc
     3001 ctgcaattgc aatagagtta ttctttcttt tcagaagagg ttttgtaatc agcgagagtt
     3061 tcttctttat atggtttgtc gtatttggga catttttcgc cttccgttgg attggtggca
     3121 gtgcaagtcc aatgggcatt ggtggaaggt cggataggtt tcttgctgcc aagttcagcg
     3181 aaaccatgta cgggggagag acctccgaaa aggtcaagaa tagtgacttt ggtctggacc
     3241 tgatattcct gtttcttgca gttgcaaatc tggtattgtc ccttgtagcc tattacatag
     3301 agagataatg ttgattttga aaggtgtgat cttatggaat ttgtaaagtt tggaaacaag
     3361 tatatagtaa gattggaca
//
LOCUS       DASRQF010000143         2680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_98574, whole genome shotgun sequence.
ACCESSION   DASRQF010000143 DASRQF010000000
VERSION     DASRQF010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98574"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1160
                     /locus_tag="VFD79_03755"
     CDS             144..1160
                     /locus_tag="VFD79_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HZH93194.1"
                     /translation="MNIREQFEQRERQTLSSRACLSMNTKGRKVPEEKCQIRTEFQRD
                     RDRVIHSKAFRRLKHKTQVFIAPEGDHFRTRLTHTLEVAQLARTMARALRLNEDLVEA
                     ISLAHDLGHTPFGHTGEKVLDRVNPRGFKHNLQSARVVELLEGSEKRQGLNLTYEVLE
                     GIINHSGDNVSKTLEGQIIKFADRIAYINHDIDDAMRAGVISEKDLPADCIQVLGKTH
                     GDRINTMIVDLIENNQESDRLTMSDLIGEKTNKLRQFMFDNVYLNKEAKKEDDKAEYI
                     IEVLYNYYRKNFHRLPSEHLGIYKSVEVEKEDIICDYIAGMTDRYIVNLYMDLFVPKP
                     WGKY"
     gene            1204..>2680
                     /gene="dnaG"
                     /locus_tag="VFD79_03760"
     CDS             1204..>2680
                     /gene="dnaG"
                     /locus_tag="VFD79_03760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HZH93195.1"
                     /translation="MSYANEDVLEKVKDQMDIVQLVGEYVHLKKSGSNHIGLCPFHNE
                     KTPSFTVSESKQIFHCFGCGEGGDGISFVMKKENLDFVDALKFLADKYGIPWDENPDR
                     DDSREKSNLYNINKEAARYFYNNLLNNKKPREYLAKRGITGEIARKFGLGYATDKWDG
                     LIKHLLEKGFTEDEMVKAGLAGIRKDGSGYFDKFRNRVIFPIIDTRSRVIGFGGRVLD
                     HNLPKYLNSPDTMVFNKGYHLYGLNLLAGNTDRRRILLVEGYMDVISLYAGGVNYAVA
                     SLGTALTSNQGKLLKRYGDEVLICYDRDRAGIKATLRAIDVLRELEVDPKVVVLPEGM
                     DPDDFIRENGQSGFQNKVNTALTYVDFKVYILKDQYDIEESKGKIDFTKEVARIIRDM
                     KSPVEQDVYIEKIASQTGVSREAIEHEVRGGQKTQKRFKERIRFEPTITKLPPAHLKA
                     EMDLIGFILMDREYFERISSRIHYEDFQDMDCRFCYRIISDS"
BASE COUNT          882 a          453 c          685 g          660 t
ORIGIN      
        1 aaatcctgta acaatgttca aatgaagcgt tccaaggggc gcttctattt tttgtttgtg
       61 aaagatattg agcttttttt ctaaaaaaga aggattttca tgacctttgt tgaatatata
      121 tatatgggaa acggaggtgg agaatgaata ttcgtgaaca gtttgaacaa agagagcgtc
      181 agacactttc atctcgagca tgcctaagta tgaacaccaa aggtcgaaag gttccagagg
      241 agaaatgtca aatcaggact gagttccaaa gggacaggga cagggtgatc cattccaagg
      301 ccttcagacg gctgaagcat aagacccagg tctttatcgc tcctgaaggg gaccatttca
      361 gaacaaggct aacccatacc ttggaggtgg cccagcttgc aaggaccatg gcaagagccc
      421 tgagactcaa cgaagacctg gtggaagcaa tttccctggc acatgatttg ggtcatacgc
      481 catttggcca tactggcgag aaggttttag acagggtgaa tccaagggga ttcaagcaca
      541 acctgcaaag tgcaagagtt gtggaactcc tggaggggtc tgagaaaaga cagggcttga
      601 atttgacata cgaggtactg gagggtataa ttaatcattc tggggataat gtgtccaaaa
      661 ccctggaagg acagatcata aagtttgcag ataggatagc atacattaat cacgatatag
      721 atgatgcaat gagggcaggg gtaataagtg aaaaagacct acctgctgat tgcattcagg
      781 tactggggaa aacccatgga gacaggatca atacaatgat agtggacctg attgaaaaca
      841 atcaggaaag tgacaggttg accatgagcg atttgattgg tgaaaagacc aacaaattgc
      901 ggcagttcat gtttgacaat gtttatctca ataaggaagc aaaaaaagag gatgacaagg
      961 ctgaatacat cattgaggtc ctgtataatt actacagaaa gaatttccac agacttccat
     1021 cggaacattt gggtatatat aagagtgtag aggtggagaa ggaggatatt atctgtgact
     1081 acattgcagg gatgaccgac aggtatatag tcaatctcta catggacctt tttgtgccaa
     1141 agccatgggg caagtactag gaagctttta ttggaattat taaaatagac aagtggtgag
     1201 agaatgtctt atgcaaatga agatgtcctt gaaaaggtca aggaccagat ggacatagtc
     1261 cagttggttg gagaatatgt tcatcttaaa aaatcgggct caaaccacat tggtttgtgt
     1321 ccctttcaca atgaaaagac tccttccttc actgtttcag agtcaaagca gattttccac
     1381 tgttttgggt gtggagaagg tggagatgga atatcctttg taatgaagaa ggaaaatctt
     1441 gattttgtgg acgcattgaa atttttagct gacaagtatg ggatcccctg ggatgaaaat
     1501 cctgacaggg acgacagccg ggaaaagagc aatctctata atatcaacaa ggaggctgca
     1561 aggtattttt acaataacct tcttaacaac aagaagccac gtgagtatct tgcaaagaga
     1621 gggatcaccg gagaaattgc aagaaagttt ggacttggct atgccactga caaatgggat
     1681 ggcctgataa agcatctact ggaaaagggc ttcacagaag atgaaatggt aaaggctgga
     1741 cttgcaggaa tcaggaagga tggatcaggg tattttgata agttcaggaa cagggtaatt
     1801 ttcccaatca ttgataccag aagccgtgtc atcggttttg gaggaagggt tctggaccat
     1861 aatttaccca aatacctgaa ttcacctgac acaatggtgt ttaacaaggg atatcacctt
     1921 tatggattga acctgcttgc aggaaacaca gataggagga gaatccttct ggtggaaggg
     1981 tatatggatg tcatatccct atatgcaggt ggtgtaaact atgcagttgc cagtttggga
     2041 acagcattga caagcaacca ggggaaactt cttaaacgat atggcgacga ggtattgatc
     2101 tgctacgaca gggatagggc tgggataaaa gccaccctca gggcaataga tgtattgagg
     2161 gagcttgagg tggatccaaa ggttgtagtt ttgccagagg gcatggaccc agatgacttc
     2221 ataagggaaa atggacaatc aggctttcaa aataaggtaa atactgcgtt aacttatgtt
     2281 gatttcaagg tatatatatt gaaagaccaa tatgacattg aggaatccaa aggaaagatc
     2341 gactttacaa aagaagttgc aaggataatc agggatatga aatctccagt ggaacaggat
     2401 gtttatattg aaaaaatagc ctcccagact ggagtatcca gggaagccat agaacacgag
     2461 gttcggggtg gacaaaagac acagaaaaga ttcaaggaga ggatcagatt tgaaccaaca
     2521 atcacaaagc tgccacctgc ccatttgaag gcagagatgg acctgatagg ctttattcta
     2581 atggacaggg agtattttga aagaatatcc agtaggattc actacgagga tttccaggac
     2641 atggactgta gattctgtta caggatcata agtgactcat
//
LOCUS       DASRQF010000144         3399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_695016, whole genome shotgun sequence.
ACCESSION   DASRQF010000144 DASRQF010000000
VERSION     DASRQF010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695016"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..104)
                     /locus_tag="VFD79_03765"
     rRNA            complement(<1..104)
                     /locus_tag="VFD79_03765"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(446..1240)
                     /locus_tag="VFD79_03770"
     CDS             complement(446..1240)
                     /locus_tag="VFD79_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004823745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HZH93196.1"
                     /translation="MANKQQHDKEKNNASEKAELKKMFRQYKDNPSRELRDVLIERHL
                     YIAEILAKKYANRGIDYDDIYQVASIGLIYAIDRYDIDKGFEFSSFATPTIIGEIKKY
                     FRDKGWTIRVPRRIQELSKKINNAKVHLSQELQRVPTVEDIAVYLNTSEEDILEAMEA
                     SKVYTPQSLDVTFDSHNDDKEVNLAELIGEDDLYFSRIENNDFLMKTMEKLNEVEKEI
                     LIERYFNKKTQVTIANNLDISQMTVSRIEKRILEKLRKEVERTLMD"
     gene            complement(1254..1610)
                     /locus_tag="VFD79_03775"
     CDS             complement(1254..1610)
                     /locus_tag="VFD79_03775"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZH93197.1"
                     /translation="MNNDIIVVTIPNKPDYTSMIRLISSSIGNKAGYNIDQIEDLKVA
                     MGEACILSYGKSSDESIQIEFEIHKDKLEVSLHWDTLGDGEESKEAALGRMIIESLMD
                     EAYYTDEEIRLIKYID"
     gene            complement(1603..1932)
                     /locus_tag="VFD79_03780"
     CDS             complement(1603..1932)
                     /locus_tag="VFD79_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004819634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZH93198.1"
                     /translation="MSLNTKVDFNYDDNQWVIKPIGEIDIYTSSVFKEEIVNAFENRK
                     ADLVIEGEHLEYIDSTGLGALISVLKMLQETEFKIYIDNIKPNIRKLFDITELDKLFV
                     IRGEGDE"
     gene            complement(2012..>3399)
                     /locus_tag="VFD79_03785"
     CDS             complement(2012..>3399)
                     /locus_tag="VFD79_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HZH93199.1"
                     /translation="LKEMLHHYLEHQKEIVTRRTQYDLTKAEDRAHIVEGLKIALDNI
                     DQIIKIIRAASDDQSAKNALMENFALSEKQAQAILDMRLRRLTGLERDKLDEEYEELI
                     KLINKYREILASERLILNVIKEELIEIKDKYGDLRRTQIKLSEDEINIEDMIEEEDVV
                     ITLTHFGYIKRMPEGTYRPQKRGGRGVIALSKKDEDFVEDLYITSTHSTILFFTNKGK
                     VYSLKAYEIPEGSRTSRGTAIVNLLQLNADEKISAIIPLYKQDPDSNLVMITREGTIK
                     KTELDQFVNIRKNGLIAISLHDGDELISVRLTDGNKDLIVVTHGGMAIRFHETDVRNM
                     GRTAMGVKAMTLNEEDAIVSMEIVEEDKHLLVISEKGFGKRTELSEYKAQNRGGKGLI
                     TYSIKEKTGEIVSAKVVSEQDEVMMITMNGTIIRLAVKDITIMGRNTQGVTLMRTKDD
                     KIVAVAMYMED"
BASE COUNT          804 a          797 c          587 g         1211 t
ORIGIN      
        1 ccaaaatcca tccgaaaatt tcattcgaaa tcacttcgct cgacttgcat gtgttaggca
       61 cgccgccagc gttcgtcctg agccaggatc aaactctcat taaaagtttg atgttagctc
      121 attatttgtt gtttaaaaaa aattgaatct ttggtttatt caaagttctc actgctgttt
      181 agttatctag gttcattacc gcactctctt gaggcgactt aattagtata ccatgtatac
      241 caatcttgtg tcaacatctt ttctgaagtt ttttatgtcg tttcctcggt ggtcctctga
      301 aaagagacaa cttgtctata atacaattaa attgtgtccc tgtcaacaca atttaggaaa
      361 ataattataa aagtatagat ttacccccat aaatagaaat ggaacctgga tttttatcca
      421 gattccataa tatatattga gggttttaat ccatcagtgt cctttcaact tccttcctca
      481 gcttctcaag gatcctcttc tcaatccttg aaacggtcat ctgggaaata tccaggttgt
      541 ttgctattgt tacctgggtt ttcttgttga agtacctttc aatgagtatt tccttttcaa
      601 cctcattgag cttctccatg gtcttcatca gaaagtcgtt gttttcaatc ctgctgaagt
      661 aaaggtcatc ctcaccgatc agctcagcca gattaacctc cttgtcgtca ttgtgtgaat
      721 caaaggttac atccagcgac tgtggagtgt agaccttgct tgcctccata gcttcaagta
      781 tatcctcctc tgaggtatta agatatacgg ctatatcctc caccgttgga actctttgta
      841 gctcctggct aagatggacc ttggcattgt ttatcttctt tgaaagctcc tggatccttc
      901 ttggaaccct tattgtccag cccttgtccc tgaagtactt cttgatctca cctatgatag
      961 ttggagtagc aaagctcgag aattcaaaac ccttgtcgat atcatacctg tctatggcat
     1021 aaattagacc tatgctggca acctgataga tatcatcgta atctatcccg cggttggcat
     1081 actttttggc taagatctca gcaatataaa gatgtctctc gatcagcaca tcccttagct
     1141 ctctgctagg gttatccttg tactgtctga acattttctt cagctctgcc ttttctgaag
     1201 cgttgttctt ttctttgtcg tgttgctgct tgtttgccat aggacaacca tccttaatct
     1261 atatatttaa tcaatctgat ttcttcatca gtataatagg cttcatccat caatgattca
     1321 atgatcatcc tgccaagtgc cgcctctttg ctttcttcgc catcaccgag agtatcccaa
     1381 tggagggata cctcaagctt atccttatga atctcaaatt caatctggat ggactcatcg
     1441 gatgatttcc cataagaaag gatacaggct tctcccatgg caaccttgag gtcctctatc
     1501 tggtcaatat tgtaccccgc cttgtttcca attgatgagg atatcaatct tatcatgctg
     1561 gtgtagtcag gcttatttgg aatggtcaca actataatgt cgttattcat ctccttcacc
     1621 tcttatgaca aaaagcttgt caagttccgt tatatcaaat agttttctga tattaggctt
     1681 aatattgtca atgtagatct tgaactctgt ttcctggagc atcttcaata cgctgataag
     1741 tgccccaagt cccgtactgt caatgtattc aaggtgttct ccttctatta cgagatccgc
     1801 tttcctgttt tcaaaggcgt ttacaatctc ctccttgaat acagatgagg tatagatatc
     1861 gatctcacca attggcttga ttacccattg gttgtcgtcg taattaaaat caactttcgt
     1921 attcaatgac attcattctt ccctccatct cgtgttattt tagttcattt tataacatat
     1981 atattgcaat gtaaaggaaa agcctcgttt cctagtcttc catatacatt gccactgcaa
     2041 ctattttatc gtcctttgtt ctcataagtg ttacaccctg ggtgtttctt cccattatgg
     2101 ttatatcctt tacggccagt cttatgattg taccgttcat ggttatcatc attacctcat
     2161 cctgctcgct aactaccttt gcggacacta tttcaccggt cttttccttt atgctgtagg
     2221 taatgagccc cttgccgccc ctgttttggg ccttgtactc agaaagctct gttctctttc
     2281 caaatccctt ttcagaaatt accaacaggt gcttgtcttc ctcgacaatt tccattgaaa
     2341 ctattgcatc ttcttcatta agtgtcattg cctttacacc cattgcagtc cggcccatgt
     2401 tcctaacatc agtttcatgg aatcttattg ccattcctcc atgggtaaca acaattagat
     2461 ccttgtttcc atcagttagt cttacgctta ttagctcgtc accatcatgt agtgaaattg
     2521 cgatcagacc attcttcctg atatttacaa actggtcaag ctcagttttt ttgatggtcc
     2581 cttcccttgt gatcataaca aggttactgt cgggatcctg cttgtacaaa ggtattattg
     2641 cagatatctt ctcatctgca ttgagctgga gcaggttaac tattgcagtt cccctggaag
     2701 tcctgctgcc ctcaggaatc tcatatgcct taagggagta gacctttcct ttgttagtga
     2761 agaagaggat tgtactgtgg gttgatgtga tgtaaaggtc ttcaacaaag tcctcatcct
     2821 tcttggaaag tgcaattacg cctcttccgc ctctcttttg aggtctgtag gtaccttcag
     2881 gcatcctttt gatatatccg aagtgagtaa gggtaattac aacatcctct tcctctatca
     2941 tgtcctctat gtttatttca tcctctgata actttatctg ggtacgtcta agatcaccat
     3001 acttgtcctt gatttcaatc agttcttctt ttatgacatt aaggatgagt ctttcgcttg
     3061 caagaatctc cctgtacttg tttattagct tgattaactc ctcgtattcc tcgtccaact
     3121 tgtccctttc aagacctgtc aaccttctaa gtctcatatc cagtattgcc tgagcctgct
     3181 tctctgaaag tgcaaagttt tccatcaagg cgttctttgc gctttggtca tcacttgcgg
     3241 ctctgattat cttaattatc tggtctatgt tatccagggc tatcttcaat ccttcaacga
     3301 tgtgggccct gtcctctgcc tttgtaaggt cgtactgagt ccttcttgtt acaatctcct
     3361 tctggtgttc aaggtaatga tggagcattt ccttaaggt
//
LOCUS       DASRQF010000145        24878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1643517, whole genome shotgun sequence.
ACCESSION   DASRQF010000145 DASRQF010000000
VERSION     DASRQF010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 24878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24878
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1643517"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1772
                     /gene="recN"
                     /locus_tag="VFD79_03790"
     CDS             93..1772
                     /gene="recN"
                     /locus_tag="VFD79_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HZH93200.1"
                     /translation="MLKELRIKNFAIIDNLTIDFTQGLNLLTGETGSGKSIIIEALEM
                     VLGGRASREMIRSGQEKAVIEAMFFLEEHLQDNLEALGYERSEILILTKEIWEDYPSV
                     SRINGRPVTLNVLKTLTRALVDIFGQHEHQSLLDTGNHMRIIDSLLPQEGKATLESIE
                     KAYSDYKELNGKYHQLTLSSQERERELDLLRFQIEEIDQAQLVEDDDDKVEQEFRRLD
                     NLKETMAEAYQALTLLGDDFQGPGAIQTLEKAIQTIFHSARYDQFLKDKGEGLQAILY
                     ELQDLYRETSDYIEGLEIDQERLFELRERLDEINSLKKKYGNKVGEILKYRDEIGERY
                     DRLLNIDKELEMVQDQLTLLRGELLDKSNKLSEIRKKVSLKLHNDMERELETLNMDNV
                     RFKVDFKDLQEPGEKGMDRIEFLISTNPGEDLKPLSKIISGGEMSRIMLAFKGILADM
                     DKMPTLVFDEIDTGISGRTAQVVGEKIRRISGGHQVISISHLPQIAALADTHFAIRKS
                     SDLKGTITKVERLKEDERIQELARLLGGVEVTETTINHAREMLQMSNKMKK"
     gene            1833..2372
                     /locus_tag="VFD79_03795"
     CDS             1833..2372
                     /locus_tag="VFD79_03795"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZH93201.1"
                     /translation="MTYEEKTMKCDRIYEGKILNLRIDTVELPDKKYSKREIVEHPGG
                     VAIVALTKDNRILLVRQYRKATDKVLLELPAGKLEVNEEPKETAIRELKEETGYTAEK
                     LEYLLECYTSPGFSSEKIYLFFGDNLEQGDQELESGEFIDVESYTVEEVKKLVDRGEI
                     MDAKTIIGIQVLVNRLAKQ"
     gene            2398..3279
                     /locus_tag="VFD79_03800"
     CDS             2398..3279
                     /locus_tag="VFD79_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZH93202.1"
                     /translation="MLRGIRDEYTRYIEKQKKLSKNTVEAYTSDIEKFSDFMDAAGIN
                     NVDMVNNTHVIRYLLQLQREGKSTSTISRHLASIRCLFEYLINNGKVKEDPTLNLKPP
                     KKEKKMPQILTEEEINRLMGLPDSTTAKGARDRALLELLYSSGLRVSEINSLKIGDIL
                     LSKGTIVLSTESGIRKVPVGSKAIGSISYYLKNYRDENNLDAPLFVNYSGKKLTRQGL
                     WKIIRTYADKVSEGNNVTPQILRNSFAAHILSHGADIKTVQEIMGHTDIASTQVYTMT
                     EREGSADDVYKKAHPRA"
     gene            3291..4592
                     /locus_tag="VFD79_03805"
     CDS             3291..4592
                     /locus_tag="VFD79_03805"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HZH93203.1"
                     /translation="MRAVDIIEKKKHKDHLTKEEIDFMVQGYTRDEIPDYQMSAFLMT
                     IYFNGMDKDEIAYLTESFVNSGEQVDLSPIQGIKVDKHSTGGVGDKISLIVIPLVASA
                     GVPVAKMSGRGLGHTGGTIDKLESIEGFRIELSSREFIDNVNKHKMAIVGQTANLTPA
                     DKKIYALRDVTSTIDSIPLIASSIMSKKIASGADAIVLDVKVGSGAFMKTIQEAEKLA
                     ETMVGIGKSLKRDTVAVITNMDQPLGHQVGNSNEVKEAIKVLSGNGSEDETTIALTIA
                     SHMCVLGGAFEDFDAAYDKLQETIRTGMAIDKLKELISIQGGNPEVVDNTDLLPGARK
                     NVDVEAEEDGFVFHIDAEAVGISAMLLGAGRRTKEDKIDYGAGITLRKKIGDPVKRGE
                     VLYTLMTNLDDYQDALQRMKEAYVISTEKPETKPYIYNIIK"
     gene            4689..5276
                     /locus_tag="VFD79_03810"
     CDS             4689..5276
                     /locus_tag="VFD79_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZH93204.1"
                     /translation="MEYLISLPGLLIAIIFHELAHGLTAYWLGDPTAKNAGRLTLNPI
                     KHIDPVGFLFMLVFRFGWAKAVPINPSYFKHRKRDTILVSIAGVTTNFIIAVITGAVL
                     VHAPIRNEILAQVLVITLWYNIMLGVFNLMPFPPLDGSKVVASLLPVKMEYYFYKYER
                     YFYLILIFLIATRGISRILGPLVDFVYRLIINLLI"
     gene            5286..6017
                     /locus_tag="VFD79_03815"
     CDS             5286..6017
                     /locus_tag="VFD79_03815"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HZH93205.1"
                     /translation="MTYNVSVEVYEGPLDLLLNLIDKNEIDIYDIPINTVTEQFIAQL
                     KKWEEMNLEVASDFIIMASTLLEIKSKLLLPKETLLVDGEEVQVDPREELVKRLMEYK
                     LYKEISENLKASEEVYSKVYYKPQEDVSDFRDPFEELSAVELKDLVDTLENILQRHRY
                     NNPQGEFFEIRREEVSLEECTREIERKVNEGVRIRFSDIISLEASRSRIIAYFLSLLE
                     LMRTRFISVRQDGSFSDLIIEKRGI"
     gene            6021..6551
                     /gene="scpB"
                     /locus_tag="VFD79_03820"
     CDS             6021..6551
                     /gene="scpB"
                     /locus_tag="VFD79_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HZH93206.1"
                     /translation="MDNRELKSIIEGLLFTFGEPIESSELAKVLDMPTKDVHSILLEM
                     KDEFDYNRRGVRIIRFNKSFQLGTRPEHHEWIKQIVQGRNTRSLSNAALETLSIVAYK
                     QPVTKADIDHIRGVKSDRSLETLIGRRLVKEAGKLDKPGRPNLYGTTDEFLRYFGLES
                     LEQLPPVDEFTIEEEE"
     gene            6578..7285
                     /locus_tag="VFD79_03825"
     CDS             6578..7285
                     /locus_tag="VFD79_03825"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZH93207.1"
                     /translation="MRLQKFMAMSGVASRRASEDLIKEGKVQVNGRVVTEMGVIVVPG
                     KDKVKVDGVNIRPESNKVYIVLNKPVGIVTSVKDEKGRAVVTDLIEEVDERIYPVGRL
                     DADTSGLILLTNDGELAYKLTHPSKMVYKRYIAIVEGVPNKGELERLRKGIRLEGRMT
                     APAKVKILKNFDEDSILDIQIHEGRNRQVKKMCEKINHPVKKLKRISFGEIELGGLEP
                     GNWRYLNDEELDYIKSL"
     gene            7340..7738
                     /locus_tag="VFD79_03830"
     CDS             7340..7738
                     /locus_tag="VFD79_03830"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZH93208.1"
                     /translation="MLSFKDVSDIDMIREFLQKNGIGDNFSQKGLFFGGFDDGKLFGT
                     SQIVLEDGKAYIQYVFIDQEYRGQGLGNAMVRSLMNKLEMNGFGMVYSKVENEYLEKV
                     GFTMDQGEYYCNLEDLFSGGCSCCSGDHHG"
     gene            7731..8318
                     /locus_tag="VFD79_03835"
     CDS             7731..8318
                     /locus_tag="VFD79_03835"
                     /inference="COORDINATES: protein motif:HMM:NF018645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZH93209.1"
                     /translation="MGDSLNNTLQFVEILMERFVSQGQVCVDATVGNGHDTAKLAGLV
                     GDEGLVYGFDIQGLAILNTEKLLKSVGLDGRVRLIQDSHENIDIHIDDEIDFIIYNLG
                     YLPGGDKSVRTESNTTIKSIDKAIKIMRQGAILLVTAYRGHQGGMAEYSSVREYFEGL
                     PQDIYNVFEFSFINQKNNPPITIGLEVRGGRTWQR"
     gene            8306..9703
                     /locus_tag="VFD79_03840"
     CDS             8306..9703
                     /locus_tag="VFD79_03840"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HZH93210.1"
                     /translation="MAKVKIMETALRDAHQSLIATRMSTEDMIPIAEKMDKVGYHALE
                     VWGGATFDSCLRFLNEDPWERLRVLRKVFKNTKLQMLLRGQNILGYKHYPDDVVEEFV
                     KKAIENGIDIVRIFDALNDLRNLETSIKAVNKYGGHAQGAISYTVSPVHNNEYYVNLA
                     KDMVEMGCDSICIKDMSGILLPYVAYDLVKDLKSAVDVPIEVHSHFTSGIANQTYMKA
                     IEAGADIVDTAISPLGNGTSQPATEPMIASLEGTEYYPDIDFDVLLEVSEYFKGLRDK
                     YLKAGLLDAKVLNVDVNTLKYQVPGGMLSNLVSQLKAQGKEDLYDEVLKEIPRVREEL
                     GYPPLVTPMSQMVGTTALFNVITGERYKMVPTETKNYVKGLYGKSPAPISDEIRKKII
                     GDDEVFEGRPADMLEPQLDNYKKEIKEYIEQDEDVLSYALFPQVAVPFFKQRLAAKYK
                     IETDLLDQENQTHPV"
     gene            9971..10171
                     /locus_tag="VFD79_03845"
     CDS             9971..10171
                     /locus_tag="VFD79_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZH93211.1"
                     /translation="MPEKNEKIVTVEKEWCKGCGICVAYCPKSVLALQDGKVEIVDLE
                     ACIKCGQCELRCPDYAIYLREV"
     gene            10174..11310
                     /locus_tag="VFD79_03850"
     CDS             10174..11310
                     /locus_tag="VFD79_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HZH93212.1"
                     /translation="MTVKLMQGNEACVAGALKAGMKFYAGYPITPSTEIAEISAEELP
                     KVGGKFIQMEDEIGGIAAALGGSLSGLKSMTATSGPGFSLKQENIGYGIITETPCVIV
                     NVQRSGPSTGLPTSPAQGDIMQARWGTHGDHSIIALYPSSVKEIYYTTIRAFNLAEKY
                     RTPVILLLDEIIGHMREKIEIPENDDVEIIDRKKPTCDPSEYKAFKVKEGDIVPEMAA
                     FGQGYRYHVTGLVHDETGFPSGNELVADKLLNRLIDKIEDNKDDIVTYEEYMLDDAEI
                     AIVSFGSIARSSKTAVDTLRSQGYKVGMFRPITIWPSPEEQLLELGKKMKKIYVVELN
                     AGQYFLEVDRIAGKHTEVGKYGKINGDIITPEEIVAVVKEDQNA"
     gene            11303..12124
                     /locus_tag="VFD79_03855"
     CDS             11303..12124
                     /locus_tag="VFD79_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HZH93213.1"
                     /translation="MPSEIVKNYFRTQNLPHIWCPGCGNGIVTRALVKAVDALGLDKD
                     KVCVVSGIGCSSRAPGYLDFNTLHTTHGRALAFATGVKMANPELKVIVVTGDGDSSAI
                     GGNHLIHACRRNIDITTIVFNNNTYGMTGGQFSPTTPTGDKGTTAPYGSIDPNFDLCN
                     LATAAGATYVARGTVFAANQLEKNIIAGIQNKGFSFIEAMSTCPTYYGRKNRKGSAVD
                     MTTYLKESAINKVAWDKLPDEKKEGKFVIGELHSIERPEYTEEYDKLIASVRKEG"
     gene            12126..12653
                     /locus_tag="VFD79_03860"
     CDS             12126..12653
                     /locus_tag="VFD79_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HZH93214.1"
                     /translation="MKRWELRFSGSGGQGLITGGIILAEAAILDGKNALQSQSYGPEA
                     RGGASKAEVIISEEEISFPKVEHCDLLLSLTQLSCDKYLDSLKSGGILVIDDSVESPD
                     RDDIKIYRIPILSTASEKLGKPMVANIVALGAIHSITGIVSRESLQNAVLSRVPKGTE
                     ELNKTALKEGYELVK"
     gene            12669..13535
                     /locus_tag="VFD79_03865"
     CDS             12669..13535
                     /locus_tag="VFD79_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HZH93215.1"
                     /translation="MVPSQNVIKNIQDNYSKLSKGQKIIAEYMINQYDKAAFMTAASL
                     GKNLNISESTVVRFANALGYSGYKELQRELQELIRNKLTTVQRLNLSENYSKNEHTIA
                     KVMEKDVENLRKTLSEVDFDKFYTAVEMTTKARNIYIMGLRSSSFLSGYMAFYMNFIF
                     DNVKLVQPGANDIFEQLAKASDEDVIICLTYPRYSKRTLEALEYSKNKGCRIVSFTDS
                     LISPAADKSDITIIARSDMLSFVDSLVAPMSLINAFIIAVGSEKKEDITPYFEELEKV
                     WKKYSIYDENSM"
     gene            13709..14929
                     /locus_tag="VFD79_03870"
     CDS             13709..14929
                     /locus_tag="VFD79_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZH93216.1"
                     /translation="MKLKIGVIGGGPSGIMAAGAAAEKGHDVVLFEKNNQLGKKLFIT
                     GKGRCNITNAAPIDEFFDNIVTNKNFLYSSLYTFDNNAIIELLSKYGLTTKVERGNRV
                     FPESDKSSDVIKAFKRYLDRNGVDIRLNSDVSHIKYDGEIFTVFIRDREEYKCDKLII
                     TTGGKSYPATGSTGDGYLFAKSLGHTVTDLRPSLVPIELNDEWVKDLMGLSLRNVTLT
                     AYKGKKQVHSEFGEMVFTHYGISGPIVLSMSNYVNSIKSNMSLTLDLKPALDEEKLDA
                     RIVRDFALFSNKHLKNALNELLPQRLIPWVIEASGIYPEKVVHQITKEEREKLVRALK
                     NFPMHFKGFRPIEEGIVTSGGINVKEINPSTMESKIIPGLYFAGEIIDIDALTGGYNL
                     QIAYSTGYLAGINV"
     gene            15235..15897
                     /gene="cmk"
                     /locus_tag="VFD79_03875"
     CDS             15235..15897
                     /gene="cmk"
                     /locus_tag="VFD79_03875"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HZH93217.1"
                     /translation="MSENCSIAIDGPAGAGKSTVARIVANKLSFEYIDTGAMYRAYTL
                     KVLQNNLDPKNQDEVNSLLDSTDISFSNNHIYLDDKLVDNEIRDKSISDNVSYIASSK
                     DVRERMVSLQQEMASRKSVVMDGRDITTVVLPNADYKFFVTASVEERGRRRYLELVDK
                     GLQNITLDGIIDDIRRRDEIDSTREESPLTRTEDSILVDTTDLSIEETVDLIINLVKG
                     GK"
     gene            15900..16478
                     /locus_tag="VFD79_03880"
     CDS             15900..16478
                     /locus_tag="VFD79_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZH93218.1"
                     /translation="MFYSLVKAIAFVVFNIVFRIKVEGKQNIPGDGRIVLCSNHKSNW
                     DPLILAAMFPRKISWMGKKELFENKFLKVVLNWLGVFPVDRQVADLSAVKTALRILKS
                     EKVLGIFPEGTRVTEYNPENAKAGVALLAMKSQAPVLPVYIEGSYKLFSRLEIVIGEP
                     MEYHKKYTGRLGNEEYSKISQEILAKIYKLGG"
     gene            16482..18473
                     /locus_tag="VFD79_03885"
     CDS             16482..18473
                     /locus_tag="VFD79_03885"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase/30S ribosomal protein S1"
                     /protein_id="HZH93219.1"
                     /translation="MEIILAENAGFCFGVDRAVRMTEEELSKTDSSVYSYGPLIHNPQ
                     AVKALEAKGLETIDRFEDISEGRIIVRSHGVPEETINDIKELGLHLVDCTCPYVLAVH
                     KKVRDYSSKGYSIVIIGDKDHPEVVGINGWCENQAHIVNSAEDAKALPDLEKSCVVSQ
                     TTNRLDKFLELSGIVEEKSQETVLFNTICNATKLRQEAAEEVAKKVDAMIVIGGKHSS
                     NTIKLAEISSRHCKNVFHIETIEEFALQRLQNFNTIGITAGASTPDWIIKEAVKVMEN
                     YNKDEMMEAIESSFKRINRGDVLKGTVLYVTNNEVMVNINYKSDGIIDRSEISKEQDA
                     DPKELFKVGDEIDVYVMKLDDGEGNVVLSAKRVDFIKDWEILEESFKNEEILEVKVLN
                     AVKGGLTVLVSGVNGFMPASQISMNYVSDLNQFKGQTLNAKIIDFDIQKRRMILSRKA
                     VEKVEVEKKRAELWETIEEGKMLTGVVQRLTDFGAFVDLGGIDGLIHISDLAWFRVKH
                     PSEVLKEGDNVEVKVLAFDKEKNRISLGLKQTTEEPWEVFLKDYKVGDVIDGEVVNIL
                     DFGAFVRVIKGVDGLLHVSQISKDHVEKPSDVLKVGDKVTVKITEINEADKKISLSAK
                     DAIETDEPAEEAPAVEEEAEETYEQAEFNTSVEELTKED"
     gene            18553..19605
                     /locus_tag="VFD79_03890"
     CDS             18553..19605
                     /locus_tag="VFD79_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HZH93220.1"
                     /translation="METKELLEILSNGNGVSGFEFNLKNEVINAFDRYADSVQVDKLG
                     NIIAHKRGSGDGSVKILMAAHMDEIGFMVKDIDKKGFIHFTNIGGIDPRTILAQEVVI
                     HGREEVPGVIGSKPPHLQDESEQEKSIKMEDMIIDTGYEKKDLDQLVSIGDSITVGRK
                     MVSLLNSRVAGKAMDDRAGLAALFEAMKELQNINHYPDIYFVATIQEEVTMAGALTAT
                     YGINPDIGIAVDVGFGRTPELNSSNSIEMGKGPGITLGGNIHPGLRKRLVSVANDYNI
                     PIQTDVRPGPTGTDARAMQITREGIPALVLSIPLRYMHTSVEVLDLKDVSHTAKLLAF
                     FSASIKENEMEGLLCF"
     gene            19596..20618
                     /locus_tag="VFD79_03895"
     CDS             19596..20618
                     /locus_tag="VFD79_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HZH93221.1"
                     /translation="MLLKELTEASGVSGNEKEVRDLIKSTLEGYVDHMKTDGIGNLIV
                     YKKGSKPGGRKLMIAAHMDEVGLIVKDVDSSGLLKFMPVGGIDKRVLVSKAVKVGQQK
                     LPGVIGAKPIHLQKRNEWSKVLDMDDLYIDIGASSKEEAEKQVSIGDYVAFDTPYMEM
                     GNGTIKAKALDDRVGCSILIDLLKSDIDAEIYGAFTVMEEVGLVGAGPAAYQIKPDMA
                     IVLEGTLCYEIEDLDSHEMPTIMGQGPAISLIDRTTVYDRSLRENVVRIAEENSIPYQ
                     YRKTSMGGNDSGKIHITGTGCATTGISVPTRYIHSPLSLISKSDYHNTFKLVEAIVKE
                     FEKGAI"
     gene            20619..21611
                     /locus_tag="VFD79_03900"
     CDS             20619..21611
                     /locus_tag="VFD79_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HZH93222.1"
                     /translation="MNFNVELLEKLVSVYGPSGDESLISATIRQEIGGYVDEVTTDAL
                     GNLIARKKGDGKKIMVSAHMDQIGFIVTDIDKEGFLRFGNIGGISPDISLSQRVIFKN
                     GTVGAIFSEPLDKPSERKLENMYIDIGAKDREEAEKMVSVGDMCIYYSPFSHNENVVF
                     TPYLDDRVGCFVAIEALKRITTTENDIYFVFSVQEEVGLRGAKTAAYGIEPDFGIAVD
                     VTAHGDTPKAKRFAIGLHKGAAIKVKDNSIITHPSVRNSLIDIAKAKGIAYQLEVLEF
                     GGTDSGAIHVTKTGVPSGVISIPTRYVHSTVEMASKEDIESSISLLEGYLSKKL"
     gene            21735..23054
                     /gene="miaB"
                     /locus_tag="VFD79_03905"
     CDS             21735..23054
                     /gene="miaB"
                     /locus_tag="VFD79_03905"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HZH93223.1"
                     /translation="MKKYLINTSGCQANELDSEKISWVLDTMGYEPTNDKEEADIILF
                     NTCAVRKSAEDKVFGQLGELKELKRRKPEVMLGICGCMMQREDIRDFFLSKHRHIDLV
                     FGTNNIHKLPQLISRQLETGKTIVDIIEDTREIDENIDAHRKYSYKAFVDIMYGCDNY
                     CTYCIVPYTRGRERSREPESILQEIRKLVRSGVKEVTLLGQNVNSYGKTLSMDYTFPD
                     LLRDVNDIEGIERIRFMTSHPKDFTEGLIKAYVELDKLSPHLHLPVQSGSDRMLKMMN
                     RKYTRGEYLEKVKAIKERVPNLAITTDIIVGFPGETEEDFQETIDLVKEVEFDSAYTF
                     LYSIREGTIAAKIEDQVPDKIKHVRFQRLLDTLNPIGLKLNQKLLGTVQRVLVEEVSK
                     NDSKVMSGRTESAKLVHFPGGDELIGKIVNVRIENVKTFTLEGEMID"
     gene            23064..>24878
                     /gene="mutS"
                     /locus_tag="VFD79_03910"
     CDS             23064..>24878
                     /gene="mutS"
                     /locus_tag="VFD79_03910"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HZH93224.1"
                     /translation="MGTLTPMMQQYVDTKKKYQDALLFFRLGDFYEMFFEDAKIASRE
                     LEIALTSRDCGLDEKAPMCGVPHHVAETYVSRLVDKGYKVAICEQVEDPALAKGLVKR
                     DVVRIVTPGTIMDGNLLNEKSNNFLASIFLDEYGVGLSYVDNSTGEMFTTEFIGEEEN
                     SQRFVLDELGKVMPSEIVCNKGFAKDKKLVADIRNKINPYINVQDDASIDVETSRESI
                     LGFLSEEDYEKNSLKEKIYSILSLGRLVDYLRDTQFNAIDHIKNLQYYQPDHYMLMDI
                     NTRANLEIHETMRSKEKRGALIGILDKTKTAMGGRLLRKWLEQPLLDKKQIESRLDMV
                     EYLCNNPFQLEEIKDILKTIYDIERLISKISNGNPGGRDMLALSQSIQNIPQLKGILS
                     ETGLAQFHRLQEEMDDLMDIHQVLTSAIDENPPLSVKEGGIIRQGYNEHLDDLRESKT
                     RGKEWLARFESDEKTRTGIKTLKVGYNRVMGYYIDIRKSSIDSVPENYIRKQTLANSE
                     RYYTTELKEIEDKILGADEKAIQLEYELFQEIRNQIKEEIKRLQKTSKHIAVLDVVMS
                     LAIVANANGFTRPSLNTKGIINIEEGRHPVVEATIKHQL"
BASE COUNT         8125 a         4443 c         6256 g         6054 t
ORIGIN      
        1 ctaaaaaagg tagaggcagg catggatgaa agggagatcg accaggttat tgaagaggca
       61 cacgaaaacc ttaaatagat ggaggtaggc ttatgctcaa ggagctaagg attaagaact
      121 tcgcaattat tgataatctg acaattgatt ttacacaagg cctaaacctg cttacaggtg
      181 aaacagggtc cggtaagtcc attataatcg aggcacttga aatggttctc ggtggaaggg
      241 caagccggga aatgattcgt tcaggacagg aaaaggcagt aattgaggcc atgttctttc
      301 tggaggaaca ccttcaggac aacctggagg ctctgggtta tgaaagaagt gaaatcctga
      361 ttctcaccaa ggaaatctgg gaggactatc caagcgtttc aaggatcaac gggagacctg
      421 tgaccctaaa tgtacttaag accctcacta gggctttggt tgacatattc ggccagcatg
      481 aacaccagtc ccttctggat actggaaacc acatgcggat tatcgactcc ctgctgcccc
      541 aggagggcaa ggcaaccctt gagagtatag agaaagcata cagtgactac aaggaactca
      601 atggaaagta ccaccagctt accttgtctt cccaggaaag ggaaagggag ctggaccttt
      661 tgaggtttca gatagaggaa atcgaccagg cacagctggt ggaggatgac gatgacaagg
      721 tggagcagga gttcaggagg cttgacaatc tcaaggagac catggctgaa gcctaccagg
      781 ccctaacact acttggggat gattttcagg gtcctggtgc aattcagacc cttgagaagg
      841 ctatccagac aatatttcat tcagccaggt atgatcagtt cctaaaggac aagggggaag
      901 gactacaggc aatcctatat gagctccagg acctttacag ggagaccagc gactatatcg
      961 aaggacttga aatagaccag gaaaggctct ttgaacttag ggaaagactt gatgaaataa
     1021 actcccttaa gaaaaagtat gggaacaagg taggggagat cctaaaatac agggatgaaa
     1081 ttggggagag gtatgacagg ctcctaaaca tagacaagga acttgaaatg gtccaagatc
     1141 agctaactct acttagaggg gagcttttgg acaagtccaa taagcttagt gaaataagaa
     1201 agaaggtttc ccttaagctt cacaatgaca tggaaaggga gcttgagaca cttaatatgg
     1261 acaatgtaag gttcaaggtg gactttaagg accttcaaga gcctggtgaa aagggtatgg
     1321 acaggattga gttcctgatt tcaaccaatc cgggggaaga ccttaagccc ttgtcaaaaa
     1381 tcatctcagg tggagaaatg tccaggatca tgctggcctt caaggggatt ttggctgata
     1441 tggataaaat gccaaccctt gttttcgatg agattgatac aggcataagc ggccgaactg
     1501 cacaggttgt tggtgaaaag atcaggagga tttcaggggg acaccaggtg atttcaattt
     1561 ctcatctgcc tcagattgca gcccttgccg atacacactt tgccatcaga aaatcatctg
     1621 atttaaaagg caccataacc aaggtggaga ggctaaagga ggatgaaagg atccaggagc
     1681 tggccaggct gctgggtggt gtggaggtaa cagaaaccac cataaatcac gcaagggaaa
     1741 tgctccaaat gtcaaataag atgaagaaat agggcatata tgctataatc tatgtataaa
     1801 taaagccagt aataaaaatt agggggatct gtatgactta cgaagagaaa accatgaagt
     1861 gtgacaggat ttacgaggga aagattttaa accttagaat agacacagtg gagcttcctg
     1921 acaagaaata ttccaaaagg gaaattgttg aacatccagg tggcgtggcc attgtcgcac
     1981 tgacaaagga taacaggata ctgttggtaa ggcagtacag aaaggcaacc gacaaggtgc
     2041 ttcttgagct acctgcaggc aagctggagg taaatgagga gcccaaggaa actgctatca
     2101 gagagttgaa ggaagagacg ggatacacgg ccgagaagct tgagtacctg ctggaatgct
     2161 atacctcgcc gggattctcc agtgaaaaga tatacctttt ctttggggac aaccttgagc
     2221 agggtgacca ggagcttgaa tcaggtgaat tcatagatgt tgaaagctat acggttgaag
     2281 aggtgaagaa gctggttgac aggggagaaa taatggatgc caagaccata atcgggatac
     2341 aggtcctggt taatcgactg gcaaaacaat aaaaatatac ttaaaagggg atagaatatg
     2401 ttaaggggaa taagggatga gtacaccagg tatatagaaa aacaaaaaaa actcagtaaa
     2461 aacactgttg aggcatatac aagcgatatt gaaaagttca gtgattttat ggatgcggca
     2521 ggaatcaaca atgtggacat ggtaaacaac acccatgtga tacgttacct cctgcagctt
     2581 cagcgtgaag gaaaatcaac atccactatc tccagacatc tagccagcat acgatgtcta
     2641 tttgagtacc tgataaataa tggcaaggtc aaggaagacc ccaccttgaa tctgaagcca
     2701 ccaaaaaagg aaaaaaagat gccccagatt ttgactgaag aggaaataaa caggcttatg
     2761 ggcctgcctg actccacaac agctaagggt gcaagagaca gggctctttt ggagcttctc
     2821 tattcctctg gcctgagggt gtctgagata aattccctta aaattggtga catactcctt
     2881 tcaaagggta caatagtact gtcaactgag tcgggaataa gaaaggtccc tgttgggtca
     2941 aaggcaattg ggagcatctc atactatcta aagaattaca gggatgaaaa caatttggat
     3001 gcacccctgt ttgtgaatta tagcggtaag aaacttacaa gacagggtct ttggaaaatc
     3061 ataaggacct atgccgacaa ggtttctgaa gggaataatg taacccctca aatattgagg
     3121 aactcatttg cagcccacat acttagccat ggtgcagata tcaaaaccgt acaggaaatc
     3181 atgggccata ctgatattgc ttccacccag gtctatacaa tgaccgagag ggaaggaagc
     3241 gcggatgatg tgtacaagaa ggctcaccca agagcttagg aggattagaa atgagagctg
     3301 ttgatataat tgaaaaaaag aaacacaagg atcatttgac caaggaagaa attgacttta
     3361 tggtccaggg ttatacaagg gatgaaatac ccgactacca gatgtcagcc tttctgatga
     3421 caatatattt caatggtatg gacaaggatg agattgcata tttaacggag agctttgtta
     3481 attccggtga gcaggttgac ctttcaccaa tccagggaat aaaggttgac aaacactcca
     3541 ccggtggggt tggggacaag ataagcctaa tcgtcatacc cttggttgcc agcgcaggtg
     3601 tgcctgttgc caagatgagt ggaaggggcc ttggccatac aggtggcacc attgacaagc
     3661 tggagtcaat agaaggcttt aggattgagc tttcatcaag ggaattcatt gacaatgtaa
     3721 acaagcacaa gatggcaata gtcggtcaaa ctgcaaacct tacacctgct gacaagaaga
     3781 tttacgcatt gagggatgta acctccacaa ttgacagcat tccacttatt gccagctcaa
     3841 tcatgagcaa gaaaatcgcc tctggagcag atgcgatagt tcttgatgtg aaggtggggt
     3901 caggtgcatt tatgaagacc atccaggagg ctgaaaagct agcagaaaca atggttggaa
     3961 taggaaagag ccttaagagg gatactgtcg ctgttatcac aaacatggac cagccccttg
     4021 ggcaccaggt tggaaacagc aatgaggtaa aggaggcaat caaggtcctg tctggaaatg
     4081 gctctgagga cgaaaccact atcgccttga ccatagcttc acacatgtgt gttctgggag
     4141 gagccttcga ggattttgat gcagcctatg acaagcttca ggagacaatc aggaccggga
     4201 tggcaataga caagctcaag gaacttattt ccatccaggg gggaaaccca gaggtagtgg
     4261 ataacacaga ccttttgcca ggggcaagga agaatgtgga tgttgaagcg gaggaggatg
     4321 gctttgtgtt ccacattgat gctgaggctg ttggcataag tgcaatgctt ctcggtgcag
     4381 gaagaagaac caaggaggat aagattgact acggcgcagg aataacctta agaaagaaga
     4441 ttggagaccc tgtaaagagg ggagaggttc tatacaccct aatgacaaat cttgatgact
     4501 atcaggatgc cctccaaagg atgaaggaag cctatgtaat ttccactgaa aaaccggaga
     4561 caaaacctta tatttataat ataataaagt aagtatgcat atgatacctc gacaagaaca
     4621 gtcggggttc attattatag tagaagatat tgaaacaagt ggacataatt tgaaagtaga
     4681 ggaaagctat ggaatattta atcagtctac cagggctgct gatcgctatt atctttcatg
     4741 aactggccca cggtctcaca gcctactggc ttggagaccc gacagccaag aatgcaggga
     4801 gactaacctt aaatcccatc aagcatattg atccggtggg gtttttgttt atgttggtat
     4861 tcagatttgg atgggccaag gccgtaccca tcaaccccag ctactttaaa cacaggaaaa
     4921 gggacacgat acttgtctcc atagcagggg tgaccaccaa cttcataata gccgtaataa
     4981 cgggggcagt gctggtccat gccccaatca gaaatgagat tctggcacag gtgctggtca
     5041 taaccctttg gtacaatatt atgctggggg tctttaacct aatgccattt ccaccccttg
     5101 acgggtcaaa ggttgttgca agcctcctgc ctgttaagat ggaatactac ttctacaagt
     5161 acgagaggta cttctacctg atactcatat tccttatagc aacaagggga ataagtagga
     5221 tacttggacc attggttgat tttgtctacc gattgattat aaatctgcta atctaggagc
     5281 atgccatgac ctataatgta agtgtggagg tttacgaagg tcctttggat cttcttttga
     5341 acctgataga taaaaatgaa atagacatct acgacattcc aataaacact gttactgaac
     5401 agtttattgc ccagctgaaa aagtgggagg aaatgaacct tgaggtggcc agtgacttta
     5461 taatcatggc atcaacattg ctggaaataa agtcaaagct cctcctgcca aaggaaaccc
     5521 ttctggtgga tggagaggag gtccaagttg acccaaggga ggaacttgta aaaaggctta
     5581 tggaatataa gctctacaag gaaatctccg aaaaccttaa ggcctcagaa gaggtttaca
     5641 gcaaggtcta ctacaagcca caggaggatg taagtgactt cagggacccc tttgaggagc
     5701 tgagtgcagt ggaacttaag gacctggtgg acacccttga aaacatactc caaagacatc
     5761 gctacaacaa cccacaggga gaattcttcg aaatcagaag agaggaagtg agccttgagg
     5821 agtgtacaag ggaaatcgaa aggaaggtca acgaaggggt taggataagg tttagtgaca
     5881 taatctctct agaggcatca agatccagga taatagccta ctttctgtcc ctcctggagc
     5941 taatgaggac caggtttata tccgtaaggc aggatgggag cttttcagac cttattattg
     6001 agaagagagg tatttgatag atggacaata gggaattgaa gtcaattatt gaaggcttgc
     6061 tctttacctt tggagagcct attgaaagca gcgaactggc caaggttttg gatatgccca
     6121 caaaggatgt ccattcaatt ttactggaga tgaaggatga gtttgattac aatagacgtg
     6181 gtgtaaggat cattagattc aacaagtcct tccagctagg cacaaggcct gagcaccatg
     6241 agtggataaa acagattgta cagggcagaa atacaaggtc tctttcaaat gctgccttgg
     6301 aaaccctgtc aattgtggcc tacaagcagc ctgtaacaaa ggctgacata gaccatatta
     6361 ggggagttaa aagcgacagg agccttgaga cactcattgg gagaaggctg gtcaaggaag
     6421 cgggtaagct tgacaagcca ggaagaccta atctgtatgg aactacagat gagtttctca
     6481 ggtattttgg actggaatca cttgagcaat taccaccggt ggatgagttt actatagaag
     6541 aagaggaata gaggagttaa ttatgaagga taagggtatt agattgcaaa aattcatggc
     6601 aatgagcggt gtggcctcca gaagggcttc agaagacctt attaaggaag gcaaggttca
     6661 ggtcaatgga agggtagtaa ctgaaatggg agtcattgtg gttcctggca aggacaaggt
     6721 caaggtggat ggggtgaaca taaggccgga aagcaacaag gtctatattg ttctaaacaa
     6781 gcctgttgga attgtaacca gcgtcaagga tgaaaaggga agagccgttg tcacggacct
     6841 gattgaggaa gtggatgaaa gaatctaccc tgtgggaagg cttgatgcgg ataccagtgg
     6901 cctgattctt cttaccaacg atggggagct tgcctacaag ctgacacatc ccagcaaaat
     6961 ggtatataaa aggtacatcg caattgttga aggcgtaccc aacaagggag agctggaaag
     7021 gctcagaaag gggataagac ttgaaggtag aatgactgca cctgccaagg taaagatcct
     7081 taagaacttc gacgaagact caattttgga tatacagatt catgagggaa ggaacagaca
     7141 ggttaagaag atgtgtgaaa agatcaacca tcctgtgaaa aagcttaaga ggatatcatt
     7201 tggggaaatc gaactgggcg gtttggagcc gggcaactgg agatacctca atgacgagga
     7261 gctggactat ataaagtccc tttaggctta ggtggctata atggaatcag catgtaatat
     7321 gatttgaaga ggtggactta tgcttagctt caaggatgta tcggatatag atatgattag
     7381 ggagtttctc caaaagaacg gtatagggga taacttcagc caaaagggtc tttttttcgg
     7441 tgggtttgac gatgggaaac tctttggtac aagccagata gtcctggagg atggaaaggc
     7501 ttacattcag tatgttttca tcgaccagga gtacaggggt caaggccttg gtaatgccat
     7561 ggtaaggtcc cttatgaaca agcttgaaat gaatggcttt ggaatggttt attctaaggt
     7621 tgaaaatgag tatctggaga aggttggctt caccatggac cagggagaat attactgtaa
     7681 tctagaggac cttttttctg gtggatgctc ctgctgctcg ggagaccacc atgggtgata
     7741 gtttaaacaa tacactacaa tttgtggaga ttctcatgga aagatttgta tcccagggac
     7801 aggtctgtgt tgatgcgaca gttggaaacg gtcatgatac cgccaagctg gcaggtcttg
     7861 taggggatga aggtcttgta tatggctttg acatccaggg ccttgccata ctcaatacag
     7921 agaagctttt gaaaagtgtg ggattggatg gcagggtaag gcttatccag gacagtcatg
     7981 agaatattga tatccatatt gatgatgaga ttgattttat aatctataat ttagggtatc
     8041 taccaggagg agacaagtcc gtaaggactg aatcaaatac gacaatcaag agtattgaca
     8101 aggcaataaa gatcatgagg caaggtgcca tactgctggt gacagcatac agggggcacc
     8161 agggaggaat ggctgaatat tcatctgtaa gggaatattt tgaaggtctt ccacaagata
     8221 tctacaatgt atttgagttt tcatttatca atcagaagaa caatccccca attacaattg
     8281 ggttggaagt cagaggaggt agaacatggc aaaggtaaag attatggaaa ctgctttaag
     8341 ggacgctcac cagtcactta ttgcaacgag aatgagcact gaggatatga tccccattgc
     8401 agaaaagatg gacaaggttg gttatcatgc attggaggta tggggaggcg ctacatttga
     8461 ctcatgcctg agatttctta atgaagaccc atgggaaaga ttaagggtac taagaaaggt
     8521 attcaagaac accaagcttc agatgctgct gagaggacaa aatatcttgg gctacaagca
     8581 ctatcctgat gatgttgtgg aggagtttgt aaagaaagcc atcgagaatg gaattgacat
     8641 cgtaaggatt tttgatgcat tgaatgattt aagaaacctt gaaacctcaa ttaaggctgt
     8701 caataaatac ggtggacatg cgcagggtgc aatatcatat acagtgagcc cggttcacaa
     8761 caatgaatac tatgtaaacc tggccaagga catggtcgaa atgggctgtg attccatctg
     8821 tatcaaggac atgtcgggta tactacttcc atatgtggcc tatgacctgg tcaaggacct
     8881 taagtcagct gttgatgtac caatagaggt tcactcacac tttacaagtg gtatagccaa
     8941 tcagacctac atgaaggcaa ttgaggctgg agccgatatt gtggatactg caatttcacc
     9001 acttggaaac ggcacaagtc agccggccac agagccaatg atagcatcac ttgagggtac
     9061 agaatactat cctgatatag atttcgatgt gcttcttgag gtatcggagt acttcaaggg
     9121 tctgagggac aagtatttga aggctggtct tttggatgca aaggtcctta atgtcgatgt
     9181 caatacactt aagtaccagg tgccaggtgg aatgctgtca aacctagtat cccagcttaa
     9241 ggcacagggc aaggaagacc tttatgatga ggtattgaag gagatcccaa gggttaggga
     9301 agagcttgga tatccgccac ttgtgacccc aatgagccag atggttggaa caacagcact
     9361 tttcaacgtc attaccggcg aaaggtacaa gatggttcca acagaaacca agaactacgt
     9421 gaagggcctt tatggaaaat cccctgcacc aatctctgat gaaattagaa agaagataat
     9481 tggggatgat gaggtatttg aaggtagacc ggctgatatg cttgagccac agctggataa
     9541 ctacaaaaag gaaataaagg aatatattga gcaggatgag gatgtgctaa gctatgcatt
     9601 attccctcag gttgcagttc cattcttcaa gcagagactg gcggccaaat acaagattga
     9661 gacagatctt ctggatcagg aaaaccaaac ccatcctgta tagttataaa atcaatggac
     9721 tagaaaaagc aagcctcagg gcttgttttt ttgcggtaaa aatggggaat cagtatggga
     9781 aaatcttatt gaccaatgtc catatattca atcgtttgaa agcaaacttg caaaaacctt
     9841 tcaaagtggg taaattatga aataaatctt gcaaaaccat atatactttg ttattattta
     9901 ggtaagaaat caagttgcct atttgataat atttaggtga gtagtcaagt tacctatagg
     9961 agggaaaacg atgccagaaa agaatgaaaa gattgttacc gttgagaagg aatggtgcaa
    10021 gggctgtggc atttgcgtag catactgtcc aaagagcgtt cttgctttac aggatggcaa
    10081 ggtggagatt gttgatcttg aagcttgcat aaaatgtggt caatgtgaat tgcgttgtcc
    10141 tgattatgcg atttacttaa gggaggtata aagatgactg taaaattaat gcagggaaat
    10201 gaagcctgcg tcgccggagc acttaaggcg ggaatgaagt tctatgctgg ttatcccatt
    10261 acaccttcaa ctgaaatagc tgagatttca gctgaggagt tgcctaaggt tggaggaaag
    10321 ttcatacaga tggaagatga aatcggtgga attgcagcgg ctttgggagg ttcactttcc
    10381 gggctcaagt caatgactgc cacatcaggc ccaggtttca gccttaagca ggagaacata
    10441 ggctatggaa tcattacaga aaccccatgt gttattgtta acgttcagag aagcggacca
    10501 agtaccggac ttcctacatc acctgcacaa ggggatatca tgcaggcccg ttggggaact
    10561 cacggtgacc actcaataat tgcattatat ccatcctcag taaaagaaat atactacaca
    10621 accattagag ccttcaatct ggcagaaaaa tatagaacac ctgtaatact gttactggat
    10681 gaaataattg gtcacatgag ggagaaaata gagattcctg aaaatgatga tgttgaaata
    10741 atcgacagga aaaagcccac ttgtgatccg tcagaataca aggcattcaa ggtaaaggaa
    10801 ggtgacatag ttccggaaat ggctgctttc ggtcagggct acaggtacca tgtaactgga
    10861 cttgttcacg atgagacagg attcccatca ggtaatgaac tggttgcaga caaactactt
    10921 aacagactga tagataagat tgaagacaac aaggacgata ttgtaaccta tgaggaatac
    10981 atgcttgatg atgctgaaat agcaattgtt tcatttggat caattgccag gtcatcaaaa
    11041 acagctgtgg atacacttag gagtcagggc tacaaggtag gaatgttcag accaattacc
    11101 atctggcctt caccggaaga acagctactg gagcttggca agaagatgaa gaaaatctat
    11161 gtggttgaac tgaatgctgg ccagtacttc ctggaggttg acaggattgc agggaaacat
    11221 acagaggttg gcaagtacgg caaaatcaat ggagatataa tcactcctga ggaaatagta
    11281 gcagttgtga aggaggatca aaatgcctag tgaaatagta aaaaattatt ttagaaccca
    11341 gaatttacct catatttggt gtcctggctg cggaaatggt attgttacta gggcattggt
    11401 taaggctgtt gatgcacttg gtcttgacaa ggataaggtt tgcgtagttt caggcatagg
    11461 ctgttcatca agagcaccag gctatttgga cttcaacaca ctccatacca cacatggtag
    11521 ggctttggca tttgcaactg gagttaaaat ggcaaatccg gagttgaagg tgatcgtggt
    11581 cacaggagac ggtgactcat ctgcaatcgg aggaaaccac ctgattcatg cctgcagaag
    11641 gaacatcgac attacaacaa tcgtattcaa caacaacacc tacggaatga ccggaggaca
    11701 gttctcacct acaacaccaa caggcgacaa gggtacaact gcaccttatg gaagcattga
    11761 cccgaacttt gacctgtgca atcttgccac tgcggctgga gccacatatg ttgcaagagg
    11821 aactgtgttt gcagcaaacc agcttgagaa gaacataatt gcaggaatcc agaacaaggg
    11881 attctcattt attgaagcca tgtcaacctg tccaacctac tacggaagaa agaacagaaa
    11941 gggtagtgcg gttgatatga caacatacct gaaggaaagt gccatcaaca aggttgcttg
    12001 ggataagttg cctgatgaga agaaggaagg caagtttgtc atcggggagc ttcatagcat
    12061 agaaagacca gagtacacag aagagtacga taaactgatt gcatccgtaa ggaaggaggg
    12121 ctaatatgaa acgttgggaa ttgagatttt caggatctgg aggacaggga ctgataactg
    12181 ggggaattat tcttgctgaa gctgccatac tggatggtaa aaatgccctt caatcacagt
    12241 catacggacc tgaggcaagg ggtggagcca gtaaggctga agtaattatc agtgaggaag
    12301 agatatcatt tccaaaggtt gaacattgtg accttttgct atcattgaca cagctgtcct
    12361 gtgacaagta tcttgactct cttaaatcag gtggaatact tgtaatagat gactccgttg
    12421 aaagtccgga cagggatgac attaaaatat acaggattcc aatactatca actgcttctg
    12481 aaaagctggg aaagccaatg gtagccaata ttgttgccct tggtgcaatc cacagcataa
    12541 cagggatagt ttcaagggaa tcgcttcaaa atgccgttct ctcaagggtt cccaagggaa
    12601 ctgaagagct gaacaagaca gcccttaaag agggatacga attagtcaag tagagaggtt
    12661 gttgcccaat ggtaccgagt cagaatgtaa taaagaatat ccaggacaat tactcaaagc
    12721 tgagcaaggg tcagaaaatc atagccgagt acatgataaa ccagtacgac aaggctgcct
    12781 tcatgactgc tgcaagtttg ggtaaaaacc tgaatatcag tgagtcaaca gttgttaggt
    12841 ttgccaacgc ccttgggtat tctggctaca aggaattgca gagggagctt caggaactaa
    12901 taagaaacaa gctgacaact gttcagagac tgaacctttc agaaaattac tcaaaaaacg
    12961 aacatacaat tgccaaggtt atggaaaagg atgttgaaaa ccttagaaaa accttatcgg
    13021 aggtagattt cgacaaattc tacaccgctg ttgaaatgac aaccaaggca aggaacatat
    13081 atatcatggg cttgagaagt tcttcgtttc tttctggata catggccttc tacatgaatt
    13141 tcatattcga taatgttaag ctggtacagc caggagcaaa tgacatcttc gagcagcttg
    13201 caaaggcatc agatgaggat gtcatcatat gtctcacata cccaagatat tccaaaagga
    13261 ccttggaggc ccttgaatat tcaaagaaca agggttgcag gatagtcagc ttcacagaca
    13321 gcctcatatc gccagcagct gacaagtctg acataaccat cattgccaga agtgacatgt
    13381 tgtcatttgt tgattcactt gtggcaccaa tgagcctgat caatgccttt ataatagctg
    13441 ttggaagtga gaagaaggag gacatcactc cctactttga agagcttgag aaggtttgga
    13501 aaaaatatag catctacgat gaaaacagca tgtaggacac aaaaaaataa atgcaagttc
    13561 aatccgttgt ggtaaaacgc aacggatttt tctatgtaat atataggtgg aggaaaacat
    13621 atcatagtaa ataatatcat aaatgataag aaaatgtatt ggatttgtgt tattattctt
    13681 atagagagca gattaaggag aggatttaat gaaattgaaa attggagtca taggtggtgg
    13741 accttctgga ataatggcag caggagcagc cgctgaaaag ggacatgacg ttgtcctgtt
    13801 tgaaaagaac aatcagctgg gaaagaaact attcataact ggcaagggaa gatgtaacat
    13861 tacaaatgca gcacccatcg atgaattttt tgacaatatt gtaacaaata aaaatttcct
    13921 atacagcagt ttgtatacct tcgataataa cgcgattatt gagctcttga gcaaatacgg
    13981 tttgaccaca aaggtggaaa gaggaaacag ggtattccct gaaagtgata aatccagcga
    14041 tgtgatcaag gccttcaaga ggtacctgga ccgtaacggc gttgatatta ggctaaactc
    14101 agatgtttcc cacattaaat acgatggtga gattttcacg gtcttcatca gggacaggga
    14161 ggaatataag tgtgacaagc ttattataac aaccgggggc aagagctatc cggccaccgg
    14221 gtcaacaggt gatggttatc tgttcgccaa gtcattgggc catactgtaa ctgatttaag
    14281 accaagtctc gttcccatcg agcttaatga tgaatgggtc aaggacctta tgggcttatc
    14341 cttaaggaat gttactttga cggcatacaa ggggaaaaag caagtacaca gtgagtttgg
    14401 agaaatggtt tttacccact atggaatatc aggaccaatt gtcctgtcca tgagtaacta
    14461 tgtaaatagc attaaatcga acatgagcct taccctggac cttaagcctg cactggatga
    14521 agagaaactg gatgcaagaa ttgtccggga ctttgccctg ttcagcaaca agcacttgaa
    14581 gaatgcatta aatgaactct tgcctcaaag gcttatacct tgggttatag aagcatctgg
    14641 aatatatcct gagaaggttg ttcaccagat tacaaaggag gaaagggaaa aacttgttag
    14701 ggcactgaag aattttccta tgcattttaa aggctttaga cccattgagg aagggattgt
    14761 gacttcaggt ggaataaatg taaaagagat aaatccatct acaatggagt ctaaaatcat
    14821 accgggatta tactttgcag gtgaaattat tgatatagat gcactcactg ggggatataa
    14881 tcttcaaata gcctattcaa ccggatattt ggctggaata aatgtataaa ttgcttgttt
    14941 aagttgcccg tttattatat aattgtatag gagagtttgt gggaatgtag tattatacga
    15001 gggctattac tgtagatatg aatccatttc tgtttgtgat ggatcaagag gaagattgaa
    15061 gacggaaacc atctaaaata gataaggtta tcagcatgat tttttctgct acaatacatc
    15121 cggacgagcc tacttttgaa attgatttcg gaagatgtca atagtcttgg gcctgataca
    15181 ctactgaatg caaaccacca atgggtggtt attctttttt tcggagggca gattatgagt
    15241 gaaaattgtt caattgcaat agatggacct gctggtgccg gcaaaagcac agtagccagg
    15301 attgtagcaa acaaattgag ttttgaatat attgatacag gagccatgta cagggcatat
    15361 accctgaagg tgctgcaaaa caatctcgat ccaaagaatc aggatgaggt aaattctctt
    15421 cttgattcca ctgatataag tttttccaat aatcatattt atcttgatga caagctggtg
    15481 gataatgaaa tcagggataa gagcattagt gataatgtct cctacattgc aagcagcaag
    15541 gatgtaagag agaggatggt ttcactacag caggaaatgg catcaagaaa gtcagtggta
    15601 atggatggcc gggacataac aactgtggtt ttgcctaatg cagactacaa atttttcgtc
    15661 acggcttcgg ttgaggaaag aggtagaagg agatatctgg aattggtaga caaaggcctt
    15721 caaaatatca ccttggatgg aatcattgat gatatcagaa ggcgtgatga gattgacagc
    15781 accagagaag agtcaccttt aaccaggaca gaggattcaa tccttgttga cacaaccgac
    15841 ctcagcattg aggaaactgt tgacctgatt atcaaccttg taaagggggg aaaataggat
    15901 tgttttattc attggtaaag gctatagcct ttgttgtctt caacattgta ttcagaatca
    15961 aggttgaagg taagcaaaac atcccaggtg acggcagaat tgtcctatgt tcaaatcaca
    16021 agagcaattg ggatccattg attctagctg ccatgtttcc aaggaagata tcctggatgg
    16081 gaaaaaagga actcttcgaa aacaaatttc ttaaggtagt cctcaattgg ttgggtgtat
    16141 ttcctgtaga tcgccaggtg gctgacttgt cagctgttaa gacagcctta agaattttaa
    16201 aatcagaaaa ggttctgggt attttccccg aaggaacgag ggttaccgag tacaatcctg
    16261 agaatgcaaa ggcaggtgtg gctttactgg caatgaaatc acaggcaccc gttttgccgg
    16321 tttatatcga aggtagttac aagctgttca gcaggcttga aattgtcatt ggggaaccta
    16381 tggaatatca taagaagtat acaggcaggt tgggcaacga agaatacagc aaaataagcc
    16441 aggaaatact ggcaaagata tacaagttgg gaggctaaga gttggagatt atattggctg
    16501 aaaacgcagg attctgtttc ggtgttgaca gagcagtcag gatgactgag gaagaattgt
    16561 caaagactga cagcagtgtt tactcctacg gtcccttgat tcacaatcca caggcagtga
    16621 aagcccttga agccaagggg ctggaaacaa ttgacagatt tgaggatata agtgagggaa
    16681 gaataattgt aaggtctcac ggagtccctg aggagacaat caatgatatc aaggagttgg
    16741 gattgcacct ggttgattgc acctgtccct atgtattggc tgtccacaaa aaagttaggg
    16801 actacagctc caaaggatac agcatagtta tcattggaga caaggaccat cctgaagtgg
    16861 ttgggataaa tggctggtgt gaaaaccagg cccatattgt caatagtgca gaagatgcaa
    16921 aggcattgcc agaccttgaa aagtcatgtg tggtttcaca gacaacgaac agattggata
    16981 agttccttga attgagtgga attgttgagg agaaatccca agagactgtt cttttcaaca
    17041 caatctgcaa tgctacaaag cttagacagg aggctgcaga ggaagttgca aaaaaggttg
    17101 atgcaatgat tgtaattggt ggaaaacaca gctccaatac aataaaactg gctgaaataa
    17161 gcagcagaca ctgcaaaaat gtgtttcaca tagaaacaat tgaagaattt gctttacaaa
    17221 ggctacaaaa ttttaataca ataggaataa ccgcaggtgc ttcaacacct gattggatta
    17281 ttaaggaggc tgttaaggta atggagaatt ataacaaaga tgaaatgatg gaagcaattg
    17341 agagttcctt taaaaggatc aacagaggtg acgttctaaa gggaacagtg ctttatgtaa
    17401 caaacaacga ggtaatggta aacatcaact acaagagtga tggaatcatc gacagaagcg
    17461 agatttcaaa agagcaggat gcagatccaa aagagctgtt caaagtaggg gatgaaatcg
    17521 acgtatatgt aatgaagctt gatgatggtg aaggtaatgt tgtgctttca gcaaaaagag
    17581 ttgattttat aaaagactgg gaaatcctgg aagagtcttt caagaacgaa gagattcttg
    17641 aagttaaggt tcttaatgca gtcaagggtg gacttacagt tcttgttagc ggtgtaaatg
    17701 gttttatgcc agcatcacag atttcaatga actatgtttc agacttgaac cagttcaagg
    17761 gacagacact taatgcaaaa attatagatt ttgatattca aaaaagaaga atgatccttt
    17821 caagaaaagc tgttgaaaag gttgaagtgg agaagaagag agctgaactt tgggagacca
    17881 tagaagaagg caaaatgctt actggagttg ttcaaagact tacagatttt ggtgcctttg
    17941 ttgaccttgg tggaattgac ggtcttattc acatatctga cctggcgtgg ttcagggtca
    18001 agcatccttc agaagtactg aaggaaggag acaacgtaga agttaaggtt cttgcctttg
    18061 acaaggagaa gaacagaatt tcacttggtc ttaagcaaac aactgaggag ccttgggaag
    18121 tattccttaa ggattacaag gttggagacg taatcgacgg ggaagttgtc aacatacttg
    18181 actttggtgc ctttgtaaga gttataaagg gcgtggatgg acttctgcat gtttcccaaa
    18241 tatccaagga ccatgttgag aagccttcag atgtattaaa ggttggcgac aaggtaacag
    18301 taaagattac ggaaatcaac gaagcagaca agaagatcag cctgagcgcc aaggatgcaa
    18361 tcgagacaga tgaaccagct gaagaggcgc ctgctgttga agaggaagct gaagagacct
    18421 atgagcaagc tgaatttaac acaagtgttg aagagcttac aaaagaagac taatatctaa
    18481 aaatacagaa tgcaaaatgg gttaaattta atttaaccca tttttttgaa gaaaataaga
    18541 gggggttaat agatggaaac caaggaacta ctggagatac tatccaacgg aaacggtgtc
    18601 tcaggctttg aatttaacct taaaaatgaa gtgatcaatg catttgacag gtatgcggat
    18661 tctgtccagg tggacaagct tggaaacatt attgcccata agaggggttc aggggatggt
    18721 agtgttaaaa tactcatggc tgcccacatg gatgagatag ggtttatggt aaaggatata
    18781 gacaaaaagg gttttataca tttcaccaat attggtggaa tagatccaag aaccatactt
    18841 gctcaggagg tggtcataca cggcagggag gaggtcccag gtgtaatagg gtccaagcca
    18901 ccccatctgc aggacgagtc tgaacaggaa aagtcaatca agatggaaga tatgataata
    18961 gatacgggat atgaaaagaa ggatttggac cagcttgttt caataggtga ttccattacc
    19021 gttggcagaa aaatggtctc attgttgaac agcagagttg caggcaaggc catggacgac
    19081 agggctggac ttgcagcact atttgaagcc atgaaggaac ttcaaaatat caaccattac
    19141 cctgatatct attttgttgc aacgatccag gaggaggtta caatggcagg agccttgact
    19201 gcaacatatg ggatcaatcc cgacattgga atagcggttg atgttggctt tggaagaact
    19261 cctgaactta acagttccaa ttcgattgag atgggcaagg gaccgggaat aacccttggt
    19321 ggcaacatac acccgggttt gagaaaaaga ctggtttcag tggcaaatga ctacaacata
    19381 ccaatacaga cagatgttag gccaggtccc actggaacag atgcaagggc catgcagata
    19441 acaagggaag ggatacctgc cttggtgctt tccattccac taaggtacat gcacacttct
    19501 gttgaggtac ttgaccttaa ggatgtttca cacacggcaa agctattggc atttttcagt
    19561 gcatccatca aagaaaatga aatggagggg ttattatgct tctaaaggaa ctaactgaag
    19621 cctccggcgt atctggaaat gagaaggaag tcagggacct tataaagtct accctggagg
    19681 gctatgtaga ccatatgaaa acagacggga ttggaaacct gattgtttat aagaagggat
    19741 ccaagcctgg tggaaggaag cttatgattg ccgcacacat ggatgaggtt ggactaattg
    19801 taaaggatgt agattcgtca ggcttgctca agttcatgcc agtagggggg attgacaagc
    19861 gagtgctggt atccaaggct gtgaaggttg gacaacaaaa acttccggga gtcatcggag
    19921 ccaagcccat tcaccttcaa aaaagaaatg agtggtcaaa ggttcttgat atggatgacc
    19981 tgtatataga cattggagcc agttccaagg aggaagcaga aaagcaggtt tccatagggg
    20041 actatgttgc ttttgacacc ccatacatgg agatgggaaa tggaaccata aaggccaagg
    20101 ccttggatga cagagttggg tgctcaatac tgatcgatct attgaaatcc gatattgatg
    20161 ctgaaatata tggtgcattt acagtcatgg aagaggttgg actagtgggt gcaggacctg
    20221 ctgcctatca gataaaacct gatatggcaa tagttctgga aggaaccctg tgctacgaaa
    20281 ttgaggacct ggactcccat gagatgccca caattatggg ccaaggccct gcaatttcac
    20341 tgattgacag aactactgta tatgatagaa gcttaagaga aaatgtggtg aggattgcag
    20401 aggagaatag tattccgtac caatacagga aaacctccat gggaggaaat gattcgggga
    20461 aaatacacat tacaggtaca ggttgtgcaa ccactggaat atcagttccc acaaggtata
    20521 ttcacagccc gttatctctg atcagcaaat cagactacca taatacgttt aaacttgtag
    20581 aggcaatagt taaagaattc gagaaggggg ctatataaat gaactttaat gtggaactac
    20641 tggagaagct agtgtccgta tatggacctt caggggacga atccctaata agcgctacaa
    20701 tccgtcagga aataggtggg tatgtggacg aggtcaccac tgatgcctta ggcaacctga
    20761 tagccaggaa aaaaggagac gggaagaaga taatggtctc agcccatatg gaccagattg
    20821 gattcattgt tacagacatc gacaaggaag gctttctaag atttggcaat attggaggaa
    20881 tatcccctga cataagcttg agccagaggg taatattcaa aaacggaaca gttggggcaa
    20941 ttttttctga acctttggac aagccatctg aaagaaagct tgaaaacatg tatatagaca
    21001 ttggagcaaa ggatagggaa gaagctgaaa aaatggtaag tgttggagat atgtgtatct
    21061 actacagccc cttttcccac aacgaaaatg tagtgttcac gccttacttg gatgacaggg
    21121 ttggttgttt tgtagctata gaggcactta aaagaattac tacaacagaa aacgacatat
    21181 actttgtatt ttcagtgcag gaagaggttg gtttgagagg agccaaaaca gctgcctatg
    21241 gaatagagcc tgattttggg attgcggtgg atgttaccgc acatggagac actcccaagg
    21301 caaagagatt cgcaattggt cttcacaaag gtgcagccat aaaggttaag gataactcaa
    21361 tcataaccca tccttcagtt aggaattcac tgattgacat tgccaaggca aagggcatag
    21421 cctaccagct ggaggtcctt gagtttggag gaacagactc aggtgcaatt cacgtgacca
    21481 aaaccggagt accctcgggg gtaatctcaa ttccaaccag gtatgtccat tcaaccgttg
    21541 aaatggcttc aaaggaagat attgaaagca gcatttcatt gttggaagga tatctctcaa
    21601 agaaactcta gcaggaaaaa agaaaagtct gttctggaag tgggtgcata caccttcatc
    21661 cagcagactt tttattgcga aaatgtgtta taatttaagt tgtagcaatt agaagcaagc
    21721 aaggagaaat gataatgaag aaatatctta taaatacatc cggctgtcag gcaaatgagc
    21781 tggattcaga gaaaatatcc tgggtacttg atacgatggg gtatgaaccg acaaatgata
    21841 aggaagaagc ggatataatc cttttcaata cctgtgccgt cagaaagagt gcagaggaca
    21901 aggtgtttgg acagcttgga gagctgaagg agcttaagag gagaaagccg gaagtgatgc
    21961 ttgggatctg cggctgcatg atgcaaaggg aggatataag ggacttcttc ttatccaaac
    22021 acaggcatat tgacctagtg tttggaacaa acaatatcca taaactaccc cagttgattt
    22081 ctagacagct tgagacagga aaaacaatag tggacattat tgaggacacc agagaaatag
    22141 atgaaaatat cgatgcgcac aggaaatatt catacaaggc ctttgtggat atcatgtatg
    22201 gttgtgacaa ttactgcacc tattgcatag ttccctacac aaggggaagg gaaagaagca
    22261 gggaaccgga atccatcctc caggaaataa gaaagctagt aaggtctgga gtcaaggaag
    22321 tgacattgct gggtcagaac gtaaactcct atggaaagac actgtccatg gattacacat
    22381 tccctgacct gctgagggat gtcaacgaca tagaaggaat tgaacgaatt cgatttatga
    22441 catcacaccc caaggacttc acagaaggcc ttataaaggc ctatgttgaa ctggacaagc
    22501 taagccccca cctccacctt ccggtgcagt cagggagcga caggatgctt aagatgatga
    22561 atagaaagta taccaggggt gaatacctgg aaaaggtaaa agccattaag gaaagagttc
    22621 ccaatcttgc aataacgaca gatattattg taggttttcc tggagagaca gaggaagatt
    22681 ttcaggaaac aatcgatttg gtgaaagagg ttgagttcga ttcagcctat acattcctat
    22741 attcaatcag ggaaggaacc atagccgcca agattgaaga ccaggtgccc gacaagataa
    22801 agcatgtaag gtttcagagg cttttggaca cccttaatcc aataggtctt aagctaaacc
    22861 agaagctatt gggtacggtc caaagggtat tggtggaaga ggtaagcaag aatgacagca
    22921 aggtaatgag tggcaggact gaaagtgcca agctggtcca cttccctgga ggggatgagc
    22981 tgattgggaa aattgtaaat gtaaggatcg aaaatgttaa aacatttact ctagaaggag
    23041 aaatgatcga ctaggaggac caaatgggaa cacttactcc aatgatgcaa cagtatgtgg
    23101 atacaaagaa aaaataccag gacgcactgc tattcttcag actgggggac ttttatgaga
    23161 tgttttttga ggatgcgaaa atagcctcca gagagcttga gattgccctg accagcaggg
    23221 actgtggact tgacgaaaag gcacccatgt gtggagtacc ccatcatgtg gcagaaacct
    23281 acgtttccag acttgttgat aagggctaca aggtggccat ttgtgagcag gtggaggacc
    23341 cggccctggc caagggattg gtcaagagag atgtggttag gattgtaact ccaggaacca
    23401 tcatggatgg gaatctcctt aatgaaaagt caaataactt tctggccagc atcttcctgg
    23461 atgaatatgg agtcggtctt tcctatgttg ataattcaac aggagaaatg ttcaccactg
    23521 aatttattgg tgaagaggag aacagtcaaa ggtttgtact tgatgaactg ggaaaggtaa
    23581 tgccctctga aattgtctgc aacaagggct ttgcaaagga taagaagctg gttgcagaca
    23641 taaggaacaa aataaatcct tacattaatg tgcaggatga tgcatccatc gatgtggaaa
    23701 ccagcaggga gtccattctc ggatttttaa gtgaggaaga ttatgaaaag aacagcctga
    23761 aggaaaagat atactccata ctttcattgg gcaggcttgt tgattatctc agggataccc
    23821 aattcaatgc aatcgatcat attaaaaacc tgcagtatta ccagccagac cattacatgt
    23881 tgatggatat caatacaagg gctaatcttg aaatccacga aaccatgaga tccaaggaaa
    23941 aaaggggggc cctgattggc attctggaca aaacgaagac cgcaatggga ggcaggctat
    24001 tgcggaagtg gcttgagcag ccattgcttg ataagaagca gatagagtcc aggctggata
    24061 tggtggagta tctttgtaac aatccatttc agcttgagga aatcaaggat atactaaaga
    24121 caatatatga cattgaaagg cttataagca aaatatcaaa cggtaacccg ggtggtagag
    24181 atatgctggc cttaagtcaa tccatacaga acattccaca gctaaagggt attctttccg
    24241 aaaccggatt agcccaattc catcgtctac aggaagaaat ggacgattta atggatattc
    24301 atcaagtgtt gaccagtgct attgacgaga atccacccct ttcagtcaag gaaggtggta
    24361 tcataagaca agggtacaat gaacatctgg atgacttgag agaaagcaag accaggggaa
    24421 aggaatggct tgcaaggttt gagtctgatg aaaaaacaag aacgggaata aagaccctta
    24481 aggtaggcta taacagggtt atgggctatt atatagacat caggaaatca agcatcgaca
    24541 gtgttccgga aaactatata agaaagcaga ccctggccaa ttccgaaaga tactatacaa
    24601 ctgagcttaa ggagattgag gacaagatac ttggagcgga tgaaaaggca attcaactgg
    24661 agtacgagct ttttcaggag attaggaatc agatcaagga agaaattaaa cgtcttcaaa
    24721 aaaccagtaa gcacattgca gtactggatg tggtcatgag cctggcaatt gttgcaaatg
    24781 ccaatggttt tacccgacca agccttaata caaagggaat aatcaacata gaagagggaa
    24841 gacacccagt ggttgaagcc accatcaaac accagcta
//
LOCUS       DASRQF010000146         2542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_459127, whole genome shotgun sequence.
ACCESSION   DASRQF010000146 DASRQF010000000
VERSION     DASRQF010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459127"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VFD79_03915"
     CDS             complement(<1..432)
                     /locus_tag="VFD79_03915"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HZH93225.1"
                     /translation="MKGMVYMRSDSVKTGTQQAPHRSLFNALGMTEEEMKKPLVGIVS
                     SYNEIVPGHMNLDKIVDAVKLGVAAAGGTPMVFPAIAVCDGIAMGHTGMKYSLVTRDL
                     IADSTEAMAMAHGFDALVMVPNCDKNVPGLLMAAARINVPTI"
     gene            complement(616..1053)
                     /locus_tag="VFD79_03920"
     CDS             complement(616..1053)
                     /locus_tag="VFD79_03920"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZH93226.1"
                     /translation="MNSELKNLDLIDLISERHLQLRDIAEEDWNKASDIHISNSEWFI
                     LARIYQRNPSVSQVTRRVDMSRQAVHKFAKGLQAKGLVEIRDSRHNKRDKCLRLTSLG
                     KECYEKNLILKKDLESKIGELIGQDRLDSLKEILALNWGIKEY"
     gene            1236..2255
                     /gene="recA"
                     /locus_tag="VFD79_03925"
     CDS             1236..2255
                     /gene="recA"
                     /locus_tag="VFD79_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HZH93227.1"
                     /translation="MKVDEKKLAALDDAIRQIERQFGKGSIMKLGSDEVVNIEVIPTG
                     SLALDVALGVGGFPKEKVIEIYGPESSGKTTLALHTIVQSQMRGGTAAFIDAEHSLDP
                     SYAKKLGVNTDELIISQPDSGEQALEITEALVRSGAVDVVVVDSVAALVPRAELEGEM
                     GASHVGLQARLMSQALRKLAGAISKSRCVVIFINQLREKIGVMFGSPETTSGGRALKF
                     YASVRLDIRRIDSIKSGDSIVGNRTRVKVKKNKVAPPFKQAEFDIMYNEGISREGDVL
                     DLGVEEDFVKKSGSWFSYMDEKLGQGRENSKQFLRDNPAILDEIETKIREKHSLTIEV
                     KSEGK"
     gene            2256..>2542
                     /locus_tag="VFD79_03930"
     CDS             2256..>2542
                     /locus_tag="VFD79_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93228.1"
                     /translation="MKLINKNVEHVSFGKGKITKADENHISVKFIGEEEEKLFQYPQA
                     FGSFLKIMDSKIQKSIMKDYDASVEQNNLQLEKIEQARIDAKEKVKDEAIEA"
BASE COUNT          793 a          470 c          585 g          694 t
ORIGIN      
        1 aatagtaggt acatttattc tggcggcagc catcaacaat cccggaacgt tcttgtcaca
       61 attgggaacc attacaagag catcaaagcc gtgtgccatg gccattgcct cagttgaatc
      121 tgcgataagg tctcttgtaa caagggaata cttcatgcct gtatgtccca tagctattcc
      181 gtcacaaact gcaattgcag gaaataccat aggggtacct ccggcggctg caacacccag
      241 ttttacggcg tccacaattt tatccaggtt catatgacct ggaacaatct cattgtagga
      301 gctgacaatt cccacaaggg gctttttcat ttcttcctcg gtcattccaa gggcgttgaa
      361 cagggatctg tgaggagcct gttgtgtacc tgttttaact gaatcgcttc tcatataaac
      421 cattcctttc atttagtgct tttgcaagca gtataataat ctgaaaatat acatttttac
      481 aacaattcca taacatcagt atattactat ttcgttataa taaattactt agtatctcaa
      541 aaaaaatatg gatataattt tgttggaaaa ttaatatcca tataaatata ttacgataag
      601 gtgaagttga aattgtcaat actccttgat accccagttc agtgcaagaa tttccttgag
      661 tgaatccagc ctatcctgac caatcaattc accaatcttc gattccaggt ccttcttcag
      721 aatcaggttc ttttcgtaac actccttgcc cagactagtc aaccttagac atttgtccct
      781 cttattatgc cttgaatccc taatctccac taggcccttt gcctggagtc cctttgcaaa
      841 cttatgaact gcctgtcttg acatgtcaac acgtcgtgta acctgtgaga cggaaggatt
      901 tctttgatat atcctggcaa ggataaacca ttctgaattt gatatatgaa tatcgctggc
      961 cttattccaa tcttcctcgg caatatccct aagctgtaga tgtctttcgc tgataaggtc
     1021 tattaaatct agatttttca attctgagtt caagattcca cctcctgaga attttcaata
     1081 ataagtatat aggtggcatg gcaaattgtc aaccaagttg acaaaaatgg attaaaggga
     1141 taaaattaga tagtcaacaa aattgacaag caagaataca agcctgtaat ttacttgatt
     1201 tttgaaatat attaagggaa gggaagttga taaggatgaa ggtagatgaa aaaaagcttg
     1261 ctgcactgga tgatgcaatc agacagattg agaggcagtt tggcaagggt tcaatcatga
     1321 agctgggtag tgatgaggtg gttaatattg aagtgatccc cacaggttcc ctggctctgg
     1381 atgtggcttt gggggttggg ggatttccca aggaaaaggt aattgaaatc tatggtcctg
     1441 aatcatccgg aaaaacaaca cttgccctac atacaatagt gcaatcccag atgaggggtg
     1501 gaacagctgc ctttatagat gcggagcatt ccttggaccc atcatatgcc aagaagcttg
     1561 gtgtaaatac agatgagctc ataatatcac agccagattc cggagagcag gccttggaga
     1621 ttacagaggc cctagtcagg tctggagcgg ttgatgtagt tgtagtggat tcagtggctg
     1681 ctcttgtccc cagggcagaa cttgagggtg agatgggagc atcacatgtt ggtttgcagg
     1741 caagactaat gtcccaggcc cttaggaagc tggctggtgc aattagcaag tcaagatgcg
     1801 tggtgatatt cataaatcag ttgagggaga aaataggtgt tatgtttggg agtcctgaaa
     1861 ccacatcagg aggacgggca ttgaagttct acgcttccgt caggctggat ataagaagga
     1921 tagactccat caagtcaggt gatagtatag tgggaaatcg aaccagggta aaggtaaaga
     1981 agaacaaggt ggcaccgcca tttaagcagg cggaatttga cataatgtat aatgagggga
     2041 tatcaaggga gggagacgta cttgaccttg gagtagaaga ggattttgtc aagaagtcgg
     2101 ggtcctggtt ctcatacatg gatgaaaagc ttggccaggg aagagagaac tccaagcagt
     2161 ttcttaggga caatccagca atccttgatg aaatagagac aaaaatacgg gaaaagcaca
     2221 gtttaaccat tgaagtaaaa tcggagggga agtaaatgaa gcttattaac aaaaatgtag
     2281 aacatgtcag ctttggtaag gggaaaatca ccaaggctga tgaaaaccat atttcagtta
     2341 agtttattgg agaagaggaa gaaaagctat ttcagtatcc gcaggccttt ggaagttttc
     2401 tgaagataat ggactcaaag attcagaaat caattatgaa ggactacgat gcttcagttg
     2461 aacagaataa tcttcaactg gaaaaaattg agcaggcaag aattgatgca aaggaaaaag
     2521 tcaaggatga agcaattgag gc
//
LOCUS       DASRQF010000147        26033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_427602, whole genome shotgun sequence.
ACCESSION   DASRQF010000147 DASRQF010000000
VERSION     DASRQF010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 26033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26033
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427602"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /gene="tsf"
                     /locus_tag="VFD79_03935"
     CDS             complement(<1..645)
                     /gene="tsf"
                     /locus_tag="VFD79_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HZH93229.1"
                     /translation="MAISASLVKELREKTAAGMLDCKKALEKTDGDIDKAIDYLREKG
                     LASASKKSGRVASEGLVEAYIHGGRIGVLIEVNSETDFVAKNDEFKEFVKDMAMQVAA
                     ANPKVVSREELSEEEIAHEREIQLNKVVNEGKPAHVAEKIVEGRMEKFFEQICLLDQG
                     FIKDPSVKVKDLLAAKISKIGENIVIRRFTRYEVGEGLEKKEENFAEEVAKQMNM"
     gene            complement(711..1436)
                     /gene="rpsB"
                     /locus_tag="VFD79_03940"
     CDS             complement(711..1436)
                     /gene="rpsB"
                     /locus_tag="VFD79_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HZH93230.1"
                     /translation="MSVVSMKSLLEAGVHFGHQTRRWNPKMAQYIFTERNGIYIIDLQ
                     KTVKKIDDAYNVMKEVVENGGEALFVGTKKQAQEAIENEAKRCGMHFVNQRWLGGMLT
                     NYKTIRKRIDRLHQLEKMEEDGTFEVLPKKEVIKLRHEAERLEKFLGGIKTMTRVPDV
                     LFVVDPRKEKIAVQEAHILGIPVIGIVDTNCDPDEIDYIIPGNDDAIRAVKLLTETMA
                     NAVIEAKQGEQNESEVNVEATEE"
     gene            complement(1630..2403)
                     /gene="codY"
                     /locus_tag="VFD79_03945"
     CDS             complement(1630..2403)
                     /gene="codY"
                     /locus_tag="VFD79_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HZH93231.1"
                     /translation="MESLLKKTRRLNKTLQSYGSQPVSFTDLSKILSDILEANVYIAS
                     RKGRVLGYALSDGFECDVINSEVVKERRFPKKYNEELLKVIETSENIKEVTDCVFDHV
                     TECGYPDKISTVVPINSGGNRLGTLVVARFGKEFTVEDLILCEYSATVVGMEILRATT
                     EEIEEDARKKSIVQMAIGTLSYSEKEAMEHIFHELDGMEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIKILNEKLLDELKRK"
     gene            complement(2512..4590)
                     /gene="topA"
                     /locus_tag="VFD79_03950"
     CDS             complement(2512..4590)
                     /gene="topA"
                     /locus_tag="VFD79_03950"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HZH93232.1"
                     /translation="MSKYLVIVESPAKAKTISKILGRNYKVVASVGHVRDLPKSRLGV
                     EIENDFEPDYINIRGKGPLIKDLKKEAKKADKVFLATDPDREGEAISWHLAYILGLDE
                     NEKYRVEFNEITKERIKQAIKSPRNLDKNLVDAQQARRILDRLVGYKISPLLWKKIKK
                     GLSAGRVQSVAVKLICDRESEIDEFIPVEYWTIKALLNKDEQGFESNFYGVEKNGKDE
                     KLHINSKSESDSILKELKKNEFIVNEVKKGKRKRNPYPPYTTSTMQQDASKKLGFSTK
                     KTMMVAQQLYEGIDLKKGGITGLITYMRTDSTRVSNEAMSVARSYIENKFGKEYSNGG
                     RSYSNKSKKETQDAHEGIRPTYIDHSPDDIKDYLNKDQYRLYKMIWSRFMGSQMKEAL
                     YNTLNVRIMNGQYVFRSNGSQLVFPGFLTIYSTTDEETSDMALPTMNKGDILDLDKLE
                     PKQNFTQPPARFTEASLIKLMEEMGIGRPSTYSPTISTILSRGYVVLEKKSFYPTELG
                     LLVNELLTEYFTHVVNEKFTANLEDELDDIASGSTFWKEVVRDFYKDFHKELVIAEEE
                     IDKIEIEDEVSDVICEKCGRNMVVKHGRFGKFLACPGYPECKNTKPIVDKIEVPCPKC
                     GGDVVRKRSKKGRVFYGCNNYPDCDFVSWDEPVKEKCPDCNGPMVIKRLKSRTTVKCV
                     DSNCGYSKNI"
     gene            complement(4619..5710)
                     /gene="dprA"
                     /locus_tag="VFD79_03955"
     CDS             complement(4619..5710)
                     /gene="dprA"
                     /locus_tag="VFD79_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HZH93233.1"
                     /translation="MNLERDTLIWLNSLGIYSDVIDKLRSYLGDISEIHDFNHSDLEN
                     LGLLGSGILKRIAKSDFKSSIEEYKEKLDGLGIDVVTVCDNTYPEELSEIPDRPLVLY
                     IKGKLQPRDHIAMGIVGSRKATNYGKWAAERFARDLTNMGVTIVSGMAAGIDSIAHRT
                     ALENGGRTIGILGNGLDNVYPKSNFRLYKDAADNGALISEFPPGAQPYNFHFPMRNRI
                     ISGLSLGIIVVEAQQKSGSLITAHHALAQGKDVFALPGNINSIYSQGTNLLIKDGAKP
                     LLDMDDILEEVRELQLIKKTKDEDRLNEIDLSETEMRVLEFLKEGPAHSDIIVLKSGL
                     DIQTVISTLTILELKGIIKEMSSRIFTIV"
     gene            complement(5714..7240)
                     /locus_tag="VFD79_03960"
     CDS             complement(5714..7240)
                     /locus_tag="VFD79_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HZH93234.1"
                     /translation="MYSKVKTCVLQGLDGHIVEVEVDLSRGMPMFTIVGLPDAAIKES
                     KERVRTAIRNSGFEFPLSRITVNLAPANLKKDGSQMDLAIAAGILISDGLVEGFDSEE
                     VAFLGELSLDGTVNPIDGALAMIISLRNLGIRRCIIPYGNREECGIIEDVDIIPVENI
                     FDLINHLNGENIIRPYIAEIKAEDTSGDSQYDFSDIKGQDGLKRAMEVSAAGGHNLLI
                     IGPPGSGKTMAARRIPGILPHLSFEESIEVTKIYSISGLLRKSRLMKERPFRAPHHSS
                     SGVSIIGGGRIPKPGEVSLAHNGVLFFDELPEFQKSVLEVLRQPLEDGFVHISRANAS
                     LTYPSDFMFVASMNPCPCGYYGDPMHQCTCNQNQIDRYLGKISHPLLDRIDLHIEVLP
                     VKYEELNTDIQSRSSDDMRKSVSRARQTQIDRFSELNIYTNSQMGNKEIKRFCKLTPS
                     SEKIMKSAFDKLRFSGRTYNKILKVARTLADLDGVENIDDKHLLEAIQYRSLDKKYWG
                     "
     gene            complement(7312..7668)
                     /locus_tag="VFD79_03965"
     CDS             complement(7312..7668)
                     /locus_tag="VFD79_03965"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HZH93235.1"
                     /translation="MKSHIKTGRIGEQLALNYLTDKGYSVLETNYRNKIGEVDIIAYD
                     KDILAFIEVKTRMGNDYGYAYESVNSRKQKKIANACLMYLQKNKMYDVQVRFDVIEVY
                     PMEKKIINHFENAFSL"
     gene            complement(7671..8282)
                     /locus_tag="VFD79_03970"
     CDS             complement(7671..8282)
                     /locus_tag="VFD79_03970"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004822824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HZH93236.1"
                     /translation="MFEIEQELYFEGHKHIACIDEVGRGCLYGDVVAGAVILPAGLTI
                     DGVKDSKKLSEKKREKLFDIIRDEAVAYGFGVVSPEIIDKINIKQATRLAMKLALQNL
                     RDKEGNRISPSYILVDAEEVESNIPQKAVIDGDNLVHGISAASILAKVYRDRMCQEWD
                     MVHPGYDLAKNKGYGTKNHRDALVELGPTNKHRMTFLKNILKG"
     gene            complement(8284..9141)
                     /gene="ylqF"
                     /locus_tag="VFD79_03975"
     CDS             complement(8284..9141)
                     /gene="ylqF"
                     /locus_tag="VFD79_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HZH93237.1"
                     /translation="MNINWYPGHMKKTRESIQKNLKMIDIVFELLDARIPYSSQNPVI
                     ESLVGGKPRIILLNKSDLADANANRQWQDHYKNLGISSILLNSLSGKGVDDLVTEARR
                     VMDEKRVNNARKGIINQPIRAMILGIPNVGKSTLINSLAGRRSAKTGNRPGITKTNQW
                     IKTKGGIELLDTPGILWPKFEDEQVGINLAFTGAIKDEILDTETLALKLVEKLMIVKS
                     QALIDRYNIEMSKDCEPLEAMEAIAAKRGCLLKGGTFDYEKVSRIILDEFRRGMMGKI
                     TLEYPKRGE"
     gene            complement(9191..9538)
                     /gene="rplS"
                     /locus_tag="VFD79_03980"
     CDS             complement(9191..9538)
                     /gene="rplS"
                     /locus_tag="VFD79_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005956838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HZH93238.1"
                     /translation="MDIIKMLEDEQLRNDLPEFNVGDTVQVHYRVIEGTRERVQIFEG
                     TVLKVQGGGARETFTVRRLSYGVGVERTFPIHSPRIEKLVVTRKGKVRRAKLFYLRER
                     QGKSAKVKEKRFY"
     gene            complement(9639..10373)
                     /gene="trmD"
                     /locus_tag="VFD79_03985"
     CDS             complement(9639..10373)
                     /gene="trmD"
                     /locus_tag="VFD79_03985"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HZH93239.1"
                     /translation="MKIDVLTLFPEIFDMLKDYSIVGRAIASEAIDVNAINIRDYSTD
                     KHKRVDDYPYGGGRGMVMKPEPIHSAIESCRRVGSRVIYLSPQGKILNQSLVKELANE
                     DHLILLAGHYEGVDNRIIENYVDDEISIGDYVLTGGELPAMVLIDSISRLMPGVLSSE
                     ESYEIESHYEGLLEYPQYTRPRVFKGFKVPDVLLSGDHSKIDIWRRVSALRTTFMKRP
                     DMLEEIENPKDQLDLMDSLLKMIGKR"
     gene            complement(10370..10870)
                     /gene="rimM"
                     /locus_tag="VFD79_03990"
     CDS             complement(10370..10870)
                     /gene="rimM"
                     /locus_tag="VFD79_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587774.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HZH93240.1"
                     /translation="MEYTVIGKLTGTHGIKGDLKVFPLTDSTERFFELEKAYVGENKI
                     KVHVSGVKLHKGMVLLRFKGLEDINKVLDFKDEYLYVDEEDKVRLPENHYFISDLVGC
                     RVYDKSETFIGELTDVIQNSANDVYVVTSDTGKEYLIPAVEAFIASVDVVEKIIIVDP
                     IEGMIE"
     gene            complement(10894..11124)
                     /locus_tag="VFD79_03995"
     CDS             complement(10894..11124)
                     /locus_tag="VFD79_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HZH93241.1"
                     /translation="MGELVEFIAKALVDNPDQVSVNEVEGSQSIIIELKVAPDDMGKI
                     IGKQGRIAKAIRTVVKAAAIKDNKRVVVEIIQ"
     gene            complement(11142..11426)
                     /gene="rpsP"
                     /locus_tag="VFD79_04000"
     CDS             complement(11142..11426)
                     /gene="rpsP"
                     /locus_tag="VFD79_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HZH93242.1"
                     /translation="MAVKIRLRRMGAKKTPFYRIVVADSRAPRDGRFIEELGYYNPLT
                     EPKTVKIDNEKAAKWLKNGAKPTETVERLFKNSGLYDKAEETTTEEETAE"
     gene            complement(11460..12800)
                     /gene="ffh"
                     /locus_tag="VFD79_04005"
     CDS             complement(11460..12800)
                     /gene="ffh"
                     /locus_tag="VFD79_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HZH93243.1"
                     /translation="MVFESLSEKLQNALGKLKGKGKISEKDLDAALREVRLALLEADV
                     NFKVVKDFIKKIKERALGAEVLESLTPGQQVIKIVNDELTALMGDREVKLNMASTPPT
                     VILMCGLQGAGKTTTSGKLANNLKKQNKRPLLVACDIYRPAAIKQLEVVGEKVGVPVF
                     SMGDKHNPVDIAKAAMEHGKKNGNDVIIIDTAGRLHIDENLMQEITEIKEATDPDDIL
                     LVLDSMTGQDAVNVAQAFNDQLSITGVVLTKLDGDARGGAALSIRAVTDKPIKFVGMG
                     EKLDQLEPFHPDRMSSRILGMGDVLSLIEKAQSNIDEKKAMELEKKMRTAQFTFDDFL
                     DQLEQMKNMGPLDEILGMIPGMNSKALKGLNVDNSELARIEAIIKSMTKKERDNPDMI
                     DSSRRKRIAAGSGTNVQDVNKLLKQFKETKKMMKKFSDMSKPSKKKGKMGFPFF"
     gene            complement(12818..13177)
                     /locus_tag="VFD79_04010"
     CDS             complement(12818..13177)
                     /locus_tag="VFD79_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxM family DNA-binding protein"
                     /protein_id="HZH93244.1"
                     /translation="MEKLVRIGILFDFYGKLLSPKQYQVIDLYYSNDLSLSEIGEVLQ
                     ISRQGVFDTLKRAEDKLLKYEEKLGLVDKFYESQEAIRESRNLAREICIIGEKSSDDE
                     IVEKAKMIESLISDILL"
     gene            complement(13257..14375)
                     /gene="ftsY"
                     /locus_tag="VFD79_04015"
     CDS             complement(13257..14375)
                     /gene="ftsY"
                     /locus_tag="VFD79_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HZH93245.1"
                     /translation="MFNWFKKDKRKSEEYNQTLESQNTDNGLEIEDEDDDLEDGNEEK
                     TSSMVEDALGLDDEAEVDDAGPVSGGILDRLKNGLAKTRKDMSNKIEDIISGYKGVDE
                     ELFDDLEDVLVSADVGVNPTMMIIDRLRERVREEKVSDPKDVKGLLKEEIKKLMIESV
                     PGNDLDLLPHPSVLLVVGVNGVGKTTTIGKLAYGFKQNGKKVLIAAGDTFRAAAIEQL
                     EEWSKRAGAEIISHTEGSDPAAVIFDGIQAAKARKADILICDTAGRLHNKSNLMNELN
                     KIFRIVEREYGEATKEVLLVLDATTGQNAINQAKVFKEVANITGVALTKLDGTAKGGV
                     AIALQTELKLPIKIIGVGEKVEDLQPFDTESFIDAIFE"
     gene            complement(14386..17961)
                     /gene="smc"
                     /locus_tag="VFD79_04020"
     CDS             complement(14386..17961)
                     /gene="smc"
                     /locus_tag="VFD79_04020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HZH93246.1"
                     /translation="MYLKKVEIQGFKSFAEKTEVVFNDRVTAIVGPNGSGKSNIADAI
                     RWVLGEQSVKNLRGTRMEDIIFSGTEKRKALGYSEVTIVFDNTTGRIPIDYREVAVTR
                     RMFRSGESEYYINKNSCRLKDIRELFMDTGVGKEGYSIIGQGKIDEILSNKPEDRRNI
                     FEEAAGIVKYKSKKQEALRKLERTQTNLTRIGDLLHEIKNQKENLQTEAEKAKEFQKL
                     YGQLRRLDSSLSVYEVNRLKTKLDDAEGKRIDLDSEKAIIEADSTQVEEGYSTLKSSI
                     ESLERIIEEKRTLKMESIQEFESSKNRVKILEERESHLKKEVARLNEEIAKGKDFLHA
                     GDTRKDTSTIEIDRLDNEVAMLKAHLDGKFEERKALDGRTLSLEKTIADKKNQLMTLY
                     SSLSDKKSESNTIASFEDSIKGRLDILTEDENSIADKCSRLKNGLENKSRDLESLLEA
                     KRINEEKIMEVEAKASKLKARIGSEQAKLQEDQKELDASISSQKLYRNMEKEYEGYYN
                     SVKSLVKAINKDTKLGDGFIGTVADLFTVDKKYMKAIEVALGSSIQNIVVKDDVSARR
                     MIDYLKSEKKGRATFLPLNTVSGWEISIEDRDRKEYGLIGAASSILEADQKYSKIFMN
                     LLGRTILANSLEDAINYSKKSGFKSRIVTLEGEIINPGGSLTGGSTKNQSISILNRKH
                     TLADLEIKIKNIKLKIEAKTKAIVTIESELNQLGKAYDNLIQEKTGLIDKTTVLEKEM
                     DNMEYQYEIQNESINSKRTEISGLRDELKELETKKAELKSDQDALKDSHDKLYTDLAG
                     MQEALDEYKNNRTILDNDITETRIKLNALEKSLGTFKERIQENELSISETRESIKLKE
                     TELDRLEMEISENTLEKKSLCEYIQRQSTTEEEESLLLKEKVGEKEKLMKEFYQIQDR
                     LRELTKRQTSVERELNKLEVDQAKAQLQIENILEKLENDYEITLAEAEKIYSDIEDIR
                     GTKKTISELKQGIKELGNVNISSIEEYDNVLERYEFLNKQVVDLNGAKEDIESVVKDM
                     EKKMRVQFRKSFEEINERFSETFSVLFKGGKARLEIEDGDDILTAGIDIMAQPPGKKL
                     QSLTLLSGGEKSLTAVALLFAILKTRPSPFCILDEIDAALDEANISRYTSYLNSLEDN
                     TQFVLITHRKTTMEIADVLYGVTMEEEGVSRMISLKLKDYDEIAS"
     gene            complement(18023..19105)
                     /locus_tag="VFD79_04025"
     CDS             complement(18023..19105)
                     /locus_tag="VFD79_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZH93247.1"
                     /translation="MTRHYIIPIFVPHFGCPHDCVFCNQRKITGLSTDVTVEDVKGII
                     DDHLETFKRDSFIEVAFYGGSFTAIQMEIQRELLRVPYEYKMEGKIHNIRLSTRPDAI
                     NEDILGLLKDFQVDTIELGVQSLDHEVLAMSERGHRDEDVYASSGLIREWGFKLGHQI
                     MLGLPGDSQAKSLETAKKVLEMGPDMIRIYPTLVIRNTPLERQMKDGLYAPLSIEEAI
                     ETAAIILMAFTAHDIPVIRIGLQPTENIQMGRDVVAGPFHPSFRQLVEEQVFSLLLRE
                     YIARNKIETKGKSLTIKCANSRTSIIAGQHGKNKERLIEDYGLTKVRIIPFRGKDSRI
                     ILSMENDERVIDLGEETKLYINNNPL"
     gene            complement(19098..19817)
                     /gene="rnc"
                     /locus_tag="VFD79_04030"
     CDS             complement(19098..19817)
                     /gene="rnc"
                     /locus_tag="VFD79_04030"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HZH93248.1"
                     /translation="MSGYLDREKLEEIQNNLNYRFEDVELLETALTHSSYANEHGMHA
                     SKSNQRMEFLGDTVLGLVVSEYLFMKYPFYPEGELTKIRAKVVSEPSLALAARRIDLG
                     AFLLLGKGEESTGGRERDSILADASEAVTGAIFMDSNYETVREFLLKNFETTIVKAVA
                     KGSLFVDYKTGLQEKLQKITREKLVYSVVKEEGPDHNKLFHVNVAVGGDVIGSGIGRN
                     KKEAEQMAAKEALILMGEDYD"
     gene            complement(19834..20064)
                     /gene="acpP"
                     /locus_tag="VFD79_04035"
     CDS             complement(19834..20064)
                     /gene="acpP"
                     /locus_tag="VFD79_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZH93249.1"
                     /translation="MITEKIISIITTQFNVPEKDVTMETSFRDDLNADSLDLVELVMA
                     LEDEFGLEVEDDDVELIKTVGDARNYIKGKLE"
     gene            complement(20077..21060)
                     /gene="plsX"
                     /locus_tag="VFD79_04040"
     CDS             complement(20077..21060)
                     /gene="plsX"
                     /locus_tag="VFD79_04040"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HZH93250.1"
                     /translation="MRIIVDGMGGDNAPEAIVKGAVEAVNEYGIDILITGNESLIKEH
                     LENHSYPAEKIQIMDAPEVITNDDNPTLAIRRKKNSSMVKGMQALADGEGDGFISAGN
                     TGALLAGGLFIVKRIQGIDRAALTVLYPTLSGASLLVDAGANMDTKPEYLEQFALMGS
                     IYMEEVMGVKEPRVGLINVGTEDTKGNQLAKDTFVALKDSNMNFVGNIEARDLPFGVA
                     DVMVCDGFVGNVVLKLSEGMASSIFTLMKESFVADTRSKLGALMLKPQLKNMKKRLDY
                     REYGGAPLLGTVKPIVKAHGSSDFYAFKNGIGQLKTFIEKDVIGRIENKIL"
     gene            complement(21177..21356)
                     /gene="rpmF"
                     /locus_tag="VFD79_04045"
     CDS             complement(21177..21356)
                     /gene="rpmF"
                     /locus_tag="VFD79_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004822430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HZH93251.1"
                     /translation="MAVPKRKTSKARRDKRRASSYRLNKTTVVECPQCHEPKQPHRVC
                     RSCGYYKSREVIVVE"
     gene            complement(21370..21885)
                     /locus_tag="VFD79_04050"
     CDS             complement(21370..21885)
                     /locus_tag="VFD79_04050"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HZH93252.1"
                     /translation="MKIDLSDFLDSGKQVMSFSFDLSRDEFPIEDVSSIVDPIHIEGN
                     FYKVDGEILMESKGNYLYQAPCDRCLAESSNEISFKASGKLMEEESGNKIEDEESDEL
                     VYYKDKYVNLNDYIWNQIASSLPMKFLCSEDCKGLCQKCGKNLNEGPCDCAVETGDLR
                     FEKLRELSLND"
     gene            complement(21952..23148)
                     /locus_tag="VFD79_04055"
     CDS             complement(21952..23148)
                     /locus_tag="VFD79_04055"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HZH93253.1"
                     /translation="MKILVMNCGSSSLKYQLIDMDGEKVMAKGLVERIGIEGSKIKHD
                     TTGKERVVIEEPMKDHKKAIELVLQVIVDKDHGAIESMDEIGAVGHRVVHGGEDFAGS
                     VIIDEAVMDALNRNIELAPLHNPPNIMGIEACKELMPDTPMVGVFDTAFHQTIPAENY
                     IYPIPYEYYDKYKIRRYGFHGTSHKYVSLRAAELMNKDIKDLNIVTCHLGNGSSVTAI
                     EGGKSVDTSMGFTPLEGLAMGTRSGDIDPAIIPFLMDKEGMTFDEVNNMLNKKSGVLG
                     ISGLSSDFRDLEIAAEEGNERAELALKVFTNRVKKYIAAYAAQMCRIDVLVFTAGIGE
                     NSDVIRRMVCEGLECLNIRIDNDLNNGIRGKEAVLSKDLTSATIMVIPTNEELMIARD
                     TLGLVN"
     gene            23401..24645
                     /locus_tag="VFD79_04060"
     CDS             23401..24645
                     /locus_tag="VFD79_04060"
                     /inference="COORDINATES: protein motif:HMM:NF010191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HZH93254.1"
                     /translation="MKVAGLITEYNPFHHGHRIHIEKTRELTGASHLVAVMSGSFVQR
                     GEPAVVDKWTRAEMAVKSGVDLVVELPFTYAVQTAELFALGGIRILEAIGVTDYLVFG
                     SESGDIGILNDIATILAEEPPLFKGYLKEHLERGNSFSVSRSLALEEFLLHEKIHMSN
                     TSEILKKSNNILGIEYLKALKKTKSSITPLTFQRIGDGYREREIKSKIASATGIRHSL
                     KSAGPESVKDYMPDPSYGAIIKFLQEYGRVNGLENYSQIINYLLCFLGPEKIADLFDV
                     EVGLENRLKNLSLATSDVFQLIESVSTKRYTETRIQRLLIHMLLGISRKDVLEAYAVQ
                     PGYLRILGSNSKGFQIINSIKENSSLEIINKLSDSINKLDQVNKDILQRDILATDLYF
                     LGLNLNKPLRNMDFKISPYIQK"
     gene            complement(24676..25122)
                     /locus_tag="VFD79_04065"
     CDS             complement(24676..25122)
                     /locus_tag="VFD79_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HZH93255.1"
                     /translation="MDLLNLIDEVEDIVEAGTGVPLTGKVMIDRDELLDIIKEIRIKL
                     PDEIKQAAWIKEERQRIIDEAQKTSTAMISEAETRLEELIDREEVIKQANQRAEEIVR
                     RAELSSEEIRNGALEYADELLYEAQENFKELIQLLNDNRKELRSKE"
     gene            complement(25122..25607)
                     /gene="coaD"
                     /locus_tag="VFD79_04070"
     CDS             complement(25122..25607)
                     /gene="coaD"
                     /locus_tag="VFD79_04070"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HZH93256.1"
                     /translation="MRVIYPGSFDPVTNGHLDIIQRCSERFDKVIISVLNNTRKTSVF
                     TVEERIFLLRESTKDLDNIEIDSFTGLLTEYSKKKGCSTIIRGLRAVSDYEYEMQLAL
                     INRKTYPDLETLFMVSSTGVSYLSSSLVKEIASFNGEISCFVPKVVEEALREKYKRGT
                     R"
     gene            complement(25604..>26033)
                     /locus_tag="VFD79_04075"
     CDS             complement(25604..>26033)
                     /locus_tag="VFD79_04075"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HZH93257.1"
                     /translation="DLFAGSGGIGIEFLSRGSKKAYFIDKNPDSIRTIKKNLEHTKLM
                     ENAEVIKGDSRGVIKQFKPGEIAFDYIYIDPPYADVEMFHKILQLIADRGILNEDGLI
                     IVEHDKSMLLNNDYGNLEIFDNRSYGSKEMTYYRNRRVTI"
BASE COUNT         6644 a         6210 c         4248 g         8931 t
ORIGIN      
        1 catgttcatt tgctttgcta cttcctcagc aaagttttct tctttcttct caagaccttc
       61 tcccacttca tatctggtga atcttctaat aactatgttt tcacctattt ttgaaatttt
      121 agcagcaagc aggtccttga ccttaacact tggatcctta ataaatcctt ggtctagaag
      181 acagatctgc tcgaagaatt tctccattct accttcaaca atcttctcgg caacatgagc
      241 tggtttaccc tcgttaacaa ccttgttaag ttggatttct ctctcatgtg caatttcttc
      301 ctctgaaagt tcttctcttg atacaacctt aggattggct gcagcaacct gcatagccat
      361 gtccttgaca aactccttga attcatcatt ttttgcaaca aaatcagtct cagagtttac
      421 ttcaataaga actccaattc ttccaccgtg gatgtatgct tcaacaagtc cctctgatgc
      481 taccctaccg gatttttttg acgctgatgc caggcctttt tcccttagat agtcaatagc
      541 cttatctata tcaccatctg ttttttcaag agctttttta caatcaagca ttccagctgc
      601 tgttttttct cttagttctt ttactaaaga agcactaata gccataatat acctcctata
      661 tcattaaaac ggtaaggaat acagataatt ctgtaatcct taccttcttc ttactcttct
      721 gtagcttcta cgtttacctc agattcgttc tgctcaccct gctttgcttc gataacagca
      781 ttagccattg tctcagtcaa cagttttaca gctctaattg cgtcatcatt accaggaatg
      841 atgtaatcaa tttcatctgg atcgcagttt gtatctacta tacctataac aggaattcca
      901 agaatatgtg cttcctgtac tgcaattttt tcttttcttg ggtccacaac aaacaggaca
      961 tctggcaccc tggtcattgt tttaatacca cctaggaatt tctcaagtct ttcagcttca
     1021 tgtctcagct taattacttc tttctttgga agaacttcaa aggttccatc ttcctccatt
     1081 ttttcaagct gatgcaatct gtcaattctc tttctgattg tcttgtagtt tgttagcatt
     1141 ccacccaacc atctctgatt tacaaagtgc atgccacatc tttttgcctc attctcgatt
     1201 gcttcctgtg cttgtttctt tgttcctaca aaaagagctt ctccgccatt ctcaacgact
     1261 tccttcatta cgttgtatgc gtcgtcaatt ttcttgacgg tcttttgtag gtcaataatg
     1321 tatatcccat ttctttctgt aaagatatac tgtgccatct ttgggttcca tcttcttgtc
     1381 tggtgaccaa agtgaacacc tgcttccaat aaacttttca ttgatactac tgacatactt
     1441 tcacctccat gttattttcc tccatctgta tccactatct cgggactcct aagagcacac
     1501 ccctaaatat caacagatgt gtgtaatcac catagtatta taccacagtg aatttaccat
     1561 tacaacgatt atttgatcct tttcacttct tttccataaa tatgacactc cttcaggagt
     1621 gtcatcagtt tacttcctct ttaattcatc aagcaatttt tcattaagta tcttgatgtg
     1681 agttcccttc atacccaggg atctggactc tataacacca gcactctcga attttcttaa
     1741 tgcgtttacg attacagatc tggtaatacc taccctgtcc gcaatcttac tggctacaag
     1801 caggccttcc ataccatcca actcatggaa gatgtgctcc attgcttcct tctctgaata
     1861 tgaaagggtt cctattgcca tttgaacaat tgatttcttt cttgcatcct cttcaatctc
     1921 ttcagtggtt gcccttagaa tttccattcc tacaactgtt gcactgtact cacaaagaat
     1981 caggtcttca actgtgaact ctttgccaaa tctggcgacc actaaagtcc ccaatctatt
     2041 tccacctgag ttgatgggta caacggtgga gatcttatct ggataaccac actctgtcac
     2101 atggtcaaaa acacagtcag taacttcctt gatgttctca cttgtttcaa ttacctttaa
     2161 tagctcttcg ttgtatttct ttggaaatct tctttccttc accacctcag aattgatcac
     2221 atcacactca aatccatctg ataaggcgta gccaagtacc cttccctttc tgctggcaat
     2281 gtagacattt gcctcaagga tgtcagataa tatcttgctg agatcagtga atgatacagg
     2341 ctgtgaacca tagctttgta aagtcttatt aagtcttctg gttttcttta acaaactctc
     2401 cattttttcc tcctactaaa ttatttattc ttagaacaat tcagaatatt caccatttat
     2461 atataagata ctatatccgg agaaaaaagt acagttgttt ttttgatttt tttaaatatt
     2521 tttactatac ccacaatttg aatctacaca ttttacagtg gttctacttt ttagacgttt
     2581 tatcaccata ggcccgttac aatcagggca cttttcctta acaggctcat cccaggaaac
     2641 aaaatcacag tccgggtagt tgttgcagcc atagaaaacc ctgccttttt ttgatctttt
     2701 cctgactaca tcgccaccac acttggggca aggcacttct atcttgtcaa ctataggttt
     2761 tgtgttctta cactcagggt atcctggaca agcaagaaac ttaccgaaac gtccgtgttt
     2821 gaccaccatg ttccttccgc atttttcaca gatcacatcg cttacttcat cttcaatttc
     2881 gattttgtca atttcctctt cagctattac aagctccttg tggaagtcct tgtagaaatc
     2941 tcttacgacc tctttccaaa aggtacttcc agatgcaatg tcatcaagct catcttcaag
     3001 attagcagtg aatttttcat ttaccacatg ggtaaagtac tcagttagaa gttcgtttac
     3061 caataagccc aactccgtag gatagaagga tttcttctcc aacacaacat atcctcttga
     3121 cagtatggtt gatattgtcg gagaataggt acttggtctt cctattccca tttcctccat
     3181 cagcttgatc aatgatgctt cagtaaacct ggctggtggt tgggtgaaat tctgcttggg
     3241 ttcaagctta tccaaatcca gaatatctcc cttattcatt gtaggaagtg ccatatctga
     3301 tgtttcctca tcagttgtag agtaaatcgt aagaaatcct ggaaatacaa gctgagaacc
     3361 attggatcgg aatacatact gtccgttcat gattctaaca tttagggtat tatatagggc
     3421 ttccttcatt tgagacccca taaacctgct ccaaatcatc ttgtacaacc tatattggtc
     3481 cttgttgaga taatccttta tatcatcggg tgaatggtca atatatgttg gtctaattcc
     3541 ctcatgggca tcctgtgttt ccttcttgga tttattgctg tagcttctac cgccattgct
     3601 gtactcttta ccaaatttgt tttcaatata tgaccttgca actgacatcg cctcattgga
     3661 tactctggtg gaatctgtcc tcatgtaagt tatcagaccg gtaatgccac cttttttcag
     3721 gtctattccc tcatatagct gctgagccac catcatggtc ttcttggttg aaaaacccag
     3781 ttttttagat gcgtcctgct gcattgtact ggttgtatag ggtggatatg gatttctctt
     3841 tctcttgccc tttttaacct cgttaacaat aaactcattc ttcttaagtt ccttaaggat
     3901 tgaatccgat tcagatttgc tatttatatg aagtttttca tccttaccgt ttttttcaac
     3961 tccataaaag ttggattcga aaccttgctc atccttgttt aatagagcct ttattgtcca
     4021 gtactccaca gggataaatt catcaatttc agattctctg tcgcaaatga gctttacagc
     4081 cactgattga actctaccag cacttaatcc cttttttatc tttttccata ggaggggact
     4141 aattttatat cccacaagcc tgtccaaaat tctccttgcc tgttgggcat ccacaagatt
     4201 cttgtccagg ttacgggggc tctttattgc ctgcttgatt ctttccttag taatctcatt
     4261 gaactcaacc ctgtactttt cgttttcatc caggccaaga atatatgcaa gatgccagga
     4321 tatggcctcc ccttccctgt ccgggtcagt tgctaaaaaa accttgtcag cctttttggc
     4381 ttcctttttc agatccttta taagtggacc cttccctctg atgtttatat agtcaggctc
     4441 gaagtcattt tcaatttcta ctccaagacg gctcttggga agatctctta catggccaac
     4501 tgaagcaacc accttgtagt ttcttcctaa tatcttactt attgttttcg ccttggccgg
     4561 ggactcgact attaccaaat atttcgacaa tgattacacc tccgaagcaa tccggatact
     4621 aaacaattgt aaatatcctg ctgctcattt cctttattat acctttcaac tctaaaatag
     4681 taagagtact tattacggtc tgtatgtcca atcctgattt taaaactatt atgtcactat
     4741 gtgcaggtcc ctccttaagg aattcaagca cacgcatttc tgtctcactg aggtcaattt
     4801 catttaatct atcctcatct tttgtttttt taattaactg cagttccctt acctcttcca
     4861 gtatatcatc catgtcaagt agtggctttg ccccatcttt tataagtagg ttggttccct
     4921 gactataaat gctattgatg ttccctggca atgcgaatac gtccttacct tgggcaaggg
     4981 catgatgggc agtgatcaag gaccctgact tctgttgggc ttccacgact attattccca
     5041 ggcttagacc agagattatc ctgtttctca tgggaaaatg aaaattgtag ggctgggcac
     5101 caggaggaaa ctcacttata agagctccat tatctgcagc atccttatat aatctgaagt
     5161 tcgattttgg ataaacgttg tccaagccgt ttccaaggat accaatggtt ctccctccat
     5221 tctccaaggc agtcctatgg gcaatggaat caattccagc agccattcca ctcacaatgg
     5281 tcactcccat gttagtaagg tctcgggcaa atctttcagc agcccacttg ccataattgg
     5341 ttgcttttct ggaaccaaca atacccattg cgatatggtc tctgggttga agtttcccct
     5401 ttatgtaaag tacaagtggt ctatcaggaa tttcagaaag ctcctctgga taggtattgt
     5461 cacaaactgt gacaacatct ataccaaggc catcaagctt ttccttgtat tcttcaatgc
     5521 tggatttgaa atcagacttg gcaattcttt tcaaaatacc actgcctaaa agtcccaagt
     5581 tttcaagatc actgtggtta aaatcatgga tttcacttat atctcccaag tatgatctta
     5641 acttatcaat aacatcacta tagattccaa ggctatttaa ccagataaga gtgtctcttt
     5701 ccaagttcat aaattacccc caatattttt tgtctaggct tctatattgg attgcttcca
     5761 gaagatgttt gtcatcaata ttttctactc catccaaatc tgccagtgtt ctggcgacct
     5821 tcaatatttt attataggtt cttccactga atctgagctt atcgaatgcg cttttcatta
     5881 ttttttcact ggatggtgtt aacttgcaga atcttttaat ttccttgtta cccatttgtg
     5941 agtttgtgta tatattcaac tctgagaacc tatctatttg ggtttgtctt gctcttgata
     6001 ctgattttcg catgtcatct gaagatcttg attgaatatc tgtgtttaac tcttcatatt
     6061 taaccggcaa tacctctata tgaaggtcaa ttctatcaag caacggatga ctgatcttcc
     6121 cgagatacct atctatctgg ttctgattgc aggtgcattg atgcatagga tctccatagt
     6181 atccacatgg acatggattc atgcttgcta caaacataaa gtccgatgga tatgtcagag
     6241 aagcatttgc ccttgaaata tgtacaaatc cgtcctctag tggctgcctt agaacctcca
     6301 aaacactttt ttgaaattca ggcagttcat caaaaaatag gacaccattg tgagccaagg
     6361 aaacttctcc aggctttgga atcctgccac cacctattat tgatacccca gacgatgagt
     6421 gatgcggtgc tctgaatggc cgctccttca ttagtctgct ttttctcaaa agccctgaaa
     6481 tactgtatat ttttgtaact tctatggatt cttcaaatga taggtgtgga agaataccag
     6541 gaattcttct agcagccata gtctttcctg aaccaggagg tccaatgatc agaagattat
     6601 gccctccagc agcagaaacc tccattgcac gcttaaggcc gtcctgtccc tttatgtctg
     6661 agaagtcgta ctggctatcc ccagaagtat cctctgcctt gatttctgca atgtatggtc
     6721 ttattatgtt ttcaccgttt aaatgattaa tcaggtcaaa aatattctcg acaggaataa
     6781 tatctacatc ctcaattatt ccacattcct ccctattgcc atagggtatg atacaccttc
     6841 tgattccaag atttcttaag gatattatca ttgccagggc tccatctatt gggtttacag
     6901 tcccatccag ggatagctca cctagaaatg ccacttcctc cgaatcaaat ccttccacta
     6961 aaccatctga aatcagaatt cctgcagcaa ttgccaaatc catctgagag ccatcctttt
     7021 ttaggtttgc aggtgctaga ttaacagtga tcctacttaa aggaaactca aatccactgt
     7081 ttcttattgc agttctaact ctctccttgg attcctttat tgcagcatca ggcagtccga
     7141 caattgtaaa catgggcata cccctagaca ggtctacctc aacctccaca atatgtccat
     7201 ccagtccttg caacacacaa gtcttgactt ttgaatacat agaacctctc ccttgcaagt
     7261 tgatgtgtac tagaaatgtt tattccccat tgagaataaa attatcttat tttacaatga
     7321 aaaagcattt tcaaagtggt tgattatttt tttctccata ggatatactt caatgacatc
     7381 aaaccttacc tgaacatcat acatcttgtt tttctgaagg tacatcaggc aggcatttgc
     7441 tattttcttt tgtttccttg aattgacgga ttcatatgca tatccgtaat cattgcccat
     7501 tctggttttt acctcaataa aagccaatat atccttgtca taggcaatta tgtccacttc
     7561 acctattttg ttgcgataat ttgtttccag aacactgtag cccttatctg tcagatagtt
     7621 tagggctagt tgttcgccta ttcttcctgt tttgatgtga gatttcatag ctatcctttc
     7681 agaatgtttt ttaggaacgt catcctgtgt ttattggtag gaccaagttc aacaagcgca
     7741 tccctgtgat tttttgttcc atatcccttg tttttggcca ggtcatagcc tggatgcacc
     7801 atatcccatt cctgacacat cctatccctg taaacctttg caaggatcga agcggcagaa
     7861 atgccgtgaa caaggttgtc tccatcaatc acggcttttt gtgggatatt tgattcaacc
     7921 tcttcagcat ccaccaagat gtaggatggg ctgattctat ttccttcctt atcccttaga
     7981 ttttgcaatg caagcttcat agccaatctt gtagcttgct taatgttgat tttgtctatg
     8041 atttcagggc tgaccactcc aaatccataa gctacagcct cgtctcttat tatgtcaaag
     8101 agcttttctc tctttttctc gcttaatttc ttggaatcct ttactccatc aatggtcaat
     8161 ccagctggca gtatcactgc acctgctact acatcaccat ataggcagcc cctaccaacc
     8221 tcatctatgc atgcaatatg cttgtggcct tcaaaatata actcttgttc aatttcaaac
     8281 atactattca cctcttttgg ggtactcaag agtaatttta cccatcattc cccttctaaa
     8341 ctcatccaga ataattctac ttaccttttc gtaatcaaag gttcccccct tgagaaggca
     8401 gcccctcttg gcagcgattg cttccatagc ttccaaaggt tcacagtcct tggacatttc
     8461 gatattgtac ctgtcaatga gtgcctgtga cttaacaatc atcagttttt caacaagttt
     8521 aagggccaag gtttcagtat caagtatttc atccttgatt gcacctgtaa atgcaaggtt
     8581 tattccaacc tgttcgtcct caaatttggg ccacaagata ccaggagtgt ccagcaactc
     8641 tattccgccc tttgttttta tccattggtt ggttttagtt atgccaggtc tattacctgt
     8701 ctttgcactc cttctcccgg ctagactgtt tatcaatgtt gactttccaa cattgggtat
     8761 accaagaatc atggctctaa ttggttggtt aattatacct ttcctggcat tgttaaccct
     8821 tttttcatcc attacccttc tggcttctgt cacaagatca tcaacaccct tgcctgacaa
     8881 ggagtttaaa agtatggatg atatgcccaa gtttttgtaa tgatcctgcc actgacggtt
     8941 tgcatttgca tcagccaggt ctgacttgtt tagaagtata attcttggct ttcctccaac
     9001 cagggattca ataactggat tttggctact gtatggaatt cttgcatcaa ggagttcaaa
     9061 tactatatca atcattttca gattcttctg tattgactcc ctggtttttt tcatatgacc
     9121 tggataccag ttaatgttca tactcatatt aacacccact taaataaaaa ttgggactga
     9181 aacagtccca ttaataaaat cttttttctt taactttagc agatttacct tgtctttctc
     9241 ttaggtagaa cagcttagct cttcttactt taccttttct agtaacaact agtttttcaa
     9301 ttcttggtga gtgaattggg aaggttctct ctactcctac tccatatgaa agtcttctta
     9361 ctgtaaaggt ttcccttgct cctccacctt gaacctttaa aacagtacct tcgaaaatct
     9421 gaactctttc tcttgtacct tctatgactc tgtagtgaac ctgtactgtg tctccaacgt
     9481 tgaactcagg gagatcattt ctcaattgtt catcttcaag cattttgata atatccattt
     9541 tgtatcctcc cttctaccta gacgttcttg ccctttaagc agaggaccgt ccttacttat
     9601 accttaggta ttatagcata ccggaacatt caattcaact atcttttccc aatcatcttc
     9661 agcaatgaat ccattagatc aagctggtcc ttggggtttt ctatttcctc caacatatcc
     9721 ggtcttttca taaatgtagt gcgtagtgca cttacccttc tccaaatgtc aattttcgag
     9781 tggtccccac ttaggagcac atccggtact ttgaaaccct taaatactct tggcctggtg
     9841 tactgtgggt attccaatag tccttcgtag tgagactcaa tctcgtagga ttcttcagat
     9901 gataaaaccc caggcattaa cctgcttatt gaatcaatca ataccatggc aggcaactct
     9961 ccaccagtta aaacataatc ccctattgag atttcatcat caacatagtt ttcgattatc
    10021 ctgttgtcaa caccttcgta atggccagct aaaagtatga gatggtcttc attagccaac
    10081 tccttgacca atgattggtt caggattttt ccttggggtg aaagatatat cacccttgaa
    10141 ccaacccttc tacaagactc tatggctgaa tgaatgggtt caggtttcat aaccattccc
    10201 cttccacctc cataggggta atcatccacc ctcttatgct tgtctgtgga ataatccctg
    10261 atattgatgg cattaacatc tatggcttca gaagcaattg ctcttcctac aatgctatag
    10321 tcttttagca tgtcaaatat ttcagggaaa agggtcaaca catcgatttt cattcaatca
    10381 ttccttcaat aggatctaca attattattt tttcaacaac atccacagaa gctataaagg
    10441 cttcaacagc tggaattaga tattctttgc cggtgtctga agttactaca taaacatcat
    10501 tagctgagtt ttggattaca tctgttaact ccccaataaa ggtttctgat ttatcataga
    10561 ctctacagcc aacaaggtct gaaataaagt agtggttttc aggcaacctg accttgtcct
    10621 cttcatctac gtaaaggtac tcatccttaa aatccaaaac cttgtttatg tcctcaagac
    10681 ctttaaacct caataggacc atgcccttgt gaagcttcac accactaacg tggaccttga
    10741 tcttattctc gccaacatag gctttctcaa gttcaaaaaa cctttcagta gagtctgtca
    10801 gaggaaacac cttcaggtct ccctttattc cgtgtgttcc cgttaacttg ccaattactg
    10861 tatattccat gcttacaccc gccaatgcgt caattattga atgatttcaa caaccactct
    10921 cttgttgtcc ttgatagcag ctgccttgac cacagttcta atggcctttg caattcttcc
    10981 ctgcttacca atgattttcc ccatatcatc aggtgcgact ttcaattcta tgataattga
    11041 ttgggatcct tccacttcgt ttacactgac ctggtcagga ttatctacaa gtgcctttgc
    11101 aataaattct accaattcac ccattatctg cacctccgag attattcagc agtttcttct
    11161 tcagtcgtag tttcttctgc cttatcgtac aaaccgctat tcttgaatag tctttcaact
    11221 gtctcagtcg gttttgcacc attcttaagc cacttagcag ctttctcatt gtctatcttt
    11281 actgtctttg gttcagtaag aggattgtag tatcctagct cctcaatgaa tctaccatct
    11341 cttggtgctc ttgagtcagc tacaacaatt ctatagaaag gagtcttttt tgcacccatt
    11401 cttcttagtc ttatttttac agccatttat ttcacctccc attatcactt agtatctatt
    11461 tagaaaaagg ggaaacccat cttccctttt ttcttcgatg gtttactcat gtcagaaaac
    11521 ttcttcatca tcttttttgt ttccttgaat tgctttagaa gtttattgac atcctgtaca
    11581 tttgtgccgc taccggctgc aattctcttt ctcctgctac tgtcaatcat atctggattg
    11641 tccctttcct ttttggtcat ggactttata atagcttcta ttctagcaag ctcactattg
    11701 tctacattta aacccttaag cgcctttgaa ttcatgcctg gtatcattcc aagtatctca
    11761 tccaatggtc ccatgttttt catctgttca agctggtcta gaaaatcgtc aaatgtgaac
    11821 tgtgcagttc tcattttctt ttccagctcc attgctttct tttcatcaat gtttgattgg
    11881 gctttttcaa taagacttaa tacatcaccc attccaagaa tccttgagga cattctgtcc
    11941 ggatgaaagg gctccagctg atcgagtttt tcacccatac caacaaattt gatgggcttg
    12001 tcggttactg ccctaatgga cagggccgca ccacctctgg catcgccatc aagttttgtg
    12061 agtacaactc ccgttatgct aagctggtcg ttgaatgcct gggcgacgtt tactgcatcc
    12121 tgacccgtca tggagtccaa caccaaaagt atgtcatcgg gatcggtcgc ttccttgatc
    12181 tcagttattt cctgcataag gttctcgtca atatggagtc ttcctgcagt atctattatt
    12241 attacgtcat ttccattttt cttgccatgc tccatggctg cttttgcaat atccaccggg
    12301 ttatgcttat cacccattga gaaaactggt acaccgacct tctcacctac cacttcaagc
    12361 tgtttgattg ctgcaggcct atagatatca caagctacaa gtaagggtct cttgttttgc
    12421 ttctttaggt tgttggcaag cttaccggat gtggttgtct taccggcacc ctgcaaacca
    12481 cacatcaata ttaccgtagg aggcgttgaa gccatattca gtttgacttc cctgtctccc
    12541 ataagagcag tcaattcatc atttacaatc ttaataacct gttgcccggg agtcaggctt
    12601 tcaagaacct cagcacccag tgctctttcc ttgattttct tgataaaatc tttgacaacc
    12661 ttaaaattga catcagcttc caataaagcc aatctaactt cccttagagc tgcgtccagg
    12721 tctttttcac tgatttttcc cttgcctttg agctttccca aagcattctg aagtttttct
    12781 gataaacttt caaataccaa aatgaaacct cctatatcta cagtaaaatg tcagaaatca
    12841 ggctttctat catcttagcc ttttcgacaa tttcatcgtc agatgacttt tctccaataa
    12901 tacaaatttc acgtgctaga tttctcgact cacgtatggc ctcttggctc tcataaaact
    12961 tatctacaag tcctaacttc tcctcgtact taagcagctt atcctctgcc cgcttaagag
    13021 tatcaaatac tccctgcctg cttatttgga gcacttctcc tatttcagac agtgaaaggt
    13081 cattggagta atacaaatca ataacctggt attgcttggg acttaagagc tttccataaa
    13141 agtcgaacaa tatgccaatt ctaaccagct tctccatttt atcaccaact tattatactg
    13201 tcaagcacta tccttgacag tataataata ttttaaatct cataatttgt caacacctat
    13261 tcgaatattg catctataaa gctttcagta tcaaatggct gaagatcctc aaccttctct
    13321 cccacgccaa taatctttat gggcaacttc agctcagtct gaagtgcaat ggccactcca
    13381 ccttttgcag tcccgtctag tttggtcaag gcaaccccag ttatgttggc aacctccttg
    13441 aatactttgg cctgatttat agcattttga cccgttgtgg catcaagaac caggagcact
    13501 tcctttgtag cttctccata ttccctctca actatcctga atatcttatt tagttcgttc
    13561 attaggtttg atttgttgtg aagcctacct gctgtatcac aaatcaatat atcagccttc
    13621 ctggccttgg ctgcctggat tccatcgaat atcacagcag caggatcaga accctccgta
    13681 tgggaaatta tctcagctcc tgccctcttg ctccattctt ccagttgttc tatagctgca
    13741 gctctgaagg tatctccagc tgctattagg actttcttac cattctgctt gaatccatat
    13801 gccaacttgc caatggtagt ggtcttgcca acaccattaa cccctactac caaaagcact
    13861 gatggatgtg gtagtaaatc aagatcgttt ccaggaaccg actcaatcat aagcttctta
    13921 atttcctcct tcaggagtcc ctttacatct tttggatcac ttaccttctc ctcacgaacc
    13981 ctttccctaa gtctatcaat gatcatcatg gttgggttga cgcccacatc cgcagatacc
    14041 agtacatcct caaggtcatc aaataactcc tcatccactc ccttgtatcc gctgataatg
    14101 tcctcaatct tgttgctcat gtccttacga gtcttggcta ggccattttt tagcctatca
    14161 aggatgcctc ccgacactgg gcctgcatca tcaacttctg cctcatcatc aagaccaagg
    14221 gcatcctcaa ccatgctgga agtcttctcc tcgttgccat cttcaagatc atcatcttca
    14281 tcttctattt caagaccatt atctgtgttt tgggactcaa gggtctgatt gtattcctca
    14341 ctcttcctct tgtctttttt gaaccaatta aacatatttc tcctcctagc ttgctatttc
    14401 gtcgtagtcc ttaagtttta gtgaaatcat cctgcttacg ccttcctcct ccatggttac
    14461 gccatataag acatcagcaa tttccatggt ggttttccta tgtgtaatta ggacaaattg
    14521 ggtgttgtcc tcaaggctgt tgaggtatga tgtatatctg ctgatgtttg cctcatcaag
    14581 ggctgcatca atttcatcaa gtatacagaa aggtgatggt cttgttttca gtatggcaaa
    14641 caagagtgca acggctgtaa gagatttttc tccaccagat aaaagtgtca gactttgaag
    14701 cttcttacct ggaggttgag ccattatatc tatccctgca gtcagtatgt catcaccatc
    14761 ttcgatctca agcctagctt tccctccttt gaagagcaca gaaaaggttt cgctgaatct
    14821 ctcattgatt tcctcaaagg actttctgaa ctggaccctc atctttttct ccatgtcctt
    14881 tacaacggac tctatgtcct cctttgcccc attaaggtcc accacctgtt tgtttaggaa
    14941 ttcgtacctt tccaggacat tgtcatattc ctctatggag ctaatgttta cattacccag
    15001 ttccttgatt ccttgcttca attcagaaat ggttttcttt gtccccctta tgtcctcaat
    15061 gtcagaatat attttttcag cctctgccag agtgatttcg taatcatttt ccagcttttc
    15121 tagaatattt tcaatctgca gctgagcctt ggcctgatcc acttcaagct tgttcaactc
    15181 cctttcaaca gaagtctgac gcttggtcag ctcccttaac ctatcctgaa tttgatagaa
    15241 ctccttcatt agtttttcct tttccccgac cttttccttc aataagagag attcttcttc
    15301 ctctgtcgtt gattgtctct ggatatattc acacaggctt ttcttttcaa gggtgttttc
    15361 tgatatttcc atctcaagtc tgtcaagttc tgtttccttt agcttaatgg actccctggt
    15421 ctctgaaatg gatagttcat tctcctgtat tctttccttg aaagtaccca atgatttctc
    15481 cagggcgttc agcttgattc gagtttcagt aatatcgtta tccaatatgg ttctgttgtt
    15541 tttgtattca tcgagagcct cctgcatgcc agccaggtct gtataaagct tgtcatgact
    15601 atcctttaag gcatcctggt ctgacttaag ctcagctttt tttgtttcaa gctccttaag
    15661 ttcatccctt aatcctgata tttcggttct tttagaattg attgactcat tttgaatttc
    15721 gtactgatac tccatattgt ccatttcttt ttcaagaaca gtagttttat caattaaacc
    15781 cgtcttttcc tggattaagt tatcgtacgc tttccccagc tggttcaatt ctgattcaat
    15841 ggtcactatg gctttagttt tagcttcaat ttttaacttg atattcttga tttttatttc
    15901 caagtctgcc aaagtatgtt ttctattcaa aatgctaatt gactgatttt ttgtactccc
    15961 tccggttaga ctgcctcccg ggttgatgat ctcaccctcc agagttacaa tcctggattt
    16021 gaaaccggat ttttttgaat aattgatagc atcttccagt gagtttgcaa gtattgtcct
    16081 gccaagcagg ttcataaaga ttttgctata cttttgatca gcttcaagga tgcttgaagc
    16141 cgctcctatg agaccatatt ctttcctatc cctatcctct atgctgattt cccatcctga
    16201 aaccgtatta aggggcagga atgttgccct acctttcttt tcagatttaa ggtagtcaat
    16261 catcctcctg gcactgacgt catccttgac aactatgttc tgaattgatg agcccaaggc
    16321 cacctcaata gccttcatat actttttatc cacggtaaac aggtcggcta cggttccaat
    16381 aaacccatct cctagtttgg tatctttgtt gattgcctta actagtgact taacactgtt
    16441 gtagtaaccc tcgtattctt tttccatatt cctataaagc ttttgagatg atatggaagc
    16501 atccagttcc ttctgatcct cctggagctt ggcttgttca gagcctatcc tagctttaag
    16561 cttacttgcc ttagcttcaa cttccatgat tttttcttcg ttaatcctct ttgcttcaag
    16621 gagactctct agatctctac ttttattttc caacccattt ttcaatcttg aacatttgtc
    16681 ggcaattgaa ttctcatcct ctgtaagaat atcaagcctt ccctttatgc tatcctcaaa
    16741 ggaagcaatt gtattgcttt cgctcttctt gtccgacagt gatgaataca aggtcataag
    16801 ctgattcttt ttgtctgcaa tggttttttc cagactgagt gttcttccat caagggcttt
    16861 tctttcttca aacttcccat ccaggtgtgc ttttaacatg gctacttcat tatccaacct
    16921 atcaatttca atagtggatg tatccttcct cgtgtcgccg gcgtgaagaa agtccttgcc
    16981 cttggctatt tcttcattta accttgcaac ctccttctta agatgggact ccctttcctc
    17041 caatatcttg acccggttct ttgaactctc aaattcctgt atactctcca tcttgagtgt
    17101 tcttttttcc tcaataattc tctccagact ttcaatactt gactttagag tagagtaccc
    17161 ttcctcaacc tgggtgctat cagcctcaat tatggctttt tcactgtcca agtcaatcct
    17221 cttaccttct gcatcatcca gtttggtctt caaacgattt acttcatata ctgaaagact
    17281 ggagtcaagc cttcgcaatt gcccatatag cttctgaaat tccttggcct tctctgcctc
    17341 ggtctggaga ttctccttct ggttttttat ttcgtgaagc aggtcaccga ttcttgtaag
    17401 attagtttga gttctttcaa gttttcgcaa tgcttcctgc ttcttagact tatactttac
    17461 aataccggct gcttcctcga agatatttcg tctgtcctca ggcttgtttg agaggatctc
    17521 atctattttc ccctgtccga ttattgaata gccttcctta cccacacctg tatccatgaa
    17581 aagctcacga atgtccttga gtctgcagga attcttattt atgtagtatt cactttcacc
    17641 tgacctgaac atccttctgg ttactgcaac ctctctatag tctatgggta tcctgccagt
    17701 ggtgttgtca aaaactatgg tgacttcaga gtagcctagg gcctttcttt tttcagtacc
    17761 tgaaaaaatg atgtcctcca tccgggtacc tcttaggttt ttcacacttt gctcaccaag
    17821 gacccatctt atggcatctg caatattgct tttgccactt ccatttggac cgacaatagc
    17881 cgttaccctg tcattaaaga caacttcagt tttttcagca aatgatttga atccttgaat
    17941 ttctactttt tttagataca ttgtatacac tccattccgg cctcaatgac catcccctac
    18001 aaaggttccc gtttgggaac ctttataagg gattattatt aatgtataat ttcgtttcct
    18061 cacctaagtc aatgaccctc tcgtcgtttt ccatagatag tattattcta ctatcttttc
    18121 ccctgaaagg aattattctg acttttgtca gtccgtagtc ctcaatcagt ctctccttgt
    18181 ttttgccatg ttgaccagca ataattgatg tcctggaatt ggcacatttg attgtcaagc
    18241 tcttaccctt cgtctcaatc ttattcctgg ctatgtattc ccttagaagt aaagagaaca
    18301 cttgctcctc aaccaattgt ctgaaggatg gatggaaggg tcccgcaaca acatcccttc
    18361 ccatttgaat gttttccgtt ggttgaagcc cgattctgat tacagggata tcatgggcag
    18421 taaaggccat taatataatg gcagctgttt cgatggcttc ctctatgctt aggggagcat
    18481 agagtccatc cttcatctgt ctttcaagtg gggtattcct aatgacaaga gtgggataaa
    18541 ttctgatcat gtctggaccc atttcaagta ctttttttgc agtttcaagg ctcttggcct
    18601 gactatctcc cgggaggcca agcataatct gatgtccaag cttgaatccc cactcccgga
    18661 taagcccgct tgatgcatat acatcctcat ccctatggcc tctttcgctc atggctagaa
    18721 cttcatggtc aagtgactgg actcccaact ctatagtatc aacctgaaaa tcctttagaa
    18781 gtcccagaat atcctcatta atggcgtcag gtcttgtaga caaccttatg ttgtgaatct
    18841 tcccttccat tttatattca tagggaaccc taaggagttc tctttgaatt tccatctgaa
    18901 ttgcagtaaa gctcccccca taaaaagcaa cctcaataaa ggagtctctc ttgaaagtct
    18961 ctaaatggtc atctattatt cctttaacat cttcaactgt aacatcagtt gaaagtcccg
    19021 ttatctttct ttggttacag aaaacacaat catgtggaca tccaaaatgc ggaacaaaaa
    19081 ttggaatgat gtaatgtcta gtcataatct tctcccatta gaataagtgc ctcttttgct
    19141 gccatctgtt cggcttcctt cttatttcgc ccaattccag acccaataac atccccaccc
    19201 acagcgacat tcacatgaaa aagcttgttg tggtccggcc cttcctcctt gacaacactg
    19261 taaaccagct tttcacgagt tattttctgg agtttttcct gaagccctgt tttatagtca
    19321 acaaaaagtg accctttggc aacagctttc actatggtcg tctcaaagtt tttaagtaga
    19381 aattctctta cggtctcata gttgctatcc ataaaaatgg cacctgtaac cgcttcactg
    19441 gcatcagcaa gtatggaatc cctctccctg ccacctgtac tctcttcacc cttgcctaaa
    19501 agcaggaaag ctccaagatc gattcttctt gcagcgagag ctagagaagg ctctgatacg
    19561 accttggctc ttatttttgt aagctcacct tcaggataga atggatattt cataaacaga
    19621 tattcactaa cgaccaaccc caacacagta tcacctaaaa actccatcct ttggttgctc
    19681 ttacttgcat gcataccatg ttcgtttgca tatgaactat gggtcaaggc tgtttcaaga
    19741 agttctacat cctcaaagcg atagtttaga ttgttttgta tttcttcaag tttttctctg
    19801 tctaaatatc cgctcatcct tccacctcca ggcttattcc aattttccct ttatatagtt
    19861 cctggcatca ccgacagttt ttataagttc aacatcatcg tcctcaactt ccaggccgaa
    19921 ttcatcctca agtgccataa ccagctcaac aagatcaaga gaatcagcat tcaggtcatc
    19981 tctgaatgaa gtctccattg ttacgtcctt ttctggtaca ttgaactgtg ttgttattat
    20041 gcttattatc ttttctgtta tcatattaag cctccattat agtattttgt tctctattct
    20101 accaataaca tccttctcaa taaaagtctt tagttgtcca attccgttct tgaaggcata
    20161 gaagtccgaa ctcccatggg cctttacaat gggtttgact gttccaagta aaggtgcccc
    20221 tccatattct ctgtagtcaa gacgtttttt catgtttttc aattggggtt taagcatcag
    20281 agcaccaagt tttgatctgg tgtcagccac aaatgattcc ttcataagtg taaatatgga
    20341 tgacgccata ccttctgaaa gcttaagaac cacattgccc acaaatccat cgcaaaccat
    20401 tacatctgca acaccaaatg gtaaatctct tgcctcgata ttaccaacaa aattcatatt
    20461 tgaatccttc aaagccacaa atgtgtcctt ggcaagctgg tttcctttag tatcttcagt
    20521 cccaacattt ataagtccaa ccctgggttc cttcactccc atgacctctt ccatgtatat
    20581 ggatcccatc aaagcaaatt gctcaagata ctcaggcttc gtatccatat ttgctccggc
    20641 atcaacaagt agggatgctc cacttaaggt tggataaagc acagtcaatg ctgcacggtc
    20701 aatgccctgg attctcttga caataaagag tcctcctgcc agaagtgccc ctgtgtttcc
    20761 agctgatatg aagccgtcac cttcaccatc tgcaagggcc tgcattccct ttaccattga
    20821 gctgtttttc tttcgcctta tggcaagggt tgggttgtca tcgttggtaa tgacctcagg
    20881 tgcatccatg atctggattt tctctgctgg gtatgaatga ttttctaaat gctcctttat
    20941 aagtgactca ttgccggtga tcagaatgtc tataccgtat tcattaactg cctctactgc
    21001 tccctttaca atggcttccg gtgcattgtc tccacccatg ccatccacaa taatcctcat
    21061 acttatccct catttataat ttttgacggt atcctttgca atccaaaagg tatactcatt
    21121 atatccattt tagcacataa aaaaaagata gtgaagctaa tcactatctt tcttgtttac
    21181 tcgactacaa taacttccct gctcttgtaa tagccacagc ttctacaaac tctatgtggt
    21241 tgtttaggct catgacattg tggacattca acaactgtag ttttattaag tctataggaa
    21301 gacgctcttc ttttgtctct tctggcttta gaagttttcc tttttggtac tgccatttaa
    21361 aacacctcct taatcattta gagaaagttc ccttaacttt tcgaatcgca aatcacctgt
    21421 ttcaaccgca cagtcacaag gtccttcgtt aaggttcttg ccgcatttct gacacagacc
    21481 tttgcaatcc tcgctgcata aaaatttcat cggtagggaa cttgctattt gattccagat
    21541 gtaatcattg agatttacat acttgtcctt gtagtaaact agctcgtcag attcctcatc
    21601 ttcaattttg ttccctgatt cctcttccat caattttcct gaagctttga aagatatttc
    21661 attgctgctt tcagcaagac atcggtcaca tggtgcctgg taaaggtaat tccccttact
    21721 ttccatgagt atttctccat ccaccttata gaagttaccc tctatatgta ttgggtccac
    21781 aattgaagaa acatcttcaa ttggaaactc gtccctggac aggtcaaatg agaaactcat
    21841 aacctgcttt ccactgtcaa gaaaatctga taaatcaatt ttcatagttg tcacctctta
    21901 gactaacact actattatat atatgaggga cacctttgtc aaccactaac tttagttgac
    21961 caatcccaaa gtatctctag ctatcatcag ctcttcattt gttggaatga ccattattgt
    22021 tgcgcttgta aggtccttgc tcaagacagc ttccttgcct ctaataccgt tgttcaaatc
    22081 gttatcgatt cgaatgttaa ggcactcaag tccttcacat accattcttc taataacatc
    22141 tgaattttcg ccaatacccg ctgtgaaaac aagtacatct attctgcaca tttgtgcggc
    22201 atatgcagca atatatttct ttacgcggtt tgtgaatacc ttcaacgcaa gctctgctct
    22261 ttcatttccc tcttcggctg ctatctcaag atccctgaag tcactgctca accctgaaat
    22321 tcctaggact cctgacttct tgttcaacat gttgttgact tcatcaaaag tcatgccttc
    22381 cttgtccatg aggaaaggaa ttattgctgg gtcaatatcc cctgatctgg ttcccattgc
    22441 aagtccttca agtggtgtaa agcccatgct tgtatcaact gattttccac cttctattgc
    22501 agttacgctg gagccattgc caaggtgaca agttacaatg ttcaagtcct tgatatcctt
    22561 gttcataagc tctgcagctc ttagggatac atatttgtgg gatgtcccgt ggaatccata
    22621 tcttctgatc ttatatttgt cataatactc ataaggtatt ggatagatat agttttcagc
    22681 aggtatggtc tgatggaagg ctgtatcaaa tacccctacc attggtgtgt caggcatcag
    22741 ttccttgcat gcttcaattc ccatgatgtt cggtggatta tgcagtggtg ccaactcgat
    22801 gtttctgtta agtgcatcca taaccgcttc atcaatgata actgagccgg caaaatcttc
    22861 ccctccgtgt acaaccctat gtccaacagc gcctatctcg tccatagatt caattgctcc
    22921 atggtccttg tcgacaatga cttgaagcac aagctcaata gcttttttat ggtctttcat
    22981 tggttcttca attaccactc tttccttgcc agttgtatca tgtttgattt ttgagccttc
    23041 tattcctatc ctttcaacaa gaccctttgc cattaccttt tctccatcca tatcaataag
    23101 ctgatattta agtgaagaac taccacagtt cataactaaa attttcattt acagacctcc
    23161 taacaatatt tctatttcaa tagtattata tttgtgttaa aaaatcaaca gaagaaagca
    23221 aaacagaagc aaaacataac tccaataggt ggttatccaa taaatattga gtttgggaga
    23281 cagatccagt tttttttatt tgttaatact cccctgtagt gttattattt ttagtaaagc
    23341 agcacatatt tagaatgaaa tgttaactta aacccatttt tgcaggaaga ggtgacaaaa
    23401 atgaaagtag ctggcctaat aactgaatac aacccctttc accatggaca ccggatacac
    23461 attgaaaaga ctagagaatt gacaggcgcc tcccatttgg ttgccgtcat gagtgggtcc
    23521 tttgttcaaa ggggagagcc tgcagtagtg gataagtgga cacgtgcaga aatggcagtt
    23581 aaaagcggag tagatctggt ggtggagtta ccattcacct atgcggtgca aactgctgag
    23641 ttgtttgctt tagggggcat aagaattctt gaagcaattg gggttactga ttaccttgta
    23701 tttggcagcg agtctggaga tattggaatc ctgaatgaca ttgcaacaat attggcagag
    23761 gaacctcccc tgttcaaagg ttacctaaaa gaacatttgg agagagggaa ttcattttca
    23821 gtatccagat ccttggctct tgaagagttc ctgctgcacg aaaaaatcca tatgtccaac
    23881 acatctgaga ttctaaagaa atcaaataac attctcggga ttgaatatct taaagcatta
    23941 aagaagacaa aatcatccat aacacccctg acattccaaa gaattggaga cggctatagg
    24001 gaacgggaaa tcaagagtaa aatcgcttca gcgactggaa taaggcactc ccttaaaagc
    24061 gcaggtccag agtctgtgaa ggattacatg ccagatccat cctatggagc aattattaag
    24121 ttccttcaag aatatggccg tgtgaatggt ttggaaaatt actctcagat aatcaattac
    24181 ctcctttgct tccttggccc agaaaagatc gcagatttat ttgatgttga agtgggactt
    24241 gaaaacaggt taaagaattt atctttagcc acatcagatg tttttcagtt gatagaatct
    24301 gtatcaacta aaagatatac tgaaactcga atacaaagac tgcttattca tatgctttta
    24361 ggtataagca gaaaggatgt tcttgaggcc tatgctgtgc aaccaggata tttgcgaatt
    24421 ctaggaagca atagtaaggg cttccagata attaactcta ttaaggaaaa ctctagtctt
    24481 gaaatcataa ataagctttc tgattcaatc aataaactgg atcaggtgaa taaggatatt
    24541 ctgcagagag atattctagc aacagattta tactttctag gattaaatct taataaaccc
    24601 ttaaggaaca tggacttcaa aatcagtccg tatatacaaa aataagggac ccacttttta
    24661 aggagggtcc ctctactatt ccttgcttct cagttcttta cgattatcat taagcagctg
    24721 aatcaattct ttgaaatttt cttgtgcttc atagagcaac tcgtcagcat actccaaggc
    24781 gccgtttctt atttcctcag aactcagctc agctctcctt actatttcct ctgctctctg
    24841 gtttgcctgt ttgattacct cttccctgtc tattagctcc tctagtctag tctcagcttc
    24901 acttatcata gcagttgagg ttttttgggc ttcatcgatt atcctttgtc tttcttcctt
    24961 gatccacgca gcctgcttta tttcatcagg aagcttgatt cttatttctt ttatgatatc
    25021 aagcaattca tccctgtcaa tcattacctt ccctgttaat ggtactccag ttcctgcttc
    25081 tacaatatcc tcaacctcat caatcaagtt caacaaatcc attaacgagt cccccttttg
    25141 tatttttctc tcaatgcctc ttccacaact tttggtacaa aacaagatat ctcaccgtta
    25201 aatgatgcga tttccttaac aagtgatgag cttaaatacg atactcccgt tgatgaaacc
    25261 ataaaaagtg tctctaggtc ggggtaggtt tttctattga taagggccaa ttgcatttca
    25321 tactcatagt ctgatactgc cctaagtccc ctgattattg tactgcaacc cttctttttt
    25381 gagtattctg taagcaaacc agtaaaagaa tctatttcta tgttgtcaag atcctttgtg
    25441 gattccctta aaaggaaaat tcgctcttca acggtgaaaa cactggtctt cctggtattg
    25501 ttcagcacag aaattatcac cttgtcaaat cgttcagaac atctttggat gatgtcaaga
    25561 tgaccatttg ttactggatc aaagctgcct ggataaatca ctctcatatt gtcactctcc
    25621 tatttctgta gtaggtcatt tccttactac catagcttct gttgtcaaat atttccaagt
    25681 taccataatc gttatttaac aacatggact tgtcatgttc aactattatc agtccatcct
    25741 catttaatat ccctctatca gcaattaact gaagtatctt gtgaaacatc tccacatcag
    25801 cataaggtgg atcaatataa atgtaatcaa aagcaatttc gcctggtttg aattgtttta
    25861 ttacacccct tgaatctccc tttatcactt cagcattctc catcaacttc gtgtgttcaa
    25921 ggtttttttt gatggtcctt atggaatccg ggtttttatc tatgaaatac gctttttttg
    25981 accctctgct taagaactca atcccaattc ccccactgcc tgcgaaaagg tcc
//
LOCUS       DASRQF010000148         3111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1004176, whole genome shotgun sequence.
ACCESSION   DASRQF010000148 DASRQF010000000
VERSION     DASRQF010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004176"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..451)
                     /locus_tag="VFD79_04080"
     CDS             complement(215..451)
                     /locus_tag="VFD79_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93258.1"
                     /translation="MIKVRVYPGPFGKRGLLDDDGFILMPEETTVGDLLKTMGIPRIV
                     YNMGFYGLNYKKEPLDRRLYDGDTLSFIAPLAGG"
     gene            complement(461..2449)
                     /locus_tag="VFD79_04085"
     CDS             complement(461..2449)
                     /locus_tag="VFD79_04085"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HZH93259.1"
                     /translation="MKGYIGKVLFVDLSTGEFRDEVVADEVYESFLSGVGLGAYYLYR
                     NIPQGADPLGEDNMLGFVSGLLTGTGSVVTGRWLAVGKSPITGGWGDSNCGGNLSSAI
                     KRSGYDGIFFRGISENPVYLYVDDDGPRLMDASLLWGRDAVEAEEMLLEMHGGERKAA
                     VAVIGQAGEKRSLIAGISNDKGRMGGRSGLGAVMGSKHLKAVVLAGTGKIKAHDREEI
                     LKITKEYSDKVKNVSLPGIVKGSMLPMAGRLMEKAGGLMPQDGMMSIPMMKKWGTIFS
                     NTFGLPSGDAPVKNWGGSILDYHFSSYRNMNPDRIIEREERKYHCYSCVIGCGGVCNI
                     SDVTGGKFHTTHKPEYETVNSFGALLLNESLDSIYYINEIMNRAGMDTISAGNTIAFA
                     IECFENGTISREDTEGLELTWGNTGAIIALAEKMISREGFGDILADGVKLAAERIGKN
                     SYMYAINAGGQEPGMHDPRLDPILGIHFSADPTPGRHTVGAGLYYNYMRLWDFVSWAP
                     RISSRGSKGNKYEAIGETALKSVANSCIKQILDGSGGCLFAMISGLGNWRLFDYLNAA
                     TGWEKSPDEYMEIGKRMQTLRQMFNIREGIDPISFRMHNRIAGNPPIKEGPLKGRTVP
                     IEDMMKGHWKHFGWDEETGKPLGETIQGLDLPRLLNME"
     gene            complement(2542..>3111)
                     /locus_tag="VFD79_04090"
     CDS             complement(2542..>3111)
                     /locus_tag="VFD79_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93260.1"
                     /translation="FNSLLEKQKDERGEALVTTVLFDDDYELLHDRINIKGIKPITER
                     EYYVRGTTALLDAVGRTIQKIINAQRHTAPDEQAENVMFVIITDGMENASREYGYDRV
                     RKMINYQKAEYGWEFLFLGANIDAAETAKQFGISRDRATNYNPDGEGTRLNYQVLSDT
                     VSMKRTRKVIDDDWKDRIEKDYKEREKKQ"
BASE COUNT          712 a          888 c          551 g          960 t
ORIGIN      
        1 atttatctcg atgtcaagat attgttttta atcataatca tcagcttcca atacaacaat
       61 atgttgctcc agatgttcat caataaaaaa acaaggccct ttcgccttaa atcatgtcga
      121 tgggcctatt aaaaacttcc atattctatt gattttccag atcctcaata taatccttaa
      181 tttggtgaat ttctagcttc tgacttcaat aatttcaacc tccggccaag ggtgctataa
      241 agctcaatgt atcgccatca taaagcctcc tgtccagagg ctcttttttg tagttcaagc
      301 catagaagcc catattatat actatcctcg gtatacccat ggtcttaagc aggtccccaa
      361 cggtagtctc ttctggcatg aggataaaac catcatcatc cagaaggcct ctttttccaa
      421 agggtccagg atatactcta acctttatca taaaaccacc ctattccatg ttaagaagtc
      481 ttggaaggtc aaggccttgg atggtctccc caaggggctt tccagtttcc tcatcccaac
      541 cgaaatgctt ccagtgcccc ttcatcatat cctctatggg aaccgtccta ccctttaagg
      601 gcccttcctt aatgggtgga tttccagcaa tccggttgtg catcctaaag cttatggggt
      661 cgatcccttc ccttatattg aacatctgtc tcagggtctg catcctcttg ccaatctcca
      721 tatactcatc agggcttttt tcccatcccg tcgctgcatt tagatagtca aacagcctcc
      781 agttgcctaa tccggatatc attgcaaaca ggcaacctcc tgagccgtca agaatttgct
      841 tgatacagct gtttgcaact gacttgaggg cagtttcacc aatggcctca tacttgttcc
      901 ccttggaccc cctgctgctt atcctgggtg cccaggatac gaagtcccat agtctcatgt
      961 agttgtagta gagtccggcc ccaactgtat gccttcctgg tgtgggatct gcagaaaaat
     1021 gtatacccag gattggatcc agtcttggat cgtgcatgcc tggctcctgt ccccctgcat
     1081 ttattgcata catatatgag ttcttcccta tcctctcggc tgccagcttc actccgtcag
     1141 ccagaatatc tccaaagcct tcacggctta tcatcttttc agcaagggct atgatggctc
     1201 cggtatttcc ccatgtcagc tccaggcctt cagtgtcttc cctgcttatg gttccgtttt
     1261 caaagcactc tattgcaaat gctattgtgt tgccggcgga tattgtgtcc atgcccgccc
     1321 tgttcatgat ttcatttatg tagtatatgg aatccagact ctcgtttaga agcaaggcac
     1381 caaaagagtt taccgtttca tactcaggct tgtgggtggt gtggaatttt cctcctgtca
     1441 catcggatat gttacatacc cctccacagc cgatcacgca ggaatagcag tgatatttcc
     1501 tttcctcccg ttcaattatc ctatcgggat tcatgtttct gtaggatgaa aaatggtagt
     1561 caaggattga acctccccag ttcttgacag gggcgtctcc gcttggaagt ccaaatgtat
     1621 tggagaatat tgttccccac tttttcatca ttggtatgct catcattcca tcctgtggca
     1681 tcaggccacc tgccttttcc atcaatctcc cggccatggg cagcatgctt ccctttacaa
     1741 ttccaggcaa acttacattc ttaaccttgt cgctgtactc cttggtaatc tttagtatct
     1801 cttccctgtc atgggccttg atttttccgg ttcctgcaag taccacagcc tttaggtgct
     1861 ttgagcccat gacagcacca agccctgacc ttccccccat tctacccttg tcatttgaaa
     1921 ttcctgcaat cagggacctt ttttccccag cctgaccgat aacagcgacg gctgccttcc
     1981 tttctccacc gtgcatctcc agaagcatct cctctgcctc cacagcatcc cttccccaca
     2041 gaaggctggc atccatcagt ctcggtccat catcatccac ataaaggtat acaggattct
     2101 cagaaatccc tctgaagaat attccatcat agccacttct ctttattgca gaggaaagat
     2161 tgcccccaca gtttgaatcc ccccagcctc cggtgattgg agatttccct acagctaacc
     2221 accttcctgt aacaacactg ccggttcccg tgagaagccc gcttacaaag cccagcatgt
     2281 tatcctctcc tagagggtcc gcaccctgtg gaatgttcct gtagagataa tatgctccaa
     2341 gtccgacccc tgaaaggaag ctctcgtaaa cttcatcagc taccacttca tccctaaatt
     2401 ctcctgttga taggtcaaca aacaaaacct ttcctatgta acccttcata tcgtcactcc
     2461 ttcctcaccc ctattgttac ccccaataaa ggttcttccc accaaaaaag gaaagccttc
     2521 tagactttcc ttcttaatga cttattgttt tttctcacgc tccttgtagt ccttttcaat
     2581 cctatccttc cagtcatcat caatgacctt cctggttctc ttcatgctta cagtatcact
     2641 taggacttgg tagttcagtc ttgtcccctc cccatcagga ttgtagtttg ttgccctatc
     2701 cctgctgatt ccaaattgtt ttgcagtttc tgccgcatcg atattggccc caaggaaaag
     2761 aaattcccaa ccatattccg ccttctgata attgatcatc ttccttaccc tgtcatatcc
     2821 gtactccctg ctggcattct ccatcccgtc tgttatgatt acaaacatga cgttttcagc
     2881 ctgctcatca ggagcggtat gcctttgtgc atttattatc ttctgaattg tcctgcccac
     2941 tgcatccaga agtgccgtgg ttccccgtac ataatactcc ctttctgtga ttggcttgat
     3001 ccccttgatg tttatcctgt cgtgaagaag ttcatagtcg tcatcaaaca gcacagttgt
     3061 cacaagggcc tctccccttt catccttctg cttttccaag agggaattga a
//
LOCUS       DASRQF010000149         8540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_880217, whole genome shotgun sequence.
ACCESSION   DASRQF010000149 DASRQF010000000
VERSION     DASRQF010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 8540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8540
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880217"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VFD79_04095"
     CDS             <1..545
                     /locus_tag="VFD79_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004817217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZH93261.1"
                     /translation="PCEVCKGKRYNRETLQVKYKGKTISDVLDMTVEEGVEFFKNLPK
                     IRRKLETLYDVGLGYIKIGQPSTELSGGEAQKVKLATELSKRATGKTVYILDEPTTGL
                     HSADIHKLVKVLNQLVEGDNTVIIIEHNLDVIKTADHIIDLGPEGGDKGGYVVATGTP
                     EEVSQCQKSYTGQFLKKILS"
     gene            559..1143
                     /locus_tag="VFD79_04100"
     CDS             559..1143
                     /locus_tag="VFD79_04100"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HZH93262.1"
                     /translation="MCGRYALDADIDELIERYKAIIAEREISGSKEIYPTNTQAIVVD
                     RDQREIRSAVWGFSPHYAKRPLINARGETVHQKPTFSKAFFTSRCIVPATSFFEWEKS
                     EDGKIKRRISLDGQRIFSMAGLVSMFKDKEGKTFEAYTIITISANDQMDHIHDRMPVI
                     LKPEDEDAWLNTSNANLDQLKGLLVPTGQKLNIM"
     gene            1368..2168
                     /locus_tag="VFD79_04105"
     CDS             1368..2168
                     /locus_tag="VFD79_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZH93263.1"
                     /translation="MKKIIALMLTMVMMVGLLAGCTSGASEATSKVDQIKSEGKIVLG
                     TAADYPPYEFHKEIDGQDQIVGFDIEIAKAIAEELGVELEIVDMKFEGLLPALVTNDI
                     DFIIAGMVANEERKESVDFSIPYYQAQQRMIVKVEDAETLKTPADFDGLKIGAQKSTI
                     QEAIALDKFASAEYVGLSKITDLVLELKNGKVDGVILVEPVAKAYVNQNDDLFLAEEI
                     LGQEDGVAVAINKGNDDLVQEIDKVLQNLMDEGMIEKFIADATALAEE"
     gene            2253..2924
                     /locus_tag="VFD79_04110"
     CDS             2253..2924
                     /locus_tag="VFD79_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005953908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HZH93264.1"
                     /translation="MGIFDLEFIAQYYKYFLRGTGVTLSVSFFGVVFGTMVGVVLSLM
                     KLSNNKIAKGLSTAYIEVVRGTPLLVQLFIVYYGFPMFLPFNMSRIALGTVAVLLNSA
                     AYVAEIIRAGILSIDRGQMEAARSLGMTHGMAMRFIIIPQAFKNILPALGNEFIVLIK
                     ESAIISVIGIHDIMYNADTLRGITYRPFIPLFYAAIIYFIITFTLSKLLGIFERRLRA
                     GDQYN"
     gene            2908..3633
                     /locus_tag="VFD79_04115"
     CDS             2908..3633
                     /locus_tag="VFD79_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HZH93265.1"
                     /translation="MINIINLNKKFGDLHVLKDINQTIQEGEVVVVIGPSGSGKSTFL
                     RCLNLLEEPTSGEIILEGVSITDKNNDVNRQREKMGMVFQQFNLFPHKTVLENITLAP
                     IQVKGLTKEEADRKAMSLLKRIGLEEKANTYPNQLSGGQKQRIAIIRALAMEPDVMLF
                     DEPTSALDPEMVGEVLEVMKELARDGMTMVVVTHEMGFAKEVGSRVIFMDEGRIMEEG
                     SPKDLFENPRNPRTQDFLRKILV"
     gene            3752..4837
                     /gene="eutH"
                     /locus_tag="VFD79_04120"
     CDS             3752..4837
                     /gene="eutH"
                     /locus_tag="VFD79_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HZH93266.1"
                     /translation="MLYVMLGFSLLGGIDKLLNNRFGLGIKFDEGFKAMGSLALTIIG
                     IYSLSPVIARGILPLLGPLSTWMNVDPSVFVSSILATDLGAFNTSVEIARDPAIGVFN
                     GAILASTLGATISFTVPVAINLITEDDFQYFAKGILAGIVTVPLGMVVSGLMLKIGIM
                     KILWNLLPVMVFSLIIAYGLIKSQEKTMFLFRWLGKLILGISTFGLILGILDFSLGIV
                     LIEGLISLEEATILVLSISIVLSGAYPMLYFLSRGLNRVLRQVTERYDIDDFSILGLF
                     SSLASCLPMLGVYHEMNWKGKILNAAFAVSGAYVFGGQLGYVTSVAPQAVNAFITGKL
                     AAGVTSIFVALYLIRMEIKGEGIKIEY"
     gene            4827..6614
                     /locus_tag="VFD79_04125"
     CDS             4827..6614
                     /locus_tag="VFD79_04125"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HZH93267.1"
                     /translation="MNINEKLKELRKLMRDEGIDVYIEPSADPHQSEYLADHFKSRAY
                     ITGFTGSAGTALVTDKEAILWTDGRYFIQAENQIKDNEFKLYKMNTPGYPSLQEWLEE
                     NVKYGARIGLNGRIFSQGAVEKIEDGLKDKKVSIHHEDDLIGKIWDDRPHLPIRDAFM
                     LGEEYSGKSTAEKIKEIRLEMEKKNATHFLLASLDDIAWLYNIRGWDVDNNPVLISYA
                     MVTFDYAMLFVDERKIPESVKTDLYDSGVVLADYDSIDQHLGDLPKDSTIILQKDRVN
                     TSLFHAIPKNCRIIDTDNMTTIMKGIKNPVEIKNQEEAYLKDCTALTGFFHWLHKEMD
                     QREITEVEASERLLKFRMEQEGFLQPSFGTIAAYGPNGAMMHYSPDEASCALLQKKGL
                     FLVDSGGQYYNGTTDITRTVALGELTDLEKRDFTLVLKGHINLLSSRFLHGTSGHVLD
                     VLARQPLWKEGVDYKSGTGHGVGYLLNVHEGPHRIATAPNNVAMEPGMVVTVEPGVYR
                     EGMHGIRTENVAVVRKDMENESGTFLSFQLLSYCYIDRECIVPEMLTDDEKLWLDQYH
                     MNVYEKLKNRLDQEVGEWLKDKTRPIDEK"
     gene            6642..8510
                     /gene="uvrC"
                     /locus_tag="VFD79_04130"
     CDS             6642..8510
                     /gene="uvrC"
                     /locus_tag="VFD79_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009431229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HZH93268.1"
                     /translation="MTNFEEQLKNLPDSPGVYLMKDKAGEIIYVGKAISLKKRVRQYF
                     QSSANHSLKVSSMVKQIRDFEYIMVENEVEALVLESNLIKKNRPKYNILLRDDKQYPY
                     IKVTVGERYPRVLKTRQVEKDGSRYFGPYPSASAVNEAIEVFHRIFPIRNCRLDLNRK
                     DARFRPCLNFYIGRCLGPCLGNVDEDAYNRMIQEILDFLTRKDESIIRDMEKSMKEAA
                     EGMDYEKAANYRDQLQALQTLHEKQMMDSASNVEQDVIAMARGVEEVVVQVFFIREGK
                     IVGREHFIMEDSYVEERGEILSSFIKQFYPGSTFIPKEILVEEEIPDLDAIQQWLGTI
                     KGSRVSLQTPKRGDKLQLVRMVKKNALEMLDKYGDKFLRKHRENLKALDQVQHLLDLE
                     ERPDRIEAYDISNISGVGSVGSMIVYEKGERKKSDYRRFRIRTRTSPDDYGSMEEVLK
                     RRFTRGLKENENVERREGAPVGFSLFPDIIMVDGGKGHVNKASAVLKELGVDIPVCGL
                     VKDRFHKTRGIIYDNREIIPDKDSLAFKLIYGIQEEAHRFAISYHRSLRSKDLFRSEL
                     DGIPLVGEKRRVALLKHFGSISEIKKASLEDLAEVDGMNIKAAQNVYEHFRIKEEK"
BASE COUNT         2587 a         1674 c         2246 g         2033 t
ORIGIN      
        1 ttccctgtga ggtctgcaag ggcaagaggt acaacaggga gacccttcag gtcaagtaca
       61 agggaaagac catttcagat gtactagaca tgactgttga ggaaggtgtt gagttcttca
      121 agaaccttcc aaagatcagg agaaagctgg aaaccctgta tgatgtgggg cttgggtata
      181 taaaaattgg ccagccgtca acggagcttt caggtggtga ggcccaaaaa gtcaagctgg
      241 caacggagtt gtccaaaagg gccaccggaa agacagtcta tattcttgac gagcctacaa
      301 caggccttca ttccgctgat atacacaagt tggtgaaggt gctgaatcag cttgtagagg
      361 gtgacaatac agtcatcata atcgagcaca acctggatgt aataaagacg gcagaccata
      421 tcatagacct tgggcctgaa ggaggggaca agggaggcta tgtggttgca accggtacgc
      481 cagaggaggt aagtcagtgt cagaaatcct acactggcca attcctcaag aagatattgt
      541 cttgagagga gggagagtat gtgtggtaga tatgccctgg atgcggacat agatgaattg
      601 atcgaaaggt acaaggcgat tattgcagaa cgtgaaatca gtggctcaaa agagatttac
      661 cccaccaata cccaggccat agttgttgac cgggaccaga gggaaatcag gtcggcagtt
      721 tggggatttt caccccatta cgccaagaga ccactgataa atgcaagagg ggagactgtt
      781 caccagaagc ccaccttcag caaggccttc ttcaccagca ggtgcatagt tcccgccaca
      841 agtttttttg agtgggagaa gtcagaggat gggaaaatca aacgaaggat ttcccttgat
      901 ggacagagga tattctctat ggcagggctg gtaagcatgt tcaaggacaa ggaaggaaag
      961 acatttgaag cctatacaat aatcacaatt tctgcaaatg atcagatgga ccacatccac
     1021 gacaggatgc cggtcatact gaagccggag gatgaggatg cctggctgaa caccagcaat
     1081 gcaaacctgg atcaattgaa gggattgctg gttcctacag ggcaaaaact aaatattatg
     1141 taaacttaat gaagtttttc ttgcattatc agaattaaaa gaatataata tgtttcatat
     1201 ttaaaaacaa aattcaataa aggaggtctt aatcttgatg gataaattta atcagatcaa
     1261 gagccaacgg aagtttggtt cactaaacat ggacaagact tccaaagggc taaggagttc
     1321 ctcactatta taatccaaga aaactaaaat aataggagga ataaaaaatg aaaaaaatta
     1381 tagcacttat gttgacaatg gtaatgatgg taggtttatt ggcaggctgc acctcaggag
     1441 cctcagaggc cacttccaag gtggaccaga tcaagtcaga gggtaagatt gtacttggta
     1501 ctgctgcaga ctatccaccc tatgagttcc acaaggaaat tgatggtcag gaccagattg
     1561 taggctttga cattgaaatt gccaaggcaa tagctgaaga gcttggagtg gaactggaaa
     1621 tagtggacat gaaattcgaa ggacttttac ctgcacttgt gactaacgac atcgatttca
     1681 taatcgctgg aatggttgca aacgaggaga gaaaggaatc cgttgacttt tcaataccat
     1741 attatcaggc acagcaaaga atgatcgtta aggtagagga tgctgaaacc ctaaagactc
     1801 cggcagattt tgacggattg aagatagggg ctcagaagtc aaccattcag gaagcaatag
     1861 ctcttgataa gtttgcctct gcagaatacg taggtttgag caagattacc gacctggttc
     1921 ttgaactgaa gaacggaaag gtcgacggag tcatactggt tgaaccggtt gccaaggcct
     1981 atgtaaatca aaatgatgac cttttcctgg cagaagagat cctaggtcag gaggatggag
     2041 ttgctgtagc aatcaacaaa ggaaacgacg atttggttca ggagattgac aaggtgcttc
     2101 aaaatctaat ggacgaaggc atgattgaga aatttatcgc tgatgcaacg gcattggcag
     2161 aagaataaga tattccccgg caaaagagag ttcgctttct tttgcctttt ctattaggaa
     2221 aaagcgggcg tatcccataa ggaggaaatg aattgggaat cttcgatctg gaatttatag
     2281 cgcaatacta caaatatttt ttaaggggta ccggagtaac ccttagcgtc agcttctttg
     2341 gagtggtctt cggaaccatg gtcggcgtgg tcctaagcct gatgaagctt tcaaacaaca
     2401 agattgcaaa gggactgtcc actgcctaca tagaggtggt acgtggaacc ccactgcttg
     2461 tgcagctttt tattgtctac tacggattcc caatgtttct gccatttaat atgagcagaa
     2521 tcgccctggg aacagtagct gtcctcttaa acagtgcagc ttatgttgca gaaataataa
     2581 gggcgggaat cctatccatt gacaggggcc agatggaggc ggccagatcc ctgggaatga
     2641 cccatggcat ggcaatgcgg ttcatcatca tcccacaggc tttcaagaac atccttcctg
     2701 ccctgggcaa tgagttcata gtcctgatca aggaatcggc aatcatatct gtaattggaa
     2761 tacatgacat aatgtacaat gcagacacat taagggggat aacctacaga ccatttatcc
     2821 cattgttcta cgcggcaata atatatttca tcataacttt taccctatcc aaactcttgg
     2881 gtattttcga aaggaggtta cgtgccggtg atcaatataa ttaatctaaa caagaaattc
     2941 ggtgatctcc acgttttaaa ggatatcaac cagaccatcc aggaaggtga ggttgttgtg
     3001 gtgatcggac ccagtgggtc tggtaagagt accttcctaa ggtgtctgaa ccttcttgag
     3061 gagccgacaa gtggagagat catccttgag ggagtatcca taacagacaa gaacaacgac
     3121 gtcaacaggc aaagggaaaa gatgggcatg gtattccagc agttcaacct cttcccacac
     3181 aagaccgtac ttgaaaacat aaccttggca cccatccagg tcaagggact taccaaggag
     3241 gaggcggaca ggaaggccat gagtcttctt aagaggatag gcctggagga gaaggcaaac
     3301 acatacccca atcagctttc cggaggtcag aagcagagga tagcaataat aagggccctg
     3361 gcaatggagc cagatgtaat gttgtttgac gaacctacaa gtgccttgga cccggaaatg
     3421 gtaggcgagg tgctggaggt tatgaaggag ctggcaaggg atggcatgac catggtggtt
     3481 gtaacccatg aaatgggctt tgccaaggag gttggctcca gggtcatttt catggatgaa
     3541 ggaagaatca tggaggaggg atctcccaag gacctttttg aaaatcccag gaatccaagg
     3601 acacaggatt ttctaagaaa gattcttgta tagctgaaac aaagtataag tcataaatga
     3661 gcgtctctct tgaggcgctt ttttgttcgt tgtgattggg gttcacattg tagtataatt
     3721 agactgatga tgacatcatg ggagggattt tatgctttat gttatgctgg gtttttcact
     3781 tctggggggg attgacaagc ttctcaacaa caggtttgga cttggaatca aattcgatga
     3841 gggattcaag gccatgggga gcctggcctt gactataata gggatatatt ccctctcgcc
     3901 agttatagct agggggatac tgcccctatt ggggcccttg tccacgtgga tgaatgtgga
     3961 cccttcagtt tttgtaagca gtatacttgc aactgacctt ggggcattca ataccagtgt
     4021 tgaaattgca agagacccgg caatcggggt tttcaacggt gccatactgg cctcgaccct
     4081 gggagccaca atctccttca cagtacctgt ggcaataaac ctaataacag aggatgactt
     4141 tcaatatttt gccaagggga tccttgcagg tattgttaca gttcccttgg gcatggtggt
     4201 ttctggcctg atgctgaaaa tagggataat gaagattttg tggaaccttc ttcctgttat
     4261 ggtgttttct ctgatcattg cctatggtct tataaaatcc caggaaaaga ccatgttcct
     4321 cttcaggtgg ctgggcaaac taatcctagg aataagcaca tttggtctga ttcttggtat
     4381 actggacttc agtctgggta tagtcctgat tgaaggactg atttcacttg aggaggcaac
     4441 aattctggtt ctttccatca gtattgttct gtctggagca tatccaatgc tctacttcct
     4501 atccaggggt ctcaacaggg tcctgaggca ggtaacggaa agatatgaca tagatgattt
     4561 ttcaatactt ggactttttt catctctggc aagctgcctt ccaatgctgg gtgtctacca
     4621 tgaaatgaat tggaagggta agatcctcaa tgcggccttt gcagtaagcg gggcatatgt
     4681 atttggaggt cagctgggct atgtcacatc tgtggcaccc caggcagtga atgcctttat
     4741 cacaggcaag cttgcagcag gggtgaccag tatttttgtt gcactatacc taatacgaat
     4801 ggaaataaaa ggggagggaa taaagattga atattaatga gaagttaaag gaactaagga
     4861 agctaatgag ggatgaggga atagacgttt acatcgaacc gtccgcagac ccccaccagt
     4921 cggaatacct ggcagaccat ttcaagtcca gggcctatat aaccggcttt acagggtctg
     4981 cgggaactgc ccttgttact gacaaggagg ccatcctatg gacagatgga agatatttca
     5041 tacaggctga aaaccagatt aaggacaatg agttcaagct ttacaaaatg aacacgcccg
     5101 gttatccgtc cctccaggaa tggcttgagg aaaatgttaa gtatggggcc agaattggcc
     5161 ttaacgggag gatattttcc cagggagcag tggagaagat agaggatggc ctgaaggata
     5221 agaaggtcag tatccatcat gaggacgact tgataggaaa gatatgggat gacaggcccc
     5281 acctgcccat tagggatgca ttcatgctgg gggaggagta ctcagggaag tcaaccgctg
     5341 aaaagatcaa ggaaatcaga ctggaaatgg aaaagaaaaa cgcaactcac ttcctattgg
     5401 caagcctgga cgacatagcc tggctttaca acataagagg ctgggatgtt gacaacaatc
     5461 ctgttctgat ttcatatgca atggttacct ttgattacgc catgcttttt gtggatgaaa
     5521 gaaagattcc ggagtcggtt aagactgacc tgtacgattc aggagtggta ttggcggact
     5581 acgacagtat tgaccagcac cttggagacc tacccaagga ttcaaccatt atcctccaaa
     5641 aggacagggt aaatacaagc ctcttccatg caattccaaa gaactgcagg ataattgata
     5701 cagacaatat gacaactatc atgaagggaa taaagaatcc tgtggagata aaaaaccaag
     5761 aggaggctta tctcaaggat tgtacagcct tgacaggctt tttccactgg cttcacaagg
     5821 aaatggacca gagggaaatt actgaggtgg aggcttctga aaggcttttg aagttccgga
     5881 tggagcagga gggcttcctt cagccaagct ttgggacaat agcagcctac ggccccaatg
     5941 gggccatgat gcattattct ccagatgaag ccagctgtgc cttgctccaa aagaagggac
     6001 tctttctggt ggattcggga ggtcagtact acaatggaac aactgacata accagaaccg
     6061 tagccttggg agagcttaca gacctggaga agagagattt cacacttgtg ttgaagggac
     6121 atataaacct tctatcctca agattcctcc acgggacatc tggacatgta ctggatgtat
     6181 tggccaggca gcctctttgg aaggagggtg ttgactacaa gtcaggaacg ggacatggag
     6241 tgggatacct tctaaatgta cacgagggac cccataggat agccactgca cccaataatg
     6301 ttgcaatgga gccgggaatg gtggttactg tcgaacctgg agtttacagg gaagggatgc
     6361 acggtatcag gacagaaaac gtagcggtgg taaggaagga catggaaaat gaatctggaa
     6421 ccttcctgag cttccagctg ctgtcttact gctatatcga ccgtgaatgc atagttcctg
     6481 agatgctgac cgatgatgag aagctatggc tggaccagta ccacatgaat gtgtatgaaa
     6541 agcttaagaa tagattggac caggaagtgg gagaatggtt aaaggataag accaggccaa
     6601 tagatgaaaa gtaacaggtt tcatggaaat agggtgattt tgtgacaaac tttgaagaac
     6661 agctgaaaaa tctgccggat tccccgggag tatatcttat gaaggacaag gccggagaga
     6721 ttatctatgt ggggaaggcc atctccctta agaaaagggt caggcagtac ttccaatcct
     6781 cggcaaacca ttcactcaag gtatcatcaa tggtcaagca aataagggac tttgagtata
     6841 taatggtgga gaacgaggtg gaagcccttg tgctggagtc aaacctcatc aagaaaaaca
     6901 gacccaagta caacatactc ctgagggacg acaagcagta tccatatata aaggtgacag
     6961 tgggagaaag gtatccaaga gtccttaaga ccagacaggt ggagaaggat gggtcaaggt
     7021 atttcggacc ataccccagt gcatctgctg tcaatgaggc aatagaggta tttcacagga
     7081 tatttccaat caggaactgc aggcttgacc tcaataggaa ggatgcaaga ttcaggccct
     7141 gcctaaactt ctatataggc aggtgtctgg gaccctgttt gggcaatgtg gatgaggatg
     7201 catacaacag aatgatacag gaaatccttg attttctcac cagaaaggac gagagcatta
     7261 tccgagatat ggaaaagtcc atgaaggagg ctgccgaagg tatggattat gaaaaggcag
     7321 ccaattacag ggaccagctt caagccctgc agacactcca tgagaagcag atgatggatt
     7381 ccgcatccaa tgtggagcag gatgtgattg ccatggccag gggtgtggaa gaggtggttg
     7441 ttcaggtctt cttcatacgt gaaggcaaga ttgttggaag ggagcacttt ataatggagg
     7501 attcctatgt tgaggaaagg ggagaaatcc tcagctcctt catcaagcag ttctatcccg
     7561 gatctacctt tattccaaag gagatacttg ttgaggagga aatacctgac ctggatgcca
     7621 tccaacaatg gcttggaaca ataaagggat caagggtaag tcttcagact cccaagaggg
     7681 gagacaagct tcagctggtc cggatggtca agaaaaatgc ccttgaaatg ctggacaagt
     7741 acggggataa gttcctccgc aagcacaggg aaaatctcaa ggctctggac caggtccagc
     7801 acctcttgga ccttgaggaa aggcctgaca ggatagaggc ttatgacatc tccaacatca
     7861 gtggtgtagg gtcggtggga tccatgatcg tctatgaaaa aggggaaagg aagaaatccg
     7921 attacaggcg tttccgaatc agaacaagga caagccctga cgactatgga agcatggagg
     7981 aagtattgaa gcggaggttt accaggggtc taaaggaaaa cgagaatgtt gagaggagag
     8041 agggagcacc tgttggattc tcactttttc cagatatcat tatggtagac ggtggaaagg
     8101 gacatgttaa caaggcaagt gccgtgctga aggagctggg cgtcgatata cccgtttgcg
     8161 gacttgtaaa ggacaggttt cacaaaaccc gtggaataat ttatgacaac agggagataa
     8221 tccccgacaa ggacagcctt gccttcaagc tgatatatgg aatacaggag gaggcccaca
     8281 ggtttgccat aagctaccac aggagcctaa gaagcaagga ccttttccgg tcggaattgg
     8341 atgggatacc ccttgtagga gagaagagaa gggttgccct cctgaagcac tttggaagca
     8401 tcagtgaaat caagaaggcc agccttgaag accttgcaga agtggatggt atgaacatca
     8461 aggcagccca gaatgtatac gagcatttcc gaataaagga ggaaaagtaa tgcaaaaaaa
     8521 gctatatttg tcagatacag
//
LOCUS       DASRQF010000150         2955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_498415, whole genome shotgun sequence.
ACCESSION   DASRQF010000150 DASRQF010000000
VERSION     DASRQF010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498415"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VFD79_04135"
     CDS             complement(<1..426)
                     /locus_tag="VFD79_04135"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HZH93269.1"
                     /translation="MKLNKIIEEKRKQMEKAKKKETAKNVAVGTAIGTALGAAAGLLF
                     APKSGKETREEISTKGKEVAETVKVKVEDQLDTTKEHLEKVKTDVEKGWVELKEKAKK
                     EKNEAEEAMDEAAEKAEDVVDEIKKGAEEVKEVAKDTKED"
     gene            643..2418
                     /locus_tag="VFD79_04140"
     CDS             643..2418
                     /locus_tag="VFD79_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZH93270.1"
                     /translation="MKSFLTIKEYFLEHKKYYIMGVLWLIVIDGVQLIVPQILRRLTD
                     DLQFGNLDRGRLIFYAFLIVATGLFIGIGRYFWRIYLIGTSREIEYKLRNKLFSHLLS
                     LSPNYFNTHKTGDLMAHATNDINAVRMALGPGTIMIVDSAFLLMFSLIMMVRTTNLSL
                     TAAAVFTLPLIMFLVTRFGKIIYKKFKVVQEAFSDLSDITQESFSGIRVIKSFVQEKL
                     VSENFKRVNQNNFNKNMDLVKVHGGFHPLLQLISSISYLVIIFYGGRQVINGSISLGD
                     FVAFNSYLGIIIWPTRALGMVINVLQRGAASLDRLNVIFDEKSDIVQKESPVKLSPLK
                     GEIEFRDVAFAYPNSNHEALTKVSFKIPAGKTLAITGRTGSGKSTIANLILRLFDTST
                     GEILVDGHNIRDVSLDDLRGNIGFVPQDNFLFSQTIEDNIALAYDRKPMESEIIEASR
                     IAQLHDNIIEFPDKFKTVIGERGVTLSGGQKQRLSIARAIVKKPSVLILDDSLSAVDT
                     QTEEKILSGIRNFSRDITTILISHRISTVKGADEIIFLDDGTILEKGTHLDLLEKKGE
                     YYDLYQRQLLEDQVRSEEDENGQTS"
     gene            2402..>2955
                     /locus_tag="VFD79_04145"
     CDS             2402..>2955
                     /locus_tag="VFD79_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93271.1"
                     /translation="MDRLHEEEVLGKAYDWKLMKRLLKYARPYWALLTVSILLLIVIT
                     GLELLNPYLVKIAIDDHISGYNRPLVQVPLEAGPIEDGIVVDNHIYVLKEEYENTGNY
                     PVRSIVQRAEGYYLVDADDPSSPGELLSQEDYGAFRSRDIEGLDRVAKLFAAVILLTF
                     LLSYLQIYILNYTSQKIIFNIREDL"
BASE COUNT          852 a          619 c          606 g          878 t
ORIGIN      
        1 atcttcctta gtgtctttgg ctacttcctt aacttcctct gcaccttttt ttatctcgtc
       61 aactacgtct tctgcctttt cagcagcttc atccattgct tcttcagctt cattcttttc
      121 cttcttagcc ttttccttca actcaaccca gcctttttca acatcagtct ttaccttttc
      181 caggtgttct ttggtagtat caagttggtc ctcaacctta acctttacag tttctgccac
      241 ttccttaccc ttggttgaaa tttcttccct agtttccttg cctgactttg gagcaaataa
      301 aagtcctgcc gctgcaccta atgcggtacc tatagctgta cctacagcta cattcttggc
      361 agtctccttt ttcttggcct tttccatttg ttttctcttt tcctcaatga tcttattcaa
      421 tttcatcatt atatctcccc tttgtaattt ttctatacac acactttacc cccaaaatat
      481 ctgtaataat caaacttaga caaattattt gctgaacagg cttatttggg ttcattttaa
      541 cctgcttatg aaaataacca tctaatttgg aaaaaggagg aaagtttata taaaacttgg
      601 tataatgtta tttgacactc tggtcaaaca aggaggataa tgatgaaatc atttttgact
      661 ataaaggaat attttcttga acacaagaaa tattacatca tgggagtatt atggctaata
      721 gtaatagatg gagtgcaact gattgttcca caaatactta gaaggcttac agatgacctt
      781 cagtttggaa acctggaccg tggcaggttg attttttatg cctttcttat tgttgccaca
      841 ggtctgttca taggaatagg cagatacttc tggaggattt atctgattgg aacttccagg
      901 gagattgagt ataagctaag gaacaaattg ttttcgcatt tgttgagcct atcccccaat
      961 tatttcaaca cccataagac aggagacctt atggcacatg ccacaaatga catcaatgcc
     1021 gttagaatgg cccttgggcc tggtaccata atgatagtgg attcagcatt cctattgatg
     1081 ttctccctta tcatgatggt tagaaccaca aatctaagtt tgacggcagc agccgtattc
     1141 acccttccct tgattatgtt tttggtgaca cgattcggga agataatcta caaaaagttt
     1201 aaggtggtcc aggaagcctt ttcagacctt tcggacatta cacaggaaag cttttcaggc
     1261 ataagggtga ttaaatcatt cgtacaggaa aagctggttt ctgagaactt taaaagggtc
     1321 aaccagaaca acttcaataa aaacatggat ctggtgaagg tgcacggtgg cttccacccc
     1381 cttctccagc tcataagctc aataagttat ctggttataa tattctatgg aggacggcag
     1441 gtaatcaatg gctcaatttc attgggggat tttgttgcct tcaactccta ccttggaatc
     1501 ataatctggc caacaagagc ccttgggatg gttataaatg tccttcagcg tggtgctgct
     1561 tcattggaca ggctaaatgt catatttgat gaaaagtctg atattgtcca aaaggaatcg
     1621 cctgtgaagc tatcccccct aaagggtgag attgaattta gggatgtagc ctttgcctac
     1681 ccaaactcaa accatgaggc actgacaaaa gtcagcttta aaattccagc cggtaaaact
     1741 cttgccataa ccggaagaac cggcagtggg aaaagcacca ttgcaaatct cattctgaga
     1801 ttgttcgata caagcacagg agaaatactt gtggatggcc ataacataag ggatgtgtca
     1861 cttgatgacc ttaggggtaa cattggattt gtcccccagg acaatttcct cttttcacag
     1921 acaatagagg ataacatagc cttggcatat gacaggaaac caatggagtc agaaattatt
     1981 gaagccagta ggattgccca gcttcacgat aatatcattg aatttccgga taagttcaaa
     2041 actgtcattg gcgaacgtgg agtaacccta tccggtggtc aaaagcagag gctcagcatt
     2101 gcaagagcaa ttgtaaagaa gccatctgtt ttgatccttg atgatagcct gtctgcagtt
     2161 gatactcaaa cggaggaaaa gatactcagc ggtatcagga atttttcaag agatataaca
     2221 actatcctga tcagtcacag gatatctaca gtcaagggtg ctgatgaaat catattcctt
     2281 gatgacggta ctatcctgga aaagggcact cacctggatc tccttgaaaa gaaaggtgaa
     2341 tactacgact tgtaccaaag acaattattg gaagaccagg tcagaagtga ggaggatgaa
     2401 aatggacaga cttcatgaag aagaagtgct tggaaaagcc tatgactgga aattgatgaa
     2461 gagactcttg aagtatgcaa gaccctactg ggctcttctt actgtttcca tcctcctgct
     2521 aatagtaata acaggtctgg aacttttgaa tccatacctt gtgaaaatag ccatagacga
     2581 ccatatcagt ggatataaca ggcctctggt tcaggtccca ttggaggcag gccccattga
     2641 ggatggtatt gttgtagaca atcacatcta tgtgttgaag gaagagtatg aaaacacagg
     2701 taactatcct gtgagaagca ttgtacagag agccgagggc tactacctgg tggatgctga
     2761 tgatccaagc tctcctgggg aactcctgtc acaggaggat tatggtgcct tcagaagcag
     2821 ggacatcgag ggactggaca gggtagcgaa gctgtttgct gcagttattc ttctgacctt
     2881 ccttctaagc tacctgcaga tatacatcct aaactacaca agtcagaaga tcatcttcaa
     2941 tataagggaa gacct
//
LOCUS       DASRQF010000151         3266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1497995, whole genome shotgun sequence.
ACCESSION   DASRQF010000151 DASRQF010000000
VERSION     DASRQF010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497995"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /gene="serS"
                     /locus_tag="VFD79_04150"
     CDS             <1..1071
                     /gene="serS"
                     /locus_tag="VFD79_04150"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004831860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HZH93272.1"
                     /translation="EPVFAQMKELSDKVKEFDGQVKDVDDELKTLLLQIPNTPHNTVV
                     EGESDEDNVEVRKHGTPKEFNFEPKAHWDIGTELDVLDFERASKISGARFSVFKGAGA
                     RLERAIIGFMLDLHTGEHGYTEMSTPFMVNRDSMVGTGQLPKFEEDMFHVPSKDFFLV
                     PTAEVPLTNLLRDEIITEDQLPIYYTAYTPCFRQEAGSAGRDTRGLIRNHQFDKVEMV
                     KFSHPDKSYEELETLTNNAEEVLKRLGIPYRVVMLSTGDLGFSSAKTYDVEVWMPSYN
                     RYVEISSCSNFEDFQSRRANLRFRREDSGKVEYLHTLNGSGLAVGRTFAAILENFQEE
                     DGTVTIPEALRKYMGGMEKLEK"
     gene            1270..2277
                     /locus_tag="VFD79_04155"
     CDS             1270..2277
                     /locus_tag="VFD79_04155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HZH93273.1"
                     /translation="MNKKTLSLWLVFILTLALMVGCSPAAEEPAGEEPGGEAPAENVF
                     ISVATGGTGGTYYPLGGAIAKIFNENIAGVTANAQSTGASAENVNLVAQGEAEVAFVQ
                     NDVTYYAWTGTETFDGQDPITNIRGMAMLYPEVIQVIATADSGIATIEDLAGMRVAVG
                     APGSGTEVNARQILAEYGMTYSDLGKADYLSFNEAADQLKNKQVDAAFVTGALPTSAV
                     TEVTQTADIVVVAMDSARIASLSAEYPFYTEVAIPGDTYNGMTGDVTSAAVMAMLIVP
                     EDLSEDLVYNMTKSMFEQRQVIIDTHARGNDIKLETALAGMPIDLHAGAQKYYDEQGI
                     E"
     gene            2258..2815
                     /locus_tag="VFD79_04160"
     CDS             2258..2815
                     /locus_tag="VFD79_04160"
                     /inference="COORDINATES: protein motif:HMM:NF020479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HZH93274.1"
                     /translation="MSKASSRKEFNGKGTDSISTFPLLKRILLVVVALLAIAMMLYPI
                     EVIKASDARSGEYLGSWWPMEDKSFTIKYIHSVERTPVWEMYTIEEGEIVLKETIFQS
                     YGAGLPATSPYDFDIVEDGFRLYNIDQKMTNLVYRTGAVRANHELMVGERTYPFVEFS
                     EPTEEVKFETQKTPILLYLAKEGFK"
     gene            2812..>3266
                     /locus_tag="VFD79_04165"
     CDS             2812..>3266
                     /locus_tag="VFD79_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter permease"
                     /protein_id="HZH93275.1"
                     /translation="MNDDKRIDNENLEEEIKTEEVVDDQVAVEKEAESIEDVLGEDDN
                     LDVDSLMESYDKEASKLRKLKGAFSTIVMVIAIAMSSFHLYTAGFGTLLSYRQRSLHI
                     IFAFIIGLFLYPARKKSSREKPAIFDFLLMGVAIVVFGYIFLFPEQMAL"
BASE COUNT         1026 a          632 c          806 g          802 t
ORIGIN      
        1 gagcccgtat ttgcacaaat gaaggaactt tcagacaagg tcaaggaatt tgacggtcag
       61 gtgaaggatg tcgatgatga gctgaagacc ctgcttcttc agattccaaa caccccccac
      121 aataccgttg ttgagggtga gtccgacgag gacaatgttg aagttagaaa gcacggaacc
      181 ccaaaggagt tcaattttga gccaaaagcc cactgggaca ttggaactga gctggacgtc
      241 cttgattttg aaagagcatc aaagatttcc ggagccagat tcagcgtttt caagggtgct
      301 ggtgcaagac ttgaaagggc cataataggc tttatgctgg accttcatac aggtgagcac
      361 ggctacactg aaatgtccac cccatttatg gtcaacaggg acagcatggt tggaacaggt
      421 cagctgccaa aatttgagga ggacatgttc catgttccca gcaaggactt cttccttgtt
      481 cctacagcag aggtaccact tacaaatctc ttgagggatg agatcattac agaggaccag
      541 ttgccgatat attacaccgc ctacacccct tgcttcagac aggaggcggg atctgcaggc
      601 agggacacga gaggacttat tagaaaccac cagtttgaca aggttgagat ggtgaagttt
      661 tcacatcctg acaagtcata cgaggagctt gaaaccctca ccaacaacgc tgaggaggtt
      721 ctgaagaggc tgggaattcc ttacagggtt gtaatgctaa gcacagggga ccttggcttc
      781 tcatctgcaa agacctatga tgttgaggta tggatgccaa gctacaacag gtatgttgaa
      841 atttcaagct gcagtaactt cgaggacttc cagtcaagaa gagccaacct gagattcaga
      901 cgggaggata gcggcaaggt agaatacctc cacactctaa atggcagcgg ccttgcagtt
      961 ggaaggacat tcgcagcaat tcttgaaaac ttccaggagg aagatggaac cgtaactatt
     1021 cctgaagccc ttagaaagta catgggaggc atggaaaagc tggagaaata gaaaatcatg
     1081 gtccgaagag cactcttcgg accattccac ctttagaagg caaaaacgtg acaaacatca
     1141 aacttaagaa ctatttaaca aaattttagt ggattatcac ttaaaattga gttataatga
     1201 atttataagt tgtccaaata gtcagaaagg gacggcttga aaggtagaaa atcaaaaagg
     1261 gggatgaata tgaacaaaaa gacattatca ttatggttgg tatttatact gactctagca
     1321 cttatggtag gttgctcgcc agcagctgag gagccagcag gagaagagcc tggtggagaa
     1381 gctccagcag agaacgtatt tatatctgta gcaactggtg gaaccggcgg tacttactat
     1441 ccacttggtg gagcaattgc caagatattc aacgagaata tcgcaggcgt aactgcaaat
     1501 gctcaatcaa ctggtgcatc agctgagaac gttaaccttg tagctcaagg tgaagctgaa
     1561 gtcgcttttg tacagaacga cgtaacttac tatgcatgga caggaactga aacctttgat
     1621 ggacaagacc caataacaaa catcagagga atggcaatgc tttatcctga ggttattcag
     1681 gttatagcaa cagctgactc aggaatcgca acaatagagg accttgctgg aatgagagtt
     1741 gcagttggag ctcctggatc aggaactgag gttaatgcta gacagattct tgcagagtat
     1801 ggaatgactt atagtgacct tggaaaagct gactaccttt catttaacga agctgctgac
     1861 cagcttaaga acaagcaggt tgacgctgca ttcgtaacag gcgcactacc aacatcagct
     1921 gtaactgagg taactcagac tgcagatatc gttgtagttg caatggactc agctagaatc
     1981 gcttctctat cagcagagta cccattctac actgaagtag ctatacctgg agatacctac
     2041 aatggtatga ctggagatgt tacatcagca gcagtaatgg cgatgcttat agttccagaa
     2101 gatttgagcg aggacttggt ttacaacatg actaagagca tgttcgagca aagacaggta
     2161 atcatagata cacatgcaag aggaaatgac attaagcttg aaactgcatt ggctggtatg
     2221 ccaatagacc ttcatgcagg tgctcagaag tactacgatg agcaaggcat cgagtaggaa
     2281 agaattcaat ggaaaaggta ctgatagtat cagtaccttt cccttattaa agaggatttt
     2341 gcttgtggtc gtggctcttc tggcaattgc gatgatgctt tatccaattg aggttataaa
     2401 ggcctctgat gcaagaagtg gagagtatct gggttcatgg tggccgatgg aggacaagtc
     2461 cttcacaatc aagtatatac attccgtaga acggacacct gtatgggaga tgtataccat
     2521 tgaagaggga gagatcgtac ttaaggaaac catttttcag tcctacggcg ctggacttcc
     2581 ggcaacaagt ccctatgact ttgacattgt agaggatggg tttcgcctgt acaacattga
     2641 ccaaaaaatg acaaacctcg tttacagaac tggagcagta agggcaaatc acgaactgat
     2701 ggtaggggaa aggacatacc ctttcgttga attttcagag cctaccgagg aggtaaagtt
     2761 tgaaacccaa aaaactccaa tattactata tttggcaaag gaggggttta agtgaatgac
     2821 gataagagaa tagacaatga gaatcttgaa gaagaaataa agactgaaga agttgttgat
     2881 gaccaggtag cagttgaaaa agaggcagaa tccatcgagg atgttctagg tgaagatgat
     2941 aatcttgatg ttgattcact gatggagtcc tatgacaagg aagcatcaaa actaaggaag
     3001 ctaaagggag cattctcgac gattgttatg gtaatagcaa tagccatgtc atccttccac
     3061 ctgtatactg caggctttgg aacattgcta tcctacagac agagatcgct acatattata
     3121 tttgcattta taattggatt attcctttat cccgcaagaa agaagtcaag cagggaaaaa
     3181 cctgcaatat ttgactttct gttgatgggg gttgcaattg tggtgtttgg atacatattc
     3241 ctcttcccgg agcagatggc tcttaa
//
LOCUS       DASRQF010000152         3034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_940169, whole genome shotgun sequence.
ACCESSION   DASRQF010000152 DASRQF010000000
VERSION     DASRQF010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940169"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..523
                     /locus_tag="VFD79_04170"
     CDS             122..523
                     /locus_tag="VFD79_04170"
                     /inference="COORDINATES: protein motif:HMM:NF021462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HZH93276.1"
                     /translation="MSLIKMYGISFTVFFAIDLIWLGLIAKNLYREHLGYIMSPSVNW
                     VAAIGFYIFYIAGLIFFAIAPAIERDSLLYAAFVGAFFGFITYATYDMTNLATLKDWP
                     VFITVVDIIWGTVLCGLTASVSFIILKAIGI"
     gene            677..790
                     /locus_tag="VFD79_04175"
     CDS             677..790
                     /locus_tag="VFD79_04175"
                     /inference="COORDINATES: protein motif:HMM:NF019948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93277.1"
                     /translation="MGILRSYAIKYKQEYILSQHIWEPFPEEMLDISDTGK"
     gene            complement(808..2340)
                     /locus_tag="VFD79_04180"
     CDS             complement(808..2340)
                     /locus_tag="VFD79_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93278.1"
                     /translation="MLLNFTEKLDFLMNLTGTSNSAMAKQTSLDPSFISRLRSGSRKP
                     SRNENYISKMAAVLSKRCREDYQMQGLLTRLQAENISYSRETISLDVAVEEWLRSIPA
                     EQKSKVGSFIRQLDEFSFKKDPSDHGEGGEAYLDESRQDVYHGIHGKRVAALRFLTDI
                     LNSDRKHTLYLYSDEDLTWLLDDPHFTMQWAKIFMQVLQKGNKVKIVHTITRSLDEMF
                     SAIKEWMPFYLTGNIEPYYYPRTRDINFRRTIFIAPDLCAVSSSSIGRNSESNLNFYL
                     TEKETLQSILREFDDFLKLCRPLMKIFNISTSESLMDTVNEFEEESGNCILRSQAVSS
                     ITIPLFALEKFLKTHVGENSDKLFNFHKQRQRALVEILKTNKFVELIQLPDISQLVER
                     EIPIGFTVFSGTPVFYDPYTFKAQLENIVSLMKHYPNYEVVIQDNVKDELTIYAKENI
                     GLLIMKYTKLIISFAVNEPYMTQAFWDYLRMASPQVSNTATTRKETMKKIDDYLKKLK
                     DV"
     gene            2484..2780
                     /locus_tag="VFD79_04185"
     CDS             2484..2780
                     /locus_tag="VFD79_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93279.1"
                     /translation="MSYTIKLKSGFFKTLKYTMMVEDDQLILRPDLAEGVDLIAINNE
                     DVLELGIIRYPNGRREFEIITTGGYYFGCISPAVDLDVLVGVLKKSFGRRFSIE"
BASE COUNT          873 a          537 c          665 g          959 t
ORIGIN      
        1 agaagtttat gttcgactga aattcagggt tttaaagcta ttggaagggg tagatatacc
       61 aatattcata acggattaaa gtgtgaatgg aattgtaaca actacagaac gagctgaaga
      121 gatgagtcta ataaaaatgt atggaatatc ctttactgtc ttctttgcaa tagacttgat
      181 atggttggga ctcattgcaa agaatcttta cagggagcat ttgggttata ttatgagccc
      241 cagcgtaaac tgggtggctg caatcggttt ttacatcttt tatatagctg gacttatctt
      301 ttttgcaatc gcaccggcaa ttgaaaggga tagtcttctc tatgctgcat ttgtaggtgc
      361 attttttggg tttataacct atgcaaccta tgatatgaca aaccttgcaa cattgaagga
      421 ttggccagtc tttataacag ttgttgacat catttgggga acggttcttt gtggactgac
      481 tgcctcagta agctttataa ttcttaaggc aataggaatc tgatgttgga ggggatctaa
      541 atgacaatta gcaggctaaa tggggaggca ctttattacg ctttcttgtc aggtgcaaac
      601 gaggtaataa agcagaagta tgaactgaag aagtcttgaa cgattcaaga aaaaaaaggc
      661 ccacctgggc gtacctgtgg gaatactaag aagctatgct atcaagtaca agcaggaata
      721 cattctttca cagcatatat gggagccatt cccggaggag atgctggata taagtgatac
      781 tggcaaatga aaaaggctca aggatgtcta gacatccttg agctttttta gataatcatc
      841 gatttttttc atggtttctt tcctggtagt ggctgtattt gagacttgtg gtgaagccat
      901 tctcaaataa tcccagaagg cttgagtcat gtagggctcg ttgaccgcaa agcttatgat
      961 gagctttgtg tacttcatta taagcaaacc aatgttttcc ttagcgtata tggtaagttc
     1021 atcctttaca ttatcctgga tcaccacctc gtaatttgga taatgcttca taaggcttac
     1081 aatgttttca agctgagcct tgaaggtata tgggtcataa aataccggtg ttccagagaa
     1141 aacagtaaag cctattggta tctctctctc aacaagctgg gaaatgtcag gaagctgaat
     1201 gagctccaca aacttgttgg ttttaaggat ttcaaccagt gcccgctgcc tttgcttgtg
     1261 aaagttaaag agtttgtcac tattttctcc cacatgggtt ttgaggaatt tctccaatgc
     1321 gaataggggt atggtgattg atgagacagc ctgggatctt agaatacagt tcccgctttc
     1381 ctcctcgaac tcattgacag tatccattaa ggactcgctg gtggaaatgt tgaatatttt
     1441 cattaagggc ctgcatagct tcaggaaatc atcaaactcc cttaggattg actgaagggt
     1501 ttccttttct gtcaggtaga agtttagatt gctttctgaa tttctgccga ttgaactgga
     1561 cgaaactgca cataggtctg gagcgataaa aatggttctt ctaaaattaa tatctctggt
     1621 tctgggataa taatagggct caatattgcc agtcaagtag aatggcatcc actcctttat
     1681 tgcggaaaac atctcatcaa gggacctggt aatggtgtga actattttaa ccttgttccc
     1741 cttttgaagt acctgcataa aaatctttgc ccactgcatc gtaaaatgag ggtcatccag
     1801 aagccatgtc aggtcttcat cactatacag atacagagta tgctttctgt cggagtttag
     1861 aatatcagtc agaaacctaa gggctgcaac acgtttgcca tgaattccat gatagacatc
     1921 ctgccttgat tcatccaaat aggcctctcc accttctcca tggtctgaag gatccttttt
     1981 aaaactgaat tcatccagct gccttataaa gcttccaacc ttgctttttt gttccgctgg
     2041 aatgcttctt agccattcct caacagctac atccagactg atggtttctc tggagtagga
     2101 gatgttttct gcttgtagcc tggtcaatag tccctgcatc tgataatcct ccctacatct
     2161 cttggaaagg acggcagcca tctttgagat atagttttca ttccgtgaag gcttccttga
     2221 gcctgaacgg agacggctta taaaggaagg gtccagggaa gtctgcttgg ccattgcact
     2281 attgctggta cctgttaaat tcattagaaa atcaagcttt tcagtgaagt tcaatagcat
     2341 cacctcttta gttgattata gcaagtggtc tattccttgg caagattttg tcacgattat
     2401 tgcccgtaat tgacaatgcc aagagtgatt atgtgaatca taggaaggct tttatggtat
     2461 aaataaacaa agaggtgtgt gtcatgagtt atactattaa gttgaaatca ggatttttca
     2521 agacgttgaa atacactatg atggttgagg atgatcagtt gatattaagg cctgatctgg
     2581 ctgaaggggt agacctaata gcgataaata atgaggatgt ccttgagctt ggtatcatca
     2641 ggtatcccaa tggtaggagg gagtttgaaa taataacgac cggaggatat tacttcggtt
     2701 gtatttcgcc tgcagttgat ttggacgtcc ttgtaggagt acttaaaaag agttttggca
     2761 gaagatttag cattgagtga agacctctgg aactaaataa tccagaggtc tttgctatca
     2821 gtattaatat tcttgtgtgc tagagttatt ccagtagtaa ttgttagttg agaatcttgc
     2881 ataattgaaa attctaacct aaaatgacaa aacataacta tatatagcgc ttttgttgtt
     2941 tgtgtcatac tatatgtagc gggcaagtaa tgaataatat aaatatgcaa tttcctcagt
     3001 tgagtaaatt gcataattta aaattgttga aata
//
LOCUS       DASRQF010000153         6397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_877636, whole genome shotgun sequence.
ACCESSION   DASRQF010000153 DASRQF010000000
VERSION     DASRQF010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6397
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877636"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VFD79_04190"
     CDS             <1..861
                     /locus_tag="VFD79_04190"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HZH93280.1"
                     /translation="TIPDGTYEGVSDAGMHAGLKVAVTVEGGVITNVEVVAHDETPGI
                     SDPAIEEVPVAIVEANSTIVDVVSGATYTSDAIMEAARFALMAGASNAGADQGPVTYV
                     DGTYEGSSDAGMHPGLKVTVTVEGGVITNVEVVAHDETPGISDPAIEEVPAAIVAANS
                     PSVDVVSGATYTSDAIMEAVGLALEGAVAEAGAAPAVAYTDGTYEGSSDAGMYPGLKV
                     SVTVEGGVITNVEVVAHDETPGISDPAIEEVPAAIVAANSPSVDVVSGATYTSDAIME
                     AVGLALEGAQ"
     gene            complement(928..1209)
                     /locus_tag="VFD79_04195"
     CDS             complement(928..1209)
                     /locus_tag="VFD79_04195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HZH93281.1"
                     /translation="MSTHVITVKYEIRPERMDEFIQLIKENSEKSLAEPGCLNYEASM
                     AGNKVFLYEKYKSEDDFKYHTTTEHFANYGEKTKDLVINKEITMYKGLK"
     gene            complement(1237..1479)
                     /locus_tag="VFD79_04200"
     CDS             complement(1237..1479)
                     /locus_tag="VFD79_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HZH93282.1"
                     /translation="MKVNLDNLVSITEANQNFSKVARMVDKKGSAVIMKNNKPRYILI
                     EYSQISEYETAEDQDIDIVAERLVTKYMDAFKELEK"
     gene            1795..3759
                     /locus_tag="VFD79_04205"
     CDS             1795..3759
                     /locus_tag="VFD79_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZH93283.1"
                     /translation="MKKILLITMGENTGRALSKQLNDLYGNMLKVTCHLLEDLPVPES
                     GYDLVVFSSKYVKDRASRSFSGNISHIVAHRVVNHKNIRDLISLEKGMEVLFVNDGFE
                     SATEAIEQLQDLGLDHIKYYPYFPGIQKYPRLSTIVTPGESQLCPYGASDSIDIGSRI
                     LGLSTIFEINNILFGNQISNHAIAESYLKDIVEISKSIDKAGKETGEIKDILEKITNS
                     LEFGVAFIDNQGIVRSINSRFEKMLQIKSMDLINKKASDIDGINRIVESGEGSYDTRV
                     GNKNIHLEMKEVTYQNRVGQLVKAYYDQEGSSRKALYGNNRNLHDFDDYYTLDPHVLG
                     MVEKARKYASTEATILIHGENGTGKEILAQAIHKNSPRRNKSFVPINIAAISSNLVES
                     ELFGYEEGTFTGALKGGREGLFQVADGGTLFIDEIGDTPLDIQAKLLRALEEKRVRRV
                     GGSREEAVDVRIIAATNRNLLEMVGEGTFRQDLFFRLNILPLETIPLVNRPSDVDMLL
                     KMFLGSYMKSRSVENLNDIFTSEALNILRRYRWIGNVRELKNLTEYLSHIYTGEKIRR
                     EDLHGYILDHTKEKGQEEPLEDWILKVLGSYGQVPVGRRRLLSQASENGYDLGEGQLR
                     ALLDDLKERGLVETVRNKGMILTEKARKNK"
     gene            3924..5384
                     /gene="nhaC"
                     /locus_tag="VFD79_04210"
     CDS             3924..5384
                     /gene="nhaC"
                     /locus_tag="VFD79_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004815606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HZH93284.1"
                     /translation="MEKKEQVKRKPTLLQGLIPILFMVIALTVGVGVLKLRTEPIIIL
                     SAFVAGIIAWRLGYTWKEMQDGVIEKIARALPATLILWSVGLLIGSWMFSGTVPMIIY
                     YGVDIVNPQYLLVTAFIISAVLSTVTGTSWGSAGTIGVAIMGIAGGLGVSLPATAGAV
                     VAGAYFGDKISPLSDTTNLAPIAAGSELYEHIKHMFWTTIPAATVSLIVYFIVGMNSA
                     GGASTPDSVLVLQDQLSQMFNWNLILFLPVLFIIAGSLLKWPTIPTMLGTSLFSVLLG
                     ILVQGYTLKDGFVSLIQGFNVSMTGFAGEVAPAVNTLINRGGVVSVTTTTVLIFCAMG
                     FAGIISVSGMLDVVLEALLRRVKTTAGIILSTIASCATVAFVTGSSYLSILIPGELFK
                     DAYKMRNLHAKNLSRTLEDSGTVLVPLIPWSAAGAYMTATLGVSTIEYLPWAVLNYMG
                     IVFAILLAVTGIGITKLDGGKKEDKGALGNAVNQEN"
     gene            5362..>6397
                     /gene="iadA"
                     /locus_tag="VFD79_04215"
     CDS             5362..>6397
                     /gene="iadA"
                     /locus_tag="VFD79_04215"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HZH93285.1"
                     /translation="MLLIKKINLFSPEALGTKDVMIINEKIIEIGDNLEPISDRMIVI
                     DGTGKIMVPGLIDGHVHLTGGGGEGSFRTRVPEITLSKLIEGGITTVLRLLGTDSTTR
                     NVENLVAKSKALNEEGITSYALTGSYGYPSVTLTDSVKKDITYVNEIIGVKIAISDHR
                     APSTSKEELARLASDARVAGMLSGKAGVVVTHMGEGKGRLKPIRDVLENWDLPMKTFI
                     PTYVNRNDDLLKEALDYARQGGTIDLTCGIYDERTPGRVVAEALKAGVPGENITISSD
                     GYGSWSKYDEKGNLLKIGVSSVSSLYNELRETVKAELISLEKALPFFTTNVARALGLD
                     RSKGRIAKDME"
BASE COUNT         1929 a         1338 c         1606 g         1524 t
ORIGIN      
        1 acaatacctg atggaaccta cgaaggtgta agtgatgcag gaatgcacgc aggcctaaag
       61 gttgcagtaa cagttgaagg tggagtgata acaaatgtag aggttgttgc ccatgacgag
      121 acacctggaa tatcagatcc ggcaatagaa gaagttcctg tggcaatcgt tgaagcaaac
      181 agcacaattg ttgatgtagt atcaggagca acctacactt cggatgcaat aatggaagct
      241 gctagatttg ccctaatggc gggagcatca aacgctggag cagatcaggg tccagtaacc
      301 tatgttgacg gaacctacga gggaagcagc gatgcaggaa tgcatcctgg cctaaaggtt
      361 acagtaacag ttgaaggtgg agtaataaca aatgtagagg ttgttgccca cgatgagaca
      421 ccaggaatat cagaccctgc aattgaggaa gtgccggcgg caatcgtagc ggcaaactca
      481 ccaagtgttg atgttgtatc aggagcaacc tacacatcag atgcaataat ggaggcagta
      541 ggcctggctc ttgagggagc ggttgcagaa gctggggcag caccggcagt tgcatatact
      601 gacggaacct acgaaggaag cagcgatgca ggaatgtatc ctggcctaaa ggtttcagtc
      661 acagttgaag gtggagtaat aacaaatgta gaggttgttg cccacgacga gacaccagga
      721 atatcagacc ctgcaattga ggaagtgcca gcggcaatcg tagcggcaaa ctcaccaagt
      781 gttgatgttg tatcaggagc aacctacaca tcagatgcaa taatggaggc agtaggcctt
      841 gcacttgagg gagctcagta atagattaat aagatacaat aaaacggagg ctatatcaca
      901 gttaatgtgg tatagcctct tttaggttta cttaagaccc ttgtacatgg tgatttcctt
      961 attgattaca aggtccttgg tcttttcgcc gtagttggca aaatgctcgg ttgtggtgtg
     1021 gtacttgaag tcgtcctcgg acttgtactt ctcatagagg aaaaccttgt ttccagccat
     1081 ggaagcctcg tagttgagac agccaggctc agccaggctc ttctcggaat tctccttgat
     1141 aagctggatg aactcgtcca tcctctccgg cctgatttca tacttaacgg taataacatg
     1201 tgtactcatt gtaaaacccc tttctttatt aaaatattat ttttctaatt ctttaaacgc
     1261 atccatatat ttcgtgacta gcctttcagc aactatatca atatcttgat cttcagccgt
     1321 ctcgtactct gaaatttgac tgtattcaat caatatataa cgaggtttgt tgtttttcat
     1381 aattacagct gatccctttt tatcaaccat tcttgcaact tttgaaaaat tttgattagc
     1441 ttcagttatt gaaaccaggt tatcaagatt taccttcatt ttactcaact cctttctaat
     1501 ctcattatac ccaaaatata ggataaaaac aacctaaaac aataactcct atataagcgt
     1561 tagctagaaa agtatactcg taatccgaca atctttgtca tcttaagcct gcgaaggatc
     1621 ctggtccctt caataaaaca agattcttcg actacgctca gaatgacaac gcaattttca
     1681 gctgtgaatt gtctttccca aattgtcatt ccctcaaaat actgatagaa tatacccatc
     1741 aagaaaagaa aagccacaag aataaagtct gttgagacca ataggagaat gcttatgaag
     1801 aagatattat tgataaccat gggtgagaac acgggaaggg cacttagcaa acagctaaat
     1861 gacctttatg ggaatatgct aaaggtaacc tgccacctac ttgaagacct gccagtgcca
     1921 gagtcaggct atgaccttgt ggtgttcagc agcaagtacg tgaaggacag ggcaagcagg
     1981 tcctttagcg gaaatatcag ccacatagtt gcccacaggg tcgttaacca caagaacata
     2041 agagacctta taagccttga aaagggcatg gaggtacttt ttgtaaacga cggcttcgaa
     2101 tcggcaacag aggccatcga acagctgcag gacctggggc ttgaccacat caagtactac
     2161 ccatactttc ctgggatcca gaagtatccc agactttcaa ccattgtgac accaggtgaa
     2221 agtcagctgt gtccatacgg ggcctccgac tccatagata taggttcaag gatactggga
     2281 ctgtcaacga tttttgaaat caacaacatt ctctttggta atcagatttc aaaccatgcc
     2341 atagctgaaa gctacctcaa ggacatagtg gagatctcaa aatcaataga caaggccggc
     2401 aaggaaaccg gcgaaatcaa ggacatactt gagaagatca caaacagcct tgagtttggg
     2461 gttgccttta tcgacaacca gggaatcgtc cgtagtatca acagcagatt tgaaaagatg
     2521 cttcagatca aaagcatgga cctcatcaac aagaaggcat cggatatcga tgggataaac
     2581 aggattgtgg aatcaggcga gggcagttat gataccaggg ttggaaacaa gaacatccac
     2641 ctggagatga aggaggtgac ctaccagaac agggtgggtc agctggtgaa ggcatactat
     2701 gaccaggagg ggtcctcaag gaaagcccta tacgggaaca acaggaacct gcacgatttt
     2761 gacgattact acacccttga tcctcatgtc ctgggcatgg ttgaaaaggc cagaaaatat
     2821 gcctccactg aggctaccat ccttatccat ggagaaaatg gaacggggaa ggaaatcctt
     2881 gcccaggcca tccacaagaa ctccccaaga aggaacaagt cctttgtccc cataaacatc
     2941 gcagccatat cctccaacct tgtggagagt gagctgttcg gctacgagga gggaaccttc
     3001 acaggagccc taaagggtgg tcgggaaggt ctgttccagg tcgctgatgg agggaccctc
     3061 tttatagatg aaattgggga tacccccttg gacatacagg ccaagctcct gagggccctg
     3121 gaggagaaac gggtaagaag ggttggtggc agcagggaag aagccgttga tgtgaggata
     3181 atcgcagcca ccaacaggaa cctcttggaa atggtagggg aggggacctt caggcaggac
     3241 ctcttcttca ggctcaacat cctacctctg gaaaccatac cccttgtgaa taggccctct
     3301 gatgttgaca tgctgcttaa gatgttcctt ggttcataca tgaaaagcag gagtgtggaa
     3361 aacctcaatg atattttcac cagtgaagcc ttgaacatac tcaggcgcta caggtggata
     3421 gggaatgtaa gggagctaaa aaacctcacc gagtacctga gccatatata cacaggggaa
     3481 aagatcagaa gggaggacct gcatgggtat atactggacc ataccaagga aaaaggacaa
     3541 gaagagcccc ttgaggactg gatattaaag gtgcttggat cttatggcca agtgccagtt
     3601 ggcaggagaa gattgctgag ccaggcatca gaaaacggct atgacctggg agagggtcag
     3661 cttagagccc tcctggatga ccttaaggaa aggggcctgg tggaaaccgt ccgcaacaag
     3721 ggtatgatcc taacggaaaa agcaagaaaa aacaaataac gcaggataaa acccgccaac
     3781 agttggatag catccagatg caggcgggtt tgttttgagg gacaatttta aaaagtcagt
     3841 aaatatgccg gctctccttt gagacaaatt tggcatggat attgcaataa taaaatcata
     3901 taaaatcaaa ggagagtgat tgtatggaga agaaggagca ggttaagagg aagccaacgt
     3961 tgttgcaggg tcttatacca atattattta tggttattgc gttgacggtt ggggttggag
     4021 tattgaagct aagaactgag cccatcatta tactttctgc atttgttgca gggataatcg
     4081 cctggagact tggctacacc tggaaggaaa tgcaggatgg ggtaatcgag aaaatagcaa
     4141 gggcacttcc ggctacactt atactgtgga gtgtgggact acttattgga tcatggatgt
     4201 tctcaggaac tgtaccaatg atcatctact acggtgttga catagtaaat cctcagtacc
     4261 tcctggtgac agcctttata atttcagcag tcctgtcaac agtaacagga acatcatggg
     4321 gttcagcagg aacaatcggg gttgcaatca tgggaatcgc aggtggactt ggagtatcac
     4381 tgccggcaac agcaggagcc gtggttgcag gagcatactt tggtgacaag atatctccac
     4441 tttcagatac gacaaacctt gcacccatcg cagctggctc agaactttat gagcatatca
     4501 agcacatgtt ctggacgaca ataccagcgg ctacagtttc acttattgtg tatttcatag
     4561 taggaatgaa ttcagcggga ggagcaagca ctccagattc tgttctggtt ctccaggacc
     4621 agttgagcca gatgttcaat tggaacctta tcctcttcct gccggttctt ttcattattg
     4681 cagggtccct tctcaagtgg ccaacaattc caaccatgct tggaacaagc cttttctcag
     4741 tattgttggg aatattagtc cagggttata ccctgaagga cggttttgtg tcactgatcc
     4801 agggctttaa tgtaagcatg acaggctttg caggagaggt tgctccggct gttaacaccc
     4861 ttatcaacag gggtggggtc gtatccgtaa ccacaacaac agtcctgata ttctgcgcaa
     4921 tgggttttgc cggcataata agtgtatccg gtatgcttga tgttgtactt gaggcactac
     4981 ttagaagggt taagacaaca gcgggcataa tcctctcaac aatcgcatca tgtgcaactg
     5041 tggcgtttgt aacgggaagc tcataccttt caatccttat accgggagaa ctgttcaagg
     5101 atgcatacaa gatgagaaac ctgcatgcaa agaacctttc acgtacactt gaagactcag
     5161 gtactgtgct tgtaccattg ataccatggt cagctgcagg agcctatatg actgccacgc
     5221 ttggggtatc gacaatagag tatcttccat gggcagttct taactacatg ggtatagtat
     5281 ttgcaatact ccttgctgtt acaggcatag gaataacaaa gcttgacgga ggtaagaagg
     5341 aagacaaggg ggcattaggt aatgctgtta atcaagaaaa ttaatctgtt ctcaccggag
     5401 gcactgggaa caaaggatgt aatgataata aatgaaaaaa tcattgaaat tggcgacaac
     5461 ctggagccaa tatcagacag gatgattgta atcgacggta ctggaaagat aatggtaccg
     5521 ggactgatcg atggtcatgt tcacctgaca gggggaggag gagaagggag cttcaggaca
     5581 agggttcctg agataaccct ttcaaagctg attgaaggcg gaataacaac tgtcctaaga
     5641 ctcttgggta cagactcaac aacaaggaat gtggaaaacc ttgtggcaaa gtcaaaggcc
     5701 cttaacgagg aaggtatcac aagctatgcc cttaccggct cctatggcta cccatcggtt
     5761 acattgacgg attcagtgaa gaaggacatc acatatgtca atgagataat tggcgtaaag
     5821 atagcaatat cagaccacag ggctccaagc acctccaagg aggagctggc aaggcttgca
     5881 tctgatgcaa gggttgcagg catgctctca ggcaaggccg gggttgtggt tacccatatg
     5941 ggagaaggaa agggcagact aaagccaatc agggatgtcc ttgaaaactg ggaccttcca
     6001 atgaagacct ttataccaac ctatgtaaac aggaatgatg acctgctgaa ggaggctctg
     6061 gattatgcaa gacagggagg aacaattgac ctgacctgcg gaatctatga cgaaaggact
     6121 cctggaaggg tagttgccga ggcccttaag gcaggggttc cgggtgaaaa catcaccata
     6181 agctcagatg gctacggcag ctggagcaag tacgacgaga agggaaacct tctaaagatt
     6241 ggggtatcct ctgtttcaag cctttacaat gagctgaggg agacagtgaa ggctgaactc
     6301 ataagccttg agaaggcact gccattcttc acaacaaatg ttgccagggc actcggactg
     6361 gacaggtcaa aggggaggat tgccaaggat atggaag
//
LOCUS       DASRQF010000154         3431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_432373, whole genome shotgun sequence.
ACCESSION   DASRQF010000154 DASRQF010000000
VERSION     DASRQF010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432373"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..746
                     /locus_tag="VFD79_04220"
     CDS             51..746
                     /locus_tag="VFD79_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZH93286.1"
                     /translation="MENIVEIRGLTKRYDRKEALRSIELSIEKGKVVGILGPNGSGKT
                     TLIKLLMGLLHPNKGEIKIDGESPGIHTKSVISYLPDRNSLYSWMTVKDALQMYQDFY
                     QDFDGKKAEDLLQFMKLERTMRVSDMSKGMKEKLNLALVLSRRAKVYILDEPIAGVDP
                     VARDQILDAIIDNYKEDSAMIITTHLVRDMENIFDQVVFLGDGEIILSGEAESLREEK
                     NMQIDDLYKEIFG"
     gene            758..1600
                     /locus_tag="VFD79_04225"
     CDS             758..1600
                     /locus_tag="VFD79_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93287.1"
                     /translation="MFKYAKYELRGTMKFMIGALILALGASTGIQLFLIRTAGGLGGS
                     GMTGIVEYAATLMLALVFVVLGSIVGGMVYLISSYRRELYEDRGYLTFSLPLSGGQLL
                     GSKLIVALFWGTVMGISIILYNLILAQILFGHNLLGEMWKITGQIDNFGFLFTGSIVT
                     SLMESAVTLLLVYFAITVSRVSIKSRKIGSLWFILFIVLTSLVTYIQELIANHLPMYL
                     DLSRMAIIGQKEVYIMGLDSQFGGTLSIQGVVSIPGLVFQILLIAGAFIATSYMLEKK
                     IDIV"
     gene            1669..2919
                     /locus_tag="VFD79_04230"
     CDS             1669..2919
                     /locus_tag="VFD79_04230"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HZH93288.1"
                     /translation="MSRRLIATLIVFFILLGAFVFTVIREEHRNMDQLGKEYPELSQD
                     AYDLRSDALKLWAIRLPLGFILPLLLLFTGFAQRVSLFAGERFGVVISGLFVGLAIFG
                     IIYLVNLPLSFYSSFILRHRYGLTDQTLFRWLELSLKGFLVNKGILALFIWVPYMIIQ
                     WRPDGWWLPMGILAVPVAIFMVFISPIVIDPVFNTYSQLPQGQLRENMETLLKRGEIG
                     AAEIYVVDKSRDTKTMNAYMTGILSGKRIVFWDTTLNNLEDQEVLSIAAHEMGHYIRG
                     HIWKGILGGSVGIFLVLYLMNLSANWILEASGGVFGFRNIYNYASLPLLLLLLNLFLF
                     LGNPVTNGVSRYFEREADLYQISLAQDRESAVTAMTKLYEKSLGLPRPSELYKFWYSS
                     HPPIEERIEFYRTEDFLTFGGEES"
     gene            2916..3404
                     /locus_tag="VFD79_04235"
     CDS             2916..3404
                     /locus_tag="VFD79_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93289.1"
                     /translation="MIFIPVLLAAALLGVFAYKIGHRRNGNKGRSWAITLMSIIFGIT
                     VLVAGILLVVVLDPDVQVVGDRIQVSGLYKRDIHLEDVEDVSLKKELPVIQDQVNGLS
                     IGNTLRGTFRIEGGEISTMFVQKNTPPFIHIRTGERLFVINFRDSQETEDLYGEIMEE
                     LE"
BASE COUNT          960 a          639 c          895 g          937 t
ORIGIN      
        1 agggctgata ttccagggat aattgaaaag atcatagtgg aggggaaata atggagaata
       61 ttgttgaaat aagaggactg acaaagagat atgacaggaa agaggcttta agaagcatcg
      121 aactttcaat agagaaggga aaggtagtgg gtattctcgg tcccaatgga agcgggaaga
      181 ccacccttat aaagctgctt atgggtttgc ttcatccaaa caagggagaa ataaagattg
      241 atggggaaag tcctggaatc catacaaagt cggtcatatc atacctgccc gatagaaatt
      301 cactctacag ctggatgacc gtaaaggacg cacttcagat gtatcaggac ttttaccagg
      361 attttgacgg taagaaggcg gaagaccttc ttcagttcat gaagcttgag aggacaatga
      421 gggtcagtga catgtcaaaa ggcatgaagg agaagctgaa cctggcactg gttctttcaa
      481 gaagggcaaa ggtatacatc ctggacgagc caatagctgg ggtggatcct gttgccaggg
      541 accagatact ggatgcaata atagataact acaaagagga cagcgcaatg ataataacca
      601 cccatcttgt gagggacatg gagaacattt tcgaccaggt ggtttttctt ggggatgggg
      661 agataatcct ttcaggggaa gcagagagtc taagggaaga aaagaatatg cagattgatg
      721 atctgtacaa ggaaattttt ggctaggggg aatatgaatg tttaagtatg caaaatacga
      781 actaagggga acaatgaagt tcatgattgg agcattaata ctggccttgg gtgcctcaac
      841 aggcatccag ctgtttttaa taaggacagc cggcggcttg ggtggttcag ggatgacagg
      901 aatagtcgaa tatgcagcta cactaatgct ggctcttgtt tttgttgtat tgggatcaat
      961 agtcgggggc atggtatacc tgatatcctc ttacaggaga gagctttacg aagacagggg
     1021 ctacctgacc ttttcactgc cccttagcgg aggccagctt cttggatcaa agcttattgt
     1081 ggccctgttc tggggaactg taatgggtat atccataatc ctttacaacc tgatattggc
     1141 ccagattctt tttgggcata atcttctagg ggagatgtgg aagataactg gacagattga
     1201 caacttcggt tttctattta ctgggtctat agtcacctcc ctgatggaga gcgctgtaac
     1261 ccttcttctg gtctactttg caataaccgt aagcagggtt agcataaaaa gcagaaagat
     1321 aggaagtctt tggtttatac ttttcatcgt tctcaccagt cttgtaacct atatccagga
     1381 attaattgcg aaccacctgc caatgtatct tgacctttca aggatggcta taatcggcca
     1441 gaaggaagtc tatatcatgg gtctagattc acagtttgga gggacactgt ccatacaggg
     1501 agtggtttcc atacctggac ttgttttcca gattcttctt atagcaggag catttattgc
     1561 aacctcctac atgctggaga aaaaaatcga catagtttaa aagaatagtt gaacacccct
     1621 taatgtggta caataaagtc agaaagagcc atagtcaggg gtgtttttat gagtagaaga
     1681 ttgattgcaa cattaatagt tttttttata ttgctgggag cctttgtatt cacagttata
     1741 agggaagagc ataggaatat ggaccagcta gggaaggagt atccggagct ttctcaggat
     1801 gcctatgacc ttagaagtga tgccttgaag ctttgggcaa taaggctacc ccttggattt
     1861 atcctgccgc ttttgcttct gttcaccggc tttgcccaaa gggtgagcct ctttgcaggt
     1921 gaaaggtttg gtgtggtgat ttctggtctg tttgttggac ttgcaatttt tgggatcatt
     1981 taccttgtaa acctaccctt aagcttctac agctcattta tccttagaca caggtatggt
     2041 ttgacggacc agaccctttt cagatggttg gagctgagtc taaagggatt tttggtaaac
     2101 aaggggattc ttgccctttt catctgggta ccctatatga taatacaatg gaggcctgat
     2161 ggatggtggc ttccaatggg gattctggca gtgcccgtag ccattttcat ggtattcatt
     2221 tcaccaattg tcatagatcc tgtattcaat acctattccc aactgcccca gggtcagctg
     2281 agggagaaca tggagaccct tctcaagaga ggggaaatcg gagctgcaga aatctatgtt
     2341 gttgacaaga gcagggatac aaagacaatg aatgcatata tgaccgggat cctttccgga
     2401 aagaggattg tattctggga caccaccctg aacaatctgg aggatcagga ggtccttagc
     2461 atagcggccc acgagatggg acactatata aggggtcaca tctggaaggg gatccttgga
     2521 ggatcggttg ggatatttct tgttctctat ctgatgaacc tttctgccaa ctggatactg
     2581 gaggcatcgg gaggggtctt tggattcaga aacatttaca actacgcatc cctgccacta
     2641 ctactgcttc ttttgaacct ctttcttttt cttggaaatc ctgttacaaa tggtgtttca
     2701 aggtattttg aaagggaggc agacctttac cagatatcac tggcacagga ccgggaatcg
     2761 gcagttactg ccatgaccaa gctatatgaa aagagccttg ggcttcctcg accatcggaa
     2821 ctgtacaagt tctggtacag ctcacacccg ccaatcgagg agaggataga gttctacagg
     2881 acagaggatt ttctgacctt tggaggtgag gaatcatgat ttttatacct gtacttttag
     2941 cagctgccct tctgggagtt tttgcatata aaattgggca taggagaaat ggaaacaagg
     3001 gaaggtcctg ggcaataacc cttatgtcca ttatatttgg aataaccgtg cttgtggcag
     3061 ggattctcct tgtggtggtt ctggaccctg atgtccaggt ggtgggagac aggattcagg
     3121 taagtggact ttacaagagg gacatccatc ttgaggatgt tgaggatgta agtcttaaga
     3181 aagagcttcc tgtaatccag gaccaggtca atgggctcag catcggaaac accttaaggg
     3241 ggaccttcag gatagaggga ggggagattt cgaccatgtt tgtccagaaa aacaccccgc
     3301 catttatcca tatcaggacg ggggaaaggt tgtttgtgat caatttcagg gactctcagg
     3361 agactgagga cctttatggg gagattatgg aggagttgga gtagaagaaa ttcacaatga
     3421 aaagatttaa g
//
LOCUS       DASRQF010000155        11492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_749008, whole genome shotgun sequence.
ACCESSION   DASRQF010000155 DASRQF010000000
VERSION     DASRQF010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 11492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11492
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749008"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1459
                     /locus_tag="VFD79_04240"
     CDS             <1..1459
                     /locus_tag="VFD79_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZH93290.1"
                     /translation="IREDLFNHLQSLAVRYFDNNPIGRLVTRVTNDTETLNEMYTNVL
                     VNLFKDFFMLAGIMIVMIRLNLQLALVSFALIPVILLISIVFRKYIREVYRISRVQLA
                     KINLSLNENISGMRTIQIFKKERKFFENFDSINRDYLNIQKKEVTYFAIFRPSIEVIR
                     SLGIALLVYYGGGNVVSGSIQFGVLFVFINYLQKFYEPIMDLTEKYNILQSAMASSER
                     IFKILDTDMRIEDPHTPARIEHFKGEIEFRNVWFAYNDEDWVLKNISFKIKRGESVAI
                     VGATGAGKTSIINLLTRFYDIQKGEILLDGVNIRSLDKNTLRQKIGVVLQDVFIFTGN
                     IRDNIRLNKDISDDKVIEIAKHVNAHRFISRLPQGYDEPVMERGSTLSSGERQLMAFA
                     RTLVYDPQILVLDEATSSIDTETELLIQDALHKLVEGRTSIAIAHRLSTIQHSDKIIV
                     LKQGEIKEMGSHQDLLKNKGLYYNLYQLQYKENFQ"
     gene            1554..2354
                     /locus_tag="VFD79_04245"
     CDS             1554..2354
                     /locus_tag="VFD79_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HZH93291.1"
                     /translation="MYDFHIHSNFSLDSKATMESVVKTALEKNLKTICFTDHVDFEAT
                     EDKIDLLFRPLDYFRDINRVKYKYRDRIEILAGVEIGMQPGLEARYDSFIEENGFDFV
                     IMSLHTINRKDIFHDYSSTELSSSEIISRYYDELLYCVRSYDNYDVLGHIDFIDRYID
                     DQDVIKKYDLYMDKIESILRTIIDKGKGIEINTGGMRYRLNVPHPKPSILKLYKDLGG
                     DIITFGSDSHVADHVGHEYRSSEKMLKEMGFKYIYLYRNRKKFPIQIQ"
     gene            complement(2478..2656)
                     /gene="ssrS"
                     /locus_tag="VFD79_04250"
     ncRNA           complement(2478..2656)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFD79_04250"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(2706..3605)
                     /locus_tag="VFD79_04255"
     CDS             complement(2706..3605)
                     /locus_tag="VFD79_04255"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZH93292.1"
                     /translation="MHKEDPNTLVFTNKKAPRDLTSFLREKGLSGRMIRRALINGDIS
                     MGDSTCKGGEIVPMDRQVCIRLADEDSDMKPQKMQLEIIYEDMDLLVLNKEPFTLVHP
                     TPNHPDGTLSNGVASYFLECGLKRKVRILNRLDRDTSGIIIFPKNSFGHQQLAAQMEH
                     DKVVKKYLAIVHGNITKDWGEINLALGKGEDGIRQVVRDDGAEAVTRFKVLERVGEGT
                     LVELELLTGRTHQIRVHLSHMGYPIMGDHLYSHGESHIDRQALHAYGMSFVSPRTDEM
                     ISLHAEMPEDMKNLLNFLKSSKI"
     gene            3736..4542
                     /locus_tag="VFD79_04260"
     CDS             3736..4542
                     /locus_tag="VFD79_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009221328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HZH93293.1"
                     /translation="MSDERIINIISNRNYESRKTKKNLMYKLRKNGFEPSETYNDKAE
                     LNICIGGDGAFLRAIHRNAFPETPFIGINTGHLGFFQELVPGEIDSFIRRYKAKDYQV
                     EKIYMVDATVCTKNKCFYLTGINEIVIKGVRSKVVHLEVFINDNHLQRFSGDGLIVST
                     PSGSTAYNFSAGGSIVYPSLESLQITPLAPINSSAYRSLPNSAIIPGDATIQIKPELR
                     YENSILVLNDGMEFKYDSIVSIDLTMSDKKIYKLNFSKDMYWKNLKSKFL"
     gene            complement(4570..5970)
                     /locus_tag="VFD79_04265"
     CDS             complement(4570..5970)
                     /locus_tag="VFD79_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZH93294.1"
                     /translation="MDSERKRIITILVALCMGFVLLIGYLSYFQMFKAQAVRDNAYNK
                     RLWINEDNILRGSIVDRGGEVLVYSESRDGETQRIYRYDNLYSHVIGYSLKDYGKSGL
                     ERRYNSYLVNANENTAINELINLINPSGVGNNLSLTLDHGLQKKTRELMNGKKGSAIA
                     INPKTGEILSMVSLPDFRIETLSENWTSVSENPDSPLLNRGTQGLYTPGSIFKILTAV
                     GVIQEGVPEAIYQCTGSTVIDGYTIRDFDESGHGPLNLEQAFAVSCNTYFADQAVALG
                     HDNLLRIAETLLFNQKIGFDTETNASVFPRESIGLTDLGASGIGQGRVLTTPLNMLMV
                     VSAVANDGYMSMPYLVETVQSPGGMTISSHRSESRKVLEIGESQALKRMMREVVESGT
                     GTNAGINGVKVAGKTGTAENASGNNHAWFVGFAPLEDPKVAVIVLLEEEGASGGRAAA
                     PIAREIINFALNNITN"
     gene            complement(5963..7198)
                     /locus_tag="VFD79_04270"
     CDS             complement(5963..7198)
                     /locus_tag="VFD79_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HZH93295.1"
                     /translation="MKERLNSKAPRKLLFLFEILSLSLIFLFSWEGIQTETYAIAVGL
                     VLLIYFSNYMLGRISKGDNYVFLIVCMLVSIGIIMIYRISPDLGLKQLMWLALGIAMF
                     FAAYFAIRYISFWDRLTPLYLVVSYGLFALTLIMGTRTHGAINWISIGGVSVQPAEIT
                     KIVLVFLLAAFYGGDEKYRRIKNAEILLMLVIYSFIGLLFLQRDLGAALIFFGIFAGL
                     QYIYTQDRRLIWANLGLAAIGGIAGYILFDHVKVRVTTWLDPWPYINDKGYQITQSLF
                     AISEGGFFGTGLGRGNPAFIPLSYNDFIFSAISEEMGIFTGIGIIMLFMLLVYRGFKI
                     ALRQDNQFFRILALGISMVFGIQSFIIIGGVLKVIPLTGLTLPFVSYGGSSVVTSFIA
                     LGILQGTSEKRGKAGIGNG"
     gene            complement(7211..7642)
                     /locus_tag="VFD79_04275"
     CDS             complement(7211..7642)
                     /locus_tag="VFD79_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZH93296.1"
                     /translation="MFDLLSIVLKYVFVFIIYLFIFTIIRMIYLDIRSVDYGNLESGA
                     YLKLLNRIDSLPYNLSDSYPVEGELTLGRQKENIIQIKDPFISKKHFRIIKDEEEFFL
                     EDLGSSNGTYLNGERVLDVVRIGKGDIIKVGELEFIFIDGA"
     gene            7787..8176
                     /locus_tag="VFD79_04280"
     CDS             7787..8176
                     /locus_tag="VFD79_04280"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HZH93297.1"
                     /translation="MKKLRKSRSDKYIFGVCGGLGEYLSIDSTIIRLIWFILALTSFG
                     TFALAYLICGLVIPEDDGYIHEDEVNGVERKDNSRTVIGIGLILVGAVLLGNMVFPWF
                     SYTMRQVMRLWPALLIVLGLYVLLKRD"
     gene            8197..8820
                     /locus_tag="VFD79_04285"
     CDS             8197..8820
                     /locus_tag="VFD79_04285"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HZH93298.1"
                     /translation="MIITNITSQRDPERVNIFIDNEFAFGLFYSTKEELGLKKGMELD
                     DALLERIKSKDELQSAKSKAYQYLSFRQRSQKELEDHLLKKEFSQTTVDCVINMLRDA
                     GYINDLDFAKAYVRDKTTFKNFGPYRIKNELSQKGISKDLIEQALQQEYQEELQELVD
                     LVKSKYSSILHDRSEKRFRRIGGFLQRKGHSYDTIKKVLDIIDGKDD"
     gene            8813..9082
                     /locus_tag="VFD79_04290"
     CDS             8813..9082
                     /locus_tag="VFD79_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HZH93299.1"
                     /translation="MTDLFYVYILKCSDNSLYTGYTDCLEKRLEKHNEGKASKYTRSR
                     LPVSMVYSEVHPDKSSAMKREIWIKSIGREGKIKLIQEASDEPLV"
     gene            9066..9632
                     /locus_tag="VFD79_04295"
     CDS             9066..9632
                     /locus_tag="VFD79_04295"
                     /inference="COORDINATES: protein motif:HMM:NF016216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HZH93300.1"
                     /translation="MNHLFNSLMEYPVIAEVNTLEEFNHALESTCQNIFMMTGNIFNL
                     RQISKKAHNKGKNIYIYIDAIQGFSKDSWGLEFISKNIEIDGIITSKPELMEQSKEMG
                     IFTLGRFSVYNEYKLGYTIEKIKETRPHCAIVLPGVLPKLIVKIIAETRIPLISSGFL
                     DNREAVIECLESGAAGISTVHFTDQNIY"
     gene            complement(9661..10071)
                     /locus_tag="VFD79_04300"
     CDS             complement(9661..10071)
                     /locus_tag="VFD79_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C biosynthesis protein"
                     /protein_id="HZH93301.1"
                     /translation="METLIGLFSKVKEILAEMGGYYDDSRMIIYVTLAVMGITLLTHL
                     IFKKIRIMKYLPGLIVLGIGLFNLYSVKDTITADASLVNLLLFIIGTVAGLVGILFAL
                     IIGIYVKPVKRKRRKAKSKEAKKPEEKTQEKPQS"
     gene            complement(10107..>11492)
                     /locus_tag="VFD79_04305"
     CDS             complement(10107..>11492)
                     /locus_tag="VFD79_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93302.1"
                     /translation="ITGKWEVVELIYSLGSGNHQEADYRVGETGLFHNDGVVLGQDYT
                     STPSFKIKKVRTRDYLISKFKLNPASLGIEEDYIKVITIINQDRYFTEFIEVDEDTLI
                     AYLDESFYRLERQLDQVSLEEVERYINVEKNVQRNLGDTIDESLSTGLLLGVRTQGFD
                     DTYQIPTWEYRTYWINMQNRNLEGLYELDRILLPRKNGFWFVDQERTVEDGIIADEIK
                     TTPLFTPDNNSEIMEAMVFDSRERSLLQERPVPSILRNILFVGNDYVSIENIELDRGD
                     RRSLHVYAMDNLADKKPIKLSDLVGETGRDLFLEGVRSVTAVNNSTLINEENVGLVRN
                     NGYWTLKGRINYKENEKELYRDFNIKAIPPKEMVSYDELAMPWDAVKLTVPDVQDVFS
                     SPNNEFMVVITTSHIVIYSLEEYDIDITPVARVRLPNDSSVIMSEWAVGRYPGIWQNE
                     MIKHGAVEVER"
BASE COUNT         3358 a         2554 c         2285 g         3295 t
ORIGIN      
        1 aataagggaa gacctgttca accatctgca gagccttgca gtcagatact ttgacaacaa
       61 tcccatcgga aggctggtca caagggtgac aaatgacaca gagacactaa atgagatgta
      121 tacaaacgta ttggtcaacc ttttcaagga ctttttcatg ctggctggaa taatgatagt
      181 aatgattagg ttgaacctcc agctggcact ggtgtccttt gccttgatac cggtgatact
      241 tctcatatca attgtattta ggaagtatat cagggaggtc tacaggatca gcagggtgca
      301 gctggccaag attaatttaa gtctgaatga gaacatatct ggaatgagaa ccatacagat
      361 attcaagaag gaaaggaaat tctttgaaaa ctttgacagt ataaacaggg actatctaaa
      421 tatccagaaa aaggaagtca catattttgc tatattcaga ccttccattg aagtgataag
      481 atcacttgga atcgcgcttt tggtctacta tggcggtggt aatgtagtct cgggaagtat
      541 ccagtttggt gtcctgttcg tatttataaa ctacctccaa aaattttatg aacccattat
      601 ggacctgact gaaaaatata acatactcca atctgccatg gcttccagtg agaggatatt
      661 caaaattcta gacaccgata tgagaatcga ggacccccat actccagcaa gaattgaaca
      721 tttcaaagga gaaatcgaat ttaggaatgt atggtttgca tataatgatg aggactgggt
      781 attgaaaaac ataagcttca aaatcaaaag gggtgaatct gttgctatcg taggagccac
      841 aggtgccggt aaaacatcaa taattaacct attgacaagg ttctatgata tccagaaggg
      901 tgaaatactc ctggatggtg ttaacataag aagcctggac aaaaacaccc tcagacagaa
      961 aattggagtt gtcctccagg atgtatttat atttacggga aatatcaggg acaatatcag
     1021 acttaacaag gacatcagtg atgacaaggt gattgaaatt gcaaagcatg tcaatgccca
     1081 cagattcata tccagattgc cacaaggtta cgatgagccg gttatggaaa gagggtctac
     1141 cctctcatca ggagagagac agctaatggc atttgcaaga accctcgttt atgatcccca
     1201 gattctggtt ttggatgagg ctacctcaag tatagacact gaaaccgaac tgttgataca
     1261 ggatgcactt cacaagcttg tggagggcag gacctccata gcaattgcac acagactatc
     1321 aaccattcaa cattcagata agattatcgt tcttaagcag ggggaaatca aggaaatggg
     1381 ttcccatcaa gacctgctta aaaataaagg tctctactat aatctgtatc agcttcaata
     1441 caaggagaac ttccagtaaa gctgaaaatc ccttgatact ttcactcttg cagagggatg
     1501 gcattaaggg tatcattgaa ttaataagaa ttaaaagaca ggagttgtaa ttaatgtacg
     1561 attttcatat tcacagtaat ttttccctgg actccaaagc caccatggag tccgtagtca
     1621 aaactgccct tgaaaagaac cttaagacca tttgctttac agaccatgtg gattttgaag
     1681 caactgaaga taaaatcgat cttcttttca gacctcttga ctattttagg gatattaata
     1741 gggtcaaata caagtatagg gacaggattg agattcttgc aggggttgaa attggaatgc
     1801 agcctggact tgaagcccgt tatgactcct ttattgagga gaatgggttt gattttgtca
     1861 taatgtccct gcataccata aatcgcaagg atatttttca tgactacagc agcaccgaat
     1921 tgtcctcctc tgaaatcata tcaaggtatt acgatgaatt gctctactgt gtgagatcct
     1981 atgacaacta cgatgttttg ggccacatag actttattga caggtacata gatgaccagg
     2041 acgtcattaa gaagtatgac ctttatatgg acaagatcga aagcatactc aggacaatca
     2101 tagacaaggg aaaaggtatt gagattaaca ctggtggtat gaggtacaga ctgaatgtac
     2161 cccaccccaa gccatctatc ctaaagcttt ataaggacct gggtggagac atcattacct
     2221 ttggatcgga ttcccatgtg gctgaccatg ttggccatga ataccgttcc agcgaaaaga
     2281 tgctcaagga aatgggattt aagtacatct atttatatag gaacaggaaa aagttcccga
     2341 tacagattca ataaaaatag ttaaattcaa ggtgaaaatg gtaaaaaaaa acacctgaag
     2401 gtgcattgta aaaaaaaagt gaaaaaattc tatactcaat aaataaaaac ccttgtaaaa
     2461 gggtttttat tttcagatta attcccgcaa tgccgtcctc tcgtctaatt cagacccgcg
     2521 taagtgcagg tgggtgtcta gccaaaaatt tctaacttcc cctcatagag ggcttgttat
     2581 ccactttcga acatctgact cccgtattta atatgtcggt tgaattatat gagaataacg
     2641 cacaccgcag aaacattcct tgagtacatt atactactta ttccactgta cttcaatgca
     2701 ttcaactaaa tttttgaaga ttttaagaaa tttaagagat ttttcatatc ttccggcatt
     2761 tctgcatgca aagatatcat ttcatctgtt cgcggagaaa caaatgacat tccataggca
     2821 tgaagtgcct gcctgtctat gtgactctcc ccatgtgagt atagatggtc tcccataatt
     2881 ggatagccca tgtgagatag atgtactcgt atctggtgtg tccttccagt gaggagttcc
     2941 agctccacca aggtcccttc ccccactctc tccagcacct tgaatctcgt cactgcctcc
     3001 gctccatcat ccctcacaac ttgtctgatg ccatcctcac ccttgcccag agctaggttt
     3061 atttcccccc agtccttagt gatatttccg tggacaatgg ccagatactt cttcactacc
     3121 ttatcatgtt ccatttgggc tgcaagctgt tggtggccaa aggagttttt gggaaatatt
     3181 attatacctg aggtgtccct gtccaatcta ttcaggattc tgacctttct ttttagtccg
     3241 cactccagaa aatagctggc caccccattt gatagggtac catcaggatg gttgggagtc
     3301 gggtgtacca gggtgaaggg ttccttgttt aaaaccagaa gatccatgtc ctcatatatt
     3361 atctccaact gcatcttctg gggcttcatg tcactgtcct catcagctag cctaatgcat
     3421 acttgcctat ccatgggtac aatttctcca cccttacatg tggaatcccc catggagatg
     3481 tccccgttaa tcagggctct ccttatcatc ctacccgata gccccttttc ccttaggaag
     3541 gaagtcaggt ctcttggggc ttttttattt gtaaatacca gtgtattagg atcttcttta
     3601 tgcattaatc ttgtcctcca tatcttttat tacttcttgt gatgtgttaa aatcattata
     3661 tcaggattaa gggtgaagtt aagttttgaa tcaaaaattg aactcaaata gtctataata
     3721 caaagggtgt gtaatatgag tgatgaaaga ataatcaaca ttatttcaaa tagaaactat
     3781 gaatccagaa aaaccaagaa gaatttaatg tataagctta gaaagaacgg ttttgaacct
     3841 tctgaaacct acaatgacaa ggctgaattg aacatctgca ttggaggtga cggagccttc
     3901 ctaagggcta tccataggaa tgccttccct gaaactccct ttataggtat taatacaggt
     3961 cacctgggat tcttccagga gctggttccg ggtgagatag attcctttat ccgcaggtac
     4021 aaggccaagg attatcaggt tgaaaaaatc tatatggtgg acgcaactgt atgtacaaaa
     4081 aacaagtgtt tctatctaac aggcataaat gaaattgtaa taaagggtgt ccgttccaag
     4141 gttgttcacc ttgaggtttt cattaatgac aaccatttac agaggttcag tggagatggc
     4201 ctgatagtat caaccccatc cggtagcact gcatacaatt tctcagctgg tggaagcata
     4261 gtatatccca gtctggaatc actccagata acacccctgg caccaatcaa ctcaagtgcc
     4321 tacagaagtc ttccaaacag tgccattatt ccaggagatg ccacaataca gattaagccg
     4381 gaattaaggt acgagaattc catcctggta ttaaatgatg gtatggaatt caagtatgac
     4441 agcattgtct caatagacct tacaatgtcg gacaaaaaaa tctataagct taacttcagt
     4501 aaggacatgt actggaagaa ccttaaaagc aagttccttt aggaagaatg ctcccaaacg
     4561 ggagcatttc taatttgtaa tattattgag ggcaaagtta attatttctc tggctattgg
     4621 tgcggctgcc ctacctcctg aagccccctc ctcctcaagt agtacaatta ccgcaacctt
     4681 gggatcctca agtggagcaa agcccacaaa ccaggcatgg ttgttcccgg atgcattttc
     4741 agccgtcccc gtcttccctg caaccttgac accattgatg cctgcattgg taccggttcc
     4801 tgattctaca acctctctca tcattctctt aagggcctgt gattctccaa tctccagcac
     4861 cttccttgat tcactcctat gggagcttat tgtcatcccc ccaggagatt gtactgtctc
     4921 taccaaatat ggcattgaca tatatccgtc attggccact gctgaaacaa ccataagcat
     4981 gttcaaggga gtcgttagaa ccctaccctg acctattcca gaagctccaa gatccgttaa
     5041 tccgatggat tcccttggaa aaaccgaggc atttgtttca gtgtcaaagc cgatcttctg
     5101 gttgaagagc aacgtctcag ctattctcag tagattgtca tggccaagtg caacagcctg
     5161 gtctgcgaag taggtgttgc atgaaaccgc aaatgcctgc tcaaggttca agggtccgtg
     5221 cccgctttca tcaaaatccc ttattgtata gccatcaatt acggtgcttc cagtgcattg
     5281 gtatattgct tccggtacac cttcctggat aactcccact gcagttagaa tcttgaagat
     5341 tgaaccgggt gtatagaggc cttgagtccc tctgttcaac aatgggctgt caggattctc
     5401 cgataccgat gtccagttct cacttaaggt ttctattctg aagtcaggta ggcttaccat
     5461 ggaaagtatt tctcccgttt ttggatttat ggcaattgcc gagcccttct tcccattcat
     5521 cagttccctg gtctttttct gaaggccatg atccagggtt agtgacagat tgttccccac
     5581 acctgaagga ttgatcagat ttattaattc atttattgcg gtattctcat ttgcattaac
     5641 caggtatgaa ttgtatcttc tttcaagacc tgattttcca taatccttta ggctgtaccc
     5701 tatgacatga ctgtatagat tgtcgtacct gtagattctt tgggtttccc catccctgct
     5761 ttcagagtag acaagaacct ccccacccct atcgacaatg cttcccctga gaatattatc
     5821 ctcattgatc cacagcctct tgttataggc attgtccctt acagcctggg ccttaaacat
     5881 ctgaaaatag cttaggtagc ctatgagaag cacaaatccc atgcagagtg ccactagaat
     5941 tgtaattatc ctttttcttt cactatccat tgccaatacc tgcctttccc cttttttcag
     6001 aggttccctg aagtataccc aaggcaataa agctggtcac aaccgagctt ccaccgtatg
     6061 acacaaatgg cagcgtcaaa ccagtaaggg gtataacctt caatactcca cctattatga
     6121 tgaaggactg gattccaaac accatactga ttcccagggc aagaatccta aagaattgat
     6181 tgtcctgcct caatgctatc ttaaatcccc tgtagaccag aagcataaac agcatgatta
     6241 tacctattcc cgtgaagatc cccatctcct cactgattgc ggaaaaaatg aaatcattgt
     6301 atgacaaggg tatgaatgca ggattacccc ttcccaagcc tgttccaaag aatcctcctt
     6361 cagaaattgc aaagagggac tgggttattt ggtacccctt gtcatttata tagggccagg
     6421 ggtcaagcca tgtggtcact ctgaccttta catggtcaaa gagaatgtat cctgcaattc
     6481 ctcctattgc ggccaaacca agatttgccc atatgaggcg tctatcctgg gtatagatgt
     6541 attgaagtcc tgcaaatatg ccgaaaaata ttaaggcagc acccagatcc ctctgcagga
     6601 acaagagacc gataaaggag tatatcacca gcataagtag aatttctgca ttcttgatcc
     6661 tacggtactt ttcatcgccg ccataaaatg cagctagaag aaagaccagg actatctttg
     6721 ttatttcagc tggttgtaca cttactccac cgattgatat ccagtttatt gccccatggg
     6781 ttctcgtacc cattatcagg gtcaaagcga acaatccata gctgactacc aggtataatg
     6841 gcgtaagtct gtcccagaag ctgatgtatc gaattgcaaa atatgcagca aagaacatgg
     6901 caatgccaag ggcaagccac attagctgct ttaatcccaa gtcgggacta attctgtata
     6961 tcatgattat tccaatggat accagcatgc acactatgag gaacacgtaa ttgtctccct
     7021 ttgatatcct accaagcata tagtttgaaa agtatataag cagaacaagt cccactgcga
     7081 ttgcatatgt ttcagtttga ataccctccc agctgaacag aaatatcaac gacagtgaga
     7141 gaatttcaaa taaaaacaag agctttctgg gggccttgct gtttagcctt tccttcatat
     7201 gatcaccttc ctaagctccg tcaataaata taaactcaag ctctccaacc tttattatgt
     7261 ctcccttccc aattctcaca acatcaagta ccctttctcc gttaaggtag gttccgttgg
     7321 agcttcccag gtcctcaagg aagaactcct cctcatcttt gattatcctg aaatgttttt
     7381 ttgagataaa gggatctttt atctggatta tattttcctt ttgccttccc aatgtcagtt
     7441 caccttcaac aggataactg tcactcaggt tgtagggcag gctatcaatc ctatttaaga
     7501 gcttcaggta tgctcccgac tccaggttgc cataatccac actcctgatg tccaaatata
     7561 tcattctaat gatggtgaaa ataaatagat aaattataaa tacaaacaca tacttcagaa
     7621 ctattgaaag caaatcaaac attatatcac cccctattta tgttaccaca aaccactatc
     7681 caagggaatt aaaacccacg gtaatttgat aattttcagc ttcctatttc aataaccccg
     7741 aaaatctgat attatcaaaa tgagaattaa tatgggaggt aggataatga aaaagttgcg
     7801 aaaatcaaga tcagacaaat acattttcgg agtatgcggt ggtctgggag aataccttag
     7861 catcgattcg acaataatca ggctaatctg gtttattttg gccctcactt catttggaac
     7921 atttgccctg gcatatctta tatgcggatt ggtgattcca gaggacgatg gctacatcca
     7981 tgaggatgag gttaatgggg ttgagcgaaa ggacaatagc aggaccgtaa tcggaatcgg
     8041 cttaatcctg gttggggctg tactcctggg caatatggtc ttcccctggt tttcctacac
     8101 catgcgtcag gttatgaggc tatggccagc actgctgatt gtcttgggat tgtatgttct
     8161 acttaagcga gactagattg tttgaaggga agcattatga taattacaaa tataacctca
     8221 caaagggatc ctgaaagggt caacatcttt atcgacaatg aatttgcctt tggtttgttc
     8281 tattcaacca aggaagaact tggcttgaaa aagggtatgg agctggatga tgcacttctt
     8341 gaaaggataa agtccaagga tgagcttcaa agcgccaagt ccaaggcata ccaatacctt
     8401 agctttcgac agcgttccca gaaggaattg gaggatcatc tccttaagaa agagttcagc
     8461 caaaccactg ttgattgtgt aattaatatg ctaagggatg ctggctacat caatgatctg
     8521 gactttgcaa aggcctacgt cagggacaag accaccttca agaattttgg cccctacagg
     8581 atcaagaacg aattgtccca aaagggcata tccaaggacc tgatagagca ggcgctccag
     8641 caggagtacc aggaggagct tcaagaactt gtggatcttg tgaaatccaa atattcatca
     8701 atactccatg acaggtcgga gaaaaggttc agaagaatcg gtgggttttt acagagaaaa
     8761 ggccattcct atgatacaat caaaaaggta ctggacataa tagatggaaa ggatgactga
     8821 tttgttttat gtatatatcc taaaatgcag tgacaacagc ctttacacag gctacacgga
     8881 ctgccttgaa aagcgtttgg aaaaacacaa tgaaggaaag gcatcaaaat atacaaggtc
     8941 aaggctccca gtcagtatgg tttactccga agtccatcct gacaagagtt cagcaatgaa
     9001 gcgggaaatt tggataaaat caataggccg tgagggtaag attaaattga ttcaggaggc
     9061 atcagatgaa ccacttgttt aacagcttaa tggaatatcc agtgattgct gaggtcaata
     9121 cacttgaaga gttcaaccat gctctggaat ccacttgcca aaatattttc atgatgactg
     9181 gaaacatatt caacctgaga cagatatcaa aaaaggccca caacaaaggg aagaacatat
     9241 acatatacat tgacgcaatc cagggctttt ccaaggattc ctggggcctt gaattcatat
     9301 ccaaaaacat cgaaattgac ggcataataa catccaaacc cgagcttatg gaacagagca
     9361 aggagatggg aatcttcacc ctggggagat tttcagtata caacgaatac aaattaggct
     9421 acaccataga aaaaatcaag gaaaccagac cccactgcgc aatagtcctt cctggagttt
     9481 tacccaagct tattgtgaaa atcattgctg aaaccaggat cccactgatt tcaagcggtt
     9541 ttttggataa tcgcgaagcg gtaatagaat gcctggagtc tggtgcagct ggaatatcga
     9601 cagtacattt tactgaccag aacatatatt aaccctgaat cttatgattc agggtttctt
     9661 ttagctttga ggcttttctt gggtcttttc ttcaggtttc ttcgcttcct tgctcttggc
     9721 ttttcttctc tttctcttaa ccggctttac atatatgcct atgataaggg caaagaggat
     9781 tcccacaagc ccagccaccg ttcctattat gaacagcaaa aggttcacaa ggcttgcatc
     9841 ggcagttatg gtatccttta cactgtagag gttgaacagc cctatcccga gaactatgag
     9901 ccctgggaga tatttcataa tcctgatctt cttgaaaatc agatgtgtaa gcagggttat
     9961 tcccatgact gccaatgtaa cgtaaattat catccttgag tcatcgtagt agccacccat
    10021 ttcagccaat atttccttaa cctttgaaaa caggccaatc aacgtctcca taaaatcatt
    10081 cccttcagtt attatgtctc acaaggctac cgttcaactt caacagctcc atgttttatc
    10141 atttcgtttt gccatattcc agggtatctt cccactgccc attctgacat tatgacactt
    10201 gaatcattgg gcagccttac ccttgcaaca ggtgtaatat ctatatcata ctcctccaag
    10261 gaatatatta caatatggga agtggttatt acaaccataa attcgttgtt gggggatgaa
    10321 aatacatcct gtacatccgg gactgtaagc ttgactgcat cccatggcat agccagctca
    10381 tcatagctta ccatttcctt tggtggtatg gccttgatat tgaagtccct gtaaagctcc
    10441 ttttcgtttt ccttgtagtt tatcctgccc ttcagagtcc aatacccgtt gtttctcacc
    10501 aggccaacat tctcctcgtt tatcaatgta ctgttattga ctgctgtgac acttctgacc
    10561 ccttcaagaa acaggtccct acccgtttca cctaccaggt ctgacagctt gattggcttc
    10621 ttgtcagcaa gattgtccat tgcatacacg tggagggacc ttcggtctcc cctgtcaagc
    10681 tcgatgttct ctatggaaac ataatcattg cccacaaaaa ggatgtttct taggattgat
    10741 ggaaccggcc tctcttggag caagctcctc tccctggagt caaataccat ggcctccatg
    10801 atttccgaat tgttatccgg tgtgaatagt ggcgtcgtct tgatctcatc tgctatgatt
    10861 ccatcctcaa cggtccgttc ctggtcaaca aaccagaaac catttttcct tggcaggagg
    10921 atcctatcca gttcatacag gccctcaaga ttcctgtttt gcatatttat ccaataggtt
    10981 ctgtattccc aggttggtat ctggtaggta tcgtcaaagc cctgggtcct gacaccaagc
    11041 agcaggcccg tactgagact ctcatcaatt gtatcaccaa ggttcctctg gacattcttc
    11101 tccacgttga tgtatctttc aacctcttca aggcttacct ggtccagctg cctttccagt
    11161 ctgtagaagc tttcatccaa ataggcaatc agggtatcct catcaacctc tatgaattca
    11221 gtaaaatacc ggtcttggtt tattatggtg attaccttga tgtaatcctc ttctattccc
    11281 agcgaagctg ggttaagctt gaatttagat atgagatagt ctctggtcct gaccttcttg
    11341 atcttaaatg aaggggttga tgtgtagtcc tgaccaagaa ccaccccatc attatggaat
    11401 agaccagtct cacctacacg gtagtcagct tcctggtggt ttcctgaacc taggctgtat
    11461 attaactcaa ctacttccca tttcccggtt at
//
LOCUS       DASRQF010000156         4801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_2578, whole genome shotgun sequence.
ACCESSION   DASRQF010000156 DASRQF010000000
VERSION     DASRQF010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2578"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..827)
                     /locus_tag="VFD79_04310"
     CDS             complement(36..827)
                     /locus_tag="VFD79_04310"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HZH93303.1"
                     /translation="MLEKCIARARNAEIYSWEDNLVLKLFYSFIPRESVESEYLKTRL
                     IRDKTPICPDVYDRISLEEGEGIVFENIEGKSITYNLLNNPLRLREYATIMAQTHKSI
                     HKITVDGLPELNIEIGSIIDKSSRLDDYQKIKLKKLLSNLPNDNKLCHMNFHPDSIFI
                     GKKGPKVIDWMNASLGNPLLDVARTVVLLRFSSLPFQNILFRGVLKLSREILIKEYLD
                     SYFQEKKYEENDFNSCVLLAAAARTCEKLPQSEIEKLQTFLQEEL"
     gene            complement(918..2882)
                     /locus_tag="VFD79_04315"
     CDS             complement(918..2882)
                     /locus_tag="VFD79_04315"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZH93304.1"
                     /translation="MKRRGLFFLFTIVILIISTPFAQASEAPDFYDLVDSHGSMMMFI
                     DPSNGRIVYVNQAAVDFYGYDKDVLESMDVYDLRTGTSYDKVPSWKELNSRDKNTYTV
                     QHRIADGSTRSLEVKSYPYEYAGRTVLFSILEDITQRTILEESNRQQMNIIISGALIS
                     IAVLLYIAEARRKINEDIEEINSQLNSSKDKLQLILDSTVEGIYGMDREGVCTFINQS
                     GLKILGYTSQEDLLGKNMHTQIHHSHRNGEVMPVDQCKILKSINEGKPAYVNDEVFWR
                     KDGSFFDVEYDSHPQQENGEIVGAVVTFNDITEKRKIEREIQYLSFHDPVTDLYNKTF
                     FQEELKRLDVERNLPISVIVGDINGLKLTNDILGHEAGDELIRAAAQTMKEVCRADDI
                     IARTGGDEFTVLLPSTSLQEAKKIGERIKENIEAKKINMFKGSISIGCAMKTEAGESI
                     DEVLNRADKRMYLDKTLNNNTVSKDQLENLMTSLHERSKREKTHAEETARISGLIGKS
                     LNLRPEILKRLKDAAYYHDIGKIVLSDDILTKKGDLTEDEQIEMEQHPIAGYRILNLF
                     NETVNIAEIVFAHHERWDGKGYPKGLNGIEIPVLSRIISVSEVYDFLTRDKDGSKLTR
                     EEALRRFESEAGSRFDPMVTGALARIMDEI"
     gene            complement(2903..>4801)
                     /locus_tag="VFD79_04320"
     CDS             complement(2903..>4801)
                     /locus_tag="VFD79_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZH93305.1"
                     /translation="NDGEYIGWKAPEGHPVYEFMRNPLEFYSEEIRQDSESSQYYKYG
                     YFQRDDGSFVQVGIYAEEINDFIGSFEMQQLLDEIKDIQLVHSLYFIDKNLEIISATI
                     DEYIGTTIMNPDIVDAIRRNKEYNSTVEIYGEELYYGMVPIHIENDKIGTLAVAQSTG
                     DMDLVVSDAVRNGIYNLASVFLVIGFFASYVIRKNKQLVEMAYYDKLTGLPNQAYLKE
                     YIQETIKEKAVEDKAVLLVNTQNFRHINMTYGYDFGDDILREIARRLKSVIGDSHMVS
                     RFTSDRFVVFVEGYHGKEQLVKLTESITASLDKPIENSRSRQYINSQIAIIDSIKGYF
                     DVDNLLREASIALDHLPRESSLNYIFFSEEMGDRVNRWDAIENEIRDFVLTPDSESIY
                     LEYQPKVDLRTGKISGFEALARMNSAELGRISPIEFIDVAEKRLMIAPLGYRLLEMAA
                     KFAKEMDCEICRNTTIAVNISGIQLLRDDFVDRVKGIIDRYDLEPGRLELEITESILL
                     DNFNVINDKLKILKDSGIEISLDDFGTGYSSLSRLGELNVGVIKIDRYFIKKIDDLTE
                     DSHLAHDIISMAHKSGLKVVAEGVETMDQLEYLRRNHCDYMQGYLFSRPVSPEEALKL
                     LQSTNKDK"
BASE COUNT         1237 a         1152 c          851 g         1561 t
ORIGIN      
        1 cttaaccata accccatcct acacttacca gtcctctaaa gttcctcctg aagaaatgtc
       61 tgtaacttct caatttcaga ttgtgggagc ttttcacagg ttctcgccgc agcagccagc
      121 agcacacagg aattgaaatc attctcttca tattttttct cctgaaaata tgaatccaga
      181 tattccttga ttagaatttc acggctcagc ttaaggaccc ccctaaataa aatgttctga
      241 aatggcaggg atgagaacct aagcaaaact acagttctgg caacatccaa taaagggtta
      301 cccaaactgg cattcatcca gtcaatcacc tttggtccct tcttgccgat gaaaatacta
      361 tcgggatgaa aattcatatg gcatagcttg ttatcattcg gtagattact taagagtttt
      421 ttaagcttaa tcttctgata atcatcaagc ctggatgact tatctattat agagccaatt
      481 tcaatattaa gctcgggtag tccatcaacg gtaattttat gtatgctttt atgggtttga
      541 gccattatgg ttgcatactc ccttagccgt aaagggttgt tcaataggtt gtatgtaatg
      601 gatttacctt caatgttttc aaaaacaatt ccttctccct cctccagtga gatcctgtca
      661 tagacatcgg gacatatggg agttttatct ctaataagac gggtctttaa atattcactt
      721 tccacggatt ccctaggaat aaaggaataa aaaagcttca ggaccaggtt gtcctcccag
      781 gaatatattt cggcatttct ggctcttgct atacactttt ccagcatctc acacctcctt
      841 taatagagat accctgtttt tcttcatata tacagttttg gacaaagaaa aagagagagc
      901 tgttaaccct ctcagtccta tatctcatcc attatcctag ccaatgcccc tgttaccatt
      961 gggtcaaacc tacttccagc ttcagactca aaccgtctta gggcctcttc ccttgtaagc
     1021 ttgcttccat ccttgtccct tgtgaggaaa tcatagacct ccgagacaga aattatcctt
     1081 gaaagcactg gaatctctat accatttaga ccctttgggt atcccttccc atcccacctt
     1141 tcatgatgag caaaaactat ttcagctatg tttacagttt cattgaatag gtttaggatt
     1201 ctgtagccag caattggatg ctgctccatc tcaatctgct cgtcttctgt caagtcaccc
     1261 tttttggtca ggatatcatc tgaaaggact attttgccaa tatcgtgata ataggctgca
     1321 tcctttagcc tttttaatat ttccggccta agattaaggg atttaccaat caaaccgctg
     1381 atcctcgcag tctcctcagc atgggtcttc tccctcttgc tcctttcatg aagggatgtc
     1441 atcaggtttt caagctggtc ttttgaaact gtattgttgt ttaatgtctt gtcaagatac
     1501 atccttttgt cagccctgtt caatacctca tctatgcttt cacctgcctc ggtcttcata
     1561 gcacagccaa tggagatgct tcccttgaac atattgattt ttttcgcctc aatgttctcc
     1621 tttatccgtt caccaatctt tttagcctcc tggaggcttg ttgatggcag taatacggta
     1681 aattcatctc cacctgtcct ggcgatgatg tcatcagccc tgcagacctc cttcattgtc
     1741 tgggcagcgg cccttatcag ttcatcccct gcttcgtggc caagaatatc attggtaagc
     1801 ttaagaccat tgatgtcccc tacgattaca gaaatgggaa gattcctttc cacatcaagc
     1861 ctcttcagct cctcctggaa aaatgtcttg ttgtaaaggt cagttacagg gtcatggaaa
     1921 ctgagatact gaatctctct ttcaattttc cttttttcag taatgtcatt gaaggtaaca
     1981 accgcaccta caatttctcc attctcttgc tggggatggg aatcgtactc aacatcaaaa
     2041 aaagacccat ccttgcgcca gaaaacttca tcattgacat atgctggttt cccctcatta
     2101 atggacttga gaatcttgca ttgatcaacc ggcatgacct caccgtttct gtgggagtgg
     2161 tggatctgag tatgcatatt tttgcctaat aggtcttcct ggctggtgta gcccaggatc
     2221 ttaagaccgc tttggtttat gaatgtacaa acaccttccc tgtccattcc gtagattccc
     2281 tctacagttg agtccagaat caactgtagc ttgtccttgc tgctgtttag ctgggagttg
     2341 atttcttcta tatcctcatt tatctttctt ctggcttccg ctatatacaa aagaacagct
     2401 atgcttatca aggctccgct gattattatg ttcatctgtt gtctgttgct ctcctcaaga
     2461 attgtcctct gtgttatatc ctcaaggatg gagaaaagca ctgttcttcc tgcatactca
     2521 taggggtagg acttaacctc cagggatctg gtactcccat ctgcaattct atgctggacg
     2581 gtatatgtgt ttttatccct tgagttgagt tccttccaac ttggaacctt gtcatagctc
     2641 gtgccagtcc tcaaatcata gacatccatg gattccaaaa catctttatc ataaccatag
     2701 aaatcaacag ctgcctggtt gacatagact atccttccat tgctggggtc aatgaacatc
     2761 atcatggatc cgtggctatc cacaagatca tagaaatcag gcgcttcact tgcctgggca
     2821 aatggggtgg atataatcaa tatcactata gtaaaaagga aaaataatcc tcttcttttc
     2881 atttcattcc caccattcaa aattatttgt ccttattggt actttgtaga agcttcaagg
     2941 cttcttctgg agaaactggc ctgctgaaaa ggtaaccctg catatagtca caatggtttc
     3001 tccttaggta ctcaagctga tccattgtct caaccccttc agccacaacc ttgagaccgc
     3061 tcttgtgggc cattgaaata atgtcgtggg ccaggtggct gtcttcagtt agatcatcta
     3121 tttttttaat gaagtaccta tcaatcttta taactccaac attcagctcc cctagtcttg
     3181 aaagagagga atatcctgtt ccaaagtcat ccagggaaat ttctatacca gagtccttta
     3241 atatcttaag cttatcattt ataacattga aattatcaag taggattgac tcagtaatct
     3301 caagctccag ccttccaggc tcaaggtcat acctgtcaat tatacccttt acacggtcta
     3361 caaagtcatc cctcaacagc tggatccccg aaatattcac tgcaattgtc gtattcctgc
     3421 agatttcaca atccatttcc ttggcgaact ttgcagccat ttccagaagc ctatatccta
     3481 gtggggctat cattagtctc ttttctgcaa catcaataaa ctctattggt gaaatccttc
     3541 caagctcagc tgagttcatt cttgcaaggg cttcgaagcc agatatttta cctgttctta
     3601 aatcaacctt cggctggtat tctagatata tcgactcaga atcaggtgtc aggacaaaat
     3661 ctctgatttc attttctatt gcatcccacc tgtttacccg atcacccatt tcttcactga
     3721 agaagatgta attcaacgag gattccctag gaaggtggtc cagagcaatt gatgcctccc
     3781 ttagaagatt atccacatca aagtagccct taatcgagtc gattatggct atctggctat
     3841 ttatgtactg tctgctccta gagttttcta taggcttgtc cagggaagct gttattgatt
     3901 cagttagctt tacaagctgt tccttaccat ggtagccttc aacaaaaaca acaaacctgt
     3961 ctgatgtgaa ccttgaaacc atgtgactgt ccccgattac agacttgagc ctccttgcaa
     4021 tctcccttaa tatatcgtct ccaaaatcgt atccgtaggt catgttgatg tgtctgaagt
     4081 tttgtgtatt cacaaggagt actgccttgt cctccaccgc tttttccttg atggtttcct
     4141 ggatgtattc ctttagatag gcctggtttg gtagtccggt aagcttgtcg taatatgcca
     4201 tttccaccag ctgcttgttt tttctgatta catatgatgc aaagaatccg attactagaa
     4261 aaacgcttgc cagattatat attccattcc taacggcatc actaacaaca aggtccatgt
     4321 ccccagtaga ttgggctaca gccagagttc caatcttgtc attttctatg tgaattggaa
     4381 ccattccata gtagagctcc tcgccatata tctccactgt ggaattgtat tccttgttcc
     4441 ttcttattgc atctacaatg tcaggattca tgatggttgt tccaatgtat tcatcaattg
     4501 ttgcactgat tatctcaaga tttttatcaa tgaaatagag gctgtggaca agctggatat
     4561 cctttatctc atcaagcagc tgctgcattt cgaagcttcc gatgaaatcg ttaatttctt
     4621 cagcatatat ccccacctgg acaaagctcc catcatctct ctgaaaatat ccgtacttgt
     4681 aatattggct actttcagaa tcctgtctaa tttcctcaga gtagaactcc aagggattcc
     4741 tcatgaactc atacacgggg tgtccttcag gagccttcca gccaatgtat tcaccatcgt
     4801 t
//
LOCUS       DASRQF010000157         5638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1377682, whole genome shotgun sequence.
ACCESSION   DASRQF010000157 DASRQF010000000
VERSION     DASRQF010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377682"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VFD79_04325"
     CDS             <1..537
                     /locus_tag="VFD79_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93306.1"
                     /translation="ASLGLKALLVGYETFDDGELVKYRKKSTTDENREAARLLQEIGI
                     DVWASFMAHPDWTREDFRTFRRYIRELAPEISTINPLTPFPGLPLFEKYKDRLLYRVE
                     DYHRWSFGQVMIMPSKMSLGSYYMELLKTYLYINLALNRNTEMIRKYGLINILRILKG
                     SVGASFKYIRLMRSQRLT"
     gene            611..1558
                     /locus_tag="VFD79_04330"
     CDS             611..1558
                     /locus_tag="VFD79_04330"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HZH93307.1"
                     /translation="MRKRLILIVGIMTLVLLLSACGPASERAIKNADDPIVLGSFIDT
                     EGGILGNMILLALEDKGYEIVDKVQFGTPDVHRNALLEGELHIGIDYTGNGQFYSEGF
                     QEDLWRDADEGYQAIKGYDEENNNLIWLSPAKANNTEALAVKREFAQENDITDMYDFA
                     EYVNNGGYVKFITSQLFAEKYTGLLGMEEAYGFTLNPDQLIMLPHGNTAETLSALANE
                     TDGVNVALAYGTDGSIADLDLVVIDDPLSIPPVYEPSAIVRGEVLEKYPEIQEIIEEV
                     FATLNLENLQEMNKMVIVDGASPRDVAEDHLLQNGMIEE"
     gene            1576..2727
                     /locus_tag="VFD79_04335"
     CDS             1576..2727
                     /locus_tag="VFD79_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZH93308.1"
                     /translation="MADKKDRVLILLSALGTAGFFLLPLVEAKPNRIVSGEKLLALEY
                     LGVLGALIIMGWAMLMALSLYGTKRKNEYISIAAIITAVGLVVLLPIGGGSIQEIGAN
                     PRISFSSGFYIQVLFLYIILSTYLPRVGWKRRLKLLLGVGALLVMASLMYMGYFERLS
                     IIKEYGVRQRQFYNNLYIHGVLAFGSVLAGALIAIPLGYLAYIKKRLEGRIMVPLSII
                     ETIPSLSLFGILLVPLSGLGQLPFFRAIGVSGIGWAPAFVALTLYTLLPIGRNTLTGF
                     HSIDKNVVQAAKGMGMSRRQILRKIEIPLAFPVIFTGIRIAFIQTIGGAVLAGLVGGG
                     GMGSFVFLGLAEASPDLILLGVIPIVFFTVVLDYSLKVFERYVRRVVYD"
     gene            2720..3649
                     /locus_tag="VFD79_04340"
     CDS             2720..3649
                     /locus_tag="VFD79_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZH93309.1"
                     /translation="MIEMQNVTKSYGDFKAVDNLSLHVDRGELLVILGPSGCGKSTTL
                     RLMNKMAERSSGTIRIMEKDIDSFVEEDLRKGIGYVIQSIGLFPHMNVWKNIATVPQL
                     LGWDEKRIDGRVQELLLLVGLDPDKYKYKKPSQLSGGEAQRVGVARALASDPDILLMD
                     EPFGAVDPINRLRLQKEFRQIQRRLQKTVVFVTHDIDEAILLGDRICIMNHGRVVSID
                     TPEKVVLHSKDDFVDSFFKGEGILTILSRKNVGDYMEEIPCKGEEMEEDSTLKEALSL
                     MLLTGRDRIPVKGGSISFNRILQVVGRDINEKA"
     gene            3636..4364
                     /locus_tag="VFD79_04345"
     CDS             3636..4364
                     /locus_tag="VFD79_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZH93310.1"
                     /translation="MKRLNVNIIVALILTLILAMIVVYSREFEGLLYRYFSTKNTTGS
                     RTPLLVYFGQHIWMVLVSSVIAVGIGLGLGIFATTKTGEDFRDPLLKVVNLGQAFPSP
                     ALLALVVPILGYGHEGALIALVVYALMPIVYNVVVGIEEVAEDVVDAAIGMGMTPFQL
                     YRKIKIPLAMNVILGGIRTALVINISAATLAAAVGAGGLGVLVVNGVRTFDMVLILQG
                     AIPVTIMAVIVELLLKEVQARLSF"
     gene            complement(4407..4856)
                     /locus_tag="VFD79_04350"
     CDS             complement(4407..4856)
                     /locus_tag="VFD79_04350"
                     /inference="COORDINATES: protein motif:HMM:NF018448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HZH93311.1"
                     /translation="MYILTQGFYSLLCVIGFSIIFNLPRRLVPLASLSGMLGWIVYIL
                     LQQTATSFIIPAFVGSIFVGLVGELAAIYKKQPATIFIIPGIIPFVPGYGIYYTMFHI
                     IDNNFSQALVTGGESLFIAISIACGVVIATSVVRYIRPYLSQGDGSF"
     gene            complement(4867..>5638)
                     /locus_tag="VFD79_04355"
     CDS             complement(4867..>5638)
                     /locus_tag="VFD79_04355"
                     /inference="COORDINATES: protein motif:HMM:NF018448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HZH93312.1"
                     /translation="EKTLKRAIATAVETGMIMLQNGAETYRVEETINRMISSKTNSPV
                     EVFVMSTGIIVGAHINGEPYTTVSRVPSIGLDLETIARANSFSRIFTQGDMTQEETDI
                     ILGELRSPPKFSKPIRYAFSGMAGGFFVLMFGGSFVEFVLAYMASSITVFSMDKLTVR
                     RLNFFVKNILGGLISALLSIFLVFVVGGLSITADFNTVIIGPLMTLVPGVSLTNGIRD
                     LISGELIAGTTKMMEALFIAIALAFGVGIVLQGIIAFI"
BASE COUNT         1564 a         1218 c         1439 g         1417 t
ORIGIN      
        1 gcatcccttg gtctcaaggc tcttttggtg gggtatgaaa ccttcgatga tggggagctg
       61 gtcaagtaca ggaagaagtc cacaacagat gagaacaggg aggctgcaag gcttcttcag
      121 gaaataggca tagatgtttg ggcatccttt atggcccatc ccgactggac cagggaagac
      181 ttcaggacct tcagaaggta tatcagggaa ctggctccgg aaatatcaac aatcaacccc
      241 ctcaccccct tcccaggtct gcccctgttc gaaaagtaca aagacagact gctgtacagg
      301 gtggaggatt atcataggtg gagctttggc caggttatga taatgccctc caaaatgtcc
      361 ctcgggtcct attatatgga gctcctgaaa acatacctct acataaacct tgccctcaac
      421 agaaacacgg agatgataag aaagtacggc ctcattaata tcctaaggat cctcaagggt
      481 tccgttggag cctcattcaa atacatccgc ctgatgagat cccagcgact cacttgatta
      541 attacctagc aatcgggtat gtattgacca tggattagct tgttaaagca tgaaatggag
      601 gaggagaaaa atgagaaaga gactcatttt aatagttgga ataatgaccc tggtcctttt
      661 gctgtcagcc tgtggaccag ctagtgaaag agcaataaag aatgcggatg acccaatagt
      721 cctgggatcc tttattgata ccgaaggagg gattcttggg aacatgatac tccttgcact
      781 tgaggataag ggctatgaaa ttgtggataa ggttcagttt ggaacgccag atgtccacag
      841 gaatgcactt cttgaaggag agctccacat aggaatcgac tacaccggca acggccagtt
      901 ctactcggag ggcttccagg aagacctgtg gcgggatgca gatgagggat accaggcaat
      961 aaagggctac gatgaggaga acaacaatct catatggcta agccctgcca aggcaaacaa
     1021 cacggaagcc cttgctgtga agagggaatt tgcccaagag aatgacatca cagatatgta
     1081 cgattttgca gagtatgtga acaatggcgg ctacgtgaag tttatcacca gccagctttt
     1141 cgcggagaaa tacacaggcc ttctgggaat ggaggaagcc tatgggttta cactcaatcc
     1201 agaccagctg attatgctgc cacatggcaa tacagctgag accttgagcg cattggccaa
     1261 cgagaccgat ggagtcaatg ttgccttggc ctatggtacg gatggttcta tagcagacct
     1321 tgaccttgtg gtaatagacg atcccctgtc aatacctcct gtatatgagc cctcggctat
     1381 tgtaagaggc gaggtgcttg aaaagtatcc tgaaatccag gagatcattg aggaggtttt
     1441 cgccaccttg aacctggaga accttcagga aatgaacaag atggtgatag ttgacggcgc
     1501 atcacctagg gatgttgcag aggaccacct tcttcagaac ggaatgattg aagaatagct
     1561 agagtgggtg atactatggc agataaaaaa gaccgggtat tgattctact atcagccctg
     1621 ggaaccgcag gcttcttcct gcttcccctg gtggaggcaa agccaaacag gattgtatcg
     1681 ggagagaagc tgcttgcact tgagtatctt ggcgtattgg gagctttgat tattatggga
     1741 tgggccatgt taatggccct ttctttgtat ggaacaaaaa gaaagaatga atacatatcc
     1801 atagccgcaa ttattacagc tgtaggcctt gttgttcttc ttccaattgg gggaggaagc
     1861 atccaggaaa tcggtgcaaa cccaagaatc agcttttcat caggtttcta tatacaggtt
     1921 ctctttctgt acataatact atcgacctac cttcccaggg ttggttggaa aagaaggctc
     1981 aaactccttc tgggagtcgg agccttactt gtcatggcaa gtcttatgta catgggatac
     2041 tttgaaagac tttcaattat caaggagtat ggggtccggc aaaggcaatt ctacaacaat
     2101 ctctacatcc acggggtcct tgccttcgga tccgttctgg caggggccct aatagccata
     2161 cccctgggat accttgcata catcaagaag agactggagg gaaggataat ggtccccttg
     2221 agcataattg agaccatacc aagcctatcc ctctttggga ttctccttgt acctctttca
     2281 ggacttggac aacttccatt tttcagggca attggggtaa gtgggatcgg atgggctccg
     2341 gcctttgtgg ccctaaccct ctacaccctc ctgccaatag gaagaaatac actgaccggc
     2401 tttcattcaa tagataaaaa cgtcgttcag gctgccaaag gaatgggaat gagcagaaga
     2461 cagatcctga gaaagattga gatacccctg gcctttccag ttatatttac tggaatccgc
     2521 attgccttta tccagacaat aggtggagcg gtcttggcag gtcttgtggg aggtggagga
     2581 atgggaagct tcgtattttt gggattggca gaggcatccc cggacctgat ccttctggga
     2641 gtaatcccca tagtgttttt taccgttgtt ctggactatt cactaaaggt ttttgagaga
     2701 tatgtaagga gggttgttta tgattgaaat gcagaatgta acaaaatcat atggagattt
     2761 caaggctgtg gacaatctat cccttcatgt tgacaggggg gaacttctgg taatactggg
     2821 gccttcgggc tgtggaaagt cgactacatt gaggctgatg aacaaaatgg cagaaaggtc
     2881 ctcggggacc ataagaataa tggaaaagga tattgattcc tttgtggagg aggacctaag
     2941 gaagggaata ggctatgtca tccaaagcat aggtcttttt ccacatatga acgtatggaa
     3001 aaatattgct actgtacccc aactcctggg ctgggatgaa aagcggattg acggaagggt
     3061 ccaagagttg ctccttctgg ttggactgga cccggataag tacaagtaca agaaaccatc
     3121 acagctgtca ggcggcgagg ctcagagagt gggcgtggca agggcccttg catctgatcc
     3181 tgatatcctg cttatggatg agccctttgg ggcggtggac cccataaaca ggctgaggct
     3241 acaaaaggag ttcaggcaaa tccagagacg gctccagaag actgtggtct ttgttaccca
     3301 tgatatagac gaggccatac tccttgggga caggatatgc atcatgaacc atggaagggt
     3361 ggtaagcata gatacccctg aaaaggtggt cctccattca aaggatgact ttgtggatag
     3421 cttctttaag ggggagggga tccttacaat cctgtcaagg aagaacgttg gtgactacat
     3481 ggaggaaatt ccatgtaaag gtgaggagat ggaagaggat tccaccttga aggaggcact
     3541 ttccctgatg ctacttacag gtagggacag gataccggta aaaggcggaa gcatttcatt
     3601 taacagaatc ctacaggtgg ttgggaggga tataaatgaa aaggcttaat gtgaatataa
     3661 ttgttgccct tattctgacc ctgatacttg ccatgatcgt agtctattca agggaatttg
     3721 agggcctctt atacaggtat ttcagcacaa aaaacactac cggctccaga accccattgc
     3781 ttgtatactt tggacagcac atttggatgg tacttgtttc aagtgttata gcagttggga
     3841 ttggacttgg ccttggaatt tttgcaacca ccaagaccgg tgaagacttc agggaccccc
     3901 tgctcaaggt tgtaaacctg ggtcaggcat ttccatctcc ggcccttttg gcactggtgg
     3961 ttccaatact gggctatggc catgagggag ccctgattgc cctggtggtg tatgccttga
     4021 tgccaatagt gtacaatgtt gtggtaggca ttgaggaggt agctgaggat gttgtggatg
     4081 ctgccattgg aatgggcatg acccccttcc agctctacag aaaaataaaa atcccccttg
     4141 ccatgaatgt aatcctgggg ggaataagaa cagccctagt tatcaatata agtgccgcaa
     4201 cccttgcggc tgcagtaggt gcgggaggac tgggggtgct tgtggttaac ggagtgagaa
     4261 cctttgacat ggtccttatc ctccagggag caattcccgt aaccataatg gcggttatag
     4321 tcgagctcct tcttaaggag gtccaggcta gactgtcatt ctagaccggg gggacgtacc
     4381 cagtgggcac attttgggaa agtgagtcaa aaagaaccgt ccccctgact cagatagggg
     4441 cggatatacc gtacaacaga ggttgcaatt actacgccgc aggctattga gattgcaatg
     4501 aaaagggatt ccccgcctgt gacaagagcc tgggaaaagt tgttgtcgat aatgtgaaac
     4561 atggtgtaat agattccata gcctggtaca aatggtatta ttcctggaat aataaatatt
     4621 gtagcaggct gttttttgta gattgcagcc aattctccca caagacccac aaaaatggac
     4681 cccacaaatg ccgggataat aaagcttgtg gcagtctgct gaagaagtat gtagactatc
     4741 cagcccagca ttccgcttag ggatgccagg ggaacaagcc ttcttggcag gttgaatatt
     4801 attgaaaaac cgattacaca caagagggaa tagaatccct gggttagaat gtacataaaa
     4861 atcctcctat atgaacgcaa tgattccctg aagcacgata cccactccaa aggcaagggc
     4921 tattgcaatg aaaagtgcct ccatcatctt tgtggtccct gcaataagct ctccggagat
     4981 caggtctctt attccattgg taagggaaac ccctggtaca agggtcatca agggacctat
     5041 tataacagtg ttaaaatcag cggtaattga aagaccgccc accacaaaga ccaagaatat
     5101 gcttaagagg gctgaaatca atcccccaag gatattcttg acaaagaaat tcagccttct
     5161 aactgtaagc ttgtccattg agaagaccgt aatgctggat gccatatacg ccaggacaaa
     5221 ctccacaaag ctacctccaa acatcagcac aaagaagcct cctgccatac cgctgaaggc
     5281 atacctaatg ggctttgaaa acttgggtgg tgaccttagc tcacctagga ttatgtcagt
     5341 ttcctcctgg gtcatgtccc cttgggtaaa gatcctggaa aaggagtttg cccttgctat
     5401 tgtctcaagg tcaagtccaa ttgaaggcac cctggaaaca gttgtatagg gctctccatt
     5461 tatatgggct ccaactatta tccctgtgga catgacaaat acctccacag gagaatttgt
     5521 ctttgaggag atcatcctgt tgatggtctc ctcaacccgg taggtttcag ccccgttctg
     5581 gagcataatc atgccggttt ccactgctgt tgcaattgct cttttcaatg tcttttct
//
LOCUS       DASRQF010000158         2658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1080399, whole genome shotgun sequence.
ACCESSION   DASRQF010000158 DASRQF010000000
VERSION     DASRQF010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080399"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            502..1911
                     /locus_tag="VFD79_04360"
     CDS             502..1911
                     /locus_tag="VFD79_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HZH93313.1"
                     /translation="MEKKQRENWGSRIGFIMAAAGSAVGLGNIWRFPYLAGENGGSAF
                     ILIYLGFVLVIGLSIMVSELAVGRRTELAAVGAYKSINKNWTFAGVLGVLSAFFIMGF
                     YPVVGGWALAYVLKSFTGLLSNAAVIGDAFGAFIGNPMEPVIWMLIFLAMNIAIVAKG
                     IAGGIEKAGKILMPTLFILLVLVAARSMTLPGAGAGIDFLFKPDFSAVSGATFLAALG
                     QAFFSLSLGMGCMITYGSYLGKSENLPQNALIISMLDTGVALLAGLAIFPALFAFGME
                     PAVGAGLVFVVVPQIFAAMGGIGTVFSAIFFVALTVAALTSSVSLLEVVVAYLIDQRG
                     WERKKSVYTAGGIMVVTGILSSLSMGLMSGFTILGVGIFDFFDILTDKIFLAIGGLLL
                     AIFVGWFMNKQDLEDELTNGGTIKFGLFNIWYNLIKYVIPVAIAIVAFAGITSIAQKG
                     LMFLGLGIIVVLAIFSKKL"
     gene            complement(1968..2618)
                     /locus_tag="VFD79_04365"
     CDS             complement(1968..2618)
                     /locus_tag="VFD79_04365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZH93314.1"
                     /translation="MKFLNEEVAKQLEGIFNGMKDDVTVALFTRAQDCETCEDTKDFL
                     TELTSVAPRLSLEILDLDKDSDKAEEFGVTMAPSLVLLDKDKTYQRIKFNGVPSGHEI
                     NSFISSVMEVSGNAQELPKEIMERIEKIQKPVNIKVFVTLACPHCPGAVEKAHKLALE
                     NPNIQADMIEAQTFYDMSEEYNVSSVPKIVINDEFEFVGNQPLEVFLDEIEKTQVA"
BASE COUNT          751 a          504 c          540 g          863 t
ORIGIN      
        1 caagagattt aacctttcta tgcttggttt tcccagttga aggctcccta taaccctcaa
       61 caatagatag atatattctg ccattagact ttttatcctt tttcaaaaac atgtcaacca
      121 cctcatatac attataccat aatacaacta ggacaacaag aaaaagatta aaatttgaca
      181 aaaaaatttc cttgtatcaa ttgatatcac aaggattaga gtctaagttt tcaattattt
      241 gctgcaaaac tcgcgaagct ggtggcctgt atagaaagct aggcttcaca caatttgata
      301 ccgttatgga atacaataga atcttgttat aagaacatct gtccatggtg cagatgctct
      361 taacttcaca ttgttaagaa attaactaat atttcgcaat tatctgtaaa ttcaactaga
      421 caaataaact cttttgtatt attatattta ttaagatgtg aaataattgc atcattacag
      481 tactaaagga gggaatattt gatggaaaag aaacaaagag aaaattgggg atcccggatt
      541 ggattcatca tggcagcggc tggttcagcg gttggacttg gtaacatttg gagatttccg
      601 taccttgcag gtgaaaacgg aggtagtgca ttcattttaa tttatcttgg cttcgtactg
      661 gtaatcggac ttagtatcat ggtatccgag ttggcagttg gacgaagaac tgaacttgca
      721 gcggtaggag catacaaatc cattaacaag aactggacat ttgctggtgt tcttggagta
      781 ttaagtgcat tctttataat gggcttttat ccagtagttg gtggatgggc attggcttat
      841 gtacttaagt catttacagg acttctttca aatgctgcag tcataggcga tgctttcgga
      901 gcatttattg gtaatcctat ggagccggtt atctggatgc tgatattcct agcaatgaac
      961 attgctatcg ttgcaaaggg tattgcaggc ggtattgaaa aagctggtaa gattcttatg
     1021 ccaacactgt ttattttgtt ggtacttgta gcagcaagaa gtatgacgtt accaggagct
     1081 ggagcaggaa ttgatttcct atttaagcct gattttagtg cagtttcagg agcaacattc
     1141 cttgcagcct tgggccaggc attcttctca ctatcacttg gtatgggctg tatgatcacc
     1201 tacggtagtt atcttggaaa atcggaaaac ctaccacaaa atgcactaat aatttctatg
     1261 cttgacacag gtgttgcact tctagcagga ttggcaatat tcccagcgct ttttgcattt
     1321 ggaatggagc cagctgttgg agccggacta gtattcgtag tagtaccaca gatattcgca
     1381 gcaatgggag gaatcggaac tgtattctca gcaatattct ttgtggcact tacagtagct
     1441 gcacttactt catcagtatc actgcttgag gtagttgttg cctacctgat cgaccaaaga
     1501 ggctgggaaa gaaagaaatc tgtttataca gctggtggaa taatggttgt aactggaatc
     1561 ctttcatcac tttcaatggg attaatgtca ggatttacaa tccttggagt aggaatcttc
     1621 gacttcttcg acattcttac tgacaagata ttccttgcta tcggtggatt gcttcttgca
     1681 atattcgttg gttggttcat gaacaagcaa gaccttgagg atgagcttac aaacggagga
     1741 acaatcaagt ttggtctatt caatatttgg tacaacctaa ttaaatatgt gattccagta
     1801 gctatcgcaa tagttgcttt tgcaggaatt acatcaatag cacaaaaagg acttatgttc
     1861 ttaggtcttg gtatcatagt tgtattggca atattttcaa agaaactata acaataaaaa
     1921 taaaaaatca tgtttcccac ttgggaaaca tgattttttt tgcccttcta tgctacttgt
     1981 gttttttcaa tttcatctag gaatacctca agtggctggt ttcctacaaa ctcaaactca
     2041 tcgtttatta caatctttgg tacacttgat acattgtatt cttcagacat gtcatagaag
     2101 gtctgggcct cgatcatatc tgcctgaatg ttcggattct caagtgccag cttgtgggcc
     2161 ttctcaacag ctcctggaca gtggggacat gccagggtga caaatacctt tatatttact
     2221 ggtttttgta tcttttcaat cctttccatt atctcctttg gaagttcctg ggcattacct
     2281 gagacctcca taacactgga aataaaggag ttgatttcat gacctgacgg aaccccgttg
     2341 aacttgattc tctggtaggt cttgtccttg tcaaggagta caagactagg tgccattgta
     2401 accccaaact cttcagcctt gtctgaatcc ttgtcaagat ccaggatttc caagcttagc
     2461 cttggtgcaa ctgatgtaag ctccgtaagg aaatctttgg tatcctcaca ggtttcacag
     2521 tcctgggccc tggtgaaaag ggccaccgtt acatcatcct tcattccgtt aaagatacct
     2581 tcaagctgct ttgctacttc ctcgtttaaa aatttcataa ttacctcctg tggtttactt
     2641 gtttatagtg tttaaata
//
LOCUS       DASRQF010000159         2088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1244793, whole genome shotgun sequence.
ACCESSION   DASRQF010000159 DASRQF010000000
VERSION     DASRQF010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244793"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1525
                     /locus_tag="VFD79_04370"
     CDS             <1..1525
                     /locus_tag="VFD79_04370"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HZH93315.1"
                     /translation="AGTRLTTSNRLSVGGKSPLTGGIKESNSGGYAAALFADHGLKLI
                     AVKNIPREDKFWILRITSDGEAGLEDAEEYRGMNNYDFVDAIKERFGDKLATVSIGTA
                     GERKYKASSIQISVFNSGHPSRAAARGGLGALMGSKGLKAIVVEKPVEKFRFEFKDED
                     KFNNLALKLNKMVAEGAKTDPFHNIGTIATIEATGENGILPVKNFSGKLFPDYKEVGA
                     KKFMTNLATRGGKNKMACQPGCVLQCSNCYNDKDGNYLTSGFEYETIALFGPNCGITD
                     LDAIASMDRLCDDIGVDTIDTANAIAMCMETGKLQWGDAVGAMDLLKEMADGEGFGQI
                     MGNGCEAVGKELNSERIPVVKHQAMAAYDPRNTKGTGITYATSPMGADHTAGLTMGRA
                     FDDRGRAAQSYVSNKLQVAMSFADSMMCIFAFAHVVPALPLLGEMMGAMYGGSSEYSR
                     VTTMGVKTLLTERGFNKMAGMTEKDDRLPEFFYKERSEATGSRFDIDDYEMDVLFEF"
     gene            1525..1722
                     /locus_tag="VFD79_04375"
     CDS             1525..1722
                     /locus_tag="VFD79_04375"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HZH93316.1"
                     /translation="MIRVNDREVEFKRGITVADAIKSSGWPTGEGIIVMVDGEVLSKE
                     ELSREAESDTEIRVMLLVSGG"
     gene            1795..>2088
                     /locus_tag="VFD79_04380"
     CDS             1795..>2088
                     /locus_tag="VFD79_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZH93317.1"
                     /translation="MLIYSYEGLIRADRRSDIVGIRIFHTGDIHIGMTFNSYPAGIRE
                     LLKAARIEVIERMVKQANREGSDLFVVAGDLFDKIKGIDKKSQAAVAEHLSKFE"
BASE COUNT          635 a          368 c          604 g          481 t
ORIGIN      
        1 tgcaggcacc aggctaacaa cctccaaccg cctttcggtt ggagggaaaa gcccattgac
       61 tggaggaata aaggaaagca actcaggggg ctacgcagct gcattgtttg cagatcatgg
      121 cttgaagctt attgcagtta aaaatattcc aagggaggat aagttttgga tactcaggat
      181 caccagcgat ggagaggctg gacttgaaga tgctgaggag tataggggca tgaacaatta
      241 tgattttgtg gatgccataa aggagaggtt tggagataag ctggccactg tttcaatagg
      301 tacagcagga gaaagaaagt ataaggcctc atcaatacag atctctgtgt ttaattcagg
      361 acacccaagt cgagctgctg caaggggagg actgggagct ctgatgggaa gcaagggact
      421 aaaggcaatt gtggttgaaa agccagttga aaagttcagg tttgaattca aggatgaaga
      481 caaattcaac aatctagcat taaaactgaa taagatggtg gcagagggtg ccaagacaga
      541 tcccttccac aacattggaa caatagcaac cattgaagct acaggtgaaa acggcatact
      601 tccggtgaaa aacttctcag gaaagctttt ccctgactac aaggaggtcg gggccaagaa
      661 attcatgaca aacctggcaa ccaggggcgg aaagaataag atggcttgcc agcctgggtg
      721 tgtcctacaa tgctcaaact gctacaatga caaggatggc aactacctta catccggctt
      781 cgagtacgag accatcgccc tgtttggtcc aaactgtgga atcacagacc ttgatgccat
      841 agcaagtatg gatagattat gcgatgacat tggggttgat accatagata cggcaaatgc
      901 catagccatg tgtatggaga caggtaaact acagtgggga gatgcagtgg gagccatgga
      961 tttgcttaag gaaatggctg atggagaagg attcggacaa attatgggga atggctgtga
     1021 agctgttgga aaggagctta acagcgaaag aataccggtg gtaaagcacc aggcaatggc
     1081 tgcatatgat cccaggaata caaagggaac tggaatcacc tatgccacaa gtccaatggg
     1141 agctgaccac acggcaggtc tgacaatggg aagggctttc gatgacaggg gaagagccgc
     1201 ccagtcctat gtatccaaca agctccaggt agccatgagc tttgctgaca gcatgatgtg
     1261 catatttgca tttgcacacg tggtccctgc attgcccctg ctgggagaga tgatgggagc
     1321 catgtatggg ggttcatcgg aatactcccg tgtaaccaca atgggtgtga aaaccctgct
     1381 gacagaaaga ggcttcaaca agatggctgg aatgactgaa aaggatgaca gattacctga
     1441 attcttctat aaggaaaggt ctgaggcaac cggatctcga tttgatattg acgattatga
     1501 gatggatgta ctttttgaat tctaatgatt agggtcaatg atagggaagt ggaattcaag
     1561 aggggaatta cggttgcaga tgccattaag agttcaggct ggcctacagg agaaggaatc
     1621 atagtcatgg ttgatggaga ggttttgtca aaggaggagc tttccaggga agctgagagt
     1681 gataccgaaa taagggtgat gcttctggtt tcaggaggat aggctcgatc aaatttcagg
     1741 tttaaaggga catccgcaag ggtgtccttt ttgttgggaa aaaggtgcca ggttatgtta
     1801 atatattctt atgaaggatt gataagggct gataggagga gtgatattgt ggggatcagg
     1861 attttccata ctggagacat acatataggc atgaccttta acagctatcc tgcaggtata
     1921 agggagcttt tgaaggctgc acggattgaa gtgatagaaa ggatggtaaa gcaggccaac
     1981 agggagggaa gcgacctgtt tgtagtggca ggggaccttt ttgacaagat caagggtatt
     2041 gataagaaga gccaggctgc ggtggcagag catctgtcga aatttgaa
//
LOCUS       DASRQF010000160         3791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_5003, whole genome shotgun sequence.
ACCESSION   DASRQF010000160 DASRQF010000000
VERSION     DASRQF010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5003"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /gene="ilvN"
                     /locus_tag="VFD79_04385"
     CDS             <1..248
                     /gene="ilvN"
                     /locus_tag="VFD79_04385"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HZH93318.1"
                     /translation="TRKRSILRELMFLKISCSKEGRSEILQIANVFKGKIVDIAAKSV
                     TIEITGDEDKNNAFLELVKPYGILEIVRTGLTAIERG"
     gene            complement(315..1832)
                     /locus_tag="VFD79_04390"
     CDS             complement(315..1832)
                     /locus_tag="VFD79_04390"
                     /inference="COORDINATES: protein motif:HMM:NF021619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZH93319.1"
                     /translation="MRQEKHFHLGEEQLFFVKKGNGCFYSDGIEEKINPSKIIYIKPF
                     SEHEVINTGDEDLVLHAVYVPIKLMQLERHFSLPVDSNIQDIIPMEILENIENQLSDL
                     LKMQIHILDRDYTRLFENNGENEFCQLCLSENRCTMSQYGFEGSGQNLDTVYRCKFNL
                     FELKASITVNDNILGYIRSERFVLDDSDKNKKNILDFSRVINIDMDSLLEAYKKVPYI
                     IKSRTYVIHEHLAIAAEFIQDMLHRSIMEKELIDKDNEILTSTIEKIQLKDALKKANH
                     RIYNDRIFTGESRIRNEVAYPYELEMLLEEAIQKLDKKGIDKLIEEHRKKYPIGKGIL
                     SEMIVVLSRTALRPLENVELTAKIRNRYERYLQGEVKEEPWKILKEFSLECIGEYIAI
                     SQTNGGSLIDNINMFIKSHYKEDLSLNLVAERFFISPNYLSSIFNERNGVSFSEYIHT
                     LRIDEAKLLLKNLNIRVHEISSRVGYKNNSYFSSIFKRYVGLTPNEYRKSLAKDN"
     gene            complement(2071..3042)
                     /locus_tag="VFD79_04395"
     CDS             complement(2071..3042)
                     /locus_tag="VFD79_04395"
                     /inference="COORDINATES: protein motif:HMM:NF024981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HZH93320.1"
                     /translation="MKNVAKLISKYFGIIIILFMLVAFIEPGIFSWVTSQLFGQSVIT
                     LLLGIIMFGMGMTLNIEDFKIVLKRPLDILKGTLAQYTIMPLLALALSMMFGLEEALM
                     VGVVLVGTCPGGTSSNVISYMAGGDVALSVAMTTVSTLLAPILTPAITYMLIRQSVTF
                     SPIGMFTSIVQVVILPIALGLLLKSIIKDKADVLEDYLPAVSSIAIACIVGGVIGGNR
                     ERILASLGLIVVVVVLHNILGYLIGLGIGRLTGMDRRKSITIAVEVGMQNSGLATSLA
                     ASQFAAMPLAAVPGALFSAWHNVSGAVFAWIAKSIESREPNPQVNSN"
     gene            complement(3056..>3791)
                     /locus_tag="VFD79_04400"
     CDS             complement(3056..>3791)
                     /locus_tag="VFD79_04400"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HZH93321.1"
                     /translation="DLDDAGGVYAVMNELVKKDLLRTDIMTCTGRTVAENISGCYNQN
                     PDVIRPVEDPYSETGGIAVLKGNLAPDTCVVKRSAVLPQMMKHEGPARVFDCEEDAID
                     AIKGGGIVPGDVVVIRYEGPKGGPGMREMLNPTSAIAGMGLDSTVALITDGRFSGASR
                     GASIGHVSPEAAVGGNIALVEDGDIIRIDIDANTIDMDVSADELEKRRKAWTPREPKI
                     TTGYLSRYASMVTSGNRGAILEVPKK"
BASE COUNT         1062 a          836 c          702 g         1191 t
ORIGIN      
        1 tcacaagaaa aagatccata ttaagagagt tgatgtttct gaaaatatcc tgcagcaagg
       61 aaggcaggtc tgagattctt cagattgcca atgtattcaa gggcaagata gtggatattg
      121 ctgcaaaaag tgtgaccatt gaaattacag gagatgaaga caaaaacaat gcatttttgg
      181 agcttgtgaa gccctacgga attcttgaga tagttcgaac cggcttgacg gcaatagaaa
      241 gaggatagat aattgattga actaaagaag ctccgccttt tggcgagagc ctctttattc
      301 aaagaccagc aggtctaatt gtcttttgcc aaagactttc tatattcgtt tggtgttaaa
      361 ccaacatatc tcttaaagat acttgaaaag taactgttgt ttttgtatcc aactcttgag
      421 gatatttcat ggacccttat gttcaagttc tttaaaagca atttggcctc atcgattcgc
      481 aatgtgtgga tatactcgct aaaggaaaca ccattcctct cgttgaaaat agaacttaag
      541 taatttgggc ttataaaaaa ccgctcagca acaagattta gacttaggtc ttctttatag
      601 tgggatttaa tgaacatgtt tatattgtct atgagactac ccccgtttgt ttgggatatg
      661 gctatgtatt ctccaatgca ctccaatgaa aattctttaa ggattttcca gggttcttcc
      721 ttaacctctc cttgtagata tctctcgtag cggttcctta tcttcgctgt cagttcaaca
      781 ttttccaaag gtcttaaagc agttcttgat aatacgacaa tcatttcact taggattccc
      841 ttgcctattg gatatttctt tctatgctct tctatcagtt tgtctatgcc ttttttatca
      901 agtttctgaa tggcttcttc aagtagcatt tcaagctcat aaggatatgc aacctcattt
      961 ctaattcttg actcgccggt aaagatccta tcgttgtaga tacgatgatt cgccttcttt
     1021 aaagcatctt ttaattggat tttttcgatt gtgcttgtaa ggatctcatt atccttatca
     1081 atgagctcct tctccattat gcttctgtgg agcatatcct gaataaattc agccgcgata
     1141 gccaagtgtt catgtatgac ataggttcta ctttttatta tgtagggtac ttttttgtat
     1201 gcttccaaaa gtgagtccat gtcaatattg atgaccctgc tgaaatcaag gatatttttc
     1261 ttgtttttgt cggaatcatc aaggacgaaa cgctcacttc ttatatatcc aagaatatta
     1321 tcgtttacag tgatggaagc tttcagttca aataaattaa acttgcatct atagactgta
     1381 tcaaggttct gtccggatcc ttcaaagcca tattggctca tggtgcatct gttttctgat
     1441 aggcaaagct ggcagaattc attttcaccg ttgttttcaa aaagtctggt gtaatctcta
     1501 tccaatatgt gaatttgcat ttttaacagg tcagagagct gattttcaat attctcaaga
     1561 atttccatgg gtattatgtc ctggatgtta ctatcaaccg gcagagaaaa gtggcgctca
     1621 agttgcatca gttttatcgg aacatacacc gcatgaagaa ccaggtcctc atctccagtg
     1681 tttatgactt catgctctga aaagggcttg atatatatta tcttggaggg atttattttt
     1741 tcctctattc cgtcagaata gaagcatcca tttcccttct ttacaaaaaa aagttgctct
     1801 tcccctaaat ggaaatgctt ctcctgtctc aaaccaggat aaattattat ttgtgctaca
     1861 ctaattctaa ggtatggaga ttcaactggc tcatgtagcc acaggatttc tccccaatca
     1921 aaaatctgct tgtcatattt atcaatatta tccagtagat tcataacata tcccccacaa
     1981 attgtaaaaa tctttataca tataatatag ttaacaataa aaaaaagcaa acccttttgg
     2041 atttgcttta gagtataaga ttttaaattc ctagtttgaa ttaacctggg gatttggttc
     2101 cctggattct atggacttgg caatccaggc aaatacggca cctgatacat tatgccatgc
     2161 actgaagagt gctcctggaa ctgcagccaa gggcattgct gcgaattggc ttgcagcaag
     2221 ggaagtggcc aaaccggaat tctgcattcc aacctcaaca gcaattgtga tactctttct
     2281 gcgatccata cccgtcaacc ttccaatgcc aagtcctatt aggtatccca gaatattatg
     2341 aagcacaacc acaacaacaa taagtccaag ggatgccagg atcctttccc tgtttccacc
     2401 tattactcca cctactatgc aagctatggc aattgatgaa acagcaggaa gatagtcctc
     2461 caaaacatca gccttgtcct ttattatgga ttttaggagc aggcccaggg cgatagggag
     2521 gataaccacc tgtacaatgc tggtaaacat tcctattgga ctgaaggtta ctgattgtct
     2581 aatcaacata tatgtaatcg ccggtgtaag aattggagca agcaaggtgg agacggttgt
     2641 cattgccaca gatagggcaa catcccctcc agccatatat gatattacat ttgaggatgt
     2701 acctcctggg caggttccca caagaacaac accaaccata agcgcctctt caagaccaaa
     2761 catcatggat agtgccagtg ccagcaaagg cattatggtg tattgtgcta aggtgccctt
     2821 tagaatatcc aatggtcttt tcagtactat cttgaaatcc tcaatattca acgtcattcc
     2881 cattccaaac attataatcc ccagtagcaa tgttattaca gattggccga acagttgtga
     2941 tgttacccaa ctgaatatgc cgggctcaat aaaagccacc aacataaaca aaattattat
     3001 tatcccaaaa tattttgaga taagctttgc gacatttttc actcaaatac cccctttatt
     3061 tttttggaac ttcaagtatt gcgcccctgt tgccggatgt taccatggat gcatatcggg
     3121 aaaggtaacc ggttgttatc ttaggttctc ttggagtcca tgcttttctt ctcttttcaa
     3181 gctcatccgc tgaaacatcc atatctattg tattggcatc gatatctatc ctaatgatgt
     3241 cgccatcttc caccagggct atatttccac ctacagcagc ttcgggagaa acatgtccga
     3301 ttgaagcacc tcttgaagcc ccgctgaatc gtccgtcggt tataagggca accgtactgt
     3361 caagacccat tccagctatt gcagaggttg gatttagcat ctccctcatg cctgggccac
     3421 ccttgggccc ttcatacctt attactacaa catcaccagg gacaattcca ccccccttga
     3481 tggcatcaat ggcatcctct tcgcagtcaa aaacccttgc agggccttca tgcttcatca
     3541 tttgtggcag tacagccgat cgcttcacta cacacgtatc cggtgccagg ttacccttta
     3601 ggacagcaat tccaccggtt tcactgtagg gatcctcaac tggtctgatt acatctggat
     3661 tttggttgta gcatccggaa atgttttctg ccacagtcct gcctgtgcag gtcataatgt
     3721 cagttcttag aaggtccttc ttgacaagtt cattcatcac agcataaact ccccctgcat
     3781 catccaggtc c
//
LOCUS       DASRQF010000161         7637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_523604, whole genome shotgun sequence.
