LOCUS       DASRUK010000001         3846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2342528, whole genome shotgun sequence.
ACCESSION   DASRUK010000001 DASRUK010000000
VERSION     DASRUK010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2342528"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..744
                     /locus_tag="VFF28_00005"
     CDS             79..744
                     /locus_tag="VFF28_00005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02253 family HAD-type hydrolase"
                     /protein_id="HZX44046.1"
                     /translation="MIKAVLFDLDNTLIDFMRMKRNSCEAAISAMIDAGLDINKEAAL
                     KKLFVLYDKHGMEDSSIFQKFLKETTGEIDYRILANAIVSYRAVRASYLQPYPHTDFV
                     LIKLKARGVKLAIVTDAPKLKAWVRLMSMKIGNFFDVVVAFEDTRQLKPSNLPFNAAI
                     GKLDVKPQECLMVGDMPNRDIAGAKKLGMKTCFARYGNDKIRKSNADYTIDDIKDLLK
                     IVK"
     gene            829..978
                     /locus_tag="VFF28_00010"
     CDS             829..978
                     /locus_tag="VFF28_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HZX44047.1"
                     /translation="MAKGRCMKCQKEVEIKDGKEIVMKNGMKAMKGVCPKCSTKVFRI
                     LGKAK"
     gene            1047..1178
                     /locus_tag="VFF28_00015"
     CDS             1047..1178
                     /locus_tag="VFF28_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44048.1"
                     /translation="MWNKCESCNKQIQSAEDCRLSTNDGRTIHTYCKECYQKKKQAQ"
     gene            1412..1927
                     /locus_tag="VFF28_00020"
     CDS             1412..1927
                     /locus_tag="VFF28_00020"
                     /inference="COORDINATES: protein motif:HMM:NF014102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44049.1"
                     /translation="MVLTNKKIHGLINEIAGEKAIPIIEYLKDRKNVSEFIIADKVKL
                     DMQTTRNILYTLNSYNVATYIRRKDRKKGWYISYWTFNRKRVKELTEQLREEKIVKLK
                     ERLYKEETNKGNFFICSQACARLDFEQATEFGFKCPECGSLLNQQDNEKTIDNLRTKI
                     TELEAARNESS"
     gene            1936..2529
                     /locus_tag="VFF28_00025"
     CDS             1936..2529
                     /locus_tag="VFF28_00025"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZX44050.1"
                     /translation="MDNVIDVIKNRRSIRKYVNRPLLKSEIEQIIDAGRYAPSAHNAQ
                     PWKFIVITDKAKINYLSEEIKGWFRIRARLAKIVRVFNAKVSRELETAEKRIVEEDLF
                     FYNAPVLVLICAKPGRFSLVDCACAGQNMMLVARSLGIGSCWIGYADLVINKKRNVMR
                     ELGVPEDHKVMGHLIFGYPIKFPEKALPRKMEEIKWI"
     gene            complement(2516..2929)
                     /locus_tag="VFF28_00030"
     CDS             complement(2516..2929)
                     /locus_tag="VFF28_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44051.1"
                     /translation="MVYPIRSYHQSQRDRKLHVVSVVELRDVYEILSSYKREKVGREL
                     HFQMFNVKLDGTTGVTKLHIRKHSVSAEQVLGLLSKLEDKYGPLYVEAGYEIYLGKNK
                     TEAHYIDLTQKSVPGKQAIPKLSMTIDWLMYSRST"
     gene            complement(3128..3670)
                     /locus_tag="VFF28_00035"
     CDS             complement(3128..3670)
                     /locus_tag="VFF28_00035"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZX44052.1"
                     /translation="MLRALCEKLFFSDFYAHSALWKRDNNPIGRFYERLQDSGAGLGD
                     TQEISRRYFSELLDYTQLGNHITALDIGCGEGDFIDYLTLDLPHVSLDDCLGVDLVQS
                     NIAVARAKHPGNRFVQDSFLNHRFERKYDLVIAKGVLESRTHFYERYLEGAIRKMLSL
                     SNRYVAFNVITHIEKGALII"
BASE COUNT         1247 a          709 c          827 g         1063 t
ORIGIN      
        1 tataatgaag gaatctctat gccattaaat aatttatcac agaaacctat atatagctaa
       61 agcaaatcaa agcatactat gatcaaagca gtgctttttg accttgacaa cacacttata
      121 gacttcatgc gcatgaaaag aaacagctgt gaggctgcaa tctctgcgat gatagatgca
      181 gggcttgata taaacaagga ggcagcgcta aaaaaactat ttgtgctcta tgacaagcat
      241 ggcatggaag acagcagcat cttccagaaa tttctgaagg aaacaaccgg agagatagat
      301 tataggatac ttgcaaatgc cattgtctcg tatcgggctg taagggcatc atatcttcag
      361 ccttatccac atacggattt tgtgctgata aagcttaagg caaggggagt aaagcttgcc
      421 atagtcactg acgcacccaa gcttaaagca tgggtaaggc ttatgagcat gaaaatcggg
      481 aatttctttg atgtcgtggt cgcatttgaa gataccaggc agctaaagcc atcaaacctt
      541 ccgtttaatg ctgctatagg aaagctggat gttaaacctc aagaatgcct gatggtagga
      601 gacatgccaa atagagacat agctggtgca aaaaagcttg ggatgaaaac atgctttgca
      661 aggtatggga atgataaaat aagaaagagc aatgctgatt atactataga tgatataaag
      721 gaccttctta agattgtcaa atagacgata aagcaaccaa acatttatat actaagccgt
      781 cgaagagccc ccaatagggg gatttggaat ccttagaggt gttaaaaaat ggcaaaagga
      841 cgatgtatga aatgccagaa agaagtggag ataaaggatg gcaaagagat cgttatgaaa
      901 aacggcatga aggctatgaa aggagtatgc cctaagtgca gcacaaaagt attcaggatc
      961 cttggaaagg ctaagtaagc ctttttccct tttttatcat aacatttata tatttctctt
     1021 aagactcatt tagaggggat tatcaaatgt ggaataagtg cgaatcctgc aacaagcaga
     1081 tccagtctgc agaggattgc aggttatcta ccaatgatgg caggaccata cacacatact
     1141 gcaaagagtg ttatcaaaag aaaaaacaag cccaatagtt atacttaaag atgacttttg
     1201 gcgattttta taacttctga gaagcaaaaa ccgaaagttt tatataatag cttatctaaa
     1261 ggtgcaatat atagatactt ttctaggggg tggaagggca gagccttaga aaagccaata
     1321 atcagtagca atataagccg aaagctggat aacagagtgg ctgtgagtga caccgttact
     1381 gacatatcag gattagactg gataatgcaa aatggtacta acaaacaaaa agatacatgg
     1441 actaatcaac gagatagctg gggaaaaagc aatacctatc attgagtatc ttaaggatag
     1501 aaagaatgtt tctgagttta tcatagctga caaggtaaag cttgacatgc agacaactag
     1561 gaacatactc tacacgctta attcttacaa tgtcgctaca tatataagaa ggaaggacag
     1621 gaagaaagga tggtatataa gttactggac cttcaacagg aagagggtca aagagcttac
     1681 agagcagctt agggaagaaa agatagtaaa gttaaaagaa aggctatata aggaagagac
     1741 gaacaaaggg aattttttta tatgctcaca ggcttgtgcc aggcttgatt ttgaacaggc
     1801 gacagaattt ggctttaaat gccctgaatg cggatcgctt ctaaaccagc aggacaatga
     1861 gaagacaata gataacttaa ggacaaagat cactgaactg gaagcagcaa ggaacgaaag
     1921 ttcctaaggt ttcttatgga caatgtcatc gatgtcatca aaaataggcg cagcattagg
     1981 aaatatgtca atcggccttt gcttaagtca gagatcgagc agataatcga tgctggaaga
     2041 tatgcacctt ctgcacataa tgcgcagcct tggaaattca tagtcataac cgataaggct
     2101 aagataaact atctttctga ggaaatcaaa ggctggtttc ggattagggc aaggcttgcc
     2161 aaaattgtac gagtgtttaa tgccaaggtt tcccgtgaac ttgaaacagc agaaaaaagg
     2221 atagttgaag aagatctgtt cttttataat gctcctgtcc ttgtgcttat atgtgctaaa
     2281 cccggacgat tttcccttgt tgactgtgca tgcgcaggcc aaaacatgat gcttgttgca
     2341 agatcccttg gaataggctc atgctggata ggctatgctg atcttgtcat caataagaaa
     2401 aggaatgtga tgagagagct tggagttcct gaagaccata aggtgatggg gcacctcata
     2461 tttggctatc ctataaagtt tcctgagaag gctttgccaa gaaagatgga agaaattaag
     2521 tggatctaga gtacataagc caatctatag tcatgctgag tttaggaatg gcttgttttc
     2581 cgggtactga tttctgtgta aggtctatat agtgcgcttc agtcttgttc ttgcctagat
     2641 agatctcata acctgcttct acatataaag gaccatattt gtcttcaagc tttgaaagca
     2701 gccctagaac ctgttctgcg cttacactgt gctttctgat atgaagcttg gtcactcctg
     2761 ttgtcccatc cagcttgacg ttgaacatct ggaagtggag ttcccttccg accttctcac
     2821 gtttatagga ggaaaggatt tcgtatacat cccttagctc tacaactgat acaacatgaa
     2881 gctttctgtc tctttgcgat tgatgatatg accttattgg ataaaccata ctttaaagaa
     2941 ggtaaaaaaa ttatttaaga atatgatgtt taataatgcc tttgctaaaa catagaaagc
     3001 gtcttctgaa tctggcatga tttttgagat atggcagtgt atccttgcat ctgaacccct
     3061 tatcttatca actatccggc tgatatctcc gaaagggata gctgtccggt tgcctatctt
     3121 tgaggagtca gataatcagg gcgccttttt cgatatgagt gattacatta aaagcaacat
     3181 atctatttga taagctaagc atctttcgaa tggccccttc caaatatctt tcgtaaaagt
     3241 gagtcctaga ttccaggact cctttggcga tgaccaagtc atatttcctt tcaaatctat
     3301 gatttagaaa gctatcttgt acgaatctat tgcccgggtg cttggctctt gcaacagcga
     3361 tattgctctg cacaagatcg acgcctaggc agtcatccag gctgacatga ggaaggtcta
     3421 atgttaaata gtcaataaaa tcgccttctc cacaacctat atctaaggct gttatgtgat
     3481 ttcccaattg tgtataatca agtaattcac tgaaatatct cctgcttatc tcctgtgtat
     3541 ccccaaggcc tgctcctgaa tcctgcagcc tttcataaaa cctgcctatt gggttattat
     3601 ctcttttcca tagggcggag tgggcataaa agtcgctaaa gaataatttt tcacataaag
     3661 cccttagcat tatgaattta taaatctggt taccgctgac gatagcgtgt gcggacactt
     3721 aaaattggtt aagtcgcagc gttctcaatc aaagcattat gcctaaggcc ataatggtgc
     3781 tcaaaatctc cattaatata ttcagccctc agatgcgcca tataatttgc gttttcttcc
     3841 ttccat
//
LOCUS       DASRUK010000002        12359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_508249, whole genome shotgun sequence.
ACCESSION   DASRUK010000002 DASRUK010000000
VERSION     DASRUK010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 12359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12359
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508249"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            363..860
                     /locus_tag="VFF28_00040"
     CDS             363..860
                     /locus_tag="VFF28_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44053.1"
                     /translation="MKPLDTIFIERGIDRETDPDKMKELVADSIDQYLNSSPFVTSVE
                     IEKAINRLYEQGRWRPKDRVACWQRYSRPVANPHIERRAGVKHISLQEAVTKAAEDLL
                     PVRENWAAAISYYTAGEPTMDRFMGGIDVYFKDEQMMMALLKARLTEERRTINYKFTM
                     LPSNS"
     gene            942..1259
                     /locus_tag="VFF28_00045"
     CDS             942..1259
                     /locus_tag="VFF28_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44054.1"
                     /translation="MEQIILIDADKIPEDDKATIQKIASEYIPKIQRVFKDKIMVKLH
                     YKKHGERKYDLRVVINCPDKKLEAQESDWNLRRTLHKVFENIEREMEHKFKLSDQNKP
                     FER"
     gene            complement(1294..1383)
                     /locus_tag="VFF28_00050"
     tRNA            complement(join(1294..1307,1323..1383))
                     /locus_tag="VFF28_00050"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1346..1348),aa:Met,seq:cat)
     gene            1491..1844
                     /locus_tag="VFF28_00055"
     CDS             1491..1844
                     /locus_tag="VFF28_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44055.1"
                     /translation="MVDNKLCQRCGQCCNGLVWRKKRSYKNFPGLGDRSNAEKEIIQT
                     YRQYLDRIGIPVTKILPIEWDEKNKEVMIKAQAGTCRNLSFLPDGKALCLNYAERPNE
                     CRNFICKKAEGLLAK"
     gene            complement(1830..2879)
                     /locus_tag="VFF28_00060"
     CDS             complement(1830..2879)
                     /locus_tag="VFF28_00060"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HZX44056.1"
                     /translation="MVNFQEFFALSFGGNTVRSYIIALAVFFISIALIHLFKTVVIAR
                     LKVICQKTKTQWDDAVINFAEKIGWPFYFMVALYPPLKFLKLPAIADVWLNYGIMIVL
                     IYYSFKGFNGIVDYGTHEVMKKRDQSDAYVIKFFSKLLKVLLWVIAGLLILSNLGYNI
                     NSLMAGLGIGGVAIALALQSVFADLFASVSIYFDKPFKVGDFIMIGETKGEVKKIGIK
                     TTRLQSLWGEEIIISNQELTSSRIQNYKRMERRRIQFKFGVTYQTAQIKLKKIPEIVK
                     EVFEKLKNADLDRVHFKEFGDFSLNFEVAYYVNSSEYNIYMDIQQQINLALKERFEKE
                     GIDFAYPTQTVLLSK"
     gene            3136..3243
                     /locus_tag="VFF28_00065"
     tRNA            join(3136..3150,3175..3243)
                     /locus_tag="VFF28_00065"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3194..3196,aa:Ser,seq:gct)
     gene            3338..3808
                     /locus_tag="VFF28_00070"
     CDS             3338..3808
                     /locus_tag="VFF28_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44057.1"
                     /translation="MKALVAYYSRTGRTKDAAETIANSLKADIEEISEGSSRRGIFGF
                     LKSGREGALKKTPRIKKPLKDIIDYDIAIIGMPIWAGKMASPVRSYVKTYGKEAKQIA
                     LFCTHGGGGGDPALSEIEHIMGKKAIAKIELTTNDTSKINEFSDFVQKTYKSSK"
     gene            3818..5764
                     /locus_tag="VFF28_00075"
     CDS             3818..5764
                     /locus_tag="VFF28_00075"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HZX44058.1"
                     /translation="MKRLCLIAAAILLANLCSAEDWAFRDEHADISIKISGKIDVIPK
                     GSDSTIDHLTANLSLFPQDGLQQEVLTLATEPRAEIQGDNAVFRWEKPPGTVKYILTS
                     NVRTNNNRIMVKEEVPFPLDDIPDSVYEYTKPSQTIDSDDRDVIRQASVIAQGDNDLY
                     SIEFKLGRWIKENIQYNLSTLTESVSQDASWVLQNRQGVCDEITNLFIAMNRALGIPA
                     KFISGVAWTNALESGEGFGPHGWAEVYLPGYGWTPYDITYGEYGFVDISHVKLKEGVD
                     VDKASTQFQWLSHNMDVRTSVLDIKAEVQGSDGTALSDIAIEATALKNDVAFGSYNII
                     EGRIKNLQDYYNPVELSLSKPNELILAGRRSQAVLLKPNEERSVYWTIAIAGDLEEGY
                     YWTFPVMITSSMNDSAIVMFNSSVNGKFFSKEELEEAISEGEEDKQYAGGIELYCTAL
                     DDEIYIYEKTVVNCSAKNTGNIYLEGLNICQDDECRRIDIGITQQETIAFAFEPTIAG
                     MQEISIKATGQDVSKSAFVDITVFDIPELRIDEISYPKEVSYNDMLDISFVLSKKSKY
                     PPQNITIKLNKRQWMVNQLSEDRKFSVKIAGKDLSAGDNKMVVSVGYKDGNGKEYNAS
                     EELTINLANVSLFQRIILFFRHLI"
     gene            complement(5761..6108)
                     /locus_tag="VFF28_00080"
     CDS             complement(5761..6108)
                     /locus_tag="VFF28_00080"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZX44059.1"
                     /translation="MATQLWAIGLVVAACFLGSLGPIMLKKAHKKSFKLGDIIHNHYL
                     ILGFFFYAIGTVLFVPALKGGDLSILYPLVATNYIWVSIWSVKLLKEKMNVNKWLGIF
                     LIIGGVFVIGLAA"
     gene            complement(6110..6478)
                     /locus_tag="VFF28_00085"
     CDS             complement(6110..6478)
                     /locus_tag="VFF28_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44060.1"
                     /translation="MATKTWAIALMVLCTAFTSFAQILYKLGSAKLDLTIAGTILNLP
                     LISGMVLYGLVAIIMIIAFSGGDVSALYPVVATSYVWVALLSVYFFHESLSVLKLSGI
                     AIVIAGIIFISIGSKAPEAA"
     gene            complement(6481..8205)
                     /locus_tag="VFF28_00090"
     CDS             complement(6481..8205)
                     /locus_tag="VFF28_00090"
                     /inference="COORDINATES: protein motif:HMM:NF014416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44061.1"
                     /translation="MSKARQIFTNIRVIILLVALVLAVVAINPNPWNEGVAIRSVVGN
                     SSANLAGIQSPKPNVPPMSREVITAINNRIIKDVDDYNEAIKGLRPNSTFQVKTNKDT
                     YRLIVKERYNLTELNETETKTITEIVPTNQTINGTVVQINKTISRQVVVPKTLSTSLG
                     PEDIGLRVYEAPKTNLRKGLDLQGGTRVLLQPEQKISENDMDNLIDVMTQRLNVYGLS
                     DLVIRKAGDLSGNQYILVEIAGATEDDVKNLLANQGKFEATISNLTVFRGGQDITYVC
                     MSADCAGIDPMSGCGQAQDNQWICNFRFSISLSTEAAQRQADITRNMQVITEDKKQYL
                     DQKLNLILDEKQVDELRIGAELKGKAETNIAISGSGVGLSEQEAAVNALQNMKRLQTI
                     LITGSLPFKLNVVKVDTISPTLGEEFVKNSMLIGLLCILVVSVMLFIRYRKLQISIPI
                     IITLLSEVLMLMGIAALIGWNLDLASIAGIIIMLGTSVNDQVIITDETLMGEGQSRIF
                     NWKERIKRAFFIVVGAYLTITFAMIPLLFAGAGLLKGFAITTIIGVTVGILVTRPAFA
                     NFIEILLK"
     gene            complement(8202..9251)
                     /locus_tag="VFF28_00095"
     CDS             complement(8202..9251)
                     /locus_tag="VFF28_00095"
                     /inference="COORDINATES: protein motif:HMM:NF014416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44062.1"
                     /translation="MDKLVEPEIDLMDAQGKPQKLEVFDKSVNRPPETPVAVAPKVEA
                     QKPIPKDSFSKRLFNFYDKQYKKLMIIPLAILLISIILIGLQVAATGTFINKDVTLKG
                     GVTLTTTQGSGVDAVAIQNSLRSQFPTKDISVREISAAQNSGLIIVSDIDGTNKQELN
                     SFISATEKTVGFKLTDQNYNVEVMGPSLGESFFKETLIALLVAFILMSIVVMIYFRML
                     IPSLIVILCVLSDLIVTLAVVNLVGIRVGTAGIAAFLMLVGYSVDTDVLLTTRVLKRK
                     EGVVMDRVMSSVKTGLMMTATTISALIIGLIFSPSDVIRQIMWIVLIGLFADMLFTWI
                     QNVGILRYYLERKSQ"
     gene            complement(9361..10005)
                     /locus_tag="VFF28_00100"
     CDS             complement(9361..10005)
                     /locus_tag="VFF28_00100"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HZX44063.1"
                     /translation="MDEAEKERVIGDEEPEPKDPWYKGPIRYILMLFLLLTIILWYFP
                     AESIKLDPEPTAIPSLSEVLPDNFKPGNTTIKGDFYSSIQPNDPQLKQLANRISTYSC
                     DGNQVCQAKALYYFVRDNIEYVSDPLNSEYIESPKEVLYTKASDCEGGAILLASLMAS
                     IGIDYELVFIPSHVFIKIKLDKAMKRYKTGDWIYLDWTCKGCRFGEVPLKDKVN"
     gene            10068..11924
                     /gene="dxs"
                     /locus_tag="VFF28_00105"
     CDS             10068..11924
                     /gene="dxs"
                     /locus_tag="VFF28_00105"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HZX44064.1"
                     /translation="METSHFLDMIESPRDLKVLNSNQLRLLANEIRQKIINTVSKTGG
                     HLAPSLGVVELTIALHRVFNCPEDKIVWDVGHQSYAHKLLTGRAKRFHTLRQYKGISG
                     FPRICESKYDSFGTGHSSTSISAALGIAKARDIKNERFKVISVIGDGALTGGMAFEGL
                     NQAGHLKTNLIVVLNDNKMSISPNVGALSSYLRKMITHPKYRQTRKKVERLLKIWSKK
                     AAAKAFALEDTIRALSGPGLLFKEMGFRYYGPVHGHDIDSLIRAFDNIKDVSGPVLVH
                     VITKKGKGYAFAEEEVTKFHGISKFNVANGQKVDAGGNITYTNAFSNALVKLARQDPK
                     IIAVTAAMASGTGLDAFSKEFPERFFDVGIAEQHAVTFSAGLATQGFKPVVAIYSTFL
                     QRAYDQIMHDVCLQGLPVIFAVDRAGLVGEDGATHQGCFDISYLRSIPNMVIMSPKDE
                     DEIGHMLKTAVQLEKPVAIRYPRGAGQGIIVHNPYQVLEFGKAEVLREGDDVLILCAG
                     PLCYDALKAADENRRVSCCVVNSRFLKPLDEELIVSLARRIGKVITIEENSIEGGFGS
                     SVLELFEKNKVKADVKRIGIPDRFIEHGSQEELKREVGLTKKEIEKVIREFT"
     gene            11921..>12359
                     /locus_tag="VFF28_00110"
     CDS             11921..>12359
                     /locus_tag="VFF28_00110"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HZX44065.1"
                     /translation="MTKEIKIGNVKIGNANPVAIQSMTNTLTTDVSGTISQIQRLIDE
                     GCEIIRVSVPDDQSAEALKEISENISIPLVADIHFDHRLAIKSAKYVDKLRINPGNIG
                     KDKIKEVIAAAKEYSIPIRVGVNMGSLEKDIEQKYGFSAQAMVE"
BASE COUNT         3739 a         2447 c         2735 g         3438 t
ORIGIN      
        1 tggtaaagtg tcattgggcc tcacctcttt tttgaggctc atcattttac caacagataa
       61 tcacagaatt agccatccta taagtgttac gcattcatcc ccaaccacaa gtggtgggga
      121 tttctgctaa gaaatttaat caggtcccag caattgtttg taaatgtaat ttcataaagt
      181 gttttcctat ctccttcagc taaccataat cccacacatt ctgctaattt ttctttatcg
      241 cagactaata ccatgcaagc atcgaatagg tttagattaa taaaggtttt tgaaaagaaa
      301 atttcattct tttttgtaat cacaatattt ttaaacttat tagtagcaac cctttgctat
      361 atatgaaacc gcttgatact atatttatag aacgggggat tgacagagaa acagaccccg
      421 ataaaatgaa agagttggtg gcagattcta ttgaccaata tctaaattct tccccttttg
      481 taacatccgt tgagattgag aaagcgatca accgattata tgaacaaggg aggtggcggc
      541 caaaagaccg tgtagcctgt tggcaacgat atagtaggcc cgtcgctaat cctcatattg
      601 aaagacgtgc gggtgttaag catatatctt tacaagaagc cgtaaccaaa gctgctgagg
      661 atttattgcc tgttagggaa aattgggctg ctgctatctc ttattacact gcaggagaac
      721 cgactatgga tagatttatg ggagggatcg atgtatattt taaagatgaa cagatgatga
      781 tggccttatt gaaagcaagg ttaacagaag aaagaagaac cataaattat aagttcacta
      841 tgctgccatc caatagctag cagtcaatat tattttctca aattggctgg cctcattttc
      901 ttttcataaa atatttaaac aagcgattgc catgagatgg tatggaacaa atcatattaa
      961 ttgatgcgga taaaatacct gaagacgaca aagcaacaat ccagaagata gcaagcgagt
     1021 acatacctaa gatacagaga gtgttcaaag acaagatcat ggtaaaactg cattataaga
     1081 agcatgggga gcgcaagtat gaccttaggg tcgtcatcaa ctgccctgac aagaagcttg
     1141 aagcgcagga aagcgactgg aacctgagaa ggacactcca taaggtcttt gagaatattg
     1201 aaagggagat ggagcataag ttcaaattgt ctgatcagaa caagcctttt gagagataag
     1261 ttaattctaa attttatttc aatttaaaaa aagtagcgga gggtggactt tcagatctct
     1321 cttttgaacc accgacctat gggttatgag cccatcgggc actcctgact gccctactcc
     1381 gctatttctc taggaaacat tgagcattta taaaactaac gaaagcaaat atatttaaaa
     1441 tattaaatct actcaaacta aagttgcaaa ttctattttg gaggcttatc atggtagaca
     1501 ataaactgtg ccaaagatgc gggcaatgct gcaacggact tgtttggcgc aagaaaagaa
     1561 gctataaaaa ctttccaggg cttggtgata ggtcgaatgc cgaaaaagag atcatccaaa
     1621 catatcgtca gtacctggat aggataggaa tacctgtgac aaagatactg ccgatagaat
     1681 gggatgagaa gaacaaggaa gtgatgataa aggcacaggc agggacatgc agaaacctta
     1741 gctttctgcc tgatggaaaa gcgctttgct taaattatgc tgaacggcct aatgaatgca
     1801 gaaatttcat ctgcaaaaaa gcagaaggct tacttgctaa gtagaactgt ttgtgtagga
     1861 tatgcaaaat caataccttc tttttcaaat ctttccttta gagcaaggtt aatctgctgc
     1921 tggatatcca tgtatatgtt atactcactg ctgttcacgt aatatgctac ttcaaagttc
     1981 aggctgaaat cgccgaattc cttaaaatgt accctatcaa ggtctgcgtt tttcaatttc
     2041 tcaaatacct ctttaacaat ctcaggaatc ttttttagct ttatctgggc tgtttggtat
     2101 gtcactccaa atttaaactg tatccttctg cgctccatcc gtttataatt ttggatccga
     2161 gaggatgtaa gctcctggtt ggagattatt atctcttccc cccagagact ctggagcctt
     2221 gtcgtcttta tgcctatctt ctttacttcg cctttagtct caccgatcat tataaaatcg
     2281 ccaaccttaa agggcttgtc aaagtagata gaaaccgagg caaagaggtc tgcaaagacg
     2341 ctctgcaagg caagggcgat agctactcca ccaatcccga ggcctgccat aagagagttg
     2401 atattataac ctaagttgga aaggataaga aggcctgcaa tcacccataa caggacctta
     2461 agaagcttgc tgaaaaactt gataacatag gcatcgctct ggtccctttt tttcatcact
     2521 tcatgggtgc cgtaatcaac tatcccattg aatcctttaa aagagtagta aataaggaca
     2581 atcatgatgc cataattcag ccacacatca gcaattgccg ggagctttag aaacttgaga
     2641 gggggataca atgcaaccat gaagtaaaat ggccagccta tcttttcagc aaaatttatc
     2701 actgcatcat cccattgagt ctttgtcttt tggcatatca ctttaagccg ggcaataacc
     2761 acagtcttga aaagatggat aagtgcaatt gagataaaga aaacagcgag agctattatg
     2821 tagcttcgga cagtgtttcc tccaaagctt agggcaaaaa actcctgaaa gttaaccata
     2881 aatctagaag tttttaagta ttatataaaa ttaactgcac tcgccgatgt ttgcttgccc
     2941 ctgccgggac ttctagtcgg cgcacttttc attgggaatt tcccagtgcg ctcaagaatc
     3001 aatggattag gtgacatttt cgcctcacca gccatcaact ggaaataaag atcccgaccg
     3061 ccccaagggc ggggatattt ttaatgttgg agacttacaa aaatttataa aggaatataa
     3121 gattctcaag gatatgccgc ggtggcacaa acaggactat ttttcctggt gcagcctggt
     3181 actgcgcggg attgctaatc ccgtttccga aaggatatcc cggttcaaat ccgggccgcg
     3241 gcgtttaatt tctaatgttt accagcaaca tatggctcaa atactgattt atgtaattgg
     3301 taaaatttaa aaggttaaaa cacttataat ggtcactatg aaagcgcttg tagcctacta
     3361 ctcaaggacg ggaaggacga aggatgctgc tgagaccatc gcaaactcgc tcaaagcaga
     3421 tatcgaagag ataagcgaag gcagttcaag aagaggcata tttggattct taaaaagcgg
     3481 cagagaagga gcgcttaaga aaactccaag gataaaaaag ccgcttaagg atatcataga
     3541 ttatgatata gcaatcatag ggatgccgat atgggctggc aagatggcaa gccctgtcag
     3601 gtcttatgtc aaaacctatg gaaaagaagc aaaacagatc gcattgttct gcacacacgg
     3661 cggaggtgga ggagatccag ccctttctga aattgaacat atcatgggca aaaaagcaat
     3721 agcaaagatc gaactaacca cgaacgatac aagcaaaata aatgagttct cggactttgt
     3781 gcaaaaaact tataaatcaa gcaaataaga gcaggttatg aaaagattat gccttattgc
     3841 tgctgcaata ttattagcaa atctatgttc tgctgaggat tgggcattta gggatgaaca
     3901 tgcagatata agcatcaaga tctccggcaa gatagatgtc atcccaaaag gctctgacag
     3961 cacaatcgac catcttactg caaacttatc tctttttcct caggatgggc tccaacagga
     4021 agtcttgacg cttgcaaccg agccaagggc agaaatccaa ggggacaacg cagtattcag
     4081 gtgggaaaaa ccgcctggaa ctgtcaaata tatcttaaca agcaatgtta ggacgaacaa
     4141 caatagaata atggtcaagg aagaggtacc ttttccgctg gatgatatcc ctgactcagt
     4201 atatgaatac acaaagcctt cgcaaaccat cgattctgat gacagagatg taatcaggca
     4261 ggcttcggta atcgcccaag gagacaacga cctctattct atcgaattca agctgggcag
     4321 atggataaag gagaacatac aatacaacct aagcacccta actgaatcag tgtcacaaga
     4381 tgcttcctgg gtgctacaaa atagacaggg agtatgcgat gagataacaa acctgtttat
     4441 cgctatgaac agggctcttg ggatacctgc aaagtttatc tccggggtcg cctggacaaa
     4501 tgcccttgaa tctggagaag ggtttggacc tcatggatgg gcagaggtat atcttccagg
     4561 atatggctgg acaccttatg atataactta tggtgagtat ggctttgtag atatatctca
     4621 tgtaaagcta aaggaagggg tagatgtaga caaggcaagc acacaattcc aatggctgtc
     4681 acataatatg gacgtgagga catcagtgct ggatatcaaa gcagaggtgc aggggagtga
     4741 tggcacagct ctaagtgata tcgcaataga ggcaacagcg ctaaaaaatg atgtcgcttt
     4801 tggcagctac aacataatcg agggaaggat aaagaatttg caggattatt ataaccctgt
     4861 ggagcttagc ttgtcaaagc ctaatgaact catcctagca ggcaggagaa gccaagcagt
     4921 cctcttaaaa ccaaatgaag agagatcagt gtattggaca atagctattg ctggggatct
     4981 ggaagaaggg tattactgga cgtttcctgt aatgattacc tcatccatga acgacagtgc
     5041 gatcgtcatg ttcaactcgt cggtgaacgg caagtttttc agcaaagaag agcttgagga
     5101 ggccatatct gaaggtgaag aggataaaca atacgctgga gggattgaat tatattgtac
     5161 tgcgcttgat gacgagatct atatctatga aaagacagtg gtgaattgtt cagccaagaa
     5221 tacaggcaat atatatcttg agggacttaa tatctgccaa gatgatgaat gcagaagaat
     5281 agacattgga atcacacagc aggagactat agcgtttgct ttcgagccaa ccatagcagg
     5341 catgcaggag atatctatca aggcaaccgg ccaagatgtg agcaagtcgg cttttgtcga
     5401 tatcacagtc tttgatatcc ctgagctaag gatcgatgaa ataagctatc caaaagaggt
     5461 gtcgtataat gatatgctgg atatatcttt tgtattaagc aagaaatcaa aataccctcc
     5521 ccaaaatatc acgataaagc ttaataagag acaatggatg gtaaaccaac ttagcgaaga
     5581 caggaaattc agcgtcaaga ttgcaggaaa agacctttct gcaggtgaca ataagatggt
     5641 agtctctgta ggctataagg atggcaatgg caaagaatat aatgcaagtg aagaactcac
     5701 catcaaccta gcaaatgtca gtttgttcca aaggataata ctcttcttca ggcaccttat
     5761 ctaagctgca aggccgataa caaacactcc acctattatc agaaagatgc ctaaccattt
     5821 atttacgttc atcttttcct taaggagctt taccgaccag atggataccc agatataatt
     5881 ggtagccaca agagggtata gtattgacag atctccgcct tttagagccg ggacaaaaag
     5941 gactgttcct atagcataga agaaaaagcc aagtataaga taatgattat ggattatatc
     6001 tcctagcttg aacgattttt tgtgtgcttt ttttagcatt attgggccaa gcgagccaag
     6061 gaagcacgct gcaacgacaa ggcctatcgc ccaaagctgg gtcgccatgt tatgctgcct
     6121 ccggtgcttt tgagccgatg ctgatgaaga ttatgcctgc tatcactatg gctatgcctg
     6181 acagttttaa tacacttaga ctttcgtgaa agaaatatac tgagaggagt gctacccaaa
     6241 catagcttgt cgcaacaaca gggtataagg cagagacgtc cccgccactg aatgcgatta
     6301 tcattattat cgctacaagc ccgtaaagga ccatgccaga aataagggga aggttaagga
     6361 tagttcctgc aattgtcaag tcaagctttg ctgaacccag cttgtatagt atctgggcaa
     6421 aggaagtaaa ggctgtgcac aaaaccatca acgctatggc ccaggttttg gtcgccattt
     6481 ttatttaagc agaatttcta tgaagtttgc aaacgccggc cttgtaacaa gtattccgac
     6541 ggtcactccg ataatagttg ttatggcaaa gccttttagg aggcctgctc ctgcaaacaa
     6601 gagagggatc atggcaaaag tgatggttaa gtaggcgccg acaacaatga agaaagcccg
     6661 tttaatcctt tctttccagt tgaatatcct tgactgacct tcacccataa gagtttcatc
     6721 tgtgattatg acctggtcgt ttacgcttgt tccaagcatt ataattatcc ctgcgataga
     6781 tgcaagatcc aggttccagc ctatcaatgc agctatgccc ataagcataa gaacctcgga
     6841 aagcaaggtg atgataatag ggatagatat ctgaagcttc ctatacctta tgaacaacat
     6901 cactgaaacg acaaggatac acaagaggcc aatcagcatg gagtttttta caaattcttc
     6961 tccgagggtt ggggagattg tatctacctt tacaacgttt aacttgaacg ggagcgaacc
     7021 tgtgattagg atggtctgaa ggcgcttcat gttctgcagt gcatttaccg ctgcttcctg
     7081 ctctgaaagg ccgacgcctg agcctgagat agcgatattt gtctctgctt ttcccttaag
     7141 ctctgcacct atccttagct catcaacctg tttctcatca agtataaggt taagtttctg
     7201 atcaagatac tgctttttat cttcagttat gacctgcata tttctagtta tatctgcctg
     7261 cctttgagct gcttctgtag aaaggcttat cgaaaaccta aagttgcata tccattggtt
     7321 atcctgagcc tggccgcagc cgctcatcgg atctattcct gcgcaatcag ctgacatgca
     7381 tacataggta atatcctggc cacccctgaa tactgtaagg tttgagatag tagcttcaaa
     7441 cttgccttgg tttgcaagaa gattttttac atcatcttcg gttgcacctg cgatttccac
     7501 caagatatat tgattgcctg acaggtcgcc tgcttttctt ataaccaagt ctgagagacc
     7561 atatacatta aggcgctgag tcataacatc tataagattg tccatgtcat tttctgaaat
     7621 cttttgctct ggctggagaa gaacccttgt tccgccctgc agatcaagac ctttccttag
     7681 gttggtcttt ggagcctcat atacccttaa tccgatgtcc tctgggccaa ggctggtgga
     7741 cagtgtcttt ggaacaacta cctgccttga gatggtcttg tttatctgaa ccacggtacc
     7801 gttgatggtc tgattggtcg gtactatctc ggtaatagtt tttgtctcgg tctcatttaa
     7861 ttctgtaagg ttatatcttt ccttgactat caacctgtat gtatccttat ttgtcttaac
     7921 ctggaaggtt gaatttggcc ttaggccttt tatcgcttca ttataatcat ccacatcctt
     7981 tataatgcgg ttgttgatgg ctgtaataac ctccctgctc ataggaggta catttggctt
     8041 tgggctttgt atgcctgcaa ggtttgccga agagttgccg acaacgcttc ttatggcaac
     8101 accttcgttc catggatttg gatttatggc tactactgcc aggactaatg caaccaaaag
     8161 gatgatgacc cttatgtttg tgaatatctg tcttgctttg ctcattggct tttcctttcc
     8221 agatagtatc ttaatattcc cacgttctgt atccatgtga agagcatatc tgcgaagagt
     8281 ccgataagaa ctatccacat tatctgcctt ataacatctg aaggtgagaa gatcaaaccg
     8341 ataataagtg ctgagatggt cgttgcagtc atcataaggc ctgtcttaac agagctcatg
     8401 accctatcca taacaacgcc ttcttttctc tttaaaaccc ttgttgtcag caggacatct
     8461 gtatctactg agtaaccgac caacataagg aatgctgcaa tacctgctgt gcctaccctt
     8521 atgcctacaa gattgacaac cgctaatgta acaatgaggt ctgataaaac acacaatatg
     8581 actatcaagc ttgggataag catcctaaag tagatcatga caacaatgct catcagtata
     8641 aatgctacaa gaagagctat cagggtttct ttaaagaagc tttctcccaa cgaagggccc
     8701 attacttcaa cgttgtagtt ttggtcagtc agcttgaagc cgacggtttt ttctgtggca
     8761 ctaataaatg agtttagctc ctgcttgttt gtgccatcaa tatctgaaac gataataagg
     8821 cctgagtttt gagctgctga tatctccctt actgatatat ccttggtcgg aaactgagat
     8881 cttagggaat tttgtatggc cactgcatca acgcctgaac cttgagtggt cgtaagggtc
     8941 actcctccct ttaatgtcac atctttgttg ataaaagtac ctgtggctgc aacctgcaga
     9001 cctatcagga taatcgatat cagtaagatt gccaaaggta ttatcataag ctttttgtat
     9061 tgcttgtcat aaaagttgaa gagtcttttt gagaatgaat cttttggtat cggtttttgg
     9121 gcttctacct ttggagccac tgcaacaggt gtttctgggg gcctgtttac cgatttgtca
     9181 aatacttcaa gcttctgcgg ctttccctgt gcgtccatca agtctatctc tggctcaacc
     9241 aatttatcca ccctttggtt ttgagaaaga tgggtaattt aaaaagcttt agttttttaa
     9301 tgaaggtaat tagttatcga ttttagtaag cttgaaagtt gatcaagata ttaaaaaact
     9361 ctaattcact ttatccttca gcggaacttc tccaaaccta cagcccttgc atgtccaatc
     9421 aaggtatatc cagtcgcctg tcttgtatct tttcatggct ttatcaagct ttatcttgat
     9481 gaagacatgg cttgggatga agacgagctc gtagtcaatg ccgatagagg ccataaggga
     9541 ggcaagaagg attgctcctc cttcgcagtc tgatgccttt gtgtataaga cttcctttgg
     9601 gctttcgatg tattcggagt taagcgggtc tgagacatat tctatgttgt cccttacaaa
     9661 ataatatagg gcttttgcct ggcaaacctg gttgccatca caactatagg ttgaaatcct
     9721 gtttgccagc tgcttaagct gagggtcatt tggctggata ctactgtaaa aatcaccttt
     9781 aatcgtagtg tttcctggtt tgaagttatc cggcaagacc tctgataatg aagggatagc
     9841 tgtcggttct gggtctagtt ttatcgattc ggccggaaag taccataata taatagtaag
     9901 gagaaggaag agcatgagga tatatcttat cggaccttta taccaggggt cttttggctc
     9961 tggctcttca tcgcctatca cccgctcttt ttctgcctca tccatgagat taccctaatt
    10021 caacaacctt tataaatatt aacagtaccc tgtaagatgg gggagcgatg gaaacaagcc
    10081 attttttgga catgatagaa agccctagag acctaaaggt cctcaacagc aaccagctta
    10141 ggcttcttgc aaacgagata cggcagaaaa taatcaatac tgtatcaaaa acaggcgggc
    10201 atcttgcacc ttcccttggt gttgtcgagc ttactattgc actgcatagg gtgtttaact
    10261 gccctgaaga caagattgtg tgggatgtcg gacatcaatc gtatgctcac aagcttctta
    10321 caggaagggc aaagagattt catactttgc ggcagtataa gggcatctcg ggctttccaa
    10381 ggatatgcga aagcaagtat gattcttttg gaacagggca ttcaagcact tcgataagcg
    10441 ctgcacttgg aattgccaaa gcaagggata ttaagaatga gagatttaag gtaatctctg
    10501 ttatcggaga tggtgcactt acaggcggga tggcttttga aggcttaaac caggcagggc
    10561 atcttaagac aaaccttatc gttgtgctta atgacaacaa gatgtctatc agcccaaatg
    10621 tgggcgccct ttcttcttat cttaggaaaa tgataactca tcccaagtac cggcaaacac
    10681 gaaagaaagt agaacggctt ctaaagatat ggagcaaaaa ggcagctgca aaagcttttg
    10741 cgcttgagga tacgataagg gctttgtctg ggccgggctt attgttcaaa gagatgggat
    10801 ttagatatta tggacctgtc catgggcatg acatagattc tttgatacgc gcttttgaca
    10861 atatcaagga tgtatccggg cctgtgcttg tgcatgtcat cacaaaaaag ggcaagggtt
    10921 atgcttttgc tgaagaagag gtaacaaagt ttcatggcat aagcaaattt aatgttgcta
    10981 acgggcaaaa ggtcgatgct ggggggaata taacctatac aaacgctttt tcaaacgctc
    11041 ttgtaaaact tgcaaggcaa gatcctaaga taatcgcagt gactgctgcc atggcttctg
    11101 gaacagggct tgatgctttt tcaaaggaat ttcctgaacg cttttttgat gtaggaattg
    11161 ctgaacagca tgctgtaact ttttctgcag ggcttgcaac acaggggttt aagcctgttg
    11221 ttgcaatata ttcaactttc ttacaaagag cctacgacca gataatgcat gatgtttgcc
    11281 tgcaggggct tcctgtcatc tttgcggttg atcgggctgg gcttgtggga gaagatgggg
    11341 caacacatca aggatgcttt gatatcagct acctgagaag catacccaat atggtaataa
    11401 tgtctccaaa ggatgaagat gagatcggcc atatgcttaa gacagcggta cagttggaaa
    11461 agcctgttgc tataaggtat ccgagaggcg cagggcaagg aattatagta cataatcctt
    11521 accaagtttt ggaatttgga aaggcagaag tgctaagaga aggagatgat gttttgatac
    11581 tttgcgctgg accattatgc tatgatgcac tgaaggctgc tgatgaaaac aggagagttt
    11641 catgctgtgt tgtaaattca agattcttaa aaccgcttga tgaagagctg atcgtttctc
    11701 ttgctagaag gataggtaaa gttataacta tagaagagaa tagcatcgaa ggcggatttg
    11761 gcagctctgt gcttgaactt tttgagaaaa ataaggtgaa ggcagatgtt aaaaggatag
    11821 gcatacctga cagatttatc gagcatggaa gccaggaaga acttaaaaga gaggtagggc
    11881 tgacaaaaaa agagatcgag aaagtgataa gggagtttac atgacaaagg agatcaagat
    11941 agggaatgta aagataggaa acgcaaatcc agttgcaatc cagtcgatga ctaatacatt
    12001 gactactgat gtttctggaa ctatatctca gatacaacgg ctaatcgatg aaggatgtga
    12061 aattatcaga gtgagtgttc ctgatgatca gagcgctgaa gctcttaaag agatctctga
    12121 aaatatatcc atccctcttg ttgctgacat acattttgat cacaggcttg ctatcaaaag
    12181 cgctaagtat gttgacaagc ttaggataaa tccggggaat atcggtaagg ataagataaa
    12241 agaagtcatc gccgctgcaa aggaatattc aatacctata agggttggcg tcaacatggg
    12301 gagccttgag aaggatatag agcaaaaata cggcttttct gcacaagcga tggtcgagt
//
LOCUS       DASRUK010000003         7887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1010510, whole genome shotgun sequence.
ACCESSION   DASRUK010000003 DASRUK010000000
VERSION     DASRUK010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 7887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7887
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010510"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            321..1502
                     /locus_tag="VFF28_00115"
     CDS             321..1502
                     /locus_tag="VFF28_00115"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HZX44066.1"
                     /translation="MDYEQVVDEIFSIRGSEHVGSFKLGLKKINDLLEKLGNPQKTLR
                     CIHVAGTNGKGSVCAMISQILIEAGYKTGMYTSPHLKDFRERYQINNRMMTKQEAITY
                     YQKVKPHITNQSFFEVITAMAFLYFKEKKIDYLVCEVGLGGRLDATNVITPLLSIITN
                     IGLEHKDYLGGTIEKIAFEKAGIIKENIPVVTGAKGSALKVIRKMAIERHARLFTYGK
                     PDIKYPLNLKGEFQKINSGVVIKAVNVLNDKYNLKISNAQIKKGLARAMIPGRLQFIS
                     KNTLIDCAHNPPAVKTLCKELVKLKKSHDKIILIMGVLKDKDYRLMLDGLAKLSDRIV
                     IVKPKIPRALDPRVIAKGLEKNFIIIEDTKKALVYAKKIAGPKDLIVVTGSFYVVGEV
                     V"
     gene            1554..2192
                     /locus_tag="VFF28_00120"
     CDS             1554..2192
                     /locus_tag="VFF28_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HZX44067.1"
                     /translation="MVFRDRFDGAEQLAEKLGAYKGSKDALILAVPRGGLQVGNILAK
                     ELDLPLDIIIVKKIPHPGNPEFAIGGVGINGHIVNEELVKKEGLTKAYIYQEIRDLTD
                     SIKDKYRKYRGDKKPPELKDKIVILTDDGIATGYTLLAAVDLIRKESPKKIVLAIPVA
                     AVDSLGQFRGKVDEIICVDEPFSFLAVSQFYESFPEVNDEEAIKLLQEKWKR"
     gene            2180..2821
                     /locus_tag="VFF28_00125"
     CDS             2180..2821
                     /locus_tag="VFF28_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZX44068.1"
                     /translation="MEEIEILSGKAVLKGNLIVQKGSDSIIIFAHGSGSSRLSPRNSY
                     VADEFNKAGFSTLLMDLLTIEEERRDMLTAQHRFDVDMLARRVISATEWVNHNPATSN
                     FSVGYFGASTGAAAALIAAAQTPGIDAIVSRGGRPDLAMRFLPKVDAKVLLIVGGNDP
                     EVIGMNKEALKHLNEESKLEVIEGATHLFEEPGKMEEVARLAEEWFLEHIGRG"
     gene            3024..3230
                     /locus_tag="VFF28_00130"
     CDS             3024..3230
                     /locus_tag="VFF28_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44069.1"
                     /translation="MSAGTYEQFLSEVVIRTLLGESGNIDLLPATEKVEADLQRLLHE
                     YLKPEKPLRVIRTVDYCKCRNCEA"
     gene            3286..3747
                     /locus_tag="VFF28_00135"
     CDS             3286..3747
                     /locus_tag="VFF28_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44070.1"
                     /translation="MTFDNPCYLEDVVATASDPDRGIIAIRNVMSRHISDEAKGSVAD
                     TVYFVGRKNLTFKWERYSKRPAAITSELGYEGYMDNIVQDIQSQGKPVDIVPGTPEVL
                     ASLRERIHRLKDADPLYVSGESPCCKPGNDYVCRFRYNPPKPSSGAVIRKK"
     gene            complement(3721..4575)
                     /locus_tag="VFF28_00140"
     CDS             complement(3721..4575)
                     /locus_tag="VFF28_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZX44071.1"
                     /translation="MTIIGVDIGGTGIRAGVVLDGRVISKVKLETESSKGKKVVLKNI
                     VLAIRRVMRKDVSAICIGCPGPINAATGLIGKIPNVPLKNVNIKELVVKEFKKKVYTD
                     NDAKCFALAESSLGKGKGYHIVLGCTIGTGFGSGLVIDGKIYHGKCSATELGHMSINF
                     DGPKGKCGNHGCLESYVSVKGILSRAKAPDVKELYDRALKGDKTSIQAFEETGFYLGV
                     GLSNAINIFDPDIIIVGGQISKAWKFFSPKMKETIKERAMFNCRIVKSELKDAGILGA
                     SFLSNNCS"
     gene            4751..6730
                     /locus_tag="VFF28_00145"
     CDS             4751..6730
                     /locus_tag="VFF28_00145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZX44072.1"
                     /translation="MKIYYASQSFYPHIGGVPTYLLNLAKEMANKGNEVVEVHLRPLG
                     EENHGEIKGIEVHRVPKEPIDQNIMEKYSRFKEAVYQECHFNQKLFDKPAQDMEGFAE
                     FNKVNEYFGQEIRTLLEQKPADIVHIHDFQLLFTYKYVPRGTPLIITWHIPFIKSMSR
                     NLSEFLVKNLKEYDKIVFSSPEYIKAATKAGIPKEKLELIFPLANTNRFKPLEIDKAS
                     VKKAYNLPLDSKIILSVQRIDAKSGHEQLIKALHKVSKKVPNAILVFVGEKSMSNLLS
                     QDRERLIERIKRMVDELGLNDKVFFLGNIDYNCLPELYNAADIVSLCSKNEGFGLSIT
                     EGMACGAPIVATRVGGIPLQVKNNKNGFLVEVGDVSSTASSIITLLSDSELKKKMSAS
                     ALKEIEKKFKLEKGIEKHLIMYNDVRKIKNDLHNLEYLNLKNIKAVVTDLDRTITDKP
                     ARQAFYAADYDQGLLDELEGLNIDILLATGRSINYVEKLCRKFSLWRCVIAENGAVLY
                     FPRNGKTITTNTVYMAEAKKIIQAMKLPGMTMGRVIISIDISHFPQVKRAIGGLSDFV
                     KFIKNNNEIMILPIGVDKGVGLQLALRLLNIDVDKVIMIGDGENDIDMFLNPGYKIAL
                     ANADEGLKKLANHVTKSSSTQGMREIIRKLKVSNR"
     gene            complement(6832..7656)
                     /locus_tag="VFF28_00150"
     CDS             complement(6832..7656)
                     /locus_tag="VFF28_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44073.1"
                     /translation="MKRPWSYLKGRNPVILPANPVQQQIASVLSDKPEADKYVPKAFE
                     WTLADLIDTALEQATEENPSNTSIRMEVGRPNYSIEVHYTKREGVPESLYVTMWNCPF
                     SPLDIGGWIAEGIHAYGGRANCLKFIKGSQGRLIITDSVGEEKTRVFGDFSKWEREIW
                     HLLNQFGIDRESGLSGLSNLPQYTNTPIDMDKIAQAYRWAVKNGFSFEIYSVGDNREV
                     RIQTYDHKGANWFIERHDWTIDAATFHQMQEIITTVSGFKDWSPAYIKSEGRRIIR"
BASE COUNT         2474 a         1577 c         1706 g         2130 t
ORIGIN      
        1 gcggaacagc agggcatgta tctgctgagc aagtggccaa gtatattaga gaaaacacaa
       61 gtagctattg atgtgcttac gcacattgtg ggcattcatc taccccacta acgtggggag
      121 acctagccca caaactttta ggggtttagc tggcctcatc ttcctttcag ctgcatttgg
      181 gacgcaggag tatcacacaa taatgggttt caaatctagg gatatctata atcttcctcc
      241 aaacgaaaaa tatctgcctg aattgatggc atggcgcccc cattaagttt attaacaacc
      301 aattcttaat gctgattgat atggattatg aacaagtcgt cgatgagata ttcagcataa
      361 gaggctccga gcatgtagga agtttcaagc tgggtctaaa gaagataaat gatcttctgg
      421 aaaagttagg aaaccctcaa aaaacactca gatgcataca tgtcgcagga acaaacggca
      481 agggaagcgt ctgcgctatg atttcacaaa ttttgataga agcaggctat aaaacaggca
      541 tgtacacctc tcctcatttg aaggacttta gagagcgcta ccagattaac aacaggatga
      601 tgacaaagca agaggccata acttattacc aaaaggtaaa gccccatatt accaaccaat
      661 cattttttga agttatcact gcaatggctt tcctctactt caaggaaaaa aagatagatt
      721 accttgtctg cgaagttggc cttggaggca ggcttgatgc gacaaacgtc atcacccctt
      781 tattatctat aataaccaat ataggtcttg aacacaaaga ttaccttgga ggcacaatag
      841 aaaagatagc ttttgaaaaa gccggtatca taaaagagaa catcccggtg gtcactggtg
      901 ccaaaggaag tgcgctaaag gtcataagaa agatggcgat agaaagacat gcaaggcttt
      961 ttacctacgg aaagcctgat ataaagtatc cattgaatct aaaaggagag ttccaaaaaa
     1021 taaactcagg cgttgtcatt aaagctgtta atgtgctaaa cgataagtat aatctaaaaa
     1081 tcagcaatgc acaaatcaaa aaagggcttg caagagcgat gatccctgga aggctgcagt
     1141 ttatctcaaa aaatacactg atagattgtg cccacaatcc tccggctgta aaaacccttt
     1201 gtaaggaatt agtgaagttg aaaaaaagcc atgataagat tattctgatt atgggtgtgc
     1261 tcaaagataa agattacagg ctgatgctgg atggcctggc aaaactgtca gataggatag
     1321 taatagtcaa accaaaaatt ccaagagccc ttgatccaag agttatcgca aagggattag
     1381 aaaaaaattt tattataatc gaagatacaa aaaaagccct tgtctatgca aagaagatag
     1441 caggccctaa ggatctgatc gtagtgacag gctcattcta tgtggtagga gaggttgttt
     1501 agtcagcgcg cctttagcaa atctttttaa caggataaac cctatgcatc tggatggttt
     1561 tcagggatag gtttgacgga gcagagcagc ttgctgaaaa gcttggggca tataaaggca
     1621 gcaaagatgc tttaatcttg gcagttccaa gagggggcct tcaagtcgga aatatccttg
     1681 caaaggagct tgatctccca ttggacataa tcattgtaaa aaagatacct catcctggga
     1741 atccggagtt tgctatcgga ggagttggca tcaacggcca tatcgtcaat gaagagttgg
     1801 ttaagaaaga agggcttacg aaagcataca tctaccagga gataagggac ctgactgatt
     1861 cgataaagga caaatacaga aagtatcggg gtgacaagaa gcctcctgag ctaaaggata
     1921 agattgttat cctgactgat gatggcatcg cgactggata cactctgctt gcggcagttg
     1981 accttataag aaaggaaagc ccaaaaaaga tagtccttgc aatacctgtt gcagcagttg
     2041 attcgcttgg ccagttcagg ggcaaggttg atgagattat ttgtgtggat gagcctttct
     2101 catttctggc agtaagccag ttttatgaaa gctttccgga ggttaacgac gaggaagcga
     2161 taaaactttt gcaggaaaaa tggaagagat agaaattttg tcaggaaaag ccgttttaaa
     2221 aggtaatctg atagttcaaa aaggttctga ttcgataatt atatttgcgc atggaagcgg
     2281 aagctcaagg cttagcccaa gaaacagcta tgttgctgat gagttcaata aagcaggctt
     2341 ttcaactctc cttatggacc tgctgacaat tgaggaggaa aggagggata tgctcacagc
     2401 acaacaccgg tttgatgtag atatgcttgc tcgacgtgtg atttcagcaa cagagtgggt
     2461 aaatcataat cctgccacaa gcaatttttc agtaggctat tttggcgcaa gcacaggcgc
     2521 ggcagccgcc ctgattgcag cagcgcagac tcctggcatc gatgcgatag tctcaagggg
     2581 aggaaggcct gaccttgcga tgagattttt accaaaagtt gatgcaaaag tcctgcttat
     2641 cgtaggagga aacgaccctg aagtgatcgg catgaacaag gaggcgctca agcatttaaa
     2701 cgaagaatca aagcttgagg taattgaagg cgcaacccat ctttttgaag agccaggaaa
     2761 gatggaagag gtggcaaggc ttgctgaaga atggtttttg gagcatattg gcaggggtta
     2821 ggctggcgca gtacaaactt gccctgcatc ttgaagatgt agtatttgcc gcaactacct
     2881 aggtatgcaa ggataatttt caaaaaaatg atggctatgc atttcacaag aagacttaga
     2941 tggattagca ggcaaagtgt atgctgtcag gcaagactcg acattttgga ataaacaaaa
     3001 gccgcaagat cttcctacag tgattgagcg cagggaccta tgagcaattc ctaagtgaag
     3061 ttgtcatcag gacattatta ggtgaaagtg gaaatataga cctgttgcct gcaacagaga
     3121 aagtagaggc tgatttgcaa aggctattgc atgaatatct aaaacctgaa aagcccctaa
     3181 gagtcatacg caccgttgac tactgcaaat gcagaaattg tgaggcttag gtatattcct
     3241 aaaactcctg gttaatatct atcaagaaaa tcatatggta aaaacatgac ttttgataat
     3301 ccttgctatc ttgaagatgt tgttgctact gcctcagatc cagacagagg cattatcgct
     3361 attaggaatg tgatgagcag gcatatatcc gatgaggcta aaggctcggt agctgataca
     3421 gtttattttg tcggcagaaa aaatttaacg tttaagtggg agagatatag caaacggcct
     3481 gcagctatca cttcagaact aggctacgaa gggtatatgg ataatatagt ccaggatatc
     3541 caaagccagg gtaagcctgt agacatcgtt ccaggaaccc ctgaagtctt agcatcactc
     3601 cgtgagcgca tccaccgctt aaaggatgct gaccccctat atgtatcagg tgaaagccca
     3661 tgctgtaaac ctggaaatga ttatgtctgt agattccgct ataatccacc caaacccagc
     3721 tcaggagcag ttattagaaa gaaatgatgc accaagtatc cctgcatcct taagctcgct
     3781 tttgactatc cggcaattga acattgccct ttccttgatg gtctctttca tttttgggct
     3841 gaaaaatttc catgcttttg atatctggcc gccaacgatg attatgtcag ggtcaaatat
     3901 gtttatcgca ttggaaagcc caactccaag ataaaagcct gtttcttcaa atgcctgaat
     3961 agaagttttg tctcctttga gcgccctgtc ataaagctct ttgacatccg gcgctttagc
     4021 ccttgacaag atccccttca cagatacata gctttcaagg catccatgat tgccgcattt
     4081 tcccttgggc ccatcaaaat tgatgctcat atgcccaagt tcagttgcag agcatttgcc
     4141 gtgatagatt ttgccgtcga tcacaagccc tgatccaaag ccggtcccga tagtacagcc
     4201 aagaacgata tgatatcctt ttcctttgcc aaggctggat tcagcaagcg caaagcattt
     4261 ggcatcatta tcggtgtaca cctttttctt aaattctttg acgacgagtt ccttgatatt
     4321 gacattttta agagggacat taggtatctt tccgataagc ccggtagcag cgtttatcgg
     4381 cccagggcat ccgatacaga tagctgaaac atccttgcgc atgacccgtc ttattgccaa
     4441 aacaatattt ttaagcacaa ctttttttcc tttgctgctt tctgtttcta actttacctt
     4501 gctgatcact ctcccgtcca ggacaacgcc tgccctgatg cctgttccgc caatatcaac
     4561 ccctatgata gtcatgcttc ttaccttagc aaaggtatat ataatcattt tgcttttaag
     4621 cataggtaat atttccgata agagacaaca atgagggtgc agacggtcat gctgcgcatg
     4681 cactaaagga ggttttctat aggaacaacc cgacccttgc accacttcgt ttcattctgg
     4741 gaggctatca atgaaaatct attatgcaag tcagtctttc tatcctcata taggtggcgt
     4801 ccccacatac ctccttaacc ttgcaaagga gatggcaaac aagggcaatg aggttgtcga
     4861 agttcatcta aggcctcttg gagaagaaaa tcacggagag ataaaaggaa tagaggtcca
     4921 tcgtgttcca aaagagccca tagaccaaaa tataatggaa aaatacagca ggttcaaaga
     4981 agctgtctat caggaatgcc attttaacca gaagttgttt gataagccag cccaggacat
     5041 ggaaggtttt gctgagttca acaaggtaaa tgagtatttt ggccaggaga taaggacact
     5101 tcttgagcaa aagccggcag atatagtcca tatccatgac tttcagctct tatttaccta
     5161 caagtatgtt ccacgtggaa caccacttat catcacttgg catatccctt tcataaaaag
     5221 catgtctaga aacctttctg aatttttagt aaaaaatctg aaagaatatg acaagattgt
     5281 cttttcctct ccagaataca tcaaagcagc aactaaagca ggtatcccaa aagaaaaact
     5341 tgaactgatc tttccgcttg caaacactaa ccgtttcaag cctttggaga ttgataaagc
     5401 aagtgtcaaa aaagcataca atcttccatt ggattcaaag attatcctca gtgtacagcg
     5461 catagatgca aaatcaggcc atgagcagct gataaaagcg ctgcataagg tatcaaaaaa
     5521 ggtcccaaac gcaatccttg tctttgtagg ggagaagtcc atgagcaatc ttctttctca
     5581 agatagagag cggttgatag aaaggataaa aaggatggtc gatgaattag ggctaaatga
     5641 caaggtgttt tttcttggca acatcgatta caattgtctg cctgagctgt ataatgcagc
     5701 agatattgtt tccttatgct caaaaaacga aggcttcggc ctttccataa cagaaggtat
     5761 ggcctgtggt gccccgattg tagcgaccag ggtaggaggc atacctcttc aggtaaaaaa
     5821 taacaagaat gggttccttg tagaggtagg ggacgttagc tctacagcaa gcagtataat
     5881 cactctcctt tcagatagtg agctgaaaaa aaagatgtct gcaagcgcgc taaaagagat
     5941 agaaaagaag ttcaagctgg aaaaaggcat cgaaaagcat cttatcatgt ataatgacgt
     6001 gagaaagata aaaaatgacc tgcataatct tgaatatctc aatctaaaaa acataaaggc
     6061 agtggttact gatcttgaca ggactataac agataagcct gcaagacaag ctttctatgc
     6121 agcagattac gaccagggtc tcctggatga gctggaaggc ttgaacatcg atatactgct
     6181 tgcaacagga agaagcataa actatgtgga aaaactttgc agaaagtttt ccctttggcg
     6241 ctgtgtcata gcagaaaatg gcgcagtgtt gtattttcca agaaatggaa agacgataac
     6301 cacaaacacg gtctacatgg cagaagcaaa aaagatcatc caggcgatga agcttcctgg
     6361 catgaccatg gggcgggtta tcataagcat cgatatatca catttccccc aggtaaaaag
     6421 agctatcgga ggattgtctg attttgtcaa attcataaaa aacaacaatg agataatgat
     6481 acttcctata ggcgtagaca aaggagtggg tctccagctg gcgcttcgcc tcttaaacat
     6541 tgacgtggac aaggtcataa tgataggtga tggagaaaat gatattgata tgttcctcaa
     6601 tcccggatat aagatagcgc ttgcaaatgc agatgagggt ttaaaaaaat tggcaaatca
     6661 tgtgacaaaa tcatcgtcca ctcagggcat gagggagatt ataagaaagc tgaaggtgag
     6721 taatagatga ctaaatcggg atcagacatt gtttgataat tccattgtat tctattgact
     6781 cttacctatg tattatccta tctgcgagaa atcccattct atccagatat actatctgat
     6841 tatccttctt ccctctgatt taatatatgc cggactccaa tctttaaaac cgcttacagt
     6901 agtgataatt tcctgcattt gatggaaagt ggcagcatca attgtccaat catgcctttc
     6961 tataaaccaa tttgcccctt tgtggtcgta agtctggatg cgcacctccc tattatcgcc
     7021 caccgagtat atttcaaagg aaaagccatt ttttactgcc catctgtatg cttgagcgat
     7081 tttatccata tcgataggtg tatttgtata ctgaggtaaa ttacttaggc ctgataaccc
     7141 tgattccctg tcgatgccga attgattaag taaatgccag atttcccttt cccatttgga
     7201 aaagtcacca aagacgcggg tcttttcttc accaactgaa tcggttatta tcaatcgacc
     7261 ttgagaacct ttaataaatt taagacaatt tgctctgcct ccatacgcat gaattccttc
     7321 agctatccac cctccaatat ccaaaggact aaatggacaa ttccacatag taacgtatag
     7381 ggattcaggt actccctctc tttttgtata gtgaacttcg attgaatagt tagggcggcc
     7441 aacttccatt cttatggaag tattactggg attttcttca gttgcttgct caagcgccgt
     7501 gtctattaag tctgcaaggg tccattcaaa agctttaggt acatatttat cagcctctgg
     7561 tttgtcagat aaaacagagg ctatctgctg ttgcacgggg tttgcaggta agattaccgg
     7621 gtttctccct ttaaggtatg accatggtct tttcatcttc tcaccaaccg ctgaatcatt
     7681 ttaatcacca tcgagtaaat aactgtcaaa tttattataa gcctttgtta taatatatta
     7741 taacaacaat ggtaatcgtt tagctttatc ccactaattc taattatata cttgagcacc
     7801 gaaatattta tttagttctt gtagttacat tgtaactaca tgagaaaacc aattcaaatg
     7861 aagatggaag gttacgaagt tgttgag
//
LOCUS       DASRUK010000004         6381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2181819, whole genome shotgun sequence.
ACCESSION   DASRUK010000004 DASRUK010000000
VERSION     DASRUK010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6381
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2181819"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="VFF28_00155"
     CDS             <1..1028
                     /locus_tag="VFF28_00155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZX44074.1"
                     /translation="IGFPLSGYLLSFLTQDANVLIPATQYLQISFIAIIAMFIYNIFQ
                     SALRGVGEVKLPMFIILVAVVLNFFLDPLFMFGYGFIPAMGVPGVALATLITEYLCAV
                     IGIVVMLKGYGSLKVSFAHMVPRMEWLKKLLRLGLPSSAEFSFRSFGFVLMVFVVSFF
                     GTMATAAYGIGTRIFSFVIIPAIGFALATSSLIGNNLGSKQHDRAEKIAKTGMTVAFM
                     SLTAVGILLTIFAPQISAFFVPGEPELISTSASFIRMMAWSFGLVGAQIVIVGTIRAS
                     GKTKTVMLLVISNTIIQFVLSYVISMTLGYKEIGVWISYPISTVISTTAAYYFYARKD
                     WLRKRIV"
     gene            1321..2565
                     /locus_tag="VFF28_00160"
     CDS             1321..2565
                     /locus_tag="VFF28_00160"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZX44075.1"
                     /translation="MKEFKEFKFCSQLHSAIERLGFSEPTEIQAKSIPLVLEGKDILG
                     ESATGSGKTLAFGCGIIQKVEPKKGLQALILTPTRELAEQVRTVISKLGHGSNLHILS
                     IYGGVSINPQIDKLRTADIVISTPGRLMDHLERGTVSLANVKILVLDEADRMLDMGFI
                     EDIERIIRACPRNRQNLFFSATILPGIERLARNYMNSPTKVFTTNQVDPSKLSQFYYE
                     VPRNMKISLLIHLLKHETSNLVMVFCNTRRSTDLVINNLKANGVSANAIHGGFSQNQR
                     SKAMDQIKSGHTKVLVCTDVAARGIDITNVSHIYNYEIPKDPVDYVHRIGRTARAGAE
                     GKVINLLCDFDYDNFSRVLSDYRFEPQRMEVPAIERVAVRLQERSRRGPQGQGRGFQG
                     QGRGYQGRPGHHGPQRNFHGRR"
     gene            complement(2826..2954)
                     /locus_tag="VFF28_00165"
     CDS             complement(2826..2954)
                     /locus_tag="VFF28_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44076.1"
                     /translation="MKLRYRGIEEHEQKGVIAPKMLSGLLPYFFPAIYLFILLCGM"
     gene            3067..3753
                     /locus_tag="VFF28_00170"
                     /pseudo
     CDS             3067..3753
                     /locus_tag="VFF28_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843189.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
     gene            complement(3826..4440)
                     /locus_tag="VFF28_00175"
     CDS             complement(3826..4440)
                     /locus_tag="VFF28_00175"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HZX44077.1"
                     /translation="MTQNIKPTRSELMELKKRITLAKSGYNLLKKKRDGLILEFFDIL
                     KEVKNVRKDISAEYKESVRKMNLARVMESDLKIKSIALAVSSSPGIEVGQKNVMGVVV
                     PSIGEAAFQKGAFTRGYGLYTSSAVDEAALAYEKVVERIVRAAEIETSMRKLLKEIEK
                     TKRRVNALEFEVIPRLEKSGAYVRLRLEEAERDNVFRLKRIKKK"
     gene            complement(4437..5261)
                     /locus_tag="VFF28_00180"
     CDS             complement(4437..5261)
                     /locus_tag="VFF28_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44078.1"
                     /translation="MLEELMPNFSVDSSGVTLFRGLELSEQTSQLMFVPHIYRGKEPT
                     LQYIYHQIREVAQKLGIDPHLSAASCSDEENSNPDIHRTTSWGRIFRQYAIGNLVLSQ
                     PTSVSLKQGHSSTLFEINGHSGVYKHKDGRILLGKVESQRDNILRPTCGALSHVIKRF
                     LGQEIHNPSEEGVDLVGELEKDLHVYKEEFLNVYHSGRDTDEKLSKGMEYIVTANIKV
                     QTEKTIRYVREAHLAGPAIVFSTMTLNRFKYPETCLLLGVYLIKDGQTYDLTKHPV"
     gene            complement(5366..6381)
                     /locus_tag="VFF28_00185"
                     /pseudo
     CDS             complement(5366..6381)
                     /locus_tag="VFF28_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843143.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
BASE COUNT         1788 a         1424 c         1345 g         1824 t
ORIGIN      
        1 caataggatt tccgctgtca ggttaccttc tttccttctt gacacaagat gcaaatgtgc
       61 ttatcccggc aacccagtac ttgcagatat catttatcgc gataatagcg atgttcatct
      121 ataatatttt tcagtcagcg ctaagaggtg taggtgaagt taagctgccg atgtttatca
      181 tactggtcgc agtcgtccta aattttttcc ttgatccgct gttcatgttt ggttatggat
      241 ttattccagc catgggagtc ccgggtgttg ccttagctac attgataaca gaatatcttt
      301 gcgcagtgat agggattgtt gtgatgctaa aaggatatgg cagtcttaag gtaagctttg
      361 cgcatatggt ccctcgcatg gaatggttaa aaaaactcct taggttaggc ctgccaagct
      421 cagcagaatt ctccttcaga tcttttggct ttgtcctaat ggtctttgtc gtatcatttt
      481 ttggcaccat ggccacagca gcctatggga ttggcacaag gatattcagt ttcgtcataa
      541 tccctgccat aggatttgcc cttgcaacgt cctctctgat tggaaacaac ctgggctcca
      601 agcagcacga cagggcagaa aagatagcaa agacagggat gactgtcgcc ttcatgagcc
      661 tcacagcagt cggcatctta ttgactattt ttgcaccaca gatatctgcc ttttttgttc
      721 caggggaacc agaactcatc tcaacgtcgg caagcttcat aaggatgatg gcttggtcat
      781 ttggccttgt aggcgcccag atagtcatcg tcggcacaat aagggcttca ggcaagacaa
      841 agactgtcat gttgctggta atctcaaaca caataatcca atttgtttta agctatgtga
      901 tttccatgac tctgggatat aaagagatag gcgtatggat cagctatccg atatctactg
      961 tcatttccac cacagcagca tattattttt atgcaagaaa agattggcta aggaagagaa
     1021 tagtttagga cgatatatac gataattaga tatatttata aatagatttg atttctactg
     1081 tattcattaa aagtgcgaca ggaaaaagca cttgttcgaa gagatcattt cgctttttct
     1141 aaaaaaatgt caggaatttt ccatcaagaa tgatggctta aagcaataga tgagttccgt
     1201 gcatgttaaa aaccgaaaaa aacacgttag cactttaagt gcggatttat gcatgccaga
     1261 agaaatccct gataagccta taaacctacg ggttttggca atataattta gaggtataaa
     1321 atgaaagaat ttaaggaatt taagttttgc agccagctgc atagtgcgat agaaaggtta
     1381 ggctttagcg agcctactga gatacaggca aaatccatac cgcttgttct tgaaggtaag
     1441 gatatacttg gcgaatcagc cacaggctca ggcaagaccc tggcatttgg ctgtggaata
     1501 atacagaagg tagagccaaa aaaaggcctt caggcactga tactgacacc gacaagagag
     1561 cttgcagagc aggtaagaac agtcataagc aagcttggcc atggcagtaa tctccatata
     1621 ttatcaatat acggcggagt ctcaataaat cctcagatag acaaattaag gacagcagac
     1681 atagtgattt cgacgccggg aaggctcatg gatcaccttg aaagaggcac agtaagcctt
     1741 gccaatgtta agatactcgt ccttgatgag gcagatagga tgcttgatat gggatttatc
     1801 gaagacatcg agcgcattat cagggcttgc cccaggaaca ggcagaacct ttttttctcg
     1861 gcgacgatac tcccggggat agaaaggctt gcaagaaatt atatgaacag cccgacaaaa
     1921 gtttttacta caaaccaggt cgatccgagc aagctttcgc agttttatta tgaggtccca
     1981 agaaatatga agatctcact tcttatacat ctgcttaagc atgagacatc aaaccttgtc
     2041 atggtatttt gcaacaccag gagaagcaca gacctggtga taaataacct aaaggcaaat
     2101 ggagtgagcg ccaatgcaat acacggcgga ttttcccaaa accaaaggtc aaaggccatg
     2161 gaccagataa agtccggcca cacaaaagtg cttgtatgca ctgatgttgc agcaaggggg
     2221 atagatataa ctaacgtcag tcatatctac aactatgaga ttccaaaaga ccctgtcgat
     2281 tatgtccata ggataggaag gactgcacga gcaggagcag aaggaaaggt catcaacctt
     2341 ctttgtgact ttgattatga taatttctca agggttctta gtgattatcg ttttgagcct
     2401 cagaggatgg aagtgcctgc aatagagagg gtggcagtaa ggctccagga gaggagcaga
     2461 agagggcctc agggacaagg tagaggattc caaggtcaag gtagagggta ccagggaagg
     2521 ccgggtcatc atggccctca aagaaacttc cacggcagaa gataaagaga gcaggataaa
     2581 aaagtgagcc aaggatagtt ttgtataaat acaccgtccc ttggggccga atgggttaat
     2641 atttttgctt tgtagggtat tccaacataa gaaaggcttt gctttcttag tgccagagat
     2701 tatcaatttc tgagcacatt aaaaataccc cgtcccttgg ggcgaatggg atttttttgt
     2761 ttatgattca ataaatatac tgcacatctc tatatcatgg taggcgtcat aggcttcttt
     2821 tggacctaca taccgcagag caggataaaa agatagatag caggaaaaaa atacggcagc
     2881 aagcctgata gcatctttgg cgcaatcact cctttttgtt catgctcttc tatacctcta
     2941 tatctaagct tcatcaaagc aggcatcccc tggcttttat gatgccctca gcctgccaga
     3001 agcagtgata ttaagaaggg cgatgaaact caaggcttat agcaatctga atctcgcact
     3061 cgcgattcag accctacagt tgaagcagcg gtagaaaagt cagctccgat tctcttgaca
     3121 ggaggaggga cattccttgt cttcctcttt ttccattggc ttttccttga gccaaaacac
     3181 tatgggctta ggggagaaga gtttatccac aagcagggca tctggtttta tgcagttgtt
     3241 tccatccttc tcacagcgat agtttggttt tccgtaaaga caaatccttt gatggctttt
     3301 ggcgcagttg taggctctac ggtttttttc atcactcacg gctttaagga aaacgccgta
     3361 aagatggaag aaaagatgct tgaaggtaaa gcaaagatgt cagacctaag caagctgctc
     3421 tatcttgagg taatagacct aactttctcc atagatggag tgataggcgc ttttgcattc
     3481 actctctcta tccttctcat attgattggc aacggcatcg gcgctgtcgt agtaagagaa
     3541 cttacagtaa gaaacataga taaggtaaaa aaatacaaat acctgaagaa cggagcgatg
     3601 tactcgattt ttattttggg cgcgataatg cttatggact catttgggct tcacatacca
     3661 gcatggctct ctccagtcgc cacattcctg ataataggtt atttcttcta taagtctata
     3721 caagcgatta gatcacagca agcaggcgta taagaaagat aacaaaaaga acaacagata
     3781 caagcttgat agaaaaaaac atcagccaaa actttgtttt aatgctcatt ttttctttat
     3841 cctcttgagc ctgaagacat tatccctttc agcttcttca agcctaagcc tcacataagc
     3901 tccggatttc tcaaggcgtg ggattacttc aaactcaagt gcatttaccc ttctctttgt
     3961 cttctctatc tccttaagaa gcttcctcat gcttgtctct atctcagcag ccctgactat
     4021 gcgctctacc actttctcgt atgcaagcgc tgcctcatcc acagcagatg aagtgtacaa
     4081 gccatatcct cttgtgaacg cacccttctg aaaagcagcc tcgccgatag aaggcacaac
     4141 cacgcccatg acgttcttct gccctacttc gatgccagga ctgcttgata cagcaagtgc
     4201 tatggacttt atcttcaggt ctgactccat aacccttgca aggttcatct tcctgactga
     4261 ttctttatac tcagcggaaa tatccttcct gacattcttg acctctttta agatatcgaa
     4321 gaattcaagg ataagcccat ctcttttctt cttaagcagg ttatagccgg atttagcaag
     4381 agttatcctc ttctttagct ccatcaattc tgatcttgtg ggttttatgt tttgggtcat
     4441 actgggtgct tggttaggtc atacgtttgg ccatctttga tcagatacac tccaaggagc
     4501 aggcaagttt ccggatattt aaacctatta agggtcatgg ttgaaaacac gatggctggg
     4561 cctgctagat gtgcctctct tacatagcgg atggtttttt ctgtctgaac ttttatgtta
     4621 gccgttacaa tatattccat ccctttagaa agtttttcat ccgtatctcg cccagaatga
     4681 taaacgttaa ggaattcttc tttatataca tgtaaatcct tttccaattc tcccaccaga
     4741 tcaaccccct cttcgctagg attatgaatt tcttgcccta gaaatcgctt gattacgtga
     4801 gataaagcac cgcaagttgg tctcaaaata ttatctcttt gactctcaac tttaccaagc
     4861 aatatcctcc catctttatg tttataaaca ccagaatggc catttatttc aaaaagggtg
     4921 gatgaatgtc cttgttttag agaaacgctg gtcggctggc tcagaacaag gttgccaata
     4981 gcgtactgtc taaatatcct accccaagat gtagtgcggt ggatatcagg gttgctattt
     5041 tcttcatcag aacaagaggc tgcggataaa tgggggtcta taccaagctt ttgggcaact
     5101 tcccttattt gatgatagat atactgcaag gtgggttctt ttcctctata aatatggggc
     5161 acgaacataa gctgacttgt ttgctcagaa agctccagac ccctaaataa agtaactccg
     5221 ctgctgtcta cagaaaaatt aggcataagt tcttctagca tcaaattacc tccttcttca
     5281 tctcatcatc ggtcattgcc ccctcaagcg gctttttgaa ccttacagtc ccctgtccac
     5341 cagtcacttc ctcaacaagc gctttataga acttcttctt tattgccggt gatatcctga
     5401 caagctcttc ttcaggcata gccccaaaaa gcttccaccc aaggtcaagt gtcccaaata
     5461 tctgcctgtc ctcatccttt gcctgcctta caaacttatc ttcaaattca gcagcaaact
     5521 taagcagcct cttgtccctt gcagacaatg catcttcacc gactatggca acaagccctc
     5581 gcaggtccct tccctctgca tatgctgcat acaattggtc gctgactgcc ttatggtctt
     5641 cccttgtctt tcctataccc aggttcataa gtcttgaaag cgaaggcaag atatctacag
     5701 gaggatatat ccctttccta tgcagctccc tgcttaggac tatctgtccc tcagttatat
     5761 atccagtaag atcagggatt ggatgggtga tatcatctcc aagcatggta aggataggga
     5821 tctgtgttat cgagcccttt tttcccttga tgattcctgc cctctcatag atctgcgcaa
     5881 ggtcagtgta catatagcca ggatatcctc ttctgccagg aacctcttca cgcgcagctc
     5941 ctatctctct tagcgattca caatagttag tcatgtctgt aaggatgacc agcacatggt
     6001 atcctttctc aaacgccata tactcagcag cagtaagcgc catccttggc gtgacaagcc
     6061 tctcaactgc aggatcgtcc gcaagattca aaaatattat cgatcttgga agtgaaccat
     6121 ttgcttcaaa atcagatatg aagcgctggg catcttcgtt cgtgattccc atagctgcaa
     6181 atatgacgat aaagttctcg ccttctccaa ggacctttgc ctgccttgct atctgcaggg
     6241 ccatatcgtt atgtggaagg ccggagcctg aaaacaaagg aagcttctgc cccctgacaa
     6301 gggtgtttgt cgcatctatc gtagatatcc cggtttggat aaaatcagca ggcgatcctc
     6361 ttgagtaagg atttattgct g
//
LOCUS       DASRUK010000005         6549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_343113, whole genome shotgun sequence.
ACCESSION   DASRUK010000005 DASRUK010000000
VERSION     DASRUK010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343113"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..443)
                     /locus_tag="VFF28_00190"
     CDS             complement(186..443)
                     /locus_tag="VFF28_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004557373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35ae"
                     /protein_id="HZX44079.1"
                     /translation="MEGTIKNFKRARHHQAKNQMIVYIPGVASKDEAAKLVGKKVSWK
                     SPAGKEIKGEIRSSHGNKGAVRVLFETGMPGQAIAEKVEIK"
     gene            complement(714..986)
                     /locus_tag="VFF28_00195"
     CDS             complement(714..986)
                     /locus_tag="VFF28_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44080.1"
                     /translation="MRVIGALFLFLISIILVIAGALLTVSIIFIYIGIPLLIFGVILL
                     LISILGIFFGAVKDIVNIVTMPFQKTEKKRSNVIEVKKKDGVYRAK"
     gene            complement(1282..1863)
                     /locus_tag="VFF28_00200"
     CDS             complement(1282..1863)
                     /locus_tag="VFF28_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HZX44081.1"
                     /translation="MNRIDLKKTEEIDNKVNSLCDSGAIKTSHKRVPALIGLMAKKAN
                     NNLKFARASLKISDDKDAKEMLGLDEGDSFYDWVIITAYYSMFHMAHALLATKEIKIG
                     KIRAHEATLYAMAKYFILNGELADELFVIYEDAEAKAEELFNSLSDERNKRGEFTYER
                     LPKTNRLPAVESIKNASQFVRDIGTILKKRRYL"
     gene            complement(1860..2468)
                     /locus_tag="VFF28_00205"
     CDS             complement(1860..2468)
                     /locus_tag="VFF28_00205"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZX44082.1"
                     /translation="MYLTETEIKILGLFGNDIYLELSINAIAKSLGKHYRVIRVNVLS
                     LVKKQLLVSMDLAGAKIISLNLKGGVLSMYMPYVEEISSYNKLFKLLPQIGDIATRAR
                     QIYPMFCLGVFGSYASGTQKKGSDIDLFIICPDGYIKKFKNMVNDFPAIQDMLHWNVF
                     GIEEFREGINSQGTLIYMEIAANKMIIKGAELFYNLIAEVER"
     gene            complement(2521..3027)
                     /locus_tag="VFF28_00210"
     CDS             complement(2521..3027)
                     /locus_tag="VFF28_00210"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HZX44083.1"
                     /translation="MNYSPGLGAAEAGIIAMQLVGDIGVYAFMIMIGGIGTVNFAFSL
                     VTLFTLGKARNGAVAAVLEMMKSITLHQLALFLIVALIAGCIAAMLALWFARLFSKIM
                     CKVNYKWLCLGIIAFICVMALVLTGLVGFLILATSTAVGLIAPLIGVKRSHAMGCLIL
                     PVILFFVL"
     gene            3113..3523
                     /locus_tag="VFF28_00215"
     CDS             3113..3523
                     /locus_tag="VFF28_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44084.1"
                     /translation="MESERKMYTYIKHLELFEKIKVARNLIDESFPGLDGDSLMTMLC
                     HIAHHHYHEKMMDLNEREMFLYDKLLKYGYNPSTVYKWFLLTKVPEDIKGRIKRQEIS
                     QKQAFRMSFNRKRVKEVNLGFQLMEEARAAIRGL"
     gene            3527..4342
                     /locus_tag="VFF28_00220"
     CDS             3527..4342
                     /locus_tag="VFF28_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZX44085.1"
                     /translation="MRLKRKRVKYPNVLNKEQLVKLFDTIDNPKVMVGSFIGFFCGLR
                     IQEVCKLRKQDLDLVNRRLKVIDSKFGKDGYVPIPPQAIRIIQLWLEILGNSEWLFPS
                     NQFKDQHISTKTLYHHFYQALERANLRDFDFNDVKGRPRYKYYFHTLRHSYATYLLEQ
                     GVPIEHISKSLRHEQLETTQIYAHISDIELQKRIDHAFTYPHMNNYQRISSNFSDVPK
                     IEKEEKKIPDKMDFSKITDPIEILKLRFAHGEIPPEDFQKRMSLLKSNLNYIG"
     gene            4344..4940
                     /locus_tag="VFF28_00225"
     CDS             4344..4940
                     /locus_tag="VFF28_00225"
                     /inference="COORDINATES: protein motif:HMM:NF037417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44086.1"
                     /translation="MRYRRKKYGKEILLLYLQRLAKDLGKTPSAFDMNNAKNFPNSST
                     FIAEFGAWNIALRAAGLNLNKLKHYTNEELLQLLRILAERISKSPSSRDMSLAPAFPD
                     SKVFITRFQSWNKALLAAGLEPNRRRKYSDMDLIILLRRLARRLGRSPSYEEANHQRG
                     FPCALTFRERFGTWNNALEKAGLERRRRLIQNADIKAT"
     gene            4918..5604
                     /locus_tag="VFF28_00230"
     CDS             4918..5604
                     /locus_tag="VFF28_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44087.1"
                     /translation="MQILRQLKDSKGRPMRLYLCRQDEKTLLFKEINDSKIIQYELLV
                     QKLAEKLSVRTLKLYSIQGKGILMDYHKDSILLCNYRHGLNPTQKRGLQRIVLLDLLI
                     GNKDRHTANIFVNEHLTAFDHDKILRDIARPSSAFVKLDVGKKLDTDYLEKVEQILQK
                     GNISTKTALLEYFGFNEEDIAMIKTLQDKEIALIVNSLRFSESEKKRIISFLIYRRDN
                     FDCSNWAHTP"
     gene            complement(5636..6196)
                     /locus_tag="VFF28_00235"
     CDS             complement(5636..6196)
                     /locus_tag="VFF28_00235"
                     /inference="COORDINATES: protein motif:HMM:NF015020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIF family HAD-type phosphatase"
                     /protein_id="HZX44088.1"
                     /translation="MEKKVLVVDVDETLLNIEPLFFLRRFRKDYQDYNGKLTNFPGIG
                     KEYYIVPRPRAMEFLTIASLNFELVAFSVVGREITARKLEVLGFKPFFKKIYGKEDLA
                     NNKKSLEAISKDLDVPVSDIVAIDDVPEIFESGIVIPVKPFFMGKHGKYEHEEHEDNL
                     LGAISQALNISQGIKNPDHNKLQGIF"
     gene            6281..>6549
                     /locus_tag="VFF28_00240"
     CDS             6281..>6549
                     /locus_tag="VFF28_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HZX44089.1"
                     /translation="MISRRDFLKASGLTLGGVTASSALTGCDMREKKTKLANGYVARG
                     HTYHSPTDALHYNFEAREVGLTEKSYLDGTVINISEVTIGDRLDRR"
BASE COUNT         2100 a         1380 c         1243 g         1826 t
ORIGIN      
        1 gagttcaacc agacctacaa taattttgtc aaggtttatg gaaaagaaac gccggcaatg
       61 aaggaaggat tagttgataa aaaatgggat tggaatgata ttttctacgc aaaattaaca
      121 ttcgttaatt agcccaatcc cccattttat caattaagct tagacaactg aaagaaaccg
      181 caaacttact tgatctcaac tttctcagca attgcttggc caggcatgcc tgtttcaaaa
      241 agcaccctca cagcgccttt gttgccgtgt gatgacctga tttcgccttt gatctctttg
      301 ccagcagggc ttttccagga aacctttttg cccacaagct ttgcagcctc atccttgctt
      361 gcaacacctg ggatgtatac tatcatctgg ttctttgcct gatggtgtct tgctctcttg
      421 aagttcttga tagttccttc catactcttc tagaacttta cttcggttta aaaagctttc
      481 tttttaagca ggcaaaaagg cagtaatcgc caaaaaccaa acaatcgtga ggaagaggag
      541 agggttgtcc tgtcggaaag tagccccaga ccaatggcct agcgaacatt cattttctgt
      601 tcctctatta ggacattcaa agcatggctg ttatcacctc tgttgttggc ttttctgaat
      661 agaaatatcc aacgtccaag gatgtatatt gtcttggatt tttaattcag atcttatttt
      721 gctctataaa ccccgtcttt cttctttacc tctattacat tagagcgctt cttttccgtc
      781 ttttgaaatg gcatcgtaac tatatttacg atatccttta cagccccaaa aaagattcca
      841 agtatgctta tcaataaaag tatgactcca aagatgagca gaggtattcc gatatatatg
      901 aaaatgatgc ttacggtaag taaagcacca gctataacaa gtatgataga tatcaggaat
      961 aaaaacaatg cgccaatcac tctcataaag ccttgtatta tatttgcctt taaaatagtt
     1021 gctataatct cattttccac cgttgtctga tggtcattgc aagtggaaaa gagaacaaaa
     1081 aatctggcct aatgatggag aaaaaatggt cagatttgtt agataacaaa gaatagtatt
     1141 acaggaagta taaggcatcc catggcatgg cttcttttta cacagataag cgaaagcgat
     1201 aagccaggca gcagtggatg tcgcaagtat cagaaatccg ataagtccag tcagcacaag
     1261 tattacgcaa atagatgtaa atcagagata tcttctcttc ttaagtatcg tgcctatatc
     1321 cctgacgaat tgagaggcat tctttattga ttccactgca ggcagcctgt ttgtcttcgg
     1381 cagcctttca taggtaaact ctcccctctt gttcctttca tcagaaagag aattgaacag
     1441 ctcctcagcc tttgcttccg catcctcata tataacaaac agctcatctg ccaactcgcc
     1501 atttaatatg aagtattttg ccatggcata caatgttgct tcatgggccc ttattttccc
     1561 tatctttatc tccttagtcg caagaagtgc atgcgccata tgaaacattg aataataagc
     1621 agtgattatc acccaatcat aaaaagaatc gccctcatct aatcccaaca tttccttggc
     1681 atctttatca tctgagatct taagggaagc ccgggcaaac ttaaggttat tattcgcttt
     1741 ttttgccatc aggccaatca atgcaggcac tctcttatgg cttgttttta tagccccgct
     1801 atcacaaagg ctatttacct tattatcgat ttcttcagtt tttttaaggt cgattctgtt
     1861 catctctcaa cctctgctat caaattataa aacagctctg cacctttgat tatcatcttg
     1921 ttggcagcta tttccatgta gattagggtt ccctgggaat taattccctc cctgaactcc
     1981 tcgatgccaa atacattcca atgaagcata tcctggattg caggaaaatc gttcaccata
     2041 ttcttgaatt tttttatgta gccgtctgga caaattataa aaagatcgat atcagacccc
     2101 tttttttgag tgcctgaagc atatgatcca aacacaccta agcagaacat gggatatatt
     2161 tgccttgctc tggttgcaat atcccctatt tgagggagta acttaaacaa cttgttgtag
     2221 ctcgagatct cctcaacata gggcatatac atcgataaaa ctcctccttt caggtttaag
     2281 gagattatct ttgcgccagc aaggtccatg cttacaagca gctgcttctt tacaaggctt
     2341 aggacattta ccctaatcac ccggtaatgc ttgccaagac tttttgcaat ggcatttatg
     2401 gacagctcta ggtagatatc attcccaaaa agccccaata ttttaatctc tgtttctgta
     2461 aggtacataa tactccaaag tattatatat attattttga agtattatat aaaactttcg
     2521 ctaaagtaca aagaacaata tcaccggaag tataaggcat cccatggcat ggcttctttt
     2581 cacacctata agcggagcga taagcccgac agcagttgat gttgcaagta tcagaaatcc
     2641 gacaagtcca gtcagcacta gtgccatcac gcagataaac gcgattatcc ctaggcatag
     2701 ccatttgtag tttaccttgc acattatctt agaaaaaagc ctagcaaacc aaagcgcaag
     2761 catggcagct atgcatccag caataagagc aacaataaga aaaagagcaa gttgatgcag
     2821 ggttatactt ttcatcatct caagcacagc agcgacagct ccgttccttg ctttcccaag
     2881 agtaaacaag gtgacaagtg aaaaagcaaa attgacagtg cctatccctc ctatcattat
     2941 catgaaagca tagactccga tatctccgac gagctgcatc gcaattattc ctgcctcagc
     3001 agccccaagc ccaggcgagt aattcactac tctttaataa taatcattag taactactat
     3061 gcaatacaat atcaatacaa accttaaata cgtcgggcaa gggccaatac ccatggagag
     3121 tgagaggaaa atgtacacat acataaaaca tttggaactg tttgagaaga taaaagttgc
     3181 aaggaatctc atagatgagt cttttccggg cttggacggc gactctctaa tgacaatgct
     3241 atgccatata gcccatcatc actatcacga aaaaatgatg gatttgaatg aaagggaaat
     3301 gtttctttac gacaaactgc tcaagtatgg ctataaccct tcaaccgtgt acaagtggtt
     3361 cttattaacc aaggttccag aagacatcaa aggtagaatc aagcgccagg agataagcca
     3421 aaaacaggcc tttaggatgt cattcaacag aaaaagagtc aaagaggtta acttaggctt
     3481 ccaattgatg gaagaggcaa gggcagccat aagggggttg tgaaaaatga ggctgaaaag
     3541 aaaacgggta aaatatccaa acgtactaaa taaggaacag ctggtaaagt tgtttgacac
     3601 aatagacaat ccgaaagtga tggttggttc tttcattgga tttttctgcg gcctacgaat
     3661 ccaggaagta tgcaagctaa ggaaacaaga ccttgatctc gtcaaccgga gattgaaggt
     3721 aatcgacagc aagtttggaa aagacggcta tgtcccgatc cctccgcagg caattcgaat
     3781 aatccagctt tggctcgaaa tacttggaaa cagcgaatgg ctctttccct caaatcaatt
     3841 taaggaccag catatcagca caaagacact ctatcaccat ttctatcaag ccctggaaag
     3901 ggcaaacctc agagactttg actttaacga tgtaaagggc agacccagat ataaatatta
     3961 ttttcacaca ctaaggcaca gttatgcaac ctatcttttg gaacaaggtg tcccgataga
     4021 gcatatctcc aaatccttaa ggcatgaaca gcttgagaca actcaaattt atgcccacat
     4081 tagcgatatt gagctccaaa agcggattga ccatgcattt acataccctc atatgaacaa
     4141 ctaccaacgg attagctcca atttcagcga tgttcccaag atagaaaagg aagaaaagaa
     4201 aatcccagac aaaatggatt tttccaaaat aactgatccc atcgagattc ttaagctacg
     4261 atttgcgcat ggggagatac cccctgagga cttccaaaaa agaatgtctt tgctaaagag
     4321 caacctaaac tacataggct aagatgagat atagaagaaa aaagtatggc aaggaaatac
     4381 ttttgctgta tttgcagcgc cttgcaaaag atttggggaa aactcccagt gcatttgata
     4441 tgaataatgc taagaacttc cctaattcat caacatttat tgccgagttt ggggcatgga
     4501 acattgccct tagggcagca gggctaaact taaacaagct taaacattat accaatgaag
     4561 aattattaca gcttctcaga atccttgcgg aaaggataag caaaagccct tcttccagag
     4621 acatgagtct tgctcctgcc tttccagact caaaggtatt tatcaccaga ttccagagtt
     4681 ggaataaggc tttgcttgca gcaggtcttg agccaaacag gcgaaggaaa tattctgaca
     4741 tggatttaat cattttgtta agaaggcttg ccaggagatt agggagaagc ccttcatacg
     4801 aagaagcgaa tcatcagcga gggtttcctt gtgcacttac ttttagagaa aggtttggca
     4861 catggaacaa cgccttggaa aaagcaggcc ttgaaagaag gaggaggtta attcaaaatg
     4921 cagatattaa ggcaacttaa ggactcaaaa ggaagaccca tgaggctata cctttgcagg
     4981 caggatgaga agacattact attcaaggag atcaatgata gcaagatcat tcagtatgag
     5041 ctgctagttc agaaattagc agaaaagctc agtgttagaa ccttgaaatt atattcaatc
     5101 cagggaaagg ggatacttat ggactaccac aaagactcaa tcctattgtg caattacagg
     5161 cacggcttaa accccacaca gaaaagagga ttacaaagga tagtcctgct tgacctgctg
     5221 attggaaaca aagacaggca caccgcaaac atctttgtca atgagcacct tactgccttt
     5281 gaccatgata aaatcctcag ggatatagcc aggccatctt cagcattcgt aaaattagat
     5341 gtaggcaaaa agctggatac agattatctt gaaaaggttg agcagattct ccagaaggga
     5401 aatatatcaa caaagactgc attgctcgaa tactttggat ttaatgagga agatatcgca
     5461 atgataaaaa cactccaaga caaggaaata gcattgattg taaattccct taggttttca
     5521 gaaagcgaaa aaaagaggat aatttccttt ttaatttata ggagggacaa ctttgattgc
     5581 tcaaactggg cccatacccc ataggtggct acagcttcac aacacagttt gaggcttaaa
     5641 aaataccctg cagcttgttg tggtctggat tttttattcc ctggcttatg tttagggcct
     5701 ggcttattgc acctaaaaga ttatcctcat gctcctcatg ttcatatttt ccatgctttc
     5761 ccatgaaaaa cggcttgaca ggaataacga ttccactctc aaatatctct ggaacatcat
     5821 caattgccac aatatcactt acaggcacat ctaaatcctt gctaatcgcc tcaagggatt
     5881 ttttattatt ggcaagatcc tccttcccat atatcttctt gaaaaaaggc ttaaatccaa
     5941 gaacctcaag ctttcttgct gttatctctc taccaacaac cgaaaaggct accaattcaa
     6001 agtttaagct tgctatcgtt aaaaactcca ttgccctagg ccttggaaca atatagtatt
     6061 ctttgcctat gcctgggaaa ttggtcaatt tgccgttata gtcttgatag tctttcctaa
     6121 accttctcag gaagaaaaga ggctctatat tcaatagggt ttcatcaaca tcaacaacca
     6181 agaccttctt ttccatgcaa taagattact catctgtttt tataaagatt tcgaagagtg
     6241 atgagaaaaa tttataagat aatgattact ccctaaatat atgatttcaa gaagggactt
     6301 tctcaaagca agcgggttaa ctttaggtgg agttacagcc tcaagtgcat taactggctg
     6361 cgatatgagg gaaaagaaaa caaagctggc caacggctat gttgcgagag ggcatactta
     6421 tcactcacca accgatgcat tacattataa ttttgaggca agggaagtag gattaactga
     6481 gaaaagttat cttgatggaa cagtaattaa tatctcagag gtaactattg gtgatcgcct
     6541 tgacagacg
//
LOCUS       DASRUK010000006         3862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1012486, whole genome shotgun sequence.
ACCESSION   DASRUK010000006 DASRUK010000000
VERSION     DASRUK010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012486"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..1211
                     /locus_tag="VFF28_00245"
     CDS             216..1211
                     /locus_tag="VFF28_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HZX44090.1"
                     /translation="MIKQSIKILKGLKHPTGLFSASSIHVKTGYNLSWIRDNIYESLG
                     FEAVNDFNEVRATYRALLDLLFKHEYKIDWAIKEKPKFAYQYIHARYDPITMEEVWDN
                     WGNKQNDAVGALLFKIGELEEKGVKIIRDENDLRILQKLVYYLESIQYWQDRDNGIWE
                     EKEELHASSIGACAAGLNKISKLVEVPEEIISKGEDALSRLLPRESESKDVDMALLSL
                     IYPYNVVSREIALSILENVEKDLVREKGVIRYKGDNYYNNNGEAEWTQGLSWLAIIYN
                     QLGMTDKYTYYMKKAFNAVNEKGELPELYFANSDMHNENSPLGWSQALFLVMQTV"
     gene            1325..1612
                     /locus_tag="VFF28_00250"
     CDS             1325..1612
                     /locus_tag="VFF28_00250"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HZX44091.1"
                     /translation="MIAGKRTFSDLRKEILLCLSTGQHTINQLSYETRINWRTVELHL
                     TYLVGKGLIREAFSSKYARVFELSELGVNYIQLFGVHNVHRNDIVRREIRL"
     gene            1609..2826
                     /locus_tag="VFF28_00255"
     CDS             1609..2826
                     /locus_tag="VFF28_00255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008198679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZX44092.1"
                     /translation="MKVLMFGWEFPPFNTGGLGTACYGLTNGLSRNNVDITFVLPYVT
                     DDTKSGHVNLIAANSISKVRLRGINTKLAGYMTPGQYKRSVLSENLSKRHREIYGENL
                     FSEVCRYALKAGSIAKEELHDVIHCHDWMTYAAGKIAKSVSGKPLVLHVHATEFDRTG
                     GNVNQAVYDLEREGMHAADAIIAVSNYTRKKVIEHYGVSPEKVFVVHNAVDNDSVIES
                     YGSHSIRSSHKVVLFLGRVTLQKGPDYFLLAAKRVLDYEPDARFVVAGSGDMAADMVE
                     KAAELGIGDKVLFTGFLTGKDIERAYQMADVYIMPSVSEPFGLTALEAMKNKTPIIIS
                     NQSGVSEVIGNCLKTDFWDTERMAGLIIGVIRYREAQESLSENGYNEVKKFSWDNPAR
                     KCIEIYNKVMGGR"
     gene            2826..>3862
                     /locus_tag="VFF28_00260"
     CDS             2826..>3862
                     /locus_tag="VFF28_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011526416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HZX44093.1"
                     /translation="MVSVCFYFQVHQPFRLRNYMVFDIGNNRNYHHEEKNREIMAKVA
                     RKCYTPANKLMLELLKNNPEFKISYSFSGVVLEQFEQYAPDVLSSFQELSDTGRVEIL
                     DETYHHSLAFLYSKEEFKEQVKMHNHKIKKLFGQSPSVFRNTELIYNNELANFVEGMG
                     YKGILCEGADHILGWRSPNFLYRPLGAKHIKLLLKNYRLSDDIAFRFSDKGWKEHPLN
                     ADKYSEWINAANGNANCINLFMDYETFGEHQWEETGIFEFMRHLPRKILNHPDNNFMT
                     PSELIKSYEPVSELDIHNMISWADIERDLSAWLGNKMQQSAASSLYQMEKEVKLTNDP
                     SIVDAWRKLQTS"
BASE COUNT         1235 a          696 c          891 g         1040 t
ORIGIN      
        1 tacagtctct taaaaatact tgacgaatga tgaaatttgg ttcttaatgc catctatatc
       61 acctcttgct aattacagaa ggggtactta tataagaact tttctattta acttactata
      121 aacattcctc accaagctga acagagcata ttaaccactt attagtagta taactccata
      181 agatttataa aggcaaccag cgcagtcaaa ctacgatgat aaagcaaagc atcaagatat
      241 taaaagggtt aaagcaccct actggcctgt tttcagcttc gtctatacat gtcaagacag
      301 gctataacct tagctggatc agggacaata tatatgaatc tcttggattt gaggctgtta
      361 atgattttaa tgaggtcaga gctacttaca gagccttact tgacttactt tttaaacatg
      421 aatataagat agattgggct atcaaggaaa aacctaagtt tgcctaccag tatatacatg
      481 ccaggtatga ccctataacc atggaagagg tatgggacaa ctggggcaac aagcagaacg
      541 atgctgtcgg cgctcttttg tttaagatcg gagaacttga ggaaaaaggt gttaaaatca
      601 taagggatga aaatgaccta aggatacttc agaaattggt atactatctt gagagtattc
      661 aatactggca ggacagagac aacggtatat gggaagaaaa agaagaatta catgcttcat
      721 caatcggcgc atgtgctgct ggacttaata aaataagtaa actagtcgag gttcctgaag
      781 agatcatatc gaaaggagaa gatgctttat ctagactact tccaagagaa agtgagagta
      841 aagatgttga catggctttg ttatctttga tctatcccta taatgtcgta agcagagaga
      901 tagcgttgtc aatccttgag aatgtggaaa aggatcttgt gagagaaaag ggagtgataa
      961 ggtacaaagg ggataattat tacaacaaca atggtgaagc tgaatggacg caagggcttt
     1021 cgtggcttgc aataatctac aaccagcttg ggatgactga taaatacacc tactatatga
     1081 agaaggcttt taatgctgta aacgaaaaag gagagcttcc tgagctttat tttgccaatt
     1141 cagacatgca taatgaaaac agccctcttg gatggagcca ggcgctcttt cttgtgatgc
     1201 agacggtatg aactttttct ctatttagca gtaatatcta atcgccccta atatttatat
     1261 ataagtaact gcggagtttc ataatagtgg gaatttgtta tcataatacg cagaggctct
     1321 gcgcatgatt gcaggtaaaa gaacattcag cgatcttcgt aaagagatac ttctatgcct
     1381 gtcaacaggc cagcatacaa taaaccagct ttcttatgag acaaggataa actggagaac
     1441 agtcgagctt catctaacat atcttgtcgg caaaggtctt atcagagaag cgttcagttc
     1501 taagtatgca cgcgtctttg agctttctga gctcggtgtc aactatatcc agctttttgg
     1561 cgtccacaat gtgcatcgca atgacatagt gcggagggag ataaggctat gaaagttctg
     1621 atgtttggct gggagtttcc tccgttcaat acaggagggc ttggaactgc atgctacggc
     1681 cttacaaatg ggctttcaag aaacaatgtg gacatcacat ttgtgcttcc ttatgtcacc
     1741 gacgatacaa aatcagggca tgtaaatctt atagctgcaa acagcatatc aaaggtaagg
     1801 cttagaggca tcaacacaaa gctcgcaggc tacatgactc ctggacaata caaacggagt
     1861 gtgctttcag agaatctcag caagagacat agagaaatat atggtgagaa tctcttttca
     1921 gaggtctgca ggtatgcgct taaggcaggt tctatcgcca aagaagagct gcatgatgtg
     1981 atacattgcc atgactggat gacctatgct gctggaaaga tagcaaaaag cgtctccggg
     2041 aaaccgcttg ttcttcatgt gcatgctact gaatttgaca ggacaggcgg aaatgtaaat
     2101 caggcagtgt atgaccttga gcgagaaggg atgcatgctg ctgatgcgat aattgctgta
     2161 agcaactata cacgaaaaaa ggtaattgaa cattatggtg taagccctga gaaggttttt
     2221 gtcgtgcata atgcagtgga taatgattct gtgattgaga gttatggcag ccattctatc
     2281 agaagcagcc ataaggtcgt gcttttcctt ggaagggtca ctttgcaaaa agggccagat
     2341 tatttccttc ttgctgcaaa aagagtgctt gactatgaac ctgatgccag atttgtggtg
     2401 gcaggatctg gagatatggc tgctgatatg gtcgagaaag cagctgagct tggaataggc
     2461 gataaggttc ttttcacagg gtttttgaca ggaaaagata ttgaaagagc gtatcagatg
     2521 gcagatgtct atatcatgcc atctgtatca gaaccttttg gacttactgc tcttgaggcc
     2581 atgaagaaca agactcctat aatcatatcc aaccaatcag gagtgtctga ggttatcggc
     2641 aactgcttaa agacagattt ttgggacacc gaaaggatgg caggacttat cataggagtg
     2701 atcagataca gggaagctca agagtcgtta agtgaaaatg gttataatga agtaaaaaag
     2761 ttttcatggg ataatcctgc aaggaaatgc atcgagatat acaacaaagt catgggtggt
     2821 cgctgatggt atctgtgtgc ttttatttcc aggttcacca gcctttcaga cttagaaatt
     2881 atatggtctt tgatataggc aataatcgca attatcatca cgaagagaag aatagagaga
     2941 taatggcaaa ggtagcaagg aaatgttata ctcctgcaaa caaactcatg cttgagttac
     3001 ttaagaataa tccagagttc aagatatctt attcattcag cggagttgtg ctcgaacaat
     3061 ttgagcagta tgcgcctgat gtgctttcga gctttcaaga gctttctgat accggcagag
     3121 ttgaaatact cgacgaaaca tatcaccata gccttgcgtt cctatattca aaggaagagt
     3181 tcaaggagca ggtaaagatg cataaccaca agataaaaaa actttttgga caaagccctt
     3241 ctgtatttag aaatactgag ctcatctata acaacgagct tgcaaacttt gtcgagggga
     3301 tgggatacaa agggatatta tgtgaaggcg ctgaccatat actagggtgg agaagcccta
     3361 actttttgta taggcccctt ggagctaagc acataaagct gctattgaag aattatcggc
     3421 tgtctgatga catcgccttt agattctctg acaaagggtg gaaagaacat cctttgaacg
     3481 cagacaaata cagcgagtgg ataaatgctg caaacggtaa tgctaattgc atcaatcttt
     3541 tcatggacta tgagacgttc ggcgagcacc aatgggagga gacagggata tttgagttca
     3601 tgcggcatct gcctaggaag atactaaatc atcctgacaa taactttatg actccctctg
     3661 agctgataaa gtcgtatgag cctgtctctg agctagacat acataacatg atatcgtggg
     3721 cagatataga aagagacttg tctgcttggc ttggaaacaa gatgcagcag tcagctgcaa
     3781 gcagcctata tcagatggaa aaagaagtaa agcttaccaa cgatccttca atagtcgatg
     3841 cctggagaaa gcttcagacc tc
//
LOCUS       DASRUK010000007         2697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_677316, whole genome shotgun sequence.
ACCESSION   DASRUK010000007 DASRUK010000000
VERSION     DASRUK010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677316"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..901)
                     /locus_tag="VFF28_00265"
     CDS             complement(182..901)
                     /locus_tag="VFF28_00265"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HZX44094.1"
                     /translation="MEYRNITVVGTSHIAKQSLDEVAREIESKKPSIIAIELDRKRLH
                     VLFNRKDNKISIYAISKVGLKGYIFALIGAWAEKKLGEHVGVMPGSEMKSAAEIAKKK
                     GIRLALIDQDIEVTLSRLSKSLSWKEKRNFIADIFKGVVLRKKEVEFDLTTVPDKKLI
                     KKLLGQVKKRYPNIYRVLVAERNQVMADNLIKLMKTNEGRILAFVGAGHEEEIITLVK
                     ERINKIDISYTVNVGEHRVSV"
     gene            985..1263
                     /locus_tag="VFF28_00270"
     CDS             985..1263
                     /locus_tag="VFF28_00270"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HZX44095.1"
                     /translation="MLKMKRISETYDMKVFTDSGDYFGEVEESILTANKIFGWRVRAT
                     KNSFLTRVLGSAKGVIVPHQLVRAIGDVMVISKSAVPSYSGEDEDKEA"
     gene            complement(1264..1659)
                     /locus_tag="VFF28_00275"
     CDS             complement(1264..1659)
                     /locus_tag="VFF28_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44096.1"
                     /translation="MRRYVIKQTSILEIMAPHFIAECPIVDYGFKDTPCRHPEYRVET
                     PIGTLDISSLDLKCRGLEWDLVEAVRKGQVFIGIRYNHDGKTTYFFSDQQLARVREVN
                     QESEEWLRKNHLPQNLPKDVEERLRESDF"
     gene            1703..1802
                     /locus_tag="VFF28_00280"
     tRNA            join(1703..1754,1783..1802)
                     /locus_tag="VFF28_00280"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1736..1738,aa:Gly,seq:ccc)
     gene            1846..2421
                     /locus_tag="VFF28_00285"
     CDS             1846..2421
                     /locus_tag="VFF28_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP--2,
                     3-di-O-geranylgeranyl-sn-glycero-1-phosphate
                     cytidyltransferase"
                     /protein_id="HZX44097.1"
                     /translation="MPSPVIVELRKKSFQFFILLIAGLYFILAYSVSRKIALLVLVFI
                     LLLFLFAEYLRLELNMQIPWMNRILKAKEERRMHSGVYFMLSTIICLAVFDARIAVAA
                     LMMSIFGGLAAAIGGRFGKTLLFRDKSLIGSASELGVNILFGLMLLSNAYIILAMAFT
                     ATIAEVLVTDLEDNLFVPLFSGFLGQLIFLL"
BASE COUNT          713 a          583 c          514 g          887 t
ORIGIN      
        1 acctttgaga tgtcagaaac caccactcgc aatctccctg cttcagctcg gagtaagaag
       61 gtggtttctg agcatgctca agaacacgca ccgaggctct tcagcccggc ggttcttgac
      121 aaacaaatca agagttttga agacagtaga gcccccgtca agagcttaat acataccaaa
      181 actaaacact taccctgtgc tctcccacat tcacagtata agagatatct atcttattta
      241 tcctttcttt cactaatgtt attatctctt cctcatgccc tgctccgaca aacgcaagta
      301 tccttccttc attggtcttc atcagcttta tcaggttgtc agccatcacc tggtttcttt
      361 cggcaacaag cactctgtag atgttagggt atctcttttt tacctgccca agcaattttt
      421 ttatgagctt tttgtcagga acagtcgtca ggtcaaactc tacctctttc ttgcgaagca
      481 caacaccttt gaagatatca gcgataaaat tgcgtttctc tttccagctt aggctctttg
      541 aaagtctgct aagagtaact tctatatcct ggtctattag cgcaagcctt atcccttttt
      601 tcttggctat ctcagcagcg cttttcatct ctgagccagg catcactcca acatgctcac
      661 caagtttctt ttcagcccat gctccaatca atgcaaagat atatcccttg agccctacct
      721 ttgagattgc atagatgctt atcttattgt ccttgcggtt gaacaataca tgcagtcttt
      781 tcctgtcaag ctctattgca ataatggaag gcttcttgga ttctatttct cttgcaacct
      841 catccaggct ctgctttgcg atatgggaag taccaacgac agtaatattt ctatactcca
      901 ttggcaggta ggaaaaccgc taagtttata tagtttaggc ttttaatttt aatctactaa
      961 tcatagagct aggaggggat ttcgatgctg aagatgaaaa ggatttcaga aacttacgac
     1021 atgaaggttt tcactgattc tggcgattac tttggcgagg tagaagagtc tatccttaca
     1081 gcaaacaaga tttttggctg gagggtaaga gcgactaaga actctttcct cacaagagtc
     1141 ctgggttcag caaaaggggt tattgtccct catcagcttg taagggctat tggtgacgtt
     1201 atggtcatct caaaatcagc tgtcccttct tattctggtg aggatgaaga caaggaagca
     1261 taattaaaaa tcagattctc ttaatctttc ttcaacgtct ttaggtagat tttgcggaag
     1321 atggtttttt ctcagccatt cctcagattc ttgatttact tcacgaaccc tagctagttg
     1381 ttgatctgaa aaaaagtatg tagttttacc atcatggtta tatctaattc ctataaatac
     1441 ttggcctttt ctcactgcct ctaccaaatc ccattccaat ccccgacact ttaaatccaa
     1501 tgaactaata tccagcgttc caataggagt ttctactcta tattctgggt gtcgacaagg
     1561 agtatcttta aatccataat cgacaatagg gcattcagcg ataaagtgtg gagccataat
     1621 ttcaagaatg ctggtttgtt taataacata tcttctcaaa tttcaataaa tttttattca
     1681 agctattatt tactttatct atacggcagt gatccagtgg ttaagatacg agcttcccaa
     1741 gcttgttacc cgggaaatta ccgcatagcg ataattcggg aattcgaatc ccggctgccg
     1801 tatttcaaca aactttatat agtgcagctt taaggaatag ctattatgcc atcgccggtt
     1861 attgtagagc tgaggaaaaa atctttccag ttttttatac ttctgatagc agggctttat
     1921 tttattttag cctatagcgt ttcaagaaag atcgcattgc ttgtgctcgt ctttatcctg
     1981 ctgctattcc tgtttgcaga atacctaagg cttgaactca acatgcagat tccctggatg
     2041 aacaggattc ttaaagcaaa agaagaaaga aggatgcaca gtggtgtata cttcatgctt
     2101 tcgactataa tctgcctggc ggtctttgat gcaaggattg ctgtcgcagc ccttatgatg
     2161 agcatctttg gaggccttgc agcagctatc ggcggaaggt ttggaaagac attgttgttt
     2221 agggataagt ctttgatagg cagcgcttct gaacttgggg taaatatctt gtttggactt
     2281 atgttattga gcaatgccta tataattctt gcaatggcat ttaccgctac gattgcagaa
     2341 gttctggtaa ctgatcttga ggataatctc tttgtccctc ttttttccgg ctttcttggc
     2401 cagcttatct tcttgctcta ggattttaca caaggcagta ggtgtagaca aaatctggtt
     2461 gctcatagag ctttgtgcaa attttgagcc cctttgcctt gttctgtaac ctaatgaaaa
     2521 acaaatcaaa aagcctattt aaatctatct tttattgggc actgttaact cttcggcata
     2581 catgaaatct ctatcaattt cagcagctta tttcttccca agtcaagttt gatgcctgca
     2641 gcctcggcag gcgtcctgcc tttaagttcc atatttggat tgacaaaatt atggatg
//
LOCUS       DASRUK010000008        11934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_677393, whole genome shotgun sequence.
ACCESSION   DASRUK010000008 DASRUK010000000
VERSION     DASRUK010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 11934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11934
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677393"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..2412)
                     /gene="topA"
                     /locus_tag="VFF28_00290"
     CDS             complement(196..2412)
                     /gene="topA"
                     /locus_tag="VFF28_00290"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HZX44098.1"
                     /translation="MYELIITEKPAAAEKIASALADGKPIRENLGGVPYYKITRGKKD
                     IVVACAVGHLYGLDEKKSSWKFPVFDIEWQPAHESKKSAGFSKKYLMAIKKLCKDANE
                     FTVATDYDVEGEVIGLNVVRYACHKKDASRMKFSTLTKPDLVEAYDNKSKSLDWGQAN
                     AGETRHFLDWFNGINYSRALTSAYKSTGGFKVLSIGRVQGPALKIIVDREKEIKKFIP
                     VPYWQIELNANVNKGEITGWHIKDKFWQKSEADSVMEKVKGQKTGRIASVEKSQFKQQ
                     APNPFDLTSLQVEAYRVFKIQPKDTLAIAQELYTSGCISYPRTSSNQLTPKIGYSKIL
                     SLLSRQGHYSELCSKLLKGKLTPNNGSKTDPAHPAIYPTGNEPHAESRALKIYDLIVR
                     RFMATFGEDAVRETVKANTEVKGEIFVAKGTRTIEKGWFIYYGPYVKLEEEELPKVTE
                     NDTVNIKKITMHDKETSPPKRYTPASIIKELEKRGLGTKATRASIVDTLFQRSYVHGQ
                     QSIEATELGINTIDTLGKYVPNIIDEELTRHFEDEMEQIREGKTNQAVVLGEAKDAIA
                     KVVNDFKKHEKVVGESLKQAFWQAKDTMNTLGKCPACGKGNLMIRKGKFGSFAACSEY
                     PACKTTVSLPRGANIKATKKECEVCKFPIVKELRKKSAKEFCLNPNCKSKNITGEALK
                     EAKAVESGEKKCPKCGGILVIRKSIYGSFLGCSTYPKCKHTEKLESNKTESSKSSP"
     gene            complement(2486..3808)
                     /locus_tag="VFF28_00295"
     CDS             complement(2486..3808)
                     /locus_tag="VFF28_00295"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HZX44099.1"
                     /translation="MARYDRQERLWGQEGQAKLADSTIAIIGEDPFAFPAALNAALVG
                     VNARLFISGIGVEGSYALDFTLKGRNLSAEYTDVLQKINPFVRVSGLPVTFESREDNH
                     FLDGVEAIVDATNNPVSKKVGLEFALQRRIQFISMQSIEGYSVIETGNHTDGQFKGAP
                     KDCCLAWPAGALAIEKAIRFMVYKQIEDEPLFLNPGYTFPFDHTSDAKLPDKNQLSGK
                     TVMVVGVGGQGCFAVPILVESNPARVIYVDHDSVKDHNLSRQPFYWDSVGRYKAEVCA
                     EKHNKMVGGEIAVPLVRKFDEHFDYPEQIDVIYGFTDSMFSRMLISHYCSRKGITFIA
                     AGSDPGGAEFCSYVPGITSCYIHQYLALIESAKQEERIARQGCIDNPNAQVVMPNCFA
                     GSMAVTMSGYDATPFSGRLTYRPSTPAFGRLSKEPCRCHIDGPPDLRI"
     gene            complement(3795..5117)
                     /locus_tag="VFF28_00300"
     CDS             complement(3795..5117)
                     /locus_tag="VFF28_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44100.1"
                     /translation="MNIEELIPDEILISKGVQDYCITTIDIVRRLAGERTGTPQHIEW
                     DSDMVCLRGDNQRIVRGIIPPSESYVGPASVYVPGHAIKANLDEGLKALRQADGADYI
                     VSGILHSHAGFGKGFSGTDYGDFSSEGRRIARDLLVYNRLTLFSLFDAKPYVTIDGRV
                     QITSSESEFDPVVSRVIPADERILGLLKQHGLKRGEGFNPLTFCHELLGLVETEHRQI
                     MQLGIAQAVVVDASRRQPFGIIGYHYSQLFSGPAEGIWRTKEVGVRTVPVENDMRFDE
                     GELAEIMRKSLHICEHPKHHRKKTRSYGTTGGHGIGKYLLSQPPDSAKLAYRFVFTAA
                     GYVGKFGAPDRKYAHYIAGMLGLLDEQGISGAIKSLGPLSQDPEPDRLRLVEYSSATQ
                     AVCKRLTPPNKLELDFVRDFGSADSITGINKVLDQYVPQFLGGRNGQV"
     gene            complement(5114..6211)
                     /locus_tag="VFF28_00305"
     CDS             complement(5114..6211)
                     /locus_tag="VFF28_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44101.1"
                     /translation="MDEILSEHSLLGFGRATHQSNIGDAEKLREIVRHIFSNILPAMY
                     EFGAYRSQFDEGEQKTAVHRVFDRLALSYGEAPSDGIPIPQIVSRIRENTALPNTQCI
                     TSELQYSDPWKSTLAGSFPPAQGNSLLAQIFRGMPYFGHSGKIYRLSPISFFAWNRPK
                     ISSDDREFCLEESADFSTLNGQYAKMLELQLRHQLLDQLKTDSQILNGIRNPEWYIER
                     AIQSGYFEWDFVGFFVYRESRYPSCCVFRRISKFAVVDPLDNNPDPDERRCFFFGETR
                     VGMKVSYTYQIDTGERPFVIDKIDHPADMGDYICNAGSYPGDVANQISEGIATLRHSI
                     TPENSYKFLNKGRQLTVRQARNRGFEVYQRR"
     gene            complement(6459..7115)
                     /locus_tag="VFF28_00310"
     CDS             complement(6459..7115)
                     /locus_tag="VFF28_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44102.1"
                     /translation="MVRKGSKPVISGFADGTGGPNPQYTAPVASIGEDLTAQGEGGAP
                     PDANPMQEVAEQPPASPSSIDAVVGAGSGEKPDQLEVALATAPQKYLLILQATSESGP
                     KREMEIAPDMAVGSPPATLYQMHLYAMGEQVTVGDRLYTPELNREQTRFASYLRLEQQ
                     TGDGKKREWEFILCRPGQQNRSVHGDYVTFHTDSVTLPDGTLQLHAIKVGGRVLGKYR
                     "
     gene            complement(7218..8009)
                     /locus_tag="VFF28_00315"
     CDS             complement(7218..8009)
                     /locus_tag="VFF28_00315"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF009830.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HZX44103.1"
                     /translation="MKIGVIGCGAIGSVLCKFIDKELPQHELVAINDIDEEKAQKLAK
                     SLQKKPNVASIDEVIGDSWLVIEAVNPSVVRSILEKCITKKKHLMVMSVGGVIQNLEL
                     LDKLTARLFVPSGAICGIDGVKAASIDRIDSVTITTSKNPKSLTGAPFIIENKIELDK
                     IKKKTVIFDGNALSAIRGFPKNINVAATLSLAGIGAEKTKVKIVADPSLSTNNHEIEV
                     VGSFGKIYTRTENVVSPLNPKTSHMAVLSACATLKRLTGFLKIGN"
     gene            complement(8006..8938)
                     /gene="nadC"
                     /locus_tag="VFF28_00320"
     CDS             complement(8006..8938)
                     /gene="nadC"
                     /locus_tag="VFF28_00320"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HZX44104.1"
                     /translation="MDRRKILERSFQRGHLLTVKSKFYRDWIDTFIIDEIKADFGKGD
                     ITSEAVLMNKKAKATVFSRSAGVIAGIEEVCYILRSYGLDVRELVKDGEKIRKGSKIL
                     EISGKANSILKLERTALDMLSRMSGIASLTNQLVKKAGKVGIAPTRKTEWRFIDKKAV
                     FVGGGLTHRLALWESILIKDNHLDALKKEGVHDPVVVSIERAWKNRKRGAFIEIEASN
                     EADAFKAAQKFKELQGNSYNTPCFIMLDNMKPAAIKKIIRRLKALNNHVMIEASGGIT
                     PDNLKAYSKTGVDVISMGYLTTSCKALDMKLRFI"
     gene            complement(8972..9868)
                     /gene="nadA"
                     /locus_tag="VFF28_00325"
     CDS             complement(8972..9868)
                     /gene="nadA"
                     /locus_tag="VFF28_00325"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HZX44105.1"
                     /translation="MNELAKKVNELKREKNAVVLGHNYQREEILDVSDFIGDSLELAK
                     KALETKAKIIVFCGVDFMAESAKILNPEKMVLHPEKLSICPMAAMATPEKILEAKKKH
                     PQAAVMSYINTSAAVKAVSDICCTSANSVKVANSLAEYEIIFAPDKNLAAYTQTKTTK
                     KIIPFDGHCYVHDKITAESVRKAKEMHPLAKVMAHPECPMEVINEADAVCSTAQMIKY
                     AKESGASEFIAVTECGMANVLKRLIPDKTFYAAGGTCIQMKKITLDKVYNCLLNETNK
                     VEVPEEIMIKAKLALERMINIS"
     gene            9932..10942
                     /locus_tag="VFF28_00330"
     CDS             9932..10942
                     /locus_tag="VFF28_00330"
                     /inference="COORDINATES: protein motif:HMM:NF020933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HZX44106.1"
                     /translation="MDAISDILDSLKELTKAKGTIVQFNDPSYTPIPISPKNFHKIRE
                     SAGKKTAFIDGGNAEILKSPSFSLQLLRVCSVIFQDNKKVSSRIKECFVLTKAKDIEG
                     KIFYETSFFQEAREKINFDSFDGTIRQGNHRISISSIAGVIRRLLELDLAAETIINLE
                     KGDAIVLDGDLKASITGEKEYLNILLAKAKEKGVIVSALSKTSDLFTEAGSSLIPLIE
                     SLAPKKEAWFYFPLVDIDSDIHKADIYVTKLNRNAKHIFKCEVAQGSDANIFSSLANN
                     SKDSVFLGYPYGLIEADRLARVTNREKEYYQTILLHKLKGSIDSFLNISSAHSILDSI
                     SF"
     gene            11037..11387
                     /locus_tag="VFF28_00335"
     CDS             11037..11387
                     /locus_tag="VFF28_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44107.1"
                     /translation="MDKRVGNYSFIIGVVIAIVLGIVSLGSVAVWLASVLVILGLVVG
                     FLNVTGKETKDFLYVTAVLVIAAGIGGASSTLGSVQYIGQYLNGVFTQVLAFIVPATV
                     VVALKEIWAIGQQG"
     gene            11419..>11934
                     /locus_tag="VFF28_00340"
     CDS             11419..>11934
                     /locus_tag="VFF28_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HZX44108.1"
                     /translation="MFDVITVGSSTIDVFAKTKVSELIKLKGGDLLAYPVGSKLLIDN
                     LQFTTGGGATNTAVCLARLKNKVACITKTGSGANSQKVINHLKRENIDCSFIVKDKKG
                     RTGYSIILDSIDHDRTILTFKGSNNDLRFSELSKAKLNTKWFFFCTMMGESFNTLKKL
                     AKYAEDNNIKIA"
BASE COUNT         3239 a         2787 c         2200 g         3708 t
ORIGIN      
        1 acctttgaga tgtcagaaac caccactcgc aatctccctg cttcagctcg gagtaagaag
       61 gtggtttctg agcatgctca agaacacgca ccgaggctct tcagcccggc ggttcttgac
      121 aaacaaatca agagttttga aagacagtag agcccccgtc aagagcttaa tacattctaa
      181 tttgtatagg aaagtctatg gcgaagattt actgctttct gttttattgc tttctaactt
      241 ttcagtatgc ttgcatttag gataggttga acaaccaaga aaactcccat agatggattt
      301 gcgaatgacc agtattccgc cgcattttgg gcatttcttc tctccgcttt caacagcttt
      361 tgcttctttg agggcttcac cagtgatgtt tttcgatttg cagtttggat ttaggcaaaa
      421 ttcttttgca gactttttcc tgagctcttt aactattgga aatttgcaga cctcgcattc
      481 ttttttagtt gccttgatat tggcccctct gggtaagctg acagtagtct tgcatgccgg
      541 atattctgag catgctgcaa agcttccgaa ctttcctttc ctgatcatca ggtttccttt
      601 tccgcatgca gggcattttc caagcgtgtt catagtatcc ttggcctgcc agaatgcttg
      661 ttttaagctc tcgccgacga ctttctcatg cttcttgaag tcatttacca ctttcgctat
      721 tgcatccttt gcttctccaa gcactactgc ctgattagtt ttcccttccc ttatctgctc
      781 catctcatct tcaaaatgtc tggtgagctc ttcatcgatt atatttggaa catatttacc
      841 caaggtatct atagtgttta tcccaagctc tgtagcttct atagactgct gtccatgcac
      901 atagctcctc tggaacagag tatcgacgat agaggcgcgt gttgccttgg ttccaagccc
      961 tctcttttcc agttctttta ttatggaagc aggggtgtac ctttttggag gcgatgtttc
     1021 cttgtcatgc atggttattt tcttgatatt caccgtatcg ttttcagtaa ccttaggaag
     1081 ctcttcctct tcaagtttga cataaggccc ataatatatg aaccatcctt tctcgatagt
     1141 ccttgttcct ttggcgacaa atatctcgcc tttgacctca gtgtttgctt tcactgtctc
     1201 acggacagca tcctctccaa acgttgccat gaatcgcctg actataagat catagatctt
     1261 aagggccctg ctctctgcat gaggttcgtt tcctgttgga tatatggcag gatgagcagg
     1321 atcagtcttg cttccattat taggagtaag ctttcctttt aagagcttgc tgcataattc
     1381 agaataatgc ccttggcgtg ataagaggct gaggattttg gaatatccta tcttgggcgt
     1441 cagttggttt gatgaagttc ttggataaga tatgcatcct gaggtataca gctcctgggc
     1501 tatagccagg gtatctttag gctgtatctt aaaaacacga tatgcttcga cctgtagtga
     1561 tgtgagatca aaaggattag gcgcctgctg cttgaattgg cttttctcta cacttgcgat
     1621 ccttccggtt ttctgtccct tgactttttc catcacagag tcagcttctg atttctgcca
     1681 gaatttgtct ttgatatgcc agccggttat ctctcctttg tttacattgg cgttaagttc
     1741 tatctgccag taaggcacag gaataaattt ctttatctct ttctccctat cgactattat
     1801 cttaagtgcc ggtccttgga cccttccaat gctcagcact ttaaagccgc cggtgctttt
     1861 gtaggcagag gtaagcgccc gcgaatagtt tatcccattg aaccaatcaa gaaaatgcct
     1921 tgtctctcct gcattcgcct gtccccagtc aaggctcttt gatttattat catatgcttc
     1981 gacaagatca ggcttggtca atgtagaaaa cttcattcga gaagcatctt tcttatggca
     2041 ggcatatcga acaacattaa gccctatgac ttctccctca acatcataat cagtagcaac
     2101 agtaaattcg tttgcatcct tgcataattt ttttatggcc atcagatact tcttagaaaa
     2161 accagctgac ttttttgact catgtgcagg ctgccattcg atgtcaaaga caggaaactt
     2221 ccagctggat tttttttcat caagcccgta caggtgtcct acagcgcatg ccaccacgat
     2281 atccttcttg ccgcgggtta tcttatagta gggaacaccg ccaagatttt cccttatcgg
     2341 ctttccatca gcaagggcag aggcaatttt ctctgcagct gcaggctttt cagtgattat
     2401 cagctcatac atgattttct cttcagaatc aagacatgtt taaaaaactt tgtgggaaag
     2461 tgtaagaaat ccaatttaca gaaacttaaa tccttagatc aggcggtccg tctatgtggc
     2521 agcggcaggg ctcttttgat aatcttccaa atgcaggtgt gcttggccgg tatgttaacc
     2581 tgccgctaaa gggggttgcg tcataaccag acatagtcac agccattgat cctgcaaaac
     2641 agttaggcat tacaacctgt gcgtttggat tgtcaatgca gccttgcctt gcaattctct
     2701 cttcctgttt tgccgattca atcaaagcta gatactggtg gatatagcaa ctagttatgc
     2761 cgggaacata actgcaaaac tcagctcctc ctggatcaga tcctgctgca ataaatgtta
     2821 ttcctttcct gctgcaataa tgacttatga gcatcctaga gaacatggaa tcagtaaatc
     2881 catagatcac atctatctgt tcaggataat caaaatgttc atcaaatttc ctaacaagcg
     2941 gaacagcaat ctctcctcca accatcttat tatgtttctc tgcgcaaacc tctgctttat
     3001 atcttccaac agaatcccag taaaaaggtt gccttgatag attatggtct ttgacagaat
     3061 catgatccac atatatgact cttgcaggat ttgactcaac aaggattgga actgcaaagc
     3121 atccctggcc gccaactccc acaaccatga ctgtctttcc tgacaactgg tttttatcag
     3181 gcaactttgc atcactagta tgatcaaatg gaaatgtgta tccgggatta agaaacaaag
     3241 gttcatcctc tatctgtttg taaaccataa atcttattgc tttctcaatt gctaatgctc
     3301 ctgcaggcca ggccaaacag caatcctttg gggcaccttt aaactgacca tcagtgtgat
     3361 ttccagtctc tattacgctg tatccttcaa tactttgcat cgaaataaat tgaattcgtc
     3421 gctgcaatgc aaactctaat ccaacctttt ttgaaactgg attatttgtt gcatcaacaa
     3481 tagcttctac accatctagg aaatgattgt cctctctaga ttcaaacgta actggcagcc
     3541 cagataccct aacaaaagga ttaatttttt gcagtacatc agtatattcg gcagaaagat
     3601 ttcttccttt taatgtgaaa tcaagcgcat aactcccttc tacacctatt ccgctaataa
     3661 acagccttgc gtttacaccc acaagagctg catttagggc tgcagggaaa gcaaaaggat
     3721 cctctcctat aattgctatc gttgaatctg caagcttagc ctgcccttcc tgaccccata
     3781 atctttcttg cctgtcatac ctggccattc ctacctccta aaaattgcgg cacgtattga
     3841 tctaacacct tgtttatacc agttattgaa tccgcagaac cgaaatccct aacaaagtcc
     3901 aactccagtt tgtttggcgg agtcaatcgt ttacacacag cctgcgttgc tgagctatat
     3961 tccactaatc ttaacctatc aggttcagga tcctgtgaaa gaggaccaag gctcttaata
     4021 gcgccagata tcccctgctc atcgaggagg cccagcattc cggcaatgta gtgagcatat
     4081 tttctatccg gagctccaaa ttttcccaca tatcctgctg cagtaaaaac aaacctatat
     4141 gcaagttttg cgctatcagg aggttgagat aaaagatatt taccaattcc atgtcctccg
     4201 gttgtgccat atgacctagt ctttttccta tgatgtttgg gatgctcgca aatatgcagg
     4261 ctttttctca tgatttctgc taattctcct tcatcaaacc tcatgtcatt ctcaacagga
     4321 actgttctaa cacctacttc ttttgtacgc cagatgcctt ctgcaggacc gctaaaaagc
     4381 tgggagtagt gatagccaat gattccaaat ggctgtcttc tgctggcatc aaccactaca
     4441 gcctgggcaa tgcctaactg catgatttga cggtgctctg tttccactaa tccaagaagc
     4501 tcgtgacaga aagttaaagg attgaatcct tcacctcttt ttaaaccgtg ctgcttcaat
     4561 aaacctaata ttctctcatc agcaggtata actctgctta ccacgggatc aaattcgctc
     4621 tcactacttg ttatttgcac ccttccgtca attgttacat acggcttagc atcaaaaaga
     4681 gaaaaaagcg ttaatctatt ataaactaac aaatccctcg caattctcct gccttcacta
     4741 ctaaaatcac catagtctgt ccctgaaaag ccctttccaa atcctgcatg gctgtgtaaa
     4801 atcccactta caatataatc tgcgccatcg gcttgcctaa gtgctttaag cccttcatcc
     4861 aaatttgcct ttattgcatg cccgggaacg tatacagaag cagggcctac ataactctca
     4921 gatggaggaa ttatccccct aacaattctc tggttatctc ctcttaaaca aaccatgtcc
     4981 gaatcccact caatatgctg gggtgttcca gtcctttccc ctgcaagtct ccttacgatg
     5041 tcaatagtag taatgcaata atcctgcacc cccttactta ttaaaatctc atccggtata
     5101 agctcttcaa tattcatctt ctttggtaaa cctcaaatcc tcgatttctt gcttgcctga
     5161 ctgtgagttg ccttccttta tttagaaact tgtatgaatt ttcaggagta atggaatgcc
     5221 taagtgtggc tatcccttct gaaatctgat tagcaacatc accaggatag cttccagcat
     5281 tgcatatata atctcccatg tctgcggggt ggtctatttt atcaatcaca aatggtcgtt
     5341 ctcccgtgtc aatctgataa gtatatgaaa ctttcatacc gacccgtgtc tctccaaaga
     5401 agaagcatct tctttcatct ggatcaggat tattatctaa tggatcaact actgcaaatt
     5461 tgcttatcct tctaaacacg caacatgaag ggtatcgtga ctctctataa acaaaaaatc
     5521 ctacaaaatc ccactcaaag tatccgcttt gtattgccct ttcaatatac cactcgggat
     5581 tgcgaattcc attgagtatc tggctatctg ttttaagctg atccagcaat tggtgcctaa
     5641 gttgaagctc aagcatcttt gcatattgcc cgtttagtgt gctaaaatct gcactctcct
     5701 ctaagcaaaa ttctctgtca tcagagctaa tttttggtct gttccatgca aaaaaagaaa
     5761 ttggagaaag cctataaatt tttccactat gaccaaaata gggcatacct ctaaaaattt
     5821 gagctaataa ggaatttccc tgggcaggtg gaaaggatcc tgcaagtgtg ctcttccatg
     5881 ggtcagaata ttgcagctca gaggttatgc attgggtatt tggcagagca gtattttctc
     5941 ttatacggct aacaatctga gggataggta ttccatcaga cggtgcttcc ccgtaactta
     6001 aagcaagtct gtcaaatacc ctgtgaacag cggtcttttg ttccccttca tcaaactgtg
     6061 atctatatgc cccaaattca tacatagcag ggagtatatt ggaaaaaata tgccttacta
     6121 tctcacgcag tttttctgca tcaccaatat tactttgatg ggttgctctg ccaaatccca
     6181 aaagagagtg ttcggacaaa atttcatcca ataaagatgc ccttagtctt tgaatccaag
     6241 aataatgtct gttgcttaaa tcgaatcttt caacctcatc cagtgttcca attggtgtaa
     6301 aaaatacagg accatcactg cttgataatg agtttacacg atcaacaaac ctaatatttg
     6361 ttttggcttt agttggcttg tataagacat tagaagcaat aaaaaaatca gatactaaat
     6421 gatttaatgt gcccccattt agcatcttgt ctcacttttc acctgtattt acctaagact
     6481 ctgccgccga ctttaattgc gtgaagctgg agcgtaccgt caggcaaagt aacactatct
     6541 gtgtgaaaag tgacatagtc accatgtaca gatctgtttt gttggccagg cctacaaagg
     6601 ataaattccc actctctctt tttaccatct cctgtttgtt gttcaagtct tagataagac
     6661 gcaaatctgg tttgctcacg gttaagttct ggtgtatata gtctgtctcc aactgtaact
     6721 tgctcgccca ttgcatatag atgcatctga tataatgttg cgggtggact accaactgcc
     6781 atatctggag ctatttccat ttctcttttt ggtccacttt cacttgttgc ttgaagaatc
     6841 agtaaatatt tctgaggcgc agtagccaac gcaacctcta actgatcagg tttttcacca
     6901 gatcccgctc caactactgc atctatactg cttggtgaag caggaggttg ttcagcaact
     6961 tcttgcatcg gatttgcgtc aggaggggcc cctccttcac cctgagcagt taaatcctca
     7021 ccaatagagg caacaggcgc agtatactga ggattaggtc ctccagtacc atctgcaaac
     7081 ccactaatga ccggtttact tccttttcta accattgtat tcacctctct ctacagattg
     7141 gatagtataa aacatatata tattttatta taccctatcc ctattttgat ttaggcatgt
     7201 aactgactta ccacctatca attcccaatc ttcaaaaatc cggtcagcct ttttaatgtc
     7261 gcgcatgcag acaagacagc catatgcgag gttttgggat tcagcgggct tacaacattt
     7321 tcagtccttg tatatatctt gccaaagctc cctaccactt cgatctcatg gttattggtg
     7381 gaaagagaag ggtcagcgac aatttttacc ttggttttct cagccccaat cccagcaagg
     7441 ctcagtgttg cggcgacgtt gatgttttta ggaaaccccc ttattgcaga aagcgcattt
     7501 ccatcaaaga tgactgtctt tttctttatc ttgtccagct ctatcttgtt ttcaataata
     7561 aagggagcac cagttaggct ctttggattc ttgcttgttg ttatcgtgac agaatctatc
     7621 ctgtcgatgc ttgctgcctt taccccatct ataccacata tggccccaga tgggacaaac
     7681 agccgtgctg ttagcttgtc gagtaattca agattctgta tcactccgcc cacactcatc
     7741 accatcagat gttttttctt tgtaatgcat ttctctagta tgcttctgac aacagaaggg
     7801 tttacagctt ctatgaccag ccatgaatcg ccaatcactt catcgatgga agcaacattt
     7861 ggcttcttct gcaaagactt tgcaagcttc tgtgcttttt cctcatcgat atcatttatc
     7921 gctacgagct catgctgcgg cagctcttta tctataaatt tgcacaacac agaaccgatt
     7981 gcaccgcatc caattactcc gatcttcata taaacctcaa cttcatgtca agcgctttgc
     8041 agcttgtggt aagatatccc atggatatta catcaacacc agtcttggaa taagctttaa
     8101 ggttgtcagg cgtaattccg cccgatgcct ctatcatcac atgattgttt agcgctttta
     8161 atcttctgat tatctttttg atggctgcag gtttcatatt atcgagcatg ataaagcaag
     8221 gtgtattata tgagtttcct tgcagctcct taaatttctg tgcagcctta aatgcatctg
     8281 cttcattaga ggcctctatc tcgataaagg cacctctctt tctattcttc catgcccttt
     8341 ctatagagac aaccacagga tcatgaacgc cttctttctt aagtgcatca aggtgattat
     8401 ccttgataag tatcgactcc caaagagcaa gcctatgcgt aagcccgccc cccacaaaca
     8461 ctgctttttt atctatgaat ctccactcag tctttcttgt aggcgcaatt ccaacctttc
     8521 ctgccttttt caccagttgg ttggtcaaag atgcaatacc gctcatcctt gacagcatat
     8581 caagggcagt cctttcaagc ttgagtatac tgtttgcctt cccgcttatc tccagtatct
     8641 tagagccctt tctaattttt tcaccatcct tcactaactc cctaacatcc aggccatatg
     8701 accttaagat ataacagact tcttctatcc ctgcaataac tcctgcagag cgtgaaaaca
     8761 cagtagcctt tgctttcttg ttcataagca cagcttcaga ggttatatct cctttcccaa
     8821 aatccgcctt tatctcatcg atgataaaag tatcgatcca gtccctgtag aatttactct
     8881 tgactgttaa gagatgccct ctttggaaag acctttcgag tatttttctt ctatccatga
     8941 aaaagatgac taaaagaaac tatttaaatg tttaggatat atttatcatc ctttcaagcg
     9001 ctaactttgc ctttatcatt atttcttcag gcacttccac tttgttcgtc tcattaagca
     9061 ggcagttata taccttgtca agagttatct tcttcatctg tatgcatgtc ccgccagcag
     9121 catagaaagt tttatcgggt ataagccgtt ttaaaacatt agccatcccg cactcagtaa
     9181 cagcgataaa ctcagaagcg ccgctttcct tggcatattt tatcatctgc gctgtcgagc
     9241 atacagcatc agcttcattt atcacttcca tagggcattc aggatgggcc atcaccttgg
     9301 caagtggatg catctccttt gcttttctta cgctttctgc agttatctta tcatgcacat
     9361 agcagtggcc gtcaaaaggg attatcttct tggttgtctt tgtctgggta tatgcagcaa
     9421 ggtttttatc aggcgcaaat attatctcat attcagcaag ggagtttgcc accttcactg
     9481 aatttgcaga tgtgcagcat atgtcagaca cagcttttac agcagcactt gtgttgatat
     9541 agctcatcac agcagcttga gggtgctttt ttttggcctc aagtattttc tcaggcgttg
     9601 ccatcgccgc catcgggcat atcgaaagct tctccggatg aaggaccatc ttttcaggat
     9661 ttaatatttt tgccgattct gccatgaagt caacgccgca gaaaacaatg atctttgctt
     9721 ttgtctccag ggctttcttg gcaagttcaa gagaatcgcc tataaaatcg cttacatcaa
     9781 gtatctcttc cctctggtaa ttatgcccca ggactacggc atttttctct ctctttagct
     9841 catttacctt ttttgcaagc tcattcataa aaaaaagaag ccttcattca tataaaaaac
     9901 tttttaataa ctctccaact ctctttagct tatggatgct atttcagata tccttgattc
     9961 tctaaaagaa ttgaccaaag caaaagggac tatcgtccaa tttaatgacc cttcgtacac
    10021 tccaatcccc atctcaccaa aaaacttcca caagataaga gaaagcgctg gcaaaaagac
    10081 tgcattcatc gatggaggaa atgcagagat tttaaaaagc ccaagtttct cgcttcaatt
    10141 gctaagggtt tgctcggtga tattccagga caacaaaaaa gtatcatcaa ggataaaaga
    10201 atgttttgtc ctgacaaaag caaaggacat tgaaggcaaa atattctatg aaacatcttt
    10261 cttccaagaa gcaagagaaa agataaattt tgactctttt gacggaacga taaggcaagg
    10321 caaccaccgt atatccatct caagcattgc aggtgtcatc cgaaggctgc ttgagcttga
    10381 cctggcagca gaaacaataa tcaacttaga aaaaggagat gctattgtac tggatggaga
    10441 cttaaaggct tcaataaccg gcgaaaagga atatctaaac atattgttgg caaaagcaaa
    10501 ggaaaaaggg gttattgtat cagctctgtc aaagacatcg gaccttttta ctgaggcagg
    10561 aagctccctc atcccactca tcgaaagctt agcgccaaaa aaagaagcgt ggttttattt
    10621 ccctcttgtt gacatagata gcgacatcca taaagcagat atctacgtga caaagctcaa
    10681 caggaatgca aagcatatct tcaaatgcga agtcgcacaa ggctcggatg caaatatttt
    10741 cagctcactc gcaaataatt caaaagactc tgtcttctta ggctatcctt atggattgat
    10801 agaggcagac aggcttgcaa gggtcacaaa cagggaaaaa gaatattatc agactatctt
    10861 gcttcacaag ctaaaaggca gtatagattc atttctaaat ataagcagcg cccactctat
    10921 ccttgattct ataagttttt gacagaaagt tttctcattt gtcaccataa tccttaaata
    10981 ctcatccctc ggtaagtgat atttaaggct aatttgccaa aaaaagaggt gagcgtatgg
    11041 ataagagggt aggaaattac tcttttataa taggtgtggt tatagcaata gtgctgggga
    11101 ttgtttcttt aggctcagta gctgtctggc ttgcatcagt gcttgtgatt cttggcctgg
    11161 tagtaggatt ccttaatgtg accggcaaag agacaaaaga tttcctttat gttacagctg
    11221 tcctggttat agcagcagga atcggtggag caagcagcac tcttggaagc gtgcagtata
    11281 tcggacagta cttgaacggg gttttcaccc aggttctggc tttcatcgtc cctgcaacag
    11341 ttgtagtggc tctaaaagag atttgggcta ttggccagca gggataagtt tttatattcc
    11401 ttttttttaa tttctaaaat gtttgacgta ataacagttg gaagttctac cattgatgtt
    11461 tttgcaaaaa ctaaggtttc agaacttata aagctaaaag gcggcgacct ccttgcctat
    11521 ccagtcggct caaagctcct tatcgacaat cttcagttca ccacaggcgg tggagccaca
    11581 aacacagctg tttgcttggc aaggctaaaa aacaaagttg cctgtataac aaagacaggc
    11641 tcaggtgcaa actcgcagaa agtcataaac catctaaaaa gggaaaacat tgattgttct
    11701 ttcattgtca aagacaagaa aggcaggaca ggctattcta taatacttga cagcatagat
    11761 catgatagga cgatacttac cttcaaaggc tcaaataacg acctaaggtt cagcgaacta
    11821 agcaaagcaa agctaaacac aaaatggttc ttcttctgca ccatgatggg agaatcattt
    11881 aacaccctca agaagcttgc aaaatatgca gaagataaca atataaagat agca
//
LOCUS       DASRUK010000009        13982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_9436, whole genome shotgun sequence.