ACCESSION   DASRQF010000161 DASRQF010000000
VERSION     DASRQF010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 7637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7637
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523604"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VFD79_04405"
     CDS             <1..890
                     /locus_tag="VFD79_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZH93322.1"
                     /translation="TEFLDGFTHEEVEDLAMDKDELLENLEVFINQMKEFLGIKEEVG
                     VRSLTILPGFDKSGQPEGFSELTIETSQVISIVGPTGSGKSRLLADIEWTAQNDTPTG
                     RSILINDELPDKKWRFSSNNKLVAQLSQNMNFVMDLTVMEFLQLHAESRLVEDIEGTI
                     ERIIKAANSLAGEKFQLDTPVTALSGGQSRALMIADTAILSSSPIVLIDEIENAGIDR
                     RKALNLLIDEDKIVLMATHDPTLALIADKRIIIRNGGIAKIIETTEEEKEILHKLEKM
                     DTYIQEMRSNLRNGESLTI"
     gene            934..1176
                     /locus_tag="VFD79_04410"
     CDS             934..1176
                     /locus_tag="VFD79_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93323.1"
                     /translation="MHDGCTGSFGSGKAVVDKIRAMGFNTRPMPPGVTINCIECGSDF
                     EMTHLETKCPSCEMVYGVTPCSTAYPERIKAAGKNY"
     gene            complement(1173..1892)
                     /locus_tag="VFD79_04415"
     CDS             complement(1173..1892)
                     /locus_tag="VFD79_04415"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZH93324.1"
                     /translation="MFQTHMTEVITKFNIATGIPISLYSAELELLDQRGLSKSDELSM
                     EEFTMGKLSRLIGNGNHESYIPLDMKLYLVCVPVEGDKSNGYYALGPSTCSKGVYSWA
                     RYLPRALMPNLIALLRSFENDYSLTGVQRCQGFSLHVNRADHYISANYHEKITLDDIS
                     TYLGLDRTYFSSLYKKETGKSFTQSLNDHRIEKSKSLLLDGNSSIMDIALAVGFSNQN
                     YFNSVFKSITGLTPSEFRNGN"
     gene            2058..3002
                     /locus_tag="VFD79_04420"
     CDS             2058..3002
                     /locus_tag="VFD79_04420"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZH93325.1"
                     /translation="MNKFIVLLLLVMLSLASLFIGAGDITAMDILRLEDSKVELLLIS
                     RIPRLASIIIAGMGMSIAGLIMQQISRNKFVSPTTAATVDSSRLGILVGMILIPGAGT
                     LWKMSLSFVFAVGGTMVFMRLLDRIKIKNTVFIPLVGIMLGSIIESFTTFLAYRYDLV
                     QNVNSWLQGDFSMIMKGRFELLYISIPLLIVAWKYADRFTVAGMGEDFALNLGLNYKT
                     VVNIGVTIVALISATVIVTVGRIPFLGLIIPNLVTLYRGDNMKGNIWIVALSGSAFLL
                     FADILGRLVIYPYEVSISLTVGVMGSMIFLYLLLRGDR"
     gene            2999..3952
                     /locus_tag="VFD79_04425"
     CDS             2999..3952
                     /locus_tag="VFD79_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HZH93326.1"
                     /translation="MMDNKRKIVILTLISLAMTLAFVFWGLGPTNWRFFIQTRLPKVA
                     AIAITGAAIGYSSLLFQTVSNNRILTPSVLGLDSLYMLLQTFVIFTFGSTSRVAVDRN
                     LNFFITVILMVLFSTLLFKLLLQRESGNIMKLLLVGIIMGTLFSSLSSFMQMVIDPNE
                     FMHVQDRMFASFNRVNTDILIVAMVITIGALSLTLKDTALWDVLTLGRDNAINLGVDY
                     DRTIRRMLFTVSLLVSVSTALVGPITFLGMLVVNLAREMLNTFRHRELITGSILLGIL
                     FLVGGQLVVERVLNFSTPISVIINFVGGIYFIKLLLKESVG"
     gene            3949..4704
                     /locus_tag="VFD79_04430"
     CDS             3949..4704
                     /locus_tag="VFD79_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZH93327.1"
                     /translation="MIEIRGIEKNYGKLRVLDDVSTTIEKGKITSFIGPNGAGKSTLL
                     SVVSRLVNQDRGQVLIEGRSLTDWDSDELAKKVSILRQSNHMNVRLTIRELVSFGRFP
                     HSKGRLTKEDKDFIDRAIEYMKLEDIQHKYLDQLSGGQRQRAYIAMVIAQDTDYILLD
                     EPLNNLDMKHAVEIMRMLKKLVRDLDKTIAIVIHDINFASCYSDRVVAMKDGRLMEEG
                     ETDKIINSHSLGQIYDMDFQVTRMNNMNLCVYF"
     gene            4731..5672
                     /locus_tag="VFD79_04435"
     CDS             4731..5672
                     /locus_tag="VFD79_04435"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HZH93328.1"
                     /translation="MKNVKSMFLSIMAVVLAIGAMGCSASPEAEGATGVKTVTISHEL
                     GDTTVALNPERVVVFDYGILDSMKELGIEVIALPKQSLPAHLGEFGGMEYIDAGTLKE
                     PNFEVIYEASPDLIIVSGRQVDMYDDFARIAPTVYMPMDNGEYLESLRNNLNILGQIF
                     DKKEMVDEKVDDIESSIQYLNEIASDRVENGLFVMANDGNLSVYGEGSRFGVLHEEFG
                     IPAADREIESSTHGQKITFEYIQQIDPEYIFVMDRAAVAGGEVSAKSVMDNEIIGMTR
                     AYAEGKIIYLDAHVWYVGAGGIGSTEKMINEIISAIE"
     gene            5825..7498
                     /gene="hcp"
                     /locus_tag="VFD79_04440"
                     /gene_synonym="priS"
     CDS             5825..7498
                     /gene="hcp"
                     /locus_tag="VFD79_04440"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004821215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HZH93329.1"
                     /translation="MAENQMFCYQCQEAAGGKGCTKVGMCGKTSEVANLQDLMIYGLK
                     GLSELGVKADEIGYKMEGLDRLIIEGLFMTITNANFDKDRFFEGIKLVLKKRDELKSI
                     LEEKGVDLQGLKDAATFTVNSNDEIVFKSNSAEVSVLATENEDVRSLRELIIYGLKGM
                     AAYAEHADNLGFEDEEISKFIRKALVATMDDSMSADDLVALTLETGKYGVQTMALLDK
                     ANTSTYGNPEISEVNIGVRNNPAILVSGHDLKDFEELLEQTKDSGVDIYTHGEMLPAN
                     YYPTFKKYDHFVGNYGNAWWQQKEEFDKFNGAILFTTNCIVPPKADYADRVYTTGSSG
                     YPGFKHIPDRVDGQPKDFSEVIEHAKRLPAPTEIETGKIVGGFAHAQVFALADKVVEA
                     VKTGAIRKFFVMAGCDGRQGKRSYYTEFAEALPKDTVILTAGCAKFRYNKLNLGDING
                     IPRVLDAGQCNDSYSLAVIAMKLKEIFELNDINDLPIAYNIAWYEQKAVIVLLALLHL
                     GVKNIHLGPTLPAFLSPNVAKVLVDTFGIGTISDVENDINMFLGEAANA"
BASE COUNT         2362 a         1373 c         1845 g         2057 t
ORIGIN      
        1 ttacagaatt cctggatgga ttcacccatg aagaggttga agacctggcc atggacaagg
       61 atgagctttt ggagaatctt gaagttttca taaaccagat gaaggaattt ctgggaataa
      121 aggaagaggt tggggtaagg tcactgacaa tactccctgg gtttgacaaa tccggacagc
      181 ctgaaggctt cagtgaactt acaatcgaga catcacaggt aatatcaata gttggtccaa
      241 ctggatcagg aaagtcaaga ttgctggctg acattgagtg gactgcacag aatgataccc
      301 caactggaag gagcatcctg ataaacgatg agcttccaga caaaaaatgg agattttcat
      361 ccaacaataa gctggttgca cagctttccc agaacatgaa ctttgtcatg gaccttactg
      421 tgatggaatt tcttcagctt cacgctgaaa gcaggttggt ggaggacatt gaaggtacaa
      481 tagaaagaat aatcaaggca gcgaacagcc tggcagggga gaaatttcag ctggacactc
      541 ctgttacagc attgagtgga ggtcaatcta gggcgcttat gattgcagat acggccatac
      601 ttagttcctc gcctatagtg ctgattgatg aaatcgagaa tgccggcata gacaggagaa
      661 aggcccttaa cctgctaata gacgaggaca agatagtgct tatggcaacc catgacccaa
      721 cccttgccct tattgcagac aagaggataa tcataagaaa cggtggaata gcaaaaatca
      781 tcgaaacaac agaagaggaa aaggagattc tccacaagct tgaaaagatg gatacctaca
      841 tccaggaaat gagatcaaat ctaaggaatg gagaatcttt aaccatatag tggaaaggct
      901 ggaacccaag aaaaaaatag gaggtataat attatgcatg acggatgtac aggaagtttt
      961 ggaagtggta aggcagtggt ggacaagatt agggcgatgg gctttaacac tagacccatg
     1021 ccaccaggag ttacaattaa ttgcatcgag tgtggcagcg actttgaaat gacccaccta
     1081 gaaacaaaat gcccaagctg tgaaatggtg tatggagtga caccatgttc aacagcatat
     1141 cctgaaagga tcaaggcagc tgggaagaat tattaattcc catttctgaa ttctgatggt
     1201 gttaagccag ttatactttt aaaaactgaa ttaaagtagt tttgatttga aaaacctacg
     1261 gcaagagcta tatccataat ggaggaattt ccatccagga gaaggctctt gcttttttca
     1321 attctatgat cattgagaga ctgtgtgaag gacttgccag tctctttttt gtatagactt
     1381 gagaaatatg tcctgtctaa gccaagatag gttgaaatat catccaatgt gattttttca
     1441 tggtaatttg cgcttatgta atggtctgcc ctgtttacat gaaggctaaa tccctggcac
     1501 ctttgtactc ctgtaagcga atagtcgttc tcaaagcttc gcaacagagc aatgagatta
     1561 ggcatcaaag ccctgggcag gtacctggcc caggagtaaa cccccttgga acaggtggaa
     1621 ggccctagtg catagtagcc attactctta tcaccctcca ctggtacaca taccagataa
     1681 agcttcatat ccaaaggtat gtatgattca tggttcccat ttcctatcag cctggatagt
     1741 ttgcccatag tgaattcctc catcgacaac tcatcacttt ttgaaaggcc cctttgatct
     1801 agaagttcca attctgctga gtacagggat attggaattc cagttgcaat attgaattta
     1861 gttatgactt cagtcatatg tgtttggaac attattgtgt acctcctgaa ttatgtgaaa
     1921 tattttgtaa gaaatccaaa gattgtttaa acctaaggtg gattgccatg gtatattaat
     1981 gagagtgatt atcaatatca acaatatagc atatatggtc acaagcttca accactttac
     2041 atagaacgga tgatatgatg aataagttta ttgttttact cctattggta atgctatctt
     2101 tggcatcact atttattgga gctggggata ttacagcaat ggatatcttg cgtctggagg
     2161 actccaaggt ggagcttctt ctgataagca ggattccaag attggcaagt atcataatag
     2221 ctgggatggg catgagcata gcaggactta tcatgcagca gataagcagg aataagtttg
     2281 tatcaccaac cactgcagcc actgtggact catcaagatt ggggatactg gtaggtatga
     2341 ttctcattcc aggagcaggc acattgtgga agatgagttt atcttttgtg tttgcggttg
     2401 gagggacaat ggtttttatg agacttcttg ataggattaa gatcaagaat actgttttca
     2461 ttccattggt ggggataatg ctaggtagca taatagaatc ctttacaacc tttcttgcat
     2521 acagatacga cctggtgcag aatgtgaatt catggctaca gggagacttt tcaatgatta
     2581 tgaagggtcg gtttgagctc ttgtacataa gcataccttt attgatagtg gcatggaaat
     2641 atgccgacag gttcactgtt gcgggtatgg gtgaggactt tgcattgaat ttgggcttga
     2701 actacaagac agttgtgaat attggtgtca caattgttgc cctgatatcg gcaactgtaa
     2761 tcgtaacagt aggaagaatt ccttttctgg gattgattat accaaacctg gtgacccttt
     2821 acagggggga taacatgaag ggtaacatct ggattgttgc cttgtcaggc agtgcttttc
     2881 tcctgtttgc ggatattctt ggaagactgg tcatttatcc ctacgaggtt tccataagcc
     2941 tgactgtggg agtgatgggt agtatgatct tcctctacct gctattgagg ggggatagat
     3001 gatggacaat aagagaaaga ttgtaatact tacactgatt tctttagcga tgacattggc
     3061 atttgtgttc tggggactag gtccaacaaa ttggaggttt ttcatccaga caaggttacc
     3121 caaggttgca gccattgcaa ttacaggagc tgcaataggc tattcatccc tgcttttcca
     3181 gacagtcagc aacaacagga tattgacccc aagtgttctg gggcttgaca gcctgtatat
     3241 gctactccaa acctttgtaa tattcacatt tggatccaca agcagggtag cagttgatag
     3301 gaatctgaac ttttttatca ccgttatact tatggttctg ttctcgacac tgcttttcaa
     3361 gctcctcctc caaagggagt caggcaatat tatgaaactg ctgcttgtgg gaataataat
     3421 gggtacactt ttttcaagtc tttcgtcctt tatgcagatg gtgatagacc caaacgagtt
     3481 catgcacgtc caggacagga tgtttgccag cttcaacagg gtaaatacag acattctgat
     3541 tgtagccatg gttataacca taggagcatt gtccttgaca ttgaaggata cagccctttg
     3601 ggatgtgctg acactgggca gggataatgc cataaacctt ggtgttgact acgacaggac
     3661 tataaggagg atgctattta cagtatcatt acttgtctct gtatcaactg ccctggttgg
     3721 acctatcaca tttctgggga tgctggttgt gaacctggca agggagatgc tgaacacctt
     3781 taggcacagg gaactcatta ctggttcaat actattggga atactgtttc ttgtcggggg
     3841 ccagcttgtg gttgaaaggg ttcttaactt ttcaacacca atcagtgtaa taataaattt
     3901 tgtgggagga atttacttta ttaaactttt attaaaggag agtgtgggat gattgaaata
     3961 agaggaattg aaaagaacta cggtaaattg agggttctag acgatgtaag tactacaatc
     4021 gagaagggta agataacatc ctttatcgga ccaaatggtg ccggaaagag caccctgctt
     4081 tcggttgtaa gcagacttgt gaatcaggat aggggccagg tcctgattga aggaaggagt
     4141 ctgacggatt gggatagtga cgagctggca aaaaaggttt ccatactaag acagtcaaac
     4201 catatgaatg taagactgac catccgtgaa cttgtaagct ttggaagatt cccccacagc
     4261 aagggaagat tgacaaaaga ggataaggat ttcattgaca gggcaattga gtacatgaag
     4321 cttgaagata tccaacacaa gtatctggat cagcttagtg gtggtcagag gcaacgggca
     4381 tacatagcaa tggttattgc ccaggacact gactacatac ttcttgatga gccactgaac
     4441 aacctggata tgaagcatgc cgttgaaata atgagaatgc tgaaaaagct ggtaagggat
     4501 ttggataaga ccatagcgat agtaatacac gacatcaact ttgcatcctg ttactcggac
     4561 cgtgttgtgg ctatgaagga tgggagactg atggaggaag gggaaacaga caagataatc
     4621 aattcccatt cccttggaca gatttatgat atggattttc aagtgacacg aatgaacaat
     4681 atgaatcttt gcgtatattt ctaacttaac aattaaggag tgaatgatta atgaaaaatg
     4741 ttaaaagtat gtttttatca attatggcgg tggtccttgc aattggagca atgggctgtt
     4801 cagcatcccc tgaggctgaa ggtgcaactg gtgtcaagac cgtaacaata agtcatgagc
     4861 tgggagatac aactgttgcc ttgaatcctg aaagggtggt tgtgtttgat tatgggatcc
     4921 tggactcaat gaaggaactg ggaatagaag taatagccct tccaaagcaa tcgctgcctg
     4981 cccatctagg tgagtttggc ggaatggaat atatagatgc tggaacactc aaggaaccaa
     5041 actttgaggt gatttatgaa gcctctccag acctgataat agtatccggc agacaagtgg
     5101 acatgtatga tgattttgca agaatcgcac caactgtata tatgccaatg gacaatggtg
     5161 aataccttga gtcattaagg aataacctga atatattggg tcaaatattt gacaagaaag
     5221 agatggttga tgaaaaggtt gatgatattg agagctccat ccagtaccta aatgagattg
     5281 caagtgatag ggtcgagaac ggattgtttg tgatggctaa tgatgggaac cttagcgttt
     5341 atggggaagg ttcaagattt ggagtactac atgaggaatt tggaatccct gcagctgaca
     5401 gggagattga aagctcaaca catggtcaga agattacctt cgagtatatt cagcaaatcg
     5461 accctgagta catatttgtt atggaccggg cagcggttgc cgggggagaa gtatctgcca
     5521 agagtgtcat ggataatgaa ataattggta tgacaagagc ctatgctgaa ggcaagataa
     5581 tctatctgga tgcccacgtc tggtatgtag gtgcaggggg aattggaagt accgagaaga
     5641 tgataaatga aataatttca gccatagaat aggagtaaaa attccccata ttcaagagta
     5701 tggggaattt ttactccttt ttcaaccatt ggtaacaagt gttacggata tatttatgta
     5761 ttccacctat aatagacctt gtgagtaaca tcagagagaa tattttttag gaggaattga
     5821 taatatggct gagaatcaaa tgttttgcta tcagtgtcag gaagccgctg gtggaaaagg
     5881 atgtacaaag gtaggaatgt gtggaaagac aagtgaggtt gcaaatcttc aggacttgat
     5941 gatttatgga cttaagggac tttcagaact tggagttaag gctgatgaga tcggatacaa
     6001 aatggaaggt ctggacagac tgataatcga aggcctgttt atgacaatca caaatgcaaa
     6061 ctttgacaag gacagattct ttgagggcat caagcttgta cttaagaaga gggatgaact
     6121 gaaatcaata cttgaggaaa aaggcgtaga ccttcaagga ctaaaggatg cagcaacatt
     6181 tacagtaaac agcaacgatg aaattgtatt caagtcaaac agcgctgagg ttagtgttct
     6241 tgcaacggaa aatgaagatg taagatcact cagggaactt ataatctatg ggcttaaggg
     6301 aatggcagca tatgcagagc acgctgacaa tcttggattc gaggatgagg agatttcaaa
     6361 attcataaga aaggcgcttg tagcaaccat ggatgacagc atgagcgctg atgatcttgt
     6421 agcccttaca cttgagacag gtaagtatgg agttcagacc atggccctac tggacaaggc
     6481 aaacacttcc acttatggaa accctgagat aagcgaagtt aacataggag ttagaaacaa
     6541 cccagctatc cttgtatcag gacatgacct gaaggatttc gaagagcttc tggagcagac
     6601 aaaggatagc ggagtcgaca tatatacaca tggagagatg cttccagcaa actactatcc
     6661 aacattcaaa aaatacgacc actttgtagg aaactacggg aatgcttggt ggcagcagaa
     6721 ggaagagttt gacaagttca acggagctat cctctttacc acaaactgta tcgtacctcc
     6781 taaggcagac tatgctgaca gggtttacac aacaggatca tcaggatacc caggcttcaa
     6841 gcacattcca gacagagttg acggacagcc aaaggatttc tcagaagtga ttgagcatgc
     6901 caagagactc ccagctccaa ctgaaattga gactggcaag atcgttggag gatttgccca
     6961 cgcacaggta tttgcacttg cagacaaggt tgttgaggca gtgaagaccg gagctatcag
     7021 gaaattcttc gtaatggcag gctgtgacgg aagacaagga aagagaagct actacactga
     7081 attcgcagag gctcttccaa aggatacagt aatccttact gctggatgtg ccaagttcag
     7141 atacaacaag cttaacctgg gagacatcaa tggaattcca agagttttgg atgcaggaca
     7201 gtgtaatgac tcatattcac ttgctgtgat tgcaatgaag cttaaggaaa tcttcgagct
     7261 aaatgacatc aatgaccttc caatagccta caacattgca tggtatgagc aaaaggcagt
     7321 aattgtgcta ttggcactcc tacaccttgg agttaagaac attcaccttg gacctacatt
     7381 gccggcattc ctgtcaccaa atgtggcaaa ggtactggtt gatacattcg gaatcggaac
     7441 aatatcagat gtggaaaatg acatcaacat gttcctggga gaagcagcaa acgcataatc
     7501 tccccttatt cataggaaac ctcaccgaaa ttctttcggt ggggtttttt ggcctttaca
     7561 aatccttctt ttcatcatat agtactatta ttacacaaat atcagaaaag aaggtaagag
     7621 cttagatgat aatgagt
//
LOCUS       DASRQF010000162         4219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_141681, whole genome shotgun sequence.
ACCESSION   DASRQF010000162 DASRQF010000000
VERSION     DASRQF010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141681"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1577)
                     /locus_tag="VFD79_04445"
     CDS             complement(<1..1577)
                     /locus_tag="VFD79_04445"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HZH93330.1"
                     /translation="MFRFSFNRQLPLLLLAIVTLFLFPHQVEASGYDLEISELDISMD
                     IMEDNSYRIVEKITVQFNRSDMHGIFRDIPARTYFGKPVKIQDVDVVGHQFQSSREGD
                     YLSLRIGDPNEYSNPIEYYTINYVYNIGDDLNDEMDELYFNLVGDQWEIPIHRTLFTI
                     NMPKDFNPDDINFTAGYTGSTFRPQVEYTVNGTRISGEYRETISPGQALTIALPLDEG
                     YYSQVQPDGDGIRTFTKSYIALFPLLLFGAFGLLFKYGRDDPIYPTVEFYPPEDLTPA
                     EIGYIYDNRIDPYDLTSMLIYWADKGYLRIIEEEEEEGLIFKKKKTTLKLEKLKALPA
                     GTKRFESVYFNDLFNTYAIDNVVDIDQLKESFYITLGAVKSDLMNSFDNRRFLSRSGF
                     FASLGMSFIGMVAFVLVFLGIFNEMNPYSPGLVTAFAVIGGILLGGFLSGSATLFASV
                     KTKLPYERFKTIMAGIFTALVPLAIAILILFIAEARTIYLIGFAIGIVIIYLSPKAHK
                     RTPQGVKWMQHLLGLKN"
     gene            complement(1558..2106)
                     /locus_tag="VFD79_04450"
     CDS             complement(1558..2106)
                     /locus_tag="VFD79_04450"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HZH93331.1"
                     /translation="MGFLVAGGIILLIGVYVFSTYNGFIKIRNAVEEAFSTMDVYLKK
                     RYDLIPNLVETVKGYAKHEQETFQKIVEARAQAMNATTIEEKQASENVLTGTLKSLFA
                     LSENYPELKADSQFLDLQRQLQKVEEDIAQSRKYYNAVVKTMNTKVESFPSNIVAMLF
                     GFKRATYFTADETERENVQVQF"
     gene            complement(2214..3173)
                     /locus_tag="VFD79_04455"
     CDS             complement(2214..3173)
                     /locus_tag="VFD79_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93332.1"
                     /translation="MIKSYLYTVGKYLPASQRDDILRDLEANIYEQLEEAYGDREYSQ
                     REVEDVLFKMGNPRKVAENYLERPRYLIGPQYIDLYLLVIKVALIGISIGILVVSTMK
                     FIVGGDILESLLSLLGGIWNGGLVMVGMVTIIFALIEHYGQKDSLPFDQDDEKWTLDT
                     LESPPEDVDQLKISDLIFESIFILVFLAFINRGLPLPVIYEDQSFVLLNYEVLSPYLL
                     WINLALGLSLLLNVYLLIRRRWSILTRLISIALDMVGIGIFSVLALTPDLWDFSRIPG
                     VSQEQLIGIETGVGLGLKIGLAAIIVITLVGVFNHIRALLRKR"
     gene            complement(3175..3513)
                     /locus_tag="VFD79_04460"
     CDS             complement(3175..3513)
                     /locus_tag="VFD79_04460"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZH93333.1"
                     /translation="MDRHEELFNNLQQEMKRETLVLAVLAQLDKPQYGYSLMSILVEK
                     GLQIDQNTLYPLLRRLEKQGMLESIWDVEGSRPRRYYKISSLGLSVRRDLISEWKNLN
                     KVIGNLIGGN"
     gene            3777..4007
                     /locus_tag="VFD79_04465"
     CDS             3777..4007
                     /locus_tag="VFD79_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93334.1"
                     /translation="MDNLLEKAKDFERDIIEDRRHLHGNPEVGFNLPETAEYVKSRLR
                     EMGIEPKDCGGNNLGINQQAIVLAHAIGYGPF"
BASE COUNT         1271 a          878 c          842 g         1228 t
ORIGIN      
        1 aaatttttca aaccaagcag gtgctgcatc cacttaactc cttgtggtgt ccttttgtgg
       61 gcctttggtg acaaatagat aatcactata ccaattgcaa agccaatgag gtaaattgtc
      121 cttgcctcgg ctataaatag aatcagtatt gctattgcca gaggaacaag agcagtaaag
      181 attcctgcca taatggtttt gaacctttca tagggaagct tggtctttac acttgcaaag
      241 agggttgctg accctgaaag aaagcctcca agaagaattc cgccaattac agcaaaggct
      301 gttacaagtc ctgggctgta ggggttcatt tcgttgaaga ttccaagaaa taccagcaca
      361 aaggcaacca ttcctatgaa tgacattccc agggatgcaa aaaagcctga tctggacagg
      421 aacctcctat tgtcaaatga gttcataaga tcgcttttca cagctccgag ggttatataa
      481 aagctttcct tgagttggtc aatgtccacc acattgtcaa tggcatatgt gttaaaaagg
      541 tcattgaagt agacgctttc aaatctcttg gtacctgcgg gaagagcttt cagcttctcc
      601 aacttgagag tggtcttctt tttcttgaaa atcagaccct cctcttcctc ttcttcaata
      661 atcctcaaat aacccttgtc tgcccagtag atcagcatgg aggtcaggtc ataggggtct
      721 atacgattgt catagatata gcctatctca gctggggtca ggtcctcagg cgggtagaat
      781 tcaaccgtag gataaatagg gtcgtcccta ccatacttaa acagcagacc aaatgctcca
      841 aataacagta atgggaacaa ggcaatatag gattttgtga aggttcttat gccatctcca
      901 tcaggctgga cctggctata gtagccctca tcaaggggaa gggctatggt aagcgcctgt
      961 cctggagata tggtctccct gtattctcca cttatccttg taccgttaac agtgtactcc
     1021 acctgtggtc taaaagtgga gccggtatat cctgcagtga aattaatatc atctggattg
     1081 aaatcctttg gcatgttaat ggtaaataag gttctgtgga ttggaatttc ccactggtca
     1141 ccaaccagat taaaatacag ctcgtccatt tcatcattaa ggtcatcgcc tatattgtag
     1201 acataattga tggtatagta ctctatgggg tttgagtatt catttggatc acctatccta
     1261 agggataggt agtcgccttc tctggaggat tgaaattggt gtccaacaac atccacatcc
     1321 tgaattttaa ctggcttgcc aaagtaggtt cttgcgggta tatccctaaa aatcccatgc
     1381 atgtcagacc ggttgaactg gacagtgatt ttttcaacga ttctgtagga attatcctcc
     1441 atgatatcca tacttatatc aagctctgag atctccaggt cgtaaccaga ggcttccacc
     1501 tgatgtggga acaggaatag agtaactata gccaatagca gaagtggtaa ttgtctgtta
     1561 aaactgaacc tgaacatttt ctctctccgt ttcatctgca gtgaagtagg ttgccctctt
     1621 gaatccaaac agcatggcta ctatgttgga tgggaaggac tccaccttgg tgttcattgt
     1681 ctttacaaca gcattgtagt actttctgga ttgtgcaata tcctcctcga ccttttgcag
     1741 ctgcctttga agatcaagaa attgactatc cgccttcagc tcaggatagt tctcactaag
     1801 ggcaaacagt gattttagag tacctgtaag cacattctca gaggcctgct tttcttcgat
     1861 tgtggttgca ttcattgcct gtgccctggc ttcaacaatc ttctggaatg tctcctgctc
     1921 gtgcttcgca tatcccttta cagtttcaac aaggttaggg ataaggtcat aacgcttttt
     1981 aagatagaca tccattgtag agaatgcctc ctctacagca tttctaatct ttatgaatcc
     2041 attgtaggtg ctaaaaacat agacaccaat taatagtatt ataccgcctg ctacaagaaa
     2101 acccatgata tcactcctct tctttatttg tgtttattat accacttcca aacgggttct
     2161 tacatatgca tattaaaagg atacccagtt aaaggtatcc caaattataa atattatctt
     2221 tttcttaaaa gagcccttat atggttaaaa actccaacca gggtaatgac tattattgca
     2281 gccaaaccga ttttaagacc caatccaacg ccagtttcaa taccgatcag ttgctcttga
     2341 gaaactcctg gtattcttga aaaatcccat aggtctggag tcaatgcaag caccgagaat
     2401 attccaattc ctaccatgtc aagagcaatt gaaatgagcc tagtaaggat actccatctt
     2461 cttctgatca aaagatatac attcaaaaga aggctcaaac caagtgccag atttatccaa
     2521 aggagatagg gtgataatac ttcgtagttt agtagaacaa aggattggtc ttcatagatt
     2581 acaggcaggg gaagtccacg gtttatgaat gcaagaaata ctaagataaa aatcgattca
     2641 aaaatcaaat cagatatttt taactggtca acatcctcgg gaggtgactc cagtgtatcc
     2701 agggtccact tctcatcatc ctggtcaaat ggaagagaat ccttttgacc ataatgttca
     2761 ataagggcaa aaataatggt taccataccc accatgacca gaccgccatt ccagattccg
     2821 cctaacagcg acaaaagtga ttcaaggata tctccgccaa ctatgaactt cattgtggat
     2881 acaactaaaa ttccgatgga tattccaatg agtgcaacct tgatgacaag aaggtacaga
     2941 tcaatgtatt ggggaccaat gagatatctt ggtctctcaa ggtagttttc agcaaccttc
     3001 ctgggattac ccatcttgaa aagtacatcc tcgacctccc tttgggagta ttccctgtca
     3061 ccgtaagctt cttcaagctg ttcatagata tttgcttcaa ggtcccttag aatgtcatct
     3121 ctttgtgatg ccggaagata ttttcccact gtatagaggt agcttttaat catttcagtt
     3181 tcctcctatc aaatttccaa taaccttgtt caagtttttc cactctgaga tgagatcacg
     3241 tcttacagat aatccaaggg agctgatttt gtagtatctt ctgggtcggc ttccttcaac
     3301 atcccaaatt gactccagca taccctgttt ttccagtctt ctgagcaaag gatacagggt
     3361 gttctgatct atttgcaatc ctttttcaac caatatgctc atcagagagt atccgtactg
     3421 aggtttgtca agctgtgcca aaactgctag aacaagggtc tcacgcttca tttcctgctg
     3481 cagattgttg aataattctt catgcctatc cataaggcac cccccttcaa ttccattata
     3541 gtgtatgtcg cacagtattg tcaatataaa ccatagcaaa taatataaaa atatttttac
     3601 aaagtaaaaa aatatttata cacctgtccc agctgaaagg ggataatcat gactaaattt
     3661 ttagaaatat tgaagtcaag ggctttcaaa ctctgaaaaa gttttgaaag ccaacatcat
     3721 tttttggcac acaaattgca attattaata gcaatactaa tttaaggagg agaggaatgg
     3781 ataatttgct tgaaaaggcc aaggactttg agagagatat tattgaggac agaagacacc
     3841 tccacggtaa ccctgaggtt ggattcaacc ttccagaaac tgctgagtat gtaaaatcaa
     3901 gactgaggga gatgggaata gaaccaaagg attgtggtgg gaataacttg ggaatcaatc
     3961 agcaggcaat tgtgctcgca catgctatcg ggtacggacc cttctagatt aaatgaggct
     4021 attaagggta gaaattattg aaataaagat atggagatga aaaaatggat aaaaaaacct
     4081 ggataaggtg attgcattaa ggaaagagct tcaccagaat cccgaacctt caaatcagga
     4141 ggtttggacc aagaatagac ttatggaatt cgttaaggag aacacaaggc tagagattgt
     4201 agatggggga aagtttttc
//
LOCUS       DASRQF010000163         6487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1492874, whole genome shotgun sequence.
ACCESSION   DASRQF010000163 DASRQF010000000
VERSION     DASRQF010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6487
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492874"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VFD79_04470"
     CDS             complement(<1..222)
                     /locus_tag="VFD79_04470"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZH93335.1"
                     /translation="MTVTNDTIKTQLEHRTIREFTQEPVSKEIFDTLIHVAQRTATSN
                     GMQTYSIIRVTDPEKKKAISEVGRQEYISR"
     gene            complement(339..1181)
                     /locus_tag="VFD79_04475"
     CDS             complement(339..1181)
                     /locus_tag="VFD79_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93336.1"
                     /translation="MRIEFHSDTAKKNNEDIYGLTRRSAYVIDGASALTSRSFTPGGN
                     DVTWMVQWWKRFLDDNLDNTTRTIQEILKIGIMDFNRDFGRFADIDSLEAHEQLSAGI
                     GIVRKHGDVLEAYVLGDVEISLESKRGECLVVTDSSIKDLDSQVVDLMRRNHQREGQE
                     VFKGFTGQELDLLIRNRSRMNTPGGYFILGHSLDAVDMGIYKEFRVGDIEKCLLATDG
                     IVPLSLRYSRKSLLESIGKKGVREMIKELRGIEKSDSDRSRMGRLKVHDDATLVYLDF
                     GLQP"
     gene            complement(1355..1873)
                     /locus_tag="VFD79_04480"
     CDS             complement(1355..1873)
                     /locus_tag="VFD79_04480"
                     /inference="COORDINATES: protein motif:HMM:NF016705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HZH93337.1"
                     /translation="MGFYESPDYKVVETFGDIEIRKYSSYSIVTYDNPGDETSSQGFS
                     SLFSYISKGNKREEKISMTVPVIDEKKEDRTTMSFVVPSKFNKDDIPLPKSDFLSVRV
                     MEEGYFAAIRFRGNPTDKRLEENENRLRDFIRERGYRELSNYYNAFYDPPFQLPFIKR
                     NDIMVRIDYKEK"
     gene            complement(1887..2339)
                     /locus_tag="VFD79_04485"
     CDS             complement(1887..2339)
                     /locus_tag="VFD79_04485"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HZH93338.1"
                     /translation="MFKRKTPWLVVLIWMGLIFFLSSQEAADSNQLSTGITKFIMDLI
                     SGIFPGLSPQVQWLNHIVRKNAHFIAYLVLGILQVNALYLNGKRGGKAFILALLISFL
                     YAASDEFHQTFVPGRSGELRDVMIDTAGALTGIGTYMLIRMKWRKETT"
     gene            complement(2359..3822)
                     /gene="pepD"
                     /locus_tag="VFD79_04490"
     CDS             complement(2359..3822)
                     /gene="pepD"
                     /locus_tag="VFD79_04490"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HZH93339.1"
                     /translation="MINYDAIEGLEDRSFWKHFADISVIPRGSGVPQNQKGISNYVFS
                     FIEELGLEVYQDDHLNVVARKPASEGYEDEPPVMLQAHLDMICEKELYTSHDFTRDPI
                     RLIREGDIIMADGTTLGADDGIGVAFIMSILEDKTLKHPEIEAVFTTDEETDMGGAFN
                     LDYSKLNSKLILNLDAEAIGVCGSGELEVEMTLPIKREPVRKKSELCRIKIAGLKGGH
                     SGMNSMDERGNAILILTRLLLALQNKVDFRLADITGGQGMSSAFARNSTCVISYPSNA
                     REEVTGILEDEFEIIKRELERRDPAVKMDLVFQVEVYDQVIDGQTQDKLLDLLTILPD
                     GLFSLNREFPDAMESTTNIGVIETLEEEIFVTMLIRSFLPGKKYFLLRKTVRLCELLG
                     IEHRIGRDLPHWEYNVSEGLMKILNDVYPDTEFHASQATLEAGIFNMNIPGSTVISLG
                     SPYYEAHSPSEYLSIAETEDYWDMLISFLDRLKSEVD"
     gene            complement(3854..5005)
                     /locus_tag="VFD79_04495"
     CDS             complement(3854..5005)
                     /locus_tag="VFD79_04495"
                     /inference="COORDINATES: protein motif:HMM:NF022635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HZH93340.1"
                     /translation="MNNIMDYLDWRGDLSFERDPINEVDGLILSLISYNDFEGIVPGV
                     GSKDSVGLREAAERFKGEKDVSHLQDIPFLNEIPGLLHKAAETERYGALQLTWFVNQV
                     DPEATKQFAALTIKLTERLNFISFRGTDDTLAGWKEDLQMSFMEEVQSQKEAVAYTER
                     AMMSLRGDFILGGHSKGGNLAIYAASSMNELNKSRINEVYNNDGPGFQPEVVKREGYR
                     DIMDRVHTFLPESSIVGMLLEHGTEYEVVKSTGLAITQHNPFIWEVIGNEFILVDSLS
                     KESLNINTAIRSWITQLSYDERREFVDAMFQVLSETGATSLAELNNEKFGAADAMIKA
                     LKTMDSTAKVHLKDTIDLLFEEARKVIRKSIADDIDSFITRRKRKKFKE"
     gene            5360..6127
                     /locus_tag="VFD79_04500"
     CDS             5360..6127
                     /locus_tag="VFD79_04500"
                     /inference="COORDINATES: protein motif:HMM:NF019450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HZH93341.1"
                     /translation="MAAAMIAAKLMTPVSVAEADSFIKGDLGPETQVASIVDDNSDET
                     TDEISDDTFDDISDEISDEYRDDDSDETSDESLDEYSDEFSDENTDGISDYSTYDFSD
                     EYSDELSDEWDTDERRFELREDRSNLTLLRVGKEFYFVNDERARLDAPPLVEEGRTIL
                     PVRFIAQTMGSQVLWDQEDRRVTIIKGDDTIELWIDSNLARVNGDFKLIDPDNDSIRA
                     RIIPETGRTMVPVRFVAETLGADVFWDEATKTISLKY"
     gene            complement(6145..>6487)
                     /locus_tag="VFD79_04505"
     CDS             complement(6145..>6487)
                     /locus_tag="VFD79_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93342.1"
                     /translation="YEALKDGGYNPDFFVDGYVTGYLLIRKDDYRFIASEGQHRIAAL
                     AALGYDSFECRFDLKPWTNQTVFYNQAAKWPQVKAGIFTPRLAKMCFEHFFRKDNNHR
                     LNEHIKGMSSS"
BASE COUNT         1674 a         1602 c         1259 g         1952 t
ORIGIN      
        1 cctgctaata tactcctgcc ttccaacctc tgaaatggcc ttcttctttt caggatctgt
       61 caccctgatg attgaatagg tctgcatccc gttggaggtg gccgtcctct gtgccacatg
      121 tatcagtgta tcaaagatct ccttggagac tggctcctgt gtaaattctc tgattgtcct
      181 gtgttcaagc tgtgttttta tggtgtcatt ggtcactgtc atccttttac cccctttttt
      241 tctctaatcc acttataccc ttataggcta ttggtccaca caaaaaagag tggccaaggc
      301 cactcaagtt aagcttatcc aatttttcct gaaccaatct atggctgcaa accaaagtcc
      361 agataaacca aggttgcatc atcgtgtacc ttcaatcgtc ccattctact cctgtcagag
      421 tcggatttct cgattcctct caactccttg atcatttccc ttacaccttt tttcccaata
      481 ctctcaagta ggcttttcct ggaatatcta agactcaagg gaacaattcc atcagtggcc
      541 agaaggcatt tttcaatatc tcccacccta aactccttgt atattcccat gtccacagca
      601 tcaagactat ggcccagtat gaaatatcct ccaggtgtgt tcatcctgct tctatttctt
      661 atgagcaggt ccagctcctg tccggtaaag cccttgaaaa cctcctggcc ttccctttga
      721 tggttccttc tcatcaggtc caccacttgg gaatcaaggt ccttgattga gctgtctgtt
      781 acaaccaagc attctccacg cttgctttcc agtgaaattt caacatcacc caaaacatat
      841 gcttcaagga cgtccccatg ctttcttaca atcccaattc ctgccgacaa ttgttcatgg
      901 gcttccaatg aatcaatatc tgcaaatctc ccaaagtccc tgttgaaatc cataattcct
      961 attttaagta tttcctgtat ggtcctggtg gtattgtcca aattgtcatc caaaaatctt
     1021 ttccaccatt gcaccatcca agttacatca tttccaccgg gtgtaaagct tctgcttgtc
     1081 aatgcagaag ccccatcaat tacataggca cttcttcttg ttaggccata tatgtcctcg
     1141 ttgttctttt ttgccgtgtc tgaatgaaat tcaatcctca tgtcacacct catctatgta
     1201 gtgaactttc tttgacctat ttacaggccc taaataatga tagcaccttt aaaatatgga
     1261 tgtaacaaat attagatttt tctatgagga taatttttca ataagatttt cttatggcat
     1321 agaaaaacct gccttatcag caggtaattt cttcctattt ttccttgtag tcaattctga
     1381 ccatgatgtc attccttttg ataaagggca gctgaaaggg agggtcgtag aaggcattgt
     1441 agtagttgga caattccctg tagccccttt cccttatgaa gtccctaagt ctattttcat
     1501 tctcctccag ccttttatcc gtagggtttc ccctaaacct gatggcggca aaatagccct
     1561 cctccatgac tctgacactc agaaaatcac ttttaggcag ggggatatcg tccttgttga
     1621 acttggatgg aacaacaaag gacatggtgg tcctgtcctc cttcttctca tcgatgacag
     1681 gcaccgtcat ggagattttc tcctcccttt tatttccctt ggagatatag gagaacagac
     1741 ttgaaaaacc ctgactggaa gtttcgtctc ccggattgtc ataggtcact atgctatatg
     1801 aggagtactt ccttatctca atgtctccga aggtctccac aactttataa tccggacttt
     1861 catagaatcc catcctaata accctcttaa gtggtctctt ttctccactt catcctaatt
     1921 agcatgtagg taccgatacc tgtaagtgcc cctgccgtat ctatcataac atcccgcagt
     1981 tccccgcttc tacctggaac aaaggtctga tggaactcat ctgaggctgc atacaggaaa
     2041 ctgatcagga gggcaagtat aaaggcctta ccacccctct tgccgtttaa atacaaggca
     2101 tttacctgaa gaattccaag aacaaggtat gcaatgaagt gggcattctt tcttactatg
     2161 tggttaagcc actgaacctg aggtgatagc ccgggaaaaa ttcccgatat caggtccata
     2221 atgaattttg tgatgccggt actcagttgg ttggagtcag cagcctcctg gctgctgaga
     2281 aaaaatatca gacccatcca aatcaatacc accagccatg gtgtctttcg tttgaacatc
     2341 tatcttctcc ttaatacttt aatctacctc actcttcagc ctgtcaagga agcttattag
     2401 catgtcccag tagtcctctg tttcagcgat tgacaggtac tcgcttgggg agtgggcttc
     2461 ataatatggg gatcccaggg atatgactgt cgagcctgga atattcatgt tgaatatgcc
     2521 tgcctccagg gtagcctggc ttgcatggaa ctctgtatca ggatacacat catttagaat
     2581 tttcatcagc ccttcactta cattgtattc ccagtgtggc aggtctcttc caatcctgtg
     2641 ttcaatgccc aaaagctcac aaagcctgac cgtcttcctt aaaaggaagt acttcttgcc
     2701 tggaaggaag ctccgtatca gcattgtaac aaatatttcc tcctccaggg tctcaatcac
     2761 tccaatattg gtggtggatt ccattgcgtc aggaaattcc ctgttcagtg aaaaaagtcc
     2821 atccggtagt attgtcagca ggtccagcag tttgtcctgg gtctgaccat caatcacctg
     2881 gtcataaacc tcaacctgga agacaaggtc catctttaca gctggatctc tccgctccaa
     2941 ctctcttttg ataatttcga actcatcctc cagaatccct gttacctcct cccttgcatt
     3001 ggagggatat gaaataacac aggttgagtt tcttgcaaag gcagaggaca tgccctgacc
     3061 acctgttata tccgccaacc tgaagtcaac cttgttttgc aaggcaagta ggagccttgt
     3121 cagtattagg atggcattac ccctctcatc catggaattc attcccgaat gtccaccctt
     3181 aagacctgct atctttattc tgcaaagttc cgacttcttc ctaacaggct cccttttgat
     3241 gggaagggtc atttccactt caagctctcc tgaaccgcag actccgattg cctcagcgtc
     3301 caggttaagg ataagcttgc tgtttagctt tgaataatca agattaaatg ctcctcccat
     3361 gtctgtttct tcatcggttg taaaaaccgc ttcaatctca ggatgcttaa gcgtcttatc
     3421 ctccaggatt gacattataa aggcaactcc tatcccatca tcagctccca gggttgtacc
     3481 gtcagccatt atgatatcac cctcccggat gagccttatt ggatccctgg tgaagtcgtg
     3541 gctggtgtaa agttcctttt cacaaatcat gtccaaatgt gcctgaagca ttactggggg
     3601 ctcatcctca tagccctctg aagctggttt tcttgcaacc acattcaggt ggtcatcctg
     3661 gtagacctca agaccaagtt cctcaatgaa ggagaaaaca taattgctta tacccttctg
     3721 attttgtggt acccctgacc cccttggaat cactgaaata tcagcgaagt gcttccaaaa
     3781 ggacctgtcc tccaaaccct cgattgcatc gtagttaatc atctgttcat ctccttccat
     3841 tttaggttcc cttctactcc ttaaatttct ttctctttct tctggtgata aatgagtcta
     3901 tgtcatcggc tatggatttc cttattacct ttcttgcttc ctcaaatagg aggtcaattg
     3961 tatccttgag gtggaccttt gcagtggaat ccattgtctt cagggccttg atcattgcat
     4021 ctgcagctcc aaacttttca ttgttcagct ctgcaagaga tgttgctccc gtctctgaca
     4081 gcacctggaa cattgcatcc acaaattccc tcctctcatc ataggaaagc tgggtgatcc
     4141 aggacctgat ggcagtgttt atgttcaggc tttccttgct taggctgtca accagtatga
     4201 actcatttcc tatgacctcc caaataaagg gattgtgctg ggtaatggca agccctgtgc
     4261 tctttacaac ctcgtattcc gttccatgct caagtagcat tccaactatg gaggattctg
     4321 gcaggaaggt gtgcacccta tccataatgt ccctgtagcc ctccctcttg actacctcag
     4381 gttgaaagcc tggaccgtca ttattgtata cttcattgat tctggacttg ttgagttcat
     4441 tcatgctaga tgctgcatag atggccaaat tacctccctt tgaatgacct ccaagaataa
     4501 agtctcccct aagggacatc attgcccttt cagtgtatgc cactgcctcc ttctgggact
     4561 ggacctcctc cataaagctc atctgcaggt cttccttcca gccggcaagg gtatcgtcag
     4621 ttcccctaaa ggatatgaag ttcagtcttt cagttagttt tatggtcagt gctgcaaact
     4681 gctttgtggc ctcagggtca acctgattta caaaccaggt aagctgaagg gcaccatatc
     4741 tttcagtttc agcagcctta tgtaagagac ctggaatctc atttagaaaa gggatgtcct
     4801 gtaaatgtga cacatccttc tcccccttga acctttctgc tgcctcccta aggcctactg
     4861 agtccttgga cccaacccca ggaactattc cctcaaaatc attatagctt atcagggaca
     4921 gtattagacc gtcaacttca tttatggggt ccctttcaaa actcaggtct cccctccaat
     4981 ccagataatc cattatattg ttcatttgcc aactcacctt ccatcctaag ccgtattgtt
     5041 tcaaatatag tcaattgcac tattcattcc tacagcgtaa ctgccggccc atgttccttt
     5101 attttcctct ggccagccct tccctttcat tataccccgg aatcatactc ctagtgacat
     5161 aatccatttt ttgtttgttt ataccctgaa tgggtataat gaatacgtta gatgaatgca
     5221 gaattaatct tttgattctt gaaaggggta ttttaaaatg tccagaaagc tataaagtaa
     5281 tttaaaaaga cagcataggg tttgggagca gacacaggaa aaaagaagaa ggctattaag
     5341 gagatctgcg gcctcaacaa tggctgcagc aatgattgca gccaagctta tgactccggt
     5401 ttcagtggcg gaggcagact ctttcatcaa gggtgatttg ggacctgaaa cacaggtagc
     5461 atcaattgtt gatgataatt ctgatgagac aacagatgaa atctctgatg acacattcga
     5521 cgatataagc gatgaaatct ctgatgagta tagggatgac gactccgacg aaacatctga
     5581 tgaatcattg gatgaatact cagatgaatt tagtgatgaa aacactgatg ggatctcaga
     5641 ctattcaacc tacgatttct cagatgagta ttcagatgag ttatctgatg aatgggacac
     5701 tgatgaaaga agatttgaac ttagggaaga tagaagtaat ctcacccttc ttagggtagg
     5761 aaaggaattc tactttgtaa acgatgaaag agccaggctg gacgcacctc ctttggtaga
     5821 ggaaggaagg accatacttc cagtacggtt tatcgcccag acaatggggt cccaggtatt
     5881 gtgggatcag gaagaccgtc gggttacaat tataaagggc gatgacacca ttgaactttg
     5941 gatagacagc aatcttgcaa gggtcaacgg agatttcaaa ctgattgatc cagacaatga
     6001 cagcatccgt gcaaggatca ttcctgaaac cggcagaaca atggttccag tgaggtttgt
     6061 agctgagacc cttggggcgg atgtattctg ggatgaagct accaagacaa tttcattgaa
     6121 gtactagggt aaaaagggat gtcttcaact tgaagacatc cctttaatat gttcatttag
     6181 cctgtggtta ttgtcctttc taaaaaaatg ctcaaagcac atcttggcaa gccggggagt
     6241 gaaaatccca gccttaacct ggggccactt ggctgcctgg ttatagaaaa cagtctggtt
     6301 tgtccagggc ttaaggtcaa atctacattc aaaactgtca tagccaagtg cagccaaggc
     6361 tgcaatccta tgctggcctt cgcttgcgat aaacctgtag tcatcctttc gtatcaaaag
     6421 gtatcctgtt acatatccat ccacaaaaaa atctggatta tagccaccat cctttaaagc
     6481 ctcatat
//
LOCUS       DASRQF010000164         4650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1745856, whole genome shotgun sequence.
ACCESSION   DASRQF010000164 DASRQF010000000
VERSION     DASRQF010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1745856"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VFD79_04510"
     CDS             <1..246
                     /locus_tag="VFD79_04510"
                     /inference="COORDINATES: protein motif:HMM:NF014232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HZH93343.1"
                     /translation="KCYDAFGKELGNVIRGIHGHTKHNGKIPKRGYSMVRHQEDIDSL
                     DKELHEAIKQEEYERAAVLRDEIKRIRSIMDSDKRGE"
     gene            248..1264
                     /locus_tag="VFD79_04515"
     CDS             248..1264
                     /locus_tag="VFD79_04515"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HZH93344.1"
                     /translation="MTIWLDPQEYDQVVVSTRLRVARNIREEVFPLYSTRDQAMETAQ
                     EIIAAVEDQFPDGDYRLYRLNEMENRERVRFMENHIISPGLLDHIDKGSFFMRDDERV
                     TIMINEEDHIRLQTLLPGLDLVGGWELCSQIDDMLEDKLDFAYHTEYGYLTACPTNTG
                     TGLRASVMLHLPGVTMTGHIGSIINALRKIGLTIRGLYGEGTEAVGDLYQVSNQLTLG
                     ESEEEIIEKLHRVVQQLIKREKSTRAYFMEKKETELEDKVYRSLGILRSARILSSKEA
                     MKHISMVKLGSDIGILKDFRGSDLVRLMIWIKPGNVQVSEKRAMDKKERDKVRADLTR
                     EYFK"
     gene            1277..3715
                     /locus_tag="VFD79_04520"
     CDS             1277..3715
                     /locus_tag="VFD79_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HZH93345.1"
                     /translation="MGMFGRFTERAQKAILLAQEEAKKFNHNYVGTEHLLLGLIAEDQ
                     GIAATSLKELGVTIDNARKQVYDIVGMGEENVELVGFTPRTKKIFELAVTEARNLGHN
                     YVSTEHLILGLVGEGEGIAVTILRRMGVDPAKLADAVVQKLTQATPKQQQAQQQQQNG
                     KKGQSSLEKYSIDLNKMAQDGEIDPVIGRMKEIERVVQVLSRRTKNNPVLIGEPGVGK
                     TAIAEGLAQKIVEGDVPEIIKEKRIVTLDLPGMIAGAKYRGEFEERLKSVMKELKDAG
                     DVILFIDELHTIVGAGAAEGAIDASNILKPTLARGELQIIGATTIDEYRKHIEKDTAL
                     ERRLQPIMVEEPNIEDTIKILEGLRDRYEAHHGVKILDDALEAAAELSHRYIADRFLP
                     DKAIDLIDEAASKIRVNTYVAPDDLKDLEERLEELEHEKEEAINTQNFEKAANIRDKE
                     KDIKEQIESRKIKWQKHKQENNLVVGYDEIAEIVSNWTGVPVSRMTTEESERLLKLEE
                     VLHRKVVGQDQAVKAVSAAVRRARVGLKDPRKPIGSFIFVGPTGVGKTHLAKALAEAL
                     FGEEDAMIRIDMSEYMEKHSVSRLVGSPPGYVGYDEGGQLTESVRRKPYSVVLFDEIE
                     KAHPDVFNMLLQILDDGRLTDSKGRTVDFKNTVIIMTSNAGATSIKKQNVLGFSAAQD
                     KDIQEYDRMKEIITEELKRTFRPEFLNRLDEVIVFHSLKEDQVEEIVDIMVKDLEKRM
                     EKVGVNIKISAETKKYISKHGFDEVFGARPLERTIRKIIEDRLAEEILKGTVSKDENI
                     SVEYDGEKLTFNKA"
     gene            3748..>4650
                     /locus_tag="VFD79_04525"
     CDS             3748..>4650
                     /locus_tag="VFD79_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005955761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZH93346.1"
                     /translation="MAKKSKINRCSECGYEMVGFMGRCPECGAFGSFQEAERTETSSG
                     KRVRATSAAERLNSIQATNSQRVKTSINEFDRVMGGGIIKDSVTILTAKPGAGKSTLL
                     LQVANDLARQGINTLYASGEESTSQIKSRADRILKEIHDNLHVVSDNSLDSILEIIEK
                     TDARFIIVDSIQTFTLDGFPTSRAGSPTQTMECAYELVKVAKRPDKPRIVFIVGQMTK
                     DDELAGVRALEHLVDTVLVIEGESGEELRSLVSTKNRYGSTGEMGFFNMTEDGMVSID
                     NPSEYFMTKRSEENQVSGSALTVVR"
BASE COUNT         1477 a          902 c         1294 g          977 t
ORIGIN      
        1 aaatgctatg acgcttttgg taaggagctt ggcaatgtca tccgaggaat acacggacac
       61 acaaaacaca atgggaagat acccaagagg ggttactcca tggtcaggca ccaggaggac
      121 atcgatagtc ttgacaagga gctgcatgag gccataaaac aggaggaata cgagagagct
      181 gctgttctgc gggacgaaat caagaggatc aggagcatta tggattcaga taaaagaggt
      241 gaataggatg acaatctggc tagatccaca ggaatatgac caagttgtag taagcactag
      301 gctaagggtc gctagaaata ttagggaaga ggtttttccc ctgtattcaa ccagggacca
      361 ggccatggaa accgcacagg aaatcatagc tgcggtagag gaccaatttc ccgatgggga
      421 ctatagacta taccggttaa atgaaatgga aaaccgcgag agggttaggt ttatggaaaa
      481 ccacatcata agcccaggac ttcttgacca catagacaag ggcagctttt ttatgcgtga
      541 tgatgagagg gttaccatta tgatcaacga ggaggaccac atcagacttc agaccctttt
      601 gccaggtttg gacttggttg gcggttggga gctctgttca cagattgatg acatgttgga
      661 ggataagctt gactttgcct accacacaga gtacggctac ctaacagcct gtcctacaaa
      721 tacgggaacc ggacttagag catctgtgat gctccacctg cccggagtta caatgaccgg
      781 tcacattgga agcataatca acgccctgag aaaaattggg cttacaataa ggggtctcta
      841 tggagagggt acagaggcag taggagacct ttaccaggtt tcaaaccagc tgaccctggg
      901 cgaatccgag gaggagataa tcgaaaagct ccacagggtt gttcagcagc tgataaagag
      961 agaaaagagt acaagggcat actttatgga gaagaaggaa accgagcttg aggacaaggt
     1021 ctacaggtcc ctgggaatac tcaggagtgc aaggattctt tcctccaagg aggccatgaa
     1081 gcatatatcc atggtcaagc ttggctctga cattggaata ttgaaggatt tcagaggaag
     1141 cgacctggtc cggcttatga tatggatcaa gcctggtaat gtacaggttt cagagaaaag
     1201 agccatggac aagaaggaga gagacaaggt cagggcagac ctgaccaggg aatatttcaa
     1261 atgatggagg taaattatgg gaatgtttgg taggtttaca gaaagggcac aaaaagctat
     1321 tttacttgcc caggaagagg cgaagaagtt caatcacaat tacgttggaa ctgaacacct
     1381 tcttctgggg cttattgctg aagaccaggg tattgcagca accagtctga aagagctggg
     1441 agtcaccatt gacaatgcca gaaagcaggt ctatgacatt gtcggaatgg gtgaggagaa
     1501 tgttgaactg gttggattta caccaagaac caagaagata tttgagttgg cggtgactga
     1561 ggctagaaac ctaggtcaca attatgtaag cacggaacac ctgatccttg gtctggttgg
     1621 agagggagag ggaatagccg tgaccattct cagaaggatg ggggttgatc ctgccaagct
     1681 ggcggatgct gttgtccaga agcttacaca ggctacgcca aagcagcagc aggcccaaca
     1741 gcaacagcaa aatggcaaga aaggccaatc aagtcttgaa aaatacagca ttgaccttaa
     1801 caagatggca caggatgggg aaatcgaccc agtaatagga cgtatgaagg aaatcgaaag
     1861 ggttgtacag gtcctaagca gaagaaccaa gaataaccct gtgctcattg gtgaaccggg
     1921 agtgggaaaa acagccatag cggaggggct ggcccagaag atagtggaag gggatgttcc
     1981 tgaaatcatc aaggagaaga gaattgtcac cctggacctg ccgggaatga ttgccggagc
     2041 aaagtacagg ggggagtttg aagagaggct caagtctgtt atgaaggagc tgaaggatgc
     2101 aggagatgtg atccttttca tagatgagct ccatacaatc gtaggagcgg gggctgcaga
     2161 aggggcaatc gacgcatcca acatcctgaa gccaaccctg gcaaggggag aactccagat
     2221 aatcggagcc acaacaatag atgaatacag aaagcacatt gaaaaggata cggcccttga
     2281 aagaagactc caaccaataa tggtggagga gccaaatatc gaggatacaa taaagatact
     2341 ggaaggctta agggacagat acgaagccca tcacggcgtc aagatacttg acgatgccct
     2401 ggaggctgca gctgagcttt cccataggta tattgcagac aggttcctgc cggacaaggc
     2461 aatcgacctg atagatgagg ctgcttccaa aatcagggtg aacacctatg tagccccaga
     2521 tgacctcaag gaccttgagg aaaggcttga ggaactggag catgaaaagg aggaggccat
     2581 aaatacccag aacttcgaga aggcagccaa tataagggac aaggaaaagg acatcaagga
     2641 acagattgaa agcagaaaga taaagtggca gaaacacaag caagaaaaca accttgtggt
     2701 tggctacgat gagattgctg agatcgtgtc caattggacg ggagttcctg taagcaggat
     2761 gacaacagag gaaagtgaaa gactccttaa gcttgaggag gtccttcaca ggaaggtggt
     2821 tggacaggac caggcagtca aggctgtatc agccgcggtt agaagggcaa gggttggcct
     2881 taaggaccca agaaagccca ttggcagctt tatttttgtc gggcccactg gagttggtaa
     2941 aacccacctt gccaaggctc ttgccgaagc cctctttggt gaggaggatg caatgataag
     3001 gattgacatg agtgagtaca tggaaaaaca ctctgtctca agacttgttg gatcacctcc
     3061 tggatatgta ggctatgatg agggaggtca gctgacggag tctgtcagga gaaaaccata
     3121 ttcggttgtt ctattcgatg agattgaaaa ggcccacccg gacgtattca acatgctcct
     3181 tcagattctc gatgacggaa ggcttaccga ttccaaggga aggacagttg atttcaagaa
     3241 tacagttata attatgacat caaatgctgg tgccacctcc atcaagaaac agaacgtttt
     3301 gggcttttcc gctgcccagg acaaggacat ccaggaatat gacaggatga aggagataat
     3361 aacagaggag cttaagagga cctttaggcc agagtttctc aacaggctgg atgaagttat
     3421 cgtattccac agcctgaagg aggaccaggt tgaggagatt gttgatataa tggtcaagga
     3481 tcttgaaaag agaatggaga aggttggcgt taatatcaag atatcagctg aaaccaagaa
     3541 gtatatctcc aaacatggct ttgatgaggt gtttggtgca agacctctgg agaggacaat
     3601 cagaaagatc attgaagata ggcttgctga ggaaatcctc aaaggaacag tatcaaagga
     3661 tgagaatatt tcagttgaat acgacggaga gaagctcacc ttcaacaagg cttaaattaa
     3721 tgacaaagac cctgggggga gaaattattg gctaagaaaa gcaagataaa cagatgcagt
     3781 gaatgtggat atgaaatggt cggatttatg ggaaggtgcc ccgaatgcgg agcctttggc
     3841 tcctttcagg aggctgaaag gactgagact tcctccggca aaagggttag ggccacatcc
     3901 gctgcagaaa gactaaatag tatacaggct acaaacagcc agagggttaa aacctcaatc
     3961 aatgagtttg atagggtaat gggtggagga atcataaagg attccgtcac aatccttact
     4021 gccaagccag gtgctggaaa atcaaccctc ctcctccagg tggcaaacga cttggcaaga
     4081 caggggatta ataccctgta tgcatcaggt gaggagtcaa catctcagat aaagtcaagg
     4141 gctgacagga tactgaagga aatccacgat aatcttcacg tcgtatctga caatagcctt
     4201 gactcgatac ttgaaataat tgaaaagact gatgccaggt tcataatagt ggatagtatc
     4261 cagaccttca cactggatgg atttccaaca tctcgggccg gttcacccac ccagaccatg
     4321 gagtgtgcct atgagcttgt caaggttgca aaaagacctg acaagccaag gattgtattt
     4381 atagttggtc agatgacaaa ggatgatgag ctggcaggtg ttagggccct ggaacatttg
     4441 gtggatacgg ttctggtaat tgagggagag agcggcgagg agctgagaag tcttgtatcc
     4501 acaaaaaaca ggtatggatc taccggtgag atgggctttt tcaacatgac cgaggatgga
     4561 atggtatcaa tcgacaaccc ctcggagtac tttatgacaa aacgctctga ggaaaaccag
     4621 gtctcaggaa gcgccctgac tgtagtaagg
//
LOCUS       DASRQF010000165         8615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1550933, whole genome shotgun sequence.
ACCESSION   DASRQF010000165 DASRQF010000000
VERSION     DASRQF010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 8615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8615
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550933"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..592)
                     /locus_tag="VFD79_04530"
     CDS             complement(35..592)
                     /locus_tag="VFD79_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZH93347.1"
                     /translation="MKDILKRFDDHFIELLNSRIKNPYLDRFMLRITDLGGAVFISIL
                     ASGLVLLGDKNTKLLGLEAIVALFFGQIFVQSLKKLMSRERPYKIIEQLHTFGIDLRD
                     YSFPSGHTTASFSMATTIAVNMPRLTIFIFLLALLIGISRIYLGVHYPTDVAAGILLG
                     VFASLIIHLYFVDVVDRIAFFLGIN"
     gene            727..2595
                     /locus_tag="VFD79_04535"
     CDS             727..2595
                     /locus_tag="VFD79_04535"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HZH93348.1"
                     /translation="MKLSLTQIDAINHNQGPALILAVPGSGKTTVIIHRLNKLIKHHG
                     VKPERILSITFSKASAMDMSRRYSNTFPNEDSKPDFSTIHAFCFRIIRDYSKKKKITY
                     RLIEDDKDSLNKYRIIKELYQSLYSSAITEEKMEAFLSYYGYVKNMMIKPDELKGTKR
                     IDVSEFTQMYRMYEDVKHKNRFLDFDDLLTLSYGLLVDNPKALDYYRNKFDYIQLDEG
                     QDTSRIQLELLKLMAAPKDNIFIVADDDQSIYGFRGAYPQGLLDFRETYPQGRVFYME
                     ENYRSTRNIVNLSNNFIQQNKSRYVKNVSTNNPYSKAVQVVKFRTIEDQYSYILDNIG
                     KSPQSKVAVLYRNNLSALGLMNYLEKSNMQFYMRDIKLKFFNHWMIRDIYAFMELATD
                     PSKIEVFEQVFYKMKGYTSRKMINWLKGRNIERNVFKDLLKYPGISEFYRKQLLELEL
                     DFKKLQKMSPAKALRYIRLQLNYDSFLHENSLRFGYTFEQLKEMLYHMELIAAQTGNF
                     IHFKERMVTLKELMVKSSRNWCNLTLSTVHSAKGLEFDNVYIIDLVDRSFPSSASVEA
                     MEEGDHSLLEEERRLFYVGMTRAKHSLDLLFYQTLDGKNTDPSGFLSELEMIREKG"
     gene            complement(2625..3662)
                     /locus_tag="VFD79_04540"
     CDS             complement(2625..3662)
                     /locus_tag="VFD79_04540"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HZH93349.1"
                     /translation="MALLFAISMLMISCTPVAEELEEAGPETTDMASINDYYPFVHNS
                     TYLYEGYGNEYAQQNLLLEYENGQRGQYRIINPGTEAVRIIERTADSIREIYLEGEFY
                     HTENLLATTAAKNNILIKEPIQVGNKWELEGDNTREITGVDIEFQTPHKTYSALEITT
                     YLDEGRIQKDYYAQGVGLVGVVYEDGETTISSLLSEIVDGPYEKKARLFYPLESDLDI
                     VTTYVDHSFEFRTNDNLENTFEGFFKNPVEEGLNKVISEDTMINSIFLDKGTWTVRAD
                     FSKDLVPGWNAGSSLEIELIKSIVNTLGEFYDTDRVYLSIEGAPFESGHYGSTENEVF
                     QVDTQGIEEYK"
     gene            complement(3791..4516)
                     /locus_tag="VFD79_04545"
     CDS             complement(3791..4516)
                     /locus_tag="VFD79_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HZH93350.1"
                     /translation="MKRTVAFFDIDGTIFRNSLMIEHFKKLIAFELINPEVWYTKVKK
                     AYEDWEKRYADFEEYLEILAGVYLEELKGIKREHIEFVADNVIESNWDMVYKYSREQI
                     EWHRKMGHEVFFISGSPDFLVSKMAEKYGATEYRGTKYLVDEDNRYTGEIIRMWDSAS
                     KKRVLDEIIDRYQVDLVRSYAYGDTKGDLSMLRRIGNPVAINPNKELILMIKDDEYLS
                     KRISIIVERKNVIYKCDANIELL"
     gene            complement(4554..5609)
                     /locus_tag="VFD79_04550"
     CDS             complement(4554..5609)
                     /locus_tag="VFD79_04550"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZH93351.1"
                     /translation="MRVLRTILIMLATVILLFASFLAFITITDMKPDQMEILSVTNNQ
                     MKALPMGEAIVVTTYNIGYAGLDKTQDFFADGGQRSRAESLEAVEANMEAVIETLDGT
                     ESGILILQEVDRDSSRSFGMNQVDILESYYPNFGHVYGVNYKVPWVPVPITNPMGKVT
                     SGVMTLTRYNVSRAARYQLPGEENWPVSLFELDRCFTEIRMKTENGSEAVLLNLHLSA
                     FDKGGIIRLKQLEYLKDFLEEEYENGNYVIAGGDWNHNLPGTDPMNFEADEPWPFWLK
                     DIPEDYSVKGYNWAADPGNPTVRTLEMAYEPDYNFKAVIDGFLISDNIRIISVEGIDL
                     GFENTDHHPVRLSFSLE"
     gene            complement(5640..6503)
                     /locus_tag="VFD79_04555"
     CDS             complement(5640..6503)
                     /locus_tag="VFD79_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HZH93352.1"
                     /translation="MSIKIVCDSACDLPANLLEELGIEELPIVVIKGEKEYFDKVNID
                     PEEVYSSMRDGEVFKTAQITPITFQTSFEKHVKNGDQIIYIGFSSGLSATFQSANIAK
                     ISIQEKYPDADITLIDTKAASGGYGLVVYEAGTILKNGGSKEEIIEAVEFLSENVDHI
                     FTVDNIEYLYRGGRISRTNAILGGMLNIKPILEVNGGKLVPLEKIRGKNKVLKRMVDL
                     VEKRNIDSDLSNTIFCITHGDDLETAEKLKSMLIEKYGASEFITNHIGGAIGAHVGPG
                     ALTLCYSKRKR"
     gene            6638..7870
                     /gene="pepT"
                     /locus_tag="VFD79_04560"
     CDS             6638..7870
                     /gene="pepT"
                     /locus_tag="VFD79_04560"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HZH93353.1"
                     /translation="MENLRERFIRYVKTETTSDESSVSVPSTQTQLEFAKALAHELVE
                     IGMSEVTVDSKGYVMASLPSNTNRKLPVIGFIAHMDTSPDMSGKDVKPQIFENYKGGT
                     IQLNDELGIVLSPDDFPELNENIGKTIIATDGTTLLGADNKAGIAEIVTAMEYLINNP
                     EIRHGEIKVGFTPDEEIGRGADHFDVEKFGADFAYTVDGGPVGELEYENFNAATAKIT
                     IQGRNVHPGSAKNKMINSMIIGQELNGMLPVQQRPEYTEDYEGFFHLVRLDGTVEKTE
                     MVYIIRDHSMDKFQAKKKLLEKTADFLNSKYENIVHLEIKDSYFNMKEKIEPVIEIVH
                     LAKEAMENLGIDVGIKPVRGGTDGSRLSYMGLPCPNIFTGGYNFHGKYEFVTVEGMEL
                     ACNTILEIVKLSEKRPLE"
     gene            complement(7896..>8615)
                     /locus_tag="VFD79_04565"
     CDS             complement(7896..>8615)
                     /locus_tag="VFD79_04565"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HZH93354.1"
                     /translation="YEVIRVVKGNPMFLDDHLVRMVGSAKLADFKLPYDLDEIRDHVI
                     RLIASNGIVDNNIKLLATTDDTGKGLFMVYGVESFYPPESYYRQGIKVILFNHQRENP
                     NAKVQHSDFRQRVKIAMDEEKAFEALLVNEEGHILEGSRSNMFYVLEGRLFTAPASDV
                     LLGITRKHIIALSLRLGYAVHERRLHKDELGRVEGLFVSGTSIGVLPVSMVDDLKLPT
                     MGNEIISDLVEGYKSLMNDVS"
BASE COUNT         2523 a         1939 c         1590 g         2563 t
ORIGIN      
        1 tttgatcagc ttatattttt aattgcatcc cttgtcaatt tattccaagg aaaaaggcaa
       61 ttctatcaac cacatcaaca aaataaagat gtattatgag gctagcaaat actccaagca
      121 gtattccagc agctacatcc gttgggtaat gtacccctaa atagattctg cttataccga
      181 ttagaagtgc caataaaaat atgaatatgg ttagccttgg catattcacc gcaatggtgg
      241 ttgccatgga aaagctggca gttgtatgcc ctgaaggaaa cgagtagtcc ctcaggtcaa
      301 tgccaaaggt gtgcagttgc tcgattatct tgtagggtcg ctccctactc atcagctttt
      361 ttaaggactg tacaaatatc tgcccgaaaa acagggcaac tattgcttca agtcctagaa
      421 gcttagtgtt cttatcaccc agaagtacca gccccgaggc aagtatcgag atgaatactg
      481 cacctcccaa gtcggtgatc ctcagcataa atctatccag gtatgggttt tttatcctgg
      541 aatttaacaa ttctataaaa tggtcgtcaa acctcttcaa aatatccttc aaagtatcca
      601 cctcatttaa gctaccacaa ttatattaca ttgttacccg ataataaagg gtctaatatc
      661 agtttactac attattcctc atttttgtgt taaaatttaa gttgttaaaa cataggaggt
      721 atataaatga aattaagttt aactcagata gatgcaataa atcacaatca ggggccagca
      781 cttatccttg ctgtgccagg ttcaggcaag accactgtca taatacaccg attaaacaaa
      841 ttgataaaac atcatggggt caaaccggag aggatccttt ccataacatt tagcaaggcg
      901 tccgccatgg atatgagcag aaggtattcc aacacctttc caaacgaaga ctcaaaacct
      961 gatttttcaa caatacacgc cttttgcttt agaataattc gagattactc aaaaaagaag
     1021 aaaatcacat atcgccttat tgaagatgac aaggattcac taaacaaata tcggattatc
     1081 aaggaattat accaatccct ttactcatca gcaatcacag aggaaaagat ggaggcattc
     1141 ctgagctatt acggatatgt gaagaatatg atgattaaac ccgatgaact aaaagggaca
     1201 aagcgaatag atgtatccga gttcactcaa atgtatagaa tgtatgaaga tgtgaaacac
     1261 aagaacaggt ttctggattt tgacgacctt ctaaccttga gctatgggtt gctggttgat
     1321 aatcctaagg ctcttgacta ctacaggaac aagtttgact atatacagct ggatgaaggt
     1381 caggacactt caagaattca gcttgagctt ctaaaactta tggccgcgcc taaggacaat
     1441 atttttattg ttgcagatga cgaccagtca atatacggat ttagaggagc atatcctcag
     1501 gggttgctgg attttagaga gacttaccca cagggtagag ttttttatat ggaggaaaac
     1561 taccgttcca caagaaacat tgtcaacctt tccaacaact tcatccaaca aaacaaatca
     1621 aggtatgtaa agaatgtttc tacaaacaat ccctacagca aggctgtcca ggtggtgaaa
     1681 ttccgaacca ttgaagacca gtacagctac atccttgata atattggtaa gtccccacaa
     1741 agcaaggtcg ctgttcttta ccgaaacaac ctatctgccc ttggtctgat gaactatctt
     1801 gaaaagagca atatgcaatt ttacatgcga gatattaaac taaagttttt caaccattgg
     1861 atgatcaggg atatttatgc attcatggaa ttggcaacgg acccttcaaa gattgaggta
     1921 ttcgagcagg ttttctataa gatgaagggt tatacctcaa gaaagatgat taactggctt
     1981 aagggaagaa atattgaaag aaatgttttc aaagatcttc tcaagtaccc tggcatatct
     2041 gaattttaca gaaaacagct tcttgagctt gagttggact ttaaaaaact tcagaaaatg
     2101 tctcctgcca aggcccttag atacattcgg ctgcagctga actacgatag cttcctccat
     2161 gagaactcct tgcgattcgg gtatacattc gaacagctaa aggaaatgct ttatcacatg
     2221 gagttgatag ccgcacaaac tggaaatttc atccacttca aggagagaat ggtgacattg
     2281 aaggagctca tggtcaaatc ttcacgaaac tggtgcaatc taaccctgtc aaccgtccat
     2341 tccgccaaag gtcttgagtt tgacaatgtt tatatcatag atcttgtaga tcgttccttt
     2401 ccctcctcag caagtgttga agccatggag gaaggggatc attctctact ggaggaggag
     2461 agaagactgt tctacgttgg aatgacaagg gcaaaacatt cacttgactt gttgttctac
     2521 cagactctgg acggaaaaaa tacagaccct tcaggctttc tgtcagagct tgaaatgata
     2581 agagaaaaag gatagtttca actatgaaac tatccttttc aactctattt gtattcttct
     2641 atcccttggg tgtcaacctg aaaaacttca ttttctgtag aaccatagtg ccctgattca
     2701 aatggagccc cctcgattga aaggtatacc ctgtctgtat catagaactc accaagtgtg
     2761 ttcactatgc tttttatcag ttcaatctcc aatgatgaac ctgcattcca tccaggtaca
     2821 aggtcttttg aaaagtcagc cctgaccgtc caggttccct tgtcaagaaa gatgctgttg
     2881 atcatggtgt cttctgaaat caccttattc aaaccttcct caactggatt cttgaaaaaa
     2941 ccttcgaatg tattctccag attgtcgttt gtcctaaact caaaactatg atccacatag
     3001 gttgtcacaa tatccagatc actctccaag gggtaaaaca atctggcttt tttttcatac
     3061 ggcccatcca caatctcact taatagacta ctgatagttg tttcaccatc ctcataaaca
     3121 acccccacca agcctactcc ctgtgcataa taatccttct ggatccttcc ttcatccaga
     3181 tatgtggtaa tctcaagtgc actgtaggtc ttatggggtg tttggaattc aatgtcaacc
     3241 cctgtgattt ccctcgtatt gtcaccttca agctcccact tgtttcccac ttggattggt
     3301 tccttgatca gaatattatt cttggcagca gttgttgcta gtaagttctc cgtatggtaa
     3361 aattctcctt ccaaatatat ctcccttatt gagtctgcag ttcgttcaat gatccttaca
     3421 gcctcggttc ctggatttat aatcctgtat tgtcccctct gaccattctc atattccagg
     3481 agaaggttct gctgtgcgta ctcatttcca tacccttcat ataggtaggt ggagttgtgg
     3541 acaaatggat aatagtcgtt tatggaagcc atgtctgttg tctccggacc tgcttcctcc
     3601 agttcttcag ccacaggtgt acaggatatc atcaacatgg atattgcaaa taacaatgcc
     3661 atggttttta gtgttttcaa tcctctcact gtcaatcacc tctttcatat atccttcctc
     3721 accatgatta tacaccaggc agtttgagaa gaatttacga tttctttaca ataactataa
     3781 taattccacc ctagagaagc tcaatgtttg catcacattt gtatatgaca ttctttcttt
     3841 ccacaataat tgagattctt ttggagaggt attcatcatc ctttatcatt aggatcaact
     3901 ccttgttcgg gttgattgca acaggattgc caatcctcct aagcattgaa agatcacctt
     3961 ttgtatcacc ataggcatag ctcctgacca ggtcgacctg gtacctgtct atgatttcgt
     4021 ccaagaccct ttttttgctg gctgaatccc acattctaat tatctctcca gtgtagcggt
     4081 tatcttcatc aacaagatac ttggtccctc tgtattcagt ggccccatac ttttctgcca
     4141 tctttgaaac caggaaatct ggacttccgg atatgaagaa gacttcgtgc cccatttttc
     4201 tgtgccattc aatctgctcc cttgaatact tgtaaaccat atcccagttg gactctatta
     4261 cattgtcagc cacaaactct atatgctctc tctttatccc cttaagctct tcaagataaa
     4321 ctcctgccag gatttcaaga tattcctcaa aatccgcgta gcgtttttcc caatcctcat
     4381 atgctttttt tacctttgtg taccatacct ctggatttat taattcaaag gcgatcaatt
     4441 tcttgaaatg ctctatcatc agagaatttc tgaatatggt cccatctata tcaaaaaaag
     4501 ctaccgttct tttcatcata tccctctctt ccataccaat ttgccaagca agtctactcc
     4561 aatgaaaagc ttaatctaac aggatgatgg tctgtgtttt cgaaacccaa atcaattcct
     4621 tccacagaaa ttattctaat attgtcagat ataagaaatc catctattac agccttgaag
     4681 ttgtagtcag gctcataggc catttccagg gttctaacag ttggattgcc tggatccgca
     4741 gcccaattgt atcccttaac cgaataatcc tcaggaatat cctttaacca gaatggccac
     4801 ggttcatcag cctcgaaatt catgggatct gttcccggta aattatggtt ccaatctcca
     4861 cctgcaatta cataatttcc attttcgtac tcttcctcca gaaaatcctt caggtattcc
     4921 aattgcttga gcctgatgat tccaccctta tcaaaagccg acaggtggag atttaaaagg
     4981 acggcttcac ttccattctc agtcttcatc ctaatttccg taaagcaccg atccagttca
     5041 aataaactga ctggccagtt ctcctctcca ggaagttgat atctagcagc tctcgaaaca
     5101 ttgtacctgg taagtgtcat tacacctgat gtcaccttgc ccattggatt ggttattggt
     5161 actggaaccc atggcacttt gtagtttacc ccatagacat gaccaaaatt aggatagtag
     5221 ctttccagga tgtcaacctg gttcatccca aaacttctgg aggaatcccg gtctacctcc
     5281 tgtaagatca gaatgccgga ttctgtacca tccagggttt caattacagc ttccatgttt
     5341 gcctcaaccg cttccagact ctcagccctt gacctttgtc ccccgtctgc aaaaaagtcc
     5401 tgggtcttgt caagacctgc gtagcctata ttgtatgttg tcacaacaat ggcttcaccc
     5461 attggtaggg ccttcatttg attgttggtt acagacagta tttccatttg atccggcttc
     5521 atatccgtaa ttgttatgaa ggctaaaaat gacgcgaaca gtaggattac tgtggccagc
     5581 atgataagta ttgttcttag aactctcatg aaatcctcct tctacgtcat tttattgtgt
     5641 tatctttttc tctttgaata acaaagtgtc aaagcgcctg gacctacatg cgctcctatg
     5701 gctccaccga tgtggtttgt tatgaactca gatgctccat acttttcaat tagcatggat
     5761 ttcaattttt ctgctgtctc caaatcatct ccatgagtta tgcagaatat tgtatttgaa
     5821 aggtcagaat caatatttct cttttcaaca aggtcaacca ttctcttgag taccttgttc
     5881 ttacccctta tcttctccaa aggaaccagt ttcccaccat tgacttcaag tatcggcttt
     5941 atattaagca ttccacccaa tattgcattt gtccttgata tccgtccacc cctgtacaaa
     6001 tactctatgt tgtccactgt aaagatatgg tccacatttt cactgaggaa ctctacagcc
     6061 tcaatgatct cttccttact acctccattc ttaagtatgg ttcctgcctc atagaccacc
     6121 aggccataac ccccagaagc agccttggta tcaatcaagg taatgtctgc atcagggtat
     6181 ttctcctgaa tcgaaatctt cgcaatattg gcagactgaa aggttgcgga caatccagat
     6241 gaaaatccga tgtatatgat ttgatctccg ttcttaacat gcttctcaaa tgaagtctgg
     6301 aaagtaattg gtgtaatctg agcggtttta aatacctccc cgtctctcat gctgctgtag
     6361 acttcttcag gatctatgtt aaccttgtca aaatactcct tctctccctt gattacaact
     6421 atgggaagct cctcgatccc cagttcctcc agcagatttg ccggcaagtc acatgcacta
     6481 tcacaaacta tcttgatgct cattagaacc cctccattaa taaatacatt tttgtttatt
     6541 tacccaatat tcttctattt tagcacattt gtataatttt tgatattatt gaatacattc
     6601 atatggaaaa tggtacaata gtagcggagg gatgaaaatg gaaaatttaa gagaaagatt
     6661 cataagatat gtcaaaactg aaaccacatc agatgaaagc tcggtcagtg tcccaagtac
     6721 ccagacacag ttggaatttg ccaaggcttt ggcccatgaa cttgttgaga ttggcatgtc
     6781 tgaggttaca gttgacagca aaggctatgt aatggcaagc ttaccgtcca atacgaacag
     6841 gaaacttcca gtgattggtt tcattgccca catggataca agtccggata tgtccggtaa
     6901 ggatgtcaaa ccccagattt ttgagaatta caagggaggt acaatacaat taaacgatga
     6961 gctaggaatt gtattatcgc cagatgattt cccagagctg aatgaaaaca ttggaaagac
     7021 aattattgca actgatggta caaccctgct tggtgcagac aacaaggcag gcattgcaga
     7081 gattgtaaca gccatggaat acctgatcaa taatcctgag atacgccacg gggagattaa
     7141 ggtaggcttt actcctgacg aggagattgg taggggagca gaccatttcg atgttgaaaa
     7201 gttcggagcc gactttgcct acacagtgga tggcggaccg gttggagagc ttgagtatga
     7261 gaatttcaat gcagcaactg caaaaataac aattcaaggc agaaacgtac atcctggctc
     7321 agcaaagaac aagatgatca attcaatgat aataggtcag gaactaaatg ggatgctacc
     7381 agtccagcag cgtcctgaat atacagaaga ctatgaagga ttcttccatc tggtgaggct
     7441 tgatggtaca gtggaaaaaa cagaaatggt ctatataatc cgagaccatt ccatggataa
     7501 gttccaggct aagaagaagc ttttggaaaa aaccgcagac ttcttgaact caaagtatga
     7561 aaacatagtc caccttgaga taaaggattc ctacttcaac atgaaggaaa aaatcgagcc
     7621 tgtcattgaa atagtccacc ttgccaaaga ggccatggaa aaccttggaa tagatgttgg
     7681 cataaagcct gtaaggggtg gcacagacgg ttcacgattg tcatacatgg gcttgccctg
     7741 tcccaacata ttcaccggtg gctacaattt ccatggaaaa tatgaatttg ttacagttga
     7801 aggcatggag cttgcctgca ataccatatt ggaaatagtc aagctttctg aaaaaagacc
     7861 tttggaataa ccaaaggtct tattcactag cttaactagg atacatcgtt cataaggctt
     7921 ttatatcctt ccaccaggtc actgattatt tcatttccca tagtcggcag tttcaggtca
     7981 tcgaccattg aaacaggcag aacacctatg ctggtacccg atacaaaaag accctcaacc
     8041 ctacccagct catccttgtg aagtcttctt tcatgtacag cgtaaccgag tctcaaggat
     8101 agggctatga tgtgctttct ggttatacca agaagaacat cacttgcagg agctgtgaaa
     8161 agccttccct caagaacata gaacatattc gacctgctgc cttcgagaat gtgtccttcc
     8221 tcatttacaa gaagagcttc aaatgctttt tcctcgtcca tagcaatttt aaccctttgt
     8281 ctgaaatcac tgtgctgcac ctttgcattt ggattctccc gctgatgatt aaaaagtata
     8341 accttgattc cctgtcggta atagctttca ggtggataga agctttcgac cccataaacc
     8401 atgaacaagc ctttgccagt gtcatcagtt gtggcaagca gcttaatgtt gttgtccact
     8461 atcccgttgc ttgcaatcaa cctgatcaca tggtccctga tttcatctaa atcgtaagga
     8521 agtttgaagt cagcaagttt ggcagaccca accattctta caagatggtc atcaagaaac
     8581 attggattcc ctttgacaac cctaatgacc tcata
//
LOCUS       DASRQF010000166         2904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_875499, whole genome shotgun sequence.
ACCESSION   DASRQF010000166 DASRQF010000000
VERSION     DASRQF010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875499"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VFD79_04570"
     CDS             <1..241
                     /locus_tag="VFD79_04570"
                     /inference="COORDINATES: protein motif:HMM:NF027650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter C-terminal domain-containing
                     protein"
                     /protein_id="HZH93355.1"
                     /translation="LKFSFNEAREWETIEDDLEELQNRLEQIDVEMQQAASQYSKLQD
                     LIDRKEKLLDEQEKMMERWLYLSEKAEMIEKEKES"
     gene            274..2433
                     /locus_tag="VFD79_04575"
     CDS             274..2433
                     /locus_tag="VFD79_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004822267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HZH93356.1"
                     /translation="MKKVVLAEKPSVARDIARVLGANGKRNGYMEGDKYIVTWALGHL
                     VTLAEPEDYNKSYKTWNMEDLPMLPSPMKLQVIKGSSKQFHLVKEAINRKDVGEIVIA
                     TDAGREGELVARWILEKIGVRKPIKRLWISSVTDKAIKDGFARLKNGKDYENLYASAQ
                     ARAEADWYVGINATRALTVKYNTPLSCGRVQTPTLQMIASREEEIRSFVPKDYYGIKV
                     EVDKVTLTWRSPENKDIKTFDMAARDKAMESMKKGKVKVTSLQKKTKKQYAPALYDLT
                     ELQRDANSRFGFSAKETLSIMQGLYENHKVLTYPRTDSRYLTGDIVETIPDRIKAIST
                     GSYRDLARPLLKTTIKADKSFVDDKKVSDHHAIIPTEQPLELSLLNDREKKILDLVVK
                     RFLSVLYPPYQYEEITLVGELEGNELYAKGRKVLDLGFRQVFATEDDEETQDLPDCKT
                     GDILPVKRVYGTEGKTGPPGRFTEGTLLSAMENPVKYMSTNNQELKGIISDTGGIGTV
                     ATRADIIEKLFNSYLIEHRGKEILLTGKGKQLLQLVPDDMKSPDLTARWERRLSEIAE
                     GKLKKDKFIGDIIQYAKKVTKEIKDSQETFRHDNLTRTKCPECGKFMLEVKTKRGKMY
                     LCQDRECGHKKTISTLTNARCPQCKKKLELKGEGDDRIFACSCGYREKLSQFEERKKK
                     EKGRLGKREVAQYMKKQEKKDDDINNGLADALSKLKL"
     gene            2579..>2904
                     /locus_tag="VFD79_04580"
     CDS             2579..>2904
                     /locus_tag="VFD79_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HZH93357.1"
                     /translation="MSTTSNYKVEEITKDNPLIDSLRTTIETNFYGNNVTKINSISEA
                     YKLAKESPGTIVTDMSVFRPEELGLSTDSKVLLFNDGAVTGRFAGARVIMGEANVNKT
                     EYTTIA"
BASE COUNT         1008 a          572 c          746 g          578 t
ORIGIN      
        1 cttgaagttc tccttcaatg aagccaggga atgggaaaca attgaggatg acctggaaga
       61 gcttcagaac aggcttgaac agatcgacgt tgaaatgcaa caggcggcat cccagtactc
      121 caaactccag gaccttattg acagaaagga aaagcttctt gatgaacagg aaaaaatgat
      181 ggaaaggtgg ctctatctgt ctgaaaaagc tgagatgata gaaaaggaaa aggaaagcta
      241 ggcagtgtac ctaggatgtg aaaggaagag aagatgaaga aagtggtatt ggcagaaaaa
      301 ccatctgtgg ccagagacat agcaagggtt ctcggggcaa atggaaagag aaatggatac
      361 atggaaggag acaaatatat cgtcacctgg gccttgggac acctggtgac ccttgcagag
      421 cctgaagact acaacaagag ttacaagacc tggaacatgg aggatttacc catgcttcca
      481 agcccaatga agctccaggt tattaagggt agcagcaagc aatttcactt agtcaaggaa
      541 gccattaaca ggaaggatgt gggtgaaatt gtaattgcta cggatgcagg acgtgaggga
      601 gagttagttg cccggtggat cctggaaaag ataggtgtca gaaagcccat aaaaagactt
      661 tggatatcgt ccgttacaga caaggcaata aaggatgggt ttgcaaggct taagaacggc
      721 aaggactatg aaaacctcta cgcatcagcc caggctcggg cagaggctga ctggtatgtg
      781 ggaatcaatg ccacaagggc tttgaccgta aaatacaaca ctcccttgtc ctgtgggagg
      841 gttcagaccc caaccctgca gatgatcgca agtagggagg aagaaatcag gagctttgtt
      901 cccaaggact attacggaat aaaggtggaa gtggacaagg tgactcttac ctggagaagt
      961 ccggaaaaca aggacataaa gacctttgat atggctgcaa gggacaaggc catggaatcc
     1021 atgaaaaagg ggaaagtcaa ggtcacttcc cttcagaaga aaaccaagaa gcagtatgct
     1081 ccagccctct atgaccttac ggagctgcaa agggatgcaa acagcaggtt tgggttttca
     1141 gcaaaggaaa cactttcaat aatgcaggga ctatacgaaa accataaggt gctcacatat
     1201 ccaaggacag actccaggta cctcactggg gatattgttg agacgattcc cgacagaatc
     1261 aaggccataa gcacaggaag ctacagggac cttgcaagac cccttcttaa gaccaccatc
     1321 aaggctgata agtcatttgt ggatgataag aaggtttccg accaccatgc aatcatacct
     1381 acggaacagc cccttgagct ttcacttctt aacgacaggg agaagaagat acttgacctg
     1441 gtggtgaaga gatttctaag cgtcctctac ccaccctacc agtacgagga aataactctg
     1501 gttggagaat tggaaggaaa tgagctttat gccaagggta gaaaagttct ggacttgggc
     1561 ttcagacagg tttttgccac agaggatgat gaggagaccc aggaccttcc agactgtaag
     1621 acaggtgaca tcttgccagt gaaaagggtt tatggaacgg aaggcaagac cggtccacca
     1681 ggaagattta cggaaggaac cctgctttcc gccatggaga accctgtaaa atacatgtca
     1741 accaataacc aggagctaaa ggggataata agcgacaccg gggggattgg aacagtagcc
     1801 acaagggctg atataatcga gaagctgttc aacagctacc taattgagca cagggggaag
     1861 gagattcttc tgacaggaaa gggtaagcag cttttgcaac ttgtaccaga tgacatgaaa
     1921 tccccggacc tgactgcaag atgggaaaga aggctttcag aaatcgcgga aggcaagctg
     1981 aagaaggaca agttcattgg ggacattatc caatatgcaa aaaaagtcac caaggaaatc
     2041 aaggatagcc aggagacctt tagacacgac aacctgacca ggaccaaatg ccccgaatgc
     2101 gggaagttta tgctggaggt gaaaaccaag aggggcaaga tgtacctttg tcaggacagg
     2161 gagtgtggtc ataaaaagac aatttcaacc cttacaaatg caagatgccc acaatgcaag
     2221 aaaaagcttg aactaaaggg ggagggagat gacagaatat ttgcctgctc ctgcggctac
     2281 agagaaaagc tctcccagtt tgaggaaaga aagaagaagg aaaagggcag acttggcaaa
     2341 agggaagtag cccaatacat gaaaaagcag gaaaagaagg atgatgacat caacaacggt
     2401 cttgctgatg ccctttcaaa gctaaagctg tagaaagaac acaaataatc cgcttgaatt
     2461 acaaaatcag gcggattttc cttgagaaga aattcctaat atgttataac aagagtgtaa
     2521 tgataaatac atataacaaa aactaagggg ttaagagaga atattacgga gggaagacat
     2581 gtcaactaca tcaaattaca aagttgagga aataacaaag gataatccac ttatcgattc
     2641 gcttagaaca acaattgaaa ccaattttta cggaaacaat gtcaccaaga taaactcaat
     2701 cagtgaagcc tacaagcttg ccaaggagtc tccaggtacc attgtaacgg atatgtccgt
     2761 attcagacca gaggaactgg gcctttcaac tgactcaaag gtcctcctct tcaatgatgg
     2821 agctgtaaca ggaagatttg ccggcgccag agtcataatg ggagaagcga atgtaaataa
     2881 aacagagtac accacaatag ctag
//
LOCUS       DASRQF010000167         5709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1445203, whole genome shotgun sequence.
ACCESSION   DASRQF010000167 DASRQF010000000
VERSION     DASRQF010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5709
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445203"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VFD79_04585"
     CDS             <1..402
                     /locus_tag="VFD79_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZH93358.1"
                     /translation="KPNMRASIVDLKLGVEVDNLQVSRIYPKAFITMSELGVKENRQT
                     VEIGLPENLNDLSYGLELKTRVIVDQAEEALLIPKDAVYLRSGSKYVQVLEDGEPVER
                     EIETGIEDNNLIQVTGGLTEGEEVVLNYLED"
     gene            433..1287
                     /locus_tag="VFD79_04590"
     CDS             433..1287
                     /locus_tag="VFD79_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZH93359.1"
                     /translation="MIKVRNLFHSYSNDENYAVNDISFQVDKGEIFGFLGPSGAGKST
                     TQNILVGLLQLQKGEVQVAGYDVKEIKNKMFNKIGMSFEQSNVYNKMSGLENLEFYGK
                     LFDVKTRDPMDLIRMVGLDGKEKVKAGEYSKGMKHRLTFARSMINYPEIWFLDEPTTG
                     LDPAIASNIKDIIKEENDKGVTVFLTTHNMFIADELCDRVAFIVDGKIRLIDSPKQLK
                     LKYGEKLVEVEYYRDGDLLKESYSTVVEEEKDKLIEVIRNNDIQTMHTKEATLEEIFI
                     KVTGRGLV"
     gene            1288..2403
                     /locus_tag="VFD79_04595"
     CDS             1288..2403
                     /locus_tag="VFD79_04595"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZH93360.1"
                     /translation="MKLWYSFIKELKLSSKSWYFYIELGMALVLLLILLFVVPENFDS
                     KGEEYIYLDLPQVVRDRFREDLLEEDLDQTSQMVQVEANKNVYVAEFFETEESKIHLL
                     DSMETLTAFSESERVPAVHVRVNEKNQIVQTYYLQGYETQKLRNLLLVFQNRKAGLDV
                     IEDYSDNIIVKSMYQDAEPLNDRENIIPVFLTFNGSLMSLFIIAAYIFLDKDEGIIKA
                     YAVTASSVWHYLLSKIGVIIITSIATTLMVTVPIMGTQPNYPVMILFLVTSGFFAASL
                     GLFLTSYYKDIAQAFGALYIVIVILIMPNISYFTPSWDPDWVKIIPSYVMLQSFKEII
                     SVNGNMSYVILASLGFAVLGMDIFLLANYRFKKTLTL"
     gene            2419..3423
                     /locus_tag="VFD79_04600"
     CDS             2419..3423
                     /locus_tag="VFD79_04600"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZH93361.1"
                     /translation="MIRKILLLFQRDLKVSVRNFITLYIIIVPVMFALMINVFSPGIN
                     DTTVEIVLLEGENPQQEEYFSQFAKVETLKTVTDVEERVLKRDNIVAVLPDGGDGYFI
                     LRQGNEPDYIIDYVRNLTAFDHYQIGIEDSTAEIVDYGRTIPPLKKIMVNVALIFTSI
                     MGGMIIAINIVEEKVDNTISAIHLSPVSRLGFIAGKSLIGVFVPIVGSFILMFFTGFR
                     DINYFHAFIMVVTTCLISLTVGFVEGVNNDDIMTAAGNMKLLFMPLFGSIAGVELLAD
                     KWQPLFYWIPFYWAYKGNDLVLTSSGTWLDVLKYSGIVFGISVLVFILLAPRIRKGLE
                     "
     gene            3474..3671
                     /locus_tag="VFD79_04605"
     CDS             3474..3671
                     /locus_tag="VFD79_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93362.1"
                     /translation="MSLWGVLAVVYSAAVVVITVVKPEKIWNMKKIELFKKVLGEKGT
                     EIFFYIWALIFLVLGIWLITR"
     gene            complement(3707..4714)
                     /locus_tag="VFD79_04610"
     CDS             complement(3707..4714)
                     /locus_tag="VFD79_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93363.1"
                     /translation="MVSLVRSGPRSLLISTSDKDELIRFLCDELDGVLVDMETVMKRS
                     EENHTIVYIIEYENKAKTEYRKTDALVVFMDPLVLACRLRSMVAHGLIETMRSGPQVL
                     ILRSAGNLGAVLDKVMEDFGGQLGSLDECGENGFEDRTIIGITEKPIYISMAADDFHP
                     QFIRLDQDYFNVKQVLRRHSIKYLNLGVGSKGWSNLEIRIYDSFGAYELHYKRLLTVL
                     DALQIGLLTGESWSKDQPRALLFIDVYSIKLLTFYSSQEVKKILFGLEYLDDGRRIID
                     YDLFENGKKVQWASLFKKEKNVDKKEKASEKRRELFSKLGESTINELLEIEKEILKTR
                     Y"
     gene            complement(4739..5356)
                     /locus_tag="VFD79_04615"
     CDS             complement(4739..5356)
                     /locus_tag="VFD79_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HZH93364.1"
                     /translation="MNKVKRFINLLLGRINVDQELMDTHKTVLLHISDTPSIIYPEIK
                     RVIRLIRPDYIVHTGDIADNIKLGLHQSYMARYKHEAKKILGILDTAQAKGIYLSLGN
                     HDNLDFISNNSNRINVVEDKMLLSIGDKTFAISHYSESLMDLDADVYLYGHDLSIKSQ
                     TIDSKIYLNGISAIHIIDLETLEVIKLDYPADTDNERLKRRKIKL"
BASE COUNT         1667 a         1117 c         1317 g         1608 t
ORIGIN      
        1 aagcccaata tgagggcatc catagtggac ctgaaacttg gagttgaagt tgacaatctt
       61 caggtaagca ggatataccc caaggccttt ataaccatgt cagagcttgg agttaaggaa
      121 aatcgccaga ctgtggagat aggacttcca gagaatttaa atgacctgtc ctacggactg
      181 gaactcaaga ccagggtgat tgtggaccag gcggaggaag cacttttgat tcctaaggat
      241 gcagtctacc ttagatctgg aagcaagtat gtccaggtac tggaggatgg ggaacctgtg
      301 gagcgggaaa tcgagacagg cattgaggac aataacctaa tccaggtaac aggaggcttg
      361 actgaagggg aagaggtagt tctaaactac ttggaggact agaactttga tggtcatgaa
      421 agaagggata ggatgattaa ggtaaggaat cttttccact catacagcaa tgatgagaat
      481 tatgcagtaa atgatattag cttccaggtg gataaaggag aaatatttgg ctttcttggc
      541 ccctccggag caggaaaatc cacaacccag aacatacttg tgggcctatt gcagctgcaa
      601 aagggagagg tccaggtggc tgggtacgac gtcaaggaaa taaagaacaa gatgttcaac
      661 aagataggca tgtccttcga gcaatccaat gtgtacaaca agatgtcagg ccttgagaac
      721 cttgagttct acggcaagct ttttgatgtg aagaccaggg atcccatgga cctgatccgg
      781 atggtgggcc tggatggcaa ggaaaaggtc aaggctggag agtattcaaa gggcatgaag
      841 catagactga cctttgcaag gtccatgata aactatccgg agatatggtt cctggatgag
      901 ccgaccactg gtctggaccc ggccatagca tcaaacataa aggatataat caaggaggaa
      961 aatgataagg gtgtcacggt ttttctgacc acccacaaca tgttcatagc cgatgagctg
     1021 tgtgacaggg ttgccttcat tgttgacggt aaaatcaggc tgatagattc tccaaagcag
     1081 ctgaagctca agtacggtga gaagctggtg gaggtggaat actacaggga tggtgacctt
     1141 ctcaaggaaa gctactcgac ggtggttgaa gaggaaaagg acaagctgat agaggtaatc
     1201 agaaacaacg atatacagac catgcataca aaggaagcca ctcttgagga gatattcatc
     1261 aaggtaacag gaaggggctt ggtctaaatg aagctgtggt acagttttat aaaggagtta
     1321 aagctctcct caaaaagctg gtatttctac attgagctgg gtatggcact tgttctgctc
     1381 cttatactcc tatttgtggt cccggaaaac tttgacagca agggagagga gtacatttac
     1441 cttgacctgc cacaggtggt aagggatagg ttcagggagg acctcctgga ggaggatttg
     1501 gaccagacat ctcagatggt ccaggttgag gcaaataaga atgtgtatgt tgctgagttc
     1561 ttcgagacag aggaatcaaa gatccatctc ctggacagca tggagaccct gactgcattt
     1621 tcagagtctg aaagggttcc tgcagttcac gtccgtgtca atgaaaaaaa tcagattgtc
     1681 caaacctact atctacaagg atatgagacc cagaaactaa gaaacctcct gctggtcttt
     1741 caaaacagaa aagcaggact ggatgtaatt gaggactact cagacaatat aattgtaaaa
     1801 tcaatgtatc aggatgctga acccctaaat gacagggaga acatcatacc ggtctttcta
     1861 accttcaatg gcagtctgat gagtctgttt ataattgcag catatatttt tctggataag
     1921 gatgagggaa taatcaaggc ttatgctgta acggcatcct ccgtgtggca ctatctgctt
     1981 agcaagattg gagtaataat tattacatca atcgcaacca ccctgatggt aaccgttcca
     2041 ataatgggta ctcagccaaa ctatccagtg atgatactgt ttcttgtaac atcgggtttt
     2101 ttcgctgcat cactggggct ttttctcaca agctactata aggatattgc tcaggccttt
     2161 ggtgcccttt acattgtaat agtaatactc ataatgccaa acatatccta cttcacacca
     2221 agctgggatc ccgattgggt caagattatt ccaagctatg taatgctaca gagtttcaag
     2281 gagataatat cagtcaatgg taacatgtcc tatgtaatcc tggcatcttt gggctttgca
     2341 gtgctgggca tggatatatt cctgctcgca aactacagat tcaagaagac tcttaccctg
     2401 taaaggaagt gttgtgaaat gattaggaaa atacttttat tgttccagag ggatctgaag
     2461 gtcagcgtaa ggaatttcat aacactatat ataataatcg taccggttat gtttgccctt
     2521 atgatcaatg tattttctcc tggaatcaat gacaccacag ttgaaatagt ccttctggaa
     2581 ggtgaaaatc ctcagcagga ggaatacttc agccaatttg caaaggttga aactttaaag
     2641 actgtgacgg atgttgaaga gagggttctt aagagggaca acattgttgc tgtgcttcct
     2701 gacggaggcg atgggtactt catccttaga cagggtaatg agcctgacta cataattgac
     2761 tatgtaagaa atctcactgc ctttgaccat taccagattg gcattgagga ctccacagct
     2821 gaaatagttg attatggaag gaccatacca ccgcttaaga agataatggt caatgttgca
     2881 ttgattttta catccatcat gggtgggatg ataatagcaa ttaacatagt ggaggagaag
     2941 gttgacaata caattagtgc aatccatctg tcgccggtat caaggcttgg tttcattgcc
     3001 ggcaagagtc tcataggagt gtttgtacca atagttgggt catttattct tatgtttttc
     3061 actggcttca gggacataaa ctatttccac gcatttataa tggttgtcac aacctgcctc
     3121 atcagcctga cggtgggctt tgtcgaggga gtcaacaatg atgacatcat gactgcagca
     3181 gggaacatga agcttctttt catgcccttg tttgggagca tagcaggtgt ggaacttctt
     3241 gcagacaagt ggcagccact attctactgg attccttttt attgggcata caagggaaac
     3301 gatttggtac tcaccagttc aggtacatgg cttgatgtat tgaaatattc aggaattgtt
     3361 tttggcataa gcgtacttgt attcatatta cttgcaccta gaatcagaaa gggtcttgaa
     3421 taagggggcc tggacagata aagggtggta aattgtgaga gggaggtatt actatgagct
     3481 tatggggcgt actggcagtg gtttattctg cagcggttgt ggtgattact gtagtcaagc
     3541 cagaaaagat ctggaatatg aagaagattg aattgttcaa gaaggtattg ggggagaagg
     3601 gaacagaaat attcttttat atatgggccc tgatattcct ggtactggga atttggctaa
     3661 taaccaggta gaagaaccca aaaatgctct ccatctggag agcattttag tatcttgtct
     3721 ttaggatttc cttttcaatc tcaagaagct cattgattgt actctcacca agcttagaaa
     3781 aaagctccct tctcttctca gatgcctttt cctttttatc cacattcttt tccttcttga
     3841 atagggatgc ccactggacc ttctttccat tttcaaaaag gtcgtagtca attatcctcc
     3901 tgccatcatc caggtactca agtccaaaca ggattttctt aacctcctgg gaagagtaga
     3961 aggtaagaag cttgatgctg tagacgtcga tgaaaagcag ggcccttggc tggtcctttg
     4021 accatgattc ccctgtcagc aaacctatct gaagtgcgtc aaggaccgta agaagtctct
     4081 tgtagtggag ctcataggca ccaaagctgt catatatcct gatttcaaga tttgaccagc
     4141 ccttggaacc gactccaagg ttcagatact tgatggagtg tcttctaagc acctgcttta
     4201 cgttaaaata atcctggtcc aaccgaataa actgagggtg gaagtcatca gcagccatgg
     4261 agatgtagat gggtttttct gttattccaa taatcgtcct gtcttcaaat ccgttctctc
     4321 cgcattcgtc caagcttcca agctggcctc cgaaatcttc cattaccttg tcaagcacag
     4381 cgccaaggtt tccagcagat cttaggataa gcacctgcgg acctgacctc atggtttcta
     4441 tcaagccatg ggccaccatt gaacgtagtc tgcatgcaag cacaagggga tccataaata
     4501 caacaagtgc atcggtcttc ctgtattctg tcttagcctt gttttcatac tctatgatat
     4561 atactatggt atgattctct tcgctcctct tcatcactgt ttccatatca accagaactc
     4621 catcgagctc atcacacagg aatctgatca gctcgtcctt gtcagatgtg gatattagca
     4681 atgacctggg gccacttctt actaaagata ccataaaatc atcctcctta tttaagcctt
     4741 aaagcttgat tttccttctt ttgagtcttt cgttgtctgt gtcggcagga taatccaact
     4801 tgatcacctc cagggtttca agatcaatta tgtgtatagc tgaaatcccg ttaaggtata
     4861 ttttactgtc aatggtttga ctttttatgg agaggtcatg tccgtatagg tagacgtctg
     4921 catccaaatc cataagactt tctgagtaat ggctgatggc aaaggtctta tcccctatac
     4981 tcagaagcat cttatcctca acaacattga tcctgtttga attatttgat atgaagtcca
     5041 ggttatcgtg attgcccaag ctaagatata tacccttggc ctgagctgta tccaatatac
     5101 caagtatctt cttggcttca tgcttgtacc tggccatata gctttgatga aggccaagct
     5161 ttatattatc tgcaatatcc cctgtatgta caatgtagtc tgggcgaatt agtctgatca
     5221 ccctcttgat ttcagggtag attatacttg gagtatcgga tatgtgtaga aggactgttt
     5281 tatgtgtatc cataagttcc tggtccacat tgatccttcc aaggagcaag tttataaaac
     5341 gttttacctt attcatctaa aaacacctca attcaattat acatgaaaat gggtaaagga
     5401 gaataggaat taattggaac cagtggaatt tccaggaaaa ctgtagacta atgagtgttt
     5461 acgtaaaaat gttaatatgg tatgatatgg taagcggtat gtttttaaca atcacataat
     5521 cttgtatcac aaaaaatata ttcaaatgaa agggagttat ggaatgatta aacctaacat
     5581 gtcagcacac tttgattcaa gaaagccttc tgatgtaaga ctggctcaaa tgaagtacga
     5641 tgaaagggag aagaagccgg aagcggtaat taatgtagga attggaaatg tatccttgcc
     5701 tacaaaccc
//
LOCUS       DASRQF010000168         2455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1250191, whole genome shotgun sequence.
ACCESSION   DASRQF010000168 DASRQF010000000
VERSION     DASRQF010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250191"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VFD79_04620"
     CDS             complement(<1..264)
                     /locus_tag="VFD79_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002840521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HZH93365.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITHAGAESANYPFCTIEPNVGVVAV
                     PDERIYKLAEIYKSKKIVPTAIEFYDIAGLVAGASKGEGLGNKF"
     gene            493..1377
                     /locus_tag="VFD79_04625"
     CDS             493..1377
                     /locus_tag="VFD79_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZH93366.1"
                     /translation="MEWIKLSERITEIAGEGCEILVPFNERKPLKDIERSIIKTYRKH
                     IWTKFIKAVKEYELIQDGDKVAVAISGGKDSLLMAKLFQELHKYGTQNFELEFIAMDP
                     GYHPDIRDLLEDNCRYLDIPVHIYEANVFEVADKIAQDYPCYMCARMRRGALYGKAQE
                     LGCNKLALGHHYNDVIETTMLNVLCAGNFKTMMPKLKAQNFDDMELIRPMYLVEEKYI
                     ERFIQNSGIWPLNCACMVAAKKIGNKRTEIKDLIERLKDDFQNVDSSIFRAAQNVNME
                     GILGWEKDGEKHSFLDEY"
     gene            1477..2124
                     /locus_tag="VFD79_04630"
     CDS             1477..2124
                     /locus_tag="VFD79_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93367.1"
                     /translation="MLYTPQRFLKVLLGLFIFSVGIVLTINASLGVSPWDVFHQGVSR
                     TVGITIGQANIIMGFLIILLDIYLGQAIGWATVMNMLSIGTFIDILMLNNLIPVAQTP
                     AVGLIMIIAGVAIQAIGFSVYISQGIGAGPRDGLMVVLTKRTGKSVRLIKSVIEATAV
                     TIGIILGGSFGMGTIIMAFFGGIIFQATFRMIRFKVEKVEHRFIQDDLKALKNRA"
     gene            complement(2154..>2455)
                     /locus_tag="VFD79_04635"
     CDS             complement(2154..>2455)
                     /locus_tag="VFD79_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93368.1"
                     /translation="HWEEDSQEEKDIMERDFKSEIERIRGREIDIAFFPVDPRLGKHY
                     YLGGKYFIDNIKPKHFFPLHFGDNFEIIHEFINIPMENNTHIHEISKRNHIVDLI"
BASE COUNT          737 a          454 c          568 g          696 t
ORIGIN      
        1 aaacttgttt ccaagcccct cgcccttgct ggcacctgca acaagtcctg caatgtcgta
       61 gaactctatt gctgtgggaa cgatcttttt tgacttgtag atttcggcaa gcttgtagat
      121 tctctcatcc ggaaccgcta caacaccaac attgggctcg attgtacaga aggggtaatt
      181 cgccgactcc gctcctgcat gggtgattgc attaaataat gtactttttc ctacatttgg
      241 taggcctact attcctagct tcattttaaa ttcctttcta tatatgaatt attttcatca
      301 tctatccacg agttagaatt ataccctaaa tagctttgga ttgcaacgaa agtattgttt
      361 ctagaaagat tatatcacaa ggcatggcta ctgaggcacc aagtttacaa tagggccaat
      421 aatcttgtat taaatccata tttgttataa actagtaata ttagttagat acacaaagta
      481 atattatttt agatggagtg gattaagttg agtgaaagaa ttacggaaat agctggagaa
      541 ggctgtgaaa tactggttcc cttcaatgaa aggaagcctt taaaggacat agagagaagc
      601 attataaaga cctacagaaa gcatatctgg accaagttca tcaaggccgt aaaggaatat
      661 gagctgattc aggatggtga caaggtagct gtagcaatat cgggagggaa ggacagtctc
      721 ctgatggcca agcttttcca ggagctgcac aagtatggaa cacagaattt cgagcttgag
      781 ttcatagcaa tggatcctgg ctaccatccg gatatcaggg acctgcttga ggataactgc
      841 aggtatctgg acataccagt acacatctac gaagccaatg tatttgaggt tgcagacaag
      901 attgcccagg actatccctg ctacatgtgt gcaaggatga gaaggggagc actttatgga
      961 aaggcacagg agctgggctg caacaagctt gcccttggcc accactacaa tgacgtaatc
     1021 gagaccacca tgctcaatgt cctttgtgca ggaaacttca agaccatgat gccaaagctt
     1081 aaggcacaga attttgatga tatggagctg atcaggccaa tgtacctggt tgaggaaaag
     1141 tacattgaac ggttcattca aaactcagga atctggccac ttaattgtgc atgcatggtg
     1201 gctgctaaga agattggaaa caagaggacg gaaataaagg acctgataga aagacttaag
     1261 gatgatttcc agaatgttga cagctcaata ttcagagctg cacagaatgt caatatggaa
     1321 ggaattctcg gatgggagaa ggatggggaa aaacattcct ttctggatga atactaggaa
     1381 gcccaaggac taataaaaag tcctgtaaat ttaggttgaa aaatggcttt ggtctttttc
     1441 ggccgagtca aaatcaaatg gaaaagaagg ggtaatatgt tatatacacc gcaaagattc
     1501 ttgaaggtac tgcttggtct gttcattttt tcagtaggaa ttgtacttac aataaacgca
     1561 agtctgggag tttccccatg ggatgtattt caccagggtg tttcaaggac tgtaggcata
     1621 actattggcc aggccaacat aataatgggt tttctaataa ttctccttga tatttatctg
     1681 ggacaggcca taggctgggc aacggtcatg aacatgttgt ccatcggaac ctttattgat
     1741 attctcatgc tgaacaatct gattccggtg gcccagacac ctgctgtggg tctgatcatg
     1801 ataattgcag gagtggccat tcaggcaatt ggattctcag tttatatttc gcagggaata
     1861 ggtgcagggc caagggatgg tctgatggtt gttcttacga aaagaacggg aaaatccgtt
     1921 aggctgatca agagtgtcat agaggcaact gctgtaacaa taggaataat acttggaggc
     1981 agctttggaa tggggacaat aataatggcc tttttcggtg ggattatttt ccaggccacc
     2041 ttcagaatga ttagattcaa ggtggaaaag gttgagcaca gatttataca ggatgatctg
     2101 aaggcactga agaatagggc gtagaaaatg gatggctata aaagccatcc attttagatt
     2161 aggtcaacta tgtggttcct tttacttatt tcatggatat gtgtattgtt ttccatggga
     2221 atgttgataa attcatggat tatttcaaag ttatccccaa agtggagggg aaagaaatgc
     2281 ttgggtttta tattgtcgat aaagtatttt cctccaagat aataatgctt acccagtcga
     2341 gggtcaacag ggaaaaatgc aatgtcaatt tctctgcccc ttattctttc tatctcactc
     2401 ttaaaatccc tttccataat gtccttctct tcctgagaat cctcctccca gtgcc
//
LOCUS       DASRQF010000169         8305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1619961, whole genome shotgun sequence.
ACCESSION   DASRQF010000169 DASRQF010000000
VERSION     DASRQF010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 8305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8305
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619961"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1319
                     /locus_tag="VFD79_04640"
     CDS             <1..1319
                     /locus_tag="VFD79_04640"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HZH93369.1"
                     /translation="AIVSAVTLGLIAYGESGVFFAFGLSAVGILASIVGVFFVRGDGD
                     PQKSLDKGTLVSSLLTMIVGALLSIMILGSFQPFVAIIAGLVVGLTIAKITEYFTSAD
                     YNPVKRIAKESETGASTNIIGGLSVGMMSTALPIIVIAIGILVAYFASGGAAEPAKGL
                     YGIALAAVGMLSTAGMTIAVDAYGPIADNAGGIAEMCKLPEDVRKITDKLDSVGNTTA
                     AIGKGFAIGSAALTALALFSSYTQAVQLTAIDLTKPAVIAGLLIGGMLPFLFAALTMD
                     AVGKAAFSMIEEVRRQFREIPGIMEGTTTPEYATCVDISTKAALKEMIIPGLLAVVVP
                     VFIGLLLGAEALGGLLAGALVTGVLMAIFMSNAGGAWDNAKKYIEEGHHGGKGSEAHK
                     AAVTGDTVGDPFKDTSGPSLNILIKLMTIVSLVFAQMFVLYGGILF"
     gene            1431..2207
                     /locus_tag="VFD79_04645"
     CDS             1431..2207
                     /locus_tag="VFD79_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZH93370.1"
                     /translation="MENTIEIDGLKINYIKTGTGEPVLILHGWGASIETIMPIVNTLK
                     EDHTVFALDLPGFGKSQVPEEVFGSFLYADIVKEFMDRLIPGSLALVGHSFGGKLSII
                     LSSKYPERINRVVLIDSAGLIPKRTIKYHIRVKGFKLLKKLYLALVFWEEKDKRLEKL
                     YKRFGSDDYQNSSGVMRKILVRVVNENLKPILRDIKAPTLLIWGEKDEATPLYMGQIM
                     EKEISDSGLVVFEGAGHYSYLDDFNRFSRVMEAFFKSEDQ"
     gene            2238..3752
                     /gene="murF"
                     /locus_tag="VFD79_04650"
     CDS             2238..3752
                     /gene="murF"
                     /locus_tag="VFD79_04650"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019034661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HZH93371.1"
                     /translation="MILMIMTGFILAAAISVQLMSKRTKDSLHMVQLEGYLADHYLKW
                     LSTNKKKAYSLSGNEAPIKKPLVMTPRATRLYRANFLISATLVALPAILVNSISSNLV
                     AGLVVLLASMAAVYYYQPIVMALSTMAMKPVEDNINMGYYRAAQSKVKSRDNLEVVGI
                     TGSFGKTSTKFIVGTILSQKFNVLNTPESYNTPMGLSKVINNDLSDEHEVFVAEMGAK
                     TIGEIREVAQLAQPRIGILTSIGPVHMDTYKNIDNIMKTKYELIEELPTDGVAVFNYD
                     NEYIRKLADKTFKEKMLYGLENVEELDMYAEDIVVNEKGSEFTLRDREGNSVRCRTKL
                     LGRHNIYNLLAGACVSKILGLSFEEISMGIEKVEPVEHRLNLIESTSGIIVIDDAFNS
                     NPVGTRAALDVLSQFKEGRKIIVTPGMIELGEMEESANHEFGVNIGKVCDYVILVGEQ
                     RTKPIYEGLMDTSFNPSNIIVVNNLQEATEEIGKITKPKDVVLFENDLPDNYSE"
     gene            3769..4938
                     /locus_tag="VFD79_04655"
     CDS             3769..4938
                     /locus_tag="VFD79_04655"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HZH93372.1"
                     /translation="MKKVGVLFGGRSVEHEVSVITAMQIIENMDRTKYEPVAIYVTKE
                     GKWLTGKSLLSFESFKNNDFKDSKRIVISPNHNDGNLYAHPDETGLFGKKIVDTIDIF
                     FPAFHGTNGEDGAAQGLLEFVNKPYVGAGLLASSVGMDKIVMKDVYKANGIPVVDYIW
                     FYRSQWEADKEGSIDKVESKLGYPVFVKPSNLGSSIGISKAKNREMLQVALEIAASYD
                     RKIIVEKAVEEPREINCAVMGYNHEVEVSECEEPLGWSEILSFDDKYVRKDSKTSGSA
                     NKNRQIPANIEESTRLEIEELAKKAFTVIDARGNARIDFLIDGSGKVFVNEINTLPGS
                     IAFYLWEGKGYDFKALVNKMIDISLLTHNEKNSNMYVYESNLFNRTQFGSKTGKM"
     gene            5012..7144
                     /gene="rnr"
                     /locus_tag="VFD79_04660"
     CDS             5012..7144
                     /gene="rnr"
                     /locus_tag="VFD79_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HZH93373.1"
                     /translation="MAIREEIIKLMEGKKYKPLLKEELASRFNIGKGEQKEFFKVLEG
                     MEKEGVILKARNDRYGLVNPEFLVIGRLEGNEKGFGFVMSKDRPGEDIFIPADSINTA
                     MHGDKVIANLIKGRQAGRKQEGEIIRVLERANESVIGTYEDSGNFGFVIPDNHKIAYD
                     IFIPKGKTMKAKDGQKVVVGIEKWPEFRRNPEGSIIEVLGYLTDKGTDILSIIRQFKL
                     PEEFPDEVRAEARKIPSRIDGDEIKNRKDLRDLKTFTIDGADAKDLDDAISIEKLDNG
                     KYRLGVHIADVSHYVRPGSQLDKEALNRGNSVYLLDRVIPMLPHELSNGICSLNPNVD
                     RLTMSVFMDIDRNGKVVYHEIHESVINSKARLVYDDVSDLLEGTDEKAAEKLSIVADE
                     LFIMKELMDILRRHRDKRGSIDFDFPESYIELDENGKPVDVRKLERRVANRLIEEFML
                     VTNETVAERFFWEEIPFLYRIHEEPNSEKIEDFQKFIHNFGYAIKGKELHPKDFQILT
                     QEIKGKREEPVISTLLLRTMQKAVYSAEPGIHFGLAAQYYSHFTSPIRRYPDLEIHRI
                     IKDSIKGKIGEKKMNMLEGLLPEIAEHTSMTERRAEEAEREVDDLKKAQYMAERIGEE
                     YEGLVSSVTSFGLFVQLENTIEGLVHFSNMVDDFYFFDEDNYMIVGERTKTTYRLGDP
                     VKVKVIGADVGKKNIDFQLVNDEVDTDE"
     gene            7190..7654
                     /gene="smpB"
                     /locus_tag="VFD79_04665"
     CDS             7190..7654
                     /gene="smpB"
                     /locus_tag="VFD79_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HZH93374.1"
                     /translation="MKKTGTKLIASNKKARHEYFIEDTLEAGIVLTGTEVKSIRQGKV
                     NIKESYASIENGEVFITGMHISPYEQGNIHNVDPIRKRKLLLHRREIRKLIGETQQKG
                     FTLVPLSIYLKDGLVKVELALAKGKKLFDKRQTMAKKDAERRIQQHSSEKYQ"
     gene            7711..8061
                     /gene="ssrA"
                     /locus_tag="VFD79_04670"
     tmRNA           7711..8061
                     /gene="ssrA"
                     /locus_tag="VFD79_04670"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         2697 a         1529 c         2034 g         2045 t
ORIGIN      
        1 gtgcaattgt atcagctgtt acactaggac ttatagctta tggggaaagt ggagttttct
       61 ttgcatttgg cctatcagcg gtgggaattc ttgcttcaat agttggagtt ttctttgtaa
      121 ggggagatgg agatccacag aagtcactgg ataagggtac acttgtcagc tcattgttga
      181 caatgatagt aggagcctta cttagcatca tgattctagg atcattccaa ccatttgttg
      241 caatcatagc aggtcttgtt gttggactta caattgcaaa gattacagaa tactttacat
      301 cagctgatta taatcctgtt aaaaggattg ctaaagaatc agaaacggga gcatcaacaa
      361 atatcattgg tggactatct gtaggaatga tgtcaacagc acttccaata atagtaattg
      421 caataggtat ccttgtggca tactttgcat caggtggagc ggcggagcca gccaaaggac
      481 tttatggaat cgctcttgca gctgtaggaa tgctttcaac agctggtatg accatagcag
      541 tcgatgctta cgggccaatt gcagacaatg ctggtggaat cgctgaaatg tgtaagctac
      601 ctgaagatgt tagaaagatt accgacaagc ttgactcagt tggtaataca acagcagcaa
      661 tcggaaaagg atttgcaatc ggatcagcgg cacttactgc tctggctctt ttctcatcat
      721 acacgcaagc tgttcaactt acagctatag accttaccaa gccagctgta atagcaggtc
      781 ttctaatcgg aggaatgctt ccattcctat tcgcagccct aacaatggac gctgttggaa
      841 aagctgcatt cagcatgatt gaagaagtaa gaagacaatt cagggaaatt cctggaatca
      901 tggaaggaac cacaactcct gagtatgcta catgtgttga catcagtaca aaagctgctc
      961 ttaaggaaat gatcatacca ggattacttg cagttgtagt gcctgtattc ataggattgc
     1021 ttctgggagc agaggccctt ggaggacttc ttgcaggagc acttgtaacg ggagtactga
     1081 tggcgatctt tatgtcaaat gccggaggag catgggacaa cgccaagaag tatattgaag
     1141 agggacatca cggtggaaaa ggtagcgagg cccacaaggc tgctgttact ggagacacag
     1201 taggtgaccc attcaaggat acatcaggtc cttcattgaa catcctgata aagcttatga
     1261 caatcgtatc acttgtattt gcgcagatgt ttgtactata cggtggaata ttgttctaga
     1321 tagtgaccct ccttatggag ggttttttct tgcctggaag actgtttaaa ttccaattaa
     1381 tatgatataa ttttatgttg taacacagtt aaaaagaagg atggagcaga atggaaaaca
     1441 ctattgaaat agatggtctc aaaatcaact acataaagac cggcacagga gaaccagttt
     1501 tgattctcca tggctgggga gcaagcattg agacgataat gccaattgta aataccctta
     1561 aggaggacca cactgtattt gctcttgacc tacctggatt cgggaaatcc caggtgcctg
     1621 aggaagtgtt tgggtccttc ctatacgcag atattgtaaa ggagtttatg gatagattga
     1681 ttccaggaag tctggctctt gttggacatt cctttggggg aaagctatca ataatacttt
     1741 catcaaaata tccggaacgg atcaataggg ttgtattgat tgacagtgca ggacttatac
     1801 caaaaaggac cattaagtac cacattaggg taaagggctt taagcttctc aaaaagcttt
     1861 acttggccct tgtgttctgg gaggaaaagg ataagaggct ggaaaagcta tacaagagat
     1921 ttggctcgga cgactatcag aacagttcag gtgtaatgag aaaaatactt gttagggttg
     1981 tcaatgaaaa cctaaaacca atcctaagag acattaaggc acctaccctg ctgatctggg
     2041 gtgaaaagga cgaagcaacc cccctgtaca tgggacaaat aatggaaaag gaaatcagtg
     2101 acagtggcct tgtggtattt gaaggggcag gccattattc ctacctggac gacttcaata
     2161 gattttccag ggttatggaa gcctttttca agtcagaaga ccaataagca tttggcaaac
     2221 atatttggag gaaaataatg attttaatga taatgactgg atttattttg gctgcagcca
     2281 tatctgtgca gcttatgagc aagagaacaa aggattcact tcatatggta cagctggagg
     2341 gatatttggc agaccactat ctgaagtggc tttcaacaaa caagaaaaag gcatacagcc
     2401 tttcaggaaa tgaagcacca atcaagaagc cattggttat gacaccaagg gctacaaggt
     2461 tgtacagggc aaatttcctc atatcagcta ccttggttgc cctacctgca atacttgtca
     2521 actcaatttc cagcaacctg gtcgctggac ttgttgttct tcttgcatca atggctgctg
     2581 tatactacta ccaaccaatt gtcatggctc tttccacaat ggcaatgaaa ccagtggagg
     2641 acaatatcaa tatgggctac tacagggctg cccaaagcaa ggtgaagtca agggataatc
     2701 tagaggtggt tggaataacg ggaagcttcg ggaaaacaag caccaagttt atcgtgggaa
     2761 ctattctctc tcagaagttc aacgtcctaa acactcctga gtcctacaat acacccatgg
     2821 ggctttccaa ggtaataaac aacgacctct ccgatgagca tgaggtcttt gtagctgaaa
     2881 tgggagcaaa gaccataggt gaaataagag aggttgccca gcttgctcag ccgagaatag
     2941 gcatactgac atcaatcggc cctgttcaca tggacacata taagaacatt gacaacatta
     3001 tgaagacaaa atatgaactg atagaggaac taccaaccga tggtgttgct gtcttcaact
     3061 acgacaacga atatatcagg aagctggccg acaagacctt caaggagaag atgctttacg
     3121 gtcttgaaaa tgttgaagaa ctggacatgt atgcagagga tattgtcgtt aacgaaaagg
     3181 gctctgaatt caccctcagg gacagggaag ggaatagtgt aaggtgcaga acaaagcttt
     3241 tggggaggca caatatttac aacctccttg caggtgcatg tgtatccaag atactgggcc
     3301 tatcctttga ggaaatcagc atgggaatag aaaaggtgga gcctgtagag caccggttaa
     3361 acctcattga atcaaccagt ggtataatag tgattgacga tgcattcaat tcgaatccag
     3421 tgggcactag agcagccctt gacgtacttt cacagtttaa ggaaggcaga aaaatcatag
     3481 tgactcctgg gatgatagag ctaggtgaaa tggaagagtc ggcaaatcat gagtttgggg
     3541 taaatattgg aaaagtatgt gattatgtca ttctggtagg cgaacagaga actaagccaa
     3601 tctatgaggg gctgatggac acatcattca atccatccaa tataattgta gtcaataatc
     3661 tacaggaagc cactgaggaa attgggaaaa taaccaagcc aaaggacgta gtgttgtttg
     3721 aaaatgatct accggacaat tacagcgaat agcaagagga ggttgacaat gaaaaaagta
     3781 ggagttcttt ttggaggcag aagtgttgag cacgaggtat ctgtaataac tgcaatgcag
     3841 ataattgaaa atatggacag gaccaaatat gaacctgtag caatctatgt caccaaggag
     3901 ggaaaatggt taacaggaaa atccctatta agctttgaga gcttcaagaa caatgatttt
     3961 aaggattcca agagaattgt gatttcacca aatcacaatg acggcaacct ttatgcccat
     4021 ccagacgaga ctggactgtt cggtaagaag atagtcgata caattgatat tttcttccct
     4081 gcttttcatg gaacaaatgg tgaagatgga gctgcacaag gcttgcttga gttcgttaac
     4141 aagccctatg tgggtgcagg tcttcttgca tcatctgtag gtatggataa gatagtgatg
     4201 aaggatgtat acaaggccaa tgggatacca gttgtggact atatctggtt ctacagaagc
     4261 cagtgggaag ctgacaagga aggatccatt gacaaggtgg aatcaaagct tggatatcca
     4321 gtctttgtaa agccttcaaa ccttggttca agcataggta tttccaaagc caagaacagg
     4381 gaaatgcttc aggttgcctt ggagattgca gcaagctacg acaggaagat catagtggag
     4441 aaggcagtag aggagcctag agaaataaac tgtgcagtta tgggctacaa ccatgaggtg
     4501 gaggtttctg aatgcgagga gccgctggga tggagcgaaa tcctatcatt cgatgacaaa
     4561 tatgtaagaa aggattccaa gacatcagga tccgccaaca agaatagaca aataccagca
     4621 aacattgaag aaagcaccag gcttgaaata gaggagcttg caaaaaaggc cttcacagtg
     4681 atagatgctc ggggcaatgc caggatagat ttcctgatag atggcagcgg taaggttttt
     4741 gtaaatgaaa tcaataccct tcccggttca atagctttct atctttggga aggaaaaggc
     4801 tatgacttca aggccctggt aaataagatg attgatatct ccctattgac acataatgag
     4861 aagaactcca acatgtatgt ttatgagtca aacctattca acagaaccca atttggctca
     4921 aaaacaggta aaatgtaaga tattttatat atcttacatt ttttttaaca gaaggggtaa
     4981 atactaggta ttgaaagaag ggggatttac aatggcaatt agggaagaaa taataaagct
     5041 tatggagggg aagaaataca agcccttact taaggaagag cttgcatcaa gattcaatat
     5101 tggtaagggc gagcagaagg aattcttcaa ggtattggaa gggatggaaa aagaaggtgt
     5161 gattctaaag gcaagaaacg acagatatgg tcttgtcaat cctgagttcc ttgtaatagg
     5221 aaggcttgag ggtaacgaaa agggctttgg atttgtaatg tcaaaggata ggccaggtga
     5281 ggacatcttc attcctgcag actccattaa cactgctatg cacggggaca aggtaattgc
     5341 caaccttata aagggtagac aggcagggag aaagcaggag ggtgaaataa tcagggtgct
     5401 tgaaagggcc aacgaatctg ttattggaac ctatgaggac agtggtaatt ttggatttgt
     5461 aatacctgat aaccacaaga ttgcatatga tattttcatt cccaagggaa aaaccatgaa
     5521 ggccaaggat ggacaaaagg ttgtcgtagg tattgaaaag tggcccgagt tcagaagaaa
     5581 tccagaaggt tccatcattg aagtcctggg ctatcttact gacaagggaa cggacatcct
     5641 atcaataata agacaattca agcttccaga ggagtttcct gatgaggtca gagccgaggc
     5701 acgtaagatt ccttcaagaa tagatgggga tgaaatcaag aatagaaagg atctgaggga
     5761 cttaaagaca tttacaatag atggagcaga tgcaaaagac cttgatgatg caatatccat
     5821 agaaaagctg gacaatggaa aatacaggct gggggttcac attgctgatg tatcacatta
     5881 tgtaagacct ggaagccagc tggataagga agccttgaac agaggtaatt cagtgtattt
     5941 actggacagg gtaataccaa tgcttccaca tgagctaagc aatggaatct gttccttaaa
     6001 tccaaatgtt gacaggctga caatgtcagt ctttatggat atagacagaa atggtaaagt
     6061 agtctatcac gagatccatg aatcggttat caacagtaag gcaaggcttg tatacgatga
     6121 tgtttcagat cttcttgagg ggacggatga aaaggcggca gagaaactat caatagtggc
     6181 ggatgaacta tttatcatga aggaattgat ggatatatta agaagacaca gggacaagcg
     6241 gggcagtatt gactttgatt ttcctgagag ctacattgag cttgatgaaa acgggaagcc
     6301 agtcgatgta agaaagcttg agagaagggt tgccaacaga cttatcgagg agttcatgct
     6361 tgtcaccaac gagaccgtgg cagaacgttt cttctgggag gagattccct tcctgtacag
     6421 aatccatgaa gagcccaata gcgagaaaat tgaggacttc caaaaattca tccacaactt
     6481 tggctatgcc ataaagggaa aagaactgca tccaaaggac tttcagattc ttacccagga
     6541 aattaagggc aagagagagg agcctgttat atcaacacta ctccttagaa ccatgcaaaa
     6601 agccgtttac agcgctgagc caggaatcca ctttggactg gctgcccagt actactcaca
     6661 cttcacatct ccaatcagaa ggtaccctga ccttgagata cacaggataa taaaggactc
     6721 aatcaaggga aaaataggtg agaagaagat gaatatgctg gaaggactac ttcctgagat
     6781 agccgagcac acttccatga ctgagagaag ggctgaggag gccgagaggg aggtcgatga
     6841 ccttaagaag gcccagtata tggctgagag gattggagag gaatacgagg gtctggtgtc
     6901 ctctgtaacc agctttggat tgttcgtcca gcttgaaaac acaatcgagg gacttgtcca
     6961 cttcagcaac atggtggatg acttctactt cttcgacgag gacaactaca tgatagtggg
     7021 cgagaggaca aaaacaacat atcgacttgg agatcctgtc aaggtgaagg tcattggagc
     7081 agatgtagga aagaagaata ttgacttcca gcttgtaaat gacgaagttg acacagatga
     7141 gtaattttgc taaactgaaa aacgacaaaa gataaaggga tggaatcaaa tgaagaaaac
     7201 aggcacaaag ctaattgctt caaataaaaa ggcaaggcac gaatatttta tagaggatac
     7261 acttgaggcc gggattgtcc ttacaggaac tgaggtaaag tccataaggc aggggaaggt
     7321 caacatcaag gaatcctacg cttccattga aaatggagag gtattcataa cagggatgca
     7381 cataagcccc tacgagcagg ggaacataca caatgttgac ccgatcagaa aaaggaagct
     7441 ccttctccac agaagggaga tcagaaaatt gattggtgaa acccagcaga agggtttcac
     7501 ccttgtgcct ctttcaatat acctgaagga tggccttgta aaggttgagc ttgctcttgc
     7561 caaaggtaag aagctgttcg acaagagaca gaccatggcc aagaaggatg ctgagcgtag
     7621 gatacagcag cattccagtg aaaaatatca gtagccaggg gattgacaca aagtcgaaca
     7681 tatgttataa ttcatatgta ataaccaaga gggggcgaat ttggtctcga cggggatacg
     7741 gaatcaggag tagcgagccg ttgtcgccaa gcaacgataa aagtggcaaa caaaattaaa
     7801 cgcaaacgat aattacgcac tagcagccta ataaggccgc tcgatttaac cgggagtccc
     7861 acgacctgga ttgaatcgtc attttagtgg ggaaccgagt ccggtaaagc tttgaaccgg
     7921 aaacggaact gatgaagcta ccgaagctca tatccagtca gcaggagatg agtcggggga
     7981 atcctaaatt gttggctgcg ctcggagaag ctcttgtgga aggattttcg gacgtgagtt
     8041 cgactctcac cgcctccacc ataattaatg aaagaaaccc ccaggctagc ctgggggttt
     8101 aaatttctgg aaatataatt accttaaggc cattcactga ccaaaatatc atactcaggt
     8161 atgacaaaga ttatcataca tcctgaagaa caattcgatt tttgatttta cactatcatc
     8221 accagcctgg tctagaagag ctttcctttc tttttccacg tcctggaata ccgacttgtt
     8281 tctaaataca tatagtgtat caaga
//
LOCUS       DASRQF010000170        12006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_132192, whole genome shotgun sequence.
ACCESSION   DASRQF010000170 DASRQF010000000
VERSION     DASRQF010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 12006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12006
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132192"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="VFD79_04675"
     CDS             complement(<1..901)
                     /locus_tag="VFD79_04675"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HZH93375.1"
                     /translation="MTKRIRFFDTTLRDGEQSPGCSMNHREKLMMARQLEKLGVDVIE
                     AGFAAASPGDFQSVKAIADQITESTVAVLSRTVKEDIDKAWEAVKGAAKPRIHTFIAT
                     SPIHMEYKLKMTPQQVADRAEEMVAYAASLCPEVEFSAEDATRSDTDFLAEIFDRVIQ
                     AGAKIINIPDTVGYTTPDEFYQFIMDIRRKSKYLDGVEISVHCHNDLGLGVANSLAAI
                     KAGATQIECTVNGIGERAGNAALEEIAMVLSVRGDKYGANSNINTVEITRTSNMLSSI
                     TGVNVQPNKAIVGANAFAHESGIH"
     misc_binding    complement(1051..1287)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1349..2347)
                     /locus_tag="VFD79_04680"
     CDS             complement(1349..2347)
                     /locus_tag="VFD79_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HZH93376.1"
                     /translation="MKIGLVGTGGVGRALIGLIGKRDDMDLVFALNSRSGAGDPSGLD
                     CEGLAEYLQENSDLSEYGEGGFGTWELGDVLKSYKVDLLVEATPTNKGTGEPAATTIR
                     LALQEGIHVVTANKGPVMLHFKELGDLAEENGLGLGIGATAGAALPTISAGENDLLGS
                     RLLGIRGVLNGTTNYILHLMETEGMSYGDALRKAQEEGIAETDPSQDVEGWDTAIKMT
                     IIANALAGTDLTLEDAQVTGISHLTLEDIGAAKREGKRYRLLGELDLQDENPRALVRP
                     IMVSQEDIFYGVQGKNKGVEYITDNLGEIAILGGASDVRGAAAAIIRDILQIKKNF"
     gene            complement(2358..3230)
                     /locus_tag="VFD79_04685"
     CDS             complement(2358..3230)
                     /locus_tag="VFD79_04685"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZH93377.1"
                     /translation="MFEKLKGYIAVIISAIVFGSMPLMAKTVYQEGGNPVTLSFLRFF
                     LVLPVLAYFVNRNKKVGLGLTREEAKKLFLVGTLGYGTTALTLYMSYDYIASGIATTL
                     HFAYPVLVILVYVILFKERASSIKMLSVSLTIVGIMFLNDSEANMNLMGFLLAFGSSV
                     AYAFYIVYLDRSGLKSMNSLKLTLYLCIMASGMIFLFGLFTGTLDFNMTPLGWGISTL
                     LSILVAFLGVSFFQIGVALIGPQKTSILSTFEPVTSIIIGALLFNELISGRTIAGFAS
                     ILAAVVIITAFDRD"
     gene            complement(3363..4016)
                     /locus_tag="VFD79_04690"
     CDS             complement(3363..4016)
                     /locus_tag="VFD79_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93378.1"
                     /translation="MSQLKSRVKLPSGIKKINLENVVVRKAVEKDIEGIFHVAASVGK
                     KSQDPMQGFLMDDYGSDPEGHKNKLKRALEISDYFYVAEYIEDDYRSVLGFTFGLPKD
                     VWLGENPGWLDDCYFRPDFDRESLDNFIMLDKIAVLDRFKRQGIGGLLSRRLIRDIKA
                     DGMFDIFEEVIIAPIPNLPSVLFKTKRKFKLASVRYEWHEGKVMTTLVYHRRLRRDT"
     gene            complement(4126..5265)
                     /locus_tag="VFD79_04695"
     CDS             complement(4126..5265)
                     /locus_tag="VFD79_04695"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HZH93379.1"
                     /translation="MKILIASDSFKGSATTMEVAERIEKGLLRIIPELEVLKLPMADG
                     GEGTVETLVYGMDGKFVCEEVTGPLGDRVQAKYGIIQDQIAIIEMAEASGLVLVEEER
                     RNPSLTSTYGTGELILSALQRGYGKIYVGIGGSATNDGGVGMARALGYRFLDRDGNEI
                     PEGGGGLDGLRRIDRSNVTPLLEDAQITVMCDVENPLYGPGGAAYVYGPQKGADPQMV
                     QLLDRNLRHLADILKEDMDVDIGDVPGSGAAGGLGGGLMAFCGADLRPGIEMVLDILE
                     FDGLLKDVDLVITGEGRIDGQSSKGKVPAGVAKRARKLNKPLVAMAGGVGEGTEILHT
                     MGIDLILPIVDRPMGLLDAMDGVGILLEKTGERLGRIIRLADKLK"
     gene            complement(5262..6152)
                     /locus_tag="VFD79_04700"
     CDS             complement(5262..6152)
                     /locus_tag="VFD79_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZH93380.1"
                     /translation="MGVFLMLLSSLFFTLSTFFGKLVSNTTEMNALVTSFVRYFLGTV
                     GLGAFMVYRGISFKPVRKKPVFIRSALNASALILLTASLNYTTITNANMIHLTYPVFV
                     FLLARFITGESLRPSAFIYLFLILVGSYIVSDPHLGSINIGDLISFGSAFFAGTSILY
                     LTQARRTESSIIIVFYLMLIGMFLNFPLVIDDIGILSMDSAGIVLLAAGTGFLGQVFT
                     TEGFKYVDSATGSMLSSSRMVMAALIGYFFLAEPLNLRIVTGILLISIALFGVSGYGG
                     VLREKYRYGRGMEKSQEAEK"
     gene            complement(6239..6709)
                     /locus_tag="VFD79_04705"
     CDS             complement(6239..6709)
                     /locus_tag="VFD79_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93381.1"
                     /translation="MDIKKTIKDIADNHHGFLKEELPVIKSLAYKILKVHYHDCKEEL
                     IQVHRQYGRVQNELELALVKKQMVLLPMIWDYQKEPSKELLEEMERTIQEVEGDNDIL
                     LGELEGLKEKTNTYTMPESGCPTYESTYRKMEKLHQEVERYIGIEREMYKDLLG"
     gene            complement(6767..7237)
                     /locus_tag="VFD79_04710"
     CDS             complement(6767..7237)
                     /locus_tag="VFD79_04710"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZH93382.1"
                     /translation="MYRKELISYNYATRMGRKIEYIVLHDTGNPRKGADAYAHYRYFN
                     HPGRNASAHYFVDAGETIQLVEDFNASWHCGDGRGRHGITNSNSIGVELCVNSDGDWE
                     ETKRRGILLVRDLMNKHGIPKERVVRHFDASGKVCPRRMSDWGWREWTLFYDKI"
     gene            complement(7230..7796)
                     /locus_tag="VFD79_04715"
     CDS             complement(7230..7796)
                     /locus_tag="VFD79_04715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZH93383.1"
                     /translation="MERTIQEIYMDVASGREDAGLELLMRLQPLIISSIKKYYNKTED
                     MEDLVQEGRLLVLQSIGDYREDYSVPYLGFIKSRLKYLYLGKNRNRRILSLNVQVGED
                     GEDHMDLLEDGFKLEEDYIRREQGSELLQMISRLPLRERQVVQGYYLENLSIGDISKR
                     HGITYRTVVNTKRRALERMRKEMEEEHV"
     gene            complement(7897..8481)
                     /locus_tag="VFD79_04720"
     CDS             complement(7897..8481)
                     /locus_tag="VFD79_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93384.1"
                     /translation="MKELMKEDILVMIPSYEGESGNCTRVYLKENMVVVSSTLRTFLR
                     KLCSYHHFDLKASNNHYGKLISARTNLPIPIDSRTIYIQLKVREPIGKDDGAMGYFKL
                     GGIRKIFASEGVTFVRMENGVDHPCLCKLETAKKQLRQGNLVRELMEKKRSQLVGEAI
                     EYYTSQDGPAMKSDIARLYMKIDALLLKMENGYE"
     gene            complement(8644..9873)
                     /locus_tag="VFD79_04725"
     CDS             complement(8644..9873)
                     /locus_tag="VFD79_04725"
                     /inference="COORDINATES: protein motif:HMM:NF006771.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HZH93385.1"
                     /translation="MKINIERVKRDINNLASFSSTPGRGVTRLAFSKEDVQAKGYLMA
                     QMEAAGLEVYLDGYGSLFGRREGTEPDLPPVMIGSHYDTGIGGGPFDGVAGIVAGIEI
                     ARTLKENGASHRHPIEVAAFNDEEGARFGNAMSNTRAMAGFLTQEELDRTKDINGKPL
                     REAMEECGIKVDLDLAKREKGSLKAFLELHIEQGPVLEKAGMDVGIVDTIVGLDRYEV
                     KFRGLAGHAGITPMAGRKDALVSAAKFIVAVNEIARETGKGAVATVGEMSIYPNASNL
                     IPELVKLSVDIRATDSQLINTMSDKVQKELEKIGYADPVEIEFKRGVYIPPVEMDRDN
                     GDLLRRKAEDLGLKYRVMNSGAAHDAMVMAALAPANIIFVPSVGGLSHDPEEWTEYDD
                     LENGIQLMLQTITELAG"
     gene            complement(9897..11144)
                     /locus_tag="VFD79_04730"
     CDS             complement(9897..11144)
                     /locus_tag="VFD79_04730"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF002823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HZH93386.1"
                     /translation="MDIINDLKSMREVYWTNPGYRKFDDIRDELPITEEEVMEAEKRL
                     RRFAPYLAKVFPESKDGIIESPISHIPEMMSWIERDSGSTIPGELYLKRDDLLSIAGS
                     IKARGGIYEVLKHAETLAVENGMLQYDHDYRKLAEREFVDFFSRYKIQVGSTGNLGLS
                     IGIMSAKLGFKVTVHMSSDAKQWKKDMLRSKGVKVVEYPDDYSKAVEEGRRDSDSDPM
                     SHFVDDENSRDLFLGYAVGGMRVKSQLEEMGVIIDERHPLVVYLPCGVGGGPGGVAYG
                     LKLSYGDLVECHFAEPTHSPCMLLGLATGKHDGISVEDIGLDNRTEADGLAVGRPSSF
                     VGRLMDKLLTGAFTIQDDKLFKYLKALYGQEGIFVEPSATAGFNGLFLTKGRYIDRDP
                     VQLVWSTGGSLVPKEERSKFLGE"
     gene            complement(11483..11767)
                     /locus_tag="VFD79_04735"
     CDS             complement(11483..11767)
                     /locus_tag="VFD79_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004837488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HZH93387.1"
                     /translation="MYYVYILANWNNKVLYTGVTGNLQRRIYEHKNKLADGFTNKYNV
                     EKLVYYDSTDDVREAIKREKEIKGWKRIKKDDLVSEFNPEWRDLSEDIYR"
BASE COUNT         2923 a         3288 c         2330 g         3465 t
ORIGIN      
        1 ggtggattcc cgactcatgg gcaaatgcat tggctcccac aattgccttg ttgggctgga
       61 cgtttacacc ggtgatgctg cttagcatat tgcttgtcct ggtgatctcg acggtattga
      121 tattggagtt tgcaccgtac ttgtcccctc ttactgaaag gaccattgct atttcctcaa
      181 gggctgcgtt gccggccctt tcacctatcc cattaactgt gcactctatt tgtgtcgccc
      241 ctgcctttat ggctgcaagg ctgtttgcaa ctcccagacc caaatcattg tggcagtgga
      301 ctgatatttc caccccgtcc agatacttgg attttcttct gatgtccatg ataaactggt
      361 agaactcatc cggtgttgtg tagcccacgg tgtctggtat gttgatgatt ttagctcccg
      421 cctggatcac cctgtcaaat atttcagcaa gaaagtcagt gtcgcttctc gtggcatcct
      481 cagcagaaaa ttcaacctcc gggcaaaggc tggctgcata tgccaccatc tcttcagccc
      541 tgtcagcaac ctgctgtgga gtcatcttca gcttgtattc catatgtatt ggtgatgtgg
      601 caataaaggt gtgaatccta ggctttgccg ccccctttac agcctcccag gccttgtcga
      661 tgtcctcctt gactgtcctt gaaaggaccg ccaccgttga ttcagttatc tggtccgcta
      721 tggcctttac agattgaaag tcaccaggtg atgcagctgc aaagcctgcc tcaatgacat
      781 ccactcccag cttctccaat tgccttgcca tcataagctt ttccctgtga ttcatgctgc
      841 accctgggga ctgctccccg tcccttaatg tagtgtcaaa aaacctgatc ctctttgtca
      901 tttgaatccc ccttttctga ataaaaaaag catctgaatt gctctaatgt agctttttat
      961 gatactcatg aacgaaaatt tctagaattt atgtagtaga aattttctat tcgtaataaa
     1021 aaaaatcccc cgtccctata gatattaatc tatagggacg aaggataaat ctttcgtgtt
     1081 accaccctaa ttcgcagcaa gctgcctcat tcccggacta tcatccggta tccatataac
     1141 ggtggcaccg tcacagacta cttgccgaag ctttcgccca tgctgctttg aagctagtat
     1201 tacataccct tttactgctg attctcacca accatcagct ctcttgaagt aaaaccccgg
     1261 tatctttctc ttcatcttgg catttataat attgagacct attatatatc catggggatt
     1321 cataagtcaa cacaaaattt atacttttct aaaaattctt tttaatctgt agaatatccc
     1381 ttattatggc tgcagcagca ccccttacat ctgaggctcc tccaaggatt gcaatctctc
     1441 ccagattgtc ggtgatatac tccacgccct tgttctttcc ctgtaccccg tagaatatat
     1501 cctcctggga caccatgatg ggccttacca aagcccttgg gttctcatcc tgaaggtcca
     1561 gttcaccaag aagcctgtac ctttttcctt cccttttggc ggcccctata tcctccaagg
     1621 tcaggtggct tataccggta acctgggcat cctccagggt aaggtctgtt cctgcaaggg
     1681 cattggcaat tatggtcatc tttattgctg tatcccagcc ttcaacatcc tgtgatggat
     1741 ccgtctctgc aatcccttcc tcctgtgctt ttctcagggc atccccgtag gacatccctt
     1801 cagtttccat caggtggagt atgtagtttg tggtcccatt caagaccccc ctgattccca
     1861 gcagccttga acccagaagg tcgttctcgc cggcgctgat ggtgggaagg gcagcccctg
     1921 ctgttgcccc tattccaagg ccaagtccat tttcttcagc caggtcccca agctccttga
     1981 aatgcagcat aaccggtccc ttgttggccg tcacaacgtg aatgccctct tgaagggcaa
     2041 gcctgattgt tgtggcagca ggctcccctg tccccttatt ggtgggggtt gcttcaacca
     2101 aaaggtcaac cttgtagctc tttaggacat cgccaagctc ccaggttcca aagccccctt
     2161 caccatattc tgaaaggtca ctgttctctt ggagatattc agccagacct tcacaatcca
     2221 ggcctgaagg gtccccagct cccgatctgg aattaagcgc aaatacaagg tccatgtcat
     2281 cccgcttgcc aataagacca ataagtgccc ttccaacacc acctgtaccc actaaaccta
     2341 ttttcacaat acccctccta atccctgtca aatgctgtaa taataacgac cgcagccaag
     2401 attgaagcaa aaccggcgat tgttctcccg cttatcaact cattaaagag caaggctccg
     2461 atgattatgc ttgtcacagg ctcaaatgtg ctaagtatgg atgtcttttg gggcccgata
     2521 agtgccactc ctatctggaa aaagcttact ccaagaaagg ccacaagtat ggacagaagg
     2581 gtggatattc cccatccaag gggtgtcatg ttgaaatcca aggtccctgt aaacaaacca
     2641 aagagaaata tcatcccgct tgccattatg caaagataaa gggtgagctt cagggagttc
     2701 atggacttca gaccacttct gtcaaggtag actatgtaga aggcataggc cacagatgag
     2761 ccaaatgcca gaagaaatcc cattagattc atgtttgctt cagagtcatt gagaaacatt
     2821 atccccacaa tcgtcaggct cactgatagc atcttgatac tgctggccct ttccttaaaa
     2881 aggatcacat acaccaggat cacaaggacc gggtatgcaa agtggagtgt ggtggctatt
     2941 ccagatgcta tgtagtcata tgacatgtac agggtcaggg ctgttgtccc atagccaagg
     3001 gttcccacca gaaatagctt cttggcctct tccctcgtta gcccaagtcc caccttcttg
     3061 ttcctgttta caaagtatgc caggactgga agaaccagaa aaaacctcaa aaaagataga
     3121 gttacaggat tccctccctc ctggtagacg gtctttgcca tgagcggcat gcttccaaac
     3181 acaattgccg atataattac tgcaatatat cccttcagct tttcaaacat cttgtcctcc
     3241 acaattaagt cattaaatag attataaccc ttatgtcgaa atattgcatg ggatttaagg
     3301 tcaaaacaaa aaaaagaaaa tccccagggt taaatcccgg ggacctgaca tgggcatctc
     3361 tcctaagtat ctcttctaag ccttctgtgg taaacaaggg ttgtcattac cttgccctca
     3421 tgccactcat accttacact ggccagcttg aacttacgct ttgtcttgaa aagaaccgat
     3481 ggtagatttg gtatgggtgc aattataacc tcctcgaaga tgtcaaacat tccatcagcc
     3541 ttgatgtccc tgattagcct tctggaaagc aagccaccta ttccctgcct tttgaacctg
     3601 tcaaggactg ctatcttgtc aagcatgata aagttgtcca ggctctcccg gtcaaagtcc
     3661 ggcctgaagt agcagtcatc aagccacccc gggttttcac ccagccacac gtcctttgga
     3721 agtccgaagg taaatccaag aacagacctg tagtcgtcct ctatgtattc cgccacatag
     3781 aagtaatcgc tgatttccag ggctctcttc agtttgttct tgtgcccctc cggatcagaa
     3841 ccataatcat ccataagaaa tccctgcatt gggtcctggc tcttcttccc cacagatgct
     3901 gctacgtgga atattccctc aatatccttt tcaacggcct ttctgaccac aacattttcc
     3961 agattaattt tcttgatgcc acttggtaac ttgaccctac tctttaactg actcaaaaaa
     4021 tccctccccc ttcattacaa tatctccatt ttttaaatac ccttggaggg gtggatgaaa
     4081 ccttttattc tatggtcaac ctgctttgtc tatgctttct tataactact taagcttatc
     4141 cgctaacctt attatccttc caagcctttc cccggtcttt tccagaagaa tccccacacc
     4201 atccatggca tcaaggagcc ccattggcct gtcaacaatg ggcaggatca ggtctatacc
     4261 cattgtatga agaatctccg taccctctcc aacacctcca gccattgcaa caaggggctt
     4321 gttaagcttt cttgcccgtt tggccacccc tgcaggaacc ttgccctttg aagattgtcc
     4381 atcaatcctt ccctctcctg ttattacaag gtcaacatcc ttaagcagac catcaaactc
     4441 cagaatatca aggaccattt ctattcctgg cctaaggtca gcaccgcaaa aggccataag
     4501 accaccccca agaccacctg cagctccgga gcctggtaca tccccaatgt caacatccat
     4561 atcctcttta agtatatccg ccaaatgcct gagattccta tccagcagct ggaccatctg
     4621 ggggtcggca cccttttggg gaccgtagac ataggctgca ccacccggtc cgtagagagg
     4681 attttcaaca tcgcacataa cagttatctg ggcatcctca agaagtgggg tgacatttga
     4741 cctgtcaatc cttcttagtc catccaggcc acctcctccc tccggtatct catttccatc
     4801 ccggtccagg aacctgtagc caagagccct tgccattccc actccgccat cgtttgttgc
     4861 agaacctcct attccaacat agatctttcc atagcccctt tgcaatgccg aaaggataag
     4921 ctctcctgtg ccatatgtac ttgtaaggct tgggtttctc ctctcctcct ccacaaggac
     4981 caggcctgaa gcctcagcca tttcaatgat ggcaatctgg tcctgaatta ttccatattt
     5041 tgcctggacc ctatcgccca agggaccagt tacctcttcg catacaaact tcccgtccat
     5101 gccgtaaacc agggtttcca ctgttccctc gccaccgtca gccattggaa gcttaagtac
     5161 ttccaattct ggaatgattc ttagaagtcc cttctctatt ctttcggcaa cctccatggt
     5221 ggtggccgat cccttgaatg agtccgatgc tataagtatc ttcatttctc tgcctcctgt
     5281 gatttctcca tacccctgcc atacctgtat ttctccctta agacccctcc ataaccgctc
     5341 actccaaaga gtgcaattga aatcagaagg attcctgtga ctatcctaag gttcaagggc
     5401 tctgcaagga agaagtagcc tataagagct gccatcacca tcctgctgga ggacagcatg
     5461 gacccagttg cagaatccac atacttgaag ccctcggtgg tgaagacctg gccaagaaag
     5521 ccagttcccg cggccaggag tacaatcccg gctgaatcca ttgataagat cccgatatca
     5581 tcaatgacca ggggaaagtt gagaaacatc cctattagca taaggtaaaa cacaatgatt
     5641 atgctggact ccgtccttct ggcctgggtc aggtagagga ttgatgtccc cgcaaagaat
     5701 gcagacccaa atgatatcag gtcaccgatg tttatgctgc caaggtgtgg atcggaaaca
     5761 atgtatgatc ccacaagaat aaggaaaaga tatataaagg cacttggcct taagctttcc
     5821 cctgtaataa acctcgccag cagaaaaaca aacaccggat atgtaaggtg gatcatgttg
     5881 gcgtttgtaa tggtggtgta gttaagggat gcggtaagta gaatcaatgc ggaggcattc
     5941 agtgcagacc ttatgaaaac cggctttttc ctcactggct taaaggagat tccccggtag
     6001 accataaagg cccccagacc caccgtgccc agaaaatacc tgacaaagga agtgaccaaa
     6061 gcattcattt ccgttgtatt tgaaaccagc tttccaaaaa acgtacttaa tgtaaaaaac
     6121 agagatgaca aaagcatgag gaatactccc attacgaccc ttctttccaa tttgatactt
     6181 agattatagc atatttcgtc actcaaagga aaatcccggg aataatcccg ggaccggttt
     6241 aacctagtag gtctttgtac atttcacgtt caattccaat gtatctctcc acttcctggt
     6301 ggagcttttc catcttcctg taggttgatt cgtaggttgg acacccactc tcaggcattg
     6361 tgtaggtgtt ggttttctcc tttagtccct caagctcccc caagaggatg tcattgtccc
     6421 cctcaacctc ctgaatagtc ctttccattt cctccaaaag ctccttggac ggttccttct
     6481 ggtagtccca tatcattggc agaagcacca tctgcttctt tacaagagcc aattcaagct
     6541 cgttctggac cctgccgtac tgtctatgga cctggatcag ctcctccttg caatcatggt
     6601 aatggacctt tagtatcttg tatgcaaggc tctttattac aggcagctcc tccttcaaga
     6661 acccatgatg gttgtctgca atatccttga ttgttttctt tatatccatg tataccctcc
     6721 tgttgatttt cttctgtcat atatacccca agggatggac catatctcaa attttatcat
     6781 agaagagggt ccattccctc cagccccaat cactcatcct tcttggacat acctttccac
     6841 tggcatcgaa atgtctgacc accctttcct tggggatgcc gtgcttattc ataaggtccc
     6901 taactagtaa aatccccctc ctttttgtct cctcccagtc cccatcggaa tttacgcaaa
     6961 gctcgacccc aatggagttg ctgtttgtta tcccatgcct tcccctgccg tcaccacaat
     7021 gccaggaggc attaaagtcc tcaacaagct ggatcgtttc tccggcatcc acaaagtagt
     7081 gggctgatgc attcctgcct ggatgattga aatacctgta atgggcgtat gcatcagccc
     7141 ccttcctggg atttcctgtg tcgtgaagga caatgtactc aatttttctg cccattcttg
     7201 tagcgtaatt gtagctgatc aattcctttc tatacatgct cttcctccat ttcttttctc
     7261 atcctttcca aggcccttct tttggtattt actacggttc tgtaggtgat gccatgtcgc
     7321 tttgaaatat ctccaatgga aaggttctca agatagtagc cctggaccac ctgcctttcc
     7381 cttaagggaa gcctggagat catctggagg agctcagaac cttgctccct cctgatgtag
     7441 tcttcctcca acttaaaacc atcttccaaa aggtccatgt ggtcctctcc atcctcgcca
     7501 acctgtacat tcagggacag gattctccgg ttcctgtttt ttccaaggta gagatacttt
     7561 agccttgatt tgatgaatcc cagatatggc acactgtagt cctccctgta gtcccctatg
     7621 gactggagaa ccaggagtct tccctcctga acaaggtctt ccatatcctc ggtcttgtta
     7681 taatactttt ttattgaaga aattatcagg ggctgaagcc tcataagcag ctccaatccc
     7741 gcatcctccc tgccacttgc cacgtccata tagatttcct gaattgtcct ttccaagcct
     7801 ttctcctttc aaggcgcctt actttattcc cgggtgacat aagagtaaca ggttcagacc
     7861 aaaaacaaaa aaatagagca gccactgttc gtgtagctac tcgtatccat tttccatttt
     7921 taggagcaag gcgtcaattt tcatgtacag ccttgcaatg tccgacttca ttgcaggtcc
     7981 gtcctgactt gtgtagtatt caattgcctc ccccacaagc tgagacctct ttttttccat
     8041 cagctcccta acaaggttac cctgcctcag ctgctttttt gcggtttcca gtttgcagag
     8101 acaaggatgg tcgacaccat tttccattcg tacaaatgta actccttcac tggcgaaaat
     8161 cttcctgata ccaccaagct tgaagtatcc cattgcaccg tcatcctttc caattggctc
     8221 ccgaaccttc agctgaatgt atattgtcct ggaatcgatt ggtatgggca gattggtcct
     8281 tgcagaaatc agcttgccgt agtggttgtt ggatgcctta aggtcaaaat ggtggtagga
     8341 gcaaagcttt ctcaaaaagg tcctaagtgt actacttacc accaccatgt tctccttcaa
     8401 atataccctt gtacagtttc cggattctcc ctcgtaggaa ggaatcatca caagtatatc
     8461 ctctttcatc aattccttca tctattctac ctcctgatgc agctccgtgg cttgagcctt
     8521 ttcttttcga taccctgtta ggaacaacta agaaaaatat accagaacac atgttcgttg
     8581 tcaaggatca aaaaacctgc tcccagaagg cagcaggcca ttttcaacca ttattccaca
     8641 aagttatcca gctaattcag taattgtctg gagcatcagt tggataccat tttccagatc
     8701 atcgtactcg gtccattcct ctggatcgtg actgagtcca ccaacacttg gtacaaagat
     8761 aatgtttgca ggtgcaagag cagccatgac cattgcatcg tgggcagctc cggagttcat
     8821 gacacggtac ttcagtccaa ggtcttcagc ctttctcctt agcaggtccc cattgtccct
     8881 gtccatttca acaggtggaa tgtatacccc tctcttgaat tctatttcaa cagggtcagc
     8941 atagcctatc ttctccagct ctttttgcac cttgtcactc atggtgttga taagctggga
     9001 atccgtagcc cttatatcca cagaaagctt tacaagttcg gggataaggt tggacgcatt
     9061 tggatagatg ctcatttctc caacagttgc cactgcaccc tttcctgttt cccttgcaat
     9121 ctcgtttaca gccacaataa acttggctgc agaaacaagg gcatccttcc taccggccat
     9181 tggagttatg ccggcatggc cagcaagacc cctgaacttg acctcatacc tgtcaagacc
     9241 cacaatcgta tcaacgattc ctacatccat tccagccttt tcaaggactg ggccctgttc
     9301 aatgtgcagc tccaggaatg ctttcaaaga tcccttttcc ctttttgcca aatccaggtc
     9361 gacctttatt ccgcattcct ccatggcctc cctcaagggc ttgccgttaa tgtccttggt
     9421 cctgtcaagc tcttcctgtg taaggaagcc tgccattgcc cttgtatttg acatggcatt
     9481 gccaaacctt gccccctcct cgtcattgaa ggcggcaacc tcgattgggt gcctgtgtga
     9541 agcgccgttt tcctttaggg ttctagctat ttcaatgcct gccactattc cagccactcc
     9601 gtcgaagggg cctccgccta ttcccgtatc gtaatgggat ccaatcataa ccggtggcag
     9661 gtcaggttca gtaccttccc ttctaccaaa caggcttcca tatccatcga gataaacctc
     9721 cagaccagct gcctccatct gtgccataag gtagcccttg gcctgaacat cctccttgga
     9781 gaatgccagc cttgtaacgc ccctacccgg tgtactgctg aaggatgcca ggttgttaat
     9841 gtccctcttt actcgttcaa tatttatctt catatgaatc cacctttcca gttagattat
     9901 tcacctagaa acttgcttct ttcctctttt ggaacaaggc ttccaccggt tgaccaaacc
     9961 agctgtacag gatcacggtc aatgtatctg ccctttgtta gaaacagtcc gttaaagcct
    10021 gctgttgctg aaggttcaac aaagattccc tcctgcccat acagggcctt cagatactta
    10081 aatagcttgt catcctggat tgtgaaggct cctgtcagga gcttgtccat aagcctgccc
    10141 acaaaggagg acggccttcc aacggccaac ccgtctgcct cagtcctgtt gtcaagtcca
    10201 atgtcctcaa ccgatatccc gtcgtgcttg cctgttgcaa gtcctagaag catacagggg
    10261 gaatgggtgg gctcagcaaa atggcattca acaaggtcgc catagcttag cttgagacca
    10321 taggccactc ctccaggtcc tcctcccacc ccacagggca ggtagacaac caggggatgc
    10381 ctttcatcta tgatcacacc catttcctca agctgggatt taaccctcat tcctccaacg
    10441 gcatagccca gaaacaggtc cctggagttt tcgtcatcca caaagtggct catggggtca
    10501 ctgtcggaat ctcttcttcc ttcctctaca gccttggagt agtcatcagg gtactccaca
    10561 accttgactc ccttggaccg gagcatgtcc ttcttccact gcttggcgtc cgaggacatg
    10621 tgtaccgtca ccttaaagcc cagctttgcg ctcatgatac ctatgctcag gccaaggttt
    10681 ccagtgcttc caacctgtat cttgtatctg ctgaaaaagt caacaaactc cctttcggcc
    10741 agctttctgt aatcgtggtc atattggagc atgccatttt caaccgccag ggtttccgca
    10801 tgcttgagga cttcgtagat tccccctctt gcctttatgg acccggcaat tgagagcagg
    10861 tcatcccttt ttaagtacaa ctccccaggt atggtgctac cgctgtcccg ctcgatccag
    10921 gacatcattt ccggtatatg ggagatggga gattctataa ttccatcctt tgattcagga
    10981 aatacctttg caagataggg tgcaaaccga cggagtctct tttccgcctc cattacctct
    11041 tcttcagtaa tggggagttc atcccttatg tcatcaaatt ttctgtatcc agggttggtc
    11101 cagtatacct cccgcatgga tttgaggtca tttattatat ccataatatc cctccaaata
    11161 taatagtctc agaatatcct tctgagacta ttatacccca aatgcatttg tttgtcttta
    11221 ttgttgtagt gtgaaaaaat tgacaatggt gtctttgcaa agtcagggtc aaataatgta
    11281 gtgtcatcct gaacgaatgt gaaggatctt gcctttgcta ttctcaaaag ctttaagact
    11341 cctcgcaggc tcagagtgac aatatctttg tcatcctgaa cgaatgtgaa ggatcttgtc
    11401 tttgctattc tcaaaagctt taagactctt cgcaagctca gagtgacaat atctttgtca
    11461 tcctgaacga atgtgaagga tcttatctgt aaatgtcttc tgataaatct ctccattctg
    11521 gattaaactc actgaccaag tcatccttct tgattctttt ccaaccttta atttcctttt
    11581 ctctcttaat tgcttctctg acatcatcag tagaatcata ataaacaagc ttttccacat
    11641 tgtatttatt tgtgaagcca tcagccaatt tattcttgtg ttcatatatt ctgcgttgta
    11701 aattaccggt tactcctgtg tacaacacct tattgttcca atttgcaagg atatatacgt
    11761 aatacaaggt atcacctcta tgtagtaatt ttgtaacagc acttaagact cttcgcaggc
    11821 tcagagtgac aagtgatttg ttaattagac actgtttctc aaattaaccg gtctctcacc
    11881 ccttgcaatc cttcccacat cccttgccgt aatcctcaat tgctggtcct gggcctcgtc
    11941 tgagaagtag gcgctgtggg gggtgactat aacattctta agctccaaga gacccctttc
    12001 agcctc
//
LOCUS       DASRQF010000171         3250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_153064, whole genome shotgun sequence.
ACCESSION   DASRQF010000171 DASRQF010000000
VERSION     DASRQF010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153064"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VFD79_04740"
     CDS             <1..363
                     /locus_tag="VFD79_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HZH93388.1"
                     /translation="GLKRLGEAGFKLTVLTADTFGTVREECEGLPVNVMVFDKGSAED
                     SKRQIVQRLVSERTVAIGNGRNDMAMLGEARLGIAVIGQEGAFTGILQTADIAVTSIE
                     NAMDLFLNPKRIEATLRK"
     gene            402..1217
                     /locus_tag="VFD79_04745"
     CDS             402..1217
                     /locus_tag="VFD79_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZH93389.1"
                     /translation="MKMKTILLAIIILAMGIFVYNKNQISQFRTERIQVQSSKVKDEI
                     RIVQVTDFHSNHRIDISRLQKEIREFNPHMIALTGDLVDHNTEDLTVSLDLVDSMLSA
                     DVPVYFVEGNHEVANRHRRELLNELEEKGVIILSNNRVEMVIEDKTVNIYGADFYVEE
                     NDYQKMLEDNEAERLNILLSHSPVRASWYADGDVDLILSGHTHGGQIRLPLIGGVVSP
                     GQGLFPHYDKGLMELPGGSMLYIDSGIGNSVLPIRIMNPVQYTQIRVTGDGSF"
     gene            complement(1247..2674)
                     /locus_tag="VFD79_04750"
     CDS             complement(1247..2674)
                     /locus_tag="VFD79_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93390.1"
                     /translation="MGKKVFMFNSKYMVLIFIFTFSVLLSISSVASAATLTDHYQEVN
                     DQVSFIQANSTLADKGNFFIRRIVSKVDHTYWTSFRIPGEAGTLKYFDDHKNLVEVAM
                     VEGPLPQGRIIFLSTDSYHYILGQPYTYRDLGAGTKDSLLSHPIQLKKDQSGWVVTFR
                     YSLSPGVHGILWGVGSPQDLVDFDNKDQFRLWSNYDLDKNARLLYDGYHYKNPTTYFP
                     TSPNSYWRIPSDYLTNSMVRSGGSPASEIIGSSLLKIAHKNIGGEGFLPTLPRSNWLY
                     EDYGIEAGFFDTRFNADTIETNIIAYKKYGDPVYRDMAIRLADYYLDHGRNNRFVLQD
                     PTLGEGWLVEDYYHKSGRKNHTSLNHQLQAIHSFLLLYESEKNPEYLDFAQKMINGIK
                     ITRDMWIMEDGNLEYAYMPDGTMGLVDYDYLTYNDLLNVQEALARIKGQSDQDLQILM
                     ESKKLWMDRNNVSGYRITEPTTNPS"
     gene            complement(2819..3160)
                     /locus_tag="VFD79_04755"
     CDS             complement(2819..3160)
                     /locus_tag="VFD79_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93391.1"
                     /translation="MKKNSLILVGVALLMVIAFILVRNQMEKPSDDNFVYVATDSLGP
                     WYFDKSTIDERFDQEENISVIDVWVRHDIDQERNVVVRDELLWHIDPDQVRYKFSDAF
                     AFNEEGYLLEA"
BASE COUNT          928 a          716 c          717 g          889 t
ORIGIN      
        1 ggacttaaga gactgggaga ggcaggcttc aagcttacag tccttactgc cgacaccttt
       61 ggaacggtca gggaggagtg cgaaggcctg ccggtcaatg ttatggtttt tgacaagggc
      121 agtgctgagg atagcaagag gcagattgtc cagaggctgg tctcggaaag aaccgttgca
      181 atcggaaatg gaagaaacga tatggctatg ctgggtgaag ccagacttgg aattgcagta
      241 attggccagg agggagcatt tacaggcata ctccagactg ctgatatcgc agtgacatcc
      301 attgaaaatg ccatggacct ctttctaaat ccaaaaagaa tagaggcgac acttagaaaa
      361 taggaccata catgcaggaa gactgcggag gcgggagata catgaaaatg aaaacaatac
      421 ttctggcaat tataatcctt gcaatgggga tatttgtcta caacaaaaat cagatatccc
      481 agttcaggac tgaaaggata caggtccaat caagcaaggt gaaggatgag ataagaatag
      541 ttcaggtgac tgattttcat tcaaatcaca ggatagatat cagtaggtta cagaaagaga
      601 ttcgggaatt caatccacac atgattgcct tgacagggga cctggtggac cacaatacag
      661 aggacctcac cgtatcccta gacctggtgg attcaatgct atcagcagat gtacccgtat
      721 actttgtaga gggcaaccat gaagtagcca acagacacag aagagagctc ctaaatgagc
      781 ttgaagaaaa gggtgtcatc atcctaagca acaatagggt ggagatggtc attgaagaca
      841 aaaccgtcaa tatctatggt gcagacttct atgtggagga aaatgactac cagaaaatgc
      901 tggaggacaa tgaggctgag aggctaaaca tccttctgtc ccattctcct gtgagggcct
      961 cctggtatgc agacggggat gtggacctga ttctttccgg acatacccac gggggtcaaa
     1021 taagactacc cttgattggt ggagtggtat ctccaggcca gggtttgttt ccccactacg
     1081 acaaggggct aatggaactg ccagggggca gcatgctcta tatcgacagc gggatcggca
     1141 acagtgtcct tccaataagg attatgaatc ccgtacaata cacccagatt agagtcactg
     1201 gggacggttc tttttgactc actctaaaaa agatcccagc tgaaaatcag ctgggatttg
     1261 ttgtaggttc tgttattctg tacccggata cattgttcct atccatccac agttttttgg
     1321 attccataag aatctgaagg tcctggtcgc tttgtccctt tatccttgca agagcctcct
     1381 gaacatttag caggtcgttg taggtcaggt aatcataatc cacaagtccc atggttccgt
     1441 caggcatata ggcatactca aggttgccat cctccataat ccacatatcc ctggtgatct
     1501 tgattccatt tatcatcttt tgggcaaaat ccagatattc cgggttcttc tcagattcat
     1561 agaggagaag gaatgagtgt attgcctgca gctggtgatt aagggatgtg tgattctttc
     1621 ttcccgactt atgatagtag tcctcaacca gccatccctc tcccagggtt ggatcttgga
     1681 gaacaaacct gttgtttctt ccatggtcaa gatagtagtc tgccaatctg atagccatat
     1741 ccctgtaaac agggtctcca tattttttgt aggctattat gtttgtttca atggtatcag
     1801 cattaaacct ggtatcaaag aagcctgcct ctatgccata atcctcatat aaccagttgc
     1861 ttctaggcaa tgtgggaaga aatccttccc caccaatatt cttgtgggct atcttcagta
     1921 gtgatgatcc aattatttct gaagctggac tacctccaga ccttaccatg gaatttgtaa
     1981 gatagtcact aggtatcctc caatatgaat tcgggcttgt tggaaagtag gttgtcggat
     2041 tcttataatg gtagccatca taaagaagtc tggcattttt gtccaggtca taattggacc
     2101 agagtctaaa ctggtccttg ttgtcaaaat ccaccaaatc ctgaggtgaa cccacacccc
     2161 aaaggattcc atgtacacca ggacttaggc tgtagcggaa ggtaacgacc cagcccgatt
     2221 gatccttttt aagttgtata ggatggctta agagggaatc ctttgtcccc gctcccaggt
     2281 ccctgtaggt gtagggctga cctaggatat aatggtaact gtctgttgag aggaaaatga
     2341 ttctcccctg gggaagtggt ccctccacca tggctacctc caccagattc ttgtgatcat
     2401 caaaatactt caaggttcca gcctctcccg gaatcctaaa gctggtccag taagtgtggt
     2461 ccacctttga gactatcctt ctgataaaaa aattgccctt gtctgctaag gtgctatttg
     2521 cctgtataaa tgagacctgg tcattgacct cctggtagtg gtctgtaagg gttgctgcag
     2581 atgccactga acttatactt agcagtactg aaaatgtgaa tataaaaatc aataccatat
     2641 attttgaatt aaacataaat accttttttc ccatctagta tcctcccatt gcaaattaga
     2701 ttgtcaaata taatcaatca tgtccatcct tgtgatattc tgataccatt aaaatgattt
     2761 tttccgcaac acttcctggc tctacaggtc tccacgagac ctcctcataa ctgagatatc
     2821 aggcttcaag gaggtaaccc tcctcattga aggcaaaggc atcggaaaac ttatatctta
     2881 cttggtcagg gtctatgtgc cataggagtt cgtccctaac tacaacattc ctttcttgat
     2941 cgatatcatg cctaacccat acatcaataa cactgatatt ttcctcctgg tcaaatctct
     3001 catcaatggt agacttgtcg aagtaccagg gtcccagtga atcggtagcc acatagacaa
     3061 aattatcatc actgggtttt tccatctggt tccgaaccag aatgaaggca atgaccataa
     3121 gcaatgcgac accaaccaat attaaactat tctttttcat tataatcacc tatttagtta
     3181 tacccataaa taaccaaaaa tagccccaaa agaggttaca gttgtgttac ggatcgggtc
     3241 ttccaagtga
//
LOCUS       DASRQF010000172         2855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1292285, whole genome shotgun sequence.
ACCESSION   DASRQF010000172 DASRQF010000000
VERSION     DASRQF010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292285"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VFD79_04760"
     CDS             <1..292
                     /locus_tag="VFD79_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HZH93392.1"
                     /translation="AALFDLVKYTNSNFSEESSRKVVQYGYDTLMKLAMVLGILTKKD
                     EILDDEIQKLIEDRNEARKNKDFAKADQIRDGLLDQGIVLEDTRDGVKWKRV"
     gene            277..723
                     /locus_tag="VFD79_04765"
     CDS             277..723
                     /locus_tag="VFD79_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HZH93393.1"
                     /translation="MEKGLSVNYFREVNVDLQGEDLRMMSPLQLAYIGDAVYELLVRT
                     AIMSRDVNVNKLHKEATRYVKANAQAESVLKLEGLLTDEEKSFVRRGRNARINSSPKN
                     VDLRDYKYATGFECLMGYLYLSGKDERLIELFKQIYQSDTEGGNDK"
     gene            720..1511
                     /gene="thyX"
                     /locus_tag="VFD79_04770"
     CDS             720..1511
                     /gene="thyX"
                     /locus_tag="VFD79_04770"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019034656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HZH93394.1"
                     /translation="MKVELLRYTPEGEKLVASAAKLCYSPVGIGDIEEGQDDKKVASF
                     LKLLMDLGHESPIEHVSFTFGIEGVSRTLTHQLVRHRLASFSQQSQRYVRLAQFEYII
                     PPHIEGDPESKRIFMEAMEADQKAYDEISKRLYEKHYKRYLDEGRKEKDARQRAEKEA
                     IEDARYVFPNACETKIVVTMNARSLMNFFALRTCNRAQWEIRDLAIQMLGEVKKVYPV
                     LFSHAGPGCVNGPCPEGEMTCGRIVEVREFFKDLDERIAAEAAAE"
     gene            1525..2268
                     /gene="rlmB"
                     /locus_tag="VFD79_04775"
     CDS             1525..2268
                     /gene="rlmB"
                     /locus_tag="VFD79_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HZH93395.1"
                     /translation="MKEMYVSGRNPVMELLKSDKQVDKLYVLKGELKGSINKIMGKAK
                     DMGIVIQQVDKNKLDSMAEGNAHQGVVALVTGYEYSEVVDMLQLAKTRNESPFILVLD
                     GIEDTHNLGAIIRTAECAGVHGVVIPKRRAAMVNQTVYKSSAGAVEHMKVAQVGNITQ
                     TIEDLKKEGLWIYGADMDGEKEYFRTDLKGAIALVIGNEGKGLSRLVKEKCDVLVNIP
                     MTGKVGSLNASNAASILIYEVVRQSYEGK"
     gene            2255..2779
                     /locus_tag="VFD79_04780"
     CDS             2255..2779
                     /locus_tag="VFD79_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HZH93396.1"
                     /translation="MKGNKKPKEYLFVDGYNIINSWENLKEISKVDLEQARMTLVEIL
                     AEYKHYSGIEIFVVFDAYMVKGNNRSEEEYKGIRVIYTKENELADHYIEKALDEMGRE
                     MRVRVATSDWVEQQIVLARGGTRISARELQAEIEDEMRMVSRKRKRLNTRNDISIGRL
                     EDQVLHKLKDWKGK"
BASE COUNT          896 a          508 c          811 g          640 t
ORIGIN      
        1 tgcagccctg tttgaccttg taaaatacac aaattccaac ttttcggagg aatcgtcgag
       61 aaaggtggtt cagtatggct acgacaccct gatgaagctt gcaatggtcc ttggaatact
      121 cacaaagaag gatgagattc tcgatgatga aattcaaaag ctgatcgagg ataggaacga
      181 ggccagaaag aacaaggatt ttgcaaaggc tgaccagata agggacggtc ttttggacca
      241 gggcatcgtt cttgaggaca cgagagacgg tgtaaaatgg aaaagggtct aagtgtgaac
      301 tatttcaggg aagtgaacgt ggatttgcag ggggaagacc ttaggatgat gtctcccctt
      361 cagctggcat atattggaga tgcggtctat gagcttcttg tgagaacagc catcatgtcc
      421 agggacgtga atgtgaacaa gcttcacaag gaggccacaa ggtacgtcaa ggccaatgcc
      481 caggctgaat cggtattaaa gctagagggg cttcttactg atgaggagaa atcctttgtg
      541 agaaggggga gaaacgccag gatcaactcc tccccaaaga atgttgattt gagggactac
      601 aaatatgcca ctggctttga atgcctgatg ggctacttat atctatctgg gaaggacgaa
      661 aggttaatag agcttttcaa gcaaatatat cagtctgata cagaaggggg aaatgacaaa
      721 tgaaggttga attgttgaga tatactccag agggagagaa gttggtggcc tcagctgcaa
      781 agctatgcta ttcccctgta gggataggcg atattgagga gggacaggat gacaagaagg
      841 tagcctcatt tcttaagctc ctgatggacc ttggacatga aagtccaatc gagcatgtca
      901 gcttcacatt tgggatagag ggagtttcaa gaaccctgac ccaccagctt gtaagacaca
      961 ggcttgccag cttttcccag cagtcccaaa ggtatgtgag acttgcccag tttgaataca
     1021 tcatacctcc tcacatagag ggggaccctg agtccaagag gatctttatg gaggccatgg
     1081 aagctgacca gaaggcttat gacgaaatct caaagagatt gtatgaaaag cattacaaga
     1141 gatacttgga tgaaggcaga aaagagaagg atgcaaggca aagggctgag aaggaagcaa
     1201 ttgaggatgc aaggtatgta ttcccaaatg catgtgagac aaagatagtt gttaccatga
     1261 atgcaagaag cctgatgaac ttctttgcac ttaggacctg caacagggcc cagtgggaga
     1321 tcagagacct tgcaatccag atgcttgggg aagtaaagaa ggtttatccg gttctgttca
     1381 gccatgcagg accaggctgt gtgaacggac catgccctga gggggagatg acctgtggga
     1441 gaatagtgga ggtcagagaa tttttcaagg acctggatga gagaatagca gctgaggctg
     1501 cagcagaata gaggagaata aattatgaaa gaaatgtatg tatctggaag aaatccagtt
     1561 atggagcttc taaaatccga taagcaggtg gataagctgt atgttctcaa gggagaactg
     1621 aagggttcca taaacaagat aatgggcaag gcaaaggata tgggaatagt catacagcag
     1681 gttgataaga acaagcttga ctcaatggct gaaggaaatg cacatcaggg agttgtagcc
     1741 ctggtgacag gttatgagta ttcagaagtt gtggacatgc tccagctggc aaagaccagg
     1801 aatgagtccc cttttatcct ggtacttgat ggaatagagg atacccacaa cctgggagca
     1861 atcataagga cagcggagtg tgcaggggtc catggagtag taatacccaa gaggcgggct
     1921 gcaatggtca accagaccgt atacaagagc tctgcaggtg ctgtggagca catgaaggtg
     1981 gcacaggttg gcaacataac ccaaaccatt gaagacctta agaaggaggg tctgtggata
     2041 tacggtgctg acatggatgg ggaaaaggaa tacttcagga ctgacttaaa gggagccata
     2101 gccctggtga tcggcaatga aggaaagggt ctttcaagac ttgtaaagga aaagtgtgat
     2161 gttcttgtta atattccaat gactggtaag gttggttccc tgaatgcttc caatgctgca
     2221 tcaattctca tttatgaggt tgtgaggcag tcctatgaag ggaaataaaa aacccaagga
     2281 gtaccttttt gtggatggct acaacataat caactcctgg gagaacctca aggagatttc
     2341 aaaggtggac cttgaacagg ccaggatgac cctggttgaa atacttgctg agtacaagca
     2401 ctactcgggt attgaaatat ttgtagtgtt tgatgcatac atggttaagg gtaacaacag
     2461 gagtgaagag gaatacaagg gaataagggt catctacacc aaggaaaacg agctggctga
     2521 ccactacatt gaaaaggccc ttgatgaaat gggccgtgag atgcgggtca gggttgccac
     2581 atccgactgg gttgagcagc agattgttct tgctagaggt ggcaccagga tttctgcaag
     2641 ggagctccag gcggaaattg aggacgaaat gagaatggtc agcagaaaaa ggaagagact
     2701 aaacacaaga aacgacatat caatcggcag gctggaggat caggttcttc acaagctaaa
     2761 ggactggaaa gggaaatagt cataatcacc agggaggttt cacaatggtc ttcaagggtc
     2821 aagatttggt aaatggacga gtttatggca agatg
//
LOCUS       DASRQF010000173         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_146768, whole genome shotgun sequence.
ACCESSION   DASRQF010000173 DASRQF010000000
VERSION     DASRQF010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146768"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VFD79_04785"
     CDS             <1..661
                     /locus_tag="VFD79_04785"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HZH93397.1"
                     /translation="ENSCMPLFMVADGMGGHNAGEIASVMAIEKIREWMVENSTDLTS
                     VRRIKKSLVKSVEYANRHIYNYAREIDGCKGMGTTLTMGYVCDDKFVVGHVGDSRAYL
                     IRSNRISQLTQDHSLVQQLLVEGKISVKEAKSHPQRNVITRAVGTSGEIEVDILTVDI
                     QKGDTVILCSDGLTNMVPEDELKEMFISETDIQRACEKAIEGAKSRGGSDNITVLGIR
                     F"
     gene            676..>2192
                     /gene="pknB"
                     /locus_tag="VFD79_04790"
     CDS             676..>2192
                     /gene="pknB"
                     /locus_tag="VFD79_04790"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HZH93398.1"
                     /translation="MIGKKLGNRYEIIEQIGSGGMAYVYRAKCHLLDRFVAIKILKEE
                     FTDDEDFIRKFRRESQAAASLSHPNILNIYDVGSEEQDDKKIHYIVMEYIKGKTLKEV
                     IVDSGKLNVDQALLYASQIAEALSNAHKNHIIHRDIKPHNIMITEDNRVKVTDFGIAR
                     AVTSSTITTTSSVLGSVHYFSPEQARGGYTDEKSDIYSLGIVMYEMMTGKVPFNGDTP
                     IGVALKHVQEEIVPPADASADIPKEINDIIMKCVMKRQSDRYQSADELLGDLRAYIET
                     RAVPENRTMEETSEMTRVIPAVGSDMDSSKSQRVDRSRKKKKDKNKALIVTAAILAAF
                     ILATGIFAGYMKLRDNISATNDILMPSLIGMTQEEAETVAREEGFQINVVDSVPSSEF
                     EAGRIVLQNVDEGVRIRPGYPVSVTLSLGSDLVSVPLLVNRTLTEAEAILKEAGLRMN
                     VEYDFSDTVPLDVVMEQSIESFTDVEPNTRIDLVVSKGEEVREVVMIQLIGTQIS"
BASE COUNT          735 a          353 c          552 g          552 t
ORIGIN      
        1 tgaaaattcc tgtatgcctt tgttcatggt agcagatggt atgggcggac ataatgctgg
       61 agaaatcgcc agtgtcatgg ccattgagaa gattagggaa tggatggtgg aaaatagcac
      121 ggaccttacg tctgtcagaa gaataaaaaa atccctagtg aaaagtgttg agtatgccaa
      181 caggcatata tacaactatg caagggaaat tgatggctgc aagggtatgg gtacaacctt
      241 gaccatgggt tatgtttgtg atgataagtt tgttgtcggt catgtaggag atagcagggc
      301 ctatctaata aggtcaaata ggatatctca attgactcag gaccattcat tggttcagca
      361 actgctagtt gaaggcaaga tatctgtgaa ggaggcaaaa tctcatcccc aaaggaacgt
      421 aattacaaga gctgtaggaa caagcggtga gatagaggtt gatattctaa ctgtggatat
      481 ccaaaaaggt gatactgtga tattatgtag cgatgggctt acaaacatgg tacctgagga
      541 tgaacttaag gagatgttta taagtgagac agatatccaa agagcttgtg aaaaggcaat
      601 tgaaggagca aagtccaggg gcggcagcga taatattact gttttgggaa taagatttta
      661 actgaggtga agatgatgat aggtaaaaaa cttggaaata gatatgaaat aattgaacag
      721 atcggcagtg gtggcatggc ttatgtatac agagccaaat gtcatttgct ggatagattt
      781 gttgccatta aaattttaaa ggaagaattc acagatgatg aggatttcat aaggaaattc
      841 agaagggaat cccaagcggc agcaagtctt tcccatccca acatactgaa tatttatgat
      901 gtgggatcgg aagaacaaga tgataaaaag atccactata tagtgatgga gtacatcaag
      961 ggaaagactt taaaagaggt tatagtagat agtggcaagc taaatgtaga tcaggcccta
     1021 ctatatgctt cacaaatagc cgaagcctta tcaaatgcac acaagaatca cataatccat
     1081 agggatatca agccccacaa tatcatgata actgaggaca acagggtcaa ggtcaccgac
     1141 tttggtatcg ctagagctgt aacatcttca acaataacca ctacctcaag tgttctggga
     1201 tcagtgcact atttttcacc tgagcaggca agaggtggat atactgacga aaaatcagat
     1261 atctattctt tgggtattgt aatgtatgag atgatgacag ggaaagtacc ttttaacggt
     1321 gatactccaa ttggtgtagc acttaagcat gtacaagagg aaatagttcc tcctgcggat
     1381 gcttcagcag atatcccaaa ggaaattaat gacataatca tgaaatgtgt aatgaaaaga
     1441 caaagtgaca gatatcaaag cgctgatgaa ctgttggggg acttaagagc atacattgaa
     1501 accagagcgg tacctgaaaa caggaccatg gaagagacca gcgaaatgac tagggtgatt
     1561 ccagcggttg gttcagacat ggattcatct aaaagccaaa gagtggatag aagcaggaaa
     1621 aagaaaaagg acaagaacaa ggcattaatt gtaacagctg cgattttagc agccttcata
     1681 ttggctactg gtatttttgc tggctacatg aaacttaggg acaatatctc tgcaactaat
     1741 gatatcctga tgccatcatt gatcggaatg acacaggaag aggcggaaac cgtcgccagg
     1801 gaggaaggtt tccagataaa tgttgttgat agtgtaccaa gctctgagtt tgaggctgga
     1861 agaattgtct tgcagaatgt tgatgagggg gtcagaataa gaccaggcta tccggtttca
     1921 gtaacattga gcctaggttc tgatcttgtg tccgtgccac tccttgtaaa tcgtacactt
     1981 actgaagcgg aggcaatact caaggaagct ggattaagga tgaatgttga gtacgacttt
     2041 tcagataccg taccactaga tgttgtaatg gagcaaagca tagaatcatt tacggacgtg
     2101 gagcccaaca ccaggattga cttggtggtc agcaagggtg aggaagttag agaagtggtt
     2161 atgattcaat tgattggaac tcagataagt ca
//
LOCUS       DASRQF010000174         9446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1117943, whole genome shotgun sequence.
ACCESSION   DASRQF010000174 DASRQF010000000
VERSION     DASRQF010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 9446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9446
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117943"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..589)
                     /locus_tag="VFD79_04795"
     CDS             complement(44..589)
                     /locus_tag="VFD79_04795"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HZH93399.1"
                     /translation="MAKNNRTLMQGLVIGILFGFFLQKGGVTKYDVIVGQLRLIDFTV
                     IKIILTAIVVSMMGISYFYPKDMLPVKTKAGSVKNATIGGLLFGVGFGLLGYCPGTIA
                     GAIGNGYMDAVTGGLIGIVLGTMIYAMIYGKLKEKGVLTQDKYSEYSLYHHIKGSPFK
                     VTIPFGALIVIVLFIMEGVGL"
     gene            complement(601..1113)
                     /locus_tag="VFD79_04800"
     CDS             complement(601..1113)
                     /locus_tag="VFD79_04800"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HZH93400.1"
                     /translation="MDFLMLERWSPYLVGVLIGLLNVVSLLVSKKPLGASTSFMKLGG
                     IIYRSFDKEKAQNNEYYQKNELKLDWGMMLVFGIIIGAFVSSILSGDFNLIAVPSMWA
                     NEVSGSFLVRFFSALLGGVFLGVGARWAGGCTSGHGISGTSFLSVISWVAAIAFFIGG
                     IAVAFLIYGM"
     gene            complement(1138..2925)
                     /locus_tag="VFD79_04805"
     CDS             complement(1138..2925)
                     /locus_tag="VFD79_04805"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HZH93401.1"
                     /translation="MSSDVIIVFVILFAAIIMFSTEKIRNDVASIIIMLSLAWSGIIS
                     LNQAFAGFSSNAVVAIMGVMIIGYSIEKTGIMDTFANIIMRKAGTQENKVIAVVMAVA
                     GMISSFMQNIGAVALFLPAVKKIGNKSGISHQRLIMPLAFAGILGGTITMVASGPLII
                     LNDLLNKGGYDSFGLFSVTPIGLGVLLAGIFYFFMLSGKVLPGGEDKSQGRKDELLDI
                     YNLPKEIHEVAIEKDSELIGKTIEELDIWRIYGIHILALWESGSKTFIPWRKTTFKEG
                     QVLAIFGEDDHLNQFFNAFNLEHKHDLVVFRELADEKEAGFAEIILPPDSNLKGKTLE
                     EIALRKNYRIEPITYINTDGNRLNLMDWEMESGLKVIVFARWEDLNSLKKSKDFVVIS
                     EVKPPEESSRADKKVHAMSILGAGILLILLGVPLPLSFLSAAILMLFTKVIYKEELYE
                     AIDWKTVFLLAGLIPLGTAFEQTGAARLTAEAIINVVESWNVLFILIVIGLITSFFSL
                     FMSNVAATVLLVPLILIMGESFGIDPRGLALLVAVSASNSFVIPTHQVNAYIMGPGNY
                     KTADYLKAGGIMSAIFLLVTTLIVYFFYI"
     gene            complement(2999..4351)
                     /locus_tag="VFD79_04810"
     CDS             complement(2999..4351)
                     /locus_tag="VFD79_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZH93402.1"
                     /translation="MFFKKIETEGLAHYSYMVGDGDDIAVIDPMRDIQVYMHEARKAG
                     MRIKYILETHRNEDYVIGSMELSQKTGAQIYISDHEDLGHVYGERIKDGFEIQMGDIR
                     LKALHTPGHTLGHMSYVLYEEGKEEPYMVFTGDCMFMGDFGRTDFYGEENLEKMTGLL
                     YDSIYEKILPLGDHVLLLPAHGAGSACGDSMDERPYSSLGYEKATNEMFKLGSKEEFI
                     EKFAKMRVKPRYFERMEVLNVQGGDFVGDEVVLNVLTVEELEAIKEDVILVDARTKEG
                     YIGGHIPGSIFLSKGNLTAFLGSIYFPDDKLAFVIDGSMGEMEEIYWYCRRIGFDKMV
                     GYLPEAIEMWQEKGNEVDKLPTISARGYKEIENGDDFILLDIRDPEDIQEGDPAHNRV
                     NIQLKSLYENLDKLDKNKAIYVLCGSGNRATTAASYLKFEGFHAVVITGGVRMYRNIS
                     "
     gene            4542..5609
                     /locus_tag="VFD79_04815"
     CDS             4542..5609
                     /locus_tag="VFD79_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZH93403.1"
                     /translation="MKLSIGSTIQKLRQNKGISIDQFAHALGTTPDVVERWENNSSYP
                     DITMLSPIARLLGTSLDALLMFETEISPDDVTNIYKVCAEKFETGDYDDALLVCENYL
                     EAYPNSLFLKFRIGSLLQEYIQMAESEEQANKVLDRSISLLEAASVIDDLEVKQASLY
                     VLSSLYTMKEDYDKAIEVLNTLPKLNVNTDFMLSTIYSITGDSESAKIVEQESLFNNI
                     NNSIISLASMWGTALKDRELDFAMELATLQRKMIADYNLEGYLMGNNILMFADIYAVK
                     KDVDRTLDYIERYVDWALKNENSDLDLSKNKFFYLVENLTPGMSFEYQRQNFISNVIK
                     NQDYDLVKYTDRYKKIISRII"
     gene            complement(5653..6441)
                     /locus_tag="VFD79_04820"
     CDS             complement(5653..6441)
                     /locus_tag="VFD79_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93404.1"
                     /translation="MRRVFILTLLVATIFVVGCSDSMEKVTEKVDVDNIKDKAVEIVG
                     GRLEETEQVQVLKEYYSYIEQRMDEDKILNFLKDNVDNLDEFRVDEMLLQLENHLYAQ
                     GYDTKGVLQKLAPHVQHGSDEFKSYFRIWNNEVENETTDGESISISADEILERALQIE
                     NHIGRYPDGKTRTRLEDLYDTYMSLAIQGLGNQYIYSQEGKSEISEDILQIYEETIRE
                     NPDTRTARILEEYLDEIEKDGMKLDGENALYFFDNFERIIKNMK"
     gene            complement(6565..7983)
                     /locus_tag="VFD79_04825"
     CDS             complement(6565..7983)
                     /locus_tag="VFD79_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HZH93405.1"
                     /translation="MTYFIIGIIILVLGYITYGKVVDKHFGVDDSIETPANRLEDGID
                     FMPMSWKRIFLIQFLNIAGLGPIFGAIQGALFGPVAFIWIALGSIFAGGVHDYFSGML
                     SVRHEGKSISEIVGMYLGNGARQVMRVFSVVLLVLVGTVFMTGPAGLLANLNLFGLSS
                     LQVWVFIVLGYYFLATILPVDKIIGKLYPIFGASLLIMAAGIGTKLVIDGYQLPAFTL
                     SNLHPAGLPIWAILFTTIACGAISGFHATQSPMMARCMTKESYGRRVFYGSMIAEGVI
                     AMIWAAAGMAFFNGVGGLSEVLATGGGPAGVVYTISTSLLGPVGGVLAMLGVIACPIT
                     SGDTAFRSARLTIADAMNFDQAPIKKRLLLSLPLFVVGFALTRIDFNIIWRYFAWSNQ
                     TLAMIVLWAASAYLVKMAKSHWMTSLPATFMTAVSITYILQAPEGFSLPTTIAYPAGA
                     FAAIAAIGFFIYKTKLEAVPAK"
     gene            complement(8101..8862)
                     /locus_tag="VFD79_04830"
     CDS             complement(8101..8862)
                     /locus_tag="VFD79_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HZH93406.1"
                     /translation="MIRCIVVDDEKPAREELAWMISRRGDFQVAATAENGIEALEKSW
                     QTGAQLVFLDINMPGISGVQVAQELAKRENPPYIVFVTAYDKYAIKAFEVNAVDYLLK
                     PVSEERLEKSLEKIKRIIDQRNLLETEKIDNIIKEMGMGRPSRIVGYRNEKLIPIETR
                     EILFISIEDKTTYVHTSKGRFTINSTLSEIYDRLKGEAFFRSHKSYILNLDLIESIEP
                     WFNSTYNVRYKGIKEGIPVSRTYAREFRERMNMDL"
     gene            complement(8859..>9446)
                     /locus_tag="VFD79_04835"
     CDS             complement(8859..>9446)
                     /locus_tag="VFD79_04835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZH93407.1"
                     /translation="QINPHFLFNAINTIVSYTRTQPDEARKLLVHLGDFFRSNLQEPT
                     DKVPLKKEIEHIVSYVEIEKARHGDKLRINYEISPEAECEIPPLLLQPLVENAIKHGV
                     LHRLDGGNVTISAIGSDKGTVIEVVDDGVGIPEDVLNSLLSDCGPRDCIGLKNVHDRL
                     INLYGKDYGLNITSRENEGTRVRMVIPTGREVYAL"
BASE COUNT         2554 a         2339 c         1803 g         2750 t
ORIGIN      
        1 tgatcctccc cttccaaata gaggaggcga aaacattcgc ctcctaaagt cctacacctt
       61 ccattatgaa aagtacaatc actatcaatg ccccaaaggg tattgtcacc ttgaagggac
      121 ttccctttat gtggtggtaa agactgtact cactgtactt gtcctgtgtc agcactccct
      181 tttccttgag tttcccatat atcatggcat agatcattgt ccctaggaca atcccaatca
      241 gccctccagt gactgcatcc atgtatccgt ttcctatggc accggctatt gtgccaggac
      301 aatagcccag aagaccaaat ccaaccccaa agaggagtcc gcctattgtt gcgttcttga
      361 ctgatcctgc ctttgtcttt actggaagca tgtccttggg ataaaaatag cttattccca
      421 tcatggatac aactattgca gtaaggatga tttttattac ggtaaaatca atgagcctaa
      481 gctgtccaac tatgacatca tacttggtga cccctccctt ttgtagaaag aagccaaaaa
      541 ggattcctat tacaagaccc tgcatcaagg ttctattatt ctttgccatt ttaatcacct
      601 ctacattcca tagatcagga aggccacagc tataccgcca ataaagaatg cgattgcagc
      661 cacccagcta ataactgaaa gaaagctggt tccgcttatt ccatggccac ttgtgcagcc
      721 accagcccat cttgctccta cgccaaggaa gacccctccc agcaaggccg agaaaaacct
      781 aaccaaaaag ctccctgaaa cctcgtttgc ccacatgctt ggaactgcaa tgagattgaa
      841 gtctccagac aggattgagg atacaaatgc tccgatgata attccaaata ccagcatcat
      901 gccccagtcc agcttaagct cattcttttg ataatactcg ttgttctgag ccttttcctt
      961 gtcaaaggac ctgtagatta tccctcccag cttcataaag ctcgtggatg cgccaagggg
     1021 cttcttggaa accagaaggg aaactacatt caaaagcccg atcaataccc caacaagata
     1081 gggcgaccat ctttcaagca ttaaaaaatc catctttatc ctcctttgct aatcgtccta
     1141 tatatagaag aaatacacta tcaatgttgt cacaagaagg aagattgcac tcatgatccc
     1201 tcctgccttt aggtagtctg ctgttttata gttacccgga cccattatat aggcatttac
     1261 ctgatgggta ggtatgacaa atgaatttga tgcagacact gccacaagaa gagcaagacc
     1321 tctgggatcg attccaaagc tctcacccat gataagtatc aggggcacca gtaaaactgt
     1381 tgccgcaaca tttgacatga agagtgaaaa gaatgatgtt ataagcccga ttactatcag
     1441 gataaaaagc acattccagg attccactac atttattatg gcttcagcgg tcagccttgc
     1501 tgcaccggtt tgttcaaaag cagttcccaa gggtatcagc cccgccagca gaaagacagt
     1561 tttccagtct attgcctcgt agagctcctc cttgtagatt accttggtga acagcataag
     1621 gatagctgct gaaagaaagc ttagtggaag agggactccc aggagtatca agagtattcc
     1681 cgctccaagt atggacattg cgtggacctt cttgtcggcc cttgagcttt cctccggtgg
     1741 cttcacttca cttatgacca caaaatcctt tgacttcttc agactgttaa ggtcttccca
     1801 ccgtgcaaaa actattacct tcaaacccga ctccatttcc cagtccatga ggttaagcct
     1861 gttcccatct gtgttgatgt atgttatagg ctctattctg taattctttc ttagagctat
     1921 ctcctcaagg gtttttccct tgagatttga atcgggaggc aggattattt ccgcaaagcc
     1981 agcttccttc tcatctgcaa gctccctgaa cacaacaaga tcatgcttgt gttcaagatt
     2041 gaaggcattg aaaaattgat taaggtggtc atcctctccg aatattgcca atacctgccc
     2101 ttccttgaag gttgtctttc tccatgggat aaatgtctta ctgccggatt cccacagggc
     2161 caggatgtgg attccataga ttctccaaat atcaagctct tctatggttt tcccaattaa
     2221 ctcgctgtcc ttctcaattg caacctcgtg gatttccttg ggaagattat agatgtcaag
     2281 aagctcatcc ttccttccct gagatttatc ttctcctcct ggcaacacct tgcctgaaag
     2341 catgaaaaag taaaaaatcc ctgccaggag cacgcccagc cctatgggtg ttacgctgaa
     2401 aagcccgaag ctgtcatagc ctcccttgtt gagaaggtcg ttcaatatta tcaaaggccc
     2461 tgaagcgacc attgttatgg ttccacccag aatccctgca aaggccagtg gcattataag
     2521 cctttgatgg cttatcccag acttgtttcc gattttcttg actgcaggca agaacagtgc
     2581 aacagccccg atattctgca taaatgatga aatcatgccg gccactgcca taacaactgc
     2641 tattaccttg ttctcctggg ttcctgcctt cctcattatt atgtttgcaa atgtatccat
     2701 tatcccagtc ttttcaatac tgtaacctat gatcattact cccattattg caacgacagc
     2761 atttgacgag aatcctgcaa aggcctgatt cagggaaatt atcccactcc atgccaggga
     2821 gagcattatg ataatggatg caacgtcatt cctgattttt tctgtggaga acattatgat
     2881 tgcagcaaaa agtataacaa atacaattat tacatcacta ctcatggcaa tcctcccctg
     2941 atttagaaat caagcagcct ccatagatga gcctgcttga tcctttccct ctctatctct
     3001 atgagatgtt tctgtacatc ctcaccccac ctgttattac aactgcatgg aagccttcga
     3061 acttcagata ggatgcagct gtggtggccc tgttccccga gccgcataga acatatatgg
     3121 ccttgttctt atccagcttg tccagatttt catataggct cttcaattga atgttcaccc
     3181 tgttgtgggc tgggtcaccc tcctgaatat cctctggatc ccgtatgtcc agtaatatga
     3241 aatcatcgcc attctcaatc tccttgtagc ctctggcaga tattgtagga agcttgtcaa
     3301 cttcatttcc cttttcctgc cacatttcaa ttgcctctgg aaggtaacca accattttat
     3361 caaagccaat tctcctgcag taccagtata tttcttccat ttcccccatt gaaccatcta
     3421 ttacaaaggc aagcttgtca tccggaaagt atattgagcc aagaaatgca gtaaggtttc
     3481 ccttggacag aaatattgag cctggtatgt ggccgccaat gtagccctct tttgtcctgg
     3541 cgtcaacaag tatcacatct tccttgatgg cctccagttc ttccactgta agtacgttaa
     3601 ggacaacctc atctccaaca aagtctcctc cttggacatt gaggacctcc atcctctcaa
     3661 agtatctagg ctttactctc atttttgcaa acttctcgat gaactcctcc tttgacccca
     3721 gtttaaacat ctcattggtt gccttttcat aaccaagtga ggagtagggc ctttcatcca
     3781 ttgaatctcc acaggcagac cctgcaccgt gtgccggaag caatagaaca tggtccccaa
     3841 gtggcaaaat cttctcataa atactatcat acagtaggcc tgtcatcttt tctaggtttt
     3901 cctccccata gaagtccgtc cttccgaaat ctcccataaa catgcagtct ccagtaaaga
     3961 ccatgtatgg ctcctcttta ccctcttcgt acaaaacgta gctcatatgc cccaaggtgt
     4021 ggccaggtgt atgtaatgcc tttagcctta tgtctcccat ttggatttca aagccgtcct
     4081 tgattctctc accatagaca tgaccaaggt cctcatggtc tgatatgtat atctgagccc
     4141 ctgtcttctg ggaaagctcc attgatccaa ttacataatc ctcgtttcta tgggtttcaa
     4201 ggatatactt tattctcata ccggcttttc tcgcttcatg catgtagacc tgaatatctc
     4261 tcatcgggtc aataactgca atgtcgtccc catctcccac catataggaa taatgagcca
     4321 gtccttcagt ttcaattttc ttgaaaaaca taaaatccct ccttcttggt tccagtttcc
     4381 ttatatttct accccgtctg ttttggtcta aaacccatta ccataatctg ttaaaccggt
     4441 aactggataa attggcaatt taagttatta agatgcactc taaagggtat aattgaatat
     4501 aggtaaattc ggcaattata ttaatgtaca aggaggaaac aatgaaacta tcaataggaa
     4561 gcaccatcca gaaattgaga cagaacaagg ggatttcaat agatcagttt gcccatgcac
     4621 ttggtacgac acctgatgtt gttgaaaggt gggagaacaa ttcctcctat cctgatatta
     4681 caatgctttc acctatagcg aggctcctgg ggacatcact ggatgcgctg ttgatgtttg
     4741 aaacggagat cagtccggat gatgttacaa acatttacaa ggtgtgtgct gagaagtttg
     4801 agacagggga ctatgatgat gcccttttgg tttgtgagaa ctaccttgag gcatatccca
     4861 acagcctttt ccttaagttc aggatcggaa gccttctcca ggaatacatc cagatggctg
     4921 aatccgaaga acaggcaaac aaggtcctgg acaggtccat atcccttctg gaagctgcat
     4981 ccgtaataga tgaccttgag gtcaagcagg cttcacttta tgtacttagc agtctgtaca
     5041 ccatgaagga ggattatgac aaggcaatag aggtgctaaa tacactccca aagctcaatg
     5101 tgaacacaga ctttatgctt tcaacaatct attcaataac cggggacagt gaaagtgcca
     5161 aaattgtaga acaggaaagt ctcttcaaca atatcaacaa ctccatcatt tcccttgcaa
     5221 gcatgtgggg gactgccctt aaggacaggg agcttgactt tgcaatggag cttgcaaccc
     5281 ttcagagaaa gatgattgca gactataatc tggagggcta cctaatggga aacaatatcc
     5341 ttatgtttgc tgatatttac gcagtcaaga aggacgtgga caggaccctt gattacattg
     5401 aaagatacgt tgactgggca ctaaagaacg aaaactcaga tttggaccta tccaagaaca
     5461 agttcttcta ccttgttgaa aacctaactc caggcatgag ctttgaatac cagagacaga
     5521 acttcataag caatgtcatc aagaatcagg attatgacct ggtgaaatac acagacaggt
     5581 acaagaaaat catttcaagg ataatttaga ctacaagttc aaaaaacaat taaggatggt
     5641 cagttgacca tcctatttca tgttcttgat tattctttca aagttgtcga aaaagtaaag
     5701 ggcattctct ccatccagct tcatgccatc cttttcaatc tcatccaggt actcctcaag
     5761 gatccttgct gtcctggtgt caggattctc ccttatggtt tcttcataaa tctgaagaat
     5821 gtcttcactt atctcactct tgccttcctg ggagtagatg tactgatttc ccaacccctg
     5881 tattgcaaga ctcatatagg tgtcatacag gtcctccagt ctggtcctgg ttttcccatc
     5941 aggatatctt ccaatgtgat tttcaatctg aagcgccctt tccaggattt catcagctga
     6001 gatactgata ctctccccat ctgtggtctc attctccact tcattgttcc atattctaaa
     6061 gtatgacttg aattcatcgg atccatgctg gacatgtgga gctagctttt gaagcactcc
     6121 ctttgtgtca tacccctggg cgtaaagatg gttctcaagt tgaagcagca tctcgtcaac
     6181 cctgaactcg tcaaggttgt caacgttgtc cttcagaaag tttaggatct tatcctcatc
     6241 catcctctgt tcaatgtagc tgtagtattc cttgagaact tgaacctgtt cagtctcctc
     6301 aagacgacct ccgacaattt ccactgcctt gtccttgatg ttgtcaacat ccaccttctc
     6361 agtgaccttt tccatgctat cagagcagcc aacaacaaaa atggtggcta caagcaatgt
     6421 caatataaat acccttctca actcaatcaa ctcccctgct accattatac tattatttct
     6481 tgactaaatt gttacaagac tattacatca aaaaattcct ctggatttgt gaaaatccaa
     6541 aggaattgta aggttttatg caaactattt tgctggtaca gcttccaact ttgtcttgta
     6601 gatgaaaaat cctattgcag caattgcggc aaacgctcct gcaggatatg cgattgttgt
     6661 cggaaggctg aagccctcag gtgcctgaag tatatatgtt atgctcactg cggtcataaa
     6721 ggttgctgga aggcttgtca tccagtggct cttggccatc ttgaccaggt aggctgacgc
     6781 tgcccaaaga acaatcattg caagggtctg gtttgaccaa gcaaagtatc tccagattat
     6841 gttgaagtcg attcttgtaa gagcgaatcc aacaacaaac agtggcagtg aaagcagaag
     6901 tctcttcttg atcggtgcct ggtcgaagtt catggcatct gcaatggtaa gtcttgcgct
     6961 tctgaaagca gtgtcccctg atgttatggg acaagctatt acaccaagca ttgcaagcac
     7021 tccacccaca ggtccaagta gtgatgttga gattgtgtag acaactccag ctggtcctcc
     7081 tcctgttgcc aaaacctctg aaagtccccc aactccattg aaaaacgcca ttcctgctgc
     7141 agcccatatc attgcaatta caccttctgc aatcatggaa ccataaaata cccttcttcc
     7201 gtagctttcc ttggtcatac atctagccat cataggtgac tgggttgcgt ggaagcctga
     7261 aattgctcca caggctattg ttgtgaacag tatggcccat attggaagtc ctgcagggtg
     7321 caggtttgat agtgtgaatg caggtaactg gtagccatct attacaagct tggtacctat
     7381 tccagccgcc attattagca gagatgctcc aaatattgga tatagctttc cgataatctt
     7441 gtcaacaggt agtattgttg caaggaaata gtatccaagt acgataaata cccatacctg
     7501 aaggctgctt agaccaaaca ggttaaggtt ggccagaaga cctgcaggtc ctgtcatgaa
     7561 aaccgtaccc actagaacca atagaactac agaaaagacc ctcataacct gtctcgctcc
     7621 attgcctaga tacattccaa caatctctga gatgctcttt ccttcgtgtc taactgaaag
     7681 catacctgag aagtaatcgt ggacgccccc agcaaaaatg cttcccaggg ctatccagat
     7741 aaatgcaact ggcccgaata gggctccctg gatggctcca aagatcggtc caagtcctgc
     7801 aatgttaagg aattggataa ggaatattct tttccagctc atgggcatga agtctatgcc
     7861 gtcctctagt ctgtttgccg gagtctcaat tgaatcatct actccgaaat gcttgtctac
     7921 gacctttccg taggttatgt aaccaagtac caaaataatg ataccaataa taaagtatgt
     7981 catcataaaa cctcctgtta tgtattttca tatttaatat atatgaaaac aaggacggaa
     8041 ggcttaactt ttccctatta tgcataaaat cggacctaag ttgcagaatt tgcttccaag
     8101 ttacaggtcc atgttcattc tttccctgaa ttccctggca taggtcctgc tcactggaat
     8161 tccctccttg atccccttgt atctcacatt gtatgtagaa ttgaaccagg gctctatgga
     8221 ctcaatcagg tcaaggttta gaatatatga cttatggctc ctgaagaagg cctctccctt
     8281 aagcctatca tagatttcgg aaagggttga gttgatggtg aaccttccct ttgaagtatg
     8341 gacataggtg gtcttatcct cgattgatat gaacaggatc tcccttgtct ctatgggaat
     8401 cagcttttcg tttctgtagc caacaatccg cgatggtctt cccatcccca tttcctttat
     8461 gatattgtct atcttttccg tctccagcag attcctctga tcaattatcc tcttgatctt
     8521 ttccagactc ttctcaagcc tttcctctga aacaggcttg aggagataat ccacagcatt
     8581 tacctcaaag gcctttattg catatttatc ataggcggtt acaaatacta tgtagggggg
     8641 attttccctc tttgcaagct cctgagcaac ctggactcca ctgattccag gcatgtttat
     8701 atcaagaaaa acaagctgtg ccccggtttg ccaggacttc tcaagggctt ctatgccatt
     8761 ttcggcagtt gcagcaacct ggaagtctcc cctccgggat atcatccagg caagttcctc
     8821 cctggcaggt ttctcatcat ccaccacaat acaccttatc acagagcgta cacctcccgt
     8881 cctgttggta taaccatcct caccctggtt ccttcattct ccctacttgt tatgttaaga
     8941 ccataatcct ttccataaag gtttatcagc ctgtcatgaa cattcttaag ccctatgcag
     9001 tcgcgaggcc cacagtcgct taagagacta ttaagaacat cttcaggtat tcctaccccg
     9061 tcatcaacaa cctctattac agttcccttg tcgcttccaa ttgctgatat ggtgacattc
     9121 ccaccatcaa gcctatggag tactccgtgt ttaatggcat tttcaaccaa gggctgcagc
     9181 agcaggggag gtatttcaca ctccgcctca ggtgaaatct cataattgat cctgagcttg
     9241 tccccatgcc ttgccttttc tatttcaacg taggatacaa tatgctcaat ctcctttttc
     9301 aagggaacct tgtctgtagg ttcctggaga tttgacctga aaaagtctcc aagatgaact
     9361 aggagttttc ttgcctcatc aggctgtgtc cttgtatagg atactattgt attgattgca
     9421 ttgaagagaa aatgtgggtt tatctg
//
LOCUS       DASRQF010000175         3004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_753279, whole genome shotgun sequence.
ACCESSION   DASRQF010000175 DASRQF010000000
VERSION     DASRQF010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753279"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VFD79_04840"
     CDS             complement(<1..667)
                     /locus_tag="VFD79_04840"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HZH93408.1"
                     /translation="MSEILLNVTRGDRVENIHRGDIAVVDFKGNLIASLGDPYKRTYM
                     RSSAKPLQTLVVIESGAENTFGITRRELAVMCASHFSEDFHLDAVSSVLRKLDLGEDD
                     LLCGHSYSRNDKVTRQMLRDGKTARKLYNTCSGKHSGMLAVSRKLDLPLETYNKLQHP
                     LQQMTLDTIAQVCEMDRDDIGVGIDGCGVPVVEMPLYNMALSYAKLVHSSVFDEKRKM
                     AADL"
     gene            864..2654
                     /locus_tag="VFD79_04845"
     CDS             864..2654
                     /locus_tag="VFD79_04845"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HZH93409.1"
                     /translation="MLQQGIVFTVIIGALILFAQGSIRYDFVGLGALLVLVVTGIVPA
                     EEAFLGFGHPAVITVAAILVVSSSLLKTGVLDQLVRIMNRSGRGINVKIAALMIMTAV
                     LSSFMNNVGALALVMPIAIEVGKHHRISPSYLLMPVAFASLLGGLITEIGTPPNLIIS
                     TYRGSIEAEPFLFFDFAPVGVGLTLVGIIFISTIGWHLIPKRKGDEGQSDFFRIDEYM
                     SEVVIPKGSKYSNRTVEELLGDLNLDVNILSIIRGERSIVPPAANELLEEMDHLVIRA
                     DHSGLADLVKRTGFQFKGSKNIQDVSGKTLKDRSLVMAEIVLKDDSPAIGRTVIELNL
                     RHRFGVNLVAVSRRGISSYHRLQSFIFKPGDVLLIQCPSDSIQDVLGQLRALPLAERG
                     LRVGKGNRKSLKFITLVLFVSSILLTTLGILSVQISFAACALLMVLFHIITPREFYDA
                     IEWPIIIMLGSLLPVGDALQSTGGAETIANIMLGAMDYLSPSLMVTVLMLTTILLTNL
                     INNAAAAVLMAPIAISLAIKMGVSPDPMLMAVAVSASSAFMTPIGHQSNTLIMGPGGF
                     RFGDYWRMGLPVTIIVLLVGTPLILHFWPL"
     gene            complement(2700..>3004)
                     /locus_tag="VFD79_04850"
     CDS             complement(2700..>3004)
                     /locus_tag="VFD79_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93410.1"
                     /translation="RFFHVITPLIFMGISYLVLRNKRLKDIYKTTFSVVPTAVLLVYT
                     GIYLYNWPLAVYIVSALILAAIFFYLYRTKRHWMYYYAVSLISLALLYMQIRGIDI"
BASE COUNT          820 a          671 c          699 g          814 t
ORIGIN      
        1 caaggtctgc cgccattttc cttttttcat caaataccga tgagtgcact agcttggcat
       61 aggaaagggc catattgtaa aggggcattt ccacaaccgg tactccgcac ccgtcaatcc
      121 caaccccaat atcatcccta tccatttcac atacctgggc aatggtatcc agggtcatct
      181 gctgtagtgg atgctggagc ttgttgtagg tttcaagggg caggtccagc ttccttgata
      241 ccgcaagcat tcccgaatgt ttgcctgagc aggtgttgta tagctttctt gcagttttcc
      301 catctctcag catctgtctg gttaccttgt cgtttctgga atagctgtgt ccgcaaagga
      361 ggtcatcctc acccaggtca agcttcctaa gtacagagct taccgcatca aggtgaaagt
      421 cctcggaaaa gtgggaggca cacatgactg ccagctccct cctggttatt ccaaatgtat
      481 tctcagcacc agactcaata accacaagtg tctgtaaagg ctttgcactt gatcgcatat
      541 aggtcctctt gtaggggtct ccaaggcttg caatgagatt tcccttgaag tcaacaacgg
      601 caatgtctcc cctgtggatg ttctcaactc tgtctcctct ggttacgttc aataatattt
      661 cagacaaggc gattccccct ttgatttgtt taagttccta ataatagtat acagcaatgg
      721 cttggtattt gaaaaataaa tattcaaatg ccaggctaat tttcaatttg gagctaagca
      781 acagatgaaa atgggtataa tgtttgtgaa ttatgcaaca agaataagtt ggatttatta
      841 gaccatttgg aagaggtgat cctatgctgc agcaaggaat agtttttaca gtaataatcg
      901 gagcccttat cctttttgct caagggagca taaggtatga ctttgtagga cttggagccc
      961 tcttggttct tgtagtcaca ggtattgtcc cagcagagga ggcctttctt ggctttggac
     1021 atcctgctgt cataactgtt gcagcaatac tggtggtaag cagcagtctc cttaagactg
     1081 gagtacttga ccagcttgtg agaatcatga acaggagtgg aaggggaatc aatgtaaaga
     1141 ttgccgccct tatgataatg acggcagtgc tttcaagctt tatgaacaat gttggagcat
     1201 tggccctggt tatgcccatt gccattgagg tgggcaagca ccacaggata tcaccctcct
     1261 acctattgat gccagtggca tttgcatccc tacttggtgg gctgataaca gagattggaa
     1321 caccaccaaa cctaataatt tcaacctaca gggggagcat tgaagcagag cccttcctct
     1381 tctttgactt cgcacctgtg ggagtcggac tgacactggt tggaatcata tttatttcca
     1441 caattggctg gcacctgata cccaaaagga agggtgacga gggtcaatcg gatttcttca
     1501 ggatcgatga gtatatgtct gaggtggtca ttcccaaggg gagcaaatac tcaaacagaa
     1561 cagtggagga gctacttgga gaccttaatc tggatgtaaa tatacttagc ataataaggg
     1621 gggaaaggag cattgtcccg cctgctgcaa atgagctttt ggaggagatg gaccaccttg
     1681 tcataagggc agaccattca ggtcttgccg atctggtaaa gagaacgggt tttcagttca
     1741 aggggagcaa gaatatacaa gacgtctcgg gaaagaccct taaggatagg agcctggtca
     1801 tggctgaaat agtactaaag gacgattcgc ctgcaatagg gagaacagtt atcgaactta
     1861 acctcaggca caggtttgga gtcaacctgg ttgctgtgtc aagaagggga atctcctcct
     1921 accacaggct tcaatcattt atattcaagc caggggatgt cctccttatc caatgcccaa
     1981 gcgactccat ccaggatgta cttggtcagt tgagggcact gccgctggcc gaaagaggct
     2041 taagggtggg aaagggcaac agaaaatcat taaagtttat aaccctggta cttttcgtgt
     2101 catcaatcct cctgacaacc cttgggatcc ttagtgtcca gatatcattt gcagcctgcg
     2161 ccctgctaat ggtcctgttt catatcataa ccccaaggga attctacgat gcaatagaat
     2221 ggccaataat aataatgctt ggctctcttc ttcctgtggg ggatgccctt cagtcaactg
     2281 gtggagctga gacaatagca aatataatgc tgggggcaat ggattatctg agtccttccc
     2341 tcatggtaac cgtcctgatg ctgacgacaa tactcctgac aaacctaata aacaacgctg
     2401 ctgcagctgt tctaatggcc cccatagcca ttagtcttgc aatcaagatg ggagtatcgc
     2461 ctgaccccat gcttatggcc gttgcggttt ccgcatcctc ggcattcatg accccaattg
     2521 gtcaccagtc aaacaccctg ataatgggtc ctggaggatt caggtttggg gactactgga
     2581 ggatgggact tcccgtaact atcattgtcc tacttgtggg aacaccactg atacttcatt
     2641 tctggccatt ataaaaaatc cgaagcctag attggcttcg gattttcatt gaccacttgt
     2701 caaatatcaa ttccccttat ttgcatgtac aaaagtgcga gggatattaa ggaaacagca
     2761 taatagtaca tccagtgtcg tttggtccta tacaggtaaa agaaaatagc tgcaaggatc
     2821 agggcactta caatatatac agcaaggggc cagttgtaga ggtaaatacc tgtataaacc
     2881 aaaagcacag cagttggaac aaccgaaaaa gtagtcttgt agatgtcctt gagcctttta
     2941 tttctaagta caagataaga aatccccata aatatcagcg gagttatcac atgaaaaaat
     3001 cttg
//
LOCUS       DASRQF010000176         7086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_369144, whole genome shotgun sequence.
ACCESSION   DASRQF010000176 DASRQF010000000
VERSION     DASRQF010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 7086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7086
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369144"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VFD79_04855"
     CDS             <1..485
                     /locus_tag="VFD79_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HZH93411.1"
                     /translation="MDPFADKLGDGWQIVQSLYAMGSGGLLGVGLGQSRQKFFYIPEA
                     YNDFIFSIIGEELGLVGSMFVLLLYAMIIWRGYTVALHAKDAFGCYLATGITSLLAIQ
                     SFINIAVVTSSMPVTGITLPLISYGGTSLVIYLAGMGILLNISRVSTGDRRKTDENNS
                     "
     gene            469..1566
                     /gene="murG"
                     /locus_tag="VFD79_04860"
     CDS             469..1566
                     /gene="murG"
                     /locus_tag="VFD79_04860"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HZH93412.1"
                     /translation="MKIIVSGGGTGGHIYPALAIAEGIREKYTKVEILYVGTEGSLEE
                     ELAKKAGYSFRSIHVKGMPRKLNKKSIIALKELLLGLLDSSNVLRTFKPDLVIGTGGY
                     VCGPILYKAARRRIPTVIHEQNAFPGITNKILSRFVDKVLITYEGSRKYFHKSSQVVL
                     TGNPVRKEIINQDSQHARMELKLDEKPTVLSFGGSGGQKSLNNSILGIVETVSRENSI
                     NLIHVTGKPHYDDFLKKLREGGISPGDNVEILPYHHRMPLAIHAADLVITSGGAIALA
                     EISTAGKPSILVPKGYTAENHQEYNARAFQESGAAWMILEKDLTPDLLYTRIMELIKD
                     GERLLEMGENSRKLGNPAATETIIGEIAKLI"
     gene            1582..2334
                     /locus_tag="VFD79_04865"
     CDS             1582..2334
                     /locus_tag="VFD79_04865"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HZH93413.1"
                     /translation="MVKETRVQRKKRRLRLYRRIVLLIVFVGLLITWSLRTSLLDIKD
                     ITVEGNSRLSAQEIIDASGLNLGENIIKSSIGKGEEAVVGLPYIETADIGRKLPKTVK
                     IQVEERIPYMQFPYNYSYAVVDSHGVLLEYSIRRANDIPLVSGLQWEEIKLGEDLTEG
                     PMGDSLGDFFKDQEIANIVMNFRGIEYEDENNIKFTLFNGVTVEFGPLHNVKYKLKVL
                     QEIIDDVEEKNIPTKMIIMNKGEHPIVVRDEK"
     gene            2345..3073
                     /locus_tag="VFD79_04870"
     CDS             2345..3073
                     /locus_tag="VFD79_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HZH93414.1"
                     /translation="MKIKGEIVALVLVSLILGLILSIQFKTINQSVGEGVMPTQRAQE
                     LANDLRKAQSEMESQLRHIQELESKIEQYEKGGIENDIYAENLYKDAMKYRMLAGYTD
                     LVGEGIVLEINDPPVDIQFGEGFSIVDELDLILQVLSVLNAAEAEAISINDQRYTGFT
                     EIVRAGNHIEINGVSTGSPIIIKAIGNPDTLESALSIKWGIVWQLRNYDYIVHLTQDR
                     EVKVPRYRRVKDFIYATPVEETVN"
     gene            3084..3806
                     /locus_tag="VFD79_04875"
     CDS             3084..3806
                     /locus_tag="VFD79_04875"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HZH93415.1"
                     /translation="MNKNNPKFLITIFSVLVGILIASQMKLNVDLLTPVTVKSIIDTR
                     KEIDAVRNETAELEKIIAIREAELATLESINTGEANIIDILEDDVKNNRNKSGYTALQ
                     GPGVEIIMYDNIGDGIGLNFNDYIIHDLDILNIINDLRVAGAEAISINDQRIISSSEI
                     KCGGPIIRVNGKSIGTPFVINAIGDPQVLMAAVNAPGTYGNNLTKIYGIGIQPYVKDR
                     VEVAAYKGQFQFNYAKPMEEGE"
     gene            3807..4160
                     /locus_tag="VFD79_04880"
     CDS             3807..4160
                     /locus_tag="VFD79_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HZH93416.1"
                     /translation="MVYALIGILVGAIIGFLLPYTYNTAYTLYISVAILACLDSVFGG
                     IRANLENKFNTRIFISGFFGNAILAAFLAYIGDRLGFPLYYAAIFTFGSRLFDNFAKI
                     RRLWLFKEKPAETEK"
     gene            4189..5304
                     /gene="ftsZ"
                     /locus_tag="VFD79_04885"
     CDS             4189..5304
                     /gene="ftsZ"
                     /locus_tag="VFD79_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004825376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HZH93417.1"
                     /translation="MFDFDIDVDQFAKIKVFGVGGGGNNAVNRMIECGVRGIEFIALN
                     TDKQALYSSRAEQKVQLGEKITKGLGAGANPEIGSKAAEENRNEILETLKGADMVFIT
                     AGMGGGTGTGAAPVVAEIAKELGILTVGVVTKPFTFEGKKRLLHAEQGIQELKERVDT
                     LVTIPNDRLLQIADKKTTMADAFMMADEVLKQGIQGISDLISVPNLINLDFADVKTIM
                     FDKGIAHMGIGRASGDNRATEAAKQAITSPLLETSIEGARSVLLNITGGNDLGIFEVN
                     EAADIIRQSVDPDANIIFGAGIDESMSDEIQITVIATGFEEGVVKNKSTINQKAGLFG
                     TRKTEEAVEVKETQEEKGANMEDLDIPIFLRRRDRNK"
     gene            5359..5817
                     /gene="nrdR"
                     /locus_tag="VFD79_04890"
     CDS             5359..5817
                     /gene="nrdR"
                     /locus_tag="VFD79_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HZH93418.1"
                     /translation="MKCPYCEYQESKVVDSRPTDEGQAIRRRRECISCGKRFTTYEKI
                     EELPIVVVKKDGNRQVYDRNKLMNGIIKACEKRPVSMSSIEQIVDDIEKTIFNSLENE
                     ITSTQIGELVMNKLKDIDEVAYVRFASVYRQFKDVNSFMDELKKILGENK"
     gene            5831..>7086
                     /locus_tag="VFD79_04895"
     CDS             5831..>7086
                     /locus_tag="VFD79_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZH93419.1"
                     /translation="MNKNKVNELLVQNILQYGNIGVHVIDKDRKTIIYNEAMADLEGL
                     EIYQVIDKDILDIFPSLNENTSTLIKVIHSGEPILNATQTYFNYRGHSITTISSTMPL
                     YYGDEIVGAMEIAQDVTNLRKLSDQLLELQQELKETRRDSKKDPQIKKYTFGNIIGNN
                     KSLVMAKEVGERASETPSSVLIHGETGTGKEMFAQSIHNHGIRRNNTFIAQNCAAIPD
                     SLLEGILFGTEKGGFTGAIEREGIFEQANGGTLLLDEINSMSLPLQAKLLRVLQEGYI
                     RRIGGLRDIPIDVRIIATTNEDPKLGIETGSIRRDLYYRLSVINIHIPPLRERIDDLD
                     LLTRYFIRKYNKLLNKSIDKVSDQVMNDFMNYDWPGNIRELENFIEGAMNMVSSRDKV
                     LNRENFITSSFIPRSSDFGNNLISSLS"
BASE COUNT         2328 a         1331 c         1764 g         1663 t
ORIGIN      
        1 ttatggaccc atttgcagac aagctgggag acggctggca gattgtacaa tccctttatg
       61 caatgggatc gggaggactt cttggagtag gtctgggaca gagcaggcag aaattcttct
      121 atatacccga ggcctacaat gattttattt tttcgataat tggagaggag cttggactcg
      181 tggggtctat gtttgtgctc ctcctctatg ctatgataat atggagagga tatacggtgg
      241 ccctccatgc aaaggatgcc tttggctgct atcttgccac aggaatcaca tccctattgg
      301 cgattcagtc atttataaat atagccgtag taacatccag catgcctgta acgggaataa
      361 cccttcctct cataagctat ggaggtactt ccctggtcat ttatcttgct ggaatgggaa
      421 tactcttgaa catctcaagg gtatccacag gcgataggag aaaaacagat gaaaataata
      481 gttagcggag gcggaacagg gggacacatt tatcctgctc tggccattgc ggaaggcata
      541 agggaaaaat acaccaaggt ggagatactt tatgtgggca ctgaaggaag tctcgaagag
      601 gagcttgcaa aaaaggccgg ctacagcttc aggtcaatcc atgtcaaggg tatgcccaga
      661 aaattgaata agaaatcaat aattgcccta aaggagctgc tacttggtct ccttgattcc
      721 agcaatgttt taaggacctt caagccagac cttgtaattg gaaccggagg ctatgtttgc
      781 ggaccaatcc tctacaaggc tgcacgaaga aggatcccta cggtaatcca cgagcagaat
      841 gcatttcccg gcataacaaa caagatactt tcaagatttg tggacaaggt cctcataaca
      901 tacgagggtt ccaggaagta cttccacaag tcaagccagg ttgtccttac tgggaatcct
      961 gtgagaaagg aaatcatcaa ccaggacagt cagcatgccc ggatggagtt aaagcttgat
     1021 gaaaagccga cggttctttc cttcgggggt agtggaggac agaaaagtct aaacaattcc
     1081 attctgggga tagtggaaac tgtatccaga gagaattcca taaacctaat ccatgttacc
     1141 ggtaagcccc attacgatga ttttttgaaa aagctcaggg aaggaggaat ttcaccggga
     1201 gacaacgtag agattcttcc ctaccatcat agaatgccac ttgcaataca tgcagctgac
     1261 cttgtcataa ccagtggagg tgccatagcc ctggcagaga tttccactgc cggcaagcca
     1321 tccatattgg taccaaaggg atatacggct gaaaatcacc aggaatacaa tgcaagggcc
     1381 ttccaggaaa gtggagctgc atggatgatc ctggaaaagg acctgacacc tgaccttctt
     1441 tatacaagga tcatggagct gatcaaggat ggtgaaagac ttttggaaat gggagaaaac
     1501 agcagaaagc tgggaaatcc agctgcaacg gagaccataa ttggagaaat tgccaagtta
     1561 atatagatag aaggtggaac tatggtcaag gagaccaggg tgcagagaaa aaaaagaaga
     1621 ctgcgattgt acagaagaat agttcttttg attgtttttg taggtctgct gataacttgg
     1681 agcctaagga caagtctgct ggatatcaag gatattacag ttgaagggaa ttcaaggctt
     1741 tctgcccagg aaattattga cgcatcaggc ttaaatctgg gtgagaacat aatcaagtca
     1801 agcatcggga agggcgagga ggcagtcgtg ggcctaccct acattgaaac tgcagatatt
     1861 gggagaaagc ttccaaaaac agtcaagata caagttgagg agagaatccc atacatgcaa
     1921 tttccttaca attacagcta tgccgtggtt gactcacatg gagtcctcct ggaatattcc
     1981 ataagaaggg ccaatgacat ccctcttgta agcgggcttc aatgggaaga gatcaagctg
     2041 ggggaagact tgacagaagg ccccatgggt gatagcctag gggatttttt caaggaccag
     2101 gagattgcaa atatagtcat gaacttcagg ggaattgaat atgaggatga gaataatatt
     2161 aagtttactt tatttaatgg agtgacagtt gaatttggac cccttcataa tgtaaaatat
     2221 aagttaaagg tacttcagga aattatcgac gatgtagagg aaaaaaacat cccgacaaaa
     2281 atgatcatta tgaacaaggg agaacatcca atagtagtta gggatgagaa gtgaggggga
     2341 taaaatgaaa atcaagggag aaatagtagc gcttgtattg gtttccctta tccttggatt
     2401 gattttatcc atccagttca agaccataaa ccagtctgtt ggggaaggag tcatgcctac
     2461 acaaagggca caggaacttg ccaacgacct taggaaagcc caatctgaaa tggagtcgca
     2521 actaaggcac atacaggagc ttgaatcaaa gatagagcaa tatgaaaagg ggggcataga
     2581 gaatgacata tatgccgaga acctatacaa ggatgcaatg aagtatagaa tgctggcagg
     2641 ctacactgac cttgtgggag agggcattgt ccttgagata aatgatcctc ccgtggacat
     2701 tcagtttggt gaaggcttca gcatagttga cgagcttgac ctgatcctgc aggtattgag
     2761 cgtactgaat gctgcagaag cagaagcaat aagcataaat gaccagcgat acaccggctt
     2821 cactgaaata gtaagagcgg gtaaccacat agaaataaat ggagtgtcaa ccggatctcc
     2881 aattatcata aaggccatag gcaatcctga tacactggaa tctgcattga gcattaaatg
     2941 gggaatagtg tggcagctta gaaactacga ctatattgtt caccttaccc aggataggga
     3001 agttaaggtt ccaaggtata gaagggtaaa agatttcata tacgccactc ctgttgaaga
     3061 aactgtaaac taggagggtt attatgaata agaataatcc caagtttcta ataaccatat
     3121 tcagtgtcct tgtgggaatt ttaatcgctt cccagatgaa attgaatgta gacctattga
     3181 ctcctgtgac agttaagtca ataatagata caaggaagga aattgatgca gtcaggaatg
     3241 agacagctga actggaaaag atcatagcaa taagggaagc ggaattggca acccttgaaa
     3301 gcataaacac cggtgaggcc aacataattg acatcctgga agacgatgtg aaaaacaaca
     3361 ggaacaagtc gggctacaca gccttgcagg ggccaggggt agaaataata atgtatgaca
     3421 acatagggga tggcatcggc ttgaacttca atgattacat catacacgac ttggatatcc
     3481 tgaacatcat aaacgacctt agggttgcgg gagctgaggc cattagcata aatgaccaga
     3541 ggataatctc ctcaagcgaa atcaaatgtg gaggacccat tatacgtgtg aacggcaaga
     3601 gtattggaac cccttttgtg ataaatgcaa taggtgaccc ccaagtgctg atggcagctg
     3661 taaatgcgcc aggtacatac ggaaacaatc taacaaagat atatgggatt ggtatccagc
     3721 cctacgtaaa ggatagggtg gaagtggcgg cctacaaggg acagtttcaa ttcaattatg
     3781 caaaacctat ggaagaaggt gagtagatgg tatatgcttt aatagggata ctggtgggag
     3841 cgatcatagg tttccttctt ccatatacct acaacacagc ctatacactt tatatatcag
     3901 tggccatact tgcctgtctt gattcagtat tcgggggcat aagggcaaac ttggagaaca
     3961 agttcaatac caggattttt atttccggct ttttcggaaa tgcaatattg gcggcatttt
     4021 tggcatacat tggggatagg ttaggatttc cactgtatta tgcggccatt ttcacctttg
     4081 gaagcagact ttttgataac ttcgctaaaa ttagaagact ctggctattc aaggaaaagc
     4141 cggctgaaac agaaaagtaa cacccaaatg agaataggag gaaagggaat gtttgatttt
     4201 gacattgatg ttgaccaatt tgcaaaaatt aaggtttttg gagtaggtgg aggaggtaac
     4261 aatgcagtaa atcgcatgat tgaatgcgga gtgagaggaa ttgagtttat agctcttaat
     4321 acggataaac aggcattata ttcatccagg gcagagcaga aggtccagct tggggaaaag
     4381 atcaccaaag gtcttggagc aggagcaaac ccagaaattg gatccaaggc tgcagaagag
     4441 aacagaaatg aaatactgga aaccctaaag ggcgcagaca tggtcttcat cacagccggc
     4501 atggggggag gaactggaac aggggcagct cccgtggttg ctgaaatagc caaggagctt
     4561 ggaatactta ctgttggcgt ggttacaaaa ccatttacct ttgaaggtaa aaagagactg
     4621 ctacatgctg agcagggaat tcaggaacta aaggaaaggg tggacaccct tgtaaccatt
     4681 ccaaatgaca ggcttcttca gattgctgac aagaagacca ccatggccga tgccttcatg
     4741 atggctgatg aggtccttaa gcagggtata cagggaatat ctgacctgat aagtgtacca
     4801 aatcttatta accttgactt tgcagatgtt aagaccatca tgtttgacaa gggcattgca
     4861 catatgggaa ttggaagggc atcaggagac aacagggcaa ctgaggcggc aaagcaggca
     4921 attacaagtc ccttgctgga aacatcaatt gaaggtgcca gatcggttct tctaaacatc
     4981 actggaggaa atgacctggg aatatttgaa gtgaacgaag ctgctgatat cattagacag
     5041 tcagtggatc cggatgcaaa catcatattt ggagcaggaa tcgatgaaag catgtcagat
     5101 gagattcaga tcactgtaat agctacaggc tttgaagaag gcgttgtaaa aaataaatcc
     5161 acaataaacc agaaggcagg tctttttgga accagaaaaa ccgaagaagc tgttgaagtt
     5221 aaggaaacac aggaagaaaa gggagccaac atggaagatt tggacatacc aatattcctg
     5281 aggagaagag acagaaacaa gtagaccatt agacgtatcc attaaaggat acgtctttta
     5341 ctaaggaggt tggtatccgt gaagtgtcca tattgtgaat atcaggaatc caaggttgtg
     5401 gactccagac ctacagatga aggccaggca ataaggcgta gaagggagtg cataagctgc
     5461 ggcaagagat ttacaaccta tgagaagatt gaggagctac ccatagttgt agttaagaag
     5521 gatggcaaca ggcaggttta cgacagaaac aagctgatga atggaattat aaaggcctgt
     5581 gagaaacgcc cggtttccat gagctcaatc gagcagattg tggacgatat tgaaaagacc
     5641 atattcaatt cccttgagaa tgaaatcacc tcaacccaga tcggggagct ggtaatgaac
     5701 aagcttaagg acattgatga ggttgcctat gtcaggtttg catcggtcta cagacagttt
     5761 aaggatgtaa actcctttat ggatgagcta aaaaagattc taggagagaa taaatagggg
     5821 ctgttaattg atgaataaga acaaagtcaa tgaactattg gtgcaaaata ttttgcagta
     5881 cggaaatatt ggagtccacg taattgacaa ggacagaaaa acaatcatat acaacgaagc
     5941 catggcagat cttgaaggcc tggaaatcta ccaggttatt gacaaggata tcctggacat
     6001 atttccaagc cttaatgaga atacatccac ccttatcaag gtgatccatt caggtgaacc
     6061 tatcctaaac gccacccaga cctacttcaa ctacagaggt cattcaatta caaccattag
     6121 ctcaaccatg ccactatatt acggggatga aatagtggga gcaatggaaa tcgcacagga
     6181 tgttacaaat ctaagaaagc tatcagacca gctgctggag ctgcagcagg agcttaagga
     6241 gaccaggcgg gattcaaaga aggatcccca gataaagaag tacaccttcg ggaacataat
     6301 cggtaacaac aagtcgctgg taatggccaa ggaggtggga gaaagggcta gtgaaactcc
     6361 ttccagcgtc cttattcacg gtgagacagg gacgggaaag gaaatgtttg cacagtcaat
     6421 ccataaccat ggtatcagga ggaacaatac ctttatcgca cagaactgtg cagccatccc
     6481 agattcacta ctggagggaa tcctttttgg aacagaaaag ggaggcttta caggagccat
     6541 tgagagggag ggtatttttg aacaggctaa tggtggaacc ctgctattgg acgagatcaa
     6601 ctccatgagt ctacccttgc aggcaaagct ccttagggtc cttcaggagg gctatataag
     6661 gaggatcggt ggactaaggg atatacccat tgatgtaagg ataattgcaa ccaccaacga
     6721 agaccccaaa cttggaatcg agacaggctc aataagaagg gacctctact ataggcttag
     6781 tgtgataaat atacacatac cgcccttgag ggaaagaata gatgaccttg acctacttac
     6841 aaggtacttc ataaggaaat acaataaact cctaaacaag tcaatagaca aggtttcaga
     6901 ccaggtgatg aatgacttta tgaactatga ctggccggga aacatacgtg aactggaaaa
     6961 cttcattgaa ggtgcaatga atatggtttc ctccagggac aaggtcctaa acagggaaaa
     7021 tttcatcact tcaagcttta ttccaagaag ctcggatttt ggcaacaacc ttatcagtag
     7081 cctgtc
//
LOCUS       DASRQF010000177         3785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_358732, whole genome shotgun sequence.
ACCESSION   DASRQF010000177 DASRQF010000000
VERSION     DASRQF010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358732"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VFD79_04900"
     CDS             complement(<1..264)
                     /locus_tag="VFD79_04900"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZH93420.1"
                     /translation="MKFCWTTLTVNDMDKSIEFYSEIVGLKVDRRFNAGPDTEICFLG
                     EGETKLELICDTDIRPDCLGEGISMGFEVESLDLLKKELDQRDI"
     gene            complement(391..1512)
                     /locus_tag="VFD79_04905"
     CDS             complement(391..1512)
                     /locus_tag="VFD79_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93421.1"
                     /translation="MKKLLMLLLVFALSFSMLAGCTPAEEPAEEPAEEPAVEEPAEEP
                     AEEPAEEPAEAGKIAKLGLGQNISTAKSKAKADDANAQAQADVTIAAVGFDADGKVVS
                     VTVDVAQTRVAFDENMAVTSDKEELVKSKKELGPDYGMVKASGIGKEWFEQMEVFEEW
                     MIGKTVDEITGLEVMERDESHKNVPNVPELTSSVTITVESYLAAVEEAWNNAVDATGA
                     ETVGLGIETHINKSKDATADAGAQAQVDTYMAATAFDAEGNVVATIIDNAQVRVAYEL
                     DGTLATDTSVEGKTKHELKEDYGMLKASSIGKEWYEQMEAFQDWMAGKSMDDIMNLEV
                     MERDESHQHVPADADLTSSVTITVEGYQAVVQEAYEMAK"
     gene            complement(1832..2248)
                     /locus_tag="VFD79_04910"
     CDS             complement(1832..2248)
                     /locus_tag="VFD79_04910"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HZH93422.1"
                     /translation="MEHRIEPGLIKDYRKITGPSDGTDLPGSLELSYLVSTPAVVNMV
                     IEASTEMLEDLLPSDYTTVGTHIELSHEKPTLVGEPINIRLKVDEVENNKIFIVIEGF
                     DSQGKFLKGRYERHIVNKSRLMQQAYGRFPDSTSGY"
     gene            complement(2324..3043)
                     /locus_tag="VFD79_04915"
     CDS             complement(2324..3043)
                     /locus_tag="VFD79_04915"
                     /inference="COORDINATES: protein motif:HMM:NF019559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HZH93423.1"
                     /translation="MGEYGRRLIIVFLVLTVLLLLMESYGIDGNLLTFARYLVVLLLS
                     MTAYLKRGPNHEQKILFLAFPLMLIGDFFLVVSYTIGGFPENFRYAGFIPFTIAYLII
                     TRIYAKGFKWNPWLLLPTVIYGLLLMVVGFLIFPHLTGLQRTLGSMVALALTSMAWTG
                     ASTVWSGYYSRKTSIMMGLSGFLMLICDYGVAFDLFYPEVSSIRIALPLNIVWLAYIP
                     GWTLLALVVAQGDIFATVYEQ"
     gene            complement(3056..>3785)
                     /locus_tag="VFD79_04920"
     CDS             complement(3056..>3785)
                     /locus_tag="VFD79_04920"
                     /inference="COORDINATES: protein motif:HMM:NF022805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3159 domain-containing protein"
                     /protein_id="HZH93424.1"
                     /translation="KAREIERASLWDGEVRKSDMIKIMDEIVDEVRSVIGGKTIGALV
                     PPLVFVIVNNIFGIVPGVLAAALTALILGVQRYLRGETIKYALGGLAGVLLASAFAYY
                     AGNAANYFLPRIISSGALFLVSVLSILVGMPIAAFASHLSRGWELDWIMRKDIKPAYR
                     EVSIVWAMLFLLRMYLQIQLYRGGDLFLIAWANTLLGFPATLTVLVLSYIYGTWRLRN
                     LKGPGIEEFREGKPGPWEGQKKGF"
BASE COUNT         1037 a          947 c          610 g         1191 t
ORIGIN      
        1 aatatccctt tggtccagct ccttcttaag tagatccaga gattccacct caaagcccat
       61 tgatattccc tctcccaggc aatctggtct gatgtccgta tcacatatta actcaagctt
      121 agtctctcct tcacctaaaa aacagatttc cgtatccggt cctgcattaa atcttctgtc
      181 aaccttaagg cccacaattt cagagtaaaa ttcaattgat ttgtccatat cattgacagt
      241 caatgtagtc cagcaaaatt tcattacgta tcctccccta ttcatcagat aaattgtctt
      301 ccaaaccttt gatacccttg atattctttt tatcacatta cagacaaaaa acagagcctg
      361 gtttcccaga ctccgtgatt tcagatttaa ttatttagcc atctcgtaag cttcttgaac
      421 aactgcctga tatccttcaa ctgttattgt tactgatgaa gtaaggtcag catcagctgg
      481 tacatgctgg tgagattcgt ctctttccat aacctcaaga ttcattatgt cgtccattga
      541 ctttccagcc atccaatctt ggaaagcttc catttgctca taccactctt tgcctattga
      601 tgaagctttt aacattccgt agtcttcctt aagctcgtgc tttgtttttc cttctactga
      661 agtatcagtt gcaagggttc cgtcaagctc atatgctact ctaacctgtg cattgtcgat
      721 tattgtagca actacattac cttcagcatc aaaggcagtt gcagccatgt atgtgtcaac
      781 ctgtgcctgt gctcctgcat ctgcagtagc atcctttgac ttgtttatat gtgtttcaat
      841 acccaatcca acagtctctg ctccagttgc atcaacagca ttgttccatg cctcttcaac
      901 tgctgccaaa tagctttcta ctgttatggt aactgatgat gtaagctctg gtacgttagg
      961 aacattcttg tgtgactcat ctctctccat aacttcaaga cctgtaatct cgtcaacagt
     1021 cttacctatc atccactctt caaaaacttc catctgctcg aaccactctt tgccgatacc
     1081 tgaagccttt accattccat agtcaggacc aagttccttc ttgctcttaa caagctcttc
     1141 cttgtctgat gtaactgcca tgttttcgtc aaaagccact cttgtttgag caacgtcaac
     1201 tgtaacactt acaaccttgc catctgcatc aaatccaact gcagcgatag ttacatctgc
     1261 ctgtgcctgt gcatttgcat cgtcagcttt tgcctttgac ttagcagttg aaatgttttg
     1321 tccaagtcca agtttggcaa ttttcccagc ttctgctggc tcttctgctg gttcctcagc
     1381 tggctcttct gctggttctt ctactgctgg ctcttctgct ggttcctcag ctggttcctc
     1441 agctggcgta catccggcta gcatactgaa gcttagcgca aatacaagta gtagcattaa
     1501 tagtttcttc attcttaaac cctccatttt atgtattgtt tgatatatat ttaacatcta
     1561 cgattataac agataggttc ctcttccaca agtctttttt catttaaaca tttgtatatt
     1621 tgtatgtttg tgttaattat cgactaaagt cccttttttt cttcactggt ttgctaggaa
     1681 aagatacttg ttgtttatat attgaaggtt ttctatatta aagcgaccat attattttga
     1741 taaaatgaat tcaattgtct actcttacaa atacaccaat taaaataagc cagaatattt
     1801 gttagacaaa tatcctggct cctttagctt gtcaataccc acttgtcgaa tctggaaatc
     1861 taccgtaggc ttgctgcatc agtctgcttt tgtttacaat atgcctttca tatcttccct
     1921 taagaaactt accttgactg tcaaatcctt caatcacgat aaaaatcttg ttgttttcaa
     1981 cctcatccac ctttaacctg atattgatag gctcccctac cagggtaggc ttttcatgtg
     2041 acaactctat gtgtgttccc accgttgtgt aatctgaggg gagaagatcc tctagcatct
     2101 ctgtagatgc ctcaatgacc atgttcacaa ctgcaggagt tgaaacaagg tatgacagtt
     2161 ccagtgaccc tggaaggtct gtaccatctg aaggtcctgt gatcttccta tagtccttga
     2221 ttaagcctgg ttcaattcta tgttccattg atactccccc ttttataccc taagtaaata
     2281 aaacatttgg ctacctttcc tcttacttat tatatacctg tttttactgt tcataaacag
     2341 tggcaaagat atctccctga gccaccacca aggccagaag agtccaccct ggtatatatg
     2401 ccaaccaaac tatgttaaga ggcaaggcaa tccttatgct cgagacttca ggataaaaca
     2461 gatcaaatgc cactccataa tcgcagatta gcatcaagaa ccctgaaagt cccatcatta
     2521 ttgatgtttt tctactgtag tacccactcc agactgtgga agctcctgtc catgccattg
     2581 atgtaagggc cagtgcaacc attgatccca aggtcctctg taaaccagtt agatgtggga
     2641 atattaaaaa tccaaccacc ataaggagca atccataaat tactgtaggt aagagcaacc
     2701 atggattcca cttaaatccc tttgcataaa ttcttgtaat tatcaagtac gctattgtaa
     2761 atggtataaa gcctgcatat ctaaagttct cgggaaatcc acctatggta taggatacga
     2821 ccagaaagaa gtctccaatc aacattaaag gaaaagccag gaatagtatt ttctgctcgt
     2881 ggttaggccc tctcttaagg tatgcagtca tgcttaggag taataccacc aggtatcggg
     2941 caaaggtcaa aagattgcca tctattccat atgactccat tagcaaaaga agcactgtaa
     3001 ggaccaaaaa cacaattatg agccttcttc catattcacc cataccctca cctcatcaaa
     3061 aacctttttt ctgaccttcc catggacctg gctttccttc cctgaattct tcaatcccag
     3121 gtcccttcaa atttctcaat ctccaggtgc catatatata gcttagcaca agaactgtaa
     3181 gtgttgcggg aaagcccagc aaggtatttg cccatgcaat aagaaaaagg tctccccctc
     3241 tatagagctg gatctgaagg tacatcctca aaagaaaaag catggcccac acaattgata
     3301 cttccctgta tgccggcttg atgtccttcc tcataatcca atccagttcc catcctctgg
     3361 aaagatgact ggcaaaggct gcaattggca ttccaaccaa tatggatagg actgagacaa
     3421 gaaacaatgc tccacttgat ataatcctgg gcagaaaata atttgcagca ttgcctgcat
     3481 aatatgcaaa ggccgaagcc agaagaacac ctgcaagtcc ccccaaagca tactttattg
     3541 tttcccccct aaggtatctc tgtactccta aaataagggc agtcagggca gctgccaata
     3601 cccccggcac aataccaaaa atattattga ctatcacaaa aaccaatggc ggcaccaagg
     3661 ctccaatagt ttttcctcca attacactcc ttacctcatc tactatttca tccatgattt
     3721 ttatcatatc agacttcctc acttcgccat cccataaaga agctctttca atctcccttg
     3781 ctttt
//
LOCUS       DASRQF010000178         2715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1103177, whole genome shotgun sequence.
ACCESSION   DASRQF010000178 DASRQF010000000
VERSION     DASRQF010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103177"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1667
                     /locus_tag="VFD79_04925"
     CDS             <1..1667
                     /locus_tag="VFD79_04925"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HZH93425.1"
                     /translation="HEGIGYVEAVKAYEDITGDVDTFYVKPKEGLEDIVGKEFSPKIN
                     EEIKNKTGIDSKNYRGIRTDTSKYFGIRYEYDEEDGSITKSYYPTTKDGKLTGYKVRT
                     HPKKFNTPYGETGKDCDLILQFRFMNTNESRIVIAAGEVDALSCYQMLSDYQKGRGYE
                     PVPVVSSTIGESGGYKQIQAQYQFFSRFDKIIICADQDKTGQESVEKMAGVLPKGKVF
                     VMRLPLKDANEMLMQGKEKQFINAFFQAPAYVPSGIVGSDQLLDKIVEAAAVPKIPLP
                     PFMHKLQSLMAGGIPLGVTVCLGSASGQGKSTFVEEITYFMIFNSPHRVGVVSLESDC
                     AQYGTKLLSRHLGQKIDLIEDVDYKIQKLTSEEIRKKSDELFKHEDGTPRFHLIEERD
                     GNLEDMKELIMQLVVACECRVIILDPLQDLLDAQPLEAQSAFMSWIKGTMKSHGVTFI
                     LVNHLRKSSGGSKANSTGADIHEEDFMGHSSIFKSSACNLLFTRNKEAENEIERNTTV
                     MKMTKCRWTGRTSPFAGKYFYDNETHTLHDFDEWVANNPEKVAMDF"
     gene            1710..2579
                     /locus_tag="VFD79_04930"
     CDS             1710..2579
                     /locus_tag="VFD79_04930"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HZH93426.1"
                     /translation="MFSYDEESPTGLRWKDEYRAGVGGKPTKRVNSGHAGGISSSGYP
                     VVYVDNWPYNAKEVVWEIFNGKIPKGRSVVPIDGDPLNCKIENLELNYIIPKDAYKYG
                     EYLGQFLEYDEDSPSGLIWKKRYGKCSTAQIGSVAGSLDNGYWRIHAFGKQLKGHKIV
                     WALHNNFENQDGLHIDHIDGNPSNNNINNLRLVPEELNARNKPMNRNNTSGIHGVSFQ
                     KVRTKNGNYVERYISTWYTLEGKRRTKCFSVNVYGDELAHLLAGEYRQHQIDLLNLMG
                     AGYTERHGAKKKE"
BASE COUNT          833 a          486 c          709 g          687 t
ORIGIN      
        1 agcatgaggg aattggctat gttgaagctg tgaaagcgta tgaagatatt acaggggacg
       61 tagacacttt ttatgtaaaa ccgaaagaag gtttggagga tattgtgggt aaagagttta
      121 gtccaaagat taacgaggaa attaaaaata aaacagggat tgatagcaaa aattatcggg
      181 ggattcggac agatactagt aaatacttcg gcattcgtta cgaatatgac gaagaggacg
      241 gttctattac taaatcctac taccctacta caaaagatgg gaaattgact ggctataagg
      301 tacgcaccca tccgaagaag tttaatactc cctacgggga gacagggaag gactgtgacc
      361 tgattctgca attccggttt atgaatacaa atgaaagccg gattgttatt gctgctggtg
      421 aagtggatgc ccttagttgc taccaaatgc tgtctgacta tcagaaagga cggggatatg
      481 aacctgtccc tgttgtgtct agcactattg gggaatcggg aggatataag cagatacaag
      541 cacagtatca attcttctcc cgttttgata aaattattat ttgtgctgat caagacaaaa
      601 ctgggcaaga gtctgtggag aaaatggctg gagtgttgcc gaaggggaag gtatttgtaa
      661 tgcgtttacc cctgaaggat gccaatgaga tgcttatgca gggcaaggag aagcagttta
      721 ttaatgcgtt cttccaagca cctgcttatg tccctagcgg aattgtcgga agtgatcagc
      781 ttttggataa aatcgtggag gcagcagctg ttcctaagat tccactcccg ccgttcatgc
      841 acaaactgca aagcctaatg gctgggggga ttccgcttgg tgtcacagtc tgccttggct
      901 cggcttctgg gcaaggaaaa tctaccttcg ttgaagagat aacatatttt atgatcttca
      961 acagccctca tcgggtgggg gttgtgtcac tggagtcgga ctgcgcccaa tatgggacaa
     1021 aactgctgtc tcgccatcta ggtcagaaga ttgacctgat cgaggatgtt gattataaga
     1081 tacagaagct gacttcagaa gaaattagga aaaagtcaga cgagttgttt aagcatgagg
     1141 acgggactcc ccgattccac ttaattgaag agcgtgacgg gaatctggaa gacatgaagg
     1201 aacttattat gcagcttgta gttgcatgtg agtgtcgcgt gattatatta gacccgcttc
     1261 aagacctgct ggatgcccaa cctcttgagg cacagtctgc attcatgagt tggatcaagg
     1321 gtactatgaa aagtcacggg gtcaccttca ttctcgtcaa tcatttgcgt aaaagcagcg
     1381 gaggctctaa agctaacagt acaggggctg acatccatga ggaagatttc atggggcaca
     1441 gttctatctt caaaagcagt gcgtgtaacc ttctattcac ccgcaacaaa gaggctgaaa
     1501 atgagattga acgcaacaca actgtcatga agatgacaaa gtgccggtgg actggtcgaa
     1561 caagtccctt tgctggaaag tatttttacg ataacgagac acatacgttg catgattttg
     1621 atgaatgggt ggcgaataac ccagaaaagg ttgcaatgga tttttaagga ggtattatgg
     1681 caagacgaaa tgacattgta tgggaggatt tgttttccta cgacgaggag agtccaacgg
     1741 ggctaagatg gaaggatgag tatagggcgg gagttggagg gaagccaaca aagagggtaa
     1801 atagtggaca tgcaggggga atatctagtt ctggctatcc cgttgtctat gtagacaatt
     1861 ggccctataa tgcgaaagag gttgtgtggg aaatttttaa tggtaaaatc ccaaagggga
     1921 gatcagtggt tccgattgat ggtgacccat tgaattgtaa aatagagaac ctagaactca
     1981 attacatcat accaaaagat gcctacaagt atggtgaata tctgggacaa ttccttgaat
     2041 atgacgagga ctccccgagc gggttgattt ggaagaagag gtatgggaaa tgcagcaccg
     2101 ctcagattgg aagtgttgcc gggagccttg ataacggata ttggagaatc catgctttcg
     2161 gtaagcaact taaaggtcac aagattgttt gggccttaca taataatttt gaaaatcaag
     2221 atggactcca cattgaccac atagacggga atccctcaaa caacaacatc aataacttac
     2281 gacttgttcc agaggagctt aatgctcgga acaaacccat gaatagaaac aacacttcag
     2341 gaatacacgg agtgtctttt caaaaagtaa ggacaaagaa tgggaattat gtggaacgtt
     2401 acatttcgac gtggtacact ttagagggta agaggagaac aaaatgcttc tctgttaatg
     2461 tctacggaga tgaattggcc catcttcttg ctggtgagta cagacagcac caaatagacc
     2521 tgttaaatct catgggggct ggatatacag aaaggcatgg ggccaagaag aaagaatagg
     2581 aggatgattt gaagaagttg aatggcttcg cgcttgacct tgaggcagat ggttttgtat
     2641 ttgagtcaaa caatgtctgg tcgctgtgtc tgacagacct tgacaatcca gagaaaaaga
     2701 tcaagctcaa tcctt
//
LOCUS       DASRQF010000179         2709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_189157, whole genome shotgun sequence.
ACCESSION   DASRQF010000179 DASRQF010000000
VERSION     DASRQF010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189157"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1736
                     /locus_tag="VFD79_04935"
     CDS             <1..1736
                     /locus_tag="VFD79_04935"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HZH93427.1"
                     /translation="WYNVYEVVDMFLKKDKKSNGRIYLSIVEGYREPSTGKTKHRKVK
                     SLGYVDSLEKDFDDPISHFTELAKQMTLEAKDNAIPLSFSFSSTDTIDSSSVLRKNVG
                     FAVLSHFYHKLDIGKFFINRQRNLDLKYSLNNIFQMLVYSRVLIPCSKKSSFESMDRY
                     FLDFKFSLDHVYKALDHFYAFKDDLLLHLHEMVRMHYGRDTSNVYYDVTNYYFEINES
                     DDFRKKGVSKENRTNPIVQMGLLMDNNGLPITYRLFEGNTNDCTTLMPVLDDLKDDYN
                     LGRVIVVADKGLNTGENIAYNIIHKNGYIYSQTVRGANAELKEYVISDEGYVSSEDGF
                     RIKSRVVSTKIWVENDKGKRVQVPIEQKQVAFYSPDYAKRAKYEREKAVEKARKLILG
                     SKTGKLPEKGAKKYILSTPCDVETGEVYELSKYHFIDSSKISEEEKYDGYYVIVTSEL
                     NMPNEKILDAYRNLWKIEETFKITKSELKTRPVHVSTREHIEAHFLTCFVSLLILRLL
                     QLETKGKHSVRTLVDTMKEMTGTYVDKNYYMFDHYNEIVDDLGKATDIDFSRRFMTLG
                     DVKNIIAKTKK"
     gene            complement(2262..>2709)
                     /locus_tag="VFD79_04940"
     CDS             complement(2262..>2709)
                     /locus_tag="VFD79_04940"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZH93428.1"
                     /translation="QSTVAEEAVINLDRRYVEGETLQSVIQEYQDIADEMKRNDPTFE
                     IEISRTPSSVSVLPHPPLNTGLDESIVLAVKEAIEDVIGYEPELKTEYGWTDGALISK
                     YMKVPTVVVGPGDIALAHTSEERIGINDLVRGVELYTQIADRFCVF"
BASE COUNT          821 a          516 c          529 g          843 t
ORIGIN      
        1 tatggtataa tgtatatgag gtggttgaca tgtttttgaa aaaggataaa aagtctaatg
       61 gcagaatata tctatctatt gttgagggtt atagggagcc ttcaactggg aaaaccaagc
      121 atagaaaggt taaatctctt ggatatgtgg attccctgga gaaggatttc gacgatccta
      181 tctctcattt tactgagctt gccaagcaaa tgactcttga agctaaggat aatgccatcc
      241 ctttgagttt tagcttttca tcgactgata ctattgattc aagcagtgtg ctaaggaaaa
      301 atgttggttt tgctgtactt agtcattttt accataagtt ggatattggg aagttcttca
      361 tcaacaggca aagaaacctt gatctcaaat attctcttaa caatattttt cagatgctgg
      421 tctattccag ggtgctgatt ccttgttcca agaagtcttc ttttgaatcc atggacagat
      481 attttctgga cttcaagttc agtttggacc atgtgtacaa agctcttgat catttctatg
      541 cttttaagga tgatctgctc cttcatcttc atgaaatggt gagaatgcat tatggcagag
      601 atacaagcaa tgtctattac gatgttacca attactactt tgagataaat gaatctgatg
      661 atttcaggaa aaagggtgtt tccaaggaga ataggaccaa tccaatagtc cagatggggc
      721 ttttgatgga taacaacgga ttgcccatca cttaccgatt gtttgaggga aacaccaatg
      781 attgcaccac cctgatgcct gttttggatg acttaaagga tgattacaat cttggccgtg
      841 tgattgttgt ggctgacaag ggacttaaca ctggtgagaa tattgcctac aacatcatac
      901 acaagaatgg atacatctat tcccagactg ttagaggggc caatgcagaa cttaaggagt
      961 acgtgatttc tgatgaagga tatgtttcat ctgaggatgg tttcaggata aagtcccgtg
     1021 tggtttccac taagatatgg gttgaaaatg ataaaggcaa aagggtccag gttcccattg
     1081 agcagaagca ggtggctttc tacagccctg attatgccaa aagagccaaa tatgaacggg
     1141 aaaaggctgt tgagaaggcc aggaagctaa ttctaggaag caagactggt aaacttcctg
     1201 aaaagggagc taagaagtat atcctctcta ccccttgcga tgtggagaca ggagaggtct
     1261 atgaactatc gaaatatcac tttattgaca gttcaaaaat atctgaggaa gaaaaatatg
     1321 atgggtatta cgtgatagtg acaagcgagc tgaatatgcc aaacgagaag atacttgatg
     1381 cttacagaaa cctttggaag atagaggaaa cattcaaaat cacaaaaagt gagttaaaga
     1441 caagacctgt tcacgtctcc acaagagagc acattgaagc ccatttcctg acctgctttg
     1501 tctctctgtt aatccttagg ctgctacaac tggaaacaaa aggcaaacac tcggtccgaa
     1561 ccttagttga cacaatgaag gagatgactg ggacctatgt tgacaagaac tattacatgt
     1621 ttgatcatta caacgagatt gttgatgatt taggtaaagc aacagacata gatttctcaa
     1681 gaaggttcat gacactaggt gatgtcaaaa atatcatagc caagaccaag aaatgacctg
     1741 tagtactaca actttatgac agtgttaaaa agcctgtgtc cctctattta caagggacac
     1801 aggctctatt ttttgtgttt tggcgctaaa ctcaagagtg ctccaccttc tcttattctt
     1861 aaataccttt tcataattat ccctccaaca ggtaataatc aaattccctc tatacaagtc
     1921 attaacatga aatcattact tttctattaa gtttattata tcatcatttg ttcattttag
     1981 aagcttttga aaaataaaag tttacataac attacttaat gagttgatac ccacaataat
     2041 gacatgtaaa ctcgtatttt ccaatgtttt gatgattata acttttgtca taaatgatgc
     2101 cagatacttt ccatctcaat ttaatttcca tattcacaag ttcttatata tcataatcgg
     2161 tagcgtttct ctttaccatt cgcttgtcac aatctttcaa ttgtgatctt aggaataccc
     2221 gttaccctgc atattgtctt attttctcat tgatacttcc actaaaacac gcagaaacgg
     2281 tctgctatct gtgtatataa ttctactcct ctaacaaggt cattaatacc aattctttct
     2341 tcggatgtat gggctaaggc aatatcccca ggacctacaa ccactgtcgg tactttcata
     2401 tattttgaaa tcagcgcccc atctgtccat ccatactcgg tctttaactc cggctcatat
     2461 cctattacat cttctatggc ttcctttaca gctagtacga tcgactcgtc cagaccggta
     2521 ttaagaggcg gatgaggcaa aactgaaaca cttgagggtg tacgactgat ttctatttca
     2581 aatgttgggt catttctttt catctcatcg gcgatatcct ggtattcctg aattacagac
     2641 tggagggttt caccttcaac atagcgtcga tctaggttta ttaccgcttc ttctgcaacg
     2701 gttgattgg
//
LOCUS       DASRQF010000180         3241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1251579, whole genome shotgun sequence.
ACCESSION   DASRQF010000180 DASRQF010000000
VERSION     DASRQF010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251579"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VFD79_04945"
     CDS             <1..855
                     /locus_tag="VFD79_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M20"
                     /protein_id="HZH93429.1"
                     /translation="DEAQGEVTVPPTSLKQTDTKVGYTVQTPLAAYSYYNFFTHSMSP
                     KDVILKMKEKAEEAFEEVLERMDASYRRYCEMGGHTYTPFPYRRRVYTWEDFYNELLT
                     IHDDKFKLAIRNFAADLNEKNPSMDLRDFSVAVIEEALKWSQDKSPVVIIYYSSLFSS
                     RIEMTGKTDKEKALLDSVKRSIDLVQDHSDKPIVVKMFYPYISDSSFMALSDDPHELV
                     ALEKNMPSWGTKYNHPIEDIMDINVPVVNIGTFGKDGHKFTERVHMKYTFEHVTNMTF
                     NTIRSLLS"
     gene            937..1779
                     /locus_tag="VFD79_04950"
     CDS             937..1779
                     /locus_tag="VFD79_04950"
                     /inference="COORDINATES: protein motif:HMM:NF025701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HZH93430.1"
                     /translation="MYRRAITFMIIFTLILSSGSVALAGPIEKLDGHWSRDLIDDGFT
                     SRYFPYLAKENFKGLSPDGTISRENFTLSLASLFRDMGYTVDGIAAPGTLARSGMVDV
                     LGDRLVQIGMTSGQDHQMPFGDVSYLQVKTQNNLRLLHREGIITGEKDSVFNPARNLT
                     QAEAIVVLQRTQSAIREWKDISFRTLGVTQNYSNQEEVTTRLSENVVTLVVTKEFPTP
                     GYTLRIDRIVREADHYRVDFTISPPEPGTILPQVITYKTITVEIDREELGEPPYDFAV
                     EGFR"
     gene            1895..>3241
                     /locus_tag="VFD79_04955"
     CDS             1895..>3241
                     /locus_tag="VFD79_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZH93431.1"
                     /translation="MKSVMTGNQAIARGFWEAGGRLAASYPGSPTVEMLESLKEYDEI
                     YSEFSVNEKVALEVAMGASFYGARAMASMKHVGINIAADPLMTFTQTKINGGFVLVSG
                     DDPGMASSQNEQDNRIFGKFANMGILYPGNSQEAKDYMKIGLEMSENFQMPILLDITS
                     RVCHSRSVVQTGDRVEREIKGFTPDREKYTMLPPHTFKPQYQMKERIEKLAETAYDIP
                     VNELFEKEGTDTLLITSGLMYYNLLELDPKVSIYKLGLVYPLSEKRLRELADRYKNII
                     VLEEMMPFIENELTLMGISCRGKDLFSFTGELHIEDIRRGLQKAGVMEEEYESKAPKE
                     DAVPRPPMFCAGCPHRPVFDVLKKAKVRVIGDIGCYSMAALDQWDASDIMISMGASLG
                     ITKGMNKMMRRNGDEAPLVSVIGDGTFFHSGMTGFVNLLHQMDEDDNMTFIILENST"
BASE COUNT          944 a          714 c          847 g          736 t
ORIGIN      
        1 gacgaagccc agggagaggt gaccgtacct ccaacaagcc ttaagcagac agatacaaag
       61 gttggctaca ctgtccagac ccctcttgca gcctacagct actacaactt cttcacccac
      121 agcatgagtc caaaagacgt catccttaag atgaaggaaa aggctgagga ggcctttgaa
      181 gaggtgctgg agaggatgga tgcttcctac aggagatact gtgagatggg tggacacacc
      241 tatacaccat tcccatacag gagaagggta tacacctggg aagacttcta caatgagctc
      301 cttacaatcc acgatgacaa gttcaagctt gcaataagga attttgcagc tgaccttaac
      361 gagaagaacc cttcaatgga ccttagggac ttctcggtgg ctgtgataga ggaggcattg
      421 aagtggtccc aggacaagtc cccggtggtt ataatctact attcgtccct tttctcatcc
      481 aggattgaga tgaccggaaa gacagacaag gaaaaggctc tgttggattc agttaagagg
      541 tccatagacc ttgttcagga ccactcagac aagccgatag tagtgaagat gttctaccca
      601 tacatctctg actcgagctt tatggccctg tcagatgacc cccacgagct ggtggccctt
      661 gagaagaaca tgccatcatg gggaacgaag tacaatcacc caatagagga tatcatggat
      721 ataaatgttc ccgttgtcaa catcgggacc ttcggcaagg acggacacaa gttcacagag
      781 agggtccata tgaagtacac ctttgagcat gtaaccaata tgacctttaa cacaataagg
      841 agccttttga gctaaaattt cataatgtaa cagaattgca aacatagtcc ctccttccct
      901 gggtatatag actacaggga agggggatta ttttttatgt atagaagggc aattactttt
      961 atgataattt ttacattgat actttcatca ggatcggttg ccttggctgg gccaattgaa
     1021 aagcttgatg gtcactggtc cagggatttg atagatgatg gatttacaag ccgttatttt
     1081 ccttatcttg ccaaggagaa cttcaagggg ctcagtcctg atgggaccat ttccagggag
     1141 aacttcaccc tgtccctagc ctcccttttc agggacatgg gctacacagt ggatggaata
     1201 gcagctccag ggacccttgc aagaagtgga atggtcgatg ttctgggaga caggctggtt
     1261 cagattggaa tgacaagtgg ccaggaccac cagatgccct ttggggatgt atcctacctg
     1321 caggttaaga ctcaaaacaa tctcagactc ctccacaggg aaggaataat aaccggtgag
     1381 aaggacagtg ttttcaatcc ggccaggaac ctgacacagg ctgaagccat tgtagtgctt
     1441 cagagaaccc agtctgcaat aagggaatgg aaggacataa gcttcagaac acttggcgta
     1501 acacagaact acagcaacca ggaggaagtg accacaaggt tgtctgagaa tgtggttaca
     1561 cttgtggtga caaaggaatt cccaacaccg ggctacacct taaggataga cagaatagtc
     1621 agggaagctg accactacag ggttgacttt accattagtc cgcctgaacc gggaacaatt
     1681 ctaccccagg tcattaccta caagaccata acagtagaga tagacaggga ggaacttgga
     1741 gagcctccat acgattttgc agtggagggt ttcaggtaga aaaaaagcat tgtagaccgc
     1801 aggctaaccg cctgcggttg tttttgcgct caaatttagc gctttaacag gggtgtggaa
     1861 tatggtaaaa tctaggtatg agggagggat attcatgaaa agtgtaatga cgggaaacca
     1921 ggcaatcgca aggggcttct gggaggcagg cgggcggttg gccgcaagct atccagggtc
     1981 tccgactgtt gagatgctgg agagtctaaa ggaatacgat gaaatataca gtgagttttc
     2041 agtgaacgag aaggtggccc ttgaggttgc catgggtgca tccttctacg gggcaagggc
     2101 catggccagc atgaagcatg ttgggattaa cattgctgcc gaccccctga tgaccttcac
     2161 ccagacaaaa atcaacggag gctttgtcct ggtatcaggg gatgatccgg gaatggccag
     2221 ctcccagaat gagcaggaca acaggatatt cggcaagttt gccaatatgg ggatcctcta
     2281 ccctggcaac agccaggagg caaaggacta tatgaagatt ggcctggaga tgagcgagaa
     2341 tttccagatg ccaatcctcc ttgacatcac atcaagggta tgccacagca ggagtgttgt
     2401 ccagaccgga gacagggttg aaagggaaat caagggcttt acaccagacc gggagaagta
     2461 cacaatgcta ccaccccata ccttcaagcc acagtaccaa atgaaggaga ggattgaaaa
     2521 gcttgctgaa accgcctatg acatacctgt aaatgagcta tttgagaagg aggggactga
     2581 taccctccta ataacatccg gactcatgta ctacaacctt cttgagcttg atccaaaggt
     2641 atcaatatat aagctgggtc tggtctatcc actttccgag aagaggctaa gggagcttgc
     2701 tgacaggtat aaaaacataa tcgtacttga ggaaatgatg ccctttattg aaaacgagct
     2761 gacccttatg ggaatatcct gcaggggcaa ggatcttttc agctttacag gagagctaca
     2821 tattgaggat ataagaagag gactccagaa ggcaggggtt atggaagagg agtatgagtc
     2881 taaggctcca aaggaggatg ccgttccaag acctccaatg ttctgtgcgg gctgtcccca
     2941 caggccggtc tttgacgtgc tgaaaaaggc caaggtcagg gtcatagggg acatcggctg
     3001 ttattccatg gctgccctgg accagtggga tgcctcggat ataatgatct caatgggagc
     3061 ctcccttggg atcacaaagg gcatgaacaa gatgatgagg agaaacggtg acgaagctcc
     3121 tcttgtttca gtgataggtg atggaacctt tttccactcc ggcatgaccg gctttgtaaa
     3181 tctcctccac cagatggacg aggatgacaa tatgaccttc ataatcctgg aaaactcaac
     3241 c
//
LOCUS       DASRQF010000181         3104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1282906, whole genome shotgun sequence.
ACCESSION   DASRQF010000181 DASRQF010000000
VERSION     DASRQF010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282906"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1763)
                     /gene="dnaK"
                     /locus_tag="VFD79_04960"
     CDS             complement(<1..1763)
                     /gene="dnaK"
                     /locus_tag="VFD79_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HZH93432.1"
                     /translation="MSKIIGIDLGTTNSAVAVMEGGEAVIIPNAEGNRTTPSVVAFTK
                     DGERLVGETAKRQAITNTERTIQSIKRHMGSDRKDEIDGKSYSPEEISAMILQKIKAD
                     AESYLGETVTDAVITVPAYFTDSQRQATKDAGKIAGLNVKRIINEPTAAALSYGLDKE
                     HGQHKIMVFDLGGGTFDVSILELGDGVFEVLATRGNNHLGGDDFDDKLIDYIAEQFKK
                     ENGVDLKADKMALQRLKEAAEKAKKELSTTKSTNINLPFITATAAGPQHLNMDITRAK
                     FDELTSGLVEMTIEPVKAALKDAGLTAADIEKVLLVGGSTRIPAVQDAVKKLTGKDPQ
                     KDINPDESVALGAAIQGGVLSGEVKDLLLLDVTPLSLGIETLGGVTTKLIERNTTIPT
                     KKSQVFTTASDSQTSVDIHVLQGERQMANDNVTLGRFQLTGIPPAPRGVPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITASTKLSEDEINEKVKEAERFAEEDKVKKEKIEVR
                     NNGDSIVYQTEKTLKDLEGKIEADEKAKVEEKLEALKKALEGDDIDDIKTKTEELTNE
                     FHAISQKLYETAQGEGGEGQEA"
     gene            complement(1782..2336)
                     /gene="grpE"
                     /locus_tag="VFD79_04965"
     CDS             complement(1782..2336)
                     /gene="grpE"
                     /locus_tag="VFD79_04965"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HZH93433.1"
                     /translation="MTKEDNNIKEDERDLLEEESNGTEVPQEEQEDDPIFKSKAMLEE
                     KENQIIDLNNKLTRLQADFQNFKRRTEKDRENSITYGIEAIILDLLPVIDNFQRAIAA
                     QDNKEDGFYKGVSMIEKQFIDVLERNSVQEIEALGKEFDPNLHHAVSVVESEEEEGTV
                     IEVLQKGYVLKDKVIRPCMVVVAK"
     gene            complement(2343..>3104)
                     /locus_tag="VFD79_04970"
     CDS             complement(2343..>3104)
                     /locus_tag="VFD79_04970"
                     /inference="COORDINATES: protein motif:HMM:NF013768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HZH93434.1"
                     /translation="ALERESKELDQLIQNSARLLSQITSYTALALSPQIKETRIKYVQ
                     LLPMDDHQVLLILVNENGLVKNAVFKTDQTIPENQLVAITNMLNERLKNKSFNQLNNE
                     SMKEMFNQFYNYKNEIERLIPIINKSVESMEDIDLYADGIGRLLNFPEYKDIDKVKSL
                     MSFIEDKDQLLNILLMDSITRDVHISIGHENIYAPLKDCSIITATYRLEGETVGKIGI
                     IGPTRMDYLGLIKTLRIFSDNISEIINNYNGSVKR"
BASE COUNT          667 a          770 c          573 g         1094 t
ORIGIN      
        1 gcctcttgtc cttctcctcc ctctccttga gcagtttcat acagcttctg tgaaattgcg
       61 tggaactcgt tggtcagctc ctcggtcttg gtcttgatat catctatgtc atcaccctca
      121 agtgctttct taagagcttc caacttttct tcaaccttgg ctttttcatc agcttcaatt
      181 tttccttcaa ggtccttcaa ggtcttttct gtttgataga ctattgaatc accgttgttt
      241 ctaacctcga tcttttcctt ctttaccttg tcttcctctg cgaacctctc agcttcctta
      301 accttttcat ttatctcgtc ttcactcagc ttggttgatg ctgtaattgt gatcttctgt
      361 tccttgcctg ttccaaggtc cttggcacta acatttacaa taccattggc atctatgtca
      421 aaggtaacct cgatttgtgg aactcctcta ggtgcaggtg gtatcccagt cagctggaac
      481 cttcctaggg ttacattatc gttggccatc tgacgctcac cctgcagcac atgaatatca
      541 actgatgtct ggctgtccga tgcagttgta aacacctgtg atttcttggt tggtatggtt
      601 gtatttcttt caatcaactt ggttgtaacc cctccaagtg tctcaattcc cagtgaaagg
      661 ggtgttacat ctagaagcaa gaggtctttt acctcaccac tcagaactcc tccctgaatt
      721 gccgctccaa gagcaacact ctcatcaggg tttatatcct tctgtgggtc ttttccagtg
      781 agctttttga ctgcatcctg tacagctggt atccttgttg acccaccaac cagcaatacc
      841 ttttctatgt cggctgcagt aagtccggca tcctttaatg ctgccttgac aggttcaatt
      901 gtcatttcaa caagtcctga tgtcaactcg tcaaacttgg cccttgttat atccatatta
      961 aggtgttgtg gtcccgctgc agttgcagtg ataaatggaa ggtttatgtt ggttgacttg
     1021 gttgttgaaa gttccttctt agccttctca gcggcttcct tcagcctctg gagtgccatc
     1081 ttgtctgctt tcaggtcaac tccgttttcc ttcttgaatt gttcagcaat gtagtctatc
     1141 agcttgtcgt caaaatcatc tccgccaagg tggttgtttc ctcttgttgc aagtacctcg
     1201 aacactccat ctccaagttc aagtatggat acgtcgaatg ttcctccacc taggtcaaat
     1261 accatgatct tgtgctggcc gtgttccttg tccaaaccat atgacaatgc agcagcagtt
     1321 ggctcattta tgattctttt tacattaagt cctgcgattt tccccgcgtc cttggttgcc
     1381 tgtctttggc tatcagtaaa ataggcaggt actgtgataa cagcatctgt tactgtttct
     1441 ccaagatagc tctctgcatc agccttgatc ttttgaagaa tcattgctga tatctcttca
     1501 ggactgtagg atttaccatc tatctcatcc ttgcggtcgc ttcccatgtg tctcttgatt
     1561 gactgtattg ttctctctgt atttgttatt gcctgccttt tagctgtttc ccctacaagc
     1621 ctttccccgt ccttggtgaa tgcaaccacg gaaggagtcg ttctgttccc ttcggcatta
     1681 ggtattatta cagcctctcc accttccatt acggcgactg ctgagtttgt tgtacccagg
     1741 tcaattccaa ttattttgct cattccatat tcctccttaa attattttgc aacaacgacc
     1801 atgcatggtc tgataacttt atcctttaaa acataaccct tctgtaagac ctcaattacc
     1861 gtgccctctt cctcttcgct ttcaacaact gatacggcat ggtggagatt tgggtcaaat
     1921 tccttcccca aggcttcaat ctcctggact gaattccttt ccaacacatc tatgaattgc
     1981 ttttcaatca ttgatactcc cttgtagaag ccgtcctcct tgttgtcctg ggcagcaata
     2041 gcccgctgga agttgtcaat tacaggcaga aggtccagga taatggcttc aataccatag
     2101 gttattgagt tttccctgtc cttttccgtt ctacgcttga agttctgaaa atcagcctgt
     2161 aacctggtca gcttattgtt cagatcaatg atttgatttt ccttttcttc caacattgcc
     2221 tttgatttaa aaatgggatc atcctcctgt tcttcctgag gaacttccgt cccgttggat
     2281 tcttcctcca gcaggtccct ttcatcctcc ttgatattgt tgtcctcttt tgtcatttgc
     2341 aatcacctct taacacttcc attatagtta ttaataatct cggaaatatt atccgagaat
     2401 atcctcaagg tcttgataag cccaaggtag tccattctgg ttgggccgat tattccaatt
     2461 ttccctacgg tttcaccttc aagcctgtag gtggcagtta ttatgctgca atccttaagg
     2521 ggtgcataga tattttcatg acctatgctt atgtgcacgt ctcgcgttat ggaatccata
     2581 aggaggatat tcaaaagctg gtccttgtcc tctataaatg acattaggga ctttaccttg
     2641 tctatatcct tgtattcagg gaaattcaac agtctcccta ttccatcggc ataaaggtca
     2701 atatcttcca tgctttcaac ggacttgttg atgatgggaa taagtctctc aatctcattc
     2761 ttgtaattat agaactgatt gaacatttcc ttcatcgact cattgttaag ttgattgaag
     2821 gacttatttt tcaatctttc atttaacatg ttggttattg ccacaagctg gttttccgga
     2881 atggtctggt cagtcttgaa aactgcattc ttcaccagtc cgttttcatt gaccaatatg
     2941 agcaggactt ggtggtcatc catcggcagc aactgaacat actttatcct tgtttccttg
     3001 atctgaggtg acaatgccaa agctgtatag cttgttatct gagaaagcag cctggctgag
     3061 ttttgaatca actgatccag ttccttgctt tccctttcca gggc
//
LOCUS       DASRQF010000182         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_732380, whole genome shotgun sequence.
ACCESSION   DASRQF010000182 DASRQF010000000
VERSION     DASRQF010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732380"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VFD79_04975"
     CDS             complement(<1..601)
                     /locus_tag="VFD79_04975"
                     /inference="COORDINATES: protein motif:HMM:NF014750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HZH93435.1"
                     /translation="MAVSYGWAGKVLRVNLSNGKITTEDTGKYKDYIGGMGIGYKVLF
                     DEVPVGTHPHDAKNKIVFGTGPLTGSGAPCSSRTNVTSLFPTNPKHMVADSHMGGNFA
                     ASMKYAGYDAIIVEGKARGPVWLRIEDDKVAIEDASKMWGQGIYNTTALVASEMGKEA
                     SIAAIGQAGENLVNLSVIMNGYSHSAGGHGSILGAKKLKA"
     gene            complement(623..1282)
                     /locus_tag="VFD79_04980"
     CDS             complement(623..1282)
                     /locus_tag="VFD79_04980"
                     /inference="COORDINATES: protein motif:HMM:NF007382.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HZH93436.1"
                     /translation="MEEKSKKKFLERETTRREFLKLTGKGFGGAVISMSILSMLGCES
                     EQTVAYALPSGLLVADASRCTGCQRCEISCTSIHDEKVQPFISRIKVSENYNFGNDGP
                     RIDYYRQNGQFGNLSMSPETCKQCRDPYCGRACPAGAIYSDPKQGNARVVDASKCIGC
                     GACEEACPWHLPTVDPETKKSTKCVACGFCAENCPTGALKMIPWEDVKKAMRRNGYRF
                     S"
     gene            complement(1476..>2086)
                     /locus_tag="VFD79_04985"
     CDS             complement(1476..>2086)
                     /locus_tag="VFD79_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HZH93437.1"
                     /translation="AIAKKYDVATFETLTGFKNICAKANEWDETGAFSFVFGYEESIG
                     YVYKDLVRDKDAVVSSMIIAEMAGFYKKEGRSLFDVLEDIYKEFGYYYEKLISIVLEG
                     VEGQERIKRMMDDIRHHSIDSIGEMMLQKTVDFMRDETDAGRSNVLKYYLNDGSWYAV
                     RPSGTEPKIKLYIYSKGKSREDAKEKVELIEKVVRHRMESVE"
BASE COUNT          522 a          552 c          340 g          672 t
ORIGIN      
        1 ttgccttaag cttttttgcc ccaagtatgc taccatgacc tcctgccgag tgtgagtagc
       61 cgttcataat tactgaaagg ttgacaagat tttcaccagc ttgaccgatt gcagctatac
      121 ttgcttcctt acccatctcc gaagccacaa gagcagttgt gttgtagatt ccctgacccc
      181 acatttttga ggcatcctct atggcaacct tgtcatcctc tattctgagc cacaccggac
      241 ccctagcctt accttcaact attattgcat cgtagccagc atacttcatt gatgctgcaa
      301 aattccctcc catatgggaa tcagctacca tatgcttcgg attcgttgga aacaaggatg
      361 ttacatttgt tctggaacta cagggggccc ctgatccagt taggggacct gtaccgaata
      421 caatcttatt cttagcgtca tgaggatgag tccccacagg aacttcgtca aacaacacct
      481 tgtaacctat tcccatacca cctatgtaat ccttatattt accggtatct tcagttgtga
      541 tctttccatt ggacaggttg actcttagaa cctttcctgc ccatccgtat gaaaccgcca
      601 tgttcttccc tcctttgatc aattaagaaa acctatatcc atttcttctc attgccttct
      661 tgacatcttc ccatggaatc atctttaaag ccccagttgg acagttctca gcacagaatc
      721 cacaagccac acattttgtg gatttcttgg tctcaggatc aactgtaggc aaatgccatg
      781 gacatgcctc ctcgcaggca ccacaaccaa tgcactttga agcatccaca acccttgcat
      841 taccttgctt tggatcagaa taaatagcac cagccggaca tgctctacca cagtatgggt
      901 ctctacactg cttgcaggtt tcaggactca ttgagagatt cccaaattgt ccattctgtc
      961 tgtagtaatc aattctagga ccgtcatttc cgaaattata gttctcagat accttgattc
     1021 tggatatgaa tggttggacc ttttcatcgt gtatggaagt gcaagatatt tcacacctct
     1081 gacatcccgt acaccttgaa gcatccgcta ccaaaagtcc gcttgggagt gcataggcaa
     1141 cagtctgctc actttcacag cccagcatac tcagtatgga cattgatatg accgctcccc
     1201 cgaagccctt tcctgttagt tttagaaact cccttctagt ggtctcacgc tccagaaatt
     1261 tcttttttga cttttcctcc atgtttaccc tcctttgttt gttatttctc aaatatctta
     1321 tggcaaatat aaaaccatcc ttggcagcat ggtttatgtt aatattttat catattctta
     1381 cactatttca acaaaataag ccaaatacac atttaaaaca tatatccatt ttcttcgcat
     1441 aataaaggcg gggatttttc cccgccttta ttatgctatt caactgattc cattctgtgt
     1501 cttacaacct tctctataag ctctaccttc tccttggcat cctctctgga tttacccttg
     1561 gagtagatgt aaagctttat ctttggttca gttccagatg gtcttaccgc ataccatgat
     1621 ccgtcattca ggtagtattt caatacattt gatctacctg catctgtctc atctctcatg
     1681 aagtctacag ttttttgcag catcatttct ccaatggagt ctattgaatg atgtctgatg
     1741 tcatccatca ttctctttat cctctcctga ccttccactc cctcaagtac aatggagata
     1801 agcttctcat agtagtatcc aaactccttg tatatgtcct caagaacatc aaagagtgat
     1861 cttccttcct tcttgtagaa gcctgccatc tcagctatta tcattgagga tacaacagca
     1921 tccttatccc ttacaaggtc cttgtagaca taacctatac tttcctcgta tccgaacaca
     1981 aagctgaagg cccccgtttc atcccactca ttagcctttg cgcagatgtt cttgaagcct
     2041 gtcaaggttt caaatgttgc aacatcatat ttcttggcga ttgcct
//
LOCUS       DASRQF010000183         5176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1847529, whole genome shotgun sequence.
ACCESSION   DASRQF010000183 DASRQF010000000
VERSION     DASRQF010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1847529"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /locus_tag="VFD79_04990"
     CDS             <1..1239
                     /locus_tag="VFD79_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93438.1"
                     /translation="GGNEGLPYWIMSLITVVTLPILPLVLASVLIMVFMKYTNIKGKK
                     DLIRVISAVFFIIFIIFVQIKINQLTSQNLMESESFMFNLARDANLLLNQIGIAFPTA
                     MWGALALSNGFSPSGILYILLFAGTGVVGYVLMILLAERIFFDGLIGNIEVSARKGRG
                     RTGDSAGRIRAGNPIITMAKKELLMIFKTPVYLMNSVGGVIILPVLLAISMLTGGEAY
                     EPMKDMIMENREVLVLGSIGIIASFGILNSLGATTFSREGTNMWIQRTLPIRPKVQIV
                     GRTLSSISLQVLGGLLLVGGIYFIVPLRIHEVIIILLFGVCASIPLTMIGMAVDILRP
                     MTGWTNPQQAMKQNLNVIISMAIGTLFIFGAGFLGYILLPNYGIWTILGVYAVIIVTA
                     TGLLYKLLENMISSKFINME"
     gene            complement(1285..1884)
                     /gene="rpsD"
                     /locus_tag="VFD79_04995"
     CDS             complement(1285..1884)
                     /gene="rpsD"
                     /locus_tag="VFD79_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HZH93439.1"
                     /translation="MANMKGPRFKLSRRLGLNVYGHPKAMDRAVKGTSRADKKLTEYG
                     QQLLEKQRLRSYYGVMEKQFVKYVVKAKKSEDQTGHALVKILESRLDNLVYRLGIASS
                     IRQARQMVVHGHILVNDKKVDIPSYLCEPGDTVTLREKSRSVELFKDNFDNALAHSLP
                     YLSKDADLFGGTFERLPEREEVPIEIEDQLVVEYYSKLL"
     gene            complement(1983..2483)
                     /locus_tag="VFD79_05000"
     CDS             complement(1983..2483)
                     /locus_tag="VFD79_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HZH93440.1"
                     /translation="MDYIVKAFIEIPKGSSNKYEYNKEFKVFELDRSLFSPMFYPTDY
                     GYIPDTLAEDGDPIDIMVLIANPTFPGCLVHARVIGMFLMKDEKGKDEKIIAVPRNDP
                     RFTEIETVDDMGAHMKKEFEHFFSEYKRLEEKEVIVSGWAGIDEAIEAIEKSRIAYKE
                     AKDALR"
     gene            2699..3499
                     /locus_tag="VFD79_05005"
     CDS             2699..3499
                     /locus_tag="VFD79_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HZH93441.1"
                     /translation="MLKLYISADIEGVTGVTHWDETSKNKGDYSQFADQMTAEVRAAC
                     EGAGEYAEVEIVVKDAHESGRNIDHSKLPHYTRLIRGWDEGLYSMVQGLDDSYDGLAF
                     IGYHSGAGIEGSPLSHTLTPGILSIRINGQAVDEFMLHAYIAAYHKVPVVFLSGDQAL
                     CKRVKEINPNIETVAVKEGRGSSTISIHPDRAVELIRSGIKRGLAMEKNMLLIQLPET
                     FQVEVEYPRHYEARRNSFYPGASLVDSTTVSFTSNDYIEVLRFIHFTV"
     gene            3515..4891
                     /locus_tag="VFD79_05010"
     CDS             3515..4891
                     /locus_tag="VFD79_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZH93442.1"
                     /translation="MTTITIDDKKVRKKILSMTLPITAENILQMTAGVVSMAMVGRID
                     SVAVGAIGMSNILFRIIWAVLKGIGTGNSVFVAQSFGAGNHGKLKDTTEQAFILALGI
                     SMIFQQLIYWNTESLIGIFNPTPALLAGGTMYLRILSWSLPFTAIILLTAGILQGMGN
                     AKTPMIIVGILNGVNIVFSYVLIFGKFGLPELGLRGAAIAYNISYGIAALMGLIVLFG
                     KNGTFAKLGGGFKLRFNRTQSIRLIRFAIPTSFEMSFWQFASIIITRAVLTYGEVAYA
                     AYQLGLQAESISYMPAAGFGIAASTFVGQSVGSRDSEMGKTYMKHLIRLSLVVTSVAG
                     GLMIVLPSQIMRAFSNDPEIIAVGAVYLFIMGLLQIPQNLAGLLNGALRGAGYPKASM
                     INVGVGLWLVRVPLVLFMTYVMDAGIEWIWIMMGVDLLVRFILAVFTYRRKDIFNSEK
                     EAVGSIFL"
     gene            4901..>5176
                     /locus_tag="VFD79_05015"
     CDS             4901..>5176
                     /locus_tag="VFD79_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZH93443.1"
                     /translation="MVLRIKNVDIVTIDQENRIIENSNIYIEDGIITHIGDEVLGIEV
                     KDTIDGRNKAALPGLVNAHTHMGMSLLRNYADDLPLHQWLTEKIWPVE"
BASE COUNT         1522 a          952 c         1133 g         1569 t
ORIGIN      
        1 ggagggaatg aagggcttcc atattggata atgagcttga taacagttgt aacccttcct
       61 attctacccc tggtcctggc atcagtattg attatggtat tcatgaaata cacgaatatt
      121 aagggtaaaa aggatcttat aagagtaatt tcagcagtat tctttatcat atttatcatt
      181 tttgtgcaga taaaaataaa tcagttgaca tcccaaaatc tgatggaaag cgagagcttt
      241 atgttcaacc tggcaaggga tgcaaatctt ttgctaaacc aaataggtat tgccttccca
      301 actgcaatgt ggggagcttt agccttgtcc aatggctttt ctccatctgg aatattatat
      361 atactattgt ttgcaggaac tggtgtggtt ggatatgtac ttatgattct tttggctgag
      421 agaatatttt ttgacggatt gattggaaac attgaagtat cagcaaggaa gggaagagga
      481 agaactggag actcagcagg aagaattaga gcaggcaatc caatcatcac aatggctaaa
      541 aaagagctgt taatgatttt caagactcca gtttatctta tgaattcggt tggaggagtc
      601 ataattctac ctgttctttt ggccatatcc atgcttaccg gtggtgaggc atatgagcct
      661 atgaaggata tgataatgga aaacagggaa gtactagttt taggatccat aggaatcata
      721 gcttcctttg gaattctgaa ttcattgggg gcaacaacct tttcgagaga aggaacaaat
      781 atgtggatac aaaggacact tccaatcagg ccaaaggttc agatagtggg gagaactcta
      841 tcctcaattt ctttgcaggt tttgggagga ttactcctgg taggtggtat ttactttatt
      901 gtacccttaa gaatccatga agttataata atattgttgt ttggtgtatg cgccagtata
      961 cccctaacca tgataggaat ggctgttgat attcttagac caatgactgg gtggaccaat
     1021 ccccagcagg ccatgaagca gaatctcaat gttataatat caatggcaat aggcaccctt
     1081 ttcattttcg gagcaggatt cctgggatat attttgctgc caaactatgg tatttggacc
     1141 attttagggg tctatgctgt cataatagtg actgcaacag gacttcttta caagctgctt
     1201 gagaatatga tttcttcaaa attcataaat atggaataaa aaaaaggaag gtctcaaatc
     1261 agagtccttc catttcatat gtgcctataa aagctttgag taatactcaa cgacaagctg
     1321 gtcttctatt tcgataggca cttcctctct ttcaggaagt cgctcaaagg ttccaccgaa
     1381 aaggtctgcg tcctttgata aatagggaag agagtgtgct aaagcattgt cgaaattatc
     1441 cttgaaaagc tcaacgcttc tggatttttc tcttaaggtt actgtatcgc caggctcaca
     1501 aagatatgat ggtatatcaa cctttttgtc atttacaaga atgtgaccgt gaacaaccat
     1561 ttgtctggct tgtcgaatag aggaggctat gcccaacctg tatacaagat tatcaagtct
     1621 tgactctaga atcttcacaa gagcgtggcc tgtctggtct tcgcttttct tagccttcac
     1681 aacatatttg acaaactgtt tttccatcac accgtagtaa gacctaagtc tttgtttctc
     1741 aagcagctgc tgtccatatt cagtcaattt tttgtctgct ctggatgtgc cctttacagc
     1801 cctatccata gccttcggat gtccataaac atttaaacca agtcttctgc tcagtttgaa
     1861 tctgggtcct ttcatattag ccattaatta tcatctccat aaagaaattt gccgcgataa
     1921 ttttttaagc caggaacatt ataacatata tggaggacca tgtaaatgat aatttatatc
     1981 atttacctca aggcatcctt tgcttccttg taagctattc ttgatttttc tattgcttca
     2041 atggcttcat cgattccagc ccaaccacta acaataactt ccttttcctc caaacgcttg
     2101 tactctgaga aaaaatgttc aaattccttc ttcatatggg ctcccatatc atccactgtt
     2161 tcaatctccg taaatcttgg gtcgtttctg ggaactgcta ttattttttc gtccttacct
     2221 ttctcatcct tcataaggaa catccctatg acccttgcat gtacaagaca tccagggaat
     2281 gttggattgg ctattagtac catgatatca atgggatccc catcctcagc cagtgtatcg
     2341 ggtatgtagc catagtcagt aggatagaac attggtgaaa aaagcgacct gtccaattca
     2401 aaaaccttga attccttgtt gtattcgtat ttgttactgc tgccctttgg aatttctata
     2461 aaagccttca caatataatc catacatatt cctcccttct gtcatttaat tatagcagga
     2521 tttgaaataa tttcatctgt tttctgtgaa attatcaaag ttatactatt cttcatcttc
     2581 taaattttgt aaatcatatt tgattaatca attcactttg actttcatta ctgatgtaaa
     2641 attggcatat aaatgttata attagatggt attaagcaaa atggtccggg aggggaaagt
     2701 gttgaaacta tatataagtg cagatattga aggtgtaaca ggtgtaaccc attgggatga
     2761 aacatcaaaa aacaagggtg actattcaca gtttgcagac cagatgactg ctgaagttag
     2821 agctgcatgc gagggagcag gtgagtatgc agaggtggag attgtagtta aggatgccca
     2881 cgaaagtgga agaaatattg atcacagcaa gcttcctcac tatacaaggt taatcagagg
     2941 ttgggacgaa ggactctatt ccatggtcca ggggctggat gactcctatg atggtcttgc
     3001 atttattggg taccactcag gtgcgggcat tgaggggagc cctctgtcac ataccttaac
     3061 tccggggatt ctatccatta gaatcaatgg acaggctgtg gacgaattca tgcttcatgc
     3121 ctacattgct gcctaccata aggtaccggt ggtttttcta tcaggtgatc aggctctatg
     3181 taaaagagta aaggagatca atcccaatat tgagactgta gcggtaaagg aaggtagggg
     3241 aagctctaca attagcattc acccagatag agccgtcgaa ctgatcaggt cagggattaa
     3301 gaggggcctt gcaatggaaa aaaacatgct gctgattcaa ctacctgaaa cattccaggt
     3361 tgaggttgag taccctagac attatgaagc aagaaggaac tccttctacc caggtgcatc
     3421 acttgtagac agtacaactg taagctttac atcaaacgac tatattgagg ttctaagatt
     3481 tatacatttc acggtttaac aacagggaga gaacatgacg acaataacaa ttgatgataa
     3541 aaaagttaga aaaaaaatac tttccatgac attgcccata actgctgaaa atatcctgca
     3601 aatgacagct ggtgtagtat caatggccat ggttgggaga attgactctg tggcagttgg
     3661 agcaattggt atgagcaata tcctatttcg gataatatgg gcagttctaa aaggtattgg
     3721 gactgggaac tcggtttttg ttgcccagag ctttggagcc ggtaaccatg gtaaattgaa
     3781 ggatacaact gaacaggcat tcatattggc cttggggata tctatgattt ttcagcagct
     3841 tatatactgg aatacagaaa gtctcattgg tattttcaac cccacaccag ccctcctggc
     3901 aggaggtacc atgtacctga ggatattatc ctggagcctg ccatttacag caattattct
     3961 tctaacggca ggtatacttc aagggatggg aaatgcaaag actcccatga taattgttgg
     4021 aattctaaac ggtgtcaata ttgtattcag ctatgtactg atatttggca agtttggatt
     4081 acctgagttg ggattgaggg gagcagcaat agcatataac atatcatatg gaattgctgc
     4141 attgatgggc cttattgtat tgtttggtaa aaacggaaca tttgcaaagc taggtggagg
     4201 cttcaaacta aggttcaaca ggacacagtc aattagactt attaggtttg cgataccaac
     4261 atcatttgaa atgagcttct ggcaatttgc ctcaattata atcacacgag ctgtattgac
     4321 ctatggtgag gttgcctatg cagcatacca gcttggtctg caggccgagt ccatatcata
     4381 tatgcccgct gctgggtttg gaattgcagc atcaacattt gtaggtcaat ctgtcggctc
     4441 aagggacagt gagatgggga aaacatacat gaaacacctt ataaggttat cacttgttgt
     4501 aacctcagtg gcgggaggat tgatgatagt attaccatct caaatcatga gggccttttc
     4561 aaatgatcct gaaattatag ctgtcggtgc agtttatttg ttcataatgg gcctcttgca
     4621 gatacctcag aatttggcag gactcctgaa tggtgcatta agaggagccg gctatcccaa
     4681 ggcttccatg atcaatgtgg gagtgggact ttggcttgtc agggtgcctc tggtgctatt
     4741 tatgacatat gtaatggatg caggaattga atggatctgg attatgatgg gtgtggatct
     4801 tttggtccgt tttatacttg cggtgtttac ctacagaagg aaagacatat tcaattcgga
     4861 aaaggaagcg gtgggaagta tttttctata ggaggatttt atggttttaa ggataaagaa
     4921 tgttgatata gtaacaattg accaagagaa tcgaataatc gaaaacagca atatatatat
     4981 tgaggatggc attatcacac atattggtga tgaagtcctg ggaatagagg ttaaggatac
     5041 aatcgatgga aggaacaagg cagccttgcc gggacttgta aatgcccaca ctcacatggg
     5101 gatgagtctg ttgagaaact atgcagatga tttacccctt caccagtggt tgactgagaa
     5161 aatatggccg gttgaa
//
LOCUS       DASRQF010000184         3284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_277320, whole genome shotgun sequence.
ACCESSION   DASRQF010000184 DASRQF010000000
VERSION     DASRQF010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277320"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..644
                     /locus_tag="VFD79_05020"
     CDS             48..644
                     /locus_tag="VFD79_05020"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HZH93444.1"
                     /translation="MTFKLPSLSYGYDALEPHIDARTMEIHYTKHHQGYVNNLNKAIE
                     NEKGLQDMTIQELMASLDKVPESIRTAVRNNGGGHLNHSLFWESMSANGGGEPTGRLM
                     EMIKSDIGSFEDFKDEFKKAATGRFGSGWAWLVMDGDKLKITSTPNQDNPISDGLKPI
                     LGLDVWEHAYYLKYQNLRGDYIDAWFNVVDWKKVEERL"
     gene            784..1164
                     /locus_tag="VFD79_05025"
     CDS             784..1164
                     /locus_tag="VFD79_05025"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HZH93445.1"
                     /translation="MKINPGRVLAGLGRKAKDYYNNPERLKELLSTANLVIRDNDQLS
                     GVIDDIGKLISLVRDYGKKEYRMVSKGTIITVIAGLIYLVNPVDIIPDFLPGGFLDDA
                     AVIGYILSTLRGEIEKYSDWKKNQ"
     gene            1181..2176
                     /locus_tag="VFD79_05030"
     CDS             1181..2176
                     /locus_tag="VFD79_05030"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HZH93446.1"
                     /translation="MKKVFICLMVLIILVPSISTGQIARPGSIKQFSAELVEEENPFF
                     VLRWKDEEETISLYNNEAYREDLYVQVNFYVRNEDSVIMREMNFKYIDLAREEGGFTR
                     IDLDLLRLGIVEEPVNVMFSSYNFRVRIGLAQRDILGSYTLFGSYSPTVSSGKIYPYN
                     YASPWALQELDKAYEYSLIPDCISSNMRDVITREEFAQVMVAYYESKTGQEIDPVGQY
                     FADTDNQEVLKAAALGIITGYEDGTYRPDNPITRQDIAVVIGRTLQKVDPTLSLSYSP
                     VAIENGISAYAYDSMMFLVHNGILKGDETNNLNPLEHTTREQAVLLTTRAYEYFQ"
     gene            2246..3145
                     /locus_tag="VFD79_05035"
     CDS             2246..3145
                     /locus_tag="VFD79_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZH93447.1"
                     /translation="MFEQNIGELAALATAFSWTLVGIFFGKASSRIGSLSVNFIKLVF
                     GFAFLTVTALITRGVPFPIDASLHNWTWLTISGIIGFFIGDFFMLKAYIEVGVRISLF
                     MMATSPPMAALLGYFLLGEKLSLSGVIGMMVTIMGIVIVVMSRDRGGQGVRMKYSPRG
                     LFYAFMGALGQATGLIFSKVGLLDYNTFAATQIRIIAGFISFVVFVTIMGRWGELKLA
                     IKDKEAIKYTLYGSFFGPFLGVSMSLVALRYTTAGIASTITSITPITIIPFSIFLFKE
                     KVKPSEIFGAFVSVAGVAILLLL"
BASE COUNT          993 a          607 c          809 g          875 t
ORIGIN      
        1 tatattaata gagaacatta gattaatata gataaagggg agattttatg actttcaaac
       61 taccgagttt aagctatggt tatgatgcac ttgagcctca cattgatgca aggacgatgg
      121 agatccatta caccaagcat caccagggct atgtaaacaa ccttaacaag gccattgaaa
      181 atgaaaaagg acttcaggac atgacaatcc aggagttgat ggcatccctt gacaaggtac
      241 cagagagcat caggacagca gttaggaaca atggtggagg ccacctaaac cacagcctct
      301 tctgggagag catgtcagca aatggcggtg gggagcctac aggacgcctg atggaaatga
      361 tcaagtccga cataggaagc tttgaggatt tcaaggatga attcaagaag gccgccacag
      421 gtagatttgg tagtggatgg gcatggcttg tgatggatgg agacaagctt aaaataacat
      481 ctacacctaa ccaggataat ccgatttcag atggactaaa gccaatactt ggactggatg
      541 tctgggagca tgcatactat ctgaaatacc agaaccttag aggcgactac attgatgcat
      601 ggttcaatgt ggttgactgg aaaaaggttg aggaaaggct ttaataagaa acaatagaga
      661 taccagctca caggagctgg tatctttcat gtgtggataa ttgattggat tgtgtgcaaa
      721 ttgctgctgt ggtataatgg gtagataaaa taactttatt gtaaatagaa agagatgatt
      781 ttcatgaaaa taaacccagg acgagtgctt gctggactgg gccgaaaggc aaaggattac
      841 tacaacaatc cagaaagact aaaggagctt ttaagcactg caaatttggt aatcagagat
      901 aacgaccagc tttcaggggt aattgatgac atcggaaagc tgatatctct tgtgagggac
      961 tatggaaaaa aggagtacag gatggtttcc aagggaacca ttattacagt tatagcagga
     1021 ctaatttact tggtcaatcc agttgatatt attcctgact tcctccctgg gggttttttg
     1081 gacgatgctg cagtcatagg gtatatattg agcacgctta ggggagaaat cgaaaaatat
     1141 agtgactgga agaaaaacca gtagattttt ggaggaagaa atgaaaaagg tttttatatg
     1201 cttaatggta cttataatac tagtaccgtc tatttcaacg ggccagatag cgagaccagg
     1261 ctcgattaaa cagttttccg cagagctggt tgaagaggaa aacccatttt ttgtacttag
     1321 atggaaagat gaggaggaaa caatatcact ttacaacaat gaggcctaca gggaagacct
     1381 gtatgttcag gttaacttct acgttcgaaa tgaagactct gtcataatga gagagatgaa
     1441 tttcaaatat attgatctgg ctagggaaga gggtggattt acaaggatag acctggacct
     1501 tttaagactt ggaattgttg aagaaccggt aaatgtaatg ttctcatcct ataatttcag
     1561 agtcaggatc ggacttgccc aaagggacat attgggaagc tatactttgt ttggcagcta
     1621 cagccccacg gtttcaagcg gaaagatcta cccttacaat tacgcttcac cctgggcatt
     1681 gcaggagctg gacaaggcat atgaatacag cctgataccg gactgtataa gcagcaatat
     1741 gcgtgatgtt ataacgaggg aagagtttgc ccaggttatg gttgcctact atgaaagcaa
     1801 gacaggccag gaaatagacc cagtaggaca gtattttgca gatactgaca accaagaggt
     1861 tcttaaggca gccgcccttg gcataataac gggctatgag gatggaacct acagaccgga
     1921 taatcccata acaagacagg acatagcagt ggtcattggc aggacccttc agaaggttga
     1981 cccgacctta agtctttcct attctcccgt tgccatagaa aatggtataa gcgcctacgc
     2041 ctacgatagc atgatgtttc tagtgcacaa tgggattcta aagggtgacg agaccaacaa
     2101 cctgaaccct cttgagcata ccacaaggga gcaggcggta ctgcttacaa caagagcata
     2161 cgagtatttc cagtaatcac caggtctgcc atcgggcagg cctttgaata tgccacatat
     2221 attggattga atgagaggac attaaatgtt tgagcaaaat attggagagc tggctgcatt
     2281 ggccactgct ttcagttgga cccttgttgg aattttcttt gggaaggcca gcagcagaat
     2341 tggaagcctt tcggtaaact tcattaagct tgtatttgga tttgctttcc ttacagtaac
     2401 tgcattgata acaaggggag tgccatttcc cattgacgcc tcgttacata attggacttg
     2461 gctcactata tcaggaataa ttggattctt tattggagat tttttcatgc tgaaggcata
     2521 catcgaagta ggtgtaagga tatcactctt tatgatggcc accagtccac ctatggcagc
     2581 tctactggga tacttcctgc taggggagaa gctgtccctt tctggtgtta ttggaatgat
     2641 ggtaacaatc atgggaatag tcattgttgt tatgagcagg gacaggggag gccaaggtgt
     2701 taggatgaag tattcgccta gaggcctttt ctatgccttc atgggcgcat tgggacaggc
     2761 caccgggctg atattcagca aggtgggact tttggactac aacacatttg cagcaaccca
     2821 aattaggatt atagcaggat ttataagctt tgtggtgttt gtgaccataa tgggaagatg
     2881 gggggagctt aagcttgcca taaaggacaa ggaggcaata aagtataccc tctatgggtc
     2941 attctttgga cccttcctcg gtgtaagcat gtcccttgtt gcccttaggt atacaactgc
     3001 tggaattgct tccacaatta cttcaatcac tccaataaca ataattccat tttcaatatt
     3061 tctattcaag gagaaggtca agccctcaga gatattcggg gcctttgttt ctgttgcagg
     3121 tgtggcaatc ctgctccttt tgtagcaaat aaagaggaga gttcatcaag aactctcctt
     3181 aattattttt tggcttggtg gattttatac ccattagtcc tccaaatatc ccaagaagta
     3241 ctgaaaggga tagggtgaag acgaccaggg tagtcatgtg gtct
//
LOCUS       DASRQF010000185         6580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_754164, whole genome shotgun sequence.
ACCESSION   DASRQF010000185 DASRQF010000000
VERSION     DASRQF010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6580
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754164"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..104)
                     /locus_tag="VFD79_05040"
     rRNA            complement(<1..104)
                     /locus_tag="VFD79_05040"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            458..1369
                     /locus_tag="VFD79_05045"
     CDS             458..1369
                     /locus_tag="VFD79_05045"
                     /inference="COORDINATES: protein motif:HMM:NF040786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HZH93448.1"
                     /translation="MDIRQLKTFIEVSKLGSFSKAAEKLFLTQPTVTNHVQSIEKELD
                     TVLLNRMSKGVTPTKAGDIVYQHAIDIINLYDSMKYKIDEHKGNIEGRLEIASSSVPR
                     RHLLPQLIYGFRKEHPLVTFSIIDVDSSKVVQNILNGYIDFGFVGAVFEEANLEYIGV
                     MDDSLVFVTPSNIYADKIEMSSISIDDIIRQPLILREEGSGTGKVLKDALEHKGVKPN
                     QLLVSAIVADIETIKRMISLGTGSSFLSYKDAFDQSGNKLVQGNVFKVDELSLNRKFY
                     LVFHKKRKLSPLGEAFKEFALTYASIS"
     gene            complement(1370..2023)
                     /locus_tag="VFD79_05050"
     CDS             complement(1370..2023)
                     /locus_tag="VFD79_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZH93449.1"
                     /translation="MKRKTIKIMLADAQLLCLHGLKHILDAQDYIKVVASASNQMDIL
                     DRGLTTNPDILIMDPQQKDNDGFETIRLLKERGFKARIILLLENICNERYMEATKINI
                     EGYVLKTASPEKLLYSIKEVNRGVQYIDEDVKRELQYSDECQKISRIENERVDSLSQR
                     EYDILKLVASGLSNKAIADQLFISEKTVKNHLTQIFKKLEVTDRVQATLFAIRSGIR"
     gene            complement(2004..3146)
                     /locus_tag="VFD79_05055"
     CDS             complement(2004..3146)
                     /locus_tag="VFD79_05055"
                     /inference="COORDINATES: protein motif:HMM:NF013346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HZH93450.1"
                     /translation="MINEFIKAALLIFMAEMGDKTQIIAMTFTTQYMVKHVLSGVALG
                     VLANHGIAIVLGAIIGSMISGEAVNFAAGGLFIIFGWNALRMDESEDVEKRLKLHPVL
                     AVALAFFIGELGDKTQLTAMALSAESEFPAIILAGTTTGMVATSGLGILVGSRIGGKI
                     PETGLKIVSSLVFLGFGLLKVADAINHYIESFAAVAVIFAIVALVEIILIRRTIIVAK
                     EAPEKLKVKRAAEQLYQMTKRINEELDGVCLGQENCGTCAGAGCLLGYIRIILSDARR
                     NKEYYLDADTKLESLLVRDYEKDKIEDALLLILQELVDFGWTDEPRFIMTRVRESLDI
                     LIFGRRIRNTATMEEYLREIMDIDYPLGKRYMNILQREAADEKKDN"
     gene            complement(3281..4810)
                     /gene="hutH"
                     /locus_tag="VFD79_05060"
     CDS             complement(3281..4810)
                     /gene="hutH"
                     /locus_tag="VFD79_05060"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004822064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HZH93451.1"
                     /translation="MSKVILDGNSLTLEDFIRIVRGGYKVELSNDAMAKVEKARNLVD
                     RFVEEEKVVYGITTGFGKFSDLVISSEEAKTLQRNLIISHSCGVGNPLHEEAAKGIMV
                     LRINALAKGFSGIRPSTLDTLVQMVNKGVNPVIPEKGSLGASGDLAPLAHMVLVMLGE
                     GEAFFNGERMPGAKAMEKAGIETVELTSKEGLALINGTQVMTSIGALAVYDAIMLSKT
                     ADIAASLTIEALNGITDAFDPKVHNARGHLGQINSADNLLKILKGSRMTTRQGEMRVQ
                     DPYSLRCTPQIHGATKDALEFIKSKVEIEMNAATDNPLIFTEEEEVISGGNFHGQPMA
                     LPFDFMGIAIAELANISERRLERLVNPALSNGLPAFLTRKGGVNSGFMIVQYSAAALV
                     SENKVLAHPASVDSIPSSANQEDHVSMGTIGARKAREILDNATKVIAMELMGACQAID
                     IRGKTGLGMGTEQAYKVIRDRIVTLEDDRIMYTDINIAEDIVQSWEIVSKVEAEVGEL
                     R"
     gene            complement(4980..5996)
                     /locus_tag="VFD79_05065"
     CDS             complement(4980..5996)
                     /locus_tag="VFD79_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZH93452.1"
                     /translation="MATTIKDVAKLAKVSISTVSRVINDSKPVSPEARRRVLKAIEEL
                     GYKPNEVARSLVTKKSNLIGIIVDDIGSSYVAQIIRGVEEVGRMYNYDILLCSSYGDK
                     EAEMRFVQLLMQKQVAGIIMVSEILNSEVLTYLDMSKIPFIYLNKYYNVLESPTVAIN
                     NREASDKVMKHLMDEGHRKIAYVTQEKDMELTIEKQKIEAYREWMASIGETPIVFEVD
                     GHNISDGYEVGVRIKDKLLEGNITAVYCCEDEIALGLINYFYDHNIRVPEDISVVGYG
                     DISLASYYRPKLTTIKEPYYDIGAVSIRRILKVLQGEKIEEQTLVLPVQLIERDSTSR
                     PKNI"
     gene            complement(6049..>6580)
                     /locus_tag="VFD79_05070"
     CDS             complement(6049..>6580)
                     /locus_tag="VFD79_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93453.1"
                     /translation="DKEKIENIKSKTHKLSMEVFGRNQKGIVSLMDIAILPKSQEGFE
                     TKLFRFLGAINESIGAELSSALIIENGEVIDQFTRDGVAGNWIENKSISKDMVSEVLN
                     TRESSLSVNWSEAVYNMGTVDITSLKSTILTPVLVGSSIKAIIYCESSLQRKDFSSAD
                     VMAVEVLSGVFSVNLV"
BASE COUNT         1732 a         1569 c         1151 g         2128 t
ORIGIN      
        1 ccaaaatcca tccgaaaatt tcattcgaaa tcacttcgct cgacttgcat gtgttaggca
       61 cgccgccagc gttcgtcctg agccaggatc aaactctcat taaaagtttg atgttagctc
      121 attatttgtt gtttaaaaaa attgaatctt tggtttattc aaagttctca ctgctgttta
      181 gttatctagg ttcatcgtct cctctgattt gaggtgacta gattattgta gcaaaaccag
      241 tcttgtttgt caatggtgtt ttgaatgttt ttcgtgactt tttacttgac tgtcttgact
      301 cttgtttcgc tcaacaaata ctaactatac agggtttcta attaaaagtc aatacgtttt
      361 tagagatttt tttcctttct ggcacaattt aattaaaggt tgatatttaa gggtttttca
      421 aatattatac aattaatata gaaaggagct gatcagggtg gatataagac aattaaagac
      481 atttatcgag gtttcaaagc taggcagctt ttcaaaggct gctgaaaaac tattcctaac
      541 tcagcctact gtaaccaatc atgtccaatc cattgaaaag gaactggata ctgtcctatt
      601 aaacagaatg tcaaaggggg tcacccctac aaaggcaggt gatattgtat accagcatgc
      661 cattgatatc ataaaccttt acgattcaat gaagtataaa atcgatgaac acaagggtaa
      721 catcgaaggc aggcttgaga tagcaagtag ctctgttccg agaagacacc tcttgcctca
      781 gttgatatac gggttccgta aggaacaccc actggtcaca ttctcaataa tagatgtaga
      841 ctccagcaag gtagttcaaa atatcctgaa tggatatatt gattttggct ttgttggtgc
      901 agtttttgag gaagcgaacc ttgagtacat tggagtcatg gatgactccc tggtatttgt
      961 aactccctcg aacatctatg cagacaagat agagatgagt agcatttcca tagatgacat
     1021 cataagacag ccacttatcc tgagggagga aggatcagga accggaaagg ttctaaagga
     1081 tgcacttgaa cataaggggg taaagcccaa ccagctcttg gtttccgcaa tagttgcaga
     1141 tatcgagaca attaagagga tgatttctct tggtaccggg tcttccttcc tttcatataa
     1201 ggatgcattt gatcagtcgg gaaataaatt ggtccaaggt aatgttttta aggtcgacga
     1261 actctctttg aataggaaat tctatctggt tttccacaaa aaaagaaagc tgtctccact
     1321 tggagaagcc ttcaaagaat ttgccttgac ctatgcttct atttcatagc tacctaatgc
     1381 ctgaacggat tgcgaaaagt gttgcctgaa cccgatcagt aacctcgagc tttttaaaaa
     1441 tctgagtaag atgattcttg actgttttct cacttatgaa cagctggtct gcaatagcct
     1501 tgttgctaag acctgatgcg accagcttaa gaatatcata ttccctctgg gaaagtgagt
     1561 ccaccctctc attctcaatc ctggatattt tttggcattc gtcagaatac tggagctccc
     1621 tcttaacatc ttcgtctata tactgcactc ccctgttcac ttcctttatt gagtagagta
     1681 gcttttcagg ggaagctgtc ttgagtacat atccttctat gttgatttta gtggcctcca
     1741 tatatctttc attgcatatg ttttcaagta gcaatatgat tcttgcctta aatcctcttt
     1801 ccttcaacag ccttatggtt tcgaatccat cattgtcctt ttgctgagga tccattatca
     1861 gtatgtctgg attggttgtc aatcctctat caagtatatc catctgattg ctggcgcttg
     1921 caacaacttt gatgtagtcc tgggcatcca gaatatgctt aagtccatga aggcacaata
     1981 attgggcatc tgccagcata attttaattg tctttctttt catctgcagc ctccctttga
     2041 agtatgttca tatatctttt acccagtgga taatcaatat ccataatttc ccttaaatac
     2101 tcttccattg tcgctgtgtt tctgatcctt cttccaaaaa ttaaaatatc caaagactcc
     2161 ctgaccctgg tcataatgaa ccttggttca tcagtccagc caaagtcaac aagctcctga
     2221 agaattagaa gcaaggcatc ctcaatcttg tctttttcat agtcccttac caggagactc
     2281 tccagcttgg tatcagcatc caaataatac tccttatttc ttcttgcatc ggagagtata
     2341 atccttatgt atccaagaag gcatcccgct cccgcacatg tcccacaatt ctcctgacct
     2401 agacaaacac cgtcaagctc ctcatttatc cttttggtca tttggtaaag ctgttcagcc
     2461 gctctcttga cctttagctt ttctggagct tctttagcca ctatgatagt ccttctaatg
     2521 agtattatct caaccaaggc aacaattgca aaaattacag ctacagctgc aaaactctca
     2581 atgtaatggt ttatggcatc agctaccttt aagagtccaa aacctaaaaa tactagtgaa
     2641 gaaactatct taagacctgt ttcagggatt tttccaccta ttcttgagcc tactagaatc
     2701 cccaatccac ttgtggctac catgccagta gttgttcccg ccaaaatgat tgctggaaat
     2761 tctgattccg cagatagtgc cattgctgtc agctgggtct tgtctccaag ctcaccgata
     2821 aagaatgcaa gggctaccgc caacactgga tgtagcttaa gccttttctc cacgtcctct
     2881 gactcatcca ttctcaatgc attccatcca aagattatga ataaaccacc tgcagcaaag
     2941 ttaaccgcct ctccactgat catgcttcct attatagcac caagtacgat cgctatccca
     3001 tgatttgcca aaacccccag ggctacaccg gatagaacgt gctttaccat atattgtgta
     3061 gtaaaagtca tggcaattat ttgagttttg tcccccattt cagccataaa tatcagcaac
     3121 gccgctttta tgaattcgtt tatcatccaa tacccaacct ttaaaaagac ttttagcata
     3181 tgctaaaagt cttttacatt ctacctatat attatacagt attcttatta cttagacaat
     3241 atcagtggaa tatatccttt ccacctgggt ctatacccat ttatctaagc tctcctactt
     3301 ctgcttccac cttggagact atctcccagg actgaacaat atcttctgca atattaatgt
     3361 cagtatacat tatcctatcg tcttccaaag tgactatcct gtctctgata accttgtacg
     3421 cctgttcagt tcccattcca aggccagtct ttcctcttat gtcaattgcc tggcaggctc
     3481 ccatcagttc cattgctatt acctttgttg cgttatcaag tatctctcta gcctttctag
     3541 caccaattgt tcccatggaa acatgatcct cctggtttgc tgatgaaggt atggaatcta
     3601 cacttgctgg atgggctaaa accttgtttt ccgataccaa tgccgctgct gagtattgaa
     3661 caatcataaa gcctgaattc actccacctt ttctggtcag gaaagctggc agaccattgc
     3721 ttagagctgg attcaccaat ctttcaagtc ttctttcgga tatgtttgcc aactctgcaa
     3781 tggcaatacc cataaagtcg aagggtagag ccattggctg tccgtggaag tttcctcctg
     3841 atattacctc ttcctcctct gtgaatataa gaggattgtc cgttgcagca ttcatttcaa
     3901 tctccacttt tgacttaatg aattccaatg cgtcctttgt tgcaccgtgt atttgaggag
     3961 tacatctcag tgaatagggg tcctgaaccc tcatttcacc ctgtctggtg gtcatcctgc
     4021 ttcccttcag gatttttaga aggttgtcgg ctgaatttat ctgtcccaga tgacctctag
     4081 cattatggac cttgggatca aaggcatcag tgattccgtt cagtgcctct attgttaggg
     4141 aagcagctat gtcggctgtt tttgatagca taattgcatc ataaaccgcc agtgcaccaa
     4201 tagatgtcat aacctgggtt ccgttgatca gggcaagacc ttcctttgat gtcagttcaa
     4261 ctgtctctat gcctgccttc tccatggcct ttgctccagg cattctttcg ccattgaaga
     4321 aagcttcgcc ttcaccaagc attaccaata ccatgtgggc caatggtgcc aaatcaccac
     4381 ttgcgcctag tgaccccttc tcagggatta caggatttac acccttgttt accatctgta
     4441 ccagtgtgtc cagtgtagat ggcctaattc cagaaaaacc cttggccaat gcattgattc
     4501 tcaataccat tattcctttt gccgcctctt cgtgcaaggg atttcccact ccacaggaat
     4561 ggctgattat aaggtttctc tgtaatgttt ttgcttcctc tgagcttatg acaaggtcac
     4621 tgaatttacc aaatccagtg gtgattccat ataccacctt ctcctcttcc acaaacctat
     4681 ccactaggtt tctggccttt tcaacctttg ccatagcatc atttgacaat tcaaccttat
     4741 agccacctct tacaattctt atgaaatctt ccaatgtcag actattgcca tccaatatta
     4801 ctttgctcat ataaatcctc ctcccactgg gctatactat ctagataaag tggctcaaaa
     4861 aaagccacag aataatctgt ggcttgattt ttttgaaatt tgtgaagcaa taatgtctta
     4921 cacatattat ccccacttct ctatttgttg aactaattat accatattca atcgaagtat
     4981 caaatatttt ttggtctgga tgtgctgtct ctttcaatca gctgtactgg aaggaccaat
     5041 gtctgttcct caatcttctc tccttgtagg accttcagaa tcctcctgat tgaaactgcc
     5101 cctatatcat agtatggttc cttgattgtt gtaagctttg gtctatagta ggatgccaag
     5161 cttatgtccc cgtaaccgac aactgaaata tcctccggta ccctaatgtt gtgatcgtag
     5221 aaatagttga tcagtcccaa tgctatttca tcctcacagc agtaaacagc ggttatattc
     5281 ccctccagga gcttgtcctt tattctgaca cctacctcat atccatccga tatgttatga
     5341 ccatccacct caaatacaat tggggtctca ccaattgaag ccatccactc cctgtaggct
     5401 tcaatcttct gtttctctat tgtaagctcc atgtcctttt cctgtgtaac gtatgctatc
     5461 ttcctgtgac cttcgtccat aagatgtttc ataaccttgt cactggcttc ccggttgttg
     5521 attgcaacgg ttgggctttc caatacatta tagtatttgt ttaagtaaat gaaggggatc
     5581 tttgacatgt ccaaatacgt caacacttct gaattcagta tctctgaaac cataattatt
     5641 ccagccacct gtttttgcat gagaagctgt acgaacctca tttctgcttc cttgtcccca
     5701 taactgctac aaagcagaat gtcatagttg tacattcttc caacctcttc tactccacgg
     5761 ataatctgtg ccacatagga gctgccaatg tcatccacaa taatcccgat caaatttgat
     5821 ttttttgtaa ccaggcttct tgccacctca tttggcttgt aacccaactc ctcaattgcc
     5881 ttgagcaccc ttctcctggc ttcagggcta actggcttgg agtcgtttat taccctggaa
     5941 acagttgata tggaaacctt ggcaagcttc gccacatcct tgattgtagt tgccatccta
     6001 tccctccttg aacgaccctt taatgaactg caggtcgcaa catatatatt atactaaatt
     6061 tacagaaaat actccactta aaacttctac agccatgaca tctgccgatg agaaatcctt
     6121 tctctgcagg ctggattcgc aatagataat cgccttgata gagcttccta ccaggacagg
     6181 tgttagtatt gtagacttaa gtgatgtaat gtcaacagtc cccatgttat aaacagcttc
     6241 actccagttt acggacaagg atgattccct ggtatttagg acttcactta ccatatcttt
     6301 ggaaatggac ttgttctcaa tccaatttcc tgcaacacca tctctagtga actggtctat
     6361 aacctctcca ttttcaataa tcagagccga actcagttca gcacctatgc tctcattgat
     6421 ggcccctagg aatctgaaca gctttgtctc aaatccttcc tgggactttg gcagtattgc
     6481 aatatccatt agggatacaa ttcctttctg gtttcttccg aacacttcca tggaaagctt
     6541 gtgggtcttt gacttgatgt tctcgatttt ctccttatca
//
LOCUS       DASRQF010000186         2989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_892362, whole genome shotgun sequence.