ACCESSION   DASRUK010000009 DASRUK010000000
VERSION     DASRUK010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 13982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13982
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9436"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VFF28_00345"
     CDS             complement(<1..223)
                     /locus_tag="VFF28_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44109.1"
                     /translation="MQELGLNLVNDSHAVPFDKVNEAVAFMKYGSDLRVMFEMLFLTG
                     CRIKELDKMKISCFYGNVIYWELGKNQGGL"
     gene            complement(415..654)
                     /locus_tag="VFF28_00350"
     CDS             complement(415..654)
                     /locus_tag="VFF28_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44110.1"
                     /translation="MDFAEDLENIRRDFDKLKEDDIFALRNENSNVSAIVFKDGDGTR
                     VRINRGMDRFEQCFIHDNGFIRINERKKPRIEKRI"
     gene            complement(722..1510)
                     /locus_tag="VFF28_00355"
     CDS             complement(722..1510)
                     /locus_tag="VFF28_00355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZX44111.1"
                     /translation="MGPTVSVIMPAFNSSELLVDAIRSVMAQSFSDFEVVLIDHGFRD
                     STVRLCSSFMGDNRFKYHKVEECSLAKARNEGVSRAGGEQVIYLNYDDTFPGDALEVL
                     VKEQQKGFAGTYANMNLMDRSGKVYETRKSRRFECQEDAAWALLSEIRVPILTPTLMA
                     RREVLKECPSDENVGTVEDVEVLLHILQRYPLHYTDHTVHNFRIHNGNYLARTRLDTI
                     GWWNQIYERYVNHSSVKAYLKAKRAGVIIGKYSYETTRNLIWRR"
     gene            1609..3378
                     /locus_tag="VFF28_00360"
     CDS             1609..3378
                     /locus_tag="VFF28_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZX44112.1"
                     /translation="MDTFIVGIGLLFLILVIICYIIKLFHQPIILGYVLSGLAFSVIL
                     ARFSFPNDSLMILSELGVTFLLFLIGLEFSYENFKFMGKDILIATLLQSIIFFVVPFV
                     VSFLFGFSVIESVYLSVLFMFSSTLLVAKWLEDKQDSQTLYGRIVFGILVLQDIIAII
                     FFVILGVLGVENTSKIMVLPIGAIVLLAIAMLFSKYILNRLIKSTLRYPELTFIFGLG
                     VCFFFVEVSILFGFSETIGAFLGGVVLANTIYQNDVSSRLKPLIIFFNMLFFVGLGLK
                     LEFDLPARYFLFMGLFLVMCIIIKPIVVYLTLKGRGFDSKTCAKASVSLAQFSEFGVI
                     IVTTGIASQAILPDMGTISITLLIITMVISSYSIKYNEILSTLMYRRLQFLDKYFLKE
                     KKPTEAVDLSKYDIIVIGYLGLNKELLTKFESAGSKILVIENDPVNVQLLRNAGINFI
                     YNSVTNLYFFEHLKFERIKLLISNLVDVQDNIMILKKLKEENPLSHSILSAKNLKDSL
                     MLYDAGADYVICSTEIKENQVSMLLQDYSQDINKLIEKKIPEITRLKEKKNTREAVQY
                     YEWDDFVKKMFRRNKGLEGTENL"
     gene            3415..4371
                     /locus_tag="VFF28_00365"
     CDS             3415..4371
                     /locus_tag="VFF28_00365"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZX44113.1"
                     /translation="MENPKYAFLFIILLGVVAFMLIREFITSILSAFVLSYLLLPLHK
                     LMSRKLPRKLSAIIAVLILVAVLFLVIYYFTSTAVAQAQAYLTQETIEGIFSTFLSWI
                     PEIPVANYIGSISSVIRGYVVGLSLSIISQIPHITLSILVTLFLTYYLILNWQKTTDF
                     LVKLIPIRNKKEVAAKVGLSARKIVYGYTIIAAFDFIIALIGFKVAGIELAVFLALLI
                     GVSAFIPFFGPASVWLPVFLFNILRQNYFSAITILITGLIISVFVDNLLSYVLVGRQS
                     DINPAFMLLGVFGGVAYFGILGFIIGPLIIVIFIDFLAEFIR"
     gene            complement(4324..4500)
                     /locus_tag="VFF28_00370"
     CDS             complement(4324..4500)
                     /locus_tag="VFF28_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44114.1"
                     /translation="MRTKVCPYCREIFYDLDFPAQFERMVTCGKVGCMRLRRWERLGL
                     SDKLGKKVDEDNYY"
     gene            complement(4573..4710)
                     /locus_tag="VFF28_00375"
     CDS             complement(4573..4710)
                     /locus_tag="VFF28_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44115.1"
                     /translation="MIESMPGDEPIIMESVPRLIYCGGCMVEMTMQGKHLACPLCHLH
                     Y"
     gene            4828..5832
                     /locus_tag="VFF28_00380"
     CDS             4828..5832
                     /locus_tag="VFF28_00380"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HZX44116.1"
                     /translation="MDARKIISFGKNSSIVSLPKQWLIKHKLKKGDILNVYESKDGLM
                     LRASNSAIKDEPKTACISADHKSLFRLKTEIVSAYLDNFNIIEVYSPELKKAAVEIKK
                     IIHDLSGMEIINQTSTRIAAKDLMNISEISVETLIRRMDNIARSMMKDCLDCFKGNDQ
                     SDSLSQMDEEINRLCYLANRVIRGGLKDVRVANSLGVDNLKLHTDHTVTIKVESIADN
                     VKRISRALKESKLNETWRVRLREIFLDIEKGYLDVMKAYYTNDQSIALQIEEQNSKRL
                     LSCDKFFGGHSHKELKIELKQGICEYRNACGATTRIIEDMKTMCSGIKHIARTIIGGG
                     "
     gene            6833..7312
                     /locus_tag="VFF28_00385"
     CDS             6833..7312
                     /locus_tag="VFF28_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44117.1"
                     /translation="MELERKIKLLKKSYMFGYAAEAAWINWVFPNFQENFDQINLEHE
                     LPNVYKNFENIQRHYDKVVPFYEISREMEARDWDDFAVLFRVRDFLPKFREFLDDYVY
                     FLRDNNYYAPRGSEIDKRREDIWAEWFDAESYQGMTLKDRMMKYDADFLQKPAPSQK"
     gene            complement(7417..8523)
                     /locus_tag="VFF28_00390"
     CDS             complement(7417..8523)
                     /locus_tag="VFF28_00390"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HZX44118.1"
                     /translation="MDKADYQGRESKAVSAIKKAIPKKLKANMDPISDGMFAELYHND
                     EVFPNHLMACAIDGVGTKLILAEAMEKYDTVGIDLVAMSSNDLATLGKVAPFLFIDYF
                     AVQKEFQEKALTGPLMEGIVKGLEMADAGDILRSSIHMNIGKGETASVDELISGLNPG
                     MGFDIAGGMIGFIRKEDINKSIKVGDKIIALKSSGPHSNGYTDLRLKLLDGDFDQRLE
                     HKKKYTGKHKLDDRFEDSTIGETLLTPTRIYNKVVADMASKAKIMGMNNTGYGLKNLN
                     RIKGFQFEINNPITPHAIFQLMQKDSGFSDEEMYKRFNMGMGFFVIADKDDADGLISI
                     ADKHKEAAQVVGEVKKGSGTVLVKGSKRILFEGY"
     gene            8631..9071
                     /locus_tag="VFF28_00395"
     CDS             8631..9071
                     /locus_tag="VFF28_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44119.1"
                     /translation="MFSMVFYGKHTPYERIAFLSIRDTQKPVDYSAQIEAELSKLEGP
                     FAQDLKDLYSLCQITSLGLSNPHGVNSIDERADEDGLGIPDHAYVADNDPSNPTLPFV
                     FSQAMDNVASNMVGILKRVEGKSLPPALEYMVNKARQYIQRLEY"
     gene            complement(9090..10745)
                     /locus_tag="VFF28_00400"
     CDS             complement(9090..10745)
                     /locus_tag="VFF28_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HZX44120.1"
                     /translation="MELEKIFTKKNIEILNLLEKESLHIRDIADRLKISPAKVHGTIQ
                     LFKHHNIVNETSDKNRKVIKLNKNSALLKNIESLMDTEKKEETEEIMPLFDNISPLDY
                     RYYGRSEKIKDKLKPYLSEEASVKYLAKVEAALTKVLAKRKLCSKEIAIEVENASRQV
                     TAQEVYMEEDRIKHNVRALANSIRKRVSKKAKPYVHFTTTSHDIICTADAARYKDFSS
                     DVLVPALINLERTLIFLALREKNTLQIGRTHGQHAEPITFGFALAQYVSRLGSRIKQV
                     QKTSANLRGKIAGAVGAYNASSLFFTDPEIFEKEVLEELNLKASPISTQVVEAEYITD
                     FVHSVISCFGVLANLSDDMRNLQRSEIAEIAEAFEEKQVGSSTMPHKRNPINFENVKS
                     MWKEFMPRMDTIYMDQICEHQRDLTNSATSRFIPEILAGLYVSATRLNRTMSKLIVDK
                     ANLKRNFDQNKEMITAEPAYILLAAHNHPDAHEHVRELTLKSQQTGKPFRELFFEDET
                     LMPYITKFTKRQIEILKNPEKYTGIAGSKVESLCNFWKKELKI"
     gene            complement(10860..11090)
                     /locus_tag="VFF28_00405"
     CDS             complement(10860..11090)
                     /locus_tag="VFF28_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44121.1"
                     /translation="MGKVIAKTGIKREEGYLYFVNKAGDVARVQMARRGEKTSKKQEV
                     VAKAGVDRKTGFLYYVDKQGNVCEAKMARGRK"
     gene            complement(11239..12012)
                     /locus_tag="VFF28_00410"
     CDS             complement(11239..12012)
                     /locus_tag="VFF28_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HZX44122.1"
                     /translation="MPATFRETAIKAAKKAGAIQERYYERSYSIKEKKDKSFATEADV
                     QCSKAIKKIISSAFPDHDIIDEESKPTHKESDYRWIIDPIDGTHNFMVGSPLFGASIA
                     LQYRKEVILGVIYFPILKKLYIAEKGKGVSCNGKKISVNKNQDLKQSFFMFDSHIKRD
                     TDLKLKLLKKIAARTWSVRLTGVCVFNLASIANGGIEFYIDFGIHSWDISAGALILEE
                     AGGKVTDLEGNRWTPEIKDFVASNGAIHNRVLKLIKNSR"
     gene            complement(11996..12961)
                     /locus_tag="VFF28_00415"
     CDS             complement(11996..12961)
                     /locus_tag="VFF28_00415"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HZX44123.1"
                     /translation="MLAEDIKEQVKFTLEETDLEGLGKKKKGKVRDVYTTEDKVFLIS
                     TDRQSAFDRILAKIPFKGQVLTQTSAFWFEHTKDIIENHIIDVPDPNVVVAKKLNVFP
                     VEVVVRGYITGVTDTAAWTSYSKGERNFCGNILPEGLKKNQKFARPIITPTTKSEEHD
                     EKISGKEIVEKELMTMDQWDYVSKKALELFKRGTEIAEKNGLILVDTKYEFGCDDLGN
                     IYLIDEIHTPDSSRFWIKQTYEERFKKGQEPENIDKEFLRLWFVEHSDPYHDEVLPPA
                     PEELIVELSRRYMILYEMITGLTFKAEVGDVRERIERNLKDAGYI"
     gene            complement(12965..13822)
                     /locus_tag="VFF28_00420"
     CDS             complement(12965..13822)
                     /locus_tag="VFF28_00420"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HZX44124.1"
                     /translation="MPKVLVIFGSKSDEQIYNAIVKGLKKEKVDVDLRISSAHRTPEE
                     LNRIINADFDLIIAGAGLSAALPGVIASKTITPVIGVPCKGSYQGLDALLSIMQMPAG
                     IPVLSVGISGAQTAVKNVIEILKPHYSVNLINEMPSDAMSKAEKIFTEFDIPVQRSSS
                     IDTRSINIEFVTLDEPVPEKDALVIYCPILMGEDDKAEAALNILKHSNHGLWVGLNNG
                     KNAALAAIEILNKGGKYTLKLNEFRAKQKEEVKKSDAEVMKSEGSHRAKALASSDFST
                     SKSPVGSVR"
BASE COUNT         3722 a         2979 c         2722 g         4559 t
ORIGIN      
        1 ttaagcctcc ctggttcttt ccgagctccc aatagatcac atttccatag aaacagctta
       61 tcttcatctt gtcaagctct tttattcggc agccagtaag aaaaagcatc tcaaacatta
      121 ctcttaggtc tgagccatac ttcataaatg ctacagcctc atttaccttg tcaaaaggta
      181 ctgcatggct gtcattaact aggtttaacc ccaattcctg cataacataa accccctgct
      241 tccctagtct ataaatcact aaggaaggtt tgtatttaaa tcttgttgtt accgtctaat
      301 ggtttcaaaa ctacacaaag cctagaataa gctaaattta attgtctggc cagactcata
      361 gccttggctt accacccctc gatgtatgaa ttaaaaaagt aattactttg ctttttatat
      421 cctcttctca atccttggct tctttctttc attgattctg ataaatccat tgtcgtggat
      481 gaagcactgc tcaaatctat ccatccccct gtttatcctg acccttgtgc catcgccatc
      541 cttgaagacg atggctgata cattgctgtt ttcatttctc aaggcaaata tatcatcctc
      601 cttaagcttg tcaaagtccc ttctaatgtt ttccaggtcc tcagcaaagt ccacatcaga
      661 aatgattata gggatttcgt gatgggattc aatcccatgt agggcatttt tttgaatctt
      721 tctatctacg ccaaatcaaa ttccgggtag tttcataact atatttacca attatcactc
      781 ccgctctctt tgcttttaga taagccttga cagaagagtg attgacatac ctttcatata
      841 tctgattcca ccatcctatc gtgtccaacc ttgtccgagc cagataattg ccgttgtgaa
      901 tcctaaagtt atggactgtg tggtctgtat aatgtaaggg gtatctctgc agaatatgca
      961 atagcacttc cacatcttca acggttccta cgttttcatc agaggggcat tccttcaata
     1021 cctctctcct tgccatcaat gttggggtca atatggggac gcgtatctct gaaagtaatg
     1081 cccaagcggc atcttcttgg cattcaaatc ttcgtgattt tctggtttca tataccttgc
     1141 ctgaccgatc cataagattc atattcgcat aagttccggc aaatcctttc tgctgttctt
     1201 ttaccagaac ttcaagggca tctcctggaa atgtatcgtc atagttaaga tagattacct
     1261 gctctcctcc agctcgggat actccttcat ttcttgcttt ggccagggag cattcttcca
     1321 ccttatgata cttaaaccta ttatccccca taaaactgga gcataaccta acggtgctat
     1381 ccctaaatcc atggtcgatt agaacaacct caaaatcgct aaaactttga gccataacgc
     1441 ttctgatagc atctacaagc agctctgatg aattaaaagc aggcattatc actgatactg
     1501 taggacccat atcttgagga ttttatatat gcttaaaaag atatgcccta ctttcagcaa
     1561 actataaata caactgcgac caatcaatct ctatcttagt gattaattat ggacacgttt
     1621 atcgttggca tcgggctttt gtttcttatc cttgtcatca tatgctacat aatcaagctc
     1681 ttccatcagc ccatcatcct tggatatgtg ctgtcaggcc tggctttttc ggtgatactt
     1741 gcgaggtttt cctttccaaa cgacagcctg atgatactat cagagctggg ggtcaccttt
     1801 ctcctctttc ttatcggact ggagttcagc tatgaaaact tcaagtttat gggcaaagac
     1861 atcctcatag cgacgctgct ccaatccata atattctttg ttgtcccttt tgtcgtcagt
     1921 ttcctttttg gattttcggt catcgagtca gtgtatctgt ctgtgctttt catgttctca
     1981 agcaccttgc ttgtcgccaa atggcttgag gacaagcagg attcacagac actctatggc
     2041 aggatagtct ttggcatact tgtgctgcaa gacataatcg caataatctt ttttgtcatc
     2101 cttggagtcc ttggcgtcga aaatacctca aagataatgg tgcttccgat aggggctata
     2161 gtccttcttg ctattgcaat gcttttttca aagtatatat taaacaggct tatcaagtca
     2221 accctgagat atccagaact gacttttatc ttcggtcttg gcgtatgctt cttttttgtg
     2281 gaagtttcta tactttttgg attttctgag acaataggtg cttttcttgg aggtgttgtc
     2341 cttgccaaca ctatatatca aaacgatgtc tcaagcaggc ttaaacccct gataatcttc
     2401 tttaacatgc tgttttttgt cggtcttggc ctaaagcttg agtttgatct gccggcaaga
     2461 tatttcttgt ttatgggcct tttccttgtg atgtgcatta taatcaagcc tatagtagtc
     2521 tatctaacat taaaaggaag aggctttgac tcaaagacat gcgccaaggc gtctgtatct
     2581 cttgcgcagt tcagcgaatt tggagtcatc atcgtcacta cgggaattgc ttcgcaagca
     2641 atacttcctg acatgggcac tatctccatc acattgctga taatcacaat ggtcatatct
     2701 tcatattcta taaaatataa cgaaattctt tcaactttga tgtacagaag gctccaattc
     2761 ctagacaaat attttttgaa agaaaagaag ccgacagagg ctgtagacct tagcaagtat
     2821 gatatcatag tcatcggcta tcttgggcta aacaaggaac tccttacgaa atttgaaagc
     2881 gcaggaagca agatactcgt catagaaaac gatcctgtaa atgtccagct gctgaggaat
     2941 gcagggataa acttcatcta caactcagtc accaacctgt atttctttga gcaccttaag
     3001 ttcgagagga taaaactgct gatatccaac cttgtcgatg tccaggataa tattatgata
     3061 ctcaagaaac taaaagagga aaaccccctc tcacacagca tattatctgc aaaaaacctg
     3121 aaagactccc ttatgcttta tgatgcaggg gctgattatg tcatctgctc gacagagata
     3181 aaagaaaacc aggtctccat gctcctgcag gactattccc aggacatcaa caagctaatc
     3241 gagaaaaaga tccctgagat aacccggctt aaggaaaaga aaaatacacg ggaagctgtg
     3301 caatactatg agtgggatga ttttgtcaag aagatgttca gaaggaacaa agggcttgag
     3361 gggacagaga acctttagca aatcttaata taagcaaatc ccttgtctga acggatggaa
     3421 aatccaaagt atgcgtttct tttcataata ttgctgggtg tagttgcctt catgcttata
     3481 agggagttta ttacctcaat cctatctgct tttgtcctta gctatcttct gcttcctttg
     3541 cataagctta tgtcaaggaa gctgccaaga aagctttcag caatcatagc tgtgcttatc
     3601 cttgtcgctg tgcttttttt agtgatctat tattttacat caactgctgt ggcgcaagca
     3661 caggcatacc ttactcagga aacgatagag ggcatcttta gcacattttt gtcatggata
     3721 cctgaaatcc ctgtggcaaa ctacataggg tcaatatctt ctgttatcag aggatatgtt
     3781 gtaggattat cgctgtcaat catatcccag atcccccaca taacgctaag catccttgtc
     3841 acacttttct taacttatta tctcatatta aactggcaga aaacaacaga ctttcttgtc
     3901 aagctcatcc cgattaggaa caagaaagag gtagcagcaa aagtcggctt atctgcaagg
     3961 aagatagtgt atggatatac tatcatcgcc gcctttgatt ttatcatagc gcttatagga
     4021 ttcaaggtag caggcataga gcttgcagtc tttctggcac ttctgatagg ggtatctgcg
     4081 ttcatccctt tctttgggcc tgcttctgtc tggcttcctg ttttcctctt taatatccta
     4141 aggcagaatt atttttctgc aataaccata cttatcacag ggcttataat aagtgtcttt
     4201 gtagataatc ttctgtccta tgtgcttgtc ggcaggcagt ctgacataaa ccctgcattc
     4261 atgctgcttg gagtctttgg aggagttgcc tattttggca tccttggctt tatcatcggc
     4321 ccgctaataa tagttatctt catcgacttt cttgccgagt ttatcagata ggcccagcct
     4381 ttcccatctt ctaagccgca tgcatcctac ttttccgcaa gtcaccatcc tttcaaactg
     4441 ggcaggaaaa tccaggtcat agaagatctc cctgcaatat gggcatactt tggtcctcat
     4501 ggttccctcc ttttttcctt gaattcttcc atcctctcat tgtatgattc tatctgcttc
     4561 atattttcac ctttagtaat gaagatggca caaagggcag gcaaggtgtt ttccctgcat
     4621 tgtcatctct accatgcatc ctccgcaata aatcagcctt ggcacgctct ccattattat
     4681 cggctcatcc ccaggcatac tctcaatcat ttttccccac acccatatat gctagaaagt
     4741 tttatatata acaatcctat atggtatata tagcatatat agggtatata gcaagggtta
     4801 ttatacactc aaaagcaggg gtgctttatg gacgcaagaa agataatcag ctttgggaaa
     4861 aattcaagca tcgtctctct tcctaaacag tggcttatca agcataaact caaaaaaggg
     4921 gatatcttga acgtctatga aagtaaagac gggctcatgc ttagagcaag caactcggct
     4981 ataaaggacg agccaaagac tgcctgcatc tctgctgacc ataaaagctt gtttaggctc
     5041 aagacagaga tcgtctctgc ttaccttgac aacttcaata taatagaggt ttattctcct
     5101 gaactcaaga aagctgctgt tgagataaaa aagataatcc atgacctttc agggatggaa
     5161 ataataaacc agacctctac ccgcatcgct gcaaaggacc tgatgaatat cagcgagata
     5221 tctgtagaga cccttatcag gaggatggac aacatagcca ggtctatgat gaaagattgc
     5281 ctggattgct ttaagggcaa tgatcagtca gacagcctga gccagatgga tgaggaaata
     5341 aacaggttat gctaccttgc aaacagggta atccgaggcg ggcttaagga tgtaagggtt
     5401 gcaaattcgc ttggtgtcga caatcttaaa ctccatacag accatactgt aactatcaaa
     5461 gtagagtcta tcgctgacaa tgtaaaaagg ataagcaggg ctttgaagga atccaagctc
     5521 aatgagacct ggagggttag gctaagagag atattcctcg acattgaaaa aggatatctt
     5581 gatgtgatga aggcatacta tactaacgac cagtcgatag cgcttcaaat tgaggagcaa
     5641 aacagcaagc gactgctttc ttgcgacaaa ttctttggag ggcacagcca taaggaactt
     5701 aagatagagc taaagcaggg gatttgtgag tataggaatg catgcggagc gacaacaaga
     5761 ataattgaag atatgaagac aatgtgttct gggataaagc atatagctag gacgataatc
     5821 gggggagggt aaatgtttta tatacaactc cagattcatg gccttatttt catgggagca
     5881 gctaagtgtt aatcatatcc ttaatcacat tagtcatttc agtaacagat agcgctgatt
     5941 tcaagattgt tttattatca atctgttcaa tagtgatggt agtttcagga ttcttaattg
     6001 aacaaagcaa gtttggcatt tttcaaaccc accgagcgag ttattaaacg aagtttaaaa
     6061 cgagggcaga gctggggctc gagctggaaa tttgggattt gggagagcag gagaaaactc
     6121 ttggagtttt agcaaagcga ccctgcgaac cgttaaacgg cacgttaaac tctccgccag
     6181 ttacgtaagt tctggcggac tagcgaccga aaagtcagaa atcggtgctt cacagaacga
     6241 agtgatactt agtgtaaaag gagtaagggc aaaaggttaa gatagtctgt ttaattctgc
     6301 ttcaagatcg ccttgctgtt caggcaattt atggtttcct acagcggtaa ctttgtaatt
     6361 gtccgccata ttggatgaat caaggctatg attataaaca ccaccagtgc atctaacctt
     6421 gtaagctatt tctcttccta ggagaagatt tgcagtaata cccatagggg attcatttct
     6481 atcaatgcca tttattctga gccatcacag cttaacatac gttccaatac ttttaacgcg
     6541 tccgaaatag ggattagatg gtttgcgtga agttttatgc tgtttacttg attcataata
     6601 ttaaggaatc tttgatctgt tttaaacctt ttgccccttc tgccctagag caccgatttc
     6661 tgattgagtt taacatcgcg atcgctgtta tatccttttc ccctacgtag tgggggaaaa
     6721 ttgcgtccgc aaagtcgaga atgagtccgt cataaagctc gaagagcaat caaatattaa
     6781 gtcaagggct ggcttacata tatttttaaa ctaatatata ttctcgagga ttatggaatt
     6841 agagagaaaa ataaagttat tgaagaaatc atatatgttt ggatatgctg cagaagctgc
     6901 ttggataaat tgggtcttcc caaactttca ggagaatttc gatcaaataa acctagaaca
     6961 tgaacttcca aatgtttata aaaattttga gaatatccaa aggcattatg ataaggttgt
     7021 tccattttat gaaatctcaa gagagatgga agctcgggat tgggatgatt ttgcagtttt
     7081 attccgagtt agagatttct taccaaaatt ccgagaattt cttgacgatt atgtttattt
     7141 tttaagagat aataattatt atgctccgcg tggaagtgag atagataaaa gacgtgaaga
     7201 tatttgggca gaatggtttg atgctgagag ttatcaggga atgactctta aggatagaat
     7261 gatgaagtat gacgctgatt ttctacaaaa gccagcccca tcacaaaaat aggactcatt
     7321 ctcgattgga gataacaaga attaactgca tttttgcaca gacgaaagat gatcgataca
     7381 tgtcaacggg caggcaattc tcatctacag taggcattaa tacccttcaa acagaattct
     7441 cttgctgcct ttgactaaaa ctgtgcctga gcctttcttt acctcgccga caacctgtgc
     7501 tgcctctttg tgcttatctg caatcgagat aaggccatca gcatcgtctt tatccgctat
     7561 cacaaagaat cccataccca tgttaaagcg cttgtacatc tcttcgtcag agaagccaga
     7621 atctttttgc atcagttgga aaatagcatg gggcgttatt ggattgttta tctcaaactg
     7681 gaagcctttt atcctgttca ggttttttaa gccataaccg gtattgttca tgcccattat
     7741 tttggcttta gatgccatgt ctgcgacaac cttattgtag atccttgtcg gtgtgagaag
     7801 tgtttctccg atggttgaat cttcgaatct gtcatctagc ttgtgctttc ctgtgtattt
     7861 tttcttatgc tctaaccttt ggtcaaaatc tccatctaac aacttcagcc ttaagtcggt
     7921 gtaaccattt gagtgagggc ctgaactttt tagcgcaata atcttgtctc caacctttat
     7981 tgatttgttg atatcttcct tccttatgaa gccgatcatc ccaccagcga tatcaaatcc
     8041 catgccaggg tttaatcctg aaataagctc gtcaactgat gcagtctctc ctttgcctat
     8101 gttcatatgg atggatgacc ttaggatgtc tccagcatct gccatctcga ggccttttac
     8161 tatcccttcc attagagggc ctgtcaaggc tttttcctgg aattcttttt gaacggcaaa
     8221 ataatctatg aagaggaaag gcgcaacttt tccaagtgtg gcaaggtcgt ttgatgacat
     8281 tgctacaagg tcaattccaa ctgtatcata cttttccatt gcttctgcca atatgagctt
     8341 tgttcccaca ccgtcaatgg cacaagccat caaatggtta gggaagactt catcgttatg
     8401 atacaactca gcaaacatgc catcacttat agggtccata tttgccttaa gtttctttgg
     8461 gatagctttt tttatcgcag aaacagcttt tgattccctg ccttggtagt ctgctttgtc
     8521 catcgataat ccaaataaat caaggatata taaagtttta ggattgagtt gtattgttac
     8581 tgtttggtgg ttaaataata gaaaggttta tatataatat ctgctaattg atgtttagta
     8641 tggtatttta tggaaagcac actccctatg agcgtatagc cttccttagc attagggata
     8701 cacaaaaacc agtagactac tcggcacaga tagaagcaga attgagtaag ttggaaggac
     8761 cttttgcgca ggatttgaaa gacctttatt cattgtgcca gataacaagc cttggcctat
     8821 caaaccctca tggagtgaac tctatcgatg aaagagcaga tgaagatggg cttggtattc
     8881 ctgatcatgc atatgtagca gataatgacc cctcaaatcc taccctacca tttgtttttt
     8941 ctcaggcgat ggacaacgtc gcatcaaata tggtgggaat actgaaacgt gttgaaggta
     9001 agtcattgcc gcctgcttta gaatatatgg ttaataaagc cagacaatat attcaaaggc
     9061 tggaatatta ataccgttcc tcgacaagct catatcttca actccttctt ccagaaattg
     9121 cacaagcttt cgactttgct tcctgctatc ccggtatact tctcaggatt tttcaatatc
     9181 tcaatctgtc tctttgtgaa ctttgtgata taaggcatca atgtttcatc ttcaaagaat
     9241 aattccctaa atggctttcc ggtttgctgg gatttcaaag taagctctct tacatgctca
     9301 tgggcatctg gatggttgtg agctgcaaga aggatgtagg caggctctgc tgtaatcatc
     9361 tctttgtttt ggtcaaaatt cctcttaaga ttagctttgt ctacgatgag ctttgacatt
     9421 gttctattca gccttgtcgc tgaaacgtat aggcctgcca atatctctgg gataaacctt
     9481 gaggttgccg aattagtgag gtctctttga tgctcacata tctggtccat atagatggta
     9541 tccatccttg gcatgaactc tttccacata gacttgacgt tttcaaagtt tatcggattt
     9601 ctcttgtgag gcatcgtcga tgagcctacc tgtttttctt caaaggcttc tgctatctct
     9661 gctatctcgg agcgctgcaa gttcctcata tcgtctgaga ggtttgctag cactccaaag
     9721 cagcttatca ctgaatggac aaagtcagtt atgtattctg cttctacgac ctgggtggag
     9781 ataggtgatg cttttaggtt tagctcttcc aagacttcct tttcgaagat ttcagggtct
     9841 gtgaaaaata aggaagatgc gttgtaggct ccaacagctc ctgcaatttt tcctctaagg
     9901 tttgctgagg ttttttgcac ttgctttatc ctgctgccaa gccttgaaac gtactgagca
     9961 agggcaaatc caaacgtgat tggctctgca tgctggccgt gcgtccttcc tatctgcaag
    10021 gtgtttttct ctcttaaggc aaggaatatc aacgtccttt ccaggtttat caatgccggg
    10081 accaaaacat cacttgaaaa atctttgtat cttgctgcgt ctgctgtaca gattatatca
    10141 tgggaggtcg tcgtaaaatg gacatagggc tttgcttttt tggacactct ttttcttatg
    10201 gagtttgcta gtgcccggac attgtgtttt atcctgtcct cctccatata tacttcctgt
    10261 gctgtaactt gccttgacgc attttcgacc tcaattgcta tctcttttga gcaaagcttt
    10321 cttttggcaa gaacttttgt cagggctgcc tcgacctttg ccaaatattt tacagacgct
    10381 tcttcgctta gatagggctt tagcttatct tttatcttct cactcctgcc gtaataccta
    10441 taatcaagcg ggctaatatt gtcaaacaaa ggcattatct cttctgtctc ttcctttttt
    10501 tctgtgtcca tgagactttc tatgtttttt aatagagcgc tatttttatt tagctttatg
    10561 acctttcggt tcttgtcgct tgtttcgttt acaatattat ggtgcttgaa tagctggata
    10621 gtgccatgaa cttttgcagg gcttatctta agcctgtcgg caatatctct tatgtgcaac
    10681 gattcttttt ctaaaaggtt taagatctcg atgttcttct tcgtgaatat cttttctagt
    10741 tccatattta ccaccttcag aatgaataaa atgttcagta tttataaact ttattgttaa
    10801 tcaatcaatg atttgttcgt ttaataaaaa cagaaataat aaagaaaaag aaataaggtc
    10861 tactttcttc cccttgccat cttggcttcg cagacgttgc cttgcttgtc gacgtagtac
    10921 aagaagcctg tttttctgtc gacgcctgct tttgcgacga cttcctgctt cttgcttgtc
    10981 ttttctcctc ttcttgccat ctgcactctt gcgacgtctc ctgctttgtt tacaaagtag
    11041 agatatcctt cttctctttt tattccggtt tttgcgataa cttttcccat taaataccac
    11101 ctcgttaggc gattttataa tgaggttatt taaattactt tcgggttttg gagacataaa
    11161 aaaatatttt atagaattat ttataaataa aatgatgatt ttttgtgggc tttttagaat
    11221 atctacaaaa tcagcagctt aacggctgtt ttttataagc tttaacaccc tgttgtgtat
    11281 cgcaccgtta gaggcaacaa aatccttaat ctcaggtgtc catctattgc cttctaaatc
    11341 agtaactttt ccacctgctt cttctaagat aagggctccg gcagagatat cccatgagtg
    11401 gatgccaaaa tctatgtaaa attcaatgcc cccatttgct atagaggcaa gattaaagac
    11461 gcatacgccg gtcaacctaa cagaccatgt tcgtgctgca atctttttta gcagcttgag
    11521 ttttaggtct gtgtctcttt ttatatggct gtcaaacatg aaaaatgact gttttaagtc
    11581 ttggttttta ttgacagaga tttttttgcc attgcatgaa actcccttgc ctttttcagc
    11641 tatgtataac tttttcagga ttggaaaata gatgaccccc agaatcactt cttttctgta
    11701 ttgaagtgca atagatgctc caaacaaggg ggagccgacc ataaaattat gagttccatc
    11761 tataggatcg attatccacc tgtagtctga ctctttgtga gtgggctttg actcttcatc
    11821 gatgatatcg tggtccggaa atgcgcttga gataatcttc ttaatagctt ttgaacactg
    11881 aacgtctgct tctgttgcaa agctcttgtc ctttttttcc tttatagaat aggatctctc
    11941 ataatacctt tcttggatag cccctgcctt ttttgctgcc tttatcgcag tctctctaaa
    12001 tgtagccggc atccttaagg ttcctctcaa tgcgctccct tacgtcgcct acttctgctt
    12061 taaatgtaag gcctgttatc atctcgtaaa ggatcatata ccttctggaa agttcgacga
    12121 taagctcttc tggcgccgga ggaaggactt cgtcatgata tgggtcagaa tgctcgacaa
    12181 accacagcct taggaattcc ttgtcaatgt tttctggctc ctgtcctttc ttgaagcgct
    12241 cttcataggt ctgctttatc caaaaccttg aagaatcagg ggtatgtatc tcgtcgatga
    12301 gatagatatt gccgaggtcg tcgcagccaa actcgtattt tgtgtcaaca aggatcaggc
    12361 cgtttttttc tgcaatctct gtgccccttt tgaaaagctc aagggctttt tttgagacgt
    12421 aatcccattg atccattgtc atcaactctt tttccacgat ctcttttcca gatatctttt
    12481 catcatgctc ttctgatttt gtagtaggag ttattatagg ccttgcaaac ttttgattct
    12541 tttttaggcc ttcaggaagg atattgccgc agaaattcct ttcgcctttt gagtaagagg
    12601 tccatgctgc tgtgtctgta actcctgtga tatatcccct tacaacaacc tcaacaggga
    12661 aaacatttag cttctttgcc acgacaacgt ttgggtcagg gacatcgatt atatggtttt
    12721 caatgatatc cttcgtgtgc tcaaaccaga atgctgatgt ttgagtaagg acctggcctt
    12781 taaagggtat ctttgcaagg atcctgtcaa atgctgactg cctgtctgtc gagatgagaa
    12841 ataccttgtc ttctgttgta tagacatccc tgacttttcc tttcttcttt tttccaaggc
    12901 cttcaagatc agtctcttca agcgtaaatt taacttgctc ttttatatct tcagccagca
    12961 ttttttacct cacagagcca acagggctct ttgaggtgct aaaatccgag ctagccaggg
    13021 ctttagcccg atgagaaccc tcggatttca tcacctctgc atcgcttttt ttcacttctt
    13081 ctttttgttt tgccctaaac tcgtttaact ttaatgtgta tttgccacct ttatttagga
    13141 tctcgattgc agcaagggct gcatttttgc cattgttgag gcccacccat aggccatgat
    13201 tgctatgctt aagaatgttt agtgcagctt ctgccttatc atcttcgccc ataaggatag
    13261 ggcagtatat gaccaaggcg tctttttctg gcacaggctc atcaagtgtg acaaattcaa
    13321 tgtttatcga tctagtgtct atggagcttg atctttggac agggatgtca aactcggtga
    13381 aaatcttttc tgcctttgac attgcatcac ttggcatctc atttatgagg tttacactgt
    13441 agtggggctt tagaatctca atcacgtttt ttacagctgt ctgtgctcct gaaatcccta
    13501 cgcttagcac aggaattcct gcaggcatct gcattatcga caacagagca tcaagccctt
    13561 ggtagcttcc tttgcaggga acgccgatga ccggtgttat cgtctttgaa gcgataactc
    13621 ctgggagtgc tgctgaaagg cctgcacctg caataatgag atcaaagtct gcatttatta
    13681 tgcggtttag ctcttctgga gtcctgtggg ctgagcttat ccttaagtca acatctacct
    13741 tttctttttt tagtcccttg actatagcgt tgtagatctg ctcatctgat ttagagccga
    13801 aaataaccag gacttttggc atacgattca cccttcttaa gaattaggct gatttgttta
    13861 aatatttttt gatgctagtg gctttgcgca caaattactt tgggcgtgag ttatagaact
    13921 tgaatacttc gattacatgg tcaacattgc caagatacat caataccttt tgttttacct
    13981 tg
//
LOCUS       DASRUK010000010         2203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_842112, whole genome shotgun sequence.
ACCESSION   DASRUK010000010 DASRUK010000000
VERSION     DASRUK010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842112"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..571
                     /locus_tag="VFF28_00425"
     CDS             227..571
                     /locus_tag="VFF28_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HZX44125.1"
                     /translation="MNDELSSKKCTVCQAGAPVLKPKEIEEFQKKVDGNWRVIDNHHL
                     EREFKLTDFKASLDLTNKIGRIAEEEQHHPDICLSYGKVVARLFTHKIDGLHENDFIL
                     AAKIDKIVKARI"
     gene            713..862
                     /locus_tag="VFF28_00430"
     CDS             713..862
                     /locus_tag="VFF28_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44126.1"
                     /translation="MSTPAAFKNGIRPCKKIKEGYDLSSAIKEAVSDSGPHQAVIMRV
                     IGPYL"
     gene            936..>2203
                     /locus_tag="VFF28_00435"
     CDS             936..>2203
                     /locus_tag="VFF28_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZX44127.1"
                     /translation="MKNIIIKGAREHNLKNIDLELPRDKFIVITGLSGSGKSTLAFDT
                     IYAEGQRRYVESLSAYARQFLGLMNKPDVDSIDGLSPAISIEQKTTSKNPRSTVGTVT
                     EVYDYLRLLFARVGIPHCPEHGIKIESQSAQKIAGRIKSEIKGQVTVLAPIVRQKKGT
                     YEKLVADLGKEGFTRVRVNQKIQRTDEKVNLDRYKKHDIEIVVDRLDSKDSQRLNEAC
                     ENALKKSKDILLVMDDKGKDHLFSASMSCPRCGLSFEELQPRMFSFNSPFGACEECHG
                     LGIKMDFDPHLIIPDETKCIADGAVMLYRNAIDGWRGQYLGAVAKHLGFDVFTPMNKL
                     NEKQYKALMYGSDERIKFEMKMKGGEANWSHMGYWEGLVPQSERLYQQTESDYRKREL
                     EKFMRISDCPRCKGKRLKEKILAITINKRS"
BASE COUNT          750 a          419 c          478 g          556 t
ORIGIN      
        1 attatgttgt agaaggcaat atagataaga ggggcaaggt aaaacaaaag gtattgatgt
       61 atcttggcaa tgttgaccat gtaatcgaag tattcaagtt ctataactca cgcccaaagt
      121 aatttgtgcg caaagccagt cgttgattgg cctttttaat ccctaagatg atttaggcct
      181 aaaaatcctt gcaaagttat ttaagagttg aatcctactt aatcctatga acgatgaatt
      241 atcttctaaa aaatgcactg tatgccaggc aggggctccg gttttaaaac ctaaggaaat
      301 agaagagttc caaaaaaagg tagatggaaa ttggagagta atcgataacc atcatctgga
      361 aagagagttt aagcttactg atttcaaagc atcgcttgat cttaccaaca agataggaag
      421 gatagcagaa gaagagcagc atcatccgga catctgctta tcctatggaa aggtggttgc
      481 tcgcctattt acccacaaga ttgatgggct tcatgagaat gacttcatcc ttgcggcaaa
      541 gattgataag attgtaaagg ctcggatata atgctacgtt ggttattatt ctcatagttg
      601 ggtaaataag gattttgatc ccaagtcaat ctttcccacg gctctccagg gactagatga
      661 cactcgcgat tttttaaaag gtttggatgc cttagcaaca gtccccttaa tgttgtcaac
      721 accagcagca tttaaaaatg gaattagacc atgtaaaaaa ataaaagaag gatatgatct
      781 ttcatccgct ataaaagaag cagtttctga cagcggtcca catcaagcag taattatgag
      841 agtaattgga ccctatttat aattctcaat ttttttcaac aataccttta taaatcatct
      901 atctctactt caaaacttta cctagaggta tttgcatgaa aaatatcatc atcaaaggtg
      961 caagagagca caatctgaaa aacatagact tagagctgcc aagagacaag ttcatagtga
     1021 taacaggcct ttctggctct ggcaaatcca ctttggcatt tgacactatc tatgcagaag
     1081 gccaaagaag atatgttgaa agcctttcag cgtacgcaag acagtttctt ggcctcatga
     1141 acaagcctga cgttgatagt attgatggcc tctcgccagc aatctcgatt gagcagaaga
     1201 ctacatcaaa aaaccccaga agcacagttg gaactgtgac agaagtctat gactatctaa
     1261 ggctcttgtt tgcaagggta gggatccctc attgccctga acacggtata aagatagaat
     1321 cacaatctgc acaaaagata gcaggaagga taaaaagcga gataaaaggc caggtgactg
     1381 ttcttgcccc tatcgtaagg caaaagaaag ggacttatga aaaacttgtt gcagacttag
     1441 gtaaagaagg tttcacaaga gtaagagtta accaaaagat tcaaaggacc gatgaaaaag
     1501 tcaatcttga ccgttacaaa aagcatgaca ttgagatagt tgtagacagg cttgactcaa
     1561 aagacagcca gaggctgaat gaagcctgcg aaaatgcact gaagaaatca aaagatatcc
     1621 ttctggttat ggacgataaa ggaaaagacc atctattttc agcttccatg tcctgcccaa
     1681 gatgcggact ctcatttgaa gagcttcagc ccaggatgtt ctcattcaac agcccatttg
     1741 gcgcctgcga agaatgccat ggcctgggga taaagatgga ttttgaccct cacttgataa
     1801 tccctgacga gacaaaatgc atagcagacg gcgcagtcat gctttataga aatgctatcg
     1861 atggctggag aggccaatac cttggtgcag ttgcaaagca tctaggcttt gatgtattta
     1921 cacctatgaa caagctcaat gagaagcagt acaaggcact gatgtatggc tctgatgaac
     1981 gcataaagtt tgagatgaag atgaagggtg gagaggccaa ctggtcccat atgggatatt
     2041 gggaaggcct tgttccccaa tcagaaaggc tataccagca gacagaatct gattatagga
     2101 aaagagagct tgaaaaattc atgcgcatct ccgactgccc aagatgcaaa ggaaaaaggc
     2161 tgaaagaaaa gatccttgca ataaccataa acaaaagatc gat
//
LOCUS       DASRUK010000011        11091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2352544, whole genome shotgun sequence.
ACCESSION   DASRUK010000011 DASRUK010000000
VERSION     DASRUK010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 11091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11091
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2352544"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..794
                     /locus_tag="VFF28_00440"
     CDS             252..794
                     /locus_tag="VFF28_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44128.1"
                     /translation="MLLTEKKRMLYSEAFKDINSDLHEAFSQVRDEFEDHLIAVNENT
                     NEIQANYELLCNIDQKMNKMAERLDRLEMFLQQHGLKVEEKQEFPQIRLSKREQEIFL
                     VLYTLEEVKGSVTYLDIARKVCLPEDIVASYVANLVQKGVPITKKYVVNEAHLNLNPN
                     FKALQAKENVLQIEQRRLSL"
     gene            complement(918..1610)
                     /locus_tag="VFF28_00445"
     CDS             complement(918..1610)
                     /locus_tag="VFF28_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44129.1"
                     /translation="MAVNKKIFIIAFIITVLILVSILLSNSILNDKREQVLMERMDLM
                     VEEYEDMQALLFMSDFFGEEATCLALENVLEKMNKEVWTLGGKLDSYRQATEGFMRDP
                     FYQKQKETFNRKEVLYFSVLQKMQSMCPINQTIISFFYRKKDFCPDCDAQSFVISDIK
                     HDLEKVNLDHELPVFSFDADTNIASISLLTKYYGVDSFPCIVVGDRSYCGLHDKEELL
                     GILCANNSLSIC"
     gene            complement(1601..2839)
                     /locus_tag="VFF28_00450"
     CDS             complement(1601..2839)
                     /locus_tag="VFF28_00450"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZX44130.1"
                     /translation="MNPIYSIFVVLIWFLSTYFAVFLLLVLLGARKNLFLSPALTEDD
                     ELPKVSLILPAFNEEKTIAKSLDSLMVLDYPSDKMEILVVNDGSSDRTAEIVKDYADR
                     VILIDNKKNKGKAACLNQGIQACHGQLIVCMDADTMANPDVLKKVVPYFKDPSVASVT
                     VAINVKKPSNFLEKMVEIEYIVGLSLALKALSFFNAIHVTPGPFSIYRRGIFDKVGVF
                     DEQSITEDLEIAYRIQKSGYRIENCLATRVHTVVPSTFKGLYKQRKRWYSGAISTLMQ
                     HKDVVFNRKLGVFAYTVPFTFLLVILGLVLFVYSLFLVVSNLWKTLSFFSLTDFNFIS
                     YLTVFDFDIFAINALTAFGVISIVTTIIIAFVCLSLVRIRVYSKIPGLVGFIFLFFLY
                     QLFWATSIYSVIFGRRVKWR"
     gene            3204..3929
                     /gene="radB"
                     /locus_tag="VFF28_00455"
     CDS             3204..3929
                     /gene="radB"
                     /locus_tag="VFF28_00455"
                     /inference="COORDINATES: protein motif:HMM:NF020014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadB"
                     /protein_id="HZX44131.1"
                     /translation="MTKIPSGSKILDRMMEGGYEKDIITTIFGPAGSGKTVLCMLCAI
                     NAVRNGKKVIYIDTENNFSVERLKQICKFDYNKVLSNMVFLKPTSFGEQKNAFEKLKE
                     IVNNSIGLIAVDTISMLYRIEMGKNEDVYSVNKELGIQISYLSEITRKKNIPILLTNQ
                     VYADFDNKDKVKMVGGDLLKYSSKCLIELQLTTGGNRRCILRKHRSIAQEREITFKIV
                     EGGIIGTKEGLMKKILPAQYFKV"
     gene            3999..5540
                     /locus_tag="VFF28_00460"
     CDS             3999..5540
                     /locus_tag="VFF28_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HZX44132.1"
                     /translation="MATSDKTKEFTKKQDDIIINKEGAKRQHESGKLTAEERLALLLD
                     DNSFTEIDEFVELRSHDYGLQEKKKPRDGVITGYGTVNKKPVCIYAQDFTFMGGSMGE
                     MHNKKIANIMELALKIGCPIIGLLDSGGARIQEGIDGLDGGGKIFQLNTLCSGVIPQI
                     SAIMGPCAGIAVYSPALTDFTFMVDKTSYMFITGPDVVKSVTGENIDFMGLGGAETHS
                     KKSGVSHFIAADEKECILKIKKLLTYLPQNNLEDAPLLDMGDSAERQTASLKNIIPED
                     QKKSYDMKEVIKEVVDKNSFYEIQAEYAQNCIIGLARLNSVAVGIVANQPCVIAGCLD
                     INSSDKISRFVRFCDAFNIPIINFVDVTGYLPGTEQEHNGVIRHGAKILYAYSEATVP
                     KISLVLRKAYGGAYIALVSKDMGYDYVISWPSAEIAVMGSDQAVNIINKKEKASKDWE
                     KIKSEKIKEYSDKFLNPYVAASEGKVDKIILPKDTRGALIRAIEMLASKRDKRPSKKH
                     GNIPL"
     gene            5697..6083
                     /locus_tag="VFF28_00465"
     CDS             5697..6083
                     /locus_tag="VFF28_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HZX44133.1"
                     /translation="MQELKINIDGKEYIVKIEEIETGKLKVHFGGEAFEVETKSDVNK
                     ELFDKIEERVNKKEKNAITAPLPGVVFSVDVKVGDEVKQGDKLMSLMAMKMENNITAP
                     FDGKVKDIKAKKDDTVNKGDLLLVIE"
     gene            complement(6305..6448)
                     /locus_tag="VFF28_00470"
     CDS             complement(6305..6448)
                     /locus_tag="VFF28_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44134.1"
                     /translation="MNIDEMEEMAYLLESDNLLELNRKISSHLRKRGIKIREYKWNEL
                     EAS"
     gene            complement(6445..6681)
                     /locus_tag="VFF28_00475"
     CDS             complement(6445..6681)
                     /locus_tag="VFF28_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44135.1"
                     /translation="MGFLDNTPLLKRIFELGYTMFLIILLSTLVGLILAFPVMWLWNF
                     IFKNFPQLRINVFQAWALNVLAGILFGKTGEKGK"
     gene            6781..7164
                     /locus_tag="VFF28_00480"
     CDS             6781..7164
                     /locus_tag="VFF28_00480"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZX44136.1"
                     /translation="MENITIHRVRYSDTDHFGVVYYARYLDWFEAGRTEILREKGISY
                     AGLEKKGFFAPVVEVKVNYKKPARYDDVINVQTSVEHVGNSSIKFAYKVLRDKEILAE
                     AYTVNVFIDKEMNPVRIPDEVRDIL"
     gene            complement(7148..7837)
                     /locus_tag="VFF28_00485"
     CDS             complement(7148..7837)
                     /locus_tag="VFF28_00485"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HZX44137.1"
                     /translation="MKGYKVRYFEKLDSTNKKAISYPVGSVIVAKEQSGGKGRFRRKW
                     CSSRGGIYMSIVTDAYSPNYLTLIAAIAVNRSIEDACGIKTMIKWPNDLLFKKKKISG
                     ILTVISQGKAVVGIGINTNNALPGSLYGKAGSLQQFTGSKIDNRLIINNIISCLDHHI
                     GWLKDKRYDKILSEWKNRSFIGHKIIVKTLKKTVKGTAYDIDEDCFLIVKDNKGKKER
                     IIEGDITIVYP"
     gene            complement(7824..9632)
                     /gene="accC"
                     /locus_tag="VFF28_00490"
     CDS             complement(7824..9632)
                     /gene="accC"
                     /locus_tag="VFF28_00490"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HZX44138.1"
                     /translation="MTKKILDKAHDSVPYVYQEIERVIRSQLVIPEKGEELLQSPNVG
                     SFSDVIAKNLAKKLPNMSGKQVDKMINEKSSKSFLDMLARGLTNAEAEKEYTKVSKKK
                     MFKTVMIANRGEIALRVFRACMELGIETAIVYTKQDKDTLAVRFANKKYYIGSKTSDY
                     LNYKKIVKKAKKAGADAIHPGYGFLSENVDFADLCEKKKIKFIGPTSNAIRLMGNKDM
                     AKQTIIKLDIPTIEGTKALKDEKEAIESAGRIGYPVILKAVSGGGGKGMRIVKEAKDI
                     PAGFKSAQSEAEASFGSGAMYMEKYLEEPRHVEFQILIDDYGNAVHLGERECSIQRRH
                     QKLIEESPSPYLDERLRAKIGEAAIKIVKTVKYHGAGTVEFLIDKNKNFYFMEMNTRI
                     QVEHGITEMVTGVDLIKEQIKIAAGAKLAFKQDDIKIDGCAIECRINAESPSEDFCPQ
                     AGTIVNYLPPGGPGIRVCSSCYSGHVVSPHFDSLIAKLMCKGTNRQEAIAKMNRALEE
                     FIIEGISTTIPFHKKVLSSKGFMEGDTTTNFIDKYHVVEQLKKEKPKKTGLSKKEKML
                     IISTAVSKYLEKGPLHTKQSAWKMAAKQELMHNERL"
     gene            10038..11069
                     /locus_tag="VFF28_00495"
     CDS             10038..11069
                     /locus_tag="VFF28_00495"
                     /inference="COORDINATES: protein motif:HMM:NF024918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HZX44139.1"
                     /translation="MNILYGVCGEGLGHSSRAIAIADHLIKKGHKVKIITYGQAYTAL
                     KGKYDLFKAKGLHLIFKGNVLEKRRTLTYNLRHFTRNLKRLKEFDRLVKGFRPDVCIT
                     DMEAIVPALSRWYSLPLISFDNQHRICNCHLKVPKRYYPDYLLAKEVIKTITRGVKYF
                     IITDFVHLPVKKRYESRTVIVPPVIRKEIIDLKTRNSGKILVYLTKKNSPILKVLSGI
                     KQQFVIYGYNQEKRKKNLTFRKRDSFLSDLASCRAIIASAGYTLMSEALYLKKPYLAL
                     PLEGHFEQAINAFFLKEAGFGDYSENLTEKDVIYFMYKLEDYRRSILLHNFDSNLVFN
                     AIDMALKSV"
BASE COUNT         3408 a         2349 c         2252 g         3082 t
ORIGIN      
        1 agagatggtg gtagaagagc aaggcctttg ctctaaatca tctttttacc gtatgcttga
       61 agagatagaa gaagaccctg agattggagt catcaagtcg ggaaaagaga agcattacct
      121 ctccaagatg agtcagagaa atgtttaatt gcactattga aaaatatttt acataatata
      181 ttttgcaaaa caggaaacta tttaaacccc tttttggaac tagttgttaa gtagttcggg
      241 ggtgtatgta ggtgttactg acagaaaaaa agaggatgct atactcagag gcgtttaagg
      301 atatcaactc agatctccat gaggcttttt cacaggtaag agatgaattt gaagatcatt
      361 tgattgccgt taacgagaat accaatgaga tacaggcaaa ttacgagctt ctatgcaaca
      421 tagaccaaaa gatgaacaag atggcagaaa ggcttgaccg tcttgagatg ttccttcagc
      481 agcatggcct aaaagtcgaa gaaaagcagg aattccccca gataaggctg agcaagagag
      541 agcaggagat attcctcgtc ttatacacac tggaagaagt caaaggttct gtaacctatt
      601 tagacatcgc aagaaaagtc tgccttccag aggatatcgt cgcttcctac gtcgcaaacc
      661 ttgtccagaa aggcgtgcct ataacgaaaa aatatgttgt gaacgaggcc catttgaacc
      721 ttaatccaaa ttttaaggca ttgcaggcaa aagaaaacgt cctgcagata gagcagagaa
      781 ggctatcact ttaaatcatt tatttgttgt aaataccctc ccgcttgcgg tgactgggtt
      841 attgttatgg ctcgccacgt tgagggtatt tacaacacta aaaatacccc gccccttggg
      901 gcggttggga tttttattta gcatattgac aaagaattat tggcgcataa gatacccagc
      961 agctcttcct tgtcatgaag cccacaatat gatctatcgc ccacaacaat gcaagggaaa
     1021 gagtcaacgc cgtaatattt tgtcagcaat gatatggatg caatattagt atcggcatcg
     1081 aacgaaaaga caggcaattc atggtctagg ttcactttct caaggtcatg ctttatgtcc
     1141 gatataacaa aagattgagc atcacagtca gggcagaaat ctttttttct gtagaaaaac
     1201 gaaatgatcg tctgatttat cgggcacatg ctctgcatct tttgaagcac agagaaatat
     1261 aacacttctt tgcggttgaa tgtctctttc tgcttttggt agaaaggatc ccgcatgaac
     1321 ccttcagtcg cctgcctgta ggaatcaagc ttgccaccaa gcgtccatac ttccttgttc
     1381 atcttttcaa gcacgttttc aagcgcaaga caggtggctt cttcgccaaa aaaatcagac
     1441 atgaacagga gggcctgcat atcttcatat tcctcgacca tgaggtccat cctttccatg
     1501 agcacctgct cccttttgtc attaagtatc gagtttgaca acaggatgga gaccaaaatc
     1561 aacactgtaa ttataaacgc tataatgaaa attttcttgt ttaccgccat ttaactctcc
     1621 tcccgaatat gactgaatat atcgacgttg cccagaacag ctggtagaga aaaaacaaaa
     1681 atatgaaacc cactaatcca ggaatcttgc tatagacccg tatccttaca agcgacaggc
     1741 agacgaatgc aataattata gtagtgacta tcgatatcac cccaaacgct gtaagggcgt
     1801 ttattgcgaa tatgtcaaaa tcaaagacag taaggtacga aataaagtta aaatcagtca
     1861 aagaaaaaaa ggagagggtc ttccagaggt tggatacaac aaggaaaaga ctgtaaacaa
     1921 agaggacaag gccaaggata accaacaaaa aggtgaaagg cactgtataa gcaaacaccc
     1981 ccagcttcct gttgaatacc acgtctttgt gctgcatgag agtcgatatg gcaccagaat
     2041 accatctttt cctttgctta tagagccctt taaacgtact tgggaccacc gtatgcaccc
     2101 ttgtcgccag gcagttctct atcctatagc cacttttttg tatcctgtaa gcgatctcaa
     2161 gatcttcagt tatactttgc tcatcaaaga cacccacctt gtcaaatatc cctcttctat
     2221 atatcgagaa aggcccaggg gtgacatgga tagcattaaa aaacgacaat gcctttagtg
     2281 caagggaaag accaactata tactcaatct caaccatctt ctccaagaag ttggagggct
     2341 tttttacatt aatcgcaaca gtcaccgatg ccaccgaggg atccttaaaa taaggtacga
     2401 ccttttttag aacatccgga tttgccatgg tatcggcatc catacagact ataagctgcc
     2461 catggcaagc ctgaatgcct tggtttaagc aggcggcctt tcccttattt tttttattgt
     2521 ctatcagtat aaccctgtca gcataatcct tcactatctc tgcagtccta tcgctactgc
     2581 catcattaac cacaagaatc tccatcttgt cgcttggata gtcaagcacc ataaggctgt
     2641 ccagcgattt tgcgatggtc ttttcttcat tgaaagcagg cagtatcaaa cttacctttg
     2701 gcagctcgtc atcttcagta agagcaggac ttaagaagag gttttttctt gcacctaaaa
     2761 ggaccaataa aagaaaaact gcaaaatagg tggaaaggaa ccagattaac accacaaata
     2821 tgctatagat tggattcatc ttttgatcct gctgaatgat tttagcttta aatagtttac
     2881 gcaaacttcc ccttgaccaa taaaaaatcc caaaccgcta gccgagctgc ggggtatttg
     2941 tcaaaaactc cagtattaat gcaaagcttc agtgaggagt ttttgagcat gtcagaaatt
     3001 ttcttcctgc tgggcacttt tagcaaggaa aatttctgac attttgagcc tcaaactggg
     3061 ttattaagcc gcagcaacag aaacttcgtt tcttagttgc gctcacttcg ttcggcaaca
     3121 agctacgggg tatgaaccca agtcttgagc aatcaagcgc acactgcaaa aatttatatc
     3181 ctttgccaca ctttctcttt catatgacaa aaataccttc cggaagcaag atacttgaca
     3241 ggatgatgga aggaggctat gagaaagata taatcaccac aatctttggg ccggccggat
     3301 caggaaagac cgtcctatgc atgctctgtg ccatcaatgc agtaaggaac ggaaaaaaag
     3361 tcatctatat agatacagag aacaactttt ctgtggaaag gctcaagcag atatgcaagt
     3421 ttgactataa caaagtgctt agcaatatgg tcttccttaa gccaacttcg tttggtgagc
     3481 aaaagaacgc ttttgaaaag cttaaggaga tagtaaacaa ctctataggc ctaatcgcag
     3541 tcgacactat ctctatgctg tataggatag agatgggaaa aaatgaggat gtctattcag
     3601 tgaacaaaga gctgggtatc cagatctcat acctatccga gataacccgc aagaaaaaca
     3661 tccctatcct cctaaccaac caggtctatg ctgattttga caataaagac aaggtcaaga
     3721 tggtaggggg ggacctgctc aaatacagct caaaatgcct tatcgagctc cagcttacta
     3781 ctggcggcaa taggaggtgc atacttagga aacaccggtc tattgcccaa gagagggaaa
     3841 taaccttcaa gatagtagaa ggaggcataa tcgggacaaa ggaaggcttg atgaagaaaa
     3901 tcttgcctgc ccaatacttc aaggtttgaa tttacgacta taaactcctt ttagcaatct
     3961 ttttaaacaa acgtttattt tcaacacctg gcaacaaaat ggcaaccagc gataagacaa
     4021 aggaatttac caagaaacag gacgacatca ttataaacaa agaaggagca aaaaggcagc
     4081 atgaaagcgg aaaacttact gctgaagaaa ggcttgccct tcttcttgat gataattctt
     4141 ttacagagat agatgaattt gtcgagctca gaagccacga ttatgggctg caggaaaaga
     4201 aaaaaccccg agacggagtt atcacaggct atggtacagt caacaaaaaa cctgtatgta
     4261 tatatgcaca ggattttacc ttcatgggcg gctctatggg agagatgcat aacaaaaaga
     4321 tagccaacat aatggagctt gctttgaaga taggctgccc tatcatcgga ctacttgatt
     4381 caggaggggc taggatccag gaagggatag atggacttga cggcggagga aaaatcttcc
     4441 agctgaacac cctatgctca ggtgtcatcc cgcagatttc tgccatcatg gggccttgcg
     4501 caggcattgc agtatacagt cctgcactca ctgattttac atttatggtc gacaagacga
     4561 gttacatgtt catcacaggg cctgatgttg taaaaagcgt cacaggtgaa aatatagact
     4621 ttatgggcct tggcggagct gaaacccatt ctaaaaagag cggagtgtca cattttatcg
     4681 cagcagacga gaaggaatgc atactaaaga taaaaaaatt gcttacctat ctgccacaaa
     4741 acaatctaga ggacgcacct ttgcttgaca tgggagacag cgcggagcgg cagactgcca
     4801 gccttaagaa tatcatccct gaagaccaga agaaaagcta tgacatgaag gaagttatca
     4861 aagaggttgt cgataagaat tcattctatg aaatacaagc tgagtacgcg caaaactgca
     4921 taatcggcct tgcaagactt aacagcgtgg ctgtcggaat cgtcgccaac cagccttgtg
     4981 taattgcagg atgccttgat atcaacagtt ctgacaaaat atcaaggttt gtcagattct
     5041 gtgatgcctt caacataccc atcataaact ttgtggatgt cacaggatat cttccaggta
     5101 cagagcagga gcataatgga gtcataaggc atggtgcaaa gatactatat gcctattctg
     5161 aggcaactgt accaaagatc agccttgtgc tcagaaaagc atacggcgga gcttatatcg
     5221 cgcttgtcag caaggatatg ggttatgatt atgtgatttc atggccttct gcagagatag
     5281 cggttatggg ttcagaccaa gcagtcaata tcatcaacaa gaaagaaaag gctagcaagg
     5341 attgggaaaa aataaaaagc gagaagatca aggagtattc cgacaaattc ctaaatccat
     5401 atgttgcagc aagtgaaggt aaggtcgata agatcatcct gccaaaagat accagggggg
     5461 ctttaataag ggctatcgag atgcttgcat ccaagcgaga caaacggcct tcaaaaaagc
     5521 acggaaatat cccactgtaa caacagaatt ccaccaatct aggattggtg gtcgagtgga
     5581 attctgtgtg ctcaaacacc gcctcactcg cggaattctg agcatcaaaa accacttggc
     5641 agttcacaat gaaagtaaaa aataagtgtt atcaattgca aagcggtttt tgacaaatgc
     5701 aggagctaaa aataaatatc gacggcaagg aatacatagt aaagatcgaa gagatagaga
     5761 caggcaaact taaggtccat ttcggcgggg aagcgtttga ggtcgaaact aaatctgatg
     5821 tcaataaaga gctgtttgac aagatagagg aaagggtaaa caagaaagag aagaatgcga
     5881 taaccgcacc acttccaggt gttgtttttt ctgtcgacgt aaaggtggga gatgaggtaa
     5941 aacaaggcga taaattgatg tctcttatgg caatgaagat ggaaaacaac atcacagcgc
     6001 catttgacgg caaggtcaag gatatcaagg ccaaaaaaga cgacacagtc aataaaggag
     6061 atctgctttt agttattgaa taaaccttga aacaatgaaa ttccaccagt ctatttgact
     6121 ggtggagtga attgtgctca aaaaccacta tctggtagaa cactaaaaaa tttaccattg
     6181 caaatttttg gtgcaccaaa aatcctatgg attttttagt gtttcaagcg tcaaaaacca
     6241 cttaaccgtc atgccaccag tctatagcga ctgaaaggag cggtgactgg tggtttttga
     6301 caaattaaga tgcttctaac tcattccact tatattctct tatctttatc cctcttttcc
     6361 tcagatggct agaaatcttc ctattaagct caagcaggtt gtcagactca agcagatagg
     6421 ccatctcttc catctcatcg atattcattt tcctttttca cctgtcttgc caaacaatat
     6481 ccctgctagg acgttgagcg cccatgcctg aaaaacattt atcctcaatt gcgggaaatt
     6541 tttgaaaata aaattccaaa gccacatgac ggggaacgct agtatcaggc caaccaatgt
     6601 tgacaaaaga atgatcaaaa acatagtgta gccaagctcg aatatccttt ttaacaatgg
     6661 cgtgttgtct aaaaacccca tcctcaaatc tgttaatgtc cgtcaattta taaagattat
     6721 tgaaaataaa ttggtaattt tggcaaaaaa tatatacttc agcggctttc ttctgggtta
     6781 atggaaaaca taaccatcca tagggtgagg tattcagaca ccgaccattt tggcgtagta
     6841 tactatgcca ggtacctcga ttggtttgag gctggcagga ccgagatact cagggaaaaa
     6901 gggattagtt atgcaggcct ggaaaagaaa ggtttctttg cacctgtcgt ggaagtcaag
     6961 gtcaactata aaaagcctgc aaggtatgat gatgttatca atgtacagac aagtgtcgaa
     7021 catgtaggga actcaagcat aaagtttgcc tacaaggtat tgcgggacaa ggagatcctt
     7081 gccgaagcat acactgtcaa tgtttttatc gataaagaaa tgaatcctgt acgaatacct
     7141 gatgaagtca gggatatact atagtgatgt ccccttcaat gatcctttct ttttttcctt
     7201 tgttatcttt aacaatcagg aagcaatcct catcgatgtc ataagcagtg cctttgacag
     7261 tcttttttag cgtctttact attatcttat gcccaataaa cgagcgattt ttccattcgc
     7321 taagtatctt atcatatctc ttatctttta accatccgat atgatggtca aggcagctta
     7381 tgatattgtt tataatcagc ctattatcta ttttactgcc tgtaaattgc tgcagcgaac
     7441 cagctttgcc atacaaggag ccaggcagag cgttgttggt atttatccct atacctacca
     7501 cagctttacc ttgcgagata accgtcagga tccctgagat cttcttcttt ttgaacaaca
     7561 ggtcatttgg ccatttaatc attgtcttta tcccacaggc atcctcaata gaccggttta
     7621 ctgcaatggc tgcaatcaat gtaaggtagt ttggcgaata agcatcagta actattgaca
     7681 tatagatgcc tcctcttgac gagcaccact tacgcctgaa gcggcctttg ccaccacttt
     7741 gctcctttgc aactatcact gagcctactg gataagaaat cgctttttta ttggtagagt
     7801 ccaacttttc gaaatacctt accttatagc ctttcattat gcattaattc ctgctttgca
     7861 gccatcttcc atgcagactg cttggtatgc agaggcccct tttcaagata tttcgagaca
     7921 gcggtagaga tgataagcat cttctctttt tttgaaagtc cagtcttctt tggtttttcc
     7981 ttctttaact gttcgactac atggtacttg tcaatgaaat tggtagtggt atcaccttcc
     8041 ataaatccct ttgaactcaa caccttttta tgaaaaggga ttgttgtaga tataccttca
     8101 atgataaatt cctccagagc cctattcatc tttgctatag cttcctggcg gtttgttccc
     8161 ttgcacataa gctttgcaat gagcgaatca aagtgaggac ttacaacatg gcctgaataa
     8221 cacgaagaac agactcttat acctggcccg cctggcggaa gataattaac gatagtgcct
     8281 gcctgagggc agaaatcctc agatggagat tctgcgttta tcctgcattc aatggcacag
     8341 ccgtctatct taatgtcatc ctgcttgaat gcaagctttg ctcctgctgc tatctttatc
     8401 tgctccttga tgagatctac accggtgacc atctcagtaa taccatgctc gacctgaatc
     8461 cttgtgttca tctccatgaa atagaagttc ttgtttttat caatcaaaaa ttcaacagtg
     8521 ccggcaccat gatacttgac agtttttact atcttgattg ctgcttcgcc aatctttgcc
     8581 cttaatcttt catcaaggta aggagagggc gactcttcga taagcttttg gtgcctcctt
     8641 tggatgctgc attctctctc gccaaggtgg actgcattac cataatcatc tatcaatatc
     8701 tggaactcga cgtggcgcgg ctcttcaaga tatttttcca tatacatggc gccactacca
     8761 aacgaagcct ctgcctcaga ctgagcgctc ttaaaacctg cgggaatatc ctttgcctcc
     8821 tttactatcc tcataccttt gccgcctccg ccagagacag ccttcaatat caccggatat
     8881 cctatccttc cagcagactc aatcgcctct ttttcatcct tcaacgcctt ggtcccttcg
     8941 atggtaggta tatcaagctt gattatggtc tgttttgcca tatctttatt gcccataagg
     9001 cgtatggcgt tggaggtggg gccgataaac ttaatcttct ttttttcaca aagatctgca
     9061 aagtcaacat tttcagaaag aaagccataa cctggatgga tagcatcagc acctgctttt
     9121 tttgccttct ttactatctt cttataattg aggtagtcag aagtcttgct gccgatatag
     9181 tacttcttat ttgcaaacct tacggcaaga gtgtccttgt cctgctttgt gtagacaatc
     9241 gctgtttcta tgcccagctc catacatgct ctaaacaccc ttaaggctat ctctccccta
     9301 tttgctatca tcactgtctt gaacatcttt ttcttgctca ccttagtata ttctttttct
     9361 gcttcagcat tggtcaggcc tcttgcaagc atgtccagga atgattttga agatttttcg
     9421 ttgatcatct tatctacctg ttttcctgac atgttaggaa gtttttttgc aagatttttt
     9481 gcgatgacat cggaaaatga gcctacattt gggctttgca aaagctcttc acctttctca
     9541 ggaataacca actgagacct tatcaccctc tcgatctcct ggtagacata agggacagag
     9601 tcgtgggctt tatctagaat cttctttgtc atcaaataag ccctctgcga agtcgaaagg
     9661 aaacagttga gagaccttgt taaaaattta tccggcatgg gatactagta gttatttttc
     9721 cgtttaaaaa tgttatcttt ggggcaggtt aacatgtcag aaatcgctat tggcgcacac
     9781 gctggcgttt taacccagag taagccagcg atttctgagt gtcggaaagc tttgctttct
     9841 gacaaaccag aaatttgctt ctcaaatttc tcagttcatg ctcaagaaac acgcccccgg
     9901 gctttcaccc ggcggttctt gacatgtcag aaatcgccta tggcacatac tttgctcatg
     9961 ctttgtctat gacacatcaa gcaatttctg agcatttcag cgattgtttt atatacagat
    10021 tataccaaaa tagtattatg aatatccttt atggagtctg tggagaaggt ttgggccatt
    10081 cttcaagagc tatagcaatc gcagatcatc tgataaagaa gggtcataag gtaaagataa
    10141 tcacatacgg acaagcttat acagcactga aaggcaaata tgacctgttc aaagctaaag
    10201 gcctacatct aatcttcaag ggcaatgttc ttgaaaagag aagaaccctt acctataact
    10261 taaggcattt tacccgcaac ctaaagagac taaaagaatt tgacagattg gtgaagggat
    10321 ttaggccaga cgtctgtatc actgatatgg aagcaatagt tccagcactc tcaagatggt
    10381 acagcctacc tttaatctca tttgacaacc aacatagaat ctgcaattgc catctcaagg
    10441 tacctaagag atattatcct gactatctcc tagctaagga agtaatcaaa acaatcacaa
    10501 ggggggtaaa gtactttata ataacagatt ttgtacattt gcctgtcaag aaaagatatg
    10561 aatcaaggac tgtaatagtc ccgccggtca tccggaaaga gataatagac ttaaagacaa
    10621 ggaatagcgg caagatccta gtctatctca ccaaaaagaa cagccctatc ctcaaagtat
    10681 tgtcgggcat aaaacaacaa tttgtcatat atgggtataa tcaggaaaaa aggaagaaga
    10741 accttacctt cagaaagaga gatagttttc tatctgacct tgccagctgt agagctatca
    10801 tcgcttctgc aggctatacc ttaatgtctg aagcgttgta cttaaagaaa ccttatcttg
    10861 ccctgcctct tgaaggacat tttgagcagg caataaacgc tttttttctt aaggaagcag
    10921 gatttggaga ttatagtgaa aatcttactg aaaaggatgt tatctacttt atgtataagt
    10981 tagaggacta cagaagaagc atcctgttac ataactttga tagcaatcta gtctttaatg
    11041 ctattgacat ggcattgaag tctgtctaaa agtttgtggg ctaggtctcc c
//
LOCUS       DASRUK010000012        13634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1350794, whole genome shotgun sequence.