ACCESSION   DASRQF010000186 DASRQF010000000
VERSION     DASRQF010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892362"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1211
                     /locus_tag="VFD79_05075"
     CDS             <1..1211
                     /locus_tag="VFD79_05075"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZH93454.1"
                     /translation="GCAQTGTGKTAAFAIPILQGIHAEKQSAKGNRQIKALIVAPTRE
                     LAIQISESFKSYGRNLGLRTLVIYGGVGQNPQTKELRQGVDILVATPGRLLDLINQGF
                     VSLKTIKYFVLDEADMMLDMGMLKDVRKIIGFIPKVRQTMFFSATMPTEIGELAKSIL
                     NDPVRIDITPVSSTVEIIEQEVYSVDKVNKTNLLIHILKDKEIDSALVFSRTKHGANK
                     IAASLTKAGFTTEAIHGNKSQTARQLALSNFKKRKTRILIATDIAARGIDIDELSHVI
                     NYDLPEVPETYVHRIGRTGRAGLGGRAISFCDYGEKPLLRSIQKLTSRTIEEVKDHPY
                     PLVDTTIKPKEKSNRRPSSSRPSASKDLKQKGKEAKNGKVRREKRANDSKKANAKSNE
                     KRKNLHWSKG"
     gene            1391..2083
                     /locus_tag="VFD79_05080"
     CDS             1391..2083
                     /locus_tag="VFD79_05080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HZH93455.1"
                     /translation="MKKMGLLGGMSWESSLEYYRIMNETVKEKLGGSHSANCLMYSFD
                     FQEIEDLQHRGEWEKLKRLMIDEAKNLKKAGAESIVICTNTMHLMAPDIESGTGLKVI
                     HIAEATGDEILKRGVKKVLLLGTKFTMEGTFYREKLEEKGIEVIIPTESDRQVVHDII
                     YNELILGELNPQSRRKYVEIINRAADIGVTGVVLGCTEIPLLIKDKDVDIEVFDTTEI
                     HAKAAIEHAING"
     gene            complement(2120..2836)
                     /locus_tag="VFD79_05085"
     CDS             complement(2120..2836)
                     /locus_tag="VFD79_05085"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HZH93456.1"
                     /translation="MGKVRHFLSIVLLSTLILFIPRAAGYFASLFDYSSIDPDGSFAW
                     ISVHHIFQAFVFLLIMVLLKKKYHFDFGFHWGDKATGKLITLRFIKYFTLYNIGAYAI
                     LILTNSFQVFQFPLKSINIIGYMGFQLLLSGPSEELIFRAFAITMFTMLIKGKPIANH
                     ISPATLLAAVIFAIAHIGFSFAPFAVTYNASQLLLALVLGIFYGICYEKSGSMYYPMI
                     MHSYSNILMVGTTIVLSLVQ"
BASE COUNT          993 a          518 c          725 g          753 t
ORIGIN      
        1 tgggttgtgc tcaaacgggt acgggtaaaa ctgcagcttt tgcaataccc atactacagg
       61 gaatacacgc agaaaaacag tcagccaaag ggaatagaca gataaaggcg cttatagttg
      121 cacccacaag ggagctggct atccagatat ccgagagctt caaatcctac gggaggaatc
      181 ttggattgag gaccttggta atatatggag gtgtgggcca gaacccgcaa acaaaggaat
      241 tgcgccaagg tgtggacatt cttgtagcca caccaggaag attgctggat ctcatcaacc
      301 agggctttgt gagcctaaaa accatcaagt actttgtcct cgacgaggct gacatgatgc
      361 tggatatggg tatgctaaag gatgttagga agataatcgg atttattccc aaggttaggc
      421 agaccatgtt cttttcagcg accatgccga ctgaaatagg tgaattggct aaatccatac
      481 tgaatgatcc agttagaatt gatatcacac cggtatcatc tacagttgaa ataattgagc
      541 aagaggtata ttctgttgac aaggtcaata agaccaattt gcttattcac atactgaagg
      601 acaaggaaat tgattctgca ttggtatttt ctcgaacaaa gcacggtgcc aacaaaatag
      661 ctgcatccct tacaaaagca gggtttacaa ctgaagccat acacggaaac aagtctcaaa
      721 ccgccagaca attggcattg agcaatttca aaaagagaaa aacaaggatt cttattgcaa
      781 cggatattgc tgcaagaggt attgacatcg acgagttgtc acatgttata aactatgacc
      841 tgcctgaggt gcctgaaacc tacgtccaca ggattggaag gactggaagg gcaggcttgg
      901 ggggcagggc catatcattc tgcgattatg gtgaaaaacc cttgctcagg tcaatccaga
      961 agctgacatc caggaccata gaggaagtaa aggaccaccc ttatccccta gtggatacga
     1021 ctatcaagcc caaggaaaag tcaaacagga gaccatcatc atccagaccg tctgcatcaa
     1081 aagaccttaa gcagaagggc aaggaagcaa agaacggcaa ggtaagaaga gagaaaaggg
     1141 caaatgattc aaaaaaagcc aatgcaaaga gcaatgagaa gagaaagaat cttcactgga
     1201 gtaagggcta actgaatgga tagacaaaga aaattcttga gtaatcagga gttttctttt
     1261 ttttttgaaa tgcatattag tttgctggat tgtttaaata ctgaggtttt acttgcagat
     1321 gttacgtgta tatcgtaaat ccttgttttg agggtatata gaaaaggata taaaaaagga
     1381 gggagcaatt atgaagaaga tgggattgtt gggtggaatg agttgggagt cttcacttga
     1441 atactatagg ataatgaatg aaacagtgaa ggaaaagttg ggaggttccc attcagccaa
     1501 ttgtttgatg tattcctttg acttccaaga aattgaggat ctgcagcata ggggagaatg
     1561 ggaaaaacta aaaagattga tgattgatga agccaagaac ctgaagaagg ctggtgctga
     1621 atctatagtg atatgcacga atacaatgca cttgatggcg cctgatatag agtcgggtac
     1681 tggtctaaag gtgattcaca tagcggaagc cacaggtgat gaaatcctaa agaggggtgt
     1741 gaagaaggtt ctcctgcttg ggaccaaatt tacaatggaa ggaaccttct accgggaaaa
     1801 gcttgaggag aaagggattg aggtgataat cccgactgaa tcagacagac aggttgtcca
     1861 tgatatcatc tacaacgagc tgatacttgg tgaattgaat ccacaaagca gacgcaagta
     1921 tgttgagata atcaataggg ctgctgacat tggtgtaaca ggtgtagttt tggggtgtac
     1981 agagattcca ctgcttatca aggacaagga tgtggacatt gaggtatttg acaccactga
     2041 gatccatgct aaggcagcca ttgagcatgc aattaatgga taatcaagta aatgcaggtg
     2101 ttgaaggggg atgccggttt cattgaacaa gggacaacac tatggttgtt ccaaccatca
     2161 atatgttgct atagctatgc attatcatgg ggtaatacat gcttccgctt ttttcatagc
     2221 agattccata gaaaatgcca agtacaagtg ccagaagcaa ttgtgatgca ttgtatgtta
     2281 ctgcaaaagg tgcaaaggag aagcctatgt gggctattgc gaaaatcacc gctgcaagaa
     2341 gggttgcagg actgatatgg tttgcaattg gttttccttt gattagcata gtaaacattg
     2401 tgatggcaaa agctctgaaa atcaactctt ctgaaggacc agagagcaga agctgaaatc
     2461 ccatgtaccc gataatattt attgatttta aagggaattg aaaaacctgg aagctgttgg
     2521 ttaagattaa tatagcatag gctccaatgt tgtacagggt aaaatactta atgaatctaa
     2581 gggttatgag tttacctgtt gctttgtctc cccaatgaaa accaaaatca aaatggtatt
     2641 ttttcttaag taagaccatg atcaacagaa aaacaaatgc ctgaaagata tgatgcacag
     2701 aaatccaagc aaaggaccca tcaggatcaa tggaggagta gtcaaacagg ctggcaaaat
     2761 agcccgcagc ccttgggata aagagaatca gagttgataa taggactata gataagaaat
     2821 gtctaacttt tcccatgaat atctccttgt atttttgata ttcaccatta gttcacttca
     2881 taagctcctg gtaattgttc tttgtaaccg gctgattgta gattaaaaat ccaggctcca
     2941 cattagttga gaaatgtgac atggtgaaat gcagatgtgg acctgtgga
//
LOCUS       DASRQF010000187         2231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_513789, whole genome shotgun sequence.
ACCESSION   DASRQF010000187 DASRQF010000000
VERSION     DASRQF010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513789"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..659
                     /locus_tag="VFD79_05090"
     CDS             201..659
                     /locus_tag="VFD79_05090"
                     /inference="COORDINATES: protein motif:HMM:NF024076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HZH93457.1"
                     /translation="MKSSKKQLLRNQDIYPSSDAIEKALGNSNKAYIKFVNELSNHDI
                     HLEWSYYNDGKAWLAKGLFKWTGIRGGKNEKTVFWLSIWDGFFKVTMYFPEKARVDVL
                     SLPLDKELKLAIEVSKQMGKLKYFPIIFDLYSDEMFEAVFLLADLSKSLK"
     gene            753..2147
                     /locus_tag="VFD79_05095"
     CDS             753..2147
                     /locus_tag="VFD79_05095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HZH93458.1"
                     /translation="MWENDNWNKQVDVLVVGTGFAGLSAAIEAGEAGKRVLVIEKMKT
                     AGGNSIISDGGIAAPGTQLQEKHGIEDSQQLMYDDMLRAGLGLNHPDLLRIFVSNAQD
                     AFQWTIDHLNVPYMDRVDLFGGHSLPRCYTPEGITGNTILRKMLKRIGELNIPILYKT
                     CFRRIITDDSGNVSGCKVDAEYDYKSNTGVSLYIKTNRGIILASGGYGADVGFRSIQD
                     PRLDKGIQTTNKPFATAEVLKEALRIGAASVQLSHIQLGAWTSPDEPGFGHGPMFADY
                     IVFKDGVILDPKSGKRIVNELGDRKQSADAILTAGHPCLGIADHRAVVDSGWDISKAI
                     EKEVVRVFDTIEDLASFYGIDNKSLYEFIDCYNGFVRKGKDEDFGKPILQNAKEIENP
                     PYYAMRLWPKVHFTMGGLRINDKAQVVDFDGNVIKRLYAAGEVAGGVHGASRLASCAI
                     TECIVFGRLAGQVI"
BASE COUNT          705 a          388 c          576 g          562 t
ORIGIN      
        1 actattggaa aagaacatgc tgccggactt cattataaga tggggtgctc gtcgtttaac
       61 tgggaggcgg ttaaagcaaa ttacatctca gagtgttgag ggacatcgtg ccaactttat
      121 ggaatatgtt gaggacctaa gaacaagaca gagggacagg aacactgtct tatagataat
      181 aaaagttgga gactgaatcc atgaaatcta gcaagaagca attacttaga aatcaagata
      241 tatatccgtc aagtgatgca atagaaaagg cgcttgggaa ttctaacaag gcctatatca
      301 agtttgtcaa tgaactttca aaccatgata tccacttgga atggagttac tacaacgatg
      361 ggaaggcatg gctggcaaaa gggcttttta agtggacagg catccgaggt ggtaagaatg
      421 aaaaaactgt tttttggcta tcgatctggg acggtttttt caaggtgacc atgtattttc
      481 ctgaaaaagc gcgtgtagat gtattgagtc ttccccttga caaagagctc aaactagcga
      541 tagaagtttc aaaacaaatg ggaaagttga aatacttccc aataattttc gacctgtatt
      601 cggatgaaat gttcgaggca gttttcttgc ttgctgattt aagtaaaagc ttaaaataga
      661 cgattgaaca aagaggaatc tgaaaatgct gtaaagtaga ttaaatctta aataacaata
      721 aaatcttgga atcaatcatc aggaggtgta gtatgtggga aaacgataat tggaacaaac
      781 aggttgatgt tcttgtggtg ggaacaggct ttgccggctt gtctgctgct attgaggctg
      841 gtgaagctgg aaagagggtc ctggtaattg agaaaatgaa gacggctgga ggaaactcaa
      901 tcatcagtga cggggggatt gccgcacctg gaacacaact gcaggaaaag catggaattg
      961 aagatagcca gcagctaatg tatgatgaca tgctaagggc aggacttgga ctaaaccatc
     1021 cagacctgct gcgtatcttt gtatccaatg cacaggacgc ctttcaatgg accatagacc
     1081 atcttaatgt gccatatatg gatcgggtag acctctttgg tggtcacagc cttccaaggt
     1141 gctacactcc agaggggata acaggaaata caatccttag aaagatgttg aaaagaatag
     1201 gtgaactgaa tattcccata ctctacaaga cctgcttcag aaggattatt actgatgaca
     1261 gtggtaatgt tagtggctgc aaggtagatg ctgagtatga ctataagtcc aacacaggag
     1321 tatccttata cataaagacc aataggggga taatattagc atcaggtggc tatggggctg
     1381 atgttgggtt cagatcaatc caggacccaa gactggataa aggcattcaa accaccaata
     1441 agccctttgc aacggcagag gtcttgaagg aagctttaag aataggtgca gcatctgtcc
     1501 agctatcaca tatacagttg ggggcctgga cttcgcctga tgaaccgggc tttggccacg
     1561 gtccaatgtt tgccgattat attgtattta aggatggagt catcctggac ccaaaatcag
     1621 gaaaaagaat tgtcaatgag cttggtgaca ggaaacagtc agctgatgca attttgacag
     1681 caggccatcc ttgcctggga atagctgacc acagggcagt tgttgactca ggctgggaca
     1741 tttccaaggc catagagaaa gaggttgtca gggtctttga tacaattgaa gacctggcat
     1801 ccttctacgg aattgacaac aagtcattat atgaatttat tgattgctac aacggctttg
     1861 taaggaaggg taaagatgag gactttggca agccaatatt acagaatgcc aaagagattg
     1921 agaatcctcc atactatgca atgaggctat ggcctaaggt tcatttcaca atgggtggcc
     1981 ttaggataaa tgacaaagct caggtggtgg attttgacgg caatgtcatc aagagacttt
     2041 atgctgcagg tgaggttgct gggggtgtgc acggagccag tcgtctggca agttgcgcaa
     2101 taacagaatg cattgtattt ggaaggctcg ctgggcaggt aatttgagtc aaaaagaacc
     2161 gtccccaggg actcaatagg gaggtaagct atgggaaagt atgacaaatc agtagaagag
     2221 atgtggaaga a
//
LOCUS       DASRQF010000188         4368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1492350, whole genome shotgun sequence.
ACCESSION   DASRQF010000188 DASRQF010000000
VERSION     DASRQF010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492350"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="VFD79_05100"
     CDS             <1..1157
                     /locus_tag="VFD79_05100"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZH93459.1"
                     /translation="ITANFHHPVNPTYFTMLDLPSHVFGTSFAMMVFASFLTSPLWGS
                     WGDNKSRKMTLVYATILYGSAQIVYGLANNIWQILAIRAIAGLFSGGFMVGLMAMIVD
                     VSSVDKRGERIAAYSALMSVSMAVGFLTGGALGYLPIRYVFFIQGGVMILIGLSIYFF
                     MEESNSPLRKGSKPQFIWNILGEKEKREAVFTPWILLFLGITFFAFVAFSSNNNAFNY
                     YLRDELNFEPIVNGIWKATTGIIGLVANLTINVWIVRKTELKTSMRILLGLALMSATM
                     IYLKDSTYPFMIWSLFYFTFHTILFPLLQNFAVEDSQHGAGFMSGIFNAVKALGEMSG
                     AIIAGFAYGLGSKVPFLLAAAALLIALLLGFVQSMGKNEAGILARGDKNI"
     gene            1429..3768
                     /locus_tag="VFD79_05105"
     CDS             1429..3768
                     /locus_tag="VFD79_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZH93460.1"
                     /translation="MKDKFRLRAEELTSICDQEDLGFETTEDMSPLKGIIGQDRGTQA
                     LGFGLKIKKKGYNIYVSGLSGTGRSTFTYSIAEGYAKQEPVPLDWVYVYNFNRKESPK
                     ALSFMPGKGKEFKKELEGFIDHLKRTIPETFAGIEYETRKNDVIKIFNQKKGNVLKKL
                     NEKSAEYGFVYTPSEQGLVSIPMKDGKPINEEEYAALTMEEREDMMEKYEELRIATVE
                     EFNDLRILDEELSESINQLDQNVVLEIIRYDIKKLMDKYGQRPATAIYLNELEKDILE
                     NLGKFKTKNAPKNFSLFDIPTGNDENFFIRYKVNLFVDNSQLENAPIILESNPIYNNL
                     MGHIEYKSQMGVLTTDFTQIKPGSLHQANGGYIIFQMKEILNNPISWEMLKRSMKTNE
                     INIENYNRLMGMAVTATLKPEPIPLDVKVIIIGDDYTYNILYNYDDDFRKLFKVMADF
                     DIEMDKNVENLKRMGEFIATHCQESNIRHFDRAAVSRVVEYSTRIAGHQDKLSTQFNQ
                     IIEILYEADFWATEEGSDHVNERHVKKAIDEKIYRSNKYEQKLNEMFMEGTLLIDIDG
                     EKVGQINGLAVMGTGEYSFGKPSRITATVYSGEEGVINIEREAKQSGRIHDKGVLILS
                     GYLGQRYARKKPLGISIGLGFEQSYSVVDGDSASSTELYAILSSMADAPIKQYLAVTG
                     SVNQKGEIQPIGGVNEKIEGFYQVCRLIGLTGNQGVVIPRSNIKNLVLRDEVIQAVEK
                     GDFHIYAIDNVEEGVQILMDRTMEDLDQVINRKLEEMSK"
     gene            3814..>4368
                     /locus_tag="VFD79_05110"
     CDS             3814..>4368
                     /locus_tag="VFD79_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZH93461.1"
                     /translation="MTYNFDEVIDRSGTYSSKWDEEVQMDLFGKSGLLPYWVADMEFK
                     AAPEILDALGKRVQHGIFGYTARPPQYYKGVIDWTRKRFAWDIERNWILFTPGIVPGV
                     NYTIQALTEEGDGVIINEPVYYPFSRSIEENDRVVVNSPLVLKDKSYHIDFEDLEEKA
                     KDPKNKLYVLCSPHNPVSRVWSPEE"
BASE COUNT         1381 a          840 c         1088 g         1059 t
ORIGIN      
        1 tcataactgc aaactttcat catccggtta acccaaccta ctttacaatg ctggaccttc
       61 ccagccacgt tttcgggaca tcctttgcaa tgatggtctt tgcatccttc ctgacatctc
      121 ctctttgggg aagctggggg gacaacaaga gcaggaagat gaccctggta tatgcaacca
      181 tcctatacgg atccgcacag atagtatacg gactggccaa caacatctgg cagatcctcg
      241 cgatcagagc cattgccggc ctgttttcag gaggcttcat ggtagggctg atggccatga
      301 tagtggatgt aagcagtgtg gacaagagag gagaaaggat agccgcctat tccgccctta
      361 tgagcgtttc aatggcagta ggctttctta cgggaggagc cttgggctat cttccgatca
      421 gatatgtatt ctttatacag ggcggtgtga tgatacttat aggactttcc atatatttct
      481 ttatggagga atcaaatagc cctttgagga aggggtcaaa gccccagttc atctggaaca
      541 tactcggtga gaaggagaag cgggaagctg tctttacacc atggatcctc ttatttttgg
      601 ggatcacttt ctttgccttt gtggcatttt caagcaataa taacgcattc aattactatc
      661 tcagggatga attgaacttt gagcccatag taaatggaat ctggaaggct acaaccggaa
      721 taataggcct tgtggcaaat ctaaccatca atgtgtggat agtaagaaaa acagagctga
      781 agacatccat gaggatactc cttggacttg ccctcatgtc tgcaaccatg atatacctta
      841 aggattcaac atatccattt atgatttgga gtcttttcta cttcacattt catacaattc
      901 tatttcctct actccagaat tttgcagttg aggactccca gcacggtgcc ggattcatgt
      961 ctggaatatt caatgcagta aaggcactgg gggaaatgtc tggggcaatc attgcaggct
     1021 ttgcttatgg acttggaagc aaggttccct ttctcctggc agcagcagcc cttcttatag
     1081 ccttgctatt gggttttgtt cagagcatgg gaaagaatga agccggaata cttgcaaggg
     1141 gagacaaaaa catataaaat ggagtcaaag gtaaaacatt ggaggaatac aaaatgcatc
     1201 taagagacaa gaagaagata acaatggtca tagtgattgt aatagcattt gcaatgattg
     1261 caccactttt gctgaatgcc ctatattaca tctagaaatt tcatgaaaga ttatcagtga
     1321 gccaggccgg ggggtctggt tttttcttta tataagctta attattggta ttacagattt
     1381 tcatgggtat atacaatgta agtgaaactc tagcagggag ggatgatagt gaaggacaag
     1441 tttagattga gggcagaaga gcttacgagc atttgcgacc aggaagacct tggctttgag
     1501 accacagagg acatgagccc acttaagggt ataataggcc aggacagggg aacccaggca
     1561 cttggatttg gtctgaaaat caaaaagaag gggtacaata tttacgtgtc tggcttaagt
     1621 ggaactggaa gaagcacatt cacctattcc atagcagaag ggtatgctaa gcaggagccg
     1681 gtcccactgg actgggtgta tgtatataac ttcaacagga aggagtctcc aaaggcactt
     1741 tcttttatgc ctggaaaagg caaggaattc aaaaaggagc ttgagggatt tattgaccat
     1801 cttaaaagga caatacctga aacctttgca ggtattgaat atgaaacaag aaaaaatgat
     1861 gtcataaaga tattcaacca gaagaagggg aatgtcctta aaaagcttaa tgagaagtca
     1921 gctgagtatg gctttgtata tacaccaagt gagcaggggc tggtttcaat tcccatgaag
     1981 gatggcaagc ccataaatga agaggaatac gcagccctta caatggaaga acgggaagac
     2041 atgatggaga agtacgagga gcttaggatt gcaacagttg aggagttcaa cgacctgagg
     2101 atccttgatg aggagctttc agagagcata aaccagttgg accagaatgt ggtccttgaa
     2161 atcataaggt atgatatcaa gaagctaatg gacaagtatg gccagaggcc agccacggca
     2221 atctacctga atgaactgga aaaggatatt cttgagaacc tcgggaagtt caagaccaag
     2281 aatgcaccga aaaacttctc cctgtttgat attccaacag ggaatgacga aaactttttt
     2341 ataagataca aggtgaatct gtttgttgac aacagccagc ttgaaaatgc cccaataatc
     2401 cttgaatcga accccatcta caacaacttg atgggtcata ttgaatacaa gagtcagatg
     2461 ggtgttttga caacagactt cactcagata aagccaggat cactccacca ggcaaacggt
     2521 ggatatataa tattccagat gaaggaaatc ctaaacaatc caatatcctg ggaaatgctg
     2581 aaaaggtcca tgaagaccaa cgaaataaac attgagaact acaacaggct tatgggcatg
     2641 gctgtcacag caacactcaa acctgaacca atacccctgg atgttaaggt aataataatc
     2701 ggtgatgact acacttacaa catcctctac aactatgacg atgacttcag aaagctcttc
     2761 aaggtaatgg ccgactttga tatcgagatg gacaaaaatg tagagaacct taagaggatg
     2821 ggtgagttca ttgcaaccca ttgtcaggaa agcaatatca ggcacttcga cagggcagcc
     2881 gtttcaaggg tggttgaata cagcacaaga attgctggac accaggacaa attgagcacc
     2941 cagttcaacc agataattga aatactctat gaagctgact tctgggctac tgaggagggc
     3001 tctgaccatg tcaatgaaag acatgtcaag aaggcaatag atgaaaagat atacaggtcc
     3061 aacaagtatg aacagaagct gaatgagatg tttatggaag gaactctcct gattgatatt
     3121 gacggggaga aggtagggca gataaatggt ttggcagtta tgggaaccgg cgagtacagc
     3181 tttggaaagc ccagcaggat aaccgccact gtctacagtg gagaggaagg cgtgatcaac
     3241 atagagaggg aagccaaaca aagtggcagg atccatgaca agggcgtcct tatactgagc
     3301 ggatacctgg gacagaggta tgcaaggaaa aagcctcttg gaataagcat aggcctgggc
     3361 tttgagcaga gctactcagt tgttgacggt gacagcgctt caagcacgga gctttatgca
     3421 attctctcat ccatggcgga tgcaccaatt aagcagtact tggcagttac aggttctgtg
     3481 aaccagaagg gtgaaatcca gccaataggt ggggtcaacg aaaaaataga gggcttctac
     3541 caggtttgca gacttatagg cttgaccggg aaccagggag tggttatacc caggtccaac
     3601 atcaagaacc ttgtactaag ggatgaagtc atccaggcag tggaaaaagg tgatttccat
     3661 atctatgcaa tagacaatgt tgaagagggg gttcagattt taatggatag gaccatggag
     3721 gacctggacc aggtaatcaa caggaagctt gaggaaatga gcaaatagtg aaaagtgaga
     3781 taatctcata aaataatata caggaggaaa gaaatgactt ataattttga tgaagttatc
     3841 gacagaagcg gcacctattc ttcaaagtgg gatgaggagg tccaaatgga ccttttcggt
     3901 aaatccggac tgcttccata ctgggtggcg gacatggagt tcaaggctgc tccagagata
     3961 ctggatgcac tgggaaagag ggtccagcat ggaattttcg gctatactgc aagaccaccc
     4021 cagtattaca agggagtgat cgattggacc aggaaaagat ttgcttggga tattgagagg
     4081 aactggatcc tatttactcc cggcatagtc cctggggtta actacaccat tcaggcactt
     4141 actgaggaag gtgatggtgt aataatcaac gagccagtct actatccatt ctcaaggagc
     4201 atagaggaga atgatagggt agttgtaaac tcacccctgg ttctaaagga caagagctac
     4261 cacatagatt ttgaggacct tgaagagaag gctaaagatc ccaaaaacaa gctgtatgtg
     4321 ctttgcagtc cccataatcc cgtatccagg gtatggagtc ctgaggag
//
LOCUS       DASRQF010000189         3652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1754882, whole genome shotgun sequence.
ACCESSION   DASRQF010000189 DASRQF010000000
VERSION     DASRQF010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1754882"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VFD79_05115"
     CDS             <1..775
                     /locus_tag="VFD79_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZH93462.1"
                     /translation="EEKIKQELARIVEMNDHEGFYLHYQPIIDIRDNTVSGFEALARM
                     QTQDLGFVSPLEFIPIAEKTKLIIPLGEMIIVKAFEFLKKIKERGNKRVKISINISVI
                     QLLREGFVEGVFSIMDKMEISPEDVILELTESVFGINNDDLNEILASLRDAGVQIAID
                     DFGTGYSSLARERELSVSCLKINKSFIDRLMFLEPERTITRDIISMGHKLGHYVVAEG
                     VEYQEQRDYLERCGCDMLQGYLISRPLDGESAIRFIEEY"
     gene            873..1430
                     /locus_tag="VFD79_05120"
     CDS             873..1430
                     /locus_tag="VFD79_05120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZH93463.1"
                     /translation="MELSKDVLLKEIQDLRDENKRLRKQLEEMNPRDRLTGLLNRGSL
                     YERLEYEAVRTDRGQSYLSLLLIRISDFKGLVQEHGKDTMDVLVKLMGQAISESVRSV
                     DIVGRYEDGGFLLLLPDIQPNKGTVVSERLKKAIDRQISPMGIRIKYSIGVKLYRNEP
                     INELLTETERLAMSSEAEGIGRVHM"
     gene            complement(1492..2502)
                     /locus_tag="VFD79_05125"
     CDS             complement(1492..2502)
                     /locus_tag="VFD79_05125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HZH93464.1"
                     /translation="MMRKILGLILALMIVIQPFTAEAAAFGDVSSTDWFHDNVNLLVR
                     EGAIQGYPDNTFRPKNTMTRAEFIKSTMSILGYKDIPSKGTHWADGYIEKAKTLGIVC
                     NDTSSNPDSPITRYDMARIVSNVLDYQNYRPQADLAQYEQLIKDIRQVSSLGNPEIKT
                     AVLNSYSSGILTGYVDGAFRGDATLTRSEAATVLLRIKYQQYRSLPRVELNSNFEQRV
                     LDLVNVERGKEGLSPLRMHSELSKVAYEKSKDMAIYDYFDHTSPNYGSPFQMMKEFGI
                     KYNYAGENIAMGHPTPEAVVKAWMESPGHRANIMNMNFGNLGVGIYNDNGTIYWTQMF
                     IN"
     regulatory      complement(2622..2695)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            3097..>3652
                     /locus_tag="VFD79_05130"
     CDS             3097..>3652
                     /locus_tag="VFD79_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009430573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZH93465.1"
                     /translation="MYLNCYDEVKNITRDLVKILSIVRQGGESDVAKWIYDYYMDLDY
                     FRNNPDQVVFQETLNDEVLRHNAIALVKGTKGVSNRTVVMMGHIDTVGVDDFGTNWKA
                     AFDPDRLPEVLKEMGVSEEVIEDIESGEYMFGRGALDMKAGVAGHMYLIKYFAENPHE
                     LDGNLIAIAECDEEDNSHGIISALK"
BASE COUNT         1053 a          708 c          873 g         1018 t
ORIGIN      
        1 tgaggaaaag atcaagcaag agctggccag gatagtggaa atgaatgatc atgaaggatt
       61 ctatctccat taccaaccca ttatcgatat cagagacaac actgtaagcg gctttgaagc
      121 cttggcaaga atgcaaaccc aagaccttgg atttgtatct cctcttgagt tcataccaat
      181 agctgaaaaa acaaagctga ttatccccct tggtgaaatg ataatagtca aagcctttga
      241 gtttttaaag aagatcaagg aaagaggtaa taaaagggtt aagatttcaa ttaacatttc
      301 tgtaatacag cttctaaggg aaggctttgt ggagggtgta ttcagtatca tggacaaaat
      361 ggagatttca ccggaagatg taatacttga gttgactgaa tctgtgtttg gaattaataa
      421 tgatgacctt aacgagattc ttgctagctt aagggatgct ggagtacaaa ttgcaattga
      481 cgatttcggt acaggatatt cctcccttgc aagggagagg gagcttagcg taagctgcct
      541 taagataaac aagtccttta tagacaggct tatgtttctt gaacctgaaa ggacaatcac
      601 aagggatatc atttccatgg ggcacaagct gggacactat gttgttgcag agggagtgga
      661 ataccaggag cagagagatt atctggaaag gtgtggatgc gacatgcttc aaggctacct
      721 tatcagcagg ccattggatg gtgagtcggc cattaggttt attgaagagt attagtgacc
      781 aggggtcagt ccccttggaa acatattttt tatggtaact ctttaaagtg gtgggtataa
      841 tacccttaag ggtattatga ggaggttgag ccatggaatt gtcaaaggat gttttactga
      901 aggaaattca ggatctacga gatgaaaaca agaggttgag aaagcagctt gaggagatga
      961 atcccaggga taggctgacg gggttactta acagaggatc cctttatgag agacttgagt
     1021 atgaggctgt gcgtacggat agaggccagt catacctaag cctgcttcta atcagaatat
     1081 ctgatttcaa gggtcttgtc caggagcacg gcaaggatac catggatgtc cttgtaaagc
     1141 tcatgggcca ggcgatttcc gaatctgtta ggtctgttga tatagtggga agatatgagg
     1201 atggaggatt tctcttgctg ctacctgaca ttcagccaaa caagggaacg gtggtttctg
     1261 aaagactcaa aaaggcaata gacaggcaga tcagccccat gggcataaga ataaagtaca
     1321 gcataggggt caagctgtac aggaacgagc cgatcaatga gctcctgact gaaactgaaa
     1381 ggcttgcaat gagctccgag gcagaaggta ttggcagggt tcacatgtag attgaaatag
     1441 tagagaccgg gaataagctg atcaagcttc ccggtcttca atggtttttg attaatttat
     1501 aaacatctgt gtccagtaga ttgtgccgtt gtcgttgtag attccaactc ccaggtttcc
     1561 gaagttcatg ttcatgatgt ttgccctgtg tcctggtgat tccatccatg cctttacaac
     1621 tgcctcaggt gttgggtgac ccatggcgat gttctctccg gcatagttgt atttgattcc
     1681 aaactccttc atcatttgga agggtgatcc gtagtttgga gatgtatgat caaagtaatc
     1741 ataaatggcc atatcctttg atttctcata ggcaaccttt gaaagctcgg aatgcatcct
     1801 aaggggggaa agtccttcct tccctctctc cacattaacc aaatcaagaa ccctctgctc
     1861 aaagttggag tttagctcca cccttggaag ggacctgtac tgctggtatt tgatccttaa
     1921 aagaactgtc gctgcctcac ttcttgtaag tgttgcatcc cctctgaagg ctccatccac
     1981 gtagcctgtt aggatcccac ttgagtagga gttaaggacc gctgtcttta tctcggggtt
     2041 tccaagtgat gaaacctgcc tgatatcctt tataagctgc tcatattggg caaggtctgc
     2101 ctgaggtctg tagttctgat agtcaagaac attcgataca atccttgcca tatcatatct
     2161 tgttatgggc gaatcggggt ttgatgatgt atcgttgcat acaattccaa gggtctttgc
     2221 cttttctatg tagccatcag cccagtgggt tcccttggaa gggatgtcct tgtagccaag
     2281 gattgacatt gttgatttta tgaactccgc ccttgtcatt gtgttctttg gcctgaaggt
     2341 attgtcggga tatccctgga tggccccttc cctaacaagc aaattcacat tatcgtggaa
     2401 ccagtctgtg gaactcacat ccccaaatgc tgccgcctct gctgtaaatg gctgtataac
     2461 tatcattaag gccaaaataa ggccaagaat ttttctcatc atctatcaca ctcctaaaat
     2521 tattctatca ttcctaaaag gaatttacat gctaatttac acaagcaaaa acaaatacca
     2581 gggaaggctc atcattaccc acctgttttg tagactctcc tttcggtagc tggctgcctt
     2641 cttaggccct gtagctttgc gtccctgcct ctcggtaggt ttgcctttac gtttgacagt
     2701 atacaaaagg attggccttt agtatttgtc atatatgttt ttgctattca gttttcacca
     2761 tcattatatc actatggaaa ataaaatcaa ttatgtaaac taattgtaac agaaaagtaa
     2821 caaagaaaag acaaatcaca attctcaatt cataaatcac aattgattga tgatccaacc
     2881 agggtagaag actcttcgca aggctcagag tgacaaaata catgtcatcc tgagtgaaaa
     2941 cgaaggatct aggacctagt taaatctctg gcaaagtggt ttctaattgt gaaatgtgaa
     3001 ttgagaattg tgaattatta tgacatagtg aatattgctt tgcaatattc actatgtcat
     3061 aataaaagta taatacttta acggaggttg ttgccaatgt atctgaattg ctacgatgaa
     3121 gtaaagaata taaccagaga cctcgtaaag atcctaagta tcgtaaggca gggtggggag
     3181 agcgatgtag ccaaatggat ctacgattac tacatggacc tggactactt caggaataat
     3241 cccgaccagg tggtcttcca ggagaccctg aacgatgagg tcctaagaca caatgccatc
     3301 gcccttgtga agggaacaaa gggagtatca aacaggacgg ttgtgatgat gggacatatc
     3361 gacacagtag gggtggatga ttttggaacc aactggaagg ctgcctttga tcccgacagg
     3421 ctgcctgagg tccttaagga gatgggcgta tcggaggagg ttatagagga catcgaatcc
     3481 ggagagtaca tgtttgggcg gggggccctt gatatgaagg ctggcgtagc aggccacatg
     3541 tacctgataa agtactttgc tgaaaatccc catgagcttg atggaaacct tattgcaatt
     3601 gcagagtgcg atgaggagga caactcccac ggaataattt cagcccttaa ga
//
LOCUS       DASRQF010000190         2268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_891421, whole genome shotgun sequence.
ACCESSION   DASRQF010000190 DASRQF010000000
VERSION     DASRQF010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891421"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VFD79_05135"
     CDS             complement(<1..773)
                     /locus_tag="VFD79_05135"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HZH93466.1"
                     /translation="MNILVLLKQVPDTTEMKIDKEKGTLIREGVPTITNPDDLAGMEV
                     ALRLKDQYGAKVTAVTMGPPQAENMLRELYAMGADHAVLLTDRKFSGADTWATSNTLA
                     AYIKQVDFDLIIAGRQAIDGDTAQVGPQTAEKLGLPQISYVDDVLEFSKEKIVVKKAL
                     ENNYEVLECPLPCVITTLSTIAKPRYMRVMGISHAFEKDVTIKTFDDLNIDPAIIGLK
                     GSPTKVKKTFTKEVHSNSDILNLDGKSSARKVAEILMDM"
     gene            complement(786..1925)
                     /locus_tag="VFD79_05140"
     CDS             complement(786..1925)
                     /locus_tag="VFD79_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZH93467.1"
                     /translation="MNFDLSPKQAMMQKLFREFAINEVEPIAADIDEEERFPEETVRK
                     MSEIGMMGIPFESRYGGGDGDNLSYILAVEELSKVCGTTGVIVSAHTSLACNPIYTYG
                     TEEQRQKYLVPLASGEKLGAFGLTEPNAGTDAAMQQTVAVDEGDHYRINGTKIFITNG
                     GFAEIYVVFAMTDKAKGNRGISAFIMDTNMEGFSIGKTEHKLGIKGSSTTELIFKDVM
                     VPKENLLGPEGKGFTIAMSTLDGGRIGIAAQALGIAQGSLDQAVKYTKERKQFGRPIS
                     AFQNTQFTIADLQTKIDAARLLVYRAAFMKDMNRNYSSEAAMAKLFASEVAMEVTTKS
                     IQLHGGYGYTRDYPVERMFRDAKITEIYEGTSEVQKMVIASKILR"
BASE COUNT          577 a          549 c          432 g          710 t
ORIGIN      
        1 ttcatatcca taagtatctc tgcaacctta cgggccgagg acttgccatc aagattcaat
       61 atatctgaat ttgagtggac ctccttggtg aaggtcttct tgaccttggt aggtgaaccc
      121 ttgagtccaa tgattgcagg atcaatgttt agatcatcaa atgtctttat ggtgacatcc
      181 ttttcaaatg catggctgat tcccattacc ctcatatacc ttggcttggc aattgtactc
      241 aatgttgtta tgacacaggg aagagggcac tccagaacct cgtagttgtt ttctagagcc
      301 ttcttaacta caattttttc ctttgagaat tccaggacat catcaacata tgagatctgg
      361 ggtagaccta gcttttcagc agtctgtggg cctacctggg cagtatctcc atcgattgcc
      421 tgtcttccgg caataataag gtcaaaatca acctgtttga tatatgcagc caatgtattt
      481 gatgttgccc aagtgtcggc acctgaaaac ttcctgtcgg ttaacaggac agcatggtcg
      541 gcacccatgg catacagctc cctcagcatg ttctcagcct ggggtggacc cattgttaca
      601 gctgtgacct tggcaccgta ttggtcctta agcctaagtg caacctccat gccagccagg
      661 tcatctgggt tagtgattgt tggaaccccc tcccttataa gggttccttt ttccttgtct
      721 atcttcattt ccgtagtatc cggaacctgc ttcaacaata caagtatatt catgacacac
      781 ctccgttatc ttaagatttt gcttgcaatg accattttct gtacctcgga tgtaccttcg
      841 tagatttcag tgatcttggc gtccctgaac atcctttcaa ccgggtaatc ccttgtgtag
      901 ccgtatccac cgtgaagctg gattgacttt gtggtcacct ccatggcaac ctctgaagcg
      961 aacagcttgg ccattgcagc ctcggagctg tagttcctgt tcatatcctt cataaaagcc
     1021 gccctataga caagtagtct tgctgcatct atctttgtct gcagatcagc gattgtgaac
     1081 tgtgtgttct ggaatgctga tattggtctt ccaaactgct ttctttcctt tgtatacttg
     1141 acggcctggt caagagatcc ctgtgctatt ccaagggcct gggcagcgat tccgattctt
     1201 cctccatcaa gggtggacat tgctatggta aagccttttc cttccggccc aagcaggttc
     1261 tcctttggaa ccatgacatc cttaaaaatc agctcagttg ttgagctgcc cttgattcca
     1321 agcttgtgct cggtttttcc aatggagaag ccctccatat tcgtatccat tataaaggcg
     1381 gatattcccc tgttaccctt ggccttgtca gtcatggcaa ataccacgta tatctcagca
     1441 aagcctccgt ttgtgataaa tatctttgta ccgttgattc tgtaatggtc tccttcatcc
     1501 acagcaaccg tctgttgcat ggctgcatca gttccagcgt ttggttctgt tagtccaaat
     1561 gcccctagct tttcaccact cgcaagggga acaagatact tttgtctttg ttcttctgtg
     1621 ccgtatgtat atattgggtt acatgcaagt gaagtatgtg ctgagactat aacacctgtt
     1681 gttccacaaa ccttggacag ctcctctaca gctaatatgt aggacaaatt gtccccgtcg
     1741 ccaccaccat atctactctc aaaggggata cccatcatac cgatctcgct cattttcctg
     1801 actgtttcct ctggaaacct ttcctcttca tcaatatctg ccgctattgg ttcaacctca
     1861 ttgattgcaa attccctgaa aagcttctgc atcatcgcct gctttgggct aaggtcaaag
     1921 ttcattcctc tgttcaaata aatccactcc cttcctatac gttaaataat taacaaaaaa
     1981 actttaaaaa aatatcgcca ccaaatttgc tacctacaaa ttatattata gaccaacgta
     2041 aatataaatc aaggttttag ggcaaaattg ataaaaattt agcaaataat atattaaacc
     2101 aaggatatct gtaacagatg ttacggacca ttgttcaaat ccctgttaaa atatatctac
     2161 aaataacaat tttggaggga atagtatgat agaacaggta ttcaattatt caacaactga
     2221 tgatttcgca gtggagaagc tgatattcga tgacaatatc aactacat
//
LOCUS       DASRQF010000191         4350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_285205, whole genome shotgun sequence.
ACCESSION   DASRQF010000191 DASRQF010000000
VERSION     DASRQF010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285205"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VFD79_05145"
     CDS             <1..705
                     /locus_tag="VFD79_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HZH93468.1"
                     /translation="PKVAYVEMLISQGLLHDGYIYGVNSGKILPTLIHPNEELDGAVI
                     SGNCVAACDKITTYQHQNNSAILDLYNRHGKDINFVGVILTPELTTLEGKFRTCDYTA
                     KLCQTLGVDGVIVSEEGYGNPDSDLLMICKRLEDSGIKTVLITDECSGWDGMSQPLAD
                     TTPEAVAVVSTGNVSHVVELEPADRVIGSAEAIATLAGGWEGCLQEDGSMVCELNAVI
                     GATSEIGYHNLTVELY"
     gene            721..2019
                     /locus_tag="VFD79_05150"
     CDS             721..2019
                     /locus_tag="VFD79_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012799260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1762..1764,aa:Sec)
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HZH93469.1"
                     /translation="MSYKVLYYVNQFFGQVGGEDKAGMAPVMKDEKVGPAMGFDQLMG
                     EEAEVVGTIICGDNYFNENNEEAIKFIVDAVSEKKPDLVVAGPAFNAGRYGMACAGVA
                     KVVADELEIPVLTGMYKENPALDESRAKAIVVETTDSAAGMKNALATMSGLAKKILKG
                     EKLGYPEDEGYFAQGRRLTVFSDKRGSERAVEMLLARLKGEDFKSELPMPVFDRVAPA
                     AAVKDLSKAKIALVTSGGIVPNGNPDRIQSASAQKWGKYNIAGKDSLEGEFYTIHGGY
                     DPVYANELPDRVAPLDMMRKFQKEGLIGEVYEYFYTTTGTGTSVGNSIKFGVEIGKEL
                     KEAGVDAAILTSTUGTCTRCGATIVKEIERYGIPIVHMSTITTISESVGANRIVPTIA
                     IPYPVGNPNLSKVVEEDLREKLVRRAITALGTEVEETTYF"
     gene            2069..3172
                     /locus_tag="VFD79_05155"
     CDS             2069..3172
                     /locus_tag="VFD79_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93470.1"
                     /translation="MEKDIRWGKVLSYAGAFIAFLIGSGFATGQEVLQYFSSYGYGGI
                     LGVIVVFLLFLYVGADFMLVGQEKQFKKGNDIYRYYCGNVVGTFYDYFSIAFIYMSFI
                     VMIGGAGAAINQQYGLPETFGGVLIGVLAVATVVFGLGRIVDVIGKIGPAIVILTIFL
                     GISAIIKNPTGVVEAQQVIPQLTLMKASTNWFYAAGSYVGFCMLWLAAFMASMGASSI
                     SKKEAVMGSSMGALGFSVGLIIITLGIMANIEQLAGTMVPSLILAQNISGTLAIVFSF
                     IVVAGIYTTSVPLLWTVVARFAEEKTSRFRLLTVVFAAVGVFIGLRVPFDRLVNIIYV
                     INGYVGILLLAFMIVKRIRVIKERNSLSTVIEK"
     gene            3285..>4350
                     /locus_tag="VFD79_05160"
     CDS             3285..>4350
                     /locus_tag="VFD79_05160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZH93471.1"
                     /translation="MKISKKELILKGNMYKVLMTLSLPIMINNLIQTLYNLVDGIWVS
                     KISSVHFAATAFVFPVNFLFIAIGIGVSVAGTSILSQMVGADREDQAREYAAQLIGIS
                     VLLSLAFSIFGVAATPWIVRAMGGTGDLAVYSNIYLRITFLDLPFLFLFYNINSIRNA
                     QGDTVTPTVLSGISAILNMILDPIFIFTFGWGIAGAAWATLLSRALLSVYGIRLIMGD
                     ENPLRPDFKGFKFNRHILRELSRIGFPAAIGQSGAAFGFIILNGFIVSYGTATMAAFG
                     MVNRITSLIMQPAMGIGAALTAVIGQNIGARLYDRVRESFRKASLLTLVLGTVGGLLM
                     FFFRDPIVNFFIQSKDDPEVI"
BASE COUNT         1226 a          869 c         1122 g         1133 t
ORIGIN      
        1 ccaaaggttg catatgttga gatgctgata tcacagggtc ttctacacga tggatacata
       61 tatggagtca acagcgggaa gatactgcca accctgattc atccaaacga ggagcttgac
      121 ggggcagtaa tcagcggaaa ctgtgtagct gcctgtgaca agattaccac ctaccagcac
      181 caaaacaact cggcaattct tgacctttac aatagacatg gcaaggacat aaactttgtt
      241 ggggtaatcc ttacacctga gcttacaacc ctggaaggta aattcagaac ctgtgactac
      301 actgcaaaac tttgtcagac acttggagtg gacggtgtaa ttgtttcgga ggaaggctac
      361 ggaaatcctg actcagacct tcttatgatc tgcaagagac ttgaggattc aggaatcaaa
      421 acagtgctga ttaccgatga gtgctcaggc tgggacggaa tgagccaacc gctggcagat
      481 acaacacctg aggctgttgc cgttgtttcc actggcaatg taagtcatgt tgtggagctg
      541 gagcctgctg acagggttat cggtagtgca gaggcaattg caacacttgc tggaggctgg
      601 gaaggatgcc tgcaggagga cggaagcatg gtttgtgagc taaatgccgt tatcggcgct
      661 acttctgaga ttggatacca caatcttaca gttgaacttt actaaaagga ggttctggat
      721 atgtcatata aagtattata ctatgtaaat cagttttttg gacaggttgg cggagaagac
      781 aaggctggca tggcacctgt aatgaaggat gagaaggtag gtccggcaat gggctttgac
      841 cagctaatgg gagaggaagc agaggttgta ggtacaatca tctgtggtga caactatttc
      901 aacgaaaaca atgaggaagc aattaagttc attgttgatg cagttagtga aaagaaaccc
      961 gaccttgttg tggctggacc ggctttcaat gctggaaggt acggaatggc ttgtgcaggg
     1021 gttgcaaagg ttgttgcaga tgagttggag ataccggtac ttacaggaat gtataaggaa
     1081 aatccggcac tggatgaaag cagggccaag gcgatcgttg ttgagaccac tgattctgca
     1141 gcaggcatga aaaatgctct ggctacaatg tcaggcctgg caaagaaaat cctaaaggga
     1201 gagaagcttg gatatcctga agatgagggc tactttgctc aaggtaggag acttacagta
     1261 ttctccgata agagaggctc agagagagct gttgaaatgc tacttgcaag actaaaggga
     1321 gaagacttca agtcagagct tcctatgccc gtgtttgaca gggtggcacc agctgctgca
     1381 gtcaaggacc tttcaaaggc gaagatcgca cttgttacca gtggcggaat cgttccaaat
     1441 ggaaaccctg acaggataca gtcggcaagt gcacaaaagt ggggcaagta caatatcgcc
     1501 ggcaaggatt ccctggaggg agaattctat acaattcatg gaggttatga cccagtctat
     1561 gccaatgagc tgcctgacag agtcgcacct cttgacatga tgagaaagtt ccagaaggaa
     1621 ggtcttattg gggaggtata tgagtatttc tatactacaa ccggaaccgg tacatcagta
     1681 ggcaattcca tcaagttcgg agtagaaata ggcaaggagc ttaaggaagc aggagtggat
     1741 gcagcaattc taacatcaac ctgaggcacc tgtactcgtt gcggtgcaac gatagtaaaa
     1801 gaaattgaaa gatatggaat cccaatagtt cacatgtcaa caatcaccac catatccgag
     1861 tctgttggag ccaacagaat agttcctaca attgccatac cttatccggt cggtaatcca
     1921 aacctttcaa aggtggttga ggaagacctg agagagaagc ttgtcagaag agccataacc
     1981 gctcttggaa ctgaggtcga ggagactact tacttctaaa ttggaggtct tttgacctcc
     2041 aaccaatcta aaaggatggt ggagtggaat ggagaaagat attagatggg gaaaagttct
     2101 ttcctacgca ggggctttca tagccttcct gatagggtcg ggatttgcaa caggccagga
     2161 ggttctacag tacttcagct catacgggta cggaggaatt cttggtgtga ttgtggtatt
     2221 tctattgttc ctatacgtgg gagcggattt tatgctggtg gggcaggaaa agcagttcaa
     2281 gaagggcaat gacatataca ggtactactg tggaaacgtt gtgggtacct tctacgacta
     2341 cttttcaata gccttcatat atatgtcatt tatagtaatg attggtggag caggggccgc
     2401 cataaaccag cagtacggac ttccagaaac ctttggcggg gttttgatcg gagttcttgc
     2461 agtggcaaca gttgtattcg gacttggtag gattgtggat gtaataggca agataggtcc
     2521 ggcaatagta atattgacca tcttccttgg gatatcggca attataaaga atcccacagg
     2581 agtggtagaa gcccagcagg tgattccaca gcttactctt atgaaggcat caaccaactg
     2641 gttctacgct gcaggatcct atgtaggttt ctgtatgcta tggctggctg cattcatggc
     2701 ttccatgggt gccagctcca tcagcaagaa ggaggcagta atggggtcca gtatgggagc
     2761 attgggattt agcgtaggtc ttatcatcat aaccctggga ataatggcaa atattgaaca
     2821 gctggcaggt acaatggtac catcacttat actggcacag aatatcagcg gcactcttgc
     2881 catagtcttt tcctttattg ttgtggcagg aatctacact acatcagtac ccttactgtg
     2941 gacagttgtg gcaaggtttg ccgaggaaaa gacaagtaga ttcagattgc ttaccgtggt
     3001 atttgctgca gttggagtct tcataggtct aagggttcca tttgacaggc ttgtaaacat
     3061 catctatgta ataaacggct acgttggaat ccttctcctg gcatttatga tagttaagag
     3121 aatcagggtc ataaaggaga gaaactccct tagtactgta attgaaaaat agaaacacat
     3181 ttaggtatgg actggttcca tacctaattt tttttcaaca ggtacaattt tcccacaaac
     3241 actggtataa tggttatgat aattaaatat ttcggaggtc taaattgaaa atcagcaaaa
     3301 aggaattaat tctcaagggt aacatgtata aggttctgat gaccttgtca ttgccgatta
     3361 tgataaacaa cctaatacag accctataca accttgtgga tggtatatgg gtgagtaaga
     3421 tctcatcggt tcactttgca gcgacagcat ttgtgtttcc ggtgaatttt ctcttcatag
     3481 ccatcggaat tggagtttca gttgcaggga catccatact atctcagatg gttggtgccg
     3541 acagggagga ccaggcaagg gaatatgcag cccagctaat tgggatttcc gtattgctct
     3601 cactggcctt ctccatcttt ggggttgcag ctaccccatg gattgtaagg gctatggggg
     3661 ggacaggaga tctggcagtg tattccaaca tataccttag gatcaccttc ctggacctgc
     3721 cctttctctt cctcttctac aatataaact ccatcaggaa cgcccaggga gatacggtaa
     3781 cacccacagt acttagcgga atctcggcca tacttaatat gatattggat ccaatcttca
     3841 tattcacctt tggctggggg atagctggag ctgcctgggc aaccctcctt tcaagagccc
     3901 tgctatcggt ttatggaata aggctgatca tgggtgatga aaacccctta agacctgact
     3961 tcaagggctt caaattcaac agacatatcc tcagggagct ttcaagaata gggttcccag
     4021 ctgccatagg tcaatcagga gcagcatttg gctttattat cctcaatgga ttcatagtat
     4081 cctatggaac ggcgaccatg gcagcatttg gcatggtgaa caggataact tcccttatca
     4141 tgcaacctgc catgggaata ggtgctgcct tgacagctgt gattggccag aacatcgggg
     4201 caaggcttta tgatagggta agggaatcct tcaggaaggc aagtttgctg acccttgtct
     4261 tgggaactgt tggaggcctt ctgatgttct tcttcaggga ccccatagtg aacttcttta
     4321 tccagtcaaa ggatgacccg gaagtaataa
//
LOCUS       DASRQF010000192         3960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1802429, whole genome shotgun sequence.
ACCESSION   DASRQF010000192 DASRQF010000000
VERSION     DASRQF010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802429"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VFD79_05165"
     CDS             complement(<1..485)
                     /locus_tag="VFD79_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HZH93472.1"
                     /translation="MNNPYIIEERKIPLIPLRGISIFPHMVIHFDVGRDKSVQALEKS
                     MMDDSLIMLCTQINPKVEEPGFDDFYHVGTMAKVKQMLKLPGGSIRVLVEGINRGKVK
                     SLSQEEPYFEAIADIYSYESEDLEKNQDSEAAMRLVIEDLEEYIGLNPKVSSEVLYTI
                     S"
     gene            complement(532..1809)
                     /gene="clpX"
                     /locus_tag="VFD79_05170"
     CDS             complement(532..1809)
                     /gene="clpX"
                     /locus_tag="VFD79_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HZH93473.1"
                     /translation="MAKNDDKQLRCSFCGKPQDQVKRLIAGPNVYICNECIELCQEII
                     DEEFVDYNQDYELSDLPKPSGIKETLDEYVIQQERAKRNLAVAVYNHYKRINRKVSKS
                     EDVELQKSNILMIGPTGSGKTLLAQTLARILNVPFAIADATSLTEAGYVGEDVENIIL
                     KLIQAADYDIEKAEKGIVYIDEIDKVARKTENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGLDSIIQSRVGKKTMGFGAEVKSRKKN
                     IGELLKQIQPEDLIKFGLIPEFVGRLPVLATLEELDEDALVRILTEPRNALVKQYTQL
                     FSMDDIELEFEDEALTLIARKAIERKTGARGLRSIIEEKMLDIMYELPTMENVQKCIV
                     TKETIENGTEPTLVLADKKKSRTKENGSKESAS"
     gene            complement(1820..2404)
                     /gene="clpP"
                     /locus_tag="VFD79_05175"
     CDS             complement(1820..2404)
                     /gene="clpP"
                     /locus_tag="VFD79_05175"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HZH93474.1"
                     /translation="MSLVPIVVEQTNRGERSYDIYSRLLKDRIIILGSEINDVVADLV
                     VAQLLFLEADDPEKDIQIYINSPGGSVSAGFAIYDTIQYIKPDVTTICIGRAASMGAF
                     LLAAGTKGKRFALPNSDIMIHQPLGGAQGQAEDIRIQAEKIIEIRERINRILSERTGQ
                     PLDRIAKDTDRDYYMSAAEAVEYGIIDAVMERKK"
     gene            complement(2416..3711)
                     /gene="tig"
                     /locus_tag="VFD79_05180"
     CDS             complement(2416..3711)
                     /gene="tig"
                     /locus_tag="VFD79_05180"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HZH93475.1"
                     /translation="MKAVFEKKEKNTVHFNMELPAGDFEKALQQTYLKNRGRFNIPGF
                     RKGKVPRKIIEMNFGEEIFYEDAINMLLPDAYSSAIEELELEPVDSPEVDVEEIKNGE
                     PIKVNFSVEVKPEVKLGDYSEIELEKVDYSVTDEMVQAELDKARDMNSRMVDAGDREV
                     QEGDILTIDFAGFVDGDQFPGGTAEDQTLEIGAGQFIPGFEEGLVGKSKGEEVDVEVT
                     FPEEYHEESLKGKPAVFKVTIKEIKVKELPELDDEFAKDVSEFDTIEEYKNNLKEELA
                     KSLENQERIETENNLVDKVVELSEVEIPEAMIQTQVENEIKDFEYRVKNQGFELDQYL
                     ELTGTTMSDLELNFRPMAEKRVKADLVLEAIAEAEGVQVTDEDLDAELEKMADQYNQV
                     ENKDNFIEQMKKGDLGFLKAGISNTKVLELLKEKVKYIG"
BASE COUNT          961 a         1015 c          646 g         1338 t
ORIGIN      
        1 tccgaaatgg tgtagagcac ctcagatgaa accttgggat ttaaacctat atattcctcc
       61 aggtcttcaa tcaccagtct catggcagcc tctgagtcct ggttcttctc caggtcttcg
      121 ctttcatatg agtaaatgtc agctatggcc tcaaagtagg gctcttcctg acttaagctt
      181 tttacctttc ccctgtttat tccctcaaca agcaccctga tgcttccacc aggcagtttg
      241 agcatttgct tgaccttggc catggtacct acgtgataga aatcatcaaa acctggctcc
      301 tcgacctttg gatttatttg tgtacaaagc attatcagtg aatcatccat catggatttc
      361 tctagtgcct gtactgactt atccctcccc acatcaaagt gtatgaccat gtgtggaaat
      421 atagatattc ccctaagtgg tatcagagga atttttctct cttctataat ataagggtta
      481 ttcattttcg tccctcctaa atgcaaaata aaagctcaca gttgtgagcc tttatgatgc
      541 agattcctta ctgccatttt cttttgtcct acttttcttc ttgtctgcta gtacaagggt
      601 aggctctgtt ccattttcaa ttgtttcctt ggttacaatg catttttgaa cattttccat
      661 tgttggaagc tcatacatta tatcaagcat tttttcttca attatgctcc ttagacctct
      721 ggcacctgtt tttctctcta tggcctttct ggctataaga gtcagtgctt catcttcaaa
      781 ttcaagctca atgtcatcca tgctgaatag ctgggtgtat tgcttcacaa gcgcattcct
      841 gggttccgtc agaattctaa ccaaagcgtc ttcatccagt tcttccaggg ttgccaaaac
      901 cggcaatctt ccaacgaatt ctggaatcaa tccgaattta ataaggtctt caggctgaat
      961 ttgcttcaac agctcaccaa tgtttttctt ccttgactta acctctgctc caaagcccat
     1021 ggtctttttg ccaaccctgg attgtatgat actatccaag ccgtcaaatg ctccacctac
     1081 aatgaatagg atgtttgttg tgtcgatctg gatgaactcc tggtgtggat gcttcctgcc
     1141 accctgagga ggtacattgg ccacggtacc ttcaagaatc ttaagaagtg cctgttgaac
     1201 tccctctcca cttacatctc tcgtaatgga tggattctct gtttttctag ctaccttgtc
     1261 aatctcatct atatatacta tacccttttc tgccttttcg atatcgtaat cagctgcctg
     1321 aataagcttt aggatgatgt tctccacatc ctcccctaca tagcccgcct cagtaagtga
     1381 agtagcatcc gctattgcaa atggaacatt caaaattctg gccagggtct gagcaagtag
     1441 agttttacct gaacctgttg gtcctatcat aagaatatta ctcttttgaa gctcaacgtc
     1501 ctctgatttt gatacctttc tattaatcct cttgtagtgg ttgtataccg ccactgcaag
     1561 attccttttt gccctctcct gctgaattac atattcatca agtgtctcct ttatgcctga
     1621 gggcttggga aggtcactta attcgtaatc ctgattgtaa tcaacaaact cctcgtcgat
     1681 tatttcctga cacagctcga tgcactcatt gcagatatac acatttggac ctgctataag
     1741 tcttttaacc tgatcctgtg gtttcccgca gaaagagcat ctaagttgct tgtcatcatt
     1801 ttttgccatg gaatcacctc tatttctttc tctccataac agcatcgatt attccatact
     1861 ccacagcttc tgcagcactc atataataat ccctgtctgt atcctttgct attctatcca
     1921 ggggttgtcc tgttctctca gaaagtattc tgttaattct ttccctgatt tcaatgattt
     1981 tctctgcctg tattctgata tcctcagcct gtccctgagc tccacccagt ggctgatgaa
     2041 tcatgatgtc cgaattgggc agcgcaaacc tcttgccctt tgttccggct gccagcagga
     2101 atgctcccat gctagctgct ctaccaatac atatggtagt gacatccggc ttgatatatt
     2161 gaatcgtgtc atatattgca aatccagcac ttacagatcc acctgggctg ttaatataaa
     2221 tctgaatatc cttttcagga tcatcagcct caagaaacag caactgagcc actacaaggt
     2281 cagccaccac atcattgatt tctgaaccta gaattattat tctatccttt agcagtctgg
     2341 aataaatatc gtatgatctt tctcccctgt tggtctgttc tacaactatt ggaaccaatg
     2401 acatattaaa tcctcctatc caatatactt aactttttcc ttcagcaact ccaaaacctt
     2461 ggtattggat atacccgctt taaggaatcc taaatctcct tttttcattt gctctatgaa
     2521 attatccttg ttttcaactt gattgtactg gtcagccatt ttttcaagct cagcatccag
     2581 gtcctcatct gtcacctgca caccttcagc ctcagctatt gcttcaagta caaggtctgc
     2641 cttaaccctt ttttctgcca taggcctgaa gtttagctca aggtcgctca tagttgtacc
     2701 agtaagctca aggtattgat ctagctcaaa gccctgattc ttaaccctat attcaaagtc
     2761 cttgatctca ttctcaacct gagtctggat cattgcctca ggtatctcaa cctctgacaa
     2821 ctcaacaacc ttgtccacaa gattattctc agtctcaatt ctttcctgat tctcaagtga
     2881 cttggcaagc tcttccttta ggttgttctt gtattcttca atagtatcga actcacttac
     2941 atccttggca aactcatcat caagctcagg aagctcctta accttgattt cctttattgt
     3001 aaccttgaat acagcaggtt ttcccttaag tgattcctcg tggtattcct ctgggaatgt
     3061 cacttcaaca tccacttcct cacccttgga tttacctacc agaccttctt caaagcctgg
     3121 aatgaactgt cctgcaccga tttcaagagt ctggtcctct gctgttcctc ccgggaactg
     3181 gtccccatca acaaatccag caaagtctat tgtaaggatg tcgccttcct gtacttccct
     3241 atctccggca tcaaccatcc ttgagttcat gtcccttgcc ttgtcaagct ctgcctggac
     3301 catttcatcg gttactgaat agtccacctt ttcaagctca atttcgctat agtcgccaag
     3361 ttttacctca ggtttcactt caacactgaa gtttaccttt atgggttcgc cattctttat
     3421 ctcttccaca tcaacctctg ggctatccac cggctccagc tcaagctctt ctattgcact
     3481 tgagtaagcg tctggtagca acatattgat tgcatcttca tagaagattt cctctccaaa
     3541 attcatttcg atgatttttc ttggtacctt gccttttctg aagccaggga tattgaatct
     3601 tcccctgttc tttagatatg tctgttgaag agccttctca aaatcaccag ctggtaattc
     3661 catattaaag tgtaccgtat ttttttcctt cttttcaaaa actgccttca ttgatgaacc
     3721 ccctagtata tttttttatt ctgatccgaa ttttagtctc catcatagta ttataccata
     3781 tacgcaacaa ttttggtata tgtagaaaag catatcttac acattaagaa gctttcccaa
     3841 tactcatatg aaaaaaacca atggcaataa ccactggttt aatctggtgc gggaaagagg
     3901 acttgaaccc ccacgtcaat gacactagat cctaagtcta gcgcgtctgc caattccgcc
//
LOCUS       DASRQF010000193         2328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_910667, whole genome shotgun sequence.
ACCESSION   DASRQF010000193 DASRQF010000000
VERSION     DASRQF010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910667"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VFD79_05185"
     CDS             <1..764
                     /locus_tag="VFD79_05185"
                     /inference="COORDINATES: protein motif:HMM:NF042511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6544 family protein"
                     /protein_id="HZH93476.1"
                     /translation="HKRRINREVKKLKDLQDRSREGEIEKKDIAHLPEIVQKWLIKSN
                     VVGSQWKTSVHIKQKGQMRLDPDSEKWIFASATQVVGLTEPSFVWQVKAEVKPFIYVH
                     GADSYLDGVGSLQMRLLNLFKVANEKGGPKINQSSFQRYLLELPWYPQAALNRNMKWK
                     QIDKHTARVVMNHRGVPGEAYLRFDDEGDVVYTSAMRFKDTDDKAELMECIGESKEIN
                     LMDGIRMPITVHVTWRLPEKLYTWFKVQIFKLEYK"
     gene            854..1048
                     /locus_tag="VFD79_05190"
     CDS             854..1048
                     /locus_tag="VFD79_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93477.1"
                     /translation="MKLRTKLNILASIFVLVRSMRTMTKGLRRTSPVLIATSSALVTA
                     AAFIVAPKVCEEVKARKVLS"
     gene            1200..2189
                     /locus_tag="VFD79_05195"
     CDS             1200..2189
                     /locus_tag="VFD79_05195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HZH93478.1"
                     /translation="MRNSRKFILLAFVMVLTFALTACGGGEETPADGGTGSELEGAVI
                     IDGSGTVYPLMAHIAEEYMTNEESGVSVQVGRAGSSAGFKKFIPGETDFSDASRAIKD
                     EEIAQLEENGFVYGEDVMEIKLAYDGLTMVINKNNDWATEMTEQEIVDMYLAGTYNAD
                     DVVLWSDIRPEWPAEEIKFYGPNENHGTYEFFYEVILDKQDMVKTANLQQEYSTLVDL
                     VANDEYAIAFFGFGYYASNTDKLQAVKVDFGSGPVEPALETIGEDLPYAGFTRLVFTY
                     LNLEYAKEKPQVLDYAMYVVSPEGAERLAGENGFAPLPSDMYAEYYNQLEALK"
BASE COUNT          746 a          419 c          587 g          576 t
ORIGIN      
        1 tccacaagag acggatcaac agggaagtaa aaaagttaaa agacctccag gataggtcaa
       61 gggagggtga aattgaaaaa aaggatatcg ctcaccttcc tgaaatagtc cagaagtggc
      121 ttataaaatc aaacgttgtg ggaagccagt ggaagacaag tgtccatatt aagcagaagg
      181 gtcagatgag gcttgaccct gattctgaaa aatggatttt tgcttcagcc acccaggtgg
      241 taggtcttac tgagccgtca tttgtctggc aggtaaaggc tgaggtcaaa ccctttatat
      301 atgtccatgg cgcggatagc tatcttgatg gagttgggtc cttgcagatg agactcctaa
      361 acctgtttaa ggttgccaat gaaaagggtg gtcccaagat taaccaatcc tcatttcaga
      421 gatacctgct ggagctgccc tggtatccac aggcagccct taacaggaac atgaagtgga
      481 agcagataga caagcatact gcaagggttg tgatgaacca caggggtgtt cctggtgagg
      541 cctaccttcg ttttgatgac gagggtgatg ttgtctatac aagcgccatg aggttcaagg
      601 atactgatga taaggcagag cttatggaat gcataggtga atccaaggag ataaacctga
      661 tggatggaat ccggatgcca attacagtcc atgttacttg gagattgcct gaaaaactgt
      721 acacctggtt caaggttcag atattcaagc tggagtacaa ataagacttc atatgtatgg
      781 tttgacccgg taatgagggg gtattacatt ttataggaag atagttagtc tgaaaatgga
      841 gaggaaggat accatgaaac ttcgaaccaa gctgaatatc cttgcatcaa tatttgtgct
      901 tgtaaggtcg atgagaacca tgaccaaggg acttagaagg acatcaccag ttctgattgc
      961 cacaagctcg gcacttgtaa ctgcagctgc atttattgtt gccccaaagg tttgtgagga
     1021 agtcaaggca agaaaagtac ttagctagac tttagctgga tcgtcgatta cttgtaatcg
     1081 gcgatttttc ttttttgcga ttttacatac tcttaacatt ggcatcataa atccttaatg
     1141 aaggcctgtt attctatgta tgaatagaga atagaacata acctaaggag gaaattacat
     1201 tgagaaattc aaggaaattt attttattgg catttgttat ggtgctgaca tttgcactga
     1261 cagcatgtgg tggcggagaa gagacaccag cagatggtgg aactggaagt gagctagagg
     1321 gagctgtaat aatagacgga tccggaacag tatatccgct tatggctcat atcgctgaag
     1381 agtacatgac caatgaggaa tcaggagtaa gcgtgcaggt tggtagagca ggatccagtg
     1441 caggattcaa aaagttcatc ccaggagaaa cagacttctc agacgcatca agagcaataa
     1501 aggatgaaga gattgcacag cttgaggaaa acggatttgt ctatggtgag gatgtaatgg
     1561 aaattaagct tgcctatgac ggacttacaa tggtaatcaa caagaacaat gattgggcaa
     1621 cagagatgac tgagcaggaa attgtagata tgtatcttgc aggaacctat aatgctgatg
     1681 atgttgtgct ttggtcagac atcagacctg agtggcctgc agaggagata aagttctacg
     1741 gaccaaatga aaaccacgga acatatgagt tcttctatga ggttattctt gacaagcagg
     1801 acatggtaaa gacagccaac cttcagcagg aatactccac acttgttgac cttgtggcaa
     1861 atgacgagta tgcaatagcc ttctttggat tcggatacta tgcaagcaac acagacaagc
     1921 tccaggctgt gaaggttgac tttggaagcg gccctgttga gccagcactt gagacaattg
     1981 gtgaagacct tccttatgca ggattcacaa gacttgtatt cacatacctt aaccttgagt
     2041 atgcaaagga gaagccacag gtacttgact acgcgatgta tgtggtgtca cctgagggag
     2101 ctgagagact ggcaggagaa aacggatttg ctccacttcc aagtgacatg tatgccgaat
     2161 actacaatca gctagaggca ttgaaataag agaatatata aagctgtggg tggcgactta
     2221 tcatccacag ctaaacttcc gtaacaacaa aaatcagggg agataaccat ggaaaacaag
     2281 acagtaaggg aaataataca agaaaatata aagaaaaaga accatttc
//
LOCUS       DASRQF010000194         2662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1383724, whole genome shotgun sequence.
ACCESSION   DASRQF010000194 DASRQF010000000
VERSION     DASRQF010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383724"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1184)
                     /locus_tag="VFD79_05200"
     CDS             complement(72..1184)
                     /locus_tag="VFD79_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-NAD kinase family protein"
                     /protein_id="HZH93479.1"
                     /translation="MKLGVIVNPIAGMGGRVGLKGTDGPEVLERARKMGAKPESPGKA
                     EKALRTLLPLKDRLEILTFPGDMGENEMRNLGFDPLVLNHLEEETTPEDTERAARLMA
                     DQGVDLIIFAGGDGTARNVYNAIGQEVPVIGIPAGVKIHSGVFANHPGGAGEIAFNYL
                     QDLELGTKEAEVMDIDEDAFREGVVTAKLYGYMRIPHMPDLTQVQKSSGFADEKEAIG
                     GIAEKIVEEMREGIYYIMGSGTTIRPIMEDLGLPNTLLGIDIIKDKKLVASDVNEKQI
                     LDIIGNEPAKIIVTVIGGQGYIFGRGNQQISAEVIKKVGKKNIIVAAPRSKIFSLQGR
                     PLLVDTGDEEVNGMMNGYIKVVYDYYTESLQEVKGL"
     gene            complement(1197..>2662)
                     /locus_tag="VFD79_05205"
     CDS             complement(1197..>2662)
                     /locus_tag="VFD79_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HZH93480.1"
                     /translation="VLNHRILAINRGEKERVLKASLHVEGDDGVRIIRDRLLKNPPSF
                     CTGYVDSAIQDGYKRLLYPSIEREIRNDLTERAEDEAIKVFALNLEPLLMQAPIKGKT
                     VLAIDPGFRTGCKVAVVDDTGKLLDNTTVFPTEPQKKIEATKKTLGALIEKYNVELIS
                     IGNGTGSRETEAVVADLIKEIPQKVAYAIVNEAGASVYSASEVGQKDFPDHDVTVRGA
                     ASIGRRLQDPLAELVKIDPKHIGVGQYQHDLNQGKLDEALTAVVEDSVNRVGVDLNTA
                     SPSLLKYVSGVSTKVANGIVAHREENGKFNSRKQLLKVKGLGDKTFKQCAGFLRIPGG
                     KDPLDNTGVHPESYHIAKDLMVKELDSLNLKELSEKLQVGLPTLEDIVKELKKPGRDP
                     RDEMPKPLLREDILKLEDLSPDMVLNGTVRNVVDFGAFVDIGVKQDGLVHISELADKF
                     IRHPKEIVKVGDIVKVKIIQVDKDKGRISLSMKNITG"
BASE COUNT          601 a          750 c          512 g          799 t
ORIGIN      
        1 gattcctcga ctacgctcgg aatgacagct tcttttcatt gtgaatcgtg aatcgtgaat
       61 tgtgcattga tctacagtcc cttaacctcc tggaggctct cggtatagta atcatagaca
      121 acctttatat agccgttcat cataccattg acctcctcat ctccggtgtc cacaagaagt
      181 ggccttccct gaagagagaa tatcttgctt cttggagctg caacgataat gtttttcttc
      241 cccacctttt tgattacctc tgcactgatc tgttggtttc cacgaccaaa gatatatccc
      301 tggccaccta tgacagtgac gataatcttg gcaggctcat taccgattat atccaaaatc
      361 tgcttctcgt tcacatctga agcaacgagc tttttgtcct tgattatatc tattccaaga
      421 agtgtattcg gcagaccaag gtcttccata atgggtctga ttgttgtacc cgaccccatt
      481 atatagtaga ttccctctct catctcctca actatctttt cggcaattcc acctatggct
      541 tccttttcgt cagcaaatcc actggacttc tggacctggg ttaggtctgg catatgggga
      601 atcctcatgt agccatagag ctttgccgtt acaacgccct ccctaaaggc atcctcatca
      661 atgtccataa cctcggcctc cttggtgccc agctccaggt cctgtaggta attgaaggca
      721 atttctccag cccctccagg atggtttgca aatacacccg aatggatctt tacacctgct
      781 ggtattccaa tcactggaac ctcctggccg attgcattat aaacatttct ggctgttccg
      841 tctccgcctg caaatataat aaggtctacc ccctgatcag ccatcaaccg tgctgccctt
      901 tcagtgtcct caggtgtggt ctcttcctcc agatgattca aaacaagagg atcgaaacca
      961 agatttctca tttcattttc ccccatatca ccaggaaaag taagaatctc aagtctatcc
     1021 ttcaagggta aaagagtcct caaggccttt tcagcctttc caggtgattc aggcttagct
     1081 cccatctttc tggccctctc aagaacctct ggcccgtctg tcccctttaa tccaactctt
     1141 ccacccattc ctgcgatcgg gtttacaatt acgccaagct tcatcttcca gtcctcctat
     1201 ccagttatgt ttttcatgct cagggaaatc cttcccttgt ccttgtcaac ctgaattatc
     1261 ttcactttga caatatcccc aaccttaacg atttcctttg ggtgcctgat aaacttgtcc
     1321 gcaagttcag atatgtggac aagtccgtcc tgctttacgc ctatatccac aaatgcacca
     1381 aaatcaacaa cattccgcac agtcccattt aggaccatgt caggtgaaag gtcctcaagc
     1441 ttaaggatgt cctccctcag gagtggcttt ggcatttcat cccttgggtc cctgccaggc
     1501 tttttaagct cctttacaat gtcctccagg gtcggaagtc ccacctgcag tttctctgaa
     1561 agttccttga ggttcaggga atcaagttcc ttgaccataa ggtccttggc tatatggtag
     1621 gactcagggt ggactcctgt attgtccagg ggatcctttc cacctggaat ccgtagaaag
     1681 cccgcacact gtttaaaggt cttgtctccc agtcccttca ccttcaggag ctgctttctg
     1741 ctgttgaact ttccattttc ctccctatgg gcaacaattc cattggcaac ctttgtagag
     1801 actccagaga cgtatttcaa tagggatgga cttgcagtgt tcaggtctac acctaccctg
     1861 tttacagagt cctcaaccac tgctgtcaat gcttcatcca gcttgccctg gttaaggtca
     1921 tgctggtatt gaccaacgcc tatatgcttt ggatctatct taacaagttc agccagtggg
     1981 tcctggagtc ttctacctat tgaagcagct cctctgacgg ttacatcatg gtccggaaag
     2041 tccttctgtc caacctccga tgcggaatag acagatgccc ctgcctcgtt tacaatagca
     2101 taggcaacct tttgtggaat ttccttgatc aagtctgcta caaccgcctc agtctcccgt
     2161 gaccctgttc catttcctat ggaaatcagc tcaacattat atttttctat gagagccccc
     2221 aaggtcttct tggtagcttc aatcttcttc tgtggctctg taggaaacac cgtcgtatta
     2281 tccagaagct tgcctgtgtc atccacaact gcaaccttgc atcctgtcct gaagccaggg
     2341 tcgatggcaa gaaccgtctt gcccttgatt ggggcctgca tgagaagggg ctcaaggtta
     2401 agggcaaaga cctttattgc ctcatcctcc gccctttcag taaggtcatt cctgatctcc
     2461 ctttctatgg atgggtaaag aagtctcttg tagccatcct ggattgcgga atccacatag
     2521 cctgtacaaa agcttggagg gttcttcaaa agcctgtccc ttattattct taccccgtca
     2581 tctccctcaa catggagaga cgccttaagc accctctcct tctcccccct gttgattgcc
     2641 agaatcctgt ggttaagcac tt
//
LOCUS       DASRQF010000195        13211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1119013, whole genome shotgun sequence.
ACCESSION   DASRQF010000195 DASRQF010000000
VERSION     DASRQF010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 13211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13211
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119013"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1571
                     /locus_tag="VFD79_05210"
     CDS             <1..1571
                     /locus_tag="VFD79_05210"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019034648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HZH93481.1"
                     /translation="GGVVSSLGKGITAASLGQLLKSRGLKVTIQKFDPYINVDPGTLS
                     PYQHGEVFVTEDGAETDLDLGHYERFIDENLTGNNSVTTGSVYWSVISKERRGDYEGK
                     TVQVIPHITNEIKERIVRTSRYQSFDVVITEIGGTVGDIESLPFMEAIRQIKYDVGKE
                     NVMYIHVTLVPYLSKAGELKTKPTQHSVKELRSIGIHPDALICRTEKPLGRELKEKIA
                     LLCDLEFEGVIQNLDSDSIYRIPRMLEKEGLGDLVQRKLELRCEPADLGQWDSLIEGM
                     EKIQRSVKIALVGKYVELKDAYLSVCEALTHGGLANGANIDLKWINSENLNEGNVEEL
                     LGDAQGIIVPGGFGHRGIEGKISAIRFAREKGIPYLGLCLGMQLACVELARNKAGLQG
                     AHTTELDPDTPHPVIYIMPDQKNVKDLGGTLRLGSYPCDLEEGSKAREIYGSDTISER
                     HRHRYEFNMDYREILEKEGMRFSGLSPDKSLVEVVELKDHPFFVAVQYHPEFKSRPTR
                     PHPLFKAFIEASLK"
     gene            complement(1596..2918)
                     /locus_tag="VFD79_05215"
     CDS             complement(1596..2918)
                     /locus_tag="VFD79_05215"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZH93482.1"
                     /translation="MNNIDIFFMGIKNLWRRKLRTFLTVLGVIIGASSIIVMLSLGFG
                     LSQAFEDQISQWGSLTTINVQQGWSDPSMPGGAGKEDVKLNDAAVVSFKTLPHVVAVS
                     PTLETYGSIINGRYVAGVPIRGIDPDVMEEFGFEIGEGRLLAAGDELTVLFGSDMAQN
                     FYDPKARVWREPKVDLMKDRMTLTLNPNYGWEDPNIPPSEKVNYKEYKIKTAGILAGG
                     NWEHSYGVYMPITEVQKLMKEKEKAENIRPAPGQSTDSQYQRIDVKVDDMKNVKDVQD
                     TIKSMGYEAYSLNDQLEAMKETAGVIQLVLGGIGAISLLVAAIGITNTMVMSIYERTR
                     EIGIMKVIGATINDIKKLFLFESALIGLLGGIIGIGVSYLLSFGINHFGSAFGNMFGT
                     GGETKISIIPIWLVFAALLFSALIGIASGYYPARRAMNLSAIDAIRTE"
     gene            complement(2911..4863)
                     /locus_tag="VFD79_05220"
     CDS             complement(2911..4863)
                     /locus_tag="VFD79_05220"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HZH93483.1"
                     /translation="MEPKLIMVSPNTYAVEPGDNLRLDIELYNSGEYDAENIVATLSS
                     DSTGLVLVDGKSFREEEYIEPGDRETLRFDVAVNKDAPDGTYTMNLEVVYYDYYLGKE
                     LTLDKTINIRVIRDETTPDLVLSRTDVMPTKTVNPGDPVVVGFEILNKGEGPARNITV
                     SLPELSEESLLLANGLSIQRLPDIAGGRKGYVYFELKASNTIRSGNHQMMVQISYRDG
                     DNKTVESQEYFSFQATKKDERASKLVIENLSYPTGALGQNREVKVTFDVRNQGQSPAR
                     DIEIRATSQDESGLVPKSLGITKTEEIQPGELKSYQFLFVTTRTGTTRNYPIEITVDY
                     TDELVEPASRETLTHLIGVFLNAPDEDANQSTPKLIIDKYSFSPLLISAGEQFQMNLS
                     FFNTNSSKTVRNIKIYLTADEETESGSVFTPVNSSNTFYIDSIPPKGKVEKTITMYTV
                     PDAKAKTYTLTANFEYEDSLANPYTAVELIGVPVVQQSKLDTGEVGFFPESYVGQSTP
                     ISVEFYNTGKVPLYNMMVKLEGDFQTENGQYYVGNFNSGSSDYFEGYVIPSEPGQVQG
                     EVVFTFEDSTGQMIEERKPFSLNVMDMAPMPDFPMEPPMEEPGVGGSLSRFLIPGLVV
                     LAAIGGIVFYRRRKAKKFDEDLEIDE"
     gene            complement(4954..5619)
                     /locus_tag="VFD79_05225"
     CDS             complement(4954..5619)
                     /locus_tag="VFD79_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZH93484.1"
                     /translation="MGDEKIRALDDVSLSIAKREFICLIGTSGSGKSTLLNMMAGLEK
                     PTKGEIIVDGHHLEKMNERQLTKFRQLNVGFVFQSYNLIPTLTALENVSLVLTFKGVP
                     RASREKAAAKMLRNVGLGDRLHHRPSQMSGGQQQRVSIARAFVSNPRIVFADEPTGNL
                     DTRTTFEVMDLITGMAKEFNQTLVIVTHDVEISDYAHKIVHLRDGNIEKIVVQENHKE
                     ATE"
     gene            complement(5786..7003)
                     /locus_tag="VFD79_05230"
     CDS             complement(5786..7003)
                     /locus_tag="VFD79_05230"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HZH93485.1"
                     /translation="MKLKGNALVAQGGGPTAVINQSLVGVVLEARKFSQVEKIYGALH
                     GVSGIINEEFLDLTQETTNNFERIARTPGSALLSTRDKPDDDYCDKIFKVLEAHNIRF
                     FFYIGGNDSADTVRIVNQKAMESGYELRAIHIPKTIDNDLLVNDHCPGYGSAARFVAQ
                     AFIGVNLDVNSFGGVYVGIVMGRNAGFLTAAASMAQKYPDDGPHLIYLPERPFIVDNF
                     LADVKEVYEKQGRCVIAVSEGIVDEEGTPIIASLQEEVERDSHGNIQLSGTGALGDLL
                     ADTIKKRLGIKRVRSDTFGYLQRSFIGCVSDVDQQEAREVGEKAAQYALWNNVDGSVT
                     IHRTGIYSVDYQLSPLSDVAKYTKSMPDEFINEAGNHVTEAFKYYLLPLLGSDMPQAH
                     RIRAPRVDKKLNR"
     gene            7202..7585
                     /locus_tag="VFD79_05235"
     CDS             7202..7585
                     /locus_tag="VFD79_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HZH93486.1"
                     /translation="MDKVMIRMRMSAHDAHYGGNLVDGARMLGLFGDVATELLIRNDG
                     DEGLFVAYDMVEFTAPVYAGDYIEAVGEITKLGNTSRKMKFEARKVIVPRPDINDSAC
                     DFLEEPIVVARAEGTCVVPKDKKRK"
     gene            7865..8686
                     /locus_tag="VFD79_05240"
     CDS             7865..8686
                     /locus_tag="VFD79_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HZH93487.1"
                     /translation="MEKLIITAAISGAEVTKEHNPNIPYTVEEIGREAESAYKAGASI
                     IHLHVREDDGTPTQNKERFKACMDEIRRRCPDVIIQPSTGGAVGMTDEERLQPTELYP
                     EMATLDCGTLNFGGDEIFVNTENTIKNFGRIMIERDIKPEVEVFDKGMIDYAIKFQKQ
                     GFIKAPMHFDFVLGVQMTATARDLAFMVDSIPQGSTWTAAGIGRNQFPIAAMAIAMGG
                     NVRVGFEDNTYISKGVLAKSNGEMVEKVVRIANELGREIATPAEAREILGLKPLK"
     gene            8762..10366
                     /locus_tag="VFD79_05245"
     CDS             8762..10366
                     /locus_tag="VFD79_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HZH93488.1"
                     /translation="MRFDPYKYQYPSRRNLVYGGRGMVATTNPYASQAGLDMIKAGGN
                     AFDGAVAAAAVLTVVEPTSNGLGSDAFAILYSGGRLHGINGSGHSPEALTMEYIRDKG
                     LDRVPMHGSLPVMVPGAVGAWAEIIDKCGNLTLEEVLQPAIRLAEEGYCVQATIAENW
                     ARGLTIQETLKEDHFRHWYDSFTKGGRTPYPGELWKSPDMGKTLRSIARTGGEDFYRG
                     ELAQRISEFLQESGGVMTVEDLASYRPEWVEPLSVNFRGYDIWELPPNGHGINVLLAL
                     DIMKNLPQGGFDDPESVHRAIEAMKLGFEDGMPLVGDPRFCQDRWVDLLKGENGVKRA
                     GEITGFARTPLCGDPGKGGTVYLAAADGEGNMISFIQSNFRGFGSGLVVPGTGISLNN
                     RGIDFSMDNHSPNALMGRKRPYHTIIPGFITKNGKAIGPFGVMGAYMQPQGHLQVLLN
                     TIDYGMNPQSALDAPRWQWTGGMEVHIEEDFPSEIAQVLEGKGHRIAINRDTSFFGRG
                     QMIIRTGEGQLCGATEKRADSSIAVW"
     gene            10578..11921
                     /gene="rho"
                     /locus_tag="VFD79_05250"
     CDS             10578..11921
                     /gene="rho"
                     /locus_tag="VFD79_05250"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HZH93489.1"
                     /translation="MDKEILEVKKLSELKEIAKAYDIPGYYRMKKDELIQTLTNHGSE
                     KNEDLVKEIGGDDIEITDDLSDNASEEMDSLDEKDIAEGILEMHQDGYGFLRRDNYLS
                     GDDDIYISPSQIRRFRLQTGDKITGITRPPKSNEKFNALLYIKAVNDMPPATAINRPD
                     FDSLTPIYPIERLQLETDGREYATRMIDLVAPIGKGQRGMIVAPPKAGKTTLLKMIAN
                     GIAKNHPEVEIIILLIDERPEEVTDMKRSVKGDVVYSTFDELPKNHTKVAEIVLERAK
                     RLVEHGKDVVILLDSITRLARAYNLSIPATGRTLSGGLDPGALHKPKRFFGAARKMEE
                     GGSLTILATALVETGSRMDDVIFEEFKGTGNMEVHLDRKLSEKRIFPAIDIYKSGTRR
                     EDLIMSQKELETVWALRKALGNSNTQDVTEAFIGNLVQTKNNKDFIDQMRNKIWV"
     gene            12048..12251
                     /gene="rpmE"
                     /locus_tag="VFD79_05255"
     CDS             12048..12251
                     /gene="rpmE"
                     /locus_tag="VFD79_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HZH93490.1"
                     /translation="MKKDIHPEYKEVTVTCACGNTFKTGSTKDELRVEICSECHPFFT
                     GKQKFMERGGRVEKFKKKYQIED"
     gene            12316..>13211
                     /locus_tag="VFD79_05260"
     CDS             12316..>13211
                     /locus_tag="VFD79_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HZH93491.1"
                     /translation="MNKLKNAPIEAKHKTSIGGQAIIEGVMMRGPEKIAIAVRKPDKE
                     IEIKVNPVESVARKYKVFGLPFVRGMVGLIEALKIGTGALLYSAEFFEDEQTLDKDGN
                     PKKTITQKLFGEKAQDVEMALAVVLSVAMTVGIFMVLPNIITNFLKGNIQNTVTLNLI
                     EGIIRISIFLLYVVWVSKLEDIKRVFQYHGAEHKSIHCYENEMELTVENVRKFPILHA
                     RCGTSFLFMVMAVSIVVLSFFGWPNPVLRILTRILMFPVIAGISYEINRFIGRSDSRL
                     CYALSYPGLMIQKYATVKEPDDS"
BASE COUNT         3667 a         2839 c         3238 g         3467 t
ORIGIN      
        1 caggaggagt cgtatcatct ctaggtaagg gaataaccgc agcaagcctt ggacagctat
       61 tgaaaagccg tggattgaag gttacaatcc agaaatttga tccatatata aatgtcgatc
      121 caggcaccct tagcccatac cagcatggag aggttttcgt aacggaggat ggagctgaga
      181 ccgaccttga cctgggtcat tatgagaggt ttatcgacga gaatctaacc gggaacaaca
      241 gtgttaccac aggttcagtc tactggtctg taatatcaaa ggaaagaaga ggggactatg
      301 agggaaagac cgtccaggtt atcccccaca taacaaacga gataaaggag agaatcgtaa
      361 ggacatccag atatcagtcc tttgatgttg taataactga gattggtggt accgtaggag
      421 acatagagtc gctacctttc atggaagcaa tcagacagat aaagtacgat gtaggcaagg
      481 aaaatgtaat gtatatacac gttacccttg ttccctacct ttccaaggct ggtgaactaa
      541 agaccaagcc aacccagcat agtgtaaagg agctaaggag cattggcatc catccggatg
      601 cactgatctg caggacggaa aaaccactgg ggagggagct taaggaaaag attgcactgc
      661 tttgcgacct tgaatttgag ggggtaatcc agaacctgga ttctgacagt atatacagga
      721 ttcccagaat gcttgagaag gagggattgg gagaccttgt ccagagaaag cttgagcttc
      781 ggtgtgaacc ggctgaccta ggccagtggg acagtcttat tgaagggatg gaaaaaattc
      841 aaaggtcggt taaaatagcc ctggttggaa aatatgtgga gcttaaggat gcctaccttt
      901 ccgtatgtga agctttgacc catggtggcc tggcaaatgg tgccaacata gatcttaaat
      961 ggattaattc agagaatctc aatgagggga atgtagaaga gctccttggg gatgctcagg
     1021 gaatcattgt acctggaggc tttggtcaca gggggataga ggggaagatt tcagccataa
     1081 gatttgcaag ggagaagggg attccatacc tcggcctttg cttgggaatg cagcttgcat
     1141 gtgttgagct tgcaagaaac aaggcgggtc ttcaaggagc ccacacaact gaactggacc
     1201 ctgacacacc ccacccagtc atatatatca tgccagacca gaagaatgta aaggatttgg
     1261 gtgggaccct aagacttggc tcatacccct gtgatttgga ggagggatcc aaggccaggg
     1321 aaatctatgg cagtgacacc ataagtgaga gacacaggca tagatatgag ttcaatatgg
     1381 attatagaga gattctcgaa aaggaaggga tgaggttctc gggactttct cccgataaga
     1441 gccttgtgga ggtggttgag cttaaggatc atccattctt tgtggcggtc cagtatcatc
     1501 cggagttcaa gtcaagacca acaagaccgc atcccctgtt caaggcattt atagaggcat
     1561 cattgaaata gaaaagctcc caatgggagc ttttctcatt ccgtccttat tgcatctatt
     1621 gccgataggt tcattgccct tcttgcaggg tagtagcctg aggctattcc aatcagggct
     1681 gaaaacagaa gtgctgcaaa gaccagccat attgggataa ttgaaatctt tgtttcccct
     1741 cctgtgccga acatgtttcc aaaggcactt ccaaagtggt ttatgccaaa ggataggaga
     1801 tagcttacac ctatgccgat gattccacca agcagtccta ttagggctga ttcaaagagg
     1861 aagagcttct ttatatcgtt gattgttgca ccgatcacct tcattatgcc gatttccctg
     1921 gttctttcat aaatactcat gaccattgta tttgtaatcc cgatggctgc aacaaggagt
     1981 gaaatggcac cgattcctcc aaggacaagc tggatcactc cagcggtttc cttcatggct
     2041 tccagctggt cgttcagact gtaggcctcg tagcccatgg actttatggt atcctggaca
     2101 tccttcacat tcttcatgtc atccaccttg acatcaatcc tttggtattg agagtcggtg
     2161 gattggcctg gagccggtct gatgttctca gccttctcct tctccttcat aagcttctga
     2221 acctcagtaa tgggcatata gactccatag ctgtgttccc agttgcctcc tgccaggatt
     2281 cctgcagtct tgatcttgta ctccttgtag ttgacctttt cagatggggg aatgtttgga
     2341 tcttcccagc catagttggg gttaagggtc aaggtcatcc tgtctttcat aaggtctacc
     2401 ttgggctctc tccatacccg ggccttgggg tcgtagaagt tttgcgccat gtcgctgccg
     2461 aaaaggacag ttagctcatc gccagctgca agaagtctac cttcacctat ctcaaatcca
     2521 aactcctcca ttacatccgg atcgattccc ctgatgggta ctccggccac ataccttcca
     2581 ttaataatgg acccgtaggt ctcaagggta gggcttacgg ccaccacatg gggaagggtc
     2641 ttgaagctca caacagctgc gtcgttaagc ttgacatcct ccttgccagc acctccgggc
     2701 atggaggggt cggaccatcc ctgctggaca ttgattgttg taaggcttcc ccactgggaa
     2761 atctggtcct cgaatgcctg ggaaagtcca aaaccaagac ttagcatgac tataatggag
     2821 cttgccccta tgattacccc caggactgta agaaaggttc ttagctttct tctccacaga
     2881 tttttaattc ccataaaaaa gatatcaatg ttattcatct atttccagat cctcatcaaa
     2941 tttttttgcc ttcctcctgc gatagaatac aatgccgcct atggccgcaa ggaccaccaa
     3001 gccagggatc aggaatctgg aaagagaccc tccaactcca ggctcctcca tggggggctc
     3061 cattggaaaa tcaggcatgg gcgccatatc catgacattt aggctaaagg gcttcctttc
     3121 ctctatcatt tgacctgtgg agtcttcaaa tgtgaagacc acctcgccct ggacttgtcc
     3181 aggctcgcta ggtattacat agccctcaaa gtagtcactg ctgccgctgt tgaaattacc
     3241 aacgtagtac tgaccattct cagtctgaaa gtctccctcc agcttgacca tcatattata
     3301 caaggggacc ttaccggtat tgtagaactc aactgaaatg ggagttgatt gccccacata
     3361 ggattcaggg aagaagccca cttctccagt gtcaagcttt gattgctgaa caactggaac
     3421 tcctataagc tcaactgctg tgtagggatt ggccaggctg tcttcgtact cgaagtttgc
     3481 agtgagggta taggtcttgg ccttggcatc cggtacggtg tacatggtta tggtcttctc
     3541 gactttcccc ttgggtggga tgctgtcaat ataaaaggtg tttgagctgt tgaccggtgt
     3601 aaatactgat ccagattcgg tttcctcatc agctgtcagg tatatcttta tgtttctgac
     3661 ggtttttgag ctgtttgtat taaagaagga caagttcatc tggaactgtt cccccgcact
     3721 tatgaggagg ggggagaagc tgtacttgtc gataatcagc ttaggtgtgg actggttggc
     3781 atcctcatct ggagcgttga gaaaaacgcc gatcagatgg gtcagggtct ctcttgatgc
     3841 aggctccacc aactcatcgg tataatccac tgttatttca attgggtagt tcctggtggt
     3901 tccagtcctt gttgttacaa agaggaactg gtatgactta agttctccag gttggatctc
     3961 ttcagttttt gttattccca gcgactttgg aacaagtccg ctttcatcct ggctggtggc
     4021 cctgatttca atatcccttg caggggactg gccctggttt ctaacatcaa aggtaacctt
     4081 gacctccctg ttctgtccaa gggctccagt tgggtaggag aggttctcta ttaccagctt
     4141 ggatgccctt tcatccttct ttgtggcctg gaaggaaaag tattcctggg attccacagt
     4201 tttgttgtcc ccatcacggt agcttatctg aaccatcatt tggtgatttc cagacctgat
     4261 tgtgtttgat gccttaagct caaagtatac atatcccttt cggccacctg ctatgtcagg
     4321 aagcctctgg atgctaagac cattggcaag aaggagtgac tcctccgata gctcggggag
     4381 acttactgta atatttcttg caggcccttc tcccttgttg agaatctcaa agcccacaac
     4441 aacaggatca ccagggttca cagtctttgt aggcataaca tcggtccttg aaaggacaag
     4501 gtccggagtg gtttcatccc ttataaccct tatgtttatt gtcttatcca gagtcagttc
     4561 cttgcctagg tagtagtcat agtatacaac ttccaggttc atggtgtagg ttccatcagg
     4621 agcatccttg tttactgcta catcaaacct taaggtttcc ctatcacccg gttctatata
     4681 ttcttcctcc ctaaaactct tgccatccac caatacaagt ccagtgctgt cggaggacaa
     4741 ggtggcaact atgttttcag catcatactc accgctgttg taaagctcaa tatccaaacg
     4801 caggttatca cctggttcca ctgcataggt gtttggactt accataatta gcttgggttc
     4861 catatacagg ggcccctcac tagccagaga ggtaagtggt aaaagggtaa aaatcataat
     4921 aactgcaatt atggtgctca atgaccgttt catctattct gttgcctcct tgtggttttc
     4981 ttgtactact attttttcta tgtttccatc ccgaagatgg acaatcttgt gggcatagtc
     5041 cgatatttca acatcgtggg taacgataac aagggtttgg ttgaattcct ttgccatccc
     5101 tgttatgaga tccattacct caaaggtggt ccttgtatcc aggtttcccg ttggctcgtc
     5161 agcaaataca atccgcgggt tgcttacaaa tgccctggct atactgaccc tttgctgctg
     5221 acctccactc atctgggagg gcctgtggtg gagcctatcg ccaagcccga cgtttctgag
     5281 cattttggct gccgccttct cacgggaagc ccttggaacg cccttgaagg tcagtacaag
     5341 gcttacattt tcaagtgctg tgagggttgg tatcaggttg taggactgga atacgaagcc
     5401 cacattcagc tgcctgaact ttgtaagctg cctttcattc attttctcca gatggtggcc
     5461 atccacaatg atctcaccct ttgtgggctt ctcaaggccc gccatcatgt taaggagggt
     5521 actcttgcca gaacccgagg ttccgatcag gcagataaac tctctctttg ctatggacag
     5581 gctgacatcg tcaagggccc ttatcttttc gtcccccatc cgatatatct ttcttacatt
     5641 gttaagtctg ataaattcat ccagaatgac gcacctcctc ttttgacaat ttaagtctac
     5701 cacaataaca aggaatatta gttacaaaat gtttaaaatg aaacaaaaaa agattgcagg
     5761 gcctccctgc aatccgatcc gtcatttatc tgtttagttt tttatccacc cttggtgcac
     5821 gaattctgtg ggcctgtggc atgtctgacc caagaagtgg aagaagataa tatttgaagg
     5881 cttcagtaac atggttgccc gcttcattta tgaactcatc aggcattgac ttggtgtatt
     5941 tggcaacatc tgacagtggt gaaagttggt aatcaactga atagattcca gtcctgtgga
     6001 ttgtaaccga tccgtcaaca ttgttccaaa gggcatactg tgctgccttc tccccgactt
     6061 cccttgcctc ctgctggtca acatccgaga cacatccaat aaaggacctt tggagatatc
     6121 cgaaggtatc gcttcttacc ctctttatgc ccagtctctt cttgatggta tcagccagaa
     6181 ggtcacccag ggctcctgtt cctgacagtt ggatgtttcc atgactatcc ctttcaacct
     6241 cttcctggag gcttgcgatt atgggagttc cctcttcatc cacaataccc tctgacactg
     6301 cgatgacaca ccttccttgc ttttcgtaga cctctttgac atcggcaagg aaattatcaa
     6361 cgataaatgg tctttcaggt aggtagatca ggtgtggtcc gtcatctgga tacttctgtg
     6421 ccatggaggc tgcagccgtt aggaatcccg catttcttcc cattacgatt cccacataga
     6481 caccaccaaa tgaattcaca tcaagattca cacctataaa ggcctgtgcc acaaatctgg
     6541 ctgcagaacc atagccggga cagtggtcat tgaccagaag gtcattgtct atagtctttg
     6601 gtatatgtat ggcccttaac tcataccctg actccatggc tttctggttt actatcctaa
     6661 ctgtgtctgc ggagtcattt cctcctatat aaaagaagaa tcttatattg tgtgcctcca
     6721 aaaccttgaa tatcttgtca caataatcat catccggttt gtccctggtg gacaatagtg
     6781 ctgatccagg tgttctggca atcctttcaa aattattcgt tgtctcctgg gtcaaatcaa
     6841 ggaattcctc attgattatt cctgaaaccc catgaagtgc gccgtagatt ttctccacct
     6901 gtgaaaactt cctggcctca agtacaactc caactagcga ttggtttatt acagcggttg
     6961 gtcccccgcc ttgtgctacc agtgcattac cctttagttt catagtcagc cctccttatt
     7021 ttcaagcaaa aaaacaattg tgatcccaat tgaagcctta acatatttat acctccataa
     7081 taccagattc aacccttttc ttcaatcagg acgccacttt gggtgaaaac gtttttccgg
     7141 tagtttactt atgaggattg tgttataatt gagacaaaca atgattaagg gggatttttc
     7201 aatggataag gtaatgatta ggatgagaat gagtgcccac gatgcccact acggtggaaa
     7261 tttggttgac ggggcaagga tgcttggtct gtttggagat gttgccactg aactgcttat
     7321 cagaaatgat ggagacgagg gtcttttcgt tgcatatgac atggttgagt ttactgcacc
     7381 tgtctatgca ggggattata tagaggcagt tggtgaaata accaagcttg ggaacacatc
     7441 cagaaaaatg aagtttgagg ccagaaaggt tatagttcca agacctgaca tcaacgactc
     7501 ggcctgcgat ttcctggagg agccaatagt cgttgcaagg gctgaaggaa cctgtgtggt
     7561 gccaaaggat aagaagagga agtagtgcta attcacaatt caaaatgcac aatgcacaat
     7621 tggaaaagat agttgacagg aattaagatt taagaagtaa tatatgcaaa attgattgtt
     7681 gttgacagag ggtaaagatt cttcactggg ttcagaatga caaaatatag tcatctcgag
     7741 cttgtcgaga gatctcagac ctcaaagctt gaccagaaca attgtgaatt gtaactagga
     7801 ttttatctat taattgtgaa ttgagaactg tgaactgaga attggaatta tgggaggtag
     7861 gataatggaa aaacttatta taaccgctgc aatatctgga gctgaggtta caaaggaaca
     7921 caatccaaac ataccgtata cggttgagga aataggtagg gaggcggaat cagcatacaa
     7981 ggctggtgcc agcatcatcc acctgcatgt gagggaggac gacgggactc cgacacaaaa
     8041 caaggagcgg ttcaaggcct gcatggatga gataagaaga agatgtccgg atgtaatcat
     8101 ccagccttca acaggtggag ccgttggaat gactgatgag gaaagacttc aaccaactga
     8161 gctttaccct gaaatggcca ctcttgactg tggaaccctt aacttcggtg gggacgagat
     8221 attcgtaaac actgaaaata ccatcaagaa ctttggtagg ataatgattg aaagggatat
     8281 caagcctgag gtggaggtgt ttgacaaggg gatgatcgat tatgcgatca agttccagaa
     8341 gcagggattt ataaaggctc caatgcactt tgattttgtt cttggggtcc agatgacagc
     8401 aactgccagg gaccttgcct ttatggttga cagtattccc cagggatcca cctggactgc
     8461 tgctggaata ggaagaaacc agttcccaat agctgcaatg gcaattgcca tgggtggcaa
     8521 tgtaagggtt ggttttgagg acaacaccta tatttcaaag ggagtccttg ccaagtcaaa
     8581 cggggaaatg gtggaaaagg tggtcaggat tgcaaatgag cttggcaggg agattgcaac
     8641 tcctgcagag gcaagggaga ttcttggact gaagcccttg aagtaaaatg catataatgg
     8701 aaagatagat atccctaggg gtatctattt ttctaaccaa tatggaaagg aagtgtgaac
     8761 ggtgagattt gacccataca agtaccagta cccatccagg agaaacctgg tctatggtgg
     8821 cagaggaatg gtggcaacca caaatcctta cgcatcacag gcaggacttg atatgatcaa
     8881 ggcaggagga aatgcctttg acggtgcagt tgcagcagca gctgtcctga cggtggttga
     8941 gccaacaagt aatgggcttg gctcagatgc ctttgcaatc ctctattctg gaggcaggct
     9001 ccatggtata aatggaagcg gccattcccc agaagccctt actatggaat acataaggga
     9061 caaggggctt gacagggttc caatgcacgg ttctttgccg gtgatggtcc cgggagctgt
     9121 aggagcatgg gctgagataa ttgataaatg cgggaacctg acactggagg aggtcctcca
     9181 gcctgccata aggcttgcag aagaggggta ttgcgtccag gctacaattg cagagaactg
     9241 ggcaaggggt ctgaccatcc aggagaccct taaggaggac catttcagac attggtatga
     9301 ttccttcaca aagggtggaa ggacgccata tccaggtgag ctatggaagt ctcccgacat
     9361 ggggaaaacc ctcaggtcaa ttgcaagaac agggggagag gacttttaca gaggggaact
     9421 tgcacaaagg atctcagaat ttttacagga gtcaggtgga gtaatgaccg tggaggacct
     9481 tgcaagctac aggccggagt gggttgagcc cttgagtgtg aatttcagag gctatgacat
     9541 atgggagctt cctccaaacg gtcatgggat aaatgttctt cttgcccttg atataatgaa
     9601 aaaccttccc cagggagggt ttgacgaccc ggaatctgtc cacagggcaa ttgaagccat
     9661 gaagctgggc tttgaggatg ggatgccact tgttggggac ccaagatttt gccaggatag
     9721 gtgggtggac cttctgaagg gagaaaacgg cgtaaaaaga gccggagaaa taacaggctt
     9781 tgccagaacc ccactttgtg gtgatcccgg caagggaggg actgtgtact tggcggctgc
     9841 agatggtgag ggtaacatga tatcatttat ccagagcaat ttcagggggt ttggatccgg
     9901 acttgtcgta cctggaactg gtatatccct aaacaacagg gggatcgact tttccatgga
     9961 taatcatagt ccaaatgcac ttatggggag gaaaaggccc taccacacca ttatccctgg
    10021 attcataaca aaaaatggca aggcaattgg tccctttgga gtcatgggtg cctatatgca
    10081 gccacaggga catctccagg tcctgcttaa taccatagac tacggaatga atcctcaaag
    10141 cgccctggat gcgcccaggt ggcagtggac agggggaatg gaagtccata ttgaggagga
    10201 tttcccaagt gaaattgcac aagtgcttga agggaagggc cataggattg caataaaccg
    10261 ggacacctca ttttttggaa ggggtcagat gataatcagg accggggagg ggcagctttg
    10321 cggggctaca gaaaaaaggg cagacagctc aattgcagtc tggtaaccat attgtaactt
    10381 caaattgtcc atattgtgtg atataatgta gattgaacaa cttcgaataa tcatttctcg
    10441 aattacttgc acaataatat tcccgaaata attacaagaa cataatgatt tacagaacct
    10501 tgtccagcaa ggttctgtat ggagtttatt tataggatac aggaaaggga tcaatattta
    10561 ggaagcgggg tgtagctgtg gacaaggaga tactagaggt caagaagcta tcggaactta
    10621 aggaaattgc aaaggcttat gacataccag gatactatag gatgaaaaag gacgaactga
    10681 tccagacatt gacaaaccat ggttctgaaa agaatgagga ccttgtgaag gaaattggtg
    10741 gagatgacat tgaaatcaca gatgaccttt ctgacaatgc ttcagaggaa atggacagcc
    10801 tggatgaaaa ggacattgca gagggtattc ttgaaatgca ccaggacggg tacggcttcc
    10861 tgagaaggga caactacctg tcgggagatg atgatatcta catatcccca tcccagatca
    10921 gaaggttcag acttcagact ggtgacaaga taacaggaat aacaaggcca ccaaaatcaa
    10981 acgagaagtt caatgcactt ctatatatca aggcagtcaa tgatatgccg ccggccacag
    11041 ccataaacag acctgacttt gacagtctta cccccatcta tcccatagag agacttcagc
    11101 ttgaaacaga tggaagggaa tacgcgacca ggatgataga ccttgttgcc ccaataggga
    11161 agggccagag gggtatgata gtggcacctc ccaaggcagg caagaccacc ctgttgaaga
    11221 tgattgcaaa tggaattgca aaaaaccacc ccgaggttga aataataata cttcttatag
    11281 atgagcgacc tgaagaggtc actgacatga agaggtcggt caagggagat gttgtctatt
    11341 caacctttga cgagctgcct aaaaaccata ccaaggttgc tgaaatagtc cttgaaaggg
    11401 caaagagact tgttgaacac ggcaaggatg tggtgattct actggacagc atcacaagac
    11461 ttgcaagggc atacaacctg tcaatccctg ccactggaag gaccctctca ggaggacttg
    11521 atccaggagc cctccacaag ccaaagaggt tttttggagc tgccaggaag atggaggagg
    11581 ggggcagcct gacaattctt gcaacagccc ttgtggaaac aggaagcagg atggatgatg
    11641 ttatattcga ggagttcaag ggtacaggta acatggaggt acatcttgac aggaagcttt
    11701 cagaaaagag gattttccct gcaattgaca tctacaagtc gggaaccaga agagaagacc
    11761 tgatcatgag ccagaaggaa cttgagactg tatgggcttt gagaaaggct cttggtaaca
    11821 gcaataccca ggatgttaca gaggccttta tcggaaatct tgtacaaacc aagaacaaca
    11881 aggacttcat tgaccagatg agaaacaaga tttgggtcta gtcaaaaaag tttaattgaa
    11941 ataatctttg aagttgaaat ttatataatc atatgctata atttagtggt tatatcgtag
    12001 agtggctaaa attatagata ctcgaaatcg aagtgaggtg aaatgagatg aaaaaagata
    12061 tccatccaga atataaagag gtgacagtta cctgtgcatg tggaaacaca ttcaagactg
    12121 gctcaacaaa ggacgagctt agggtagaaa tctgttcaga gtgccatcca ttctttactg
    12181 gtaagcaaaa gtttatggaa cgtggtggac gtgtagagaa attcaagaag aaatatcaaa
    12241 tcgaggatta atttctttac aactgggtta gttgcatgtg cacctaacct ttattttttt
    12301 tgaggaagtg aacctatgaa taaattgaaa aatgccccaa tagaagccaa gcacaagacc
    12361 tcaataggag gccaggcaat aattgaaggg gtaatgatga gagggcctga aaagattgca
    12421 attgcagtta gaaagcctga caaggaaatt gagataaagg ttaatccggt agaatccgtg
    12481 gccaggaagt acaaggtgtt cggacttccc tttgtaaggg ggatggtggg tttgattgaa
    12541 gccttgaaga tcggtacagg agccctgctc tactctgccg agttctttga ggatgagcag
    12601 acccttgaca aggatgggaa tcccaagaag accatcaccc agaagctctt tggagaaaag
    12661 gcacaggatg ttgagatggc cctggcagtg gtactttcag tggcaatgac cgttggtatc
    12721 tttatggttc ttccaaacat cataaccaac ttcctgaagg gaaacatcca gaacacagtg
    12781 accctgaacc ttattgaagg aatcatcaga atttccattt tcctattgta tgtagtttgg
    12841 gtgtccaagc ttgaggacat taaaagggtt ttccaatatc acggagcaga gcacaagagc
    12901 atacactgct atgagaacga gatggaactc accgttgaga atgtaaggaa gtttccgatc
    12961 ctccacgcaa gatgtggaac aagctttctc tttatggtaa tggcagtgag tattgtggtc
    13021 ctatccttct ttggatggcc caatccggtc ttgaggattc tcacaaggat ccttatgttt
    13081 cctgttattg ccggaatatc ctatgagatc aacagattta tcggaagaag cgattcaaga
    13141 ctttgctatg ccctaagcta tccaggtctt atgattcaga agtatgcaac agtcaaggag
    13201 ccggatgact c
//
LOCUS       DASRQF010000196         4492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_502897, whole genome shotgun sequence.
ACCESSION   DASRQF010000196 DASRQF010000000
VERSION     DASRQF010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502897"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /gene="obgE"
                     /locus_tag="VFD79_05265"
     CDS             <1..1093
                     /gene="obgE"
                     /locus_tag="VFD79_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004825595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HZH93492.1"
                     /translation="KRSYAGENGEDGRAKKQFGKDGSDTYLKVPVGTLIKDEETGKVI
                     VDLKEPGQEFVIARGGKGGRGNAKFATSTRQAPGFAEAGTKGQERKIVLELKLLADVG
                     LIGFPNVGKSTILSTISAAKPKIANYHFTTLKPNLGVVRVGEEKSFVIADIPGLIEGA
                     SEGAGLGHDFLRHIERTRVLVHVLDSSGSEGRNPVEDFYKINEELFQYNEKLRDKPQI
                     IVANKTDLPSSQEWIGRIKEEFLPKGHQVLELSAATRDGIQELIYAIWNLLENTETSY
                     ETYDEEYVEVIAPRTDEIIVRKENDRFIVEGDFMERLLGSTYMDDTDSLRYFQEMLRK
                     KGVIDRLKELGITEGESVFICDYEFEFFD"
     gene            1109..1399
                     /gene="yhbY"
                     /locus_tag="VFD79_05270"
     CDS             1109..1399
                     /gene="yhbY"
                     /locus_tag="VFD79_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HZH93493.1"
                     /translation="MLTGKQRSYLKSIAHNLDPIFQLGKNGLTENFVKQVDDALNARE
                     IVKISVLKNSVLDPTDVAVELVQRLNAEFVQSIGRRFVIYRESKDNKTIMLP"
     gene            1459..2064
                     /gene="nadD"
                     /locus_tag="VFD79_05275"
     CDS             1459..2064
                     /gene="nadD"
                     /locus_tag="VFD79_05275"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HZH93494.1"
                     /translation="MAIGLMGGTFNPIHLGHLLMGEYIREEWDLEKVIYIPSGNPPHK
                     TEVDVLDSAYRLRLVELAVKDNPYFQMSDIEFSREGFSYTIDTIRQMSIEYPYERLYF
                     IIGTDTLFELETWKNFQEIAKEIEFIMYGRSNHSEEDIQNKISGLRNDYGFRINRSKG
                     PEIEISSTQIRDRVKRGLSVKYMIPDILIEEIRKESLFKGE"
     gene            2066..2635
                     /gene="yqeK"
                     /locus_tag="VFD79_05280"
     CDS             2066..2635
                     /gene="yqeK"
                     /locus_tag="VFD79_05280"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HZH93495.1"
                     /translation="MDRIIEVLKERIGEKRLNHTLSVVEEALGLARRHKAPEDKVKIA
                     ALLHDFAKNMTLEEALKLAEEYGLELDDVTRTSNELIHGPLGARMVKEYFNIGDQEIL
                     NAITYHTTGRENMTTVEKIVFLADFIEPGREFEGIQVIRERAQEDLDEAILMALNSTM
                     AYLLKKNAIIHVNTVHARNYLLKNRIEEN"
     gene            2637..3500
                     /locus_tag="VFD79_05285"
     CDS             2637..3500
                     /locus_tag="VFD79_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HZH93496.1"
                     /translation="MKKRKNKGMFFRVFLVSFLIFISILFLAGSFLGTNDNHVRFLLL
                     GIDSKDYNQVSSVRSDTIMIVDMNTSSGEINIISIPRDTRATIEGRKSPEKINHSFAY
                     GGPELTLATVRNLLGIELDQYVLADYKLVSDFVDLIGGVEIYVPMDMKYSDPVADPPL
                     HIDLKEGDQTLDGDKALQYLRFRKGYKDADLGRVRAQQDFMKELISQTVRPGNIPKIP
                     GMIGIYSDNMETNIGFGEMLLYGFQSLRLDMDNINSHTLPGSAKMMDGLSYFVHNVDE
                     TEGLLRDIGIK"
     gene            3523..3879
                     /gene="rsfS"
                     /locus_tag="VFD79_05290"
     CDS             3523..3879
                     /gene="rsfS"
                     /locus_tag="VFD79_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HZH93497.1"
                     /translation="MTEINKKLEIIQKAIDDKKGFDIEAMEVGKMTTIADYFVIASGN
                     SSNQVTSIADEVEDKMEQAGFQTLTTKEGYGSARWIILDYEDVIVHLFHRDEREYYNL
                     ERLWSEYEKISKEEKQ"
     gene            3876..4259
                     /locus_tag="VFD79_05295"
     CDS             3876..4259
                     /locus_tag="VFD79_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HZH93498.1"
                     /translation="MNINTLATEKAPAAVGPYSQGVKAGDFVYTSGQLPIDPQTGQLV
                     QEDIQSATRLALNNVKAVLEQGGASLETVVKVTVFVKDMNNFGAINEVYAEFFTDHKP
                     ARSLVEVARLPKDGVIEIEAIAIAK"
     gene            complement(4302..>4492)
                     /locus_tag="VFD79_05300"
     CDS             complement(4302..>4492)
                     /locus_tag="VFD79_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93499.1"
                     /translation="STVSVQVDPLSVMPGKLISKWYLFVFGAYIVMRILILQKKKRRR
                     MSSRRSRQSMYRVPYAPK"
BASE COUNT         1491 a          767 c         1128 g         1106 t
ORIGIN      
        1 taaaagatcc tacgcaggag aaaatggtga agatggacgg gcaaaaaaac aatttggcaa
       61 ggatgggtca gacacatacc ttaaggtacc tgtaggaacc ctcataaagg atgaggagac
      121 tggaaaggtt atagttgacc ttaaggaacc tggtcaggag tttgtcattg caagaggtgg
      181 aaaaggcgga cgtggcaatg cgaaattcgc aacttccaca cgtcaggccc caggatttgc
      241 tgaagcggga acaaagggcc aggaaaggaa aattgtcctg gagcttaagc ttctggctga
      301 tgtgggactg attggatttc caaatgttgg caagtcaaca atactgtcaa cgatttcagc
      361 tgccaagccc aagatagcaa actatcattt caccacacta aaaccaaacc tgggagttgt
      421 aagggttgga gaagagaaaa gctttgtgat tgcggatatt cctggtttga ttgaaggggc
      481 ctcagaaggt gcaggtctgg gacatgactt cctcagacac attgagagaa cgagagtctt
      541 ggttcatgtc ttggattcat ccggcagcga aggtagaaac ccggttgagg atttctataa
      601 aatcaatgaa gagctgtttc aatataatga aaaacttagg gacaagccac agatcatagt
      661 tgcaaacaag accgatttgc cttcatccca ggaatggata gggagaataa aggaagaatt
      721 cttgccaaag ggccatcagg tacttgagct ttcagctgca acaagggatg gaattcagga
      781 attgatttat gcgatttgga atcttctgga gaataccgag acatcatacg agacatatga
      841 tgaagaatat gttgaagtaa tagctcctag aacagatgag atcattgtgc gtaaagagaa
      901 cgatagattt atcgtagagg gagatttcat ggaaagactt ctgggctcca catacatgga
      961 tgatacggat tcccttagat acttccagga gatgctaagg aagaagggtg tcatagacag
     1021 gttgaaggaa cttggaatta ccgagggaga atcagtgttt atctgtgact acgaatttga
     1081 attttttgat taaagaggaa gtgaaaaagt gctaactgga aaacagagaa gttatttaaa
     1141 gagcattgca cataacctgg atccaatatt tcagcttgga aagaatggtt tgactgagaa
     1201 ttttgtcaag caggtggatg atgcactaaa tgcaagagag attgtcaaga taagtgtttt
     1261 gaagaacagt gttttggatc caacggacgt agcagttgag ctggtccaga gattgaacgc
     1321 tgagtttgta caaagtattg ggagaagatt tgtaatatac agagaatcaa aggataacaa
     1381 gacaatcatg cttccctagt tatggagggc ttcagaaaca tctgaagtcc tggatcatct
     1441 gggagaagga ggggtacaat ggctattgga ctaatggggg gaacattcaa ccccattcat
     1501 ttgggtcacc tgctcatggg tgaatacatc agagaggaat gggacctgga gaaggtaatc
     1561 tatatcccat ctggcaatcc accacacaag acagaagtgg atgtcttgga ttctgcctac
     1621 aggttgaggc ttgttgaact ggctgtaaag gataatccat attttcaaat gagtgacatt
     1681 gagtttagca gggaaggatt ttcctacaca attgacacaa taaggcagat gagtatagaa
     1741 tacccttatg agaggctgta tttcataatt ggaacggata ccctctttga gcttgaaacc
     1801 tggaaaaatt tccaggaaat cgccaaggag attgaattca taatgtatgg cagatccaac
     1861 cactcagaag aagatatcca gaataagatc agcggtctta ggaatgatta cggatttaga
     1921 atcaatagaa gtaaaggacc agaaattgaa atttcatcga cacaaattag agatagggtc
     1981 aaaaggggcc tatcggtcaa atatatgatt ccggatattc ttattgagga gattaggaag
     2041 gaatccttat tcaaagggga ataaaatgga caggataata gaggttttaa aagaaagaat
     2101 cggggaaaaa agactcaacc ataccctaag cgtagtggag gaagccctag gcttggccag
     2161 aagacacaag gctccagagg acaaggttaa aatcgcagcc cttttgcatg attttgccaa
     2221 gaacatgacc ttggaggaag ccttaaaact tgctgaggaa tatggtcttg agctggatga
     2281 tgttacaaga acaagcaatg agcttatcca tggtcccttg ggggctagga tggtaaagga
     2341 atacttcaac ataggtgatc aggaaatact aaatgccatt acatatcaca ccacaggaag
     2401 agagaacatg acaactgtgg aaaagattgt ttttttggcg gatttcatag agccaggcag
     2461 ggaatttgaa gggattcagg tcattagaga aagggctcag gaagaccttg atgaagcaat
     2521 actgatggcc cttaacagta caatggcata ccttttaaag aaaaatgcca ttattcatgt
     2581 gaatacggtt catgcgagga attatcttct caagaatcgt atagaggaga attaggatga
     2641 aaaagcgcaa aaataaaggt atgtttttta gagtatttct ggtatccttc cttattttta
     2701 tttccatact ctttctggcg ggcagctttc tggggacaaa tgacaaccac gttagatttc
     2761 tgctcctggg aatagactcc aaggattaca atcaggtatc aagtgtcaga tcagatacaa
     2821 taatgattgt tgatatgaac acatcctctg gtgagataaa catcatctca attccaagag
     2881 atacaagggc aacaattgag ggcagaaaga gtcccgaaaa gataaatcat tcctttgcct
     2941 atggaggacc agagcttaca ctggcaactg tgagaaactt gcttggaata gagcttgacc
     3001 agtatgtact tgctgactac aagttggtaa gtgattttgt agacctgata gggggagtgg
     3061 agatctatgt tcccatggat atgaaatatt ccgatccagt ggccgatccc cctctgcata
     3121 ttgatctcaa ggagggagac cagacccttg acggggataa ggcgcttcag tatttaaggt
     3181 tcagaaaggg ctacaaggat gcagaccttg gaagggtaag ggctcagcag gactttatga
     3241 aggagttgat aagccagact gtaaggccgg ggaacatccc caaaattcca ggaatgattg
     3301 gtatatacag tgacaacatg gaaacaaata ttgggtttgg agagatgcta ctctatggtt
     3361 tccagagctt aagactggac atggacaata taaacagcca tactttgcca ggaagcgcca
     3421 agatgatgga tgggctttca tattttgtcc ataacgtgga tgagacagaa gggctgttaa
     3481 gggatatagg aataaaataa atcaagggag gtctataaaa taatgacgga aataaacaaa
     3541 aagttggaaa taatacaaaa ggctattgac gacaagaagg gttttgatat tgaggccatg
     3601 gaagttggta aaatgaccac catagcagac tattttgtaa tagcaagcgg aaattcatcg
     3661 aatcaggtga cttccattgc tgacgaggtg gaggacaaga tggagcaggc gggattccaa
     3721 accttgacca ccaaagaggg ttacggtagt gccagatgga taatcctgga ctacgaggat
     3781 gtcattgtac atctattcca tagggatgaa agggagtatt acaatcttga aagattgtgg
     3841 tcagaatatg agaaaatttc aaaggaggag aagcaatgaa cattaacaca ctagctactg
     3901 aaaaggcacc tgcagctgtt ggaccttatt cacaaggggt gaaagcagga gattttgtct
     3961 atacatccgg acaattgcca atcgaccctc agacaggcca gcttgtacag gaggacattc
     4021 aatcagctac aagacttgca ttgaacaatg ttaaagcagt actggaacaa ggtggagcat
     4081 ccctggaaac agtggttaag gtaactgtgt ttgtaaagga catgaataac tttggagcta
     4141 taaacgaggt ttatgcagaa ttcttcacag accacaagcc agctagaagc cttgtggagg
     4201 tcgcacgact tccaaaggat ggagtaattg aaatagaagc catagcaata gcaaaatagc
     4261 atgaataaag gccaggacaa tgtgtcctgg ccttttaaca actatttggg tgcataagga
     4321 accctataca tggattgcct gcttcttctg gaagacatcc ttcgccgctt tttcttttgc
     4381 agtatcaata tcctcatgac aatatatgct ccaaatacaa agaggtacca tttgctgatc
     4441 agctttccag gcataactga aagtgggtca acctggactg aaactgtgga ga
//
LOCUS       DASRQF010000197         3560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_497884, whole genome shotgun sequence.
ACCESSION   DASRQF010000197 DASRQF010000000
VERSION     DASRQF010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497884"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VFD79_05305"
     CDS             <1..444
                     /locus_tag="VFD79_05305"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZH93500.1"
                     /translation="LLQLIGAFFWATHILVIGHFASKVNTLKLSSAQFATCSLLSLMV
                     ALVFEEIQMVNIISAAVPILYGGLMSVGVAYTLQVVAQRHAKPSHAAIALSTEAVFAA
                     LGGAIILGEIMSLRGYFGAFLMLAGMLISQGDNILDRSKRKKATS"
     gene            568..750
                     /locus_tag="VFD79_05310"
     CDS             568..750
                     /locus_tag="VFD79_05310"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HZH93501.1"
                     /translation="MMIVVALLIGVAIYYLVTGDNPVGRGPNRGNGPEDKLKERFVQG
                     EISEEEYLRMKEVLRK"
     gene            764..1009
                     /locus_tag="VFD79_05315"
     CDS             764..1009
                     /locus_tag="VFD79_05315"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HZH93502.1"
                     /translation="MMFNRGFYRFGLGNWCGFFGGWDLLIGIGLLLGAIALITWIRSR
                     NSGSREDSYLETLKDRFVRGEITEEEYLSKKNILERK"
     gene            1015..1395
                     /locus_tag="VFD79_05320"
     CDS             1015..1395
                     /locus_tag="VFD79_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93503.1"
                     /translation="MMKKTIAVLLILAVVFTAGGVFMRYSSYAMGFGGYYDDGQQEGF
                     YRGRMPGRGYMNPYWDDEEYRTPVEGDEDVNVWPYRGYQYPNHPMWRYFEDGEYPQWE
                     DFDEDQGYFRGCHGYFYRDEVIEN"
     gene            1412..2098
                     /locus_tag="VFD79_05325"
     CDS             1412..2098
                     /locus_tag="VFD79_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZH93504.1"
                     /translation="MDFSILLVEDQEEISSIVGKYLDKNYYKYDIAKDGFEALELFSG
                     GSYHLAVLDIMLPGIDGFQVLQEIRKTSDIPAIMLTAREQEADRIRGFELGADDYVVK
                     PFSPRELMKRIEVLLKRVYNESNEVVLAVGDLKLYTRSMRLLKGELEVEITSAEYKLL
                     HTFMKNKGLILTRDQLIEKSFGHDYEGYDRTIDTHIKRLRRKIEDDPKNPQYIVTKYG
                     VGYQFGGERA"
     gene            2095..3465
                     /locus_tag="VFD79_05330"
     CDS             2095..3465
                     /locus_tag="VFD79_05330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZH93505.1"
                     /translation="MTIRKLLLIVMVLVVVLSVGINATILTSLTDRYFVDYLDASYEN
                     HTDQIIEYTRAALQREDLSYTQMSRELSSHLIDPIIRIRLLDTDGRQLLDVRADIPMM
                     GGHMMNSRMYERFTSNSAKEFMYQVTSNGELIGNLEITRFGATENTAGALLFKSALIR
                     NSILSALIALVIAIVVGFIISGRMAGELKKTANQATAIQEGNPSPVEKSFIQEITSIR
                     ETLDDLNVRLKLRQKSRKELTDQLIHQTRTPLTILKTHLEAIDDGMVEVDQDEINILE
                     DQIQNITAIISNMSELIDAETTKGEVSLEEVEINQLIRKIMAGLKPQYSKKGVSLNLL
                     TGEKVTLTTDRYMLSQAIYNIMTNAYKYTDEGGKVDISYGIEDGMLGIEISDTGIGIP
                     ERDKEKIFNAYYSAEADGVKVGEGIGLYVAAENLRQLGGRITVQSQVGKGSRFIIELP
                     LDKGRG"
BASE COUNT         1097 a          628 c          958 g          877 t
ORIGIN      
        1 cttttacagc ttattggtgc ctttttctgg gcaacccaca tacttgtgat cggacacttt
       61 gcatcaaagg tcaacaccct taagctttca tcggcccagt ttgccacctg ctccctattg
      121 agccttatgg ttgcgcttgt atttgaggag attcagatgg taaatataat cagtgcggca
      181 gttcccattc tatacggagg actcatgtca gttggtgttg catatacact gcaggttgtg
      241 gctcaaaggc atgcaaagcc atcccatgcg gcaattgcat taagtaccga ggctgtgttt
      301 gcagctcttg gaggagcgat catcttgggt gagattatgt cccttagggg atattttgga
      361 gccttcctta tgcttgcggg aatgctgatc tcacaggggg acaacatatt ggacagaagc
      421 aagaggaaga aggcaacaag ttaatatatt gatgggaaac ggccgcaggg ccgttttttt
      481 atgcaaaaaa atcaatttgt cataatcagg tcacatttgt gttccataat gaagacaaag
      541 atttagatta gacgaggagg aattgagatg atgatagttg tagctttgct tatcggagtg
      601 gccatctatt acctggttac aggggataat cctgttggca ggggaccaaa tagaggaaat
      661 ggacctgagg ataagctaaa ggaaaggttt gtacagggtg aaatcagtga agaggaatat
      721 ttaagaatga aggaagtatt gagaaaataa gggggtgtga atcatgatgt ttaacagggg
      781 attttacaga tttggacttg gaaactggtg tgggtttttt ggaggctggg accttctgat
      841 tgggataggt ctgttacttg gagcaattgc cctaatcact tggataaggt caagaaacag
      901 cggcagcagg gaggattcat atctggagac tttgaaggac agattcgtta gaggtgaaat
      961 aaccgaggaa gagtatctta gcaagaaaaa catattggaa aggaagtaga tgttatgatg
     1021 aaaaagacaa tagcagtttt attgatattg gcggttgtat ttactgcagg aggggtcttt
     1081 atgagatata gctcctatgc catgggattt ggaggctact acgatgacgg tcagcaggag
     1141 ggattctaca ggggcaggat gcctggaagg ggctacatga atccctattg ggatgatgaa
     1201 gagtacagga cgcctgtaga aggtgatgaa gatgtgaatg tatggccata cagggggtat
     1261 caatacccta accatccaat gtggaggtat tttgaggacg gtgaatatcc ccaatgggaa
     1321 gactttgatg aagatcaggg ttatttcagg ggatgtcatg gttacttcta cagggatgaa
     1381 gttatagaga actaggattg agggtgaagt gatggatttt agtatactcc ttgtggagga
     1441 ccaggaggaa atcagctcca tagtagggaa atacctggat aagaactact ataaatatga
     1501 tatagctaag gatggatttg aagcactgga gcttttctcc gggggctcct accatcttgc
     1561 tgttcttgac attatgctgc ctggaatcga tgggttccag gtccttcagg agatcagaaa
     1621 gacctcggat atacctgcca taatgctgac tgcccgagag caggaggctg acaggatcag
     1681 gggatttgag cttggtgcgg atgattatgt tgtcaagccc ttcagcccaa gggagcttat
     1741 gaaaaggata gaggtcctgc tgaagagggt atacaacgaa tccaacgagg ttgtccttgc
     1801 agttggggat cttaaacttt acacaaggag catgaggcta ttaaaggggg aattggaggt
     1861 tgagatcaca tcagcggaat acaagctcct tcataccttt atgaagaaca agggcctaat
     1921 cctcacacga gaccagctca tagaaaaatc ctttggtcac gattatgaag gctatgacag
     1981 gactatcgac acccatataa aaagattgag aagaaagatt gaggatgacc ctaagaatcc
     2041 tcaatatatt gtgacaaagt atggagtggg ctaccagttt ggaggtgaaa gggcatgacc
     2101 ataagaaagc ttctactgat tgtgatggtc cttgtggtgg tgctttccgt tggaataaat
     2161 gcaaccattc tcacctccct tacggacagg tattttgtgg actatcttga tgcaagctac
     2221 gaaaaccaca ctgatcagat cattgagtac acaagggcgg ccctccagag agaggacctg
     2281 tcctacaccc aaatgagcag ggagctgagt tcccacctca tagaccccat aatcaggatc
     2341 aggcttttgg atactgatgg aagacagctc cttgatgtga gagctgatat tccaatgatg
     2401 ggcggacata tgatgaacag cagaatgtat gaaaggttca caagcaattc tgcaaaggag
     2461 ttcatgtacc aggtcacatc caatggtgaa cttattggga accttgagat caccagattt
     2521 ggagctacgg aaaatacagc aggtgctttg ctttttaagt cagccctgat caggaacagt
     2581 attctttctg ccctgatagc attggttatt gcaattgtag ttggttttat aataagtgga
     2641 cgcatggcag gagagcttaa aaagacagcc aaccaggcca cagccataca ggaggggaac
     2701 ccgtcacctg ttgagaaatc ctttatccag gagataacca gcatacggga gaccctggat
     2761 gacctgaatg tgaggcttaa gcttaggcaa aagagcagga aggagcttac agaccagctg
     2821 atccaccaga ccagaacacc cttgaccata ctgaagaccc atctggaggc aatagatgat
     2881 gggatggtgg aggtggacca ggacgagatc aatatcctgg aggatcagat acaaaacata
     2941 actgccataa tatccaacat gagcgaactg attgatgctg aaaccacaaa gggtgaagta
     3001 agccttgaag aggtggagat aaatcagctg atcagaaaaa tcatggctgg actcaagccc
     3061 cagtattcaa aaaaaggagt tagcctaaac cttctcacag gagaaaaggt gaccctaaca
     3121 acagacagat atatgctcag tcaggctatc tacaatataa tgacaaatgc ctataagtac
     3181 accgatgaag gtggcaaggt tgatatcagt tatggaatag aggatggaat gctgggcatt
     3241 gaaataagtg acactggaat tggaatcccg gaaagggaca aggaaaagat attcaatgcc
     3301 tactacagcg cagaagcaga tggcgtaaag gttggagagg gtatcggact ctatgttgca
     3361 gcagagaatc tgagacagct tgggggaaga ataactgtcc aatctcaggt tggtaaagga
     3421 agtaggttca taattgaact gccactagat aaagggaggg gttagatgaa gaagcttaag
     3481 aaaaagttga atgaattcat agagaagctt gcaaaggcaa atgaaagtga gtttggaaac
     3541 aagaagcttg attgctgcca
//
LOCUS       DASRQF010000198         2993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1513831, whole genome shotgun sequence.
ACCESSION   DASRQF010000198 DASRQF010000000
VERSION     DASRQF010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513831"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2229
                     /locus_tag="VFD79_05335"
     CDS             <1..2229
                     /locus_tag="VFD79_05335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZH93506.1"
                     /translation="FQAEEDQGLHFAVRKDWPQLVSILDKAVGSISQEERIAINNRWI
                     GLDIHVDYSGIIRVVAMIGLFMAAILGVSFYWNIKLRSEITVRKQVQEDLEKAKREAE
                     EANEFKSSFLARMSHEIRTPLNAITGMSYLLKKTGLSPTQKTYTERITQASNNMLSIV
                     NDILDYSKIESGKVELETVSFNLDRVIQDVVSIATYKIEEQGIGFRLSKDPQLANWYF
                     GDSKRIEQVLLNLLNNSAKFTNSGEVSLDIKLVAKENDTCHIAFTIRDTGIGMSRDQV
                     DNLFMPFVQGDTSINRRFGGSGLGLSIVKNLVDMMDGKIQVYSTQGEGSTFIVNLSLK
                     VDEESESEYLKVFSARHFEDIKSLILEKSGTNMNLIESYLGSFGMECEITSSPDSAVR
                     MLEAPDGVFSKAYDLFIVDYDSTTNDAFLFVEELRRNRSLAKMPRIIMLLPMMRDDLF
                     DLVQERNIDIGIGKPIIPSVLLDGILDTLNIIPDESGEKISMAKTEVGMNSGCVLVVE
                     DNTTNQMIARSLLDQVGIETITALNGQEGVEAFRANRDRIDLILMDLHMPLMNGYDAA
                     VEIRKESGDVPIIAMTADVVLGVEEKCRESGIDGYISKPFDPDMFIEMVQTYINRGQV
                     DVQDEETYLDTSAGIRNMGGSSDLYHKVLGQYRDENMETLSLFNDMINSEDYREAAGL
                     MHKLKSSSATIGATRLYDLAKDLQRALEEEKVEIVERIGNEFSLVFENVMKEIDKRLK
                     SL"
     gene            2258..>2993
                     /locus_tag="VFD79_05340"
     CDS             2258..>2993
                     /locus_tag="VFD79_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZH93507.1"
                     /translation="MAAKILIVDDSATDRMIIQSMLSEYETILANDGLEALAVIEREP
                     DIDLVILDLNMPNMDGFQVIQAVKEEKEKSNLRIIILTNYDELENEVKGLLMGAVDYI
                     RKPIQMETLKLRIEIHLKQREILNAIESNLEEKSVTFDSLFNNAPIGITISYGGSSDD
                     DNMEHALINPTFEKITGRSKEDLLNLGWAEITHPEDLKEELPKYRSFVNGEIEGYSMD
                     KRYIKKDGSPVWVHIVVSRLSPSNGGK"
BASE COUNT          938 a          561 c          757 g          737 t
ORIGIN      
        1 tttcaggctg aggaggacca gggtcttcac tttgcggtca ggaaggattg gccccaactt
       61 gtaagcatct tggacaaggc tgtagggtcc atttcccagg aggaaaggat tgcaatcaac
      121 aacaggtgga tcgggcttga catccatgtt gactacagtg gcataatccg cgttgttgca
      181 atgataggtc tttttatggc tgcaattctg ggggtttcct tctattggaa catcaagctc
      241 agaagtgaga tcaccgtcag gaaacaggtc caggaagacc tggaaaaggc taagagggaa
      301 gcggaggagg ccaatgagtt caagtcaagc ttccttgcaa ggatgtccca tgaaatcagg
      361 acccccctaa atgcaataac ggggatgtca tatctcctga aaaagacagg cctgtcacca
      421 actcagaaaa cctacacaga aagaattacc caggcctcta acaatatgct gagcatagtc
      481 aatgatattc tggattattc caagattgag tctggaaagg ttgagcttga gactgtatcc
      541 ttcaatttgg acagggtaat ccaggatgtg gtgagcattg ccacatacaa gatagaggag
      601 cagggaatag gattcaggct atcaaaggat ccacagcttg caaactggta ctttggagat
      661 tccaagagga ttgagcaggt ccttctgaac cttttgaaca actctgccaa gtttacaaat
      721 agcggggagg tttcccttga cataaagctt gtggcaaagg agaatgacac ctgccacatt
      781 gcatttacaa tacgagacac tggcatcgga atgagccggg accaggttga taatctgttc
      841 atgccatttg ttcagggaga cacctccata aacaggaggt ttggagggtc aggccttgga
      901 ctttccatag tgaaaaacct tgtggacatg atggatggga aaatccaggt ctacagcact
      961 cagggagagg gatcaacctt tattgtgaac ctttccctta aggtggatga ggaaagtgaa
     1021 agtgaatacc tcaaggtatt ttcagccaga cattttgagg acataaagag cttgatcctt
     1081 gagaaatcgg gaaccaatat gaaccttatt gaatcctacc tgggctcctt tggcatggaa
     1141 tgtgaaataa ccagttcccc agacagtgct gtaaggatgc ttgaagctcc tgatggggta
     1201 ttctcaaagg catatgacct tttcatagtc gattatgact caacaacaaa tgatgccttc
     1261 ctctttgtgg aggaactgag aagaaacaga agtcttgcca agatgcccag gataataatg
     1321 ctcctgccca tgatgaggga tgacctcttt gacctggttc aggaaaggaa tatagacata
     1381 ggaataggta agcccataat cccatctgtt cttctagatg gaattctcga caccttgaat
     1441 attataccgg atgagtctgg tgagaaaatc tccatggcaa agaccgaggt tggaatgaat
     1501 tcaggctgtg tacttgttgt tgaggataat accacaaacc agatgattgc aagaagtctt
     1561 ctggaccagg tggggataga gacaatcaca gccctgaatg gacaggaggg tgttgaagcc
     1621 ttcagggcca accgtgacag gatagatttg attctgatgg acctccatat gccccttatg
     1681 aacggatatg atgcagcagt tgaaatcagg aaggaatctg gggatgtacc aataattgcc
     1741 atgactgcag atgtggttct aggtgtagag gaaaaatgca gggagagtgg aattgatgga
     1801 tatatcagca agccctttga ccctgacatg ttcattgaga tggtccagac ctatatcaat
     1861 cggggccagg tggatgtaca agatgaagaa acttacctgg atacttcagc tggcatcagg
     1921 aacatgggtg gaagcagcga tctgtaccat aaggttttag ggcagtatag ggatgaaaac
     1981 atggagactt tatcattgtt taatgatatg attaatagtg aggactacag ggaagcagcc
     2041 ggtcttatgc acaagcttaa gagcagctct gctaccatcg gtgcaacaag gctttatgac
     2101 ctggccaaag accttcagag ggccttggaa gaggaaaaag tggaaatagt tgagagaatt
     2161 ggaaatgagt tttcacttgt ctttgagaat gtaatgaagg aaattgataa aaggcttaag
     2221 agcctataaa gatggcttaa tcacaagggg gatgtttatg gctgcaaaaa tacttattgt
     2281 ggatgattct gcaacggacc ggatgataat tcaaagtatg ctttcagaat atgaaaccat
     2341 cttggcaaat gatggtttgg aggcacttgc cgtcattgaa agggaaccgg atatcgatct
     2401 tgtgatcctt gaccttaaca tgcccaatat ggatggcttc caggtgattc aagctgtaaa
     2461 ggaagaaaag gaaaagagta acctaaggat aataatcctg acaaactatg atgaacttga
     2521 aaacgaggtc aagggcctcc ttatgggagc agttgactac atcagaaagc ccatccagat
     2581 ggagaccctt aagctaagaa tagagattca tctgaaacaa agagaaatcc taaatgcaat
     2641 tgaatcaaac cttgaagaaa aaagtgttac ctttgactcc ctctttaaca atgctcccat
     2701 aggaattaca atttcctatg gtggttcaag tgatgatgac aacatggagc atgctttgat
     2761 aaatccaacc tttgaaaaaa taacaggaag atcaaaggaa gaccttctga accttggctg
     2821 ggctgaaata acccatccgg aggatctgaa ggaagagctc ccaaaataca gaagctttgt
     2881 aaatggtgaa attgaagggt attcaatgga caagcgctac atcaagaagg atggttcacc
     2941 agtgtgggtc catattgtcg tatcaaggtt aagtccatca aatgggggaa agt
//
LOCUS       DASRQF010000199         6378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_918780, whole genome shotgun sequence.
ACCESSION   DASRQF010000199 DASRQF010000000
VERSION     DASRQF010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6378
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918780"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="VFD79_05345"
     CDS             <1..1077
                     /locus_tag="VFD79_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HZH93508.1"
                     /translation="NPVKTQLSTVGKGDKVDLLKRAYHSAKEFDQVISQVTTAYMEEE
                     QKVFIANSDGLWTRDDRTRVRLAVSATAGLNGELQQGYFAPGAAMGFEFFDYVDVAKV
                     GAEAARIAKAMIHAKYIPGGKMPVVIENGFGGVLFHEACGHALEASFVSKGTSAFGGK
                     IGQEVASSFVTAIDDGTIANGWGTTSIDDEGTPTRKNVLIQNGILKSYLIDKLNGRRM
                     GLESTGSARRQSYRYPPTSRMNNTYIAPGHMTLNDMIGPIEFGLYAKKLGGGSVNPAT
                     GDFNFSVMEGYLIKNGEIADPVRGATLVGNGIEVLRLIDGVGSNLKMGQGMCGAASGS
                     IPVNVGQPAIRVSSITVGGRRGND"
     gene            1077..2426
                     /locus_tag="VFD79_05350"
     CDS             1077..2426
                     /locus_tag="VFD79_05350"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HZH93509.1"
                     /translation="MDFDAFSSLVFDHGKGRLDEMELFYTSRRALRIEVFNQKVDNYI
                     VSDTAGVALRAVGSLGMGQSFTEKMDERSAELLVEEAYQNGQFTDADERPNFAKANEA
                     RGSRHNISERLENIQTNEKIQMMINLETEALSLDSRVSKIQMCTYEDNLTERKIRNSL
                     GLSLEDSSSLGYVYISVVAEQEDDVSTGSSFAVFRDPGEIDISEIAMEAVEEATSLLG
                     AKPISSGNYGAIIKNRAFVDIIEGFMPAFSGLNVQRGLSFLKGRIGEVIGSPKITIIE
                     DPFLQDGFASCDFDDEGFRTTRKTIIHEGRLKEFLYDSRSSKKEVNKTSGNGFRDSIK
                     STVGTRPTNLLVEKGSRELVEIIEEMDSGILITDLAGLHSGLDFVSGDFSLQAQGFTV
                     ESGLIGEPVKGITISGNMIQLLAGIEEVADDIFFGLPGNGCYGSPSIKIDSISVSGY"
     gene            2708..3241
                     /gene="nuoE"
                     /locus_tag="VFD79_05355"
     CDS             2708..3241
                     /gene="nuoE"
                     /locus_tag="VFD79_05355"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HZH93510.1"
                     /translation="MDCCGAKELKGHFLDEEVDLSLMDETLKKYKEVKGSLITILQNA
                     QEIYGFLPMSVLEYISQETGIYSSQIYGVATFYTQFRLNPVGENLIMLCKGTACHVNG
                     ADNIEEAITEELGIKDGETTEDKVFTLNNVACLGCCSLSPVMMINGETYGSLTAQKTK
                     EILREIGKQTLEVKEGN"
     gene            3242..4987
                     /locus_tag="VFD79_05360"
     CDS             3242..4987
                     /locus_tag="VFD79_05360"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HZH93511.1"
                     /translation="MKVVVGLGSCGIAAGARKVYEELENQVNNMGMDIDLEITGCVGM
                     CHLEPIVDIYDDEGRMERFVKVSGDKIAELVEKKLAKEEDNTYRIFSVDEEALGKQVR
                     IAIENCGIINPERLEEYIEKDGYKALEKCLKEMSQKSVIDEIRDSGLRGRGGAGFPTW
                     FKWNAAMQEKNTPKFMVCNADEGDPGAFMDRSILEGDPHRLLEGMAIGAYAIGAEEGI
                     IYVRAEYPLAIKRLDIAIKQARDAGYLGKDIMGTGFNFDIRIKAGAGAFVCGEETALI
                     ASLEGERGMPRLKPPFPAQKGYWGQPTNINNVETFANVPWIIRKGGDAFAAYGTAQSK
                     GTKVFALTGKIKKGGLVEVPMGMPIKEIIYGIGGGILNDKEFKAVHMSGPSGDCIPAF
                     ISDTTVDYESLGKVGAIMGSGGMVVMDESTCMVDMARFFLDFTRKESCGKCIHCRLGT
                     KRMLEILERIVKGDGREGDIELLESLAVEVKDGSLCGLGQTAPNPVLSTIRYFRNEYE
                     AHINEKKCPAKSCKALITYEIQDNCIGCGMCKRNCPVEAISGEPKLLHEIDQEICIQC
                     GKCYEVCKFDAITIS"
     gene            5003..>6378
                     /locus_tag="VFD79_05365"
     CDS             5003..>6378
                     /locus_tag="VFD79_05365"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZH93512.1"
                     /translation="MSVIHLNINGKEVRGNAGQTILDIARDNKIDIPTMCFDERVEIY
                     GSCGMCVVEVEGVPKLLRSCATTASDGMIINTNTERVKSSRKTALELLLSDHVGDCKA
                     PCVLACPGYTDCQGYVGLAANMEYREGLKLIKEQLPLPACIGRVCPHPCEDACRRQLV
                     EEPISIAYLKSFLADIDLASDNVFIPELGEPTGKKIAIVGGGPGGLSAAYFLRVAGHE
                     VTIYEQMPEMGGMLRYGIPEYRLPGSVIDQEVAIVEKMGTRFVNNVKVGEQLDLSYIQ
                     NNFDACYISVGAWKSTPLRLEGEDLPGVIGGIDFLRKATVNEDMRIGERVAVVGGGNT
                     AMDACRTAVRLGAKEVYVLYRRTEAEMPAELIEIKEAKEEGVQFKFLVSPLEIIEENG
                     RAAKVKLQKMELGEEDASGRRRPVPIEGEIEYIDVDLVIGAIGQQSALEGFEILEKTK
                     RGTIAVDE"
BASE COUNT         2029 a         1055 c         1712 g         1582 t
ORIGIN      
        1 aacccagtta agactcagtt atcaactgtt ggaaaagggg acaaggtaga tcttctaaag
       61 agggcttacc attcagccaa ggaatttgat caagtcatct cacaggttac aactgcctac
      121 atggaggaag aacagaaggt cttcattgca aattccgatg ggttatggac aagggatgat
      181 aggaccagag taagacttgc ggtttctgca acagctggtt taaatgggga gcttcaacag
      241 ggttactttg cacctggagc tgcaatgggc tttgaattct ttgattatgt tgatgttgca
      301 aaggttggag cggaggcggc taggattgcc aaagcaatga ttcatgcaaa gtatatacct
      361 ggaggtaaaa tgcctgtggt aattgaaaat ggatttggcg gagttctctt tcatgaagcc
      421 tgtggccatg cattggaggc ttcctttgtg tccaagggaa cctcggcatt tggagggaag
      481 attggccagg aggttgcatc tagttttgtc actgctatag atgatgggac aatagccaat
      541 ggttggggta caacaagcat tgatgacgaa gggacgccga ccaggaaaaa cgtacttatt
      601 cagaatggaa tacttaaatc ctacctgatt gacaagctca atggtagaag aatgggtttg
      661 gaatcaactg gatcagcacg tcgacaatca tacagatatc ctccaacctc aaggatgaac
      721 aatacctata tagcacctgg tcacatgaca ttgaatgata tgattggacc aatagaattt
      781 ggactctacg caaagaaatt gggcggtgga agtgtaaatc cagcaactgg tgacttcaat
      841 ttttcggtca tggagggcta tctcataaag aatggggaaa tagctgatcc tgttaggggt
      901 gcaacactgg ttggcaatgg gattgaggtc ttgaggctaa ttgacggggt tggtagcaac
      961 ctgaaaatgg gacaaggaat gtgtggtgca gcaagtggct cgattcctgt caacgtaggt
     1021 caacctgcaa tcagggtaag cagcatcact gttggtggta gaagggggaa tgactaatgg
     1081 attttgacgc ttttagcagc ctggtttttg accatggaaa aggcaggctg gatgaaatgg
     1141 agctcttcta cacttcacgt agggccttaa gaattgaggt attcaatcaa aaggttgaca
     1201 actacattgt atccgatact gctggagtgg ctctaagggc agtgggatca ctcggaatgg
     1261 gacaatcctt tacggagaaa atggatgaaa ggtcggctga attgctggtt gaagaggcgt
     1321 atcaaaatgg acagttcaca gatgcagatg aaagaccaaa ttttgcaaaa gccaacgaag
     1381 ctagaggaag tagacataat atctctgaaa ggcttgaaaa tatacagaca aatgaaaaga
     1441 tacaaatgat gattaatctt gagaccgaag ccttaagcct tgacagtagg gtcagcaaga
     1501 tccagatgtg cacttatgaa gataatctta cagaacggaa gataagaaat tcactgggcc
     1561 tgagccttga ggacagttca tccctaggtt atgtgtacat ttcagtggtg gcggaacagg
     1621 aagatgatgt tagtacaggc agctcatttg cagtattcag ggatccaggg gaaatagaca
     1681 tttctgaaat tgcaatggag gctgttgaag aagccacttc acttttaggt gcaaagccaa
     1741 taagtagtgg aaattatggt gccattatca agaacagggc atttgtcgat attattgaag
     1801 gttttatgcc agcattttca ggattgaacg tacaaagggg cttgtctttc ttaaagggaa
     1861 gaattggcga ggtcataggc agtccaaaga taaccataat agaagaccct tttctccagg
     1921 atggatttgc cagttgtgat tttgacgatg aagggttcag gactacacga aagaccataa
     1981 tccacgaagg gagattaaag gaatttctct acgattccag atcatcaaaa aaggaagtca
     2041 acaaaaccag tggaaatgga tttagagatt cgataaagtc aacagtggga acaaggccaa
     2101 ccaatctgtt ggtggagaag ggttccagag agctggtaga aataatcgaa gaaatggatt
     2161 caggtatctt gattactgac ctggcagggc tccactctgg attggatttt gtgtctggag
     2221 atttttccct gcaagcccaa ggtttcactg ttgaaagtgg attaattgga gaacctgtca
     2281 agggcattac catatcagga aatatgattc aactacttgc tggtatagaa gaagtagctg
     2341 atgatatatt ctttggacta cctggtaatg gctgctatgg ttcaccctct ataaaaattg
     2401 attcaataag tgtttctgga tattgatagt ttgataacat gatgcaacaa aggtataaat
     2461 gttagtattt gaatccatgg taagggaaga ttagctgggg aatctgcttc tatgggcagg
     2521 gccccctttt tttgaaagag taaaatatgt tcattttggg ggacttgaaa tcagtttagg
     2581 catgaataaa catgtgaata ccataacgat aatagtggga ttttctggcc tcaactgatt
     2641 gttaattctg aatatttaag gtataataaa tttattgact taaattacaa gtaaaggagt
     2701 tggtaacgtg gattgctgtg gagcaaagga actaaaagga cacttccttg atgaagaagt
     2761 ggatttgtca ctgatggatg aaacactgaa aaaatataag gaagtaaaag gtagcctgat
     2821 tacaatactt caaaatgccc aggaaatata tggattttta cccatgtctg tactggaata
     2881 catatctcag gagaccggaa tttacagctc acagatatat ggtgtagcaa ccttctacac
     2941 tcagttcaga ctaaacccgg ttggggagaa tctgatcatg ctgtgtaagg gtactgcttg
     3001 tcatgtaaac ggagcagata atatcgaaga ggcaattact gaagagttgg gcatcaagga
     3061 tggagagacc acagaggaca aggtgttcac attgaataac gtggcctgtc tcgggtgttg
     3121 cagcctgtca ccggttatga tgatcaatgg ggagacatat ggtagtttga cagctcagaa
     3181 aaccaaggaa atcttaaggg aaatcggcaa gcagaccctt gaagtaaagg aggggaacta
     3241 gatgaaggta gtggttggtc tgggaagttg cggaatagcg gctggcgcca ggaaggtata
     3301 tgaagagctg gagaatcagg taaacaacat gggtatggac atagatttgg agatcaccgg
     3361 ctgtgtcggc atgtgtcatc tggaacccat agttgacata tacgacgatg aaggaagaat
     3421 ggagcgcttc gtaaaagttt caggagataa aattgctgag cttgttgaaa aaaaactggc
     3481 aaaagaagaa gacaatacat ataggatttt ttcagttgat gaggaagcac tggggaaaca
     3541 ggtaagaata gcaattgaaa actgtggcat catcaatcct gaaagattgg aagaatacat
     3601 agaaaaagat ggttacaagg ccttggaaaa gtgcctgaag gaaatgtcac aaaaaagtgt
     3661 catagatgaa ataagggact ccggattaag aggaagaggt ggagcaggct tcccaacctg
     3721 gtttaagtgg aatgcagcca tgcaggaaaa gaacactcca aaattcatgg tatgtaacgc
     3781 tgacgaagga gacccgggag catttatgga caggtccata cttgaaggag acccacacag
     3841 acttttggag ggtatggcaa taggagccta tgccatcgga gcagaagagg gtatcattta
     3901 cgttagagct gagtatccac tggccataaa aagacttgat attgcaatca agcaggcgag
     3961 ggatgctgga taccttggca aggacatcat gggaacaggc tttaatttcg acataagaat
     4021 aaaggcaggg gctggagctt ttgtatgcgg tgaggaaaca gccttgatag cttcccttga
     4081 gggagaaaga gggatgccca gactaaagcc gccattcccg gcacaaaagg gatactgggg
     4141 tcagccaaca aacataaaca acgttgagac cttcgcaaat gtcccatgga taataagaaa
     4201 aggtggagat gcctttgcag catatggtac agcacagtcc aagggaacca aggtattcgc
     4261 attgaccggt aaaataaaaa aaggtggact tgttgaggtg cctatgggca tgcccataaa
     4321 ggaaatcata tacggaatcg gtggaggaat acttaacgac aaggagttta aggctgtaca
     4381 tatgagcgga ccatctggtg actgtattcc agcgtttatt tcagatacaa cagtggacta
     4441 tgagtcactg ggcaaggttg gagccatcat gggttctggt ggtatggttg taatggacga
     4501 gtcaacctgt atggttgaca tggcgagatt cttccttgac ttcactagaa aggaatcctg
     4561 tggaaagtgt atccattgca gactgggaac caagagaatg cttgaaattc tggagagaat
     4621 tgtaaagggt gatggcaggg aaggcgatat tgagttactt gagagcctag ctgtggaggt
     4681 taaggatggt tcactttgcg ggcttggcca aacagcacct aatccagtac taagtacaat
     4741 tagatatttc agaaatgaat atgaagcaca cataaatgag aaaaaatgtc cggcaaaatc
     4801 atgtaaagcg cttattacct atgaaattca ggataactgc attggttgtg gaatgtgtaa
     4861 gagaaattgt ccggttgaag ccatatcagg tgagcctaag cttcttcatg aaatagacca
     4921 ggaaatatgt atccagtgtg gaaagtgcta tgaggtttgt aagtttgacg caataacaat
     4981 aagttaaagg taggggtgaa atatgtctgt tatacacttg aacataaatg gaaaagaagt
     5041 aagaggtaat gcaggtcaga cgatacttga cattgcaaga gataacaaaa ttgatatccc
     5101 taccatgtgc tttgatgaaa gggtagagat atatggatca tgcggaatgt gtgtggttga
     5161 ggttgaaggg gttccaaaac ttctaaggtc atgtgccaca actgcttccg atggaatgat
     5221 aataaataca aataccgaga gggtcaaaag ctctagaaag acagctttgg agctgcttct
     5281 ttcagaccat gtgggtgatt gtaaggcacc gtgtgttctg gcttgtccgg gatatacaga
     5341 ttgccaggga tatgtgggac ttgcagccaa catggaatac agggaaggac ttaagctgat
     5401 taaggagcaa ttgccacttc cagcatgtat aggaagggtt tgtccccatc cctgtgagga
     5461 tgcatgtaga agacagctag ttgaggaacc tatttccata gcatacctta agtcattcct
     5521 tgccgatata gacctggcaa gcgacaatgt attcatacct gaactgggag agcccacagg
     5581 gaagaaaatt gccattgtag gcggaggccc aggtggattg tcagcagcct acttccttag
     5641 ggtcgctggt catgaggtaa ccatctatga gcagatgcct gaaatgggcg gaatgcttag
     5701 atacggaatt cctgagtaca gacttccagg aagtgtcatt gaccaggaag ttgccatagt
     5761 tgaaaaaatg ggaacaagat ttgtaaacaa tgtaaaggta ggagaacagc ttgacctttc
     5821 ctacattcag aataacttcg atgcatgcta catatctgtt ggagcctgga agagtacacc
     5881 attaagactt gaaggggaag accttcctgg agtgattggt ggaattgact tcctaagaaa
     5941 ggctacagtg aatgaagaca tgagaatagg ggaaagagtt gcagtagtag gaggcggaaa
     6001 taccgctatg gatgcctgcc gtactgctgt aagacttgga gccaaggaag tttatgtgct
     6061 ctacagaaga acagaggctg aaatgccagc tgaactaata gagatcaagg aagccaagga
     6121 ggaaggggta cagtttaagt tcctggtttc accacttgag atcatagagg agaatggaag
     6181 ggcggccaag gttaagctgc agaagatgga gcttggtgaa gaagatgcct ccggaagaag
     6241 aagacctgtt ccaattgagg gagaaattga atatatcgat gttgatttgg taatcggagc
     6301 aattggtcag caatctgcac tggagggctt cgagatactt gagaaaacca agagaggcac
     6361 cattgcagtt gatgaaag
//
LOCUS       DASRQF010000200         2359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_134420, whole genome shotgun sequence.
ACCESSION   DASRQF010000200 DASRQF010000000
VERSION     DASRQF010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134420"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /gene="metG"
                     /locus_tag="VFD79_05370"
     CDS             <1..367
                     /gene="metG"
                     /locus_tag="VFD79_05370"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HZH93513.1"
                     /translation="KPEEKPVEEKKEEEEITIDDFAKVKFKVAKVIACEDHPKADKLL
                     VLTLKVGDEERQVVSGIKQWYAPEDLIGKKVIVVANLKPVKLRGVESRGMILAAQDDN
                     GKLTLLSTLEDIDEGSVVS"
     gene            378..1160
                     /locus_tag="VFD79_05375"
     CDS             378..1160
                     /locus_tag="VFD79_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HZH93514.1"
                     /translation="MLIDSHAHLDDRRFDPDRETLIESLKDNGIDMVINVGADVGSSR
                     AAVDLAKKYENIYAVVGVHPHSAKDLEGKDLSEIREMLREDKVVAIGEIGLDFYYDNS
                     PRDIQRKWFKAQIDLAKELDLPIVIHTRDAAQETFDILKEAAGDGRLKCLLHCYSGSA
                     EMAEEYLKLGFFISLGGSVTFKNARVSREVARVVPLDRLLIETDSPYLTPEPYRGKRN
                     EPMYVKYVAERIAQEKGVTFEEVAQATSDNVYRFFGIQRKGQ"
     gene            1166..1711
                     /gene="rnmV"
                     /locus_tag="VFD79_05380"
     CDS             1166..1711
                     /gene="rnmV"
                     /locus_tag="VFD79_05380"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HZH93515.1"
                     /translation="MIKEVIVVEGKDDISAVKAALQCEVIATNGFGYGRKLIETLKSI
                     HKRRGIIILTDPDYGGERIRRDISKHIPDCKHAFLPQGKALKKDNIGIENASPEDIRE
                     AIEKARPNRVEAKVNFQRDDLFLFNLAGGPGSKERREKLGEILGIGYTNGKQLLNRLN
                     NLGVSREEFLKAMERIDKDGQ"
     gene            1701..>2359
                     /gene="rsmA"
                     /locus_tag="VFD79_05385"
     CDS             1701..>2359
                     /gene="rsmA"
                     /locus_tag="VFD79_05385"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HZH93516.1"
                     /translation="MDNKRLYSPSQVREVLDRHGFTFSKGLGQNFLIDGNIVRKIVRS
                     ADIGKDDYVLEIGPGMGTLTEELALNAKKVIALEIDERLRPVLAETLDPYDNVEVVYG
                     DVLKLPLREIIEEKCGGQPVKVVANLPYYVTTPIIGRLIEENLPLKSISVMVQKEVAD
                     RMVAKPHTKDYGSLTLFVGFYTEPQIMMKVPKTVFMPQPKIDSSVIRLNVKKDLPDTN
                     R"
BASE COUNT          716 a          507 c          648 g          488 t
ORIGIN      
        1 caagcctgaa gaaaagccag tggaggagaa aaaagaggaa gaggagatca caatcgatga
       61 ctttgccaag gtaaagttca aggttgccaa ggttattgcc tgtgaagacc atcccaaggc
      121 cgacaagctc cttgtcctga cccttaaggt tggggatgag gaaagacagg ttgtatcagg
      181 aataaagcag tggtacgcac ctgaggacct aattggcaag aaggtaatag ttgttgcaaa
      241 cctaaaacct gttaagctta ggggggttga atccagggga atgatactgg cagcccagga
      301 tgataacgga aagcttacgc ttctttcaac ccttgaggat atagatgaag gatcggtagt
      361 atcataggag gtaatttatg ttgatagaca gtcatgccca tctggatgac cgcaggtttg
      421 atcctgacag ggagacacta atagagtccc ttaaggacaa tggaatagac atggtcataa
      481 atgtgggagc tgatgtaggg tccagcaggg cagctgtgga ccttgcgaaa aaatatgaga
      541 acatctatgc cgttgtgggg gttcatcccc actcggcaaa ggatttggaa ggcaaggacc
      601 taagtgagat aagggagatg ctaagggagg acaaggttgt ggccattgga gaaatcggcc
      661 ttgacttcta ctacgacaac tccccaaggg acatacagag gaagtggttc aaggcccaga
      721 ttgaccttgc caaggaattg gaccttccaa tagtgatcca cacaagggat gcagcccagg
      781 agacctttga tatccttaag gaggctgcag gggatggcag gctcaagtgt ctcctccact
      841 gctattcagg cagtgcggag atggctgagg aatacctgaa gcttggattt ttcatttccc
      901 tgggaggttc tgtcaccttc aagaacgcca gggtatcccg tgaggttgca agggttgttc
      961 cccttgacag gctccttatt gaaaccgaca gcccctacct taccccggaa ccctacaggg
     1021 gaaagagaaa cgagcccatg tatgtaaagt atgtggcaga gaggattgcc caggaaaagg
     1081 gagtgacctt tgaagaggta gcccaggcaa caagtgataa tgtttacagg ttcttcggaa
     1141 tccaaagaaa gggtcagtga aggctatgat caaggaagtt atagtagttg aaggcaaaga
     1201 tgacatctcc gcagtcaagg ctgccctcca gtgcgaggtg attgcaacaa atggctttgg
     1261 atacggaaga aagcttattg aaaccctaaa atccatccac aagaggaggg ggatcatcat
     1321 actgacagat cctgactacg gtggagaacg gataagaagg gacatatcaa agcatattcc
     1381 agattgcaag catgccttcc ttccccaggg caaggccctt aagaaggaca atattggaat
     1441 tgagaacgca agccccgagg atataaggga ggccatagaa aaggccagac caaacagggt
     1501 ggaggccaag gtcaacttcc agagggatga cctctttctc tttaaccttg caggtggacc
     1561 cggctcaaag gaacgaaggg aaaagcttgg ggaaatcctg ggaattggat acaccaacgg
     1621 aaagcagctc ttgaacaggt tgaacaatct gggagtgtcc agggaggagt ttctaaaggc
     1681 catggaaagg atagacaagg atggacaata aaagactgta ttccccaagc caggtaaggg
     1741 aggttctgga ccgtcacggc ttcaccttct ccaagggcct gggacagaat ttcctaatag
     1801 acggaaacat cgtaagaaaa atcgtaagaa gcgcagatat cggcaaggat gactatgtcc
     1861 ttgaaatagg cccaggcatg ggaaccctta cagaggagct tgcccttaat gcaaagaagg
     1921 tcatagccct ggagatagat gaaaggctaa ggcctgtcct agctgaaacc ctggacccct
     1981 acgacaatgt tgaggttgtc tatggggatg tattgaagct gccactaagg gagatcatag
     2041 aggaaaaatg cggtggccag cctgtgaagg ttgttgccaa ccttccatat tatgtcacaa
     2101 ctccaataat cggaaggctc attgaggaaa acctacccct caagtccatc agcgtaatgg
     2161 tacagaagga ggttgccgac cggatggttg ccaagcccca caccaaggac tacgggtccc
     2221 taaccctctt tgttggcttc tacacagagc cccagataat gatgaaggtt ccaaagaccg
     2281 tcttcatgcc ccagcctaag atcgacagct cggtcataag gctcaatgta aaaaaagacc
     2341 ttcccgacac caacaggga
//
LOCUS       DASRQF010000201         2222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1145505, whole genome shotgun sequence.
ACCESSION   DASRQF010000201 DASRQF010000000
VERSION     DASRQF010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145505"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="VFD79_05390"
     CDS             <1..860
                     /locus_tag="VFD79_05390"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZH93517.1"
                     /translation="REDTSLLLFNIGVILTELGKLDGAIRCYKKIIEINPIYVDAYYQ
                     LGVISEDKGEYEEAVELYKSALRIDSKDTLSMYNLANLYDLKEEEGRARNLYKRILKL
                     EPDHPYTLNNLGAILESKERYNEAYELIKRSIDSKDDFFMSHFNMGVVLKALGNPEKA
                     LWHYKRSIELNANYHYSYLNMSAIYIEGGQYHEAIDILTMGIGANPTAHDLYYNRACS
                     LAILGSDQKALDDIERALGMHQQLIKWVEWDKDFDSIRGKARFREIIGKYNMTKGKEE
                     ADDNGQNQR"
     gene            835..1593
                     /gene="map"
                     /locus_tag="VFD79_05395"
     CDS             835..1593
                     /gene="map"
                     /locus_tag="VFD79_05395"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002839396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HZH93518.1"
                     /translation="MIMVKTNDEILKMKKAGEILAGLHKEIKKMIKPGVTTMEIDRFV
                     EKYLEERGAYPEQKGYHGYPYATCASINDEICHGFPRNEALQNGDIVSIDMVVRFDGW
                     LADSAWSYGVGELSEEAGRLMEVTREALYLGIDKAIPGNRLGDIGHAIQKHVEENGFS
                     VVRDFVGHGIGKDMHEDPQVPHYGKPGRGARLVEGMVITIEPMVNTGEWAMKLDDNKW
                     TARTVDGSLSAQYEHTIAITADGPIILTDQGEER"
     gene            1604..>2222
                     /locus_tag="VFD79_05400"
     CDS             1604..>2222
                     /locus_tag="VFD79_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HZH93519.1"
                     /translation="MSKNYTVREEIMNGITHGLGILFGIIALIVLLLSPGAKESLAAA
                     ISFGIYGVCIIIMFSASTLYHSFRKEKVKKVLRVFDHSSIFLFIAGTYTPVVILTLEG
                     PTRVWILAGTWLVALAGIIFKIVTYGKFDKYKALSLGLYIGMGWMAIIPIKAIIGATS
                     WGFFGFILAGGLLYTVGTIFYSIKKIPYNHAIWHLFVLAASISHFI"
BASE COUNT          702 a          408 c          565 g          547 t
ORIGIN      
        1 caagagagga taccagcctg cttcttttta atattggagt aattctgact gagctgggta
       61 agttggatgg agccattaga tgctacaaaa aaataattga gataaaccca atctatgtcg
      121 atgcatacta tcagcttgga gtgataagtg aagacaaggg agagtatgag gaagctgttg
      181 aactatacaa aagtgcattg aggattgatt ctaaagacac cttgagcatg tataatctgg
      241 caaaccttta tgacttgaaa gaggaagagg gtagagccag aaacctgtat aaaaggattc
      301 ttaagcttga accggaccat ccatacacct tgaacaacct gggggccata cttgaatcaa
      361 aggaacgtta caatgaggct tatgaactta taaaaagaag cattgattca aaggatgatt
      421 ttttcatgtc acactttaac atgggtgtgg ttctaaaggc tctgggaaac cctgagaagg
      481 cactttggca ctacaaaagg tctattgaat tgaatgccaa ctaccactac tcgtacctca
      541 acatgtctgc aatatacatc gaggggggac aataccatga ggcgattgat attctgacca
      601 tgggaattgg agcaaatcca acagcccacg acctgtacta caacagagcc tgctccctgg
      661 ctatactagg tagtgaccag aaggcattgg atgacattga aagggctctt ggcatgcatc
      721 agcagctgat aaaatgggtg gaatgggata aggattttga ttccattaga ggaaaagcaa
      781 ggtttagaga aataattgga aagtacaaca tgacaaaagg aaaggaagaa gcagatgata
      841 atggtcaaaa ccaacgatga aatcttgaag atgaagaagg caggggaaat actggcaggt
      901 cttcataagg aaataaagaa aatgatcaag cctggggtta caacgatgga aattgatagg
      961 tttgttgaaa agtatctgga ggagagggga gcataccctg agcaaaaggg ataccacggt
     1021 tatccatacg ccacctgcgc ttccatcaat gacgagattt gccatgggtt tccaagaaat
     1081 gaagcccttc aaaatgggga tatagtttcc atagacatgg ttgtcagatt cgatggatgg
     1141 ttggcagatt cagcctggag ctacggggtt ggagaattgt cggaggaagc tggtagattg
     1201 atggaggtta caagggaggc cctatacctt ggaatagaca aggcaatacc aggaaatcgc
     1261 ctgggagaca ttggccacgc catccagaag catgttgaag aaaatggatt ctctgttgta
     1321 agagattttg tcggtcacgg catcggtaag gacatgcacg aagaccccca ggttccccac
     1381 tatggaaaac ccggtcgggg ggcaagactt gtggaaggaa tggtaattac catagaaccc
     1441 atggtaaaca ctggagaatg ggccatgaag ctggatgata acaagtggac tgcaagaact
     1501 gtagatggca gcctctcagc ccagtatgaa cacacaatag caatcactgc ggatggtcca
     1561 ataatactga ctgatcaggg agaggaaaga taaggtgaga taagtgtcaa aaaactatac
     1621 tgtgcgagaa gaaataatga atgggatcac ccatggactg gggatacttt ttggaataat
     1681 agccttgatt gtcctactgt tatctcctgg ggccaaggag agccttgctg cagccatctc
     1741 ctttggaatc tatggagttt gcataataat catgttctct gcctcaacct tgtaccacag
     1801 ctttagaaag gaaaaggtta agaaggtctt gagggtgttc gaccattcct caatattcct
     1861 tttcattgca ggaacctata cacccgttgt catacttaca cttgaagggc ccacaagggt
     1921 ttggatactt gctggtacat ggctggtggc cctggcggga ataattttca agatagtgac
     1981 ctatggaaag tttgacaaat ataaggcctt gtcattggga ttatacatcg gaatgggatg
     2041 gatggcaatc ataccgatta aagcgatcat tggggccacg tcctggggct tctttgggtt
     2101 tattctggca ggaggactgc tctataccgt tggcacaatc ttttacagta taaagaagat
     2161 tccatacaac catgcaatat ggcatctctt tgtattggca gcaagcatat cacattttat
     2221 tg
//
LOCUS       DASRQF010000202        11862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_869762, whole genome shotgun sequence.
ACCESSION   DASRQF010000202 DASRQF010000000
VERSION     DASRQF010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 11862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11862
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869762"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..2518
                     /locus_tag="VFD79_05405"
     CDS             113..2518
                     /locus_tag="VFD79_05405"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HZH93520.1"
                     /translation="MNKNLELLAPVGSTEALQAAVMNGADAVYLGGKLFNARHYASNF
                     DYEQLMEAVSYAHLRNVSVFVTVNILLDDEEISEAIDYVKYLYEIGVDGIIVQDIGFA
                     SLVGRIFPELQIHASTQMTINNLEGAKHLENLGFKRAVLAREVALEEIERISKNSDIE
                     LEVFIHGALCFSYSGQCLMSSLIGGRSGNRGTCAQPCRMEYSIVDKNGNLTGNLDKGY
                     YLSTRDLNTLDQIQELIGVGVKSFKIEGRMKRPEYVATVVGAYRKAIDHGSSSLTQKD
                     KTDVQQMFNREFTKGMTFGDFGRDFVSVDRPDNRGRIVGKVISSKKNSIQIQLFEDID
                     LGDGLEWDTYGKDRSGTKAQVLGKQGEKIWLERIKEPAPEGSLIRKTSSSQLLLQAQK
                     SYEHKDKEFPVDMKLSLRIGEVPELSVSYNNSLVRVTGENKVESALKAPISRERIHEQ
                     LSKLGDTVFSLGSIHMELDENAFITVKDINKLRRKAVEELGGKVISSFKRSLDSSYKN
                     LKANELTITRRNKEKARLNVRVSSQRQLDQLNLDKLDRVYIGFDEQLKENIIRLKKRG
                     LEVYYWTDKILYEKDLENLQLTVEDNIDQLDGVSTSNLGTLQFIRDRFNTNIHGDIGL
                     NSFNSHSIDYFRANGVNGITLSPELNLGQIRRIAEKTGGELEAIVYGYLPSMITRNCP
                     MAYIKGCKDDKACRTCQYADGFMLKDRMGKNFMMKRGEGFTTIYNSVPLMVLDRLEEV
                     QKSGVTSFRLDFTIEDNIIEIQEKYYDYLNRTIDKNEAVSFMNEYKSNTEVTNGHFYR
                     GVV"
     gene            2531..4912
                     /locus_tag="VFD79_05410"
     CDS             2531..4912
                     /locus_tag="VFD79_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HZH93521.1"
                     /translation="MNQKSLKVLEFFKIRDMLVELAESSPGKSIAMELMPSVDSNEVK
                     YSQNETSEALTLLMKRGNPPLFGINEVSGDVKRAELGGMLHPGSLLKISESLRVSRAL
                     KNYMRDTREESSEHYRIIGEMIQGLGVIKNLEDAISTAIIGEDEISDNASSTLRNIRR
                     QIRVKNDGIRDKLNSIITSSTHRKYLQDAIVTMREGRYVIPVKQENRGSVPGLVHDVS
                     SSGATAFVEPMAVVELNNQLRELEVKEREEIERILMELSGMVAEDAEGIRSNEVLLSR
                     LDFAFAKGKLALAMNATKPVFNEHGYLNIKKGRHPLLGKEVVPIDVYLGDSFNTLIIT
                     GPNTGGKTVTLKTVGLFTLMGQSGLHVPADFNSELAIFRKIFADIGDEQSIEQSLSTF
                     SSHMTNIVDILEEVDNTSLVLFDELGAGTDPTEGAALAMSILDHLRTMGIRTLATTHY
                     SQLKVYALTTENVTNASVEFDVETLSPTYKLLIGVPGKSNAFEISKRLGLQDYIIDRA
                     RDLLTKESIEFEDVLQAMDRDRRIAEENKSETERLKLDIQNLREQLSKEKNKTEEMRE
                     KILQKAKEEARQIVRNAKDNADLIIDELRSISSEIDKDKGRRLQEAQDLLRKEIRDRE
                     AQASTGIREVKAKKPPKNLKAGEAVEVLSLNQQGTVVTPPDDKGNVEVQIGIMKVNVS
                     VETLRRIEGKDQTSSMERSKSVKSRKTKAIKPELDIRGRNIEEGTFELDKYLDDAFIS
                     GLKEVYIIHGKGTGALREGIKSYLKGHRLVKSQRSGKYGEGGDGVTVVELKNS"
     misc_binding    4948..5133
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5198..6898
                     /gene="argS"
                     /locus_tag="VFD79_05415"
     CDS             5198..6898
                     /gene="argS"
                     /locus_tag="VFD79_05415"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005955710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HZH93522.1"
                     /translation="MINFKDKVIELLDEKISSLTAGEIEGLIEVPPSYDMGDYAFPTF
                     RLAKEYRKAPNAIAEEIASSMEGNEFFGKVQNQGPYVNFFIAREKLAEDVISEVMDKK
                     EMYGSSDMGEGKKVIVEFSSPNIAKPFHIGHIRTTVIGNSLAKIYKFLGFDTVTINHL
                     GDYGTQFGMLISAYKKWGDRQVIESNPISELLKLYVRFNQEAEDKPELQDEARYWFKE
                     LEHKNAEAMELWQWIRDISLKEFNRVYDMLNIKFDSYAGESFYSDKMPEVLEEMKEKG
                     IMKESEGALIVDLEEYGMPPALIMKKDGSTLYTTRDIAAALYRKKHYDFHKNLYVVAS
                     QQNLHFKAWIKIVELMGYEWAKDCIHIPFGMVSLEEGTLSTRKGRVVFLEDVLNKAIE
                     STRSIIDERNPNLENKDLVAKQIGIGAILFQELFNQRIKDYTFNWDRTLSFEGETGPY
                     VQYTHARTNSLLEKGGFNPEDQISFNLLTGEDEINIIRMLYDFPGIVVDAMDKNEPFY
                     ITRQIVEIAKAFNKFYNRTQIITGDEEAKKARLALVYSTKTVIKTGLGLLGIEAPNKM
                     "
     gene            complement(6934..7149)
                     /locus_tag="VFD79_05420"
     CDS             complement(6934..7149)
                     /locus_tag="VFD79_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93523.1"
                     /translation="MKHFDFDKISIQEMSKDDMLLIIEALEYTGKATNIDDFLALREN
                     ILLELSSLAELTQDQFLDYLKEETLSF"
     gene            7293..9047
                     /locus_tag="VFD79_05425"
     CDS             7293..9047
                     /locus_tag="VFD79_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HZH93524.1"
                     /translation="MSVILTTLNSKFIHSNLAIRYLKEYAKDLEDIRIEEFTINQNLG
                     EIAAKIYGLKPDLVAFSTYIWNIDQTLEVCERLKLVSPDTKILLGGPEVSFQMKDLML
                     RNAFIDYIVYGEGEETFRDLLLKKEPFGIEGLVYRHGGEVIVNPPRPLIGDIDRIPSP
                     YHSIGDEYRNKIVYYESSRGCPFNCAFCLSSTIKGVRYMSIDRVKKDLDNLIDAGVKQ
                     VKFVDRTFNANKEFSKEVMKHIIARDPGGINFHLEVTAHLIDQEQLEFFENIREGLFQ
                     FEIGVQSTHPKTIEAIGRNTDFERLSHVSKAIKSNKNIHQHLDLIAGLPHEDYKRFGE
                     SFDHIYSLRPEKIQLGFLKLLKGSELRNKQEEYGYKIIDRAPYEIMGSNWLSYDDVIS
                     LKGIEDLVEKYYNEEFFRHSLEYMLSNHFESPFKFYENFMQYWKAKGYYEYSHGREKL
                     YWILLEYYKEKSFEGPDLFLELLTLDFLENNGGKPVPNFLSQGDKLGSIDYHGILKTE
                     ELLEKFMPEYKDLPTKKLLKKVKVHYFNYNISEIVEDGYRNDGDMKGTLILFKYRDGT
                     IERCRTYDITSYVEEFSQ"
     gene            9044..9793
                     /locus_tag="VFD79_05430"
     CDS             9044..9793
                     /locus_tag="VFD79_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93525.1"
                     /translation="MKILNDIILVNKEIIQKSRSTITRNPEILLMGIVYSILSLLAGI
                     LIFNLLAGLGILLGIIYALVESAIVSSYLFVLHNVVVYNRFRWQDIKNGFTYYIRKVY
                     GVLFIFYLVNLIIGFMSNILGSAIIAILFILGLGAFVVLNPLPEALYVKDRDSLQTIL
                     YCVDFMKENWINWLVPNFILILILYLITGGVFSGGLNPFRGLSFSTSISSIVMYIIGS
                     AILSVAMVYRGHLFKLLSTSTLRKRMFMRKL"
     gene            9805..10941
                     /locus_tag="VFD79_05435"
     CDS             9805..10941
                     /locus_tag="VFD79_05435"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93526.1"
                     /translation="MSAGERYFSSKTELVSFIQLKDESQVSLKGFELDQTIPLPVVTD
                     KLLSQLNKVDSDQGITLERVIEGILYLMGADQDFLHHDYYGRILESYNPDMEKDLFKK
                     AIMALESGNYTESGLYLRAFNNLYGTKEGMFYYAMVLEAMGKDGIEEGKIEDGNSFLE
                     ESTAILEELLDEDEKYYPAYYKLGYHYKFYEQYVKAKLTWDKVLIYDPDENRRQEIRQ
                     ELDILDPEYRVELGLTYMNNMNYEKAIEVLSKLMPKHIKNWYANYLLGMAYRGYGDNQ
                     MAMEYFYGAMDSDPTIPEVYNELGISYFNENDIEKAMEIFTQGIEAGNVDDYRLYFNR
                     GLGYLNLGNIDKGYDDIYKAHQLNPGDDNVKAQLNAIESFRNKQ"
     gene            10958..>11862
                     /locus_tag="VFD79_05440"
     CDS             10958..>11862
                     /locus_tag="VFD79_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HZH93527.1"
                     /translation="MIRELKELLSVKGEKMKLAVVACQDEDVLEAVVESSRLGISQPI
                     LIGSLKETREIAEKLDIDISDFELIDEEDLAAAAEIGVRMVSQGKADFIMKGLVDTSI
                     LLKAVLNKDWGLRTDSLLSHVMVYDVETYHKLLYLTDGGMNLNPSLEEKKKIIDNSII
                     VAKAMGCENVKVAALAAKEKVNPKMPATVDAGELKKMSDDGVFGKDVTVEGPLALDLA
                     LSKEAAKIKRIESPVAGDADILLVPNIEMGNGIGKAITYLANGKSAGIIMGAKAPVVL
                     VSRADDKDTKLYSIALGNMISSYLS"
BASE COUNT         3981 a         1954 c         2832 g         3095 t
ORIGIN      
        1 taaggagtat aaagataagc actttaagac acaaatggaa attgtggaac taaagaagca
       61 acttaaggag acacttgagc ttcttgatga gaactgatca ggaggagaat ttttgaataa
      121 aaacttggaa ttattagccc ccgtagggag cacagaagcc ctccaagccg cagtgatgaa
      181 cggagcagat gctgtgtacc ttggaggaaa gttgtttaac gcgcggcatt atgcctccaa
      241 ttttgattat gagcaattaa tggaggctgt ttcttatgcc catttaagaa atgtttcagt
      301 ttttgtaact gtaaacatac tgttggatga tgaggaaatt tctgaagcaa ttgactatgt
      361 gaagtattta tatgaaatag gcgttgatgg aattattgta caggatattg gatttgcttc
      421 acttgttgga aggatttttc cagagcttca gattcatgca agcacccaga tgactatcaa
      481 taatctggag ggtgcaaagc atttagagaa tctaggcttc aaacgtgcgg ttctagccag
      541 agaggtagcc ttggaagaaa tcgaaaggat atccaagaat tcggatattg aactagaggt
      601 attcatccat ggggccttgt gcttttcata ttcgggacaa tgcctgatga gcagccttat
      661 tggagggaga agtggcaata ggggaacatg tgcacagcca tgtagaatgg agtacagcat
      721 agtagacaaa aacggaaatt tgaccgggaa cttggataag ggttattatc tgagcacccg
      781 tgatctgaat acccttgacc aaattcaaga actgataggg gttggtgtga agtcctttaa
      841 aatcgaaggt aggatgaaaa ggccagagta cgtggcaaca gtagttggcg cctacaggaa
      901 agccatagat catggaagct catcacttac ccaaaaggat aagacagatg tccagcagat
      961 gttcaacaga gagttcacaa agggcatgac ctttggtgat tttggaaggg attttgtttc
     1021 agttgatagg ccagacaaca ggggtagaat tgttggaaag gttatttcat ctaagaagaa
     1081 cagcattcag attcaattgt ttgaggatat tgaccttgga gatggactgg agtgggatac
     1141 ttatggcaag gataggtctg gaaccaaagc tcaagtactt ggaaagcagg gagagaaaat
     1201 atggctggaa agaatcaagg aacctgcccc tgaaggctct ctcataagaa agacttcaag
     1261 ctctcagctt cttctgcagg ctcagaaatc ctatgagcat aaggacaagg agtttccagt
     1321 ggacatgaaa ttaagcttac gcattggaga agtaccagag ctttcagtaa gctacaataa
     1381 tagccttgtt agggtcacag gagaaaacaa ggttgagagt gctttaaagg caccaatctc
     1441 cagggaacgg atacatgaac aactttcaaa gcttggagat actgtctttt cattgggaag
     1501 tatccacatg gaattggatg agaatgcttt cataactgtt aaggacataa ataaactaag
     1561 aagaaaagct gttgaagagc ttggtggaaa agttatttct agctttaaaa gaagcttgga
     1621 ttcaagctac aagaatttaa aggcaaatga actaactatc actagacgca acaaggaaaa
     1681 agccagactt aacgtaaggg tcagcagtca gaggcaattg gatcagctga atctggataa
     1741 gttggataga gtttatattg gctttgatga gcagcttaaa gagaatatta tcaggcttaa
     1801 aaaaagaggc ttggaggttt attattggac tgacaagata ctttatgaaa aggacctgga
     1861 aaaccttcag cttacagttg aagataatat tgatcagctg gatggagttt ccacatccaa
     1921 tctggggaca ctacagttta taagggatag atttaacacg aatatccatg gggacattgg
     1981 cctaaacagc tttaactctc attccataga ttattttaga gctaatggag taaatggtat
     2041 aaccttgtca cctgagctta atcttggaca gattagaaga atagccgaaa aaacaggcgg
     2101 ggaacttgaa gcgatagtct atggttactt gccatccatg atcacaagga actgtccaat
     2161 ggcatatata aaaggttgca aggatgataa agcctgtaga acctgtcaat atgcggatgg
     2221 atttatgcta aaggatagga tgggcaagaa tttcatgatg aagagaggag aaggatttac
     2281 aaccatatat aactctgttc cgctaatggt tctagataga cttgaagagg tccagaagtc
     2341 aggtgtaaca tccttcagac ttgattttac aattgaggat aatataattg aaattcaaga
     2401 aaaatactat gattatttga accgaacaat cgataaaaat gaagctgtca gctttatgaa
     2461 tgaatacaaa tccaatactg aggtaacaaa cggacacttt tacagaggtg tcgtttagcg
     2521 gggtgatttt ttgaatcaaa aaagcttaaa ggtgctagaa ttttttaaga ttagggacat
     2581 gcttgtggaa ctggcggaat caagtccagg caagagtatt gcaatggagc tgatgccttc
     2641 tgttgactcc aatgaagtta agtattccca aaatgaaacc agtgaagcat tgacactgct
     2701 tatgaaaagg gggaatccac ccctttttgg aataaacgag gtaagtggtg atgtcaagag
     2761 ggctgaactt ggaggaatgc tacatcctgg ttcccttctg aaaataagtg aaagccttcg
     2821 ggtttcaaga gcattgaaaa actacatgag ggacacgaga gaagaaagct ctgagcacta
     2881 cagaatcatc ggagagatga ttcaagggtt gggtgttata aaaaatctgg aggacgccat
     2941 aagcactgca ataatcggag aggatgaaat ctccgacaat gcaagctcaa ccctaaggaa
     3001 tatcagaaga caaatccgag tgaagaatga tggaataaga gacaagttaa acagtataat
     3061 cacttcatca acacatagaa agtatcttca ggatgccata gtaacaatgc gtgagggaag
     3121 gtatgtaatt ccagttaagc aggagaacag gggaagcgta ccagggcttg tgcatgatgt
     3181 ttcatcaagt ggagcaactg cctttgtgga acctatggct gttgttgagc ttaacaatca
     3241 gcttagggag cttgaagtca aggaaagaga agagattgag aggatcctta tggaactctc
     3301 tggaatggtt gcagaggatg ctgaaggaat aagaagtaat gaagtattgc tttcgaggct
     3361 ggattttgca tttgcaaagg gaaagcttgc tttggccatg aatgcaacaa agccggtttt
     3421 taatgagcac ggatacttaa atattaagaa gggaaggcat ccattgctgg gaaaggaagt
     3481 tgttccaatt gatgtctacc ttggagatag ttttaataca cttatcatta cgggtccaaa
     3541 cacaggagga aaaactgtaa ccctaaagac ggttggccta tttacactga tgggtcaaag
     3601 tgggctccat gtaccagcag attttaattc ggaactggct atctttagaa agattttcgc
     3661 agacataggt gatgagcaga gtattgagca gagtctttcc accttttcat cccatatgac
     3721 caacatagtt gacattctgg aagaggttga caataccagt ctcgtacttt ttgatgagct
     3781 tggtgcaggt actgacccaa cagagggagc tgcacttgca atgtccattc tggaccatct
     3841 caggacaatg ggtataagaa ccctggccac tactcattac agtcaattga aggtgtacgc
     3901 tctgaccact gaaaatgtca caaatgcctc agttgaattt gacgtagaga cattaagtcc
     3961 cacatacaag ctattgattg gagtaccagg taaatcaaat gcatttgaaa tttccaaacg
     4021 acttggcctc caggattata taatagaccg ggctagagac cttttgacaa aggaaagcat
     4081 tgaatttgag gatgttcttc aggccatgga cagagatcga aggatagcag aggagaacaa
     4141 gtccgagaca gagaggttaa agcttgatat acaaaaccta agggaacagt tgagtaagga
     4201 aaagaacaag acagaggaaa tgagggaaaa aatccttcaa aaggcaaaag aggaagcgag
     4261 acaaattgta agaaatgcaa aagacaacgc agatttaatt atagatgagc tgagaagcat
     4321 ttccagtgaa attgataagg acaagggcag aagacttcag gaagcacagg atttgcttag
     4381 aaaggaaatc cgggacaggg aagcccaagc tagcactgga ataagggaag taaaagctaa
     4441 aaagcctccc aagaacctaa aggcaggaga agcagtggaa gtcctttctc taaatcagca
     4501 gggtactgtt gtcacaccac ctgatgacaa gggtaatgta gaggtacaga taggaataat
     4561 gaaggtaaat gtatctgttg aaacccttcg gagaattgaa ggaaaggacc agaccagctc
     4621 catggaaagg agcaagtctg ttaagtccag aaagactaag gccataaagc ctgagctgga
     4681 cataagaggt aggaatatcg aagaaggaac cttcgaactg gataagtacc tggacgatgc
     4741 attcatatct gggcttaagg aggtatacat tatccacggc aaaggtacgg gagccttgcg
     4801 agaaggaatc aagtcctatc ttaaaggcca cagacttgtt aaatcacaga gatcaggtaa
     4861 gtatggagaa ggtggagacg gagttactgt ggttgaattg aaaaacagtt gaaatttcaa
     4921 ctgaaatcgg ttatatttat tgatatgata aaacctttga taagaagagt aggggataag
     4981 ctgtccaaag cgagctggaa ttaggtgcaa gccagcgaca acaaatccgt tgaaaagagt
     5041 cttggaggtt acttaggtac gttaatcgcg ttaagatttc gagtgggctt aggccaataa
     5101 gagtggtaac acggggtgac tctcgtctct tgatcagggg atgggagttc ctttttttgt
     5161 ataaataagt taattacaat ctagggaggg aaaaacattg ataaatttta aggataaggt
     5221 aatagaactg ctggatgaaa agataagcag cttaacagct ggagaaattg aaggcctaat
     5281 tgaggtacca ccttcatatg acatgggaga ttacgctttt ccaaccttta ggctggctaa
     5341 ggagtacagg aaggctccca atgcaatagc agaggaaata gcttccagca tggaagggaa
     5401 tgaatttttc gggaaggtcc aaaaccaggg tccctatgta aacttcttca ttgccaggga
     5461 aaagctggcg gaggatgtta tatcagaggt tatggataaa aaggaaatgt atggttcttc
     5521 tgatatggga gaaggcaaga aggtaattgt tgagttctca tcaccaaata ttgccaaacc
     5581 attccacata ggccacataa ggactaccgt aattggaaac tcattggcta aaatctataa
     5641 atttcttgga tttgacacag tgaccataaa ccatcttgga gactatggaa cacaatttgg
     5701 aatgcttata tctgcctata aaaaatgggg cgatcgtcag gtcattgaat ctaacccaat
     5761 ttctgaactc ttaaagctat atgtaagatt caaccaggaa gctgaagata agccggaact
     5821 tcaagatgag gctaggtatt ggttcaagga gcttgagcac aagaatgctg aagccatgga
     5881 actgtggcag tggataaggg atatcagtct taaggaattc aacagggttt acgatatgtt
     5941 gaacatcaaa ttcgattcat atgcaggtga gagcttctac tcagacaaga tgccagaggt
     6001 tcttgaagag atgaaggaaa aggggattat gaaggaatcc gaaggggcat tgattgtaga
     6061 ccttgaagaa tatggaatgc caccggcact gatcatgaaa aaggacggct caaccctgta
     6121 tacaacaagg gacatagctg cagccttgta cagaaagaag cactatgatt tccataagaa
     6181 tctttatgtt gtagcatcac agcagaatct ccacttcaag gcctggataa agatagttga
     6241 actgatgggg tatgagtggg ccaaggattg tattcatata ccatttggca tggtaagctt
     6301 ggaggaagga accctatcca caagaaaggg tcgggtagtt ttccttgagg acgtcttgaa
     6361 caaggcaatt gagagcacta gaagtattat cgatgaaagg aatcctaatc ttgaaaacaa
     6421 ggatcttgtg gcaaagcaga taggaatagg tgcaattcta ttccaagagc tgtttaacca
     6481 gagaataaag gactacactt tcaattggga taggactctg agttttgaag gtgaaacagg
     6541 tccttatgta cagtacactc atgccaggac taactcccta ttggaaaagg gaggctttaa
     6601 tccagaggat caaatcagct tcaatttgct gactggtgag gatgaaataa acattataag
     6661 aatgctgtat gattttccag gcattgtagt agacgccatg gataagaacg agccattcta
     6721 catcactaga cagatcgttg aaattgcaaa ggctttcaat aaattctata atagaactca
     6781 gattatcact ggggatgagg aggcgaaaaa agcaagactt gcacttgtct actcaaccaa
     6841 gacagtaata aagactggtc ttggcctatt gggaatcgaa gcaccaaaca aaatgtagtt
     6901 taacctaaaa atcaatttaa aaagagacct tatctaaaat gatagggtct cttcctttag
     6961 gtaatctagg aattggtctt gggtcagttc tgcaagggag gataattcca aaagtatgtt
     7021 ttccctaaga gctagaaaat catctatgtt tgtagccttg ccagtatact caagagcctc
     7081 tattatcagc aacatatcgt ccttagacat ttcctggata ctaatcttgt caaaatcaaa
     7141 atgcttcatc ttatgtcccc tttcatttgt tctactatac attctagtac ttttcagacc
     7201 attattcaat agaattaatc ctaattatta ttggccttgt cttgtgtttt aaggtaaaat
     7261 agtaatggtg gtttatagaa aggaagatat caatgtcagt tatactaaca acactcaatt
     7321 ctaagttcat tcactcaaac cttgctatac ggtacctgaa ggagtatgca aaggaccttg
     7381 aagatataag aattgaggaa tttacaatta atcaaaatct aggtgagatc gcggccaaaa
     7441 tctatggatt gaagccagac cttgttgctt tttcaaccta tatatggaac atagaccaaa
     7501 ccttggaagt gtgtgaaagg ctaaagcttg ttagtccaga cactaagata cttctaggtg
     7561 ggccagaggt atccttccaa atgaaggacc ttatgttaag aaatgccttt attgattata
     7621 ttgtttatgg cgaaggtgaa gaaaccttta gagacctttt attaaaaaaa gaaccatttg
     7681 gaatagaggg actagtctat aggcatgggg gagaagtaat cgtaaatcca ccaaggccac
     7741 tgattggaga tatagatagg attccatctc cctaccattc cattggggac gaatacagga
     7801 ataaaattgt ttactacgaa agctcaagag gttgcccctt caattgtgca ttctgccttt
     7861 cctcgacaat caagggtgtc aggtatatga gtattgacag ggtcaaaaaa gacttggaca
     7921 acctaattga tgcgggtgta aagcaggtta agtttgttga taggacattt aacgcaaaca
     7981 aggagttttc caaggaagta atgaaacaca ttattgcaag ggatccaggt gggattaact
     8041 ttcaccttga ggtaacagcc catcttatag atcaggaaca gttggagttt tttgaaaaca
     8101 ttagggaggg cttgttccaa tttgagatag gtgttcagtc aacccatcca aagacaatag
     8161 aagccattgg gagaaatact gattttgaaa gactgtcaca tgtaagcaag gcaatcaaga
     8221 gcaacaaaaa cattcatcag catcttgacc tgatagcagg ccttccccat gaggactaca
     8281 aaaggtttgg agaatccttt gaccacattt actcattaag gccagagaaa atacagctgg
     8341 gtttcctaaa gctcttaaag gggtcagaac ttaggaacaa gcaggaggaa tatggttaca
     8401 agataattga tagggctcct tatgaaatca tgggatccaa ttggcttagc tatgatgatg
     8461 taatatcctt gaagggtata gaagacctgg tggaaaaata ctacaatgaa gaattcttca
     8521 gacatagcct tgaatacatg ctaagcaacc attttgaaag ccccttcaaa ttttacgaga
     8581 actttatgca atactggaaa gcaaagggtt actacgagta ttcccatgga agagaaaagc
     8641 tctattggat actgcttgag tattacaaag agaaatcatt tgaaggtcct gatttgtttt
     8701 tagaactgct gaccctggac ttccttgaga ataacggtgg caaaccagtt cccaactttc
     8761 tcagccaagg agacaagcta ggttcaattg attatcatgg cattctcaag actgaggagt
     8821 tgcttgaaaa attcatgcca gaatataaag accttccaac aaaaaaacta ttaaagaaag
     8881 tgaaagtaca ttatttcaat tacaacatca gtgaaattgt agaggatggg tatagaaatg
     8941 atggggatat gaaagggaca ttgattctat tcaaatacag ggatggtaca attgaaagat
     9001 gtaggactta cgatattaca agctatgtgg aggaatttag ccaatgaaaa tattaaatga
     9061 cataatcctt gttaacaagg aaattataca aaaatcaaga agtacaatca ctagaaaccc
     9121 tgaaattctt ctaatgggaa tagtatactc catcctgagt ctgttggcag ggatacttat
     9181 tttcaatttg ctggcaggat tgggaattct actgggtata atctatgcct tggttgaaag
     9241 tgcaattgtt tcaagttatt tatttgtact gcacaatgtt gtagtctata acagattcag
     9301 gtggcaggat ataaagaacg gatttaccta ctacatcagg aaggtgtatg gtgtactgtt
     9361 catattctac ctagtaaacc taataatagg ttttatgtcc aacatactgg gatctgcaat
     9421 aattgcaatc ctatttatcc ttggactagg tgcttttgta gtattgaatc cattgccgga
     9481 ggctttgtac gtaaaagaca gagattccct tcaaactatt ctctactgtg ttgatttcat
     9541 gaaggaaaac tggataaatt ggctggtgcc aaacttcatt ctaatactaa tcctgtatct
     9601 gatcactggt ggagtgttta gcggtgggct gaatccattt agaggtttgt catttagcac
     9661 ctcaatttcg tccattgtca tgtatatcat tggaagtgca attctttcag tagctatggt
     9721 atatagggga cacctgttta agctcctgag tacatcgact ttaagaaaaa gaatgtttat
     9781 gaggaaacta taaggaggaa ttctatgtca gcaggagaaa gatattttag ttcaaagact
     9841 gagctggtaa gctttataca gcttaaggat gaaagccagg tcagtctaaa ggggtttgaa
     9901 ttggaccaaa caatccccct tccagtagtt acagacaagc tcctcagcca gttgaacaag
     9961 gttgacagcg accagggaat taccttggag agggtgatag aaggaattct ttacctgatg
    10021 ggagctgacc aggattttct acaccatgat tattatggtc gaatacttga atcctacaac
    10081 ccagatatgg agaaagacct gtttaaaaag gccataatgg cacttgaaag tggaaattat
    10141 actgaaagtg gtctttatct cagggcattc aataaccttt acggtacaaa ggaaggcatg
    10201 ttctactatg ctatggtcct tgaagccatg ggaaaggatg gaattgaaga aggaaaaata
    10261 gaggatggca acagcttcct tgaggaatca acagctatac ttgaagaatt gttggatgaa
    10321 gatgaaaagt actatccggc atactataag cttggctacc actataaatt ctatgagcaa
    10381 tatgttaagg ccaagctgac gtgggacaag gttcttatat atgacccaga tgaaaacaga
    10441 agacaggaaa taagacagga actggatatc ctggatccag agtatagggt tgagcttggg
    10501 ctgacatata tgaacaacat gaattacgaa aaagctatag aggttttatc aaagcttatg
    10561 ccaaaacata ttaagaactg gtatgcaaat tatttgctgg gcatggccta caggggctat
    10621 ggagacaacc aaatggccat ggaatacttc tacggggcaa tggacagtga ccctaccata
    10681 ccagaggtat acaatgaact gggaatttca tatttcaatg aaaatgatat tgaaaaggca
    10741 atggagattt tcactcaagg aattgaagct gggaatgttg atgattatag attgtatttt
    10801 aacagggggc ttggatatct aaacctgggt aacattgata agggatatga tgacatatat
    10861 aaggcccatc agttaaatcc aggggatgac aacgtaaagg ctcagcttaa tgcaatagag
    10921 agcttcagaa acaaacaata agataggggg actagacatg ataagggaac taaaggaact
    10981 gttgtcagtt aagggtgaaa aaatgaagct tgctgtggtg gcctgccagg atgaggatgt
    11041 ccttgaagct gttgttgaga gttcaaggct tggtataagc cagccaatat taataggatc
    11101 attgaaagag acaagggaga tagcggaaaa gttggacatt gatatttcag actttgaatt
    11161 aatcgatgag gaagatctgg cggcagcagc tgaaatcgga gttcgaatgg ttagccaggg
    11221 caaggctgat tttataatga agggacttgt ggatacatcc attcttctaa aggcagtgct
    11281 aaacaaggac tggggactta gaacagacag ccttcttagc catgtaatgg tatatgatgt
    11341 tgaaacctat cacaagttgc tttacctgac tgacggtgga atgaacctga atccctcact
    11401 ggaggagaag aagaagatta tagataatag tatcattgtt gctaaggcaa tgggatgcga
    11461 aaatgtcaag gtagctgctt tggctgccaa ggagaaggta aaccctaaaa tgcctgcaac
    11521 agtcgatgct ggtgaattga aaaagatgtc agatgatggc gtatttggga aggatgttac
    11581 tgttgagggt ccattggcat tggatcttgc cctgtcaaag gaagccgcca aaatcaagag
    11641 gatagaaagt ccagttgcag gtgatgctga tattttgttg gttcccaaca tagaaatggg
    11701 taatggaatc ggcaaggcca taacatacct tgcaaatgga aagtctgcag ggatcataat
    11761 gggggcaaag gcaccggtgg ttttggtatc aagagccgat gacaaggaca ccaaactata
    11821 ctcaattgca ctggggaata tgatttccag ttatttgagt aa
//
LOCUS       DASRQF010000203         2088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_884546, whole genome shotgun sequence.
ACCESSION   DASRQF010000203 DASRQF010000000
VERSION     DASRQF010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884546"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..>2088)
                     /gene="ftsH"
                     /locus_tag="VFD79_05445"
     CDS             complement(273..>2088)
                     /gene="ftsH"
                     /locus_tag="VFD79_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005957545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HZH93528.1"
                     /translation="KPIMYYYLIALIVIIVLNMVVFPRMMQSDIEQIDYGTFLQMLED
                     GEIDVVEIKDNDIFIQEKGSDAVFVTGTMDDPELVRKLTDSDVKFSREISDTTPSLMS
                     TIFSWIFTIVIFVAIGQLFARSMQKRMGGGNDVLSFGKSNAKIYVESKTGKTFEDVAG
                     QDEAKEALEEIVDYLHNPGKYKEIGANLPKGALLVGPPGTGKTLLARAVAGEAKVPFF
                     SISGSEFVQMFVGMGAAKVRDLFKQAQEKAPCIVFIDEIDTIGKKRDGGGIGGNDERE
                     QTLNQLLTEMDGFDGKEGVVILAATNRPESLDKALLRPGRFDRRVPVELPDLKGRENI
                     LKVHAKAVKVDPDLDYNAIARATAGASGADLANMINEAALRAVRQGRDKVSQPDLEES
                     VEVVIAGYQRKNSVISTKEKRIIAYHEIGHALVAAKQTDSDPVHKITIIPRTSGALGY
                     TMQISEQESVLMSKEELFNKIVTITGGRAAEELVFGSITTGASNDIEQVTKIARAMVT
                     RYGMSSEFDMMALETQTNMYLGGDSSLACSAETSSRIDAEVLRIIKDAHDKARKILEE
                     SMDKLRELSNYLLEMETITGEQFMEILNKKEEQEELGE"
BASE COUNT          486 a          564 c          432 g          606 t
ORIGIN      
        1 aattttcgaa acgagtgtga taactctctg atcttgaatc cgagagcgga tcatctgact
       61 ttccgaagga tacaagagag atccctacac gttgggtcgg gatgaccgcc caattcacag
      121 atcgcagttc tcaattcaca atttttaaca aaggtcgagc tttggggtct aagatacttc
      181 acattcgttc agtatgacaa cataataaag attcaacgat catgctagga acacaaaaaa
      241 caaaatgccg gcagaaatct gccggcatca tcttattcac ctaattcttc ttgctcttcc
      301 ttcttgttca gaatctccat aaactgctca ccggtaattg tctccatctc aagaagataa
      361 tttgaaagct ctctcagctt gtccatactc tcctcaagaa tcttcctggc cttgtcgtgg
      421 gcatccttga tgatcctcag tacctcagca tcaatcctgg atgaggtctc tgccgagcat
      481 gcaagggatg aatcccctcc aaggtacatg ttggtctggg tttcaagagc catcatatca
      541 aactcgctgc tcattccata ccttgttacc attgcccttg caatctttgt gacctgctcg
      601 atgtcgttgg atgcacctgt tgttatggac ccgaatacaa gctcctctgc cgcacgtcca
      661 ccagtgatgg taacaatctt gttaaaaagc tcctccttgc tcataagcac actttcctgc
      721 tctgatatct gcattgtgta gcccagggct ccggaggttc ttgggataat tgttatcttg
      781 tggactgggt ctgagtctgt ttgcttggca gcaaccaggg catgcccgat ttcgtggtag
      841 gctatgatcc tcttctcctt ggtggagatt acggaattct tcctttggta tccggcaatt
      901 acaacctcaa ctgattcttc aaggtcaggc tgactcacct tatcccttcc ctgccttaca
      961 gcacggaggg cagcctcgtt tatcatattg gcaaggtcag ctccggaagc tcctgcggtt
     1021 gcccttgcga ttgcattata gtcaaggtca ggatcaacct tgacagcctt ggcgtgaacc
     1081 ttcaggatgt tctcccgtcc ctttaggtca ggtagttcaa ccggaaccct cctgtcgaac
     1141 cttcccggcc ttagaagggc cttgtcaagg gactcaggcc tgtttgtggc agcaaggata
     1201 accacaccct ccttaccgtc aaaaccatcc atctccgtca ggagctggtt aagtgtctgc
     1261 tccctttcgt cgttacctcc gattcctcca ccgtcacgct tcttgccgat ggtgtcaatc
     1321 tcatctataa agactatgca gggtgccttt tcctgtgcct gcttgaaaag gtcccttacc
     1381 tttgcagctc ccattccaac aaacatctga acgaattcag aacctgaaat tgagaagaag
     1441 gggacctttg cctctcctgc aaccgccctt gcaagaaggg tcttgcctgt accagggggt
     1501 ccaaccaata gggccccctt gggtaggttt gcaccgattt ccttgtactt gccggggttg
     1561 tgaaggtagt caactatctc ttcaagggcc tccttggcct cgtcctgtcc tgccacatcc
     1621 tcaaaggtct tgcctgtctt tgattcaaca tagatttttg cattggactt tccaaaggac
     1681 agtacatcat ttcctccgcc catcctcttc tgcatggacc tggcgaagag ctggcctatg
     1741 gcaacaaaga tcactattgt aaatatccat gaaaatattg tactcataag ggatggtgtg
     1801 gtgtctgaaa tctcccttga gaatttcaca tccgaatctg tcaacttccg gaccagttcc
     1861 gggtcatcca ttgtccctgt tacaaatact gcatccgatc ccttttcctg aataaatatg
     1921 tcattgtcct ttatttccac aacatcaatt tccccgtcct ccagcatctg aaggaaggtt
     1981 ccgtagtcta tctgctctat atctgactgc atcatccttg gaaacacaac catgttcagg
     2041 actattatca ctattaaggc gataaggtag tagtacatga tgggcttc
//
LOCUS       DASRQF010000204         2359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1145591, whole genome shotgun sequence.
ACCESSION   DASRQF010000204 DASRQF010000000
VERSION     DASRQF010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145591"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="VFD79_05450"
     CDS             complement(<1..973)
                     /locus_tag="VFD79_05450"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HZH93529.1"
                     /translation="MAKQKKKKKPVDFLIFISVVTLVFIGIIMVYSASWPEGMVRFED
                     GSHYARRHIQWAVVGLLGMVLTMNIDYRFWKRISLPIYITAIVLGLLIFTPLGMELKG
                     ARRWIDLGFTTFMPSDAIKLGSIVFFAAFLDNKKERIRRIGQGAIPAFILIGVSAGLV
                     YLQRDLSTTATVAGTLFAMFFVAGMWLPVLGVIIAGVGIFARYAIFSEGNEYRQRRVF
                     SFMDPFADKLGDGWQIVQSLYAMGSGGFLGVGLGQSRQKFFYIPEAYNDFIFSIIGEE
                     LGLVGSMFVLLLYAMIIWRGYTVALHAKDAFGSYLATGITSLLAIQSF"
     gene            complement(978..2336)
                     /gene="murD"
                     /locus_tag="VFD79_05455"
     CDS             complement(978..2336)
                     /gene="murD"
                     /locus_tag="VFD79_05455"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HZH93530.1"
                     /translation="MLIKGKKILVMGIGITGISAMKMLMDYNNELMVYDEKPRDEIAE
                     ILDSNGLDQVGIIQEDSKFLIQDVDFVVKSPGIPPDHHMVEFAGEKDIPVISDIELAF
                     NIGIHGTVVGITGTNGKTTSTMLAGEILKKAGIKTHLAGNMGTGILQVVDHLQDEDYL
                     VLELSSFQLEHTYLFRPDVALITNITEDHLDWHGSIENYMASKFKIFANQSADNYLVL
                     NWDDINLRKLEKKLKPKVIWFSTVEDLEEGVFLRDGSIVYRMDGVEETVLSLDHIRIP
                     GSHNVENICGVVGIMKALGIDSETIRDTVENFKGVEHRIEFVLEKDGIAYYNDSKGTN
                     VQSSLKAVQAIGRPIVLIAGGYDKGADYSDLMDELANKSRALILMGETAERISKAAQE
                     KGISNIYIVDSMEEAVSKAVSAGMSGDAVLLSPACASWDMYKNFEERGKHFKRILVEM
                     TE"
BASE COUNT          613 a          627 c          433 g          686 t
ORIGIN      
        1 taaatgactg aatcgccaat agggatgtga ttcctgtggc aagatagctg ccaaaggcat
       61 cctttgcatg gagggcgact gtatatcctc tccatattat catagcatag agcaggagca
      121 caaacataga ccccacgagt ccaagctcct ctccaattat cgaaaagata aaatcattgt
      181 aggcctcggg tatatagaag aatttctgcc tgctctgtcc cagacctact ccaagaaatc
      241 ctcccgatcc cattgcataa agggattgta caatctgcca gccgtctccc agcttgtctg
      301 caaatgggtc cataaatgaa aacaccctcc gctgcctgta ttcgttaccc tcggaaaaaa
      361 ttgcatacct ggcaaatatt cccacacctg caattatgac tcccaatacg ggtagccaca
      421 ttccagctac aaaaaacatg gcaaacaatg ttccagcaac tgttgcagtt gtacttaggt
      481 ccctctgaag gtaaacaaga cccgctgata ctccaataag aatgaaggct ggtatggctc
      541 cctggccaat ccttcttatc ctctccttct tgttgtccag gaatgccgcg aagaagacga
      601 tgcttccaag cttaattgca tccgatggca taaaggttgt aaagcctagg tcaatccatc
      661 tccttgcccc cttcagttcc atgcccaaag gagtgaatat caacagtccc aggacaatgg
      721 cggttatgta gattggaagg gagattcttt tccagaacct gtagtcaata ttcatggtaa
      781 ggaccattcc aaggaggccc accacagccc attgtatgtg ccgtcttgca taatggctgc
      841 catcctcgaa ccttaccatt ccttcaggcc aactggcgct gtaaaccatt attatgccta
      901 tgaacacaag ggtcacaacg gaaataaaaa ttaaaaaatc tacaggcttc ttcttcttct
      961 tctgctttgc catggtatca ctccgtcatt tccacaagga tcctcttaaa atgcttgcct
     1021 ctttcttcaa aattcttgta catgtcccag ctggcgcatg caggcgaaag gaggaccgca
     1081 tctccagaca tacccgctga aacagccttg ctgaccgctt cttccatgga gtcaacaata
     1141 tatatattgg aaataccctt ttcttgggct gctttactga ttctttcagc tgtttcaccc
     1201 attaggatca aggccctgga cttgttggct aactcatcca taaggtcgct gtagtcggca
     1261 cccttgtcgt aacccccagc aattagtact atgggcctcc cgattgcctg tactgccttt
     1321 agtgaagact ggacatttgt tcccttggag tcgttatagt aggctatccc gtccttctcc
     1381 aaaacaaact caatcctatg ctccactcct ttgaaatttt caactgtatc cctaatagtt
     1441 tcactgtcaa ttcctagggc tttcattatc cccaccacgc cacatatgtt ctcaacattg
     1501 tggcttccag ggattcttat gtggtccaga ctcaatacag tttcctcaac tccatccatc
     1561 ctatatacaa tagacccatc tcttaagaat actccctctt ccaggtcttc aaccgtgcta
     1621 aaccagatga ctttaggctt aagcttcttt tccagctttc ttagattaat atcatcccaa
     1681 ttgagtacca ggtagttatc agcactttga tttgcaaata tcttgaactt tgaagccatg
     1741 tagttttcaa tcgatccatg ccaatccagg tggtcttcgg ttatgtttgt tatcaaggcc
     1801 acatccggtc tgaaaaggta ggtatgctcc agctggaagc tgcttagctc aagtacaagg
     1861 taatcctcat cctgcaggtg atcgacaacc tgcaggatcc cagttcccat gttacctgca
     1921 aggtgtgtct tgattcccgc cttctttagg atctctcctg cgagcatggt tgaggtggtc
     1981 ttaccgtttg tccctgtgat tcctaccact gtcccatgga taccaatgtt aaaggccagt
     2041 tctatgtctg agatgactgg aatatccttc tcccctgcaa attcaaccat gtgatgatct
     2101 ggaggtattc cagggctttt cacaacaaaa tcaacatcct gaattaggaa tttgctatcc
     2161 tcctgtatta tccccacctg gtccaaaccg ttggaatcta gtatctcagc tatttcatcc
     2221 ctgggtttct catcatatac cataagctcg ttattgtaat ccattaacat tttcatggcg
     2281 gagattcctg taatccctat ccccatgaca agtatcttct tccctttgat taacatatgg
     2341 aacacttcct aacttaaaa
//
LOCUS       DASRQF010000205         3149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_132816, whole genome shotgun sequence.
ACCESSION   DASRQF010000205 DASRQF010000000
VERSION     DASRQF010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132816"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     misc_binding    complement(161..328)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(373..1173)
                     /gene="murI"
                     /locus_tag="VFD79_05460"
     CDS             complement(373..1173)
                     /gene="murI"
                     /locus_tag="VFD79_05460"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HZH93531.1"
                     /translation="MEKPIGVLDSGVGGLTVVKSIQKILPHEDIIYFGDSKNVPYGNR
                     DEDEIFRLTMAMLKYFEKRNVKMVAIACNTISTIADRFRDRFDFPIVDIITPTVDHIE
                     NMGIDKMVILGTDFTIRTKAYERLLRERRKDYEISSEKSPRLAELVDKGDYKSREIYD
                     TIGGHLESISQMGNFYNIVLACTHYPIVEDIFLELDPTLNYINPGFQQAKAMRMVLHE
                     QGTLKKEGKGSLQVLTSGSPEIYRKVLNKLEMRDVGEIRTLEIPPYKD"
     gene            complement(1190..2209)
                     /locus_tag="VFD79_05465"
     CDS             complement(1190..2209)
                     /locus_tag="VFD79_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HZH93532.1"
                     /translation="MAKRATGIQDYIVKTLNGMALGLFSSLLIGLIIRQIGVYGDIPS
                     LVYFGNVAQRMMGPAIGVGVAYSVGAPPLGIFASAVAGAIGAGSVSFDGATAAIAIGE
                     PVGAFVAALAGAELSKLVAGRTKFDIILVPMITILVGGLVGSYISPYIAAFMNGIGAI
                     INQATEMHRIPMGIIVSVIMGITLTLPISSAALAISLNLSGLAAGAATVGCAANMVGF
                     AIASYRENGMGGLVAQGLGTSMLQIPNIVRNPLIWIPSIIASAILGPVSTYLLEMRSN
                     SLGAGMGTSGLVGQFAMVETMGPSLYVFLMILMMHFVFPGLISFSVSEWMRKKGYIKR
                     GDMSL"
     gene            complement(2229..>3149)
                     /locus_tag="VFD79_05470"
     CDS             complement(2229..>3149)
                     /locus_tag="VFD79_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009344977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZH93533.1"
                     /translation="EKDLRIRQQEVYRKLPRVKQLDDEIRATGLRIAKRVLLKPEDSE
                     SAAKEARKKIESLKMEKAFVLTEGNISPDYLEPRYTCNLCKDTGYVEDSKRCNCMKQL
                     LINRSYKMSNLENILHKENFQKFNIDLFSQEAFDGEDLTPRENMLDILSYAEGFIGNF
                     EENNGENLLFYGTTGLGKTFLCSCIAKALLDKNKIVIYQTAFTILDILEKRRFGKGSR
                     EISDYEYDLLFQADLLIIDDLGTEISNTFTNAEIFNIVNTRLLAGKKTIISTNLTPNE
                     ISEIYTDRVFSRIFDKFIPLRFYGKDLRWE"
BASE COUNT          767 a          800 c          616 g          966 t
ORIGIN      
        1 catccagctg aagaagcctg tcgatgggat aatccccatg tctttttgcc agggctgcct
       61 ttaccccttc tgcatccatt cttattctct tgatatccaa cataataatc ctcctcctaa
      121 tcgtacttct ggtaataaaa aaactcccat cccaataaaa tatagggacg agagtatacc
      181 cgcgttgcca ccctagttaa ccgcatcagc ggttcactcg aatttcctag gctcagggtc
      241 ggattctgca attccatctg acggttttca ccagccaccg actctcttaa agactttcct
      301 gcatactatt tcccttcact gccctattta attaaaatcc attatagcat atctgagttc
      361 aatgtcaata cactaatctt tgtatggagg gatttccagg gtccttatct ctccaacatc
      421 tctcatttct agcttgttca ggacctttcg ataaatttca ggcgatcctg atgtgagcac
      481 ctgaagactt cccttgcctt ccttcttaag ggttccttgc tcatggagaa ccatcctcat
      541 ggccttggcc tgttgaaaac cagggttaat gtagttaagg gtggggtcca gctcaaggaa
      601 aatatcctcg acaattggat agtgggtaca tgcaagaact atgttgtaga aattccccat
      661 ctgggatata ctctcaagat gcccgcctat ggtgtcatat atctctcttg atttgtaatc
      721 tcccttgtcc accagctcag caagtctggg actcttttca gaggatattt cataatcctt
      781 tcttctttcc ctcaggagcc tttcataagc ctttgtcctt atggtgaaat cagttccaag
      841 tattaccatc ttatctattc ccatgttctc aatatggtca acggttgggg taattatatc
      901 aacaattggg aaatcaaacc tgtccctgaa tctgtcagct attgttgaaa tggtgttgca
      961 tgctatggcc accatcttca catttctctt ctcaaagtac ttgagcatag ccattgtaag
     1021 cctaaaaatc tcatcctcgt cacgatttcc gtagggtaca ttcttgctgt ctccaaagta
     1081 aattatgtcc tcgtgtggaa ggattttttg gattgatttt acaactgtca gtcctcctac
     1141 tcctgagtcc agtactccaa taggtttctc catctaattc ctcctaaaat tatagtgaca
     1201 tgtcccctct ttttatgtag cccttttttc tcatccattc agatacggaa aatgagatca
     1261 ggccggggaa aacaaagtgc atcattaaaa tcatcaagaa tacatatagg cttggaccca
     1321 tggtttccac catggcaaac tggcctacaa ggccgcttgt ccccattccg gctccaagtg
     1381 aattgcttct catctcaagg agataggttg acacaggtcc aaggatggcc gaagcgataa
     1441 ttgagggtat ccatataaga gggttcctta ctatatttgg aatctgtagc atggaggttc
     1501 caagtccctg agccaccagt ccacccatcc cattctccct gtaggaggcg attgcaaatc
     1561 caaccatgtt ggctgcacac cctacggttg ccgctcccgc tgcaaggcct gaaaggttaa
     1621 gtgatatggc aagggctgca gagcttattg gtagggtaag ggttatgccc atgattacag
     1681 acactattat ccccatgggt attctgtgca tttcagtagc ctgattgata attgcaccaa
     1741 tcccattcat gaatgccgct atgtagggtg atatgtagct tccaaccaag cctcccacaa
     1801 ggatcgttat cattggaacc agtattatat caaacttggt ccttcctgcc accagcttgg
     1861 acagctctgc tcccgccagg gcagccacaa aggccccaac aggctcaccg attgcaatgg
     1921 ctgctgttgc tccatcaaag gatacacttc ccgcacctat ggcgccggcc accgctgaag
     1981 caaatattcc aaggggaggg gctcccacgc tgtatgccac tcccactcca attgccggcc
     2041 ccatcatcct ctgggcaaca ttgccaaaat acacaaggga tggaatatcc ccatatactc
     2101 caatctgtct gatgataagt ccgatcagca gggatgaaaa cagccctagg gccatcccgt
     2161 taagtgtctt tacaatgtag tcctgtatac ctgttgctct ttttgccatt attcgactcc
     2221 ttttaaattt attcccatct caggtctttt ccgtagaacc tcaagggtat gaatttatca
     2281 aatatccttg aaaataccct gtcggtatat atctcggata tttcatttgg tgtaaggttt
     2341 gttgatatta tggtcttttt gcctgcaagt agtcttgtgt taactatatt gaatatctca
     2401 gcatttgtga aggtgttgga aatctctgtt ccaaggtcat caattatcag aaggtccgcc
     2461 tgaaagagca ggtcatactc gtagtcagaa atctccctgc ttcccttgcc aaaccttctc
     2521 ttttcaagga tatccaggat tgtgaaggca gtctggtaga ttactatctt gttcttgtcc
     2581 agtagggcct ttgcaatgca actgcacagg aaggtcttac ccaggcctgt tgttccgtag
     2641 aaaagcaggt tttcaccatt attctcttca aagtttccta taaatccttc agcatagctt
     2701 aatatatcca gcatgttctc ccttggggtg aggtcttcac catcaaaagc ttcctggctg
     2761 aagaggtcta tgttgaattt ctggaaattc tccttgtgga ggatgttctc caggttggac
     2821 atcttgtagg acctgttgat aagaagctgc ttcatgcagt tgcacctttt tgaatcctct
     2881 acataaccag tatccttgca gagattgcag gtatacctgg gctccagata gtccggggaa
     2941 atgtttccct ccgtcaggac aaaggctttt tccatcttca gggattcaat cttcttcctg
     3001 gcctccttgg ctgcactttc tgagtcttcc ggctttaaaa gtaccctctt ggcaatcctt
     3061 agtcccgtag cccttatttc atcatccagc tgcttcactc ttggcagctt tctgtagacc
     3121 tcctgctgtc gtattctcaa atccttctc
//
LOCUS       DASRQF010000206         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_798166, whole genome shotgun sequence.
ACCESSION   DASRQF010000206 DASRQF010000000
VERSION     DASRQF010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798166"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VFD79_05475"
     CDS             <1..414
                     /locus_tag="VFD79_05475"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HZH93534.1"
                     /translation="GTVEAIHILNTTLKTPDNKVIYIPNGQLSNTSIVNYSVKDTRRV
                     DWTFEVGYEQDGDKVKSVLADIVTAHPLVLKDPEAFIRMSNHGDSAVDFTVRAWVNAG
                     DYWTVYFDIIETVKKRFDAEEISIPYPQMDIHMDK"
     gene            544..1521
                     /locus_tag="VFD79_05480"
     CDS             544..1521
                     /locus_tag="VFD79_05480"
                     /inference="COORDINATES: protein motif:HMM:NF019450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HZH93535.1"
                     /translation="MFRFKRIASLGLSLAMVLTGGSLAFANEAEEAEVVPISAPIEDQ
                     EFEYIRFEGVVEEIEETQDSHRILVKKDTDEGLDALYAYIHEDLLLLSEKNMERATIE
                     DIEEGMEVSVIYHKSTVMALSYPPLLGPDVIVLHDGAEHSTYVEYFDENLLSADGSLV
                     LNIGDETIIVDHEGNVLTKEDIYERDLVVFHSIVMTSYPGQTTPHKVIVMPEREVETP
                     QASIVLTEEVITTDDGVRMIPLRMVAEALGFEVTWNGEEKSVEVLRGTNWSTLTIGRN
                     VYNFARMLIKLETAPVIVDGTTYVPLSFADQVLQANIEIYENGSVIISE"
     gene            1768..>2407
                     /locus_tag="VFD79_05485"
     CDS             1768..>2407
                     /locus_tag="VFD79_05485"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZH93536.1"
                     /translation="MKKNGFFLSLCILLTYILSIPVSASELDLDLTPEESRFIQEHPE
                     ITMGVDPLFVPFEFIDEDGSYKGITADYLNLISERTGLVFTVRENLTWPQAYDLALSG
                     EVDMLPAISKTPEREEHFLFSHQYYEFKRVLVTRESDTDITSMDDLEGLTVAVQRNSS
                     HHSYLLSQPEINLSLYDSVENALTAVANGTERAFVGNIATTNYLIRSTGLTNL"
BASE COUNT          749 a          474 c          565 g          619 t
ORIGIN      
        1 ggtacggttg aagcaatcca tatccttaac acaacactga agactcctga caacaaggta
       61 atctacattc caaacggaca gctttcaaac accagtatag ttaactactc ggttaaggat
      121 acaagaaggg ttgactggac ctttgaggtt ggttatgagc aggacggaga caaggtaaag
      181 agtgtacttg ctgatattgt aactgcacat ccacttgttc tgaaggaccc tgaagccttt
      241 ataaggatga gtaaccacgg agacagtgcg gttgacttca ctgtaagggc atgggtaaat
      301 gcaggagatt actggaccgt atactttgat atcatcgaaa ccgtcaaaaa gagattcgat
      361 gcagaggaaa tatcaatacc atatccacag atggatatcc atatggacaa gtaacaatta
      421 gggcagggaa acctgctctt ttttgttaac ctttttttaa tccctttgtc actcttctgt
      481 taccattaag gggaatcccc ttgctataat ggacttgtca gaataattga ggagggaaag
      541 gaaatgttta gatttaaaag aatagcatca cttggactta gccttgcaat ggtccttaca
      601 ggaggctcat tggcctttgc aaatgaagcg gaggaggcag aggtggttcc catatcagca
      661 ccaattgaag accaggagtt tgagtatata aggtttgagg gagttgtcga ggagattgag
      721 gaaacccagg attcccacag gatccttgtc aagaaggata ctgatgaagg tcttgatgcc
      781 ctctatgcat atatacacga ggacctgctt ctcttaagtg agaagaacat ggaaagggca
      841 acaatcgaag acatagaaga gggtatggag gtttcggtga tctaccacaa gtcaaccgtt
      901 atggcactta gctatccgcc tctacttggg ccggatgtga ttgtattgca tgatggggct
      961 gaacactcaa catatgttga gtactttgac gaaaaccttc tgagcgcaga cggttcactg
     1021 gtcctgaata taggggatga aacaataatt gtagatcatg aaggtaatgt ccttacaaag
     1081 gaggacatat atgaaaggga tcttgttgta ttccattcaa tagttatgac atcatatcct
     1141 ggacagacaa caccccacaa ggtaatagtc atgcctgaaa gggaagtgga aactccacaa
     1201 gcatccattg tccttacaga agaggtcata accacagatg atggggtaag gatgattccc
     1261 ctaagaatgg ttgcagaggc ccttggcttt gaagtgacct ggaatgggga ggaaaagtcc
     1321 gttgaggtcc taaggggaac aaactggtca acacttacaa taggaagaaa tgtttacaat
     1381 tttgccagga tgcttataaa gcttgagacc gcacctgtta tagttgatgg aaccacctat
     1441 gttccactgt cctttgcaga ccaggttctc caggccaata ttgaaatata tgaaaacggt
     1501 agcgtgataa tttctgaata gtttctcctt ggcgagcctt cttgagcatg aattgtgcat
     1561 gagaaggctc gtttatttgt gtagaaataa aattaggatt aaaatatgcc tataatcaac
     1621 ataaatgttg ttaaatgggc ataattgggg taaaatgtag taaaatagta taataaagca
     1681 agaaagttta cataatgatt aaaagttcta aggggaagtt gaacctagag gagagcagtg
     1741 tttgggatat aataaaggag aaattacatg aaaaagaatg gattctttct tagcttatgc
     1801 atactgctta cctacatact atccataccc gtatcagcat ctgagctgga cctggacctg
     1861 accccggagg agagccggtt tatccaggaa caccctgaga taacaatggg tgtggaccca
     1921 ctctttgtcc cctttgagtt tattgatgaa gacggcagct acaagggaat aactgctgat
     1981 taccttaacc tgataagtga aaggacagga cttgtcttta ctgtcaggga aaatctgacc
     2041 tggccacagg cctatgattt ggccctttcc ggtgaggtgg acatgctccc agcaatctca
     2101 aaaacccctg aaagggaaga acacttcctc ttttcccacc aatactatga gttcaagagg
     2161 gttcttgtca caagagagag tgatacggac atcaccagca tggacgacct tgagggtcta
     2221 accgttgcag tccagagaaa cagctcccac cacagctacc ttttatccca gcctgagatc
     2281 aacctaagcc tgtatgactc tgtagagaat gctttgacag cagtagccaa cggcactgaa
     2341 agggcatttg ttggaaacat tgcaaccaca aattacctta tacggtccac tggcctaacc
     2401 aacctaa
//
LOCUS       DASRQF010000207         3741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1331536, whole genome shotgun sequence.
ACCESSION   DASRQF010000207 DASRQF010000000
VERSION     DASRQF010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331536"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VFD79_05490"
     CDS             complement(<1..417)
                     /locus_tag="VFD79_05490"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HZH93537.1"
                     /translation="MMSFKTIKKQTQDLLTFGLFARLLNFLKGILLAFFIGANFRTDT
                     YLVAFSASVFLIGIVADALIVSLIPIYHQIDRRDGKKGRFEFTHNLISYWTILGLILM
                     VLNFLLAPFIVKLFGPGFGPEGFNQAVRLFRYGAPIV"
     gene            complement(463..1260)
                     /gene="proB"
                     /locus_tag="VFD79_05495"
     CDS             complement(463..1260)
                     /gene="proB"
                     /locus_tag="VFD79_05495"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HZH93538.1"
                     /translation="MDDMMIRNANKIVIKVGSSTITSQDGTIDKDFLDNFILQVKTLM
                     DQGKKVIIVSSGARIAGVSTIGKWSRQQDINYKQALCSIGQVELMDSYRRIFNKHGIH
                     IGQILLTKDDFEHKERTLNIRNTLFTLIDEGVVPIVNENDTVSVDEIKIGDNDMLGAY
                     TTVVWGGDLLLILSDIDGIYDCNPNDNPQACLVDVVEDIDKLEKEISIGETSSFGTGG
                     IKTKLKAARMVNSYGIPMIVAHGKTKDIITQISAGEKKGTLFLPRKA"
     gene            complement(1268..2509)
                     /locus_tag="VFD79_05500"
     CDS             complement(1268..2509)
                     /locus_tag="VFD79_05500"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HZH93539.1"
                     /translation="MISLQEKGALLVESERKLAVSSTHEKNLALREVAHSIDHNREEI
                     LKANALDVENARKSGMKEGLVDRLLLDHGRIDGIIESLYTVIKLPDPIWKSDRVWTLE
                     NGLTISKMTVPLGVIGIVYESRPNVTVDAFGLALKSGNAILLRGSSTAIHSNVALVKA
                     IKEGLKKTDIPEGVVEFIDDTDRDLVLQMLRSNDFLDLVIPRGSGALIDFVVKNATVP
                     TLQTGEGNCHVYIDDTANLHDGVKIVVNAKTQRVGVCNACETLLVNRKVAKDFLPMAY
                     EALKDKVELRADEETREIISAKAATEEDWGTEFLDFILAIKVVEDVDEAIAHINKYGT
                     KHSESIITETLGNALKFQRQVDASTVYVNASTRFTDGGEFGFGAEMGISTQKTHARGP
                     VGLEQLVTHKYLVMGQGQTRK"
     gene            complement(2588..3160)
                     /locus_tag="VFD79_05505"
     CDS             complement(2588..3160)
                     /locus_tag="VFD79_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HZH93540.1"
                     /translation="MKNIMVAGVGGQGLVLATKIIAEAALKAGLDVKTNDVVGLSQRG
                     GMVWGSARIGDVVHSPNIPAGEGDILLGMEPLEALRWKGLLKKGAVVVINEKRTYPSS
                     VLLEQRKYPEKEIEDLEKDYVVIRVDAVKEAKDAGNIKAANTVVLGILARHMDIEKEI
                     WEEVLLENVPPKAKDANIKAFNIGYEYGED"
     gene            complement(3175..>3741)
                     /locus_tag="VFD79_05510"
     CDS             complement(3175..>3741)
                     /locus_tag="VFD79_05510"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZH93541.1"
                     /translation="RNGDEAPLVSVIGDGTFFHSGMTGFVNLLHQMDEDDNMTFIILE
                     NSTTAMTGGQHNASSGNFNQADDMKVDIRAMLGAIGFTNVREVDQFDYKAMKEAVKEE
                     TGRKGTSVIITTRPCALRFKIKEKNFYVDPKVCIGCRTCVKTNCPPIRMKEYQGIDKL
                     KSFIDPDMCVGCSVCAQVCPVGAIKRSR"
BASE COUNT          848 a          990 c          771 g         1132 t
ORIGIN      
        1 aacaatgggg gccccgtacc tgaaaagtct cacggcctga ttgaatccct ccggtccaaa
       61 gcctggtccg aacaacttta caataaaggg tgcaaggaga aagttaagga ccatcaggat
      121 cagtcctagg attgtccaat aggaaatcag gttgtgggtg aactcaaacc ttcccttttt
      181 tccgtctctc cggtcaattt ggtggtagat gggaataagg gaaacaatta aggcatcggc
      241 cactattcca atcaggaaaa ccgatgctga aaatgccaca aggtaggtgt ctgtcctgaa
      301 attggctcca atgaaaaatg caagtaggat accctttagg aaattcaaga gccttgcaaa
      361 cagtccgaag gtcaggaggt cttgtgtctg tttttttatg gttttaaagg acatcatggc
      421 aattgctcct tttagtctgt attacaggac ttcccaggaa gtctaggctt tcctgggaag
      481 aaatagtgtt ccttttttct ctcctgctga tatctgggtt atgatatcct tggtttttcc
      541 gtgtgccact atcattggta ttccatagga attgaccatt ctggcagcct taagctttgt
      601 cttgataccg ccggttccaa aggagctggt ctctccgata gatatctcct tctccagctt
      661 gtctatatcc tcaactacat ccaccagaca tgcctggggg ttgtcatttg ggttgcagtc
      721 atatatgccg tcaatgtcgc tgaggataag gaggaggtct cctccccaaa caacggtggt
      781 atatgctcca agcatgtcgt tgtctcctat ttttatctca tctacgctta cggtgtcatt
      841 ttcatttaca attgggacca caccctcatc aatcagggtg aaaagtgtgt tcctaatgtt
      901 aagtgtcctt tccttgtgct cgaaatcatc ctttgtcaag agtatctgac cgatatggat
      961 cccatgcttg ttgaatatcc ttctgtagga gtccatcagc tcaacctggc cgattgagca
     1021 aagggcctgc ttgtagttta tgtcctgctg tcttgaccac ttgccgattg ttgaaactcc
     1081 cgcaattctt gctcctgatg aaacaattat cacctttttc ccctggtcca taagggtctt
     1141 gacctggagg atgaagttat caagaaaatc cttgtcaatg gtcccatcct ggcttgtaat
     1201 ggtgctggac cccaccttaa taacgatctt gttcgcattc cttatcatca tatcgtccat
     1261 ctcatcccta ctttctggtt tgtccctgtc ccattacaag gtatttgtgg gtaacaagct
     1321 gttcaagtcc aacaggtccc ctggcatggg tcttctgggt gctaatgccc atctcggctc
     1381 cgaatccgaa ttctcccccg tcggtaaacc tggtggatgc gttcacatat actgttgatg
     1441 catcgacctg tcgctggaac ttaagggcat tgcccagggt ctcggttatt atggattccg
     1501 agtgctttgt gccgtatttg ttgatgtggg ctatggcctc atcaacatcc tccaccacct
     1561 tgatggcaag gatgaagtca agaaattcag ttccccagtc ctcttcagtc gcagccttgg
     1621 cagatattat ctccctggtc tcttcatccg ccctaagctc aaccttgtcc ttgagggcct
     1681 catatgccat aggcaagaag tccttggcca ccttcctgtt cacaagtagg gtttcgcagg
     1741 cattgcagac cccaaccctt tgtgtctttg catttacaac aatcttaact ccgtcatgga
     1801 ggtttgccgt atcgtcaata tatacatggc agtttccctc acctgtctga agtgttggaa
     1861 ccgttgcatt cttaaccaca aaatcgatca gggcaccgct tccccttgga attacaaggt
     1921 caagaaaatc attgctcctt agcatctgga gcaccaggtc cctgtcggta tcgtcaataa
     1981 actcaaccac gccctcagga atgtctgtct tcttaagccc ctccttgatt gccttcacca
     2041 gggctacatt tgagtgtatg gcagtggagc ttccccttag gagtattgca tttccggatt
     2101 tcagggccag accaaaggca tccactgtga cgttgggcct tgactcatag acaattccga
     2161 tcactcccag aggcactgtc atctttgata tggtcagtcc gttttccagg gtccaaaccc
     2221 tgtctgattt ccatattgga tcgggaagct tgatcaccgt ataaagactt tcaatgattc
     2281 catcaatcct tccgtggtcc agtagaagcc tgtcaaccag accctccttc attcctgact
     2341 ttcttgcgtt ttctacatcc agtgcattgg ccttgagaat ttcctccctg ttatggtcta
     2401 tggaatgtgc aacctccctt agggccaggt ttttctcatg ggttgaagag acagcaagct
     2461 ttctctcaga ttccaccagc aatgcgccct tctcctgtaa actgatcatt tcttcccctc
     2521 ctttttctca aaaaataatt aacctttatt atatcagaaa atttaaataa tgaaaacaac
     2581 ttatggtcta gtcctcccca tactcatacc caatattgaa ggctttaata tttgcatcct
     2641 tggcctttgg agggacattt tctaggagta cctcctccca tatttccttt tctatgtcca
     2701 tatgccttgc aagtattcca aggacaaccg tatttgcagc cttgatattt cctgcatcct
     2761 tggcctcctt tacagcatcc acacggatga ccacatagtc cttttccagg tcctctatct
     2821 ccttttcagg gtatttcctc tgctcaagca ggacagagga tgggtaggtc cttttttcat
     2881 ttatcacaac aaccgcccct tttttcagga gccccttcca tcttagggcc tccaggggct
     2941 ccattccaag gagtatgtcc ccttctccag ctggaatgtt tggagaatgg accacatctc
     3001 cgattcttgc ggacccccag accattccgc ccctttgtga gagacccaca acatcgttgg
     3061 tcttcacatc cagtccagcc ttaagggccg cttcggcaat tatctttgtg gcaaggacaa
     3121 gtccctgtcc tcctacacct gcaaccatga tatttttcat ctgatgtcca cctcctatct
     3181 ggatctcttt attgcgccta caggacacac ctgggcacat acactacagc ctacacacat
     3241 gtcagggtca ataaaggact tgagcttgtc tataccctgg tattccttca tccttattgg
     3301 tggacagttt gtcttcacac aggtcctgca gcctatgcag acctttggat ccacatagaa
     3361 gttcttttcc tttatcttga acctcagggc gcagggcctt gttgtgatta taaccgatgt
     3421 acccttcctg cctgtttcct ccttcactgc ctccttcatg gccttgtagt caaactggtc
     3481 aacctcccgg acatttgtaa agccaattgc ccctagcatg gcccttatgt ccaccttcat
     3541 atcgtcagcc tggttgaagt ttccactgga ggcattgtgc tggccgcctg tcatggcagt
     3601 ggttgagttt tccaggatta tgaaggtcat attgtcatcc tcgtccatct ggtggaggag
     3661 atttacaaag ccggtcatgc cggagtggaa aaaggttcca tcacctatca ctgaaacaag
     3721 aggagcttcg tcaccgtttc t
//
LOCUS       DASRQF010000208         3020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_67506, whole genome shotgun sequence.
ACCESSION   DASRQF010000208 DASRQF010000000
VERSION     DASRQF010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67506"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1909)
                     /locus_tag="VFD79_05515"
     CDS             complement(<1..1909)
                     /locus_tag="VFD79_05515"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZH93542.1"
                     /translation="MNYNYNSSKTETPENLKERLEYYNSLRTSIRPGYESLRLIFIYV
                     ILGSFWIIFSDRILTYITGDLALYGRMQTFKGWFYVFFSGLVFYFIVKNRMEQFKNAT
                     DELYKGYMKLDMSHGELHEAKDQLQDRFDQLNYYKNALEESQLKYDLSIQGANDGVWS
                     WDIRNDNYNTSIMTKVNFGYSREDQESTDTMSKWQAYVHPEDLPKGKKRLEEYIENGN
                     DVYENIFRVRTANEDYRWILSKGKVIRGENGEPLRLAGSHTDLTEQMLLKENLHQEKE
                     LIESISKEAPLMILVCNRMGIVTKINSFGETLLGYEEDEILGKDAYEILIDEHDKSEL
                     DRHLEKVFQGKAMTNVETKIATKTGEIKTILWNNSFLHDMKGEIEGLVFVGVDISNRK
                     EMEKKLERLAYFDPLTDLYNREKFKEDTFSLIINNKGKDKLFGLVYLDVDNFKLINDT
                     MGHAVGDKLLRHIGDMMIHTVGKDDRVYRFGGDEFVMLVHDVPEENLISKIEALMDKI
                     RIPWKHNDQTFHISASAGVAIYPEHGEDYEQLMQNADTALSFAKDRGKNTYALYDREM
                     REKAWNHIQLSNDIREAIQKNQFELNYQPQIDLVTERIVGVEALIRWNHPAKGRISPV
                     DFIPFSEKTGQI"
     gene            complement(1996..2877)
                     /locus_tag="VFD79_05520"
     CDS             complement(1996..2877)
                     /locus_tag="VFD79_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZH93543.1"
                     /translation="MKIKLDPYRVFNQVALDKSISKASRKLFISQPAVSQVISQLESD
                     LKVRLFTRTPKGVVLTKEGELLFEYVNAGINLIEKGEKRLNDSKNLYEGELRIGVGDT
                     IARYYLIDYLERFNKFYPQVKLRIINRTTMELANLLKSGEIDIAVLNLPIGDNSFEII
                     PTSKIQDVLVAGQKYSYLKDRVLDLEELERVPLVMLDNRSKSREYVEGFLNTQGLKVS
                     PEIEIGSHDLLLELARIGLGLAFVVKEYSSQYMENGDVFQVETSFEIPARNVGICHLK
                     DVSMTPAAEKFVKILLG"
BASE COUNT          787 a          718 c          497 g         1018 t
ORIGIN      
        1 ctatctgccc cgtcttctca gaaaatggta tgaaatccac aggcgaaatc cttccctttg
       61 ctggatggtt ccatcttatt aaggcttcaa ctccaacaat cctctccgtg accaggtcaa
      121 tttgaggctg gtagttaagc tcaaactgat ttttttgtat tgcttccctg atatcattgc
      181 tcaattgtat atggttccag gctttttccc tcatttccct gtcatagaga gcatatgtgt
      241 tcttacctct gtcctttgca aaggacaagg cagtgtcggc attttgcatt agctgctcgt
      301 agtcctcccc atgttcaggg tagattgcaa ctcccgcact ggctgaaata tggaaggttt
      361 gatcattatg cttccatgga attcttatct tatccattaa agcttcaatt ttcgaaataa
      421 ggttttcctc aggcacatcg tggaccagca ttacaaattc gtccccacca aacctgtata
      481 ctctatcatc cttaccgacc gtatggatca tcatgtctcc aatatgtcta agtagcttgt
      541 ctcctacagc atgtcccatt gtatcattaa tcagcttaaa gttatcaaca tcaagataaa
      601 ctagtccgaa caatttgtct ttgcctttat tgttgatgat caggctaaat gtgtcctcct
      661 tgaatttctc cctattgtat aggtcggtca agggatcgaa ataagctagc ctttccagct
      721 tcttttccat ttcctttcta ttgcttatat ccactcccac gaaaaccagg ccttcaatct
      781 cacccttcat atcatgcaga aagctattgt tccaaagaat agtcttgatc tcacctgtct
      841 tggttgcaat ctttgtttca acattggtca tggccttgcc ttggaacacc ttctcgagat
      901 gtctgtccaa ctcacttttg tcatgctcat ctattaatat ttcatatgca tcctttccca
      961 atatctcatc ctcttcatat ccaagcaaag tttcaccaaa actgtttatc tttgtaacta
     1021 ttcccatcct gttacagaca agtatcatca gtggggcttc ctttgatatg ctttcaataa
     1081 gctccttttc ctggtgtagg ttctccttta ggagcatctg ttccgtcaaa tcagtatgag
     1141 agcctgccaa tctcagaggt tcaccatttt cgcctcttat tacctttcct ttgcttaaaa
     1201 tccatctgta atcttcattt gctgtcctaa ccctgaagat gttctcatag acatcgtttc
     1261 cattttcaat gtattcctcc agccgtttct tgcccttggg caagtcctcc ggatgtacgt
     1321 aggcctgcca tttggacatg gtatcagtag actcctggtc ttccctgcta tacccaaaat
     1381 tgacttttgt cattatggat gtgttgtagt tgtcatttct gatatcccaa ctccaaactc
     1441 catcatttgc tccctgtatt gacaggtcat atttcagttg actctcttcc agtgcattct
     1501 tatagtaatt caattggtca aacctatcct gaagctgatc ctttgcctca tgaagctccc
     1561 catgtgacat gtcaagcttc atgtagcctt tgtatagctc gtctgtggca ttcttgaact
     1621 gttccatcct attctttaca ataaagtaga atacaagtcc agaaaaaaag acgtagaacc
     1681 aacccttgaa tgtctgcatc cttccataga gagcaaggtc tcctgtaatg tacgtaagaa
     1741 ttctgtctga gaaaataatc caaaaggacc ccaaaataac ataaatgaaa ataagcctca
     1801 ggctttcata accgggtctt atactggtcc tgagactgtt atagtactcc agtctctctt
     1861 ttaaattttc tggtgtctct gtttttgaag aattgtagtt ataattcatg agagcctcca
     1921 tcaaatatct ttaactactt atacccatat cagccaatca atcacattta atagatgtct
     1981 gtcaatccaa ataattcagc cgagtagaat cttcacaaac ttttcagcag caggcgtcat
     2041 gcttacatcc ttaaggtggc agatcccgac atttctggct gggatttcaa aacttgtttc
     2101 aacttggaat acgtccccat tttccatata ctggcttgaa tactccttga ctacaaatgc
     2161 caagccaagc cctatcctcg ccagttcaag aagcaggtca tgggagccaa tctcaatttc
     2221 tggtgatacc ttaagacctt gtgtatttag gaaaccttca acatactccc tggattttga
     2281 tctgttgtca agcatcacca aggggaccct ctccagttcc tccaaatcca ataccctgtc
     2341 cttaaggtat gaatactttt gtcctgccac aagtacatct tgaattttgg aggttggtat
     2401 tatttcaaaa ctgttatctc caatgggcaa gttaagcaca gcaatatcta tttctcccga
     2461 tttcaataag tttgcaagct ccatggtagt tctgtttatt attcgtagct ttacctgagg
     2521 gtagaacttg ttgaatcttt caagataatc tatgagataa tatctggcaa tggtgtctcc
     2581 gactccaatc ctaagctccc cttcataaag gtttttactg tcattcagtc ttttttcccc
     2641 tttttcaatc aggtttatcc cagcattgac gtattcgaag agtagctctc cctccttggt
     2701 aagtactaca ccctttggag tcctggtaaa aagcctgacc tttagatcac tttccagctg
     2761 tgagattacc tgacttactg caggctgact gataaatagc tttcttgaag cttttgaaat
     2821 actcttatcc aaagccacct gattaaaaac tcgatagggg tctagcttaa tcttcatata
     2881 actcctcctt atctcccata tacttattat ttattttact tatatcataa aataaaatat
     2941 acttaatgta aagtgatgaa tatccaagat taaaaggaga gatgctttgt ggaaagaatg
     3001 gtaggtactg tagtcagagg
//
LOCUS       DASRQF010000209         3929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_773921, whole genome shotgun sequence.
ACCESSION   DASRQF010000209 DASRQF010000000
VERSION     DASRQF010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773921"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VFD79_05525"
     CDS             complement(<1..379)
                     /locus_tag="VFD79_05525"
                     /inference="COORDINATES: protein motif:HMM:NF028468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HZH93544.1"
                     /translation="MGSRGSDYADKLKLYKGIVLVSFLSLLLYFTAGFYNDAFMGVVS
                     VTNYLTWHLIFEFASVLVSFSIFTVTYFVYKESRRLSMIVLGCAFLLMGTLDAFHTFS
                     FKGMPDFFIQACRNRWNPGKHGSD"
     gene            complement(588..2045)
                     /locus_tag="VFD79_05530"
     CDS             complement(588..2045)
                     /locus_tag="VFD79_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016117154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HZH93545.1"
                     /translation="MGPIVDFLNAVIWSNALVYLLLGTGLYFSLRTRFVQVRLIKKMV
                     ALLFGNVASETGVSSFQAFALAVAGRVGTGNIAGVATAIALGGPGAVMWMWIIAFLGA
                     SSAYVEAALAQVYKDEVDEQYRGGPQYFIEKGLGIKWYAIAFSVATVVAMGFFLPGIQ
                     SNAIAVSMQNAFGIGPAITGGFIVLLLGLIIFGGVKRIGRVAEKVVPIMAIIYILVAL
                     AMMLFNLSEIPRVFGIIIGSAFGAEQAFAGIIGAAISMGVKRGIYSNEAGQGTGPMAA
                     AAAEVDHPAEQGLVQAFSVYFDTLFVCSATAFMIIISGMYNVYDGDAIIFEGAPGVEY
                     GPAFTQHAVDSFIPGFGLSFVAVALFFFAFTTLLAYGYYAETGVAYLFKNGKTRHTVI
                     TVTRLVFLGSVFAGTLSTVGEVWALGDIGVGLMAWLNVIAILLLSKTGLLTLKDYEDQ
                     LKAGVKVPTFDPDKLGIKNAETWKGINARKAKQNV"
     gene            2350..2724
                     /locus_tag="VFD79_05535"
     CDS             2350..2724
                     /locus_tag="VFD79_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HZH93546.1"
                     /translation="MNRISIKRIYEPITEDDGFRVLVDRLWPRGISKEKAGLDLWAKT
                     VAPSNELRKEYHKDLEQFEVFKGKYQNELNENKESVEFLDTINIKLEEGNVTFLTATK
                     DMDRNHVTVLVEWMDVMNQLRK"
     gene            2899..3129
                     /locus_tag="VFD79_05540"
     CDS             2899..3129
                     /locus_tag="VFD79_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HZH93547.1"
                     /translation="MKVQVKLFATFRNGRQELQEFEYPQDIPISTVLKDLSLSQSDVG
                     MALVNGIHATTDNKLHDHDVLALVPEYRQRHH"
     gene            3375..>3929
                     /locus_tag="VFD79_05545"
     CDS             3375..>3929
                     /locus_tag="VFD79_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93548.1"
                     /translation="MIKKAILILMVVGLMMGCSQVESSEFEEDEIVEEAQVEIVEEVD
                     KGQAQEENQLDDPVDPREAIDLSLEPNESGRIMVLMYHNIGEEEEEWTRTPDNFIKDL
                     QVLYEKGYRPISLKDYASGNITTPQGYTPIVITFDDGNKNNFEYMEDGSLNPDSAVGL
                     LMDFHKKNPDFSPAATFFLTGDIPF"
BASE COUNT         1307 a          765 c          783 g         1074 t
ORIGIN      
        1 tgtcgcttcc atgcttccca gggttccacc tgtttctgca ggcttgtata aagaagtctg
       61 gcatgccctt aaagctgaag gtgtggaatg catcaagagt acccattaaa agaaaagcgc
      121 agcctaaaac tatcatgctc aatcttctgg attccttgta aacaaaatat gtaactgtga
      181 agatggaaaa tgataccaaa acactggcaa actcgaatat aaggtgccag gtgagatagt
      241 tggtcacaga gacaactccc ataaaagcat cattgtagaa ccctgcagta aaatatagca
      301 acaaggaaag gaaagataca aggactattc ctttgtatag tttcaattta tctgcatagt
      361 cactccctct ggaacccatg gaatccctcc cttatatatg gttgatactg tgtatttact
      421 ttaaatagct ttattgcaat atttcaacac tccttgtaat tatataagag cttaaggaat
      481 ctatcaagat attttctaat aaagatgacg ccacctatca tttgataagt ggcgtcatct
      541 ttattagtat atcatgtaat tacacatata attcaataag tgtcaactta tacgttttgc
      601 ttagcctttc tagcgttaat gcccttccaa gtttcagcat tcttaattcc aagtttatcg
      661 ggatcgaaag taggcacttt tacgcctgct ttaagttgat cttcataatc cttcaaggta
      721 aggagacctg tttttgaaag gagaagtatg gctattacgt tcaaccatgc catcagtcca
      781 actcctatat caccaagagc ccaaacctct ccaactgtac ttagggttcc agcaaatact
      841 gatcctagga atactagtct tgtaacagtg attactgtgt gtctggtttt accattcttg
      901 aacaagtaag ctacacctgt ttctgcatag tatccataag caagaagtgt tgtaaatgca
      961 aagaagaata gagctactgc aacaaatgat agtccaaagc ctggaatgaa ggagtcaaca
     1021 gcatgctgag tgaaggcagg cccatattca acccctggag ctccttcaaa tatgatagca
     1081 tctccgtcat aaacattgta cattcctgaa ataataatca taaacgctgt agctgaacaa
     1141 acgaatagtg tatcaaagta aactgagaag gcctgtacaa gaccctgttc tgctgggtgg
     1201 tcaacctctg ctgcagctgc agccataggt ccagtaccct gtccagcttc gtttgagtag
     1261 ataccacgct taactcccat tgatattgca gcaccgatga tacctgcaaa tgcttgttct
     1321 gcaccgaaag cactgccgat aataatacca aatacccttg gtatttctga aagattaaac
     1381 aacatcatgg ccaatgcaac aaggatgtat atgatcgcca tgatgggaac tactttttct
     1441 gctactctac ctatacgctt tacaccaccg aatattataa gtcctaataa taaaactatg
     1501 aatccaccag ttattgccgg tccaatgccg aaagcgtttt gcatactaac tgctatagca
     1561 tttgattgaa tacctggtag gaagaatccc attgcaacta cagtcgcaac agaaaaagca
     1621 atcgcatacc attttatgcc aagacccttt tcgatgaagt actgtggtcc tccacgatac
     1681 tgctcatcga cctcatcctt gtatacctgt gccaaggcag cctcaacata cgctgaactt
     1741 gctccaagga atgcgattat ccacatccac attaccgctc ctggtcctcc caaagcaata
     1801 gcagttgcaa caccggcgat attaccggta ccaacacgac ctgctacggc cagagcgaaa
     1861 gcctggaatg aagatacacc tgtctcagac gctacgttac caaatagtaa tgcaaccatt
     1921 tttttaataa gtcgtacctg tacaaatcgg gttcttaatg aaaagtataa acctgtacca
     1981 agaagtagat aaactaatgc attactccaa atgactgcgt ttaaaaaatc aacaattggt
     2041 cccattcact aacctcctta ttttttgatt tatatgtgaa ttacatgaac aaattatact
     2101 ataaatgttt atgtaaaggc aagcgatttc atagaaaatt ctcaacattg tgaatagtta
     2161 attatataac aagaataata caaaatgaat tgctgaaaaa tacaaaagtg attacacaaa
     2221 aaatagaaaa atcattgatt ttagagattc tatcatcaaa tgatgatatt attgtatggg
     2281 gtagatgatc ttgtggtggg attaaaccag tacaaatatc gccgatatat caatggggag
     2341 tgagaagtca tgaataggat cagtatcaaa agaatttatg aacctatcac agaggatgat
     2401 gggttcaggg ttttggttga caggctatgg ccaaggggca tctctaagga aaaggctggt
     2461 ttggatcttt gggccaagac cgtggctccg tcaaatgagc ttaggaagga atatcacaag
     2521 gatctggaac aatttgaggt cttcaaggga aaataccaga atgaattgaa tgaaaacaaa
     2581 gaatctgtgg aatttcttga taccataaat atcaagctgg aagaaggtaa tgttacattt
     2641 ctcacagcca ccaaagatat ggataggaat catgtaacag tcttggttga gtggatggat
     2701 gttatgaacc agttgcgtaa gtaaaatcat cataaagcat taagcctcta tgcgtgataa
     2761 gaattgtgac atgaatgaga gtaaacttca caagaattac gatctatcga aaaacatgaa
     2821 attatattgg aaaatgaatt gcaagcatat gtcaagaatg atataataat atcaaacaaa
     2881 ggaaaggtga tgagcgctat gaaagtacaa gtcaagctgt ttgccacctt caggaacgga
     2941 agacaggagt tgcaggaatt tgaataccca caggatatac ctatctccac agtactgaaa
     3001 gacctgtcat tgtcacagag tgatgttggc atggctctgg tgaacgggat tcacgcaaca
     3061 actgacaaca agctgcacga ccatgatgta ttggctttgg tacctgaata cagacaaaga
     3121 caccattaag gggagcttta aagccaggcc atcaggaggg aatagagcgg ctactttatt
     3181 tccaaaatca attatggata caaaccttga ttttttagtg tttgtatctt tttttgtggg
     3241 catgggatat aattgacagt ttagcattaa tctgccttgt tacaaataac cctccatggt
     3301 acaataaaag ccaaggatta atacttgatg cacaaagact agatccttaa taacaatgaa
     3361 atgagtgtat tttgatgata aaaaaagcaa ttcttatatt gatggttgta ggtttgatga
     3421 tgggttgttc acaggttgaa tccagtgaat ttgaagagga cgaaatagtt gaggaagctc
     3481 aagtcgagat tgttgaagag gtggataagg gtcaggcgca ggaggagaac cagttggatg
     3541 accctgttga tcccagagaa gcaatagacc tttcactgga gcccaatgaa tctggaagaa
     3601 tcatggtgtt gatgtaccac aacatagggg aggaagagga ggagtggaca agaacaccag
     3661 ataacttcat aaaagacctt caggtccttt atgaaaaggg ttacagacct atcagcctaa
     3721 aggactacgc cagtggaaac atcacaaccc ctcaaggtta taccccaatt gtaataacct
     3781 ttgatgacgg caacaagaat aatttcgaat atatggagga cgggtcccta aacccggatt
     3841 cagcagttgg tctcttgatg gactttcata agaaaaatcc agacttttca cctgctgcaa
     3901 cattctttct tacaggggat attccattt
//
LOCUS       DASRQF010000210         5177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_129129, whole genome shotgun sequence.
ACCESSION   DASRQF010000210 DASRQF010000000
VERSION     DASRQF010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129129"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VFD79_05550"
     CDS             complement(<1..577)
                     /locus_tag="VFD79_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004832917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HZH93549.1"
                     /translation="MIIELKDVDYIYNPGTPFEKRALDKINLQVEEGEFIGLIGHTGS
                     GKSTLVQHLNGLMKPTKGTIIIDGEDLSSKETKMKSVRQKVGLVFQYPEHQLFEETIY
                     KDIAFGPKNLGLSDEEVDKRVRDAMELVGLEFEELKERSPFELSGGQKRRVAIAGVIA
                     MKPKVLILDEPTAGLDPKGRDDILDEIRKIYD"
     gene            complement(574..1419)
                     /locus_tag="VFD79_05555"
     CDS             complement(574..1419)
                     /locus_tag="VFD79_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HZH93550.1"
                     /translation="MNEKNNNIMIDIQNVSYEYSSYIDESIQLAVNDLSIQIKRGEFF
                     VVLGHNGSGKSTLAKMINGLILPSKGDIWVNGMNTRDEDKIWDIRSTAGMVFQNPDNQ
                     IVATIVEEDVAFGPENLGVPPAEIRRRVDDALEVVEMTEYKSHAPHLLSGGQKQRVAI
                     AGILAMEPDCIIFDEPTAMLDPSGRLEVMETIKKLNEDKNKTIILITHYMDEAVEADR
                     VMVLNEGEAVMLGTPKEIFKQVEKLKSLGLDVPQVTELVYELNKEGYNIKDDILSVEE
                     LVDTL"
     gene            complement(1557..1898)
                     /gene="rplQ"
                     /locus_tag="VFD79_05560"
     CDS             complement(1557..1898)
                     /gene="rplQ"
                     /locus_tag="VFD79_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005957415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HZH93551.1"
                     /translation="MTTLRKLGRPTAHRKAMLRNQVTSLLREERIETTVTRAKETKRM
                     AEKMITLAKKGDLHARRQALAFIYDEDVVRKLFDEIGPKYADRNGGYTRVLKVGPRRG
                     DGAEIAILELV"
     gene            complement(1923..2870)
                     /locus_tag="VFD79_05565"
     CDS             complement(1923..2870)
                     /locus_tag="VFD79_05565"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HZH93552.1"
                     /translation="MIEIEKPRIEIMELSEDGSYGKFVVEPLERGYGTTLGNSLRRVL
                     LSSLPGAAISSIKIQGVLHEFATIPGVLEDVPEIILNIKEVAAKMYTEEPVTMIIDVE
                     GPQVVTAGDIATGMEVEIVNKDQHIATVNEDGKLYIELELIRGRGYSVAEKNKKEGQP
                     VGVIPVDSSFTPVSKVNYQVGNTRVGQITDYDRLELDVWTNGTMSADEATSLGAKILT
                     EHLNLFIGLTDHVNEVEIMVEKEEDKKEKVLEMTVEELDLSVRSYNCLKRAGINTVEE
                     LTEKTEEDMMKVRNLGKKSLEEVQKKLAELGLGLKSSDE"
     gene            complement(2937..3560)
                     /gene="rpsD"
                     /locus_tag="VFD79_05570"
     CDS             complement(2937..3560)
                     /gene="rpsD"
                     /locus_tag="VFD79_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HZH93553.1"
                     /translation="MARYTGPVCRLCRREGTKLFLKGDRCYTDRCALNKRNFAPGQHG
                     QSRKKVTEYGMQLREKQKVRRFYGLSESQMSKYFMMADKMKGVTGENLFKILESRLDN
                     VVYRMGLAASRAEARQLVTHGHFLLNGNKATIPSLMTAVGDVIEVKEKSRGSEKFKTL
                     VENHSGNTAPWINIDLEKLRGTIVAESTREDIDLPVEEHLIVELYSK"
     gene            complement(3579..3977)
                     /gene="rpsK"
                     /locus_tag="VFD79_05575"
     CDS             complement(3579..3977)
                     /gene="rpsK"
                     /locus_tag="VFD79_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004833590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HZH93554.1"
                     /translation="MAPKKGKATRVRRRERKNIERGQAHISSSFNNTMVTLTDTQGNV
                     ISWASAGQLGFRGSRKSTPYAAQQCAEEAARKAMEHGLKVVEVFVKGPGSGREAAIRS
                     LQASGLEVSMIKDVTPIPHNGCRPPKRRRV"
     gene            complement(3996..4358)
                     /gene="rpsM"
                     /locus_tag="VFD79_05580"
     CDS             complement(3996..4358)
                     /gene="rpsM"
                     /locus_tag="VFD79_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009221332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HZH93555.1"
                     /translation="MARIAGVDLPRDKRVEIGLTYIFGIGKTSSNEILAKAGVDKDTR
                     VKDLTEAEVSNLRAVIDTYHVEGDLRRDIALNIKRLKEINCYRGLRHKRGLPVRGQRT
                     KTNARTRKGPRRLVSKKK"
     gene            complement(4393..4506)
                     /gene="rpmJ"
                     /locus_tag="VFD79_05585"
     CDS             complement(4393..4506)
                     /gene="rpmJ"
                     /locus_tag="VFD79_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HZH93556.1"
                     /translation="MKVRPSVKKMCEKCKVIKRKGRVMVICENPKHKQKQG"
     gene            complement(4523..4741)
                     /gene="infA"
                     /locus_tag="VFD79_05590"
     CDS             complement(4523..4741)
                     /gene="infA"
                     /locus_tag="VFD79_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HZH93557.1"
                     /translation="MAKKDVIEVEGTVIDALPNAIFKVRLENGHEILAHISGKLRMHY
                     IRILPGDTVTVELSPYDLTRGRITWRNK"
     gene            complement(4771..5052)
                     /locus_tag="VFD79_05595"
     CDS             complement(4771..5052)
                     /locus_tag="VFD79_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing RNA-binding protein"
                     /protein_id="HZH93558.1"
                     /translation="MDSTSDISIGQVVKSRAGRDKGRIFLIYDIADDQFVYVVDGDLR
                     KLDRPKKKKIKHLIVFNTVMPEFKDKIENSVKINNSYIRKILEPFNKSF"
BASE COUNT         1208 a         1306 c          854 g         1809 t
ORIGIN      
        1 tgtcatatat ctttctgatt tcatcaagga tgtcatccct gcccttgggg tcaagaccag
       61 ctgttggctc gtcaagtatc agaacctttg gcttcatggc aattacccct gcaatggcaa
      121 cccttctctt ctgtccacct gaaagctcaa agggtgacct ctccttaagc tcttcaaact
      181 caagaccaac aagctccatt gcatccctga ccctcttgtc aacttcctca tcggatagtc
      241 caagattttt tggtccaaag gcaatgtcct tgtaaatggt ctcttcaaac agctggtgtt
      301 cagggtattg aaacacaaga cccaccttct gtctcacaga cttcatcttg gtctccttgc
      361 ttgaaagatc ctcaccatcg attatgatgg ttcctttggt aggcttcata agtccgttca
      421 agtgctgtac aagggttgac ttgccagagc ctgtatggcc aataagtcct atgaattccc
      481 cctcctccac ctgaaggttt atcttgtcca gggccctctt ttcaaatggt gtcccaggat
      541 tataaatata gtctacgtcc ttcagttcta taatcatagc gtgtccacca gctcctcaac
      601 agataatata tcgtctttga tattataccc ttccttgttc aactcataca caagctccgt
      661 tacctgtggc acgtcaagtc ccagggactt aagcttttca acctgcttga aaatctcctt
      721 tggggttcca agcattacag cctcaccttc atttagcacc atgaccctgt cggcttccac
      781 agcctcatcc atgtagtggg tgataagaat gatggtcttg ttcttatcct cattcaactt
      841 ctttatggtc tccattacct caagtctccc tgatgggtca agcattgctg taggttcgtc
      901 aaaaatgatg caatcaggct ccatggccaa tattcccgct atggcaaccc tttgcttctg
      961 tccacctgat aggaggtgtg gagcatgact cttgtattct gtcatttcaa caacctcaag
     1021 agcatcatca acccgccttc ttatttcagc agggggcaca cccaggttct ccggtccaaa
     1081 ggcaacatcc tcctcaacaa ttgtggctac aatctgattg tcgggatttt gaaacaccat
     1141 accggctgtt gatctgatgt cccagatttt atcctcatcc cttgtgttca tgccattaac
     1201 ccagatatca cccttggatg gcagaatcaa tccgttaatc atctttgcaa gagtggattt
     1261 tcctgaccca ttgtgtccaa gaaccacaaa aaattcccct cttttgattt ggatgctcag
     1321 gtcatttacg gccaattgga tgctttcatc aatgtaggag ctgtattcat agctcacatt
     1381 ttgtatatcg atcattatat tgttgttttt ttcgttcata ttatacctct gttaaaattt
     1441 tctattacca gtttaatata acacaagaaa aatgttattt caattgacct ttcctggcaa
     1501 cctagtggat ttcacaaaac taaagggact aagtgtcaac ttaatccccc tccctattaa
     1561 acaagttcca atatagctat ttctgcgcca tctccgcgac gaggaccaac ttttaatacc
     1621 cttgtataac caccatttct gtcggcatac ttaggaccta tttcatcaaa taatttcctc
     1681 accacatctt catcatatat gaatgcaagt gcttgtctcc ttgcatgaag atcgcctttc
     1741 tttgccaaag taatcatctt ctcagccatt ctctttgtct ctttagctct ggttactgtg
     1801 gtttcaattc tttcttccct tagtaatgat gtaacttgat ttctcaacat tgcttttcta
     1861 tgagcagtag ggcgaccaag ttttcttagt gttgtcacat ttatccctcc ttcttgcgta
     1921 ggttattcat cacttgattt aaggcctaat cccaattcag caagcttttt ctgtacctct
     1981 tcaagggatt tcttgcctag atttctaacc ttcatcatgt cttcctcagt tttttcagtc
     2041 aattcctcaa cagtattgat gcctgctctc ttaagacagt tatagcttct aactgaaaga
     2101 tccagctcct ccactgtcat ttcaagtacc ttctccttct tgtcctcttc cttttccacc
     2161 attatttcaa cctcgttgac atggtctgta agtccgataa acagattaag gtgctccgta
     2221 aggatcttgg ctcccaatga agtggcttca tcagcactca tggttccatt tgtccagaca
     2281 tcaagctcaa gtctgtcgta gtcagttatc tgaccgaccc ttgtgtttcc gacctggtag
     2341 ttgaccttgc ttacaggagt aaaggatgag tctacaggaa taactcccac aggctgacct
     2401 tccttcttgt tcttctcggc aactgagtag cctcttcctc tgataagctc aagttcaata
     2461 tacaacttcc catcttcatt gacggttgct atatgctggt ccttgttcac tatttcaact
     2521 tccatccccg ttgcaatgtc accggctgtt acgacctgag gtccctccac gtcgatgatc
     2581 attgttacag gctcttcagt gtacatcttt gctgctactt ccttgatatt gagtatgatt
     2641 tctggcacat cttccaaaac gcctgggatg gttgcaaact catgcaggac tccctggatc
     2701 ttaattgatg atattgctgc tccgggcaat gatgaaagca gtactcttct aagagagttt
     2761 ccaagtgttg ttccgtagcc tctttcaagg ggctccacaa caaatttacc atagcttcca
     2821 tcttcactta attccatgat ctcaattcta ggcttttcaa tttctatcat taagcataac
     2881 cctccttgat ttttttaatt tttaacggac gagggcaact gatactatta tatcgattat
     2941 ttagaatata gctcgacgat tagatgttct tcaactggaa gatcaatatc ctctctggtt
     3001 gattcagcta cgattgttcc tcttagtttt tcaagatcta tgtttatcca tggtgctgta
     3061 tttccactgt ggttttcaac caaagtcttg aatttctcgc taccacggct tttttcctta
     3121 acctcgataa catcacctac tgcagtcata agtgaaggta ttgtcgcctt atttccattc
     3181 agcaggaagt gtccatgtgt cacaagctgt cttgcttctg ctcttgaagc agccagaccc
     3241 attctgtaaa ctacattgtc aagtcttgac tcaagaatct tgaacagatt ctcaccggtt
     3301 acacccttca ttttatcagc catcatgaag tacttgctca tctgtgactc tgaaagtcca
     3361 tagaatctac gtaccttttg cttttctctt agctgcattc catactcagt aactttcttt
     3421 ctagattgtc cgtgttgtcc tggagcaaag tttctcttgt taagagcaca tctgtctgta
     3481 taacatctgt ctcccttaag gaaaagcttt gtaccttctc ttcggcacaa tctgcaaact
     3541 ggccctgtat atcttgccat ctctatttac acctcctatt aaactcttct tctcttcggt
     3601 ggtctacatc cattgtgtgg aattggagta acatccttga tcatgctaac ttcaagtccg
     3661 gatgcctgta gtgaccttat tgccgcttct cttcctgatc caggtccctt tacaaatacc
     3721 tcaacgacct taagtccatg ctccatagct tttcttgcag cttcctcagc acattgttgt
     3781 gcggcataag gagtggattt ccttgaccct ctgaatccaa gctgtccagc gcttgcccat
     3841 gatattacat taccttgggt atctgtaagt gttaccattg tattgttgaa ggatgaagaa
     3901 atatgagcct gacctctctc aatgttctta cgttctcttc ttcttacacg tgttgcttta
     3961 ccttttttag gtgccattaa gttcccctcc tttacctatt tcttcttgga tactaatctt
     4021 ctaggacctt ttctagtcct tgcatttgtc ttggttctct gtcctcttac tggaagtccc
     4081 ctcttgtgtc taagtcctct gtagcagttg atttccttaa gtcttttaat gtttagggcg
     4141 atatctcttc taagatctcc ctcaacatgg taggtatcaa tcactgccct aaggttggaa
     4201 acttcagcct cagttaggtc ctttacccta gtgtccttgt caactccagc ttttgccaga
     4261 atttcatttg agctggtctt tccaatacca aatatatatg tcaaaccaat ttctactctc
     4321 ttatctcttg gtaagtctac gcctgcaatt cttgccatta acgtctacac ctcctatttt
     4381 tcaaactcgt tattagcctt gtttttgctt atgtttagga ttttcacaga tgaccattac
     4441 tcttcctttt cttttaatga ctttgcattt ttcacacatc ttttttacag atggtctgac
     4501 tttcataata atccctccta tgctacttgt ttcgccaggt tatcctgcct cttgttaaat
     4561 cgtagggcga tagttctact gttacggtat ctcctggtag tatcctgatg tagtgcatcc
     4621 tcaacttacc ggaaatatgg gctaaaattt cgtgtccgtt ctcaagcctt actttaaata
     4681 tggcattagg taatgcatcg ataaccgtac cttcaacctc tattacatct tttttagcca
     4741 tggatttcga acctcctgtt caactctcat ttagaaactc ttattgaaag gttccagaat
     4801 ttttctaata tatgagttat ttatctttac gctattctct atcttgtcct tgaattccgg
     4861 cataacagtg ttgaatacaa tcaagtgctt gatttttttc ttctttggtc tgtcaagctt
     4921 cctaagatct ccgtcaacta catatacaaa ttgatcatca gctatgtcgt atatcaagaa
     4981 tattcttcct ttatcacggc cagctcttga cttcaccacc tgtccgatgg atatgtcact
     5041 agtggaatcc attatattca cctctttgtt ctgctggtaa gggtgttacc cccttatttg
     5101 tcaagttcgc ttgtgatatc aacgaaaacc tcttcaatct tcctggttcc atccacatca
     5161 agcagcaatc cttgatt
//
LOCUS       DASRQF010000211         3011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_620627, whole genome shotgun sequence.
ACCESSION   DASRQF010000211 DASRQF010000000
VERSION     DASRQF010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620627"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VFD79_05600"
     CDS             complement(<1..479)
                     /locus_tag="VFD79_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HZH93559.1"
                     /translation="MQKGKYGLTTKVLMALVIGIIVGILVYSLPSGTLKDTILVDGIF
                     LFLGQVFLRGIMMLVVPLVFISLVNGAANMGDITKLGRIGSKTILFYMTTTAIAIVIA
                     LTLGVLLKPGIGLDMGAVEAVDVAARDTVPLVQIFYELIPNNIIGAMAAGSMLQIIV"
     gene            complement(591..2021)
                     /gene="gatB"
                     /locus_tag="VFD79_05605"
     CDS             complement(591..2021)
                     /gene="gatB"
                     /locus_tag="VFD79_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HZH93560.1"
                     /translation="MAIKTLIGLEIHVELSTDKKMFCNCRNQYGADANTLVCPVCLGL
                     PGALPVVDKQAVEYALRAGLAFDCQIADVIRMDRKNYFYPDLTKGYQISQSDNPLCSG
                     GYLEIEPEAGPKRISLDRIHIEEDTGKQLHTEDGTSLLDFNRSGVPLVEIVSNPDMNS
                     SEEAKLFLEKLRNTLKYIGISDVRMEEGSLRCDVNINVVDQEKGTRTSITEVKNLNSF
                     RSAVKAIEYEEARHIRLLDEGESNVKETRRWDEVAGETVIMRRKYTADDYRFANEADI
                     TPIPVDREWVEEIRKSLPELPHAKKMRFMEDYSLSDYDAGVLTQSQDLAHFFEETLDH
                     VKDAKLLGNWMMGDVLRRLKDEDKDFDQMPISSRDLADLINLVKEDKINNNTGKKVLR
                     EMFETGKAPGDIVKEKGLIQISDQGELEKIVIETLDSNPQSIEDFKNGKDRAIGFLVG
                     QIMKATKGKANPQLVNKLLMEKIKER"
     gene            complement(2021..>3011)
                     /locus_tag="VFD79_05610"
     CDS             complement(2021..>3011)
                     /locus_tag="VFD79_05610"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HZH93561.1"
                     /translation="PGGSSGGSAAAVAGFEAPLALGSDTGGSVRQPASFCGIVGMKPT
                     YGMVSRYGVVPMSNSLDQIGVFGRDVKDAHMMLASVSGYDPLDATSSKKADRDISIEG
                     LDSLKDLRVALPEQYRNLRVTGDEKIQERFKETVKLFQDAGAIIDYVSLPSLEYSLET
                     YYIIMSSEVSTNLSRFDGVRFGYRTENYETLDELYTNSRTEAFGEETKRRIMLGTYSL
                     SAGYQTENYKKALRVRTLIKQDYENAFKDHDVILSLTAPMLPFEIDSMINSPVDMYKA
                     DLYTVPVNLAGLCAISVPMGDVDGLPVGLQITGPRFGDSTILKAGLGYERLVR"
BASE COUNT          714 a          797 c          616 g          884 t
ORIGIN      
        1 aaaactatga tctgcagcat gcttcccgct gccattgcac ctataatgtt atttggaatc
       61 agttcgtaga agatctgtac caggggcaca gtatcccttg cagctacatc cactgcttca
      121 acagctccca tgtcaagtcc aattcccggc tttaagagca ctccgagagt caatgcaatc
      181 actatggcaa ttgctgttgt ggtcatgtag aagaggattg tctttgaacc tatcctacca
      241 agctttgtga tatctcccat gtttgcagct ccattaacca gtgaaataaa taccaatgga
      301 acaacaagca tcatgatacc acgcaggaat acctgtccca ggaacaggaa tatcccatcc
      361 accaaaatgg tgtccttcaa ggttccagat ggcaatgaat atactaaaat acctacgata
      421 ataccgatta ccaaagccat taggaccttt gtggtgagtc catacttacc tttttgcata
      481 tattttcccc ctttccttat tatatccatt ataatggtat agcttaacaa agtcaaaaca
      541 tataaacagg gcagagactg gtctctgccc caaacaagca aaatatggaa ttatctttcc
      601 tttatttttt ccatcaacag cttgttcacc agctgtggat tggcctttcc cttggtggcc
      661 ttcatgatct gtcccacaag gaaacctatg gctctgtcct taccattctt gaagtcctca
      721 attgattgag gattgctgtc aagggtctca attactatct tctcaagctc cccttggtca
      781 gaaatctgga tcagaccctt ttccttgaca atgtctccag gtgcctttcc agtttcaaac
      841 atttccctca ataccttctt gcctgtgttg ttgtttatct tgtcttcctt cacaaggttt
      901 atcaggtcag caaggtccct tgatgatatg ggcatttggt caaaatcctt gtcctcgtcc
      961 ttcaatctcc tgaggacatc tcccatcatc cagttgccaa gaagcttggc gtccttaaca
     1021 tggtcaaggg tctcctcgaa gaagtgggcc aggtcctgtg actgggtgag gactcctgca
     1081 tcgtagtcgc ttaggctgta atcctccata aacctcattt tctttgcgtg gggcagctct
     1141 ggcagacttt ttcttatttc ctctacccat tccctgtcca cagggatcgg agttatgtcg
     1201 gcctcgttgg caaatctgta gtcatcagca gtgtacttcc ttctcatgat cactgtctct
     1261 ccagctactt catcccacct tctggtttcc ttcacattgg actcgccttc atccagaagc
     1321 cttatgtgtc tggcttcctc gtattcgatt gcctttactg cagacctgaa ggagttgagg
     1381 tttttgacct ctgttatgct ggtcctggtt cccttctcct ggtccacgac gtttatgttc
     1441 acgtcacacc ttagggaccc ttcctccatc cttacatctg aaatcccaat atacttaagg
     1501 gtgttcctaa gcttctcaag aaagagcttg gcctcctcag aggaattcat gtcaggattt
     1561 gagactatct ccacaagggg aactcccgac ctgttaaagt ccaaaaggga tgtcccatcc
     1621 tcagtatgaa gctgcttgcc cgtatcctcc tctatgtgga tcctgtccag gctgatcctc
     1681 ttggggcccg cctccggctc tatctcgaga tagcctccgg aacagagtgg attgtcggat
     1741 tgtgatatct ggtaaccttt ggtcaggtca ggatagaaat agttcttcct gtccatcctt
     1801 attacatctg ctatctggca gtcaaaggca agtcctgccc ttagggcata ctccacagcc
     1861 tgcttgtcaa caacaggcag ggcacctgga agcccgaggc ataccgggca cacaagggtg
     1921 ttggcatcag ctccgtattg gttcctgcaa ttgcagaaca tctttttgtc cgtggaaagc
     1981 tccacgtgaa tttccagacc tatcaaggtt ttaattgcca ttatctcacc aacctttcat
     2041 agccaagacc ggctttaagt attgtggaat ccccaaatct tggtccagtt atctgcagtc
     2101 caaccgggag tccgtcaaca tctcccattg gcactgaaat tgcacataat cctgcaaggt
     2161 tcaccggtac tgtataaagg tctgccttgt acatatcaac aggtgaattt atcattgaat
     2221 cgatttcaaa tggcagcatg ggtgcagtca gggacagtat cacatcgtgg tccttgaagg
     2281 cattctcgta atcctgcttg ataagggtcc taaccctcag agccttcttg tagttttcag
     2341 tctggtaacc ggcactcagt gaataggtcc caagcattat ccttctcttt gtttcctctc
     2401 cgaaggcctc ggtccttgag tttgtgtaaa gctcgtcaag tgtttcatag ttttcggtcc
     2461 tgtagccaaa acgaacccca tcaaatcttg aaaggtttgt actcacttcc gaggacatga
     2521 ttatatagta ggtttcaagg gaatattcaa gggatggcag ggaaacatag tcgataattg
     2581 ctcctgcatc ctggaatagc ttcaccgtct ccttgaatct ttcctgaatt ttttcatccc
     2641 cagtaacccg aaggtttctg tactgctcag gcaatgcgac ccttaggtcc ttcagggagt
     2701 caagaccctc tattgagatg tccctgtcgg cctttttgga tgatgtggca tccagtgggt
     2761 catatccgga aacacttgca agcatcatgt gggcatcctt tacatccctt ccaaagactc
     2821 caatctggtc aagtgagttg gacattggta caaccccata tcttgacacc attccgtagg
     2881 ttggcttcat cccaactatc ccacagaagc tggcaggctg tcttacagac cctccagtat
     2941 ccgaaccgag tgccaggggc gcctcaaatc ctgcaactgc ggcagcagac cctccggaag
     3001 aacctcctgg a
//
LOCUS       DASRQF010000212         9988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_289198, whole genome shotgun sequence.
ACCESSION   DASRQF010000212 DASRQF010000000
VERSION     DASRQF010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 9988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9988
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289198"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1826)
                     /locus_tag="VFD79_05615"
     CDS             complement(<1..1826)
                     /locus_tag="VFD79_05615"
                     /inference="COORDINATES: protein motif:HMM:NF019450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HZH93562.1"
                     /translation="MNRLMKLFSIGILAMILTMFNGCNTEATGMEPIRLVVDGKDITH
                     LTEPIIENGRTLVPIRFVTEEIGGQVSWDNATRTVKAWKGERSLRMIIGSRVVELGNG
                     EDAVVSDVSPRIIEGRTFVPLRLISNGLGIDVSWDNSTRTVLVDSGKSAAVAPFYNVN
                     ITSHQGGSYITGTSRITVSGDSSLLKAGNQLRLLLLDKKDHTGYIVGMADAATRAVDY
                     IPEVENTGDRMLVAGVYDQNGTFLGGDSIPVAVRVIPEADMAGLVDNMLVSSAREISP
                     RLNFAPYSVKYVITNLSSGRVRVEENRDPYGPFTWNPMSTDNGLNTVQIIALDHNKKE
                     YPGRTYTVNVQVPKRITLSGVTEGSTIRNPVTLLANRNFDVRETTFLMRDPSTGVETV
                     LQTMPYGSYTWFPGPEISGWKDLMVRVIDSQGNTVTSEPVRVNLEGKPQVQLQGIGPK
                     QVVTGATDLTYRTNVDLSDLRFVLTNRGTGETRTINANPDTKKAVYTPLSKDTGNWRI
                     LARATYNGMIITSETVDFEVYAQETFGPYSIIAKDQFKSFAAQMAVDTYKRTGMSAAI
                     QTSQAILETGWGQYVPSDKYTNKKSENLFGIKGSASNGSVIS"
     gene            complement(1859..3034)
                     /locus_tag="VFD79_05620"
     CDS             complement(1859..3034)
                     /locus_tag="VFD79_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HZH93563.1"
                     /translation="MNNKDGKIDRLKEYIKRLNRKEDGRKLYLEYKDDITSVQPQDAF
                     QIFHGLLEEGMTEREILTFLDKIINVFHESLTGHTWKRPENDNFLTDLELENEALRKK
                     TDHIKTIVKEPTSQIRREKLIPLIEELKDFYPHYAKKENILFPYLEKNKARFEGLTIM
                     WALHDMVKDQVDNALKLLKDPESDDHEVNVAIAELFFGILGIVKKEEIILFPSACEAL
                     TEEEWHQMHRQSYDYSFPFIEKEWEEEILEAQEAVMDKGRFKTETGDLSFDQIKLIFD
                     SLPVDMTFVDQDNKVRFFTRPKDRIFPRSPAIIGRDVNKCHPPDSVHVVEEIVEAFRR
                     GDRDTATFWIRIKGRMILIQYFAMRNDKGEYRGVLEVSQDITEIQALEGERRLLEWS"
     gene            complement(3110..3661)
                     /locus_tag="VFD79_05625"
     CDS             complement(3110..3661)
                     /locus_tag="VFD79_05625"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HZH93564.1"
                     /translation="MPVLKTDRLLMRRFRLTDLNDFYEYAKDPVVGPNAGWDYHRTRD
                     ESLLLLKKFTKSNEIWAIEHVETSKVIGSIGIHRDRKRENKSARMIGYVINSKYWGKG
                     YATEAAKKIMEYAFDQLDIDLISAYHYSHNDRSKRVIRKCGMTHEGVLKCATVTYDGL
                     VYDEHCYSITKDEFNKVTIPTGV"
     gene            complement(3805..5571)
                     /locus_tag="VFD79_05630"
     CDS             complement(3805..5571)
                     /locus_tag="VFD79_05630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZH93565.1"
                     /translation="MNSIYHKLLVNAPMGFLILEGEGSFEEMVVHDFNTLLKDYLSLE
                     EPVSRMKIVDILEKGIAQQLGDVVEALREEGEVEGIVFINSFYFNIKGLDLGDGFFAI
                     TLTERAGYLDNYRSSEKTRLNLLENLPGMVYRCRLDENWTMEYVSEGCEDLTGYSVDS
                     LLGNRDISYNEIIDEKYRKDLFELWRRAVENRSMVKVEYEIVTSAGQKKWVYEQGQPV
                     FDKQGNLVVIEGIIVDISEQKEREERINYLTYYDAMTGVYNRRYYNNTIDEMDCEDSL
                     PLSVIVGDINGLKLINDAFGHEYGDQLIIRTADLLKESIRSTDVLARTGGDEFVIMLP
                     NTPMDIASRIVKRIGQEGEVINELNRDSPVQISISLGYATKETMEDNLSDTIKLAEDH
                     MYRNKLFDHKSTHGTIIKSIRNSLLRRGEETEENLTNMEKVAIKMGRRIGFNDEMIEK
                     LQTLVSIHDIGKIAIDTELLNKEEPLTKEEWDIIRKHPEMGYRIAMASLELAAISDYI
                     LSHHERWDGTGYPDGLKYDEIPVLSRIVNIIDSYDAMITERPYKKKLSQVEALREIER
                     NAGTQFDPDLAQVFVQVVREMD"
     gene            5782..6684
                     /locus_tag="VFD79_05635"
     CDS             5782..6684
                     /locus_tag="VFD79_05635"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZH93566.1"
                     /translation="MNLSKGKNSRKIILLMLVLALGVFAGACEKAEDPSDVEVVAKIN
                     DVEITKDEFYNYLLQQNGPEVLEALILEKMVMMEVEANNVVISEEAIQEELDVMKSSY
                     GGDEAFEEALVYYGFTEESVKKNIQLNLGIEALMEPYIDITEDEILQYFIANKAEFDT
                     SDQVNASHILVDTEEEARDIKQQLDDGGDFAELAAQYSKDASNAQQGGALGFFGKGRM
                     VAPFEAAAFSMEPGEISEPVQTDFGFHIIKVEDRSEGQEAQLEDVSEDIREILKDNKM
                     NSAYSVWYEDVQEKYDVENFILNN"
     gene            complement(6729..7280)
                     /locus_tag="VFD79_05640"
     CDS             complement(6729..7280)
                     /locus_tag="VFD79_05640"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZH93567.1"
                     /translation="MQLAEVKGLPHKVVKNIEGVDNLKVYLVQDFNLKLLRNMVEFGL
                     DIFGDLGMDQWGLVPQIRHGNVYILTEEGKHQVVGLAILMRDWEDFYKAYLFDYAISE
                     DYQSMGLGFEFLKIIAEDLLDQGFDKMSLTVDVENEPAIRLYRDKIGFKSILYSKNEY
                     GKGEDRFTMELDLAGFLERNKNK"
     gene            7447..8952
                     /locus_tag="VFD79_05645"
     CDS             7447..8952
                     /locus_tag="VFD79_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZH93568.1"
                     /translation="MEFKDKITRMMQILKITNSQLAERLDVDMSLVSRWRTGNRLPSQ
                     RGDHMEQIADYLMECAFQKDLLDEFTELLGLSADAHRNDHAYLRSHLLNWLQDEKFQD
                     NSPYIRNFIDKVEFFTENSNNTSHEKLHSVPKYSAKDIYLGTEGKREAMIRFLHQIIL
                     SDEILDIYFYSDESQEWISDDIDFYFTFNGMLSDILAKGHRLKIIQPYYRDFRSMMES
                     IERSLPIYISGQIQTYYYPQEMKSIFGNTVALAGDRAAIKSASIKSNSHNSINFYVDK
                     KDVVLSMKDNLLSFIGECDSLMSIFGFHNLKELIAYLLDQGDSPGDLIALHQTLSTMT
                     IPMEFLYMLSDSKDLHKAHIKRTDLLKDNLKKYKYIEIFSLPSLDELDAGKEQVELSN
                     HLSSDPIYYDRNTFRIHLENVIDWLETYPNYNVIILDKLFLKDVRITVKKDRTAILYK
                     MRPSPIAIAFDNKEIIEAFYSYIVSVINESNEKTLDRRLSIERLKDYMSKL"
     gene            9098..>9988
                     /locus_tag="VFD79_05650"
     CDS             9098..>9988
                     /locus_tag="VFD79_05650"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZH93569.1"
                     /translation="MEVNLNLGSNPGKDKPKMNPETTYDLIVLGGGPAGLNTALYAKR
                     KGLQVGVIARDIGGQVMDTSSVENYLGFTSLSGEELMKKFVGHVNKLEVPVLEYEELE
                     SVNVKDNSNIREVVLKNGQRFNTYSVVIATGSKPRKLGVPGEKEYSGKGVCYCAICDG
                     PLFAGEEVIVAGGGNSAVEAAIDLAKIAKKVTIVHRSQFRADQILIDQLSRLDNVEVK
                     LQTQILEVEGDKFMTGVRVADRESGEEYIVEGAGLFVEIGYLPNSEMFSNLLSLNERN
                     EIVVDRYGKTSVDGIYAAGDV"
BASE COUNT         2612 a         2337 c         2085 g         2954 t
ORIGIN      
        1 ttggagatta ctgatccgtt gcttgcacta ccctttattc caaagaggtt ttctgacttc
       61 ttgttcgtat acttgtcact tggcacatac tgaccccagc ctgtctcaag gattgcctgg
      121 gatgtctgga ttgccgccga cattccagtc cttttgtagg tatccactgc catctgggct
      181 gcaaaggatt tgaactggtc ctttgctatt atgctgtatg gaccgaaggt ctcctgtgca
      241 tagacctcaa aatcaacggt ttcacttgtt attatcatgc cgttataggt tgcccgcgca
      301 agaattctcc agtttcccgt atccttggac aagggagtgt agacggcctt ttttgtatcc
      361 ggattggcat ttatggttct ggtctcgcca gtgcccctgt ttgtaaggac aaaccgaagg
      421 tcagaaaggt caacattagt cctgtaggtc aggtctgtag cccctgtaac aacctgcttg
      481 ggtccgattc cctgaagctg gacctgtggc ttgccttcca ggttcactct tacaggctcg
      541 ctggttacgg tgtttccctg cgaatcaatt acccttacca taaggtcttt ccatccggat
      601 atttccggac ctggaaacca ggtgtaggat ccgtagggca tggtctgaag gactgtctcc
      661 acaccagttg acgggtccct cataaggaag gtggtttccc taacatcaaa gtttctgttt
      721 gccaggaggg tcaccggatt tctaatggtt gaaccctctg tcacccctga aagggtaatc
      781 ctttttggaa cctggacatt gactgtatag gtccttcccg gatattcctt cttgttgtgg
      841 tccagggcta tgatctgtac agtgttcagc ccgttgtcag tggacattgg gttccaggtg
      901 aagggtccgt atggatccct gttttcctcg accctgaccc ttccactgga aaggtttgtt
      961 attacatatt ttacagaata aggtgcaaag ttaagtcttg gactaatctc cctagctgag
     1021 cttaccagca tgttgtcaac aagacctgcc atatcagcct ctggaattac cctgaccgct
     1081 acaggtatgc tgtctcctcc caggaatgtt ccattctggt catagactcc tgcaacaagc
     1141 atcctgtccc ctgtattttc aacctcagga atgtaatcca cagcccttgt ggctgcatct
     1201 gccatcccaa cgatgtagcc agtatggtcc ttcttgtcca gaagcagcag tcttaactgg
     1261 ttgcctgcct taagaaggct tgaatctcca gacacggtta tccttgaggt ccctgttata
     1321 tagcttcctc cctggtggga tgtgatgttc acattgtaga agggtgcaac agctgcgctc
     1381 tttcccgagt ccacaagtac tgttcttgtg gaattgtccc agctaacatc tatgcctagg
     1441 ccattgctta tgagtcttag ggggacaaag gtcctgccct caattatcct tggagagaca
     1501 tcgcttacaa cagcatcctc accatttcca agctccacta ccctgcttcc tattatcatt
     1561 cttagggacc tttctccctt ccaggccttg acggtcctgg tggcattgtc ccagcttacc
     1621 tggccgccaa tctcctctgt gacaaatctg attggcacaa gggtccttcc attctcaatt
     1681 attggttcag taagatgggt gatgtccttt ccatccacca caagcctaat gggttccatc
     1741 cctgtggcct ctgtattgca gccattgaac attgttagga tcatggccag tataccaatt
     1801 gaaaaaagct tcattaatct attcatgtct gtgcacctct cttttgctat ttattgtgtc
     1861 agctccactc caaaagtctt ctttcccctt caagggcctg aatctctgta atatcctgac
     1921 taacctcaag gacgcccctg tattcaccct tgtcatttct catggcaaag tactggatca
     1981 ggatcatcct acccttaatc cttatccaaa aggtggcagt gtccctgtcc cctcttctaa
     2041 aggcctccac tatctcctca acaacatgga cgctgtcagg tggatggcac ttgttcacat
     2101 cccgcccgat gattgctggg gaccttggga atatcctgtc cttgggcctt gtaaagaacc
     2161 tgaccttgtt gtcttggtcc acaaaggtca tgtccacagg aagggaatca aatatcagct
     2221 taatctggtc aaaggacagg tctcctgtct ctgtcttaaa ccttcccttg tccattacgg
     2281 cctcctgggc ctccaggatt tcctcctccc attccttttc tataaatgga aagctgtaat
     2341 cgtagctttg tctgtgcatc tggtgccatt cctcctcggt cagggcctca caggctgagg
     2401 gaaacagtat tatttcctcc ttcttaacta tacccaatat tccaaagaag agctctgcta
     2461 ttgcaacatt gacttcatgg tcgtcgcttt caggatcctt gagcaacttc aaggcattgt
     2521 ccacctggtc cttgaccatg tcatggaggg cccacattat tgtcaggcct tcaaatcttg
     2581 ccttgttttt ttcaaggtac ggaaaaagta tattctcctt cttggcatag tgggggtaga
     2641 agtccttcaa ctcctcaata aggggaataa gcttttccct tcttatctgg gaggttggct
     2701 ccttcactat cgtctttata tggtctgtct tctttctcaa ggcttcattc tcaagctcca
     2761 ggtccgtgag gaagttgtcg ttttcaggtc tcttccaggt atggcctgtg aggctttcat
     2821 ggaatacgtt tataatcttg tccagaaaag tgagtatttc cctttcagtc attccctcct
     2881 ccaagagtcc atgaaaaatc tgaaaggcat cctggggttg gacacttgtg atatcgtcct
     2941 tgtactccag atagagcttt ctcccatcct ccttgcggtt tagtctcttg atgtattcct
     3001 tcaacctatc tatctttcca tccttattat tcatcccacc gctcccttca aagtgatgct
     3061 ttaattatac cttagaagct gccgcgaggc aaaaaaataa caggagcttt tacactcctg
     3121 tgggtattgt caccttattg aattcatctt ttgttatgga gtaacagtgc tcatcatata
     3181 ccaagccatc gtaggttacg gttgcacact tcagaacccc ttcatgggtc attccacact
     3241 ttcttatcac cctcttggat cggtcgttgt gggaatagtg gtaggcggat atcaggtcaa
     3301 tgtccagttg gtcaaaggca tattccatta ttttttttgc tgcctctgtg gcatagccct
     3361 taccccagta tttggaattt attacgtatc cgatcatcct tgctgatttg ttctcccttt
     3421 ttctgtccct atgtatccct attgacccaa ttaccttgga ggtctccaca tgctcaattg
     3481 cccatatttc attgctcttg gtaaacttct taagcaataa gagagattca tccctggtcc
     3541 tgtggtagtc ccatcctgcg ttaggtccta caacagggtc tttagcatat tcatagaaat
     3601 cgttaaggtc agtcagccta aaccttctca tgagtagtct gtctgttttg agtacgggca
     3661 tgctttcacc tctcattaca actttgtttt cataacaata ttttagcata ttatccaata
     3721 tctgggaata taagtgacac atttatttgc ataaatcaaa aataatcaaa ccaaggattt
     3781 ccattaggtc ggctctctag ggatttaatc catctccctg accacctgga caaatacctg
     3841 ggccaggtcc gggtcaaatt gggttccggc attcctctca atttccctta atgcttctac
     3901 ctgggacagc ttcttcttgt agggtcgctc tgttatcatg gcatcatagg agtctatgat
     3961 gttgactatt cttgatagga ccggaatttc atcgtatttc aaaccatcag gatagcccgt
     4021 accgtcccac ctttcatggt gggagaggat gtagtcggaa atagcagcaa gctccaggga
     4081 tgccattgca atcctgtagc ccatctcagg gtgctttctg atgatgtccc attcctcctt
     4141 ggtcaagggt tcctccttgt ttaaaagctc agtgtcaatt gctatcttcc ctatgtcgtg
     4201 tattgacacc agggtctgaa gcttttcaat catctcgtca ttgaagccaa ttcgtctgcc
     4261 catctttatg gcaaccttct ccatgttggt caggttctcc tcagtctcct ctcctcttct
     4321 gagaagtgaa tttctgattg acttgattat tgtaccgtgt gtgctcttgt ggtcgaagag
     4381 cttgtttctg tacatgtggt cctcggcaag cttgatggta tccgaaaggt tatcctccat
     4441 ggtctccttg gttgcatacc caagtgaaat gctgatctgt acgggagaat ccctgttcaa
     4501 ctcattaatg acctctccct cctgaccaat cctcttgact atcctgctgg caatgtccat
     4561 tggagtattt ggaagcataa ttacaaactc gtcaccaccg gttctggcaa gcacgtctgt
     4621 acttcttatg gattccttca aaaggtcggc agtccttatt atcagctggt ctccatactc
     4681 atggccaaag gcatcattta tgagcttgag cccatttata tcccccacta ttacagacag
     4741 ggggagtgaa tcctcacagt ccatctcatc tatggtattg ttgtagtacc tcctgttgta
     4801 taccccggtc atggcatcgt aataggtcag atagtttatc ctctcttccc tttccttctg
     4861 ctcgctaata tcaactatga tcccctcaat aaccaccaga ttaccctgct tgtcaaaaac
     4921 cggttggccc tgctcataaa cccatttctt ctgacctgct gaagttacaa tctcgtattc
     4981 caccttaacc atggacctgt tttccacagc cctcctccac agttcaaaca ggtctttcct
     5041 gtatttttca tctattatct cattgtagga gatatccctg ttgccaagaa gactgtcaac
     5101 tgaatatccc gtcaaatcct cacagccctc ggacacatac tccatggtcc agttttcatc
     5161 caacctgcac cggtatacca tacctggaag gttttccagc agattgagtc ttgtcttttc
     5221 agatgatctg taattgtcca aatagcctgc cctttcagtt agtgttattg caaagaatcc
     5281 atccccaagg tccaatccct tgatgttgaa gtaaaatgag tttatgaaaa ctatgccctc
     5341 aacctcaccc tcttctctta atgcttcaac aacatctccc agttgttggg ctatgccctt
     5401 ctccaaaata tctacaatct tcattctcga tacgggttct tccaaagaaa gatagtcctt
     5461 aagaagtgta ttgaaatcat gcacaaccat ttcctcaaag gagccttctc cttcaagtat
     5521 caaaaatccc atgggggcgt ttacaagtag cttgtggtat atgctgttca tatggcatct
     5581 ctttcccttc ctaccctttt tgtctatata cccctaaagg gaacaagtat tacagtaaca
     5641 tctcaaatat atccaatggt cccttggctc cattcaacac tccatttcac attattttca
     5701 cctgagtgta acaattttgc aatgaattgg ctaatatttt atggtatact aacatagata
     5761 cttaaaggga ggagacttaa aatgaattta tcaaaaggaa aaaacagcag gaaaataata
     5821 ttgctgatgc ttgtacttgc gcttggggtg tttgcaggtg cctgtgaaaa ggcagaagac
     5881 ccaagtgatg ttgaagtggt tgcaaaaatc aacgacgtgg agatcaccaa ggacgagttc
     5941 tacaactacc tgcttcagca gaatggacct gaagttcttg aggcattgat tctggaaaag
     6001 atggttatga tggaggttga ggcaaataat gttgtaatct ccgaggaagc catccaggaa
     6061 gagcttgatg tcatgaaaag ctcctatggt ggagatgaag cctttgaaga ggcccttgtc
     6121 tactacggat tcactgaaga gtcagttaag aagaacattc agcttaacct tggaatcgag
     6181 gccctgatgg agccatatat cgatataact gaggatgaga tccttcagta tttcattgca
     6241 aacaaagcgg agttcgatac ttcagaccag gtaaatgcca gccatatact tgtggataca
     6301 gaggaagagg caagggacat caaacaacag ctggatgacg gtggagattt tgcagagctt
     6361 gcagcccaat actcaaagga tgcttcaaat gcacagcaag gcggagcctt gggcttcttt
     6421 gggaagggac gtatggtcgc cccatttgag gcagcagcct tcagtatgga gccaggggaa
     6481 ataagtgaac cggttcagac tgactttggt ttccacataa tcaaggttga ggataggagt
     6541 gaaggacaag aggcacagct tgaagatgtt tccgaggata tcagggaaat cctcaaggac
     6601 aataaaatga actcagcata ctctgtgtgg tatgaggatg ttcaggagaa gtatgatgtt
     6661 gagaacttta ttctaaataa ctaatgaaaa aatctggatg ctgagcatcc agattttttt
     6721 atgaatatct acttgttttt atttctctcc aggaatcctg caaggtcaag ctccattgtg
     6781 aacctatcct ctcccttgcc gtactcattc tttgaataca agatcgactt gaatccaatt
     6841 ttatccctgt agagtcttat ggcaggctca ttttccacat caactgtaag gctcatctta
     6901 tcaaacccct ggtctagaag gtcctctgct attatcttca ggaactcaaa tccaaggccc
     6961 atgctctggt aatcctcgga aattgcatag tcaaaaaggt aggccttgta gaagtcctcc
     7021 caatccctca taagtattgc cagaccaaca acctggtgtt tgccttcctc ggttagtata
     7081 tatacgtttc catgtctgat ctgtggaacc aatccccatt gatccattcc aaggtcacca
     7141 aatatgtcaa gtccaaattc aaccatgttt ctcaatagct taagattgaa gtcctgtacc
     7201 agataaacct ttaaattgtc cacaccttca atatttttta ccaccttgtg gggtagccct
     7261 ttaacttctg ctaattgcat aattcatctc cttttctttt tttatgtaaa ttttataccc
     7321 aaaagcctag acctttaaag acttttttca gtgaaaggtc atttgtaaga tattggccta
     7381 tcgctatatt gcacaaaagc tgccaaaagt atataattac aaaagaaacc cataaggaga
     7441 gagataatgg aattcaagga taaaataact agaatgatgc agatactaaa aatcacaaac
     7501 tcccaattgg ccgagagact ggatgttgac atgtcacttg tcagtcggtg gagaacagga
     7561 aacaggctcc cctcccaaag gggtgaccat atggaacaga ttgcagatta cctgatggaa
     7621 tgtgcctttc aaaaggattt gttggatgag ttcacagagc tcttgggtct ttctgcagat
     7681 gcacacagaa atgaccacgc ttatctaagg tcccacctgc tcaactggct gcaggatgaa
     7741 aagtttcagg ataattctcc ctatatcagg aactttatcg acaaggttga gttttttacc
     7801 gaaaactcca acaatactag ccatgagaag ttgcatagtg ttcccaaata ttcagcaaag
     7861 gacatttacc ttggtacaga gggtaaacgt gaggcaatga taagattcct tcaccagatt
     7921 atcctatccg atgagattct tgacatatac ttctatagtg atgaatccca ggagtggatc
     7981 agtgatgata tagatttcta ctttaccttt aacggcatgc tgtcagatat tttggccaag
     8041 ggacacagat tgaagatcat ccaaccttat tacagggact tcaggtctat gatggaatcc
     8101 attgaaaggt cgcttccaat atatatttca ggccagattc agacctacta ctatccccag
     8161 gagatgaaga gtatttttgg aaatacagtg gccttggctg gagacagggc tgccatcaaa
     8221 tcagcgtcca taaagtcaaa ctcccataac agcataaact tctatgtgga taaaaaggat
     8281 gttgtcctct caatgaagga caatctattg agctttatcg gggaatgtga ttcccttatg
     8341 agtatatttg gctttcataa tcttaaggag ctaattgcct acctcctgga ccagggggac
     8401 agtccagggg atctgatagc cctccaccag accttgagca caatgacaat accaatggaa
     8461 tttctttata tgctctcaga ttccaaggac cttcataagg cccatattaa gaggaccgat
     8521 ctacttaaag acaatctaaa aaaatacaag tacattgaga tattctccct ccccagcctg
     8581 gatgaactgg atgctggaaa agagcaggtt gagctgtcaa atcacctgtc ctcagaccct
     8641 atctactatg atagaaacac attcaggatc cacctggaga atgtaataga ctggctggag
     8701 acctacccca actacaatgt aataatactt gacaagctct ttctcaagga tgtacggatc
     8761 acggtcaaaa aggacaggac agccattctg tacaaaatga ggccttcacc aatagcaata
     8821 gcctttgaca ataaggaaat cattgaagcc ttctactcct atattgtctc tgttatcaat
     8881 gaatccaacg aaaagacctt ggacaggaga ctttccattg aaagactcaa ggattatatg
     8941 agtaagcttt aggaagcaaa gacctgcaag gcatttgggg tttaaactaa cccatttgcc
     9001 acttgcaggt ctttctataa atgagcatca cttgacatgc tatacccccg gggtgtataa
     9061 ttgcttttag aagaaactta tggaggtgca ttagaaaatg gaagtcaatc taaatttagg
     9121 aagcaatcca ggaaaggaca agccaaaaat gaacccggag acaacctatg accttattgt
     9181 tctgggagga ggtcccgcag gtcttaatac ggcactttat gccaagagaa agggtctcca
     9241 ggtaggagtt atcgctaggg atatcggtgg acaggttatg gacacatcct cagttgagaa
     9301 ctatttaggc ttcacttccc tttcgggaga agagcttatg aagaagtttg ttggtcatgt
     9361 aaataagctg gaggtacctg ttctggagta tgaggagctg gaatcagtca atgtaaaaga
     9421 caattcaaat atcagggaag tggtcctgaa aaatggccaa aggttcaata cttattcagt
     9481 tgtcatcgca actggaagta agcctagaaa gcttggagta cctggagaaa aggaatattc
     9541 cggcaagggt gtttgctact gtgccatatg tgatggtccc ctttttgcag gtgaagaagt
     9601 aattgtggct ggcggtggaa actcagctgt tgaggccgca atagacctgg ccaagattgc
     9661 caagaaggtt acaatagtcc acagatctca gttcagggca gaccaaatac tgatcgatca
     9721 gctttcaagg ctggataatg ttgaagtcaa gcttcagacc cagatcctgg aagtggaagg
     9781 ggacaagttc atgaccggtg taagggttgc ggacagggaa tcaggcgagg aatacattgt
     9841 ggagggcgca ggtctatttg ttgaaatagg ctatcttcca aacagcgaaa tgttctccaa
     9901 tctgttgagc ctaaatgaaa gaaatgaaat tgtagttgat aggtatggca aaaccagcgt
     9961 agatggaatc tatgcagccg gtgatgtc
//
LOCUS       DASRQF010000213         3407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1130518, whole genome shotgun sequence.
ACCESSION   DASRQF010000213 DASRQF010000000
VERSION     DASRQF010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130518"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..585)
                     /gene="ispF"
                     /locus_tag="VFD79_05655"
     CDS             complement(100..585)
                     /gene="ispF"
                     /locus_tag="VFD79_05655"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002835155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HZH93570.1"
                     /translation="MRVGIGYDVHKLVEGRKLIIGGVQIPHEMGLLGHSDADVLVHSL
                     MDAILGAMSAGDIGEHFPDTDMEYKDISSMVLLERVFQVMREKGYRLVNADNVIAAQR
                     PKLAPYIDQMRENIARVLQVPVEDIGVKATTTERLGFEGREEGISSYSTCLLEKSIDN
                     G"
     gene            complement(599..1321)
                     /gene="ispD"
                     /locus_tag="VFD79_05660"
     CDS             complement(599..1321)
                     /gene="ispD"
                     /locus_tag="VFD79_05660"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HZH93571.1"
                     /translation="MYKGNFVSVIIAAAGMSNRMGSKINKQFIVIDNKPILAHTIEKF
                     EACKYVDEIIVVSKEQEVEYCRKEIVRKYGYRKVTNIIKGGKERQDSIYNGIMALNER
                     TDIVLTHDGARPFVRMESIVNGIKGALDYGSCVIGVPLKDTIKVIEEDNQVHHTPNRA
                     LLWAAQTPQCFQIDLLKEGYEYAISEGILGTDDSSLVEKKGYPVKMIMGNYDNIKITT
                     PEDLVVAQSIAKDKTLSISGRD"
     gene            complement(1362..2468)
                     /locus_tag="VFD79_05665"
     CDS             complement(1362..2468)
                     /locus_tag="VFD79_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HZH93572.1"
                     /translation="MVKKIARVLIGLFGMLLGYLAYTGFKGLELVNVTGMAELVVILI
                     FISIFGIIFFIFSSKIIKRGQNIAKVIEGELQRFSIQNIALGAIGLIIGLVIAYLLSQ
                     PLYRLEIPYIGVVASIILYGLFGYLGVTIPNRNIEDITTTLGNFTGNIGTGKKNHKER
                     GAEKGACPKILDTSVIIDGRIADIVKTGFIEGPLVIPVFVLEELQHIADSSDGLKRNR
                     GRRGLDVLNRIQNELDTEIIIHEKEFDEVSEVDSKLLKLTQYMKGKIITNDYNLNKVA
                     EVHRIEVLNINELANAVKPVVLPGEEMVVQVVKDGKEAGQGLAYLDDGTMIVVESGRK
                     YIGETIDVIVTSVLQTSAGRMIFAKPKSLYDKAV"
     gene            complement(2536..3054)
                     /locus_tag="VFD79_05670"
     CDS             complement(2536..3054)
                     /locus_tag="VFD79_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HZH93573.1"
                     /translation="MFKIGDKIVYPMHGAGVIEKLETKEILGEKREYFIIKMPIGDMK
                     VMVPVSNVDEVGVREIISPDEIEDIMSILRGHKTKMPQNWNRRYRINMDRIKTGDIHE
                     IASVVRNLTLLDNEKSLSTGERKMLSNAKQMLLSELVLVTGNSYEEVEKLVDKEIMEN
                     EGIEEEGDDFEE"
BASE COUNT          896 a          873 c          597 g         1041 t
ORIGIN      
        1 cagagggacg gaaatcttcc agccagggat ctcctctctt ggaccatgat acaatactag
       61 ctcccatcga agctgacaca aataagttta cacaaaagcc tatccattgt caatggattt
      121 ttccagcaaa catgtgctgt aggaggatat tccctcctct cgaccttcga agccgagtct
      181 ttcagttgta gtagccttca ctcctatgtc ctccacagga acctggagga ccctggctat
      241 gttctctctc atctggtcga tgtatggagc aagttttggc ctttgtgcag cgattacatt
      301 gtctgcattg accagcctgt agcccttctc cctcatcact tggaataccc tctcaagaag
      361 aaccatgctg gatatgtcct tgtattccat atccgtatca ggaaagtgct cgccaatatc
      421 tccggcactc attgccccaa gtatggcatc cataaggctg tgaaccagaa catccgcatc
      481 ggaatgacct aaaagtccca tttcatgggg gatctggacc cctccgatta ttagcttcct
      541 gccctccacc aacttgtgga catcatatcc tattccaact ctcatcaagc ttcacatcct
      601 agtctcttcc cgagatgctc agggtcttgt cctttgctat ggactgggcc acaacaagat
      661 cttcgggagt agtgatttta atattgtcat agtttcccat tatcatcttc acaggataac
      721 ctttcttctc caccagggag ctgtcatccg ttcccagaat cccttcggaa atggcgtatt
      781 cataaccttc cttcaggagg tcgatctgga agcactgggg agtctgagcc gcccaaagga
      841 gtgctctgtt tggagtgtgg tggacctggt tgtcctcctc gattaccttg atcgtatcct
      901 taaggggcac tccgattaca catgatccat agtccaaggc accctttatt ccatttacta
      961 tggattccat ccttacaaag ggtcttgctc catcatgggt caggacaata tctgtccttt
     1021 cattcagggc cattataccg ttgtaaatgg aatcctgcct ttccttgcca cccttgatta
     1081 tgtttgttac ctttctatag ccgtactttc tgacaatctc cttgcggcag tactctacct
     1141 cctgctcttt tgacacgaca attatctcat ccacatactt acatgcttcg aatttctcga
     1201 tcgtgtgggc aagtatcggc ttattgtcaa tgacaataaa ttgcttgttt attttgctgc
     1261 ccatccgatt gctcatgccg gcagcagcta ttatcactga cacgaaattt cccttataca
     1321 ttagaccacc tcaaaaatgt ttattgctta tcaactaacc cctacaccgc cttgtcatac
     1381 aatgactttg gcttggcaaa tatcattctg cctgctgagg tctgaagaac agatgtaact
     1441 atgacatcaa ttgtttctcc aatatatttt cttcctgatt ccactactat catggttcca
     1501 tcgtcaagat atgcaaggcc ctgtcccgct tccttaccat ccttgacaac ctggacaacc
     1561 atctcttccc ctggaagaac aaccggcttg accgcatttg ccaactcgtt tatgttcagg
     1621 acctcgattc tgtgaacctc tgcgaccttg ttgagattgt agtcgttggt aatgatttta
     1681 cccttcatat actgggtaag cttcaaaagc tttgagtcaa cctcacttac ctcatcaaat
     1741 tccttttcat ggatgattat ttctgtgtcc aattcattct ggatcctgtt gagaacatcc
     1801 agaccacgcc ttcccctgtt cctcttaaga ccatctgagg aatctgcaat atgctgaagc
     1861 tcctcaagaa caaagaccgg tattacaagc ggaccctcta taaaacctgt cttcacaata
     1921 tctgcaatcc taccgtctat tattacactt gtgtcaagaa tcttggggca agcccccttc
     1981 tcagcacctc tctccttatg gtttttcttc cccgttccga tgttgcctgt gaagttgccc
     2041 agtgttgtgg ttatatcctc gatgtttcta ttgggtattg taaccccaag gtatccgaac
     2101 aaaccgtata ggattattga tgcgacaacc ccaatatagg gaatctccag tctgtaaagg
     2161 ggttgactca acagataggc aatcacaagt cctattataa gtccaatcgc tcccagtgca
     2221 atattctgga tggagaacct ttgcagctca ccttcaatga cctttgcaat gttctggcct
     2281 ctcttgatga tcttagaaga aaaaataaaa aatattattc caaatataga tataaaaatt
     2341 aagattacaa caagctctgc cattccagtg acattgacca actcaaggcc cttgaaccca
     2401 gtgtatgcca aatagcccaa aagcatacca aagagtccga taagaaccct ggcaattttc
     2461 tttaccataa ttccacctcc attttttcca tagtttccat tataaaccta ttggcagggc
     2521 aataccagta taagtttact cctcaaagtc gtctccttct tcctcgatgc cttcattctc
     2581 cattatttcc ttgtcaacaa gcttctcaac ctcttcgtag gagttccctg taacaaggac
     2641 cagttcagac aggagcatct gctttgcatt gctaagcatt tttctctcac ctgtggacag
     2701 gcttttttca ttgtcaagca aggtaaggtt tcttactaca gatgcaattt cgtgtatgtc
     2761 ccccgtcttg atcctatcca tgtttattct gtatcttcgg ttccagttct ggggcatctt
     2821 tgtcttgtga cccctaagaa ttgacatgat gtcttctatt tcatcagggg aaatgatttc
     2881 ccttacacct acctcatcaa cgttagatac cggaaccata accttcatgt caccaatggg
     2941 catctttatg atgaagtact cccttttctc accaagaatt tccttggttt caagcttttc
     3001 aataactccc gcgccatgca taggataaac aatcttatcg ccaattttga acatatcaaa
     3061 acctccccat agtattccct cattatacta ttataaccca gatacgccca tatgtaaagt
     3121 taatatatta ccacataagt gtaaatcatg tcaacatgat tttgagttaa tgaccagaac
     3181 ttaacatgtg aaaaatttga ccctcatttc ggtttgacaa cctaatgcag taaaccttat
     3241 aattgaattg agaacttaac aagaggaggt tcatatggtt aacgggacaa atcaggaaaa
     3301 tctaatagaa gaactacaaa atcaagtatc tgaaacactt gtaagacaca agagcatttt
     3361 ggacataatg acgaagttgg atgagtacaa tgcccggatc aacaggg
//
LOCUS       DASRQF010000214         3311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1758498, whole genome shotgun sequence.
ACCESSION   DASRQF010000214 DASRQF010000000
VERSION     DASRQF010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1758498"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFD79_05675"
     CDS             <1..201
                     /locus_tag="VFD79_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HZH93574.1"
                     /translation="QPYHHGDLEILEKRIAGIYSEDRAKAIRKSHENPMIKQIYEEYL
                     GEPYGEKAHELLHTYYVPRIKM"
     gene            complement(245..1555)
                     /locus_tag="VFD79_05680"
     CDS             complement(245..1555)
                     /locus_tag="VFD79_05680"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HZH93575.1"
                     /translation="MVQKTVIYFFLMAMALSPVISGTDVTPNKIVNPIESTVNVGDEN
                     LPPPVGDYLENPLLRLQEGTGYSSKDYSEIARGLSSRYPEILTYKTIGYSRDGKEIYS
                     IVMTENVKTVMNRDDFNTFRMHYLIDAGVHGRETVNPAIVLKQIEDYAADYYKDSQIE
                     GFNLRDILSKSVFHFVPLVNPDGHDLSKFGPDSVLTSSLKDALANIDDKDYSQWKANI
                     AGVDLNKNFPDFHLQDGSDGLTDKWQVHPGNFLSSRPAGEYYPGVSPGSEPETKALME
                     YMKSFDFRSYVSYHSRGNVVYYEYKWYPQSYRDKALILAQATNEVTGYRLIPDTGEGS
                     GYSTSYHAALSLKPSLTIETLPHNTALPSGNRTYLDAYEKTWLLPLHYQRIGEKTGYF
                     TYRLYVDGKYVRDYPDEAYARAIAGSLGGEIIQGEGVPEQIISK"
     gene            1735..2157
                     /locus_tag="VFD79_05685"
     CDS             1735..2157
                     /locus_tag="VFD79_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZH93576.1"
                     /translation="MKKILVTTDGSKYSQKALFKARQMASDMGCEVTILSVVNLWDNV
                     YSHNMDIKEQLGKTEMDRTQEILREAEEIFKGFEGEWKTVYKVGDVVDVIVSFAEEGN
                     YDLVIMGSRGLGAFSRTLLGSVSDKVIHHIKTSVMIVK"
     gene            2307..2702
                     /locus_tag="VFD79_05690"
     CDS             2307..2702
                     /locus_tag="VFD79_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HZH93577.1"
                     /translation="MIVEHLLEMIVPYITASLELIGVAIITIAAVRGVFQFFENGFNF
                     RGDDVAIDFAKAMTLSLEFKLAAEIIKTVVIRTIDEFIILASVAILRVVLSFVLHWEL
                     KSGAKNVDTYCEDPDELATDPSQNVKQIR"
     gene            2825..>3311
                     /locus_tag="VFD79_05695"
     CDS             2825..>3311
                     /locus_tag="VFD79_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HZH93578.1"
                     /translation="MKKLLTGNEAIARGAYEAGCHVATAYPGTPSTEILENVSTYQEI
                     YSEWSTNEKVAMEVAAGASIAGARSMVAMKHVGLNVAADPLYTIAYEGVNGGLVVITA
                     DEPGMHSSQNEQDNRLTAVHAKVALMEPSDSQECKDYIKKAFEISEEYDTPVLFKVTT
                     RI"
BASE COUNT          981 a          646 c          779 g          905 t
ORIGIN      
        1 cagccctacc accacggaga ccttgaaatc cttgaaaaaa gaattgctgg aatctattcc
       61 gaggacaggg ctaaggcaat cagaaagtcc catgagaatc caatgatcaa gcaaatctac
      121 gaggaatacc ttggagagcc atacggagaa aaggctcacg agctgctgca cacatactat
      181 gtgcccagga taaagatgta gagacaaaat aagttgctgc cattcatcag ttggcagcaa
      241 ctttctattt gcttataatt tgttcaggaa ctccctctcc ctgtataatt tctccaccaa
      301 ggctcccagc aattgccctt gcataagcct catcagggta atccctaaca tatttgccat
      361 ctacataaag tctgtaggtg aaatagcctg ttttctcacc tatcctctgg taatggagtg
      421 gaagaagcca ggttttttca taggcatcaa ggtaggtcct gttacctgaa ggcaaagctg
      481 tattgtgggg tagggtttca attgtcagtg atggcttaag ggataatgcc gcatgatatg
      541 atgtggaata gccacttcct tccccagtat ctggaataag cctgtagccg gtcacttcat
      601 ttgtagcctg tgcaaggatc aaggccttgt ccctgtagga ttgaggatac catttatatt
      661 catagtagac aacgtttcct cttgagtggt aggaaacata gcttctaaaa tcaaagcttt
      721 tcatatactc cattagcgcc tttgtctcag gctcgcttcc aggagatact cctggatagt
      781 actccccggc tggtctgctt gacaagaagt ttccagggtg gacctgccat ttgtcagtca
      841 atccatctga tccatcctgg aggtggaaat ccgggaagtt cttgtttagg tcaactccgg
      901 cgatgtttgc cttccactgg gaatagtcct tgtcatcgat attggccagg gcatccttta
      961 aggagcttgt taagactgaa tcaggtccga actttgacag gtcatgacca tctggattta
     1021 ctaggggaac aaaatgaaat acagattttg acagtatatc cctaaggtta aagccctcaa
     1081 tttgggagtc cttgtagtaa tcggcagcgt agtcctcaat ttgctttagg actattgcag
     1141 ggttgacggt ttctcgtcca tggacacctg catcaataag atagtgcatc ctgaaggtgt
     1201 taaaatcatc cctgttcata actgtcttaa cattctccgt catgacaatg gaatagattt
     1261 ccttgccatc acgggaatag ccgattgtct tgtaggttag tatctctgga tagcgggaag
     1321 agagccccct ggcaatctca gagtaatcct tggacgaata ccctgtcccc tcttggagtc
     1381 ttagcaaggg gttttccagg tagtcaccaa ctggtggagg cagattttca tctccaacat
     1441 taactgtgga ctcaattgga tttactattt tgtttggtgt cacatctgtg cctgaaatca
     1501 caggggaaag tgccattgcc atgagaaaaa agtatatgac tgttttttgt accatagctc
     1561 cacctcccta aaagtaaatc aaagattaaa gttttgtatc atatacccag aatcagggtg
     1621 atagaatccc taatatatcc taaagcgtta aagtggcagg ctataagggt aaatatagta
     1681 acaaggatat ggataaggat agctttggaa accaagatta agggagggaa gaacatgaaa
     1741 aagattcttg taaccactga tggctcaaaa tactctcaga aagcactttt taaagccagg
     1801 caaatggcaa gtgacatggg ttgcgaagta acaatcctat cagttgtcaa cctgtgggac
     1861 aacgtgtatt cccataatat ggacataaag gagcagttgg gcaagactga aatggatagg
     1921 acacaggaaa ttctcaggga agcagaggag atattcaagg gctttgaggg agagtggaag
     1981 actgtataca aggtaggtga tgttgtggat gtcattgtaa gtttcgctga agaggggaac
     2041 tatgatcttg taattatggg cagcagaggc cttggtgcct tttcaaggac tcttctggga
     2101 agcgtttcag acaaggttat ccatcatatc aagacatcgg tcatgattgt aaaataacag
     2161 atattgccaa gcctctcagc aaagagaggc tttttgttga actgatgcaa caaatctgaa
     2221 aaaaatattt caagttgcct gtttattgtg atacaataat gggcagggga attattctca
     2281 aatattttta ttaaggagcg gtaaaagtga tagttgagca tttattggaa atgatagtac
     2341 cctacatcac agcttcactg gagttgatag gtgtagcaat tattacaata gcagcggttc
     2401 ggggtgtatt ccagttcttt gagaatggat tcaacttccg cggagacgac gttgcaatcg
     2461 actttgcgaa agcaatgaca ctaagccttg agtttaagtt ggcggcggag attatcaaga
     2521 ctgttgtcat aaggacgata gacgagttta taattctagc atccgttgca atactaaggg
     2581 tagtgctttc attcgtactt cactgggagc ttaagagcgg tgccaagaat gttgacactt
     2641 actgtgaaga cccagacgag ctggcaacag atccaagcca gaacgtaaaa cagatcagat
     2701 agataattat aggagtaata tattggttta aagcaatcga tatattactt ttttgtaatt
     2761 attgtccatt ttcagaatta tcctatataa tataagtaag aaatctaaat acaggagtga
     2821 ttaaatgaaa aagctattaa ccggaaacga agcaattgca agaggagctt acgaagccgg
     2881 ctgtcatgta gccacagcat atccagggac accttcgact gagatcctgg aaaacgtttc
     2941 cacgtatcag gaaatctatt cggaatggtc cacaaatgag aaggttgcaa tggaagtagc
     3001 tgcaggtgca tcaattgcgg gagcaagatc catggttgcc atgaagcacg ttggacttaa
     3061 cgttgcggca gacccccttt ataccatagc atatgaggga gttaacggcg gtcttgttgt
     3121 tataacagct gatgagccgg gaatgcacag ctcgcagaat gagcaggaca acagactgac
     3181 tgcggttcat gccaaggtgg ccttgatgga gccttcagac agtcaggaat gtaaggatta
     3241 tatcaagaag gcctttgaga tttctgagga atacgacaca cctgttcttt tcaaggttac
     3301 cacaaggatc t
//
LOCUS       DASRQF010000215         4916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1405058, whole genome shotgun sequence.