ACCESSION   DASRUK010000012 DASRUK010000000
VERSION     DASRUK010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 13634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13634
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350794"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            420..911
                     /locus_tag="VFF28_00500"
     CDS             420..911
                     /locus_tag="VFF28_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44140.1"
                     /translation="MANYYIKANRQEWKYNNQHTDWDNFPLNHKQDFATFTKNQLYWK
                     GLKKGDIIIGHSSYLSSNIKGKIKFTPLPRISAIAIVTNEEHYSKKLDSDSVTLKKVI
                     EFSPIKISIDIINKNKLYNAEPFRLGTNRCTITKLSDEEFEKVIKLIEQYHPNIKREL
                     KSI"
     gene            complement(1191..1409)
                     /locus_tag="VFF28_00505"
     CDS             complement(1191..1409)
                     /locus_tag="VFF28_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HZX44141.1"
                     /translation="MLVNLKNSKDEVYASSLAKQIDCTYSHVVKILQEMEKSGLINFE
                     RQGRLKLLTLTKKGQEVADHIDNIRLSL"
     gene            1843..3687
                     /locus_tag="VFF28_00510"
     CDS             1843..3687
                     /locus_tag="VFF28_00510"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HZX44142.1"
                     /translation="MIDKEEILFPYPEVREIQSDMIKEVMECIENGENALIHAPTGLG
                     KTVASLGPALSVAMKKGLTIFFLTSRHTQHVLAINTLRDIKKKHGVSVVCADIIGKQH
                     MCALGEVENLYSSEFRDYCKKMRDDKMCEFYINTKNTSGRPTLKALSILEELKHLSPM
                     HVEEVVSIASKEKLCPYEMSLLLAKEAKVIVADYYCIFNPEIRRSFMNKTEKELETSI
                     VIVDEAHNLPRRCRELMSQNLSTVMLKRAIKEAGKFGHFEAMDKLQGMNNFLEKLTLT
                     IEEFKDEKLITKKEFMALPNYDDLAGEFALIGDEVREKQKKSYIASIAVFLDKWSGED
                     RGFARILQRKGDVANLIYKCLDPSFISKEVVDNARSVICMSGTLTPTYMYKDILGFEN
                     AREMVFKSPFPSNNRLSLIIPETTTRYSKRGAGEFEKIAAVCSNIVNLVPGNSMVFFP
                     SYDLRDKVYAFLKCNKEIMVEKPRLTKEEKNEMLNEFKKRKDKGACLLAVTSGSFGEG
                     VDLPGDFLKCVVVVGLPLEKPSLEVRELIDYYDAKYGKGWEYAYLIPAMIMIMQNAGR
                     CIRSETDRGVIAFLDERYSWEQYYKCFPPDHKVKISKLYLEKIKGFFD"
     gene            3756..4166
                     /locus_tag="VFF28_00515"
     CDS             3756..4166
                     /locus_tag="VFF28_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44143.1"
                     /translation="MVVLFDRFNLPEDVYEVVFATKQQVIVARLLVDMIKEKNSEITK
                     TEMSFFATQLHEGKLVTDKIQEEPYRGKRVKISYNKRQFYDRILTPMKSMGIIDYDLY
                     KKTYRLSDKFNKEMIRIGLMWLKELRKDPYMIEK"
     gene            4326..4856
                     /locus_tag="VFF28_00520"
     CDS             4326..4856
                     /locus_tag="VFF28_00520"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZX44144.1"
                     /translation="MKIVSKRKAIELLKKYANSEESFKAILAHTRAVAKLAVEIAKDV
                     PGVDVEFIEGAALLHDIGRFKAKETVKHGIAGRDILLSEGLPEYASVAERHLGAGISK
                     EDIIQQKIDLPLKDYVPVTKEEKIITHADNLIEINRVISVEKTVERFRKEVGEEAARR
                     VKRLALEVENMRKIDE"
     gene            4890..5117
                     /locus_tag="VFF28_00525"
     CDS             4890..5117
                     /locus_tag="VFF28_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44145.1"
                     /translation="MKKKTKAKPASKKKVEKLKPAKASVDVGFEEVTVKCPNCGKVFR
                     VIKSSGFSMEGMLCQRCAAGGGIGLDQDADF"
     gene            complement(5114..5242)
                     /locus_tag="VFF28_00530"
     CDS             complement(5114..5242)
                     /locus_tag="VFF28_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44146.1"
                     /translation="MDEFNNLKKKRKELDIDDSDIPIHEKYVKEEDIKRMKKKRKD"
     gene            5501..5767
                     /locus_tag="VFF28_00535"
     CDS             5501..5767
                     /locus_tag="VFF28_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44147.1"
                     /translation="MDDNTSEGDKDEDIYNAQERDELMEDDHISPVEQGFMEGATEDG
                     QGAKCRYCGSVLMDNFVEQRIDSEIYRFCSIEHAERFKEREKKE"
     gene            complement(5958..6155)
                     /locus_tag="VFF28_00540"
     CDS             complement(5958..6155)
                     /locus_tag="VFF28_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44148.1"
                     /translation="MYLRKRVIGDLYLASSLLFITLAFLYFGFTNNGLPFLIIGLALA
                     GFGIKTKHTLIKQEKKARKKI"
     gene            complement(6181..6627)
                     /locus_tag="VFF28_00545"
     CDS             complement(6181..6627)
                     /locus_tag="VFF28_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44149.1"
                     /translation="MRKGQAAMEFLMTYGWAILVVLIAIGALAYFGVLNPEKFLPEKC
                     VISAGSGIWCDDFSIDSSSQVRLNLKNILTDDMSSVAVTLNNTAGGGTGICTVSGQTI
                     SADATTAVTLTCSPALSEKIKADLKLDYTVSSFAKTTTGTITGRVP"
     gene            complement(6760..7752)
                     /locus_tag="VFF28_00550"
     CDS             complement(6760..7752)
                     /locus_tag="VFF28_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44150.1"
                     /translation="MRKHSMMRSRKSMYFTVVTIAFLFIFTFVFLAQNYRQLGEKMGV
                     VEMRVLSMNDFVKDIERDSERGLYISSYRALLALEEYIITNGTFLSDVENSFREAIFN
                     GTVQNQSSYFMSESTLPLWIEKIEDEAANFNIGLEITPGNLTVYQKDPWHVRIGANFS
                     FALNDTSAIASWQRDTYIEASLSLIGFEDPLYIIYSYGRISNNLNATPYENNFTYDSG
                     GQIKVSNLLDHLNHSYYSANPAAPSFLMRLKNDLGPSPYGIESMVDLLKLSKQGLNID
                     EDSSIIDYYYWQGSSNGDYRVNQTPSWFKIDYAHLQKYNASSISCQIGSPGCNI"
     gene            complement(7749..12137)
                     /locus_tag="VFF28_00555"
     CDS             complement(7749..12137)
                     /locus_tag="VFF28_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44151.1"
                     /translation="MRKAVFFSLDALIAAILIITVVVLTPLFYLKEEENPQNIFYSSD
                     VIQLLSSIRIDELNNSGIALFRQRENLTDVNKTILEQILRFQVEGDEDKASELLNLIL
                     SGLVPDYYGMGVWIEGFGEPVLSSNLSYSGQLISSKQMVSGIERGRAIEGLAARALLT
                     DINERFANEYIFFGGYEGDGNLTKEFSLPSTLSSVDSAYMEIDAGSSFNLYFNGNYSG
                     AYVPPGSMAAGKWDINSSYLQYFQPGANSIVVGFNGTRKYIGGGYIRLGHTSAELSVD
                     NSQDRYTFPGVDGIINIYSSLFVPGALDSMGVYLHYDSNYEIFLALGGETVYSYNSNG
                     ENSVNITDSELRLMLNYTGIGKRTLPLRLGLRNVSLAFGGRSDSVLITDRTGSMEACD
                     VDTNCTFGICDTYAPCHDRRDRVAQNSDRTFLNEILSVEGNSVGMIGYGKELNPVCSF
                     TDIIADNATLQDSVDDYYNEWCGYTCISCGIAAATQILTDNKLLYGTTVKQAQDTSQV
                     HLGDTGSVSTTKSLEIEVNHSNFIKAALTIFGKGVDITFGYKDCVFFNDHYLGRMCIP
                     SDDGSSGWHTCSYPINQEWLPTSNASIGQWVTTSKEDFEACSLTRIDLDNAPGDAVMN
                     TSQLAGQQGLFSDNFDDGNSNGWSESGGNWAVRDQNGYTWQVGENGIYRQRQTAYNSR
                     SIAGNQSWQDYIVEAKVRINDADPMGIYFRYNSTHFYRFEFADGYPYYGFRLYRYTPS
                     GYTLLGYDYSFNPGRGVWYKMKAKVEGTSIKCYFDRGSGYELIFDTSDSSGITAGKIG
                     IYSSYSVNADFDNINVSSLSLSESYVLSQVLDAGRIAGWEGLSWTQALLNNTNLSIDI
                     RSGNTAVPDSSWDNFYFQEEPYSQETPHNTTVQVKSDISQSSSRYIQWRAKLTSSQQN
                     LTPALQDVSVNYSVDTKTNFVTITGGTSSGCFETSGDNDDWDLKDVTLKVWESPSNTS
                     KQSYASPSQVNLDDGAESATTVFDTGVAKEEIKSAYLAMEAYGVAPSYYDCVYVNGHY
                     IGRLDYPETSGSSVWENITLEIPTLAFDSAFSVRITSGNTYGCDQKSGDNDAWSYRNL
                     NLTLRWNNETSGYNRFMSMLVMSDGEANTKIGHTSAYNATGAGIEAVEKACEAFEDYG
                     IRIYSVTFGSGGDEELMEDIACCDDCSHHYSANNAEELLEIYKEIAQEIVKLQYEAQI
                     VNVTGNISTVLFPDSYIDYTYTKDSGPQVYNRIPITLETARMDNNITEGSFYIPQNTI
                     VSDAKITSYSSDRWTDKALINQAGSWTAFYNLSEFGYTYHPLGDPYIINIPVELISQG
                     TNQVRISTGINPYNSTGGSPDDRVIYTVEADLDVNYTGIFETAQGCIWTLAFEDDSEV
                     QMPIPDSYEGSNSCVFDNSTDCSEFSDDAINNALCYLFRQLDFDDNGKLFVKFGPHDL
                     ATETYSVGKKIPYMWGPTMVEVRIWK"
     gene            complement(12134..12628)
                     /locus_tag="VFF28_00560"
     CDS             complement(12134..12628)
                     /locus_tag="VFF28_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44152.1"
                     /translation="MARCILTKKSQVWALDLAVALIIFVSAIVLFYKYSINSMDAGQS
                     ERSSILLDAKLASSYLLSAGYPENWTASDVSVIGLTDGQGRLSYDRLSMFNSIALSDY
                     TRSRRLLSIAHDYNVCFEQGNSTMVIKGIGCVGMAYDGSDVIKITRVVHYNSSIIRMV
                     VKVW"
     gene            complement(12606..12998)
                     /locus_tag="VFF28_00565"
     CDS             complement(12606..12998)
                     /locus_tag="VFF28_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44153.1"
                     /translation="MDKLHLNGQAAMEFLLLAGLLLLVFAVMMGIYSEKMAYSNKQKD
                     ILRGQDLVDKVQKEIAIASRVLDGYQRQFTLPSRLGNKEYTIRIIGKEVIASTDKQDF
                     WRPIPSVVGNVTKGQNNIKRQNGTVYLN"
     gene            complement(12980..13423)
                     /locus_tag="VFF28_00570"
     CDS             complement(12980..13423)
                     /locus_tag="VFF28_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44154.1"
                     /translation="MELSEKKVRCLSRKRGQISLEYMAIVGLTTLIAISLLAISTYYS
                     NQVRETIDSSQIDAIGKQIVDTAESVYYFGPPSKTTLKVFIPKSVYSANISAGQVIFN
                     VRSNHGSSEYFYLSSVPLMGNISSSYGFHYVSVESKEGYVWINST"
BASE COUNT         4060 a         2777 c         2890 g         3907 t
ORIGIN      
        1 aaggcgtttt ttagcgttta ttagccagat gaatttgaca gtttgactgc ttttttcagt
       61 tttagctccg aaagtcaggt agtaggatca aaaagaagct aaaaacgcct tgatttcagg
      121 tgaaaacaac cagttttgac cgaaaatcag tcgaaaaaaa gaagtttaat cgcaactgtc
      181 aaactctatt ttccactcaa aatagtgaag aagtgtcaaa gttgggattt atggaaaaaa
      241 tagcaacact tattttaata ttgtatcgag aggctcagca gaattgaaag aaaaaggatt
      301 gattgagatt atcaaacctg aaagaacata tataaattct ttgatttcaa gttgaactta
      361 gttgaaatta gtaagaacaa gaaaagcttt aaatagtaga tatacctaca atcagtaaaa
      421 tggcaaatta ttatatcaaa gctaatagac aggaatggaa atacaataat cagcatacag
      481 attgggacaa ttttccatta aaccataaac aggattttgc aacatttact aaaaaccagt
      541 tatattggaa aggtcttaaa aagggagata ttattatcgg acatagtagc tacctctctt
      601 ctaatatcaa aggcaaaatt aagtttactc ccctcccaag aatttcagca attgctattg
      661 taaccaatga agaacattat tcaaaaaagt tagatagtga ttcagtgact ctaaaaaaag
      721 tcattgaatt tagtcccatt aaaatatcga tagatataat aaataaaaat aaattatata
      781 atgcagaacc ctttcgatta ggaacaaatc gctgtactat tactaaactc tctgatgaag
      841 aatttgagaa agtaattaag ctaatcgagc aatatcatcc aaatataaaa agagaattaa
      901 aaagtatatg agttttctta ccccttgata ctttgttttg atgtatcggc tttgcctcat
      961 ttgataaaca cacttcttgc ccttgaggta tttccttgaa ggctcacttt gttcgctgga
     1021 ttggagagaa ttccctaaac gatagtctct gatagtctcc ctgcggtcgg attgattgga
     1081 aacttaagtt taactttgcg gatgcaattt tcccccactt tgtaggggaa aagtaactaa
     1141 caggaaccat aggttagcag ttaatacaag aataaacaaa cctggttttc ctatagggaa
     1201 agcctgatat tatcaatatg gtctgctact tcctggcctt tctttgtcaa ggtaagaagc
     1261 tttagccttc cctgcctttc aaagtttatc aggcctgact tctccatctc ctggagtatc
     1321 tttacaacgt gagaatatgt gcaatcgatc tgctttgcaa ggcttgatgc gtaaacttcg
     1381 tctttggaat tcttaaggtt gaccaacatc atggctggct tttctctgaa aaaaacgctg
     1441 aatatcttgt tgtttgcgat attaatcccc ccaacatgtc tattacaata ggtatattct
     1501 actagttact atgaattata tataaatgtt ttggtaaccg tcgataaaat aagttaatga
     1561 ttcctaggtt agcggtgaat tatttggtaa aagtgggatt ttagactata aaattccctt
     1621 gagcgaagcc aacaatgacg agccgaaacg ggggacggcc ctacggggtg gtttcccgag
     1681 tgaaacgagc ggaactagga aaaccgcagt tttccgttgc tcggaggttt tgcgcaaaca
     1741 aaaccgtcga agccattatt gctggctttg agaataacta atcggctcac caatctttta
     1801 ttttctaaac atataaataa tcaggcattt ttcgttttag ttatgataga taaagaggag
     1861 atactgtttc cgtatcctga agtaagagag atacaaagcg acatgataaa agaggtcatg
     1921 gagtgcatag agaatggaga gaatgcgctg atacatgcgc caacgggcct tgggaagact
     1981 gtcgcttcgc ttggacccgc actgtctgtt gctatgaaga aagggctgac aatattcttc
     2041 ttgacctcaa ggcatacaca gcatgtgctt gcgataaata cccttcgcga tataaagaaa
     2101 aagcatggtg tatctgttgt ctgtgctgat atcatcggca agcagcatat gtgcgctctt
     2161 ggtgaagtgg agaatctcta ttcttctgaa tttagggatt actgcaaaaa gatgagagat
     2221 gataagatgt gcgagttcta tatcaataca aagaatacct ctggccgccc tacattaaag
     2281 gcgctatcta tacttgaaga gctaaagcac ttgagcccta tgcatgttga agaggttgtt
     2341 tcgatcgctt caaaggaaaa gctttgccca tatgagatga gcctgcttct tgcaaaagag
     2401 gcaaaggtca tcgttgctga ctattactgt attttcaatc ctgagatacg gcgcagcttt
     2461 atgaacaaga cagagaagga gcttgagact tctatcgtga tagtcgatga ggcgcataac
     2521 cttcccagaa gatgcagaga gcttatgagc cagaaccttt caactgtcat gctgaaacgg
     2581 gcgataaaag aagcaggcaa gtttgggcat tttgaggcta tggacaagct acagggaatg
     2641 aataacttcc ttgaaaagct gactttgacc atagaggaat tcaaggatga aaagctaatc
     2701 acaaagaagg agtttatggc tcttcctaac tatgatgact tggcgggcga gtttgcactt
     2761 atcggagatg aggtaagaga gaaacagaag aagagctata tcgcatccat cgctgtgttt
     2821 cttgataaat ggtcaggtga agacagaggc tttgcacgta tccttcaaag gaaaggagat
     2881 gtggcaaacc ttatctataa gtgccttgat ccttcgttta tttcaaagga agttgttgac
     2941 aatgcacgct ctgtgatatg catgagcgga acacttactc caacttacat gtataaggat
     3001 atactgggct ttgaaaatgc cagggaaatg gtgtttaaga gcccgtttcc atcgaacaac
     3061 aggctcagcc tgataatccc tgagacaact acacgataca gcaagagagg tgctggtgag
     3121 tttgagaaga ttgcagctgt ctgctccaat atagtgaatc tggtacctgg caacagcatg
     3181 gtgttttttc caagctatga ccttcgggat aaggtgtatg cttttcttaa gtgcaacaaa
     3241 gagataatgg tagagaagcc aaggctcact aaggaagaga agaacgagat gctcaatgaa
     3301 ttcaagaagc gcaaagataa aggagcctgc cttcttgcag ttacctctgg ctcttttggt
     3361 gaaggggtag atttacccgg agattttctc aaatgcgttg ttgtggtcgg tctgcctctt
     3421 gagaagcctt cgcttgaggt aagagagctc atcgactact atgatgcaaa gtatgggaaa
     3481 ggatgggaat acgcctatct tatccctgcg atgataatga taatgcagaa tgcaggaaga
     3541 tgcataagaa gcgaaacgga cagaggagtc atagcttttt tagacgagag atacagctgg
     3601 gagcagtact acaagtgctt ccctccagac cataaagtaa agataagcaa gctgtatctt
     3661 gagaagataa aaggattctt tgattaatat acgaaatagt atactagttt ctaaaataga
     3721 aatgtttata tactagtaaa caaattattt atcgaatggt agtattgttt gaccggttta
     3781 acctgccgga agacgtgtat gaagtagtgt ttgcaacaaa gcagcaggtc atagtggcca
     3841 ggctccttgt ggacatgata aaggagaaga acagcgagat aacaaagact gagatgagct
     3901 tttttgcgac acagcttcat gaaggaaagc ttgtgaccga caagatccag gaagagccgt
     3961 atcgcggaaa gagggtaaag atatcataca acaaaaggca gttttacgac aggatactta
     4021 cgcccatgaa gagcatgggg ataatagatt atgatttgta caaaaaaaca tacagactga
     4081 gcgacaagtt caacaaagag atgataagaa taggacttat gtggctgaaa gagctaagaa
     4141 aagaccccta tatgatcgaa aaataatatt caattggctc cctatgataa cctctctttc
     4201 taccaccccc acaatatagg gagccattca tattccttgc tacaggaaaa aagaggtgag
     4261 gaagggtgta aaaaccctca tcataatggg gcaggctgtg gctatctgcc tgccctgtca
     4321 tatttatgaa gattgtaagc aagaggaaag ctatcgagct gttgaagaaa tatgcgaaca
     4381 gtgaagagag ctttaaagct atacttgcac atacacgggc tgttgcaaag cttgctgttg
     4441 agatagcaaa ggatgtccct ggagtggatg ttgagtttat cgagggtgct gcacttctcc
     4501 atgatatagg caggtttaag gcaaaggaaa ctgtcaagca tgggattgct ggcagagata
     4561 tactgctttc tgaagggctt cctgagtatg cttctgtcgc tgaaaggcat cttggggcgg
     4621 gaatctcaaa agaagatata atccagcaga agatagatct cccacttaag gactatgtgc
     4681 ctgtaacaaa ggaagaaaag ataattacac atgctgacaa cttgattgag attaataggg
     4741 tcatttctgt cgagaagacg gttgaaaggt ttaggaagga ggtaggggag gaagctgcgc
     4801 gaagagtcaa gaggcttgct ctggaagtgg aaaatatgcg aaaaattgat gagtaaactt
     4861 taaataatag atttgtttaa gccaataaaa tgaaaaagaa aacaaaagcc aaaccagcta
     4921 gcaaaaaaaa ggtagaaaag ctaaagcctg cgaaagcaag tgtcgatgtt ggttttgaag
     4981 aagttactgt caaatgccca aactgcggca aggtatttcg ggttatcaag agctcagggt
     5041 ttagcatgga aggcatgctt tgccagagat gcgctgctgg cgggggaatc ggccttgatc
     5101 aggatgcaga cttttagtct ttgcgcttct ttttcatccg tttaatatct tcttccttta
     5161 catatttctc atgaataggt atatctgaat catcgatatc cagctctttt cgctttttct
     5221 ttaggttatt gaattcatcc ataagataag ggatgttttt tgagtttata ttatttgcca
     5281 aaaagttatg tgtatagtaa tataggatac ccttgttgct gggttttttc caataatttt
     5341 taaacaacag aattctactg atctataatt ggaggtcaag tcaattctgt gtgctcaaaa
     5401 ccgttaggtg gaattttcta gcatcaaaaa ccacttgctg tttggtgcag gggatgaatt
     5461 gccgattatc ttgaacaaca tttaacctgg aggtgggaag atggatgaca acacaagcga
     5521 gggggacaaa gatgaagaca tatataatgc tcaagaaagg gatgaactta tggaagatga
     5581 ccatataagc cctgtggaac aaggttttat ggaaggggca accgaggacg gccaaggggc
     5641 aaagtgcagg tactgcggca gtgtactgat ggacaacttt gtcgagcaaa ggatagactc
     5701 tgagatctac aggttctgca gcatagagca tgctgaaagg tttaaagaaa gagaaaaaaa
     5761 agaataaaaa atcctgcatg gtatttgtca aaaactccag tattaatgca aagcttcagt
     5821 gaggagtttt tgagcatgct cagaaatttt cttcctatcg ggcactttta gaaaggaaaa
     5881 tttctgacat tttaagcctc aaactgagat atcgccagcc cagctgcggg gtatgtactt
     5941 tagtttgagc aataaagcta tatctttttc ctggctttct tctcctgttt tatcagggta
     6001 tgctttgtct ttatgccaaa accggcaagg gcaaggccga taataagaaa cggcaggcca
     6061 ttgttcgtaa aaccaaaata taagaaggca agcgttatga acaatagtga agaagcaaga
     6121 tacaagtcac ctatcactct ttttcttaga tacattttaa aaataaataa aaaaaattaa
     6181 ttatggaacc cttcctgtta ttgttcctgt tgttgtcttg gcaaatgaac ttacagtgta
     6241 atcaagcttt aagtctgcct ttatcttctc agataatgct gggctgcatg ttaaagtcac
     6301 tgctgtagtg gcgtctgctg aaatagtctg gcctgaaact gtacagatac ctgtgcctcc
     6361 tcctgcagta ttgttcaagg tgactgcaac agaactcata tcatcagtca ggatattctt
     6421 aagatttaat ctaacctggg aagagctgtc tatactaaaa tcgtcgcacc agatgcctga
     6481 gccagctgaa atcacacatt tttccggcag gaatttctcc ggatttaata ctccgaaata
     6541 tgccaaggca ccaatcgcta tgaggacgac caagattgcc caaccataag tcatcaagaa
     6601 ctccatggct gcttgtcctt ttctcattat tttcacctct tttgtttttt tggctaagta
     6661 ggatgttttt agcctaatgt agattacaat tagagcaact catatataaa tgtttttatt
     6721 ttagaaacta gtatactatt tagtatatta tagattttcc taaatattac accctggaga
     6781 gcctatctgg caagaaatcg atgatgcatt gtacttttgc agatgcgcat agtctatctt
     6841 gaaccaactt ggagtctgat ttactctata atcaccattg gatgaaccct gccagtaata
     6901 gtagtctatt atactgctat cttcatcaat attgagcccc tgtttgctta acttcaatag
     6961 atcaaccatg ctctctatgc cataaggaga tgggcccaga tcatttttta gcctcatcaa
     7021 aaagctgggg gctgcaggat ttgcactgta atatgaatgg ttaagatggt ccagcaagtt
     7081 gcttaccttt atctgccccc cagagtcata ggtaaaatta ttttcataag gggtggcatt
     7141 gagattattg gatatcctgc cgtaactgta aatgatgtag agaggatcct caaagccgat
     7201 aaggcttagt gaagcttcta tataagtatc cctttgccag gaggctatag cgcttgtatc
     7261 gttgagtgca aaggaaaagt tagctcctat cctgacatgc catggatcct tttgataaac
     7321 cgtaagattt ccaggggtaa tctcaagacc tatattgaag tttgccgcct catcttctat
     7381 cttttctatc cataaaggca atgtcgactc tgacatgaag taggaacttt ggttttggac
     7441 agtgccattg aaaatcgctt ccctaaaact gttttcaaca tctgagagaa aggtcccatt
     7501 agttatgatg tattcttcaa gtgcgagcaa tgccctatat gatgagatat acagccccct
     7561 ttctgagtcc ctctcaatat ccttgacaaa atcgttcatg gaaagaaccc tcatctccac
     7621 aacccccatc ttctctccca gctgcctgta gttttgagca aggaagacaa aggtgaatat
     7681 gaataggaat gcaatggtca cgacagtgaa atacatgctt ttccttgacc gcatcatgct
     7741 atgctttctc atttccatat ccttacttct accatcgtcg gaccccacat atagggaatc
     7801 ttttttccca cagagtaggt ctctgtggcc aagtcatgcg gcccaaactt gacgaagagc
     7861 ttcccattgt catcgaagtc aagctgcctg aaaagatagc atagtgcatt gtttatcgca
     7921 tcatctgaaa attcagagca atctgtgctg ttgtcaaaga cgcagctgtt tgaaccttca
     7981 taactatccg ggataggcat ctgtacctct gaatcatctt caaatgccag agtccagata
     8041 catccttgcg ctgtctcaaa aatacctgtg tagttaacat caaggtctgc ctcgactgta
     8101 tagataaccc tgtcatcagg agagcctcct gtcgagttat atggattgat gcctgttgat
     8161 atcctcacct gatttgtgcc ttgagagata agttctactg gaatatttat gatataaggg
     8221 tcgccaagag ggtggtaagt gtaaccaaac tctgaaagat tatagaaggc tgtccatgag
     8281 cctgcctgat ttatcaaagc cttgtctgtc cacctgtcac ttgaataaga ggttatctta
     8341 gcatcgctta caatagtgtt ctgaggtatg taaaaggagc cttctgtgat gttgttgtcc
     8401 atccttgctg tctccaaggt tattggaatc ctgttgtaaa cttgaggacc gctatctttt
     8461 gtgtaggtgt aatcgatata agaatcaggg aaaaggacag tagaaatatt acctgtaaca
     8521 ttaactatct gagcttcata ctgcagcttc actatctcct gggcaatctc tttgtatatc
     8581 tcaagtaact cttcagcatt gtttgcactg taatgatggc tgcaatcgtc acagcaagcg
     8641 atatcttcca tcaattcctc atcgcctcca gatccgaaag taaccgaata tatcctgatg
     8701 ccataatcct caaaggcttc acatgctttc tccactgctt cgattcctgc accagtagca
     8761 ttatatgcgc tagtatggcc tatcttggta tttgcctccc cgtcgctcat cactagcatg
     8821 gacatgaacc tgttatatcc agaagtctcg ttattccatc ttagtgtcag attgagattc
     8881 ctatacgacc atgcgtcatt atcgccgctt ttttgatcac agccataggt gtttcctgag
     8941 gttatcctta ctgaaaaggc tgaatcgaaa gcaagggtgg gaatctcaag agttatgttt
     9001 tcccatacag aagagccgga tgtctcagga taatcaagcc ttcctatgta atggccattc
     9061 acataaacac agtcataata tgaaggagca actccataag cttccattgc aagatatgca
     9121 gactttatct cttcttttgc cactcctgta tcaaaaacag ttgtagcaga ctcagcgcca
     9181 tcgtcaagat ttacttgaga aggggaggca taagactgct tgcttgtatt gctaggggat
     9241 tcccagactt ttaacgtaac atcctttagg tcccaatcgt cattgtcacc cgaggtctca
     9301 aagcatcctg aacttgtccc gccagttatt gtaacaaaat ttgtctttgt atccacagag
     9361 taatttaccg atacatcctg cagagcaggg gtaaggttct gctgagagga tgtaagtttt
     9421 gccctccact ggatataacg actggatgat tgagagatat cgcttttaac ctgcactgtt
     9481 gtattatgag gtgtctcttg agagtaaggc tcttcttgaa aataaaagtt atcccatgat
     9541 gaatcaggta ctgctgtgtt tcctgaccgt atatctatac ttagattggt attgttcaaa
     9601 agagcttgcg tccagcttag gccttcccaa cctgcaatac ggcctgcgtc taatacctga
     9661 gaaaggacat agctttctga taggcttaaa cttgatacat taatattatc aaaatctgca
     9721 ttcacagaat aactgctata gataccaatc tttcctgcgg ttatgcctga cgagtcactt
     9781 gtgtcaaaaa tcagctcata gccgctccct ctgtcaaaat aacatttgat gcttgttcct
     9841 tcgacctttg ctttcatctt gtaccacacg cctctgccgg gattaaagct ataatcatat
     9901 cctaggagtg tgtatcctga aggagtatac ctatataatc taaacccata ataaggataa
     9961 ccgtctgcaa actcaaacct atagaagtgg gttgagttat acctaaagta gattcccata
    10021 gggtctgcgt catttatcct tacctttgct tcaactatat aatcctgcca tgattgattt
    10081 ccagctatag accttgagtt atatgcagtc tgcctttggc gatagatacc attctctcct
    10141 acctgccaag tatacccatt ctggtccctg actgcccagt tgcctcctga ctctgaccag
    10201 ccgtttgaat tgccatcgtc aaaattgtca gaaaacaagc cttgctgccc agcaagctgc
    10261 gaggtgttca tcactgcatc ccccggcgca ttatcgagat ctatccttgt gagcgagcag
    10321 gcttcaaaat cctcttttga agtagtcacc cattgtccta tcgaggcatt agatgtgggc
    10381 agccattcct gattgatagg ataagagcag gtgtgccagc ctgagctgcc gtcatcactg
    10441 ggtatgcaca tcctgcctaa gtaatggtcg ttaaaaaaaa cgcaatcctt gtatccaaat
    10501 gttatgtcaa cgcccttgcc aaaaatggtc agagcagcct tgatgaaatt cgaatgattt
    10561 acctctatct ccaagctttt tgttgtagaa acgctgcctg tatccccaag atggacctga
    10621 gaggtatcct gtgcttgctt gactgttgta ccataaagca acttattatc ggtaagaatc
    10681 tgggttgcag cagctatccc gcaggatata caagtatatc cgcaccattc attgtaatag
    10741 tcatcgacgc tgtcttgtag agtagcattg tctgcaatga tatcagtaaa agagcataca
    10801 ggatttagct cttttccata gcctatcatg ccaacagagt ttccctctac tgaaaggatc
    10861 tcatttagga atgttctgtc tgaattttgg gcaaccctgt ctctcctgtc atggcaaggg
    10921 gcataggtgt cacagatgcc aaaagtgcaa tttgtatcca catcacatgc ctccatagag
    10981 cctgtcctgt ctgtaatcaa aacagagtct gaccttccac caaaggcaag cgagacattc
    11041 cttaagccaa gccttaaggg taatgttcgt ttgcctatcc ctgtatagtt caacatgagc
    11101 cgcagctcgc tgtctgttat gtttacggag ttttcgccgt tagaattgta actgtagact
    11161 gtctcgccgc ctaatgccaa aaagatctca tagttgctat cataatgcag ataaacgccc
    11221 atggagtcaa gcgcgccggg tacaaacaaa gatgaataga tattgatgat accatctaca
    11281 cctggaaaag tatacctatc ctgcgagtta tccaccgaga gttctgctga tgtgtgccca
    11341 agcctgatgt aacctcctcc aatatacttt cttgtaccat tgaagccgac cactatagag
    11401 tttgctccgg gctgaaagta ttgcaggtaa gatgagttta tatcccattt tcctgctgcc
    11461 atagaacctg gcggaacata ggcgcctgag tagttgccgt taaaataaag gttgaaactg
    11521 gaacctgcat ctatctccat gtaggcagaa tcgacagagg atagggtaga tggcaggcta
    11581 aattccttgg tcagatttcc gtctccctca tagccgccga agaatatgta ttcgttagca
    11641 aagcgttcat tgatatctgt gagcaatgct cttgcagcaa gaccttctat tgcccttccc
    11701 ctttcaatcc ctgagaccat ctgctttgat gagataagct ggcctgaata agacaggtta
    11761 gaagaaagga caggctctcc gaatccttct atccaaaccc ccattccata atagtcaggg
    11821 accagtcctg acaggatcag gtttagaagc tcagaagcct tatcctcatc tccctcaacc
    11881 tggaagcgaa gtatctgctc aaggatagtc ttgtttacat ctgtcaagtt ttccctttgc
    11941 ctaaaaagtg ctatccctga attatttaac tcatctatcc ttatcgaaga cagcaattgg
    12001 atgacgtctg atgaatagaa tatgttctga gggttttctt cctccttgag gtaaaagagt
    12061 ggggtgagga cgactacggt tatgatgagg atagctgcta tcagcgcatc taagctgaag
    12121 aagaccgctt ttctcaccag accttcacca ccatcctaat gatagaagag ttataatgga
    12181 caacccttgt tatctttatg acatcgcttc catcgtaggc catccctaca cagccgatac
    12241 cttttatgac catcgtcgag ttgccttgtt caaagcaaac gttataatca tgagctatcg
    12301 aaagaagcct cctgctcctt gtataatcag agagggcgat ggaattaaac atacttaatc
    12361 tgtcatagct caggcggccc tgcccgtctg taagaccaat cacggacaca tcagatgctg
    12421 tccaattttc aggatagcct gctgacaaaa gataactaga ggcaagcttt gcatccagca
    12481 ggatgcttga cctttctgac tgacctgcat ccatggagtt tatcgaatat ttgtagaaaa
    12541 ggacaatagc gctgacaaaa ataatcaagg cgaccgctag atcaagcgcc catacctgcg
    12601 attttttagt taagatacac cgtgccattt tgccgtttta tattgttctg accttttgtc
    12661 acattcccta ccacagacgg tatgggcctc caaaagtcct gcttatcggt gcttgctatg
    12721 acctcttttc cgataatcct tatcgtatat tccttattgc ccagccttga aggaagggtg
    12781 aactgccttt gatagccatc aaggaccctt gatgcgatag ctatctcttt ttgaacctta
    12841 tcgacaagat cctgccctct taggatatcc ttttgcttat ttgaatatgc catcttttct
    12901 gaatagatgc ccatcataac cgcaaaaacc aggagaagaa gccctgcaag caaaaggaac
    12961 tccatggctg cctggccgtt taggtggagt ttatccatac ataaccctct tttgattcaa
    13021 ccgacacata atgaaagcca taagatgaag aaatgttgcc cataagaggg acagacgaga
    13081 ggtaaaaata ttctgaagac ccatgattac ttctgacatt aaagatgacc tggcctgcgc
    13141 ttatattggc agagtagaca gatttaggga taaacacctt caaggtcgtc ttggaaggag
    13201 ggccgaagta gtagacggat tctgctgtgt cgacaatctg ttttcctatg gcatctatct
    13261 gacttgaatc gatggtctct cttacctggt ttgaataata tgtggatatg gcaagaagcg
    13321 agatagcaat aagagtagta aggccaacta ttgccatgta ctccaaagaa atctgccctc
    13381 tttttctaga cagacaccta actttcttct ccgatagttc catctacaag cttaaaaatt
    13441 ggctaatccc tatttaaatt tagtgatagc catggccctg aacatgtcaa gaaccgccgg
    13501 gctgaaccgg ggcgtgttct tgagcatgct cagaaatcgc ttgatgtgcc atagacaaag
    13561 catgagcaaa ataggtgcaa taggcgattt ctgacatgtc aagaaccgcc agtcaccgct
    13621 cctttcagtc gcta
//
LOCUS       DASRUK010000013        11107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2020606, whole genome shotgun sequence.
ACCESSION   DASRUK010000013 DASRUK010000000
VERSION     DASRUK010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 11107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11107
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2020606"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            391..963
                     /locus_tag="VFF28_00575"
     CDS             391..963
                     /locus_tag="VFF28_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44155.1"
                     /translation="MKNKQSRWGIVVMFFMAFIMITSVIGFIYGDNTNQPVKYNGIKF
                     IRKADQWSAKIAGKEAVFNYVPQDVESISFGSDAQALIRNKPMLDLTSDPNDLYTEDI
                     ALAEYNLALSLQNFNTYLRQGFVSENAYDLAVITCDNATAAVPVIYFMQSNQTQISAK
                     GNCILAEASSSYDILRVKDRILYSLLGIME"
     gene            966..1670
                     /locus_tag="VFF28_00580"
     CDS             966..1670
                     /locus_tag="VFF28_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44156.1"
                     /translation="MEIEEVDLSGTDEKLKDKRKPISDWILVGSITIIILIGAVIFSV
                     RFFADEEPKTIDDLHKLNLAGKLKDEQGYLYNGYSFVLANGLWYTQVQTIGGENQFDI
                     PLHFGPDDVKDIPVEGALNTTKFDAERSIYITFDPLGQNLNYVALAVGEFDQSIIKAF
                     NKLPVAACDKNETSACQNRPIVVCNSTEKPILYLQQEETPKVIYDDNCIIVQGTGPGI
                     VKSVDRLLLKLYGIMP"
     gene            1671..2258
                     /locus_tag="VFF28_00585"
     CDS             1671..2258
                     /locus_tag="VFF28_00585"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZX44157.1"
                     /translation="MEHYYSEKQTSPLRKKEITSILRQNKLSFITASGTFSLSRIDKG
                     TELLIQSCIIQPSWKILDIGCGYGPVGISIAKAFPQAEIMMADINERALMIAKENSRL
                     NKVNVQIIKSDLYSNIDEKFDTILTNPPQTAGKDVCFKIIEGAKDHLKKEGLLQLVAR
                     HNKGGKTLAKKMEEVFGNVKDTAKEAGYRVYVSKN"
     gene            2339..2821
                     /locus_tag="VFF28_00590"
     CDS             2339..2821
                     /locus_tag="VFF28_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZX44158.1"
                     /translation="MIGSITEFRLLTGRGLQQVIDGEDIVHQVFSSNSVREKLAILKK
                     PQAYLARAIINDINDMYNKWRAIRRSERVYAEDANSRPQAWQSEPFPYDSKEEGLSDR
                     IRPAIDSINARYRKIVTLRHMQHLGINEIARQLRIKPRSVSRYLWEANREMAKYLKLP
                     "
     gene            complement(2808..3164)
                     /locus_tag="VFF28_00595"
     CDS             complement(2808..3164)
                     /locus_tag="VFF28_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44159.1"
                     /translation="MTDLVACLSSGKQTWGHLSKVISGVDWNKVYLITTEEFKSEFKS
                     PKEVQLVVIDTKKTILEMAEDIKKALSGKVCDLEVGLNIISGEGREHMAMISALLGMG
                     VGIRLVVLTKEGVKVI"
     gene            3229..3876
                     /locus_tag="VFF28_00600"
     CDS             3229..3876
                     /locus_tag="VFF28_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44160.1"
                     /translation="MRVFLMILAIVILAIGCQKEQPAVPVQLPPVEAQPEQTVAEPNV
                     TKPLPAQFLQLVDKVKSLDSFSYNYRSPDNKLERQFFVKGSLIKIVLPQMNIEEQGKF
                     YNTIYINASAKRAEAYCIGYSKCEGRTGKIKDLKYDETYIDTPVDWLSKAASAEEKGQ
                     GQIEGRNAIFFQTDKGKFAVEKFYGFLYRVEQEEKAWEFSDASFNSVKNEDVLPQ"
     gene            3873..4295
                     /locus_tag="VFF28_00605"
     CDS             3873..4295
                     /locus_tag="VFF28_00605"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HZX44161.1"
                     /translation="MIRTLLLALPFALGILIFSALLSIIFWLWMLIDCARSDLKEIDK
                     LIWILVIVFFSLVGAFMYLIFVKLNKNYKGIQMYKKAKRLYRSKKERIIAGVCGGIAE
                     YFNVDPSIVRLLWALFALMGGSGILAYIIAWIIIPLEK"
     gene            4297..4860
                     /locus_tag="VFF28_00610"
     CDS             4297..4860
                     /locus_tag="VFF28_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HZX44162.1"
                     /translation="MVFSLQELIDLVVMTFAIGYIFSDFFKKEPEGDYDPLTYYNKPA
                     LWQSIKAGAIIAAPAVVLHELAHKFVAIGFGAQATVQAPYFWYAIVVVLKLMHFPLLF
                     FVGGIVSHTPLPALQSAMVSLSGPLTNLLIWLACLAIVHFRLSKRKYFSYLVPIGRLN
                     MFLFIFNILPLPGFDGFNFLTSLIRAF"
     gene            5764..5907
                     /locus_tag="VFF28_00615"
     CDS             5764..5907
                     /locus_tag="VFF28_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44163.1"
                     /translation="MGKKPVSATIDEELISWIEKKVKDKNRFRNKSHLIEIALERLKS
                     EDK"
     gene            6164..9946
                     /locus_tag="VFF28_00620"
     CDS             6164..9946
                     /locus_tag="VFF28_00620"
                     /inference="COORDINATES: protein motif:HMM:NF026240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HZX44164.1"
                     /translation="MPLFDKTPFSYDILREGEEIILQINCETYTKMPSIEDDPLTMQK
                     TVDILNEVGAITKIAFFQRRRIEYDFSQVNLLREIAVIYKKIIKQRETLGYNAFVYDP
                     QCTKCVTGWYTEIRNLITDTLRSDPISTYVELRRIIRDETIKRDKATDPRCVRCEQKY
                     IDLLSGISGMLENTQILAMAKPHLAGFKVGTRDLYRMIFKPLIKPDFLFTKLMSKFPE
                     EGEELDSYMVNETEVTIFKVPDTIQYLYHISPPEFRLSEDQYEILDSARKIMTEHKPK
                     RSEFVDPERMRSVFFNIGHDLIEELADFKKIRLKEKEIDELTKILVRYTVGFGLIEVL
                     LEDENIQDITINSPMGEIPIFLVHAEFGDCRTNIIPTVTEADSWASKLRMISGRPLDE
                     ANPILDAELILPDARSRVAVISSPLNPSGLAYAFRRHRDKPWTLPLFIHNRMLNPLGA
                     GLLSFIIDGSRTMLVAGTRSAGKTSLLGGILTEIMRKFRVITIEDTLELPGESLRKLG
                     YNIQQMKVAAAMTKSTSEVSAEEGIRTTLRMGDSCLIVGEVRSSLRGDQEVLVIQDGL
                     TKRVAIKSLENLDLKSVYLPTLTSQNTMEIKPITGFVKHPKRNRLIKLTTKTGRSVIV
                     TPDHSVFTHVNFKIAAINTDKLKPGDPIIIPSKLPNNFNDIDHIDLLKIFKENYRLEH
                     AEPYLRKAIKILGWAKASKICGVADIYRYLLSTQKTRIPIGSFLKLMGEAKIKYNISD
                     LRITKGSANSIPAKFPINENVMRLIGYYLAEGNIGKDKIQVTNSKPSIIEDVKYICEK
                     EFGLNVSQRKITGYGSSVQMFIISKPLRDLLIYLGCGKTSFYKRIPEFVYGLNKRKIC
                     ALLKGMYSGDGSISSSVAAGNMIRYFSTSKKLIEDVSFALLSLGIVTRIGKRAPGKDG
                     KKILFVAEIKQRKYVECFLENIGFTHKKPSILMRSFAHSKDDSVSFDPKELENHIKLI
                     RKYRHLRRTRSCSKEYLRKITEEVKCSDELYNFAHGDFFIDKVRSIETIDLKEPENVY
                     DLEVISTQRFIGGFGGILLHNTEARSLYEAMRVGALANVVAGTIHGDSPYGVFDRVVN
                     DLNVPRTSFKATDIIVVANPIRSADGMHRWRRITQITEVRKHWDQDPLLENGFCDLMK
                     YDSKSDELEPSQDMINGESEILKSIAGSVKEWAGNWDAVWDNILLRTKIKEALVNIAE
                     KQNDLSLLEAPFVIQSNDEFHRISDTVRDEVGSLDSKRIFFEWNEWLKRSVKKKHL"
     gene            9955..>11107
                     /locus_tag="VFF28_00625"
     CDS             9955..>11107
                     /locus_tag="VFF28_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44165.1"
                     /translation="MNSLVSRYREKLEKQFGPVVEENLRKPMPTREYTQFKEEYLPGH
                     LSYYEKLCNLSEKLVRIDPDPSRAAELKESIEICHLNITPPGATSFAMLTPIFFVVVS
                     SLLSILFLKSFFFVLFFIVLSVSIIKPLGNLPIFFANTWRLKASNQMVLCIFYVVTYM
                     RHTSNLELAIKFASDHIAAPLSLDLKKIIWDVETEKYSTIKESLEAYLSTWRKWNLEF
                     IEAFHLIESSLYEGDEKSRVASLDKSLDVILTETYERMLHYAQNLKSPITILHMLGII
                     LPILGLVILPLVVSFLAEVKWYYISLLYNIALPILVFYLGKTILSKRPTGYGETDISE
                     LPEYKNCSGLTLTFSKKKVQIKPSTVAAIIFGVLFLIGIIPPLLHIIIPD"
BASE COUNT         3603 a         2195 c         2318 g         2991 t
ORIGIN      
        1 ttgtaaagga attcaagcgc aggaccagca ttgaaagggt aaacagtcac tcaaaaattt
       61 atacacagat tagtctgcta caaaataaga ttaagaagag tcatacaatc aagaagacaa
      121 cggcagaaac gatgattatt acttccttta ttttggagca attcaagttg ttagccagaa
      181 tcaatgccaa acttgcgcaa tcgacgctaa gccaatattt tcagtgaatt tatcaattgc
      241 ctcagataat aactaacagt tctatccacg ggctagtggc aagtcattat ttaaacttcc
      301 tttttttgct tgactaaaca aacgaaaaat aacccgatag gtaaaaaacg aaacaaacct
      361 ttaaatatta acaagtactc catcgcttgc atgaaaaaca agcaatcaag atgggggata
      421 gtggttatgt tctttatggc attcatcatg ataacctctg tcatcgggtt tatctatgga
      481 gataacacta accagcctgt taagtataac ggcataaagt tcataagaaa agcagaccaa
      541 tggtctgcaa agattgcagg caaggaagct gtctttaact atgtccccca ggatgttgag
      601 tcgataagct ttggttctga tgcacaggca ctgataagaa acaagcctat gcttgaccta
      661 acctcagacc caaatgatct ttatacagag gatatagctc ttgcagaata taatcttgca
      721 ctttcccttc aaaattttaa tacttacctt aggcagggat ttgtctcaga aaatgcctat
      781 gatctcgctg tgataacatg cgataatgcg accgcagctg ttccagtcat ctactttatg
      841 cagtcaaacc agactcagat ttccgcaaaa ggaaactgta tactggcaga agcaagctca
      901 agttatgata tcttgagagt caaggatagg attctctatt ctttgcttgg gataatggaa
      961 tgattatgga aattgaagaa gtagacctta gcggcactga cgaaaagctc aaagacaaaa
     1021 gaaagcccat atcagattgg atactggtag gttcgataac catcatcata ctgataggag
     1081 cagtcatctt cagcgtaaga ttctttgctg acgaagagcc aaagacaata gatgatcttc
     1141 acaagctaaa tcttgcaggc aaactaaaag acgagcaagg ctatctttac aatgggtata
     1201 gctttgtttt ggcaaacggc ctatggtata ctcaggtaca gacgataggc ggtgagaacc
     1261 agttcgacat ccctcttcat tttgggccag atgatgtaaa agatatccct gtggaaggcg
     1321 ctcttaatac gactaagttc gacgcagaaa gatcgatata catcacattt gaccctctag
     1381 gccaaaacct taactatgtt gctcttgcag taggtgagtt tgaccaaagc ataataaaag
     1441 ccttcaataa gttgccagtt gcagcctgcg ataagaatga gacatctgcc tgccagaaca
     1501 ggcctattgt tgtatgcaac agcacagaaa agcccattct ctatctccag caggaagaga
     1561 cgcctaaggt catatatgac gataactgca tcatcgtcca aggcactggc ccgggtatcg
     1621 tcaagtcggt cgacaggctg ctgctaaaac tgtatggtat aatgccctaa atggaacatt
     1681 actattctga aaagcagaca tctcctctaa gaaaaaagga gataacctcg atactaaggc
     1741 aaaacaagct ttctttcatt acagcatcag gcactttctc tctatcccgg atagataaag
     1801 gtacagaact tcttatccaa tcctgcataa tacaaccttc ttggaaaatc ctggacatcg
     1861 gctgcggtta tggccctgta ggcataagta tagcaaaagc atttccgcag gcagaaatta
     1921 tgatggcaga cataaacgaa agagccttga tgattgcaaa ggaaaactca aggctaaaca
     1981 aggtaaacgt ccagataata aagtcagacc tatactccaa catagatgag aaatttgaca
     2041 caatccttac taatccgccg cagacagcag gaaaagatgt ttgtttcaag atcatagagg
     2101 gagcaaaaga ccacttgaaa aaagaaggcc ttcttcagct cgtagcaagg cacaataaag
     2161 gcggaaagac cctggcaaaa aagatggagg aggtctttgg caatgtcaag gatacagcaa
     2221 aagaggcagg atatagggtc tacgtctcca aaaactgagt tatcttttta taatatcttc
     2281 tattatttcc accttatgca atcaaaatca atattccaag tcagaggatt ttgagaaaat
     2341 gatcggctca ataactgaat ttcgtttact cacgggcaga ggactgcaac aggttattga
     2401 tggagaggat attgtccatc aggttttttc gtccaattct gttagggaaa agctcgccat
     2461 tctcaaaaaa ccacaagcat acctggccag agcaataata aacgatatta atgatatgta
     2521 taataaatgg cgggcgatca gaaggtcaga gagggtatat gcagaggatg caaatagcag
     2581 gccacaggca tggcaatcag agcccttccc ttacgactca aaggaggaag gtctgtcaga
     2641 taggatacga ccagccattg attctatcaa cgccaggtat cgcaagattg ttacccttag
     2701 acatatgcag catttgggaa taaatgagat agcaagacag ctccgaatca agccgagatc
     2761 agttagtagg tatctttggg aagcaaacag ggaaatggca aaatacctta aattacctta
     2821 accccttcct ttgtcaggac aacaagcctt attccgactc ccatcccaag cagtgcagag
     2881 atcattgcca tatgctctct tccttcgccg gatatgatgt taagcccgac ctcaaggtcg
     2941 cagacctttc ctgagagtgc tttttttatg tcttcagcca tctcaaggat ggtcttcttt
     3001 gtatcgatga ccacaagctg aacttccttg gggctcttaa actcgctctt gaactcctct
     3061 gtggttatca gatagacctt gttccagtca acgcccgata tcacctttga aagatgcccc
     3121 caggtttgtt tgcctgaaga taaacaagct acaagatcag tcatacctgg cgctaatttg
     3181 gcaaaattta aatagttttc ttgctagctg gaagatagag gtgattttat gagagttttc
     3241 ttgatgatac tagcaatagt tatcttggca ataggttgcc aaaaagaaca gccagctgtg
     3301 cctgttcaat tacctcctgt tgaagcacag ccagaacaaa ccgtagctga gccaaatgtt
     3361 acaaaacctc tgcctgctca attcctccaa ttggtcgaca aggtaaaatc cctcgatagc
     3421 ttttcatata actacaggag cccagataat aagcttgagc gtcaattctt tgtaaaaggc
     3481 agcctgataa agatagtcct gccccagatg aatatagaag agcaaggaaa attctataac
     3541 actatctata taaacgcctc agcaaagagg gcagaagcat actgcattgg ctattccaag
     3601 tgtgaaggaa gaacaggaaa gataaaggat ttaaaatacg atgaaaccta catagatacc
     3661 ccggtcgatt ggctgtctaa ggcagcatcc gcagaagaaa aaggccaggg ccagatagaa
     3721 ggaagaaacg ccatcttttt tcagacagac aaaggaaaat ttgcagtaga aaaattctat
     3781 ggtttcctct acagggtcga gcaggaagaa aaagcatggg aattctcaga cgcttccttt
     3841 aactcagtaa aaaatgagga tgtattgcct caatgataag gacattattg cttgcacttc
     3901 cctttgcact gggcattttg atattttcag cgcttcttag catcatcttt tggctttgga
     3961 tgcttatcga ttgtgcaaga agcgacctaa aggaaataga taagcttata tggatattag
     4021 tcattgtctt cttcagcttg gtaggtgcgt tcatgtacct tatctttgtc aagctaaata
     4081 aaaactataa ggggattcaa atgtataaaa aagcaaaaag gttgtacagg agcaaaaaag
     4141 aaaggataat tgcaggagta tgtgggggca ttgcagaata cttcaacgtc gatccttcca
     4201 tagtcaggct tctttgggcc ttattcgcct tgatgggcgg ctcagggatt cttgcatata
     4261 tcatcgcctg gataatcata cctttagaga aataatatgg ttttttctct ccaggaactc
     4321 atagaccttg tagtgatgac ctttgcgata ggttatatct tctctgattt tttcaaaaaa
     4381 gagccagaag gagactatga tccattaacc tattataata agcctgcatt gtggcagagt
     4441 ataaaggcag gagcgattat tgcagccccg gcagttgttc tccatgagct tgcccataaa
     4501 tttgtggcca ttggatttgg agctcaagcg acagtccagg cgccatattt ctggtatgct
     4561 atagttgtcg tactaaagct catgcacttc ccattgcttt tctttgtagg aggcatagtc
     4621 agccacactc ctcttcctgc cttgcagtct gcaatggtat ctttatcagg cccgttgacc
     4681 aatctcctga tatggctagc ctgccttgcg atagttcatt tcaggctctc aaagagaaaa
     4741 tattttagtt atcttgtgcc tatcggcagg ctgaacatgt ttctgtttat cttcaacata
     4801 ctgccactgc caggatttga cggattcaat ttcttgactt ctttgatcag ggctttttga
     4861 ttgctcaaga ctagggctca taccccgtag cttgctacgg cttaataacc cagtttgagg
     4921 cttaaaatgc cagaaatttt ccttgctaaa agtgctcgat aggaagaaaa tttctgagca
     4981 tgctcaaaaa ctcctcactg aagctttgca ttaatactgg agtttttgac aaataccccg
     5041 cagctaggct gcggttttgg atttttttat ttaggaattg gatgatacat cttaagctag
     5101 gagcctatac gcaattggag caactttttt cacatctcaa cttttcatca tcctttctaa
     5161 ttaattggca aactgactta tttttcaatt cataagcaaa gctgctatag cacaaatccc
     5221 tataatacca tgattcatta atgctatcac aaaaatcaat acttccttcc ttcttcctaa
     5281 aatgttccag gcacatttga gtaaacgtaa aatcgtcttt gatcaagtaa cagacagaca
     5341 ggttttttgt ctttattgca aattcttgca tgcaatagcc tttatagctt atttcaggtg
     5401 catcacagtc agaaagttgc tttggattcg tttttttata tacactaaaa tcatccaaag
     5461 gaaatattga ccagaaaata gatgaaaaaa tgataaaagt gcaaattatt aatgatcgca
     5521 cctttctgga tgaatatttt tttcgaataa aatagtaaat gatgaatgat attatcaaag
     5581 gaactgcaaa gattcttatt aatccatata tcagcaccaa taacaaaaat aatcctaacc
     5641 gttcaatcat actttatctt catttagcta tacctataaa aatatttagg gcgggggtta
     5701 tatgataaaa tatatttaat catagaaagt attataagct atatattata tgagttagcc
     5761 ctgatgggaa aaaagcctgt ttcagcgacc attgacgaag agctgatcag ctggatagag
     5821 aaaaaagtca aggataagaa tagattcagg aacaagtcgc acctgataga gatagcgctg
     5881 gagagattaa agagcgagga taaataagaa accagcaaag gccaaccata tggcgagacg
     5941 aaaaccaaca atgatgcaaa cgaggggagg gttgttccgt cggaaagttg ccgagacaaa
     6001 aggtcgagcg caactaagaa aagcttgctt ttctgttccc ctcgtagggc aaagcgaagc
     6061 tttgaccgcc tgcatcatta ttgttggttt tgtttaatgc aaccgtcgaa gccattattg
     6121 ttggcaagtt cacatattta actaataaaa aaagaggtaa acaatgccat tgttcgacaa
     6181 aactccattt tcctacgata tacttaggga aggagaagag ataattcttc agattaattg
     6241 cgagacttat acaaagatgc cgtctatcga agatgatccc ctcaccatgc aaaagaccgt
     6301 tgacattctt aatgaggtag gagcgataac taagattgct tttttccaaa gaaggaggat
     6361 agagtatgat ttctcccagg tcaacctttt aagggaaata gcggtcatct ataaaaagat
     6421 tataaagcag agggagacct tgggctataa cgcctttgtc tatgatcctc aatgcacaaa
     6481 atgcgtcact ggttggtata ccgagataag aaacctgata acagataccc taaggtcaga
     6541 ccctatcagc acttatgttg agctgagaag gataatcagg gacgagacaa taaagcgcga
     6601 caaggcaacc gacccaagat gcgtccgctg tgagcagaaa tatattgacc ttttatcagg
     6661 catatcagga atgcttgaaa atactcagat cttggccatg gcaaagccgc accttgcagg
     6721 attcaaagtc ggcacaagag acctctaccg gatgatattt aagcccctca tcaagcctga
     6781 ttttctcttc accaagctca tgtctaagtt tcctgaggaa ggagaagagc ttgactccta
     6841 catggtcaat gagacagagg tgaccatctt caaggtacct gacactatcc aatatctcta
     6901 ccatataagc cctcctgaat ttaggcttag cgaagaccaa tacgagatat tggattcagc
     6961 aagaaagatt atgaccgagc acaagccaaa gcgctcagag tttgtagacc cagaaaggat
     7021 gcgaagtgtt tttttcaata tcggccatga ccttattgaa gagcttgctg actttaagaa
     7081 gataaggcta aaagaaaagg agatagatga gctcaccaaa atcttggtta gatacacagt
     7141 tggctttgga cttatcgaag tcttgttgga agatgaaaat atacaagaca tcactataaa
     7201 ctcgccgatg ggagaaattc ccatatttct tgtccatgca gaatttggtg attgcaggac
     7261 aaatatcatc cctacagtca cagaggcaga ctcctgggca tcaaagctaa ggatgatctc
     7321 aggacggccc ttagacgaag caaatccgat tttggacgca gagcttatct tgccggatgc
     7381 aaggtctagg gtagcagtca tctcatctcc attaaatcct tcaggccttg cttatgcgtt
     7441 cagaaggcac agggataaac catggaccct tccgttgttc atccataata ggatgcttaa
     7501 tccactggga gcagggctcc taagttttat cattgatggc tcaaggacca tgcttgtagc
     7561 aggcacaaga tcagcaggaa agacctcatt gttaggcggt attttgacag agataatgag
     7621 aaaattcagg gtaatcacaa tagaagatac cctggagctt ccaggagaat ccctaagaaa
     7681 attaggctat aacatccagc agatgaaagt tgcagcagcg atgaccaaat caacctcaga
     7741 agtctcagca gaagaaggta taaggacaac actgcgtatg ggagactcat gcctcatagt
     7801 aggagaagtg agaagttcat tgagggggga tcaagaagta ttagtgatcc aggatggatt
     7861 gactaaaagg gtcgctatca aaagccttga aaatttagat ctaaagtctg tttaccttcc
     7921 aacattaact agtcaaaata caatggaaat aaaaccgatt accggcttcg taaagcatcc
     7981 taagagaaat aggctgatca aacttacaac aaagactgga agatcagtga tagtaactcc
     8041 cgaccatagt gtttttaccc atgttaattt taagattgcc gcaataaata cagataagct
     8101 aaaaccaggg gaccctataa taattccttc aaaattgcct aataacttta acgatataga
     8161 tcatatagat cttttgaaga tttttaaaga aaattatagg ttggagcatg cagaacccta
     8221 tctaagaaaa gccattaaaa tccttggatg ggcaaaagcc agcaaaatat gcggggtcgc
     8281 tgatatttat cgttatttgt tgtcaaccca aaaaacaagg atccctatag ggtcattttt
     8341 aaaactcatg ggggaagcta aaataaaata taatatttca gatttgagga taactaaggg
     8401 gtctgcaaac agcatccctg caaaattccc tatcaatgaa aacgtgatgc ggttaatagg
     8461 ttattatttg gctgaaggca atatcggaaa ggataagatc caggtaacca attcaaagcc
     8521 tagcattata gaagatgtaa aatatatctg cgaaaaagaa tttgggttga atgtctcgca
     8581 gagaaaaatc acaggttatg gaagttctgt tcaaatgttt atcatatcca agccattaag
     8641 ggatttattg atataccttg gttgtggaaa gaccagtttt tataagagga tccctgaatt
     8701 tgtctatggg ttaaataaaa gaaagatatg tgctttgctt aaagggatgt acagcggaga
     8761 cggttctatt tcctcaagtg tagctgcagg aaatatgatc aggtattttt caacctcaaa
     8821 aaaattaatt gaggatgttt ccttcgcttt gttatcttta ggtatcgtaa cgaggatagg
     8881 gaaaagagcg ccaggaaaag atggaaaaaa gatattgttt gtagctgaaa taaaacaaag
     8941 gaagtacgtg gaatgcttcc tggaaaatat tgggtttacg cataaaaagc cgagtatatt
     9001 aatgagatca tttgcacata gcaaggatga ttcagtgtct tttgatccaa aagagctgga
     9061 aaatcacatt aagcttataa gaaaatatcg gcatctaaga agaacaagat cttgctcaaa
     9121 agagtacctt aggaaaatta cagaagaggt taagtgcagc gacgaattat acaactttgc
     9181 ccatggagat ttcttcatcg acaaggtaag atcgatcgag accatagacc ttaaggaacc
     9241 ggaaaacgta tatgatcttg aagttatatc gacccaaaga ttcattggag gattcggggg
     9301 tatattgctg cataatacag aagctcgctc actttatgaa gccatgcgtg ttggcgctct
     9361 tgcaaatgtt gtcgcaggaa ccattcacgg tgattctccc tatggagtgt ttgacagggt
     9421 agtcaacgac ctaaacgtcc caaggacaag ctttaaggcg acagatatta ttgtggtcgc
     9481 aaatccgata aggtcagcag atggcatgca caggtggaga aggataactc agataacaga
     9541 ggtaagaaag cattgggacc aggatccact ccttgaaaac ggcttctgtg atctgatgaa
     9601 atatgacagt aaaagtgacg agcttgagcc atctcaggat atgataaatg gagaatcaga
     9661 gatactaaaa tcaattgcag gctcagtaaa ggaatgggca ggcaattggg atgctgtctg
     9721 ggacaatatc cttctcagaa ccaagataaa agaggccttg gtaaacatcg cagaaaagca
     9781 aaatgacctt tcattgcttg aggcgccctt tgtcatacaa tcaaatgacg agttccacag
     9841 gatttcagac acggtgaggg acgaggtagg aagccttgac tcaaaaagga tattctttga
     9901 atggaacgaa tggctcaaaa ggagtgtaaa aaagaagcat ctatgaccga agatatgaat
     9961 agcctggtct caagatacag ggaaaagctt gagaagcagt ttggccctgt tgttgaggaa
    10021 aacttaagaa agcccatgcc tacaagggaa tatactcaat ttaaggaaga gtaccttcca
    10081 ggccatctca gctattatga aaagctctgc aacctgtcag aaaagctggt aaggattgat
    10141 cctgacccga gcagggcagc agagctaaag gaatcgatag agatctgcca ccttaatata
    10201 actccaccag gggctacttc ctttgccatg ctcactccta tcttttttgt agttgtttca
    10261 tctttgctct ctatattatt cctgaaatct tttttctttg tcttattctt cattgttctc
    10321 agcgtatcga taataaagcc tcttggaaac ctccctatct tttttgcaaa tacctggcgt
    10381 ctcaaggcat ctaaccagat ggttttatgc atattctacg tcgttaccta tatgaggcat
    10441 acctctaatc tcgagcttgc cattaagttt gcatcagacc atatagcagc tcctcttagc
    10501 ctggacttaa aaaagattat atgggacgta gagacagaaa aatattcgac cataaaagaa
    10561 tctcttgaag cttacttgtc tacatggaga aaatggaacc ttgaatttat cgaggcattc
    10621 catttgatag aatcctcatt gtatgaaggt gatgaaaaat caagggtggc aagccttgat
    10681 aagtccctcg atgttatcct gacagagaca tacgaaagga tgcttcacta cgctcagaac
    10741 ctaaagagcc cgataactat ccttcatatg cttggtataa tcctgcctat cctggggttg
    10801 gtcatccttc cactggtcgt aagcttcctt gccgaggtaa aatggtacta tatctctttg
    10861 ttgtacaaca tagccctccc catcctcgtc ttttaccttg gaaagaccat cctgtcaaaa
    10921 aggccgacag gttatggaga aactgatata agcgagcttc ctgagtataa gaattgcagc
    10981 gggctcacct taacttttag caagaaaaag gtgcagataa agccctctac agttgcagcc
    11041 atcatatttg gcgttttatt cttgataggg ataattcctc cccttcttca tatcatcatc
    11101 ccagatg
//
LOCUS       DASRUK010000014         8334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_352463, whole genome shotgun sequence.
ACCESSION   DASRUK010000014 DASRUK010000000
VERSION     DASRUK010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8334
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352463"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..719)
                     /locus_tag="VFF28_00630"
     CDS             complement(96..719)
                     /locus_tag="VFF28_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZX44166.1"
                     /translation="MKAIFCSDLHGNEAYYDRLLSFAKQEMADAIIIGGDLCPHLKID
                     IIDAILGQKQFIEGFLASFSERCVSKGIALYLIMGNDDFRANYPLLKKFNNLHNLVKD
                     LGRWNITGYSFVPPHPFLLKDWEKPDFDYLELTEHPREVRTAPRELGTIREDLDKLGK
                     LCNPQETIYVMHSPPYSTLLDKTYSGQHVGSRAQLFEPITQNNHSKQ"
     gene            1115..2788
                     /locus_tag="VFF28_00635"
     CDS             1115..2788
                     /locus_tag="VFF28_00635"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF003169.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HZX44167.1"
                     /translation="MDLIRKYVLQNSIKFSGKASAGAVIGKIIAEKPELKEKIKEIAA
                     QVNKEIKEVAKIPLKEQEEELKRVAPELLEEKKQESKRVLPELKDPKNLVMRFEPSPS
                     GPLHIGHAYVLSLNSEYCRKYNGKLILRISDTNPGNICDPAYEMIPKDAQWVTKSNVK
                     EVHIQSDRLATYYNYMEQLINLGKAYVCTCEPEGYKKLLHESIACPCRELPVEVQMKR
                     WKRMFDEYKQGEAVVRIKTDLNHKNPAMRDFPVFRISELEHARQDKRYRVWPLMNMAV
                     SVDDIEMKVTHAIRAKDHYDNAERQKYIYSYLNKKFPQTLFVGRINFEGMPVSCSKTR
                     PLIEDGTYIGWDDIKLPFLAALKRRGYQPGAFIKYAIDVGLSLNDKTVTRDEFFKTIN
                     AFNKDIIDPVSNRYFFIWNPVEITIEKAPSHEVELDLHPDHKKGGRKFSTRSSFYITG
                     EDFKAIKGGKLYRLMDCLNFQKKGSKFIFDSLDYQQFKEKGDRIIHWLPKEKLPEVEV
                     FMPDGVIHKGIGEKALTDMKVGDICQLERLGFCRLDKKEKKLSFWYAHK"
     gene            2798..2980
                     /locus_tag="VFF28_00640"
     CDS             2798..2980
                     /locus_tag="VFF28_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1931 domain-containing protein"
                     /protein_id="HZX44168.1"
                     /translation="MGKYLVVRSNIKDYAKYNERPLNVSEDFYEKLNLKVAEIIKESC
                     RRAKENSRNTVMGKDI"
     gene            complement(2977..3522)
                     /locus_tag="VFF28_00645"
     CDS             complement(2977..3522)
                     /locus_tag="VFF28_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44169.1"
                     /translation="MSDTLSIDPGRLKRFIKHACVIAKKQSDREKARKELQLQMQKLR
                     KFASRKGDIEAELVELNKKINILFEKEVQLLGIEQKESVLTRELVNGVNTNKDKIKEI
                     YSIVDNLQTKLDGWVKVKTQRERKIEELEHKIRHSHKENKAVLAAKLNKLEQSYMELC
                     RKGISSTQLEMRIGAIKARLS"
     gene            complement(3519..4157)
                     /locus_tag="VFF28_00650"
     CDS             complement(3519..4157)
                     /locus_tag="VFF28_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44170.1"
                     /translation="MTDEGSYEIMPYKEIVELKKQISELQLRAGDTSSKELLLSMAAL
                     TKSVNELLKLFSSAAEEMKLEEKTEGELSQKIGPLIEKVSNLEQQNRTIAEGLVAVAD
                     MVKQIKGDKRMAPRQSMNEEDDEDTEREPAFFSKAKAQRRQSGPGFAPPPVPPMMGPG
                     PMAPPPMQGMMPPPMPPGMGQLRSPAPDFGELPPLPPFEEEPRKKGLLGIFK"
     gene            complement(4263..5894)
                     /gene="thsB"
                     /locus_tag="VFF28_00655"
     CDS             complement(4263..5894)
                     /gene="thsB"
                     /locus_tag="VFF28_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit beta"
                     /protein_id="HZX44171.1"
                     /translation="MSKEIQPIFILPEGTQRTTGRNAQRTNIMAAKLVAETVRTTLGP
                     KGMDKMIVDSMGDVTVTNDGVTILEEMQIEHPSAKMIVEVAKTQEDEVGDGTTTAVVI
                     AGELLKNAEDLLDQNVHPTIIARGYRLAEGKAQEILNKIAEDVSEKDTDILRKIAMTA
                     MTGKGAEHSKEKLADIAVEAVRSVAEQQNDRLSVDKENIKLEKKVGASVEDSELVRGI
                     ILDKEKVHGGMPRVVKSARIALIDSAVEIKNTEIDAKIQITDPSQMQAFIDQEETMLR
                     NKVEKIAASGANVLICQKGIDDIAQHFLAKKGIYAVRRAKQSDMVALAKATGGNVVTN
                     LDDLSKEDLGLAGTVEEVKVGDEQMTYVKDCKNPKAVTILIRGGTEHVVDEVKRAMED
                     SIGDISAALKDGKVVGGAGAPEVEVAKQLRKFADSLSGREQLAVMKFADAMEIIPRTL
                     AENAGIDPIDILTELKAAHDKGEKWSGIDVFKGKAVDSWKKGVIEPLKIKTQAISSAA
                     EVAVMILRIDDVIAGSGSSKGPAMPPGGMPPGMDM"
     gene            6108..6569
                     /locus_tag="VFF28_00660"
     CDS             6108..6569
                     /locus_tag="VFF28_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44172.1"
                     /translation="MNKLYISQAIPQIAYLRPKNARVLATHMLTRWQHEPDDPAPDLT
                     LIIREDSTLFYFRQSFAESQAYIFSHQVPYDEFLGDLSDLFRTQGEETDIIPATYRVR
                     QSIEELVTPYESAHIEEISPLLVTPPPEIASPTLKQIIRLRYYPTQTLLET"
     gene            complement(6544..7410)
                     /locus_tag="VFF28_00665"
     CDS             complement(6544..7410)
                     /locus_tag="VFF28_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZX44173.1"
                     /translation="MKESLVEKIGIFLLRKGFTVKTLNRTAFDIVARRDTTILLIKIL
                     FDANAVSEEFTKSMHNVAAYVGGSPLIIADKAGDKLEDNVVYLRFGIFTLNFRTLCNS
                     IDSKLPFAIRDHSGVNAHLIGTKLRKVREEEGISLNDLARKVGVSKSMVQKYEAGESR
                     ISINKALRLYQLLGHSLFEKIDIFGHDKNIMPVGKGDISRKFSDMGFSATEAEKVPFD
                     IIAKKEKELILTKVGDGQQQGLDSLSKLVDADRLAIYLKKKPKNVPAMTKKEFMEFEK
                     AEELIKFLKEFE"
     gene            complement(7444..7650)
                     /locus_tag="VFF28_00670"
     CDS             complement(7444..7650)
                     /locus_tag="VFF28_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44174.1"
                     /translation="MNRNTWRNHRRDLCSGKAYRYLSCLYGGAVAYVGATLAGLSRAI
                     LGSISGMLAVAVPAAFYRACCRKT"
     gene            complement(7684..7917)
                     /locus_tag="VFF28_00675"
     CDS             complement(7684..7917)
                     /locus_tag="VFF28_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44175.1"
                     /translation="MGLEAILGHASRAAYAIKEFYMNFYATYSPLATYGALIVTGAAV
                     AAATILGARIIIGFVEPITKYFGAKPKKEAKPG"
     gene            8035..8232
                     /locus_tag="VFF28_00680"
     CDS             8035..8232
                     /locus_tag="VFF28_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44176.1"
                     /translation="MNKKMRDEMEMGKESMGTKRGMSESHGPLTKKPSPIGISKSLKG
                     IDFPKNKEEVKLSSLTAEASF"
BASE COUNT         2224 a         1952 c         1681 g         2477 t
ORIGIN      
        1 gtccctacgg gatgtctcgt aggttatttt gcacagagca aaataaccgt ctcatcccat
       61 gttgttgtta tttttacatt tgtcataata cagaattact gtttggaatg attgttttgg
      121 gttattggtt caaacaactg agctctgctt ccaacgtgtt ggccgctata tgtcttgtca
      181 agcagtgttg aataaggagg gctgtgcatg acatagattg tttcctgggg gttgcatagc
      241 ttcccaagct tatcaaggtc ttctcttatc gtcccaagtt ccctgggagc ggtccttact
      301 tccctggggt gttcagtcag ctccagataa tcaaaatcag gtttctccca atcctttaga
      361 agaaaagggt gcggcggcac aaagctgtat cccgtaatat tccatctgcc tagatccttg
      421 actaggttgt gtagattatt aaattttttc aacaatggat aatttgccct gaaatcatca
      481 tttcccatga tcaggtatag tgcgatgccc ttactgacgc accgctcaga aaaagaagca
      541 aggaatcctt cgataaattg tttttgcccc agtattgcat caatgatatc gatcttaaga
      601 tgggggcaaa gatccccgcc gattatgata gcatctgcca tctcctgttt ggcaaaggaa
      661 agcagtctat catagtatgc ttcattccca tgtaggtcag aacagaatat tgctttcata
      721 aaataaggtg gacctgccgg aacttccaac tccctgcatc cacagagaat ctttgaatcc
      781 ggatatcacc ctagccaagg gcgtattcta ccgggttgaa tcacaggccc atgaaaatca
      841 aggttctgtg gctgtttaaa aaacttttga agaaaaaaac gggataaagc ccctctttat
      901 ccccagttag tctttgtgat tatccttttc ttgggattgc ttatttaaat agtttacgtt
      961 tttaatctgg tgggggaaaa atcagaccac ggcagttccg tcggacccca tgttaaggca
     1021 ttttggtgca taaaagccat ctcccgcaat ggctggctgg atttttgcca ccactacaaa
     1081 tatttataaa caagatagca ctcgaaccct agttatggat ttgataagaa agtatgtcct
     1141 gcaaaactcg ataaaattca gtggaaaagc aagcgcaggt gcagtcatcg gaaagattat
     1201 tgcagaaaag cctgagctta aggaaaagat aaaggagatt gcagcacagg taaacaagga
     1261 gataaaggaa gtggcaaaga tccctttaaa ggagcaggaa gaggaattaa agcgggtggc
     1321 tccagaactc cttgaggaaa agaagcaaga aagcaaaaga gtcctgccgg agctaaaaga
     1381 cccaaaaaac cttgtgatgc gctttgagcc ttcgccatct ggccccctac atattggcca
     1441 tgcctatgtt ctaagcctaa attctgaata ctgccgcaaa tataacggta aactgatact
     1501 caggataagc gatacaaacc cgggcaatat ctgtgaccct gcatacgaaa tgatcccaaa
     1561 ggacgcccag tgggtcacaa aaagcaatgt aaaagaggtc catatccaat cagacaggct
     1621 tgctacctac tacaactata tggagcagct cataaacctt ggcaaagcat atgtgtgcac
     1681 ttgcgagcct gaaggataca aaaaactctt gcatgaaagt atagcatgcc cctgcaggga
     1741 acttccagtt gaagtgcaga tgaaaagatg gaagaggatg tttgatgaat acaagcaggg
     1801 agaggctgtg gtaaggataa agacagacct aaaccacaaa aatcctgcta tgagagactt
     1861 tcctgttttt aggataagtg agcttgagca tgcgaggcag gataagagat atagggtctg
     1921 gccgctcatg aacatggctg tctcggtcga tgatatagag atgaaagtca ctcatgcaat
     1981 aagggcaaag gatcattatg ataacgctga aaggcaaaaa tacatctatt cctatctaaa
     2041 caagaaattc cctcagacat tgtttgtcgg aaggataaac tttgaaggca tgccggtgag
     2101 ctgctcaaag acaaggcctt tgatagaaga cggcacatac atcggatggg acgacataaa
     2161 gctgcctttc cttgccgctt tgaagagaag aggatatcaa ccaggcgctt tcatcaagta
     2221 tgcgatagat gtaggcttaa gcctaaacga caagactgtc acaagagatg aattttttaa
     2281 gaccattaac gcgttcaaca aagatataat cgaccctgtc tcaaacaggt atttctttat
     2341 ctggaatcca gtagagataa ctatcgaaaa agcgccatct catgaggttg agcttgatct
     2401 tcatcctgat cataagaaag gcggaagaaa gttcagcaca agatcttctt tttacattac
     2461 aggcgaagac tttaaggcaa taaagggcgg aaaactgtac cgtcttatgg actgcttaaa
     2521 ttttcaaaag aaaggcagca agtttatatt tgactctctt gactatcagc agttcaagga
     2581 aaaaggagat aggataatcc attggctgcc aaaagaaaag ctgccagaag tagaggtctt
     2641 catgcctgat ggtgttatcc ataaaggcat aggagaaaaa gccctcactg atatgaaagt
     2701 tggcgatatc tgccagcttg aacgcctggg tttttgccgg cttgataaaa aagagaagaa
     2761 actaagtttt tggtatgcgc ataaatgagg tgatatcatg ggaaagtacc ttgttgtaag
     2821 gtccaacatc aaagattacg ccaagtataa cgaaaggccg ctaaacgtgt cagaagactt
     2881 ctatgaaaag ctcaatttaa aggttgcaga gataataaaa gaatcgtgta gaagagcaaa
     2941 ggaaaatagc agaaatacag tgatgggcaa agatatctaa gaaagccttg cttttatcgc
     3001 acctatcctc atctcaagct gtgtgcttga aatgcctttt ctgcacagct ccatatagct
     3061 ttgctcaagc ttatttagct ttgctgcaag cactgccttg ttctctttat gcgagtgtct
     3121 tatcttatgc tcaagctctt ctatcttccg ctctctttgg gtcttaacct ttacccatcc
     3181 atcaagcttt gtctgtaggt tatctacaat gctgtatatc tctttaatct tatccttgtt
     3241 cgtattcacg ccgtttacaa gctctctggt aagtacgctc tctttttgct ctattcccag
     3301 aagctgtacc tctttttcaa aaaggatatt tattttcttg ttgagttcga caagctctgc
     3361 ctcgatatct cccttcctgc tcgcaaattt cctgagcttc tgcatctgca gttgaagctc
     3421 ctttcttgct ttctccctgt cgctctgctt ttttgcgatg acgcaggcat gcttgatgaa
     3481 gcgctttagc cttccaggat ctatagaaag agtatcactc atttaaatat acccagcagc
     3541 ccttttttcc ttggctcttc ttcaaatggg ggcagcggtg gcagttcccc aaaatcaggc
     3601 gctggtgatc gcaactgccc cattccaggt ggcattggag gaggcatcat cccttgcata
     3661 ggaggcggcg ccataggacc cggacccatc attggcggca caggcggagg agcaaatcct
     3721 ggccctgact gccttctttg tgccttggct tttgaaaaga atgctggctc tctctcggtg
     3781 tcttcatcat cttcttcgtt catcgactgc ctgggagcca tccttttgtc tccttttatc
     3841 tgtttgacca tgtctgcgac agcgacaagc ccttctgcga ttgttctgtt ctgctgctca
     3901 aggtttgaaa ccttttctat aaggggccct atcttctgtg acagctctcc ttcagttttc
     3961 tcctcaagct tcatctcttc tgctgctgat gaaaagagct ttaacagctc atttactgat
     4021 tttgtaagtg cagccataga caaaagaagt tcctttgaag aagtatctcc tgcccttagt
     4081 tgcagctcag agatctgttt ctttagttcg actatctctt tataaggcat aatttcatat
     4141 gagccttcat cagtcataaa catcatcctc ttttagtgtg taatttaagc caacttcact
     4201 ctcaccaaaa gactatataa actttgctat ttcaaaaaaa taaagaaaaa agaaaggatt
     4261 gtttacatat ccattccggg cggcatccct cctggaggca ttgctgggcc tttgcttgag
     4321 ccgcttcctg cgataacatc atctatccta agtatcatca cagcaacctc agcagcagag
     4381 cttatcgcct gggtctttat ctttaatggt tcgattactc ctttcttcca tgagtcgacg
     4441 gctttgccct tgaatacatc tatccctgac catttctctc ctttgtcgtg ggctgcctta
     4501 agctcagtaa ggatatcgat aggatcaatt cctgcattct cagcaagtgt ccttgggatt
     4561 atctccattg catctgcaaa tttcatcaca gcaagctgtt ctcttccgct aaggctgtca
     4621 gcaaactttc taagctgctt tgcaacctct acctcgggag cgcctgctcc tcctactacc
     4681 ttcccatcct tgagcgcagc ggatatatcc ccgattgaat cttccatagc cctttttact
     4741 tcatcaacaa catgctctgt accgcccctt atgaggatcg ttactgcctt gggattcttg
     4801 cagtctttta cataggtcat ctgctcatcg ccgactttga cctcttcgac tgttccagca
     4861 aggccaaggt cttctttact taggtcgtca aggtttgtca ctacatttcc gcctgttgcc
     4921 ttagcaagtg caaccatgtc gctctgcttt gctctcctta ctgcatatat gcctttcttt
     4981 gcaaggaaat gctgtgcgat atcatctatg cctttctggc atattagcac attagctccg
     5041 gatgccgcaa tcttttcgac cttgtttctt agcatagtct cttcctggtc gatgaatgcc
     5101 tgcatctgtg atggatcggt tatctgtatc tttgcgtcga tctcagtgtt cttgatttcg
     5161 accgccgaat cgatcaatgc tatccttgcg cttttgacta ccctgggcat tccgccgtgc
     5221 actttttcct tatccaggat tattcccctg acaagctcgc tgtcttcgac agatgcacct
     5281 actttctttt caagcttgat gttttccttg tcgacagata gtctatcgtt ctgctgctct
     5341 gcaacagacc tgacagcttc aaccgcgata tccgcaagtt tttcttttga gtgctcggct
     5401 ccttttccgg tcattgcagt cattgcgatc tttcttaata tatctgtatc tttttcagaa
     5461 acatcttcag ctatcttatt taggatttcc tgtgccttgc cctctgcaag cctataaccc
     5521 cttgcaatta ttgtcgggtg gacattctgg tccagcaagt cctcagcatt cttgagaagc
     5581 tctcctgcaa tcacgacagc agttgttgtt ccatcgccga cctcatcttc ctgggtcttt
     5641 gctacttcca caatcatctt tgccgaagga tgctctatct gcatctcctc aagtattgtc
     5701 actccgtcgt ttgttacagt cacatctccc atggaatcga ctatcatctt gtccatcccc
     5761 tttgggccaa gcgttgtcct gacagtctct gcgacaagct ttgctgccat tatgtttgtt
     5821 ctctgcgcgt tccttccggt agtcctctgt gtgccttccg gcaggatgaa tattggttgt
     5881 atttctttac tcatatattc cacctccaaa ttagggagtt aagtacataa gcagtaatat
     5941 ttaaaggtat ctttttcttt tttcttcttg ttttcttgag ctgttaagtt agcaaaacct
     6001 tcttgtttgc ttacaattta catttaaact ttcaaccctc cctgcatcca gaggtaactt
     6061 gatcctttaa gcaaagtaat aaaaataagt ttattgattc caacttgatg aataaacttt
     6121 acattagcca agcaatacct caaatcgcct atttgcgccc caaaaatgca agggtccttg
     6181 ccacgcatat gctcaccaga tggcagcatg aaccagatga tcctgcccct gatctcaccc
     6241 tgataatccg ggaggactcg accctgttct atttcaggca gagctttgca gaatcccagg
     6301 catatatttt tagccaccag gtaccttatg acgagttttt gggcgacctg tcggatcttt
     6361 tccgcaccca gggggaggaa acagatataa ttcctgcaac ctatagggta aggcaaagca
     6421 tagaggaatt ggtaacacca tacgaatcag cccatattga agagataagc cctcttctgg
     6481 ttaccccgcc gccggaaata gcttctccca cattaaagca gataatcagg ctaagatatt
     6541 accctactca aactctttta gaaacttgat aagctcttca gctttttcaa actccataaa
     6601 ctcctttttg gtcatagcag gcacattttt tggctttttc ttgaggtaga tggcaagcct
     6661 gtcagcatcc acaagcttgg acaatgagtc aagtccttgc tgttgcccat ccccgacctt
     6721 tgtaagtatg agctctttct cttttttagc gatgatatca aatggcactt tttcagcttc
     6781 agttgcacta aaccccatat cagagaactt ccttgagata tctcctttac cgacaggcat
     6841 tatattctta tcatgcccaa agatatctat cttctcaaat agtgaatggc caagaagctg
     6901 atacagcctc aaggctttgt ttatgcttat cctgctttct cctgcctcat atttctgtac
     6961 catgctcttg ctgaccccta ccttccttgc caggtcgttg agggatatcc cttcctcttc
     7021 tcttaccttc ctcagctttg tgccgataag atgtgcattt acgcctgaat ggtcgcgtat
     7081 agcaaaaggt agcttgctgt cgatagagtt gcaaagagtt ctaaaattta gtgtaaatat
     7141 cccaaatctc agatacacaa cattatcttc cagtttgtct cctgccttat cagcaatgat
     7201 aagtgggctg ccgccaacat atgcagcaac attatgcatc gatttggtaa attcctcgct
     7261 taccgcgttg gcatcaaata atattttgat aagaaggata gttgtatccc ttctggctac
     7321 gatatcaaaa gctgtcctgt tcagtgtctt tacggtaaat ccttttctca aaagaaagat
     7381 tccaatcttc tcgacaagtg attccttcat caggtgtaga ttatctaaat cccttattaa
     7441 aagttacgtc tttcgacagc aggctcgata aaatgcagcc ggaacagcga cagcaagcat
     7501 gccagatata gacccgagga ttgctctgct tagccctgca agggtagctc cgacgtaagc
     7561 tacagctccg ccatataggc aagaaagata cctatatgcc ttcccagagc atagatccct
     7621 ccgatgattc ctccaagtat tcctgttcat caagggaaat atatatttct gtgattaata
     7681 aacttaacct ggcttggcct ccttcttagg ctttgcacca aaatattttg taatgggctc
     7741 aacaaatcca atgataattc tggcgccaag tattgtcgcc gcggctacag ctgctccggt
     7801 tacgatcaat gcaccatacg tggcaagcgg gctataggtt gcataaaagt tcatataaaa
     7861 ctccttgatt gcataggctg ccctgctagc atgccctaag atcgcttcaa gccccataga
     7921 tattcttggg cgatcaactc cctatttaat ccttgctctt tttaagtaat tgacactatg
     7981 ttgtccagca aaagaagcat tatattcatc agaacccttg aagcctaaaa cgaggtgaat
     8041 aaaaaaatga gagatgagat ggagatgggc aaagaaagta tgggaacaaa aagagggatg
     8101 tcagagtctc acggtccttt gactaagaaa ccttcgccga taggcatatc aaaatctttg
     8161 aaggggatag attttccaaa aaacaaggaa gaggtgaaac tctccagcct aacggctgaa
     8221 gcctctttct aataatctat tgcattgatg cccttggggc atagaagggg ataaacccct
     8281 tggattctga gtaacataaa tctttcccct tttgagatgg tgagccttcg gctc
//
LOCUS       DASRUK010000015         3414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2361731, whole genome shotgun sequence.
ACCESSION   DASRUK010000015 DASRUK010000000
VERSION     DASRUK010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2361731"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..334)
                     /locus_tag="VFF28_00685"
     tRNA            complement(join(225..260,295..334))
                     /locus_tag="VFF28_00685"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(296..298),aa:Lys,seq:ctt)
     gene            complement(373..717)
                     /gene="pth2"
                     /locus_tag="VFF28_00690"
     CDS             complement(373..717)
                     /gene="pth2"
                     /locus_tag="VFF28_00690"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010879586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-tRNA hydrolase Pth2"
                     /protein_id="HZX44177.1"
                     /translation="MGYKQVILVRQDLKLPKGKLAVQVAHASSTALIKSHKDDIKRWH
                     SEGMKKAALKVENLDELLKYKQLAEDAGLITALIEDAGKTVVEPGTITCLGIGPDKED
                     KIDKITGSLKLV"
     gene            838..2409
                     /locus_tag="VFF28_00695"
     CDS             838..2409
                     /locus_tag="VFF28_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44178.1"
                     /translation="MVNLEVSASGVNDVLDRSLEFIIDPDTDMILSEGPGALTVSNGD
                     GTIFSRRYFIQSVAAAATTSVLPAIGVKAEEFTWNPKVYDTTSLKGLKLGGLATSYTG
                     INGRVGDTVAVDFRQLLDNMWKKKMSISKGAEVNRFYETRVKEYLNPKFTPIRMNFVQ
                     YLTQVKEAIELVRSSLDWRKVGAIKGLDERRLRLVQNISYYLGEREVMAYSLTELCPS
                     SNGRANVKLIDFLLRNAGREYVELLPALGDKLLSYGPKQWTKWAVYEGKGGPRGASII
                     DQAMPQTIDLPGSVAELRGNQHFMASMEFIINNLAYTIKDLDEKSLATLESSWKNVQP
                     DIVQYIAVSHHAPRYGKEFMTDWLARKTINGKARFLDSCCDRFTIYAQKTAANHGALS
                     EGIVKSEPTSQRYQPKEAQKQAAAATTDTQSTAGRTDRSCAAGNAHNVQSNGPMFARI
                     GSGKYCQGWDTYRYIVQEGDTPLGIASRFNRSVWGYKPVGYANIKSPDDMFKGHYLKQ
                     LRSGQTINIVAKNLN"
     gene            2411..3373
                     /locus_tag="VFF28_00700"
     CDS             2411..3373
                     /locus_tag="VFF28_00700"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HZX44179.1"
                     /translation="MESLFDRRAFLKGICALAATELPSVQVYAASSAKNGIEALLHKS
                     LPQEVKNRMPLSADQLVEYVERSEQGKLTQIGRFLRTYRWHNLLSGLEKELEAPKGLL
                     AGLVLHESNGSPWSVGPTADAGLWMFIPETGRGYGLKIGPTSSLGLRKLLGDKKYDYT
                     AIAQKDERFDILKSAAAAKKQFVYLKGANATWNKALSAYNQGKAAPDPSSSDYVTKVR
                     EAQAFYNCQLRYLQDIAEGTQPKEIIKRLYERNRGLFWLENGIWHYRVIDGDIPGTIA
                     ALFNSKHGARYGNIKWTDIHYKGKKVIPEIIVPGQVVYWKVPKR"
BASE COUNT         1074 a          652 c          791 g          897 t
ORIGIN      
        1 aggttttatc ctgggcaaaa agaagaaata aggaaagaca acaaattgat tggagattta
       61 caaagcagat ggctgatgag aaactgtcta agcattatgt aatctaacta agttgtcaag
      121 ctactagggc ttcggttaaa ggcatatgtt aaactcaatc gagaaagagg gctaacgact
      181 gaatgggggc tttggcttgt tatataacgt agttttgaaa aaagcgggcc cacggagatt
      241 cgaactccgg acctattgct tgctataaga caagcaatgt aagcttgttt ctgttaagag
      301 gcaatcgctc taaaccaggc tgagctatgg gcccaatgac ataaagaaaa aatactttat
      361 ttataaatct attcaaacga gcttaagact gcctgttatc ttatctatct tatcctcttt
      421 atcaggcccg attccaaggc aggttattgt gcctggctct accactgtct ttcctgcgtc
      481 ttctatcaaa gcagttataa gaccagcatc ttctgccagc tgcttgtact ttaagagttc
      541 atcgaggttt tctaccttta aagctgcttt cttcatgccc tcactatgcc acctttttat
      601 atcatcctta tgcgacttta tcagggcggt agagcttgca tgcgctacct ggacagctag
      661 ctttccttta ggaagcttta ggtcctgcct taccaaaatt acctgtttat aacccataca
      721 attttaaagc cggcttggac tatataaaat tattcctagg cggatatagt caattaatca
      781 ctaaatagta gcaataatac gtaaagttta taaagagaag atacctttag gttttgtatg
      841 gtcaacttgg aagtatcggc gagtggagtg aatgatgtgc ttgataggtc actagagttt
      901 atcatagacc ctgatactga tatgattcta tctgaaggtc caggagcact cacagtcagc
      961 aacggtgatg gcacgatttt tagcagaaga tattttatcc agtcggttgc agcagccgca
     1021 actacatctg ttctacctgc gataggagtc aaagctgagg agtttacatg gaatcctaaa
     1081 gtgtatgaca caacctcttt aaaaggactg aagctggggg gactggccac ttcctataca
     1141 ggtataaatg ggagggtagg agatactgtt gcagtcgatt ttaggcagct tctggataat
     1201 atgtggaaga aaaagatgtc tatatccaag ggcgctgaag ttaataggtt ttatgaaaca
     1261 agggtaaaag aataccttaa tccaaaattt actcccataa ggatgaattt tgtccagtat
     1321 ctgactcaag tcaaggaggc tattgagctt gtaagaagca gtcttgattg gagaaaggtc
     1381 ggcgccatta aaggattaga tgaaagaaga cttagactag ttcaaaacat ctcatattat
     1441 ctgggagaaa gagaagtcat ggcttactcc cttactgaac tttgcccgtc ttcaaatgga
     1501 agggctaacg tcaagcttat tgattttttg ctaagaaacg caggcagaga gtatgttgaa
     1561 ttgcttccgg ctcttggcga taaattacta tcctacggtc caaagcaatg gactaaatgg
     1621 gctgtctatg aaggaaaagg agggcccaga ggagcatcga taatcgatca ggcgatgcct
     1681 caaaccatag atcttcctgg aagcgtcgca gagcttaggg gcaatcaaca ctttatggct
     1741 tctatggagt tcataatcaa taatcttgcg tatacaatta aagaccttga tgaaaaatcc
     1801 cttgcaacct tagaaagctc ctggaagaat gtacagcctg acattgttca gtatattgca
     1861 gtttcacatc atgcgcccag atatggtaaa gaatttatga ctgattggct tgcaagaaaa
     1921 acaatcaatg gaaaagcaag gttccttgac tcgtgttgtg acagattcac catttatgct
     1981 cagaagactg cagcaaatca cggagctttg tcagaaggga tagttaaatc agagccgact
     2041 agtcaaaggt atcaaccaaa agaggcacaa aaacaagcag ctgcagctac gaccgatact
     2101 caaagcacag caggaagaac agacaggtca tgcgctgcgg ggaatgccca taatgttcaa
     2161 tctaatggtc caatgtttgc caggataggc tcaggcaagt attgccaagg atgggataca
     2221 tatagatata ttgtccaaga gggtgacact cccttaggca ttgcatcaag gtttaacaga
     2281 agcgtatggg gatacaaacc tgttggttat gcaaacatca aatcacctga tgatatgttc
     2341 aaagggcatt atttaaagca gctaaggtct ggacaaacga tcaatatagt tgcaaaaaat
     2401 ctgaattaag atggaatcac tttttgatag aagggccttt ctaaaaggga tatgtgctct
     2461 agcagcaact gaattgccaa gtgttcaggt ctatgcagcc tccagcgcaa aaaatggcat
     2521 tgaagcgctt ttgcataaaa gtttgcccca ggaagttaaa aaccggatgc cgttaagtgc
     2581 tgaccagctc gtcgaatatg ttgaaagatc agagcaaggc aaattaaccc aaataggccg
     2641 gtttcttcgg acgtataggt ggcataatct tctatctggg ctggaaaagg agcttgaagc
     2701 tcccaaggga ttgcttgcag gccttgtcct gcacgaaagc aatggaagcc catggagcgt
     2761 gggccctaca gcagatgcag gcctttggat gtttatccct gaaacaggac gaggatatgg
     2821 cctgaagata ggcccaacaa gcagcctggg cctaaggaag ttattgggcg ataaaaaata
     2881 tgattatact gcgatagccc agaaagatga gagatttgac atccttaaga gtgcagctgc
     2941 agcaaagaag cagtttgtat atctcaaggg tgcaaatgca acatggaata aggcactctc
     3001 tgcctacaat caggggaagg cagcgcctga tccatcctca agtgattatg taacaaaagt
     3061 aagagaagcc caagcgtttt acaactgcca gcttagatat ctgcaagata ttgctgaggg
     3121 tactcaacct aaagaaataa tcaaaagact ctatgaaaga aatcggggtt tgttctggct
     3181 ggagaatggc atttggcatt acagggtcat cgacggtgac attcctggga ctatagcagc
     3241 actgtttaat agcaagcatg gggctaggta tggcaacata aagtggacag acatccatta
     3301 taaaggtaag aaggttatac ctgagataat tgtcccaggc caggttgttt attggaaggt
     3361 acctaagaga tagatatttt accaccaata ggtagctaat caatcgaaac attt
//
LOCUS       DASRUK010000016         2658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2362229, whole genome shotgun sequence.
ACCESSION   DASRUK010000016 DASRUK010000000
VERSION     DASRUK010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2362229"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..1358
                     /locus_tag="VFF28_00705"
     CDS             222..1358
                     /locus_tag="VFF28_00705"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HZX44180.1"
                     /translation="MVKVAVVKCSDYDTGKVISAVKESLNLINYKVPKGKKILLKPNV
                     LGQNKPETAIDTHPAVIDAICTIFSGNELWIGDSGGITAYGGTRQAFEVSGIADIARK
                     HKARLISFEASKEKHVIVDQNNKVLKKFTIAKEPFMVDLIINVPKLKTHMLTKYTGAV
                     KNMFGCIPGGGKSQKHAIAPTEESFSNCLLDIYTHVKPHLNIMDGIIGIEGNGPGSGG
                     TPKKAGLILASEDAVALDIVASKTIGYNPLDIKTIRYAIERGIFPGLSAVEKRGDEAF
                     IEFKKPATAGRRKLPKFLMKFAFSLASIKPYIVKKRCRKCGVCRTACPVKAISLKPYP
                     IFDRKKCILCYCCHENCPYKAIYLHKSTVIKAYQKVKGWQSNKI"
     gene            1396..>2658
                     /gene="pgk"
                     /locus_tag="VFF28_00710"
     CDS             1396..>2658
                     /gene="pgk"
                     /locus_tag="VFF28_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HZX44181.1"
                     /translation="MGRYPTVKDIDIRKKLVIVRCGLDVPLDSKKDLLDPARVTDDTR
                     IKDIIPTLRYLIENDAKVILAAGWCGRPKGEDADYSMAPVAKRLEKILKENGLLRADV
                     LIAPNSYDGRPRSVYKNKEEVKRIVQNLSKSQVVVLENVRYDSEANANDKEFASFFAS
                     LGQVYINENEVQNHRPEATIVEVPKIIAKKGGRVAYGFQYANVLENIGGLQERLKNPN
                     RAPFVFGLCGKKIESDPGITSKITVALDLIDKMKKGDVIITGGAVVYTFLLAEHYKGK
                     IEANLSKVKDMVARYNKDIVEKTKSTKDKEAAKKLVDDLQKEKSSGLKELLGITDSEI
                     KSLVGSSYIRWGQEGEQIVFAYDLMAKASAKGIEVITAIDHTITNSPPDKAGKLPESA
                     EIKIHPSRTGIPLGYLGIAEGPKTLDKIS"
BASE COUNT          902 a          500 c          594 g          662 t
ORIGIN      
        1 ctcgcatttc tgattttggc agaagacatc taatttcctt cttttctttg gcatgatatc
       61 acctttatta ttgacgaaca ataatatata tttttagaat tagcttatat ttatatcttt
      121 cgtttcagta gtcatactct aatttaggaa accccctctc caagataaga gcattgttgg
      181 atttaacaat ccttaaataa caatttaatt tccctattct tatggtcaaa gtagcagtcg
      241 ttaaatgctc tgattatgat acaggtaaag taatcagcgc agtaaaagag tccttaaacc
      301 tgataaacta taaagtgccg aaaggaaaaa agatccttct aaagccaaat gttcttggcc
      361 agaacaagcc agagactgcg attgacaccc accctgctgt gattgacgcc atctgcacta
      421 tcttctctgg aaacgagctc tggatagggg attcaggagg aataactgcc tacggcggca
      481 caaggcaggc ttttgaggtc tcagggatcg cagatattgc aagaaagcac aaggcaagat
      541 tgatatcatt tgaagcttca aaggaaaagc atgtgatcgt tgaccaaaac aacaaagtcc
      601 taaaaaagtt cacaattgca aaagagccgt ttatggttga tttgataata aatgtcccaa
      661 agctaaagac ccatatgctc accaaatata caggcgctgt caaaaacatg tttggctgca
      721 tccctggcgg aggaaagtca caaaagcatg cgattgcacc aactgaggag tctttctcaa
      781 actgtctatt agacatttac acccatgtca agcctcactt gaatataatg gatgggataa
      841 taggcattga aggaaacggt cctggctcag ggggcactcc caaaaaagca ggcctaatcc
      901 tggcatcaga ggatgcagtc gccttagata ttgttgcttc aaaaaccata ggttacaatc
      961 cgctggatat taaaacaata aggtatgcga tagaaagggg gatctttcca ggtttgagcg
     1021 cagtagaaaa gagaggtgat gaagctttca tcgaattcaa aaagccagcc actgcaggaa
     1081 gaagaaagct cccaaagttc ttgatgaaat ttgccttttc gcttgcttcg ataaagccat
     1141 atatagtaaa gaaaaggtgc aggaaatgtg gcgtctgcag gacagcttgc cctgtcaagg
     1201 cgatatcatt aaagccctat ccgatttttg atagaaaaaa gtgcatactc tgctactgct
     1261 gccatgaaaa ctgcccttac aaagctatat atctgcataa aagcacagtc ataaaagcct
     1321 atcagaaagt aaagggatgg caaagcaaca aaatttaaat atgcaatagc ttaataactt
     1381 caaggggtga gttttatggg cagatatccg acagttaaag atatagatat tagaaaaaaa
     1441 ttggttattg tacgctgtgg cctggatgtc cctttggatt ccaaaaagga tttgctggac
     1501 ccggcaagag ttactgatga cactaggata aaggatatta tccctactct ccgctatctt
     1561 atagaaaacg atgcaaaggt gattcttgct gcaggttggt gtggcaggcc aaaaggagaa
     1621 gatgctgatt acagcatggc tcctgtggca aaaaggcttg aaaagattct aaaggaaaac
     1681 ggattgctaa gagcagatgt tcttatcgct cccaattcat atgatggtag gccaagaagt
     1741 gtctataaaa acaaggaaga ggtaaaacgt attgtccaaa atctgagcaa aagccaagtg
     1801 gttgtccttg aaaatgtcag atatgactca gaggcaaatg caaacgacaa ggaatttgca
     1861 tcattctttg cttcccttgg acaagtgtac ataaatgaaa acgaagtcca gaaccatcga
     1921 ccggaagcaa cgatagtaga agtgccaaag attatcgcaa aaaaaggtgg ccgggttgct
     1981 tatggctttc agtatgcaaa tgtgcttgaa aacataggtg ggttgcagga aaggctaaag
     2041 aacccgaata gggctccttt tgtattcggt ctttgcggta aaaagataga gtcagaccca
     2101 ggcatcacat caaagataac tgtcgctttg gacctgatag ataagatgaa aaaaggggat
     2161 gttatcataa caggaggcgc agtagtctat acctttcttc ttgcagagca ctataaagga
     2221 aaaattgaag ccaatctctc caaggtaaaa gatatggttg ccagatacaa caaagatatt
     2281 gttgaaaaga caaaatcaac aaaagacaag gaagcggcaa aaaagcttgt tgacgatctg
     2341 caaaaggaaa aaagtagtgg cttaaaggag ctgcttggga ttacagactc agagataaaa
     2401 agccttgttg gaagctcata tatccgatgg ggccaggaag gcgagcagat tgtttttgct
     2461 tatgacttga tggcaaaagc cagtgcaaag ggcatagagg ttatcactgc cattgaccac
     2521 accatcacaa atagtcctcc tgacaaggca ggaaagctgc cggagagcgc agagataaag
     2581 atacatccat cgagaacagg tatcccctta ggttatttag gcatagctga agggccaaag
     2641 actctggata agatatct
//
LOCUS       DASRUK010000017         2151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1529256, whole genome shotgun sequence.
ACCESSION   DASRUK010000017 DASRUK010000000
VERSION     DASRUK010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1529256"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..695)
                     /locus_tag="VFF28_00715"
     CDS             complement(240..695)
                     /locus_tag="VFF28_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44182.1"
                     /translation="MTKLGFKTRSKPISHDKLDIFEMLENDDFRFDLFVNTVVGGFSL
                     SKEMAKRSQNILGLKYLTAFVCSKEDIVLFKSLSPDRQNDIEDSLDLIKRGIEWNIIY
                     QELLIQLSICNPKRGKELVWYFIERIHDLERKGAVVPIKAKIEQVYDKI"
     gene            complement(676..801)
                     /locus_tag="VFF28_00720"
     CDS             complement(676..801)
                     /locus_tag="VFF28_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44183.1"
                     /translation="MSEIALGARSDVLTQCKPAISEHAQEHAPSFSSAVLDKIGL"
     gene            complement(783..992)
                     /locus_tag="VFF28_00725"
     CDS             complement(783..992)
                     /locus_tag="VFF28_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44184.1"
                     /translation="MIYKFEALNELFKQIDGQLSGKVSLFILGGAALLHYGVGKGYTK
                     DIDIVFDNYNQFVVFKHALTCQKSL"
     gene            complement(976..1869)
                     /locus_tag="VFF28_00730"
     CDS             complement(976..1869)
                     /locus_tag="VFF28_00730"
                     /inference="COORDINATES: protein motif:HMM:NF019884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HZX44185.1"
                     /translation="MRLSFTQLRIIEFISQETNVSVKDISSHIHISPSMAYKHVKELT
                     AAGFLDKIGAKRRQKYSISSNAFARILCSLFAEDSAIKKTLANEGIPLLARLAEERKT
                     LKELAKEAGLSLSSAYPYIKRFTDRQILRKINGSLEFNEELWPQLHQFIALYLNYYAL
                     SRFKVPANAKIHFESADEIVYSLKDETAFGEKTAFSAYGRYGIELMENEVFYRQDNLH
                     KRPDIQTIFADSLKAARGSPRRRLYCYLFYRKNIKSLGKVKHPDLDILKKIASGIMEK
                     SEGFPTRSEILEKGKDYDLQV"
BASE COUNT          593 a          446 c          423 g          689 t
ORIGIN      
        1 aaccataggt gatccggtac agttcaaaaa aattaaggat cctattatcg gaatcattga
       61 gcaaaacagg aacggttcat tcaggcatcc ctagaggcaa gacactgggc aataaattat
      121 tttagttcta aggagaaatc aggtaatcgg tgctaaatta gctgctcaaa gcgattgaag
      181 aggcttaagt aaacgcgcct ctagctatca caaggtcgat tatgatacca ttaagggacc
      241 tatattttat catagacttg ttcaattttt gccttgatgg gtacgaccgc ccccttgcgt
      301 tcgagatcgt gaattcgctc gataaagtac cagacaagtt ctttccccct ttttggattg
      361 cagatgctaa gctggatcag cagttcctga tagattatgt tccattcgat gcctcttttg
      421 atcaggtcca gggaatcttc gatatcattt tgcctgtcag gggataatga cttgaagagc
      481 acaatatcct ctttgctgca gacaaaagcg gttagatatt ttaaacccaa gatgttctgc
      541 gaacgttttg ccatctcttt gcttagggag aatcccccga ccacggtatt gacaaataga
      601 tcaaacctga aatcatcatt ctcaagcatt tcgaatatat ccagtttgtc atggcttatc
      661 ggcttgcttc tggtcttaaa gcccaatttt gtcaagaacc gccgagctaa agctcggggc
      721 gtgttcttga gcatgctcag aaatcgctgg cttgcactgg gttaaaacat cagagcgtgc
      781 gcctaaagcg atttctgaca tgtcaaggca tgcttaaaca ccacaaattg gttatagttg
      841 tcaaagacaa tatcgatatc tttcgtatat cccttgccaa caccgtagtg aagcaatgca
      901 gctccaccta atataaacaa ggatactttg ccgcttagct ggccatcgat ctgtttaaac
      961 agctcattga gagcctcaaa cttgtagatc atagtccttt cccttctcaa gtatctcgct
     1021 tcttgttgga aatccttcgc ttttctccat aattcccgat gctatctttt tcaggatatc
     1081 cagatcagga tgctttacct ttccaagtga tttgatattt ttcctgtaaa aaagatagca
     1141 gtaaagcctt cttcttgggc tgcccctggc agctttcagc gaatctgcga atattgtctg
     1201 gatatccggt ctcttatgca gattatcttg cctgtagaat acttcatttt ccataagttc
     1261 tatgccatat ctgccgtagg ctgagaaggc ggtcttttct ccaaatgcag tctcatcctt
     1321 taaagagtag acgatttcat ctgcgctttc aaaatggatt tttgcatttg caggcacctt
     1381 aaacctggat agtgcataat agtttaggta taatgctatg aattggtgca gttgaggcca
     1441 tagttcctca ttaaactcaa ggctgccatt tattttcctg agtatctgcc tgtcagtaaa
     1501 cctctttatg tacggatagg ctgaagacag gctcaggccg gcttcctttg ccagctcttt
     1561 taatgttttc ctctcctcgg caagtcttgc aaggaggggt atcccttcat ttgccagtgt
     1621 cttttttatt gcgctgtcct ctgcaaaaag gctgcaaagg attcttgcaa aagcatttga
     1681 tgagatgctg tatttttgcc tgcgctttgc tcctatcttg tcaagaaagc cagccgcagt
     1741 cagttctttg acatgcttat aggccattga agggctgatg tggatatgac tggagatatc
     1801 tttaaccgac acattagtct cctggctgat aaattctatt atccttagtt gagtaaaact
     1861 tagtctcatt tttatcaata aagttcattt ctttataaat atttctaatt ttttgataaa
     1921 atctcgattt tcagcaaggg attatcaaat cagatgatca ctgattcatt gaatagaaga
     1981 gccaaagaaa aaatgttttc tgatgaagat aggcttcagt taattatagt aagttaaata
     2041 gaaaagttct tatataagta ccccttctgt aattagcaag aggtgatata gatggcatta
     2101 agaaccaaat ttcatcattc gtcaagtatt tttaagagac tgtataacaa a
//
LOCUS       DASRUK010000018         8258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1192413, whole genome shotgun sequence.
ACCESSION   DASRUK010000018 DASRUK010000000
VERSION     DASRUK010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 8258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8258
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192413"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1619
                     /locus_tag="VFF28_00735"
     CDS             <1..1619
                     /locus_tag="VFF28_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HZX44186.1"
                     /translation="SGTRACSHGQWSICSSSGISCSYDCPDSCNIDPDGFSNTMDIGA
                     SYLSTCDSNGQCRNMQSCNYSHYCSDNDLADSWPPVQDAIKCNALCDKDNDCMDTDCS
                     FLDKCYDGTFREYANVKNGCQGNCACTKKDCSIYEEIIHDNDHDGYDTDCDDDCDDWN
                     QSIRPGAYESCNKIDDNCDGKIDEGCCYSNWVLDNSTCQIDDKKLMRYIDTNNCGTKE
                     VLPQENGTYLNCDYCNSTWVSKSSSCGPENYLNISYNYTNNCCALTGLVSDCNIPKDT
                     AAWCDYCTPYLKNGSWSSWVDVGCLPNDTMNQSRSRVQSDANNCGEIGDKTFVEYRAS
                     ESCDYCRPGWILDDTPCEQNNQKLISYHDLNYCNESQNLPVDNGTYSKCNYIYSFNIS
                     LTEGWNLISIPLNLENRTLPEPFASIRGQYAKLFAYQDGKWVELIDGSDIDETMGLWV
                     NIIHNTTLMVKGEEIQNFTYSLPIGRNLVGYPSLNKASIGKVFNLSNIMLISLYNNSK
                     WSTFTPKRPYSKNQLIEIHPGLGYWVELSE"
     gene            1687..2454
                     /locus_tag="VFF28_00740"
     CDS             1687..2454
                     /locus_tag="VFF28_00740"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZX44187.1"
                     /translation="MVVKQELLNKLKNDFRLNIYEAKIWTVLLSRGIATAGELADLSG
                     VPRSRCYDVLESLEKKGFIIMKIGKPIKYIAVQPEEIIERVKKDISEDTDLSLRMIEE
                     IRGTDVFKELQLLHKTGIEKVDGSELSNSIIGRENINQFLKRMVESAEKSIIISTDAE
                     GFSRKVNTLHKPLKKVASKGIKAKIIAPIDKKSAVKVEGVAELVDKDINGRFVMVDDQ
                     LLFMVNDSAVNKHYECAVWVKSAFFTSALREMVSSLY"
     gene            2464..3315
                     /locus_tag="VFF28_00745"
     CDS             2464..3315
                     /locus_tag="VFF28_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44188.1"
                     /translation="MDYSGYAATGRVLSGKTTVQIIDIPQIAMPLPLVCLDGQKLEDI
                     VSVRLDEKGGVPSAYFLNNNPDFKGPLRKGRYLLHGQVEGFPFTFQQFNERCPVGGIE
                     VIYYMGLVYDILHQAATARVYKVRASRASHILNEHRETPKDLWIVNKQFIPSLTTMAI
                     LVDIGGSYINGEYAPYTEVIHYWKTLIEGHAGKNLVPIEIQSKGVYTREPNAFCAEAM
                     DDEDPPQPTENLTPTITDRMQKEKEARQRREQALKKWARLHAETEMPPDNESVSVSSR
                     WKLKKRK"
     gene            3371..4015
                     /locus_tag="VFF28_00750"
     CDS             3371..4015
                     /locus_tag="VFF28_00750"
                     /inference="COORDINATES: protein motif:HMM:NF012452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HZX44189.1"
                     /translation="MVEDKDIRKTGTTTVALVCKDGVVLCADKRVTSGYLVAYKKYDK
                     IVQINDDIAVTVAGTVSDVQLLVKLLRAELKLKDLRSNRPVSVKEAVNLLSNMVYNNI
                     RKFSVIPGISHFIVGGKDDEGVHVYDVGADGSATEIDEYTSSGSGSVMAYGVLETLYK
                     KDMTVEEGVKLGAKAINAAVQRDIASGNGIDIVVITKDTLKKVFSKLIEFKIEA"
     gene            4045..5940
                     /locus_tag="VFF28_00755"
     CDS             4045..5940
                     /locus_tag="VFF28_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-CASP ribonuclease aCPSF1"
                     /protein_id="HZX44190.1"
                     /translation="MADITKEILKSLPEGSISDVAFEGANIVLYTKDKEFFLDNNGLI
                     KRIVDDIKKRVELRPDPAITMDMEKAEAEIKKIIPEEAGIDQVIFDPHRSRVIIEAAK
                     PGLAIGKQGAFLSEIRTKTLWVPLIRRKPAIRSKLIENIRAVLYKESDYRRKFLDKTG
                     HRIYDGWMREKKHEWVRVSYLGGGRQVGRSCLFLQTPESRVLLDCGVDVASSDQEAYP
                     LLEAPEFNIQELDAVIIAHSHLDHSGFVPYLYKYGFRGPIYCTEPTRDVMALLALDYV
                     KIMRGEGKEPIYSTDDIKEMVKHTITLEYDEVTDITPDIRITLYNAGHILGSAMIHLH
                     IGNGLHNLLYTSDMKYGRTRLLEPAVTDFPRLETLMIEGTYGGKDNLLPPRQEQEELI
                     RRKIDETIKRGGKVLIPVLGSGRAQEVMVLLESFIRNGQLDKVPIVVDGMVWDITAIH
                     TAYPEFLNSVVRKLIFHKDQNPFLSEVFREIGSQKERMQVIEEMGPCVILATSGMLMG
                     GPSVEYLRHLADNPNNCLLFVAYQGEGSLGRKIQRGNKEIMFGTAMRPEICPVRMEVV
                     TIEGYSGHSGRKELMTFMLKCNPKPKKVIVNHGENSRCLDLASSIHKQNRVETVCPRN
                     LEVIRIK"
     gene            6193..6966
                     /locus_tag="VFF28_00760"
     CDS             6193..6966
                     /locus_tag="VFF28_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44191.1"
                     /translation="MQDTYANLAQMIGMIKTSRNLGYHNEAPELSVKLFSGCRSIEEL
                     AAKAKLQGDEQTKLRHGCALLYKTAELMMGSPDARVYLDCLISNEPGLSSLHAHAAMV
                     GIDYAAQCLENKALLTPKELDKLAFDKCAKRYDNVAFYNMPSVAIVHDRGTVVDIHEI
                     KDETRMKEINAPKIGYLEGGFLIIPHSEPFTGTAQEAVDHLHKRGQPNFILTTEVMLA
                     HYIANRYTLMRLWEAFKTSKIPLDDLSKLTKAGVIFDNG"
     gene            7053..7583
                     /locus_tag="VFF28_00765"
     CDS             7053..7583
                     /locus_tag="VFF28_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44192.1"
                     /translation="MENEELEVRKVACHTCGKLFEVVVPKDYMTGDIFFCEGCSGIKI
                     FEFKVDGLPPIKDGANSMWNKPEMAERIRALRVGAKAALNGYVLKNDITLAVEIECMG
                     DCGDLDNFVAGICDCLQIANTKPQFIHPSLRNDPSIDPATVCFIEDDSKIMRIESSIN
                     KKASKNGYLIELYDEK"
     gene            complement(7729..7911)
                     /locus_tag="VFF28_00770"
     CDS             complement(7729..7911)
                     /locus_tag="VFF28_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44193.1"
                     /translation="MTNPLLQALEELRKEEKLKEVKKKRAPFGLRKEKRLQYYSGWHK
                     DDKPVTETRIEQQKKL"
     gene            complement(7904..8029)
                     /locus_tag="VFF28_00775"
     CDS             complement(7904..8029)
                     /locus_tag="VFF28_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44194.1"
                     /translation="MPEETGIKVCPMCNEVNKISLGQKELRCMKCGYKILEEKND"
BASE COUNT         2665 a         1535 c         1920 g         2138 t
ORIGIN      
        1 gcagcgggac aagggcatgc agccatgggc aatggagcat atgctcatcg agcggcatct
       61 catgcagcta tgattgccct gattcctgca atatagatcc cgacggattt tccaacacta
      121 tggatatagg agcatcctac ttatctacct gcgatagtaa tgggcaatgc agaaacatgc
      181 aatcatgcaa ttattcgcat tactgttcag acaacgactt agcagacagc tggccgccag
      241 tacaggatgc tatcaaatgc aatgcattat gtgacaagga caacgattgt atggatacag
      301 actgcagctt ccttgataaa tgctatgatg gaacgttcag agaatatgca aatgtcaaaa
      361 atggctgcca aggcaactgc gcctgcacaa aaaaagattg cagcatatat gaagagatta
      421 tacatgacaa tgaccatgat ggttatgaca cagattgcga tgatgattgt gatgattgga
      481 atcagtcgat aaggcctggg gcctatgaat cgtgcaataa aatcgatgat aattgcgatg
      541 gcaaaattga tgagggttgc tgctattcta attgggtttt ggataacagc acttgtcaaa
      601 tagatgacaa aaagctgatg agatatatag acacaaataa ctgcggtaca aaagaggtgt
      661 tgcctcaaga aaatggaaca tacttgaact gcgattattg caattcaaca tgggttagta
      721 aaagttcaag ctgcgggcct gaaaattatt tgaatatctc ttataattat accaacaatt
      781 gctgtgcctt aactggcctg gtatcagact gcaatatccc taaggataca gctgcttggt
      841 gcgactattg cacgccctat cttaagaatg gatcttggag cagctgggtg gatgtcggat
      901 gcctgccaaa tgacacgatg aaccaaagca ggagcagggt gcagtctgat gcaaacaact
      961 gcggcgagat aggcgataag acctttgttg agtatagggc ttctgagtca tgcgactact
     1021 gccgcccagg ctggatattg gatgatactc catgcgaaca aaataatcaa aagttgataa
     1081 gttatcatga tttaaattac tgcaatgaat cacaaaattt gcccgtagat aacggcacat
     1141 atagtaaatg caattacata tacagtttta acatctcact aaccgagggg tggaacctta
     1201 tctctattcc tttaaatctt gaaaacagaa ccctacccga accgtttgct agcataagag
     1261 ggcaatatgc aaaattgttc gcgtatcaag acggaaaatg ggttgaatta atagatggct
     1321 ccgacattga tgaaacaatg gggctttggg tgaatattat acacaataca acattaatgg
     1381 taaaagggga ggaaattcaa aatttcactt atagtcttcc tattggccgg aaccttgtag
     1441 gctatccaag cctaaacaag gctagcatag gcaaagtatt caatctttca aatatcatgc
     1501 taataagctt gtataataac tcaaaatggt cgaccttcac cccaaaacgg ccttactcta
     1561 aaaatcagct gattgagatc caccctggtt tgggttactg ggttgagcta agcgaatgaa
     1621 tagtatcact taagagtaaa aacaaacgaa agatttaaat agcactctat catcctaaag
     1681 gatagaatgg tagtcaaaca agagctcttg aacaagctga aaaacgattt tcgcctcaac
     1741 atctatgaag caaagatttg gactgttctt ctgtcaaggg gcatcgcaac agcaggagag
     1801 cttgctgact tgtcaggcgt tccaagatca agatgctatg acgttctaga aagccttgaa
     1861 aaaaaaggtt ttatcataat gaagatagga aagcctatca aatatatcgc tgtgcagcct
     1921 gaagagataa tcgaaagggt aaaaaaagac ataagcgaag atacagatct aagcctcagg
     1981 atgatagagg agataagggg cactgatgtt tttaaagaac tgcagctgct tcataagacg
     2041 ggcatagaga aggttgacgg cagcgagctt tcaaattcca taatcggaag agagaatatc
     2101 aaccagttcc taaaaaggat ggtagaaagc gcagagaaat cgataataat ctccaccgac
     2161 gcagaaggtt tctcaagaaa agtcaatact ctgcataagc cattaaaaaa ggtagctagt
     2221 aaagggataa aagcaaagat aatagccccg atcgacaaaa agtcagcggt aaaggtagaa
     2281 ggcgttgctg agcttgttga caaggatata aacggaaggt ttgtgatggt tgatgatcag
     2341 ctcttgttca tggtaaacga cagtgctgtt aacaagcact atgaatgcgc tgtctgggta
     2401 aagtctgcgt tctttacaag cgctcttagg gaaatggttt ctagtttgta ttaggtgagt
     2461 ttaatggact atagtggata tgcagcgaca ggaagggtgc taagcggtaa aacaacggta
     2521 cagataattg atatacccca gatagcaatg ccacttccgc ttgtttgcct ggatggccag
     2581 aaacttgaag atatagtctc cgtaaggctt gatgaaaagg gcggtgtgcc atcagcctat
     2641 tttttgaaca ataatcctga ttttaaagga ccgttacgca agggacgcta cttacttcac
     2701 gggcaggttg aagggttccc gtttaccttt cagcagttca atgagagatg tccggtgggt
     2761 ggcatagaag taatttatta tatgggcctt gtgtatgata ttctccatca ggccgcaaca
     2821 gctcgtgtct ataaagtcag agcaagtaga gctagccata tcttaaacga gcacagggaa
     2881 acaccgaagg atctttggat tgttaacaaa caattcatac ctagccttac taccatggca
     2941 atactcgtcg atataggggg tagttatata aacggagaat atgcaccata cacagaagtg
     3001 atccactact ggaaaacgct gatagagggg catgcaggga aaaatctagt gcctatcgag
     3061 atccagagca agggagtcta taccagagag ccaaatgctt tttgtgccga ggcaatggac
     3121 gacgaggatc ctccgcaacc aacagaaaat ctcaccccaa caataacaga caggatgcaa
     3181 aaagaaaaag aagcgaggca aagaagagaa caggcgctta agaagtgggc aaggcttcat
     3241 gcagaaaccg agatgccacc ggataatgaa agtgtatcgg tttcatcccg atggaaactg
     3301 aaaaaaagaa aataaaatcg caacatttat atattacctt atcccaacac ccaattaggg
     3361 aattctgaga atggtagaag ataaagacat aaggaaaacc ggaactacaa ccgtagcttt
     3421 ggtatgcaaa gacggcgttg tgctttgtgc agataaaagg gttacatctg gatacttggt
     3481 agcatataaa aaatatgata agatagtcca gatcaatgat gatatcgctg ttacggtcgc
     3541 agggactgtt tctgatgttc agctcctggt caagcttttg agggcagaac tcaagctaaa
     3601 ggatcttaga agcaataggc ctgtcagcgt taaggaagct gtaaaccttc tttcaaacat
     3661 ggtctataat aatataagaa aatttagcgt catcccaggt atttctcatt ttattgttgg
     3721 tggaaaagat gacgagggag tacatgttta cgatgtaggg gctgatggaa gcgccacaga
     3781 gatagacgaa tacacgagct caggttcagg ctcggtcatg gcttatggtg ttttagagac
     3841 attatacaag aaagatatga cagtcgaaga aggtgtcaag cttggagcaa aagccatcaa
     3901 cgcagcagtg caaagagata tcgcttcagg aaatgggata gatattgtag tgataacaaa
     3961 agacaccctt aaaaaagtgt ttagcaaact tatagagttt aagattgagg cataagaagc
     4021 aattcttaaa aattattgat attcatggca gacataacta aagaaatttt aaagagcttg
     4081 cctgagggaa gcatcagcga tgtagccttc gagggagcaa acatcgtttt gtacacaaaa
     4141 gacaaggaat tctttcttga taacaatggg ctaatcaaaa ggatagtcga cgacatcaag
     4201 aaaagggtag agctaaggcc agatccagcg ataactatgg acatggaaaa ggcagaagca
     4261 gagataaaaa agattatccc cgaagaagca ggtatcgatc aggtcatatt tgatcctcat
     4321 aggagcaggg ttataatcga ggcagcaaag ccaggactag ctataggaaa acaaggcgcc
     4381 ttcttaagtg agataaggac taaaacatta tgggtgcctc tgataagaag aaagccagct
     4441 attcgctcaa agcttatcga aaacatcaga gcagtcctat acaaagaatc agattacaga
     4501 cgaaagtttc ttgacaagac cggtcacagg atctatgacg gctggatgag ggaaaagaag
     4561 cacgagtggg taagggtaag ctatcttggc ggcggaaggc aggttggaag aagctgcttg
     4621 ttcttgcaga ccccagaaag cagagtctta cttgattgcg gtgtagatgt ggcttcatca
     4681 gaccaggaag catatccact tttagaagcc cctgagttca acatccagga gcttgatgca
     4741 gtcatcattg cacattcaca cttagaccac agcggttttg taccatattt atacaagtac
     4801 ggatttagag gacctatcta ctgtacagag ccgacaagag atgtcatggc tctccttgct
     4861 cttgactatg tcaagataat gaggggagag ggcaaagagc ctatctattc tacagacgat
     4921 atcaaagaga tggttaaaca cactataaca ttagaatatg atgaagtgac agacatcact
     4981 ccagatatca ggatcacgtt gtataacgca ggccatatcc ttggctcagc gatgatccat
     5041 ttgcatattg gaaatgggct tcacaacctt ttatacacat cagacatgaa gtatggaagg
     5101 acaaggcttc ttgagcctgc tgtgacagat ttcccaaggc ttgagacact gatgatagaa
     5161 gggacatacg gcggaaaaga taatctgctg cctccaagac aggaacaaga agagctgatc
     5221 agaagaaaga tagacgagac tataaaaaga ggaggtaaag tgctcatccc tgtccttggt
     5281 tcaggaagag cgcaggaagt gatggtgctg cttgaatcat ttatcaggaa cggacagctg
     5341 gataaagtgc ccattgtcgt cgatggcatg gtatgggaca taactgccat ccatactgcc
     5401 taccctgagt tcctgaatag tgttgtaaga aaacttattt tccataaaga ccaaaatcca
     5461 ttcctctcag aggttttcag agagataggc tcgcaaaaag aaaggatgca ggtcatcgaa
     5521 gagatgggtc cctgcgtcat tttggcaaca tcaggtatgc ttatgggggg gccttctgtt
     5581 gaatatctaa ggcatcttgc agataatcca aacaattgct tgctttttgt cgcttatcag
     5641 ggcgagggct cactgggaag aaagatccaa cgaggaaata aagagataat gtttggcacc
     5701 gcgatgaggc cagagatatg ccccgtaagg atggaagtgg tgactattga aggatattcc
     5761 ggccattcag gaagaaaaga gctgatgaca ttcatgctta agtgcaatcc aaagccaaag
     5821 aaagttatag tcaaccacgg cgaaaactca agatgccttg atctagcctc ttctatacat
     5881 aagcagaaca gggtagagac agtctgcccg agaaatctgg aagttattag gataaaataa
     5941 cacccttccc acttaattcc gtgggggtag aaatttcctt tcttgtgtat tatatgtttg
     6001 cccttctctc ttgcttattg acttcctagg gcggttggta tggggctctg ctgttttttg
     6061 gcagaaatat agaatcagtg ttagggctgt tgcagcaaac catagctttt tcataggtaa
     6121 aatgattata tccgtgatta tttaggcgtt ttctaagata gatttataat tgactttaca
     6181 atccaatcgc ctatgcaaga cacatatgcc aacctggcac agatgatagg gatgatcaag
     6241 acatcaagaa accttgggta tcacaacgaa gcgccagaac tatctgtcaa gcttttttct
     6301 ggatgcagat ctattgagga attggcggca aaagccaagc ttcaaggcga tgagcagacg
     6361 aagttaaggc acggctgtgc gttgttatac aagacagctg aattgatgat gggctcacca
     6421 gatgcaaggg tttaccttga ttgtcttatc tccaatgaac ctgggctgag cagccttcat
     6481 gctcatgcag caatggttgg aatcgattat gcagcgcaat gtctggaaaa taaggccctt
     6541 cttactccaa aagagttgga taagctcgca tttgataagt gtgcaaaaag atatgataac
     6601 gtagcattct acaacatgcc ttcagttgct attgtgcatg acagggggac agtagtagat
     6661 atccatgaga ttaaagatga aacccgtatg aaggagataa acgctcctaa gatcggatat
     6721 ttggagggcg gttttttgat catcccccac tcagaaccat tcacagggac agctcaggaa
     6781 gcggtagatc atttgcataa aagagggcag cctaatttta tattgacaac tgaggttatg
     6841 cttgcacatt acatcgctaa cagatatact cttatgcggc tgtgggaggc ttttaagaca
     6901 tctaagattc cgctggatga cttgagtaaa cttacaaaag caggagtcat ttttgataat
     6961 ggttgatcat tatatcaggc ttattttctt tactcctgta tcctaacaaa actatttata
     7021 gacgggtaaa ttagagagca atagcggttt ttatggagaa tgaagagctc gaagtaagaa
     7081 aagtagcttg ccacacctgc ggtaagttat ttgaagtagt tgttcccaaa gattacatga
     7141 cgggcgatat ctttttctgc gaaggatgct caggcattaa gatttttgag tttaaggtag
     7201 atggcctgcc gccgataaag gacggcgcaa attcaatgtg gaacaagcca gagatggctg
     7261 aaagaatcag ggcgttgaga gttggggcaa aggctgcact gaacggatat gtattgaaga
     7321 atgatatcac ccttgctgtc gaaatcgagt gtatggggga ttgtggagac cttgataact
     7381 ttgtcgcggg aatatgtgat tgcctgcaga ttgcaaatac aaagccccaa tttattcatc
     7441 cgtcgttaag aaacgaccca agcatagatc cagcaacggt ctgctttatt gaggatgatt
     7501 caaaaattat gcggatagag tcaagcataa ataagaaagc tagtaaaaat ggctatctta
     7561 ttgagctgta cgacgagaaa taaaggcaga gaagggggtt gtttcattag ctcttagaaa
     7621 aacaacggtc cctgccccag gcaagggtca agatgttaaa gggttactca attccttgct
     7681 caaaaaggtg tataccagta agggtttaaa atacttatta aagacaaatc atagcttctt
     7741 ctgctgttca attcttgtct ctgtcacggg cttatcatcc ttgtgccagc cggaataata
     7801 ctgaagcctt ttttccttcc ttaagccaaa tggagctctc ttctttttta cttcctttag
     7861 cttttcttcc tttcttaatt cttccaaagc ctgcagcaaa ggattagtca ttcttttctt
     7921 ccaaaatctt atagccgcat ttcatgcatc taagctcttt ctgcccaagg cttatcttgt
     7981 ttacctcatt gcacatcggg catactttaa ttcctgtttc ttctggcata aacataagaa
     8041 agggcgcatc ttataagtat ctttactttt tgtaccgata gaagtaaatc cttctttttg
     8101 ttttgctgcc aggcattaat gcgagcttgc aaatttataa ttatgcggta ttaatggatg
     8161 acagaattca agaattatag taaattaaat tgaatatttc tcattaaatg gaataagaga
     8221 tagaactttt tgtttgttgt accaaagact tctgaaga
//
LOCUS       DASRUK010000019         3924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2030150, whole genome shotgun sequence.