ACCESSION   DASRQF010000215 DASRQF010000000
VERSION     DASRQF010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405058"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFD79_05700"
     CDS             <1..193
                     /locus_tag="VFD79_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93579.1"
                     /translation="TFTRVFMPLYFLFNTILILASVYIEISIQGLEMCDLVIRHRGKS
                     DTEKYGKRLKLRVIKKEKL"
     gene            291..1526
                     /locus_tag="VFD79_05705"
     CDS             291..1526
                     /locus_tag="VFD79_05705"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HZH93580.1"
                     /translation="MRYLYKEYYNLKTIDERKSKANQRRRYDSTINFDLEIKPINQPE
                     KFSLYYVPTNSTLKLAAEIARLDVILEMNYSKLPGLAQHNFFIDMLSRELFSTNELEG
                     VKSSKEEIVRTTRSILGDSKKKPARFKNVIKSYLELKEGNLKPPVDAKDCRKIFDEIT
                     SDGISKDDYPDGKFFRREATYIYRNDKEIHRGITSVDGTEELIIEKINSLFRFMNDSS
                     LDLYDLIKIAVSHYYFGYIHPYYDGNGRTGRFISSIFLKDKFSWLTAMSLSQGCNKER
                     ERYLKIFDTTNQISSQGEMNFFVDEFLQIIESGQKDILEILVQKSDLLNIIIDKINKD
                     ESLANEDEKIIMSIMAQEFHFNPSKEGIGVTELKPVFGYAEDTIRIKLKKLYERGLVE
                     KIKSRPVKYVISSKYLENS"
     gene            1619..2506
                     /locus_tag="VFD79_05710"
     CDS             1619..2506
                     /locus_tag="VFD79_05710"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZH93581.1"
                     /translation="MKQKETINSMMVMLGAVLWGTVGTAQHFAPEGATPMSIGAARLA
                     LGGGVLLIYALLKGKLTGREIFSRKSTYIGAVSMASFQLTFFAGVLLTGVAMGTVVAI
                     GSAPIFAGLISLISSKENPGLKWLISTLVSVLGCVLLVVGGDELSVNTTGIILALGAG
                     LSYAIYTASSKNLLVDFTPEAVTGIVFSMGAIIMSPFLLIYSLDWLFTARGIIVTMHL
                     GLITTALAYFVFTYGLSGIKLHKAVTLTLAEPVTAALLGVFLLKENLSMISGVGMGLV
                     FAGLVILSISRPKRRYPQT"
     gene            2720..3601
                     /locus_tag="VFD79_05715"
     CDS             2720..3601
                     /locus_tag="VFD79_05715"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZH93582.1"
                     /translation="MNNSEFKYSLMALLGAILWGTIGTAQHFAPAGASPLSVGAVRLA
                     VGGVAIMLYAFLRGKLKGREMLSKVGTYIGAASMASAQITFFVAVKMTGVAMGTVVAI
                     GSAPIFAGLISTIINKKIPGIKWMVSTMVSVIGCVLLVIGGEALTIEMNGIILALGSG
                     FFYAVYTAATKNLLVKYSPEIVTGIIFTIGALLLSPFLLIYSLDWLKTPQGIGVALHL
                     GLVTSALAYIIFSYGLSGIKLHKAATLNLAEPVTAALLGVFFLKESLSLLSGVGMGLV
                     FAGLVILSISRPAKKIA"
     gene            complement(3660..>4916)
                     /locus_tag="VFD79_05720"
     CDS             complement(3660..>4916)
                     /locus_tag="VFD79_05720"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HZH93583.1"
                     /translation="EGYAAKILYDNLQPGMNPMDERNLLVFTTGPLTGTRDPSSGRLV
                     IGYKSPLTGTIGRSSAHQELALTTFDSAMDKIGHKAYHDTLNVKAWACFGCPIACGRH
                     TEIMEGKYKGESGGGPEYETVGAFGSKCYVDDLNAITEAGYICNRMGLDTISTGQTIA
                     SAMEWFEKGLIDLETTGGIDLSFGNAEAMVKMTEMIAKREGYGDVLAEGSLRAARKIG
                     PEAEKYVFQVKGMELASCGVRASKGESLSHLISPRGADHLRPYASVIDAFGYLEPELG
                     INDKVSPFEDENKWWVKPFMELSMMTNLLGVCLFASITLAVKGSTWTGLYNAATGDEK
                     SLQEMMEAAERVINLERLFNAREGFGRKDEKLPERLSKEPAPDGLGKGQVANAEVMLD
                     EFYESMGWDKETGHPKDETLMRLKLK"
BASE COUNT         1434 a          948 c         1100 g         1434 t
ORIGIN      
        1 aacatttaca agggttttta tgcccttgta ttttttgttt aacacaattt taatacttgc
       61 atctgtttac attgaaatta gtatccaggg acttgaaatg tgcgacctgg tgataaggca
      121 taggggcaag tcggacacag aaaaatatgg gaaaaggtta aaattacgcg taattaaaaa
      181 agaaaagctc taattacaga ggaaaaacaa aaaaagctgg ttaattacca tcaataagtg
      241 tataattata ataattatta atgaaattat gtggatagag aggttttgat atgcgttatc
      301 tatataagga atactataat ttaaaaacga ttgatgaacg taaatctaaa gctaatcagc
      361 gaagaagata tgatagcacc ataaattttg atctggaaat taaacctatc aatcaaccgg
      421 agaagtttag tctttactat gtaccaacta actcaactct aaaactagca gcagaaattg
      481 ccagactgga tgtaattctt gaaatgaatt atagcaaact cccaggattg gcccaacata
      541 atttttttat agacatgcta agtagagagt tattcagtac caatgagctg gagggtgtta
      601 aaagttctaa agaagaaatt gtgaggacta ccaggagtat tcttggagac agtaagaaga
      661 agccggcgag atttaagaat gtcatcaagt cttatcttga gttaaaagaa ggtaatttaa
      721 agcctccagt tgacgctaag gattgtagaa agatatttga tgaaatcact tcagatggaa
      781 tttcaaaaga tgactatcca gatggaaagt tttttaggag agaagcaaca tatatataca
      841 ggaatgataa ggagattcat agaggaatta catctgtaga tggcacagaa gaattgataa
      901 ttgaaaaaat aaactcctta ttcaggttta tgaacgactc atctttagac ttatatgatt
      961 tgataaaaat tgcggtatca cattattatt ttggatatat tcacccatat tatgatggca
     1021 atggaagaac tggaagattc atcagtagca tattcttaaa ggataagttt tcatggctta
     1081 ctgcaatgtc gttgtctcaa ggatgtaata aagaaagaga acggtattta aaaatatttg
     1141 atacaactaa tcaaatctcg tctcagggag agatgaattt ttttgtggat gagtttttgc
     1201 agataattga atcaggtcaa aaggatattt tggaaatcct agtacagaaa agtgatctat
     1261 taaatattat tattgataaa atcaataagg atgagagcct agccaatgaa gatgaaaaaa
     1321 tcatcatgag tataatggct caagagttcc atttcaaccc atccaaagaa gggattggag
     1381 tcactgagtt gaaaccagta tttggttatg ctgaggatac cattaggata aaactcaaga
     1441 aactctatga aagaggttta gttgaaaaga tcaagagtag accggtgaag tatgttatat
     1501 cgagtaaata tcttgaaaat tcatagtaaa atatgaaacg taaaacaaca ttggagacgg
     1561 ttcttttttg ttggataaca ttaagattcg tctttttgta tagagggagt gatttgtcat
     1621 gaagcagaag gaaactataa attcaatgat ggttatgctt ggagctgtac tttggggaac
     1681 agttggaaca gcccagcact ttgctcctga aggagccacc ccaatgtcaa ttggtgctgc
     1741 caggcttgcc ctgggaggag gagtacttct gatctacgca ctcttgaagg gaaagctgac
     1801 gggaagggag atattctcaa gaaagagtac atatattgga gcggtcagca tggcatcctt
     1861 ccagcttaca ttctttgcag gtgtcctcct cacgggggtt gccatgggaa ctgtggtggc
     1921 aatcggcagt gcacccatat ttgctggact catatctctt atttccagta aggaaaatcc
     1981 tggactcaaa tggctgatat caacccttgt gtccgttttg ggctgtgttc ttcttgtggt
     2041 tgggggagat gagctttctg taaatacaac aggaataata ttggcacttg gtgcaggctt
     2101 atcttatgca atctatactg cttcatcgaa gaacctcttg gtagacttca ctccagaggc
     2161 agtaactgga attgtatttt ccatgggagc aattatcatg tccccatttt tgttgatcta
     2221 tagccttgat tggctattta cagccagggg aattatcgtt accatgcacc tgggactgat
     2281 taccactgcc cttgcatatt ttgttttcac atatggccta tcagggataa aactacacaa
     2341 ggctgtaacc cttacattgg ctgagccggt tacggcagcc cttttgggtg tatttctcct
     2401 taaggaaaac ttgtcaatga tatcaggagt aggcatggga cttgtgtttg cgggcctggt
     2461 gattctttca ataagcaggc ctaaaaggag atatccacag acataaataa tatttcataa
     2521 tattaaaagg cagatcattt tcaagtgaga ataatggtgt gtctttttca ttgtcaaaat
     2581 gtctttgtct gaattgacag acaaatatta ttatgcttta attagtgtat taaagagaat
     2641 gggaagcatg accaggacga ttcattttgt cagcaagaaa aaaggttcgt ctctttaacc
     2701 aaagaggaga ggatatctat tgaacaatag tgagttcaaa tatagcttaa tggcattgct
     2761 aggtgcaatt ttatggggaa ccattggaac ggcacagcat tttgctcctg caggagcctc
     2821 gcctctatca gttggtgcag tcagactggc cgtaggcgga gtagcaataa tgctctatgc
     2881 attccttaga ggcaagctca agggacgaga gatgttgtca aaagtaggta catatattgg
     2941 agcagcaagc atggcctcgg ctcagattac attctttgta gctgttaaga tgaccggggt
     3001 ggcaatggga acagtggttg caatcggaag cgctccaata tttgcaggtc tgatttcaac
     3061 aatcataaac aagaagattc caggtatcaa gtggatggta tcaacaatgg tttcagtaat
     3121 aggatgcgtc cttttggtta taggtggcga agctctaaca attgaaatga atgggataat
     3181 cctggcgctg ggatcgggct ttttttacgc agtatacacc gcagccacaa agaatttgct
     3241 ggtaaagtat tctcctgaga ttgttacagg aattattttc acaattggag cattgctcct
     3301 ttctccattt ctgctgatct atagcttgga ttggctaaag accccacaag gaataggtgt
     3361 agccctacat ctgggattgg tgaccagtgc actggcatat attatttttt cgtatggttt
     3421 atcagggata aaactgcaca aggcagcaac cttgaatctg gctgagcctg ttactgctgc
     3481 ccttctggga gtgtttttcc ttaaggaaag cctgtcactc ctttcaggag tgggaatggg
     3541 gctggtattt gcagggctgg tgatattgtc aatctcaaga ccggcaaaga aaatagcata
     3601 agtaaaggga ctgtttggat gctacttaga agtatcatta tcaaacagtc ccttttgatt
     3661 tacttaagct tcagcctcat caaggtttca tccttgggat ggccagtctc cttgtcccaa
     3721 cccatgcttt catagaattc atcaagcatg acctcagcgt ttgcaacctg acccttgcca
     3781 agtccgtcag gtgcaggctc cttggaaagt ctctcaggaa gcttttcatc ctttctgcca
     3841 aagccctccc tggcattgaa caacctttca aggttaatga ccctttcagc tgcctccatc
     3901 atctcctgga gggatttttc atcacctgtg gctgcattgt aaagacctgt ccaggtgctt
     3961 cccttaactg caagggtgat gctggcaaag aggcataccc caaggagatt tgtcatcatt
     4021 gaaagctcca taaagggctt gacccaccat ttgttctcat cctcaaatgg agataccttg
     4081 tcattgatcc caagctccgg ctccagatat ccaaaggcat cgattactga tgcgtatggt
     4141 ctgagatggt ctgcgcccct tggagagata aggtgggaaa gggattctcc cttgcttgcc
     4201 ctcactccgc aggaggcaag ctccataccc ttgacctgga acacatactt ctcagcttcc
     4261 ggtccaattt ttcttgctgc ccttagggat ccttcggcca ggacgtctcc gtagccctcc
     4321 cttttcgcta tcatttcagt catctttacc atggcctcgg cattgccaaa ggacaggtcg
     4381 attccacctg tggtttccag atctatcaga cccttttcaa accactccat tgcagatgct
     4441 atggtctgcc cggttgaaat ggtatctaga cccatccggt tacagatgta gcctgcctct
     4501 gtgattgcat tcaaatcgtc cacatagcac ttggaaccaa aggccccgac tgtttcatac
     4561 tcaggaccgc cacctgattc gcccttgtat ttgccttcca ttatttcagt gtgtctgccg
     4621 caggctatgg ggcatccaaa gcaggcccat gcctttacat tcaaggtgtc atggtaggcc
     4681 ttgtggccga tcttatccat ggcagaatca aaggtggtca gcgccagttc ctggtgggca
     4741 gaagacctac ctatagtccc tgtaagggga gacttgtagc ctatgacaag ccttcctgat
     4801 gatggatccc gcgttccggt caagggacct gttgtgaata ccagtaaatt cctttcatcc
     4861 atcggattca ttccaggctg gaggttgtca tacaggatct tggcagcgta gccctc
//
LOCUS       DASRQF010000216         3980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_877170, whole genome shotgun sequence.
ACCESSION   DASRQF010000216 DASRQF010000000
VERSION     DASRQF010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877170"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            410..1846
                     /locus_tag="VFD79_05725"
                     /pseudo
     CDS             410..1846
                     /locus_tag="VFD79_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            1898..2443
                     /locus_tag="VFD79_05730"
     CDS             1898..2443
                     /locus_tag="VFD79_05730"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HZH93584.1"
                     /translation="MDEINIAAELYGGISHDRIQEVGLQWPCPDKDHPGTPILHVGEF
                     KRGKGLFTVNDFEYIGDQRSEEYPFILTTGRVTHQYHTGTMTRKVWTLALEYPEGFVE
                     IHTKDAEELGIKDHQPVRITSARGSIVAPAMITNRINEKTLFVPFHFAESPANRLTGQ
                     NLDPIINIPALKVSAVRIEVE"
     gene            2443..>3980
                     /locus_tag="VFD79_05735"
     CDS             2443..>3980
                     /locus_tag="VFD79_05735"
                     /inference="COORDINATES: protein motif:HMM:NF028488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZH93585.1"
                     /translation="METNRLYKINNDSLDQVLNIWNEDSSVYVPVGKGRDIQLMPLGQ
                     GQRTEDYINLTLPVKEMVFEQKEGLFKWTRNGEDMDVEGLAHQHSKNRILFGVRACDT
                     YGIAYTDRFYLGEFPDPNYKSRRDETIIIGVNCLRAGEHCFCTSVGTGVFSTVGHDLA
                     FTPFDSYYLVEVGTPKGQDLIDKAESHFTVTDESILEKKNDLLARVEESFPLKMDLTN
                     LWDDMAKTFNADFWLDEANACIGCTGCTNVCPTCTCFNVVEENTDENHGQRVRYWDSC
                     QIDHFTRNAEHHNPRDAISRVRYRIYDKLKYIEERFGYKGCSGCGRCTDVCPTYISII
                     DIIGEIQKAAKENPEPPAIHQITKIRHEIMDREIRSQNGLFSPDVATITRVKQETPEI
                     KRLFMKYDDPELHKNYEHKGQFFQVTVFGEGEVPLSIPYGPEMTDELVFDFKNVGSVT
                     EKLFQMKEGDKVGLRGPYGRAFPYESLKGRNLVFIGSGVAMAPLRTVIEPVMVNHEDF
                     KDVYV"
BASE COUNT         1235 a          792 c          941 g         1012 t
ORIGIN      
        1 ctttcccttt aacagcggcg gtatggcctt tgtctgtatg ggggtaggta ccgaataccc
       61 ttcagttttt aaagccttct gaattggctc aattaaattt aattctttaa atgacaatat
      121 aacttctcct ttgatatggc attaagactt gctatctgta ttaatacata tattatttga
      181 tttacctggc ttccaatttt tcactcaacg ctgaatcatt atataccata tccgggtttg
      241 gtgcaaattt ttagtccaaa cctacaaaag caaggttgta ctattgcatt tcttgttgca
      301 cataatggaa tggatacact aacttccatt gctattaatc atggcaatgt ggtaaaatca
      361 aagagtgaaa atttagaatt tatccattta aagagaagga gggattttgt tgaaacgtgt
      421 agaaaccact tgcggttatt gtggttgtgg ctgtggtatt tatgttaatg tggcagacga
      481 tgacacaatt ctaggtatat caccaagcta taatcacgta gtcagtaaag gaaagctttg
      541 ttcaaaaggg tggcacggtt ttgcatttgt aagagattca cgtcgactaa ccagtccctt
      601 gattcgtcag gatgatggta cctttagaga ggcatcatgg gatgaagcac tggataaggt
      661 tgcggaaggt tttaagaatg ccatgacgga tggggacaat accgaccgaa ttggagtcat
      721 ttcatcagca cgatgtacca atgaggaaaa tttcctgatg gcaaaaatgg cgagggccgg
      781 acttaggacc agcagtatcg accactgcgc ccgactctga cacagtccca cagtagaagg
      841 tctttctact tcattcggaa ctggaggcgc tacaaatacc atgaatgata tcagcgatgc
      901 tgagttgatt atggtcatag gatccaacac aacagagcag catccattgg caagcaccag
      961 gatattcgaa gccctggaca aaggcgcaga gctctatgta gttgacccaa gaaaaatacg
     1021 tctaacagat tttgctcatg gttacgcacc cattgagcca ggaacaaacc taccaatgat
     1081 caatgccatg atccatgtaa ttattgaaga agatttaata gatgaggagt tcataaaaaa
     1141 cagaaccgag aactacgaag aaatgaagga atccgtcaag gattgtacgc ctgaatgggc
     1201 tgaatccatt tccaaggttc ctgctgatac aattagacag tttgctcgta agtatgcaac
     1261 caccgataag gcaatgatcc ttttcaccat gggtgttaca cagcagacaa cagcagttaa
     1321 gagcatcagg gcattggcca acctggcaat gatcacaggc catgtgggaa gaccttcaac
     1381 aggaataaat cctttgcgtg gtcagaacaa tgtccaggga gctggtgaca tgggcgcatt
     1441 gccggatttc ctgccaggct accaacgacc tgaatggccc gaaacaaaag agcactacag
     1501 taaagtatgg ggagatttct catgcctaag aggtaacaat atcatacaga tgttcaacct
     1561 tatagatgag ggaatcatga aggctgctta cattgtcggt gaaaaccctg tcctttcgga
     1621 tcctgaccag acccatacaa tgcattgtct tgaaaaggta cccttcctgg tggtccagga
     1681 catattcctg actgaaaccg ctgagtatgc agacgttgta ttgccagcag caagcttttt
     1741 ggaaagggat ggaacctata caaacacaga tagaagagtg caaatgatac ataaggtaat
     1801 cgagccagtt gggaactctt ggccagactg gaaaatcatg gttgaaatca tgaaaaggat
     1861 cggaatggat gtggactatg aaggtccgtc tgatatcatg gacgaaatca acattgcagc
     1921 ggaattgtac gggggtatta gccatgaccg tatacaggaa gttgggcttc agtggccatg
     1981 tcctgataaa gaccatcctg gaacacctat tctacatgtt ggtgagttca agagaggcaa
     2041 agggctgttt acagttaatg actttgagta tattggagac cagagaagcg aggaatatcc
     2101 atttattctt accaccggac gggttaccca tcagtaccat actggtacca tgacccgaaa
     2161 ggtttggaca ttggctctgg agtatccgga aggatttgtg gaaatacaca caaaggacgc
     2221 tgaagagcta ggaataaagg accatcagcc tgtaaggata acctctgcaa gggggagcat
     2281 tgttgcaccg gcaatgatta caaatcgcat caatgaaaag acccttttcg ttcccttcca
     2341 ttttgcagaa tcaccagcca ataggcttac gggtcaaaat ctggatccaa ttataaatat
     2401 tccggcattg aaggtatctg ccgtaagaat cgaggtggaa taatggaaac aaacagactg
     2461 tataaaataa ataatgacag ccttgaccag gtgcttaaca tatggaacga ggacagctca
     2521 gtttatgtac cagttgggaa gggcagagat atccagctta tgcccctagg tcaaggccaa
     2581 cggacagagg actatatcaa tctgaccttg cctgtcaagg aaatggtttt cgagcaaaaa
     2641 gaaggactgt tcaaatggac cagaaatgga gaagatatgg atgttgaggg tcttgcccat
     2701 cagcattcaa aaaacaggat tctctttggt gttagggcat gtgatacata cggcatcgct
     2761 tatactgaca ggttctatct tggagagttt cctgatccaa attacaagtc cagaagggat
     2821 gagaccataa tcataggggt aaactgcctc agggctggtg aacattgctt ctgtacttca
     2881 gttggaacag gtgtcttttc aacggttggt catgacctgg ccttcactcc atttgattcc
     2941 tactatcttg ttgaagtggg aacacctaag ggtcaggacc taattgacaa ggcagaatct
     3001 cacttcacag taactgatga aagtatactt gagaagaaaa atgacctgct ggcacgggtt
     3061 gaggaaagct tcccactgaa gatggacctg acaaaccttt gggatgacat ggcaaaaacc
     3121 ttcaacgcag atttctggct ggatgaggca aatgcatgca taggttgtac tggatgcaca
     3181 aatgtctgtc caacctgtac ctgcttcaac gtagtcgagg aaaacacaga tgaaaaccac
     3241 ggccaaaggg taaggtactg ggattcctgt caaatcgacc atttcaccag gaatgctgag
     3301 caccacaatc ccagggatgc catttcaagg gtccgctaca ggatatatga caagttgaag
     3361 tacattgagg aaagattcgg gtacaagggt tgttcaggat gtggaagatg tacagatgta
     3421 tgtccaacct atataagcat aattgatatc attggagaaa tccaaaaggc tgcaaaagaa
     3481 aatccagaac caccagcaat acatcaaata accaaaattc gtcatgagat catggaccgt
     3541 gagataaggt cacaaaacgg ccttttctca ccagatgttg caacaataac aagggttaag
     3601 caggaaaccc ctgaaatcaa gcgtcttttc atgaaatatg acgatcctga gctacacaaa
     3661 aactatgagc acaagggtca gttcttccag gtaactgttt ttggagaagg tgaagttccc
     3721 ctatccatac catacgggcc tgaaatgact gatgagcttg tatttgactt taaaaacgtt
     3781 gggtcggtaa cagagaaact cttccaaatg aaggaagggg acaaggttgg cctcaggggt
     3841 ccatatggac gagctttccc atacgagtcg ttaaaaggca gaaatcttgt cttcattgga
     3901 tctggggttg ccatggcacc acttagaact gttattgagc ctgttatggt aaatcatgag
     3961 gatttcaagg atgtttatgt
//
LOCUS       DASRQF010000217        14310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_678829, whole genome shotgun sequence.
ACCESSION   DASRQF010000217 DASRQF010000000
VERSION     DASRQF010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 14310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14310
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678829"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..4262
                     /locus_tag="VFD79_05740"
     CDS             33..4262
                     /locus_tag="VFD79_05740"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF001688.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HZH93586.1"
                     /translation="MAENRTIKSLFSDDTQIKSFLEDLSIISVVLDNKSLKLSIKMED
                     RHWVTQKDKASIASVLEEKFKGLALDIDYTKIEANLNQDQLLDIIKRKISQNIPSSSS
                     WLEDVVLNEGDLINLVLPGDLPYRSVTRNGLLEELTEISKKSTGKNLNVDFMDTKEHE
                     GFLFDAEMEERKILKEMMLKKPAEKSEKKARNKDYSFGKRIKGDPIKIIDIDMQTGSA
                     IIEGEIFQVETKSIKDNRTLVIFYLSDGTNSISSKVFLNGDQTEEFTSNIKEGTFVLL
                     SGDVVYDTYAKEMTIMLKSIQAKEQEIIKDNAKDKRVELHLHTQMSSMDGVSHTRDLI
                     KRAIEWGHEAIAITDHGVVQAFPDAMEMSKDIKIIYGVEAYMINDKKQIVTGNLEGRT
                     LDDFVVFDLETTGLSPKNDRITEIGAVKIQNGEIIDSFSQLINPHIPIPDLITRLTGI
                     TDEMVKDKPSIGEVLPEFLEFIKDSVLVAHNASFDMGFIREAYESIEIHLDNPVMDTL
                     ELSRAMFPTMKSHKLNLVAKHLKVELKNHHRAVDDALATGNILLRILELLGDRDLKNL
                     EDINGLTGNADSTKGDMHHIILLAKNQSGLKDLYRLISESHLNYFHRKPRMPKSLIES
                     HRKDLIVGSACEAGELYRAIIRNKPDQEIRDIAKFYDYLEIQPLGNNEYMVREGIVKN
                     QDELKSINKMIYKLGKSLNKPVVATGDVHFLDPEDEVYRRILMSGQKFQDADNQPPLY
                     FRTTDQMLREFDYMGEEIAKEVVITNPNKINSQIDKCIPIPDGTFPPEIEGSDQELRE
                     ITISKAVDTYGSPLPDLVKKRLERELDSIIKNGYAVMYIIAQKLVWKSMEDGYLVGSR
                     GSVGSSFAATMSGITEVNPLPPHYVCPSCRNSEFFTDGSIASGYDLPDKRCPKCDEDY
                     IKEGHDIPFEVFLGFEGDKEPDIDLNFAGEYQPRAHKYTEELFGEGKVFRAGTIGTIA
                     DKTAYGFVRKYFEEREMNVSQAEINRLLRGCTGIKRTSGQHPGGVMIVPDYKDIHDFS
                     PIQYPADDKKSGVITTHFDYHAISGRILKLDILGHDVPSIIRMLEDITGIDPTTIPLD
                     DKDTMELFTSTKSIGVDPDSINCPVGTMGIPEFGTKFVRQMLIETKPTTFSELVRISG
                     LSHGTDVWINNAQELVKNKVAPLAKVISTREDIMLSLIKAGMDKKQSFFIMEKVRKGK
                     GLKPDEEEAMRSLGIPEWYIESCNKIKYMFPKAHAAAYVTMSFRIAYFKVHHPQAFYA
                     TYFTTKSTDFDADLILKGEAAVRQKLKELEQKGMGATTKEKNQITVLEVVLEMYTRGF
                     TFDRVDLYRSDSDRFLINEKGILPPLKALDGVGENAARKIAEEREIKVFMSIEDLVKR
                     GKATRPVIDALKGHGCLDSLPESNQISLFNI"
     gene            4374..4835
                     /gene="rimP"
                     /locus_tag="VFD79_05745"
     CDS             4374..4835
                     /gene="rimP"
                     /locus_tag="VFD79_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HZH93587.1"
                     /translation="MSKKGFMTSIENLCEDVTSELGYELVDVEYIKESGDYYLRVYIH
                     KPGGVNLDDCQIVSEKLSEKLDDSDMLQKAYYLEVSSPGLDRPLKTDRDLERNLGKEV
                     EVNLYKAVDGVKKFEGILKSYKGDSVELETVDKVVEIPRDTVSLIRLTIKF"
     gene            4850..6067
                     /gene="nusA"
                     /locus_tag="VFD79_05750"
     CDS             4850..6067
                     /gene="nusA"
                     /locus_tag="VFD79_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009431486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HZH93588.1"
                     /translation="MNVDFINALEEIEREKGISKDIIFDALESALISSYKKNFGSSQN
                     VIVEMDRQSGDVEVFATKDVVENDHIEDELLQMALEEAKEINPNYEVGDVVKIAITPK
                     DFGRIAAQTAKQVVIQRIKDAERDVIFDEFISRENEIITGQIQRIAKNNVYIDLGKTE
                     GILPPSEQIAGEEYGQGDRKKLLILEVKKTTKGPQIVLSRANTGLVKRLFELEVPEIA
                     EGIVEIYSVSREAGSRTKIAVFSKNPDVDALGSCVGFKGARVKAIVDELNGEKIDIVI
                     WSKEPAEFIGNSLSPSKVVKVVPNEKEKSAIVVVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSETQYKEFMEKKAAQEEQVAVDEDVEVDLLVDGSATYPDQTSDENSEEGLDE
                     ISSETSDESSDEE"
     gene            6082..6345
                     /locus_tag="VFD79_05755"
     CDS             6082..6345
                     /locus_tag="VFD79_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HZH93589.1"
                     /translation="MSARKIPLRKCIACNEMKPKKELVRVVKNKENEVSVDLTGKANG
                     RGAYICPTIECFEEAYRNKKFQRALQTEITEEVYSGLKKVIDK"
     gene            6379..8430
                     /gene="infB"
                     /locus_tag="VFD79_05760"
     CDS             6379..8430
                     /gene="infB"
                     /locus_tag="VFD79_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019034564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HZH93590.1"
                     /translation="MTKVRIYELAKELNMSSKALMEKIEGLELKINSHMSSIEDEEAQ
                     LIKELLTEDKTEGKAVIEDELEIFDEDEKPKSKKPKKKQHKIEETPIEKEEKIIEKKT
                     IEIESEVIVKDLAEMIGVNSSQIITKLIGLGVMVNQNQAIDSEIAAIVADDFNVEVKT
                     LEPEVELTEEDLLDYDDKPEDMVARPPVVTVMGHVDHGKTSLLDAIRHTNVTDKEAGG
                     ITQHIGAYAVNINNKKIVFLDTPGHEAFTSMRARGASVTDVAILVVAADDGVMPQTVE
                     AISHAKAAGVPIIVAINKIDKPDSNPDRVKQELADNGLVPEDWGGDTIIVPVSAIQRT
                     GIEELLEMILLVADMQELQANPDRRAVGTIIEAQLDKGKGPLATVLVQKGTLHVGDMV
                     VSGTSSGRIRAMLDEKGKNVKKATPSIPVMILGLSEVPNAGDLLFAVENEKAARNIAD
                     ARKTLIREAQMKQSSKVSLDDLFEKIKLGEIKDLNIIVKGDVRGSIEALSQSLNKLDT
                     EEVRVNLIHGGVGGITESDIMLASASNAIVIGFNVRPNLNAIEVAKKEDVDVRTYRII
                     YEAIEDIQAAVKGMHAPKIVEEILGRAQVRATFRLPNGNTIAGIYVVSGKITRNANIR
                     LLRDDVVIFDGSVSSLKRFKDDAREVLTGFEAGLGLENFNDIKEGDNLEAYVLKEVKR
                     D"
     gene            8442..8819
                     /gene="rbfA"
                     /locus_tag="VFD79_05765"
     CDS             8442..8819
                     /gene="rbfA"
                     /locus_tag="VFD79_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HZH93591.1"
                     /translation="MNEKRINRISEEVKKVVSDLIQNEIKDPRISGMPSVNKVSVTKD
                     LRFAKIYISVLGSEEDKESTIEGLQNAKGFVRKEIGNRINLRHVPEPIFLLDESIEQA
                     LYMSKLIEEVNRGSKKDSDENEE"
     gene            8803..9771
                     /locus_tag="VFD79_05770"
     CDS             8803..9771
                     /locus_tag="VFD79_05770"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HZH93592.1"
                     /translation="MKMKNNLEAFKELIRTSERIAVVSHMNPDGDNLGSLLAAGLSLE
                     KMGKGVLFVKADIIPEDYRFLPGVERLSPVDETIDMLDLLLVLDSSDPDRLGENRFLI
                     DKSQKVVNIDHHVSNSEFGDINIVKPEMSSTGEILFELLLQMDMPIDSEIASLIYVAM
                     STDTGRFTYQGVTGKTHETVARLYGYGIDAYAINNSLYQRRSLERTKLFIRAVSNIRL
                     YKDNRIGIVRVTKSMLDETGSSMEDTEGIVEFIRDTDSVEAACILKEIDDGVVKISLR
                     TKNIVDANKVCSVFHGGGHSRASGATVEDTIDNVESRLLDEINKYL"
     gene            9789..10679
                     /gene="truB"
                     /locus_tag="VFD79_05775"
     CDS             9789..10679
                     /gene="truB"
                     /locus_tag="VFD79_05775"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HZH93593.1"
                     /translation="MNGIINFFKPAGMTSHDAVAYFRRLLGTKKVGHTGTLDPMATGV
                     LPICVGKGTKVSEYLLGVDKEYIAELTLGTSSDTQDSTGSVINSSDKVVDNDQIENAF
                     HSFQGEIDQIPPMYSAKKIGGKKLYELARSGIEVERKPRRINIKEIRILNNQDNRKII
                     FYTKCSKGTYIRTICNDIGDLLGTYGHMSYLLRVGVGTFRVEDSYGMDTLNEMDKEGL
                     LSIIQPIDNAIQFMDGINLDSKFYKPLTNGVRIELDKNLKVDTDKLHRIYVSDEFIGI
                     GSILEENGRKTLKMEKVLIR"
     gene            10684..11610
                     /locus_tag="VFD79_05780"
     CDS             10684..11610
                     /locus_tag="VFD79_05780"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HZH93594.1"
                     /translation="MEIIRIDNTNETRFQTSLALGNFDGVHIGHQKLIRSMIETANES
                     GFQPSMMIFENHTKSFVFKNGPRLISSNDQKNRFLEKLGVKTLYTMVFDDEIMTLSPE
                     EFVTSILYEKLNCRAITVGPDYRFGYKAQGNVDQLISLSRELGIKTDIIQPVYHNDIL
                     VSSTRVREHLTDGEILEANTLLGRPFSVIGEVIHGKGLGKKLGVPTANIRPMDKYVIP
                     KKGIYMTYTVIDGKTYPSATNIGVNPTFDEVDLKIENHIIGFDDEIYGKQIEIFFLEY
                     IRPEKKFDSVEDLKSRMKEDIRYVKQKSDLQL"
     gene            11719..11985
                     /gene="rpsO"
                     /locus_tag="VFD79_05785"
     CDS             11719..11985
                     /gene="rpsO"
                     /locus_tag="VFD79_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HZH93595.1"
                     /translation="MLTREEKDNIIEEYKMHEGDTGSPEVQVALLTHRINSVNEHLQS
                     HKKDHHSRRGLLKMVGQRRNLLRYLQNKDIERYRSLIEKLGIRG"
     gene            12068..14185
                     /locus_tag="VFD79_05790"
     CDS             12068..14185
                     /locus_tag="VFD79_05790"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HZH93596.1"
                     /translation="MERKFHYTLAGRELTVTTGKVAEQASGACLVQYGDTVTVVTATQ
                     SKKPKEGLDFFPLSCDYEEKLYSVGKIPGGFIKREGRPSEKAILTSRLIDRPIRPLFP
                     DGFRNEVQVVATVLSVDQDSSPEVSAMIGSSIALSISDIPFDGPTASLNIGLVDGEIV
                     INPDSKQREVSELDLVVSGTKDAIMMVEAGAKFVSEETMLRAILAGHAEIKKLCEFIE
                     GIAAEVGKEKVEYQVFLPDEEIRDKVEEIGRDKLAQALNTEDKVEREEKLSLVKDEIL
                     LEALESFPEGEKHIKEVIGDIIKDNVRRLITEESKRPDNRELDEIRKISTEVGLLPRV
                     HGSGLFNRGQTQVLTIATLGASSDVQIIDGLGDDASKRYMHHYNFPAYSVGETRPMRG
                     PGRREIGHGVLAERALEPVIPSEDDFPYTLRLVSEVLSSNGSSSQASVCGSTLALLDA
                     GVPIKAPVAGIAMGLIKENDNVAILTDIQGLEDHLGDMDFKVAGTREGITAIQMDIKI
                     SGISEEIMREALEKARVARLYILDKMAETIAQPREEMSPYAPRIQTLQVHPDKIRMII
                     GPGGKVINKIIDETGVKIDIDDTGKVAIVSNDGEGSRRAIEMIETIIKEVEAGETYKG
                     KVTKLMNFGAFVEVLPGKEGLVHISQLAKERVNKVEDVVAVGDEIMVKVMEIDKQGRI
                     NLSRKVLLKDEPKTETETENTDK"
BASE COUNT         4904 a         2441 c         3461 g         3504 t
ORIGIN      
        1 gagtttgaga gttgattaag gagagttaag gcatggcaga aaatagaact ataaaatcat
       61 tgttctctga cgatactcag atcaagtctt ttctggaaga tttgagtatc atttccgtag
      121 ttcttgataa taagtccttg aaattgtcaa tcaagatgga ggacaggcat tgggtgaccc
      181 aaaaggacaa agcatcgata gcatctgttt tggaggagaa gttcaaggga ttagctcttg
      241 atattgatta tacaaagata gaagctaatc ttaaccagga tcaactcctg gacataatta
      301 aaagaaaaat aagtcagaac ataccctcta gctcttcgtg gttagaggat gttgttttga
      361 atgagggaga tcttataaac ctcgtattgc caggtgacct gccctacaga tcagtgacca
      421 gaaatggtct gcttgaagag ttgactgaga tttccaaaaa gtcaacaggg aagaatttga
      481 atgtggattt catggatacc aaggaacacg aaggattcct atttgatgca gaaatggaag
      541 agcgcaagat actgaaggaa atgatgctaa aaaagcctgc tgaaaaatca gagaaaaaag
      601 ccagaaacaa ggattattcc tttggaaaga gaattaaggg tgacccaatc aagataattg
      661 acatcgatat gcagactgga agtgcaataa tcgagggtga aatttttcag gttgaaacaa
      721 aatccattaa ggataaccga acactggtaa tattctactt gtcagatggg actaattcca
      781 tttcatccaa ggtcttccta aacggagacc agactgagga gttcacaagc aatattaagg
      841 agggaacctt tgtccttcta agtggggatg tggtatacga cacctatgct aaggaaatga
      901 ccataatgct taagtccatc caggctaagg aacaggagat cataaaggat aatgcaaaag
      961 acaagagggt tgagcttcat ctacatactc agatgagttc aatggacggg gtatcacaca
     1021 ccagggattt gataaagcgg gccatagagt gggggcatga agccattgcc attactgatc
     1081 atggtgtagt acaggctttt cctgatgcca tggaaatgag caaggacatt aagatcatct
     1141 atggggtcga ggcttacatg atcaatgata agaagcagat tgtaacagga aatctcgaag
     1201 gaagaacact tgatgatttt gttgttttcg accttgagac tacgggactt tcgccgaaaa
     1261 atgatagaat aactgaaatc ggagccgtga aaattcagaa tggagaaata atcgatagct
     1321 tcagccaatt gatcaaccct catataccca tacccgacct aataacaagg ctaactggaa
     1381 taacagatga gatggtcaag gataagcctt caatagggga ggttctgcca gaattccttg
     1441 agtttattaa ggatagtgtt ttggttgccc ataatgcatc cttcgacatg gggtttattc
     1501 gggaagcata tgaaagcatc gagattcatc tggacaaccc tgttatggat acacttgagc
     1561 tttccagagc catgtttcca acaatgaaga gccacaagct aaacctggtg gctaaacatc
     1621 ttaaggttga gcttaagaac catcacagag ctgtggatga cgcactggca acaggaaata
     1681 tcctattgag gattttggag ttgttaggtg atagggattt gaaaaatttg gaggatatca
     1741 atggcctgac aggcaatgcg gattccacaa aaggggatat gcatcacatc atactccttg
     1801 caaaaaacca gtctggctta aaggacctat acaggttgat atctgaatca catctgaact
     1861 attttcaccg gaagccaagg atgcccaagt cactaattga aagtcacaga aaggacctta
     1921 ttgttggaag tgcctgtgaa gcaggagaac tatacagggc aataataagg aataagccag
     1981 atcaggaaat aagagatatt gcaaaatttt atgattatct ggaaatacag cctttgggaa
     2041 ataacgagta catggttaga gaaggaattg tgaagaacca ggatgagctg aaatccataa
     2101 acaaaatgat ctataagttg ggtaaaagct taaacaagcc agttgttgca actggggatg
     2161 ttcacttttt agatcctgaa gacgaggttt acagaaggat tctaatgtct ggtcagaaat
     2221 tccaggatgc agataatcaa ccaccattat actttaggac cacagaccag atgctaaggg
     2281 aatttgatta tatgggtgaa gaaatcgcca aggaggttgt aattacaaac cccaacaaaa
     2341 ttaattccca gatagacaag tgcataccaa ttccagatgg tacattccca ccagaaattg
     2401 agggttcaga ccaggaattg agagagatta ccataagcaa ggctgtagat acctacggca
     2461 gcccacttcc cgacctggtt aaaaagagat tggagaggga attggactca attataaaaa
     2521 atggctacgc agtaatgtac atcatcgctc aaaagttggt gtggaaatcc atggaggacg
     2581 gatatttggt cggctcaaga gggtctgtag gatcttcctt tgctgcaaca atgagtggaa
     2641 taacagaggt aaacccactt ccgccacatt atgtctgccc ctcatgcagg aatagtgagt
     2701 tcttcacaga cgggtcaatt gcatcaggct atgatttgcc cgataagcga tgtccaaaat
     2761 gcgatgaaga ctacatcaag gaaggacacg atattccatt tgaggtattt ttgggatttg
     2821 aaggggacaa ggagcctgat attgacctta acttcgctgg agaataccaa cctagagctc
     2881 ataagtacac tgaggagctt tttggtgaag gaaaggtctt tagggctgga acaataggaa
     2941 caatagcaga caagactgcc tatggctttg tcagaaagta ctttgaggaa agggaaatga
     3001 acgtcagcca agcagaaata aataggctct taagagggtg tacgggcata aagaggacat
     3061 ctgggcaaca tccaggagga gttatgatag ttccagatta caaggatatt catgatttct
     3121 caccgataca atatcctgca gacgataaaa aatcaggagt gataacaacc cattttgact
     3181 accatgcaat aagtggcagg atactgaagc tggacatact aggtcatgat gttccaagta
     3241 taataagaat gcttgaggac ataaccggaa ttgacccaac aacaatccct cttgatgaca
     3301 aggataccat ggagttgttt acaagcacca aatcaatagg agtagaccca gactccatta
     3361 actgcccagt gggcaccatg gggattcctg aatttgggac aaaatttgta agacagatgc
     3421 taattgaaac aaaaccaacc actttttctg aacttgtcag gataagtgga ctgtctcacg
     3481 gaactgatgt atggatcaac aatgctcagg agcttgtaaa aaacaaagtt gcaccattgg
     3541 ccaaggttat atccacccgt gaggacataa tgctttcctt gataaaggca ggtatggata
     3601 agaagcaatc ctttttcatc atggaaaagg taagaaaggg aaagggtctt aagccggatg
     3661 aagaggaggc catgagaagt ctggggatac ctgaatggta cattgaatcc tgcaataaga
     3721 tcaagtatat gttccccaag gcacatgctg ccgcatatgt gacgatgagc ttcaggatag
     3781 cctatttcaa ggttcaccat cctcaagcct tctatgcaac ctatttcaca accaaatcca
     3841 ctgacttcga tgcagatctc atactaaagg gagaggcagc tgtcagacaa aagcttaagg
     3901 aactggagca aaaaggtatg ggtgccacaa caaaggagaa aaaccagata actgtgctgg
     3961 aggtagttct ggaaatgtat accagaggct tcacctttga cagggttgat ttgtacagat
     4021 ctgacagcga caggttcctc ataaatgaaa agggtatttt accaccactt aaggcactgg
     4081 atggagtagg tgaaaacgcc gcaagaaaga tagctgagga aagagagata aaggtgttta
     4141 tgtccataga ggatctggtg aaaaggggta aagcaacaag accggttata gatgctctaa
     4201 agggtcatgg atgtttggat tcactgcctg agagcaatca aataagctta tttaacattt
     4261 gatttaatga ctttaatgtg ttataataga caagacaatt agagtgattt ccggcaagag
     4321 tgggtttttc ccactcttcg tttgtgtcag ggagtctgat atggaggtgt atgatgagta
     4381 aaaaaggttt tatgacaagc attgaaaatt tatgtgagga tgtcacttca gagctgggct
     4441 atgaacttgt cgatgtggaa tacattaagg aaagtggcga ctactaccta agggtgtaca
     4501 tccataagcc tggtggagtg aatcttgatg attgccagat tgtcagtgaa aagctcagtg
     4561 aaaagcttga tgacagtgat atgctacaga aagcttatta tctcgaggta tcatccccag
     4621 gacttgacag accactgaag acagacaggg acttggaaag aaatctcggc aaggaagtag
     4681 aggttaacct ctataaggct gtagacggag taaagaagtt tgaagggatt ttaaagtcat
     4741 ataagggaga ttcagtagag ctggaaaccg tagataaagt ggttgaaata cccagggata
     4801 ccgtatcctt gattcgttta accattaaat tttaaggagg aatataagga tgaatgttga
     4861 tttcatcaat gctcttgagg aaattgaaag agagaagggc atttctaagg atattatttt
     4921 tgacgcttta gagtcagctt tgatatccag ttataagaag aattttggtt cttcacaaaa
     4981 cgttatagtg gaaatggata gacaatcagg ggatgtggag gtatttgcaa caaaagacgt
     5041 ggtagagaat gaccatattg aagatgaact tttacaaatg gcccttgaag aagccaaaga
     5101 gatcaatcca aactacgagg ttggagatgt ggtgaagata gcgatcacac caaaggactt
     5161 tggaagaatc gcagctcaga ctgctaagca ggttgtaatc caaagaatca aagatgctga
     5221 gagagacgtg atcttcgatg agttcataag cagggagaat gaaataatta ctggccagat
     5281 tcagaggatt gcaaagaata atgtgtatat cgacctgggt aaaaccgaag gaatacttcc
     5341 accatccgag cagattgcag gtgaggagta cggccaggga gacagaaaga agcttttgat
     5401 tctggaggtc aagaaaacta ctaagggacc tcaaatcgta ctctcaagag ccaatactgg
     5461 acttgtaaag aggttgtttg agctggaggt tcctgagata gctgagggga ttgttgaaat
     5521 ctattcagtt tcaagggaag caggttcaag aacgaaaata gcagtattct caaagaaccc
     5581 agatgttgat gccttgggat catgtgtagg ttttaagggg gcaagagtaa aggcaatagt
     5641 ggatgaactc aatggtgaaa aaatcgacat cgtaatttgg agtaaagagc cagcagaatt
     5701 cataggcaat agcctgagtc cttcaaaggt ggtcaaggtt gttccaaacg aaaaagagaa
     5761 atcggcaata gtggttgtac ctgactacca attgtcccta gccataggaa aagaaggaca
     5821 gaatgccaga ttggcagcaa agcttaccaa ttggaagatt gacataaaat cagaaaccca
     5881 gtacaaggaa ttcatggaaa agaaggccgc tcaggaagaa caggttgctg tagatgaaga
     5941 tgttgaagtt gatttacttg ttgatggatc agctacttat cctgaccaga cttcagatga
     6001 aaactcagag gagggtcttg atgaaatatc aagtgaaact tctgacgaat cctcagatga
     6061 agagtagata ggaggaatac catgagcgca agaaaaatcc cattgagaaa atgcatagca
     6121 tgtaatgaga tgaagcctaa aaaagagctt gtaagagtgg ttaagaacaa ggaaaacgaa
     6181 gtaagtgttg atctaaccgg aaaggccaat ggaagaggag cttatatctg tccaaccatt
     6241 gagtgctttg aggaggctta caggaataaa aaattccaaa gggccttaca gactgaaata
     6301 actgaagaag tgtactccgg gctgaagaag gtcatagata aataaaggat caccttaaac
     6361 aaacaggagg tgcaaggtat gacaaaagta agaatttatg aactagcgaa agaacttaat
     6421 atgtccagca aagccttaat ggaaaaaatc gaaggacttg aattgaagat caatagtcat
     6481 atgagttcta ttgaggatga agaagcacag cttataaagg aactgttgac tgaggacaag
     6541 acagaaggca aagctgtaat tgaagatgag cttgagattt ttgacgagga cgaaaagcct
     6601 aagtcaaaaa aaccaaagaa aaagcagcat aaaatagaag agactccaat agaaaaagag
     6661 gaaaaaatta tcgaaaagaa gactattgaa atagaatcag aagttattgt aaaagacctg
     6721 gctgaaatga ttggggttaa ttcctcccaa atcataacaa agctaatcgg actgggtgtt
     6781 atggttaatc aaaatcaagc aatagacagt gagattgctg ccattgtggc agatgatttc
     6841 aacgttgagg taaagaccct tgagcctgag gttgagctaa cagaggaaga cctattggat
     6901 tatgacgaca aacctgagga catggtggct agaccacctg ttgtaaccgt tatgggtcac
     6961 gtagaccatg gtaagacatc cttgctggat gccattagac acaccaatgt cactgataag
     7021 gaagccgggg gaataaccca gcatattggt gcatatgcag taaacatcaa caataagaag
     7081 atcgtcttcc tggacacacc tggtcatgaa gcctttactt caatgcgagc gagaggtgca
     7141 agcgtaacgg acgttgcaat tctagttgta gcagctgatg acggagtaat gcctcagact
     7201 gtggaagcca tatcacacgc aaaggctgcc ggtgttccaa taatagtagc aatcaataag
     7261 attgacaaac cggattcaaa tcccgataga gtgaagcaag agcttgctga taatggactt
     7321 gttccagagg actggggtgg ggatacaata attgtacctg tttcagctat tcaaagaaca
     7381 ggtatagaag aattgcttga aatgatattg ttggtggctg acatgcagga attgcaggcc
     7441 aatccagaca gaagagctgt tggtacaatc atcgaggcac agcttgacaa ggggaaagga
     7501 cccttggcaa cagtacttgt tcaaaagggt acacttcatg ttggggacat ggttgtatca
     7561 ggtacttcaa gcggccgtat ccgtgctatg cttgatgaaa agggcaaaaa cgtcaaaaag
     7621 gccactcctt caatacctgt aatgattttg ggtctatcag aagttccaaa tgcaggagac
     7681 ctattatttg cagttgaaaa tgagaaggct gcaagaaata ttgcagatgc aagaaagact
     7741 ctgattagag aagcgcaaat gaagcagtca tcaaaggttt cactggatga cctttttgaa
     7801 aagataaaac ttggtgagat caaggaccta aacattatcg taaagggtga tgtaaggggc
     7861 tcaatagaag ccctaagcca atcactgaac aagcttgaca ctgaagaggt aagggtgaat
     7921 cttatccatg gtggggtcgg tggaatcact gaaagtgata ttatgctggc atcggcttca
     7981 aacgccattg ttattggatt caacgtaaga cctaacctta atgccataga ggttgccaag
     8041 aaagaggatg ttgatgttag aacctacagg atcatatatg aggccataga agacatccag
     8101 gctgcagtca agggaatgca cgcgcctaag atagtggagg agatccttgg acgagctcag
     8161 gttagggcga ccttcagact gcccaatgga aacacaattg caggtattta tgttgttagt
     8221 ggaaaaatca ccagaaatgc aaatattagg ctcctaaggg atgatgttgt tatattcgat
     8281 ggctcagtat catcgctaaa gagattcaag gatgatgcca gagaggtcct gaccggtttt
     8341 gaagctggtt taggtcttga aaacttcaat gacatcaaag agggagacaa tcttgaggca
     8401 tatgtcctta aggaagtcaa aagagattag aggtgtaata gatgaacgaa aagagaataa
     8461 acaggatttc cgaggaagtt aaaaaagtcg tatcagacct tattcaaaac gagatcaagg
     8521 atcctaggat cagtgggatg ccaagtgtca acaaggtcag cgtcaccaag gacctaagat
     8581 ttgccaagat atatatatct gttttgggaa gtgaagagga taaggaaagt accatagaag
     8641 gacttcaaaa tgcaaaaggc tttgtgagaa aggaaatcgg aaacagaata aaccttaggc
     8701 atgtgccgga accgattttc cttctggacg aaagtataga gcaggcttta tatatgtcca
     8761 agctgattga agaagttaac aggggctcaa agaaggactc tgatgaaaat gaagaataat
     8821 cttgaggcct ttaaagagct tatcaggacc agtgaaagga tagccgttgt ctcccatatg
     8881 aatccagatg gagacaacct gggctccctt ttggctgctg gcctgtctct tgaaaagatg
     8941 gggaaaggcg tcctcttcgt aaaagctgac ataatacctg aggactacag gttccttcca
     9001 ggtgtggaaa gactatcacc tgttgatgaa acaattgaca tgcttgacct actcttggta
     9061 ttggacagca gtgatccaga cagacttggg gagaacaggt ttctaattga caaatcacag
     9121 aaggttgtaa atattgacca tcatgtaagt aactctgagt ttggagatat caacatagta
     9181 aaacctgaaa tgtcatctac cggtgaaata ttatttgaat tgctgctgca gatggatatg
     9241 ccaattgata gtgagatcgc ttcactaata tatgttgcaa tgagcactga cactggtaga
     9301 ttcacctatc agggcgttac tggcaaaact catgaaacag ttgcaaggct atatggatac
     9361 ggtattgatg catatgcaat aaataacagt ctttaccaaa ggagaagtct ggaaagaaca
     9421 aaactattca taagggctgt gtcaaatatc aggttgtata aggacaacag gattggtatt
     9481 gtcagggtta caaaatccat gttggatgaa accggatcat caatggaaga tactgaaggc
     9541 attgtggagt tcatacgaga tacagattct gtagaggctg catgcattct aaaggaaatt
     9601 gatgatggtg tagtaaagat tagcctacga acaaaaaata tagtagatgc aaacaaggtt
     9661 tgcagtgtat tccatggcgg tggacacagc agggcatcag gagccacggt ggaggataca
     9721 atagacaatg ttgagagtag actactggac gaaataaata aatatctata gataagcagg
     9781 gtgagtggat gaatggaata attaactttt tcaaaccagc tggtatgact tctcacgatg
     9841 ctgttgctta cttcaggaga ttgttgggca caaaaaaagt tggccatact ggaacattgg
     9901 atccaatggc aacaggcgtt ctcccaattt gtgttggtaa gggtacaaag gtctcggaat
     9961 acctccttgg agttgataag gagtatatag ctgagcttac cttggggact tcatcagata
    10021 cccaggatag tacgggttct gttataaatt cttcagataa agttgtggac aatgaccaga
    10081 tcgaaaatgc atttcattcc ttccagggcg agatcgacca gattccacct atgtattcag
    10141 caaagaaaat aggaggaaaa aaattgtatg aacttgccag aagtggcatt gaggtagaga
    10201 gaaaaccaag gagaatcaac ataaaggaaa tcaggattct caacaaccag gacaaccgta
    10261 aaattatctt ctataccaag tgttccaaag gaacatatat cagaacaata tgcaatgata
    10321 ttggagacct tcttggaacc tatgggcaca tgtcatacct acttagagta ggggttggga
    10381 ccttcagggt agaggattcc tatggaatgg ataccctaaa tgaaatggac aaggaaggcc
    10441 ttttaagtat aatccagcca attgacaatg caatccaatt catggatggc atcaatctgg
    10501 actccaagtt ctataaacca ttgaccaatg gtgttagaat tgagttggac aaaaatttga
    10561 aagtagatac agacaagctc catagaatat atgtttctga cgaatttatc ggcattggaa
    10621 gcatccttga agaaaatggc agaaaaacac taaaaatgga aaaagtactg atcaggtgag
    10681 aaaatggaaa taataagaat tgacaataca aatgaaacca ggttccagac ttcccttgct
    10741 ttgggtaact ttgacggtgt tcacattggc caccaaaaac ttattagatc aatgatagaa
    10801 accgcaaacg aatctggttt tcagccttca atgatgatat ttgaaaacca caccaagtca
    10861 tttgtgttta agaatgggcc tagactgata agcagcaatg atcagaaaaa taggtttctt
    10921 gaaaagcttg gtgttaaaac cctttatact atggtatttg acgatgaaat aatgactctt
    10981 agtcctgagg aatttgtcac gtccatctta tatgagaagt taaactgcag agcaataacc
    11041 gttggacctg actatagatt tggttacaag gctcaaggga atgttgacca actgatttca
    11101 ttgagcaggg aactgggcat aaaaactgat attatccaac ctgtttacca taatgacata
    11161 cttgtaagca gcacccgggt gagagaacat ttgacagacg gggaaattct cgaagccaac
    11221 acactattgg gaagaccctt cagtgtaatc ggggaggtca ttcacggtaa aggtctgggt
    11281 aaaaagcttg gagtccccac tgcaaacatc aggccaatgg ataaatatgt aatacctaag
    11341 aaggggatat acatgaccta tacggtcata gacggaaaga catacccaag tgcaacaaat
    11401 atcggtgtaa acccaacatt tgatgaggtg gaccttaaga tagaaaatca catcattgga
    11461 tttgatgatg aaatctatgg caagcaaata gagattttct ttcttgaata tataagacct
    11521 gaaaagaagt ttgattcagt ggaggacctt aaaagccgaa tgaaagaaga tataaggtat
    11581 gtcaaacaaa aatcagattt acaattatag taaagtatgt taaactttat atgttaagga
    11641 ttacctttgg caatgcttgt cgttcctcgg cgcctgcata gctgatggtg gaccaataaa
    11701 ttatttggag gtgcaaaaat gttaacaaga gaagaaaaag acaacattat cgaagagtat
    11761 aaaatgcacg agggtgatac aggatcacca gaagtacagg ttgcactgct tactcacagg
    11821 attaattctg taaacgaaca cttgcaatca cacaagaaag accatcactc aagaagaggt
    11881 ctacttaaaa tggttggaca gagaagaaat ctactaagat atttacagaa taaggatatt
    11941 gaaagatatc gttctttaat tgaaaaactt ggcatcagag gttaattaga gcgggacttc
    12001 ccgctctatt agtttatttg actatataat atgacacaca ctttaagaat ttacaaggag
    12061 gtaatttatg gaaagaaaat tccactacac cttggcagga agagagttga ctgtaaccac
    12121 cggtaaagta gctgagcaag ctagcggagc ttgcctggta caatacgggg acaccgttac
    12181 ggttgtaaca gctacacaat ccaagaaacc aaaagaaggt ttggatttct tcccgcttag
    12241 ctgtgattat gaagaaaaac tttattcagt tggaaagatt ccaggcggat tcatcaaaag
    12301 agaaggtagg ccaagcgaaa aggccatttt gacatcaaga ctaatagaca gaccaataag
    12361 acccttgttc ccagacggat tcagaaatga agtgcaggtt gttgcaacag ttctatcagt
    12421 agatcaggac agcagtcctg aggtttctgc tatgattggg tcatcgattg cattgtcaat
    12481 ttccgatatt ccatttgacg gacctacagc atcactaaac attggtttgg ttgatggtga
    12541 aatagtcata aacccagaca gcaagcaaag ggaagtttca gaactggact tggttgtatc
    12601 aggaacaaag gatgctatca tgatggttga agcgggtgcc aagtttgttt cagaggagac
    12661 aatgctaagg gccatacttg ctggtcatgc tgagatcaag aagctatgcg agttcataga
    12721 aggtattgct gctgaagtag gcaaggaaaa ggtagagtac caggtcttcc tgcctgatga
    12781 ggaaatacgt gacaaggtag aagagattgg cagggacaag cttgctcaag cccttaatac
    12841 cgaggataag gttgagagag aagaaaaatt atcattggtt aaggatgaaa ttctcctgga
    12901 ggcattggag tcatttcctg aaggtgagaa acacataaag gaagtaatag gtgacataat
    12961 caaagataat gtaagacggt tgataacaga agaatcaaag agaccggaca accgggaatt
    13021 ggatgaaatt agaaaaatca gcactgaagt tggacttcta ccaagggtac acgggtccgg
    13081 cttgttcaac aggggccaga ctcaagtcct tacaatcgca actcttggag cctcaagcga
    13141 tgtacagata atcgatggtc ttggcgatga tgcatccaag agatatatgc accattacaa
    13201 tttcccagct tactctgttg gtgaaaccag acctatgaga ggaccaggca ggcgtgagat
    13261 cggacatggg gtattggctg agagagctct cgagccagta attccttcag aggacgattt
    13321 cccatataca ctaaggcttg tatctgaagt tcttagctca aatgggtcat cttcacaggc
    13381 aagtgtctgc ggaagcaccc tggcacttct agatgcaggg gtacccatta aggcaccagt
    13441 agctggaatt gctatgggct tgataaaaga aaatgacaat gttgccatat tgaccgacat
    13501 tcaaggtctt gaagaccatc tgggagatat ggacttcaag gtagccggaa caagagaagg
    13561 cattactgct atccagatgg atatcaagat ttcaggcatc agcgaagaaa tcatgagaga
    13621 ggcacttgag aaggctagag ttgcccgact gtacattctt gataaaatgg ctgagaccat
    13681 tgcacagcct cgagaagaga tgtcgccata tgcccctaga atacagacgc tgcaggtaca
    13741 tccagataag atcaggatga ttatcggacc tggaggaaag gttatcaata agattattga
    13801 tgaaactggg gttaagatcg atattgatga taccggaaag gttgccattg tatctaacga
    13861 tggagaaggt agcagaagag ccattgaaat gatagagacc attatcaagg aagttgaagc
    13921 cggtgagacc tacaagggta aagttaccaa gcttatgaat ttcggagctt ttgtagaggt
    13981 tttaccaggt aaagagggac tagttcacat atcacaactt gccaaggaaa gagtcaataa
    14041 ggtagaggat gttgtggctg tcggagatga gattatggta aaagtcatgg agattgacaa
    14101 gcagggtagg atcaaccttt caagaaaagt actccttaag gatgaaccaa agacagagac
    14161 agagacagaa aacacggata agtagaacca gaacatgaac cttaaaccgg ttcatgttta
    14221 tttttgttaa caaatatacc aattattgat aaaatagaat ttgagatcag cattcggagg
    14281 aaaatatgca tactataaat cgtctagaca
//
LOCUS       DASRQF010000218         5148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1498057, whole genome shotgun sequence.
ACCESSION   DASRQF010000218 DASRQF010000000
VERSION     DASRQF010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5148
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1498057"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /locus_tag="VFD79_05795"
     CDS             complement(<1..1191)
                     /locus_tag="VFD79_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HZH93597.1"
                     /translation="MVHPVTFGKRVRMSYSRIDEVLDLPDLIEVQKSSYDWFLSEGLR
                     EVFDDISPIQDYTGNLILEFVDYHIGEAVKYDEHEARERDANYSAPLKVKVRLINKET
                     GEVKEQEVFMGDMPLMTDKGTFIINGAERVVVSQLVRSPGVYYVESIDKSGKSLFSST
                     LIPNRGAWLEYETDSNDIVYVRIDRTRKVPITTLMRALGIATDSEVVDVLGETEQLLT
                     TLEKDATNTEEEALLEIYKRLRPGEPPTVDSARSLLTNLFFDPRRYDLAKVGRYKFNK
                     KLALYNRVAGNRAAHDVVNPETGEILAEKGERISRDKAIEIENSGINSMKLLMDDGKE
                     IKVLSNNFVFPEAFDLPFSLKELGLRERVYYPLMKELIEAFPEEEELKDAIKDRIKEL
                     SPKHI"
     gene            complement(1425..1805)
                     /gene="rplL"
                     /locus_tag="VFD79_05800"
     CDS             complement(1425..1805)
                     /gene="rplL"
                     /locus_tag="VFD79_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017150119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HZH93598.1"
                     /translation="MASEKVLNLIEEVKSLTVLELSEVVKALEEEFGVSAAAPAAVAA
                     APAAAAEAAVEEKTEFDVILASAGGEKIKVIKAVREITGLGLKEAKELVDNAPKAIKE
                     GASKEEAEELKAKLEEVGASIEVK"
     gene            complement(1839..2366)
                     /gene="rplJ"
                     /locus_tag="VFD79_05805"
     CDS             complement(1839..2366)
                     /gene="rplJ"
                     /locus_tag="VFD79_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HZH93599.1"
                     /translation="MKEHTLQAKSKVVEEIKEKIQSSQSMILVDYRGLDVAELTELRK
                     QYREAGVDYKVYKNTMMRFAFKDLGLEEFNEFLKGPSAVAFGLEDPVAAAKVTSEFAK
                     KNDKLEIKAGIVDGKIIDVNGVKSLADLPSKEVLVAQVLAGMNAPVQGFANVLQGTIR
                     SLAIVLNAIAEEKAA"
     misc_feature    complement(2386..2513)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(2549..3247)
                     /gene="rplA"
                     /locus_tag="VFD79_05810"
     CDS             complement(2549..3247)
                     /gene="rplA"
                     /locus_tag="VFD79_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HZH93600.1"
                     /translation="MPKRGKNYQESMKLVDRNKLYDTLDAIELVQQTSKAKFDETVEL
                     AVRLGVDPRHADQQVRGAVVLPHGTGKTSRVLVIAKGDKVKEAEDAGADFVGGDDMVA
                     RIQNEGWMDFDVVVATPDMMGVVGRIGRILGPKGLMPNPKSGTVTFEVEKAVNEIKAG
                     KVEYRVDKASIVHVPIGKVSFGTDKLRENFVAIMDALNKARPAAAKGRYMRTVTISST
                     MGPGIKLNPGKLTD"
     gene            complement(3297..3722)
                     /gene="rplK"
                     /locus_tag="VFD79_05815"
     CDS             complement(3297..3722)
                     /gene="rplK"
                     /locus_tag="VFD79_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002835316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HZH93601.1"
                     /translation="MAKKVIALVKLQIPAGKATPAPPVGTALGPHGVNIMQFTKEFNA
                     KTADQAGMIIPVVLTVYADRSFTFITKTPPAPVLIKKAVGIDSGSGEPNKKKVAKISK
                     ADLEEIAKIKMPDLNAGSVEAAMSMVAGTARSMGVEVEE"
     gene            complement(3836..4390)
                     /gene="nusG"
                     /locus_tag="VFD79_05820"
     CDS             complement(3836..4390)
                     /gene="nusG"
                     /locus_tag="VFD79_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HZH93602.1"
                     /translation="MTDKDMSREERAKDAKWYVVHTYSGHENKVKANLEKMVENRGMK
                     DDIFEVAVPTEEYIDNKSGKKKLKERKMFPGYVLVKMIINDESWYLVRNTRGVTGFVG
                     PSSKPVPLSDKEVRALGVQDKMVLPAVDFKVKDPVKVVAGPFENFIGSIDSINMEKRK
                     VKVFVSMFGRETLVELEFDQIERV"
     gene            complement(4430..4642)
                     /gene="secE"
                     /locus_tag="VFD79_05825"
     CDS             complement(4430..4642)
                     /gene="secE"
                     /locus_tag="VFD79_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009431823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HZH93603.1"
                     /translation="MAGSNTGSVSTKDKMSTYFRGVRSELKKVIWPDKKELVNYTGVV
                     IFISVLVAVIVYILDLILGGILSFII"
     gene            complement(4667..4816)
                     /gene="rpmG"
                     /locus_tag="VFD79_05830"
     CDS             complement(4667..4816)
                     /gene="rpmG"
                     /locus_tag="VFD79_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HZH93604.1"
                     /translation="MRDRVTLACTECKQRNYVTKKNKKNTTERIELKKYCKFCKAHTN
                     HKETK"
     gene            complement(5012..>5148)
                     /gene="tuf"
                     /locus_tag="VFD79_05835"
     CDS             complement(5012..>5148)
                     /gene="tuf"
                     /locus_tag="VFD79_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269603.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HZH93605.1"
                     /translation="VMPGDNARFSIELITPIAMEEGLRFAIREGGRTVGAGVVTKVYE
                     "
BASE COUNT         1256 a         1304 c          852 g         1736 t
ORIGIN      
        1 tatgtgtttt ggtgacaatt cctttattct gtcctttatt gcatccttaa gctcttcctc
       61 ttctgggaac gcctcaataa gctccttcat aagggggtag tatacccttt ccctcaatcc
      121 aagctccttc agggagaatg gaaggtcaaa tgcctccggg aatacaaagt tattggaaag
      181 aaccttgatt tccttgccgt cgtccattaa cagcttcatt gaattgattc cgctgttttc
      241 aatctctatt gccttgtccc tgcttattct ttcacccttc tcagccagga tctctcctgt
      301 ctcagggttg actacgtcat gagcggctcg attacctgca actctgttat aaagggctaa
      361 tttcttattg aatttgtatc tcccgacctt ggcaagatca tatcttcttg gatcaaagaa
      421 aagattggtc aacaagcttc tggcactgtc cactgttggt ggttcgccag gtctaagacg
      481 cttgtagatt tcaagaagtg cttcctcttc agtgttggtg gcatcctttt ccaaggttgt
      541 cagaagctgc tctgtttcac ccaatacatc caccacttca ctgtccgtcg caatcccaag
      601 agctctcata agtgtggtga ttggaacctt tctggtcctg tctattctta catagacaat
      661 gtcattggag tcagtctcat actccagcca cgctccacgg ttaggaatca aggttgatga
      721 aaacaggctc ttgcctgact tatcaattga ctcaacataa tatactcctg gtgatcttac
      781 cagctgactt acaactaccc tttccgcacc gttaatgata aatgttccct tgtctgtcat
      841 caaaggcatg tcgcccatga agacctcctg ttcctttacc tcaccggtct ccttgtttat
      901 aagtctaacc ttaaccttca agggtgctga atagttagcg tccctctctc tagcctcgtg
      961 ctcgtcgtac ttgacggctt ctccaatgtg gtagtccaca aattccaaaa tcaggttacc
     1021 tgtgtagtcc tgaattggtg agatatcgtc aaaaacctct cttaatcctt ctgacagaaa
     1081 ccaatcgtag gaggatttct gcacctcaat caaatcaggc agatcaagta cttcatcgat
     1141 gcgggaataa ctcatcctga cccgcttacc aaatgttaca ggatgtacca ttaaaacttc
     1201 acccctcatt aaactaaaat agccaaaagc cacggctttt ggtggtacca actcatcggt
     1261 caactgaaaa taacaggttc taaatatcag tatagtatgc ccattataat cattattatg
     1321 caatatatta tactatcata cacacctggt gatgtcaaat cttttaggtc atttttagtg
     1381 aaaaaaagtg gccttcatga tgaaagccac tttctattct tcaattactt aacttcgatt
     1441 gatgctccaa cttcttcaag cttagcctta agctcttcag cttcttcttt gcttgcgcct
     1501 tccttgattg cctttggtgc attgtctaca agttcttttg cttctttaag tccaagacct
     1561 gtgatttctc ttacagcctt gataactttg attttctctc cgccagctga tgcaagaatt
     1621 acatcaaact ctgtcttctc ttcaactgct gcctcagctg ctgctgcagg agctgctgct
     1681 actgctgctg gagctgctgc acttactcca aactcttcct ctagagcctt tactacttct
     1741 gaaagctcca atactgttaa gctttttact tcttcaatta ggtttaatac tttttcactt
     1801 gccattttta tttatccctc cgaatttttt tattttattt atgctgcctt ttcttcagct
     1861 atagcgttta gtactatagc aagacttctg attgtacctt gaagtacatt tgcaaatcct
     1921 tgtactggtg cgttcatacc agcaagtacc tgcgcaacaa gtacctcttt tgatggtagg
     1981 tctgcaagac tcttgacacc gttaacatcg attatctttc catccacgat tccagccttt
     2041 atctcaagct tatcgttttt cttggcgaat tccgatgtaa cttttgctgc agccactgga
     2101 tcttccaatc cgaaagccac cgctgaaggt cctttaagga attcgttgaa ttcttccagg
     2161 cccaggtcct taaatgcaaa tctcatcatt gtgttcttat ataccttgta gtcaactcct
     2221 gcttctctgt attgctttct aagctcggtc aactctgcta cgtcaagtcc tctgtagtca
     2281 accaaaatca ttgattggga tgattggatc ttctctttta tctcctcaac aacctttgat
     2341 ttggcttgaa gggtatgttc cttcatatta tcttcacctc ctgtacgggt ataaaataaa
     2401 ggtctcatac gcagacacgt agagacccta attccagatt agatatttct cacctcggta
     2461 gggtattaag cctattaagc tcctactgtc tgcggttctt tatttaatta acattgatga
     2521 ttatatcagt aacaacaggt cttgtcaact agtcagtaag ttttcctgga tttaatttga
     2581 ttccaggacc cattgtgctt gaaatcgtta ctgttctcat atatctaccc tttgcagcgg
     2641 ctggtcttgc cttgttaaga gcatccatga ttgccacgaa gttttcccta agcttatcag
     2701 taccgaatga aaccttgcct attggcacgt gtacgatact ggccttgtca actctgtact
     2761 caaccttacc tgccttgatc tcattgactg ccttttcaac ttcaaaagta acagttcctg
     2821 atttagggtt aggcataaga ccctttggtc caaggattct accgattcta cctacaacac
     2881 ccatcatatc tggagttgca actacaacgt cgaagtccat ccaaccttca ttctggattc
     2941 ttgcaaccat atcgtcgcca ccgacgaaat cagcgcctgc atcctctgct tccttaacct
     3001 tgtcaccttt tgctattaca agaaccctgc ttgtcttacc agtaccatgt ggcaaaacaa
     3061 cagcacccct tacctgctgg tctgcatgtc ttgggtctac cccaagtctt actgcaagct
     3121 caactgtttc gtcaaacttg gcttttgaag tctgctgtac cagctctata gcatccaatg
     3181 tgtcataaag cttgtttctg tcaacaagtt tcatgctctc ttgatagttc ttacctctct
     3241 ttggcatact aaaaaacctc cttgtggtat taacgaaaat tttctcccac tatttattat
     3301 tcttctactt caacgcccat gcttcttgcg gttccagcta ccatgctcat ggctgcttca
     3361 actgaacctg cattaaggtc tggcattttt atcttagcta tctcttcaag atcagccttt
     3421 gatattttag caactttttt cttgtttggt tctcctgaac ctgagtcaat tccaacagcc
     3481 ttcttaataa gaactggtgc tggtggtgtc tttgtgataa atgtaaatga tctgtctgcg
     3541 tatacagtta acactacagg tatgatcatt cctgcctggt ctgcagtctt ggcgttgaac
     3601 tctttagtaa actgcatgat gtttactcca tgaggtccta acgcagtacc tacaggtggt
     3661 gctggtgttg cctttccagc tggaatttgt aatttaacta gagctatgac tttttttgcc
     3721 ataattccac ctcctcattt caatgtggtt tagcgggata atccctccca ctgcacttct
     3781 atttataaag catcattgct tataaatcaa tccatcaccg gaccatgttg tccggctaaa
     3841 ctctttctat ctgatcaaac tcaagctcaa ccagtgtctc cctgccgaac atggagacaa
     3901 aaaccttgac cttgcgtttt tccatattga tgctgtcaat ggatccaata aagttctcga
     3961 aaggacctgc tacaaccttt acagggtcct tgaccttgaa gtcaacagcc ggcaaaacca
     4021 tcttgtcctg gactcccagt gctctgactt ccttgtcgga aagcggtacc ggttttgatg
     4081 acggcccaac gaagcctgtt acccctcgag tgtttcggac caaataccat gactcatcat
     4141 tgataatcat tttaacaaga acatatccag gaaacatttt ccgctccttg agcttctttt
     4201 tcccggattt gttgtcgatg tattcttcag ttggaacagc gacttcgaag atgtcgtcct
     4261 tcattcccct gttctctacc attttctcaa ggttggcctt tactttattt tcatgaccag
     4321 aataagtatg caccacatac cacttggcat ccttggctct ttcttcccta ctcatatcct
     4381 tatccgtcat aattcaggga ccactcaggc ccttccctcc cttgtattac taaataataa
     4441 acgataaaat tccacctaga atcaggtcaa gaatataaac tataaccgca accaacacgc
     4501 tgatgaatat tactactccc gtatagttta caagctcttt cttatctggc caaataacct
     4561 tcttaagctc tgatctaact cctctaaagt aagtggacat cttatccttt gtactcacag
     4621 atcctgtgtt tgatcctgcc atcatttcac atccttactt taacagttat tttgtttcct
     4681 tatggttagt gtgtgccttg cagaacttac agtatttctt aagctcgatt ctctcagttg
     4741 tgtttttctt gttcttcttg gttacataat ttctctgctt acactctgta cacgccaatg
     4801 tgactctgtc tctcatctat cacacctcct actttcaccc atggtgttat ctctatatgt
     4861 gaaaacaact aatttgggca caatctccca ttattaatgc actactaata taaactatca
     4921 caaatcatta tttgtgtcaa taaataattc cttctccaca atgatgttaa tgtcataaaa
     4981 agaccgggtc aaaagaccca gtctttcaat cttattcgta aacctttgtt acaacacctg
     5041 ctcctactgt tcttccacct tctctgattg cgaatcttag tccctcttcc atcgcgattg
     5101 gtgtgatcag ttcgattgag aacctagcgt tgtcgcctgg cattacca
//
LOCUS       DASRQF010000219         2418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_745753, whole genome shotgun sequence.
ACCESSION   DASRQF010000219 DASRQF010000000
VERSION     DASRQF010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745753"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VFD79_05840"
     CDS             <1..451
                     /locus_tag="VFD79_05840"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZH93606.1"
                     /translation="GISISDDQVREFYDENKDNLVEINASHILLGSEEDALSIVERLD
                     GGEDFAQLATTESLDSVSAVNGGNLGYFGKGQMIPEFEEVAFQLNEGEISDVVRTEVG
                     FHIIKVQDKRESLEELKDKVNALIMEEEYYEYVQGLKNAATIKTYLD"
     gene            511..2121
                     /locus_tag="VFD79_05845"
     CDS             511..2121
                     /locus_tag="VFD79_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HZH93607.1"
                     /translation="MSKGHSFLRGAAILGMAGVLVKILGAIYRIPLSNIIKSEGLGYY
                     QTAYPFYVLLLTISTAGVPVAIAKLVSEKRAQGDFAGAHKVFKVALVGLVGLGFLTSM
                     VIAFGAGHIVNYLGNVNAYYALIALVPALFFVPIMSAFRGFFQGRQSMFPTAISQITE
                     QLFRVVTGLGLTYLLLDRGIPIAAGGASFGGSMGAIMGLAIIAIIYFKRRAEIMEEIA
                     GSTIRREYSVGEIAKDLLLIAIPITIGAAIAPIMDTIDAALVLRRLQSINYTAAEAND
                     LYGELKGMAQTLVNLPQVFSIAISMSLVPAISNASARGSKQETSDLISSGIRMTLLIG
                     LPSAIGLFVLSTPIVALLYYNNPPEAIASVGNILAHLSLGVIFLTLVQALTAILQGLG
                     KPFVPVRNLFIGAVAKIILTYVLTGIPAINVKGAAISTVAAYAIASTLNLLYVIYVAK
                     AKLNLGAILMKPLVSAVGMGIIARLSYVVLAGPIGGRPATLVAVLLGVIVYGVLLLLT
                     GTLTDEDLDLIPKGDKVRKIVKRFDKWS"
     gene            2134..>2418
                     /locus_tag="VFD79_05850"
     CDS             2134..>2418
                     /locus_tag="VFD79_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZH93608.1"
                     /translation="MSKIFVVGLGPGNIDDLTLGAIRRMEGGKPNYLRTEKHPSVQYM
                     RDRSLEYTSFDHIYDQEASFEEVYETIVSRLIESAREHGEINYYVPGNPVV"
BASE COUNT          711 a          493 c          605 g          609 t
ORIGIN      
        1 cggaatatca ataagtgatg accaagtaag ggaattctat gatgagaaca aggataatct
       61 tgttgagata aatgccagcc acatactgct tggaagtgag gaagatgccc taagcatagt
      121 tgagaggctg gatggaggag aagattttgc acaactggca accacggaat cccttgatag
      181 cgtgtccgcc gtcaacggag gaaacctggg ttactttgga aaaggtcaga tgattccgga
      241 atttgaggaa gttgcattcc agttgaatga aggagaaata agcgatgttg tgagaactga
      301 ggttggattt cacatcatca aggtccagga taagagagag agccttgagg aacttaagga
      361 caaggtaaat gcactgatca tggaagagga atattatgaa tacgttcagg ggttaaagaa
      421 tgcagcaaca atcaagacct accttgacta gcacttcaac cgcatcagga ataattttga
      481 gtcaggaaaa gacacgggag gaaaacacta atgtctaagg gacacagctt tttaagaggg
      541 gcggcaatac ttggaatggc aggtgtactt gtcaagattc tgggagccat atacaggatt
      601 cccctttcca acataatcaa atcagaagga ttgggatatt atcagacagc atacccattt
      661 tatgtgctcc tattaaccat atccacagca ggggtcccgg tggcaattgc caagcttgta
      721 tcggagaaac gggctcaggg agactttgcc ggtgcccaca aggtctttaa ggtggcactt
      781 gtgggccttg tggggctagg cttcctaacc tcaatggtaa tagcctttgg agcgggccac
      841 attgtcaatt acctgggtaa tgtaaatgcc tattatgctt tgatagcact ggtacccgcc
      901 ttgttttttg tgccaattat gtcagccttc aggggttttt tccagggtag acagagcatg
      961 ttccccacag cgatatccca gataacggaa cagctcttca gggtggtgac gggattggga
     1021 ctcacttatc tgctcctgga tagggggata ccaatagctg ccggaggtgc gtcctttgga
     1081 ggatccatgg gagcaataat gggtcttgca ataatagcca tcatatactt caagagaaga
     1141 gcggaaataa tggaggaaat tgcaggaagt accataagaa gggaatactc cgtaggagag
     1201 atagcaaagg accttcttct catagcaata cccattacaa taggtgccgc catagcaccc
     1261 ataatggata caattgatgc agcccttgtt ctcaggagac tgcagtccat aaactataca
     1321 gcggcggagg ccaatgacct ttatggtgag cttaagggta tggcacagac ccttgtaaac
     1381 ctgccccagg tattttccat agccatatca atgagtctag tgcctgcaat ttcaaatgcc
     1441 agtgccaggg gatcaaaaca ggagacaagc gaccttatat caagcggtat aaggatgacc
     1501 cttctaatag gcctgccatc agcaattggc ttgtttgtac tttcaacccc aattgtggct
     1561 cttttgtact acaacaatcc accagaggca attgccagcg ttgggaacat actggcacac
     1621 ctatccctgg gagtaatatt cctgaccctt gttcaggctc ttacagcaat actccagggt
     1681 cttggcaaac catttgtccc agtgagaaac ttattcattg gagctgtggc aaagataatc
     1741 ctgacctatg tccttactgg aattcccgca ataaatgtaa agggagctgc aataagtacc
     1801 gtggctgcct atgcaatagc atcaaccttg aatcttctgt atgtaatcta tgtggcaaaa
     1861 gccaagctta atcttggagc aatactgatg aaacctttgg tatcagctgt aggaatggga
     1921 atcatagcca gattgtcata cgtggttctt gcaggaccaa ttggtggcag accggccacc
     1981 cttgtggcag tacttctggg agtgattgta tatggagtct tgctactctt gacaggtacc
     2041 cttacagacg aagaccttga cctgattcca aaaggggaca aggtccgaaa gattgttaag
     2101 agatttgata aatggtcata aaggagaaaa cttatgagta aaatatttgt tgtaggcctt
     2161 gggcctggaa atattgacga cctgaccctg ggggcaatta gaaggatgga gggtggcaag
     2221 cctaactatc ttagaactga aaaacaccct tcagtacagt atatgaggga taggagcctt
     2281 gaatatacat cctttgacca catttatgac caggaggctt cctttgagga agtttatgaa
     2341 accatcgttt caagactcat tgagtccgca agggaacatg gggagataaa ctattacgtt
     2401 cccggaaatc ctgtagtt
//
LOCUS       DASRQF010000220         7013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1003076, whole genome shotgun sequence.
ACCESSION   DASRQF010000220 DASRQF010000000
VERSION     DASRQF010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 7013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7013
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003076"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      17..126
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            214..702
                     /gene="thiW"
                     /locus_tag="VFD79_05855"
     CDS             214..702
                     /gene="thiW"
                     /locus_tag="VFD79_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HZH93609.1"
                     /translation="MQTKRLVLSGLLVSFGVLASHVIVVPVGVAKAYPLQHTINVIGA
                     VMLGPGYAVANALLISLLRNILGVGSVLAFPGSIFGAMLAGLFYKHFKNEFSAGIGEV
                     IGTGIVGSLVAFPLSRFLLGSETGAFFFIPPFLISSLVGAVLGIFTVKSLSGLGNGVL
                     LK"
     gene            699..1502
                     /gene="thiD"
                     /locus_tag="VFD79_05860"
     CDS             699..1502
                     /gene="thiD"
                     /locus_tag="VFD79_05860"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HZH93610.1"
                     /translation="MKKVLTIAGSDSSGGAGIQGDLKAFSANETFGMSVITAVTAQNT
                     QGVYMVHDIPKEVIQAQIKALFNDVAIDGIKIGMVSIPDTIEMIARTLSLYCLPKIVL
                     DPVMVSKSGSNLLQPAAVNALKEQLIPMAFLITPNIPEAEVLTGIKILSEDDMIMAAR
                     ELMNLGCGGVLLKGGHLDGPAVDILHDGKEITRFEADRISSNNNHGTGCALSSTICSN
                     LAKGYSLPVAVEKGKSYVYESISNGFSVGKGVGALHHFHSFYEGGDHNE"
     gene            1495..2694
                     /gene="cytX"
                     /locus_tag="VFD79_05865"
     CDS             1495..2694
                     /gene="cytX"
                     /locus_tag="VFD79_05865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HZH93611.1"
                     /translation="MNKFSGFNLTALWFGAAISVAEIITGGLLAPLGFKLGLLIIIVG
                     HVIGGLIMVAAGNIGYEMGLASMESTELTFGRYGKTLFSLLNATQLVGWTVIMLISGG
                     AATSVLTRNIFSFGGTSTWIIVLGLLVALWLYAGKGLWKTGNAIIGISMLALTIFIIR
                     IGLANEGITSLPDTNTISIGLAIELTIVMPISWVPLVADYTRDSKSKIGGNLGTFIGY
                     FFGSSLMYFVGLIAALGFGSTDIGSILLNQGYGLVGVLIIMLSTISTTYLDAFSAGLS
                     IKNLVPTLSEKQLGTWVVAISTFIALTFPMTAYESFLLWIGALFVPLFAILITDYYIL
                     GNQSQSDILALIIWLVGVGVYRLFVSIDLSIGTTLPSMAIVSLLYYTSTKGMMIWKER
                     KSQSSTT"
     gene            2658..3356
                     /locus_tag="VFD79_05870"
     CDS             2658..3356
                     /locus_tag="VFD79_05870"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF014198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HZH93612.1"
                     /translation="MERKKIPVLHHITNEVASNLQAQVTELMGLMPIMGNSSLDAHEI
                     ASMSSGFVVNLGMPTPDKLMAIRKTIPIYEMTGRPIVLDPVGISLSGTRRHLASELIK
                     GKGISVIKGNLSEISTLLHGKPEEIFTLEYNTVLVTTGRTIRITQGKRHELVEMPASK
                     INKLPGTGCILGTIIGCLLLKDFSPFDASIISANIMSKIAEKVPPNLPYGRIQESLLT
                     ELEVLANENLSYYR"
     gene            3331..3927
                     /gene="thiE"
                     /locus_tag="VFD79_05875"
     CDS             3331..3927
                     /gene="thiE"
                     /locus_tag="VFD79_05875"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HZH93613.1"
                     /translation="MKIYLITGDMKDESPWFEQIERILKSGIDMLQYRERQSSTLTMY
                     ERARKLKFLCKKYNTPLIINNRVDICMALDADGVHLGKDDLPLEEARKILGPKKIIGA
                     SARTIHAIRNGEINGVNYFGSGAIFETNTKKDAERISKDQLRLLAKSTSLPIYAIGGI
                     NEENVKELADTGIAGVCLSAGLMLNPYPERLLEIIKNI"
     gene            complement(3993..6062)
                     /locus_tag="VFD79_05880"
     CDS             complement(3993..6062)
                     /locus_tag="VFD79_05880"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZH93614.1"
                     /translation="MDKDKDRNIENDENPVKDEKMEQDKNHGKHRDHEDHNDHGGHDH
                     GDMVKDFKRRFFISLVLTLPILVLSPMIQGFLGVDWRFSGDMYILFGLSTVVFFYGGW
                     PFITGARDELEDRSPGMMMLIALAITIAYGYSSLVVFGWEGKQLFWELATLVDIMLLG
                     HWIEMKSVMGASNALEELVKLMPNEAHRLDEDGNAQDVPLSELVSGDRVLVKPGEKIP
                     VDGIIVEGKSAVDESMLTGESVPVEKKEDDEVIGGSVNKEGSLTVQVEKTGEDSYLSQ
                     VITMVREAQETKSKTQDLTNRAAKWLFYLALSAGFITLFVWLSLGYEFDMALERMVTV
                     MVITCPHALGLASPLVVAVSTTLSAKQGLLIRNRANFEGARNLDAVVFDKTGTLTKGE
                     FGVTDNVPSEGHEEDELLKIAASLEQNSEHPIATGIVNEAKERELELGKAEDFKALTG
                     KGIEGKVDGVEVKVVSIGFIENKDISYDKDHFDRMSEEGKTVVFVLKEGKLIGMIGLA
                     DMVRETAKEAIETLKEMGVKSIMLTGDNQKVADWVAKQLGIDEVYAEVLPDDKADQIK
                     RIKEDKGWKVAMTGDGVNDAPALATADLGIAIGAGTDVAMETADVVLVKSNPNDVVAL
                     MDLSRKTYRKMVQNLWWASGYNIIAIPLAAGVLAPWGIVLSPAVGAILMSMSTVIVAI
                     NAKMLKA"
     gene            complement(6072..6440)
                     /locus_tag="VFD79_05885"
     CDS             complement(6072..6440)
                     /locus_tag="VFD79_05885"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HZH93615.1"
                     /translation="MNTHKEFIDQIDELDKRDRVRMVLDHIKERYPQLKEEIKWNQPM
                     FSDHGTYIIGFSISKGHISLSPEAHTLDIFDEEIKKSGYERTKMTMKIKWKEKVDLDL
                     IDGMIDYNIEDKKDMTRFWR"
     gene            complement(6571..>7013)
                     /locus_tag="VFD79_05890"
     CDS             complement(6571..>7013)
                     /locus_tag="VFD79_05890"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZH93616.1"
                     /translation="ELVMARGTNGVLACQGDEGYPYAVPVSYVYWNDRIYFHSAKSGH
                     KVDAITRNPKVSFAVVDEDTIVSEEYTTYFRSVIAFGKARIVEGDEWFEGFKALVDKY
                     SGDLPEDANIKEIQGCRTSHIIAIDIDHITGKEAIEYVRAKQGK"
BASE COUNT         1905 a         1674 c         1466 g         1968 t
ORIGIN      
        1 ttctcaagtt aatagacaat tgtactaggg gagctgggat ggccagctga aagtgaaacc
       61 cgtaagtttc ttaccctcat acctgatctg gataatacca gcgtaggaaa gtaactactg
      121 catgatgtgc atattccaac gcaaggggat atgcattttt aattccttgc agacacattg
      181 cctcctagta caaaaataca aggaggtttt cttatgcaaa caaagaggct cgttttaagt
      241 ggattacttg tatcatttgg tgtacttgca agccatgtga tagtggttcc ggtcggtgtt
      301 gcaaaggcat atcctttgca gcacacaatt aatgttattg gagctgtgat gctgggaccc
      361 ggttatgctg ttgccaatgc attactgatt tctcttttga gaaacatttt gggagtcggg
      421 tcagtacttg catttccagg tagcatattt ggagcaatgc ttgcaggtct cttttacaag
      481 cattttaaaa atgaattttc cgctggcata ggtgaagtga ttggtacagg gattgttgga
      541 agtctggttg catttcccct gtcacgattc cttctggggt cagaaactgg tgctttcttc
      601 tttatccccc cttttctgat ttcaagcctc gtcggagcag ttcttggaat cttcacagtc
      661 aaatcactta gtggtcttgg aaatggggtg ttgttgaaat gaaaaaagtc ttaacaatag
      721 ctggtagtga ttcttcagga ggagcgggta tacagggaga tttaaaggca ttttctgcaa
      781 atgaaacctt tgggatgagt gttattactg cggtgactgc acaaaacaca cagggagttt
      841 acatggtcca cgacatccca aaggaagtga tccaagcaca gataaaggct ttattcaatg
      901 atgttgcaat cgatggaatc aaaattggca tggtatccat tcctgacacc atagaaatga
      961 tagccagaac cctaagcctt tattgtttgc ccaagattgt actggatcct gtaatggtct
     1021 caaagagtgg ttcaaatctc ctgcagccag ctgcagtaaa tgccctaaaa gagcagctga
     1081 ttcccatggc attcctcata acaccaaata tccctgaagc ggaagttctg accggcataa
     1141 agatattaag tgaagatgac atgatcatgg cagccagaga gcttatgaat cttggatgtg
     1201 ggggcgtcct cctaaagggt ggacaccttg atggtcctgc agttgatatt ctccatgacg
     1261 gtaaggaaat aacaagattc gaagctgata ggatttcttc caacaacaac cacggcacag
     1321 gctgcgccct atcttcgaca atttgttcca accttgccaa gggttacagc ctaccggtag
     1381 cagttgaaaa agggaagtcc tatgtatacg aaagcataag caatggcttt tcagtgggaa
     1441 aaggcgtcgg ggcccttcat cactttcata gtttctatga aggaggcgac cataatgaat
     1501 aaattcagcg gatttaacct tacggccctc tggtttggag ctgcaatttc agtagcggaa
     1561 ataatcaccg gaggcctgct ggcacctttg ggttttaagc ttggtctgct aataatcata
     1621 gtcgggcatg taatcggtgg tcttataatg gtggctgcag gcaacatagg atatgaaatg
     1681 ggacttgcaa gcatggaatc cactgaactt acctttggaa ggtacggaaa gaccctgttc
     1741 tccctactta atgcaaccca gcttgttgga tggacagtca tcatgcttat ttcaggcggt
     1801 gctgcaacat cagtgcttac ccggaatata ttctcctttg gtggtacttc aacctggata
     1861 attgttcttg gcttacttgt agccctttgg ctgtatgctg gtaaagggtt atggaaaacc
     1921 ggaaatgcca taatagggat ctcaatgctt gctttaacaa ttttcattat aaggattggc
     1981 cttgccaatg aaggtataac atcattgcct gacacaaata ccatatccat aggtctggca
     2041 attgaactga caatagtaat gccaatctca tgggtaccac ttgtggcaga ctacacaagg
     2101 gatagcaaaa gcaaaattgg gggtaattta ggtacattta tcggatattt ttttggtagc
     2161 tcacttatgt actttgtagg tctaattgca gcattgggat ttggcagcac ggacataggc
     2221 tccatactcc ttaatcaggg ctacggactt gttggcgttc tgataataat gctctccacc
     2281 atctccacaa cctatctgga tgccttcagt gcaggacttt ccattaaaaa tcttgtaccc
     2341 accctgtctg agaagcagct tggaacatgg gtcgttgcta tctcaacatt catagcatta
     2401 acctttccaa tgacggcata tgaaagcttt ctcctctgga taggcgcctt gtttgtaccc
     2461 ctgtttgcca tcctgattac cgattactac atcctgggta atcaatccca atctgacatc
     2521 cttgccctga tcatttggct tgttggggtt ggtgtataca ggttgtttgt aagcatagac
     2581 ctttccattg gcaccacgtt gccgtcaatg gccatagttt cactactata ctacacttca
     2641 acgaaaggaa tgatgatatg gaaagaaaga aaatcccagt cctccaccac ataacaaatg
     2701 aagtcgcctc taaccttcag gcacaggtaa cagagcttat gggactgatg cccataatgg
     2761 gtaacagcag tctggatgcc catgaaatag cctcaatgtc ttctggcttt gttgtgaacc
     2821 tgggaatgcc aactccagac aagcttatgg cgataagaaa aaccattcca atttatgaaa
     2881 tgaccggcag gccaatcgtc cttgatccag ttggaatttc attatcaggt accagaagac
     2941 accttgcaag tgagctgatt aaaggcaaag gaatttctgt cataaaagga aatctgagcg
     3001 agataagcac tcttctccat ggaaaaccag aagaaatttt cacccttgag tacaatactg
     3061 tgctagtgac tacgggtagg acaatccgaa taacgcaggg aaaaagacat gagttggtgg
     3121 aaatgccagc aagcaaaata aacaagcttc ctggaacagg ttgcatactg ggtaccatca
     3181 taggctgtct tttgttgaag gatttttctc cttttgatgc atcaattatt tcggcaaata
     3241 taatgtcaaa aattgcagaa aaagttccac ctaatcttcc atatgggagg attcaggaaa
     3301 gcttactaac agagttggag gttttggcca atgaaaattt atcttattac aggtgatatg
     3361 aaggatgaga gtccatggtt tgaacaaatt gaaagaattc tcaagtctgg gatagatatg
     3421 ctccagtaca gggagaggca aagctcaacc ctgaccatgt atgaaagggc caggaagctg
     3481 aaatttcttt gcaaaaaata caatacaccg ctgatcataa acaacagggt tgatatctgt
     3541 atggcattgg atgcagacgg tgtgcatctt ggcaaggacg acctacccct tgaagaagca
     3601 cgaaaaatcc tgggtccaaa gaagataatc ggtgcgtcag caagaaccat ccacgccatc
     3661 aggaacgggg aaatcaacgg ggtcaattat tttgggtcag gggcaatctt tgaaacaaat
     3721 acaaaaaaag atgctgaaag aataagcaag gatcaactca gacttcttgc caaaagcacc
     3781 tccctcccaa tatatgccat aggtggaata aatgaggaaa atgtaaaaga gctcgcagat
     3841 actggaattg caggagtttg tctgtcggcg ggccttatgc tcaatccata ccctgaaaga
     3901 ctactggaga taatcaaaaa tatttgaaaa agaaaaatac cctttgaaag gatttgtgta
     3961 tttacaatcc attccaaagg gtatttctga agctatgcct tcagcatctt ggcgttaatt
     4021 gcaacaataa ccgtactcat gctcatcagt atggctccaa ccgcagggct aaggactatc
     4081 ccccagggag ccagcactcc tgcggcaagg ggtattgcga ttatattgta tccacttgcc
     4141 caccaaaggt tctggaccat cttcctatag gtcttcctgg aaaggtccat tagggcaacc
     4201 acatcattgg ggttgctctt aacaagaaca acatcagcag tctccatggc cacatctgtt
     4261 cctgcgccaa tggcaatccc aaggtctgcc gttgcaagag cgggtgcatc gtttacacca
     4321 tcacctgtca ttgcaacctt ccaccccttg tcctctttaa tccttttgat ctggtcagcc
     4381 ttgtcatcag gaaggacttc ggcatagacc tcatcaattc ccagctgttt ggctacccag
     4441 tcagcaacct tctggttgtc ccctgtaagc ataatggact tgactcccat ttccttaagg
     4501 gtttcaatgg cctccttggc ggtttcccta accatatcgg caagaccgat cattccaatg
     4561 agcttaccct ccttaaggac aaagacaacg gtcttacctt cctcgctcat cctgtcaaag
     4621 tggtccttgt cataagaaat atccttgttc tcaataaaac ctatgcttac aaccttgacc
     4681 tccactccat cgacctttcc ctcaattccc tttccggtca aggccttaaa gtcttctgcc
     4741 tttccaagct caagctccct ttcctttgcc tcatttacaa tccctgttgc gattgggtgc
     4801 tctgagttct gctcaaggct tgctgcaatt tttagaagct catcctcctc atgcccttca
     4861 gaaggtacat tatcagtaac tccaaattcc ccttttgtaa gggtgcctgt cttgtcaaac
     4921 acaactgcgt caaggtttct cgcaccttca aagttggccc tgttccttat aagaagtccc
     4981 tgttttgcag aaagtgtggt tgaaacagca acaacgagtg gggatgccag acccagcgca
     5041 tggggacaag tgattaccat tactgtaacc atccgctcaa gggccatatc aaactcatag
     5101 ccaagggaca gccaaacaaa cagggtaata aatcctgcac tcagggccaa atagaagagc
     5161 cactttgcag ccctgtttgt aaggtcctgt gtctttgact tggtctcctg ggcttcccgg
     5221 accattgtga taacctgtga aaggtaggaa tcctccccag tcttctcaac ctggactgtc
     5281 agtgagccct ccttgttcac agacccacct atgacctcat catcctcctt cttctcaacg
     5341 ggtacagact ctcccgtaag cattgattca tcaactgctg acttaccctc aacaattatt
     5401 ccgtcgacgg gtatcttttc accaggctta accagaaccc tgtctccact tacaagctcc
     5461 gacaggggca catcctgggc attgccatct tcgtccaatc tatgggcctc gtttggcata
     5521 agcttgacca gctcctccag ggcattggat gcacccataa ctgatttcat ctcgatccag
     5581 tgaccaagca gcatgatgtc aacaagggtt gccagctccc agaacagctg ctttccttcc
     5641 cagccaaata caacaaggct gctgtagccg taggcgatgg tgatcgccag ggcgataagc
     5701 atcatcattc caggactcct gtcctccagc tcatccctgg ctccagttat gaagggccat
     5761 ccaccgtaga agaataccac agtggacaat ccaaagagga tgtacatgtc cccagaaaat
     5821 ctccagtcaa ctccaagaaa accctggatc attggagaca atacaaggat tggaagggtc
     5881 aggaccaggg agataaagaa ccgtctcttg aaatccttca ccatatcccc atggtcatga
     5941 cctccatggt cattatggtc ttcatggtcc ctgtgcttgc catgattttt gtcttgctcc
     6001 attttttcat ccttcacagg attctcatca ttttcaatat tcctatcctt gtccttatcc
     6061 atatcaaata cctacctcca aaatcttgtc atatccttct tgtcctcaat attatagtca
     6121 atcattccgt ctatcaggtc aaggtccacc ttctccttcc acttgatctt catggtcatc
     6181 tttgtcctct catagcctga ttttttgatc tcctcatcaa aaatatccag ggtatgggct
     6241 tccggtgaaa gtgaaatgtg ccccttggat atgctaaagc caatgatata ggttccgtgg
     6301 tcactgaaca tgggctgatt ccactttatc tcctccttca gttgaggata cctctccttt
     6361 atatggtcaa ggaccatcct taccctgtcc cttttatcaa gctcatctat ctggtctata
     6421 aattccttgt gagtgttcat cctaaatccc tcctttatgc tttgataccc gtaacgaccg
     6481 gtcattctaa catataccta ttaaagacat atgctcacat attatctttg gacaaagaaa
     6541 aaagcgggct tccccgcttg ataattgaat ctattttccc tgtttggccc tgacatactc
     6601 aattgcttcc ttaccggtga tatggtcaat gtctatggca attatatgtg aagtcctgca
     6661 gccctggatc tcctttatgt tggcatcctc gggaaggtct cctgaatact tgtccaccag
     6721 ggccttaaag ccctcaaacc actcatctcc ctctacaatc ctggcctttc caaatgcaat
     6781 gacacttcta aaatatgtag tgtactcctc gcttacgatt gtatcctcat caacaacggc
     6841 aaaggatacc tttggattcc ttgttatggc atccaccttg tggccagatt ttgctgagtg
     6901 gaaatatatc ctgtcattcc agtatacata gcttacggga actgcatagg gataaccctc
     6961 atcaccctgg caggcaagta ctccatttgt tcccctagcc attacaagct cca
//
LOCUS       DASRQF010000221         2378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1810128, whole genome shotgun sequence.
ACCESSION   DASRQF010000221 DASRQF010000000
VERSION     DASRQF010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1810128"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VFD79_05895"
     CDS             complement(<1..265)
                     /locus_tag="VFD79_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019115748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HZH93617.1"
                     /translation="MEKEVYNVYSTYLKDKYGEKVYKLPVKLPLTCPNRDGNLSKGGC
                     TFCGEEGGSFENLSSCMSVGDQLSINMDHIGKKYKAKKFIAYFQ"
     gene            complement(281..1201)
                     /locus_tag="VFD79_05900"
     CDS             complement(281..1201)
                     /locus_tag="VFD79_05900"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZH93618.1"
                     /translation="MKTRKTKNNPIWKYLAAARYFLFKYEVRFLYRLIAIDLDGTLLN
                     SSKRISEENRDAVNRLIGLGYQVVIATGRRYYSAKMLVKDLDSHLVIMANNGNIVRSS
                     LDDVVLISKFLDRGSYLDILKEAKDHKLRGIVHIDRYLEGIDMVVEKDDQEGEHMKYL
                     TEQDKRYLVLPERDFYQLDRILAVVFPGDYKAVKTFSDMIGQKYPDRYSSHVLENIYI
                     AEAMYEAMNPGGSKWKTLHEYAESIGVNRDEIIAIGDDNNDVDMILNAGLGIAMKNGS
                     GLAKAAADIVTEWDNDHSGLARVLGKVLNF"
     gene            complement(1176..1520)
                     /locus_tag="VFD79_05905"
     CDS             complement(1176..1520)
                     /locus_tag="VFD79_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HZH93619.1"
                     /translation="MSDNKHVHDENCNHDHDHDHEEMEIITLTLDDDSELECAVIGIF
                     EVEDKEYIALLPLGDEEEEEEDEVLLYEYKDLGEEEFELALIEDEEEFDLVSEAFYAL
                     YTDEDEDEEDEE"
     gene            complement(1590..>2378)
                     /locus_tag="VFD79_05910"
     CDS             complement(1590..>2378)
                     /locus_tag="VFD79_05910"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZH93620.1"
                     /translation="DKGRGNSNRLLARVLTGMKGIDYELLHIEGGSKSNAIPRLSIAR
                     LSMDDDNLHKAKGIVESYQEAFRKELATTDEGLQVSLAEIDHDGKAMTRETRDRIIGA
                     MMLIPTGVQSMSQDIEGLVESSNNMGVVTTHEDRVTIENAIRSSVKTLKEYIAAQIGS
                     MADGLGLQWETSGDYPAWEYRKDSYIRDVFIQAHKEITGKDPKVEAIHAGLECGLFDE
                     VMEDVDMISLGPNMAGVHAPGERLSISSTERTYKLLLEALKKIN"
BASE COUNT          537 a          642 c          467 g          732 t
ORIGIN      
        1 tctgaaaata tgctatgaat ttttttgcct tgtacttttt acctatatgg tccatgttaa
       61 tggacagctg gtcccctacg gacatgcatg aagaaaggtt ttcaaaagac cctccctctt
      121 caccgcagaa ggtacatcct cccttgctca ggttgccgtc acggttggga caggttaggg
      181 ggagttttac ggggagcttg taaaccttct cgccatattt gtcctttaga taggttgaat
      241 atacgttgta aacttccttt tccacattac acctctgctt ctaaaaattt aaaaccttcc
      301 caagaacacg ggcaaggcct gaatggtcat tgtcccattc agttacaata tctgcagctg
      361 ccttggccag tccgcttcca ttcttcattg caattccaag tccagcattt aggatcatgt
      421 ccacatcgtt gttgtcatct ccaattgcaa ttatttcatc ccggttcaca ccaatggatt
      481 cagcatactc atggagggtc ttccacttgc ttccccctgg gttcatggcc tcatacatgg
      541 cctctgcaat gtagatgttc tcaagaacat gtgagctata cctgtccggg tacttctgac
      601 cgatcatatc gctgaaggtt ttcacggcct tgtagtcacc tggaaacacc actgccagga
      661 tcctgtcaag ctgatagaaa tctctttcag gcaggacgag atacctcttg tcctgctcgg
      721 tgagatactt catgtgttcc ccttcctggt catccttctc caccaccatg tcaatcccct
      781 caaggtacct gtcaatgtgg actattcccc gaagcttgtg gtccttggcc tccttgagaa
      841 tgtccaggta agatcccctg tcaaggaact ttgatatgag cacaacatca tccagggagc
      901 tcctaacgat attcccgttg tttgccatta tcacaaggtg gctgtccagg tccttgacca
      961 gcattttggc ggagtagtac cttcttcccg ttgcaatcac aacctggtat cccaacccaa
     1021 taagccgatt gacagcatcc ctgttctcct cagaaatccg cttgctgctg ttaagcaatg
     1081 tcccatcaag gtctatggca ataagtctat acaaaaatct cacctcatat ttgaaaagaa
     1141 agtaccttgc agctgcaagg tacttccaga ttggattatt cttcgtcttc ctcgtcttca
     1201 tcctcgtcag tgtaaagggc atagaatgct tctgacacaa gatcaaactc ctcttcatcc
     1261 tcaatcaatg caagttcgaa ttcctcttca ccaaggtcct tgtattcgta cagaagaacc
     1321 tcgtcctcct cttcctcttc ttcatcacca agtggtagaa gggcgatgta ctccttgtcc
     1381 tccacttcga atattcctat tactgcacat tccagctcag aatcatcatc tagtgtaagg
     1441 gttataatct ccatttcctc atggtcatga tcgtggtcat ggttgcaatt ttcgtcgtgc
     1501 acgtgtttgt tatcgctcat catcattctc cttttcttat attctactgt aatagtacct
     1561 tatttggggt accttataca agtgaaaact taatttattt ttttcagggc ttctagaagc
     1621 agcttgtagg tcctctcagt ggatgaaatg ctgagtcttt ccccaggtgc atgaacccca
     1681 gccatgttgg gcccaaggga aatcatgtcc acatcctcca tgacctcgtc aaaaagaccg
     1741 cactcaaggc ctgcatgtat tgcctcaacc ttgggatcct tgcctgtaat ctccttgtgg
     1801 gcctggatga agacatccct gatgtaggag tccttcctgt actcccatgc aggatagtct
     1861 ccagatgttt cccattgaag tcccaaaccg tcagccatgc ttccaatctg ggctgctata
     1921 tactccttca gggtcttcac tgagcttctt attgcattct caatggtgac cctatcctca
     1981 tgggtggtta caacacccat gttgtttgag ctctccacaa gtccttcaat atcctggctc
     2041 atggactgga cacctgttgg tattagcatc atggccccta ttatcctgtc cctggtctcc
     2101 ctggtcattg ccttgccgtc atgatctatt tcagccaggg atacctggag tccctcatcg
     2161 gtggttgcga gctccttcct gaaggcctcc tggtagctct cgactatccc tttggctttg
     2221 tgaagattat catcatccat ggaaagcctt gcgatggaaa gccttggaat ggcatttgac
     2281 ttggatcctc cctcaatatg gagaagctca tagtcaatgc ctttcattcc tgtcagaacc
     2341 ctggcaagta gtctgttgga attgcctctt cccttgtc
//
LOCUS       DASRQF010000222         4325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_978709, whole genome shotgun sequence.
ACCESSION   DASRQF010000222 DASRQF010000000
VERSION     DASRQF010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978709"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VFD79_05915"
     CDS             <1..271
                     /locus_tag="VFD79_05915"
                     /inference="COORDINATES: protein motif:HMM:NF020581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FeoC-like transcriptional regulator"
                     /protein_id="HZH93621.1"
                     /translation="GGYEMLKEVLKEIKQANVYSKPQIAKNLDVSENVLEDMIKTLVR
                     MGYLEEDLGSPTCETKCSGCSVSSCNTIPIKMLSITEKGDRKINL"
     gene            1154..2608
                     /locus_tag="VFD79_05920"
     CDS             1154..2608
                     /locus_tag="VFD79_05920"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HZH93622.1"
                     /translation="MDKFDWTDKKNLTMLADYYEFTMANGYLQNGMHDTIAYFDMFFR
                     SIPDDGGYAIMAGVEQLIDYLKTLSFDKEDIEYFRSKDIFSEEFLDYLRNFKFVCDVW
                     AIPEGTPIFPREPLVVVRGPVIQAQLVETMILLTINHQSLIATKASRIVRAADGRSVI
                     EFGSRRAQGYNGAIQGARAAYIGGCIGTSSTIVDRDFGIPALGTMAHSWVQMFPDELE
                     AFRAYARAYPGSCTLLVDTYNTLKTGIPNAIKVFDEEVVSRGFRPKGIRIDSGDIAYL
                     SKEARAMLDEAGYEDVQIMASSSLDEFVIKDLVTQGAKIDAFGVGERLITAKSEPIFG
                     GVYKLVAIEENGVMKPKIKISNNVEKITTPGFKQIYRFYDKKTHTPMADVITLHDEKV
                     PEDGPYEIFHPLFTWKRKTLTDYYTRPLLVQLFHKGRSVYESPDVHEIRDFCRSEIEN
                     LWDEVKRFERPHEYFVDLSQDLWNIKDKMLISHK"
     gene            complement(2634..3269)
                     /locus_tag="VFD79_05925"
     CDS             complement(2634..3269)
                     /locus_tag="VFD79_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HZH93623.1"
                     /translation="MAVYKMTPTKANLIKSKNSLRFALKGFELLDKKRVVLIREMMAL
                     IDVAEDLQWRIDENFAQAYEALKLANITKGSEYIEDFAQSIEKERDYQVLFKSVMGVE
                     VPIIKREEKSLTTEYGFFRTNAAFDKTVLSFIEIRNLIYQLAEVENSVYKLAMEIKKT
                     QKRANALDKIQIPRYKTTIKYIDEVLQEKEREDFFRLKRVKGKTKSKKTTK"
     gene            complement(3279..>4325)
                     /locus_tag="VFD79_05930"
     CDS             complement(3279..>4325)
                     /locus_tag="VFD79_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004825443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HZH93624.1"
                     /translation="NINGRPINPVSREYPRNFIQTGISSIDGLTTLIRGQKLPIFSGA
                     GLPHNELAAQIVRQAKIQSKDGDSNFSIVFAAIGVKHDEAFYFEDAFRKAGVQEKVVM
                     YINYADDPIMERITAPKCALAAAEYLAYEENQHVLVIMTDITSYGEALREVSSLREEV
                     PSRKGYPGYLYSDLASLYERAGMIHGINGSVTLIPILTMPNDDITHPIPDLTGYITEG
                     QVVLERSLDQKGIYPPINVLPSLSRLMKDGIGEGFTREDHPEVSNQLFSSYSKVQEVR
                     ALAQIVGEEDLSEKDKYYMKFGDAFEGRFVTQSLNENRDIEDTLDLAWELLKILPREE
                     LDRLNPELIAKYMG"
BASE COUNT         1288 a          876 c          908 g         1253 t
ORIGIN      
        1 aggagggtat gaaatgctaa aggaagtact aaaggaaata aagcaagcaa atgtatattc
       61 aaaaccacaa atagccaaga acctggacgt atctgaaaat gtacttgagg atatgataaa
      121 aaccctggtg agaatgggat accttgagga ggaccttgga tcacccacct gtgagaccaa
      181 atgctcggga tgttcggttt caagctgcaa cacaattccc ataaagatgc taagcataac
      241 cgaaaaggga gatcgaaaaa ttaatttata aaaattttat atatttaaat aagaagaagg
      301 agccatttaa aggacttcct tcttttttta tataaacttt tttgtttttt catgtttaaa
      361 agaatatatg tgggtataat ataactacaa atcatatatt aactcactgc ttcaatgaag
      421 caaagttgat attatggttt catgggactt tgataactgc atccaactaa aattcaatca
      481 ataagttgta gaagtttatt gaacctggaa agattcaagt aacaatgcaa agtattgata
      541 tggattcagg taaaatctga attcagaagc tacacctatt tagaagttac tgttgtagaa
      601 gctaaatgaa acaagctgta ggaagttggc aatgtaagaa aagatagtac tgtagaacaa
      661 gtttaagatg tagaaagttt tagatgtaag ttgctacacc tattaaagta ttactgttgt
      721 aaaattccag gtttaagtag tttatgcaag tatgatggta aaagaaaagt agaaagtagt
      781 aaggtagtaa aaacgcagta aaggaagtac ctgtaacagg gatttgtggt atccccataa
      841 accacagaaa aagtaacctt agggttatga aagtgtaaca actaaataac ttattaggtt
      901 ataagattag agtttaatcc tctgtaactt aaattaagga gtgtttaagg ataagtgatt
      961 gaaccaaaaa gttcatgttg gagcagttat tcaaggtcct atttaatatg aaaggtttat
     1021 cccaatgaaa aaggatggac ctttcttatt tttttgttct tttttccaat atattgaaag
     1081 ttttcaatta aatctccaaa atacgctgaa aacttgctat aatgtacata acatagaaaa
     1141 aaggagagta accatggata aatttgattg gaccgacaag aaaaacctga ccatgctggc
     1201 agactactat gagttcacca tggcaaatgg ttatttacag aacgggatgc atgacaccat
     1261 tgcttatttc gacatgtttt tcagatccat acccgatgac ggagggtacg caatcatggc
     1321 tggggtcgag cagttgattg actacctgaa gaccttaagc tttgacaagg aggatattga
     1381 atattttaga agcaaggata ttttcagcga agaatttctg gactatttaa ggaacttcaa
     1441 attcgtatgt gatgtgtggg ccattccaga aggcacaccc attttcccaa gggagccctt
     1501 ggttgttgta aggggaccgg tgattcaggc ccagcttgtt gaaaccatga tcctactaac
     1561 cataaaccat caatccctga tagccaccaa ggccagcagg attgtacggg cagcagacgg
     1621 caggagcgta atcgagtttg gctccagaag ggcacaggga tacaacggtg ctatccaggg
     1681 ggccagggct gcatatatag gtggctgcat cggcacctcc agtacaattg tggataggga
     1741 ttttggtatt ccagcacttg gaacaatggc ccacagctgg gttcaaatgt ttccagacga
     1801 gctggaagcc tttagggcat acgcaagggc ctaccctgga agctgtaccc ttcttgtaga
     1861 tacctacaat acactaaaga ccggaatacc caatgccatt aaagtttttg acgaagaggt
     1921 tgtttcaagg ggattcagac ctaagggaat caggattgac agtggcgata ttgcttatct
     1981 ttccaaggag gccagagcaa tgcttgatga agcaggctac gaagatgttc aaataatggc
     2041 ctcaagcagc ctggatgaat ttgtaataaa ggatttggtc acccagggag caaagattga
     2101 tgcctttgga gttggggaga gacttattac tgcaaagtct gagccaattt tcggtggtgt
     2161 gtacaagctt gtggcaattg aagaaaacgg cgtgatgaag ccaaagataa agataagcaa
     2221 caacgttgaa aaaatcacaa cacctggatt caagcagatc taccggttct acgacaagaa
     2281 aacccacaca ccaatggctg atgttatcac tctccatgac gagaaggtgc ctgaggatgg
     2341 tccttatgaa atcttccacc cattgttcac atggaagcgc aagaccctta cagactacta
     2401 cacacggcct cttcttgtac agcttttcca taagggcaga agcgtctatg aaagtcctga
     2461 tgtccatgaa atcagggatt tctgcaggtc tgagattgag aatctgtggg atgaggtaaa
     2521 gagatttgaa agaccacacg aatactttgt ggacctgtca caggacctct ggaatatcaa
     2581 ggataaaatg cttataagcc acaaataagg aggaagccga aaggcttcct ccattatttt
     2641 gttgtctttt ttgattttgt ctttcccttg acccttttaa gtctgaagaa gtcttccctt
     2701 tccttctcct gaagtacttc gtcaatgtac ttgattgtgg ttttgtatct gggaatctga
     2761 atcttgtcaa gtgcattggc tcttttttgc gtcttcttaa tctccatggc taacttgtag
     2821 actgagtttt caacctctgc aagctggtag atcaggtttc tgatctctat aaagcttaga
     2881 accgtcttat cgaaggcagc attcgtcctg aagaatccat attctgtggt taagcttttc
     2941 tcttccctct tgattattgg gacctcaaca cccattacgg atttgaaaag cacctggtaa
     3001 tccctttcct tctcaatgga ttgggcgaaa tcctcaatat actccgatcc cttggttata
     3061 tttgccaact tcagggcttc gtaagcctga gcgaagtttt cgtcaatcct ccactgaagg
     3121 tcctctgcca cgtctatcaa agccatcatt tcccttatta gaactaccct tttcttgtcc
     3181 agcaactcaa agccttttag ggcaaatcta agtgaatttt tggatttgat caagtttgcc
     3241 ttggttggag tcatcttata tacagccata aactcaccct atcccatata ctttgctata
     3301 agttccggat tcaatctgtc caattcctct ctaggcagga ttttcaacag ctcccatgcc
     3361 agatccaatg tatcctcaat atccctgttc tcgttaagac tctgggttac aaaccttccc
     3421 tcaaaagcat ctccaaactt catgtagtac ttgtccttct cagaaaggtc ctcttcacct
     3481 actatctgtg caagagctct aacttcctgt acctttgagt atgatgagaa cagctggttg
     3541 gaaacctcag gatggtcctc cctggtaaag ccctccccta ttccgtcctt catcagacgc
     3601 gaaagtgaag gcaaaacatt tattggcggg tagataccct tttgatccaa tgatctctcc
     3661 aatacaacct gtccctcagt gatgtagcca gtaaggtcag gtattgggtg tgtgatatca
     3721 tcatttggca tggtaaggat tggtataagt gtaaccgatc cgttgatgcc gtgaatcatt
     3781 ccagcacgct cataaagtga tgccaggtct gagtacaggt atccaggata tccctttcta
     3841 cttggcacct cttccctcag tgatgatacc tctctcaagg cctcaccgta gctggttatg
     3901 tctgtcatta ttacaagaac atgctgattt tcctcataag ccaggtattc agcagctgca
     3961 agtgcacatt ttggagctgt tatcctctcc attattgggt cgtctgcata gtttatatac
     4021 ataacaacct tttcctgcac accggctttc ctaaatgcat cctcaaagta gaaggcctca
     4081 tcatgcttaa caccaattgc agcaaatact atggagaaat tgctgtcccc gtccttggac
     4141 tggatctttg cctggcgaac aatctgtgca gcaagctcat tgtgaggaag tcccgcacct
     4201 gagaatattg gcagcttctg ccctctgatc aatgttgtca acccatctat tgaagatatt
     4261 cctgtctgga taaagtttct agggtattcc ctggaaacag ggttgatcgg tctaccgtta
     4321 atgtt
//
LOCUS       DASRQF010000223         2662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_263663, whole genome shotgun sequence.
ACCESSION   DASRQF010000223 DASRQF010000000
VERSION     DASRQF010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263663"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /gene="ispG"
                     /locus_tag="VFD79_05935"
                     /gene_synonym="gcpE"
     CDS             complement(<1..904)
                     /gene="ispG"
                     /locus_tag="VFD79_05935"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HZH93625.1"
                     /translation="MNNRKKTRMIRVGDVYIGGDAPVSVQSMTNTPTSDVKRTLGQMR
                     ELQEAGCDIVRSAVSSAEDARAIREIKKHISIPIIADIQFDYRLAIESIRQGVDGLRI
                     NPGNIGSHLNVKEVVKACKSENISIRIGVNSGSVKREYLEKYNGVNEKSMVESALDQV
                     KLLEDLDFRDIKISLKASSVPLTVSSYALISEKCDYPLHLGVTEAGTPWRGTIKSSVG
                     IGALLAMGIGDTIRVSLTGDPVDEVKVGKEILKSLGLRNDGIEFISCPTCSRTHIDLI
                     GIAQEAERRLDHIKKPIKVAVMGCA"
     gene            complement(961..1965)
                     /gene="rseP"
                     /locus_tag="VFD79_05940"
     CDS             complement(961..1965)
                     /gene="rseP"
                     /locus_tag="VFD79_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HZH93626.1"
                     /translation="MITALAAIFVFLLVVLVHEAGHFTVAKMVGIKVNEFAIGMGPKL
                     IQRKKGETQYTLRALPIGGYVKMEGEDESSDDPRGFANVSVGSRIAVISAGAIMNFVL
                     AIVVLSLVAFNVGVPSTTIDEIIEGSPAQEYGIVQGDRVVQIEGNPINNWEDIVSTIN
                     SQDPETFIQVQVDRGGEIVNLDVKPTLDEGRVVIGIVPAYETGFFSAIKSGFQETWYF
                     LKLMFQFLGMLFQGNVSTNDLAGPVGVINEIGNAASMGFTSVLYILGFISINLGFFNL
                     LPIPALDGSKIVFLLIELLRGKPIDADKEGFIHFIGFVFLISLMLIVTYKDLIRINLF
                     "
     gene            complement(1985..>2662)
                     /locus_tag="VFD79_05945"
     CDS             complement(1985..>2662)
                     /locus_tag="VFD79_05945"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HZH93627.1"
                     /translation="LFLSLIALREFYNAVRKMGLNPFDIVGYSGAVLLFAANYFGIIS
                     IDFIISLVLVVSLAATVISKGRNIKDSAITMIGILYIPFLLFHILYLDGSVYLWLIFL
                     IAFGTDTFAYLIGSKYGKNKLCPEISPKKSVEGAIGGIIGSILVSLLYSMFTEISPLW
                     SVATLALAGSIISQLGDLTASRIKRAAGIKDYGRIMPGHGGMLDRFDSIIFAAPVVYY
                     YAHYFFM"
BASE COUNT          785 a          629 c          454 g          794 t
ORIGIN      
        1 ccgcacaccc catcacagca acctttattg gttttttgat gtggtccaat ctcctttcag
       61 cctcctgggc aatccctatc aggtcaatgt gagtacggct acaggtcgga caagaaataa
      121 actcaatccc gtcattcctc aaaccaagag atttgagaat ctcctttcct accttgacct
      181 catcgaccgg atcaccggtc aatgataccc tgatggtgtc cccaattccc attgccagaa
      241 gagcacctat tcctacagag gactttatgg ttcccctcca aggtgtgcca gcttctgtta
      301 caccaagatg caatgggtag tcacattttt cagatatcaa agcataggaa cttacggtca
      361 gtggtacaga gcttgccttt agggatatct tgatatctct aaaatccagg tcctccagca
      421 gtttgacctg gtccagggca ctttccacca ttgacttttc attgacacca ttatattttt
      481 caagatactc ccttttaaca gacccagaat tcacacctat tctgattgaa atattctcag
      541 atttgcaggc cttgacaact tccttgacat tcaggtgact gcctatgttt cctggattaa
      601 ttcgaagacc atcaacacct tgtctgatgg actcaatggc tagtctatag tcaaactgga
      661 tatctgctat tattggtatg cttatatgct ttttgatttc cctgattgcc cttgcgtcct
      721 ctgctgatga tacagcagac cttacaatat cacaaccagc ttcctgtaat tccctcattt
      781 gtcccaaggt tcttttcaca tcagatgtag gggtatttgt catggactgt acactaaccg
      841 gcgcatcccc acctatatat acatctccta ccctgatcat tcttgttttc ttcctattat
      901 tcatataatc caccttatac tccattttaa aatattatcc atcagcggtt aaaatcaaaa
      961 ctaaaataga ttgatcctga taagatcctt gtaggttact atcagcataa gggaaataag
     1021 gaatacaaat cctataaaat ggatgaatcc ctccttatct gcgtcaattg gctttccacg
     1081 taggagctcg atcaggagaa atacgatctt gctaccatcc agtgcgggta ttggtaacag
     1141 gttaaagaat cccaggttaa tactgataaa tccaaggata taaagaacac ttgtaaagcc
     1201 catactggca gcattcccta tttcattgat aacaccaaca ggtcctgcca gatcatttgt
     1261 ggaaacattt ccctgaaata gcatacctag gaattgaaac ataagtttta agaaatacca
     1321 tgtttcctga aacccggatt ttatagctga gaaaaatcca gtttcatatg ctggaacaat
     1381 tcctattaca accctgccct catcaagtgt aggcttaaca tcaaggttta caatttcgcc
     1441 gcctctatcc acctggacct gaataaaggt ctcaggatcc tgagaattta ttgtagaaac
     1501 tatatcttcc caattattta ttgggttacc ttcaatctga acaactcgat ctccttgcac
     1561 aataccatat tcctgtgctg ggctaccttc aatgatctcg tcaatggtag ttgaaggaac
     1621 ccctacatta aaggccacta gtgataatac aacaatggcc aggacaaagt tcattattgc
     1681 accggctgag attacagcga ttctggaacc tactgagacg ttggcaaagc ctcttggatc
     1741 gtctgaggat tcatcctctc cttccatctt tacataacca cctatgggta aggcccttaa
     1801 ggtgtactgt gtctcgccct tctttctttg aataagcttg gggcccattc ctatggcgaa
     1861 ttcgttgact ttgattccaa ccatcttcgc tactgtaaaa tggccggcct catgaacaag
     1921 gacaaccagt aaaaatacaa atattgcagc taatgctgtt atcatttatt tcacctctgt
     1981 ttttctacat aaaaaagtaa tgtgcataat agtagacgac tggagccgca aatattatgc
     2041 tgtcaaatct atccaacata cccccatgtc ctggcattat tctcccatag tcctttatac
     2101 ctgctgctct tttgattctg gaagcagtaa ggtcgcccaa ttgtgaaatg attgaaccgg
     2161 caagggcaag cgttgcaaca ctccatagtg gtgatatttc agtgaacatg gaataaagta
     2221 atgaaacaag tatgctccca attattccac ctattgctcc ctcaactgat ttttttggac
     2281 tgatttcagg gcaaagcttg tttttcccat acttgctccc aataagatag gcaaatgtgt
     2341 ctgtgccaaa tgcaatcagg aaaatcaacc atagataaac acttccatca agatataaaa
     2401 tatggaaaag caggaatggt atgtacagaa tcccaatcat tgtaattgcg gaatccttta
     2461 tgtttcttcc cttggaaatt acagttgctg ccaatgatac caccaatacc agagatataa
     2521 taaaatcaat agaaataata ccgaaataat tagctgcaaa caataatacc gcacctgaat
     2581 acccaactat gtcaaatggg ttaagaccca tttttctaac tgcattgtag aattctctta
     2641 aggctatcaa tgataagaac aa
//
LOCUS       DASRQF010000224         3761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1066320, whole genome shotgun sequence.
ACCESSION   DASRQF010000224 DASRQF010000000
VERSION     DASRQF010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066320"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="VFD79_05950"
     CDS             <1..828
                     /locus_tag="VFD79_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HZH93628.1"
                     /translation="RRRELYRNMLAVGKLDLVLAKAKLAIDMDATKPVILDDHIISIK
                     RGRHPKIEDLLRDRDLEFTPISIDLKEGVACITGANMGGKTISLKLVGLLSAMAHYGL
                     YVPAEEMELGLSLYIKSSIGDMQSTDSGLSTFGGEVQVVSDAMERADEKGLILIDELA
                     RGTNPEEGYAISKAIAIYLMDKPAISLITTHYDNVADIEGIQHLQVVGLSELDVDTLS
                     EEISEGDGMEIINKYMDYRLRVVDKTTGIPRDAINIARIMGLRTEILKIAEEILVKE"
     gene            871..1290
                     /locus_tag="VFD79_05955"
     CDS             871..1290
                     /locus_tag="VFD79_05955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HZH93629.1"
                     /translation="MNDISENVIEKKSYAFAINVVNAYKKLESRSEYVLSKQLLRSGT
                     NIGANVMEGLRAQSRKDFISKMNIALKEANESEYWIRLLMDTGYLSKQEDKSLLDECK
                     EVCRILSSIVRTANNNLDNKVKETTEEYITSIIEEAN"
     gene            1456..3009
                     /locus_tag="VFD79_05960"
     CDS             1456..3009
                     /locus_tag="VFD79_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HZH93630.1"
                     /translation="MSKLNLDLKKIEASRASARNIAEDIQNFIDEHTTVSTERTVVRL
                     LGVDGVDEIGKPLPNVVVDNIVEGGGLERGAAYWIGNAMLNLKIPPQEIAEKIATGEV
                     DITRLPARDDQEIRDVIHTVAEETVEKILANKKVRDDYIADFGPAKTPNLYVIVATGN
                     IYEDIVQAKAAAKQGADIIAVIRTTGQSLLDYVPYGATTEGFGGTYATQENFKLMREA
                     LDEVGEEIGRYIKLTNYCSGLCMPEIAAMGALERVDMMLNDALYGILFRDINMQRTLV
                     DQYFSRVINGFAGIIINTGEDNYLTTDDAVEAAHTVLASQFINEQFALKAGLPEEQMG
                     LGHAFEMEPTLEDGFLLELAQAQMAREIFPKAPLKYMPPTKFMTGNIYKGHVQNAMFN
                     MVSIMTGQGIQLLGMLTEAIHTPHLHDRYLSIENAQYVFNNARHLSDEIEFKKDGVIQ
                     KRAHAVLEEASDLLQQIEREGLFQTIEQGKFGGVRRMRTGGKGLAGIARKDSGYFNPF
                     IQLMLGGDR"
     gene            3009..>3761
                     /locus_tag="VFD79_05965"
     CDS             3009..>3761
                     /locus_tag="VFD79_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009431793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HZH93631.1"
                     /translation="MSDIQKVDLTKVKPYGDTMNDGAVQLSFSLPVPYGDEANEAARQ
                     LAKKMGFEEPAVVYSHDLGIDYTYFVVYGKTIHTVDYSSIEVPKVDVDVMEKHDIEDY
                     IKDNIKRDVVILGACTGTDAHTVGIDAIMNMKGYAGHYGLERYEGIQAYNLGSQVPNE
                     ELVAKAIEMKADALLVSQVVTQKDVHIPNLTELVELLEAEGIRDKIVLVCGGPRLSHE
                     LAKELGYDAGFGSGSYAEDVATFVVNEIVKRNL"
BASE COUNT         1206 a          775 c          962 g          818 t
ORIGIN      
        1 aggagacggg agctctacag aaacatgctg gctgttggaa agctggacct ggttcttgcc
       61 aaggccaagc ttgcaataga tatggatgca acaaagcctg tgatccttga tgaccacatc
      121 ataagcataa aaaggggacg gcacccaaag attgaggacc ttcttaggga cagggacctg
      181 gagttcacac ccatcagcat cgacctgaag gagggtgttg cctgcataac aggagccaac
      241 atgggaggaa agaccataag cctcaagctt gtgggactac tgtctgcaat ggcccactac
      301 ggcctctatg ttccggcaga agagatggag ctgggactga gcctatacat caagtcctca
      361 atcggggaca tgcagtccac agacagcgga ctttcaacct tcggaggaga ggtccaggtt
      421 gtaagcgatg caatggaaag ggcagacgaa aagggcctta tcctaataga cgagctggcc
      481 aggggaacaa accccgaaga gggctatgcc atctcaaagg ccatagcaat ctacctcatg
      541 gacaagcctg caatatccct tataacaacc cactacgaca acgtggcgga catagagggg
      601 atccaacacc tccaggttgt cgggctatct gagttggatg ttgacaccct ttcagaggag
      661 atctcagaag gggatggaat ggagataata aacaaatata tggactaccg cctaagggtt
      721 gttgacaaaa ccaccggaat cccaagggat gccataaaca tcgcaaggat aatggggctt
      781 aggacggaga tacttaagat agcggaagag atccttgtaa aagagtaatt cacagatcac
      841 aattcacaat tcacaatttc atgagaggat gtgaacgata tatcggagaa tgtaattgaa
      901 aagaaatcat atgcatttgc aataaatgtt gtgaatgcat ataagaagct tgaaagcaga
      961 agtgaatatg tactttcaaa acaattgctc agatcaggta caaatattgg tgccaatgta
     1021 atggaaggtc tgagagctca aagtagaaaa gattttattt caaagatgaa tatcgctctc
     1081 aaggaagcaa atgaaagtga atactggatc agattactaa tggatacagg atacttaagt
     1141 aagcaagaag acaaatcatt actagatgaa tgcaaggaag tttgtagaat tttatcatca
     1201 atcgttagaa cagcaaacaa taatctcgat aataaagtaa aagaaacaac agaagaatat
     1261 attacaagta ttatagaaga agctaattga ttgttttgca attcttttta tcatttcatt
     1321 gtgaattgag aactgagaac tgtgaattgt catttcattt tgaaatgtga attgagaatt
     1381 gtgttttctc ttttctcttt tcaattgtga attgagaatt gtgaactgtg aattagtaag
     1441 aggggagttg ataccttgtc aaaactaaat ctggacttga aaaaaattga ggcatccaga
     1501 gcctcagcaa gaaacatcgc agaagacata caaaacttca tagacgaaca cacaaccgta
     1561 tccactgaaa gaaccgtagt gaggcttctt ggggtggacg gggtggatga aatcggtaag
     1621 ccacttccaa acgttgttgt ggacaacatt gtcgagggag gcgggcttga aaggggagcg
     1681 gcatactgga ttggaaacgc aatgcttaac cttaagatcc caccacagga gatcgcagaa
     1741 aagattgcaa caggagaggt ggacatcaca aggcttcctg caagggatga ccaggagatc
     1801 agggatgtga tccacacggt tgcagaggaa acagtggaaa agatccttgc caacaagaag
     1861 gtaagggatg actatatcgc agactttgga cctgccaaga cccctaacct gtatgtaatc
     1921 gttgcaacag gaaacatcta cgaggacata gtccaggcaa aggcagctgc aaagcagggt
     1981 gccgatataa tcgcagtaat aaggacaact ggacagtcac ttcttgacta tgttccatat
     2041 ggagctacaa ctgagggctt tggtggaacc tatgcaaccc aggagaactt caaattgatg
     2101 agagaggctc ttgatgaggt tggagaggaa atcggaagat acatcaagct gacaaactac
     2161 tgctcaggcc tttgcatgcc tgagatagcg gcaatgggag cccttgaaag ggttgacatg
     2221 atgcttaatg atgccctcta cggaatactc ttcagggaca tcaacatgca gagaaccctt
     2281 gtagaccagt acttctcaag ggtaataaac ggctttgcag gaatcatcat aaacacaggg
     2341 gaggacaact acctaacaac tgacgatgca gttgaggctg cacacacagt ccttgcatcc
     2401 cagttcatca acgagcagtt tgcccttaag gcaggactcc cagaagagca gatgggactt
     2461 ggacacgcct ttgagatgga gccaaccctt gaggacggct tccttctgga gcttgcacag
     2521 gcccagatgg caagggagat attccccaag gcgccactta agtacatgcc cccaaccaag
     2581 ttcatgacag gaaacatata caagggacat gttcagaatg caatgttcaa catggtatcc
     2641 ataatgacgg gccagggaat ccagcttctt ggaatgctta cagaggctat ccacacccca
     2701 caccttcacg acaggtacct gtccatagag aatgcccagt atgtattcaa caatgcaaga
     2761 cacctaagcg atgagataga gttcaagaag gatggagtta tccagaagag agcccatgcc
     2821 gtacttgagg aagcaagcga ccttctccag cagattgaaa gggaagggct gttccagaca
     2881 atcgagcagg gcaagttcgg tggagtaaga aggatgagaa ccggaggaaa gggacttgct
     2941 ggaatagcaa gaaaggactc aggctacttc aatccattta tccaacttat gcttggaggt
     3001 gacagataat gagtgatatt caaaaagtcg accttacaaa agtaaagcct tacggagata
     3061 caatgaacga tggagcggtc cagctgagct tcagcctccc tgtcccctat ggagacgagg
     3121 caaacgaggc ggcaaggcag cttgcaaaga aaatgggctt tgaggagcct gctgttgtct
     3181 actcccatga ccttggaata gactacacct actttgtggt ttacggcaag acaatccaca
     3241 cagttgacta ctcatcaatc gaggtaccaa aggtggatgt tgatgtcatg gaaaagcatg
     3301 acatcgagga ctacatcaag gacaacatca agagggacgt tgtgatcctt ggggcatgta
     3361 cgggaacaga tgcccacacc gtagggatag acgccatcat gaacatgaag ggttatgcag
     3421 gacactacgg ccttgaaagg tacgagggaa tccaggccta caaccttgga agccaggttc
     3481 caaacgagga gcttgttgcg aaggccatcg agatgaaggc tgacgctctt cttgtgtcac
     3541 aggttgtaac ccagaaggat gtccacatcc caaaccttac ggagcttgtt gagctgcttg
     3601 aggcagaggg aatcagggac aagattgtcc ttgtctgcgg aggccctaga cttagccatg
     3661 agcttgcaaa ggagcttggc tatgatgcag gcttcggatc cggaagctac gcagaggatg
     3721 ttgcaacctt cgttgtaaat gagatagtaa agagaaacct a
//
LOCUS       DASRQF010000225         2863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1530576, whole genome shotgun sequence.
ACCESSION   DASRQF010000225 DASRQF010000000
VERSION     DASRQF010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530576"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="VFD79_05970"
     CDS             <1..934
                     /locus_tag="VFD79_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZH93632.1"
                     /translation="ANSLFEDNAEFGYGMAVATKKIREKLQLAMEEFLTLGLDTEIND
                     YFKEWLEGMNDARATKAVAGKLLPKLDTKFDNERANQLFKFIGELKDHLIKKSVWIFG
                     GDGWAYDIGFGGLDHVLASGEDVNVLVFDTEVYSNTGGQSSKATPTAAVAKFAASGKK
                     IRKKDLGMIAATYGYVYVAQVAMGANMNHTLKAIKEAESYDGPSLVIAYAPCINHGIK
                     AGMGKSIAQEKLAVETGYWHLYRFDPRLTDEGKNPFSLDSKEPTKPVTDFLDSEVRYT
                     SLKLSFPEEAEILYKAAEVAAKARYESYVRMARD"
     gene            1071..1976
                     /gene="murB"
                     /locus_tag="VFD79_05975"
     CDS             1071..1976
                     /gene="murB"
                     /locus_tag="VFD79_05975"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HZH93633.1"
                     /translation="MISYSEYFPKGKFKDIRFDEPLKLHTSFKVGGPADVMIIPSTEE
                     ELIQAVKTCRENDIKYFIMGNESNLLVRDTGIRGVVIKINEGFDNVQVEGTKIMAQAG
                     ALLTAVSKLALRNSLQGMEFASGIPGTIGGAITMNAGAYGGEMKDIVRSVRVMTTDNQ
                     VLQLSNREMNFEYRNSRVKDQDLIVLSVEFELELGELENIEEKMRELTFQRTSKQPLE
                     LPSGGSTFKRPEGYFAGKLIDDSGLRGLRHGDAQVSEKHCGFVVNRGETTYKEVMELV
                     GVIQKTVMDKFGVELEMEIKRVGDD"
     gene            1976..2746
                     /locus_tag="VFD79_05980"
     CDS             1976..2746
                     /locus_tag="VFD79_05980"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZH93634.1"
                     /translation="MYKLTGDFHTHTIYSRGYKKKGTHAKGTIEENVEAAYKLGLDTI
                     VLSEHGPGHYLYGLRVEDLPQIRSEIDRLREIYQPKGLKILLGLESNIVGLDGELDVD
                     DKIIDQLDILLMGYHYGATPVGFTDAVGLYWTSQIGKLIGSREDHAKDIMTQAYLKAM
                     ERYPIDIITHPGSKAKVHIQEVAKGAVKTDTALEISSKHDELSVESLRLIKDIDVKLY
                     IDSDAHEPERVGDIEKGLKKAIDADVDFSRVVNIKWED"
     gene            2751..>2863
                     /locus_tag="VFD79_05985"
     CDS             2751..>2863
                     /locus_tag="VFD79_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019108542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HZH93635.1"
                     /translation="MDFVVITGMSGAGKSQAMKVMEDMGYYCMDNLPPALLS"
BASE COUNT          901 a          547 c          766 g          649 t
ORIGIN      
        1 ggcaaactca ctatttgagg acaatgcaga gttcggatac ggtatggcag ttgcaaccaa
       61 gaagatcaga gagaagctac agttggcaat ggaagagttc ctgactcttg gattggatac
      121 agaaatcaac gactacttca aggagtggtt ggaaggaatg aatgatgcaa gggcgaccaa
      181 ggcggttgca ggcaagcttc ttcctaagct tgacaccaag tttgacaacg aaagagccaa
      241 ccagctgttc aagtttattg gagagttgaa agaccacctg atcaagaaat cagtatggat
      301 cttcggtgga gacggatggg cttatgacat cggattcggt ggacttgacc atgtacttgc
      361 ctcaggagag gacgtaaacg tacttgtatt cgatacagag gtttactcaa acactggtgg
      421 acagtcatcc aaggctactc caactgctgc agttgcaaag tttgctgcat caggtaagaa
      481 gataaggaag aaagacctgg gtatgatagc tgcaacatat ggctatgtat acgtagcaca
      541 ggttgccatg ggagccaaca tgaaccatac gttgaaggcc atcaaggaag ctgaaagcta
      601 tgacggacca tcacttgtaa ttgcatacgc tccatgtatc aaccacggaa ttaaggctgg
      661 aatgggcaaa tcaatagctc aggaaaagct tgccgttgaa acaggatact ggcacctata
      721 cagattcgac ccaagactta ctgatgaagg caagaatcca ttctcattgg attccaagga
      781 gcctactaag ccagtcactg acttccttga ttcagaggtc agatacacat cacttaagct
      841 ttccttccca gaggaagcgg agattctgta caaggcagct gaagttgctg caaaagctag
      901 atacgaatca tacgttagaa tggctagaga ctagtctgga taacaagggt caccatcgag
      961 agatggtgac ctttttatgt ccctccaggt tttttgccca attaggggaa accttatcga
     1021 aatcatgtta taatataagg ataatatata aggaacaggg tgatttagga atgataagct
     1081 acagtgaata ttttcccaaa gggaagttca aggatataag atttgacgag cctttaaagc
     1141 tccatacttc atttaaggta gggggaccgg ctgatgtcat gataattcct tccacagagg
     1201 aagagctgat tcaggcggtt aaaacatgta gagaaaatga tatcaagtat ttcatcatgg
     1261 gtaatgagtc aaatcttctg gtaagggata ctggaattcg aggggttgtc ataaagatca
     1321 atgaaggctt tgacaatgta caggttgagg gaaccaagat aatggcccag gctggagcac
     1381 tactaactgc agtgagcaag cttgcccttc gcaattctct tcagggaatg gagtttgcaa
     1441 gtggaatccc gggaacaatc gggggagcaa taacaatgaa cgcaggagcc tatggagggg
     1501 aaatgaagga tattgtcagg agcgttaggg tgatgacaac tgacaaccag gttcttcagc
     1561 tttccaacag ggaaatgaat tttgaataca ggaacagcag ggtaaaggac caggacctga
     1621 ttgtactttc ggttgaattt gagcttgaac ttggagaact tgaaaacata gaggagaaga
     1681 tgagggagtt gacatttcag cgaacctcaa aacagcccct ggagctaccc agcggtggaa
     1741 gcacattcaa gaggccggag ggatactttg ctggcaagct cattgacgat tcaggtctga
     1801 ggggcttaag acacggagat gcccaggtgt cagagaagca ctgcggcttt gtggtaaaca
     1861 ggggagagac cacctacaag gaggtaatgg agcttgtggg tgtgattcag aagacagtca
     1921 tggacaaatt tggcgttgag ctcgaaatgg agataaaacg agtaggtgat gactgatgta
     1981 caagcttaca ggagattttc atacccacac catttacagc aggggctaca agaaaaaggg
     2041 aacccatgca aagggtacca tagaggagaa tgttgaagct gcctacaagc tgggcctgga
     2101 taccatagtc ctaagcgagc acgggccggg ccactacctc tacggcttga gagtggagga
     2161 cctgccccag atcagaagtg aaatagatag actcagggaa atctaccagc ccaagggctt
     2221 gaagatctta cttggactgg agtcaaacat agtgggcctg gatggggagc tggatgtgga
     2281 tgacaagatc atagaccagc tggacatact ccttatgggc taccactacg gagctacccc
     2341 tgttggattt acagatgctg ttggactcta ctggacaagc cagattggaa agctgattgg
     2401 cagcagggaa gaccatgcaa aagatataat gacccaagcc taccttaagg caatggaaag
     2461 gtacccaatc gatataatca cccaccctgg atcaaaggcc aaggtccata tccaggaggt
     2521 ggcaaaggga gcagtgaaga cagacacggc acttgaaatc agttcaaagc atgatgagct
     2581 ttcagtggaa agtctcaggc tcataaagga tattgatgtc aagctctaca tcgacagcga
     2641 tgcacatgaa cctgaaaggg ttggagatat agaaaaggga ctaaagaagg ccatagatgc
     2701 agatgttgac ttttcaaggg tagttaatat taaatgggag gattgataca atggattttg
     2761 ttgtaataac tggaatgtca ggtgccggaa aatcacaggc aatgaaggta atggaagaca
     2821 tgggttatta ttgcatggat aaccttccgc cagctctctt gtc
//
LOCUS       DASRQF010000226         8749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_258876, whole genome shotgun sequence.
ACCESSION   DASRQF010000226 DASRQF010000000
VERSION     DASRQF010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 8749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8749
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258876"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..989)
                     /locus_tag="VFD79_05990"
     CDS             complement(18..989)
                     /locus_tag="VFD79_05990"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HZH93636.1"
                     /translation="MKTMSMMEAIREAMNKRMREDRNVLLFGEDVGAFGGCFGVSAGM
                     FDEFGPERVRDTPISEGVIIGTAVGAAATGLKPIAELMFMDFATVGADQLVNQAAKMR
                     YMFGGKITLPMVVRLPLGAGISAAAQHSQSLEAWLAHIPGLKVVYPSSPADAYGLMLQ
                     SIDDENPVIFLEHKVLYGASGEVDENMAPIKFGIGDVKREGGDITIVATGKMVHEALA
                     AAEKLSADGIEAEVFDPRTLFPFDKEGLLKSLEKTNKAVIVTEENKRGSFAGEISAII
                     AEDGFDYLDGPVVRVCAMNTPVPFSPVLEQYYVPSVEDIVTAVKKMF"
     gene            complement(990..1970)
                     /gene="pdhA"
                     /locus_tag="VFD79_05995"
     CDS             complement(990..1970)
                     /gene="pdhA"
                     /locus_tag="VFD79_05995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HZH93637.1"
                     /translation="MDLTNEKIIEMYRTMKRIREFETNASRLFAEGSIPGFVHLYLGE
                     EAVATGVCSSMNEDDYITSTHRGHGHIIAKGGESKYMMAELFGKETGYCKGKGGSMHI
                     ADATKGILGANGIVGAGHNIAVGAGLSAKYRESGQVVACFFGDGSTNQGTFHESINLA
                     SIWKLPVVFVCENNLYGISMSQARHQAIQDVADRAVAYNIPGIVVDGNDIFAVYEAAT
                     EAVARARSGQGPTLIECKTYRHKGHFEGDPTTYRPSDEVQEWMAKDPLPRAVAYLMDN
                     EILTEEELKKIDEEIVKEIEDAIKFAEDSPVPSLESIVQDIYTDIVEEVR"
     gene            complement(1986..2342)
                     /locus_tag="VFD79_06000"
     CDS             complement(1986..2342)
                     /locus_tag="VFD79_06000"
                     /inference="COORDINATES: protein motif:HMM:NF021125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lin0512 family protein"
                     /protein_id="HZH93638.1"
                     /translation="MNRYIIEFGMGVDFHGQSVTRAAEKAVLDAVSSSCLCGLEEIMK
                     IKDFNESVGVKVTLAVSRPQEVDVERIKKCLPIGKKTIVAVEGGLVIPGLYIPDFGDK
                     DSSIEAALACVEVELL"
     gene            complement(2545..4473)
                     /locus_tag="VFD79_06005"
     CDS             complement(2545..4473)
                     /locus_tag="VFD79_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HZH93639.1"
                     /translation="MTLDFLDEIKGNWVRYIDHGEISKDVSPMIASSWKRCREAGVDP
                     YGGTGRRIDEDEFEEILQSNRELIEVAKPIMENLNSLVLGTGFVLVLTDKNGVILHVI
                     GEKIVEDEARALNFCPGSSWVEVEVGTNAIGTCIHENKPVQTIGAEHYCIEHHPWTCS
                     AAPIKGDEDDLVGVLDMSGYCLGAHKHTLGIVVAAAYSIQNQLSLKKSYAILDTTVES
                     MSDGMMIVDESFRIKRINKAGDRILGIREGQLDGSDIREYLGEHIFRESDEFTKRIDW
                     DFTVQKTKIPCNVKIAPIEIERGKHGMAILFREMKDVHQTVNVVSGNKATYTFDSILT
                     QNPTMLRAIREAQKFARTRGCVLLEGESGTGKELFAHSIHNHSSRRNGPFVAINCASI
                     PKDLVESELFGYEKGAFTGAVREGKIGKFELANGGTLFLDEIGELPLDLQAKLLRVLD
                     DFTITRVGGKVSKKLDVRLIVATNRDLYEETRRKTFREDLYYRLNVFNVKVPPLRERG
                     DDAAILAKHFVGRLNQTHETDKKISANFLKSIEGLEWKGNVRELENTIHRAFHLCEGN
                     MIDEDLLPEDLCPHEEGEDSYGSLSIKDQERDVIERALIKANGHVIQAGDIIGLSKSA
                     MYRKLKQHRINPKTFKTQ"
     gene            complement(4487..4963)
                     /locus_tag="VFD79_06010"
     CDS             complement(4487..4963)
                     /locus_tag="VFD79_06010"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZH93640.1"
                     /translation="MLKFRNIVTAFLMWNNQVLLLERSQDKEIAPGAWFGVGGHMEPE
                     EINDPYGAAYREILEETGLDKSKIQKLDLKYIIYNRVDDYEIVVNHIFFGDVSAPYVV
                     PNQEGSLHWINVDEALEKLTHPVALKILNHYTNDRRKDMLLGVLDWDEPYVWWYPI"
     gene            complement(5080..5886)
                     /locus_tag="VFD79_06015"
     CDS             complement(5080..5886)
                     /locus_tag="VFD79_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HZH93641.1"
                     /translation="MKLQAALIQMKVVEGKGENLRTAGIMIDQAASEGARLLVLPEMF
                     NVPYDNSYFSEYAEEYPGETTEFLKSKAMELDVYIAGGSIPERDKDRIYNTSYVFSPK
                     GELVGKHRKAHLFDIDVEGGITFKESDVLSPGRESTLFTVDGVKVGLIICYDIRFPEF
                     TRKLALEGAQVVLVPAAFNMVTGPSHWHLSARARALDNQVYMLMCSQSRNMESSYTAY
                     GHSLATGPWGDLVSELDTEEGILHITIDTGTIDKVRNELPLLKHRREELY"
     gene            complement(5899..6795)
                     /locus_tag="VFD79_06020"
     CDS             complement(5899..6795)
                     /locus_tag="VFD79_06020"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HZH93642.1"
                     /translation="MSFIKIKGVIPPMITPFDENDMADYEKHIHNIKKWNQYDLAGYL
                     VLGSNSETVYLNEEEKLELIRLTVENRAEGKLVMVGTGMESTRETITLTNKAAALGAD
                     CALIVTPFYYGGKMNDEALIAYYTDVADKSDIPILIYNVTKYTHVNISPRAVGILSKH
                     PNIIGMKDSSGSIPQLVDYKREVDMKEFNLMVGTVSSWFPALALGVEGTVQALANCCP
                     QECIDIQKLYDEGRMEDSRELYERVFPVNSAVTGAFGIAGLKYVCDRLGFAGGLVRKP
                     LLQLKDDEKVKLDQILETAGII"
     gene            6924..7388
                     /locus_tag="VFD79_06025"
     CDS             6924..7388
                     /locus_tag="VFD79_06025"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HZH93643.1"
                     /translation="MNKYMEQAIIEAEKGIKNRDGGPFGAVVVRDGKIIGRGHNMVVH
                     THDPTAHGEIVAIREASKAMESFDLSDCELFTTCEPCPMCYSAIHWSRIPVVYYGATK
                     EDAAKIGFDDQLLSDILPRQEKLEKIKMVQLDREGCMEVFKKYEEDDERQLY"
     gene            complement(7407..7601)
                     /locus_tag="VFD79_06030"
     CDS             complement(7407..7601)
                     /locus_tag="VFD79_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93644.1"
                     /translation="MISKDDKILDIVSDYPQAEEIFKPYDDIVGKCVMCNHLFETVEE
                     FSDMYGLAMEDLLEKLNRSI"
     gene            complement(7591..>8749)
                     /gene="dpaL"
                     /locus_tag="VFD79_06035"
     CDS             complement(7591..>8749)
                     /gene="dpaL"
                     /locus_tag="VFD79_06035"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015778355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HZH93645.1"
                     /translation="YRDDSKNNMRKDLIQGVRGFHKGFSEYSTTPLHSLESLSEYHGV
                     ESIHVKDESKRFGLNAFKVLGGSYAIGKYLAQKLQMDIRDLDFDYLKSSEVREKIGEL
                     TFVTATDGNHGRGVAWAARELGQKCVVYMPKGSSETRLQNIRNEGAEASIIQGNYNEA
                     VKLSDKMAKEHGWIVVQDTAWKGYEEIPTWIMQGYGTIMDEVVDQLDSTPTHVFLQAG
                     VGSFAGAMLGYILSHYGKDGPRIYIVEPDEANCLYLSSLAGDIRIVDGDMATIMAGLA
                     CGEPNTVSWPMIRDYSDGFISCPDHVAARGMRIMAAPLPGDPQIVSGESGAVGMGLLS
                     HLMERDEYKGVRESMGLGKDSRILLISTEGDTDPVKFRDIVWDGRESEDDK"
BASE COUNT         2183 a         2334 c         1622 g         2610 t
ORIGIN      
        1 acaattgttg cctccatcta gaacattttc ttaacagctg tcacaatatc ttccacgcta
       61 ggtacataat actgctcaag aaccggtgaa aatggaactg gtgtattcat tgcacatacc
      121 cttaccacag gtccatccag atagtcaaag ccatcctcgg cgattatggc tgaaatttct
      181 ccagcaaagc ttcccctctt gttctcctca gttactatta cagccttgtt tgtcttctcc
      241 agggacttca atagaccctc cttgtcaaat gggaaaaggg ttcttggatc aaagacctcg
      301 gcctctattc catcggcgga cagtttttca gcagctgcca gggcctcgtg aaccattttt
      361 cccgtagcaa ctattgtaat gtctcctcct tctcttttta catccccaat gccaaacttt
      421 attggtgcca tgttctcatc aacctctcca cttgctccgt agagaacctt gtgctcaagg
      481 aatattactg gattctcgtc gtctattgac tgaagcatca gaccataggc atcagcagga
      541 cttgatggat atacaacttt aagtcctggt atatgggcca gccatgcctc cagtgactgg
      601 gagtgctggg ctgccgcact tattcctgct ccaagtggta gtctcacaac catcggcaaa
      661 gtaatttttc caccgaacat atatctcatt tttgccgcct gattcaccag ctggtcagca
      721 cccacagttg caaagtccat aaacattagc tctgcaatgg gttttagtcc tgtagctgcg
      781 gcacctactg cagtaccgat tataacaccc tcagaaattg gggtatccct caccctctct
      841 ggaccaaact catcaaacat acctgcggag accccaaagc aacctccaaa cgctccaaca
      901 tcctcaccaa aaagcaggac attcctatct tctctcattc gcttgttcat agcctctctg
      961 atggcttcca tcatactcat ggttttcatc tatcttacct cctctacgat atccgtataa
     1021 atatcctgaa caatcgactc taaagaaggc accgggctat cctctgcaaa tttaatggca
     1081 tcctctattt ccttgactat ttcctcatca atcttcttca gttcttcctc tgtgagtatt
     1141 tcattatcca ttaagtaagc aactgctctt ggaagaggat cctttgccat ccactcctga
     1201 acctcatctg atggtctata ggttgttggg tcgccctcaa agtgaccttt atgtctgtag
     1261 gtcttgcatt ctatcagggt tggaccctga ccacttcttg cccttgcaac cgcttctgtt
     1321 gcagcctcat agacagcgaa tatgtcattt ccatcaacta cgataccagg aatattgtat
     1381 gccactgccc tgtctgcaac atcctgtatt gcctggtgtc ttgcctggga cattgatatt
     1441 ccgtaaaggt tgttttcaca cacgaataca acgggaagct tccaaatgct tgccaggttt
     1501 attgactcgt ggaaagtccc ctggtttgtg gatccgtcac caaagaaaca ggctacaacc
     1561 tgccctgact ccctgtattt tgcacttaag cctgctccaa ctgcaatatt gtgacctgcc
     1621 ccaacaattc cgttggcccc caggataccc ttggttgcat cggcaatatg cattgatccg
     1681 cccttgccct tgcagtatcc ggtttccttg ccgaaaagct ctgccatcat gtactttgat
     1741 tctccgcctt ttgcaattat gtgaccatgg cctctgtgag tacttgttat ataatcatcc
     1801 tcattcatgc ttgagcagac tcctgttgcc acagcctcct cacctagata taggtgaacg
     1861 aagccaggta tactgccttc tgcaaaaagc ctgcttgcat ttgtctcaaa ctccctgata
     1921 cgtttcattg tcctgtacat ttcaatgatt ttttcattag tcaaatccaa gaaaatccct
     1981 ccttgttaaa gcaactctac ctcaacacac gccaatgcag cctctatact gctgtccttg
     2041 tccccaaagt ctggaatgta caagcctgga atcacaagac caccttcaac tgccacaatt
     2101 gtctttttcc caatgggaag acatttcttt attctctcca cgtctacctc ctggggcctt
     2161 gatacagcta gggtgacctt gacaccaaca ctctcgttga aatccttgat tttcataatc
     2221 tcttcaagtc cacataaaca gctactggaa actgcatcca atacagcttt ctccgcagct
     2281 cttgttacgc tctggccgtg aaaatcgact cccatcccaa actcgatgat ataccgattc
     2341 atgatcatca cctccaattg aatcccttgt actggtccct cctttcaatt gggaatcatt
     2401 acatcgtcaa tatataattg caattaccat gccaaaatgc tttctccttt tactgcgcta
     2461 ttgcgtgcct ctggggacct gggaaattcc ctccaaatag aaaattccca caatcgggaa
     2521 ttttgtttcc cgattgtggg aattttattg agtcttgaag gtctttggat ttattctatg
     2581 ttgcttaagc tttctgtaca tggccgattt gcttaagcca ataatgtcac cagcttggat
     2641 tacgtgacca tttgctttta ttagggccct ttcaataaca tccctctcct ggtccttaat
     2701 ggaaaggctc ccgtatgaat cctccccctc ctcatgagga cagagatctt caggaagcag
     2761 gtcttcatct atcatattgc cctcacagag atgaaatgct ctgtgaatag tgttttcaag
     2821 ttcccttaca ttacccttcc actcaagccc ttcaatgctc tttagaaagt tcgcagatat
     2881 ttttttatca gtttcatgtg tctggttgag cctccccaca aaatgctttg ccagaatggc
     2941 ggcatcatcc cccctctccc ttagaggagg aacctttaca ttaaatacat tcagtcggta
     3001 atacaggtct tcccggaagg tcttccttct ggtctcttca tagaggtccc tgttggttgc
     3061 aacaatcagt ctcacatcca gcttcttgga aacctttcct ccaaccctgg ttattgtaaa
     3121 gtcatccaaa acccgcaaaa gcttggcttg caggtcaaga ggaagctcac caatctcatc
     3181 aaggaagagg gttccaccat tggccagctc aaattttcct attttccctt cccttacagc
     3241 cccagtaaat gcgccttttt catagccgaa aagctcgctt tccacaaggt ccttgggtat
     3301 tgatgcgcag ttaatggcta caaagggtcc attccttcta ctgctatgat tatgtatgga
     3361 atgtgcgaaa agctcttttc cagtcccact ttcaccctct aaaagcacac agcctcttgt
     3421 tcttgcaaac ttctgcgcct cccttatggc ccttagcatg gtgggatttt gtgtaagtat
     3481 actatcaaaa gtatacgttg ccttgttgcc cgagaccaca ttcactgtct ggtggacatc
     3541 cttcatttcc ctgaaaagga tggccatacc atgctttccc ctctctattt ctatgggtgc
     3601 tatcttcacg ttacagggaa tcttagtctt ttgaacagta aagtcccaat caatcctttt
     3661 agtaaattca tcgctttccc tgaatatatg ctctcccagg tattccctta tgtcacttcc
     3721 atccaattgg ccttctctta tccctaaaat cctgtctcct gccttattta tcctctttat
     3781 cctaaagctt tcgtccacta tcatcatacc atctgacatg gattcgacgg tggtatccag
     3841 tattgcatag gattttttca gtgacagctg attctggatg gagtatgcag ccgcaaccac
     3901 tatgccaagg gtgtgcttgt gtgcacccag gcagtagcct gacatatcca acacccccac
     3961 caaatcatcc tcgtcgcctt ttattggtgc agctgaacag gtccatggat ggtgctctat
     4021 gcagtaatgc tccgctccta ttgtttgtac aggcttattc tcatggatac aggttccgat
     4081 tgcatttgta cccacttcta cttcaaccca gcttgaaccg ggacagaagt taagagccct
     4141 ggcttcatcc tcaactatct tctcaccgat cacatgaagt attacaccat tcttatctgt
     4201 aagcacaaga acaaagcctg tccctagaac aagactgttt aagttttcca ttattggctt
     4261 tgcaacttca attagctccc tgtttgattg caggatttcc tcaaactcat cctcatcgat
     4321 ccttctcccg gttccaccat aggggtcaac tccagcctcc ctgcatcgtt tccaagagct
     4381 ggcaatcatt ggcgaaacat cctttgagat ttctccatga tctatatatc tcacccaatt
     4441 acctttaatt tcatcaagaa agtccaatgt cattctatca acctccctat atggggtacc
     4501 accaaacata tggctcatcc cagtctagaa cgcccaacag catatccttt ctacgatcat
     4561 tggtatagtg gttcaatatc ttcaaggcaa caggatgtgt gagcttctca agggcttcgt
     4621 caacattgat ccaatgtagc gacccctctt gatttggaac cacatagggt gccgacacgt
     4681 ctccaaagaa tatgtgattt acaactatct cataatcatc taccctattg taaataatgt
     4741 acttcaagtc aagcttctga atctttgact tatcaagacc tgtttcctcc aatatctccc
     4801 tgtaggctgc tccgtagggg tcatttattt cctcaggctc catatgccct ccaactccaa
     4861 accatgctcc tggagcaatt tccttatcct gacttctttc aagcaataat acctggttat
     4921 tccacatcag gaacgctgtg actatatttc tgaacttaag catatcccac cctccttccc
     4981 taaacatata cccttttttc aacacaacaa tacataatac cgatatagtg ggaaagtgtg
     5041 gaaaagccct gccaaagcag ggctgatctt gtcctagtct taatatagtt cctctcttct
     5101 gtgctttaga aggggaagct catttctcac cttgtcgatt gtaccagtat caattgttat
     5161 gtggagtata ccctcttcag tatccagttc tgaaaccagg tctccccacg gtccagttgc
     5221 cagggaatgg ccataggccg tataggagct ttccatattt cttgactgtg agcacatcag
     5281 catgtagacc tggttgtcaa gagcccttgc cctcgctgaa agatgccaat gagaaggtcc
     5341 tgtgaccatg ttgaatgcag ccggtacaag taccacctgg gcaccctcaa gagccagctt
     5401 tctggtaaat tcagggaatc ttatatcata gcagataatt agtcccacct tgaccccgtc
     5461 cacagtgaac agagtggatt cccgacctgg agaaaggaca tccgattcct taaaggttat
     5521 tcctccctct acgtcaatat caaaaagatg agcctttctg tgctttccaa caagttctcc
     5581 cttgggtgag aatacatagg aggtgttgta gatccggtct ttatcccttt caggtatgga
     5641 gcctccagca atgtatacat ccagttccat tgccttgctt tttagaaatt ccgtagtctc
     5701 tccgggatat tcttcagcgt actcggaaaa ataactattg tcatatggaa cattgaacat
     5761 ttcaggaagc acaagcaatc ttgcaccttc agaggctgcc tggtcaatca ttatacctgc
     5821 agtccttaga ttctctccct ttccttccac aaccttcatc tgtattagtg cagcttgaag
     5881 cttcatcgtc tcaccccttt agattattcc agccgtctca agtatctgat caagctttac
     5941 cttctcatca tccttaagct gtagaagtgg ctttcttaca agacctccgg caaaacccag
     6001 cctgtcgcag acatatttca ggcctgcaat cccaaatgca cctgtcacag cggaattaac
     6061 agggaagacc ctttcataaa gttctctgga atcctccatc cttccttcat catataattt
     6121 ctggatatct atgcattcct gtgggcagca gtttgcaagt gcctggacag tcccttcaac
     6181 tcccagtgca agagccggga accatgatga tactgttcca accataaggt tgaactcctt
     6241 catgtcaacc tccctcttgt agtcaacaag ctgcgggatg ctccctgagc tgtccttcat
     6301 cccgatgatg ttggggtgtt tggaaaggat tccgactgcc cttggtgaaa tatttacatg
     6361 ggtatacttt gtaacattat atatgagtat tggaatatct gatttgtcag caacatcagt
     6421 atagtaggcg atcagggctt cgtcattcat ctttccgccg taatagaagg gcgttactat
     6481 aagtgcgcag tctgctccca gggctgcagc cttgtttgta agggtgattg tctccctggt
     6541 tgactccatg cctgttccca ccatgacaag cttgccctct gccctgttct ctacagtcag
     6601 ccttatgagc tcaagctttt cctcctcgtt aagataaact gtttcactgt tggatccaag
     6661 taccaaatag ccagcaaggt catactggtt ccatttctta atattgtgaa tatgtttttc
     6721 atagtccgcc atgtcatttt catcaaaggg tgtaatcatc ggaggtatta ctccctttat
     6781 ctttataaaa ctcatcaaaa tctccccttt acctagtaat gtgtagatta tatcacttta
     6841 tagcaataat ttacagtggt cttccttgta ttttagtcca aatactgggt atatatgtag
     6901 tggccaggaa aggggaagat aagatgaaca aatacatgga acaagctata atagaagctg
     6961 aaaagggaat taaaaatagg gatggcggcc cctttggagc cgtcgttgta agggacggga
     7021 agatcattgg tagaggccac aatatggtgg tccataccca cgacccgact gcccacggtg
     7081 agattgtggc cataagagag gccagcaagg caatggaaag ctttgacttg tccgattgtg
     7141 agctcttcac cacctgcgaa ccctgtccca tgtgctactc tgcaattcac tggtccagaa
     7201 ttcctgtcgt gtattatggg gcaacaaagg aggatgcggc gaaaatagga ttcgacgacc
     7261 agctcctgtc tgatatactg ccaagacagg aaaagcttga aaaaataaaa atggtccagc
     7321 tggacaggga aggatgcatg gaagtattca aaaaatatga agaggatgat gaaaggcagc
     7381 tgtattgaaa tcagctgcct tctacattaa atacttctgt taagcttttc aaggaggtct
     7441 tccatagcaa gtccatacat gtcagaaaac tcttcaactg tctcgaatag gtgattgcac
     7501 attacgcatt tcccgactat atcatcgtag ggcttgaata tttcctcagc ctggggatag
     7561 tcggatacta tatccagtat cttgtcatcc ttacttatca tcctcgctct cccttccatc
     7621 ccaaactata tctcggaatt taacagggtc ggtatcccct tcagtactga tcagcaaaat
     7681 tctagagtcc tttcccagac ccatggattc ccttactccc ttgtactcat ccctctccat
     7741 aagatgcgaa agcaggccca ttccaacagc tccagattca ccagaaacaa tttgagggtc
     7801 tcctggcaag ggggctgcca taatcctcat gcccctggct gccacatgat ctggacagga
     7861 tataaaccca tcgctgtagt cccttatcat tggccagctg actgtattgg gttctccaca
     7921 ggcaagaccg gccattatgg ttgccatgtc tccatccact atcctgatat cacctgccag
     7981 ggaagatagg tataggcaat tggcttcatc aggctccaca atatatatcc ttggcccatc
     8041 cttaccgtaa tgtgacagga tataaccaag cattgcccct gcgaatgaac ctactcccgc
     8101 ctgcaaaaag acgtgggttg gagtactatc aagctggtcc accacttcgt ccattatggt
     8161 tccgtagccc tgcattatcc aggttggaat ttcctcatat cccttccagg cagtgtcctg
     8221 gaccacaatc cagccgtgtt ccttggccat cttatctgaa agcttgacag cctcattgta
     8281 atttccctgg attattgatg cctccgctcc ctcattcctg atattctgaa gccttgtttc
     8341 tgatgatccc ttaggcatgt atacaacgca tttctgtccc agctcccttg ctgcccaggc
     8401 aactcctctc ccatggtttc catctgttgc agtgacaaag gtaagctcac cgattttttc
     8461 tctgacctca cttgacttta ggtaatcaaa gtccaggtcc ctaatatcca tttggagctt
     8521 ttgtgccaga tatttgccga tggcatatga acccccaaga accttgaagg catttagacc
     8581 aaaccgttta ctctcatcct taacatggat ggactccaca ccatgatact cagacaggct
     8641 ttcaagacta tggagtggtg ttgtggaata ctctgaaaaa cccttgtgaa aacctcgaac
     8701 cccctggatt agatccttcc tcatattatt tttgctgtca tccctatac
//
LOCUS       DASRQF010000227         2334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_112909, whole genome shotgun sequence.
ACCESSION   DASRQF010000227 DASRQF010000000
VERSION     DASRQF010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112909"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..204)
                     /locus_tag="VFD79_06040"
     CDS             complement(67..204)
                     /locus_tag="VFD79_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HZH93646.1"
                     /translation="MEDIYSSVANLGFPIVISVYLLVRIEGKLDELTKSITELAHAIA
                     K"
     gene            complement(255..473)
                     /locus_tag="VFD79_06045"
     CDS             complement(255..473)
                     /locus_tag="VFD79_06045"
                     /inference="COORDINATES: protein motif:HMM:NF022597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HZH93647.1"
                     /translation="MVKTKLEMDFLDDQNKNHRISMADPRADLSDVEVFEAMDTLLAA
                     GIFQSRNGSLVDKVGARIVTTTVDEMTV"
     gene            complement(505..729)
                     /locus_tag="VFD79_06050"
     CDS             complement(505..729)
                     /locus_tag="VFD79_06050"
                     /inference="COORDINATES: protein motif:HMM:NF019487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HZH93648.1"
                     /translation="MAIAVMAEKKTLKLELDAGVVDGRQRISSKSYSNVKLDATDDNI
                     HASGEVLAGLQTLPLMKVKKVEESIITNEG"
     gene            complement(851..>2334)
                     /locus_tag="VFD79_06055"
     CDS             complement(851..>2334)
                     /locus_tag="VFD79_06055"
                     /inference="COORDINATES: protein motif:HMM:NF014871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HZH93649.1"
                     /translation="IPANLTEGESVVEVMNAMEFDAMVPGNHDFNFGYERLLELKAMS
                     EFDILGANIVKEDETSDFDPYLVYEMENGMRVGVFGIATEETKTKSHPNNTIGIEFAD
                     VIETGNAAVEALAEMDVDLVVALIHLGIDGSSADTAYTLADNVEGIDLIVDGHSHHVT
                     GEERNGAYIVQAGYYLIHIGVVNVSMVDGEPVFESTQHPYEELKDLEADEYIAGLIAE
                     TTAPIEEMQEEVIGFTTVALDGVRENVRAGETNLGNLITDAMIAASGADVALTNGGGI
                     RASIDEGDITLGEVLEAFPFTNVLTNIEVTGADLMAALEHGVKDYPAAAGHFPHVSGM
                     SYTFDPNQEVGSRITELMIGDEPVDLEATYMLVTNDFLAAGGDGYEMFGGEILAYSGL
                     LSEVLETYLRANPEISPQVEGRIVALPLVEVPEPEPAPEPEPTPVPEPTPAPADIAYT
                     VKSGDWLSKIGMAYGVDWRVLADHNNLANPNLIFPGQIIMIPQ"
BASE COUNT          572 a          610 c          453 g          699 t
ORIGIN      
        1 gacacacttt aactccatat aatgtactga aaggcagaat cacttggatt ctgcctttct
       61 cattgcctat tttgctatgg cgtgggccag ctccgtgatg ctctttgtaa gctcgtccag
      121 cttcccctct atcctcacaa gaagatagac agagattact attggaaagc ccagattggc
      181 aacactggaa tagatgtcct ccatatcatc ccccctaaca aaatgttacc gcggggtacg
      241 tccccgcggt aacattacac tgtcatttca tccactgttg tggtcactat cctagctccc
      301 accttgtcaa caaggcttcc gttcctggac tggaagatac ctgctgcaag gagtgtgtcc
      361 atagcctcaa atacctccac atctgaaagg tcagcccttg gatctgccat gctgatccta
      421 tggttcttgt tctggtcatc cagaaaatcc atttcaagct ttgtctttac catctaatca
      481 cctccactct agatttcaca cgaattaacc ctcgtttgta ataattgact cctccacctt
      541 cttgaccttc atcagtggaa gggtctgaag ccctgcaagg acctcccctg atgcgtggat
      601 gttgtcatca gtagcatcca gcttcacatt gctgtaggat tttgaagaaa tcctttgtct
      661 gccatcaaca acccctgcat caagctcaag cttcagtgtc ttcttttctg ccataactgc
      721 tatcgccatt gtctcacccc ccttctacct attacattgc agatgggagg aaaatgtacc
      781 tatgtaatat taaaaaaagt caaaaaaaat tagaggggct ttcgcccctc aggtattcta
      841 ctggtatctt ctattgtgga atcattatga tctgacctgg gaagatcaga tttggattcg
      901 caagattgtt gtggtctgca agaactctcc agtccactcc ataggccata ccaatctttg
      961 ataaccagtc tcctgacttg acagtatatg ctatgtcagc aggtgctggt gttggctcag
     1021 gaaccggtgt aggctctggc tctggtgctg gctcaggttc aggaacctca accaatggaa
     1081 gggctactat ccttccctca acctgtggac tgatttcagg gttggctctt agataggttt
     1141 caagtacctc tgaaagaagt cctgagtatg caaggatctc tccgccaaac atttcatatc
     1201 cgtctccacc tgctgcaagg aagtcatttg ttaccagcat atatgtcgct tccagatcaa
     1261 caggctcatc gcctatcata agctctgtaa ttcttgatcc aacttcctgg ttgggatcaa
     1321 aggtgtagct cattccactt acatgtggga agtgtcccgc agctgcagga tagtccttta
     1381 ctccatgctc aagggctgcc ataaggtctg ctccagttac ctcaatgttt gtcaatacat
     1441 tggtgaatgg gaatgcttcc agtacctcac caagagtgat atctccctca tcgatgctgg
     1501 ctcttattcc accaccgttt gtaagggcaa catccgctcc tgatgcagct atcatcgcat
     1561 ctgtaatcaa gttgccaagg ttggtttctc cagctcttac attctctctt acgccgtcaa
     1621 gagccacagt ggtaaatcca attacctctt cttgcatttc ctcaattggt gctgttgttt
     1681 ctgcaattag tccagcgata tactcatcgg cctcaagatc cttaagctct tcataaggat
     1741 gctgagttga ttcaaataca ggttctccat caaccatgga tacatttacg actccaatat
     1801 ggataaggta gtatccagcc tgtacgatat atgcaccgtt tctttcctcg cctgttacgt
     1861 ggtgactatg gccgtctact atcaggtcga ttccttcaac gttgtcagca agggtgtaag
     1921 ctgtgtcagc tgatgatccg tcaattccaa ggtggattag tgcaaccaca aggtcaacat
     1981 ccatctcagc tagggcctca actgcagcat ttcccgtctc gatcacatct gcaaactcta
     2041 ttcctattgt attgttagga tgtgactttg tttttgtctc ttcagtggct atgccaaata
     2101 ctccaactct catgccattc tccatctcat aaaccaaata tgggtcaaaa tcggatgttt
     2161 catcctcttt gactatattg gctccgagaa tatcaaactc tgacattgcc ttcagctcaa
     2221 gcaatctctc ataaccgaag ttgaaatcat gattgccggg aaccattgca tcaaactcca
     2281 ttgcattcat tacttcaacc actgattcac cttcagtaag gttggcaggg attg
//
LOCUS       DASRQF010000228         4980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1522628, whole genome shotgun sequence.
ACCESSION   DASRQF010000228 DASRQF010000000
VERSION     DASRQF010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522628"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VFD79_06060"
     CDS             complement(<1..789)
                     /locus_tag="VFD79_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HZH93650.1"
                     /translation="MWNNKSIGEAMEIKLDNYLPEYPEFSKFIRRAPKREFTLTQKET
                     EVALKNALRYVPEDLHEELAPEFLEELLTMGRIYGYRFRPNEIIYGKPIDEYKGKCIE
                     GKAFQVMIDNNLSFDVALYPYELVTYGETGQVFQNWMQYRLTMKYLEELTDDQTLVLQ
                     SGHPLGLFKSHKNSPRVIITNALMIGAFDDQEHWIKAQAMGVANYGQMTAGGWMYIGP
                     QGIVHGTYNTILNAGRLKLGIPEDKDLAGHMFVSSGLGGMSGAQG"
     gene            complement(803..2059)
                     /gene="hutI"
                     /locus_tag="VFD79_06065"
     CDS             complement(803..2059)
                     /gene="hutI"
                     /locus_tag="VFD79_06065"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HZH93651.1"
                     /translation="MKASLVIKNIKTLVTVRGGEKPRTGEAQGKAGIIENGIVAVKDG
                     KIIFVGEGGLPEGIEIDDNTVVIDGSGKTVTPGLIDCHTHLVHGGSRENELAMKLEGR
                     GYLEILAAGGGIHSTTRATEKATPEELYEKAWKSLDTMLGFGVTTVEAKSGYGIGSFE
                     TELKQIDVANRLNQDHPVDILPTFMGAHAVPADYKENPDQFVDKLVDEMIPHIADNKL
                     TKFCDVFCEEGVFTVDQSRRIILAAKENGLIPKIHADEIVSLGGAELAAETGCISAEH
                     LIGASDQGIEDMAKAGTIAVLLPGTSFNLKKDGARARDMIAAGVPVAVSTDYNPGSCP
                     SENLQLMMTLASHDLKMLPEEVITAVTLNAAAALGISDHTGSLEIGKNADIAIFDAPN
                     LDYMIYHFGINHTDKVIKGGKVVFEK"
     gene            complement(2285..3982)
                     /locus_tag="VFD79_06070"
     CDS             complement(2285..3982)
                     /locus_tag="VFD79_06070"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZH93652.1"
                     /translation="MDFYVLGSIFEKLAPLSAITDREGKILWTSSSLEGLFSLEEKDM
                     EEVLGLERGGLWNLPDGKEIEIFLKDRDYRVRTFKAGEYTFIEFQEIGDYKDPRVRAD
                     SLLQVIENLYDGVLLSDKEGRVKVYNKSMEDLEKRRAEDMLGKYIWDAYGYSDKDKSE
                     HMEVFKTGKPIINKYAAHAYNEGKPVYKSYSTIPIKEGDKTIGVFSISKDETKLQNLL
                     SQIMDLKRESYGLEEREGAFLNGTRFTFSDIIGSSPAIKKVIREAQAIAWLDNSILLV
                     GDTGTGKEVFAQSIHNFSKRKGHPFIGINCSAIPENLLESILFGAVKGAYTGAVDSPG
                     LFEEAGEGTLFLDEIDSMPINMQTKLLRVLQERNTRRVGGKDSYPIRCRVISAMNTDP
                     YKLIDAGKLRLDLFYRISGYNLYIPPLRDRDEDIFHISHHYIKKYNLVMDKVISSIGE
                     DLKNMMKTYPWPGNIRELEHFIENTMVRSGEEEKVLRLENIPDYILEAMKWSDSGIQE
                     REVSTENLQDSLDGLEKRLIKQALGKNFWNVTRTAKALGITRQSLIYRMRKYDIDRD"
     gene            complement(4001..4891)
                     /locus_tag="VFD79_06075"
     CDS             complement(4001..4891)
                     /locus_tag="VFD79_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005883995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZH93653.1"
                     /translation="MKFVTYLNRNKEHPGVLSRDERSVYSLDRIFGVGRFPSMVDFIE
                     KADEDHIRKVEKTLKEESIKALSLEEVSILSPITRPVHDIICVGVNYADHLKESQSAI
                     KDGNLDKVTKPVYFSKRTTRIMGPDEDIKGEFDLDGEFDYEVELAVVIGKMGRDIPED
                     RVEDYIFGYSIFNDLSSRRLQREHIQWYKGKSLDGYSVMGPVILHKSQLPFPMEVDVI
                     SRVNGEVRQSSNTKMLINDIATLISDFSKGITLEPGDIIATGTPAGVGMGLKPPRFMK
                     RGDEIQCEIPQIGVLTNKIV"
BASE COUNT         1133 a         1306 c          963 g         1578 t
ORIGIN      
        1 accctgtgca ccgctcattc caccaagtcc ggagcttaca aacatgtggc ctgcaaggtc
       61 cttgtcctca ggaattccaa gcttaagtct tcctgcatta aggattgtat tgtaggtacc
      121 atgaacgatc ccctgtggtc cgatgtacat ccatccacct gctgtcatct ggccatagtt
      181 tgcaacaccc attgcctgag ccttgatcca gtgttcctgg tcgtcaaatg ctccaatcat
      241 caatgcattt gtgattatga cccttggact gtttttgtgt gatttaaaca gacccagtgg
      301 atgacctgac tgaagtacaa gtgtctggtc gtcagtgagc tcctcaagat atttcattgt
      361 cagcctgtac tgcatccagt tctggaatac ctgacctgtc tcaccatagg ttaccagctc
      421 atatggataa agggccacat caaaggatag gttgttgtct atcataacct ggaatgcctt
      481 gccctcgatg cacttaccct tgtactcatc aattggcttt ccataaatta tctcatttgg
      541 tctgaagcga tagccgtaga tcctgcccat tgtaagaagc tcctccagaa actccggagc
      601 caactcctcg tgcaggtctt caggaacata tctaagggca ttcttaaggg ccacttctgt
      661 ttccttctgg gtcaatgtga actccctttt tggtgccctt cttatgaact tgctgaattc
      721 ggggtattct ggaagataat tatccagctt gatttccatt gcctcaccaa tacttttgtt
      781 attccacata ttacatcctc ctttattttt caaatactac ttttccgccc ttgatgacct
      841 tgtcagtgtg atttatacca aagtggtaaa tcatgtagtc caggtttgga gcgtcaaata
      901 ttgcaatgtc agcattctta ccaatttcaa gtgatccggt gtggtctgag atcccaaggg
      961 ctgcagctgc attaagtgtg accgctgtta ttacctcttc aggcagcatc ttaaggtcat
     1021 gggatgccag tgtcatcatc agctgaaggt tttcgctggg gcagcttcct ggattgtaat
     1081 cagtggaaac cgcaacaggt actccagcag ctatcatatc cctggccctt gcaccatcct
     1141 ttttaaggtt aaagcttgtt ccaggtagca ggacagctat tgtaccagcc tttgccatat
     1201 cctctattcc ctggtcgctg gccccaataa ggtgctcagc ggatatgcag cctgtttccg
     1261 ctgccagttc cgctccacca agtgatacta tctcgtctgc atgtatcttt ggaatcagtc
     1321 cattctcctt tgccgcaagg attatccttc ttgactggtc aacagtgaat actccctctt
     1381 cgcagaatac atcacagaac ttggtcagct tgttgtctgc tatgtggggg atcatttcat
     1441 ctaccagctt gtcaacaaac tggtctggat tttccttgta gtctgctggt actgcatgtg
     1501 cccccataaa ggttgggagg atgtccacag gatggtcctg gttcagtctg tttgcaacat
     1561 caatttgctt taattcagtc tcgaagcttc caattccata gcctgacttt gcttccactg
     1621 tggttacccc aaagcccagc atggtgtcaa ggctcttcca tgccttttca tacagctcct
     1681 ctggtgtcgc cttttctgtg gcccttgtag ttgagtggat ccctcctcca gctgcaagga
     1741 tttctaggta tcccctgcct tccagcttca tggcaagctc attttccctg cttcctccat
     1801 gaaccaggtg ggtgtggcag tcaatcaatc caggggtaac ggtctttcca gatccgtcta
     1861 ttaccaccgt attatcatcg atttcaattc cttcaggcaa gcctccctca cctacaaaaa
     1921 ttattttacc gtccttcact gcaacaatgc cattctcgat tattccagcc tttccttggg
     1981 cctctccggt tcttggcttt tctcctcccc ttactgttac cagggtcttt atgtttttaa
     2041 tgaccaatga tgccttcata taattcctcc tctcatttcg ctcaataaat atccacctta
     2101 attctattgc aatttccgtg ccaggtttta tcatctctac aaacacctaa aacagtagat
     2161 tcttgaatat ctcaaattag aaaacggcag atagtgtttt attttcaaac accatctacc
     2221 gtttttcttg atttttattg ttttattttc aatcactttg ttttatttta aaacatatct
     2281 ggatttaatc cctgtctatg tcgtattttc tcattctgta gatcaggctc tgtctagtga
     2341 ttccaagggc tttggcagtc ctggtcacat tccagaaatt ttttcccagg gcctgcttta
     2401 tgagcctctt ttccagtccg tccagactgt cctgaagatt ctcagttgag acctcccttt
     2461 cctgaatgcc agagtcgctc cacttcattg cctcaagaat ataatccggt atattttcaa
     2521 gccttaggac cttctcttcc tctccacttc tgaccatggt gttttcgata aagtgttcca
     2581 gttcccttat gttgccaggc caggggtagg tcttcatcat gttcttcagg tcctctccaa
     2641 tacttgaaat gaccttatcc atcaccaggt tgtacttctt gatgtagtga tggctgatat
     2701 gaaaaatatc ctcatccctg tctcttaggg gtgggatgta caagttatag ccagatattc
     2761 tgtagaagag gtcaagtctt agcttgcctg catcaataag cttgtagggg tctgtgttca
     2821 ttgcacttat gaccctgcac ctgattgggt agctgtcctt gcccccaacc ctccttgtat
     2881 ttctctcctg gagtacccta aggagctttg tctgcatgtt tattggcatt gaatcaatct
     2941 catcaagaaa aagtgtccct tcacctgcct cctcaaaaag tcccggtgaa tccacagctc
     3001 ccgtgtaggc ccccttgaca gcaccaaaaa gtatgctttc cagaaggttt tcaggaattg
     3061 cagagcagtt tatcccaatg aatgggtgtc cctttctctt gctgaagttg tggatgctct
     3121 gggcaaatac ctcttttccc gtccctgtat caccaaccag aagtatgctg ttgtcaagcc
     3181 aggcgattgc ctgtgcttcc cggatgacct tctttatggc tggacttgac ccgattatgt
     3241 ccgaaaaggt gaaccgggta ccgttgagaa aggctccctc cctttcctca agtccgtagg
     3301 attccctttt aaggtccatt atctgggaaa ggagattctg aagcttggtc tcatccttgc
     3361 tgattgaaaa gactccaatg gtcttgtccc cttccttgat tgggattgtt gaataggact
     3421 tgtagaccgg ctttccttca ttgtatgcat gggcggcgta cttgttgatt atgggcttgc
     3481 cagtcttgaa tacctccata tgctcggact tgtccttgtc gctgtagccg taggcatccc
     3541 agatatactt gccaagcatg tcctctgccc ttcttttctc caggtcctcc atggacttgt
     3601 tgtagacctt tacccgccct tccttatcgc tgagcaggac tccatcatac aggttctcta
     3661 tcacctgtaa aagactatct gcccttaccc tggggtcctt gtagtctcca atctcttgga
     3721 actcgataaa agtgtattct cctgccttga aggtccgaac ccggtagtcc ctatccttta
     3781 gaaagatctc tatttccttt ccatcgggaa gattccaaag acctcccctt tcaagaccca
     3841 ggacctcctc catatccttt tcttcaaggg aaaaaagccc ttcaagggaa gagctggtcc
     3901 acaatatttt tccttcccta tcggtgatgg ccgaaagggg tgccaacttt tcaaatatac
     3961 ttcctaaaac ataaaagtcc atcaaaaacc tccgataagc ttacactatt ttattggtca
     4021 aaactccaat ctggggaatc tcacactgga tttcatcgcc cctcttcatg aatcttgggg
     4081 gtttaaggcc cattccaact cccgccggtg ttcctgtggc gattatatct ccaggctcca
     4141 gggtaatccc ctttgagaaa tccgaaatca gtgttgctat gtcatttatc agcatttttg
     4201 tattggagct ttgtctaacc tctccattga ccctggaaat cacatcaacc tccattggga
     4261 atggaagctg ggatttgtgg agtatgaccg gccccataac tgaatagcca tcaaggcttt
     4321 tccccttgta ccactgaata tgttcccttt gaagccttct tgatgacagg tcattgaata
     4381 tagaatagcc gaagatgtaa tcctcaaccc tatcctccgg tatgtccctg cccatctttc
     4441 caatgacaac agccaactca acctcgtagt caaactcccc gtcaaggtca aattctccct
     4501 tgatatcctc atccggtccc attatccttg tggtcctttt tgaaaaatac acaggcttgg
     4561 tcaccttgtc aagatttcca tcctttattg cagattggga ttccttcagg tggtcggcat
     4621 agttgacgcc aacacagatg atgtcatgta ccggcctggt gattggtgac aaaattgaga
     4681 cttcctcaag ggaaagagct ttgatggact cctccttcag ggttttctca accttcctta
     4741 tatggtcctc gtcagccttc tcaataaagt cgaccatgga aggaaatcta cctactccaa
     4801 aaatcctatc aaggctgtat acggaccttt catcccttga aagcacccct ggatgctcct
     4861 tgttcctatt caagtatgta acaaatttca tcaaaacacc tccagaattt tcttgattat
     4921 atcattgacc tgattcatat tgaaaaaggg cgtaataagc tacgccccct gtttttccat
//
LOCUS       DASRQF010000229         4581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_379496, whole genome shotgun sequence.
ACCESSION   DASRQF010000229 DASRQF010000000
VERSION     DASRQF010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379496"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VFD79_06080"
     CDS             <1..744
                     /locus_tag="VFD79_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HZH93654.1"
                     /translation="SEEGRLIRKAFIAQTPDYVFLDGDYSQIELRLLAHISSDPKMLD
                     AFINNEDIHTKTAAEVFGYPKDEVTSELRYKAKAINFSIIYGISDFSLSKDIGISRKE
                     ARKYIDNYFENYTGVRSYMDDAVAKGKENGYVETLLNRRRYVPELASRNYNIRSFGER
                     VAMNMPIQGSAADIIKMAMVNVFKVLKDKKLKSRLILTIHDELIIEAHRDEVQDVKQM
                     MKETMENAIKLQVPLRVDILEGDSWYDTK"
     gene            731..1348
                     /gene="coaE"
                     /locus_tag="VFD79_06085"
     CDS             731..1348
                     /gene="coaE"
                     /locus_tag="VFD79_06085"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HZH93655.1"
                     /translation="MIQNNVKVIGITGGIATGKSTVSQIIRNEGFQVIDSDSIAREVV
                     ERGSKGLALIINEFGKDLLMEDGNLDRERLGEIIFNDDNKRETLNSLLHPLIRESMVS
                     RISQAAGFNKVVFVDIPLLFESRETIEESGVKLDEVWLVYTEEAIQLSRLMNRNNYKY
                     EEALARIRSQMDIEKKRLLSDRLIDNSGSVEDTRKQVLDLLNEYR"
     gene            1358..1981
                     /locus_tag="VFD79_06090"
     CDS             1358..1981
                     /locus_tag="VFD79_06090"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HZH93656.1"
                     /translation="MKKIFISITVALILAALAVSGVALTYPVVYKESVVKYSEEYNLD
                     PMLVMAVIKTESNFDKDAISHKGARGLMQIGESTGRWASEILKLEEYTGDLLYDPETN
                     IRIGAWYLRELLDQYDHEYVALAAYNAGSGNVSEWLSNQEYSNDGENLQNIPFPETEE
                     YVRRVQRNKRVYELVYGSSLLSGRDTFFDGLVISTRNWLKNTIKGLR"
     gene            1987..3633
                     /locus_tag="VFD79_06095"
     CDS             1987..3633
                     /locus_tag="VFD79_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HZH93657.1"
                     /translation="MRKIAIFIIIALLLFSVSGCGLEPDSTDIGEIQEGVDYFIFENA
                     GISLPLTSFKTLNPLYMDNLSYYHFSKLIFEGLFEYDENLQPVPRLASTYTISEDGRT
                     VEVYLRNDILWHNGESLDSDDVVFTVQTMKKAGEESLYGKLVRSGASPGNLNIMSARA
                     LSENRVEISFSEPTGNILDMLTFPIISKGLGSGALAVENYIPIGTGPYMFVEHVKFKE
                     VHLESNPQYREGEPQISYITGKIYDDRGLVLTAFETGKLDMAPSIGIDWDKYEHNERI
                     SIHEYVSGDQELLAFNSLDERFASDGSEQLKQAILYAIDRQSIIDKVYLQHGTQVDTP
                     VHPASYLSSSETDMYGYNIGSAEGILNGGGYIDTDGDGIREDSEGNPLNIEMLVNPYS
                     DLKLKTSEMIKSNLKEVGISVEIVYPPGKTSQEAMENMGNMLTEGDYQTAILQWEQST
                     IPSFESFLGSSRPENLARFSDEKMDQLLSEMRVAWDENTKIDIYRDFQEYYVEKVPYG
                     SLLFRNKALLVDSGISGPLQPTYYNLYNGLESCYLTMSTN"
     gene            3720..3806
                     /locus_tag="VFD79_06100"
     tRNA            3720..3806
                     /locus_tag="VFD79_06100"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3754..3756,aa:Leu,seq:gag)
     gene            3961..>4581
                     /locus_tag="VFD79_06105"
     CDS             3961..>4581
                     /locus_tag="VFD79_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HZH93658.1"
                     /translation="MNQGKLTFKGGVHVPEYKELAESKSIEVAESPQFAYIPLHQHIG
                     APCDPVVNVGDRVTIGQKIGESGAFVSGTVHASIAGTVKEIKMMYTPTGGKAKCIVIE
                     NDGTDEMHESIKGKSLGDLSPQEIIGLVKESGITGMGGASFPAHVKFSIPEDKKIDTI
                     ILNGAECEPYLTGDHRLMLEEPEAIVFGLKAIKKALGVEKAYIGIEN"
BASE COUNT         1516 a          790 c         1086 g         1189 t
ORIGIN      
        1 agtgaagaag gaagacttat tcggaaggca tttattgcac agactcccga ctatgttttt
       61 ctggatggag actactctca aatagagtta aggctactgg cccatatttc ctctgatcca
      121 aaaatgcttg atgcattcat aaataacgaa gacatacaca ccaaaactgc agccgaggtg
      181 ttcggatatc caaaggatga ggttacatca gagctcagat acaaggcaaa ggctataaac
      241 ttcagtatta tatatggaat ttcagacttt agtttatcca aggatatagg aatcagtcgt
      301 aaggaagcaa ggaaatatat agacaattat tttgagaatt acacaggggt aaggtcctac
      361 atggacgatg cagttgccaa gggtaaggaa aacggatatg ttgagaccct gctgaaccgt
      421 cgtagatatg tacctgaatt ggcgtccagg aactataaca taaggtcatt cggagaaagg
      481 gtagcaatga acatgcccat acagggaagt gcagctgaca taataaaaat ggccatggtc
      541 aatgtgttta aggttctaaa agacaagaag ctaaagtcca gattgatttt gacaatccac
      601 gatgaattga ttattgaagc ccacagggat gaggttcagg atgtcaagca aatgatgaag
      661 gaaacaatgg aaaatgcaat taagctgcag gttcctctta gggtggacat actggagggt
      721 gatagctggt atgatacaaa ataatgtaaa ggttattgga atcaccggtg gaatcgcaac
      781 aggtaaaagc acagtcagcc aaatcataag aaatgaaggc tttcaggtta tagattcaga
      841 ctctattgcc agggaggttg tcgaaagggg aagcaagggc ctggcattga taataaatga
      901 atttggcaag gatttgctca tggaagatgg aaatcttgat agggaaaggc ttggggaaat
      961 aatatttaat gatgacaata agagagagac tctcaacagc ctcctccacc cactgatcag
     1021 ggaatccatg gtatcgagga tatcccaggc cgcagggttt aacaaagtgg tctttgttga
     1081 tattcccctg ctgtttgaat cccgggaaac catagaggaa tctggtgtta agctagacga
     1141 ggtatggctg gtttatactg aggaagcaat tcaattaagc agacttatga acaggaacaa
     1201 ttacaagtat gaagaagctt tagccaggat aaggtctcaa atggacattg aaaaaaagag
     1261 gcttctgtca gataggctta tcgacaattc gggaagtgtt gaagatacaa gaaagcaggt
     1321 cttggacctg ctgaatgaat atagatagtg gtgagttatg aaaaaaatat ttataagtat
     1381 aacggttgcc ttgattttag ctgcccttgc agtatcagga gtggccctga catatccagt
     1441 ggtctacaag gagtctgtgg tcaaatattc agaggagtac aatctggacc cgatgcttgt
     1501 catggcagtt attaagacag agagtaactt tgacaaggat gccatctccc acaagggtgc
     1561 aagagggtta atgcaaattg gcgaatcaac aggtcgttgg gcatctgaaa tcttaaagct
     1621 ggaggaatat actggtgatc tcctatatga tcctgaaaca aatattcgaa taggggcctg
     1681 gtacctaagg gagctgttgg atcaatacga ccatgagtat gtggctttgg ctgcctacaa
     1741 cgctggaagt ggaaatgttt cagaatggtt gtcaaaccag gaatacagca atgatggtga
     1801 aaaccttcag aatataccat ttcccgaaac tgaagagtat gttaggaggg tccagcgaaa
     1861 taaacgggtt tatgagcttg tatacgggag cagcctatta agcgggagag atactttttt
     1921 tgatggactt gtgattagta cgaggaattg gttaaaaaat acaattaaag ggttaaggta
     1981 gatataatga ggaaaatagc catttttatc atcatagcat tacttttatt ttctgtttcc
     2041 ggttgtggat tggaaccgga ttcaacagat attggagaaa ttcaggaagg tgttgactac
     2101 ttcatttttg aaaatgcagg tattagtctt cccttgacta gcttcaaaac actgaatccc
     2161 ctatacatgg acaatctcag ttactaccat ttcagcaagc tcatatttga aggcctattt
     2221 gaatatgatg aaaatctcca acctgtacca agattggcca gtacctacac cataagtgaa
     2281 gatggcagga cagtggaggt atacctgaga aatgacatat tgtggcataa tggagaatcc
     2341 cttgactcag atgatgttgt gttcacagta caaaccatga aaaaggcggg ggaggagagc
     2401 ctctatggaa agctggttcg aagcggtgcc tctccaggca acctgaacat catgtcagcc
     2461 agggcattgt cggagaacag ggtggaaatc agctttagcg aacctacagg taacatcctt
     2521 gacatgctta catttccaat aatctccaaa ggtttgggaa gtggagcatt ggctgtggaa
     2581 aactatattc ccattggaac tggaccctat atgtttgtgg aacatgtaaa attcaaggag
     2641 gtccaccttg aatcaaatcc tcaatacagg gaaggagagc cccaaatatc atatataaca
     2701 ggaaaaattt atgatgacag aggcttggta ctcactgcct ttgaaactgg aaaactggat
     2761 atggctcctt ccattggaat tgattgggac aagtacgaac acaatgaaag aatttcaatc
     2821 catgagtacg tttctgggga ccaggagctt ctggccttta acagtctaga tgaaaggttt
     2881 gcttctgatg gctctgaaca attgaagcag gcaatcctat atgccatcga taggcaatcc
     2941 ataatcgaca aggtgtattt gcagcatgga acccaggtag acacaccggt acatccagct
     3001 tcatatttgt catcctcaga gacggacatg tatggataca acattggaag tgctgaagga
     3061 atcttgaatg gtggtggcta tattgacacc gatggagatg gaattcgaga ggacagtgaa
     3121 ggaaatccat taaatattga aatgttggta aatccctatt cggatctaaa actaaaaaca
     3181 tcagaaatga tcaagagcaa tctaaaggaa gttggaattt cagttgagat agtctatcct
     3241 ccagggaaga cttcccagga agcaatggag aatatgggga atatgttaac tgagggtgat
     3301 taccaaacgg caattctgca atgggagcaa tccactattc catcatttga atcattccta
     3361 ggatcatcca ggccagaaaa tctagcgcgt ttttccgatg agaaaatgga ccaattactt
     3421 tctgaaatga gagttgcctg ggatgaaaat acgaaaatag acatctatag agattttcag
     3481 gaatactatg ttgagaaggt tccatatgga agcctgttgt ttagaaacaa ggctctgctg
     3541 gttgattcag gcatatctgg gccccttcaa ccaacatact acaatctata caatggattg
     3601 gaaagctgct atctcacaat gagtacaaat taggattgaa atttgagcca aaagatatta
     3661 taatgtacta gtaacatttc caatgaaaca gtcactcgac tgtttcatca ttatattgtg
     3721 cgagtgtgct ggaatcggca gacaggcatg tttgaggggc atgtgccaga attggcgtat
     3781 gggttcaagt cccatcactc gcaccatatc aatttcaatc tcccaaatta acaaatatat
     3841 ataccctgat ttatatacat taataacaat taatgttata atatatccat aagggcaatt
     3901 tttgtaaaaa aatttaacaa aaattgctaa aattatatca agtaaagagg tggaataaga
     3961 ttgaaccaag gtaagttgac tttcaaaggt ggcgtacatg ttcctgaata taaggagctg
     4021 gcagagagca agtcaataga agttgctgag agtcctcaat ttgcttacat acctttgcac
     4081 caacacattg gtgcaccatg tgatccagta gttaatgtag gtgacagggt tacaatagga
     4141 caaaaaattg gggaatcagg agcttttgta tcaggtactg ttcatgcgag tatagctggt
     4201 acggtgaagg aaatcaagat gatgtataca cccactggtg gaaaagccaa atgcatagtc
     4261 attgaaaacg atggcactga cgaaatgcat gaatccatta aagggaaaag cctaggcgac
     4321 ctttccccac aagagataat tggtcttgtt aaggaaagtg gtatcacggg aatgggtgga
     4381 gcaagttttc ctgctcatgt aaaattctca attccagagg ataagaagat tgacaccata
     4441 atactcaatg gagctgagtg tgagccctac ttgacaggtg accacagatt gatgctagaa
     4501 gaaccggaag caatagtatt cggactcaag gctatcaaga aggctttagg tgttgaaaaa
     4561 gcgtacatag gaatcgaaaa c
//
LOCUS       DASRQF010000230         2602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1657183, whole genome shotgun sequence.
ACCESSION   DASRQF010000230 DASRQF010000000
VERSION     DASRQF010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1657183"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="VFD79_06110"
     CDS             complement(<1..1434)
                     /locus_tag="VFD79_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93659.1"
                     /translation="MKMKIRVKTLLLGMIVLAVGYLWVIPQSTLAIAKYLDGKDSPRA
                     GLFYERHIKMDDSIQGRLDYAQSLVKYFGKFTIYQQGWGGGEETSREDLIRAEELLQE
                     ILQMEPSPREEKLYMDAYRMRMDIAIAKGDVDGLRQLIYLGENHKEIMMDEASLYKAY
                     LHFTNREFEEAKIIVDRLQQEGLDDPRQEILLAEIHLANGDAKGARDLYKKLQARDWR
                     SLKDTYFASPTFGHRNFWLDESRSLLEGNNRIKGRVTFEGEPMAFVEIYILSADGGVR
                     IGGESFVAITDEKGYFETLPLSDGIYDVGLGLDGSLLTDKVFQKSETGYVQVPGDDFI
                     EYTFRDTFQVYSPQPGSVVEDDLFTVSWEEVPEADYYTVEPVIFSNPLERSGGSFRSP
                     IEDIGGEGRFRGTSADFRISDFRNQTGGMTFEGEEWLLGSGAVLGKFLPGGEYPISVN
                     AYDKNNQLITSSFPMRTYYDKIPSIVVE"
     gene            complement(1431..1745)
                     /locus_tag="VFD79_06115"
     CDS             complement(1431..1745)
                     /locus_tag="VFD79_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93660.1"
                     /translation="MNRDDFIKDDIKKIFDDEASEIKFTDEMMENVLDQTTRSGKGKI
                     RRILNYELQIPLIPAAVAVAAILAVSILPEGILEKQYRFTAIEMAGSQVMVQEDKEVT
                     RR"
     gene            complement(1738..2271)
                     /locus_tag="VFD79_06120"
     CDS             complement(1738..2271)
                     /locus_tag="VFD79_06120"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZH93661.1"
                     /translation="MTENQMINGLIKGQEDAFRQMMQLYGNKLLGTCYLILRDREEAE
                     DVIQETFVRVFRNIRTFNGKSSLYTWIYRIAINLSRDRLRKKTYDMSLNEELVGDADT
                     LDILEHRMDMEELKISLAELSPIYREVLVLFYYQDLSISEISDLLKEKEGTIKSRLSR
                     ARKILAKEMQKGGSEDE"
     gene            complement(2371..2601)
                     /locus_tag="VFD79_06125"
     CDS             complement(2371..2601)
                     /locus_tag="VFD79_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005957132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HZH93662.1"
                     /translation="MLYYKVGDQVTLKKGHPCGENHWEVLRTGVDIKLKCMGCERQIW
                     LTRIEFEKRVRKILVEDKWISIVHHKPEGSAE"
BASE COUNT          612 a          709 c          462 g          819 t
ORIGIN      
        1 ctccacaact atgcttggaa tcttgtcgta ataggttctc atgggaaagc tgcttgtaat
       61 gagttggttg tttttgtcat atgcatttac gcttatgggg tactcccctc ctggtaggaa
      121 cttgccaagt acagcacccg agcccagaag ccattcctct ccctcaaagg tcatcccacc
      181 ggtttggttt ctgaagtctg atatcctaaa gtctgcactt gttcccctaa accttccctc
      241 accaccaata tcttcaatgg gactcctaaa agaccctcct gatctttcca ggggatttga
      301 aaatattaca ggctcaactg tgtagtaatc cgcctccgga acctcttccc atgacactgt
      361 gaacaggtcg tcttcaacaa ctgaaccagg ctgaggtgaa tagacttgga aggtgtctct
      421 gaaggtatac tcaataaagt catctccagg gacctgaacg tatcctgtct cacttttctg
      481 gaagaccttg tcagtcaata ggcttccatc cagtccaagg cctacatcat atattccatc
      541 cgaaagagga agggtttcaa aatacccctt ctcatcggtg attgctacaa aggattctcc
      601 tccgattctc acacctccat cagcactaag gatatagatt tccacaaatg ccatgggctc
      661 tccctcaaag gtgacccttc ccttgatcct gttgtttccc tctagaaggc tccgggattc
      721 atccagccag aaatttctgt gaccaaatgt tggagatgca aagtaagtgt cctttaaact
      781 tctccaatcc cttgcctgga gttttttgta gaggtctctt gctcccttag catcaccatt
      841 ggcaagatgg atttcagcca ggagaatctc ctgtcttgga tcatccagtc cttcctgttg
      901 gagtctatcc acaataattt tggcttcttc aaattccctg ttggtaaaat gcagataggc
      961 cttgtagagc gatgcctcat ccatcatgat ttccttatgg ttttcaccca agtatatcag
     1021 ctgtcttaat ccatccacat ctccttttgc gatggctatg tccattctca tcctatatgc
     1081 atccatgtat aacttttctt ctcttggtga tggctccatt tggaggattt cctggagcaa
     1141 ctcctctgcc ctgatcaaat cctcccggct ggtttcctct cctccgcccc agccctgctg
     1201 gtagattgtg aacttcccaa agtactttac gagactttgt gcatagtcaa gtcttccctg
     1261 gatggaatcg tccatcttga tatgtctttc atagaacaga cccgcccttg gggaatcctt
     1321 gccgtccagg tattttgcta ttgccagtgt agattgggga atcacccaga ggtagcccac
     1381 agccaagaca atcatcccta aaaggagtgt tttcaccctt attttcatct tcatcttctt
     1441 gtcacctctt tatcctcctg aaccataacc tgggacccgg ccatttcaat tgcagtaaac
     1501 ctgtactgct tctccaagat tccctcaggc aggattgaga ctgcaaggat tgccgccaca
     1561 gccacagctg caggtatcaa gggtatctga agctcatagt ttaggatcct tctaattttt
     1621 cctttccctg accgggttgt ctggtccagg acattttcca tcatttcgtc cgtaaactta
     1681 atctctgatg cttcatcatc aaaaattttc tttatatcat cctttataaa gtcgtctcta
     1741 ttcatcttca gaacctccct tctgcatttc tttggccaga attttccttg cccttgacaa
     1801 tctgctcttt atggttcctt ccttttcctt caaaaggtct gaaatctctg atatgctaag
     1861 gtcctgatag tagaaaagta ccaggacttc cctgtaaatt ggacttagtt ctgcaagact
     1921 gatcttaagc tcctccatat ccatcctgtg ttcaagtatg tccagggtat ctgcatcgcc
     1981 aacaagctct tcattcaggc tcatatcgta tgtctttttt cttagcctgt cccttgaaag
     2041 gttaattgca attctataga tccaggtgta gaggctgctt ttcccattga aggtccggat
     2101 gttcctgaac acccttacaa aggtctcttg gataacatcc tcggcttcct ccctgtctct
     2161 gagtatcagg taacaggtcc cgagaagctt gtttccatac agctgcatca tttgtctaaa
     2221 ggcatcctcc tgccccttta tcagtccatt gatcatctgg ttttctgtca ccttcaaccc
     2281 tccttacact ttagacggat tttcatcaaa aaggttgcat caaaaaacaa aaaagagaga
     2341 aatattctcc ctttgattat acaatgccag ctactcagca ctcccctccg gcttatgatg
     2401 gacaatgctt atccacttat cctccacaag aatctttctg acccttttct caaactcgat
     2461 cctggtaagc caaatctgcc tttcacaccc catgcactta agtttgatat caactcctgt
     2521 ccttaatacc tcccaatggt tctccccaca tgggtgcccc ttcttcaatg ttacctggtc
     2581 tccaaccttg tagtatagca tt
//
LOCUS       DASRQF010000231         2024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1248390, whole genome shotgun sequence.
ACCESSION   DASRQF010000231 DASRQF010000000
VERSION     DASRQF010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248390"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1453)
                     /locus_tag="VFD79_06130"
     CDS             complement(128..1453)
                     /locus_tag="VFD79_06130"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZH93663.1"
                     /translation="MKEFKNNSKFWLLALIIAMLFSIYFIIYIPMVNNLRAMTVENMH
                     MAAHYKMTILERDINQAMVGARGISTRSLLKESLYKYSNGEMELAELKEISQNRFTDG
                     IDTLEGLESAIRVTDGEIIASYNGEIDYDEELNKIEEITYSISPGSSKLKIVSPIQSE
                     EKLLGHDIFLFDLIPTIEKLDMENLKGILITEEERKALVSNTGFFEVEDNHLIAEDDQ
                     FIYHIERISNTDSYLYILADKGLIYKSVSQVTKANLINFIIIGLTFVFTSTIITTKLA
                     QGKIDELEVGRDKYRSQYLIDPLTGAFTRRALSEGREEKLISEDFKGIAVMMDLTDFK
                     RINDSHGHKEGDRVLVKLVEIMFESLREDDLVIRFGGDEFLVLLHDCEMEEAKSIINR
                     IRQNFKKSSEFKYNVDFDYGMEIINSYGELNDSIAKSDERMYRNKGRGK"
     gene            complement(1473..>2024)
                     /locus_tag="VFD79_06135"
     CDS             complement(1473..>2024)
                     /locus_tag="VFD79_06135"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZH93664.1"
                     /translation="WVIDTACSQSKDWYQKDLRDLSISINISGEMLGRREWIEKIQRY
                     ISDCSSHGRIVFEITETAIISDLEVSLESLKQIHDMGIRLALDDFGTGFSSLTYLQKL
                     PVDIIKLDKDFIWEIGKDEGKEFIIKAVIDLAHNLGMTVVAEGVETREQLEFLKGADC
                     DYAQGYYFYKPTTAGAIEGLLRN"
BASE COUNT          548 a          492 c          291 g          693 t
ORIGIN      
        1 atggaatacc attatcttca agaatagctg ttatcatgtt catttcaaat tcactttcta
       61 ttgtccttaa aacaactaaa tccaagttat ctgacataat tacctcctta ttccatcctt
      121 gaaaagacta cttaccccta cccttatttc tatacatcct ttcatcggac tttgcaatac
      181 tatcattgag ttcaccatag ctgtttatta tctccatacc atagtcaaag tcgacgttat
      241 acttgaattc tgaggacttc ttgaaatttt gtctgatcct atttattatt gattttgcct
      301 cttccatttc gcaatcatgg agcaatacca agaactcatc ccctccaaac ctaatgacaa
      361 gatcatcctc cctgagtgat tcaaacatta tttcaaccaa tttcacaaga accctatccc
      421 cttccttgtg cccatggcta tcattaatcc ttttgaagtc cgtcaagtcc atcatgactg
      481 caattccctt aaaatcctct gagataagct tttcttctct cccctctgac aatgctcttc
      541 ttgtaaaggc ccctgtaagt ggatctatta gatattgact tctatactta tccctgccca
      601 cttcaagctc atctatctta ccctgtgcca attttgtagt aattatcgtc gatgtgaaca
      661 caaaggtcag gccaattatt atgaagttta taaggttggc ctttgtcacc tggctaactg
      721 atttatagat taatccttta tcagctaata tatacaggta tgagtccgtg tttgaaatcc
      781 tttctatgtg gtaaataaat tggtcatctt ccgcaatcaa atgattgtct tccacctcaa
      841 aaaaacctgt attggacacc aaggcctttc tctcttcctc tgtgataagt atccccttaa
      901 gattctccat atccagcttt tcaattgttg gaatcaaatc aaacaagaaa atgtcatggc
      961 ctaaaagctt ttcttcactt tgaattggag aaacaatctt aagcttgctg cttccaggac
     1021 ttatgctata tgttatttct tctatcttgt ttaattcctc atcataatca atttcgccat
     1081 tatatgaggc gatgatttcc ccgtcagtga ccctgattgc actttccaaa ccctcaaggg
     1141 tatctatgcc atctgtaaat ctgttttggg aaatctcctt aagttcagcc agctccattt
     1201 ctccatttga gtacttatac agactttcct tcaataaact tctggtgctt atccctcttg
     1261 ctccaaccat ggcctgattg atatccctct ccagaatggt cattttgtag tgggcagcca
     1321 tatgcatgtt ttcaactgtc atagctctca ggttattaac cataggtata taaataataa
     1381 agtatattga gaacagcata gcaattatca aggccaaaag ccagaatttg gagttgtttt
     1441 tgaattcttt cataagactc aatcccctgt atctagtttc ttaacaatcc ttctatagct
     1501 ccagcagttg taggcttgta gaaatagtag ccctgtgcat aatcgcagtc agctcccttt
     1561 aggaactcca gttgttccct tgtctccaca ccctctgcca ccactgtcat tccaaggtta
     1621 tgagccagat cgataacagc ctttattatg aattccttgc cttcatcttt cccaatttcc
     1681 cagatgaaat ccttgtccag cttgatgatg tcaaccggaa gcttctggag gtatgtcaag
     1741 gacgaaaagc cagttccaaa atcgtccaag gcaagtctta ttcccatatc atgaatttgt
     1801 ttcaatgatt ccagactaac ctccaaatct gatattatgg cagtttcagt tatctcaaat
     1861 acaattcttc catggctact acaatcagat atataccttt gaatcttttc tatccattct
     1921 ctcctcccaa gcatttcccc tgagatgttt attgatatgg acaagtccct gaggtccttc
     1981 tggtaccaat ccttgctttg actgcaagct gtatcaatta ccca
//
LOCUS       DASRQF010000232         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1502747, whole genome shotgun sequence.
ACCESSION   DASRQF010000232 DASRQF010000000
VERSION     DASRQF010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502747"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VFD79_06140"
     CDS             <1..549
                     /locus_tag="VFD79_06140"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HZH93665.1"
                     /translation="ILDGVVFTGGHDVWPQRFGEEPVKQVVEITHERDQLELVLFEKA
                     YGAGVPILGICRGLQLVNIALGGTLYQDIYSQVEGVGGHSFGYNPEEVYHSIQIEEGS
                     IMKEIYNRETVHVNSEHHQAIKDLGRDLKVTSRAPDGIIESIESTNDSFILAFQYHPE
                     AIAPKYPEHQKIFNYFVEKCRA"
     gene            complement(631..1764)
                     /locus_tag="VFD79_06145"
     CDS             complement(631..1764)
                     /locus_tag="VFD79_06145"
                     /inference="COORDINATES: protein motif:HMM:NF019450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HZH93666.1"
                     /translation="MYSLKGKSVLRKTLVMLMVLALLLPSAAMAEELSFVEVGYDGAY
                     AVDGVHMHLNSEVDVQNNSYDFSVTNAVETFDASEDDYYLRPGINVDGSTVVTLLKDN
                     ALEFGVYKLTDVGDNQFELDSNNVLPIDGQISFYGKTDAVDEEGNPVYASMTIDITDM
                     ENHEMDMYYQVKGNTVSITEPGHYFVNLRYEAAAGAAQAVLVVQETQEATAEEEPVEE
                     EPIAEEPVAEEPTLDPLVNALPTSAKVLVNGEEVSFNAYNINGNNYFKLRDLATAVSG
                     SDKQFQVEWDSEVRAINLISGQAYTSVGGEMTSGDGEAKEGTLNTSKIYKDGEEIQLS
                     AYTINDNNFFKLRDVAEVFDIGVTWDGETKTIGIDTSVGYTAE"
     gene            1999..>2086
                     /locus_tag="VFD79_06150"
     CDS             1999..>2086
                     /locus_tag="VFD79_06150"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZH93667.1"
                     /translation="MEYTIKKLAKLAGVSTRTLRYYHEIGLLK"
BASE COUNT          596 a          443 c          419 g          628 t
ORIGIN      
        1 atcctggatg gtgtagtatt cacaggtggt catgatgttt ggccacaaag gtttggtgaa
       61 gagcctgtga agcaggtggt tgagataacc catgaaaggg accagcttga gcttgtactt
      121 tttgaaaagg catacggggc aggagtaccc attcttggga tttgcagagg cctgcagctt
      181 gtgaacatcg ccctgggagg gaccctctac caggatatct acagtcaggt ggagggtgta
      241 ggcggacact catttggcta taatccggag gaagtctacc actcaataca aattgaagag
      301 ggttcaataa tgaaggagat atacaacagg gagacagtcc atgtgaattc cgagcaccac
      361 caggcgataa aggatttggg aagggacctg aaggtcacat caagggcacc ggatggaata
      421 attgaatcca tcgaatctac aaatgatagt tttatccttg catttcagta ccatccggag
      481 gcaattgcgc ccaagtaccc tgaacatcag aagatcttca attattttgt tgaaaaatgt
      541 agagcttaaa taatcattaa tcattaacaa acccttcttg gcaataggcc aggaagggtt
      601 tgtgtatgtt ctattttaag ctctgtttga ctattctgca gtgtacccca ctgatgtatc
      661 aattccaatt gttttggttt ctccatccca ggttacccca atatcgaaaa cttctgcaac
      721 atctctcaat ttgaaaaagt tgttgtcatt gatggtgtag gctgaaagtt gaatttcctc
      781 accgtctttg tatatctttg atgtgttcaa tgtaccttcc tttgcttctc cgtctcctga
      841 agtcatttct cctccaactg atgtgtatgc ttgtcctgaa atcagattga ttgctctaac
      901 ctcgctatcc cactcaacct ggaactgttt atctgaacca ctcactgcag ttgcaagatc
      961 tctaagctta aagtagttgt ttccattgat attgtaagca ttgaaggaaa cctcctcacc
     1021 gttaacaaga accttggcac ttgtaggcaa tgcattaaca agaggatcta gtgtgggctc
     1081 ttcagcaact ggttcttcag caataggctc ttcctcgaca ggctcctctt cagcagttgc
     1141 ctcctgggtt tcctgaacaa ctaaaactgc ttgtgctgcc cctgcagcag cttcgtatct
     1201 aaggttaaca aaataatgtc caggctcggt gattgaaact gtatttccct ttacctggta
     1261 gtacatatcc atctcgtgat tttccatgtc agtaatatca attgtcatgg aagcatatac
     1321 tggatttcct tcttcgtcca cagcatcagt tttcccgtag aaggaaatct gtccatctat
     1381 cggtaataca ttgtttgagt caagttcaaa ctggttgtct ccaacatctg taagcttgta
     1441 aaccccgaac tcaagggcat tgtcctttag caaggttaca actgtcgatc catccacatt
     1501 aattcctgga cgcaaatagt aatcatcttc agatgcatca aatgtttcaa ctgcgtttgt
     1561 cactgaaaaa tcataggagt tgttttgaac atctacttcc gaattaagat gcatatgtac
     1621 cccgtccact gcataggctc catcataccc cacttccaca aatgataatt cctctgccat
     1681 tgctgctgaa ggtaggagca atgccaatac cataagcatt acaagtgttt ttcttaatac
     1741 agattttcct tttagactgt acataatatg cctcctctaa attattagaa ttttttgaaa
     1801 cccaaaaaat attatagcat attttccacg taatttacat ataatttcaa caaacaggaa
     1861 ataatgtaag aggaaagggt tgaagtcggt ccgttgtgat tttcgatgaa taccaataaa
     1921 tttttagaat gagatttcca gaaatccctt gacctttacg ctacgtaatg gtttatatta
     1981 aatgcaggag gtaatattat ggaatacaca atcaaaaaac tggccaagct tgcaggggta
     2041 agcacaagaa ccctgaggta ttaccatgag atagggcttt tgaagc
//
LOCUS       DASRQF010000233         2252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_222872, whole genome shotgun sequence.
ACCESSION   DASRQF010000233 DASRQF010000000
VERSION     DASRQF010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222872"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VFD79_06155"
     CDS             complement(<1..307)
                     /locus_tag="VFD79_06155"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZH93668.1"
                     /translation="MFNGHTDTVLPYNMTIDPFAATLSDGKIYGRGSVDMKGALASFM
                     VSMVAMKRSGYVPRGDVIFTAVIGEEGKSEGTEYIVKSDITADGAIVGEPSDYEYAIG
                     "
     gene            complement(552..>2252)
                     /locus_tag="VFD79_06160"
     CDS             complement(552..>2252)
                     /locus_tag="VFD79_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HZH93669.1"
                     /translation="PSWMHVVIIYSMLIALLSSTLPGTSDYIRWAWLNDKVNSAFPIV
                     QELRSGEGARRVSGDALAFDFSTTGFQDDSSLLGGPVEIDDTLVMSIRGDRVNYLRGN
                     VRHTYTGTGWMEDPGFFELVQEGSDFSGLSDEEKALYYREAGFSITNIRLSSTTVFSP
                     LQPYSFTFEDSLGLLVNGDFSLSYSDGVYAGQTYFVRAYIPRAYGIQVERGRDKSLDD
                     LMNPNPYLDLPDTISDRTIQLANAITDGLDSGIDKALAMESYLRESYEYNLDVPHVPE
                     NTDFVDYFLFQEKEGYCTYFATSLAVLLRIEGIPTRYVEGFTIGPETDEGIQEVRNSN
                     AHAWVEAYIEPVGWITLEATPAYNPPARLLDYNPQLVEDVESDQVDGRSRDPESADGR
                     GEDSDDVTGDTPGTQGADKENPSWTSSSFALFTLLVITLVVMGRFLRGIYHVRKDKQS
                     YQGLDNRGKVLYLYHEMTKHVERLGYGSNPGETHFEYSNRISHKFHDFGKFGFKQATQ
                     LFVKSKYGDYIPSLDEISIMEDYLKKLDLKLKRHFGSIPYHLRKAVKLFNYKGIYERK
                     "
BASE COUNT          614 a          540 c          449 g          649 t
ORIGIN      
        1 ggcctatggc atactcgtag tctgagggct cacccacaat tgcaccatca gctgtgatat
       61 ctgactttac tatatattca gttccttcac ttttaccctc ttcgccgatg actgcagtga
      121 agatgacatc acctcttgga acatagccac ttcttttcat tgcaaccatg gataccataa
      181 aggatgcaag ggcacccttc atgtccacag atccccttcc atagattttc ccatcagaaa
      241 gggttgctgc aaagggatca atggtcatat tgtaaggcaa gaccgtatcc gtgtgtccat
      301 tgaacattag tgatttccca tccccctttc ctttacttgt agctactaca ttgggtcttc
      361 cgtccacaac agggtgaagc tccgaatgaa tcccattatc aagaagataa ttgtgaataa
      421 attctgcaac atctttttca aatccaggag tatcctaatg gctgggtatc ctgaccaggt
      481 cttgtgtaag gcttatcaac tcagcctcag taaaatattt tcttgggtgg aagttcattt
      541 ctgtcaaaat atcacttcct ttcataaata cctttataat tgaaaagttt gacggctttt
      601 cttaaatggt agggtattga accaaaatgc ctcttaagct taaggtcaag ctttttaagg
      661 tagtcctcca tgatgcttat ctcatccagg ctgggaatat aatctccata cttggacttt
      721 acaaacaact gggttgcctg cttaaaacca aatttaccaa agtcgtgaaa cttgtgggaa
      781 atcctattgg aatactcaaa atgagtttct cctggattgc tcccatatcc caacctttca
      841 acatgcttgg tcatctcatg ataaaggtat aggaccttgc ccctgttgtc aaggccttgg
      901 taggactgct tatctttcct aacatggtat attcccctta agaatctacc catcaccact
      961 aacgttatca caagcagagt aaataatgca aatgatgagg atgtccatga gggattctcc
     1021 ttgtctgcac cctgtgtacc aggtgtatcc ccagttacat catctgaatc ttcccctctt
     1081 ccatcagcag actcaggatc tctacttctg ccatcaactt ggtcagattc aacatcttca
     1141 acaagctggg gattataatc caagagcctg gcaggagggt tgtaggcagg tgttgcttcc
     1201 agtgtaatcc acccaaccgg ctctatatag gcctctaccc atgcgtgggc gttgctgttt
     1261 ctgacctctt gtataccctc atcggtttcc ggaccaatag taaaaccttc cacatacctg
     1321 gttggaatac cttcaattct caatagaaca gccagggaag tcgcaaaata agtgcaatag
     1381 ccctccttct cctggaagag gaaatagtca acaaagtcag tgttttcagg gacatggggt
     1441 acatccagat tatattcata tgactccctt aggtagcttt ccattgcaag cgccttatca
     1501 atacctgaat ccagcccatc tgtaattgca tttgcaagtt ggatagtcct gtctgaaatt
     1561 gtgtcgggaa ggtctaaata agggttggga ttcattaggt catctaggct tttatccctt
     1621 cccctttcaa cctggattcc ataggcacgt gggatgtagg ccctgacgaa ataggtttgg
     1681 cctgcataga ctccgtctga ataagaaagt gagaagtcac catttacaag caatcccaaa
     1741 ctgtcttcga aagtgaatga gtagggttgg agtggactaa atacagttgt tgaggataat
     1801 ctaatatttg tgattgagaa accagcctcc ctatagtaaa gtgctttttc ctcatctgac
     1861 agacctgaaa aatcagaccc ctcctgtacc agttcaaaaa acccagggtc ttccatccaa
     1921 cctgtcccag tatatgtgtg cctgacattg ccacgaaggt aatttaccct gtcgccacgg
     1981 atactcataa ctagagtatc gtcaatctca accgggccac ccagaaggga ggagtcatcc
     2041 tgaaatcctg ttgtagaaaa gtcaaaagct agggcatcac cagaaaccct tctggcacct
     2101 tctcctgaac gcaattcctg tacgatgggg aatgccgagt ttaccttgtc attcagccat
     2161 gcccatctta tatagtctga tgttccaggt aaggtggatg aaaggagtgc gattaacata
     2221 ctgtagatta tgactacatg catccaggac gg
//
LOCUS       DASRQF010000234         3120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_586898, whole genome shotgun sequence.
ACCESSION   DASRQF010000234 DASRQF010000000
VERSION     DASRQF010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586898"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VFD79_06165"
     CDS             <1..406
                     /locus_tag="VFD79_06165"
                     /inference="COORDINATES: protein motif:HMM:NF016178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HZH93670.1"
                     /translation="VGTSLTYESWYFCDNEEDANELAQLVLEGTKRATASLYKSYEFE
                     KEELPKEGSHIVVTDWNGTAKCIIRTNKVSILPFKDITEDHARIEGEGDKSLKYWKDG
                     HTRFFTRECEVMGIEFNQDMLVVFEEFQVVYP"
     gene            complement(458..856)
                     /locus_tag="VFD79_06170"
     CDS             complement(458..856)
                     /locus_tag="VFD79_06170"
                     /inference="COORDINATES: protein motif:HMM:NF022321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2703 domain-containing protein"
                     /protein_id="HZH93671.1"
                     /translation="MKTLIIEWRHLDVKGETCDRCSQTGANLVDEINLLNRKLKSLEI
                     QVMLKENKLDEVRVNESNMILINNVPLENIIDIRVVDNFCKSCSDLVGSETYCRAVYY
                     KGKKFDDVPRIAIREAVAKSLGLRIRKKKG"
     gene            1036..1581
                     /locus_tag="VFD79_06175"
     CDS             1036..1581
                     /locus_tag="VFD79_06175"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HZH93672.1"
                     /translation="MRELWEIFVSFFKMSSVTFGGGYAMLPILQREIVDGKKWMTEER
                     IIDYYAVSQGLPGIIAVNVAIFIGKERKGIAGGIAGALGIVGPCLFIITFIAAFLSNF
                     QDNPLVRNAFAGIAVCVSALILNSVIGLWKKGVVDKTGIAVFAIVFIGMMATGLSPIV
                     LIVASAIFGIVSRKIAGGGIK"
     gene            1578..2147
                     /locus_tag="VFD79_06180"
     CDS             1578..2147
                     /locus_tag="VFD79_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HZH93673.1"
                     /translation="MIFAKLFYEFLKIGFFTIGGGMATIPFLQDLSESTGWFSIAFIA
                     DMVAISESTPGPIGINMATYVGYNVAGILGGIIATLGLVIPSIIIVSVVSGYLERFRK
                     SPLVEYVFYGLRPAVTGLIAAAGVEIVRIAVLDNELYKATNNLRDLLDWRKALYFVII
                     FYLIRRFKRHPVAYIGLSAIIGIAFGFAN"
     gene            2434..>3120
                     /locus_tag="VFD79_06185"
     CDS             2434..>3120
                     /locus_tag="VFD79_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93674.1"
                     /translation="MKKKLVIALVVFTAVAAGIFYLLTMGNIGTRYNTAQVEKSEVGR
                     FVQETGKVSSRNIRKYYGNSANKVESLNVQLGDHVASGQLLIEFEDNLDLEIQKVEKQ
                     IEALEATYSDATRGTDIESINSAKIEIARINSNIEMANKNKERIEELYNNGAVALVEL
                     EQAQSNLSQQQSNLEVAQNNYNQLMKGVSASTRKRYEAEIDVLLLTMESLKRSKEITS
                     IYSDIEGIVTE"
BASE COUNT          978 a          562 c          712 g          868 t
ORIGIN      
        1 ggtcgggaca agtctgacct atgaatcctg gtatttctgc gacaatgagg aggatgccaa
       61 tgagctggcc cagctggttc tggaaggaac aaaaagggca acggcatcac tttataagag
      121 ctatgagttt gagaaagaag agctgccaaa ggaaggaagc cacattgttg ttacggattg
      181 gaatggaact gctaaatgca taattaggac caacaaagta tccattttac ccttcaaaga
      241 tataacagaa gaccatgcta gaatagaggg tgaaggtgac aagtccctta aatactggaa
      301 agatgggcac acaaggtttt tcactaggga atgtgaagtc atgggaattg agttcaacca
      361 ggatatgctt gttgtatttg aagagtttca ggtggtttat ccataaaaca taagagggtc
      421 cagaattcat catgttgaat tctggaccta gatcttttca tccttttttc ttgcggattc
      481 tcaaacccaa tgatttggca acggcctccc tgattgcaat ccgaggcaca tcgtcaaatt
      541 ttttaccctt ataatagaca gccctgcagt aggtctcaga acccaccagg tctgaacatg
      601 atttgcagaa gttgtccact accctaatgt cgatgatatt ttcaagggga acattattta
      661 ttaggatcat atttgactca tttaccctta cctcatctag cttgttttcc ttaagcatca
      721 cctggatctc aagtgatttc aactttctgt tcaagaggtt tatttcatcc actaagttgg
      781 cacctgtttg gctacaccgg tcacaggttt ctcccttgac atccaggtgt ctccattcaa
      841 ttattaatgt tttcattgtt ggcctccaat atgtatatat tgaacttcta agaaaataat
      901 acacctgaat caggattttt acaacttatt ggaataatca atccaattaa gtccaatacc
      961 aatgcatatt ccttggaaat attgtacaat aaggtaagat gcaagaaatt gactaattga
     1021 caaaggatga tggacatgag ggaattgtgg gagatattcg tctcgttttt caagatgagc
     1081 tcagttacct ttgggggagg ctatgccatg ctgccaatcc tgcaaaggga aattgttgat
     1141 ggcaagaaat ggatgacaga ggaaagaata atagattact acgcagttag ccaggggctt
     1201 cctggcatca ttgctgtgaa tgttgctatt ttcataggta aggaaaggaa gggaatagct
     1261 ggaggaattg ccggtgccct tggaatagtc ggtccatgcc tgtttataat aacctttatt
     1321 gctgcatttc taagcaactt tcaagataat ccactggtaa ggaatgcttt tgcgggaata
     1381 gccgtgtgcg tttcagcgct tatattaaat tcagtaatcg gtctatggaa aaagggcgtt
     1441 gtggacaaga ctggaattgc agtctttgca atagtattca tcggtatgat ggctactgga
     1501 ctttcaccaa tagtattaat cgtagcttca gcaatttttg gaattgtttc cagaaagatt
     1561 gcaggaggtg ggattaaatg atatttgcaa aactattcta tgagttctta aagattggat
     1621 ttttcacaat cggaggtgga atggccacaa taccattcct tcaggacctg tccgaatcaa
     1681 ccggctggtt ctccatagca ttcattgcgg acatggtagc catatcagag tcaacaccgg
     1741 gtcccatagg gataaacatg gccacctatg taggttacaa tgtggcaggg atccttggag
     1801 gaatcatagc tacactggga cttgtaatac catcaataat tatcgtatca gttgtatcag
     1861 gctatctgga aagattcagg aagagccccc ttgtggaata tgtattttat ggcttgaggc
     1921 ctgctgtgac tggccttatt gcggcagccg gagttgaaat agtaagaata gccgtgcttg
     1981 ataatgagct ttacaaggct accaataacc ttagggacct tctggattgg aggaaggcgc
     2041 tatattttgt tatcatcttt tacctgatca gaagattcaa aaggcatccg gttgcataca
     2101 taggtttatc agcaattata ggaatagcat ttggatttgc caattaaatc gccaagggat
     2161 gaagccctcc atttgagggt tccatccctt gctttgtatc catgtaagca gctggaactt
     2221 atatttataa tcaaatgttc aaatttacat aaatttgaat ggtgtcaaat ggttttaaag
     2281 agaattagcc gggtatttat cttaatgaca cagtaaagtg aaattgattt gtcaaataac
     2341 aagataaaag gatcctacca aaaaataatc agggtgatat gacctggata aacactgatt
     2401 caagcatatg aaaacctaga aatggaggta gccatgaaaa agaaattagt cattgcattg
     2461 gttgttttta cggctgtagc agctgggata ttctatcttc tgaccatggg aaatatcggt
     2521 acaaggtaca atactgccca ggtagaaaag agtgaagtgg gtaggtttgt ccaggagact
     2581 gggaaagtca gctcgagaaa catcagaaag tattacggta acagtgcaaa caaggtagaa
     2641 tctttgaatg tccagttggg ggaccatgta gcaagtgggc agcttttgat agaatttgag
     2701 gacaatctgg acttggagat ccaaaaggta gaaaagcaga ttgaagccct ggaagctaca
     2761 tattccgatg caacaagagg cactgatatt gaaagcataa acagtgctaa aattgaaata
     2821 gccaggatta attccaatat tgaaatggca aacaagaaca aggagaggat tgaagagctt
     2881 tacaacaacg gagctgtagc tcttgtggaa cttgagcagg cacagagcaa tctttcccaa
     2941 cagcaaagca accttgaagt tgcccagaac aattacaatc agctgatgaa aggggtctca
     3001 gccagcacca gaaaaagata cgaggcagaa atagatgtcc ttctccttac catggaatca
     3061 ctgaaaagaa gcaaggaaat caccagcatc tattcagaca tagagggaat agtgacagag
//
LOCUS       DASRQF010000235         3390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_334310, whole genome shotgun sequence.
ACCESSION   DASRQF010000235 DASRQF010000000
VERSION     DASRQF010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334310"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1313
                     /locus_tag="VFD79_06190"
     CDS             <1..1313
                     /locus_tag="VFD79_06190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HZH93675.1"
                     /translation="KLVNNLSLSLVGRTAGGPAKVSVVVSGFLGMINGSAIANVATTG
                     IITIPMMKETGYKKEFAAGVEAAASTGGQFLPPVMGAVAFLMAEYLGESYAVVALAAA
                     VPAVLYYFGLLMSVHFEAKRLGLRGLDRENIPNALKVLKREGHLMAPLISLLGLMALG
                     YTPLYACVVSIFVVIGSSWVRKHTRMGIRTIIQACEDGVRGVISVGISCVIIGIIVGT
                     VSLTGLGLKFGYLMLQVVGPGELIKAGVMVAIMSIILGMGVPGIAAYVIVTAVAVPVM
                     VQVGALPIAAHLFCLIYASLSNITPPVAMSVYVASGIADSDFRKTGIQAVLVGLSGFI
                     LPFFFLVNPILLLGAAPEGTGILETAVAILGAIFGIYFLASGTGGWYIRRSGLVERLA
                     AILVGLLLIHPVGGTDAVGVGIAAFLTIYQLQKNKGDKAKKINA"
     gene            1344..2315
                     /locus_tag="VFD79_06195"
     CDS             1344..2315
                     /locus_tag="VFD79_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HZH93676.1"
                     /translation="MKRILSMALAFVLLAGVLAGCGNGGNEDVTHLKFPTASTTGAIY
                     PIGAAISNLWNENIEGLNVSVEASNGGVQNLGLVARGEAQIGVAVTNIMTEQKNGTGV
                     FEGKQYDGIRVLTALYSNYNQVVVGGDSGIESLSDIRGKVFAPGAPGSTPEVETNIHL
                     TAAGLNYPDDFSPQFVGFTESIDLIRNKQLDGAWLQAGIPTSAVSEILSTANGRLVSI
                     DEDVITQLTEEYPWYNRAVIPAGTYENQDYDVVTTSIVITIFVDESLPEETAYQMTKV
                     FWENVESLASTHNAIKGLELEQAVQNLAGLPLHEGSERYYREMGIMD"
     gene            complement(2357..>3390)
                     /locus_tag="VFD79_06200"
     CDS             complement(2357..>3390)
                     /locus_tag="VFD79_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93677.1"
                     /translation="KDWRQVMIDLTEANAGDEKALQEALDAEIDRYSKAFWNLPVDEQ
                     SIVASDLDENYSRMAYPTKADIEKLTQDYKKNLYDRLYPVATSVRNYFMKKAEAEYLK
                     ELNKAKSFYNQTIRLSIEEDLADGEQSQYAGYTMRLTPLKETAEKRTWTGTISDEGKV
                     AARMTLLGYILAGSPNTVALFKPGDDPDLDQPELEIPFTLSAPETLVKIGKGLPLEAV
                     LGNYAMTTTTSFGDSSFSEDMDIQILHSGGKISIINSYGTFFYWTDNTSEADNAEYVY
                     KFDYDPSISRLTVDILETRDNGNSINMVELWTFSEENGKIVFSGTVEIRKDGQSAMMP
                     QVRTGVKVD"
BASE COUNT          900 a          725 c          863 g          902 t
ORIGIN      
        1 gcaagctggt gaacaacctg tccctttccc tggtgggaag aaccgcaggt ggaccagcca
       61 aggtatccgt ggtggtttca ggatttttag gaatgataaa cggatctgct atagccaatg
      121 ttgcaacaac cggaataata accataccaa tgatgaagga aacaggctac aagaaggagt
      181 ttgcagcggg agtagaggcg gctgcatcca caggaggcca gtttctgccg cctgtaatgg
      241 gtgctgtggc cttcctgatg gctgaatacc ttggagagtc ctatgcagtt gtggcccttg
      301 ccgccgctgt gcctgcagtc ctctactatt ttggcctcct tatgtcagtc cactttgaag
      361 ccaagaggct ggggcttagg gggctggaca gggaaaatat tcccaatgcc ctaaaggtat
      421 taaagaggga aggccacctc atggcacccc tgatatccct tctgggactt atggccttgg
      481 gctacacacc cctttatgcc tgcgtggtat caatatttgt ggtgatagga tcaagctggg
      541 tcagaaagca taccagaatg gggattagga ctatcataca ggcctgcgag gatggggtaa
      601 ggggagttat cagcgtagga ataagctgtg tgataatagg aatcatcgtg gggacagtct
      661 ccctgacagg acttggactt aaattcggat accttatgct ccaggtggtt ggcccggggg
      721 aacttataaa ggctggagta atggttgcca tcatgagtat aatcctggga atgggagttc
      781 ctggcattgc ggcctatgta atagtcacgg cggttgcggt acctgtaatg gtccaggttg
      841 gagcccttcc aatagcagcc catcttttct gcctgatata tgcatccctt tccaacataa
      901 ccccgcctgt ggcaatgtct gtatatgttg cctcggggat tgcagattct gacttcagaa
      961 agacagggat ccaggctgtc cttgttggat tgtcgggatt tatcctgccc ttcttctttc
     1021 ttgtgaatcc aatactcctt cttggagcgg ctccagaggg tacgggaatt cttgaaactg
     1081 cagttgcaat attgggtgca atatttggaa tatacttcct ggcatccgga acaggagggt
     1141 ggtatattag aaggagtgga ctggtggaaa gactggcggc aatacttgtc gggcttctac
     1201 ttattcatcc cgtaggaggt acggatgcag tgggagtggg gattgcagca tttcttacaa
     1261 tatatcagct acaaaaaaac aagggtgaca aggcaaagaa aatcaatgcc tgaaacatat
     1321 aaagctttag agaggagaaa tgtatgaaaa gaatattatc tatggcactt gcttttgtgc
     1381 ttttggctgg agtccttgca ggctgtggga atgggggaaa tgaagatgtt acccatctta
     1441 agtttccaac ggcatccact accggggcta tctatcctat cggtgcggca atatcaaacc
     1501 tttggaatga gaacatagag ggactgaatg tatcagttga ggcatcaaat ggaggtgtcc
     1561 agaatcttgg cctggttgcc cggggagagg cccagatagg tgttgccgta acaaacatta
     1621 tgactgaaca aaaaaatggt acaggggtat ttgagggtaa acagtacgat ggaataaggg
     1681 ttcttacagc actatattca aactacaacc aggtggttgt tggaggggat tctggaatcg
     1741 aaagcctgag tgatataagg ggtaaggtat ttgctcctgg agcaccagga agcacacctg
     1801 aagttgagac aaacatccac cttacagctg caggcctaaa ctaccctgat gacttcagcc
     1861 cacagtttgt aggctttaca gagtcaattg acctaataag aaacaagcag cttgacggag
     1921 cctggctcca ggcaggaatt ccaacctcgg ctgtgtcaga aatcctgtca acggcaaatg
     1981 gaaggttggt ttcaatagat gaggatgtca taactcagct gacagaggag tacccctggt
     2041 acaatagggc agttatccct gctggaacct atgaaaacca ggattatgat gttgttacaa
     2101 ccagcattgt aataaccata tttgtggatg aaagcctgcc ggaggagacg gcttaccaga
     2161 tgacaaaggt cttctgggag aacgttgagt cccttgcaag tacccacaac gccataaagg
     2221 gtcttgagct ggaacaggca gtccagaacc tggcgggact tccgctccat gaaggttctg
     2281 aaagatacta cagggaaatg ggaataatgg attaaataga gcaagcacaa gggatattga
     2341 cccttgtgct tgtcttttaa tctaccttga cgccagttct cacttgaggc atcattgcag
     2401 attgaccatc ctttcttatc tcgacggtac ctgaaaatac aatcttgcca ttttcttctg
     2461 aaaaggtcca tagttcgacc atattaattg aattgccatt gtctcgggtt tcaagaatat
     2521 caaccgtaag ccttgaaatg gaagggtcat aatcaaactt ataaacatat tctgcattat
     2581 cggcttctga tgtattatct gtccagtaga agaaggtgcc ataagaattg ataatactga
     2641 tcttgccacc agaatggaga atctgtatgt ccatatcctc tgagaagctg ctatctccaa
     2701 agcttgtggt agttgtcatg gcgtagttgc caaggactgc ctccaaggga agtcccttgc
     2761 cgattttaac cagggtttca ggagctgaca gggtaaatgg aatctcaagc tcaggctggt
     2821 caaggtcagg atcatcacca ggcttgaaaa gagcaaccgt atttggagag ccagccaaaa
     2881 tataaccaag cagggtcatc ctggctgcaa cctttccttc atctgaaatt gtaccggtcc
     2941 aggttctctt ctcagcggtt tccttaagag gggtgagtct catggtgtaa cctgcatact
     3001 gtgactgttc accatctgca agatcttcct cgattgaaag tcttatggtt tggttgtaga
     3061 agcttttagc cttgttaagc tcctttaggt actcagcctc agcctttttc ataaagtagt
     3121 tcctaactga tgttgcaaca gggtagagcc tgtcgtaaag attcttttta tagtcctggg
     3181 ttagcttttc aatatcagcc ttagtcgggt atgccatcct ggagtaattc tcatcaagat
     3241 ctgaagccac aatggactgt tcatccaccg gaaggttcca gaaagccttt gagtacctgt
     3301 cgatttcagc atcgagtgcc tcctgtaatg ccttctcatc tccggcattg gcctctgtaa
     3361 gatcaatcat aacctgtctc cagtccttaa
//
LOCUS       DASRQF010000236         6537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1272924, whole genome shotgun sequence.
ACCESSION   DASRQF010000236 DASRQF010000000
VERSION     DASRQF010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6537
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272924"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..600)
                     /gene="recR"
                     /locus_tag="VFD79_06205"
     CDS             complement(1..600)
                     /gene="recR"
                     /locus_tag="VFD79_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HZH93678.1"
                     /translation="MDYYALPIANLVDQFSKLPGIGRKTAQRLAFHVLEMDQQDAEKL
                     AAAILEAKEKIGFCQVCGNLTDKPTCNICSSHNRDKSVICAVEGPRDIVAMERSKEYK
                     GLYHVLHGTISPMENIGPQDIKIKELLHRLEDDEVKEVVIATNPTVEGEATAMYISKL
                     IKPLGVKVTRIAHGIPVGGDLEYFDEVTLARAMDNRREM"
     gene            complement(621..956)
                     /locus_tag="VFD79_06210"
     CDS             complement(621..956)
                     /locus_tag="VFD79_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HZH93679.1"
                     /translation="MARGGYPGMGGNMNNMMKQMQKMQKKMEEVQAQVDATELEATSG
                     GGAVKVVVNGKREVLDIEIDPSVVDPEDVEMLQDLVMAAVNEALRKAEEFASKEMGKV
                     TGGLNIPGM"
     gene            complement(970..2637)
                     /gene="dnaX"
                     /locus_tag="VFD79_06215"
     CDS             complement(970..2637)
                     /gene="dnaX"
                     /locus_tag="VFD79_06215"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HZH93680.1"
                     /translation="MYQALYRQYRPKTFDEILGQTHITTILKNQIKKDNIGHAYLFSG
                     TRGTGKTSAAKVFARAVNCLQPQNGNPCNECAICKGILDESLMDVIEMDAASNRKIED
                     IRELKEKVIYPPSKARFKVYIIDEVHMLTNEAFNALLKTLEEPPKHLMFLLATTEPER
                     LPQTILSRCQRFDFKRLSANDMVGGMKKITDKLGIDVEDKVLQLIAKNSDGAMRDALS
                     LLDQCIAFEGDSVSYDDAINILGIANVDLIFDLVNAIGDESTEKSLLMVDHMIQEGKD
                     IPQFLKDMIHHYRNLVIAKTSKNPEALIEWGEVEEYKEQSSKMDLHYLMSSLQALTSA
                     ETSMKWSMQPRIILEMAVIKLANLKNELSLEERVRRLEEGIATAPTNETKVTYKSAPK
                     QEPIKPRAKASENLVKKEKSIPEEKEKPGVEKKEATGPSGELTLDNIRSSWPKVMQLI
                     KSRKINIYALVMEGEVSQLQGNRITLSYKDGFEFHKDAVNSNQNKTFLEEILTEFFGQ
                     KIELLVIMKGCEMPAAGGGKQEDEKSAREETVNKVLDFFGEDIVEIK"
     gene            complement(2738..2977)
                     /locus_tag="VFD79_06220"
     CDS             complement(2738..2977)
                     /locus_tag="VFD79_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255349563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(2939..2941),aa:Sec)
                     /transl_table=11
                     /product="Rdx family protein"
                     /protein_id="HZH93681.1"
                     /translation="MKNKISIEYCTSUGYLSRAVALSESILNKHKNQVSELTIIPSGG
                     GVFEIKVNDKLLFSKAELGRFPEEGEAEDLVKEAI"
     gene            3096..3187
                     /locus_tag="VFD79_06225"
     tRNA            3096..3187
                     /locus_tag="VFD79_06225"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3132..3134,aa:Ser,seq:gga)
     gene            3201..3467
                     /gene="ffs"
                     /locus_tag="VFD79_06230"
     ncRNA           3201..3467
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFD79_06230"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            3611..4201
                     /locus_tag="VFD79_06235"
     CDS             3611..4201
                     /locus_tag="VFD79_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93682.1"
                     /translation="MSLNLCIDIDGTITEAYYWLKAANRYFDADIQPYQVKEYDIHNV
                     LNVPREAYQEFYRLHWRELHGEARPRKYADLVLWKLFMQHNISYVTAREPATRDVTVQ
                     WFRKHGFPEMDLHMLGSHYKVEKAKELDCDVFVEDRYENAVELALSGVQVLLMDCSYN
                     RKPLIPGITRVTNWLDVHEEIETIKSKKQKLAPQIA"
     gene            complement(4294..>6537)
                     /gene="clpB"
                     /locus_tag="VFD79_06240"
     CDS             complement(4294..>6537)
                     /gene="clpB"
                     /locus_tag="VFD79_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002835779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HZH93683.1"
                     /translation="LAILSSKGTHSGDIFREYGITRDLFLQSLMKVRGSQTITSDNPE
                     GTYEALERFGRDLVEEARKGKMDPVIGRDSEVRNVIRILSRRTKNNPVLIGDPGVGKT
                     AIVEGLAQRIVNGDVPEGLKDKTIFALDMGALVAGAKYRGEFEERLKAVLTEIQKSDG
                     RILLFIDELHNIVGAGRAEGSMDAGNLLKPMLARGELHTIGATTLDEYRKYIEKDAAL
                     ERRFQKVLVTEPTVEDTISILRGLKEKYEIHHGIRISDGAVIAAATLSDRYISDRFLP
                     DKAIDLMDEASAMIRTEIDSMPVEIDEVRRRILQLEIEREALKKETDDASRTRLEKLE
                     RELGELKSEFDLLKAQWEAEKKEISAEKAIKEDIDRVKREIEEAERSYDLEKLSQLKY
                     GSLVELEGKLKEAQGKEHTEERLLKEEVTEEEIAEVVAKWTGIPTTKLLATEREKLLG
                     LGEILHRRVIGQDEAVDSVVDAVLRARAGLKDINRPVGSFIFLGPTGVGKTELSKALT
                     ETLFDDEKNIIRIDMSEYMEKHSVSRLVGAPPGYVGYDEGGQLTEAVRRKPYSVILFD
                     EIEKAHPDLFNILLQVLDDGRLTDNQGRTVDFKNTVIIMTSNIGSEYLLEGLGEDGTI
                     QESTRDRVMSQMRMTFRPEFLNRIDEIVLFKPLQRMEIFKIIDLQIAEIEKRLQDRSI
                     TIKVTEEAKENILERSYSIQYGARPVKRFLQKEIETGISRMIIRGELKDGDTVKIHAV
                     EGELLIG"
BASE COUNT         1514 a         1702 c         1296 g         2025 t
ORIGIN      
        1 ctacatctct cttcgattgt ccattgcacg tgccagggtt acctcatcaa agtattccag
       61 atctccgccc accggaattc catgggctat ccttgtaacc ttgactccca agggcttgat
      121 aagcttggag atgtacatgg cggttgcctc tccctccaca gttgggttgg ttgctattac
      181 aacctccttg acctcatcat cctccagcct gtgcaaaagc tcctttatct tgatgtcctg
      241 tggtccgata ttctccatgg gtgatatggt tccatggaga acgtggtaca agcccttgta
      301 ctccttgctc cgctccatgg ccacaatatc ccttggtccc tccacggcac atatgacaga
      361 cttgtccctg ttgtggctgc tgcagatgtt acaggttggc ttgtccgtaa gattcccaca
      421 tacctggcag aaaccaatct tctccttggc ctcaagtatt gcagctgcaa gcttctcggc
      481 atcctgctgg tccatttcca gtacgtggaa tgccaatctc tgggcggtct ttcttccaat
      541 tcccggtagc tttgagaact ggtccactaa atttgcgatt ggcaatgcat aataatccat
      601 ctcttatcct ccagttattc ctacattcca ggaatattta gtcctcctgt caccttgccc
      661 atttcctttg atgcaaattc ttcagctttt ctcaatgcct cgttaacagc tgccataaca
      721 agatcctgaa gcatttctac atcctccgga tcaacaactg aaggatcaat ctctatgtct
      781 agaacctctc tcttgccatt tacaacaacc ttcactgctc ctccccctga agttgcctca
      841 agctctgtag catcaacctg ggcctgaacc tcctccatct tcttctgcat cttctgcatc
      901 tgcttcatca tgttgttcat atttccaccc attcctgggt atcctcctct tgccatcatt
      961 atctcctcct tatttaatct ccactatatc ctcaccaaag aaatccagta ccttgttgac
     1021 tgtctcctcc ctggcggatt tttcatcctc ctgctttccg cctccggcag caggcatttc
     1081 acaccctttc attataacaa gaagttctat tttttgtcca aaaaattccg taagaatttc
     1141 ctctaaaaat gtcttgttct gattggagtt tacagcatcc ttgtggaact cgaagccatc
     1201 cttgtaggaa agggttattc gatttccctg aagctgtgaa acctctccct ccataaccag
     1261 ggcatatatg tttatttttc ttgattttat caactgcatg acctttggcc aggagcttct
     1321 tatgttatca agggtcagtt ctccacttgg gcctgtagcc tctttcttct ctactccagg
     1381 cttttccttt tcctctggga tgctcttttc tttcttcaca agattctcgg aagctttcgc
     1441 ccgaggcttt ataggttcct gtttgggagc tgatttgtat gtgactttgg tttcatttgt
     1501 tggagcggtt gcaattcctt cctcaagcct tcttaccctt tcctcaaggg acagctcatt
     1561 cttcagattg gcaagtttta taaccgccat ctcaagtatg attcttggct gcattgacca
     1621 cttcatggaa gtctcagctg atgtcaatgc ctgaaggctg gacatgagat aatgaaggtc
     1681 catttttgaa gactgttcct tgtactcctc aacctctccc cattcaataa gggcttcagg
     1741 atttttcgag gtcttggcaa taaccaggtt tctgtagtga tggatcatat ccttcagaaa
     1801 ttgggggata tccttgccct cctggatcat atggtccacc ataaggaggg acttttcagt
     1861 gctttcatct ccaatggcat tcaccagatc aaaaatcaga tccacattgg cgatgcccaa
     1921 aatatttatt gcatcatcat aggatactga atcgccttcg aaagcaatac attgatccaa
     1981 tagactcagt gcgtccctca tggcaccgtc ggaatttttg gcaataagct gaagaacctt
     2041 gtcctcaaca tctattccaa gcttatccgt tatttttttc ataccaccta ccatgtcatt
     2101 tgctgaaagt ctcttgaaat caaaccgctg acatctggat agtattgtct gtggtaatct
     2161 ctccggttca gttgtagcca aaaggaacat aagatgcttt ggtggctcct ccagggtctt
     2221 taagagggca ttgaaggcct cattggtgag catgtgaacc tcatctatta tgtagacttt
     2281 gaaccttgcc tttgaaggag gatagatcac cttttccttc agctcccgaa tgtcctcgat
     2341 tttcctgtta ctggctgcat ccatctcgat tacatccatt aggctttcat caagaatccc
     2401 cttacatatt gcgcattcat tacagggatt gccattctgg ggttgtagac aatttacagc
     2461 ccttgcgaaa accttggctg cagaggtctt tccagttccc ctggttccag aaaaaagata
     2521 ggcatgtcct atgttatcct ttttaatctg gttctttaaa attgtggtta tatgtgtctg
     2581 gcccaatatc tcatcaaatg tcttaggcct gtattgtcta tataatgcct gatacatgga
     2641 atcaccctta cttgttttga aatcttacta cctcccatta tatcaaaaaa gctccctgtt
     2701 tacactagga aaagcaaagg gcccacttgg gcccctatta aatagcttcc tttacaagat
     2761 cctcagcttc gccctcttca gggaacctgc caagctctgc cttggaaaac aaaagcttgt
     2821 catttacctt gatttcaaat actccaccac cggatggaat tattgtgagc tctgaaacct
     2881 ggttcttgtg tttattcagg atactttccg acagagcaac tgccctgcta agataccctc
     2941 aggaagtaca gtattcaatt gatattttat tcttcatggc atttcctcct tcaattttat
     3001 ttccttatcc atttacccgg atttggatta tctaaacctg aacctggttg acattcctta
     3061 atttatggtc tataatataa gtctgtggaa accaaggaga gatggctgag tctggtcgaa
     3121 ggcgcacgac tggaaatcgt gtaggcgttt actcgtctcg agggttcgaa tccctctctc
     3181 tccgccatta gaacattttg ccgtgctaga tggggaggta gcggtgccct gttacctgca
     3241 atccgctaca gcaggattga attcccggct taggcttctc tgctgtgagg tctgcctcga
     3301 aaaagtggcg ttgacggttg ggtcccacgc aacggaaact catgaacctc gtcaggtccg
     3361 gaaggaagca gcgataagtg aaacttttcg tgtgccgtgg gtcagcctgg cccgagttaa
     3421 ctgttcgggt aacgctcgga gcaggattga cgaagctggg tgcacggcta cataatgaaa
     3481 acaactctgg aaaattccag ggttgttttt ttgtttaagg taaaagttta ccaaagcatt
     3541 acattatctt cattgaggct ttatattaaa ggtgtatgat gtaatcatag taagagattg
     3601 gagtgatcag atgagtttaa atctatgtat cgatatcgac ggaacaatta ctgaagcata
     3661 ctactggctc aaggcagcca acagatactt tgatgccgac atacagcctt accaggttaa
     3721 ggaatatgat atccataatg ttttgaatgt gccaagggag gcttaccagg aattttacag
     3781 acttcactgg agggagctcc acggggaggc aaggcccaga aagtatgcag accttgtact
     3841 gtggaagctt ttcatgcagc ataacatatc atatgtgacc gctagagagc cagcaacaag
     3901 agacgtcaca gtccaatggt tcagaaaaca tggtttcccg gaaatggatc ttcatatgct
     3961 gggttcccat tacaaggtgg aaaaggccaa ggagcttgat tgtgatgtat tcgttgaaga
     4021 caggtacgaa aatgcagttg agttggcact ttcgggcgtc caggttcttc ttatggactg
     4081 cagctacaac agaaaaccac ttattccagg aattacaaga gttacaaact ggctggatgt
     4141 ccacgaggaa attgaaacaa tcaagtctaa aaagcaaaaa ctagctcccc aaattgcatg
     4201 atttaatata gatcaaacaa cacaaaacag aaggtggatt ttccaaaaaa ggaataatcc
     4261 gccttctgtt tttccatagc atttttgctg tagttagcca attaacaatt ctccctctac
     4321 agcatgaatc ttaactgtgt ctccatcctt cagttcccct cttatgatca ttcttgaaat
     4381 acctgtttcg atctcctttt gcaggaacct tttcaccggt cttgcaccgt actggattga
     4441 ataggacctt tccaagatgt tttcctttgc ctcttcggtt accttgatgg tgatgcttct
     4501 gtcctgaagc ctcttttcaa tctcggcaat ctgaaggtcg atgatcttga atatctccat
     4561 cctctgcagg ggtttgaaca ggacaatctc atcaatacga ttcagaaatt caggcctgaa
     4621 ggtcatcctc atctggctca tgaccctgtc ccttgtggat tcctggattg ttccatcctc
     4681 accgaggcct tccagaagat actcggaccc gatgtttgac gtcatgatta ttacagtgtt
     4741 cttgaagtcc actgtccttc cctggttgtc tgtaagcctt ccatcgtcaa ggacctgaag
     4801 gaggatgttg aacaggtcag ggtgggcctt ctctatctca tcaaagagga ttacagaata
     4861 gggttttctt ctgacggctt ctgtcagctg gcctccttca tcatagccca catatcctgg
     4921 aggcgcccca actagccttg aaacggagtg tttctccata tactcgctca tatctatcct
     4981 gatgatgttt ttctcatcat cgaaaagggt ctctgtcaat gcctttgata gctcagtctt
     5041 tccaacaccg gtgggcccca ggaatatgaa gcttccaaca ggtctgttta tatccttaag
     5101 acctgccctg gcacgaagga cagcatccac aactgaatcc accgcctcgt cctggccgat
     5161 taccctcctg tgaaggatct cacccaggcc aaggagcttc tccctttctg ttgccagaag
     5221 ctttgtggtt ggaattccgg tccactttgc aacaacctcg gcgatctcct cttcagtgac
     5281 ctcctccttg agaagtcttt cctcagtgtg ctccttgccc tgggcctcct tcagctttcc
     5341 ttccagctct acaaggcttc cgtacttcaa ctgggacagc ttctccagat catagcttct
     5401 ctccgcctct tcaatttccc gttttaccct gtctatgtcc tccttgatcg ccttttcagc
     5461 tgatatttcc ttcttttcag cttcccactg ggccttgaga agatcaaact cactctttag
     5521 ctctccaagc tccctttcaa gcttttcaag tctggttctg gatgcatcat cggtttcctt
     5581 cttaagggct tccctttcga tctccagctg caatatcctt cttcttactt catctatttc
     5641 aacaggcatg gagtcgattt ccgtccttat cattgcagag gcctcgtcca tcaggtcaat
     5701 agccttgtca ggcaggaacc tgtctgagat atacctgtct gaaagggtgg ccgcagctat
     5761 taccgcaccg tctgatatcc tgattccatg gtggatctca tacttctcct ttagccccct
     5821 cagtattgaa attgtatcct ccactgtggg ctctgtcaca aggaccttct ggaatctcct
     5881 ttccagggca gcgtcctttt caatgtattt cctgtattca tcaagggtgg ttgctccgat
     5941 tgtgtggagt tctccccgtg caagcattgg ctttaagagg tttcccgcat ccatggaacc
     6001 ctccgccctg ccggcaccca caatattgtg gagctcgtca ataaagagca ggatcctgcc
     6061 gtcagacttc tgaatctctg taagcacagc ctttagtctt tcctcaaact cgcccctgta
     6121 ctttgctcct gccaccaggg ctcccatgtc cagtgcaaat attgttttgt ccttaagccc
     6181 ttcaggtacg tccccgttta ctatcctctg ggcaagtccc tctactattg cagtttttcc
     6241 aactccaggg tcacctataa ggaccgggtt gttctttgtt cttcttgaaa ggatcctgat
     6301 tacattcctg acctccgagt cccttccaat cacagggtcc atctttccct ttctggcttc
     6361 ctcaaccaga tcccgaccaa acctttcaag ggcttcatag gtcccctcag gattgtcgga
     6421 ggttattgtc tgacttcccc tgaccttcat tagagactga aggaataggt cccttgttat
     6481 tccatactcc ctgaagatat cacctgaatg ggtccccttt gaagagagta tagccag
//
LOCUS       DASRQF010000237         6533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1023478, whole genome shotgun sequence.
ACCESSION   DASRQF010000237 DASRQF010000000
VERSION     DASRQF010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6533
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023478"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VFD79_06245"
     CDS             complement(<1..823)
                     /locus_tag="VFD79_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZH93684.1"
                     /translation="MKTPVFEALKNLIEENSTHFHMPGHKGKNSLIEWGKYVPYIDTT
                     ETEGMDYLLEPRGIIKESQENAAKVFGSKATYYGVNGSTGSIYIALATITKPGDKVLI
                     QRNCHKAVYNALILNRLQPVYIYPNYNENHNVLTGLYPEDVDQALTENPEIKAVVMTY
                     PNYYGVCSDVKRIAEIVHKHNKILMVDEAHGPHMTFSDNLPISAIKGGADIVIHSTHK
                     TLPSLTQTSLIHVCSDRVDLNKLRDRYQLYTTTSPSYLFTLSNEIATAYMDGEGRE"
     gene            complement(948..1922)
                     /gene="amrS"
                     /locus_tag="VFD79_06250"
     CDS             complement(948..1922)
                     /gene="amrS"
                     /locus_tag="VFD79_06250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HZH93685.1"
                     /translation="MKEALFYKNHDGQVYCTLCPHYCKLSEGETGRCMARKNIGGKLY
                     SINYGKVTSYGFDPIEKKPLFHFHPGSTVFSFGSFGCNFRCDFCQNWQIVEDLSLYVE
                     MENSQILELSSLNGSIGVAYTYNEPSIFYEMVLDIAKEVHKTGGKNIMVTNGFLNREP
                     LENLLPHIDAMNIDLKSMSRTYYTTLCKGSLDPVLDTIKLAAGKTHVEITNLVIDGEN
                     SSLEEMEDLASFIGEIDKNIPLHLSRYFPHNRMENPPTEIETLEKLRKVAQRHLNHVY
                     IGNVWGINNDTLCSNCKSILIERFPETRVVGIDKGRCTNCGEKAPVVL"
     gene            complement(1935..3341)
                     /gene="amrA"
                     /locus_tag="VFD79_06255"
     CDS             complement(1935..3341)
                     /gene="amrA"
                     /locus_tag="VFD79_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HZH93686.1"
                     /translation="MGRLLSAYLTPHPPVLIHEIGQGQEKLAGDTLAAMEEIGRDIGK
                     RRPETIIVITPHGPLFSDAHSIALEESLKGDFGTFGHPELSYSFRNNLDLTYGIVKEA
                     LLEGVSLAQMTREMNKRYGLDGKLDHGVLVPLHFVDKYHSDFKLIPITYGLLSPGELH
                     IFGRAITRAIQAGKEDVCVIASGDLSHKLKSSGPYTYAREGPEFDKRIMDAIENGDLV
                     EIGGFPADLADKAGECGLKSLQILAGILSVYRISTRVLSYEGPFGVGYGTAVFDILGN
                     SDKDILHEISKRERRALEKVREREDPLVGLARKSLEHYVRNGDYLRLPEGLPDDLLNE
                     RFPVFVSIKKHGSLRGCIGATEPTMDNLAGEIIQYSVQAGTRDPRFPSVEEDELDQLT
                     YSVDVLMKPEPVEDKGGLDPSTFGVIVEKGHRRGLLLPNLDGVDTVEDQLRIALNKAG
                     IGPEEDFSIQRFKVVRHQ"
     gene            complement(3408..4049)
                     /locus_tag="VFD79_06260"
     CDS             complement(3408..4049)
                     /locus_tag="VFD79_06260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HZH93687.1"
                     /translation="MSDKKLLAAPRVGIGSGSAKKMRREKLVPGIVYSKGEETKHVQV
                     DQIDFVKVFRIVGTSSLMELEVDGEVIPVIVKSVQRNPVKGEVIHIDFQKLDMKQKIK
                     ITVPVYLLHRDAIKLQPSVLMQMLDTIEIECLPGDIPESVGIDVSGMDFETPLYVKDL
                     EIMGEDNVEVLTDPESIICTLNEPTMEEEEDGEAVEDPMSVPVVGEEEEGQDE"
     gene            complement(4129..4257)
                     /locus_tag="VFD79_06265"
     CDS             complement(4129..4257)
                     /locus_tag="VFD79_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93688.1"
                     /translation="MYEDNGVKVYLMKGMRIPDNKVEINLTNYLIAKSLTAKGIRY"
     gene            complement(4420..5583)
                     /locus_tag="VFD79_06270"
     CDS             complement(4420..5583)
                     /locus_tag="VFD79_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HZH93689.1"
                     /translation="MKRVILLLLLVTAFITAAMPTAFAMEAGMNEFETEMEFANYTER
                     GTVLQASEIEERPEDYFSVQNVTLRIDSGKFKGDIHEIENVLSDHAYYDIIVEEGQRV
                     SLMIEEYPDGGYNVYISDYYRINSIIYLLGAFVVLVVLIGGSKGVKSLISLGVTISAV
                     LFVMLPLILKGINPILVSVITAVLVTIVTIVLVAGFNHKSLSAIAGTSIGVIVAGVIS
                     YIVGNQASLSGLSAEEATMLMYIPQGIQFNFRHLLFAGIIMGALGAAMDVGISIASAI
                     DEIHKANPRLSARRLFRSGMNVGKDVMGTMVNTLILAYTGTSIPMLLLFMAYETDLSK
                     VFNLDVIATEIVRSLSGSIGLILTIPITAMIASYYAKDKRVAGDKDQEENEVI"
     gene            complement(5636..6313)
                     /locus_tag="VFD79_06275"
     CDS             complement(5636..6313)
                     /locus_tag="VFD79_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93690.1"
                     /translation="MQKILEPLGFLVYIAILIYMGIYIYRNHNGNKVHMAFGSLAVTL
                     AIGESTYFLPRIYALITTGLEDNINLIGWGRLGHLIVMTLFFSMLIDLNKECFGLRKK
                     VPLDKLLYGLLVFRVVIGIFPQNGHFQLQPDRTFLFLRTVPLVAYLVVVAIVIIICSW
                     KKGSNGMPLLAIMLLPLGLVIEPELFESSSYLVLVAKSVLRALLLLGIVFIGFKQVRK
                     ANELSRY"
     gene            6457..6532
                     /locus_tag="VFD79_06280"
     tRNA            6457..6532
                     /locus_tag="VFD79_06280"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6490..6492,aa:Val,seq:gac)
BASE COUNT         1593 a         1595 c         1375 g         1970 t
ORIGIN      
        1 tttcccttcc ctcaccatcc atatatgctg ttgcaatctc attggatagg gtgaagaggt
       61 atgacggact ggtggttgtg taaagctggt acctgtccct aagcttgtta aggtccaccc
      121 tgtctgagca gacatgtatc agggatgtct gagtcaggct tggcagggtc ttgtgggtgg
      181 agtgtattac aatgtcggct ccccccttga ttgctgaaat tggaaggttg tcggaaaatg
      241 tcatgtgtgg tccgtgtgcc tcgtcaacca tcaggatctt gttgtgcttg tggacgatct
      301 cagcgattct cttaacatct gaacaaaccc cgtagtagtt tggatatgtc ataactacag
      361 cctttatttc aggattttca gtcagggcct ggtccacatc ctcagggtat agccctgtta
      421 ggacattgtg gttttcattg tagtttggat agatgtagac aggctgtagc ctgttcagga
      481 tcaaggcatt atagactgcc ttgtggcagt ttctttggat taggaccttg tcacctggct
      541 tggttatggt tgccagtgcg atgtatatgc tgcctgtgga tccatttact ccatagtatg
      601 ttgctttgga accaaaaacc ttggcggcat tctcctggga ctcctttatt atcccccttg
      661 gctcaaggag gtaatccata ccttcagtct cagtggtgtc tatataagga acgtatttac
      721 cccattctat cagggaattc tttcccttgt gccctggcat gtggaagtgg gtactatttt
      781 cctcaattaa attcttaagt gcttcaaata caggtgtttt cattgtatat tgatcaacct
      841 cccaaatttt ttcttgaata acgtaataat tatagcgtag ctcacaatat aagtaaacta
      901 ttattttact atttgtaaac ttttttgcgg tttttttctt cctgtttcta aagcactact
      961 ggagccttct caccgcagtt tgtgcagcga cccttatcta taccaaccac cctggtttcg
     1021 ggaaatcttt caattaggat actcttgcaa ttggaacaaa gggtgtcgtt gtttattccc
     1081 catacatttc cgatgtatac atgattaagg tgcctctggg caaccttcct aagcttttcc
     1141 agggtctcaa tttccgttgg gggattctcc atcctgttgt ggggaaaata cctgcttagg
     1201 tgcaggggga tatttttgtc aatttcaccg atgaaagaag caaggtcttc catctcctcc
     1261 agggagctgt tctccccatc gattacaagg ttggttatct ccacatgggt ctttcctgca
     1321 gccagcttga tggtatcaag gaccggatcc aggctcccct tgcaaagggt ggtgtagtag
     1381 gtcctggaca tggatttcag gtcgatgttc atggcatcaa tatgggggag gaggttttcc
     1441 agcggctccc tgttgagaaa gccgtttgtc accatgatgt tcttacctcc ggttttgtgg
     1501 acctcctttg caatgtccaa aaccatttca tagaagattg agggttcatt gtaggtataa
     1561 gctactccaa tggacccgtt aagacttgaa agctccagaa tctgggaatt ctccatctcc
     1621 acatagaggc ttaagtcctc tacaatctgc cagttctggc agaaatcaca cctgaagttg
     1681 cacccaaagc taccaaagga aaagactgtg gacccggggt ggaagtgaaa caggggtttc
     1741 ttttctattg gatcaaagcc ataggaggtt accttcccgt agtttatgga atacaacttg
     1801 ccccctatgt tttttcttgc catgcacctg ccagtttcac cctctgacag cttgcagtaa
     1861 tggggacaca gggtacagta gacctgtccg tcatggtttt tgtagaacaa agcttccttc
     1921 atggtaatca gctcctattg gtggcgcacc accttaaacc tttggattga aaaatcttcc
     1981 tctggaccta ttccggcctt gttaagtgca attctcagct ggtcttccac cgtgtccaca
     2041 ccatcaagat ttggcagcaa aagacctctt ctgtgccctt tctctactat taccccaaat
     2101 gtggatgggt ctaaaccgcc cttatcctca acgggctcgg gcttcataag gacatcaaca
     2161 gaataagtca gctggtccaa ctcgtcctcc tccacagatg gaaatctcgg atccctggtg
     2221 ccagcctgga cgctgtattg gatgatttcc ccagccagat tgtccattgt aggctcggtt
     2281 gcccctatgc acccccttag ggacccgtgc ttctttatgg atacaaagac aggaaacctt
     2341 tcattcagaa ggtcgtcggg aaggccctct ggaagcctca ggtagtctcc atttctcaca
     2401 taatgctcaa gactttttct ggcaaggcca acaagggggt cctccctttc tcgcactttt
     2461 tccaaggccc tcctctccct ctttgaaatc tcatgaagga tgtccttgtc tgaatttccc
     2521 aatatatcaa atacagctgt tccgtatcca acaccaaagg gcccttcata ggacaggact
     2581 cttgtggata tcctgtagac agatagtatg ccagcaagaa tctgaagtga tttcaaacca
     2641 cattctccag ccttgtctgc aaggtctgca ggaaagcctc caatttcgac caggtcacca
     2701 ttttctatgg catccatgat cctcttgtca aattcaggtc cttcccttgc ataggtatag
     2761 gggccggaac ttttaagctt gtgggagagg tcgccgctcg ctattacaca tacgtcctct
     2821 tttccagcct ggattgccct tgtgattgcc cttccgaata tatgaagttc tccgggggaa
     2881 agaagtccgt aggttatggg gataagcttg aagtcagaat ggtacttatc cacaaagtgt
     2941 agtggcacca ggactccatg gtcaagtttt ccatcaaggc cgtacctctt gttcatttcc
     3001 cttgtcatct gtgccaggga aaccccctcc aacagtgcct ccttgactat cccgtaggtc
     3061 aggtcaaggt tgttcctgaa ggaatagctg agctcagggt ggccaaaggt tccaaagtct
     3121 cccttgaggc tctcctcaag ggctattgaa tgggcatcgg aaaacaaggg accatgtggt
     3181 gttatgacga ttatggtttc tggcctcctc tttccaatat cccttccaat ttcctccatt
     3241 gcagccaggg tgtctccagc aagcttttcc tgaccctggc cgatctcatg gattaggact
     3301 ggagggtgtg gtgtcaggta tgccgataat agtctcccca tttctgcttc ctcctttcat
     3361 aggaaaaaaa aagacagaca ccatggtctg tccctgtttc caagttatta ttcgtcttgt
     3421 ccttcctctt cttcaccaac aactggtaca gacattggat cctcaaccgc ttccccgtct
     3481 tcctcttcct ccattgtagg ctcgttgagg gtacagatga ttgactcagg atctgtaagg
     3541 acttcaacat tgtcctcacc catgatctcc aggtccttaa cgtaaagtgg agtctcaaaa
     3601 tccattcctg atacatctat gcctactgac tcaggaatat ctcctggcag acactcgatc
     3661 tcaattgtat caagcatttg cataagtacc gatggctgaa gctttattgc atccctgtgt
     3721 agaagatata ctggtactgt gatctttatc ttctgcttca tgtccagttt ctggaagtct
     3781 atgtgaataa cctctccctt gacaggattt ctctgaaccg acttaactat tacaggtata
     3841 acctctccgt ccacttcaag ctccattagg gatgaagttc caacgatcct gaataccttt
     3901 acaaagtcaa tctggtcaac ctgcacgtgc tttgtctctt cacccttgct gtacacgatt
     3961 cccggcacca gtttttccct tctcattttt ttcgcactac ctgacccaat ccccactctt
     4021 ggagcggcca gtaatttttt atcactcata ctataatacc tcctggtttt cttatttgat
     4081 ataagtcata tctaccctat taaaaaggct gataatcata tttctaccct agtagcggat
     4141 gcctttggcc gttaggcttt tggctattaa atagtttgtc aggtttattt ccaccttgtt
     4201 gtctgggatt ctcatcccct tcatgagata aaccttaacc ccgttgtcct catacatgtc
     4261 aaacctcttg ccatcacctt gatttcctat cctgaccgat agttgattgg tctggcatca
     4321 gccggatgca acggttacaa tggccacggt aacctcaggc tccccttcgg atttgttcct
     4381 gatgtagtct cttgccttgt ccgttactgt cactttcatc tatatcacct cattttcctc
     4441 ctggtctttg tctcctgcca ccctcttatc ctttgcataa tagcttgcta tcattgcggt
     4501 tatgggtatg gtcaatatca gtccgatgct tccggaaagg gaccttacaa tctcagttgc
     4561 gattacatcc agattgaaga ccttgctaag gtctgtctca tatgccatga aaagcagaag
     4621 cattggtatg gatgttcccg tgtatgcaag aataagggtg ttgaccattg tacccatgac
     4681 atcctttcca acattcattc cagaacggaa caaccttctt gcggaaagtc ttgggttggc
     4741 cttgtgtatt tcatctattg cagaggctat tgagattcca acgtccatgg ctgcaccaag
     4801 ggcacccatg attatccctg caaatagtag atgcctaaaa ttaaactgta ttccctgtgg
     4861 gatatacata agcattgtgg cttcctcggc ggaaagccct gaaaggctgg cctggtttcc
     4921 tacaatgtag gatatcacac ctgcaacaat tacgccaatg gaagttccag cgattgcgga
     4981 aagggacttg tggttgaaac ccgccaccag gacgattgtc actatagtta ccagaacagc
     5041 agttattaca cttacaagta ttggattaat ccccttaagt atcaggggca gcattacaaa
     5101 gaggaccgct gatattgtaa ctccaagtga aatcagggat tttaccccct tggaccctcc
     5161 aatcaggaca acaagaacta caaatgctcc caaaaggtag ataatgctgt ttatacggta
     5221 gtagtcagaa atatatacat tgtagcctcc atccgggtat tcctcaatca taagtgaaac
     5281 cctctgaccc tcctccacta ttatatcata ataggcatga tcagacagaa cgttttcgat
     5341 ttcgtggata tctcccttga attttccgga gtcaatcctt aatgtaacat tctggacact
     5401 gaaatagtcc tctggccttt cctctatttc tgaagcctga agcacggttc ccctttcagt
     5461 gtagtttgca aattccattt cggtctcaaa ttcattcatc cccgcctcca tggcgaaggc
     5521 cgtgggcatg gcagctgtta taaaagccgt tactaggagt aacagcagaa tcactctttt
     5581 catagtctct cccttcaagg tacagaaagg tatttatcct tccatagttg tgcgattaat
     5641 atctactgag ttcgttggcc tttctcacct gcttaaatcc aataaagacg attcccagaa
     5701 ggagcaatgc ccttaaaacc gattttgcaa caagcactag gtaggacgaa ctctcaaaca
     5761 gttcaggctc aatgactaat cccaagggaa ggagcattat tgccagcagg ggcattccat
     5821 tggacccttt cttccaggaa catatgatta tcacaatggc tacgactacc aggtaagcga
     5881 ccaggggtac ggttctaagg aaaagaaagg tcctgtccgg ttggagctga aaatgtccgt
     5941 tttgcggaaa aatcccgatt accaccctaa atacaagaag tccgtaaaga agcttgtcca
     6001 agggtacctt ttttcgcaga ccaaagcatt ccttgttcag atctatcagc attgaaaaaa
     6061 acagggtcat tacaataagg tggcccagcc taccccagcc aatgagattg atgttatcct
     6121 caagacctgt tgtaataagg gcatagattc tgggaagaaa gtatgtgctc tcccctatgg
     6181 caagggtcac tgcaagagac ccaaaggcca tgtggacctt gttgccattg tggtttctgt
     6241 atatgtatat tcccatgtag attagaattg ctatgtaaac caggaatccc aacggctcta
     6301 atatcttctg caagaaaatc cccccttggt ttgtgttcct aagttaagtt taatattaat
     6361 cacaggtatt gtaaatactt tcccccatat ttcatgcctt ggagtagttt atttttgttg
     6421 aaataggatt ggatttgtgc taatatatgt ttacatgggc ggttagttca gctggtagaa
     6481 cgctacgttg acatcgtaga ggtcgttggt tcgagtccaa tatcgcccac cat
//
LOCUS       DASRQF010000238         7819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_316773, whole genome shotgun sequence.
ACCESSION   DASRQF010000238 DASRQF010000000
VERSION     DASRQF010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 7819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7819
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316773"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /gene="pyrF"
                     /locus_tag="VFD79_06285"
     CDS             complement(<1..681)
                     /gene="pyrF"
                     /locus_tag="VFD79_06285"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HZH93691.1"
                     /translation="MIIDKLYERVREKGMVCVGLDSSLDYIPDEIKEKFQDVDQIIFQ
                     FNKRIIDATGDITAIYKPQIAFYEAYGLKGLAAYSRTLSYLRSEGHLSIGDIKRGDIS
                     STAKMYGKGHFEGDFETDFITINPYMGFDSITPYLPYVESGKKGLFVLLRTSNPGARD
                     IEYLEADGKKVYYHVGDGLARMAGDYMGGCGYSSIAAVVGGTHVDEAVEIRERYRDLF
                     FLIPGYGAQ"
     gene            complement(668..1888)
                     /locus_tag="VFD79_06290"
     CDS             complement(668..1888)
                     /locus_tag="VFD79_06290"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HZH93692.1"
                     /translation="MKLLIKGAHLIDGHHAFNKDIYIEDGIIEEIGEDLEHDCQWVDA
                     RGLVLLPAFVDIHAHFRDPGQPHKEDLMTGGMAALKGGYTFVNLMGNTDPVCSNMDTI
                     NHVREKARDLDLVEVHQCASITENLDGKTLDHLDGLDDNVRMITDDGKGVSSPLTMYR
                     AMEKVRERGMILMAHAEEMDLTEIDYRLSENLETVRDIYLAKHTGARLHLTHVSTREA
                     IDEIRRARSGGADKITCDVTPHHISLWDNDYRVNPPIREKEDVEALIEGIRDGTVDAI
                     GTDHAPHTQEDKEKGSPGISGIETAFGICHTHLVKAGHINMGQLVRLMAENPARILGI
                     NKGRLEKGYDGDLVLVDLDRESEINGERFVSKGKNTPFSGMKVYGEVLMTIRGGSIKY
                     MSKSCGEERLYDNR"
     gene            complement(1890..2318)
                     /locus_tag="VFD79_06295"
     CDS             complement(1890..2318)
                     /locus_tag="VFD79_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HZH93693.1"
                     /translation="MLNITSIKKGLVIDHIRPGMGLKIFEYLKLGKADFTVALIMNAS
                     SNKMGKKDIIKIEDVINVDYAMLGFLDANITVNVIENEVITEKINLSLPERVQDIIKC
                     KNPRCITTVERDITHKFVLIDKDKGIYKCEYCDHIYSWEG"
     gene            complement(2319..3242)
                     /gene="pyrB"
                     /locus_tag="VFD79_06300"
     CDS             complement(2319..3242)
                     /gene="pyrB"
                     /locus_tag="VFD79_06300"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002839082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HZH93694.1"
                     /translation="MLKGRHLVDPQDFSIQELESIFELTQEIINKPEDFNSICHGKIL
                     STLFYEPSTRTRFSFEAAMLRLGGGVIGFSEPGSSSVAKGESLSDTLRTVAAYADIIA
                     MRHPNEGAPKHGIQYCPVPLINGGDGGHQHPTQTLTDLFTILESKGRLDNLTIGCCGD
                     LKFGRTVHSLIKAMSRYENVKFILVSPKELQVPDYIRSEILEQRKIEFQEVERLEEVI
                     DQMDILYMTRVQKERFFNEADYVRLKDSYIVDNEKLKDAKEDLAILHPLPRVNEIAVE
                     VDQDPRAWYFRQVKNGMFVRMGLILKLLGVK"
     gene            complement(3426..4073)
                     /locus_tag="VFD79_06305"
     CDS             complement(3426..4073)
                     /locus_tag="VFD79_06305"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HZH93695.1"
                     /translation="MKYAIFDLDGTLLDSMWVWETLAYDYLKSRGIDVPDDIREKLRE
                     YSLREASDVLKEMYSLPESGEEINDQMEKVLEEYYFERIQLKEGARDLLQSLKDKGIG
                     MCAATATADHLARAAMERLEIDGYFDFLQTCKSTGIEKYKPEFYQLILDRVDAGPEDI
                     WVFEDALHSMEAAKKLGIKVAAIRDRSAEIDWEEIGKIADMIFDSFPEYLEKTSG"
     gene            4231..5673
                     /locus_tag="VFD79_06310"
     CDS             4231..5673
                     /locus_tag="VFD79_06310"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HZH93696.1"
                     /translation="MKHYLEKGGEVEVILVFKETAGVGTFNDLFGFIKDRDLFKGDAG
                     EVYSHISHEGDSVVFLGLGEEEKLQYDTLRKSFFSLGGELQKKKIKSIGLTVPKFGDR
                     CYGKTNKAIWEGLLQSQYDFDKFKSEKKPKVLLQDVYLDIVEGEAEKVESVIQETQFL
                     MEGVFLTRDLVNEPAINMYPEVLAKEAVDRLTPVGVDVKVLGRKEIQDLNMEAFLSVA
                     RGSSKEPKFIVMTYKGNPDSQEKLALVGKGLTYDSGGYSLKPAAGMATMHSDMAGSAS
                     VIGAMYSIAANKLKKNVVAIVAACENMVSGDAYKTGDIIGSMSGKTIEVENTDAEGRL
                     TLADALWYAATVEKADKIVDLATLTGACVVALGNINTGAITNDENLMGAVRTAAKLAG
                     EPVWQLPNDEEYKDLFKSHFADLKNTGGRGAGAITAGMFLQEFVNDIPWVHMDIAGTA
                     FLDKAAGYLPKGASGVPVKTLFYLAKDIDQ"
     gene            complement(5728..5901)
                     /locus_tag="VFD79_06315"
     CDS             complement(5728..5901)
                     /locus_tag="VFD79_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93697.1"
                     /translation="MLGGLVVGLVVGWIVSLFGGDVLIINGIQELTGNSISSAGYYTI
                     FAIIGLLGGVLKK"
     gene            complement(5963..6679)
                     /locus_tag="VFD79_06320"
     CDS             complement(5963..6679)
                     /locus_tag="VFD79_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93698.1"
                     /translation="MALWLAGCAGGTTGENPPLENGDMEALQQELEEKDAEIEALNSE
                     IAQLQEEIQELQSEPPEDPVPLSSGPTQGASLLTTAMEVIELIENMAMSDLDVYVHPT
                     KGVRFSPYDYVDLQNQVVIQAAQFQNLVNSPVVHNWGEFDGTGDPIDMTFDDYYDRFI
                     YDVDFANPEMIGNNYKIGMGNIINNVDTEYPNGQFVEFHFTGFDPQYQGMDWRSLKLV
                     FEDHNGAWHLVGIIHGEWTI"
     gene            complement(6824..7318)
                     /locus_tag="VFD79_06325"
     CDS             complement(6824..7318)
                     /locus_tag="VFD79_06325"
                     /inference="COORDINATES: protein motif:HMM:NF012481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HZH93699.1"
                     /translation="MKIGIVVHSMTGNTSLVAERIKNRLDQEGHELSLERLAIIGGED
                     QTQSDPDKISIEPLKMNIEELDLLILGGPVRGFQASPAMKALLGRLEGLEGKDTMIFV
                     TYAFPFNWMGGNSAIKQLRNMCTEKGANILFTGIIDWKNSKREVQIDSLAAGVARKAS
                     KAAS"
     gene            complement(7331..7717)
                     /locus_tag="VFD79_06330"
     CDS             complement(7331..7717)
                     /locus_tag="VFD79_06330"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HZH93700.1"
                     /translation="MKKRWKILLGILAVFVIAAVGMMMYLQTNLNKLMYEEIVEADLQ
                     NIEDGEYIGEYSSPPVNAKVQVEVMDGKIRDIVILEHGHGQGGPAEAIVDDVIAQQKI
                     DVDSISGATYSSRVILKAVEDALKDN"
BASE COUNT         1978 a         1996 c         1529 g         2316 t
ORIGIN      
        1 ctgggcacca tacccaggaa tcaggaagaa aaggtccctg tacctttccc ttatttcaac
       61 tgcctcatca acatgggttc cacccaccac ggcggctatt gatgaatatc cacatccacc
      121 catataatct cctgccattc gggcaagccc atcccccacg tggtagtaga ccttcttgcc
      181 gtcagcttcc aggtactcga tatcccttgc acctgggttg gatgtcctta aaagtacaaa
      241 cagtcccttc tttccagatt caacatatgg caggtagggg gttatgctgt caaagcccat
      301 atatgggttt attgtgatga aatccgtttc aaaatcaccc tcaaaatgac ccttgccata
      361 catcttcgca gtggaggata tgtcccccct cttaatgtcc cctatggaca ggtgaccctc
      421 tgacctaagg taggaaaggg tcctgctgta tgcagccagg ccttttaggc cataggcctc
      481 gtagaaggca atctgtggct tgtagatggc tgttatgtct cctgttgcat ctatgatcct
      541 tttattgaac tgaaatatta tctggtccac atcctggaat ttctccttta tctcatctgg
      601 gatgtaatcc aggctggagt ccagaccaac acaaaccatc cctttttccc ggaccctttc
      661 atacagctta tctattatca tacaatctct cctccccaca tgatttggac atgtatttta
      721 tgctgccacc tcttatggtc ataagaactt cgccatagac cttcatacct gaaaatggtg
      781 tgttctttcc ctttgataca aacctttccc cgttgatttc gctttcccta tccaggtcga
      841 caagaaccaa atccccatca taacccttct ccagtcttcc cttgtttatt cccagtatcc
      901 ttgcaggatt ctctgccatc agccttacaa gctggcccat attgatgtga cctgccttga
      961 ccaggtgggt gtgacagatc ccaaacgcag tctcaatccc ggagattcca ggacttccct
     1021 tctccttatc ctcctgggtg tgaggtgcat ggtcggttcc aatggcatca acagtaccat
     1081 cccttatgcc ttcaatcaag gcctcaacat cctccttttc cctgattgga ggattgaccc
     1141 tgtagtcatt gtcccacagg gaaatgtgat ggggtgttac atcgcaggtg atcttgtctg
     1201 cacctccgct tcttgccctc ctgatttcat caattgcctc ccttgtgctg acatgggtca
     1261 ggtggagcct ggcacctgtg tgcttggcca aataaatgtc ccttactgtt tcaaggtttt
     1321 cggaaagcct atagtcaatc tctgtcaggt ccatctcctc cgcatgggcc ataaggatca
     1381 tgcccctctc ccttaccttc tccattgccc tgtacatggt caagggagag cttaccccct
     1441 tgccatcgtc agtaatcatc cttacattat catccaatcc atccaggtga tcaagtgtct
     1501 tcccatcaag gttttcagtg attgatgcgc attgatgaac ctccacaaga tccaggtccc
     1561 ttgccttttc ccggacatgg tttatggtat ccatgttgga gcatacaggg tctgtgtttc
     1621 ccataaggtt cacaaatgtg tagcctccct taagggctgc cattccacct gtcataaggt
     1681 cctccttgtg aggctggcca ggatccctga aatgtgcgtg gatatcaaca aatgctggaa
     1741 gaaggaccag cccccttgca tccacccatt gacaatcatg ctccaggtcc tctccaatct
     1801 cttctatgat accgtcctca atgtaaatgt ccttattaaa agcatggtgc ccatcaataa
     1861 gatgtgcacc ctttattaaa agtttcattt tagccctccc aggaatatat atggtcgcag
     1921 tactcacact tatatatacc cttgtctttg tcaatcagta caaatttatg ggtaatgtcc
     1981 ctttccacag ttgttatgca tcttggattc ttgcatttta taatatcctg aaccctttca
     2041 ggcagggaaa ggttaatctt ttctgttatc acttcatttt cgattacatt aactgttatg
     2101 tttgcatcca aaaatcccag cattgcataa tcgacgttta ttacatcctc aatttttatg
     2161 atatcctttt tccccatttt attgctgctt gcattcatga tgagggcaac ggtgaaatca
     2221 gcctttccaa gcttaagata ctcgaatatc ttaagtccca ttcccggtct tatgtggtca
     2281 ataaccagtc cctttttaat gcttgttata ttcaacatct acttcacccc cagaagctta
     2341 agaataagtc ccattcttac aaacatgccg ttttttacct gtctgaaata ccaggctctt
     2401 gggtcctggt caacctcaac cgctatctca ttcacccttg gcaggggatg gagtatggcc
     2461 aggtcctcct tggcatcctt cagcttttca ttatccacga tgtagctatc cttcagtctt
     2521 acataatcag cctcgttaaa aaatctttcc ttctgaaccc ttgtcatata gaggatgtcc
     2581 atctggtcaa ttacctcttc aagcctttca acctcctgga actctatttt tctctgctca
     2641 aggatttcac ttctgatata gtcgggaacc tgaagctcct ttggtgatac aagtataaac
     2701 ttgacgtttt catatcgtga cattgccttt atcaaggagt ggacggtcct tccaaactta
     2761 aggtctccac agcagcctat tgtcaggtta tcaagtcttc cctttgattc caggattgta
     2821 aaaaggtctg taagggtctg ggttgggtgt tggtgcccac cgtctcctcc gttgatcagc
     2881 ggaactgggc agtactgtat cccatgcttg ggtgctcctt cgtttggatg cctcatggct
     2941 atgatatccg cataggctgc gactgtcctg agggtatccg aaagactttc tccctttgcc
     3001 acagaactag agcccggctc tgaaaacccg ataactcctc cacctaatct caacatcgcg
     3061 gcttcaaagc taaatctggt tctagtgctg ggctcataga acaatgtact gagaatcttt
     3121 ccatggcaaa tggaattgaa atcctcaggc ttattaataa tctcctgtgt cagttcaaaa
     3181 atactttcaa gctcctggat tgaaaaatcc tgggggtcta ccaagtgtct tccttttagc
     3241 ataaatattc ctccctttag tttaaataaa cctcaaggaa cagcgcatta aaaatgccct
     3301 ccacaaagga aggcacaagt atcctactta tacgctctcc tttccgatct cacgggatcg
     3361 atttaaaggt atttatttac ctaaagatat cacggagaga tgtataagtc aataattttt
     3421 ggggctcatc cagatgtttt ttcaaggtat tccggaaagc tgtcaaagat catgtctgca
     3481 attttcccta tttcttccca gtcaatctct gctgacctgt cccttatggc agcaaccttt
     3541 attccaagtt tttttgcagc ctccattgaa tggagggcat cctcaaagac ccaaatatcc
     3601 tcaggccctg catccaccct gtcaaggatc agctggtaga attcaggctt gtatttttct
     3661 atgcctgtgc ttttacaggt ctgaagaaag tcaaagtatc catcgatctc cagcctttcc
     3721 atggcagccc ttgcaagatg gtctgccgtg gcagttgcag cacacatgcc tattcccttg
     3781 tccttcaagg attgaagcag gtccctggca ccctccttaa gctggatcct ttcaaaatag
     3841 tattcttcaa gaaccttctc catctggtca ttgatctctt ctccactttc aggtaagctg
     3901 tacatttcct tcagtacatc cgaggcttcc cttagactgt actccctaag cttctccctt
     3961 atatcatcag gaacgtctat ccctcttgat ttaagatagt cataggccag ggtctcccat
     4021 acccacatgg aatcaagcag tgtcccgtcc agatcgaata tcgcatactt catggaattc
     4081 ctccttccca acctccgtct tcctttttct gtatacccta aaggtggtga tattcccaaa
     4141 aaacattttc cttttgggta ttttgtttgg tcacctgcca acaatggtat aatatcctaa
     4201 gagagtctaa atatatcagg aggtgtacgg atgaaacatt atctggaaaa gggtggagaa
     4261 gttgaggtga tattggtctt taaggagact gccggcgtag ggaccttcaa tgacctgttc
     4321 ggtttcataa aggataggga cctgtttaag ggagatgctg gtgaggtcta ctcacatatt
     4381 tcccatgaag gagattctgt tgttttcctt ggtctgggcg aggaggaaaa gctccaatac
     4441 gataccctta gaaagtcatt tttctccctt ggtggagagc tgcaaaagaa aaaaataaaa
     4501 agcattggat tgaccgtacc aaagttcggt gacagatgct acggaaagac aaacaaggcc
     4561 atttgggagg gtcttctcca atcccaatac gatttcgaca agttcaagag cgagaaaaaa
     4621 ccaaaggtac ttctccagga tgtctacctt gacattgtgg aaggtgaagc tgaaaaggtt
     4681 gaaagcgtaa tacaggaaac ccagttccta atggaaggtg tcttcctcac aagagacctt
     4741 gtgaatgaac cagctataaa catgtatccg gaggttcttg ccaaggaggc agtggacagg
     4801 cttactcccg ttggagtgga tgtaaaggtt cttggcagga aggaaatcca ggatcttaac
     4861 atggaagcct tcctttcagt agccaggggg tcttcaaagg agccaaagtt tatagttatg
     4921 acctacaagg gcaaccctga cagccaggaa aaacttgcct tggttggcaa ggggcttacc
     4981 tatgactcag gtgggtactc actgaagcca gctgcaggaa tggcaacaat gcactcggat
     5041 atggcaggaa gcgcttcagt tattggggca atgtactcaa tagccgccaa caagctgaag
     5101 aaaaacgtgg ttgcaattgt tgcagcctgt gaaaatatgg tctctggaga tgcatacaag
     5161 acaggtgaca ttataggttc aatgtcaggg aagaccatcg aggttgaaaa taccgatgca
     5221 gagggaaggc ttactcttgc ggatgccctt tggtatgcgg caaccgttga aaaggcggat
     5281 aagatagttg accttgcaac ccttacagga gcttgtgtag tggcccttgg aaacatcaac
     5341 acaggtgcaa taacaaatga tgaaaacctt atgggtgcag tcaggacggc tgccaaactt
     5401 gccggggaac ctgtatggca actcccaaac gacgaggaat acaaggacct cttcaagagt
     5461 catttcgccg accttaagaa tacaggaggc cgtggcgctg gtgcaatcac tgcaggcatg
     5521 ttcctgcagg aatttgtcaa tgatatccct tgggtccaca tggacattgc aggaaccgca
     5581 tttcttgaca aggcagctgg ctaccttcca aagggagcct ccggtgttcc ggtcaagacc
     5641 ctcttctacc ttgcaaagga tattgaccaa taaaccattt aaaagatgcc tgattctcgt
     5701 gttgagaatc aggcatttct aattaagcta ttttttcaag actcctccga gaagccctat
     5761 tattgcaaat attgtgtaat agccggcgct ggagatagaa tttcccgtaa gctcctgtat
     5821 tccattgata atcagtacat ctcccccgaa tagggatact atccaaccta cgaccaatcc
     5881 tactactagt cctcctaaca tacacacacc ccctaaacag tttatcctct cttatgatac
     5941 cagatttgcg aggataaatc cattaaatgg tccactcacc atggattatt ccaaccagat
     6001 gccaagcacc attgtggtct tcaaatacca gcttcaggct tctccaatcc attccctggt
     6061 actggggatc aaagcctgtg aagtggaatt ccacaaattg accgtttggg tactctgtat
     6121 caacgttatt tattatattg cccattccaa tcttgtaatt gtttcctatc atttcagggt
     6181 ttgcaaaatc aacatcatag atgaacctgt cgtaatagtc atcaaaggtc atatcaatcg
     6241 ggtcccctgt tccgtcaaac tcaccccagt tgtggactac cggactgttt accaggttct
     6301 ggaattgggc agcctgtata acaacctggt tctgaagatc cacataatca tatgggctga
     6361 atctgactcc ctttgtcggg tggacgtaaa cgtccaaatc tgacatggcc atattctcaa
     6421 taagctcaat tacctccatg gcagttgtaa gcagtgatgc tccttgtgtg gggcctgatg
     6481 acaatggtac cggatcctca ggtggttctg actgaagctc ctgaatttcc tcctgcagct
     6541 gtgctatttc agaattcagg gcctcaatct cagcatcctt ttcctccaac tcctgctgta
     6601 atgcctccat gtctccattc tccaaaggtg ggttttctcc tgtcgtccca ccagcacagc
     6661 ctgccagcca taatgccatc actaatatca aaacggtcca taactttcct ttaccggtca
     6721 taatctcaca tcctttactt gtttgatcta ttcctaccct ttttctaggt ttttaatatt
     6781 tcaatgtagt tacgatgtta ggttatgccc gttattgaag tgactatgac gcagcttttg
     6841 aagcttttct tgcaactcca gcggcaaggc tgtcaatctg tacctccctt tttgaattct
     6901 tccagtcaat tattcccgtg aagagaatat ttgcgccttt ttcagtacac atatttcgaa
     6961 gctgcttgat ggcactgttt ccacccatcc agttaaaagg aaatgcatag gtcacaaata
     7021 tcatagtatc ctttccctca agaccctcta gtctgcctaa aagtgccttc atggccggag
     7081 aagcctggaa tccgcgaaca ggtccgccca ggatcaatag gtccaattct tcaatattca
     7141 ttttcaaggg ttcaatactg attttatccg ggtccgactg ggtctggtcc tcaccgccaa
     7201 ttattgccag tctttccagg gaaagttcat gtccctcctg gtccagtctg ttcttgattc
     7261 tttctgccac aagagaagtg ttcccggtca tagagtgtac aactatacca attttcatcc
     7321 tttacccccc ttaattatcc tttagggcat cctctacagc cttcaaaata accctgctgc
     7381 tgtaggttgc acctgatatt gagtccacgt cgatcttctg ctgtgcaatt acatcatcaa
     7441 caatagcctc tgccggtcct ccctgcccat gtccatgttc aagtattaca atatccctta
     7501 tttttccgtc catcacctca acctggacct ttgcgtttac aggtggtgat gaatattctc
     7561 ctatgtactc accatcctca atattctgaa ggtcagcctc tacaatttcc tcatacatga
     7621 gcttattcaa atttgtctgc aggtacatca tcattcccac cgctgcaatt acaaacacag
     7681 ccaggattcc aagtagtatc ttccatcttt ttttcacgtt ctcactccca attatacatt
     7741 tcacgttaaa gtattatcaa gtagattata ccactaaaaa acccagatgc aattcacaat
     7801 tcataattca caatgcaca
//
LOCUS       DASRQF010000239         3157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1613780, whole genome shotgun sequence.
ACCESSION   DASRQF010000239 DASRQF010000000
VERSION     DASRQF010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613780"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..960)
                     /locus_tag="VFD79_06335"
     CDS             complement(16..960)
                     /locus_tag="VFD79_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZH93701.1"
                     /translation="MRKGLIIIMVAALFLLGGCGEGGLPEEDYNLEVAEGEIKGSLML
                     PEGEGPFKLAIIHQGSGPTDRDGNSAIAGDNNSLKMVAEDLAQAGIASLRFDKRGIAK
                     SMSLIEREEDLVFEDYVYDMILWIEKTEQDERFSEIYLIGHSEGALISAAAAAQKEVA
                     GFVSLAGVGERASDTLRRQLSGQPEEITSMTNPIIGELEQGRLVDNVPEVLQSLFRDS
                     IQPYLISWFKYDPTEVYGEISSPIMVIQGDNDVQVTVNDAMILSQAAGTKPVLISGMN
                     HILKDAPRDMEGNLETYKDPDLELHPDLMPEIIRFIND"
     gene            complement(1019..2395)
                     /locus_tag="VFD79_06340"
     CDS             complement(1019..2395)
                     /locus_tag="VFD79_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HZH93702.1"
                     /translation="MTKYYAEPFKIKMVEPIKILTREERIEKIKEANYNVFALDSDDV
                     YIDLLTDSGTGAMSADQWAGIMVGDEAYSGSRSFKNLMASAKDVFGYKYFQPVHQGRA
                     AEKVVMPIFLHEGKVAISNMHFDTTRAHVELAGARAIDCVIPEALDTVNFHPFKGNMD
                     NVRLKKLIEENGPENVGMVIITVTNNSAGGQPVSMENIRETSEIAKSYGIPVCIDAAR
                     YAENAYFIKMREEGYKDKPIKEIAQEMFSYADVFTMSAKKDAIINMGGLIGVKDDPDL
                     YEMVKARTIPYEGFISYGGLSGRDIEAMAIGLQEGTSVDFLRYRIGQMEYLAGKLDEE
                     GIPYQSPVGGHAVFLDAKQLLPHIPYHEFPGQALAIELYIEAGIRSCDIGSYMMGNDP
                     DTGEQIESQFEFTRLAVPRRVYTQSHLDVIVEALKEIKARKDQVKGYEITWEPKVLRH
                     FTAKLKPL"
     gene            complement(2420..2998)
                     /locus_tag="VFD79_06345"
     CDS             complement(2420..2998)
                     /locus_tag="VFD79_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZH93703.1"
                     /translation="MSDYKPTREEAYELLTKYVENDSQIKHALAVEATMVHFAQLYNE
                     DPEKWGVIGLIHDLDYDKYPEEHCKVTEQILKEEGWPEDYIRGVLSHGWKLCTDVEPR
                     ETMEKVIYTIDELTGLVAATALMRPSKSIMDMEAKSVKKKWGQKSFAAGVNRDVIAEG
                     AEMLGMDLKEVIHETIMGMRKVADEIGLKGDL"
BASE COUNT          725 a          846 c          573 g         1013 t
ORIGIN      
        1 aacattatcc cgtttctagt cattgataaa ccggattatt tcaggcatca ggtccggatg
       61 gagctcaagg tcaggatcct tgtaggtttc aagatttccc tccatatccc ttggagcatc
      121 ttttaggatg tgattcatgc cagatatgag gacgggcttt gtacccgctg cctgtgaaag
      181 aatcatggcg tcattcactg ttacctgtac gtcattgtct ccttgaataa ccataattgg
      241 tgaggatatt tccccatata cctccgttgg gtcgtacttg aaccaggata tgagatatgg
      301 ctggatgcta tccctgaaga gtgattgcag gacctcaggc acgttgtcta caagtctccc
      361 ctgctcaagc tcccctatga tgggattggt catggatgtt atttcctcag gctgtcctga
      421 cagctgcctt ctcaaggtat cagatgccct ctctccaact cctgccaggg atacaaagcc
      481 cgcaacttcc ttctgggctg cagctgcagc tgagatcagt gccccttcac tgtgaccaat
      541 cagatatatt tcgctgaatc tttcgtcctg ctcagtcttt tcaatccaca aaatcatatc
      601 gtatacataa tcctcaaata caaggtcctc ttccctttct atcaaggaca tggacttggc
      661 gattcccctc ttgtcaaaac gcagggatgc aattccagcc tgggccaggt cttctgccac
      721 catcttaaga ctattgttgt cccctgcgat agcactgtta ccgtccctat cagttgggcc
      781 agacccctga tgaattattg caagcttaaa tggtccttct ccctcaggga gcatcagtga
      841 ccctttgatc tcaccttccg caacctccag gttatagtcc tcctctggaa gccccccttc
      901 tccacagcca ccaagcaaaa ataatgctgc taccataatt ataatcaaac cctttctcat
      961 gagttttccc ccttacatag ataggacagg agagccttta ctctcctgtc ctcaactcct
     1021 aaagtggctt aagctttgca gtgaagtgtc tcaggacctt aggctcccag gttatttcat
     1081 aaccctttac ctgatccttc ctagccttga tctcctttag tgcctctact attacatcaa
     1141 ggtgagactg ggtgtaaacc cttcttggta ctgcaagtct tgtaaattca aattgtgatt
     1201 ctatctgttc tcctgtgtca gggtcatttc ccatcatgta tgaaccgatg tcacagcttc
     1261 taatcccagc ttcaatataa agctctattg ccagagcctg ccctgggaac tcatggtatg
     1321 gaatatgtgg cagcagctgc tttgcatcta ggaagacagc atggcctcca actggtgact
     1381 ggtatggtat cccttcctca tccagcttgc cagcaaggta ttccatttga cctattctgt
     1441 atcttagaaa gtcaacgctt gttccttcct gaagacctat ggccattgct tctatatccc
     1501 tacctgaaag ccctccatag gaaatgaagc cttcataagg tatggttctt gccttaacca
     1561 tttcatacag gtctgggtca tccttgactc ctataagccc tcccatgttg atgattgcat
     1621 ccttctttgc actcattgtg aatacatcag catagctgaa catttcctgg gcaatttcct
     1681 tgatgggctt gtccttatag ccttcttccc tcatttttat aaaatatgcg ttttctgcat
     1741 atctggctgc atcaatgcaa acaggaatac catatgattt ggctatttca gaggtctctc
     1801 ttatgttttc cattgagaca ggctggcctc cggcactatt gttggttact gttataatga
     1861 ccatgccaac gttttccgga ccattctcct caatcagttt ctttagtctt acattgtcca
     1921 tgtttccctt gaaaggatgg aagtttacgg tatcaagagc ttcaggtatt acacagtcta
     1981 ttgcccttgc tccagctagc tccacatggg cccttgtggt atcaaaatgc atgttggata
     2041 ttgccacctt gccctcatga aggaaaattg gcattacaac cttctcagca gctcgtccct
     2101 ggtgtacagg ttgaaaatac ttgtagccaa atacatcctt agcagatgcc atcagattct
     2161 taaagcttct ggagcctgag taggcctcat ctccaaccat tattccagcc cattggtcag
     2221 cgctcatggc gccggtccca ctgtctgtta aaaggtcaat gtaaacatcg tctgaatcca
     2281 gggcaaacac gttgtaattt gcctccttta tcttttcaat cctttcttct cttgtcaaaa
     2341 tcttgatcgg ttctaccatc ttaatcttaa acggttcagc ataatacttt gtcatctttt
     2401 cccctcctgt tttatatgat taaaggtctc ccttcagtcc gatttcatct gccacttttc
     2461 tcattcccat gatggtttca tggataactt ccttaaggtc cataccgagc atttccgccc
     2521 cctcagctat aacatccctg ttcacgccag ctgcaaagga cttctgtccc cacttcttct
     2581 tgactgactt ggcttccatg tccattattg acttgctggg tctcatcagg gctgtagctg
     2641 ccaccaatcc tgtaagttca tctattgtat agattacctt ttccatggtt tccctgggtt
     2701 caacatctgt acaaagcttc cagccatggc ttaatactcc tctgatataa tcctcaggcc
     2761 agccttcctc ctttagaatc tgctctgtta ccttgcagtg ttcctctgga tacttgtcgt
     2821 agtccaggtc gtgaataaga cctattacac cccatttttc agggtcctca ttatacagct
     2881 gggcaaagtg aaccattgtt gcttcaactg ccaaggcatg cttgatttga ctatcattct
     2941 caacatattt cgttaacagc tcatatgctt cctctcttgt tggcttgtaa tctgacacat
     3001 aatcccttct ttctttattc tattctatta tcttatttta cataattttc tcacaaaaga
     3061 caagaactcc ttgttcatta cttcagtttc ttgggatttc aaaccagaat aggctaccct
     3121 ctcccaggct gctttccaca cccaactgac cctggtg
//
LOCUS       DASRQF010000240         3934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_951540, whole genome shotgun sequence.
ACCESSION   DASRQF010000240 DASRQF010000000
VERSION     DASRQF010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951540"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..618
                     /locus_tag="VFD79_06350"
     CDS             73..618
                     /locus_tag="VFD79_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HZH93704.1"
                     /translation="MNAMTQDNLRSAFGGESQAHMRYRIWADKANKEGFPTVARLFMA
                     TSDAEQVHATLHFKALRDVHGAFSVVSGGEFGLGTTSENLAGGIEGEMHEVTQMYPAY
                     IQVAEMQGEKEAINAMRYAVAAEQVHADCYTLAKKFVDHGKDLEAAVIELCPVCGYIT
                     LTGEEESCPICNAKKSVFVAY"
     gene            673..1221
                     /locus_tag="VFD79_06355"
     CDS             673..1221
                     /locus_tag="VFD79_06355"
                     /inference="COORDINATES: protein motif:HMM:NF036410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 domain-containing protein"
                     /protein_id="HZH93705.1"
                     /translation="MIIETLLLAIAYGKLRGGKIGNISDLAIRMWGFIPAGFLLSYVS
                     IYLITRGNGFLFDNFVYIQLASNLLLLTTLFFNRSFWPFNLVSAGIMMNSIPMTLNGG
                     RMPVSGWALEKAGLIQELSLIAEDRIVTHTLIRDETFFMLLSDVIPLLYKVISIGDVL
                     ISLGIFLIIHKYMTSSQPLQRR"
     gene            1267..2499
                     /locus_tag="VFD79_06360"
     CDS             1267..2499
                     /locus_tag="VFD79_06360"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HZH93706.1"
                     /translation="MKKWIGISALIVLLVLIFTGMFIQGDMGVVDSVGGSNPGENVTS
                     QPDALDDSFTQTPPLETDKVERIEYSTLKIIAAGDLMFHMPQINSAKQADGSFNFFPP
                     FEHVTEYIQEADMAIANLETVTGGDHLGFSGFPRFNSPEAVLDAIAEAGFDVLVTSNN
                     HTLDRGKKGIINTIDKIKDRGLEYIGTSKDTRRPYIVEDRNDIKVGILSYTYGLNGLD
                     SLLTQEERSNMVNLIDKDLIREDIRALEVEEVDLVMAYLHWGYEYHSNPSQDQIELAE
                     FLSHSGVDIILGTHPHVVQKVDIIHTDSKMTLVVYSMGNFISNQRYDTMGVSATEDGM
                     MIQIEVEKDVVSGNTHVKTLRMIPTWIHRSWQAGAYEYRILPVEAALEGELDMDLDPA
                     TVKRLERSLSETTKALGD"
     gene            2622..3455
                     /gene="cdaA"
                     /locus_tag="VFD79_06365"
     CDS             2622..3455
                     /gene="cdaA"
                     /locus_tag="VFD79_06365"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HZH93707.1"
                     /translation="MQFLSDLFMNIRIRDIVDILIVAVVLYKLYTLIKETRAEQLLKG
                     IGVLLVLTEVSEWLELYTINWILGNAMTVGTLAILIVFQPELRRGLEYIGRSRFFSKS
                     LIEIRGESMSRVVDEIVEATASLARQKIGALIVLEKQTGLNEVVETGTKIGGTVSSDL
                     LINIFIPNTPLHDGAVIIKDDIIKAAACFLPLTDSSSVSKELGTRHRAALGISERSDS
                     ISIIVSEETGAISIAENGTISRYLDSKTLKQILLEMYKPKNSDQTFMTRWRNRDEDGK
                     K"
     gene            3436..>3934
                     /locus_tag="VFD79_06370"
     CDS             3436..>3934
                     /locus_tag="VFD79_06370"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HZH93708.1"
                     /translation="MKMGKSDVTAKILTLVIAIFLWSYVMSETDPVQTQEYRNVTVSY
                     TNTAALDRQGLVIMDPEEVKIDVRVTGKRSDFSQFSSSKIYAQVDLSGYSEGQVKIPV
                     TVGLLDQSSGIRVINHEPKEVLFTFDRIVSREIPVTMDYQGTLPENYVRGDTVSRPQS
                     ILVSGP"
BASE COUNT         1217 a          739 c          970 g         1008 t
ORIGIN      
        1 atgtaaattg taatgatttc acttttcaag ggtgaatcag gataagcaaa gacaaataaa
       61 ggaggaattt caatgaatgc aatgacacaa gacaacctga gatcagcttt tggtggagag
      121 agccaggcac atatgagata cagaatatgg gctgacaagg ctaacaagga agggttccca
      181 actgtggcaa gacttttcat ggcaacttca gatgctgagc aggtacacgc aacactccat
      241 ttcaaggccc ttagggatgt acatggagct ttctcagtag tatcaggagg agagtttgga
      301 cttggaacca catcagaaaa tcttgcaggt ggaattgagg gtgaaatgca cgaagtaacc
      361 cagatgtacc cagcttacat acaggtggca gagatgcagg gagagaagga agcaatcaat
      421 gcaatgagat atgcagttgc agctgagcag gttcatgctg attgctacac attggcaaag
      481 aaattcgtag accatggaaa ggaccttgag gctgcggtta ttgagttgtg tccagtatgt
      541 ggctatatca cccttacagg tgaagaggag tcatgtccaa tctgcaacgc taaaaaatca
      601 gtatttgtag cttactaatc aggacgccct cgggcgtcct attataatac caaaaggcag
      661 ggagtccata tcatgatcat tgaaacctta ttgttggcaa ttgcatacgg aaagcttaga
      721 ggtgggaaga ttggaaacat ttcagacctt gccataagga tgtgggggtt catacctgca
      781 ggctttctat tatcctacgt ctccatttac ctcatcacta gggggaatgg gtttttattt
      841 gacaattttg tctacataca gctggcatca aatcttctac ttttgaccac cctgtttttc
      901 aataggagtt tttggccctt caatctggtg tctgcaggaa ttatgatgaa ttccataccc
      961 atgacactta atggtggaag gatgcccgta agtggatggg cccttgaaaa agcgggtttg
     1021 attcaggagc tttctctcat tgcagaagat cggatagtga ctcatacatt gataagggat
     1081 gaaaccttct ttatgctcct ttcagatgtg atccccctgc tctataaagt catcagtatt
     1141 ggggatgtct taatatcatt aggtatattt ctgataattc acaaatatat gacttcatcc
     1201 cagccattgc agagaagatg atttagatgt aagatataga taagggcaga tgagagagga
     1261 attaaaatga aaaaatggat cggaatttca gcactcattg tattattggt attgatattt
     1321 accggcatgt ttattcaggg cgacatgggt gtcgtagact ccgttggtgg aagtaatcct
     1381 ggtgaaaacg ttaccagtca accagatgct cttgatgact catttacaca gactcctcct
     1441 cttgaaactg acaaggtgga gaggatagaa tacagtacat tgaaaataat tgcagcaggg
     1501 gatttgatgt ttcacatgcc tcaaatcaat tctgcgaaac aagctgatgg gtccttcaac
     1561 ttttttcctc cctttgagca tgttaccgag tacatacagg aggctgacat ggcaatcgct
     1621 aatcttgaaa ctgtaacagg aggggaccat cttgggttca gtggcttccc gcggttcaac
     1681 agcccggagg ccgtactgga tgcaatagct gaggcaggat ttgatgtgct tgtaacatca
     1741 aacaaccaca cccttgatag gggcaagaaa ggaatcataa atacaattga taagataaag
     1801 gatcggggat tggagtacat tgggaccagc aaggacacaa gaagacccta catcgtggag
     1861 gacaggaacg atataaaggt cggcatcctt tcctatacct acggtctaaa cggactggac
     1921 tcgctcttaa cacaggaaga gagaagtaac atggtgaatc tcattgacaa agatctcatc
     1981 cgggaggata ttcgtgccct tgaggtggag gaggttgacc ttgtcatggc atatttgcat
     2041 tggggatacg agtatcactc aaatccttct caggaccaga tagaacttgc ggaatttcta
     2101 tcccatagtg gtgtggatat aatactgggg acccatcccc atgtagtaca aaaagttgac
     2161 atcatccaca cagactcaaa aatgaccctt gtagtatatt ccatggggaa ttttatttcc
     2221 aatcaaaggt atgacacaat gggagtttct gcaactgaag atgggatgat gatccagatt
     2281 gaggtggaga aggacgtggt tagtggaaac acccatgtaa aaactcttag gatgatcccc
     2341 acctggatac atagaagctg gcaggctggt gcctatgaat acaggatcct acctgtggaa
     2401 gcagcactcg aaggggagtt ggacatggat cttgaccctg cgacagtaaa aaggctggaa
     2461 aggtcattgt cagagactac aaaggcactg ggagactgat tatttaaaac aaccgagttt
     2521 ccgaacatga gaatcaaaaa gcttccttaa atacatcttc aaatgcatag tcttataggg
     2581 tataatagga tgtagagact aaacaaaaag gcggggttcc attgcaattt ctaagtgatt
     2641 tgttcatgaa catcagaatt agggatatag ttgatatcct gatagttgca gttgttctat
     2701 acaagctgta tacattgata aaggaaaccc gtgctgagca gctattaaag ggcatagggg
     2761 ttcttttggt gcttactgag gtcagtgaat ggcttgaact atacaccatc aactggattc
     2821 ttggtaatgc catgacagtt ggaacacttg caatcctgat cgtttttcag cctgaactga
     2881 gaaggggatt ggaatacata ggtaggagca gatttttctc caaatccttg atagagataa
     2941 gaggggaaag catgtcaagg gtagttgatg agatagtgga ggctacggct tccctggcca
     3001 ggcagaaaat tggggccttg atagtgttgg aaaagcaaac tggactaaac gaagttgtgg
     3061 agacaggaac aaaaattgga ggaacagtca gcagtgacct tcttatcaat atttttattc
     3121 ccaacacccc cttgcatgac ggtgcagtaa taataaagga cgatatcata aaggcggcag
     3181 catgcttttt gcccctgact gacagctcat cagttagcaa ggaacttggt acaagacata
     3241 gggctgcatt gggaatctca gaaaggtctg acagcatttc aatcatagtt tcagaggaga
     3301 cgggagccat ttccatagca gaaaacggca caatatcaag atatcttgat tcaaagacct
     3361 tgaaacagat acttctggag atgtacaagc caaaaaacag tgaccagaca tttatgacaa
     3421 gatggaggaa tagagatgaa gatgggaaaa agtgatgtaa cagcaaaaat cctcactctt
     3481 gtaattgcca tattcctttg gagctatgtc atgagtgaga ccgatcctgt ccagacccag
     3541 gaatacagga atgtaacggt cagctataca aatacggcag ctttggacag gcaaggcctg
     3601 gtgatcatgg atcctgaaga ggttaagata gatgtgaggg taaccgggaa aaggtctgat
     3661 ttcagtcaat tcagctcatc caagatatat gcacaggttg atctgagcgg ctacagtgag
     3721 ggccaggtta agattccagt tacagttggt cttctggacc agtcatctgg gattcgggtt
     3781 ataaaccatg agcccaagga agtactcttt acctttgaca ggatagtgag cagggaaatc
     3841 ccagtaacaa tggattacca gggaacccta cctgagaatt atgttcgggg agatacagta
     3901 tcaagaccac aaagtatcct ggtaagcggg ccaa
//
LOCUS       DASRQF010000241         5227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1379177, whole genome shotgun sequence.