ACCESSION   DASRUK010000019 DASRUK010000000
VERSION     DASRUK010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2030150"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VFF28_00780"
     CDS             complement(<1..332)
                     /locus_tag="VFF28_00780"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZX44195.1"
                     /translation="MSLDSKDQLIIESLKLDCRAPIRDIAKRTKLRPSTVHQRINRLK
                     ESGVIERFTIKLNNEAVGEGFIVFMLIKTKPAAMLDKIVTDSHVKEFFGVTGEYDLLL
                     KLKFKDVS"
     gene            468..1889
                     /gene="ahcY"
                     /locus_tag="VFF28_00785"
     CDS             468..1889
                     /gene="ahcY"
                     /locus_tag="VFF28_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013010732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HZX44196.1"
                     /translation="MDKTQNYEVKDLKLADFGRKEIEIAEKEMPGLMATRKKYKAQQP
                     LKGARITGSLHMTIQTAVLIETLADLGAEVRWASCNIFSTQDHAAAAIAKAGIPVFAW
                     KGETLKEYWYCTEKALDFGDGKGPTLIVDDGGDATLMIHKGVEIEKDPSILDKDYSSE
                     GEDFRELMISLKELYAKDKQRWTKAAKEIKGVSEETTTGVHRLYQMLEEKKLLFPAMN
                     VNDSVTKSKFDNIYGCRHSLTDGIKRAMDVLISGKTAMVCGYGDVGKGSAESLKNEKA
                     IVMVSEIDPICALQACMAGYSVCTVEDALPKADIYVTTTGNCDIITTDHMSRMKDQAI
                     VCNIGHFDNEIQVSALEQWPGIKKTEIKGSECPVDRYAFPDGHSIYVLAKGRLVNLSC
                     GTGHPSFVMSNSFTNQVLAQIDLWTKKREIRVYMLPKELDEEVARLHLEKLGAKLTKL
                     SEKQSNYIGVKKDGPYKPSFYRY"
     gene            1925..2785
                     /gene="amrB"
                     /locus_tag="VFF28_00790"
     CDS             1925..2785
                     /gene="amrB"
                     /locus_tag="VFF28_00790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HZX44197.1"
                     /translation="MVRNPVVAGMFYAESPEELENQLKESFLSKFGPGSLPENKRTKS
                     IFGIISPHAGYQFSGPGAAWVYKELAESKFPEVYILLGLSHSGFPSCVSLEDWQTPLG
                     TVKVDEQFQRVLMANSGLKADEKAHQAEHSIEVQLPFLQFISKDNQEKLRIAPIIVSS
                     DYEKIAAAIAKTIEQTMKKVVIIASSDFTHYGVNYGFFPFSSKPKENMYALDKKAIEK
                     LEKLDEKEFLDYISKTGATICGAIPIAAAIKTLKLIGAKKAKLLSYYTSGDVTDEYSS
                     AVGYASIKME"
     gene            complement(2788..3618)
                     /locus_tag="VFF28_00795"
     CDS             complement(2788..3618)
                     /locus_tag="VFF28_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HZX44198.1"
                     /translation="MVDNNALVVFQDKKIRRIWHNNEWYFSVIDVVNALDASTIPKRY
                     WSDLKPKLSSEGFEPYGKIVQLKLPAEDGKLRETDCASTKNMFRIIQSIPSPKAEPFK
                     QWLAQVGYERTQEIQDPELTQARMKELYRAKGYSDEWIEKRVRGIAVRQELTDEWKKR
                     GVEENREYSILTNEISKATFGKTVEEYKEFKGLKKENLRDHMDDLELIFTMLGEASTT
                     KIAKSKDAQGFFENKAAAQKGGSISGDARRKLEIESGSKVVTPENFLTKPEKDKKRLA
                     "
BASE COUNT         1230 a          819 c          785 g         1090 t
ORIGIN      
        1 tccgaaacat ccttgaattt taatttgagc agcaggtcat actctcctgt cacgccaaaa
       61 aactccttca catggctgtc ggttactatc ttatcaagca tagcagcagg ctttgtcttg
      121 ataagcatga agacgataaa tccctcgcct acagcttcat tgttaagctt gattgtaaac
      181 cgctcgataa ccccgctttc cttgagacgg tttatccttt gatgcactgt gcttggccta
      241 agctttgtcc tcttggcaat atcccttata ggagctctgc agtcaagctt caaggactct
      301 attatcagct ggtccttgct gtctaagctc attgagaggt atctgtccag ctactatata
      361 aattttccgt tatttgtcca aaaaatcaag gaattaatag acaaaacata ccaaaccaat
      421 aaaaaaatat ataaattatc taacaatttt aggcactagg tgataatatg gataaaacac
      481 aaaattatga ggttaaagac cttaagctcg cagattttgg aagaaaagag atagagatag
      541 cagaaaaaga gatgcctggc ctcatggcta caagaaagaa atacaaagca cagcaaccct
      601 tgaaaggtgc aaggattaca ggaagccttc acatgacgat tcagacagct gtcctgatag
      661 aaactctcgc cgatcttggc gctgaggtaa gatgggcaag ctgcaacatc ttctcgacac
      721 aagaccatgc agccgcagcg atagcaaaag caggaatccc tgtctttgca tggaaaggcg
      781 agaccctaaa ggaatactgg tattgtacag agaaagcctt ggattttggt gatggcaaag
      841 gccccacatt gatagtagat gacggcggcg atgcaacttt gatgattcac aaaggcgttg
      901 agatagaaaa agacccttcc attttggaca aggattatag tagtgaagga gaagatttta
      961 gagagcttat gatttctcta aaagaactct atgcaaaaga caagcaaaga tggacaaaag
     1021 cagcaaaaga gataaaagga gtttcagaag agacgacgac aggagtgcat aggctctatc
     1081 agatgcttga ggaaaaaaag cttttgtttc cagctatgaa cgtaaatgat tctgtaacaa
     1141 aatcaaaatt cgacaatatt tacggctgca ggcatagtct taccgacggc ataaaaaggg
     1201 ccatggatgt ccttatctca ggcaagacag cgatggtatg cggctatggc gatgtaggaa
     1261 aaggaagcgc ggagtcattg aaaaatgaaa aagcgatagt catggtctct gagatagacc
     1321 cgatatgcgc tttgcaggcc tgcatggcag gatatagtgt ctgcacagtc gaagacgctc
     1381 ttccaaaagc agacatctat gttacaacaa caggtaattg tgatataatt acaacagatc
     1441 atatgagcag gatgaaagat caggcaattg tatgcaacat aggccacttt gataacgaga
     1501 tacaagtttc agctcttgaa caatggccag gaataaaaaa gacagagata aaaggaagcg
     1561 aatgccctgt cgacagatac gcatttccag atggtcattc aatctatgtc ctggcgaagg
     1621 gaaggcttgt gaatctttca tgtgggacag gacatccaag ctttgtcatg agcaactctt
     1681 ttacaaatca ggttttggcc cagattgacc tatggacaaa aaagcgagag atcagagtat
     1741 acatgctgcc aaaagagctt gacgaagagg ttgcaaggct tcacttagaa aagttaggcg
     1801 caaagctcac gaagctttct gaaaagcagt caaattatat cggcgtaaaa aaagacggcc
     1861 catacaagcc ctctttctac agatattaag aaaatttttt atatttctta tatttagtct
     1921 tcttatggta agaaacccag tagtagcagg catgttctat gcagaaagcc ctgaagagtt
     1981 ggaaaaccag ttaaaggaaa gctttctatc aaaattcggc cctggctcgc tgccggaaaa
     2041 caagcgcaca aaaagtattt ttggcataat ctcacctcat gcaggctatc agttctcagg
     2101 tccaggagca gcctgggtat acaaagagtt ggcagagtca aagttcccag aagtttacat
     2161 cctattgggt ctgtctcact caggctttcc ttcctgcgta tcattagaag actggcagac
     2221 ccctcttggc acagtaaagg tagatgaaca gttccaaagg gttctgatgg caaattcagg
     2281 cctaaaagca gacgaaaaag cccaccaggc agagcattcc attgaagtcc aactcccttt
     2341 tctacaattt atctccaaag acaaccaaga aaaactaagg atagcaccga tcatagtttc
     2401 cagcgattat gaaaagatag cagcagccat agcaaagaca atagagcaga ccatgaaaaa
     2461 ggtagtgatt atagcatctt ctgatttcac ccactatggt gttaattacg gcttttttcc
     2521 tttttcaagc aagccaaagg agaatatgta tgcccttgac aaaaaagcta tagaaaagct
     2581 tgaaaaactg gatgagaagg aattccttga ttacatatca aaaacaggag cgactatctg
     2641 cggtgcaata cctatagccg cagcgataaa aaccctaaaa ctcatcggcg caaagaaagc
     2701 aaagcttttg agttactata catctggaga tgtcacagac gaatacagct ctgctgttgg
     2761 atatgcatca ataaagatgg aatagattca tgccagcctt tttttatcct tttctggctt
     2821 tgtaaggaaa ttttctggcg taaccacttt tgatcctgac tcaatctcaa gctttcttct
     2881 tgcatcccct gagatgctgc cgcccttctg tgcagcagct ttgttctcaa aaaaaccctg
     2941 tgcatccttg cttttagcta tcttcgttgt tgaggcttcg ccaagcatag taaatatcaa
     3001 ctctaagtca tccatatggt ctctaaggtt ttctttcttt aaacccttga attctttgta
     3061 ttcctctaca gtcttgccga aagttgcctt gcttatctca ttggtcagta ttgaatattc
     3121 cctgttttcc tcaactcctc ttttcttcca ttcatctgtg agttcctgcc tgacagcaat
     3181 gcctctaacc ctcttttcaa tccattcatc agaatagcct ttggccctat acaactcctt
     3241 cattcttgcc tgggtaagct ctggatcttg aatttcctgg gtgcgctcat agccgacctg
     3301 tgcaagccac tgcttaaagg gctcagcttt tggtgaaggt atagattgga tgatacgaaa
     3361 catattcttt gtacttgcac aatcggtctc tcttaatttt ccatcttcag cgggcaattt
     3421 caactgtacg attttaccgt acggttcaaa tccctctgat gaaagtttgg gttttaggtc
     3481 ggaccaatac cttttaggaa tggtgcttgc atctaaggcg tttacaacat caataactga
     3541 gaaataccat tcattattat gccaaattct tcttatcttt ttgtcttgga agacaaccaa
     3601 agcattatta tccaccattt tgttcatctc tccctttcct ataagtataa agcgatatta
     3661 ttaaaaactt tcttttgagc atcttctgat ttcacccact atggtgttaa ttatggcttt
     3721 tttccttttt caagcaagcc taaggagaat atgtataccc taaaaaagcc atagaaaagc
     3781 ttgaaaccta atctcgccat ttgaataaat ttaatagggt cacgtttgtt ttatattttt
     3841 gtgaaaacca taaaacaaaa cgtaacccta agcatcggta gtttagcact aatagaaaaa
     3901 gttgacggtc aatatcaata tttt
//
LOCUS       DASRUK010000020         3095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1028040, whole genome shotgun sequence.
ACCESSION   DASRUK010000020 DASRUK010000000
VERSION     DASRUK010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028040"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..736
                     /gene="fabZ"
                     /locus_tag="VFF28_00800"
     CDS             236..736
                     /gene="fabZ"
                     /locus_tag="VFF28_00800"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF000582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HZX44199.1"
                     /translation="MKTIDQLKNEKGEIDSNAIKQIIPYDQPFLMIDRVISIEKNKVV
                     AFKNTTANESFFKGHFAGFPIMPGALIIEGMGQAATLLLRYNLDNHWEKEVLAYKIKE
                     AKFSAPTFPGDQVRYEVTLLGKDERGALMSGAALVDGNKVAEATLMLAIVDRREFRGK
                     ASPAFK"
     gene            745..1869
                     /locus_tag="VFF28_00805"
     CDS             745..1869
                     /locus_tag="VFF28_00805"
                     /inference="COORDINATES: protein motif:HMM:NF014820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZX44200.1"
                     /translation="MYISGVGMTRFSVEDRNTWQMAYEAAYEAVNDSIVGLDKIDAIV
                     VSTVDTIVNPERQRQYQALISSLFKKKIPIIRVPAVCGGGGAALWTALRLGFDNVMVL
                     STDKIATNTTPIIVNEIMNATDRVWEQEEGVNFIAENALVAQEHMRKYGTTTDDLALV
                     AFKNHENAFLNPKTPFYKKKVSLEEIKSSPVLAAPLRLYDCTMNVNGAAAVILTKDKT
                     DVCIRGSGFCTDFLPPFEREEMSTWMGTVAASKYAYEQAGLGPSDIDIAEVHDAFSIL
                     EIISYEDLGFCKKGEGMKMIRDGMTQLDGRLPVNTSGGLKARGHPISPTGLAMIYELT
                     KQLRGECGERQASNVKKGLAQNIGGAGGSVSCHILTKVGG"
     gene            1872..2990
                     /locus_tag="VFF28_00810"
     CDS             1872..2990
                     /locus_tag="VFF28_00810"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HZX44201.1"
                     /translation="MILRWVCKNCEKKWLYPIEKCIYCKGDIIKEKGKSMKVVGTTKV
                     NIPCPPHPIVPYNVLILEDELGNRMPKKTMRDYKIGDEYKRLGSDDESAVAIVKIKYD
                     YFEAVAEAIELIRFNVKDEKILIKPNIFAGAYPYQGLTTNPKVVKGLLDYLLSKGVKA
                     DNITIAEQVQFGDALKAFKRAGFYDLAQEYKVNLVDIGKGEFVEKEQDGFKFKVSKEV
                     IDSKLIINVPVMKTHLLLGISGALENMTRVVSKESYKEIEKDMERAIKGIGVLNKALG
                     KYLTVCDATIGLQGNGPGEYGEPAFMNMVFASRDPVALDKIFTEAGLLRDSKHVIAAG
                     DIGVGNPDLVDITVVGEELDACKRELKPAIGSKLIKLR"
BASE COUNT          982 a          588 c          775 g          750 t
ORIGIN      
        1 aggctcacca tctcaaaagg ggaaagattt atgttactca gaatccaagg ggtttatccc
       61 cttttatgcc ccaagggcat caatgcaata gattattaga aagaggcttc agccgttagg
      121 ctggagagtt tcacttccgc aatatggcgg ctttaaatcc ctaatcctgg tttaaagcac
      181 aagcttttta aatcgtttta gcatccttta gtttagacca tacaggggtg tatatatgaa
      241 aaccatagat caactgaaaa atgaaaaggg cgagatagat agcaacgcca ttaaacagat
      301 catcccttat gaccagcctt ttttgatgat agatagagta ataagcatcg aaaagaacaa
      361 agtggtggct tttaaaaata ccacagcaaa tgaaagtttc tttaagggtc attttgcagg
      421 atttccaata atgcctggag cattgataat cgaaggcatg gggcaggcag caacacttct
      481 acttcgctat aacctagaca accattggga gaaagaggta cttgcctata agataaaaga
      541 ggctaaattc tctgcaccca cttttcctgg cgaccaggtg agatatgagg taacgctgct
      601 tgggaaagat gagagggggg ctttgatgag cggagcagca cttgtcgacg gcaacaaggt
      661 ggctgaggct acactgatgc ttgctatcgt cgataggaga gagtttaggg gaaaggcatc
      721 tcctgctttt aagtgagcct tcccatgtac atcagcggcg taggtatgac ccggttttct
      781 gtagaagata gaaatacctg gcagatggca tatgaggctg cctatgaagc tgtgaatgat
      841 tctattgtcg gacttgacaa gatagatgca atagtagtct ccactgtgga taccatagtc
      901 aatcctgaga ggcagaggca ataccaagcg ttgataagct cactttttaa gaaaaagatc
      961 cccataataa gggttcctgc tgtctgtggc ggaggaggag ctgctctttg gactgcacta
     1021 cggctcgggt ttgataacgt aatggtacta agcactgata aaattgcgac aaacacaaca
     1081 cctatcatcg taaatgagat aatgaacgct acagatcgcg tttgggagca ggaggaggga
     1141 gtcaatttta tcgctgaaaa tgcacttgtt gcgcaggaac acatgaggaa gtatggaaca
     1201 acaacagatg accttgcact tgtcgctttc aagaaccatg agaatgcttt cctcaatcca
     1261 aaaacccctt tctataagaa aaaggtcagt cttgaagaga taaagagcag ccctgtgctt
     1321 gctgccccgc ttagacttta tgactgcacc atgaacgtca atggagctgc cgctgtcatc
     1381 ttgaccaaag ataagacaga tgtctgcatc agaggaagcg gattttgcac tgactttttg
     1441 cctcccttcg aaagggaaga gatgagcact tggatgggaa cagttgctgc aagcaagtat
     1501 gcatacgagc aggcaggcct tggtccaagc gatatagata ttgctgaggt ccatgatgcg
     1561 ttcagcatcc ttgaaataat ctcgtatgag gatcttgggt tctgcaagaa aggagagggc
     1621 atgaagatga taagagacgg gatgacccag cttgacggca ggcttcctgt caacacctct
     1681 ggcggactca aggcaagagg gcatccaatc tctcctacag gccttgcaat gatctacgaa
     1741 ctgactaaac agcttagagg agagtgcgga gaaaggcaag cttctaatgt caagaaagga
     1801 cttgcccaga acatcggcgg agcaggcgga agcgtttcct gccatatact tacaaaggta
     1861 ggtggttaaa tatgatacta agatgggtat gcaaaaattg cgaaaaaaaa tggctttatc
     1921 ctatagaaaa gtgcatttat tgcaaaggtg atataatcaa agagaaaggg aaaagcatga
     1981 aagtggtcgg aacaacaaag gtaaacattc cctgcccacc acaccccata gtgccctata
     2041 atgtactgat attagaagat gaactgggca atcgtatgcc taagaagaca atgagagatt
     2101 ataagatagg ggatgaatac aagagacttg gaagcgacga tgaaagtgca gtggctatcg
     2161 ttaagataaa atatgattat tttgaggctg tcgctgaggc aatcgagctg ataagattta
     2221 atgtcaagga tgagaagata ctgattaagc caaatatctt tgcaggcgca tatccttatc
     2281 aggggcttac caccaatcct aaggtggtaa agggactact cgactatctg ctttcaaagg
     2341 gagtcaaagc tgataacata actatagctg agcaggtaca gttcggcgat gctctaaaag
     2401 cattcaaaag agcaggattt tatgaccttg cccaagagta caaagttaac cttgtggaca
     2461 taggcaaagg agagtttgtg gagaaagagc aggacggatt taagtttaag gtaagcaaag
     2521 aagtcatcga tagcaagctt atcatcaatg tgcctgtcat gaagactcat ctgctgcttg
     2581 ggatatcagg ggctcttgaa aatatgacaa gggtcgtttc caaggaaagt tataaagaga
     2641 ttgaaaagga catggagcga gcaataaaag gaataggggt ccttaacaaa gctcttggaa
     2701 aataccttac ggtctgtgat gctaccatag ggcttcaggg aaacgggcct ggtgaatacg
     2761 gcgagcctgc tttcatgaat atggtctttg catcaagaga tccagttgcg cttgacaaga
     2821 tatttacaga ggcaggactt cttcgagatt caaaacatgt gatagctgca ggagatatag
     2881 gtgttggaaa tcctgatcta gtagacatta cagtcgtagg agaagagcta gatgcatgca
     2941 agagggagct taaacctgcg ataggctcaa agcttatcaa actacggtga aacaaatgtt
     3001 catcaagggc gtcggaagca ctaactataa tgtgcatgaa aagaaaacac atcttcttgc
     3061 ctatgaggct gctcttgact gccttaatga cgccg
//
LOCUS       DASRUK010000021        12402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_360904, whole genome shotgun sequence.
ACCESSION   DASRUK010000021 DASRUK010000000
VERSION     DASRUK010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 12402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12402
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360904"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1263)
                     /locus_tag="VFF28_00815"
     CDS             complement(<1..1263)
                     /locus_tag="VFF28_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44202.1"
                     /translation="MLFNKCIALFLIFLICSISVAVSEETDDKTFIFPKYPEQPTHSD
                     FAAASNECPPLCPIGYLDEGVSCIGPDCTRICSIMACSDSGSIVFNDYSNRMPEWNDR
                     TYDYEDEGPGFTLQSGKCYRLDYISSKSTDSSDSSECYDLYTDDDYKSEDYDMDCKGE
                     DIDAYAGIGFVGSKKSGTWLRTIPGSTQNTDYGYIGKVDNSYWKYRYITYYGRDASPD
                     NSGSDAGSWDSFNNEICDEAGTQTVYCAPSYESCALWGKNKCGYNCAGETTRPFIVMG
                     VYGGDNNDLGDALDDNWNCGDIDDNNFFAQSNNYQVTEYNLEKKYSNTQAQCCISHHT
                     SNCFDDNTYWYDSCGKLEDKKEDCQKFCTPWGSNYCKENGVYHQRTCYDTGCSAGSCY
                     ANPYTDEDKLASCTQGCSGGSCISCTPNN"
     gene            1370..1657
                     /locus_tag="VFF28_00820"
     CDS             1370..1657
                     /locus_tag="VFF28_00820"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZX44203.1"
                     /translation="MLGKDFKGVLGCDGWSTYFVYAKDTGILLQRCWAHALRELKEIC
                     YNKKRQDKSLVKALHDIFEKVKEARTSREIREQFYMELVGVLMMELPPTSL"
     gene            complement(1706..2380)
                     /locus_tag="VFF28_00825"
     CDS             complement(1706..2380)
                     /locus_tag="VFF28_00825"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZX44204.1"
                     /translation="MGKEKFMLVSLKESKAKELAQVITNDSCRKILDYLSEKEDSTES
                     VMADELKLPISTVHYNLQLLIKSGIVVSEEYHYSAKGKEVNHYQLANKYIIIAPKSIH
                     GIKEKLRSILPAGLIAAAGAGLLHWYSTYYSASAKILAAAPLMQSAKGAASAPFVESV
                     RDEAVQAITPSIVEKSARAAIEKAPEAVVNVTTSDTSIALWFLIGAVFVIVLLVFIEM
                     IRNRKN"
     gene            complement(2387..3094)
                     /locus_tag="VFF28_00830"
     CDS             complement(2387..3094)
                     /locus_tag="VFF28_00830"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HZX44205.1"
                     /translation="MKIKYTIGAVLVLAILALTGCQTKDGNTINVSGQSELLAKPDEA
                     EVWAGISIVKDTADEAQNEANKVINDIVDGLKAKGINEKDIQTESLNLYEERTWTENE
                     GSKLEGWRATQTLKVKTTDLSKVGDIVDAAVDNGANQIQNINFQLSDAKEAEYREQAI
                     TKATQDAKSKAETIARSLGVSLGKLKTVSEANYYAQPYFYAMRAGDGSAAVAEAAKVM
                     PKDVTVNANINLVYYVK"
     gene            complement(3122..3259)
                     /locus_tag="VFF28_00835"
     CDS             complement(3122..3259)
                     /locus_tag="VFF28_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44206.1"
                     /translation="MSKGEERIERGYRQGEAVGLIPREGLAKNSFNWDKTYSKLYINA
                     L"
     gene            3390..3548
                     /locus_tag="VFF28_00840"
     CDS             3390..3548
                     /locus_tag="VFF28_00840"
                     /inference="COORDINATES: protein motif:HMM:NF014009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HZX44207.1"
                     /translation="MNMVELTSSHKHELIDITYKVKAIVQSSKVKEGICQIFVPHATA
                     AITIKRER"
     gene            3697..3777
                     /locus_tag="VFF28_00845"
     CDS             3697..3777
                     /locus_tag="VFF28_00845"
                     /inference="COORDINATES: protein motif:HMM:NF014009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44208.1"
                     /translation="MQLGTWQDIFFCEFDGRKKKESYHKN"
     gene            3870..4268
                     /locus_tag="VFF28_00850"
     CDS             3870..4268
                     /locus_tag="VFF28_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44209.1"
                     /translation="MNICKHLERLKESLEVIDESITRGIIRRQRTIGFSLSAASADMF
                     EIFLHKEKLVDPGFVVKHEWFKSKNKLEEKFPFDFAAKAVIFELMGLMEEKRDALCYG
                     VPKEEKYIRDAIDNFNELKKIFDTKGIIHE"
     gene            4261..4869
                     /locus_tag="VFF28_00855"
     CDS             4261..4869
                     /locus_tag="VFF28_00855"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZX44210.1"
                     /translation="MSKFLSYAVDFASFLLEKVDSAKISSIILFGSVARNEASLESDI
                     DIFIEVAGSFDLKKQIENARLDFFASARFNDYWRLKGINNDINVLAGKVEEWKDIKNS
                     IISNGVVLYGKYESVPRGAVHKTIFSWEKIKPESRRVLLHKKLFGYKAGEKRYPGLAE
                     KYEGERVGKGSIMVPARHTNVFMEVFREMKIAVKIRKIVEYS"
     gene            4878..5942
                     /locus_tag="VFF28_00860"
     CDS             4878..5942
                     /locus_tag="VFF28_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44211.1"
                     /translation="MQKKGVTDVHFNWIFILIAGAVIFMFFITIVNKQRAFSEVKVAQ
                     SLVVSLDAILTGAEVSTRTVNIIKDLPKADIGFECNNYFVGGIPRDTGGSVIFAPSVL
                     KGRRIIAWAVDWKMPFKVANFLYLTHPEVRYIIVNDTSSSANLAQKLFDELPKELNKE
                     IVAKDKIGDIIDKNNYKVKFIFFNDNIQPPGVPKLKDMPFKDVTAISLPASGIIASSG
                     ELSFWEKNKVGAFEWIDKGDSFYIEKESLYGAIFAENKDTYDCVMRKAFDKLSYVSRV
                     YRSRSSELGTFYRAYPPKQYCANYHVTTEFDNTLVPLSTQLAQSFPTTMPEITSLKEA
                     ADNIEQQNQKASLKTCALVY"
     gene            5944..6429
                     /locus_tag="VFF28_00865"
     CDS             5944..6429
                     /locus_tag="VFF28_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44212.1"
                     /translation="MKKSQLQMGESVAVLFIFFILIAFGFSFYMNIMKGSQKIDLKEN
                     AELKAISIAQRASFFPELECSEEIVKEGCVDVYKLDAAADIIDDSNVVYFEMFGYSLI
                     RAVQLFPEEEKVWVIYNNTLPNYESRLTSFIPVSIYNATMKRYTFGMLAVDVFSGVVE
                     E"
     gene            6426..6911
                     /locus_tag="VFF28_00870"
     CDS             6426..6911
                     /locus_tag="VFF28_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44213.1"
                     /translation="MKRSQMEIMGLAIIVILISIGMLFAIKFVVFKKPEPFKSEYTKT
                     ELASNILTSLMRTTMEGCSDLSMEDIYQACGKDPDNPPVVCSPAEDACQFLNRTTRRI
                     LNLTLVEWNKGYELHASTVTDSIIDVGRCPGAKKQKPYTIPIDASSKNSLKITLDVCD
                     K"
     gene            7021..7272
                     /locus_tag="VFF28_00875"
     CDS             7021..7272
                     /locus_tag="VFF28_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44214.1"
                     /translation="MKKGQSLSLNTIIIAAIVLIVLIVLWAIFTGRMGKFTTDLAKEG
                     EVKTCEKMGGVGPQEPPCDTNKKPVISSDTTGTTVCCVP"
     gene            7269..7634
                     /locus_tag="VFF28_00880"
     CDS             7269..7634
                     /locus_tag="VFF28_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44215.1"
                     /translation="MKKAQSLSLNTIIIAAIVLIVLIVLWAIFTGRMGKFTSGVNEQD
                     LQSQAAAANVGNTECDGKCFTTVGTKDGKWELMKVKGEKASACSTSQKSVGTAFSDLE
                     KGQVCCISKTVLSSNPACS"
     gene            7636..7779
                     /locus_tag="VFF28_00885"
     CDS             7636..7779
                     /locus_tag="VFF28_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44216.1"
                     /translation="MLKSLNGDTILKLSFLFILLILLGYLIYKLFIIDETINLMINNR
                     TFG"
     gene            8024..8953
                     /locus_tag="VFF28_00890"
     CDS             8024..8953
                     /locus_tag="VFF28_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44217.1"
                     /translation="MSDRKGKMMEIMLGGIVIVVTLVVLIQVLLVLIGAGKAKGAEQL
                     CENFNQARLAGQVEIKDYPVFNLVKRAPCKKQSLKLPLKDSDLEGTMDDIGLHIQKCW
                     KQWLEGRTDRISELLSSYKECVTCYSFEISKGIPTFQGSDLKKYLSDNVQIVRDTSDR
                     CNINGGGYCIEEESCTAGGGTKVPSSSCSTGEICCNKANECENKGGYCRATCDTSDAE
                     YAGWNCKEGRCCVEGQNMQSYEGVLNSNQGRILVAVDNFIPVSESYSITFSTVNGPWS
                     DFKERLPNFGFPEEKANSIIISKAKDTEGLCDE"
     gene            8946..9086
                     /locus_tag="VFF28_00895"
     CDS             8946..9086
                     /locus_tag="VFF28_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44218.1"
                     /translation="MNKKAILLFWEKLVWWAAMILITFIVLALIFKSKGAMTDIIGYI
                     FR"
     gene            9083..9757
                     /locus_tag="VFF28_00900"
     CDS             9083..9757
                     /locus_tag="VFF28_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44219.1"
                     /translation="MKKAYTFIEIIVFMAVIAVAVFIALAVFSPRFQKIGKIILGYEL
                     PGKPDEGFSGKEEVTEKVQAAFDGFYGALVKAKNTNECYMKYPSLNLGTNVLEVEYIE
                     RMKGTNLRLMNEKGIRIAEYFVDGLQPCIALPDKKRQIELEQSISNINIKGNNKAIFW
                     NDEYSLFPKYPFLHIIDKKFCFFTDSSIISPQGDVYGERTITRAFTAGFNEKDMTKII
                     KPICGG"
     gene            9759..10037
                     /locus_tag="VFF28_00905"
     CDS             9759..10037
                     /locus_tag="VFF28_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44220.1"
                     /translation="MNKKALEISISILVIALIALASAVIALAIYKVTIGKEAKVTDSL
                     IADKDQDKIIDMSDKCPNKPGIIEYAGCPSKDKLTECENSKEPNAENC"
     gene            10021..10485
                     /locus_tag="VFF28_00910"
     CDS             10021..10485
                     /locus_tag="VFF28_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44221.1"
                     /translation="MQKIVKKRRGFTWGLEGTARLIYVALGLIFLLALLKGAFKYTGD
                     AEKSFDDLASLLNSLEDKDTKPLQLTIDKSSYFLFFNKGFDLRYALTGYGGMGVTLKD
                     SITIKRPQQCPAGTACLVWCGKLTDEAGDIKTCSKVKKQVPINKDKSKAIKF"
     gene            10618..11007
                     /locus_tag="VFF28_00915"
     CDS             10618..11007
                     /locus_tag="VFF28_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44222.1"
                     /translation="MCDKKPCFSDEEINKRISLAQEEIVKKNFEFAIKSLIGVDANSD
                     GNIETFFYINDLVKDDFGSNYYKYKFGYKTEDPSQAAFLETVQEKEKKEFELKIRSYA
                     AAGKIVNIDEGNSGSIAKFSSDRELKV"
     gene            11009..11935
                     /locus_tag="VFF28_00920"
     CDS             11009..11935
                     /locus_tag="VFF28_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44223.1"
                     /translation="MPKKGMAIRELSNFIIGAFVFITLIGIGVSYGKGEAVFKVQLAK
                     DIGLMIEQMYNTPSSMNIYETYPEDVSSFNIKVKGKTITVFSKEGDSTKAEYNFTLNP
                     SYPELDILLKKPQRMYIGNIAGKITITDKAPDLRKLKCSNAEIEVKNILIDSGLEISG
                     QKDKSQAANSVAYSVVSRLEGNYDIEHARNDYIDEMVGTALPFIPSKFEAKVKDTDLI
                     IGFRANEKEDLVVSYWPDSKKSNEAESLACRIVNGFIEKGLIKEGYGVSVAMAQTDIL
                     RNDKVSILVEFGDKIVKPVDMGEVIFDAIKDS"
     gene            11916..12395
                     /locus_tag="VFF28_00925"
     CDS             11916..12395
                     /locus_tag="VFF28_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44224.1"
                     /translation="MLSRIVNNKKGSDGGIGDDVTERSVLYMVFSFCLCGIMIYFLLQ
                     MYYYIGDAVNIDPQIGKFAAGQQLASCVASDGIPGSSIDMRKFTQEGLNLCYSSNPEA
                     KIAYILELKIEDEQRKIHTDNWVEEKPFSNSKPATVIVKKEDESIEGVLSIGEQIVS"
BASE COUNT         3907 a         2296 c         2682 g         3517 t
ORIGIN      
        1 attattagga gtgcatgaaa tacaggagcc ccctgagcag ccttgagtac agcttgccaa
       61 tttatcttca tcagtgtaag gatttgcata acaacttcca gctgagcatc cggtatcata
      121 gcatgtccgt tggtgataca cgccattctc cttgcagtag tttgatcccc agggcgtgca
      181 gaatttttgg caatcttctt ttttatcctc cagtttgccg caggaatcat accaataagt
      241 attatcatca aaacaattac tggtgtgatg tgaaatgcag cattgagcct gagtattgga
      301 atactttttc tcaagattat actcagtaac ctgataattg ttgctttgtg caaaaaagtt
      361 gttatcgtca atatcgccgc agttccaatt atcgtcaagg gcatcaccca aatcattgtt
      421 atctccgccg tacaccccca tcacgataaa tggccgcgtt gtttcccctg cacaattata
      481 cccgcattta ttttttcccc aaagtgcgca tgattcatag cttggcgcac aatagacagt
      541 ttgagttcct gcttcgtcac atatctcgtt attaaaacta tcccatgagc ccgcatcact
      601 gccggaattg tcagggcttg catcccttcc ataatatgta atgtagcgat atttccaata
      661 ggaattgtca accttgccga tatagccata atctgtattc tgggtgcttc caggaatggt
      721 ccttagccat gttcctgatt tttttgaccc cacaaaaccg atgcctgcat atgcatcgat
      781 atcctctccc ttgcagtcca tatcataatc ctcactctta taatcatcat cagtgtaaag
      841 gtcataacac tctgaagagt ctgagctgtc agtagattta ctggatatat aatcaagtct
      901 atagcatttg ccgctttgca gagtaaaccc tggaccctca tcctcataat cataagttct
      961 atcattccat tcaggcattc tatttgagta atcgttaaag actatagatc cagagtcaga
     1021 gcaggccatt attgagcata ttcttgtgca gtctggacct atgcagctaa caccttcatc
     1081 taaataccct atagggcata gcggagggca ttcatttgaa gctgcggcaa aatcactatg
     1141 ggtgggctgc tcaggatatt ttggaaaaat aaatgtttta tcgtctgttt cctcagacac
     1201 tgcaacagat atagagcaaa ttaagaaaat gaggaataag gctatgcatt tattaaaaag
     1261 catttacacc cccctaatgg taaagaataa ttgaaatcta tataaatttt cctaactttt
     1321 gtaggtatta agctctagtc gtttgttcaa ttgttaaatc tatattctag tgcttggcaa
     1381 agactttaaa ggggtgcttg gctgtgatgg gtggtcaacc tactttgtat atgctaagga
     1441 tactggaatt ttgcttcagc gctgctgggc tcatgctctt agggagttaa aggagatctg
     1501 ctataataaa aaaaggcagg acaaaagcct tgtgaaagcg ctgcatgata tatttgagaa
     1561 agtaaaggaa gcaagaacaa gcagagaaat aagagagcaa ttctacatgg aattagtggg
     1621 tgttttaatg atggagttac ctcccacaag tttataacag gcctatatag gattgtattg
     1681 atttaaaacc atctgtttgc ctgattcaat ttttcctatt ccttatcatc tctataaata
     1741 caagaagaac aataacaaac acggctccaa taagaaacca aagtgcgatg ctggtatctg
     1801 aggttgtcac atttaccaca gcctcaggcg ctttttcgat agcagccctt gcacttttct
     1861 ctactatgct gggcgtaata gcctgcactg cctcatccct tacagattcc acaaatggag
     1921 cagaggctgc tccctttgcg ctctgcatca aaggggcagc agcaagtatc tttgcagaag
     1981 cagaataata tgtagaatac caatgaagga gccctgctcc agcagcagca atcaaacccg
     2041 caggaagaat agaccttaat ttttccttta tcccatgtat agacttaggt gctattatta
     2101 tgtacttgtt tgcaagctgg taatgattga cctcttttcc cttcgcacta taatgatatt
     2161 cctcagacac aactattccc gacttgataa gcaactgaag gttgtaatgc acagtagata
     2221 taggcagctt aagttcatca gccatgactg actcggtaga atcctctttc tcagaaagat
     2281 agtccagtat cttcctgcag ctgtcattgg ttattacctg agctagctct tttgccttgc
     2341 tttccttcag gcttacaagc atgaactttt ctttgcccat atactttcac ttgacataat
     2401 aaaccaggtt tatattcgca ttgacagtga catcctttgg cattaccttt gcagcctcag
     2461 ccacagcagc actgccgtcg cctgctctca tagcataaaa gtatggctgc gcataatagt
     2521 ttgcctctga cactgtcttt aattttccaa gagagactcc aaggcttctt gctattgtct
     2581 cagccttgct ctttgcatcc tgtgtcgctt ttgttattgc ctgctcccta tactcagctt
     2641 cttttgcatc gctaagctgg aaattgatgt tctgtatctg gtttgctcca ttatccacag
     2701 cagcatcgac aatatctcct actttggata ggtcagttgt ttttactttt aaagtttgtg
     2761 ttgctctcca tccttcaagc tttgaaccct cattttctgt ccatgtcctc tcttcgtaaa
     2821 ggtttaggct ctcggtctgg atgtccttct catttattcc ttttgcctta aggccatcca
     2881 ctatgtcatt gatgaccttg tttgcctcgt tttgcgcttc atcagccgta tctttgacga
     2941 tagatatccc tgcccatacc tctgcctcat caggctttgc taaaagctct gactgtcctg
     3001 acacattaat tgtattgcca tcttttgttt ggcatcctgt aagggcaagt atcgcaagca
     3061 ccaaaactgc cccgatagta tacttgattt tcatttggtt cacctcacca ggcacttata
     3121 gttacaaggc atttatatat aatttactat aggttttatc ccagttgaag ctatttttgg
     3181 caagtccttc tcttggtata agccctacgg cctcaccttg acgatatccc ctctctatcc
     3241 tttcctctcc ttttgacaca tatatcccgc cagggtatag ggtatttatg tctttgaaca
     3301 ctgcacgaaa gcggagaaag acacaaaaca aaggtatgtt tggagactga aagctagggt
     3361 ttatatacat taagatgaat tattgatgca tgaatatggt agagctaact tcttcccata
     3421 agcatgaact catcgacatc acctataagg taaaggccat cgtccaatca tcaaaagtca
     3481 aagaaggcat ctgtcaaatc ttcgtccccc atgcaactgc agcaatcact attaaacgag
     3541 aacgctgacc caaacataaa ggaagacatc cttaaagcga taaacaagat agtgcctaaa
     3601 acatgatgat tatctccatg acggcataga caacaacgag agcagcccat atcaagtcga
     3661 cgttgataga cctctatacc cataagtggt ggagagttgc agcttggcac ctggcaggac
     3721 atcttcttct gtgagtttga tggccggaag aaaaaggaaa gttatcataa aaattagcga
     3781 gtgatacatt tgtaccattt ttttggatac aaatgtaacc gatacattta tatacatttg
     3841 taccaattct ttgtttacat ttgtaactaa tgaatatctg caagcacttg gaaagactaa
     3901 aggaaagctt ggaagttata gatgaaagta taacccgggg cattataaga aggcaaagaa
     3961 caataggatt tagtttatct gcagcatctg ctgatatgtt tgagattttc ctgcacaaag
     4021 agaaactggt tgaccccgga tttgttgtaa agcatgaatg gtttaagtca aaaaacaagc
     4081 ttgaggaaaa atttcctttc gactttgcag ccaaggctgt aatttttgag ctcatgggat
     4141 tgatggaaga aaaaagggat gctctctgtt atggtgtgcc taaggaagag aaatatattc
     4201 gggatgcaat agataatttt aatgagctaa aaaaaatatt tgacacaaag gggattattc
     4261 atgagtaagt tcctaagcta tgctgttgat tttgcctcgt ttcttttgga gaaggttgac
     4321 agcgccaaaa ttagcagcat catcctcttt ggctctgttg caaggaatga agcaagcctt
     4381 gaaagcgata ttgatatttt cattgaggta gctggcagct ttgacttaaa aaagcagata
     4441 gagaatgccc ggctggattt ttttgcttcg gcaaggttca atgattattg gaggctgaag
     4501 gggataaata atgatataaa tgtgcttgct ggaaaagtag aggaatggaa ggacataaaa
     4561 aacagtataa tctccaatgg tgttgtttta tacggtaaat atgaatccgt cccaagaggc
     4621 gctgtccata agactatttt ttcatgggag aagattaagc ctgaatcaag gagggtgctg
     4681 ctccataaga agttgtttgg atataaggct ggggaaaaaa gataccctgg ccttgcagaa
     4741 aaatatgaag gtgaaagggt gggtaaagga agtattatgg tgcctgcaag gcatacaaac
     4801 gttttcatgg aagtttttag agagatgaag attgcagtaa agatcaggaa gatagtagag
     4861 tatagttgag gagaaatatg caaaaaaagg gagtgacaga tgttcatttt aattggatct
     4921 ttatccttat tgcaggcgcg gtcatattca tgttctttat tacgattgtc aacaagcaaa
     4981 gggcattctc tgaagtcaag gtggcccaaa gccttgtagt aagccttgat gctatcctta
     5041 caggagcaga ggtctcgaca aggacagtaa atataatcaa ggaccttcca aaagcagaca
     5101 ttggttttga gtgcaacaac tactttgtcg gaggcatccc aagggatacc ggaggcagtg
     5161 taatatttgc tccctctgtc ttgaaaggaa gaaggattat tgcctgggct gtagactgga
     5221 agatgccctt taaggtggca aacttcttgt acctcactca ccctgaagtc aggtatataa
     5281 tagtaaacga tacaagcagc tcggcaaatc tggctcaaaa gctgtttgat gagctcccca
     5341 aagagttgaa caaggaaatc gtggcaaagg ataagattgg ggatataatc gataagaaca
     5401 actacaaagt gaagtttatt ttcttcaatg acaatatcca acctccaggt gttcctaaac
     5461 ttaaagatat gccctttaag gatgttactg caatatcact gcctgcctcg gggattatcg
     5521 cttcgtcagg ggagctttcg ttttgggaaa agaacaaagt aggagctttc gaatggattg
     5581 acaagggcga cagcttctac atagaaaaag aaagcctcta tggcgcaata tttgccgaaa
     5641 acaaagatac atatgactgc gtgatgagaa aggcctttga caagctttct tatgtcagca
     5701 gggtctaccg cagtcgaagt tcagagcttg gcacatttta tcgtgcatat ccccctaaac
     5761 aatattgtgc aaattaccat gtaacaactg agtttgacaa tacccttgtt cctctttcaa
     5821 cacagctagc tcaatcattc ccgacaacta tgccagagat aacctcgctc aaggaagctg
     5881 ctgacaatat agagcagcag aaccaaaaag cctcgctaaa gacctgcgcc ctggtgtact
     5941 aagatgaaaa agtctcagct tcagatgggt gaatcggtcg ctgtcttgtt tatctttttc
     6001 atacttatag ccttcggctt ctctttctat atgaatataa tgaaaggttc acagaagata
     6061 gacctaaagg aaaatgccga gctaaaggcg ataagcatag cccagagggc ttcattcttc
     6121 ccagagcttg aatgctctga agaaatagta aaagaaggct gtgtcgatgt atataagctt
     6181 gacgcagcag cagacataat agatgacagc aatgtggttt actttgagat gtttggctac
     6241 agtttgataa gggctgtcca gcttttccct gaagaagaga aagtctgggt catatataat
     6301 aataccctgc caaactatga aagcagactt acctcattta tccctgtatc tatatataat
     6361 gccaccatga aaaggtatac ctttggcatg cttgctgtcg atgtattctc aggggtcgtt
     6421 gaagaatgaa aaggtcccag atggagataa tggggcttgc aataatagtc atattgatct
     6481 ctattggtat gctctttgcc ataaagtttg ttgttttcaa aaagccagag ccattcaagt
     6541 cagaatatac aaagacagag ctagcctcaa acatcttgac aagccttatg cggacgacca
     6601 tggaaggctg cagcgatctt agcatggaag acatctatca agcctgcgga aaagaccctg
     6661 acaatccgcc ggttgtatgc tctcctgctg aggacgcctg ccagtttcta aacaggacaa
     6721 caaggcgtat cctaaaccta acattggtgg aatggaacaa aggctatgag ctccatgcct
     6781 ctacagtcac agattcgata atagatgtcg gcagatgccc tggagcaaag aagcaaaagc
     6841 cgtatactat tcctatcgat gcttcaagca agaactcttt gaagataacc ctggatgttt
     6901 gcgataaatg agttacccct acaaatggta taatgtagga ttttggtggg ggtaaacagc
     6961 aaagtttaaa taatacttgc cccgagaata aaccttatca taaaacgagg tgcattatcg
     7021 atgaaaaaag gccaaagcct tagcttgaat acgattataa tagcagctat cgttcttatt
     7081 gtccttattg tcttatgggc gatatttaca ggaaggatgg gtaagtttac cactgattta
     7141 gcaaaagaag gcgaagtaaa aacttgcgaa aagatggggg gcgtaggccc tcaggagccg
     7201 ccttgtgata caaataaaaa accggtaatc tcatcagata ctacaggaac aaccgtgtgt
     7261 tgcgtaccat gaaaaaagct caaagcctaa gccttaatac gataataata gcagcaatcg
     7321 tgcttatagt actaatagtc ttatgggcga tatttacagg aaggatggga aagttcacct
     7381 caggtgtaaa tgagcaggat ttgcagtccc aagcagcagc tgcaaatgta ggaaatactg
     7441 aatgtgacgg aaaatgtttt accactgttg gaactaaaga tggaaagtgg gagctaatga
     7501 aagttaaggg agaaaaagca tctgcctgca gtaccagtca aaaatcagta ggaactgcct
     7561 tttctgattt agagaaaggt caagtgtgct gcatttcaaa gacagtacta tcaagcaatc
     7621 ctgcctgcag ttaggatgct caaatccctc aacggcgaca ccatcctaaa actttctttt
     7681 ctttttattc ttcttatcct tttaggctat cttatataca aactttttat catcgatgag
     7741 acaataaacc tcatgataaa caacaggact tttggttgac atgtcaaaaa cctccaggtt
     7801 agttttaatc cgcttaattt agcggtttct gagcatgcta aaaaatcctc gataacacgc
     7861 tcctattgta gggggttttt gacaaaaggt ttatataata cgctcttggt aaaagagatg
     7921 atggttctaa ggaaaaaagc tcaagccctg tttgtctgga taaaaatatt tctgtgcatc
     7981 gttatagtca tagccgcctt agcagttgta tggagtattt taaatgtcag accgtaaggg
     8041 aaagatgatg gaaataatgc ttggaggtat cgtaatagtg gttacgttgg ttgttcttat
     8101 ccaagtattg cttgtcttaa ttggcgcagg aaaggcaaag ggcgcagagc agctatgcga
     8161 aaacttcaac caagcaaggc tcgctggcca ggttgaaata aaggattatc ctgtcttcaa
     8221 cttggtgaaa agggccccct gtaagaagca gagcctaaag cttccgctca aggacagtga
     8281 tctagaagga acaatggatg atatcggttt acacatccag aaatgctgga agcaatggct
     8341 cgaaggaagg actgatcgga tttctgagct gttgagcagt tataaagaat gtgtaacctg
     8401 ctactctttt gaaatttcta agggtattcc gacatttcaa ggctctgacc taaaaaaata
     8461 tctgagtgat aatgtacaga tagtaagaga tacaagcgat agatgcaata taaatggagg
     8521 aggatattgc atagaagagg aaagttgtac tgccggcggc ggaacaaaag taccttcaag
     8581 ctcttgctca actggtgaaa tctgctgcaa taaggcaaat gaatgtgaaa ataaaggggg
     8641 ctattgtagg gcaacatgtg atactagtga tgcagaatat gctggctgga attgcaagga
     8701 aggcagatgc tgtgtagaag gacagaacat gcaaagttat gaaggggtgt taaatagcaa
     8761 tcaaggcagg attttggttg ctgttgacaa ttttatccca gtcagcgaat catattctat
     8821 aactttttca acagtaaatg gtccttggtc tgattttaag gaacggctgc ctaattttgg
     8881 gtttcctgag gaaaaagcta attcgataat aatctccaag gcaaaagata cagaggggct
     8941 ttgtgatgaa taaaaaagca atcctgttat tttgggaaaa actggtatgg tgggcagcca
     9001 tgatcttgat tacattcatt gttttagctt tgatatttaa aagtaagggc gctatgacag
     9061 acatcatagg gtatatcttc agatgaaaaa agcatacacc tttatagaga ttattgtatt
     9121 tatggcagtt atagctgtcg cagtatttat agcccttgcg gtattttcac cgagatttca
     9181 aaaaattggg aaaatcatac tgggctatga actgcctgga aagccagatg aaggtttttc
     9241 aggaaaagaa gaagtaactg aaaaggttca ggcagctttc gatggttttt acggtgcttt
     9301 ggtaaaggca aagaatacaa acgagtgtta tatgaaatac cctagcctaa atctagggac
     9361 aaacgttcta gaggtagaat atatagagcg tatgaaaggc actaacctga ggcttatgaa
     9421 tgaaaaaggt ataaggattg cagaatattt tgtggatggc ttacagccat gcatagcatt
     9481 acccgacaag aaaagacaaa ttgaattaga acaatcaata agtaatataa acattaaagg
     9541 taacaataaa gccatctttt ggaacgatga gtattcgcta tttccaaaat acccttttct
     9601 gcatataatt gataaaaagt tttgtttttt tacagatagc tcaattatta gtccgcaagg
     9661 cgatgtctat ggagaaagaa caatcacaag agcatttacc gcaggattta atgaaaaaga
     9721 tatgacaaaa ataataaagc ctatctgtgg aggataagat gaacaaaaaa gccctggaga
     9781 tatccatctc tattttggtt attgcactga ttgcattagc tagtgcagta atagcccttg
     9841 caatttataa agtgactatt ggcaaagaag caaaagtaac agatagtctc atagcagata
     9901 aagaccaaga taagataatc gatatgtcgg acaaatgccc taataaaccg ggcattatag
     9961 agtatgccgg atgcccatca aaagataagt taacagagtg tgaaaatagc aaagagccca
    10021 atgcagaaaa ttgttaaaaa aagaagggga ttcacatggg gtcttgaagg aactgcaaga
    10081 ttgatttatg tagccctagg tttgattttt ttgcttgctc ttctaaaagg cgcttttaag
    10141 tatactggcg atgccgaaaa aagctttgat gatttggcaa gcctcttaaa cagccttgaa
    10201 gacaaggaca caaaacctct gcaactaacc atagacaaat caagctattt tttgtttttc
    10261 aataaaggct ttgacttaag atatgcatta actgggtatg gagggatggg ggtaacttta
    10321 aaagacagca ttactataaa gcggccccag caatgccctg ccggtactgc ttgccttgtc
    10381 tggtgtggca agttgacaga cgaggcaggc gatataaaga cctgcagcaa ggtaaaaaag
    10441 caagtcccga taaataaaga taaatccaag gccatcaaat tctaaggccg aaaaacatac
    10501 aatttcctac agggattttt cataaattat gagtacttat ccgagcagca tcccaatgaa
    10561 ggtgatacat ctaagggata tacactctat ttccaggacc acaaaggcac aataggtatg
    10621 tgtgataaaa agccttgttt tagtgatgaa gagattaata aaaggataag tttggctcag
    10681 gaagagatag ttaaaaaaaa ttttgagttt gccatcaaat cattgattgg agttgatgcc
    10741 aacagcgacg ggaatataga aacgttcttc tatatcaacg acctggttaa agatgatttt
    10801 ggtagtaatt actataagta caaatttggc tataaaactg aagatccaag ccaggcagcc
    10861 ttccttgaga cagtgcagga gaaagagaaa aaagagtttg aattaaagat ccgttcctat
    10921 gccgctgctg gcaaaattgt aaatatagat gaggggaatt cgggttcgat tgctaagttt
    10981 tcctcagaca gggagctgaa agtttaagat gccaaagaag gggatggcaa taagggagtt
    11041 gtctaatttt attattggtg cctttgtgtt tattacactc atagggatag gggttagcta
    11101 tgggaaggga gaggccgtct ttaaggtgca gttggcaaaa gatattggcc ttatgattga
    11161 gcagatgtac aatacacctt caagtatgaa tatttatgag acctaccctg aggacgtctc
    11221 atccttcaat attaaggtca aaggtaaaac cataacggtt ttttcaaagg aaggagactc
    11281 tacgaaggct gaatataatt ttaccctaaa cccaagttat cctgaacttg atattctctt
    11341 aaaaaaaccg caaaggatgt acattggcaa tattgcaggc aaaataacaa taaccgataa
    11401 agcccctgat ttaagaaagc tgaagtgcag caatgcagag atagaagtaa aaaatatatt
    11461 gatagattct ggattagaaa tatcaggtca aaaggataaa tcgcaagcag caaattctgt
    11521 tgcatattcg gttgtatcga ggctggaagg aaattatgat attgagcatg caaggaatga
    11581 ctatattgat gagatggttg gtactgcctt gccattcata ccctcaaaat ttgaggcaaa
    11641 ggtaaaagat acagacctga ttattggatt tagggcaaat gaaaaagagg atttggtggt
    11701 aagttactgg ccagattcaa aaaagagcaa tgaggctgaa tctcttgctt gcaggatagt
    11761 taatggtttt attgaaaaag ggcttatcaa agaaggatat ggtgtttctg ttgccatggc
    11821 tcaaacagat attttaagga atgataaggt atcgattctg gtagaatttg gggataagat
    11881 tgtaaagcct gtggatatgg gagaggttat atttgatgct atcaaggata gttaataaca
    11941 aaaagggaag tgacggagga attggcgatg acgtgacaga gagaagtgtg ctctatatgg
    12001 tttttagctt ttgcctttgc ggcatcatga tttatttcct attgcagatg tattattata
    12061 taggcgatgc agtaaatatt gatccacaaa ttggcaaatt tgccgcaggt caacaattgg
    12121 catcctgtgt tgcatccgat ggcatccccg gatcatccat tgacatgagg aaatttaccc
    12181 aagagggcct taatctctgt tatagctcaa atccagaagc aaaaatagca tatatcctgg
    12241 agttaaaaat agaagatgag caaagaaaga tacatacgga taactgggtg gaagaaaagc
    12301 ctttttccaa tagtaaacca gctacggtta tagtaaagaa ggaagatgaa tcgattgaag
    12361 gggtattgag tatcggtgag cagattgttt catagaaagg ca
//
LOCUS       DASRUK010000022         9136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1029892, whole genome shotgun sequence.
ACCESSION   DASRUK010000022 DASRUK010000000
VERSION     DASRUK010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 9136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9136
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029892"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..812
                     /locus_tag="VFF28_00930"
     CDS             198..812
                     /locus_tag="VFF28_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HZX44225.1"
                     /translation="MKNILVVGAHPDDIELGCGGTIARHLEQNNRVYALVLTNGEQGA
                     HAPQMVECLASLKSLGIADSDILFANYRDGHINDDHDTVDYIEKMIRKLGIDVVYAND
                     PNDNHQDHRNTSRAVSSAARKVNSILLFQGPSTTVSFQPHYFIGLKERHIKKKIESLA
                     NYRTQIAKGCFSLQWIESLAKVNGARCGAPYAESFAINHIFEGH"
     gene            813..1568
                     /locus_tag="VFF28_00935"
     CDS             813..1568
                     /locus_tag="VFF28_00935"
                     /inference="COORDINATES: protein motif:HMM:NF020463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HZX44226.1"
                     /translation="MIAALHQPNFLPYLGFFDKMKKADLFVIRDEVLFVRKEYHQRNK
                     IRINSNDNLNHPQSKWLKVPVFEQEDYIMNIGIDNRFLAGGKPWRENMLHEVKASYHG
                     SIYFSEYFQGFSDIISGVDAGLLSLNMKLIDFLRTKFDIDTKIVLASKLGLKDAHYQK
                     TDASLDIIDICKEVGAKTYLSGIGGKDYLDLKRFEEEGIKVDFQEYVHPTYKQRYPGF
                     VSYMCALDALFNVGRFPEVRDDNNNRRDPAGKA"
     gene            1528..3042
                     /locus_tag="VFF28_00940"
     CDS             1528..3042
                     /locus_tag="VFF28_00940"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZX44227.1"
                     /translation="MTITIGVIQRGKLEKLMPDLLKLEKNWVSIGERPWSSENFKVDL
                     PGKWKLSLYASYQKRIVGYAICSIEGNIGRLNKILVDESLRNKGIGSKLWNELLRRCR
                     ELGLQKMEFKSSPDNPSAISFYRKKGCLFYGEIMGFDGKVRNTNMYVFRTKSRISHSK
                     PSLNALDEDAVVNALQNKQLGSGEIAERFVAALCRYQQRRDGIAVNSGTSGLFLALRA
                     LGITAGDEVIIPSYVCYSVLSAVLQAGAKPVIADINSDDYCLSYSSAMSKATKNTKSI
                     IVPHMFGRAAKDIDKFVSSSCPVIEDCAQSLGAKQDGKKAGTLGLISVYSFYATKVIS
                     TGIGGAVLSDDESINLSLKDMIRSDKRENLAETYNLRMSDLSASLGLSQLKRADDMIE
                     RRIEIAKKYLALFKESDVDFNLPSLDGIFYRFIIRHALKDFFMKSVNARGLACCEPVY
                     KPLHVYLGLPEQEFPNTMRAFREAVSIPIYPSLTEEQVEECAGIILNWRQEVKK"
     gene            3039..5087
                     /locus_tag="VFF28_00945"
     CDS             3039..5087
                     /locus_tag="VFF28_00945"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZX44228.1"
                     /translation="MKMLVLLLILLSIPSVLGDQLEIFSTRSGFISPVYRDVSFARLM
                     DAGLTSAGLMYFDKNGTYFPNSAVHYEWFDMSNIPGGSVIEDAVLYKYHDSSPIRGDI
                     NYKMVPAMIVGVNRVTLAKLTQGFTEDITYPSGYRTMENLGLVSTAATGTALVFNVTS
                     EIQKYIDGEPNYGFAFLGNYNTGGFSSSRYYFRNSANKPKLLVKYKLKPVWVPACGDG
                     KLEGQEECDDGNLIGGDGCSIYCSKESTPGNDTDEICQFAYSAAATSQNNLGSLASFA
                     EFEPDAPKKDCNVWSGYGYSWTPQNWNIKANLTLKYKIPVKAESVTIYGDYAVCWDRV
                     WAKDSATGETQQIFTGPNNTCELTIPANISFAADTLILETCGWSWSATDAVKLCGFGA
                     KEPDRKVAICDWKYCRKGAASISVDDSFTSCAPELDAKGYKGTYFLSHTDTLTDLQWD
                     ELAALYKNGHEIGTHSRSHLCTGLDDSMYKEELEDNIQDITQNLGISRENIKTHSYPC
                     GMFSAEKEKILKELGYSAARGYNINQLERPLPADLFNLKSYNTVGYPGGEYDPPSYIA
                     AVDEAEKSGRWFSLVMHSKCSDSGVIEYLPKKDLWVDNIGNVASYIDQKQDCKITDYR
                     ETSRDIKFRTEGCGGVFMKHLIKGPGAVWMDGSPVIFYSRDGYIFFEGKDNAEVRITR
                     "
     gene            5084..5929
                     /locus_tag="VFF28_00950"
     CDS             5084..5929
                     /locus_tag="VFF28_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44229.1"
                     /translation="MTKLFYFIPLLLLLIGLALFLVPMQRGLPKGNGIVTNVWADSKR
                     IETARQNGLKYLFVDVGDITKDGALVTPKEEIEEFLSLAKNKGFVLLPYTEVNTENYD
                     FSSSRFMANLIGAHKELVDMGFDGAYLDVEAVQKDKEADYLRLVRLLRKELPSGSIIA
                     AYAGFLKDSDNRWEWDHDFYQDVSESVDLIFVAGFDLDAKDASEYDEYIVSQMEDISS
                     SGYKSYFMLGVPTHKKYPETIDSAMAAYRKALEGLPNDKFLGVAIFSDWTIDDGEWET
                     YRRYR"
     gene            5926..7122
                     /locus_tag="VFF28_00955"
     CDS             5926..7122
                     /locus_tag="VFF28_00955"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZX44230.1"
                     /translation="MKGPFSIWPFFGLTIWIDTFTHILRWNDESKIDPYKPIRSVSVM
                     IPAHREQKDIVSSLKSIYRQRYPVKNVFVVGDKFSDATRSIIGSLSKRYGNLKYIESP
                     FASKAMKVNYLVKNFNEKLGDFIYVMDARVVIEEESIERIISCFNKRKVAAVTSYGRV
                     SIPKNFLSRSYHYGKAWVNEIGRFRKNAQEKRKAVFVICGASTIYRKNVLKRNPIPSC
                     TKTEDTHYTWVLQRKGYKIRVADDAVASSPDIDGYGLQGITNQIKQSYRWSAGTMQCL
                     VYESGNIMKNKKLFFSTIIPGFVESVTYSIVLVLSPFFLFFTPELVAGFFIGDTVFSI
                     FGTLVITPSKFKKTVIHYPQILLFKYINALIFVIAFFVVNFGAIFGNPNNWSNEWTPL
                     ETTVEG"
     gene            complement(7345..8064)
                     /locus_tag="VFF28_00960"
     CDS             complement(7345..8064)
                     /locus_tag="VFF28_00960"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HZX44231.1"
                     /translation="MIKNKILVLSGKGGVGKTTVAVNFALLLALLGKRTGILDADMHG
                     PNVPKMLGLDDMKLANHGDKMIPIQHESGLKVISLAFIINEKDSVIWRGPLKHNAIKQ
                     FLEDVEWGELDYLVIDFPPGTGDEQISTAQLVGKATGAVIVSTPQKVAILDSVRSIDF
                     CRKMGIPLLGIIENMSGGIFGKGTISKVAEENNLPFLGSLELDEKIARSGDEGKPFAA
                     GDSVLSLSFKNIVSRIVEKIS"
     gene            complement(8070..8654)
                     /gene="epsC"
                     /locus_tag="VFF28_00965"
     CDS             complement(8070..8654)
                     /gene="epsC"
                     /locus_tag="VFF28_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HZX44232.1"
                     /translation="MFDTLVTAYKKDPALRGINALEVVLYQGVHAILLHRIAHLFYSL
                     KLPFIPRLISQIGRLLTNIEIHPGAKIGKRFFIDHGAGIVIGETTEIGNDVMMYHGVT
                     LGGHGWWVDAKGAKRHPTIEDNVVLGVGCKVLGAVTVGKNSKIGADAVVIHDVPANST
                     VVGELGKFVIKSGAKVRREEIQKVEVPETEWYQI"
     gene            complement(8647..>9136)
                     /locus_tag="VFF28_00970"
     CDS             complement(8647..>9136)
                     /locus_tag="VFF28_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014662501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZX44233.1"
                     /translation="PANPEIHRKTTAVEIIEQVPDLDAFVAGIGTGGTITGVGEVLRK
                     KAGKTVQIIAVEPDASPVLSGGAPGPHEIQGIGAGFVPDVLNTKVYDEVIRVTNDNAF
                     ETSRRLAREEGIFVGISSGAAAWAALQIAKKLGKGKKVVVILPDHGERYLSTALFGDD
                     NV"
BASE COUNT         2663 a         1929 c         2057 g         2487 t
ORIGIN      
        1 aataccccgc cccttggggc ggttgggatt ttattttata atttttcaca ttagtatcgt
       61 ctgaaaaagt tatggatgca agcgattaac tactctcgaa gtaaaagaaa atattataaa
      121 taaataacct atatgacaag ataacgattg atatattatc ataaagactt tattcctaaa
      181 tagtttatga gggcaaaatg aagaatatac ttgtggtcgg cgcacaccca gatgacatag
      241 agcttggatg cggcggtact atagcaaggc atctggagca gaataacagg gtgtatgctc
      301 ttgtcttgac aaatggtgaa caaggcgcac atgcgccaca gatggttgaa tgccttgcat
      361 cccttaagtc ccttggcatc gctgactcag atatactctt tgccaactac cgggatggcc
      421 acatcaatga cgaccatgat actgttgatt acattgaaaa gatgataagg aagctcggga
      481 tagacgttgt gtatgcaaac gacccaaacg acaaccatca ggaccatagg aacacgagca
      541 gggcagtctc ttctgccgca cgaaaggtca actctatact tctatttcaa ggtccatcaa
      601 ccactgtctc tttccagccg cactatttta tcggcctcaa agaaaggcat atcaaaaaaa
      661 agatagagtc cttggcaaat tacaggacac agatagccaa aggatgtttt tcactgcaat
      721 ggatagagag cctggccaag gtcaacggtg caagatgcgg cgcaccttat gcagaatcgt
      781 ttgcgataaa tcatatcttt gagggtcatt agatgatagc tgccctgcat cagccgaatt
      841 ttttgccata tttgggtttt tttgacaaga tgaagaaggc tgatcttttt gtgataaggg
      901 acgaggtgct ttttgtgagg aaggagtatc atcagcgaaa caagataagg ataaacagca
      961 acgacaactt gaaccatcct cagtcaaaat ggctaaaggt acccgtattc gaacaggagg
     1021 attatatcat gaatatcggc atcgacaata gattcttggc cggcggtaag ccctggcggg
     1081 aaaatatgct gcatgaggta aaggcaagct atcatgggtc gatatacttc tcagaatatt
     1141 ttcagggatt tagcgatatc atcagcggcg ttgatgccgg cctcctctca ttgaacatga
     1201 agcttattga tttcttaagg actaaatttg atatagatac aaagatcgta ctggcaagca
     1261 agctcggcct aaaagacgca cattatcaaa agactgatgc ttcgcttgat atcatagaca
     1321 tatgcaaaga ggtaggggca aaaacctacc tctcagggat tggcggaaaa gattaccttg
     1381 atcttaagcg atttgaagaa gagggcataa aggtcgactt ccaggaatat gtacatccta
     1441 catacaagca aaggtatcct ggatttgtct catatatgtg cgctcttgac gcattgttca
     1501 atgttggcag atttccggag gttcgtgatg acaataacaa taggcgtgat ccagcgggga
     1561 aagcttgaaa agctgatgcc tgatttgctg aagcttgaaa aaaattgggt ttccataggt
     1621 gaaaggccat ggagctcaga gaacttcaag gtagacctgc ctggtaagtg gaagcttagt
     1681 ttgtatgcat cctatcaaaa aaggatagta ggctatgcga tatgctccat cgaagggaat
     1741 ataggcaggc tcaataagat ccttgtcgat gaaagcttaa gaaacaaggg tataggttca
     1801 aagctttgga acgagctgct gagacgatgc agggagcttg gccttcagaa gatggagttt
     1861 aaatcaagcc ctgacaatcc atctgccatc tctttctaca ggaaaaaagg gtgcctgttt
     1921 tatggagaga tcatgggctt tgatggcaag gtcaggaaca caaacatgta tgtgttcagg
     1981 actaagtcaa ggataagcca ttctaaacct tcacttaatg cgcttgatga agatgctgtt
     2041 gtaaatgcac tgcagaacaa gcagctgggc agcggagaga tagcagagag atttgtcgct
     2101 gcactgtgca ggtaccagca aagaagggat ggaatagccg taaacagcgg aacttctggc
     2161 ctgtttcttg ctcttcgagc tcttggaata acagcagggg atgaagtgat aatcccttct
     2221 tatgtctgtt actctgttct gtctgctgtc ctgcaggcag gagcaaaacc tgtcatcgca
     2281 gatataaaca gcgacgacta ctgcctgtct tatagcagcg caatgtcaaa ggccacaaaa
     2341 aataccaaat ctatcatcgt gcctcatatg tttggaagag cagcaaaaga tatcgataag
     2401 tttgtctcct caagctgtcc tgtgatcgag gattgcgcac aatcccttgg agcaaagcag
     2461 gatggaaaaa aagcaggcac cttgggcctg atatcagtat attcattcta tgcgacaaag
     2521 gttatctcca ctggcattgg aggggctgtg ttgtccgatg atgagtccat caatctgtcc
     2581 ctgaaagaca tgataaggtc agacaagagg gagaaccttg cagagaccta caacctcagg
     2641 atgtctgacc tttcagcgtc gcttggccta agtcagctaa agagggctga tgacatgata
     2701 gagcgcagga tagagatagc aaaaaaatat cttgctctgt ttaaagaaag cgatgttgat
     2761 ttcaacctgc cttcacttga tggcattttc tacaggttca taataaggca tgcgcttaag
     2821 gattttttca tgaaaagtgt aaatgccagg ggcctcgcct gctgtgagcc ggtctacaag
     2881 cccctgcacg tatatctcgg tcttccagag caggaatttc caaacactat gagggctttt
     2941 cgggaagcgg tgtctatccc aatctaccct agccttacag aggagcaggt tgaagagtgt
     3001 gctgggataa tcctcaattg gaggcaggag gtaaaaaaat gaaaatgctg gtgctattgt
     3061 tgatattgct gagcattcca agcgtacttg gcgatcagtt ggagatattt tctacacggt
     3121 caggctttat atcgcctgta tatcgggatg taagctttgc aaggcttatg gatgcaggcc
     3181 ttacttcagc aggtcttatg tattttgaca aaaatggaac ttatttccca aacagcgctg
     3241 ttcattatga atggtttgat atgtcaaata tccctggtgg ctctgtgata gaggacgctg
     3301 tgctctacaa ataccatgac agctcaccta tcagaggtga tatcaactac aagatggttc
     3361 ctgccatgat tgttggagta aatagggtta ccctggcaaa acttacgcaa ggtttcaccg
     3421 aggatataac ttatccgtca ggatacagga ccatggaaaa tcttggtctt gtaagcactg
     3481 cggcaactgg gacagctctg gtgttcaatg tgacaagcga gatacagaag tacattgatg
     3541 gagagcctaa ttatggcttt gctttccttg gaaattataa cacaggagga tttagttcat
     3601 caaggtacta ttttagaaat agtgcaaata aaccaaaatt gctggtgaaa tataagctca
     3661 agccagtgtg ggttcctgca tgcggagatg gaaagcttga aggccaggag gagtgcgatg
     3721 atggaaattt gataggcggc gacggttgtt caatatattg tagtaaagaa agcacgcctg
     3781 gtaatgatac ggatgagatt tgccaatttg cctattctgc tgctgcaacc tcgcaaaata
     3841 accttggttc gcttgcttca tttgccgagt ttgagccaga tgctccaaaa aaggactgta
     3901 atgtttggag cggctatggc tatagctgga ctccgcagaa ctggaacata aaagcaaacc
     3961 ttaccctgaa atacaagata cctgtaaaag cagaatccgt aactatctat ggtgactatg
     4021 ctgtatgctg ggatagggtc tgggcaaaag acagcgccac aggtgagact cagcagattt
     4081 ttaccggccc aaacaacaca tgcgagctca ctattcctgc aaatatatca tttgctgcag
     4141 acacattgat acttgagaca tgcggatgga gctggtctgc cacagatgca gttaagcttt
     4201 gcggctttgg cgcaaaagaa ccagaccgca aggtggctat ctgcgactgg aaatattgcc
     4261 gtaagggcgc ggcgtctata tctgtcgacg atagttttac ctcatgtgcc ccagagcttg
     4321 atgcaaaggg ctacaaggga acatatttcc tgagccacac agacacttta acagatttgc
     4381 agtgggatga gcttgcagca ttgtacaaga atggccatga gatagggacg cattctagaa
     4441 gccacctatg tacaggcctt gacgacagca tgtacaaaga ggaattggaa gataatatac
     4501 aagacataac ccaaaatcta ggtatctcca gagagaacat taagacccat tcatatcctt
     4561 gcggtatgtt ctctgctgag aaagaaaaga tactaaagga gcttggatac tcagctgcaa
     4621 gagggtataa catcaaccag cttgaaaggc cattgcctgc tgatttattc aacttaaaaa
     4681 gctataacac tgtaggctat cctggaggag agtacgatcc gccaagttat attgcagctg
     4741 tagacgaggc tgaaaagagc ggcagatggt ttagcctggt catgcattcc aagtgcagcg
     4801 acagcggtgt gatagagtac cttccaaaaa aagatctgtg ggtagataat ataggaaacg
     4861 ttgccagcta tatcgaccag aaacaggatt gcaagataac agactatcgt gaaacaagca
     4921 gggatataaa attcaggaca gaaggctgcg gcggcgtttt catgaagcac ctgataaaag
     4981 gtcctggagc agtatggatg gatgggagtc cggtcatttt ctattcaaga gacggatata
     5041 tattctttga aggaaaagac aatgctgagg taaggataac aagatgacca aactttttta
     5101 ttttattccc cttttgcttc tgttgatagg ccttgcctta ttccttgtcc cgatgcaacg
     5161 cggccttcct aaaggaaatg gtatagtgac aaatgtttgg gcagattcaa aaaggataga
     5221 gactgcaagg caaaacggcc tgaaatactt atttgtcgat gtcggagata taactaaaga
     5281 tggagcgctt gtcacaccaa aagaggagat agaagagttt ctgtcactgg caaaaaacaa
     5341 gggttttgtg cttcttccct acaccgaagt caacactgaa aactacgatt tttccagcag
     5401 caggtttatg gctaacctca tcggcgccca taaagagctt gtagatatgg gatttgacgg
     5461 agcctatctt gatgtagaag ctgtccaaaa agataaggaa gcagactatc taaggcttgt
     5521 tagattgcta agaaaagagc ttccttcagg ctctataatt gcagcctatg caggctttct
     5581 gaaagactca gacaacaggt gggagtggga ccatgatttc taccaggatg tttctgagag
     5641 tgtggacttg atatttgtag caggctttga ccttgatgca aaggatgcct ctgaatatga
     5701 tgaatatatc gtcagccaga tggaagatat ctcttcaagc ggctataaga gttattttat
     5761 gcttggcgtc ccgacgcaca agaaataccc tgaaactatt gactcagcaa tggctgctta
     5821 cagaaaagcc cttgagggcc ttccaaatga caagttcctt ggtgtagcca tattttcaga
     5881 ctggactata gatgacggtg aatgggagac atatcggagg tacagatgaa aggacctttt
     5941 tcaatatggc ctttttttgg gcttactatc tggatagaca ctttcacgca tatcctgcgg
     6001 tggaacgatg aatcaaagat agacccatat aaaccaatcc ggtctgtctc tgtgatgatt
     6061 ccggcccaca gggagcaaaa agacatagta agcagcctaa agagcatcta tcgccaaaga
     6121 tatcctgtca agaacgtctt tgttgtagga gacaagttct cagatgctac aagatctata
     6181 atcgggtcgc tctctaagag gtacggcaat ctgaaatata tagaaagtcc gtttgcttca
     6241 aaggcgatga aagtaaatta ccttgtcaaa aatttcaatg aaaagcttgg agattttatc
     6301 tacgttatgg atgcaagagt tgtcatagaa gaagagtcga tagaaaggat aatctcatgt
     6361 tttaacaaaa gaaaggttgc agctgtaacc tcctatggaa gggtaagcat accaaaaaac
     6421 ttcctttccc gttcctacca ttatggtaag gcatgggtaa atgagatagg aagatttaga
     6481 aaaaatgccc aggaaaagag aaaggctgtc tttgtcatat gcggagcttc gactatctac
     6541 cgtaagaatg tgcttaagag gaatccgatc ccttcctgca caaagacaga ggatactcat
     6601 tatacctggg tacttcaaag gaaaggctat aagataaggg tggcagatga tgctgttgcc
     6661 tcttcgcctg atatcgacgg ctatggtctt caggggatca ccaatcagat aaaacagtcc
     6721 tacagatggt cagcagggac catgcaatgt ctggtctatg aaagcggcaa tatcatgaag
     6781 aataaaaagc tcttttttag cacgataatc ccaggatttg tcgaatccgt gacctacagc
     6841 atagtcctgg tcctttcacc attttttcta ttctttaccc cggagctcgt agctggtttt
     6901 ttcatcggag atactgtttt ctccatattt ggcactctcg taataacgcc tagcaagttt
     6961 aagaagacag ttatccatta tcctcagata ttgctgttca aatatatcaa tgcattgata
     7021 tttgtcatcg ctttctttgt tgtcaatttt ggagcgattt ttggaaaccc taacaattgg
     7081 tccaatgaat ggactccgct tgagacaaca gtagaaggat agttatctac ttttccttaa
     7141 attcttcgaa cttatcgtct gttacaataa tatagaacac aagtattcac ctcatttact
     7201 aatttatgga tttaaattct cactaaaaaa ttcagtcaga aattgccact atgtttgttt
     7261 acctctattt taccatagta atgggccttt gtttataaga ttatcgaaaa gtcctgatgt
     7321 tttaagattg atgcaccagc aaacttagga tatcttttca actattctac tgacgatatt
     7381 cttgaatgaa agagagagaa cagaatcacc tgcagcaaat ggcttgccct catcccctga
     7441 tctggcaatt ttttcatcaa gctccaggct tccaagaaaa gggaggttat tttcctctgc
     7501 caccttggat atggtgcctt tgccaaatat ccctcctgac atattttcaa ttattcccaa
     7561 aagggggatt cccatttttc tgcaaaagtc tatacttctt actgaatcaa ggattgccac
     7621 cttctgcggc gtagatacga tgacagcgcc tgtcgccttg cctacaagtt gtgctgtgct
     7681 tatctgctca tcgccggttc caggaggaaa gtctatcacc aggtaatcca gttcgcccca
     7741 ttctacatct tcaagaaact gctttattgc attgtgcttt aggggccccc tccagataac
     7801 cgaatccttt tcatttatta tgaaagccaa agaaatcact ttaaggccgg attcatgctg
     7861 tatagggatc atcttgtcac catggttggc aagcttcata tcatccagtc caagcatttt
     7921 gggcacattt ggcccatgca tgtctgcatc caatatccct gttctctttc ccaaaagcgc
     7981 aagcaaaaga gcaaaattta cagctacggt tgtttttccg actcctcctt ttccgctcag
     8041 tactaaaatt ttgttcttta tcatatggat tagatctgat accactcagt ctcaggcact
     8101 tcgaccttct gtatttcttc cctacggacc ttggctccgg actttattac aaacttccca
     8161 agctctccta cgacagttga gttggcagga acatcatgta ttacgacagc atcagcgcct
     8221 atctttgagt tttttcccac agtgaccgct ccaaggactt tgcatccaac acccagcacg
     8281 acattgtcct ctatagtagg atgtctcttt gcaccttttg catcaaccca ccacccatgg
     8341 cctccaagag tcactccgtg atacatcatc acatcgtttc ctatctcagt agtttcacct
     8401 atcacaattc cagcgccatg gtcgataaag aatcttttgc ctatctttgc tccaggatgt
     8461 atctcgatat ttgtaagcaa cctgccaatc tgggatatca gccttgggat gaatggtagt
     8521 ttaagactat aaaaaagatg cgctatccta tgcagcaaga ttgcatgcac tccttggtag
     8581 agtaccacct ctagagcatt tattcccctt agggcaggat ctttcttgta cgctgtgact
     8641 aaagtatcaa acattatcat cgccaaatag tgctgtgctt aaatatcttt ctccatggtc
     8701 tggcaatatc acaacgacct tttttccttt tccaagcttt tttgcgatct gcagagcagc
     8761 ccatgctgca gcacctgaag agattccgac aaatatccct tcctctcgcg ccagtcttct
     8821 tgaagtctca aacgcattat cattagttac ccttatcact tcgtcataca ctttggtgtt
     8881 aagtacgtcc ggtacaaatc ctgcgccgat tccctgtatc tcgtgcggcc caggcgctcc
     8941 gccagacagg acaggagatg catccggctc gacagcgatt atctgaacgg tttttccagc
     9001 ctttttccta agcacctcac caactccagt gattgtgcct cctgtgccaa ttccagccac
     9061 aaacgcatca aggtcaggca cctgctctat tatctcgaca gctgttgtct tgcggtgtat
     9121 ctcaggattt gcaggg
//
LOCUS       DASRUK010000023        13119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_362395, whole genome shotgun sequence.
ACCESSION   DASRUK010000023 DASRUK010000000
VERSION     DASRUK010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 13119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13119
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362395"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /locus_tag="VFF28_00975"
     CDS             complement(<1..1318)
                     /locus_tag="VFF28_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HZX44234.1"
                     /translation="MGVKILESKAAGYKPFFLCVAMIFILSVWIYASDNSQGYFSPAI
                     HGLVSSLNNSDSELPEIIKTNEHKLNDATKRHLNSLAVDYTDKYILRFKKQINENKLD
                     KVNVKKTMPKLRIASIDANRKEAINLLKDSEIESLELDQSIEQLSDNIPYGVYKTNAP
                     PIWNNTKGKGVKVAILDTGISKHPDLSIAGGVSIISEDYIDNNGHGTAVAGVVSALLN
                     DEGLVGVAPETNLYAVKIMEGFYGDLSNAIGGVEWAIDNNMNIISMSFGMEAYSQIFK
                     KVLDEAYANGTLIVAAAGNNGRDILYPAAYSSVIAVGATDENNERASFSSFGADMELV
                     AAGVSINSTSLADTYSLSSGTSMAAPHVAGVAALVWAYNKSLTNKQVRAKLRNDALDL
                     GELGRDDYYGYGLVQANIQSNNLSSVDERYFYEVYNITGYGTPLQEL"
     gene            1492..2778
                     /locus_tag="VFF28_00980"
     CDS             1492..2778
                     /locus_tag="VFF28_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HZX44235.1"
                     /translation="MTKIETKCLHAGQAPDPTTLSRAAPIYRTSSYVFKNAQHAANLF
                     GLKELGNIYTRLMNPTTDILEKRLAELEGGVGGLALASGTCAIFYSIINAARVGDNIV
                     SANDLYGGTYTQFNNILPDLGITTGFVDPLDPDNFKKAINEKTKAVFCETIGNPALNV
                     PDFKAIAEIAHDNGIPFITDSTFTTPALLQPISHGADIICHSLTKWIGGHGTAIGGAV
                     VDSGNFDWQKSKKHPLLTEPDQSYHGIRYAFDLGALNKLAYILRMRLVPLRNLGGCIS
                     PDNSWVFLQGLETLHLRMQRHSENALKVAESLSDNKLVEWVKYPGLNTHPTHDNAKKY
                     LKNGYGGMVVFGIKGGAKAGEKFIDNLKVFSHLANVGDAKSLAIHPTSTTHSQLSEEQ
                     RVAGGITPELVRLSIGIENVGDILEDIEQALKASQR"
     gene            2782..3936
                     /locus_tag="VFF28_00985"
     CDS             2782..3936
                     /locus_tag="VFF28_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZX44236.1"
                     /translation="MSFETDAIWKGQVPVISEDSFGDVTVPIHMSSTYARKKVFELPG
                     GYEYSRCGNPTRSAYEECLASLEQGKYGISCSSGLAAVTAVCLLLKQGDNIVAFEDIY
                     GGTLRIFNDILPKAGITTTYFDATKPETIISAIKENTKLIWLESPTNPLLRVCDIKKI
                     SELAKQKNKDILVVVDNTFLSPYFQNPLACGADIVVHSTTKYVGGHSDLIGGAIICSS
                     QELNEKIKFIHNAIGLNPSPFNDWLCLRGLKTLAVRMEQHQINTLAIARFLKSHPSVE
                     GVFYPGLEQAYKGFESQATGHGGVVSFKIKGTKEDAIRFVESTKIFALAESLGGTESL
                     IEHPVTMSHSSVPPEKLKKLGVTDNLIRLSVGIEDANDLIADLKQARWSN"
     gene            3924..5024
                     /locus_tag="VFF28_00990"
     CDS             3924..5024
                     /locus_tag="VFF28_00990"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HZX44237.1"
                     /translation="MVKLAKKLFKITEDIELECGKKLGSIVLAYETFGTRNKDKSNAI
                     LIFHALSGDSHVCESEGKPGWWDEHVGPDKSFDTNKYFIICANVIGGCQGSTGPSSIN
                     PATGRQYGASFPIITIKDMVIAQKKLIDSLGIKKLFAVTGGSMGGMQLLQWLICFPDS
                     TKLVIPIATAAKQSPQNIAFHEAGRKAIIADPNWNKGDYYMKSAPALGLAVARMIGHI
                     TYLSENAMREKFGRRLQGKEKVDFTFDTEFQVEGYLEHQGLKFVERFDANSYLYITRA
                     IDYFDLTEGGEKQLVDALKGINSKFLVLSISSDWLYATADSLEIVRALLGNNLDVTFY
                     EVSSDDGHDGFLLKNKTQESLIKNFLNNNYEG"
     gene            5026..5622
                     /gene="metW"
                     /locus_tag="VFF28_00995"
     CDS             5026..5622
                     /gene="metW"
                     /locus_tag="VFF28_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HZX44238.1"
                     /translation="MENRFDYKVIIKEIEEGSKVLDLGCGSGELLSCLKKHKKIDARG
                     IELKQDKVRQCIEHGLSVLHGDIDEGLKHYGDKSFDYVVFCETIQVVRNSLLVLEEAL
                     RVGKKVIVSFPNFGHYTIRFPYFFFGKMPKSSSLPYEWYNTPNIREVTIKDFRTLCRT
                     HNIKIINEADFLKNSGKSKKISFWPNLLASNSIFIMEK"
     gene            complement(5655..6014)
                     /locus_tag="VFF28_01000"
     CDS             complement(5655..6014)
                     /locus_tag="VFF28_01000"
                     /inference="COORDINATES: protein motif:HMM:NF018621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1284 domain-containing protein"
                     /protein_id="HZX44239.1"
                     /translation="MDLRAHHLLCMRYFKGKGYSKGFVSNFRKVLKRLKKERFVLVDY
                     PDTICEACPHLIGERCAKKPGSERKVKGKDDKFLRIMGLKSRKQIYLIEAERLVSLKL
                     ERLRKTCKHCEWKDWCK"
     gene            complement(6005..6715)
                     /locus_tag="VFF28_01005"
     CDS             complement(6005..6715)
                     /locus_tag="VFF28_01005"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HZX44240.1"
                     /translation="MANLFGQYQYKDTFIHRLDARTKIILLIGLSIILFLPAVPFAFM
                     IIFIILLLFLSRMSIKNLIRNLRPFLFFFTFILIMYLLFSREDIHKGIASIWRFLLLI
                     ALSSILTYSTTTSELVRAIERLFAPLRLIRISPRNIAVMVSATIRFIPVLLSEAGKVR
                     DAQGSRGASLRKPKHVAGFANTLIRKTFSRAANLADAIQSRCYRDKNHTHFVDLRLKS
                     GDVIAMLAIIFLLGALWT"
     gene            complement(6708..7397)
                     /locus_tag="VFF28_01010"
     CDS             complement(6708..7397)
                     /locus_tag="VFF28_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZX44241.1"
                     /translation="MIELKNISFVYEQRQILTDISLKISEGESVALLGHNGSGKTTLI
                     KHLNGLLMPSKGEVIVDGLSTKKNDWEIKKKVGFVFQSPEDQLINSIVEEDIAFGLEN
                     LGVAPIEMRKRVDAILNALEISTLAKENVNNLSFGQKQLVALAGVLVMEPKYVVFDEP
                     SSMLDPKNKANLRKIISNLIKGGTTVLLVTQNLEDILGFKRIIILDQGRIVFDGKKLS
                     RAVLKKSGLYG"
     gene            complement(7394..8200)
                     /locus_tag="VFF28_01015"
     CDS             complement(7394..8200)
                     /locus_tag="VFF28_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44242.1"
                     /translation="MASQMILFDILSFSCMNCRRSLQHILKLDARYRGLGLRTILLHP
                     PEWRFEDKKELVLGFLKKNKINLPLVMDKDKKKIARLGIDFWPAQVLLLKGRIIYKHI
                     GEGKYSQLEDAIRRALKAKREKIFKKEPRFTRYPCIYFGKNKGSGKYLAEGKWKASEE
                     AIEGYGIINLVTRGKKIYIVAEANKNPNRRGIFSVVNTLNVESHNNNPVTQVSGVFTT
                     NKPSAALVRGNGARISTPGLYLVKTFRSDKKRKFKLNVKKPVKLYSLAAE"
     gene            complement(8272..9033)
                     /locus_tag="VFF28_01020"
     CDS             complement(8272..9033)
                     /locus_tag="VFF28_01020"
                     /inference="COORDINATES: protein motif:HMM:NF014669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HZX44243.1"
                     /translation="MSKNYIKGMMFAALFAALTAAVSPIKIPLGFTPVPITLQTLMVL
                     LSGALLGAYLGALSQFLYLLVGALGLPVFAGGSFGLGAILGPTGGYLMSYPIAAFVVG
                     FIYQKRNKAVQYISAGLIGCLMLIAYTDLIFNAGIMKYGKATLVSLMTFGQRMAFVFG
                     SLAVIAIIFYLTFYLHKKKKIEFNSVFAMFAGTIVIYAFGASYGKLITGLGWAAVFIG
                     WVLPFIIGDSIKLMVAAYIANNADAKRYCEKLRSK"
     gene            complement(9362..11278)
                     /locus_tag="VFF28_01025"
     CDS             complement(9362..11278)
                     /locus_tag="VFF28_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZX44244.1"
                     /translation="MILSIVSAAEHTWPLISGNLRHTGLSPYDTSNINGAIKWTFDTN
                     DGIESSPTIAEDGTVYFGSHDGYLYALNPDGTLKWKYKPAEPVYDERWKHYSAIISSP
                     AIAKDGTVFIIAPDDFLHAVKEGKERWRFPLKWNGIDFWSSAVIGADGTIYVGSARTD
                     DDSQLVAGFYAINQDGKEKWHYEIDAGVPSSPAIGKDGSIYVGGNIMKKVGSSATTEG
                     YLFAFKPDGTLKWKFKTQDWLESSPSIAEDGTLYTGSKEGRIYALDAEGTKKWEYATG
                     DGISGTPAIAKDGTLFIGSWDNNMYALLPDGKLKWKVQVEEGFESLGASAVIGADGTI
                     YFGSSRGFLYAYNPDGTEKWRLSNLGSVASTPAIGKDGMLYFGAWNKKFYAVGKGDAP
                     KIKEGEGQLECHEKQLKREVEFDKNPINTCIDGKCDSYEMKCQRKGQNGKCYAPIEVC
                     VSKSCKKYRSYCNLIIKNTEPGRGFTTMFNENYVTADGKKQFIKEMKAFLDIGQERSI
                     VWQYEVDANNAGQCDYSDLRNPICEKKEEEVTWNFTTERSDVQGGDKAPPQECMVGGK
                     FIGEDRCRALFDKGRDAPPEKCLVDGKFIGEEKCRALMDVKSEGPKKDCAGQCGNKDQ
                     SIVKKIISWFLSIF"
     gene            complement(11513..12415)
                     /locus_tag="VFF28_01030"
                     /pseudo
     CDS             complement(11513..12415)
                     /locus_tag="VFF28_01030"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(12446..12673)
                     /locus_tag="VFF28_01035"
     CDS             complement(12446..12673)
                     /locus_tag="VFF28_01035"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZX44245.1"
                     /translation="MTTRRRFDREFKLQIIREAEQKPMAELCREHNLNSTVVCRWKKE
                     LEQYPKDAFKGRGNLYKIEARLCSASNRPVV"
BASE COUNT         3846 a         2960 c         2532 g         3781 t
ORIGIN      
        1 gaagctcctg caatggcgtc ccatagccag tgatattgta cacttcatag aaatagcgtt
       61 catcaacgct gctaaggtta tttgactgga tattagcttg gacaagccca taaccataat
      121 aatcgtccct tccaagctca cctaaatcta aggcatcgtt ccttaacttt gcccttacct
      181 gcttattagt aagtgattta ttataagccc ataccaaagc tgcaacccct gcaacatgag
      241 gtgctgccat ggatgttccg cttgacaagg agtaggtatc agccagagag gttgaattga
      301 tgcttacacc agcagcaaca agctccatat cagctccaaa agaggaaaat gacgctcttt
      361 cattattttc atcagttgca cctacagcga ttacagaaga atacgcagct ggatacaata
      421 tgtcccttcc attattacct gcagcagcta caattaaggt tccattagca taagcctcat
      481 ccaatacttt cttgaatatc tgtgaatatg cttccattcc aaaactcata gagataatat
      541 tcatattatt atcaatagcc cattcaacgc caccaattgc attggataag tctccataaa
      601 atccttccat aatctttacc gcataaaggt tagtctcagg tgcaacaccg accaatcctt
      661 catcgttaag caaagcagaa acaactcctg caacagcagt tccatgccca ttattatcaa
      721 tatagtcttc actgataata gacactccac cagctatgct taaatcagga tgcttgctta
      781 ttcctgtatc tagtatagct actttaacac cttttccctt agtgttgttc cagatagggg
      841 gagcattagt tttatagact ccataaggga tattatctga taactgttcg atagattgat
      901 caagctccag tgattcaatc tcagaatctt ttaataggtt tattgcttcc ttcctgtttg
      961 catcaatact agcaattctt aattttggca tcgttttttt aacgttgact ttatctagct
     1021 tattctcatt tatttgcttc ttaaatctaa ggatatattt gtcggtgtaa tcaactgcca
     1081 gggagttaag gtgtctcttt gtcgcgtcgt tcaacttatg ctcatttgtt ttaataatct
     1141 ccggtaattc gctatcagag ttgtttaaag aagagaccag cccatggatt gcaggagaaa
     1201 aatacccttg agagttatct gaggcatata tccaaaccga tagaatgaag atcatagcaa
     1261 cacatagaaa aaaaggctta taacctgctg cttttgactc taaaattttt acccccaact
     1321 caataaatgc aaccgctcct atataaaacc ttgtaacgac cgctgggtaa ctggtagttt
     1381 ataaatacat ataaaaatct gacagattcc cgcttattcc aaaaggaata aatcattcaa
     1441 caagatattt aaacaattaa cggatgtaaa cactttctga ggtggataaa tatgacaaag
     1501 atagaaacta aatgcttgca tgcagggcaa gcccctgacc cgacaacgct ttcaagagca
     1561 gcgcctattt acaggacaag ctcttatgtt tttaagaacg cacaacatgc ggcaaacctg
     1621 tttggcctaa aagagctagg caatatctac acaaggttga tgaaccctac tactgacatt
     1681 ttggaaaaaa ggctagccga gcttgaagga ggagttggag gccttgctct cgcgtccgga
     1741 acatgcgcaa tattttattc gataataaac gcagcaagag ttggagacaa catcgtctct
     1801 gcaaacgacc tctatggagg cacatacacc cagtttaaca acatccttcc tgaccttggc
     1861 ataacgactg gatttgtaga tccgctggac cctgacaatt tcaagaaagc cataaacgaa
     1921 aaaacaaagg cagttttctg tgagaccata ggaaatccgg cgctgaacgt ccctgacttt
     1981 aaggcgatag cagagatagc gcatgacaac ggaattccgt tcataacaga cagcacattt
     2041 accacccctg ctttacttca gcccatcagc catggagcag acatcatctg tcactccttg
     2101 acaaaatgga taggaggaca cggtacggcg attggcggag ctgttgttga ctcaggcaac
     2161 tttgactggc agaagagcaa aaagcatccg ctcctcaccg agccagacca aagttatcat
     2221 ggcataagat atgcatttga tcttggagca ttaaataagc tagcctacat ccttaggatg
     2281 aggcttgtgc cgctaagaaa ccttggaggc tgcatctcac cagataactc ttgggtattc
     2341 ctgcaggggc tggaaacact gcacttaagg atgcaaaggc attcagaaaa tgccctaaag
     2401 gttgcagaaa gtctaagcga taacaagctt gtagaatggg taaaataccc cggactgaac
     2461 actcatccaa cacatgacaa tgcaaagaaa tacctaaaaa atggatacgg cggaatggtt
     2521 gtctttggca tcaaaggagg agcaaaagca ggagagaagt ttatagacaa tctgaaagtc
     2581 ttttctcacc ttgcaaatgt aggggacgca aaatcgcttg ccatccatcc gacatccacc
     2641 acccactctc aattaagcga agaacagaga gttgcaggag gaatcacccc tgaattggtg
     2701 agattatcga taggcatcga aaacgttggt gacatactgg aagacatcga gcaagccctt
     2761 aaagcttcac agaggtagaa aatgagtttt gaaacagacg cgatttggaa aggccaagtg
     2821 ccagtcatct cagaggattc ttttggagat gtgactgtgc caatccacat gtcaagcact
     2881 tacgcaagaa agaaagtctt cgagctgccc ggaggctatg aatatagccg ctgcggaaat
     2941 cctacaaggt ctgcttatga agaatgttta gcttcacttg agcaaggaaa atatggtatc
     3001 tcctgttcat caggccttgc tgcagtgaca gcagtgtgtc ttctcctaaa acaaggagac
     3061 aatatcgtcg cctttgaaga tatctatggc gggacgttaa ggatattcaa tgacatcctt
     3121 ccaaaggcag gtataaccac tacatacttt gatgcaacta aaccagagac tataataagt
     3181 gcgataaaag aaaacacaaa actaatctgg cttgaatctc ccacaaatcc attgctgagg
     3241 gtctgcgaca taaaaaagat ctcagagctt gcaaagcaaa agaacaagga catccttgtt
     3301 gttgtcgaca acaccttcct tagcccttac tttcaaaacc ctttggcatg cggagcagat
     3361 atcgttgtcc attccactac aaaatatgtc ggcggacatt cagacctgat aggtggagcc
     3421 ataatctgca gcagccagga gctgaatgaa aagataaagt tcatccataa tgcaataggc
     3481 ctaaacccat cgccattcaa cgattggctt tgcctaagag gcctaaagac actcgcagta
     3541 aggatggaac agcatcagat aaacacactt gcaatagcaa ggtttttaaa atcacatcca
     3601 agcgttgaag gggtgttcta tccaggacta gagcaagcct acaagggttt tgaatcgcag
     3661 gcaacaggac atggaggcgt tgtctctttc aagataaaag gcacaaaaga agatgctata
     3721 cgtttcgtag agtcgacaaa aatatttgcc ctagcagaaa gtcttggcgg gacagaaagc
     3781 ctaatcgagc atcctgtcac catgtcgcat tcatccgtcc ctccagaaaa gctaaaaaaa
     3841 cttggagtta cagacaatct catccggctg tctgtcggta tcgaagatgc aaatgatctt
     3901 atcgctgatc taaaacaggc gaggtggtca aactagcaaa aaaattattc aagataacag
     3961 aagacatcga gctggaatgc ggcaaaaagt taggcagcat agtcttagcc tatgagacat
     4021 ttggaactag aaacaaagac aagtctaatg ctatactcat ctttcatgct ctgagcggcg
     4081 actcccatgt ctgtgagagt gagggaaaac caggttggtg ggacgagcat gtcgggccag
     4141 acaagtcatt tgatacaaac aagtatttca taatctgtgc aaacgttatt ggcggctgcc
     4201 agggatctac tggtccttct tcgataaacc ctgcaacagg cagacaatac ggagcaagtt
     4261 ttcctatcat cactattaag gatatggtaa tcgcccaaaa gaagcttatt gattcactcg
     4321 gcataaaaaa attatttgca gtgacaggag gctctatggg aggcatgcag cttttgcagt
     4381 ggctcatctg ctttccagac agcacaaagc ttgtgatacc aatagccaca gcagcaaagc
     4441 aatctcctca aaacatagca ttccatgaag caggaagaaa agcgataatc gcagatccta
     4501 actggaataa aggagactat tacatgaagt cagcccctgc cttaggtctt gctgtcgcaa
     4561 ggatgatagg ccatataaca tatttgagcg aaaacgcgat gagagagaag tttggaagaa
     4621 gactgcaggg taaagaaaaa gttgatttca catttgacac agagtttcag gtcgaaggct
     4681 accttgagca tcaaggccta aaatttgtag agaggtttga tgcaaactct tacctgtata
     4741 taaccagagc catcgactat tttgatctga ccgaaggagg cgagaagcag cttgtagatg
     4801 cattgaaagg gataaattca aaattccttg tgctttccat ctcatcagac tggctgtacg
     4861 cgacagcaga ttcacttgag atagtaagag cgcttcttgg aaacaacctg gatgtgacct
     4921 tttatgaagt tagttctgat gatggtcatg atggattttt actaaaaaac aaaactcagg
     4981 aaagccttat caaaaacttc ctgaacaaca attacgaggg ctagcatgga aaacaggttt
     5041 gactacaaag tcataattaa ggagattgaa gaaggatcaa aggtccttga ccttggctgc
     5101 ggctcaggtg aactcttaag ctgcctaaaa aagcataaga agatcgatgc aagaggtatc
     5161 gagctaaagc aagataaggt caggcagtgc atcgagcatg ggctttctgt gcttcacggc
     5221 gatatagatg aaggcctaaa acattatggt gataaatcct ttgattatgt tgtattttgt
     5281 gagaccatcc aggtcgtcag aaactctctg ctggtgcttg aggaagcgct aagagtagga
     5341 aagaaagtga tagtaagttt cccaaacttc ggtcattaca cgattaggtt tccatatttc
     5401 ttctttggta agatgccaaa gtcaagctct cttccttatg agtggtataa cacgccaaat
     5461 atcagggaag tgacgataaa agatttcagg actctttgta gaacgcacaa tatcaagata
     5521 ataaatgaag ctgactttct aaagaacagc ggcaaaagca aaaaaataag tttttggcct
     5581 aacctccttg cttcaaatag catattcatc atggagaaat agcggttatt atcttcgatc
     5641 gatagctatc cttgttattt gcaccagtct ttccattcac agtgtttaca ggtttttctt
     5701 aacctctcca actttaagga aactaaccgt tctgcctcta tcaaataaat ctgctttcgg
     5761 ctttttagcc ccatgatgcg caaaaactta tcgtcttttc ctttaacttt tctttctgaa
     5821 ccgggctttt ttgcgcacct ttcacctata aggtgagggc acgcttcaca gatagtatca
     5881 ggataatcga ctaggacaaa tctttccttt tttagcctct ttaagacctt cctgaaatta
     5941 gagacaaaac ccttgctgta tccttttccc ttaaaatacc tcatgcaaag aagatgatgg
     6001 gctcttaggt ccataatgca cccagcaaaa agattatagc aagcatagct attacatctc
     6061 cactttttaa tcttagatcg acgaagtggg tatggttttt gtccctatag caccttgatt
     6121 gaatagcgtc agcaaggttt gcagccctgg aaaatgtttt tcttatcagg gtgtttgcaa
     6181 atccagcaac atgttttggt ttcctaaggc ttgctcctct tgagccctgg gcatctctga
     6241 cctttcctgc ctcagaaagg agtacaggga taaaccggat tgtagcagag accatcactg
     6301 cgatgtttct tgggcttatc cttatcagcc tgagcggcgc aaacaatctt tctattgccc
     6361 tgacaagctc gcttgttgtt gtagaatacg tcaggattga ggataacgcg ataagcaaaa
     6421 gaaacctcca tatggaggca atgcctttat gtatatcctc tctcgaaaac aaaagataca
     6481 tgatcaggat aaaagtgaag aaaaacagaa aaggcctgag gtttcggata agatttttta
     6541 tgctcatccg agacaaaaag agcaaaagaa tgataaaaat gatcataaaa gcaaatggaa
     6601 ctgctggcag aaacaggatg atgcttaagc caataagcag tattatcttt gtccttgcat
     6661 ccaatctgtg gatgaatgta tctttatact gatactgtcc aaagagatta gccataaaga
     6721 cctgactttt ttagcactgc cctgcttagc tttttgccgt caaagactat cctgccttgg
     6781 tcaaggatta ttattctctt aaatcctaag atatcctcaa gattttgggt tacaagaagt
     6841 acagtagtcc ctcccttgat tagattgctg attatcttcc ttagattagc tttgttcttt
     6901 gggtcaagca tcgagctagg ctcatcaaat acaacatatt ttggctccat cacaagcact
     6961 ccagcaaggg cgacaagctg cttctgccca aaagaaaggt tgtttacatt ctccttggct
     7021 agagtagaga tctccaaggc attcaatatg gcatcgacac gcttcctcat ttcaataggg
     7081 gcaaccccca ggttttcaag cccaaaagcg atatcctcct caacgattga attgatcagc
     7141 tggtcttcag ggctctggaa aacaaaccct acctttttct ttatctccca atcatttttc
     7201 ttagtgctaa ggccgtcaac aatgacctcg cctttgcttg gcatcaataa tccgtttagg
     7261 tgctttatca aagttgtctt gccagagccg ttatggccaa gtagcgcaac agactctcct
     7321 tcagatatct taagagatat gtctgtaagt atctgcctct gttcatagac aaaacttatg
     7381 tttttgagct ctatcattct gcagcaagcg aatatagctt gaccggtttt tttacattta
     7441 gtttgaactt ccttttttta tcagacctga aagtttttac caggtaaagg ccaggcgtgc
     7501 ttattcttgc tccattccct cttactagag cggcgcttgg tttatttgtt gtaaataccc
     7561 cgctcacttg cgtgactggg ttattgttat ggctctccac gttgagggta tttacaacac
     7621 taaaaatacc ccggcggttg ggatttttat ttgcctctgc gacaatgtat atctttttgc
     7681 ctcttgtcac aaggttaatg atgccatatc cttctattgc ttcttctgat gccttccatt
     7741 taccttcagc cagatatttg ccgcttcctt tattttttcc aaaataaatg cagggatatc
     7801 ttgtaaacct tggctccttt ttaaagattt tttctctctt tgccttaaga gctcttctga
     7861 tagcatcttc aagctggcta tattttcctt ctccgatgtg cttgtatatt atcctgccct
     7921 taagaagcaa aacctgagca ggccaaaaat caatacccag acgagctatc ttcttcttat
     7981 ctttatccat gaccaaagga agatttatct tattcttctt aagaaagccc aagaccaact
     8041 cttttttatc ttcaaaacgc cattctgggg gatggagaag tatcgttctt aaaccaaggc
     8101 ccctgtatct tgcatctagc tttagtatat gctgcaagga cctcctgcag ttcatgcagc
     8161 taaaagagag aatgtcaaaa agtatcattt gagatgccat aaaccgcttt tggcgcactg
     8221 gtgtaagccg gcgatttctg aagcattgtc tcaaaaactc cgcttcactc ttcatttcga
     8281 tcggagtttt tcacaatatc ttttggcatc tgcattattt gcgatatatg cagctaccat
     8341 taacttgatg ctgtcgccga tgataaacgg aagcacccat ccgataaaaa cagcagccca
     8401 gccaaggccg gtgataagct ttccgtatga tgctccaaaa gcatatatga cgatagtgcc
     8461 tgcaaacatg gcaaagacag aattaaattc aatttttttc tttttatgga gataaaaagt
     8521 taagtagaat attattgcaa tcactgcaag gcttccaaaa acaaatgcca tcctttggcc
     8581 aaatgtcatc aacgacacta aagtagcctt tccatatttc attatgcctg cattaaaaat
     8641 tagatcggta taggctatga gcatcaggca ccctataagg ccggcagaga tatattgaac
     8701 tgccttattt ctcttctgat agataaatcc gacgacaaaa gctgctatcg gatagctcat
     8761 caggtatcct cctgtaggcc caagaattgc tccaagccca aagcttcccc ctgcaaacac
     8821 aggaagcccc agcgctccga caagtaggta aagaaactgt gaaagtgcac caagataggc
     8881 tccaagcaat gctcctgaca aaagcaccat caatgtctgc agtgttatag gtacaggagt
     8941 aaatcccaag gggatcttta tagggcttac cgctgctgtc aaagctgcaa ataaagcagc
     9001 aaacatcatt cctttaatgt agtttttaga catcttatcg cctcctggct gccttcaatt
     9061 ggctgcgtat gtcatttatt gcatttagtt ataaatcttc tggtcgttga tcacagcctt
     9121 aacttcctcg catatgttgt ttttttttga agactccgcc gtttagcttc atcaaagcaa
     9181 gcttttatcc cgcttaggaa tatccgccga aataggaccc atccttgtaa gccctgcatc
     9241 gcctcaaaac aagtttttaa tattacaata tattctaccc ctaaatggac atcaaggata
     9301 tctggattac aaagaaggag gtgtaatgat caacaccctg ccgcaagaga gagttagatg
     9361 actagaagat agataaaaac caagagatta ttttttttac aatgctctgg tctttgtttc
     9421 cgcattgtcc ggcgcaatct tttttcggac cttcagattt tacatccata agagccctgc
     9481 acttctcctc gccgataaat ttgccgtcta ctaagcactt ttcaggcggt gcatcacgtc
     9541 ccttgtcaaa caatgccctg cacctgtctt cgccgatgaa tttgccaccc accatgcact
     9601 cctgaggcgg cgctttatca ccaccttgaa catcactgcg ctctgtagta aagttccagg
     9661 taacttcctc ctcttttttc tcacatatag ggttcctaag gtcgctatag tcgcattgcc
     9721 ctgcattgtt tgcatctacc tcatactgcc atacaataga ccgttcttgc cctatatcaa
     9781 gaaaagcctt catctctttg atgaattgct tctttccatc agcagtcaca tagttctcat
     9841 taaacattgt ggtaaatccc ctgccaggct ctgtattctt gattataaga ttgcagtaag
     9901 agcgatattt cttgcagctt ttggaaacac acacttcaat tggtgcataa cacttgccat
     9961 tctggccttt tctttggcac ttcatctcat aagaatcgca cttcccatcg atgcaagtat
    10021 ttatcggatt tttatcaaac tcaacttctc gcttaagctg cttttcatgg cactccagct
    10081 ggccttcccc ttctttaatt ttcggagcat ctcctttccc cacagcataa aacttcttgt
    10141 tccaagcgcc aaaataaagc attccatctt tgccgattgc aggggtggag gccactgatc
    10201 caagattaga aagccgccat ttctctgtcc cgtcaggatt atatgcatac aaaaaacctc
    10261 ttgacgatcc aaaatagata gttccatcag ctccgataac agcagaagcg ccaagcgatt
    10321 caaagccctc ttcaacctgg actttccatt taagcttgcc atctggaagc agcgcataca
    10381 tattattatc ccaagagccg ataaatagtg tcccgtcttt agctattgca ggagttccgc
    10441 ttatgccatc accagtagca tattcccact tctttgttcc ctctgcatcc agtgcatata
    10501 tccttccttc ttttgagcct gtatagagag tgccatcttc agcgatagat ggagatgatt
    10561 caagccaatc ctgggtctta aatttccatt tcagcgtgcc atctggcttg aacgcaaaga
    10621 gatatccctc agttgtggct gaactcccta cctttttcat gatgtttcca ccgacatata
    10681 tcgagccatc ttttcctata gcaggagagc ttggcactcc tgcatcaatt tcataatgcc
    10741 atttttcctt accatcttga tttatagcat aaaaacctgc aacaagttgg ctatcatcat
    10801 ctgtcctggc cgacccaacg tagatagttc catctgctcc gataacagcc gatgaccaga
    10861 aatctatgcc gttccattta aggggaaagc gccatctctc cttgccttct ttgacagcat
    10921 gaagaaaatc atcaggagca ataataaata ctgtaccatc ttttgcaata gcaggcgatg
    10981 agataattgc actgtaatgc ttccatctct catcataaac aggctctgcg ggcttatact
    11041 tccactttaa agtaccgtct ggatttaagg catagagata accatcatga gaaccaaaat
    11101 agacagtgcc gtcttcagct attgttggtg agctttctat gccatcgttt gtatcaaaag
    11161 tccactttat tgcgccgttg atattgcttg tgtcataagg gcttagcccg gtgtgcctaa
    11221 gattgccgct tatcaatggc catgtatgtt ctgcggcaga gacgatgcta agtatcacaa
    11281 taaatgcaaa tggaaaaagt atagtttttt tgataacaat aacctcttaa gcacttcttt
    11341 tccttaccac tatttaaata ttttccagga ttaaagtaca aaattgccca gcatttatac
    11401 caaaaaacct catcgttatc taatttgctt gcaaaatatg aagcatcaat aatacgcaag
    11461 aagataacct ttgtaagccc tttggagtga acccctataa gttcacagta agttaagtca
    11521 caatagcggt atataaaccc tccttctttt gctcaaattc agctggcggc atgtatccga
    11581 tgccgctatg aagccttttc tcattgtaga cttcttcaat aaatctctgg atgtttatgc
    11641 ggacatcctc taatgtttca tactcattca tatacacctc ctcatatttg atggtcttta
    11701 tgaagctctc agcgtaggca ttctcgtaag aattgcctac ctctcccatg cttggcttca
    11761 ttcctgcctc ccgtaagagt tcaacatatg ctgttgcagc gtattgaacg ccctgatctg
    11821 agtggtggat gcaacctctc aagccatctc ctctcagatt aatagccatt ttcagggcat
    11881 ccatggtcag ctgagcatca ggattaaggc ttaatgccca tccgatacac cgtctgctga
    11941 acagatccat gattacggca aggtagacaa atccacacag caatcgtatg tatgtaatgt
    12001 cacttaccac tgcttgattg atatctgtca ataccaaatc cttaacaaga tttggatact
    12061 tctttaggcc gtggtttgat tgtgtcgtct tgggtgtaaa tttcttctgc cttacaagca
    12121 ggttttcttc cttcattatg gagaggacac gtttgctgtt tgctactatt ccattcctgc
    12181 gcaaagccca agtcattctc ctatagccat attttgggaa ttcaagagca atctcatgga
    12241 tatcctttct taacgaggca ttggtatcga ttggcccctt cctttgcttc catttatagt
    12301 aggctctttg gcttacgcta agcgtctttg aggatatgct tacaggcagc tgactttctc
    12361 tgatcattct gtacacctga gcattctttt ctctgcctgc ctttcctgca aggactgaat
    12421 ggcttttttt aaaagggcat tctcctcata caactggcct attagacgct gagcaaagcc
    12481 tggcctcaat cttgtataga ttgcctctgc ccttaaaggc atccttagga tattgttcaa
    12541 gctctttttt ccatctgcag accacagtgg aattgaggtt atgctcacgg caaagttcag
    12601 ccattggctt ctgttctgcc tcccttatga tctgcaactt gaattctcta tcaaatcttc
    12661 ttctggttgt catggtattc ctccttatca taccacacta ctaaaactta actatttgag
    12721 aactaacagg ggtgcagtcc acaatgctgt aagcgatgga atcggcagcc tctataatgt
    12781 atctaccaag gttaaactgc cttatccatg atatgttatt attaaagagt ggtaataatg
    12841 gtaaggctta aatattagaa tgcttttggt ttgtaagaaa tgaacatccc catagggcaa
    12901 actggcctgg agaggataat cgcagaggta aaccaggaaa tatttaactt gagagccatt
    12961 ttttttctaa ttacctctct agtagtctga caaattaaat tagttacttt tatatattag
    13021 ctccttctta ataattgata aaatgaaaac cccccagata aagctaaaaa caaaagaatg
    13081 taagttctta caggagtttg tcaagaaagg aacaaagaa
//
LOCUS       DASRUK010000024         4578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_857692, whole genome shotgun sequence.
ACCESSION   DASRUK010000024 DASRUK010000000
VERSION     DASRUK010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857692"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..1831
                     /locus_tag="VFF28_01040"
     CDS             197..1831
                     /locus_tag="VFF28_01040"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HZX44246.1"
                     /translation="MLHLKREVRDIKRFNQILFILAEEGFGYLFQRFKKKELREHEKP
                     RRLVKAFERLGPTFIKLGQILSLRPDLVPKEYLKALGELQDKVPPFPLKVAEKIIKEE
                     LGKPIPQLFKKFDKKPIASASVSQVYKAVLKTGETVAVKIQRPGIKQQMETDIEIMRY
                     LTKLIEKHSKLVQTIDPLKLIDEFEEWTERELDFSNEAENARKFHENFKDSKTVKIPK
                     VYDSLSTRKVLILEFIEGVELHYLDKLKEKKGYNIKAIMKNGFDSILTQVFIHGFFHA
                     DPHPGNILVLKNNKISFIDFGIVGYFDESLKKKSIELFYGIIEEDMDAVADAFLDMGM
                     VDEEATNIESFKREIKLIVDSLQKTKLKDVKISYVLEKVLDVCFKHKVKMPIDFVLFG
                     KAIVEFEGIGLEYEPDFVFSDAAKPFLEKLIKKQFSVKTMAKELQKNALRYGKFLRDL
                     PDQVSSVLKKVQKGTIKIDVQDTDVKQLGRDIDRSSNRLTYGLIIAAFLITGALLVDV
                     GKPGFYGYSMISVLSFGLALMFLALLIISILNEVKQ"
     gene            1828..2154
                     /locus_tag="VFF28_01045"
     CDS             1828..2154
                     /locus_tag="VFF28_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44247.1"
                     /translation="MKQVTKKSLLTAIGAASIAAENAQKILKDISKKGLISAKETRKI
                     VSSLVKETEKERKKIVKIVSKELKDSAKKAEPYLRNKVRTAVSSLKKAQRKMENKGKA
                     VVKKLR"
     gene            2156..2665
                     /locus_tag="VFF28_01050"
     CDS             2156..2665
                     /locus_tag="VFF28_01050"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HZX44248.1"
                     /translation="MPFAVTHVILTIVALDIIRDYIIKDRKTFPLHYLFIGGVAGLLP
                     DIDIPLFWLLDALGISVSWFHRTFTHSLLFAFAFMLVTVTLFLYSKKRKHWLLAAVAT
                     FGVSFHLLLDGLLGGYIMLFYPFSTIGFGIDLAQYITMPAIWQGIDALILLVWLWDLD
                     RRHNLRDYM"
     gene            2723..2965
                     /locus_tag="VFF28_01055"
     CDS             2723..2965
                     /locus_tag="VFF28_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HZX44249.1"
                     /translation="MKTVSADERGRITLGSDMLDRYGKKFIVIAAEKEIVLVPIAEDP
                     LGELKKIGKSAGIDSFTFKELKRMAKEEASKEALSG"
     gene            2968..3336
                     /locus_tag="VFF28_01060"
     CDS             2968..3336
                     /locus_tag="VFF28_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012965895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HZX44250.1"
                     /translation="MFADTDFILGLLKDSDWLKENAVKILDGYKGKIKTSISVMIEVA
                     LICKRLNMDVLNTFAHIFEIIIVDEKTQEVCLRAALYITKYGLGVFDSFNAAFSINDK
                     IISSDSVYDTLGLARLNLRG"
     gene            complement(3337..3996)
                     /gene="pyrH"
                     /locus_tag="VFF28_01065"
     CDS             complement(3337..3996)
                     /gene="pyrH"
                     /locus_tag="VFF28_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HZX44251.1"
                     /translation="MEKVVLSLGGSVIIPDKFDISFVRRLGELIKKHKGKEFIVICGG
                     GKLARNLMANARKINGITPKELDWIGIYATRINALTLKKILGIKGNIITNPTKKIEEK
                     ERILVAAGWKPGFSTDTDAVLLAKNIKAGTVINMTNVDYVYDKDPKISGAKPIEKISW
                     KALRQLVGSKWKPGLNMPFDPIAAREAEKLGLKVVIVGKDLKNLEKVISGKKFKGTVI
                     E"
     gene            4106..4390
                     /locus_tag="VFF28_01070"
     CDS             4106..4390
                     /locus_tag="VFF28_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44252.1"
                     /translation="MAIVHGMDKESSSILASMKKKHAQIESEVEHHLEKKELCLPVSI
                     FSENLGMLESAVIYLRDTMGLRFTEIAKLVKRDYKTVWASYTNGKIKSKR"
BASE COUNT         1475 a          875 c          946 g         1282 t
ORIGIN      
        1 tcataacgtc taagctcttt tggggtaagt agtatttgct ccattatatc accaaacaag
       61 gcctaagaat tacttatatt tatagtttac ttttattgtc gtaaaattta gatgttatag
      121 tacaattttc gcactgcatg attttgcagt tttccttaac cataacttta tatataatct
      181 atctcaaaat taccctatgc ttcatctaaa aagagaggtt agagatatca agagatttaa
      241 ccagatcctg tttatcctgg cagaagaagg tttcggttac ttattccaaa gattcaaaaa
      301 aaaagagctc agagagcatg agaagccaag gcgtttggta aaagccttcg agcggctagg
      361 cccgaccttc atcaagcttg gacagatact tagcctcagg ccagaccttg ttccaaaaga
      421 atacctaaag gcattaggcg aacttcagga caaggttcct ccattccccc ttaaggtagc
      481 cgagaagata ataaaagaag agcttggaaa acccataccc caattattca agaagttcga
      541 caaaaagcct attgcctcag cttcggtctc tcaggtgtac aaagcagtgc taaagacagg
      601 agagacagta gcagtaaaga tacaacggcc gggaattaag cagcagatgg agacagatat
      661 cgagataatg cgatatctta caaaattgat agagaagcat tcaaaacttg tccaaactat
      721 cgatccgtta aagcttatcg acgaattcga ggaatggaca gaaagagagc ttgatttctc
      781 aaacgaagca gaaaacgcaa ggaaattcca tgaaaacttc aaggactcaa aaaccgtaaa
      841 gatacctaag gtctacgact ccctttccac aaggaaagtc ctgattttgg aatttattga
      901 aggagtcgag cttcattacc tagacaagct aaaagaaaaa aaaggctaca acatcaaggc
      961 gataatgaaa aatggatttg atagcatcct cacccaggta tttatccacg gctttttcca
     1021 tgcagacccc cacccgggaa acatccttgt cttaaagaac aacaagatat ctttcatcga
     1081 ttttggcatc gtcggatatt ttgatgaatc gttaaagaag aaaagcatag agcttttcta
     1141 tggcatcatc gaggaagata tggacgcggt tgcagacgcc ttccttgata tggggatggt
     1201 agatgaagaa gcgacaaaca tagagtcctt caaaagagag ataaagttga tagttgacag
     1261 cttgcaaaag accaaactca aagatgttaa gataagctat gtgctggaaa aagtacttga
     1321 tgtttgcttc aagcataaag tgaagatgcc gattgacttt gtcctttttg gaaaagctat
     1381 agttgagttc gaaggcatcg gccttgaata tgagcctgac tttgtcttta gcgatgctgc
     1441 aaagcctttc ctggaaaagt tgataaagaa acagtttagt gttaagacaa tggcaaagga
     1501 gctgcagaag aacgctctaa gatatggtaa attcctaaga gacttgcctg accaggttag
     1561 ttctgtatta aagaaagtcc agaaagggac cattaagata gatgtccagg acacagatgt
     1621 aaaacagttg ggaagagaca tcgaccgttc aagtaacagg ctaacatatg gcctcatcat
     1681 tgcagcattc ctcatcacag gtgctctttt ggtggatgtc ggaaagcctg gcttctacgg
     1741 ctattcgatg atttcagtgc taagctttgg ccttgcctta atgtttcttg cactcttgat
     1801 tatttcaata ctaaatgagg tgaaacaatg aaacaagtta caaaaaaaag ccttttgaca
     1861 gcaataggcg cagcaagcat agcagctgag aatgcccaaa aaatacttaa ggatatctca
     1921 aagaaaggct tgataagtgc aaaggagaca agaaagatag tcagttctct tgtaaaagag
     1981 acagaaaaag agaggaaaaa gatagtgaag atagtctcaa aagagctaaa ggactcagca
     2041 aaaaaagcag agccttacct aagaaacaaa gtaaggaccg cagtaagcag cctcaaaaaa
     2101 gcccaaagga agatggaaaa caaaggcaaa gcagtggtaa agaagctaag ataaaatgcc
     2161 ttttgctgta actcacgtca tcctgactat agttgctttg gacataataa gggactacat
     2221 aataaaggac agaaaaacat ttccattgca ctatcttttc atcggcggag ttgcaggcct
     2281 gctgcctgac atcgatattc cacttttctg gctgcttgat gctctgggca tcagcgtatc
     2341 ctggtttcat cgcacgttca cccatagcct gctctttgca tttgccttca tgctggttac
     2401 agtaactctc ttcttatact caaagaaaag gaagcattgg cttcttgcgg cagtggccac
     2461 ttttggagtt agctttcatc ttttacttga cggtcttttg ggtggctaca tcatgctctt
     2521 ctatccattt tcaacaatag gctttggcat tgaccttgca caatatatta ctatgccggc
     2581 gatttggcag ggcatcgacg cattgatatt attggtatgg ctctgggatc tcgacagaag
     2641 gcacaatcta agagattata tgtaacattt tcaataaatt tatataccca caaatattat
     2701 tatttaccca caggtgatgc gtatgaaaac tgtatctgca gatgaaagag gaagaattac
     2761 tctaggaagc gacatgctgg atagatatgg caaaaaattt attgtgattg ctgcagaaaa
     2821 agagattgta ctcgtgccta ttgctgagga cccgcttggc gaattgaaaa agattggaaa
     2881 aagcgcaggt attgatagtt ttacctttaa ggaattgaag aggatggcaa aagaagaagc
     2941 ttcaaaagag gccctatcag ggtaatcatg tttgcagata ctgacttcat acttggactg
     3001 cttaaggact cagactggtt gaaggaaaat gcagtaaaga tcctcgatgg gtataaaggc
     3061 aagataaaga catccatttc tgttatgatt gaggttgcgc tgatatgcaa gcgcctaaat
     3121 atggatgtgt tgaatacctt tgcccatatc tttgagataa tcatcgttga tgaaaaaaca
     3181 caagaagtct gtcttcgggc agcgttgtat atcactaagt atgggttagg agtctttgac
     3241 agttttaatg cagcgtttag tatcaatgat aagataataa gctcagattc ggtctatgat
     3301 accctaggtt tggccaggtt gaatctaaga ggatgattat tctataaccg tccccttaaa
     3361 cttctttcca gaaataactt tctcaaggtt cttaaggtcc ttgcctacga tgactacttt
     3421 aagccccagt ttttcagcct ctcttgcagc tatcggatca aaaggcatgt ttaaacccgg
     3481 cttccatttg ctgccgacaa gctgcctgag tgccttccag gatatttttt cgataggctt
     3541 tgccccagaa atcttagggt ctttgtcata cacataatca acatttgtca tatttatcac
     3601 agtgcctgcc ttgatattct tggcaagcag aacagcatcg gtgtctgtcg agaaccctgg
     3661 cttccatcct gcagcgacaa gtatcctctc tttttcttct atcttcttgg tagggtttgt
     3721 gattatattg cctttaatac ctaatatctt cttgagcgtc agtgcattta tccttgtggc
     3781 atatatccct atccagtcaa gctcttttgg ggttatacca tttatctttc tggcatttgc
     3841 cataaggttt cttgcaagct ttcctccgcc gcagatgacg atgaactcct tgcccttatg
     3901 tttcttgata agttcaccaa gccttcttac aaaagagatg tcaaatttgt caggtatgat
     3961 tacagagccg cccaaagata agaccacttt ttccataatt tttattcctc taaagctatt
     4021 ataaaaacct ttctctcaag gtaattctta tctaataaga taaaatggca atatttataa
     4081 ataaattctt atctaataag aataaatggc cattgttcat ggcatggata aggaatcttc
     4141 ctctattctt gcctctatga agaaaaagca tgctcagata gaatcagaag tagagcatca
     4201 tttggagaaa aaggagcttt gtcttcctgt ctctattttt agcgaaaatc taggcatgct
     4261 tgagtcagct gtcatctatc taagagatac tatgggctta aggttcacag agatagcaaa
     4321 gctcgtaaag cgcgactata agacagtctg ggcgtcgtat acaaacggaa agataaagag
     4381 caaaagatga aagacttaaa aacctcactc atagagtata tcggagcatg tttgggtaaa
     4441 cagacttccc cgaagtagag cataaataat attaaggagt gtatcaatag attaagacgt
     4501 tgggaggtaa gtcccaacgc ttgtcgataa ccatgcaaat agatatcgac aaaattagga
     4561 aaactttctt taagaaaa
//
LOCUS       DASRUK010000025        13146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1196898, whole genome shotgun sequence.
ACCESSION   DASRUK010000025 DASRUK010000000
VERSION     DASRUK010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 13146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13146
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196898"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..284)
                     /locus_tag="VFF28_01075"
     CDS             complement(135..284)
                     /locus_tag="VFF28_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44253.1"
                     /translation="MGYRADYLTNIEELEQEIERHKSTESILEEKDTDNPQITLNFDR
                     AKNEL"
     gene            complement(256..879)
                     /locus_tag="VFF28_01080"
     CDS             complement(256..879)
                     /locus_tag="VFF28_01080"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZX44254.1"
                     /translation="MEIYQEELKIIDFFRNHLFSELTLRELMKEIGKKSYPWTYNAVA
                     KLGKDILITEKRGSTILAKLNLDSSTAITYLTFLDKKEAYQKKIPLVDKIIKSCSKLT
                     PFFILMVVGSYAQGKMRESSDVDLVIITEKKTEIKPYVTEITNLSGTDYDINIFTKDE
                     FYKMLVSLEENFGKEAFRKHLLFYGTEAYYEIIKEAAKNGLQSRLSY"
     gene            complement(956..1732)
                     /locus_tag="VFF28_01085"
     CDS             complement(956..1732)
                     /locus_tag="VFF28_01085"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44255.1"
                     /translation="MPNELYLIGTVHNDPDGKDRLCKLLNLSSPSLVAVESHKDCIAP
                     ELSVDDYLAIYREVTEEAGIHLTDRQATTFRQATKHEMEWHAFELQAAKEYTQSNPNS
                     KLRYIDEIIPGSQDQKNLLALQRCAVQQLVSDDEFVRLWLSDLDEGLEACLRRTREGI
                     DFVYTNYNLLTNYASEVRSFLEHTEDMPSALNEVAEIFKTAYSWERENRMGQRIRELY
                     PSSNRLVAIFGLAHLSGIMGYLSDLRPKSVLLNQYKPITG"
     gene            1923..2207
                     /gene="gatC"
                     /locus_tag="VFF28_01090"
     CDS             1923..2207
                     /gene="gatC"
                     /locus_tag="VFF28_01090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HZX44256.1"
                     /translation="MEIDSKLIKHIASLARLELTEKEIKQFEPEMEEILNAFSALDEV
                     DTKDTKPSFHPVELKNFMREDKTRPCLTQAEALANAKEKKDGYFKGPMAV"
     gene            2210..2731
                     /locus_tag="VFF28_01095"
     CDS             2210..2731
                     /locus_tag="VFF28_01095"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZX44257.1"
                     /translation="MKRIILALAIILIVGCKGVSNMKTAVFDTTKGTFEIQLYTDKAP
                     ITTQNFIDLTNKGFYNGQKFHRVIDGFMVQGGDPNGDGTGGPGYTIKDEFHPELRHDS
                     EGILSMANRGPNTGGSQFFITLAPTGWLDDKHAVFGKVIKGMDIVRSIGSSPTGPGDK
                     PLQDIIIKKITIK"
     gene            2733..4070
                     /locus_tag="VFF28_01100"
     CDS             2733..4070
                     /locus_tag="VFF28_01100"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HZX44258.1"
                     /translation="MEIKDIIAETEKINKEYHYFNAIDKEPLKGTGRLENIAVSIKDS
                     ICVRGLESRAGSGILEGYKPVFDATVVKKIKEAGGIIIGKTAQDAFGFGSFSTNVGKG
                     LKVPLNPIDKERTCGGSSGGAAGFTKKANFKHIAIGQSTGGSIVCPASFCGVVGLCPT
                     YGRVSRYGLMDYANSLDKIGPIAKTVEEAALMLEIIAGFDENESTTLDTPVPRYSDFL
                     DKDVSGLKIGIIKEAFGEGIDEEVKNKVENAIGCIEGQGAKVETVSLPLTQKYGLPAY
                     YLLAMCEASTNLARYCGMRYGAAEQVKGSFNEYFSKVRSAYFESESKRRIMLGTFTRM
                     AGYRDAYYIKAAKIRTKIIEEYKIAFKKFDLLASPTMPFVAPRFDEIKKLTPLQNFMA
                     DILTVGPNLAGLPHISIPIGVNSGKMPVGLMLIADHLKEETLIQAGSHLESVK"
     gene            4067..4654
                     /locus_tag="VFF28_01105"
     CDS             4067..4654
                     /locus_tag="VFF28_01105"
                     /inference="COORDINATES: protein motif:HMM:NF014886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HZX44259.1"
                     /translation="MTFKEKTIVYFDKPGKDNTDKALDLAVAYANTNSIKKIIIASAT
                     GKSALKLKEKAKDIQIVAVTYSEAVPYKEELEEFKKNEAELIKRGIKVVRCTHAFSGI
                     SKSISTKFKTNTPHVLIAETLKLISEGTKVAVEASLMAADVGAVKKEPVLALGGTEEG
                     IDTCLLVEPATTSDFFKFGILEIICIPRFGGLFHG"
     gene            4647..6059
                     /gene="gatB"
                     /locus_tag="VFF28_01110"
     CDS             4647..6059
                     /gene="gatB"
                     /locus_tag="VFF28_01110"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HZX44260.1"
                     /translation="MAEFTTDIVIGLEIHTELKTASKLFCGCPREGSDIPNSRTCPTC
                     LGMPGSKPVLNKKAVEFAIKLALASGSTISPDLVFSRKSYFYPDMAKNYQISQYEIPL
                     AIEGHIKLESGKKIGLTRIHMEEDPAALIHPSGMRDSKFVLVDYNRSGNPLVEIVTKP
                     ELTNPEEARDFMKQLITVLEYLEIHDDSCIIKADANISIKESGYIRSEIKNITGFKEI
                     ERALKYEVARQKKDHAEGKILIQETRAWDSEMGITFSLRKKETEADYGYIVDPDLVKI
                     ELNKDWIAEIKKHMPELAHEKLKKFTEEHGITEEMAKVISKDKQLAEMFEAVSTAVDP
                     ELAAKWIRRELARVLNFSKKRLSETEIKDSHMIDLLTLVQQKKITENVAQRLIEKLVE
                     APFDIREYVKKEGLEAVSDATELQKVCKEAIEEAPKAVEEYQKGNEKSLNFVVGIVMK
                     KTRGKASPKEVNEILRKSLK"
     gene            6060..6704
                     /locus_tag="VFF28_01115"
     CDS             6060..6704
                     /locus_tag="VFF28_01115"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HZX44261.1"
                     /translation="MKKAVVCWTGGKDSALALYEARLLGYEITQLVTFVPDEPNFLAH
                     PLHLIKLQSEAIGIPHKEMTIREPYKESYEEAIQSLKAEAAAIVTGDISEIAGHPNWI
                     KECSKKSKMEVLAPLWYQDREYLINRLLSKKFKVIFSLIKKPWFKDDSLLGKELDKNT
                     LLELRSICKNTGMDLCGEQGEYHTLALDGPLFNKPISVNFSKNSKDNISYLKLD"
     gene            complement(6696..7904)
                     /locus_tag="VFF28_01120"
     CDS             complement(6696..7904)
                     /locus_tag="VFF28_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HZX44262.1"
                     /translation="MKFAHIADCHIGGWREPKLRNIGVIAFERAIDTCIEKNVDFVVI
                     AGDLFNTSLPSIEHLKPVVAKLKGLKDKSIPLYIIAGSHDFSPSGKTMLDVLENAGLV
                     INVARGEEVDGKLRLLFTVDPKTGVKITGLLGKKGGLEKNYYECLMKDELECEKGEKI
                     FLFHSLLSELKPKELEHCDSQALSLLPKNFSYYAGGHPHFVKDVSIEGYGKIAYPGPL
                     FPNNFKELEELSCGGFYIIDDGRIKFEQIQVFNVFAIKILSDHRTPLDITNEILEKIR
                     GQAFNDTIVTIRVSGSLESGKTSDVDFKTIFDRLYEKSAYFVMKNTNGLIAREFEEVK
                     IDARDVEDVEEKLISENIGQVKVEGMNDESAMTKHLMKAFSMAKDEGEKVFDFERRIK
                     DEAGKILELI"
     gene            complement(7901..9421)
                     /locus_tag="VFF28_01125"
     CDS             complement(7901..9421)
                     /locus_tag="VFF28_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008081976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZX44263.1"
                     /translation="MVKGQVISGEFGKILIRQKAGERIELGELLVGESSDSKILLQAV
                     NLLYGSQISQQNLELISGINLEEGQDASFMEPELRNYNLAVLKNLITLNGDNASVCKK
                     LPAFFSSVREIRKEDLSFLVKPKNPLFIGNLRSGSKMMDFPIYLDGADVFSHHVLVPA
                     TTGRGKSNMTSVILWDALSKDYCGILVLDPHDEYFGRNKLGLKDHPMKEKVVYYTPNE
                     VPVGQRTLKINLSLVKPHHFSGVVDWSDAQLQALTQYFREFGNDWIEAIVLDKPLKAD
                     FFEGTLAVVKRRIMSLLDLEFNGSQLFSNGVFDLNAGETTITDICHELEEGKAVIIDT
                     SDFSGSVEILIGSLIAHEILGNYRRFKMKGTLREKPVVSIVLEEAPRVLGKEALERGP
                     NIFSTIAREGRKFKVGLFAITQLPSLIPREILANMNTKIILGIEMKPEREAIIQSAAQ
                     DLSEDDRNIASLDKGEAIISSNFAKFATPVKIPLFEDFAKLSKKEEVKRDFGGLIR"
     gene            9629..10795
                     /locus_tag="VFF28_01130"
     CDS             9629..10795
                     /locus_tag="VFF28_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44264.1"
                     /translation="MPGNPRRFLLYSALTLSLASPIPAQEKPTKDQIKASQILNDSRD
                     EGSLERMIEQEYGFNLRIDSPYDPNMLRRFYNIVGEIHRCNPALVASARGLDIHLLPG
                     WKDALYVATCTAGKEMEYYKDSMTDAGTVVHEWAHSQLTRKKDFWDKWCEIVSRHGFQ
                     YNSNLTFDHQWVNWTDYPYEPQGVFCRPYASKNKHEHAAEIRRYIYENRPFQIDSREA
                     FDCYKDLLRHCLNSKVFSEIEYAQGIKLLSPAEIIKPDTDIEKLAGRWKTPWCKIAEA
                     NQLWDIKKIEGPYKAFIPIIEINDAAKKEPDYLLHQARPNEKLQNILVHYKPLYPYLT
                     EKMLIDNNPKIVDPDKLREFEIVNIPLIRNGASWYREIPSMASFAGKAKVNYGY"
     gene            complement(10779..11471)
                     /locus_tag="VFF28_01135"
     CDS             complement(10779..11471)
                     /locus_tag="VFF28_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44265.1"
                     /translation="MKAIVFDSGPVISLTVNNLLGLLTSLKERYNGCFSITDSVRKEL
                     IERPLETKKFKFEALQVLRCIKFDVLTVHESPRDLTIKLLDLANKAYIAKGNCVQVVH
                     YAEMSSLAAAILNEADAFVVDERNTRLLLEEPERLAEILSRRLHTEIKVNKKNLDELR
                     RLAKGIRLIRSVELITVSFEMGLLDKYVVDIPNARKTLLEAVLWGVKLNGCSVSEEEI
                     DEILKIEINSRN"
     gene            complement(11468..12019)
                     /locus_tag="VFF28_01140"
     CDS             complement(11468..12019)
                     /locus_tag="VFF28_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44266.1"
                     /translation="MDENVRNDIISVLNDAADILTAEQERDAADLIELSNHTIHNASI
                     FQDEDSISVAIFIYALSKVIERREGQLNYGFIRKMIIAARDDIKSERADLFRNSMKKA
                     FDFISSLDSKLKLYVEEVIKQAEIRKGSKLYAHGISLARTAEILGVSQWELMAYVGNT
                     RLTDSAGGLSVKQRILYARSLFR"
     gene            12451..12924
                     /locus_tag="VFF28_01145"
     CDS             12451..12924
                     /locus_tag="VFF28_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44267.1"
                     /translation="MKISIRKGFSFGLASGIITTLGLIVGLHSGTNSYIAVIGGILVI
                     AIADAMSDALGIHVSEEFENRHSQLEIWEATIATFLFKFIFSLTFIVPILLFELSAAI
                     IVSVIWGLSLIIVFSYFMAKQQKVKPFGVVFEHLLIAIVVVILTHYIGEWVGTLG"
BASE COUNT         3987 a         2811 c         2660 g         3688 t
ORIGIN      
        1 tgttggcaat tggttttatc ctactattcc tgaaatggaa agggcaatta tgaaattctt
       61 cagaagtctt tggtacaaca aacaaaaagt tctatctctt attccattta atgagaaata
      121 ttcaatttaa tttactataa ttcatttttt gctctgtcaa agtttagcgt tatctgggga
      181 ttgtcggtgt ctttttcctc aagaatgctt tctgtactct tgtgcctctc tatctcttgc
      241 tccaattcct ctatattagt aagataatct gctctgtaac ccattttttg cagcctcctt
      301 gataatttca taataggctt ctgtcccata aaacaaaagg tgttttctaa aagcttcctt
      361 tccgaaattt tcctctaaag agacaagcat tttgtaaaat tcatcttttg taaagatatt
      421 tatatcataa tcggttccag aaagatttgt aatctcggtc acatacggtt ttatctctgt
      481 ctttttttcg gttattatga ctagatcgac atcagagctt tctctcattt tgccctgggc
      541 atagctgcct acgaccatta gaataaaaaa aggggttagc tttgagcagc ttttgatgat
      601 tttatcgacc aaaggaattt ttttttggta agcttctttt ttatcgagaa atgtaagata
      661 ggttattgca gtgctgctgt caagattaag tttggcaagg atagtgctgc cgcgtttttc
      721 tgttatcaat atatcttttc caagcttggc tacagcattg taggtccagg ggtaagattt
      781 cttgccgatt tctttcatca gctctctcaa tgttagttct gaaaaaaggt ggtttctaaa
      841 aaaatcgatt atttttagct cctcttggta aatttccatt ttatactacc tgaagtatag
      901 atttatactg tacaaagtat aagttatatt taagagtggt ggtttttctt tgggattacc
      961 cagttatagg tttatattgg ttaagtaaaa ctgattttgg ccttagatca gaaagatagc
     1021 ccataattcc tgaaagatga gccaatccaa aaattgctac aagacgatta ctactaggat
     1081 atagctctct tattctctga cccattctgt tttccctctc ccaagagtaa gctgttttaa
     1141 agatctctgc tacttcattt aaggcagagg gcatgtcctc tgtatgttct agaaatgacc
     1201 tgacctctga tgcatagtta gtaagcaagt tatagttggt gtaaacaaag tcaattcctt
     1261 ccctagtcct tctaagacat gcttctagac cttcatccaa atcagataac cacaatctga
     1321 caaattcatc atctgaaacc aattgctgga cagcgcacct ttgcagagcc aataaatttt
     1381 tttgatcttg gctgccaggt ataatctcat caatatatcg taatttagaa ttgggattgc
     1441 tttgggtgta ttccttagca gcttgcaatt caaaggcatg ccattccatt tcatgtttag
     1501 ttgcttgtct aaaagtagtt gcctggcggt ccgtaagatg tatccctgct tcttcggtta
     1561 cttccctgta gatcgctaga taatcatcaa ctgacaattc gggtgctata caatccttat
     1621 gagactcaac agccactaga gagggagatg atagatttaa tagtttgcaa agtctgtctt
     1681 taccatcagg atcattatgt actgttccta tcaaatatag ttcattaggc atatgccgag
     1741 gaaggctggt ttaattatat ataccccaac ctcaaattct tattatttag ttccagccat
     1801 ttacctccta taatatcaat cataataatg ggtgtttaaa tcttgtgatt tataactact
     1861 aaatagtaat gactgatttt atttagcaaa ttatataaac cctccaaact cccaatcatc
     1921 caatggaaat tgacagcaaa ctgatcaagc acatcgcctc attggcaagg cttgagctga
     1981 ctgaaaaaga gataaagcag ttcgagccgg agatggaaga gatcctaaac gccttctcag
     2041 cccttgacga ggtcgacaca aaagatacaa agccttcttt tcaccctgtt gagttgaaaa
     2101 actttatgag ggaagataag accaggcctt gccttactca ggcagaggct ttggcaaacg
     2161 caaaggaaaa aaaagacggc tacttcaaag gccctatggc ggtgtagata tgaaaaggat
     2221 aattttggca ttggcaatta tattgatagt tggctgcaaa ggagtaagca atatgaaaac
     2281 agcagtattt gatacaacaa aaggaacatt tgagattcaa ttgtatacag acaaagcacc
     2341 cattacaacc caaaacttca tcgatcttac aaacaaagga ttctacaatg gccaaaaatt
     2401 ccatagggtt atcgacggat ttatggtgca aggcggcgat ccaaacggag acggaacagg
     2461 cggcccagga tacacaatta aagacgagtt ccatccagag cttaggcacg actcagaagg
     2521 catcttatcc atggcaaaca gaggcccaaa cacaggcggc tcacaattct tcataacatt
     2581 agctccgaca ggatggctcg atgataaaca cgcagtcttt ggcaaagtca taaaaggcat
     2641 ggacatagta agatctatag gcagctcgcc aacagggcca ggggacaagc ctctgcagga
     2701 catcataata aagaagataa ctataaagtg atatggaaat taaggatata atagctgaga
     2761 cagagaagat taacaaggaa taccactact tcaacgcaat agataaagag ccgcttaaag
     2821 gcacaggcag gcttgaaaac attgcagtat cgataaagga ctctatctgc gtcagaggcc
     2881 ttgaatcaag agcaggctct ggtatccttg aaggctataa gccagtcttt gatgcgacag
     2941 tcgtaaaaaa gataaaagaa gcaggcggaa taattatagg aaagaccgct caggatgctt
     3001 ttggatttgg ctcattctca actaacgttg gaaaagggct gaaagtcccg cttaacccta
     3061 tagacaaaga aaggacatgc ggcggctcat caggcggcgc agcaggtttt acaaaaaaag
     3121 caaacttcaa gcatattgca ataggtcaaa gcacaggcgg ctctattgtc tgcccagcat
     3181 ccttctgtgg cgtagtaggc ctttgcccga catatggcag agtctcaagg tatggtctta
     3241 tggactatgc aaacagcctt gacaagatag gccctattgc aaaaacagtt gaagaagcag
     3301 ccctgatgct agaaattatc gccggatttg atgaaaacga aagcacgacg ctagacacgc
     3361 ctgtcccaag atactctgat tttctggata aagatgttag cggtctcaag attggtataa
     3421 tcaaggaagc atttggcgaa ggcatcgatg aagaggtaaa aaacaaggtt gaaaatgcta
     3481 taggctgcat cgaaggccag ggagcaaaag tcgaaacagt atcccttccg ctcacgcaaa
     3541 agtatggcct tcctgcgtat taccttttgg ccatgtgcga agcatccacc aatcttgcaa
     3601 ggtattgcgg catgagatac ggcgcagcag agcaagtcaa aggaagcttt aatgaatatt
     3661 tctcgaaagt taggagcgct tattttgaaa gcgaaagcaa aagaaggata atgcttggaa
     3721 catttacaag gatggcaggc tatagggacg cttattacat aaaggcagca aaaatcagga
     3781 caaagattat tgaagagtac aaaatagctt tcaaaaaatt cgaccttctc gcaagcccga
     3841 cgatgccttt tgtggcacca agatttgacg agataaaaaa gctcacgcct cttcaaaatt
     3901 tcatggcaga tatccttaca gtaggcccaa atcttgcagg ccttccccat atcagtattc
     3961 caattggcgt aaacagcggc aagatgcctg tcggccttat gcttattgca gaccatctaa
     4021 aggaagagac tctcatccag gcaggctctc atctggagag tgtaaaatga cattcaaaga
     4081 aaaaaccata gtctactttg acaagccagg caaagataac acagacaaag ctttggactt
     4141 ggcagtagca tatgcaaata ctaacagcat aaaaaagatt atcattgcat cagccacagg
     4201 aaagtcagcc ttaaaactaa aagaaaaagc aaaagacatt caaattgttg cagtcaccta
     4261 ttcagaagct gttccatata aagaagagct tgaagagttc aagaaaaacg aggctgagct
     4321 gattaaaaga gggataaaag ttgtaagatg cactcacgcc tttagcggaa tcagcaaatc
     4381 catctcgacc aagtttaaga caaatactcc ccatgttctt atcgcagaaa cgctaaagct
     4441 tatctcagaa ggaacaaagg tagctgtgga agcatctctc atggcagcag acgttggagc
     4501 agtaaaaaaa gagccagttc ttgccttagg cggaacagaa gaaggcatcg acacatgcct
     4561 cctggtcgag cctgcaacaa cctctgattt tttcaagttt ggaattttgg agataatctg
     4621 catcccaagg tttggaggtc tgttccatgg ctgagtttac aacagatata gttatcggcc
     4681 tggagatcca tacagaacta aagaccgctt caaagctgtt ctgtgggtgt ccaagggagg
     4741 gtagtgatat acctaactca cgcacttgtc caacttgttt aggaatgcca ggcagtaaac
     4801 cagtcctcaa caaaaaagca gtcgagtttg caataaagct tgcactagcc tcagggtcaa
     4861 caatatcacc tgaccttgtc ttctcaagaa aatcatattt ctatccggac atggcaaaaa
     4921 attatcagat ctcgcagtat gaaattccgc tcgcaataga aggccacatc aagcttgagt
     4981 caggaaaaaa gataggcctt acaaggattc atatggaaga agacccggca gctttgatcc
     5041 atccttcagg catgagagat tcaaagtttg tcttggttga ctacaatagg agcggaaatc
     5101 cattggtgga aatagttaca aagccagagc tgaccaaccc agaggaagca agggatttca
     5161 tgaaacagct catcaccgtt ctagaatacc ttgaaatcca tgatgattca tgcataatca
     5221 aagcagatgc aaacatctct attaaagaaa gcggctatat ccgatcagag ataaaaaaca
     5281 taaccggctt taaagagata gagcgggcat taaagtatga agtcgcaagg caaaagaaag
     5341 accatgcaga aggaaaaatt ctcatccagg agacaagagc ctgggactca gagatgggca
     5401 tcacgttctc actaagaaag aaagagacag aggcagacta cggctatatc gtcgatcctg
     5461 accttgtaaa gattgagcta aacaaagact ggatagctga gataaaaaag catatgcccg
     5521 agcttgccca tgaaaagctt aagaaattta ccgaggagca tggcataaca gaagagatgg
     5581 caaaagttat ctcaaaggac aagcagcttg cagagatgtt tgaagcagtg agtacagcag
     5641 tagatccaga gcttgctgca aaatggataa gacgagagct tgcaagggtg ttgaacttta
     5701 gcaaaaagag gctaagcgag actgaaataa aagactcaca tatgattgac ctgttgaccc
     5761 ttgttcagca aaagaagata acagaaaacg tagcccaaag gcttatagaa aaacttgttg
     5821 aagctccctt tgacatcagg gaatacgtaa aaaaagaagg ccttgaagca gtctctgacg
     5881 ctacagagct tcaaaaggta tgtaaagaag cgatagaaga agcgccaaag gcagttgaag
     5941 aatatcagaa aggaaacgag aaatcattaa attttgtcgt aggtatagtc atgaaaaaga
     6001 caaggggaaa agcaagccca aaagaagtga acgagattct aaggaaatca ttgaaataaa
     6061 tgaaaaaagc tgttgtctgc tggacaggtg gaaaagactc tgccctcgcc ttatacgaag
     6121 caagattgct aggttatgag ataactcagt tagtcacttt tgtcccagac gagccaaact
     6181 ttcttgcaca tcctttgcac cttattaaac tgcagtcaga agctataggt atacctcata
     6241 aagagatgac gataagagag ccttacaaag aaagctatga ggaagcgata cagtcattaa
     6301 aggcagaagc agccgccatt gttactggag atatatcaga gatagcaggc catcccaact
     6361 ggataaaaga atgcagcaaa aagtcaaaga tggaagtgct tgcaccccta tggtatcaag
     6421 acagggaata tctgataaac cgtttgctat ccaagaaatt taaggtcatt ttctctttga
     6481 taaaaaagcc ttggtttaaa gacgatagcc tgcttggaaa agagctggat aaaaacacac
     6541 ttctggagct tcgttcgatc tgcaagaaca caggtatgga tctatgtggc gagcaaggcg
     6601 aatatcacac gctggcttta gacggcccgc tctttaataa gcctatatcg gttaactttt
     6661 caaagaattc taaagataac atctcttact taaagttaga ttagctctaa tatcttccct
     6721 gcttcatcct ttatccttct ctcaaagtca aagacctttt ctccctcatc ctttgccata
     6781 gaaaacgctt tcatcaggtg ttttgtcata gctgattcat cattcatccc ttcgaccttt
     6841 acctggccta tattttcaga gataagtttc tcctctacat cttcaacgtc ccttgcatcg
     6901 attttcactt cctcaaattc cctggcgata aggccattgg tgttcttcat cacaaaataa
     6961 gctgactttt catacagcct gtcaaatata gtcttgaaat ctacatccga tgtttttcca
     7021 ctttcaaggc tgcctgacac cctgatagtg actatagtgt cgttgaatgc ctgccccctt
     7081 attttctcaa ggatttcgtt ggttatgtcc aggggtgtcc tgtggtctga taagatcttt
     7141 atcgcaaaga cattaaaaac ctgtatctgc tcaaacttaa tcctgccgtc gtcgatgatg
     7201 taaaagcctc cgcacgaaag ctcttctagc tccttaaagt tgttaggaaa taatggtccc
     7261 ggataagcta tctttccata tccttcgata gaaacatctt tgacaaagtg aggatgccct
     7321 cctgcataat agctaaagtt ctttggaagc aggctcaaag cctggctgtc acagtgctca
     7381 agctcttttg gcttaagctc cgaaagcagc gaatggaaaa gaaatatctt ctctcccttc
     7441 tcgcattcaa gctcatcctt catcaagcac tcgtaataat ttttctcaag gccccccttt
     7501 ttgccaagaa gccctgtaat cttcacccct gtcttaggat cgacagtaaa gagcagtcta
     7561 agtttgccat caacctcttc ccctcttgcc acgttgatga caagccctgc gttttcaagc
     7621 acatcaagca tcgtctttcc agacggagaa aaatcatgcg agcctgcgat gatgtaaaga
     7681 gggatagact tgtctttaag ccctttcagc ttggcgacca caggctttaa gtgctcgata
     7741 gaaggaagcg aggtgttaaa caagtcgccc gcaataacga caaagtcaac attcttttct
     7801 atacatgtat cgattgccct ctcaaaagct atcactccga tatttctaag ttttggctcg
     7861 cgccagccgc ctatatggca gtcagcgatg tgggcaaact tcaccttata agccctccaa
     7921 agtctctttt gacctcttct tttttggaaa gctttgcaaa atcctcaaac aacggaattt
     7981 ttacaggtgt tgcaaacttt gcaaaattag aactgattat agcctctccc ttgtcaagcg
     8041 acgctatatt tctatcatct tcagaaaggt cttgtgcagc gctttggatg atagcttccc
     8101 tttcaggttt catctcgatt cctaggatta tctttgtatt catattcgcc aatatctcac
     8161 ggggaataag cgaaggcagc tgggttatgg caaacaaccc gactttgaac tttcttcctt
     8221 cacgagcgat ggttgaaaat atatttggcc ctctctcaag cgcttctttc ccaagcactc
     8281 ttggagcttc ttcaagcact atagatacta ccggtttttc cctaagggtg cctttcatct
     8341 taaaccttct ataattaccc agtatctcat gcgcaattag gctcccgata agtatctcca
     8401 cgcttccaga aaaatcagac gtatctatta tcactgcttt cccttcttca agctcatggc
     8461 atatgtctgt aatcgttgtc tctcctgcat taaggtcaaa gacgccatta gaaaacagct
     8521 gcgagccatt gaattcaaga tcaaggaggc tcattatcct tcgtttgaca acagcaagcg
     8581 tcccttcaaa aaagtcagcc ttcaaaggct tgtccaagac tatcgcttct atccaatcat
     8641 ttccaaactc cctgaaatat tgcgtaagtg cctgcaattg agcatcgctc cagtcaacaa
     8701 cacctgaaaa atgatgaggc ttgactaaag aaaggtttat cttaagcgtc ctctggccga
     8761 caggaacctc gtttggagtg taataaacca ctttttcctt catcggatgg tctttgagcc
     8821 caagcttgtt ccttccaaag tattcatcat gaggatcaag caccagtatt ccgcaataat
     8881 ctttgcttag tgcatcccat aggatgactg aggtcatgtt gctcttgcct ctgccagttg
     8941 tggcaggaac aagaacatga tgggagaata catcagcgcc gtcaagatat atgggaaaat
     9001 ccatcatctt gcttccgctt ctaaggtttc cgataaagag agggttcttc ggctttacca
     9061 aaaaagacag atcttctttc cgaatctccc ttaccgagga gaaaaaagca ggcagttttt
     9121 tacagacact tgcattatca ccgttaagtg tgataagatt tttcagcaca gcaaggttat
     9181 agttcctaag ctcaggctcc atgaaagaag catcctgccc ttcctcaagg tttattcctg
     9241 agataagttc aaggttttgt tgagatatct ggctgccata aagcaggttt accgcctgaa
     9301 gcagtatctt gctgtcagat gattctccga ccaaaagctc gccaagctct atcctctcgc
     9361 ctgctttctg ccttatcagg atcttgccaa actctccaga tattacctgc ccctttacca
     9421 catggaaaag taatggctga gtttatttaa ggttttctag aaagttgatt gctcaaactg
     9481 atgctccgag agcttgctgc gatatctcaa tttgagatca aaaaaatacc ccgcagcttg
     9541 gccagcggtt tcggattttt tttaaaacaa tttccttaat agttaaagat agcattttta
     9601 tccctagctt ctgctaaaca tctaccggat gcctggtaac cctagaaggt ttctgttgta
     9661 ttctgcctta actttaagtc tagctagtcc tattccagcc caggaaaaac ctacaaaaga
     9721 ccagataaaa gcgtcccaga ttctaaatga ctccagagac gaaggttctc ttgagcgcat
     9781 gatagagcag gaatatggat ttaacctaag gatagattct ccttacgatc caaatatgct
     9841 aaggcgtttt tataatattg taggagagat acatagatgc aaccctgcac ttgtagcttc
     9901 tgcaagaggg cttgacatcc accttctgcc aggctggaaa gatgcccttt atgtggcaac
     9961 ctgcacagca ggaaaagaga tggagtatta caaggattcg atgacagacg ctggcacagt
    10021 tgtccatgaa tgggcccaca gccagttgac aagaaaaaag gatttctggg ataagtggtg
    10081 tgagatagta agccgtcatg gcttccagta caactcaaac cttacatttg atcatcaatg
    10141 ggtaaattgg actgactatc catatgagcc gcagggtgtc ttttgcaggc cttatgccag
    10201 caaaaataaa catgaacacg cagctgagat aaggcgctat atatatgaaa acaggccgtt
    10261 tcagatagat agcagagaag cttttgactg ctataaagat cttctaaggc attgcctaaa
    10321 ctccaaggta ttcagtgaga tagagtatgc tcagggcata aagcttctta gtccggcgga
    10381 aattatcaaa ccagacactg acatcgagaa gttagctgga aggtggaaaa ctccatggtg
    10441 taagatagca gaagcaaacc agctttggga tataaaaaag atcgaaggtc cctacaaagc
    10501 atttattccc ataatagaga taaacgacgc ggcaaaaaag gagcctgact atcttcttca
    10561 ccaggcaagg cccaacgaga aattgcaaaa tatattagtg cattataagc cattatatcc
    10621 ctatctcact gaaaagatgt tgatagataa caacccaaag atagttgacc cagacaagct
    10681 aagagagttt gagattgtca atattccctt gataagaaac ggtgcctctt ggtatagaga
    10741 gataccgtca atggcatctt ttgcaggcaa ggcaaaagtt aattacggct attgatctct
    10801 atcttaagta tttcatcgat ctcttcctca gaaacagagc agccgttaag ctttactccc
    10861 caaagcacag cctcaagcaa agtctttctt gcattgggta tgtcaacaac atatttgtca
    10921 agaagtccca tctcaaagga tacagtgata agctcgactg atcttataag ccttattccc
    10981 tttgctaatc tcctcagctc atcaaggttt ttcttgttta cctttatctc tgtatgaagc
    11041 cttcgtgaaa gtatctccgc aagtctttcc ggctcttcaa gtaaaagccg tgtgttcctt
    11101 tcatcaacta caaaagcatc tgcctcatta aggattgccg cagcaaggct gctcatctca
    11161 gcataatgca ccacttggac gcagtttcct tttgcgatat acgctttgtt tgcaaggtct
    11221 aaaagtttta ttgtcaggtc tcttgggctt tcatgcactg tcaggacatc gaatttaatg
    11281 catcttaata cctgcaaagc ctcaaatttg aatttctttg tctcaagcgg cctctctatt
    11341 agctcttttc tgacactgtc agttatgctg aagcatccat tatatctttc cttaaggctt
    11401 gtcaaaaggc caagaaggtt gtttacagta aggcttatca ccggccctga atcaaagact
    11461 atagctttca tcgaaaaagg ctccttgcat aaagaatcct ttgtttgact gaaagtccgc
    11521 cggcagaatc ggtaagcctt gtgtttccga cataggccat aagctcccac tggcttaccc
    11581 ccagtatctc agcagtcctt gcaagcgaga ttccgtgtgc atataatttg ctgcctttcc
    11641 ttatctctgc ctgttttatc acttcctcaa cataaagctt tagcttactg tcgaggcttg
    11701 agataaaatc aaaagctttc ttcatgctgt tcctaaaaag gtcagctctt tcggatttga
    11761 tgtcatccct tgctgcaatt atcatctttc tgataaagcc gtagttcagc tgcccttccc
    11821 gcctctcgat gaccttgctc agggcatata tgaatatggc gacgcttatg ctatcttcat
    11881 cctggaaaat agaagcgtta tgtattgtat ggttagaaag ctctatcagg tcggcggcat
    11941 ccctctcttg ttcggctgta agtatgtcgg cagcatcgtt aaggacgctg atgatatcgt
    12001 tcctgacatt ttcatccata actaagtttt agataaaaga gttatttaaa ggttgctgtc
    12061 aaacctatcc atttctctgt gaataatagc ttgggcaata ttgttcaaga tatctgttca
    12121 tagccgctct gtatttatcc atgaggtgtc gggttacctg tgcttgggat ccaatgattt
    12181 ctataagagc gtttttctcc ccacttgcca cctacatgat tcgttttagt aatccactcc
    12241 tgtattctat tcatctcttg gctaatcgtc tccgttaatt cctgcatctg tccagattgc
    12301 ccagccgcaa gagagagata ttgtggattt tgcgggagcc tctggcaaaa ttcctttgat
    12361 ggtctcattg acttaaaaga ataaaggctg ctttttaaat tatctttgaa gcaaaaaagt
    12421 ttttaaatca ggcctctact tgttcatgtg atgaaaatct caatccgtaa aggcttcagc
    12481 tttggccttg cctcaggcat aatcacgaca ttaggcttga tcgtaggcct gcactcaggc
    12541 accaattcct atattgcagt gattggtgga atccttgtca tcgctatcgc tgacgccatg
    12601 tccgatgcat tagggattca tgtttctgaa gagtttgaaa acaggcactc gcagttagag
    12661 atatgggaag caaccattgc aacattcctc tttaagttca tcttttctct tacgtttata
    12721 gtccctatcc ttttattcga attgtcggca gcgattattg tcagcgtgat atggggctta
    12781 agcctgataa tagtgttcag ctatttcatg gcaaagcagc agaaagtaaa gccttttggc
    12841 gtggtctttg agcacctgct gattgcaatt gttgtagtga tcctcactca ttatattggt
    12901 gaatgggtag gaactctggg atagcgcctg atttttttag aaaacacaaa atatatatac
    12961 taacccccaa agaatttaac attaagttaa aggggaatct atggtagtaa atatcattaa
    13021 aacatttatc aacaaagtct taaagaatat tttcaggaaa aaacagcaga taaagcttgg
    13081 gctctatggc cctccaaacg gcgggaagac cacgctggca aacaggatct gccaggactg
    13141 gcttgg
//
LOCUS       DASRUK010000026         3910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_693475, whole genome shotgun sequence.