ACCESSION   DASRQF010000241 DASRQF010000000
VERSION     DASRQF010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379177"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFD79_06375"
     CDS             <1..342
                     /locus_tag="VFD79_06375"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004832798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HZH93709.1"
                     /translation="IEEPEKFGRSLVLQLLPGTQGLYGFVIGLLVLGQLDAGMTLAHG
                     MYLLAACLPIGFVGWQSAIAQGKVAAAGISILAKNEEHSTKGIIYSVMVETYALLAFV
                     ISLILVNAVSF"
     gene            364..921
                     /locus_tag="VFD79_06380"
     CDS             364..921
                     /locus_tag="VFD79_06380"
                     /inference="COORDINATES: protein motif:HMM:NF014093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HZH93710.1"
                     /translation="MSNLDNLTQKILEDAQNKASEIMEEAEKRNRSLVSHRVKEANER
                     RDKIVEKAAAEAQMLKSRVISSAELKVRDEKLTAKQEVMDKVFQMAKDKLSNLDNDDY
                     LEFVKASLENTKTEGKPTLLVPKGKKDQLEELSTAVELVEDPSVQSGYILKDGEIVYN
                     YTFEALVDEIREDLEGEIAQKLFEE"
     gene            932..1933
                     /locus_tag="VFD79_06385"
     CDS             932..1933
                     /locus_tag="VFD79_06385"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF014094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HZH93711.1"
                     /translation="MDRMEFTQGVTRTRVLETKLLDQLTIDKMVDAKDLEEVLRTLGD
                     TDYAGAVSAVEKGEDYESILSHELKRSYDLMREISPRDEVVDLMALKYDYHNLKVMLK
                     EHVMNKDLSDLYVQVGTVDFNKLKAMFKAEQMRDIQPELKAAIEEILDDYDQLEDPQR
                     IDILADRHYFKHLYRIADKTGIELFKAYVKDYIDFLNVSAAIRLKKQGKDLRFFREVI
                     LFNGNIERDDILLTMNDSIDVMMNKFRHARVSKDLLKGLQAYQETGRLSAFEKYMDNY
                     MINLNKDSKSITFGPEPLFSYLVAKEMEIKILRIIMVSKTNNLSPEAIRERLRDLYV"
     gene            1926..2234
                     /locus_tag="VFD79_06390"
     CDS             1926..2234
                     /locus_tag="VFD79_06390"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004832801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HZH93712.1"
                     /translation="MFKVGVVGDKDSVLAFKALGVDVFTVVDEEEARKTVDTMARSNY
                     GVIFITEQVASLIPDTVDRYDKEIIPAVILIPSNQGTLNIGMDRINENVEKAVGSNIL
                     "
     gene            2251..4014
                     /locus_tag="VFD79_06395"
     CDS             2251..4014
                     /locus_tag="VFD79_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HZH93713.1"
                     /translation="MKVGKIVKVSGPLVVAEGMDEANVYDVVQVSDFKLVGEIIEMRG
                     DRASIQVYEETTGIGPGDPVYTTGEPLSVELGPGMMEAMFDGIQRPLNKFREISGDFM
                     TRGTKVDNLDREKKWDFVPAVKVGDKVESGDVIGTVQETTLILHKIMVPPGMSGEVTE
                     IKEGRFTVVEDICVLDGEKRLQMMQKWPVRKGRPFAKKLNPDVPLLTGQRVIDTFFPV
                     AKGGTAAIPGPFGSGKTVVQHQLAKWADTEIVVYVGCGERGNEMTDVLMEFPEIIDPK
                     TGESLMKRTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYSVAIMADSTSRWAEAL
                     REMSGRLEEMPGDEGYPAYLASRAAAFYERAGKVICEGGDKREGAITVIGAVSPPGGD
                     ISEPVSQATLRIVKVFWGLDHALSYKRHFPAINWLNSYTLYQTKMDQWMAGNIDPEFA
                     KNRAKAMNLLQEESSLQEIVRLVGRDSLSEEDQLKLEATKSLREDYLQQNAFHDIDTF
                     CSLEKQNKMLKLVLQFYDLGMKALEKGVYLKDIEHMPIRENIARSKYIDEEELHKIDE
                     IGVELNQEINRLIEEGGILDA"
     gene            4007..>5227
                     /locus_tag="VFD79_06400"
     CDS             4007..>5227
                     /locus_tag="VFD79_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004832804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HZH93714.1"
                     /translation="MRKEYNTVREVVGPLMVVDGVEGVKYEELVDIELGTGEKRRGRV
                     LEVNGDKAMVQLFEGSSGINLGQTRVKFLGKPLELGVSEDMIGRIFDGLGRPKDDGPK
                     IIPEKKVDINGVPINPVSRDYPEEFIQTGISCIDGLNTLVRGQKLPIFSGSGLPHGEV
                     AAQIARQAQVLGTDENFAVVFAAMGITFEEAQFFIEDFTRTGAIDRAVLFVNLADDPA
                     IERIATPRMALTCAEYLAFEKDMHVLVILTDLTNYAEALRETSAARKEVPGRRGYPGY
                     LYTDLSTIYERAGRIRGRKGSITQIPILTMPEDDITHPIPDLTGYITEGQIILSRALY
                     KKGIEPPINVVPSLSRLKDKGIGDNKTREDHADTMNQIYAAYTSGIEARELATILGES
                     ALSDADKAFAKFAEA"
BASE COUNT         1595 a         1034 c         1411 g         1187 t
ORIGIN      
        1 atcgaggagc ctgagaagtt cggtcgttca cttgtacttc agctgctacc aggaacacag
       61 ggtctttacg gctttgtaat aggacttttg gttcttggac agttggatgc gggcatgact
      121 ctggctcatg gcatgtatct ccttgcagcg tgtttgccaa tcggttttgt tggatggcag
      181 tcagccatag cccagggaaa ggttgctgct gccggtattt caattcttgc aaagaacgaa
      241 gagcattcaa caaagggtat catctactca gtaatggttg agacatatgc cctgctggca
      301 tttgtaatct cactaattct tgtaaacgcc gtaagtttct agaaaaagta aggatgtgaa
      361 gctatgtcaa atttagataa ccttacccaa aagattcttg aggatgccca gaacaaggca
      421 agtgagatca tggaagaggc tgaaaaaagg aacaggagtc ttgtcagcca tagggtaaag
      481 gaagcaaatg agagaaggga taagatcgtc gaaaaggctg ccgccgaggc acaaatgcta
      541 aagagcagag tgatctcaag tgctgaattg aaggtcaggg acgaaaagct tactgcaaag
      601 caggaggtta tggacaaggt ttttcagatg gcaaaggaca agttgtcaaa ccttgataat
      661 gatgattacc tggaatttgt aaaggcttct cttgaaaata caaagacaga aggcaagccc
      721 acacttctgg ttccaaaggg aaaaaaggac cagctggaag aactttccac ggcagtggag
      781 cttgtggagg acccttcagt ccaatcaggc tatatactta aggatgggga aatagtttat
      841 aactatacat ttgaagccct ggtggatgaa atcagggaag acctcgaagg ggagattgca
      901 cagaaactct ttgaagaata ggagtgagtt catggataga atggaattta cccagggggt
      961 aacccgtacc agagtccttg aaaccaagct ccttgaccaa ttgaccatcg acaagatggt
     1021 tgatgcgaag gatcttgagg aggtactgag aacccttgga gatacggatt atgcaggtgc
     1081 ggtgtcagct gtggaaaaag gagaggacta cgaaagcatc cttagccatg agctgaaaag
     1141 gtcatacgac ctcatgaggg agatttcacc ccgtgacgag gtggtggacc tgatggccct
     1201 gaagtatgac taccacaacc ttaaggtgat gcttaaggaa cacgtaatga acaaggacct
     1261 atccgatttg tacgttcaag tgggtacggt tgatttcaat aaactcaagg ccatgttcaa
     1321 ggccgagcag atgagggaca tacagcctga attaaaggct gcaattgaag aaatcctgga
     1381 tgattatgac cagcttgagg atccccagag gattgacatc cttgcggaca gacactattt
     1441 caagcatctg tacagaattg cagataaaac gggaatagaa ctgttcaagg cctatgtgaa
     1501 ggactacata gattttctca atgtatccgc tgcaatcagg ctaaagaagc aaggcaagga
     1561 cctcaggttc ttcagggagg tgatcctctt caatggaaac attgaaaggg atgacatact
     1621 ccttaccatg aacgattcaa ttgatgtgat gatgaacaag ttcaggcatg ccagagtaag
     1681 caaagacctt ctcaagggtc ttcaggcata tcaggagacc ggtaggctgt ccgcatttga
     1741 gaagtacatg gacaactaca tgataaacct taacaaggat tccaaatcca taacattcgg
     1801 acctgagccc ctcttttcat atctggtggc caaggaaatg gaaattaaga tactaaggat
     1861 cataatggtt tctaagacga acaatctttc accggaggcc ataagagaaa ggttgcgtga
     1921 tctatatgtt taaagttgga gtagttggag acaaggattc cgttctggcc ttcaaggcat
     1981 tgggtgtaga tgtctttacc gtggtggatg aagaggaagc cagaaaaacc gtggatacaa
     2041 tggccagaag taattatggc gttatcttta tcactgagca ggttgccagt ttgataccag
     2101 atactgttga tagatatgac aaagagataa tccctgcggt tattcttatc cctagcaacc
     2161 agggaaccct gaatataggg atggacagga taaacgagaa tgttgaaaag gccgtaggat
     2221 caaatatttt atagaaagcg agggcttatc ttgaaagtag ggaaaatagt aaaagtatct
     2281 ggacccttgg tagttgctga ggggatggat gaagcgaatg tatatgacgt agtacaagta
     2341 tccgacttca agcttgtcgg tgagataata gagatgagag gcgacagggc atcgatccag
     2401 gtttacgagg agactacggg aattggtccc ggggaccctg tgtacaccac tggcgagcct
     2461 ttgtcagttg agcttggacc tggtatgatg gaggccatgt tcgatggtat ccagagacct
     2521 ctgaacaagt tcagggaaat atccggagac ttcatgacca ggggaacaaa ggtcgacaac
     2581 ctggacaggg agaaaaaatg ggactttgtt ccggccgtca aggttggaga caaggttgaa
     2641 tctggagatg taataggtac ggtccaggag accacactta ttctacacaa gataatggtt
     2701 cctcctggaa tgtcaggtga ggtaactgaa ataaaggaag gccgattcac tgttgttgaa
     2761 gacatctgcg tgcttgatgg agagaaaagg cttcagatga tgcagaagtg gccggtaaga
     2821 aaaggaagac cctttgccaa gaagctgaac ccggatgtgc cactgttgac aggacagagg
     2881 gtaatagaca ccttcttccc cgttgcaaag ggtggaacag cagctattcc aggacccttc
     2941 ggttcaggaa agacagttgt ccagcaccag ttggccaagt gggcggatac tgagatagtt
     3001 gtatatgtag gctgtggtga gcgtggaaac gagatgacgg atgttcttat ggagttccct
     3061 gaaatcatcg acccaaaaac cggggagtca ctgatgaaga gaacagttct tattgccaac
     3121 acctcaaaca tgcccgtagc cgcaagggag gcatcaatat atacaggaat aacaattgca
     3181 gaatacttca gggacatggg gtattcagtt gccataatgg ctgactccac atccagatgg
     3241 gcggaggcac ttagagagat gtcaggacgt cttgaggaga tgcctggtga tgagggatat
     3301 ccggcatacc tggcatcaag ggcagcagcc ttctacgaga gagccggaaa ggtcatatgt
     3361 gaaggtgggg acaagagaga aggagccatc accgtaatcg gtgcagtatc gcctccagga
     3421 ggagatattt ccgagccggt atcccaggct acgcttagga tagtaaaggt attctggggt
     3481 cttgaccatg ctctctcata caagaggcac ttcccggcaa tcaactggct aaactcctac
     3541 acactatatc agaccaagat ggaccagtgg atggcaggaa acatagaccc tgagtttgca
     3601 aaaaacaggg ccaaggccat gaaccttcta caggaggagt catcacttca ggaaattgtt
     3661 agacttgtgg gtagggattc actttccgag gaagaccagc ttaagcttga ggccacaaag
     3721 tcactgaggg aggactacct gcagcagaat gcattccatg acattgacac cttctgttca
     3781 ttggagaagc agaacaagat gctaaagctt gtacttcagt tctatgacct tggtatgaag
     3841 gctctggaaa agggagttta ccttaaggac atcgagcata tgccaataag ggaaaacata
     3901 gcaagaagca agtatataga tgaggaagaa ctccacaaga tagacgagat aggcgtggag
     3961 cttaaccagg aaataaatag actcatagag gaaggaggta tcctggatgc gtaaggaata
     4021 taataccgta agggaagtag ttggaccatt gatggttgtt gacggagttg aaggcgtcaa
     4081 gtacgaggag cttgtggaca ttgagctggg tacaggcgag aaaagacgtg gtagagtcct
     4141 tgaggtaaac ggcgacaagg ccatggttca gctttttgaa ggatcctccg gaatcaacct
     4201 tggacagacc agggttaagt ttcttggaaa gccccttgag cttggagtaa gcgaggacat
     4261 gatcggacgt atattcgatg gccttggaag gccaaaggat gatgggccaa agatcatacc
     4321 agagaaaaag gttgatataa acggtgtgcc aataaatccc gtatcaaggg actatccaga
     4381 agagtttatc cagactggga tttcatgtat agacggtctg aacaccctgg taaggggaca
     4441 gaagcttccg attttctccg gatcgggtct acctcacgga gaggttgccg cacagattgc
     4501 aagacaggca caggtactgg gtaccgatga aaactttgcc gttgtgtttg cggcaatggg
     4561 tataaccttt gaggaagccc agttctttat agaggacttt acaaggacag gggccataga
     4621 cagggcggta ctattcgtaa accttgcaga tgaccctgca atcgagagga tcgcaactcc
     4681 aaggatggca cttacctgtg cagagtatct tgcttttgag aaggacatgc acgtacttgt
     4741 tatcctgaca gacttgacaa actacgctga ggctcttcgt gagacatcag ctgccagaaa
     4801 ggaagtgcca ggtcgtaggg gatatccggg atatctttac accgaccttt ccacaatata
     4861 tgaaagagcc ggcaggataa ggggaagaaa gggttcaata acccagattc caatcctgac
     4921 aatgccagag gatgacataa ctcacccaat accagacctt acaggataca tcacagaggg
     4981 acagataata ctatcccgtg ccctctataa aaaaggcatc gagcctccaa taaacgtcgt
     5041 accatcactg tcccgtctta aggacaaggg tataggagat aacaagacaa gggaggacca
     5101 tgcggatacc atgaaccaga tctatgccgc atacacctcc ggtattgagg ccagggagct
     5161 tgcaaccata cttggtgagt cagccctatc agatgccgac aaggcctttg caaagtttgc
     5221 tgaagcc
//
LOCUS       DASRQF010000242         5643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1023751, whole genome shotgun sequence.
ACCESSION   DASRQF010000242 DASRQF010000000
VERSION     DASRQF010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023751"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="VFD79_06405"
     CDS             complement(<1..1226)
                     /locus_tag="VFD79_06405"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HZH93715.1"
                     /translation="MNYREKYQSWIDDPFFDEATKAELMGLTDEDEIEDRFYQDLEFG
                     TAGLRGKLGAGTNRMNIYTVSMATEGLARTIENYGDEAKKKGVAIAYDVRLYSDEFAQ
                     IASRVLAAKGIKVYLFDDIRPTPMLSYAVRKLGTQSGIVVTASHNPQDYNGYKVYWEE
                     GSQILDDVADEILEEIDGIDHYRDIELMDFEEAKEKGLIQIIGKEIDDEYFSELLKMT
                     IHDAIDKDVKIVFTPLNGTGNIPVRRILRERGFNNIIVVPEQAEPDYTFATVGYPNPE
                     DNKAFNYARELGLEEDADVLLATDPDCDRVAIAVKNDKQDYTYLNGNQIGALLVYYIL
                     SERSKKGNLPENPAIVKSIVTGDLGKAIAKKYDVATFETLTGFKNICAKANEWDETGA
                     FSFVFGYEESIGYVYK"
     gene            complement(1316..4447)
                     /locus_tag="VFD79_06410"
     CDS             complement(1316..4447)
                     /locus_tag="VFD79_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZH93716.1"
                     /translation="MSFGKFSTGKKYIVLSIIIGMVIFGIYSMFTLNTQLSPDTSPPA
                     ATVMSVYPGAMALDVSTDVSKPMEEAFAKLDGIENISSSSQDNISIIRLTFDYSTDVD
                     QAAIDIQNTIATIKNKLPESLQDPQVLKFSVSDQPIMTIGLMSDSLSMKDLRSLTEDK
                     LAYELQLVEGVAAVNIFGGYSQEILVNLDEATVRAYGLSMETIVGSLKTANIKAPGGS
                     ILYEDRDLLLRVEESLLSIQELENINIPLGDGNSVPLSVLGEVESGTVDREGSYRLNG
                     EDALAILITKKADYNTVDVIETINDKMVVLEEAYPYVQMTVASDDSQFTNQMVSNMAT
                     SVLIAIAFTMIIILLFINNISRSLVISISMPLVFLSTMGLMKLFHMDLDLVTLSALIL
                     SIGFVVDTAIVVVENISSHVGQGKEITRAAIEGTDEIALPSIAGATTTLIVLIPLMFI
                     TGFVGEMFRPLSATLIFAISSSLMVALVIIPLLTVMFQPLEFRRTEKLVSKLSQPFNK
                     IMDSIQEKYIALFHKTRKRKKRALLVMVILMALSALFLRSNGMEMLPKFDSGVSYVTI
                     EMVPGTTLEKTEEAVGLIEDYLLQLEEVVSFDSRIGYEEGNIQQGDFGIMGSDQAMIT
                     INLTSRKDRKLTIWDLQERLREEIERIPDVNRYVVKEKGGTAVTGASAPIAIKITGED
                     LDVLYHIADETLERVEHVPGTTNVFTSFNNDYRQMSLKLDKERLSELGLTSAAVSTQL
                     YGRMEGIDATSMTLGREDSIDVKVGYSGNSSSDLDYLMDTYLTTPLGVRIPLGEVASI
                     QVENRANMVERENMSYTVNISGFTEGRAFSHVVSEIQDEIDRMVLPMGYSIEFSGEQQ
                     TLTDSVGDMVFLISLAIVFVYLILVPQFKSFLHPITIMAAIPMVVIGIAPALALTGKY
                     MSMPVLLGLILLAGTVVNNSILLVSAINDNRATGMEISSAIEDAIRSRFRPIMMTALS
                     DIVGMMPLALQLALGSERFSPLAITVIGGMLAATLLTIIIIPLIYMTLEGAGARLNRT
                     LGRSSPSLETEPS"
     gene            complement(4444..>5643)
                     /locus_tag="VFD79_06415"
     CDS             complement(4444..>5643)
                     /locus_tag="VFD79_06415"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZH93717.1"
                     /translation="IGLLPIEAGKSDSEIVEQGIVTQLHSVSRVQMIQDLTYLGTLES
                     ETKAMLSPKVTGNIVSINVSEGDIVHQGEVLLQIDSEQLEAKIRTMEQKKNLIYETMS
                     YLDSELEGFHSTNPLASKLQSARLSLDFQVSEVEKLRTLYEAGAISSSQLDQGEHQLK
                     AMRLQVQELEQTMDSSFNKLQQERDVATAQLEELEASLDEMELSLRDSFIKAPFDGQI
                     TQVMVSVGELASPGRTLVSLDNVHQLRVVTQVAESDLQKIRKGMDVEVTLSGLGDPIE
                     GHITYKSGSVNPKTRIGEVRVGVELSEDEVLIGSSATIRIFLDTSRNEIMIPSSAVKT
                     LETGRVVYVYNGNGRVYERKVNIGNSIGDQYQVIEGLAEGDIIAVRNIQSLSDGTLVY
                     TLEEVTQ"
BASE COUNT         1463 a         1387 c         1037 g         1756 t
ORIGIN      
        1 tccttgtaga cataacctat actttcttcg tatccgaaca caaagctgaa ggctcctgtc
       61 tcgtcccact catttgcctt ggcgcagatg tttttaaagc ctgtcaaggt ttcaaatgta
      121 gcaacatcgt atttctttgc aatggcttta cccagatctc cggttactat acttttgact
      181 atggctggat tttctggcaa gttacctttc ttacttcttt cagatagaat gtagtataca
      241 agcaaagctc caatttggtt gccgtttagg taagtgtagt cctgcttgtc attcttgaca
      301 gcaatggcca ccctgtcgca atccgggtca gttgcaagca atacatcagc atcctcttca
      361 aggcccagtt cccttgcata attaaaggcc ttattgtcct cagggtttgg atatcctact
      421 gttgcaaatg tatagtcagg ttcagcctgc tctggtacaa ctataatgtt gttaaagcct
      481 ctttccctta ggattcttct tacgggaata ttaccagttc cattcagggg tgtgaagact
      541 atcttcacgt ctttgtctat agcgtcatgt atggtcatct ttagaagctc tgagaagtac
      601 tcatcgtcta tttccttgcc gattatttgg atcaagccct tttccttggc ttcctcaaaa
      661 tccatcaatt caatgtctct gtaatggtca atcccatcga tttcttccaa tatctcatct
      721 gccacatcat ccagaatctg tgatccttct tcccaatata ccttataccc gttgtagtcc
      781 tggggattat ggctggctgt cacaactatg cctgactgag tgcccagttt tctaacagca
      841 taggaaagca ttggtgtagg tcttatgtcg tcaaacagat atactttgat gccctttgca
      901 gccaggactc tggatgctat ttgggcaaat tcatcagagt ataatcgcac atcatatgca
      961 atcgcaaccc cttttttctt ggcttcatca ccatagttct ctatggtcct tgccagtccc
     1021 tcggttgcca tgcttacagt ataaatgttc attctgttgg ttccagcccc aagcttaccc
     1081 cttaatccag cagttccaaa ctcaagatcc tggtagaatc tatcctcaat ctcatcctcg
     1141 tcggtcaatc ccataagctc agcttttgtt gcctcatcaa aaaatggatc atcaatccat
     1201 gattgatact tttctctata gttcatcttc atccccccat tggtagtatc cttacatatc
     1261 tctataattt ataccctcta ttgattcaaa tacccatcct tttgacaata gaattctaac
     1321 ttggttcagt ctccaaagaa ggtgaactcc ttcccaaggt tctgttgagt ctagctcctg
     1381 ccccctccaa ggtcatataa atcaagggga tgatgataat tgtcaaaagg gtcgctgcaa
     1441 gcattccacc tattacagtt attgccaggg gtgaaaatct ttcagatccc aatgccagct
     1501 gcagtgcgag aggcatcata ccaactatgt cagacagggc agtcatcata ataggtctga
     1561 atcttgacct tatggcatcc tctattgcac ttgaaatttc cataccagta gctctattgt
     1621 cgtttattgc tgacaccagc agaatggagt tgtttactac tgttcctgcc agtaatatca
     1681 atcccaatag aactggcatg gacatgtatt ttcctgtcaa ggccaatgct ggggctatac
     1741 caatgaccac catgggaatt gctgccataa tggtgatggg atgcaggaag gacttgaact
     1801 gtggtaccag tataaggtat acaaacacta tggccaggga aatcaaaaac accatgtccc
     1861 caacagaatc cgtcagagtc tgttgctctc cgctaaattc tatgctatag cccattggca
     1921 gaaccatcct gtctatctca tcctgtattt cagaaaccac atgactgaat gcacggccct
     1981 cagtgaatcc gcttatgttc acagtatatg acatgttttc tctttccacc atattggccc
     2041 tgttttcaac ctggattgag gctacttcac caagtggaat ccttactccc aatggtgttg
     2101 tgaggtaggt atccataaga taatcaaggt cagatgagga attcccactg tatcccacct
     2161 ttacatctat ggagtcctca cggcccaggg tcattgatgt ggcatctatg ccctccatcc
     2221 ttccatagag ctgggttgat accgctgcac ttgtaagacc tagttcagag agtctttcct
     2281 tatcaagctt aagagacatc tgcctgtaat cattattgaa gcttgtgaat acattggtgg
     2341 tccctggtac atgttcaacc ctttcaagtg tttcatctgc aatatgatag agcacgtcca
     2401 agtcttcacc agtaatcttg attgctatgg gggcagaagc tcctgttacg gctgttccac
     2461 ccttttcctt tacaacatat ctgttcacat caggtattct ttcaatttct tctctcagtc
     2521 tctcctgaag gtcccatatg gttaactttc tatccttcct tgatgtgaga tttatggtta
     2581 tcattgcctg gtcacttccc atgattccaa agtcaccctg ctggatgtta ccttcctcgt
     2641 agcctattct agaatcaaat gagaccactt cctcaagctg aaggagatag tcctcgatga
     2701 gtcccactgc ttcctcagtt ttctccaggg ttgttcctgg aaccatctca atggttacat
     2761 aggaaactcc gctatcaaac tttggcaaca tctccattcc attggagcgt aaaaacaatg
     2821 cactcagtgc catcagtatt accatcacca gcaaagccct ctttttccgc tttcttgttt
     2881 tatgaaaaag agcaatatat ttttcctgta ttgaatccat gattttatta aatggttgtg
     2941 aaagctttga aaccagcttc tcagtccttc taaactccag aggttggaac atcacagtca
     3001 gcaaaggaat aatcactaat gcaaccatta acgacgaaga aatcgcgaat atcaatgtgg
     3061 ccgatagggg tctgaacatc tctccgacaa aacctgtaat gaacatcaag ggtatcagta
     3121 ctatcaaggt tgttgtagca cctgctatac ttggtagtgc tatctcgtct gttccttcta
     3181 ttgcagctct tgttatttcc tttccctgcc caacgtgact gcttatgttt tccacaacca
     3241 ctatggctgt gtctaccaca aaccctatgc tcaatatcaa ggcagacagt gtaaccaggt
     3301 caaggtccat atggaatagc ttcatcaacc ccatggtgct taagaatacc aggggcatgg
     3361 atattgaaat taccagggat ctcgaaatgt tgtttatgaa caatagaatt attatcatgg
     3421 taaatgcaat ggctatcaga acacttgtgg ccatgtttga aaccatttga tttgtgaact
     3481 gtgaatcatc agaggcaacg gtcatctgca cataaggata agcctcctcc agcaccacca
     3541 tcttatcatt aatggtttct atcacatcca ctgtattgta atctgctttc ttggtaataa
     3601 gaatagccaa ggcgtcctca ccatttagcc gatatgaacc ttccctgtca acagttcctg
     3661 attcaacctc ccccagaaca gaaaggggaa ctgaattgcc atccccaagt ggtatgttta
     3721 tattctccaa ctcctggatt gatagaaggc tttcctcaac ccttaggagc aagtccctat
     3781 cctcataaag tatgcttcct cctggtgcct ttatattggc ggtttttagc gaaccgacga
     3841 ttgtttccat ggacaatcca taggccctga cagtagcttc atccagatta accaatattt
     3901 cctggctata gcctccaaaa atattcacag ctgctacacc ctcaaccaac tgaagctcgt
     3961 atgcaagctt gtcctctgta aggcttctca agtccttcat ggaaagagaa tcactcatta
     4021 aacctattgt cataatgggc tgatctgaaa cgctaaattt cagtacctga ggatcctgaa
     4081 gactttcagg taatttattc ttgatggtgg caatggtatt ctgaatatca attgctgcct
     4141 ggtcaacatc agtggaataa tcaaaggtta atcggattat cgaaatattg tcctgacttg
     4201 atgaactgat gttttcaatt ccatcaagct tcgcaaatgc ttcctccatg ggtttagata
     4261 catctgttga cacatccaga gccattgccc caggatatac gctcataact gtagcagctg
     4321 gaggactggt atccggtgat agctgggtgt tcaatgtgaa catgctgtag atcccgaaaa
     4381 tcaccatacc aattattatg gaaagtacaa tatacttttt acctgtggag aattttccaa
     4441 aactcattgt gtgacctcct ccaaagtgta tactagtgtg ccatcactta aggattggat
     4501 gtttctcacc gctatgatgt cgccttcggc aagtccttca ataacctgat attgatcacc
     4561 aatactgttt cctatattta cctttctttc atagactctc ccattaccat tgtatacata
     4621 gaccaccctt ccagtttcaa gggttttaac agcagatgag ggtatcataa tctcattcct
     4681 gctggtatcg agaaaaattc taatggttgc gcttgaacct attaggacct catcctctga
     4741 aagctcaact cccactctaa cttcaccaat tcgggtctta gggtttacac ttccactctt
     4801 ataggtaata tgaccctcaa tggggtctcc aagtcctgaa agggttacct caacatccat
     4861 tccttttctg attttttgaa gatccgattc cgccacctgc gtcactaccc taagctggtg
     4921 tacattatcc agacttacaa gagtccttcc aggagacgcc agctcgccaa ctgacaccat
     4981 gacctgagta atttgaccat caaagggagc ctttatgaag gaatctctca gcgacagttc
     5041 catctcatcc agggaagcct caagctcttc cagctgggct gtagccacat ccctttcttg
     5101 ttgaagctta ttgaagcttg agtccatggt ctgctccaac tcctgaacct gcagtctcat
     5161 ggccttaagt tgatgttcac cttgatcaag ctgtgaactt gatattgccc ctgcttcgta
     5221 taaggtcctt aacttttcta cctctgatac ttgaaagtca agtgatagtc ttgcagactg
     5281 aagctttgag gccaatggat tagtcgaatg gaaaccttcc aactcagaat ccagatatga
     5341 cattgtttca tagattagat tctttttttg ctccatggtc ctaatcttgg cttccaattg
     5401 ttcactgtca atctgaagta gaacttctcc ctggtgtaca atatctcctt cagatacgtt
     5461 tatactgact atattcccag tgacttttgg gctaagcatt gcctttgttt cactttcaag
     5521 ggttccaagg tatgtcagat cctggatcat ttgcaccctt gaaacagaat gaagctgtgt
     5581 cacaataccc tgctccacaa tctcagaatc ggacttgcct gcctcaattg gcagtaatcc
     5641 aat
//
LOCUS       DASRQF010000243        13178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_321036, whole genome shotgun sequence.
ACCESSION   DASRQF010000243 DASRQF010000000
VERSION     DASRQF010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 13178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13178
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321036"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VFD79_06420"
     CDS             complement(<1..410)
                     /locus_tag="VFD79_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93718.1"
                     /translation="MSPSEKVTIQTVRNRREFNQFVKFPYVLYEDDKAWVPPLVGDFK
                     KYVKGEGNYLNQSGPNEKIIAIRDGEVCGRLLVGINEHLNNHKNLKEGYISLFECEND
                     QETAGLMLEFAEAWLVERGMEFVKGPLSLPGGDD"
     gene            complement(431..1423)
                     /locus_tag="VFD79_06425"
     CDS             complement(431..1423)
                     /locus_tag="VFD79_06425"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HZH93719.1"
                     /translation="MKYLTNLSNNPNYNLAFEEYCFKNLPKDEDFVYLWINGPAIIVG
                     KNQNTLEEINSEYVKENDIKIVRRITGGGAVYHDLGNLNFSIYTNSKGQERIDFGQIN
                     IPILKALEKFGIKAELSGRNDLTLDGKKISGIAQSVYRKRILNHGTILYDTDMSVLSK
                     ALNVKADKIESKGVKSVRSRVTNIRPYMQEDASILEFKDMLLKNIFQYHNEEMEEYVL
                     SQQELDGIQKLYEEKYSTWEWNYGESPDFGWKNYKRFPSGSVEVRLEIKNGVINDAKI
                     FGDFFGTKDVEDLVETLKGVRYDKDNVKELLANIKISDYFSNMADSEFIELLFD"
     gene            complement(1433..3061)
                     /locus_tag="VFD79_06430"
     CDS             complement(1433..3061)
                     /locus_tag="VFD79_06430"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZH93720.1"
                     /translation="MQLSFFGAAQMVTGSNYLLSTKNHNIVIDCGMFQGNQETEKLNF
                     KEFPFDPASIDIMILTHAHIDHSGRIPKLVKEGFRGRIISTKATYDLCGVMLLDSAKI
                     QEYDAEWENKKRKRRGEPSIEPLYTIEDAEHSLKYFEPHLYEQKIDINGEISIRFKDA
                     GHILGSSILELWITEDEKTTKLVFSGDLGMPDRPIIRDPQYIDEADYLVIESTYGDKD
                     HPPMKESTEKLIEIINKTAERGGTVLIPSFAVGRTQELIYELNRYYEYNDIKPHLEVP
                     IYIDSPMAINATEVFQNNAGTFDQETTDLILKGDNPFTFNNLNYIRSVEESIALNESD
                     IPKVIISASGMATAGRIRHHLKHNLWNPKNSLVFVGYQAEGSLGRILLDGAKKVKLLG
                     EEIHVGLEIYDLEGFSAHADRTFMLNWIEKIEKKPKKVFIVHGERETAKAFSNQIEER
                     FGLKTIIPKMGNSYRVAIDAIEVEKGMTIEPEILREDIAKELQVVYEQFNSLMSRSNG
                     LFEEKLLENDYDLLKNRLLDLQSQLMDLNILIGK"
     gene            complement(3191..4231)
                     /locus_tag="VFD79_06435"
     CDS             complement(3191..4231)
                     /locus_tag="VFD79_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HZH93721.1"
                     /translation="MKNKIIAMLVMVILVGVVATGCGGSQPTLNVYNWGDYIDPEVLD
                     LFEEEFGVRVVYDTYATNEDLYVKMKQGGSSYDVVIPSDYMIERMIREDLLMEINMDN
                     ISMLDKVDDRFLNLDYDPENAYSVPYMWGTVGIIYNTTMVDRPLTSWADLWDEEFEGE
                     IIMLNSQRDTLAVALKKLGYSMNTRDIGELEEARDELIEQKPIVYAYLGDEVKDVIVG
                     GEAAIAVVWSGDAVAMMDENPDLEYVIPSEGTNLWFDAMVIPANSQSPEVAEQFIDFM
                     TRPEIAAMNADYIGYSSPVPEAVDMLDEEIQNSPVAYPSDDILENTEVFKDPQDIISE
                     YDRIWTEITSAQ"
     gene            complement(4236..5027)
                     /locus_tag="VFD79_06440"
     CDS             complement(4236..5027)
                     /locus_tag="VFD79_06440"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZH93722.1"
                     /translation="MVNKNLKRLYTFLIFAFLYAPIIILIIYSFNDSRFRGDWSGFTL
                     RWYVELFKDREVIRVLYYTFAIAITSSIISTVLGTVAAIGIHGMRGFTKKSILNLNYL
                     PVLNPDIVTAVALMALFRMARLQFGFITLLLAHITFSVPYVVLSILPKLKQMNVHLAE
                     AAMDLGATPMYALRKVVIPEIMPGIITGGLLAFTLSIDDFVISFFTTGHGISNLSIYI
                     YSMARKGINPTINALSTLMFISLVALLLIINNRSEKSKKIIQERR"
     gene            complement(5021..5860)
                     /locus_tag="VFD79_06445"
     CDS             complement(5021..5860)
                     /locus_tag="VFD79_06445"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZH93723.1"
                     /translation="MKLNRIGYPYLIWLGIFILIPLLLVTYFAFTTGDSQNFSTFTFS
                     MENFEKFMSPIYLGVLYRSVRLAIISTVICLVLGYPIALIISKEKPKIRNILVLMLVI
                     PMWMNFLLRTYAWLTLLGKNGILTKALSFIGLEGVSLLYNDTAVLLGMVYNFLPFMVL
                     PIFSVLTKMDNNLVEAAEDLGANRSEVFWKVIFPLSLPGVITGITMVFIPAVSTFVIS
                     GLLGGNKSTLIGNLIEQQFRYTGDWHFGSTMSIILMVFILASMALTSKFDKEKEGGGG
                     GLW"
     gene            complement(5850..6908)
                     /gene="potA"
                     /locus_tag="VFD79_06450"
     CDS             complement(5850..6908)
                     /gene="potA"
                     /locus_tag="VFD79_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:1902047 - polyamine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein"
                     /protein_id="HZH93724.1"
                     /translation="MMTKTHAIDLVQIYKQFGDTPVLEDINLYIRQNEFLTLLGPSGC
                     GKTTTLRIIGGFETPTSGRVMFEGEDITELPSFKRQINTVFQKYALFPHLNVFDNIAF
                     GLKIKKMSKETIRTKVEEMLKLVNLQGFEDRDIDSLSGGQQQRIAIARALVNEPKVLL
                     LDEPLGALDLKLRKDMQLELKRIQKRVGITFIYVTHDQEEALTMSDTIVVMDRGRIQQ
                     IGTPTDIYNEPINRFVAKFIGESNIVDGIMNEDFKVTFSGRAFECVDKGFGLDQPVDV
                     VIRPEDLQIVAYDEGYISGQVESVIFKGVHYEMEIRDDDYTWTVHSTIMKNVGEKVGM
                     IIVPDNIHIMRKGEADEA"
     gene            complement(6916..7455)
                     /locus_tag="VFD79_06455"
     CDS             complement(6916..7455)
                     /locus_tag="VFD79_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HZH93725.1"
                     /translation="MNFGEKIKAIRVKKNLTQEELADRCELTKGFISQVERDLTSPSI
                     ATLVDILEGLGTNLKDFFNEEEEEKIVFRKEDVFETVNEDLKYVLHWIIPNAQKNAME
                     PIMVTLSQGGRTKEDSPHEGEEFGYVLSGTIYLHLGNEKYKVRKGESFYYRTNSDHYI
                     ENAGKSEASLIWVSTPPNF"
     gene            complement(7650..8816)
                     /gene="thiI"
                     /locus_tag="VFD79_06460"
     CDS             complement(7650..8816)
                     /gene="thiI"
                     /locus_tag="VFD79_06460"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HZH93726.1"
                     /translation="MERVISVSLGEVVLKGLNRKYFEDRLIKNMKRVLRDFGEYRIYK
                     EQGKIYLEGLDNHQEDAIRRLKKVFGLVYISPCIRTSRDMEDIREAGLALVQEKVPGD
                     KTVSFKVETSRSDKRYPLKSPEISRDIGGYVLKNTDNLKVDVHSPDFMLYVDVKEYAY
                     VYIDRTRAYGGLPIGTNGKGLLLLSGGIDSPVAGFMMAKRGVEVDAVHFHSYPFTSQR
                     AEEKVMDLAKIVSMYTGPMKIYSINLLNIQKEINKNCPEDEMTILSRRFMMRIAEKIS
                     DKSGYQALITGENLGQVASQTIFSMDVINRSVQMPILRPLVGMDKVDIIEISKDIETY
                     ETSIKPFEDCCSVFLPKHPVTRPKIQDIEESEKALDIESLVQEAIDGMKITNIE"
     gene            complement(8821..9975)
                     /locus_tag="VFD79_06465"
     CDS             complement(8821..9975)
                     /locus_tag="VFD79_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HZH93727.1"
                     /translation="MIYLDNCSTTRPRDLVVEKMTDALRISFGNPSSLHRMGMDAEKI
                     MKDSRSVIASYLGVKNEEIYFTSGGTEGNNIAIQGALDARKSRGNHIITSMIEHPAVM
                     NTIKEYEKKGFRVSWIPNGKDGRIDLKRLEEELCDETILVSLIHVNNEIGTVQDIGAV
                     GDLLSKSPSKPHFHLDGVQSFGKIQFSLIGNGVDSYAFSGHKIHGPKGVGGLYINRKA
                     RVNPVIFGGNQEMGMRSGTENIPGIAGFGEAVRILKDQGLIESEKVGALKDLMTKSLM
                     EQIEDVIINSPAGDLSSPYILNLSFGWTRGEVLLHYLEEEEIFISTTSACSSKGTDKS
                     HVLKSIGLDDNHIEGTVRICFSYEISEEDVYRTVKALKDSVEMVRSVMRR"
     gene            complement(9987..10580)
                     /locus_tag="VFD79_06470"
     CDS             complement(9987..10580)
                     /locus_tag="VFD79_06470"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HZH93728.1"
                     /translation="MDKVIKALEKNGFTASIFKTAQDAKEKLLNEIAIGESVGIGGSM
                     TILDMGVYEELMERGNDTYWHWKAEDKKAALEYATTSDVYLTSSNALTEDGKLVNKDG
                     TGNRVASMIFGHKKVFVIVGKNKVCKDVDAAFKRIETVAAPLNAKRLNLTTPCVYTGV
                     CSDCDSKDRICKAETILSKNPNGTSIHVYIIDEDMGY"
     gene            10920..11906
                     /locus_tag="VFD79_06475"
     CDS             10920..11906
                     /locus_tag="VFD79_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HZH93729.1"
                     /translation="MTKKLAIIFTGGTISMKIDPEIHAAIPALNADEIVSSLKNIEDI
                     AEFEVVNYANLPSPHITPAIMMDIYHLVTDRLADPTIDGVIVTHGTDTMEETAYMLDL
                     LIDSDKPVVIVGAMRNFSELGYDGASNLSAAICTAVSDLSKGKGVLVVMNNEVNAASQ
                     VTKTNTLSLDTFKSPEFGPLGIVDNDEVLYYRNIVSRQVIETEKLEEKVALIKTGAGM
                     ESDIMDFCIDRGYKGIVIEAMGRGNLPPAMVPGVKRAIQNNVPVVMVSRCNTGRVLYT
                     YGYEGGGRQLRDMGVILGGNLPGQKARMKLMLILGKTSDYNEVRELFELNLY"
     gene            complement(11954..13147)
                     /gene="thrC"
                     /locus_tag="VFD79_06480"
     CDS             complement(11954..13147)
                     /gene="thrC"
                     /locus_tag="VFD79_06480"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZH93730.1"
                     /translation="MEKLICTNCSETYDLETETGRCTVCGEPLETEPITNGVIADEFP
                     MEKGIFNRYASFFPFLDLDLALSLGEGSTPLVHIREGTNGVKGSKIYIKNEGMNPTWS
                     FKDRGTLTGLLHAKAMGYNSVGTVSTGNMAASVAAYAARAGLHATILVKEEMADEKIG
                     PIGIYGPRLIKVKGNYRKLYTESIRLGRENGIYFINSDAPYRVEGYKTSSFEIFQQLG
                     MKVPDYVIVPTSAGGNFRGIEKGFRELYLSGITDRMPIFIASQARGCNPIVRAFDSGS
                     EEVQQLEEFGTIAHAIENPYPPSGNQVLRLIRKNGGMAVDVSEDEIINHQKILANAGI
                     FVQPASATSLAAANRLMEEGKIESDAEVVCIATASGLKFTESLKYHTIKVENCDIDDL
                     ESRLR"
BASE COUNT         3422 a         3151 c         2369 g         4236 t
ORIGIN      
        1 ttgtcatctc cacctggtag tgacaatggc ccctttacaa actccatccc cctttccacc
       61 agccatgcct ctgcaaattc aagcattaag cctgcagttt cctggtcatt ctcacactca
      121 aaaagtgaaa tgtacccttc cttgagattc ttgtgattat tcaagtgttc attgattccc
      181 accaatagtc tgccacatac ttccccgtcc cttatagcta tgattttttc gttggggcca
      241 gactggttta gatagttacc ctcaccctta acatactttt tgaagtcccc aaccaatggt
      301 ggaacccaag ccttgtcatc ttcataaagg acatatggaa attttacgaa ctgattaaac
      361 tctcttctat ttctaacagt ctgtatagta accttttcac ttgggctcac ttaattcatc
      421 tcccatggat ttaatcaaac aaaagctcta taaactcaga atcagccata ttgctgaaat
      481 aatctgatat tttaatgttt gccaacagtt ctttgacatt gtctttgtca tatcttacac
      541 ctttaagtgt ctccaccagg tcctcaacat cctttgtccc aaagaaatct ccaaatattt
      601 ttgcatcatt tatcacgcca ttctttattt caagtcttac ctcaaccgaa ccagatggga
      661 atcttttgta attcttccat ccgaaatccg gggattcacc ataattccat tcccaggtag
      721 agtacttctc ctcgtaaagt ttttgtatgc catcaagctc ctgttggctt aatacatact
      781 cttccatttc ctcattgtgg tactggaaaa tgttctttag cagcatgtcc ttgaattcaa
      841 gaattgatgc atcttcctgc atgtatggtc tgatgtttgt aacccttgac ctaactgatt
      901 ttactccctt ggactcaatc ttatccgcct tcacattaag tgccttggaa agaacactca
      961 tgtctgtatc gtataggatt gttccatggt tcagtattct tttcctatat accgattggg
     1021 caattccgga tattttcttt ccgtcaaggg tcaggtcgtt tctaccggac aactcagcct
     1081 tgatcccaaa tttttccaat gccttcagta ttggtatgtt gatctgacca aagtctattc
     1141 tttcctggcc cttggaattg gtatatattg aaaaattaag gttccccaga tcgtggtata
     1201 ctgccccacc accggttatt ctccttacaa tctttatatc attttcctta acgtattcgc
     1261 tgttgatttc ttccaatgtg ttctggtttt tgccaacaat tattgcagga ccgtttatcc
     1321 aaagatatac gaaatcttca tccttgggca agtttttgaa gcaatattcc tcaaatgcca
     1381 ggttgtagtt tgggttgttt gatagatttg tcaaatactt catttcttcc tcctattttc
     1441 caattaaaat atttaagtcc attagttgac tttgcaaatc aagcaatctg ttcttaagaa
     1501 ggtcataatc attttccaaa agcttttctt caaacagtcc atttgaccta ctcataaggg
     1561 agttgaactg ttcataaacc acttgcagtt ccttggcaat gtcctccctc aggatttcag
     1621 gttcgattgt catccccttt tcgacttcga tggcatcgat tgccacccta tatgaattgc
     1681 ccatctttgg tatgattgtc ttcagtccaa acctttcctc gatctgattg gaaaaagcct
     1741 ttgcagtttc cctttctccg tggactatga aaactttctt tggctttttc tcaatctttt
     1801 caatccaatt aagcatgaat gtcctgtctg catgggctga aaagccttcc aggtcatata
     1861 tttccagtcc aacatgaatc tcttcaccca gcagcttaac cttcttggcc ccatcaagga
     1921 gtatccttcc caaagaccct tcagcctgat atcctacaaa aaccaggcta ttcttgggat
     1981 tccacaggtt atgcttcagg tggtgcctga ttctgcctgc agtagccatg ccactggctg
     2041 agattatcac cttggggata tctgattcat tcaatgcaat ggattcctca accgacctaa
     2101 tgtagttcag gttgttgaag gtaaatgggt tgtccccctt tagtataagg tccgtggttt
     2161 cctgatcaaa ggttcccgcg ttgttttgaa atacctctgt tgcatttatt gccattgggc
     2221 tgtcaatata tattggcact tccagatgag gcttgatgtc attgtactcg taatacctgt
     2281 ttagctcata tatgagctcc tgagtccttc caacggcaaa ggatggtatc aatacggtcc
     2341 cgccacgttc agctgtcttg ttgattatct cgattagttt ttcagtgctt tccttcatgg
     2401 gaggatggtc cttgtctcca tatgttgatt cgataacaag ataatctgcc tcgtcaatgt
     2461 attggggatc tctgattatt ggcctatcag gcatacccag gtctcctgaa aaaacaagct
     2521 ttgttgtctt ctcatcctct gtgatccaca gttcaagaat tgatgatccc aaaatatgac
     2581 cagcatcctt gaatctgatg gaaatttctc cattaatgtc tatcttctgt tcgtataaat
     2641 gtggctcaaa gtattttaaa ctatgctcag catcctcaat tgtataaaga ggttctattg
     2701 atggctcccc tcttctcttt ctttttttgt tttcccactc tgcgtcatat tcctgaatct
     2761 tggcactatc cagaagcatt accccacaca ggtcataggt ggcctttgtt gatatgatcc
     2821 tgcccctaaa tccttccttg accaacttgg gtatcctccc gctgtggtct atgtgagcat
     2881 gggtcagtat cattatgtca attgatgcag gatcgaatgg aaattcctta aagttcaatt
     2941 tctcagtttc ctgattacct tgaaacattc cgcaatctat gacaatatta tgatttttgg
     3001 tgctcaatag ataatttgaa ccggttacca tctgggctgc tccaaaaaag ctcaattgca
     3061 tagccatacc tccttcagtt agtttgttgt cgtccttctc accatgtttt tacacactta
     3121 tggctacatt ataccattaa atcaaaaaag atggtaactt cctcttacaa gaagctacca
     3181 tcagtttcat ctactgtgca ctggttattt cagtccatat tctatcgtat tcacttatga
     3241 tgtcttgagg gtccttgaag acctctgtat tttcaagaat atcatcagat ggatatgcta
     3301 ccgggctgtt ctgaatctct tcgtccagca tgtccactgc ctctggcact ggtgatgaat
     3361 agcctatata atcagcattc atggcagcaa tttcaggacg tgtcatgaag tcgatgaatt
     3421 gttctgccac ttccggactc tgtgaattcg ctggaattac catggcatca aaccatagat
     3481 ttgttccctc tgatgggata acatactcca aatccggatt ctcatccatc attgccacag
     3541 cgtcacccga ccaaacaact gcaatggcag cctctccacc aacaattaca tccttgacct
     3601 catctccaag ataggcatat actattggct tttgctctat aagttcatcc cttgcttcct
     3661 ccagctcacc tatgtctctg gtattcatgg aataacccaa tttcttcaga gccactgcaa
     3721 gggtatccct ctggctgttg agcatgatga tttcgccttc aaattcctcg tcccacaggt
     3781 ctgcccagct ggtaaggggt ctgtccacca tggttgtgtt gtaaattatt cccactgttc
     3841 cccacatata gggtactgaa taggcatttt caggatcgta gtcaagattt agaaacctgt
     3901 catccacctt gtccagcatg gaaatattgt ccatattgat ttccataaga agatcttccc
     3961 ttatcatcct ctcaatcatg taatcagaag ggattactac atcatagctg cttcctccct
     4021 gcttcatctt tacataaagg tcttcatttg tggcatatgt gtcataaaca acccttactc
     4081 caaattcctc ctcaaagagg tccagaacct cagggtcgat gtagtctccc caattgtata
     4141 cattcaatgt cggttggctg ccaccacatc cagttgcaac aacaccaacc aggataacca
     4201 tgacaagcat tgctataatt ttgtttttca tatttctacc ttctttcctg aattattttt
     4261 ttggatttct cgcttctgtt atttattatc aagagcaatg caaccaatga aatgaacatc
     4321 agtgttgaca gtgcgtttat tgttggatta attcccttcc tggccattga gtaaatgtat
     4381 atgctcaaat tggaaatccc atgtcctgtt gtgaaaaagc ttattacaaa gtcatctatg
     4441 gacaatgtaa atgccaatag tccacctgtg ataataccag gcattatttc tggaatcact
     4501 acctttctaa gtgcatacat gggggtcgcc cccaaatcca tcgcagcttc tgcaaggtgt
     4561 acattcatct gcttaagttt cggtaatatt gaaaggacca catacggtac ggaaaatgtt
     4621 atgtgtgcaa ggagcagggt gatgaatccg aattgcaacc tagccattct gaacaatgcc
     4681 attaaggcaa ccgctgttac tatgtccgga ttcagtaccg gcaggtagtt taggtttagg
     4741 atggacttct tggtaaagcc tctcattccg tgaattccta ttgcagcaac tgttccaagg
     4801 actgttgata ttattgagga agttatagca attgcaaagg tatagtatag aacccttata
     4861 acttccctat ccttgaaaag ctcaacatac caccttaagg taaagccact ccagtcccct
     4921 ctaaatctcg aatcattgaa ggaatatatt ataaggatga taatgggtgc atacaaaaat
     4981 gcgaatatca agaatgtata cagccttttc agattcttgt ttaccatagt cccccacctc
     5041 ctccttcctt ctccttgtca aatttggagg tcagtgccat ggaagcaagt atgaagacca
     5101 taagaattat cgacatggtg gatccaaaat gccagtcacc agtatacctg aactgctgct
     5161 caataaggtt acctatgagg gtggacttgt tacctcccag taggccagaa attacaaagg
     5221 tgcttacagc cgggatgaac accatggtta ttcccgttat gactccaggt aaactcaagg
     5281 ggaaaataac cttccagaat acttccgacc tgtttgctcc caggtcttca gcagcctcaa
     5341 ccaggttatt gtccatcttg gtcagcactg aaaatattgg caggaccata aagggcaaaa
     5401 agttatatac cattccaagg agtacagctg tatcattgta gagtagactg accccttcaa
     5461 gtcctataaa ggacaaggcc ttagtgagta tcccattctt gcccagcagg gtcaaccagg
     5521 cgtaagttct caacagaaag ttcatccaca tgggtattac aagcataaga accagtatgt
     5581 tcctgatttt tggcttttcc ttactgatta tcaatgctat gggataaccc aaaaccaagc
     5641 atatcactgt ggatatgatg gccagcctca ctgatctgta tagtacaccc agataaattg
     5701 gagacatgaa cttttcaaaa ttctccatgc tgaaggtgaa tgttgagaaa ttctggctgt
     5761 cacctgtggt aaatgcaaaa tacgtgacca gcaaaagagg tattaggatg aatattccca
     5821 accatatcaa atatggatac cctatcctgt taagcttcat cagcctcccc ctttcgcatg
     5881 atgtgtatgt tatcaggaac tataatcatt cctacctttt ctccaacatt cttcattatg
     5941 gtggagtgaa ctgtccatgt atagtcatcg tccctaattt ccatttcata gtggacaccc
     6001 ttgaagatca cgctctccac ctggccggaa atatatcctt cgtcataggc tacaatctgg
     6061 aggtcctcag gtctgatcac cacatcaaca ggctggtcaa ggccaaatcc cttgtcaacg
     6121 cattcaaagg ctctcccgga aaaagtcacc ttgaaatcct cattcatgat tccatcgact
     6181 atgttgcttt caccgataaa cttggctaca aacctgttga tgggctcatt atagatatcg
     6241 gttggtgttc caatctgttg aattctaccc ctgtccatta ccactatggt atctgacatt
     6301 gtcagtgctt cctcctggtc gtgggttacg tagatgaagg ttattccaac tcgtttttga
     6361 attctcttaa gctccagctg catgtctttt ctaagcttca ggtcaagtgc ccctaacggc
     6421 tcatcaagga gcaaaacctt aggttcgttg accaaggccc ttgcaattgc aatcctttgc
     6481 tgctggcctc cacttaagga gtcgatatcc ctatcctcaa agccttgaag gtttaccaac
     6541 ttaagcattt cttcaacctt ggtccttatg gtttccttac tcattttctt tattttcagg
     6601 ccaaatgcaa tgttatcaaa tacattcaaa tggggaaaga gtgcatactt ctgaaatact
     6661 gtgttaatct gccttttgaa tgagggtagc tcagttatgt cctctccttc aaacatcact
     6721 ctgccacttg ttggggtttc aaatccacca attattctta gggtcgttgt ctttccgcat
     6781 ccactgggac ccagcagggt caggaactcg ttctgcctta tatagagatt gatatcctcc
     6841 aaaactggag tgtctccaaa ttgtttgtaa atctgtacca aatctattgc gtgcgttttt
     6901 gtcatcatac ccctcctaaa agtttggtgg tgtactcacc caaattaagg atgcctcact
     6961 tttgccggca ttttctatat agtgatcact gtttgtcctg tagtagaagc tttcaccttt
     7021 cctcaccttg tatttttcat tgcctaggtg gaggtaaatg gttccggaaa gcacatatcc
     7081 aaattcttca ccctcatgag gggaatcttc ttttgttcta cctccctggg aaagggttac
     7141 cataattggt tccattgcat tcttttgcgc atttggtatt atccaatgaa gaacgtattt
     7201 caaatcttcg tttacggttt caaaaacgtc ttcctttcga aatacgattt tttcttcctc
     7261 ttcctcgttg aaaaaatcct taaggttggt ccccagtcct tccaaaatat caacaagagt
     7321 ggcaatggat ggtgatgtaa ggtctctttc aacctgcgat ataaaaccct ttgtaagttc
     7381 gcacctgtca gcaagctcct cctgggtaag gtttttcttg actcttatag ccttgatttt
     7441 ttctccgaaa ttcatagcgc cctccgtttc attaaacaaa tagtttatgt ttactaaaca
     7501 gctaccacaa tattatagat tagagatatt atgctgtcaa cccatattag tattaaattt
     7561 ctgatgtttt catgaattca atttgtgaaa atattgactg tgttaaacat agagtaaata
     7621 aatagaaaac aaaaaggacc ttgcggtcct tattcgatgt tcgttatttt cattccgtct
     7681 attgcttcct ggacaagact ttcaatatcc aaagcctttt ccgattcttc aatatcctgg
     7741 atttttggcc ttgttaccgg atgttttggc aaaaatacgc tacagcaatc ctcgaagggc
     7801 ttaatcgatg tctcataggt ttcaatatcc tttgatatct caattatatc aaccttatcc
     7861 atcccaacaa ggggtcttag tattggcatc tggacagaac gattgattac atccatgcta
     7921 aaaatagtct ggctggcaac ctgtcccagg ttctcaccgg ttatcaatgc ctggtatcca
     7981 cttttatcac tgattttctc ggcaattctc atcatgaacc ttcttgaaag tatggtcatt
     8041 tcatcctcag gacaattctt attgatctcc ttctggatgt tcaagaggtt aatactatat
     8101 atcttcatgg ggccagtata cattgaaaca atcttggcca aatccattac cttctcttct
     8161 gccctttggg aggtgaaggg atatgagtgg aaatgcaccg catccacctc cacacctctc
     8221 ttagccatca tgaagcctgc cacagggctg tctatgcccc ctgacagcag tagtagaccc
     8281 ttgccattgg ttcctattgg aaggccccca taggcccttg tcctgtctat gtagacatag
     8341 gcgtattcct taacatcaac atagagcatg aagtctgggc tgtggacatc caccttcaga
     8401 ttgtctgtat tctttaggac ataacctcct atatcccttg atatttcagg ggacttcaaa
     8461 ggatacctct tatctgacct gctggtttcg accttgaagg atacagtttt atcaccaggg
     8521 accttttcct gaacaagtgc caggcccgcc tccctgatat cttccatatc cctgcttgtc
     8581 ctgatgcagg gactgatata aaccaggccg aaaaccttct tcagtcttct gattgcatcc
     8641 tcttggtggt tatcaaggcc ctccaggtat atcttgccct gttccttgta tattctatac
     8701 tctccaaagt ccctgagaac ccttttcata ttcttgatca atctgtcctc aaagtacttt
     8761 ctattcaatc ccttaaggac gacctccccg agacttacgc ttattaccct ttccaatgca
     8821 tcatctcctc attacacttc ttaccatttc aacagaatcc ttcaatgcct tgactgtcct
     8881 gtagacatct tcctcactta tctcatatga aaaacatatt ctcactgtgc cctcaatgtg
     8941 attatcatct agtccaatgg attttaacac atgactttta tcggtaccct tggaggaaca
     9001 ggctgaagtg gttgatatga aaatctcttc ttcctccaga tagtgaagca acacctctcc
     9061 ccttgtccat ccaaaagaca gattcagaat gtaaggtgat gacaggtctc cagctgggct
     9121 gtttattatt acatcttcta tctgttccat gagggatttg gtcatcaaat ccttcaaagc
     9181 acctactttt tccgattcga tcaatccctg gtccttaaga atccttacgg cctccccaaa
     9241 tccagctata cccggtatat tctctgtgcc agacctcatt cccatctcct gattcccgcc
     9301 aaagatgact ggattgactc tggccttcct gttgatgtaa aggccaccta ctccctttgg
     9361 accatgaatc ttatgtccac tgaaggcata gctatcaaca ccatttccaa tcaatgaaaa
     9421 ttggatttta ccaaaggact gcacaccatc cagatgaaaa tgcggcttgg atgggctctt
     9481 tgacaggagg tcgccaactg cccctatatc ctggacagta ccaatctcat tgttgacatg
     9541 tatcagtgat actagaattg tttcatcaca tagctcttcc tccagccttt taaggtcaat
     9601 tctgccatcc tttccattgg gtatccaaga aactcggaag cctttctttt catactcctt
     9661 gattgtattc atcacagctg ggtgttcaat catagaggta atgatatggt tccctctgga
     9721 tttcctagca tccagtgctc cttgaatggc aatattgttg ccttcggttc cacctgaggt
     9781 aaagtatatt tcttcgttct tgacccccag atagctggct atgacacttc gagagtcctt
     9841 catgatcttt tcagcatcca tacccatacg atgcagggat gatggattac cgaaactaat
     9901 cctgagggca tctgtcattt tttctaccac aagatcacgt ggtcttgtgg tagaacagtt
     9961 atctaaataa atcaaattct cacctcttaa tatcccatgt cttcatcaat aatgtataca
    10021 tgtatactgg ttccattggg attcttgctt agaatggttt cagccttgca gatcctgtcc
    10081 ttggaatcac agtcactgca aaccccagta tatacacatg gtgttgtcag attcaacctt
    10141 tttgcattta agggagcggc tactgtttcg attctcttga atgcagcatc cacatccttg
    10201 cacaccttgt ttttcccaac aataacaaat accttcttgt gtccaaaaat catggaggcc
    10261 actctgtttc cagttccatc cttattgacc agcttaccat cttcagtaag ggcatttgag
    10321 ctggtaagat atacatcaga ggttgttgca tattccaagg cagccttttt atcctccgcc
    10381 ttccaatgcc agtatgtatc atttcctctt tccatcagtt cctcatatac gcccatgtca
    10441 agtattgtca tggaacctcc gattccaaca gattcgccta tggctatttc attcagtaac
    10501 ttctccttgg catcctgtgc agtcttgaaa atacttgcag taaaaccatt cttctcaaga
    10561 gccttgataa ctttatccat tgtaaatctc tcctttctca tcatccctat tatactttaa
    10621 atactcatct atttaaactc tattttgtta aatgataaac tttatcattt gtcttgcaat
    10681 gaaataacat atttgttata atctatatga ggttatcccc ctggtagcct caagttagat
    10741 ctctattccc aatacccctt ttgaacggtt atgttccatc ctatattgat aggatgaaag
    10801 gctccaaagc gtggagcctt ctttttttgt tgaaggttct ccttaattgt gttacgattt
    10861 tcatatacta aagggttata ctcaagctga atcgttaaaa ataaatagga ggtggccaga
    10921 tgacaaaaaa gttggcaatt atttttactg gtggaacaat ttcaatgaag atcgatccag
    10981 agatccatgc tgcgatacct gcattgaatg ccgatgaaat tgtaagttcg cttaagaaca
    11041 tagaggatat tgcagaattt gaggtagtca actatgcaaa tcttcccagt ccgcacataa
    11101 ctccagccat tatgatggat atatatcatc ttgtaacaga caggctagct gacccaacca
    11161 tcgatggggt aattgtaact cacggaacag ataccatgga ggaaacagct tatatgctgg
    11221 atcttttaat tgattcagac aagccggtgg taattgttgg agccatgaga aacttctctg
    11281 aacttggata tgatggtgcc agtaatcttt cggcagctat ctgtacagcc gtatctgatc
    11341 tttcaaaagg aaagggtgta cttgtagtta tgaacaatga ggtcaacgcc gccagtcagg
    11401 tcaccaaaac aaacaccctc tccctggata cctttaaatc ccctgaattt ggaccactgg
    11461 gcatagtaga taacgatgag gtcttatact acagaaatat agtttccagg caggtaattg
    11521 aaactgaaaa acttgaagaa aaggttgcac tgatcaagac gggcgcagga atggaatctg
    11581 atattatgga cttttgcatt gataggggct acaagggtat tgtcatagag gctatgggta
    11641 gaggtaatct gccacctgcc atggttccag gcgtcaaaag ggccatccaa aacaatgtcc
    11701 cagtggtaat ggtttcaaga tgcaatacgg gtagagtact ctatacctat ggctacgaag
    11761 gtggcggcag gcaacttagg gacatgggag tcattttagg tggaaatttg cctggtcaaa
    11821 aagccaggat gaagctgatg ctgatcttag gaaagaccag tgactacaat gaggttaggg
    11881 aactgtttga acttaaccta tactgatatc caagggcgcc ctgtcaaggg cgccttatct
    11941 atgagattcc tttttatctc agtctgcttt ccaggtcgtc tatgtcacag ttttcaacct
    12001 ttattgtatg atacttgagg ctctctgtga atttcaaacc tgaagcagtg gcaatgcaaa
    12061 caacttccgc atctgattct atcttccctt cctccattag cctatttgca gcagccaagg
    12121 atgtggccga tgcaggttgg acaaatatcc ctgcattggc caggatcttc tgatgattga
    12181 taatttcatc ctcactgaca tctacagcca ttcccccatt cttcctgatt aaccttaaaa
    12241 cctgattgcc acttggtgga tatggattct caatggcatg agctattgtt ccaaactctt
    12301 ccaactgctg gacttcctcc gatccagaat caaatgctct gactatggga ttgcagcctc
    12361 ttgcctgtga tgctatgaat attggcatcc tatctgtaat tccacttaag tacagctccc
    12421 taaatccctt ttcaattcct ctaaaattcc ccccagcact tgttgggact ataacatagt
    12481 ctggtacctt cattcccagc tgctggaaaa tctcaaagga gcttgtctta taaccctcta
    12541 ccctgtaggg tgcatctgaa ttgatgaaat agataccatt ctcccttccc agtcttatac
    12601 tttctgtata gagctttctg tagttgccct taaccttaat aagccttggc ccatagatcc
    12661 ctatagggcc tatcttctca tctgccattt cctccttaac caggatagtt gcatgaaggc
    12721 cggctcttgc tgcataagct gccacagatg cagccatgtt accagttgag accgtcccta
    12781 cactattgta tcccatggcc ttggcatgga gcaggcctgt aagggttccc ctatccttga
    12841 aggaccaggt tggattcatt ccctcgttct taatatatat tttactgccc ttaacaccat
    12901 tggttccctc ccttatgtga accaatggtg ttgagccttc tccaagactc aatgccaggt
    12961 ccaggtccag gaagggaaag aaggatgcat acctattgaa tattcccttt tccatgggaa
    13021 actcatctgc tattactcca tttgttattg gctcagtttc aagtggctca ccacatactg
    13081 tgcatctacc ggtttcagtc tctaaatcgt atgtttcact acaatttgtg catattagtt
    13141 tttccacaag gaatctcctc ctatttaaat gttttcag
//
LOCUS       DASRQF010000244         2016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_157615, whole genome shotgun sequence.
ACCESSION   DASRQF010000244 DASRQF010000000
VERSION     DASRQF010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157615"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VFD79_06485"
     CDS             complement(<1..718)
                     /locus_tag="VFD79_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HZH93731.1"
                     /translation="MDIVLRLAEELKLKKKHVENVIQMIDAGDTIPFIARYRKEQTGE
                     MTDVTLRDLHNRLVYLRNLEARKEEVIRLVGEQEKLTPELHKEILNADTLQRVDDLYR
                     PFRPKRRTRATMAKERGLEPLAKELMLQAMDREKLVAFASTFIDEENEVPDWETALQG
                     AMDILAEDIADDPDFRRSVKNYLWRNSLIVTQAVDKDADSVYEMYYDFKEPVNKVLNH
                     RILAINRGEKEKVLKASLHVE"
     gene            complement(761..1868)
                     /gene="prfB"
                     /locus_tag="VFD79_06490"
     CDS             complement(join(761..1792,1794..1868))
                     /gene="prfB"
                     /locus_tag="VFD79_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_097668008.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HZH93732.1"
                     /translation="MESIYLDRDRLSEIKETVRELGVSLDLPKLKGRVKELQEESYKE
                     DLWDDVKRAQGIMQEIKAINNEVETYEKLIEDIEDMETMIELIIEEDDFSHYREVQKN
                     IDILERRTDEFKISTLLSGEFDQKSAILSIHSGAGGLEAQDWAEMLLRMYRRWAEGKE
                     YRVETLDLQADTEGGIKSVTLLVEGFNAYGYLRSEKGVHRLVRISPFDSSGKRHTSFA
                     SIDVYPEIDDDVQVEINEKDLKVDTYRASGAGGQHVNTTDSAIRITHIPTGIVVQCQS
                     ERSQHSNKATAMNMLKAKLVQLQQDENKEKIEDLQGSYSQIAWGSQIRSYVFHPYSMV
                     KDHRTDAEVGDVSRVMDGDIDYFINEYLKHKALQ"
BASE COUNT          414 a          560 c          402 g          640 t
ORIGIN      
        1 cctcaacatg gagagacgcc ttaagcacct tctccttttc ccccctgtta attgccagaa
       61 tcctgtggtt aagcaccttg tttacaggct ccttgaagtc gtagtacatc tcgtatacgg
      121 aatccgcatc cttgtctaca gcctgggtca caataaggga atttctccac aggtagttct
      181 tcaccgacct cctgaagtca ggatcatctg caatgtcctc tgcaaggata tccattgctc
      241 cctggagggc tgtctcccag tctggaacct cattttcctc atcaataaag gtggatgcaa
      301 atgccacaag cttttccctg tccatagcct gaagcataag ctcttttgcc aggggttcca
      361 gtcccctttc cttggccatg gttgccctgg tccttctctt gggcctaaag ggtctgtaaa
      421 ggtcatcaac cctttgaagg gtatcggcat tgagaatttc cttgtggagc tcaggtgtta
      481 gcttttcctg ttctccaaca agtcttatga cctcctcttt cctagcctcc aggttcctaa
      541 gataaaccag cctgttatgg aggtccctca gggttacatc ggtcatttca cccgtttgtt
      601 ccttcctgta cctggctata aatggaattg tgtctccagc atcaatcatc tggattacat
      661 tctccacgtg ctttttcttg agtttaagct cttcagccaa ccgcaatact atatccatat
      721 aaaaccaacc tttccaagct ttcttttgtt aaaggccctt ctactgaagg gccttgtgtt
      781 tcaaatattc gttgatgaag tagtcgatgt cgccatccat tacccggctt acatccccaa
      841 cctcagcatc tgtcctgtgg tccttgacca tgctgtaggg atggaatacg taggacctga
      901 tctgtgatcc ccaggctatc tgggaatagg aaccctgaag gtcttcaatc ttttccttgt
      961 tttcatcctg ctgaagttgg accagcttgg cctttagcat gttcatggcc gtagccttgt
     1021 ttgagtgctg tgacctttcc gactgacact ggaccacaat tcctgttggg atgtgggtga
     1081 tccttatggc cgagtcggtt gtgtttacat gctgtccccc agctcccgag gccctgtagg
     1141 tgtcaacctt caaatccttt tcattgatct ccacctggac atcatcgtct atctcaggat
     1201 acacgtcaat gcttgcaaag gatgtgtgtc tctttccaga agaatcaaaa ggggaaattc
     1261 ttacaagcct gtggacaccc ttctcgcttc taagatatcc ataggcattg aagccctcca
     1321 caagcagggt cacagacttt attccaccct ccgtgtcagc ctgtaggtcc agggtctcaa
     1381 cccggtactc cttgccttcg gcccaacgtc tgtacatcct aaggagcatt tccgcccagt
     1441 cctgggcctc caatccacct gcacctgaat ggatggacaa gattgcgctc ttctggtcaa
     1501 attctccgct taggagggtt gaaatcttga actcatctgt ccgtctctcc aggatatcta
     1561 tgtttttctg gacctccctg taatgggaga aatcgtcctc ctctatgata agctcgatca
     1621 ttgtttccat gtcctcgatg tcttcaataa gcttttcata tgtctcaact tcattgttta
     1681 ttgccttgat ttcctgcatt attccctggg cccttttcac gtcatcccaa aggtcctcct
     1741 tgtaggattc ctcctgcagc tcctttaccc ttccttttag tttaggcagg tcaaagtgaa
     1801 acacccaatt ctctgaccgt ctcctttatc tcagaaagtc ggtctctgtc aagatatatg
     1861 ctctccacaa tctcacatcc cttccttatt acacgattta ctgctgtctg gttgggtctc
     1921 catgacactt cttgtatttc ttgccgctgc cacatggaca ggggtcgttt cttccgattt
     1981 tcttttcctt gttgactacg gtcctgtttt caggct
//
LOCUS       DASRQF010000245         4141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_107622, whole genome shotgun sequence.
ACCESSION   DASRQF010000245 DASRQF010000000
VERSION     DASRQF010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107622"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..971)
                     /locus_tag="VFD79_06495"
     CDS             complement(36..971)
                     /locus_tag="VFD79_06495"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HZH93733.1"
                     /translation="MKKAIMLLLGLVLALGLLSGCSTGPEPVQPEGQQELAGRDVLEI
                     MDDFGNTIAFENPPERIISLAPSNTEILFAIGSGDKVVGVTTYDDFPEEVLEIEKIGD
                     FNGINLERIIELQPDLVINYGDGVTEENERLLEAGIQIAGFEPESIDEITDTILRIGE
                     ITGHKQEALDLVDQMTSRKEELLERIQGLEKKTVFYEIWHEPLMAAGPGSFVDQLINL
                     AGGENVAMDAEGDYPQFDLEQLIERNPQVYITASDLPEKTVESIKERPGFGEIDAIKN
                     DRIHLVDGNILSRPGPRIIEALELIMEAIHPELKN"
     regulatory      complement(1063..1247)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(1326..2849)
                     /locus_tag="VFD79_06500"
     CDS             complement(1326..2849)
                     /locus_tag="VFD79_06500"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HZH93734.1"
                     /translation="MKKGSIIAIAVVLVLTVTGMGGYYYLDNYLSGDTFYEGVTIDGR
                     PMGGLTLEEGIHQLQESKEEDLERIIVLKVDGESEYHSNLTLRNLQLEYNIQEVATEA
                     FQMGRRGNVLQRYTEIRNLEKNPVSFNLERSFKLENLAATLEELAAKVDREPIDAKFN
                     FKDGDMEIIEASEGIALDIETFMTDVSNMGEGILDGGTLYIPVKIIQPELEEDYYSKI
                     NGIIGEFSTSFAGSSAGRSHNIRLSAQSFDGMLLMPGQEVSYNRTTGPRQASAGYREA
                     PVIENGELIPGMGGGVCQTSTTLYNALLLADMTILERRPHSIPPAYVPRGTDAAVATG
                     YLDLVFRNDFDYPVLIDTKVEGTRVYFYIYGDQKNRDYSVRISTSQIATTPYRVHENL
                     DNGLEPGARELVQEGRNGYKVNTFKSIVRNGEVVSTQLISQDYYRERDYIYKIGPTPV
                     TEQQIQEPEQVVPQVEEPAQVEEQEQLQEQESANEGDDGLATPDPDQQGAEDPTGSQ"
     gene            complement(2846..3496)
                     /gene="nth"
                     /locus_tag="VFD79_06505"
     CDS             complement(2846..3496)
                     /gene="nth"
                     /locus_tag="VFD79_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HZH93735.1"
                     /translation="MRKRLTKKEKNQVLDILDELYPYARAELNFNNPFELLVATILSA
                     QCTDVRVNKVTQDMFLELKEPMDYINLGEDALEGRIRSCGLSKTKSRNIIGACEKIVS
                     QHQGRVPGSIEELMQLPGVGRKTANVVASNAFGIPAIAVDTHVFRVSNRIGLAASKNV
                     EGTEKDLMKAIPRERWTKAHHQLIFHGRRVCKARNPLCEECQVSTVCLYYMNEVGK"
     gene            complement(3506..3787)
                     /locus_tag="VFD79_06510"
     CDS             complement(3506..3787)
                     /locus_tag="VFD79_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HZH93736.1"
                     /translation="MIIGACSLELHIFEVYSLKEKRQVIKSLLGRLQSRFNISVAEVD
                     DFDIWQSATIGFACVSNESNHVRSMISSVINFVDSDGRVEITNHQIEIL"
     gene            complement(3874..>4141)
                     /locus_tag="VFD79_06515"
     CDS             complement(3874..>4141)
                     /locus_tag="VFD79_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZH93737.1"
                     /translation="KEKMGSLGFDTGHSETPITPVIIGDEAKTMEFSKRLLEKGVFVS
                     GIVFPTVPKGTGRLRCMVTAAHSRAQLDKAVQAFKEVGDEMGIL"
BASE COUNT          988 a         1077 c          763 g         1313 t
ORIGIN      
        1 taatccggat attacccttc atattaatca cagccttaat tctttagttc aggatgtata
       61 gcttccatga tcagctcaag agcctctatg attcttggtc ccggtcttga taggatattt
      121 ccgtcaacca ggtggatcct atcattcttg atggcatcta tttctccaaa tcctggtctt
      181 tcctttatgg attcaacagt tttctcaggc aggtcgcttg cagtgatata aacctgaggg
      241 tttctttcta tcaactgctc aaggtcaaac tggggatagt ccccctcagc atccatggct
      301 acattttctc cacctgccaa gtttatcagc tggtccacaa atgagccagg tccagctgcc
      361 attagtggct catgccaaat ctcatagaac actgtctttt tttcaagacc ctgaatcctt
      421 tccagcagct cctccttccg ggaggtcatc tggtccacaa ggtcaagggc ttcctgcttg
      481 tgtcctgtga tttctccaat ccttaggatt gtgtccgtta tctcatctat gctctctggc
      541 tcaaatccag caatctggat tcctgcctca agcagccttt cattctcctc agtcactccg
      601 tcaccatagt ttatgaccag gtcaggctgg agctcaatta tcctctccag atttattccg
      661 ttgaaatctc ctattttttc aatctcgaga acctcctcag gaaagtcatc ataagttgtg
      721 acacctacaa ccttgtctcc agaacctatg gcaaacagga tttcagtatt gcttggcgcc
      781 agggaaatta tcctttctgg tggattctca aatgcaattg tgttgccgaa gtcatccatt
      841 atttccaaaa catctctccc agcaagctct tgttggcctt cgggctgaac aggttccggt
      901 cctgtgctgc agcctgacaa tagtccaagc gcaagtacaa gtccaagtaa tagcataata
      961 gcttttttca taagtaatct ctcccttttt ttacataaat taaagcccat caggagatgg
     1021 gctattttgg caaaattcat acatccctcc ctccgaggta aagcactggt aaatgtaaca
     1081 ggcagatatc ctgacttacg gttcatcctc agttcagcac cttcccggtt tcccagtggc
     1141 atttttgctg acctcgttcc cgtttacagt agcgggggct gtagtggatt tgcaccactt
     1201 tccctattaa atctcaaatg tgagatacct gtttcaaata ttttttaatt atctaaatac
     1261 atgatatcag aaccacaaga ctaaatcaac ctgtatcacc agttgtgatt tcctatttct
     1321 tcatatcatt gagagcccgt tggatcctca gctccctgct ggtctggatc tggtgttgcc
     1381 aaaccatcat ccccttcatt tgctgattcc tgttcctgaa gctgttcttg ctcttctacc
     1441 tgcgctggtt cctcaacttg ggggacgact tgctcaggct cttgaatttg ttgttccgtg
     1501 acaggagttg gccctatctt atagatgtag tccctctccc tgtaatagtc ctgtgaaatc
     1561 agttgggtac ttaccacttc tccatttctc acaatggatt tgaaggtatt aaccttataa
     1621 ccgtttcttc cctcctgaac aagttctctg gcacctggct ccaatccatt gtccagattc
     1681 tcatgcaccc tgtaaggagt tgttgcaatt tgacttgtgc ttatcctaac tgagtaatcc
     1741 ctgttcttct ggtcaccata gatgtagaag tatacccgtg tgccttcaac cttagtatct
     1801 attaaaaccg gataatcaaa atcatttctg aagacaaggt ccagatagcc agttgcaaca
     1861 gctgcatcgg taccccttgg aacatatgca ggtggtattg aatgaggtct tctctccagg
     1921 atggtcatgt ccgcaaggag aagggcgtta taaagggttg tggaagtctg gcacactcca
     1981 ccacccatcc ctggtataag ctctccattc tcaattacag gtgcttccct gtatcccgca
     2041 cttgcctgcc ttggcccggt ggtcctgttg taggagactt cctgaccggg cataagcagc
     2101 attccgtcaa aggattgggc tgagaggcga atattgtggc ttcttccagc tgaactgcct
     2161 gcaaagcttg tggagaattc cccaatgata ccgttgatct tagagtagta atcctcctcc
     2221 agctcaggct ggattatctt tacaggaatg taaagggtac ccccatcaag gataccctca
     2281 cccatattgg acacatctgt cataaaggtt tcaatatcca atgctatacc ttcagaagct
     2341 tcaatgattt ccatgtctcc atctttgaag ttgaatttgg catctatggg ttccctatca
     2401 accttggcgg ccaattcttc aagggtggct gccagatttt ccagcttaaa gctgcgttcc
     2461 aggttgaaac tgactggatt cttctcaagg tttctgattt ctgtgtacct ctggagtaca
     2521 tttccccttc tacccatctg gaaggcttca gttgcaacct cctggatgtt gtattccagc
     2581 tggagattcc ttagtgttag attgctgtgg tactcacttt caccatccac tttcaacaca
     2641 attatccttt ccagatcctc ctctttagat tcctgaagtt gatggattcc ctcttcaagt
     2701 gtcaatccac ccattggacg accatcaatc gttacaccct catagaatgt atctcctgaa
     2761 agatagttat caaggtaata gtagcctccc attccggtca cagtcaaaac aagtactaca
     2821 gctatggcta ttatacttcc ctttttcatt tccccacctc attcatataa taaagacaca
     2881 cagtacttac ctggcattcc tcacagagag gattcctggc cttgcatact cttctcccgt
     2941 gaaaaatcag ctgatggtga gccttggtcc acctttccct gggtatcgcc ttcataaggt
     3001 ccttctctgt cccttccaca ttttttgaag cggccagtcc aattcgattg gaaaccctaa
     3061 aaacatgggt gtcaactgcg attgcaggta ttccaaaggc attgctagca actacatttg
     3121 ccgttttcct gccaactccc ggcaattgca tcaattcttc aattgacccc ggaaccctac
     3181 cctggtgttg ggacacaatt ttttcacagg ctccaattat attcctgctc ttcgtctttg
     3241 aaagtccaca gcttctgatt ctgccctcca gggcatcctc tcccaaattg atgtagtcca
     3301 tgggctcctt cagctccaaa aacatatcct gggtaacctt gttaaccctt acatcagtac
     3361 attgagccga tagtattgta gctacaagca gctcaaaggg attgttgaaa ttcagttccg
     3421 cccttgcata tggataaagc tcatcaagaa tatccaaaac ctgattcttt tccttctttg
     3481 tcaatctctt tctcatgtct ctctcctata atatctcaat ttgatggttt gttatttcaa
     3541 ctcttccatc ggaatccaca aagtttatga cactgcttat catgcttctc acatgattgc
     3601 tttcattgga tacgcaggca aatcctattg ttgccgactg ccatatgtcg aagtcatcaa
     3661 cttctgcaac ggaaatgttg aatctggatt gaagccttcc cagcaagctt ttaatcacct
     3721 gtcttttttc cttcaacgaa tatacctcaa aaatgtgtag ttcaagtgaa catgccccaa
     3781 ttatcatatt tacacctctt gatgtttgtt atttccatat tatactattt tgtccataaa
     3841 gaaaagaggg gatatcccct cttttctgac gaactataaa attcccattt catctccgac
     3901 ctctttaaat gcctggacgg ctttgtcaag ctgggccctg gaatgggcag cagtcaccat
     3961 gcatcttagt cttcctgttc ccttgggaac tgtagggaat acgatgcctg atacaaatac
     4021 tcccttttct agaagtctct tgctaaactc catggtttta gcctcatccc ctatgataac
     4081 aggtgttatg ggtgtctcac tgtgccctgt gtcaaatccc agggaaccca ttttctcctt
     4141 g
//
LOCUS       DASRQF010000246         7172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1155429, whole genome shotgun sequence.
ACCESSION   DASRQF010000246 DASRQF010000000
VERSION     DASRQF010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7172
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155429"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..743)
                     /locus_tag="VFD79_06520"
     CDS             complement(132..743)
                     /locus_tag="VFD79_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009372343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HZH93738.1"
                     /translation="MHQYKGKLIIHTGSMFSGKTSSLEKEVKRFMIAGYRTVAFKPSV
                     DNRVPTSEIVTHDKTSLEGILVDDIGQILKHCEEGNPEVVAIDEVQFLGGEIEDIIQG
                     VNSLLSKNITVIVAGLDIDFSGRPFEILKELMPIADYLNKHHAVCVKCGTDAWVSHRK
                     TKERERVVIGATKEYEPLCRSCFLKEKKKEEAIVNKNQVTFIE"
     gene            complement(865..1626)
                     /locus_tag="VFD79_06525"
     CDS             complement(865..1626)
                     /locus_tag="VFD79_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93739.1"
                     /translation="MKDKISNEIQRFVADFEKRDGIKSRWKTPLVGFASTMDPMFDML
                     KEVAVPEHLHPRDILADCKTLIAYFLPFQDWIPESNIGKLESSEEWARAYVETNILIG
                     EINDFLIGWLTGMGHETKKLNPSLNMDYENLVSVWSNRHVAYVAGLGTFGMNNMLITE
                     NGCCGRLGNVVTSLELEADKRTGKENCLNKYDGSCTACIDNCVNDSLNLDEFDRFKCY
                     EMLLANGEKYEYLGDASVCGKCLVGLPCSTENPVG"
     gene            complement(1631..2236)
                     /locus_tag="VFD79_06530"
     CDS             complement(1631..2236)
                     /locus_tag="VFD79_06530"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004822724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZH93740.1"
                     /translation="MLKQLMKPENNEEIAIIKTNHGDIKLRLFPEVAPKAVENFTTHA
                     RDGYYDGISFHRIIKDFMIQGGDPDGTGRGGQSIWGRPFEDEFSMEYRNIKGALSMAN
                     AGPKTNGSQFFIVQKGDIENSIIDQMKQLGEGKGFPDDVIQAYREHGGAYWLDGKHSV
                     FGQVFEGIDVVDKIAHLPVGRNDMPKEPVMILTIDVTEYKG"
     gene            complement(2258..2941)
                     /locus_tag="VFD79_06535"
     CDS             complement(2258..2941)
                     /locus_tag="VFD79_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005955491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HZH93741.1"
                     /translation="MIGYPEILARLLISTLLGGIIGIEREASNRPAGFRTHILVSVGS
                     TLIMLVSIYAIPQPADRARIAAQVVSGIGFLGAGTILRTGNHIRGLTTAASLWVCAGI
                     GLAVGSGYYFGGVVTAMIVLISLIVLSKVEKNVLRGANRLIMVTAVDRSGLIGEIGLL
                     FGKHNISIRNISIEDITDDENEPSGLIELKFYIKKPIGFDNRILDNELLQIEGIRSID
                     FDNSGIKEK"
     gene            3037..3390
                     /locus_tag="VFD79_06540"
     CDS             3037..3390
                     /locus_tag="VFD79_06540"
                     /inference="COORDINATES: protein motif:HMM:NF019507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HZH93742.1"
                     /translation="MKEKITCAVCPMACVIDVSLKDGDLLVEGNRCGRGYQFVKKHFE
                     QEDRIVAGRCLLSGGQMSRLPVSTNMKIPAHHVEKALEIIQNTRIQAPVKRGQVIIEN
                     ILETGADVISQRKAL"
     gene            complement(3404..4744)
                     /locus_tag="VFD79_06545"
     CDS             complement(3404..4744)
                     /locus_tag="VFD79_06545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZH93743.1"
                     /translation="MRKELNLTEGNIIKTLTRLALPIMGTSFIQMAYSLTDAMWLGRL
                     STNAVAAAGTGGFFLWLGAALIMITQIGVGVSVSQSYGRSDIKSAREYITVGLLIDIV
                     IGLAYGFCLYTFNSQIISFFNLGDMEVIGMAESYLRIIGMGIIFHFLNPVLSTTLNSS
                     GDSMTPFKVNTIGLVANMILDPLLIFTFGFGIKGAAIATIVSQMAVTVIFLIIGKRID
                     ALYSSFKIFAKPDLKKAVRILKLGLPPSTQMGIHAIISMVLTRIIAGYGPVAVAVQTI
                     GSQIESISWMTSEGFAAAISAFVGQNYGANKISRIKEGYYKGIKVLGTFGIFVSLLLI
                     FAARPLFTIFTPDDPKAIAEGVIYLRILGLSQMFMSVEIGTAGAFNGLGKTIPPTITG
                     VTLNALRIPAAIILSAYTVLGLSGIWWAISGSSILKGLILFAWYQRELHKLKEA"
     gene            complement(4841..5953)
                     /locus_tag="VFD79_06550"
     CDS             complement(4841..5953)
                     /locus_tag="VFD79_06550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZH93744.1"
                     /translation="MINEKRLLERFLKYVQIDSPTKEEREFALALKSELEELGMEVRM
                     DDAGEKVGSNSGNLIGYLKGTTDGEGILFSAHMDTVSPGRGIKPQIKDGVIYSDGTTI
                     LGGDDKAGIAAVVEAIQSIKENKIPHGDIEVAFTIFEEGGLFGSKNLDYSSIRAKKGF
                     VLDSGGDPGTIIVQGPAQNKIDVRFIGKEAHAGVAPETGISAIQMAGEAISKMRLLRV
                     DEDTTANIGSIKAEGPTNIVPKIVEFSAEARSLSDQKLKEQSDHMVKCCQEAAEKFGG
                     SVEVNVETAYGAFKIAEDHPLVNVVKKACGELGIPAETTKSGGGSDTNIFNANGIEAI
                     NLGIGERKPHTLEEHLHIKDLENSARLAIEIIKQFA"
     gene            complement(6023..>7172)
                     /locus_tag="VFD79_06555"
     CDS             complement(6023..>7172)
                     /locus_tag="VFD79_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004819506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HZH93745.1"
                     /translation="AVVVFAGIMSNIASDAGYVVVIPLGAIVFAGAGRHPIAGLAAAF
                     AGVSAGFSANLLIGTLDPLLQGITNEALRSAGMDYEVLPTANYYFMIVSTFLLTFLGT
                     FVTERIVEPNLGEYTGSYKPNKEEITEIESKGLRNAGIALLIFGIVMLALTVPANGLL
                     RSADADGNLTINEFMSNGLIFMILLVFMIPGAVYGKTTGKIKNSNDLVESMTQAMKSM
                     GAYLVLAFFAAQFVSYFGRTNLGTILSVKGADFLESIGFTGLPLIIGFIFISAFLNLF
                     MGSASAKWAIMAPVFVPMMMRLGLSPELTQAAYRIGDSTTNIITPLMSYFAMIVVFAK
                     KYDEESGLGTLIATMVPYSVVFLLGWLLLMVVWYFLGLPLGPGAMIHV"
BASE COUNT         1956 a         1773 c         1276 g         2167 t
ORIGIN      
        1 aacgattacg atcgcttgtt catgacactt ggcgatggtt caaggcagat gaatctaaca
       61 agttcatcgg gaaatataag actctttgaa tagcacttaa acggggatga catttgtcat
      121 ccccacccta tctactctat gaaggttacc tggtttttgt ttactatggc ttcctctttt
      181 ttcttctcct tcagaaagca agacctgcaa agtggttcgt actctttggt tgcacctatt
      241 acaacccttt ctctctcctt ggtctttctg tgacttaccc aggcatccgt accacacttt
      301 acacaaacgg catgatgctt gttcagataa tccgctatgg gcataagctc cttgagtatc
      361 tcaaatggcc tgcctgaaaa gtctatatca agccctgcca caattactgt aatgttcttg
      421 ctcaaaagtg agttgacgcc ctgaattata tcttctatct caccacccag aaactgaacc
      481 tcatctattg ccacaacttc tggattgccc tcttcgcagt gtttaagaat ctgtccaata
      541 tcatctacca gtatcccctc caaggaggtt ttatcatgtg ttactatttc agatgtgggt
      601 accctgttat ccaccgatgg cttaaaggca acagttctgt atcccgcaat cataaatctc
      661 ttaacttcct tttcaaggct tgatgtcttc ccggaaaaca ttgagcctgt gtgtataatc
      721 aatttacctt tgtattgatg catttatgac cctccttata cttgaccata ccattatacc
      781 aagaatttaa ggatagttaa actataagag gcaaaacatg aataaattaa gcccacaatt
      841 acataaaaga ctcatatgag tcttttatcc aactggattc tcagttgagc agggcaatcc
      901 cacaaggcac ttcccgcaaa ctgacgcatc acccagatat tcatattttt ctccgtttgc
      961 caaaagcatc tcgtagcact tgaatctatc aaattcatcc agattcagag aatcatttac
     1021 gcaattgtca atacaggctg tacagcttcc atcatacttg ttgaggcaat tctccttgcc
     1081 ggtcctttta tcagcttcaa gttccaggct tgttacaaca ttgccaagtc gaccgcagca
     1141 tccattctca gttatgagca tattgttcat accaaaggtt ccaagacctg caacataggc
     1201 cacatgcctg tttgaccaaa cgctaaccag gttttcgtag tccatgttca atgaagggtt
     1261 caatttcttc gtttcatgac ccatacctgt aagccatcct atcaaaaaat catttatttc
     1321 gccaatcagt atgttggtct caacatatgc cctcgcccat tcctcagagc tctcgagctt
     1381 cccaatattg ctttctggga tccagtcctg aaatggaaga aaataagcaa taagggtttt
     1441 gcaatctgct agtatatctc ttgggtgcaa atgctcagga acagccacct cctttaacat
     1501 atcaaacatt gggtccattg tggacgcaaa cccaaccaga ggtgttttcc aacgggactt
     1561 tataccatcc ctcttttcaa aatctgccac aaacctttga atttcattag agattttatc
     1621 tttcatgacc ctagccctta tattcagtta catcgattgt caaaatcatt actggttcct
     1681 tgggcatgtc atttctcccc acaggaaggt gtgctatctt gtctacaaca tcaattcctt
     1741 cgaaaacctg gccaaagaca gagtgcttgc catccaacca gtatgcccct ccatgctcac
     1801 ggtaagcttg gatcacatca tcaggaaatc ccttgccttc cccaagctgt ttcatctgat
     1861 caattatgct attttctatg tcgcctttct ggacgatgaa aaattggctg ccatttgtct
     1921 taggtcctgc attggccatt gacaaagcgc ctttgatgtt tctatattcc atgctaaact
     1981 catcttcaaa tggtctcccc catatgctct gacctccacg tcctgttcca tcagggtccc
     2041 cgccttgaat catgaagtcc ttgatgattc tgtgaaagct aatcccatca taatatccat
     2101 ccctggcgtg ggttgtgaag ttctctactg ctttaggtgc aacttccggg aatagtcgca
     2161 gttttatatc accgtggttt gtcttgatta ttgcaatttc ttcattgttc tcaggcttca
     2221 tcagttgctt aagcatttta ctacctccta gtatttgtta tttttcttta atcccgctgt
     2281 tgtcaaagtc aattgaccta atcccttcta tctgaagcag ctcgttgtca agtatcctgt
     2341 tgtcaaatcc aatgggtttc ttgatgtaaa acttgagctc aatcagtccg gaaggttcat
     2401 tctcatcatc ggtaatatcc tcaattgaaa tattccttat actgatatta tgctttccaa
     2461 acaatagtcc tatttcccca atcaatcccg acctatcaac cgctgtgacc atgataagcc
     2521 tattggctcc cctcagtaca tttttttcta cctttgacag tacaataagg gatatcagca
     2581 ctatcatagc agttacaact ccaccaaaat aatacccgga ccccactgca aggccaatcc
     2641 ctgcgcatac ccacaggctt gctgctgttg taagccccct tatgtggttg ccagttctca
     2701 ggattgttcc tgcccccaaa aatcctattc cacttaccac ctgggctgca atcctggccc
     2761 tgtcagcagg ctgaggtata gcatatatgg aaaccagcat tatcaaggta gagcctacac
     2821 ttaccagtat atgggtcctg aatccagctg gtctattgct ggcttctctt tcaattccaa
     2881 ttatacctcc caatagggta gatataagca atcttgcaag tatttctgga tatcctatca
     2941 ttgtactcct ccacaccatg tgaccttatc taaattttac tctaaaactg gcatttcttc
     3001 aactccatat ggtataatct cctggaggtg ttgaatatga aggaaaaaat cacctgtgca
     3061 gtatgcccca tggcatgtgt tatcgatgtt tccctaaagg atggagatct tcttgtagag
     3121 ggaaatcggt gcggcagagg ttaccagttt gtaaaaaaac attttgaaca agaagataga
     3181 attgtagcag gaaggtgtct gctttcaggt ggacaaatga gtagacttcc cgtatctaca
     3241 aacatgaaga taccagccca tcatgttgag aaagcacttg aaattattca aaacacacgt
     3301 atccaggccc cagtgaaaag aggtcaggtc attattgaaa atatacttga aactggagca
     3361 gatgtgatat cacaaagaaa agcactctag aagcctgatt ttatcaggct tctttcagtt
     3421 tgtgaagttc tctttgatac caggcaaata aaattagccc ctttagaata ctacttccac
     3481 ttatggccca ccaaatccct gaaagtccca aaaccgtgta tgctgatagg attatggcag
     3541 ctggaattct tagagcgttt aatgtgacgc ctgttatggt tggaggtatg gtctttccaa
     3601 gtccgttgaa ggccccggct gtaccaatct ccacactcat gaacatctgt gataggccca
     3661 gaattctcag gtagataacc ccctctgcaa ttgcctttgg gtcatctggt gtaaatattg
     3721 taaacaatgg cctggcagcg aatatcaaca ggagtgaaac aaatatccca aatgttccaa
     3781 gtaccttaat ccccttgtaa tatccctcct tgattctgga aatcttgttt gctccgtagt
     3841 tctggcccac aaaagccgat attgctgctg caaatccctc ggatgtcatc caggagatgg
     3901 actcaatctg agatccaatt gtctgtacag caactgccac aggtccatag cctgcaatta
     3961 ttctagtcag gaccatgctt atgattgcat ggattcccat ctgggttgat ggaggaagcc
     4021 caagcttaag aattctcact gcttttttca agtcgggttt tgcaaatatc ttaaaggatg
     4081 agtatagagc gtctattcgc ttgccaataa ttagaaatat tacagttaca gccatctgag
     4141 atactattgt ggcaattgca gcccccttaa tcccaaaccc gaaggtgaaa ataagcaagg
     4201 gatcaagaat catatttgcc accaatccta tggtattaac cttaaagggg gtcatgctgt
     4261 ctccagaact gttgagtgtt gttgaaagca ccggattcag aaaatgaaat attattccca
     4321 tcccaattat acgaagataa ctttcagcca ttccaatcac ttccatatcc ccaaggttaa
     4381 agaatgaaat gatttgactg ttaaaggtat ataggcaaaa tccatatgca agaccaatta
     4441 ctatatcaat caaaagtccc acagttatgt attctcttgc tgattttatg tcagaccttc
     4501 catagctttg agaaacactt acaccaacac caatctgtgt aatcattata agtgctgccc
     4561 caagccacag gaagaatcca cctgttccag ctgctgcaac agcattggtg gatagtcttc
     4621 caagccacat tgcatcagtc agactgtagg ccatctggat gaacgaagtc cccataatag
     4681 gcagcgcaag ccgtgtaagg gtctttatga tatttccttc cgtaagattc agctcttttc
     4741 tcattatgta ctcctagatt ttagtttatc ttcatcattc tacaccattt actggcccat
     4801 tacaacattg aaggatagcc attatggcta tccttcctga ttatgcgaac tgttttataa
     4861 tttctattgc aagtcttgct gaattctcaa gatcctttat atgaagatgc tcttcaaggg
     4921 tatgtggctt tctctcacca attccaaggt ttatggcctc tatgccattg gcattgaaga
     4981 tattcgtgtc actgccacca cctgatttgg tggtttcagc cggaattccc aattctccac
     5041 aggccttctt tacaacattc accaatgggt gatcttcagc aatcttgaat gcaccgtatg
     5101 ccgtttcaac attcacctca acggagcctc caaatttctc tgcagcctcc tggcagcact
     5161 taaccatgtg atctgattgt tccttcaatt tttggtcgct taggcttcta gcctcagctg
     5221 aaaactcaac aatcttaggt actatgttgg taggtccttc agccttgatc gaaccaatgt
     5281 tggcagtcgt atcctcatca actctaagaa gcctcatttt ggaaatcgcc tcacctgcca
     5341 tctggatggc acttattccg gtctccgggg caactccagc gtgagcttcc tttccaatga
     5401 acctgacatc aatcttattc tgggctggtc cctgaacaat aattgtacca ggatctcctc
     5461 cactgtccag gacaaatcct tttttcgccc taatacttga ataatccaag ttcttggaac
     5521 cgaacaggcc accctcttca aaaatggtga atgcaacctc tatgtccccg tgggggatct
     5581 tgttttcctt aatggactgg atggcctcaa ccactgcggc tatccctgcc ttgtcatccc
     5641 caccaaggat agttgtcccg tcagaataga ttaccccatc ctttatctgt ggcttgattc
     5701 ctcttcctgg tgatacagta tccatgtggg cgctgaacag gattccttct ccatctgtgg
     5761 tccccttaag atagcctatc aggttgcctg agttgctgcc aaccttttct ccagcatcat
     5821 ccatgcgaac ttccattccc aactcctcaa gctctgactt caatgccagt gcaaattctc
     5881 tctcttcctt tgttgggctg tcaatctgta catatttcag gaatctttcc agaagtcttt
     5941 tttcatttat catgatctct ccccttccat ctttctattt agaaaaatga ggcttaataa
     6001 taagccccat ttttaccaat cattatacgt gaatcatcgc tccaggtccc aatggcaatc
     6061 caaggaaata ccaaacaacc atcaacaaca accatccaag taggaagacc actgaataag
     6121 gtaccatagt cgcgatcaaa gttcccaaac cactctcttc atcatatttc tttgcaaaga
     6181 ccacaatcat tgcaaagtag ctcataagtg gtgtaatgat gtttgtagtt gagtcaccaa
     6241 tcctgtatgc tgcctgtgta agctctggac ttagtccaag tctcatcatc atcggtacaa
     6301 ataccggtgc cattatagcc cacttggctg atgcagaacc cataaacagg ttaaggaatg
     6361 ctgaaataaa tataaatcca attatcagag gaagacctgt aaatccaata ctttccagga
     6421 agtcagctcc ctttacggaa agaattgtac caaggtttgt tcttccaaag taagaaacaa
     6481 actgtgctgc aaagaatgca agaaccaggt atgctcccat gctcttcatg gcctgagtca
     6541 ttgactccac aaggtcattg ctgttcttta tctttccagt tgtcttacca tacactgctc
     6601 ctggaatcat aaataccaga agaatcataa atataagtcc attactcata aactcattta
     6661 tggtcaagtt accgtcagca tcagctgatc ttagaagacc attggctgga actgttagcg
     6721 caagcatcac aataccgaat ataagcagtg caattcctgc atttctaagt cccttgctct
     6781 cgatttctgt aatctcttcc ttgttgggct tgtatgaccc agtatactct ccaaggtttg
     6841 gctcgacaat tctctcagta acaaatgtac ctaggaatgt caaaaggaag gttgatacta
     6901 tcatgaagta atagtttgct gttggaagga cttcataatc cattcctgca cttcttaaag
     6961 cttcattggt gataccttgt agcaagggat caagtgtacc aatcagtagg ttagctgaga
     7021 aacctgcaga tacccctgca aatgctgcag caagtcctgc gattggatgt cttcctgctc
     7081 ctgcaaacac aattgctccc aaaggaatta ctacaacata gcccgcatca gatgcaatgt
     7141 ttgacatgat acctgcaaat actaccactg ca
//
LOCUS       DASRQF010000247         2354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_331688, whole genome shotgun sequence.
ACCESSION   DASRQF010000247 DASRQF010000000
VERSION     DASRQF010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331688"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /locus_tag="VFD79_06560"
     CDS             <1..1079
                     /locus_tag="VFD79_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZH93746.1"
                     /translation="TSDIDNAVRAGKFVATDLWDMAVRAGMTDVVVDATGVPDTGARI
                     ATTSIMQGKDIVMLNVESDVVVGPYLNRLARQEGVVYTGSAGDEPGAVMELYDFAVAT
                     GFEVLALGKGKNNPLDHHVTPQMVREKAEKSGLKPLRLASFIDGTNTMVEMAAMANAS
                     GFVPDVRGGHGPNTTVEDLPKVYSLKSEGGILNRYGIVDFAFGAAPGVYAIVTTDLPQ
                     VHHEMKFLKMGDGPNYVLFRPYHLTSLETPITIAKAHIYREPSIVPIGDRPVAEVITR
                     AKKDMKKGEYFDGIGEETVYGSIEEYTKAKEEDLLPIGLINRNARAKVDIMKDQFITN
                     GMVELDTNTEIYKLRQLQNQQTTS"
     misc_binding    1124..1332
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1382..>2354
                     /locus_tag="VFD79_06565"
     CDS             1382..>2354
                     /locus_tag="VFD79_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002834999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZH93747.1"
                     /translation="MKRFNELSNEPVKDREQKISDYWKEIDLLHKSVESRDPENPYVF
                     YEGPPTANGKPGIHHVMARTLKDLTCRYKTMQGYQVKRKAGWDTHGLPVEIEVEKKLN
                     LHNKQDIENMGIKDFNEACRESVFTYEKLWREMTERMAYLIDLDDPYITLDNDYIESV
                     WWILDKFFKEGYIYEGHKILPYCSRCGTGLASHEVALGYEEIKTETVTVKFKVKDKDE
                     YILAWTTTPWTLPSNVCVTVHPDLTYIKVKKDEEVYYLSKDLADKVLGEDYEILEEMP
                     GKDLEYMEYDQLIPYIDVKGKAFYVTLGDYVTTEDGTGIVHSAPAFGE"
BASE COUNT          732 a          493 c          626 g          503 t
ORIGIN      
        1 atacaagtga tatagacaat gcggtaagag cagggaaatt tgtggccaca gacctttggg
       61 acatggccgt aagggccgga atgacagatg ttgtggtgga tgccacagga gttccggata
      121 caggtgcaag gattgcaaca acctcaatca tgcagggaaa ggacatagtc atgctaaacg
      181 tggaatctga tgttgtggtc ggaccctacc ttaaccgtct tgccaggcag gagggagtgg
      241 tctatacagg ctctgcagga gatgagccgg gggcggtcat ggaactgtac gattttgccg
      301 tggccacagg ctttgaagta cttgcccttg gaaagggcaa gaacaatccc ctggatcatc
      361 atgtgactcc gcagatggtc cgtgaaaagg ctgaaaaatc ggggctcaag ccactgaggc
      421 ttgcatcctt catcgacggt acaaatacaa tggttgaaat ggctgcaatg gccaatgcgt
      481 caggctttgt tccggatgta aggggaggac atggtccaaa cacgactgtg gaggacctgc
      541 ccaaggttta cagcctgaag agtgagggtg gaatcctgaa caggtatgga atagttgact
      601 ttgccttcgg agctgcccct ggggtctatg caatagtcac gacagacctt ccccaggtcc
      661 accacgagat gaagtttctc aagatggggg atggaccaaa ctacgtgctc ttcaggccat
      721 atcatctcac aagcctggag acacccataa ctatcgcaaa agcccatatc tacagggaac
      781 catcaatagt cccaattggt gacaggcctg ttgcagaggt cataacaagg gccaagaagg
      841 acatgaagaa gggcgagtac tttgacggca ttggtgagga gactgtctat ggaagcatag
      901 aggagtatac aaaggccaag gaggaggacc tcctgccaat cggactgata aacagaaacg
      961 ccagggcgaa ggtggatatc atgaaggacc aattcatcac aaacggcatg gttgaacttg
     1021 atacgaacac tgagatttac aagcttagac agcttcaaaa tcagcaaaca acttcttgac
     1081 aaaagcctgt aaatcctata tattattaaa caagtgtaaa caaataaagg ctatgatcag
     1141 gacagtagat aatccggacc attctagaga ggtggcagcg aggtggaatg tcgccatgga
     1201 aggattattg aagatcacct gtaagcttga cacctgaaaa cagtaagggg tcacgtgaag
     1261 acgttaaact ttaatgaagc gacaggagag atcctgtaat ttgggtggta ccgcggaatt
     1321 ttcgtcccta agtctaattg acttagggat ttttgcattt tacaaggagg agagatgata
     1381 agtgaaaagg tttaatgaat tatcaaatga accggtaaag gatagggaac agaaaatatc
     1441 cgactactgg aaagagattg accttctgca caaatcagtg gaatcaagag atccggaaaa
     1501 cccatatgtt ttctacgaag gacctcccac tgccaacggc aagcctggaa tccatcacgt
     1561 tatggccagg actttgaagg acctgacatg cagatacaag acaatgcagg gctaccaggt
     1621 aaagagaaag gctggatggg acacccatgg actgcctgtt gagatcgaag tggagaagaa
     1681 gcttaacctt cacaacaagc aggacattga aaatatgggc atcaaggatt tcaacgaggc
     1741 atgtcgtgaa tccgtattca cctacgagaa gctttggagg gaaatgacgg agagaatggc
     1801 ctacctgatt gacctggatg acccatacat cacactggac aacgactata tcgaatctgt
     1861 atggtggatt ctggacaagt tcttcaagga aggctacatc tatgaaggtc acaagatact
     1921 tccatactgc tcaagatgcg gtacaggcct tgcatcacac gaggttgcac ttgggtatga
     1981 ggagataaag acagaaacag taacagtcaa gttcaaggta aaggacaagg acgagtacat
     2041 ccttgcatgg acaacaaccc catggaccct cccttcaaac gtatgcgtca ctgtacaccc
     2101 tgaccttacc tacatcaagg tcaagaagga cgaagaggta tattatctgt ccaaggacct
     2161 ggcagacaag gttcttgggg aggactatga aatacttgag gaaatgccgg gcaaggacct
     2221 ggaatacatg gagtacgacc agcttattcc atatatagac gtaaagggca aggccttcta
     2281 cgtaaccctt ggagactatg taacaactga ggatggaact ggaatcgtcc actcggcacc
     2341 ggcatttggt gagg
//
LOCUS       DASRQF010000248         4499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1399232, whole genome shotgun sequence.
ACCESSION   DASRQF010000248 DASRQF010000000
VERSION     DASRQF010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399232"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..1305
                     /locus_tag="VFD79_06570"
     CDS             280..1305
                     /locus_tag="VFD79_06570"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HZH93748.1"
                     /translation="MKMFKSDNTSGVHPKIMEAIINENQGYAHAYGEDDTTKRAEEKI
                     GELLGCDADVFFVTTGTAANVIGLMGLLRTYEGIVAAETAHINVDECGAMEKFAGNKI
                     IQVPNREGKIYIEDIEPLLEVIGDEHSVQPKVISISQTTEMGTLYTAEEIRELADFAH
                     RNNMLLHIDGARIANAVEALGTTLKEMITDTGVDLLSFGGTKNGMMMGEAIVSLNREF
                     GRGYPFHRKQGMQLVSKMRYISAQFIAYLQDDLWIHNARNANQMGKYLAERISDQDGV
                     ELYAPVSTNMVFAFMDKGLIDYLSKKFKFYVTDPSQGQVRFVTSYDITKEDVDLFVDN
                     IIEYNNK"
     gene            1457..1669
                     /locus_tag="VFD79_06575"
     CDS             1457..1669
                     /locus_tag="VFD79_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005397819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HZH93749.1"
                     /translation="MKKTYQLETLTCPSCTMKLEGMLKQTKGVKDYQVLFNSSKIKLE
                     YDENLVDSETIKNNIARLGFEVLKEK"
     gene            1783..2460
                     /locus_tag="VFD79_06580"
     CDS             1783..2460
                     /locus_tag="VFD79_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZH93750.1"
                     /translation="MKCNCHERENQNCIELVPIFSNLTKEEMFEIAMITSEKSLEKGE
                     SIYHAGDRHDSLYVIHKGRVKITRFSESGKEQILRILGPGQFMGELTLFSKELTKDNA
                     VAMEKTIMCKIDSQDLKGLMSRFPSIALKILEELSQRLEKAETLVEDISLSSVEKRIA
                     KSIVDMEKDGLVQLHMTKGDWASHLGMSQESLSRKLRQLQEAGIIRLEGHRKIHVLDF
                     DSLEKIE"
     gene            complement(2475..3035)
                     /locus_tag="VFD79_06585"
     CDS             complement(2475..3035)
                     /locus_tag="VFD79_06585"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HZH93751.1"
                     /translation="MKSIEIMVKEHEHIRRMLKVLREVSYRIMTGGEYDLDDLPEMID
                     FVRNYADKLHHGKEEDILFEAMNAQIEKLAKSGAITGMYIEHDQGRLFIANLESGYKA
                     FKEGDDRARLNIIGNAIAYTDLLDRHIEKENTALYKFAENMLPDEIKTKVDSECDKVE
                     SEALNAGTQARYLNMLQLMEDKYRPE"
     gene            3125..4315
                     /locus_tag="VFD79_06590"
     CDS             3125..4315
                     /locus_tag="VFD79_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HZH93752.1"
                     /translation="MSFKITDKVKWVGKIDWELRKFHGDEYSTNKGSSYNSYLVRDEK
                     TVLIDTVWAPFAKEFVDNLKKEIDLNEIDYIIAQHGEVDHSGALPLLLKEIPDTPVYC
                     TKNGKKILQGHYHEDWNFVEVKTGDTLDIGESTLTFVEARMLHWPDSMFTYMSGENIL
                     FSNDAFGQHYASEFFFNDKVDQCELWEECIKYYANILTPFSKLVTMKIKEVLGFELPL
                     SMIATSHGIIWRDNPAQIVEKYLEWADNYQENQISIIYDSMWHGTRRMAEGIMEGIRK
                     TDPEVTVKLINAAITDKNDIITEIFRSKGILVGSSTVNKSIQHAVGGLMEMVKGLGFQ
                     NKKGAAFGSYGWSGESIEILEGKLKEANFEIVQDGIKALWNPDDEAMDECRDFGKRFA
                     ESFK"
BASE COUNT         1414 a          841 c         1094 g         1150 t
ORIGIN      
        1 ggcaatggcg actcctgcct tgatacccag ggattgggca gcatttgttg ctccggcatt
       61 tctgttgcca atggtcctga tgtcaacctt tttaaccact ttcccataga tatatgcaaa
      121 ctgcagacaa ccaaacagat agcctatgat tattatggcc atgtaggaaa gcattggata
      181 tcaccccttt catctagaat tatactatat tataataagg taaaatagag aaaataaaac
      241 atttgttgta taatggaaga aacgatgggg ggaataatga tgaaaatgtt caagagcgat
      301 aacacttcag gtgttcatcc aaaaataatg gaagctatca taaatgaaaa ccaaggctat
      361 gcccatgcat atggagagga cgataccaca aagagggctg aagaaaaaat aggtgagctt
      421 ttaggctgtg atgctgatgt attctttgta acaacgggaa cagctgcaaa tgtaatcgga
      481 cttatgggac tcttaagaac ctatgaggga atcgtagctg cagagactgc acacataaat
      541 gtggatgagt gcggggccat ggagaagttt gcaggaaaca agatcatcca ggtgccaaac
      601 agggaaggca agatttatat tgaggacata gagccacttc ttgaagtgat aggagatgag
      661 cactctgtcc aacccaaggt gatttctatt tcccagacaa cagagatggg gaccctttac
      721 acggctgaag agataaggga acttgctgat tttgcccaca ggaacaatat gctgcttcat
      781 attgacgggg ccagaattgc aaatgctgtg gaggccctgg gtacaacatt gaaggagatg
      841 atcactgata ctggtgttga ccttctaagc tttggaggaa caaaaaacgg aatgatgatg
      901 ggtgaggcca tcgtatcact gaacagggaa tttggaagag gctacccctt ccacaggaag
      961 cagggaatgc agttggtatc aaagatgcgt tacataagcg cccagtttat tgcatacctg
     1021 caggatgacc tttggatcca taacgcaagg aatgccaacc agatggggaa ataccttgca
     1081 gaaaggatat cagaccagga tggggtggag ctttatgctc ccgtaagcac aaacatggta
     1141 tttgccttta tggacaaggg tctcatagat tacctttcaa agaagttcaa gttttacgtt
     1201 actgatccat cacagggcca ggtaaggttt gttacctcct atgacatcac caaggaggat
     1261 gtggacctct ttgttgacaa tataattgag tacaacaaca aataacttaa ataaaaatgc
     1321 aaaccagctg accacagacc ttcccatgga agggggtgtg gttatatctt ttcaatgaaa
     1381 tttgacctag gtcaaagctt ttttgggtaa tatatcctat aattgaatca ataagaaaac
     1441 atgaggaggt aatgtaatga agaaaacata tcaactggaa acacttactt gtccatcctg
     1501 taccatgaag cttgagggaa tgcttaagca gaccaaggga gtcaaggact accaggtgct
     1561 tttcaactca tccaagataa agctggagta cgatgaaaat cttgtggatt cggagaccat
     1621 aaagaataat attgcaaggc ttggatttga agtgttaaag gaaaagtaat aacggctagg
     1681 ttggcattct ggttggattt gacaagaata taatgggtat catctcaata tgaagcaaag
     1741 gtcagggaca ggaaaaaaag tgctacagac tggagatttg ttatgaaatg caattgccat
     1801 gaaagggaaa accaaaactg catagagctt gtgccaatat tcagcaacct gacaaaggaa
     1861 gaaatgtttg agatcgccat gatcacatca gagaaaagcc ttgaaaaagg tgaatcaatc
     1921 tatcatgcag gtgacagaca tgacagcctt tatgtgatcc acaagggaag ggtcaagatc
     1981 accagattca gcgaatcagg aaaggaacag atccttcgga ttcttggacc gggacagttc
     2041 atgggagagc ttactctctt ctccaaggaa ttgacaaagg ataatgcagt ggccatggaa
     2101 aagacaataa tgtgtaaaat agacagccag gatcttaagg ggcttatgtc aagatttcct
     2161 tcaattgccc tgaagatttt agaggagctt agccagaggc tggaaaaggc agagaccctt
     2221 gttgaggata taagtctttc atcggtggaa aaaaggattg ccaagagtat tgttgatatg
     2281 gaaaaggatg gacttgttca gctccatatg acaaagggtg actgggcttc acatcttggc
     2341 atgagccagg agtcattgag caggaagctg cgacagcttc aggaagcggg aatcataagg
     2401 cttgagggac acaggaaaat ccatgtcctt gactttgaca gtcttgagaa aattgaataa
     2461 agcccttttg ggctctattc cggtctgtat ttgtcctcca taagctggag catgttaaga
     2521 taccttgcct gtgtaccagc attaagagct tcgctctcga ccttgtcgca ttcggagtca
     2581 acctttgttt tgatttcatc tggaagcatg ttttcggcaa atttgtaaag ggctgtattt
     2641 tccttttcaa tatgcctgtc cagaaggtct gtgtaggcaa tcgcatttcc gatgatatta
     2701 agtctggcac ggtcatcgcc ttccttgaag gccttgtagc ctgattctag atttgcaatg
     2761 aaaagccgac cctggtcgtg ctcaatatac atccctgtga ttgcacctga ttttgcaagc
     2821 ttctcaatct gggcattcat ggcttcaaag aggatgtcct cctccttgcc gtgatggagc
     2881 ttgtcagcat agtttcttac aaaatcaatc atttcaggaa ggtcatccag gtcgtattcc
     2941 ccaccggtca ttatcctgta ggagacttcc ctaaggacct tgagcatcct gcggatgtgt
     3001 tcgtgttcct tgaccattat ttcaatagat ttcaagttat cacctcaatc tgttatttgt
     3061 agttgggttt ttaccccaaa taagtggggt ttaacatata cacaataaaa aggagggttt
     3121 ttcaatgagc tttaaaatta cggacaaggt taaatgggta ggtaagatcg actgggaact
     3181 gaggaaattc catggtgacg agtattcaac aaacaagggt tcttcctaca actcatacct
     3241 tgtaagagac gagaagactg tcttgataga caccgtgtgg gctccctttg caaaggagtt
     3301 tgtggacaat cttaagaagg aaatcgacct taatgagatt gactacataa tcgcccagca
     3361 tggagaggtg gaccacagtg gagcgcttcc actccttctt aaggaaatac cagatactcc
     3421 tgtgtattgt acaaagaacg gaaagaagat actccaggga cactatcatg aggactggaa
     3481 ttttgtagag gtaaagacag gggatacatt ggatataggt gaatcaacct taacctttgt
     3541 ggaggcaagg atgcttcact ggccagacag catgttcact tacatgagcg gtgaaaacat
     3601 cctgttcagc aacgacgcat tcggtcagca ctatgcctct gaattcttct tcaatgacaa
     3661 ggtagaccag tgtgagttgt gggaagaatg cataaaatat tatgccaaca tccttacacc
     3721 attcagcaag cttgttacta tgaaaatcaa ggaagtactg ggatttgagc tgccactgag
     3781 catgattgca acctctcatg gtataatctg gagggacaat cctgcacaga ttgttgagaa
     3841 gtacctggag tgggctgaca actaccagga aaaccagata tccataatct atgactcaat
     3901 gtggcacgga accagaagga tggctgaggg aataatggaa ggtatcagaa aaaccgatcc
     3961 tgaggtaacc gtaaagctca taaacgcagc cataacagac aagaacgata taataactga
     4021 aatcttcagg tcaaagggaa tccttgtggg atcatcaact gtcaacaaga gcatacagca
     4081 tgctgttgga gggctgatgg aaatggtcaa gggccttgga ttccagaaca agaaaggggc
     4141 agcatttggt tcatatggat ggagcggaga atccatagag attcttgaag gcaagcttaa
     4201 ggaagcaaac tttgagatcg tccaggacgg aatcaaggca ctgtggaatc ctgatgacga
     4261 agccatggat gaatgcagag actttggaaa gagatttgcg gaaagcttca agtagactta
     4321 tggcggggga gaccccgcta ttttgtttac ccgtgtaaaa gttaatattc agggtaacat
     4381 tactcatata gatgccacct acttaaatta ttaatagtga gaagaaagaa gggagagata
     4441 tgaagattgt tattgtacat tgcggccttg gggaggtttt aactcctgag aagacggtc
//
LOCUS       DASRQF010000249         2241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1016410, whole genome shotgun sequence.
ACCESSION   DASRQF010000249 DASRQF010000000
VERSION     DASRQF010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016410"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VFD79_06595"
     CDS             <1..692
                     /locus_tag="VFD79_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZH93753.1"
                     /translation="SSINLILALFNMFPAFPLDGGRVLRAAIWHFTGNLHRATRISST
                     IGQWFGYFLMFIGLTNMFYGNLINGLWILFIGYFVVQASQSSYQNTIVSDIFSRTMVG
                     EFMTRDVVAINRAVSVRDAVDEYFFRYKYNVFPVEDEEGVIGLITIDNVKRIPKDEWG
                     VTSLGAIVTELGEGIVVSPDETVSNVISKIFNNHIGRVLVMEEGRLVGIVSRTDIMNH
                     LRIYMQLNNIK"
     gene            724..1728
                     /gene="galE"
                     /locus_tag="VFD79_06600"
     CDS             724..1728
                     /gene="galE"
                     /locus_tag="VFD79_06600"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HZH93754.1"
                     /translation="MKVLVTGGLGFIGSHTVHMLLQGGHQVLVLDNLSNSKIETLDRI
                     NSMTQNNTRFIQGDVLDDKILDKALSTFGIEGVVHFAALKSVKESIESPERYHLNNVV
                     GTERLISKCLEHNIRKFIFSSSAAVYGSAKAPVREDAEMGEALNPYGSTKVLCEELLK
                     ETHRVHGRPDMTILRYFNVAGAHPTGNLGEDAGPDGGNLMSHIIRAAGDGKMYIYGDD
                     YDTPDGTAIRDYIHVMDVARGHILALERQKSGINTYNLGTGKGTSVLGMVNTFQEVNG
                     VRVSYEIVARRKGDVGVSCADPSKALDDLGWRAELSLGDMVRDSWNHEKRKLAIDTCI
                     "
     regulatory      1781..1964
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
BASE COUNT          636 a          431 c          593 g          581 t
ORIGIN      
        1 tgtcatccat caaccttatt ctggcactct ttaacatgtt tccagccttt cccctggatg
       61 gagggagggt ccttcgggct gccatatggc atttcacggg gaatctgcac agggccacaa
      121 gaatttcctc tacgatcggc cagtggtttg gttatttcct tatgttcatt ggtcttacaa
      181 acatgttcta cggtaatctc atcaacggct tgtggatcct attcatagga tattttgtgg
      241 tccaggcctc ccaatcaagc taccagaaca caatcgtgtc cgatatattc agcaggacca
      301 tggtgggaga attcatgacc agggatgttg tggcaataaa cagggctgta tctgttaggg
      361 atgctgtgga tgagtatttt ttcaggtaca agtacaatgt gtttcccgtg gaggacgagg
      421 aaggagtcat tggcctgatt acaatagaca atgtgaaaag gatccccaag gatgagtggg
      481 gagtcacaag tcttggagcc attgtcactg agcttggtga aggtattgtg gtatctcccg
      541 atgaaaccgt atccaatgtc ataagcaaga tattcaacaa ccatatcggt agggtcttgg
      601 ttatggagga gggtaggctg gtgggaattg tctcaagaac cgatattatg aaccatctta
      661 gaatctatat gcagcttaac aatatcaagt gatataatgg tatatatcgg aggtggtcat
      721 ggagtgaagg ttcttgttac agggggattg ggttttatag gaagccacac agttcatatg
      781 cttctccaag ggggacacca ggttcttgtc ttggataatc tttcgaactc aaagattgaa
      841 acacttgata ggataaacag tatgacccag aataatactc ggtttatcca aggggatgtt
      901 ctggatgata aaattctaga caaagcccta tccactttcg ggattgaagg agtggttcat
      961 tttgctgccc ttaagtcagt gaaggagtcc attgaaagcc cggaaagata ccatctcaac
     1021 aatgttgttg gaacggagag gctaatatca aaatgccttg aacacaatat aagaaagttt
     1081 atcttcagct cctcagctgc ggtctatgga tctgcaaagg ctcctgtaag ggaggatgca
     1141 gaaatgggtg aggcccttaa cccttatgga agcacaaagg ttctgtgtga agaactccta
     1201 aaggaaaccc acagggtcca tgggagaccg gatatgacaa tcctcaggta cttcaacgtt
     1261 gctggagccc atccgacagg gaacctgggt gaggacgcag gccctgatgg aggaaacctg
     1321 atgtcgcaca taatcagggc cgctggcgac ggaaagatgt acatatacgg agatgactac
     1381 gacactccgg atggaactgc catcagggac tacatacatg ttatggatgt tgccagggga
     1441 catattctgg cactggaaag gcaaaaaagc gggataaaca catataacct ggggactggc
     1501 aagggcactt cagtcctggg tatggtcaat accttccagg aagtcaacgg tgtaagggtc
     1561 tcctatgaga ttgttgctag gcgaaaaggt gatgttggtg tttcctgtgc cgacccgtcc
     1621 aaggcccttg atgacctggg ctggagagct gagttgagtc ttggtgacat ggtgagggac
     1681 tcctggaatc atgaaaaaag aaaacttgct attgacacat gtatttgatg tgtgttaata
     1741 tcttcaacaa tagcaactaa ataaaaatcc tcatccctgc gggtgaggta gaggcgcggt
     1801 ttctattagt accaccacag aggacggaat cctgtgaagt gttgggaaag gagcgatcgc
     1861 cgaagcttca ggcaccaaag cctgttgctg ggtctgcatc caagaggtgc aggactgtca
     1921 tcctggtact aagctcggta ccttgatgtt gtgctatctc acataagagc aagaggggag
     1981 agggtaacct atggcattgc catgtagaaa atcacctgac ttaccgtcag gtttttattt
     2041 tttttgaaaa ctgattgaag ggagattatc gagttgatct atgaagaaag agatggaagg
     2101 cttcattttg acgggtgtga cactgtcaac ctgactaaaa aatatggaac gccattatat
     2161 gtatactcat taggggaaat taaagatagg ataggggaac taagggaaag cttcttgaat
     2221 aggtatgaaa acacaagagc t
//
LOCUS       DASRQF010000250        11175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1538043, whole genome shotgun sequence.
ACCESSION   DASRQF010000250 DASRQF010000000
VERSION     DASRQF010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 11175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11175
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1538043"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..313
                     /locus_tag="VFD79_06605"
     CDS             71..313
                     /locus_tag="VFD79_06605"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:209..211,aa:Sec)
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HZH93755.1"
                     /translation="MWNALKEIPYGETRSYKYIAESIEKPKAYRAVGNANNKNPISII
                     IPUHRVIAANGDLAGFGGGLEIKKSLLELEKNDHLV"
     gene            436..1890
                     /locus_tag="VFD79_06610"
     CDS             436..1890
                     /locus_tag="VFD79_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HZH93756.1"
                     /translation="MIKQQQTMILSPYLDIYELVVPKNNLLRQINELIDFSFVYDELI
                     NKYCLDNGRNAISPIRMFKYLLLKSIFDLSDVDLVERSKYDMSFKYFLDMAPEETVIE
                     PSSLTKFRKLRLKDINLLDMLISKTVAIALEKGIIKSKSIIVDATHSKARYNQKSPRE
                     ILQDCSKKLRKSVYEIDPTMRDKFPAKNTVDQLENEIQYCSDLIEIIKKSDNISEYPK
                     VKEKMNLLEETMAEDIEHMQISEDEDAKIGHKTSDTSFFGYKTHIAMTEERIITAATV
                     TTGEKNDGKELETLLIKSKEAGMEVETIIGDAAYSEKGNIELADENNIKLIAKLNPSV
                     TQGYRKKEDEFQFNKDAGMYVCKAGHMAIRKARQGKKRVANNQQDCYYFDVEKCRQCS
                     QKEGCYKEGAKSKTYSVTIKSDIHIGQIEFQESEYFKEKSKERYKIEAKNSELKHRHG
                     YDVASSSGLVAMEMQGALAIFTVNLKRIIKLMGE"
     gene            complement(2037..3323)
                     /locus_tag="VFD79_06615"
     CDS             complement(2037..3323)
                     /locus_tag="VFD79_06615"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HZH93757.1"
                     /translation="MIKDSILIDILKDQVKPALGCTEPAAVALGVARAKEIITEEVLD
                     LHITVNNNILKNGMSVGIPGTNERGLDFAAALALMVGKSQYGLEVFKDVDQESITKAL
                     EIVEAKLIEVELDKTKSNLFIKVVARGESEVATVIIEKAHTNIVFESLDDRILLDSRN
                     SCNMDSNNGNRSDDLKFKIMDYSISELVSFVDSVPCEYLDFIQTGINMNLKLAQVGLM
                     EDLGLGMGKHYMEKSNDVYKIAKAYTVAASEARMTGYPLPVMSSAGSGNHGLVAIIPL
                     SIMGSELGFTEDRVRRAVALSHLVTIFVKVQLGALSPVCGCAVAAGLGCSAGISYLKD
                     GTVEQIEDSIVNMIAGVSGMFCDGAKIGCAYKLGISVDAAVDAASMAMEGMRIPSDNG
                     ILGRTAEESISNLARVSTEGMTNTDPVILDIMMNKC"
     gene            3549..5276
                     /locus_tag="VFD79_06620"
     CDS             3549..5276
                     /locus_tag="VFD79_06620"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZH93758.1"
                     /translation="MSILNSIKSELQHIVEAMYSVTNVDITIVDHNLKRVVGTGIHGN
                     AIGMMAPLNSAFHKCITTGDHYLIENPREEAICWECENKENCGELVEICLPIEYKEER
                     IGVLGLCAFDETAKANLLANTDGYRTFENQLTGIINTMLKEKDYGQLLEYRSNEMFTL
                     INSLNEGVLILDNNHKIASMNSYIKDRLDNGYDISSLGAILPDHTLNQLTEKGFNGEV
                     GPVTVGNGNFIIQSNPIEVDGIKQGYILIFSDLNKMKKSVLKSHKDKDIVTFDHILGE
                     SEAIKQARRQAVQVADSEASVMIIGETGTGKEVFARAIHHGGQRGDQVFMALNCGAIP
                     ENLIESELFGYEQGSFTGASKSGRQGYFEVATNGTLFLDEIGELPYLMQVKLLRALEE
                     KEITRIGGNRGIKVNPRIISATHRNIDSMARKGTFREDLFYRLNIVPIHIPPLRDRGY
                     DVLILGKYFLDHFAGIYGKDLVGFSSDAENLLLKYGFPGNIRELKNLIEYAVIFSTGS
                     KISAENLRGKIPINLIDESKTLSEMTREYERELIRSKIQLLGDDVESKKTLAQKLGIS
                     LATLYRKMD"
     gene            complement(5292..5519)
                     /locus_tag="VFD79_06625"
     CDS             complement(5292..5519)
                     /locus_tag="VFD79_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93759.1"
                     /translation="MASLGLGSVGTAYFANLTKYKPIFVIITGFLLYKSYGLMEKNNA
                     GKTTRIIFWVSAALAILALYYPTILGFFYAS"
     gene            5815..6963
                     /locus_tag="VFD79_06630"
     CDS             5815..6963
                     /locus_tag="VFD79_06630"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93760.1"
                     /translation="MKELDLDVLKELSQMDLNGLVSIYLPTFKQGQEANQGRIRLKNL
                     LRDAEGDLVKSGFSQKDALDLLKPASKLLDETIFWQNQEQGLGIFISPKEMRYHKLPF
                     EVDELAVVSNRFYIRPLIRLFTSIENFNVLALSQAEVRLFEASPYGIEKIEVPEIDEL
                     VENYIPASELHQEATSPKGAASGGGPGMHGYNEMSKTEKNEISKHLRSIDKEVNKVLK
                     ETKHPLIVHSVDYIYPMYREVSSYPNLMEESIKGSPEGLGEKEIHKKAMEIYSPRAEA
                     RLEREVEKFTTLFNTDSKLGTDSIERIVLSSNNGGVDTLFIADDVQKWGSFNEDDNSL
                     DVNNKEDVGQVDLMDKAAMLTLSNGGRVHVLKRDKMPSGKTIAAVLRF"
     gene            7050..7892
                     /locus_tag="VFD79_06635"
     CDS             7050..7892
                     /locus_tag="VFD79_06635"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HZH93761.1"
                     /translation="MKNFVTASWLKDNLSRENLLVFDVRYELGNEDYGQEEYEKGHIE
                     GAIFVPMEEILTGEVKEHGGRHPLPDLKIFASNMESFGVDDGKTIVVYDDGELAMAGR
                     LWWMLKYIGKEEVFILKGGIGGWKKKGFSLTTDEAKPVKGHALSIRVKDEMIADVEDV
                     RKAIVRDDQVVVDSRTPDRYRGEIEPIDRIPGHIPSAVNYPWTQLVEDLSDADSEELT
                     AHYSGLGKFEKILVHCGSGITGTVNIMFMEEVGLKPVLYLGGYSDWISYEDNQVIREA
                     GDNK"
     gene            8045..9529
                     /gene="malQ"
                     /locus_tag="VFD79_06640"
     CDS             8045..9529
                     /gene="malQ"
                     /locus_tag="VFD79_06640"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HZH93762.1"
                     /translation="MKKRESGILLPISSLPGEYGIGDFGKEAYKFVDFLHHARQRYWQ
                     ILPLGVTGYGDSPYQCISSFAGNPYFIDLDEFIEKGYLSKKEILQEKLDADADKVDYG
                     KLYHGKYRLLRLAYERGKEEIWSQLESFYGDNRPWLREFALFMTIKDLQDNRAWQLWD
                     ESLRDFSTYHVNEVESDHKEGFYFWVFTQFYFMKQWEKLKKYANNKGIKIIGDLPIYV
                     SSDSSDLWANPSLFKLDENLEPTTVAGVPPDYFTATGQLWGNPVYDWDAVEREGYGFW
                     LRRIQHSFVLYDTLRIDHFRGFESFWEVPAGSVNAVNGKWVKGPGMKLFNKIREELGD
                     LDIIAEDLGFQTEHVVKLIKDTGFPGMKILQFAFDPDGDSDYLPHNHERNSVVYTGTH
                     DNKTVRDWIDTAPENEKKFAIKYLKLDDVDEGYNWGLIRGAWCSGAYLAVAQMQDFLN
                     LGDESRMNVPATLGNNWTWRVKKSELNQKMSKRIAELTRTYRRG"
     gene            9534..>11175
                     /gene="glgP"
                     /locus_tag="VFD79_06645"
     CDS             9534..>11175
                     /gene="glgP"
                     /locus_tag="VFD79_06645"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005977 - glycogen metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HZH93763.1"
                     /translation="MNHESLKNRIDLYSMTTFGGEFKDLLDSEKYVVLSKAMMETILP
                     HWNQSRKKTETMKKAYYLSSEYLMGRFLTNNLIGMDSKDEVKEILANMGVDYNQIEES
                     EEDPALGNGGLGRLAACFLDSAATLGYPMDGYGIRYDFGLFKQKIVDGEQVEFADNWT
                     KYGDPWGIRKVSETVRVTFKGGEINAVPYDYPVIGYDKKLINTLRLWKAESLEEFDFR
                     AFNDQNYDLAVKAKNDAENIAKVLYPNDSFEEGKKLRLKQQYFFVSASLQDMIRRFKT
                     RNLDFGEFPQYNSIQLNDTHPAVAIPELMRLLTRKEGLEWDKAWEITRETFAYTNHTL
                     LAEALEQWPERYYQDLIPQVYKVIVKIHQMLNEELKTKGIQDHDLNRYEIIHNGNIRM
                     AWMSIFGSKSVNGVARLHTDLLINQELNDWYRLYPEKFNNKTNGITQRRWLLQSNPEL
                     SDLITQLLGSEDWITDLKRLKDLESYGDDPEILKKFLDIKRTKKQQLADYILQEEGEE
                     IDPDSLFDIQIKRLHEYMRQLLNAFHILYLYYELKENPD"
BASE COUNT         3667 a         2097 c         2590 g         2821 t
ORIGIN      
        1 atacctcgaa ggaagtagaa gagattttca gctccccata agcctctcag gaacaccatt
       61 tcaaattaag gtgtggaatg cactaaagga aattccctat ggtgagacga ggagctacaa
      121 gtatatcgct gagagtattg aaaaaccaaa ggcctacagg gctgtaggaa atgccaacaa
      181 caagaatccc atatcaataa tcataccctg acaccgtgta attgcggcca atggtgattt
      241 ggcaggattt ggcggaggtt tggagataaa gaagagcttg cttgaattgg aaaaaaatga
      301 ccacctagtt taatgaggcc actgaaaaag tccttttgac gggattccaa atgaaagcct
      361 tgaaagcact gatttataca gtgttttcaa ggcttttgtg gtataattat agtatcaaat
      421 taggtaggtg atattatgat taagcaacaa caaacaatga tattaagccc ttacttggac
      481 atatatgagt tagtcgttcc taaaaataat cttctaaggc aaattaatga attaattgat
      541 ttttcttttg tttatgatga attaattaat aaatattgct tagataacgg cagaaacgct
      601 attagtccaa tacgaatgtt taaatatctg cttttaaaat ctatttttga tttatcggat
      661 gttgatcttg tagaacgttc aaagtatgac atgtcattta agtactttct tgatatggcg
      721 cctgaagaaa cagtaattga accaagttcc ttgactaaat ttcgtaaact taggcttaag
      781 gatattaacc ttctagatat gttaattagt aagactgtcg ctattgcact cgaaaaagga
      841 attataaaat ccaagtctat tattgttgat gcaacccatt caaaagcaag atataatcaa
      901 aaatcaccta gagaaatatt gcaagattgt tcaaaaaaac tacgtaaatc tgtatatgaa
      961 atagatccaa ctatgagaga taaatttcct gctaaaaaca ctgttgatca attagaaaat
     1021 gaaattcagt attgcagtga cttaatcgaa ataattaaga aaagcgacaa tatatctgag
     1081 tatccaaaag tgaaagaaaa gatgaattta cttgaagaaa caatggcaga agatatagaa
     1141 catatgcaaa tttccgaaga tgaagatgct aagattgggc ataaaacatc agatacttca
     1201 ttctttggat ataagacaca tattgcaatg acagaggaga ggattattac cgcagcaaca
     1261 gttacaacag gtgaaaagaa tgacgggaaa gaactagaaa cattattaat taaaagcaaa
     1321 gaagcaggaa tggaagttga aacaattatt ggagatgcag cgtattccga aaaagggaac
     1381 attgagctag ccgatgaaaa taacataaag cttatagcga aattaaatcc ttctgtaacg
     1441 cagggatacc gtaaaaaaga agatgaattt cagtttaata aagatgcggg aatgtatgtc
     1501 tgtaaggcag gacatatggc aatcagaaaa gcacgtcaag ggaaaaaaag agttgctaat
     1561 aatcagcagg attgctacta ttttgatgta gaaaaatgca gacaatgctc tcaaaaagaa
     1621 ggttgctata aggaaggagc aaaaagcaaa acttattcag taacgattaa aagtgatatt
     1681 catataggcc aaatagaatt tcaggaaagc gaatatttca aagaaaaatc aaaagagcga
     1741 tataaaatcg aagccaaaaa tagtgagtta aaacacagac atggatatga tgttgcatca
     1801 tcttcaggtc ttgttgctat ggaaatgcaa ggtgcattag ccatatttac ggtaaattta
     1861 aaaagaataa tcaagttaat gggagagtaa tgctcacttt acttaataga taacagaaaa
     1921 aatagcggtt aaaaatcaaa tttgactttt aatcgctatt tttgaatcat tttatttagg
     1981 ataattgaaa aactgtaagt ttttcagtgg cctctagttt aatggtggtc atttttttag
     2041 cacttattca tcattatatc caggatgact ggatcagtat tggtcatacc ttctgtggaa
     2101 acccttgcaa gatttgaaat tgactcctct gcagttcttc ccagaatacc gttgtctgag
     2161 gggatcctca taccctccat agccatggag gctgcgtcca cagctgcatc aacagagatt
     2221 ccaagcttgt aggcacagcc aatctttgcg ccatcacaga acatgccgga cacacctgct
     2281 atcatgttta caattgaatc ttcaatttgt tccacagtgc catccttaag gtaacttatg
     2341 ccagcactgc aacctaggcc agctgcaaca gcacagccac atacaggact caaagcccca
     2401 agctggacct tcacaaaaat tgtaacaaga tgactcaagg ccacggccct tctaacccta
     2461 tcttcggtga agccaagctc agaacccata atggaaagtg gtattattgc aaccaaacca
     2521 tggtttcccg acccagcact actcattact ggcaggggat agcctgtcat ccttgcctcg
     2581 gaggcagcca ctgtgtaggc ctttgcaatc ttataaacat catttgactt ctccatgtag
     2641 tgcttgccca ttccaaggcc caaatcctcc atcaatccaa cctgagccag cttaaggttc
     2701 atattgatcc cagtctggat gaaatcaaga tattcacaag gcacactatc tacaaaactc
     2761 acaagctcac tgatggaata atccatgatc ttgaatttca gatcatcaga cctgtttccg
     2821 ttgttagaat ccatattaca gctatttctt gaatccagaa gaattctgtc atcaagggat
     2881 tcaaaaacaa tatttgtgtg ggccttttca attatcacag tggcaacctc agactctccc
     2941 cttgcaacaa ccttgataaa caggttggac ttggtcttgt caagctcgac ctctataagc
     3001 tttgcctcaa caatctccag tgcctttgtg atgctttcct ggtcaacatc cttgaatacc
     3061 tcaagaccat actgggactt gccaaccatt agggcaaggg ctgcagcaaa gtccagtccc
     3121 ctttcattag tccccggaat tccaacactc atgccatttt tgagaatatt gttgttaact
     3181 gttatatgta ggtccaagac ctcttctgta ataatttcct tggccctggc aacccccaag
     3241 gcaaccgctg caggctcggt acatcccaga gccggtttaa cctggtcttt aaggatatca
     3301 ataagtatgc tgtctttaat catcatatca cctcaaccct atatttctgc aagttccatg
     3361 ccaagaaact caccagatat caatgggcat tctaggcatt ttcttgtcct tggggaaaag
     3421 tttggattgt aatattttct caataatgat aatctttaca tatagaaaag ctagaaacct
     3481 gatatatagg tagtttgcaa aattctcaaa catgagaaca aattatcaaa aatgagaaag
     3541 gtgatgttgt gagtatacta aatagtatta agtctgagct gcagcacatt gttgaggcca
     3601 tgtattcggt taccaatgtt gacattacaa tagttgacca taaccttaaa agggttgtgg
     3661 gaaccggtat tcacggaaat gccattggga tgatggcgcc actgaactct gccttccaca
     3721 agtgcataac aactggagac cattatctta tcgaaaatcc tagagaggag gcaatctgct
     3781 gggagtgtga aaacaaggag aattgcggag agcttgtgga gatttgcctg ccaattgagt
     3841 acaaggagga aaggattgga gttctggggc tctgcgcatt tgatgagact gcaaaggcaa
     3901 accttctagc aaatactgat ggctacagaa cctttgagaa ccagcttacg gggattatca
     3961 acactatgct taaggaaaag gactatggac agctcctcga atacaggtcc aacgaaatgt
     4021 tcaccctaat aaattcccta aatgaaggtg tcctaatcct tgataacaat cacaaaatag
     4081 cttccatgaa cagctacata aaggataggc tggacaatgg gtatgacatt tcaagccttg
     4141 gagcgatact tccagaccat actctgaacc agctgactga gaaaggtttc aatggagagg
     4201 ttggtccagt taccgtggga aacggaaact tcataataca aagcaatccc atagaggtgg
     4261 atggaataaa gcagggctat atattgattt tcagcgacct gaacaagatg aaaaaatcgg
     4321 tcctaaagtc acacaaggac aaggacattg taacctttga ccatatactt ggagagagcg
     4381 aggcaataaa gcaggcaaga cgccaggctg tacaggtagc tgacagtgag gcttcggtga
     4441 tgataattgg agaaacagga acaggcaagg aggtatttgc cagggccata caccatggag
     4501 gtcaaagggg agaccaggtg tttatggcct tgaattgtgg agcaatacct gagaacctta
     4561 ttgaaagtga actattcggc tatgagcagg ggtcttttac aggtgccagc aagtcaggaa
     4621 ggcagggtta ctttgaagtt gctaccaatg ggactctatt tctggatgaa atcggagagc
     4681 tgccatatct tatgcaggta aagctcttaa gggcattgga ggaaaaggaa atcaccagaa
     4741 tcggtgggaa cagggggatc aaggtgaatc ccaggataat ttcagccacc cataggaata
     4801 tcgactccat ggcaaggaag ggaaccttca gggaggacct gttctacagg ctaaacattg
     4861 tacccatcca cattccacca ttgagggata ggggctacga tgtcttgatc cttgggaaat
     4921 actttctgga ccactttgcc ggaatctatg gaaaggacct ggtgggcttc agttctgatg
     4981 ccgagaacct cttgcttaag tacggatttc caggaaatat cagggagctt aaaaacctca
     5041 ttgagtatgc agtgattttt tcgacagggt caaaaataag cgccgagaat ctaaggggaa
     5101 agataccaat caatctgatt gatgagtcaa aaacactaag tgaaatgacc agggaatatg
     5161 agcgtgagct tatcaggtcg aaaatccaac tccttggaga cgatgtggag tccaaaaaga
     5221 ccctggcaca aaagctgggg ataagtcttg ctacccttta tcgaaagatg gactaaaaga
     5281 aaaaacgcct cctaggaggc gtagaaaaat ccaagtatag ttggataata tagtgccaaa
     5341 attgccagtg ctgcggaaac ccagaaaatt atcctagtgg ttttcccggc attgtttttt
     5401 tccattaaac cgtaggattt atatagcaga aacccagtga taatgacaaa aattggcttg
     5461 tatttcgtta aattagcaaa atatgcagtt cctacagatc ccagacccag ggatgccaaa
     5521 atcaggcctc cacttcagca aagggatgct gcaactgctg aaaaaattga gcctacactc
     5581 atcagttttt ccttcatcga gacacctcca aacccacagg gggtatagtt gtaatcccat
     5641 attaacataa aaacccataa ggttcaaatg aactttgatc catttttcag aatacccctt
     5701 ccaattgttt tgaatcactc aatagtggta atagtatagt aacagaaaga ttgagtgatc
     5761 ttaacgcgac actatcactt gtcgcgtgga aataacaatg aaaggggttt atctatgaaa
     5821 gaattggacc ttgatgtatt gaaggaattg tcacagatgg acttaaatgg actcgtctcc
     5881 atttatttac ctactttcaa acaaggacag gaggcaaatc agggcaggat aagattaaag
     5941 aacctcttaa gggatgccga gggggatctt gtgaagagtg ggttttcaca aaaggatgct
     6001 ttggaccttc taaagccggc ttctaaactg ttggacgaaa ccatattctg gcagaaccag
     6061 gagcagggtc ttggaatatt catttctcca aaagaaatga gataccacaa gctacccttt
     6121 gaggtggatg agttggcggt ggtttccaat cgattttaca ttaggccatt gataaggctt
     6181 tttacctcta tagagaactt caacgtactg gcactaagcc aggcagaggt tagactcttt
     6241 gaagccagtc catatgggat agaaaaaata gaagttcctg aaatagatga attggtggaa
     6301 aactatatcc ctgccagcga gctgcaccag gaggccacaa gtccaaaggg agccgcatcc
     6361 ggcggtggac ctggaatgca cggatataac gagatgagta agactgagaa gaatgaaata
     6421 tccaagcacc ttcgaagcat agataaggaa gtcaacaagg ttctcaagga gaccaagcat
     6481 cccttgatag tgcattcagt tgactatatt tatccaatgt acagggaggt aagctcatac
     6541 cctaacctga tggaggaatc aatcaagggt agcccggaag gtcttgggga aaaggaaata
     6601 cacaaaaagg ccatggaaat atattcccct agggcagagg ctagattgga aagggaagtt
     6661 gagaagttta ctactctttt caacacagat tccaagcttg gaacagacag catagaaaga
     6721 attgtactgt catccaacaa tggaggagtt gacactctgt tcatagcgga tgatgtacag
     6781 aagtggggaa gcttcaacga ggatgacaac agtcttgatg taaacaacaa ggaggatgtt
     6841 ggccaggtgg atcttatgga taaggcagca atgctaacac tatcaaacgg aggaagggtc
     6901 catgtcctca aacgggacaa gatgccttca ggaaaaacca tagctgcagt attgagattc
     6961 tagagacctg aatcaaatta gggtacactg ataatcttgt gtaccctttt gctttttaga
     7021 ccaaaataca gtggtgggag gaatttgata tgaaaaattt tgttacagca agttggctca
     7081 aggacaattt gagcagagaa aaccttctgg tattcgatgt tagatacgaa ttggggaatg
     7141 aagactatgg tcaggaggaa tacgagaagg gtcacataga aggggcaata ttcgtaccaa
     7201 tggaggagat cctcacagga gaggtaaagg aacatggcgg gagacatccc ctgcctgatt
     7261 tgaaaatatt tgcatccaac atggaaagct ttggggttga cgatggtaag accatagttg
     7321 tatatgacga tggtgagctg gccatggcag gtagattatg gtggatgctt aagtatattg
     7381 gcaaggagga ggtcttcata cttaaaggcg gaataggagg atggaagaag aagggattta
     7441 gcctgaccac agatgaagcg aaaccggtta agggtcatgc cttgagtata agggtaaagg
     7501 atgaaatgat agcggatgtt gaggatgtga gaaaagccat agttagggat gaccaggttg
     7561 ttgtggattc caggactccc gataggtaca ggggagagat tgaaccaatt gacagaatac
     7621 ccggccacat accatcggca gtgaattatc catggaccca gctggtagag gacttgtctg
     7681 atgctgatag tgaagaactc acagctcact acagtgggct gggaaagttc gaaaaaatac
     7741 ttgtacattg tgggtcaggg attacaggta cggtcaacat aatgtttatg gaggaggttg
     7801 gacttaagcc ggtgctttac ctgggaggct acagtgactg gatcagttat gaagacaacc
     7861 aggtaataag ggaagcaggg gataacaagt aagaggattc acaccatacc atcaggaagg
     7921 taggcatcca aatgagggta tggttttctt gattgtttac aagccacccg gtttggggta
     7981 atatagtact atgttcaaac ggttgcacaa acatttatat ttccaggaaa gagggtgaga
     8041 aagtttgaaa aaaagagaaa gtggaatatt gcttcccatt tcctccctgc caggagaata
     8101 cggtattggg gactttggga aggaagcgta taaatttgta gactttttac atcatgcaag
     8161 acaaaggtac tggcagatac ttcccttggg agttacaggt tacggggatt ctccctacca
     8221 atgcatctct tcatttgctg ggaacccata ttttattgat ctggatgaat ttattgaaaa
     8281 gggttacctg tcaaagaaag agatactcca ggagaaactg gatgctgatg ctgacaaggt
     8341 ggactacggc aagctatacc atgggaaata cagacttctt cgcctggcct acgaaagagg
     8401 gaaggaagag atttggtcac aactggagtc cttctacggg gacaacagac catggctaag
     8461 ggaatttgca ttgtttatga ccataaaaga ccttcaggac aacagggcat ggcagctatg
     8521 ggatgagagt ttaagagatt tttccaccta ccatgtaaat gaagttgaaa gtgaccataa
     8581 ggaagggttc tacttttggg tattcactca gttttatttc atgaagcagt gggaaaagct
     8641 taagaagtac gcaaataata aaggaattaa aataatagga gacctgccca tatatgtatc
     8701 ctctgacagc tctgaccttt gggccaatcc gtcacttttc aagctggacg aaaaccttga
     8761 gccaactaca gtggcaggag taccaccaga ttatttcaca gcaaccggtc agctttgggg
     8821 aaatcctgtg tatgattggg atgcagttga aagggagggc tatggtttct ggctaaggag
     8881 aatacagcat agcttcgtgc tttacgatac attgagaata gatcatttca ggggatttga
     8941 atcattctgg gaggttccag ccggttctgt aaatgcagta aacggcaagt gggtaaaagg
     9001 tcctggaatg aagctgttca ataagataag ggaagagctt ggagacctgg acataatagc
     9061 ggaggatctc ggttttcaaa cggagcatgt cgtaaagctt ataaaagata ctggatttcc
     9121 aggtatgaag atactccagt ttgcctttga cccggatggt gacagcgact accttcccca
     9181 taatcacgag aggaattcgg ttgtctacac cggaacccat gacaacaaga ccgtaaggga
     9241 ctggatcgat acagcgcctg aaaatgaaaa gaaatttgca attaaatacc tcaagctgga
     9301 tgatgtggac gagggataca actggggtct aatcaggggt gcctggtgtt caggagcata
     9361 tctggcagtt gcccaaatgc aggattttct taatctgggt gacgaatcaa ggatgaacgt
     9421 tcctgccacg ttgggaaata actggacatg gagggtgaag aagtcggaac taaatcagaa
     9481 aatgtccaaa aggattgcgg aactgaccag gacatacagg aggggttaat ttgttgaatc
     9541 atgaaagttt aaagaataga attgatttgt attccatgac aacctttgga ggtgaattca
     9601 aggatcttct tgactcagag aagtacgtag tattgtccaa ggccatgatg gagacaattc
     9661 ttccacactg gaaccaatcc agaaaaaaga ctgaaaccat gaaaaaggca tactatcttt
     9721 catcggaata ccttatgggt agattcctta caaacaacct tattggcatg gacagtaagg
     9781 atgaagttaa ggagatacta gccaacatgg gagtagacta caatcagata gaggaatctg
     9841 aggaggatcc agcacttggc aatggaggtc tgggaagatt ggcagcctgc tttttggatt
     9901 ctgctgcgac actgggctat cccatggatg ggtatggtat caggtatgat ttcggcctgt
     9961 tcaagcaaaa aattgttgat ggtgaacagg tagagtttgc agacaattgg accaaatacg
    10021 gtgacccatg gggaattaga aaggtcagcg aaacggtacg ggttaccttc aagggtggag
    10081 agataaatgc tgtaccctat gattacccgg taataggtta tgacaagaag ctgataaaca
    10141 ccctgagact ttggaaggct gaatccttgg aggaatttga cttcagggcc tttaacgacc
    10201 agaactacga ccttgcggtt aaggcaaaaa atgatgcgga aaacatcgcc aaggtcctat
    10261 accctaatga ctccttcgaa gaaggcaaga agctaaggct aaagcagcag tactttttcg
    10321 tttctgcctc cctacaggat atgattagaa ggttcaagac caggaatctg gattttgggg
    10381 aattcccaca atacaattca atccagctga acgatacgca tcctgcggtt gcaatacctg
    10441 agcttatgag gctattgacc aggaaggaag gcctggagtg ggataaggca tgggaaataa
    10501 caagggaaac atttgcatac acaaaccaca cactgcttgc agaagccttg gaacagtggc
    10561 ctgagaggta ttaccaggac ctgattcccc aggtgtacaa ggtaatcgta aagattcacc
    10621 aaatgctcaa tgaagagctt aagacaaaag gaatccagga ccatgacctt aacaggtacg
    10681 aaataatcca taatggaaac atcagaatgg cctggatgtc aatatttggt tccaagtcag
    10741 tcaatggagt tgcaagacta cacacggacc ttctcatcaa tcaagaactc aatgactggt
    10801 ataggctcta tccagagaaa ttcaacaaca agaccaatgg aatcacacaa agaaggtggc
    10861 tacttcagtc aaatcccgag ctgtctgacc tgattaccca gctcttgggg tcagaggact
    10921 ggataacgga ccttaagagg ttgaaagacc ttgaatccta tggggatgac cctgagattt
    10981 tgaaaaagtt cctggatatc aaaaggacca agaagcagca gctcgccgac tatatacttc
    11041 aggaggaagg ggaggaaata gaccctgatt ccctgtttga cattcagata aagagacttc
    11101 atgaatacat gagacagctc ctaaacgcct ttcacatact ctacctatac tatgagttga
    11161 aggaaaaccc cgata
//
LOCUS       DASRQF010000251         4847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1037028, whole genome shotgun sequence.
ACCESSION   DASRQF010000251 DASRQF010000000
VERSION     DASRQF010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037028"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VFD79_06650"
     CDS             complement(<1..309)
                     /locus_tag="VFD79_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HZH93764.1"
                     /translation="MKETKEMINDTDLWAGLNKPDLTVDEADIVVFGLAYDGAASFRH
                     GAKDGPKSIRNITYSITPTTEDFELIDNLKVLDLGDYEDTDQVRLFENVRERVKELVD
                     K"
     gene            468..941
                     /locus_tag="VFD79_06655"
     CDS             468..941
                     /locus_tag="VFD79_06655"
                     /inference="COORDINATES: protein motif:HMM:NF024055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HZH93765.1"
                     /translation="MKSLIIYCSDYRGNTEEIAKVIADEIDGRLVNVKELKGETIQTD
                     DYELIGFGSGIYRESMSHRLYRLVEDMDLEGKDTFVFSTSGVGMKLYNNRLTRLLEKK
                     GANVRGSFACKGSFTASDFTEKRIFNIFGKKSQGHPDTRDRERARSFIGSVIKSL"
     gene            993..1709
                     /locus_tag="VFD79_06660"
     CDS             993..1709
                     /locus_tag="VFD79_06660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HZH93766.1"
                     /translation="MEVMWSQHTPYIEPFDMQHLIYIGILVSILALMIRNRDSLRENR
                     KLYGMVILGLSVFQQILLYTWYWMEMGFNLSESLPLHICRISTLLGIWFLISKNHKVL
                     DVMFFFGLYAYGSFLYPSRVYPVQHAIGISFVLNHAITILLPWFGHIAYGWIPNKEGL
                     KRSMVLFLGYFLFVYLLNPIIDGNYFYLKYRPFFTKWPEHIYVPFIVSFTLAGFYFAY
                     WAADRFLGSESYNMETGEAD"
     gene            1761..2951
                     /locus_tag="VFD79_06665"
     CDS             1761..2951
                     /locus_tag="VFD79_06665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HZH93767.1"
                     /translation="MIDYIGLAKEINNEIIQNRRRIHQFPETGFELDRTLEFVEEKLK
                     EYGIEPRRVGRSGITATIGKEGKTILLRADMDALPMKEETGLEFASGNEFCHSCGHDA
                     HTAMLLGAARLLKEQEESLGGTVKLMFQPAEELLAGALDMIEAGILENPKVDAALAMH
                     IYVGNELSRTGTISYARGPALFSGDAIKIHVKGKNAHGSTPDKGIDAISIAAHIVIAL
                     QQIIAREVPTDDQAVVIVGKISGGDTVNTLAGSADLEVSVRATTEKMREYLKKRVKEI
                     SENVAVTFGGKAEVEFWYGMGPLFNDLDLTDEIVGYCKEVIGEENLNKIPIAVGTEDF
                     TSVAGKVPGVMINLGVGSIDEGYSLSIHNPGMVLNEEALHVGAALYAQASTRYLMERE
                     KQED"
     misc_binding    3024..3275
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3304..4197
                     /locus_tag="VFD79_06670"
     CDS             3304..4197
                     /locus_tag="VFD79_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZH93768.1"
                     /translation="MGIFKKEMFIVTMQRKGLMALLMILAVSMLMAGCTASAGVEQEA
                     TNRLEEIKERGYMEVVMEPYFAPYEFIDPSKDGVDKYVGSDVEFAKYIADELGVELKI
                     IPLEFSAVLSSVTEGKYDMVISALSFTPARAEAMNMSKGYFFPEGGAGHGLLIRAEDV
                     DSIAGPEDISDKVIVAQSGSLQEMFVNDQVPGYKEFKRVSATTDGFLMVQENKADVCA
                     ASIPMAQLYIDANPEANLMVVENFEFYMDESTSGTRIGMPKDEDELTQEINRIIDKVI
                     ESGEFAQWHKDFTEYAKSLGL"
     gene            4238..>4847
                     /locus_tag="VFD79_06675"
     CDS             4238..>4847
                     /locus_tag="VFD79_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HZH93769.1"
                     /translation="MISTIIQLWTKYDYVYLNGLVGTLWLAAVTVFVATILGTLLAIM
                     KLSKIKLLDRITNLYIGILRGTPILLQLYFFWILLPKISPVTLSDTACIVIALIVNAS
                     SYVAEIIRAGIQAVDKGQTEAAKSLGMSNRNTMRRIILPQAIKNILPALGNQYINMVK
                     QTSLASVFFVQELMTSYRTVQSATFLAIPSLVIVGIIYLVVTT"
BASE COUNT         1453 a          869 c         1227 g         1298 t
ORIGIN      
        1 cttgtccacc aattctttca ctctttccct gacattctca aagagtctca cctggtcagt
       61 atcctcataa tcacccaggt ccaaaacctt aagattgtca ataagctcaa agtcttcagt
      121 ggtgggtgtt attgaatagg tgatgttcct tattgatttt ggaccatcct ttgctccatg
      181 gcgaaaggag gcagctccgt catatgctag tccaaacacc acaatatctg cttcatcaac
      241 tgttagatca ggtttgttca accctgccca tagatcggtg tcatttatca tttccttggt
      301 ttccttcatt gtctcaactc ctccattttt atgtatgaac atattctatc acatatttga
      361 atgccaatcc tcagaattca tcaaacagga gactttttga ctataccttg ctataattgt
      421 taccataggc tggtatttga atgccaattg agtttctgga ggtaagtatg aaatccttga
      481 taatatattg ctctgactac agaggaaata ctgaagagat agcaaaggtg attgcagatg
      541 aaattgacgg aagacttgtg aatgtaaagg aacttaaggg tgaaacaata caaactgatg
      601 attatgaact gataggcttt ggatcaggta tttacaggga aagcatgtcc catagattgt
      661 ataggttggt ggaagatatg gatcttgagg gcaaggacac ctttgtattc tcaacaagtg
      721 gagttggaat gaagctttat aacaataggc tgacaagact tttggagaag aagggagcaa
      781 atgtcagggg aagctttgcc tgcaagggta gttttacagc ctctgatttt acagaaaaga
      841 ggatattcaa catctttggg aagaagtccc agggtcatcc tgacaccagg gacagggagc
      901 gggcgagaag cttcataggt agcgtaataa aaagcctgta aaatatgtct ataattttag
      961 gttgatgtag ggataattgg gagagtgata atatggaggt catgtggagt cagcacacgc
     1021 catatataga gccttttgat atgcagcatt taatatacat aggtatcctt gtaagcatac
     1081 tggcgcttat gattagaaac agggattccc ttagggagaa tcgcaagctt tatggcatgg
     1141 tgattcttgg attatcagta tttcagcaaa tacttctgta tacctggtac tggatggaaa
     1201 tgggattcaa tctttcagag tcactgccac ttcacatatg caggatttcc acgctgcttg
     1261 ggatatggtt tctgatttca aaaaaccata aggtgctgga tgtgatgttt ttcttcggct
     1321 tatatgcata tgggtcattt ctatatccat cgcgagtcta tccggttcaa catgccatag
     1381 ggatatcatt tgttttgaat cacgccatta caatactact accttggttt gggcatattg
     1441 cttatggctg gattcccaac aaggaaggct tgaagaggtc aatggtattg ttcctgggat
     1501 acttcctatt cgtgtacctt ctgaatccaa taattgatgg aaattacttt tacttgaagt
     1561 acaggccctt ctttaccaag tggccggagc atatatatgt accatttata gtatcattta
     1621 ccttggcagg gttttatttt gcatactggg cggcggaccg tttccttgga agtgagagct
     1681 acaacatgga gacaggagaa gctgactgat gtattttaaa aataaaggat gcaactacaa
     1741 tccaatacag gaggagagaa atgattgatt atatcgggct ggcaaaggaa ataaacaatg
     1801 agataattca aaacagaaga aggatccacc agttccccga aacggggttt gagctggaca
     1861 ggactctgga gtttgttgaa gaaaagttaa aggagtacgg aattgaacca agaagggttg
     1921 gaaggagcgg aatcaccgca acaataggaa aagaaggaaa gaccattctt ttgcgggcgg
     1981 atatggatgc tcttccaatg aaggaggaaa ccggtttgga atttgcatca gggaatgaat
     2041 tctgtcattc ctgtggccac gatgcccata cagcaatgct tcttggtgct gcaaggctgt
     2101 tgaaggagca ggaggagagc cttggtggaa ctgttaagct gatgttccaa cctgcggagg
     2161 aacttctggc tggagccttg gatatgatag aggcaggaat ccttgagaat ccaaaggtgg
     2221 atgcagcctt ggccatgcat atatacgttg gcaatgaact atccaggacc ggtacaatat
     2281 cctatgccag aggaccagcc cttttctcgg gagatgccat caagatccat gtcaagggaa
     2341 agaatgctca tgggagcact ccagacaagg ggatcgatgc cataagcata gctgcacata
     2401 tagtaatagc attacagcag atcattgcgc gggaggtacc aaccgatgat caggccgtag
     2461 tcatagttgg caagataagc ggtggggata cggtaaacac tcttgcagga agtgcggacc
     2521 ttgaggtgtc ggtcagggca acaactgaga agatgcgaga gtacctgaag aagagggtca
     2581 aggaaatatc agagaatgtt gcagtcacat ttggtggcaa ggcagaagtg gagttttggt
     2641 atggaatggg tccacttttc aatgaccttg acttgactga tgaaatagtt ggatattgca
     2701 aagaagtcat aggtgaggag aatctgaaca agattccaat agcggttgga acggaggact
     2761 tcacatcagt tgcgggcaag gtaccaggag tcatgataaa cctcggtgtt ggaagtattg
     2821 atgagggcta cagcctatca atacacaatc caggcatggt cctgaatgag gaggctctac
     2881 atgttggagc tgcactgtat gcacaagctt ccacaagata tctaatggag agagaaaaac
     2941 aggaagatta aaaaaaagaa ttttcatttt agggtttaca aagattgtga aacatgttat
     3001 tgttataaat attaatttaa tatgctaatg cgatgacagg aaagagtagt cgatgtgaat
     3061 gcctaaagag agtacatggt tggtgtgaat gtatggcagt cctttgatga acacatcctg
     3121 gagcaggtgg atgaaagcct tttggtaagt agttttattc ggattgctgc aaccgttata
     3181 aatgcagggg tatagaagct tgtacctgat gagggtaatg tcgcgaggca ttatcaaatt
     3241 aaggtgggac cacgggaata actctcgtcc tttagtatag actattggac gggagttttt
     3301 taattgggaa tttttaaaaa ggagatgttt attgtgacaa tgcaaagaaa aggtttaatg
     3361 gcattattga tgattctagc ggtatcaatg cttatggcag gttgtaccgc cagtgcagga
     3421 gtggagcagg aagcaacgaa cagactggag gaaataaagg aaaggggata catggaggtt
     3481 gtcatggagc cctattttgc tccctatgaa tttatcgatc caagcaagga cggagtggac
     3541 aagtatgtgg gaagtgatgt tgagtttgcc aagtacatag cagatgagct tggtgtagag
     3601 cttaagatta tacccttgga gttttctgct gtgctaagca gtgtaacgga agggaaatac
     3661 gatatggtca tttcagccct atccttcaca cctgcaaggg cggaggccat gaacatgtcg
     3721 aagggatact ttttcccaga gggcggagca ggtcacggac ttctgataag agcagaggat
     3781 gtggattcaa tcgcgggtcc tgaggatata tcagacaagg ttatagttgc acaaagcggg
     3841 tctttgcagg agatgtttgt aaacgaccag gttccaggat acaaggaatt caagagggta
     3901 tcagcaacaa ccgacggctt tctcatggtt caggaaaaca aggcagatgt atgtgcagca
     3961 tcaattccaa tggcccagct ctatatcgac gcaaatcctg aagctaattt gatggtagtt
     4021 gagaattttg agttttacat ggatgaatca acatcaggaa ctaggattgg aatgccaaag
     4081 gacgaggatg agttgaccca ggagataaac aggattatcg acaaggtaat tgaatccgga
     4141 gagtttgccc agtggcacaa ggattttaca gagtatgcga aaagccttgg gttatagggt
     4201 ggaatcagga atagatttga cacaaaggag ataaacaatg ataagtacaa taattcagct
     4261 ttggacaaag tatgattacg tatacctgaa tggactggtg gggacactct ggcttgcagc
     4321 agtaacagta tttgttgcca ccatactggg aactcttctt gcgatcatga agctttctaa
     4381 aatcaagctt ttggacagaa tcacaaactt atacattgga atattaagag gaaccccaat
     4441 attgcttcag ttatacttct tctggattct tctgccgaag atatcccctg tcacactgtc
     4501 agacaccgcc tgcattgtca tagccctcat agtcaatgcc agttcatatg tggcggagat
     4561 aataagggct ggaattcagg ccgttgacaa gggacagacg gaggcggcca agagcctggg
     4621 aatgagcaat agaaacacaa tgagaaggat catactgccg caggccataa aaaacatact
     4681 gccagcccta ggcaaccagt atataaacat ggtgaagcag acatccctgg cctcggtatt
     4741 ctttgtccag gagctgatga cctcctacag gactgttcaa tcggcaacct ttctggcaat
     4801 accttccttg gtcatagtgg gtatcatata ccttgtagtg accactg
//
LOCUS       DASRQF010000252         3415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1432949, whole genome shotgun sequence.
ACCESSION   DASRQF010000252 DASRQF010000000
VERSION     DASRQF010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432949"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VFD79_06680"
     CDS             complement(<1..748)
                     /locus_tag="VFD79_06680"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HZH93770.1"
                     /translation="MSGRIIFHIDVNSAYLSWEAAYRLQHGSQTDLRDIPSIVGGDQE
                     KRHGIVLAKSIPAKKFGIITGETIHSAREKCPGLVMVPPNYYRYIMASDAMVHLLQDY
                     SPLIQRYSIDEVFLDYSNMDTPFMDAAREMSKRIENELGFTVNIGIGPNKLLAKMASD
                     FSKPNKIHTLFHHQMEEKMWSLPVQDLFMVGSRTARKLNGRGIFTIGHLASLDRDYIH
                     SWLKKPGLLIWEYANGIENSQVRSGGTPIKS"
     gene            781..1026
                     /locus_tag="VFD79_06685"
     CDS             781..1026
                     /locus_tag="VFD79_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93771.1"
                     /translation="MITEQVFEVKAEKFSLTNKKMKKPAESCIMEKRYKRGVERDEGA
                     VYKTGNKQKEKNDFTLKATGAPGACLPCKPAAGFAAG"
     gene            947..2059
                     /locus_tag="VFD79_06690"
     CDS             947..2059
                     /locus_tag="VFD79_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93772.1"
                     /translation="MISPLRRQEPLELAYLANQLQDSLQDKVEIVFIDMELERGSFKK
                     HLLEIQPSLVVFWGDQGQEDLVDDQASMVKDISPESYVALAGELSEGEGSPYVDFLFR
                     KNPVEALNETVLGIITERSLQEINRNVATVENETIDQPLREADRGIFRKYQDRYYFLQ
                     YGGTVDVTSQYRLEFDEMGLIGKKDNPLRPLEDVAKDVESLVGASVYLKDRDIWDDPK
                     RLKRLLELLKERNLQREYISVGNWRTILENESLLEDFAGLGLKVLLMEMDFPKDQDDW
                     IFKGEVLRMIRKHGVEPAIIIREKMVKEDQDALSFWLKDRKEGLVLLEGEAFEENRPI
                     YTHASISTWAFLKWTKDHGLGEARRRQKEYKNTLLI"
     gene            complement(2083..2502)
                     /locus_tag="VFD79_06695"
     CDS             complement(2083..2502)
                     /locus_tag="VFD79_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZH93773.1"
                     /translation="MAKTRLDANITGNMGWEMDINGHHFVTDAAIEAGGNDKGPRPKA
                     LLLAGLIGCTGVDVKMILDKMKVDLQDMKISAEAEAAEDHPKVYTDIHLTFTFKGKDL
                     PLDKLEKAVKLSQEKYCGVTAMLQKAAPVTYDIVVEE"
     gene            complement(2550..3317)
                     /locus_tag="VFD79_06700"
     CDS             complement(2550..3317)
                     /locus_tag="VFD79_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZH93774.1"
                     /translation="MLKVRNLWKSFNSDTDFQIDVFRGFNLDIEVNKCTAIIGPNGCG
                     KSTLLNLVSGSLPVDQGQIILNGEDITSLPEEKRALNLGRVHQSPSMGVSPSLTILEN
                     LSLANKKGESFSLRSLVKKKNLEMFVSMLRDLDLGLEDKLHTQVKYLSGGQRQSLSLI
                     MAAMKHPDLLLLDEHTAALDPKTSHVVMEKTRELLDKYNITTMMVSHHMGDALKYSQR
                     IIMLDKGKIILDKPSCDVSEKELLDIYKAKLAERIIA"
BASE COUNT          916 a          739 c          830 g          930 t
ORIGIN      
        1 ctgactttat gggtgttcct ccgcttctaa cctgggagtt ttcaatcccg ttggcatact
       61 cccagataag aagtcccggc tttttaagcc aggaatggat gtagtccctg tccagggagg
      121 ccaaatgacc tattgtgaat attccccttc catttagctt tcttgcggtt ctggatccaa
      181 ccataaaaag gtcctgaacg ggaagactcc acattttctc ctccatctgg tggtggaaga
      241 gggtgtggat cttatttggc ttggaaaagt ccgaggccat ctttgcaaga agcttgttgg
      301 gacctatccc tatgttcaca gtgaaaccca gctcattctc tatccttttg gacatttccc
      361 ttgcagcatc cataaaggga gtatccatgt ttgaatagtc cagaaaaacc tcatctattg
      421 aataacgctg aataagggga gagtaatcct gaagaagatg gaccatggcg tcactggcca
      481 taatatacct gtaatagttt gggggcacca taaccaggcc gggacacttc tccctggccg
      541 aatggattgt ctctccagtg attatgccga atttctttgc agggatggat ttggcaagga
      601 ctatcccgtg ccttttttcc tggtctcccc ccacaatgga gggaatgtcc ctaaggtcgg
      661 tctgagaccc gtgctgaagc ctgtaggcag cctcccagga aagatacgct gaatttacgt
      721 ctatatggaa aatgattcta cctgacatgg aaaacacctc ttatgtattt ctgtgattca
      781 atgataacag aacaagtgtt cgaagtcaag gcggagaaat tctcccttac caataaaaaa
      841 atgaaaaagc ctgctgaatc ctgtataatg gagaaaagat ataaaagggg agttgaacgg
      901 gatgaaggtg ctgtttataa gaccgggaac aagcagaagg agaaaaatga tttcaccctt
      961 aaggcgacag gagcccctgg agcttgccta ccttgcaaac cagctgcagg attcgctgca
     1021 ggataaggtg gaaatcgtat ttattgatat ggagctggaa agggggtcct ttaaaaaaca
     1081 ccttcttgaa atccagcctt cccttgttgt gttttgggga gaccagggtc aggaggacct
     1141 tgtggatgac caggcctcaa tggtcaagga cataagcccc gaatcctatg tggccctggc
     1201 cggtgagttg tcggaagggg aggggtcacc ctatgtggac ttccttttca ggaagaatcc
     1261 agttgaagcc ttaaatgaaa cggtccttgg aataataacc gaaagaagcc tccaggagat
     1321 aaaccgaaat gtcgcgacag tggagaatga gaccatagat caacccctaa gggaagcgga
     1381 tagggggata ttcagaaaat atcaggatag gtactacttt cttcagtacg gaggcactgt
     1441 ggatgtaacc agccagtaca gactggagtt tgatgagatg ggtcttattg gaaagaagga
     1501 caatccattg aggccattgg aggatgttgc aaaggatgtg gaatcccttg tgggggcatc
     1561 cgtctacctt aaggacaggg atatttggga tgacccaaag agattgaaga ggcttttgga
     1621 gctgttgaag gagaggaacc ttcaaaggga atatatatcc gtagggaact ggaggactat
     1681 acttgaaaat gaatctcttc tggaggattt tgcaggactt ggacttaagg tcctgttgat
     1741 ggaaatggat tttccaaagg accaggatga ctggatcttc aagggtgagg tcctaaggat
     1801 gataagaaag catggagttg agccggccat aattataagg gagaagatgg tcaaggagga
     1861 ccaggatgca ctttcattct ggcttaagga caggaaggaa gggcttgtcc tccttgaagg
     1921 ggaggccttt gaggaaaaca gacccatata tacccatgcc agcataagta cttgggcctt
     1981 ccttaagtgg acaaaggacc atggtcttgg ggaagccagg agaaggcaaa aggagtacaa
     2041 aaatactctt ctgatttgat cagaagagta tttttatgga aatcattcct ccacaactat
     2101 atcgtaggtt acaggagccg ccttctgaag catggccgta actccgcagt acttttcctg
     2161 tgagagcttt acggcctttt ccagcttatc aaggggaagg tccttaccct taaaggtaaa
     2221 tgtaagatgt atatctgtgt ataccttggg gtggtcctca gcagcttccg cctctgcaga
     2281 tattttcata tcctggaggt caaccttcat tttatccaga atcattttta catcaactcc
     2341 agtgcatcct atgagtcctg caagaagcag ggccttgggt ctggggccct tgtcatttcc
     2401 tcccgcttca atggctgcgt ccgtaacgaa atgatggccg ttgatatcca tttcccaacc
     2461 catgtttccg gttatgttag catccagtct tgtctttgcc aaattgatca tctccttata
     2521 ctaagtcaat gggataatac ccaaaactcc taggcaataa tcctttcagc aagctttgcc
     2581 ttgtaaatat ccagaagctc cttctcggaa acatcacaac tgggcttgtc cagtattatt
     2641 tttcccttgt ccaacataat gatcctctga gagtatttca aggcatctcc catatggtgt
     2701 gaaaccatca tagtggttat attgtacttg tccagaagct cacgggtctt ttccatgacc
     2761 acatgggagg ttttcgggtc aagggctgcc gtatgctcgt caagtaggag aaggtcggga
     2821 tgcttcattg ccgccataat gagggaaagg ctttgtcgct gacctccgga aaggtatttc
     2881 acctgggtat ggagcttgtc ctccagccca aggtcaagat ccctaagcat ggaaacaaac
     2941 atctccagat tttttttctt gaccaggctc ctaagggaaa agctttcccc cttcttgttg
     3001 gccagggaca ggttttcaag gatggtaagg ctcggggata ctcccattga ggggctttga
     3061 tggactctac ccaggttcag tgcccttttt tcctcaggca gggaagtgat gtcctcaccg
     3121 ttgaggatta tctgtccctg gtcaacggga aggcttccac ttaccagatt cagaagagta
     3181 ctttttccac atccgtttgg cccgattatt gctgtgcact tgtttacttc aatatccaga
     3241 ttaaagcccc tgaagacgtc aatctgaaaa tcagtatctg aattaaagct cttccaaagg
     3301 tttcttactt ttaacacgga ccctacctcc ttgctttttt attgatttta taaatggact
     3361 tgaaaaatta ttgtagctta taaacacaat cactattatg gcacttattg cccta
//
LOCUS       DASRQF010000253         2316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1740469, whole genome shotgun sequence.
ACCESSION   DASRQF010000253 DASRQF010000000
VERSION     DASRQF010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740469"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /locus_tag="VFD79_06705"
     CDS             <1..1210
                     /locus_tag="VFD79_06705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HZH93775.1"
                     /translation="RDDMVTFLEEIIEHGNEVLKRTPEMLPVLKQAGVVDAGGKGLLY
                     LLEGALEAISDEKRSLVAPEKSQVVEVEVKKRKREHIDTDDIEFGYCTEFMINTNYGD
                     VEEFREQIVANGDSLLVVGGEGLIKVHIHTNNPGDVLEKALKLGELSDIKIDNMRYQH
                     EEVLLKDELLRLNDTEEKMESQEEKKYSIIAISIGEGIDELFKDMNVDIIVSGGQTMN
                     PSTEDILSAVDRTKGEHVIILPNNSNIILAAEQTKHLSDRSISVVPTKSIPQGIAALL
                     VLDVDEDLESNLENMSAAIGEVKTGQVTFAVRDTEFNGTDIRKNDIIGLSDKDILAAG
                     NDINKVSLQLVEKLVDEDSSIITIMYGSDTSLEQANDLADVLGEKFSDLDIEVVFGGQ
                     PLYYYLFAIE"
     gene            1330..>2316
                     /locus_tag="VFD79_06710"
     CDS             1330..>2316
                     /locus_tag="VFD79_06710"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZH93776.1"
                     /translation="MSKLSTSVQYIKGVGPKKAYRLKRLGLESVEDVLYYLPKDYEDR
                     RNISLLASGKYNEKGLYRVTVSSNPLVTRPRRGLTILKVQVRDSSKEAQLIWFNQDYL
                     REKLIPGREYIVYGKLKRDSYGLQIQNPEFIPWDGKSLNQGITPIYGLTDGITNKDML
                     KITKFALDNYLENVENLLPERISLKYGFSKKSDAIFQLHFPDSPESMIKARNQLAFEE
                     LLLLQLGLLRLNRESSQNTLSIRMPRHRLLESFIDQLEFRLTGAQNRVLEEILKDMGA
                     NKQMNRLVQGDVGSGKTIVGIIAMYNAVLNGYQATMMAPTEILATQHYETLVD"
BASE COUNT          753 a          385 c          567 g          611 t
ORIGIN      
        1 cagagatgat atggtaacct ttcttgaaga aatcatagaa catggtaatg aagtcttaaa
       61 gagaactccg gagatgctac cggtactaaa gcaggcggga gtggttgatg caggcggtaa
      121 aggcttgttg tatttgcttg agggagctct tgaggcaatt tctgacgaaa aaagatctct
      181 ggtcgcaccg gaaaaatccc aggtggtaga ggttgaagtc aagaaaagga agcgggaaca
      241 catagatact gatgatattg aattcggtta ttgtactgaa tttatgataa atacaaacta
      301 tggagacgtt gaagaattta gggaacagat tgttgcaaat ggggattcct tgctggtagt
      361 tggtggtgaa ggtctaatca aggttcatat ccataccaac aatcctggtg atgttcttga
      421 aaaggcattg aagcttggtg agctcagcga tattaagatt gacaatatga ggtatcagca
      481 tgaagaggtc ctcttaaagg acgagctatt gagattgaat gacacagagg agaagatgga
      541 gagtcaggaa gagaagaagt attccatcat tgccatatca ataggtgaag ggatagacga
      601 actttttaaa gacatgaatg ttgatataat agtttccggc gggcagacaa tgaatcccag
      661 tacggaggat atattatcag cagtggacag gactaaggga gaacatgtga taatactccc
      721 aaacaacagc aatattatat tggcggcaga gcaaacaaag cacctaagtg accgtagtat
      781 cagtgtggtc ccaacaaaat caatcccaca gggtattgca gctttactgg ttctggatgt
      841 ggatgaagac ctggaatcca atctggaaaa catgtctgca gcaatcgggg aagtaaaaac
      901 cgggcaggta acatttgcag taagggacac tgaattcaat ggaacagata tcaggaaaaa
      961 tgatatcatt gggctttctg acaaggatat acttgctgca ggtaatgaca taaacaaggt
     1021 ctccctgcaa ctggttgaaa aattggttga tgaggattcc tcaataatta ccataatgta
     1081 cggaagtgat acaagtttgg aacaagcaaa tgaccttgct gatgttcttg gagaaaaatt
     1141 tagtgacctg gatattgagg ttgtattcgg tggacagcca ctttactact atctatttgc
     1201 cattgaataa ccctgccatg tggcagggct tcttttttga tgctaacggg gaatttcaac
     1261 ttttttgatc atggatttcc ctacatgagt atcttaattg gtaataatga agctatcggg
     1321 ggtgtatttg tgtccaagtt aagtacttca gttcaataca taaagggtgt aggtcccaaa
     1381 aaagcataca ggcttaaaag actagggttg gaaagcgttg aggatgtact ttattatctg
     1441 cccaaggact atgaggacag aagaaatatt tccttgttgg cttccggcaa gtataacgaa
     1501 aaaggccttt acagggtgac agtaagctct aatcctcttg taacacgacc aaggagaggt
     1561 ttgacaatac ttaaggtcca ggttagggat agctcaaagg aagctcaact tatatggttt
     1621 aaccaggact acctgcggga aaagctgatt cctggtaggg aatatattgt atatggcaag
     1681 ctaaaaaggg attcctacgg gcttcaaatt caaaatcctg agtttatccc ttgggatgga
     1741 aagagtctaa accaggggat cacacctata tatggcctta cagatggaat cacaaacaag
     1801 gatatgttga aaatcaccaa atttgctctt gataattatt tggaaaatgt tgaaaacctg
     1861 ctaccagaga gaatttcttt gaaatatgga ttttcaaaga aaagtgatgc aatttttcag
     1921 ctccactttc cagatagtcc ggaatcaatg atcaaggcaa ggaaccaact tgcttttgaa
     1981 gaattattgc tattgcagct gggcctattg agattgaata gggagagctc ccagaacaca
     2041 ctcagtatca ggatgccaag acatagactt ttggaatcct ttatagatca actggagttt
     2101 aggctaacag gcgctcaaaa tagggtcctg gaggaaattc taaaagatat gggtgcaaac
     2161 aaacaaatga acaggcttgt ccaaggggat gttgggtcag gtaaaacaat tgtggggata
     2221 attgccatgt ataatgcggt tttaaacggc taccaggcta ctatgatggc acccacggag
     2281 attttggcca cacagcatta cgaaaccctt gtggat
//
LOCUS       DASRQF010000254         2912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_314823, whole genome shotgun sequence.
ACCESSION   DASRQF010000254 DASRQF010000000
VERSION     DASRQF010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314823"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..85
                     /gene="rrf"
                     /locus_tag="VFD79_06715"
     rRNA            <1..85
                     /gene="rrf"
                     /locus_tag="VFD79_06715"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            237..1496
                     /locus_tag="VFD79_06720"
     CDS             237..1496
                     /locus_tag="VFD79_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HZH93777.1"
                     /translation="MTDNVMKGSVSAPASMLAGAKKSVLALQHLIAMFGATVLVPMIT
                     GLNPSIALLSAGIGTLIFHTCTKGKVPVFLGSSFAFIPVILTVKELYNGDLAYAQGGI
                     VVAGLIYVLMSFLIKSIGVERIKKFLAPQVVGPMIIVIGLNLVPTAYGMASGNLMVAG
                     ITLGVALGINFLGKGFIKQLSILLAVMTGYAVAYSMGLVDTSIVGNASLVAVPNFTFP
                     KFDMGAIAIIAPVILAVFMEHLGDITTNGQVVGKDFIKEPGLNRTLLGDGLATTFAAL
                     IGGPANTTYGENTGVLAITKNYDPSILRLAAMFAVVLAFVAKIGAVLSSIPTSVMGGI
                     SLMLFSMIALIGVKTIKNNEVIFNWKNVVVMGTIIVLGLFSTQLSAMTGMTVGIPINE
                     TVSISGLSFAALVGIILNAALNIRKEA"
     gene            1577..2815
                     /locus_tag="VFD79_06725"
     CDS             1577..2815
                     /locus_tag="VFD79_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZH93778.1"
                     /translation="MNIKVAIIGGGASGMMAAINCAYTGAQVVLLERNDRVGKKLLAT
                     GNGRCNYTNENMRMDFLHGEDHQFIEMVMEKFGRDQTLDFFEKLGITPANEGDGKIFP
                     LSFQSSSMLDVLRYEMERTGVEVLTDSYVVEITKKKNFIIRLKTGEAIEAHRVVLTTG
                     GKAMPVSGSDGNGYSLVKPLGHGTNNITPALVQLKLEGDKFKQINGVKFVGRVSLHID
                     GLPIKEDFGDVLFTDYGISGPPVLQISREAIRAQEAGRDTRVKVSIISEKSKDEVFSY
                     LRERFENMPERALEFGLIGFINKRLILPIIKELNLDKYKAVGKLENREISALADLLTG
                     WEFQVTGNKGWGQAQTTAGGIITKDIDPDTMESRLVKGLFLAGEVLDVDGDCGGYNLQ
                     WAWSSRYLAGLSAGAMPRAD"
BASE COUNT          898 a          575 c          734 g          705 t
ORIGIN      
        1 acccataccg aacacagaag ttaagcctct taacgccgat ggtacttggt tggtaacgac
       61 ctgggagagt aggaagtcgc cagtctatat atatagcaaa ggctctgttg attattcaac
      121 agggtctttt tttcaaaaaa atatcttgaa tttcatctta accgatgata tactctttag
      181 aagtaagaaa aaaatacttt aaatcagtcc cgagaggctg gaaaggagag aataatatga
      241 ctgataatgt aatgaaaggt tcagtttctg cgcctgcgtc aatgcttgca ggagcaaaaa
      301 aatcggtttt ggcattacaa catcttatcg caatgttcgg tgcaacggtt cttgtaccaa
      361 tgatcactgg cttgaaccca tcaatagcac tactatccgc tggaataggt acactgattt
      421 tccacacctg cacaaaaggc aaagtacccg tattcctagg ctcatcattc gcttttatcc
      481 cggtaatact tacagttaaa gagttatata atggagatct ggcctatgcc caagggggta
      541 ttgttgtagc cggattaatt tatgtcctca tgtccttcct gataaagtca attggagtgg
      601 aaagaatcaa gaaattcctt gcaccgcagg tagtgggacc aatgataatt gtaatcggac
      661 ttaacctggt acccacagca tatggaatgg cgagcggaaa cctaatggta gcaggcataa
      721 cccttggagt tgccctggga atcaacttcc ttggcaaagg cttcatcaag cagctatcaa
      781 tactacttgc agttatgaca ggctatgcag tggcctactc aatgggactt gttgacacat
      841 caatagtagg gaatgcaagc ctagtggcgg ttccaaactt cacattcccc aagtttgaca
      901 tgggtgcaat tgcaataata gcaccggtta tactggcagt attcatggag caccttggtg
      961 acataacaac aaacggacag gtagttggaa aggacttcat caaagagcct ggacttaaca
     1021 gaacactgct tggagatgga cttgcaacga catttgcagc actaatcggt ggacctgcaa
     1081 atacaaccta cggagagaac actggagttc tggcaataac aaagaactac gatccctcaa
     1141 tactgagact tgcggcaatg tttgcggtag tcttggcatt cgtagcaaaa atcggagcag
     1201 tacttagctc aataccaaca tcagtaatgg gtggaataag cctgatgctc ttctccatga
     1261 ttgcactgat aggagttaag acaatcaaga acaacgaggt tatattcaac tggaagaatg
     1321 tagtggtcat gggaacaata attgtacttg gactcttcag cacacagctt tcagccatga
     1381 ctggaatgac agttggaata ccaattaatg aaaccgtgag cataagtgga ctaagctttg
     1441 cggcacttgt tggaataatc cttaacgcag cattgaacat tagaaaagaa gcataataca
     1501 agagaaaatt caagaataat ggggactatg gtataatata tcatagtctt taattttttt
     1561 tggaagaagg gataagatga acattaaggt tgcaataatc ggagggggag cctccggtat
     1621 gatggctgca ataaactgtg cctacacagg tgcccaggtt gtactcctgg aaaggaacga
     1681 cagggtgggc aagaagcttc ttgcaacagg caacggtaga tgcaactaca caaatgagaa
     1741 tatgaggatg gattttctcc atggagagga ccaccagttt atagagatgg tgatggaaaa
     1801 gtttggcagg gaccagacac tggacttttt cgagaagctt ggaatcacac cggcaaacga
     1861 gggagacggt aagattttcc ccctttcctt ccagagttcg tccatgcttg atgtcctgag
     1921 atacgagatg gaaaggacgg gggttgaagt ccttactgat tcatatgtgg ttgagataac
     1981 aaaaaagaag aactttatca taaggctgaa aacgggagag gccatagaag cccacagggt
     2041 ggtcttgaca accggaggca aggcaatgcc tgtaagcggg tcagacggca acggctacag
     2101 cctcgtgaag cctctggggc atggtacaaa caatatcacg ccagcccttg ttcagcttaa
     2161 gctggaaggg gacaagttca agcagataaa cggtgtaaag ttcgtaggaa gggtatccct
     2221 ccatatcgat ggacttccga ttaaggaaga ctttggggat gttctcttca cagactatgg
     2281 aatatcggga cctcccgtac ttcagatcag cagggaggcc attcgggccc aggaggctgg
     2341 aagggacact agggtaaagg tctcaatcat cagtgaaaag tccaaggatg aggtattttc
     2401 atacctgagg gagaggtttg aaaacatgcc tgaaagggcc ctggagtttg ggctcattgg
     2461 atttatcaac aaaaggctga tcctcccaat catcaaggag ttaaaccttg acaagtacaa
     2521 ggccgttgga aaacttgaaa acagggagat tagtgccctg gcagacctgc ttaccggctg
     2581 ggaattccag gttaccggca ataagggctg gggacaggcc cagacaacag ccggggggat
     2641 aataacaaaa gacatcgacc ctgacaccat ggaatcaagg ctagtaaagg gactctttct
     2701 tgcaggagag gttctggatg ttgatgggga ctgtggaggc tacaatctac agtgggcctg
     2761 gtcaagcaga taccttgccg gactttctgc aggggcaatg cccagggctg actaaactta
     2821 ttgtgtatca gaacattaaa tgtgttaaaa tattcatggc gcaagtgaaa tgaaaactaa
     2881 gtagtgcagg aggataagat gtttggatta ag
//
LOCUS       DASRQF010000255         5153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1649807, whole genome shotgun sequence.
ACCESSION   DASRQF010000255 DASRQF010000000
VERSION     DASRQF010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649807"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VFD79_06730"
     CDS             <1..268
                     /locus_tag="VFD79_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HZH93779.1"
                     /translation="HGIYVDIDKIVQREDKELIDVFANVICEKNSHKGMIIGKNGKML
                     KTIGQQAREDIEKLMGSKVNLQLWVKVEKNWREKANKVKYFGYK"
     gene            280..1014
                     /gene="recO"
                     /locus_tag="VFD79_06735"
     CDS             280..1014
                     /gene="recO"
                     /locus_tag="VFD79_06735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HZH93780.1"
                     /translation="MSETNGIVISGMKYKESSKILTLYTRELGKISVMAQGALKPKSG
                     IIASTEVFALSNWKLKKGRSFHYIESVDLVDSFYTIRDSMDKMLFGFYALELLDRSTP
                     MEEENKVLFELLEKFIRTMRKEERVLPMMVAYELKFASFIGYRPHLGGCVACGAKDSD
                     IWDFDHTKGGLICSNCRGGKGDPLLDGEVKQMKLLLMSKLEDVGDLQIPDKTLWKMHY
                     RMSDYLLYNLDIREMKSLSMIKQGLI"
     gene            1298..2185
                     /gene="glyQ"
                     /locus_tag="VFD79_06740"
     CDS             1298..2185
                     /gene="glyQ"
                     /locus_tag="VFD79_06740"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019106990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HZH93781.1"
                     /translation="MYFQDLMLKLLNYWGEKDCIIMEPYDIEKGAGTMSPHTFLRALG
                     PEPWQVVYVEPSRRPADARYGENPNRVYQHHQLQVILKPSPEDIQDLYLDSLKAIGID
                     PIKHDIRFVEDNWEAPTLGAWGLGWEVWLDGMEITQFTYFQQVGGINCDLESGELTYG
                     LERIAMYLQDVDNVFDIQWNKNFTYGDIFRKAEYEHSVYSFEKADIPVLKELFNTYEN
                     EANKVIAEELVIPSYDYVLKCSHTFNVLDARGAISVSERTNYIGRVRNLARSVASLYL
                     EQRKQMNYPLLKKGGGDNE"
     gene            2178..4256
                     /gene="glyS"
                     /locus_tag="VFD79_06745"
     CDS             2178..4256
                     /gene="glyS"
                     /locus_tag="VFD79_06745"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HZH93782.1"
                     /translation="MSREFLLEIGVEELPARYIKDAVEQLKGNMEKLLKEERLGYETF
                     HTYSTPRRLTIIVKGLGDEGESQEDLVKGPAARIAFDQDGKPTKALQGFMRGQGVTEE
                     NIKTMDYNGDGYIYAAVSKSGKPLQKVLEEGLEEVIKGITFPKSMRWGGKNLRFARPI
                     RWLVAILDNDILPFELEGIKAGNITRGHRFLGSQNIRIEGIDSYEKQLRDNFVIVDQE
                     ERKDIIRLKAEKLAKEKGGSIQKDENLLDEVTNLVEFPTPIIGRIKEEYLGLPFDVIT
                     TPMKEHLRYFPVLDDKGRLLPYFVTVRNGDSQYEETVVKGNEKVLGARLEDAKFFYKD
                     DVSIPLEGYVEKLKTLTFQEKLGTVHDKTNRVMKLTEKIGNYLEVGDETEKNLQRASF
                     LAKADLVTKMVTEFTELQGKIGMEYAQESGENEIVSLAIHEQYMPRFSGDELPTTTAG
                     AILSIADKLDSICGLFAIGIKPTGSQDPFALRRAAIGIINIIMDKKLNISLLELVDFS
                     LYIYVEENGLACDYNKVRSEIKEFFMGRVRNLFLDFGMAYDVIDSVINSGSDNIFDMR
                     IRVEKLSRWFENEDVGEILVAFNRLSSMATKTESTEVKRDLLTPDEIGLYDDYNNIEE
                     KVESHINKKEYDKAMILLGTLKDSINDYFDKVMVMVEDKELKENRLAMLRRIYSKMMK
                     VCDLTYIVAR"
     gene            4295..4897
                     /locus_tag="VFD79_06750"
     CDS             4295..4897
                     /locus_tag="VFD79_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZH93783.1"
                     /translation="MIDIVNENQPITSTAIAKKLGLTRATIRPDLSILTMTGILDARP
                     KVGYIYLGRPDRDLVADRISSIRVRDIMSLPVVIEETRTVYDAVVYMFTEDVGSIYIT
                     HSGYLSGVISRKDLLRSAVGGMDLNKTPVGMIMTRMPKLVVIRETDTILKAAIKLIEH
                     EIDSLPVVDSTGEEDKDLKVIGRITKTNITRMFVELGTNI"
     gene            4910..>5153
                     /locus_tag="VFD79_06755"
     CDS             4910..>5153
                     /locus_tag="VFD79_06755"
                     /inference="COORDINATES: protein motif:HMM:NF015575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HZH93784.1"
                     /translation="MSNRLTVYVLSDSIGETGELIARAAAKQFITENYEIKKYPFMDD
                     RDEIRNIFKEAVPGESLFIYTTVLKDIKDLIDSLGEE"
BASE COUNT         1677 a          920 c         1324 g         1232 t
ORIGIN      
        1 ccacggaata tatgtggata ttgacaagat tgttcagagg gaagacaagg aactgattga
       61 tgtttttgcc aatgttatct gtgaaaagaa ttcgcacaag ggaatgataa taggcaagaa
      121 cggcaaaatg cttaagacca tcggtcagca ggcaagagag gacattgaga agctgatggg
      181 tagcaaggta aaccttcaac tgtgggtcaa ggttgagaag aattggagag aaaaagcaaa
      241 caaggtgaag tattttggct acaaatagaa aggtctaaca tgtcagagac aaatggaata
      301 gtcatatcag gaatgaaata taaggagtcc agcaagattc taaccctgta cacaagggaa
      361 ctggggaaga tttctgtcat ggctcagggt gcattaaaac ccaaatcagg gattattgcc
      421 tctacggaag tctttgccct aagcaattgg aagctaaaaa agggtaggag ctttcattac
      481 atcgagagtg ttgaccttgt ggattccttc tataccatcc gggactcaat ggacaagatg
      541 ctttttggtt tctatgccct ggaacttctt gacaggtcca ctccaatgga ggaggagaac
      601 aaggttctgt ttgaactctt ggagaagttt atcaggacca tgaggaagga ggaaagggtt
      661 ctacctatga tggttgccta tgagcttaag tttgcaagct ttatcggcta cagaccccat
      721 ttgggtggat gtgttgcatg tggtgccaaa gactcggaca tatgggactt tgaccacacc
      781 aagggaggct tgatatgctc caactgccga ggcgggaagg gggaccccct gttggatggg
      841 gaggttaaac agatgaagct cctcctgatg tccaaactgg aagatgtggg agatcttcag
      901 atccccgaca agaccctgtg gaaaatgcat tacaggatgt cagactacct tttgtataat
      961 cttgatatta gggagatgaa gtcattgtcc atgataaaac aaggcctaat ctaggctgag
     1021 ctaccggact cttgacataa tgacgaaatt ttgttaaatt aataaacgaa actgaattcc
     1081 atacgatgat gaagaaaata cctgtaactg tgtagcaaag agagtccggc aagggtggaa
     1141 gcggatgtat acagtcattg ggaacggcgc ttctgagtat tgcagtttaa gagagtacct
     1201 ttcacaggta ttaaataggg tggaaccgcg gaagagacct ttcgtcccta tagggacgct
     1261 gaggtttttt tgtatccaaa aataaagggg ggttattatg tattttcagg atttgatgct
     1321 gaaattactg aattactggg gtgaaaaaga ttgtataata atggagccat atgacatcga
     1381 aaagggtgct ggaacaatga gtccacacac cttcttaagg gcattggggc cagagccatg
     1441 gcaggtggtt tatgtggagc catcaagaag acctgcagat gcacgctacg gcgagaatcc
     1501 aaacagggtt taccagcatc atcaacttca ggttattcta aagccttcac ctgaagatat
     1561 tcaggacctg tatctggaca gcttgaaagc aataggcatt gacccgatta agcatgacat
     1621 aagatttgta gaggacaact gggaagctcc aactcttggt gcatggggac ttggttggga
     1681 agtatggttg gatggaatgg aaataacaca atttacatac tttcaacagg taggaggaat
     1741 aaattgtgat ttggagtctg gagagctgac ctacggactt gagaggatag caatgtatct
     1801 tcaggatgtt gacaatgtct ttgacattca gtggaacaag aacttcactt atggagacat
     1861 atttagaaag gctgaatacg agcactctgt atacagcttt gaaaaggcag acattccagt
     1921 gcttaaggaa ctcttcaaca catatgagaa cgaagccaac aaggtgattg cagaagaact
     1981 ggtcatacct tcatatgatt atgtactgaa atgctcccat acattcaatg tgctggatgc
     2041 aagaggggcc atctctgttt ccgagagaac aaactatatt ggcagggtca gaaacctggc
     2101 aagaagtgtg gcatctcttt atctggagca gaggaaacag atgaattatc ccttattgaa
     2161 aaaaggtggt ggagacaatg agtagagaat tcctgcttga aattggagtt gaagaactac
     2221 cggcaaggta tataaaagat gcagtggaac agttaaaggg taacatggag aaactgttaa
     2281 aggaagagag actaggttat gagaccttcc atacctattc aacaccccgt agacttacaa
     2341 taattgtgaa aggacttggg gatgaagggg aaagtcaaga ggaccttgtc aagggaccag
     2401 ccgcaagaat agcttttgat caggatggca agccaacaaa ggcattgcag ggctttatgc
     2461 gcggccaggg agttacagag gaaaacatca aaaccatgga ctacaatggc gatggatata
     2521 tatacgcagc agtaagcaaa agtggaaaac cccttcaaaa agttcttgaa gaaggtcttg
     2581 aagaggtgat aaagggtata accttcccta aatccatgag atggggaggc aagaacctaa
     2641 ggtttgcaag acccataagg tggctggttg caattttgga taatgatatc ctgccatttg
     2701 agctggaggg aataaaggca gggaacataa cgaggggaca tcggttcctg ggtagtcaga
     2761 atatccggat tgaaggtata gacagctatg aaaagcagct tagggacaac tttgtaatag
     2821 ttgaccagga ggaaagaaag gacataataa gactcaaggc tgagaagctt gcaaaggaaa
     2881 agggtggatc catccagaag gatgagaacc ttctggatga ggtaaccaac cttgtggaat
     2941 tcccgacccc aataattgga aggataaagg aagagtacct gggacttcct ttcgatgtaa
     3001 taaccacccc catgaaggag catttgagat actttcctgt ccttgatgac aagggaaggc
     3061 ttcttccata ctttgtaact gttagaaacg gtgactcaca atacgaggaa acagttgtaa
     3121 agggaaatga aaaggttctt ggggcaaggc ttgaggatgc caagttcttc tataaggacg
     3181 atgtcagcat tcctctggag ggatatgttg agaagctcaa gacattgacc ttccaggaaa
     3241 agcttggaac agtacatgat aaaaccaaca gggtgatgaa gctaaccgaa aagatcggca
     3301 actatcttga agtgggagat gagacagaga agaacctgca aagggcaagc ttcctggcaa
     3361 aggcagacct ggttacaaag atggtaactg aatttacgga actccaggga aagatcggaa
     3421 tggaatacgc tcaggaatca ggagagaatg aaattgtaag cctggcaatc cacgagcaat
     3481 acatgccaag attttctggc gatgagcttc caacaaccac tgccggtgca attttatcaa
     3541 tcgcagacaa gcttgattca atttgtggac tctttgcaat tggaatcaag cccaccgggt
     3601 cccaggaccc ctttgcccta agaagggctg cgataggtat aataaacatc atcatggaca
     3661 agaagcttaa catttccctt ctggagctgg ttgacttttc cctgtacatc tatgttgagg
     3721 agaatggtct tgcctgtgac tacaacaagg taaggtctga aatcaaggaa ttcttcatgg
     3781 gtagggtaag aaacctcttc ctggattttg ggatggcata cgatgtcata gattctgtaa
     3841 tcaactcagg tagtgacaat atttttgaca tgaggataag agttgagaag ttgtccagat
     3901 ggtttgaaaa tgaagatgtg ggagagatac tggttgcctt caataggctt agttccatgg
     3961 caacaaagac cgaatccacc gaagtgaaaa gagacctcct gactccagat gagatagggc
     4021 tctatgacga ctacaacaac atagaggaaa aggttgaatc tcacattaac aagaaggaat
     4081 atgacaaggc aatgatactg cttggcaccc tgaaggactc cataaacgat tattttgaca
     4141 aggtcatggt aatggttgaa gacaaggagc ttaaggagaa cagactggcc atgcttagac
     4201 gaatatactc aaaaatgatg aaggtctgtg atctgacata cattgttgcc agataggtgg
     4261 tggtattatc caactttcaa acaggcagaa taaaataatt gatatagtca atgaaaacca
     4321 accaataaca agcacagcca ttgcaaaaaa actgggtctg accagggcga ccatcaggcc
     4381 tgacctgtca atccttacaa tgaccgggat actggatgca aggccaaagg tgggatatat
     4441 ctacctgggc agaccggaca gggatcttgt tgctgacagg atttcctcca taagggttag
     4501 ggatatcatg tccctgcctg ttgtgatcga agagaccaga acggtctatg atgcagtggt
     4561 ctatatgttc acggaggatg tgggatccat ctatatcacc cacagcggat acctctcagg
     4621 tgttatttca agaaaggacc ttcttagaag cgctgttggg ggaatggacc tgaacaagac
     4681 cccagtgggc atgattatga ccaggatgcc taaactggtt gtcataaggg agactgatac
     4741 aatcctaaag gctgccatca agttgattga acacgaaata gacagtttgc ctgtggtcga
     4801 ttcaactggt gaagaggata aggacctgaa agtcattgga agaataacca agacaaacat
     4861 aacgagaatg tttgttgaat tgggaacaaa tatctaggag ggggtcaata tgagtaatag
     4921 actaacagtc tacgtactat cagattccat tggtgaaact ggtgagctga tagcaagagc
     4981 agctgcaaag cagtttataa cggaaaatta tgaaataaag aaatacccct tcatggatga
     5041 cagggatgag ataagaaaca ttttcaagga agcggtccca ggagaaagcc tattcatcta
     5101 tacaactgtt ctgaaggata taaaagacct gattgacagt cttggagaag aaa
//
LOCUS       DASRQF010000256         9064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1279687, whole genome shotgun sequence.
ACCESSION   DASRQF010000256 DASRQF010000000
VERSION     DASRQF010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 9064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9064
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279687"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VFD79_06760"
     CDS             complement(<1..207)
                     /locus_tag="VFD79_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93785.1"
                     /translation="MKLASIISWMGVGAMSLALYHGFTSGDFFADGGMILNNPWGIVS
                     LVDLYVGFTISSIWIFFREKNIYIS"
     gene            332..1435
                     /locus_tag="VFD79_06765"
     CDS             332..1435
                     /locus_tag="VFD79_06765"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HZH93786.1"
                     /translation="MKIHDLDTPSLLIRQDIMLKNIVGMQKYADENNVSLRPHTKTHK
                     SSTLALVQADQGASGITVAKVGEAEVMANAGLEDIFIANEIVGEMKLNRIKALQKTAK
                     ISFGLDSIAQAKIIEKVFDKDEKARVLIEIEVGENRSGIIEEDDFIELINYIKTSKCI
                     DFKGIFSHDGHSYGADDIHELSEIHLESQRRTLRFADLAREAGLAPETVSIGSTPSLV
                     HNFPILDGVTEIRPGTYIFMDASQANVVGGLQMNAASVLATVMSKPTMERVVLDVGAK
                     GLTMQKRTKGLTATKGMGIILGFEGVEIDSMYDEHALIYDRDFRNQVQVGDRVQIIPN
                     HICPVVNLYDRIYLVSGEDVLQEIAIDCRGRLQ"
     gene            complement(1466..2914)
                     /locus_tag="VFD79_06770"
     CDS             complement(1466..2914)
                     /locus_tag="VFD79_06770"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006968.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HZH93787.1"
                     /translation="MENGVMLQGFEWYLPGDGSFYKMMVEKTEELANAGFSAVWIPPV
                     FKGMGTEDVGYGTYDLYDLGEFQQKGTIRTKYGTKQELLHMIERFHNKGVQVYADVVM
                     NHKAGGDRQEKFIAIQVDSHDRNKEIGKPVEISAWTGFDFSGRGGEYSSFKWNYNHFN
                     GVDYDDLTGKKGIFRIIGENKGWNLGVSVERGNYDYLMFTDIDHAHPEVKEEFKKWAK
                     WFIQETGIDGFRLDAVKHIDMAFLREFVNEVREYAGQDFYVLGEYWVDNMDWIEEFLE
                     GTSFGIDVFDVPLHFRLHRASIEGDKYDLRTILDETLVSRYPTEAITFVDNHDSQPGQ
                     SLQSWVEPWFKEIAYSLILLRSKGYPCVFYGDYYGMGGEYPYEGMKESINNLIHLRQN
                     YCFGEEDDYFQNPNLIGWVRKGTKENPQKTAVVISNKEGGKIRMFMGHELIGREFLDK
                     LERSRDTVVIDDEGFGEFSTPQAGVSAWVLKE"
     gene            complement(2931..3827)
                     /locus_tag="VFD79_06775"
     CDS             complement(2931..3827)
                     /locus_tag="VFD79_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZH93788.1"
                     /translation="MFTQKRLDQAYEGARLEYFDDSSKYVFMSDHHRGDGGTSDEFTK
                     NQNVFLFALDHYYKNGFTYVEVGDGDELWEQPKFKYIKNAHYDVFENIKKFFYHDRLI
                     MLYGNHNIYLKDHDYVKKYYYSYYNDFREQRFDFLKGLVPIESLVLKHRKTGQEILVV
                     HGHQGDLANDQLWFPTMLSLKYFWRFMHSFGIRNPASPTKNVYKRHKIEKNFNKWILK
                     NRKMVICGHTHRFKFPRTDDLPYFNTGCCIYPTTITAIEMEGGRIQIVRWKMRVNQDG
                     LLQAKREVIRGSELIGKYDIRE"
     gene            complement(3858..4544)
                     /locus_tag="VFD79_06780"
     CDS             complement(3858..4544)
                     /locus_tag="VFD79_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZH93789.1"
                     /translation="MADIVRLEEIDKFYGEKIKTQVLFDVNLTFEENSFNSIIGQSGS
                     GKSTLLNIVGTLDKPTNGRVYINDRRTDTMNPMQLADLRNRTMGFIFQFHYLLPEFTA
                     KENVLMPSRIMNYQVTTEINQWADELMDLTGLTKVKNNMANAMSGGQQQRTAIARALI
                     NKPSIVLADEPTGNLDSDTSESIYGLLRDINKEYGTTFIIITHDQRIAEKTDRIVEIK
                     DGRINLDVKR"
     gene            complement(4537..5697)
                     /locus_tag="VFD79_06785"
     CDS             complement(4537..5697)
                     /locus_tag="VFD79_06785"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZH93790.1"
                     /translation="MRLAFKIALRFLKSGKGQTILIVLGISIGVAVQLFIGSLIQGLQ
                     INLVNTTIGSSSQVTITSTSEDKLIDNWDRTLYEASLFSPDVINVSVAADGPATLDYE
                     TDTAPVLIRGFNIQDSNEIYKIQEGIYAGRLPERKDEVAIGRELSLLAGIETGDRILV
                     LNPGGGSARFDVTGLYDLGVSSINESWIITNLQTAQDFLGMGDKVSSVEMQVSEVFEA
                     DIIGERIVYALSDKSLRVDNWKDQNQSLLSGLNGQSVSSYMIQVFVLVAVLLGISSVL
                     AISVVQRSKQLGILKAMGIKDREASLIFVFQGLMLGILGAIMGVAFGFGLLYSFTKFA
                     VNPDGSPVVPIYINYGFIALSGMFAVASAMIASLIPARISSRLNPIEVIKNG"
     gene            complement(5694..6344)
                     /locus_tag="VFD79_06790"
     CDS             complement(5694..6344)
                     /locus_tag="VFD79_06790"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HZH93791.1"
                     /translation="MNKYYEEFMELFSKNLKDRCVIWALEQVREGRLTIPELYSDVLV
                     PSLYSIDDCPEDSPGCIWDEHVKTSIIRTIIETLYPTVIKLAGESEKKGKKIVLTCPE
                     KEYHEIGLRIVADFFQINGYDVIFVGSNTPRAQIWEVIRSEKPDYVGISVTDHYLLSE
                     AKKIMERIKDECTKDVKILLGGQAFRKNPHTVEDFGADLYIETYEDVLSLGKEEDK"
     gene            complement(6355..7620)
                     /locus_tag="VFD79_06795"
     CDS             complement(6355..7620)
                     /locus_tag="VFD79_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZH93792.1"
                     /translation="MTKKKGFLLLGAIIVVAALIYFFGINGSSGIEVSTMEVVEGSIQ
                     ENLDITGKVISEDIQQIFVPAGTEVLQVMVKEGDMVSRGDTLAVLDPSELQTRVEKLR
                     INLEQVEADLRMTGGSADRQILQNNLQKAQESLSTAVRDYETSKAKLDDMRVLYENGA
                     ISLAEFENQENALKSLESSVKSASLNVEDARLRYSDFGTNSSSTAGSLERQKRSLLLD
                     IQQLEDQIDDTRIIADLDGTVVVLDLVENRTLGREAEVVVQDTSKFQFEALVTQEDAV
                     NIQRGQDATIKVSGLSNEYTGKVVEIGSKAEVDPSSGSSTPKVKISIELNEVDERLVS
                     GFDADAQITTGMVQDVLVIKNEAIREDDQLRSFVFKLENGIAVRTPVTRGITDRYLTE
                     IIEGLQLGDIIILNPPLELEDGRAVSVIE"
     gene            complement(7660..7959)
                     /locus_tag="VFD79_06800"
     CDS             complement(7660..7959)
                     /locus_tag="VFD79_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93793.1"
                     /translation="MIRVTRLGGKQTLAIGFVVIILTGALLLMLPVANKNGEFLPFLD
                     ALFTSTSATCVTGLVVYDTWSQFTLFGQVVFRFLQIKLLNILQKYLQNMVHNWFF"
     gene            complement(7956..8411)
                     /locus_tag="VFD79_06805"
     CDS             complement(7956..8411)
                     /locus_tag="VFD79_06805"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HZH93794.1"
                     /translation="MEVLAVGSIVALMILGYFLMDKIDMYFVKNTEAREEIPIDRRTI
                     LVYTDCESEEEICQVFEKMKFSYYLIDDSRIPENIEFKTFFALSEDDGENLFLSRMVR
                     KRSKNTWVVARINDYIYADIYRKIGVNKVITGDVTRDRIVEALQGGDLQ"
     gene            complement(8546..>9064)
                     /locus_tag="VFD79_06810"
     CDS             complement(8546..>9064)
                     /locus_tag="VFD79_06810"
                     /inference="COORDINATES: protein motif:HMM:NF020950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HZH93795.1"
                     /translation="NGMPPEDTVVFLDDEGIPPAELGYIYRLRALGIVQGDGGNFMPQ
                     KRLTRAEAAAMMNRVMMMLRSAPTEYFYDPNMLTVMLHENPTTGYGWDVQFKEGEILK
                     IESDEFVPFAVAPDVVGSGGERTWVFVPEGEGVEEITFSYYRSWEEKETAIDVRVLTV
                     EVDENIEITNVR"
BASE COUNT         2442 a         2245 c         1606 g         2771 t
ORIGIN      
        1 tgaaatatag atgttctttt cccggaagaa tatccatatg gaagatattg taaatccaac
       61 atacagatca accaaggaga ctatacccca tggattattc agtatcatcc ctccatctgc
      121 aaaaaaatct cctgacgtaa agccgtgata cagtgccaag gacattgctc caacacccat
      181 ccatgatata atgctcgcca atttcatatg cactcctcca atctgtatag ttgtatttta
      241 ccctgattaa ccacctttaa ctcccttgta gatattatct tcggcattgg gtatatagga
      301 cttagtattt ataattaaaa gggggattac tgtgaaaatt catgatcttg atacaccatc
      361 cctgctaata aggcaggaca ttatgcttaa aaacattgtt ggaatgcaaa aatatgccga
      421 tgagaacaat gtttcactaa ggcctcatac caagacccat aaatcatcca cccttgccct
      481 ggtccaggca gaccagggtg catctggaat aacggttgcc aaggttggcg aggcagaggt
      541 aatggccaat gcgggacttg aggatatatt catcgccaat gagattgtag gggaaatgaa
      601 gctaaataga ataaaagccc ttcaaaaaac ggccaaaatt tcctttggcc tggactccat
      661 agctcaggcc aagataatag aaaaggtgtt tgacaaggat gaaaaagccc gtgtccttat
      721 tgaaatcgag gttggggaaa acaggtccgg cataattgag gaggatgatt tcatcgagct
      781 aataaattat atcaaaacct ccaagtgcat agatttcaag ggtatattct cacatgacgg
      841 acattcctat ggggcagatg atatccatga gctaagtgaa attcaccttg aaagtcaaag
      901 aagaacctta agattcgcag atttggcaag ggaggctggg ctggctccag aaacagtcag
      961 tataggctca actccctcct tggtccataa ctttccgatt cttgatggag ttactgaaat
     1021 aagaccggga acctacattt ttatggatgc atcccaggca aacgtggttg gaggactcca
     1081 aatgaatgca gcctcagttc ttgcaactgt aatgagcaag ccaactatgg agagggttgt
     1141 tcttgatgtt ggcgccaagg gcctgacaat gcaaaaaagg accaagggat tgactgcaac
     1201 caagggcatg ggaataatat taggatttga aggagtggaa atagattcaa tgtatgatga
     1261 acacgccctg atctacgaca gggattttag aaaccaggtg caggtgggag acagggtcca
     1321 gattatacca aaccatatct gtcctgtggt gaatctctac gataggatat atctggtttc
     1381 aggcgaggat gtcctccagg agatagcaat cgactgcaga ggaagactgc aatgaaaaaa
     1441 cggctcgaaa aagccgtttt ccttattatt cctttaatac ccatgcacta acaccagctt
     1501 gtggagtgct gaattcccca aatccttcat catctatgac aacagtatcc ctgcttcttt
     1561 ccagcttatc caaaaattcc ctcccaatta attcatgccc catgaacatc cttattttgc
     1621 caccctcctt gttggaaatg acaacagcgg tcttttgggg attttcttta gtgcctttcc
     1681 gcacccatcc aatcaggttt ggattctgaa aatagtcatc ctcttcacca aagcaatagt
     1741 tttgtcttaa gtggatgaga ttattgatgc tctccttcat tccctcatac ggatattctc
     1801 cacccatacc gtaatagtct ccgtagaata cacatgggta gcccttgctc cgcaataata
     1861 tcaaggagta agcgatttcc ttgaaccaag gttcaaccca tgattggagt gactgtccag
     1921 gctgtgagtc atggttgtcc acaaaagtaa tggcttccgt tggatacctt gatacaagtg
     1981 tttcatcaag aattgtcctt aaatcgtact tgtccccctc tatagatgcc ctgtggagcc
     2041 taaaatgaag gggtacgtca aaaacatcta tcccaaatga tgtcccttca agaaactcct
     2101 ctatccagtc catgttgtcc acccaatact ctcccaaaac atagaagtcc tggcctgcat
     2161 attccctaac ctcatttaca aactccctta ggaaggccat gtctatatgt ttcaccgcat
     2221 caagtctaaa tccatcgatt ccggtttcct ggataaacca ttttgcccac ttcttgaatt
     2281 cctcctttac ctccgggtgt gcgtggtcga tatctgtaaa cataaggtaa tcataatttc
     2341 ccctttcaac agaaacccca agattccatc ccttgttctc tccaattatc ctaaaaatgc
     2401 ccttctttcc cgtaaggtca tcatagtcaa caccgttgaa gtgattgtaa ttccatttaa
     2461 aacttgaata ttcacctcct cttccagaaa aatcaaagcc tgtccaagcg cttatctcaa
     2521 ccggttttcc aatctccttg ttccgatcgt gagaatccac ctgaatggct ataaacttct
     2581 cctgcctatc tccacctgcc ttgtggttca tcacaacgtc tgcataaact tgcacaccct
     2641 tgttgtggaa cctttcaatc atatgtaaga gctcctgctt tgttccatat tttgtcctta
     2701 ttgtaccctt ttgctggaac tcacccaggt cataaaggtc gtatgtgcca taaccgacat
     2761 cctcagttcc catcccctta aaaacgggtg ggatccatac agcggaaaag cctgcatttg
     2821 caagctcttc agttttttca accatcatct tataaaagct tccatctcct ggaaggtacc
     2881 attcaaatcc ttgaagcatt actccgtttt ccataaccct tcctcccaac ctattccctt
     2941 atatcgtact taccaatcag ttcagacccc ctgataacct cccttttagc ctggagcaag
     3001 ccgtcctggt tcaccctcat cttccacctg acaatctgga tcctaccacc ctccatctcg
     3061 atggcagtta tggtggttgg gtagatgcag cagcctgtat tgaaataagg caaatcatcc
     3121 gttcttggga acttaaacct gtgtgtatgg ccgcatatga ccatctttct atttttcaat
     3181 atccatttat tgaaattctt ttcaatttta tgtctcttgt atacattctt ggtagggctg
     3241 gcagggttcc ttattccaaa ggaatgcata aacctccaaa aatactttag tgatagcatt
     3301 gttggaaacc acagctggtc gttggccagg tctccctgat gaccatggac cacaagtatt
     3361 tcctggccgg tttttctgtg tttaagaaca agggattcaa ttggcactag cccctttaga
     3421 aaatcaaatc tctgttcccg gaaatcgtta tagtaggaat agtaatactt cttgacgtaa
     3481 tcatggtcct taagatatat gttgtggttg ccataaagca taatcagcct atcatggtag
     3541 aagaacttct tgatattctc aaacacatcg taatgggcat tcttgatgta tttgaacttt
     3601 ggttgctccc aaagttcatc cccatccccc acttccacgt aggtgaagcc atttttgtag
     3661 tagtggtcca aggcaaatag gaatacattc tgattctttg tgaactcatc tgatgttcca
     3721 ccatctcctc tatggtggtc actcatgaac acatactttg agctatcgtc gaagtactct
     3781 agccgggcac cttcataagc ctgatcaagt cttttttgtg taaacataag cttcactccc
     3841 cacaagcatt gttatgccta tctctttaca tcaagattaa tccttccatc cttaatctcc
     3901 actatacggt cagtcttttc tgctatcctc tgatcatgtg taattatgat aaaggtggtt
     3961 ccatattcct tgtttatgtc ccttaaaagt ccatagatgc tttcagatgt atctgagtca
     4021 aggtttcccg ttggttcatc cgccagcact atggagggct tgtttataag ggcccttgca
     4081 atcgctgttc tttgctgctg gccacctgac attgcatttg ccatgttgtt cttgaccttg
     4141 gtaagacctg tcaaatccat cagttcgtct gcccactggt tgatttctgt ggtcacctgg
     4201 taattcatga ttctgcttgg cataaggacg ttctcctttg cagtgaactc tggaagaagg
     4261 taatggaact gaaaaatgaa tcccatggtc ctattcctaa ggtctgccag ctgcataggg
     4321 ttcatggtgt cagttctcct gtcgtttata tatacccttc cgtttgttgg tttgtccaag
     4381 gtcccgacga tattcagcaa ggtactcttt ccgcttcccg actgtccaat aattgaatta
     4441 aagctattct cttcaaaggt caggttcaca tcgaaaagta cctgggtctt gattttttct
     4501 ccgtaaaact tgtctatctc ttccaatctt actatatcag ccattcttga tcacctcgat
     4561 cgggttcagt ctggaggaaa ttcttgctgg tatcaatgat gctatcattg ctgatgccac
     4621 tgcaaacata cctgacaagg ctataaaccc gtagtttata tatattggaa caactgggct
     4681 tccgtctgga tttactgcaa atttggtgaa ggaataaagc aatccaaatc caaaggccac
     4741 tcccatgatt gctccaagta ttcccagcat cagaccctga aaaacaaata tcagactcgc
     4801 ttcacgatcc ttgattccca ttgccttcaa tattcccaat tgctttgacc tttgtacgac
     4861 ggatatggca agtacacttg atatacccag taaaactgcc accagaacaa atacctgtat
     4921 catgtagctt gatacactct gtccattaag tccgctcaga agactttggt tctggtcctt
     4981 ccagttatct acccttagac ttttatcaga cagggcataa actatccttt ctccaattat
     5041 atcagcttca aaaacttcag aaacctgcat ttcaacagaa gagaccttgt ctcccatacc
     5101 cagaaaatcc tgtgcggtct gaagatttgt tataatccat gattcgttaa tggaggatac
     5161 ccccagatca taaagcccgg tcacatcaaa gcgtgctgac cctcccccag gattcagaac
     5221 caggattctg tcacctgtct caattcctgc aagtagtgac aactccctgc ctattgcaac
     5281 ttcgtccttc ctttcaggca accttcctgc atagatccct tcctgtattt tgtatatttc
     5341 atttgagtcc tgaatattaa agccccttat aaggactggt gcagtgtcag tttcataatc
     5401 aagtgttgca gggccatcgg ccgctactga aacattgatt acatcgggac taaacagact
     5461 tgcttcatac agtgtcctgt cccaattgtc aataagcttg tcctcacttg tggaggttat
     5521 tgtaacctgt gatgagctgc cgattgttgt gttaaccaag tttatctgta gaccttgaat
     5581 cagagatcct ataaagagct ggacagcaac tccaattgag attcctaaaa caataaggat
     5641 ggtctgacct tttcctgatt taagaaatct tagtgcaatc ttaaaggcta gtctcatttg
     5701 tcctcctcct ttcccaggga aaggacatcc tcgtatgtct caatgtaaag atctgcccca
     5761 aaatcctcca ctgtgtgagg atttttcctg aaagcctggc ctcctagaag aatctttaca
     5821 tccttggtac actcatcctt gattctttcc ataatcttct tagcttcaga aagcagatag
     5881 tggtcagtta cactgattcc cacataatcc ggcttttcag accttattac ctcccagatt
     5941 tgagctctgg gtgtattgct gccaacaaag atgacatcgt atccattgat ttgaaaaaaa
     6001 tcagccacta ttctaagacc tatttcatga tattcttttt caggacaggt tagaacaatc
     6061 ttcttcccct ttttttcgct ttccccagca agcttaatca ctgtgggata aagtgtctca
     6121 attattgttc tgatgattga ggttttcaca tgctcatccc aaatgcaacc agggctgtcc
     6181 tctggacagt catcgatact gtatagagat ggaaccaaca catcactgta tagctcaggt
     6241 atagttaacc ttccttccct tacctgttcc agtgcccata ttacacacct atccttcaag
     6301 ttcttggaga aaagctccat aaattcttcg tagtatttat tcatgccctt cctcctattc
     6361 tataacgctt acagcccttc catcctccag ctcaagtggt gggtttagga ttattatatc
     6421 acccagctga aggccttcaa ttatttcagt caaatatctg tctgtaatgc ctcttgtgac
     6481 aggtgtcctc acggcaattc cattttcaag cttaaacaca aatgatctca actggtcatc
     6541 ttccctaatg gcttcattct taatcaccaa gacatcctgg accatcccgg ttgtgatctg
     6601 ggcatctgca tcaaaaccgg aaaccagtct ttcatccact tcattcaact caatggagat
     6661 tttaaccttt ggagtactgc ttccacttga agggtctacc tctgccttgc tgccaatttc
     6721 cactacctta ccagtatatt catttgaaag tccagacacc ttgatggttg catcctgacc
     6781 tcgttggatg ttcactgcat cttcctgggt tacaagggct tcaaactgga acttggaggt
     6841 atcctgaacc actacttcag cctccctgcc aagtgtcctg ttttctacca gatccaatac
     6901 cacaacagta ccatccaggt ctgcaattat cctggtatca tcaatttggt cttcaagctg
     6961 ctgaatgtcc aaaagcaatg atcgtttctg tctttcaaga cttcccgcag tactggatga
     7021 gtttgttcca aaatcagaat accttaatct tgcatcctct acattcaagc ttgctgactt
     7081 tacacttgac tcaaggctct taagggcatt ctcctgattt tcgaattcag ccaggcttat
     7141 agctccattc tcatacagaa ccctcatgtc atccagtttt gcctttgatg tttcatagtc
     7201 tctaactgca gtggagagtg actcctgtgc cttctgcaga ttattttgca gaatctgtct
     7261 gtctgcagac cctcctgtca ttctcaggtc tgcctccacc tgctcaaggt ttatccttag
     7321 cttctccact cttgtctgga gttctgatgg gtccagtact gccaaggtat cacccctact
     7381 gaccatatcc ccctccttga ccattacctg gaggacttcc gtgccagctg gtacaaatat
     7441 ttgctgaatg tcctctgata tcaccttacc ggttatgtca agattctctt gtatggaccc
     7501 ctccacaacc tccattgtac ttacctctat ccctgatgat ccattaattc caaaaaaata
     7561 aatcaatgca gctactacaa tgattgctcc caataataga aatccctttt tctttgtcat
     7621 gccctcactc ctcccaattt ctgtatgcta caattatact caaaaaaacc aattatgaac
     7681 catattttgt aaatattttt gtaatatgtt caatagtttt atttggagaa agcgaaagac
     7741 cacctgacca aaaagtgtga actgggacca tgtatcgtaa accacaagac ctgtaacgca
     7801 tgtggcagat gttgatgtga agagggcatc aaggaatggc agaaactcac cgttcttgtt
     7861 ggcaacagga agcatcagaa ggagggcccc tgttagtatt ataaccacaa agccaatggc
     7921 caaggtctgt tttcctccaa gcctggtcac cctaatcatt gaaggtcacc cccctgaaga
     7981 gcttcaacta tcctatcccg agttacatca cctgttatta ccttattaac tccaatcttt
     8041 ctgtatatgt ctgcatatat ataatcgttt atcctagcaa ccacccaagt attcttgctc
     8101 cttttcctta ccatcctgga caggaagaga ttctccccat catcctccga caaggcgaaa
     8161 aatgttttaa attcaatgtt ttcaggtatt cttgaatcat caattaaata gtatgaaaac
     8221 ttcattttct caaatacctg acatatctct tcctctgatt cacagtccgt atatacaagt
     8281 atcgtcctcc tatctatagg tatttcctct ctggcttccg tgttcttaac aaaatacatg
     8341 tctatcttat ccatcagaaa atatcccaga atcattagtg caactatgga cccgaccgct
     8401 aatacttcca tagataagac ctcccctcat ctaaatctta tctcagaaaa tattaagata
     8461 gtgttcgtat tgtctcttag tatgttattt ttgtgcaaag aaaaagctat tcccacttgg
     8521 agaatatctt atgtttaatt ctagtttacc taacattggt tatttctata ttttcatcca
     8581 cttctactgt cagtacccta acatcgattg ctgtttcttt ttcttcccag cttctgtaat
     8641 atgagaaggt tatttcctca acaccctcac cttcaggcac aaacacccag gttctttctc
     8701 cacctgagcc cacaacatcc ggtgccacag caaatggaac aaattcgtca gactcaattt
     8761 ttagaatttc accttcctta aactggacat cccagccgta accggttgta ggattctcat
     8821 gcagcattac agttaacatg tttggatcat aaaaatactc tgttggggct gaccgaagca
     8881 tcatcatgac cctgttcatc atggcagcag cctctgctct tgtaagtctt ttctggggca
     8941 tgaaattacc tccatcccct tggacaatcc ctaatgccct aagcctgtag atatagccaa
     9001 gttcagcagg tgggatgccc tcatcatcaa ggaataccac agtatcttca ggtggcatac
     9061 catt
//
LOCUS       DASRQF010000257         2277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_38497, whole genome shotgun sequence.
ACCESSION   DASRQF010000257 DASRQF010000000
VERSION     DASRQF010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38497"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VFD79_06815"
     CDS             complement(<1..421)
                     /locus_tag="VFD79_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93796.1"
                     /translation="MKVARIYIRTPDKSTVSLKKALLVKDAGIQGDKNARGGDRQLSL
                     LPLKVREDIEKGIVTGLCLPRFTENITYTGDSQLKEGERYLIGEAVIEISGTRKKCFS
                     ECENIIKNKPCILVKTVSYAKIISGGNVDENDEIQPFT"
     gene            complement(496..750)
                     /locus_tag="VFD79_06820"
     CDS             complement(496..750)
                     /locus_tag="VFD79_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93797.1"
                     /translation="MDNFTRRKLKKPGDLVEDIKSGDFSITKREFDLKRTKRTINELS
                     KDGKRIKERIDRLKEVHEQLLEKDNPDKRQFIDRKMPGKR"
     gene            complement(860..1681)
                     /gene="ccsB"
                     /locus_tag="VFD79_06825"
     CDS             complement(860..1681)
                     /gene="ccsB"
                     /locus_tag="VFD79_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004828624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HZH93798.1"
                     /translation="MDLVYFEELMFTIAFGGYTLSSILFFIFVAMKKERVGKAAIGLA
                     MAGFGIHTLAIVLRVIESGRLPFTNQFEFATSFAWGIVLCYLVLHFAYRFTGVGAFVM
                     PMAFLVMGYASMLPKEIKPLMPALQSSWLYIHVGTAVVSYGSFAVAAGLAAMYLIGER
                     QKASGDGKTVLPKSEVCDYLSYRVIAFGYLMLTLVIVTGAIWAKYAWSRYWAWDPKET
                     WSLITWLIYSVYIHLRFNRGWKGKPAAWFAVIGFISVIFTFIGVNNILPSIHSYR"
     gene            complement(1684..>2277)
                     /locus_tag="VFD79_06830"
     CDS             complement(1684..>2277)
                     /locus_tag="VFD79_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93799.1"
                     /translation="GEEWSDTMWVNNPLKVDRVDFFQTSYGWASSLSIIGPEGDEENG
                     LLRNGESYFYQPGHLTVYLYGYFPNMTLTSQGEPLTMTEQELNPYYAVILYEFGNHVA
                     SEILEPGQELQYKDYTLLFQDSELYTGITYRKDFGYIFVMIGSLLMTLGLIFSFYFYP
                     KHIQVEENSVRTITRQNTWGFEYQVRQLVQQSVEKEE"
BASE COUNT          583 a          574 c          448 g          672 t
ORIGIN      
        1 ctgtaaatgg ctgaatctca tcgttttcat ctacattacc acctgaaatt atttttgcat
       61 aactgacggt tttcactaga atacaaggct tatttttgat aatgttctcg cactcagaaa
      121 agcatttctt tcttgtccca ctgatctcta tgacagcctc cccgattagg taccgttctc
      181 cctccttcag ctgggaatct cccgtgtagg ttatgttttc agtgaacctg ggcaggcaaa
      241 ggcctgtgac gatacccttc tcaatatcct cccttacctt gaggggaagc agggaaagct
      301 gcctgtctcc acccctggcg tttttatctc cctggattcc cgcatccttt acaaggagtg
      361 ccttcttaag gctcactgtg cttttgtcag gagttctgat gtaaattctt gcaaccttca
      421 atttaccacc tcttccctga gtccatcctt ctggtgaagg taaaaaagaa aagaccaaca
      481 tagttgatct ctcaattatc tcttgccagg catttttcta tctataaact gtcttttatc
      541 agggttgtcc ttttccagaa gctgctcatg tacttccttg aggcggtcaa tcctttcctt
      601 gatcctcttg ccgtcctttg acagctcatt gatcgtcctc ttggtccttt ttaaatcgaa
      661 ttcccgcttt gtaatggaaa aatctccaga cttgatgtcc tccaccaggt ctccgggttt
      721 ttttagcttt ctccttgtaa agttatccat catatcaccc caagttatat ctaccctgtg
      781 gaaggatttc tgaatctgaa tatttccttc ctatatataa ggaaagacag ctttaagttt
      841 tccttaagct gtccattgac tatctgtagc tgtgaatgct tggcagtatg ttgttgaccc
      901 ctatgaatgt aaagattacg cttatgaaac caataaccgc gaaccatgca gccggttttc
      961 ccttccagcc cctgttgaag cgcaggtgga tgtagactga atatatgagc caggttataa
     1021 gtgaccaggt ttcctttgga tcccaggccc agtatcttga ccaggcatat tttgcccata
     1081 tggctcctgt aacgattaca agggtcagca taaggtagcc aaaagcaatc accctgtagc
     1141 ttaggtagtc gcaaacctca gactttggaa gaacggtttt tccatctcct gatgccttct
     1201 gcctttctcc aatgagatac atggcagcca gtcccgctgc aactgcaaag gacccgtagc
     1261 ttaccactgc agtcccaacg tgaatataaa gccaactgct ttgaagggct ggcataagtg
     1321 gttttatctc ctttggcagc attgaggcat agcccatgac aagaaatgcc attggcatta
     1381 caaaggctcc cactcctgtg aaccgatagg caaagtggag gaccagatag caaagtacaa
     1441 tcccccaggc aaagcttgtg gcgaactcaa attgattggt aaaggggagt cttccagatt
     1501 caatcaccct taaaacaatt gccagggtat gtatcccgaa tcctgccatt gcaagtccaa
     1561 ttgccgcctt cccgaccctt tccttcttca tggcgacaaa tatgaaaaac agaatcgacg
     1621 aaagtgtata tcctccaaag gcaatggtaa acatcagttc ctcaaaatat actagatcca
     1681 tccctattcc tccttctcaa ctgattgttg aacaagctgt ctaacctgat attcaaagcc
     1741 ccaggtattc tgtcttgtta ttgtccgtac gctgttttcc tccacctgta tgtgcttagg
     1801 atagaagtag aaggagaaaa tcaagcccaa cgtcattaaa agacttccaa tcatcacgaa
     1861 aatgtatcca aaatcctttc gatatgtaat tcctgtataa agctccgagt cttggaaaag
     1921 caaggtgtag tccttgtatt gaagctcctg gccaggctca agtatctcgg atgccacgtg
     1981 gtttccaaac tcatagagga tcacggcata atagggattg agctcctgct cagtcattgt
     2041 aaggggctcc ccctgggagg tcagggtcat gtttggaaaa tatccgtaca gatagaccgt
     2101 aaggtgaccc ggctggtaga aatatgattc cccattcctt aaaagaccat tttcctcatc
     2161 tccttcaggt ccaataattg aaaggctgct tgcccagcca tagctggtct ggaagaaatc
     2221 aaccctgtcc accttgaggg gattgtttac ccacattgta tcgctccatt cctctcc
//
LOCUS       DASRQF010000258         4753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1622462, whole genome shotgun sequence.
ACCESSION   DASRQF010000258 DASRQF010000000
VERSION     DASRQF010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1622462"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..872)
                     /locus_tag="VFD79_06835"
     CDS             complement(60..872)
                     /locus_tag="VFD79_06835"
                     /inference="COORDINATES: protein motif:HMM:NF015159.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HZH93800.1"
                     /translation="MRKILLVLLVFVLGTSLIGCGANEPEDNNEIKVGVAFYPMKDIL
                     LLIEDDLQEEGFDLVINEFSDYQVPNNLLQNGELDANMIQHDYFLQIFNESNDADLVT
                     VQPIYHATFALYSKDYASLDEIPEGASVTMADDATNFSRSLYLLGQAGLLTFNDGKTT
                     GLTVEDIASNPKNLQFEDKVPLTSLAQRYSETGLAVMYPTYARSLELVGDEQRLYVEQ
                     QDDVTQGYAISLVARDDNRDSEKIQALIKHITSDKVRDFLIEEYSWASKPAF"
     gene            complement(887..1561)
                     /locus_tag="VFD79_06840"
     CDS             complement(887..1561)
                     /locus_tag="VFD79_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZH93801.1"
                     /translation="MVERYIETFNRFRSPLVQSLWETFDMMLASMAAALVIGLVLGFI
                     MFMTGAGGIYKNRAVYFMTSGFVNITRSIPFILFIIVMIPVNRMILGTGFGVDASKIP
                     LSLIGVSLFARFTEQDLLDVNKNIFETSYAMGASTLQYFTHFLLREARSSLVLSFTST
                     TVSLLSYSTVMGIIGGGGLGYLAISEGYYNFNYELMWIIIILMVLIVHGIQTAGNNIA
                     KKLDKR"
     gene            complement(1554..2273)
                     /locus_tag="VFD79_06845"
     CDS             complement(1554..2273)
                     /locus_tag="VFD79_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZH93802.1"
                     /translation="MIRLEKVSKDFFIGGEKTRVIDGINLHVDKKEIFGLVGETGSGK
                     STILRMMNGFIEPDEGSIYLMGKLLNSDTRHELVKETSMIFQGFNLLGNLNVIENILL
                     PTRLRKGNQRENIGKARELLSFVGLSEFEEAYIRTLSGGQKQRVAIARTLITNPRIIF
                     CDEPTSALDEKMSYEILRLLKDINERFETTIVMVSHDIGVIKSVCSRVAVMEDGRISE
                     LISLTPKGIEPVSYKEAFLSG"
     gene            complement(2488..2871)
                     /locus_tag="VFD79_06850"
     CDS             complement(2488..2871)
                     /locus_tag="VFD79_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93803.1"
                     /translation="MRAYLGIKYHKDYRNGEVIEKILDILEGNGHETSCIVRDCELGR
                     ESCTNPADLMKETFKRIDSCDLVVIEFSEKGVGLGIEAGYAFARGIPVLTIAKEGSQV
                     SETLEGISSKVIKYRSLDELRDVKF"
     gene            complement(2868..3818)
                     /locus_tag="VFD79_06855"
     CDS             complement(2868..3818)
                     /locus_tag="VFD79_06855"
                     /inference="COORDINATES: protein motif:HMM:NF014345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HZH93804.1"
                     /translation="MCTGIKIDYDDGCVLGRTMDYESPMNYNVLYFPRNYNYGMDLMG
                     RPLYSKYRMLGVCFHNRDPLKDGVNEHGLVGITNDFKGFNLYNQKTNPERINVSSLDY
                     FNYALANYKSVQDLVDDLPNIHMATHNHLEEKVISPDFHFMFSDSTKRCVVIEPKGGE
                     LAWYDNPYHVMTNSPGFKSHVRRLNDHMDMENLEDFNSSKDLPGGYDPVSRFIKAFYM
                     TRMSKEASGYQEALSYFHSIMNAMTLPDGFIRNKKYSSTTYTRYICSYDTKRMEMTLK
                     ADTNPMVYRLSLDDIVDKDKRQEFFIQLEFNTKNLMESQI"
     gene            complement(4036..>4753)
                     /locus_tag="VFD79_06860"
     CDS             complement(4036..>4753)
                     /locus_tag="VFD79_06860"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZH93805.1"
                     /translation="LEEDTLVDETYIMDRIPGSRFHNGGRIKFGPDGKLYVTTGDAQV
                     PNLSQDVDSLAGKILRLNPDGSIPDDNPFEGSPVYAYGLRNPQGLAWHPISGALYASD
                     HGPSSQDEVNRIIPGGNYGWPIVTCGDGESQYEDPVACYWEFTMAPSGIGFLPWDNLE
                     ESPLYVGGLRGSMVIRIDLDPEGNLIRQDQLFDDYGRIRTVVYHEGSLYIATNNRDGR
                     GVVRENDDKILKITPALPVE"
BASE COUNT         1294 a         1137 c          817 g         1505 t
ORIGIN      
        1 atccatacaa accctcctat ttcgagtttt acattcacaa atctctttaa tgatatctct
       61 taaaatgcag gcttgcttgc ccagctgtat tcttcaatca ggaagtctct tactttatca
      121 ctagtaatat gcttgatcag ggcctggatt ttctcgctat ccctgttgtc atccctagct
      181 acaagggata ttgcatatcc ttgtgtgaca tcatcctgct gctcaacata gagtctctgt
      241 tcatccccaa ccagttcaag gctcctcgca taggttggat acatgacagc aagaccagtt
      301 tctgagtacc tctgagcaag gcttgtcaat ggaaccttat cctcaaactg gaggttcttt
      361 ggattgctgg ctatgtcttc tactgtcaag ccagttgttt taccatcatt aaatgtcaat
      421 agcccagcct ggcctagtag atagagggac ctgctaaagt ttgttgcatc atcagccatt
      481 gttacgcttg ctccttccgg aatttcatcc agggatgcat agtccttgga atagagtgca
      541 aatgttgcat ggtagattgg ctgaacagtt acaaggtcag catcattgga ttcgttgaat
      601 atctgcagga agtaatcatg ctgtatcata tttgcatcaa gctccccgtt ctggaggaga
      661 ttgttgggca cctggtaatc tgaaaactcg ttaataacca ggtcaaatcc ttcctcctgg
      721 aggtcatcct caattagcag tagaatgtcc ttcattggat agaatgcaac ccctaccttt
      781 atctcattgt tatcctcagg ttcatttgct ccacacccaa tcaaggatgt gccaagcaca
      841 aataccaaca aaaccaataa aatctttctc attgtcatca tctccattat cttttatcta
      901 attttttagc aatattgttt ccagctgttt gtatcccgtg gacaattaaa accatcagta
      961 tgatgattat ccacatcagt tcataattga aattatagta tccctcactt attgcaaggt
     1021 atcccagtcc acctccaccg atgattccca ttacagttga ataggacaag aggcttactg
     1081 tcgtggatgt aaagcttaaa accaggcttg accttgcctc tctaagcaaa aaatgtgtga
     1141 agtactgcaa ggtactggcc cccatggcat aggatgtttc aaaaatgttc ttgtttacat
     1201 ccaacaggtc ctgttcggtg aacctagcaa acagtgatac tcctatcaag ctaagtggaa
     1261 tctttgaggc atccactcca aatccggttc caagtatcat cctgttaact ggtatcatca
     1321 ctattatgaa cagtataaat ggtattgacc tggttatatt gacaaatcct gatgtcatga
     1381 aatatactgc cctgttcttg tatatccctc ctgctccagt cataaacatt ataaatccca
     1441 acaccaggcc tatgaccaag gctgcagcca ttgaagcaag catcatgtca aaggtctccc
     1501 aaagtgactg gacaagggga gatctgaacc tattaaatgt ttcaatgtat ctttcaacca
     1561 cttaaaaagg cctccttata tgaaactggc tctatcccct ttggtgttag ggagatcagt
     1621 tctgatatcc taccatcctc cataacagct acacggctgc agacagattt aatgacacct
     1681 atgtcatggg aaaccatcac aatggttgtt tcaaaccttt cattgatatc ctttagtagt
     1741 cttaggattt cataactcat cttctcatca agtgctgatg tgggctcatc acagaatatt
     1801 atcctaggat tggtaataag ggtccttgct attgcaaccc tctgcttctg tcccccagat
     1861 aaggttcgta tatatgcttc ctcaaactcc gataatccaa caaaagacag taactccctt
     1921 gccttcccaa tgttctccct ttggtttcct tttctcaatc tggtgggtaa aagtatattt
     1981 tcaatgacat taaggttccc caagaggtta aatccctgaa aaatcatgga ggtttccttc
     2041 accagttcgt gtctggtatc actgtttaac aacttaccca taaggtagat gcttccttcg
     2101 tcaggttcga taaaaccatt catcatcctg aggatggttg atttgccact tcctgtctct
     2161 ccaacaaggc caaatatctc cttcttgtct acatgaaggt tgattccatc aattactctg
     2221 gttttttcac cacctatgaa gaagtccttg gaaacttttt ctaatcttat caaaacatca
     2281 ccaccgatat atattccctt ccctaaccca actcttattt atccgataaa aaaagtatct
     2341 gaagatgcat tatggaaact ttttatgata ctcatacacg gaaatttcat gatttaaaaa
     2401 agtagagatt tcctattcgc aataaaaaga acacatccat tcccattaat acagtatagc
     2461 aatctttcaa tttaatgata ggataatcta gaatttaaca tctctcagtt catctagaga
     2521 cctatatttt ataaccttgc ttgatattcc ctccagagtc tctgatacct gacttccttc
     2581 tttagctatt gttaaaaccg gtattcccct ggcaaaggca tagcctgctt caatacccag
     2641 gccaactccc ttttcgctga attcaataac cacaagatca caggaatcga tcctcttaaa
     2701 tgtctctttc attaagtcag ctgggttggt gcaagattcc cttcccaact cacagtccct
     2761 aactatacag cttgtttcat gtccatttcc ctcaagtatg tctaaaatct tttcaatgac
     2821 ctcgccattt ctgtaatcct tatgatattt gattcccaaa taagccctca tatctgactc
     2881 tccataaggt ttttagtatt aaactccaat tggataaaaa actcctgcct cttgtcctta
     2941 tcaacaatat catcaaggct taatctgtag accattggat ttgtatctgc cttaagggtc
     3001 atttccattc ttttagtatc atatgaacag atataccttg tgtaggtggt ggagctgtac
     3061 ttcttattcc tgataaaacc atcaggcaag gtcatggcat tcattatgct atgaaagtat
     3121 gaaagcgcct cctgataacc tgacgcctct ttactcatcc tggtcatgta gaatgccttg
     3181 ataaacctgg acacagggtc atagccacct ggcaggtcct tggatgagtt gaaatcctca
     3241 aggttctcca tatccatatg gtcattaagt ctccttacat gggacttgaa tccaggagaa
     3301 ttggtcatga catggtaagg attgtcatac caggccagtt ccccaccctt tggctctata
     3361 accacgcatc ttttcgttga atcggaaaac ataaaatgaa aatccggtga aatcaccttt
     3421 tcttccaagt gattatgtgt cgccatatga atgttgggca agtcgtccac caggtcttgc
     3481 acagatttgt agtttgcaag ggcatagttg aagtagtcca ggcttgacac atttatcctt
     3541 tcagggttgg ttttctgatt gtataggtta aagcccttga aatcattggt tattccaacc
     3601 agtccatgct catttactcc atccttcaac ggatccctat tatggaagca aacccccagc
     3661 atcctgtatt tggaatatag tggtcgtccc ataaggtcca tgccatagtt gtagtttctt
     3721 ggaaagtaca atacattgta gttcatgggt gattcgtaat ccattgtcct tcccaataca
     3781 caaccgtcat cgtaatcaat ttttattcca gtacacatat aatccctctc tttgtaacta
     3841 gtcttaattt ctaccttgat cttttagttt gattatctct cttgtcggtc tgttaaatac
     3901 ctgcatatga ttctatcata tccctcaacg attataaaag ctggttgaaa aactccttta
     3961 gtaattaccc aaataagaat cccccagtca aaattctggg agattctcca tattccttat
     4021 gattgttccg aaatattatt ctacaggcaa ggcaggtgta atcttgagga ttttgtcatc
     4081 attctccctg acaactcctc ttccgtccct attgtttgtc gcaatgtata atgagccttc
     4141 atgataaacc actgttctta ttcttccgta atcatcgaaa agctggtctt gacggataag
     4201 gttaccctca ggatcaaggt ctattctaat gaccatgctt ccccttaagc ctccaacata
     4261 caatggggat tcctccaaat tgtcccaggg caggaagcct attccggaag gtgccattgt
     4321 aaattcccag tagcaggcaa cggggtcctc atattgagac tctccatccc cacaggtaac
     4381 gattggccat ccgtaattcc ctccagggat tatcctgttt acttcatcct ggctgcttgg
     4441 tccatggtca ctagcatata atgctccact tattggatgc catgcaagtc cctggggatt
     4501 tctaagccca tatgcatata caggacttcc ctcaaatgga ttatcatcag ggatgcttcc
     4561 atctggatta agtctaagaa tctttcctgc cagagagtcc acatcctgtg acaggtttgg
     4621 gacctgggca tcacctgtgg taacataaag ctttccatca ggtccaaact taatccttcc
     4681 accgttatgg aaccttgacc ctggaatcct gtccatgata tatgtctcat caaccagggt
     4741 atcctcctca agt
//
LOCUS       DASRQF010000259        11298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1159731, whole genome shotgun sequence.
ACCESSION   DASRQF010000259 DASRQF010000000
VERSION     DASRQF010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 11298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11298
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159731"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VFD79_06865"
     CDS             <1..275
                     /locus_tag="VFD79_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HZH93806.1"
                     /translation="LTTKGEALHVCDEFQESALREADTIAILKTNKDKGEILEALERE
                     RFQITYIRKATLEDERIIRDVDEIIEEDAYMSMIIGKRQKGELKCT"
     gene            275..1285
                     /locus_tag="VFD79_06870"
     CDS             275..1285
                     /locus_tag="VFD79_06870"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZH93807.1"
                     /translation="MTKIRILLLVLAVSMAASGCSINAMEEADTNGSNETAVIGKEDE
                     TDRYMIEDMGDSVSFVDGRGEKLQVEKNPERVIVLMNSFVDMWVENGGEMVGMVEDPN
                     NQVAGTENVETVGRFGSISLEKVISLEPDLVILSSNSKSSTELIQPLEENGIPVMPLT
                     YEFKEDYFKISKLFALINDRVDLYEENAEKVETDIREIIDGIPEGNNPSVLILFATSK
                     SVTARGSLSTLGEMFKDLKVRNVAEGEDLMETVQFSMEQVIAEDPDFILVQTMGSDME
                     AVNERLVSEVESNPAWKELSAVKNKNYVLLPKDLYTYKANRRYGEAYRGLAEMLYPEI
                     FN"
     gene            1295..2299
                     /locus_tag="VFD79_06875"
     CDS             1295..2299
                     /locus_tag="VFD79_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HZH93808.1"
                     /translation="MEFNGNIKKSNILLAMAVAFVATIFVSIGKGAVQVGVSEIVEAL
                     IFEEITPNKQIIWNVRFPRTVAAILVGVALSLSGAILQGVMRNPLAGPNIIGVSAGGG
                     FFGLLIMIIFPNYFFLVPVGAFIGALLATTIIYFLAWKDGVMPSRLILAGVAISSIFG
                     AGNNALMIFYPERVSGVLGFMVGGLSAITWKDVNMVYPFILMGVAASLVLPGRLNILL
                     LGDEVATGLGMRVERTRLFAIVISSLLAGAAVSVVGMLGFVGLMVPHITRLFLGSDYR
                     YLVPGSMLMGGTILLLCDTIARVLFAPMEIPVGIIMSALGAPFFLYLLRKGKGHHHGA
                     "
     gene            2289..3080
                     /locus_tag="VFD79_06880"
     CDS             2289..3080
                     /locus_tag="VFD79_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZH93809.1"
                     /translation="MELRVSGLEVLYGNKIILKDIHMEAKEGEIVSVIGPNGSGKSTL
                     VKTVSRLLRPKAGEISYSGRSINSIPTRELARMVAVLPQEKKLNVDLTVEQLVSYGRH
                     PHLGFGNRFGKRDREIVVEAIRLTGMEEHREKSMHNLSGGERQRAWISMALAQQPRVL
                     ILDEPTTYLDISYQMEVLELVEELNKSLGITVLMVLHDLNHACRYSDKVYVLKDGRVH
                     GYGSPSEVVDRKMLKEVFGIEARIYEDEVNNCRFFIPGRIAKEGA"
     gene            3082..4143
                     /locus_tag="VFD79_06885"
     CDS             3082..4143
                     /locus_tag="VFD79_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase"
                     /protein_id="HZH93810.1"
                     /translation="MEHGGDIYTYGRGFEGEIIDFSSNINPLGTPTGLREHLAGGHED
                     LLKYPDIQYRELRREVAEYLGCGIENTIVGNGAVEIIDNFIMKANRVVLMEPCFSEYM
                     LRAKVHGKEIVLLASSKDYTLDFDLFPGKLEKGDLVILGNPNNPTGMRIERGDLIKLH
                     QEALYSGAYLLLDEAFFEFAPDDYDSIELFREWGWESVGIIRAATKFFALPGIRLGYC
                     CAGAVKAEGIREIQLPWSVNSMANKAGLHIFKDGDYIVRTKKYVKEQRERLFEGLRQI
                     KGITPYPSQSNYILIRLDGVREDYIFEKLLEKGIMVRKCRSFRVLSDNHIRIAVKDEK
                     NNEKFINELRGIMAFEDEE"
     gene            4164..4892
                     /locus_tag="VFD79_06890"
     CDS             4164..4892
                     /locus_tag="VFD79_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014092591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HZH93811.1"
                     /translation="MLRKTIFAAFVGLMLLVTPGVSHGMHIMEGFLPLKHALFWGAIS
                     LPFFIGGVFSLKRSLKKDDNAKLYLGLAGGFVFVLSALKIPSITGSCSHPTGVGLSTI
                     IFGPLATTVIGTIVLLFQAILLAHGGLTTLGANMFSMAIAGPFVSFGIFRLLGKSGVK
                     QSYVVFLAASLGNLSTYMVTALQLALAFPEGVGGFGGALAKFMSIFAVTQIPLAVIEG
                     ILTVMVYSMIAEYSSLKGSSLMAH"
     gene            4948..5238
                     /locus_tag="VFD79_06895"
     CDS             4948..5238
                     /locus_tag="VFD79_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HZH93812.1"
                     /translation="MKSFWSRNLLLGILVVLIALVPLLKDDGAEFGGTDGEAEEAVME
                     MNPGYEPWAEPLWEPPSGEIESLLFSLQVALGAGAIGYVIGVMKERKKFAPY"
     gene            5225..5908
                     /gene="cbiQ"
                     /locus_tag="VFD79_06900"
     CDS             5225..5908
                     /gene="cbiQ"
                     /locus_tag="VFD79_06900"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HZH93813.1"
                     /translation="MLPIDRYAYTNGIADLDPIIKLAVCGATIIVSMVFESYVLNWLL
                     FTVMVAVTTIVARVPLIFYLKVLMVPMVFLVMGFIGIVISVSTMEVFTWSIGIGSYNF
                     GITMESYELGLLTVSRSAAAISGTLFLALTTSADSILRKVWKYPIPKVLLEIAILTYR
                     SIFIFLEEASVMHQAQAMRHGHDGIEKSFLSWSLLVKEIFTRVLKRYEAMIVSLETRN
                     YNGQFKMGD"
     gene            5909..6709
                     /locus_tag="VFD79_06905"
     CDS             5909..6709
                     /locus_tag="VFD79_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HZH93814.1"
                     /translation="MIKVKELSYIYEDGTQALKNIDMDFSKGRTVGIIGANGSGKSTL
                     LLAIMGVLRSSVGSITVDGQMLGKNRNSLRDHRKRVNMVFQDPDSQIFYSRVYDDVAF
                     GPRNLDMNEAEVSRRVEKSLGEVQMRDLKDKPVHFLSFGQKKRVSIAGVLSMEPQMII
                     MDEPTSGLDPKMKEELEKIIRNQSKERRIVLSSHDMDFVYEICDYVYVIRQGEVIGEG
                     VPREVFLDRELLARASLNMPWLVRIHLELGLPLWVSSREISGKGMEVL"
     gene            6709..7479
                     /locus_tag="VFD79_06910"
     CDS             6709..7479
                     /locus_tag="VFD79_06910"
                     /inference="COORDINATES: protein motif:HMM:NF017944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016852 - sirohydrochlorin
                     cobaltochelatase activity [Evidence IEA];
                     GO_process: GO:0019251 - anaerobic cobalamin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin cobaltochelatase"
                     /protein_id="HZH93815.1"
                     /translation="MKKGIIVASFGTTHQDTREKTIDVIEHKIRKEFSDYPVVGAFTS
                     GVVIRRLREDYGIQVPDMKEAMEQLASEGVTDIYVQPLHIIAGIEFEKVLRAARDFMK
                     NTGKVIVRVGTPLMWEDRDFRNIVKILIPKDDASVVFMGHGSRHKADTSYERLAEHFG
                     RAGIRNVHIGTLEGKTGLEDVMGKLKEGGNSQVLLKPFMLVAGDHAKNDMASEDEDSW
                     SSILEREGFNVSIDMTPLGEIEEIQRMFVTKMKEVIRS"
     gene            7476..8798
                     /locus_tag="VFD79_06915"
     CDS             7476..8798
                     /locus_tag="VFD79_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HZH93816.1"
                     /translation="MKGFMIAAPSSNTGKTTITTGIIRALEKQGYFVSPYKTGPDYID
                     GKFLSMAAGTRAGNLDLHLQGEEGVREALSMGKGDIAVIEGVMGYFDGSSNTWEKSSY
                     DISKRLDIPVVLVYTPKGEMFSAVPKIKGMAEFEDSRIQGVILNKTSKRMYEMLKEAI
                     ENHTSLSVIGHIPYDQDLEIDSESLGLLEPGRITGLEKKIERMAELVQENLDMEKLLS
                     IARDLDISRAAKLDKGDMRVAVAMDDAFSFHYKENLELLRSMANVCFFSPVYDRALPE
                     ADLVILGGGYPEKYKERLSENRSMILSIREYGEGGGHILAEGGGMLYLMDTLEGVEMC
                     GLLKGDARMTDRLQRFGYVNISIGEDCVLGKAGTEIPANEFHKSVVENTDEPIFRINK
                     PLGNHSWACGYKRKNVLGYFQHLNLAGKSSLLKGILDEVISGGGKDVY"
     gene            8788..9408
                     /locus_tag="VFD79_06920"
     CDS             8788..9408
                     /locus_tag="VFD79_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009430299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HZH93817.1"
                     /translation="MYIKKPMEIENRSMEIIRKAIVDPGFSPKELQIAMRMVHTTGDF
                     DYSGIIDFRNDFIEKALSSIREGGIIFTDTKMALSGINKKALDKAGCEVRTFIDNEKV
                     LDMSREKGTTRSACAVDLALEKGIRMFVVGNAPTALFRILEHVEEGSLDPSFVVGVPV
                     GFVGAAESKEKLREFGIPSISTVGTKGGSNVAASIINALLYMMVER"
     gene            9405..10484
                     /gene="cbiD"
                     /locus_tag="VFD79_06925"
     CDS             9405..10484
                     /gene="cbiD"
                     /locus_tag="VFD79_06925"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase
                     CbiD"
                     /protein_id="HZH93818.1"
                     /translation="MIYNGLGYVIKDGKKLRCGYTTGSCAAGAAKAAAMMLRGEEPDS
                     VEIDTPAGVLLNLVINSRRMEKHQAVCSIIKDAGDDPDVTDGIEIFARVRKRKDGRVV
                     ITGGEGIGIITRKGFFGEAGEFAINKVPREMIRSELEKVGDSGYDVEVFAPAGVEIGK
                     RTFNENIGIEGGISIIGSTGIVQPMSEKALLDTIFLEMDQIKLDHGTDGLLLVPGNHG
                     EKIARDEGISLPSVKISNFVGESLQYAYGIGFKSLVVLGHVGKLSKLSIGAFNTHSRV
                     CDTRLESFVYHLALMEAPLEFLKRVEACLTAEEAIHLCIDSGFGEIVKRMEESAEERV
                     RRYLKAEKVDIKVIVYSMERGVNLC"
     gene            10478..11074
                     /gene="cbiE"
                     /locus_tag="VFD79_06930"
     CDS             10478..11074
                     /gene="cbiE"
                     /locus_tag="VFD79_06930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HZH93819.1"
                     /translation="MLSVVGAGPGNPKYLTEEAIGIIGRADRVIAFGRIAESIRSIRK
                     DVVRISSVDQVEMELEHEGNIVLLASGDPTFYGIVGYLRKNGIKADSIVPGISSMQYM
                     FSILGLEWQDAYLSSAHGRKLELDSILERNLSVVLLDKENTPDMISTKLYEAGARGRI
                     HAGSNLSYDDEMIISCMIGDRIQMESNLTMVVIENENN"
     gene            11061..>11298
                     /locus_tag="VFD79_06935"
     CDS             11061..>11298
                     /locus_tag="VFD79_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6Y C5,15-methyltransferase
                     (decarboxylating) subunit CbiT"
                     /protein_id="HZH93820.1"
                     /translation="MKIIRDSEFIRGDIPMTKFVTRTVVMGLLQPDNNELLLDVGAGT
                     GSVSVQAAIHGARVVAIEKEKSGVDLINRNKERFN"
BASE COUNT         3360 a         1944 c         3061 g         2933 t
ORIGIN      
        1 ctttaaccac aaaaggggaa gccttgcatg tttgcgatga gttccaggag tcagctctta
       61 gggaagctga cacgatagct atcctaaaga caaacaagga taagggagaa atcctggaag
      121 cacttgaaag ggaaaggttt cagattacat atatcagaaa ggcaaccctg gaagatgaga
      181 ggataattag agatgtcgat gagattatcg aagaggatgc ctacatgtca atgattatag
      241 gcaaaagaca gaaaggggaa ttgaaatgta cataatgaca aaaataagaa tattgctcct
      301 ggttcttgct gtttcaatgg cagcttcagg atgttccata aatgcaatgg aagaggcaga
      361 cacaaatgga tccaatgaga ctgccgttat tgggaaggaa gatgaaaccg atagatacat
      421 gattgaagac atgggtgata gtgtaagttt tgtggatgga agaggggaga aacttcaagt
      481 ggagaagaat cctgaaaggg tcattgtcct tatgaactca tttgttgaca tgtgggttga
      541 gaatggcgga gagatggtgg gcatggtaga ggatccaaac aaccaggttg ctggcaccga
      601 gaatgttgaa accgtaggaa gatttggatc aatcagcctt gaaaaggtaa tttcacttga
      661 acctgacctt gtgatcctaa gctcaaattc aaaatcatca acagagctga tccaaccgct
      721 ggaagagaat ggtattccag taatgcccct tacatatgaa ttcaaggagg actacttcaa
      781 gatttcaaag ctgtttgcac taataaatga cagggtggac ctatatgagg aaaatgcaga
      841 gaaagttgag actgatataa gggaaatcat tgatggcatc ccagaaggga acaatccctc
      901 agtgttgatt ctttttgcaa catccaagtc ggtcactgca agggggtcac tttcaactct
      961 tggagagatg ttcaaggact tgaaggtccg gaatgtagcc gaaggagagg atttgatgga
     1021 gactgttcaa ttcagcatgg agcaggtcat tgctgaagat cctgacttca tacttgtcca
     1081 gaccatgggt tctgatatgg aggctgtgaa tgagagattg gtttcagaag tggaatcaaa
     1141 ccctgcatgg aaggaacttt ctgcggtcaa gaacaagaac tatgtccttc tcccaaagga
     1201 cctttatacc tataaggcca acagaagata tggagaggca tacagaggtc tagcagaaat
     1261 gctctaccct gaaatcttca actaagggat agagatggaa tttaatggca atatcaagaa
     1321 atcaaatatc ctattggcaa tggccgtggc gtttgtcgcg acaatctttg taagtatcgg
     1381 caagggggct gttcaggttg gagtatccga gattgttgaa gcactgatat ttgaagagat
     1441 cactccaaat aaacagataa tatggaatgt tagatttcca agaacagtcg cagctattct
     1501 agtgggagtt gcactttcac tgtcaggggc aatactccag ggagtcatga ggaatccctt
     1561 ggctggaccc aacataattg gagtttctgc aggaggggga tttttcggtt tgctgataat
     1621 gataatattc cccaattatt tttttcttgt accagttgga gccttcatag gtgccctttt
     1681 agccacgaca atcatatatt ttctcgcttg gaaggatggg gtgatgcctt cgaggctaat
     1741 attggcaggg gtggccattt cctccatttt tggggcggga aacaatgcct tgatgatctt
     1801 ctatcctgaa agggtatccg gagttttggg atttatggtg ggaggattgt cggcaataac
     1861 ctggaaggat gtgaacatgg tctatccctt tatacttatg ggggttgccg catccctggt
     1921 gttgcctgga aggctcaaca tcctacttct tggtgatgag gtggcaacag gtcttggaat
     1981 gcgggttgag agaacacggt tgtttgcaat tgtaatctct tcacttcttg ctggagccgc
     2041 agtatcagtg gttggaatgc ttgggtttgt gggtttgatg gttccccata taacaagact
     2101 ttttctgggg tcggactaca gatatcttgt tccaggatcc atgcttatgg gaggaaccat
     2161 actcctcctt tgcgatacca ttgccagggt gctgtttgca cccatggaaa taccggtggg
     2221 aataataatg tcagcactgg gagcaccgtt ttttctgtat cttcttagaa agggaaaggg
     2281 gcatcatcat ggagcttagg gtaagtggac tggaggtcct ttacgggaac aagattatac
     2341 ttaaggatat tcatatggag gccaaggagg gtgaaattgt ttcggtgatt ggtcccaatg
     2401 gctctgggaa gtcgacgctt gtaaagactg tttccaggct cttaagacca aaagctggag
     2461 aaatctctta ttcaggcagg agcatcaatt caattcccac aagggagctg gcaaggatgg
     2521 ttgcagtgtt gccacaggaa aaaaaactaa atgtggacct aacggtggag cagcttgttt
     2581 cctacggaag gcatccccat cttggcttcg ggaataggtt cggaaaaagg gatagggaaa
     2641 tagttgtgga agctataagg ctgactggaa tggaggagca cagggaaaag agcatgcaca
     2701 acctttcagg aggggaacga cagagggcat ggatatccat ggctcttgcc caacaaccaa
     2761 gagttcttat tcttgatgag cctacaacat atcttgatat atcatatcag atggaggttc
     2821 tggagcttgt ggaagaactt aacaaaagtc tggggattac agttcttatg gttctccatg
     2881 atctcaatca tgcatgtagg tattcagaca aggtatatgt acttaaggac ggaagagtcc
     2941 atgggtatgg tagccccagt gaagtggtcg atcgaaaaat gcttaaggaa gtttttggaa
     3001 tagaagcaag gatatatgag gatgaagtca acaattgcag atttttcata ccaggcagga
     3061 tagcaaagga gggagcgtaa aatggagcac ggaggagata tttatacata cggcagaggg
     3121 tttgagggag aaataattga cttcagcagc aatataaatc cactgggtac accgactggg
     3181 ctaagagagc atctggccgg aggtcatgaa gaccttctaa agtatccaga catacagtac
     3241 agggaactta gaagagaggt tgcagagtat cttgggtgtg gcattgagaa caccattgta
     3301 ggtaatggag cagttgaaat aattgacaac tttataatga aggcaaatag agttgtcctt
     3361 atggagccct gtttttctga gtacatgtta agggccaagg tacacggcaa ggagattgtc
     3421 ctgctagcat ccagcaagga ttataccctt gattttgatt tattcccagg aaaactggag
     3481 aagggagacc ttgtaatcct tgggaatccc aacaatccta caggaatgag gattgaaagg
     3541 ggggatctta ttaagctgca ccaggaagcc ctttactcag gggcatatct acttttggat
     3601 gaggctttct ttgaatttgc tccagatgat tatgactcca ttgaactctt tagggaatgg
     3661 ggctgggaat ccgtaggaat aattagagca gcgacaaaat tcttcgcact gcctgggata
     3721 agacttgggt attgctgcgc tggagcagta aaggctgaag gaataagaga aatccagctt
     3781 ccctggagcg tgaactccat ggcaaacaag gcaggcctcc atattttcaa ggatggggac
     3841 tatattgtaa gaaccaaaaa gtatgtaaaa gaacaaagag aaaggctatt tgagggactt
     3901 agacaaatca agggcataac tccttatcct tcacaatcca attatattct tataaggctt
     3961 gatggagtaa gagaggatta tatttttgaa aagcttctag aaaaagggat aatggtcaga
     4021 aaatgcaggt ctttcagggt tctttcagac aatcatatac ggatcgccgt gaaggatgaa
     4081 aagaacaatg aaaaatttat aaatgagctt agagggataa tggcttttga ggacgaggaa
     4141 taaaacatga agggagtatg aaaatgttaa gaaaaactat ctttgcagca tttgttggtt
     4201 tgatgcttct tgtgactcct ggggtaagtc atgggatgca cataatggag ggctttctac
     4261 cattgaagca tgcattgttt tggggtgcca tatctttgcc cttctttatt ggtggtgtct
     4321 tcagcctgaa aagaagttta aaaaaggacg ataacgcaaa gctttacttg ggcttggcag
     4381 gaggatttgt atttgtcctg tctgccctga aaatcccctc cataacagga tcctgttcac
     4441 acccaacggg agttggacta agtacaatca tattcggccc cttggcaaca actgtcattg
     4501 ggaccattgt acttctcttt caggcaattc tgcttgctca tggcggatta accaccttgg
     4561 gagcaaacat gttttccatg gctatagctg gtccttttgt atcctttgga atattcaggc
     4621 tgttgggcaa atctggagtg aaacaatcat acgttgtttt tctagcagcc tcacttggca
     4681 acctatctac atatatggtg actgcattgc agcttgcttt ggcatttcca gagggagtag
     4741 gtggatttgg gggagcactt gcaaagttca tgtccatatt tgctgtgaca cagattccat
     4801 tggcggtaat cgagggaatt ctaacagtca tggtatacag catgattgct gaatactctt
     4861 ccttgaaagg ttcttccttg atggcgcatt gaaagttgga actgaaaact ataattaatg
     4921 ttcaaactaa ggagaggagc tgagaaagtg aagagttttt ggagtaggaa cctgttgttg
     4981 ggcatattgg ttgttttaat agcattggtg ccccttttga aagacgacgg tgcagaattt
     5041 ggagggacgg atggagaggc tgaagaggca gtgatggaga tgaatcctgg atatgagcct
     5101 tgggcagagc ccttgtggga gcctccaagc ggagaaatag agagtctttt gttctctctt
     5161 caagttgcat tgggagcagg agccatcggt tatgtaatag gagttatgaa ggagaggaaa
     5221 aagtttgctc cctattgata gatatgcata tacaaacgga attgcagacc tggatccaat
     5281 cataaagctg gcagtctgcg gagcgacaat tattgtatcc atggtgtttg agagctatgt
     5341 cctcaattgg ttgcttttca cggtgatggt ggctgtgaca acaatagttg ctagggtacc
     5401 cttgattttc tatctcaagg tgctcatggt accaatggta tttctggtta tgggtttcat
     5461 tggaatcgtc attagtgttt caactatgga agtgtttacc tggtccatag gcattggctc
     5521 ttataatttt ggtattacaa tggagtccta tgaacttgga cttttgactg tatccaggtc
     5581 agctgcagcc atctcgggta ctttatttct ggcattgacc acctctgcag acagcattct
     5641 tcgaaaggta tggaaatacc caattccaaa ggtgcttttg gaaattgcca tactcacata
     5701 caggagcata ttcatattcc tggaagaagc ttctgttatg catcaggcac aggcaatgag
     5761 acatggacat gacggtattg agaaatcatt tttgtcatgg tctttgttgg tcaaggaaat
     5821 attcacaagg gttctaaaaa ggtacgaagc catgattgtt tcattggaaa ccaggaacta
     5881 caacggacaa ttcaagatgg gtgattagat gataaaagtg aaggaattaa gctacatata
     5941 tgaagatgga acacaagcat tgaagaacat agacatggat tttagcaagg gtagaaccgt
     6001 gggtattata ggtgccaacg gttcagggaa atcaactctt cttcttgcta ttatgggggt
     6061 gctgaggtcg tctgtaggta gtattactgt tgatggacag atgcttggca aaaataggaa
     6121 ctccctgaga gatcacagga aaagagtgaa catggtgttt caggaccctg acagtcagat
     6181 attctattca agggtatatg acgatgtggc ttttggtccc cgcaacttgg atatgaatga
     6241 ggctgaagtt tccagaaggg ttgaaaagtc ccttggagaa gttcagatga gagaccttaa
     6301 ggataagcca gttcattttc taagcttcgg tcagaaaaag agggtttcca tagcaggtgt
     6361 tctctcgatg gaaccacaga tgataataat ggatgagcca acatctggac tggatccaaa
     6421 gatgaaggaa gaattggaga agattatacg aaaccaatcc aaggagcgac ggatagtatt
     6481 atcaagccat gatatggatt tcgtttatga gatctgcgat tatgtgtatg tcataagaca
     6541 gggtgaagta attggagagg gagtgcctcg ggaagtgttt cttgataggg agcttctggc
     6601 cagggcaagt ttgaatatgc cttggctggt aaggatacac cttgagctgg gtcttccact
     6661 ttgggtaagt agcagagaga tttcaggaaa aggtatggag gtgctttgat gaaaaaggga
     6721 attatagtgg caagttttgg aacaacccat caggatacca gggagaagac aattgatgtg
     6781 atagaacata aaataaggaa ggaattttcc gactatcctg tggtaggggc cttcacctca
     6841 ggagttgtaa tcagaaggct aagggaggat tatgggatcc aagtcccaga tatgaaggaa
     6901 gccatggagc aactggcatc tgaaggagtt acagatattt atgtgcagcc tttgcatata
     6961 atagcaggga ttgaatttga gaaggttcta agggctgcca gggactttat gaaaaatacc
     7021 ggcaaggtca ttgtaagagt tggcactccc cttatgtggg aggacaggga tttcaggaac
     7081 atagtaaaaa tactcatccc taaggatgat gcttcagtgg ttttcatggg tcacggatca
     7141 agacacaagg ctgacacttc ctatgaaaga ctagcagaac actttggaag ggcgggtatc
     7201 agaaacgtac atataggtac tcttgaaggg aaaactggac tggaagatgt gatgggaaag
     7261 cttaaagaag gaggcaatag tcaggtctta ctaaagccat ttatgctggt ggctggagac
     7321 catgcaaaga acgatatggc atctgaagat gaggattcct ggagtagcat cctggaaagg
     7381 gaagggttca atgtgagcat tgatatgacc cctcttgggg agattgagga aatacagagg
     7441 atgtttgtca caaagatgaa ggaagtgatt aggtcatgaa ggggtttatg atagcagcac
     7501 catcaagcaa caccgggaag accaccatta ccaccggaat aattagggcc ttggagaaac
     7561 agggatattt tgtaagtcca tacaagacag gaccggatta tatagacggc aagtttcttt
     7621 ccatggctgc aggtaccaga gctggtaacc tggatttgca tcttcaggga gaggaagggg
     7681 tcagggaagc actttcaatg ggaaagggag atatagctgt aattgaagga gtgatgggat
     7741 attttgatgg ttcctcaaac acatgggaga aatcatccta tgacatttcc aaaagacttg
     7801 atatacctgt ggttcttgtg tacacgccca agggggagat gttctcagct gttcccaaga
     7861 tcaaggggat ggctgagttt gaggattcaa gaatccaagg ggtaatcctc aacaagacca
     7921 gcaaaaggat gtatgaaatg cttaaggaag ccattgaaaa ccatacaagt ctttcagtca
     7981 taggccatat accatatgac caggatcttg aaattgatag cgagtccttg ggactacttg
     8041 aacctggtag gataaccggg ctggaaaaga aaatcgagag gatggctgaa ctggtccagg
     8101 aaaacctgga tatggaaaag ctcctttcaa ttgcaaggga tcttgacatt tcaagagcag
     8161 ccaagcttga caagggagat atgagggttg cagtcgccat ggatgatgcc ttctccttcc
     8221 attacaagga gaatttggag ctattgaggt caatggcgaa cgtgtgcttc ttctccccag
     8281 tctatgacag ggcccttcct gaagccgacc tggtcatact gggaggtggc tatcctgaga
     8341 agtacaagga aaggctatct gaaaacagat ccatgatcct ttcaatcaga gaatatggag
     8401 agggtggtgg tcacatactg gctgagggag gcggtatgct gtaccttatg gataccttgg
     8461 aaggagttga aatgtgcggc cttctaaaag gagatgcgag aatgactgac cgacttcaaa
     8521 ggttcggata cgtgaatata tcaatagggg aagactgtgt tcttggtaag gcgggaacag
     8581 aaatacctgc caatgaattt cacaaatcag tggttgagaa tacggatgaa cctattttca
     8641 gaatcaataa accgctggga aatcacagct gggcctgcgg atacaaaagg aaaaatgttt
     8701 taggctattt ccagcacctc aatcttgccg gaaagagttc gcttctgaag ggaatactgg
     8761 atgaagtgat cagtggaggt ggaaaggatg tatattaaga agcccatgga gatagaaaat
     8821 aggagcatgg aaatcataag aaaagccata gtggatccag gattttcacc taaagagctc
     8881 cagatagcca tgaggatggt ccataccacg ggtgattttg attacagtgg cataattgac
     8941 tttaggaatg attttataga aaaagctctc tcctcaataa gggagggtgg aattatcttt
     9001 acggatacta aaatggcact ttccggaata aataagaagg ctttggacaa ggcaggctgt
     9061 gaagtcagga ccttcatcga taatgaaaag gtattggata tgtcccgaga aaaaggcacc
     9121 acaagatcag catgtgcagt tgacctggcc cttgagaagg gaattcgcat gtttgtggtt
     9181 gggaatgcac ccactgccct tttccggata cttgagcatg tggaggaagg gagccttgac
     9241 ccaagttttg tagtgggagt acctgtaggc tttgtgggag cggctgaatc caaggagaag
     9301 ttaagggagt tcggcatccc aagcatttca acagttggaa caaagggagg tagcaatgtg
     9361 gcggcttcca taataaatgc cctcctatat atgatggtgg aaagatgatc tacaatggtc
     9421 tgggatatgt aatcaaggac ggaaaaaagc taaggtgcgg atatacaacc ggaagctgtg
     9481 ctgcaggtgc ggcaaaggct gccgcaatga tgctgagagg tgaagaacct gactctgttg
     9541 aaattgatac acctgcaggt gtgcttttaa atcttgtaat aaacagcaga agaatggaga
     9601 agcaccaggc ggtgtgttca attatcaagg atgccggaga tgacccggat gtcactgacg
     9661 gaattgaaat ctttgccagg gttagaaaga gaaaggatgg aagggttgtc ataacaggtg
     9721 gagaaggcat agggataatt accagaaaag gattttttgg tgaagcgggg gaattcgcaa
     9781 tcaacaaggt tcccagggag atgataagaa gtgaactgga aaaggtgggg gattcgggct
     9841 atgatgttga agtttttgcc cctgcaggtg ttgaaattgg aaagcgtacc tttaacgaga
     9901 acatcggtat agagggtgga atttcgatta tcggatccac tggaatagtt cagccaatga
     9961 gtgaaaaagc ccttttggat accatatttc tggagatgga tcagattaag cttgatcatg
    10021 gtacagatgg tctgcttctt gtccctggga accatgggga gaaaatcgcc agggatgagg
    10081 ggataagcct gccctctgta aagatatcaa actttgtggg tgagtcgctg cagtatgcat
    10141 atggaatagg tttcaagtcc ctggtggttc tcggacatgt tgggaagctt tcgaagctta
    10201 gcatcggagc ctttaataca catagcaggg tatgcgacac caggcttgaa tcatttgtct
    10261 accaccttgc cttgatggaa gctcccctgg aatttcttaa gcgggtggag gcctgtttaa
    10321 ccgctgagga ggcaatccat ttgtgcattg acagtggctt tggtgaaatt gtcaagagaa
    10381 tggaagagtc cgcagaggag agggtaagga gatatctgaa ggctgagaaa gtggacatca
    10441 aggtaattgt ctactctatg gaaaggggtg tgaacctatg ctgagtgtag ttggtgcagg
    10501 gcctggaaat ccaaaatacc ttacggaaga ggccattggc attattggga gagcggatag
    10561 ggtaattgcc tttggcagga ttgccgagag cataaggtcg atcagaaagg acgttgttag
    10621 gatcagttca gtagaccagg tggaaatgga gcttgagcat gaagggaata ttgtgctgct
    10681 tgcttctgga gatcccacct tctatggaat cgttggatat ttaaggaaaa atggaataaa
    10741 ggcagatagt atagtccctg gaatatcctc catgcaatac atgttctcaa tccttggatt
    10801 ggaatggcag gatgcctacc tttcctctgc ccatggcagg aagcttgaac tggactcaat
    10861 tcttgaaagg aacctctcgg tggtcctgct ggacaaggag aacacaccgg atatgatttc
    10921 cacaaagctc tatgaagcag gggccagagg caggatacat gccggaagca acctatccta
    10981 tgatgacgaa atgataataa gctgcatgat tggagatagg atccaaatgg agtcaaattt
    11041 aactatggtg gtgatagaaa atgaaaataa ttagggacag tgagttcata aggggagata
    11101 tacccatgac aaaatttgtc acaaggaccg tggtcatggg tctgcttcag cctgataaca
    11161 atgaactgct tcttgatgtt ggagcgggaa caggatccgt ttctgtgcag gctgcaatcc
    11221 atggcgccag ggtcgtggca attgagaagg aaaaaagtgg agttgacctt ataaacagaa
    11281 acaaggaaag gttcaatg
//
LOCUS       DASRQF010000260         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1200901, whole genome shotgun sequence.
ACCESSION   DASRQF010000260 DASRQF010000000
VERSION     DASRQF010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200901"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..746
                     /locus_tag="VFD79_06940"
     CDS             3..746
                     /locus_tag="VFD79_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HZH93821.1"
                     /translation="MLALKDSYRDGTIILNEEVAGGVFKLVLDGDFTGAPGQFYMLRG
                     WNGLDPFLPRPISIGDVQDGRLTLLYEVRGRGTHIISKLSPGDSLSVLGPLGNGFQIY
                     REGRIALVSGGIGIAPLLYLAKNTEAEVDLYSGFRSEPYFLDEFKPFVNEIKVSTEDG
                     SLGHQGFVTELLRPEAYHAVISCGPMPMMEALADQCRGKTELVLSMESHMACGIGACL
                     GCTIQTVRGMERVCKEGPVFNAREVIFHG"
     gene            739..1641
                     /locus_tag="VFD79_06945"
     CDS             739..1641
                     /locus_tag="VFD79_06945"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HZH93822.1"
                     /translation="MARVYFCGIEFKNPVVAASGTFGFGREFQDYFDISSLGGISSKG
                     LTLNEKSGNKGRRIHETAAGLMNSIGLQNPGVEGFIKDEWPFMKGLDTVVMANLGGGT
                     VEEYVEGARLLDETDVPIIELNISCPNVKEGGMAFGIQCAPASIVVEAVRAVTDKPLV
                     VKLSPNAENIVEMALACVDAGADALSLVNTFNALAIDIKKRRPVFNNITAGLSGPAIK
                     PIALRMVYEVSRAVNVPIIGMGGIMNWEDAIEYIMAGATLVQVGSANFRKPDICLDII
                     RGIEEYMDGEGLEDLEEIRGIIGR"
     gene            1645..>2094
                     /gene="pyrE"
                     /locus_tag="VFD79_06950"
     CDS             1645..>2094
                     /gene="pyrE"
                     /locus_tag="VFD79_06950"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004832570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HZH93823.1"
                     /translation="MIIDLLRESEALLEGHFLLSSGRHSNRYVQCAKLLQYHDKAEKA
                     VGPIVEKVRDLDIDVVVGPAMGGIIVAYELGRQLGKPAFFTERDEGQMKLKRGFFIEK
                     GQRVLIAEDVVTTGKSSYEAIKVVEEYGGVVVGIAALIDRSSGDLDYT"
BASE COUNT          565 a          386 c          625 g          518 t
ORIGIN      
        1 ggatattggc tttgaaggat agctacaggg atggaaccat tattttaaat gaagaagttg
       61 caggtggagt attcaagctt gtccttgacg gggatttcac cggagctccc ggacagttct
      121 acatgctgag aggctggaat ggtctggatc cttttctgcc cagacccata agcatagggg
      181 atgttcagga tggaaggctc acccttctct atgaggtaag gggaaggggg acccatataa
      241 tttcaaaact ttcaccagga gacagcctaa gcgtcctcgg acccttggga aacggattcc
      301 agatctaccg ggaaggtagg attgccctgg tatcgggagg aattgggata gctccacttt
      361 tataccttgc caagaatacc gaggcggagg ttgaccttta ttcaggcttt agaagtgagc
      421 cttattttct tgatgagttc aagccctttg taaatgaaat caaggtgtcc actgaggatg
      481 gatcccttgg acaccagggc tttgttacag agctcttgag gcctgaagcc taccatgcag
      541 taatttcatg cgggccaatg cccatgatgg aggctcttgc agaccagtgc aggggcaaga
      601 cagaacttgt attgtcaatg gaaagccaca tggcctgtgg aataggggcc tgtcttggtt
      661 gtacaatcca aaccgtccgt gggatggaaa gggtatgcaa ggaggggcct gtgttcaatg
      721 ccagggaggt gattttccat ggctagggtg tatttctgtg gtatagagtt taagaatcct
      781 gtggttgctg catcgggaac ctttggattt ggaagggaat tccaggacta ctttgacata
      841 tcaagccttg gagggatatc ctcaaaagga cttaccctaa atgaaaagtc aggaaacaag
      901 ggtaggagaa tccatgagac agcagctggc cttatgaaca gcataggcct gcagaatccg
      961 ggagttgagg gatttataaa ggatgaatgg ccctttatga agggcctgga cacagtggtg
     1021 atggctaatc ttggtggagg aactgtggag gagtacgttg agggagcaag gctattggat
     1081 gaaaccgatg tgccaataat tgagcttaac atatcctgtc ccaatgtgaa ggagggtgga
     1141 atggcctttg gaatccagtg tgcgccggca tccattgttg tggaggctgt tagggctgtg
     1201 acagataaac cactggttgt taagctttca ccaaatgcag agaacattgt tgaaatggcc
     1261 cttgcctgtg tggatgcggg agcggatgca ctttccctgg tgaatacctt caatgccctg
     1321 gcaattgata taaaaaaaag aagaccggta ttcaacaata ttacagccgg gctttcagga
     1381 cctgcaatca agccaattgc actcaggatg gtatacgagg tatccagggc tgtgaatgtt
     1441 ccaataatag ggatgggagg aatcatgaac tgggaggatg ccatcgaata catcatggca
     1501 ggagcgaccc ttgttcaggt gggaagtgca aatttcagaa agcctgacat ttgtctggat
     1561 ataataaggg gtatagagga atatatggat ggtgaagggt tggaagacct ggaagagata
     1621 agagggatca tcgggaggta gattatgatt attgatttgt taagggaatc tgaagcacta
     1681 ctggagggac attttcttct gtcatccgga agacacagca acaggtatgt gcaatgcgcc
     1741 aagctcctgc agtatcatga caaggctgaa aaggccgtgg ggccaattgt tgagaaggtc
     1801 agggacctgg atattgatgt ggttgtggga cctgccatgg gtggaataat cgtagcctat
     1861 gagcttggca gacagcttgg aaagcctgcc ttcttcactg aaagggacga ggggcaaatg
     1921 aagcttaaaa ggggcttctt catagagaag ggtcagaggg tgcttattgc agaggatgtt
     1981 gttacaaccg ggaagtcctc ctatgaagca ataaaggttg tggaggaata cggtggggtt
     2041 gttgttggca tagccgccct tattgacagg agctcagggg acctggacta taca
//
LOCUS       DASRQF010000261         5562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_295545, whole genome shotgun sequence.
ACCESSION   DASRQF010000261 DASRQF010000000
VERSION     DASRQF010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295545"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VFD79_06955"
     CDS             complement(<1..556)
                     /locus_tag="VFD79_06955"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZH93824.1"
                     /translation="MTDSRKIAEMAEKLQDKLVQYRRQLHKNPELFMELPMTVKFVQE
                     KLADLGIESQNVGGGVVANIGNPGRGKTILLRADMDALPIEEETGLPFAAQGKAMHAC
                     GHDVHTSLLLGAAEILKSMEDEIPGNVKLMFQPGEEVLSGAKAMVEEGVLENPQVHGA
                     MMIHVFSGLDIEAGKVLVPKEGAAS"
     gene            complement(574..2115)
                     /locus_tag="VFD79_06960"
     CDS             complement(574..2115)
                     /locus_tag="VFD79_06960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HZH93825.1"
                     /translation="MLKQILHRYRELDQVRKRYLGFGAMLFLVFAIIVSVIFMGRIYS
                     LGETYAEYTRNEIINIKKDFLKDTVANTIQAVDKIRERQEGILQSKVNRIVGYLEIYP
                     DLELDRVISYLNEPDNKYLLDMVIEDNSGNIVFSSPQGLPSSDRIKGEEIIEFTREIQ
                     HGQYKISLFILRDTIKGQIEQELREKIYREIYFQEAYIWVNEVMDYAGGEGYAKRLIH
                     PNLRETEGSLLSTHMEDIAGNLPYLQELEGIRENGEIYFTYNFKKLESDEIGEKLTYA
                     KLYEDYDWIIAMGIYYDTIDAYYDSAREASEREVRKSMAVFLASGLVVLVLGMSWFLV
                     LEERYFRMSNMELKEELATDELTDAKSRRAGMQKLKEKFGKFSSSGGDCALLLMDLDD
                     FKHINDTYGHDAGDRILYQVVKSIKHSIRETDTIYRWGGEEFVILAEGLNPDTIQNFI
                     SKVLEAVKGVEIDFEDKKIGVTTSIGATFFREDDTSFEDGLKRADLALYESKKNGKGR
                     GTIRL"
     gene            complement(2232..3419)
                     /locus_tag="VFD79_06965"
     CDS             complement(2232..3419)
                     /locus_tag="VFD79_06965"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZH93826.1"
                     /translation="MISKEMYELGSKRSAIRDLFEYGKKQAEIVGSENVFDFSIGNPT
                     VPAPECVKEAIIDLLQTSSSSEIHGYTSAQGDINVRTGLADHMNNTYGTNLRSDNFYM
                     TCGAAASLTITLKALVADSEDEVIIIAPYFPEYKVFIKNAGAKVVELAADTKTFQIDL
                     EKLEKAITPKTKAIIINTPNNPSGVVYSEETIRSISALLNKKSEEHGEVIYLISDEPY
                     REIVYDGIKVPYVPKHYDNTIICYSYSKSLSLPGERIGYIMVPDSIVNAREVYAAVSG
                     AGRSQGYVCAPSMFQKVILKCMGKTADIDLYDSNRKLLYEGLTKMGYHCVKPEGAFYL
                     FVKALEEDSRSFAEEAKKLNLLIVAADDFGCPGYVRVSYCVDPEMIKRSLEAFQMLYD
                     VYR"
     gene            complement(3568..>5562)
                     /gene="ppdK"
                     /locus_tag="VFD79_06970"
     CDS             complement(3568..>5562)
                     /gene="ppdK"
                     /locus_tag="VFD79_06970"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HZH93827.1"
                     /translation="PRAVIYRRLHDIPSNLGTAVNVQSMVYGNMGDTSGTGVAFTRNP
                     ANGENKIYGEFLMNAQGEDVVAGIRTPRSISELEDIMPEVFEEFAKVTEILESHYKDM
                     QDIEFTIEKGKLFMLQTRTGKRTAQAAINVAVDLVKEGKISKEEAILRIEPNQLNQLL
                     HPTFDPKALGEATVIAKGLPASPGAASGKVYFHAKDVVEAKARGEKTLLVRQETSPED
                     IEGMVSAEGILTARGGMTSHAAVVARGMGKCCVAGCSEIRVDEDSKVIRAGDLTLHEG
                     DYLSLDGTTGTVYTGEIAKVQPELSGQFGEFMSWVDEVRIMKVRTNADTPRDAAAAIR
                     FGAEGIGLCRTEHMFFDEERIPAVRRMIVSTNESQRQKALNVLLPLQRKDFYEIYQAM
                     ESRPVTIRLLDPPLHEFLPSKNEDVESLAQSMGISFADLKDTINELAEVNPMLGHRGC
                     RLAVTYPEIAKMQTRAIIEAALDYKVKDGVEIVPEIMIPLIGDAGELAYVKKHVQDEI
                     DAVFAERKDSIKYLIGTMIEVPRAAITADEIAEEAEFFSFGTNDLTQMGFGFSRDDSG
                     KFLNEYVEKGIFDKSPFEVLDQKGIGKMMEMAVELGRKTREDIKLGICGEHGGEPSSV
                     EFCHRIGLDYVSCSPFRVPIARLAAAQAVLKEKMSIHIDK"
BASE COUNT         1345 a         1432 c         1026 g         1759 t
ORIGIN      
        1 ttgatgctgc accctccttg ggtaccaata cctttccagc ttctatatcc aaacctgaaa
       61 agacgtgtat catcatggct ccgtgaacct ggggattttc aaggaccccc tcctcgacca
      121 ttgccttggc acctgagagg acctcctcac caggctgaaa cataagctta acgtttcctg
      181 gtatttcatc ctccattgac ttcaatattt ctgccgcgcc aagcaataga ctggtatgga
      241 catcatggcc gcaggcatgc attgcctttc cctgggctgc aaatggcaag cctgtctctt
      301 cctcaattgg aagtgcgtcc atatccgccc taaggaggat tgttttcccc cttccgggat
      361 tcccgatatt ggcaacaact ccgcctccaa cattctggga ctctattccc aagtctgcca
      421 gtttttcctg aacaaacttc accgtcattg gcagctccat gaaaagttcc ggatttttat
      481 ggagttgtct tctgtattgg accagcttat cctggagctt ttctgccatt tctgctattt
      541 tccttgagtc tgtcataaat gcatctccct tgcctataat ctgatggttc ccctgccctt
      601 tccatttttc tttgattcat acagtgccag gtcagccctc ttcaagccat cctcaaagct
      661 ggtatcatcc tctctaaaaa atgttgcacc tattgatgtt gttacaccaa tttttttatc
      721 ctcaaaatct atctccacac ccttcaccgc ctcaagcacc ttggatatga agttttgtat
      781 cgtatctgga ttcagccctt cagccaggat gacaaattcc tccccacccc atctgtagat
      841 ggtgtcagtt tcacgtatgg aatgcttgat gctcttaacc acttggtaga ggatcctgtc
      901 tccagcatca tgaccgtagg tatcattaat atgcttgaaa tcatccaggt ccatcaagag
      961 gagggcacaa tctcctccgc tgctgctgaa ttttccgaac ttctccttta gtttctgcat
     1021 gcctgcacgt ctactttttg catctgtcaa ttcatctgta gccaattctt cctttagctc
     1081 catgtttgac atcctgaaat acctttcctc aagtacaagg aaccatgaca tgccaagaac
     1141 caatactaca agtccagaag caagaaaaac agccatggac ttccttacct ccctctcgct
     1201 ggcttctctt gcgctgtcat agtaagcatc tattgtatcg tagtagattc ccatggcaat
     1261 gatccagtca tagtcctcat ataatttggc ataggtcagc ttctctccaa tttcatctga
     1321 ttccagcttc ttgaaattat atgtgaagta gatttctcca ttttccctga ttccctcaag
     1381 ttcctgtagg tatggaaggt tcccagcaat atcctccata tgggtagaca ggagacttcc
     1441 ttctgtttcc ctgagattgg gatggatcag tcttttggca taaccctcac ctccagcata
     1501 atccatgacc tcattcaccc agatataggc ctcctggaag tatatctccc tatagatttt
     1561 ttcccttaac tcctgctcaa tctgaccctt tatggtatct ctgagaataa acagggatat
     1621 cttgtattga ccatgctgga tctctctcgt aaactcaatg atttcttccc ctttaatacg
     1681 gtctgacgag ggaagtcctt gcggacttga aaagacaata tttccggaat tgtcctcaat
     1741 aaccatgtcg agcaggtatt tgttgtcagg ttcattgaga tagctaatca ccctatccaa
     1801 ttccagatcc gggtaaattt ccaaatagcc gacaatacgg tttaccttgc tttggagtat
     1861 cccttcctgc ctttccctta tcttgtccac agcctgtata gtgttggcaa ctgtatcctt
     1921 gagaaagtcc ttctttatgt ttataatctc atttctggta tattcagcat aggtctctcc
     1981 caggctgtaa atcctaccca taaagattac cgagacgatg attgcaaaaa caaggaaaag
     2041 cattgcacca aaacccaggt agcgtttcct tacctggtcc agttccctgt atctatgtaa
     2101 aatctgcttt aacatggcat gtctccttat atttgaatta tgtcagtaat tatatcaaat
     2161 tcttctttgc tttgcaccct aaaagtctat tgttggtcaa aataataccc cggcaagccg
     2221 gggcattggt tctatctata gacatcatat agcatttgga aggcttccag ggacctcttg
     2281 atcatttcag gatcaacaca atatgaaacc ctaacatatc ctgggcatcc aaaatcgtca
     2341 gctgctacaa tcaaaaggtt cagcttcttg gcttcctccg caaagcttct tgaatcttcc
     2401 tccagggctt taacaaacag gtagaaagct ccttcaggct tgacgcaatg gtatcccatt
     2461 ttggtcaacc cttcgtaaag aagcttgcgg tttgagtcgt ataggtcaat atctgctgtt
     2521 tttcccatgc atttaaggat aaccttctgg aacatgctag gtgcacaaac atatccctga
     2581 gaacgtcctg ccccacttac cgctgcataa acctcccttg catttacaat gctgtctgga
     2641 accattatat agccgatcct ctctcccggg agagaaagtg acttgctgta ggagtagcag
     2701 atgattgtat tgtcgtaatg cttgggaaca tatggtacct ttattccatc gtatacaatt
     2761 tccctgtagg gttcatctga tatgaggtag atgacttcac catgctcctc tgacttctta
     2821 tttaacaggg ctgatatgga tcttatggtc tcctctgaat atactacccc agacggattg
     2881 ttcggtgtgt taattattat ggccttggtt ttgggcgtga tggccttttc aagcttttca
     2941 agatctatct ggaaggtctt tgtatctgca gccaactcaa caacctttgc accagcattt
     3001 tttataaata ccttatactc cggaaagtag ggtgcgatta tgataacctc atcctcagaa
     3061 tcagcgacca aggccttcag agtgattgtc aaggaagccg ctgcaccgca tgtcatgtaa
     3121 aaattgtcag accttagatt tgtaccgtat gtattgttca tatggtctgc cagtcctgtt
     3181 ctcacattaa tgtcaccctg tgcagatgta tacccatgaa tttcagaaga actggaagtt
     3241 tgaagcaggt caattattgc ttccttcaca cattcaggtg ctggtactgt tgggtttcca
     3301 atactgaaat caaatacatt ttcactaccc actatttctg cctgcttctt tccgtattca
     3361 aacaggtctc ttatggccga tctttttgaa cccagctcat acatttcctt tgatatcatt
     3421 cccatttcct cctctaatct ttataccatc actatgcttt tttatgttgc tagctttatt
     3481 ataccaggta aacgtttgca ccgccaatgt aaaatgaaat tatttcccca catgtaaggg
     3541 gctccggaaa accggagccc ctgggcttta cttgtcaata tgaatgctca tcttttcctt
     3601 gagaacagcc tgtgctgctg ccaatcttgc aattggaacc ctaaatggtg agcaggaaac
     3661 ataatccaag cctattctgt ggcagaactc aactgagcta ggctctcctc catgttcacc
     3721 acaaatacca agtttaatgt cttccctggt ctttcttcca agctccactg ccatttccat
     3781 catcttaccg atacccttct gatcaaggac ctcaaatgga ctcttatcga atattccctt
     3841 ctcaacatat tcattgagga atttccctga gtcatccctt gagaaaccaa atcccatctg
     3901 agtaaggtca tttgttccaa agctgaagaa ttctgcttct tcggcaatct catcagcagt
     3961 gattgctgcc cttggaacct ctatcatggt tcctatgagg tacttgatgg agtcttttct
     4021 ttcagcgaaa acagcatcaa tctcatcctg cacatgcttt ttaacatatg caagctctcc
     4081 tgcatctcca ataagtggaa tcattatttc aggtacaatt tccaccccat ccttgacctt
     4141 gtagtcaagg gcagcttcaa ttattgccct ggtttgcatt tttgctattt caggataggt
     4201 aactgccaga cgacatcccc tatggccaag cattgggtta acctctgcca gctcgttaat
     4261 ggtatcctta aggtctgcga aggagatacc cattgattga gccaggcttt caacatcctc
     4321 attcttgctt ggcaggaatt catgcagggg tggatccaaa agtctgattg taacaggtct
     4381 ggattccatc gcctggtaga tttcgtagaa atcctttctt tgcagtggca gaagtacatt
     4441 aagcgctttc tgtctttgac tttcgttggt ggaaactatc atcctcctta cggcaggtat
     4501 cctctcttca tcaaagaaca tgtgctctgt tctacaaaga ccgattcctt cagctccaaa
     4561 tcttatagcc gctgcagcat cccttggagt gtctgcgtta gttcttacct tcattattct
     4621 cacttcatca acccatgaca tgaattcacc aaattgacct gaaagctcag gctgaacctt
     4681 ggcaatttct cctgtgtaaa ccgtgccagt ggtaccgtcc agggataaat aatccccctc
     4741 atgcagggta aggtctccag ccctgataac ctttgagtcc tcatcaaccc taatttcact
     4801 gcaacctgca acgcagcact tgcccatacc tcttgcaaca acagcagcgt gcgatgtcat
     4861 tccgcccctt gcagtaagaa ttccctctgc gcttaccatt ccctcaatat cctctgggga
     4921 tgtctcctgt cttacaagta gggttttctc tccccgagcc tttgcttcca ctacatcctt
     4981 ggcatggaag tagactttac ctgatgcggc tccaggtgat gcaggaagtc ccttggcaat
     5041 aacagtagct tctcccaggg cttttggatc gaatgtggga tgaagcagct ggttgagttg
     5101 gtttggctca atccttagta tggcttcttc ctttgagatc ttgccttcct ttacaagatc
     5161 cactgctacg ttgattgctg cctgggctgt cctcttcccg gttcttgtct gtagcatgaa
     5221 aagctttcct ttttctattg taaactcaat gtcctgcata tccttgtagt ggctttcaag
     5281 gatttcagta acctttgcaa attcctcaaa tacttcaggc ataatgtctt ccagttctga
     5341 aatggatcga ggtgtcctaa tgcctgcaac aacatcctca ccctgagcgt tcataaggaa
     5401 ctctccgtag atcttgtttt ctccattggc tgggtttctg gtaaatgcaa cacctgtacc
     5461 ggatgtatcc cccatgtttc catataccat tgattgaacg tttacagctg tacctagatt
     5521 tgatggaatg tcatgaagtc ttctatatat tacagccctt gg
//
LOCUS       DASRQF010000262         8368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1738735, whole genome shotgun sequence.
ACCESSION   DASRQF010000262 DASRQF010000000
VERSION     DASRQF010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 8368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8368
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738735"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2413
                     /locus_tag="VFD79_06975"
     CDS             <1..2413
                     /locus_tag="VFD79_06975"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HZH93828.1"
                     /translation="ILEIVKEMDIDYSARKREQGALDYEDLQIQAYKLLLQDEILQKY
                     QEKFKYIMIDEFQDTNQLQKNIFYRLASGEEKLDRGNLFVVGDPKQSIYAFRGADIQV
                     FYDVVEDIESSHRGKVITLTENYRTVATVLDLVDKIFMGLLGERYTPLNPNKQVDQPV
                     EVEVLYNEEFSGTSKETQEYEADQIAKRIRYMVDKEDLHGNRKVRYKDIALLFRAMTR
                     SSIYEEALRKYNIPYTSTGGVGFYERDEILDLMNAIKVISNPRDMISMVGFLRGGLTG
                     LSDQAIFHLLEYCEREDGLQVIQGLKSLLEERKTDLDSLDYRIMEETARLLDRLAILK
                     DHLGLGDLLEVLLESTGFQESQLLMYQGMMRSRNVDAFVKLVRDYETTEGGSLEELID
                     FIEKRRKESEGDGVADQGGDSVSLMSIHKSKGLQFPVVIIPEMGRRGAYDRAIFQFDK
                     DMGLGIKNGDSSYYDLIYRNTKENLEEEVKRLIYVAMTRAEERLILGYQGKISGFKAL
                     IHQYLDGATLKLVSDFPVQPQEALMVKPILEPEGIVLKKTFPLLEFYQPPIAGRETYS
                     ISQYLKYVSCPREYFLTYKMRLSVGGDRPREESGEGLIDGATKGLLIHEFCRQYRKGE
                     DKAGILDQAAADLGVELKDDIISELDPLMDNFLAYLGGKPDYEVEMEKEFYVKLGNHV
                     VTGFIDRLEHHGDGKKIIDIKTNRLLNREGLVQLYRPQLIFYRWATGKILQTDKISSA
                     ILFLEDGSLVDVEASQAEENLLLQDMERVFDYVESHHQPDDYPLSRACSGSCKFNAFC
                     NREED"
     gene            2340..3227
                     /locus_tag="VFD79_06980"
     CDS             2340..3227
                     /locus_tag="VFD79_06980"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZH93829.1"
                     /translation="MTTPFPEPAAVVASLMLSAIGRKIDMKYMEMRDGYEIFYREDPV
                     EKAKGIIIMTHGFAEHLDRYDHVTDYLNRADYSVVRYDLRGHGRNRGRGNLDRFESYI
                     EDLEDILEGVRGANPQVPLITFGHSMGGLITAIFGSLHPSKCSAQILSGPALGRLPSA
                     EKLQPGIMKVLSRIAGGVMLKNPVGEGICGNRQVYLDYISDPLVLHKASIGLYYQFLF
                     RGVGLIGEVADKYTCPCLIVHGKEDPIVPIGISRWFYGEIPSQKKDLIEYDGLYHEIL
                     NEDKRDYILKDITDWLNKL"
     gene            complement(3224..4369)
                     /locus_tag="VFD79_06985"
     CDS             complement(3224..4369)
                     /locus_tag="VFD79_06985"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZH93830.1"
                     /translation="MNLTLMYATTFIIQFIIAMEMNFIGPLAPYLSEYFGIRDSQVIL
                     FNLGYSAVGILVPLFGVWADRYGNKKMISYSLLLFFTGSIISGFAPTALIFALGRILI
                     GLGYFSLSGSLMSYISEFIDYRNRGKASGILRTAFGIALLGSPIYTAFMTNHFGNLIS
                     IYLPVSVLAAIAFIMLRKLPETEILEESQLNLEEFIALLKHPRTVKVLVSLFLVISAP
                     VNMFNFLGIYLSSEFSLTQGQIGLSYSLIAAGTVLGILAATLFSDKIGKLTFSRIAFT
                     IMLFGLLPLPYVKSVVLIIGLAFAFAFGLDGGWTSYQAFSSEIQPGKRGTFLSLFYTI
                     NAATVTFYSLVGPLLYDLGGLRLTTAVGVGSSMIALVILFRLSRIPA"
     gene            4503..6224
                     /locus_tag="VFD79_06990"
     CDS             4503..6224
                     /locus_tag="VFD79_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZH93831.1"
                     /translation="MFKKFISYYKPHKTLFITDMFFAFLISVFDLVFPMFTRNMINVI
                     IPDGNMDLLIKWTGIMVLLFVLRYISQYIVAYYGHLLGVRIEHDMRRDIFTHLQTLPF
                     SYYDNTKTGHIMSRIINDLMDITELAHHGPEDVFISTIMLFGSFTILMFIEWRLTLII
                     FAFIPLMIWFAIKKRKRMEEAFMKARRKIASVNSQIENSISGARVAKSFTNEEYEIMK
                     FNDRNAKFASARGESYKVMAEFVSGVGIISSALNLIVISLGGYFVYRNIIDLGDLFAY
                     TLYVNFFMQPIKKLTDFMQQLQSGMTGFTRFQEVMSIEPSIVDREDAVELQNVEGRIE
                     FKNVTFSYGNDDEKVLSGINLTIDKGKTVALVGPSGAGKSTLCHLIPRFYETSSGEIL
                     LDGVNIKDVKLKSLRKNIGLVQQDVFLFTGTIRENIQYGNPSATEEDIVEAAKNASIH
                     DFIVSLPNGYDTYVGEKGVMLSGGQKQRISISRLFLKNPPVLILDEATSALDNETEQM
                     IQKSLERLSKNRTTLVIAHRLSTIKNADEILVMTGSGIEEKGSHQELLDKGGIYADLY
                     KSQFRDL"
     gene            6326..7261
                     /locus_tag="VFD79_06995"
     CDS             6326..7261
                     /locus_tag="VFD79_06995"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZH93832.1"
                     /translation="MKTRLGMALIASLIILTGFLVIPGIIDLSKAQEDRGQDIGDKGN
                     VLVEEGNSTEEALGNETENTSSSNDQTEHEEPTPEDDPVFLDEEEEVVEDLVPVIMDA
                     GKLIEEVKPTGKIAYLTFDDGPSEVVTPQILEILDRYGIKATFFVLGKMVDRNPDVLK
                     QTYENGHSIGNHTYSHNYEIIYRKTDNFLDEVYRTEKAIQIATEDSSYLTSLVRLPGG
                     SHAEYKKPTVVALEDEGFRVFDWNALNGDSELDKPTVDYLYGRFKETYKKQDKVVMLM
                     HDTDVKKKTVEALPSIIEHLLLEGYSFKTLEEYDH"
     gene            7277..7969
                     /locus_tag="VFD79_07000"
     CDS             7277..7969
                     /locus_tag="VFD79_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009344875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZH93833.1"
                     /translation="MSRKVLVVEDEESIRKFIKINLDRAGYTVLEAPTGEDGLDIASR
                     ETIDIVVLDVMLPGIDGFEVCRSLRSSHPHLGIIMLTAKSQDIDKIMGLEYGTDDYMT
                     KPFNPTELVLRIKSLERRLEKEGSSESSMLEFTPFKIDVYSRLFYRNNEEIELTPTEF
                     SIAKLFIENPGKAFKRDEILNHVWGVDFVGDSKIVDVNIRRLRSKIEEDSSQPYYIET
                     VWGVGYRWRAKS"
     gene            7979..>8368
                     /locus_tag="VFD79_07005"
     CDS             7979..>8368
                     /locus_tag="VFD79_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZH93834.1"
                     /translation="MRRSIKTRLIKNFMLIILVTVIFLEILLITGIRTHYYKNVEDIL
                     TSQIEFSTTFYARYFSSGNLEGILIEDTDIFWQHTAAQVQILDLEGNILMDSLGINFT
                     DKIETPDVQQAINLGRGVWTGNVEYDVE"
BASE COUNT         2590 a         1622 c         2087 g         2069 t
ORIGIN      
        1 tatccttgaa atagtcaagg agatggacat tgactattct gcaaggaaga gggaacaggg
       61 ggctcttgat tacgaggacc ttcagattca ggcatacaag ctcctcctgc aggatgaaat
      121 ccttcaaaag tatcaggaaa aatttaagta catcatgatt gatgaatttc aagacaccaa
      181 ccagctacag aagaacatct tttacaggct ggcctcagga gaggaaaagc tggatagggg
      241 aaacctcttt gtggttggtg atccaaagca gtcaatctat gctttcaggg gtgcggatat
      301 ccaggttttc tacgacgtgg tagaggatat tgaatcctca cacaggggca aggtcataac
      361 actcacagaa aactacagaa cagtggcaac agtccttgac ttggtggaca agatcttcat
      421 gggacttttg ggtgaaaggt acactcccct aaatccaaac aagcaggtgg accagcctgt
      481 ggaggtggag gttttgtaca atgaggaatt ttcaggtacc tccaaggaaa cacaggaata
      541 cgaagctgat cagatagcca agagaatcag atacatggtg gacaaggaag acctccatgg
      601 caacaggaaa gtccgctaca aggacatagc cttgcttttt agggccatga ccagaagctc
      661 aatctatgag gaagccttga gaaaatacaa tattccctat accagcaccg gtggagtggg
      721 cttctatgaa agggatgaaa tactggacct tatgaatgcc attaaggtga tcagcaatcc
      781 aagggacatg atatccatgg ttggattcct caggggaggt ttgacgggct tgtcagacca
      841 ggctatattc catctcctgg aatattgtga gagggaagat ggtctccagg taatccaggg
      901 gttgaaaagt cttttggagg aaaggaaaac agaccttgat agccttgatt acaggatcat
      961 ggaggaaact gcaaggcttt tggataggct tgccatcctc aaggaccact tgggactggg
     1021 agatcttctg gaagttcttt tggagtcaac aggatttcag gaaagtcagc tcctgatgta
     1081 tcaaggtatg atgaggtcta gaaatgtgga tgcatttgta aagcttgtca gggactatga
     1141 gaccactgaa ggggggagct tggaggagct aatcgacttt attgaaaaga ggagaaaaga
     1201 gtcagaggga gatggagtgg cagatcaagg cggtgattct gtgagtctta tgtccatcca
     1261 taaatccaag gggcttcagt ttcctgttgt tataatcccg gaaatgggaa gaagaggggc
     1321 ttatgacagg gcaatattcc agtttgacaa ggacatgggc ctgggaataa agaatggtga
     1381 ctccagctac tacgacctta tatacaggaa caccaaggag aacctggagg aagaggtaaa
     1441 aagactgata tacgttgcaa tgactagggc agaggagaga ttgatcctgg gttaccaagg
     1501 caaaatatca ggattcaaag ccttgatcca ccagtatctt gatggtgcca ccctcaagct
     1561 ggtatcggat tttccagtcc agccgcagga ggcccttatg gtcaagccga ttcttgagcc
     1621 tgaaggtatt gttctcaaga aaacatttcc attgttggaa ttctatcagc ctcccattgc
     1681 aggaagggag acctacagca taagtcagta tctaaaatat gtctcatgcc caagggagta
     1741 ttttctcacc tacaagatga ggctgtctgt tggtggtgac cggcccaggg aagaatctgg
     1801 ggaaggcctc atagatggtg caacaaaggg acttcttata catgagttct gcagacaata
     1861 cagaaagggt gaggacaagg cagggatact tgaccaggct gctgccgacc tgggagtgga
     1921 gctaaaggat gacataatca gtgagcttga ccccctaatg gacaattttt tagcatattt
     1981 aggtgggaaa ccggattatg aagtggaaat ggaaaaggaa ttctatgtga aacttggcaa
     2041 ccacgtggtc actgggttta ttgacaggct tgaacaccat ggggatggaa agaaaatcat
     2101 agacatcaag acaaacaggc tccttaacag ggagggactg gtccagctgt acagacccca
     2161 gttgatattc tacaggtggg ccacaggaaa gattctgcaa actgacaaaa tatcctcagc
     2221 aatcctcttc ctggaggatg gctcacttgt ggatgttgaa gcttcccagg cggaggaaaa
     2281 tctcctgctc caggatatgg agagagtctt tgactatgtg gaaagccatc accagccaga
     2341 tgactacccc ctttccagag cctgcagcgg tagttgcaag tttaatgctt tctgcaatag
     2401 ggaggaagat tgatatgaag tatatggaaa tgagagacgg atatgaaata ttctacaggg
     2461 aggatcccgt agagaaagcc aaggggatta ttataatgac ccatggattt gcggagcacc
     2521 ttgataggta tgaccatgtg acagattatc tcaacagggc tgactactca gtggtaaggt
     2581 atgatctgag gggacacggc agaaacaggg gaaggggtaa tctggacagg ttcgagtcct
     2641 atattgagga tcttgaggac atattggaag gggtaagggg ggcaaatcca caggttcccc
     2701 tgataacctt cggtcacagt atggggggac tgatcacagc catttttgga agcctgcatc
     2761 ccagcaagtg ttccgcacaa atcttgtcag ggcctgccct gggaaggctt ccctcggctg
     2821 agaagctgca gccgggaatc atgaaggtac tatccaggat agcaggtgga gtaatgctta
     2881 agaatcctgt aggtgagggt atttgtggaa acagacaggt atatcttgac tacatcagcg
     2941 atcccctggt actccacaag gcctccatcg gcctttatta tcagtttctt ttcagagggg
     3001 ttggcttgat tggggaagtg gcagacaaat acacctgtcc ctgccttatt gttcatggca
     3061 aggaggatcc cattgtaccg attggaatca gcagatggtt ttacggtgaa atcccctcgc
     3121 agaaaaaaga cctcattgag tatgatggcc tttaccatga aatactcaat gaggacaaaa
     3181 gggattatat tcttaaggat ataacagact ggctaaacaa gctctaggct ggaattcgac
     3241 tgagtctaaa taggatgacc aatgcaatca tggacgagcc tactccaaca gcagttgtca
     3301 gcctaagtcc tcccaggtcg tagagaagcg ggccaaccaa ggaatagaag gttacggttg
     3361 cagcatttat ggtatagaaa agactaagga aggtccctct ttttccaggc tgtatttcag
     3421 atgagaatgc ctgatatgat gtccagccac catcaagacc aaatgcgaat gcaaaggcaa
     3481 ggcctatgat caggaccaca cttttcacgt atggaagggg cagcaatccg aacaacatta
     3541 ttgtaaaggc aatcctagag aaggtcagct tgccaatctt gtctgaaaac aaggtggctg
     3601 caaggattcc aaggactgtc ccagctgcta tgagtgagta gctgagtcca atctgaccct
     3661 gagtaaggga aaactcagac gaaaggtaaa tcccaaggaa attgaacata ttaacgggtg
     3721 ctgaaatcac tagaaaaagt gaaaccagca ccttaactgt tcttggatgc ttcagtaggg
     3781 ctatgaactc ctcaagattc aactgacttt cctccaggat ctccgtttca ggaagctttc
     3841 tgagcattat gaatgcaatt gctgcaagta cgctgactgg caggtagatg cttatgaggt
     3901 tgccaaaatg gtttgtcata aatgctgtgt agattggact tccaagaagt gctattccaa
     3961 aggctgttct aaggatgccg ctggccttgc ccctatttct gtagtcgatg aactcagaaa
     4021 tgtaggacat aagacttcca ctcagggaga agtaaccaag accgattagt atcctaccca
     4081 gtgcaaatat cagtgcagtt ggggcaaatc cgcttattat acttcctgta aagaacagga
     4141 gaagtgagta tgagatcatt ttcttgttgc cgtacctatc cgcccatacg ccaaaaagtg
     4201 gcacaagtat tcccactgcc gagtatccca gattaaagag tatcacctga ctgtcacgta
     4261 ttccaaagta ctctgaaagg tagggagcca aggggccaat gaaattcatt tccatggcta
     4321 tgataaattg gatgataaaa gtagttgcat acataagtgt caggttcata gaagcctcca
     4381 tagtcatata ttaattaaag cacaaaatca ttctaaagca tgttataata ttcgtgtgtc
     4441 taagtattaa ccccatttat catatcacaa attcaaacta aaaagaagta gaggagctgc
     4501 ctatgttcaa gaaatttata tcctattaca aaccccataa aaccctgttt ataacagata
     4561 tgttctttgc ctttctaata tctgttttcg atctcgtatt tccaatgttc acaaggaaca
     4621 tgatcaatgt aatcattcca gatgggaaca tggacctgct tattaaatgg acaggaataa
     4681 tggtacttct atttgtccta agatatataa gccaatatat tgtcgcatac tacggacacc
     4741 tgcttggtgt caggattgag cacgacatga gaagggatat tttcacccat ctccagaccc
     4801 tgcccttcag ctactatgac aacactaaaa ccggccacat aatgtcaagg atcatcaacg
     4861 acctgatgga tataaccgag ttggcccacc atggtccgga ggatgtattc atatccacca
     4921 taatgctctt tggatccttc accatactta tgtttattga gtggagactg accctgataa
     4981 tctttgcatt cattccattg atgatttggt ttgccatcaa gaagagaaag aggatggaag
     5041 aggcctttat gaaggcaaga aggaagatag ccagtgtaaa ctcccaaata gagaacagca
     5101 tcagcggtgc aagagtagca aaatcattta caaatgaaga gtatgaaata atgaaattca
     5161 acgataggaa tgcaaagttc gccagtgcca gaggggaatc ctacaaggtt atggctgaat
     5221 ttgtttcagg agtggggatt atctccagcg ccctgaacct tattgtgatc tcactgggtg
     5281 gatattttgt ctaccgaaac ataatagacc tgggagacct gttcgcatat acactatatg
     5341 taaacttttt catgcagcca atcaagaagc tcacagactt catgcagcag ctgcaaagcg
     5401 gtatgaccgg tttcacaaga tttcaggagg tcatgtccat cgagccctca atagttgaca
     5461 gggaagatgc tgttgagctc cagaatgtag aaggaaggat tgagttcaag aatgtaacct
     5521 tcagctatgg aaacgatgat gagaaggtat tgtcaggtat taacctgaca attgacaagg
     5581 gaaagaccgt ggccctggtt ggaccttcag gtgctgggaa gtccacactc tgccacctga
     5641 taccaagatt ttatgagacc agctcaggag agattcttct ggatggggta aacatcaagg
     5701 atgtcaagtt aaaatcactc aggaaaaaca tcggcttggt ccagcaggat gtcttcctct
     5761 tcactggcac aataagggaa aacatccaat acggcaaccc ttctgcaaca gaggaggaca
     5821 ttgtagaggc tgccaaaaat gccagcatac atgatttcat agtgtccctg ccaaatggat
     5881 atgataccta tgtgggtgag aagggagtca tgctttcagg aggccagaaa cagagaatat
     5941 caatatccag gctcttcctg aagaatccac cggtactcat tcttgatgag gcaacctcgg
     6001 cactggataa tgaaactgaa cagatgattc agaagtccct ggaacggcta tcaaaaaaca
     6061 ggaccaccct tgtaatagcc cataggctat ccaccatcaa gaatgctgat gagattcttg
     6121 tcatgacggg aagtggaatt gaggagaagg gaagccacca ggaacttctg gataaggggg
     6181 gaatctatgc cgacctgtac aaatcacagt tcagggattt gtaatagtgt aatgcatttg
     6241 taaccgcttg ttaattgaat tgtaactact gtggtttgag ggtataataa tatgtaaaca
     6301 aagacacttt acaggagata tggacatgaa aacaaggctg ggtatggcac tgatagcatc
     6361 attaataata cttactggat ttctggtgat tcctggtatc attgacctgt caaaggcaca
     6421 ggaggaccgg ggccaggaca ttggtgacaa ggggaatgta ctggttgaag agggaaattc
     6481 aactgaggaa gctctgggaa atgagacaga gaatacctca agcagcaatg accagacaga
     6541 acatgaagaa cctacaccag aggatgaccc agtatttttg gatgaagagg aagaagttgt
     6601 ggaagatttg gtcccagtta tcatggatgc tggtaaattg atagaagaag tgaaacctac
     6661 agggaaaatt gcatacctta cctttgatga cggcccttca gaagttgtca ctccacaaat
     6721 actggagata ttggaccggt atggaataaa ggccacattc tttgtattgg gaaagatggt
     6781 ggacaggaac cctgatgttt tgaagcagac atatgaaaat gggcacagta ttggcaacca
     6841 cacctactct cacaactacg aaatcatata caggaagact gacaactttc ttgatgaagt
     6901 ttacaggacg gaaaaggcta ttcagattgc cacagaggat tcttcctatt tgaccagtct
     6961 ggtcaggctg cctggtggat cccatgcgga gtacaagaaa cccactgttg tggcccttga
     7021 agatgagggt tttagggttt ttgactggaa tgcattgaac ggcgactcgg agttggataa
     7081 acccacagtt gattatctat acggtagatt taaggaaacc tacaaaaagc aggacaaggt
     7141 tgtgatgctc atgcatgata cagatgtgaa aaagaagacg gtggaggcct tgccttcaat
     7201 aattgaacac ctgttgctgg aaggctacag cttcaaaacg ctggaggagt atgatcatta
     7261 gataaagggg gatattatga gtagaaaagt ccttgtagtt gaagatgaag aatccataag
     7321 aaaattcata aaaatcaatc tggacagggc cggctatact gtgttggagg cgccaaccgg
     7381 agaggatggg ttggatattg ccagcaggga gacaattgat atagttgttc ttgacgttat
     7441 gctgcctgga atcgacggtt ttgaggtatg caggtcccta aggtcaagtc acccacatct
     7501 gggaatcata atgcttacag ccaagagtca ggatattgac aagatcatgg gtttggagta
     7561 tggaaccgat gattatatga caaagccatt caaccccacc gaactggtcc tacggataaa
     7621 atcgcttgaa cgaaggttgg aaaaagaagg aagctcagag tcatccatgc ttgagttcac
     7681 ccctttcaag atcgatgtat actcaaggtt gttctacagg aataacgagg aaattgaact
     7741 gacacccact gaattttcaa tagcaaagct gtttatagag aatcctggga aggcatttaa
     7801 aagggatgaa atcctaaacc acgtttgggg tgtggatttt gtaggagact caaaaattgt
     7861 ggatgtaaac atcagaagac ttaggtccaa gatagaggag gactcaagtc aaccatacta
     7921 cattgaaacc gtatggggag ttggatatag atggagagcc aaaagttagg agcatctaat
     7981 gagaagaagc attaaaacca ggttaatcaa gaactttatg cttataatat tagttactgt
     8041 aatattcctg gagattctac taataacggg aatcagaacc cattactata aaaatgtgga
     8101 agacattctg acaagtcaga tcgagttttc tactactttt tatgcccgtt atttctcgtc
     8161 tggaaatctg gagggtatcc tgattgagga tacggacata ttttggcagc atactgctgc
     8221 acaggttcag atccttgatc ttgaggggaa catccttatg gactccctag ggataaactt
     8281 cactgacaag atagaaacac ctgatgttca gcaggccata aaccttggac gaggggtttg
     8341 gactggaaat gtggaatatg atgtggag
//
LOCUS       DASRQF010000263         7118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1062347, whole genome shotgun sequence.
ACCESSION   DASRQF010000263 DASRQF010000000
VERSION     DASRQF010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 7118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7118
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062347"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VFD79_07010"
     CDS             complement(<1..794)
                     /locus_tag="VFD79_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HZH93835.1"
                     /translation="MINVNNVSLRFGSQKLFDDVNLTFTPGNCYGVIGANGAGKSTFL
                     RILSGDVEADTGEISIAPNARMSVLKQDHYEFDDYGVIETVIMGNKRLYDIMIEKDAI
                     YAKEDFTDADGIKASELEGEFAEMDGWSAESEASSLLQGLGIPVSLHQKKVRDLTGNE
                     KVKVLLAQALFGKPGILILDEPTNDLDVKSIRWLQDFLGDFEGTVIVVSHDRHFLNEA
                     CTHMVDIDFGKIKMFVGNYDFWYESSQLALQLMRDQNKKKEDKIKDL"
     gene            complement(905..1330)
                     /locus_tag="VFD79_07015"
     CDS             complement(905..1330)
                     /locus_tag="VFD79_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93836.1"
                     /translation="MNTLLIILTAVAAGVTTYFIANKLNKGAVLASASVTLASGIAFP
                     HFFPTMGSTLMVVAACASYGGMVSRKNAPTLGDMVMISVIIGMLFTITASSYAGIGGR
                     LGTIAAISCFAWLGVKNSAKAASRARKSDFLSKSVEIWQ"
     gene            complement(1341..1700)
                     /locus_tag="VFD79_07020"
     CDS             complement(1341..1700)
                     /locus_tag="VFD79_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93837.1"
                     /translation="MTLDKTKSRILILAIVSTSLTWILNHQLGLGPIVANGLVGVAAA
                     IFMPGALAGIAYTSSFVGMSSLGVLPSVVFALIGGLIAGIVIIATSEIYAGIGGKGGT
                     TAAAAALVTRAISGLLG"
     gene            complement(1703..1876)
                     /locus_tag="VFD79_07025"
     CDS             complement(1703..1876)
                     /locus_tag="VFD79_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93838.1"
                     /translation="MTLKLLGLAAFAATGSGLLFTVGLHETGPLNTVILMAVGYFFTL
                     SLVDHYRELKEGE"
     gene            2254..2733
                     /locus_tag="VFD79_07030"
     CDS             2254..2733
                     /locus_tag="VFD79_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93839.1"
                     /translation="MFIYILKRVAMLVSIMLMLLGTVFYFSDTIKYEPMIQIIQEDYP
                     QYEFYSTKPEEYDDLPIGLDRIQAFHNLEYHLFNKENAINQPNIENEAIRGMDFNQVE
                     EFTERVGLLSGDIHDYYFPRAFFRYARGHYAVFLIVAAFETIRIMMAGDRERRRNVK"
     gene            2723..4066
                     /locus_tag="VFD79_07035"
     CDS             2723..4066
                     /locus_tag="VFD79_07035"
                     /inference="COORDINATES: protein motif:HMM:NF022641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HZH93840.1"
                     /translation="MSNRRRHKFEGFRMVDWRAMEDHYRDMASKGIFIEKFSWGISTY
                     REGYPEDREYSIAIYPHPKAFETVDDRMAKEYIQKQEEKGWEYVDSKENMHVFSKKPG
                     EDLHPIDKSDQIRNVKRGIKLESISFTLLALMNIFNIWRTYPIHPSILYTNTGILSLI
                     LMPMISLFLLVSVIENIKVWLSIRKSDDPGEYPYSNSSAVMLKRRMQSGSMLLIIILL
                     VVGVAFDSVMRGNMIIFTILPAIVGIFVAIKMRNFFTGREMGTVQKTLVAFAVVFIVI
                     GLITTINMRMISTGYGERLPEGYKALRLENPQETSFIREGSIIAPMRYTYTEWSWDDH
                     FETENHRFSTVEDRVSTEVTGYISDSVAEYLFQMELESYTRYVGEYWDAKEWYPEYDK
                     AYFATFREQEMDDGGTIFLKKDNYMVSLSMRRDLREDEVITEIDEFVDDLLKDRI"
     gene            4101..5918
                     /gene="pepF"
                     /locus_tag="VFD79_07040"
     CDS             4101..5918
                     /gene="pepF"
                     /locus_tag="VFD79_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005398875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HZH93841.1"
                     /translation="MAEKMPRRSEVDKSLTWDLTGIFRDEKSFKETFDKYISLGEGIE
                     REYKGRLKTAKDINDCLDRLRELEMLGGWLENYSYLNAEVDLTNMKAQEKLENISNRI
                     SAMESRLSFVEAEIMEASEDVLMEAARGNPENLGYYKFVIRKKPYRLHPEAERVLSAL
                     STTFEAPYRVYNMSKLADLSFEDFKAGGELYPLSFASFEGEWEYEKDTELRREAFRAF
                     SKDLSRYRNTMATAYQAHVTREKTLSDLRGFESVFESLLVDQEVSLDLYNRQIDIIME
                     HLAPHMRRYARLLGRIHGIDRMTFADLKVDVDPDFEPKVSIQETKEYIFDSLGIMGED
                     YLTMLQRAFDERWIDFAMNEGKSTGAFCSTPYGSHPFVLLSWTDKMRDVFTLAHELGH
                     AGHFHLAHRNQSIFNSEPSLYFIEAPSTMNEMLLTNHLMKTTDDKRMKRWVLSSMVSK
                     TYYHNFVTHLLEAHYQREVYRIIDGGGSVNADILDQLKRETLQEFWGEDVEITEGAEL
                     TWMRQPHYYMGLYPYTYSAGLTVSTEASQRILKEGDTAIEDWRNVLMAGGTKNPVELA
                     RMARVDITTDKPLLNTISYIGRMIDEIDKLTHELEKEDQ"
     gene            6013..>7118
                     /locus_tag="VFD79_07045"
     CDS             6013..>7118
                     /locus_tag="VFD79_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HZH93842.1"
                     /translation="MEYLSLENLINRVGLILILAFLLSRLGMFRKLVSKRKISLLDKL
                     VLALIFGAFGIIGTYTGIHIQGAIANSRVIGVFVGGLLGGPFVGILSGIIAGGHRYLM
                     DIGGFTALACAVSTISEGVMAGLLKKRFDRSDHRFTFALLAGFAAEVLQMVIILIMAK
                     PYGDALELVEIIGIPMILANGIGIAMFIAMVDSVLHGMEKEASYQAQLALKIANRTMF
                     FRDGFNEESARKTAEIIKEMTNVAAVAFTDREQIIAHVGQGEDHHRPGTGVMTDLTRK
                     ALNMDSFTIADSKEDIGCDHKGCRLRSAIIVPIKQENRVIGALKLYKAEDRAITMVET
                     ELALGLAQLFSTQLELSRVDHQRELLAKSELKAL"
BASE COUNT         2123 a         1428 c         1698 g         1869 t
ORIGIN      
        1 agatccttga tcttgtcttc cttcttcttg ttctggtccc tcatcagctg caatgcaagc
       61 tggcttgact cataccagaa gtcgtagttt ccaacaaaca tttttatctt tccaaaatct
      121 atgtcaacca tgtgggtaca ggcttcatta aggaagtgac ggtcgtggga aacaacaatg
      181 acagttccct cgaaatcccc taaaaagtcc tgcagccatc tgatgctttt gacatccagg
      241 tcgtttgtag gctcgtcaag tattaggatt ccgggctttc caaagagagc ctgtgccaga
      301 aggaccttta ccttttcgtt accagtaagg tcacgaacct tcttctggtg aagggaaaca
      361 ggaattccaa gtccctgcag cagtgaagaa gcttcacttt ccgcactcca accgtccatc
      421 tcagcaaatt caccctccag ctcagaagcc tttatgccgt cggcatccgt gaagtcttcc
      481 tttgcataaa tcgcgtcctt ttcaatcatt atgtcgtaga gtcttttatt ccccatgata
      541 accgtttcga ttactccata gtcatcaaac tcgtagtggt cctgttttaa gacactcata
      601 cgggcatttg gtgctatgct tatctctccg gtatcagcct cgacatcacc ggataggatc
      661 cttaggaatg tactcttacc tgcaccgttt gccccaatga caccatagca gttgccggga
      721 gtgaaggtca ggtttacatc gtcaaatagt ttttgggagc caaatctgag gctcacgtta
      781 tttacattaa tcaattgttc ttcatcctct tttcaatatt ttcatcaagt cattgtacca
      841 tgagtgactc tttaattcaa caaaaaagaa ccttttcagg ttccttaaga tgttttttca
      901 agtgttattg ccaaatctcc acggatttcg atagaaaatc acttttccga gcccttgatg
      961 cagcctttgc ggagttctta acacccagcc aggcaaagca ggaaattgca gcaatggttc
     1021 caagccttcc accgattcct gcataggatg aggcggttat agtaaagagc attccaatga
     1081 ttactgaaat catcaccatg tcacccaatg taggggcgtt ctttctggaa accattccgc
     1141 cataggaagc gcaggctgca accaccataa gcgttgagcc catggttgga aagaaatggg
     1201 gaaaagcaat ccctgaagcg agggttacgg atgctgatgc aagtacagct cccttgttta
     1261 gcttgttggc tatgaaatag gttgttacac ctgctgcaac ggcagttaat ataatcagta
     1321 gagtattcat ggatttcctc ctagcctaat agtcctgaaa ttgcccttgt gacaagcgct
     1381 gcggcagcgg ctgtggttcc gcccttgcca ccgattcccg cgtaaatttc agatgttgcg
     1441 ataattacaa tccccgctat caatccaccg ataagtgcaa acacaactga cggaagtaca
     1501 cccaaagagc tcattccaac aaaggacgag gtgtatgcta taccagccag ggctcctggc
     1561 ataaatatgg cagcagcaac tccaacaagt ccgtttgcca ctattggacc tagtccaagt
     1621 tgatggttaa ggatccaggt aagggaagtg cttacaattg ccaatatgag tattctggac
     1681 tttgtcttgt caagagtcat ggctattcac cttcctttag ttctctgtag tggtctacaa
     1741 gacttagggt aaaaaagtaa ccgaccgcca ttaatattac tgtatttagt ggacccgttt
     1801 catggagtcc cactgtaaat aatagtcctg aacctgtagc agcaaatgct gcaagtccca
     1861 gcaatttaag tgtcataata aaacctccaa aacattgtta ttaatataac ggaatcttat
     1921 catggcatga caaataaagc aatagtattt tgaaaaaaac attgagtatt tttcaaaaat
     1981 agaaggcgta attttctaat aattactaaa atagtggagc ataggtcaaa taataaagaa
     2041 atattcataa atttaacagt ttcaatttta aagttaaata tataacaaac atatgaccat
     2101 ttaaagtctt gtggataaac ctgaccatct tttttccgcg tatgtacaaa catgtaaaat
     2161 cagccaatta ttttggaaaa atagcaagaa tttactaaaa ccacatcaaa ttcatgttat
     2221 aattaagtgg agtacaaatg aaaggggatt cctatgttca tatatatact gaaaagagtt
     2281 gccatgctgg tttcaataat gcttatgcta ttgggtactg ttttctattt ttccgatacc
     2341 ataaaatacg aaccaatgat tcagattatt caggaggatt atccacagta tgaattctat
     2401 tccactaagc cagaggaata tgatgacctg ccgattggac tggatagaat acaggcattt
     2461 cacaatcttg aataccattt gttcaataag gaaaatgcca ttaaccaacc taatatagaa
     2521 aatgaagcca tcagaggcat ggatttcaac caagttgaag aatttactga aagggttgga
     2581 ctcctttctg gtgatatcca cgactattat tttcctagag catttttcag gtacgcaagg
     2641 ggacattatg ctgtctttct tattgttgct gcatttgaga ccatcaggat aatgatggct
     2701 ggagataggg aaaggaggag aaatgtcaaa taggagaaga cacaagtttg aaggcttcag
     2761 gatggtggac tggagggcaa tggaggacca ctacagggac atggccagca aggggatctt
     2821 tatagaaaag ttctcttggg ggattagcac ctatagggaa ggatacccag aggataggga
     2881 gtattcaata gccatatatc cacaccccaa agcctttgaa acagttgatg acagaatggc
     2941 caaggagtac atacaaaagc aggaggaaaa gggctgggag tatgtggatt ccaaggaaaa
     3001 catgcatgtt ttcagcaaga agccgggaga ggacctccac ccaatagaca aatcagacca
     3061 aatccggaat gttaaaaggg gtatcaagct tgagagcata agctttactc tccttgccct
     3121 tatgaatatt tttaatattt ggcggacata tccaatacat ccatcgattc tctatacaaa
     3181 tactgggata ttaagtctga ttttaatgcc catgatatca ttgttcctgt tggtatcagt
     3241 cattgaaaac attaaggtat ggctgagcat aaggaaaagt gatgacccgg gagagtatcc
     3301 gtattcaaat tcatctgcag ttatgctgaa aagaaggatg cagagcggaa gtatgctcct
     3361 gataatcatt ttactggtgg ttggagtggc ttttgattca gtcatgagag gaaacatgat
     3421 aatatttacc atactgccgg ctattgtggg gatctttgtt gcaattaaaa tgaggaactt
     3481 cttcactgga agggagatgg ggacagtcca gaagaccctg gtggcatttg ctgttgtatt
     3541 tattgttatt ggcttgataa caacaataaa tatgaggatg atttcaacag gctatgggga
     3601 aaggcttcca gagggctaca aggccctgag attggagaac ccacaggaga catccttcat
     3661 aagggagggt agcataatag cacccatgag gtacacctat accgaatgga gctgggatga
     3721 ccattttgaa acagagaacc accgtttttc tacagtggaa gacagggttt caacagaggt
     3781 aacagggtat ataagtgata gtgttgcaga atacctgttc caaatggagc ttgaaagcta
     3841 cacaagatat gttggggaat actgggatgc caaggagtgg taccctgaat atgacaaggc
     3901 ctactttgca accttcagag aacaggagat ggatgatgga gggacgatct tccttaagaa
     3961 ggacaattac atggtaagtc tttcaatgag aagggacctg agagaggatg aagtaattac
     4021 ggagatagat gagtttgtgg atgacctgct taaggatagg atctagtctt gggtcaataa
     4081 tttgaacggg gggaatggtc atggctgaga aaatgccaag gagaagcgag gttgacaaga
     4141 gtcttacatg ggatctgact ggaatattca gggatgagaa gtccttcaag gagacctttg
     4201 acaagtacat ctccttaggt gaaggaattg agagggaata caagggtagg ctaaagacag
     4261 ccaaggatat caatgattgc ctggaccgat tgcgtgaact ggagatgctt gggggctggc
     4321 ttgaaaacta ttcctacctg aatgcagagg ttgacctgac aaacatgaag gctcaggaaa
     4381 agcttgagaa cataagcaat aggatttctg ccatggaaag cagacttagc tttgttgagg
     4441 cagaaataat ggaagcttcc gaggatgtgc ttatggaagc tgcaagggga aaccctgaga
     4501 acctgggtta ttacaagttt gtaataagga aaaagccata caggctccac cccgaagctg
     4561 aaagggtcct ttcggccttg tccacaacct ttgaggctcc ctacagggtg tacaacatgt
     4621 caaagcttgc agacctttcc tttgaagact tcaaggcggg gggagaacta tatcctttaa
     4681 gttttgcatc ctttgaagga gaatgggagt atgaaaagga tactgaactt agaagagagg
     4741 cctttagggc tttctcaaag gacttgtccc gctacaggaa caccatggca actgcctacc
     4801 aggcccatgt aacaagggag aagaccctgt ctgacctgag ggggtttgaa tcggtatttg
     4861 aaagccttct ggttgaccag gaggtaagcc ttgaccttta caacaggcag atagatatca
     4921 taatggaaca tcttgcaccc cacatgagaa ggtatgcaag gctccttgga agaatccacg
     4981 gaattgacag gatgaccttt gcagacctta aggttgatgt ggatcctgat tttgaaccta
     5041 aggtctccat ccaggagacc aaggaatata tattcgacag ccttggaata atgggtgagg
     5101 actacctgac catgctccaa cgggcgttcg atgaaagatg gattgatttt gccatgaacg
     5161 agggtaaatc aactggggca ttctgttcaa ccccatacgg gtcacatcct tttgtactcc
     5221 tgtcctggac cgacaagatg agggatgttt tcacactggc ccatgagctg gggcatgccg
     5281 gacatttcca tctggcccac aggaaccaga gcattttcaa ttctgagcca tcactctact
     5341 ttattgaggc accatcaacc atgaatgaga tgctcctaac aaaccacctt atgaagacca
     5401 ctgatgacaa aaggatgaag aggtgggttc tatcatccat ggtttcaaag acctactacc
     5461 acaactttgt cacccacctg ttggaagctc actaccaaag ggaggtctac aggataatag
     5521 acggcggggg aagtgtaaat gcagatatcc ttgaccagct taagagggag accctgcagg
     5581 aattctgggg tgaggatgtt gaaataacag agggagctga gcttacctgg atgaggcagc
     5641 cccactacta catgggacta tacccataca cctattctgc aggactgacc gtttcaactg
     5701 aagcaagcca gagaatcctc aaggaggggg acactgccat agaggactgg agaaatgttc
     5761 ttatggctgg aggaaccaag aatccggttg aactggcaag gatggcccgg gtggatatca
     5821 ctaccgataa gcccttgcta aacactattt catacattgg caggatgatc gatgagatcg
     5881 ataagctgac ccatgaattg gagaaggaag atcagtgaaa ccggacccct ggagacaggg
     5941 gtccgaattt taccttgatt tactggagta aaatatatat actgttgatg aattcagaat
     6001 tgaggtgttc aaatggaata tttaagtttg gaaaatctga taaacagggt tgggctgata
     6061 ctgattctgg cattcctcct atccaggttg ggaatgttca gaaagctggt ctcaaagaga
     6121 aagataagtc ttttggacaa gttggttttg gcccttatat ttggggcttt tggaatcata
     6181 ggtacatata cagggataca tattcagggt gcaatcgcaa attccagggt aattggtgtc
     6241 tttgtgggag gccttttggg gggccctttt gttgggatcc tttcagggat aatagctgga
     6301 ggacacaggt atcttatgga tataggaggt ttcacagcac ttgcctgtgc agtttcaaca
     6361 atatcagaag gagtaatggc tggacttctg aaaaagaggt ttgaccgttc ggaccacagg
     6421 tttacttttg cacttttggc gggatttgct gcagaggttc tgcagatggt gattattctc
     6481 atcatggcca agccctatgg tgatgcactt gagcttgttg aaatcatagg aatcccaatg
     6541 atacttgcca atggcatagg aatagcaatg ttcattgcca tggttgacag tgtactccac
     6601 ggaatggaga aggaggcatc ctaccaggca caactggcac ttaaaattgc aaataggaca
     6661 atgtttttca gggatggctt caacgaagag tctgccagaa aaactgcaga gataatcaag
     6721 gaaatgacca atgtagcagc tgtggctttt acggataggg aacaaataat tgcccatgtg
     6781 gggcagggag aggaccacca cagaccaggt acaggtgtca tgacagactt gactaggaaa
     6841 gcccttaaca tggatagctt caccatagcc gacagcaaag aggacatagg ctgtgaccat
     6901 aaggggtgta ggctaaggtc tgcaataata gttcccatta agcaggagaa tcgggttatt
     6961 ggagcactta agctttacaa ggctgaggac cgtgcaataa caatggttga aactgagctt
     7021 gcactggggc ttgcccagct cttctccacc cagcttgaac ttagccgtgt tgaccatcag
     7081 agggagctcc tggccaagtc ggaactgaag gctctcca
//
LOCUS       DASRQF010000264         2765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_656898, whole genome shotgun sequence.
ACCESSION   DASRQF010000264 DASRQF010000000
VERSION     DASRQF010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656898"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1278)
                     /locus_tag="VFD79_07050"
     CDS             complement(<1..1278)
                     /locus_tag="VFD79_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZH93843.1"
                     /translation="MSDDMKRNLGVKSLNFKLWTYFMIFAAIIMVILWLLQIVFINSF
                     YKTMKTNEIKKIGNELVSLYGTASFDQELLNKSFAEGIVIHILDQRGSVVFPLDIVDL
                     VRRPRLQESAFSEFLNQLYMAEEGYTVYTRGHENLDAETIVYGGILENRDGPNYFLFI
                     NTILEPIDSTVNVLKNQLMIITLIALTLSMGLSLFLSRRLSEPIASITKSAKGLGEGR
                     YDVVFKKGDYLEIDNLADTLDQATKELKKSEDLRRDFIANVTHDLKTPLTVIKSYGEM
                     VRDISGENPEKRNDHMNTIIQEADKLGKLVDDMLDLTKIQSELRELKMEEMDLLSEAE
                     EVVRRFSYFMDHEGYEIRVSSSGDTRVKGDRRKLSQAVYNLVNNGINYSLDDKRIIIQ
                     VHGQEDHVRFSIRDFGQGIEEGELDYIWDRYYRC"
     gene            complement(1271..1945)
                     /locus_tag="VFD79_07055"
     CDS             complement(1271..1945)
                     /locus_tag="VFD79_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZH93844.1"
                     /translation="MYRILVVDDEDKIREVVREYGEFNGHKVYEAQDGIQAVNMCRED
                     EFSIIVMDIMMPRLDGFSAVKEIRKFSNTPVLMLSARGEEYDKLFGFELGIDDYVTKP
                     FSPKELMARINAIISRQKGRESRDQVEFNGLRIDRDGRNVYVDDRKVDMTPKEYELLV
                     YMIQNKNRALSREQLLRDIWGYDFYGDDRTIDTHIKMLRSSLGPYRDYIVTMRGLGYK
                     FEFGNE"
     gene            complement(2104..>2765)
                     /locus_tag="VFD79_07060"
     CDS             complement(2104..>2765)
                     /locus_tag="VFD79_07060"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HZH93845.1"
                     /translation="ERYELTEEVLEGFIRNLIQGKGEDAKEIAKALHGGGMRLTEIIT
                     NLFIPTLHRLGTMWEKEEITVAQEHYSTGIISSLTEFLSEVEPTQGDKRYVALFMTPG
                     DEEHTLSLRFVSQFFKEAGWRIIFLGRSIPLGDLKQVIKQGKVDLVVLSVFSTGGLNS
                     AEYLIEALKKEGGKRVPRILMGGRGLDSQETADKIGVDIYLDRLEGLEGMIEEIEEMI
                     K"
BASE COUNT          674 a          709 c          495 g          887 t
ORIGIN      
        1 acatctgtag tatctatccc agatataatc caactcgccc tcttcaattc cctgtccaaa
       61 gtccctaatt gaaaatctga catggtcctc ctgtccgtgg acctggatga tgatccgttt
      121 gtcatcaagt gaatagttga ttccgttgtt gaccagattg tatacagcct gtgaaagctt
      181 gcgcctgtca cctttaaccc ttgtgtcacc gcttgaactt accctaatct cgtagccttc
      241 atggtccata aagtagctga accggcggac tacctcctca gcctcagata ggaggtccat
      301 ctcctccatc ttaagctccc ttagctctga ctgtatcttg gtcaggtcaa gcatatcatc
      361 taccaatttg ccaagtttat cagcctcttg tattattgtg ttcatatggt catttctttt
      421 ttccggattc tcgccggaaa tatccctgac catttcacca taggacttga tcactgtcag
      481 tggagtctta aggtcatggg taacatttgc aatgaagtcc cttctaaggt cctcgctttt
      541 ctttagctcc tttgttgcct ggtccagagt atcggccaga ttgtcaatct caagatagtc
      601 acccttttta aaaaccacat cataccttcc ctcacccaga ccctttgcgc tctttgttat
      661 gcttgcaatt ggctctgata gtcttcttga aagaaagagg gacagcccca ttgaaagggt
      721 cagagcgatc aaagttatga tcatcagctg gtttttcaat acattgactg ttgaatcaat
      781 tggttcaagg atagtgttta tgaacaggaa gtaatttgga ccatccctgt tttcaagtat
      841 tcctccatac acaatggtct cggcatccag gttctcatgc cctctggtgt agactgtgta
      901 cccttcctct gccatgtata gctgattcaa gaattccgaa aaggcagact cctgaagtcg
      961 cggtcttcta acaaggtcca ctatatccaa agggaaaaca acacttcccc tttgatcaag
     1021 aatatgaatg acaatacctt cagcaaagga tttgttcaaa agctcctggt caaaactcgc
     1081 tgttccgtat agtgaaacca gctcattgcc gatcttcttg atttcattgg tcttcatggt
     1141 cttgtaaaag ctatttataa agacaatctg aagaagccac aatataacca ttattattgc
     1201 ggcaaaaatc atgaagtatg tccatagctt aaaattgaga gactttactc ccaggtttct
     1261 tttcatatca tcactcattt ccaaactcaa acttgtatcc taggcccctc atggtcacta
     1321 tatagtccct gtagggtcca agactgcttc taagcatctt tatatgggtg tctatggtcc
     1381 tgtcatcacc atagaagtca tagccccaga tatcccgcag aagctgttcc cggcttagtg
     1441 ccctgttttt attctggatc atatatacaa gcagttcata ctccttggga gtcatgtcaa
     1501 ccttcctgtc atcaacatag acatttctcc catcacggtc tatccttagt ccattaaact
     1561 ccacctggtc tctactttct cttccttttt gacggcttat tatggcattg attctggcca
     1621 tcagttcttt gggtgagaag ggctttgtca cgtaatcgtc aattccaagc tcaaagccaa
     1681 agagcttgtc gtactcctca ccccttgcgg atagcatcag aacgggggtg ttggaaaatt
     1741 ttcttatctc ctttacagcc gaaaacccat caagccttgg catcattata tccatgacaa
     1801 ttatgctgaa ttcatcctcc ctgcacatat tcacagcctg tattccatcc tgggcctcat
     1861 agaccttgtg accgttgaat tcaccgtatt cccttactac ctccctgatt ttatcttcat
     1921 catcaacaac aaggattctg tacatttaac cacctcttgt ttcattttat tacacttttg
     1981 tgaagtccat gtgaaatctt gtcttttact gtctgcaaga tatttaatat tggacatgat
     2041 ctcttcaatt aaataatagc aaatcctcaa taaagaaaca acagactcat aaatcgagcc
     2101 tgtttatttg atcatctctt caatttcttc aatcattcct tctaaccctt ccagtcggtc
     2161 aaggtagatg tcaactccga tcttgtccgc cgtttcctgg gaatccaaac cacgtcctcc
     2221 cataaggatc cttggtactc ttttgccgcc ttcctttttc aaggcctcta tcaggtactc
     2281 tgcactgttc aaaccacctg ttgagaatac tgagagcact actaggtcaa cctttccctg
     2341 tttgattacc tgcttcaggt ctcccaaggg tatgctcctt ccaaggaaga ttatcctcca
     2401 gcctgcctcc ttgaagaact gggacacaaa ccttaggctt agggtgtgct cctcatcacc
     2461 tggggtcatg aaaagtgcca cgtatctttt gtcaccttgg gtgggttcga cctcagatag
     2521 aaattcggtc aaggatgata ttatgcctgt cgagtagtgc tcctgggcaa ctgttatctc
     2581 ctctttttcc cacattgttc caagtctgtg gagggttggt atgaagaggt tggttatgat
     2641 ttctgtaagt ctcatgcctc caccgtggag ggcttttgct atttcctttg catcctcacc
     2701 ctttccctgg atcaggttcc ttatgaaacc ctccaatact tcttccgtca attcgtatct
     2761 ctcgg
//
LOCUS       DASRQF010000265         2236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_758374, whole genome shotgun sequence.
ACCESSION   DASRQF010000265 DASRQF010000000
VERSION     DASRQF010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758374"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VFD79_07065"
     CDS             <1..551
                     /locus_tag="VFD79_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZH93846.1"
                     /translation="LSLQLDDRGFIKTDDKMRTSIKNVYAIGDVNGKLPLAHVASAEG
                     ITAVENILGEESILNYNNIPRVVYSFPELASVGITEDEAKEKGVDYSVGKFPLSANGM
                     AIAEDETTGFVKIISDNQYGEVIGVHMAASVASDMISKAVAVMNLEGTVYDLAKTVHP
                     HPTFAETIMEAAFASIDKPIHM"
     gene            complement(599..1723)
                     /gene="rsgA"
                     /locus_tag="VFD79_07070"
     CDS             complement(599..1723)
                     /gene="rsgA"
                     /locus_tag="VFD79_07070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HZH93847.1"
                     /translation="MAGSRERIETKGVQMDKLNIEKLGYDKFYEENYLKAREMDEGLV
                     AGRVTAVHRERYQVITEYGEGWATLKGSIFYKGDQTVEYPTTGDFVAIRHNPDGEDII
                     YKVLERKSEFSRMDSFRRTKQVVAANFDYVLILSSMNEDFNLNRLDRYIVSAWESGAE
                     PLVVLTKADISKDPEFYIRSVSDNAPGVQIFAVSSITGEGIPALRTYLKRESTLVLLG
                     SSGVGKSTLVNALAGTEVMKVNDIREDDSKGKHTTTHRQLIVMDDGTMVIDTPGMREL
                     ELWSSDSGMEETFHDIETIAEGCRFRDCSHRDEPGCAITEALRTGEISEKRWNNYQKL
                     KREAEFARTKEEQSARVQKKLQDRKFGKMLKDHKKKKSIY"
     gene            complement(2011..>2236)
                     /locus_tag="VFD79_07075"
     CDS             complement(2011..>2236)
                     /locus_tag="VFD79_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93848.1"
                     /translation="KVLMEHNVDEGIFSSITKILNEGKPYNKTLISVIKDKEVLDKAV
                     RNIKEILNMKEKKAAGFMFVLPVLEIHEGL"
BASE COUNT          557 a          559 c          401 g          719 t
ORIGIN      
        1 atctcagcct tcagcttgac gacagaggct ttatcaagac tgatgataaa atgaggacca
       61 gtatcaagaa cgtatatgcc ataggtgatg tcaacggcaa gctccccctt gcccatgtgg
      121 cgtccgctga aggaatcaca gcagttgaga atattctcgg tgaggaatca atcctcaatt
      181 acaacaatat cccaagggta gtctactcct tccctgaact tgccagcgtt ggaataaccg
      241 aggatgaagc caaggaaaag ggtgttgact actcagttgg aaagtttccc ctatccgcca
      301 atggaatggc aattgccgag gacgaaacaa caggctttgt gaagataatc tccgataatc
      361 aatatggcga ggtgattggg gtccacatgg ccgccagcgt tgcctctgac atgatttcaa
      421 aggctgttgc agttatgaat cttgaaggta cggtttatga ccttgccaaa acggtccatc
      481 cacatccgac atttgccgaa acaataatgg aggcggcttt tgcatcaatt gacaagccta
      541 tccatatgta atctttaaat ttaaatatgc tgtgtgacca gtcacacagc atttttttct
      601 aatatatgct tttctttttc ttgtggtcct taagcatttt accgaatttt ctatcctgaa
      661 gctttttttg cacacgggca gactgctctt ccttggttct tgcaaattca gcctccctct
      721 taagcttctg gtagttattc catctctttt ctgagatttc cccggtgcgg agtgcttccg
      781 taatggcgca gccaggttcg tccctatggc tgcaatctct gaatctacag ccctctgcta
      841 ttgtttcaat atcatggaag gtttcttcca ttcctgagtc tgagctccat agctcaagtt
      901 ccctcatgcc tggtgtatct atgaccatgg ttccgtcatc catgacaatc agctgcctgt
      961 gtgttgttgt atgcttaccc tttgagtcat cttcccttat atcgttgacc ttcataactt
     1021 ctgttcctgc cagggcattt acaagcgttg attttcccac tcccgatgac cccagcaata
     1081 ccagtgtgga ttcccttttc aggtaagtcc tcaatgcagg aatcccttct ccagtaattg
     1141 agctaacagc aaaaatctgc acccctggtg cattgtcact gactgatcgg atgtaaaact
     1201 ccggatcctt agaaatatca gcctttgtca aaaccaccag aggttctgca ccactttccc
     1261 aggcggaaac aatatacctg tcgagcctgt tgagattgaa gtcctcattc atggaggaca
     1321 gtatcaacac atagtcaaaa tttgctgcta cgacctgctt ggtccttcta aaggaatcca
     1381 tccttgaaaa ctcgcttttt ctctctaaga ccttgtagat aatgtcctct ccatcaggat
     1441 tatgccttat ggccacaaaa tcaccagtcg ttgggtattc cactgtctgg tctcccttgt
     1501 agaatattga acctttcaag gttgcccagc cttccccata ctctgtgatt acctggtatc
     1561 tttccctatg gactgcagtc actcttccag ctacaagccc ctcatccatt tcccttgcct
     1621 taagataatt ttcttcatag aatttatcat accccagctt ttcaatatta agtttatcca
     1681 tttgtacccc cttggtttct atcctttctc ttgaacctgc catgttcttc aaatgactcc
     1741 tcctgttttt gggcacaaaa aaacagacct taacctgtct gttagaatat atgacacgac
     1801 cttccgatcg tgtccatagt acgaaataat tttgaaaggt taattctttt gttgcccgta
     1861 ttagattttt ttctccccat tcaatttaac ccttatcata atacgacaac tcctttcaaa
     1921 gtaacttttt acaagactag tataattcct atgtattccc atgtcaatag atcatttgca
     1981 ctttgccaga tatttcacat ttggccccta tcacagacct tcatgtatct ccagtacagg
     2041 aagtacaaac ataaatccag cagccttctt ttccttcatg ttaagaattt ccttgatgtt
     2101 tctaacagcc ttatcaagta cctccttgtc cttgattaca cttattaggg ttttgttgta
     2161 tggctttcct tcattgagga tttttgtgat gcttgaaaaa ataccttcat ccacattgtg
     2221 ctccatgagg accttg
//
LOCUS       DASRQF010000266         2570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_40162, whole genome shotgun sequence.
ACCESSION   DASRQF010000266 DASRQF010000000
VERSION     DASRQF010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40162"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VFD79_07080"
     CDS             <1..350
                     /locus_tag="VFD79_07080"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HZH93849.1"
                     /translation="SYKIKIMELAEKKKDIENQILEIIDKIIERSSILSHTTALTPFE
                     LEITSRCRMTGKSIGEINFWQNTGATIVGIRRGDQTILSPGPYATIEKGDILLIIGEE
                     EVFEAVKVFIDEG"
     gene            488..2122
                     /locus_tag="VFD79_07085"
     CDS             488..2122
                     /locus_tag="VFD79_07085"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZH93850.1"
                     /translation="MNKNELEKLFYDLVEIQSDTGTELEKDVEGFIYNWLSDLDYFKE
                     NKELFGKYQLSDDPLERAVVWGLRKGEGPDTVILLHHHDVVDSFDYGRIREFAYSPDK
                     LKDKISEADINQETREDLESGKWLFGRGTADMKAAGAIHMLLLKDYTELEDFKGNLLF
                     LSVPDEESLSQGAREGASLLNNLKDKHGLNYLMCIDGEPHERDEKGRAVFYEGSVGKT
                     MVVVYVRGRKTHLGHIFQGLNPSHVLSEIVTLTDMNPLFSDVVEGEVSPPPSWSFMRD
                     TKEQYDASIPESAGGYFSVLTLTQTPKEILENAKKLSTQAFDNVIKRINEHYSRFRIM
                     GHQPIENLPWSTNVMYFSEAFQQALDDSGEEFLKAYKEKVEVIKREIAEGKTNIPDST
                     LDLISLTIDFMIDKNPKVVIAFSPPYYPHIANLDFKNLPEKAKHLGLHLIQFAKEELG
                     QCYARKHYFMGISDLSYVALNDSESVVPYIEKNMPHWNRTYSIPFKDLEAISVPIVNI
                     GPWGKDYHKFTERVLREDLLENTPKLTRFAIDYLLS"
     gene            2176..>2570
                     /locus_tag="VFD79_07090"
     CDS             2176..>2570
                     /locus_tag="VFD79_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HZH93851.1"
                     /translation="MEAIINFVNWITGIVWGWPMLITLGLGGFILSARLGFFQIRYLP
                     YILKQTFGKMFGKVEKGEVSPFQAATAALASTVGASNIIGVPAAIMFGGPGAVFWMWV
                     MALMGMGSKFVEIVLGFKYREKNEEGEYVG"
BASE COUNT          821 a          450 c          618 g          681 t
ORIGIN      
        1 cctcttacaa aatcaagatc atggagcttg cggagaagaa gaaggatatc gagaaccaga
       61 ttcttgaaat aattgacaaa atcattgaaa ggagcagcat tttaagtcat accacagccc
      121 ttactccctt cgagctggaa attaccagta gatgtagaat gaccggtaaa tccatagggg
      181 aaattaactt ttggcaaaac actggagcta caattgttgg aatcaggagg ggcgaccaga
      241 caatcctttc tccaggacca tacgcaacca tagagaaggg tgacatcctc cttatcattg
      301 gggaggaaga ggtttttgaa gcggtaaagg tattcataga tgaaggttaa atgttgtaat
      361 aagtggctct cgataataat atatcgagag ttctttattt gaccgatgca gtaaagttat
      421 gtataataaa aaacaagtgt taatatttga ataattcaaa cgaattccaa tatttgggag
      481 tgatctgatg aataagaatg aactggaaaa actgttttat gatttagtgg agattcaaag
      541 tgataccggt acggagctgg aaaaggatgt agaaggtttt atctataatt ggttaagtga
      601 tttggactat tttaaggaga acaaggaatt gttcggaaaa tatcaattaa gtgatgaccc
      661 tcttgaaagg gcagttgttt ggggacttag gaaaggagaa ggacccgata ctgtaatatt
      721 gcttcatcat catgatgttg ttgactcatt tgactacgga agaatcaggg agtttgcata
      781 tagtccggac aagctgaaag ataagatatc cgaagcggac attaaccagg aaacaaggga
      841 agaccttgaa tcgggaaaat ggctatttgg aaggggaaca gctgacatga aggcggccgg
      901 agcaatccat atgctcctgc ttaaggacta cactgagctt gaagatttta aagggaatct
      961 tttattcttg tctgtacccg atgaggaatc actttcccag ggtgcaaggg aaggtgccag
     1021 ccttcttaac aatctaaagg ataagcatgg acttaactat ctgatgtgca ttgatgggga
     1081 accccatgaa agagatgaga agggaagggc agtattctat gaaggttccg taggcaagac
     1141 catggttgta gtatacgtaa gagggcgaaa gacccatctg ggacatattt tccagggtct
     1201 gaacccgtca catgtattat ctgaaatagt gaccttgaca gacatgaatc ccctgttttc
     1261 agatgttgtg gaaggagagg tgtctccacc accatcatgg agttttatga gagatacaaa
     1321 ggaacagtat gatgcttcaa ttccagaatc tgcaggtggt tatttttcag ttttgactct
     1381 gacccagacg cccaaggaga ttctagagaa tgctaaaaag ctgtctactc aagcttttga
     1441 caatgtaatc aaacggatta atgagcatta cagcaggttc aggataatgg gccaccagcc
     1501 aatagaaaat cttccctgga gtacaaatgt aatgtatttt tctgaagcct tccaacaggc
     1561 tctggatgat tcaggggaag aattcctcaa ggcctacaaa gagaaggttg aagtgataaa
     1621 gagggagatt gcagaaggaa agacaaacat cccagacagc accctggatc tcataagcct
     1681 gaccatagac tttatgattg ataagaatcc aaaggttgta atagcatttt cacctccata
     1741 ttatccccat attgcaaatc tggatttcaa gaatcttcca gaaaaagcca agcacctcgg
     1801 tcttcacctg attcaatttg ccaaggaaga gctgggacaa tgctatgcca ggaaacatta
     1861 cttcatggga atatcagacc ttagctatgt agctctcaat gacagtgaat cagtggtacc
     1921 atatatagaa aagaacatgc ctcattggaa caggacatac tccatcccgt tcaaggatct
     1981 tgaggccata tcggttccaa tagtaaacat tggaccttgg ggcaaggact accataagtt
     2041 cactgagagg gtgctaaggg aggacctgtt ggagaataca cccaagctga caagatttgc
     2101 aatagattat cttttatcat agtgatttta aatgcatagg gcatttagaa tataaaaatt
     2161 caaaggaggt attgtatgga agcaattatt aattttgtaa actggattac cggtattgtg
     2221 tggggatggc ccatgcttat cacactgggt ctgggaggat tcatcctgtc tgctagactg
     2281 ggatttttcc agattcgtta cttaccctac atcttgaagc aaactttcgg gaagatgttt
     2341 ggcaaggttg aaaaaggaga agtttcacca ttccaggcag caacagctgc acttgcttca
     2401 accgtaggag catcgaacat aattggtgta cctgcagcaa ttatgtttgg tggacctgga
     2461 gccgttttct ggatgtgggt tatggccctt atgggaatgg gatcaaaatt tgttgaaatc
     2521 gtacttggat tcaagtacag ggaaaagaac gaagagggcg agtacgtcgg
//
LOCUS       DASRQF010000267         2074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1781766, whole genome shotgun sequence.
ACCESSION   DASRQF010000267 DASRQF010000000
VERSION     DASRQF010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1781766"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1462)
                     /locus_tag="VFD79_07095"
     CDS             complement(149..1462)
                     /locus_tag="VFD79_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HZH93852.1"
                     /translation="MDLFTMSMEDRLKKDAPLADRMRPENIDEFVGQDHILGEGKFLN
                     RAIKADRINSMIFYGPPGTGKTTLAMIIANQTKMQFEKLSAVTAGVKDIREVVARAEE
                     SLKLRGKKTILFVDEIHRFNKSQQDALLPFVERGIIILIGATTENPYFEVNKALLSRA
                     MVLLLHPLERKHLDQLLDRALRDKDKGMGDHRVILTDEARDYLLSIAQGDGRILLNSI
                     EIGVLSTEPGEDGTRVVDLEGIKESIQVKTAKYDKGGDEHYDTISAFIKSMRGSDPDA
                     TLYYLAKMINAGEDPIFIARRIIIAASEDVGNADPQALQIATAALQAVHAIGMPEGRI
                     ILSQAALYVATAPKSNRAYIGIDKALSDIRNKEIGAVPLHLKDAHYGGAKALGHGKTY
                     LYPHDYPGGHVDQTYLPEGMEDRVYYEPTEHGYEKKIKDGMKKKR"
     gene            complement(1556..>2074)
                     /locus_tag="VFD79_07100"
     CDS             complement(1556..>2074)
                     /locus_tag="VFD79_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HZH93853.1"
                     /translation="RVEEKFNIELEIISGGNSSTVPLLDGRIPEGINHLRMGEAFLMG
                     TEAAYGNRIPGTDDSVFTLEAQVIEVKEKPSVPTEEIGRDAFGKVPTFVDRGVRKRIL
                     CAIGKQDMDLDTLRPTDPGILILGGSSDHLILDGSDSQKDYRVGDIISFKMHYVSMLR
                     IMTSEYVEKRII"
BASE COUNT          450 a          557 c          463 g          604 t
ORIGIN      
        1 ttgacttgca tgtctgagcg aagcgagttt gctagtcaat tttcgaaacg agtgtgatga
       61 atctctgaga ttgaatccga gagcggttca ctatactttc cgagaataaa attgagatct
      121 ctccacaagg tcgagatgac attgtgaatt atcttttctt cttcatccca tccttgatct
      181 tcttctcata accatgttca gtgggctcgt agtataccct gtcctccatt ccctctggca
      241 ggtaggtctg gtccacatgt cctcccggat agtcgtgggg atacaggtag gtcttcccat
      301 gacccaaggc cttggccccg ccgtaatggg catccttaag gtgaaggggt acagcaccaa
      361 tctccttgtt tcttatatcc gacagggcct tgtcaatgcc gatgtaagcc ctgttgctct
      421 ttggtgctgt ggccacgtag agggcagcct gagacagtat gatccttcct tccggcattc
      481 caatggcatg gactgcctgg agggctgcag tggcaatctg aagggcctgg gggtctgcgt
      541 tgcccacatc ctccgaagcc gctataataa tccttctggc aatgaatatg gggtcctccc
      601 cggcattgat catcttggca agatagtaaa gggtggcatc tgggtctgac cccctcatgc
      661 tcttaataaa ggcagatatg gtatcgtagt gctcatcccc acccttgtcg tacttggcag
      721 tcttgacctg gatggattcc tttattccct caaggtccac caccctggtc ccgtcctctc
      781 cgggctctgt tgacaggact ccaatctcaa ttgagttgag gaggatcctt ccgtctccct
      841 gggctatgga aaggaggtag tccctggctt catcagtcag tattaccctg tggtctccca
      901 tgcccttgtc cttgtccctc agggccctgt ctaatagctg gtccaggtgt tttctttcca
      961 aggggtgtag aaggaggacc atggcccttg aaaggagggc cttgttaacc tcaaaatatg
     1021 gattctccgt agtggcccca atcaggatta ttattcccct ttcaacaaag gggaggaggg
     1081 catcctgctg ggacttgttg aacctgtgta tctcatccac aaagaggatt gtctttttcc
     1141 ctctaagctt taggctctcc tcagcccttg caacaacctc cctaatgtcc ttgaccccgg
     1201 ctgtaactgc cgaaagcttc tcaaactgca tttttgtctg gtttgcaatt atcattgcaa
     1261 gtgtggtctt gcccgttcct ggtgggccgt agaatatcat ggagtttatc ctgtcggcct
     1321 ttattgccct gttgaggaac ttaccctctc caagtatatg gtcctgcccc acgaactcat
     1381 ctatgttttc cggtctcatc ctgtctgcaa ggggggcatc tttcttaagc ctatcctcca
     1441 ttgacatggt aaataaatcc attagatcac ctatttcagt ttattctttt acataccacc
     1501 attataacac aagttccagg tattccaaga aaatgaaaag gagccccaag ggcccctaga
     1561 ttattctctt ttcaacatat tcgcttgtca ttatcctaag catgctgaca tagtgcatct
     1621 taaaggatat gatgtcacca accctgtaat ccttctggga atctgacccg tcaagaatca
     1681 ggtggtcgga gcttccccca agtatcaaaa taccaggatc agttggtctt agggtatcca
     1741 ggtccatgtc ctgcttgcct attgcacaga ggatcctctt ccttaccccc ctgtccacaa
     1801 aggtcggaac ctttccaaag gcgtcccgtc ctatctcttc agttggcaca gatggcttct
     1861 ccttgacctc aatgacctgg gcctcaaggg tgaatacaga atcgtcagtt cctggaatcc
     1921 tgttgccata ggcagcctca gttcccatca ggaaggcttc tcccattctc aggtggttta
     1981 ttccctcagg gattctacca tcaagcaggg gtacagtact ggagtttcct ccagaaatga
     2041 tttcaagctc gatattgaac ttctcctcta ccct
//
LOCUS       DASRQF010000268         8776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_165815, whole genome shotgun sequence.
ACCESSION   DASRQF010000268 DASRQF010000000
VERSION     DASRQF010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 8776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8776
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165815"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..147
                     /locus_tag="VFD79_07105"
     CDS             <1..147
                     /locus_tag="VFD79_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004834590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HZH93854.1"
                     /translation="LKANKTYNEIEEETGASTATISRINRSLNYGADGYKVVFEKLGL
                     IEKE"
     gene            complement(194..1003)
                     /locus_tag="VFD79_07110"
     CDS             complement(194..1003)
                     /locus_tag="VFD79_07110"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HZH93855.1"
                     /translation="MLIALIGGLLALYLYAIKPNPPSKKNLEPFLGPYYAHRGLFDNA
                     SSSPENSLVAISKAVDKGYGVEFDVRLTRDGVPVVIHDNSLKRSLGLDGLVSEMTYKD
                     LSEFNLFGSNEKIPTLQEVLSVVSGRVPIIVELKQDHGGDTTICHIAAPILDNYKGDY
                     MVESFNPVLMIWYKKNRPAVIRGQLSTNHLKEGKKGLLLNLSLQYLLFNFLVKPDFIA
                     YNHIYGNNLSLFINRRLYGITTAAYTLKSQDELDKNRTRFDILIFDSFTPE"
     gene            complement(1015..2295)
                     /locus_tag="VFD79_07115"
     CDS             complement(1015..2295)
                     /locus_tag="VFD79_07115"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZH93856.1"
                     /translation="MKLNYKRTFFIGLAFMSISSFWQLYDNIIPLILQNTFGIGETFT
                     GAIMAMDNLLALFLLPIFGAFSDRVDTRFGKRTPFIVLGTLGAVTFMLLIPISNNLRS
                     FPLFFISLGAVLLAMSTYRSPAVALMPDLTPKPLRSKANSIINLMGAVGAIFALAVIS
                     LLVPDEPNPSYFSVFLIISLLMVLSVLVLVKTIKEKRLSEEMKEFNSTQDLDDDENTD
                     SDGEMAPDVKRSLYFILASIFLWFTAYNAVTTAFSRYAGRVWGLQGGSFANALLVATG
                     AAILSYIPIGIIASKVGRKRTIIAGIVLMSTSYFLGYLFLEYSPMINAVFAITGIGWA
                     AISVNSYPMVVEMSKSADVGKYTGLYYTFSMSAQIFTPILSGFFLENVGYRTLFPYSV
                     FFSLASLATMLMVRHGDSRPPKKGSTLDHFDVDD"
     gene            2455..2889
                     /locus_tag="VFD79_07120"
     CDS             2455..2889
                     /locus_tag="VFD79_07120"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZH93857.1"
                     /translation="MDDYRLKNQLIEALGKVYYMEAFSQLVEFLQGELYVLYYLAMRE
                     DIEASPSEISERLHMSRPRTTATLTALRKKGFVVTRANERDRRRVDVMITKKGKDHLE
                     RKKDAVNENLDLFISGIGKEEALHLIRIINKAAGLMETERGI"
     gene            2889..3593
                     /locus_tag="VFD79_07125"
     CDS             2889..3593
                     /locus_tag="VFD79_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZH93858.1"
                     /translation="MKDFVVLENVTKYYYMGNHKIEAAKDISFTIDKGEFCVIVGPSG
                     AGKTTVLNILGGMDSCDGGRIVLDGLVVSSLNERQLTDYRRYDVGFVFQFYNLVQNLT
                     ALENVELASQICKDPLDPATVLSQVGLSERMDNFPAQLSGGEQQRVSIARALAKNPKI
                     LLCDEPTGALDYETGKNILKLLQDSCREFGKTVILITHNQAISQMADRVIHLKNGQVS
                     EMSTNPAPLPIERIEW"
     gene            3587..7504
                     /locus_tag="VFD79_07130"
     CDS             3587..7504
                     /locus_tag="VFD79_07130"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZH93859.1"
                     /translation="MVVKNAYGKIVSREFIESFGRFAAIFGIVALGVGFLSGLLVTTP
                     DMHNSVDEYYDMNNMADIFIKGTMGITDDDLQTVAELEEVSEVMPAYVLDMLVDTSQN
                     GTLVTRIYGLPLIRGEANINRLELLEGRMPLKSNEVLVERSGTYLAEIPLGSTITISS
                     ENEDYEDIEDYFRVLTYEVVGVVGNTFHFSTEREVTNVGSGRLGAIIYSDQSSYALEN
                     WTDLYITAKDAISLDSFSNEYGVKIQRLVDKLDGISLERSEIRYGEVLREAEEELADG
                     WEEYKDGKAEADQELADAWQDILEGRADLVDGLKDLQDGEKELADARITLRDEVADAE
                     REIAEGEEDLADAYMELRDGEKELADALVELQDGEKEYYEGYLEFLDGKRKYEDGLEE
                     YLDGEKEYAKGLEKLQDGKRELARGERDLQRGRRELQAGEDEYAAGAKDLQEGKDQLL
                     AGVSQIAENFKLEGFTPQDLVGTASGQAVLSGAIGGAKDELEAKQVEIEGNIEGIKQQ
                     ISYYESQLETTTDSAIIIGLEEGLDEAKTNLAAAEAGLTHINAQLEELPDPNQLIGGW
                     NQILSGERELEYARRQLDDGWDEYYDGRAHLRDAQKELQEGEKELEDARKELDDAKAE
                     LEDAEKELRDGELELADGRRELDDGWKEYNDGLLDIQDGWKEYKDGLVEIEDAKVTLA
                     EEVADAEREIADGRRELADGWREYNDGVVDLQEGEAEYFEEKSKAEEELADAYADLMD
                     AEREIADLEMPEWYVLDRESNMSFVNFQLNADKVAAIAKFFPIFFYLVAALVSLTTMT
                     RMIEEERTQIGILKALGFRRTSIIGKYVAYCSLASILGSAAGQLVGFKLIPRVIWNAY
                     GVMFHLPPFVSEYNARIAFVASGIAVLSTVGVTVYAANEALGEKPATLMLPRAPKAGK
                     RILLERIGPLWRKMSFNMKSTARNIFRYKKHFYMTVVGISGCTALLVMGFGMRDSIRD
                     FAGSQFDHIFMYQINVELDEEELSKEPLDQLVENLDHVESYTGVYSDKGIVRHNNDLL
                     DVNLRVFHDPEGLSENILIRDRESREIILPGAEYVIITEKLSEVLGVSIGDTILYEDS
                     DGNRGQLKVTNITENYLGNYLYVDFAQYEAVFGPSPTNSALVRIGDLTGDEEDGFVER
                     LLLEDSVRNVELLSQTRRSFDNLVRSIDYIVLVIIVASGLLAFIVLYNLTNINVNERK
                     KELATLKVLGFHNEEVTSHIFREIAILTLIGIGVGLVLGTYMHEFIIKTIEDPDTMFG
                     RDIRWMSFVLSAVITLIFSFIVNLFMGRKIRNIEMVESMKAND"
     gene            8014..>8776
                     /locus_tag="VFD79_07135"
     CDS             8014..>8776
                     /locus_tag="VFD79_07135"
                     /inference="COORDINATES: protein motif:HMM:NF014750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HZH93860.1"
                     /translation="MNGYNGKFLFVDLSKKEITTESFDDQFVRDYIGGYGFGAKILYD
                     RMPAGADPLGEDNILGFITGMTNGTGAMYGGRFTMVHKSPVTGGWNDSNSGGFFGPEL
                     KKAGFDGLFIHGKAEKPVYIWVKDGEVEIRDASHLWGMDTKEVWDVLKEDHEEPAAKI
                     VAIGPSGERQSLLACPINDGHRAPGRGGGGAVMGSKNLKAVLVRGTGNVAVADPERLK
                     EVNKLVLKAMKENKGTKRFSDMGTGGGTPAAALSGD"
BASE COUNT         2596 a         1692 c         2318 g         2170 t
ORIGIN      
        1 ttgaaggcca acaagaccta caatgaaatt gaagaggaaa caggtgcttc aactgccaca
       61 atcagccgta tcaacaggtc acttaactat ggggctgatg gatacaaggt tgtatttgaa
      121 aagcttggtc taattgaaaa ggaataagaa caaaggattt ctcaggggag aaatcctttt
      181 cctatttctt gaactattcc ggagtgaagc tgtcaaatat caggatatca aaccgggtcc
      241 tgtttttatc aagctcatct tgggacttta aagtatatgc agccgttgtg attccataga
      301 gccttcgatt tatgaaaagt gacaggttgt ttccgtagat gtggttgtag gcaataaaat
      361 caggcttaac cagaaaattg aacagaaggt actggaggga caggttaaga agcagaccct
      421 tcttgccctc cttcagatga tttgtggaca gctgccccct gatgacggca ggtctatttt
      481 ttttgtacca aatcataagg acgggattga aggattccac catataatcc cccttgtagt
      541 tgtcaagaat cggtgctgca atatggcata tggtcgtgtc tcctccgtgg tcttgcttaa
      601 gctccactat gattggaacc cttccggaca ccactgaaag aacctcttgt aatgttggga
      661 ttttctcatt tgaaccaaaa agattaaact cacttaggtc tttgtaggtc atttcagata
      721 ccagcccatc aagtcccaaa cttctcttaa ggctattgtc atgaatcacc actggaaccc
      781 catcccttgt aagcctcaca tcaaactcaa ccccgtagcc cttatccact gccttggaaa
      841 tggcaacaag ggagttttct ggactactgg aggcattgtc aaataatccc ctgtgggcat
      901 agtagggtcc aaggaagggc tctagatttt tcttgctggg tgggtttggc tttattgcat
      961 aaaggtacaa ggccagtagt cctccaataa gtgcaattaa catgaaatcc tcctttagtc
     1021 gtcaacatca aagtgatcaa gtgtactccc cttctttggc ggcctagaat ctccgtgcct
     1081 gaccattagc atggtcgcaa gggatgccag ggagaagaag accgagtagg ggaacagggt
     1141 cctgtaacct acattctcaa ggaagaagcc agagagtatg ggggtgaata tttgggccga
     1201 catggaaaat gtgtagtaaa ggcctgtata ttttccaaca tccgcacttt tactcatttc
     1261 aacaaccatg gggtatgagt ttacacttat ggccgcccaa ccaatacccg ttattgcaaa
     1321 cacggcatta atcattggcg agtactcaag gaagagatag cccagaaaat atgatgtgct
     1381 catcaggaca atccctgcaa ttatagtcct ctttctgccc accttgctgg caattattcc
     1441 gattggaata tagctaagta ttgcagctcc agttgccacc agaagggcat ttgcaaagct
     1501 tccaccctgt agtccccaca cccgtccggc ataccttgaa aaggctgttg taacggcatt
     1561 gtatgctgta aaccaaagga aaatggaggc caggatgaaa tacaggctcc ttttaacatc
     1621 aggtgccatt tctccatcac tgtcggtgtt ctcatcatca tcaagatcct gtgtagagtt
     1681 gaactccttc atttcttccg aaagtctctt ttccttaatg gtcttgacaa ggaccaggac
     1741 agataggacc atgagaagtg atattataag aaatacgctg aaatagctgg ggtttggctc
     1801 atctggcacc aggagcgaaa tcactgcaag ggcaaatatt gctccaactg cccccatcag
     1861 gttgataatg gagttggcct tgctcctaag cggctttgga gtaaggtcgg gcataagagc
     1921 aactgcaggg gacctatagg tgctcattgc cagaagaact gcccccaggg atatgaaaaa
     1981 caggggaaag ctcctgagat tatttgagat tggtatcagt agcataaatg ttacggctcc
     2041 tagggttcct aaaacaatga agggagttct ttttccaaac ctggtatcaa ccctgtccga
     2101 gaaggcgccg aatatgggca ggaggaaaag ggccaaaagg ttgtccatgg ccatgatggc
     2161 tcctgtaaat gtctctccga taccaaaggt gttctgaaga atcaggggga tgatattgtc
     2221 gtacaactgc caaaacgatg aaatggacat aaaagcaagg cctatgaaaa atgtgcgttt
     2281 ataatttagc ttcaagactc cacctcctaa gaatgtataa ttcaattata cagcatagag
     2341 gcctggtgtg aatttttttt gttaagaata aataatatct cacataagaa agggtctgcc
     2401 gtggtataat taatagtatt aacaattaat agcattaact aaggagggat aaggatggat
     2461 gattatagac tgaagaacca gctgattgaa gcccttggaa aggtctacta catggaggca
     2521 ttttcacaac ttgtggagtt tctccagggc gagctttatg tcctgtatta ccttgcaatg
     2581 agggaggata tagaagccag cccgtcagag atcagtgaaa ggctacatat gtcaaggcca
     2641 aggaccactg caacccttac tgctttaagg aagaagggct ttgtggtcac aagggcaaat
     2701 gaaagggaca ggcggagggt cgatgtgatg ataacaaaaa aaggcaagga ccaccttgaa
     2761 aggaagaagg atgcggtaaa tgaaaacctt gaccttttca taagtgggat cggtaaagag
     2821 gaagcgctcc atctgatcag gataatcaat aaggcagcag gacttatgga gactgagagg
     2881 ggtatctgat gaaggatttt gtagttttgg aaaatgtaac taaatactat tatatgggaa
     2941 accacaagat tgaagctgcc aaagacataa gcttcaccat tgacaagggt gaattctgcg
     3001 taattgtagg gccttcaggt gccgggaaga ccacagttct aaatatttta ggtggcatgg
     3061 actcctgtga tggaggaaga atagtccttg atggactggt tgtgagcagc cttaacgagc
     3121 gccagctaac ggactacaga agatatgacg taggctttgt attccagttc tacaacctgg
     3181 tccagaatct tacggcactg gagaatgtgg agcttgcctc ccagatttgc aaggacccac
     3241 ttgaccctgc aactgtcctc tcccaggttg gtttaagtga gcgtatggac aatttccccg
     3301 cccagctttc aggtggagag cagcaaaggg tttcaattgc cagggcactt gcaaagaacc
     3361 caaagatact actgtgcgac gagcccactg gagcccttga ctatgagaca gggaaaaata
     3421 ttctgaagct actccaggat tcctgcaggg agtttggaaa gacggtcata cttataacac
     3481 ataatcaggc tatatcccag atggcggaca gggttataca cctgaagaac ggacaggtat
     3541 cggagatgtc aacaaaccca gcccccttgc caattgaaag gattgaatgg tagtgaaaaa
     3601 cgcatatgga aagattgtat ccagagaatt cattgaaagc tttggacgat ttgcagccat
     3661 atttgggatt gtggcactgg gcgttggatt tctttcagga cttctggtaa caacaccgga
     3721 tatgcataat tcagttgatg aatactacga catgaacaat atggctgaca tcttcataaa
     3781 gggtaccatg ggtatcacag atgatgacct tcaaacagtt gcagaactgg aggaggtcag
     3841 cgaagtcatg cccgcatatg tgctggatat gcttgtagat acctcccaaa acgggacact
     3901 tgtcacaagg atatatggtt tgccattaat aaggggagaa gcgaacataa acaggctgga
     3961 actgcttgag ggaaggatgc ccctaaaatc caatgaggtc cttgttgaaa gaagcggtac
     4021 ctatcttgcc gaaattccct tgggatctac aataaccatt tcttctgaga atgaggacta
     4081 tgaggatata gaggactact ttagagtcct tacctatgag gtggttggag ttgtgggcaa
     4141 caccttccat ttctccactg aaagggaagt gaccaatgtt ggaagtggaa gactgggagc
     4201 aatcatctac tcagaccagt ccagctatgc attggaaaac tggacagacc tctatattac
     4261 agcaaaggac gccatttcac tggactcctt ctccaatgag tatggagtta aaatccagcg
     4321 tttggttgac aagcttgatg gaatatccct ggaaagatca gagataagat atggggaagt
     4381 cctgagggag gcagaggagg agcttgccga tggctgggag gaatacaagg atggaaaggc
     4441 agaggcagac caggagcttg ccgatgcctg gcaggacatc cttgagggaa gggcggacct
     4501 tgtagatgga ttaaaagacc ttcaggatgg ggaaaaggaa cttgcagatg ccagaataac
     4561 ccttagggat gaggtggcag atgcagagag ggagatagca gaaggagaag aggaccttgc
     4621 tgatgcctat atggagctta gggatggcga gaaagaattg gctgatgccc ttgttgaact
     4681 ccaggacggg gaaaaggaat actatgaagg atatctggag tttttggatg ggaagaggaa
     4741 atatgaggat ggcctagagg aataccttga tggagaaaag gagtatgcca agggccttga
     4801 aaagctccag gatggaaaaa gggagcttgc aaggggtgaa agagacctcc agcggggaag
     4861 aagagagctc caggctggag aggatgagta tgcagcagga gcgaaagacc tccaggaagg
     4921 caaggaccag ctccttgcag gagtgtccca gattgcagaa aatttcaagc tggagggctt
     4981 cactccccag gacctggttg gtacagcctc gggacaggct gttcttagtg gtgcaatcgg
     5041 tggggccaag gatgagctgg aggcaaaaca agttgagatt gaagggaata ttgagggtat
     5101 taagcagcaa attagttatt atgagagcca gcttgagact acaacggatt cggcaataat
     5161 aattggcctt gaagaagggc ttgatgaagc gaaaacaaat cttgccgcag ctgaagcagg
     5221 attgacccat ataaacgccc aactggaaga gctcccagac ccaaaccagc ttatcggtgg
     5281 ctggaaccag atcctgtcag gagaaaggga attggaatat gccagaagac agctggatga
     5341 tggctgggat gagtactatg atggaagagc ccacctaagg gatgcccaga aggaactcca
     5401 agaaggggag aaggagcttg aggatgccag aaaagagctt gatgatgcaa aggctgagct
     5461 tgaggatgca gaaaaggaat taagagacgg agagctggaa cttgccgatg gaagaagaga
     5521 acttgacgat ggctggaagg aatacaatga tggattgtta gacatccagg atggctggaa
     5581 ggagtacaag gacggcctgg ttgaaattga ggatgcaaag gttacacttg cagaagaggt
     5641 ggcagatgct gaaagggaga tagccgatgg aagaagagaa ctggctgatg gctggaggga
     5701 atacaatgat ggagtggttg acctccagga aggggaagct gaatattttg aggagaagtc
     5761 caaggcagag gaagagcttg cagatgccta tgcagaccta atggatgctg aaagggaaat
     5821 agcggacctg gagatgcctg agtggtacgt tttggaccgg gaatcaaata tgagctttgt
     5881 aaacttccaa ttgaacgcgg ataaggttgc tgccattgcc aagttctttc caatattctt
     5941 ctatcttgtg gcagccctgg tttcactgac cacaatgacc agaatgatag aggaggaaag
     6001 gacccaaata gggatcctta aggcgttggg tttcaggagg acatcaataa tcggaaaata
     6061 tgtagcctac tgcagtcttg caagcatact gggaagtgca gcaggccagt tggttggatt
     6121 taagctgata ccaagggtta tctggaatgc atacggagtg atgttccacc ttcctccctt
     6181 tgtttcagaa tataatgcca gaattgcatt tgtggcatcg ggaattgcag tcttgagtac
     6241 agttggagtg actgtatatg cggcaaatga agccctgggg gagaaaccgg ctaccctcat
     6301 gctgccaagg gcaccaaagg caggcaagag gatactcctg gagagaattg gacccctatg
     6361 gcgtaaaatg agtttcaaca tgaagtccac tgccaggaac atattcaggt acaagaagca
     6421 tttctatatg acggttgtgg gaatatctgg atgtacggcc ctgcttgtga tgggatttgg
     6481 catgagggat tcaatacgtg attttgcggg cagtcagttt gaccatatat ttatgtacca
     6541 gataaatgtg gaacttgatg aggaagaatt gtccaaagaa ccacttgacc agctggtgga
     6601 gaacctggac catgtggaat catacacagg agtgtactct gataagggga tagtaagaca
     6661 taacaatgat ctcctggatg ttaacttaag ggtgtttcat gacccagaag ggctttcaga
     6721 gaatatcctc ataagggatc gggagtccag ggagattata ctgcctgggg cagagtatgt
     6781 gataataaca gaaaagctgt cggaggttct tggggtatcc atcggtgata caatcctata
     6841 tgaggactct gatggaaacc gtggccagct taaggtaacg aatatcactg aaaactatct
     6901 aggcaactac ttgtatgtgg atttcgccca gtatgaggcc gtgtttggac catcacccac
     6961 aaatagcgcc cttgtaagga taggagattt gacgggtgat gaagaggatg gttttgtgga
     7021 gcggctgctt ttggaggact cggttcgtaa tgttgaattg ctgagccaga ccagaaggag
     7081 tttcgacaac cttgttagga gcatagacta cattgtctta gtcattattg ttgcctctgg
     7141 actacttgca tttattgtcc tttacaatct taccaatata aatgtcaatg aaaggaaaaa
     7201 ggagcttgca accctcaagg tgctcggttt tcacaatgaa gaggtaacct cacacatctt
     7261 cagggagatt gccattctca ccttgattgg aattggtgtg ggtttggttc ttggaaccta
     7321 tatgcatgag ttcatcatca agaccattga ggacccggat acaatgtttg gtagggatat
     7381 cagatggatg agttttgtgc tttcagccgt tatcaccctg atattctcct ttatagttaa
     7441 cctgtttatg ggtcgtaaga taaggaacat tgaaatggtt gagtcaatga aagcaaacga
     7501 ttaattttaa gcctggggag acctgggctt ttcttttttt gattttcctt tgcattgata
     7561 ctatccttgc taatccaagg tgacataaga atttgtcatc ctgaagcttg ctgaaggatc
     7621 tttgacccca caattcgact caactgtcat ttcggcagtc atataataat tagcccttag
     7681 gtatttgcac gcagttcttg attcaagtcc gattaaaatc atcagctcgt tttctccatt
     7741 gattcctatg ccgcttcgct tggaggaatc aaggcacttc gaaatcctct ctggatttta
     7801 aagtcgttct ttcatatgaa ctgctttgca aagtaccatt cgggctttga aaactgttat
     7861 gtcatcctga agcttgctga aggatctgag tccctacagc ttgactaatt gattgtgaaa
     7921 tgtgaattgt gatttgtgaa ttgtttatga acttactgaa aattgcaaca aacggacaaa
     7981 ttttgtataa tatcactatg gagagtgatg aaaatgaatg gatacaatgg aaaattttta
     8041 tttgtggacc tgagcaaaaa ggaaattacc actgaaagct ttgatgacca gtttgtcagg
     8101 gactacatag gaggatatgg ctttggagca aagatactct atgacaggat gccggcaggt
     8161 gcagatcccc ttggagagga caacatcctg ggatttatca ctggaatgac caacgggact
     8221 ggagccatgt atggtggaag gttcaccatg gtccacaaat ccccagtgac aggaggctgg
     8281 aatgattcaa actcaggagg cttctttgga cctgagctta aaaaggccgg atttgacgga
     8341 cttttcatcc atgggaaggc agaaaagccc gtatacatct gggtcaagga tggagaggtt
     8401 gaaatcaggg atgcaagcca cctttgggga atggatacaa aggaagtttg ggatgtcctt
     8461 aaggaagacc acgaggagcc tgctgcaaag attgttgcaa tcggaccctc aggagagagg
     8521 cagtccctcc ttgcatgtcc aattaacgac ggacacaggg caccaggtag aggtggcgga
     8581 ggagctgtta tgggctcgaa gaaccttaag gcagttcttg taaggggaac cggcaatgta
     8641 gcagttgcag atccggaaag gcttaaggag gtaaacaagc tggtccttaa ggcaatgaag
     8701 gaaaacaagg gaacaaagag gttcagcgac atgggaacgg gaggaggaac ccctgctgct
     8761 gccctgagcg gagata
//
LOCUS       DASRQF010000269        13486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_77057, whole genome shotgun sequence.
ACCESSION   DASRQF010000269 DASRQF010000000
VERSION     DASRQF010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 13486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13486
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77057"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..585
                     /locus_tag="VFD79_07140"
     CDS             169..585
                     /locus_tag="VFD79_07140"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZH93861.1"
                     /translation="MQRMKCSWKEGMTFSASDGLGHELLMDLGKEAGGNNSGFRPMPL
                     LLAGLGGCMGVDVKIILDRMKVSFTGLELEIEADMDETTLPRIYREIRVIFRFEGENL
                     EIDKIERAIDLAQEKYCNVSAILGKTCSLVYKAEIL"
     gene            597..1835
                     /locus_tag="VFD79_07145"
     CDS             597..1835
                     /locus_tag="VFD79_07145"
                     /inference="COORDINATES: protein motif:HMM:NF006771.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HZH93862.1"
                     /translation="MKTKVERIKKDIEELAKFNSSTEGGLTRFSLTREDRMAREYLKD
                     QLCKLDVKIYEDNAGTLFARREGTDPQAPAIMIGSHFDSVKNGGNFDGPAGVVMALEI
                     FRALEENNIKTRYPLEFVAMIEEEGGRFGSGVFGSRAMAGLVDYDQLERNRDRDGISM
                     AQALRDFGFEPEKVAEAKRNKEDVKAFIELHIEQGPILEKEGKDLGIVDFIVGINEFR
                     VQVDGRPDHAGTTPMDMRADALGVASKVVAEIDSYPVPENNGTVATVGVLEIKPGAAN
                     IVPAQVVFTVDIRSKDEGSIANVKKKIMARLEALTEGTDVTYTVTEMLDVKPVKMNEE
                     MVRLFKESAEEKGFTYRNMLSGAGHDAMVMAAVTEVGLVFVPSRDGRSHCKEEWTDYD
                     QLQKGIELIYDTILKLGEAK"
     gene            1835..2998
                     /locus_tag="VFD79_07150"
     CDS             1835..2998
                     /locus_tag="VFD79_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004822704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HZH93863.1"
                     /translation="MNVKELVEKYLDYSVAMRREFHMNPEPSMKEYKTQKRVMEELEK
                     MGIPAEKCGGTGVVGLIKGKGEGKTIALRADMDALEVTELNDVEYKSKVEGVMHACGH
                     DAHTAGLLTAAKVLNDMKDEFNGTVKLLFQPGEEIAQGALAMIKDGTMEGVDGIMGIH
                     IWNDVETGKISVEAGPRMASTGMFKVYVEGRGGHGSMPHQTVDAAVVASAIVMNLQSI
                     ASRETNPLDPAVVSVGMIKSGTRWNVISGDAYMEGTTRCFSMELNDAFEGIIKRVVES
                     TAEAYRAKARVEYTQMTIPTINEEKMSKLAAETVKKLHGEENLVKFEKTTGGEDFAFF
                     SEYAPSLFAFVGSKNEDKHEYHPHHHPKFNIDEDALGRAAELYAQFALDFLNS"
     gene            complement(3134..4987)
                     /locus_tag="VFD79_07155"
     CDS             complement(3134..4987)
                     /locus_tag="VFD79_07155"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZH93864.1"
                     /translation="MTDTGQRIQELQNILEATKLLNASRDLDFILRELMNTTLRLISR
                     ADIGIIFLYDKSDGLLKSKFSFGFDDMKVTLKPGESITGITFNRKKTLHLTSPEEVHR
                     AMSSMSGDTREMLHRRIIRPMDRLLSAISCPLIHDGECIGVLVLDNYIGNDPFTEDDV
                     YLAELISVQATIAIKNAENYSREIAAQNSLKEYSQLVEKEKNRYQYSTFLHNKFTEMI
                     LNRSSSSDILNEVSQLLQTDLFIVDLFYSIVSYAGNHHHKKDDLEDIKPSIMEYLSSE
                     GETIHYNQDHGLWIMLYPIFVSEDVFGWIGILLEDDNLSELDKIVIEKCANVVALDIL
                     KEYDMANLEQSIRGDYFDNLLKGTPINLDNRFQQMYQIDFSHNHRMILVNLIYHKKDN
                     PAYKYFRYFYQAVNQQAIQKFKRSLTIQKRNLIIIIVDDNDAFNRELAEDFHNSILQI
                     VAKLESRANLQSSCVSIVSEKITGYEEFRSIYERTYKLFEIIPNRSLSGCHFYEDYLV
                     KKILLKNDKEDLIDFARKILAPVMEYSHSSRIELFETLKTYIFNGGNWTKTKNDLHIH
                     GNTLTYRLNRLKELLDADIDDYQTRLRIQLALEITVIYNEILEDIKQAARS"
     gene            complement(5009..6232)
                     /gene="tyrS"
                     /locus_tag="VFD79_07160"
     CDS             complement(5009..6232)
                     /gene="tyrS"
                     /locus_tag="VFD79_07160"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HZH93865.1"
                     /translation="MRNVFDVLKERGYIDQATDEEALRDLLEKESVAFYIGFDATADS
                     LTLGHFLQIMVMMHMQKAGHRPIALLGGGTTMIGDPSGKSDMRRVLTREMIDYNAERF
                     MEQFSRFIDFSEDKAFMPNNADWLMDLNFVEFMREIGVHFSVNRMLTFDCYKNRLEKG
                     LTFFEFGYMLMQSYDFLHLYRTYDCKLQLGGSDQWSNILGGYELVRKLEQDKVYGMTF
                     KLLTTAAGTKMGKTEKGTIWMDRTKTTPYELFQYLRNVDDRDVEKFLALLTFLPMEEV
                     RRLGALEGAEINKAKEVLAYEVTSMIHSKEDADGALEAARALFADGMDESNIPSTQLD
                     PERFKDGVGILDLMREVGMIKTNSEGRRLVQQGGVTIDGEKVDAVDRMVILDDFKDGK
                     ILIKKGKKTYHQVQI"
     gene            complement(6517..6891)
                     /locus_tag="VFD79_07165"
     CDS             complement(6517..6891)
                     /locus_tag="VFD79_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II SORL domain-containing protein"
                     /protein_id="HZH93866.1"
                     /translation="MSIGNLYQSGDWKGEKHVPVIHAPEMVKKGEEVEVKVLVGEEIA
                     HPNTLEHYIAWIKVYFKPEEGKFPVEIGTYTFAAHGESELFTAPYLTTKFKVSTPGTL
                     YATSYCNIHGLWENSMEIKVED"
     gene            7014..8144
                     /locus_tag="VFD79_07170"
     CDS             7014..8144
                     /locus_tag="VFD79_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HZH93867.1"
                     /translation="MDRIELIATTTFGLEAVTKRELIDLGVDDINVENGKVSFWGSLR
                     DIPRTNLWLRTAERVLVKMGEFKALTFDELFEKTKALPWEEWIPEDGNFIVEGKSVKS
                     KLFSISDSQRIVEKAVVERLKTKYPVEWFNKTGPRYRIEVSLLKDIATLTIDTSGEGL
                     HKRGYRDRAGDAPIKETLAAAMVMLSYWNKDRVLFDPFCGSGTILIEAAMIGKNIAPG
                     LDRKFDAENWDIIPKDYWAEEKVEAMRKIDHDTTLHLLGSDTDKRSILRARDNAANVG
                     LEDDIAFFMKDMRDVDLQEEYGVVITNPPYGERMGDREEVEQLYRDFGKKFSDHPTWS
                     IYAITSNENFEALYGKKADRKRKLYNGRIKVDYYQFYGPRPE"
     gene            8160..8684
                     /locus_tag="VFD79_07175"
     CDS             8160..8684
                     /locus_tag="VFD79_07175"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HZH93868.1"
                     /translation="MGIYTKTGDKGTTSLFDNKRVTKDDIRVESYGTVDELGTFLGLA
                     KNYVEDELRERIYTIQNKLFTVATNLATEDGSKVAHRMKEEDIKYLEDTIDEYMGRLN
                     DPTGFIIPGSNISSAYLHVARTVCRRAERRIISLTGVAEVDPLVVKYVNRLSDAIYAM
                     ARYSEEEQRPVKYE"
     gene            8879..10009
                     /locus_tag="VFD79_07180"
     CDS             8879..10009
                     /locus_tag="VFD79_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZH93869.1"
                     /translation="MIEEKKAVDISEDLTYIPYGLLNYRVLEGMADWVRVINRNGKVI
                     FANAAMKEALGNDMVGSDCYKVHCKKEKCGFCITNRSIETGETVQKEEIIQGQYYSVK
                     SSPVKNQHGEIAGAVEVFRNVTRERKLELELIERNKKISRDLHFARRIQERVLPKRGM
                     VETLRIDYVYQASEMLSGDMFDIFYIDEANIGIYICDVAGHGIAASMMTMFIKQAMRS
                     IKDEVLSPGLALEELHKRFSTLQLEADKYFTIFYGVYNKVTHRFTYSNAGHNCYPIKF
                     NSEEIEPLKIKGYPITLLFEKVEYKEKEIKLDPGDKILLYTDGITEARDHNDKQFGIE
                     GVLETVQKNPDNILKEVEDKFIIHSWGEQDDDYALVLVEVVD"
     gene            10084..11388
                     /locus_tag="VFD79_07185"
     CDS             10084..11388
                     /locus_tag="VFD79_07185"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HZH93870.1"
                     /translation="MTVGFDYSKSFIKKHEVDFMKPFLKEAHNLLHDRDGPGNEFIGW
                     LDWTEKYDRQEYEKIKTVAAGIRQKADVFIVVGIGGSYLGARAAFEALTHTFHNLLPR
                     EERQAPQIYFAGNNMSPKYMTDLMETLEDKEVYVNVISKSGTTTEPAIAFRILKSHLE
                     DKYGKEEARNRIFATTDGSKGALKTLATNEGYETFVIPDDIGGRYSVLTPVGLLPLSV
                     AGIDIDQLMEGAHEAAIQYGNPNLDENRCYQYAVTRQILHKRGKDIEILVNYEPGLQY
                     LGEWWKQLYGESEGKDGKGVFPASVNFTADLHSMGQLIQDGKRNIFETSIVIENAERD
                     LEISHDEENLDGLNYLQGKTIGHVNRKAFEGTLLAHLEGNVPNLIISLPLLDEFHFGK
                     LIYFFEKACAISGYLQGVNPFDQPGVEAYKKNMFKLLGKEGY"
     gene            11448..12227
                     /locus_tag="VFD79_07190"
     CDS             11448..12227
                     /locus_tag="VFD79_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZH93871.1"
                     /translation="MEFKHLVIEKENKVGVLRINRPEAMNSLNTKVLEELLMAVEQVK
                     NDQDIFVLVITGEGKAFVAGADISEMSTMDPVQARSFAEAGLKVFREIESMEKPVVAA
                     VNGFALGGGCELAMACDIRIASTKAKFGQPEVGLGVTPGFGGTVRLSRLVGPAKAKEL
                     IYSGNIIKADEALGIGLVNSVSEPEELMGAAMEMANKISSNAQLAVRYSKQSINRSFE
                     TDVETAMEIERNLFGLCFSTEDQKEGMKAFGEKRKPDFKVK"
     gene            12241..13089
                     /locus_tag="VFD79_07195"
     CDS             12241..13089
                     /locus_tag="VFD79_07195"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HZH93872.1"
                     /translation="MKKVSVLGRGTMALGIVQIFAQKDIEVTVWVRSIDPEKPRGSVA
                     PIEKALGKLVSKEKIRQEQMDKTLANIKITTELKDVSDSDLVIEAISEDMKVKKDTFG
                     KLDEICKPEAILATNTSSLSITEIANSTARPDKVIGMHFFNPVPLMKLVEVIKGLATS
                     DETKDKIVDLSKSLGKTPVEVEEAPGFVVNRILIPMINEAVGILADGVAKAEDIDEAM
                     KLGANHPIGPLALGDLVGLDVCLAIMDVLYEEYQDSKYRAHPLLRKMVRGNLLGRKTG
                     KGFYAY"
     gene            13221..>13486
                     /locus_tag="VFD79_07200"
     CDS             13221..>13486
                     /locus_tag="VFD79_07200"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93873.1"
                     /translation="MRLYNSPRGVVEFINFKEMKMEDLVLGLINPMPVIALLAIIGVS
                     LFTLSKGADLLIDEAVAISGYLKVPKAIIGATIVSLGTTLPELSV"
BASE COUNT         4086 a         2618 c         3304 g         3478 t
ORIGIN      
        1 agagaattcc ttatggatga aatgcttcca aagatgcttg ttggaggatt tacaacagta
       61 acaatcgtat ccgtaataat tgcgggaatt ttcgtaaatc tgatttagca aaaagcgcct
      121 tttcaggcgc tttttataca atcggaatca aaggaaaggt ggagtcatat gcaaaggatg
      181 aaatgctcct ggaaagaggg aatgaccttt tctgccagtg acggtcttgg tcatgaattg
      241 ttgatggacc ttggcaagga ggcaggtggg aataattcgg gattcaggcc aatgcccctt
      301 ctgcttgcgg gacttggcgg ctgcatgggt gttgatgtta agataatatt ggataggatg
      361 aaggtatcat ttacaggatt ggaacttgaa attgaggcag acatggatga aacaaccctt
      421 ccaaggatat atagggaaat aagggtcatt ttcagatttg aaggtgagaa tctggaaatt
      481 gacaagattg aaagggcaat cgaccttgcc caggagaagt attgcaatgt aagtgcaata
      541 cttggaaaaa cctgtagtct tgtatataag gcagaaatac tctaggaggc tgatttatga
      601 aaacaaaggt tgagagaata aaaaaggata ttgaggaact ggctaaattc aacagcagca
      661 ctgagggtgg cttgacaaga ttttccctaa ccagggagga cagaatggca agggaatacc
      721 ttaaggacca gctttgcaag ctggatgtaa aaatttatga ggacaacgct ggaaccctct
      781 ttgcaagaag ggaagggaca gacccccagg ctccagcaat catgattggt tcacattttg
      841 actcagtgaa aaatggaggt aacttcgacg gccctgcagg tgttgtaatg gctcttgaaa
      901 tattcagggc actggaggag aataatataa aaaccagata tcctcttgaa tttgtagcaa
      961 tgatagagga ggaaggcgga aggtttgggt ctggagtttt cggaagccgt gcaatggcag
     1021 gacttgtcga ttatgaccag ctggagagga acagggacag ggatgggata tccatggccc
     1081 aagcccttag ggactttgga tttgagcctg agaaagttgc agaagccaag cgtaacaagg
     1141 aagatgtaaa ggcatttatt gagctgcata tcgagcaggg acctatactg gaaaaggaag
     1201 gtaaggacct gggtattgta gatttcatag tgggaataaa tgagttcagg gtccaggttg
     1261 acgggagacc ggaccatgct ggaaccacac ccatggatat gagggcagat gccctgggtg
     1321 tggcctcaaa ggttgtggct gaaattgatt cttacccggt tcctgaaaac aatggaaccg
     1381 ttgcaaccgt cggagttctt gagatcaagc caggtgcggc caacatagtt cctgcccagg
     1441 ttgtttttac agttgacata agatccaagg atgaggggag tatagcaaat gtaaagaaga
     1501 aaatcatggc taggctggaa gcccttacag agggaactga tgtaacctat accgtgacag
     1561 agatgctgga tgtgaagcca gtgaagatga atgaggaaat ggtccgtctc tttaaggagt
     1621 ctgctgagga gaagggcttc acatacagaa acatgctcag tggagcagga cacgatgcaa
     1681 tggtaatggc tgctgtaaca gaggttgggt tggtctttgt tccaagcagg gatggacgta
     1741 gtcattgcaa ggaggaatgg acagactacg atcagttaca aaaaggaata gaactgattt
     1801 acgataccat actaaaacta ggggaggcaa aataatgaat gtaaaagagc ttgtagaaaa
     1861 gtacctggac tactccgtag ccatgagaag agagttccat atgaatcctg agccaagcat
     1921 gaaggagtac aagactcaga agagagtcat ggaagagctt gaaaagatgg gtatacctgc
     1981 ggaaaagtgt ggaggtaccg gcgtagttgg actgataaag ggaaagggag aaggaaaaac
     2041 catagcccta agggcggaca tggatgcact tgaggtaact gaactgaacg atgttgaata
     2101 caagtcaaag gttgaaggtg taatgcatgc ctgcggacac gacgcacata cggcaggact
     2161 ccttacagct gccaaggtcc taaacgacat gaaggatgag ttcaatggaa cagttaagct
     2221 cctattccag ccaggtgagg agattgccca gggagccctt gccatgatca aggatggaac
     2281 gatggagggt gtggatggaa taatgggaat ccatatctgg aacgatgttg agacgggaaa
     2341 gatttcagtg gaggctggcc ctagaatggc ttcgacagga atgttcaagg tttatgttga
     2401 gggcagggga ggacatggtt caatgcctca tcagactgta gatgctgctg ttgttgcatc
     2461 ggcaatagtc atgaaccttc agtctatcgc cagcagggag accaatccac ttgatcctgc
     2521 cgtagtaagc gttggaatga tcaagagcgg gactcgatgg aatgttattt caggggatgc
     2581 atacatggaa ggtacaacca ggtgtttctc catggaactg aatgatgcct ttgagggaat
     2641 aatcaaaagg gttgtggaga gcacagcaga agcctacagg gcaaaggcca gggttgagta
     2701 cacgcagatg acaattccaa ccataaatga agagaaaatg tctaaattgg ctgcagaaac
     2761 agtcaagaag ctacacggtg aagaaaacct ggtgaagttt gaaaagacaa ctggtggaga
     2821 ggattttgct ttcttctcag aatacgcacc ttccctattc gcctttgttg gatctaaaaa
     2881 tgaagacaag cacgaatatc acccccacca ccatccaaag ttcaacatcg acgaggatgc
     2941 actgggaagg gcagctgagc tttacgcaca gtttgcactg gactttctta acagctaata
     3001 ggacagaggt aggacatagg acagagggac aggaacactg tcttaggaca gagggacagg
     3061 aacactgtct tagacagata ggacagaggg acaggaacac tgtcttatta acctaaaaac
     3121 aagctgccag gtctcaagac ctggcagctt gtttaatatc ctcaagaatt tcgttgtata
     3181 ttacagttat ctccaaagcc agctgaattc ttagccttgt ctggtaatca tctatatcag
     3241 catctagaag ttccttaagc ctgttcaacc tataggttag ggtgttccca tgaatatgca
     3301 gatcgttttt ggtcttggtc cagttacctc cattgaaaat gtaagtcttc agagtctcaa
     3361 agagctcaat tctggaagag tgcgaatact ccataaccgg agcaagtatc tttctggcaa
     3421 aatcaataag atcctccttg tcatttttca gtagaatctt cttgaccaga taatcctcat
     3481 agaagtgaca accggaaaga gacctgttgg gtatgatttc aaacagcttg taggttcttt
     3541 cgtagatgct cctgaactcc tcatagcctg ttattttctc actgacaatc gaaacgcagc
     3601 tcgattgtag atttgccctt gattcaagct tggctacaat ctgcaaaatg ctgttgtgaa
     3661 aatcctccgc aagctcccgg ttgaaggcgt cattatcatc aacaattatt attataagat
     3721 ttcgtttctg aatcgtaaga ctcctcttga atttctgaat agcctgttgg ttaactgcct
     3781 ggtagaagta cctgaagtac ttataggctg ggttatcctt tttatggtag atcaggttta
     3841 ccagtatcat cctgtggttg tggctgaagt ctatttggta catttgctga aagcggttgt
     3901 ctaaattgat gggggtcccc tttaggagat tgtcaaaata atcccccctg atggactgct
     3961 ccaggtttgc catgtcatac tccttgagta tatcaagggc cacaacattc gcgcatttct
     4021 caataacaat cttatcaagt tcagagagat tatcatcttc aaggaggatg ccaatccatc
     4081 cgaacacatc ttccgataca aaaatggggt aaagcataat ccatagacca tggtcctggt
     4141 tatagtggat cgtttcaccc tctgaagaca agtactccat tatagatggc ttgatatcct
     4201 ccaaatcatc cttcttgtgg tgatggtttc cagcatagga gacaattgaa tagaataagt
     4261 cgactatgaa gaggtctgtt tgcaaaagtt gagaaacctc attgagtatg tcacttgatg
     4321 aacttctgtt caatatcatt tctgtaaact tgttatgcag aaaggtcgag tattgatacc
     4381 tgttcttttc cttttccaca agctggctgt attctttaag ggagttctgt gctgctatct
     4441 cccttgaata gttttctgca tttttgattg cgattgtagc ctggactgaa atcagctcag
     4501 ccagatacac atcatcctct gtaaatgggt cattgccaat gtaattatcc agtaccagga
     4561 ccccaataca ttccccatca tgaatcaagg gacagctgat ggcactaagg agcctgtcca
     4621 tgggacggat gatccttctg tgcagcatct ccctggtgtc acccgacatg gatgacatag
     4681 ccctatgaac ctcctctggg gaggtaagat gaagtgtctt tttccggttg aatgttattc
     4741 cagtaattga ttcccctggc tttaacgtaa ccttcatatc gtcaaagcca aaggaaaatt
     4801 ttgacttgag caggccgtca gatttatcat aaaggaatat tatgccaatg tccgctctac
     4861 ttataagtct taaggttgta ttcataagct cccttagaat aaagtccagg tccctggacg
     4921 cattgagcag cttggttgct tcaaggatat tctgaagctc ctgtattctt tgtcctgtat
     4981 ccgtcatggt aaatctacct ttcaaatttt agatttgcac ctggtggtag gtctttttgc
     5041 cctttttaat caggatctta ccgtccttga aatcatcaag aatgaccatc ctgtcaacgg
     5101 catccacctt ttcgccatcg attgtgacac cgccctgttg aaccagacgt cttccctcgc
     5161 tattggtttt tatcattcca acctctctca taaggtctag gattcccact ccatccttga
     5221 atctttcagg gtccaattgg gtggaaggaa tattgctttc atccatcccg tctgcaaaca
     5281 gggccctggc agcctccaat gctccgtcag catcttcctt gctatgaatc atacttgtaa
     5341 cctcatatgc aaggacttcc tttgccttgt tgatctctgc cccttcaagg gcacccagtc
     5401 ttcttacctc ttccatggga aggaaggtga gcagtgcaag gaacttctcc acatccctgt
     5461 cgtctacatt tctgagatat tggaaaagct cgtaaggagt tgttttggtc ctatccatcc
     5521 agatggttcc cttttcggtt ttgcccatct ttgtccctgc ggccgtagtc agaagcttga
     5581 aggtcattcc gtagactttg tcctgctcaa gctttctaac aagctcataa ccgcctagga
     5641 tatttgacca ctggtcactt cctccaagct gaagcttgca gtcataggtc ctgtaaaggt
     5701 gcaggaagtc atagctttgc atcagcatat atccgaactc aaagaaggta agtccctttt
     5761 caagtcggtt tttgtagcag tcaaaggtca gcatcctgtt tacggaaaag tgtactccga
     5821 tttccctcat gaattcaaca aaattaaggt ccatcagcca gtcggcattg tttggcataa
     5881 aggccttatc ctcgctgaaa tcgatgaatc ttgaaaactg ctccatgaat ctttcagcat
     5941 tgtaatcgat catctccctg gtcaggaccc ttctcatatc ggattttcca gaagggtctc
     6001 caatcatggt tgtcccgcct cccaaaagcg caattggcct atgacctgcc ttttgcatgt
     6061 gcatcatgac catgatctga agaaaatgtc caagagtaag gctgtctgct gttgcatcga
     6121 acccgatgta aaaagcaact gattcctttt caagaaggtc tcttaaagcc tcttcatccg
     6181 ttgcctgatc aatgtatccc ctttccttca atacatcaaa aacatttctc attataattc
     6241 ctcctcacaa aaaaataaag ggccttctca tctaaaggac gagaaaaccc gtggtaccac
     6301 cttaattcac aaaaagcctc atatcacgta tggtgtgaaa tcccctgcaa actccaggct
     6361 gtatttggat aatgcgctat ctgtcggcct cccaccatgg ctgactctct gtgaagatcc
     6421 atcacaatat ctactctacc cttcatggtc cctatgaatt taataacata ttattatatg
     6481 aatggtccct tgtcaagata agaaaaagag gactgtttag tcctctacct tgatttccat
     6541 gctgttttcc caaagtccgt ggatgttgca gtagcttgtt gcataaaggg tacctggtgt
     6601 tgaaacctta aacttagtag taaggtatgg agctgtaaac aactctgatt ccccatgtgc
     6661 agcaaatgtg taggttccga tttccactgg gaatttacct tcctcaggct tgaaatagac
     6721 ctttatccaa gctatgtagt gctcaagagt gttgggatgt gcaatttcct cacccacaag
     6781 aaccttaacc tcaacctctt ctcctttttt aaccatttct ggcgcgtgga taacgggaac
     6841 gtgtttttca cccttccagt ctccgctttg atataaatta cctattgaca tgctcattcc
     6901 tccttcttta atttacctat tgtgatgtat accctaaaag acctaaaaaa cacaaattta
     6961 ttattaaaga tggcatttga ttggtataat agtacaagag gggagagaat agaatggata
     7021 gaatagaact gattgccaca accacatttg gattggaggc agtaacaaaa agagaattga
     7081 tagatcttgg agtggatgac ataaacgtag agaatgggaa agtatccttc tggggttctt
     7141 taagggatat acccagaaca aacctctggc tgagaacagc tgaaagggtc ctggttaaaa
     7201 tgggggagtt caaagcttta acctttgatg aactctttga gaagaccaag gcattgccct
     7261 gggaggaatg gattccagag gatgggaact ttatagtaga gggaaagagt gtcaagtcca
     7321 agcttttcag catatccgac agtcagagaa tcgttgagaa ggctgttgtg gaaagactca
     7381 agaccaagta tcctgttgaa tggttcaaca aaaccggacc caggtacaga atagaagtgt
     7441 ccctactaaa ggatatcgct acccttacca tcgacacatc aggcgaaggc ctccacaaga
     7501 gaggctacag ggacagggcc ggtgatgcac cgatcaagga gacccttgcg gcagctatgg
     7561 taatgctaag ctactggaat aaggacaggg tgctttttga tcccttctgc ggttcgggaa
     7621 caatcctcat agaggcggcc atgataggca agaatattgc tcctgggctt gacagaaagt
     7681 ttgatgcaga gaactgggac ataataccaa aggactactg ggcagaggaa aaggttgagg
     7741 ccatgaggaa aattgaccat gacaccacac tccacctcct gggatcagac acggataaaa
     7801 ggtcgatctt aagggccaga gacaacgctg caaatgtcgg ccttgaggat gacatagctt
     7861 tctttatgaa agatatgagg gatgtggacc tgcaggagga atacggcgtg gtcataacca
     7921 atccacccta tggtgaaaga atgggagacc gggaagaggt ggagcagttg tacagggatt
     7981 tcgggaaaaa gttcagtgac cacccaacct ggtcgattta tgccataacc tcaaatgaaa
     8041 actttgaagc cctttatggc aaaaaggccg acaggaagag aaagttatac aatggaagaa
     8101 tcaaggtaga ctattaccag ttttatggtc caagacctga ataagaaagg atgatttaaa
     8161 tgggaatcta tactaagaca ggcgacaagg ggacgacatc tttatttgac aacaagagag
     8221 taaccaagga tgacataagg gttgaaagct acggaaccgt tgatgagctg gggacatttc
     8281 tgggacttgc aaaaaactat gttgaggatg aactgaggga aaggatctat acaatccaga
     8341 acaagctttt cacagtggca accaatctgg caactgagga cggatccaag gtggcccata
     8401 gaatgaagga agaggacata aagtatctcg aggatacaat tgacgagtat atgggtagac
     8461 ttaacgatcc aacaggcttt atcatacctg gttccaatat ttcttcagcc tacctgcatg
     8521 tagccaggac tgtttgtaga agggctgaaa gaaggataat ctcactaaca ggagttgcag
     8581 aggtggatcc tttggtggtg aaatatgtta acagattatc agatgcaata tacgcaatgg
     8641 ccagatattc ggaagaggag caaagaccag ttaaatacga ataatccaca aaagtgtaaa
     8701 agggaatgat ttctccggca agggccttga aaatgccctt tgtttaccat gggaacatga
     8761 attttcttgt ggaaataaac tctgatttag gatacaatga taatagtcac cacatatttg
     8821 acattattgt atgtgacata atagttagaa tattaacgac ctcaaagagg tgattattat
     8881 gattgaagaa aaaaaggctg tggacatatc tgaagattta acatatattc cctatggact
     8941 gctgaattat agggttcttg agggaatggc tgactgggtg agggtaatta ataggaatgg
     9001 caaggtaatc tttgcaaatg ctgcaatgaa ggaagctctc gggaatgaca tggtgggaag
     9061 tgactgctac aaggtccatt gcaaaaaaga gaaatgtggg ttttgcataa ccaataggag
     9121 cattgaaacc ggggaaacag tccagaaaga ggaaataatt cagggtcagt actattctgt
     9181 aaaaagctcg ccagtaaaaa accagcatgg agagattgct ggtgctgttg aagtgttcag
     9241 aaatgtcacc agggaaagaa agctcgagct tgagcttatt gaaaggaata aaaagatcag
     9301 cagggacctg cattttgcca gaaggataca ggaaagagtt cttcccaaga ggggaatggt
     9361 ggaaaccctg cgtattgact atgtctacca ggcatctgag atgctaagcg gagacatgtt
     9421 tgatatattc tacattgacg aggcgaacat aggaatatat atttgcgatg tggcaggaca
     9481 cggtattgct gcttcaatga tgaccatgtt cataaaacag gcaatgagga gtataaagga
     9541 cgaagtattg agtccgggcc tggcactgga ggagctccac aagagattca gcactctcca
     9601 gctggaagca gacaagtatt ttaccatatt ctatggagta tacaacaagg tgacccacag
     9661 attcacctat tccaacgctg ggcacaactg ctacccgatc aaattcaact cagaggagat
     9721 tgaacccttg aaaatcaagg gttacccaat aacattgctc tttgaaaagg ttgaatacaa
     9781 ggaaaaggaa atcaagctgg atccaggaga caagatattg ctgtatactg acggaataac
     9841 tgaagccaga gaccataatg acaagcaatt tggcatagag ggagttttgg agactgtcca
     9901 gaaaaaccct gacaatattt tgaaggaagt tgaagataaa ttcataatcc acagctgggg
     9961 agagcaggat gatgattatg cacttgtgct tgtggaagta gtagattaac ctagaattca
    10021 tattctaggt tttgttttaa ttgcaaaggg gaagtggaag gacaatattt gaggaggggt
    10081 aatatgacgg ttggttttga ttattcaaag agctttatca aaaagcatga ggtagacttt
    10141 atgaagccgt tcctgaagga agctcacaat ctcctgcacg acagagatgg acctggaaat
    10201 gagttcatag gctggttgga ctggacagag aaatacgaca gacaggaata cgaaaagata
    10261 aagactgttg cggcaggaat aagacagaag gcagatgttt tcatagtggt aggcatagga
    10321 ggctcctacc tgggtgctcg tgcagcattt gaagccctga cccatacctt ccacaacctt
    10381 ctaccaaggg aggagagaca agctcctcag atttactttg cagggaacaa tatgtctcct
    10441 aagtacatga cagacctgat ggagaccttg gaggacaagg aggtctatgt aaatgtaatt
    10501 tccaagtccg gcacaaccac tgagcctgca atagcattca ggatacttaa gtcccatctg
    10561 gaggacaagt atggaaagga agaggcaaga aacaggattt ttgccacaac tgatggcagc
    10621 aagggagcct tgaagacctt ggccacaaat gaaggctatg agacttttgt gattcctgat
    10681 gatatagggg gcaggtactc tgttcttact ccagttggtc tgcttccatt gtctgtggct
    10741 ggaatagaca tagaccagct tatggagggg gctcatgagg ctgccatcca atacgggaac
    10801 ccaaacctgg atgagaatag atgctaccag tatgctgtaa ccaggcaaat cctccacaaa
    10861 aggggaaagg atattgaaat ccttgtaaat tacgaacccg gactccaata ccttggagaa
    10921 tggtggaagc agctttacgg tgaatccgaa ggcaaggatg gaaagggagt attcccggca
    10981 agtgtgaact tcactgctga ccttcactcc atgggtcaac ttatacagga cggcaagagg
    11041 aacatatttg agaccagtat agtcattgaa aatgctgaga gggatcttga aatcagccat
    11101 gacgaggaaa acctggacgg cttgaactat ctccaaggca agaccattgg tcatgtaaac
    11161 agaaaggcct ttgaaggaac cctgttggca catcttgagg ggaatgttcc aaacctaata
    11221 atatcactgc ccctgttgga tgaattccac tttggcaagc tgatatattt cttcgaaaag
    11281 gcatgtgcaa tatcaggcta ccttcaaggt gtgaatccat ttgatcagcc tggtgtggaa
    11341 gcctacaaga aaaacatgtt caagctcctt ggcaaggaag gttattaatc ctcttgatta
    11401 aatatgatat ggctgttatg ataattatat aacaaggagg gattctgttg gagtttaaac
    11461 atttggttat tgaaaaggaa aacaaggttg gagttctccg gataaacagg cctgaagcca
    11521 tgaattccct caacacaaag gtgttggagg agctgttgat ggctgttgaa caggtcaaga
    11581 atgaccagga tatttttgtt cttgtgatta ccggagaagg aaaagctttt gtcgcagggg
    11641 ctgatatttc cgaaatgagc accatggatc cggtccaagc aagatccttt gcagaagcgg
    11701 gactaaaagt attcagggaa attgagtcca tggaaaagcc ggtggtagca gcggttaatg
    11761 gctttgctct gggaggtggc tgtgagcttg caatggcttg tgatatcagg attgcatcca
    11821 caaaagccaa gtttggtcaa cctgaggttg gtcttggagt tactcctgga tttggtggaa
    11881 ccgtacggtt gtcacgtctc gtcggtccgg caaaggcaaa ggagctcatc tactctggta
    11941 atatcataaa ggctgacgaa gccttgggaa ttggcctggt aaattcagtg tcagagcctg
    12001 aggaattgat gggtgcagca atggaaatgg caaataaaat atcttcaaat gcccagcttg
    12061 cagttagata ttcaaagcag tcaataaaca gaagctttga aacagacgtg gaaacagcca
    12121 tggaaattga aagaaacctc tttggccttt gcttttcaac agaggaccag aaggaaggca
    12181 tgaaagcatt tggagagaag agaaaacctg atttcaaggt aaaataaacg gaggtgctgt
    12241 atgaaaaaag taagcgtatt aggcagggga acaatggcat tgggtatcgt tcagatattt
    12301 gcccagaagg acattgaggt aactgtatgg gttaggtcaa tcgaccctga gaaaccaagg
    12361 ggaagtgttg ccccaataga aaaggccttg ggcaagctgg taagcaagga aaagataaga
    12421 caggagcaga tggacaaaac acttgcaaac atcaagataa caactgaact gaaggatgtt
    12481 tctgacagtg atcttgttat agaggcaatt tcagaggata tgaaggtaaa gaaggataca
    12541 ttcggaaagc ttgatgaaat ttgcaagccc gaagccatac ttgccacaaa cacctcttca
    12601 ctttctataa ctgaaatagc aaattcaaca gcaagacctg acaaggtaat aggaatgcac
    12661 ttctttaatc cagttccact aatgaagctt gttgaggtaa taaagggtct tgcaacttca
    12721 gatgaaacca aggataagat tgtagacctg tcaaaatccc ttggcaagac acctgttgag
    12781 gttgaggaag cacctgggtt tgtggtgaac aggatcctta ttcctatgat aaacgaagcg
    12841 gttggaatat tggcagacgg agtggcaaag gctgaggata ttgacgaggc aatgaagctt
    12901 ggcgccaatc acccgattgg tccattggct cttggagacc tggttggact tgatgtatgc
    12961 ttagccataa tggacgtcct ttatgaagaa taccaggatt caaaatacag ggctcatcct
    13021 cttctgagaa aaatggttag aggaaatctt ctgggaagaa aaacaggcaa aggcttctat
    13081 gcatattagt accactacat tgacaaaatg gatgtagtta tgatatattc agtctgttag
    13141 agggagtaac tgccctgatt gggaacttaa gtcaccaata acggaccagt ccggtcctgg
    13201 cttaagtggt ttaaaatcgt gtgagactct acaattcccc aaggggagtt gtagagttta
    13261 ttaattttaa ggagatgaaa atggaagatc tggttcttgg tttaataaat ccaatgcccg
    13321 taattgcgtt acttgcaata ataggtgtaa gtctattcac attgagtaag ggagcggacc
    13381 tcttaataga tgaagctgta gccatttcgg ggtacttgaa ggttccaaag gcaataatcg
    13441 gtgccacaat tgtctccctt ggaaccacat tgcctgagct ttccgt
//
LOCUS       DASRQF010000270         4150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_683934, whole genome shotgun sequence.
ACCESSION   DASRQF010000270 DASRQF010000000
VERSION     DASRQF010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683934"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VFD79_07205"
     CDS             <1..493
                     /locus_tag="VFD79_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HZH93874.1"
                     /translation="CVDIGANHTSVSIVEDGVIKVARVVERGMDFVIEEIKRKLPHLE
                     DKQVLEYMDKIDDISQKKTGLDDEVILTESLRDSLYGMLDMIEMIFRYYKTREVSNEI
                     DLVLLQGGLSKINGIDRLFHDFFEMEVSRLRSMEKVKTNCDLSQYANAIGGLIRLKEV
                     KKK"
     gene            490..1050
                     /locus_tag="VFD79_07210"
     CDS             490..1050
                     /locus_tag="VFD79_07210"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HZH93875.1"
                     /translation="MRDLNFFDPFIEKKQFAFNKMLLLYGLLFIVIIGLGVMVAMNQL
                     QINALQKEVNSLRQVAEDPKTVARVAEIQAFEDETAQFRQEVERIRTLDKNIQARDVI
                     GERLLDDINSKLPEGVFLGNLSVSGREIRIAGFAEDRYSIAEFSKGLESIPEASKSFV
                     SNISAVESYYRFNLDLTLEEVLVDVN"
     gene            1040..2503
                     /locus_tag="VFD79_07215"
     CDS             1040..2503
                     /locus_tag="VFD79_07215"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HZH93876.1"
                     /translation="MSTKLKSIFSSIKTRPLTKSEKILLTLLTIVGIAFLGNQYVLVP
                     QDEKIQALQVDKYELETKIMEMNATLRREDDIKKEWEMLHRERNQILSYYFPTLDQAQ
                     IIYLLNDLLPEDQVEIADLNFSRPSSEKLSEMDVYNMGISVPFSGEYSGIEEMVRSIE
                     TSPRRMMVDSLSLDRSADNQLSGSMSLKVYSLEGLAEMEEEDVIFVDIADNTSQGTLF
                     SSFDGFVGSTGSGGTGGSGGTGGTDGGTAGGGTSGGGTGVDGGTDVKGDVLHSFEWRN
                     YDFVPSHPLVRGQAEPTTIALDGRYAMRLEYNIVGTEEENRAMVDITSQDIELKFPPT
                     DLSIHTYSFTYAPGTVGVRIINQDGDEAFIPVNEGISWLGWGRTRINLPGSVAEYPIK
                     ITHIYYEMDKGRDDFGVLVFDKLEAIYPHHVETAYEGTANEPDTIFYEVKPGESVSTI
                     SRDVYGTMEYKNEIMRNNGIQAGDVLPVGKILVLVKR"
     gene            2517..>4150
                     /locus_tag="VFD79_07220"
     CDS             2517..>4150
                     /locus_tag="VFD79_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HZH93877.1"
                     /translation="MNRIRLTLVMTIVLTLLLSGGVLAAQADYDFGYDEGKRFFGIDS
                     SFDDGDDAFDEYLDYIEDDDPELYDEIDSLGRSSYNDFQEGFIEGYNDAMEAGISVAD
                     ASSLGESLGRTAALTDYYDGNDSDWRDALPSDRNISRMFDLDRMSTSYEKSFISDFKS
                     GFQKGYEDEFQQALLSPPMESMSQGLDDGEAAGEAMGASFAEKDYLLGSSMNYERDLP
                     RDSEIIERYRLRLGTNEYRDAFVEGFKKQYQITYNETFRSLHQDRSMAALESTQIPTS
                     GGEIIAADGIGLLVEPGTFYMPVYVTINQQNTGYYRFGSYIRASEIYSIKLDNPAATL
                     DDNKKITIAFPYYGEEYKAGIYKLIDERWQYIPSKVEGDAITAQVLPSTMSNREGTYA
                     VFMDENIRILTDVRDHWAKPEIETMVRRSIITGYPGYTHLDGTFKPSQSITRAEFLIL
                     LSRIENWTLPNYIVNATHFKDHAQFAQYDRIISYALSRGYVIGYTDGTFRPNNPISYY
                     EVQLIMGRVQGSYGFNWNSLATQMMYEKAFRSPSFD"
BASE COUNT         1286 a          793 c         1071 g         1000 t
ORIGIN      
        1 ctgtgttgat attggagcca accacacaag tgtatcaatt gttgaggatg gtgttataaa
       61 ggttgccagg gttgttgaga gagggatgga ttttgtaatc gaagagataa aaagaaagct
      121 gccacacctt gaggataaac aggtactcga atacatggat aaaattgatg atatctcaca
      181 gaaaaagact ggacttgatg acgaggttat ccttacagaa tccctaaggg acagcctgta
      241 tggaatgctt gatatgattg aaatgatatt caggtactac aagacaaggg aagtgagcaa
      301 tgaaattgac ctggtgctgc tccagggagg actttccaag ataaatggca tagacagact
      361 cttccacgac tttttcgaga tggaggtatc gaggctcagg tccatggaaa aggtaaagac
      421 aaactgtgac cttagccaat atgccaatgc aattggaggt ctcataaggc tgaaagaggt
      481 gaagaagaaa tgagagattt aaactttttt gatcccttca tagaaaagaa acaattcgcc
      541 tttaataaga tgctgctttt atatggacta ttatttattg taattatcgg actgggagtc
      601 atggtcgcca tgaaccagtt gcagatcaat gccctccaga aggaggtcaa cagccttaga
      661 caggttgcag aagaccctaa gacagttgca agggttgcag aaattcaggc ctttgaggac
      721 gagactgctc agttcagaca ggaagtggaa aggatcagga cccttgacaa gaacatccag
      781 gctagggatg taatagggga gagactcctt gatgatataa attcaaagct tcctgaggga
      841 gtatttcttg ggaacctgag tgtaagtgga agagaaataa ggattgctgg ctttgcagag
      901 gacaggtact caattgccga gttttccaag ggtcttgaaa gtatccctga agcatcaaag
      961 tcatttgtat caaatatttc cgcagtggaa agctactacc gattcaatct ggatcttaca
     1021 ctggaggagg tgctggtaga tgtcaactaa gttaaaatca atctttagca gcatcaaaac
     1081 cagacccctt acaaaaagtg agaaaatcct tcttacactt ttgaccattg taggcatagc
     1141 gttccttgga aaccagtatg tactggtacc ccaggatgag aagatacagg ccctgcaggt
     1201 ggacaagtac gagttggaaa caaaaattat ggaaatgaat gccactctca ggagagagga
     1261 tgacatcaag aaggaatggg agatgctaca cagggagaga aatcagattc tttcatacta
     1321 ttttccaacc cttgaccagg cacagataat ttatcttcta aacgacctgc ttcctgaaga
     1381 ccaggttgaa attgctgacc ttaacttttc aaggccatca tcggagaagc tctctgagat
     1441 ggatgtatac aatatgggta tttcagtacc cttcagcggt gaatattcag ggatagagga
     1501 aatggtaagg tccatagaaa ccagtccaag gaggatgatg gtggatagcc tatcactgga
     1561 caggagtgct gataaccagc tttcaggatc aatgagccta aaggtatatt ccctggaagg
     1621 acttgctgag atggaggaag aagatgtaat ctttgttgac attgcagaca atacaagtca
     1681 gggaaccctc ttctcaagct ttgatggatt tgttggatca acaggaagtg gaggcacagg
     1741 tggtagcggt ggaacaggcg gaaccgatgg cggtactgct ggaggaggaa catccggtgg
     1801 aggaaccggt gttgacggcg gaactgacgt gaagggtgat gtccttcaca gctttgaatg
     1861 gagaaactac gactttgttc caagtcatcc tttggtaagg ggacaggcag agccaactac
     1921 catagccctt gatggaagat atgcaatgag gcttgaatac aatatagttg gaaccgagga
     1981 agaaaacagg gcaatggtgg atataacaag ccaggatatt gagcttaagt tcccaccaac
     2041 agaccttagc atccatacct attcctttac ctacgctcct ggcactgttg gtgttaggat
     2101 aataaaccag gatggggatg aagcattcat tccagtcaat gaagggattt catggcttgg
     2161 atggggaagg accaggatca accttcctgg aagcgttgct gagtacccaa taaagatcac
     2221 ccatatatac tatgaaatgg acaagggaag ggacgatttt ggagtgcttg tatttgacaa
     2281 gcttgaggca atctatcccc accatgtgga aactgcctat gaggggactg caaatgaacc
     2341 tgacaccata ttctacgagg tcaagcctgg agaatccgtc tcaaccattt ccagggatgt
     2401 ttatggaacc atggaataca agaatgagat tatgagaaac aacggtatac aagctggaga
     2461 tgtactccca gttggcaaga tactggtact ggtgaagaga tagggggaga gatatcatga
     2521 acaggataag acttacatta gtgatgacta tagttttgac tctccttctt tcagggggag
     2581 tattggcagc acaggctgac tatgattttg gatatgatga gggtaaacgg ttctttggaa
     2641 tagactcgtc ctttgatgat ggagacgatg catttgacga gtaccttgac tatattgagg
     2701 atgacgatcc ggaactgtac gatgagattg atagccttgg acgtagctcc tacaatgatt
     2761 ttcaggaagg ctttattgaa ggctacaatg atgccatgga agctggcata agtgttgctg
     2821 atgcaagcag tctaggtgag agtcttggaa ggactgccgc cttaaccgac tattatgatg
     2881 gcaatgattc ggattggcga gatgcccttc ccagcgacag gaacatcagc aggatgtttg
     2941 atttggacag gatgagcacc agctatgaaa agagctttat ctctgacttt aaatctggat
     3001 tccagaaggg ctacgaggac gagttccagc aggcactcct aagtccccca atggaaagca
     3061 tgagccaggg gcttgatgat ggagaggctg caggtgaagc catgggggca tccttcgcgg
     3121 agaaggacta cctgcttgga agctctatga actatgaaag agacctgcca agggattcag
     3181 agatcattga gagatacagg ctgagacttg gtacaaatga atacagggat gcctttgttg
     3241 agggcttcaa gaagcaatac cagataacct acaatgagac cttcaggtct cttcaccagg
     3301 acaggtcaat ggcagcattg gagtcaaccc agataccgac ttcaggtgga gaaatcattg
     3361 cagcagatgg aatcggactt ctggtggagc caggaacctt ctacatgcct gtgtatgtaa
     3421 ctatcaacca gcagaataca ggctattaca ggtttgggtc ctatatcagg gcatctgaga
     3481 tttactcaat aaagctggat aatccagctg ctaccctaga tgataacaag aagataacaa
     3541 ttgcctttcc atactatgga gaagagtaca aggctggtat ctacaagctg attgatgaga
     3601 ggtggcagta catcccgtca aaggttgaag gagatgccat aacagcccag gtccttccaa
     3661 gcacaatgtc aaacagggaa ggcacctatg cggtatttat ggatgaaaac ataaggattc
     3721 tcaccgatgt aagggaccac tgggcaaagc ctgaaatcga gacaatggta agaagaagca
     3781 taataacagg ctatccaggc tatacacacc ttgatggaac cttcaagccg tcacagagca
     3841 taacaagggc agagttcctg atccttttga gcaggataga aaactggaca ctgccaaact
     3901 acattgtgaa tgcaacccat ttcaaggacc atgcacagtt cgcacagtac gacaggatca
     3961 ttagctatgc cctttcaagg ggctatgtaa taggctacac tgatggaacc ttcaggccta
     4021 acaatccaat ttcatattac gaggtacagc ttattatggg aagagtgcag ggaagctacg
     4081 gcttcaactg gaattcattg gcaacacaga tgatgtatga gaaggccttt aggtcaccga
     4141 gctttgacaa
//
LOCUS       DASRQF010000271         6418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_288578, whole genome shotgun sequence.
ACCESSION   DASRQF010000271 DASRQF010000000
VERSION     DASRQF010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6418
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288578"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VFD79_07225"
     CDS             <1..620
                     /locus_tag="VFD79_07225"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HZH93878.1"
                     /translation="KVRPDKAMGYMAAESASTEERSQGCVGCGTGASVGKILGPESSM
                     KSGLGSATVRAGDLVVSALIAVNSFGDVFHKGVQIAGVYDYTTDQLISTERIMKESTD
                     KIQFANTNTTIGVVATNALLTKAEGNKVAQTAHNGLARSINPVHTAVDGDTIFVMGTS
                     KVQADLNLVAMLAAEAVEKAVINAVMTAKPYGNMKTAGEISEKVL"
     gene            748..1497
                     /locus_tag="VFD79_07230"
     CDS             748..1497
                     /locus_tag="VFD79_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HZH93879.1"
                     /translation="MKKGSRFDIRKLTIIGVLGAISVVLGMTPLGFIPVGPTRATIMH
                     IPVIIGAIVEGPIVGALVGLIFGLFSIYQAITAPTPVSFVFLNPIVSVLPRVLIGITA
                     YYTYKGLNKLGTKGTIGMLYLIWAAIIAYLARGIYLSVADYTSIWPLLMNTGLLILTA
                     GMVYLTATKFKDKALDTVIAAVVGTMTNTVGVLTMIYIFFGERFVQALGQDVGLARKV
                     IVGIGITNGIPESIIAIIIVTSVVSAVKRRR"
     gene            1509..2285
                     /locus_tag="VFD79_07235"
     CDS             1509..2285
                     /locus_tag="VFD79_07235"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HZH93880.1"
                     /translation="MLLVIDVGNTNIVFGAYEGDKLVYDWRISTDKEKTSDEYGLLFD
                     QMFKYHGIVPSSVDDIIISSVVPTLMHTLPAMSNRYFGKDPIEIGPGVKTGMNIKYDN
                     PKEVGADRIVNAVAAYEKYGGPVIIVDFGTAITFCFVNKEGEYLGGVITPGIKIAAEA
                     LFLRTAKLPKVEIIKPEKAIGKTTIGSIQSGLVYGFTGMVDSLIERMVKEMNHTMDEV
                     KVVATGGFSTMIAGESKYIQYNDKYLTLEGLRIIYERNSK"
     gene            2324..3319
                     /gene="dusB"
                     /locus_tag="VFD79_07240"
     CDS             2324..3319
                     /gene="dusB"
                     /locus_tag="VFD79_07240"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HZH93881.1"
                     /translation="MKIGSFNIENKLMLAPMAGVTDLAFRIICKEMGVGLTVTEMVSA
                     KGMYYNDKKTDSLTQIDPRERPVALQIFGSDPEIMGEVVRNKLNYRDDIDIIDINMGC
                     PAPKIVKNGDGSALMRNPKLAEDVIRSVAKEANKPVTVKIRKGWDQSTVNGVEIAKIA
                     EASGAAAITIHARTRDMFYEGKADWDYIGRIKDAVGIPVIGNGDIFTPQDAIDMMDRT
                     GCDGVAIGRGAMGNPWIFRQTNQLINGDEPVKPSYTEVLETSIRHMNMACEIKGDRLG
                     VREMRKQLAWYLKGMRNSNEVKNQINTMEDRKEIEFLLRTYIKHLTDGDNSHSGL"
     gene            3515..3991
                     /gene="greA"
                     /locus_tag="VFD79_07245"
     CDS             3515..3991
                     /gene="greA"
                     /locus_tag="VFD79_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HZH93882.1"
                     /translation="MAEKDVFLTREGLAKLENELEDAKIVKRKEIAERIRTALDFGDI
                     SENSEYDQAKNEQAKLEERIAKIEGILRNAKLIEDEEISTDIVGIGSRVVVLDVEYDE
                     EMEYTIVGSAEANPYEGKISNESPVGKALLGSKAGDVVDVQVPDGVIKYQIQKIAR"
     gene            4009..5913
                     /gene="lysS"
                     /locus_tag="VFD79_07250"
     CDS             4009..5913
                     /gene="lysS"
                     /locus_tag="VFD79_07250"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005957911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HZH93883.1"
                     /translation="MQTGEQSLNEMLQIRREKLAKLIEKGKNPFLIEKYDYSHHSSNI
                     KDNFEELEGTEVSVAGRIMSRRGHGKVSFIDIQDSEGRIQVFAKQDLIGEEEYEDLSF
                     LDLGDIIGAKGTIFKTKTGEVSVRATEIVLLTKSLQILPEKFHGLKDQDLRYRQRYVD
                     LIVNPEVKDTFILRTKIIKGVREYLDDRGFLEVETPILSTIAGGANARPFETHHNTLD
                     VDMYLRIANELYLKRLIVGGFEKVYEMGRMFRNEGMSPKHNPEFTSIELYQAYVDYEE
                     MMRLTENLFAYVAEKALGTTKINYQGTEIDLSPPWNRMDMADAVKEFVGVDFSKIESD
                     EEAREIAKAKGLDVDINMTRGHIISEMFEEFCESHLIQPTFITGHPVEISPLAKRNPD
                     DPRITNRFEAFINTWELANAFSELNDPIDQRERFEDQVKQKEHGDDEAHPMDTDFINA
                     IEVGLPPTGGLGIGIDRMIILLTNQPSIRDILLFPTMKPLGGEGSSNAEEGPVKETEE
                     KIDFSKVKVEPLFEEFVDFETFSKSDFRAVKVINCEEVPKSKKLLKFTLDDGSESERT
                     ILSGISEYYKPEDLVGKTLIAITNLPPRKMMGIESNGMVISAIHEEDGNERLNLLIVD
                     SHIPAGAKLY"
     gene            6012..>6418
                     /locus_tag="VFD79_07255"
     CDS             6012..>6418
                     /locus_tag="VFD79_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HZH93884.1"
                     /translation="MKILHITSQYPGKTGSGTYLKAMITEGHKEGYSQGLLGAVQEGL
                     DYSNPLLEYSQMVRFNTPDLPFPIMGMSDKMPYKSSIYKDMTLDQISRWEKVFAEKLR
                     ESLEEFKPDIILSNHLWLATSTVAQVVGDIPIFG"
BASE COUNT         2132 a         1180 c         1647 g         1459 t
ORIGIN      
        1 ccaaggtcag accggacaag gccatgggct atatggcggc ggaaagtgca agcacagagg
       61 aaagatcaca gggctgtgta ggctgtggga caggtgctag tgtgggaaag atacttggtc
      121 ctgagagttc aatgaagtca ggccttggaa gtgcaacagt cagggcagga gacctggttg
      181 tcagcgccct gatagctgtc aacagctttg gtgatgtttt ccataagggt gttcagatag
      241 caggcgtcta cgattacacc acggaccagc taatttccac tgaaaggata atgaaggaat
      301 ccactgacaa gatccagttt gcaaatacaa acacaaccat aggagtggtt gcaacaaatg
      361 ccttattgac aaaggcggag ggaaacaagg ttgcccagac agcccataat ggacttgcta
      421 ggtcaataaa tccagttcac acagctgttg atggggatac catttttgtc atggggacaa
      481 gcaaggtcca ggcagacctg aaccttgtgg ccatgcttgc tgcagaggct gttgagaagg
      541 ccgttataaa tgctgtaatg accgcaaaac cctatggaaa tatgaagacg gcaggggaaa
      601 taagtgaaaa ggtattgtaa ttaagaaggt cagcctatat aatgttaatg ttgaaaagct
      661 ggcatccaca tttgaggagc cagactgtag gaatgttaac ttattaaatc gactggtatc
      721 cagaggaacc agagcggagg gattattatg aaaaaaggaa gccgttttga catcaggaag
      781 ctgacaatca ttggagtgct gggtgcaatc tcagttgtgt tggggatgac ccccttgggt
      841 ttcattccag tgggacccac aagggcaacc ataatgcaca tacctgtaat aatcggggca
      901 atagtggagg gtccaatagt aggagccttg gtaggactga tatttggact gttcagtatt
      961 taccaggcaa ttactgcacc aaccccagta agctttgtat ttctaaaccc aatagtttca
     1021 gtgttgccta gagtattgat aggaataacc gcctactata cctacaaggg acttaacaag
     1081 cttgggacca agggaacaat tggaatgctg tacttgatct gggcagcaat aattgcatac
     1141 ctggcgagag ggatttatct aagcgtagct gattacacaa gcatctggcc attgcttatg
     1201 aatactggac tcttgatact gacagcgggc atggtatatc taacagccac caaattcaag
     1261 gacaaggcac tggacaccgt aatagctgcg gttgtgggaa ccatgaccaa cactgtaggt
     1321 gtactcacca tgatatatat attcttcggt gaaaggtttg tacaagccct tggacaggat
     1381 gttggtttgg caagaaaggt catagttgga ataggaataa ccaacggaat acctgagtca
     1441 ataatagcaa taataatagt gacaagcgta gtgagtgcgg taaaaagaag aagatagagg
     1501 gtgaacccat gttattggta atagacgtag ggaatacgaa catagtattt ggagcctatg
     1561 aaggagacaa gcttgtctac gactggagga tatccacaga caaggaaaag acatccgacg
     1621 agtacgggct tctgtttgat cagatgttta aataccacgg aatagttcca agcagtgtgg
     1681 acgatataat tatatcatct gttgttccca ctttgatgca taccctgccg gccatgagca
     1741 acaggtattt cggcaaagat ccaatagaga tcggcccagg agtaaagact gggatgaata
     1801 ttaagtacga taaccccaag gaagtgggtg cagataggat tgtaaatgca gttgctgcct
     1861 atgagaagta cggcggaccg gtaataatcg tagacttcgg tactgccatt accttctgtt
     1921 ttgttaacaa ggagggtgag tacctgggtg gagtgatcac accgggaatt aagatagctg
     1981 ccgaagccct tttcctaagg actgcaaagc ttcccaaggt tgaaataatt aagccggaaa
     2041 aggctatagg aaagactacc attggaagta ttcaatctgg actggtatac ggctttacag
     2101 gaatggtgga ttccctgatc gaaagaatgg tcaaggaaat gaaccacacc atggacgagg
     2161 taaaggttgt ggcgacggga ggcttctcca ccatgattgc gggggagagc aagtatattc
     2221 aatataacga taagtatcta acattggaag gattaaggat catatatgaa agaaacagta
     2281 agtagattat ccctgcttac tgtttcttta cgagaggtga agcatgaaga taggaagctt
     2341 taacatagag aacaaactga tgctggcccc aatggcagga gtgactgacc tggcctttag
     2401 aataatctgc aaggaaatgg gagtgggcct aacggtaaca gagatggtca gcgccaaggg
     2461 catgtactac aacgacaaga agacagacag cctgacccaa atagatccaa gggagaggcc
     2521 tgtggcactt cagattttcg gttctgatcc tgaaataatg ggagaagttg taagaaacaa
     2581 gctaaactac agggatgaca ttgacatcat tgacatcaac atggggtgtc cagctcccaa
     2641 aatagtcaag aacggtgatg gaagcgcact aatgagaaat ccaaagttgg ctgaggatgt
     2701 gatccgtagc gtcgctaagg aagccaataa gcctgtgacc gtaaaaatac gaaaaggctg
     2761 ggaccaaagc actgttaacg gagttgagat tgctaagata gccgaagctt caggcgcagc
     2821 agctataacc atacatgcaa ggacaagaga catgttctat gaaggaaagg ctgactggga
     2881 ttatataggc aggattaagg atgctgtggg gattccggtc ataggaaacg gggacatatt
     2941 tacaccccag gatgcaattg atatgatgga caggacaggg tgtgacggtg ttgcaattgg
     3001 aaggggagca atgggcaatc cctggatatt cagacagaca aaccaactca taaatggtga
     3061 tgagcctgtg aaaccatcct atacggaggt gcttgaaacc agcataaggc acatgaacat
     3121 ggcctgcgag ataaaagggg accgacttgg ggttagggaa atgcgcaaac agctggcctg
     3181 gtaccttaaa gggatgagaa actccaatga ggtaaaaaac cagatcaata ccatggagga
     3241 caggaaggag atagaattcc tccttagaac ctacatcaaa cacctgacag atggagataa
     3301 ttcccatagt gggctataat acttgacaac agggttttac ttgcatataa tatgttgcat
     3361 acaaagacgg aatatttgta atttgcagga tgaaaacgat atatttaagg taagaaaagg
     3421 ggagaccctt attttttata aatgtgagta tcaagagaag ctaccataat attgggtaaa
     3481 cgcttttatt attatattta aaggagagag tttaatggca gagaaggacg tttttttaac
     3541 ccgtgagggg ctggccaagc ttgaaaatga attggaagat gcaaaaatcg taaagagaaa
     3601 agagattgct gaaagaatca gaacagcatt ggattttggt gatatcagtg agaactcaga
     3661 gtatgaccag gccaagaacg agcaggctaa gcttgaggag agaatagcca aaatcgaagg
     3721 catcctaaga aatgcaaagc tgattgagga tgaggaaatc tcaactgata tcgttggcat
     3781 aggatcaagg gttgttgttc ttgatgttga atacgatgag gagatggaat acaccattgt
     3841 aggctcagct gaggccaacc catatgaagg taagatatca aacgagtcac cggttggaaa
     3901 agcccttttg gggagcaagg ctggagatgt tgtagatgta caggtacctg atggagtaat
     3961 aaaataccag atccagaaga ttgcccgata aattcaagga ggtatagtat gcaaacaggt
     4021 gaacaaagct taaacgaaat gctgcagata agaagagaga aattggctaa gttgatagaa
     4081 aaaggcaaga acccttttct aatagaaaaa tatgactaca gccaccacag ttctaatatt
     4141 aaggataact ttgaagagct tgaaggcacg gaagtatcag tggcaggaag gatcatgtcc
     4201 agaaggggac atggtaaggt aagctttata gacatacagg acagcgaggg aaggatccag
     4261 gtttttgcca agcaggacct gattggtgaa gaagagtatg aagacctgtc attcctggac
     4321 cttggagata ttatcggagc aaaaggtaca atattcaaga ccaagactgg agaggtatcc
     4381 gtaagggcca ctgagatagt gcttctcacc aagtcccttc agatacttcc agagaagttc
     4441 cacggtctaa aggaccagga ccttagatac agacaaagat atgttgacct gattgtaaat
     4501 cctgaggtaa aggatacctt tatacttaga accaaaataa tcaagggggt cagggaatac
     4561 ctggacgaca gaggattttt ggaggttgag actccaatac taagcaccat agcaggagga
     4621 gctaatgcca gaccctttga aacccaccac aacacccttg atgttgacat gtacctgaga
     4681 atcgccaacg aactttacct aaaaagactg attgttgggg gatttgaaaa ggtttatgaa
     4741 atgggcagga tgttcaggaa cgagggaatg agtccaaagc acaatcctga attcacaagc
     4801 attgagcttt accaggcata tgtagactac gaggagatga tgagacttac tgaaaacctc
     4861 tttgcatatg ttgcagaaaa ggcattgggt acaacaaaga tcaactacca gggaactgag
     4921 attgaccttt cacccccttg gaacagaatg gatatggcag atgcagtaaa ggaatttgtg
     4981 ggtgtagact tctcaaaaat tgagagtgat gaggaggcaa gggagatagc aaaagcaaaa
     5041 ggcctcgatg tggacattaa catgaccaga ggacacataa tctctgaaat gtttgaggaa
     5101 ttctgcgaaa gtcatctgat ccagcctact ttcataacag gacatcccgt tgagatttca
     5161 ccactggcaa agagaaaccc ggatgatcca agaattacga atagatttga agccttcata
     5221 aacacctggg agttggctaa tgccttctca gagcttaacg atccaataga ccaaagagaa
     5281 aggtttgaag accaggttaa gcagaaggaa catggagatg acgaagcaca tccaatggac
     5341 actgacttca taaatgcaat agaggtagga ttgccaccaa ctggaggtct tggcatagga
     5401 atcgacagga tgataattct tcttacaaat cagccaagca ttagggacat attgctgttc
     5461 ccaaccatga agccattagg tggagagggg agttcaaatg cagaggaagg ccctgtcaaa
     5521 gagactgaag aaaagataga tttttcaaag gttaaggtag aacctctctt tgaagagttt
     5581 gtggactttg aaaccttcag caagtcagat ttccgtgctg tcaaggtcat aaattgcgaa
     5641 gaggtaccca agagcaaaaa actattgaag ttcaccctgg atgatggttc agaatcagaa
     5701 aggaccattc ttagcggaat cagcgaatac tacaagccag aagaccttgt ggggaaaaca
     5761 cttattgcaa tcacaaacct accacccaga aagatgatgg gaatagaatc aaacggtatg
     5821 gtaataagtg caatccatga agaggatgga aatgagagat tgaatcttct gattgtagac
     5881 tcacatattc cggcaggagc aaaactatac taattggcaa tctgaagatt caaacatttc
     5941 accgacaaat agaagagatg cataagaggc ccccaaaatt gcgggtctct tttcatacac
     6001 ggaggtaatc tatgaaaatc ctgcacataa caagtcaata cccggggaag acaggtagtg
     6061 gaacctatct taaggcaatg ataaccgaag gccacaagga ggggtacagc caggggcttc
     6121 ttggagcagt acaggaaggt cttgactaca gcaaccccct gctggaatat tcccaaatgg
     6181 tccgtttcaa cacccctgac cttccatttc ccataatggg aatgagcgat aagatgccat
     6241 acaagagcag tatctacaag gatatgacct tggaccaaat atcaaggtgg gagaaggtgt
     6301 ttgccgaaaa actaagagaa agcctggaag agtttaagcc agacatcatc ctttccaatc
     6361 acctttggct tgcaacatct acagtggcac aggtggtagg agatataccc atatttgg
//
LOCUS       DASRQF010000272         2613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1363555, whole genome shotgun sequence.
ACCESSION   DASRQF010000272 DASRQF010000000
VERSION     DASRQF010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363555"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="VFD79_07260"
     CDS             complement(<1..1190)
                     /locus_tag="VFD79_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014969027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HZH93885.1"
                     /translation="MERNTYIDNVDVDKALETYLEALSIQGESEEIPVYDSLGRITWG
                     AIYAKVSSPAYNASAMDGIAVTSKRTEGANESKPLTLVEEVDFKYVNTGNPIKDPYDA
                     VIMIEDVLQGEEGKVQILRPAYPYQHVRQIGEDIVATEMILPSRHRIRPMDIGALISG
                     GIESVNVYKSPRIGIIPTGSEIVEDASQVREGTIIDSNSHVFKGLVESLGGMAKRYPP
                     MEDDYEKLKDLISQAVEENDMIIINAGSSAGTKDFTVNLIQELGKVVVHGVALKPGKP
                     TILGIIKGKPVIGIPGYPVSAFMVFDVFAKPIILKYLGDQGEIYETTEAFLSRRMVSS
                     LKNKEIVRVTLGKVKDRLIATPLSGGAGVSMSLVKADGLGIIPRNVEGYEAGDRIRVE
                     LLKP"
     gene            complement(1207..2433)
                     /gene="glp"
                     /locus_tag="VFD79_07265"
     CDS             complement(1207..2433)
                     /gene="glp"
                     /locus_tag="VFD79_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HZH93886.1"
                     /translation="MEFFRVVSVNDARDLIKNEFGNYLMGSEEVSLVQAQGRVLSDDV
                     VSDINVPEFDRSTVDGYAIQVEDSHGATESIPSILEVLGEVLMGETPNYEVKTGQAVY
                     VPTGGMMPMGATGMIMIENTEKMDQETVLLFKPISKGENMVFKGEDIKTGQVALQAGR
                     RIGPNEIGVLASLGIKRVKVIKRPQVYILSTGDEVIDLDEELQMGKIRDINSYALSAM
                     IKEMGAEVAGREIVKDNYELLMSGVKNALKEADIVLLSGGSSVGTRDYTHKVITDLKG
                     KGVLAHGLSIKPGKPTIIGDGQGKLIVGLPGHPVSSIVVFKAIVQPYIQSLLKAPSHS
                     RMVEAITTHNFPSSPGKETYHMITLKEEDGKILATPSFGKSGMITLLSRSNGYIVIAE
                     HEEGIDKGETREVYLF"
     regulatory      complement(2481..2595)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
BASE COUNT          627 a          696 c          492 g          798 t
ORIGIN      
        1 attggcttta agagctccac ccttatcctg tcccctgcct catagccttc aacatttctt
       61 ggtatgatcc caaggccatc ggcctttacc agactcatgc tgactcctgc tccacctgat
      121 aatggagttg ctataagcct gtcctttacc ttgccaagtg tgacccttac aatctcctta
      181 ttcttaaggg aagaaaccat tcttctactt agaaaggctt ctgttgtttc atatatttct
      241 ccctgatctc ccaaatactt aagtatgatt ggttttgcaa aaacatcaaa gaccataaac
      301 gcagaaaccg gatagcctgg aattccaatt acaggcttgc ctttaattat ccctagaatg
      361 gttggttttc cgggctttaa tgccactcca tggacaacta ccttcccaag ttcctgaata
      421 aggttcacgg taaagtcctt tgttccggct gatgagccag catttatgat gatcatgtca
      481 ttctcttcca cagcttgtga aatcagatct ttaagctttt cataatcatc ttccataggg
      541 gggtatcttt tcgccattcc acctagggat tcaaccaaac ctttgaaaac atgggaattt
      601 gaatctatga tggtcccttc ccttacctgg gatgcatcct ccacaatttc agacccggtg
      661 ggtattatcc cgatccttgg gctcttataa acatttacac tctcaattcc accggaaata
      721 agtgctccta tgtccatggg acgtattcta tgcctggatg gaagaatcat ctctgttgca
      781 acaatgtcct ctccaatctg cctcacatgc tgatagggat aggccggtct aaggatctgc
      841 accttgcctt cctctccctg gagtacatcc tctatcatga tgacagcgtc ataggggtcc
      901 ttgattggat ttcccgtgtt aacgtattta aaatccacct cttccacaag agtcaatggc
      961 ttgctctcat tggccccctc tgtcctcttg gaggtaaccg cgatcccatc cattgctgag
     1021 gcgttgtatg caggtgagga taccttggca tatattgctc cccaggttat ccttccaagg
     1081 gaatcataaa ctggaatctc ctcactttcg ccctggatgg aaagcgcctc cagataggtt
     1141 tccaaagcct tgtccacatc tacattgtct atataggtat ttctttccat aatcacatct
     1201 ccctcgttaa aacagataaa cctctctggt ttcgcccttg tctattcctt cctcgtgttc
     1261 agctattaca atatagccat ttgacctgga aagaagggtt atcatgcctg actttccaaa
     1321 gcttggtgta gcgaggattt tcccatcctc ctcctttagt gttatcatat ggtaggtctc
     1381 ctttccggga gatgacggga agttgtgagt cgtaatggcc tccaccatcc ttgagtggga
     1441 cggtgcctta agaaggctct ggatgtaggg ttgaactatt gccttgaaca ctacaattga
     1501 tgaaacaggg tgtccaggta gacctacaat aagcttccct tgtccatcac caataatcgt
     1561 gggttttccc ggttttatgg aaagtccatg agccagcacg ccctttccct ttaggtctgt
     1621 tatgaccttg tgggtataat ccctggtacc aacagagctt ccaccggaaa gaagtacaat
     1681 atccgcctcc ttaagggcat tttttacccc actcataaga agttcataat tgtccttgac
     1741 gatttcacgg cctgccacct ccgcccccat ttcctttatc atggctgaga gggcgtatga
     1801 atttatatcc ctgattttac ccatttggag ctcttcatca aggtcgatca cctcatcgcc
     1861 agttgagaga atatagacct ggggtctctt tattaccttg acccttttta ttccaagact
     1921 tgccagaact cctatttcat tgggtccaat cctccgacct gcctgtaggg caacctggcc
     1981 ggtttttatg tcttcaccct tgaaaaccat gttctcgccc ttgctgatgg gcttaaaaag
     2041 aagcaccgtt tcctggtcca tcttttccgt attctctatc attatcatgc cagttgctcc
     2101 cattggcatc atgccccctg taggcacgta aacagcctgt ccggtcttga cttcataatt
     2161 tggagtctca cccatcagaa cctcgccaag gacctccagg atgcttggta tggactctgt
     2221 ggcaccatgg ctgtcctcta cctggattgc atagccgtca actgttgacc tgtcaaactc
     2281 aggtacattt atatccgaaa caacatcatc cgagagcact cttccctgag cttggaccag
     2341 gctgacctcc tcggacccca taaggtaatt tccaaattca ttttttataa gatccctggc
     2401 atcattaact gacaccaccc taaagaattc catgtaatac cacctttcat atgcgaaaaa
     2461 aaaacaacct gtaaggaagg ttgtcaaata aacatcttcc tttccaaacg ggaggcttca
     2521 ggatttctcc tttacccaac ggcattgctt catggcattc gctttagaaa tcaatgctcg
     2581 gaaaatttgt atagatattc attttttctc ccc
//
LOCUS       DASRQF010000273         9290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_223785, whole genome shotgun sequence.
ACCESSION   DASRQF010000273 DASRQF010000000
VERSION     DASRQF010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 9290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9290
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223785"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="VFD79_07270"
     CDS             <1..1108
                     /locus_tag="VFD79_07270"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HZH93887.1"
                     /translation="GGEIHNGKIYGRGTMDDKGPAVIALYAMKALADSGVKPGRKIRL
                     ILGANEESRWECMDHYFKNEEPPTMAFTPDADFPVIHGEKGITGFDLKFPYKGTKGCD
                     VTLNDIKGGNAPNMVPDKAWANLSVKDKNLFRSQFNDYMKDKSISIDLEEGDKGFKVT
                     ALGKSAHGSTPAKGENAISYLMDFLGFVYSGQCPVCRFIDFYNERIAFKHNGEGIGCA
                     LEDDLSGKLDFNPGLISYDGNHIKLSVNVRYPISSSADEVFAGIRENIKDTPIELVEG
                     ALDSRPLYVAKDNELVETLMKVYREQTGDLDSEPITIGGGTYARAMKNAVAFGPVFPG
                     QEELAHQKDEHITIENVVKLTEIYSHALYELVQQ"
     misc_feature    1161..1303
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1282..1839
                     /gene="infC"
                     /locus_tag="VFD79_07275"
     CDS             1282..1839
                     /gene="infC"
                     /locus_tag="VFD79_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HZH93888.1"
                     /translation="MSAFLIYFYNYRRCRIIKELQINEEIREKEVRLIDVDGSQLGVV
                     PIKKALDIAFERRLDLVNVAPTAKPAVCRVMDYGKYKYEMAKKEKEARKNQKIINIKE
                     IRLTPNIETHDLNVKANRAIDFLKDGDKVKVSVRFRGREMGHTEMGKVVLDEFAQLTS
                     EVGKIEKEAKLEGRNMVMFLMPKAD"
     gene            1858..2049
                     /gene="rpmI"
                     /locus_tag="VFD79_07280"
     CDS             1858..2049
                     /gene="rpmI"
                     /locus_tag="VFD79_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HZH93889.1"
                     /translation="MAKMKTHRGSAKRFKRTASGLLKRFKAYKSHKTGKKSPKRVRNL
                     RKGALVSKGDQKRIDSMLP"
     gene            2081..2431
                     /gene="rplT"
                     /locus_tag="VFD79_07285"
     CDS             2081..2431
                     /gene="rplT"
                     /locus_tag="VFD79_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HZH93890.1"
                     /translation="MARVKRGVNAKKRHKRVLKAAKGYYGAKSKLFKPANQAVMKSLN
                     YAFIGRKQKKREFRKMWIARINAAARQNGMSYSKFISGLKKSGIEVNRKMLSEMAIHD
                     SEGFSKLVEIAKEA"
     gene            2504..3847
                     /locus_tag="VFD79_07290"
     CDS             2504..3847
                     /locus_tag="VFD79_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HZH93891.1"
                     /translation="MSYIRERLYLFELNPPRVLALGFLGLILFGSVLLNLPVASADGN
                     SIGFLDALFTSASAVCVTGLIVVNTATYWSVFGKVVILFLIQIGGLGIMTMATMVALI
                     MGKKISLKERLIIKEQMNQETMSGMVKLTLYVILLTFAIEAIGAIILSFRFVPEYGLT
                     KGIWFSVFHSISAFCNAGFDITGNSLMSYTDSPLTIMTLSGLIIIGGTGFSVLIDLAK
                     HRRWVRLSLHTKLVITMSLGLILLGTIFFFVLEYSNPATIGQMPLGEKLSAAFFAAVV
                     PRTAGFNSIDTAALRESSVFLTIILMFVGGSPGSTAGGVKTATIGVVLMATISIIRGD
                     KDIEIYEKRLNQETVFKSLAIVTVGMGMIVLISFLLTITESKNFLDVLFEATSAFATV
                     GLSRGITSDLTTIGKIIIIGTMYAGRVGPLTMAYAIGYNKKHKRYRYSEGHISVG"
     gene            3859..4515
                     /locus_tag="VFD79_07295"
     CDS             3859..4515
                     /locus_tag="VFD79_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HZH93892.1"
                     /translation="MKSFAVIGCGRFGSAVAKTLFELGNEVLAIDGDMDVVDNIAGHV
                     THAMQADVMDEGVLKELGLGNFDVVVISIGSDLQASIMATLIVKELGVGKIVAKAQSE
                     LHGKVLKKIGADKVIFPERDMGIRVAHNLASNSIVDYIELSPDYSIMEIEAVSKWYDK
                     TLEELRLRNKYHVTVMAIKRGEDVSVSPGPTYKIHRGDILVALGSTDAINDIAEKAGE
                     "
     gene            4517..5305
                     /locus_tag="VFD79_07300"
     CDS             4517..5305
                     /locus_tag="VFD79_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HZH93893.1"
                     /translation="MLEVTSPKNPLIKEVRSLDRRKYRWGLKKFLVEGVKMVDEALGN
                     KDQAIKILFTDSLTSTEQGKNLFNKLAAMDNSVYLQEELFRSISNMENSQGVLATVGF
                     TPKGIEHIPDEGILLYLDGLQDPGNLGTIIRSADAFGISGLVLGDNCVDPYNPKVVRA
                     TMGSIFRVPLYFQNNNEDFLLNLSAGRRILATSLEDASPLSDIIFSNRDVIVIGNEGH
                     GVSPDILKLSDIRIVIPMVGNAESLNAGVAASIIMYETSRKLIK"
     gene            5387..6409
                     /gene="pheS"
                     /locus_tag="VFD79_07305"
     CDS             5387..6409
                     /gene="pheS"
                     /locus_tag="VFD79_07305"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HZH93894.1"
                     /translation="MKERLEALRDAAFKEIQDNIDLNELEELRVKFLGKKGEITKILR
                     DMGKLSAEMRPVVGQMANEVRADLENKISETKEILKTKAKEQRVASETIDITVNTNQI
                     KRGHRHPLIQTAEELEDLFISMGFSVVTGPEIETVENNFDALNSPDNHPSRDLSDTFY
                     INEDMLLRTHTSPVQIRSMKKYGAPLRIVSAGRTFRYDDVDDTHSPMFHQLEGLVVDE
                     NISMANLIHTLDIFIKELFGEDMKTRYRPHHFPFTEPSAEVDVTCIECRGEGCPACNF
                     TGWSMELLGCGMVHPQVLENCGIDSEKYTGFAFGMGIDRIAMVKYGIKDIRLLFENDN
                     RFLKQF"
     gene            6424..8817
                     /gene="pheT"
                     /locus_tag="VFD79_07310"
     CDS             6424..8817
                     /gene="pheT"
                     /locus_tag="VFD79_07310"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HZH93895.1"
                     /translation="MLLPVNWLKNYVDLSISSKEIADGLTYSGSHVESITSMNKGIEN
                     VVVAKLESIETHPNADKLLVCKASTGPNTYTVVTGAKNLKSGDYVPLALPGAKLPGGI
                     EIGITDFRGVESQGMLCSLKELGYADNVIPKEMRDGIFVFDKDYELGQDVVNLMQLDD
                     DIIEFEITPNRPDCLSIYGMAREASATFNGELQEPEIEIENEVDDIHEYTNGIEVKTE
                     ACRRYYSRVIKDVVIKPSPLWMQTTLMNAGVRPVNNMVDITNYVMLELGEPLHAFDLD
                     MLGGRKIIVRQAEAGEKLTTLDDVERILDQNDVVIADENTAIGLAGIMGGLDSEITKE
                     TKTVLLEGANFSPRHVRLTSKKLALRSEASTRFEKGIDPNLCSQAVERVCELVEKTGA
                     GKVVKGVIDVDQTSGNPHSILMRPERARMLLGVEIPDEDLIDYLERLRFKTTVKEGNI
                     IAEVPSYRDDVKVEADLIEEAGRLYGFHNIESKPLKGGLTRGEKPYNKQIKARVKSVL
                     QGIGYNEVMTYSFISPKAYDKLNLPEDAPERNYIRLMNPLGEDYSTMRTTLMSNMMDL
                     LGRNENKGIEEVFAYEIGYTFIPKSLPVVDLPEEKQVLSIGFYGDKDFYYMKETLEMV
                     FSRLGIKNLDYRRLTDNYSFHPGRAARVYSGDDELGVFGEVHPDVAGNYELKNRPYVA
                     TINFDKVVELTDLDIKYKPLPKYPSMTRDIAVVVDEDIMVGELEEVIIHHGKDLVESI
                     DLFDIYRGNQVEKNKKSVAFSIVYRSYEGTLTDSVVNKIQTSIIEDLENKFDAKLRS"
     gene            8881..>9290
                     /locus_tag="VFD79_07315"
     CDS             8881..>9290
                     /locus_tag="VFD79_07315"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HZH93896.1"
                     /translation="MTDKKKINVLIDGRNFTVVGAEDERYVRELAAFVDSGIKKMSNK
                     NERLSPTMAATLTAFNIADELFKLQDEHKELKKRSKEPLEKYDSLKKEVQDANAKIRE
                     METKSLEYQDEVIKTKLSKEELFGEINNLKENVK"
BASE COUNT         3050 a         1550 c         2273 g         2417 t
ORIGIN      
        1 tggtggagaa atacacaatg ggaaaatata cggtagaggt accatggatg acaagggccc
       61 tgcagtaatt gcactttatg ccatgaaggc attggcagac agcggtgtta agccaggtag
      121 gaagatcagg ctgattctcg gggcaaacga ggagagcaga tgggagtgta tggaccatta
      181 tttcaagaat gaagagccac caaccatggc atttactcct gacgcagatt ttcctgtgat
      241 ccatggcgag aagggaataa caggctttga ccttaagttc ccatacaagg gtacaaaagg
      301 ctgtgatgtc acccttaatg acattaaggg aggaaacgca cccaatatgg taccagataa
      361 ggcctgggcc aatctttctg ttaaagacaa gaaccttttc cgctctcagt tcaatgacta
      421 catgaaggac aaaagtattt ccatcgacct tgaggaaggg gataaaggat tcaaggtaac
      481 ggcactaggt aaatctgccc atggaagtac tcctgcaaaa ggggagaatg ccatcagcta
      541 cttgatggat tttctgggat ttgtttattc agggcaatgt cctgtttgca ggttcataga
      601 tttctacaat gagagaattg cctttaagca caatggtgaa ggaataggct gcgcccttga
      661 agatgatctt tcagggaaac tggatttcaa ccctgggcta attagctacg atggcaacca
      721 catcaagtta tctgtcaatg taaggtatcc tatcagcagt tctgctgatg aagtttttgc
      781 tggaatcaga gaaaacatca aggacactcc cattgagctt gtggaaggag cattggattc
      841 cagacccttg tatgtagcaa aagacaatga gctggtggag accttgatga aggtctacag
      901 ggagcaaacc ggggacttgg attctgaacc cataacaatt ggagggggaa cctatgcacg
      961 agccatgaag aacgcagtgg cttttggacc ggtattccct ggacaagaag agttggcaca
     1021 tcaaaaggat gaacacatca caattgagaa tgtagtaaag cttactgaga tttattcaca
     1081 tgcactttat gaactcgtgc aacaataatg tttgacaaac acgtaatgat gacatataat
     1141 ggttaaggaa ttgaatatat aattcaaagt agaagttccg cttctcacct tatagccgag
     1201 gctcataagg ttgacagaat tatcctgtgg atttattagt gcacgggact tgccatgcta
     1261 tggtatttga gcggacattc tatgtccgct tttttaattt atttttataa ctacaggagg
     1321 tgtaggatta ttaaagaact tcaaatcaac gaagagataa gggaaaaaga agttagactt
     1381 attgatgtgg atggtagtca gctaggtgtg gtgcctataa agaaggcatt ggatattgca
     1441 ttcgagagaa gacttgattt ggtaaacgtt gctccaactg caaaaccagc agtatgtaga
     1501 gtaatggact acggaaaata caagtacgaa atggctaaaa aggaaaaaga agccagaaag
     1561 aatcaaaaga ttatcaacat aaaggaaata aggttaacac ccaatatcga aactcatgac
     1621 ttgaatgtta aggcaaaccg agcaatcgac ttccttaagg atggagataa ggtaaaggtt
     1681 tcagttaggt tcagaggtag agagatgggc catactgaaa tgggtaaagt ggtactggac
     1741 gaatttgccc agctgacaag tgaagttggg aaaatcgaaa aagaagcaaa gcttgagggc
     1801 agaaacatgg taatgttcct tatgcccaaa gcagactaag ttaggaggaa ttggacaatg
     1861 gcaaagatga aaacacatag aggctcagca aagaggttca agagaactgc ttcaggtcta
     1921 ctaaagagat ttaaagctta taagagccat aagactggaa agaaatcacc taaaagagtt
     1981 agaaacctaa gaaaaggtgc actggtaagc aagggagacc aaaaacgaat cgattcaatg
     2041 ctaccatagt attgagaaac ttatatagga ggcgtgtaca atggcaagag taaaaagagg
     2101 cgttaatgcc aagaagagac ataagagagt attaaaggct gcaaaaggat attatggtgc
     2161 aaagagcaag cttttcaagc cagcaaatca agctgttatg aaatcattga actatgcatt
     2221 tatcggacgt aagcagaaga agagagagtt cagaaaaatg tggattgcca gaataaatgc
     2281 agcggcaaga caaaatggaa tgagctacag caagttcata agcggactta aaaaatcagg
     2341 aatcgaagta aacagaaaga tgctttcaga aatggctata catgattcag aaggtttttc
     2401 taaactggta gaaatagcaa aggaagcgta aatgcaaaag ttccattagg aacttttttt
     2461 gcatttatag ccctactttc tacatacata atggaggact attatgtcat acataagaga
     2521 gagattgtat ttatttgaat tgaacccacc tagagttctt gctctaggat ttcttggctt
     2581 gatacttttt ggttctgtat tattgaatct tccagtggct tcagcagatg gcaatagtat
     2641 aggtttcttg gatgctttgt tcacttcagc ctctgctgtt tgcgtgactg gtctgatagt
     2701 tgtgaacaca gcaacttatt ggtcggtgtt tggcaaggtc gtaatacttt ttctgataca
     2761 gattgggggc cttggaataa tgaccatggc aacaatggta gccttgatca tggggaagaa
     2821 gatatcccta aaggagaggc tgatcatcaa ggagcagatg aatcaggaga caatgtctgg
     2881 catggtaaag ctaaccctct atgtaatcct attgacattt gccatagagg caataggtgc
     2941 aataatcctg tcttttagat ttgttccaga atatggtctt acaaagggga tttggttttc
     3001 tgttttccat tccatatcgg cattctgcaa tgccggattt gatattactg gaaacagcct
     3061 gatgtcatat actgattcac cacttacaat tatgacatta agtggtctta taataattgg
     3121 agggaccgga ttctctgtac tgatagacct ggcaaagcac agaagatggg taagacttag
     3181 tctccatacc aagctggtaa taacaatgtc tttaggccta atactcttgg ggacaatatt
     3241 cttttttgtc ttggagtatt ctaatcctgc gacaattggt cagatgccat taggggagaa
     3301 gctatcagca gctttttttg cagcagttgt tccaaggact gcaggattca attcgattga
     3361 tactgccgct ttaagggaaa gcagcgtatt cttaacgata atcctgatgt ttgttggggg
     3421 ttctccagga tcaactgctg gaggagtcaa gactgcaacc ataggtgtgg tactgatggc
     3481 aacaatcagc ataataagag gcgacaagga cattgaaata tatgagaaaa ggcttaatca
     3541 ggagacagtg tttaagtcct tggccattgt aaccgtggga atgggaatga tcgtcttaat
     3601 atctttttta cttacgataa ctgaaagcaa gaacttcctg gatgtgttat ttgaagccac
     3661 atctgcattt gcaacagttg gactctcaag agggataact tctgatctta ctacaatcgg
     3721 taagattatc ataattggca ccatgtatgc tggaagagtt ggaccactta caatggctta
     3781 tgccatagga tataataaaa agcataaaag atacagatat tcagaagggc atatatcagt
     3841 tggataggag gaagaggaat gaagtctttt gcggtgatcg gttgtggcag gtttggttcg
     3901 gcagttgcaa agacactttt tgaacttggc aatgaggttt tggcaattga tggggacatg
     3961 gatgtagtgg acaacatagc aggacatgta acccatgcaa tgcaggctga tgtaatggat
     4021 gagggtgttt tgaaggaact gggactcgga aatttcgatg ttgtggttat aagcatagga
     4081 tctgatttgc aggcatccat aatggctacc ttgatagtca aggagttggg tgtaggcaag
     4141 atagtggcta aggctcaatc cgagctacat ggaaaggttc ttaagaagat tggtgcggac
     4201 aaggttatat tccctgaaag agatatgggg attagggtgg ctcataatct ggcatctaat
     4261 agcatagtcg actacattga gctttcacct gactacagta ttatggagat tgaggccgtt
     4321 tcaaaatggt acgacaagac tctcgaggag cttagactac gcaataagta tcatgtcaca
     4381 gtgatggcca tcaaaagagg agaggatgta agtgtctctc caggaccaac atataagatc
     4441 catagaggcg atattcttgt tgcgttgggg tctactgatg cgataaatga tattgctgag
     4501 aaagcaggtg agtaatatgc tggaggtaac ttctcccaag aatcccttaa taaaggaggt
     4561 caggtccctc gacagaagga aatatcggtg ggggcttaaa aaattccttg ttgagggagt
     4621 aaaaatggtg gatgaagccc ttggtaataa ggaccaggcc ataaaaatac tttttacgga
     4681 ttctctaacc agtacagagc aagggaaaaa cctctttaac aagctggctg caatggataa
     4741 ttcagtctat ttgcaggaag aactatttag gagtatttca aacatggaga attcacaagg
     4801 ggtattggca accgttggct tcacgccaaa gggtattgag catatacctg atgaggggat
     4861 attattatac cttgatggcc ttcaggatcc aggtaatctt ggaacaataa tcaggtctgc
     4921 agatgcattt ggaataagtg gattagtcct tggagataat tgtgtcgacc cttacaaccc
     4981 aaaggttgta agggcaacaa tgggatcgat attcagggtc cccctatatt ttcaaaataa
     5041 caatgaagac tttcttttaa atctatctgc aggcaggaga attcttgcta cttctcttga
     5101 agatgcaagt cccttaagtg acataatctt cagtaacagg gatgttattg ttattggcaa
     5161 tgaagggcac ggagtctcac ctgatatctt aaagctgtct gacattagga ttgttatacc
     5221 aatggtgggg aatgcagaat cactaaatgc aggggttgca gcatccataa taatgtatga
     5281 gaccagtcgg aaacttatta aataggttga gttttcgcca ttgtggtgct ataatttagt
     5341 aaataatctg aaatttcatt ataaattaat ttgaaaggag cctattatga aggagagatt
     5401 ggaagccttg agggatgctg ccttcaagga gatacaggac aatatagatt taaacgagct
     5461 ggaggagctt cgtgttaagt ttcttggcaa gaagggtgaa ataaccaaga ttctaagaga
     5521 catgggcaag ctttcagctg agatgagacc agttgtcggg caaatggcta acgaggtgag
     5581 agctgatctt gagaataaaa tcagtgaaac aaaggagata cttaagacaa aagccaagga
     5641 acagagagtt gcttccgaga caatagatat aacagtaaac accaatcaga taaagagagg
     5701 tcacagacac cccttgatac aaactgcaga ggaactggag gatttgttta tatcaatggg
     5761 attctcggta gtgacagggc cagaaattga aacagtggaa aataacttcg atgccttgaa
     5821 ctcccctgac aaccatccat caagagacct ttcagataca ttttatataa atgaggatat
     5881 gcttttaaga acccacacat ctcctgtaca aataagatcc atgaagaagt atggggctcc
     5941 attgaggatt gtatccgcag gtaggacctt ccgctatgac gatgtggatg acactcattc
     6001 accaatgttc catcagcttg agggcttggt tgttgatgaa aacatatcca tggccaacct
     6061 gattcacaca ttggacatat tcatcaagga gctttttgga gaggacatga agaccaggta
     6121 ccgcccacac cattttcctt ttacagagcc cagtgcagag gttgatgtaa cctgcataga
     6181 atgtagaggg gaaggttgtc ctgcatgtaa tttcactgga tggagcatgg agcttttagg
     6241 ttgcggaatg gtacatcccc aggtgcttga gaattgtgga atagattctg agaaatacac
     6301 tggatttgct tttggaatgg gaatagaccg aatagcaatg gtgaaatacg gaataaagga
     6361 tattagactc ttatttgaaa acgacaatag atttcttaaa caattctagg gggtattgaa
     6421 aggatgttat taccagttaa ttggctaaaa aactatgttg atctgagcat atcttcaaag
     6481 gagatagctg atggtctgac ttattcagga tcacacgttg aatccataac aagcatgaac
     6541 aagggaattg aaaatgttgt ggttgcaaaa cttgaaagca tagagacaca tccaaatgct
     6601 gataagcttt tggtatgcaa ggcaagcaca ggacctaata cgtacacagt agtaactgga
     6661 gcaaagaacc tgaagtctgg tgattacgtg ccattggcct tgccaggtgc aaagctgcca
     6721 ggtgggattg aaataggtat cactgatttt aggggagtgg aatcacaagg gatgctttgc
     6781 tcattaaagg agcttggata tgctgataat gtcataccca aggaaatgag ggacggaata
     6841 ttcgtctttg ataaggacta tgaattagga caagatgtag taaaccttat gcagttggac
     6901 gatgatataa ttgagttcga aataactcca aacaggcctg actgcctcag catttatgga
     6961 atggcaagag aagcatctgc gacctttaat ggggaactgc aggagcctga gatagagatt
     7021 gagaatgaag ttgatgatat tcatgaatac acaaatggaa tagaggtcaa gacagaagcc
     7081 tgtagaagat actattctag ggtcataaag gatgttgtaa ttaagccatc accattatgg
     7141 atgcaaacaa ccctaatgaa tgcaggtgtt agacctgtaa acaacatggt tgacattacc
     7201 aactacgtaa tgcttgagct gggtgaaccc cttcatgcct ttgaccttga tatgcttggt
     7261 ggaaggaaaa taattgtaag acaagcagaa gctggagaaa aattgacaac cttagatgat
     7321 gtcgaaagaa ttctggacca gaacgacgtt gtaattgccg atgagaacac tgcaattggc
     7381 ttggcaggaa tcatgggtgg acttgacagt gaaataacaa aggaaaccaa gactgttcta
     7441 ctggaaggcg ccaacttcag tccaagacat gttcgtctta catccaagaa attggcctta
     7501 agatctgaag catccacacg atttgaaaag ggaattgacc ctaatctttg tagccaggcc
     7561 gtcgaaagag tatgcgaact tgttgaaaaa acaggcgctg gaaaggttgt aaagggtgta
     7621 atagatgttg atcagactag tggaaaccct cattccatat taatgagacc ggaacgtgca
     7681 aggatgcttt taggagtgga gataccagac gaggatctaa tagactatct ggaaaggttg
     7741 agattcaaga caactgtcaa ggagggtaac ataattgcag aggtaccatc ctatagagat
     7801 gatgtaaagg ttgaggctga cctgatagag gaagctggaa ggctgtacgg gttccacaac
     7861 atagagagca aaccgcttaa aggaggcttg accagagggg agaagcccta taacaagcaa
     7921 attaaagcaa gggttaaatc agttcttcag ggaattggct ataatgaggt tatgacctat
     7981 tcatttatca gtccaaaagc atacgacaag cttaatctac ctgaggatgc acctgaaaga
     8041 aactatatca gattgatgaa tccacttgga gaagattaca gtacaatgcg caccacattg
     8101 atgtccaaca tgatggatct actgggaaga aacgaaaaca aaggaattga ggaagtcttt
     8161 gcctatgaaa tcggatacac ctttatcccc aagtctttgc cagtggtaga ccttcctgag
     8221 gaaaagcagg tcctgtccat tggtttctat ggagacaagg atttttatta catgaaggag
     8281 acacttgaaa tggtctttag tcgactggga atcaaaaatc tagactatag acgtttgaca
     8341 gacaattatt cattccatcc tgggagagct gcaagagtat attcaggaga tgatgaactt
     8401 ggtgtatttg gagaggtcca tccagatgtt gcgggtaact atgagctgaa gaacagacca
     8461 tatgttgcaa ccataaactt tgacaaggtg gtagagctga cagatttgga cattaaatac
     8521 aagccattgc ctaagtatcc atcaatgaca agggatattg ctgttgtagt tgatgaggat
     8581 ataatggttg gagaacttga agaagtgatc attcaccatg gcaaagacct tgttgaatcc
     8641 atcgatcttt tcgatatata cagaggcaat caggttgaaa agaacaagaa gagtgttgcg
     8701 ttctcaatag tatacagatc atacgaggga actcttactg attctgtagt aaataaaatt
     8761 cagaccagca taattgaaga ccttgaaaat aaatttgatg caaagctaag gagctagggg
     8821 agggtttcct cccctatcat taagcgtgtg aagataccgg actgtttaag gaggatggtt
     8881 atgacggata aaaaaaagat taacgtgtta attgatggtc gtaactttac agtagtaggt
     8941 gccgaggatg aaaggtatgt tagagaattg gcggcttttg tggatagtgg catcaaaaag
     9001 atgtccaaca agaacgaaag acttagtcca acaatggccg caacccttac tgcattcaat
     9061 attgcggatg agcttttcaa gctccaggat gagcataagg aattaaagaa aaggtcgaag
     9121 gaacccttgg agaaatatga ttcccttaaa aaggaagtcc aggatgctaa tgcaaagata
     9181 agggaaatgg agaccaaaag ccttgaatat caggatgagg ttataaaaac caaattgtcg
     9241 aaggaagagc tttttggtga aataaacaat ctcaaagaga atgtaaaaag
//
LOCUS       DASRQF010000274         2421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_661013, whole genome shotgun sequence.
ACCESSION   DASRQF010000274 DASRQF010000000
VERSION     DASRQF010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661013"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="VFD79_07320"
     CDS             complement(<1..1241)
                     /locus_tag="VFD79_07320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZH93897.1"
                     /translation="MDELRLIEEIIEEKEKTLVDASRKIWEAAELPYMEEKSARILMN
                     ILRDEGFDVVEGVSDMPTAFIGRFGTGKPVAGFLGEYDALDALSQRAGITEKSPLVKD
                     GPGHGCGHNLLGTGSLGAAIALKEFLKRTGREGTVIYYGTPAEEGAGSKQFMARDGVF
                     DEADFIYTWHPATKNEVQSDRTVAIMGANFRFKGTTAHAGGSPHLGRSALDAAELMSV
                     GVNYLREHMIDQARIHYAYVDAGGVSPNVVQDRSLVKYEVRAPKVTQMRDLFERVVDV
                     ARGAALMTGTSVEYDITMAFSDYISNAVLAKIGDECFEEVGPPRWDEEDYRLAEGFLR
                     TYDKATINSIMEDLEEIYGTEGIEEVLRAPLDSKVHPWKEGNRTYRSGSTDVGDVSYV
                     VPTLNFNVATACLGNVGHTWQ"
     gene            complement(1254..1976)
                     /locus_tag="VFD79_07325"
     CDS             complement(1254..1976)
                     /locus_tag="VFD79_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004825014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HZH93898.1"
                     /translation="MIRVENLDKSFGELKVLKDINESIYEGEVISIIGPSGSGKSTFL
                     RCLNLLEKPTGGKVIFKGIDLADKKVNVDLHRQKIGMVFQLFNVFPHLTVLENITLAP
                     ILEKKVPRKEAEKKADDLLKRVGLADKKDEYPTRLSGGQKQRLAIVRALAMEPDVMLF
                     DEPTSALDPEMVKEVLDVIKSLVETGMTIVIVTHEMGFAREVSDRVLFMDEGIIQEQG
                     SPDDIFNRPKNPRTIEFLSKIL"
     gene            complement(1984..>2421)
                     /locus_tag="VFD79_07330"
     CDS             complement(1984..>2421)
                     /locus_tag="VFD79_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HZH93899.1"
                     /translation="WILLPKISPVTLSDTACIVIALIVNASSYVAEIIRAGIQAVDKG
                     QTEAAKSLGMSNRNTMRRIILPQAIKNILPALGNQYINMVKQTSLASVFFVQELMTSY
                     RTVQSATFLAIPSLVIVGIIYLVVTTVLSKGLDIFERRLQANE"
BASE COUNT          568 a          669 c          478 g          706 t
ORIGIN      
        1 atctgccagg tatgcccaac atttccaagg caggctgtgg caacgttgaa attcagggtt
       61 gggacgacat agctcacatc ccctacatcc gtagacccag accggtaggt tctattgccc
      121 tccttccagg ggtgtacttt cgaatcaagg ggtgccctaa gcacttcctc aataccttca
      181 gttccatata tttcttccag gtcctccatg atggagttta tcgtggcctt gtcataggtt
      241 ctaagaaaac cttctgcaag cctgtaatcc tcttcatccc atcttggtgg tcccacctcc
      301 tcgaagcatt catcccctat ctttgcaagg actgcatttg aaatgtaatc agagaaggcc
      361 attgttatgt catattctac agatgtccca gtcattaggg ctgcaccccg ggcaacatcc
      421 acaacccttt caaagagatc cctcatctgg gtaacctttg gagccctcac ctcatactta
      481 acaagactcc tgtcctgaac aacatttgga gatactcctc cggcatcaac ataggcatag
      541 tggatcctgg cctggtcaat catgtgctcc ctaaggtaat tcacacctac actcataagc
      601 tctgcagcat ccagggcact tctacccagg tggggacttc cccctgcatg ggctgtggtt
      661 cccttgaacc tgaagttagc gcccattata gcaacagtcc ggtcagactg tacttcattc
      721 ttggtggcag ggtgccatgt gtagataaaa tcagcctcgt caaaaacccc atcccttgcc
      781 ataaactgct tggagccggc tccctcttca gcaggagtgc catagtagat aaccgttccc
      841 tccctgccgg ttctctttag aaattccttt agggcaattg cagctccaag agagcctgtt
      901 cccagaagat tgtgcccaca tccatgccct ggtccatcct tcacaagtgg ggacttttcg
      961 gtaattcccg ccctttgact gagggcatcc agagcatcgt actcacctag aaatcccgct
     1021 acaggctttc cggttccgaa tctaccgata aatgcagttg gcatatccga aacaccttca
     1081 accacatcaa aaccctcatc ccttaaaata ttcatcaata ttcttgcaga tttctcttcc
     1141 atgtagggaa gctctgctgc ctcccatatt tttctactgg catcaacaag tgttttctct
     1201 ttttcttcaa ttatttcttc aattagcctc aactcgtcca attccttacc tccctataga
     1261 atcttgctta aaaattcaat tgttctgggg ttctttggtc tgttgaagat atcatctggg
     1321 ctaccctgct cctgaatgat tccttcatcc ataaagagaa ccctatcgct tacttccctt
     1381 gcaaaaccca tttcatgggt tactatgaca attgtcattc ccgtttcaac cagactcttg
     1441 ataacatcca gcacctcctt gaccatctcg ggatcaaggg cactggtggg ctcgtcaaag
     1501 agcataacat ccggctccat ggccagtgcc cgaacgattg cgagcctttg cttctgaccc
     1561 ccagaaagcc ttgtgggata ctcatccttc ttgtctgcca atcccaccct cttaagcagg
     1621 tcatctgctt ttttttcagc ctcttttctg ggcaccttct tttccagtat cggtgcaagg
     1681 gttatatttt caaggacggt caaatgtgga aatacgttaa acagttgaaa aaccataccg
     1741 attttttgtc tgtgaaggtc aacattaacc tttttatcgg caaggtcgat ccccttgaag
     1801 atgacctttc ctcctgttgg tttttcaagt agattgaggc atctcaggaa ggtgctcttt
     1861 ccactgccgg aagggcctat aattgatatt acctcgccct cgtatatgct ttcatttatg
     1921 tccttcaaga ccttcagctc accaaaggac ttatccagat tttcaaccct gatcagttct
     1981 tccctactca tttgcctgca gcctcctttc aaatatgtcc agcccctttg acaggacagt
     2041 ggtcactaca aggtatatga tacccactat gaccaaggaa ggtattgcca gaaaggttgc
     2101 cgattgaaca gtcctgtagg aggtcatcag ctcctggaca aagaataccg aagccaggga
     2161 tgtctgcttc accatgttta tatactggtt gcctagggct ggcagtatgt tttttatggc
     2221 ctggggcagt atgatccttc tcattgtatt tctattgctc attcccaggc tcttggccgc
     2281 ctctgtctgt cccttgtcaa cggcctgaat cccagccctt attatctctg caacatatga
     2341 gctagcattg actatgaggg ctatgacaat gcaggcggtg tctgacagtg tgacagggga
     2401 tatcttcggc agaagaatcc a
//
LOCUS       DASRQF010000275         2190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_825401, whole genome shotgun sequence.
ACCESSION   DASRQF010000275 DASRQF010000000
VERSION     DASRQF010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825401"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1816
                     /gene="abc-f"
                     /locus_tag="VFD79_07335"
     CDS             191..1816
                     /gene="abc-f"
                     /locus_tag="VFD79_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HZH93900.1"
                     /translation="MLLIECKDIRKYYGERLILEIEELKIYEGDRIGIVGINGSGKTT
                     LLNILSKRLEPDQGYVRTYGSSSYVSQIDDPDINILSDEMASRFGVDFSWKESMSGGE
                     KTRFKVAAALEENSDVLFADEPTSNADLESIRLMEDRFRARNGTLVLISHDRSFLDRL
                     CNQILEIEDGKVSTYKGNYTAYKDQKIMELERKQFEYQEYVRERKRLQSAIGEIKGKS
                     SSIRQAPRRMGNSEARLHKMGGQRAKATLDSAAKNIEKRIEHMERKEKPREEPKIKFD
                     IPDSSRSHSRILVEGKNLNKSFDTRVIFKDSEFMIENGAKVALIGPNGCGKSTLIKMI
                     MDGEEGIRVAKSAKIGYFSQDLSILNEGRSIIENVMENSIYSETFARIFLARLLFRKE
                     AVHRKVKVLSGGERVKVSFAKILMEDFNMLILDEPTNYLDIMSLEIIEEALQEYNRTL
                     LFVSHDRKFIGSIADQVMTIEGGKIDLYKGNYEEYLAGKDKSLDKREEQIMVLRNRLS
                     ELISRISMPSKGDDLESLDIEYRNVLEELNRIR"
     gene            1921..>2190
                     /locus_tag="VFD79_07340"
     CDS             1921..>2190
                     /locus_tag="VFD79_07340"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HZH93901.1"
                     /translation="MYKLYCRLYQMILRGVSGFLPWRMPELLQGEGSIKRLPVFIKEK
                     GIESLLIVTDKGIRSIGLMDELLMDLEQVEIDYIVYDKTVANPTID"
BASE COUNT          732 a          335 c          572 g          551 t
ORIGIN      
        1 agaaccctaa tgaggttaat ttgatgtgtg caaatttaga ttaatgagtg gatagtattg
       61 tatttatcgt atgtagagat atttgtctgg gaattacctt tttaattaat tgcatttgta
      121 ctgcaaaagg ggtagttctg catatgatgt ttaaatgtac tatcctctcc agacacaggg
      181 aggatttttt atgttattga tagagtgcaa ggatatcagg aagtattatg gtgaaagatt
      241 gattcttgag attgaggagc tcaagattta tgaaggtgac cgaataggta ttgtgggcat
      301 aaacggttca gggaagacaa ccctgcttaa catactcagc aaaaggcttg agccagacca
      361 aggctatgtc agaacctatg gaagcagctc ttatgtttca cagattgatg accctgacat
      421 aaatattcta agtgatgaaa tggcgtccag atttggagtg gacttttctt ggaaggaatc
      481 aatgagtggc ggtgaaaaga caagattcaa ggttgcagct gccttggagg aaaattcaga
      541 tgtcctattt gcagatgaac ccacaagcaa tgcggacctg gagagcataa ggcttatgga
      601 ggaccgcttc agggccagga atggaacgct ggttttgatt tcccatgaca ggagtttttt
      661 ggataggttg tgtaatcaga ttctcgaaat agaggatggg aaggtaagca cctacaaggg
      721 gaactacacg gcctataagg accagaagat catggagctt gaaaggaaac aatttgaata
      781 ccaggaatat gtcagggaaa ggaaacgact tcaatcagca ataggtgaaa taaagggcaa
      841 gagtagctca ataagacagg cacccagaag gatgggaaat tctgaggcca ggctccataa
      901 aatgggaggt caaagggcaa aggcaaccct tgatagtgca gccaagaaca tcgagaagag
      961 gatcgaacac atggagagga aggagaagcc cagagaagag ccaaagatta aatttgatat
     1021 cccggattcc agtagaagtc acagcagaat tctcgtcgag ggtaaaaatc tgaacaaatc
     1081 atttgatacc agggtgattt tcaaggactc ggaattcatg atagagaatg gggctaaggt
     1141 tgcattgatt gggccaaatg gctgcggtaa aagcaccctg atcaagatga tcatggacgg
     1201 tgaagaaggt attcgtgttg caaaatcagc aaaaattgga tatttcagtc aggacttgag
     1261 tatattgaat gagggaaggt ccatcataga aaatgtaatg gagaacagca tatattctga
     1321 aacattcgcg agaattttcc ttgcaaggct tctattcaga aaagaggcag tccacaggaa
     1381 ggttaaggtc ttgagtggag gagaaagggt aaaggtttcc tttgcaaaga tccttatgga
     1441 ggactttaac atgttgattc ttgatgaacc taccaactac cttgatatca tgagcctgga
     1501 gataattgag gaagcccttc aggaatacaa cagaacattg ctctttgtat cacatgacag
     1561 aaaattcatc gggagcattg cagaccaggt aatgacaatt gagggaggga agattgacct
     1621 ctacaaggga aactatgagg aatatctggc tggtaaagac aaatccttgg ataaaaggga
     1681 ggaacagatt atggttctta ggaacaggct atctgagtta attagcagga tatccatgcc
     1741 atcaaagggt gatgacctgg agtctctgga tattgaatac agaaatgttc ttgaagaatt
     1801 aaatagaatt agataataat cgagtcccat taccaggaag taaacggaat gggactttat
     1861 tttttccaca gggagatgaa ggatcttatt tgggtaatta ttgggtgagg aggtgggaaa
     1921 atgtacaagc tatactgtag gctctaccag atgattttaa gaggggtatc cggattcctg
     1981 ccatggagga tgcctgaatt actgcaaggg gagggaagca tcaaaagatt gccagtattt
     2041 ataaaggaga aggggataga aagtcttcta atagtcaccg acaagggaat aagatcaatc
     2101 ggtcttatgg atgagttact tatggacctt gaacaggtgg aaatagatta catagtatat
     2161 gacaaaacag tggccaaccc gacaatagat
//
LOCUS       DASRQF010000276        12712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1406510, whole genome shotgun sequence.
ACCESSION   DASRQF010000276 DASRQF010000000
VERSION     DASRQF010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 12712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12712
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406510"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VFD79_07345"
     CDS             <1..574
                     /locus_tag="VFD79_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93902.1"
                     /translation="LEDTIQERNLYLSLSTAAEFISQKAYSAKGMYLAGIDEIEAEGS
                     LGYSLTFRYRVRGIPVLLGNREVQEYVQIEVFNNQVRSYRQLFRGEMDLDIASLQDSR
                     QIMSSFDVIDKNYLFLESEYLEHTGETREQLGDQVVQRVLSSVEDITISYYDPGLKDQ
                     EERLIGVWIIRAHGSVYAFNAYSGMLVYER"
     gene            586..1431
                     /gene="yycI"
                     /locus_tag="VFD79_07350"
     CDS             586..1431
                     /gene="yycI"
                     /locus_tag="VFD79_07350"
                     /inference="COORDINATES: protein motif:HMM:NF021186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HZH93903.1"
                     /translation="MDWSKAKTILIVALLFTNIFLAFFLFGDQESGNTVLDPEFLQQT
                     EDLLGRNGIILDAELPTDIQQLYNLTVEYETMLPGSLNERFFGSEGAINRSEGALVEI
                     ENGDETITIINEKLMIYENTGQEAGNSIESIDQATELALGFLQDRGYETDDMGLSYWR
                     ETDNGYYLDYTKVFQDSYIELSFTVFRIVDGRIARMERTWLNTKEIEETDYKIVPAPK
                     AILELLSMQDASNKTITDISLGYYFDPEKQDYLDDYQQAKEGRAVPTWRVQFQDGYDV
                     ILDLE"
     gene            1515..2309
                     /locus_tag="VFD79_07355"
     CDS             1515..2309
                     /locus_tag="VFD79_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZH93904.1"
                     /translation="MTVKVCSLSSGSSGNCQYIETENTKLIIDAGFSGKRIEQLLDEI
                     QVDPRDLDGILVTHEHIDHSKGVGVLSRRYDIPVYANENTWKGMESIIKNVKTHNTKV
                     FSTNRNIELKDVLIHPFNVSHDASEPVGYIIFNNKTKLSFVTDTGWINEGIRKKISNS
                     HFYFMESNHDVRMLMEGSYPMMLKKRIMSLKGHLSNDDAADVLSDVLRGENEIVLLAH
                     LSQDNNKPELAYSTVRNGLMNQGFDVDKDLRLNMTNRDGISAIYSL"
     gene            2325..3470
                     /locus_tag="VFD79_07360"
     CDS             2325..3470
                     /locus_tag="VFD79_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZH93905.1"
                     /translation="MNDFNYGSYYRPPKRRGIFSYILVALIAGMIGGLVTAYIAPTYL
                     YGKIIPVPEIYSEGQLSMMNTVTINPTEEINVVAAVATKAVSSVVGITTVSIERDFFW
                     SRPVEGVGSGVVVSSDGYILTNSHVIGDGNAREIQVLFESGDKAQGRVLWYDATLDLA
                     VVKVSATNLHAAELGDSDEIRVGELAVAIGNPLGLDFQRTVTSGVISGLHRSVQVNQY
                     NVMEDLIQTDASINPGNSGGPLLNSKGQVIGINTAKIQTGEGLGFSIPINMVKPVLEE
                     IVEEGTFNNVYIGFTGSEVEIYEVQMDTDLPAESGVVAIEILPGSPADQAGLRSLDVI
                     TYVDDEQVTSMANLRKILYNYRIGDSAQLTIDRGGEIIYVTVRFREF"
     gene            3480..4250
                     /gene="surE"
                     /locus_tag="VFD79_07365"
     CDS             3480..4250
                     /gene="surE"
                     /locus_tag="VFD79_07365"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HZH93906.1"
                     /translation="MKILLANDDGIMAEGIHTLAKALEKEHDVIIVAPETQRSAQSHA
                     ITLHKPIVIKEVELEGIKSPAYSISGTPADCVRVGMEELADDKIDIVFSGINMGLNSG
                     MDILYSGTVSAAIEGNLYGVPSVAVSAEWTPNGLNYEIAAKYALQVLEKMDTEKMTMP
                     MVLNLNTPFNLHDETKPIKVCKIGGPIYDYYLAEKNGNGERTLKVMGRKDADLKESTD
                     RYHLSEGFATLTPLSYDFTNFHLMEKVRQCMEEDSNCE"
     gene            4330..4845
                     /gene="tpx"
                     /locus_tag="VFD79_07370"
     CDS             4330..4845
                     /gene="tpx"
                     /locus_tag="VFD79_07370"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HZH93907.1"
                     /translation="MSRRTDAVTMGGNPVTLVGTEVKVGDKAPDFTALKADLSPYSLS
                     DVKDKVKIISVVPSLDTGVCELQTIRFNDEAAELEDVAILTISMDLPFAQKRFCSAND
                     IDKVVTLSDHRDLDFGTKYGFEIEELRLLARGIVVLDEDNIVRYVQYVPEATDHPDYD
                     KALEAAKSLIN"
     gene            4975..5454
                     /gene="rlmH"
                     /locus_tag="VFD79_07375"
     CDS             4975..5454
                     /gene="rlmH"
                     /locus_tag="VFD79_07375"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041250629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HZH93908.1"
                     /translation="MNIDILAVGKIKEKYLNEGIGEYSKRLSRYCSLNIIEVDDEKSP
                     ENLSDKEMEMIKAKEGEKLMSKIPSGAFVVALAIEGKQLSSEALSEKISDIMVDGNSH
                     IVFVIGGSLGLSGEILKRANLKLSFSKMTFPHQLMRMILLEQIYRSFRIMRNEPYHK"
     gene            5632..5940
                     /locus_tag="VFD79_07380"
     CDS             5632..5940
                     /locus_tag="VFD79_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZH93909.1"
                     /translation="MKRPNVDLSLKIAVVAPDGNFVSYCGMWYDIRSDFAVIEPVATD
                     PDHRRMGLGKAAVLEGIRRVGEIGARKVLVGSSQQFYYSIGMRPFSTASEWRRLKGNE
                     "
     gene            5933..6865
                     /locus_tag="VFD79_07385"
     CDS             5933..6865
                     /locus_tag="VFD79_07385"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HZH93910.1"
                     /translation="MSKKRVCVYGIGGVGGYLGGMLAVGAKDNYEVYFVARGNHLEEI
                     KKNGLALITPSKGEIRIHPTRAVENVGDLPEMDIIILAVKGYDLVEAVEDINKITKEN
                     TTIIAPLNGVDIHERIRSKLNRGIVLPSCIFVTSTVKEPGVVVHKGGTSLLVLGKDPQ
                     VEDYDSKELVEIINNAEIPHRWEEKPEIIIWEKFIMFAPFALVTAYSGKSFGQVMEDV
                     DLKGLIKNIMEEVVELARAKGIPVKDSLVETYLGNAQFYPYETRTSYQRDIEGGKGKD
                     EGDLIGGTIIRLGKEHGVPTPETENIYKKIGSKK"
     gene            7054..8490
                     /locus_tag="VFD79_07390"
     CDS             7054..8490
                     /locus_tag="VFD79_07390"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZH93911.1"
                     /translation="MSFLLFSSNNEIDKNTSVEEFTSHLNRQIYSLSDRYGIPGGAIA
                     IVRDGKVVWSDTFGYADIEEEKEITEATRFRVESISKSVTAWGVMKLVQDGMIDLDEP
                     ITKYIRKWSFPDSKFSYDSITIRHLLTHTSGMPLGDFMDRYPPGKKVPSLEESLGTKA
                     IPVDEPGKKFTYSNTGYNLLELLVEEVTGKDFDVYMKEKVLRPLGMENSTYNWEDIPE
                     GLVPTGYDIGGNPVESYVYPEKASGGMFATLEDVAAFMIAGIGETYVSGNDVLTPRSL
                     HQLYIAAVEKIGVYGLVFDGYGMGYYLEDLSNGQRSVANGGQGGGVMTYFHYVPETGE
                     GIVILTNSQRSWPFFGRIIDDWARWNGYEGAGFGKIPMAESVLWIFIEILAIVFLINH
                     YLIIRGLASGRSRFSMSGPLAAKRRIYKIVISVILLIGLLWAANQDYLFITAVFPIAS
                     GWFGVSAFLLAIGQLVSAFLFKDQPGEK"
     gene            8548..9516
                     /locus_tag="VFD79_07395"
     CDS             8548..9516
                     /locus_tag="VFD79_07395"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HZH93912.1"
                     /translation="MQNNYSFTDSGIVETNTIKDMNQWIRLTDPTEDETKEMMDRFGI
                     KPIYIKASLDIDENSRVEASNGQLFILVNMPYKDETGKFVSYNTLPLGIILTDKVLLT
                     VALRDNDVIQRILQTELQEITSRVTFVYQVLFRMVDLYLKYLKEIEKKSAVIEDYLNK
                     SLKNEELIKLLNLEHSLVYFSTALRSNQAVMEKIFSSHVSKVKNEKNLLTQKLLEYSE
                     ENEDKLKELIIENKQAIALIDIYTNILSNTMDAFASIISNNLNIVMKFLTIVTISLAM
                     PAIVTSFYGMNLSLPLQDRPFAFIGVILLSIVLMVVVTFTVNRKRF"
     gene            9539..9910
                     /locus_tag="VFD79_07400"
     CDS             9539..9910
                     /locus_tag="VFD79_07400"
                     /inference="COORDINATES: protein motif:HMM:NF021483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HZH93913.1"
                     /translation="MIEKGASMAEHKIYKMSFAKVYPMYVAKAEKKGRTKEEVDEIIL
                     WLTGYSQEDLEDQLEKETDFEGFFGQAPNMNPSRELIKGVVCGVRVEDVEEPLMQEIR
                     YLDKLVDELARGKKMEKILRK"
     gene            complement(9956..10990)
                     /locus_tag="VFD79_07405"
     CDS             complement(9956..10990)
                     /locus_tag="VFD79_07405"
                     /inference="COORDINATES: protein motif:HMM:NF023156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HZH93914.1"
                     /translation="MTLKKIIGLRTLKTAFGAGLGIFLAHLLNLDYGVNTAIVVILSL
                     QNTKRKSLNLAGVRILASIIALSLAKLVFTLFGFGPISFSIYLLLFIPIVVRLKLNEG
                     LVPSSVLVSHLLASRSVSLGSLGNEFAQMVIGASIALVLNLYIPGIESHLINDLAAIK
                     RVKYRILESMKAGLVGGYESTDRSQLLDEMSQRISLANERILSETGENFSRGLDYHLK
                     YLKMESDQLESLRFMSRSVNYLSKYYELSSMAADSTEKALLQLQSIRMDKEKINEIKG
                     SLEALAEPTQQSSLEEFQARATAFQYLEDLIGLLDIRTDFVASLSFSDRKLFREMHSK
                     IREMENVALD"
     gene            11455..12288
                     /locus_tag="VFD79_07410"
     CDS             11455..12288
                     /locus_tag="VFD79_07410"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZH93915.1"
                     /translation="MFKRMYEYMSRPKLYAKSTFIFWDDEHVSKGLLRAHLDPDVEAA
                     SRSGVFMDRSVEWIAEVAPAAEFNRVLDLGCGPGLYAERLTRKGYHVTGIDFSKRSID
                     YAKRQTEENGQDITYIYKDYMNIDYEDQFDLVILIFTDFGVLSDEDRGDLLRRIHKAL
                     RHGGRGIIDVSSHKEFEDRMENSSWHISEGGFWKPERHICLEGHLIYEDNIRLDQYVI
                     IEKEGKVETVRNWFKPFTREEIANEVRMAGFDRVKVYSDVAGKAYSDDSKTICLLMEK
                     K"
     gene            12528..>12712
                     /locus_tag="VFD79_07415"
     CDS             12528..>12712
                     /locus_tag="VFD79_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93916.1"
                     /translation="MGEDKVLNHTLEVLEKEGVKSAYDYVSRHKKDIPKPSGQYYNFL
                     YCLAALDGKSDEAMGYL"
BASE COUNT         3980 a         2300 c         3211 g         3221 t
ORIGIN      
        1 attggaggat acaatccagg agagaaatct ctacctgagc ctttcaacgg cagcggaatt
       61 catatcgcag aaggcttatt cagccaaggg catgtacctt gctggaatag atgagattga
      121 agccgagggt agtctaggat actcactgac cttcaggtac agggttaggg gaatacctgt
      181 gcttcttggt aacagggagg ttcaggaata cgttcagatt gaggtattca acaatcaggt
      241 aagaagctac aggcagcttt tcagagggga gatggacctt gatattgcaa gtcttcagga
      301 cagcaggcaa atcatgtctt ccttcgatgt aattgacaag aattatcttt ttctggaaag
      361 tgaatacctg gagcacacgg gagaaactag ggagcagttg ggagaccagg tggtccaaag
      421 ggtcctttca tcggttgagg acataacaat ctcctactac gatccagggc ttaaggatca
      481 ggaggaaaga cttatcggag tatggataat aagggcccat ggcagtgtat atgcctttaa
      541 cgcatattcg ggcatgctgg tttatgaaag ataggagaat gacggatgga ttggtccaaa
      601 gctaaaacaa tacttatagt tgcacttctc tttaccaaca tatttctggc attctttctc
      661 tttggagacc aggagtctgg aaatacagtt cttgaccctg agtttctcca gcaaacagaa
      721 gaccttctgg gaagaaacgg tataatcctc gatgctgagc tgccaactga tattcagcag
      781 ctctacaacc taaccgtgga gtatgaaacc atgcttccgg ggagcctcaa tgaaaggttt
      841 ttcggatctg agggagccat caataggagc gagggcgctc tagttgaaat tgaaaacgga
      901 gatgaaacaa taaccataat taacgaaaag ctgatgatat atgaaaatac aggccaagag
      961 gccggcaatt ccatagagag cattgaccag gccactgagc ttgcacttgg cttcctccag
     1021 gatagaggtt atgaaacaga tgatatggga ctttcctact ggcgggaaac agataatgga
     1081 tactatcttg actataccaa ggtattccag gattcataca tcgagcttag ctttaccgtg
     1141 tttagaatag tggatggaag aattgcaaga atggaaagga cctggctcaa caccaaggaa
     1201 atagaggaga ccgactacaa gatagtaccc gctccaaagg caatcctgga gcttttaagc
     1261 atgcaggatg ccagcaacaa gacaataaca gacatctccc ttggctacta tttcgaccct
     1321 gaaaagcagg attatcttga cgactaccag caggccaagg aaggtagggc cgtgccaacc
     1381 tggagggttc aatttcagga tgggtacgat gtgatactcg atcttgaata gaagaacgaa
     1441 attaagaagg gtaagccttc tttttttttg catgttacta tataattaaa gaagcaaagg
     1501 aggaggtttt ccaaatgact gtcaaggttt gctccctttc aagtggaagc agcggaaact
     1561 gccaatatat tgaaacagag aacacaaaac ttattataga tgcaggcttt tcaggaaaga
     1621 gaatagagca gctgctggat gaaatccagg tggacccaag ggatctggat ggcatactgg
     1681 tgacccatga gcacattgac cattcaaagg gggtaggggt cctttcaagg cgctatgaca
     1741 ttcccgttta tgccaatgag aatacctgga agggaatgga gtcaataata aagaatgtaa
     1801 agacccacaa cacaaaggtc ttttcaacaa acaggaacat tgagcttaag gatgtcctga
     1861 tccacccatt caatgtttcc cacgacgcca gtgaacctgt tggctatata atattcaaca
     1921 ataagacaaa gttgagcttt gttacagata cagggtggat aaacgagggt ataaggaaaa
     1981 agataagcaa ttcccatttc tactttatgg aatcaaacca tgatgtgagg atgcttatgg
     2041 agggaagcta ccccatgatg ctcaagaaaa ggatcatgag ccttaagggg cacctttcca
     2101 atgacgatgc agcggatgtc ttgtcagatg ttttgcgggg agagaacgaa atagttctac
     2161 ttgcacactt aagccaggac aacaacaagc cggaacttgc ctattcaacc gtaagaaacg
     2221 ggctgatgaa tcaagggttt gacgtggaca aggaccttag actcaacatg accaacagag
     2281 atggaataag tgcaatatat agcctttaat aaggaggaaa agggatgaat gacttcaatt
     2341 acggaagcta ctacaggccg cccaaaagga gaggcatctt ttcatatata ctggtagctc
     2401 ttattgctgg aatgattgga ggccttgtaa cagcatatat agcacccaca tacctgtacg
     2461 gaaagataat tcccgtaccg gaaatatact ctgaaggcca gctatccatg atgaataccg
     2521 tcacgattaa tcccacagag gagataaatg tggttgccgc ggttgccacc aaggcggtta
     2581 gctcagttgt tggcataact acagtatcaa ttgaaaggga tttcttctgg tccagacctg
     2641 ttgaaggtgt tggttcggga gttgtagtaa gcagcgatgg gtatatcctg accaactccc
     2701 atgtaattgg agatgggaat gcccgggaga tccaggttct ctttgagagc ggagataagg
     2761 cacagggtag ggttttgtgg tacgatgcta cactggatct tgcagttgtg aaggtaagcg
     2821 ccacaaatct tcatgcagca gagcttggtg actcagatga aataagggtt ggagagctgg
     2881 ctgtggctat cggcaaccca ttgggacttg attttcaaag gaccgtaacc tctggggtaa
     2941 ttagcggtct ccacaggtct gtccaggtaa accaatacaa tgtaatggag gacctgatac
     3001 aaacggatgc atcaataaac ccaggaaaca gtggaggacc cctgcttaat tccaaaggtc
     3061 aggttatcgg catcaataca gcaaagatac agacaggaga gggactgggg ttttcaatac
     3121 caataaacat ggtaaagcct gtcctggagg agatagttga ggagggaacc ttcaataatg
     3181 tatatatagg atttacaggt agtgaggttg aaatatacga ggttcaaatg gatacggacc
     3241 tacctgctga gtccggagtt gtggcaattg agatacttcc aggttcacct gcggaccagg
     3301 cgggattaag gtctcttgat gtaataacat atgtggatga tgaacaggtt acaagtatgg
     3361 caaacctgag aaagatacta tacaactata ggatcgggga cagcgcccag cttacaatag
     3421 acagaggcgg ggagataata tatgttacgg ttagatttag agagttctag gaggaagaga
     3481 tgaagatatt attggcaaat gatgacggaa ttatggccga ggggatacac accctggcaa
     3541 aggctctgga aaaggagcat gatgtaataa ttgtagcacc agaaacccaa cgaagtgccc
     3601 agagtcacgc aatcacactc cataagccca tagttatcaa ggaagtggag cttgagggaa
     3661 taaaatcacc tgcctacagc atatcaggta caccggctga ttgcgtcagg gttggaatgg
     3721 aggagcttgc agatgacaag atagacatag tcttttcagg tataaacatg ggccttaact
     3781 caggtatgga tatcctctac tcaggaaccg tgtcagctgc aatagaagga aacctctacg
     3841 gagttcccag tgtagccgtg tcggcagagt ggactcccaa tgggctcaac tatgaaattg
     3901 ctgctaagta cgcgcttcag gttttggaaa agatggacac tgaaaaaatg actatgccca
     3961 tggttctgaa tctgaatact ccattcaatc ttcacgacga gaccaagcca atcaaggtct
     4021 gcaagattgg aggtccaata tatgactatt acctggccga aaagaatggc aacggtgaaa
     4081 ggacattgaa ggtgatgggc aggaaggatg ccgaccttaa ggagagcact gacaggtatc
     4141 atctaagcga gggctttgca acattgaccc ccctaagcta tgactttaca aatttccacc
     4201 tgatggagaa ggtaaggcag tgcatggagg aagattcaaa ttgtgaataa tggacaggtt
     4261 tacgggtatc tatgcaatac gtttccaaaa gggagctaaa attaggattt tgtaaaggag
     4321 atagatacaa tgagtagaag aacagatgcg gtaacaatgg gtggaaatcc tgtaactcta
     4381 gttggtacag aggttaaggt tggagacaag gcacctgact tcacggccct aaaggctgac
     4441 ctgagtcctt acagcctgag tgatgttaaa gacaaggtaa agatcatatc ggttgtccct
     4501 tccctggata cgggcgtgtg tgagcttcag accataagat tcaacgatga ggcagctgag
     4561 cttgaggatg tggcaatcct aaccatatcc atggaccttc catttgccca gaaaagattc
     4621 tgcagtgcaa atgacataga caaggtagtt acactatctg accataggga cctggatttt
     4681 ggaaccaaat acggatttga gattgaggaa ttgagacttc ttgcaagggg aatcgttgtt
     4741 ttggatgagg ataatatcgt aagatacgtt cagtatgtac cagaggcaac agatcaccca
     4801 gactacgaca aggctcttga agcagccaaa agccttataa attaggatta aaattggaaa
     4861 ctggaatagc cttccctcgg gagggcttgt ttttttgtca aaatatactc catatatgct
     4921 attattaaag taagagaaag ctggaatggc gaaaacaatg cactggagga agaaatgaac
     4981 atagatatct tagcagtggg gaaaattaag gaaaaatacc tgaatgaggg aataggcgaa
     5041 tactccaaga ggctctcaag gtactgcagc ctcaacataa ttgaagttga tgatgaaaag
     5101 tcgccagaga acctttcaga caaggaaatg gaaatgatca aggcaaaaga gggagaaaag
     5161 cttatgtcaa agattccttc tggggctttt gtcgtagccc tggcaataga gggaaagcag
     5221 ctgtcatcag aagcgctttc tgaaaaaatc agcgacataa tggttgatgg aaattcccat
     5281 attgtgtttg taatcggtgg atccctcggc ttgtccggtg agatcctgaa aagggccaac
     5341 ctgaagctgt ccttttcaaa gatgaccttc ccccaccagc ttatgaggat gatccttctt
     5401 gagcagattt acagatcctt cagaataatg aggaacgaac cctaccataa gtgagtcacc
     5461 ggggacggtt atttttgact cgttttgtaa tggatacaaa gacgttgtta ggaggaaacc
     5521 tatgacctat accagtacta tcgtgttctc tggaagggtt ttaaccatga gctgaatggt
     5581 gagggcgagc tgaaattctc cgaagaaaat gtcaaggcag ggaaccttca gatgaagcgt
     5641 ccaaatgttg acctgagcct taaaattgca gtagtggcac ctgatggaaa ctttgtctcc
     5701 tattgcggta tgtggtatga cataaggagt gattttgcgg taatagagcc ggttgcaaca
     5761 gatcctgacc atagaaggat gggattgggc aaggcagctg tacttgaggg aatcagaagg
     5821 gtcggagaga ttggagccag gaaagttctg gtaggctcat cccagcaatt ctactacagt
     5881 atcggtatga gaccattttc aaccgccagt gaatggagga ggttaaaggg aaatgagtaa
     5941 aaaaagagta tgcgtatatg gcataggagg tgtgggaggt taccttggcg gtatgctggc
     6001 agtcggagcc aaggataact atgaggtcta ttttgtagcc agggggaatc atcttgagga
     6061 aataaagaag aatggactcg cccttatcac accatcaaag ggagaaatcc gaatccatcc
     6121 cacaagggct gtagaaaatg taggtgacct acctgaaatg gacattataa tccttgcagt
     6181 aaagggatat gaccttgttg aggcagttga agacattaac aagataacca aagaaaatac
     6241 aacaataatt gcacccctta atggtgtgga catccacgag agaataagat ccaagcttaa
     6301 cagggggatt gtcctacctt catgtatctt tgtcacttca acagtgaagg agccaggggt
     6361 ggtggtccac aagggtggaa caagcctgct ggtccttggt aaagatccac aggttgagga
     6421 ttatgattcc aaggagcttg tggaaataat aaataatgca gaaatccccc ataggtggga
     6481 agaaaaacca gagattataa tctgggagaa attcatcatg tttgcgccat ttgccctggt
     6541 gacagcctac tcagggaaat ccttcggcca agttatggag gatgtggacc ttaagggtct
     6601 tatcaaaaac ataatggagg aggttgtgga gcttgcaagg gccaaaggga ttccagtaaa
     6661 ggattcactg gtggaaacct accttggaaa tgcccagttc tatccctatg aaaccagaac
     6721 ctcctaccaa agggatattg aaggtgggaa aggcaaggat gagggggacc ttatcggagg
     6781 aacaataata agacttggta aggagcatgg ggtgccaact ccagagactg aaaacatata
     6841 caaaaagata ggttcaaaaa aataggactg gttgcttgaa aaaaattcga gaaaattttc
     6901 aaaagcaccc ttgactctcc tgttggggta gacaatagaa tagaatggaa gagtggtgcc
     6961 taccatatga aagggaatgg accagctatt agaaaattaa taagaagtaa agctcaaggg
     7021 acattgattg tcttacttgt aagtattctt acaatgagtt ttctattgtt ttcttcaaac
     7081 aatgaaatag ataaaaacac atcagtggaa gaatttacaa gccaccttaa tcgacaaatc
     7141 tatagtctaa gtgatagata tggaattcct ggcggtgcaa ttgcaatagt aagggatggt
     7201 aaggtcgtct ggtctgatac atttggatat gccgatattg aagaggaaaa ggagattaca
     7261 gaggctaccc gcttcagggt ggagtcaatt tctaaatcag taacagcctg gggggtcatg
     7321 aagctggtcc aggatggcat gattgacctc gatgaaccca taacaaagta catcaggaag
     7381 tggagctttc cagattcaaa attctcttat gacagtataa ccatcagaca tctgctgacc
     7441 catacttcag ggatgccctt gggagatttt atggataggt acccaccagg aaagaaagtc
     7501 ccctcgctgg aggaaagtct tggaacaaaa gcaattcccg tggatgaacc aggtaaaaag
     7561 ttcacctatt caaacacagg ctacaacctc cttgagctat tggttgagga ggtaacagga
     7621 aaggatttcg atgtatacat gaaggaaaag gtattgagac ctttagggat ggaaaattca
     7681 acctataact gggaggatat ccctgaagga ctggtcccaa caggctatga tattggaggg
     7741 aatccggttg aaagctacgt ttatccggag aaggcatctg gtggtatgtt tgcaactctt
     7801 gaagatgtgg ctgcattcat gattgcgggc ataggggaga catacgtcag tggcaatgat
     7861 gttctgactc ccaggagctt acaccagctc tacatagcag ctgtggagaa aatcggagta
     7921 tatggcctgg tatttgacgg atatggaatg ggatattatc tggaagacct gtcaaatggt
     7981 caaaggtcgg ttgctaacgg cggacagggt ggaggggtga tgacctactt tcactatgtg
     8041 ccggaaactg gtgaggggat agtgattctg accaacagcc agagaagctg gcctttcttt
     8101 gggaggataa ttgatgactg ggcaaggtgg aatggttatg aaggtgctgg atttggaaag
     8161 atccctatgg ctgaaagtgt gctgtggatt tttatagaaa tccttgcaat agtgtttttg
     8221 attaatcatt atctcataat aagaggactg gcatcaggaa ggagtagatt ttccatgtca
     8281 ggtccattgg cagcaaaaag acggatttat aagatcgtaa tatcagttat cctactgata
     8341 ggactcttat gggctgccaa tcaggattat ctgtttataa ccgctgtctt tcccattgca
     8401 tccggttggt ttggggtttc tgcatttctg ctggcgattg ggcaattagt atcagcattc
     8461 ctgttcaagg atcagcctgg tgagaaatag tagggtttag gggtataaag tagctgaggt
     8521 ataagatgca gtagcgggag tgatttcatg caaaacaatt attcgttcac agacagtggg
     8581 atagtggaaa ccaacaccat aaaagacatg aatcaatgga ttcgcctaac agatccaacg
     8641 gaggacgaga ccaaggaaat gatggacagg tttggaataa aaccaatata cattaaggct
     8701 tcgttggata tagatgagaa ctcgagggtt gaagcaagta acggacagct ttttattctg
     8761 gttaatatgc cttataagga cgagactggt aagtttgtat cctacaatac cttgcctctg
     8821 ggtataattc tcacggacaa ggtcttgctt actgtggcct taagggataa tgatgtaatc
     8881 cagaggattc ttcagactga actccaggaa ataacaagtc gggtgacttt tgtataccag
     8941 gtacttttca gaatggttga cttgtatcta aaatacctta aggaaatcga aaagaagtcc
     9001 gctgtcatcg aggattacct taacaaatcc cttaaaaatg aagagttgat taagcttttg
     9061 aacctggaac acagccttgt gtatttttca acagcattaa ggtccaatca ggctgtcatg
     9121 gagaaaattt tctcatcgca tgtaagcaag gtaaaaaatg aaaagaacct tttgacccaa
     9181 aagctgcttg aatacagtga ggagaacgaa gacaagctaa aggaattgat tattgagaac
     9241 aagcaggcaa tagcgctaat tgacatttat acaaacatac tcagtaatac aatggatgca
     9301 tttgcatcaa taatatcaaa caacctcaac atagtaatga aattcctcac aatagtgacc
     9361 atatcattgg caatgccggc aatagtaact agcttctatg gcatgaacct cagtcttccg
     9421 cttcaggata gaccttttgc tttcataggg gtgattctgt tgtccattgt attaatggtg
     9481 gttgttacct ttacagtgaa tagaaagaga ttttgaaatt ttatgcaaga attagtgaat
     9541 gattgagaaa ggagctagca tggctgaaca caagatttat aagatgagct ttgcaaaggt
     9601 ctacccaatg tatgtggcaa aggccgaaaa gaagggtcgt acaaaggaag aagtggacga
     9661 aatcatcctt tggcttacgg gctacagtca ggaagacctg gaagaccagc ttgagaagga
     9721 aactgacttt gaaggcttct ttggtcaagc acccaatatg aacccttcga gggaactgat
     9781 caagggagtg gtctgtggag taagggtgga ggatgtagaa gagcccctga tgcaggagat
     9841 cagatatctg gacaagcttg tagatgagct tgcaaggggc aagaagatgg agaagatttt
     9901 aagaaaatag gagaaggtac ctgatggaat aatccaaaag gtacctttat ttttgtcaat
     9961 ccagagcgac attctccatc tcccgaattt tcgaatgcat ctccctgaaa agcttcctgt
    10021 cggagaagga caatgaagct acaaaatcag ttctgatatc aagtaggcct ataaggtcct
    10081 caagatattg gaatgctgtt gcacgagcct ggaattcctc caaggaggat tgttgggttg
    10141 gctcggccaa ggcttccaaa ctgcccttga tctcattgat tttctccttg tccatcctga
    10201 ttgattgaag ctggaggaga gccttttctg tactatcagc tgccatactg gaaagctcgt
    10261 agtatttact caagtagttt accgaacgac tcataaatct caaagactca agctggtcag
    10321 actccatttt cagatacttc agatgatagt ccaagccgcg gctgaagttc tctccggttt
    10381 cactaagaat cctttcattt gccagagaga tcctttgtga catttcatcc agcagctggg
    10441 atcggtcagt tgattcataa cctcccacaa gacctgcttt catgctttca agtattctgt
    10501 actttaccct ctttatggca gcaaggtcat taataagatg ggactcaata ccaggtatat
    10561 ataggttaag tacaagtgca atggaagccc cgattaccat ctgggcaaat tcattaccga
    10621 gacttccaag acttacgctt ctggaagcaa gaagatggct aacaaggacg gagcttggga
    10681 ccagaccttc attaagcttg agcctaacca caatggggat gaacaggagc agatagattg
    10741 aaaatgatat tggaccaaag ccaaacagag taaacacaag cttggccaac gatagtgcaa
    10801 ttattgatgc caatatcctt actccagcca ggttcaagga ctttctcttt gtattctgga
    10861 ggctaagaat aactacaatg gctgtattga ctccataatc caggttcagc agatgggcta
    10921 ggaatatgcc aaggcctgct ccaaaggctg ttttaagtgt cctaagacct atgatttttt
    10981 tcaatgtcaa caatcgatac atctccttac agactgtata tccagtcaca tacttgaaca
    11041 atacaatgat tctaacagtt tgatggggta cttgcaagat atattaattg gggaatatct
    11101 acgagatatc ctatccagca aaccccttta cactgatctg atgatgttat atcatatagg
    11161 tgtaataata tgatttgaag ggtggaatca agtccggaac acttgatgtt agagatcaaa
    11221 cagcataagt gaagcgcgga gatgaaagat aaaatcttaa attgaatcta cagtcaggaa
    11281 aaccgttatt tatgtaacgt gttttgtcgt gtctgttttc aatttggatt ttagtcttct
    11341 gtctgccggt aagtttcaac ctgttacagg gagccgtgga tgaaagtcca tggctctttt
    11401 tgcattcaat ccacgaatca tattattgca aaaaattaaa aggggagtgg ttttgtgttt
    11461 aagagaatgt atgagtatat gagtaggcca aagttatatg caaaaagcac ttttatattc
    11521 tgggatgatg agcatgtatc aaagggtttg ctaagagccc atttggatcc tgatgttgag
    11581 gctgcaagca gaagtggtgt ttttatggac aggtcagttg aatggattgc tgaggtggct
    11641 ccagctgcag agttcaatag ggtcttggac ctggggtgtg gtcctggact ttatgctgaa
    11701 aggcttacaa ggaagggcta tcatgtcaca gggatcgatt tttcaaaaag gtcaattgat
    11761 tacgcaaaga ggcagactga ggagaatggg caggatataa cgtacatcta caaggattat
    11821 atgaacattg actatgagga ccaatttgat cttgtgatat tgatattcac tgattttggg
    11881 gttttgtccg atgaagatag gggtgatctt ctcaggagga tccataaagc tttgaggcat
    11941 ggaggaagag ggataattga cgtttcaagc cacaaggagt ttgaggatag gatggagaac
    12001 agctcatggc atataagtga aggaggcttc tggaaaccag agagacatat ctgcctcgaa
    12061 ggacatctga tctatgagga caatattcgc ttggaccagt atgtgattat tgagaaggaa
    12121 gggaaggttg agactgttag gaactggttt aagcctttta caagggagga aattgcaaat
    12181 gaggtaagaa tggctggatt tgatagggtc aaggtctatt ctgatgtagc cggtaaagca
    12241 tattctgatg attcaaaaac catttgcttg ctcatggaga aaaaataatc aatgttaagc
    12301 ttgtgtccgt ggggagggac cccgtggaca cattctgctt caggtgatta agatagaaaa
    12361 tagcaaaaac actaagaaat gtgaattaat gagcatttga ggaaaatttt ctggtatact
    12421 tatgttgaaa agaagtggaa taatctgata gcgcaaagaa gtggcaagga tgtgccaagc
    12481 aagtaggccg gtttttgtct agagtaatga taatctaagg agggtgcatg ggagaggata
    12541 aggtcttgaa tcacacgttg gaagttttgg aaaaagaggg tgtaaagtct gcttatgatt
    12601 atgtatcaag gcacaagaag gacatcccca agccaagcgg acagtactac aattttctct
    12661 attgcctggc agcactggat ggaaagtcag atgaggcaat gggttacctg ga
//
LOCUS       DASRQF010000277         6627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_78246, whole genome shotgun sequence.
ACCESSION   DASRQF010000277 DASRQF010000000
VERSION     DASRQF010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6627
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78246"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3109
                     /gene="rpoC"
                     /locus_tag="VFD79_07420"
     CDS             <1..3109
                     /gene="rpoC"
                     /locus_tag="VFD79_07420"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HZH93917.1"
                     /translation="PMYEKQLLTETEYRDAVDKYHNSFRAMMGAEAVRSLLEKIDLES
                     EAKELRAQLKDATGQKKVRITRRLEVVEAFRNSGNRPEWMILDVIPVIPPDLRPMVQL
                     DGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPDIIVRNEKRMLQEAVDALIDNGRRG
                     RPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKFYQCGLPKK
                     MALELFKPFVMKKLVSDGHAHNIKSAKRMVERIKPQVWDVLETVIKDHPVLLNRAPTL
                     HRLGIQAFEPVLVEGKAIKLHPLVCTAYNADFDGDQMAVHVPLSSEAQAEARLLMLST
                     NNILAPKDGKPITTPSQDMVLGAYYLTVYREGEHGEGKIFKDYDELIHAYANGDVGMH
                     AKVKVRMKLDRNDRGQLVESTVGRFIFNENLPQDLGFVDRTVDKYGLEIDRVVDKKQL
                     GVIIDKCFRKHGNSVTAEILDHIKTMGFKYSTIGAISISMGDVVIPEEKHEMIKKAEV
                     VVDRFEKSYRRGLISDEERYERVIETWNQTTEDVTEILMDGLDPMNNIYVMAISGARG
                     SKNQIRQLAGMRGLMASASGRTVEVPIKSNFREGLSVLEFFISTHGSRKGLADTALRT
                     ADSGYLTRRLVDVSQDVIVREDDCGTDHYFLVKAYKDGKEILEELKDRIDGRYAFEDI
                     IHPKTGEIIVKTNEMITEEQAAFIQEIGIEEVKVRSVLGCKAKFGVCAHCYGRNLATG
                     KEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTGGVAAAVDITQGLPRVEELFEARKPKG
                     LAVISEIDGKVSINDNKKRKEVVVTNEDGEEARYSIVFGSRLKVREGDVIEAGDELTE
                     GSVNPHDILKIKGIKGVQNYIVKEVQRVYRLQGVDINDKHIEIIVRQMLSKVKIEEAG
                     DTDLLPGSLVNSMEFEKTNKKVLEEGGEPAVGRMTLLGITKASLATDSFLSAASFQET
                     TRVLTEAAIKGKEDFLLGLKENVIIGKLIPAGTGMRRYKNVEVVDDEIVPQEDENLVD
                     TDILQ"
     gene            3212..3460
                     /locus_tag="VFD79_07425"
     CDS             3212..3460
                     /locus_tag="VFD79_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HZH93918.1"
                     /translation="MFTDLKEQDKVVGAKQVKRSLSRSEVQTVFIARDAEKKVTGEIE
                     DLCKSKDVKVMYVDTMEELGEACGIDINAAVAALLNKN"
     gene            3475..3894
                     /gene="rpsL"
                     /locus_tag="VFD79_07430"
     CDS             3475..3894
                     /gene="rpsL"
                     /locus_tag="VFD79_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HZH93919.1"
                     /translation="MPTINQLVRKGREKVVYKSKSPALGMNLNTLKKKETESNSPQKR
                     GVCVAVRTITPKKPNSALRKVARVRLTNGMEVAAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIVRGTLDTAGVENRLQSRSKYGAKKPKVKK"
     gene            4010..4480
                     /gene="rpsG"
                     /locus_tag="VFD79_07435"
     CDS             4010..4480
                     /gene="rpsG"
                     /locus_tag="VFD79_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HZH93920.1"
                     /translation="MPRKGHIPKREIMPDPIYNDVVVTKLINSIMLDGKKGIAQNIVY
                     GAFDQIAEKTGEDPLEVFYKAMNNIMPVLEVKARRIGGANYQVPIEVRTDRRETLGLR
                     WLVLYSRKRGEKTMVEKLAKELMDAANGLGASVKKREETHKMAEANKAFAHYRF"
     gene            4539..6608
                     /gene="fusA"
                     /locus_tag="VFD79_07440"
     CDS             4539..6608
                     /gene="fusA"
                     /locus_tag="VFD79_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZH93921.1"
                     /translation="MPRQVSLEKTRNIGIMAHIDAGKTTTTERILFYTGKIHKIGETH
                     EGGAQMDWMEQEQERGITITSAATTAFWKDHRVNVIDTPGHVDFTVEVERSLRVLDGA
                     VALFCAKGGVEPQSETVWRQADKYRVPRLAFVNKMDIMGADFFRAVGMMKDRLKANAV
                     PIQLPIGKEENFTGIVDLIDMHARIYKDDLGQEIEITEIPADLIDLANEYRELLIESV
                     SESDEELMMKYLEGEELTSEEIMRGIRKATLDVKMTPVLCGSSYKNKGVQILLDAILA
                     YLPSPLDVPAIQGILPGTEEVVERHADDSEPFSALAFKIVTDPYVGKLAYFRVYSGSA
                     KAGSYVYNSTKGKRERIGRILQMHANKREEIDEVFAGDIAAAVGFKDTSTGDTLCLDT
                     DPVILEKMEFPEPVIHVAIEPKTKASQEKMSVALAKLAEEDPTFRTYTDEETGQTIIA
                     GMGELHLEIIVDRLLREFKVEANVGNPQVAYKESIQKPADAEGKYVKQSGGRGQYGHV
                     KLKVEPQEPGKGYEFVNAIVGGAIPKEYVNSVNNGIQEALESGILGGYPVLDIKVTLY
                     DGSYHDVDSSEMAYKIAGSMGVREALRKAGPTLLEPMMKVEVTVPEEYMGDVIGDINS
                     RRGRMEGMELVSGAQIIRAYVPLAEMFGYATDLRSNTQGRGLYTMQFDHYEPVPNSIS
                     EQVLGGK"
BASE COUNT         2197 a         1192 c         1721 g         1517 t
ORIGIN      
        1 ccccatgtat gaaaaacagc tccttactga gaccgaatac agggatgcag tggacaagta
       61 ccataacagc ttcagggcca tgatgggtgc cgaagctgta aggtctctgc ttgagaagat
      121 cgacttggaa tcggaggcaa aggaactgag ggctcagctt aaggatgcaa ccgggcagaa
      181 gaaggtaagg ataacaagaa ggcttgaggt tgtggaagca ttcaggaact caggcaatag
      241 acctgagtgg atgatccttg atgtgatccc tgtgattcca ccggatttga gaccaatggt
      301 ccagctggat ggaggaagat ttgcaacctc agaccttaat gacctttaca gaagagttat
      361 aaatagaaac aacagattaa agaggctact tgacctggga gcaccggata tcattgtaag
      421 aaatgaaaaa aggatgcttc aggaggcagt ggatgctcta atagacaacg gtagaagggg
      481 aaggcctgta acaggtcctg gaaacagacc actaaaatcc ctgtcagaca tgctaaaggg
      541 taagcagggt cgtttcagac agaaccttct aggtaaaagg gttgactact cgggacgttc
      601 agttatcgta gttggtccag agcttaagtt ctatcagtgt ggtcttccca agaagatggc
      661 attggagctg tttaagccat tcgttatgaa aaagcttgtt tcggacggac acgctcacaa
      721 catcaagagt gcaaagagaa tggttgaaag gatcaagcca caggtttggg acgttctaga
      781 aactgtgatc aaggaccacc cagtccttct aaaccgtgca ccaacccttc acagacttgg
      841 aatccaggct tttgaacctg ttcttgttga gggcaaggca atcaagcttc acccattggt
      901 ttgtactgca tacaatgctg actttgacgg tgaccagatg gcggtacacg tacccctatc
      961 atcagaggct caggccgagg caagacttct tatgctatca acaaacaata tccttgcacc
     1021 caaggatgga aagccaataa ccactccttc acaggacatg gtgcttgggg cctactacct
     1081 gacagtatac agggaagggg agcatggaga aggcaagatc ttcaaggatt acgatgagct
     1141 gatccacgca tatgcaaacg gggatgttgg aatgcacgcc aaggtcaagg tcaggatgaa
     1201 gcttgaccga aatgaccgcg gccagcttgt tgaaagtact gtaggtagat tcatattcaa
     1261 cgagaacctg ccacaggatc ttggatttgt tgacagaaca gttgacaagt acggcctgga
     1321 gattgacagg gtcgttgaca agaagcagct tggggtaata attgacaagt gcttcaggaa
     1381 acacggcaac tcagtgacag ctgaaattct tgaccacata aagaccatgg gctttaagta
     1441 ttctacaatt ggtgcaattt caatctccat gggtgacgtt gtcatcccgg aagagaagca
     1501 tgaaatgatc aaaaaggcag aggttgtagt agacagattc gagaaatcct acagaagagg
     1561 acttatctca gacgaggaaa gatatgagag ggtaattgag acctggaacc agacaactga
     1621 ggatgtaact gaaatcctga tggatggtct tgaccctatg aacaacatat atgtaatggc
     1681 aatatccgga gccagaggta gtaaaaacca gatcagacag cttgctggta tgcgtggtct
     1741 aatggccagt gcatcaggta ggaccgttga ggttccaatc aagtccaact tccgtgaggg
     1801 actgtcagtt cttgagttct ttatctccac gcacggttcc agaaagggac ttgcagatac
     1861 ggctcttaga acagcagact cgggatatct tacaagaaga cttgtagatg tcagccagga
     1921 tgtaatagta agggaggacg attgcggaac cgaccactat ttccttgtga aggcatataa
     1981 ggacggcaag gagattctgg aggagctgaa ggacagaatt gatggaagat atgccttcga
     2041 ggatatcatt catccgaaaa ctggagaaat catcgtcaag acaaatgaga tgatcacaga
     2101 ggaacaggca gcctttatcc aggaaattgg aatagaggag gtcaaggtta ggtcagtact
     2161 tggctgtaag gctaaatttg gagtatgtgc tcactgttac ggtagaaacc ttgcaacagg
     2221 caaggaagtc aacatcggtg aagcagttgg aatcatagca gcacagtcaa tcggtgagcc
     2281 tggaacacag cttacaatga gaaccttcca cacgggagga gttgcagctg ctgttgacat
     2341 cacccagggt cttccaaggg ttgaggagct tttcgaggct agaaaaccaa agggacttgc
     2401 agtgatatca gagattgacg gtaaagtaag cataaatgac aacaagaaga gaaaagaagt
     2461 tgtcgtaaca aacgaggatg gagaagaggc tagatacagt attgtattcg gttcaagact
     2521 caaggtcaga gaaggggatg tcatcgaagc cggtgacgag ttgactgaag gatctgtcaa
     2581 tcctcatgac attctaaaaa ttaaaggcat aaagggagta caaaattata ttgtaaagga
     2641 agtacaaagg gtatatagat tacagggtgt agacataaac gacaagcaca ttgagatcat
     2701 cgtgcgacag atgctgtcaa aggttaagat tgaagaagcc ggagatactg acctgttgcc
     2761 aggaagcctt gtaaattcaa tggaatttga aaaaacaaac aagaaggtcc tggaggaagg
     2821 tggagagcct gcagttggaa gaatgacact ccttggaatc accaaggcgt cacttgctac
     2881 ggactcattc ctttcagcgg cgtcattcca ggaaactacc cgagtactga cagaagcagc
     2941 aattaaaggt aaagaggatt tcctattggg tctaaaggaa aatgtaatca tcggtaagct
     3001 tatcccagca ggtaccggaa tgagaaggta caagaatgtt gaggttgtag atgacgagat
     3061 agtcccccag gaggatgaaa atttagttga cacagatatt ctgcagtgat agaatactat
     3121 agtgtgcaaa ctttagaaga ctcttgatga cataaagatt atgtggtctt ttggccacat
     3181 aatctttgat atgtttaaag gaggatagat aatgtttaca gatttgaagg aacaggacaa
     3241 ggtagtgggg gctaaacagg tcaagcgatc cctgtccaga tcagaggtcc agacggtttt
     3301 catagcccgg gatgcagaaa agaaagtaac cggggaaatt gaagaccttt gcaagtccaa
     3361 ggatgtcaag gtcatgtatg ttgacaccat ggaggaactt ggagaggcct gcggaatcga
     3421 tattaatgca gcagtcgctg cgttattaaa taaaaattag aaggaggtgc cttaatgcca
     3481 acaattaacc aactagttag aaaaggaaga gaaaaggttg tttacaaatc aaagtcgcca
     3541 gctcttggta tgaacctaaa taccttgaaa aagaaagaga ctgaatccaa ttcacctcaa
     3601 aaaagaggag tttgtgttgc tgttagaaca ataacaccaa agaagcctaa ctcagcgctt
     3661 agaaaagttg caagagtaag acttacaaac ggaatggaag tagctgccta tattccaggt
     3721 atcggtcaca atctgcaaga gcacagtgtc gttcttatca gaggcggaag agtaaaagac
     3781 cttccaggtg taagatatca catagttagg ggtactttgg ataccgctgg agttgaaaac
     3841 agactacagt caagatccaa gtatggtgct aagaagccta aggttaagaa gtaaacgaca
     3901 agaagtgctt gaaatgctaa taatacttta atatagatgc attttaaggc acaacggcaa
     3961 acgccgagta ccaatgaatc tactctatat agttaaggag ggaagaaaag tgcctagaaa
     4021 aggacatata ccaaaaagag aaatcatgcc agatccaatt tacaatgatg tggtagttac
     4081 aaagttaatc aacagcatta tgctggatgg gaaaaaagga atagcgcaga atatagttta
     4141 tggggctttt gatcaaatcg ctgagaagac aggtgaagac ccattggagg tattctacaa
     4201 ggctatgaac aacatcatgc ctgttcttga ggtaaaagca agaagaatag gtggagcaaa
     4261 ctatcaggtg cctatagagg ttagaactga tagaagagaa actcttggtt tgagatggtt
     4321 agtactatac tccagaaaaa gaggagaaaa aaccatggtt gaaaagcttg caaaagagct
     4381 tatggatgca gcaaacggtc ttggagcaag tgttaagaag agagaagaaa cacataaaat
     4441 ggcagaagct aacaaagctt tcgcacacta cagattctag taggacgttt aaaatgtgtg
     4501 gatgggattt attaattagt atgtaaggag gaaaaattgt gccaagacaa gtatcacttg
     4561 agaaaactag aaatatcggc ataatggctc atattgatgc aggaaaaaca accaccacag
     4621 aaagaatatt attttatacc ggtaaaatac acaaaatagg cgaaacccat gagggtggag
     4681 ctcaaatgga ctggatggaa caggagcaag agagaggaat cactatcacc tccgctgcga
     4741 cgacagcttt ctggaaagat cacagggtca acgttattga cacaccaggg cacgtggatt
     4801 tcaccgttga ggttgaaagg tcactgcgtg tacttgatgg agccgttgcg ttgttctgtg
     4861 caaaaggtgg agtagagcct caatctgaga ccgtttggag acaagctgac aagtatcgtg
     4921 taccacgatt ggcttttgtt aataaaatgg atatcatggg agcagacttc tttagggcgg
     4981 ttggaatgat gaaggacaga cttaaggcaa atgctgttcc aattcagctt ccaataggca
     5041 aggaagaaaa cttcacagga attgttgacc taatagatat gcacgccaga atatacaagg
     5101 acgaccttgg acaagaaatc gagattacag agataccagc tgacttgatt gatttggcca
     5161 acgagtatag ggaacttctg atagaatctg tttcagagtc agacgaagag cttatgatga
     5221 agtatcttga aggtgaggag ctgacttccg aggaaatcat gaggggtatc agaaaagcta
     5281 cccttgatgt aaagatgact ccggtacttt gcggttcatc atacaagaac aagggagttc
     5341 agattcttct ggatgctatc ctggcttacc ttccatcacc attggatgtt cctgcaattc
     5401 agggaatcct gccaggtaca gaagaggtag ttgaaagaca tgctgatgac agcgagccat
     5461 tttcagcgct tgcgtttaag atagtaacag acccatatgt tggtaagctg gcatacttca
     5521 gagtttactc aggaagtgca aaggctggaa gttatgtata taactcaacc aagggcaaaa
     5581 gggaaagaat cggaaggatt ctccagatgc atgccaacaa aagggaagag attgatgagg
     5641 tttttgctgg ggacatagct gcagcagtag gctttaagga tacatcaaca ggtgacaccc
     5701 tctgtcttga tacagatccg gtaatccttg agaagatgga attcccagag cctgtaatcc
     5761 acgtagccat tgaaccaaag acaaaagcca gccaggaaaa gatgtctgtt gcccttgcaa
     5821 agcttgcaga ggaagaccct acattcagaa cctatactga tgaggaaact ggacagacaa
     5881 tcatcgctgg aatgggagag ctacaccttg agatcattgt agacagactt cttagggaat
     5941 tcaaggttga ggcaaatgta ggtaatccac aggttgctta caaggagtca atccagaagc
     6001 cggctgatgc ggaaggtaag tatgtcaagc agtcaggtgg tagaggacaa tatggtcatg
     6061 ttaagcttaa ggttgagcca caggagccag gtaaaggtta tgagtttgtt aatgcaattg
     6121 tgggaggagc aattcctaag gagtatgtga actcagtaaa caatggtatc caggaagcac
     6181 tggaatcagg aattcttgga ggataccccg tacttgatat caaggtaaca ctttatgacg
     6241 gatcatacca cgatgttgac tcttcagaga tggcatataa aatagctggt tcaatgggag
     6301 taagggaagc tttgagaaaa gctggaccaa cacttttgga accaatgatg aaggttgagg
     6361 tcacagttcc tgaggagtac atgggagatg taatcggaga cataaactca agaagaggta
     6421 gaatggaagg catggagctt gtaagtggag ctcagataat cagagcatat gtgccattgg
     6481 cagaaatgtt cggttatgca actgacctga gatcaaacac acagggaaga ggcctttaca
     6541 caatgcagtt tgaccattac gagccagtac caaacagcat atctgagcag gtattgggcg
     6601 gaaaataatc tttaaaatag gaggagt
//
LOCUS       DASRQF010000278         6188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_276873, whole genome shotgun sequence.
ACCESSION   DASRQF010000278 DASRQF010000000
VERSION     DASRQF010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6188
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276873"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VFD79_07445"
     CDS             complement(<1..597)
                     /locus_tag="VFD79_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZH93922.1"
                     /translation="MAKNIIVIKGAKVHNLKNVSLELPRDKFIVFTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSSYARQFLGQMDKPDVEYIEGLSPSISIDQKTTSRNPRSTVGT
                     VTEIYDYLRLLFARIGTPYCPHCGKEITSQTVDQMVDHVMDLPERTKIQVLAPVIRGK
                     KGEHTKLINQIRKDGFVRMMIDGEVVDLDDDIKLDKNKH"
     gene            complement(599..2572)
                     /gene="uvrB"
                     /locus_tag="VFD79_07450"
     CDS             complement(599..2572)
                     /gene="uvrB"
                     /locus_tag="VFD79_07450"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HZH93923.1"
                     /translation="MNEFRIESSYKPTGDQPEAIKSLVKGLEEGNKHQVLLGVTGSGK
                     TFTMANVIERVQKPTLVIAHNKTLAYQLASEFKEFFPDNAVEYFVSYYDYYQPEAYVP
                     QTDTYIEKDSSINDEIDKLRHSATMSLFERRDVIIVASVSCIYGLGDPIDYENLVVSL
                     RPGMIKDRDQIMRKLVDIQYVRNDINFERGTFRVRGDVLEIFPASSSENTLRVEFFGD
                     EIDRITEVNHITGEVVGYRNHVSIFPASHFATTSEKVEKAIVRIEEELEDRLRELRDK
                     DKLVEAQRLEQRTRYDLEMLREMGFCSGIENYSRHLSDRPAGSKPYTLIDYFPDDFLI
                     IVDESHVTVPQIGGMYAGDRSRKTTLVDYGFRLPSALDNRPLRFDEFEKMVNQIIYTS
                     ATPGPYEMERTEQIVEQIIRPTGLLDPVIEVRPTEHQIDDLLAEIRRVSARGERTLVT
                     TLTKRMAEDLTDYFKEVGIKVTYLHSDVDTIERMEIIRDLRLGKYDVLVGINLLREGL
                     DLPEVSLVAILDADKEGFLRSETSMIQTIGRAARHLEGKVIMYADKITGSMRRAIDET
                     ERRRKIQADFNQEHGITPKSIVKDVRNIIEATLVAEDQIEYDEDLSAEELHAIVLGME
                     SAMLQAAESLEFERAAELRDKIIQMKKRYGGKS"
     gene            2704..4275
                     /locus_tag="VFD79_07455"
     CDS             2704..4275
                     /locus_tag="VFD79_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93924.1"
                     /translation="MKRTSGLLLIMIATFILTGCSLPMLRETSATPGKASMPEPEFTV
                     EQVVLTQGFQNTEPRIELVRKNNRPKLIVSPGILQSTGMEVSKIEKVDDVFNIHLVNS
                     SYSSADLVIPQITILLSNVSQAEADTLKFKIVNENFTPISVSHGIVDVLNKIRSDFRI
                     SSEGYPTIKLVMDDKTPTWVIDYENIYNKANLEIPLINLRLKVNANSGEVVESTKSLI
                     SSYLDEGNILNFLPGVGMLYSQKDLESENTILVYHNLATGEKTNLYRTLSEITSARLN
                     ENGTAVVLLEKEGEKLTAFTLTLEDRKAVRIGSEEGINPEHVSWTSTDEIQVITKFSD
                     NQTQVLSYNTKDGSLGVTHSFMMDLATFNVMGDTILASEFIGEETNNRILLSDGSNGL
                     RFVDVGFSPVILSEGLGMHLVNDENLTNNGLHLFNLETLENEYTLDFDIVHASRISQS
                     ELFVVERLPGKSNFGLHIFNFESREITSIGSLTTRRIYLDLASETIYANDSITYDSDA
                     KDIIYTINLNNLKKR"
     gene            complement(4302..5522)
                     /locus_tag="VFD79_07460"
     CDS             complement(4302..5522)
                     /locus_tag="VFD79_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZH93925.1"
                     /translation="MSKKKMVVIFLAILLITNLATLAIGNFVSVVVGSRVIVKKSEYE
                     ALNSVYQENAKIQQVKKVIDEMYLWEYDEDELFEGQIKGMVEALDDPYSVYMTREEYE
                     SFNMETDGIYGGIGIIVTPGEDGLITVVSPIEDTPGERAGIRTGDKIIKVEGKDYFSD
                     TMDEAVSLMRGEPNTDVQITIMRRNRDETTETFDLNITREIIRLQSVKSDVIEDDIGY
                     IRLTSFDNLTYEDFKKALMEIQSKDVKGIVLDLRSNPGGLLNVSVDIADELLGEGTVV
                     YTEDKYERRQYEESDKKMVDLPLVVLVNEGSASASEILAGAIRDNDRGSLVGTTTFGK
                     GLVQRIRDLPDGSGIKVTVSEYFTPNGINIHGIGIEPDVVVELDEEVEGIGLDYLDQD
                     NQLQKAIEVLREKL"
     gene            complement(5714..>6188)
                     /locus_tag="VFD79_07465"
     CDS             complement(5714..>6188)
                     /locus_tag="VFD79_07465"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZH93926.1"
                     /translation="DKLNDFAKEIESKIVKLQRNTGPYSGGKMDWPVPGHSRISSPYG
                     YRIHPIFKVKKLHTGIDIPAPTGTPITAAAAGTVIYSDWLGGYGKVVMLDHGGGIVTL
                     YAHNSALLVSEGQTVARGATVAKAGSTGNSTGPHLHFEVRKDGSYVDPIPWLRGN"
BASE COUNT         1567 a         1539 c         1197 g         1885 t
ORIGIN      
        1 atgcttattc ttgtccagtt ttatgtcatc atccaggtcg acaacttcgc catcaatcat
       61 catcctgaca aaaccatctt tccttatctg attgataagc ttggtgtgtt ctcccttctt
      121 acctcttatt acaggtgcca gtacctgtat ttttgtcctt tcaggcaggt ccataacatg
      181 gtccaccatc tggtcaacgg tctggctagt gatttcctta ccgcaatgtg gacagtatgg
      241 agtgcctatc ctggcaaaga gaagtctcaa gtagtcatat atctctgtga cagtaccaac
      301 cgtggacctt ggatttctcg atgttgtttt ctggtcaatg gatatggacg gagaaagtcc
      361 ctcgatgtac tccacatcag gcttgtccat ctggccgaga aactgtcttg catagcttga
      421 cagggactcc acatatctcc tctggccctc ggcgtagatt gtgtcaaagg caagggaaga
      481 cttccccgac ccgctcaatc cagtgaaaac tataaattta tctctgggaa gctcaaggct
      541 gacattcttt agattgtgaa cctttgctcc ctttattact atgatgtttt tagccatttc
      601 aactctttcc tccgtacctc ttcttcattt gaattatctt gtccctcagc tcggcagccc
      661 tttcaaactc aagggactcc gctgcctgaa gcattgcact ttccataccc agcacaattg
      721 catggagctc ctccgctgac aggtcctcgt cgtattctat ctgatcttcc gccacaaggg
      781 tggcttcaat tatgtttctt acatccttga caatagattt aggtgtgatt ccgtgctcct
      841 gattgaaatc tgcctggatt tttctacgtc tttcagtctc gtcgattgcc cgtctcatgg
      901 accccgtaat cttgtcagca tacattataa cctttccttc aagatgtctt gcggccctac
      961 ctattgtctg aatcattgat gtctcacttc tcaggaagcc ctccttgtct gcatccaaaa
     1021 tggcaaccag tgaaacctcc ggaaggtcaa gaccctcacg gagaaggttt atccctacca
     1081 ggacgtcgta cttgcccagt ctcaggtccc taattatctc catcctttca atggtgtcca
     1141 catcggagtg gaggtaggtt accttgattc caacctcctt gaaataatcc gtcaaatcct
     1201 cagccatcct tttggtcagg gttgtaacaa gggttctctc ccccctggct gacactctcc
     1261 tgatttcagc aagaaggtca tcgatctggt gctctgtggg tctcacctct atgacgggat
     1321 ccaacaaacc agtaggtcgg atgatctgct ccactatctg ctctgtcctc tccatctcat
     1381 agggtcctgg agttgcagat gtatagatta tctgattgac catcttttca aactcgtcaa
     1441 acctcagtgg cctgttgtcc agggctgatg gaagcctgaa tccgtaatcc accagggtgg
     1501 tcttcctgga cctgtctccg gcgtacatcc cacctatttg aggcactgta acgtgtgact
     1561 catccacgat gataaggaaa tcgtccggga agtagtctat cagtgtgtat ggcttgcttc
     1621 cagctggtct gtcgctcaga tgtctggaat agttctcaat acctgagcag aagcccattt
     1681 cccttaacat ttccaggtcg tacctggttc tttgctcaag tctttgagcc tccacaagct
     1741 tgtccttgtc cctcagttcc ctaagcctat cctcaagctc ctcctctatt ctgacgattg
     1801 ctttttccac cttttcagag gttgttgcga agtgggaagc cgggaaaatt gaaacgtggt
     1861 tcctatagcc aaccacctca cctgttatgt ggttcacctc ggttatccta tctatttcat
     1921 ccccgaaaaa ttcaacccta agggtgtttt ccgatgagga tgcggggaaa atctccaaca
     1981 catcccccct taccctaaag gttccccttt caaagttaat gtcatttctc acatactgaa
     2041 tatccaccag ctttctcatg atctggtccc tatccttgat catgcccggt cgcagtgaaa
     2101 ccacaagatt ttcgtagtca atggggtctc ctagaccgta tatgcatgaa actgaagcaa
     2161 ctataatcac atcccttctt tcaaagagag acatggtggc tgagtgccta agcttgtcta
     2221 tttcatcatt tatggatgaa tccttttcaa tgtatgtgtc tgtctggggc acataggcct
     2281 caggttgata atagtcatag tagcttacaa aatattcaac ggcattgtct ggaaaaaatt
     2341 ccttgaactc acttgccagc tggtaggcca gggtcttgtt gtgggctatc actagagttg
     2401 gtttttgaac cctctcaatt acatttgcca tggtaaaggt ctttcccgat cctgtaactc
     2461 ccagaagcac ctggtgtttg ttgccttcct ccaagccctt gaccaatgac tttattgcct
     2521 ctggttgatc accagtgggc ttgtatgatg attctatcct aaattcattc ataaatccac
     2581 ctcattcctg aacacttgtt ccctttatat gatactattt ttccatgaaa aaatccacct
     2641 taattattaa cttgtggaaa gcatcccttt aatgtaaaat tatccatagg aggcgattcc
     2701 ataatgaagc gtaccagcgg acttttgctg ataatgatag cgacttttat tttaacaggc
     2761 tgctccttac ccatgctaag ggaaacttca gccactccag gaaaggcttc aatgcctgag
     2821 cctgaattca ctgtagagca ggtggtacta acacaaggct tccagaatac tgagccaagg
     2881 attgaactgg tccgtaaaaa caaccggcca aaattgattg tttcacctgg aatacttcaa
     2941 agcaccggca tggaagtatc caaaatcgaa aaagttgacg atgttttcaa tatacacctt
     3001 gtcaatagct cctactcctc tgcagattta gttatacccc aaattaccat tttgctttca
     3061 aatgtaagcc aggctgaagc ggatacattg aaatttaaaa ttgttaatga aaatttcact
     3121 cccatctccg tcagccatgg aatagtggat gtacttaata aaatccgctc agatttcagg
     3181 atatcaagtg aggggtaccc caccatcaag ctggtgatgg atgataaaac cccaacttgg
     3241 gtgattgatt acgaaaacat atacaacaag gccaacctgg aaataccact gatcaatctt
     3301 aggctgaagg tcaatgcaaa ttcgggagag gttgtggagt caaccaagag cctgatttct
     3361 tcatatttgg atgaagggaa cattctgaac ttcttacctg gtgtagggat gctttactcc
     3421 cagaaggacc ttgaaagtga gaacacaata ctggtttatc acaatctggc cactggagaa
     3481 aaaaccaacc tatacaggac cttatcagaa attacttcgg caagactgaa tgaaaatggc
     3541 actgcagtag tattactgga aaaagaaggc gagaaactga ctgccttcac cttgaccttg
     3601 gaggacagaa aggcagttcg aatcggatct gaagaaggca taaatccaga gcatgtctcc
     3661 tggacaagca ccgatgaaat acaagtcata acaaaattct cagataacca gacacaggtc
     3721 ctttcctaca atacaaagga tggcagctta ggtgttacac acagcttcat gatggatctg
     3781 gcaacattca atgtcatggg tgatacaatc ctggcatctg aatttatagg tgaggaaacc
     3841 aacaacagga tcttgctgtc ggacgggagt aatggcctta gatttgtgga tgtgggcttc
     3901 agtccggtta tactttcaga aggactgggt atgcaccttg taaacgacga aaatttaacg
     3961 aataatgggc ttcacctttt taaccttgaa acccttgaga atgaatatac cttggatttt
     4021 gacatcgttc atgccagcag aatatcccag agtgagctat ttgtagttga aagacttcct
     4081 ggaaaaagca actttggact acacattttt aactttgaaa gcagggagat cacctcaatt
     4141 ggtagtttga ccacccgaag aatatatttg gatctggctt ccgaaaccat ttatgcaaat
     4201 gattccatca catatgactc tgacgcaaag gatataatct ataccataaa cctgaataat
     4261 cttaaaaaga gatagcctca tattggctat ctctttctta tctaaagttt ttctcttaag
     4321 acctctattg ccttctgaag ctggttgtcc tggtcaaggt agtccaagcc aatgccttcc
     4381 acttcctcat caagctctac aaccacatcc ggctcaattc ctatcccgtg aatgtttatt
     4441 ccattgggag tgaagtattc cgatacggta acctttattc ctgaaccatc tggcaggtct
     4501 ctgattcttt gtacaagtcc ctttccaaat gttgtggtcc caacaaggct acccctgtca
     4561 ttatccctga tggccccggc gaggatttct gatgcacttg cgctgccctc atttacaagg
     4621 accaccagtg gaagatccac catttttttg tccgactcct catactgtct tctttcatac
     4681 ttatcttcgg tgtaaaccac agttccctct ccaaggagtt catccgctat atccacgctg
     4741 acattgagca ggcctcccgg gttgcttcta aggtccaaaa caattccctt cacatctttt
     4801 gactgaattt ccatcaaggc cttcttgaag tcttcgtagg tgagattgtc aaaggatgtc
     4861 aatcttatat atccaatatc atcttcaatc acatcgcttt tcactgattg aagtctaatg
     4921 atctccctgg ttatgttcaa gtcaaaggtt tcagtggttt catccctgtt ccttctcata
     4981 attgtaatct gaacatcagt attcggctct ccgcgcatga gacttaccgc ctcatccatg
     5041 gtgtcagaga aataatcctt gccttcaacc tttattatct tgtcaccggt ccttattccc
     5101 gctctctctc caggggtgtc ctctatgggt gatactactg ttatcagccc atcttctcca
     5161 ggggtgacaa tgatcccaat gcctccatat attccatcgg tttccatgtt gaagctctcg
     5221 tactcttccc tggtcatgta gactgagtat gggtcatcca aggcctccac cattcctttt
     5281 atctggccct caaagagttc atcctcgtcg tactcccaga gatacatttc gtcaatgacc
     5341 tttttgacct gctgtatttt tgcattttcc tggtatacag aattaagtgc ctcatattcg
     5401 ctttttttaa caataaccct gcttccaacc actactgata caaaattccc aatggccagt
     5461 gtggccagat tggtaatcag cagtattgca aggaagatca ccaccatttt tttctttgac
     5521 atttccccac gtataatcca attaacctct ttaatatagg gggttaattg gattatttct
     5581 cctttctccc aaattatttg ggtattatcc attactccgt tattataatg ataaagcact
     5641 gtggatttgt tactatttcg ctacgggttt gttttttgac taatttaaaa aagaggagat
     5701 ttctctcctc ccattaattt cctcttaacc aaggtattgg atccacgtat gaaccgtctt
     5761 tcctaacctc aaaatgtagg tgtggtccgg ttgaattacc agtgcttcca gccttggcaa
     5821 ccgttgcccc tcttgccaca gtttgccctt cggagacaag cagtgctgaa ttgtgtgcat
     5881 acagggtaac tattccgcca ccatggtcca acataaccac cttaccataa ccgcctagcc
     5941 aatcgctgta gattactgtc cctgctgcgg ctgcagttat tggtgttcca gttggtgcag
     6001 gaatgtctat acccgtatga agcttcttga ccttgaaaat ggggtgtatc ctgtacccgt
     6061 atggagaact gattctactg tgtcccggta cgggccaatc catcttccct ccagagtatg
     6121 gtccggtatt cctctgaagc tttactatct tgctttcaat ttccttggca aaatcgttca
     6181 gcttatca
//
LOCUS       DASRQF010000279         4026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_416016, whole genome shotgun sequence.
ACCESSION   DASRQF010000279 DASRQF010000000
VERSION     DASRQF010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416016"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VFD79_07470"
     CDS             complement(<1..446)
                     /locus_tag="VFD79_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93927.1"
                     /translation="MKRLVQADTKKLKKELLGFYKTQYRNHPLKRDSMSGLLEGILFE
                     KSQLSKAVKAHPLMVMEGEEVVIAAVLAHVDRMADYLQIAFFESPSQDLEAFDLLMDQ
                     ANLLSKEYGCKKICASLNIHVNYGLGYLLDSYDKPQSFGMAHNPPH"
     gene            570..1241
                     /locus_tag="VFD79_07475"
     CDS             570..1241
                     /locus_tag="VFD79_07475"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZH93928.1"
                     /translation="MDINIIFEDEDIVVVEKPPGMPCQRDKTGDLDLMTALDRDYLGL
                     VHRLDRPVGGVMVYARKESSNTWLSKGIRDRAFHKEYLAVVIGRPSEEKGQMVDQLKK
                     LRTVNMSKVVSEDVSGSKKAILEYRLLQSVETEEHGALSLVHIVLLTGRHHQIRVQLS
                     NAGLPLWGDTKYNGAFTNRKDWTQVSLWSHRIKFRHPGGRKVSYHSLPEDAYPWNLFE
                     MPEIK"
     gene            1257..1808
                     /locus_tag="VFD79_07480"
     CDS             1257..1808
                     /locus_tag="VFD79_07480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZH93929.1"
                     /translation="MKEIYHTDRLILRILRPKDAGLVLDYITRNKDFLKEWETDRPDD
                     YYTLGHQKSLLKRDYRRFQEGQMLRLWIFRKDDPSKVIGTIALDNIIRGPFLSCFLGY
                     RLDRDNINQGYTTEALGELIRIAFEDMGLHRLEANIMPRNLRSLRVVEKLGFVDEGLS
                     RDYLKINEKWEDHIHMVLLNKDL"
     gene            complement(1818..2390)
                     /locus_tag="VFD79_07485"
     CDS             complement(1818..2390)
                     /locus_tag="VFD79_07485"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZH93930.1"
                     /translation="MLKDLVLNNRTYRRFYGEEKISRETLLDLIELARLSSTGANLQQ
                     LKYIISNTPEKNQMIFQELKWAAYLKDWDGPVEEERPSAYLVMVADKEIGSNIYWNHG
                     IACQSILLGATEKGLGGCMFGGFNKENLKKKLQVPDRYEILLVIALGKPKEEVVLEDL
                     GPEGDIKYWRDEKGVHHVPKRRLEDLILDL"
     gene            complement(2430..2735)
                     /locus_tag="VFD79_07490"
     CDS             complement(2430..2735)
                     /locus_tag="VFD79_07490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04104 family putative zinc finger protein"
                     /protein_id="HZH93931.1"
                     /translation="MKVQKCINCGTQFRYKSVQKSIWAGYKPIICFNCGAIHEFRFVY
                     RLVVSVLIVLPIFFNQWISDIAPNPLVHSAMYFGYIFVVIGIMPYLIRYRLKNDNLE"
     gene            2959..3930
                     /locus_tag="VFD79_07495"
     CDS             2959..3930
                     /locus_tag="VFD79_07495"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HZH93932.1"
                     /translation="MIRIDDSARIVGKTRLGKDAYIAQGTMVRSENDSVILGNKTWAL
                     ENSVLIGTEAHPLRVGSKTVFGHKCIVVGAEIGDLCEIGNGAIFLPGSKIGNMCIFGE
                     GTLIPEGTEIPDESVVVGRPGRVIRKLTTDDRDMIKRMRGGDISLSPFEENIVDNNGK
                     ESEIMGKLYTVNGKYPQVSDSAYIYDTAEINGDVKIGDGSVVSSGVRIIGNTHGPVVI
                     GNNVQILENTVLHLLPDNQLVIEDDVTIGPGCIIHGTTIGRASVVESGAIVCDYSRLG
                     ENTLVKSGSLVKQRSEFGDNEILEGFPAKSIGSNEAELKRPDWAIKP"
BASE COUNT         1126 a          852 c          956 g         1092 t
ORIGIN      
        1 atatgaggtg gattgtgggc catgccaaag ctttggggct tgtcgtagct gtccaagaga
       61 tatcccagac cgtagtttac atggatgttg agggatgcgc agattttttt gcagccatac
      121 tccttggata gaaggtttgc ctggtccata agcaaatcaa aggcctccaa atcctgactg
      181 ggtgattcga aaaatgctat ctgcagataa tcagccatcc tgtccacatg ggcaagcact
      241 gctgcaatga caacctcttc accctccatc accattaggg gatgggcctt aaccgccttt
      301 gatagctgtg atttttcaaa tagaatccct tccagaaggc ctgacatgga atccctcttt
      361 agaggatggt tcctgtactg agtcttatag aaaccaagga gttccttttt tagttttttt
      421 gtgtctgctt ggacaagtct tttcatctaa tacctctttt ctctcccgtt tattatacca
      481 tagggtattt cctatttccc attacaatga tgtaacctct ttgtatttgg tgtataatct
      541 agctatgaat aataatcctg gaggtagcga tggatataaa tataattttt gaggatgaag
      601 atatagttgt tgttgaaaag ccccctggca tgccatgcca gagagacaag accggagact
      661 tggaccttat gactgccctt gacagggatt atcttggtct tgttcacagg ttggaccgac
      721 ctgttggcgg agtcatggtc tatgcaagga aggaaagctc aaacacctgg ctgtcaaaag
      781 ggatcaggga cagggccttt cacaaggaat acctggcagt tgtcattggc aggccatcgg
      841 aggaaaaggg ccaaatggtg gaccaactaa aaaagctccg gacagtcaat atgagcaagg
      901 tggtttcaga ggatgtaagt ggttcaaaga aggcaatcct tgaatacaga cttctgcagt
      961 ctgtggaaac agaagagcac ggagccttga gccttgttca cattgtactc ctgacgggaa
     1021 gacaccatca gataagggtc cagctgtcaa atgcgggcct ccccctttgg ggtgatacaa
     1081 aatataacgg ggcgttcaca aatagaaagg attggaccca ggtatcactt tggtcccaca
     1141 ggataaagtt cagacatccc gggggaagaa aggtctccta ccattccctt ccggaggatg
     1201 cttatccctg gaaccttttt gaaatgcctg aaatcaaatg atgtggagga ataaggatga
     1261 aggaaatata ccatacagac aggttgatac ttaggatact cagacccaag gatgctggac
     1321 ttgtccttga ttacatcacc agaaacaagg atttcctcaa ggagtgggaa acagacaggc
     1381 ctgatgacta ctataccctc ggtcaccaga aaagcctcct gaaaagggac tacagaaggt
     1441 tccaagaggg acagatgctg aggctgtgga tattcaggaa ggatgatcct agtaaggtaa
     1501 taggtaccat tgcccttgat aatataataa gaggaccatt tctctcatgt tttcttggct
     1561 acaggcttga cagggacaac attaaccaag gctacacaac tgaagccctt ggagagctta
     1621 tccgaatcgc ctttgaggac atgggccttc acaggctgga ggcaaacatt atgcctcgaa
     1681 acttgaggtc ccttcgggtt gtggaaaagc tgggctttgt agatgaggga ctttccagag
     1741 actacctgaa gataaacgaa aaatgggagg accacataca tatggtcctc ctgaataaag
     1801 atctctaatt gtaggtccta taggtccagt atcaggtcct caagccttct ctttggaaca
     1861 tggtggactc ccttttcatc tctccagtac ttgatgtcac cctctggccc aaggtcctca
     1921 agtacaacct cctcctttgg tttgccaagg gcaattacaa ggaggatttc atacctgtcg
     1981 ggtacctgaa gcttcttctt caggttttcc ttgttgaagc ccccgaacat acagccccca
     2041 agcccttttt ccgttgctcc caggagtatg ctctgacatg ctattccatg attccagtat
     2101 atgttgctgc caatctcctt gtcggctacc ataacaagat atgcagaggg cctctcctcc
     2161 tcaactggtc catcccagtc cttcaggtat gcagcccact tgagctcctg gaatatcatc
     2221 tggtttttct caggggtatt ggatattatg tacttgagct gctggaggtt tgctcccgtt
     2281 gatgacagcc tggcaagttc aataaggtcc agcaatgtct ccctgctgat cttttcctcc
     2341 ccataaaatc tacggtaggt cctgttattt agtaccagat cctttagcat tttatccctt
     2401 cctttcatac tgattgttct atctaagctt cattccagat tgtcattctt caggcgatac
     2461 ctgatcaaat atggcatgat ccctatcacc acaaatatat acccaaaata catggcagag
     2521 tgaaccaaag gattgggcgc aatatcactg atccattggt tgaagaatat gggtaggact
     2581 atcagcacag aaaccaccag tctgtataca aacctgaatt catggatggc tccacagttg
     2641 aagcatatga taggcttgta gcctgcccat attgatttct gaactgactt gtacctgaat
     2701 tgggtgccgc agttgatgca tttttgaacc ttcatccaag acctcctaac catagctcct
     2761 attgtatatt gctgattgtt gcactcattc tcatactacc caatttattt atatcttcaa
     2821 cacctgagca tgagcttggg agcaaaatgt tgttggaaat gatccattat tatggagagt
     2881 tgaaattcat ctgttatttc actgggataa aaggtaaaat aaggataagg agcaagcatt
     2941 aaaaggaggc tcgatgatat gattcgaatt gatgattcag caagaatagt gggcaagaca
     3001 agacttggca aggacgccta catagcacag gggacaatgg taagatcaga aaacgactcg
     3061 gtcattcttg gcaacaagac ctgggctctt gagaattcag tgcttattgg tacagaagcc
     3121 catccgctca gggttggaag caaaaccgtg tttggacaca agtgtattgt tgtcggggct
     3181 gaaattggag atttgtgcga aattggcaac ggggcaatat ttctgcctgg ttcaaagata
     3241 ggaaatatgt gtattttcgg agagggtact ctgataccgg agggaactga gattcctgat
     3301 gagtcggttg ttgtaggcag gccaggccgg gtaataagaa aactcacaac tgacgacagg
     3361 gatatgatca aaaggatgcg gggaggagac atctcattat ccccctttga agaaaacatt
     3421 gtggataata atggcaagga gagtgaaata atgggcaagt tatacacagt caatggcaaa
     3481 tacccccagg tttcagattc agcatacata tatgatacag ctgagatcaa tggagatgtt
     3541 aagataggtg atggttcggt tgtttcatca ggtgtgagga tcattggcaa cacccatgga
     3601 cctgtagtga ttgggaacaa tgtccagata cttgaaaaca cagtacttca tctcttgccg
     3661 gacaaccagc tggtcataga ggatgatgtg accatcggtc cgggctgtat aattcatggt
     3721 acgaccatcg gcagggctag cgtggtggaa tcaggagcca ttgtatgtga ctacagtagg
     3781 ttaggtgaaa ataccttagt taaatcaggc tcacttgtaa agcaaagaag tgaatttggt
     3841 gacaatgaaa tacttgaggg ctttccagct aaaagcattg gaagtaatga agcagaactg
     3901 aagagaccgg actgggccat taagccatag gtctatccaa aaattgaagg gtcagcatgg
     3961 atttttccat actgaccctt tttttatgac aggctgagta tctttcttgc ctcatctgtt
     4021 gttgcg
//
LOCUS       DASRQF010000280        13328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_168604, whole genome shotgun sequence.
ACCESSION   DASRQF010000280 DASRQF010000000
VERSION     DASRQF010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 13328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13328
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168604"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /gene="radC"
                     /locus_tag="VFD79_07500"
     CDS             <1..635
                     /gene="radC"
                     /locus_tag="VFD79_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HZH93933.1"
                     /translation="LLAMGPGALSNSELLAVIIRTGTGSETAIEVSRRLLSMDSRGVA
                     FLADASIGDLTDVKGIGNCKAAQIMAAIELGKRMKRLNPYDKIRVTSPDVVANLVMED
                     MKLLQKEHFNIAILDTKNQIISIDNISIGTLNASIVHPRDVFNAAIRKNANSVILLHN
                     HPSGDVNPSNEDINITKRLIEAGNIIGIKVLDHIIIGNDVFLSMKEKNFM"
     gene            651..1682
                     /locus_tag="VFD79_07505"
     CDS             651..1682
                     /locus_tag="VFD79_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HZH93934.1"
                     /translation="MVLFKGLTKDMGIDLGTANTLVYIKGKGIILREPSVVAIDRNTK
                     QVLAVGEDAKKMIGRTPGNIVAIRPLKDGVIADFDITQEMLKYFIRKATQRKSVFQPR
                     VVVCVPSGVTEVEKRAVEEATIQAGAKEAFLIEEPMAAAIGAGLPVHDATGSMVVDIG
                     GGTTEVAVISLGGIVTSKSIRVGGDELDTYIVNYVKKEYSLMIGERTAEEVKLGIGSA
                     INDPELGTMEIRGRDLVSGLPKTLEISSKEIYEALREPVNNIIDSIKSTLEKTPPELS
                     SDVMEFGIVLTGGGAMLNGLDSLITKETGIPVFVADDPLDCVANGTGKALDSIEILKK
                     TSSRNNRLR"
     gene            1690..2526
                     /gene="mreC"
                     /locus_tag="VFD79_07510"
     CDS             1690..2526
                     /gene="mreC"
                     /locus_tag="VFD79_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HZH93935.1"
                     /translation="MHFLRKYKERMIVIAIAIILLIVIGQTSKERISLSGIERLTGNI
                     LSPLTSGVTTAREGIAGFFSSIGETFEAREENELLKDEIKRLQSENRNLLDIIGKTDY
                     LRNEMELERATELTLLKGKVVGKEPGNWFSRFTINLGSKDGVIKGATVVQGVEVEQQL
                     YQEGIVGRVVDVGDNWAKVTTVIDELNSVSFKIIRTQDGGVLSGGLDGQLEGYLFDFE
                     ADVIVGDKLYTSGLGGVYTQDIYIGEVTEIRTSQEELTKTIVVQPAVDFRKLYNVFII
                     VN"
     gene            2540..3040
                     /gene="mreD"
                     /locus_tag="VFD79_07515"
     CDS             2540..3040
                     /gene="mreD"
                     /locus_tag="VFD79_07515"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HZH93936.1"
                     /translation="MTILLITMSIILNIIIQATLIPNIGVFGVVPNTALVLVVMIALK
                     KGRIYGSAFGLLAGLLQDVLFSITIGVNAFILFFIGYFVGFAYNSLTRESGINHFIFT
                     ALSTVFYNVLFSLIQFFLSRDVSFVDAVQSIFSLEVVYNGIIAVLLFMLIQRLFTQPN
                     LKFGRR"
     gene            3047..6301
                     /locus_tag="VFD79_07520"
     CDS             3047..6301
                     /locus_tag="VFD79_07520"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZH93937.1"
                     /translation="MKLREKLFNRYSLMIYFLVLITTMLLFRLATLTIAQGDYYRDLS
                     DNKRVKEVYTTAPRGEIRDRHGRLLAGNVPTFTVQLLKDELNFGDRTVRNDSLLQLVR
                     LLESDGTEYANDYPVELNLIKYRSIDSYLTNEVGPMDKLVIQILENNLLNEFLNMEFS
                     KPYNDHFGLSIFKRAQDILSDKNPEIYSLDPGNEEDVLKISQLLQDDKVNTRKIVDHP
                     VARLLAYDLLRKYDLQEDLALEEYALTYYEDYIQQKADLMDDYPYITMDTSGEEDFIN
                     LFSEIGLYSFLSRAIPLGGETEEYLVPGEVLIEYLEENGGSSPIEIRIGDNGTDLLYS
                     FVDTTGISDQDPLDHLIGLAEEMNLLAGFIKDERIRYQAQSHLINSGINPRISVAGDI
                     EYVHINNMNNWYEANKVEKGTPLDEAFMDVRRIYSINESISPYEARALLGLINQLSIQ
                     GYLAYQPINIAYGIKDETVARIEEGLSGVPGINVSIEPVRVYPEGTTASHILGYIGKI
                     SQPFEIEKYIREEGYSPGTLIGKTGIEESYELQLSGVSGIRKVEVDVVGNTTNVIEEI
                     SPVPGDNIYLSIDLKLQKTAEEAMKHTLDQLSKGGTFKSEWGDYTFGTHRRKGRPYIN
                     ANSGAVVVLDVKTGETLAMVSYPSFDPNLFSTGINNTDWLSLFPEDADNPLAARPLYN
                     IATQTAVQPGSTFKMVTGLTALEKGLNPLLRIRDMGYVTVGDKRFHCLIYTNTGGTHG
                     YENFYEALSDSCNYYFYTLALGRNQRTGQDIGVKIEIEDIVEMSKKLGLNDKTGIEIN
                     IPSEVSGGVPDPQRKIITTKYLLKQMLQRSMEGYIMDGHEFSEEDIQLAIDEIISWTE
                     LEETLSRAEVVRRLTGMGIEPEMRLAGEREGLADKIKYTYLNQAGWNISDTLNVTIGQ
                     GQSAYTPIQMANYIATIANGGYLNRVTLIDSIRNYNNVSVLYEHKPERELIGLNDYEN
                     LEHVKQGMEKVADDGTARRIFQNFPVPVGVKTGTAERSGINPSTGDTYDDFAWFVGFA
                     PYDDPEIAVAAVLFQGGSGGYAAPMVREIIAEYLGLNDVEQIDTLPMENSIVFD"
     gene            6384..7031
                     /gene="minC"
                     /locus_tag="VFD79_07525"
     CDS             6384..7031
                     /gene="minC"
                     /locus_tag="VFD79_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HZH93938.1"
                     /translation="MRKDVVSIKGVAGGIYLNVEGNDLGQIKTELESKMKTASNFFKG
                     SKFLGVRGEDLTEENRVEIMLLLKYKYDFEIQIEKVPDHVVKDNYEPVKEMIAAEPEV
                     VQEGMTKFVHGTLRSGQEVSYNGNIVVIGDVNPGALLKAKGNIVVLGSLRGVAQAGIG
                     GNRHAVVAAYSLLPTQLRIADYIVRPPEDDIQYKYPEIARVVDGAVVIEPYLPNK"
     gene            7062..7859
                     /gene="minD"
                     /locus_tag="VFD79_07530"
     CDS             7062..7859
                     /gene="minD"
                     /locus_tag="VFD79_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HZH93939.1"
                     /translation="MGTAIVITSGKGGVGKTTTSANIGTGLAILGYSVVVLDADIGLR
                     NLDVVMGLENRIVYDIVDVVEKNCKLKQALIKDKRYEKLYLLPAAQTKDKTAVKPEQM
                     VELVGELKKTHDYIIIDSPAGIEQGFQYSIAGADRGIIVTTPEISAVRDADRVIGILE
                     ANELHDPQLIINRIRPDMVKRGDMMNMDDIIDILAIDLIGIVPDDESIVISTNKGEPV
                     VMDEKALAGKAYRNIVRRITGEEVELLNLEENDESKFSKVLKLIFGK"
     gene            7874..8155
                     /gene="minE"
                     /locus_tag="VFD79_07535"
     CDS             7874..8155
                     /gene="minE"
                     /locus_tag="VFD79_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HZH93940.1"
                     /translation="MDFFKMFTRKKENSKNVAKERLKLVLVHDRSDLSPRFLEMIKGD
                     IVKVISQYAEIDESEIEIKMTKMKKEGNKSPVSALVANIPIVNVKDKKK"
     gene            8272..10086
                     /locus_tag="VFD79_07540"
     CDS             8272..10086
                     /locus_tag="VFD79_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009431595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HZH93941.1"
                     /translation="MKVEKPSRYIGMEQNMVQKNPDEVDVSFAFSFPDVYEVGMSHLG
                     MHILYNLINNESRYSCERVFAPWVDMEAQMRKESIPLFTIESKEEVKNFDFLGFTLQY
                     EMSYTNILNILDLSGIPIHSRDRGGEFPLVIAGGPCAYNPEPLWEFIDLFVIGEGEEV
                     TLDLIKLYDKHKKKGYDKREFLEEAQATMEGIYVPSLYDVNYNEDGTIRDRFPWVSGL
                     PETISKRMIRDLDSMFSPEKMLVPYMETVHDRISMELFRGCTHGCRFCQAGMIYRPIR
                     EKSVDRLMELAEKLVDSTGYENISLSSLSSCDYSQLQLLISSLMDRFEDKKVGVSLPS
                     LRLDSVSIDILKEIEKVRKTGLTFAPEAGSQRLRDVINKGVDEEDLVNAVGYAFKEGW
                     SRIKLYFMIGLPTETEDDVLGIKELAYLVKDLFFNRPPEERKGNFSVTASASCFVPKP
                     FTPFQWHGQDTIEEFQAKINLVKGSIKDKKVTFNYHDPRVSYLEAIVARGDRKTSKVI
                     YKAWEKGCRFDGWSEHFKYETWLEAMDELGIDGSFYANRNREFSENLPWDFINPGPSK
                     KYLQNEYTKAIKGETTRDCRLGCTGCGIQDCAMWGVFN"
     gene            10083..10784
                     /locus_tag="VFD79_07545"
     CDS             10083..10784
                     /locus_tag="VFD79_07545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HZH93942.1"
                     /translation="MILRTRFKKIGYLKYLSHLDLVRLFTRTFSKAGIPIKFSEGFNP
                     HPKFSIGNPLPLGTESLAEYMDVELAEVIKPEIFMERMNKALPEGIQLVDCRLIEKGP
                     SLTSIIAWSHYDIRFVSEQSGEAIDVEGALSRWNKLSEVYISKKKKKGKKKVERQLDI
                     LPLIGNLNYKGMDSDGFHIISGLLKSGESGNLKPIELIEAMDRELELNIDLDMVMIKR
                     LEVFADEDGEIKELV"
     gene            10796..12031
                     /locus_tag="VFD79_07550"
     CDS             10796..12031
                     /locus_tag="VFD79_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HZH93943.1"
                     /translation="MNTIYIDSRDGVNRICIVEDGRLVEYYKEDTGRHRLLGNVYRGR
                     VVNILRGMEAAFVDIGEDRNAYLNVKDALGREMMYSKEKLTIDQVLKQGEDIIVQVVK
                     EPLGNKGPKVTTHLSIPGRYMVLTPYSNRVNISKKIRDQDEIERLRHLGKEIERERMG
                     MIFRTVSQGVESDLLREEYNDLVRKFEAIEGERNYLPTPKLIYSEPVLVLQILRDYFD
                     ESKTDVVTNSRDILEYLKTTTKLRDYNLGDRLTYDPNYNMDYHMNIQMDMKEALSRMV
                     QLKSGGYIVIDETEALTAIDVNTGKYVGNLTLSDTIVRTNIEAAVEIARQVRLRDIGG
                     IIIIDFIDMKVQDNIDIVLGKLDKEFRKDKNKPYVVGMTKLNLVEVVRKRNRPTLDKE
                     TTIVCPTCNGRGRIRKNEA"
     gene            12140..12451
                     /gene="rplU"
                     /locus_tag="VFD79_07555"
     CDS             12140..12451
                     /gene="rplU"
                     /locus_tag="VFD79_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HZH93944.1"
                     /translation="MYAIIETGGKQYKVQEGDVVFVEKLNAEEGDAVNFDKVLLMSTD
                     SGVTPGKPFVEGAKVEGTVLAQGKAKKIVVFKYKAKKNIRKKQGHRQPFTKVQINKIV
                     G"
     gene            12454..12813
                     /locus_tag="VFD79_07560"
     CDS             12454..12813
                     /locus_tag="VFD79_07560"
                     /inference="COORDINATES: protein motif:HMM:NF016233.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HZH93945.1"
                     /translation="MIKVIIGTDNTGNIESFEMSGHAEYGPYGKDIVCAAASVLGYTA
                     LRAMVEVAGVDEGSLDYKVDDDMGYMKVNLGTELENGNLSDVQVILRTYEVGIRSMIE
                     SYPKYITLEYRGGGTRV"
     gene            12806..13096
                     /gene="rpmA"
                     /locus_tag="VFD79_07565"
     CDS             12806..13096
                     /gene="rpmA"
                     /locus_tag="VFD79_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HZH93946.1"
                     /translation="MFKLNLQLFASKKGVGSSKNGRDSESKRLGVKRGDGQFVLAGNI
                     LVRQRGTKIHPGNNVQIGGDDTLFATVDGVVKYERKGRDKKQVSVYPREELA"
     gene            13244..>13328
                     /locus_tag="VFD79_07570"
     CDS             13244..>13328
                     /locus_tag="VFD79_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93947.1"
                     /translation="MFIDVARISVKAGKGGNGIVAWRREKYE"
BASE COUNT         4293 a         2294 c         3378 g         3363 t
ORIGIN      
        1 aactgttggc aatggggcca ggtgctcttt caaacagtga gcttttggct gttataatca
       61 ggactggaac aggtagcgaa actgcaattg aggtttctag aagactcctg tccatggata
      121 gtaggggagt tgcctttctt gctgacgcat caataggcga cctgacagat gtcaaaggaa
      181 tcggtaattg caaggcagca caaataatgg cagccattga actgggcaag aggatgaaaa
      241 gattgaatcc ctatgataag attagggtaa catcgccgga cgtggtggct aatttggtaa
      301 tggaggatat gaagctactg caaaaggaac attttaatat agctatactt gacacaaaaa
      361 atcagataat aagcatagac aatatctcaa ttgggacact gaatgcatca attgtccatc
      421 caagagacgt ttttaatgcc gcaataagga agaatgcaaa ctcagtgatt cttctacata
      481 accaccccag tggtgatgtg aatccaagca atgaggacat caacataaca aagagactca
      541 ttgaagctgg aaatatcatt ggaattaagg tcttggacca tataataatt ggcaatgatg
      601 tctttcttag tatgaaagag aaaaatttta tgtaattatg gaggagtaaa atggtattat
      661 ttaagggctt gacaaaggat atgggaatag atttaggtac tgcaaataca ctggtctata
      721 tcaagggtaa gggtataatt ttaagggagc catcagttgt ggcaattgac aggaacacta
      781 aacaagttct agctgtaggt gaggacgcaa agaaaatgat cggcaggacc cctgggaaca
      841 tagtggcgat tagaccattg aaggatggtg taatagctga ttttgatatt acacaggaga
      901 tgttgaagta tttcataaga aaggccaccc agagaaagtc tgtttttcaa cctagagttg
      961 tagtatgtgt accaagtggg gtaactgagg ttgagaagag agcagttgag gaagctacaa
     1021 tccaagcagg tgccaaggaa gcctttctta ttgaggagcc catggctgct gcaatcggtg
     1081 cgggacttcc tgtccatgat gcaacaggta gcatggtggt ggatatagga gggggtacca
     1141 ctgaggtagc ggtaatatct ctaggaggca tagttacaag caagtccatc agggtaggtg
     1201 gagatgaact tgatacctac attgtcaact atgtaaagaa ggaatatagc ttgatgatag
     1261 gtgaaagaac tgctgaagag gtaaaactcg gcatagggtc tgcaataaat gatcctgagc
     1321 tgggaacaat ggaaatcaga ggaagggacc tggtcagtgg cttgcctaag acgttggaaa
     1381 tatcttcaaa ggaaatatat gaggctctaa gggagccagt gaacaacatt attgactcga
     1441 tcaagtctac ccttgaaaag actccaccag agctatcatc agatgtcatg gagtttggaa
     1501 ttgtgcttac aggaggaggg gccatgctta acgggttgga ttccctgatc accaaagaaa
     1561 ctggaattcc tgtatttgtt gctgatgatc cgctggactg cgttgcaaac ggaaccggaa
     1621 aggcactgga tagtattgaa atattaaaga agacatcatc aagaaataat agattaaggt
     1681 agtgattcca tgcatttcct aagaaaatac aaggaaagaa tgatagtgat tgcaatcgct
     1741 atcattctgc ttattgtaat aggtcaaaca agtaaagaga gaatttcact gtcggggatt
     1801 gaaagattaa ccggtaatat tctgtctcca ttgacaagtg gagtgaccac tgcaagggaa
     1861 ggaatagccg gttttttttc ttcaattggc gaaacctttg aagcaaggga agagaatgaa
     1921 ctgcttaagg atgaaatcaa aaggctccaa tcagagaacc gaaacctctt ggacataata
     1981 gggaaaacag attacctaag aaatgagatg gagctggaaa gggcaactga gcttaccttg
     2041 ctgaagggca aggttgttgg gaaggagccc ggaaattggt ttagcaggtt taccattaat
     2101 ctcggatcaa aggatggagt tataaagggc gccactgttg tacagggagt tgaggttgaa
     2161 cagcagcttt atcaggaagg gattgtcggc agggtagtgg atgttggtga taattgggcc
     2221 aaggtaacca ctgtaattga tgagctaaac agtgtatcat tcaaaatcat aagaactcag
     2281 gatggagggg tcctctctgg tggcctggat gggcagctgg aaggttacct atttgacttt
     2341 gaagcggatg taatagttgg ggacaagcta tacacatcgg gcttgggtgg agtttacacc
     2401 caggacatat atattggaga ggtaactgaa atacggacaa gccaggaaga attgaccaag
     2461 accatagtgg tacaaccagc agtagacttc aggaagctct acaatgtatt tattatagta
     2521 aattgaatga gggatttaat tgaccattct gcttattacc atgtcaataa tacttaacat
     2581 aataatccaa gctactctga taccaaatat cggagttttt ggcgttgttc caaatacagc
     2641 cctggttctt gtagtgatga tagcacttaa gaaagggaga atatatggat ctgccttcgg
     2701 actgctggca ggtcttttac aggatgtgct cttttccata acaataggtg tcaatgcatt
     2761 catattgttc tttattggct actttgtagg atttgcatac aatagtctaa caagggagtc
     2821 tggaataaac cattttatct tcactgcatt gtcaacggta ttctacaatg tgcttttttc
     2881 gcttatacag ttcttccttt ccagggatgt tagctttgtt gatgccgtcc agagcatatt
     2941 ttcactggaa gtggtataca atggaatcat cgctgtgttg ttatttatgc ttattcaaag
     3001 gttattcaca cagccaaact taaagtttgg caggaggtaa gatgtaatga agcttagaga
     3061 gaaacttttc aacagataca gtttaatgat atattttctt gttctgatta caacgatgct
     3121 tctctttaga cttgctacct tgaccattgc acagggagac tattacaggg atctctcaga
     3181 caacaagagg gtcaaggagg tctataccac cgcaccaaga ggtgaaattc gagacagaca
     3241 cggtcgacta cttgcaggta atgttcctac attcactgtg cagctactta aggacgaatt
     3301 gaactttgga gatcgaactg taaggaatga cagtttgctt cagctggtga ggctacttga
     3361 gagcgatggg acagagtatg caaacgacta tcccgtagag cttaacctta tcaagtacag
     3421 gtcaatagat tcctatttga ccaatgaggt tgggccaatg gataagctgg taatccagat
     3481 tcttgaaaac aatctattga atgagttcct gaacatggaa ttttctaagc cctacaatga
     3541 ccattttggt ctttccatat tcaaaagggc gcaggatata ctcagcgaca agaatcctga
     3601 gatatattca ctggaccctg gcaatgaaga agatgtacta aagatctccc aactccttca
     3661 ggatgacaag gtgaacacca ggaaaatagt cgaccatcct gtggccagac tgctggcata
     3721 tgacctgctg agaaagtacg acctacagga ggaccttgcc cttgaagagt atgctttgac
     3781 ctactatgaa gattatattc agcaaaaggc agacctgatg gatgactatc catatatcac
     3841 aatggataca agtggtgagg aggattttat aaacctattc agtgaaatag gattatattc
     3901 attccttagc agggcaattc cattgggggg tgaaacagaa gaatacctgg tacctggaga
     3961 ggtgctgatt gagtatcttg aagaaaacgg aggaagctca ccaatagaaa tcaggatagg
     4021 cgacaatgga accgacctcc tgtacagctt tgtagacaca acaggaattt cagaccagga
     4081 cccgttggac cacctaattg gacttgctga ggaaatgaat ttgctggctg ggtttataaa
     4141 ggatgaaagg atcaggtacc aggctcagtc ccaccttatt aacagtggaa tcaatccgag
     4201 gatttccgtt gcgggtgata ttgagtatgt tcacataaac aatatgaaca actggtatga
     4261 agcgaataag gtagagaagg gaacacctct cgatgaagca ttcatggatg tcaggagaat
     4321 atatagcatc aatgaaagca tatcccctta tgaggcaagg gcgttgctgg gattgatcaa
     4381 ccagttgagc attcaaggat acctggcata ccagcccata aatatcgcat atggaataaa
     4441 ggatgagacc gtagcaagaa ttgaggaagg cttatctgga gttccaggga taaatgtttc
     4501 cattgagccc gtaagggtct atcctgaagg cacaacagca tcccatatac taggctatat
     4561 cgggaaaatc agtcagccct ttgaaattga aaagtacatc agggaagaag ggtattcgcc
     4621 agggacacta atcgggaaaa caggaattga ggagagttat gagcttcaat taagcggtgt
     4681 gagcggaatc aggaaggtgg aggttgacgt agttggcaat acaactaatg tcatagagga
     4741 gatcagtcct gtgcctggag acaatattta cctctccatt gacctcaagc tgcaaaagac
     4801 agctgaggaa gcaatgaaac ataccctgga ccagcttagc aaaggtggaa ccttcaaaag
     4861 tgaatgggga gattatacat ttgggaccca tcgcaggaag ggacgaccct atataaatgc
     4921 caattctggg gctgtggtgg tgcttgatgt aaagacaggt gaaactctgg ccatggttag
     4981 ctacccatcc tttgacccca atcttttctc cactggaatc aacaacacag actggttgag
     5041 tctgtttcca gaggatgccg ataacccctt ggctgcaaga ccattgtaca acatagccac
     5101 tcagacagcc gtacagcctg gttcaacctt caagatggtc acaggcttga ctgcccttga
     5161 gaagggtctt aatccccttc tgaggatcag ggacatgggt tatgtaactg tgggtgacaa
     5221 gaggtttcat tgcctaatat acaccaatac tgggggtaca cacggttatg agaatttcta
     5281 tgaggccttg agtgattcat gcaactacta tttctatacc ctggcccttg gaagaaacca
     5341 aaggacaggc caggacatcg gggtaaagat tgaaatagag gacattgtag aaatgtccaa
     5401 aaagcttggg cttaatgaca aaactggcat tgagataaac ataccatctg aggtttcagg
     5461 tggggtgcct gatccccagc gaaaaatcat caccactaaa tatcttttaa agcaaatgct
     5521 tcaaagaagt atggaaggtt acattatgga tggccatgaa ttcagtgagg aagatattca
     5581 gcttgccata gacgagataa taagctggac agaacttgaa gaaacactat caagagctga
     5641 agttgtaaga agacttaccg gcatgggtat cgaacctgag atgaggcttg ccggtgaacg
     5701 ggaaggcctt gctgataaga tcaagtacac ctacctcaac caggctggat ggaatatttc
     5761 agatacactg aacgtaacca tcggtcaggg acaaagtgca tacacaccta tacaaatggc
     5821 aaattacatc gctaccatag ccaacggagg ttatcttaac agggtaacac ttattgacag
     5881 tataagaaac tacaacaacg tttcagtcct atatgaacac aaacctgaga gagagctgat
     5941 tggcctgaat gactatgaaa accttgagca tgtgaagcaa gggatggaaa aggttgcaga
     6001 tgatggaacc gcaagaagga tattccaaaa ctttcctgtt cctgttgggg taaagacagg
     6061 tacagcagag agaagcggca ttaatccatc aactggcgat acctatgacg acttcgcttg
     6121 gtttgttggc tttgccccct atgatgaccc tgaaatagca gttgccgcag ttcttttcca
     6181 gggaggttca ggaggctacg ctgcacccat ggttagggag attatcgctg agtatcttgg
     6241 attaaacgat gttgagcaga ttgataccct gccaatggag aactccattg tatttgatta
     6301 gaacaaaata aaaatctaaa actatggatt tttacaccat aaactagtag aattagaata
     6361 aggcattagt ttggaggtag cacatgagaa aagatgtagt ttctatcaag ggagttgctg
     6421 gtggcattta cttaaatgtc gaaggaaatg atcttggaca gatcaagaca gagcttgaaa
     6481 gcaaaatgaa gacagcttca aatttcttta agggtagcaa gttcctggga gtaaggggag
     6541 aagaccttac agaggagaat agggttgaaa tcatgctgct tctaaagtat aaatacgatt
     6601 ttgagattca gattgaaaag gttcctgatc atgttgtaaa ggacaattat gaacctgtta
     6661 aggaaatgat tgcagctgaa ccggaagtag tacaggaagg catgaccaag ttcgtgcacg
     6721 gtaccctaag gtcaggacag gaggtgtcct acaacggaaa tattgtggta ataggcgatg
     6781 tgaaccctgg ggccctcttg aaggcaaagg gaaacatagt agtcctgggc tcattgaggg
     6841 gtgttgcaca agcaggtatc ggaggcaata ggcatgcagt tgtcgcagct tacagtttgc
     6901 tgcccactca gcttagaata gcagactata tagttaggcc cccagaggat gatatacagt
     6961 acaagtatcc tgaaatagca agagtggttg atggtgcagt tgtaatagaa ccttatttac
     7021 ctaataaata aaagggagga acataaatgg ctaaagataa gatgggaaca gctattgtta
     7081 ttacatccgg taaaggtggt gttggtaaga caacaacaag tgcaaacatc ggaaccgggt
     7141 tggcaatact cgggtattcg gttgtagtgc ttgatgcaga tattgggttg agaaacctgg
     7201 atgttgtaat gggacttgaa aataggatag tttacgatat tgtagacgta gttgaaaaga
     7261 actgcaagct taagcaggca ctgataaagg acaagagata cgagaagctg tacttactgc
     7321 cagctgccca gacaaaggat aaaaccgctg ttaagcctga gcagatggta gaactggtag
     7381 gtgaactgaa gaagacccac gactacatta taattgactc gcctgcggga attgaacagg
     7441 gattccagta ttcaatcgct ggagcagacc gaggaataat cgtaaccaca cctgagatat
     7501 cagctgtaag ggatgcagac agagtcattg gcattcttga agccaatgag cttcacgacc
     7561 cccaactgat aataaacaga atcagaccag acatggtcaa acgtggtgac atgatgaaca
     7621 tggatgacat tatcgatatt cttgcaattg atcttattgg gatagtacct gatgatgaat
     7681 caatagtaat ctcaacaaac aagggggagc ctgtagttat ggatgagaag gcattggcag
     7741 gcaaggcata cagaaatatc gttagaagaa tcaccggtga ggaagttgag ctgctgaatc
     7801 tagaggaaaa tgatgagagt aagttcagta aggttcttaa acttatattt ggcaagtaaa
     7861 ggaggcgaac ggtttggatt ttttcaaaat gtttactaga aaaaaggaaa acagtaaaaa
     7921 tgttgcaaaa gaaagattga aattggtatt ggttcatgat cgctccgacc tgtctcctag
     7981 atttcttgaa atgatcaagg gtgatattgt caaagtgatt tcacaatacg cagaaattga
     8041 tgagtcagaa atcgaaatca agatgaccaa gatgaagaag gaaggcaaca agtctcccgt
     8101 atcagcactg gttgcaaaca tcccaattgt taatgtaaaa gacaagaaga agtgattgct
     8161 ctaaccttgg ttagagcttt tctttgcctg tgggaaagat ttcccttgag gcagtaaatc
     8221 aattgaaagg tgaattgaat gattaataaa tttaagttgg ataaagtttt gatgaaggta
     8281 gaaaaacctt ccagatatat tggaatggag cagaacatgg tacagaaaaa cccggatgag
     8341 gttgatgtta gttttgcttt ttcatttcct gatgtctatg aggtaggaat gtcacaccta
     8401 ggaatgcata tcctctataa ccttataaac aatgaatcca gatactcatg tgaaagggtt
     8461 tttgcccctt gggtggacat ggaagcccag atgcgcaagg agtccatacc cctgtttaca
     8521 attgagtcca aggaggaagt caagaatttt gatttcctgg gattcactct tcagtatgaa
     8581 atgagctata ccaacattct gaatattttg gacctgtcag ggattccgat ccattccagg
     8641 gacaggggtg gtgagtttcc actggtcatt gccggtggcc cctgtgcata caatcccgag
     8701 ccattgtggg agttcattga cctctttgta ataggtgagg gcgaggaggt tacactggac
     8761 ctgatcaaac tctatgacaa gcataagaag aagggctatg acaagagaga atttcttgag
     8821 gaagcacagg ctacaatgga gggaatatat gtaccatccc tatacgatgt gaattacaat
     8881 gaggatggaa caatcaggga taggttccca tgggtctcag gattgcctga aacaatcagc
     8941 aagagaatga ttagggacct ggatagcatg ttttctccag aaaagatgct tgtaccgtat
     9001 atggagacag tccacgacag aatatcaatg gagttgttcc ggggatgtac acatggatgc
     9061 aggttctgtc aggcggggat gatatatagg ccaataaggg aaaaatccgt agacaggcta
     9121 atggagttgg cagaaaaact tgtggattca acaggctatg aaaacatttc attatcctct
     9181 ttgagctcat gcgactactc ccagctccaa cttttgatct caagcctgat ggataggttt
     9241 gaggataaaa aggtaggagt ctccctgcct tccttaaggt tggactctgt aagcatagac
     9301 attctgaagg aaattgagaa ggtgaggaag acaggcttga catttgcccc ggaggctggc
     9361 agccaaaggc ttagggatgt aatcaacaag ggtgttgacg aggaggacct tgtcaatgct
     9421 gtgggctatg cattcaagga aggttggtcg aggatcaagc tgtatttcat gataggcttg
     9481 ccaactgaga ccgaagacga tgtattgggt atcaaggagc ttgcatactt ggtaaaggac
     9541 ctgtttttca atagaccacc ggaagagcga aagggtaatt tttcagttac tgcaagtgca
     9601 tcctgctttg ttccaaagcc atttaccccc ttccaatggc atggacagga tacaattgag
     9661 gagtttcagg ccaagatcaa cctggtaaag gggagtatca aggataagaa ggtaaccttt
     9721 aattaccacg atcctagagt tagctatttg gaagccatag tggccagagg agaccggaaa
     9781 acaagcaagg tgatttacaa ggcctgggaa aagggatgta gatttgatgg ttggtctgaa
     9841 cactttaaat atgaaacctg gctggaagcc atggatgagc ttggaattga tggaagcttc
     9901 tatgcaaata gaaacaggga gttttcagaa aatcttccct gggatttcat caatccaggc
     9961 cccagcaaaa aatacctcca gaatgagtac accaaggcaa taaagggaga aacaacaagg
    10021 gattgccgac ttggttgcac cggttgcgga atacaggatt gtgccatgtg gggggtgttc
    10081 aattgatatt aaggacaagg tttaaaaaaa ttgggtattt gaagtacctt tcacatttgg
    10141 acttggttag gctgtttaca agaaccttct caaaggcagg aatacccatt aagttttcgg
    10201 aaggcttcaa cccccatcct aaattctcaa tagggaatcc tttgccattg ggtactgaga
    10261 gccttgcgga gtacatggat gttgagcttg ctgaggtgat caagcctgag atcttcatgg
    10321 aaaggatgaa caaggccttg cctgaaggaa tccagctagt ggactgtaga ttgattgaaa
    10381 agggtccttc ccttacttca atcatagcat ggtctcacta cgatataaga tttgtgtctg
    10441 aacaatcagg ggaggccatt gatgttgagg gagccctttc aagatggaac aaactctctg
    10501 aagtttatat aagcaagaag aaaaagaagg gtaagaagaa ggtggaaaga cagttggata
    10561 ttttgcccct gatcgggaat ttaaactaca agggcatgga cagtgatgga ttccacataa
    10621 ttagtggtct cttaaaatca ggagaatcgg ggaacttaaa accaatcgaa ctgattgaag
    10681 ccatggatag ggaacttgag cttaatattg atctggatat ggtaatgata aagcgtcttg
    10741 aagtatttgc tgatgaagat ggagagatca aggaacttgt ataggggtgc agcaaatgaa
    10801 cactatctat atcgattcaa gggatggagt caatagaata tgtattgtag aggacggcag
    10861 acttgtcgaa tactacaagg aggacacagg taggcacaga ttgcttggga atgtatacag
    10921 gggcagggtt gtgaatattc tccgggggat ggaggctgcc tttgttgaca tcggtgaaga
    10981 caggaacgcc tacctgaatg taaaggatgc ccttggcagg gagatgatgt actcaaagga
    11041 aaaattgacc attgaccaag tcctgaagca gggtgaggat ataattgttc aggttgtcaa
    11101 ggagccattg ggaaacaagg gacccaaggt caccacccac ctttcaatac ccggaaggta
    11161 catggtccta actccctatt ccaacagggt gaacatatca aaaaaaatca gagaccagga
    11221 tgagattgag agactgaggc atttgggtaa ggagattgaa agggaaagga tggggatgat
    11281 tttcagaacc gtttcccagg gggtggaatc agacctactt agggaggaat acaatgacct
    11341 ggtcagaaaa tttgaggcaa ttgaaggtga aagaaattat ctgcccacac caaaactgat
    11401 atatagcgaa ccggttctgg tcctgcagat actcagggat tatttcgatg aatccaagac
    11461 ggatgtggtc accaacagtc gggacatact ggaatatcta aaaaccacaa ccaagcttag
    11521 ggactacaac cttggagaca ggctgacata tgatccaaat tacaacatgg actaccacat
    11581 gaacatacaa atggacatga aggaggccct ttccagaatg gttcagctta agagtggtgg
    11641 gtatatagtc atagatgaga cggaagctct cactgcaatt gatgtgaata ccggcaaata
    11701 tgtgggaaac cttaccctaa gcgacaccat agtaaggacc aacatcgagg cagccgtgga
    11761 aattgcaagg caggtgagac ttagggatat tggtggcata atcataatag attttattga
    11821 tatgaaggtc caggataaca ttgacattgt ccttggaaag ttggataagg agtttagaaa
    11881 ggacaagaat aagccttacg tggtaggaat gaccaagctt aatcttgttg aagtggtcag
    11941 aaagagaaac agaccaaccc ttgacaagga gaccaccata gtatgtccaa cctgcaatgg
    12001 caggggtcga ataaggaaaa atgaagcttg acaataaggg tttaatttgt taaactattt
    12061 taggtgtaga gccgctcaat atgggtttta agcatagtca cctgatattg gcgagactag
    12121 gtaagaggag gtgcagtaaa tgtacgcaat tattgaaaca ggcggtaagc aatacaaggt
    12181 tcaggaaggc gatgtagttt tcgttgagaa actaaacgct gaagaaggag acgctgttaa
    12241 ttttgacaag gttcttttaa tgtcaactga ttcaggagtg actcctggaa agccattcgt
    12301 agaaggcgcg aaggttgagg gaactgtcct tgcacaaggt aaggcaaaga aaattgttgt
    12361 tttcaagtat aaggcaaaaa agaacatcag aaagaagcaa ggacatagac aaccatttac
    12421 taaggttcaa attaacaaga ttgtaggtta attatgatca aggtaataat cggaactgac
    12481 aatactggga acattgaatc ctttgagatg tcaggacatg ctgaatatgg accatacggt
    12541 aaggacatag tttgtgcagc tgcttcagtt ttgggataca cggctcttag agccatggtc
    12601 gaagtggcag gtgtcgacga aggaagcctt gactacaagg tggacgatga tatgggctat
    12661 atgaaggtta atctgggaac agaattggaa aacggtaatt tatccgatgt tcaggtcata
    12721 ttaaggacat atgaagtcgg aatcagatca atgatagaaa gttaccctaa atatatcact
    12781 cttgaatata ggggaggtgg aactcgtgtt taaattgaat ttacaattgt ttgcttctaa
    12841 aaaaggagta ggtagttcaa agaacggtag agacagtgag tctaaaagac tgggtgtcaa
    12901 aagaggagat ggacagtttg tattggcagg aaatatcttg gtaagacaaa gaggaactaa
    12961 aatccatcca ggtaacaacg tacaaatagg tggggacgat actttatttg ccacagttga
    13021 tggtgttgtt aaatatgaaa gaaaaggtcg cgacaagaaa caagttagcg tctatcctcg
    13081 agaagaatta gcgtaaattt taagcttacc gtctggtaag ctttaatttt tttgttgtgt
    13141 agcatatgtt ttcccagaca gtgttaaaga cactttttag ggtaacaaat aaaagatggt
    13201 ttatgaccgg attgtataaa taaagtgaat gaggatgatg gtaatgttta ttgatgttgc
    13261 gagaatatca gttaaggcag gaaaaggcgg aaacggaata gtagcgtgga gaagggaaaa
    13321 atacgagc
//
LOCUS       DASRQF010000281         4483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_342055, whole genome shotgun sequence.
ACCESSION   DASRQF010000281 DASRQF010000000
VERSION     DASRQF010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342055"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="VFD79_07575"
     CDS             <1..1177
                     /locus_tag="VFD79_07575"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZH93948.1"
                     /translation="SFYENEKNNGVSHFIEHMLFKGTQNRTAREIAEAMDDIGGQINA
                     FTSKEHTCFYGKVIDEHLPIAIDVLGDMICNSSFRESEIQKEKGVIIEEINMYKDFPE
                     DLVFEMLTEQTYKDISLAYPILGTNDTVNTFNRDIVLSYFKDKYSPDQMVVSVVGSFS
                     EHQVMELLNKELGTFNINGMGDKNLPSYEFEAGKSIVGLYKDIEQLNIAMGFQGPSSY
                     SDDIFPMMIVNNILGGTISSRLFQEIRENRGLVYTIESSLTSYNGAGMFSIYAGLTKD
                     NLLEVSRLIKLEVDQLKNRLISEKELEKSKEHLKGSYILSLEGTFSKMYEMGKNLLHS
                     KPIETPEIVLDHINNIGIVDIERVVEKYLLWDQMNLSYVGPVKDVNKLNKELVGIFK"
     gene            1193..1624
                     /gene="dut"
                     /locus_tag="VFD79_07580"
     CDS             1193..1624
                     /gene="dut"
                     /locus_tag="VFD79_07580"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HZH93949.1"
                     /translation="MNVKVVNMGSLSLPEYKTAGASGVDLMANLESTVVLKPMERFMV
                     PTGLFVEIPEGYEAQIRGRSGLAAKNGISLANGVGTIDSDYRGEIKVILINLGNEPFE
                     IKHGDRIAQMILAKYEKIEFTLVDSLEETDRGSGGFGHTGK"
     gene            1765..2589
                     /locus_tag="VFD79_07585"
     CDS             1765..2589
                     /locus_tag="VFD79_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HZH93950.1"
                     /translation="MNWIEAIVLGIFQGVAEFLPISSSGHLALLQHLFGITEGNLFFT
                     EMLHFGTLISIIIVYFEDVKKIIIEFLSLVGNLLTGKKSGDLTDHQRLGLLILAGSVP
                     TAIIGITFEDFFESLYTSLIPIGIALLFTGFLLWFAERKGSESKDIQKMTFIDSLIIG
                     TFQGMAIIPGISRSGSTIVAGLLRGLKKPLTTEFSFLLALPATFGAFLLGIIKVIRGG
                     NGVIVNMPLVVGVVLSTVVGIISLKALIKILNRNKLHYFSYYLWIVGAVTIISQLI"
     gene            2664..>4483
                     /locus_tag="VFD79_07590"
     CDS             2664..>4483
                     /locus_tag="VFD79_07590"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HZH93951.1"
                     /translation="MNTDILGMIILAVGVIVLISLFSSRMGIIGNVINGTTFSMMGFG
                     GYFFPLFIIGAGVVLLLERFDKIQLRIILSLSIIFIGFLIVLDGINPLDLTFLERIRQ
                     GVEISKANRGGGLLGSFFGFFLYKLFGSIGTYVVVAFAITVSLLIITNLTVKEVISKA
                     KKITPQMDAVTGKRRQKEVSKEDGKKNLKALTIDDEQIRISDYSSQFQAENQEEPQEK
                     TPKKKPIKLDEEIVQKEAVQIDSQVKEKQEQAQKIVYRIPPMSLLNSPGKKGESNDRK
                     HILDNARMIEETMKNFGIEASVSHINVGPTITCYELSPAPGIKLSRIVSLSDNIALSL
                     ASSDIRIEAPIPGKAAVGIEVPNKVKDMVLFKELMQSREFADTDTDIPLALGKDINGK
                     VVVSSIDKMPHLLIAGATGSGKSVCINTIIMSLVYKSSPDDVKLLLIDPKVVELSVYN
                     GIPHLLIPVVTDAKKAAFALNWAVGEMEKRYKLFAKNNVRDIKSYNEKHGEDGEKIPK
                     IVIIIDELADLMMVAAQEIEDYIARLAQMARAAGMYLIVATQRPSVDVITGTIKANIP
                     SRISFAVSSQVDSRTILDMAGAEKLLGQGDMLFYPSSYSKP"
BASE COUNT         1442 a          797 c         1065 g         1179 t
ORIGIN      
        1 atctttttat gagaatgaga aaaacaacgg ggtgtctcac tttatagagc acatgctttt
       61 caagggaacc caaaatagga cagcaaggga aattgctgaa gcaatggatg acatcggtgg
      121 gcagataaat gcattcacaa gcaaggaaca cacttgcttt tatggcaagg ttatcgatga
      181 acacctgcct atagccatag atgtcctggg agacatgata tgcaactcca gcttcaggga
      241 aagtgaaatt caaaaggaga agggggtaat aattgaggaa atcaatatgt ataaggattt
      301 tccggaggac ctggtatttg aaatgctaac ggagcaaacc tacaaggata taagcctcgc
      361 ctatccaatt ctaggtacaa atgacacggt gaacaccttc aatagagata ttgtactttc
      421 ctatttcaaa gataaataca gtcctgatca aatggtggtt tcagtggttg gtagcttttc
      481 cgaacaccag gttatggagc ttcttaacaa ggagcttggg accttcaaca taaatggcat
      541 gggggacaag aacctaccct catacgaatt tgaagctggc aagtcaattg tgggcttgta
      601 taaggatatt gagcagttga atattgccat gggatttcaa gggccatcaa gttactctga
      661 tgatattttc cctatgatga ttgtaaacaa tatattggga ggtacaatta gctccagact
      721 gtttcaggaa atcagggaaa acagaggatt ggtttacaca atagagtctt ccctcacgtc
      781 ttacaacggg gcgggaatgt tctcaattta tgctggtttg accaaggata acctactgga
      841 agtgtccagg ctgatcaagc ttgaagttga ccagctcaag aataggctca tatctgagaa
      901 agagctggag aaatcaaagg aacacttaaa gggcagctat atattgagtc ttgaaggcac
      961 cttcagcaaa atgtatgaaa tgggtaagaa cctattgcac agcaagccca tagagactcc
     1021 agaaatcgta ttggaccata taaacaatat tgggattgtg gacatagaac gagtggttga
     1081 aaagtacctt ttatgggatc agatgaatct atcctacgtg ggtccagtca aggacgtcaa
     1141 taaactcaat aaggaactgg tagggatatt taagtgatgg gaggaattta atatgaatgt
     1201 aaaggttgtc aacatgggaa gtctatcatt acctgagtac aagacagcag gagcatcagg
     1261 agttgatctg atggcaaacc ttgaaagtac tgtcgttcta aagcctatgg aaaggttcat
     1321 ggtccctacg ggcctctttg ttgaaattcc tgaaggctac gaagcccaga ttagaggcag
     1381 gagcggattg gcagcaaaga atggaattag tctggctaat ggtgtgggga ctatcgacag
     1441 tgattatagg ggtgaaatca aggtgattct aataaatctt ggaaatgagc cttttgaaat
     1501 caagcatgga gataggattg ctcaaatgat tcttgctaag tatgaaaaaa ttgaatttac
     1561 cctagtggat tctctggagg aaaccgatag aggtagtggg ggctttgggc atacaggtaa
     1621 ataatgtcta ctgccagcca cagcagcaag gtgatatgta actttaaatg tgttataatc
     1681 aagttatgca taaaatacat ttacactaat tcaactacgc agtagaaaat actggatagt
     1741 ttttacgcat gaggaggtca taaattgaat tggatagaag cgatagtact tggcatattt
     1801 cagggtgtag cagagttcct ccccataagt agttcaggac acttggccct actacaacat
     1861 ttgtttggaa taactgaagg gaacctgttc tttactgaaa tgttgcattt tggaacatta
     1921 atatcaatta tcatagttta ttttgaggat gtcaagaaaa taatcattga atttctcagt
     1981 ctggttggaa acctgctgac aggcaagaaa agtggagacc tgaccgacca tcaaagactt
     2041 ggtcttctga ttctggcagg ttctgtaccg acagcgatca ttggaataac cttcgaggac
     2101 tttttcgaat ccctgtacac ttccttgata cctattggaa ttgcattgct gtttacagga
     2161 ttcctattat ggtttgcaga aaggaaaggt tccgaaagca aggatatcca gaagatgaca
     2221 tttattgact cgctgataat tggtaccttt cagggtatgg ctattattcc ggggatctca
     2281 aggtcaggct ctacaatagt ggcgggactg ctcagaggcc tgaagaagcc acttacaact
     2341 gagttttcct tcctattggc actgccggcc acatttggag cgtttctatt ggggataatc
     2401 aaggttataa ggggtggcaa cggagtaata gtaaatatgc cacttgttgt tggagttgta
     2461 ctgtcaacag ttgtaggaat aatatcatta aaagcgctta taaaaattct aaatagaaac
     2521 aaactccatt acttttcata ctacctatgg attgttgggg ctgtaacaat aatcagtcag
     2581 ctaatatgac agaggtgaat cagatgaaaa aaaagacaac aaccaaaaac agcaaaaaaa
     2641 gcaaagggaa ggctcctagt aaaatgaaca ctgatatact ggggatgatt atcctggcag
     2701 tgggcgtaat agtgcttata agtctattca gttcaaggat gggcataatt ggcaatgtga
     2761 taaatgggac caccttttca atgatgggct ttggaggata cttctttcca ctatttataa
     2821 taggtgccgg ggtagtactg ctacttgaaa gatttgacaa gatacagctt aggataattt
     2881 tatcactgtc aataatcttc attggattct taatagtact tgatggtata aaccctcttg
     2941 accttacttt ccttgaacgg ataaggcagg gtgtcgaaat atcgaaagca aacagggggg
     3001 gaggattact tggaagcttt tttggattct tcctatataa attattcgga tccatcggta
     3061 catatgtggt agtggcgttt gcaattacag taagcttgtt gataatcacc aacctgactg
     3121 tgaaggaggt tatctcaaag gcaaagaaga tcacacctca aatggatgcc gttacgggca
     3181 agagaaggca aaaggaagta tctaaagaag atgggaagaa gaatctaaag gcactgacta
     3241 ttgatgatga gcaaataagg atatcagact attcctcaca gtttcaggct gagaaccagg
     3301 aagagccgca agagaaaaca cccaagaaaa agcccataaa gctggatgag gaaattgtac
     3361 aaaaagaggc tgtgcagatt gattctcagg tgaaggaaaa gcaggagcag gctcaaaaga
     3421 ttgtttaccg gataccacct atgtctctgt tgaactctcc tggcaagaag ggtgaatcca
     3481 acgaccggaa gcatatactg gacaatgccc gaatgattga ggaaaccatg aagaactttg
     3541 gaatagaggc ctcagtgtcc catataaacg taggtccaac cataacctgc tatgaactgt
     3601 ctccagcacc tggaataaag cttagtagga tagtctccct atcagataat attgccttga
     3661 gtctggcttc ttctgatata aggatagaag cacccatacc aggcaaggct gccgttggga
     3721 tagaggtacc caacaaggta aaggatatgg tgcttttcaa ggagcttatg caatccaggg
     3781 aatttgctga tacagatacg gacatacccc ttgcattggg aaaggatatc aatggcaagg
     3841 tggtggtgtc gtcaattgat aaaatgccac atcttcttat tgcaggtgca accggctcag
     3901 gtaaaagcgt atgcatcaac accataatca tgagtcttgt ctataagagt tcaccggatg
     3961 atgtgaaact attactaatc gacccaaagg ttgttgagtt gagcgtatat aatgggatcc
     4021 cccacttgct aatacctgtg gtcactgatg caaaaaaggc ggccttcgcc cttaactggg
     4081 cagtgggtga aatggaaaag agatacaagc tttttgccaa gaacaatgtt agggatatca
     4141 aatcatacaa cgaaaaacat ggagaagatg gagaaaaaat tccaaagatt gtaataataa
     4201 tagatgagct agctgacctg atgatggtgg cagcacagga aattgaagac tacattgcaa
     4261 gattggccca gatggcaagg gctgctggaa tgtaccttat agtggcgacc cagcgaccat
     4321 ctgtcgatgt aataaccggt acaatcaagg ccaatattcc atcaagaatt agctttgcgg
     4381 tctcctccca ggttgactca agaacaattc ttgatatggc aggggctgaa aagctacttg
     4441 gccaggggga tatgcttttc tatccttcaa gctattcaaa acc
//
LOCUS       DASRQF010000282         3185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_169123, whole genome shotgun sequence.
ACCESSION   DASRQF010000282 DASRQF010000000
VERSION     DASRQF010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169123"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1017
                     /gene="hprK"
                     /locus_tag="VFD79_07595"
     CDS             64..1017
                     /gene="hprK"
                     /locus_tag="VFD79_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HZH93952.1"
                     /translation="MDYVILKDLIKEMSLEEVYLSADVDRVKIYTTEISRPGLQMAGY
                     YEKFVPDRVQILGSAEWHFLRDMDAEKRYETLDKFLSYPVPVIVVARNHEVFPELLEL
                     AIKHGKTIVRGNKTTSKLINDLVINLGLSLAPTATVHGVMMDVFGIGVLITGKSGVGK
                     SETALDLITRGCRLVADDMVEIKRVEDTLRGTCPELIRYFLEIRGVGILDIERLYGVG
                     AVKQFDYIDMVVELEAWEKGKEYDRIGLEEESVEILGISLPKITIPVKPGRNIAMIVE
                     VAAKNNRQKRLGYNAAEELDRRVRRSIEDRRKAFDKRTELE"
     gene            1166..>3185
                     /gene="nifJ"
                     /locus_tag="VFD79_07600"
     CDS             1166..>3185
                     /gene="nifJ"
                     /locus_tag="VFD79_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HZH93953.1"
                     /translation="MGKTMKTMDGNTAAAYVSYAFTDVAAIYPITPSSNMAESVDEWS
                     ANGKKNVFGQEVLVTEMQSEAGAAGAVHGSLQAGALTTTYTASQGLLLMVPNMYKMAG
                     ELLPAVFHVSARALASHALSIFGDHQDVMAARQTGFALLASGSVQEVMDLGGVAHLAA
                     IKGRVPFLHFFDGFRTSHEIQKIEVMEYEDLEKLLDYDALNEFRKRGLNPENPVTRGT
                     AQNPDIYFQAAEASNPYYLAVPDLVNDYMKEINKITGRDYKPFNYYGDPEAENILVAM
                     GSVTETAEETVDYLMAKGEKVGLVKVHLYRPFSAKYFFDILPSTVKKIVVLDRTKEKG
                     ALGEPLYLDIKSLFYKKDNAPLIVGGRYGLGSKDTTPSQILAVYNNLKADQPKDGFTI
                     GIVDDVTNLSLPVETPIQTEPEGTIQCKFWGFGSDGTVGANKSAIKIIGDDTDMYAQG
                     YFAYDSKKSGGVTISHLRFGKSPIKSTYLISDADMISCSKQSYVHSYDLLKGLKKGGT
                     FLLNCLWDDEELETNLPASMKKYIAENEIKFYTINATKIAVDIGLGGRINMIMQSAFF
                     KLSEVLPLEEAVDHLKKSIVHDYGKKGEKIVNMNYEAVDKGINALHRVEVPASWKDAV
                     DEKTTSKDAPDFIKNVVEPMNRQEGDDLPVSTFVGREDGTFPMGTSEYE"
BASE COUNT         1024 a          609 c          783 g          769 t
ORIGIN      
        1 tatttccttg cagcggccat aattcccaga aggccgtatg atgccgaata gaggggtgtg
       61 gagatggatt acgttatact gaaggacctg atcaaggaaa tgagccttga ggaagtttac
      121 ctgtcagctg atgttgacag ggtgaaaatc tatacaacag aaataagcag accaggttta
      181 cagatggctg gatactatga aaaattcgta cctgacaggg tccagatatt gggaagtgcc
      241 gaatggcatt ttttaaggga tatggatgct gaaaaaaggt acgaaaccct ggataagttt
      301 ctaagttatc cggttccggt tattgtggtg gcaagaaacc atgaagtgtt tccagaacta
      361 ttggagcttg caataaaaca tgggaagacc atagtcaggg gcaataagac aacatccaaa
      421 ctgatcaacg acctggttat aaatctggga ctttcattgg cacctaccgc cacggtacac
      481 ggtgtcatga tggacgtgtt tggaatagga gtccttataa caggaaagtc aggagtggga
      541 aagtctgaga cggccctgga ccttataaca agaggctgca ggctggtggc tgatgacatg
      601 gttgaaataa agagggttga ggatacattg aggggaacat gcccggagct gataaggtat
      661 ttccttgaaa taaggggagt tggaatactg gacatagaga ggctgtacgg tgtgggagcg
      721 gtaaagcaat ttgactacat cgacatggtt gttgagctgg aagcctggga aaagggtaag
      781 gaatacgaca ggattggact tgaggaggaa agtgtggaga ttctggggat cagcctgccc
      841 aagattacaa tacctgttaa gcctggtagg aacattgcaa tgattgttga agttgcagcc
      901 aagaacaacc gtcagaaacg tcttggatac aatgcggccg aggagcttga ccggagggtc
      961 agacgttcaa tagaagaccg cagaaaagct tttgacaaga gaacagaatt ggaataattc
     1021 aagaattatg aagcaatatt gagttgactt tatttagggt ttttaatata ctaattatgg
     1081 taactaattt tttaacaatt tcacatattg ggcataagcc ctttttcgtt ttattacact
     1141 acactaacca aggaggtact attacatggg taaaactatg aagaccatgg atggtaacac
     1201 agctgcagca tatgtatcat atgcatttac tgatgtggct gcgatatatc caattacacc
     1261 atcatcaaac atggcggagt cagttgacga atggtcagca aacggtaaga aaaacgtatt
     1321 tggacaagag gtacttgtta ctgagatgca atcagaggca ggagcggcag gagctgttca
     1381 cggctcactg caggctggag cattaaccac tacatacaca gcttctcagg gacttctact
     1441 gatggttcca aacatgtaca aaatggcagg agagcttctt cctgcagtat tccacgtatc
     1501 agccagagct cttgcatcac acgcattgag tatctttgga gatcaccagg acgtaatggc
     1561 agcgagacaa actggttttg cgctgcttgc atcaggatca gttcaagagg ttatggacct
     1621 gggtggagtt gcccatcttg cagcaatcaa gggaagagtt ccattcctac acttctttga
     1681 tggattcaga acaagtcatg agattcagaa aatagaggtt atggagtacg aagaccttga
     1741 aaagcttctt gactacgatg cccttaatga gttcagaaag aggggactta atccagagaa
     1801 tccagtaacc agaggaactg cccagaaccc tgacatctac ttccaggcag cagaggcttc
     1861 aaatccatac tacctggctg ttccagacct ggtaaacgac tacatgaagg aaatcaacaa
     1921 gataacagga agggactaca agcctttcaa ctattatgga gaccctgaag cggaaaatat
     1981 ccttgtagct atgggatcag ttaccgaaac tgctgaagag actgttgact accttatggc
     2041 taaaggagag aaggttggac tggttaaggt tcacctatac agacctttct cagccaaata
     2101 cttctttgac atattgccat caacagtcaa gaagattgta gttcttgaca gaaccaagga
     2161 aaagggagct cttggagagc cactatacct tgatatcaag agcctgttct ataagaagga
     2221 caatgcacct ctaatagttg gaggaagata tggtcttgga tcaaaggata caacaccatc
     2281 acagattctt gcagtttaca acaacctaaa ggcagaccag cctaaggatg gattcacaat
     2341 aggaatcgtt gatgatgtaa caaatctttc acttccagtt gagacaccaa tccaaactga
     2401 acctgaagga acaatccagt gtaaattctg gggattcgga tcagacggaa ctgttggagc
     2461 caacaagtca gccatcaaga tcataggtga tgacactgac atgtatgcac agggatactt
     2521 tgcatatgac tccaagaaat ctggtggagt tacaatatca caccttagat ttggaaagtc
     2581 tccaataaag tcgacatacc ttatttcaga tgcagacatg atctcatgct caaagcagtc
     2641 atacgtacac agctatgacc tgctaaaagg acttaagaag ggtggaacat tcctgcttaa
     2701 ctgcctgtgg gatgatgagg agcttgaaac aaaccttcct gcatcaatga agaaatacat
     2761 tgcagaaaac gagattaagt tctacacaat aaatgcaaca aagatcgccg tagacatcgg
     2821 ccttggcgga agaataaaca tgataatgca gtctgcattc ttcaagcttt cagaggttct
     2881 tcctcttgag gaagctgtag accacctaaa gaaatccata gttcacgact atggcaagaa
     2941 gggtgagaag attgtaaaca tgaactatga ggctgtagac aagggaatca atgcccttca
     3001 cagggttgaa gtacctgctt catggaagga tgcagtggat gagaagacta catccaagga
     3061 tgcacctgac tttatcaaga acgttgttga gcctatgaac agacaagagg gagatgacct
     3121 tccagttagc acattcgtag gtagagaaga cggaacattc ccaatgggaa cttcagaata
     3181 cgaga
//
LOCUS       DASRQF010000283         4103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_664399, whole genome shotgun sequence.
ACCESSION   DASRQF010000283 DASRQF010000000
VERSION     DASRQF010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664399"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..943)
                     /locus_tag="VFD79_07605"
     CDS             complement(2..943)
                     /locus_tag="VFD79_07605"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZH93954.1"
                     /translation="MDNSKALAVLANLRKAIKGKDDVLEIVVMALLAQGHLLIEDVPG
                     VGKTTLVKALAKSMNLEYRRIQFTPDLMPSDIIGVNIFQKETSSFVFRKGPIFTQILL
                     ADEINRTSPKTQSSLLQAMEEGEVSTEDRDYVLRKPFMVLATQNPLEYQGTFPLPEAQ
                     LDRFLMRISLGYPSKEDEIEILHNYQGIRNLVDVSPVVTGEEILDMQESVASLKVHDD
                     ILSYIVRISEVSRSYEDLRLGASPRASIDLLKASKACAFIKGRDYVIPDDVKEVAIPV
                     LGHRMILSSQARIERKSIESILKGVLKRVTVPVLSHE"
     gene            1145..3373
                     /locus_tag="VFD79_07610"
     CDS             1145..3373
                     /locus_tag="VFD79_07610"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HZH93955.1"
                     /translation="MRKFVSALLIMVMTIGGSLSFGVEARGNDISLHLGSPLILSGSD
                     MIPLDSENPGVVPVIHNDRTLVPLRAISEHFGAEVDFDPVKREASIMFEGVQYLFPID
                     KNYYDVLGGEEAATRVEFDTETLIIQDRTMVPLRIIAESILEMDVAYDDRVIFIGKGQ
                     DELNDELVLEVKNKIGQALKVGSREELLALIDNMEEAPMEEAVREPVMENQAPAMDGS
                     AGARDDSKDFSITNEQVEGINEADIVKTDGTYIYVATRDSVKIYRANDGRPVLTDSME
                     VMLDKETGEYIEFTELYLDEGKLVVLGRKGAMGNWIRPMPEPMPMEDVMVDSIMPFYG
                     EEYVYLGVYEIDQEGKMNLVKELELEGGLLSSRKKDEHVYLVVNKYLSYYGPYPDKPI
                     PLFRDSTIGTEYREIPVDSILYHPGRRMSNYLLVAAVDIMDEKESANVEAYLGSGSEV
                     YMSENALYVSGNDYNSFWGTVTNIARFTVDGTRIAYSGGGMVQGSLLNQFSMDEYDGN
                     LRVATTEWRGDSLNSVYILDRNMDQIGSIENLAPGERIFSARFMGDIGYVVTFRQVDP
                     LFVLDLSDPENPKVTGELKVPGFSNYLHPISQDILLGIGQDVDEETGIQQGIKLSLFD
                     VSDSGKPREIKNLTLGDTGSYAEVLHNHKALMLKPEEELIAFDATLNTYSGKLAREAF
                     NGLVVVKASKAGDLQLVAEISNEGIYGQNVKRPVYIGDILYYVMDDQIRAFHMDDFEE
                     IK"
     gene            complement(3426..>4103)
                     /locus_tag="VFD79_07615"
     CDS             complement(3426..>4103)
                     /locus_tag="VFD79_07615"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93956.1"
                     /translation="FLGIFNEMNPYSPGLVTIFAVIGGIVLAAFLSGSAYLFASVKTK
                     LPYERFKTIMAGIFTALVPLAISILILYITEAGTIYLIGFAIGVVVIYLSPLAHRRTA
                     QGIKWMQHLLGLKNFIEKAERDRIKQLVDEDPEYFYHILPYAMVLGVTDQWAKNFEGI
                     AMRNPDWYQTNSTRAFHAPYFVSRMDANTRAVGKTMASSPAKSGGGSFGGGSSGGGSG
                     GGGGGGW"
BASE COUNT         1198 a          849 c          965 g         1091 t
ORIGIN      
        1 tctattcatg gcttaacacc ggaactgtca cccgctttaa aactcccttc agtattgatt
       61 ctatactttt tctttcgatt ctagcctggg atgataatat catacggtgg ccaagaacag
      121 gaatcgccac ttcctttaca tcatcgggaa tgacatagtc cctgcccttg ataaatgcac
      181 aagcctttga agccttcaac agatctatgg atgcccgtgg acttgctccc agtctcaggt
      241 cttcataaga cctgctcacc tctgaaatcc ttacgatata ggataggata tcatcatgga
      301 ccttaagaga ggctacactt tcctgcatat ccaatatttc ctctccagta accactggac
      361 ttacatccac gagattcctg attccttgat agttatgaag gatttcaatt tcgtcctcct
      421 tggagggata accaagggaa atccgcataa gaaacctatc aagttgtgct tcgggaagag
      481 ggaaggttcc ctggtattcc agagggtttt gggttgccag gaccataaag ggctttctca
      541 aaacataatc cctatcctct gtggatacct caccctcctc catagcctgc aacaggctgg
      601 attgtgtctt tggtgaggtc ctgtttatct catccgccag aagtatctgt gtgaaaatcg
      661 ggcccttcct aaacacaaag gatgaagttt ccttctgaaa aatattaacg ccgattatat
      721 ccgagggcat aaggtctgga gtgaattgaa tccttctata ttcaagattc atgctcttgg
      781 ccaaggcctt caccagggtg gtttttccta caccaggtac atcttctatg agcaggtggc
      841 cttgagccag aagggccatc acaactattt caagtacatc gtccttaccc tttattgcct
      901 tccttaaatt cgccagaaca gctaatgctt tagaattatc catgatttcc tcccaacaag
      961 tgtcttcatg atatttatac ctaaaaacaa atcatgtaca catgcaaagg gaaacctttt
     1021 acagagttta aagcaacaca cctataagta atacaatcgt aaccaaatag taaacacttc
     1081 acagtgatac atgggtataa tatccaatag gaagtacaat cggaccaaag ggaggtaaat
     1141 tgtaatgagg aagtttgtta gtgctttatt gatcatggtc atgaccattg gaggttcact
     1201 ttccttcgga gttgaagccc ggggaaatga tatttccctc catctgggaa gtccattgat
     1261 cctatcagga agtgacatga ttccccttga ttcagagaat ccaggagtgg tacctgttat
     1321 tcacaatgat aggacactgg tgcctttaag ggccatcagc gagcacttcg gcgctgaggt
     1381 tgattttgac ccggttaaga gagaggcttc aataatgttt gagggtgtgc agtacctgtt
     1441 cccgattgac aaaaactact acgatgtctt agggggtgag gaagctgcca cgagggttga
     1501 attcgacaca gaaacactga taatacagga cagaaccatg gtcccactga ggattattgc
     1561 agaaagtatt cttgaaatgg atgttgccta tgacgacaga gttatcttta ttggaaaggg
     1621 ccaggatgaa ttaaatgatg agttggttct tgaagtaaag aacaagattg gtcaggcatt
     1681 gaaggttgga agtagagaag agcttctggc actgattgat aacatggaag aagctcctat
     1741 ggaagaggcg gtgagggagc ctgtaatgga aaaccaggcg cctgcaatgg atggaagtgc
     1801 aggtgcacgg gatgactcca aagatttctc aattacaaac gagcaggttg aaggtataaa
     1861 tgaagctgac atcgtcaaga ctgatggaac ctatatttat gttgcaacca gggactcagt
     1921 aaaaatatac agggcaaatg atgggcgccc agtattgact gattccatgg aggttatgct
     1981 ggacaaggag acaggagaat acattgagtt caccgagtta tatttggatg aaggcaagct
     2041 tgttgtactg gggagaaagg gtgcaatggg aaactggatc agacccatgc ctgaaccaat
     2101 gccaatggag gatgtcatgg tagactccat catgccattt tatggtgagg agtatgtcta
     2161 tcttggagtg tatgaaattg atcaagaggg taaaatgaac ctggtcaagg agcttgagct
     2221 tgagggagga cttctttcca gcagaaagaa ggatgaacac gtataccttg tagtgaataa
     2281 atatctgtcc tattacggac catacccaga caagccaata ccgcttttca gggactccac
     2341 aattggcact gaatacaggg agatacctgt tgacagcata ctctaccatc caggaagaag
     2401 aatgtccaat tacctccttg tggcggcagt ggatataatg gatgagaagg aatcggccaa
     2461 tgttgaagct tacctgggta gtggaagtga ggtttacatg agtgaaaacg cactctatgt
     2521 gtcgggaaat gactacaact ccttctgggg tacagttaca aacattgcaa gattcacagt
     2581 tgatggaaca aggattgcct attcaggtgg aggaatggtt caagggagct tgctgaacca
     2641 gttttcaatg gatgagtatg atggaaatct aagggtggca acaacagaat ggaggggaga
     2701 tagtctaaat tcagtttata tacttgatag gaacatggac cagatcggtt ccattgagaa
     2761 ccttgcacct ggtgaaagga tcttttcggc aagatttatg ggggatattg gatacgtcgt
     2821 aacatttaga caggttgatc cgctttttgt gctggacctt tcagatccag agaatcccaa
     2881 ggttactgga gagcttaaag tccctggttt ctcaaactat ctccacccta tcagtcaaga
     2941 cattctgcta ggaataggac aggatgtgga tgaagaaaca ggaatacagc aaggcatcaa
     3001 gctttcattg tttgatgttt cagattcagg taaaccacga gaaatcaaga atcttactct
     3061 gggagatacc ggaagctatg cggaggttct ccataatcac aaggccttga tgcttaagcc
     3121 tgaggaagaa ctgatagcat ttgatgccac acttaacaca tattctggaa agcttgccag
     3181 ggaagccttc aacggcttgg tggtagtaaa ggcaagtaaa gcaggagacc tacagctggt
     3241 cgcagaaata tcaaatgaag gcatttatgg acaaaatgtt aagagaccgg tctatatcgg
     3301 tgacatcctt tactatgtta tggatgacca aataagggca ttccatatgg atgattttga
     3361 agaaataaaa tagcataaac aaggcctctg gaaatataat atccagaggc cttgcatttt
     3421 tttgattacc agccgccgcc accgccgccg cctgatccac cgccagagct tcctccacca
     3481 aagctccctc cccctgattt tgcaggactt gaggccattg tttttccaac agcccttgta
     3541 tttgcatcca tcctgctgac aaagtaaggg gcatggaagg ctctagttga attggtttgg
     3601 taccagtcgg gatttctcat tgcaattcct tcaaagttct ttgcccattg gtcagttaca
     3661 cccagtacca tcgcataggg taggatatgg tagaaatact cagggtcctc atccacaagc
     3721 tgcttgattc tatccctttc agccttctcg atgaaatttt tcaaaccaag caggtgctgc
     3781 atccacttga ttccctgggc tgttcttctg tgggccaggg gtgagaggta aattacaacc
     3841 acaccgattg caaagccaat tagatatatg gtgcccgcct cggtgatgta caggatcaga
     3901 attgatattg ccaggggtac aagagctgtg aatattccag ccatgatggt cttgaacctt
     3961 tcatagggaa gctttgtctt cacactggca aagaggtaag ctgatcctga taggaaggct
     4021 gccaaaacga taccgccaat tactgcgaaa atagtcacaa gacctggact gtaaggattc
     4081 atttcattga atataccaag aaa
//
LOCUS       DASRQF010000284         2462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_56520, whole genome shotgun sequence.
ACCESSION   DASRQF010000284 DASRQF010000000
VERSION     DASRQF010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56520"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="VFD79_07620"
     CDS             complement(<1..175)
                     /locus_tag="VFD79_07620"
                     /inference="COORDINATES: protein motif:HMM:NF018816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HZH93957.1"
                     /translation="MSKTNSIVTYGVFMALTVVMTMIIQIPAPASVGYLNLGDMVIFM
                     AAFMFGKKGGFLVG"
     gene            complement(293..1345)
                     /gene="corA"
                     /locus_tag="VFD79_07625"
     CDS             complement(293..1345)
                     /gene="corA"
                     /locus_tag="VFD79_07625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HZH93958.1"
                     /translation="MTREIKNIGKTVNLPPGTLQYTGQGEGDQLIILTRYTEKDIASE
                     RLTSLEGFPIVEHDSTVWLDVIGLRKPETVREISDLFNIHPLVQEDILNVSQIPKVEE
                     YDGYIFTVTKNVFFNEEGQLDTEQISIILINNMVITFQEYDTDSFKHIHERLKDSLSL
                     RKHGALELFYSLIDSVVDNYFLLLENIGDEIDSVEDRLLENPNKDILGSIYHLKRELV
                     YIRNILWPTRNLTSSLARGEFVEIDDRIALYFRDIYDHVIQMIDIVETYRDICSGMLD
                     TYLSSVGYKTNEVMKILTIFSTIFIPLTFLAGVYGMNFIYFPELDWKYGYLIFWLISG
                     IITAFMIRFLRKKGWL"
     gene            complement(1416..>2462)
                     /locus_tag="VFD79_07630"
     CDS             complement(1416..>2462)
                     /locus_tag="VFD79_07630"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZH93959.1"
                     /translation="LRNQIVGIMGAGPDISSLAATYLGTISIGITFLSMTIFISHNMI
                     ALGNPRISIISTSIGAIINIVLDFILVQGMGMGVQGAAMATAFSQIAGFTYAFYHYTK
                     MRKSYNIPLKFKLDSKLIAPILLVGVSAFITEAEDGILMGVLSSLLTSTAGDQGIIVL
                     GVVSKLYMFLFITIFGIASAMQPIAAYNVGARNFSRLRSLMNKTIVYAGLTSFVMWAV
                     AMLFTPQLIGIFIEDRAIIGEATKAFRTMIAVFPVISIYYVSIFYFQASGRAKMSISV
                     AVLRQLVIMLPVSLILVKGFNMGAAGVWLSYPIADLLSSIASFMLIRNEGIELNTAIA
                     AQKRAKTAKQAAVH"
BASE COUNT          690 a          560 c          456 g          756 t
ORIGIN      
        1 ctccaaccag gaaacctccc tttttaccaa acatgaatgc cgccataaaa attaccatat
       61 ctccaagatt caggtatccg acacttgcag gtgccggtat ttgtattatc atggtcatta
      121 caactgtaag ggccataaaa acaccatagg ttacaatgct gtttgttttg gacatctcta
      181 caccccattt gctttaattg taccgctaca ctattttgta tatagctact gtatctatct
      241 ttataataac acttaatttt taattttaca agccccctca aaaaaaaaag agctaaagcc
      301 agcccttttt ccttagaaac cttatcataa atgcagttat aatcccggag ataagccaaa
      361 atatgaggta tccatacttc cagtcaagct caggaaagta gatgaaattc attccataca
      421 cccctgccag aaatgtaaga gggatgaata ttgttgaaaa aattgtaagt atcttcataa
      481 cctcatttgt cttgtagcca acgcttgaaa ggtatgtatc cagcattccc gagcatatgt
      541 ctcggtaggt ctcaacgata tctatcatct ggatgacatg gtcgtaaatg tccctgaaat
      601 acaaggcaat cctatcgtct atctcaacaa actctcctct tgcaagggaa ctggtcaagt
      661 tccttgttgg ccagagtatg tttctgatat ataccagttc tctttttaga tggtatatgc
      721 tcccaagaat atccttgttg gggttttcca acagtctgtc ctcaactgag tcaatttcat
      781 cacctatgtt ttccaggagg aggaagtaat tgtccaccac agagtcaatg aggctgtaga
      841 acagctcaag tgcaccatgc tttctcaggg acaaggaatc ctttagtctt tcatgtatgt
      901 gtttgaagct gtctgtgtcg tattcctgaa atgttataac catattattg atcaatataa
      961 ttgaaatctg ctctgtgtcc agttgaccct cctcattgaa aaagacattc tttgttactg
     1021 tgaaaatgta gccgtcgtac tcctcaacct ttggaatctg ggaaacattt agtatgtcct
     1081 cctgtaccag gggatggata ttaaaaagat cgcttatttc cctaactgtt tcaggcttcc
     1141 taagaccgat cacatccaac caaaccgtac tgtcatgctc cactattgga aacccttcca
     1201 atgatgtcaa tctttcgctg gcaatgtcct tttctgtgta tcgggtcagt attatcaatt
     1261 ggtccccttc accttgtccg gtgtattgga gggttccagg tggcagattg acagttttac
     1321 cgatattctt aatttctctg gtcatatcat cgcctccagg gcataaaaaa acaggtagat
     1381 ttctctacct atttataccc aattattcat ttaaatcagt gtacagctgc ctgctttgct
     1441 gtttttgccc ttttttgggc tgcaattgct gtatttaact caattccttc attacgtatc
     1501 agcataaatg atgctatact ggacaatagg tcggctattg gatacgaaag ccatacccct
     1561 gcagccccca tattaaagcc cttaaccaaa atcagggata ctggaagcat gattaccagt
     1621 tgccttagaa cagccacgga aatggacatc ttggcccttc cacttgcctg gaagtagaag
     1681 attgacacat agtatatgct tattaccggg aatactgcta tcattgttcg gaaggccttg
     1741 gtggcttcgc caatgattgc cctatcctca atgaatatcc caattagctg tggtgtgaac
     1801 aacattgcaa ccgcccacat gacaaaggat gttagccctg cgtacactat ggtcttgttc
     1861 ataagggatc ttaaccttga gaagtttctc gctcccacat tgtatgccgc tattggctgc
     1921 atggcagatg ctattccaaa tatggttatg aaaaggaaca tatagagctt ggatacgact
     1981 ccaagaacga tgattccctg atctcctgcc gtacttgtga gaaggctgct cagaactccc
     2041 atgaggatcc catcctcagc ctctgttata aaggccgaca cccccacaag gaggattggc
     2101 gctatgagct tagaatcaag cttgaatttc aaaggaatat tgtagctttt tctcatcttt
     2161 gtatagtggt agaaggcata tgtgaatcct gcaatctgtg agaaagccgt tgccatggct
     2221 gctccctgaa ctcccattcc cataccctgt acaagaatga aatccaatac tatatttata
     2281 atggccccaa tgcttgtgct tataattgat attcttggat ttccaagtgc tatcatattg
     2341 tgggaaataa atattgtcat gcttaaaaat gtaatgccaa tgctaattgt tccaagatat
     2401 gtagcagcca gactactaat gtcaggaccc gctcccataa ttccgactat ctgattccta
     2461 ag
//
LOCUS       DASRQF010000285         3813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_664539, whole genome shotgun sequence.
ACCESSION   DASRQF010000285 DASRQF010000000
VERSION     DASRQF010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664539"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="VFD79_07635"
     CDS             complement(<1..715)
                     /locus_tag="VFD79_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZH93960.1"
                     /translation="MKKTFYLTTPIYYPSDNLHIGHTYTTVAADSIKRYKKLQGYDVF
                     FTTGSDEHGQKIQEKAQENNMTPKAYVDKIVADIKDLWKMLEIDYDSFIRSTDDFHEK
                     AIADIFTKLYEKGEIYKDEYEGAYCTPCESFWSESQLVDGNKCPDCGRETHLEKEEAY
                     FFKLSKYRDKLLKLYEDNPGFIQPESRKNEMINNFLKDGLDDLSVSRSSFDWGIKVPF
                     DDKHVVYVWIDALSCYLTAL"
     misc_binding    complement(768..994)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1031..1474)
                     /locus_tag="VFD79_07640"
     CDS             complement(1031..1474)
                     /locus_tag="VFD79_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005955681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HZH93961.1"
                     /translation="MKNRYIFPAIIKVEDDGYNVSFPDIEECFTFGEDLEDALDSARD
                     VLELCLYDMEKDGRAIPEASAIGDIDLERNETIAWINAWMIPVRDKMENKAVKKTLTI
                     PKWLNDIGVENNLNFSSLLQSAIKEKVGIYDANSKNRDLSPKKLD"
     gene            complement(1518..1718)
                     /locus_tag="VFD79_07645"
     CDS             complement(1518..1718)
                     /locus_tag="VFD79_07645"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HZH93962.1"
                     /translation="MEVPVGYTYKDVIKKIKEEGWSLKRTSGSHEIYKHPDGRTCPVK
                     CTSKEIPKGTMKSIERITGIKF"
     gene            2004..3029
                     /locus_tag="VFD79_07650"
     CDS             2004..3029
                     /locus_tag="VFD79_07650"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HZH93963.1"
                     /translation="MDNTKANVAKKVVVLLLLAIVMTISLGVSSYQGAEITLTVEGET
                     IRMVSYSSTVEELLMDEGIEFDKDTLVKPDLDTLLTPGLKITVTDPKTYTIVFKDETT
                     ELTSYKTRVEDILEEADIHVGPKDFSEPALNSEVKTNTTIEFFQVEEKLRVENTSIPF
                     DTEVINNIRLDKGISNTLQEGKDGLKRSHIKDIYINGEMSSSVVILDTVASEPLSQVI
                     ERGTNDLVSTSRGDVRFREAKILNASAYDLSYASTGKRPGDPGYGITASGTTARPGTV
                     AVDPRVIPLGTKLYVESLDGSRDYGFAIAEDTGGAIKGNKIDLFYHSNAEAMRFGRRN
                     VKVYILD"
     gene            complement(3062..>3813)
                     /locus_tag="VFD79_07655"
     CDS             complement(3062..>3813)
                     /locus_tag="VFD79_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93964.1"
                     /translation="EKGFAQGNLDQKRATETGIAGSIFFDESRIGFASMARETINYAN
                     SHDNLILQDKLLKVFPNKPRKDLVEYNKFIHAILILSQGVPFIHAGNEFMRTKDGREN
                     TYNAPMSINGMDWSLKEENLDLFKYIKDLIRLRKERQEFRMDQADEIRKKLRFIDEGA
                     TCPVIAYTIRGNGGYLLVIHNANQTSCLIPHSVIKKHIQAQDERRVRDLSIRCIFNQE
                     GFTSDEGEFLHPHGIENHPVSTKVFELKIKD"
BASE COUNT         1001 a          848 c          766 g         1198 t
ORIGIN      
        1 caagggctgt aaggtagcat gaaagggcat ctatccatac atataccacg tgcttgtcgt
       61 caaagggaac cttgattccc cagtcaaagc tgcttctgga tacgctcagg tcatccagac
      121 cgtccttgag gaagttgttg atcatttcat tcttcctgct ttccggctgg ataaagcctg
      181 gattgtcctc atatagctta aggagcttgt ccctgtattt tgaaagcttg aagaagtagg
      241 cctcctcctt ttcaaggtgg gtctccctgc cacagtcagg acacttgttg ccatccacaa
      301 gctgtgactc agaccagaag gattcacaag gtgtgcagta ggctccctcg tattcgtcct
      361 tgtagatctc tcccttttca tagagcttgg tgaagatgtc agctatggcc ttttcgtgga
      421 aatcatcggt gcttcttatg aatgaatcgt agtcgatctc aagcatcttc cacaggtcct
      481 tgatgtcggc aacaatcttg tcaacatatg ccttgggagt catgttgttc tcctgggcct
      541 tctcctggat cttctggccg tgctcgtcag agcctgttgt aaagaatacg tcgtatccct
      601 gaagcttctt atatctcttt atactgtctg ctgcaactgt tgtgtaggta tgtccgatat
      661 gaagattgtc gctgggatag taaatgggtg tggtcaaata aaacgtcttt ttcatctacc
      721 tcttcctctc cttaatgtat ttaaaattat aaaaagcctc catctcatat agagacgaag
      781 gctacttcgc ggtaccactc taattgtcag catctcacaa tgctgacctc agcaggttat
      841 aaaccctggc actttaacga ctgccgccgt cccggcctac atatcggccg gaaaattcag
      901 ggaccatctt cgacaggctt cctttcaccg gttttccacc atccccggct ctcttaagaa
      961 agtagacccc tactcttccc ttcattatcc ttaaatctcg attactaata attatagcct
     1021 atttggggaa ttagtcaagt tttttgggag ataagtccct atttttactg tttgcatcgt
     1081 atattccaac cttttccttt atggcactct gtagaaggga tgaaaagttc aaattgtttt
     1141 caacaccaat atcattaagc catttaggta tggtcagggt cttctttaca gctttgtttt
     1201 ccatcttgtc tcttacggga atcatccaag cattgatcca tgcaatggtt tcatttcttt
     1261 ccaggtcaat atctccaata gcagatgctt caggtattgc cctcccatcc ttttccatat
     1321 cgtataaaca cagctccaga acatcccttg cgctgtccaa tgcatcctcc agatcctctc
     1381 caaatgtgaa gcattcttca atatccggga agctcacatt gtagccatcg tcctccacct
     1441 taattatagc aggaaaaata tatctgttct tcatggtctc tcctccattt ctaccaacta
     1501 cacatttgct ttttatacta gaatttaatc cctgttattc tttcaatact tttcattgtt
     1561 cctttaggaa tctccttgct tgtacattta actggacatg ttcttccatc tggatgcttg
     1621 tatatttcat gtgaaccact ggttcttttt aatgaccagc cttcttcttt tatcttcttg
     1681 attacatcct tgtatgtata tcccacagga acctccatat tctaaaatta tttatatatg
     1741 aattgctatt ctctatggtt attatatacg tattattaac acgtgtcaat attttcttgt
     1801 gacttttact gttataacaa taacattatc ttatccgttg caatttcaat gattacacta
     1861 tgtttagctt ttggttaatt atcagaatat tccggatata tgggcaaaaa tttgacaaag
     1921 tatatttttt ggtttattat acatggagca tcacaaatag ttacaaattg gttacaatct
     1981 gtaacaaata aaggaggaga attatggaca ataccaaagc caatgtggct aaaaaggtag
     2041 tggtcctctt acttctggct atagtaatga caatatcatt gggagttagt tcctaccagg
     2101 gggcagaaat aacactcaca gtggaaggtg agacaataag gatggtctca tattcatcaa
     2161 cagttgagga acttctaatg gacgagggga ttgagtttga caaggataca cttgttaagc
     2221 ctgatctgga cacactttta acaccaggtt taaagattac cgtaacagac ccaaagacat
     2281 acaccattgt tttcaaggat gaaacgactg agcttacatc ctacaagaca agggttgagg
     2341 acattcttga agaggcagac atacatgttg ggccaaagga cttttcagaa ccggccctga
     2401 attcagaagt aaagacaaat accacaatag agtttttcca ggttgaggaa aagctgagag
     2461 tggagaacac ctctattcca tttgatacag aggtaataaa taacataaga ctggacaagg
     2521 gtatttcaaa tactctccag gaaggaaaag acggattaaa aagatcgcac attaaggaca
     2581 tatatattaa tggtgaaatg tcttcgtctg tcgtaatcct tgataccgta gcgtcggaac
     2641 ctctttctca ggtaatcgaa aggggaacta acgatttggt atccacatca agaggagatg
     2701 tgcgctttag agaggcaaaa attctcaatg caagcgccta tgacctttcc tatgcaagca
     2761 ctggaaagag acctggtgac ccgggctacg gaattacagc ctcaggaaca actgcacgac
     2821 ctggaaccgt tgcagtggat cctcgcgtca ttcccttagg gaccaagctt tacgtggagt
     2881 ccctggatgg atcgagagat tatggatttg ctatagcaga agatactgga ggagccatta
     2941 aaggcaacaa aattgacctg ttttaccatt caaacgccga ggcaatgaga ttcggccgaa
     3001 gaaatgtaaa ggtctatatt ctggactaag agaaaagata tcgcttttgc gatatctttt
     3061 tttagtcctt tatcttcagt tcaaagacct ttgtgctcac tgggtggttt tctattccat
     3121 gggggtggag gaattccccc tcatcacttg taaagccctc ctgattgaag atgcatcgta
     3181 ttgaaaggtc tctgaccctc cgctcatcct gggcctgaat atgctttttt ataactgaat
     3241 ggggtatcag gcaggaggtc tggtttgcat tgtggatcac cagtaggtag cctccatttc
     3301 ccctgattgt atatgctatt actggacatg tggcaccctc gtcaataaac cttaactttt
     3361 tcctaatctc atccgcctgg tccatcctga actcctgcct ctcttttctc aatcttatca
     3421 ggtccttgat atatttgaaa aggtcaaggt tttcctcctt tagtgaccag tccataccgt
     3481 ttatggacat tggggcattg taggtgtttt ccctgccatc cttggtcctc atgaactcat
     3541 ttccggcgtg gataaagggg actccctggg aaagtatcag gattgcatgg ataaacttgt
     3601 tgtactccac caggtctttt cttggcttgt ttggaaagac cttaagtagc ttgtcctgga
     3661 ggataaggtt gtcgtgggag ttggcatagt ttatggtctc ccttgccatg gaggcaaatc
     3721 cgattcttga ctcgtcgaaa aatatggagc ctgcaattcc cgtttcagtc gccctttttt
     3781 ggtccagatt tccctgggca aaccccttct cgt
//
LOCUS       DASRQF010000286         3700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1352237, whole genome shotgun sequence.
ACCESSION   DASRQF010000286 DASRQF010000000
VERSION     DASRQF010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352237"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VFD79_07660"
     CDS             <1..405
                     /locus_tag="VFD79_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93965.1"
                     /translation="KGGSIERNFELYKNAFILYSKGMEPITVESPAWSLDILPTLSNL
                     MGLDFDSRLMMGRDIFSDKPPLVFFSNRSFITDKGMFNSTTGNFRSFGDFESEDEKEG
                     YIRSVSETIDAKFLYSAWMLQTDYYKKVLGGF"
     gene            complement(429..1412)
                     /locus_tag="VFD79_07665"
     CDS             complement(429..1412)
                     /locus_tag="VFD79_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HZH93966.1"
                     /translation="MLQLKIVVLDGYTLNPGDLSWEGVSQFGDLTVYNRTAYKPGGED
                     LVVERSKDADVVLLNKTLLNREMISRLPNLKYVGVLATGYNIVDVEAARERGILVTNV
                     PTYGTTAVAQMTFALLLELCHHVGDHNRAVQNGEWVENPDWSFWNYPLVELAGKTMGI
                     IGYGRIGGEVGKIAQAFGMNVLAMASRPKPELETENLKFAPLEDILEKSDVISLHVPL
                     FDSTKGMINRESLEKMKKNAMIINTSRGPLIVEEDLADALNRGRIAGAALDVVSSEPI
                     NPDNPLLKAENCIITPHIAWAPKESRKRLMDVAVENLKEYIAGNPINVVNE"
     gene            complement(1908..2171)
                     /locus_tag="VFD79_07670"
     CDS             complement(1908..2171)
                     /locus_tag="VFD79_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93967.1"
                     /translation="MSVFEILMLLCFGAAWPFSIYKSYTSKSVKGKSPIFLLVLMSGY
                     VFGILNKLFYHYDHVIFLYLLNLVMISTDFGLYLRNKKLGEEC"
     gene            2330..3457
                     /locus_tag="VFD79_07675"
     CDS             2330..3457
                     /locus_tag="VFD79_07675"
                     /inference="COORDINATES: protein motif:HMM:NF017072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH93968.1"
                     /translation="MKIKSIGFPRMNKEESEKRDFVPLLFRLLQLNQDVEILMEEDYG
                     IGMGFGINDYLLVNDKIKILPRRKVFHADMVVVLRTPDEDELRWMRPGSVLFSMLHYD
                     TRESRNKLLQDLSLIPFSMDGIEDDWNKRMVVNYSGTSYSGVEIAFKELKKSTNDFEI
                     PGNRPLYAGILGFGSVGLEAAKALKHYSDSFFLNNRSKTPGMIINLYPRSITEKPEIL
                     KEELKKMDILVDASRRRDPSQYILKNSWLENMPLHGIILDLSADPYNTDISPIQVKGI
                     EGIPTGTLDQLVFNPEDPVYETIPEGVDTTHRRTVVSCNAWPGVDPIGCMTLYSKQLI
                     YFLRELISKDPEKLTIHSDDPYERALVRSSLEYFLDTQQKS"
     gene            complement(3493..>3700)
                     /locus_tag="VFD79_07680"
     CDS             complement(3493..>3700)
                     /locus_tag="VFD79_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HZH93969.1"
                     /translation="ALLVTEAPFITKFLVGIVSVSEVLFFSASIPCIIATEIPLTIKD
                     YLIIWVERVVISIIIAAPILHILF"
BASE COUNT         1083 a          810 c          758 g         1049 t
ORIGIN      
        1 aagggtggaa gtattgaaag aaactttgaa ctttataaga atgcatttat tctttactcc
       61 aagggtatgg agccaattac agtggaatcc cctgcctgga gtctggatat ccttcccact
      121 ctctcaaacc ttatgggtct ggactttgat tctaggctga tgatggggag ggatatattc
      181 tcggacaagc caccccttgt atttttttca aacaggagct ttattacaga taagggcatg
      241 ttcaattcaa ccactggaaa ttttagatca tttggtgatt ttgaaagtga agatgaaaaa
      301 gaaggctaca ttaggtctgt atcagaaaca atagatgcta aatttctcta ttcagcctgg
      361 atgctccaaa ccgactacta taaaaaggtg ctgggtggat tttagtccac cagcaccttt
      421 atttgttttt attcgttaac aacatttatt ggattaccag caatatattc cttaagattt
      481 tccacggcca catccatcaa cctctttctg gattcctttg gagcccaggc aatgtgtgga
      541 gttatgatgc aattctcagc ctttagaagt ggattatcag gatttatggg ctcagatgat
      601 acaacatcaa gtgcagctcc tgcgatcctt cccctgttca gtgcatctgc caggtcctcc
      661 tccacaatca ggggacctct tgatgtgttt atgatcatgg cattcttttt cattttctcc
      721 agtgattccc tgtttatcat ccctttggtt gaatcaaaaa gtggaacatg aagacttatc
      781 acatctgatt tttctaggat atcttccaaa ggagcaaact tcaggttttc tgtctcaagc
      841 tcaggttttg gcctgcttgc catggcaagc acattcattc caaaggcctg ggcaatcttt
      901 ccaacttcac cgcctattct tccatagcct attattccca ttgtctttcc tgcaagctcc
      961 acaaggggat agttccagaa ggaccaatca gggttttcaa cccattctcc attttgcact
     1021 gccctgttat ggtctccaac atggtggcaa agctcaagaa gaagtgcgaa ggtcatctgg
     1081 gctacagcag tggttccata ggttggtaca tttgtcacaa ggatgcctcg ctctctggcg
     1141 gcttcaacat caacaatgtt gtagcctgtc gcaagaaccc caacatattt caggtttggc
     1201 agtctactta tcatctccct gttcagcagg gtcttgttga gtaggaccac atctgcatcc
     1261 ttggatcttt caaccacaag gtcctctcca ccgggtttgt aggctgttct gttgtatacg
     1321 gtaaggtccc cgaattggga gactccctcc caggacaggt ctcccgggtt tagtgtgtat
     1381 ccatcaagta caacgatttt caattgcaac accgcctttc gtataattcg aattgtatta
     1441 tatttgtgtc agacttcgat tcggaaagaa taatgccaca ctctttcata taattcacaa
     1501 ttcacagttc acaatttaaa tgccaaagga aaattgaaga taattcctta gataaaggat
     1561 gccgacgata tctagctttc aaacacaata tagatgaaac gtcaccactt cttaagattc
     1621 ttcgattact ctcagaatga cacttaattt gtcaccctga gcttgcgaag ggtcttggac
     1681 cctatagctt gacccgagat gtctcgacaa gctcgacatg acattctagc tgtcatcccg
     1741 acctaaacgg agggatctct cttttcctga ccaactaaca accaactact aacctcttac
     1801 ttcttagata ttaactatct tctctattgc ttgtgcccgg cgatagattt cctaaaaatc
     1861 tatgattttt ctggaattca tcgagagggt atgaattgtg aattttccta gcattcttcc
     1921 ccaagtttct tgttcctcaa gtacagtcca aagtcagttg aaatcatgac aagattaagt
     1981 aagtacaaga atataacatg gtcataatga tagaacagct tgttcagtat cccgaataca
     2041 tacccactca taaggaccaa aagaaaaatg gggctctttc ccttcactga tttggatgtg
     2101 taggacttgt agattgaaaa tggccaagcc gccccaaagc aaagcaacat tagaatttca
     2161 aaaacgctca tatatacctc ccaggctaat tgatatattc ccattaacct aatcttacca
     2221 gattccaact tcctttgaca gtgtaatgtg gaaagttttg ctggagaagg tggtgaaata
     2281 tactctgtta gggtattata gaactaatac cccaaaggac ggtgattcaa tgaaaataaa
     2341 atcgatcgga ttcccaagga tgaacaagga ggagagtgaa aagagggatt ttgtaccctt
     2401 gcttttcaga ttgctgcaat taaaccagga tgttgaaata ctaatggagg aggattacgg
     2461 aattggaatg ggctttggca taaacgatta tcttcttgtc aacgacaaga tcaagatcct
     2521 tccaaggaga aaggtcttcc atgctgatat ggttgttgtt ctaaggacac ctgatgagga
     2581 tgaactaaga tggatgaggc ctggctcggt actgttttcc atgctccact acgatacaag
     2641 ggaaagcagg aacaagctac tgcaggacct ttccctcata cccttctcaa tggatggaat
     2701 agaggatgac tggaacaaga ggatggttgt aaattacagc ggaacctcct attcaggagt
     2761 agagattgca ttcaaggaac tgaagaaaag tacaaatgac tttgagatac ctggaaacag
     2821 gccactttat gctggcatac tgggctttgg atcagtgggg ctggaggcag caaaggccct
     2881 taagcactac agtgacagtt tctttctgaa caacaggtca aagacccctg ggatgataat
     2941 aaatctttat ccaaggagca taactgaaaa gcctgagata ctcaaggagg agcttaaaaa
     3001 aatggatatc ctggtggatg ccagcagaag aagagacccc tcccagtaca tactaaagaa
     3061 ttcatggctg gagaatatgc ctcttcacgg tattatactt gacctttctg ctgaccccta
     3121 taacacggat atctcaccca tccaggtaaa gggcatcgag ggaatcccca ccggcaccct
     3181 ggaccagctg gtgtttaacc ctgaggatcc tgtctatgag accatccctg aaggagtcga
     3241 cactacccat agaagaacag ttgtaagctg caatgcctgg ccgggagttg acccaatcgg
     3301 ctgcatgacc ctttacagca agcagttgat ctatttttta agggaactga tatcaaagga
     3361 cccagaaaaa ctcaccatcc attcagatga cccctatgaa agagccctag taagatcttc
     3421 actagaatat tttcttgata ctcaacaaaa aagctgaccc attgggccag cttttcattc
     3481 tgtgattcca tattagaata gtatgtgaag tattggtgcg gctataataa tagaaattac
     3541 aaccctctca acccagatga taagataatc cttgatggtc aaaggaattt ctgtagctat
     3601 gatacaaggt attgaagctg agaagaaaag tacctcagat acactgacga ttccaaccag
     3661 aaacttagtt ataaatggtg cttcggtaac caagagtgca
//
LOCUS       DASRQF010000287        11042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1153514, whole genome shotgun sequence.
ACCESSION   DASRQF010000287 DASRQF010000000
VERSION     DASRQF010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 11042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11042
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153514"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="VFD79_07685"
     CDS             <1..785
                     /locus_tag="VFD79_07685"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZH93970.1"
                     /translation="MQLCEAGRLSLQDKVSDHIPGQYRGDEITIENLLMHSSGVIRDL
                     TDTGAIGPYESIGFDELVEKIGDRSLLFDPGSSMKYSNAGYQLLGAIIERASGKSYGD
                     YIRSNIFEPAGMKSSGVVDRRDEVEGLAVGYEFDGFGFVEKEFYSMSHAYGSGNLYSS
                     AYDMYLFDSALRAGKLLNDESSNVMIDAGVESLYKYGYGCYVGELYESFWYGHPGNLN
                     SGYFSMYLRFPDEDIGVIVLLNTTWDDNNGIMLDTCKIALGI"
     gene            complement(835..2220)
                     /locus_tag="VFD79_07690"
     CDS             complement(835..2220)
                     /locus_tag="VFD79_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HZH93971.1"
                     /translation="MNTFYTILVLLAATGIVFALSQMQKRHMSFTRRVLTALVLGILF
                     GGSLQVIFGSGSDVVSASYSWISIIGSGYVRLLNMIVIPLVFVAITTSIVNQDTNALK
                     KSAASILLVLILTTAISAGLGAGVAGAFNLSSSDLVAGQAEASRGETLENTLNEFQSK
                     SIPEQIAEIIPRNIFYAMSGQGSSATLSVVFFSGFLGIATIGLRRKKPESAEAFTKFL
                     NVLHDIVMRMVTMVLRLTPYGVFALLSRFVSTSNFSEISRLFEFVLASYVAIMLMFLI
                     HLAILSAFRLSPLKYLKKGSVPLSFAFSSRSSAKTLPINVETQTERFGVSKGIANLSA
                     SLGTSIGQNGCAGIYPGMLAVMIAVGTGVPMDASFFVRLILITAISSFGIAGVGGGAT
                     FAALTVLSSMGLPVGLVGLLIAIEPLIDMGRTMLNVSGSMVAGLVTARRLGEIDLDVY
                     NRDFIDFEQTA"
     gene            2857..5250
                     /locus_tag="VFD79_07695"
     CDS             2857..5250
                     /locus_tag="VFD79_07695"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HZH93972.1"
                     /translation="MAGVLTATFLLWNDNDFDETILDTDNIINHIQELSSGAYEGRLA
                     GSDGDGRALKYIEDHFRELDIEPAGVEGTYYQPFSVMIPKIDTEPTFKVVSDDGKIIR
                     ELIMYEDYNALPSMNGGSIDFSGELVYLGSDLLRTDPELIRNRIVIIEANRLLPKHQE
                     YVIQAGGEGILLSADTNIFGRQLRYASEKFLNVEGKTGQSILTGYISNEAYRDLRRLV
                     EGGDKGDEKQTFPVLGGVRIKVDMGFPIVDTANIMGKIEGKGTDGSVLLISANIDGLG
                     EGTDGKYFPGVVNYTSGIATILEVARVMASQESIPYQTVVFVGWNGQQQQLAGSSHYI
                     DYPVFPMESSSLIHLEGLGKKSLEGIKISAHMANGAMLKDRVLDYSQDADLRAVEDSS
                     ITGVINQFNDSKAPAVLLRDTADVQDTYDDTIENIERDTLENSALVLLNHIKRDIYRD
                     RGFDYLTIIEKVLVAIVTFSSLVSYFIVAAYNREPNRRVFGRTLESIYYSTPAVLLRK
                     GMTLVVSLFIAIFMLSLLANIDPGTDVRTTSKGISSNFSLYLTIKRSVIYIRTMLSPG
                     SYQLTGTGNIFQIIYNSSKQSIMLISASLLISTFLGILRGMYEGYRSRKSRLGSLGTL
                     VFFSIPDVLIVLFTLLGYIFVFRKMPIVGEMEYIKGFLLPLFTLSIIPTIYISRITFI
                     TVQGEMGKDYTRNAMAKGFSRRKTVFTELAPAVLFKIVDTMPAIMTMLLTNMIVVEWL
                     FNYQGILYYLLYFNSRQDVYRFVPLAVVLGLVYVTATMGFQFIARLINPLKTRRANK"
     gene            5247..7505
                     /locus_tag="VFD79_07700"
     CDS             5247..7505
                     /locus_tag="VFD79_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZH93973.1"
                     /translation="MKKPNLPLILGTIILVVILLVMSFPDMFTDNSPYTIQLMRFIHE
                     DGKLSAERAPFPPGEDYLLGSDDLGRDIFSYIIHGTRLTISLGVLIAVGQFMVAIPLA
                     LLGGFGNRFARSVIRQSNIIFSAIPALLISIILLKLRFIANLDKQSSIIAFVLILTAV
                     SWPKLGSLVSERVEGILQQPFIRGEVAIGKRKFRIALENVLPHLGPELVILFFMEIAR
                     NLSMIMQLGIFSIFVGNLGLINDSSSGALINTDISFEPEWASMLSTSRTLVSTAPWAV
                     IFPALAFFISVLGFNLFGEGLRNIIQRKDSRMPLVFRKLISFDLNYVWNMVDRKRKIR
                     FAAAIIIVIGSFTALSFANKGDYSINLESDPASYPHEAIIGSGGIEQVVESITERMVK
                     LGIEPLREDYLMTYDIGPSFMIEDQRLGIDTGDEILQLIPNADFKFTTSRSNEDHGFV
                     YDATRVDLFNIEDFSGFADKFILIDKAFYNERSIDHFIGEVTEKVDIKGILLVAEKDE
                     RMDGFITDDRDELSVILVSRDIAGILESNPDSRISIYSSVKPLEPKGRNVVGILRGED
                     RNIGDEAIVVGMNYNYTRDDGSSLLQFNLELMERLCEQGGNKRSIIFMFLDGTIDEKR
                     HGINYMVNDFPYSPNKVEVYLDLTGLVETGFDRVHFSSAQAPVTRQFAWSMGHHLQKI
                     LESKGLELTQLQTRSVDNEYYFTGSQADNAMFWERGIPTIHLLTGETGKRTRNIQEVG
                     SILLEVINRNNY"
     gene            7547..8509
                     /locus_tag="VFD79_07705"
     CDS             7547..8509
                     /locus_tag="VFD79_07705"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZH93974.1"
                     /translation="MEDKLLDVRDLKVSFYSDKGSVQVVRGFDISMKKGEIVGILGES
                     GSGKTVSSSSIIKLLDESLGRIDSGEAIFKGRDLLKLSEKDLKRIRGDEISYIFQNSS
                     AALSPYKRIGKQLGEVLKTHGRVVSKEHILRTLEEVGLEDGERIYHMYPFQLSGGQNQ
                     RIMIAQCILCDPELLIADEPTSSIDASLQKTILELLKDISMRNKMSVIIITHDFGVAR
                     YLCDRLMVMYGGLVLEEGTIEEMLNSPLHPYTQELIRCARSLAEDDETMYTIEGAPPT
                     PYQFRDECPFYSRCSLREGACREGIPQKIDLGGRRVRCIRYEKR"
     gene            8522..9292
                     /locus_tag="VFD79_07710"
     CDS             8522..9292
                     /locus_tag="VFD79_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZH93975.1"
                     /translation="MILKVEKLSKHYIIKSGAFSKRQVIRAVQEVSFQLVENQSLGLI
                     GESGSGKSTVANLVLRLIKPSGGRIELFGEDITVMKEEEMRRYRKDIQIIFQYTNDVL
                     DPQMTVDELLKEPLKIHGIVKDGELDQEVDRLLEQVGLSPSERWKLPGQLSGGQNQRI
                     IIARAIATRARLIVCDEPVSALDVSVQGQILNLLIRLKEELNLSYLFISHDIKVIRHM
                     CDRIAVMKNGEIVEMGDVEDVLDNPQHEYTRKLIEAML"
     gene            9323..10222
                     /locus_tag="VFD79_07715"
     CDS             9323..10222
                     /locus_tag="VFD79_07715"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZH93976.1"
                     /translation="MSELKYKEKNLMNKELYEAVSELEEICRKHDDVRLKLELDYKLA
                     KAGTLEDRSEGKLDEFTCWDGDRLIGYLGIDDFGGGPIEVNGMVHPDYRRRGIFRDLL
                     SRVKMEWEIRNSNEMLLLTDRKSVAGKSFIQNTGAVYDHTEYEMILSDSLFERAKKST
                     MGLVLREATNDDSREIARQNAIYFNEDLADQELLDIELEKKRGFSVYMALLQGNIVGK
                     AHLHLVNGEGGIFGLGILPEYRGTGLGRELLLRSVEKLKELGATYIFLQVDAENDTAL
                     GLYRSCGFEESHSMEYHKMNKER"
     gene            10232..>11042
                     /locus_tag="VFD79_07720"
     CDS             10232..>11042
                     /locus_tag="VFD79_07720"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZH93977.1"
                     /translation="MELEGRIVELNNENLKDFTDFCRKHRDQVDDFNLYEEDLDSFQY
                     SPENPTFLYMEGKQVVAAASLIIDEYNRRGNRARFRILYSEKNNRAVYDTIFHRILIH
                     AIDLEKVYIFVPSSNIELMGRMEDLGFKAERFVYLMVRDDAPVPEDKIPEGYEIGPVE
                     LEEDYAGWCKVRNEAFAFVTGNEIPICTEMIMTYSDKEDYLPGGMMLLRHEGKPVGTV
                     RCIKDEYEGKEVVNIGSLSVLPEYQHKGLGSMLLREAIRFAHEAGFERTILS"
BASE COUNT         3317 a         2079 c         2750 g         2896 t
ORIGIN      
        1 tcatgcagct atgtgaagct ggacgactct ctctccagga caaggtctct gaccatatcc
       61 ctggacaata caggggtgat gaaattacaa tcgagaatct tctgatgcac tcatcgggag
      121 tgatcagaga ccttacagat actggtgcaa taggtccata tgagagcata ggatttgatg
      181 aacttgttga gaaaataggt gacaggtcac tactttttga ccctgggagt tcaatgaaat
      241 acagcaatgc aggttatcag ctcctgggag ctataattga gagggcttca ggaaagagct
      301 acggggacta cataaggtca aatatttttg aacctgctgg gatgaagtct tccggtgtgg
      361 tggatcggag ggatgaagtg gaaggccttg cggttgggta tgagtttgat ggctttgggt
      421 ttgtagaaaa ggaattttac agcatgtccc atgcctatgg ttcaggtaat ctctactcca
      481 gcgcatatga catgtacctc ttcgacagtg ccctaagagc agggaagtta ttaaatgatg
      541 agtcatccaa tgtaatgatt gatgcaggag tggagtcact gtacaaatat ggctatggct
      601 gctatgttgg agagctttat gaaagctttt ggtacgggca tccgggtaat ctaaacagtg
      661 gatacttttc aatgtactta aggtttccgg atgaagacat cggggttata gtactgttga
      721 acaccacctg ggacgacaac aatggaataa tgctggacac ctgcaaaatt gcactgggta
      781 tatgaatctg cccacggggt cagtccccgt gggcagatca taagagccta aatttcaagc
      841 agtctgttca aaatcaatga aatccctatt gtagacatcc aaatcaatct ctccaagcct
      901 tcttgcagtg acaagaccgg caaccatgga tccggaaaca ttcagcatgg ttcttcccat
      961 atcaatgaga ggctcgattg ctataagcag acccacaagg ccaactggca gccccatgga
     1021 agacagaact gtcagtgctg caaaggtcgc ccctccacca actccagcaa ttccaaagga
     1081 gcttatggcc gtgatgagaa tcagtcgtac aaagaaggat gcatccattg gaaccccagt
     1141 tcccacagca atcataactg ccagcattcc agggtagatt cccgcacatc cgttttgtcc
     1201 tatgctggtt cccaaggatg cggaaaggtt ggctataccc tttgacacgc cgaatctttc
     1261 ggtctgagtc tcaacattta taggcaatgt ctttgcgcta gacctggatg agaatgcaaa
     1321 ggaaaggggg acgcttccct tcttaaggta ttttaacggg cttagcctga aggctgaaag
     1381 aatagcaagg tgtatcagga acattaacat tattgcaaca taggaggcca gtacaaattc
     1441 gaaaagcctc gatatctctg agaagtttga agtggataca aatctgctca acaatgcgaa
     1501 aacgccatat ggagtaagtc taaggaccat ggtgaccatt ctcattacaa tgtcatgaag
     1561 cacgttaaga aattttgtga aggcttcagc gctttccggc ttcttccttc tcaggccgat
     1621 tgtggcaatt cccagaaatc ctgaaaagaa aaccacagaa agtgttgctg atgaaccctg
     1681 tccgctcatt gcatagaaga tatttctcgg tatgatttca gcaatctgtt caggtatgga
     1741 tttggactga aattcattca aggtattttc tagggtctct cctcttgatg cttccgcctg
     1801 acctgccaca aggtcgctgg agcttaggtt gaatgcacct gctacacctg cacccagacc
     1861 agccgaaatg gcggttgtca gtatcaggac aagcaatatg gatgccgcac tcttcttgag
     1921 ggcatttgtg tcctggttga cgatggatgt tgtgattgca acaaatacca aaggaattac
     1981 tatcatgtta agaagtcgca catagcctga gccaatgatg ctgatccagc tgtaggaagc
     2041 tgaaaccaca tctgatccag agccaaagat gacctgtagt gaacccccga acaaaatacc
     2101 caaaaccagt gcagtcaaga ccctcctggt aaaggacatg tgtctctttt gcatctggga
     2161 aagcgcgaag actatccctg tagctgccag taaaactaaa atagtgtaaa atgtgttcat
     2221 aattctcctc cttaagtgta gtatacttgg actcttaagt gatacgtctg gagggtttgg
     2281 aatgacctgg tggagctgtg caagtcaaaa ataaatcaaa aaaagctttc gcctctatat
     2341 cagaggcgaa agctaacttc cacggttcca ctctgtttgt ccaaaaaaat ggataaactc
     2401 aaaattaggt acaatcaata ccctaatgct ataacgggca tacccgctga tatctactat
     2461 gacttcgatc cagtactcag agatgcactt gggtccccat caaactaaga atcctcacag
     2521 ccaagggatt ctctttctgc aagttctcat agggacttac tcttctcatc atcgtattcc
     2581 gactaattca ctagacatta aatacaatta ctaatatatc ttataaattg acagatgtca
     2641 atatgaattc tctataatac atggtgaatg tattacaaaa caattacagt tctgcaaata
     2701 ttcagaatac ggtttaatgt ggtttaaaag tgcgatataa ttttaaaatg ggatgatgta
     2761 ggaatttcaa ggcggaatta cttaaaataa agtcgacggt aggtgaatat cttgcgtatc
     2821 tacaaaattg gaaaatggat taaatacata ctctttatgg caggtgtttt gacagctaca
     2881 tttttgctct ggaatgacaa cgattttgat gaaaccattt tggacacgga taatataata
     2941 aatcacatcc aggagctgtc atcaggggct tacgaaggta gactggcagg ctccgatggt
     3001 gatggaagag ccctaaagta tattgaggac catttcaggg agctggatat cgaacccgca
     3061 ggggttgagg ggacttatta ccagccgttt tccgttatga ttccaaagat agatacagaa
     3121 ccaacattta aagtggtttc agatgatggc aaaattatca gagaactcat catgtatgag
     3181 gattacaatg cccttccatc catgaatgga gggagtatag acttttcagg tgaactggtt
     3241 tatctgggta gtgacctctt aaggaccgat cctgagctga tcaggaatag gattgtaata
     3301 atcgaagcca acagactcct tcccaagcac caggaatatg taatccaggc aggtggagaa
     3361 gggatactct taagcgccga tacaaacatc ttcgggaggc agttaaggta tgcttctgaa
     3421 aagtttttaa atgttgaagg aaaaacagga caatcaatct tgaccggcta catatcaaac
     3481 gaagcctatc gtgacttgag aagacttgtg gaaggtgggg acaagggaga tgaaaaacag
     3541 acttttcctg tgcttggagg ggtcaggata aaggtggaca tgggtttccc aattgttgat
     3601 actgccaata tcatgggaaa gattgaagga aaagggacgg atggaagcgt tcttttaata
     3661 tcagcaaaca ttgacggctt gggggaggga actgatggta aatactttcc tggtgtggta
     3721 aattacactt ccggcatagc cacaatactt gaagttgcaa gggtgatggc aagccaggag
     3781 agtattccct accaaacggt ggtgttcgtc gggtggaacg gtcaacagca gcagcttgcc
     3841 ggttcaagcc actacatcga ctatccggtc tttcccatgg agagttcctc cctgatccat
     3901 cttgagggtc tggggaaaaa aagcctggag gggatcaaaa tttcagcaca catggccaat
     3961 ggagccatgc taaaggatag ggttcttgat tactcacagg atgctgattt aagggctgtt
     4021 gaggacagtt caatcacagg tgtcataaac cagttcaacg acagcaaggc accagcagta
     4081 cttttgcgtg atactgcaga tgttcaggat acatatgatg atacgattga gaatatagaa
     4141 agggataccc ttgagaactc agcattggtt ctacttaacc acatcaagag ggatatctac
     4201 agggaccggg gttttgatta cctcactatt attgagaagg tgttagttgc aatagtgact
     4261 ttcagttccc tggtaagtta ttttattgtc gctgcctata accgggaacc aaacagaaga
     4321 gtctttggaa ggacattgga aagcatttat tattctactc cagcagtcct tctgagaaag
     4381 ggtatgactt tggtggtgtc tttgttcata gccatattca tgctttccct tcttgcaaat
     4441 attgatcctg gcactgatgt tagaactacc agcaagggta tatcgtcgaa tttttctttg
     4501 tatctgacaa taaagaggag tgtaatatac ataaggacaa tgttaagtcc gggaagctat
     4561 cagcttacgg gaacaggcaa tatattccag ataatctaca actcaagcaa gcagagcatt
     4621 atgcttattt ctgcctctct cctgatttct acttttttgg gaatactcag aggaatgtat
     4681 gaaggataca ggtccagaaa atcaagactg ggttccctag gaacactggt cttcttctcc
     4741 ataccagatg tattgattgt actttttaca cttttgggct atatatttgt gttcagaaaa
     4801 atgcccatag ttggtgaaat ggagtacatc aagggctttt tactaccgct gttcaccctt
     4861 tcgataatcc caacaatata catttcaagg attacattta tcactgtcca gggtgagatg
     4921 ggcaaggatt ataccagaaa cgcaatggca aagggctttt caaggagaaa gaccgttttt
     4981 acggagcttg ctccggcagt tctattcaag attgtggata caatgcctgc aattatgacc
     5041 atgctcctga caaacatgat tgtggttgag tggcttttca attatcaggg aattctttat
     5101 taccttctgt atttcaacag cagacaggat gtttataggt ttgttccact tgcagtggtt
     5161 cttggtctgg tctatgtcac tgccacaatg ggatttcagt tcattgcaag acttatcaat
     5221 ccgttgaaaa caaggagggc caataaatga aaaagcctaa tctacctttg atactgggaa
     5281 ccataatact tgtagttatc ctattggtca tgtcattccc agatatgttc accgataaca
     5341 gcccctatac aatacagctt atgaggttta tccacgagga tggtaagctt tccgctgaaa
     5401 gagcgccatt tcctccagga gaggattacc tgctgggaag cgatgatttg ggaagggaca
     5461 ttttcagcta cataatacat gggacaagat tgaccatatc tcttggagtt cttattgcag
     5521 tgggacagtt tatggtggca attccccttg cacttttagg gggatttgga aacaggtttg
     5581 caaggtctgt gatcagacag tcaaacataa tcttttctgc cattcccgct ctgctgatat
     5641 caattatcct tctaaagcta agatttattg caaatctgga caagcaaagc tccataatcg
     5701 cctttgttct gatacttaca gcggtgagct ggcctaagct ggggagcctt gtttcagaaa
     5761 gggtcgaagg tattctacag cagcccttca taagaggaga ggttgcaatc ggaaaaagga
     5821 aattcaggat tgccctggag aatgtgctac cccaccttgg acctgagctt gtaatactgt
     5881 tctttatgga gattgcaaga aatctttcca tgataatgca gctggggata ttctcgattt
     5941 ttgttggaaa ccttggattg ataaatgact ccagctccgg tgcccttatc aatacagata
     6001 tcagttttga accggaatgg gccagcatgc tcagtacatc gaggaccttg gtaagcactg
     6061 caccctgggc ggttatcttt cctgccctgg cattttttat cagcgtgttg ggcttcaatc
     6121 ttttcggtga aggtctaaga aacataattc agaggaagga ttccagaatg ccccttgttt
     6181 tcaggaagct gatttccttt gatttgaatt atgtctggaa catggtggac aggaagagga
     6241 aaatcaggtt tgcagcagct ataataatag tgatcggttc atttactgca cttagctttg
     6301 caaataaagg tgactacagc ataaatctgg aatctgatcc cgcaagttat ccacatgagg
     6361 cgataattgg gtcagggggt atcgagcagg tagtcgagag tataaccgaa aggatggtca
     6421 agcttggaat tgaacctttg agggaggact atttaatgac ctatgacata ggtccctcct
     6481 ttatgataga agaccagagg ctaggaattg acactggtga tgaaatcctc cagcttattc
     6541 caaatgcaga tttcaaattt accacttcaa ggagcaatga ggatcatgga ttcgtatacg
     6601 atgccacaag ggttgacctt ttcaatattg aggactttag tggctttgca gataagttca
     6661 ttctcataga caaggccttc tacaatgaaa ggtcaattga tcacttcatt ggggaggtaa
     6721 ctgagaaggt tgatataaag gggattctac ttgtagctga aaaggatgag agaatggacg
     6781 gattcattac ggatgatagg gatgaactct cggttatact tgtttcaagg gatattgccg
     6841 gaatccttga atcaaatcct gattccagaa tttcaatata tagttcagtg aagcctcttg
     6901 agccaaaggg aagaaatgtt gtggggatcc taaggggaga agatagaaat attggtgatg
     6961 aagccatagt tgtgggcatg aactacaact atacaaggga tgatggaagc agcctgctcc
     7021 agttcaatct ggagcttatg gagagacttt gtgaacaggg aggcaacaaa cgatccataa
     7081 tcttcatgtt cctggatggg acaattgatg agaagcggca cggaatcaat tacatggtta
     7141 atgactttcc ttacagtccc aacaaggttg aagtctatct ggaccttacc ggacttgtag
     7201 agacggggtt tgacagggta catttcagct cggcccaggc tcctgtcact aggcagtttg
     7261 catggagcat ggggcaccac ctccaaaaga tactcgagag caaagggctg gagttgaccc
     7321 agttgcagac aaggtctgtg gacaatgaat actatttcac cgggagtcag gcagacaatg
     7381 caatgttctg ggaaagggga atccccacaa tccacctttt gacaggtgaa acggggaaaa
     7441 ggacccgaaa tatccaagag gtgggaagca tactacttga ggtgatcaac agaaacaact
     7501 attgatggat ggagagggta aagtggaaat atcaggggct gaaaatatgg aagacaaatt
     7561 attggatgta agggatttaa aggtatcctt ctacagcgac aagggcagtg ttcaggttgt
     7621 aaggggcttt gacatcagca tgaaaaaggg agagatagtg gggattctgg gggaatctgg
     7681 aagtggaaag acggtttcct caagctcgat aataaaattg ttggatgaat ccctgggaag
     7741 aattgactca ggagaggcaa tattcaaggg gagagacctt cttaagttgt ccgaaaagga
     7801 ccttaagagg attaggggag atgagatctc atatatattc cagaactcat cagcggccct
     7861 aagcccctat aaaagaattg gaaaacagct tggggaggtc ctaaagaccc atggaagggt
     7921 ggtttcaaag gagcatatcc ttaggaccct ggaagaggtt ggcctggaag atggagagag
     7981 gatctatcat atgtacccct tccagttaag tggtggtcag aatcagagga ttatgatcgc
     8041 ccagtgcata ctttgtgatc ctgagcttct tattgcagat gagcctacaa gctcaataga
     8101 tgcctccctt cagaaaacaa ttttggaact cttaaaggac ataagcatga gaaacaagat
     8161 gtcagtcatt atcattaccc acgactttgg ggttgccagg tatctttgtg acaggctgat
     8221 ggttatgtat gggggactgg tattggagga gggtaccata gaggagatgc tgaattctcc
     8281 acttcacccc tacacacagg agcttattag gtgtgcccgc tcattggcag aggatgatga
     8341 aaccatgtat acaatagagg gtgcccctcc aactccatat cagttcaggg atgagtgtcc
     8401 tttttacagc aggtgcagct taagggaggg tgcctgcaga gagggaattc ctcaaaaaat
     8461 agatttgggt ggaagaagag tgcggtgcat aagatatgag aagaggtgac aggccttgag
     8521 ggtgattcta aaagttgaga agctttcaaa gcattacata ataaaaagtg gtgcattctc
     8581 caaaaggcag gtaatcaggg ctgttcaaga ggtttccttt cagcttgttg aaaaccaatc
     8641 cctgggactg ataggtgaaa gtgggtcagg gaagagtaca gttgcaaatc ttgttctgag
     8701 attgataaag cccagcggtg gtaggattga gctgtttgga gaagatataa ccgtaatgaa
     8761 ggaagaggag atgcggaggt acaggaagga tatccaaatc atattccaat acacaaacga
     8821 tgtcctagat ccacaaatga cagttgacga gctactgaaa gaaccactga agatacatgg
     8881 aatcgttaag gatggcgaac tggaccaaga ggttgataga cttcttgagc aggttggact
     8941 gtcaccctct gaaagatgga agctaccagg ccagcttagt ggaggacaga atcagagaat
     9001 aataatagcc agggcaattg ccacaagggc caggcttatc gtatgtgacg agcccgtatc
     9061 agcactggat gtgtcggtgc aggggcagat acttaacctg ttgataagac tcaaggagga
     9121 gttgaacctg tcatatctct tcatttccca tgatataaag gtcatcaggc atatgtgtga
     9181 ccggattgcc gtaatgaaaa atggagaaat agttgaaatg ggagatgttg aggatgtact
     9241 tgacaacccc caacacgaat acactagaaa attgatagaa gccatgcttt gatatacaat
     9301 ataaccaggg ggttaagcaa ttatgtcaga attaaaatac aaagagaaaa atttgatgaa
     9361 caaggaattg tatgaggccg tatcagaact ggaagagatt tgcaggaagc atgatgatgt
     9421 aaggctgaag cttgagcttg actacaagct tgcaaaggca gggacccttg aggataggtc
     9481 tgaaggaaag cttgatgagt tcacttgttg ggatggtgat agacttattg gctacctggg
     9541 tattgacgac tttggaggag ggccaataga ggtgaatggc atggtccatc cagactacag
     9601 gagaaggggg atattcaggg acctcctttc ccgcgtgaaa atggagtggg aaataagaaa
     9661 ttccaatgaa atgctactcc tgaccgatag aaaatcagta gcgggtaaaa gcttcatcca
     9721 aaacacagga gcggtttatg atcacacaga gtatgaaatg attctttctg acagcctgtt
     9781 tgaaagagcg aaaaagtcta caatgggcct ggtcctaagg gaagcgacca atgatgattc
     9841 aagggagata gccagacaga acgccatata tttcaacgag gaccttgccg atcaggagct
     9901 tttggacatc gaacttgaaa aaaagagagg atttagtgta tatatggccc tacttcaggg
     9961 taatattgta ggaaaggcac atctccatct tgtcaatggc gagggaggca tctttggact
    10021 tggtatactt ccggagtaca ggggaacagg acttggaagg gagttgttgc taaggtctgt
    10081 tgaaaagctc aaagagcttg gggcaaccta catattcctg caagtggatg ctgaaaatga
    10141 tacggcactt ggattgtacc ggtcctgtgg atttgaagaa agtcattcga tggagtacca
    10201 taaaatgaac aaggaaaggt agggtggatt gatggaatta gaaggcagaa tagtagagct
    10261 taacaatgaa aatttaaagg atttcacaga tttttgcaga aagcacaggg accaggtgga
    10321 tgatttcaat ctttatgagg aggacctgga cagcttccag tacagccctg aaaatcctac
    10381 attcctttac atggagggaa aacaggttgt ggcagctgca tccctgataa tcgatgaata
    10441 caataggaga ggtaacaggg caaggttcag gatactttat tccgagaaga acaatcgggc
    10501 tgtctatgac accatcttcc acagaatact tattcatgca attgatctgg agaaggtata
    10561 catttttgtc ccatcatcta atattgagct gatgggccgt atggaggacc tgggcttcaa
    10621 ggctgaaaga tttgtatatt taatggtaag ggatgatgct cctgtacctg aggataagat
    10681 tcctgaagga tatgaaatag gacctgtaga attggaagag gattatgcag gctggtgtaa
    10741 ggtaagaaat gaggcgtttg catttgtaac aggaaatgag attccaatat gcactgaaat
    10801 gatcatgacc tattccgaca aggaggacta tcttcctggg ggcatgatgc tcttgaggca
    10861 tgaaggaaag ccagttggta cagtaaggtg catcaaggat gaatatgaag gaaaagaggt
    10921 tgtgaacata ggttcactgt ctgttttgcc agaataccaa cacaagggac taggaagcat
    10981 gctgcttagg gaagccataa ggtttgccca tgaagctggc tttgaaagga caatactgtc
    11041 cg
//
LOCUS       DASRQF010000288         3145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1807612, whole genome shotgun sequence.
ACCESSION   DASRQF010000288 DASRQF010000000
VERSION     DASRQF010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1807612"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="VFD79_07725"
     CDS             complement(<1..1056)
                     /locus_tag="VFD79_07725"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZH93978.1"
                     /translation="MALELSKKGMSIEPSVTLAISAKASAMKKAGENVISFSVGEPDF
                     NTPENIQKAGIEAITTGITKYTPASGTPEIKEAICKKFKNENGLEYKPSQIIVSNGGK
                     HSLYNALMAILNPGDEVIFSIPYWVSYPELVMIAGGVPVKLPTKEENEFKFSSADLEA
                     AKTDKTKAIIINSPSNPTGSVYSKEELQDIADWAVKNNIIVISDELYEKLIYDGYEHI
                     SIASLNEDIKNLTIVVNGMAKAYAMTGWRIGYAAGDEKIIKIMSNLQSHTTSNPNSIA
                     QYASIEGLSGSQDGIMKMKAEFDKRRKVMVDLINGIKGVSCATPKGAFYVMLNISQLL
                     GAEVEGVKINSSVDFADL"
     gene            complement(1068..1451)
                     /locus_tag="VFD79_07730"
     CDS             complement(1068..1451)
                     /locus_tag="VFD79_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZH93979.1"
                     /translation="MYKGERQRQFPTTISTTSFVKLYVLHLLNQKNYYGNRLKDEISR
                     RLGGKWTPSPGMIYPMLNTLEQEGYIVGHWEEPTKRTKRIYSITEEGRKHYETIRRMN
                     KSAFDDSLYIINNVLKDIYNITTNN"
     gene            complement(1451..2257)
                     /locus_tag="VFD79_07735"
     CDS             complement(1451..2257)
                     /locus_tag="VFD79_07735"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZH93980.1"
                     /translation="MRIDMHTHTTASDGLLTPTELVKKAFEKRLDGIAITDHDSVDAL
                     EEAIEKSMDYKGFLVIPGVEMGCNYDEEEVHILGYFIDYKDVRLRDTLSSLKNSRWDR
                     GILILDKLIQLGLSIPKDKILEKSRETGFIGRATIARELVNNGHVRDINEAFDKYLDV
                     GKPAYVDRYKLSVGDTINLIHDCGGVSVLAHPGILKNKDIMTYCVKRGIMGIECYHSK
                     HTKSDEKMVKSFAERNGLIQTGGSDFHGEKDILGDYSTDISTIPTLRERL"
     gene            complement(2341..>3145)
                     /gene="rny"
                     /locus_tag="VFD79_07740"
     CDS             complement(2341..>3145)
                     /gene="rny"
                     /locus_tag="VFD79_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HZH93981.1"
                     /translation="VLSGFDPIRREVARIALEKLIVDGRIHPARIEEMVEKAQKEVEN
                     TIREAGEQASFDAGVHGLHPEIIRLLGRLKYRTSYGQNVLKHAVEVSHLAGVMATELG
                     ADVKIARRAGLLHDLGKSIDHEVEGPHVEIGVDIARKYKESKEVLHAMEAHHGDVEPQ
                     TVEAVLVQAADAISAARPGARRETLESYIKRLEKLEEIANSFTGVERSFAIQAGREIR
                     IMVRPEEISEAQMIHTARDIVKKIESELDYPGQIKVNIIRETRTVEYAK"
BASE COUNT          786 a          727 c          532 g         1100 t
ORIGIN      
        1 aagatcagca aaatcgacgg atgaatttat tttgacaccc tccacttctg ccccaagtaa
       61 ttgtgaaata tttaacatta catagaatgc accctttgga gtagcacagc tgactccctt
      121 tataccatta ataagatcta ccatgacctt cctacgtttg tcaaactcag ccttcatttt
      181 cataatgcca tcctggcttc cacttaggcc ttcaatgctt gcgtattggg caattgaatt
      241 tgggtttgag gttgtatgac tttgcaggtt actcatgatt tttattattt tctcatcacc
      301 tgcagcataa ccgattctcc atcctgtcat tgcatatgcc ttagccattc cgttaacaac
      361 tatggtaagg ttcttgatat cttcattaag ggaagctata cttatatgct catatccatc
      421 atatataagc ttttcataaa gttcatcgga tataactatt atattgtttt ttacagccca
      481 atctgctatg tcttgaagct cctctttgga atagaccgag ccagttggat tacttggact
      541 gtttataatt attgctttgg tcttatctgt tttagcagct tccaaatcag ctgaactgaa
      601 cttgaattca ttttcttcct ttgttggtag cttcacagga acaccacctg ctatcattac
      661 aagttctggg tagcttaccc aatatggaat actaaagatc acttcatctc caggatttaa
      721 aatagccata agtgcattgt aaagagaatg cttacctccg ttggagacaa ttatctgtga
      781 aggcttgtat tctagtccat tctcattctt gaacttctta cagattgcct ctttgatttc
      841 aggagtacct gaggcaggag tatattttgt tattcctgtt gttattgctt caatacctgc
      901 tttttgaatg ttttctggag tattgaaatc aggttcccct acactgaaac tgataacatt
      961 ctctccagcc tttttcattg cgctagcctt ggctgaaata gccagcgtaa ctgatggttc
     1021 aatagacatc cctttttttg ataattccaa agccataatt aaccctccta gttgttagtt
     1081 gtaatattgt aaatatcctt caaaacattg ttgataatat ataatgaatc gtcaaatgct
     1141 gacttgttca ttctccttat ggtttcgtag tgcttacgcc cttcttcagt tatgctatag
     1201 attcttttgg ttctctttgt gggttcttcc caatggccaa ctatgtagcc ttcctgttcc
     1261 agggtgttta acatggggta tatcattcct ggactgggag tccacttccc acctagcctt
     1321 ctgctaattt catccttgag cctgttgcca taatagttct tctgattgag caagtgaagg
     1381 acatatagct taacaaagga tgtggtactt atagtcgttg ggaattgtct ttgcctttca
     1441 cccttgtaca ttataatctc tccctgagcg taggtatagt actaatatcg gtggaatagt
     1501 cccccaatat atccttctct ccatggaaat cagatcctcc ggtttggatt aatccgtttc
     1561 tttctgcgaa ggatttaacc attttttcat cacttttggt gtgcttggaa tggtaacatt
     1621 ctattcccat tatacctctt ttaacacagt atgtcatgat atctttattt ttaaggatgc
     1681 caggatgggc tagtaccgat actcccccac aatcatggat tagattgata gtatcaccga
     1741 ctgatagctt atacctgtct acgtaggctg gcttgcctac atccaggtat ttatcgaagg
     1801 cttcattgat atccctgaca tggccgttat tgacaagctc ccttgcaatc gtagcccttc
     1861 caataaatcc agtttctctt gatttttcca gtattttatc cttgggaatg gacagaccta
     1921 actgtataag cttgtcaagg atcagaatac ccctatccca tctggaattt tttagtgatg
     1981 aaagagtatc cctaagcctt acatccttat agtcaataaa atagccaagt atgtggactt
     2041 cctcttcatc gtaattacaa cccatttcca caccaggtat aactaaaaaa cccttataat
     2101 ccattgattt ttcaatagcc tcctccagcg cgtcaactga gtcgtgatcg gttatggcaa
     2161 ttccatcaag ccttttctca aaagccttct tcaccaattc tgtcggcgtc agtaatccat
     2221 cagaagctgt tgtatgggta tgcatgtcaa ttctcattgc tgacctcctt gcacctatag
     2281 ttaaagcctg ccgaaatcgg caggctttat ttagcatcaa tactagttgt taaggaagtt
     2341 ctactttgcg tattccacag tcctagtttc cctaataata ttgaccttaa tctgtcctgg
     2401 gtaatccaat tcagattcaa tctttttaac aatatcacgt gctgtgtgaa tcatttgtgc
     2461 ttcacttatt tcttcaggtc ttaccattat tctaatctct ctacctgcct gaatagcaaa
     2521 tgatctttct actcctgtaa atgaatttgc aatctcctcg agtttctcaa gtctctttat
     2581 ataagattcc agagtttctc ttctggctcc aggtctagca gccgatattg catcagcagc
     2641 ctgtaccagt accgcttcaa cagtttgtgg ctctacatct ccatgatgag cttccatggc
     2701 gtgcagaact tctttgcttt ccttatattt tcttgcaata tccacaccta tttcaacatg
     2761 tggtccttca acttcgtggt caatggactt tcctaggtcg tgaagtaaac ctgctcttct
     2821 tgcaatttta acatctgcac ctagctcagt agccataacc cctgccaagt gagaaacctc
     2881 gactgcatgc ttaaggacat tttgaccata actggttctg tatttaagtc ttcccagcag
     2941 ccttatgatt tctggatgca atccgtgaac tccagcatca aaggatgcct gttctcctgc
     3001 ttctcgaatg gtgttctcaa cttccttctg agctttttca accatctctt cgattcttgc
     3061 aggatggatt ctaccgtcta caataagctt ctccagtgct attctggcaa cttcccttcg
     3121 aattgggtca aagcccgata gaact
//
LOCUS       DASRQF010000289         9185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1141167, whole genome shotgun sequence.
ACCESSION   DASRQF010000289 DASRQF010000000
VERSION     DASRQF010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 9185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9185
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141167"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="VFD79_07745"
     CDS             <1..1089
                     /locus_tag="VFD79_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZH93982.1"
                     /translation="GTPEAQDAFKNILKSEGFDISKLNVQVTDGGSTGMELVILGVCG
                     PAGVDEKPLMMIDPAYTNYISFAQRLGRKTVTIKRQMSEDGKFNLPEISEIEAMIKEH
                     NPGGLLVIPYDNPTGQLYDNKTLVELAKLCVDNNMWLISDEAYRELYYKEDQDLVSIW
                     ALSDEDVPGIEGRRISIETASKVWNACGLRIGAVITDNAEFSKKSIAEYTANLCANVI
                     GQYIFGALAHESKEDILGWCKEQRDYYKDMIVKVRKGLLEKEPDLVVSSPDASIYSVI
                     DVRNVVKPGFDSIDFVLYCAGEGSVEIDGVETTLLVAPMKGFYDIPEGEHNPGATQFR
                     ISFVESPENMAKVPHLFVELLRKYEAQR"
     gene            complement(1117..2364)
                     /locus_tag="VFD79_07750"
     CDS             complement(1117..2364)
                     /locus_tag="VFD79_07750"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZH93983.1"
                     /translation="MSELATKYVKKGELRNLVLFSAGKSVSMFASSIYSFAIGLYVLN
                     LTGSALNYATTIMLHILPMILVSPIAGVVADKIPKRNLIVGMDVANGFLFLALLIISS
                     KGGLSLSAIYLTTILLSVFSSIFNISFEASKPNLVRPESRLKLNSMSKLIDSTTSILG
                     PTLGGVMFALIDIQLFILINCISFFLSAISEMFIDYNFHPDTETLDNPPFMSQLAKGT
                     KFMLHSRVIRKVFILFVIINFLLGFSVNVPMPYIINQVLGLPPEFFGFISSMFPLGLI
                     FGTLTIGRVLKKGSYFNILTITTCLLALLASIVGLPLAFGTYGIMVYGIYYAGLNILM
                     GIAISYVDIPILTIMQDEIPPGLRGVVFGLTMSLVKVVLPISLLVSGYLVDYVPIALI
                     SIVGGALALVYPLHLLIKKTGSA"
     gene            complement(2491..3717)
                     /locus_tag="VFD79_07755"
     CDS             complement(2491..3717)
                     /locus_tag="VFD79_07755"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZH93984.1"
                     /translation="MRIGEFAVKNNTTIDTIRHYMDLSLLIPDKAGPYYEFDSNCQKD
                     YDTILSLKHIGFTLAEIQTLMLYERIGRHTEYSRKKTYRAFFYNKLQWIEEELYRLNS
                     MKDRLQGTLKSMPLESGHSHPQSGVPFKALPLLYCNECGTSYVMSEGQVENGQVISGK
                     LTCSCENSLKIRDGILYGKGALHHDMCDDIGDTFIDDYVKTTHVDYLKNLFMSLQWAR
                     NNYAFSDHKDGVILELGSGRGFFLRNVIDLLSKDSLYIAVDHNPRSQAWLKESLADVI
                     GNGNILFLCCDFKTIPLRPESLAAMIDATGSSNYAFEHEDFLLDSLVDLLADGSTIYG
                     HYLVFKNFAFNSIIPEKSRKWFKKESVKDQLAVLGFNHLKEYETSPISQGGPRESFFV
                     EGEKIFNYLYIGKLCR"
     gene            3943..4644
                     /locus_tag="VFD79_07760"
     CDS             3943..4644
                     /locus_tag="VFD79_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZH93985.1"
                     /translation="MSELNKTYEMVFKRMIKETDEIYSFVFEKPEELKWIPGQHAIFR
                     NMECKVEGDKNYRIFSFASIMEEDIVMFSTRITDESTECKKKLLGLKPGDRMTAEDPQ
                     GRFRIEDLDKPTCLMAGGIGITPIRALLKQMDFNCTNPRNLRVLYSDDRGEFAYEDTL
                     KWINRKYDGLDLKLISDRHEFMEEIESFAKEMRNNADYLISGTPGMNKAITDKLIALG
                     VEKDKIKTDNFIGYR"
     gene            4682..5974
                     /locus_tag="VFD79_07765"
     CDS             4682..5974
                     /locus_tag="VFD79_07765"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HZH93986.1"
                     /translation="MLQRILLKFRKSVWIYPSLISFISLVGAVLVGLADSGSFVNIPE
                     LVPEFLLTSVELSKDILGVIAGSLITMTTFTFSTTMVVLTTYSSQFSPRTVENFLSDD
                     DTMKALGVFMGGFVYSIVTLFFMKNSLGDQLVVSATLGIAYAIICLVQFTMYIHHVGS
                     YIQTNNLLDRLFQEAEKKIDDYIDLLEGGRLIHKSNWGGEDFVIRIRSRLDGYIQLVD
                     HRAIYRTAKDIKGTVVFEKINGQFVTDGSVLFSIYFQEEVQVEEESIQRLLDSITIGK
                     QKSEIQDFNFSMQKIAEIALRAISPGINDPNTASHCLRIMGVLLGKIAHIEGEFMVYQ
                     EEEDYPKVAYEIIEFSQEMRSSYHQILHYGKEDISVMQSMLKSIRFAAEKATGGNRKS
                     IGKYLDHVWEIIEKTHDNDFDLSVLENEKKDILTIIEG"
     gene            6064..6537
                     /locus_tag="VFD79_07770"
     CDS             6064..6537
                     /locus_tag="VFD79_07770"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HZH93987.1"
                     /translation="MNFDGKQILSTFVKMEEEVSKYYAEMAKNAPDEKSKALFERLSL
                     EEDKHNKMYAKLLEKHGGEMAREFSEEEIEYTKSLIDENINAKHEFDKNKKFGDSLEL
                     AERMEKDGILFVHQMMEMYPDIANKEMKVILMEEKKHLQQVRERMNFGPLRSLGL"
     gene            6677..7993
                     /locus_tag="VFD79_07775"
     CDS             6677..7993
                     /locus_tag="VFD79_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HZH93988.1"
                     /translation="MIQLGSDGSLTGKLILILVLTLVNAFFAASEMAMVSVDRIKLSS
                     KADKGDKKAKMLVNILKEPSRFLSTIQVGITFAGFFSSASAAVSISARLGDALDRMGI
                     PFGQDLAFIGITLILSYVMLVFGELVPKRIALRYAEKFAMLAVKPINLVAKIMHPFVT
                     FLSISTNTVMRIIGISQEGVEAKVTLEEIGSMVEVGHEQGFINPVEREMIRSVIKFDD
                     KLAEEIMTARTEVFAIDIDDPPEEYMEEFLRLKHSRVPVYREDVDNIIGILYLKDYLL
                     EAYRKGFGNIDIMDILRPAYFVPERKNINDLFMELKSTRKHMAILIDEYGGFSGLVTM
                     EDLIEEIVGDIDDEFDLDDPDVKKLDDNLYLARGSVSIKELNSRTGLELDEESEDYDT
                     LAGFLIHQLGYIPDDQETPEIMVDQVLFKIQEIEDKRITKVLIHST"
     gene            8114..>9185
                     /locus_tag="VFD79_07780"
     CDS             8114..>9185
                     /locus_tag="VFD79_07780"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZH93989.1"
                     /translation="MLRISGIRLGLDQEEGSIPSEISKKLRISENEVKAWRIWKKSVD
                     ARRKSNISFVYTVDFKTANDKAILKKFGKKGVSLASVENREYEKKTSIKGSRPVIVGT
                     GPAGLFAGLVLSEMGYNPILLERGKRIEERVKDVREFWETGRLNTESNVQFGEGGAGT
                     FSDGKLTTMTNDPRSRKVLEYFVEAGAPEDILYVNKPHIGTDILRRVVINIRKRIISL
                     GGEVRFESKVTGFEIEDGKVKAVVINQGERLQTERVVLALGHSARDTFQMLYENEFHI
                     QQKSFSMGVRIEHPQVLVDISQYGESHGHPRLGAADYKLAGHFENGRSAYTFCMCPGG
                     TVVAAASERWMLVTNGMSEHARN"
BASE COUNT         2882 a         1666 c         2251 g         2386 t
ORIGIN      
        1 ggaactcctg aggcacagga tgccttcaag aacatactta agagcgaagg gtttgacata
       61 agcaagctga acgtccaggt aaccgacggt ggatctacgg gaatggagct tgtaatcctt
      121 ggtgtttgtg gtcctgcagg agtggatgaa aaaccactta tgatgattga cccagcctat
      181 acaaactaca tttcatttgc acaaagactt ggaagaaaga cagttacaat aaagagacag
      241 atgagtgagg atggaaagtt taacctgcct gaaatttctg agatagaagc aatgatcaag
      301 gagcataatc ctggtggatt gctggttatc ccatatgaca acccaactgg ccagctttat
      361 gacaacaaga cattggttga gttggcaaag ctttgtgttg acaataacat gtggctaata
      421 agcgacgaag cctacagaga gctttactac aaggaagacc aggacctagt tagcatctgg
      481 gccttgtctg atgaggatgt accaggaatt gaaggaagaa gaatcagcat tgagactgca
      541 tcaaaggtct ggaacgcatg tggacttagg ataggtgccg ttataacaga caatgctgaa
      601 ttcagcaaga aatcaattgc cgaatacaca gcaaaccttt gtgcaaatgt aattggtcag
      661 tatatctttg gagcactggc acacgagagc aaggaagata tccttgggtg gtgcaaagag
      721 caaagggatt actacaagga catgattgta aaggttagga agggccttct tgagaaggag
      781 cctgatcttg tggtttcaag tcctgatgca tcaatctatt cagtaattga tgttagaaat
      841 gttgtcaagc ctggctttga ttcaattgat tttgttcttt actgtgcagg tgaaggatca
      901 gttgagattg acggtgttga gacaacactc ctggtggctc ctatgaaggg cttctatgat
      961 ataccggaag gagagcacaa cccaggtgct acacagttca gaatctcatt tgtggagtct
     1021 ccagaaaaca tggcaaaggt accacatttg tttgtagagc tcttgagaaa atatgaggct
     1081 caaagataag aataatgaaa gggtctgtct gattaatcag gcagaccctg tttttttgat
     1141 caaaaggtgc aggggataga ccaatgccaa ggctcctcca actatggaaa tcagggcaat
     1201 cggtacatag tccacaagat accccgagac cagaagtgaa attggaagaa caaccttgac
     1261 caggctcatg gtgagaccga acacaacacc tcttaggcct ggggggattt catcctgcat
     1321 aatggtcaat attgggatat caacatatga aatggctatt cccatcagga tattcaatcc
     1381 tgcataataa attccgtaga ccatgatacc ataggtacca aaagccaggg gtaggccgac
     1441 aatggaggca agaagggcca agagacaggt ggtgatggtc aggatattga agtatgaccc
     1501 cttttttaat acccttccaa tggtcaaggt accgaatatt aggcccagtg gaaacatgga
     1561 gctgataaag ccgaaaaact caggcggaag acccaatacc tggtttatta tataaggcat
     1621 ggggacgtta accgagaatc caagaaggaa gtttattatt acaaagagta taaacacctt
     1681 ccttatgacc ctggaatgga gcatgaactt ggtccctttg gccagttggg acataaaggg
     1741 tggattgtcc aatgtctcag tgtcaggatg aaaattgtaa tcgataaaca tttctgaaat
     1801 ggctgatagg aaaaatgaaa tacagtttat caggataaac agctggatgt ctataagggc
     1861 aaacatgaca ccacccaagg ttggtcctag tattgaggtt gtggaatcga tcaacttgct
     1921 catcgaatta agcttaaggc gactttccgg ccttacgaga ttcggcttgg aggcctcaaa
     1981 tgaaatattg aatatacttg aaaatacgct taagagtatg gttgtcaggt atatggcaga
     2041 caggctcaag ccacccttgc tgctgataat aagaagggca agaaacagga acccattggc
     2101 aacatccatc cccacaataa gatttctttt tggtatcttg tcggctacaa ctccagctat
     2161 tggactgaca aggatcattg gaagaatgtg aagcatgatg gttgttgcat agttaagtgc
     2221 cgacccggtg agattaagga cgtaaagtcc aattgcaaag ctatagattg atgaagcgaa
     2281 cattgagact gattttcctg ctgaaaagag tacaaggttt ctaagctcac ctttttttac
     2341 atattttgtt gccaactcac tcataattat tcccctttcc aagtctatgg tcagagtata
     2401 ctatgtggtg tgagcccaac tgcaacatgg tttatcaaat aatttggcac aaaaaacaat
     2461 ggagcatggg gttgacccca tgctctttaa ctacctacac agttttccaa tatacaggta
     2521 attgaatatc ttttccccct caacgaaaaa gctttcccta ggcccccctt gggaaattgg
     2581 agaggtctca tattccttta gatggttgaa gccaagtacc gccagctggt ctttcacact
     2641 ctccttcttg aaccatttcc tgcttttttc aggaattatt gaattgaagg caaaattctt
     2701 aaaaacaaga taatggccat aaatggttga accatctgca agtaggtcca ccagggagtc
     2761 cagaagaaaa tcttcatgtt caaaagcgta gtttgagctt cctgtggcat ctatcattgc
     2821 tgcaagtgat tccggtctaa gggggatggt cttaaagtca cagcatagga agaggatgtt
     2881 cccattgcct attacatcag ccagggactc ctttaaccag gcttgagatc tgggattgtg
     2941 gtcaacagct atatacagtg agtcttttga taagagatca atgacattac gtaagaaaaa
     3001 tcccctgcca gaccccaatt caaggataac cccatccttg tggtcggaaa aagcgtagtt
     3061 gttacgcgcc cactgcaggc tcatgaatag gttcttcagg taatccacat gggttgtttt
     3121 tacgtaatca tctataaatg tatcacctat gtcatcacac atgtcatgat gaagggcacc
     3181 cttgccatag aggatcccat cacgtatttt aagggaattc tcacaggaac aagtcagttt
     3241 ccctgatata acctgtccgt tctccacctg accctctgac attacgtagg aagtcccgca
     3301 ctcattgcaa tatagaaggg gaagtgcctt gaagggaact cccgattggg ggtggctatg
     3361 tccggattcc agtggcatgg atttcagggt accctgaagc ctatccttca tggaatttag
     3421 tctatacagc tcttcttcaa tccactgcag cttgttgtag aagaaggccc ggtaggtttt
     3481 tttcctgctg tactccgtat gtctgccgat tctttcatag agcataaggg tctggatttc
     3541 agccagggtg aacccgatgt gctttaggga taggattgtg tcgtagtctt tttgacagtt
     3601 agagtcaaat tcatagtatg gtccagcctt gtctggtata agcaaggata ggtccatata
     3661 gtgcctgatt gtatctattg tggtattatt cttgactgca aattctccta ttctcatggc
     3721 atcactccca atctggtctg atactatgat tataacaata atttctcaag tataaacaaa
     3781 aataccataa atggactcaa aaatacaaga aaaccgcgaa atctggaaat aatctgattt
     3841 tttatgataa ataatcaata atcttaaaaa taagggtata aactggttga agaagcatta
     3901 ggataggtag ggagacaaaa gggtgtaatg gaggtgtttt ggatgtctga attaaacaaa
     3961 acttacgaaa tggtttttaa gaggatgata aaggaaactg atgagattta ttcctttgtc
     4021 tttgagaaac cggaggaact gaagtggata cctggacagc atgctatttt caggaacatg
     4081 gagtgtaagg ttgaagggga caaaaactac aggatattca gctttgcatc cataatggaa
     4141 gaggacattg taatgttttc aacaagaatt acagacgaat caaccgaatg caaaaagaag
     4201 ctgctgggtt tgaagcctgg tgacaggatg actgccgagg acccacaagg caggttcagg
     4261 attgaggatt tggacaagcc cacatgcctg atggcaggag gcataggaat aactcccatc
     4321 agggcactgc tcaaacagat ggatttcaat tgcactaatc caagaaacct tagggtgctc
     4381 tattcagacg atagggggga attcgcctat gaggataccc ttaagtggat caacagaaaa
     4441 tacgatggat tggaccttaa actgatctcc gacagacatg agtttatgga ggagattgaa
     4501 agctttgcca aggaaatgag aaacaatgca gattatctta tttcaggcac accgggaatg
     4561 aacaaggcaa ttacagataa actgatcgcc cttggagttg aaaaggacaa gatcaagaca
     4621 gacaacttca taggctacag ataatcctta agacaaacac gacacaattt tggaggggga
     4681 catgctgcag agaatattgc ttaaattcag gaaaagcgtc tggatatatc catccttaat
     4741 cagtttcata tcattggtgg gagctgtctt ggttgggttg gctgattcgg gcagcttcgt
     4801 gaatattcct gagttagtgc cggagttttt acttacaagt gttgaacttt caaaggatat
     4861 actgggtgtg atagcagggt ccttgatcac aatgaccaca tttacttttt caaccaccat
     4921 ggttgttctg accacctact catcacaatt ttcacccagg acagtggaga atttcctgtc
     4981 agacgatgac acaatgaagg ccctgggagt attcatggga ggctttgtat actcaatcgt
     5041 aaccctcttc tttatgaaga acagccttgg agaccagctt gtggtatctg cgaccctagg
     5101 gattgcatat gcaatcatat gcctggtcca gtttacaatg tatatccatc acgttgggtc
     5161 ctatatacag acaaataatc ttttggacag actattccag gaagctgaaa agaagatcga
     5221 tgattatata gacctgttgg agggaggcag gttgatccac aaatcaaatt ggggaggaga
     5281 ggattttgta atcaggatca gaagcaggct agatggatat attcagcttg tggatcacag
     5341 ggctatctac aggacagcca aggacatcaa gggaacagtg gtttttgaaa agataaatgg
     5401 tcagtttgtc actgatggtt cagtcctctt ctccatctat tttcaggagg aggtccaggt
     5461 tgaagaggaa tccatccaaa ggcttttgga ttcaattaca ataggaaagc agaaatcaga
     5521 aattcaggac ttcaatttct ccatgcagaa gatcgcagaa atagccttaa gggcaatatc
     5581 tcctggtata aacgatccca atactgccag tcactgtctt aggataatgg gagtgctcct
     5641 aggaaaaatt gcacatattg aaggagagtt catggtttac caggaggaag aggactatcc
     5701 caaggtggca tatgagatta ttgagttttc ccaggagatg agatcctcct accaccagat
     5761 cctccattac ggcaaggaag atatttccgt aatgcaaagc atgttaaaat caatcagatt
     5821 tgcagcagaa aaggcaacag gtggaaatag aaagtctatt gggaaatatc ttgaccatgt
     5881 gtgggaaatt attgagaaaa ctcatgataa cgacttcgat ttatctgttt tggagaatga
     5941 aaagaaagat attttgacta taattgaagg ataatgtcaa aaaaaacaag taaagacttg
     6001 taaattcagt tattcaggag tattattaat ttaaggagtt aataagggaa aggagtgact
     6061 tgaatgaatt ttgatggtaa gcaaatatta tcaacctttg ttaagatgga agaggaagta
     6121 tcaaagtatt acgctgagat ggcaaagaat gcaccagatg aaaaatccaa ggccctattc
     6181 gagagattgt cactggaaga ggacaagcac aataagatgt atgccaagct tctggaaaaa
     6241 cacggcggtg aaatggccag agagttttca gaagaggaga ttgagtacac caagtcactg
     6301 attgatgaga acatcaatgc aaagcatgaa ttcgacaaaa ataagaagtt tggagattca
     6361 ttggagttgg cagaaagaat ggagaaggac ggcatactgt tcgtacatca gatgatggag
     6421 atgtatcctg acatagcaaa caaggaaatg aaggtcatac ttatggaaga gaagaagcac
     6481 cttcaacagg taagggaaag aatgaacttt ggtccgctta ggtcattggg tctataggat
     6541 ttggcaaagc atacggatgg agttttatag ctatcaataa aaacatgccg gtaagatccg
     6601 gcatgttttt gatttgggca gcaaattgct tatttgcggt ttatatgggt atgaaattcc
     6661 tagaatataa agggagatga ttcaattggg aagtgatggt agcttgactg gaaagctgat
     6721 tcttattttg gtgttaaccc tggttaatgc tttttttgct gcgtctgaaa tggcaatggt
     6781 atcagtggac aggataaagc tgtcctccaa ggctgataag ggagacaaaa aagcaaaaat
     6841 gctggttaac atactgaagg agccttccag attcctatcc actatccagg tgggaataac
     6901 ctttgccgga ttcttttcat cggcatcagc tgcagtcagc atatccgcca gactgggtga
     6961 tgcactggac cggatgggga tcccctttgg acaggacctt gcctttatag ggataaccct
     7021 gattctatcc tatgttatgc tggtgtttgg agagctagta ccaaagagaa tcgcattgag
     7081 atatgcagaa aagtttgcca tgcttgctgt taaacccatc aaccttgtcg ctaagataat
     7141 gcatcccttt gtaacctttc tttcaatctc taccaataca gtgatgagaa taatcggtat
     7201 aagccaggag ggtgttgaag ccaaggtaac cctggaggaa attggatcta tggtggaggt
     7261 tgggcatgag caggggttta taaatccggt tgaacgtgag atgatccgaa gtgtgatcaa
     7321 gtttgacgac aagcttgcag aggaaataat gacagcaagg actgaggtct ttgcaataga
     7381 catagacgat cctccagagg agtatatgga ggagttctta aggctcaagc attctagggt
     7441 tccggtctat agggaggatg ttgacaatat aatcggaatt ctgtacctga aggactatct
     7501 tttggaggca tacaggaaag gctttggcaa tattgatata atggatattc tgagacctgc
     7561 atattttgta cctgagagaa agaacatcaa tgacctcttc atggagctaa aatcaaccag
     7621 aaagcacatg gccatactga ttgatgagta tgggggcttt tcagggcttg taaccatgga
     7681 ggacctgatt gaggaaatag ttggagacat tgatgacgaa tttgacctgg atgacccgga
     7741 tgttaaaaaa ctggatgata acctgtattt ggcaaggggt tcagtatcca taaaggaatt
     7801 gaacagcaga acgggactgg agctggatga ggaaagcgag gactatgata cccttgctgg
     7861 cttcctgatc caccagcttg gctacatacc cgatgaccag gagactccag aaataatggt
     7921 ggaccaggtt ctgttcaaga ttcaggaaat tgaggacaag agaataacga aggttctaat
     7981 ccatagcacg tgatatcaga gacgcaatct atcttggttg cgtccttttc atttgcggct
     8041 ttttatggac ttaaggcaat tcatgctgta aaatggaaca aaagagatag gtgaaatgag
     8101 aggataggat cagatgctta gaataagtgg aataagattg gggctggatc aggaggaagg
     8161 gtccatacct tctgaaatat caaaaaaatt gaggataagt gaaaacgaag taaaagcttg
     8221 gcgcatctgg aaaaaatcag ttgatgcaag aaggaagagc aatatttcat ttgtatatac
     8281 agtggatttt aaaacggcca atgataaggc catattgaag aaattcggta aaaagggtgt
     8341 atccctggca agtgtggaga acagggaata tgagaaaaaa acctccatca aggggtccag
     8401 gcctgtgata gtgggaacag gaccagcagg actttttgca ggcctagtgc tttctgagat
     8461 gggatataac ccgatacttc ttgaaagagg caaaaggatc gaggaaaggg taaaggatgt
     8521 aagggagttt tgggaaaccg gaaggctcaa taccgaatca aatgtccaat ttggtgaagg
     8581 gggagcagga accttctctg atggcaagct tacaaccatg acaaatgacc ccagatcaag
     8641 aaaggtattg gaatactttg tagaggcagg tgcaccagag gatatactct atgttaacaa
     8701 gccacacatt ggaacagata tacttagaag agtcgtcata aatataagga agaggattat
     8761 ttccctgggc ggagaggtaa ggtttgagtc caaggtaacg ggttttgaga ttgaggatgg
     8821 caaggtcaag gctgttgtaa taaaccaggg tgaaaggctc cagactgaaa gggttgttct
     8881 tgcacttggg cacagcgcca gagatacctt ccagatgctg tatgaaaatg aatttcatat
     8941 acagcagaaa agcttttcca tgggagtacg aattgaacat ccccaagtcc ttgtggatat
     9001 ttcccaatac ggggaaagcc atgggcatcc aaggctggga gcagcggatt acaagctggc
     9061 tggacatttt gaaaacggcc ggtcggccta caccttctgc atgtgtccag gaggcactgt
     9121 ggtcgctgca gcatcggaaa ggtggatgct ggtgaccaac ggcatgagtg agcatgccag
     9181 aaaca
//
LOCUS       DASRQF010000290         2507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1287653, whole genome shotgun sequence.
ACCESSION   DASRQF010000290 DASRQF010000000
VERSION     DASRQF010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287653"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..1204)
                     /locus_tag="VFD79_07785"
     CDS             complement(20..1204)
                     /locus_tag="VFD79_07785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZH93990.1"
                     /translation="MSTGKIKELAEKYYPEVVELRHYFHMYPELAFEEVKTAAKVAET
                     LERLGIEVQTGVAKTGVVGLIRGGKPGRTVLLRADMDALKVHETADVPYRSRVPGVMH
                     SCGHDGHTAGLLGAAMILNEIKEELSGNVKLVFQPAEEEDGGALPMIEEGVMENPKVD
                     AAFGCHLWGNLLEGKLQFKKGSLMASPDAFDITIIGKGGHAAMPHLSIDPVVMASQVI
                     DQTQAVISRRKSPLDPAVVSFTMVHGGDAYNVIPNEVKLAGTIRTLDHGTRKWIPEAM
                     EAVVKGVTEAGGGSYSFSVKKRFPPLINDDEMTDVAMGSVEKIVGSDNITISDEPNMG
                     GEDFAYFAQEVPSCYFFVGIAKSEEEPVSHHHPEFQWDDRNMILSMEALAQVAVDYLN
                     RD"
     gene            complement(1355..2269)
                     /locus_tag="VFD79_07790"
     CDS             complement(1355..2269)
                     /locus_tag="VFD79_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HZH93991.1"
                     /translation="MRILANDGLDKKAVEVFENENYQVDTDHHTLEELKGIIGDYDVL
                     IVRSATKVFRKLIDAAKGTRLKLVVRAGVGVDNIDVDYAKANGIDVRNTPNSSSNSVA
                     ELVLGHMFGLARHIVSANLTMREGEWNKKHYTGIELQGKTLGIIGFGRIGRALAQKAS
                     ALGMEVVFYDNYIECDERFECLKLDEVLRRADFLTLHVQATEKPMIGENEINIMKDTA
                     IIINTSRGGVVEEDALLEALNSNRLGAAAIDVFTTEPNPNSLLCNHPRVSVTPHIGAA
                     TAEAQERIGQEVVDIVLEIGEKLKTKAS"
BASE COUNT          613 a          702 c          443 g          749 t
ORIGIN      
        1 cattcattaa cccccttaat taatccctat tcaaataatc aaccgccacc tgagccaggg
       61 cttccatgga taggatcatg ttcctgtcat cccactggaa ttccggatgg tggtgactta
      121 ccggctcctc ttcgctcttt gcgattccaa caaagaaata gcaggatggc acctcctgtg
      181 cgaagtatgc aaaatcctcc cctcccatgt taggctcatc tgaaattgta atattatcac
      241 tgccgacaat cttctccaca gagcccatgg ccacatcagt catttcatca tcattgataa
      301 ggggcgggaa cctcttcttt acagaaaagc tgtagcttcc tcctccagct tcggtgaccc
      361 ctttaaccac agcctccatt gcctctggga tccactttct ggtcccatgg tcgagggttc
      421 tgattgtccc ggccagcttc acctcattgg gaataacatt gtatgcatct ccaccgtgga
      481 ccattgtaaa ggatacaaca gctggatcaa gtggactttt tcgtcttgaa ataacggcct
      541 gggtctggtc tatgacctgg gaagccatta caaccgggtc aattgaaagg tggggcattg
      601 cagcatgtcc tcccttgcct atgattgtaa tgtcaaatgc atccggggat gccataaggg
      661 accccttctt gaattggagc ttgccttcca acaggtttcc ccaaaggtgg catccaaagg
      721 cagcatcaac cttggggttt tccataacac cctcttctat cattggaagg gctcccccat
      781 cctcctcttc agcaggctgg aaaaccagct ttacatttcc ggatagttct tcctttatct
      841 cattaagtat cattgcagcc ccgagaaggc ctgcggtgtg tccgtcgtgt ccacaggagt
      901 gcatgactcc aggaaccctt gacctgtatg gaacatctgc cgtctcatgg accttcaagg
      961 cgtccatatc cgccctaagg agtacagtcc ttccaggttt tccccctcta atcagaccga
     1021 caacacccgt tttggctact cctgtctgga cttcaattcc cagcctctcc agggtctctg
     1081 ccaccttggc ggcagttttg acttcctcaa atgccaattc agggtacata tggaaataat
     1141 gcctcagttc cacaacctca gggtaatact tttcagcaag ttccttaatc tttccagtac
     1201 tcataacatc tccctcccct tcttgattca gtatgagctt tatccatttt tccaattaca
     1261 ccataacact ttaaaaggca ataggtaatg aaatattttt tcttattata gcgtttagat
     1321 gaaaatattt ctattattaa acaagggcac ccgcttaact agccttggtc tttaactttt
     1381 ctccgatttc caacactata tcaactacct cctgaccaat cctctcctga gcctcagcag
     1441 tggctgcacc tatatgggga gtcacgctaa cccttggatg gttgcaaagc agggaatttg
     1501 ggttcggttc cgttgtgaac acgtcaattg cggcagcgcc tagcctgtta ctattcaatg
     1561 cttccagaag ggcatcctct tcaaccaccc cacctcttga agtattgatt attattgctg
     1621 tatccttcat gatgttaatt tcgttttcac caatcatggg cttttccgtt gcctgaacgt
     1681 gcagggtcag aaaatctgct cttcttagga cctcatcaag tttaagacat tcaaaccttt
     1741 cgtcgcattc aatatagtta tcgtaaaata ccacttccat tcccagtgct gaggccttct
     1801 gagccaaggc tcttccaatc cttccaaagc caattattcc aagtgttttc ccctgaagct
     1861 caattcccgt ataatgcttc ttgttccact ccccctccct cattgtaagg ttggcactta
     1921 ctatatgtct tgccaatcca aacatgtgac caagaaccag ctctgctacg gaattgctgc
     1981 ttgagttggg tgtgtttcta acatcgattc catttgcctt tgcgtagtcc acatcaatgt
     2041 tgtcaacacc cacccctgcc ctgacaacaa gtttcaatct cgtacccttg gctgcatcaa
     2101 taagcttcct aaacaccttt gttgcagatc ttacaatcag aacatcataa tccccaataa
     2161 tcccctttag ctcttccagt gtatggtggt ctgtatccac ctggtaattt tcattttcaa
     2221 agacctcaac cgcttttttg tccaatccat catttgccaa tattctcatt tgaattcctc
     2281 cctttcgatt ttaataaatc taataaatga atgattccta aagataacct tgatctgcac
     2341 tcccttctta tccaggctta gacaatactc ccatcattgg ccctcctccc ttaatcaaaa
     2401 aaacaaaacc tcgtccacct atagtaaata taagggacgg gggaatatct accgcgttgc
     2461 cacccaaatt gacagattgc tctgccctct caagttcctt tatcgca
//
LOCUS       DASRQF010000291         3901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_796412, whole genome shotgun sequence.
ACCESSION   DASRQF010000291 DASRQF010000000
VERSION     DASRQF010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796412"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..1222)
                     /locus_tag="VFD79_07795"
     CDS             complement(185..1222)
                     /locus_tag="VFD79_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HZH93992.1"
                     /translation="MNIGMKDVISRLEVLLKTPSPTGNTEKVNAFVKELFEEINVPVR
                     FTGKGALIATIEGHDTSRSVTLSGHVDTLGAMVKELKPNGRLKISQLGGYVMDTVEGE
                     HVIVETMDGKTYTGTLVLTKASSHVHGDETKSTERNLENMEIRLDERVSSREDVEGLG
                     IQVGDFVSFDPRTIITPSGFVKSRHLDDKAGVVSLWGIAKYLQDNKIKPNYTTHFFIS
                     NYEEVGHGASAAIPEDTFEFIAIDMAAPGEGQTSDEFSVTICAKDSTGPYDLEIRKRL
                     VELCKKHDINYKVDIYPYYGSDASAALRSGHDLRTGLIGPGVDASHSFERTHQDAIGE
                     TIKLGIAYLTE"
     gene            1365..1775
                     /locus_tag="VFD79_07800"
     CDS             1365..1775
                     /locus_tag="VFD79_07800"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZH93993.1"
                     /translation="MIEVSSGGVVVFGNAILLLKKYNGDWVLPKGRLEKGESIRTAAI
                     REVLEETGVKGEVIEYIGEVNYRYKNLRRNETVYKTVHWYLMRTSSMDCVPQKKEGFV
                     DARFVHINKAKDLLRYTDERKIISKGIELIRQEG"
     gene            1779..2729
                     /gene="whiA"
                     /locus_tag="VFD79_07805"
     CDS             1779..2729
                     /gene="whiA"
                     /locus_tag="VFD79_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HZH93994.1"
                     /translation="MSFSSVTKNELSRIEIDKDCCALAELAALVRMNGTVQITGMQNI
                     NLKFTTENAAIARRIFSILKMMYHSDIEVMVRKNKQLKKNNNYLIIVHDKKISEKVLS
                     DVGYIDKDRSNLFNPRYGVPKNIVASRCCKRSFIRGSFLGGGSMSNPEKTYHLEFVTS
                     HEEHAKSLRDIINSFELNSKIIQRKDNFVVYIKEGEQIVDLLNIIGAHQALLKLEDIR
                     VLKEVRNNINRLVNCETANLGKTISASMRQIESIKTIQRRMGLNKLPDSLREIAEVRL
                     ANPDASLKEIGEMMNPPIGKSGVNHRFRKLEEIADEWGGE"
     gene            2731..2991
                     /locus_tag="VFD79_07810"
     CDS             2731..2991
                     /locus_tag="VFD79_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009431593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HZH93995.1"
                     /translation="MIKKSVTLTNPEGLHARPAALFVKEANKYESSLEIESDSRRVNG
                     KSIIGIMSLGAFQGEEITLSASGPDEEEMVNGLADLLQKSFE"
     gene            3006..3392
                     /locus_tag="VFD79_07815"
     CDS             3006..3392
                     /locus_tag="VFD79_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HZH93996.1"
                     /translation="MKNILVTNNPDVRDKFQDRFQIDYIESGGYIQVLEHIRDLIHKG
                     HQLLTHPLSGSIKPNETPYKSVILSSKKGDLDSSGLTILEDSIMSARKFITNRPTPDW
                     PDHVLEDFRVIDLSLMENVIEKLGHN"
     gene            3473..>3901
                     /locus_tag="VFD79_07820"
     CDS             3473..>3901
                     /locus_tag="VFD79_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015777624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZH93997.1"
                     /translation="MGAKGFAHLHVHTEYSLLDGSIRIKDLIQRTRELGMDSIAITDH
                     GSMFGVVQFYQEARKQGIKPILGSEVYVAMGSHLDKEPKDKSQYHLVLLAENNQGYEN
                     LMKIVSEGYVNGFYYKPRVDHKLLAQYSDGIICLSACLGGE"
BASE COUNT         1181 a          771 c          938 g         1011 t
ORIGIN      
        1 ctcaacttaa ctataaatca gcagaagtgt taccagtgtc ctggttaact ccttaaatca
       61 ttccattgtg aattgcttgt gcccggcgat agaattcctt gaaatttgta atttcaaagg
      121 aattcatcga gagggtatga attgtgaatt gtgatttgtg aattgtgaat tttattttcc
      181 tatcctattc agtaagataa gctattccaa gcttgattgt ttctccgatt gcatcctggt
      241 gggtgcgttc aaaggaatgt gaagcatcga ctccaggccc gatcagaccg gttcttaaat
      301 catggcccga ccttagggct gccgaggcat cggacccgta gtaggggtag atatcaacct
      361 tgtagttgat gtcatgcttt ttgcagagtt ctacaagcct cttcctaatc tccagatcat
      421 agggtccggt actgtccttg gcacagattg taactgagaa ctcatctgat gtctggccct
      481 ctcccggggc tgccatgtca atggcgataa actcgaatgt gtcctcggga attgcagctg
      541 atgcaccgtg tccaacctcc tcgtagttgc tgataaagaa gtgggtggtg tagttgggct
      601 ttatcttatt gtcctgaagg tacttggcta taccccaaag gctaacaaca ccggccttgt
      661 catccaggtg tctggatttc acaaagccag agggtgtaat gatggtcctt gggtcaaagg
      721 acacaaaatc accaacctgg attccaagcc cctccacatc ctccctggag gaaacccttt
      781 catccaggcg gatctccata ttctcaaggt ttctttcagt ggattttgtc tcatctccat
      841 ggacatggct agatgccttg gtaaggacca gggtacctgt gtaggtcttc ccatccattg
      901 tttcaacgat tacatgctca ccttcaacag tatccataac ataacctcca agctgggata
      961 tctttagtct accgttgggt ttcagctcct tgaccattgc gcccagtgta tccacgtggc
     1021 ctgacagggt gacagacctg ctggtgtcat gaccctcaat tgtggctata agtgctccct
     1081 ttcccgtgaa cctgacagga acattgattt cttcgaataa ttcctttaca aaagcgttta
     1141 ctttttcagt atttccagtg gggcttgggg tcttgaggag cacctcaagg cgactgatta
     1201 catccttcat tcctatgttc atagcttgcc tcctgacctc ttagtcttct atacctctta
     1261 ttatacagca agagttcaaa tttgtgaaaa tgtttacaaa aacctagtaa aatagtgtat
     1321 aattaatata atgtgtagta atgtagatta ttgggagtgg tattatgata gaggtcagct
     1381 caggaggagt tgttgtattt ggcaatgcta tcttgctgtt aaaaaaatat aacggagact
     1441 gggtactacc aaagggtaga ctggaaaagg gagagagcat aaggactgca gccataaggg
     1501 aagtgctgga ggagacggga gtaaaagggg aagtcatcga gtatataggt gaagtcaact
     1561 acaggtacaa gaatcttaga agaaacgaga cggtatacaa aacagtacac tggtatctaa
     1621 tgcgaaccag ctccatggac tgcgttcctc aaaagaagga aggctttgtg gatgcaaggt
     1681 ttgtccatat aaacaaagct aaggaccttt tgagatatac tgatgaaaga aaaattatat
     1741 ccaaggggat agaattaatt aggcaggagg gatgatggat gtcgttttca tccgttacca
     1801 aaaacgagtt atccaggata gaaatagaca aggactgctg cgccctggca gagcttgccg
     1861 ccttggtgag aatgaacgga acagttcaga ttacaggaat gcagaacatc aaccttaagt
     1921 tcaccacaga aaatgcagca atagccagaa ggatcttttc aatactaaag atgatgtatc
     1981 actcagatat tgaggtcatg gtgcgaaaga acaagcagct taagaaaaac aacaactatc
     2041 ttataatagt ccatgacaaa aaaatatcag agaaagtcct ttccgatgtg ggctacattg
     2101 acaaggacag gagcaatctt ttcaatccaa ggtatggtgt tcccaagaat atcgtggcat
     2161 cgagatgctg caagaggagc ttcataaggg gatcctttct tggtgggggc tcaatgagca
     2221 atcctgagaa gacatatcac cttgaatttg tcacaagcca cgaggagcac gccaagagtc
     2281 tcagggacat aatcaattca tttgagctga attcaaagat tatccaaagg aaggataatt
     2341 ttgtggtcta tatcaaggag ggggagcaga ttgtggacct tctcaatata ataggagccc
     2401 accaggcatt attgaagctt gaggacataa gggttctaaa ggaagtaaga aacaacatca
     2461 acagactggt caattgcgaa actgcaaatc tcggtaagac aatcagtgca tccatgagac
     2521 agattgaaag cataaagacc atccagagaa gaatgggtct gaataaatta ccagacagcc
     2581 ttagggagat tgcagaggta agattggcaa atcctgatgc cagccttaag gaaattggtg
     2641 aaatgatgaa tccacctatc ggcaagtccg gggtgaacca tagattccga aaactggaag
     2701 aaatagcaga tgaatgggga ggagaatagt atgataaaga aatcggttac acttacaaat
     2761 ccagagggcc ttcatgcaag acctgcagcc ctttttgtca aggaagcaaa taaatatgaa
     2821 agtagtcttg aaattgagtc tgatagcaga agggtcaatg gaaagagtat aatagggata
     2881 atgtccctgg gagcatttca gggggaagaa ataaccctat cggcaagtgg tcctgacgag
     2941 gaagaaatgg ttaatggtct agcagacctt cttcaaaaga gctttgagta ggcagaggag
     3001 gagctatgaa aaacatattg gttaccaata atccggacgt aagggacaag ttccaggata
     3061 ggtttcagat tgactacatt gaaagcggtg gttacatcca ggttcttgag catatcaggg
     3121 acttgatcca caagggccat cagcttttga cccatcccct ttcagggagt ataaagccaa
     3181 atgaaacgcc atataaaagc gtaatcctgt catcaaaaaa gggtgacctt gactcatcag
     3241 gactgacaat ccttgaggac agcatcatgt cagccagaaa gtttataaca aacagaccta
     3301 cacctgactg gccggaccac gtactggagg atttcagggt gatagatttg tcattgatgg
     3361 aaaatgtaat tgaaaaacta ggtcataatt aggtcttctc actggagaag gctttttcat
     3421 ttacccctgt tttgtggtaa aatctatgta tatctatacg aggagtgagt ttatgggagc
     3481 aaagggattt gcacaccttc atgttcatac tgaatacagc ctgctggacg gatcaataag
     3541 gataaaggac ctgatccaac ggacaaggga actgggaatg gattccatag ccatcactga
     3601 ccatggttca atgtttggag tggtccagtt ctatcaggag gctaggaagc agggaattaa
     3661 gccgattcta ggttccgagg tttatgttgc catgggaagc cacctggata aggagcccaa
     3721 ggacaagagc cagtatcacc tggtactgct tgctgaaaac aatcagggct acgagaacct
     3781 tatgaaaatt gtttcggagg gctatgtcaa tggtttctac tataagccaa gagtggacca
     3841 caagcttctt gcccagtaca gcgacggaat catatgcctt tcagcatgcc ttggtggaga
     3901 g
//
LOCUS       DASRQF010000292         2750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_65053, whole genome shotgun sequence.
ACCESSION   DASRQF010000292 DASRQF010000000
VERSION     DASRQF010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65053"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VFD79_07825"
     CDS             <1..444
                     /locus_tag="VFD79_07825"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HZH93998.1"
                     /translation="ENYYREQAEKNKGNALEKVFLNLADDEKKHAELVRKYAEAVDYS
                     LDEDNAFVEFENVFKNESDFKVDVKVDPNQLDAYRLALKKEQESIDLYKKMKDEADSE
                     KGKKLFDYLIRQEEYHYRIFDDMVNHLRKAEEWVEDARFGKRDTY"
     gene            492..1058
                     /locus_tag="VFD79_07830"
     CDS             492..1058
                     /locus_tag="VFD79_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HZH93999.1"
                     /translation="MKSLIVVDCQYDFIDGSLACENSEQAVYRIIDYINKNDIRVHYS
                     MDWHSMDNRSFQINGGIWPVHCVQGEKGARLHDGFYRLVDHEKNRPNEENVFYKGMDD
                     EVEEYSAYNAKNSRDEILKDVLDGGVVVSGIASEFCVRETVLALLAGGYRVEILEEGL
                     GYVDSSEHKKNLKELEKKGVRIIQQQQG"
     gene            1101..1424
                     /locus_tag="VFD79_07835"
     CDS             1101..1424
                     /locus_tag="VFD79_07835"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZH94000.1"
                     /translation="MNKDKVVYLNSEEDLEELSKKDIHILYFSSENCNVCHSVFPKLV
                     EITFELDIEIGRIDVNKNVKLAGQHLIFSIPTILVFHEGREVARESRFINFDKLERTL
                     NILLS"
     gene            1534..1992
                     /locus_tag="VFD79_07840"
     CDS             1534..1992
                     /locus_tag="VFD79_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZH94001.1"
                     /translation="MYIKDIMTRDVITVTMEDNVEKCASLLIKHNLSGLPVLDEAGKL
                     VGIVTEGDLIRRASRIQGPAVLEVLGGLIYLDSPKKFIDELKNSMGQKAGDVMTKKVV
                     TVDPDQTIEEGATLLVEKKVKRLPVVNKEGELVGIVSRRDIMSYLFPEQE"
     gene            2020..>2750
                     /locus_tag="VFD79_07845"
     CDS             2020..>2750
                     /locus_tag="VFD79_07845"
                     /inference="COORDINATES: protein motif:HMM:NF015060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HZH94002.1"
                     /translation="MARKSEGILTGYMALAMYLVMVATNALANILPINGITTGEVSDN
                     LPNLFAPAGITFSIWGVIYILLGIYSIYGLVYINRGYGDNKALKRINQLYIVSSIANC
                     IWIYNWHHGNVGLTLVLMLVILVSLAYIVLTLRSVATSGDRMLWARLPFSVYFGWITI
                     ATIANVTAYLVDIGWDGFGISEQMWTIVVLFVGLLIGGSTIWRLKDKAYGAVMVWAYM
                     GILLKHISQDGFDGSYPGVIVTAGIA"
BASE COUNT          875 a          437 c          717 g          721 t
ORIGIN      
        1 gagaattatt acagagaaca ggcagagaag aacaagggca atgctcttga gaaggtattt
       61 ctgaatctag ccgatgatga gaaaaagcat gctgaactgg taaggaaata tgctgaagct
      121 gttgattaca gtcttgacga agacaatgca tttgtcgagt ttgagaatgt attcaaaaat
      181 gaatcagatt tcaaggttga tgtgaaggtt gatcccaatc agcttgatgc gtatagatta
      241 gcccttaaga aggagcagga aagtatagac ctttacaaga agatgaagga cgaggcagat
      301 tcagagaaag ggaagaaact gtttgactat cttatcagac aggaagaata ccactacaga
      361 atttttgacg acatggtaaa tcaccttaga aaagctgaag agtgggttga ggatgccaga
      421 ttcggaaaaa gagatactta ttagactaaa ggcagccttg tggctgcctt gctccattca
      481 gaggtgaaaa aatgaaaagt ttgattgtag tggactgcca gtatgacttc atagatggat
      541 cattggcctg cgaaaactca gaacaggccg tatatagaat aattgactat ataaataaga
      601 atgatatcag ggtacactat tcaatggatt ggcatagcat ggacaacagg agcttccaga
      661 taaatggagg gatctggccg gtgcattgtg tacagggaga aaagggcgca aggttacatg
      721 atggttttta cagacttgtg gaccatgaga aaaacagacc taatgaagaa aatgttttct
      781 ataagggtat ggatgatgaa gttgaggagt actcagccta caatgcaaaa aactctcgtg
      841 atgaaattct aaaggatgtt ttggatggtg gggttgtggt ttccggcata gcaagtgagt
      901 tctgtgtcag ggagactgtt cttgccctct tggctggtgg atacagggtt gaaatccttg
      961 aggaaggctt gggatatgtt gattccagtg aacacaagaa gaatcttaag gagcttgaaa
     1021 agaagggggt caggataatc cagcaacagc aaggataaat gtaatggatc agagaaaatt
     1081 aataattggg gaggacaagc ttgaataaag acaaggtagt atatttaaac tcggaggaag
     1141 accttgagga gctttcaaag aaggatatcc atatcctcta cttcagctcg gaaaactgta
     1201 atgtttgtca ttcggttttc cctaaactgg tggagattac atttgaactg gacattgaaa
     1261 tcggaaggat cgatgtcaac aaaaacgtaa agctggccgg tcagcacttg atattttcaa
     1321 taccgacaat acttgtcttc catgagggca gggaagtggc cagggaaagt cggtttatca
     1381 actttgataa acttgagaga acccttaata tacttttatc ctagaagatt aaaaaaaggc
     1441 attcatcagc tgatgattgc cttttttgtt caaaatacca ataattgggt atcatccact
     1501 tagaaggaaa atcaagaaat tgggggagtt catatgtata tcaaggatat catgacaagg
     1561 gatgtaataa cagttacaat ggaagacaat gtggagaaat gtgcttcact tcttatcaag
     1621 cacaacttga gcggtcttcc ggtacttgat gaagccggaa agcttgtggg catagtcaca
     1681 gagggggacc ttataaggag ggcatcaagg atacagggac cagctgtcct agaggtcctg
     1741 ggagggctga tatacctgga tagtccaaaa aaattcattg atgagcttaa gaactcaatg
     1801 ggtcagaagg caggagatgt aatgaccaag aaggttgtaa cagtagatcc tgaccagacc
     1861 attgaagagg gagcgactct gttggtggag aagaaggtca agagattgcc tgttgtgaat
     1921 aaggagggag aacttgttgg aatagtatca agaagagata ttatgagtta ccttttcccg
     1981 gagcaggagt aatgaggcta aatgtaaagg gggagaagca tggctagaaa aagtgaagga
     2041 attttaaccg ggtacatggc attggcaatg tatcttgtaa tggttgctac aaatgctcta
     2101 gcaaacatcc tgcccataaa tggaatcacc acaggtgagg tttcagacaa tctgccgaac
     2161 ctgtttgcgc ccgcagggat tacattttca atatggggtg taatatacat cctgttggga
     2221 atctattcaa tttatggcct tgtgtacata aaccggggat acggagacaa caaagcccta
     2281 aagaggatca accagttata cattgtttca tcaattgcaa actgcatatg gatctataac
     2341 tggcaccacg gaaatgtcgg cttgaccctt gttcttatgc tggtaatttt ggtttcgttg
     2401 gcatacattg tactgacctt aaggtcggtt gccacatcag gagataggat gctttgggca
     2461 aggcttccct tcagtgtgta ttttgggtgg attactatag caaccatagc caatgtaact
     2521 gcatatcttg tagacattgg ctgggacggc tttggaattt cagaacagat gtggaccata
     2581 gtggtcttgt ttgtagggct gcttattggt ggaagcacga tatggagact taaggacaag
     2641 gcttacggtg ccgtcatggt gtgggcatat atgggaatac tccttaagca catatcccag
     2701 gatggttttg atggaagtta tcccggtgta atagttacag caggaattgc
//
LOCUS       DASRQF010000293         7358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_421228, whole genome shotgun sequence.
ACCESSION   DASRQF010000293 DASRQF010000000
VERSION     DASRQF010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 7358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7358
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421228"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VFD79_07850"
     CDS             complement(<1..244)
                     /locus_tag="VFD79_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004838558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HZH94003.1"
                     /translation="MALRRVRLDGDPILRKKSREVTEIDDRILQLLDDMVETMRHENG
                     IGLAAPQVGILKRVITIDVGEEPIKAINPVIQEEEGL"
     gene            complement(269..2713)
                     /gene="priA"
                     /locus_tag="VFD79_07855"
     CDS             complement(269..2713)
                     /gene="priA"
                     /locus_tag="VFD79_07855"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HZH94004.1"
                     /translation="MSFADIIIDHKSTSVDRRFTYSIPDELLDDAKVGVRVVVPFGRY
                     NKPTMGIIVEMKEKLDSNIKTKDIVRILDDKPLISEKRLELAKWIKDQYISTGFESMQ
                     PILPPGDFRDISTIVVLREEFKGFHLVSDKGRKILSDLSEAGKIEYSRLRTMYNDGGF
                     NREISALEELGAIRLVLQVRKNTTTRVERFIVANTGVSYQQGIEIAGKGAKKQLEIWR
                     FISAAKEADLRDLLLDFNTSLSVVKAMEKKNLIKIETRLVDEKPISEKISEYKRIELN
                     KEQREVYDGIVGSQDNLFLIHGITGSGKTEIYLQLVEDMLRKGKTSIILVPEISLTPQ
                     TTDRFVGRFGNQVAILHSKLTQLERFDQWRRIKSGEYNIVIGARSALFAPFNNLGLIV
                     IDEEHENTYKSSNNPKYSTVEVAKKISELENAKLVLGTATPSVETYFKAKNGEFHLFE
                     LKNRATRNTLPSINIVDMRRELEEGNRSIFSRELYYEMEKTLVEGNQLILFLNRRGYT
                     GFITCRSCGYVSKCNNCDISLTYHRSTNRLRCHYCGETHAPPRSCPSCGSNYFKSFGI
                     GTERVEEEVKKAFPNARISRMDSDNIKTRDDYETALDSIKNKEIDILIGTQMISKGLD
                     FPSVTLVGIIAADTTLNLPDFRAPERTFQLVTQVSGRAGRGDVAGKVILQTYNPDHYS
                     ITTAMNNDYKEFFRNELELRKEFGYPPYREIATITLFGKNHQDVKESMEKSSAMLVKL
                     VREADLGEVDILGPNPAPMEKINNNYRWQILLKFGYNNHKDIRNIIRRVYTTGKGNFI
                     KEDVKTSIDINPNSIL"
     gene            complement(2751..3956)
                     /gene="coaBC"
                     /locus_tag="VFD79_07860"
     CDS             complement(2751..3956)
                     /gene="coaBC"
                     /locus_tag="VFD79_07860"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005956868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HZH94005.1"
                     /translation="MLKDKHILLGVTGGIAAYKSADLVSRLIKAGAKVDVVMTRGATE
                     FVSPLTFQTMSQSKVHFDLFGLESNMEVEHISLAQRPDAILIAPATANTIGKIANGIA
                     DNMLTTVIMASTARIIFAPAMNTNMYLNPITQGNINKLKELGHLFLDTGKGRLACGDV
                     GAGKMAEPADIVEYLEYFFTEKDLLGKKIVVTAGPTVEAMDPVRFISNHSSGKMGYAI
                     ARNAVMRGAEVVLVSGPVNLKPPTGVRLVQIESTKDMLDAVKDDFDTCDVLIKAAAPS
                     DFRPSNYHDRKIKKRENGSENMSVELTRNPDIAAHFGKIKEKRIMVGFAAETNNLEEY
                     ALMKMKEKNFDFIVANDVTQEGAGFKGDTNIVSVIDRSGKREDLPMMPKTDLASVILD
                     KVKSLLLNV"
     gene            complement(3965..4171)
                     /gene="rpoZ"
                     /locus_tag="VFD79_07865"
     CDS             complement(3965..4171)
                     /gene="rpoZ"
                     /locus_tag="VFD79_07865"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HZH94006.1"
                     /translation="MNDKIKENSLPGESRYTLVMVAARRARQLVEGSSPRVDTKANKP
                     VTIALDEIAEGKVEFVNPSAGSFK"
     gene            complement(4161..4787)
                     /gene="gmk"
                     /locus_tag="VFD79_07870"
     CDS             complement(4161..4787)
                     /gene="gmk"
                     /locus_tag="VFD79_07870"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HZH94007.1"
                     /translation="MAKGFLLVLSGPSGSGKGTVSQALMKKRDDIVFSVSATTRKPRA
                     TEVDGENYFFYEKDLFMDMVEKGEFLEHAFVHTNYYGTPKKFVFDQIDKGEIVLLEID
                     VQGALQIKKNYKEAVFIFLLPPTMDELRSRIVKRGTESDEDIDTRFANAFKELDFVGE
                     YDFFVVNDKVEAAVADIEAIIHSEKLRVKRHSGIKEQVTRKGGNGNER"
     gene            complement(4810..5067)
                     /locus_tag="VFD79_07875"
     CDS             complement(4810..5067)
                     /locus_tag="VFD79_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_570902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HZH94008.1"
                     /translation="MLVYFINVGFGNVVASNKVVSVVSPESAPIKRLVQESKEHGTLI
                     DATYGRRTRAVIVMDSGHIVLSPLQPETIAHRLHAKEEVHD"
     gene            complement(5079..5960)
                     /locus_tag="VFD79_07880"
     CDS             complement(5079..5960)
                     /locus_tag="VFD79_07880"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HZH94009.1"
                     /translation="MIRSMTGYGKGEVENQLYKLKIELKSVNHRYLDINVKLPRYLIY
                     LEEGIKKLVKEKINRGKVDVFVNFDFADASSLDVKVDIPLAKSFKKALEELKDELEIE
                     DSIRLNNILSVSDVIKTEKKELDEDLVWETIREATGIALDKILQMREYEGEQLRNDIY
                     TKLDNIEIISDRIEKRAPLVVDEYRGKLNDRINSILEDGTIVDPDRLAVEVAIYADKS
                     SIDEELTRLKSHVLQLRSILSEEDAIGRKLDFLIQEFNREVNTIGSKSSDTEIVKTVV
                     EMKSEIEKIREQVQNIE"
     gene            6192..>7358
                     /locus_tag="VFD79_07885"
     CDS             6192..>7358
                     /locus_tag="VFD79_07885"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HZH94010.1"
                     /translation="MSFDGVVTRSVVSELNRILVGGRIDKIYQQEKDELLLQIYSKGK
                     NHRLLMSASSNNPRLYITDSSKSNPLSPPMFCMLLRKHLSGSIILDIRQNHLDRVVIF
                     TITGKDELGHPTERELIIEIMGKHSNIILINKESKRIIDSIKRIPAEISRVRQILPGL
                     SYSYIESENKEDPLELNQDIFNQQLDKSNGSQKIFKFLYQSFTGLSPLAGREICSLSG
                     LEPDRVVLSLENDHKERLYKGMNEFMTRVRDNDFRPVYVLGDDGLDLVAFYALPLTQY
                     SGLEQVYTDSVSQMLDFVYKSKDTSDRVSQKSASLRKLLGTKLERAQNKLSKQKEELL
                     DSRDREKYKIYADLLQSNLHRINRGSDHVVLENFYDENMENLRVPLDPKLSPAEN"
BASE COUNT         1947 a         1743 c         1264 g         2404 t
ORIGIN      
        1 ccaaaccttc ttcctcctgg attaccgggt tgatggcctt aatgggttcc tcccccacat
       61 cgattgttat aactctcttc agaatgccta cctgtggtgc tgcaagacca atcccattct
      121 catgtctcat ggtttccacc atatcatcca ggagttgaag tatcctatca tcgatttctg
      181 tcacctctct ggattttttc cttaatattg gatccccatc caacctaact ctacgcaatg
      241 ccattatagt cctccttgtt aactcgtttt acagtatgct gttcggattt atgtctatac
      301 ttgtttttac atcctctttt ataaaattac ccttaccagt agtatatacc ctacgtatga
      361 tattccttat atctttatga ttattgtaac caaattttaa taaaatctgc cacctgtagt
      421 tattgttaat cttctccatt ggtgccgggt ttgggccaag aatatcaact tcccctaaat
      481 ctgcttccct aaccagttta actagcatgg ctgatgattt ctccattgac tccttcacat
      541 cctgatgatt ctttccaaaa agtgtaatag tagcgatttc cctgtaagga gggtacccaa
      601 actcctttct tagctccaat tcatttcgaa agaactcctt gtaatcattg ttcatggcag
      661 tagtaatgct gtagtgatca ggattgtagg tctgtagaat aacctttcca gccacatcac
      721 cccttccggc tctacccgaa acctgtgtca caagctggaa tgttctctca ggagccctga
      781 agtcaggaag gtttaaggtc gtatctgctg caatgattcc aacaagggta acagagggaa
      841 aatcaagccc ctttgaaatc atctgggttc caatcaatat gtcgatttcc ttgtttttga
      901 tggaatcaag ggccgtttca tagtcatccc tggttttgat gttatcgctg tccattcgtg
      961 atatccttgc atttggaaat gcttttttca cttcctcttc cactctttct gtccctattc
     1021 caaagctttt gaagtagttg cttccacatg atgggcagga tcttggaggg gcatgtgttt
     1081 ccccgcaata gtgacatctg agtctgttgg tggacctgtg ataggtaaga ctaatgtcgc
     1141 aattgttgca tttggataca tagccacagc ttctgcaggt tatgaagcct gtgtaacccc
     1201 ttctattgag aaaaagaatc aattgattcc cttcaacaag ggtcttctcc atctcgtagt
     1261 acagttccct gctgaatatg gatcggttac cctcctccag ctccctcctc atgtcaacaa
     1321 tattgattga tggcaatgtg ttccttgtgg ccctattctt aagttcaaac agatggaact
     1381 ccccattctt tgccttgaaa taggtctcca cagagggtgt cgcagtccct agaacaagct
     1441 ttgcattctc cagctcgctg attttcttgg caacctccac tgtgctgtat tttggattgt
     1501 tggaggactt gtaggtattc tcatgttctt catctattac tatcagtccc agattgttga
     1561 aaggtgcgaa caaagcagat ctcgcaccaa taacaatatt atattcgcca gatttgattc
     1621 ttctccactg gtcaaatctt tccagttgtg taagcttgct gtgaagtatg gctacctgat
     1681 tgccaaacct tccaacaaac ctatctgttg tctgaggtgt gagtgatatt tcaggaacaa
     1741 gtatgattga agtcttgcct tttctgagca tatcctccac aagctgcagg tagatttcag
     1801 tcttgccgct accggttatt ccgtgaatga gaaataaatt gtcctgtgac cctacaatac
     1861 cgtcataaac ctctctttgt tccttattga gttctattct tttgtattcc gaaattttct
     1921 cggatatggg cttttcgtcc accaacctgg tttctatttt tatgaggttc ttcttttcca
     1981 tggccttaac cactgacagg ctggtattga aatccaataa aaggtctctc aaatcagctt
     2041 ctttagcagc gctgataaag cgccagattt caagttgttt ttttgcaccc ttgcctgcta
     2101 tttcaatgcc ttgctggtac gaaacaccag tgtttgcaac aatgaatcgt tcaaccctgg
     2161 ttgtggtgtt cttcctgact tgaagaacca gtcttattgc ccctaattct tctagggcag
     2221 atatttccct gttgaaccca ccatcattat acatggttct taggcgggag tactcaattt
     2281 tcccagcctc tgacaagtca ctcaggattt tccttccctt gtcagacact aggtgaaacc
     2341 ccttgaattc ctcccttaaa accactatgg tacttatatc tctgaagtct ccgggtggca
     2401 atattggttg catggattca aaccctgtac taatgtattg gtccttgatc catttagcca
     2461 actctagtct tttctctgaa atcaagggtt tgtcgtccag tattctaaca atgtccttgg
     2521 ttttaatatt ggaatccagt ttttctttca tttcaacaat tatacccatg gtaggtttgt
     2581 tgtatcttcc aaagggaacc acaaccctaa ctcccacctt ggcatcatca aggagctcat
     2641 ctggtatact atatgtgaac cttctatcca cgctggtgga cttatgatcg ataatgatat
     2701 ctgcaaagct catccaataa cccctttcag acgctacacc ttaatactcc ttatacattt
     2761 aacaaaaggg actttacctt gtccaatatt acagaggcca aatcagtctt tggcatcatt
     2821 ggtaaatctt cccttttgcc tgatctgtca atgacactta ctatattggt atctccttta
     2881 aaccctgccc cctcctgtgt tacgtcattg gcaactatga aatcaaagtt cttctccttc
     2941 attttcatca aggcatattc ctcaagattg ttggtttctg ctgcaaatcc aaccattatc
     3001 ctcttctcct tgatttttcc gaagtgagca gcaatgtcag gatttcttgt tagttcaact
     3061 gacatgttct cagagccgtt ctcccgcttc ttaattttcc tatcatgata gttcgatggc
     3121 ctaaaatcag aaggggccgc tgctttaatc aaaacgtcac aggtgtcaaa atcgtccttt
     3181 accgcatcca acatatcctt tgttgattca atttgcacca acctaacacc tgttggtggt
     3241 ttaaggttta ccggtccaga cacaagtaca acttccgcac ctctcattac tgcattcctg
     3301 gctatggcat aacccatttt cccgctggag tggttggaga tgaaccttac aggatccatg
     3361 gcttcaacag taggtcctgc agttacaact atctttttgc ccaagaggtc cttttctgta
     3421 aaaaaatatt caagatattc aacaatatca gcaggttctg ccatctttcc tgcacctaca
     3481 tccccgcaag caagccttcc cttgcctgtg tccaagaata ggtggcccag ttccttcagt
     3541 ttgttgatat ttccttgagt tatgggattt agatacatgt tggtattcat tgcaggagca
     3601 aagattattc tggcagttga agccattatt accgtagtta acatgttatc tgcaatccca
     3661 tttgcaatct tgccgattgt attggcagtg gctggtgcta taagaattgc atctggtctc
     3721 tgtgcaaggg aaatgtgttc aacctccatg ttgctttcca atccgaacaa gtcaaaatgc
     3781 accttgcttt gtgacatggt ctgaaaagtc aggggactta caaattcagt tgctcccctt
     3841 gtcattacaa catccacctt tgcaccagcc tttatcagtc tactaaccag gtcagctgat
     3901 ttatacgctg ctattccacc tgttacaccc aatagtatgt gcttgtcctt taacatgtcc
     3961 tcacctactt aaagcttcca gcagaaggat taacaaactc cacctttcct tcagctatct
     4021 catccagagc aattgtaaca ggcttgttgg cctttgtatc caccctaggg gatgaacctt
     4081 ccaccaattg tcttgccctt ctcgctgcaa ccattacaag tgtatatcta ctttcacctg
     4141 gcaagctgtt ttcctttatc ttatcgttca ttgccattac ctccttttct agttacctgt
     4201 tccttgattc cactatgtct ttttacgcga agtttttctg agtgaattat ggcttcaata
     4261 tctgctactg cagcttcaac cttgtcattt acaacaaaaa aatcatactc tccaacaaaa
     4321 tcaagctcct tgaatgcatt tgcaaatctg gtatctatat cctcatcgga ttctgttccc
     4381 cttttgacaa tcctacttct cagctcatcc atggttggtg ggagaaggaa aataaatacc
     4441 gcttccttat aattcttttt gatttgcagg gctccttgaa cgtcaatttc aagtaacact
     4501 atctcgcctt tgtctatctg atcaaaaaca aacttttttg gagttccgta ataatttgta
     4561 tgaacgaatg catgttctaa gaactcaccc ttttcgacca tatccataaa taggtccttt
     4621 tcatagaaaa agtagttctc cccatccact tcagttgccc tgggctttct tgtggtagcc
     4681 gataccgaga acacaatgtc atccctcttt ttcataagag cctgacttac agtacccttt
     4741 ccactacctg aaggtcctga aagaaccaat aaaaaacctt ttgccataac tttccaccat
     4801 ccttctcaat taatcatgca cttcctcttt ggcgtggagt ctatgtgcaa tagtctccgg
     4861 ctgcaaaggg gacaatacaa tatgtccact atccataacg ataactgccc tggtccttct
     4921 tccgtatgtt gcatcaataa gtgttccatg ttccttggat tcctgtacaa gcctctttat
     4981 gggtgcagat tccggactga caacagaaac aaccttattg gaagcaacaa cattaccaaa
     5041 gccaacatta atgaaataaa ctaacatttc aatgcctcct actcgatatt ctgaacctgt
     5101 tctctgattt tttcaatctc tgatttcatc tcaaccactg tcttaactat ttcagtatca
     5161 cttgatttac tgccaatggt atttacttcc ctgttgaact cctgaatcag gaagtctagt
     5221 ttcctgccaa tagcatcctc ctctgataat attgacctaa gttgaagaac atgactcttt
     5281 agccttgtca gttcctcatc aatgctagat ttatcagcat atattgcaac ttccacagca
     5341 agtctatcag gatcaacaat agttccatcc tcaagaattg aattgatcct atcattcaat
     5401 ttcccccgat actcatctac aaccagaggg gccctttttt caatcctgtc acttatgatc
     5461 tctatgttat ccaactttgt gtagatgtca ttccttagtt gctctccctc atactccctc
     5521 atctgcagga tcttatccaa agcgattcct gtggcttctc ttatggtttc ccaaacaagg
     5581 tcctcgtcga gttccttctt ctcagttttt ataacatcag atactgacag gatattgttc
     5641 agcctgatgc tatcctctat ttccaactcg tccttcaact cctccaaagc cttcttgaag
     5701 ctttttgcca atggtatgtc cacctttaca tccagggaag aagcatctgc gaaatcaaaa
     5761 tttacaaaca catcgacctt ccctctgttg attttttcct tgaccagttt ttttattcct
     5821 tcctccaaat aaatcaagta tctaggtaat ttgacattta tgtccaaata tctgtgattt
     5881 accgacttta gttcaatttt cagcttatac agctggttct caacctctcc ctttccatag
     5941 ccagtcatac ttcttatcaa aaaatcccct cccttatcac tcataaattt attgttattt
     6001 caatatatgt tggaattata agcctgacaa tctatgagtt atagactatt atacatctat
     6061 gaatataaat atctacactt tgttacaaat tgttaatatg tttggtgtaa atgtagtttt
     6121 gttttgcaaa ttcttcatcc cttgtattta tgatatactt attagacaag aaaatttatg
     6181 gaggttttat aatgtcattt gacggtgtag ttaccaggtc agttgtatct gaattgaata
     6241 gaatcttggt cggtggtagg attgataaaa tctaccagca agaaaaggat gaattgcttt
     6301 tacaaatata cagcaaagga aagaaccaca gacttttaat gtctgccagc agcaataatc
     6361 caagacttta tatcaccgat tcatcaaaat caaacccctt gtcgccaccc atgttctgta
     6421 tgctactaag aaagcacctt tctggaagca taatcctgga tattaggcaa aatcatctgg
     6481 atagggttgt catattcaca ataaccggca aggatgaact tggacatccc actgaacggg
     6541 aattgattat cgaaatcatg gggaaacata gcaatataat tcttataaac aaggaatcaa
     6601 aaagaattat agactccatc aagaggattc cagcagaaat ttccagagta aggcagatac
     6661 tccccggatt gtcatattca tatatcgagt ctgaaaacaa ggaggatccc ctggagctca
     6721 atcaagatat ttttaaccag caacttgata aaagcaatgg tagtcagaaa atattcaaat
     6781 tcctgtatca gagctttact gggctaagcc ctttggctgg tagggagata tgtagcctct
     6841 caggccttga acctgacaga gttgtcctaa gccttgaaaa tgaccacaag gaaagacttt
     6901 acaaaggtat gaatgaattc atgactagag ttagggataa tgactttagg ccagtttatg
     6961 ttctaggtga tgatggcctt gaccttgtgg cattttatgc ccttcctcta actcaatata
     7021 gtgggttaga acaggtctac actgacagtg tatcccagat gttggatttt gtatacaagt
     7081 ccaaggatac ctctgacagg gtcagccaaa aatctgcctc ccttaggaag cttctgggta
     7141 ccaaactgga aagggctcaa aacaaactct ccaagcaaaa agaggagctt ttggatagca
     7201 gggataggga aaaatacaag atttacgcag atctactcca atccaacctg cacaggatca
     7261 atcgaggcag cgaccatgtt gtccttgaaa atttttatga cgagaatatg gagaacctaa
     7321 gggttccact tgacccaaag cttagtcctg ctgaaaat
//
LOCUS       DASRQF010000294         8915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1195699, whole genome shotgun sequence.
ACCESSION   DASRQF010000294 DASRQF010000000
VERSION     DASRQF010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 8915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8915
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195699"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VFD79_07890"
     CDS             <1..364
                     /locus_tag="VFD79_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_except=(pos:101..103,aa:Sec)
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HZH94011.1"
                     /translation="GNGTAVASSKKFANEFSKELIADGVDAVILTSTUGTCTRCGATM
                     VKEIERAGIPVVHICTVVPISLTVGANRIVPAIAIPHPLGNPSMDKVEEKALRRKIVE
                     KSLVALETEVTEQTVFDK"
     gene            438..1976
                     /gene="grdC"
                     /locus_tag="VFD79_07895"
     CDS             438..1976
                     /gene="grdC"
                     /locus_tag="VFD79_07895"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009372447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit beta"
                     /protein_id="HZH94012.1"
                     /translation="MSYSVVKGTSYVLVHAPDMVLHNGTTQTTEKHLNPNSEYLKNLP
                     NSLRTFDEAVNYLPNQVYIGNNKPADLRKDPQPWVGKNVDGASRNGKLGEIMPQDEFI
                     GLIKIVDAFELVKLTKEFTADVKKKLNEHPLVKDELVAKLKEGEDIADIQKLINDQGA
                     EPLYMNNEVIGCIKRAHDVDVNLNAHVIFENLVSKASGVLAALHLLDKTDINPEDIEY
                     VLECSEEACGDMNQRGGGNFGKAIAEVAGLLKASGSDVRGFCAAPTHAMISAASMVKA
                     GTFKNVLIVSGGSTAKLGMNGKDHLKKELPILEDVIGGFAVLVSENDGTSPILRTDLV
                     GRHTVGTGSSPQAVITSLVTEPLDRGGLKITDIDKYSVEMQNPDVTKPAGAGDVPNAN
                     YKMIAALGVKRGEIERAALNDFIVDHGMEGWAPTQGHIPSGVPYVGFAKEDIEAGSIN
                     KAMIIGKGSLFLGRMTNQFDGVSIVMEKNPGVVEESKGVSEQEVRGLVAEAMRDFASH
                     LLQG"
     gene            1987..3168
                     /gene="grdD"
                     /locus_tag="VFD79_07900"
     CDS             1987..3168
                     /gene="grdD"
                     /locus_tag="VFD79_07900"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit alpha"
                     /protein_id="HZH94013.1"
                     /translation="MSENNVKAMIGKVFNDIADAMETGQFGQRVRVGITTLGSELGVD
                     NMVKGAELAQKRDSSVEVVLIGPEVDSNLTQVVVDTEEEGYKKMEEMLDSGELGGCVT
                     LHYSFPIGVSTVGRVVTPGRGKEMFVATTTGTSSSHRVEAMVKNGIYGIISAKAMGVE
                     NPTLGILNVDGARQVERAFKELISNGYDLNFTESSRSDGGAVMRGNDLLAGTPDVMVT
                     DTLTGNILMKMFSSFTTGGSYESQGFGYGPGIGEGYDRLVLILSRASGSPVVANAISY
                     AADLVKGGVHAVAKQEFEAVNKAGFKEVLASLTKETKKETSDEKVEAPEKEPVTGTID
                     GIDIMELDDAVEVLWKNKIYAESGMGCTGPIVMVNETKLAEALVVLAKAGYDVGEADP
                     C"
     gene            3314..4693
                     /gene="rpoN"
                     /locus_tag="VFD79_07905"
     CDS             3314..4693
                     /gene="rpoN"
                     /locus_tag="VFD79_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HZH94014.1"
                     /translation="MGHLKLNYDLTLEQSQKLIMTPELRQAIQLLQYTSLELNSYLKK
                     EMEENPLLEMEGPSDVEQSVETLKDEKDIDWKEFLESYDDISYKSEVDRNRKEYSFEN
                     FVSYTPSLKDYLIEQLNMISLGAAEYRIADYIIQNINSNGYLDTSLTEISSQLRVSEE
                     EVEAVLTVIQSFEPYGVGARSLKECLLIQVRDENDFLVERVINDHLEDIATNKIAHIA
                     KELDISIQKAQEYVDIIKCLEPKPGRTFRGDGEDTRYITPDASIEEIDGEYVIFINDI
                     TGPRLNINQYYKSLLKDGGDVETTEFLQDKLNSAMWLIKSIEQRRQTLYKVVQSILKY
                     QKEFLVHGEKRLVPLTLKDVAEDIEMHESTISRATSGKYVQTPKGVFELKYFFTTGLS
                     GKSGEVSATSVKSIIKGIIDREDQKKPLSDQVISNLLEERGMEVSRRTVAKYRDELKI
                     PSSSRRRRF"
     gene            4868..5839
                     /locus_tag="VFD79_07910"
     CDS             4868..5839
                     /locus_tag="VFD79_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HZH94015.1"
                     /translation="MERRYSILRAVDFRQPVGRRALSLELGLKERTVRSEVEKLRELK
                     LLNADNMGMYVTDEGKDLLRQLSEIYQSIRGIPDLRNRLQEKLGIGSIIIVPGDSQSN
                     DLVLQEMGRSASRKLVNILKEDDIVGVTGGSTMAAVSDQITCHRKFPNTTVVPARGGL
                     GKELMTQANSVAEKVGKSLGAMYRLLYIPDSLEREALEVIQSNDEIRQSLQLIERMRI
                     LVFGIGRADILANRRNLSEARIDHLMENGAVAEAFGHYFDIYGNDIWEYKTVGLSLET
                     YREIPQVIGVAGGADKAEAIIAIASIRKDITLIIDEAVGNKIIKILN"
     gene            5856..6863
                     /gene="gap"
                     /locus_tag="VFD79_07915"
     CDS             5856..6863
                     /gene="gap"
                     /locus_tag="VFD79_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004819210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HZH94016.1"
                     /translation="MALKVGLSGFGRIGRDVFRIWAENKVEEFDIVAINASGNIEDLA
                     HLFKYDSVYGKFNGTIETYEEGFVVNGKKIPVIAFRDPSELPWKEMGVDIAIDSTGAF
                     RDREGLQKHLDAGAKKVIVTAPAKGEDLTIVLGVNEDKYDPENHNIISNASCTTNCLA
                     PVAKVILDKLGVKKGLMTTVHAYTNDQMIHDKRHKDMRRARAAAENIIPTTTGAAKAV
                     ALVLPELKGKMNGFSVRVPVPTVSLVDTVFEVDRPTTVEEVNKLLKEASEGELKGIMG
                     FSEEPLVSRDFVGDERSSIIDADSTMVIDNMVKVVSWYDNEWGYSSRVVDLARLVAKK
                     M"
     gene            6934..8121
                     /locus_tag="VFD79_07920"
     CDS             6934..8121
                     /locus_tag="VFD79_07920"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015777697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HZH94017.1"
                     /translation="MNKKTLKDMDVKGKKVLVRVDYNVPMDSEGEITDDIRIRSSLPT
                     VEYLLESGAAVILMSHLGRPKGEPKKEFSMLPVANKLSQLLKKEVVFADDDSVVSDRV
                     KEMASGLNPGGVMLLQNTRFRKEEEKNEDDFAKELASLADLYVNDAFGTSHRAHASNV
                     GVSKYLPSALGYLVEKEVSIMGKALDNPERPFVAILGGAKVSDKIGVIENLLSKVDAI
                     IIGGGMAFTFLRAKGYEVGTSLLEEDKVQLAKDLMVKAKDNGVAMVLPVDVVVAKEFK
                     NDTSFKTVPADGIPADMMGLDIGSESIELFENVIREAKTVVWNGPMGVFEMDNFNKGT
                     YAVANALVKSGAITIVGGGDSASAVEKAGLAEKITHVSTGGGASLELLEGKILPGIDA
                     ISE"
     gene            8134..8880
                     /gene="tpiA"
                     /locus_tag="VFD79_07925"
     CDS             8134..8880
                     /gene="tpiA"
                     /locus_tag="VFD79_07925"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HZH94018.1"
                     /translation="MRKPIIAGNWKMNKTISEAEKLIGEIRMETLDQSVEAVLCVPYL
                     AIGKAKELTDGTGIKIGAQNMHWEESGAFTGEVSPIMLSELGVDYVIVGHSERRQYFN
                     ETDDTVNKKVHSAIAHGIIPIVCVGETLEQRKDNIHKDLVRNQVIGAFKGVDKSAAKK
                     TVVAYEPIWAIGTGETASSDQAEEMCALIRNTLSEIYGDETAEEIRIQYGGSVKPGNV
                     SELMSMDNIDGALVGGASLKSSDFVKLINY"
BASE COUNT         2889 a         1575 c         2309 g         2142 t
ORIGIN      
        1 tggtaatgga acagctgttg ccagttccaa gaaattcgcc aatgagttca gcaaggaatt
       61 aatagctgac ggagttgacg cagttattct aacctccacc tgaggcacct gtacacgttg
      121 cggtgcaacg atggtaaagg agatagaaag agctggaatt ccagtggttc acatttgtac
      181 agtagtgcca atatcactta cagtaggagc aaataggata gttcctgcaa ttgcaattcc
      241 tcatccactt ggaaatcctt ccatggacaa ggtagaggaa aaagccctta gaaggaaaat
      301 agttgagaaa tcactggtag ctcttgaaac agaggttact gagcagacag tatttgataa
      361 ataataagcg actacaaatg ggtgatagta agaactacac ccatttgtaa aatctatatt
      421 taatggaggt gactcgtttg agttattcag tagtaaaggg aacaagctat gtacttgtgc
      481 atgccccaga catggtgctt cacaacggta caacacagac aactgagaaa catctaaatc
      541 caaattcaga gtatctaaag aatctaccaa acagcctaag gacctttgat gaggcggtaa
      601 attatcttcc aaaccaggtt tatattggaa acaataagcc tgcagaccta agaaaggatc
      661 cacagccttg ggttggcaag aatgttgatg gagcatccag aaatggaaag cttggagaaa
      721 taatgccaca ggatgagttc attggcctta tcaagattgt tgacgcattt gaacttgtaa
      781 agcttacaaa agagttcaca gcagacgtca agaaaaaact taatgaacat ccactagtaa
      841 aagatgagtt ggtggctaag cttaaggaag gcgaggatat tgccgacatt caaaagctaa
      901 tcaacgacca gggagcagag cccctttata tgaacaatga ggtaattggt tgcatcaaga
      961 gagcacacga tgttgacgtg aacctaaacg cacacgttat ttttgaaaat cttgtttcaa
     1021 aggcctcagg agttcttgca gcacttcatc tcctggataa gacagatatc aatccagagg
     1081 atattgaata cgtcctagag tgctccgagg aagcttgtgg agacatgaac cagaggggtg
     1141 gaggaaactt tggcaaggcc attgccgagg ttgcgggact attgaaggca tcagggtcgg
     1201 atgttagagg cttctgtgca gcaccaaccc acgccatgat ttctgctgct tctatggtaa
     1261 aggctggaac cttcaagaat gttcttatag tttcaggtgg ttcaactgct aaacttggaa
     1321 tgaacggaaa agaccacctt aagaaggaat tgccaatcct tgaagatgta atcggaggat
     1381 tcgcggtgct ggtaagtgaa aacgatggaa caagcccaat tcttaggaca gaccttgtgg
     1441 gaagacacac tgtgggtaca ggttcttcac cacaggctgt aataacttca cttgtaactg
     1501 agccccttga tagaggtgga ttgaagataa ctgatattga caagtattca gttgaaatgc
     1561 agaatcctga tgttacaaag ccggcaggag caggagacgt accaaatgca aactacaaaa
     1621 tgattgctgc tctcggtgta aaaagaggag aaatagaaag agctgcactt aatgacttca
     1681 ttgtggacca cggaatggaa ggatgggcac caactcaggg acacattcct tcaggagttc
     1741 cctatgtggg cttcgctaaa gaagatattg aggcaggaag tattaacaag gccatgataa
     1801 ttggtaaggg aagtcttttc cttggcagaa tgacaaacca gttcgatgga gtttcaatag
     1861 ttatggagaa aaatcctgga gtagtggagg aaagcaaagg cgtatctgag caagaggtca
     1921 gaggtcttgt agctgaggct atgagagatt ttgcttcaca tctccttcaa ggttagaggt
     1981 gatatgatgt ctgaaaacaa tgtaaaagcc atgattggca aggtgttcaa cgatattgcc
     2041 gacgccatgg agactggcca gtttggacag agggttagag ttggaattac gacccttgga
     2101 agtgaactgg gtgtagacaa catggtaaag ggtgcagagc ttgctcaaaa gagagacagc
     2161 tcagttgagg tggtcctaat tggacctgag gttgattcca accttaccca ggtagtcgtt
     2221 gatacggaag aggaaggcta caagaaaatg gaggaaatgc ttgacagtgg cgagcttgga
     2281 ggttgtgtaa cccttcatta cagcttcccc ataggtgttt ccacagtagg tagggtagtg
     2341 acccctggaa gaggcaagga aatgtttgtt gccaccacaa ctggaacttc atcatcccac
     2401 agggttgagg ctatggttaa aaacgggata tacggtataa tttcagccaa ggccatggga
     2461 gttgagaatc caacacttgg tattcttaat gtggatggtg caaggcaggt tgaaagggca
     2521 ttcaaggagc ttatttcgaa tggctatgac cttaacttca ccgaatcatc aagatctgat
     2581 ggtggagcag taatgagagg aaacgaccta ttagcaggaa cacctgatgt aatggttaca
     2641 gataccctta caggaaacat tcttatgaag atgttctcat cattcactac aggtggaagc
     2701 tatgagtcac aaggctttgg ctatggacct ggaattggtg aaggctatga caggctggtg
     2761 cttatcctgt ctagggcatc aggctctcct gtggttgcca atgcaataag ctatgcagct
     2821 gaccttgtaa agggtggagt gcatgctgta gcaaaacagg aatttgaagc agtgaacaag
     2881 gcaggattca aggaagtcct ggcttccctg acaaaggaaa ctaaaaagga aaccagcgat
     2941 gaaaaggttg aggcacctga aaaagagcct gtcaccggta caatcgatgg aatcgatata
     3001 atggagcttg acgatgctgt tgaggttctt tggaagaaca agatatacgc tgaatcaggc
     3061 atgggctgca cgggaccgat tgttatggtc aacgagacaa aattggcaga ggctttggtt
     3121 gtattggcca aggcaggcta tgatgttgga gaagcagatc cctgctagaa acaaaacccc
     3181 ggaaatttat ttccggggtt tttatgtaac acaaaaagaa ccggtccctt tgtgtttttg
     3241 tagttgttac aaaagtgggc tgttaattgt taatgaccga caattctgat ataattgata
     3301 tagatgaaag gaggtgggac atttgaagtt gaactatgac ctgacacttg aacagagcca
     3361 gaagctcatt atgacacctg aacttagaca ggccattcaa ctactccaat atacaagtct
     3421 cgagctaaat agctacctaa aaaaggaaat ggaggagaat cctcttctag agatggaagg
     3481 cccttcagat gtagaacaaa gtgtggagac actcaaggat gaaaaggaca tagactggaa
     3541 ggagttcctc gaaagctatg atgatataag ctacaagtca gaggtggata gaaaccgcaa
     3601 ggaatattcc tttgaaaatt tcgtgtccta tacaccttct ttgaaggatt atttaattga
     3661 acagctcaat atgataagtc ttggtgcagc ggagtatagg attgcagact atattatcca
     3721 gaacataaac tccaatggct atctggatac aagcttgact gagatttcat cacaactaag
     3781 ggtatcagaa gaggaagttg aggctgtgtt gactgtgata caatcatttg aaccatatgg
     3841 tgttggggca agaagcctta aggagtgtct gcttattcag gtgagggatg aaaatgattt
     3901 cctcgtagaa agagtgataa atgatcacct tgaagacatt gcaacgaaca agattgctca
     3961 cattgccaag gaactggaca tatccataca gaaggcccag gagtatgtcg atataataaa
     4021 atgtctggag ccaaagccgg gtaggacctt taggggagat ggagaggata ccaggtatat
     4081 cacaccagat gcctccatag aggaaatcga cggggagtat gtaattttca taaacgatat
     4141 cactggaccc agattgaaca ttaatcagta ttacaagagc ctgttaaagg atgggggaga
     4201 tgttgaaaca actgagtttc tacaagacaa gctaaactca gccatgtggc tgataaaaag
     4261 cattgagcag agaaggcaga ccctttacaa ggtggtccaa tccatcctga agtatcagaa
     4321 ggagtttctc gtacacggag agaaaagact tgtcccccta acattaaagg atgtggcaga
     4381 agacattgaa atgcacgaat caacaatttc aagggcgaca agtggcaagt atgttcagac
     4441 tccaaagggg gtttttgagc taaagtactt tttcacaaca ggattgtcag ggaaatctgg
     4501 agaagtttca gcaaccagtg taaaatccat tataaagggt ataatagaca gggaagacca
     4561 aaagaaaccg ctgagcgacc aggttatatc caacctgctt gaggaaaggg gtatggaggt
     4621 ttccaggaga acagtggcaa agtacaggga tgaattaaag attccatcat catcccggag
     4681 aagaaggttc taaatatcag agatggcttg aaaaaaagct atctctggta tataatcaag
     4741 gcgtaggata tttttttatc catggtggga caaaataatt gtactgggat aaaaaacgcc
     4801 caatggaggt ggtaacatag atctactaaa attaattgga agactctcac ctgaatgcat
     4861 tcagactatc gaaaggagat actccattct aagggctgtt gattttaggc agccggttgg
     4921 aagaagggct ctttccctgg agcttggatt aaaggaaagg acagtcaggt cagaggtcga
     4981 gaaactaagg gagcttaagt tattaaacgc cgacaatatg ggtatgtatg ttacagatga
     5041 aggcaaagac cttcttaggc agctaagtga aatttaccag agtataaggg gtatccccga
     5101 ccttcgcaac aggcttcagg aaaaactggg gatagggagc ataatcattg ttccgggaga
     5161 ttcccaatca aacgaccttg tgctccagga aatgggaaga agcgcctcaa ggaaactagt
     5221 caacatcctt aaggaggatg acatagtcgg tgtaaccgga ggcagcacaa tggctgctgt
     5281 atccgaccag atcacatgtc acaggaaatt ccccaatact acagtagtac cagctagggg
     5341 aggacttgga aaagaactga tgacacaggc taattcagtg gcagaaaagg ttgggaaaag
     5401 tcttggggcc atgtatagac tcctttatat tcccgacagc cttgaaaggg aggcactgga
     5461 ggtaattcag tcaaatgatg aaattaggca gtcactccag cttattgaaa ggatgagaat
     5521 tcttgttttc ggaataggta gagctgatat acttgcaaac aggaggaacc tgtcagaggc
     5581 caggatagac catcttatgg agaatggagc tgtagcagaa gccttcggcc actattttga
     5641 catctatggc aacgatatct gggaatacaa gacggtggga ttgtcacttg agacatacag
     5701 ggagatccca caggtcatag gggttgcagg tggagctgac aaggcagaag ccataatagc
     5761 aatagcatca atcaggaagg acattaccct gattatcgat gaagccgtag gaaataaaat
     5821 aattaaaata ttaaactaaa caaggaggaa ttttaatggc actaaaagta ggtttaagcg
     5881 gatttggaag aataggtagg gatgtattca ggatatgggc agagaacaag gttgaggagt
     5941 ttgacattgt agccatcaac gcatcgggta acattgagga cctggcacat cttttcaagt
     6001 atgacagtgt ttatggcaaa ttcaacggaa ccatagaaac atacgaagaa ggattcgtcg
     6061 taaacggtaa gaagatacct gttattgcct ttagagaccc atctgagctt ccctggaagg
     6121 aaatgggagt ggatattgca atagactcca caggagcctt cagagatagg gagggacttc
     6181 agaagcatct tgatgctggt gcaaaaaagg ttatcgttac agcacctgcc aagggagagg
     6241 acttaacaat agttcttgga gtaaatgaag acaaatacga cccagaaaac cacaacataa
     6301 tatccaatgc atcctgtaca actaactgtc ttgcacctgt tgccaaggtt atattggaca
     6361 agcttggagt gaagaaaggt ttaatgacta ctgtacacgc atacaccaat gaccagatga
     6421 tccacgacaa gagacacaag gatatgagaa gggcgagagc agcggcagag aatatcatac
     6481 ctaccacaac cggtgctgcc aaggcggttg cacttgtact tcctgaattg aagggtaaaa
     6541 tgaatggatt ctccgtaagg gtaccggttc caactgtatc ccttgttgac actgtatttg
     6601 aggtagacag acctacaaca gtggaagagg ttaataaatt attgaaggaa gcaagtgaag
     6661 gcgaattaaa gggcataatg ggcttctcgg aagagccatt ggtatcgagg gattttgttg
     6721 gtgatgaaag gtcatcaatt attgatgccg actcaacaat ggtaatagac aatatggtta
     6781 aggtagtgtc atggtatgac aacgagtggg gttattccag cagagttgta gacctggcaa
     6841 ggcttgtggc taagaaaatg tagtaattat gtcgggtcat atgacccgac atatcaatta
     6901 aacaaacaat aagaaaagaa ggtggtttgc ttgttgaata aaaagacttt aaaggatatg
     6961 gacgtaaagg gaaagaaggt tcttgtaagg gttgactaca atgttcccat ggattctgaa
     7021 ggtgaaataa cagatgatat aagaattaga agctcacttc caacagtgga gtatctgctt
     7081 gagagtggtg ctgccgtcat actgatgtcc catcttggaa gacctaaagg agaacccaaa
     7141 aaggagtttt caatgctgcc ggtagcaaac aagctttcac agctgcttaa gaaggaggtt
     7201 gtgtttgcag atgatgacag tgtggtttca gacagggtaa aagagatggc aagtgggtta
     7261 aaccctggtg gtgtaatgct tctccagaat accagattca gaaaagagga ggagaaaaac
     7321 gaggatgatt tcgcaaagga gcttgcatcc cttgcagacc tttatgtgaa tgatgccttt
     7381 ggaacctccc acagagcaca tgcatcaaat gtaggagttt caaaatacct gccttctgcc
     7441 ttgggttacc ttgtggagaa ggaagtttca atcatgggca aggcattgga taatccagaa
     7501 aggccatttg ttgccatact tggtggagct aaggtttctg acaagattgg cgtaattgaa
     7561 aaccttctgt ccaaggtcga tgcaattatc attggcggag gaatggcatt caccttcctc
     7621 agggcaaagg gctatgaagt tgggacctcg ctccttgagg aagacaaggt tcagttggcc
     7681 aaagacctta tggtgaaggc aaaggataac ggagttgcaa tggtacttcc tgtggatgtt
     7741 gtagttgcaa aggagtttaa gaatgacacc agcttcaaaa ctgtacctgc agatggcata
     7801 ccagctgata tgatgggtct tgacatagga agcgagtcaa ttgaactgtt tgaaaacgtc
     7861 attagggaag ccaagacggt tgtgtggaac ggtcccatgg gagtttttga gatggataat
     7921 ttcaacaagg gaacatatgc tgttgcgaat gccctggtca aatcaggggc tataacaata
     7981 gttggaggcg gtgactcagc ttcagcagtt gaaaaggctg ggctggcaga aaagatcacc
     8041 catgtttcca caggtggggg agcatcattg gaattacttg aaggcaagat actacctggt
     8101 atagatgcaa ttagcgaata aggaggcagt cttttgagaa agccaataat agctggaaac
     8161 tggaaaatga acaagacaat atccgaggct gaaaagctta tcggagaaat aaggatggaa
     8221 actctcgacc aatcagtgga agcggtactg tgtgttcctt accttgcaat cgggaaagcc
     8281 aaggaattga ctgatggtac tggcatcaag attggagcac aaaacatgca ttgggaggag
     8341 agcggagcct ttacaggtga agtttcacca atcatgctaa gtgagctcgg tgttgactat
     8401 gtgatagtcg gacattctga aagaaggcaa tatttcaatg aaactgacga tacggtaaac
     8461 aagaaggttc attctgcaat agcccatgga atcatcccaa ttgtttgtgt aggagaaacc
     8521 cttgagcaac gcaaggataa tatacacaag gacctggtca gaaatcaggt gattggagca
     8581 ttcaagggtg ttgacaagag tgctgccaag aaaactgtag tcgcctatga gcccatatgg
     8641 gcaattggaa caggggaaac agcatcaagt gaccaggctg aggaaatgtg tgcactgatt
     8701 aggaatacac tgagtgaaat ctatggtgat gaaacggcag aagaaattag gatacaatat
     8761 ggtggaagtg ttaagcctgg gaatgtatct gagcttatgt ccatggataa tatagatggg
     8821 gcacttgtag gcggagcaag cctaaagtca tcggattttg taaagttaat aaactattga
     8881 tcggaggaaa caaatgtcaa aaacaccagt aatgc
//
LOCUS       DASRQF010000295         2138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_512717, whole genome shotgun sequence.
ACCESSION   DASRQF010000295 DASRQF010000000
VERSION     DASRQF010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512717"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VFD79_07930"
     CDS             complement(<1..650)
                     /locus_tag="VFD79_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZH94019.1"
                     /translation="MEEVIARIKKIEKATHNVLRFTLDKPDEIDFIPGQAAEIAVDKD
                     GWRDEAHPFTFTSLPSEEHLEFTIKIYPDYEGVTNELSKLDVGDPFIIKSVFGAISYE
                     GKGTFIAGGAGVTFFISILRNLNAEDKLSGNRLIFANSREKDIINREELESMLGDDFL
                     NILSKEDVESCAHGRIDENYLKKNITSKDDWFYVCGPEAMMDAVIDALENLGVKKD"
     gene            complement(917..1402)
                     /locus_tag="VFD79_07935"
     CDS             complement(917..1402)
                     /locus_tag="VFD79_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH94020.1"
                     /translation="MERSKRIVFVSHCILNQNTVVEPLARSRGAYGDIMRLLVDKGIG
                     IHQMPCPEFRHLGLERRPMIKAEYDTKEYRDLCRGLALDTFGIIEEYKKNSYEILGMI
                     GILESPTCSISKKRGIFMEELMKILDEKNYIINYYDVPADYYDGEKGKAFIQSLEENL
                     K"
     gene            complement(1381..1956)
                     /locus_tag="VFD79_07940"
     CDS             complement(1381..1956)
                     /locus_tag="VFD79_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002835749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HZH94021.1"
                     /translation="MNRNNSSAMIFAGLGIAINIVLGSVANFISIPLLYLDTIGTIFT
                     GAVFGPFYGALTGGLTNVIQGIISNPKNIPFALVNIAVGVIVGLIARKWKFDVKTAVI
                     TGLILAVVAPLIGTPIATYVYGGITGDFNDVVFTWLVQSGQKIFTAAFIPRITSNFVD
                     KIASAVIVALLIKKLPRDLFKGANRWKEAKE"
BASE COUNT          586 a          486 c          421 g          645 t
ORIGIN      
        1 ctatccttct tgactcccaa attctcaagg gcatcaatca ctgcatccat catagcctca
       61 ggtccgcata catagaacca atcatccttc gaggttatgt ttttcttcag gtaattctca
      121 tcaatacgtc catgggcaca ggactcaacg tcctccttgg acaggatatt gaggaaatca
      181 tctcccagca tggattctaa ctcctcccgg ttgatgatgt ccttctccct gctgttggca
      241 aatataagtc ggtttcctga aagcttatcc tcagcattga gatttcgaag gattgaaata
      301 aaaaatgtga cacctgctcc ccctgctatg aaggttcctt ttccctcata acttattgct
      361 ccaaaaacac tcttgattat aaaggggtct cccacatcaa gctttgacag ctcattggtc
      421 acaccttcat agtcaggata gatcttgatg gtaaactcca aatgttcctc tgatggaaga
      481 gatgtgaaag tgaagggatg tgcctcatcc cgccatccat ccttgtcaac tgcaatttca
      541 gcagcctgtc cagggataaa gtcaatctca tctggcttat caagggtgaa acgcagtaca
      601 ttatgtgttg ccttttctat ttttttgatt cttgcaatta cttcttccat gtctaatacc
      661 tccactataa ttcaaaagaa tgaacttcca acagcttacc atattgtttc ccaagcgtca
      721 caatatcttc aaatattgca acccttatat atgtttctac cccaatgtat agtatagaat
      781 caggatcaag ggctgggagg gcggaagcaa agtaaatagt ggattctgaa aaaatgatac
      841 cttttgggag taattaaaaa ccacctgcaa tatgtccatc attgcttggt ccatttgcag
      901 gtggttcatg ttcctattat tttagatttt cctcaaggga ctggataaaa gccttgccct
      961 tctccccatc gtaatagtcc gcaggtacat cataatagtt gataatatag ttcttctcat
     1021 caaggatttt cataagctcc tccatgaaga tgcccctttt cttggagatg ctgcaggtgg
     1081 ggctttccag gatgccaatc atacccagga tttcatagga gttctttttg tactcctcaa
     1141 ttattccaaa ggtgtcaagg gcaaggcctc tgcagaggtc cctgtactcc ttggtatcat
     1201 attcagcctt gatcatgggt cttctttcca atcccaagtg cctgaactcg ggacagggca
     1261 tctggtgtat tccaattccc ttgtccacaa gaagcctcat aatgtcccca taggctcccc
     1321 tgctccgtgc cagtggctcg acaacagtgt tttggttcaa tatgcagtgt gacacaaaga
     1381 ctattctttt gcttctttcc atctgttggc tcctttaaat aggtctcgtg gaagcttctt
     1441 gataagcagg gccactataa ctgcactggc aatcttgtcc acgaagttgc ttgtaatcct
     1501 tgggatgaag gcagcggtga atatcttctg gcctgactga accagccagg tgaatacaac
     1561 atcattgaaa tcacctgtaa tccctccata tacgtatgta gcgatgggtg tgccaatcag
     1621 tggagccacc acagccagaa taagtcctgt gataactgca gtcttcacat caaacttcca
     1681 tttccttgcg atgagaccta caatcacccc aactgcaatg ttcacaaggg cgaatggaat
     1741 gttctttgga tttgagatta taccctggat gacatttgtc agtccgcctg taagtgcacc
     1801 gtagaagggt ccaaagacgg cacctgtaaa tattgttccg attgtatcaa ggtacaaaag
     1861 gggaatgctg ataaagtttg ccactgaacc aaggactatg tttatggcta ttcccagtcc
     1921 ggcaaagatc attgctgatg agttgtttct gttcatatgt aatcctccta aaattagtct
     1981 agaatcatta taagtgaagg aatgtggaaa gttaaattga attatttaat atctaaaaca
     2041 gggctattag attttcttat atgacgcgta ctaaatatca gaaaaatttg aataatacct
     2101 ggaaataggc tggaattacg gtataatatg ggtaacgg
//
LOCUS       DASRQF010000296         4740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1424830, whole genome shotgun sequence.
ACCESSION   DASRQF010000296 DASRQF010000000
VERSION     DASRQF010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424830"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VFD79_07945"
     CDS             <1..349
                     /locus_tag="VFD79_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZH94022.1"
                     /translation="VKLAGDFFREDEDEWILKNRDYYRVYKVLENGDLVGFGVVETGR
                     VVKSIASIGMFVMEDKRRLGYAKNILRQLKELVEEEGYIPRSGCWYYNHSSLKSMISA
                     GGYPKSRLLKFYF"
     gene            468..1040
                     /locus_tag="VFD79_07950"
     CDS             468..1040
                     /locus_tag="VFD79_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HZH94023.1"
                     /translation="MVTLEQVEKLREYANISYEEAKLALEETDGDILEAIVNLEKRNR
                     INRPEGGGSFNSDKSKEESQERKSNTGGTTYTKSQGSSFGEMVGKFFRSLGEVVGKGN
                     RNTFDVMKGEERVMSIPVTIMAILLLFTFWVTIPLVIVGLFFGYRYRIVGPDLGKENV
                     NKAMDSVADAAEKFRDEVKGDMKDGKDSNN"
     gene            1018..1707
                     /locus_tag="VFD79_07955"
     CDS             1018..1707
                     /locus_tag="VFD79_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZH94024.1"
                     /translation="MEKILIIEDEEKIARFIELELKYEGYEVEKALNGRDGLELATTQ
                     PFDLILLDIMLPELNGIEVLRRIRRVSDVPILLLTARDGVVDKVAGLDGGADDYITKP
                     FAIEELLARIRVGLRKRTTDNTSRASNEISAGRLLLDPVKREVKVDGTIIDLTKKEFD
                     LLNYLMENKNIVISRETILEHIWGYDFSGETNAVDVYIRYLRSKIEEPFGLKTIHTVR
                     GVGYVIKDEKD"
     gene            1694..3151
                     /locus_tag="VFD79_07960"
     CDS             1694..3151
                     /locus_tag="VFD79_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZH94025.1"
                     /translation="MKRTKSAGSGTIKNRIQSSLVLRLNVRMMGRLLAGFISINIVII
                     LISFFVVFWNVENGIDGMVQLKGNLPEEINLEFGNYQIAQEEILSGFELNQTFLEYID
                     RNRTGGARTLGISEADLEKNLVDRLQNIKYLAQIQVDGIDIFIYYPIGNDFMILLGLL
                     LIILGFEVIILSTGFVKGSRMIRKTLRPLNELAEAARSLNQEVTSMSSKSQDKYLKDL
                     AGAISSIDANRLDEQISVDSSQEELKGLAAAINQMLNRINDSYQSQVQFVSDASHELR
                     TPISVIQGYVNLLDRWGKNDEKTLQESIDAIKGETESMKELVEQLLFLARGDNETIQL
                     HKESFDVCEVIHDIAREAEMIDKGHEFQMDLKCKAFISADKQLIKQAIRILVDNSIKY
                     TPEGEKIILRVAENEGDIRITVQDSGIGIPPESITRIFDRFYRSDESRARKTGGSGLG
                     LSIAQWIIERHGAHFEVLSRVDIGTRITIVMPRAD"
     gene            complement(3235..3885)
                     /locus_tag="VFD79_07965"
     CDS             complement(3235..3885)
                     /locus_tag="VFD79_07965"
                     /inference="COORDINATES: protein motif:HMM:NF021485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HZH94026.1"
                     /translation="MKKSIKRSLGTFMALGVILASSSGITAFAESDDIGASGALSESS
                     FELEEMLDYAMEDEFLALEEYEAIIEKFEVDRPFSNIMRAEETHIKLLEPLFKEYNIQ
                     IPSEDWETKVAIPESLEEAYEIGVEAEIKNIEMYERFLEQDLPDDVRLTFERLKTASE
                     HHLAAFERQVERESSTFGNWERGNGRANGNHEKSSGGFGGNNQRDRGLNLVDCPIG"
     gene            3848..4435
                     /locus_tag="VFD79_07970"
     CDS             3848..4435
                     /locus_tag="VFD79_07970"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HZH94027.1"
                     /translation="MKVPSDLFIDFFIFYNAIQKNANAGFAALAGFVFMFFSNKIIKY
                     KTKQEVLAFGITALEGIGIHSFIDGITYAVTFSASIYTGLLAGIGLVIHEFAEGVTTF
                     SVLVSGGISEKKAQIFAFLVAGLTTPIGAFVAFPFVSGLSSDLLGLALGFVVGVLIFI
                     SASHLLPEATAHEKGHSYIALISGIGLSLLIMLTK"
BASE COUNT         1428 a          852 c         1145 g         1315 t
ORIGIN      
        1 tgtaaaactt gctggagatt ttttcagaga ggacgaggat gaatggattc ttaagaatag
       61 ggattattac agggtttaca aggtacttga gaatggagac ctggttggat ttggagtggt
      121 cgaaacggga agggtcgtca aatcaattgc aagcataggc atgtttgtta tggaagacaa
      181 gaggcggctg ggctatgcaa agaatatcct tagacagctt aaggagctgg ttgaagaaga
      241 gggatacatt ccaaggtctg gatgctggta ctataatcac agttccctaa aaagcatgat
      301 aagcgctggg ggatatccaa agagcagact tcttaagttc tacttttagg ggagctcaga
      361 ttaatgctaa aaatttcaat tctaatgaaa ttttaatctt tctcaaattg tgtttaaagg
      421 ttgcactgct acaattaaac catactaaac aataggaggt ttgagatatg gtaactttgg
      481 aacaggtaga aaaattaagg gagtatgcaa acatttcata cgaggaagca aaattggctc
      541 ttgaggagac agacggtgat attcttgaag caatagtaaa tcttgagaag aggaacagga
      601 tcaacaggcc tgagggtgga ggaagcttca actcagacaa gtctaaggag gagtctcaag
      661 aaagaaaaag caacaccggt ggtaccacct acaccaagtc acaagggtca tcatttggtg
      721 aaatggtagg gaaattcttc agaagtctag gagaggttgt tggcaaggga aacagaaata
      781 cctttgatgt gatgaagggt gaggaaaggg tcatgtctat accggtaaca ataatggcga
      841 tacttcttct ctttacattc tgggtgacaa taccgcttgt aatcgttgga ctattctttg
      901 ggtataggta tagaatagta ggacctgacc ttggaaagga aaatgtcaac aaggcaatgg
      961 actcagtggc agatgcagct gaaaaattta gggatgaagt taagggtgat atgaaggatg
     1021 gaaaagattc taataattga agatgaagaa aaaatagctc gtttcataga gctggagctt
     1081 aaatacgagg gctatgaagt tgagaaggca ctaaatggaa gggatgggtt ggagcttgcc
     1141 acaacccagc cctttgacct tattcttctt gatatcatgc tgccggaatt aaatggaatt
     1201 gaagtcctaa gaagaatcag aagggtttct gatgtcccca ttttgctcct tactgcaagg
     1261 gatggggtag ttgacaaggt ggcaggtctt gatggtggag cggatgacta tataacaaag
     1321 ccctttgcaa tcgaggaact ccttgccagg atcagagttg gcttgaggaa aaggacaacc
     1381 gacaatacat ccagagcatc caatgaaatc tcagcaggca gattgttatt ggatccggtt
     1441 aaacgagagg tcaaggtgga tggtacaata atcgacctta caaagaagga gtttgacctg
     1501 ctcaattatc ttatggagaa caaaaacatc gtaatcagtc gggagacaat ccttgagcac
     1561 atatggggct atgatttttc cggggagacc aacgcagtgg atgtgtacat aagatatctc
     1621 cgttcaaaaa tcgaggaacc ctttggcttg aagacaattc atacagtacg cggagtgggg
     1681 tatgtgataa aggatgaaaa ggactaaaag cgctggatcg ggtacaatta aaaacagaat
     1741 ccaatcatct ctggttttaa ggcttaatgt aaggatgatg ggaagactgc ttgcaggatt
     1801 tatctcaata aacattgtta taatcctgat aagttttttt gtggtttttt ggaatgttga
     1861 gaacggaata gatggaatgg ttcagctgaa gggcaacttg cctgaggaaa tcaacctgga
     1921 gtttggaaat tatcagattg cccaagagga gatattatcc ggctttgaac ttaatcagac
     1981 atttctagag tacattgata ggaaccgcac cggaggagct aggactcttg gaatctctga
     2041 ggctgacctg gagaagaacc ttgtggacag gcttcaaaac ataaagtatc tggcccagat
     2101 tcaggttgat gggattgata tcttcattta ctatccaatc ggcaatgatt ttatgatcct
     2161 actgggttta ctccttataa tacttggatt tgaagtgatc atactttcaa cgggatttgt
     2221 aaaggggtcg cggatgataa gaaaaacatt gagacctttg aatgaactgg ctgaagcagc
     2281 aagaagcctg aaccaagagg taacctcaat gagctcgaag tcacaggata aatacctgaa
     2341 ggatctggcg ggagcaataa gcagtattga tgccaacagg cttgatgaac agatttccgt
     2401 ggatagctcc caggaggaac tcaagggcct tgcagctgcc ataaaccaga tgctgaacag
     2461 gataaatgat tcctaccaat cacaggttca gtttgtttca gatgcatctc acgaactcag
     2521 gactcccata tcggtgatac agggatatgt gaatctactg gacaggtggg ggaagaatga
     2581 tgaaaagaca ctacaggagt caatagatgc cataaagggc gagacagaga gcatgaagga
     2641 attggttgag cagctattgt tcctagcccg gggagataat gaaacaatcc agcttcacaa
     2701 ggagagcttt gatgtttgtg aagtgattca tgacatcgcc agggaagctg aaatgataga
     2761 taagggtcat gagttccaga tggacttgaa gtgtaaggca tttataagtg ctgacaagca
     2821 gctaataaaa caggcaatca ggatactggt ggacaacagc atcaagtata cccctgaagg
     2881 cgaaaagata atccttaggg ttgctgaaaa tgagggcgac ataaggatta cagttcagga
     2941 tagtggaatt ggaataccgc ctgagagcat cactagaatt tttgacagat tctacaggtc
     3001 agacgaatca agggccagaa aaacaggggg ttcgggtttg ggattgtcaa ttgcccaatg
     3061 gatcatagaa aggcatggtg ctcattttga ggttctaagc agggtggaca tcggaaccag
     3121 aattaccatt gtcatgccgc gggctgattg agtcaccggg gacggttctt tttgactcac
     3181 ttccagcaac tgtaggggtg caccgaccaa tcggtgcacc cctttctaga ttccttatcc
     3241 tataggacag tctacaagat taagacccct atctctctga ttattccctc caaatccacc
     3301 tgatgatttt tcatgatttc catttgctct tccatttcct ctttcccagt tgccaaatgt
     3361 ggaagattct ctctcaacct gtctttcaaa ggctgcaaga tggtgctctg atgctgtttt
     3421 cagcctttca aatgttaatc tgacatcatc aggcagatcc tgctcaagaa acctttcata
     3481 catttctata ttcttgatct cggcctccac accaatctca tatgcctctt ccagactttc
     3541 tggtatggcc acctttgtct cccagtcctc tgaagggatt tggatgttgt attctttgaa
     3601 cagaggctct aaaagtttaa tatgggtttc ttcagccctc atgatatttg aaaaaggcct
     3661 gtccacctca aacttttcaa ttatcgcttc gtattcctcc aaagcaagaa attcatcctc
     3721 cattgcgtaa tccagcattt cctcaagttc gaagctgctt tcagagagag ctccgcttgc
     3781 cccgatatca tcgctttcag cgaaggctgt gattcctgag ctacttgcaa gtataacccc
     3841 aagtgccatg aaagttccaa gtgatctttt tattgatttt ttcatttttt acaacgccat
     3901 ccaaaagaat gccaacgctg gattcgctgc attggctggc tttgttttca tgtttttcag
     3961 caacaagata ataaagtata agaccaagca ggaagtgctt gcctttggaa tcacagcact
     4021 tgagggaata ggaatccact cctttataga tgggataaca tatgcggtca catttagcgc
     4081 aagtatctac actggtcttc ttgcggggat tggacttgtg attcatgagt ttgcagaagg
     4141 ggtcacaaca ttttcagtcc ttgtgagcgg aggcataagt gagaagaagg ctcaaatttt
     4201 tgcttttctg gtggcgggac tgaccacgcc aataggtgca tttgttgcat ttccgtttgt
     4261 aagtggtcta agtagtgatt tacttgggct tgctcttggt tttgtagtgg gggtactgat
     4321 cttcatttct gcatctcacc ttctccctga agcaacggcc catgaaaagg gacattccta
     4381 cattgccctt atatcaggga ttgggctctc gctcttaatt atgcttacaa aatgaagttc
     4441 tacaatcatt gaacagtaga tgatggaaat tttttaaaag cttaattact aaattgagat
     4501 ttttgtgtta taatgatacc gtagtaaatc aatagaaagg caaggtattg tatacgatga
     4561 agtaagatcc actactttaa gaactagatg caaatatcaa gaaccctaat gaggttaatt
     4621 tgatgtgtgc aaatttagat taatgagtgg atagtattgt atttatcgta tgtagagata
     4681 tttgtctggg aattaccttt ttaattaatt gcatttgtac tgcaaaaggg gtagttctgc
//
LOCUS       DASRQF010000297         4791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1412846, whole genome shotgun sequence.
ACCESSION   DASRQF010000297 DASRQF010000000
VERSION     DASRQF010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412846"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VFD79_07975"
     CDS             <1..481
                     /locus_tag="VFD79_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZH94028.1"
                     /translation="VKLNSLQDRIKILHTDLKNLPETLGKASVDTVITNPPYMKPGGA
                     IVNREDNFAISRHEIAATLEDIAAVGQYILKPLGKLYMVHRPNRLVDVLCTLRKHGLE
                     PKYMQWVHPKPGKQPNLFMVEAVKGGKSDFKYRDPIVVYDENGKYNEEIYEIYGMSR"
     gene            486..1310
                     /gene="rsmI"
                     /locus_tag="VFD79_07980"
     CDS             486..1310
                     /gene="rsmI"
                     /locus_tag="VFD79_07980"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HZH94029.1"
                     /translation="MEAGTLYICPTPIGNLDDITLRVLKILKEVDLIAAEDTRHTLRL
                     LNHFQIKKPMTSYHEHNIREKGPHLVQELQEGRTIALVSDAGMPGISDPGQDLIRLAI
                     EEGIEVVGLPGPSAALLALVISGLPTEKFAFEGFLPSRSTDRKKALESIRDERRTLIF
                     YESPHRIAGSLQDMVAVLGDRQASLSRELTKHYEETIRGGLEEILSVANERTLKGEMV
                     LVVRGAEVVEAEEVDIKEELRRLLEGGMTKKEAVKELVRVHGLPRNQVYQESLDLD"
     gene            complement(1368..1610)
                     /locus_tag="VFD79_07985"
     CDS             complement(1368..1610)
                     /locus_tag="VFD79_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HZH94030.1"
                     /translation="MKSTGIVRKVDELGRVVIPIELRRNLDIQVKDPLEIFVDEDKVI
                     LKKFSPSCIFCGESKGVEDFKGKNICQGCLEELSAK"
     gene            1721..2695
                     /locus_tag="VFD79_07990"
     CDS             1721..2695
                     /locus_tag="VFD79_07990"
                     /inference="COORDINATES: protein motif:HMM:NF020933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HZH94031.1"
                     /translation="MYPLNGELKTKLQTINTAIQEKYGLILDGDKNRLKDFIGNEIGL
                     VKKIEPLTPQELADLEERGGICGVDGSSNRAGGNFPHFIEVYQALALTTIKREDPLYL
                     AEIFTPVITDEEDQEGEEREEQKNQKLAGLEVQAAIRSIQVHKPYAILMDGGLIRYNI
                     YAGDVWEDLVRLCEETGTILVGVIKDIKTRVIGDRLKKAYPDLKGNLYDRELLFGLLD
                     YKDLLLIDESVSKKEPMGYSSAFLRSSLSPMAIGLDIIESQREHLTQMARLVLSLTPQ
                     NSRGVPLWIDIVDREVKISDQMMKGMLERYLDRGVYERFFVSERDKRN"
     gene            3069..>4791
                     /locus_tag="VFD79_07995"
     CDS             3069..>4791
                     /locus_tag="VFD79_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZH94032.1"
                     /translation="MKVVGITTQQEFYVGSKERNFRINEFLLIQDPFQEDLIGEVVEA
                     RTFNRYIPLDIGGDFVDNAVLESLRALGYDVDEDTIYIAKVRLLNEALYPVQTGSDVR
                     LPKFHEIKDIMVKTDPENGLVLGVIRNTDDVAQDMDEKLKDILYTFEEGGLKRQRELP
                     FIMDYRSMHHYPHIGVFGGSGSGKSFGLRVLLEEIMKKEIPTIVLDPHFEMDFSESPE
                     YLKEGEESFASMFACLQVGAHVGVRFEDLSAGDLKNLLDAASPLSDAMNGVVDVMFKR
                     RDSFTSFLGRLEGLTEAQEEGSLERIERRIAGATSQSERESWEDRKDLYLSYDKTCNY
                     NSVKGVLWRLRRLSNEGIFSKDSREIENKLQDGKLVVIQGSTRILQVYSTYLLNNIYH
                     KRRDYKDSLYKREAGDYFPPFMIVTDEAHNFAPKGYDSPSKSILREISQEGRKYGVFL
                     ILATQRPTLLDETITAQLNTKFIFRTVRASDIDTIREETDITQEEAKRLPYLRTGDVF
                     ISSALMGRTLFARVRAAYSSSPHTLNPFDELRKQSREDEEKYLQLIMEKLPIMESDLL
                     GVIQDLEK"
BASE COUNT         1421 a         1054 c         1231 g         1085 t
ORIGIN      
        1 tgtaaagctg aactcacttc aggacaggat aaagatactc catacagacc taaagaacct
       61 gccggaaacc cttggcaagg caagtgtgga tacagtcata accaatccac catatatgaa
      121 gcccggcgga gccattgtca acagggagga caactttgcc atctcaaggc atgaaatagc
      181 cgccaccctg gaagacatcg cagctgtagg ccaatacata ttaaaacccc tgggtaagct
      241 ttacatggta caccggccca acagactggt ggatgtgctt tgtaccttaa gaaagcacgg
      301 cctggagcca aaatacatgc aatgggtcca ccccaagcct ggcaaacagc caaacctatt
      361 tatggttgag gctgtaaagg gtggaaagag tgattttaag tacagggacc caattgttgt
      421 ttacgatgaa aatggcaagt acaacgaaga gatttatgaa atctacggaa tgagcaggtg
      481 agagaatgga agcaggaaca ctgtatattt gtccaacacc aataggaaac cttgatgaca
      541 ttacccttag ggtactgaaa atacttaagg aggtggacct gattgctgcg gaggatacca
      601 gacataccct caggctcctc aaccacttcc agatcaaaaa gcccatgacc agctaccatg
      661 agcacaacat cagggaaaag ggaccccatc tggtacaaga gcttcaggag ggaaggacaa
      721 tagcccttgt atccgatgcg ggaatgcccg gaatatcaga tcctggacag gacctaataa
      781 ggctggcaat agaagaggga atagaggttg tgggacttcc agggccatcg gcagcacttc
      841 tggcacttgt gatctccggg cttccgacgg aaaaattcgc ctttgaaggt tttctccctt
      901 caaggagtac agataggaaa aaagcccttg agagcataag ggatgaaagg agaaccttga
      961 ttttttatga aagcccccac aggattgcag gcagcctcca ggatatggtg gctgttcttg
     1021 gagacagaca ggcatccctt tccagggagt tgaccaagca ttacgaggag acaatcagag
     1081 gtggattgga agagatcctc tctgttgcaa atgaaagaac ccttaagggg gagatggtcc
     1141 ttgttgtaag gggtgctgaa gtggtcgaag ctgaagaggt ggatataaag gaggagctta
     1201 ggaggcttct tgagggtgga atgaccaaga aggaagccgt caaggaactt gttcgtgtcc
     1261 atggactacc tagaaaccag gtttaccagg aaagtctgga cctggattag ggcataaaaa
     1321 aacataaaaa aacactcgga agggtccgaa tgctttttta tgtggtccta ttttgccgaa
     1381 agctcctcta ggcagccctg gcagatattc ttgcctttga aatcctccac acctttggat
     1441 tcgccacaaa agatacatga tggggaaaac ttcttaagga taaccttgtc ctcatctacg
     1501 aatatctcca gtgggtcttt aacctggatg tctagatttc ttctgagctc tataggtatc
     1561 actactctac ctagctcatc cactttacga acgattccag tggatttcat ctaaccacct
     1621 cccaattagg ttaacataat aacctaacca aaatattacc atactttcca taaaaagtca
     1681 ataattttca agtggcatta caatgaagtg agggaaaata atgtatccat taaacggtga
     1741 acttaaaaca aaactccaga ccatcaatac agccatccag gaaaagtatg gtttgatcct
     1801 ggatggggac aagaataggc ttaaagactt cattggaaat gaaataggcc ttgttaaaaa
     1861 gattgaaccc ctgacaccac aggagcttgc agaccttgag gaaaggggag ggatatgtgg
     1921 ggttgacgga tccagcaatc gggcaggagg aaactttcca cactttatag aggtttatca
     1981 ggcccttgcc ttgaccacca taaagaggga ggaccccctt tacctggcgg aaatattcac
     2041 tcccgtcatc acagatgaag aagaccagga gggtgaagag agggaggagc agaaaaacca
     2101 gaagcttgcg ggccttgagg ttcaggccgc cataaggtcc attcaggtcc acaaacccta
     2161 cgccattctc atggacggtg gactgataag gtacaatatc tacgcagggg atgtgtggga
     2221 ggacctggtc cgcttgtgtg aggaaacagg aaccatactt gtgggtgtga tcaaggacat
     2281 caagacaagg gttattggag accggctgaa aaaggcctac ccggacctta agggaaacct
     2341 ctatgacagg gagcttctct ttggtcttct ggactacaaa gacctgcttc tgatagatga
     2401 gtctgtaagc aagaaggagc ccatgggcta cagttccgca tttctcagaa gctcactttc
     2461 acccatggct atcgggttgg atataattga gagtcagagg gaacacctga cccagatggc
     2521 cagactcgta ctctccctta caccgcagaa cagtcgtgga gtccccctat ggatagacat
     2581 cgtagacagg gaggtcaaga tttccgacca gatgatgaag gggatgcttg agaggtacct
     2641 ggaccgggga gtatatgaga ggttttttgt atcggagagg gataagagaa attgagaaga
     2701 caattctcaa ttctcaattc acaatgcaca atttattggg atgccagcca agatcataag
     2761 actcttcgct tggctcagag tgacaaatac taagtcatct tgagtgaaac taaggatcct
     2821 atagctttgg agtcgtcctc ttgaaaatgt taattgtgag ctgcttatgg aattgatttg
     2881 atttggtcta ttaactaaag aatctttttc attgtgaatt gttatttgct tgtgcacagc
     2941 aatagaaata tccagaattt ttgattctga gatatttcag tgacagggta tgaactgtga
     3001 attagagaag gtttttcatt gtgaattgtg aattgtgaac tgtgaattat taagtggggt
     3061 gttaaaaaat gaaggttgta ggaataacaa cacagcagga attttacgta ggctcaaagg
     3121 agaggaactt ccgcataaac gagttcctcc ttatccagga ccccttccag gaggacctga
     3181 ttggcgaagt ggtggaggca aggaccttca accgctacat tcccctggac atcggagggg
     3241 actttgttga caacgcagtc cttgaaagtc taagggccct tggctatgac gtggacgagg
     3301 acaccatcta catcgcaaag gtaaggctcc ttaacgaggc actttatcct gtccagaccg
     3361 ggtcggatgt caggctgcca aagttccatg aaatcaagga catcatggta aagacagacc
     3421 ctgaaaacgg cctggtactg ggagtcataa gaaacaccga cgacgtggcc caggacatgg
     3481 atgaaaaact caaggacatc ctctacacct ttgaggaggg gggattgaaa agacagcggg
     3541 agcttccatt cataatggac tacaggtcaa tgcaccacta tccccacatt ggggttttcg
     3601 gagggtccgg ctcaggtaag tccttcgggt tgagggtcct ccttgaagag atcatgaaaa
     3661 aggaaattcc tactatagtt ctggaccccc attttgagat ggacttctcc gagagtccag
     3721 aatacctgaa ggagggtgaa gaaagcttcg cctccatgtt tgcatgtctt caggttggag
     3781 cccatgtagg tgtaaggttt gaggaccttt cagcgggaga tttgaagaac ctcctggatg
     3841 cggcaagtcc cctttccgat gccatgaacg gggttgtgga tgtgatgttt aagagaaggg
     3901 actccttcac tagcttttta ggcaggcttg agggcctgac cgaagcccag gaggaagggt
     3961 ccctggagcg aatagaaaga aggatagcag gtgccacatc ccaaagtgag agggaatcct
     4021 gggaggacag gaaggacctc tacctatcct acgacaagac ctgcaactac aattcggtga
     4081 agggcgtcct gtggaggctg agaaggctct ccaacgaggg catattctca aaggacagca
     4141 gggaaatcga gaataagctc caggatggaa agcttgttgt aatccaggga agcaccagga
     4201 tcctccaggt ctacagcacc tatctgttga acaacatcta ccacaagaga agggactaca
     4261 aggattccct atacaagagg gaagcgggag actacttccc acccttcatg atagtcacag
     4321 acgaggctca caactttgca cccaagggct atgattcccc ttcaaagtcc atactccggg
     4381 aaatatccca ggagggaagg aagtacggag tcttccttat ccttgcaacc cagaggccca
     4441 cgcttcttga tgagaccatc acggcacagc tcaacaccaa gttcatcttc aggaccgtac
     4501 gtgcctctga catagatacg atcagggagg aaaccgacat aacccaggag gaggccaaga
     4561 gattgccata cctcaggacc ggtgatgtct tcatatcctc agcccttatg ggaaggaccc
     4621 tctttgcaag ggtcagggct gcctacagct caagccccca caccctgaac ccctttgacg
     4681 agttaaggaa gcagtcaagg gaggatgagg aaaagtacct tcaactgatc atggaaaagc
     4741 tgcccatcat ggaatcggac ctgcttggag tgatccagga cctggaaaag g
//
LOCUS       DASRQF010000298         4819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1542577, whole genome shotgun sequence.
ACCESSION   DASRQF010000298 DASRQF010000000
VERSION     DASRQF010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542577"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="VFD79_08000"
     CDS             <1..1179
                     /locus_tag="VFD79_08000"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HZH94033.1"
                     /translation="TDLLYMCHQLSSHNLHFEEVFQLIRDRYEGICRDLDLETPIGQI
                     LDKVREDISKGASKDYCASRGEYINGRILADYLESDFVDAAQVIRFDKRGVFDQAETE
                     RLVMERLSDVKRAVVPGFYGADGDGNIVTFSRGGSDVTGSILADGVGARLYENWTDVS
                     GFLKADPKIVKDPLPIREVTYKELRELSYMGAPVLHEESIFPIKNKKIPIQIRNTNKP
                     QDQGTLIVNDSWAGVDPGIITGIAGKKDFTVISVEKTRMTGEKGFLRKLVSVFEAKDV
                     AIEHMPSSIDSISVIVSDKEMNGKLSKLMEEIRIYCNPDNITAHSKMALLAVVGRGMI
                     NTKGVSSRIFTALYKDDINIRMISQGSSELNILIGIENDDFEKSVRAIYNAFNEREVS
                     "
     gene            1180..2172
                     /locus_tag="VFD79_08005"
     CDS             1180..2172
                     /locus_tag="VFD79_08005"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HZH94034.1"
                     /translation="MKKFNVAVVGATGLVGRTILQVLQEEDFPIENLYPMASERSEGD
                     LIQFRDKEYKVIKVSDQAFDEDIDIAFFAAGGAISERYAPVAAEKGIRVVDNSSVFRM
                     NPEVPLVVPEVNPQDIKKSKGIIANPNCSTIQCMLPLKALQDSYGIERVVYSTYQSVS
                     GSGTKGLRDLDEGLNEFYPYPIKGNVLPHIDVFLDNGYTKEEMKMIEETRKILHDEDM
                     KVTATAVRVPVRFAHSVSANVQLSRNFDLDVVRKMLGDYPGIILKDDPDNGQYPIPQD
                     AEGKNEVYVGRVRRDFSQDNTLNLWIVADNIRKGAATNAVQIGKLLVDNWEEMK"
     gene            2169..3377
                     /locus_tag="VFD79_08010"
     CDS             2169..3377
                     /locus_tag="VFD79_08010"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HZH94035.1"
                     /translation="MTNIGLLGFGTVGQGVYRILQEKGEDLKVLLGREIEVKSILVRD
                     LYKEREIQVPLSKLTLNPEDIVSDEDIDIIVEVTGDVDLSYRIISKAFERGKHVVTAN
                     KAVVSAYFEELSHLAEENGVFFLYEASVAGGIPVLKPLKDQIRLNEITRIQGILNGTC
                     NYILTRMTHEGLAYGDVLKEAQRLGYAEADPSSDVEGIDTMRKLRIMSTLALGASITE
                     DDIICDGIDKISDLDINLLRNKGMKVKLIAEAREVEGGYQAIVQPKALPVESYFAGVN
                     GAYNGISMDGNYSGTLGFQGPGAGMYPTANAVLTDVIDCILETQRKMSPLRGRKLENR
                     NEDIAGRYYLRISGYDGSMENIRDQAEEVLLDRDGEFAFITGQVKLYHLLQGLAEHED
                     KPFSLIAIEQ"
     gene            3387..>4819
                     /gene="thrC"
                     /locus_tag="VFD79_08015"
     CDS             3387..>4819
                     /gene="thrC"
                     /locus_tag="VFD79_08015"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZH94036.1"
                     /translation="MRYYSTRNKDNVVSSMEAIIKGIAEDGGLFVPEAFPALPKLEDI
                     RNLTYKELAKLILGLYFDDYPEEDLEEAVNGAYGDKFGNREPVRLVDKGGVNFLELFH
                     GPTLAFKDMALTILPYLMRSALLSKGVEEEVVVLAATSGDTGKAALEGFRDVPGVNVV
                     VFYPEGGVSQLQRLQMITQKGENTHVLAIEGNFDDAQKGVKDIFGDLDYNKELKEEGY
                     VLSSANSINIGRLIPQVVYYVYAYLDLLRKGRIEVGQEVNIVVPTGNFGNILAAYYAK
                     MMGLPVDRLICSSNDNKVLTDFFETGTYDRRRDLLLTSSPSMDILVSSNLERFLFHIS
                     GNDHQAVREMMEDLSREGYFKWSDFSREEIYGEFADEDEVSSAIRDLYESEGYVMDPH
                     TAVAYRVYKKYLKETGDSKPAIIASTASPFKFPEKVISSMGEEVPQDLFTGIGIVAKS
                     MDVEIPSQVSELESLPVLHGDVCRKDE"
BASE COUNT         1440 a          955 c         1308 g         1116 t
ORIGIN      
        1 acggacctat tgtacatgtg ccatcagctt tcctcacata atcttcactt tgaagaggtg
       61 ttccagctaa taagggacag gtacgaagga atctgcaggg accttgacct tgagacccca
      121 ataggccaga tccttgacaa ggtaagggag gacatatcaa agggagcctc aaaggactac
      181 tgtgcaagca ggggagagta cataaacggc cgtatacttg ctgattattt ggagtcggac
      241 tttgtggatg ctgcccaggt cataagattt gacaagaggg gagttttcga tcaggcagag
      301 actgaaagac ttgtgatgga aaggctctca gatgttaaaa gggcagtggt acctggcttt
      361 tatggagctg atggagatgg aaacattgtc accttttcaa ggggaggatc agatgtaaca
      421 gggtcaattc ttgcagatgg agtgggggca aggttatacg aaaactggac ggatgtctca
      481 ggctttctta aggctgaccc caagatagtg aaggaccctt tgcccatcag ggaggtaacc
      541 tacaaggagc tgagggagtt gtcctacatg ggagctccgg ttctccacga ggagtccata
      601 tttcccatca agaacaagaa aattccaata caaatcagga ataccaacaa acctcaggac
      661 cagggaaccc ttatagttaa tgacagctgg gcaggcgttg acccaggaat aatcactggg
      721 attgcaggaa agaaggattt cacagtaatc tcggttgaaa agacccggat gacgggggag
      781 aagggattct tgagaaagct cgtatcagta tttgaggcca aagatgtggc cattgaacac
      841 atgccctcaa gcatagattc aatatcggtt atagtctcgg acaaggaaat gaatggcaag
      901 ctttcaaagc ttatggagga aattaggata tattgcaatc ctgataatat aacagcccat
      961 tccaagatgg cccttttggc tgttgttggc agggggatga tcaacaccaa gggggtttcc
     1021 tcaaggatat tcacagccct ttacaaggac gatataaaca taaggatgat ttcacagggc
     1081 tcaagtgaat tgaacatcct tattgggatt gaaaatgacg attttgaaaa gtccgttagg
     1141 gccatataca atgcattcaa tgaaagggag gtaagctaga tgaaaaaatt caatgttgcg
     1201 gtggtaggag ctacaggact tgttggaaga acaattttac aagtgctcca ggaggaggat
     1261 ttcccaatcg agaacctcta cccaatggca tcggaaaggt ctgaaggaga cctgattcag
     1321 ttccgggaca aggaatacaa ggtaatcaag gtctcggacc aggcatttga tgaagacata
     1381 gatattgcct tctttgcagc tggaggtgca ataagcgaaa ggtatgcacc tgtagctgcg
     1441 gaaaagggga tccgggttgt tgacaacagc tccgttttca ggatgaatcc ggaggttccc
     1501 ctggtggtgc cggaggtgaa tccacaggat atcaaaaagt ccaaggggat aatcgcaaac
     1561 ccaaactgct caacaatcca gtgcatgctg ccacttaagg ccctgcagga ctcctacgga
     1621 attgaaaggg tggtctattc aacctaccag agtgtttccg gatctggaac aaagggtcta
     1681 agggacctgg acgaagggtt aaacgagttc tacccatacc ccatcaaggg taatgtactg
     1741 ccacacatag acgtgtttct tgataatggc tacaccaagg aagagatgaa gatgatagag
     1801 gaaacaagga agattcttca tgatgaggat atgaaggtga ccgcaacagc agtaagggtt
     1861 cctgtgaggt ttgcccacag tgtatcagcc aacgtacaac tttccagaaa ttttgacctt
     1921 gatgtggtta ggaagatgct aggagattat cctggaataa tcctaaagga cgacccagat
     1981 aatggtcagt acccaattcc ccaggatgca gagggaaaaa atgaggtata tgtgggaagg
     2041 gtaagaaggg acttcagcca ggacaatacc ctgaaccttt ggattgtggc cgataacata
     2101 agaaagggag cagccacaaa tgcagttcag atcggcaagc tccttgtgga taattgggag
     2161 gagatgaaat gacaaatatt ggactacttg gatttggaac ggtgggtcaa ggggtttaca
     2221 ggattctcca ggaaaaaggg gaagacctta aggtcctcct tggaagggaa attgaagtaa
     2281 agagcatact tgtaagggac ctctacaagg aacgagaaat tcaggtgccc ttgagcaagc
     2341 ttaccctcaa tcccgaggac attgtgtccg atgaggatat agacataata gttgaggtta
     2401 ccggggatgt ggaccttagc tacaggatca taagcaaggc ctttgaaaga ggcaagcatg
     2461 ttgtcactgc aaacaaggca gttgtgtcgg catattttga ggagttatca cacctggcgg
     2521 aggaaaatgg ggtcttcttt ctttatgagg caagtgtggc cgggggaatt cctgtcctaa
     2581 agcccctcaa ggaccagata aggttaaatg aaataacaag gatccagggg atactgaatg
     2641 gaacctgcaa ctatatcctc acaaggatga cccatgaggg tttggcctat ggggacgtgc
     2701 tgaaggaggc ccaaaggctt ggatacgcgg aagccgaccc ttcatctgat gttgagggga
     2761 tcgatacaat gagaaaacta aggataatgt ccacccttgc cctgggagca tccataaccg
     2821 aggacgacat aatctgcgat ggtatcgaca agatatccga tttggacatt aacctcttaa
     2881 ggaacaaggg tatgaaggta aagctcattg ctgaagccag ggaggtggag ggtggctacc
     2941 aggccatagt tcaaccaaag gcccttccgg tggaatccta ttttgcaggg gtgaatggag
     3001 cctacaatgg catttcaatg gatggcaact attccggcac actgggcttt cagggtccgg
     3061 gagctgggat gtatcccact gcaaatgcag ttttgacaga cgtcattgac tgcattttgg
     3121 aaactcagag aaagatgagt cctcttaggg ggagaaagct tgaaaacagg aatgaggata
     3181 tagctggaag gtattatctt aggatctcag gctatgatgg ttctatggaa aatataaggg
     3241 accaggccga agaggtcctt cttgacaggg acggagaatt tgcctttata acaggacagg
     3301 ttaagctcta ccacctgctc caagggctgg cggagcatga agacaaacct ttttcattga
     3361 tagcaattga acagtaggag gagaaaatga gatattacag cactcgaaac aaggacaatg
     3421 tggtctcttc aatggaggcc ataataaagg ggattgcaga ggatggagga ctttttgttc
     3481 cggaagcctt tccggccctt ccaaaacttg aggacataag gaatttaacc tacaaggaac
     3541 tggccaagct gattcttggt ctgtattttg acgactatcc agaggaggac cttgaagaag
     3601 ctgtcaatgg agcctatggt gacaagtttg gcaacaggga gcctgtgaga cttgtggaca
     3661 agggaggggt gaatttcctc gaacttttcc atggaccaac ccttgccttt aaggatatgg
     3721 ccctaaccat cctcccctac cttatgaggt ctgccctcct aagcaaggga gtggaggaag
     3781 aggttgtggt ccttgcagca acatcgggag atactggaaa agctgccctt gagggtttca
     3841 gggatgtgcc gggagtaaat gtggtcgtct tctatccaga gggaggggtc agccagctgc
     3901 aaaggctgca gatgatcaca caaaagggtg aaaacaccca tgtcctggca atagaaggca
     3961 actttgacga tgcccagaag ggggtcaagg atatctttgg agacctggac tacaacaagg
     4021 agctaaagga ggagggctat gtgctttcct ctgcaaactc cataaacata ggaagactga
     4081 ttccccaggt cgtatactat gtatacgcct acctggacct tctcagaaaa ggcaggattg
     4141 aggtcggtca ggaagtcaac attgttgtgc ccactggaaa ctttggcaac atactggctg
     4201 cctactacgc caagatgatg ggccttccag ttgacaggct gatatgctca tcaaacgaca
     4261 acaaggtcct gactgacttc tttgaaaccg gtacctatga caggaggaga gaccttcttc
     4321 taacatcctc gccatcaatg gacatactgg tatcaagcaa ccttgaaaga tttctcttcc
     4381 acatatcagg gaatgaccac caggctgtaa gggagatgat ggaagacttg tccagggaag
     4441 gatacttcaa gtggtcagat ttcagtcggg aagaaatcta cggagaattt gcggatgagg
     4501 atgaggtttc ttcagccata agggatcttt atgaaagtga aggctatgta atggatcccc
     4561 atacggctgt tgcctacagg gtttacaaaa agtaccttaa ggagactgga gactcaaagc
     4621 ctgcaataat tgcatcaaca gccagtccat tcaagtttcc cgaaaaggtt atctcatcca
     4681 tgggagagga agtcccacag gatcttttta ctggaatcgg aattgttgct aaatcaatgg
     4741 atgtggaaat accaagtcag gttagtgaac tggaaagcct tccggttctt catggggatg
     4801 tatgcaggaa agatgagat
//
LOCUS       DASRQF010000299         3187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_423542, whole genome shotgun sequence.
ACCESSION   DASRQF010000299 DASRQF010000000
VERSION     DASRQF010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423542"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..271)
                     /locus_tag="VFD79_08020"
     CDS             complement(59..271)
                     /locus_tag="VFD79_08020"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HZH94037.1"
                     /translation="MEGFVITRELVMMLMPLILLQVGMFMFCATKIYREGVENLNRWI
                     WILICLFVNLVGPIAFLIAGRKREYR"
     gene            complement(313..564)
                     /locus_tag="VFD79_08025"
     CDS             complement(313..564)
                     /locus_tag="VFD79_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH94038.1"
                     /translation="MKVVTLLMTAVTVQITCEGCRILLKEYKLTERRDIMGKLAKILI
                     VIAIALAMIKGFGELFYFSNAEVTEEGTFQEEPGRRDKL"
     gene            complement(591..1226)
                     /locus_tag="VFD79_08030"
     CDS             complement(591..1226)
                     /locus_tag="VFD79_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZH94039.1"
                     /translation="MNRIKVLLADDQRIILDGLKALLEMNTRISVVDMAENGASAYEK
                     TLEIKPDVVLMDIRMPKMDGVEATRLIKRDAPETVVIMLTTFDDDEYIIKAMTYGASG
                     YLLKDIGSDKLIEAIVDGMKGSIILPGKVAAKITSRLREEGSSGITTGDFTQRELEII
                     SLMMKGMTNREISEDLYLSIGTVKNYISQIYLKIEVNDRAKAVLQLKKMGF"
     gene            complement(1219..2619)
                     /locus_tag="VFD79_08035"
     CDS             complement(1219..2619)
                     /locus_tag="VFD79_08035"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZH94040.1"
                     /translation="MEVSRFLPIIIPGIIVQIAIQAYFIRHALRNGELSRAKRRAYAL
                     AIAVLNLPAAAVYLFNNRRTEKRMARDFEGVDVDPNIKQGIFVMLVIAYEIFSLRIIA
                     DNVENPYQGLLAGLLAYCFVIMVINNLFIDENSGILYYILPITQLVIAIPIEYLDNSY
                     FAQFIVLIVTASIINRFPFRLARPYTMAAFCAYWIGGVAKALRFNGLDGMDEIISYLY
                     INTLLFVLVIAAFYTLKRQLLTNNQLSAALERVQDQAEQLREMGAISERNRITAEIHD
                     KVGHTLTSAVISIENAEKLFGDQPEEALEKLSLAKDQVKRGLGEIRLSVRTIRKGEVG
                     NFPAALGELQEEMLQNTGMEISIIMDVKEAIPPILQGVLLGSIKECCTNSLKHGKANR
                     ADILFQDFRERLCMTYSDNGVGTDEIVYGFGLTAMKERVLGVGGTMNTESGPGEGFSV
                     EITLPIGRAVGGEDHE"
     gene            2741..>3187
                     /locus_tag="VFD79_08040"
     CDS             2741..>3187
                     /locus_tag="VFD79_08040"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZH94041.1"
                     /translation="MGAKKGWLWTKIDSDYWSEPSEEIYYLGSRWEDMGFRKFLDLGC
                     GIGRHSLHMAKLGFDVTAYDLSDTGLQLLRDKASKMELSIHTVKGDNERLTFNDSSFD
                     CLLAYHSLYHSDSEGMGRIVSEMHRVLGSGGEGYLTLISKYHPDYLR"
BASE COUNT          810 a          815 c          639 g          923 t
ORIGIN      
        1 tccatcccat tgagtacctt ttttcccccg aagctcttgt ggatattcct tgcggttatc
       61 atctgtactc tctctttctt ccagctatga gaaatgctat gggcccaaca aggttgacaa
      121 aaaggcatat gagtatccaa atccacctgt tgagattctc aacaccttcc ctgtaaatct
      181 ttgttgcgca aaacatgaac atgccaacct ggagtagaat caagggcata agcatcatca
      241 ccagttccct ggttataaca aatccttcca tttgcaaaac ctccatccaa caatcacctt
      301 ctcatgcgat cactaaagtt tgtcccttct tcccggctcc tcttggaagg tcccttcttc
      361 agtaacctct gcattgctga agtagaacaa ctcgccaaat cccttgatca ttgccagggc
      421 aatagcaatt actataagta tcttcgccag cttacccata atatccctcc gttccgttaa
      481 tttgtattcc ttcaacagta ttctacatcc ctcacaggta atctgaacag ttactgcagt
      541 cataagtaga gtaactactt tcaaaaaaaa gaagaggtcg ccctcttccc ttaaaaaccc
      601 atctttttca gttgcagaac cgccttggcc cggtcattga cttcaatctt caggtagatc
      661 tggctgatat agttctttac agttccaatg gatagataca ggtcctcaga tatctccctg
      721 ttggtcatgc ccttcatcat cagggaaatt atctcaagct ccctttgagt gaaatcacct
      781 gtggtaatcc ctgaagaacc ttcttccctt agtcttgatg taatctttgc ggcgaccttg
      841 cctgggagga tgatgctgcc cttcatgcca tccactattg cttctatcag tttgtcactg
      901 ccaatatcct taagcaggta gcctgatgca ccataggtca tggcttttat tatatactca
      961 tcgtcgtcaa aggttgtcag catgatgacc acagtttcag gagcatccct cttgatcagt
     1021 cgggtggctt ccaccccgtc catcttcggc atccggatat ccatcaagac aacatcgggc
     1081 ttgatttcaa gggttttttc ataggcactg gcaccatttt cagccatgtc caccacactt
     1141 attcttgtgt tcatctccag tagggccttc aacccatcca atattatcct ttggtcatca
     1201 gccagtaaaa ccttgatcct attcatgatc ctcacctcca acagccctgc caatgggtag
     1261 ggtgatctca acagaaaatc cctctccggg accactttcc gtattcatgg taccaccaac
     1321 tcccagtacc ctctccttca tggcggtaag tccaaatcca tacactatct catcggttcc
     1381 aactccattg tccgaatatg tcatgcacaa cctttccctg aagtcctgaa acagaatgtc
     1441 cgccctgttt gccttcccat gcttaagact gtttgtgcag cactccttta tggaacctag
     1501 gaggacaccc tgtagaatcg gtggtattgc ttcctttaca tccattataa tcgagatttc
     1561 cattcccgtg ttttgtagca tttcctcctg tagttccccc agtgctgctg gaaaattccc
     1621 cacctcccct ttccggattg tcctgactga cagccttatt tcaccgagcc cccttttaac
     1681 ctggtcctta gccagggaca gcttttcaag agcttcctcc ggttggtcgc caaagagctt
     1741 ctcagcattc tcaattgaaa ttacagcact tgtcaaggtg tgtccgacct tgtcatgaat
     1801 ctcagcagtg atcctgttgc gttctgagat tgctcccatt tccctcagct gttctgcctg
     1861 gtcctggacc ctttcaaggg ctgcacttag ctggttgttt gtaagcagct gacgcttgag
     1921 agtgtagaag gctgctatca caaggacaaa gagcagggtg tttatataca ggtagctgat
     1981 gatctcgtcc atcccatcca gtccattgaa cctgagggcc tttgcaacac ctcctatcca
     2041 gtaggcacag aaggctgcca tggtgtaggg ccttgcaagt ctgaagggaa acctgtttat
     2101 tatactggct gtcactatta agacgataaa ctgggcgaag tatgagttgt ccagatactc
     2161 aatcggtatg gcaatgacca gctgagtgat gggtagaatg tagtatagga ttccagaatt
     2221 ttcatctatg aaaaggttgt ttatcaccat tatgacaaag caataggcca gcaggccagc
     2281 aagtaggcct tgatatggat tttcaacgtt atcggcaata atccttaggg agaaaatttc
     2341 ataggctatc acaagcatga caaatatccc ctgcttgata ttgggatcaa catccacacc
     2401 ttcaaagtca cgtgccatcc ttttttccgt cctgcgattg ttaaacaggt agacagcagc
     2461 agcaggaagg ttgaggacag caatggccaa ggcgtaggct ctccgcttag cccttgaaag
     2521 ttcaccattt ctcaaggcat gcctgatgaa gtatgcctga atggcaatct gaacaatgat
     2581 tccaggtatt attatcggca agaatctact cacttccata caactacctt cctttcttcc
     2641 actccatatt gacaaaggat ccaccttgat atatctttat tatacgaaat attctgataa
     2701 caataaagca tgcgctatta ttttttcaaa ggaggtactt atgggtgcaa aaaaaggttg
     2761 gctatggaca aaaatagact ctgactactg gtctgaacca tcagaggaga tctattatct
     2821 gggaagtaga tgggaggata tgggttttag aaaatttctt gatcttggat gtggcatagg
     2881 gagacattct ctacacatgg ccaagctggg ctttgatgtg acagcttacg acctttccga
     2941 tacggggctt cagctcctaa gggacaaggc ctccaaaatg gaactatcta ttcatacagt
     3001 caagggcgat aacgagagac tgacattcaa tgactcctct tttgattgtc tccttgccta
     3061 tcactccctc taccactcgg attcggaggg gatgggcagg attgtttccg agatgcacag
     3121 ggtgctgggg agtggtggag aaggctacct gaccctgatt tcaaagtacc atcctgatta
     3181 tctgagg
//
LOCUS       DASRQF010000300         7282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_547844, whole genome shotgun sequence.
ACCESSION   DASRQF010000300 DASRQF010000000
VERSION     DASRQF010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 7282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7282
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547844"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VFD79_08045"
     CDS             complement(<1..402)
                     /locus_tag="VFD79_08045"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HZH94042.1"
                     /translation="MAYPRITIDTEKLTNNVRAVMKLVAPFNISVAGVTKGFSANPEI
                     AKAYVDGGVNYLADSRLQNLKKLKDLDIPKLMLRLPMVSEADELVRYADVSLNSELET
                     IKALNGAAQKNNKVHDVIIMVDLGDLREGYFH"
     gene            complement(418..1782)
                     /locus_tag="VFD79_08050"
     CDS             complement(418..1782)
                     /locus_tag="VFD79_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmL-related ornithine degradation protein"
                     /protein_id="HZH94043.1"
                     /translation="MKINLLVAEIGSTTTVVNAFNQIETDPVFLGQGQGATSVLQGDV
                     TIGLQEAIDDLCRNLKVDKVDYDELIATSSAAGGLRMTVHGLVYDMTVRAAKEAALGA
                     GANIKYITAGKLRKSDLRKIKETNPNIILIAGGVDYGERDTALNNAELIAQLNLGIPV
                     IYAGNVENHEDIRDIFEGTHNELYVVENVYPKIDELNIEPTRKVIQSVFEKHIIHAPG
                     MEKIREMVTGNIMPTPGAVMEASQVLYDEIGDLMTIDVGGATTDVHSVTEDSEEISRI
                     ILSPEPMAKRTVEGDLGVFVNMRNIVDMVGEDKLMEELDLDEPALDNLINHHKPIPET
                     DLEKRFVEVLAEHATLTAVKRHSGRLKHIYGPSGKKTIAEGKDLTGVRYIIGTGGALT
                     RLPGRTEIIEKVPMSNNGTELLPNREAEILIDNHYIMASLGVMSKKYPEIALDLMMKS
                     FGLR"
     gene            complement(1772..3988)
                     /gene="oraE"
                     /locus_tag="VFD79_08055"
     CDS             complement(1772..3988)
                     /gene="oraE"
                     /locus_tag="VFD79_08055"
                     /EC_number="5.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine 4,5-aminomutase subunit OraE"
                     /protein_id="HZH94044.1"
                     /translation="MELKPNEKLNIKELLKDIENYRPKRRGWHWREGVGEKRQIGKFE
                     YYDTSENLKQSQPLPASKYFENIDPQPQPVITTEIASGRFEDDIRRMRMAAWHGADHL
                     MVIRTAGQSHFESLIEGTPQGVGGIPISRKQVRAQRKALDLIEEEVGRPINYHSYVSG
                     VAGPEIAVMFAEEGVNGAHQDPQYNVLYRNINMIRSFVDAAEAKKVMTWADIAQIDGA
                     HNANATAREAWKVMPELMVQHAINSIFSVKVGMKKGNICLSTVPPTAPPAPSMRLDLP
                     YAVALRDLFREYRMRAQQNTKYMESSAREATVTHVLNLMISQLTSADIQSTITPDEGR
                     NVPWHIYNIEATDTAKQVFNGLDGLMSMIQLKRHDSELGDKVRELKERAILFMEEMLE
                     IGGYFKAVQDGFFVDSGYYPERNGDGIIRKIDGGVGEGTVYERDQDYMAPVTAHFGYN
                     NVAQYGEEYVENPSKLIGGSTLEDPDKIIFIDELDESDNVNARLEEVKDLIGTTKLKP
                     EMEWLGDGIILLTMFLPTDVRTAEFAALEIGKKMNLHDCEVIHKEIMQEAEGTRIELK
                     GKVDFSIDTSELVIPPKPEEMSDDQIREDIEDKPMKVVAATVGEDEHSVGIREIIDIK
                     HGGIEKYGIECHILGTSISVEKLVDAAIEINADAILTSTIISHDDIHYKNMKKLHQYA
                     IERGVRDKMIFIAGGTQVSNEQAVANGMDAGFGRGTKGSHVATFLVKKRRDMANED"
     gene            complement(3988..4353)
                     /locus_tag="VFD79_08060"
     CDS             complement(3988..4353)
                     /locus_tag="VFD79_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HZH94045.1"
                     /translation="MKRADDFQERRKHLADLSEDQLEAKFWELAEKLVDPIVELGYKN
                     TSPSIERSVLLRMGFSSVEAKGIVDNAIDRGLIGKGAGHIVYKLADKKGISIREAGLL
                     LVEGDSWDEVKPLFKGGAK"
     gene            complement(4353..5786)
                     /gene="ortB"
                     /locus_tag="VFD79_08065"
     CDS             complement(4353..5786)
                     /gene="ortB"
                     /locus_tag="VFD79_08065"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtB"
                     /protein_id="HZH94046.1"
                     /translation="MTRDRSFSAVMARKPEIMKSSVGIDYDIFESGRIAFDYERMMKE
                     TGYSLQEIEEIQKETTVGNTPLYELRNITKLARKYAGPGKGARIFIKDEASNPSGSFK
                     ARRAATAVYHAQKLGYKGVIAATSGNYGAAVASQAAMRGLKCIIVQECYDSKGVGQPE
                     IIEKARACEAYGAEVVQLSVGPELFYSFLLLLEDTGYFNASLYTPFGIAGVETLGYEL
                     SMEFREKEGRDPDVVVVTNAGGGNLTGTARGLIKAGALDTKVIGASVSLAGLHMASDE
                     QFNKKSFTTGHTGFGIPFATWPDRSDVPRSAARPLRYMDRYLTVNQGSVFYMTELLAQ
                     VEGLERGPAGNTALAAAFVLAQEMDEDQILVVQETEYTGAGKHINPQLTFAKENGIKI
                     LKGDPSQEVPGENIILPENPGKLCVEELDIEKTKKSYIRNCIKNTEVDSVNREDLDFL
                     VADTKSNESFVRETLEELGVKVETEER"
     gene            complement(5779..6090)
                     /gene="ortA"
                     /locus_tag="VFD79_08070"
     CDS             complement(5779..6090)
                     /gene="ortA"
                     /locus_tag="VFD79_08070"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtA"
                     /protein_id="HZH94047.1"
                     /translation="MDAKKGDWVRIHKIVLEVGERAPNIPEDTQNVPLEMWDKGFLLN
                     DEANIGDQVEIETYIGRKVTGNLLEVNPFWDHDYGKAVPELLYIGRQARDLLEEGESN
                     D"
     gene            complement(6198..7247)
                     /gene="ord"
                     /locus_tag="VFD79_08075"
     CDS             complement(6198..7247)
                     /gene="ord"
                     /locus_tag="VFD79_08075"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-diaminopentanoate dehydrogenase"
                     /protein_id="HZH94048.1"
                     /translation="MRKENVKVIIWGLGAMGSGMADMLLKKKGVEIVGAVGRGNKLGK
                     SMYDFIKTERGDRPDVIIGTPEEVITEGAADVVLTATDSYTKTAFERLKFILEKKINV
                     VSSAEEMAYPQAQSPEEAKMLDEIAKANGVSILGTGINPGLIMDLLVVVMTGCCETVD
                     SVTARRVNSLSPFGPAVMEEQGIGITKEEFEEGVRTGHLAGHVGFPESIHMIADAIGW
                     KVDKVEQSMEPIMTDVDRKSPYGFAKAGNVAGCAMKGFGYVDGEMKIEMDHPQQIEPE
                     MVGVQTGDYVIIKGNPNINLVNSPEVPGGIGTIAMCVNMIPHIINARPGLKTMIDLPV
                     PRAIMGDMREMIEVE"
BASE COUNT         1590 a         1937 c         1368 g         2387 t
ORIGIN      
        1 atggaagtat ccttccctca ggtctcccag gtccaccatt attataacat catgaacctt
       61 gttgttcttt tgggctgctc catttaaggc cttgattgtt tccagctctg aattcaatga
      121 tacatctgca tacctgacaa gctcatctgc ctccgatacc attggtagtc tgagcataag
      181 cttggggatg tccaggtcct taagcttttt cagattctga agtcttgaat cggcaagata
      241 gtttacccca ccgtccacat aggcctttgc gatttcagga tttgcagaaa atccctttgt
      301 aacaccagca actgaaatat tgaatggtgc caccagcttc attactgccc ttacgttgtt
      361 tgtcaatttt tcagtgtcta tggtaattct tggatatgcc atagcattcc tcctgttcta
      421 tctcaatcca aagctcttca tcataaggtc aagagcgatt tctgggtatt tctttgacat
      481 tactcccagg gatgccatta tataatggtt atcaatcagg atttcagcct ccctgttggg
      541 caaaagttca gtcccgttgt tggacatcgg aaccttttca attatctctg ttctccctgg
      601 tagccttgta agtgctccac ctgtcccgat tatatatctc acccctgtaa ggtcctttcc
      661 ttcagcaata gtcttctttc cactgggtcc atagatatgc ttgagcctgc cgctatgtct
      721 ctttacggct gtaagtgttg catgctctgc aaggacctcc acaaacctct tttcaaggtc
      781 agtttccggt attggcttgt ggtggttgat aaggttatca agtgccggtt cgtcaaggtc
      841 caattcctcc atcagcttgt cctctccaac catgtccaca atgtttctca tgtttacaaa
      901 aactcccagg tctccctcca ctgtcctttt tgccattggc tcaggactta ggattattct
      961 tgagatttct tcgctgtcct ctgtcactga gtgaacatca gtggtggctc cacctacatc
     1021 tattgtcata aggtctccaa tttcatcgta aaggacctgg gaagcctcca ttacagcacc
     1081 tggggttggc atgatgtttc ctgtgaccat ttccctgatc ttctccattc caggagcatg
     1141 gatgatgtgc ttttcaaata cggactggat gactttcctg gttggttcaa tgttcagctc
     1201 gtcaattttg ggatatacgt tttcaacaac ataaagctca ttgtgggtgc cttcgaaaat
     1261 atccctgata tcctcgtggt tttccacgtt acctgcatat attacaggta ttcctagatt
     1321 caattgggca atcaactcgg cgttgttaag agcagtgtcc ctttctccgt agtcaacacc
     1381 acctgcaata aggatgatgt ttggattggt ttccttaatt ttcctcaggt ctgattttct
     1441 cagcttacct gcagttatat acttgatgtt ggctcctgct ccaagtgcag cctccttggc
     1501 cgcccttacg gtcatgtcat agaccagacc gtgtacggtc atcctaagcc cccctgcagc
     1561 ggagcttgtg gctataagct cgtcatagtc caccttgtcc accttcaggt ttctgcaaag
     1621 atcatcaatg gcttcctgaa gtccaattgt tacgtctcct tgaaggacag aggttgcccc
     1681 ctgtccctgt ccaagaaaaa ccggatctgt ttcaatctgg ttaaatgcat ttacaactgt
     1741 tgttgtactt cctatttcag ctacaagaag attaatcttc attggccata tcacgtctct
     1801 tcttcactag gaaagtggct acatgtgaac ccttggttcc acggccaaat cctgcatcca
     1861 ttccatttgc aactgcctgc tcgtttgata cctgagttcc acctgcaatg aagatcatct
     1921 tgtcccttac gcctctctca attgcgtact ggtgaagctt cttcatgttc ttgtagtgta
     1981 tgtcgtcatg ggaaatgatt gttgaagtca agatcgcatc tgcattgatc tcaattgcag
     2041 catcaactag cttttccact gatattgaag ttcccaggat gtggcattct ataccatact
     2101 tttcaattcc accatgcttg atatctatga tctcacggat tccaacagag tgctcgtctt
     2161 ctcccacagt tgcagcaaca accttcatag gcttgtcctc aatgtcctct ctgatctggt
     2221 catctgacat ttcctcaggt ttaggtggaa ttacaagctc gctggtatcg attgagaagt
     2281 caaccttacc ctttagctcg attctcgtac cttctgcctc ctgcatgatc tccttgtgga
     2341 ttacctcaca gtcatgcagg ttcattttct taccgatctc aagagcagca aactctgcag
     2401 tccttacatc tgtcggcagg aacattgtca ataggatgat cccatcacca agccattcca
     2461 tttcaggctt gagcttggtt gttcctataa ggtccttaac ttcctcaagt cttgcattaa
     2521 cgttgtctga ctcgtcaagc tcgtcaatga agattatctt gtccggatcc tcaagagttg
     2581 agcccccaat aagctttgat gggttttcaa catactcttc cccatactgg gccacattgt
     2641 tgtatccaaa atgggctgtg actggagcca tgtagtcctg gtccctttca tagacagtac
     2701 cttctccaac tccaccatca atctttctta tgatcccgtc tccatttctt tcaggatagt
     2761 atcctgagtc tacgaagaag ccatcctgga cagccttgaa gtagcccccg atttcaagca
     2821 tttcctccat aaagagaata gccctttcct taagctccct taccttgtct ccaagctcgc
     2881 tgtcatgtct cttgagctga atcattgaca tcagtccgtc aagtccattg aatacctgct
     2941 tggctgtgtc agttgcctcg atgttgtata tgtgccaagg cacgtttcta ccttcatctg
     3001 gtgttattgt ggattgaatg tcagctgaag taagctggga aatcataagg ttcaacacgt
     3061 gggtaacagt ggcttccctt gctgatgact ccatgtactt tgtattctgc tgggctctca
     3121 tcctgtattc cctgaaaagg tctctcaagg caactgcata tggcaggtca agtctcatgc
     3181 ttggtgccgg tggtgcagtt ggaggaactg ttgaaagaca gatgttgccc ttcttcattc
     3241 caaccttcac ggagaatatt gagtttattg catgctgaac catcagttca ggcattacct
     3301 tccatgcttc cctggcagtt gcatttgcat tgtgtgctcc atcgatctgt gctatgtcag
     3361 cccaggtcat aaccttcttt gcctctgctg cgtcaacaaa tgacctgatc atgttaatgt
     3421 tcctgtaaag aacgttatac tgtggatcct ggtgggctcc gtttacgcct tcttcagcaa
     3481 acattactgc tatttcagga cctgcaactc cagatacata tgagtggtag tttattggtc
     3541 ttcctacctc ttcctctatc aggtcaagag cctttctctg agcccttacc tgcttacggc
     3601 ttatgggtat tccccctact ccctgtggag taccttctat aaggctctca aagtgtgact
     3661 gtccagctgt ccttataacc ataagatggt cagcaccatg ccaggcagcc atcctcattc
     3721 ttctgatatc atcctcaaac ctgcctgaag cgatctctgt tgtaatgaca ggctgtggtt
     3781 gtgggtcgat gttttcaaag tacttggatg ctggaagtgg ctggctctgc ttaaggttct
     3841 ctgaagtgtc gtagtattcg aatttaccga tctgtctctt ttcgcccact ccttctctcc
     3901 agtgccatcc tcttctcttg ggcctgtagt tctctatatc cttcagtagt tcctttatgt
     3961 tcaatttctc atttggttta agctccatta cttggcacct cctttaaaca agggttttac
     4021 ctcatcccat gaatctcctt caacaagaag cagaccagct tcccttatgc ttattccctt
     4081 cttgtcggcc agcttgtata cgatgtggcc agcacccttg ccgatcaggc ctctgtcaat
     4141 tgcattgtca acaatacctt tagcctcaac acttgagaag cccatcctta aaagaacgct
     4201 tctctcgatt gaaggacttg tatttttgta gcccagctca acaattggat ccacaagctt
     4261 ttctgcaagt tcccagaact ttgcctccaa ctggtcttct gaaagatctg ccaggtgttt
     4321 tcttctctcc tgaaaatcat ctgctctctt cattatcttt cctccgtttc aacttttact
     4381 cccaattcct ccagcgtttc cctgacgaaa gattcatttg acttggtgtc tgccaccagg
     4441 aagtcaaggt cttccctgtt tacactgtca acttccgtgt tcttgatgca gtttctgata
     4501 tatgacttct tggttttttc tatgtccagc tcttccacac aaagttttcc aggattctca
     4561 ggtaggatta tattctcccc tggaacctcc tgtgaaggat ctcccttaag gatcttgatc
     4621 ccgttttcct ttgcaaaggt caattgggga tttatgtgct ttcctgcccc tgtatactct
     4681 gtttcctgta caacaagaat ctggtcctca tccatttcct gggccaatac aaatgccgca
     4741 gccaaggctg tgtttccggc tggtcccctt tcaagtccct caacctgtgc aagaagctct
     4801 gtcatgtaga agactgaccc ctggtttact gtaaggtatc tgtccatgta tctaagaggt
     4861 cttgcggcag atcttggaac gtcggacctg tctggccagg ttgcaaatgg gatcccgaat
     4921 ccagtatgac ctgtggtgaa tgacttcttg ttgaactgct catcacttgc catgtggagt
     4981 ccagccaggc ttacgctggc accgattacc ttggtatcca gggctcctgc cttgatcaac
     5041 ccacgtgcag ttcctgtaag gtttcctcca ccagcatttg ttacaaccac tacgtccgga
     5101 tctcttcctt ccttctccct aaattccatt gacaattcat agcccagggt ctcaacacct
     5161 gctattccaa atggtgtgta gagggaagca ttgaaatatc ccgtatcctc caaaaggagc
     5221 aggaatgaat agaaaagctc gggtcccact gaaagctgga ccacttctgc cccataggct
     5281 tcacaggctc ttgccttttc aattatttca ggctggccca ctcccttgga atcgtagcac
     5341 tcctgaacaa tgatgcactt gaggcctctc atggcagcct gggatgcaac tgctgctccg
     5401 tagtttccag atgtggccgc tataacacct ttatacccca atttctgtgc gtggtaaacc
     5461 gctgtagcag cccttctggc cttaaagcta ccagacgggt tgctggcctc atcctttatg
     5521 aaaattctcg ctcccttacc tggaccggca tattttcttg caagctttgt aatgtttctc
     5581 aattcataaa gtggtgtgtt gccaactgtt gtctcctttt gaatctcttc aatctcctga
     5641 aggctgtaac cggtttcctt catcattctc tcatagtcaa aggcaatcct gccagactcg
     5701 aatatgtcgt agtctatacc cactgatgac ttcattattt caggttttct tgccattacg
     5761 gcgctgaagc ttctatccct agtcattgct ttcaccttcc tccaataggt ctcttgcctg
     5821 tcttccaatg tataggagtt caggcaccgc ctttccgtaa tcatggtccc agaatggatt
     5881 aacctcaagg aggttaccag taacctttct tccaatgtag gtttcaatct caacctggtc
     5941 tccaatattt gcctcatcat tcaataggaa tcccttgtcc cacatttcca agggtacatt
     6001 ttgagtatcc tctggaatat taggtgccct ttcccctacc tcaaggacaa tcttatggat
     6061 tcttacccaa tcgccttttt ttgcatccat ttctcttcct ccctttctac ctatccttat
     6121 ggaagaatga gtagatacaa tatctactca tcttctatat gtttcagatt cttaacaata
     6181 ttgttgcatt tctttattta ctcaacctca atcatttccc tcatgtcacc catgattgct
     6241 cttggcactg gtaggtctat cattgtctta agaccaggtc tagcattgat gatatgtgga
     6301 atcatgttta cacacattgc tattgttcct attcctcctg gtacctcggg tgagtttacc
     6361 aggttgatgt ttgggttacc cttaattatt acgtagtcac ctgtctgtac ccctaccatc
     6421 tcaggctcaa tctgctgagg atgatccatt tctatcttca tttcaccatc tacatacccg
     6481 aagcccttca ttgcacatcc tgcaacgttt ccagcctttg caaagccata tggtgatttc
     6541 ctgtctacat ctgtcatgat aggctccatt gattgctcta ccttatcaac cttccaacct
     6601 attgcatctg ctatcatgtg aattgactca gggaagccta catgacctgc cagatggcct
     6661 gtcctgactc cctcttcaaa ctcctctttg gtgattccaa tgccctgctc ctccattact
     6721 gcaggaccga atggtgaaag gctgtttacc cttcttgcag taacactgtc aactgtctca
     6781 caacagcctg tcattactac cacaagcagg tccatgatca gtccaggatt gattccagtt
     6841 cctaggattg atactccgtt tgccttggcg atctcatcca gcatcttggc ctcttcaggt
     6901 gactgtgctt gtggatatgc catttcctct gctgatgata ctacgtttat cttcttttca
     6961 agtatgaact taagtctctc aaatgctgtt tttgtgtatg aatctgtggc agtaagtact
     7021 acatcagcag ctccctcagt tattacttcc tctggtgttc caataattac atcgggtctg
     7081 tcgcctcttt cagtcttgat aaagtcatac atgctcttcc caagcttgtt tcctcttccc
     7141 actgctccta caatttcaac gcctttcttc ttaagcagca tgtctgccat tccgcttccc
     7201 attgcaccta gtccccaaat gattaccttt acattttctt ttctcattgt acacctccat
     7261 ggaatttgtc aatactaact tc
//
LOCUS       DASRQF010000301         6676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1294370, whole genome shotgun sequence.
ACCESSION   DASRQF010000301 DASRQF010000000
VERSION     DASRQF010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6676
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294370"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      628..742
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            824..1432
                     /locus_tag="VFD79_08080"
     CDS             824..1432
                     /locus_tag="VFD79_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HZH94049.1"
                     /translation="MYTLSKEAWNTRMMVKISVLGVISFILMFFEIPLVWLAPPFIKI
                     DISDLPALIGAFAMGPMVGVLIQLLKNVLNVVIEGTTTGGIGELANFVVGSAFAYTAG
                     FVYFKKKTFGRAVAGLVLGTIVMTVVISIANYYVMFPFYAKLFGWPIENLVEMGTAVN
                     SNIVDMKTMIIYSIVPFNLLKGTILTAITILIYKKVSPLLHN"
     gene            1560..2495
                     /locus_tag="VFD79_08085"
     CDS             1560..2495
                     /locus_tag="VFD79_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH94050.1"
                     /translation="MIDKLNEEIYEMKKRLREYNKLKSFRESIQEELTHMVKARGDLL
                     NQYDKELKDVEMLEKVGVRSLFLSITGKKEESLDREKEELLRAKASYEECQTNIEDLQ
                     KDLAEADRLLAKFGDIEGNFNGLLKEKEDLLIQSGGSQGKALGERLIRADQLQVDIRE
                     IREAIAAGRSTLESLKGVREKLDSAKGWGTWDLLGGGLISNIAKHSAIDEANNRAREV
                     QHQLKAFRKELSDVNEFTDIEVNISAFASFADFFFDGLIADWFVQSKINDSIGHVEDA
                     NRSIEGIVQNLQNRLGGLEKELQDTKEEIGNILRN"
     gene            2603..3058
                     /gene="tsaE"
                     /locus_tag="VFD79_08090"
     CDS             2603..3058
                     /gene="tsaE"
                     /locus_tag="VFD79_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HZH94051.1"
                     /translation="MRIEIKGIEETDIFGRRLGELVQPGDVICLNGDLGAGKTTLSKS
                     VGLGLGVEDYITSPTFTIINEYQGRLPLYHFDVYRLKDGEELYDLGVEDYFYGDGVCL
                     IEWAQNIQDYLPQDRLELWIYGTDNPNERIVELKAFGDRSEFLAKELIR"
     gene            3055..3750
                     /gene="tsaB"
                     /locus_tag="VFD79_08095"
     CDS             3055..3750
                     /gene="tsaB"
                     /locus_tag="VFD79_08095"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HZH94052.1"
                     /translation="MKILGLDTSTMMTTCAVMEDETLLGEFSLSLDMSHSEALVPMIG
                     ELMDNLGMKIGDMDGFAVSKGPGSFTGLRIGLATAKGFAHVTGKPIVGVSSLEVLADN
                     MYGHEIVVPMMDARRDRVFTAIYTMDNDQWLPVLKPDALPVEDLIQIINRDHERVTLV
                     GDGAVRYRSEFLEGLRGKVLFAPGSLNLSRAASVCRLGMLRLLNGQLDDMFTMAPEYL
                     RDSQAQRMQKKTR"
     gene            3753..4205
                     /gene="rimI"
                     /locus_tag="VFD79_08100"
     CDS             3753..4205
                     /gene="rimI"
                     /locus_tag="VFD79_08100"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HZH94053.1"
                     /translation="MKIILREMTEKDIDEVLEIERASFSTPWSRESFQKEVTENALAV
                     YIVAEVDMELVGYGGLWKILDEGHITNVAVKKESRGKGIGDALVMGIVDYCDRTGIPN
                     ITLEVRESNMVARNLYKKHGFLDAGRRPGYYSDNNEDAVIMWRKKSQR"
     gene            4211..5218
                     /gene="tsaD"
                     /locus_tag="VFD79_08105"
     CDS             4211..5218
                     /gene="tsaD"
                     /locus_tag="VFD79_08105"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005955985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HZH94054.1"
                     /translation="MLTLAIETSCDETSCGVVKDGREVLSNIISSQIEIHREYGGVVP
                     EIASRKHIEGIDVIVQEALDKAKVGFEDIDFISVTQGPGLVGALLVGLSTAKAMAYGL
                     DIPIVGVNHIEGHICANFIEHKDLEPPFTCLIVSGGHSYLIDVLDYSSYRLYGRTRDD
                     AAGEAFDKVARALGLPYPGGPFIDRLALEGDGSAIDFPRVFLEDDSYDFSFSGLKTAV
                     LNYLNQLRQRNEEPILADVAASFQQAVLEVMVEKSFRLAKETGRDKIVIAGGVAANKG
                     LRDMMEKRGRKENVDIFYPSVQLCTDNAAMIGSAGYYNYINGRVSDLHMKVMPNLGFL
                     D"
     gene            complement(5361..>6676)
                     /locus_tag="VFD79_08110"
     CDS             complement(5361..>6676)
                     /locus_tag="VFD79_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HZH94055.1"
                     /translation="GAIGWLEKFLRDYRGSSLIISHDRYFLDNLANRIFHMENRGLKS
                     YSTDYTGFMVQRKKELEIMRRQYDNQQKEIKRQEEIIQRFMNYGGERYIKQARSRQKM
                     LDRIKLLPKLSESKKASFKFDPETRSGRDVLHGEGICKSFGDLELLRDISLDIYRGEK
                     VGLIGANGIGKTTLFRILLGQVDLDQGIVKLGQHVYPGYFDQEMTDLSLDKTVLDEIW
                     DEYPLLDHYQIRTYLSQFLFVGDDIFKEIKELSGGEKARVALLKLMLSKSNFLLMDEP
                     TNHLDIDSKEVLEDALKNYSGTIFVISHDRYFLNQVATKIFELTEDGVTEFMGNYDYY
                     VEKKAFLSLDLEEEGEEKTKTQLKHEKRKEKDQLQFERERKRTIRNLEENISALEEKI
                     EMIDKKLSDPEMYNDHEVVLELSKERENLQNRLDATYAQWMEFGE"
BASE COUNT         1945 a         1275 c         1685 g         1771 t
ORIGIN      
        1 acagtgatac cctctcaaca aaatatatca aaatcataga ttttggatat tttagttgcc
       61 gggcacttac aatgaacagt gaaagtaagc tgtcattctg aactagagtg aagaatctgg
      121 gtagttaaga agtaagaagt aagaggtaag aggtaagaag taagaggtaa gaaagaacag
      181 tgaacagtga acagtgatac cctctcaaca aaatatatca aaatcataga ttttggatat
      241 tttagttgcc gggcacttac aatgaacaat gaaagaaagc tgtcattccg agcgtagtcg
      301 aggaatctag gtagttaaga agtaagaagt aagaagttga aagtagaaaa tggaaagaaa
      361 ggagagatcc ctccatttca gtcgggatga cagctagaac gtcatctcaa gcttgtcgag
      421 agatctcaga cccttaagct ggataggagc aaatatgatt gcttgtgccc ggcgatagat
      481 ttttaaaaaa tctatgattt ttatagaaat catcgagagg gtatgaactg tgatctgtga
      541 tttgtgaatt caaattttga taataaatta tcaaaattga aaaaatccgc ttgacataat
      601 agataagctt cgatattata atggtagata tatcttcagg gcgaggtgca agtcctcacc
      661 ggcggtaaag cccgcgaacc gaaaggttga tctggttaga ttccagagcc gacagttaaa
      721 gtctggatga aagaagatga tattatcttt agtatttttg ccccgaagat tgcttcgggg
      781 ctttttatat tcccgaagta ataaaaaaca tggagggata tatatgtaca cactcagcaa
      841 ggaagcatgg aacacaagga tgatggtgaa gatatcggta ttgggagtta tttcctttat
      901 attgatgttt ttcgaaatac ctctggtatg gctggcaccg ccgttcatca agatcgacat
      961 ttctgatttg cctgcattga taggtgcatt tgcaatggga cccatggttg gggtattgat
     1021 tcaattgctt aagaacgtac ttaatgttgt aattgaagga actacaactg gagggattgg
     1081 agagctggca aactttgtgg ttggaagtgc atttgcctac acagcaggat tcgtatattt
     1141 caagaaaaag acctttggaa gggctgtagc tggtcttgtg ctgggaacaa ttgtaatgac
     1201 agttgtaatc tcaattgcaa actactacgt aatgttccca ttctatgcaa agcttttcgg
     1261 atggcccatt gagaaccttg tggaaatggg tacggcagta aacagcaata ttgtggatat
     1321 gaaaaccatg ataatctatt ccatcgtacc atttaacctc ttgaagggaa cgattctgac
     1381 agccattacc attttaatat acaagaaggt ttcaccacta ctacacaact agatagaagc
     1441 tgcagacaag gaccttgtgt ccttgttttt tttgaaaaaa atggctttgt ggaaattggc
     1501 aataggctgt gttaagctta gagggtaggt gttaaacaaa tttaatcgag aggtgacaca
     1561 tgattgataa gttaaatgaa gaaatatatg aaatgaaaaa aaggctcaga gagtataaca
     1621 agctgaaatc cttcagagaa tcaatccagg aagagctgac ccacatggta aaagccagag
     1681 gagaccttct taaccagtat gataaggagc tcaaggatgt ggagatgctg gagaaggttg
     1741 gggttaggtc tctattcctg tccatcacag gcaagaagga agagtctctt gatagggaga
     1801 aggaagagct gttaagggca aaggcaagct acgaggaatg ccagaccaat attgaagacc
     1861 tgcagaagga ccttgctgaa gcggataggc tactagccaa atttggagac attgagggta
     1921 acttcaatgg gcttctaaag gaaaaggagg acctcctaat ccagtccggt ggcagtcagg
     1981 gcaaggccct tggagagagg ctgatcaggg ctgaccagct ccaggtggac ataagggaga
     2041 tcagggaggc catcgcagct ggaaggtcca ccctggaatc cctcaagggg gtaagggaga
     2101 aattggattc tgcaaaggga tggggaacct gggaccttct tggaggagga cttatttcaa
     2161 acatcgcaaa gcattcagcc atagatgaag caaacaacag ggccagagag gttcagcacc
     2221 agctgaaggc cttcaggaag gagctttctg atgtaaatga gtttacagac atagaggtta
     2281 acatatcagc ctttgcatcc tttgcagact tcttttttga tggtctcatt gcagactggt
     2341 ttgtccagtc aaagataaat gattccattg gacacgttga ggatgcaaat agaagtattg
     2401 aaggaatagt acaaaatctt cagaataggt tgggtggtct tgaaaaggaa ctccaggaca
     2461 ccaaggaaga aattggaaac attttaagga attaggtttt ttccaacata ttcaccgaaa
     2521 tatttgtgta tggaaaactc atttggtata attgagtatt gacatgattg ggtaatagaa
     2581 ctgagaaatg ggaaggtaaa gcatgaggat agagattaag ggaatagagg aaacagatat
     2641 atttggcaga aggctgggtg agttggtcca gcctggagat gtgatatgct tgaatgggga
     2701 cctgggtgcc ggaaagacca ccctgagcaa gtccgttgga ctgggccttg gagtggagga
     2761 ctacattacg agtccaacct ttacaataat caacgaatac cagggtaggc ttcccctcta
     2821 ccactttgat gtatacagac tcaaggacgg ggaagagctt tatgaccttg gagtggagga
     2881 ttatttctac ggggatgggg tgtgcctgat cgagtgggcc caaaacatcc aggactacct
     2941 gccccaggac aggcttgagc tttggattta cggaacggac aaccccaatg aaagaattgt
     3001 tgaattgaag gcattcgggg atagaagcga gtttctagct aaggagctga ttagatgaaa
     3061 atactgggat tggatacatc cactatgatg acaacatgtg cggtcatgga ggatgaaacc
     3121 ctgctgggag agttttccct aagtcttgac atgagccatt cagaggcact tgtgcccatg
     3181 attggcgagc tgatggacaa tttggggatg aaaatagggg acatggatgg atttgcagta
     3241 tccaagggac ctgggtcctt cacaggactt aggattggcc tggcaacagc caagggcttt
     3301 gcccatgtaa caggtaagcc cattgttggt gtatcctcat tggaggtatt ggccgacaac
     3361 atgtacggtc atgaaattgt tgttccaatg atggatgcca gaagggaccg ggtatttact
     3421 gccatataca ccatggataa tgaccaatgg ctaccagtcc tgaaacctga tgccctgcct
     3481 gttgaagatt tgattcaaat tatcaacagg gaccacgaaa gagtgacact ggttggtgac
     3541 ggagctgtga ggtacaggag tgaatttctt gaagggctca ggggcaaggt cctctttgca
     3601 ccgggaagcc tgaatctcag cagggctgca agcgtctgca ggttgggaat gttaaggctt
     3661 ttaaatggtc agcttgacga catgttcacc atggcacctg agtacctgag ggattcccag
     3721 gctcagagaa tgcagaaaaa gacgaggtaa tcatgaaaat catattgaga gaaatgacag
     3781 agaaggacat agatgaagta cttgaaatag aaagggcctc cttcagtaca ccctggtcca
     3841 gggaatcctt ccagaaggaa gttaccgaga atgcactggc cgtatatatt gtggctgaag
     3901 tggacatgga gcttgtaggc tacggaggac tttggaagat cctggatgaa ggccacatta
     3961 caaatgttgc cgtaaagaag gaatcaaggg gtaagggtat tggagatgca cttgtgatgg
     4021 gtattgtgga ttattgtgac aggacgggga ttccaaatat cacccttgaa gtgagggagt
     4081 ccaatatggt ggccagaaac ctttacaaaa agcatggatt tttggatgca ggcagaaggc
     4141 ctggctatta ttccgacaac aacgaagacg cagtgataat gtggagaaag aagagccaga
     4201 ggtgaaaatg atgttaacat tggcaataga gacatcctgc gacgaaacgt cctgtggggt
     4261 cgtcaaggat ggcagggagg tattatccaa tataatctcc tctcaaatag aaattcacag
     4321 ggaatacggc ggtgtggtgc ctgagattgc atccagaaag cacatcgagg gcatagatgt
     4381 tattgttcag gaggccttgg ataaagcgaa agtgggcttt gaggacatag acttcataag
     4441 cgtgacccag ggacctgggc ttgtgggtgc actgcttgtt ggactttcaa cagccaaggc
     4501 aatggcctat ggactggata ttccaattgt tggtgtaaac cacatagaag gtcatatctg
     4561 tgccaacttt attgagcaca aggacctgga gccaccattt acctgcctga ttgtatctgg
     4621 tgggcactca tacctgattg atgttcttga ttacagcagt tacaggctct atggaaggac
     4681 cagggatgat gctgcaggag aggcgtttga caaggttgca agggctttgg gtctgccata
     4741 tcccggaggt cccttcatag ataggcttgc cttggaggga gacggttctg caatagattt
     4801 cccgagggtc ttcctggagg atgatagtta tgatttcagc ttcagcggcc taaagacagc
     4861 tgttttaaat tatctaaacc agcttaggca aagaaatgag gaaccaattt tagctgatgt
     4921 tgctgccagc ttccagcagg cagttcttga agtaatggtt gaaaaatcat tcaggctggc
     4981 aaaggaaacc ggcagggaca agatagtcat agccggaggg gttgcagcca acaagggctt
     5041 gagagatatg atggagaaga ggggaagaaa ggaaaatgtg gatatctttt acccttcagt
     5101 ccagttatgc acagacaatg ctgccatgat aggttcggca ggctactaca attatatcaa
     5161 tggcagggtc tcagacctcc atatgaaggt aatgccaaat cttgggtttc tagactaaaa
     5221 aaccatcccc aaaatgtcca caagatatgg ggatggtttt tcaactacct gtggataatg
     5281 tgggaaaaaa gaggaaagct actgatatac agtggataac taggtgtaaa cctgtggaca
     5341 atgtggatat gttgtaatga ttactctcca aattccatcc attgtgcata agtagcatct
     5401 aatctgttct ggaggttttc cctttccttg gataattcca ggaccacctc atggtcgtta
     5461 tacatctcgg gatccgaaag tttcttatca atcatttcaa ttttctcctc aagggcagat
     5521 atgttttcct ccaggtttct gatggttctt tttctttccc tttcaaattg aagctggtct
     5581 ttttccttgc gtttttcatg cttaagttgg gttttggtct tctcttcccc ctcctcttcc
     5641 aggtccaatg aaaggaaggc ctttttctcc acatagtaat cgtagttgcc cataaattcg
     5701 gttaccccat cttcggtaag ttcgaatatc ttcgtagcca cctggttcag aaagtacctg
     5761 tcgtgtgaga ttacaaaaat tgtccctgaa tagtttttaa gtgcatcctc aaggacctcc
     5821 ttggagtcta tgtccagatg gttggtaggc tcatccataa gaaggaagtt tgactttgaa
     5881 agcataagct tcaagagggc aacccttgcc ttttccccac cgctgagctc ctttatttcc
     5941 ttgaagatat catcccccac aaagaggaac tggcttaggt aggtccttat ctgatagtgg
     6001 tcaagtaggg ggtattcatc ccaaatctca tccaggaccg tcttatcgag acttaaatcg
     6061 gtcatctcct ggtcaaaata tccaggatat acgtgctggc cgagctttac aattccctgg
     6121 tccaggtcca cctgtccaag aagtatcctg aacagggtgg tcttaccgat tccattggca
     6181 ccaataagtc cgaccttttc ccccctgtat atatcaaggg aaatatccct caacagttcc
     6241 aggtccccga agctcttgca gattccctct ccatggagga cgtcccttcc agaccttgtt
     6301 tccggatcga atttgaaaga agccttcttt gattcagaaa gcttgggcaa aagttttatc
     6361 ctatccaaca ttttctgccg gctacgggcc tgcttgatat acctttcacc cccgtagttc
     6421 atgaatcttt ggatgatctc ctcctggcgc ttgatctcct tctgctggtt gtcatactgc
     6481 cttctcatta tctcaagctc tttcttcctt tgcaccatga agcctgtata gtctgtactg
     6541 tagcttttta gacccctgtt ttccatgtgg aatatcctgt tggccaaatt gtccagaaag
     6601 tacctgtcat gggagatgat gaggcttgaa cccctatagt ccctcaggaa cttttccaac
     6661 cagcctatgg caccta
//
LOCUS       DASRQF010000302         3278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_89584, whole genome shotgun sequence.
ACCESSION   DASRQF010000302 DASRQF010000000
VERSION     DASRQF010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89584"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..1834
                     /locus_tag="VFD79_08115"
     CDS             278..1834
                     /locus_tag="VFD79_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HZH94056.1"
                     /translation="MSILTVKNLNHSFGGREILKDVNFRLLKGEHVGLVGANGEGKSS
                     FMNIITGKLESDEGQIEWAKRVRVGYLDQHAALQKGMTIKDVLKTAFKYLFDLEAEMQ
                     DMYMKMGDVDGDQLSDMLEDVGTIQDILDHNDFYIIDAKIDETSRGLGLTDIGLDKEV
                     NQLSGGQRTKVLLAKLLLEKPDILMLDEPTNYLDEDHIEWLKRYLKEYENAFLLISHD
                     IPFLNSVINLVYHIEDQELSRYVGDYDDFQKVYEAKKAQLESAYKRQQQEIADLEDFI
                     QRNKARVATRNMAMSRQKKLDKIDVIELSKEKPKPKFNFKEGRTAGKEIFKTSELVIG
                     YEEPLTKALNLVMERGQKIALTGANGLGKTTLLKSILGLIPSYSGNVELGQNLEIGYF
                     QQESSSDNNNTCIEEMWKEYPGWTQFEIRAALAQCGLMTKHIESKVMVLSGGEQAKVR
                     LCKIMNRETNILLLDEPTNHLDVDAKEELKRALKAYRGGILLISHEPDFYRDVATDVW
                     NCEGWSTKII"
     gene            complement(1858..2313)
                     /gene="msrB"
                     /locus_tag="VFD79_08120"
     CDS             complement(1858..2313)
                     /gene="msrB"
                     /locus_tag="VFD79_08120"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004817999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HZH94057.1"
                     /translation="MSENIYEKPSEKELKEKLSDISYRVTQENATERPFTSEYDKFSQ
                     DGIYVDIVSGEPLFSSKDKYDAGCGWPSFTKPIEKENIKEKKDLSHMMIRTEVRSKHG
                     DSHLGHVFTDGPKKEGGLRYCINGAALRFIPKDRLEEEGYGEYLNLFKK"
     gene            2605..>3278
                     /locus_tag="VFD79_08125"
     CDS             2605..>3278
                     /locus_tag="VFD79_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH94058.1"
                     /translation="MVLGKGNLLKNKHTNMELEEYKMKLLGKDRLREVIDLQQHVYDN
                     LPNKDVLYMDSYEDMLSDMEEGARVIGVLNSRDRIIAYRYIAFPGRDKRNMGYDIGLN
                     PDELDKVVHLETTVVDPKYRGNDLQSLTLGAAKKMVTQEGYNHFLCTVSPYNFFSLYN
                     IMKNGLKIKALKRKYGSSEDGDDGLWRFILHSDMKKQILNPVDLVISKWANLEMQRDL
                     IEQGYIG"
BASE COUNT          987 a          659 c          750 g          882 t
ORIGIN      
        1 gtttcatctt tccattcctc ctctttccaa tgtaatagac cctttattga taataattcc
       61 atgtttattg tacggcatct tcatggggat gtaaacgtta tccatgaaaa aacagggctt
      121 gcctgcaata atttcagtca acctaactat accctcgcct cactttcagt aaaccttgag
      181 gatagggggg caatcacatg aaattttcac ttaaacaatt ttaattttcc accaatcatg
      241 ttataatcgt cagagtatta attgaaagga gcacacaatg agtattttaa ctgtaaagaa
      301 tctcaatcac agctttggtg gtcgcgagat actaaaggat gttaattttc gactcctgaa
      361 gggagagcac gttggacttg ttggagccaa tggagaggga aagtcgtcct ttatgaatat
      421 aattaccgga aaacttgaat ccgacgaagg tcagatagag tgggcaaaga gggtaagggt
      481 tggttatctg gaccagcatg cggctctaca gaaaggcatg accataaaag atgttctaaa
      541 gacagcattc aagtatcttt tcgacctgga ggcagagatg caggacatgt acatgaaaat
      601 gggcgatgtg gatggggacc agctctctga tatgctggag gatgttggaa ccatccagga
      661 tattcttgac cacaatgact tctatatcat agatgccaaa atcgatgaaa cctcaagggg
      721 gcttggtctc accgacatag gactggacaa ggaggttaac cagctcagtg gtgggcagag
      781 gacaaaggtt ctgctggcca agctactcct ggagaagcct gacatcctaa tgctggatga
      841 gcccaccaac taccttgatg aagaccatat tgaatggctt aagagatatt tgaaggaata
      901 tgagaatgca ttcttgctta tctcccatga tatcccattc ctaaatagtg ttatcaacct
      961 ggtatatcat attgaggacc aggaattgag tcgatatgtt ggagactacg atgacttcca
     1021 aaaggtatac gaggccaaga aggcacagct ggagtcggct tacaagagac agcagcagga
     1081 aatagcagac ttggaggatt ttatccaaag gaacaaggca agggttgcca caaggaacat
     1141 ggcaatgtcc aggcagaaaa agctggataa gattgatgtg attgagcttt caaaagagaa
     1201 gccaaagcct aagttcaatt tcaaggaggg caggactgca ggaaaggaaa tcttcaagac
     1261 cagtgagctt gtgatcggat acgaggagcc acttacaaag gcacttaatc ttgtcatgga
     1321 gaggggacag aagatagcac ttactggagc caatggcctt ggcaagacca ccctgttaaa
     1381 gtctattctt ggacttatcc cttcctattc aggcaatgtt gagcttggac agaatctgga
     1441 gataggatac ttccagcagg aatcttcaag cgataacaac aatacctgca ttgaggaaat
     1501 gtggaaggaa taccctggct ggacccagtt tgaaatcagg gcggcactgg cccagtgcgg
     1561 gcttatgacc aagcacatag aatccaaggt catggtcctt agcggcggtg aacaggccaa
     1621 agtaagactt tgcaagatca tgaacaggga aacaaacata ctccttcttg atgagcccac
     1681 aaaccacctt gatgtggatg ccaaggagga gcttaagaga gctcttaagg cctacagagg
     1741 tggaatcctc ctgatttccc atgaacctga cttttatagg gacgtggcga ctgatgtatg
     1801 gaactgtgaa ggctggagta caaaaataat ctaaagggag gcttgcctcc ctttagatta
     1861 ttttttgaac aaattcaggt attcaccgta accttcctct tccagcctgt cctttgggat
     1921 aaaccttagg gcggcaccat ttatgcaata tcttagccct ccctccttct ttggtccatc
     1981 cgtgaagaca tgacccagat gggaatctcc atgtttactc cttacctctg ttcttatcat
     2041 catgtggcta agatcctttt tctcctttat attctccttt tcaatgggct ttgtaaagct
     2101 gggccagccg cagcctgcat cgtatttgtc ctttgacgaa aagagcggtt ctccggatac
     2161 tatatccaca taaataccgt cttgcgagaa tttgtcgtac tcacttgtaa atggtctttc
     2221 agtggcattt tcctgtgtga ccctgtagga aatatcacta agtttctcct tcagttcttt
     2281 ttctgatggc ttttcatata tattttcaga cacctatatg cacctcctga gtaattttat
     2341 accccaaaaa atcattatta tatattctga atattccgtg attagcaaat tcgagtgatt
     2401 ccctgcaaat gttttcatgt ctgagtttac acgtttcctt gcgtactgca tccatttttt
     2461 ctctttgcag cctcttcaaa tgcacgttta gggacaaatt atttttgttg acatatacta
     2521 aattcatagt atataataaa cttacaactt gatgacaggt tgcaggttgt cggttgagac
     2581 gttaaaggaa gggaggacat atatatggtg cttggaaagg gcaatttatt aaaaaataaa
     2641 cacaccaaca tggagcttga agagtataaa atgaaattac tgggaaaaga taggctaaga
     2701 gaggtcattg atcttcagca acatgtatat gataatcttc caaacaagga tgttctctac
     2761 atggattcat acgaggacat gctttcggat atggaggaag gcgcgagggt aatcggtgta
     2821 ctgaacagca gagaccgcat aatcgcatac agatatatag cattccctgg aagggataag
     2881 agaaatatgg gttatgatat cggactgaac ccggatgaac tggacaaggt tgtacacctt
     2941 gagaccacgg tcgtggatcc aaaatacaga ggcaatgacc tgcaaagtct cactcttgga
     3001 gcagccaaga agatggttac ccaggaaggc tacaatcatt tcctgtgtac agtatccccg
     3061 tataatttct tcagccttta caacataatg aaaaacggcc tgaaaataaa ggccctgaag
     3121 agaaaatatg gatcctcaga ggatggggat gatggactgt ggagattcat ccttcatagc
     3181 gacatgaaga aacagattct taatccagtg gaccttgtga tttccaaatg ggctaacctt
     3241 gagatgcaaa gagacctgat cgagcagggg tacattgg
//
LOCUS       DASRQF010000303         4592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1294722, whole genome shotgun sequence.
ACCESSION   DASRQF010000303 DASRQF010000000
VERSION     DASRQF010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294722"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..1206
                     /locus_tag="VFD79_08130"
     CDS             244..1206
                     /locus_tag="VFD79_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HZH94059.1"
                     /translation="MRKILITGGLGQIGSELTKKMRDIYGKDNVVMSDMQAKDSHKEL
                     IESGPFQVLDVLDREKMGEIVDRYKIDTIVNLAAILSAVGEAKPQLCYNVNMNGLYNV
                     LELAREKDLSVFTPSSIAVFGESTPKDKTPQDTIMRPGTMYGVTKVAGENLCDYYYHK
                     FGVDTRGVRFPGLISNDTLPGGGTTDYAVHIYYEALKEKKYTSFIDKGTKMDMMYMPD
                     ALNAIVQLLEADPSRLKHRNAFNITAMSFDPEDMAASIRKHIPEFKLDYDVDPVRQAI
                     ADSWPNSLDDSEARAQWDWNPEYLLDDMTADMLNSLRKKLNIQA"
     gene            1332..1769
                     /locus_tag="VFD79_08135"
     CDS             1332..1769
                     /locus_tag="VFD79_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HZH94060.1"
                     /translation="MKLSTKGRYGLMAMYQLAQDHGQGPISLKYVAEKQGLSENYLEQ
                     LFSSLKKDGLLESVRGAYGGYMLSRKPEEITVGEVLRSLEGQMSPSDCVADGIHDCSR
                     DDKCATQWVLGQIKNSIDQVIDSITLMDMIRDDKGLNTELEGD"
     gene            1769..2947
                     /gene="nifS"
                     /locus_tag="VFD79_08140"
     CDS             1769..2947
                     /gene="nifS"
                     /locus_tag="VFD79_08140"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HZH94061.1"
                     /translation="MDKMIYMDHSATTPVKKEVLQEMLPYFSEYYGNPSSVYQLSSKS
                     KLAIDKARDQVAKGLGAKKSEIYFTGGGSEADNWAIKGIAYRHRDKGNHIITTKIEHH
                     AVLHTCEYLENQGFEVTYLDVDEYGMISLEDLEGAIRDNTILISIMFANNEIGTVQPV
                     KEIGEIARRHKVLFHTDAVQAVGSIKIDVNEMNIDLLSMSAHKLYGPKGVGALYIRQG
                     TRIDPLIAGGAQERNKRAGTENIAGIVGTGKAVELAYEHLEENNRKLISLRERLIKGI
                     NGKIPHVRLNGHPEKRLPGNVNFCFQFIEGEALLLSLDMVGVAGSSGSACTSGSLDPS
                     HVLLSIGLPHEIAHGSLRLSLGLGNTEEEVDYVVDQLVGIVDRLRKMSPLYDKVQGGI
                     "
     gene            2948..3388
                     /gene="nifU"
                     /locus_tag="VFD79_08145"
     CDS             2948..3388
                     /gene="nifU"
                     /locus_tag="VFD79_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HZH94062.1"
                     /translation="MYSEKVMEHFTQPRNVGEIENADGVGEVGNAKCGDIMRMYLKID
                     GDVITDVKFKTFGCGSAIASSSMATELIKGKTVKEAMELTNKAVADALDGLPPVKMHC
                     SVLAEQAIKSALVDYSKKSGVKIEGLEDVDFDDEHLHDLPEDDE"
     gene            3471..>4592
                     /locus_tag="VFD79_08150"
     CDS             3471..>4592
                     /locus_tag="VFD79_08150"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZH94063.1"
                     /translation="MSINTPPSLFVNLVWILSALLLALIIYYLINIGNRFVPGKKVIR
                     YNTRLILWVIITLFGIYFVTLVFRRYTLVADTFFTIIISMILAYFLNPLVNFFERKGL
                     KRFLATIAVYLLILGVISILTVSVLPRTGQEIRRLATNFPGYVNTVSLWMTNLYAGYS
                     DTLVEMPELLKSIEDVISQNLGRIQTGIANGVERFILSIIGLFTRIISWILTPILTFY
                     FLVDKEHFIDKFRRMIPKKHKEETLDLLREIDTALSQFIRGRLLMAVMVGIATTIFLF
                     LMGVEFAIVIGFITGLADIIPYIGPFLGFVPAVIFAFIASPIKALWVGIFFILIQWAE
                     NNILGPKVLGKTTGMHPLTILFAIIVGGTIFGVMGMILSV"
BASE COUNT         1403 a          929 c         1139 g         1121 t
ORIGIN      
        1 aggaaagata tacaatattg tcgtaaaata acttgcaaaa atgattctgt tgattttcag
       61 cagaatcatt tttttttaca cctggttaca ggcagttggc tgtttcaaga gaggagagcc
      121 taagtacgtg aagattctat acaattttaa tattctggat aattcaattg ctgtatgaat
      181 tttttccgag tatgttataa tgtagacgaa gttatttaaa atcaacggag gtgcattaaa
      241 tatatgagga agatccttat aaccggcggc cttggacaaa taggttcaga gctgaccaag
      301 aagatgaggg acatctacgg caaggataat gtcgtaatga gtgatatgca ggccaaggac
      361 agccataagg aactgataga gagcggaccc ttccaggtgc ttgatgttct ggacagggaa
      421 aagatgggag agattgtgga caggtacaag atcgacacca tagttaacct ggctgcaatc
      481 ctgtcagcag ttggtgaagc caagccacag ctatgctata acgttaacat gaacggactc
      541 tacaacgtac ttgagcttgc aagggagaag gacctgtcgg ttttcacacc aagctcaata
      601 gcggttttcg gtgagagtac cccaaaggac aagacaccac aggatacaat aatgagacct
      661 ggaaccatgt atggggtcac caaggttgcg ggagaaaatc tttgcgacta ctactaccat
      721 aaatttggag tggatacaag aggagtaagg ttccctggac tgatctcaaa cgacaccctt
      781 ccaggtggag gaaccacaga ctacgcagtc cacatctact acgaggcatt gaaagagaag
      841 aaatacacaa gctttataga caagggaact aagatggaca tgatgtacat gccggatgcc
      901 ctgaacgcaa ttgttcagct tcttgaggca gacccatcaa gacttaagca tagaaatgcc
      961 ttcaatatca ctgcgatgag ttttgatccg gaggatatgg ccgcttccat caggaagcac
     1021 atacctgagt tcaagctgga ttacgatgtt gatccagtaa gacaggcaat tgctgattca
     1081 tggcctaact cactggatga ttccgaagcc agggcacagt gggattggaa tccggaatac
     1141 ctgcttgatg acatgacggc agacatgctg aacagtttaa ggaagaaact aaatatacag
     1201 gcctaacagg caaaacaagg ctgggaattt tcccggcctt gtgtgctttt caatgttgac
     1261 aagattaccc ggatatacta tactatatta ggaataccaa ctaaataagt tgggattaaa
     1321 gaggtgatca aatgaaattg tcaaccaagg gaagatatgg cctaatggcc atgtaccaac
     1381 tggcccagga ccatgggcag ggaccgattt ccctgaaata tgtggctgaa aaacagggtt
     1441 tgagtgaaaa ctacctggaa cagctttttt caagtctaaa gaaggatgga ctccttgaaa
     1501 gtgtaagggg agcctacgga ggctacatgc tttcgagaaa gcctgaggaa ataactgttg
     1561 gagaggtcct gagatctctt gaaggacaga tgtccccctc tgattgcgtt gcagacggaa
     1621 tccacgattg cagcagggat gacaaatgtg caacccagtg ggttcttggc cagatcaaga
     1681 acagcatcga ccaggttatt gactccataa cccttatgga tatgattagg gacgacaaag
     1741 gattaaacac tgaattggag ggagattaat ggataagatg atatacatgg accactcggc
     1801 tacaacacct gtaaagaagg aagtcctcca ggagatgctt ccatattttt cagagtacta
     1861 cggcaatcct tcaagcgtct atcaattgag cagcaagtca aagcttgcca ttgacaaggc
     1921 aagggaccag gttgcaaagg gactgggtgc gaagaagagt gagatttatt tcacaggcgg
     1981 tggatctgaa gcggacaact gggccataaa gggaatagcc tacagacacc gtgataaggg
     2041 aaaccacatc atcacaacaa agattgaaca ccacgctgta ctccacacat gtgagtacct
     2101 tgaaaaccaa ggttttgaag tgacctatct cgatgtggac gaatatggaa tgatcagcct
     2161 tgaagacctg gaaggggcaa tcagggacaa taccatactt atatcaataa tgtttgcaaa
     2221 caacgagatt ggaactgttc aacccgtcaa ggaaattgga gagattgcaa gaagacacaa
     2281 ggtacttttc cacacggatg cagttcaggc agtgggcagc ataaagatag atgttaatga
     2341 aatgaacata gaccttcttt caatgtctgc acacaagctg tacggaccaa agggagtggg
     2401 agcactttac atcagacagg gaacaaggat tgacccacta atcgctggag gcgcccagga
     2461 aaggaacaag agggcaggaa ctgagaacat tgcgggaatc gtagggacag gcaaggccgt
     2521 cgagctggcc tatgagcacc ttgaggaaaa caacaggaaa cttatctcct tgcgggagag
     2581 gctgatcaag ggcataaacg gaaagatccc ccatgtgaga ctcaatgggc atcctgaaaa
     2641 gagactgccg ggaaatgtga acttctgctt ccagtttatc gagggagaag cactccttct
     2701 tagccttgac atggtagggg tggcaggatc aagcggatct gcatgtacat caggatcact
     2761 tgacccgtca cacgtactgc tttcaatagg ccttccccat gaaattgccc acggatccct
     2821 gagactgtct ctgggtctag gcaataccga ggaagaggtg gactatgtag tggaccagct
     2881 tgtgggaata gttgacaggc taagaaagat gtcaccgtta tatgataaag ttcagggggg
     2941 aatataaatg tattcagaaa aggtaatgga gcacttcact cagccacgta atgttggtga
     3001 aattgaaaat gcagatgggg taggtgaagt aggcaatgca aaatgcgggg acattatgag
     3061 aatgtacctt aagatagatg gggatgtaat aaccgatgta aagttcaaga cctttggctg
     3121 tgggtcagca atagcatcat caagcatggc tacagagctt ataaagggaa agacggtcaa
     3181 ggaagccatg gaattgacca acaaggccgt agcggatgcc cttgacggcc tgccaccggt
     3241 aaagatgcat tgcagcgttt tggctgaaca ggccataaaa tcagcacttg ttgattattc
     3301 aaagaaatcg ggggtaaaaa ttgagggact ggaagatgtt gactttgatg atgagcatct
     3361 acacgacctt cctgaggatg acgaataata aatattgacc tatagagact ttggttagag
     3421 atggcgcaga aacatccagc catggtctct gtttgttcag gggtgatcag atgtcaataa
     3481 atacgccacc ttctctcttt gttaatctgg tatggatcct gtcggcccta ttgctggccc
     3541 taataatcta ctacctgatc aacatcggaa acaggtttgt accggggaaa aaagtcataa
     3601 gatacaatac caggctgata ctgtgggtaa taataaccct tttcggcatc tattttgtta
     3661 cgcttgtttt cagaaggtac acgctggtgg cagacacctt cttcaccata atcatatcaa
     3721 tgatcctggc atattttctg aatccccttg ttaatttctt tgaaagaaaa ggactcaaaa
     3781 gatttttggc caccatagcc gtctatctcc ttattcttgg agtcatctcc atacttacag
     3841 tatccgtcct gccaaggaca ggtcaggaaa tcaggaggct ggccaccaat tttccaggct
     3901 atgtgaacac ggtatccctg tggatgacca atctttatgc gggctattca gataccctgg
     3961 ttgaaatgcc agagcttctc aagagcatag aggatgtaat atcccagaac cttggaagga
     4021 ttcaaacagg aatagccaac ggtgtggaga ggtttatcct tagcataata ggcttgttca
     4081 caaggataat aagctggatc ctgactccaa tactgacctt ctacttcctg gttgacaagg
     4141 aacacttcat cgacaagttc agaaggatga ttcccaaaaa gcacaaggaa gagacccttg
     4201 acctcttgag ggaaatcgac actgccttgt cccagttcat aaggggcagg ctactgatgg
     4261 ctgtcatggt tggaattgcc acaactattt tccttttcct tatgggagtg gaatttgcca
     4321 ttgtaatagg attcataaca ggacttgcgg atataattcc atatatcgga ccattcctgg
     4381 gatttgtccc tgctgtcata tttgccttta tcgcaagccc catcaaggcc ctttgggtgg
     4441 gaatcttctt tatccttatc cagtgggcgg aaaacaacat acttgggccc aaggtattgg
     4501 gcaagacaac gggaatgcat ccacttacga tcctctttgc aatcatagtt ggaggtacca
     4561 tcttcggtgt tatggggatg attctttctg tt
//
LOCUS       DASRQF010000304         4157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1063828, whole genome shotgun sequence.
ACCESSION   DASRQF010000304 DASRQF010000000
VERSION     DASRQF010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063828"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1355)
                     /locus_tag="VFD79_08155"
     CDS             complement(<1..1355)
                     /locus_tag="VFD79_08155"
                     /inference="COORDINATES: protein motif:HMM:NF012563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HZH94064.1"
                     /translation="MTILDQRPDEEIQKILYQHGAVTLEEASSKEIYRALSYHIRETI
                     GRQMSRTEKNSRQGMTMVYMSMEYLPGSLLRKNIDYLHKGKSLQEALQKRGCNLNDLI
                     LEDRELGLGYSDIGYLSNGLMDTFASGSYNAIGYGLLYREGYFRQEVENFQQLEKPDN
                     WWVRGKNWLYKGDRKYRVTTGGRVDISMGDKGLVFTPSDTEDLTLRYYDLPYLGYRNG
                     FCNRLRLFDHETITRRIKPLDSTTENRRERFKQEYLLVSGALQDVIKTHLSNGRKVET
                     LNDHFIFLMMDTNLLLAVPELMRLLVDQYKLEWDHAWEITSEAFFYTPLVALEDTLLT
                     LEGKTFREWLPRLWMVLEEIHHRYLDRLRSIGEILDEGIKSSGILWDDQVRLYNLPGA
                     GVYEGPPIGHPLAVSHRRWILSGNPPLRDLIEAYLGDEFVENPEVIKRLLKMEDDRDL
                     LL"
     gene            complement(1357..2793)
                     /gene="glgA"
                     /locus_tag="VFD79_08160"
     CDS             complement(1357..2793)
                     /gene="glgA"
                     /locus_tag="VFD79_08160"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004836053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HZH94065.1"
                     /translation="MKILFVASEMAPFAKTGGLADVAGSLPYALQEEGHQVSVVLPLY
                     SKVGDKYRQKMKKIRDFYVDLDWRRQYAGIHLYQEDGLDVYFVDNEYYFHRHALYGMY
                     DDGERFVFFNKAVVQMIKELDLETDIVHANDWHTGLIPIYIKDFGKGDQYYRDIKTVF
                     TIHNLKYQGIFPPSILGLAGISRDYYHEEGLKYYEMVNFMKAGIVYCDALTTVSQSYA
                     QEIKYEFYGESLQGIIRRHEYKLMGIINGIDYKLYNPETDKRIPYHYSKEDPGDKARN
                     KEALQEAFGLPVKADVPVLSMVSRLVDMKGLDLVRYILDELLQDDIQLVVLGTGEKEY
                     EDMFRHFQWKYPDKMSAHIYFNEEEAHLVYAGSDIFLMPSMAEPCGISQLIALRYGTV
                     PVVREAGGLKDTVIPYNGFTQEGTGFSFANYNAHELLHTIRRALEVYSDKDSWDGLVK
                     RTMEAKHDWEKSSKEYTKLYNTLLHQRW"
     gene            complement(2799..3923)
                     /gene="glgD"
                     /locus_tag="VFD79_08165"
     CDS             complement(2799..3923)
                     /gene="glgD"
                     /locus_tag="VFD79_08165"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR02092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HZH94066.1"
                     /translation="MEKCLGIISGANIENNFGALCVHRPVYMLPFAGRYRLIDFSLSN
                     MVNHGIRTVAVYTGEKVRSTMDHVGDGKAWDLNRRFNGLFLFPPVSHGLSAKRTGEIP
                     QYYTTKDFFDQAKENYVFINHPNILAKVDLNEAFRFFRESDADITFIYKKQSDPFGEY
                     VNTQKMNIGDDGTVLNIGTNLGTEREFNMYLKMGFLKKEVFMKLVKEAIERGESDNLL
                     ETILINKDKYKLNSYEFKGHVESIRNLKSFYDANMNLLKKDIARELFYSNGIIYTKTK
                     DEPSTLYTEDSSVHNSLVANGCVIEGTVENSIIFRGVKIKKGAIVKNSIIMQKSEIHE
                     NAAVINSIMDKQGIVGEDVRIAGSMLMPFIVEKKQEIRKD"
     gene            complement(3937..>4157)
                     /gene="glgC"
                     /locus_tag="VFD79_08170"
     CDS             complement(3937..>4157)
                     /gene="glgC"
                     /locus_tag="VFD79_08170"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of ADP-glucose and
                     diphosphate from ATP and alpha-D-glucose 1-phosphate;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HZH94067.1"
                     /translation="TLMNSILFSDVVIEDDAVVVDSVVHSNCVIEKGAKVYRAVISEG
                     MTVKAGEVIGQEDSENIYLVSEEGISVE"
BASE COUNT          922 a         1095 c          881 g         1259 t
ORIGIN      
        1 agaagcaggt ccctgtcatc ttccatttta aggagccttt ttataacttc cggattctcg
       61 acaaattcat cccccaagta agcctctatc aggtccctta agggaggatt ccctgaaagt
      121 atccaccttc tgtgggatac agccagggga tgtccaattg gtggcccctc atacactcca
      181 gccccaggta ggttgtatag cctgacctgg tcatcccaaa gaattcctga ggattttatc
      241 ccctcatcca agatttctcc gatgctcctt agcctgtcca ggtacctgtg gtggatttcc
      301 tcaaggacca tccagagtct tggaagccat tccctgaagg tctttccttc aagggtgagg
      361 agggtgtcct caagggctac caggggggtg tagaagaagg cttcagatgt tatctcccag
      421 gcatggtccc actccagctt gtactggtca acaagcagcc tcataagctc tggaactgcc
      481 aaaagcaggt ttgtatccat catgaggaat atgaagtggt catttagggt ctccaccttt
      541 cttccattgg aaaggtgggt ctttatgaca tcctgaagag ctccagaaac caggaggtat
      601 tcctgcttga acctttccct cctgttttcc gtcgtggaat caaggggctt tatccttctg
      661 gttatggttt cgtggtcaaa tagccttagg cgattgcaaa agccatttct gtagcccagg
      721 tagggcaggt cgtaatacct tagggtaagg tcctctgtgt ctgaaggggt gaagacaagc
      781 cccttgtctc ccatggatat gtccaccctg ccaccggttg tcaccctgta tttcctgtct
      841 cccttgtaga gccagttctt gcccctgacc caccagttgt ccggcttttc cagctgctgg
      901 aaattctcca cttcctgacg gaaatagcct tccctgtaga gaaggccata gccgattgcg
      961 ttgtatgaac cacttgcaaa tgtatccata agtccgtttg aaaggtatcc gatgtcagaa
     1021 tatccaaggc ccagctccct gtcctccaaa ataaggtcat tgaggttgca ccctctcttt
     1081 tggagggctt cctggagtga tttgccctta tgaagataat ctatgttctt tctcaggagg
     1141 cttcccggaa ggtactccat ggacatgtag accatggtca tcccctgcct gctgtttttc
     1201 tcagtccggg acatctgcct tcctattgtc tccctgatat ggtagctaag ggccctgtag
     1261 atctccttgg aggatgcctc ctccagggtc acagctccat gctggtagag tatcttttga
     1321 atttcttcat ccggtctttg gtctaaaata gtcatattac cacctctggt gcagcaatgt
     1381 attgtagagt tttgtgtatt cctttgagga tttctcccag tcgtgctttg cctccattgt
     1441 ccttttcaca aggccatccc aggaatcctt gtctgaatat acctcaaggg ccctccttat
     1501 tgtgtggaga agctcatggg cattgtagtt tgcaaaggaa aatcctgtcc cctcctgggt
     1561 gaacccgttg tatggaataa ctgtatcctt aagtcctcca gcttccctta caacaggtac
     1621 ggttccatac cttagtgcaa taagctggga tattccacag ggctctgcca ttgaaggcat
     1681 taggaaaatg tccgaacctg catataccag gtgggcctcc tcctcgttga agtagatgtg
     1741 ggctgacatc ttgtccggat acttccactg gaagtgtctg aacatatcct cgtattcctt
     1801 ttcgcccgtt ccaaggacca caagctgtat atcatcctga agaagctcat ccaggatgta
     1861 tctcaccagg tccagcccct tcatgtccac aagtcttgac accattgaaa ggactggaac
     1921 atcagccttt acaggcaatc caaaagcctc ttgcagggcc tccttgttcc tggccttgtc
     1981 acctggatcc tcctttgaat agtggtatgg tatccttttg tccgtttccg gattgtaaag
     2041 cttgtaatct attccgttta ttattcccat tagcttgtac tcgtgccttc tgattatccc
     2101 ctgaagactt tccccgtaaa actcgtactt tatctcctgt gcataggatt gggacacggt
     2161 tgtcagggca tcacaataga cgattccagc cttcatgaag ttcaccatct cgtagtactt
     2221 gagaccctcc tcatggtagt agtctctcga tatccccgca agacccagta ttgaaggagg
     2281 gaatatcccc tggtacttga ggttgtgtat tgtaaagacg gtcttgatgt ccctgtaata
     2341 ctggtctccc tttccaaaat ccttgatgta tatgggtatc agtcccgtgt gccagtcatt
     2401 tgcatgaact atgtcggttt ccaggtccag ctccttgatc atctggacta cagccttgtt
     2461 gaaaaacaca aacctttccc catcgtcata catgccgtag agggcatgcc tgtggaagta
     2521 gtactcgttg tcaacgaaat acacatccag gccatcctcc tggtagagat ggattcctgc
     2581 gtactggcgc ctccagtcaa ggtccacata aaagtcccta atctttttca ttttctgtct
     2641 gtacttatct cccaccttgg aatagagggg aagaacaaca gacacctggt gtccctcctc
     2701 ctgaagggcg tagggcagtg agcccgccac atcagccaag cctccggtct ttgcgaaggg
     2761 ggccatttca gaggccacaa ataatatttt catccccatc aatcctttct gatttcctgc
     2821 ttcttctcca ctataaacgg catcagcatg ctgccggcta tccttacgtc ctctccaact
     2881 attccctgct tgtccataat ggagttgatg accgctgcat tttcatgaat ttcagacttc
     2941 tgcatgatta ttgaattctt gactattgcg cccttcttga tcttgactcc cctgaatatt
     3001 attgaattct caaccgttcc ttcaattacg catccattgg cgacaagtga attatggacc
     3061 gatgagtcct cagtgtatag ggttgatggt tcgtcctttg tttttgtgta tattattcca
     3121 tttgaataga agagttccct ggctatatcc tttttcaaga ggttcatgtt tgcatcgtag
     3181 aagcttttga ggttcctgat gctctcaacg tgtcccttga actcgtagct gttgagcttg
     3241 tatttgtcct tgttgatcag gatggtctca agaaggttat ccgactcccc tctctcgatg
     3301 gcctccttca caagcttcat gaaaacctcc ttcttaagga atcccatctt caggtacatg
     3361 ttgaattccc tttcagtacc caggtttgtc cctatgttaa gaacggttcc gtcatcacct
     3421 atattcatct tttgggtatt gacatactct ccaaaggggt cactctgctt cttgtagata
     3481 aaggtgatat ccgcatcact ctccctgaaa aacctgaagg cctcatttag gtccaccttg
     3541 gcaagtatat tggggtggtt tatgaataca taattttcct tagcctggtc gaagaagtct
     3601 tttgtggtgt aatactgggg aatctccccc gtcctttttg ccgacaggcc gtgactgacc
     3661 ggtggaaaca ggaagagtcc gttgaacctt ctattcaggt cccatgcctt tccatcaccc
     3721 acatggtcca ttgttgacct gaccttctcc cctgtgtaaa ctgcaactgt ccgtatcccg
     3781 tgattgacca tgtttgacag actgaagtcg ataagcctgt acctccctgc aaaaggaagc
     3841 atatataccg gtctgtggac acaaagggct ccaaagttgt tttctatgtt tgcaccgcta
     3901 attattccta gacatttttc catccttttc gcctccttat tccaccgaga ttccttcctc
     3961 agagacaagg tatatgttct ctgagtcttc ctgtccgatc acttctcctg cctttacggt
     4021 catcccttct gatatgactg ccctgtatac cttggctccc ttctcgatga cgcagttgga
     4081 atggaccacg ctgtcaacca caacagcatc atcctctatg accacatcac tgaagagtat
     4141 gctgttcatc aaggtac
//
LOCUS       DASRQF010000305         3785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1325192, whole genome shotgun sequence.
ACCESSION   DASRQF010000305 DASRQF010000000
VERSION     DASRQF010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325192"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /gene="mazG"
                     /locus_tag="VFD79_08175"
     CDS             <1..1135
                     /gene="mazG"
                     /locus_tag="VFD79_08175"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HZH94068.1"
                     /translation="GMSFIEPTLEVAKADPVDGLKILDGIGLDGFQVDINSHQIFTQV
                     YNKRVMSEVKLVLMDIYPEDYGVILVHKGGMGESEIVERIPLRQIDRSEHIGSLTSLF
                     VPRINKSQHGIHDFSDIQRIMKRLRDEDGCPWDREQDHLSIRGAVLEEAYELVEALQD
                     DDPDHIVEELGDLLLQVVFHAQIGHENGDFNPIQVTSTLANKLITRHPHVFFEKNVDN
                     SEEVVYNWNKIKYANRNITTLSEKLRNIPHLPALLRSYKVQEKAADVGFDWDVIEGPS
                     DKVGEELAEVLEAASTHGPGSLVVESEVGDLLFAAVNLSRFLEVNPEVALGRTIEKFT
                     KRLEEMEYMADRMGIVMEEMTLEELDSLWDKVKLRENGETRTD"
     gene            1179..1475
                     /locus_tag="VFD79_08180"
     CDS             1179..1475
                     /locus_tag="VFD79_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HZH94069.1"
                     /translation="MNKAELIASIAEKSNLTKKDAEAALNAFMGSVEESLAAGNKVQL
                     VGFGTFEVRERKAREGRNPRNPEEVIKIPASKAPVFKAGKALKESVNVKKGKKK"
     gene            1534..1776
                     /locus_tag="VFD79_08185"
     CDS             1534..1776
                     /locus_tag="VFD79_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HZH94070.1"
                     /translation="MRLDKYLKTARIIKRRTVAKEACDQGKVTINGKVAKAGDNVSVG
                     DRITLSFGSGQMNIEVVQVKDNAKKDEAHELYKSLD"
     gene            1855..2187
                     /locus_tag="VFD79_08190"
     CDS             1855..2187
                     /locus_tag="VFD79_08190"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HZH94071.1"
                     /translation="MSDKRTNKRKGIRIFHVLLILLVVYVGFVMNHQRRLMNNLQDRR
                     TAVQMQISSLEREIKILNDEIEKSGTLEFIEKVARDDLGMVKPREIIYVDKSLEGNNT
                     LEIFKNDN"
     gene            2267..2683
                     /locus_tag="VFD79_08195"
     CDS             2267..2683
                     /locus_tag="VFD79_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HZH94072.1"
                     /translation="MSVSVGEIVEGTVTGITNFGAFVQLPEGKSGLVHISEVSHDYVE
                     KVSDYLKKDQKIKVKVLSISADGKISLSVRQAKPKTSKPVEIDWAKSDDRQKNMSFED
                     MLNSYLKDSSEKMDQIKTKDGRKSYGMKGKKASNND"
     gene            2785..>3785
                     /gene="tilS"
                     /locus_tag="VFD79_08200"
     CDS             2785..>3785
                     /gene="tilS"
                     /locus_tag="VFD79_08200"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HZH94073.1"
                     /translation="MIDLVRSNITRHKLLENIDTVLAAVSGGPDSMAMLYCLYELRDQ
                     FEFKLLVAHVNHGVRGELARRDQNFVEKISKDLGLDYYTTNVDMAAYGKAHGMTSEEA
                     GRLLRYGFFRDVLEENGGGRIAVAHNKNDQAETVLHRIIRGTGLDGIRAMSMISGDII
                     RPLLNISRDDIESFIEDRGIASVHDHTNLQAVYTRNKIRLELLPYLSDNFNPNIVDSL
                     YRLSEIAQLDLEVLDREIEKKYNLLVKKRTNNSIIFKGEQFIKEDEGSARRLVRRAIL
                     DVVGDLNGFGEVHIQSAANIFLTGDTGKSVHLGRNVMAEVSYRDFAIKVGGTESLKF"
BASE COUNT         1268 a          617 c          981 g          919 t
ORIGIN      
        1 aggtatgagt ttcatagaac caacactgga ggttgccaag gcagacccgg tggatggatt
       61 gaagattctg gatgggattg gcctggatgg attccaggtg gacataaact cccaccagat
      121 atttacccag gtgtataaca agagggtcat gtctgaggta aaactggtcc taatggatat
      181 ttaccctgag gattacgggg tgatcctggt tcacaagggt ggaatgggag aaagtgaaat
      241 tgtcgagagg attccccttc gtcaaattga cagaagtgag cacattgggt ccctgacaag
      301 tctttttgtg ccccgcatca acaagtccca gcacggaatc catgatttca gcgacataca
      361 aaggataatg aaaagactga gggatgagga tggatgtccc tgggacaggg aacaggacca
      421 cctaagcata agaggggcag ttcttgagga ggcctatgaa ctggttgaag ccctccagga
      481 tgacgatcct gaccacatag tggaggagtt gggagattta ttgcttcagg tggtatttca
      541 tgctcaaatc ggccatgaaa atggtgattt caacccgatt caggtgacaa gtacccttgc
      601 aaacaagtta attactaggc atccacacgt tttttttgaa aaaaatgtgg ataattccga
      661 agaagtagta tataattgga ataagataaa gtatgcaaac aggaatatta ctacactttc
      721 agagaaattg agaaatattc cacatctgcc ggcacttctt aggagctaca aggttcagga
      781 gaaggcagcg gatgttggat ttgactggga cgtgatagaa ggcccatctg acaaggtggg
      841 agaggagctg gctgaagtgc ttgaagccgc ttcaacacac ggtccaggca gtcttgttgt
      901 tgaaagtgaa gtgggcgatt tgttgtttgc tgctgtgaac ctatccagat ttctagaagt
      961 caatcccgag gttgcattgg gtagaaccat tgaaaaattc acaaaaagat tagaggaaat
     1021 ggagtacatg gctgatagaa tgggtatagt tatggaagaa atgaccttgg aggaattaga
     1081 tagcctctgg gataaggtca agctccgtga aaatggggag accaggactg attaagttta
     1141 gtatttctag aaaataaaaa aacaaggagg aattattaat gaacaaagca gaattaattg
     1201 caagcattgc ggagaagagt aatcttacca agaaggatgc agaggcagct ttgaatgcat
     1261 tcatgggatc agtagaggaa tcattggcag caggaaacaa ggtacaattg gtaggattcg
     1321 gaactttcga ggttagagaa agaaaagcca gagaaggtag aaacccaaga aaccctgaag
     1381 aagtcattaa aattccagca tcaaaggcac ctgtattcaa ggcaggaaaa gcgcttaagg
     1441 aaagcgtaaa cgtaaaaaaa ggtaagaaga aataatacaa acaggaaatc aggtctttga
     1501 cctgattttt tttgtgggaa ggagaggact taaatgagat tggacaagta tctcaagacg
     1561 gcaaggataa taaagagaag aaccgtagcc aaggaagcct gtgaccaggg aaaggttaca
     1621 ataaacggca aggtggccaa ggcaggggat aatgtcagtg ttggggacag gataaccctt
     1681 agcttcggaa gcggtcagat gaacattgag gtggttcagg taaaggacaa tgcgaaaaaa
     1741 gatgaagctc atgagctcta caaatccctg gactgataca aaagtagctt gaaaatattt
     1801 ctaaatagtg acataataaa ggagtatgga ggcatttgaa aaggtggtgt tgatatgtct
     1861 gacaagagaa cgaataaaag aaaagggatt aggattttcc atgtcctttt gattcttctt
     1921 gttgtttatg ttggatttgt tatgaatcac cagagaaggc tgatgaacaa ccttcaggac
     1981 cgcaggacag ctgtccagat gcagatcagc agccttgaga gggaaataaa gattctcaat
     2041 gatgagattg agaagagtgg aacccttgaa ttcattgaga aggtggcaag ggatgacctt
     2101 ggcatggtta agccgcgaga gataatctat gtggataaaa gtctggaagg aaacaataca
     2161 ctggaaatct ttaagaatga taactgattg acaggggcca tccagtaata tatacttgac
     2221 gtatattaaa catatattaa aaattttaag gaggagtaat ttgtctatgt ccgtttcagt
     2281 tggagagatc gttgagggta cagtaacagg gattacaaat tttggcgcat ttgtacagtt
     2341 gccagaaggg aagagtggac tggtacatat atcagaggta tcccacgact acgtagaaaa
     2401 agtatctgat taccttaaga aggatcagaa aataaaagta aaggttttat caatatccgc
     2461 agatggaaag atcagtcttt ctgtgagaca ggcaaaacct aagacaagta agccagtgga
     2521 aatcgattgg gcaaaatcgg atgacaggca aaagaacatg tcctttgaag atatgttgaa
     2581 cagctatctt aaggatagca gcgaaaagat ggatcagatt aaaaccaaag atggcagaaa
     2641 aagctatgga atgaaaggca aaaaagccag taacaacgat taataaaaac cgggttttcc
     2701 ggttttttct gtttttatta caaataggaa atttctactt ttttcacaca ggtattaaaa
     2761 ctatttgacc atagggtgat gagcatgata gatcttgtaa ggagtaatat cacaaggcat
     2821 aaattgctgg aaaatatcga tacagtactt gccgcagttt ccggggggcc ggattcaatg
     2881 gccatgctgt attgtctcta cgagctcaga gaccagtttg aatttaagct cttggtcgcc
     2941 catgtgaatc atggggtacg tggagagctt gccaggagag accagaattt cgttgagaaa
     3001 atctccaagg accttggact tgactactat acaactaatg tagatatggc tgcctatgga
     3061 aaagcccatg ggatgacctc tgaagaggct ggacgcttgc tcaggtatgg ctttttcagg
     3121 gatgtattag aggaaaatgg cggcggtaga atcgccgttg cacataataa gaacgaccag
     3181 gctgaaactg ttcttcacag gataataagg ggaactggcc tggatggaat cagggccatg
     3241 tcaatgataa gtggcgacat catccgacca ttgctgaaca tatcaaggga tgatatcgaa
     3301 agctttattg aggatagggg aatagcttca gttcacgacc atacaaacct tcaggccgtc
     3361 tacaccagaa acaagataag gcttgagctt ttaccatacc taagcgacaa tttcaaccca
     3421 aacatagtgg actccctata caggctttct gagattgcac aacttgacct ggaggtactg
     3481 gatcgggaga ttgagaaaaa gtataattta ctagtgaaaa aaagaacaaa caatagtata
     3541 atctttaaag gtgaacagtt tatcaaggaa gatgaaggtt cggccaggag gcttgtaaga
     3601 agagcaatac tggatgtggt aggtgacttg aatggttttg gagaggtaca cattcaaagt
     3661 gcagccaata tcttcttgac aggtgacacc gggaaatccg tccatttggg aagaaatgta
     3721 atggcagagg tatcctaccg tgactttgca attaaagttg gagggaccga aagcttgaag
     3781 tttca
//
LOCUS       DASRQF010000306         4639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_55835, whole genome shotgun sequence.
ACCESSION   DASRQF010000306 DASRQF010000000
VERSION     DASRQF010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55835"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VFD79_08205"
     CDS             <1..552
                     /locus_tag="VFD79_08205"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HZH94074.1"
                     /translation="RTVDLPVNLITINELPDNYAITDIEISPGTVAVKGDNSITQLTE
                     LRTLEVDINTMLENESIEVELDLPEGVTLLDPDQVIRVDYQVREVVTTTLEFSFEDIE
                     FRNLQGGLELEPVGQNQTYTLELTGYDSDMEEISLEDLALFVDLEGYDAGNHEIEISW
                     EEMDNLSIGNLQPPIVQINITEE"
     gene            577..1659
                     /gene="buk"
                     /locus_tag="VFD79_08210"
     CDS             577..1659
                     /gene="buk"
                     /locus_tag="VFD79_08210"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HZH94075.1"
                     /translation="MERKYVLAINPGSTSTKVSVFMGQNEFRTKKLDHGLEDLNRFES
                     IADQYDYRLNIILDWLDEENIQLEELECVVGRGGLLKSMPSGTFKVTDEMAKDLKIGI
                     QGQHASNLGGLLARGIGEKEGIPAFIVDPVAVDEFHDVARISGIKAIERKSLVHALNI
                     KAVCHRYATENNTSVDKLNVIVAHLGGGITVSPILEGKIVDANNANQMGPFSPERAGG
                     LPVGDIIGMCFSGEYTEQDMKKMIQGKGGLMSYLGTFDGREVMERINSGDEYAKFIYE
                     AMAYQIGKEIGLCGPVLKGEIQGIILTGGLAYSDYLVDYIKDMVSFMAPVTVYPGEDE
                     MEALNKGARRVMDGEESFKIYEEEVE"
     gene            1659..2555
                     /locus_tag="VFD79_08215"
     CDS             1659..2555
                     /locus_tag="VFD79_08215"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HZH94076.1"
                     /translation="MAKNFESLFKLAQEKGPKKMAVAVAQDEDVLGAIKLAYEKGIVE
                     PILVGDSELIQKLAAGISFDISNVQIIDEKDGVEATRKATALVSSGEAQVLMKGLIDT
                     SVIMKAVLDKEIGLRTDSVISHVAVFDVPTYHKVFMVTDAAMIIAPDLDQKKKIIENA
                     VKLAHSLDIAEPKVAVLAAKEKVSPKMEATVHAKELADMNKAGEISGCIVDGPFALDN
                     AVSKESARIKGIDSVVAGDADILLAPDIEAGNVLYKALSFLANAKSAGLIVGTSKPIV
                     LTSRADNEEAKLNSIVLATLMA"
     gene            2568..3641
                     /gene="buk"
                     /locus_tag="VFD79_08220"
     CDS             2568..3641
                     /gene="buk"
                     /locus_tag="VFD79_08220"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HZH94077.1"
                     /translation="MENYRLLVINPGSTSTKIGVYENEKLLFEETLRHDVGELSKFHK
                     MIDQFEFRKEIIIGALKKNKIEIESLHAIVGRGGLLKPIEGGTYEVNEEMLKDLRKAA
                     RGEHASNLGAILAYEIARTANGRAFIVDPVVVDELQDVARISGLKEIERKSILHALNQ
                     KAVARRYAKEQNKKYTDLNLIVAHLGGGISVGAHRKGQVVDVGNALDGEGAFSPERSG
                     GLPIGDLIKLCFSGKYTLDEIKKMIKGNGGLVSYLGTNDGREVVKRINEGDEYAELIY
                     RAMAYQVAKDIGASAAVLKGDVDQILITGGIAYDDMFTGLIKEMVEYISGVTVYPGED
                     ELEALAEGGLRVLRNEESPKVYS"
     gene            3651..4325
                     /locus_tag="VFD79_08225"
     CDS             3651..4325
                     /locus_tag="VFD79_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZH94078.1"
                     /translation="MEKDNRIRIIIGHYGSGKTEFSVNYALKLAQMGKKVALADLDVV
                     NLYFRSREKAEILEEKGIRVIGGAIKGSGMDLPAISGEVLTPLQDKSYQAILDVGGNP
                     QGARTLGRYHTYFKDGEYDMLFVLNANRPETQTVDQVEEYIVKIQDVARAKVTGIINN
                     THLLKSTTVEDVMRGYNLAREVSTRTGLPIKYNACLETLVDELPEELGDNVFPIKLYM
                     REEWML"
     gene            4345..4572
                     /locus_tag="VFD79_08230"
     CDS             4345..4572
                     /locus_tag="VFD79_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZH94079.1"
                     /translation="MAKVKGRTTFEEDLCKGCGLCVTVCPVNIISLDTARINKKGYHP
                     AAIKDEDMDKCIACTNCATICPDVVITVERL"
BASE COUNT         1493 a          789 c         1255 g         1102 t
ORIGIN      
        1 agaacagttg accttcctgt aaaccttata acgataaatg aacttccaga caactatgca
       61 atcacagata ttgaaataag tcctggaaca gtagctgtga aaggggataa ctccatcact
      121 cagcttacag aactgaggac acttgaagta gatatcaaca ctatgcttga aaatgagagc
      181 atagaggttg agttggactt gccggagggc gtgacactcc tggaccctga tcaagtgata
      241 agagtggatt atcaggtcag agaagttgtc acaacaacac ttgaattttc atttgaggat
      301 attgagttta gaaatcttca gggaggcctg gagcttgagc cagtgggcca gaaccagaca
      361 tatacattgg agttgacagg ttatgattct gatatggaag aaatcagcct ggaggacctt
      421 gctctgtttg ttgaccttga aggctatgat gcaggaaacc atgagattga gatttcgtgg
      481 gaggaaatgg ataacctgtc tatcggcaat ttacaaccgc cgatagttca gataaatatc
      541 actgaggaat aatattcact taacgagggg gatgctatgg aaaggaaata cgtattggcc
      601 atcaatccgg gatccacatc aacaaaggtt tccgtattta tgggtcaaaa tgagtttagg
      661 actaaaaagc ttgaccatgg gttggaagac cttaacaggt ttgaatcaat cgctgaccag
      721 tatgactaca gacttaacat catacttgat tggcttgatg aagaaaacat acaactagag
      781 gaactggagt gtgttgtagg aaggggagga ctactgaagt ccatgccaag tgggaccttc
      841 aaggtgactg acgaaatggc caaggacctg aaaataggaa tccaaggaca gcatgcatcc
      901 aaccttggtg gacttttagc caggggaata ggcgaaaagg agggtatacc agcctttatc
      961 gtggatcctg ttgcagtgga tgaatttcat gatgtagcca ggatttcagg gataaaggca
     1021 attgaaagga agtccctggt ccatgcattg aacataaagg cggtttgcca caggtatgcc
     1081 actgaaaata atacaagtgt tgataagctt aatgttatcg ttgcccatct tggaggcgga
     1141 ataacagttt cccccatact ggagggaaaa atcgtggatg ccaacaatgc gaatcagatg
     1201 ggaccgtttt cccctgaaag agcaggaggt ttgccagtag gtgatattat tggaatgtgc
     1261 ttttcaggag agtatacaga gcaggatatg aaaaaaatga ttcagggaaa aggtggactt
     1321 atgtcctatc ttggtacctt tgacggccgt gaggtcatgg aaagaatcaa ctcaggagat
     1381 gaatatgcaa agtttatata tgaagcaatg gcatatcaga taggcaagga gatcggtctt
     1441 tgtggtccgg ttctcaaagg agagatccaa gggataatcc taactggtgg cttggcatat
     1501 tcggattatc tggttgacta tatcaaggac atggtgtcat ttatggcacc ggtaacggtt
     1561 tatcctggag aggatgaaat ggaggccttg aacaaggggg ccagaagagt gatggatggg
     1621 gaagaaagtt tcaagatata tgaggaagag gtggaataat ggcaaagaat tttgaaagtt
     1681 tattcaagct tgctcaggaa aagggaccaa agaaaatggc tgtagctgtt gcacaggatg
     1741 aggatgtatt gggagcgatc aagctggcct atgaaaaagg tatagttgag ccaatccttg
     1801 tgggagatag tgaactgatc caaaagttgg ctgcgggtat atccttcgac atttcaaatg
     1861 tacagataat cgatgagaag gatggagttg aagcaaccag aaaggcaacg gccttggtaa
     1921 gcagcggtga ggctcaggta ctgatgaagg gtctgataga cacatccgtg atcatgaaag
     1981 cagttcttga caaggagatt ggtcttagaa cagatagtgt aataagccat gttgctgtat
     2041 ttgatgttcc aacctatcac aaggtattta tggtgacaga tgccgctatg ataatagcgc
     2101 ctgatttgga ccagaagaag aaaatcattg aaaatgctgt taagctagcc cattcattgg
     2161 atattgcaga gccaaaggta gctgttcttg ctgccaagga gaaggtgagt ccaaagatgg
     2221 aggcaactgt ccatgcaaag gaactggctg atatgaacaa ggcaggagaa atatcaggat
     2281 gcatagtaga cggtcctttt gcccttgaca atgcagttag caaggaatcg gcaagaatca
     2341 aagggataga cagtgtggtt gcaggagatg ctgacattct acttgcaccg gacattgaag
     2401 caggaaatgt actatacaag gcattgagct ttttggcaaa cgcgaaatca gctggcctga
     2461 ttgtgggcac aagcaagccg attgttctga catcaagagc agacaacgag gaagccaagc
     2521 ttaattcaat tgttctggct accttgatgg cataagggga ggaatttatg gaaaactaca
     2581 gattattggt gataaacccc ggatcaacat caacaaaaat tggggtttac gagaacgaaa
     2641 agcttctttt tgaagagaca ctaagacatg atgtgggtga gctttccaaa ttccacaaga
     2701 tgattgacca gttcgagttt agaaaggaaa taataattgg agctcttaaa aagaacaaga
     2761 tagaaattga aagtctccac gcaatagtgg ggagaggggg gctactaaag ccaattgaag
     2821 gtggcaccta cgaggttaac gaggaaatgc tcaaggacct tcggaaagcc gcgagagggg
     2881 agcatgcatc aaatcttgga gccattctgg catacgaaat cgccaggact gcaaatggca
     2941 gagcatttat tgtggaccct gtggttgtgg atgagttgca ggatgttgca agaatttccg
     3001 gcttgaagga aattgagaga aaaagcatac ttcacgccct taaccaaaag gcagttgcta
     3061 gaaggtatgc aaaagagcag aacaagaaat atactgacct gaaccttata gttgcccacc
     3121 ttggtggagg aatatctgtg ggagcccaca gaaaaggtca agtggtggat gtaggaaatg
     3181 cccttgacgg tgaaggcgcc ttctcaccgg aaagatcagg aggccttcca attggagacc
     3241 ttataaagct ttgcttttca ggaaagtaca cacttgatga gatcaagaaa atgataaagg
     3301 gaaatggtgg gctggtatcc tacctgggaa caaacgatgg tcgagaggtt gtaaagagga
     3361 tcaatgaagg cgatgagtat gcagagttga tctacagagc catggcctac caggtggcaa
     3421 aggatattgg agcgtcagca gcggttctta agggagatgt agaccagatc cttatcacag
     3481 gggggatagc atacgatgat atgtttaccg gtttgatcaa ggaaatggtt gaatatataa
     3541 gtggtgtcac tgtctatcct ggagaggatg agcttgaggc acttgctgaa ggtggcttga
     3601 gagtgcttag aaatgaggaa tctcccaagg tttacagtta ggagtatgga atggaaaaag
     3661 acaatagaat cagaatcata ataggacatt atggaagcgg caagactgaa tttagtgtaa
     3721 attatgccct taagctggct caaatgggca agaaggtagc acttgctgac ctggatgtgg
     3781 taaacctgta tttcagaagc agggaaaagg cagaaatcct tgaggaaaag ggaattaggg
     3841 taattggcgg agccataaaa ggctccggca tggaccttcc agcaatatca ggtgaggtcc
     3901 ttacacccct acaggacaag tcctaccagg caatacttga tgtaggtgga aaccctcagg
     3961 gagcaagaac ccttggaagg taccacacct atttcaagga cggagagtac gacatgctct
     4021 tcgtactgaa tgcaaaccga cctgagaccc agaccgtgga ccaggttgag gagtacattg
     4081 taaaaataca ggatgttgca agggctaagg ttacaggaat cataaacaat acacatcttt
     4141 tgaaaagtac aacagtggag gatgtcatga gaggctataa tttggccagg gaggtttcaa
     4201 ccagaaccgg cctgccaatt aagtataatg cttgtctcga gactctggtg gatgagctgc
     4261 ctgaggaact tggagacaac gttttcccga tcaagcttta tatgagggaa gaatggatgc
     4321 tataaaaaaa ggaggatcag aaatatggca aaggtaaaag gcagaacaac atttgaggag
     4381 gacctgtgca agggctgtgg actttgtgtt acggtttgtc ctgtaaacat catttcactt
     4441 gatactgcta gaatcaacaa gaaagggtat cacccagcag ctataaagga tgaagatatg
     4501 gacaagtgta ttgcatgtac aaattgcgct acaatatgtc cagatgtggt tattactgta
     4561 gagagattat aaaggagggg ttacatatga caaaagttct catgaaagaa aatgaagcag
     4621 taggagcagc tgcaattgc
//
LOCUS       DASRQF010000307         5906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_429823, whole genome shotgun sequence.
ACCESSION   DASRQF010000307 DASRQF010000000
VERSION     DASRQF010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429823"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            384..1982
                     /locus_tag="VFD79_08235"
     CDS             384..1982
                     /locus_tag="VFD79_08235"
                     /inference="COORDINATES: protein motif:HMM:NF014683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HZH94080.1"
                     /translation="MNMEFSFQLWLVAALPILLLLLFMVGLKWPALKAAPVSLLVTLL
                     TASFVFKATPELILIEAGKSIWSSLSIISVVITAILLYETSKEAKAFDTLNKAFSVSA
                     PNELLRILLIGVVFSSFLQGVTGFGVPILVTAPLLIGIGVSPMWAVVVPLIGHCWAGT
                     FGTLALAWHALILQTDLVSTVWVALYASVLLSILNLSAGIAIAWFYGRFEAIGKGLLA
                     ILAISITQGFGQIVMTQVNPDLGVFVPSAASAGILILVSKMNMYRDGWSVKNSRIMEP
                     SAANEPIEGSNAMSAFQAFFPYLAMTFITLSILMISPLNSFFGSLSYGPSFPGSVTGL
                     GVHNAPVAVYAPIKPFTHASFFLALSALMGYVFYQKRSFVRTGSLSLILSRALRKALP
                     SSLAILSLMGISRIMTGSGQTEILSYGVASLFGNLYAGISPFVGLVGAFITSSNMSSN
                     ILFGGFQYSLSEILQMDSYLLLGAQTAGGSIGTSIAPSNVVLGATTAGILGKEGDILR
                     KIIPFTIAVTSIFGITTLVLTLYL"
     gene            complement(2031..3713)
                     /locus_tag="VFD79_08240"
     CDS             complement(2031..3713)
                     /locus_tag="VFD79_08240"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZH94081.1"
                     /translation="MKFDSSVFDFMKKMTKIDINLKNRKWNLNNNRFRRFLAVLTVVA
                     VVGVSYVSYSVNDTRTKAFDVYLGDDHIGVVRTEEEALEVYRDLEESLKSTYDMGVVL
                     DKELEFKETNAKDEALSTANEIKSNIKDNINVSVAGFELVVDGESFGIFRSGQEVEYI
                     LDTIKSSYLPQVVEEEAIVAAGNQVEEVSAPRTHTVEDGDNYWTIARQYNMTATALEE
                     ANPGVNPSRLKPGDIINLTEGGQAQDEVKVQNDFPVVDKDADIKEVDFSEDIEVVKVE
                     TSFNKLADPDETIARIREGKEEMRTHIVEVGESYWIIARMYDTTVEELLSANPGQDSQ
                     SLKPGDEVNLFVPTPLLTVRTVEQIQYTEDTDFETEVKTDDSMYKNQKKILTKGEKGK
                     SEIVANVTKLNGIVEEKEIINEKVIVEPVTEVVVQGTKELPKTAATGSFMVPTSGRLS
                     SPYGMRGGSMHRGIDLANSTGTKIFAADGGRVTFAGYKGSYGYMIEINHENGYVSRYA
                     HLSKIYVKSGDRVYRGQHIAGMGSTGRSSGPHLHFEILLNGSHQNPSRFINR"
     gene            complement(3992..4870)
                     /locus_tag="VFD79_08245"
     CDS             complement(3992..4870)
                     /locus_tag="VFD79_08245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HZH94082.1"
                     /translation="MDRYLFIINPTAGSGRTKAYTDFVENYMSSTGREYRVEVTTKPL
                     EATELVMKFPQYNICVAVGGDGTANEVAKGILKRGSGTLGIVPSGTGNDIAQALHLNM
                     DREKAMERVLKGKVASIDLGMVRGDYFFNIASVGFDAWVVKETDRIKKVIKGKLSYVL
                     GVLMGLLTYRSREVHLEIDGESIKRRATLVAVGNGQSYGGGMKILPMASIYDDMLDVC
                     VVKDISNLKILFLFPSIFKGEHTRYVKHVEFFKGRNIKVSLNKEELLNIDGELFEVED
                     EVVEFEISHQKLSVIV"
     gene            complement(4883..5401)
                     /locus_tag="VFD79_08250"
     CDS             complement(4883..5401)
                     /locus_tag="VFD79_08250"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HZH94083.1"
                     /translation="MIQVLEFIEYNSTYFIMGTLFALLIMIILYVTQIREYHKHKERY
                     DSLTRGLSGANIEDLFIKINTELSSLNRDINLIEDNIEALETKLTFTIQKMGFVRYNA
                     FADTGSELSFSIALLDAYNNGFVLTSIYGRDQAVMFAKPIQNANCNVPLSPEEMAAIN
                     RALMGESAEKIL"
     gene            complement(5412..>5906)
                     /locus_tag="VFD79_08255"
     CDS             complement(5412..>5906)
                     /locus_tag="VFD79_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZH94084.1"
                     /translation="HVDQFIQGGTGSASYSLKQPEEMPDKFESGTPNAPGIIGLGRGV
                     QYIKDRGIDNIRHHEEDLTGYFIDEASRIDGINLYGPLDTSKQGAVVALNIRDLDSSE
                     VSYVLNEEFGIYVRPGLHCAPLAHETIGTLEQGAVRFSFGPLNTKDELEYAIKALHRI
                     SKEL"
BASE COUNT         1446 a         1439 c         1084 g         1937 t
ORIGIN      
        1 cccatcatta ttgatacttt tcgtacacct tccttctcag ttagccttgt acttagcagc
       61 atcaagttaa ctgagtcctg atatgtattc cttttcaaaa gtcctagtat catgcattct
      121 ttcctcccta ttatttaata acttactccc aaacatataa gacctgaaaa tcatttcttt
      181 ggttcaatgt aaattttatc accttttaat tacataaacc ttatcagaat ctgataaggt
      241 ttatcggttt tttttcttta tttggatagt ttgtatatca ttaaaggata atttctcgag
      301 gtagtattag tcaatgtttt cattgtataa ggattgaata gattatatga tgagtatgat
      361 aattacaatt tggaggtata catatgaaca tggaatttag ctttcagcta tggcttgtag
      421 ccgctttacc catactcctg ctgctcctct ttatggttgg cctgaagtgg cccgcactta
      481 aagcagctcc tgtttccctt ttggttaccc tattgacggc atcttttgtc ttcaaggcca
      541 cacctgaatt gatacttata gaagcaggaa agtcaatttg gagttcctta agcataattt
      601 cagtggtgat aaccgcaatt ctcctttatg aaaccagcaa ggaagccaag gcctttgata
      661 ccctgaacaa ggcattttca gtttccgctc ccaatgagct cctgagaatt ctacttatag
      721 gagttgtctt ttccagcttt cttcagggtg tgactggatt tggggtcccc atactggtta
      781 cggctcccct tctcataggc attggggttt ccccaatgtg ggctgttgtg gtaccgctta
      841 taggacattg ctgggctggt accttcggaa ccctggcact ggcgtggcat gcattgattc
      901 tgcagactga cctggtttcc acagtatggg tggccctata tgcttcagtg ttgctttcga
      961 tcctaaatct atcagctggt attgcaatcg cctggttcta tggacgtttt gaagccattg
     1021 gaaagggact acttgcaata ctagcgatat ctataacaca aggttttgga cagattgtta
     1081 tgacccaggt aaatcctgac ctgggagtct ttgttccaag tgcggcttcg gcaggtattt
     1141 tgatcctggt atcaaaaatg aatatgtaca gagacggctg gtcagttaaa aacagtagga
     1201 ttatggagcc ttcagctgca aatgaaccaa ttgaaggttc aaatgcaatg agtgcttttc
     1261 aggcattctt cccttatctt gcaatgactt tcataaccct ttcaatactt atgatttcac
     1321 ctttgaatag cttctttggt tcactttcct atgggcccag cttcccagga tccgtgactg
     1381 gcctgggagt tcataatgct ccagttgctg tttatgcacc aatcaagccc ttcacacatg
     1441 catccttctt tctggcacta tctgcattaa tgggatatgt gttctatcag aagagatctt
     1501 ttgtgaggac tggcagccta tcacttatac tttcaagggc gctcagaaag gctcttcctt
     1561 caagcctggc aatactgtcc cttatgggaa tttcacgtat aatgaccgga tccggccaaa
     1621 cagagatcct atcctatggt gttgcctccc tcttcggcaa tctatacgca ggaataagtc
     1681 cttttgttgg gctggtgggt gcctttatta caagcagcaa catgtcttca aacatcctat
     1741 ttggaggatt tcaatactct ttatcagaga ttcttcaaat ggatagctac cttcttcttg
     1801 gagcccagac tgcaggtggc agcatcggga cctccattgc acccagcaat gttgtccttg
     1861 gagctacaac tgccggaatt cttggtaagg agggtgatat cctacgaaag ataattcctt
     1921 tcaccattgc agtaaccagc atatttggaa tcactactct tgtcctaaca ttgtatttat
     1981 aaaataattc aaaaaaagga agtgtggatt ttttccacgc ttcccttgtc ttatctattt
     2041 ataaatctgc ttgggttctg gtgagaccca ttaaggagaa tctcaaagtg caggtgggga
     2101 cctgaagacc ttcccgtact gcccattcca gctatatgtt gacctctgta caccctgtct
     2161 cctgacttaa catatatttt actcaagtgg gcatatctgc taacatatcc attttcatgg
     2221 ttgatttcta tcatatatcc atagcttccc ttgtaacctg caaaggtaac cctgccaccg
     2281 tcggctgcga atatctttgt tcccgtgctg tttgccaagt ctattcccct atgcatgctg
     2341 ccacctctca tgccatatgg agatgatagt cttccgcttg tgggaaccat aaatgaccct
     2401 gtggctgctg ttttcggtag ctcctttgta ccttgaacca caacttcggt tactggttca
     2461 actattactt tctcattaat aatttccttt tcttcaacta ttccattaag ttttgtaaca
     2521 ttagcaacta tctctgactt ccctttttca cccttggtca atatcttttt ctgattctta
     2581 tacattgagt catccgtctt tacttccgtc tcaaaatctg tgtcctctgt gtattgtatt
     2641 tgctcaactg ttctcactgt caaaagcggt gtcggaacaa agaggttaac ctcatcccca
     2701 ggcttaagtg actgtgaatc ctggccggga tttgcactta gcagttcctc aaccgtagta
     2761 tcatacattc ttgcaataat ccagtagctt tcccctacct ctacaatgtg ggttcgcatt
     2821 tcctctttac cttccctgat cctggctatt gtctcgtcag ggtcagcaag tttgttgaag
     2881 ctggtttcca ctttcactac ttctatatct tcactaaagt caacttcctt aatatctgca
     2941 tccttgtcaa caaccgggaa gtcattctgt accttgacct catcttgcgc ttgtccgcct
     3001 tcagttaggt tgataatatc tcccggcttt aatcttgaag ggtttacccc aggatttgcc
     3061 tcctcaaggg ctgttgccgt catgttgtac tgtctggcta tggtccagta attatcgcca
     3121 tcctccacag tgtgtgtcct tggagctgat acctcctcca cctggtttcc tgcagctact
     3181 atagcctctt cctctacaac ctggggaaga tatgaggatt ttattgtatc cagtatatat
     3241 tccacttcct ggcctgatct gaagatccca aaactttctc catctacgac tagctcaaat
     3301 ccagcaacgc ttacatttat attgtcctta atatttgatt tgatttcatt tgccgttgac
     3361 aaggcctcat ccttggcgtt tgtctcttta aactctagct ctttgtcaag tacaactccc
     3421 atgtcatatg tactcttgag actttcttcc aggtccctgt aaacctccag tgcttcttct
     3481 tcagtcctga caacccctat atggtcatct ccaagataaa catcgaatgc cttggttctg
     3541 gtgtcgttta ccgagtagct tacatagctt acaccaacaa cagccaccac agtcaaaact
     3601 gccaaaaatc tcctgaatct attattgtta aggttccatt ttctattttt caaatttatg
     3661 tctattttgg tcatcttctt cataaagtca aatactgagg aatcaaactt catcaataaa
     3721 tcgcctccga gctgcgccct ccatccgggc ttttatttgt atccgatagg tggttacaat
     3781 atttgtaacc gttttgtaac attaacataa acattatacc aattgggcta cataaataca
     3841 acctgcctaa ggctgtttcg ctgtgcagtg gtaatgaaag tgttttcccg taaaaaaacc
     3901 ccttatcact gtcatagtgt ggggtttgtg catgtcaaaa tatggaattt cctgaaaatt
     3961 aaatttatga acaaaattta acattatttc atcacactat cacgcttagc ttctggtggg
     4021 atatttcaaa ctcaaccacc tcgtcctcca cctcaaaaag ctctccatct atattgagaa
     4081 gctcttcctt gttcaaggac accttgatgt tcctgccctt gaaaaactca acatgcttca
     4141 catacctggt atgctcaccc ttgaaaattg atggaaaaag gaacaggatc ttaaggttgc
     4201 tgatatcctt aaccacgcac acgtccagca tatcatcgta tatgcttgcc atgggaagta
     4261 tcttcatgcc tcccccatag gactgtccat tccccacagc aacaagggtc gccctccttt
     4321 tgatggactc tccatctatt tccaggtgaa cttccctgct cctataggtc aggaggccca
     4381 tgagaactcc cagtacatag gatagctttc ccttgatgac cttctttatc ctgtcagtct
     4441 ccttcacaac ccaggcatca aatccaacac ttgcaatatt gaagaagtag tctcccctga
     4501 ccatcccaag gtctatgctt gccactttac ccttaagcac cctttccatg gccttttccc
     4561 tgtccatgtt aagatgaagg gcttgggcaa tatcattgcc tgttccggag ggaactattc
     4621 ccaatgtccc ggacccacgt ttaaggatcc cctttgcaac ctcattggct gtaccgtctc
     4681 ccccaactgc cacacagata ttgtattgtg gaaacttcat taccagctcc gtggcttcca
     4741 gtggcttggt ggtaacttct accctgtact ccctacctgt gcttgacatg tagttctcaa
     4801 caaagtctgt atatgccttt gttcttccac ttcctgcagt aggatttatt ataaaaagat
     4861 atctatccat aagcttctcc tgttacagta ttttttcagc gctctccccc ataagtgccc
     4921 tgtttatggc tgccatctct tcaggtgata atggcacatt gcagttggca ttttggatag
     4981 gcttggcaaa catcactgcc tggtctcttc cgtatatgct ggtaagtaca aatccattgt
     5041 tgtaggcatc aagcaaggct atggaaaagc taagctcact tccggtatct gcgaatgcat
     5101 tgtaccttac aaagcccatc ttctgaatgg tgaaggtgag ctttgtctca agagcttcaa
     5161 tattgtcctc tatcaggttt atatccctgt ttaggctgct tagttcagta tttattttta
     5221 tgaaaaggtc ctcgatattg gctcctgaca atcccctggt cagggaatcg tacctttcct
     5281 tgtgcttatg atattccctg atctgtgtaa catataggat aatcataatt aaaagtgcaa
     5341 acagtgttcc catgataaaa taggtagagt tgtattcgat aaattccaga acctgtatca
     5401 tcccatcact cctatagttc ttttgaaatt ctatggagag cctttatggc atattcaagc
     5461 tcatcctttg tattcaatgg accaaaactg aatctcactg ctccctgctc caatgtccca
     5521 atggtctcat gtgccaaagg tgcacagtgc agccctggcc ttacatatat tccgaactct
     5581 tcattcagaa cataggacac ctctgatgag tccaggtccc tgatattcag agcaacaact
     5641 gctccctgct tggatgtatc aagtggacca tataggttta ttccgtcaat tctggatgct
     5701 tcatcaatga aatatcctgt aaggtcttcc tcgtggtgtc tgatattgtc aattccccta
     5761 tccttgatgt actgaacacc cctgccaagg ccaattattc caggagcgtt gggcgttcca
     5821 ctttcaaact tatcaggcat ttcctcaggc tgtttcaagg aataggatgc actaccagta
     5881 ccaccttgga taaattgatc tacatg
//
LOCUS       DASRQF010000308         2641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1101664, whole genome shotgun sequence.
ACCESSION   DASRQF010000308 DASRQF010000000
VERSION     DASRQF010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101664"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..2158)
                     /locus_tag="VFD79_08260"
     CDS             complement(230..2158)
                     /locus_tag="VFD79_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZH94085.1"
                     /translation="MPRPMGRGPRGMGPAKIKDINKGTVKRLLSYLGKYKVRLVFVVV
                     SIIISAFTGAIASLFLRTLIDDYITPMLIQANPDFSGLFRVILMMGGIYILGVLASLF
                     YTRSMVTISQGVLKRIRDELFSKMQSLPIRYFDSRSHGDLMSHYTNDIDTLRQMLSQS
                     LPQMFSSVVTIVAVMTAMLYLSLWMTGFVILFVFIVLKVVKTIAGKSGKYFVEQQKSL
                     GDLNGYIEEMINGLKVIKVFNHEEVVKSDFDKKNELLRENSTKANQYANILMPIMMNL
                     GYVLYVLLAIVGGVLAVGGVQNYSITGVDIITLGMIASFLQLSRNFIQPIAQVSQQLN
                     AVVMAIAGAERIFDLMDQEPEEDEGYVTLVNTSYENGKIVEANERTHLWAWKHPHGDG
                     TVTYTPLKGEVRFYDVDFGYNEDELVLHDITLYAEPGQKVAFVGATGAGKTTITNLIN
                     RFYDIADGKIRYDGINIKKIRKADLRRSLGIVLQDVNLFTGTVMDNIRYGRLDATDEV
                     CIEAARLANAAGFIRMLPDGYQTVLTGDGGGLSQGQRQLLSIARAAVADPPVMILDEA
                     TSSIDTRTESIVQRGMDALMKGRTVFVIAHRLSTVQNSNVIMVLEKGRIIERGSHDEL
                     VQEKGKYYQLYTGAFELD"
     gene            complement(2151..>2641)
                     /locus_tag="VFD79_08265"
     CDS             complement(2151..>2641)
                     /locus_tag="VFD79_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004834000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZH94086.1"
                     /translation="KNVLFSGTIRENLRWGDENASDEEIKRMADLAQADEFIRDFPDN
                     YDTHIEQGGSNVSGGQKQRLTIARALLKRPKIIILDDSTSAVDTRTDALIKRAFMEEI
                     PDTTKLIIAQRVSSVQDADRIIVMDDGEIKAIGTHDELLVDSDIYREVYESQVKGGMA
                     SA"
BASE COUNT          642 a          698 c          504 g          797 t
ORIGIN      
        1 cctgtaggtc ccgaagtctt ccttccaatt ccgcttcccc ttttctccac tctggagctt
       61 cctgtaccga ctctcttttt tctacctgaa ggcctgtttt ttctatccat atttttcatc
      121 tcctaggttt ttattacaag actttatatt tatatatacc cctatggaat aatttttgga
      181 cacaaaaaag cagccaatac cattttattg gcaaaggctg catattttct tagtcaagct
      241 caaatgcacc tgtatagagc tgatagtatt tacccttttc ctgaaccagt tcatcgtggc
      301 taccccgctc aatgatcctt cccttttcaa ggaccattat gacatttgag ttctgtacgg
      361 ttgaaagcct gtgggcaatt acaaacactg tcctgccctt catcagggca tccatacccc
      421 tttgaacgat agattcagtt ctggtgtcta ttgaggatgt cgcctcatca agaatcatga
      481 caggtgggtc tgcaaccgca gcccttgcta tggaaagaag ctgtctctgt ccctgggaaa
      541 gtcctccgcc atccccggtc aagactgtct ggtagccgtc aggcagcatc cttataaatc
      601 cagcggcatt ggcaagcctg gcagcctcaa tgcacacctc atccgttgca tccagtcgtc
      661 cgtaacggat gttgtccatt accgtacctg taaagaggtt gacatcctga agtacaattc
      721 caagggacct tctaaggtca gccttcctga tcttcttgat gttaattcca tcatacctga
      781 tctttccgtc agcaatatca tagaatctgt tgatgagatt agttattgtg gtcttgccgg
      841 cacccgtagc tccaacaaat gccactttct gtccgggctc agcatacagg gtaatgtcgt
      901 ggaggaccaa ctcatcctca ttgtatccga aatccacatc atagaacctc acctcaccct
      961 taaggggtgt ataggttaca gtgccatcac cgtggggatg cttccatgcc cacaaatggg
     1021 tcctttcatt tgcctcgaca atcttaccgt tttcatagct ggtatttaca agggtcacat
     1081 acccctcatc ctcttcaggc tcctggtcca tcaggtcaaa tatcctttca gcacctgcga
     1141 ttgccattac tacagcatta agctgctggg aaacctgggc gatgggctgt atgaagtttc
     1201 ttgacagctg caaaaatgat gcaatcatac caagtgttat tatgtccaca cctgttatgc
     1261 tgtagttctg aactccccca acggccagaa cccctccaac tatggcaagc agtacatata
     1321 gcacatagcc caagttcatc attattggca taagaatgtt tgcatactgg tttgcctttg
     1381 tggaattctc ccttaggagc tcattctttt tatcaaaatc acttttcaca acctcttcat
     1441 gattaaacac ctttataacc ttaaggccgt ttatcatctc ctcgatgtat ccgttaaggt
     1501 ctcccaatga tttctgctgc tccacaaagt atttgccact tttacctgca atggtcttta
     1561 caaccttaag gacaatgaag acaaacagaa tcacaaatcc tgtcatccaa agactcaggt
     1621 atagcatggc agtcattacg gccacaattg taaccactga tgaaaacatc tggggaagac
     1681 tctgtgacag catctgcctc aatgtatcaa tgtcatttgt gtagtggctc atcaggtctc
     1741 catggctccg tgagtcgaaa taccttatgg gtagactctg catttttgaa aacagctcat
     1801 cccttatcct cttcagcacc ccctgggata ttgtaaccat tgacctggtg tagaacaggg
     1861 atgccaacac acccaggata tagattcctc ccatcatgag gattaccctg aacaggcctg
     1921 aaaagtcagg attagcctgt atcagcattg gtgtaatgta gtcgtcaatc agggtcctta
     1981 gaaacaggga ggctattgcc cctgtaaagg cactgattat tattgaaaca acgacaaaaa
     2041 caagccttac cttatatttg ccaagatagg acagcaacct ctttacagtg cccttgttga
     2101 tgtccttgat ttttgcaggt cccattcccc ggggacctct tcccattggt ctaggcacta
     2161 gccatccctc ccttcacctg tgactcataa acctctctgt atatatcact atccacaagc
     2221 agctcatcat gagtgcctat ggcctttatc tctccatcat ccatgacgat aatcctgtcg
     2281 gcatcctgga cagaggatac cctctgggca attataagct ttgtggtatc tggtatctcc
     2341 tccatgaagg ccctttttat gagggcatct gtccttgtat ccactgcact tgtggagtca
     2401 tcaagtatta tgatctttgg tcttttaaga agggctcttg ctatggtcag cctttgcttc
     2461 tggcctccgg agacattgct tcccccctgc tcgatatgtg tatcgtaatt gtcagggaag
     2521 tctcttatga actcatcggc ttgggcaagg tctgccatcc tttttatctc ttcatcgctt
     2581 gcattttcat ctccccatct gaggttctcc ctgatggttc ccgagaaaag cacattcttc
     2641 t
//
LOCUS       DASRQF010000309         2340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_849836, whole genome shotgun sequence.
ACCESSION   DASRQF010000309 DASRQF010000000
VERSION     DASRQF010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849836"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VFD79_08270"
     CDS             <1..470
                     /locus_tag="VFD79_08270"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZH94087.1"
                     /translation="DAGVEVFFRQIALTKSVFISRGDQSGTHEKELELWSHAKIAPEE
                     RNIINGGSMASNKECIGISLEKSAYTLVDSTSYKLSEHKNDLRIYAGNRSGIDPGLDN
                     IFSAIAVSRLPQDSARYSEAVRFINWLTGPEARSIIEGYLDREGQEPYFHLIY"
     gene            complement(552..764)
                     /locus_tag="VFD79_08275"
     CDS             complement(552..764)
                     /locus_tag="VFD79_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004836372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HZH94088.1"
                     /translation="MKLSAKNQFKGKITDIKEGVVTGIVTVDIGGGNIVTSSITMDSI
                     KSLDLKVGKEVLTLVKASNVMIGLDD"
     gene            919..2013
                     /locus_tag="VFD79_08280"
     CDS             919..2013
                     /locus_tag="VFD79_08280"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate/molybdate transporter"
                     /protein_id="HZH94089.1"
                     /translation="MREKDFRFTLDEAAGALGDFGTLLPIVVGVSVATDMDLSRMLFF
                     FALAYFGTGLYYKMPMPVEPMKAIGVVAIAEGLSAAEIAGAGIGMGIILFIIAVTGLM
                     GWIKRIMPTRLIRGIQFGLALTLGWQAIKMIWGDPVMGVISMIIVIFYAKSTRLDISA
                     LIVFGLGIGVGIAKFGAPEISMMTMPELIIPDAASMVNGIVKGTLPQVALTLGNAVLA
                     TALLISDLLDRKVKEKELLLSMSAMCLLSTPFGGFPMCHGAGGLVAQYRFGARTGGSN
                     IISGTILLVVAILFATPHLELIIPFGALGAMLFYSAIALYRTATKTEDMSFTAITGVI
                     AFFLGMAWAFVIMLLAHNIQKRIKGVDSQK"
     gene            2063..>2340
                     /locus_tag="VFD79_08285"
     CDS             2063..>2340
                     /locus_tag="VFD79_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH94090.1"
                     /translation="MKFLNVSKENMDIDGIPAIVLRPVLEMGSYPTVVFYHGWSSKKE
                     YQEFRGAVIASLGYQVIIPDALHHGERGELDYAATENAGFFWRVVLTNL"
BASE COUNT          677 a          433 c          563 g          667 t
ORIGIN      
        1 aggatgctgg agtagaggtc tttttcagac agatagccct gacaaaatca gttttcataa
       61 gtcgtggaga ccagtcaggg acccatgaga aggagcttga gctttggagt catgcaaaga
      121 ttgctcctga agaaagaaat ataataaatg gtggaagcat ggcaagcaac aaggaatgta
      181 ttgggatttc tcttgagaag tcggcataca cccttgtgga ctcaacaagc tacaagcttt
      241 cagaacacaa gaatgatctg aggatatatg caggaaacag gagtggaatc gatccagggc
      301 ttgataacat attttcggca atagcagtca gtaggcttcc acaggattct gccaggtaca
      361 gtgaagctgt aaggtttata aactggctaa ctggtcccga agcaagaagc ataattgaag
      421 ggtatttgga cagggaaggt caggagcctt actttcattt gatatattaa aaaggaccaa
      481 ggatgcaaga atcacatcca aggtccctaa taaactgcta tagttttgtt gtgaaaatag
      541 acaggtcagt attaatcatc cagcccaatc attacatttg aagctttaac cagggtaagg
      601 acttccttgc ctaccttaag gtcaagtgac ttgatggagt ccatggtaat ggatgatgtt
      661 acaatgttac ctccgccgat atccacagta actattcctg ttacaacacc ctccttgata
      721 tcggttattt tacctttgaa ttgatttttg gcacttaatt tcatataatc cctcctagaa
      781 taatctaccc cctatatacc ctttaagggt aaagggtatt cacaggtttg aatttttggt
      841 aaaattcaca gagggctaaa tttttataat aatagattag tgggttaaac ataatccatt
      901 ttgtcaggag tgaatatcat gagagaaaag gactttagat ttacattgga tgaggcggca
      961 ggagccctgg gagattttgg aaccctgctt ccaattgttg ttggcgtatc tgtagcaact
     1021 gatatggacc tgtccaggat gctcttcttt tttgcccttg cgtattttgg gactggactc
     1081 tattacaaaa tgccaatgcc cgtggaaccc atgaaggcca taggggtagt cgccatagca
     1141 gagggattgt cagcagctga aattgcagga gcgggtattg gaatgggaat aattctattc
     1201 attattgcag ttaccggatt gatgggctgg atcaagagga taatgcctac acgattaata
     1261 cgtggaatac agtttggtct tgcattaacc ctaggctggc aggcaatcaa gatgatctgg
     1321 ggagacccgg ttatgggcgt gatttccatg ataatagtaa tattctatgc caaatcaacc
     1381 aggttggata tttccgcatt gattgttttt ggactgggta taggggtagg tattgctaaa
     1441 tttggtgcac ctgaaatatc aatgatgacc atgccggaac tcataatacc agatgctgct
     1501 tcaatggtta atggtattgt gaaagggacc cttccacagg tagccctaac ccttggaaat
     1561 gcagtccttg caacagcctt gctgatttca gacctgcttg atcgcaaggt taaggaaaag
     1621 gagcttcttc tcagcatgag tgcaatgtgc ctactttcta caccttttgg tggatttcca
     1681 atgtgccacg gagctggtgg ccttgtcgcc cagtataggt ttggagcaag aacgggtggg
     1741 tccaatataa tatcgggaac aattctcttg gtagttgcaa tactttttgc cacaccacat
     1801 ctggaactta tcataccatt tggagccctt ggtgcaatgt tgttctattc agcaattgcc
     1861 ctttacagga cagcaacaaa aaccgaggat atgagcttta cagcgattac aggtgtaatc
     1921 gcattcttcc tgggtatggc atgggcgttt gtaatcatgc ttctggccca taatattcaa
     1981 aagagaataa agggagtaga ttctcaaaaa tagtggtata taatcgagat agatacaatt
     2041 tgatgcatca aggaggacaa taatgaagtt tttaaatgtt agtaaggaaa atatggatat
     2101 agatgggatt ccagctattg tcctcaggcc agtattggaa atgggctctt atcctactgt
     2161 ggtattttac cacggctgga gctcaaaaaa ggagtaccag gaatttaggg gtgctgtcat
     2221 tgcaagcttg ggctaccagg tgataattcc agatgccctg catcacggtg agagagggga
     2281 attggattat gctgcaactg aaaacgccgg ctttttctgg agggttgttc taacaaacct
//
LOCUS       DASRQF010000310         2657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_64990, whole genome shotgun sequence.
ACCESSION   DASRQF010000310 DASRQF010000000
VERSION     DASRQF010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64990"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            370..1095
                     /locus_tag="VFD79_08290"
     CDS             370..1095
                     /locus_tag="VFD79_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZH94091.1"
                     /translation="MTNYNEFMSEFNRIEDFLKDLVNAKYNMPFYKLLEEAMKRDKLV
                     KQFYQELRTMSDLRNIIAHGDPNDPVALPSKSTLNRIRFIEDQFTNPLKIIDVFKKSV
                     DAFDATDSLEEVLSEIDKYHYSQFPVVKSGEFIGLITENGITNWLASSVKNDNISIKN
                     TTVQDVILGDEESDSYSFMLTSDTLYDVIDKFEEIRRKKNRTATIIVLNRKVNPVNVE
                     DIYTILTPWDLDIIFSKLGMGLE"
     gene            1301..2395
                     /locus_tag="VFD79_08295"
     CDS             1301..2395
                     /locus_tag="VFD79_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HZH94092.1"
                     /translation="MRNLMRTSLIVVFALLFTVSVSFASTNFSDVDNHWGQTYIDYLA
                     SQGLISGFPDGTFKPNETLTRAQVSSILSSELNLNPEPASFPDVSASHWANGHIGAVV
                     SQGIMNGYPDGSFRPDNPMTRAEVSAVIAAAYNMTQSTSNSPFSDLAQNHWSFSNVMA
                     LLDSYVISGYPDGTFRPNNSMTRAEFSVVMGKAINPLFTQEAALTSDALDIAQILKDE
                     DMAALAGYAHPVDGIRFSPYYYIENSHQVFTAAQIPSLLADNSVYFWGIQDGSGFDID
                     ETPQDYFDRYVNARDFTSPDETVYNNVIARGNLINNIPSFYPNAVFVELHVEGTQQYS
                     GMDWRSLYLIFEEYNGDFYLIGIANGEWTT"
BASE COUNT          835 a          469 c          571 g          782 t
ORIGIN      
        1 agtgttaaaa agcctgtgtc cctctattta caagggacac aggctctatt ttttgtgttt
       61 tggcgctaaa ctcaaggtat aaaccatatt agggttttta ccaagacaaa tttatgttaa
      121 tatcaaaagc aaagtttacc actatgacgg tgaattagtt taccgggtac atggagtatt
      181 aaaaggtttt atttgagcaa atgatttcag tgtgactgta gccgatacaa tggacccgta
      241 tcgccgtgat ggtgggtctg ttttaccgtt acagtggagt aagtgtaccc aaatactcaa
      301 cttagggtaa ctatattaca gataatccaa gtggataagg tttaaatgaa attttcaagg
      361 aggagttaga tgacaaatta caatgagttt atgagtgaat ttaacagaat agaggatttt
      421 cttaaggacc tggtaaatgc aaaatacaac atgcccttct ataagctatt ggaggaggcc
      481 atgaaaaggg acaagctggt taagcagttt tatcaggagt taagaaccat gtctgacttg
      541 cgtaacataa ttgcccatgg agaccctaat gaccctgtag cactgccttc aaagtcaact
      601 cttaatagga taagattcat tgaggatcaa tttaccaatc cactaaagat tattgatgtt
      661 tttaagaaaa gcgtagatgc ttttgatgcc actgacagcc tggaagaggt cttgagcgag
      721 attgataaat accattattc gcaatttcca gttgtgaagt ctggggagtt cattggcctt
      781 attacagaga atggaattac aaattggctg gcctcgagtg tgaagaatga taacatctcc
      841 ataaaaaata caaccgtaca ggatgtgatc cttggggatg aggaaagtga cagctacagt
      901 tttatgctta cttcagacac actgtatgat gtcattgata aatttgagga aataagaagg
      961 aagaaaaaca gaacggcgac aatcattgtt ttgaaccgaa aagtgaatcc agttaatgtt
     1021 gaggatatct acacaatatt aactccttgg gatttggata taatttttag taaattggga
     1081 atgggcttgg agtaattaca tcattctctt gatatgcaat taacttgttc atagaggaga
     1141 tttaatcagc aatagaattg gctacttaat atatatggta cttaaaagca cccctggaat
     1201 gttttgattc cagggatttc tgtgtttgtt tacacattga tatggttctg tcacgttttt
     1261 tagggtatta cataactaaa gtattcatgg aggtgaatga atgagaaatc taatgagaac
     1321 atctttaatt gtagtctttg cattactatt tacagtttct gtctcatttg caagtacaaa
     1381 tttttccgat gtagataacc actggggaca gacctacatt gattatctgg catctcaggg
     1441 cttaatcagt ggttttccag atggcacttt caaaccaaat gagacattaa caagagcaca
     1501 ggtcagttca atactttcaa gcgaattaaa ccttaatcct gagcccgcaa gctttccgga
     1561 tgtgtcggct tcacattggg caaatggtca tattggtgct gtagtatccc aagggataat
     1621 gaacggctat ccggatggta gcttcagacc agacaaccct atgacaagag ctgaggtttc
     1681 tgcagttatc gctgcagctt acaatatgac ccaaagcact tcaaatagtc catttagcga
     1741 cttagcacaa aatcactggt cattttcaaa tgttatggct cttttagaca gctatgtaat
     1801 ctctggttac ccagacggta ccttcaggcc aaacaattca atgaccagag ctgaattttc
     1861 agtggtcatg ggcaaggcaa taaatccgct gttcacccag gaggcggctc taacctctga
     1921 tgctctagac atagcccaaa ttcttaagga tgaagatatg gctgctcttg caggctatgc
     1981 tcatccagtt gatggcataa ggttttctcc atattactat atagagaaca gccatcaagt
     2041 cttcactgct gctcaaatcc caagtctgct tgccgataac agtgtatatt tctggggtat
     2101 tcaggatggg tcaggttttg acatcgacga gacccctcaa gactactttg acaggtatgt
     2161 aaacgccagg gactttacgt ctcctgatga gaccgtatac aacaatgtca tagccagggg
     2221 aaatctaata aacaacattc cttccttcta cccaaatgca gtatttgtgg agcttcatgt
     2281 ggaagggacc caacaataca gcggaatgga ctggagaagt ctctatctga tatttgagga
     2341 gtacaacgga gacttttacc ttattggtat cgcaaatgga gaatggacaa cttagatcag
     2401 ggacagaaca tgcaaagaca gcattgagat gacaaatacc aagaaagatc gaggtacacc
     2461 ccaaatgtta gactaaaatc taatgtttgg aggtgtattt tttattgctt gatttgggat
     2521 ttattgtttg agaatatgga agatgatata ctgattatag ctaaggttta tgaccaattc
     2581 tccttaaaaa gcttaggcaa agggagcgga ttaatatctt gttcctgtag gatgacagaa
     2641 aacaaagaaa tcagagg
//
LOCUS       DASRQF010000311         5394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1303113, whole genome shotgun sequence.
ACCESSION   DASRQF010000311 DASRQF010000000
VERSION     DASRQF010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303113"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="VFD79_08300"
     CDS             <1..757
                     /locus_tag="VFD79_08300"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HZH94093.1"
                     /translation="GNMESLLYSFLAGSSTAIGAILLMIFGKPSRKMMALLLGFAAGI
                     MVAVSVFELMPEAYQFSNSISVVAFGFILGCGMMYLLDVMLPHAHMSSSDELVVENEA
                     HIHEEKKVLRMGYLIFFGIAIHNVPEGLAIGAGLEASPELGLTIALAIGLHNIPEGLA
                     VAGPLKAGGLTNLKVVLFTMGAGLMTVVGTAIGLVVFGISSAFVGGSLAFAAGAMIYI
                     VNDELIPQANNLHSHLANAGSIAGMLIGFAMLI"
     gene            921..2372
                     /gene="guaB"
                     /locus_tag="VFD79_08305"
     CDS             921..2372
                     /gene="guaB"
                     /locus_tag="VFD79_08305"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004838182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HZH94094.1"
                     /translation="MEFVGEGLTFDDVLLLPGKSEVLPRNTNISTQLTRKIKLNIPLM
                     TAGMDTVTESRMAIAIAREGGIGIIHKNMSIEKQALEVDRVKRSEHGIITDPFSLSKD
                     HAVSDALELMERYRISGVPIVEEDNTLVGILTNRDIRFEKDITRKIDEVMTKENLVTA
                     SIDITMDEALEIMKQHKFEKLPLVDEDNKLCGLITIKDIEKSIEFPHSARDEKDRLLA
                     GAAVGVTADMMERVAALVKSKVDVVVVDTAHGHSKGVLEAVSMIKANYPDLQVIAGNV
                     ATAEATVDLIKAGADAVKVGIGPGSICTTRVVTGIGVPQVTAVMNCARAAAEFEVPVI
                     ADGGIKYSGDITKAIAAGANVVMAGSMFAGTEESPGEEVLFEGRRYKEYRGMGSMGAM
                     DSGSKDRYFQEDTKKYVPEGVEGRVHYKGKLGDVVYQLVGGLKSGMGYVGAKDIPELQ
                     EKAKMIKITGASLVENHPHHITITKESPNYQRG"
     gene            2701..>5394
                     /locus_tag="VFD79_08310"
     CDS             2701..>5394
                     /locus_tag="VFD79_08310"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HZH94095.1"
                     /translation="MKIGLDVGSTTLKCVVLDEKDNLVFHSYERHFSQIREKTIVLLN
                     EIIKRFPTEKRASLSISGSAGMGLAEKHDIPFIQEVYATRLAVMRQVEGTDVVIELGG
                     EDAKILFLSNGLEVRMNGSCAGGTGAFIDQMATLMGISLTDMNELSKSHEKQYTIASR
                     CGVFAKSDIQPLLNQGASKSDMSASIFQAVANQTIGGLAQGRPIKGKVVYLGGPLTFF
                     SQLRETFNRALGVNGICPENSLYFVAMGAALSDFETPVNLDDLVKRLDKHQSTKSYGG
                     LKRLFRDEEDYMVFKSRHELEQVTYSDPFTYTGDAYLGIDAGSTTIKAVIMDGEDRIL
                     HSIYRSNQGNPVPLIRDFLLDFYKKYPNINVKSSAVTGYGEELLKNAFSLDYGVVETI
                     AHYTAAQKYKPEVDFIIDIGGQDIKCFKIKDGVIDSIFLNEACSSGCGSFLQTFAEAL
                     GYDIDQFANLGLYSTDPVDLGSRCTVFMNSSVKQAQKDGASMADISAGLSISVIKNAL
                     YKVIRASSAKDLGENIVVQGGTFYNDTVLKAFEDELGQKVIRPNIAGLMGAYGAALYA
                     RDMGGEGSSLLNEDQLGMFNHQVRVVMCGLCENNCSLTVNSFGDNRKYIGGNRCDRPL
                     KNRNTDDRYDMYEEKLKLLKEYKSIKGRRGSIGIPMALNMYEMLPFWHTVFTLLDFEV
                     IRSPFSSRELFIRGQHTIPSDTVCFPAKLVHGHIEYLIDRDVDTIFYPCLTYNIDEKM
                     GDNHFNCPVVAYYPEVIAANIERLEGTNFIFDYMGLKNKKVLSKKLFEIMQRTHKDIK
                     LNEIKFAVDRGIKEYGTYIKKVQAKGQEFLLKAREEGRRIIVLAGRPYHVDPETNHGI
                     AKLITEYGVVVLSEDSISNLASRTPVDILDQWTYHSRLYTVA"
BASE COUNT         1615 a         1024 c         1368 g         1387 t
ORIGIN      
        1 aggaaacatg gagagtttac tttatagttt tttggcaggt agctcaactg caattggggc
       61 aatattactt atgatatttg gaaagccaag caggaaaatg atggccttgc tgctgggatt
      121 tgctgcagga ataatggtgg cggtctctgt atttgaactt atgccagaag catatcagtt
      181 cagcaactca atatctgttg tagcatttgg atttatacta gggtgtggta tgatgtacct
      241 tcttgacgtt atgcttcctc atgcccacat gtcctcctca gatgaactgg ttgtggaaaa
      301 tgaagcccat attcatgagg aaaaaaaggt gttgagaatg gggtatctta tattttttgg
      361 tatagccatc cacaatgtac cagaaggact tgcaattgga gcgggattag aggcaagtcc
      421 cgaacttgga ttgaccatag ccttggctat tgggctgcat aacattcctg aaggattggc
      481 tgttgcaggg cctctgaagg ctggtggcct aacaaatcta aaagttgtat tgttcacaat
      541 gggggcagga ctgatgactg ttgtaggaac agcaattgga cttgtggtgt ttggaatatc
      601 atctgcattc gtaggtggat cccttgcatt tgctgcaggt gccatgatat acatagtcaa
      661 cgacgaactg atacctcagg caaacaattt acacagtcat cttgccaatg ccggctcaat
      721 agctggaatg ctcatagggt ttgccatgct tatatgatag gaatccggca agatgagaac
      781 caaagaccac acagcagctt agaatatttt aggctgctgt atttttttaa aagctaccct
      841 ttttctttcc ttgacattac ccatggcttt tgttacatta taaagaaata taaactgggt
      901 ttgaagaagg aggagttaat ttggaatttg tgggagaggg tttaaccttt gatgatgttc
      961 ttttattacc tggaaaatca gaggttctgc caagaaacac caatataagt acacagctta
     1021 ccagaaagat aaagcttaat attccattga tgacagcagg aatggataca gtgactgaat
     1081 caaggatggc cattgccatt gctagagagg gcggaatagg tattatccac aagaacatgt
     1141 ccattgaaaa gcaggctctt gaagtggaca gggtcaaaag gtcagagcat gggattatta
     1201 ctgatccatt ttcactttct aaggaccatg cggtttcaga tgcattggaa ctgatggaaa
     1261 ggtacaggat ttctggagtt ccaattgttg aagaggacaa tacccttgtg gggatactca
     1321 caaacaggga cattaggttc gagaaggaca taacgagaaa aatagacgag gttatgacca
     1381 aggaaaacct tgttactgca agtatagaca taaccatgga tgaggccctt gaaataatga
     1441 agcagcataa gttcgagaag ctgccattgg tggatgagga taacaagtta tgtggtctga
     1501 taaccatcaa ggacatagag aaatcaatcg agtttccaca ctcagcaagg gatgaaaagg
     1561 acaggctcct tgcaggtgca gccgtaggag tgactgctga tatgatggaa agggttgccg
     1621 cccttgttaa gagcaaggtg gatgtggttg ttgtggatac tgcccatggt cattcaaagg
     1681 gagtccttga ggctgtttca atgataaagg caaactatcc ggaccttcag gtcattgcgg
     1741 gaaatgtcgc cactgcagaa gcaacagtgg accttatcaa ggctggagcc gatgctgtca
     1801 aggttggaat tggacctgga tccatctgta caacaagggt tgttacaggt attggagtgc
     1861 cacaggttac tgcagttatg aactgtgcaa gggctgcagc tgagtttgaa gtcccagtaa
     1921 tagcagatgg agggatcaag tattcaggag atatcacaaa ggcaattgca gctggagcaa
     1981 atgttgtaat ggctggttcc atgtttgcag gaaccgaaga gagccctgga gaagaggtgc
     2041 ttttcgaggg aagaagatac aaggaataca ggggtatggg atccatggga gcaatggatt
     2101 caggcagcaa ggacaggtac ttccaggagg ataccaagaa gtatgtacct gagggagttg
     2161 aaggaagggt ccattacaag ggtaagcttg gagatgttgt ttaccagctt gtaggcggat
     2221 tgaaatcagg tatgggctat gtgggagcaa aggatatccc agagcttcag gaaaaggcaa
     2281 agatgatcaa gatcacaggg gcttcccttg ttgaaaacca cccacaccac attacaatta
     2341 caaaggaatc accaaactat cagagaggat agttttagaa accaggcgag agcctggttt
     2401 ttcaattcac aattctcagt tcacgattca caatttttag ggatcatcct tggggtttga
     2461 tttgcaaatc taaggtacta ctatatttag tccaaaaaga tatttaattg caatttctca
     2521 atacccttta caggctgcct aacagttcat atgctgcaag tcatgtccta atgtgattct
     2581 gatttttttg attctttcaa ttgtgaaatg tgaactgtga attgagaatt gtcaaatata
     2641 gacttttatc gataaatgta gtaaaatgat tgcataatct gaagaattaa ggtgatcata
     2701 atgaaaattg gcttggatgt cggttcaact actttgaaat gtgtcgtgct ggatgagaag
     2761 gataacttgg tctttcactc ctatgagcgg cacttttcgc aaatcaggga aaagacaata
     2821 gttttgctca atgaaataat caaaaggttt ccaactgaaa aaagggcaag cctttcaatc
     2881 tcaggatctg ccggaatggg acttgctgaa aagcatgaca ttccttttat ccaggaggta
     2941 tatgccacca gactggctgt aatgagacag gtggagggaa ctgatgttgt aatcgagctt
     3001 ggtggtgagg atgcgaagat tctcttccta tcaaacggct tggaggtaag gatgaacggg
     3061 tcctgcgctg gtggcaccgg agcctttata gaccagatgg caacccttat gggtataagc
     3121 ctcactgata tgaacgagct gtcaaaaagt catgaaaagc aatatacaat agcatcaaga
     3181 tgtggtgtgt ttgccaagtc ggatatacag cccctcctaa accagggagc cagtaagagc
     3241 gacatgtctg caagcatatt tcaggctgtg gcaaaccaga caatcggggg cttggcacag
     3301 ggaaggccta tcaagggaaa ggtggtttac ctgggtggac ccttgacctt cttttcccag
     3361 ctgagggaaa ccttcaacag ggctctggga gtcaatggaa tctgccctga aaactccctc
     3421 tactttgttg caatgggggc agccttaagt gatttcgaga cacctgtaaa ccttgatgac
     3481 cttgtgaaaa gacttgacaa gcaccagtca acaaaatcat atggtggact gaagagactc
     3541 tttagggatg aagaagacta catggtgttt aagagcaggc atgaattgga gcaggtaacc
     3601 tattcagatc cctttacata cacaggtgat gcctatcttg ggatcgatgc tggctccact
     3661 accatcaagg ctgtaataat ggatggggag gacaggatcc tccacagcat atacaggtcc
     3721 aaccagggga atccagtacc cttgataagg gactttctgt tggacttcta caagaagtat
     3781 ccaaacataa atgtcaaatc atcagctgta acaggctatg gcgaagagtt gctgaaaaat
     3841 gcattctcac tggactatgg tgttgttgaa accattgccc actatacagc cgcacaaaaa
     3901 tacaaaccgg aagttgattt tataatagac attggtggcc aggacatcaa gtgctttaag
     3961 ataaaggatg gggtcataga cagcattttc ctcaacgagg cctgttcatc agggtgtgga
     4021 tcatttctgc agacctttgc tgaagccctg ggatatgaca tcgaccagtt tgctaatctg
     4081 ggcttatact caacagatcc agttgacctt ggctcaaggt gtacggtctt tatgaactcc
     4141 tcagtcaagc aggcccagaa ggatggtgcc agcatggccg acatatctgc cggactctcc
     4201 ataagtgtta taaagaacgc cctgtataag gtaatcaggg cctccagtgc aaaagacctg
     4261 ggcgagaaca tagttgttca aggaggaacc ttctataatg ataccgtgct caaggcattt
     4321 gaggatgaac tggggcaaaa ggtcataagg cccaatattg ctggccttat gggagcatac
     4381 ggtgcagccc tctacgcaag ggacatggga ggtgaaggga gttccctcct taacgaagac
     4441 cagctaggga tgttcaacca ccaggtaagg gttgtaatgt gtggactgtg tgaaaacaac
     4501 tgcagcctta cggtcaacag ctttggagac aacaggaagt acattggcgg aaacaggtgt
     4561 gacagaccac ttaagaacag gaatactgat gaccgctatg atatgtacga agagaagctg
     4621 aaactcctga aggagtacaa gtcaataaag ggaagaaggg gatccatagg aataccaatg
     4681 gccctgaaca tgtacgagat gctgcccttc tggcatactg tatttaccct tctggatttt
     4741 gaggtcatac gttcaccttt ttcttcaagg gagcttttca tcaggggtca gcacaccata
     4801 ccttcagaca ctgtatgctt cccggcaaag ctggttcacg gccacataga atacctgatc
     4861 gacagggatg ttgatacaat attctatcca tgcctgacat acaacattga tgagaagatg
     4921 ggggacaacc atttcaactg tcccgtagtt gcatattatc cggaggttat tgctgcaaac
     4981 atagagagac tggagggtac aaacttcata tttgactaca tgggacttaa gaataaaaag
     5041 gtcctttcca agaaactgtt tgaaattatg caaagaactc acaaggatat aaagcttaat
     5101 gagattaaat ttgctgtgga taggggaata aaggaatatg ggacctatat caagaaggtc
     5161 caggcaaagg gtcaggaatt ccttttaaag gccagggaag agggtagaag gatcatagtt
     5221 ctggccggaa gaccctacca tgtggatcct gaaacgaacc atggcatagc gaagctgatc
     5281 accgagtatg gtgtggttgt tctaagcgag gactcaataa gcaatcttgc atccaggact
     5341 cctgtggata ttctggacca atggacctac cactcaagac tttacaccgt ggca
//
LOCUS       DASRQF010000312         2185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1806453, whole genome shotgun sequence.
ACCESSION   DASRQF010000312 DASRQF010000000
VERSION     DASRQF010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806453"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1818)
                     /gene="feoB"
                     /locus_tag="VFD79_08315"
     CDS             complement(<1..1818)
                     /gene="feoB"
                     /locus_tag="VFD79_08315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HZH94096.1"
                     /translation="MQNIALVGNPNSGKTTLFNVLTGSNQHVGNWPGVTVEKKEGSFK
                     HKGVLYNVVDLPGTYSLGAYSEDELVARDFIVKGSPDVVINVVDATNLERNLYLTTQL
                     LEMGANVVVALNMMDEAESKQIRISTKDLSEALGVPVISTVAAKKKGIEDLVDGTLKA
                     MSHEPRKVVFTYGKEIDEEIANIRNLLKEESLPYPEDWMALKLLEGDQEMVSIIEGMG
                     PVKDHLETLLSNNVDYELDIVDKRYAYVNGITKKCVVKPDDEIITTTDKIDRVLTSRL
                     FGLPIFGLIMYLVFQLTFVVGSDFLGGYVETAIEIFGEFVEGWLVSMGSPHWLVSFVI
                     EGFVGGVGAVLVFVPLIMVLYFFLAILEDTGYMARAAYIMDGIMRAVGLHGKTFISMI
                     VGFGCNVPGIMATRTLENKKDRMIAILINPFMSCGARLPIYLVFIAAFFPNNGGLVLF
                     IIYALGIGMALLMGKIFSKTLFKGESSHFIMELPPYRLPSASSVLRDMWEKVWDFVKR
                     AGTVIFAVVALLWVLSVLPMGVEPYSQESILGRVGEFIAPLFAPAGFGSWQASVSLFA
                     GIAAKEAVVAVLGMVYAGVSEGSELVTAIRESFTTLSAVS"
     gene            complement(1867..2085)
                     /locus_tag="VFD79_08320"
     CDS             complement(1867..2085)
                     /locus_tag="VFD79_08320"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HZH94097.1"
                     /translation="MVLSGVKKNDKCRIKQVMGGGYARKRLYELGLNSGAELKMVKND
                     FGPIIISMSGSKLAIGRGLASHILVERD"
BASE COUNT          609 a          558 c          426 g          592 t
ORIGIN      
        1 tgatactgca gacagagttg taaaggattc cctgattgcc gtaaccagct cgctaccttc
       61 actgacacct gcatagacca tccctaggac tgcaacaacc gcctccttgg cagctattcc
      121 ggcaaagagg cttacggatg cctgccaact accaaagcct gctggtgcga ataggggggc
      181 aatgaactct ccaaccctgc ccaggatact ctcctgggag tagggttcaa cccccatggg
      241 cagaacggat aggacccaca ggagtgcaac cactgcaaat ataaccgtac ctgccctttt
      301 gacaaagtcc cataccttct cccacatgtc ccttaggaca ctggatgctg agggtagtct
      361 gtagggtggc aactccatga tgaagtggga ggattctccc ttgaataggg ttttgctgaa
      421 aatcttgccc atcagcagag ccattcctat tcccagtgca tagattataa atagaactag
      481 tccgccgttg tttgggaaaa atgcagctat gaacacaagg taaatcggaa gtctggctcc
      541 acaggacatg aaggggttga taagtattgc aatcatcctg tccttcttgt tttccagggt
      601 tcttgtggcc attattccag gtacattaca gccaaatccc acaatcattg atatgaaggt
      661 ctttccatga aggcctacag cccgcattat tccatccatt atataggcag cccttgccat
      721 gtagcccgta tcttcaagta ttgcaaggaa gaagtagagg accatgatca ggggaacaaa
      781 gaccagtact gcaccaactc ctccaacaaa accctctata acaaatgaga ccaaccaatg
      841 gggagatccc attgaaacca gccagccctc cacaaactct ccgaaaatct caattgccgt
      901 ttcaacatag cctcccagaa aatcagaccc aaccacaaag gtaagctgga agacaagata
      961 cattatcaga ccaaatatgg gaaggccaaa gagcctgctt gtaaggaccc tgtctatctt
     1021 gtcggtggta gtaatgattt catcatccgg cttaacaaca cacttctttg taataccatt
     1081 tacatatgca taccttttgt ccactatatc aagctcgtaa tccacattgt tggaaagtag
     1141 ggtttccagg tggtctttaa caggacccat tccctcaata atggaaacca tttcctggtc
     1201 tccctcaaga agcttcaatg ccatccagtc ctccggatag ggaagggatt cctccttgag
     1261 caggttacgg atatttgcaa tttcttcatc gatctccttg ccataggtaa aaacaacctt
     1321 ccttggctca tgggacatgg ccttgagggt tccatcaaca aggtcctcaa tacctttttt
     1381 cttggcggca acggttgaaa tcacaggaac tccaagtgct tctgaaaggt cttttgtgga
     1441 tatcctgatc tgtttggact cagcctcatc catcatgtta agggccacaa caacattagc
     1501 acccatttcg agaagttggg ttgtcaggta gaggtttctc tcaagatttg tggcatcaac
     1561 tacgtttatt acaacatccg ggcttccctt aacaatgaag tcccttgcaa ccagctcatc
     1621 ctctgaataa gccccaagac tatatgtacc aggaaggtcc actacattat aaaggactcc
     1681 cttgtgctta aaggagcctt cttttttttc cacagtcact cccggccagt ttccaacatg
     1741 ctggtttgag ccggtaagca cattaaacaa ggtggttttc ccgctgttgg gatttcccac
     1801 caatgctata ttttgcataa attgtgtcct cctcgttgaa acctattatc aagcaaatcc
     1861 aaaaagctag tccctctcca caaggatatg ggaagcaaga cctctaccta ttgcaagttt
     1921 agagcctgac atggaaatta ttattggacc gaaatcattc ttaaccatct ttaactcggc
     1981 tccgctgtta agaccaagtt catatagtct tttcctggca tagcctccac ccataacctg
     2041 cttgattctg cacttgtcat tcttttttac tcctgaaagt accatagtgc acttcaacac
     2101 ctttctatgt aaaataatga aatatcttga taactattat caattataga ataatacatt
     2161 tccgacggaa ttgcaaggtt ttttt
//
LOCUS       DASRQF010000313         4874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_69069, whole genome shotgun sequence.
ACCESSION   DASRQF010000313 DASRQF010000000
VERSION     DASRQF010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69069"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..817
                     /locus_tag="VFD79_08325"
     CDS             128..817
                     /locus_tag="VFD79_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HZH94098.1"
                     /translation="MNLKHRYDRNKSLISQEDQDLLASKTIAVIGAGGLGGHLAELIA
                     RLGIGKLIIIDGDVFEESNLNRQRFSNENNLGKPKALEAKRELEKINSQVDIIAVHDT
                     VSKKNGKDLLEGADLVLDGVDNARTRLLLEDLCNQLQIPLVHGAIGGWYGQVSVVMPG
                     DWTLSTIYSDPDMEGVEKELGNPSFTPALIASYQVAEALKVVLGIGEILRKKILFIDT
                     LTNNTNVIEIE"
     gene            934..2835
                     /locus_tag="VFD79_08330"
     CDS             934..2835
                     /locus_tag="VFD79_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HZH94099.1"
                     /translation="MREDLCYVISKDNHNKEDLFKILEDNPQIRFVSLGGVDWIGHET
                     EEKIPVKVFRDDIELFLHGIAVQTDGSSVYLPEIATLDNAKIDMKADIEANWFVDYNY
                     ENIHQESGKPVGTLKIPCFLYHDGIPVDSRHILKDATRYFESHLEELLRENPDQTRAY
                     GFSPEEIKKVHVTAATELEFWVRTPDSNRELQELTTSQELNEQYWAKTRGAVRTALEN
                     SLQYMELYGMEPEMGHKEVGGVKARLQHDGSFSGIMEQLEIDWKYSTTMQTGDNEMFI
                     KHIIKETFRAKGMDVTFLAKPIPGVAGSGEHTHMGVAVELESGKRINLFHPYRKHFMS
                     PIGYGAIMGILNNYEVMNPFITSSNESFKRLKPGFEAPVCIVTSLGRSVDIPSRNRTI
                     LIGLIRDENNPLATRFELRSPNPHTNSFLCMSSMLMAMDDGIDHVLKEKRDEDSLLEE
                     LSKAPEDEGIYLQKGRAYRSEVNVFEDYTDAERDRIFGKVPATVYENIKAFQVYPEKL
                     KILKRGRVFRDSVIDSYQSAVLNKWFTEIEHRIIPDLMREIRGYKLLHDPANASDLDI
                     SNWLAIRDLRVELMKDSLNRTSLFTQIKEALDKKNFDLASSLQKDIYTKMDNLRMLYN
                     LYKKNLLDI"
     gene            3070..4773
                     /locus_tag="VFD79_08335"
     CDS             3070..4773
                     /locus_tag="VFD79_08335"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HZH94100.1"
                     /translation="MKKLLVLLLAFAMALGMAACSSTPAEEPAGELTTLTGIGEGFGG
                     PIEVEVTMDGDTITAVEVVSHSETPGISDPAIEETPAAIVEANSTEVDVVSGATYTSE
                     GIIYAVNNAIDPAAYPAPVEEEPEEAGEVIASDVYLGFGIANMGRKGPGADNEGVQVW
                     SINQVLASVLFDGEGRILEMYVDQLEIATPNYDGDGMPHFSGFPGQGGYNWDENHDEV
                     IDGKTPDTEENFFAEIDGWMTKRERGDSYVMGNGKTWVQHMDGFQKAFAGLTVEEVKD
                     WFDNYTSDRNGRPLKDGSEDADDKAKYDALTDDEKAMLADVTSAATMSLNDSHGDIIA
                     AIELAYENRKPLEIEEAATYGLGMANMGRKGPGADNEGVQVWSINQIVANSLFDNDGR
                     LVALHVDQLEIATPNYDGEGMPHFSGFPGQGGYNWDENHDEVIDGKTPDTEENFFAEI
                     DGWMTKRARGDSYVMGNGKTWVQHMDKFEEAFIGMTIDELEEWFGKYTSDRNGRPLKD
                     GSDDAEDKAKYDALTDDEKAMLADVTSAATMSLNDSHGDIIAAIRASFENRKDINLTI
                     E"
BASE COUNT         1586 a          965 c         1227 g         1096 t
ORIGIN      
        1 attttttcca ggagttggga tgggattttg aaaccggact gccatctgaa gacttcctaa
       61 aggaaattgg agccctggat tatcttctgg aggatcttta caggaaaaat taaaggaggg
      121 ctgattcttg aaccttaaac acagatacga tagaaacaaa tccctaatct cacaagaaga
      181 ccaagacctt cttgccagca agaccatagc ggtgattgga gcaggggggc ttggtggcca
      241 tcttgcggag ttgattgcaa ggctgggcat aggtaagctg ataatcattg atggggatgt
      301 gtttgaagaa agcaacctta acaggcagag attctccaat gaaaacaatc ttggcaaacc
      361 aaaggctctg gaagccaaga gggaactgga aaagatcaac tcccaggtgg atataattgc
      421 ggtccatgac actgtttcca aaaaaaacgg caaggacctg cttgagggtg ctgacctggt
      481 ccttgatggg gtggacaatg caaggacaag acttctcctg gaggacctgt gcaaccagct
      541 ccagataccc ttggtccacg gagcaatcgg aggctggtat ggtcaggtat cagtggtgat
      601 gccaggggat tggaccctgt ccaccatcta ttcagacccg gatatggagg gggttgagaa
      661 ggaacttgga aatccatcct tcactccagc cctcatagca tcctaccagg tggcagaggc
      721 cttgaaggtg gttctgggaa ttggcgagat tcttagaaaa aaaatactct ttatcgatac
      781 actcacaaac aacacaaatg taatagagat tgaataagct aagctggacc ccttactggg
      841 gtccttttac atgataaagc aaatttcttg acaagctcac tgtgtagtaa taaaataatg
      901 aagtaaaaaa atatgtctac cggagatgat aagatgagag aagatttgtg ctatgtaata
      961 agtaaggata accacaataa ggaggacttg ttcaagattc tggaggacaa tccccaaata
     1021 aggttcgtct cccttggtgg cgtggactgg ataggacatg agacagaaga aaagatacca
     1081 gtcaaggtct ttagggatga tattgaactt ttcctacatg gaatagctgt tcaaactgat
     1141 gggtcctcag tctaccttcc tgagattgca acactggaca atgcaaaaat agacatgaag
     1201 gcagatattg aagccaattg gtttgttgac tacaattacg agaacatcca ccaggagtct
     1261 ggaaagccag tagggacact taagattccc tgcttcctat accatgacgg aattcctgtg
     1321 gattcaagac atatactaaa ggatgccaca agatactttg aaagtcacct ggaggaactc
     1381 ctcagggaga acccagacca gacaagggcc tatggcttta gcccggagga gataaaaaag
     1441 gttcatgtaa ccgctgcaac agagctggaa ttctgggtta gaacacctga ctcaaacagg
     1501 gagctacagg aacttaccac atcacaggag ctgaatgagc agtattgggc caagaccagg
     1561 ggggccgtta ggacagcact tgaaaattca ctccaataca tggagctata cggaatggag
     1621 cctgagatgg gacacaagga ggtcggtgga gtcaaggcaa gacttcagca cgatggaagc
     1681 ttctcgggaa tcatggagca gcttgaaata gactggaaat attcaacaac catgcagaca
     1741 ggcgacaatg aaatgttcat aaagcacatc atcaaggaaa ccttcagggc aaagggaatg
     1801 gatgtgacct tccttgcaaa gccaatacct ggagttgcag gaagtgggga gcacacccat
     1861 atgggagttg ccgtggagct tgagtccggc aagaggatca acctcttcca cccctacagg
     1921 aagcatttca tgagtcccat tggatatggt gcaataatgg ggatattgaa taactatgag
     1981 gtaatgaacc ctttcattac atcctcaaac gaatccttca agagactcaa gcctggattt
     2041 gaagcacctg tttgcatagt tacatccctg ggaagaagcg ttgacatccc ttccaggaac
     2101 aggaccatcc tgatcggtct gatccgggat gagaacaacc ctctcgcaac aagatttgag
     2161 ctgagaagtc caaacccaca tacaaactcc ttcctgtgca tgagctcaat gcttatggct
     2221 atggatgatg gcatagacca cgtacttaag gagaaaaggg atgaggacag tcttctggag
     2281 gagctgtcaa aagctccgga ggatgaagga atctatctgc aaaagggtag ggcctacagg
     2341 agtgaggtaa atgtctttga ggactataca gatgcggaaa gggataggat attcggaaag
     2401 gtgcctgcaa ctgtttatga gaacatcaag gcattccaag tctatcctga gaagctgaaa
     2461 atcctaaaaa gaggtagggt ctttagggac agtgtaattg acagctacca gtcggcggtt
     2521 ctcaacaaat ggttcactga gatagagcac aggatcatac cggacctgat gagggagatt
     2581 agaggctaca agctccttca cgaccctgcc aatgcatcgg acttggacat atccaactgg
     2641 cttgcaatca gggacctaag ggttgagcta atgaaggaca gccttaacag gacatccttg
     2701 ttcacccaga taaaggaagc tcttgacaag aaaaactttg acctagcatc ttccctccag
     2761 aaggatatct atacaaagat ggacaatctg aggatgctct acaacctgta caagaaaaac
     2821 ctattggaca tttaaggcca ccgccactgg atacccagcg gcggttccat ttttaacaaa
     2881 atccattaaa acaatggata tgttttacat atagaagcat tgaactctgg atattcgcaa
     2941 taaacatatg acaataatgt tgaaataaca acgtttttaa aataaaatac attgaataca
     3001 ttaccacaat atgatatatt tgacaataag ataacgttaa atttaaaaca ataaaaggag
     3061 atgagtcatt tgaaaaagct gttggtatta cttttggcat ttgctatggc attaggcatg
     3121 gcagcatgtt catcaacacc agctgaggag cctgcagggg agctgacaac acttacaggt
     3181 ataggtgaag gtttcggtgg acccattgag gttgaagtaa caatggatgg agatacaata
     3241 actgcagtgg aagttgtttc acatagtgaa actcctggaa taagcgatcc cgcaatcgag
     3301 gagaccccag ctgcaatagt ggaagctaat tcaactgagg ttgacgtggt ttctggagct
     3361 acatacacaa gcgaaggaat aatctacgct gtaaacaatg caattgatcc cgctgcatat
     3421 ccggcacctg ttgaagaaga gcctgaagag gcaggagagg ttatcgcttc agatgtctat
     3481 cttggatttg gaatagcaaa catgggaaga aaaggtcctg gagcagacaa tgaaggagta
     3541 caagtatgga gtataaacca ggtacttgcc agcgttctgt ttgatggcga gggaagaatc
     3601 cttgaaatgt atgttgacca gctggagatt gcaactccaa actatgatgg agatggaatg
     3661 ccacacttca gcggatttcc aggccaggga ggctacaact gggatgagaa ccacgacgag
     3721 gtcatagatg gcaagactcc tgatacagag gagaacttct ttgcagaaat cgatggatgg
     3781 atgacaaaga gggaaagagg cgactcctat gtaatgggca atggtaaaac atgggtccag
     3841 catatggatg gcttccagaa agcctttgct ggccttacag ttgaagaggt caaggactgg
     3901 tttgacaact acacatccga cagaaacgga agaccactca aggacggatc agaggatgca
     3961 gatgacaagg caaaatacga tgcccttact gatgatgaga aggcaatgct tgcagatgta
     4021 acatcagctg caacaatgag tcttaacgac tcacacggcg atataatagc agcaattgaa
     4081 ctggcatacg aaaacagaaa accacttgaa atagaagagg ctgcaacata cggacttgga
     4141 atggcaaaca tgggcagaaa gggacctgga gccgacaacg aaggagttca ggtatggagc
     4201 atcaatcaga tagtggcaaa ttcactgttt gacaatgatg gaagacttgt cgcacttcac
     4261 gttgaccagc ttgagattgc aactccaaac tacgatggag aaggtatgcc acacttcagc
     4321 ggattcccag gccagggagg ctacaactgg gatgagaacc acgatgaggt catagatggt
     4381 aagactcccg atacagagga gaacttcttt gcagaaatcg atggatggat gacaaagagg
     4441 gcaagagggg actcctatgt aatgggtaac ggtaaaacat gggtccagca catggataag
     4501 tttgaagagg catttattgg aatgactata gatgagcttg aagagtggtt tggaaagtac
     4561 acttccgaca gaaacggaag accacttaag gatggatcag atgatgcaga ggacaaggct
     4621 aaatacgatg cacttactga tgacgagaag gcaatgcttg cagatgttac ttcagctgct
     4681 acaatgagcc ttaacgattc acacggtgac ataatagctg caataagggc atcatttgaa
     4741 aataggaagg acatcaacct gacaatagag taacttcccc aatcttgaaa accctggatc
     4801 ttgtttccag ggtttttcaa aaaacacaaa aagaaccggt ccctttgtgt tttttgaaag
     4861 aatgaaaaat aacc
//
LOCUS       DASRQF010000314         2395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_502387, whole genome shotgun sequence.
ACCESSION   DASRQF010000314 DASRQF010000000
VERSION     DASRQF010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502387"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VFD79_08340"
     CDS             <1..294
                     /locus_tag="VFD79_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH94101.1"
                     /translation="SISWQSDKLDIPKLSVELDGELILEGDLETYFDRVLEKYPPEAR
                     RPVEAGIEDMSLLMESEEIRLLVVFESIEIGVDTTNNRKNHWFMLAGMYVMEK"
     gene            complement(360..590)
                     /locus_tag="VFD79_08345"
     CDS             complement(360..590)
                     /locus_tag="VFD79_08345"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZH94102.1"
                     /translation="MKKQKRSNKRGSGRVKKARIIESKCDRSPFCPVKRVCPTGAVER
                     AGFFMGKVLINDEKCIGCGRCIQVCPHNAVFF"
     gene            773..2284
                     /locus_tag="VFD79_08350"
     CDS             773..2284
                     /locus_tag="VFD79_08350"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004839131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HZH94103.1"
                     /translation="MKDNDKKITSKNNVKGKEPGITTAAGSPVTNNQDSMTVGPRGPL
                     VLQDLWFMEKMAHFDREVIPERRMHAKGSGAFGVFTVTHDITKYTKAAMFSEVGKKTE
                     MLVRFSTVAGERGAADAERDIRGFAMKYYTEEGNWDLVGNNTPVFFFRDPLKFPDLNH
                     AVKRDPRTNLRSPNTNWDFWSSLPEALHQVTITMSDRGIPLSYRHMHGFGSHTYSMIN
                     AQNEKVWVKFHFHSQQGIKNLTDQEAGELVGKDRESHGRDLYEAIEKGEFPRWTMFIQ
                     VMTLEEAENMPYNPFDLTKVWYKGDFPLIEVGYFELNRNPDNYFAEIEQAAFNPANFV
                     PGISYSPDKMLQGRLFSYGDAQRYRLGVNHHQIPVNSPKNAKNVHSYHRDGMMRVDGN
                     LGSELHYEPNSYGNWTEYKDHELPEEKADGNISRWNFREDDDDYYTQPGKLFNLMNDE
                     QKQVLFENTARNMGDSEEMIKLRHIKNCFHADPEYGKGVAKAMGMEIDFTDWM"
BASE COUNT          779 a          437 c          566 g          613 t
ORIGIN      
        1 tcgatatcct ggcaatcaga taaattagat atccctaagt tatcagttga gctggatggt
       61 gaactgatac ttgagggtga cctggagacc tactttgata gggttcttga aaagtatccg
      121 ccagaggcca gaaggcctgt ggaggcgggc attgaggata tgagccttct tatggagtcg
      181 gaggaaatca ggttactggt ggtgtttgag agtattgaaa ttggtgtgga tacaaccaac
      241 aacagaaaaa accattggtt tatgcttgca ggaatgtatg taatggaaaa gtgaaacaac
      301 acagcttaat ctaagtcaat tgaaggctca ccccaaggtg agccttcact ataagtaaat
      361 caaaaaaata ctgcattatg tggacatacc tggatacatc ttccgcaacc tatgcatttt
      421 tcatcgttta ttaaaacctt acccatgaaa aaaccagccc tttcaactgc acctgtcggg
      481 cagaccctct ttacaggaca gaaaggagac ctgtcacatt tggattcaat tattctagct
      541 ttcttgactc ttccgctgcc tctcttattg cttctttttt gttttttcaa tttgcacctc
      601 cctattgtag ttcaatctaa ttatacaccc atagggggta tatgtaaaga atgtcccgaa
      661 tccagattca taaagttgat ataatgaagg tttcagtttc aattcaacag ggtaatatag
      721 taaagaacaa ttgttgatag tttgataacc catgtattga aagggggata atatgaaaga
      781 caatgataag aaaataacca gcaaaaataa tgtaaagggg aaggagcccg gaataaccac
      841 agctgcaggt tcaccagtga caaacaatca ggattcaatg actgtgggac caagaggtcc
      901 acttgtcctt caggatcttt ggtttatgga aaaaatggca cacttcgaca gggaggtaat
      961 tcctgaaaga aggatgcatg ccaagggatc aggagctttt ggagtattta cagtgaccca
     1021 tgatattact aaatacacta aggctgccat gttttcggaa gtaggaaaga agactgaaat
     1081 gctggtaagg ttttcaaccg ttgcaggaga aagaggagct gctgatgcag aaagggatat
     1141 cagaggcttt gcaatgaagt attatactga ggaaggaaac tgggacttgg taggaaataa
     1201 cacacctgta tttttcttta gggatccgct taaatttcct gacttgaatc atgccgtcaa
     1261 gagagaccca agaaccaatc tgagaagtcc aaacaccaac tgggatttct ggtcatcatt
     1321 gccagaggca ctacaccagg tgacaataac aatgagtgac aggggaattc cattatccta
     1381 cagacacatg cacggatttg gatcacatac atactcaatg ataaatgcac agaatgagaa
     1441 agtatgggta aaattccatt ttcattcaca gcagggaata aagaacttaa ctgatcagga
     1501 ggctggagaa ctggtaggaa aggacaggga aagccacggg agagaccttt atgaggcaat
     1561 tgaaaaaggt gaatttccca gatggaccat gttcattcag gtcatgaccc tggaagaggc
     1621 tgagaacatg ccgtacaacc catttgacct gacaaaggtc tggtataagg gcgattttcc
     1681 actgatagag gttggttatt ttgaacttaa cagaaatccg gacaactatt tcgctgaaat
     1741 agagcaggct gcattcaatc cagcaaactt tgtacctgga atatcatact caccggacaa
     1801 gatgctgcag ggtagactct tctcctatgg agatgcccaa aggtatagac ttggtgtaaa
     1861 ccaccatcag atacctgtga actctcctaa gaatgcaaag aatgttcatt cataccatag
     1921 ggatgggatg atgagagttg atggaaacct tggctcagaa ctccactatg agcctaacag
     1981 ctatggcaac tggactgaat acaaagacca tgaacttcca gaggagaagg ctgatgggaa
     2041 catatcccgt tggaatttcc gtgaagatga tgacgattac tacacccagc ctggaaagct
     2101 gtttaacctg atgaatgatg aacagaaaca ggtccttttt gaaaatacag ctagaaacat
     2161 gggggattct gaagagatga taaagctaag acacataaag aactgtttcc atgctgatcc
     2221 cgagtatggc aagggagttg ccaaggctat gggaatggag atagatttta cagactggat
     2281 gtaggattat gatgaatgtt gcaagcaaaa tgcaatgggc tgaagggtcc attgcatttt
     2341 gacgagaatt gaggtgtcca tatgaaagtg aaattaaaat tcattggggt ggtgg
//
LOCUS       DASRQF010000315         3430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_190715, whole genome shotgun sequence.
ACCESSION   DASRQF010000315 DASRQF010000000
VERSION     DASRQF010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190715"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2839
                     /locus_tag="VFD79_08355"
     CDS             <1..2839
                     /locus_tag="VFD79_08355"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HZH94104.1"
                     /translation="RKEDARVHDVLLCVQTGKTIVDEDRMKFPTEEFYLKSKEEMEGL
                     FPDHLQALENTQDIADRCNVTMDFETLHLPEYKVPEGYSNESYLRDLCERGLVERYGE
                     VTEEIRNRFEYELSVILQMGYTDYFLIVWDFIKFARDNQIMVGPGRGSAAGSVVSYAL
                     NIIDIDPLEYGLLFERFLNPERVTMPDIDIDFCYERREEVIQYVIGKYGADRVAQIAT
                     FGTMAARGAIRDVGRAINMPYGEVDYIAKQIPTELGITIDRALEISKTLKTEYDENPD
                     VRNLIDLARAVEGLPRHTSTHAAGVVISKEPVTKYAPLLRNNDAITTQFTMIELEDLG
                     LLKMDFLGLRTLTVIRDAVDLIKENHGRDIDFSTITFDDPKVLDLFAKAETLGIFQFE
                     SPGMRAFLKELKANVFEDLIAANSLFRPGPMNQIPTFVECKHDPTQISYLHPTLEPIL
                     DVTYGCIVYQEQVMEIVRKLGGYSFGRADLVRRAMGKKKMDVMEKERKNFIYGQTDQD
                     ENVILSGAIRNGVDEKTASQIFDLMIDFANYAFNKSHSAAYAVIAYRTAWLKVYYPVE
                     FMAALISSIMGNTSQVSLYIQECKRLQIDVLPPDVNHSQGKFTVENRSIRYGLSAVKN
                     VGENFIRAIVKARGDGKFRGFTDFIERIEKTDSSSMNKRAVESLIKAGAMDSLGPNRA
                     QLLSIFEKTIDGIHSDRKRNIEGQFSLFQSDESVVRDRLPDLREFPQNTLLQMEKEML
                     GIYVSGHPLMPYEKEVKLLSNFSTADLITQGLDDSIHPSGVRDGQRVAIAGIIVEKKN
                     KVTKNNNMMAFISLEDLYGTIECIIFPAIYEKNYKLIEEDRIVLLEGRLAISEVEDPK
                     IIADRLSPITKINLGKIYIKLSGMADKSSLDDIKEILARYPGDIPVYVYFEKERRTVA
                     ADRSLWIDGSQDGLILELQDRLGAGSVKISN"
     gene            2875..>3430
                     /locus_tag="VFD79_08360"
     CDS             2875..>3430
                     /locus_tag="VFD79_08360"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002840236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HZH94105.1"
                     /translation="MKTIGVLTSGGDAPGMNAAIRAVIRTGIYNKIRMLGIREGYQGL
                     LEGDLDDMDTASVGDIIQRGGTILRTDRSEEFMTEAGFRKALNVIDVYNMDGLIVLGG
                     DGTFKGAQELSQAGVPTIGIPCTIDNDLAYTDYTIGFISAVDTAVDAIGHIRDTSTSH
                     GRSVVVEVMGRHCGDIALYAGLAGG"
BASE COUNT          998 a          722 c          920 g          790 t
ORIGIN      
        1 tcgcaaggag gatgcaaggg tccacgacgt tctcctttgt gttcaaaccg ggaagacgat
       61 agtcgacgag gacaggatga agtttcccac ggaggagttt tacctaaagt caaaagagga
      121 gatggagggc ctgtttccag accacctcca ggctcttgag aacacccagg acattgccga
      181 caggtgtaat gtgacaatgg actttgaaac cctgcacctg cctgaataca aggtaccgga
      241 gggttattcc aacgaatctt accttaggga cctttgtgaa aggggacttg tggaaaggta
      301 tggagaggtt acagaggaaa ttaggaacag gtttgagtac gaattgagtg taattctcca
      361 gatgggctat acggactact tcctcatcgt ctgggacttc atcaaatttg caagggacaa
      421 tcagataatg gttggacctg gtagggggtc tgctgctgga agtgttgttt cctacgcact
      481 gaacatcata gacatcgatc ccctggagta tgggctgctc tttgagaggt tccttaatcc
      541 tgaaagggtg acaatgccgg acatcgacat agacttctgc tatgagaggc gggaggaagt
      601 gatacagtat gttatcggaa agtatggtgc tgacagggtc gcccagattg ccaccttcgg
      661 taccatggct gcaaggggtg ccatcaggga tgttggcagg gctataaaca tgccctatgg
      721 cgaggtggac tacattgcca agcagattcc aacagagctt ggcataacca tagacagggc
      781 ccttgaaata agcaaaaccc tgaagacaga gtacgacgag aatccagatg ttagaaatct
      841 catagatctt gcaagggctg tggagggctt gcccaggcat acatccaccc atgctgcagg
      901 ggttgtaatc tccaaggagc ctgttacaaa atatgccccc ctgttgagaa acaacgatgc
      961 aataaccacc cagttcacaa tgattgagct tgaagacctg ggacttttaa agatggactt
     1021 tcttggactt agaaccttga ctgtcattag ggatgctgtg gaccttataa aggaaaacca
     1081 cggcagggat attgatttta gcacaatcac ctttgacgat cccaaggttc tcgacctttt
     1141 tgccaaggct gaaaccctgg ggatcttcca gtttgaaagt ccgggaatga gggccttcct
     1201 gaaggagctc aaagccaatg tatttgagga ccttattgct gccaactctc tgttcagacc
     1261 aggacccatg aaccagatac caacctttgt tgaatgcaag catgacccga cgcagatttc
     1321 atacctccac cccacactgg agccgattct ggatgttacc tacggctgca tagtctatca
     1381 ggagcaggtt atggaaatcg taaggaagct gggaggctac tcctttggtc gggctgacct
     1441 tgtacgaagg gctatgggta agaaaaagat ggatgttatg gaaaaggagc gcaagaactt
     1501 catttatggt cagacagacc aggatgagaa tgtaatcctt tcaggggcaa ttagaaacgg
     1561 ggtggatgaa aagaccgcat cccagatatt tgaccttatg atagatttcg ccaattacgc
     1621 cttcaacaag tcccactctg cagcctatgc ggttatagcc tacaggacgg cgtggctgaa
     1681 ggtatactat ccagtggagt ttatggctgc cctgatttca tccattatgg gcaacacctc
     1741 ccaggtatca ctctacatcc aggaatgcaa gagacttcag atagatgtac ttcctcctga
     1801 tgtaaaccac tcacagggaa agttcaccgt tgagaatagg agcatcagat atggtctttc
     1861 agcggttaag aatgtgggag aaaacttcat aagagctatt gtaaaagcca gaggagacgg
     1921 caaattcagg ggcttcacag attttatcga aagaattgaa aagaccgaca gcagctcaat
     1981 gaacaaaagg gcagttgaga gcctgataaa ggctggagcc atggattcac tgggaccaaa
     2041 cagggcacag ctattgtcca ttttcgaaaa gaccatagat ggtatccact ccgacaggaa
     2101 acggaatatc gaggggcagt tctccctctt ccagtcagat gaatcggttg tcagggacag
     2161 gctgccggac ttgagggagt tcccgcagaa tacacttctg caaatggaaa aggaaatgtt
     2221 gggtatatat gtctcagggc atcctttaat gccctatgaa aaggaggtca agctattgtc
     2281 aaacttcagc acggctgacc ttataaccca gggattggat gattcaatcc acccttcagg
     2341 ggtcagggac ggacaaaggg ttgccatcgc cggcattatt gttgagaaga agaacaaggt
     2401 taccaagaac aacaatatga tggcctttat aagccttgaa gacctttacg gaacaataga
     2461 gtgcataata tttcctgcca tatatgaaaa gaactacaag cttattgagg aggacaggat
     2521 tgtgctcctg gagggacgcc ttgccatttc tgaggttgaa gaccccaaaa tcatagctga
     2581 caggctatcg ccaatcacta agataaattt gggaaagata tacattaagc tttcgggaat
     2641 ggctgacaag agtagccttg acgacatcaa ggaaatactg gcaagatatc cgggggatat
     2701 tcccgtgtat gtgtattttg agaaggagag aaggactgtt gccgctgacc gaagcttgtg
     2761 gattgacgga agtcaggatg gcctcatcct ggagctgcag gataggcttg gtgctggtag
     2821 cgtaaagata tctaactagt tgaagtaact atactaattg cggaggggat aaggatgaag
     2881 accataggag tacttacaag cgggggagac gcaccgggga tgaacgctgc aataagggct
     2941 gtaataagaa ccggaatcta caacaaaata aggatgcttg gaataaggga aggctaccag
     3001 ggcctccttg aaggtgacct ggacgatatg gataccgcat cagttgggga cattatccaa
     3061 agaggcggaa ccattctgag aactgacagg tcagaggaat tcatgaccga ggctggcttc
     3121 agaaaggcct tgaatgtaat agatgtatac aacatggacg gtttgatagt cctgggtgga
     3181 gacggtacct tcaaaggagc tcaggagctg agccaggcag gtgtaccaac aattggaatc
     3241 ccctgcacaa tcgacaacga ccttgcctac acagactaca ccattgggtt catttcggca
     3301 gtggatacgg cagtggatgc aatcggacac atcagagaca cttcaacttc ccacggaaga
     3361 agtgtggttg tagaggtgat gggtcgacat tgtggagata tagcactcta tgcaggcctt
     3421 gccggaggcg
//
LOCUS       DASRQF010000316         7447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_207412, whole genome shotgun sequence.
ACCESSION   DASRQF010000316 DASRQF010000000
VERSION     DASRQF010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 7447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7447
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207412"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VFD79_08365"
     CDS             complement(<1..349)
                     /locus_tag="VFD79_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH94106.1"
                     /translation="MKFKKTIVTLGVAGLLLGSTAVFSEPGSESDPLVSLSYLNTKLE
                     EIRDYIDEKLDDRTEVPTSGNALVVVELQSGQVLEGEAGTEIIKRSGEAVAIASSLGG
                     LSDVTAGADIADGG"
     gene            613..1782
                     /locus_tag="VFD79_08370"
     CDS             613..1782
                     /locus_tag="VFD79_08370"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZH94107.1"
                     /translation="MKELSRVIQNYPASGIRKMFELADKYDNVISLTVGEPAFDTPEN
                     IKDAAKLSLDKNNTHYVSNAGIPQLRMAVAEKYNRLVGDYYTPDNVMVAVGGMEAIFL
                     ALLATVNPGDEVLIPDPGYPNYQGQTVILGAKAVKVPIYEKNNFNIQAEDIEAAITEK
                     TKVLILNSPSNPLGSVLSKDELAEIAAVVKKYDLFVISDEVYEMIIYDGHEHHSISQF
                     PEVRENVLIVNSLSKTYAMTGWRIGYVVGSEKIIQAMPKIQEGIASCVSHFIQDAAVE
                     AIQNGQDSVEFMQREYSRKRDILIEEINNIRGLDYKGSPGSFYAFVNIKSTGLSSQEF
                     AERLVVQEQVLVVPGSAFGSMGEGYVRMVFAGSDEDLREAFKRIKRFVEGLGNTP"
     gene            complement(1828..2850)
                     /locus_tag="VFD79_08375"
     CDS             complement(1828..2850)
                     /locus_tag="VFD79_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004820540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HZH94108.1"
                     /translation="MKKVVTLGEIMLRLSTPGFNRFVQSSSFDVNYGGGEANVCVSLA
                     NYGLEARFVTRVPDNEIGQSAINSLRRYGVVTDHIAIGGERLGIYFLETGASQRPSKV
                     IYDRGYSSISQADPSDFDWERIFSGADWFHFTGITPALGDKAAALTLEACRAASEMGL
                     TVSVDLNYRKKLWTSQKANEVMSGLMKYVDICIGNEEDAERVFGIKASGTDVDSGKID
                     HLAYEEVAKQLMDRFGFKFVASTLRESHSASHNGWSATIYDGHKSYLSRRYEVDIVDR
                     VGGGDSFASGLIYGMITGKPIQEALEFAVAASALKHTVPGDFNHVSVGEVLALMGGDG
                     SGRVQR"
     gene            complement(2864..3511)
                     /locus_tag="VFD79_08380"
     CDS             complement(2864..3511)
                     /locus_tag="VFD79_08380"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HZH94109.1"
                     /translation="MIQKINTLTRIWEAGIVAVIRADKCETAEKIARACIAGGIPAIE
                     ITFTVPGAARVIEELRASIGREDLIIGAGTVLDSETARLAILAGAEYIVSPGFHEETA
                     RLCNRYGIPYMPGCMTTTEIIRAMEAGADVIKIFPGSMVGPEYIKAIKGPLPQASLMP
                     TGGVSLHNVDQWIRNGCIAVGVGGELTAGAKTGDYEKVTGAAIEFVKRIKAARMK"
     gene            complement(3786..4133)
                     /locus_tag="VFD79_08385"
     CDS             complement(3786..4133)
                     /locus_tag="VFD79_08385"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002835111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HZH94110.1"
                     /translation="MIVKRGDIYYADLSPVIGSEQGGVRPILVVQNDVGNKYSPTIII
                     AAITSQINKARLPTHVEITAQDFGLPKDSVVLLEQIRTIDKKRLREKIGKFDEDMMRK
                     VDQALKISVGLGN"
     gene            complement(4130..4363)
                     /locus_tag="VFD79_08390"
     CDS             complement(4130..4363)
                     /locus_tag="VFD79_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH94111.1"
                     /translation="MSERDECLPMVIEDSLELFDKVVAMCNRSEFHCKKYREFMRGYL
                     EVNHLNITLAEMGLEEDITDLMGYESSLEYEKI"
     gene            complement(4456..5631)
                     /gene="alr"
                     /locus_tag="VFD79_08395"
     CDS             complement(4456..5631)
                     /gene="alr"
                     /locus_tag="VFD79_08395"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HZH94112.1"
                     /translation="MDKFYDVRPTYVEINLDNLSHNFREIQKIVKTGTIVMPVIKANG
                     YGHGSIELGRMYKELGAERVAVSLLNEGIELRRAGLDLPILVLNYTPGGQMKEVAAYD
                     LTQAIYRYEDAKALSEAAGEMGKTAKIHIKIDSGMGRLGFLPNEESVEEILRLKDLEN
                     IEIEGIFTHFAKADELDKTVTRDQYRKYSWTLEELEKKGFSPPIKHVSNSAAIIDLPE
                     YNLDMVRPGIMLYGYYPSEEVDKSRIELRPAMTLKAVVSNIKTVPEGTGISYGHLYEA
                     PRESRIATVPIGYADGYTRMLTGKAEAHVGGGLAPIAGKICMDQLMLDVTDIPNVEIG
                     DQVVFFGEGLEDYPTVDQVAEKLGTINYEIVCMMGRRLPRVYKKDGKIIKTIDYLLV"
     gene            complement(5694..6938)
                     /locus_tag="VFD79_08400"
     CDS             complement(5694..6938)
                     /locus_tag="VFD79_08400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HZH94113.1"
                     /translation="MKTIYFKDDFMGIKTGVDIVSNRRIEKLMESKRDSFYRRLFCDS
                     EIKYLESKNHAVGTVAGLYAAKEAVSKVLGTGIGKIGWKDMWIMHEDGGRPYLGYGEK
                     MKTFMDLEGISHIDISISNEEEYSMSIAVGEANKKYQIPDNMEFRLPARPLDTHKGDF
                     GRVGVIGGSQGMLGAVYLATYGALRSGAGLVHAILEPDLARDFGLKVIEVMVREATDI
                     YAYRKAQEGLSSLVLGPGYGLGHKQKANIEDCLLNFENPIVLDADGLNNLSDNPTIIR
                     HRKGKTIITPHPGEMGRLLKMSTAEVQNDRESAAREFANVYGVVTVLKGHNTIVTDGE
                     RIYVNRSGNPGMATAGSGDILSGVAGTFLAQVDDPFQAAVMAVYVHGLAGDMAKEEKG
                     EYGMIASDIIEYLPRAVDLIVE"
BASE COUNT         1922 a         1839 c         1484 g         2202 t
ORIGIN      
        1 tgccaccatc tgcaatatca gctccagcag tcacatctga taggccacct agacttgagg
       61 caatggcaac agcctcgccg ctccttttga taatctcggt tccagcttcc ccctcaagga
      121 cctgtccgct ttgaagttcc acaacaacaa gcgcatttcc agatgttggg acttcagttc
      181 tgtcgtcaag cttctcatct atgtagtccc tgatttcctc aagcttagta ttcagatagc
      241 ttaaagacac aagaggatct gattcacttc ctggctcaga aaatacagca gtagatccaa
      301 gcaatagtcc tgcaactccc agagtcacaa tagtcttttt aaatttcata tgaacactcc
      361 tcgcattatt tggattgggt tttcgtttca tagttagcca agttacatta tactataagt
      421 gggcggtaaa tgtcacccac atctgcctat tgtgtggttt ttgtatggaa taattctact
      481 ttcaccgggc atatctttca ccactgatgg gtataagtcc agttgaaggt ttgtgtacat
      541 atataaaccg attggtagtt gagagggtgc ggattgggag atgattttca agttgaaatg
      601 ggagggattc tattgaagga gttatcaagg gtaatacaga actaccctgc atcaggaatc
      661 agaaagatgt ttgagctggc ggacaagtac gacaatgtaa tcagccttac ggttggagaa
      721 cctgcatttg acacccctga aaacatcaag gatgcagcta aattgtcact ggacaaaaat
      781 aatacccact atgtttccaa tgctggtatt ccccagctaa ggatggccgt tgcagaaaag
      841 tacaacaggt tggttggtga ttattacacc ccagataatg ttatggttgc agttggaggt
      901 atggaagcaa tattccttgc cttgttggca actgtgaatc ccggtgatga ggtcttgata
      961 ccagatcccg gatatcccaa ttaccagggg cagaccgtca ttcttggggc taaggctgtg
     1021 aaggttccaa tctatgaaaa aaacaacttc aatattcagg ctgaggatat tgaggctgca
     1081 ataactgaaa agaccaaggt cttgatactc aattcaccaa gcaatcccct gggctctgtc
     1141 ctgtcaaagg atgagctggc agagattgca gcagtggtta aaaagtacga cctgtttgtg
     1201 atttctgatg aagtctacga aatgatcata tacgatggac atgaacacca cagcatatcc
     1261 cagtttccag aagtcagaga aaatgtcctt atagtaaata gcttgtcgaa aacctatgcc
     1321 atgacagggt ggagaatagg ctatgtcgtg ggcagtgaaa aaatcatcca ggcaatgcct
     1381 aaaatccagg aaggaatagc atcctgtgtc tcccatttca tccaggatgc tgcagttgaa
     1441 gccatccaga atggacagga ctcagtggaa ttcatgcaga gggagtactc caggaaaagg
     1501 gatatcctaa tagaggaaat caataatatt aggggacttg actacaaggg gtctcccgga
     1561 agtttctatg catttgtcaa cattaagagc acgggactga gctcacagga atttgcagag
     1621 aggctggttg ttcaggaaca ggtgctggtg gttccaggct cagccttcgg gtccatggga
     1681 gagggatatg tgaggatggt ttttgcaggg tctgatgaag acctgagaga agcctttaaa
     1741 cggattaaaa ggtttgtgga gggattggga aacaccccct aggctaaaag gacgggagca
     1801 gaattctgct cccgtcgatt atttccatta tctttggaca cggcctgacc cgtcccctcc
     1861 cataagtgca agaacctccc ctacactcac atggttgaag tctcctggaa cagtatgctt
     1921 cagggccgat gcagccactg caaactccaa ggcctcctgg ataggcttgc ccgtaatcat
     1981 cccgtaaatg aggccactgg caaaggaatc gccccctccc accctgtcga caatatctac
     2041 ctcatacctt cttgaaagat atgatttatg gccgtcatag atggttgcgg accaaccgtt
     2101 atgggatgcg gaatgacttt cccttagtgt cgatgccaca aacttgaatc caaacctgtc
     2161 cattagctgc ttggctacct cttcataggc caaatggtcg atcttccctg aatcaacatc
     2221 tgttccactg gccttgattc caaataccct ttctgcatcc tcctcattcc ctatgcagat
     2281 atctacatat ttcataagtc ctgacattac ctcatttgcc ttttgggatg tccacagctt
     2341 ctttctgtag ttaaggtcca ctgacactgt caatcccatt tcactggcag ccctgcaggc
     2401 ttcaagggtc aatgctgcag ccttgtcacc aagggcagga gtaattcctg tgaagtggaa
     2461 ccagtcagcc ccactgaaga tcctttccca gtcaaagtct gatgggtcag cctgtgaaat
     2521 cgaagaatac cctctgtcat atataacctt tgaaggtctc tgggaggcac cggtctcaag
     2581 gaagtatatt cccagccttt cgcctccaat ggcaatatgg tcggtgacaa ccccgtacct
     2641 tctcaaggag ttgattgcac tctgacctat ttcattgtca ggaacccttg tcacaaacct
     2701 ggcttccaga ccatagttgg ccagggacac acagacattc gcctctccac caccatagtt
     2761 tacatcgaag ctgcttgatt gaacaaacct gttgaagcca ggtgtggaaa gcctaagcat
     2821 aatttccccc aaggtaacaa cctttttcat atctgatctc cccctatttc attctagctg
     2881 ccttgatcct tttgacaaac tcaattgcag ccccagttac tttttcataa tctccggttt
     2941 tcgcacctgc tgtaagctct ccccctactc caacagcaat gcaaccattt ctgatccact
     3001 ggtcaacatt atggaggctc actcctccgg ttggcatcaa ggaagcctgt ggcagggggc
     3061 ccttgatggc ttttatgtat tctggtccta ccatgctgcc tggaaaaatc tttattacat
     3121 ctgctcctgc ttccattgct cttatgattt cagtggttgt catgcagcct ggcatgtatg
     3181 gaattccgta ccggttgcaa agccttgcag tttcttcatg gaagcccggg cttacaatat
     3241 actcagctcc tgcaagtatg gccaatcttg cagtctcgct gtcaaggacg gtgccggctc
     3301 cgattatgag gtcctccctc ccaatggaag ctctcaattc ctctattacc ctggctgcac
     3361 caggaaccgt aaatgtaatt tcgatagccg ggattcctcc agctatgcat gccctggcaa
     3421 tcttttctgc tgtttcacat ttatcagccc ttatgacagc tacaattcca gcctcccaga
     3481 tcctggtcag ggtattgatc ttttgaatca tccaaatcac ctacctattt tatttttgat
     3541 acttgtccca tttatcctta ttttgattct atcaagctat gatgcattaa tcaattgaaa
     3601 caatccaaat aggttgctgt tttgcccttg aatgtataaa aagcagctcc ggaataagga
     3661 aactccgaag ctgcttttta tacatgctgg tatccatatg taggtgaatt gaatcactca
     3721 attcatgtgg acaaagccgt ccacattgat tctcagccat gaacttaata tgtaggagga
     3781 ttgtcctagt ttccaaggcc tacacttatt ttaagtgcct ggtcgacctt tctcatcata
     3841 tcctcatcaa acttaccaat tttttctcta agtcttttct tgtcaatggt cctaatctgc
     3901 tcaaggagca ccactgaatc ctttggaaga ccgaaatcct gtgctgttat ttcaacatgg
     3961 gtcgggagtc tggccttgtt gatctgagag gtgatggcgg caattatgat tgtgggactg
     4021 tacttgttac ccacatcatt ctgtactacc agaatcggcc gtacaccacc ctgctcagaa
     4081 ccaataacag gactcaagtc tgcataatat atgtctcctc tcttcactat catatttttt
     4141 catactccag tgaagattca tatcccatca ggtcggttat atcttcctca agacccattt
     4201 cagcgagggt tatgtttaaa tggtttacct cgagataccc tctcataaac tctcggtatt
     4261 ttttgcagtg gaactcactg cgattgcaca tggcaaccac cttgtcaaaa agctccaagc
     4321 tatcttctat aaccataggg agacattcgt ctctttcact cattcggtta accccccctt
     4381 tcatgtatac ttagagaaat atcagtatat tttaattata tcccgatatg aaaaaccctg
     4441 tcaatgcttc tttgactaaa ccagtagata atcaattgtc ttgattatct tcccgtcctt
     4501 cttgtatacc cttggaagtc tcctgcccat catgcatact atttcgtagt tgattgtccc
     4561 cagcttttca gccacctggt ctacggttgg ataatcttca agtccctctc caaaaaaaac
     4621 cacctggtca ccaatttcca cattgggaat atcggtcaca tccagcataa gttggtccat
     4681 acagatttta cctgcaattg gagcaagtcc accgcctaca tgggcttcag cctttcctgt
     4741 aagcatcctg gtataaccgt ctgcatatcc tatggggaca gttgcgattc tagattccct
     4801 tggtgcctca taaagatgcc catagctgat acccgtacct tcagggactg tcttgatgtt
     4861 tgagaccaca gccttaagtg tcattgcagg cctaagttca atccttgact tatccacttc
     4921 ctctgatggg tagtagccat atagcattat gcctggtctc accatatcaa ggttgtactc
     4981 aggaaggtca attattgctg ccgaatttga aacgtgtttg atgggaggac taaagccctt
     5041 cttctcaagc tcctccaggg tccaggaata cttcctgtac tggtcccttg tgacggtctt
     5101 gtccaattca tcagccttgg caaagtgggt gaaaattccc tcaatctcaa tgttttcaag
     5161 atccttgagt ctaagaatct cctccactga ctcctcattg ggcaagaaac ccagtctgcc
     5221 cattccactg tctatcttta tgtgaatctt tgccgtctta cccatctcac ctgctgcttc
     5281 tgaaagggct tttgcatctt catacctgta tatagcctgg gtcaaatcat aggctgcaac
     5341 ctctttcatc tggccgccag gagtgtagtt aagtacaaga atgggaaggt caagcccagc
     5401 tcttcttagc tcaatcccct catttaacag ggatacagcc actctttcag cacccagttc
     5461 cttgtacatc ctacctagct caatggatcc atggccatag ccatttgcct tgattacagg
     5521 cattactatt gtgcctgtct ttactatctt ctgaatttcc ctaaagttgt gtgaaaggtt
     5581 atccaggttt atctccacat atgtgggtct tacatcatag aacttatcca tggggaacct
     5641 ccttgtgtta ttatgcattt ctttggctta accgattatt ctatccttgc agtttactca
     5701 acaataagat ccactgccct gggaaggtat tcgattatgt cacttgctat cattccgtat
     5761 tcgccctttt cctccttggc catatctccg gcaagtccgt gaacatagac tgccattaca
     5821 gcggcttgga atggatcatc cacctgggca aggaatgtgc ctgccacacc tgaaagtata
     5881 tctccacttc ctgctgttgc cattccagga ttccctgacc tgttgacata tatcctttcc
     5941 ccatcggtta ctattgtatt gtgccccttt aggactgtaa caactccata tacatttgca
     6001 aattcccttg cagcgctttc acggtcattt tggacctcag cagttgacat tttcaagagt
     6061 cttcccattt ctcccggatg aggcgttatt attgtctttc ctttcctgtg ccttattatg
     6121 gtgggattat ctgacagatt gttaagtccg tctgcatcca aaacgattgg gttctcaaag
     6181 ttcagcagac aatcctcaat atttgccttc tgcttgtgtc caagaccata gcctggtcca
     6241 agaacaagtg agctcaggcc ttcctgggcc tttctgtagg cgtaaatatc ggtggcttcc
     6301 cttaccatta cctcaattac cttcagaccg aagtctcttg caaggtcagg ttcaaggatg
     6361 gcatgcacaa ggccagcccc acttctcaag gctccatagg ttgccagata tactgctccc
     6421 agcattccct gacttcctcc tattactccg actcttccaa agtctccctt atgggtgtcc
     6481 aggggtcttg caggcagcct gaattccatg ttatcaggga tctgatattt tttgttcgct
     6541 tctcctactg caattgacat actgtattcc tcctcatttg aaattgatat atcaatgtga
     6601 ctgattcctt ccaaatccat gaaagttttc attttttcac catagccaag gtagggtcta
     6661 cccccatcct catgcattat ccacatatcc ttccatccaa ttttgccgat gccggttccc
     6721 aaaacctttg atacagcttc ctttgctgca taaaggcctg caacggtacc taccgcatgg
     6781 tttttggact ccagatattt gatttcagaa tcacagaaga gcctcctgta gaagctgtcc
     6841 ctcttggatt ccataagttt ttcaattctc ctgttggaca caatgtccac acctgtcttg
     6901 atacccataa aatcatcctt aaaatagatt gttttcatct cctataataa cacaatcatc
     6961 atacaattca tacccactcg atgagcccct atgaaatcat agatttcaag gaactctatc
     7021 gtcgggcacg tggcagttca cagttggaag gatctagtta atatctaaaa tctaagattt
     7081 aagagttgaa aagctattga aaagtttctt catttaactc aataaggtga ttactttaag
     7141 cccgaaaggt gctttgctaa gaagttcata tgaaagaacg actttaaaat ccatcgagga
     7201 tttcggagtg ttttgattcc tccaagcgta gcggcatagg aatcaacgga gaaaacgagc
     7261 gcttgattat gctcgggctt gaattcagaa ctgcgtgtaa acaccagagg tcttagtaca
     7321 aacaaagtaa aattttgata ataaattatc aaaattgaaa aaatccgctt gacataatag
     7381 ataagcttcg atattataat ggtagatata tcttcagggc gaggtgcaag tcctcaccgg
     7441 cggtaaa
//
LOCUS       DASRQF010000317         5610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_813698, whole genome shotgun sequence.
ACCESSION   DASRQF010000317 DASRQF010000000
VERSION     DASRQF010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813698"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1010
                     /locus_tag="VFD79_08405"
     CDS             63..1010
                     /locus_tag="VFD79_08405"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HZH94114.1"
                     /translation="MNLCLGGIKVFTGNANRDLVKNICQELGITMGTCDVTHFSDGEI
                     NVNIGETVRGCDVFIIQPTNTPVNDNLMELLILIDAVKRASAGRVNAVIPYYGYARQD
                     RKTKAREPITAKLVANLLTTAGADRVVGMDLHAGQIQGYFDIPVDHLSAVPILAEYFR
                     EIVDEDTVIVSPDLGGVTRARQFANILDLPIAIIEKRRPKANVSEVMNVIGDIEGKNV
                     ILVDDIIDTAGTIVKAASVLKSFGAKKVYGCATHGVLSGPALDRIHNSELEKFVITDT
                     IPLSEMARIEKIEVVSVAPILAEAIKRINSNESVSIIFD"
     gene            1034..1612
                     /gene="pth"
                     /locus_tag="VFD79_08410"
     CDS             1034..1612
                     /gene="pth"
                     /locus_tag="VFD79_08410"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004813308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HZH94115.1"
                     /translation="MIVIAGLGNPGSKYSGTRHNVGFDVIDRLAEKNNIKVNRLRFKA
                     LTGEGLIGNEKVLLVKPSTYMNNSGESLREVMDFYKLEPEKLLVVVDDIDIDFATIKL
                     KKKGSAGSHNGLKSIIYLIQKDNFPRLKVGIGKKPPYYDLADFVLSKFSSDERKLMDR
                     AIEKASEAAEEFVRSGVDKAMNEFNIRNTAQD"
     gene            1646..5170
                     /gene="mfd"
                     /locus_tag="VFD79_08415"
     CDS             1646..5170
                     /gene="mfd"
                     /locus_tag="VFD79_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HZH94116.1"
                     /translation="MKDYLLDPLLNLKPYNTLLEDIKKNLSPISTHGLMEENMGHFLC
                     GLKSHTKRQILFVTYSEARARQIFEDVKAILPDQDVRHYPSKDTILYDVDAFSSERTH
                     QRLEVLSCLMENRNLIVVASVEALSDRIISKTSFQNHRLNISLGKEASLKDLSQGLVS
                     AGYERVSLVEQTGQYSIRGGILDVFPSNMEYPVRVEFFDTEIDSIRTFDPKDQRSVDV
                     IENVEIFPVAEIILPTGERDKIAAALEKDLDHFRKRSEDGEKTSKSEEKFGKYLDALH
                     EGSHITNKDLLVPYLPKDLVGTLIDFFQDQALIMLDEPKRLREKYEAYMDEFNGRTLD
                     LFQVGEVLKSHLQLKYHYEDMILEIRKRIIITSASFLKQDSDFPPKGIIKFTMRTIPV
                     YHGKLDMLKEDLDRYLYKGYKVVIMAGDQDKGKRLKDVLREIDINSELQFDRDNAIQT
                     GQVFISEAGLKAGFEYEEMKLAVITEKEIYGAGKKKAKKKSRKIKDGGQKLQFSDLNE
                     GDFIVHENYGIGQYLGVSQLSVQGIKKDYLLIQYKGKDRLYLPIEQMSMIQKYIGSDS
                     IKPKINKLSGTEWAKTKSRTKKAIEEMATDLLQLYAKRERVEGYQFSPDTPWQREFED
                     MFPYEETQGQKTSIKDIKGDMERKRPMDRLLCGDVGYGKTEVALRAAFKAVMDGKQVA
                     ILVPTTILAQQHYNTIMERLGSFPMSVAMLSRFRTRKEQQASLEMLRKGMLDIVVGTH
                     RLLSKDVVFKDLGLLIVDEEQRFGVRHKEKLKKLKENVDVLTLTATPIPRTLHMSLVN
                     IRDMSVIEEPPEERYPIQTYVTEYNRTLIRDAIQREISRGGQVYYVYNRVETIDKVAM
                     ELRNLVPEGEFAIAHGQMSERELEQVMLDFMNKEINVLVCTTIIETGLDIPNVNTIII
                     HDADKFGLSQLYQLRGRVGRSNRLAFAYFVYQKDKVLSEVAEKRLRAIKEFTEFGSGF
                     KIAMRDLEIRGAGNLLGVEQHGNIESIGYDLYVKYLGQAVRRLKGDVVQEKLDTTVDI
                     KVDGYISGRYIPDEDQKLEIYKRISTIESMDDYRELIDELIDRFGDLPKPVENLMNIS
                     YIRGLASRNHITNIVQIHQKVRVDYEAVDHIKLELIQMLTEKYGPAVSYDLSNQPSIT
                     IHAKGDVINRLKEILETIDNFAQLGNSV"
     gene            5212..>5610
                     /locus_tag="VFD79_08420"
     CDS             5212..>5610
                     /locus_tag="VFD79_08420"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HZH94117.1"
                     /translation="MKRTIRFLLIAIMAAMVLSGCSSEKVGIVAQVDGKEITQEEFDK
                     DFQVYKGLYERQLGEGALDQEGIDGKTMGESLEESILEKLIMETLILEDNEAKGIEVT
                     EEDLQEYIEDYKESIGGEESYQQFLQSNSLS"
BASE COUNT         1790 a         1106 c         1446 g         1268 t
ORIGIN      
        1 caaggctgga tgggttgaaa agaagggact aaaaaaacac aaataactag gaggatgtag
       61 agatgaacct ttgtttaggt ggaatcaagg tcttcaccgg aaatgccaat cgagacctgg
      121 taaaaaacat ttgccaggag cttggcataa ccatgggtac gtgtgatgtt acccacttca
      181 gtgacggtga gatcaatgta aatatcgggg agacagtaag gggctgtgac gtgttcataa
      241 tccagcctac caatacacca gtaaatgaca accttatgga gctgcttatt cttatagatg
      301 cagtaaaaag agcttcagca ggaagggtaa atgcagtgat accatattac ggctatgcca
      361 gacaggacag gaagaccaag gcaagggaac cgatcactgc aaagctggtg gccaatctgc
      421 tgacaactgc aggagctgac agggttgtgg gtatggacct gcatgctggt cagatccagg
      481 gatactttga cattccagtg gaccaccttt cagcagttcc aatacttgca gagtatttca
      541 gggagattgt tgacgaggac actgtaattg tttcgcctga ccttggtgga gtaaccaggg
      601 caagacagtt tgccaacatc cttgaccttc caatagccat catcgaaaag agaaggccaa
      661 aagctaacgt ctcagaagta atgaatgtta ttggtgacat cgaaggcaag aatgtaatcc
      721 ttgtggatga cataatcgac acggcaggca caatagtaaa ggctgccagt gttctgaaga
      781 gctttggagc aaagaaggtc tatggctgtg caacccacgg cgtcctatca ggacctgctc
      841 tggacagaat ccacaattcc gagctggaga agtttgtaat aacggacacg attcccttgt
      901 cagaaatggc caggattgaa aagatagagg tagttagcgt tgctccgatt cttgcagagg
      961 caataaaaag aatcaacagc aacgaatcag tcagtattat ctttgattag tcacatagca
     1021 gggagttgat tatgtgattg tgattgcagg attgggaaac cccggaagca agtattccgg
     1081 aacaagacac aatgttggat ttgatgtaat agacaggttg gctgaaaaga acaatatcaa
     1141 ggtcaacaga ttgagattca aagccttgac aggcgaaggc ctaatcggga atgagaaggt
     1201 acttcttgta aagcccagca catacatgaa caatagcggt gagtcactaa gggaagtaat
     1261 ggatttctat aagctggagc cggaaaaact cctggtggtt gtcgatgaca tagacattga
     1321 ttttgcaacc atcaagctta aaaaaaaggg tagcgcaggc tcacacaatg ggctcaagtc
     1381 aataatctac cttatccaga aagacaactt tccaaggttg aaggtgggaa tcggtaagaa
     1441 acccccatac tacgacctgg ctgactttgt ccttagcaag ttcagttccg atgaacgaaa
     1501 gcttatggac agagccatag agaaggcttc agaagctgca gaagagtttg taagaagcgg
     1561 tgtcgacaag gcaatgaatg agtttaatat tagaaacaca gcccaggact aaaacctggg
     1621 cttaaacctt ttgggaggat tggcaatgaa ggactatctt ttggatccac ttttgaatct
     1681 aaaaccctat aatacattac ttgaggatat caagaagaat ttgtcaccca tttccaccca
     1741 tgggctcatg gaagagaaca tgggtcattt cctatgtgga cttaaaagcc acacaaagag
     1801 acagatcctc ttcgtaacct acagtgaagc aagggccaga caaatatttg aggacgtaaa
     1861 ggccatacta cccgaccagg atgtaaggca ctatccttca aaggacacaa tcctctatga
     1921 tgtggatgca ttcagttcgg aaagaaccca tcagaggctg gaggtcctgt catgtctcat
     1981 ggaaaacagg aacctcatag ttgtggcttc cgttgaagcc ctttcagaca ggatcatatc
     2041 aaaaacttcc ttccaaaacc ataggttgaa tatttccctc ggcaaggagg cgagcctgaa
     2101 agacctttcc caggggcttg taagtgccgg ctacgaaagg gtcagccttg ttgagcaaac
     2161 cggtcagtac agtatacgcg ggggaattct tgatgttttc ccatctaaca tggagtaccc
     2221 ggtaagggtg gaattttttg atacggagat tgattccata aggacctttg accccaagga
     2281 ccaaaggtct gtggatgtta tagaaaatgt ggagatcttt ccagtagctg aaatcatatt
     2341 gcctactgga gaaagggaca agattgccgc tgccctggag aaggatcttg accacttcag
     2401 gaaaaggtcg gaggatggtg aaaaaacctc taagtcagaa gaaaaattcg gaaagtacct
     2461 tgatgcactg catgaaggct ctcacataac aaacaaggac cttcttgttc cgtatcttcc
     2521 aaaggacctt gtaggtacct tgattgactt tttccaggat caagccctca ttatgttgga
     2581 tgagcctaaa agacttaggg aaaagtatga agcttatatg gatgagttca acgggcgtac
     2641 cctggacctg ttccaggtag gggaggtcct taagtcacac cttcagctga agtaccatta
     2701 tgaggacatg atccttgaga taaggaaaag gataatcata acaagtgcat cctttctcaa
     2761 gcaggacagt gactttccac caaagggaat aataaagttc acaatgagga caatccctgt
     2821 ttatcacgga aagctggaca tgctcaagga agacctggac aggtatcttt ataagggcta
     2881 caaggtagtt ataatggcag gagaccagga taagggcaag cggttaaagg atgtccttag
     2941 ggaaattgat attaattcag agcttcagtt tgacagggat aatgccatac agacaggcca
     3001 ggtctttatt tcagaggctg gcctgaaggc cggctttgaa tacgaggaaa tgaagcttgc
     3061 agtcataact gaaaaggaaa tctacggagc aggaaagaaa aaggccaaga agaagtcaag
     3121 gaaaataaag gatggtggcc agaagctaca gttttcagac ctcaatgagg gagacttcat
     3181 agtccatgag aactatggaa tcggacagta cctgggagta tcccagctgt ctgtccaggg
     3241 tatcaagaaa gactatcttc tcatccagta caagggaaag gacagactct atcttccaat
     3301 tgagcagatg tccatgattc agaagtacat cggatcagat tcaatcaagc caaagataaa
     3361 caagcttagc ggtacggaat gggccaagac aaagtccagg acaaagaagg caatcgagga
     3421 gatggccaca gacctgctcc agctctatgc caagagggag agagttgaag gctaccagtt
     3481 ctcacccgat acgccatggc aaagggaatt tgaggacatg ttcccctatg aggagaccca
     3541 agggcagaaa acctccatta aggacatcaa gggtgacatg gaaaggaaaa ggcccatgga
     3601 ccgcctcctt tgcggggatg tgggttacgg caagactgag gttgcattaa gggccgcatt
     3661 caaggccgta atggacggaa agcaggtggc catacttgtt ccaaccacca ttctagccca
     3721 gcagcactac aatacaatca tggagaggct gggaagcttc cccatgtccg tggccatgct
     3781 cagcaggttc agaaccagaa aggaacaaca ggcttcactt gaaatgctga gaaaggggat
     3841 gctggacata gttgtgggga cccacagact cctgtcaaag gatgttgtgt ttaaggacct
     3901 gggtcttcta attgttgatg aggagcagag gtttggtgta aggcacaagg aaaagcttaa
     3961 aaagcttaag gaaaatgtgg atgtcctgac cctgacggca acccccatac caaggaccct
     4021 ccacatgtca cttgtgaaca tcagggacat gtcagtaata gaggagccac cggaggaaag
     4081 gtatccaata cagacctatg tgacagagta taacaggaca cttatacgtg atgccataca
     4141 gcgggagata tcaaggggtg gacaggtgta ctacgtatac aacagggttg aaacaattga
     4201 caaggtggcc atggagttga gaaacctggt tcctgaaggt gaatttgcca ttgcccatgg
     4261 acagatgtca gagcgtgaac ttgagcaggt catgcttgat ttcatgaaca aggaaattaa
     4321 cgtcctggta tgtacgacca tcattgagac cggtttggac atacccaatg ttaacacaat
     4381 aatcatccac gatgccgata agtttggcct ttcacagcta taccagctga ggggcagggt
     4441 gggcaggtca aaccgacttg cttttgcata ctttgtatat caaaaggaca aggttctttc
     4501 agaggttgcc gaaaagcgcc taagggccat caaggagttc acagagtttg ggtccggctt
     4561 taagattgca atgagagacc ttgaaatcag gggagccgga aaccttcttg gggttgagca
     4621 gcatggaaac atcgaatcaa taggctacga cctgtatgtg aagtaccttg gccaggcggt
     4681 gaggagactt aagggggatg tggtccagga gaagctggac accacagtgg atataaaggt
     4741 tgatggatac atttccggta gatacatccc tgatgaagac cagaagcttg aaatatacaa
     4801 gaggatatca acaattgaat ccatggatga ctacagagag ctcattgatg agctaataga
     4861 cagatttgga gacctgccaa agcctgttga aaacctgatg aatatttcat acatcagagg
     4921 tctggcaagc agaaaccaca taactaacat agtccagatc catcagaagg taagggttga
     4981 ctatgaggct gtagaccata tcaagcttga actgattcag atgctcacgg agaagtatgg
     5041 acctgcagta tcctatgacc tttcaaacca gccaagtata acaatacacg ccaaaggtga
     5101 tgtaataaac aggttgaagg aaattcttga gaccattgac aatttcgctc aactgggtaa
     5161 tagtgtataa tgtaaattga tgtaatgata tagaaaggat gttttattta aatgaagaga
     5221 acgataagat ttttattgat agctataatg gccgcaatgg tactttcagg ctgttcaagt
     5281 gaaaaggtag gcattgtggc acaggttgac gggaaggaaa tcacccagga ggagtttgac
     5341 aaggacttcc aggtctacaa gggcctctat gagagacagc tgggagaggg agccctcgac
     5401 caggagggaa tagacggaaa gaccatgggt gaaagcctgg aggaaagcat ccttgagaag
     5461 ctgataatgg agaccttgat tcttgaggac aacgaagcca agggaataga agtaacagag
     5521 gaagaccttc aggagtatat tgaggactac aaggaatcaa tcggcgggga agaaagctat
     5581 caacagtttc tacagtcaaa cagcctttca
//
LOCUS       DASRQF010000318         6101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_194839, whole genome shotgun sequence.
ACCESSION   DASRQF010000318 DASRQF010000000
VERSION     DASRQF010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6101
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194839"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="VFD79_08425"
     CDS             <1..1020
                     /locus_tag="VFD79_08425"
                     /inference="COORDINATES: protein motif:HMM:NF013663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HZH94118.1"
                     /translation="CLPPRGEKMSTIGIIANPASGKDIRRLVSHATVVDNNEKVNIVK
                     RIILAAQGAGVTGVMVMPDTFLIGYKAQEDLKGSRELNIPIEILDMRVKGSVEDTILA
                     AKLMEENGVQCIIALGGDGTSRAVAKSITVTPLISISTGTNNVYPEMLEGTVAGIAAA
                     AVSTRTSNLGQSCRRDKRIEIYKGGKMLDIALVDCVISKQTYIGSRAIWNPEDIQRVI
                     VSRANPASIGFSTIVGVKKIVGEDDNFGASINVNTGENELVAPIAAGTMKEIRVDEPV
                     IHPLEEMFSMTMDYKGIMALDGERELPFKKGETYHFKISRDGPYRVNIKRTIESAQIN
                     GFFNR"
     gene            1040..2344
                     /locus_tag="VFD79_08430"
     CDS             1040..2344
                     /locus_tag="VFD79_08430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HZH94119.1"
                     /translation="MAKKIVMPKLGLTMKEGKLAKWYKQEGDQVQVGDKLFSIETDKI
                     TNDAEATEAGILRRIIVQDGQTVPIMEPVGIVAGADEDISSLLSEVPETSSDKTVEKV
                     QVGKKAPEPEKKIERTGKVKISPVAKKLAEEEGIDFESIQGTGPGGRIVLDDVKNAIE
                     NKGGTKASPAAEKMAKEKGIDISGISSQSRVMKEDVLRADREAQYASMAQPEDRRIPL
                     TTMRKIIGERMSESAHTAPTVNFTISVDMTNLKALREELKSERSLTYTDLIVKVTSLL
                     LLEHPYLNCSLDGEEIILRNYVNMGVAVALEDGLLVPVVKNAHMKKLGVISDEIKDLS
                     YRAKTNQLLTDEIEGGTFTITNIGMFGIESFTPIINQPESAILGVNAIIDTPVVVGGC
                     VCTKPIMKLSLTADHRTVDGAVAAMFLARLKEMLEHPLKILL"
     gene            2357..3742
                     /gene="lpdA"
                     /locus_tag="VFD79_08435"
     CDS             2357..3742
                     /gene="lpdA"
                     /locus_tag="VFD79_08435"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HZH94120.1"
                     /translation="MKIAIIGGGPGGYVAAVKAAQHGAQVVLVEKEHIGGTCLNTGCI
                     PTKILLHSTDIFHQLKNEAGALGLEMDNLRVNWQELMVRKDLVATQLVEGVKNLLKSG
                     NIEVVEGNARFASRTSLHVELAGGGSKRIDFDRAIVATGSESIVIPLPGHDLEGVITS
                     REAMSLEEIPERLCVIGGGVIGVEFANIYSNLGTEVTIVEMLPQLVANMDNEIVQGLE
                     DALIDGGVKIHKSAKVLKIEKADGGLEVWAQMGGDSFQVKADKVLMATGRRPAGDGLG
                     LEEIGVRVEKGAIQVDRLFKTDVDNIYAIGDCTGGVQLAHVASASGLMVADLIMGERK
                     AMDFRTIPYAVYTKPELAAVGMTENEALEKGYRIKVGRFPMFGNGKAVITGDVRGLVK
                     IVADERTGEVLGVHIGGSSATELIGVAALAIRLEATVDELLTTIYAHPTVGEAIHEAA
                     EGVFGKAIHLP"
     gene            complement(3988..4464)
                     /gene="ybaK"
                     /locus_tag="VFD79_08440"
     CDS             complement(3988..4464)
                     /gene="ybaK"
                     /locus_tag="VFD79_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005398778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HZH94121.1"
                     /translation="MSDKTNAARLCDNAGVEYVLIPYETDDDNVDGKSVAKKLGLWED
                     EIFKTLVTIGKSGEIYVFVIPVSGELDLKKAAKASGEKSIHMLPLKELLTVTGYVKGG
                     CSPLGMKKHYPTIFDETIILLDRITFNAGKVGLQLQLSVVDLNKVLTYNIAGLLKD"
     gene            4542..>6101
                     /gene="abc-f"
                     /locus_tag="VFD79_08445"
     CDS             4542..>6101
                     /gene="abc-f"
                     /locus_tag="VFD79_08445"
                     /inference="COORDINATES: protein motif:HMM:NF000355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HZH94122.1"
                     /translation="MIQCSVWKLKKYYGAEMVFENISFDLQAGERVGLIGQNGCGKTT
                     LMKILMGEENYQEGTLTFGKGVTLGYLHQIFKSPSGTTVREVLEQAFENIWDIKKQLR
                     DAEMAMKELSGRQLEKSMARYSSLVEKYELYGGYDVEVNMDKVCQGLRIPQDFMKKPF
                     DQLSGGEGTRVLLARLLLQEPDVLLLDEPTNHLDMDSIEWLEEFLIQYRGTVLMVSHD
                     RVFLDRVVDRIIELEPARVSSYNGNYTYYVEEKERRFQIEYREYMNNQRKIDMMERQI
                     ERYRIWGAMRDSEKMYKRAKELEKRLDKLEVVDKPQEEKRKIRLDGSGVQRTGKIVLD
                     VEALSKSFPGKSLFRNLTFTLYYQDRLCIMGANGSGKSTLLNIILGEAIQDNGSIKCG
                     SRVKIGYLPQNISFQNEDSTILEYFTKTHGITDQESRNELAKLLFFGDDVFRRIRVLS
                     GGEKSRLKLCSLLYTKINLLILDEPTNHLDIDTREVLENTLQDFEGTILFVSHDRYFV
                     EKLSTRIIVLEK"
BASE COUNT         1874 a         1115 c         1641 g         1471 t
ORIGIN      
        1 tgcctccccc caaggggtga gaagatgagt acaattggta ttattgcaaa tcctgcatcg
       61 ggcaaggaca tcagacggct tgtctcccat gcaactgttg tggataacaa tgaaaaggtt
      121 aatattgtaa agaggataat attggctgct cagggtgccg gagttacagg tgttatggtt
      181 atgcctgaca catttcttat aggatataag gcccaggagg atcttaaggg gtcaagggag
      241 ctgaatatac ctattgaaat actggatatg agagtcaagg ggagtgttga ggatactata
      301 cttgcggcaa aactgatgga ggaaaatggt gtccagtgca taattgccct tggaggggat
      361 gggaccagcc gggcagttgc aaaatcaatc acggtaacac cccttattag catttccaca
      421 gggacaaaca atgtctaccc tgaaatgctg gagggaactg tggcaggtat tgctgcggcg
      481 gctgtttcaa ccagaaccag caacctagga cagtcctgta gaagggacaa gagaatagaa
      541 atatacaagg gtggtaagat gctggacatt gctcttgttg actgtgtgat ttccaagcag
      601 acctatattg gttccagggc aatatggaat ccggaagata ttcaaagggt aatagtgtca
      661 agagcgaacc ctgcttcaat tggattttcc accatagtag gtgtcaagaa aatagttggg
      721 gaagatgata actttggagc atccatcaat gttaacacag gagagaatga gctggttgca
      781 cccattgccg ctggaacaat gaaggaaatc agagtggatg agccggtaat ccatcccctg
      841 gaggaaatgt tctcaatgac catggactac aagggaataa tggctcttga tggtgaaagg
      901 gagctgccct ttaaaaaggg agaaacatac cattttaaga taagcaggga tgggccctac
      961 agggtcaaca taaaaaggac cattgaatct gcacagataa acggattctt taaccggtga
     1021 gaccggagga ggaggtaaaa tggcaaagaa gatagtgatg cccaagctgg gcctgacaat
     1081 gaaagaggga aaactcgcca aatggtataa gcaggaggga gatcaggttc aggtcgggga
     1141 taaattattc agcatagaaa cagacaagat tacaaatgat gcagaagcta cagaagcagg
     1201 aatacttaga aggataatag tccaggatgg gcaaacagtt ccgataatgg agcctgttgg
     1261 tattgttgca ggagcggatg aggacatatc ctccctactg tctgaggtac ccgagacttc
     1321 aagtgacaaa acagtggaga aggttcaggt ggggaaaaag gctccagagc ctgagaagaa
     1381 aattgaaaga acaggtaagg tcaagatttc gccagtggca aagaagctgg cggaggagga
     1441 aggaatagat tttgagtcaa tccaaggaac cggtccaggc ggaaggattg ttcttgacga
     1501 tgtaaaaaat gcaattgaaa acaagggtgg gacaaaggca agtcccgcgg ctgagaagat
     1561 ggcaaaggaa aaaggaattg acatttcagg aatttccagc caatccaggg tgatgaagga
     1621 ggatgttctc cgtgcagaca gggaagctca atatgcatcc atggcccagc ctgaagacag
     1681 aaggattcct ttgaccacca tgagaaagat cattggtgaa agaatgagcg aaagtgctca
     1741 taccgcacca acggtcaact ttacaatatc agttgatatg acaaatctca aggcactgcg
     1801 ggaggagctg aaaagtgaga gaagtttaac ctacacagac ctgattgtga aggtgacatc
     1861 ccttcttctt ctggagcatc cttatctgaa ctgctcactg gacggagagg aaatcatact
     1921 gaggaactac gtcaatatgg gagttgcagt ggcactggag gatggacttt tggtaccggt
     1981 agttaagaat gcccacatga aaaagctagg ggtaatatcc gatgaaataa aggacctgtc
     2041 ctacagggca aagacaaatc agcttctcac agatgagata gaaggtggta ccttcaccat
     2101 tacaaatatt ggaatgtttg ggatagaaag cttcacacca ataattaatc aaccggaatc
     2161 agcaatactt ggagtcaatg caatcataga taccccagtt gtagttggag gctgcgtatg
     2221 cactaaaccc atcatgaagc tgtctcttac tgctgaccac agaacagttg acggtgcagt
     2281 tgcagccatg ttcctggcaa ggttgaagga aatgctggag catccactga aaatactgct
     2341 ttaggaggga gatactatga agattgcaat aatcggaggt gggccgggag gttatgtggc
     2401 cgcagttaag gctgctcaac atggagccca ggtcgtcctt gtggaaaagg aacacattgg
     2461 tgggacgtgc ctgaatacag gctgtatccc aaccaagata cttctccatt caacggatat
     2521 tttccaccag ttgaaaaatg aagccggggc acttggtctg gaaatggaca acttaagggt
     2581 taattggcag gaactcatgg ttagaaaaga ccttgtggcg acacagcttg ttgaaggagt
     2641 caagaacctt ctcaagtctg gcaatattga ggttgtggaa ggaaatgcca ggtttgcttc
     2701 aaggaccagt ctccatgtgg aacttgcagg tggtggaagt aaaaggatag actttgatag
     2761 ggccattgtg gccactggat ctgaatcaat agtcataccc cttccaggcc atgacctgga
     2821 gggagtcata acaagccggg aagccatgag cctggaggaa attccagaga gactgtgtgt
     2881 aatcggtggt ggtgtaattg gggttgaatt tgccaacata tactcaaatc ttggaacaga
     2941 ggttactatt gttgagatgc tcccccagct tgttgcaaat atggataatg agatagtcca
     3001 aggtcttgag gatgcactga ttgatggagg ggttaagatc cacaaatcag caaaggtcct
     3061 taagatagaa aaggccgacg gaggcttgga ggtatgggcc caaatgggtg gtgattcctt
     3121 ccaggtgaag gcagacaagg tattgatggc aactggtaga aggcctgccg gggatggatt
     3181 gggacttgag gagattggag tcagggtaga aaaaggagcc atccaggttg acagactatt
     3241 caagaccgat gttgacaata tctatgcaat aggtgactgc actggaggag tccagctggc
     3301 acatgtggct tctgcatcgg gattgatggt tgcagacctg ataatgggag agagaaaggc
     3361 catggacttc aggaccatac catatgcagt ctataccaag cctgagcttg cagcagtggg
     3421 aatgaccgag aatgaagcct tggaaaaggg ctataggata aaggttgggc gctttccaat
     3481 gttcggcaac ggtaaggcgg taattacagg tgatgtgaga ggtcttgtaa agatagttgc
     3541 agatgagagg actggagagg ttttgggagt tcacataggt ggaagtagtg caacagagct
     3601 tataggtgtc gccgctcttg caataaggct tgaggcaact gtggatgagc tcctaacaac
     3661 tatttacgct cacccaacag ttggtgaggc aatacacgaa gcagcggaag gggtttttgg
     3721 aaaggcaatc cacctccctt aaaacaaaaa ctgaatttaa gtacaaaaaa ccagagcatg
     3781 tattataact ctggtttttt cttgtgaatt gtgatttgtt cattgtgaac tggaagttcg
     3841 tgttataata aatgcaaaag caaattttgg aggtaatcat atgaataatt tcaataaggg
     3901 gcctgacccc agattcaggc tagttataat ttttgctgca cttggtgtgg ttttccttct
     3961 tgcatcaatt attgttggat acctgggcta gtccttgagc agaccggcaa tattgtaggt
     4021 aagaaccttg tttaggtcaa ccacggaaag ctgaagctga agacccacct tgcctgcatt
     4081 gaaggttatg cggtccagaa ggatgattgt ttcatcaaaa atagtaggat agtgcttttt
     4141 cattcccaga ggagagcaac cgccctttac ataacctgtt acagtaagga gctcctttag
     4201 gggtagcatg tgtatggatt tttcaccact ggccttggca gcctttttaa ggtccagctc
     4261 acctgagacc ggaatgacaa aaacatagat ctctccgctt ttgcctattg taaccagggt
     4321 tttgaatatt tcatcctccc ataggccaag tttttttgcg acggatttgc catcaacatt
     4381 gtcgtcatca gtctcatagg ggatcaatac atattcaaca ccggcattgt cacatagcct
     4441 tgcggcgttt gttttatcag acattgcaat caactccttt agtatatgat aatacaaaac
     4501 atcaaaatca gcaaagacaa gatagattgg aagtgatgca catgatacag tgcagtgtat
     4561 ggaagcttaa gaaatactac ggagccgaaa tggtttttga aaacatatca tttgacctgc
     4621 aggcaggaga aagagttggc ttgattggtc agaacgggtg tggaaaaacc actttgatga
     4681 aaattctaat gggtgaggag aattatcagg agggaaccct tacatttggt aaaggagtta
     4741 ccctgggata ccttcatcag attttcaaga gcccgtctgg aacaacagtt agggaggttc
     4801 tggaacaggc atttgagaat atatgggaca taaagaaaca gctgcgtgat gcggaaatgg
     4861 ctatgaagga actttcaggt aggcagcttg agaaatccat ggccaggtat tcatccctgg
     4921 ttgagaaata tgagctctac ggtggatacg atgttgaagt aaatatggat aaggtttgcc
     4981 agggattgag aatacctcag gattttatga aaaagccctt tgatcagttg agcggtggag
     5041 aaggtaccag ggtgctcctt gcaagactcc tcctacagga gccggatgtc ctgctccttg
     5101 acgagccgac aaaccatctt gatatggatt caatcgagtg gctggaggaa tttctgattc
     5161 aatatagggg aactgtattg atggtttccc acgacagggt ttttctggac agggttgttg
     5221 acaggataat tgaacttgag cccgcaaggg tcagtagcta caacgggaac tacacatact
     5281 acgtggagga gaaggaaaga cggttccaaa ttgaatacag ggagtatatg aacaaccagc
     5341 gaaagattga catgatggag aggcagattg agaggtaccg aatatggggt gccatgagag
     5401 acagtgaaaa aatgtacaaa agggccaagg agcttgaaaa gagattggac aagcttgagg
     5461 ttgttgacaa gcctcaggag gaaaagagaa aaatccgtct tgatgggtca ggagtacaaa
     5521 gaactgggaa aatagtgctg gatgtggagg ctctttcaaa atccttcccg ggtaaaagtc
     5581 tattcaggaa tctgacattc accctatatt atcaggacag gctatgtata atgggtgcaa
     5641 acgggtctgg aaagagtacc ttgcttaata ttatccttgg ggaggctatc caggacaatg
     5701 gaagcataaa gtgtgggtca agggttaaaa tcgggtatct tccacagaat ataagttttc
     5761 agaacgaaga ttcaacgatt cttgagtatt tcacaaaaac ccatggcata accgaccagg
     5821 agtccagaaa cgaactggct aagcttctct tctttggaga tgatgtcttc agaaggatca
     5881 gggtcctgtc aggaggagag aagtcaaggt tgaagctttg ctcacttctt tacacaaaaa
     5941 taaatctcct gattcttgac gagcccacca accacttgga tattgacacc agggaggtac
     6001 ttgaaaacac cctccaggac tttgaaggga caattctctt tgtatcccac gacaggtact
     6061 ttgtggaaaa gctttctaca aggattattg tacttgagaa g
//
LOCUS       DASRQF010000319         4622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_6958, whole genome shotgun sequence.
ACCESSION   DASRQF010000319 DASRQF010000000
VERSION     DASRQF010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6958"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VFD79_08450"
     CDS             <1..494
                     /locus_tag="VFD79_08450"
                     /inference="COORDINATES: protein motif:HMM:NF015656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine / lysine / ornithine decarboxylase"
                     /protein_id="HZH94123.1"
                     /translation="IFDKDNTKLLINIDGIRGTVLKRMLLKDYNIRIEMSDFYYALVL
                     TSVMNTEEEYETIVSALEDIAEKAPYEEINWVDVKMPTPKVIMRPSEAYYSKKEQIKL
                     EDAIGRVSAAPIIPYPPGIPLLVPGEEITQEIYEHLLFLMDNGMDIVGLMGYNKDYVV
                     VTE"
     gene            508..1143
                     /gene="tmk"
                     /locus_tag="VFD79_08455"
     CDS             508..1143
                     /gene="tmk"
                     /locus_tag="VFD79_08455"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HZH94124.1"
                     /translation="MRGKFIALEGPDGSGKSTMMNLIDQYLVEHQIDHVTTREPGGTA
                     IGEKIRHLIVDRDNANMGPETEALLFAASRAQHVHEKILPALDQGKAVVCDRFLLSSL
                     AYQGVGRGLGVDQVKAINDFGLRGVYPDLILFFHVDPEVTLLRKTKEGGDRLEAEGGL
                     FHREVYEGYMTLLEKYPENITLVDAAKPIDEVYRQTIQAIDKLLRPKEEKR"
     gene            1140..1466
                     /locus_tag="VFD79_08460"
     CDS             1140..1466
                     /locus_tag="VFD79_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HZH94125.1"
                     /translation="MKLIVAIVQDQDAQNVINELTEKEFRVTKLASTGGFLKAGNTTL
                     LSGVENDQVDEVVEVIERNCKTREITTSLLTVTMPGDTYIPYPLEVKVGGATVFVLDV
                     EQHIKI"
     gene            1477..1758
                     /locus_tag="VFD79_08465"
     CDS             1477..1758
                     /locus_tag="VFD79_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HZH94126.1"
                     /translation="MKLIIAIIDEQYSNKVVRKLMSEKIRTTKLASTGGFLSKGNTTL
                     LIGVDDDQVDRISELIAGICKKQKVQNGDEQVTVGANLFVVPIDSYQRV"
     gene            1773..2753
                     /gene="holB"
                     /locus_tag="VFD79_08470"
     CDS             1773..2753
                     /gene="holB"
                     /locus_tag="VFD79_08470"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HZH94127.1"
                     /translation="MDYKDIVGHEWVVQSLKRAVKNNNISHFYLFEGEEGLGKRTLGR
                     TFAKTLLCGNKGEEPCNTCSSCMKFDHGNHPDYHEIEPDKGIIKKGQVEEVIRGMSMS
                     PFQGDRKVFLMDQAHLMNKEAQNAMLKSLEEPPAYVHMILVTSSPKNLLPTIMSRGQQ
                     IRFGQIPVSLVADHLVQREGVEMSQAKFLADFSKGSLGRAVQLSDSEDFFQLRDWIID
                     LIDSSIKGESWKIFSAVERFTDEKDRVQELLDIMLFWFRDLMVYKSTGDMGLVINSDK
                     ELLLSEQSFLDFERINGIIKKIQDTSVNIQLNVNYQLSIETMLFKIQEEF"
     gene            2753..3643
                     /locus_tag="VFD79_08475"
     CDS             2753..3643
                     /locus_tag="VFD79_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009221413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HZH94128.1"
                     /translation="MIKVVGVRFKKAGKIYYFDPDKIEVQNNDFVIVETARGVEFGHV
                     VVGPKEVTEEEIVTPLKKVLRIALDEDFDTHRENKKKAKEALEICEKKVADHNLDMKL
                     VDVEYTFDNNKVIFYFTADGRVDFRDLVKDLASIFRTRIELRQIGVRDEAKMIGGVGP
                     CGQVTCCTRFLGEFAPVSIKMAKEQSLSLNPTKISGLCGRLMCCLKYEQDTYEELLEK
                     MPNVGDIVTTPQGRGIIVDTYTLLERVKAKVKLQDGTEDILQFQVDEIQRTGKYDSSY
                     QRYHNNNRNSKDEDVRGLED"
     gene            3740..3907
                     /locus_tag="VFD79_08480"
     CDS             3740..3907
                     /locus_tag="VFD79_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZH94129.1"
                     /translation="MAYIINDDCIACGACEPECPTEAISPGDIYVIDADKCIDCGACA
                     AVCPVDAPKPE"
BASE COUNT         1497 a          852 c         1194 g         1079 t
ORIGIN      
        1 caatctttga caaggacaat accaagcttt tgataaacat agatggaatc agggggactg
       61 ttctcaagag aatgctcctt aaggactaca acattaggat cgagatgtca gatttctact
      121 atgcacttgt ccttaccagc gtcatgaaca ctgaggagga gtatgaaacc atagtcagtg
      181 ctttggagga catcgcggaa aaggcgccct atgaggagat taattgggtg gatgttaaaa
      241 tgccaacccc caaggtaatc atgagaccat ccgaggccta ctacagcaag aaggagcaga
      301 ttaagctgga ggatgccatc ggaagggtat cagcggcgcc aataatacct tatccacctg
      361 gaattccact gcttgtacca ggcgaggaaa tcacacagga aatctacgaa catcttctct
      421 tcctaatgga caacggtatg gatatcgttg gtctaatggg gtataataag gattatgtag
      481 tagttactga ataaggagtt ggtggatatg aggggcaagt ttatagcgct ggagggaccg
      541 gacgggtcgg gaaagtcaac catgatgaac ctgatagacc aatacctggt tgagcatcaa
      601 attgaccacg taacaacccg ggaacccggt ggaacagcaa ttggagagaa aatcagacat
      661 cttatagtag acagggacaa tgcaaacatg ggacctgaaa cggaggccct cctatttgct
      721 gcatccagag cccagcatgt tcatgagaag atactacctg cccttgacca gggaaaagcc
      781 gtggtttgtg acaggttcct gctttcaagc cttgcctacc agggagttgg aagagggctt
      841 ggggtggacc aggtcaaggc cataaacgat tttggtttaa ggggggtata ccctgatttg
      901 atactcttct tccacgttga tcccgaggtg acacttctga ggaagacaaa ggaaggcgga
      961 gacaggcttg aggctgaggg gggcctcttc cacagggagg tctacgaagg ctatatgacc
     1021 ctgttggaga aatatcccga gaatatcacc ctagtggatg ccgccaagcc cattgatgag
     1081 gtttacaggc agacaatcca agccatagac aagcttttga gacccaagga ggagaaaaga
     1141 tgaaactgat tgtagctata gttcaggacc aggatgcaca aaacgtgata aatgagctaa
     1201 ctgaaaagga attcagggtt accaaacttg cctcaaccgg tggatttcta aaggctggaa
     1261 acacaacact cctaagtggt gttgagaatg atcaggtgga tgaggtagtt gaagtcattg
     1321 aaagaaactg caagaccagg gaaatcacca catcactgct gaccgtaaca atgccagggg
     1381 atacatatat tccatatcct ctggaggtaa aggtaggagg ggcaacggtg tttgttctgg
     1441 atgtggagca gcatataaag atttaggagg tcccatatga agctaattat cgccatcatt
     1501 gatgaacagt actcaaacaa ggtagtaaga aagcttatgt ctgaaaagat aagaaccaca
     1561 aaactggctt caaccggtgg tttcctgtca aagggaaaca caaccctgct gattggtgtg
     1621 gatgatgacc aggtggacag gataagtgag cttatagctg gaatctgcaa gaagcagaag
     1681 gttcagaatg gagacgagca ggtaacagtg ggagccaacc tttttgttgt tcccatcgac
     1741 agctatcaga gggtgtagtc tggaggagtt ctatggatta caaggatatt gtgggccatg
     1801 aatgggtggt ccaaagtctt aagagagcgg ttaaaaacaa taatattagt cacttctatc
     1861 tgtttgaagg agaggaaggt cttggtaaaa gaacattagg gaggactttt gccaagactc
     1921 ttttgtgtgg gaacaaggga gaggagcctt gcaacacctg cagctcctgc atgaaatttg
     1981 accacggaaa ccatcctgac taccatgaga tcgagcctga taagggaatc ataaaaaaag
     2041 gtcaggtcga ggaggtaatc aggggcatgt ccatgtcccc attccagggg gacaggaagg
     2101 tgtttctcat ggaccaggca caccttatga acaaggaagc ccagaatgca atgctcaagt
     2161 cccttgagga gccacccgcc tacgtacata tgattttagt gacatcaagc cccaagaacc
     2221 tccttccaac cataatgtcc aggggacagc agataaggtt tggccagatt cctgtgagcc
     2281 tagttgcaga ccatttggtc caaagggaag gtgtggaaat gagccaggca aaattccttg
     2341 cagacttttc caaggggtcc ctgggaaggg cggtccagct gtccgattct gaagatttct
     2401 tccagctgag ggattggatt attgacctta ttgattcctc aattaaaggt gagtcctgga
     2461 agatattttc agctgttgaa aggttcactg atgaaaagga cagggtccag gagcttttgg
     2521 acattatgct cttctggttc agggacctta tggtctacaa gtcaacagga gacatgggcc
     2581 ttgttataaa cagcgataaa gagttattgt tatcagaaca atctttcctg gatttcgaga
     2641 gaataaatgg tataataaag aagatacagg atacgtccgt gaatatacag ctaaatgtta
     2701 attaccagct gtccatagag acgatgttgt ttaaaataca ggaggagttt tgatgataaa
     2761 agtagtagga gtgagattta agaaagccgg gaagatttat tatttcgatc ccgataagat
     2821 agaggttcag aacaatgatt ttgtaatcgt tgagactgca aggggagttg agtttggcca
     2881 tgtggttgtt ggacccaagg aggtcactga ggaggaaata gtgacacccc ttaagaaggt
     2941 acttagaatt gcccttgatg aggactttga cacccacagg gaaaacaaga agaaggccaa
     3001 ggaagccctt gaaatatgcg aaaagaaggt tgccgaccac aatctggaca tgaagctagt
     3061 ggatgtggag tatacctttg acaacaacaa ggtgattttc tactttacag ccgatggaag
     3121 ggtagacttc agggacctgg tgaaggacct ggcgtcaata ttcagaacca ggatagagct
     3181 taggcagatc ggagtaaggg acgaagcaaa gatgatcggt ggagtgggac cctgcggaca
     3241 ggtaacctgc tgtacaagat tcctgggtga atttgcccct gtatcaatca agatggcaaa
     3301 ggaacaaagc ctgtccttga acccaaccaa gatatccggt ctttgcggaa ggctcatgtg
     3361 ctgcctcaag tacgagcagg acacctatga ggaacttctt gaaaagatgc cgaatgttgg
     3421 ggacatagtc acaacacccc agggaagggg aatcattgtg gacacctaca ccctccttga
     3481 aagggttaag gcaaaggtta agctacagga tggaacagag gatatcctgc agttccaggt
     3541 tgatgaaatt caaagaaccg gcaaatatga ttccagctac cagagatacc acaacaacaa
     3601 tagaaattcc aaggacgagg atgtaagagg cctggaggat tagcaagttt ttaatatatt
     3661 ttgatgttac atcttttaaa agctagttat ttctgttaaa atataaccaa gcaatttcat
     3721 tttacaggag gtgaaaagaa tggcttatat aattaatgat gattgtatcg catgtggagc
     3781 atgtgaacca gaatgcccaa cagaagcaat atctcctggc gatatctatg ttatcgacgc
     3841 agataaatgc attgactgtg gcgcttgtgc tgctgtatgt ccagtagacg caccaaagcc
     3901 agagtagtac ttagaatcct aagaagaccc gtatatgggt cttttttagt acagatatag
     3961 aaatacagtg tacaacgtac aatgaatagt gaaaaatctg ttattgtcat tccgagcgta
     4021 gtcgagacat ctcgggtatt tagttaatat ctaagaggta agaggtaaag acagtgaaca
     4081 gtgaacagtg aacagtgaac aatgagcaaa tctaagaatt aagatctaag agttaagagc
     4141 ttgaaaacca caatcattgt cactctgaac gaagtgaaga gtcttaatgc tttgaactat
     4201 agacaaagaa agatccttcg ctgcactcag gatgacaaaa tgtcatgtcg agcgtagtcg
     4261 agacatctcg ggtatttagt taatatctaa gaggtaagag ttaaagacag tgaacaatga
     4321 acagtgaaca atgaacagtg aacaaagaac agtgaacaaa gaacagtgaa caaagaacag
     4381 tgaacaaaga acagtgaaca aagaacaaag aacagtgaac aaagaacaaa gaacagtgaa
     4441 caaagaatta ggtaagaagt aagagttaaa aatcaatgga caattaacaa tgaacaatga
     4501 acagtgaaca gtgaagaagt aaaagaagtg gtcattccga gcctagtcga ggaatctaag
     4561 gtcttgagac ccctccgctt cggtcggggt gacgtatttt ttgtggagtg tgttagagat
     4621 at
//
LOCUS       DASRQF010000320         3305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1429355, whole genome shotgun sequence.
ACCESSION   DASRQF010000320 DASRQF010000000
VERSION     DASRQF010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429355"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1506)
                     /locus_tag="VFD79_08485"
     CDS             complement(<1..1506)
                     /locus_tag="VFD79_08485"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HZH94130.1"
                     /translation="MSTENRKARKQNRFTLGKAIKIFLVMIIIAGIIAGGTVGAAVVS
                     ILDEAPEIDPTTIVTSLAETSSIYDPDGNLIEKIQAEELRTVVGIKDMPQHLIDAFIA
                     IEDHRFEEHPGVDIEGIAGAVLDNFRSGGLRGASTITQQLVKNVYLSNEVTLTRKITE
                     AYLALKVETILSKDQILEAYLNRNYFGQNAYGVQEASRTYFSKNVQDLTLAESALLAG
                     IVKSTTQFQPYYRVKPGDFNPEVHYEVGQLDVLGEPYIAVFNEESVNRQRIVLDRMLA
                     QEKITESQYNSALAQDIKSSLKPEEKKPQDITSYFADLVKTQVVRALVDKLGYTREQA
                     ESELFTGGLSIYATIDMDMQKQLEDIYSNFVEILVGNVDGVRGPVLVDWRINSVGNVI
                     DERNRIVYYKKDLLMTEENNLILEDGEFEVENGSLKINTNKINIFPTHFDIADFYTID
                     DKKNLVAHSVGSIVIPEGQFTVEDGIITIDKSYLDNNPDFYQTQDGRAIVSN"
     gene            1675..2946
                     /gene="hflX"
                     /locus_tag="VFD79_08490"
     CDS             1675..2946
                     /gene="hflX"
                     /locus_tag="VFD79_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HZH94131.1"
                     /translation="MDDSKEKVLIVGVEIDSDRVDIDDSLNELEELVEAAGGKVEARI
                     IQRKDKINAATFIGTGKAQEIQSYCEDLEIDTVVFNDELSGAQLRNLEEIIKRKIIDR
                     TTLILDIFALRAVSKEGKLQVKLAQLKYRLPRLVGFRDYLSREGAGIGTRGPGEQKLE
                     TDRRHILREINNIERQLKDLEGVRDVKRKKRISSQMPMVALVGYTNAGKSTLMNLLMT
                     QKEGSEDNKQVYVEDMLFASLDTAHRVGKLPNGQDYLLADTVGFVSKLPTKLVEAFKG
                     TLEEVEYADLLLHVVDASNKDLDIQINTTMDLLKDLETVDKPIVTVFNKMDRLREKGI
                     NIVGMETDNPIYISAIEGQGVEELKQLIQDTLPLEYRRVRMMIPFDRQSVSSYLMNQY
                     QVDEIEYTEEGTLFTLTINHIDYEKHKEFII"
     gene            2970..>3305
                     /locus_tag="VFD79_08495"
     CDS             2970..>3305
                     /locus_tag="VFD79_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002839087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HZH94132.1"
                     /translation="MKETYRTIARRGADEFIVNKSRFIGYAAPVTTEDQALEFIQEIK
                     TKHKDATHNVSAYVLGLDSNIQRFNDDGEPSGTAGIPALEVLKKEDLRNTVVVITRYF
                     GGIKLGGGGL"
BASE COUNT          941 a          698 c          771 g          895 t
ORIGIN      
        1 gttgcttaca atggcccttc catcctgggt ctggtaaaaa tcagggttgt tgtcaagata
       61 tgacttgtcg atggtaataa tcccatcctc aacggtgaac tgaccctctg gaataacaat
      121 tgatcctaca ctgtgtgcta caagattctt cttgtcatcg atggtataga aatcagctat
      181 gtcaaaatgt gttgggaaga tgttgatctt gtttgtattg attttcaaac tgccattttc
      241 aacttcaaac tcgccatctt ccaaaatcag gttgttctcc tctgtcatca acagatcttt
      301 tttgtagtat actatcctat ttctttcatc aataacattt cccacgctgt ttattctcca
      361 gtccacaagg actggtcccc ttacaccatc aacatttcca actagaattt ccacaaagtt
      421 tgagtagata tcttcaagct gcttttgcat atccatgtca atggttgcat atatggacaa
      481 accaccggtg aatagctcgc tttctgcctg ttccctcgtg tatccaagct tatccacaag
      541 agcccttaca acttgagttt tcaccaggtc agcaaagtat gaggttatgt cttgaggttt
      601 cttttcctca ggcttcaggc tggatttaat gtcctgggcc aaggctgaat tatattgact
      661 ttcagtaatc ttttcctggg ccagcatccg gtccagtaca attctctgcc tgttaaccga
      721 ttcctcatta aatacagcaa tgtagggctc acccagtacg tcaagctgtc caacctcgta
      781 gtggacctcc ggattgaagt ccccaggctt aaccctgtag tatggctgga actgagttgt
      841 actcttcact ataccagcaa gcaaggctga ctcagccaga gtcagatcct gcacattctt
      901 tgagaagtat gttcttgagg cttcctgaac cccataggca ttctggccga aatagtttct
      961 gtttaggtaa gcctccagta tctggtcctt tgaaagtatg gtttcaacct ttagggccag
     1021 ataggcctcg gtgatttttc tggtaagggt aacctcgttg cttaagtaaa cgttctttac
     1081 aagctgctgt gtgattgtac ttgcacccct aagtcctcca gacctgaagt tgtccaaaac
     1141 cgcacctgca attccttcga tgtcaacgcc gggatgttct tcgaacctgt ggtcttcgat
     1201 tgcaataaag gcatcaatca ggtgctgtgg catgtccttt atgccaacca ccgtcctaag
     1261 ctcctctgct tggatctttt caatcagatt gccgtccggg tcgtatatgc tggatgtttc
     1321 agccagtgat gttacaatgg tagtagggtc aatctccggc gcctcatcca ggatggatac
     1381 aacggcagct cccacggtac caccggctat tatgccggca atgatgatca tgaccagaaa
     1441 gatctttatt gcctttccaa gggtgaatct attctgtttt cttgcttttc tattttcagt
     1501 agacattgtt aacctcctaa agttttcact acaaaagata acatattata ccacagaaca
     1561 ctttttttgt taagctatgg aaggttacaa taagattacg gaaggttgct tttaactggt
     1621 ctaagagcct taaaaatggt atacttaaaa catcctgaca ggagagtgat gataatggat
     1681 gatagcaagg aaaaggtatt gatcgtaggg gtcgagatag acagtgatag ggtggacatt
     1741 gatgattcac tgaatgaatt ggaggagctt gtggaggccg caggcggcaa ggtggaagcc
     1801 agaataatcc agaggaagga taaaatcaat gcagcaacct tcattgggac tggaaaggca
     1861 caggaaatcc aatcctactg tgaagacctt gaaatagata cagtggtttt caatgatgag
     1921 ctttcaggtg cccagctacg aaatctggag gagataatca agagaaaaat cattgatagg
     1981 actacgctga ttttggatat atttgccctt agggcagtct ccaaggaggg taagctccag
     2041 gttaagcttg ctcagcttaa atacaggctg cccaggcttg ttggctttag ggactatctt
     2101 tccagggaag gtgctggcat tggtacaaga ggacctgggg aacaaaagct tgagacggac
     2161 agaagacaca tactcaggga gataaacaat attgaaaggc agctgaagga ccttgaaggg
     2221 gtaagggatg ttaaaaggaa aaagaggata tcctcccaga tgcccatggt cgcacttgtt
     2281 ggctatacaa atgctgggaa atcaaccctg atgaacctgc ttatgactca gaaggagggc
     2341 tcagaggaca acaagcaggt atatgttgag gatatgctct tcgccagtct tgacacagcc
     2401 catagggttg gcaagctacc caatggccag gactatctgc ttgcagacac ggtaggcttt
     2461 gtatcaaagc ttccaaccaa attggtggag gcattcaagg ggactcttga ggaggttgaa
     2521 tatgcggacc ttctgcttca tgtagtggat gcatcaaaca aggatcttga tattcagatc
     2581 aacacaacaa tggacctttt gaaggacttg gaaacagtgg acaagccaat tgttacagtt
     2641 ttcaacaaga tggacaggct aagggaaaag gggatcaata tcgtgggaat ggaaactgat
     2701 aaccccattt atatctctgc catagagggc cagggagtag aagagcttaa gcagcttatc
     2761 caggacaccc tgcctcttga atacagaagg gtaaggatga tgataccctt tgacaggcaa
     2821 tccgtatcca gctacctaat gaaccaatac caggttgatg agatagagta tacagaggag
     2881 ggtaccttgt ttaccctgac aataaatcat attgactatg aaaaacataa ggagtttatt
     2941 atttaatgat gcgggaggaa gttgatggta tgaaggaaac ctacaggaca attgccagaa
     3001 gaggagctga tgaattcatt gtcaacaagt ctcgtttcat aggctacgca gctcctgtga
     3061 caacagaaga ccaggcactg gaattcatac aggaaatcaa gaccaagcat aaggacgcaa
     3121 cccacaatgt ttcagcctac gtactggggc ttgattcaaa catccagaga ttcaacgatg
     3181 acggtgaacc atctggaaca gcaggaattc cagcactgga ggtcctgaag aaagaagacc
     3241 tgagaaacac cgttgtggtc ataacaaggt atttcggagg gattaagctt ggaggtggag
     3301 ggctt
//
LOCUS       DASRQF010000321         4118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_439547, whole genome shotgun sequence.
ACCESSION   DASRQF010000321 DASRQF010000000
VERSION     DASRQF010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439547"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..171)
                     /locus_tag="VFD79_08500"
     tRNA            complement(98..171)
                     /locus_tag="VFD79_08500"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(137..139),aa:Cys,seq:gca)
     gene            complement(179..253)
                     /locus_tag="VFD79_08505"
     tRNA            complement(179..253)
                     /locus_tag="VFD79_08505"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(219..221),aa:Gly,seq:gcc)
     gene            complement(282..357)
                     /locus_tag="VFD79_08510"
     tRNA            complement(282..357)
                     /locus_tag="VFD79_08510"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(322..324),aa:Phe,seq:gaa)
     gene            complement(404..1291)
                     /gene="hslO"
                     /locus_tag="VFD79_08515"
     CDS             complement(404..1291)
                     /gene="hslO"
                     /locus_tag="VFD79_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HZH94133.1"
                     /translation="MKDYMIRGMDREGRLRVFVARTTQMVDEARKIHNTSATATAAMG
                     RLLTASAMMGATMKNDTDLLTLKVSGDGPVGTITAVANNRGEVKGLIDNPAADAPSTD
                     SGKLDVGAIVGKKGVFVSITDIGMRDPYIGQTSLVTGEIGDDIANFYSESEQIPTAVG
                     LGVLVDRDLSCKAAGGFIIQLLPFITDEEIGMIESSLEKARPLSTLIDEGYTPEEIME
                     ELLSEFNMETTDRIDVSYTCDCSADKVEKVLISLGEKEIGDIIEEDGESEVVCHFCNT
                     KYHFHKDDLEKILLTIKNK"
     gene            complement(1302..2048)
                     /locus_tag="VFD79_08520"
     CDS             complement(1302..2048)
                     /locus_tag="VFD79_08520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZH94134.1"
                     /translation="MDSYGEFALLYDDLMDDFDYVSWSRYIQDILTRKGFGEVSILEM
                     ACGTGNLTKELLNLGYKVDAFDLSHEMLAIANNKLANYKNKRLFKMDMKEFKLDRKYE
                     AVISACDSINYILDEESLKNTFTNVFNHLEDGGIFIFDINSEYKLREILGNNIFIEDR
                     EDVFYTWENQFQDETGICEFFLTFFQWTKGNDYRRFDEHHRERAYKVETVKRILGEVG
                     FHEIEAYEAFSFKDVEDKSERINFVAKKFK"
     gene            complement(2065..3021)
                     /locus_tag="VFD79_08525"
     CDS             complement(2065..3021)
                     /locus_tag="VFD79_08525"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HZH94135.1"
                     /translation="MRTRSLPTINEVNILFLVLGLVLLIFGSMAQQRDLYIGVLITEY
                     IFILAPNLIYLKLRGYSLRNVLGLNPITIRQSIQIILIMVFAYPMAVFLNYIVLVMVN
                     SVSPAMPIGVPAPANLIEYLIGLLVIAVTPGICEEVMFRGTMQAAYTRMGERKAILIS
                     SMLFGMFHFNLLNLLGPLFLGIVLGIIRFKTNSLYGSILGHTINNGIALTLGFLLAGF
                     TENIQEITEQAPLIPEGLQLMITLVMLGGWALFSFVVLYLLLKNLPGPGYKTRIETSM
                     DAIDSVEGHEDAAMDHYSISWAPIVVIACIFILLNYRYFFLI"
     gene            complement(3092..3613)
                     /gene="hpt"
                     /locus_tag="VFD79_08530"
     CDS             complement(3092..3613)
                     /gene="hpt"
                     /locus_tag="VFD79_08530"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HZH94136.1"
                     /translation="MDNKKEKILYSRDEIDVRVKEMGKELSQQYKGRDLVIVSLLRGS
                     FVFTADLSRELSIPVEIEFMTTASYGNEEVSSGNVKILQDVRGSLKGKDVLIVDDIID
                     TGHTLKKVIEHLEEKEPNSIKVCVMLDKPSRRQVDLEPDYVGYEIPDVFIVGYGLNYG
                     DYFRNIPYIFTFE"
BASE COUNT         1194 a          979 c          657 g         1288 t
ORIGIN      
        1 atcttgcttg gaacaaatgg gttaatcatg atatctcatc cttaaagtta tgttgatttc
       61 aaaaaaatcc ttctgaatta acagaaggat ttcattatgg aggcgacacc cagatttgaa
      121 ctgggggtga aggtgttgca gacctctgcc ttaccacttg gctatgccgc cgtattcttg
      181 gagcggaaga cgagattcga actcgcgacc ctcgccttgg caaggcgatg ctctaccact
      241 gagccactcc cgcataccat tattatacca taaaaccatt gtggtgccca gagccggaat
      301 cgaaccagcg acacgtggat tttcagtcca ctgctctacc gactgagcta tctgggcaag
      361 taatcgcacc atttctggcc gatgtcacaa tagctagtat acattattta ttctttattg
      421 tcaacaggat tttctcaagg tcatccttgt gaaaatggta ttttgtatta cagaaatgac
      481 agactacttc gctttcccca tcctcttcta tgatgtcacc tatctccttt tcaccaagac
      541 tgatcaggac cttctcaacc ttgtctgccg aacagtcaca ggtgtagctt acatcaatcc
      601 tatcagtggt ctccatgttg aattcagaca acagctcctc cattatctcc tctggtgtat
      661 atccttcatc aatcagtgtg gaaagcggtc tcgctttctc caaagaggat tctatcatac
      721 ctatctcctc atcggtaata aagggaagca actgaatgat aaaccctcct gctgccttgc
      781 aggaaaggtc cctgtcgact aagactccca ggccgactgc cgtggggatc tgctctgatt
      841 cgctgtaaaa attggcaatg tcatccccaa tttctccagt cacaagactt gtttgcccaa
      901 tatatgggtc cctcattcca atatcagtta ttgaaacaaa aacacctttc tttccaacta
      961 tcgcacccac atcaagcttt cctgaatctg ttgatggtgc atctgctgca ggattatcaa
     1021 tcaatccctt gacctctcct cggttgttgg caacagctgt gatggttcca acaggaccat
     1081 ctcccgatac cttaagtgtc aataggtcag tgtcattttt catggttgcg cccatcatgg
     1141 cactggctgt caacaatctc cccattgcag cggttgcagt agctgatgta ttgtgaattt
     1201 ttctagcttc gtccaccatt tgtgttgttc ttgctacaaa taccctaagt cttccctctc
     1261 tgtccatacc tcttatcata taatccttca taatatcacc cttacttaaa tttctttgct
     1321 acaaagttaa ttcgttcact cttatcctct acatctttga agctaaaagc ttcataggct
     1381 tcaatttcat ggaatcccac ttctcccagt attctcttaa cagtttccac tttataggcc
     1441 ctctcccggt gatgctcgtc aaaccttctg tagtcatttc ccttggtcca ctgaaaaaag
     1501 gtaaggaaga actcacaaat cccagtctca tcctggaatt gattctccca tgtgtagaat
     1561 acatcctccc tatcttcaat aaaaatgttg ttccccagga tttctctcaa cttatactcg
     1621 gaattgatat caaagataaa aatcccgcca tcttccaaat gattaaatac attagtgaag
     1681 gtgtttttca gagattcctc gtcaagtata tagttgatac tgtcacatgc agagatgaca
     1741 gcctcgtact ttcggtccag cttaaattcc ttcatgtcca tcttaaacag tctcttattc
     1801 ttgtaattgg caagcttatt gttggcaatg gcaagcattt catgtgaaag gtcaaaagca
     1861 tcaaccttgt agcccagatt taacaattcc tttgttaaat tgccggtacc acatgccatt
     1921 tcaagaattg atacttcacc aaatcccttg cgagtcaata tatcctgaat gtacctgctc
     1981 caggacacat aatcaaaatc atccatcaag tcatcatata gcaaggcaaa ctcaccgtaa
     2041 ctgtccatat tattcacacc cttcttatat gaggaaaaag tatctgtaat tcaaaagtat
     2101 aaaaatacac gcaataacca ctattggagc ccaagaaata ctgtagtgat ccattgcagc
     2161 atcttcatgt ccttcgactg aatctatagc atccatactg gtctcaatcc tagttttata
     2221 ccctggtcct ggtaggtttt tcagaagcag gtagagtaca acaaatgaaa acaaggccca
     2281 accacctaac ataaccaagg tgatcatcaa ttgaagtccc tccggtatca agggagcctg
     2341 ttcagtaatt tcctgtatgt tctcagtgaa tcccgccagt aagaaaccca aggtcaatgc
     2401 aatcccattg tttattgtat ggccaagtat ggacccatag aggctattgg tcttaaatct
     2461 aattatccct aggacaatcc ctagaaaaag tggtcccaac aggtttaaga ggttaaaatg
     2521 aaacattcca aacaacattg acgaaattag aatagccttt ctttctccca ttctggtata
     2581 tgcagcttgc atggttcccc tgaacataac ctcctcgcat atccctggag taacagcgat
     2641 cacaagaagt ccaatcagat actcgattag atttgcaggt gccggcacac ctatgggcat
     2701 tgctggactc actgaattga ccattacaag tacaatgtaa ttcaggaaaa ctgccattgg
     2761 atatgcgaaa accatgatta aaattatttg tatggattgt ctaattgtaa ttggattcaa
     2821 gccaagaaca tttcttaatg aatagcccct tagctttaga taaattagat ttggggctag
     2881 tatgaaaatg tattcagtta tgagaactcc aatataaagg tctctttgct gtgccattga
     2941 tccaaatatc aatagtacaa gacccagtac caggaatagt atgttgactt catttattgt
     3001 tggtaagctc cttgtcctca taatccacca cccggttttt catctcacat ccattaaata
     3061 cacctacagt tccctgtagg tgaaataaac tttactcaaa tgtaaatata taagggatat
     3121 ttctgaaata atccccataa tttagtccat aacccacaat aaatacatca ggaatttcat
     3181 atcctacata gtcaggttcc aaatccacct gtcttctgct gggcttatcc aacattacgc
     3241 acaccttaat tgagttaggc tccttttctt caagatgctc aatgaccttc ttaagagtgt
     3301 gtccagtgtc aattatatcg tccactatca gtacatcctt gcctttcaag ctacctctca
     3361 catcctgaag gatctttaca ttaccagatg agacctcctc gtttccatag ctagctgttg
     3421 tcataaattc aatctcaaca ggaatgctca gttctctgga aaggtcagcc gtaaatacaa
     3481 agcttcccct taatagggat acaattacaa ggtctcttcc cttgtattgt tgactcaatt
     3541 cttttcccat ttccttgact ctaacatcaa tttcatctct tgaatataga attttttcct
     3601 tcttattatc tatcaaacta gtttctcctt cctatttgaa tttatttctc aaaatatcct
     3661 tcaattccct taattccttt aaaatcaaat tcaaagtcaa ttgaatactt acaactatta
     3721 taaatattat tgctcctatt agcaactgtg tatgcataat aaccattcct ttatttaaaa
     3781 tgcttctgta tacccttgta atgccttata cccacgagta atgttcataa ctcacacaaa
     3841 tcagccattt actggctaaa tccctgtttg tgtcacctta acctgtacaa gctgttcatt
     3901 agtacatttt ttatggtata atcaattaat ctcatttaaa aaggggtgta aaaaaatgtt
     3961 tcagaacaat actgaagatg ttgcaaagca gaaattgctc ctgctctata tcctgaaggt
     4021 atcgcctgaa gctctgacaa aagagaagct gtcagaaatt ctaatagaca atttcaatat
     4081 gaatttcttt ataatccaac aatacctttc cgagctta
//
LOCUS       DASRQF010000322         2938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_313629, whole genome shotgun sequence.
ACCESSION   DASRQF010000322 DASRQF010000000
VERSION     DASRQF010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313629"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1448
                     /gene="gpmI"
                     /locus_tag="VFD79_08535"
     CDS             <1..1448
                     /gene="gpmI"
                     /locus_tag="VFD79_08535"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HZH94137.1"
                     /translation="KPNFDRIISKYPNTTLRASGLDVGLPEGQMGNSEVGHLNIGAGR
                     VVYQELTRITKEIRDGGFFRKQEFLDAIENAKDRNSSIHLMGLVSDGGVHSHNTHLYA
                     LLELMKQHKMEKVFVHVILDGRDVPPTVGSQHVQELQDKIKEIGVGKIASVAGRYYTM
                     DRDKRWERTQLGYDAMVLGEGEKASDPVKAVEESYKRDINDEFMLPTVIEEDGRPVST
                     IDDGDSLIFFNFRPDRARQITRAIVDEDFDGFKRKKKVATYYVTMTEYDKTIKNVHVA
                     YTNEGLHNTLGEYVSNKGKTQLRIAETEKYAHVTFFFNGGREEPFEGEDRVLVPSPKV
                     ATYDLQPEMSAYQVKDQVLEKVKSGKYDLIILNFANPDMVGHTGVIPAAVKAVETVDQ
                     CMGEIVKLMEEVGGRILVTADHGNAESMLDRESGGTVTAHTSNKVPLILVGETDVTLR
                     EGILADLAPTILDLMKLEKPDEMTGRSLIEK"
     gene            1470..2762
                     /gene="eno"
                     /locus_tag="VFD79_08540"
     CDS             1470..2762
                     /gene="eno"
                     /locus_tag="VFD79_08540"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HZH94138.1"
                     /translation="MSLITDIYAREVLDSRGNPTVEVEVWTELEAFGRAIVPSGASTG
                     IYEAVELRDMDKSRYLGKGVLKAVENVNDIIADEIIGMEVFDQIMVDKVLMEIDGTEN
                     KGKLGANAILAVSMAVARAAANELGMPLYQYIGGVNGKTLPVPMMNILNGGDHADNNV
                     DIQEFMVMPVGAEGFREGLRMGTEIFHNLKAVLKAKGMSTSVGDEGGFAPNLSSNEEA
                     LVTIVEAIEKAGYKPGEDVVLALDVAASGMYDEDKKVYNLKGEGKVMTAEEMVDYYGD
                     LCDKYPIISIEDGLGEDDWDGWKLMTEKLGKKIQIVGDDLFVTNTERLQRGIDMGVAN
                     SILIKLNQIGTITETLDAIELAKTNGYTAVISHRSGETEDSTIADLAVATNAGQIKTG
                     APSRTDRVAKYNQLVRIEDGLGEMSEYKGIKTFYNLKK"
BASE COUNT          979 a          496 c          766 g          697 t
ORIGIN      
        1 agaagcctaa ttttgaccga attatatcca agtatccaaa tacaacctta agggcaagcg
       61 gtttggatgt tggcttgcct gaaggacaga tgggaaactc agaggtgggc catctcaata
      121 tcggtgccgg aagagtcgta tatcaggaat tgaccagaat taccaaggaa atcagagatg
      181 gaggcttctt cagaaaacag gaatttctgg atgccataga aaatgcaaag gacagaaact
      241 catccataca cctgatgggc ctggtatccg atggcggtgt tcacagccat aatactcacc
      301 tctatgccct gcttgagctt atgaaacagc acaagatgga gaaggtgttt gtacacgtca
      361 tactcgatgg aagggacgtg cccccaacag ttggaagcca gcatgtacag gagcttcagg
      421 ataagatcaa ggagataggt gtaggtaaaa tagcgagtgt tgcaggaaga tactacacaa
      481 tggacaggga taaaaggtgg gaaagaaccc agcttggata tgatgccatg gtccttggag
      541 aaggtgaaaa ggcttcagat cctgtaaagg cagtggaaga atcatacaag cgggacataa
      601 acgatgagtt tatgcttcca actgtaattg aagaagatgg tagaccagtt tcaacaattg
      661 atgatggaga ttcgctcata ttcttcaatt tcagaccaga cagggccaga cagataacaa
      721 gggctattgt tgacgaggat tttgatggct tcaaaagaaa gaaaaaggta gccacttact
      781 acgtaaccat gacggagtac gacaaaacca taaagaacgt tcatgtggct tatacaaacg
      841 aaggtcttca caacaccctt ggagaatatg taagcaataa aggaaagacc cagctaagga
      901 ttgcagagac tgaaaaatat gcacatgtga ccttcttctt caatggagga agggaggaac
      961 cttttgaggg tgaggatagg gttcttgtac catcgccaaa ggttgcaacc tatgacctgc
     1021 agcctgaaat gtccgcatac caggtaaagg atcaggttct tgagaaagtt aaatcaggga
     1081 aatacgacct gataatcctc aactttgcaa atcctgacat ggttggtcat acaggcgtaa
     1141 tccctgctgc tgtaaaggcg gttgaaactg tagaccagtg tatgggtgaa atcgttaagc
     1201 ttatggagga ggttggtggc aggattctag taaccgccga ccatggaaat gcagaatcca
     1261 tgcttgatag ggaatcaggt ggaactgtta cagcacacac aagcaacaag gttcctctca
     1321 tacttgtcgg ggagacagat gtcaccctta gggagggtat tcttgccgat ttggctccaa
     1381 ctatactgga cctgatgaag cttgaaaagc ctgatgaaat gactggaaga tccttgattg
     1441 aaaaataata ttagaatgga gtgaaatgaa tgagtttaat taccgatatt tacgcaagag
     1501 aggtacttga ttcaaggggc aacccaacgg ttgaggtgga agtttggaca gagcttgaag
     1561 catttggaag agccatagtt ccatcaggtg cctcgacagg catatatgag gcagttgagt
     1621 tgagggatat ggacaagtca agatatttgg gaaaaggcgt gttaaaggcg gttgaaaacg
     1681 taaatgacat aattgccgat gaaataatcg gaatggaagt ttttgaccaa atcatggtag
     1741 acaaggtgct catggaaata gatggaacag agaacaaggg caagttgggg gcaaatgcaa
     1801 tccttgcagt atcaatggct gttgcaagag ctgcggcaaa tgaacttgga atgcccctct
     1861 accagtatat tggtggagta aacggaaaaa ccctcccggt cccaatgatg aatattctta
     1921 acggaggaga ccatgctgat aacaacgttg acatccagga gttcatggtt atgcctgttg
     1981 gagcagaagg attcagggaa ggtcttagaa tgggtacaga aatattccat aacctaaagg
     2041 ctgtgctaaa agccaaggga atgagcacat ccgtaggaga tgagggtggt tttgcaccaa
     2101 acctttcaag caacgaggaa gcacttgtaa caattgtgga agcaattgaa aaggcaggct
     2161 ataagccagg tgaagatgta gttcttgctt tggatgtggc agcaagtggt atgtatgatg
     2221 aagacaagaa ggtctataac cttaagggtg agggaaaggt tatgaccgct gaggaaatgg
     2281 ttgactacta cggagatctt tgtgacaagt accccataat ctcaattgag gatggacttg
     2341 gagaagatga ttgggatggt tggaaactga tgactgagaa acttggaaag aaaattcaga
     2401 ttgtgggaga tgacctgttt gtgacaaaca ctgaaagact gcaaagagga attgacatgg
     2461 gggttgcaaa ttcaatcctt ataaagctta accagatagg tacaatcaca gagaccctgg
     2521 atgcaattga gcttgcaaaa accaatggat atactgcagt aatttcacat aggtcaggtg
     2581 agacagagga ttctacaata gcagaccttg cagtagctac aaatgcagga cagataaaga
     2641 caggcgcacc ttcaaggaca gacagggttg ctaaatacaa ccagctggtc agaattgagg
     2701 atggacttgg tgagatgtct gaatacaagg gtattaaaac attctacaac cttaagaaat
     2761 aggttgttga caagcagaat attaaagagt agaataaaac gcatatgcaa actatttgca
     2821 tttgcgtttt atttttttgt ctttgggtaa aataggaagg caaagtaaaa catcagtcta
     2881 aaacttgttg gaaattggag gttaataatg aaaagaagag gaattttatt tttaataa
//
LOCUS       DASRQF010000323         6254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1561965, whole genome shotgun sequence.
ACCESSION   DASRQF010000323 DASRQF010000000
VERSION     DASRQF010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6254
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561965"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(336..1052)
                     /locus_tag="VFD79_08545"
     CDS             complement(336..1052)
                     /locus_tag="VFD79_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH94139.1"
                     /translation="MGRYGYPKQASAAEKKAKATKALEKLRKKNPDIEPVIIEGRALV
                     KTWWGKAWNQNLESYADYSNRIARGRSYVKNNAVLDLKIGEGVVVALVQGSGSKQYSV
                     RIEIDTLSDEKWRQVTEICNNRIENLEQLIEGKFPEELEVLFKERKYGLFPSPSEIRF
                     GCSCPDWAVMCKHVAAVLYGVGARLDHDPMLFFTLRDIDGKELVRKSMESKLDNMLKN
                     AGNKSDRQIDIREAEEIFDL"
     gene            complement(1052..3730)
                     /locus_tag="VFD79_08550"
     CDS             complement(1052..3730)
                     /locus_tag="VFD79_08550"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZH94140.1"
                     /translation="MAIKNTKMHMALNAYFVPGGFVIEETPEEILSSGKECEDLLKEW
                     IIRFDKDKYQALYHLGFSEKRSWFTPSIEYLYKISDMVIQKLLRYPGIEYGRELVIDE
                     ITLSPEEIDSLIEKVPFVVGGEHVDHYWIIHIFNNLLTVFSNEIRAYEGTVARYFSEL
                     NSNMNVAGRVFFHLVENKDSDYPFAFMATYSTKAEKSRKALHTPLKNALSEYKGDEKL
                     LLSLMSTVIKAADSSQLISDLMESGELFSPLKFTADEAYTFLKEIHIYEGAGIMCRVP
                     DWWRKKRNSFQLSVSIGEKEPSRVGLDAIMDFSPSLKVGEEDLTEKELREFLAMAEGL
                     VQYKGKWVEINKEKLKAVLEAFERLKDISGDSVISLGDAMRMELNIDKVMDMPSEDVE
                     ISVSNGQWLRMLRETLVKPKAYNLVEGSDSFKGDLREYQEIGFQWLNKMSQFGFGACL
                     ADDMGLGKTVQVIAFLEHSRVDHGGRALLVIPASLIGNWQMEVEKFAPEMEYKVLHKS
                     AGNGFEGLDEEDSTFLYITTYGMVSRLDEIRERTWDYLILDEAQAIKNPRTKQTRSVK
                     EINARVRIALTGTPIENRLGDLWSLFDFLNQGLLGTAKEFSGFTKQLYEDGGGYEKLR
                     KMIQPFILRRLKTDKSIISDLPEKIEINEYSILSKKQIALYRQLVDKIAIGLDSAEGI
                     ERKGLILSSIMRFKQICNHPDQYMGREEFKPENSGKFQLLREICETIHEKRERVLVFT
                     QFREMTGPISDFLEEIFQKEGLVLHGGTPVKKRNEMVERFNGDHYVPFMVLSLKAGGV
                     GLNLTAANHVVHFDRWWNPAVENQATDRAFRIGQSKNVMVHKFISKGTIEEKIDAMIA
                     EKQKLAGDILSSTGEQWITEYDNEQLMKLFLLGGED"
     gene            complement(3860..4102)
                     /locus_tag="VFD79_08555"
     CDS             complement(3860..4102)
                     /locus_tag="VFD79_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH94141.1"
                     /translation="MGGRKKQKKKNKDKEVEIWTEEQYEDYLKGVYGLDFIAGYTEGG
                     MPFGLPADETAEEKIIETENVERKTGACCDDDDLPF"
     gene            complement(4109..4684)
                     /locus_tag="VFD79_08560"
     CDS             complement(4109..4684)
                     /locus_tag="VFD79_08560"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZH94142.1"
                     /translation="MEFGRKLYSLGEIYTILYKGIGSAPHMTKAKRKGEMTSEFIERI
                     MLAVTEVNGCPLCSYAHTKMALEAGMNNEEIHNMLSGVMEDVPEEDLQAVMFAQHYAD
                     SRGYPSRESWDRVVENYGIVKAKGILGAIRAIMMGNALGIPWGSFFNRFKGQPDERSN
                     LGYELGVMVIGSIMIPVALVHALLASMKTRS"
     gene            complement(4760..4963)
                     /locus_tag="VFD79_08565"
     CDS             complement(4760..4963)
                     /locus_tag="VFD79_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH94143.1"
                     /translation="MKIGFRKPSLNKRIRSRTSIKRQIIHRAGLKMPRGYGWARNPKK
                     YVYNKVYNRTSFDIFKSLKKLLK"
     gene            complement(4989..6098)
                     /locus_tag="VFD79_08570"
     CDS             complement(4989..6098)
                     /locus_tag="VFD79_08570"
                     /inference="COORDINATES: protein motif:HMM:NF025298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HZH94144.1"
                     /translation="MKKIIVLLLVFVLILGSGVFAEDIAVEVDGRKLDFDVAPTIIDG
                     RTILPARTIFESLGLEVKWDDVTRTVTGIGEGTELSLVIGQSVAYVNGEPVNLDVAPK
                     IIDDRSMVPVRFVAESTGADVDWDNSTRTVLIRSETNLKEDPFGDNIYIEVDGGDLSG
                     HRLPNVVVDIGYGDREYWAYTNEYGQLVRVVADEIVLQDESTEDVLHSGRYFHDEAKV
                     PGTEHPELDEGHVIADSLGGVSNAYNITPQNSTLNRHGDQAYMEKTIREAGGATNFEA
                     IITYPNVNTQIPSHYKYTYTVWGSEVVDEFDNINPDDYSSNTKIFNLGLEDLLGVDMD
                     LPHIFNGLDLESTVDGIRIKGKQLLEAIMSNTRLP"
BASE COUNT         1590 a         1657 c          992 g         2015 t
ORIGIN      
        1 gttcaaccaa tctatcctgt ataagtgctg cttccgacgt tatatttgtc ttggtttgaa
       61 atagtgtatc attgctcatt aaaagtgctc caacaattac aagtgcaagt atcaaaacta
      121 ctataatcat tgtcctaaat actattttca ttgttcattt cacctcacta atattcctcc
      181 aattcggtgt ctatgtttat cttttcaagt ttatgattta ccctcgtgaa aagtggaatt
      241 ccttcaccta gaaatgaata tccttactcc aatgtcatac taaatttact ttaaatagct
      301 catatccaag ctgcagattg ccccttactg acaatctata aatcaaatat ctcctctgct
      361 tccctaatat ctatttgcct atcgcttttg ttcccagcat tctttagcat attatccaac
      421 ttgctttcca ttgattttct gacaagctct ttaccatcaa tatccctcaa ggtaaagaaa
      481 agcattgggt catgatcaag tcttgcaccc actccataca aaacagctgc aacatgctta
      541 cacattacag cccagtcggg acagctgcat ccaaaccgga tttcactagg ggaagggaat
      601 aatccatatt tcctttcctt aaacagaacc tccaactcct ctggaaactt gccttctatc
      661 aactgttcaa gattctcgat tctattgttg cagatttctg tgacctgcct ccacttttca
      721 tcactaaggg tatcaatctc tattcttaca gaatactgct ttgagccact tccctgaaca
      781 agtgccacca ccaccccttc acctatctta aggtcaagaa cagcgttatt cttcacatag
      841 cttctccctc tggcaatcct gttgctgtaa tcagcgtagc tttcaagatt ctggttccag
      901 gccttacccc accaagtctt taccaaggcc cttccttcta tgattacagg ttcaatgtca
      961 ggattctttt ttctcagttt ctccagagcc ttggttgcct tggctttttt ctcggctgca
     1021 gaagcctgtt tggggtatcc ataccttccc atcaatcttc acctcccagt agaaacagct
     1081 tcatcagctg ctcattgtca tactcagtta tccactgctc tccagttgag ctcaatatgt
     1141 cccccgcaag tttttgcttc tcagcaatca tagcgtctat cttctcctct atggtccctt
     1201 tgcttataaa tttatggacc ataacattct tactctgtcc aattcggaat gccctgtccg
     1261 tagcctggtt ctcaacggct ggattccacc atctgtcaaa atgcacaacg tgattggctg
     1321 cagtaaggtt aagcccaaca cccccagcct tgagagaaag taccataaat ggaacatagt
     1381 ggtccccatt gaacctttca accatttcat tgcgtttttt cactggtgta ccaccatgga
     1441 gcaccaggcc ttccttttga aaaatctcct ccaggaaatc tgaaatgggg ccggtcattt
     1501 ccctgaactg agtgaaaaca agcaccctct ctcttttttc atgtattgtc tcacaaatct
     1561 cccttaaaag ctgaaatttt ccgctattct caggtttgaa ctcctccctg cccatgtact
     1621 ggtcaggatg gttgcatatc tgtttgaatc tcattatgct cgatagtatt agtccctttc
     1681 tctcaattcc ttcggcgcta tccagaccaa tggcaatctt gtctaccaat tgcctataca
     1741 aagcaatctg cttctttgat agtattgaat actcatttat ctcaatcttc tcaggaaggt
     1801 cggatattat gctcttgtcc gtcttcaatc ttctaaggat aaaaggttgg atcatcttcc
     1861 tcagcttctc atagcctcct ccatcctcat aaagctgctt tgtgaatcct gaaaactcct
     1921 tggcagtgcc taatagtccc tggtttagaa agtcaaacag tgaccacagg tctcccaatc
     1981 tgttctctat tggtgtccca gtcaatgcta tcctcactct agcgtttatt tccttgactg
     2041 acctggtttg tttggttctg ggatttttta ttgcctgggc ctcatcaagt atcaggtagt
     2101 cccaggttcg ctccctgatt tcatccaatc ttgaaaccat cccatatgtt gttatataca
     2161 ggaaagtaga atcctcctca tccaatccct caaacccatt tcctgcgctc ttatgtagta
     2221 ccttatactc catttccggg gcaaatttct ccacttccat ctgccagtta cctatcaggg
     2281 atgcaggtat aacaagaagt gccctgcctc catggtccac tcttgaatgt tcaagaaaag
     2341 ctatgacttg aacggtcttt ccaagtccca tatcatcagc aagacatgca ccaaatccaa
     2401 attgtgacat cttgtttagc cattggaaac ctatttcctg atattccctc aggtcccctt
     2461 taaatgagtc cgacccttcc accaagttgt atgcttttgg tttgaccaag gtttccctaa
     2521 gcatcctgag ccattgacca ttgcttacac ttatctcaac atcttcactt ggcatgtcca
     2581 ttaccttgtc aatgttaagc tccatacgca tggcatctcc aagggagata acactatcac
     2641 cggaaatgtc cttaagcctt tcaaaggctt ccaacacagc ttttagcttt tccttgttta
     2701 tttccaccca tttccccttg tattggacca acccttctgc catggcaaga aactcacgaa
     2761 gctctttttc tgtcaaatcc tcctctccca ccttcaagga tggggaaaag tccattattg
     2821 catccaatcc aaccctggat ggttcctttt cacctattga aactgaaagc tggaatgaat
     2881 tcctcttctt cctccaccaa tctggaaccc tgcacattat cccagcacct tcatagatat
     2941 ggatttcctt aaggaaggtg taggcctcat cggctgtaaa cttcaacggg gagaacagtt
     3001 ctccgctttc catcaggtcc gatatcaatt gactactgtc tgcagccttg ataacggtgg
     3061 acataagtga aagcagcagc ttctcgtctc ctttgtactc agaaagggca tttttcagag
     3121 gtgtgtgcag tgcctttctg ctcttctcag ccttagtcga gtatgttgcc ataaaagcaa
     3181 agggatagtc actgtccttg ttttcaacca gatgaaagaa taccctccct gcaacattca
     3241 tgtttgaatt cagttccgaa aaatatcttg caacagtccc ctcatatgct ctaatctcat
     3301 tgctaaaaac tgtcaacagg ttattgaaaa tatggataat ccaatagtgg tccacatgtt
     3361 ccccaccaac tacaaagggt accttctcaa tcaaggaatc aatctcctct ggactcaggg
     3421 ttatttcatc tatcaccagt tctctgccat actcaatccc gggatatctt aagagttttt
     3481 gaatgaccat atcggaaatt ttataaaggt attcaattga tggagtaaac cagctccttt
     3541 tctcagaaaa tcccaaatga tacagggctt gatacttatc cttatcaaac ctaataatcc
     3601 attccttcag caggtcctca cactccttgc ccgaagacag tatctcttct ggtgtttcct
     3661 ctataacaaa acctccaggt acgaagtatg cattcaacgc catatgcatt tttgtatttt
     3721 tgattgccat tgccttttcc tccaagtcgt ttgagtctat ctatgactct aatttaaaca
     3781 ctggaaacag atatgtctag taattgttcc taaaagaatt acatactgta tgtgatcccc
     3841 gaaaaacctg ccctcacaat tagaatggta aatcgtcgtc atcacagcaa gctcctgttt
     3901 tcctttcaac attttccgtt tcaataatct tctcctcggc agtctcatct gcaggtaatc
     3961 cgaaaggcat gcctccctct gtataaccgg caatgaaatc cagcccatat acccccttaa
     4021 gataatcttc gtattgctct tctgtccata tctcaacttc cttgtcctta tttttcttct
     4081 tttgcttctt tcttccaccc aaattccttc aactccttgt tttcattgaa gcaagtagcg
     4141 catgcaccaa agccacagga atcatgatgc ttccaattac cattactcca agctcatagc
     4201 caagattgct tctttcatca ggctgtcctt taaatctatt gaaaaacgat ccccagggaa
     4261 ttccaagtgc atttcccatc attattgccc ttattgctcc aaggattccc ttggccttaa
     4321 caattccata gttctcaaca accctgtccc aagactccct ggaagggtag cctctggaat
     4381 ctgcataatg ctgtgcaaac attacagcct gcaggtcttc ctcaggaaca tcctccatca
     4441 caccggagag catgttgtga atctcctcgt tgttcatccc agcctcaagg gccatcttgg
     4501 tgtgtgcata ggaacacaag gggcacccgt tgacctcggt tacagcaagc atgatcctct
     4561 ctataaattc actggtcatc tcacccttac gctttgcttt tgtcatatgt ggggctgaac
     4621 caattccctt gtacagtatc gtgtaaattt ctcccaatga gtataatttt cttccaaatt
     4681 ccattttatc ccttcatttc tgttttgttt tttttgagta ttgatgattg ttgatcggga
     4741 attattaaat agtatccttc tacttaagga gcttctttaa agatttgaaa atatcaaacg
     4801 acgtcctgtt gtataccttg ttgtaaacat acttctttgg attcctggcc catccgtatc
     4861 ctcgtggcat tttcaggcca gctctgtgga tgatctgtct ttttatactt gtcctggatc
     4921 ttattctttt gttcaagcta ggctttctaa atcctatttt caactttaca acctcctatc
     4981 tcctcttcct aaggcagcct tgtattggac attatggctt caagtagttg cttacccttg
     5041 atccttatgc catcaactgt actctccagg tccaacccat tgaatatatg tggcaggtcc
     5101 atatccactc caagcagatc ctcaaggcca agattaaata tctttgtatt ggagctgtaa
     5161 tcatccggat tgatattgtc aaactcgtcg actacttcac tcccccagac ggtgtaggtg
     5221 tacttataat ggcttggtat ctgtgtattc acatttggat aggtaattat ggcctcaaaa
     5281 ttggtggctc caccagcttc ccttatggtc ttctccatat aggcttgatc accatgtctg
     5341 ttaagtgtac tattctgagg agttatgttg tatgcatttg atactccccc aagtgaatct
     5401 gcaattacat ggccttcgtc aagctcagga tgctctgtac ccggcacctt tgcctcatca
     5461 tggaagtatc ttcctgaatg gagcacatcc tccgtgctct catcctggag cacaatctca
     5521 tctgcaacaa cccttaccag ctgaccatac tcatttgtat atgcccagta ttccctatca
     5581 ccatatccaa tgtcaactac cacatttgga agcctgtgcc ctgataggtc tccaccgtcg
     5641 acctctatgt agatgttgtc accaaatgga tcttccttaa gattggtctc acttcttatg
     5701 agaactgtcc ttgtggaatt atcccaatcc acatccgccc ctgttgactc agccacaaat
     5761 cttacaggaa ccatgcttct atcatcaatt atttttggag ctacatcaag atttacaggc
     5821 tctccattta cataggcaac actttgtcct atgacaagtg aaagctcagt tccctctcca
     5881 atcccagtca cagtccttgt cacatcatcc catttaactt caagtcccaa tgactcgaag
     5941 attgtccttg ccggcaggat tgtcctccca tcgattatgg taggagcaac atcaaaatct
     6001 agctttctcc cgtcgacctc aacggctata tcctctgcaa atacaccgct tccaaggatc
     6061 agtacaaata ccagcaacaa aactataatt ttcttcataa agctatacct ctgatttctt
     6121 tgttttctgt catcctacag gaacaaacta tcaacctgtt ccttttgcat caacactcca
     6181 atgagtattg gtcataacct tagatataac cagtatatca tcttccatat tctcaaacaa
     6241 taaatcccaa atca
//
LOCUS       DASRQF010000324         2843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_918471, whole genome shotgun sequence.
ACCESSION   DASRQF010000324 DASRQF010000000
VERSION     DASRQF010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918471"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VFD79_08575"
     CDS             <1..743
                     /locus_tag="VFD79_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004837011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HZH94145.1"
                     /translation="ELKENPDMERVPKTFIFGAKAAPGYARAKAIIRFINNIAEMVNN
                     DPATEGKLKVIFVQNYRVSYGEKLFPAADLSEQISTAGKEASGTGNMKFMLNGAPTIG
                     TYDGANIEIVEEAGEENNYIFGLRVEDIEKIRDSYNPEEIYHNNPKLRRVMDTLVDGT
                     FQDTDGSYRELHNSLLYGADWHKADNYFILKDFEEYRKAHEKADLDYKDRLGWARKCW
                     LNMANSGKFSSDRTIEQYAQEIWEINKI"
     gene            869..2209
                     /locus_tag="VFD79_08580"
     CDS             869..2209
                     /locus_tag="VFD79_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012803600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HZH94146.1"
                     /translation="MKMKKSLFLVMFLVLALAITACAPGAEEEPAPSEQPTGETPAEG
                     ISATISVQVEADWREYYEQAVARVIENHPDATIELIETGSFDHLDVLDATDVTNEDVA
                     DVFAIPADRIYGLSNNEALAPMDAMAMADNVGGFGNYDEGLGGNFNIDGDYLAFPMNI
                     ETLVNFVNTANAEVKGIDLESEIEFTELGHVDMLIPAFDAWFGVSFANAGGIELLGHD
                     ESGQLYSDMTKDFSDLNEQQQAVFTALYNYWDAHYSAGTPLWDENAAWGYMDTEFSTG
                     GQNVIRLEGPWSTGSLSALAGDGEDLDIIPINQVLVEGYPLAHWKGGWGLAINSRNEM
                     DADKMALSQAMIEEIMNTDYAIDFFKATGKIMENVSVDEYLSSDLSETDKKVISAVLE
                     SYDAAPARPLFTEWGSVWDTWKNGLLSWSSVNPGSVEEAYDAMKASFEAMMMNY"
     gene            2276..2731
                     /locus_tag="VFD79_08585"
     CDS             2276..2731
                     /locus_tag="VFD79_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH94147.1"
                     /translation="MSEKNHLSLNKFYIILSLTSILVLLASVEALLLVKDGDIFLKWA
                     RENFPDNAPDNILFTQYVSSNLAMYFLKVIIPSTIAVMAFITHTKAEFSHIVVYIWVI
                     LSFGGLAYSTADMNFQSAFFYLNGIIYTGLILFIYFGVGISRDNTGGEN"
BASE COUNT          899 a          543 c          672 g          729 t
ORIGIN      
        1 atgagttgaa ggaaaacccc gatatggaaa gggtgcctaa gaccttcata ttcggtgcca
       61 aggctgcacc aggttatgcg agggcaaagg ccatcataag attcataaac aacattgctg
      121 aaatggtcaa caatgaccct gcaacagaag gtaagctcaa ggttatattt gtgcagaact
      181 atagggtgtc ctatggcgaa aaactcttcc ctgcagcaga cctgtcagaa cagatttcaa
      241 ctgccggtaa ggaggcgtcc ggtactggta acatgaaatt catgctcaat ggagcgccga
      301 ccataggaac ctatgatggg gcaaatattg agatagttga agaggctgga gaagaaaaca
      361 actatatatt tggattgagg gtggaggaca tagagaaaat cagagactca tacaatccag
      421 aggagattta ccacaataat cccaagctta ggagagttat ggatacactg gttgatggga
      481 cattccaaga tacggatggg tcatataggg agcttcacaa ttcacttctt tacggagctg
      541 attggcacaa ggctgacaac tatttcatcc tcaaggactt tgaggaatac aggaaagccc
      601 acgaaaaagc agatcttgac tacaaagata gattgggttg ggcaagaaaa tgttggttaa
      661 atatggcaaa ttcgggtaaa tttagttcag acagaactat tgagcaatat gcccaggaaa
      721 tttgggaaat caataaaatc taggtcaagg ggcccagccc cttgtccaca ttaagttgga
      781 tttaatttct ccggaaacgg agagttaaat tatatatttt tgcgttcatg caagcgtatg
      841 cacaaaaaag attaggggga agaaaaacat gaaaatgaaa aagagtttat tcttggtaat
      901 gttccttgta ctggcacttg ccattacggc atgtgcacca ggagcagagg aagagcctgc
      961 accatcagag caaccaaccg gtgagacacc tgctgaaggc atttcagcta ccatatcagt
     1021 tcaggttgaa gctgattgga gagagtatta cgagcaggcg gtagcaagag tgatagaaaa
     1081 ccacccagat gctacaattg agctaattga aactggttca tttgaccatc ttgacgtact
     1141 tgatgcaact gatgttacca atgaggacgt agctgacgtg tttgccattc ctgcagacag
     1201 gatctacgga ctttccaaca acgaagcact tgcaccaatg gatgcaatgg caatggcaga
     1261 taatgtaggt gggttcggca actatgacga aggactaggc ggaaacttca atattgatgg
     1321 ggattatctg gcattcccaa tgaacattga gacccttgtt aattttgtaa acacagctaa
     1381 tgctgaggtt aagggaatcg accttgaaag tgaaatagag tttactgagc ttggtcatgt
     1441 ggatatgctg atcccggcct ttgatgcgtg gtttggggtt tcatttgcaa acgctggagg
     1501 aattgagctt cttggacacg acgagtcagg tcaattgtat tcagatatga ccaaggactt
     1561 ctctgaccta aatgaacagc aacaggctgt tttcacagcc ctatacaact actgggatgc
     1621 acattattca gctggaacac ccctttggga tgaaaatgca gcctggggtt acatggacac
     1681 cgagttttca actggtggac agaatgtaat aagacttgaa ggtccatggt caacaggaag
     1741 tctctcagca cttgctggag atggagaaga ccttgatatc ataccaataa accaggttct
     1801 ggttgaggga tatcctcttg cacactggaa aggtggctgg ggactggcaa taaactcaag
     1861 aaatgagatg gatgcagata agatggccct atcacaggca atgattgagg aaatcatgaa
     1921 cacagactac gccatcgatt tctttaaggc gacaggcaag atcatggaga acgtatcagt
     1981 tgatgaatac ttgagttcag acctttcaga aactgacaag aaggtaatct ccgcagtact
     2041 ggaatcctac gatgcagctc ctgcaagacc tcttttcaca gagtggggat ccgtatggga
     2101 tacctggaaa aacggacttc tatcatggtc atcagtaaat cctggatcag ttgaagaggc
     2161 ttacgatgca atgaaagctt catttgaagc catgatgatg aactactaaa taattttggg
     2221 aatagagctt aggctctatt cccaaaatat ttatccatag ggcaagggga aaataatgag
     2281 tgaaaaaaat catttatcgt taaataaatt ttacataata ttatcattaa ccagtatact
     2341 ggtactgctt gcttctgttg aagccctcct gcttgtcaag gatggtgaca tcttcctaaa
     2401 gtgggcaagg gaaaactttc cggacaatgc cccggacaat atattattca cccaatatgt
     2461 atcctcaaac cttgccatgt acttcctaaa ggtaatcatc ccttcaacaa ttgcagttat
     2521 ggcgtttata acccacacaa aggctgaatt ctcacatatt gtggtctata tctgggtaat
     2581 attatccttt ggaggcttgg cctattcaac cgcagacatg aactttcaat cggccttctt
     2641 ttatttgaac ggaataatct atacgggact catacttttt atttattttg gagtgggtat
     2701 ttcaagagat aacaccgggg gtgagaatta gatggcaacc tacagcttgt atttaaagga
     2761 cgacattgga aacaagtatg aaagaaaaaa caggtatctg cttcagcttg cccagctgga
     2821 cgagaaaatc aagcatgaaa ctg
//
LOCUS       DASRQF010000325         2936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_314083, whole genome shotgun sequence.
ACCESSION   DASRQF010000325 DASRQF010000000
VERSION     DASRQF010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314083"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VFD79_08590"
     CDS             <1..292
                     /locus_tag="VFD79_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019108465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HZH94148.1"
                     /translation="AADQYLAIVIPGRMYKDAYDEAGLEPRLLSRTLEDGGTLTSALI
                     PWNTCGAYMQSVLLVSPLAYAPFAFMNLINPIVAIIYGFTGFSIKKAEPKAE"
     gene            complement(497..1471)
                     /locus_tag="VFD79_08595"
     CDS             complement(497..1471)
                     /locus_tag="VFD79_08595"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HZH94149.1"
                     /translation="MVKPKVLIGYPIKDEIMEYVGEHCELVQMDYSKAITYDDIYERI
                     DDVEGAVVLITPIDKKLLERGKKLKVVSNITVGYNNFDIEEMKKAGVIGTHSPGILDD
                     TVADFVMGLLISTARRITELNSFVKGGSWKKIENNHLMGLDVSGTRVGIIGMGRIGEE
                     VARRCSLGFKMDVSYHNMSRKEGAEEEFGISYKPMDQLLAESDFVIILTPLTEETHHL
                     MGEREFSLMKKNAILINASRGPVVDEKALIKALQEERIAGAGLDVYEKEPMDGDNPLL
                     KMDNVVLSPHIAAGTQRTYDDLAWNAARCMVRILEGEEPVNVIPEMRG"
     gene            complement(1484..2458)
                     /locus_tag="VFD79_08600"
     CDS             complement(1484..2458)
                     /locus_tag="VFD79_08600"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HZH94150.1"
                     /translation="MKRPKIFIANEIPKSVEEYIGDHCDYVMWDFSKRKTFQGIVDHI
                     GDVDGILEVGMKIDKELLKHAPKLKVVSNISVGYNNYNLDDMKDMGVLGTNTPGVLDN
                     TVADLIMGLIISAGRRIVEADADTKAGNWQKGEMDKFFGKDIHHSTLGIIGMGRIGEA
                     VAKRAQGFDMEIVYHNRKRKPQAEERFQAEYMEMDQVLQNADFLVVMTPLSEETHHLI
                     GEVELGKMKEDAFLINASRGPVVDEEALIKALQEGWIAGAGLDVYEREPIDRKNPLLQ
                     MRNVVTLPHIGSATRKTRDEMAMVAAKAIVEVLEKGDSPYIVPELRKR"
     gene            complement(2479..>2936)
                     /locus_tag="VFD79_08605"
     CDS             complement(2479..>2936)
                     /locus_tag="VFD79_08605"
                     /inference="COORDINATES: protein motif:HMM:NF016083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HZH94151.1"
                     /translation="KAYNSINYNTIILVTSILILKDIILQMDDMLQMINKMLMGVEGG
                     LGVMVIFTLVSFFFGYITGYPTSSLAITLPLLSMMDLGSNALHIYTFFLVAVSFVGYY
                     YSPLHLCQVLTIGEMKVTTGDLYKEYLPYFALQMLLIFATTLGLMAIFA"
BASE COUNT          736 a          774 c          575 g          851 t
ORIGIN      
        1 ggcagctgac cagtacctgg caatcgtaat acctggtaga atgtacaagg atgcatacga
       61 tgaagctgga cttgagccaa gacttctttc aagaaccttg gaggatggag gtacattgac
      121 ctcagcactt attccatgga acacctgtgg agcctacatg cagagtgtac ttcttgtatc
      181 accacttgca tatgcaccat ttgccttcat gaacctgata aatccgatag tagcaatcat
      241 ctacggattc acaggcttca gcatcaagaa ggcagagcca aaagcggagt agtaaaaatc
      301 cctgacattt aaaatgtcag ggatttttta attcacaatt cacaattcac agtgcacaat
      361 tttaaagcaa gaactagggt ctaagactcc tcgccttact cagagtgaca ataatgtttt
      421 cctgagtgta gcgaaggatt ttagaccctt gagattgacc ctattcaatt gtgaattgtg
      481 atctgtgaat tgtgaattat cctctcattt ccggaatcac attcaccggc tcctcaccct
      541 caagaatcct taccatgcac ctggcagcgt tccaggccaa atcgtcatag gtcctctggg
      601 tgcctgcggc aatatgtggc gaaagaacca cattgtccat cttaaggagg ggattgtccc
      661 catccatggg ctccttctca tatacatcaa gtccagcccc agcaatcctt tcttcctgaa
      721 gtgccttgat cagggccttt tcatccacaa ccggtcccct tgatgcgttt atcaggattg
      781 catttttctt cataagggag aactcccttt cccccataag atggtgggtc tcctccgtaa
      841 ggggtgtaag aataatcaca aaatcacttt cagcaagcag ctggtccatg ggcttgtagg
      901 aaattccaaa ttcctcctcc gccccttcct tcctggacat gttatggtag gacacgtcca
      961 tcttaaagcc aaggctgcac cgtctggcaa cctcctcccc aattctaccc attccaatta
     1021 taccaaccct ggtcccggaa acatccaggc ccattaagtg gttgttctcg atttttttcc
     1081 agctaccacc ctttacaaag gagttaagct cagtgattct tctggccgtc gaaataagca
     1141 gtcccatgac aaaatcagcc actgtatcat caagtatacc aggtgaatgg gttccaatca
     1201 ccccagcctt cttcatctcc tctatgtcga agttattgta gcccactgta atgttgctga
     1261 caaccttaag cttcttgccc ctctccagga gcttcttgtc aattggggtt attaggacca
     1321 cggctccctc aacatcatcg atcctctcgt aaatgtcatc ataggttatt gccttggaat
     1381 agtccatctg aacaagttca caatgttccc ccacatactc catgatttca tccttgattg
     1441 gatatcctat caatacctta ggctttacca tcctgattcc tccttacctt ttccttaatt
     1501 ccggcacaat atatggactg tctccctttt ccaggacctc cacaatggcc ttggccgcaa
     1561 ccatcgccat ctcatccctt gtcttccttg ttgctgatcc tatgtgggga agggtgacaa
     1621 cattcctcat ctggagcaag ggattcttcc tgtcaattgg ctccctctca tatacatcaa
     1681 gtcccgctcc tgcaatccaa ccctcctgaa gggccttgat caaagcttcc tcatccacaa
     1741 caggacccct tgatgcatta ataaggaagg catcctcctt catcttacca agctccacct
     1801 ccccgatcag gtgatgggtt tcttctgaaa gtggtgtcat gacaacaaga aaatctgcat
     1861 tttggagaac ctggtccatt tccatgtact cagcctggaa tctctcctct gcttgtggct
     1921 ttctttttct gttatggtaa acaatctcca tgtcaaagcc ctgtgccctc ttggcaacag
     1981 cctctccgat ccttcccatc cctattatcc caagggttga atggtggatg tccttaccaa
     2041 agaacttgtc catttcaccc ttttgccagt ttccggcctt ggtgtctgca tcagcctcaa
     2101 caatccttct tccggctgaa atgataagtc ccataataag gtctgcaact gtattgtcca
     2161 ggacccctgg tgtgtttgtc cccaacaccc ccatatcctt catgtcatca agattatagt
     2221 tgttgtagcc cacagagatg ttgctgacaa cctttagttt gggtgcatgc tttaaaagct
     2281 ccttgtcaat cttcatccca acctccagga tcccatccac atcaccaatg tggtccacga
     2341 ttccctgaaa ggtcttcctc tttgaaaagt cccacattac atagtcacaa tggtctccga
     2401 tgtattcctc tacggatttg ggaatttcat ttgcaataaa tatcttaggt ctcttcatat
     2461 tcttctatca tcctttcttt aggcaaatat ggccattaac cctagggttg tggcaaatat
     2521 cagcagcatc tgcagtgcaa agtatggaag gtactccttg tataggtctc ctgtagtgac
     2581 cttcatttca cctatggtca gtacctggca caggtgaagg ggggaatagt agtaacctac
     2641 aaatgaaacg gctacaagga aaaaggtata aatgtgcagg gcattgcttc ccaggtccat
     2701 cattgaaaga aggggcaggg ttattgccag ggaggatgtt gggtagccgg tgatgtatcc
     2761 aaagaagaag gataccaggg tgaagatgac catcactcca agtccccctt ccactcccat
     2821 aagcatcttg ttgatcatct gaagcatgtc gtccatctgt aggattatgt ccttgagtat
     2881 aaggatggag gtgaccagta tgattgtatt gtagtttatt gaattgtatg ccttgt
//
LOCUS       DASRQF010000326         2055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_771869, whole genome shotgun sequence.
ACCESSION   DASRQF010000326 DASRQF010000000
VERSION     DASRQF010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771869"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..809
                     /locus_tag="VFD79_08610"
     CDS             60..809
                     /locus_tag="VFD79_08610"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HZH94152.1"
                     /translation="MSKYWKEFTVPYYDTDREGYIKPENILAYMAETSNWHSDSLHVG
                     FEELSKKGHAWMLLRWEAEIMKYPRAKDRVKVGTWTSSFDRFYATREFVLTGEDGQVL
                     AKATTKWFFLDIKRRRPKRIPEDLQKSYNFVEDFNFSEFTEMKDLDGSIVESGPHKVR
                     RSDIDNNNHVNNIRYIEWIQEGLGEEVYQTMRVQRLGIVYKKEVLLGDSFMTGHVRDE
                     EMPGCFDHVISVDGEINAQGHTKWAEKGLLE"
     gene            complement(811..1302)
                     /locus_tag="VFD79_08615"
     CDS             complement(811..1302)
                     /locus_tag="VFD79_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH94153.1"
                     /translation="MHPMIQKFVDQKFMTQDQGEYIEAAIMNKESVVVSGHRSAGIRP
                     LMASLMAVAKKEFANVQVKGFEDLEKETDFFLIPGIDNLDFEKLVGEAIGVEGTAFVS
                     IKEPEHPVSLMKVLKANYKAGKAVGKKIHTLECIKVDDVPVLDKITMMVLNEKGKIEK
                     TDF"
     gene            1443..1913
                     /locus_tag="VFD79_08620"
     CDS             1443..1913
                     /locus_tag="VFD79_08620"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HZH94154.1"
                     /translation="MEGASQKLGKKKKIALIAHDNMKKELIDWVRENKEILSGHHLVG
                     TGTTSRLIEERTGLPVRGYMSGPLGGDQQVGARIAEGAIDFMIFFWDPLEAQPHDPDV
                     KALLRIAVLYDIPVANNRATADFILSSPHMDREYERHLIDFENVLKDRVRLFNL"
BASE COUNT          638 a          388 c          486 g          543 t
ORIGIN      
        1 tctaaaaaat tgtaaataat tgcaaatttc gtgtataata gtctttggag ggatgaaaaa
       61 tgagcaaata ttggaaggaa tttactgtac catactacga tacagacagg gaaggctaca
      121 taaagcctga aaatatattg gcatatatgg cagaaacatc taattggcat tctgacagtc
      181 tccatgtggg ctttgaggaa ctgtcaaaaa agggccatgc ctggatgctt cttcgctggg
      241 aggcagagat aatgaaatat ccaagagcaa aggacagggt taaggttggg acctggacat
      301 ccagctttga caggttctat gccacgaggg aatttgttct gacaggggag gatggtcagg
      361 tcttggccaa ggccacaaca aaatggttct ttttggatat aaagagaagg aggccaaaga
      421 ggattcctga agacctgcaa aagtcctaca actttgtgga ggacttcaat ttttctgagt
      481 tcacggaaat gaaggacctt gatgggagta ttgtggaatc aggcccccat aaggtgagaa
      541 gaagtgatat agacaacaac aaccacgtaa acaacatccg ctacatcgaa tggatacagg
      601 aggggctggg agaggaagtt taccagacca tgcgtgtcca aagacttgga attgtctaca
      661 aaaaggaagt ccttctgggg gattccttta tgacaggcca tgtcagggat gaagaaatgc
      721 ctggatgctt tgaccatgtt atttctgttg acggggaaat aaacgcgcag ggccatacaa
      781 aatgggcaga aaaaggactt ctagaataat ctagaagtct gttttttcga tctttccctt
      841 ttcattcagg accatcattg taatcttgtc aaggaccggc acatcgtcaa ccttgatgca
      901 ttcaagtgtg tggattttct ttccaacagc cttgcctgcc ttgtagtttg ccttcaatac
      961 cttcataagt gatactgggt gctctggctc cttaatggaa acaaaagcag ttccttcaac
     1021 accgatagcc tcacccacaa gcttctcaaa gtcaagatta tcaatacctg ggataaggaa
     1081 gaagtcagtc tccttttcaa ggtcctcaaa gcccttaacc tgtacatttg cgaattcctt
     1141 cttggccaca gccatcaggg aagccataag aggtctaatt ccagctgacc tgtgtcctga
     1201 aacaacaact gattccttgt tcatgatggc agcctcgata tattctccct ggtcttgagt
     1261 catgaacttc tggtctacaa acttctgaat cattggatgc atgggatcac tcctttatat
     1321 agtatgttga ttacattcta tcacaaaact gaaaaacagt aaggataaaa attgcatttt
     1381 agttgtcttt catggaaagt tttttcacct gtgatataat tctagtgtga ggaggagatt
     1441 ggatggaagg tgcaagccaa aaattgggga aaaagaaaaa gattgccctc atagcccatg
     1501 acaacatgaa aaaggaattg atcgattggg tgagggagaa caaggagata ctgtcaggtc
     1561 atcatctggt tggaacaggg accacctcaa gactgattga ggagaggacg ggactacccg
     1621 taagaggcta catgtcagga ccacttggag gagaccagca ggtgggagca agaatcgccg
     1681 agggagccat tgattttatg atattctttt gggatccttt ggaagcacag ccacatgatc
     1741 ccgatgtaaa ggcactttta aggattgccg tcctgtatga tattcccgtg gcaaataaca
     1801 gggcaacggc agatttcata ttaagctccc cacatatgga cagggagtac gaaagacacc
     1861 ttattgactt tgaaaatgtg ttaaaggaca gggttagact attcaacctg tagaattgtc
     1921 agaaaagcct atgaattccg gtaattttat gtaaattagc aaaaatccct tgcaatctgg
     1981 ggataaaggt ggtattatgg acatgagctt taattcagga gtaataaaaa gaaacaacaa
     2041 ttttggttta ccttt
//
LOCUS       DASRQF010000327        18043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_70542, whole genome shotgun sequence.
ACCESSION   DASRQF010000327 DASRQF010000000
VERSION     DASRQF010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 18043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18043
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70542"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VFD79_08625"
     CDS             <1..442
                     /locus_tag="VFD79_08625"
                     /inference="COORDINATES: protein motif:HMM:NF036410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5317 domain-containing protein"
                     /protein_id="HZH94155.1"
                     /translation="ALTQASLSLAKRFEIEGLYIFLEDYFLYVMVISYALLLIAIILN
                     IEKNYMKVLFIGVMLNAAVIAANEGKMPVSMTGISGIHQESVIPERESDIKHIGVNHN
                     TKLVYLADIILIPRPYPLPKILSIGDVFLMAGVFLFFQKEMKPR"
     gene            531..1712
                     /locus_tag="VFD79_08630"
     CDS             531..1712
                     /locus_tag="VFD79_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HZH94156.1"
                     /translation="MANKAVILGTNYYIGLSIIRTLGYEGVDTIAVDYTDKDLYGSKS
                     KYLGSHVMAPDFKNHKEEYLKKLIQIGMGEKEKPVLFPSADPYAEFIDENLEELKKYY
                     LIPMEEQGLWTRVMKKETLNDMARENGVLVPETIMPDEENLYSRVETELGYPCIVKPT
                     DSPAFVSVFRRKIFFCKDEEELKTALQRAKEENLEVFVQRIIPGFDDHMHTFDYYVDR
                     SGKVTHWMTCRKLRQFPINFGASVYTEQVHVPELAEIGIPFVEKIGYRGFGEIEFKKD
                     AKTGRFYLIEINTRTTTLNELLYRAGINFPYVAYRDMTGNPLEPKAVEKSTGLVFQYL
                     FEDILAIRDYIRNGQLTTGAVIKSLFRKKVPAVWSFDDPSPGLNYVSIILGKVIKKVF
                     R"
     gene            1722..3278
                     /gene="murE"
                     /locus_tag="VFD79_08635"
     CDS             1722..3278
                     /gene="murE"
                     /locus_tag="VFD79_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl-tripeptide synthetase"
                     /protein_id="HZH94157.1"
                     /translation="MILEDLLKSMDFTDARNLDPNLEIQGVACHSARVKPGFIYVAIK
                     GYVTDGHLYVGKALEGGAAAIVVEDFMDEFDKTCQIKVKDSRKALSRLSSSFYGNPSR
                     KLKVIGVTATNGKTTTTFILDHIYEMAGYRTGLIGSVLAKTGKHTELASLTTPESLDL
                     QRIFSEMAEEKMDRAVMEVSSSALELSRVSDIDYDIVSFNNFSREHIDQHGSYERYWE
                     VKSSLIRDAKPEAVAILNLDNDRISSLVNETKAHLVTISVYGKGGMITCSNLKLVKGR
                     GAFDVVIEDDIPLANGKIQKGSFHVDLKIPGYHSVENAMMAIAISLVDGVDRQTIIDG
                     LKDFGGVERRFEFIYENKITIIDDHFANIKNINSTLGTIADMHRNKVHIAYAIRGNRG
                     VTVNRENAEALVNWKDRLGIDEIIATRSIGSVTQKDKVSQEEEETFKEVLDRSGLGYT
                     IYDTLEEAIGITLERSGEDDIVLLAGCQGMDHGAGIALERIHKDNPHLDREALYKPLK
                     KRVSEGGFLL"
     gene            3359..3871
                     /locus_tag="VFD79_08640"
     CDS             3359..3871
                     /locus_tag="VFD79_08640"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HZH94158.1"
                     /translation="MDLKSKIREIEDFPIEGVSFKDITTLTRDAEAFTSAVSLMVEDL
                     KDKNVDYIVGPEARGFLFGAAVAYGLGVGFVPIRKPGKLPGDTTSYEYELEYGTNTLE
                     IHTESIEKGDRVAIVDDLLATAGTVSAATALIESLGGEVVALEFVIELTFLNGRDKLK
                     DHYINSLVKY"
     gene            3927..5756
                     /gene="glmS"
                     /locus_tag="VFD79_08645"
     CDS             3927..5756
                     /gene="glmS"
                     /locus_tag="VFD79_08645"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HZH94159.1"
                     /translation="MCGIVGYVGRKNAQNVILDGLEKLEYRGYDSAGVALIENGSLLT
                     RKHAGRLSNLVESLDENPIKGYVGIGHTRWATHGAPSDINSHPHTNKEGSIAVVHNGI
                     IENYRALKEELVEKGYTFVSETDTEVVAHLIDFYYKGDLLEAVFSAEERLQGAYALGV
                     VAKNNPQEIIAVRNESPLILGIGSHEHFIASDIPAILKYTNKVIYLENRDVVRLTEEG
                     YEVYTTGRKPVQREIKEIQWDVESASKEGFDHFMLKEIYQQPSAIKDTLERRLDENGD
                     IYLENIAIDEGTLKNIDKIYIVGCGTAYHAGLVGKYLLQTFMEVNVIADIASEFRYGE
                     HFIDEKTLVVIVSQSGETADTLAALKDAKSKGARILSITNVVGSSVARESDDIFYTWA
                     GPEIAVASTKAYTTQLVAFYMLALYFGRLKGTLLEECYREILTELRSLSQKVEDSLET
                     SKENAKAIGGLIKDKKSLFYLGRGLDYFTAMEGALKLKEISYIHTEAFPAGELKHGTI
                     ALIEEGTPVIALATQSRLYDKMVSNIKEVKARGAFVIGVTRRSNADLEEVCDRVIYLE
                     ETFDIFMPLLAVLFPQLLSYHTSLIRGNDVDKPRNLAKSVTVE"
     gene            complement(5899..6261)
                     /locus_tag="VFD79_08650"
     CDS             complement(5899..6261)
                     /locus_tag="VFD79_08650"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZH94160.1"
                     /translation="MSTIGQRLRTARKSKKLSLLDVRDLTGLSTGNLSDLENDKFSPS
                     ANTLIQLRQVLNVSIDWMLTGTLPKDIPSEEIIREKTPAYLSNDEKMLLDMYHRLDSE
                     DRKNIIGYMNLIAGKERG"
     gene            6417..6608
                     /locus_tag="VFD79_08655"
     CDS             6417..6608
                     /locus_tag="VFD79_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZH94161.1"
                     /translation="MRKRNLTPFGYQVKQRLVQINLTQKKLAEKIGTSEVYLSMILYG
                     ERSGKKYIPKIIEALDMGE"
     gene            6762..7844
                     /locus_tag="VFD79_08660"
     CDS             6762..7844
                     /locus_tag="VFD79_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH94162.1"
                     /translation="MKKLLSLLLVLVLSMSLLAGCTQPAEEPAEAPAETPAETPEETP
                     EEPAEEPAEAGAIAKVGLGHVISIAKSTDAEADKTAQAQSDVTVIAVGFDAEGKVVSA
                     ELDVAQTRVAFDENMAVSTDREGEFKTKKELGEDYGMAKASGIGKEWFEQAQAFEAWM
                     VGKTVEEITGMALEDGVPAEEDLVSSVTVGVEAYLEALEQAWAEAIETEGGETVGLGI
                     KISIAKSKDATADAGAMAQVDNVLAATAFDAEGKVTGAVIDTAQVKIQYDLEGKVETD
                     KEAELKTKNELKEEYGMVKASGIEKEWYEQAAALADWMVGQTVEEIAGMSIEDGKTTE
                     EDLVSLVTVSVGDYIYVVEEAFEIAK"
     gene            7925..9004
                     /locus_tag="VFD79_08665"
     CDS             7925..9004
                     /locus_tag="VFD79_08665"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HZH94163.1"
                     /translation="MKKISLILMLTVLINILSGCSEGVAYNRFNDTFFDSFDTVTQVI
                     GYTKTEDEFEGYMTQIHDRMLELHKLYDKYNTYEGINNIKTINDNAGIEPVKVDREII
                     DLLIFSKDMYYSTNQKTNIAFGAVLSIWSEYRDDAEFDPTNAKIPPMDKLLEAKEHTD
                     IEKVIVDQENMTVYLEDPEMSLDVGAVAKGFAVEIVVRELVEAGFDSWIISAGGNIRA
                     VGQPKDPARDTWGIGIQNPDKNVFGDGSNILDTVFMNDISIVSSGDYQRYYVVDGQRI
                     HHIIDPETLMPASHYRAVNVVIEDSGLADFYSTEVFLLPYQEGRDLVESVAGLEAIWV
                     FADGTIEMTEGFREVSRSGGAIVTD"
     gene            9026..9430
                     /gene="ndk"
                     /locus_tag="VFD79_08670"
     CDS             9026..9430
                     /gene="ndk"
                     /locus_tag="VFD79_08670"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HZH94164.1"
                     /translation="MEKTFVMIKPDGVKRKLIGEIIGRIEKKGLSVVKIVMRDPELSI
                     LEDHYRDHRSKGFYKDLMEFMSSGKLVALQVEGDDSIRIMRSLIGETDPTCASPGTIR
                     GDFANSKTENLVHASDSEDAAKRELLLWFGED"
     gene            9432..10136
                     /locus_tag="VFD79_08675"
     CDS             9432..10136
                     /locus_tag="VFD79_08675"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HZH94165.1"
                     /translation="MKKTIGIIGGMGPLATVKLFEKIVLYTRAEADQDHPHTIVDSNT
                     NIPDRTSFILQGGSNPIEELVKSAQRLKEAGADLLTMPCNTAHYFYDDITSSVDLPFI
                     NMIRETVRSIKNDFPESKVGLLSTSGTIASGVYSKELQREGIEYITPSGQHQEAVMEL
                     IYNIKSGKYESDLTGFYGAIDEMESSGATVVILGCTELSVANDMYPLPESVAYVDPLR
                     ILAKEAILQSGCDINT"
     gene            10168..10836
                     /locus_tag="VFD79_08680"
     CDS             10168..10836
                     /locus_tag="VFD79_08680"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HZH94166.1"
                     /translation="MRKWISTLLIIIGAGLIAYQFLPDLLIKRQVEKGAIVAEEITEE
                     QIQENNQREAEYDFSAIEDVSTTNAVAGARQFKDENVIGILTIDDLGINLPILKGVSD
                     ANLMAGAGTMVPDLTMGDGNYSLASHYMKDPSLLFGNLLNAEEGMQVRITDKINVYEY
                     EIYETVLVPETALYMLDHERADERGKPIISLMTCYYTSKNGQRFFALGELVDTYPYRQ
                     KLVD"
     gene            10957..11142
                     /gene="tatA"
                     /locus_tag="VFD79_08685"
     CDS             10957..11142
                     /gene="tatA"
                     /locus_tag="VFD79_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HZH94167.1"
                     /translation="MGFGRLGMPELILILAIALVIFGPSKLPEIGKSLGKALKEFKAG
                     ADKDFNEKEEADEKEDK"
     gene            11123..11851
                     /gene="tatC"
                     /locus_tag="VFD79_08690"
     CDS             11123..11851
                     /gene="tatC"
                     /locus_tag="VFD79_08690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HZH94168.1"
                     /translation="MRRKTSENEGKNLTLTEHLEELRKRLIYSAVFFIAAALLCYTFA
                     ERIIRDMVDLAGDIDFVFISPAELLLANVKLAMIGGLIIAAPFLIIQIWLFVSPGLNA
                     KERKLVAAAISMGGLFFVVGAVFAYFMVIPIVLVFFMGFQMDIISPMISFNSYMSFVL
                     STIVAFGAIFELPILMILLTRFGIVKVSFIKKNRKYMVLGIFVLAAIITPPDIISQLL
                     LGLPMLVLMELGIFLSGIFAKKED"
     gene            complement(11912..12265)
                     /locus_tag="VFD79_08695"
     CDS             complement(11912..12265)
                     /locus_tag="VFD79_08695"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HZH94169.1"
                     /translation="MKKLMVILLTLAMALSLAACGGGEEPAAEGGEPVVLTGVGEGFG
                     GDITVEVTLEGETITNVEVVEHSESAGISDPAIEEIPAAIVAANSTDVDTVSGATYTS
                     EGIMAAVDAALASAE"
     gene            12406..12915
                     /locus_tag="VFD79_08700"
     CDS             12406..12915
                     /locus_tag="VFD79_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002835188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HZH94170.1"
                     /translation="MSKGKDKYSHKYDMKELKVMTIEMINRRGVELEQIAEIVMDLQK
                     EYYEDLTMEICMENIDAVLSKREIIHAIITGIAIDELTEKKLLPEPLQSIVAADEGLY
                     GIDEILPLSIVNVYGTIGLTNFGYLDKEKIGIIKELDTKKGEACHTFLDDIVAAIAAA
                     AASRIAHSR"
     gene            complement(12957..14312)
                     /locus_tag="VFD79_08705"
     CDS             complement(12957..14312)
                     /locus_tag="VFD79_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004838769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HZH94171.1"
                     /translation="MDFIMDFNSAVNSFVWGPPMLFLLVGTGVYLTIGTKFFSILKLG
                     YVLRNTLLKIFSKDQAGEGEITPFQAVATALAATVGTGNIAGVATAIALGGPGAVFWM
                     WVSAVFGMATKFGEVVLSINYREKTQDGRFVGGPMYYIANGLNLKWLATVFAVFGALA
                     AFGIGNMVQSNSVAASLQESFNISPLVTGLVLAVLTALVILGGIKRIGSVTEKLVPGM
                     AAIYILGAVFIIFMNGSHIPEAFGLIFGNAFTGTAAVGGFAGSTLAMAVRFGVARGVF
                     SNEAGLGSAPIAHAASTTDHPVRQGLWGVFEVFMDTIVICTLTALAILVSGLWNTGVT
                     GAALTTQAFDEAILGGGYIVSIGIMLFAFSTILGWSYYGERCAEYLFGEKAIVPYRII
                     WIPFVVIGAIGGLEFIWDLADTMNGLMAIPNLVGILLLSGTVLRLTKEFFASEKEKGN
                     L"
     gene            14725..15327
                     /locus_tag="VFD79_08710"
     CDS             14725..15327
                     /locus_tag="VFD79_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HZH94172.1"
                     /translation="MAKFYYAVKKGLVPGVYQNWEECKNQVHGFPGAVYKKFNTMDEA
                     QAFIGGIEKPEEIIEETDDVDAAIAYVDGSYNLETGTYSYGVVILSDGEKHTFSGRDE
                     NPDLASMRNVAGELKGAMVAMDWAIAKKKKTLHLHYDYTGIENWAKGNWKTNRDGTRD
                     YKKYYDSIKDRLDVRFVKVKAHSGNELNDEADQLAKDAIL"
     gene            complement(15324..15752)
                     /locus_tag="VFD79_08715"
                     /pseudo
     CDS             complement(15324..15752)
                     /locus_tag="VFD79_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
     gene            15880..17076
                     /locus_tag="VFD79_08720"
     CDS             15880..17076
                     /locus_tag="VFD79_08720"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZH94173.1"
                     /translation="MYGVVLEGGGAKGSYQAGAYRGISENGLEISAVAGTSIGALNGA
                     MIVQGEIETCIDLWNDIRYSDIIRDNDQEINRLLHSRPDRHTVKIVADKLRGVILNKG
                     FDITPFKEMIAKHINEEAIRKSPIEFGISTYNLTDRTPMELFKEDIPQGEMKDYLLAS
                     AYMPFFRWEKIGGKNFLDGWFSDNLPYHMLIHKGYENLIIVRTHQMQPGELDDLTGPD
                     PIIISPSEDLGRTFDLDGKRIRRNMKMGYLDASREIRGLVGNRYYILPKREDFYFDYL
                     KNLGDHRIKALGELLNIDSGPSLSLLFGQIIPRLWSMLDIDKNHDYQWFVISMLEQAL
                     EYLGLDRFDIYPFENLMGYVMENGQGLPCKDNTRYDIMINRFGGLVPFNRGDILLRAS
                     RIMFME"
     gene            17093..17710
                     /locus_tag="VFD79_08725"
     CDS             17093..17710
                     /locus_tag="VFD79_08725"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HZH94174.1"
                     /translation="MIIEKLTEKLKNRIPKPLDAVGKFSVLIPLVKVGDQWEVVYELR
                     ARHMKTQPGEVSFPGGKVEKGESFRTTAIRETVEELGVSPEDIEILGELDFLVSYANF
                     TIHCFLGVLHVDDLDSLELNPDEVDHIFTVPVDFLMETEPLAYELSLKTEDTEDFPYE
                     LIPRGREYNFREGKHQVLFYQYDDHIVWGLTARLTKHLTDIIRDL"
     gene            complement(17739..>18043)
                     /locus_tag="VFD79_08730"
     CDS             complement(17739..>18043)
                     /locus_tag="VFD79_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZH94175.1"
                     /translation="LPIAVPGIKTLLFMFPIVGFQIVSTSYFQATGKPKQAMLLSLSR
                     QVLVLIPALLILPNLFGLRGVWMAGPIADFTSSLITAILVFYSIRYLNTQTEKGLI"
BASE COUNT         5624 a         3373 c         4380 g         4666 t
ORIGIN      
        1 agccttgaca caggcgagtc tttcactggc aaagaggttt gagatagagg gattgtacat
       61 atttcttgag gattattttc tgtatgtaat ggtcatttcc tacgcacttt tacttattgc
      121 aatcatactt aacattgaaa agaactacat gaaggtactc ttcataggag ttatgctaaa
      181 tgcagcagta attgctgcaa acgaagggaa gatgcccgta tcaatgactg ggatttcagg
      241 catacaccag gaatcagtca ttccagaaag ggaaagtgac atcaagcaca ttggagtaaa
      301 ccacaataca aaactggttt atctggcgga tatcatatta ataccaaggc catacccact
      361 gccaaaaata ctcagtattg gagatgtttt cttaatggct ggagtgttcc tgttttttca
      421 gaaggaaatg aaacccaggt agcggaccca atcccatcac tatgtcatca gcactggtaa
      481 atgggatttt ttcatgaaaa gagataatag cctttaagga ggaaagttca atggcaaaca
      541 aagcggttat actgggaaca aactattaca taggacttag cataataagg accctaggct
      601 atgaaggcgt tgataccatt gcagttgact acactgacaa agacctgtac ggatcaaagt
      661 caaaatatct tggtagccat gtaatggcac ctgattttaa gaaccacaag gaagagtacc
      721 tgaaaaagct gattcagata ggaatgggag aaaaggaaaa accggttttg tttccaagtg
      781 cagacccata tgctgaattt attgacgaaa atctggagga gcttaagaaa tactacctta
      841 taccaatgga agaacaggga ctgtggacca gggtcatgaa aaaggaaacc cttaatgaca
      901 tggccagaga aaatggagtc cttgttcctg agacaattat gccagatgag gaaaacctct
      961 actccagagt tgagactgag cttgggtatc cctgcattgt aaagccaacg gactcccctg
     1021 cgtttgtatc ggtcttcagg cggaagattt ttttctgcaa ggatgaagag gagttgaaaa
     1081 ctgctctcca aagggctaag gaggaaaacc ttgaggtctt tgtccaaagg atcatcccag
     1141 gctttgatga ccacatgcac acctttgatt actatgtgga caggtcagga aaggtcaccc
     1201 actggatgac ctgcagaaag ctcagacagt ttccgatcaa ctttggcgca tctgtctata
     1261 ctgaacaggt acatgtgcct gagctggcgg aaattgggat accatttgtt gagaaaattg
     1321 gctatagagg ctttggtgaa atagagttca agaaggatgc aaaaaccggc aggttttacc
     1381 tgattgagat taacaccagg accacaacgc taaacgaact cctgtatagg gcaggtataa
     1441 atttccccta tgtggcatac agggacatga caggaaatcc tctggaacca aaggctgtgg
     1501 agaagagtac cggccttgtc ttccagtacc tcttcgagga tatcctggca atcagggact
     1561 acatcagaaa tggtcagctg accacaggag ctgtgatcaa atcactgttc agaaaaaagg
     1621 ttcctgcagt gtggagcttt gatgatccca gtccaggcct taattatgta tcaattatac
     1681 tgggaaaggt aatcaagaag gtttttaggt aggagggcaa tatgatatta gaagatttat
     1741 taaaatcaat ggatttcact gatgcgagaa atctcgatcc aaatttggaa atccaaggtg
     1801 tagcctgtca ttcggcaagg gttaagcctg gattcatata tgttgcaata aaaggctatg
     1861 tcacagacgg tcacctatat gtcgggaagg cattggaagg tggagccgct gcgattgtcg
     1921 ttgaggactt catggatgaa tttgacaaaa cctgtcaaat caaggtcaaa gacagcagga
     1981 aggcattatc aaggcttagc agctccttct atggcaatcc ctcgcgaaaa ttgaaggtta
     2041 ttggtgtcac tgctaccaac ggaaagacta ccactacctt tatccttgac catatttatg
     2101 aaatggcagg ctataggact gggctaatag gaagtgtcct tgccaagact ggtaagcata
     2161 cagagctggc tagtctgaca acacctgaga gtcttgacct tcagaggatt ttctctgaga
     2221 tggctgaaga gaagatggac agggcagtta tggaagtatc ctcgtccgcc ttggagctgt
     2281 ccagggtaag tgacatagat tatgatatag tttccttcaa caacttcagc agggagcaca
     2341 ttgaccagca cggaagctac gaaaggtatt gggaggttaa gtcatccttg attagggatg
     2401 caaagccaga agctgtagct atactaaatc tggataatga caggatcagt agccttgtaa
     2461 atgaaaccaa ggcccatctg gtgacaatct ccgtctatgg aaagggtggc atgatcacat
     2521 gttcaaacct taagctggtt aaaggcagag gggcgtttga tgttgttatt gaggatgata
     2581 tcccacttgc caatgggaaa attcagaaag ggagctttca tgttgacctt aagattcctg
     2641 gataccattc agtggaaaat gcaatgatgg ccatagcaat ttcccttgtg gatggggtgg
     2701 ataggcagac catcatagat ggtttaaagg actttggtgg cgtggaaaga agatttgagt
     2761 tcatctatga aaacaagatc acaataattg atgatcattt tgccaatata aagaacataa
     2821 acagtacact aggtacaatt gcggacatgc acaggaacaa ggtgcacatc gcctatgcaa
     2881 taagaggaaa cagaggggtt acagtcaaca gggaaaatgc ggaggccttg gtcaactgga
     2941 aggacaggct gggaatagat gagataattg ccactagaag cattggaagc gtaacccaga
     3001 aggacaaggt atcccaagag gaggaggaaa ccttcaaaga agtactggac aggtctggtc
     3061 tgggatatac catatacgat accctggagg aggcaattgg gatcaccctt gaaaggtctg
     3121 gagaggacga tatagtttta cttgcaggat gtcagggtat ggaccatggt gctggaattg
     3181 cacttgagag aatacacaag gacaacccac acctggaccg ggaggcgctt tacaaacccc
     3241 tgaaaaagag agtgtcagag ggaggctttc tactgtaaaa aaatgttttt taatggcaac
     3301 ttatatggta aactaatatg tgagtaggaa aaatatgtag taaagaggag gaaaaatttt
     3361 ggatcttaaa tctaaaatta gagaaattga agatttccca atcgaaggtg tgagctttaa
     3421 ggatataact accctcacaa gagatgccga ggccttcaca tcagctgttt cacttatggt
     3481 ggaggacctt aaggataaga atgttgacta tatagtagga ccggaggcaa gaggtttcct
     3541 gttcggagct gctgtagcat atggacttgg tgttggtttt gtgccaataa ggaagcctgg
     3601 caagctacct ggtgatacca ctagctacga atatgaactg gaatatggta caaatacatt
     3661 ggagatacac acggaatcca ttgaaaaggg agatagggtg gcgattgtag atgacctgct
     3721 ggcaacggcg ggaacggttt cggctgcaac tgccctgatc gaatccttgg gtggagaggt
     3781 ggttgccctg gaatttgtta ttgaacttac attcttaaat ggaagagaca agctgaagga
     3841 ccactatata aattcactgg tgaagtacta gacgtcggct tgctgacgtc tttttttcgg
     3901 tgtaggtatt cataaggagg aataatatgt gtggaatagt tggatatgtt ggtagaaaga
     3961 atgcccagaa tgtaatactt gacggactgg aaaagcttga gtacagaggc tatgactcag
     4021 caggggttgc tttgattgaa aacggttccc tgttgaccag aaaacatgct gggcggttga
     4081 gtaaccttgt ggagagcctg gatgagaatc caataaaggg atatgtggga ataggtcata
     4141 ccaggtgggc aacccatgga gccccttctg atataaattc ccatccccat acaaataagg
     4201 aagggtcaat agcggttgtc cacaacggaa taattgagaa ctacagggcc ttgaaggaag
     4261 agctggtgga gaaaggttac acctttgtct ctgaaactga cacagaggtg gtggctcatc
     4321 tgattgattt ctactacaag ggtgatctgc tggaagcggt attttccgct gaggaaagac
     4381 tccagggggc atatgccctg ggagtggttg caaagaacaa cccccaagaa atcatagctg
     4441 tcagaaatga aagcccattg atcctgggaa ttgggtccca tgaacacttt atagcatcag
     4501 acatccctgc gatactaaag tacacaaaca aggttatcta tcttgagaat agggacgttg
     4561 taaggcttac ggaagaaggc tatgaagtgt acactacagg taggaagcct gtccagagag
     4621 agataaagga gatccagtgg gatgtggagt ctgcaagcaa ggaaggtttt gaccatttca
     4681 tgctaaagga aatctaccag cagccttcag caatcaagga cacccttgag agaaggcttg
     4741 atgaaaatgg tgatatttac cttgaaaata tagccataga tgaaggtaca ctaaagaaca
     4801 tagataagat ctatatagtg ggctgtggta ctgcatacca cgcagggcta gttggcaaat
     4861 acctgcttca gacctttatg gaggtaaacg taatagctga tattgcctca gaattcaggt
     4921 atggagagca tttcatagac gagaagacac ttgttgtcat agtcagtcag tcaggggaga
     4981 ctgcagatac cctggcagcc ctgaaggacg caaaatcaaa gggagcgaga atcctttcca
     5041 ttacaaacgt ggtgggaagc tcagtggcca gggaatctga tgacatattc tacacctggg
     5101 ccggtccaga aatagcagtg gcatcaacca aggcctacac aacacagctt gtagccttct
     5161 atatgcttgc cctatacttt ggaaggctca agggtacact gcttgaagaa tgctacaggg
     5221 aaatccttac agagcttagg tctctttcac aaaaggtcga ggattcgttg gagactagca
     5281 aagaaaatgc aaaagccata gggggactta taaaggataa gaaatctctg ttctaccttg
     5341 gaagaggcct tgactacttt actgccatgg agggtgcctt aaagctaaag gagatttcct
     5401 acatccacac agaggcattc ccagctggtg agctgaagca tggaacaatt gcactaatag
     5461 aggaaggaac ccctgttatt gcattggcca cacaatcaag actatacgac aaaatggtgt
     5521 ccaatataaa ggaagttaag gcaaggggag cttttgtcat aggtgttact aggcgcagca
     5581 atgctgacct tgaggaagtg tgcgacaggg tgatatacct tgaggaaaca ttcgacattt
     5641 ttatgcccct gttggcagtg ttgttccctc agttattgtc ctaccataca tcactgatca
     5701 gaggaaacga tgttgataag ccaagaaatc tagcgaaatc agttacagtt gaataaaaga
     5761 agacagttaa aagccgtatt ccttggaata cggctttgtt ttcgcaattg atgttattta
     5821 gagacgattt tgggtaaata aataatatcc atattttgga ataagaagtg gttaataatt
     5881 aatcatggga taaaaaaatt aacctctttc ctttccggca atgagattca tgtagccaat
     5941 tatgtttttt ctgtcttcac tgtccaacct gtggtacata tccagcagca tcttttcatc
     6001 attgctaagg taggctggag ttttttccct aatgatctcc tctgagggaa tatcctttgg
     6061 tagagtgcct gtcagcatcc aatctatgga aacattaagt acctgccgta attgtatcaa
     6121 ggtatttgca gatggggaga acttatcatt ttcaaggtca cttaaattgc cagtggatag
     6181 gccggtaaga tccctgacat ccaacagtga aagttttttt gattttcttg cggttcttag
     6241 tctttgtccg atggtggaca ttgtttcacc tccgtattga tattaatatt gatcgtctta
     6301 tcgctttaac gtcacaatac atatattcta ccacatgtaa gtggataaat gctagaagaa
     6361 agtatttgtg gagagggtag gatagagtag actttcataa gaaggagtga ttttaaatga
     6421 ggaaaagaaa tctcacacca ttcggttacc aggttaagca gcgactagta cagataaatc
     6481 tcacacagaa gaagctggca gaaaaaattg gaacatcaga agtgtacctg agcatgattc
     6541 tgtatggaga aagatccgga aaaaaatata ttccaaaaat tattgaggcc cttgacatgg
     6601 gagaataagc tggaaggttg ggattaggcc atttacgaaa atgacataat cccattatgc
     6661 attgataaat ataaatttaa cgatatgtat tgattacact tcatccctgt ggtataatca
     6721 taatgttgtt tgaacctata aaaaatcatg gagggtgaag aatgaaaaaa ctattatcat
     6781 tattattggt acttgtactt agtatgagtc tacttgcagg atgtacacaa cctgctgaag
     6841 aacctgcaga agcaccagcg gagaccccag cggaaactcc tgaagagaca ccggaagaac
     6901 cagcagaaga gcctgcagag gcaggtgcaa tcgcaaaagt gggtcttgga catgtaattt
     6961 caattgccaa gtcaactgat gcagaggcag acaagacagc acaggcacaa tcagatgtaa
     7021 cagttattgc agttggattt gacgctgaag gtaaagttgt aagcgctgag cttgatgtag
     7081 ctcaaactag agttgcattc gacgagaaca tggcagtttc aactgacaga gaaggcgagt
     7141 tcaaaacaaa gaaagagctt ggagaagatt atggaatggc taaagcctca ggcattggca
     7201 aagagtggtt cgaacaagca caagcttttg aagcatggat ggttggaaag actgtagaag
     7261 aaattacagg aatggctttg gaagatggag ttccagctga agaagaccta gtgtcatcag
     7321 tgactgtagg agtggaagct taccttgagg cacttgagca ggcatgggca gaggctattg
     7381 agactgaagg cggagagact gtaggccttg gaatcaagat ttcaattgcc aagtcaaagg
     7441 atgctacagc agatgcagga gctatggctc aggtagacaa tgtgttggca gcaacagcat
     7501 ttgacgctga aggaaaagta actggtgctg taattgacac agctcaggtt aagattcagt
     7561 acgaccttga aggaaaggta gaaactgata aggaagcaga gcttaagacc aagaacgagc
     7621 ttaaggaaga atacggtatg gttaaggctt caggcatcga aaaagagtgg tatgagcagg
     7681 cagctgcatt ggcagactgg atggttggcc aaactgtaga agaaattgca ggcatgtcaa
     7741 ttgaagatgg aaaaaccact gaagaagacc tggtttcatt agtaactgtc agtgttggag
     7801 attacatcta cgttgttgaa gaggcttttg aaatagcaaa gtaaattaat tatggctccc
     7861 ttttgggagc cattttgttt gcctgtaaag gctatttttg gtatgattag ttagggtgat
     7921 tttgatgaaa aaaattagtt tgatattaat gttaactgtc ctgataaata ttctgtcagg
     7981 atgttcagaa ggagttgcat acaatagatt caacgatacc ttctttgatt cattcgatac
     8041 ggttacacag gtaattggat acacaaagac tgaggatgag tttgagggat acatgaccca
     8101 aattcacgac aggatgctgg aactgcacaa actgtatgac aagtacaata cctacgaggg
     8161 tatcaacaac atcaagacca ttaatgataa tgctggtatt gaacctgtaa aggtggatag
     8221 ggaaataatt gatcttttga tattctccaa ggacatgtac tacagcacaa accagaagac
     8281 caacatagct tttggagcgg ttctttcaat ctggtcagag tatagggatg atgccgaatt
     8341 tgatccgacc aatgcaaaga ttcccccaat ggacaagctc ttggaggcaa aagaacacac
     8401 ggacattgag aaggtcatag tggaccagga aaacatgact gtctatcttg aagaccctga
     8461 gatgagcctt gatgtcggag cggtagccaa ggggttcgcc gttgaaatag ttgtaagaga
     8521 gctggtggag gcaggctttg actcatggat tatcagtgct ggagggaata tcagggctgt
     8581 gggccaacca aaggatcctg ccagggatac atggggcatt ggaattcaga acccagataa
     8641 gaatgtattt ggagatggtt caaacatact tgatacagtg ttcatgaacg acatctctat
     8701 agttagctcg ggagattacc aaaggtacta tgtggttgac ggacaaagga ttcaccacat
     8761 aatagacccg gagacactta tgccagcttc acactacagg gcagtgaatg ttgttattga
     8821 ggattccgga cttgctgact tctactcaac agaagtattt ctacttccat accaggaggg
     8881 cagggacctt gttgagtccg ttgcgggact ggaagcaata tgggtatttg cagatggaac
     8941 aattgaaatg acagaagggt ttagggaggt ttccagatcc ggtggagcca tagtcacaga
     9001 ttaaaattag tcagggggag ttatcttgga gaagactttt gttatgatca agccagatgg
     9061 agtcaagcga aagctcatag gggagattat cggtaggata gagaaaaaag gcctgtcagt
     9121 ggtaaaaata gtaatgagag acccggagct ttctattttg gaagaccact acagggacca
     9181 caggtcaaaa ggtttttaca aggacctgat ggagtttatg tccagtggaa agcttgtggc
     9241 ccttcaagtg gagggggatg attcaataag aatcatgagg agccttattg gggaaacaga
     9301 ccctacatgc gcatcccctg ggacaataag aggtgacttt gcaaactcca aaactgagaa
     9361 tctggttcat gcttccgact cagaggatgc tgcaaaaaga gagttgctac tttggttcgg
     9421 ggaggattag aatgaagaag acaattggga taatcggagg aatgggtcct ttggcaacgg
     9481 tgaagctatt tgaaaaaatt gttctttata ccagggcaga agcggaccag gaccatccac
     9541 acacaatagt tgacagcaac acaaatatcc ctgacaggac aagctttatc cttcagggag
     9601 gatcaaatcc aatagaggaa ctcgtcaaat ctgcacaaag gcttaaggag gcaggggcag
     9661 atttgttgac tatgccatgt aacacggcgc attacttcta tgatgatata acaagttcag
     9721 tggacctgcc ctttataaac atgatcagag agactgttag gtcaatcaag aatgattttc
     9781 ctgaatcaaa ggtggggctt ttgtccacca gtggaacaat tgcttcgggt gtgtacagca
     9841 aggaactgca aagggaaggt atcgaatata taactccctc aggacaacat caggaggcag
     9901 ttatggagtt gatttacaat atcaagagcg gcaagtatga aagtgacctg acaggattct
     9961 atggagccat agatgagatg gagtcaagtg gagccacggt cgtgattctt gggtgcacag
    10021 agctttcggt agccaatgac atgtatccct tgcctgaatc tgtagcctat gttgatcccc
    10081 taaggattct tgcaaaagag gccattttgc aatcaggatg tgatattaat acctaacgtg
    10141 ggtatctatt gatagaggtg aaatgctatg agaaaatgga tatcgacttt attgataata
    10201 ataggagcag gcctgattgc ctatcagttc ttaccagatt tgttgattaa gcgtcaggtt
    10261 gaaaaaggtg caatagtggc tgaggaaatc actgaagagc agattcagga gaacaaccag
    10321 cgggaagcgg agtatgattt ttctgccatt gaggatgtgt caaccaccaa tgcagtggct
    10381 ggagcaagac agtttaagga cgaaaatgtt attggcatct taaccataga cgatctggga
    10441 attaaccttc ccatactaaa gggtgtttcc gatgccaacc tgatggcagg agccggaaca
    10501 atggtacctg accttaccat gggtgatggg aattattcac ttgcatcaca ttatatgaag
    10561 gatccaagtc tgctatttgg gaacctttta aacgcagagg aaggaatgca ggtcaggatt
    10621 acagacaaga taaatgtata tgaatatgaa atatatgaga cagtgctggt tcctgaaact
    10681 gcgctctaca tgcttgacca tgaacgtgca gatgaaaggg gaaagccaat aatatcactg
    10741 atgacctgct actacacctc gaagaatggc cagaggttct ttgcccttgg ggagcttgtg
    10801 gacacatacc cctacaggca gaagctagtg gattaaaagt cctggtttgg attgatatat
    10861 agacaataat caaattgaag ttgtattgca gactgattag tggtagaatc attctatgag
    10921 gtttgacaaa attatatcga tataggaggt tgaattatgg gatttggaag actgggaatg
    10981 cctgaactaa tattgattct agcaatcgca ttggttatat ttggaccgtc aaagctgcct
    11041 gaaataggga aatcattagg taaagcacta aaggagttca aggctggagc ggacaaggat
    11101 tttaatgaga aggaagaagc tgatgagaag gaagacaagt gaaaacgaag gtaagaacct
    11161 tacattgact gagcacctag aggagcttcg caagagactg atatactctg cggtgttctt
    11221 catagctgca gcactcctat gctacacatt cgctgaaaga attatcaggg atatggtaga
    11281 tctggctgga gatattgatt ttgttttcat atcccctgca gagctcctgc tggccaatgt
    11341 gaagctggcc atgatagggg gacttataat agcagctcca tttctaataa ttcagatttg
    11401 gttgtttgtc agtccgggat tgaacgcaaa ggagagaaaa ctcgttgcag cagcaatatc
    11461 aatgggaggg ctgttcttcg tagtgggagc ggtttttgca tattttatgg taataccaat
    11521 agtattggtg tttttcatgg gtttccagat ggacataata tcacccatga tcagcttcaa
    11581 cagctacatg agctttgtgc tcagtacaat agttgccttt ggggcaatat tcgaattgcc
    11641 tatactgatg atactcttga ccaggtttgg aatcgtcaag gtaagcttta ttaagaagaa
    11701 cagaaaatac atggttctag gtatatttgt cctggctgca ataattacgc cgccagatat
    11761 tatatcacag ctgctgctgg gtctacccat gctggtactt atggaactgg gaatattcct
    11821 atctggaata tttgcaaaaa aagaagacta ggaattaaga aacccttatc ggcttgggcg
    11881 ataagggttt tctcttcatc tatctattag attactcagc gcttgctaga gcggcatcta
    11941 ctgctgccat gataccttca ctggtgtatg tagcacctga aaccgtatca acatcagttg
    12001 agtttgctgc aactattgct gcaggtatct cttcgattgc tggatcactt attcctgctg
    12061 attcactatg ctcaactact tcaacatttg ttatagtttc gccttcaagt gtaacttcaa
    12121 cagtaatgtc tccgccaaag ccttctccta caccagtaag aactacaggc tcaccacctt
    12181 cagctgctgg ttcttctcct cctccacatg ctgcaaggct caaagccata gctagtgtca
    12241 aaaggataac cattaacttc ttcattgtat tcaactcctt tcatttaaat gtactaatag
    12301 attatacact tttgttatta aaaaatcaag ttccccgaaa gatgaagtgg gctcaggtga
    12361 caatgttggg tatacataaa aggaaaactt aaaggagggc gaataatgtc aaaaggaaaa
    12421 gacaaataca gccacaagta cgacatgaag gaacttaagg tgatgacaat tgaaatgatc
    12481 aacaggagag gagtggaact tgaacagatt gcagagattg taatggacct tcaaaaggag
    12541 tattatgagg acctgacaat ggaaatatgc atggagaata tcgatgctgt acttagcaaa
    12601 agggaaataa tccatgcaat cataactgga atagcaattg atgagcttac ggagaagaag
    12661 cttctacctg agcctctaca aagcatagtt gcagctgatg agggcctgta cggaattgat
    12721 gagattcttc cactgtccat tgtcaacgta tacggaacaa tcggactaac caactttgga
    12781 tatctggaca aggaaaagat aggaatcatc aaggagctgg acacaaagaa aggggaagca
    12841 tgccacacct tcctggatga cattgttgcg gcaattgcag cagcagcagc cagcaggata
    12901 gcccattcaa gataatatag cataaaagaa gcgaggccta ggcctcgctt caataattac
    12961 aagtttcctt tttctttctc gcttgcaaag aattcctttg taagccttag aactgttcca
    13021 ctaagcagaa gtataccaac aaggtttgga attgccatta gaccgttcat ggtgtctgca
    13081 agatcccaga taaactccaa tcctccgatt gctccaatta ctacaaatgg aatccagatt
    13141 attctgtaag gtacaattgc cttttcacca aacaggtact cggcacatct ctctccatag
    13201 taggaccagc ccagtattgt tgagaatgca aacagcatga tacctattga tacgatgtat
    13261 cctccaccta ggatagcctc atcaaatgcc tgtgttgtaa gtgctgcacc tgtgactcca
    13321 gtgttccaaa gacctgatac aagtattgca agagctgtta gggtacagat tacaattgta
    13381 tccatgaata cctcaaatac tccccaaagt ccctgtctaa caggatggtc agtggttgaa
    13441 gctgcgtggg cgattggtgc actaccaagc ccagcctcgt ttgagaatac tccacgagca
    13501 actccgaatc tcactgccat tgcaagtgtt gaacctgcaa atccacctac ggcagctgtt
    13561 cctgtgaatg catttccaaa gattaggcca aatgcctcgg gaatgtgtga tccgttcatg
    13621 aagattatga atactgcgcc aagaatatat atcgctgcca ttcctggaac aagcttctcg
    13681 gttacggaac caattctctt gataccacca agtattacaa gagcagtcaa tactgcaaga
    13741 acaagacctg ttacaagtgg ggaaatgtta aatgactcct gaagtgaggc cgcgactgag
    13801 tttgactgta ccatattacc gatgccgaag gctgcaagag ctccaaatac cgcaaataca
    13861 gtagccagcc acttaaggtt aaggccgttg gctatgtagt acataggtcc accaacaaaa
    13921 cgtccatctt gagttttctc cctgtaattg attgaaagaa caacctcgcc gaacttagtt
    13981 gccataccga acactgctga tacccacatc cagaatactg ctcctggtcc accaagggct
    14041 atggctgttg caactccggc aatgttacct gttcccacag ttgctgccaa tgcagttgca
    14101 actgcctgga atggcgtgat ttcaccttct cccgcctgat cctttgagaa gattttcaat
    14161 aaagtgtttc taagtacata acctagttta agaatagaaa agaatttagt tccgattgtt
    14221 aagtaaactc ctgtacctac caataggaat agcataggtg gtccccatac aaaactattg
    14281 acggcactgt taaaatccat aataaagtcc ataaatacct cctagtagtt tttgtcaatt
    14341 tcatctgttt caacaattgg tgggttgttg agggttgtag gttaaacgtt ttccccgtac
    14401 gtttaaaaac aaccgatttc tattttaaca gaaactcttt tggaattcaa tacaatttat
    14461 agaaaatttg aaatatacag taaatagata caaatataac aaagaattac gaacaaatgt
    14521 tgataaaaca caaaaatata acaactacaa tcaatatatg ggttaacata tgtggttcag
    14581 accaaaatta ttacccggta aacagaactt gcataaataa agtgacaaac atatcacaaa
    14641 cagctggaag ttacttccct ttctacaatt tgatttggaa tatgtgttat aatctataca
    14701 gaataaggat ataagaggtg gatcatggca aaattttatt acgcggtaaa aaaaggtttg
    14761 gttccaggtg tttatcaaaa ctgggaggag tgtaaaaacc aggtacatgg atttccgggg
    14821 gctgtttaca agaaattcaa tacaatggac gaggcccagg cctttattgg aggtattgaa
    14881 aagcctgagg aaataattga agaaaccgat gatgtggatg cggctatagc ctatgtggat
    14941 ggaagctaca acctggagac gggcacctat agctatggtg tagtaatact atctgatggg
    15001 gagaagcata ctttcagcgg cagagatgag aatccagacc tggcctcgat gaggaacgta
    15061 gcaggtgagc ttaagggagc catggtggcc atggactggg caattgcaaa aaagaagaaa
    15121 accctccacc ttcattacga ttacactgga atcgagaatt gggcgaaggg caattggaaa
    15181 acaaataggg atggtaccag ggactacaaa aaatattatg actccatcaa ggataggttg
    15241 gatgtaaggt ttgtaaaggt taaggctcat tctggcaacg agctcaacga tgaggccgat
    15301 cagctggcaa aggatgcgat cctctagaca atcttttcat aggccacact gtttctctcc
    15361 aatagctcca cggcatagtc atcaaccctg taatcgttct tgtagtatat ttttttaatt
    15421 cctgcctgta taagggattt tgtgcaattc aggcagggga aatgtgtgac ataggcaatg
    15481 caattattca cagggatccc ttcctttgca cagtaaagaa gtgcattcat ctctgcatgt
    15541 atggttgcaa tgcaatgtcc gtcccgcatg gtgtgaccaa catcgtcgca atgtggattt
    15601 cctgacactg aaccgttgta tcctgttgat acgatcctat tgctgtcatt tacaattaca
    15661 caacctattc aggctctgtc gcaggtggac cggcttgcaa ccagctcggt gatttccatg
    15721 aaatacttgt tccaactcat tcttctggac atatgattcc tccttctgta acttaatagg
    15781 aatattatac cacaaggcaa tgagtaggag catcctaata tatgggtata tagtaaagag
    15841 taaaatctta ttcatagacc tttcaggagg tggaagtaca tgtatggagt tgtgcttgag
    15901 ggtggtggag caaaaggctc ttatcaggcg ggagcctaca ggggcatcag tgagaatggg
    15961 cttgaaatat cggctgtggc agggacatcc atcggagcct tgaatggagc catgattgtc
    16021 cagggtgaaa ttgaaacctg cattgacctg tggaatgata tcagatattc cgacataatt
    16081 cgtgacaacg accaggagat caacaggctt ttgcattcta ggcctgacag gcataccgtg
    16141 aagattgtgg ctgacaaact gagaggtgta atacttaata agggctttga tattacaccc
    16201 ttcaaggaaa tgatagcaaa gcacattaat gaggaggcca taagaaaatc tcccattgag
    16261 tttgggatat ccacctataa ccttactgac agaaccccaa tggagctttt taaggaggac
    16321 ataccccagg gtgagatgaa ggactatctt ttggcttcgg cctacatgcc atttttcagg
    16381 tgggaaaaaa ttggcggcaa gaactttctg gatgggtggt tctctgacaa tctaccctac
    16441 cacatgctaa tccataaagg ttatgaaaac ctaataatag taaggaccca tcagatgcag
    16501 ccgggagagt tggatgattt gacaggacct gatcctatta taatatcacc aagtgaagac
    16561 cttggtagaa catttgacct tgacggcaag agaatcagaa gaaacatgaa gatgggatac
    16621 cttgatgcgt ccagggaaat cagaggtctg gttgggaata gatactatat cctgccaaag
    16681 agggaagatt tctactttga ttatcttaaa aatctgggag accataggat aaaagccttg
    16741 ggtgaacttt tgaacatcga ttcaggaccc agcttaagtc tcttgtttgg gcagataatt
    16801 cccaggctgt ggtccatgct ggatattgac aagaaccatg attatcaatg gtttgtgata
    16861 tcaatgcttg aacaggccct ggaatacctt gggctggaca ggtttgatat ttacccgttt
    16921 gaaaatttaa tgggctacgt catggaaaat ggacaagggc taccctgtaa ggacaatacc
    16981 agatatgata taatgatcaa caggtttggt ggacttgtcc catttaacag gggcgatata
    17041 cttttaagag cttctagaat aatgtttatg gaatgatttg gaggatgctg agttgattat
    17101 agaaaaactg acagaaaaac taaagaacag aatacctaag ccattggatg cagtagggaa
    17161 gttttcagta ttgatccccc tggttaaggt aggtgatcag tgggaagtgg tttatgagct
    17221 tagggcaagg cacatgaaga cacagccagg agaggtttca ttccccggtg ggaaggttga
    17281 gaagggagaa agcttcagga ctacggcaat aagagaaaca gtggaggaat tgggcgttag
    17341 ccctgaggac attgaaatac ttggagaatt ggactttctt gtatcctatg ccaactttac
    17401 catacactgc tttcttggtg tgcttcatgt tgatgacctg gatagtctgg agcttaaccc
    17461 ggacgaggtg gaccacatat tcacagtacc tgttgacttt cttatggaaa cagaacccct
    17521 tgcatatgag ttgtctctta agacagaaga tacagaggat tttccctatg agcttatacc
    17581 cagaggcaga gagtataact tccgtgaggg aaaacaccag gtccttttct accagtacga
    17641 cgaccacata gtatggggtc tgaccgcccg gttgaccaag cacctgacag acataatccg
    17701 tgacctgtaa aattaagagg actgaatcca gcccaaggct agatcagtcc tttttccgtc
    17761 tgggtattta gatacctaat ggagtagaat acaagaattg cagtaataag tgatgaagtg
    17821 aaatcggcaa tgggaccagc catccatact cctcttaacc cgaacagatt aggcagtata
    17881 agcagggccg gtatcagtac aagaacctgt cttgaaagac tcaatagcat ggcctgcttc
    17941 ggcttccctg tagcctggaa gtaggatgtg cttacaatct ggaagcctac aatggggaac
    18001 atgaacagga gtgttttaat accggggact gcaattggca gca
//
LOCUS       DASRQF010000328         9320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1680584, whole genome shotgun sequence.
ACCESSION   DASRQF010000328 DASRQF010000000
VERSION     DASRQF010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 9320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9320
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680584"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1012)
                     /locus_tag="VFD79_08735"
     CDS             complement(44..1012)
                     /locus_tag="VFD79_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HZH94176.1"
                     /translation="MTLNKEEGSIEINDDKTIKEIAGNLDANIRTIEEKMKVRLRIKD
                     NAITVQGLPENVEMTKRLLDDLKYMIQKQNTLQEQDLNYAIDFIRSRKEGSLKELLDD
                     VVCITASGKSIKPKTFGQKRYVDSMRKHDVVFGIGPAGTGKTYLAMAMAVQAFKNREV
                     NRIILTRPAVEAGENLGFLPGDLQEKVDPYLRPIYDALFEILGVDTYLKFFEKGLIEV
                     APLAYMRGRTLDSAYVILDEAQNTTNEQMKMFLTRLGFGSKAIITGDITQIDLIRGKE
                     SGLKTVRGILEDVKGIDFINLTSSDIVRHPLVQRMINAYEIHEGRK"
     gene            complement(1148..1705)
                     /locus_tag="VFD79_08740"
     CDS             complement(1148..1705)
                     /locus_tag="VFD79_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH94177.1"
                     /translation="MDSPIIILVFWVIINILLKSSRDKKKAEQARRKSGQANAPDRQR
                     QKPERSRMNTGMKDFRQALEDYKAQIEKELNPEKKSPAKPPAKVPTTEARQRVERSDS
                     RLEKPKMKSFREGRYWEDITDGMADSDTDKETAGIVVAIEETEKKAKGSSEGLFDIKN
                     DILKGIVYSEILGKPKSMQGKRGPF"
     gene            complement(1717..2697)
                     /gene="floA"
                     /locus_tag="VFD79_08745"
     CDS             complement(1717..2697)
                     /gene="floA"
                     /locus_tag="VFD79_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244797.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HZH94178.1"
                     /translation="MDLIFTLAIAIGIIVLLMLFFTFVPVGLWITAFFAGVKVRITTL
                     IGMRLRRVVPSRIINPMIKATKAGLDINIDDLESHYLAGGNVNTLVDALIAAQRANIP
                     LVFERAAAIDLAGRNVLEAVQVSVNPKVIETPQIAAVAKNGIEVVARARVTVRANIER
                     LVGGAGEETIIARVGEGIVTTVGSASSHKDVLENPDSISKTVLEKGLDAGTAFEILSI
                     DIGDVDVGRNIGAKLQMEQAEADKKIAQAKAEERRAMAVAREQEMRAEVQAMNAKLVE
                     AEADVPKALAQALREGKLGVMDYYNMKNILADTDMRDSISKIADDDKETK"
     gene            complement(2699..3991)
                     /locus_tag="VFD79_08750"
     CDS             complement(2699..3991)
                     /locus_tag="VFD79_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HZH94179.1"
                     /translation="MKKIRFLAFFIIVFVIFISSGSAQAQSPGKVYVVPIQGEINRAT
                     HNYVRDVVNDLNNEPDVEAIIFEIDTYGGVVDEAIKIKDVIIGTNIPTITLVNNKAAS
                     AGVLVTIAGEHIAMSENAVIGSAETIPNTEKILSMWRGVLRDTAQYRGRDALLVESMA
                     DSDIAIEGVIDGGKLLNLTAQEAYQLGLADVLSSDYNVILEHFGFEASQVEVMEEGLQ
                     VKLSKYISNPYISTLLLTLGFVGLVLEVLTPGFGLGGTISLMGFGLYFGGNILAGNSN
                     WTSLILFVVGLGLLVIEGVVPGFGLPGIGGLIFVIAGTVLAMQDLATAVLSLSIAIIV
                     TTLVAVVMVKHGYKSKFLNKIVLSNKLESTRGYLSTNTMSDLLDKEGTVLSELRPSGF
                     IIIDGEKYDALAETGYIPENSNVKVVKVEGSKIFVRRI"
     gene            complement(4075..4518)
                     /locus_tag="VFD79_08755"
     CDS             complement(4075..4518)
                     /locus_tag="VFD79_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HZH94180.1"
                     /translation="MSLKDKLMEDLKVSMKNKDTIRKNTVTMVRAAVKQREVDERTEL
                     TDEDILEIVAKQLKEKKMAIEEFKKGSRQDLVDTTESEIKILLEYLPEQMNEEEVERI
                     VKETIEEIGATSIKDIGLIMKATMPKVKGRADGNMVNTAVRKLLK"
     gene            complement(4534..4716)
                     /gene="rpsU"
                     /locus_tag="VFD79_08760"
     CDS             complement(4534..4716)
                     /gene="rpsU"
                     /locus_tag="VFD79_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005957648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HZH94181.1"
                     /translation="MTEIKVGENESLDNALKRFKRSCARSGVIGEVRKREHYEKPSVK
                     RKKKSEAARRKKHSKY"
     gene            complement(4824..5162)
                     /locus_tag="VFD79_08765"
     CDS             complement(4824..5162)
                     /locus_tag="VFD79_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HZH94182.1"
                     /translation="MDCLFCKIAKKEIPSDLVHEDEFVVVFKDINPQAPVHFLVVPKV
                     HIKSLDYITEEHDTLISHVFRLIPKLAKDQGIENGYRVVNNCGPDGGQSVDHIHFHVL
                     GKREMLWPPG"
     gene            complement(5196..6497)
                     /gene="mtaB"
                     /locus_tag="VFD79_08770"
     CDS             complement(5196..6497)
                     /gene="mtaB"
                     /locus_tag="VFD79_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HZH94183.1"
                     /translation="MNSVAFHTLGCKVNQYETEAMEEMFEKKGYTIVEEDGYSDIYVI
                     NTCTVTNLSDRKSRQFIRKSKKNNPGAIVAVVGCYSQVSPEEVEKIQGVDVIIGTSER
                     HRIVDLCEEASNHKRRINIVRSVKTYKEFEKINIDELKSRTRAFVKIQDGCNQFCSYC
                     IIPYARGPIRSREFADIENEVENLAKAGFKEVVLTGIHVASYGKDLKKESLGQVIDKI
                     SLIEGIERIRLSSLEPTLIDDDFMEIMTKSPKICDHFHLSLQSGSDSVLERMNRKYTT
                     QEYRDRVDLIRKYLPDAGITTDIIVGFPGETEDEFNETLRFVEEIGFSRIHVFKYSPR
                     KGTPAATMEDQVHGEIKNKRSEILIALGDRLSEEFNNRYLEGAKEVLYEEHTKRDENV
                     YEGYTTNYIRVRTPSPVDLIGEIRDTRLEAVKGSLIHGTLI"
     gene            complement(6497..7243)
                     /locus_tag="VFD79_08775"
     CDS             complement(6497..7243)
                     /locus_tag="VFD79_08775"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HZH94184.1"
                     /translation="MMHRFFTDNEIDGAGRIRIQGSDVKHARDVLRMGSGEKLEIVVE
                     GKAYLCSIIEIGKNIMVAELVQPLERGHESSIKINLFQGLPKSTKMETILQKATELGV
                     SSFYPLLTNRTVVRLKGEKKQDRKLERWESVVHEAAKQSKRDTIPKVHQAIDMSEIEA
                     YLKEGITIVPYEASEDNGIKDVLRSLEVPRTVNIVIGPEGGFEEDEIDMLVRLGARIV
                     SLGPRILRTETAGIVACAIVQYEFGDLGVI"
     gene            complement(7240..8160)
                     /gene="prmA"
                     /locus_tag="VFD79_08780"
     CDS             complement(7240..8160)
                     /gene="prmA"
                     /locus_tag="VFD79_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HZH94185.1"
                     /translation="MNWLEVIIKTKPENEDLVSSILYDNGATGLTIEDPNDIVELDKA
                     KDAWDFIEPELLKKKDDGIIIKAYYSEAEDLEGILEKIRERIKNQGLGEILTSKVDES
                     DWAENWKIHYKTTKIGANIVIKPSWEEYQPERDDIVIELDPGMAFGTGSHETTVMCAK
                     ALERNVESTSTVFDVGCGSGILGIIAAKLGAGRVLAIDLDPMCIKVTKENAIINQVDD
                     TIQVEQGNLLHMVEEKADVIVANIIAEIIVDLVPGLRNHLNDNGIFIASGIIESKLDK
                     VKEALMSSGYEIVDVELMNGWAAVTSRMVI"
     gene            complement(8287..8421)
                     /locus_tag="VFD79_08785"
     CDS             complement(8287..8421)
                     /locus_tag="VFD79_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH94186.1"
                     /translation="MIIPKDLSDKQRELLLEYSKEMGENYKTPEKKGFFKKVKDAFQQ
                     "
     gene            complement(8470..9267)
                     /locus_tag="VFD79_08790"
                     /pseudo
     CDS             complement(8470..9267)
                     /locus_tag="VFD79_08790"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
BASE COUNT         2236 a         2444 c         1598 g         3042 t
ORIGIN      
        1 agtttttatc ataatcccca cctgccttgg ttcaactgct caactacttc ctgccctcat
       61 gaatttcata tgcgttgatc attctttgaa ccagcggatg tctaactatg tctgaacttg
      121 tgagatttat aaagtctatc cccttaacat cctccaggat tccccttact gtctttagac
      181 cagattcctt acctctaatc aggtcaattt gtgtgatgtc tccggttatt atagcttttg
      241 atccaaatcc aagtctggtc aaaaacattt tcatttgctc atttgttgta ttttgggcct
      301 catcaagaat cacatatgca gaatcaaggg ttctacccct catataggct aggggtgcca
      361 cttctatcag acccttttca aagaacttca gataggtgtc cacacccagt atttcaaaaa
      421 gtgcgtcata gattggtctt aggtatggat ctaccttctc ctgcaggtct cctgggagaa
      481 atccaagatt ctcaccggcc tcaaccgcag gtcttgtaag tattatccta ttgacttccc
      541 tgttcttgaa tgcctgaaca gccattgcca tcgccagata ggttttcccc gtgcctgcag
      601 ggcctattcc aaacaccaca tcgtgctttc tcatggaatc aacatatctt ttctgcccaa
      661 aggttttggg tttgatggac tttcctgaag cggttataca aaccacatca tcaagaagtt
      721 ccttaaggct tccctcttta cgggacctga tgaagtcaat ggcgtaattc aggtcctgtt
      781 cttgaagagt gttctgcttt tgtatcatgt atttcaaatc atcaaggagc ctctttgtca
      841 tttccacatt ctccggcaat ccctgaactg taatggcatt gtcttttatc ctaagcctga
      901 ccttcatttt ttcttcaatg gtccttatat ttgcatctag gttcccagct atctccttta
      961 ttgttttgtc gtcatttatt tcaatggatc cttcttcctt gtttaatgtc aaacttaaat
     1021 tcctccccag tatctttagt ttacctccag tttaattaaa taaatcatac catagaaagg
     1081 tgtggttttc tatatgttta gcttatgtgt aaccatttag taataagttg acaaaaaaga
     1141 agaagtccta aaaaggacct ctttttcctt gcatgctttt tggcttgcca aggatctcag
     1201 aataaacaat acccttgagt atgtcgttct tgatgtcaaa tagaccttcc gagcttcctt
     1261 tagccttttt ttcagtctcc tcaatggcta caacaattcc cgctgtttcc ttatccgtgt
     1321 ctgagtcggc catgccatca gttatgtctt cccagtatcg cccttccctg aaggacttca
     1381 ttttcggctt ttctagtcga ctatcagacc gctcaaccct ttgcctggct tctgtggttg
     1441 gaaccttggc gggaggcttt gccggagatt tcttttccgg gttcagttcc ttttctatct
     1501 gtgccttgta gtcttccaga gcctgcctga agtccttcat cccggtattc atacgggatc
     1561 tctcaggctt ttgtctctgt ctgtcaggtg catttgcctg cccggatttc cttcttgcct
     1621 gttcagcctt cttcttgtct ctggagcttt ttagaagtat gttgattatt acccaaaaga
     1681 ccagaatgat tataggacta tccatttaat cacttcctat tttgtttctt tgtcatcgtc
     1741 agctatcttt gatattgaat ctctcatatc tgtatcagca agtatatttt tcatattgta
     1801 ataatccata actccaagct ttccttctct caatgcctga gccagtgcct ttggaacatc
     1861 tgcttccgct tccacaagct tggcgttcat ggcttgaacc tcagccctca tttcctgttc
     1921 tcttgcaaca gccatggctc ttctttcctc cgcctttgcc tgtgcgatct tcttgtctgc
     1981 ctcagcctgc tccatctgaa gttttgcgcc aatgtttctc cctacatcca cgtcacctat
     2041 atctatggaa agtatctcaa atgcagttcc tgcatccaag cccttttcca gtactgtctt
     2101 ggatatgctg tcaggattct ccaatacatc cttgtgggaa ctggcactac caacagtggt
     2161 aactatacct tctccaactc ttgcgattat ggtttcctcg cctgctccac caacaagtct
     2221 ttcaatattg gccctaacgg ttactcttgc ccttgccaca acttcaatac cattcttggc
     2281 aacagctgca atctgaggtg tttcaataac ctttggattt acacttacct gtaccgcttc
     2341 cagtacattt ctaccagcaa gatctatcgc tgccgctctc tcgaaaacca agggtatgtt
     2401 agccctttga gcagcaatca aagcatccac aagggtgttt acatttcccc ctgcaagata
     2461 atgtgactcc aggtcatcga tgttgatgtc caatccagcc tttgtggcct ttatcattgg
     2521 attgattatc ctacttggta caactctcct aagtctcatt cctatcaatg ttgtaatcct
     2581 tacctttact cctgcaaaga atgcagttat ccagagtcca accggtacaa aagtgaagaa
     2641 cagcattaat agaacaatga tacctattgc aattgctaat gtgaaaataa gatccattct
     2701 aaatcctcct tacaaatatt tttgagcctt caaccttgac gaccttgaca tttgagtttt
     2761 cgggaatgta tcccgtctct gccaatgcat cgtacttctc gccatctatg atgataaaac
     2821 ccgaaggtcg aagttcagat agtacagttc cttccttgtc cagaaggtca ctcatggtgt
     2881 tggtgctgag atatcccctt gtgctttcaa gcttgttgct taatactatt ttgttcagga
     2941 acttgctctt gtaaccatgt tttaccataa caaccgccac caatgtagtt actataatag
     3001 caatgcttag ggacagaact gctgttgcca ggtcctgcat ggccaacact gtacccgcta
     3061 ttacgaatat caatccacct atgccaggta gtccaaaacc tggtaccaca ccctcaataa
     3121 ccaagaggcc cagacccaca acaaatagga tcagggatgt ccagtttgaa ttgcctgcca
     3181 gaatattacc accgaagtaa agtccaaaac ccatcaaaga gattgtacct cccaatccaa
     3241 aaccaggagt caggacctca agaacaagac ccacaaatcc cagagtcaat aggagggtac
     3301 tgatgtatgg gttggaaata tattttgaca gctttacctg aaggccctct tccataacct
     3361 ccacctgtga tgcctcaaat ccaaaatgct cgagaattac attgtaatca cttgacagaa
     3421 catctgcaag tcctagctgg tatgcctcct gtgcagtaag gttaaggagt ttccccccgt
     3481 ctattacacc ttctatggct atgtcgctgt cggccatgga ttctaccaga agggcgtccc
     3541 tgcccctgta ttgggctgta tctcttagaa cccctctcca cattgaaagg atcttttctg
     3601 tattgggtat ggtctcagcg gaaccaatca cggcattctc cgacattgct atgtgttctc
     3661 cagctattgt gaccagtaca ccagcagatg cggccttgtt gttcacaagg gttattgttg
     3721 gaatattggt cccgattatt acatccttta tcttgattgc ctcatcgaca acccctccgt
     3781 aggtgtcgat ttcaaatatt atggcctcta catcgggctc attgttaagg tcgtttacca
     3841 cgtccctcac atagttgtga gttgcgcgat tgatctctcc ctgtatgggg acaacataaa
     3901 cctttccagg gctttgtgcc tgggcgctgc cagatgatat aaaaataaca aatacgatta
     3961 taaaaaatgc cagaaatctt attttcttca cattttcacc tccaattatg tcctactttc
     4021 aatgtatacc cacaatagtt gaaagatagc aaaaaaacct gagtttaact caggttattt
     4081 aagcaatttt ctcacggccg tatttaccat gtttccatct gcccttcctt taaccttggg
     4141 cattgtggct ttcatgatca gacctatatc tttaattgag gtggctccaa tttcttcaat
     4201 tgtttcctta actatccttt ccacctcttc ttcattcatc tgttcaggca gatactccaa
     4261 cagaatctta atttctgact cagttgtatc cacgagatcc tggcgcgagc cttttttgaa
     4321 ttcctctatg gccatcttct tttccttaag ctgttttgca acaatctcaa ggatatcttc
     4381 atctgtcagc tcagttcttt catctacttc cctctgcttg acagctgccc taaccatagt
     4441 tacagtattt ttccttatag tatccttgtt tttcatggaa actttaaggt cttccatgag
     4501 tttatcttta agggacatta gttcaccttc ttattaatac ttggaatgtt tctttcttct
     4561 tgccgcttca gatttcttct tacgctttac gcttggtttt tcataatgct ctctttttct
     4621 tacctcacct ataactcctg atctggcaca tgatctttta aatcttttaa gagcgttatc
     4681 aagagattcg ttttcaccaa ctttaatttc tgtcatcgat cctttccctc ccctcacaac
     4741 tacaagagtg ttacaatact gctatgagta tggcttgcac actaaacaat tatacattat
     4801 cccgctaatt atttcaactg tttttaacct ggtggccata acatttctct ttttcctaaa
     4861 acatggaaat gaatgtggtc cacagactga cctccatcag gtccacaatt gttcaccacc
     4921 ctatagccat tctcaattcc ctggtctttg gccaacttgg gaatcaacct gaaaacgtgg
     4981 gaaatcaggg tatcgtgctc ctccgtaata tagtccaggg acttgatatg caccttgggc
     5041 acgactaaaa agtgtaccgg ggcttgtggg ttaatgtcct tgaacaccac aacaaattca
     5101 tcctcatgta ccagatcact tggaatttcc ttctttgcaa tcttacaaaa taaacagtcc
     5161 ataattaaca cctccccata tccctaataa gtaattcata ttaaggttcc atgaataaga
     5221 gaacccttaa cagcctctaa tcttgtatcc ctgatttctc cgatcaggtc caccggtgat
     5281 ggcgtcctta ccctgatgta gtttgtggta taaccttcat atacattttc gtccctcttg
     5341 gtgtgctcct catagaggac ttcctttgca ccttcaaggt atctattgtt gaactcctct
     5401 gacagccggt ctcccaaagc tataaggatc tcacttctct tgtttttgat ttctccatga
     5461 acctggtctt ccattgttgc agcaggcgta ccctttcttg gagaatactt gaatacatgg
     5521 attctggaaa atccaatttc ctccacaaag cgcagggtct cattgaactc atcctcagtt
     5581 tccccaggaa agcctacaat aatatcagtg gtgatacccg catcaggcag gtatttcctt
     5641 attaggtcca ccctgtccct gtactcttgt gttgtgtatt ttctgttcat cctttccagg
     5701 acagaatcag atccactctg cagtgacaga tgaaaatgat cacagatttt cgggcttttg
     5761 gtcataatct ccataaagtc atcatctatt agagttggct ccagggaact tagtcttatt
     5821 ctttcaatac cctcaatcag gctgatttta tcaattactt gacccaggga ttccttcttg
     5881 aggtccttcc cgtaggatgc aacgtggatt ccagtcagca caacctcctt gaagcctgcc
     5941 ttggccaggt tttcaacctc attttcaata tctgcaaact cccggcttct aattggtccc
     6001 ctggcatagg gaattatgca gtagctgcag aattgattgc aaccatcctg gattttcaca
     6061 aatgccctgg ttctggactt cagttcgtct atattgatct tttcgaactc cttgtaggtc
     6121 ttaacagatc tgacaatgtt tattctccgc ttgtggtttg aagcctcctc acaaaggtcc
     6181 acaatcctgt gtctctcact tgttccgatt atcacatcca caccctggat tttttcaacc
     6241 tcttcaggag acacctggga gtagcaacct actactgcta caattgctcc agggttgttt
     6301 ttcttggact ttcttatgaa ctgtctcgat tttctgtctg aaagatttgt aaccgtacag
     6361 gtatttataa catatatatc agaatagcca tcttcctcca caattgtgta gccttttttt
     6421 tcaaacattt cctccatagc ctcggtttcg tactggttaa ccttacaccc caaggtatgg
     6481 aatgccactg aattcattaa atcactccta aatctccaaa ttcatattga acaatggcac
     6541 aagccactat tcccgctgtt tcagtcctaa gtatcctcgg tcccagggac acaatccttg
     6601 cacccagcct gaccagcatg tctatctcat cttcctcaaa gcctccttca ggtccaataa
     6661 caatattgac cgtcctgggc acttccaatg acctgaggac atccttgatt ccattgtctt
     6721 ctgatgcctc atagggcact atggtaatac cttccttaag gtaggcttcg atttcagaca
     6781 tatcaatagc ctgatggacc tttggtatgg tgtcccgctt tgattgcttg gcagcttcat
     6841 ggacaacaga ctcccacctt tccagtttcc tgtcctgttt tttttcaccc tttaacctaa
     6901 ccaccgtccg atttgtcaac agtggataaa atgatgatac accaagctct gtggcttttt
     6961 gaaggatggt ctccatcttt gtgcttttgg gaagtccctg aaacaaattg attttgattg
     7021 aagattcgtg tcctctctct aaaggctgaa ctagctccgc aaccataata ttcttgccaa
     7081 tctcaattat cgaacacagg taggccttcc cctcaacaac aatctccagc ttttcacctg
     7141 atcccattct caagacatct ctagcatgct tgacatcaga tccttgtatt cttatacgtc
     7201 ccgctccatc tatttcatta tcagtaaaaa acctatgcat catatcacca ttctagatgt
     7261 aactgccgcc cagccattca taagttcaac atcgacaatt tcataaccgc tgctcatgag
     7321 ggcttccttt accttgtcca gcttgctttc tattataccg gaagcaatga agataccatt
     7381 atcatttaaa tgattcctaa gtcccggaac caggtcaact attatttctg caataatatt
     7441 ggccactatg acatctgcct tttcctcaac catatggagc agattgccct gctcaacctg
     7501 gattgtatca tccacctgat tgattattgc attttccttg gtgaccttga tacacattgg
     7561 gtccaggtct atggcaagaa ccctgccggc tccgagcttg gcagcaatta tacccaatat
     7621 tccacttcca cagcctacat cgaagactgt cgaggttgac tccacattcc tctccaaagc
     7681 tttggcgcac atgacggttg tttcatggga tccggttcca aaggccatac caggatcaag
     7741 ctcaatgact atgtcgtccc tttcaggctg atactcctcc caggaaggtt ttatgactat
     7801 atttgcacca atctttgttg tcttgtaatg gatcttccaa ttctcagccc aatcgctttc
     7861 atccaccttg ctggtgagga tttctcccaa tccctgattt ttgatccttt cccttatctt
     7921 ctcaagaatc ccttccaggt cctcagcctc tgaatagtat gcctttatga ttattccatc
     7981 atccttcttt ttcagtagct ctggctctat gaaatcccat gcatcctttg ccttgtcaag
     8041 ctccacaatg tcattgggat cctctattgt caatcctgta gcaccattgt catataggat
     8101 actgcttacc aggtcctcat tttcaggctt tgtcttgatt ataacctcca gccagttcat
     8161 cggatcacat ccttatctat tattcaacat attataccct aaaatcctac tcttttccag
     8221 tatgcaaaaa gcaaccaccc tggtggttgc tcccaactgg ctccaagcca gacttattgt
     8281 atagttctac tgctgaaatg catccttgac cttcttgaaa aaacccttct tttcaggagt
     8341 cttataattc tctcccattt ccttggaata ctccaggaga agctccctct gcttgtctga
     8401 aagatccttt ggtattatca cttcaactgt aaaaatcagg tcacccttgc caagtcccct
     8461 gacatttgcg acgcccttgt ttcttagctt gaatctgctt ccggatgggg ttgcagcagg
     8521 aagagtgtag acctctgttc cctacagtgt aggtatctct atttccgacc ccagggttgc
     8581 ctgggcaaat gttattggga ctgttacata gatgtttgtt cccctcctgg agaacactgg
     8641 gtcttccttg acggaaatat acagataaag gtcaccaggt ggtccaccct tttctccgct
     8701 gtgaccctca cctctgacag tcatcaccga atcactgtca acacctgctg ggatattgac
     8761 attgatcttt ctggtcttga tttccttgcc actgccctgg caggtttcac atttttcctc
     8821 aaaggtttca ccagtaccat tgcactggtc gcatacgcct acctgaacaa actgcccaaa
     8881 gggagtctgc tgtgtatacc ttacctctcc tgaaccatgg cattttgagc aggtattctt
     8941 gtcagttcca ggttttgcac cgctgccttc acatgtgcca caggtttcat accttctgac
     9001 ctgaacctct ttctccaccc caaaagctgc ttccctgaac tcaagagtta cgctttgctg
     9061 tatatcggct cctctggctg gaccggacct tcttctccct gtacttcttc caccaaagtc
     9121 accaaacaca tcaaacaggt catcaaatat atccccaaag cctccaaagt ctcctccgcc
     9181 accctgtgga tcaacaactg catgaccaaa cctgtcgtaa cgggaccttt tttcatggtc
     9241 gctaagaact tcataggctg cattcacttc cttgaaattc tgttcagcct ctgcatcgtt
     9301 tggattaagg tctgggtgat
//
LOCUS       DASRQF010000329        11099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_1810526, whole genome shotgun sequence.
ACCESSION   DASRQF010000329 DASRQF010000000
VERSION     DASRQF010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 11099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11099
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1810526"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1002
                     /locus_tag="VFD79_08795"
     CDS             91..1002
                     /locus_tag="VFD79_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968903.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HZH94187.1"
                     /translation="MNDGKRYNNLSGYLRDTFGKKVAKLSIDGGFSCPNRDGHKGDRG
                     CIFCGEMGAGEFASRLGSVKDQMDEQIRLLGSKWNTDSYIAYFQSFTNTYASVERLKE
                     VFEPVLDHPGIVGIAIATRPDCLPKEVLDYLSQLNERTFIWVELGLQTVHEDTAKLIR
                     RGYPLETYKNAVEELAKRGIRVVTHLIVGLPNESREMILESARYVGETGTWGIKIHSL
                     YIQRDTDLYTMFLEKPFPLMTMEDYVDTISEIISIIPKEMVVHRITGDGDKSLLHEPK
                     WSMNKLRVISSIDKRLKEMDISQGCAL"
     gene            1097..2065
                     /locus_tag="VFD79_08800"
     CDS             1097..2065
                     /locus_tag="VFD79_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH94188.1"
                     /translation="MKNKKWMLILLSLVMVMALAACGNNEEEVQEPEAEPPVQEEPVQ
                     EAEEDEKQMIMEEFRIIVEDERASAAVIKEFMDENLPVLGELEGNHMFDNLENALKRE
                     IGDIDRDLMELDPEGKLNELMGNSGQLGLEMIAQIEDEDLKAAVENAYDNHYKLISRE
                     GQVEAVIDYSSLKEYQEKVTDEWKEYIDIMAVESDQAPFSDGSLMITFDQLAERILSI
                     ENYLNRYVSGPRQEELLELYENRLTAYYKGLPNTPIAAYDSGEILDNVYKSYENTAAN
                     EGYVTSSMIGEYRIAIRDNNMIVDDSIMALADQYIDESVRVLTEFK"
     gene            2156..3385
                     /locus_tag="VFD79_08805"
     CDS             2156..3385
                     /locus_tag="VFD79_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HZH94189.1"
                     /translation="MSELIDNRSKKPYDKEKLEKLKEVIKELHEGKTVQEVKAKFADV
                     LEGTSPLDISRMEVELVKDGLPIEEIQNLCDVHAEVFKGSLEEIHHPDEVPGHPVYTL
                     RQENMALTNIMEEVIPEDLERFMEDDSDDNRFKLVEDINLLWDIDKHYSRKENLIFPY
                     LEKAGITAPPQVMWGVDDEIRDKIKAAKKELVEYSGDKDLVKEKILAATEQAIDMIFK
                     EESILFPMTLDTLTEDEWIKVYEGSEEIGYAVIPPQKEWELKRVNVVKKEEDKGITES
                     GFVRFETGILSSKELELMLNHIPGDFTFVDKDNIVKYFSQGKERIFARTKAIIGRTVE
                     NCHPPKSAHIVEDLVNDFKAGKKDSESFWIQMGEKFILIQYFAVRDENGEFMGTLEHS
                     MDIAPLRELEGEKRLMS"
     gene            3434..3994
                     /locus_tag="VFD79_08810"
     CDS             3434..3994
                     /locus_tag="VFD79_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family glyoxalase III"
                     /protein_id="HZH94190.1"
                     /translation="MNKVLVMLANGFEEVEALTVVDYLRRTGIEVKMVSTEKGLEVEG
                     AHGIKVLGDLMIDDIKAPLNYRGIFIPGGLPGATNLRDDSRVTDLVKTLADGGKMIAS
                     ICAGPIVLCKAGVIDGKKVTSYPGYARELVGAVYKEDLVVRDGNIITSRGPATAVYLA
                     MEIIRYLQGEEKLRELRKAILLDLIK"
     gene            4152..5096
                     /locus_tag="VFD79_08815"
     CDS             4152..5096
                     /locus_tag="VFD79_08815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HZH94191.1"
                     /translation="MKKQFIAVFIASFILFGVFYGILANTIFRNDTVVATDPDPLETE
                     NVTEDPVEEEKNDILFLLMGVDAQDVTQSKGTRTDTMMVTSVDLDTGKISILSVPRDT
                     RTTIQGRQDKINAAHAYGGAEAAVEATANLLNIDLEYYVKVDYQIVQDVVNAIGGVTV
                     EVPFNMRYSDPTSKPPLNINIDKGLQTLDGKNAHDFLRFRHNNAMTVGYPDGDVGRTR
                     TQQYFMKELIKQTIKPRNLLKLPSLVETYFDNVETNIPIRVMLRGALAANKIDVESME
                     TETLPGAGSYIGGISYYIHDGALTEEVVRKMFGNYLVD"
     gene            5134..6144
                     /gene="fni"
                     /locus_tag="VFD79_08820"
     CDS             5134..6144
                     /gene="fni"
                     /locus_tag="VFD79_08820"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019034215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HZH94192.1"
                     /translation="MRRVRKKEHIENYLKTSYRGDTLLGDVFLTHNALPNLNFDDIDT
                     STEFLGKRINNPIMINAMTGGSDFSQEINRDLATLAKELGIPMAVGSETIALEDDESV
                     QSFKVVREIIGPEGIVIANLSGIASVDEARRAIEIVQGDAIQLHLNLAQELVMEEGDR
                     DFLGVINNIEEVVKNVDVPLIVKEVGFGISHDVGKRLYDAGVRYIDVSGYGGTNFIEI
                     ENLRSHEVDFSELYSWGIPTAASLVQCRRLPEDVSLIASGGLRNSMDIVKSIVLGADI
                     AAVSGELLAYLVHGGYANAKDYLESNLYKLRVLMMLLGKGNIQELKKTEYKLTGKLKE
                     IV"
     gene            6199..7176
                     /locus_tag="VFD79_08825"
     CDS             6199..7176
                     /locus_tag="VFD79_08825"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HZH94193.1"
                     /translation="MKHKILDRLHEEKGFLSGEKLSEEFGLSRTAIWKHVKALKADGY
                     QIESVTRKGYKLVSSPDIVNYDEIKEILHTKIIGGKMIHFNSLESTNKTAKEVAVVND
                     EGTVVVAEQQTKGKGRLGREWISPNRKGLYFSVILKPDTDPTKVAKLTLLGAASVNRG
                     LLDLGIQSLIKWPNDIVINGKKVAGILTEMSCELGTINHIIIGIGINVNQSKEEIPYD
                     LRDRATSLLMESGKAINRKELFAAILNRLDQFYMEFLQDENMDGVLEICRENSAVIGK
                     DVLVIQGGNSRKGHVVGINHSGELQVRFDTGLETVFSGEVSIRKEDEYI"
     gene            complement(7199..7741)
                     /locus_tag="VFD79_08830"
     CDS             complement(7199..7741)
                     /locus_tag="VFD79_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HZH94194.1"
                     /translation="MKTTTREITLISLFSALTAIGAFISIPLGPVPITLQTLFVLLAG
                     FLLSPRSAALSQIVYISIGLVGLPIFSGFTGGLQAVLKPSFGFLVGFVVASFLASLIS
                     KKHRSARGLLIAGAVATITMYIIGIPYMAFILNHVMDGGFSLMAILNMGLLLFLPGDL
                     LKLIIATYLGNRLGKVLQKK"
     gene            7886..8260
                     /locus_tag="VFD79_08835"
     CDS             7886..8260
                     /locus_tag="VFD79_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZH94195.1"
                     /translation="MRILISNSSDEPIYTQIKDQIKDQIIRGELDAGVQLPSIRGLAK
                     DLQISVITSKRAYEELEREGFVETIPGKGSYVAPQNKELIREQRLRIIEGSLMDVVRE
                     GKLLGLDYKELEEMLRILFEEE"
     gene            8263..9114
                     /locus_tag="VFD79_08840"
     CDS             8263..9114
                     /locus_tag="VFD79_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZH94196.1"
                     /translation="MLEVRNLSKKFKEGFHLDDISFTLEPGYIMGFIGPNGSGKTTTI
                     NLIMNLMKRDSGTVRIFGDDITENEREIKNRIGFVYDEAHFYDDLTLEQMKRIIAPFY
                     KEWNDSLYKDYIDKFELDSRMRIKKMSKGMKTKYSLALALSHNADLIIMDEPTSGLDP
                     IFRKEILDILYDIIQDENKSIFFSTHITTDLEQIADYITFVNKGRMVFSKPKDEIMEE
                     FKIVKGDSRNLIPDVRERFISLRETGYGFEGLTREATKLKDMLDDNTLVENASLQDIM
                     YYMRTEG"
     gene            9117..9752
                     /locus_tag="VFD79_08845"
     CDS             9117..9752
                     /locus_tag="VFD79_08845"
                     /inference="COORDINATES: protein motif:HMM:NF024741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HZH94197.1"
                     /translation="MGPLVMKDIRLIVSNRATQIFLLLYIPFLLIIAGYDFRAEYMYL
                     VVLVSGAYMITILSFSYEIGRKTNQLLLSLPVRRTDIVNAKYLFVFAAFIMSGVYSGI
                     YIGLIKHFLDSQLIAFDLSMLRNASILTLLFVSLVLPLFFGLPHKIANLLNVFVFVTL
                     FQIFGMGVGTLEGIFASPVFAGAGGLYGISAGILLLIISRQLSSFLFARRD"
     gene            9765..10433
                     /locus_tag="VFD79_08850"
     CDS             9765..10433
                     /locus_tag="VFD79_08850"
                     /inference="COORDINATES: protein motif:HMM:NF024741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HZH94198.1"
                     /translation="MLSLFKKDVMISRKPMMLIGVLVLIWTIPAFLVQNQIMAQVVFG
                     YMLFFQSYYLAIMLTQQDEAVKADLIINSLPVNRNLVVAERYLFFIIQPAAMTAFILV
                     FSRAVWSIPAPGFPQGQGFSIVGAMVVLPIIWIILTFYLPINYMSIGKIKVFNQLSFM
                     ALILLPGIASRFIKGAQMPSWFQGILEMPVGLVVASLIIVSMTSYFISYQFSKRIYRN
                     KDFY"
     gene            10542..>11099
                     /locus_tag="VFD79_08855"
     CDS             10542..>11099
                     /locus_tag="VFD79_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH94199.1"
                     /translation="MKRRVALILAIIMTFNMLMFTSCGKESASEQPKDGTQLMEDASP
                     EESLVDYIGASPFYTPSGLGTVYGDSEYISSRKIQIENIMSLSDVMVMNLEYFLALSD
                     ELQWEALANYRYFSELDPEVQMVYEIIRDKYTGQMIDTAQLLSHIRAMENKDNEDPYI
                     KAWNDYDLVQKRLELTALTFGRIMEI"
BASE COUNT         3537 a         1948 c         2796 g         2818 t
ORIGIN      
        1 ggagggagct gcctgggaca aaccacagga agtgaccgag aagatggtat cagccttggc
       61 ccaccttttt gtgggagagg aagaataata atgaatgatg gtaaaaggta caacaatctt
      121 agtggatact tgagggatac ctttggaaag aaggtggcaa agctatccat tgacggaggc
      181 ttcagctgtc caaacagaga cgggcacaag ggtgaccggg gatgtatttt ctgtggagag
      241 atgggtgcag gggaatttgc atcaaggctt ggatcggtaa aagaccagat ggatgaacag
      301 atcaggctcc ttggaagcaa atggaatact gatagctaca tcgcatattt tcaatccttc
      361 acaaacacct acgcctctgt ggaaagactg aaggaggttt ttgagccggt gctggaccat
      421 ccgggaattg tgggcatagc aattgccaca aggccggact gtctgccgaa ggaggtgctg
      481 gactaccttt ctcagctgaa tgagagaacc ttcatttggg ttgagcttgg ccttcagacg
      541 gtacatgaag atacggcaaa gctaataaga cggggctacc cactggaaac ctacaaaaat
      601 gctgttgagg agcttgcaaa aaggggaata agggtagtta cccacctgat agtaggcctc
      661 cccaatgagt ctagggagat gatcctggaa tctgcaagat atgttggaga gacaggcacc
      721 tggggaataa agatccattc cctctatata caaagggata ctgatctgta cacaatgttt
      781 ttggaaaaac cctttcctct aatgacaatg gaggattatg tggatacaat atccgagata
      841 atatcaatta tccccaagga gatggttgtt cacagaataa ctggcgacgg tgacaagtcc
      901 ctccttcatg aacctaaatg gagcatgaac aagcttaggg tcatatcatc aattgataaa
      961 agactcaagg aaatggacat ttcacagggc tgtgcactat aattgtaatt gaattgtaaa
     1021 gacttcccat atgggaggtc tcttttattg ggtatatagg ttttaaggga atgtttaatg
     1081 aaagtgaggt gcagacatga aaaataaaaa atggatgttg attttactat ctcttgtaat
     1141 ggttatggct ctggcagcct gtgggaataa tgaagaggag gtacaggagc ctgaagctga
     1201 accgccagta caggaggagc cggttcagga agctgaggaa gacgaaaaac aaatgataat
     1261 ggaagaattc aggataatag ttgaggacga gagagcatca gctgctgtca tcaaggaatt
     1321 catggatgaa aacctgccgg ttcttggtga attggaagga aaccatatgt ttgataattt
     1381 agagaatgca cttaagagag agattggaga cattgacagg gacctgatgg aacttgaccc
     1441 ggaaggcaag ctaaatgaac tgatgggaaa cagtggtcag cttggtcttg aaatgattgc
     1501 acaaattgag gatgaagacc ttaaggcagc ggtagaaaat gcctacgaca accactacaa
     1561 gctcataagc agggagggcc aggttgaggc agtaatagac tactcaagcc ttaaggaata
     1621 tcaagagaag gtaaccgacg agtggaagga atacatcgat atcatggctg tggaatcaga
     1681 ccaggcacca ttcagtgacg ggtcattgat gataaccttt gaccagctgg cagagaggat
     1741 tttgagtata gaaaactacc tgaacagata tgtatccggt cctagacagg aagaacttct
     1801 tgagctttat gaaaacaggc ttaccgccta ttataagggg cttccaaaca ctcccattgc
     1861 agcatatgac agtggggaga ttctggacaa tgtgtacaaa tcctacgaaa acacggctgc
     1921 caacgaagga tatgtaacat caagcatgat tggtgaatac aggatagcaa taagggacaa
     1981 caacatgata gtggacgatt ccataatggc acttgctgac cagtatatag acgagtctgt
     2041 aagagtgttg acagagttca aatagtagca gtcaataggg gccggatggc ccttttattg
     2101 tgttgtggtc cgaattaagg gtatatatgt taaagtttat gaaaggagtg gtacgatgag
     2161 tgaattgata gacaatagaa gtaaaaagcc atatgacaag gagaaactgg aaaagcttaa
     2221 ggaggtaata aaggagctgc acgagggtaa gactgtacag gaggtgaagg ccaagttcgc
     2281 cgacgttctt gagggcacat cgccactaga catatccagg atggaggttg agctggttaa
     2341 ggatggattg cccatagagg aaattcagaa tctgtgtgat gtgcatgcag aagttttcaa
     2401 gggatcctta gaggagatac atcatcctga tgaggttcct ggacatccgg tctataccct
     2461 ccgtcaagag aatatggcat tgaccaacat aatggaagag gtcattccag aagaccttga
     2521 aaggtttatg gaggatgatt ccgatgataa caggtttaag cttgtcgaag atataaatct
     2581 tctatgggac atagacaagc attacagcag gaaggaaaac cttatatttc catatcttga
     2641 aaaggctgga ataactgcac ctccacaggt aatgtgggga gtggacgatg aaatcagaga
     2701 caagatcaag gccgctaaga aggagcttgt tgagtactca ggcgacaagg acctggtcaa
     2761 ggagaagata cttgcagcaa ctgagcaggc tattgatatg atattcaagg aggagagcat
     2821 cctctttcca atgaccctgg ataccctgac agaggacgag tggatcaagg tgtacgaggg
     2881 gtctgaggaa attggttatg cagtcatacc ccctcaaaag gagtgggagc ttaaaagggt
     2941 caatgtggta aaaaaggaag aggataaggg gataactgaa agcggctttg taagattcga
     3001 gacagggata ctctcttcca aagagcttga gcttatgctg aaccatattc ccggagactt
     3061 cacctttgtt gacaaggaca acatagtaaa atacttctct cagggaaagg agagaatttt
     3121 tgccagaaca aaggcgatta tcggaagaac cgttgagaat tgtcatccgc ccaagagtgc
     3181 ccacatcgta gaggaccttg tgaatgactt caaggctgga aagaaggatt cagagagctt
     3241 ctggattcaa atgggtgaaa agttcatcct tatccagtat tttgcagtta gggatgaaaa
     3301 tggagagttt atgggaaccc tggaacattc catggatatc gcacccctaa gggaattgga
     3361 gggggaaaaa agattgatgt cctaggacct tggagggata ggttcgtgaa gttcagcaat
     3421 ggaggtgatt gaggtgaata aggttttagt tatgctggcc aatggttttg aggaagttga
     3481 ggctttgact gttgtggatt acctgagaag aaccggaatt gaagtgaaga tggtctccac
     3541 tgaaaagggt ctggaggttg agggagctca tggaataaag gttttgggag acctaatgat
     3601 agatgacata aaggccccct tgaattacag gggaatcttc attccgggag gacttcctgg
     3661 agcaacaaac cttagggatg attcaagagt aactgatttg gtcaagactc ttgcagatgg
     3721 tggaaagatg atagcttcaa tatgtgcagg cccaattgta ctgtgcaagg ccggagtcat
     3781 agacgggaaa aaggtgacaa gttatccagg ctatgccaga gagcttgttg gagcggtata
     3841 taaagaggat ctggtggtaa gggatggaaa tattataacc tcaaggggac cagctactgc
     3901 ggtttacctt gcaatggaga taatcagata ccttcagggg gaggaaaaac tgagggaact
     3961 gagaaaagcc atcctgttgg atttgattaa ataagtgaaa aggggctaag gcccttttct
     4021 ttttaggggg gtaagacagg ttaaaatgtc atttgtatcc aaattgtaat acaagaggct
     4081 ggataaataa ttgtaaatac accttaatta tagtattata taatgtagca taaaaacaaa
     4141 tgaggtgtat tatgaaaaaa cagtttatag cggtgtttat tgcctccttt atcctttttg
     4201 gagttttcta tggcattctg gcaaatacaa tattcagaaa tgatactgtt gttgctacag
     4261 atccagatcc attggaaacg gaaaacgtaa ctgaggaccc cgttgaagaa gaaaaaaatg
     4321 atatattatt cctgcttatg ggtgtggatg cacaggatgt gacacagtca aagggaacca
     4381 gaacagatac gatgatggtg acaagtgtag accttgatac cgggaaaata agcattctgt
     4441 cagtacccag agatacaagg acaaccatac agggaagaca ggacaagatc aatgcagccc
     4501 atgcctatgg tggtgcagaa gctgctgtag aagctacggc aaatcttctg aacattgacc
     4561 tggaatacta tgtaaaggtt gattatcaga tagttcagga tgttgtaaat gcaattggtg
     4621 gagtaacagt tgaggtgccc ttcaacatga gatacagtga cccaacatca aaacctccac
     4681 tgaacataaa tatagacaag ggtctgcaaa cccttgatgg aaagaatgca catgatttct
     4741 tgagattcag gcacaacaat gccatgaccg ttggctatcc cgacggtgac gttggaagga
     4801 ccagaacaca gcaatacttt atgaaggagc ttataaaaca aacaattaag ccaagaaacc
     4861 ttcttaagtt gccttcactg gtggaaacat actttgacaa tgttgaaaca aacattccaa
     4921 taagagtaat gcttagggga gcactggctg caaacaagat tgacgtggag tccatggaga
     4981 ctgagaccct gcctggtgca ggaagctata taggtggaat cagctactat atccacgatg
     5041 gagccttgac agaggaagtt gtcaggaaaa tgttcggaaa ctacttagtg gattgatttt
     5101 tttgccacaa gaataattaa aagaggtgta attatgcgaa gggtaagaaa gaaagaacat
     5161 attgaaaact atctgaagac ttcctacagg ggagacacat tgctggggga tgtgttttta
     5221 acccacaatg cgttgccaaa tcttaacttc gacgacatag ataccagtac ggagtttcta
     5281 ggaaagagaa taaacaatcc catcatgata aatgcaatga ctggaggttc tgacttttcc
     5341 caggaaataa acagggacct tgcaacactt gcaaaggagc ttggaattcc catggctgtg
     5401 ggatctgaga caattgcctt ggaagacgac gaaagcgtcc agtcctttaa ggtggtaagg
     5461 gagatcattg gtccggaagg aattgtgatt gccaacctaa gtggaatcgc ttctgttgat
     5521 gaagcaagac gagcaattga aattgttcaa ggtgatgcaa ttcagcttca tcttaacctt
     5581 gcccaggaac ttgtcatgga agagggagac agggattttc tgggtgttat aaataacatc
     5641 gaagaagtgg taaagaatgt ggacgtaccc cttatagtaa aggaagttgg ctttgggata
     5701 tcacacgatg taggcaagag gctttatgat gctggtgtga gatacatcga tgtttcaggt
     5761 tatgggggta ccaacttcat tgagatagag aatcttagaa gccacgaggt tgatttttct
     5821 gagctgtatt catgggggat accaactgcg gcatcactgg tgcagtgcag aaggttacct
     5881 gaggatgtca gtctgattgc ttccggtgga ctaagaaact ccatggatat tgtcaagtcc
     5941 atcgttcttg gtgcagacat agctgctgtg agcggagagc tgctggctta cctggtacac
     6001 ggaggctatg caaatgcaaa ggactatcta gaaagcaacc tttacaagct tagggtattg
     6061 atgatgctgc ttggaaaggg gaacatccag gagctaaaga aaacagagta taaactaaca
     6121 ggaaaactta aagagattgt ctagattaga gagggttcca gagaggaccc ttttcttgta
     6181 caaaagggag ggctgcaaat gaagcacaag attttggata gactgcatga ggaaaaaggt
     6241 ttcttgtctg gagagaaact ctctgaggag tttggtctga gcagaacagc catatggaag
     6301 catgtcaagg ccttgaaagc cgacggttac cagattgagt cagttaccag aaaaggatac
     6361 aagcttgtgt catcccctga tatagtcaac tatgacgaga taaaggaaat ccttcatacc
     6421 aagattattg ggggtaaaat gattcatttc aactcccttg aatcaacaaa caagactgcc
     6481 aaggaggttg cagttgttaa cgacgaaggc actgttgttg ttgcagagca gcagaccaag
     6541 ggcaaggggc gcctgggacg ggagtggata tctccaaaca gaaagggact ctatttttct
     6601 gttatcctaa aacccgatac ggaccccaca aaggttgcca agctaaccct tctaggtgca
     6661 gcctctgtaa acagggggct tcttgatttg gggatccagt ccctaataaa gtggccaaat
     6721 gatattgtaa taaacggcaa aaaagtcgct gggatactaa ctgaaatgag ttgtgagctg
     6781 ggaaccatca accatataat catcggaatt ggcattaatg taaaccagtc aaaagaagaa
     6841 atcccttatg atctaaggga tagggccaca tccctcctta tggaaagcgg caaggccata
     6901 aacaggaagg agctgtttgc agctatcctg aacaggcttg accaattcta tatggaattt
     6961 ctacaggatg aaaatatgga tggagttctt gaaatatgca gggaaaattc tgcagtaatt
     7021 ggaaaggatg tccttgtgat ccaggggggc aactcaagga aaggtcatgt tgttggcatt
     7081 aaccacagcg gtgagcttca ggttaggttt gacacaggtc ttgagacagt gttctcaggg
     7141 gaggtttcaa tcagaaaaga ggatgaatat atctagcatg ctggaagtcc agcatgcctt
     7201 attttttttg tagaacctta cctagcctgt tacccaggta ggtggcgatg ataagcttca
     7261 acagatctcc cggaagaaaa agtagaagtc ccatgtttag aatagccatc aaggagaaac
     7321 caccgtccat tacatggttt aggataaaag ccatataagg gattccgatt atgtacatgg
     7381 taatggttgc aactgctcct gcgattaata gaccccttgc ggagcgatgt ttttttgata
     7441 tcaatgaggc aaggaaacta gccacaacaa agcccaccag gaatccaaag ctgggtttaa
     7501 gtacggcttg caatccccct gtgaaacctg aaaaaatagg tagtccaaca agaccgattg
     7561 atatgtatac tatctgtgat agtgcagcag aacgagggct taaaagaaaa cccgccagaa
     7621 ggacgaaaag tgtttgcaat gttatgggca caggacccaa ggggatggaa ataaatgcac
     7681 caattgcagt caatgctgaa aaaagtgaga tcaaggttat ttcacgtgtt gttgttttca
     7741 tataccctcc tattgtcaac ctaaattcaa taatattagt ttacaattaa aacaaaataa
     7801 aatcaagtaa aatattgaca agtaatatat tattgtctat actgtatata cacgatataa
     7861 acagtataga cggtggaggt tgaaaatgag aatactaata tccaatagct ccgatgaacc
     7921 aatttatact cagataaaag atcaaataaa ggatcagatc ataagaggtg agctggacgc
     7981 aggagtccag ctgccctcca taagaggtct ggccaaggac cttcagattt cagtgatcac
     8041 aagcaagagg gcatacgaag aactggagag agaaggattt gttgagacga tacccggcaa
     8101 gggaagctat gttgcgcctc aaaacaagga attgataagg gaacagaggc tcaggatcat
     8161 tgaaggctcc cttatggatg tcgttcggga aggtaagctt ttgggcttgg actataagga
     8221 gcttgaggaa atgttaagaa ttctttttga ggaggagtag aaatgcttga agtaagaaat
     8281 ctatcaaaga aattcaagga aggttttcat ctggatgaca tatcctttac cctggagcct
     8341 ggatatatca tgggtttcat aggtccgaac ggttcaggaa aaaccaccac aattaatctt
     8401 atcatgaacc taatgaagag ggattcgggg acggtaagga tttttggaga tgatataact
     8461 gagaatgaaa gggaaataaa gaacaggatt gggtttgtct atgatgaggc ccacttctat
     8521 gacgacctta ccctggagca gatgaaaagg ataatagctc ccttctacaa ggaatggaat
     8581 gatagcctat acaaggatta cattgataag tttgaacttg attcaagaat gagaatcaag
     8641 aagatgtcaa aagggatgaa gacaaaatat tctttggcac tggccttaag tcacaatgct
     8701 gatctgataa taatggacga accaacctcg gggcttgatc ccattttcag aaaagagatt
     8761 ctggatattc tatatgacat aatccaggat gagaacaaat ccatattctt ttcaacccac
     8821 atcacgacag accttgagca gattgctgac tatatcacct ttgtaaataa agggagaatg
     8881 gttttttcaa agcccaagga tgagattatg gaagaattca agatagtaaa gggtgattca
     8941 agaaacctta ttcccgatgt aagggagagg ttcataagtc ttagggaaac cggatatggc
     9001 tttgaaggtc ttacacggga ggcgacaaaa cttaaggaca tgttggatga taacacattg
     9061 gttgaaaatg catcactgca ggatatcatg tactacatga gaacggaggg ataaagatgg
     9121 gtccacttgt tatgaaggat atcaggttaa ttgtatcaaa cagagccacg cagatatttc
     9181 tgcttctcta catccctttc ctgcttatta ttgctggcta tgactttagg gcagagtata
     9241 tgtatctggt ggtccttgtc tccggagcat acatgataac aatactgagc ttctcgtacg
     9301 aaataggaag aaagacaaac cagctgctcc tttcactacc tgttagaagg actgatattg
     9361 tcaatgcaaa atatctcttt gtgtttgcag catttataat gtcaggagtc tattctggaa
     9421 tatacattgg gctaatcaag catttcctgg atagccagct gatagcattt gacctttcaa
     9481 tgctcaggaa cgcatcaata ttgacgctcc tgtttgtaag tcttgtacta ccgctgttct
     9541 tcggtctgcc acacaaaatt gcaaaccttt taaacgtatt tgtatttgtt accctgtttc
     9601 agatatttgg catgggcgtg ggaactctgg aaggtatttt tgcttctcca gtcttcgctg
     9661 gagcgggtgg actctatggt atatcagcag ggattcttct tttgataata tccaggcagc
     9721 tatctagttt tctatttgca agaagagatt aggggttgat tataatgcta tcgctattta
     9781 agaaggatgt tatgatatct agaaagccca tgatgcttat aggtgttttg gttctaatct
     9841 ggacaatacc ggcatttctt gtacagaacc aaataatggc acaggttgtg ttcggctaca
     9901 tgctgttttt tcagagctat taccttgcca tcatgctgac ccaacaggat gaggctgtta
     9961 aggctgacct tatcataaac agtcttccag tcaacaggaa tctagttgtg gcagaaagat
    10021 acttgttctt tattatccag cctgctgcaa tgacagcctt tatccttgtg ttttcaaggg
    10081 cggtatggtc cataccggca cctggatttc cccagggaca gggattctca attgtaggcg
    10141 ccatggtggt actacccata atatggataa ttctgacctt ctatctgccg ataaactata
    10201 tgagcatagg gaaaataaag gtattcaacc agctgtcatt tatggcactg atacttttgc
    10261 caggaatagc ctcaagattc attaaaggtg cccagatgcc aagctggttt caggggattc
    10321 tggaaatgcc tgtggggctt gtggtagcta gtctgataat tgtatcgatg acaagctatt
    10381 tcatatccta ccagttttca aaaagaatat ataggaacaa ggacttctac tagggatgga
    10441 ggtccttttt cataggtaaa atcatatata atctagtctt taggtggata aagtggtaaa
    10501 atgaatacaa taaaatccta tgtccaaaag ggggctccgc aatgaaaaga agagtggctt
    10561 taatattggc aataataatg acatttaata tgcttatgtt tacatcctgt ggtaaggaat
    10621 cagcttcaga acagcctaaa gacggaacac agctaatgga ggatgcttcc ccagaagaaa
    10681 gccttgttga ctacatcggg gccagtccct tctacacccc aagtggttta gggaccgtat
    10741 atggggatag tgaatatata agtagcagga agatccaaat tgagaacata atgtcactgt
    10801 cggatgtcat ggttatgaat cttgagtatt tcctggccct gtcagatgag cttcaatggg
    10861 aggcgcttgc aaactacagg tatttcagcg agcttgatcc tgaggtgcaa atggtttatg
    10921 aaatcataag ggacaagtat acagggcaga tgatagatac agcacagctt ctgtcccaca
    10981 taagagcaat ggagaataag gataatgaag atccatacat aaaggcctgg aatgattatg
    11041 accttgttca aaaaaggctg gagctgacag ccctaacctt tggaaggata atggagatt
//
LOCUS       DASRQF010000330         2007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_819225, whole genome shotgun sequence.
ACCESSION   DASRQF010000330 DASRQF010000000
VERSION     DASRQF010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819225"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VFD79_08860"
     CDS             complement(<1..231)
                     /locus_tag="VFD79_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZH94200.1"
                     /translation="MAFKEMMDYMKNEMKFPTKDLNDLPTSTKTFEGGAHYRMEITGI
                     ESAKNFENLVKEIDKQKVVIHRVIGMVGGSALI"
     gene            complement(294..1142)
                     /gene="panB"
                     /locus_tag="VFD79_08865"
     CDS             complement(294..1142)
                     /gene="panB"
                     /locus_tag="VFD79_08865"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HZH94201.1"
                     /translation="MPRKKSVLDLYKMKKSGEKVAWITAYDYPTAMFAEAAGMDMLLV
                     GDSLGMVTLGYSGTIPVTMEDCISHCQAVRRGAPNTFIIGDMPFGAYQSSDEQAVDNA
                     VRFLKEADVDAIKLEGGIRVQSRIRAITDAGIAVFGHIGLTPQSSGQLGGFKAQGRTV
                     ESAKGVIEDALAVQEAGAMMLLVEAVPPEVTKFIADLLEIPVYSIGAGDCDGQLLISS
                     DMVGKFQAFTPKFVKKYANVAEIETNAYKEYVKDVKEGVFPSDEYVYHVTDPIEDFDE
                     LFKEYK"
     gene            complement(1602..>2007)
                     /locus_tag="VFD79_08870"
     CDS             complement(1602..>2007)
                     /locus_tag="VFD79_08870"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZH94202.1"
                     /translation="LRCLHPDTREIQERMREIVDGISKVHRTTFSLEFKCGNDLLYND
                     EGMTGLAEHVAEGIVGREKLITSDISVMLGEDFAEFSKMVPSTFIFLGTADRDKGTDY
                     PHHHPKFNIDEEVLPLGVEVLVGTALRYLGES"
BASE COUNT          501 a          500 c          338 g          668 t
ORIGIN      
        1 tataagggcc gatcccccta ccataccgat taccctatgg attacaacct tttgcttgtc
       61 aatctccttg acaaggtttt caaagttctt ggcactttca atacctgtga tctccattct
      121 atagtgagct ccaccttcaa aagtcttggt tgatgtaggt aggtcattca gatccttggt
      181 aggaaatttc atttcattct tcatatagtc catcatttct ttaaaagcca tatttgttgc
      241 ctccttttag taggttattt taattgtagt aagcttcctg ttatctatcc tgattatttg
      301 tactccttga acagttcatc aaaatcctct attgggtctg ttacatgata aacgtattca
      361 tctgatggga atacaccttc cttaacgtcc ttgacatact ccttgtatgc gtttgtttca
      421 atttctgcaa cgtttgcata cttcttaacg aattttggtg tgaatgcctg gaattttcca
      481 accatgtcac tacttatcag cagctggcca tcacagtctc cagctccaat tgaataaacc
      541 ggaatctcca gtaggtctgc aatgaacttt gtaacctctg gtggtacagc ctcaacaagc
      601 agcatcattg caccggcttc ctgaactgca agtgcatcct cgataacgcc tttggcgctc
      661 tcaactgttc ttccctgagc cttgaatcct cccaactgtc ctgagctttg aggagtcagt
      721 ccgatatgac caaagacagc tattcctgcg tcagtgattg cacggattct actttgtact
      781 cttatgccac cttccagctt tatggcatca acatcagctt cctttaggaa tctaactgca
      841 ttgtcaactg cctgctcatc tgagctttgg tatgcaccaa atggcatgtc accaatgata
      901 aaggtgttgg gagctcctct tcttacagcc tgacaatgtg aaatgcagtc ttccattgta
      961 acaggtatgg ttccgctgta tccaagagta accattccca atgagtctcc aacaagaagc
     1021 atatccatcc cggccgcttc tgcaaacatg gctgtcggat agtcatacgc tgtgatccaa
     1081 gccaccttct caccggattt cttcattttg tacagatcca atacactctt ctttcttggc
     1141 atcctaaatc tcctcctctt cttatgtaac catctactat caacggtccg ttcagaattt
     1201 ccctttgcaa tctcggttac atgattacaa gtttaaatca cctgatccaa aagtctttct
     1261 gttttattag aacgttgttc tacttttgat actatagtac attaatttga ctgtcaagaa
     1321 aatttttcag aaatttgaga attgactttc ttaattatgg cctatttttt ccaatatggg
     1381 catattgctt gtatccagct gatatcaagg attttatgaa ttttttttca tataatgtga
     1441 ccaaaaccta cgacattatg atcgtcatct gtatttttgt agaatacaat taaaattgga
     1501 atgctattcc atttgttccc ttttcccaaa aaaaccagag gatacttctt cctctggttt
     1561 tagtttgacc acttattcaa ttcacgtgca tcctgttttc cctagctttc ccccaaatac
     1621 cttaatgcag tgccgactag gacctccacc cccaagggca gtacctcctc atcaatattg
     1681 aacttggggt gatggtgggg gtagtctgtt cccttatccc tgtctgcagt tcccaaaaat
     1741 atgaaggtgc ttggaaccat cttggaaaat tcagcaaaat cctcccccag cataacgctt
     1801 atatccgaag tgatcagttt ttctctcccc acaatccctt ctgcaacatg ttcagccaga
     1861 cctgtcatgc cctcatcatt gtacaaaaga tcgtttccac acttgaactc caatgagaaa
     1921 gtggttcggt ggaccttgga tattccatca acgatttccc tcatcctttc ctgtatctca
     1981 cgggtgtccg ggtgcaggca tctcaag
//
LOCUS       DASRQF010000331        10985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_72418, whole genome shotgun sequence.
ACCESSION   DASRQF010000331 DASRQF010000000
VERSION     DASRQF010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 10985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10985
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72418"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VFD79_08875"
     CDS             complement(<1..311)
                     /locus_tag="VFD79_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HZH94203.1"
                     /translation="MNRVVITGLGAITPVGSGKEEFWNSIVSGKSGVGYVTKFDTKDH
                     DVKIAAEVKDFDPTEYMGKKDAKRMDLFSQYAIAATKQALEDSGIDLEKVDLDRAGTI
                     IG"
     gene            complement(331..561)
                     /gene="acpP"
                     /locus_tag="VFD79_08880"
     CDS             complement(331..561)
                     /gene="acpP"
                     /locus_tag="VFD79_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004825905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZH94204.1"
                     /translation="MVFDKVKEIIAEQLDVEVERVTMETSLMKDLEADSLDAVEIMMA
                     LEDEFEIEVPDDDAEKFTNIGDIVKYVEAKLA"
     gene            complement(581..1321)
                     /gene="fabG"
                     /locus_tag="VFD79_08885"
     CDS             complement(581..1321)
                     /gene="fabG"
                     /locus_tag="VFD79_08885"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HZH94205.1"
                     /translation="MMNGKVALVTGGSRGIGRNIAGKLAKEGFHVAITYNSNEDLAME
                     VVKDLEESGVKAACYKANISIKDQVDSLFKEIENDLGSVDVLVNNAGITKDNLLIRMS
                     EDDWDSVMDVNLKGAFLTTKAVARGMMKKRYGKIINISSVVGVMGNPGQGNYSASKAG
                     LIGFTKSMARELAGRGIRVNAIAPGFIRTDMTDSLKDEFKEAMLSNIPLGYFAEPDEV
                     SNLVAFLASEKSDYITGQVIQIDGGMNI"
     gene            complement(1323..2276)
                     /gene="fabD"
                     /locus_tag="VFD79_08890"
     CDS             complement(1323..2276)
                     /gene="fabD"
                     /locus_tag="VFD79_08890"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HZH94206.1"
                     /translation="MSKRAFIFPGQGAQHVGMGQDFFQQIKESKMVFEEADDTLKSPL
                     SKVIFEGEEEDLKKTETTQPAILTASIAMLKALQKEGIDCDYTAGLSLGEYSSLVLSK
                     ALRFQDALSIVRQRGKFMQEAVPNGVGGMAAILGLDRSRLPEVLLAAKDYGIVEVANY
                     NSPEQIVISGEKKGLRIACAKALEEGAKKAVELPVSAPFHTSMLSPAGERLKLELEKV
                     CIGDFQKKVITNVDGKAINSKDQLVSKLVDQVSNSVLWHQSIEYMIDEGVDTFIEIGP
                     GKSLSGFVKRIGKSMGKDVKSMNISDMDSFRSTVEKLTKGA"
     gene            complement(2269..3228)
                     /gene="fabK"
                     /locus_tag="VFD79_08895"
     CDS             complement(2269..3228)
                     /gene="fabK"
                     /locus_tag="VFD79_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HZH94207.1"
                     /translation="MFDSRVKDLLGIKYPIFQGGMAWVATSELAAAVSDAGGLGIIAA
                     GNAPGDYVREEIRKLRKMTDKPFGVNIMLLSPFVEDIVDIVCEEGVPVVTTGAGNPGK
                     FLERFREHNIKVIPVVPTVSLAKRLEKQGVDALIVEGTEAGGHIGELTTMALVPQVRD
                     AVGIPIIAAGGIADGRGIVAALALGADGVQLGTRFVCSTEAKVHQNYKDKLINAKDRD
                     ALVTGRSTGHPVRVIKNQFTREFMELEKSGASVEDLEKMGSGRLRLAVVEGDVDKGSV
                     MSGQIAGMIKDIKPCNEIIQQLIQEAQQVLGKLKNQEGGETHE"
     gene            complement(3221..4228)
                     /locus_tag="VFD79_08900"
     CDS             complement(3221..4228)
                     /locus_tag="VFD79_08900"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HZH94208.1"
                     /translation="MNKISVGITGIGSYVPDNVMTNDDLSKIVETSHEWIVSRTGIEE
                     RRIADKDQASSDLATMAAWKALEDAAMGPEDLDLIIVATATPDYMFPSTAAMVQKNIG
                     AVKAAAFDMSVGCSGFVYGLVTGANFISTGMYKKVMVIGAEILSKVVDWEDRNTCVLF
                     GDGAGACILEPCDESFGILSSELGSDGVKGDVLIIPGGGSKNPATEETVKNKLHAIRM
                     DGKEVFKFAVRVMEQSSLRVLESAGLAKEDLDFMISHQANSRIIDAAMKKLELNKDKT
                     HVNLTKYGNTSAASVPVALDEAVKGELIKKGDNLLLIAFGAGLSWASVVLKWNKEVRN
                     V"
     gene            4393..4824
                     /gene="fabZ"
                     /locus_tag="VFD79_08905"
     CDS             4393..4824
                     /gene="fabZ"
                     /locus_tag="VFD79_08905"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HZH94209.1"
                     /translation="MKSYDIKDIMELIPHRQPFLFVDKIHVTEEGKSGTGYKMVTINE
                     PFFQGHFPGNPVMPGVIIIETMAQVGAVILLARDDFKGKTPYFAGINKFRFKRKVRPG
                     DILKMEVEITKLRGSIGIGQGVAYVDGEVAAEGEFLFAIDK"
     gene            4840..5898
                     /locus_tag="VFD79_08910"
     CDS             4840..5898
                     /locus_tag="VFD79_08910"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HZH94210.1"
                     /translation="MKQLIIGDKSTRLPVIQGGMGVGVSLSGLASAVANRGGIGVISG
                     VQIGFRETDFMTNTMEANIRALRKEIRLAREKSPLGILGVNLMVAIENYAETVKAAVE
                     EKIDLIISGAGLPNDLPGLVKGSKTKIAPIVSSGKAAAVITKLWMKRHDYFPELIIIE
                     GPLAGGHLGFSKEVLASGEIPRLEDIFTEVKKALKPFEEKAGKTAQIVVAGGIYNGED
                     IKRFLDFGADGVQMGTRFVATEECDAHEDFKIAYVNSNEGTIDYIVSPVGMPGQAVMT
                     PFIERVSLEREKITKCYNCLSACNPATTPYCITSALIRAVQGDVDNGLVFIGKRGHLV
                     DRISTVDALMDEFEAALI"
     gene            complement(5934..7079)
                     /locus_tag="VFD79_08915"
     CDS             complement(5934..7079)
                     /locus_tag="VFD79_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZH94211.1"
                     /translation="MKRVYLDNGATSFPKAPGVAEAMMDYIVNNGANVGRGIYETSFH
                     AENIVFDTREKLAKLFNFKDPANVVFTKNITESLNIVLKGLLREDDHVIVSSMEHNAV
                     MRPLNSLGKRGVTFSRVQCNQVGELNPEDILGQIKSSTRAIVMTHASNVCGTILDLEE
                     VGKICRENGLIFIVDAAQTAGFMDVDYNGLNADVIAFTGHKSLLGPQGMGGFAIGEDL
                     ARDIDTFMEGGTGSLSDKEIQPEYMPDKLEAGTPNIPGIFGLNAALNYLEERGIENLR
                     KVEMNLVQEFLDGLKKIEGIEIIGKKSITGRTGVVSTDFPGRDNADISFRLNEEYGIA
                     TRCGMHCAPSAHETLKTFPRGTVRFSFSHFNTREEVEYTLEAIKKLV"
     gene            7263..8327
                     /gene="yedE"
                     /locus_tag="VFD79_08920"
     CDS             7263..8327
                     /gene="yedE"
                     /locus_tag="VFD79_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HZH94212.1"
                     /translation="MKEKRSIIFAGAVVGIISVLLVYFGNPANMGFCIACFLRDIAGG
                     IGLHRAAVVQYIRPEVIGLVLGAFLVSIKNKEFKTNGGSSPFTRFVLGALVMIGALMF
                     LGCPLRMVLRLAGGDLNALLGIGGYTVGIYFGVLFLNKGFSLKRSYKMPSIEGYIFPG
                     INIGLLVLLISGAGFLFFSSEGPGSMHAPIWISLAAGLIVGWLAQRTRLCMVGGIRDM
                     ILFRDSHLISGFVAILVFALAGNVFLGYFNLGFADQPIAHSDGLWNFMGMLLAGWGSV
                     LLGGCPMRQLILAGEGNTDSAVSVMGMVAGAAIAHNFGLASSGAGPTANGQIAVILSI
                     VLVAIISASNSELVTKRAEA"
     gene            8341..8547
                     /locus_tag="VFD79_08925"
     CDS             8341..8547
                     /locus_tag="VFD79_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HZH94213.1"
                     /translation="MNIIDARGMSCPQPVLMTKNALKGNPGIVQIIVDNETAKGNIER
                     YLNHEGFSVEFQNQDDDILIKGSR"
     gene            8549..8788
                     /locus_tag="VFD79_08930"
     CDS             8549..8788
                     /locus_tag="VFD79_08930"
                     /inference="COORDINATES: protein motif:HMM:NF023251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HZH94214.1"
                     /translation="MKSYMILFFTHSGAIKFQRKLERLNVSCKLMPVPRSLSSNCGIG
                     ARVSHDGNILILADNEVEKVYEIQEDDYREIFHQS"
     gene            complement(8828..9385)
                     /locus_tag="VFD79_08935"
     CDS             complement(8828..9385)
                     /locus_tag="VFD79_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HZH94215.1"
                     /translation="MEEKLIIAGFGGQGVMAMGQLLTYSGMVEGRNVSWLPSYGPEMR
                     GGTANCNVTISSEPVGSPIVVEATTAVVLNLPSLEKFEDSVVPGGNLMINSSLIAKKS
                     KRDDIDVYYIPANEIANEIGNTRIANMVMLGAYIEITGAIKIESVYEAFGKVFGETKA
                     HLLPINKDALMRGAEIAREQMKVKA"
     gene            complement(9388..10134)
                     /locus_tag="VFD79_08940"
     CDS             complement(9388..10134)
                     /locus_tag="VFD79_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZH94216.1"
                     /translation="MAIIFEKTKGLTDKPTHYCPGCTHGIIHRLVAEVMEEMDLLDEA
                     IGVAPVGCSVLAYDYFNVDMHEAAHGRAPAVATGIKRVHPNNFVFTYQGDGDLASIGT
                     AEVVHAAHRGEKFTTIFVNNAIYGMTGGQMAPTTLIGQKTTTSPYGRDEATTGKPIKI
                     SEMLATIDGAKFVERVAVNTPANIRKAKKAIREAFKVQIEGKGFSIVEVLSTCPTNWG
                     LTPVESMKWLEKDMIPYYPLGNFRKPEEVE"
     gene            complement(10134..>10985)
                     /gene="vorB"
                     /locus_tag="VFD79_08945"
     CDS             complement(10134..>10985)
                     /gene="vorB"
                     /locus_tag="VFD79_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019108374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HZH94217.1"
                     /translation="ARVMTSSSSPGIALKQEGISYCAGAELPCVIVDMMRGGPGLGTI
                     QPAQGDYFLATRGGGNGDYRLIVLAPADVQELVDLVMEGFDLADLYRTPVMILGDGMI
                     GQMMEPVEFNYKPRADLPEKDWATKGKGKGKQRNVINSLYLQAEELEKHNHKLQAKYA
                     KIRENEKKVEEYNVEGADIVITAYGTTSRIAKTAIAKLEKEGYKVGMIRPISLYPFPN
                     EAFERIGDDCKGVLTVEMSTGQMIDDVKISLQGRIPTYFHGRSGGMVPEPEEIVEKVK
                     EILGGDK"
BASE COUNT         2803 a         2657 c         2162 g         3363 t
ORIGIN      
        1 ccaattattg taccggccct gtcaaggtct accttctcaa gatcaatccc tgaatcctca
       61 agtgcctgct ttgttgcagc aatggcatac tggctgaaaa ggtccattct ctttgcatcc
      121 ttcttgccca tatactcagt ggggtcaaaa tcctttactt cagctgcaat cttcacatcg
      181 tggtccttgg tgtcaaactt agttacatat cctactccgg attttccgga aacaattgaa
      241 ttccagaact cctctttacc cgatcccaca ggtgttatgg caccaagtcc ggtgattact
      301 actctattca tccttacacc tctctttagt ctaagctaac ttagcctcca cgtattttac
      361 aatgtcaccg atgttggtga acttttctgc atcatcatca ggcacttcaa tttcgaactc
      421 gtcctcaagt gccatcatga tttcaacagc atcaagtgaa tctgcctcaa gatccttcat
      481 tagtgaagtt tccattgtta ctctttcaac ctcaacatcc aactgctctg caataatttc
      541 ctttacctta tcaaatacca tttttattcc tcctaaaaat ttaaatgttc attccaccgt
      601 ctatctgtat tacctggcca gttatataat ctgatttctc acttgccaga aacgccacca
      661 aattacttac ttcatcaggc tctgcgaaat accccaaggg gatgtttgaa agcattgcct
      721 ccttgaactc atccttaaga ctatctgtca tatctgttct gatgaatcca ggcgctattg
      781 catttaccct tattccacgg cctgccagct cccttgccat tgattttgta aagccaatga
      841 gtcctgcctt ggaggcagaa tagttaccct gtccgggatt acccatgact cctactactg
      901 aggagatgtt gattatcttt ccataccttt tcttcatcat tcctctggcg accgcctttg
      961 ttgtcagaaa agctccctta aggttaacat ccatcacaga atcccaatca tcctcggaca
     1021 ttctgattag aagattatcc tttgtgattc ccgcgttgtt gactaagaca tccaccgacc
     1081 caaggtcatt ctcaatttcc ttgaaaagac tgtcaacctg gtcctttatg ctgatgttgg
     1141 ccttgtagca ggcagccttt acccctgatt cttccaggtc cttcaccact tccattgcca
     1201 ggtcttcgtt actgttatat gttatggcca catgaaagcc ttcttttgca agcttccctg
     1261 caatgtttct tccaattcct ctgctgcctc cagtgaccaa agctactttt ccattcataa
     1321 ttctaagccc cctttgtcag cttttcaaca gtacttctaa agctatccat gtcagaaata
     1381 ttcatggatt tcacatcctt gcccattgac tttccaattc tcttcacaaa gcctgacagg
     1441 gatttcccgg gacctatttc aatgaatgtg tccacacctt catctatcat gtattcaatt
     1501 gattgatgcc ataggacgga atttgaaacc tggtcaacca gttttgacac tagctgatcc
     1561 ttgctgttga ttgccttgcc gtcaacattt gtaataacct tcttctggaa atcacctatg
     1621 catacctttt caagctcaag cttgagtctc tcccctgcag gagaaagcat tgatgtatgg
     1681 aatggagcgc ttacaggtag ctccactgcc ttcttggccc cttcttccag agccttggca
     1741 catgctattc taagtccctt tttctctcct gagatgacaa tttgttcagg gctattgtaa
     1801 ttagcaacct ccacaatccc atagtccttg gctgcaagaa gtacttcagg caaccttgat
     1861 ctatccaaac ctaaaatggc cgccattccc cctactccat tgggaactgc ctcctgcatg
     1921 aactttcccc tctgtctaac aatagaaagg gcgtcctgga atctcaatgc cttgcttaga
     1981 accagtgagc tgtattcgcc aagactgagc cctgctgtgt agtcacagtc aattccctcc
     2041 ttctgaagtg ccttaagcat ggctatgctg gcagttagaa ttgcaggttg agttgtctcg
     2101 gtctttttca ggtcttcctc ttccccttca aatatcacct tagaaagtgg gcttttcaga
     2161 gtgtcgtcag cctcttcaaa taccatcttt gactctttta tctgctgaaa aaagtcctgt
     2221 cccataccaa catgctgggc tccctgtcca ggaaagataa atgctcgttt actcatgtgt
     2281 ctcacctccc tcctggtttt tcagcttgcc caaaacctgc tgggcttcct gaatcaattg
     2341 ttgaattatt tcattacatg gctttatatc cttgatcatg ccggcgattt gtccagacat
     2401 tacagatccc ttgtcaacat caccttccac aaccgccaaa cgaagcctgc ctgatcccat
     2461 tttttcaaga tcttcaactg atgctccaga cttctcaagc tccatgaact cccttgtaaa
     2521 ttggttctta attaccctaa caggatgacc tgtgcttctg ccggttacca gcgcatccct
     2581 gtcctttgca tttattagct tgtccttgta attctggtgt accttggctt cagttgaaca
     2641 aacaaatctc gtcccaagct gaacgccgtc cgctcccagt gccaaggctg cgactatacc
     2701 ccttccatcg gcgatgcctc cggcagcgat tatcggtatc cccactgcat cacggacctg
     2761 gggcacaaga gccatggttg ttagctcacc aatgtgtcca cctgcctcgg ttccttctac
     2821 aatcaaagca tccacgccct gcttttccaa tctcttggcc aaggatactg taggtaccac
     2881 aggtatgacc ttgatattat gttccctgaa tctttcaaga aactttcctg gattccccgc
     2941 acctgtggtt acaacaggga ctccttcctc acatacaata tctacaatat cctcgacaaa
     3001 tggtgacaac agcattatgt taacaccaaa tggcttgtca gtcattttcc ttagcttcct
     3061 gatctcttcc cgaacatagt ccccaggtgc gttgcccgct gcgattattc caagtccccc
     3121 agcatctgaa actgcagctg ccagttcact tgttgcaacc caggccatcc ctccttggaa
     3181 tatggggtac ttgattccaa gaagatcctt tactcttgaa tcaaacattt cttacctcct
     3241 tgttccattt aaggactaca gatgcccatg acaatcctgc tccaaatgct atcaaaagca
     3301 aattgtcgcc tttttttata agctcacctt taacagcctc atccagagcg actggcacgg
     3361 aggctgcaga agtgttccca tattttgtaa ggttaacatg ggtcttatcc ttattcaatt
     3421 ccagcttctt catggctgca tcgatgattc tgctgtttgc ctggtgggag atcataaagt
     3481 ccagatcctc tttagccagt cctgcgctct ccagaaccct aagtgaggat tgctccatta
     3541 ctctcacggc aaacttaaac acttccttac catccatcct gatggcatga agcttgttct
     3601 ttaccgtctc ctctgttgca gggtttttag accctcctcc aggtattatt aaaacgtctc
     3661 ctttgacacc atctgaccca agctctgaag aaagaatccc aaaactttcg tcacatggtt
     3721 ccaaaatgca cgcccctgct ccgtctccaa acaaaacgca ggtatttctg tcctcccaat
     3781 ccactacctt ggataggatt tctgctccaa tcaccattac tttcttgtac attcccgtgg
     3841 atatgaagtt agctccggtt acaagtccat atacaaatcc tgaacatccg acgctcatat
     3901 caaaggctgc tgcctttact gctccaatat tcttctgaac cattgcagcc gttgaaggaa
     3961 acatatagtc cggtgtggca gttgctacga ttatcaggtc caggtcttca ggccccattg
     4021 ctgcatcctc caaagccttc catgccgcca tggtcgcaag atctgagctt gcctgatcct
     4081 tgtccgcaat tcgtcgctcc tcaatgccgg ttcttgagac tatccattca tgactggttt
     4141 caacaatctt tgataggtca tcgttggtca tgacattatc gggaacatag ctccctatcc
     4201 cggtgattcc tacggatatc ttattcaatt taccacctcc tgttattagg gggattaaaa
     4261 gtttgcaaat ttttcgtttt taaattatta ttgattatag gtacatgcaa actcaagaac
     4321 agtatagtac aaaatgagaa tttaatcaat attttatttt ctcatactta gacctaaagg
     4381 aggtgtaaag aagtgaaatc ctatgatatc aaggatatta tggaactgat tccccacagg
     4441 caaccctttc tatttgtgga taagatccat gtcacagagg aaggaaagtc tggaaccggc
     4501 tacaagatgg tcactatcaa tgaacccttt tttcagggtc attttccagg gaatcctgta
     4561 atgcccggag ttatcatcat tgagacaatg gcacaggtgg gggcagtaat cctcttggcc
     4621 agagacgact ttaaaggaaa aactccatat tttgcaggta ttaacaagtt caggttcaaa
     4681 aggaaggtaa ggcctggaga catcctgaag atggaggttg aaattacaaa gcttaggggt
     4741 tcaataggaa tcggccaggg tgtagcctat gttgatggcg aggttgcagc agagggcgaa
     4801 ttcctgtttg ccatagataa atagagagaa ggtgtgttga tgaaacaatt gataattgga
     4861 gataagagta cacgtctacc agtgatccaa ggtggaatgg gtgtgggagt atctctttca
     4921 gggcttgcct cagctgttgc caacaggggt ggaataggtg tcatatccgg agtccagatt
     4981 ggctttaggg aaacagactt tatgaccaac accatggaag ccaacataag ggccttaagg
     5041 aaggaaatta gacttgcaag ggagaagagc ccacttggca tacttggggt aaatcttatg
     5101 gttgccatag aaaattacgc tgaaaccgtc aaggctgcag tggaagaaaa aattgatttg
     5161 ataatttctg gagcaggttt gccaaacgac ctacctggac ttgtaaaggg aagcaagaca
     5221 aaaattgctc ccatagtctc ctcaggtaag gctgctgcag taattaccaa gttatggatg
     5281 aagcgtcatg actatttccc ggagcttata ataatcgagg gacccctggc aggaggccat
     5341 ctcggcttta gcaaggaggt tcttgccagc ggtgagattc caaggcttga ggatattttt
     5401 accgaggtta aaaaagccct taaacccttc gaggaaaaag ccggaaaaac tgcacaaatt
     5461 gtggttgcag gtggaattta caatggtgag gacataaaga gattccttga ttttggagca
     5521 gatggagttc agatgggcac aagattcgtt gctactgagg aatgtgacgc acatgaggat
     5581 ttcaagattg cctatgtaaa ttccaatgaa gggacaatag attatatagt gagtcctgtt
     5641 gggatgcctg gacaggctgt tatgacacca tttattgaga gggtgtcctt ggaaagggaa
     5701 aagatcacaa agtgctacaa ctgtctaagt gcctgcaatc cagccactac accatattgc
     5761 ataacaagtg ctcttataag ggccgttcaa ggggatgtgg acaatggatt ggtgttcatt
     5821 gggaaaagag gccatcttgt tgacagaata tcaacagtgg atgccctaat ggatgagttt
     5881 gaagcagcct tgatatgatt tttaaagcca gaccttgcgg tctggcttta tctttagact
     5941 agtttcttta ttgcctccag cgtatattca acctcttccc ttgtattgaa gtgtgaaaaa
     6001 ctgaatctga cagttcccct tggaaatgtc ttaagagtct catgggctga tggagcgcaa
     6061 tgcatcccac atctggttgc tattccatac tcctcattga gcctgaagga tatgtccgca
     6121 ttgtcccttc caggaaaatc tgttgaaaca actccagtac ggcctgttat ggactttttc
     6181 ccaattattt ctattccttc aatctttttt aatccatcca ggaattcctg gaccagattc
     6241 atctcgacct ttctgagatt ttcaattcct cgctcttcca gataattcaa tgctgcatta
     6301 agtccaaata tccctgggat atttggggtt ccagcctcaa gcttatcagg catatattcg
     6361 ggctggattt ccttgtcaga tagactccct gttccgcctt ccatgaaggt atcaatatcc
     6421 cttgccaaat cctcgcctat tgcaaatcct cccatcccct ggggtccaag gaggctcttg
     6481 tgaccggtaa aggctatcac atccgcattt aggccattat aatccacatc catgaatccg
     6541 gctgtctgtg cagcatccac aataaatatc aagccatttt ccctgcatat cttacccact
     6601 tcctcaaggt ccagtatggt tccacaaaca tttgaagcat gggtcatgac aattgcgcgt
     6661 gtgctgcttt ttatttgtcc caagatatcc tcaggattca actcacctac ctggttgcac
     6721 tgtacccttg aaaaagttac acctcttttt cctagagaat tcaatggtct cataacagca
     6781 ttgtgctcca ttgatgaaac gattacatgg tcgtcttccc ttaaaagtcc cttaagtaca
     6841 atattcaggc tttcagttat attttttgta aaaactacgt tggctggatc cttgaagttg
     6901 aagagttttg ccagcttctc tctggtatca aaaacaatat tctctgcatg gaaggaggtc
     6961 tcatatattc ccctgccaac atttgcaccg ttattaacta tgtaatccat catggcttct
     7021 gcaacacctg gagcctttgg aaaactagtt gctccattat cgagatatac ccttttcata
     7081 aacaccatcc tcccctgatt cctaatttga tgctactcct ttttgatagt aattacaatc
     7141 ggtattaaat aatctaatag ataggcattt tcaattagta aatccaattt aacatgcatc
     7201 atgagaattg ttataatcat aacgaatata tgtcattatt acataggaat aggaggattg
     7261 aaatgaaaga aaaaagaagt attatctttg ccggggcggt agtgggaata atctccgtac
     7321 tgcttgtcta tttcggaaac cctgccaata tgggtttttg cattgcctgt tttcttcggg
     7381 acattgcagg tggaataggc ttacaccgtg cagcagttgt tcagtacata aggcctgagg
     7441 ttataggcct ggtcctgggt gcatttttag tgtccatcaa gaacaaggag tttaaaacaa
     7501 acggagggtc ttcacccttc actagatttg ttcttggggc tttggttatg ataggtgcct
     7561 tgatgtttct aggatgtccc ctgagaatgg tcctaaggct ggcaggagga gaccttaacg
     7621 cacttcttgg aatcggaggc tatactgtag gaatatactt tggtgtattg ttcctgaaca
     7681 aggggttcag cttgaagagg agttacaaaa tgccttccat cgaaggatac atattcccag
     7741 gaataaacat tggcctgctg gttctattga tttccggtgc cggtttcctg tttttcagca
     7801 gcgaaggacc tggttccatg catgccccca tctggatatc acttgcagct ggtctaattg
     7861 ttgggtggct tgcccaaagg accagacttt gtatggtagg tggaatcagg gatatgatcc
     7921 tttttaggga ttcccatcta atttcaggct ttgtggcaat ccttgttttt gccctagcag
     7981 gcaatgtatt tctgggatac tttaatctgg gttttgctga tcagccaata gcccatagtg
     8041 atggactgtg gaatttcatg ggcatgcttc ttgctggatg gggttccgtc ctactaggtg
     8101 gatgtccaat gagacagctt atcctagcag gagaagggaa cactgattca gccgtgagtg
     8161 ttatgggaat ggtagcgggg gctgctattg cacataattt tggacttgct tcaagcggtg
     8221 ccggacctac agccaatgga caaattgctg taatccttag cattgttcta gtcgcaataa
     8281 tctcagcttc caattccgaa cttgtcacaa aacgtgcgga agcttaaagg aggaaaatac
     8341 atgaatataa ttgatgcaag aggaatgtcc tgtccacagc ctgtgcttat gacaaaaaat
     8401 gcacttaaag gcaatcctgg cattgtccag atcattgtgg acaacgagac tgccaagggg
     8461 aatatagaaa gatatttgaa tcacgaaggc ttttctgtgg agtttcagaa tcaggatgat
     8521 gatattctga taaagggaag caggtaggat gaaaagctac atgatccttt tctttaccca
     8581 ttcaggagca ataaagtttc agagaaagct tgaaaggttg aatgtttcct gcaagttgat
     8641 gcctgtaccc agatcattga gctccaactg tggaataggg gcaagagtca gtcatgatgg
     8701 taatatactt atacttgctg acaatgaggt tgaaaaggtg tacgaaatac aagaggatga
     8761 ttatagggaa atatttcatc aaagctaaca aaagccagac cttgcggtct ggctttatct
     8821 attgctacta tgcctttact ttcatttgtt ctctagctat ctctgcaccc ctcattaggg
     8881 catccttgtt tattggaagg aggtgagcct tggtttctcc aaataccttc ccaaaggcct
     8941 catacaccga ttcaattttg attgctccag taatctctat atatgcacca agcataacca
     9001 tgtttgctat tctggtattt cctatttcat ttgcaatttc gtttgctggt atatagtata
     9061 cgtctatatc atctctttta gactttttag ctataagaga tgaattgatc ataaggtttc
     9121 cacctggaac cacactatcc tcgaacttct caagggatgg aaggttaagt actacagctg
     9181 ttgtcgcctc cacaactatt ggagatccaa ctggctctga cgatattgta acgttgcagt
     9241 ttgcagtacc tccacgcatt tctggaccgt atgacggcaa ccatgataca ttcctaccct
     9301 ctaccattcc agaatatgtc agtagctgtc ccatggccat tacaccctgt ccaccaaatc
     9361 ctgcgattat taatttttct tccatttcta ttctacctcc tctggctttc tgaagttacc
     9421 caatggatag taaggaatca tatccttttc caaccatttc attgattcaa caggtgtaag
     9481 accccagttt gtcggacatg ttgacaggac ctcaacaata ctgaatccct taccctctat
     9541 ctgaaccttg aatgcctccc ttatggcttt cttggctttt ctgatgtttg caggagtgtt
     9601 gactgcaacc ctttcaacaa attttgcacc atcgattgta gccagcatct ctgatatctt
     9661 gattggttta cctgttgtgg cttcatccct accatatgga gaagttgtcg tcttttgtcc
     9721 aataagggtg gttggtgcca tttgtccacc agtcatgccg tatattgcat tgtttacgaa
     9781 aattgttgta aacttctcac ccctgtgggc tgcatgaaca acttctgcag taccgattga
     9841 agccaggtca ccatctccct gataggtgaa tacgaaatta tttgggtgaa cccttttaat
     9901 accagtggct acagcaggag ctctaccatg agccgcctca tgcatatcta cgttgaaata
     9961 gtcatatgcc aatactgaac aacctacagg tgcaacacct atggcttcat ccagaagatc
    10021 catttcctcc attacctcgg caacaagtct gtgaattatt ccgtgtgtac agcctggaca
    10081 ataatgggta ggtttgtcag ttaatccttt tgttttctca aatataattg ccattatttg
    10141 tcacctccaa gaatttcctt aaccttttca acaatttcct caggctcagg aaccattcca
    10201 cctgaacgac catggaagta tgttggaatt cttccctgca gtgaaatctt aacatcatct
    10261 atcatctgtc ctgtactcat ctcaacagta agtactcctt tacaatcgtc tccaattctc
    10321 tcgaatgctt catttgggaa tggatagagg cttattggtc taatcatgcc aaccttgtag
    10381 ccttccttct caagctttgc tatggcagtt ttggcgatac gggatgttgt accgtaagct
    10441 gtaattacta tatccgcacc ctcaacatta tattcttcta ccttcttctc attctccctg
    10501 atttttgcat attttgcctg aagcttgtgg ttgtgcttct caagctcctc tgcctgaagg
    10561 taaagtgagt tgattacgtt tctctgcttt ccctttccct ttccttttgt tgcccagtcc
    10621 ttttcaggaa ggtcagctct tggcttatag ttaaactcca caggctccat catctggcca
    10681 atcattccat cgccaaggat cattacaggt gttctgtaaa ggtctgcaag gtcaaagccc
    10741 tccattacaa ggtccaccaa ttcctgaaca tctgccggtg ccaatactat cagtctgtag
    10801 tctccatttc ctccgcctct ggttgcaagg aagtaatctc cctgagctgg ttggattgta
    10861 ccaagtcctg gtccacccct catcatatca acgattacgc atggaagctc cgctcccgca
    10921 caatatgaaa taccttcctg cttcagtgca attccagggc ttgatgatga tgtcataact
    10981 cttgc
//
LOCUS       DASRQF010000332         3799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Tissierellaceae bacterium isolate SMAG_U10315
            k141_317192, whole genome shotgun sequence.
ACCESSION   DASRQF010000332 DASRQF010000000
VERSION     DASRQF010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963839
            Sequence Read Archive: SRR5690554
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tissierellaceae bacterium (soil metagenome)
  ORGANISM  Tissierellaceae bacterium
            Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:45:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,796
            CDSs (total)                      :: 1,777
            Genes (coding)                    :: 1,768
            CDSs (with protein)               :: 1,768
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 11
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 4 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Tissierellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317192"
                     /isolate="SMAG_U10315"
                     /isolation_source="soil"
                     /db_xref="taxon:2562714"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="VFD79_08950"
     CDS             complement(<1..695)
                     /locus_tag="VFD79_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HZH94218.1"
                     /translation="MERNLDQIWEDVLNLIKVELTEVSFNTWLKSIEPISLTDEEIVL
                     GAPNDFTKGILEGRYFNLIRNAINQISGSNYNIKFIIPGEEVQPSPPSPKEVENEDIL
                     NKSKLNPKYSFSTFVTGKSNEFAHAASVAVAEAPAQAYNPLFIYGGVGLGKTHLMHAI
                     GHYILSQNPNSKVVYVSSEKFTNELINSIREYRNEEFRNKYRNVDVLLIDDIQFIAGK
                     EGTQEEFFHTFNAL"
     gene            1213..1347
                     /gene="rpmH"
                     /locus_tag="VFD79_08955"
     CDS             1213..1347
                     /gene="rpmH"
                     /locus_tag="VFD79_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HZH94219.1"
                     /translation="MKRTYQPKKRQRSKEHGFRKRMKTKSGRSILKARRKRGRKRLSA
                     "
     gene            1429..1764
                     /gene="rnpA"
                     /locus_tag="VFD79_08960"
     CDS             1429..1764
                     /gene="rnpA"
                     /locus_tag="VFD79_08960"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HZH94220.1"
                     /translation="MEKKNRLRKNMEFKKVYKARKNFWNRNLILYIKRNGTKETRIGV
                     SITRKVGNAVVRNKLKRRIKELNRRYIESLKEGYDIVIIPKKNAVDLSFKDLESALKH
                     IYKLSGVWK"
     gene            1774..1989
                     /gene="yidD"
                     /locus_tag="VFD79_08965"
     CDS             1774..1989
                     /gene="yidD"
                     /locus_tag="VFD79_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002839864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HZH94221.1"
                     /translation="MTRIAILLIRFYQRVISRYILTGNNCRFYPTCSEYSLQAYKKYG
                     FIKGSYLSAKRILRCNPFNEGGYDPLK"
     gene            2004..2717
                     /locus_tag="VFD79_08970"
     CDS             2004..2717
                     /locus_tag="VFD79_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HZH94222.1"
                     /translation="MIGFLGNILGSLLRFVYDIVSSTLGSEPESISYYALAIILTTIV
                     FKLMLLPINLQQTRSTRKMSEVQPLMKEIQTKYKKDPQKMQEKMQELYKEHKYNPASG
                     CLILLVQLPIIFAFFTVFREPQTYAFSDPGMYQSMAKNFLWIANLENPDPLLWGLPLL
                     AAATTFLQSKTMSTSSSTDPQAQSTQKIMNYMLPVMIFWASRSFPAGLALYWVVSNFF
                     TIVQQVITNRSLMKIKGAK"
     gene            2719..3345
                     /gene="jag"
                     /locus_tag="VFD79_08975"
     CDS             2719..3345
                     /gene="jag"
                     /locus_tag="VFD79_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HZH94223.1"
                     /translation="MKFVVKTAKTLEEALQEALTELKATEKDVEYEVIEEASKGLFGL
                     IGAKEAKIKVVLKNDPEETADKFLDGVLKGMGIIALHDISIEDDIMKIEINNISSSDM
                     GILIGKRGNTLDSIQYLLSLAINKDRENYLKVLLDAEGYRAKREETLKRLANKMAEKA
                     KYSKRPVKLEPMNPYERRIIHSALQEFDGVSTYSEGNEPYRRVVIVSK"
     gene            3522..>3799
                     /locus_tag="VFD79_08980"
     CDS             3522..>3799
                     /locus_tag="VFD79_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HZH94224.1"
                     /translation="MSETIAAISTAVGEAGIGIVRMSGRESLEIAKRVFARSNDQEIQ
                     EKDNKRLLYGHILEDESIIDEVLVAFMHEPYTYTRENMVEIYCHGGIIP"
BASE COUNT         1281 a          660 c          784 g         1074 t
ORIGIN      
        1 agtgcattga aggtatggaa gaattcctcc tgggtacctt cctttcctgc aataaactga
       61 atatcatcta ttagaagaac atctacattt ctatatttat tcctgaactc ctcgttccta
      121 tattccctga tagaattgat aagctcattt gtgaactttt cgcttgatac ataaaccacc
      181 ttggagtttg gattctggct gagaatataa tgtccaatgg catgcatcag gtgagttttt
      241 ccaagcccta ccccaccgta tatgaatagg gggttatatg cttgagctgg tgcctctgca
      301 acggcaactg atgccgcatg ggcaaattcg ttgcttttac ccgtaacaaa ggtactgaag
      361 gaatattttg gatttagctt ggatttgttt agaatatcct cattttcaac ttcctttggc
      421 gatgggggtg atggctgcac ttcctccccg ggtataataa atttaatgtt ataattactt
      481 ccgcttatct ggttgattgc atttcttatg agattgaagt acctcccctc cagtattcct
      541 ttggtgaaat cattgggggc tccaagcaca atctcttcgt ctgtcaggct tatgggttca
      601 attgatttta gccatgtatt gaagctgacc tctgtcaatt caaccttgat aagattgagg
      661 acatcctccc agatctggtc caaattcctt tccatataat aacctcccta aaaattatca
      721 acaaatttat caacagaaaa ccattgacta ttcatataaa gatgtataat ggtttctgtg
      781 gataaaaata tcaactcatt gttgattaag taaaaaaaag cttaagtata tttcttgtcc
      841 acactatcca taggggcata ttgtggataa aaacttaaac aaattacgat tctgtaatat
      901 aaaaacagtg attatttaaa agttatacac aatctatcca caaaatgtga ataacttcga
      961 cttatccact gctattaaaa acttatcaac atcctctact atcttatcaa ataaacggtg
     1021 gatattcaat aaaattatcc acagagccaa tatggtgtgg aaatcgtgtt ttgtggatat
     1081 tagtcaatta aaatccttga cacacaaggg cttaatacat ataatttata gtagtgtttt
     1141 gtgatttcaa ttagtaaact ctatattgta tatataggta ataactttta ttacttgaag
     1201 gaggtgtgtt gggtgaagag aacgtatcaa ccaaaaaaga gacaaagaag caaggaacac
     1261 ggttttagaa aaagaatgaa aaccaaatct ggcagaagca tacttaaagc tagaagaaaa
     1321 agaggtagaa aaagactttc tgcataaggc cgcttgttag tggccttttc taacgaaagg
     1381 gaaagatttt tcttctggca ggtaaaggcc atttaaggtg gaattataat ggaaaagaaa
     1441 aacagactta ggaaaaatat ggagtttaaa aaggtttata aagcacgaaa gaatttctgg
     1501 aacagaaatc tgattcttta tatcaaaagg aatggaacaa aagagaccag gattggagta
     1561 tcaatcacca gaaaagttgg aaatgccgtt gtcagaaaca agcttaaaag acgaatcaag
     1621 gaacttaaca gaagatatat tgaaagctta aaggaagggt atgatattgt tatcatacct
     1681 aaaaaaaacg ctgtcgacct atcctttaag gatttggaaa gtgccttgaa gcacatctat
     1741 aagctatcgg gtgtttggaa ataaggtgat taaatgacac gtatagcgat attgttgata
     1801 agattctacc aaagagttat ctcaagatat attcttactg ggaacaactg cagattttat
     1861 cccacttgct ctgaatattc actacaggca tataagaagt atggatttat aaaaggttca
     1921 tacctgtcag ccaagagaat cttaagatgc aatcccttca atgaaggagg atatgatccg
     1981 cttaaataat gggaggttaa caaatgatag gatttctagg taacatactg ggatcattat
     2041 tgagatttgt atatgatatt gtctcatcta ccttgggttc tgagcccgag tcaatttcat
     2101 actatgcatt ggctattatc ttgactacca tagtattcaa gctaatgctt ttgccgataa
     2161 atctacagca gacaaggtcc acacgtaaaa tgagtgaagt gcagcccttg atgaaggaaa
     2221 tacagaccaa gtacaagaaa gatcctcaga agatgcagga gaagatgcag gagctttaca
     2281 aggagcacaa gtacaatcca gcttcaggct gcctgatact gcttgttcag cttccaataa
     2341 tatttgcctt ctttacggta tttagagagc cacaaaccta cgctttcagt gatcccggaa
     2401 tgtatcaaag catggcgaag aactttctat ggatagcaaa cctggaaaat ccggatccat
     2461 tactatgggg actgccacta ttggctgcag ccacaacatt cctgcagagt aagaccatga
     2521 gcacgtcttc atcaacggac ccacaggctc aatcaactca gaagataatg aattacatgc
     2581 taccagtaat gattttttgg gcatcaagaa gctttccagc cggcctggca ctttattggg
     2641 tagtaagtaa ctttttcaca atagtacagc aagttattac aaaccggtct ttaatgaaaa
     2701 ttaagggggc aaaatagtat gaaatttgtt gtaaaaactg ccaaaactct agaggaagcc
     2761 ctccaagagg ctttgactga gctaaaagct actgagaagg atgttgaata cgaggttatt
     2821 gaggaagcgt caaagggatt gtttggactt attggtgcaa aggaagccaa gataaaggtt
     2881 gtacttaaga atgatccaga ggagacagct gacaagtttc ttgatggagt attgaaggga
     2941 atgggaataa tagcccttca cgacatctcc attgaggatg atatcatgaa gattgagata
     3001 aacaacatat catcatcaga tatgggaata ctcattggaa aacgaggaaa cactctggat
     3061 tccatccaat acctgctgag tctggcaatt aacaaggacc gagagaacta tctcaaggta
     3121 cttcttgatg cagaaggcta cagggcaaag cgtgaggaaa cattgaagag acttgccaac
     3181 aagatggctg aaaaggccaa gtacagcaag agaccagtta agctggaacc aatgaatcca
     3241 tacgagagaa ggatcattca ctcagcattg caggaatttg atggcgttag tacctacagt
     3301 gagggaaatg aaccctatag aagagtcgta atagtatcca aataaagcct ataattacat
     3361 tttaaaaagc ccacaggttg ggcttttgtt ttttgcccac tcccctaccc tatccaggga
     3421 tatgtgaatg ttttttgtaa aaattcaaaa agtgtattga attattgtta taatggaata
     3481 gaatagttaa gataatagta atagttggag gagttgattt tatgtcagag acaattgctg
     3541 ccatctcaac agccgtaggc gaagcgggca ttggaattgt cagaatgagc ggacgggagt
     3601 cacttgaaat tgcaaagcgg gtttttgcaa gatccaatga ccaggagata caggaaaagg
     3661 ataataaaag acttttgtat ggacacatac ttgaggacga gtcaattata gatgaggtgc
     3721 ttgtggcatt tatgcacgag ccatacacct acaccaggga aaatatggtt gaaatatact
     3781 gtcatggagg tattatacc
//