ACCESSION   DASRUK010000026 DASRUK010000000
VERSION     DASRUK010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693475"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..1067
                     /locus_tag="VFF28_01150"
     CDS             174..1067
                     /locus_tag="VFF28_01150"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF018279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presenilin family intramembrane aspartyl
                     protease"
                     /protein_id="HZX44268.1"
                     /translation="MKHTLKVTIILVTLFFVAQVMGLLVTNAYIDHKATAESGEPVFI
                     SLPYEIERPPVEEAGSYVFILSSILIGTVLVLVLVRFRKVFLWKIWFFAAVVISLSIA
                     FSAFINPTIAFLISLGLAWLKIFRPSSIIHNVTEIFIYGGLAAIFVPIMSVTAAAILL
                     IIISAYDFIAVFKIKHMITMAKFQTESKVFAGLMLPYKKGKYNAKASKRPGKSKMRTA
                     ILGGGDIGFPLLFSGVVMKNLMLTDIVLVGFLKTLVITAAVSAALLFLFIKGKQDKFY
                     PAMPYLTAGCFLGYAIIFALL"
     gene            complement(1048..1959)
                     /locus_tag="VFF28_01155"
     CDS             complement(1048..1959)
                     /locus_tag="VFF28_01155"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZX44269.1"
                     /translation="MKASLTFLGTGQGSLVIGKNHLSSGGIVLQVDENQFWLDPGPNA
                     LKNAAENNINIRATTAILVSHAHLGHSNDTNAIIDAMTYSGFDKKGVLIASKSVINGS
                     NDFPPVLLPYSKTLLERYIAMEAGQMVGINDIEIKALKTKHSDQDAIGFKFFTSNFIL
                     AYSSDTKFAPELIDEYKGSNILILNVPSIKDPKANLCIEDAIKIIKEVKPKLAIITHF
                     GTDMVKGDPLSLTRNIQMAAGVQVITAKDSMVINPLSYSVSQRIIPSKQEEKKQELPE
                     VKPSDEAEQKVLESDILLKDIIKEEQK"
     gene            2097..2370
                     /gene="ffs"
                     /locus_tag="VFF28_01160"
     ncRNA           2097..2370
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFF28_01160"
                     /product="signal recognition particle sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01857"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal-type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01857"
     gene            2424..3011
                     /locus_tag="VFF28_01165"
     CDS             2424..3011
                     /locus_tag="VFF28_01165"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HZX44270.1"
                     /translation="MEFEVETTSRNVVKRLGLDYNFVLRAYKESGRVWDLYIGKISLD
                     ESWRAFYSALGVSMSYARFKDMRKEAFSNAPIDQEMTKIARKIKAQGYFLGIITNNTK
                     ERMNAISKNYGFDGLFDYMITSEDVMALKTDKKPFYEGLRRADVTANEVLFIDNSMSN
                     CEAAKRIGIQALFFDHKQRNYHRLYGELMKIGVKI"
     gene            complement(3008..3211)
                     /locus_tag="VFF28_01170"
     CDS             complement(3008..3211)
                     /locus_tag="VFF28_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44271.1"
                     /translation="MKLPLFIANAVGAVLLFNFLINPSITGFAVAEDTGFGLASPLGF
                     SIAFIVVVIALDVYSYVRSRQKA"
     gene            complement(3214..>3910)
                     /locus_tag="VFF28_01175"
     CDS             complement(3214..>3910)
                     /locus_tag="VFF28_01175"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HZX44272.1"
                     /translation="KIPFTKEQMTEAIKETVRKNKLKAGYIRPLCFFGYGKMGLNPEG
                     APVNCIIAVWPWGKYLGEEAVRVKTSDYVRIHPKSTDSEAKICGHYVNSIFASVEVKD
                     KGFDEALLLDYEGNVAEGPGENIFMVKDGKLLTPALGGILKGITRDSVIKIARDLGMT
                     VEEKTLKKEDIYSADEAYFTGTAAEITAIKSLDNNNVGKHAPGPITLKLRDAFMDIVQ
                     GKNEKYKDWLAYV"
BASE COUNT         1035 a          745 c          833 g         1297 t
ORIGIN      
        1 ttccttgctt ggtatgttgt ccatccttca ttcttacgcc taatagtctc cataagcttc
       61 cctctagtga gtttcatact ccaaagaaac tcaccgtagg gaaatagttt cgggataaca
      121 catgagaaat aatcttcatt aactttaaat accactgagt cctagaccaa gcaatgaagc
      181 atacgctaaa ggtgacaata attcttgtca ccctattctt tgtagcacag gtgatggggc
      241 ttcttgtgac caatgcttat atcgaccaca aggcgactgc tgaatcaggc gagcctgttt
      301 ttatcagctt gccgtatgag atcgaaaggc cgcctgttga ggaagctggc tcttatgtat
      361 ttatactcag ttcaatactg atagggacag tacttgtgct tgtcctggta aggtttcgca
      421 aggtttttct ttggaagata tggttttttg ctgctgttgt catttcgcta tcgatagctt
      481 tttctgcttt tatcaatcca acaatcgctt ttcttatcag ccttgggctt gcatggctaa
      541 agatatttag gccaagttca ataatccata atgtgactga gatatttatc tacggcggtc
      601 ttgctgcgat atttgttccc ataatgagcg tcactgctgc tgccatatta ttgatcatca
      661 tatctgccta tgattttatc gctgtcttta agataaagca tatgataact atggcaaagt
      721 tccagacaga gtcaaaggtt tttgcagggc tgatgcttcc ttataaaaag ggaaagtata
      781 atgccaaagc cagcaaaaga ccggggaaat ccaagatgag gactgctata cttggcggag
      841 gagatatagg atttcctttg ctattttcag gggttgtcat gaaaaatttg atgcttactg
      901 atatcgtgct tgttggattt ctcaagactt tggtgataac tgctgctgtc agcgctgctt
      961 tgctcttttt gtttatcaaa ggaaaacagg ataagtttta tcctgcgatg ccctatctta
     1021 ctgcaggatg ctttttaggc tatgccatta tttttgctct tctttaatga tgtcttttag
     1081 aagaatatca ctttcaagga ccttttgctc tgcctcgtcg cttggcttta cttcaggcaa
     1141 ttcttgtttt ttttcttctt gcttggaagg tatgatacgc tggcttactg aataagaaag
     1201 tggatttatc accatgctgt cttttgcggt tatcacctga actcctgcag ccatctgtat
     1261 gttccttgta agggagaggg gatcgccttt gaccatgtct gtaccaaaat gggtgataat
     1321 ggcaagcttt ggctttacct cttttattat ctttatcgca tcttcgatgc agagatttgc
     1381 ttttgggtct tttatgcttg gcacgttgag gataaggatg tttgagccct tgtattcatc
     1441 tatcagctct ggagcaaatt tggtgtctga agagtatgct agtatgaaat ttgatgtaaa
     1501 gaacttaaag cctatcgcat cttggtctga atgctttgtc tttaaggctt taatctctat
     1561 gtcatttatg cctaccatct ggcctgcttc catagctata tatcgctcaa gaagtgtttt
     1621 gctgtaaggg agcaatacag gcggaaaatc attgctgccg ttaataacgc tcttgcttgc
     1681 tatcagaact ccttttttgt caaagcctga gtaggtcatg gcatcgatta ttgcattagt
     1741 gtcatttgaa tggccaagat gagcgtgaga gactaggatt gctgttgtgg ctcttatgtt
     1801 aatattgttt tctgctgcgt tttttaatgc atttgggcct gggtcgagcc agaactggtt
     1861 ttcgtccacc tgaaggacaa tgcctcctga tgataggtgg ttttttccga tgacaagaga
     1921 tccctgtcct gtgcctaaaa aagtaagtga agctttcatc aaaatttaaa caatattatt
     1981 attaataaat ttttccttaa gagaaggttt aataaaatcc agattgaggc cagattttta
     2041 ctgagggttt ttctttattt tagaaaagct taaaaaacgc cttatattta cagtattggc
     2101 aggctatgcc gggaggttag cgctctcctg ttaccgtaaa tacgcttcag acggggctag
     2161 agcctgggaa gaggcatagg aaggcttatc ggtcttgaca tggacaatga gattctgtcc
     2221 tgtaggattg atatcttaag gaaccgcgcc aggccaggaa tggagcaacg cttacttctg
     2281 atgttgatgc ttatagggta gcggaatcaa ggaaatggca aggcaaaccg tttaggtttt
     2341 tcctgctgtc catttccagg ttgcctgcca cttttacatg ataaaagcga ttttttttga
     2401 tcttgatgga gttcttacta cagatggagt ttgaagtaga gacaacatca agaaatgtcg
     2461 tcaaacgttt ggggttggat tataattttg ttcttagggc ttataaggaa agcggccgag
     2521 tgtgggatct gtatataggc aaaataagct tggatgaaag ctggagagcg ttttattctg
     2581 cgcttggagt ttctatgagc tatgcccggt ttaaagacat gagaaaagaa gctttttcaa
     2641 atgctcctat cgatcaggaa atgacaaaga ttgcacggaa aataaaggca caaggatatt
     2701 ttcttggaat aataaccaat aatactaaag agagaatgaa tgcgataagt aagaattatg
     2761 gatttgatgg tctgtttgat tacatgataa catctgaaga tgtaatggca ttaaaaacag
     2821 acaagaaacc tttttatgag gggctgagac gtgctgatgt tactgccaac gaagttttgt
     2881 ttatagacaa cagcatgagt aattgtgagg ctgcaaagag aataggtata caagcgcttt
     2941 tctttgatca taagcaaaga aactaccaca ggctatacgg tgaattaatg aaaataggag
     3001 tgaaaatcta ggctttctgc ctgcttctga cataggaata aacatccaat gctatgacaa
     3061 ctacgataaa ggctatcgaa aatccaagag ggcttgcaag accaaatcct gtatcttctg
     3121 ccactgcaaa acctgttatc gacggattta tcaaaaaatt aaaaagaagg actgcgccta
     3181 ctgcgtttgc gataaataaa ggaagtttca taactaaaca taagcaagcc agtctttata
     3241 tttttcgttt tttccctgca caatgtccat aaaggcatcc cttagcttta gggtaatagg
     3301 tcctggtgca tgctttccaa cattgttgtt gtccaatgat tttatcgctg ttatctctgc
     3361 tgctgtgccg gtgaaatagg cttcgtctgc actgtagata tcttcttttt ttagcgtctt
     3421 ttcttctaca gtcatgccta agtctcttgc aatctttata accgagtctc ttgtgatgcc
     3481 tttgaggatg cctcctaatg ctggagtcag caattttcca tcctttacca tgaatatatt
     3541 ttctccaggg ccttctgcaa catttccttc gtagtccagc aataaggctt cgtcaaatcc
     3601 tttgtctttt acttcaactg aagcgaatat gctatttaca tagtgaccgc atatctttgc
     3661 ctctgaatct gtgctttttg gatggatcct tacataatcc gaggtcttta ccctaactgc
     3721 ttcttcccca agatattttc cccatggcca tactgcgatt atgcagttaa caggagcacc
     3781 ttcgggattt aaacccatct taccatagcc aaagaaacat agtgggcgta tgtaaccagc
     3841 ttttagtttg tttttcctta cggtttcctt tattgcctct gtcatctgct cttttgtgaa
     3901 aggtatcttc
//
LOCUS       DASRUK010000027        11792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1197130, whole genome shotgun sequence.
ACCESSION   DASRUK010000027 DASRUK010000000
VERSION     DASRUK010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 11792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11792
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197130"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VFF28_01180"
     CDS             complement(<1..255)
                     /locus_tag="VFF28_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44273.1"
                     /translation="MRPTYNYVYQQVAREGPTYYWNLYQGERVTQSIRMPPNGQDHFR
                     EFLGALEQRCLDVLARYPVRNEVVFGKEDFTDHLPLRTIEM"
     gene            complement(300..1073)
                     /locus_tag="VFF28_01185"
     CDS             complement(300..1073)
                     /locus_tag="VFF28_01185"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HZX44274.1"
                     /translation="MTKSLRDTKAAPGGRKGKREVSYNMIEDSPLPVGKACSVLNVSR
                     DAFYSWLKNPYQKPHDLETTRMIHEIAADFPRYGYRRITAELHRRNLIVNNKKVLGIM
                     RQENLLCRVKKGVPTTTNSAHSYPLYPNLVRNIQATGLNQVWVSDITYIEIGDGFVYL
                     ASITDIFSRKCVGWDLGRNIDAQLALNALNMAIMNRKHLGLSGLIHHSDRGVQYASHL
                     YVDCLRQHGIAISMCESGNPRENAYAVGRKNLPPFIEKL"
     gene            1143..1628
                     /locus_tag="VFF28_01190"
     CDS             1143..1628
                     /locus_tag="VFF28_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44275.1"
                     /translation="MKLFDYLMLSFEGKARYQDLHRAFSSSPEQLIQDLHAYDVQDTI
                     DRQYEEDSTFGDNLPEIIQKASAHFRECFPCFEEYPMHVRMYALGQSISTLRVLRRLG
                     IQNAEEDFKKRYGLTIRSYDYLNLLEDMVPGKPFYTTYLSYLINDRIQRRWKHQAARP
                     S"
     gene            1579..1986
                     /locus_tag="VFF28_01195"
     CDS             1579..1986
                     /locus_tag="VFF28_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44276.1"
                     /translation="MIGFNADGSIRLPDHLKKRKEDTENLLKNGRCMHIRKEIVSYQA
                     PKKCLLKLKLSDKITDNRFIDYIDKNTLTETPVKFLKVSDKEFNIEVGTNFRRCSDCT
                     KIISLLREFIHVIEDSGSCTYQRKDFCYEDHFD"
     gene            1988..3871
                     /locus_tag="VFF28_01200"
     CDS             1988..3871
                     /locus_tag="VFF28_01200"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZX44277.1"
                     /translation="MEFKGLVLDKFQEDAIRAIEKNHSVVVSAPTGSGKTLIADYIIC
                     RDVQKGIKVIYTAPIKALSNQKYKEFCESYGEDKVGLLTGDTSINPGARVVIMTTEVY
                     RNMAITKEIMEDVSYVVLDEIHYINDIERGTVWEESIIFSSSHIRFLCLSATIPNAKE
                     FAGWISKIKGHNVETVIHDERPVPLDIHFYDSELGITTLEDIREVKDIPDYRYVMGRQ
                     RQRTRTRPPNHIELLKDIKDKLPCFFFNFSRQGCQDLALELARAKVFDINPTISQITR
                     KKLESAPDEIRQLKSTQALRQCLPYGIAFHHAGLLPIIKELVEELFSKGLINVLYTTE
                     TFAVGINMPAKTVCFKSLRKFDGANFRTLNAKEFFQIAGRAGRRGIDTKGYCYAMMNR
                     RDFEYAQLKKITGKDTEPIISQFKLSVNTVLNLIKNHNDAQIEKILEMSFYTYQNGKI
                     GEIRNSFENIKRKLEKAGFVKDKNLTKKGEFSARIYGDEILTGDLFATDFYYDLNEYQ
                     IMLVVAALSYEQREKAEFYKTCPSHFLNQLKRKMDDKDERFHNLLPLTALVHPCYQGK
                     SMFEIMKNANFLEGDLLRFFRQMLDRLSQIINATEDERLKAMCKSCQQLILHSLADID
                     QTG"
     gene            4083..4253
                     /locus_tag="VFF28_01205"
     CDS             4083..4253
                     /locus_tag="VFF28_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44278.1"
                     /translation="METKLLDRNEIKPEKSELLESPIHIQFYDHIEKSWNEAMDFHVK
                     KMDISFDRLIRK"
     gene            4368..4757
                     /locus_tag="VFF28_01210"
     CDS             4368..4757
                     /locus_tag="VFF28_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44279.1"
                     /translation="MDKPAGISGRAEKTFNYLAAPHDLLLEQITALNFRQFRQGSLLK
                     INQSNHGPSFYVCNDCSYVASKEIDPEHIHMGGICYLITIDSTLIGKNPWDDRGIRIA
                     SKDLDSRLGPDEIARGYFFKVSAPYFG"
     gene            4760..4927
                     /locus_tag="VFF28_01215"
     CDS             4760..4927
                     /locus_tag="VFF28_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HZX44280.1"
                     /translation="MLNYQVYLARSLQLNSNLSPREITFRADHPFIFIIQQKEAGNIL
                     FMGKGMDPGKG"
     gene            complement(4933..5313)
                     /locus_tag="VFF28_01220"
     CDS             complement(4933..5313)
                     /locus_tag="VFF28_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44281.1"
                     /translation="MVAEQIDQSAEEISAERIIAPRQGGYMPSDLGELTVSPGNHFYM
                     VKGRSWPNFVQVYIADGAAYVGRPPSSLRRLGLEQLAKDLPQRPILVEYEGKPLLAIT
                     KSPSGSIMMIPYKKNTLALPSLRI"
     gene            complement(5438..5794)
                     /locus_tag="VFF28_01225"
     CDS             complement(5438..5794)
                     /locus_tag="VFF28_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44282.1"
                     /translation="MDKRIGNYSFIIGVIVAVVLGAFSWDALQPAKPYLASLLVLMGL
                     AVGFLNVTGKETKEYLLVAVVLVIAASMGGASATLGGVQYIGKYLSGIFTQVLAFVVP
                     ATVVVALKDIWALGQK"
     gene            5934..6596
                     /locus_tag="VFF28_01230"
     CDS             5934..6596
                     /locus_tag="VFF28_01230"
                     /inference="COORDINATES: protein motif:HMM:NF037269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogen output domain 1-containing protein"
                     /protein_id="HZX44283.1"
                     /translation="MLNPEIEAKIVEFIKNSPFGVTSSDIADYLSINRMTVAKYLAII
                     KVKAKVDFKQLGMAKLWFVPVILNKENYLDANLVNLAKELKDDKSKEKMKKAAIETAQ
                     IIEEQYRGFYNTSKLTYRQVLESLIDSQKKIGGNFTVVESNEDVIVLKNSKCPFGEPV
                     KHAPCLCATTSALCGVMTARNLGYSKVVLKKTIAKGSDICIIYIYLKKTKESEAAVGE
                     SI"
     gene            complement(6589..7065)
                     /gene="coaD"
                     /locus_tag="VFF28_01235"
     CDS             complement(6589..7065)
                     /gene="coaD"
                     /locus_tag="VFF28_01235"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HZX44284.1"
                     /translation="MTKLAIYPGSFDPITNGHLDIIKRALKIFDKVIIAVGESSDKKY
                     LFSAEERKDMIRKVTKGLNVEVDHFSGLLIDYAAKRKANAIVRGLRAVSDFDYEFQAA
                     LMNRKLNENIETVFIMTRGMYSYLSSSIVKEAAMHGAKLNGMVAPYVEKKLKGKFR"
     gene            complement(7062..7256)
                     /locus_tag="VFF28_01240"
     CDS             complement(7062..7256)
                     /locus_tag="VFF28_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014557020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44285.1"
                     /translation="MKLKSSKDNNKSRMEFVKYWAEYVRTHSDREWSKQQNVLINSQI
                     QSARAVKMTREQYLRIKGEL"
     gene            complement(7253..7831)
                     /locus_tag="VFF28_01245"
     CDS             complement(7253..7831)
                     /locus_tag="VFF28_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44286.1"
                     /translation="MRFIDNTLILDKEINELDRFVLKFIKVLEKHTDYVIISGYVSIL
                     LGRSRATEDIDIFIKPISRDSFKKLYDDLIRAGFWCLNAEGADKIYANLTDKIAVRFS
                     EKEKVIPNFEVKIVGNVLEADAFNSRIIAKLNIGDLKISSLERQIAFKRYYLGDEKDI
                     EDAKHIETLFKDRLDISLIENYKKMIESFKKK"
     gene            complement(7828..8397)
                     /gene="coaE"
                     /locus_tag="VFF28_01250"
     CDS             complement(7828..8397)
                     /gene="coaE"
                     /locus_tag="VFF28_01250"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HZX44287.1"
                     /translation="MIIGVTGVFGSGKTTVSNMFLNHCYLIINADEVGHRLLNREDIK
                     KSVVKQFGEGILTDGLVDRSKLKEIVFHNSEKLKELNKMHPEIIKEIKRMIKESGEDK
                     VVVDGALLIEAKGLSLVDKLIVVKIDRHEQIRRALKKGKYNREEIENIIKSQLSQEEK
                     LKYADYVVDNSGDLVNTEKQVEKIIEELK"
     gene            complement(8429..11017)
                     /locus_tag="VFF28_01255"
     CDS             complement(8429..11017)
                     /locus_tag="VFF28_01255"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HZX44288.1"
                     /translation="MIETMAKPYSEGEVRKILNPIVEKWFFSRFKTFSLPQLYGVMEI
                     HSRRNILVSAPTGATKTLTGFLSILNELVDSSVKGILENRVYCVYISPLKALNNDIQR
                     NLQEPLKQIEEIAGKELGIRVAVRTGDTETKERVDMLKHPPHILITTPESLAILLSSI
                     KFVEHLKHVDWFICDEVHALAENKRGVHLSLSMERLGALSPHMARVGLSATISPLSEV
                     AEFLVGYEDGKPRDCKIVDVQFLKETDLKVVSPVKDLIGVEHGLMHHSMYVLIDKLIQ
                     EHKTTLIFTNTRSATERVVHHLKEKFPANYSENIGAHHGSLSKVHRLDIETRLREGKL
                     KAVVCSTSLELGIYIGFIDLVICLGSPKSVARALQRIGRSGHRLSEVTKGRIIVLDRD
                     DLVECAVLLKDAVERKIDRIHIPSNCLDVLAQQIDGIAIQEKIHVDDLFKLCKQSYCY
                     RNLSRDDFMQVIEYLSGKYISLEDRFIYAKIWYDEETGMIGRKGKMGRVIYMTNIGTI
                     PEEVKVAVKIGEQTVGYIEEAFLEKMRTGDLFTLGGNVYEFKFSRGMVAQVKASVSRP
                     PTIPSWFSEMLPLSYDLAIDIGKCRKLICEKFCVGKSKEEILDFINSYLYVDKNAAES
                     IYNYFYEQYNYAQIPSDTRIIVEHWTDEKGKRYTIFHSLYGRRVNDCLSRAVAFAVGR
                     TQHKDVELGISDNGFYAGTTSRIDVMKAFKLIKSSELYKVMEMAIDQTEVLRRRFRHC
                     AARSLMILRNYKGRQKRVGRQQVSSTILLSAVRRISQHFSILKEARREVLEDLMDIEN
                     TRKVIEGIEQKKIKVEEINTTLPSPFALNLVTQGRTDIMKIEDRAEFLKRMHEMILAK
                     ISLKKR"
     gene            11131..11658
                     /locus_tag="VFF28_01260"
     CDS             11131..11658
                     /locus_tag="VFF28_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44289.1"
                     /translation="MNITLVNPLSKEERLQVTRLLAHDFSAMYNVCNGFGNLPPYSDY
                     AKHALHIAHTIYLLCNLDDIVLLGNSSDLIQRQDFGKFLRTIQSLSNGRIDIDFEHRD
                     FLTAPSILHTVITNLAKNSLKAQPEGNVIVRADYVSGFPENVTYTPDGARDYSEFIAL
                     RVLDKGKGFQKDKPL"
BASE COUNT         3470 a         2590 c         2269 g         3463 t
ORIGIN      
        1 catttctata gtcctaagag gcaaatgatc tgtaaaatct tcctttccga aaacgacctc
       61 gtttctaact ggataccttg ccaggacatc caggcatctc tgttcaagtg cgccaagaaa
      121 ctccctgaaa tgatcctggc cgttgggagg catccttatc gactgggtta ccctttcacc
      181 ttggtaaagg ttccagtaat atgtcggccc ctctcttgcc acctgctgat agacatagtt
      241 atatgttggc ctcatctagc aaagaatctt gaggttttat ttttaaatac tgtctttaac
      301 tacaatttct ctataaaagg tggtaaattc tttctgccaa ctgcataggc attctccctt
      361 ggattgcctg actcacacat gcttattgca atcccatgct gtcttaggca gtcaacatag
      421 agatgggatg cgtattgaac acccctatcc gaatggtgga tcaggccaga caagcccaaa
      481 tgcttcctgt tcatgatagc catattcaag gcgttcaatg ccaattgggc atcaatgttt
      541 ctgcctaaat cccagccaac gcattttctg ctgaatatgt ctgttattga cgcaagatat
      601 acaaatccat cccctatctc gatgtatgtg atgtcagaaa cccacacctg gtttaagcct
      661 gtagcttgga tatttctcac cagatttgga taaagcggat aagagtgtgc cgagtttgtg
      721 gttgtgggaa ctcctttctt taccctgcaa agcaggtttt cctgcctcat tatgcccaga
      781 accttcttgt tgttgacaat cagattacgc ctatgcaatt cggcagtgat ccttcgatag
      841 ccatatctgg ggaaatcagc agctatctca tggatcatcc ttgtcgtttc aaggtcatgt
      901 ggtttctggt atggattctt cagccagctg tagaaagcat ccctgcttac attgaggact
      961 gagcaggcct tgccgactgg aagtgggcta tcctctatca tgttgtagct cacctccctc
     1021 ttgccctttc ttccgcctgg tgctgcttta gtgtctcgta agctttttgt caaaaaccac
     1081 cagtcaccgc tcctttcagt cgctatagac tggtggcatg acggttaagt ggtttttgac
     1141 gcttgaaact ttttgactat ctgatgctat cttttgaggg aaaggcaaga taccaggatc
     1201 tccacagagc cttttcatca tctcctgagc aactaataca agaccttcac gcttatgatg
     1261 tgcaggatac aatagatagg caatatgaag aagatagcac ttttggtgat aatttgccgg
     1321 agataataca aaaagcttct gctcacttca gggaatgttt tccttgtttt gaggaatatc
     1381 ctatgcatgt caggatgtat gcactaggtc aatcaatatc taccctcagg gttcttagaa
     1441 ggcttggtat tcaaaatgca gaagaagact ttaagaaaag gtatggtctt actatcagga
     1501 gctatgatta cttgaatctt ttggaagata tggtcccagg taaacccttt tatacaacct
     1561 atctatcata tttgattaat gataggattc aacgcagatg gaagcatcag gctgccagac
     1621 catcttaaaa aaagaaaaga agataccgaa aacctgctta aaaatggcag gtgcatgcat
     1681 attagaaaag agatagtgag ctatcaggcc ccaaagaaat gccttctcaa actaaagctt
     1741 tcagacaaga taaccgacaa tcgctttatc gattatatcg acaaaaacac gcttacagaa
     1801 acgcctgtaa aattcctaaa ggttagcgac aaagagttca acatagaagt cggcaccaac
     1861 ttcagaagat gctctgattg cacaaagatt atcagcctat taagggagtt tatccatgtc
     1921 atagaagaca gtggcagctg cacctaccaa agaaaagatt tctgctatga ggaccatttc
     1981 gactaagatg gaatttaaag gcctagtcct ggacaaattt caggaagatg cgataagagc
     2041 gatagaaaaa aaccattctg tggtagtctc cgcaccaact ggctcaggaa agacactgat
     2101 agcagattac ataatctgca gggatgtcca aaaaggcatc aaggtcatat acacagcacc
     2161 gataaaagcc ctttccaacc aaaaatacaa ggaattctgt gaatcctatg gtgaagataa
     2221 agtaggcttg ctgacaggag acacttcgat taaccccggg gcaagggtcg tcatcatgac
     2281 cacagaagtc taccgaaata tggctataac caaagagatc atggaggatg tctcttatgt
     2341 cgtccttgac gaaatacatt atatcaacga catagaaaga ggcactgtct gggaagaatc
     2401 gataatcttc tcaagcagtc atatccggtt cctttgcttg tcagcaacaa ttcccaacgc
     2461 aaaagagttt gctggctgga tttcaaagat aaaaggccac aacgttgaga cagtcatcca
     2521 cgatgaaagg cctgttccgc tggatataca cttctatgac tcagagctgg gcataaccac
     2581 cttggaagat atccgagagg taaaagacat ccctgattac cgttatgtga tgggaaggca
     2641 aaggcagagg acaaggacaa ggccgccaaa tcatattgag ctcctaaagg acatcaaaga
     2701 taaactgcct tgctttttct ttaacttttc aaggcagggc tgccaggatt tggccctgga
     2761 gcttgcccgg gcaaaggtct ttgatatcaa tccaactatc agccaaatca caagaaaaaa
     2821 acttgagtca gcgccagatg agataaggca gctaaaatct acgcaggcat taaggcaatg
     2881 tcttccatat ggaatagcat ttcaccatgc aggcctcctt ccgataataa aggaacttgt
     2941 cgaagaattg ttctcaaaag ggttgatcaa cgtactatac actacagaaa cctttgccgt
     3001 tggcataaac atgcctgcaa agacagtctg cttcaaatca ttaaggaagt ttgacggcgc
     3061 aaatttcagg accttgaatg caaaggaatt cttccagata gcaggcaggg caggcagaag
     3121 gggaatcgac acaaagggct actgttatgc catgatgaac agaagggatt ttgaatacgc
     3181 tcagctaaaa aagattaccg gaaaagacac agaacctata atctctcagt tcaagctgtc
     3241 cgtaaacaca gtccttaacc tcataaaaaa ccataatgat gcacagatag aaaaaatact
     3301 tgagatgagc ttttacacct accagaatgg caagataggt gagataagaa acagctttga
     3361 aaatataaaa cgaaagcttg agaaagcagg cttcgtcaag gacaaaaacc ttacaaaaaa
     3421 aggcgagttt agcgcaagga tatacggtga tgagatactg acaggagatt tgtttgcaac
     3481 agatttctat tatgacctta atgaatacca gataatgctt gtcgttgccg ccttgtctta
     3541 tgagcaaagg gaaaaagcag agttctacaa gacttgtcca tctcacttcc ttaaccagct
     3601 aaaaaggaag atggacgata aggatgaaag gtttcataat ctgctgcctt tgacagcgct
     3661 tgtccatccc tgctatcagg gaaaaagtat gtttgagatc atgaagaatg cgaacttcct
     3721 tgagggtgac ctgttaaggt tctttcgcca gatgcttgat aggctttcac agataattaa
     3781 cgcgacagaa gacgaaaggc taaaggccat gtgcaaaagc tgtcagcagt tgatacttca
     3841 ttctttggct gatatcgacc aaacaggata atgcaaacag atactagcgt attctttcgt
     3901 tacccttgtc aaaagccaac aatgatgcag atgaggggga gggttggctt cgtcggagta
     3961 ccctcatagg gcaaagcgaa gctttgaccg tctgcatcat tattgttggc tcataaagca
     4021 tataaccttt tgtcaagctt gttttgagta aacttaataa agaagcacat tatacttacc
     4081 ctatggaaac caaattgctt gatagaaatg agataaagcc agaaaaaagt gagctattag
     4141 agtcaccgat acatattcaa ttctatgatc atatcgaaaa gtcatggaac gaagccatgg
     4201 atttccatgt aaagaagatg gatatttctt ttgatagatt gataagaaaa taattatttt
     4261 ttccgtctat tcggttattt tttagcctaa atttctgtca gacggttcgc cgaattgata
     4321 atttaaacga cagaaagctt atattatcga cttcgcaagc tgtcaatatg gataaacctg
     4381 ctggtatatc aggtagggca gagaagacct ttaattatct tgcagccccg cacgatcttc
     4441 tgttagagca gataactgca ctaaactttc gccaatttag gcaagggtca ttactaaaga
     4501 taaaccaatc aaatcacggt ccatcattct atgtctgcaa cgattgctct tatgtggctt
     4561 caaaagagat agatccagag catattcata tggggggtat ttgttatctc attactattg
     4621 actctacctt gatcggaaaa aacccctggg atgacagagg cattaggatt gcatcaaagg
     4681 atttggattc tagattagga cccgatgaaa tagccagagg ttactttttt aaggtcagcg
     4741 ctccatattt tggataagct tgctgaatta tcaggtatac cttgcaagaa gccttcaact
     4801 taactcaaac ctatcaccaa gagagataac tttcagggcg gatcatccgt ttatctttat
     4861 aatccagcag aaagaggccg gaaacatcct ttttatggga aaaggtatgg atccaggcaa
     4921 gggttaattt tattaaatgc gcaagcttgg aagtgccaaa gtatttttct tatagggaat
     4981 catcattata gaacccgatg gggatttagt tatagccagt aatggtttcc cctcatattc
     5041 tactaggatg ggtctttgcg gtaagtcttt tgctaattgt tccaggccaa gcctgcgaag
     5101 tgaagaggga ggccttccca cataggctgc cccatctgct atataaacct ggacaaagtt
     5161 aggccaagaa cgacctttca ccatataaaa atgattgcct ggagatacag tgagttctcc
     5221 caaatcgctt ggcatataac cgccttgtct gggtgctatt attctctctg cgcttatctc
     5281 ctcagcagat tgatctattt gttctgcaac caagtcctta acccgcctca tatcgtctga
     5341 tgttgcaaga actgttcggg tttttccggt tgggtattag aagaattgtt ttttatttat
     5401 aaacataacg tgagaaaata ataaaaaaat aaataattta tttctgtcca agagcccaaa
     5461 tgtctttaag tgcaacaaca actgttgcag gtacgacaaa tgccaagacc tgggtaaata
     5521 ttccgcttag gtattttccg atatactgta cgccgcccaa ggttgcactc gcaccaccca
     5581 tgcttgcagc aatgactagc actactgcaa caagcaggta ctctttggtc tcctttcctg
     5641 tgacatttag gaatcctact gcaagtccca ttaaaaccag caacgatgcc aggtatggct
     5701 tagcaggctg taaagcatcc caactgaatg cgcccaggac cactgcaacg attactccta
     5761 taatgaagga ataattgcct attctcttat ccatgtcatt ctcacctctt ttataccgct
     5821 aaggtattga ttatcaatac cacctagcaa atatataaag ctttcgctat catcaataat
     5881 caataaacat aagctttaaa taagtccagc ctctactgat gcaagggagt ggtatgctga
     5941 atccagaaat agaagcaaaa atcgttgaat ttatcaaaaa cagcccattt ggggtgacat
     6001 cttctgatat tgcagactat ttaagcatca ataggatgac agtggcaaaa tacctggcta
     6061 tcatcaaggt taaagctaaa gttgacttca agcagctggg tatggcaaag ctttggtttg
     6121 ttcctgtaat actcaacaag gagaactacc tggatgctaa tctggtaaat ctcgcaaagg
     6181 agctaaaaga tgataaatcc aaggaaaaga tgaaaaaagc agctattgag acagcccaga
     6241 tcatagaaga gcagtatagg ggattctata acacaagcaa gcttacctac cgccaggttc
     6301 ttgaatccct tatagacagc cagaagaaaa ttggcggaaa cttcacagtc gttgaaagca
     6361 acgaagatgt tattgtacta aaaaactcaa agtgcccctt tggcgaaccg gtaaagcatg
     6421 ctccttgcct gtgtgcaaca acctctgcgt tatgtggtgt gatgaccgca agaaacctgg
     6481 gatacagcaa ggtcgtgctt aagaaaacaa tagcaaaagg ctccgatatc tgtataatct
     6541 acatatatct taaaaaaaca aaggaatcag aagcagctgt tggcgaatct atctaaactt
     6601 ccctttaagc tttttctcaa cataaggggc aaccattccg ttaagctttg ccccatgcat
     6661 agcagcttct ttgacaatgg atgagctaag ataactatac attcccctag tcataatgaa
     6721 cactgtctca atattctcat tcagcttcct attcatcaaa gcagcctgaa actcatagtc
     6781 aaaatcagaa acagccctaa gccctctgac tatggcgttt gccttcctct ttgctgcata
     6841 atcgataagc agtcctgaaa aatggtcaac ctcgacattc aggccttttg tcaccttcct
     6901 tatcatgtct tttctttcct cagcagaaaa caggtatttc ttatcagaac tctcaccgac
     6961 agcgattatc accttgtcga atatctttaa agccctcttt atgatatcaa gatggccatt
     7021 ggttatcgga tcaaagcttc ctgggtagat tgctagtttt gtcatagttc accctttatc
     7081 cttagatatt gctcgcgcgt catttttaca gcccttgcac tttggatttg tgagttgata
     7141 agtacattct gctgttttga ccattctctg tcagagtggg tcctgacata ttctgcccaa
     7201 tacttgacaa attccatacg cgatttgtta ttatcttttg agctttttaa cttcattttt
     7261 tcttaaaaga ttcaatcatt tttttatagt tctctatcag agagatgtct agcctatcct
     7321 taaataacgt ttcaatgtgc tttgcatctt caatatcctt ttcatctcca agataatatc
     7381 ttttgaatgc gatttgtctc tccagcgatg aaatctttag atcacctata tttaactttg
     7441 caataattct actgttgaag gcatcagctt ccaagacatt gcctactatc tttacctcaa
     7501 aattaggtat aactttttcc ttttctgaga atcttactgc aatcttatcg gttaagttgg
     7561 catatatctt atctgcgcct tctgcattaa ggcaccaaaa tccggcccgg ataaggtcgt
     7621 catataactt cttaaaacta tctctagaaa ttggcttgat gaaaatatca atgtcttcag
     7681 tagctctgga tcttccaagc aatatggaaa catacccgct gattataaca taatctgtat
     7741 gtttttcaag aaccttaatg aatttaagca caaatctatc caactcattt atctccttat
     7801 caagaataag ggtattatct ataaacctca ttttagctct tcaatgattt tttctacctg
     7861 tttttcggta tttaccaaat cccctgagtt atccacaaca tagtcagcat atttaagctt
     7921 ttcttcctgc gaaagctgtg atttgataat attttctatc tcctctcgat tatattttcc
     7981 cttctttaag gcacgtctta tctgttcgtg cctgtctatc ttgaccacta tcagcttatc
     8041 gacaagagaa agtcccttgg cctcgataag taaagcgccg tcaacaacaa ctttatcctc
     8101 gccgctctcc tttatcatcc tttttatctc ttttattatc tcagggtgca tcttgtttag
     8161 ctcttttagc ttttcagagt tatgaaacac gatctctttg agcttgctcc tgtcaacaag
     8221 cccatctgta aggatacctt cgccaaactg cttgacaacg cttttcttga tatcttctct
     8281 gttaaggagc ctgtggccga cttcatcagc attgatgata agatagcagt gattcaggaa
     8341 catatttgaa acggttgttt tccctgagcc aaacactcct gtgacgccta ttatcataca
     8401 actctcaatt agaactaact ataaaaaatt acctcttttt taaagaaatc ttcgccagta
     8461 tcatctcatg catcctcttt aagaactcag ccctgtcttc tatcttcatg atatcagtcc
     8521 ttccttgggt cacaaggttc aaggcaaaag gcgaaggaag cgtggtgttt atctcttcaa
     8581 cctttatctt cttttgctcg attccttcga taaccttgcg agtgttctcg atgtccatca
     8641 ggtcttcaag cacctctcgc ctggcctctt tcaggataga aaagtgctga gaaatacggc
     8701 gcacagcgct tagtaatatt gtggaagaaa cctgctgcct tccgaccctc ttttgcctgc
     8761 ctttgtagtt tctaaggatc ataagggatc ttgcagcgca atgcctaaat cttcgtctga
     8821 ggacctctgt ctggtctatt gccatctcca tgaccttata gagttcgctg cttttgatga
     8881 gcttgaatgc cttcattaca tctatcctgc ttgtggttcc tgcataaaag ccattgtcgc
     8941 ttatgcctag ctccacatcc ttatgctgtg ttcttcccac tgcaaaagcg acagcccgag
     9001 ataagcagtc gttgacacga cgcccataaa gagaatgaaa tatcgtatac cttttgcctt
     9061 tttcatcagt ccaatgttcg acgattatcc gtgtatcaga aggtatctgc gcatagttgt
     9121 actgctcata aaaatagtta tagatagact cagcagcgtt cttatccaca taaagatagc
     9181 tgttgataaa atccagtatc tcctccttgc tcttccctac acagaacttc tcacatatga
     9241 gctttctgca tttccctata tcgatagcca agtcatatga tagtggaagc atctcagaaa
     9301 accatgaggg aatagtcggc ggcctgctga ctgaagcttt gacctgagct accatccctc
     9361 ttgaaaattt gaactcatac acattgcctc caagagtgaa caagtctcct gttctcatct
     9421 tttcaaggaa cgcttcttcg atgtagccaa cagtctgctc tccaatcttt acagcgacct
     9481 tgacctcttc aggtattgtg ccgatattag tcatatagat gacccttccc atctttcctt
     9541 tcctgcctat catcccagtc tcctcatcat accatatttt tgcatagatg aagcgatcct
     9601 caagtgagat atatttgccg gatagatact caatgacttg catgaagtca tctcttgata
     9661 gattacgata gcaataagac tgcttgcata gcttaaagag atcatcaaca tggatctttt
     9721 cttgtatggc aatgccatct atctgctgtg caaggacatc aaggcagttt gacggtatat
     9781 gtatcctgtc tatctttctt tcaacagcat cctttagcag tacagcgcat tcaacaagat
     9841 catccctgtc aaggacgatt atccttcctt ttgtcacttc acttagccta tgtccgcttc
     9901 ttcctatcct ttggagcgca cgtgcaacgg actttggcga gccaaggcat attacaaggt
     9961 cgataaatcc gatatatatg cccagctcaa gcgaagtgct gcaaacaaca gccttaagtt
    10021 ttccctcacg cagccttgtc tcaatatcca gcctgtgcac cttgcttagg ctgccatgat
    10081 gagcgccgat attttctgaa taatttgccg ggaacttttc tttaaggtga tgcacaacac
    10141 gttcagttgc acttcgtgtg ttcgtaaaga taagcgtcgt cttgtgttcc tggatgagct
    10201 tatcgatgag cacatacatg gagtgatgca taaggccatg ctcaacccct atcagatcct
    10261 ttaccgggct gacaacctta aggtcagtct cttttaagaa ctgtacgtcc acaatcttgc
    10321 agtcccgtgg ctttccatct tcatagccca caagaaactc agccacctca gaaagaggag
    10381 atattgtagc agaaagccca accctcgcca tatggggtga aagagccccc agcctttcca
    10441 ttgatagcga taagtgcact cctctcttgt tttcagcaag agcatgcact tcgtcgcaga
    10501 taaaccagtc gacgtgctta agatgttcca caaacttgat ggaagacaaa aggatagcga
    10561 gcgactcagg cgtggttata agtatatgcg gaggatgctt aagcatatcg accctttctt
    10621 ttgtctctgt atctcctgtc ctgacagcaa ctcttatgcc taactctttt ccagctatct
    10681 cctctatctg cttgagaggc tcctgtagat tcctttggat atcattgttt agcgccttta
    10741 gcggagagat atagacacag tataccctgt tttcaagtat cccctttaca gaagagtcga
    10801 ccagctcatt gagtattgaa agaaatcctg tcaaggtctt tgtcgcgcca gttggagcgc
    10861 taactagtat attcctgcga gaatgtatct ccatcactcc ataaagttgc ggcagtgaaa
    10921 aagtcttgaa cctggaaaaa aaccatttct cgactattgg gttcagtatt ttcctgactt
    10981 caccctcaga atagggtttc gccatagtct ctatcattac tcacaagaag aaatatttag
    11041 tttttaaagt tatgcttgca gatacttttt tatacagaat aataatcttt cacttgaggc
    11101 aacagcgctc agactgttcc tgagatgaat atgaatataa ccctagtaaa tccattgagt
    11161 aaggaagaaa gacttcaggt aacaagattg cttgctcacg atttttctgc gatgtacaat
    11221 gtgtgtaatg gctttgggaa tctacctccc tattcagatt atgcaaagca tgctctacat
    11281 attgcacata caatttattt actatgtaac cttgatgata tagtattact aggcaattct
    11341 tcagatctca ttcaaaggca ggactttggc aagtttctaa gaacaatcca gtcactttca
    11401 aacggaagga tagacatcga ttttgagcat agggattttt tgactgcacc ttcaattttg
    11461 catacagtta taacaaatct agcaaaaaac agcctaaaag ctcaaccaga aggaaatgtt
    11521 atagttagag cagattatgt ctctggattt cctgaaaatg ttacctacac tccagatgga
    11581 gctagagact attcagaatt cattgcctta agagtgttag ataaaggtaa ggggtttcaa
    11641 aaagacaaac ccttatagta aattaaattg aatatttctc attaaatgga ataagagata
    11701 gaactttttg tttgttgtac caaagacttc tgaagaattt cataattgcc ctttccattt
    11761 caggaatagt aggataaaac caattgccaa ca
//
LOCUS       DASRUK010000028         3547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1197309, whole genome shotgun sequence.
ACCESSION   DASRUK010000028 DASRUK010000000
VERSION     DASRUK010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197309"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..448
                     /locus_tag="VFF28_01265"
     CDS             194..448
                     /locus_tag="VFF28_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004557731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44290.1"
                     /translation="MDKLEQRVISIEERNRKVELDKAWETSLSRKLIITALTYITIVL
                     FFYAAALPNPFINSIVPTLGFAISTLSLPYFKKLWLKYRH"
     gene            complement(445..780)
                     /gene="panD"
                     /locus_tag="VFF28_01270"
     CDS             complement(445..780)
                     /gene="panD"
                     /locus_tag="VFF28_01270"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HZX44291.1"
                     /translation="MRWLLRSKIHRATVTQACTDYTGSITIDKELVERSGLIPGEKIL
                     VSSITSGNRLETYVIEGKTGEICINGAAAKLIKKGEEIIIMGFELSDKKIKPQIILVD
                     KGNKFVKYL"
     gene            complement(814..1452)
                     /locus_tag="VFF28_01275"
     CDS             complement(814..1452)
                     /locus_tag="VFF28_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44292.1"
                     /translation="MAQDILDELSSDDTREFMRDFNELIRVYNGIKDEYREGSKSIDK
                     DIKEYDSVVKLFNSKYKGIEIKFTHAKMGFSIFLKEKSAKDAFTNAASRLNGLKDIGA
                     NEFGKASVDEETKFTRELGKVKDKIFLSFVGQEGDSFVLEDCKGRVTLDFDEGICSEK
                     SPQCRLCCYYAVKDGIGDEDVYSRLCAMGFGEQPLLQKVRKFLQKFDPKLNE"
     gene            complement(1485..1973)
                     /locus_tag="VFF28_01280"
     CDS             complement(1485..1973)
                     /locus_tag="VFF28_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44293.1"
                     /translation="MDKKFIVLTIILLIIYGCSTDKTIPVVSKPSVQQDSPGTKLPII
                     EESDAVKPVASNPTAQKPAENKSAPKDTKTFTVTIQELKLSPQDLTINKGDTVIWKHE
                     DRWETDTKHYLTAHSNEFRSPVMKYGETFSHTFNTPGIYTYIDPIHKDRDLLYGKIIV
                     EQ"
     gene            2073..3269
                     /locus_tag="VFF28_01285"
     CDS             2073..3269
                     /locus_tag="VFF28_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HZX44294.1"
                     /translation="MKVIADLHIHSRHSRATSKDLTIENLEKYARIKGIGLLGTGDFT
                     HPKWIEEIKESLSEDGTGILRTKSGFPFILQTEISLVYSQAGKGRRVHLVLLAKDIET
                     SSQITSYLQSLGRVDYDGRPIFNKSCIEATEALKGIDDKIEIIPAHIWTPWFSLFGSM
                     SGFDSVKDCFGDQEKQIHALETGLSSDPAMNWRLSQLDRYSLVSSSDLHSFWPWRIGR
                     EATVFDLKDLTYDSLIKALRTKEGLDSTIEVDPNYGKYHFDGHRNCHICMAPKHSEKV
                     NNICPKCGKQLTIGVLHRVEELADRPEGFVPKDAKPYKSIIPLSDIISAMIKSPVASK
                     KTWKVYNDLMQKFGDESSVLLYAAEEDIAKVVEGKVAKAILDNRQGKITVRPGFDGEY
                     GKPLID"
     gene            complement(3396..>3547)
                     /locus_tag="VFF28_01290"
     CDS             complement(3396..>3547)
                     /locus_tag="VFF28_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HZX44295.1"
                     /translation="ALRELVVIRKIIGSLRNKKGARIVETIMTMITTWRQRGLDTFSE
                     LRDSI"
BASE COUNT          981 a          747 c          728 g         1091 t
ORIGIN      
        1 tgttggcaat tggttttatc ctactattcc tgaaatggaa agggcaatta tgaaattctt
       61 cagaagtctt tggtacaaca aacaaaaagt tctatctctt attccattta atgagaaata
      121 ttcaatttaa tttactataa aaattctata tttaaccaaa acaaataaat aatcaggctg
      181 ttgataagga tccatggaca agttagaaca aagagtaatc tcaatcgaag aaaggaacag
      241 aaaagtagag ctggacaaag cttgggagac aagcctttca agaaagttga taatcactgc
      301 cctcacttat atcaccatcg tcctgttctt ctatgcggca gccctcccaa atcctttcat
      361 caattcgatt gtccctacat taggctttgc gatttcgacc ctatctctcc cttatttcaa
      421 gaagttgtgg ttaaaataca ggcactaaag gtatttcaca aatttgttcc ctttatctac
      481 caggattatc tgcggcttga ttttcttgtc agaaagttca aagcccatta ttattatctc
      541 ttctcccttt tttataagct tggcagcagc tccgtttatg catatctcgc cagtttttcc
      601 ctcgatgaca taggtttcaa gcctgtttcc agatgtgatg cttgacacaa gtattttttc
      661 tccaggtata agtccagatc tctccacaag ctccttatct atcgttatgc ttccggtata
      721 atctgtgcat gcctgggtaa cagtcgctct gtggatcttt gacctaagta accatctcat
      781 gatcaagaat aaattgccat gcattaataa aacttactca ttgagctttg ggtcaaattt
      841 ctgcaggaat tttctgactt tctgtaaaag aggttgctcg ccaaatccca tggcgcatag
      901 tctgctgtag acgtcttcat ctcctatccc atcctttacc gcataatagc agcaaagcct
      961 gcattgagga gacttctcgc tgcatatgcc ttcatcaaaa tcaagtgtaa ccctgccttt
     1021 gcagtcttca aggacaaatg aatctccttc ctggccgaca aatgacaaga aaatcttatc
     1081 cttgaccttg ccaagctcac gtgtaaattt tgtttcttca tcgactgaag ccttgccaaa
     1141 ttcgtttgcc ccgatatcct taaggccgtt caaccttgag gcagcatttg tgaatgcatc
     1201 tttagcgctt ttttccttaa ggaatatgga aaagcccatc ttggcatgtg tgaactttat
     1261 ctctatgcct ttgtattttg agttgaaaag cttgacgacg ctgtcatatt ccttaatatc
     1321 tttgtcgata gacttgctgc cttcccggta ttcatcttta atcccgttat aaactcgtat
     1381 cagttcgtta aagtcacgca tgaactctct ggtatcgtca gaggaaagct catcaaggat
     1441 atcttgggcc atagtagagg tcacatgatg cagatattta aaccttattg ttcaacgatt
     1501 atcttgccgt aaagaaggtc cctgtcttta tgtatcggat cgatataggt gtatatgccg
     1561 ggagtgttga aagtatggct gaatgtctcg ccgtacttca tgacagggct tctaaactca
     1621 tttgaatggg cagtcaggta atgctttgtg tctgtttccc atctatcttc gtgtttccat
     1681 atcactgtgt cgcctttatt tatcgtcaaa tcttgaggcg atagtttaag ttcctggata
     1741 gttacggtaa atgttttagt atcttttggg gctgatttat tctcagcagg tttttgagcg
     1801 gttggatttg aagcgaccgg ctttacagca tctgattctt ctataatggg cagctttgta
     1861 ccaggtgagt cttgctgtac agaaggtttg cttacaacag gaatggtctt gtctgtcgag
     1921 cagccgtata tgataagaag gatgattgta aggacgataa actttttatc catatttggg
     1981 ttatacctgc aaaagagtat ataaatattt ctactgaaga gttataatag cgaatcacaa
     2041 attttttata ccgaccaaaa ttaccctccc tgatgaaagt tatcgcagat cttcatatcc
     2101 attctaggca ctcaagggct acatcaaaag acctcactat agaaaacctt gagaagtatg
     2161 caaggataaa gggtattggc cttcttggta caggcgattt tacccatcca aaatggatag
     2221 aggagataaa agagagcctc tcagaggatg ggacaggtat cctcaggaca aaaagcggtt
     2281 ttccattcat ccttcagaca gagatctctt tggtgtacag ccaggcaggc aaaggcagga
     2341 gggtccacct tgtccttctg gcaaaagaca tagagacttc gtcgcagata acaagctatc
     2401 ttcagagcct tggaagggtg gattatgatg gcaggcctat ctttaataaa tcctgcatag
     2461 aggccacaga ggctttgaaa ggtatagacg ataagataga gataattcct gcccatatat
     2521 ggactccatg gttttctttg ttcggctcta tgtctggttt tgactcggtc aaggattgtt
     2581 ttggtgatca ggaaaagcaa atacatgcac tcgaaactgg actcagcagc gaccctgcga
     2641 tgaactggag gctttcgcag ctcgatagat attctttggt gtcatcgtcg gaccttcatt
     2701 ctttctggcc ttggaggata ggaagggaag cgaccgtttt tgacctaaaa gatctaacct
     2761 atgattctct cataaaagcc ctgaggacaa aagaaggcct cgactcgacg atagaggtcg
     2821 accctaacta tggcaaatac cattttgatg gccataggaa ctgccatatc tgtatggccc
     2881 ccaagcattc agagaaagta aacaacatct gcccaaagtg tggaaagcag ttgaccattg
     2941 gggtcttgca tagggttgag gagctggcag acaggccgga aggttttgta ccaaaagatg
     3001 caaagcccta caagagtata atccctctct cagatataat ctcggcgatg ataaagtcgc
     3061 ctgtagcctc aaaaaagacc tggaaggtct ataatgacct gatgcaaaag ttcggcgatg
     3121 aatcaagcgt tcttctatat gcagcagaag aggacatcgc taaagtcgtt gaaggaaaag
     3181 ttgcaaaggc tattcttgat aaccgtcagg gaaagattac tgtccggcct ggctttgacg
     3241 gcgaatatgg aaagccgctg atagattaag tgctggattt atcatatgtt gagatgccgt
     3301 tttctcgtcg agaaattagc catttttgcg atattggcct ttttcaccgc cggatgatta
     3361 ctattttacc gtcgattttt tcgtataagg taagctcaga ttgaatctct tagctcagaa
     3421 aatgtatcta atcctctttg tctccacgtg gttatcattg tcattatagt ttctactatt
     3481 cttgctccct ttttattgcg cagtgaacct attatcttcc gaataaccac caactccctc
     3541 aatgccc
//
LOCUS       DASRUK010000029         2348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_364811, whole genome shotgun sequence.
ACCESSION   DASRUK010000029 DASRUK010000000
VERSION     DASRUK010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364811"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..562)
                     /locus_tag="VFF28_01295"
     CDS             complement(143..562)
                     /locus_tag="VFF28_01295"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HZX44296.1"
                     /translation="MSEVPVDRNTRWECQRCGECCKGMILSKERNLSVYSGKGAVCRH
                     LTDGGCSNYNQRPFICKLYPFVVNLDKAVCREVFDPSISFLPENLGIHSGCPGYGKGK
                     RVFGNRSLTRSFSRLGEELALKAKKSLETGEDISKFI"
     gene            complement(563..1297)
                     /locus_tag="VFF28_01300"
     CDS             complement(563..1297)
                     /locus_tag="VFF28_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HZX44297.1"
                     /translation="MKGKIALITGAARGLGKEMALVFAGEGCDIIINDIEPMKETALA
                     TASEIAAKGVKCEFIAADVSNFESCRVLADKIKEKFGKVDILVNNAGITKDRTLKKMT
                     PEEWYPVINVNLNSLFNVTKNVLDIIPDGGRIVNISSVAGISGNFGQTNYSSTKAGVI
                     GFTKSLAKELGKRKITVNAIAPGFIKSDMTDKIPMELLSNIIELIPLKEIGLPRDVAN
                     AVKFLASDDARYISGMVIKVDGGLSF"
     gene            complement(1299..>2348)
                     /locus_tag="VFF28_01305"
     CDS             complement(1299..>2348)
                     /locus_tag="VFF28_01305"
                     /inference="COORDINATES: protein motif:HMM:NF014820.4"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HZX44298.1"
                     /translation="ADMGMGKVDAVICSSLEWFYTGETQRHFASLLASTFKARIPIIR
                     VPGACAGGGTALWTTLRMMNNSELNNVLVIGAEKLLGHTRNSEKITEEFSMAAESCWE
                     QHEGMNFPAENALAAQAYLNKYPETTMDDLALISQKNHENAYLNPKAFFYGKKVGLDE
                     IKNSPIVTSPLRLFDCSISVDGSAAVLLTKDKTDVKIAGSGYCADYLAPFERESLTSW
                     EGVVTAANQAFSNAGIDRGSIDFAEVHDAFSIVELLAYEDLGFCKRGEAYKYIRDGYF
                     KMNGKLPVNVSGGLKAKGHPVSATGLGMIYEMTKQMRKEAGDRQLSHINYSLSHNIGG
                     AGGNTACHILKRVGG"
BASE COUNT          611 a          571 c          447 g          719 t
ORIGIN      
        1 aaagctcatg ggctgcataa gaaatgaaaa tggcagaagg tttattggaa atgtgatgtc
       61 ggcaatggaa acatggaatc agcaagggaa aaatgtctat gaaaatctga tttcatttgc
      121 tagctgactg aatacttacc atttagataa atttggatat atcttcgcct gtttccaggc
      181 ttttctttgc tttaagtgca agttcttctc ctaatcttga gaaactcctg gttaatgatc
      241 tattcccaaa aacacgtttc cctttcccat agcccgggca tccagaatgt atgcctaaat
      301 tttcaggcaa aaaagagatt gaagggtcaa aaacctctct acaaacagcc ttgtctagat
      361 tgacaacaaa aggatatagc ttgcagataa atggcctttg gttatagttt gagcagccgc
      421 catctgtaag atgcctgcaa accgctcctt ttcctgaata aacagaaaga ttgcgctcct
      481 ttgacagtat catgcctttg cagcactcgc cgcatctttg gcattcccaa cgggtatttc
      541 tgtcaacagg aacttcactc atctaaaaac taagtccccc atcgaccttg atgaccatcc
      601 ctgatatgta tcttgcatcg tcagaagcaa ggaactttac agcatttgca acatctcttg
      661 gaagtcctat ctctttaaga gggataagtt cgattatatt ggatagtaat tccatgggta
      721 tcttatcagt catgtctgat ttgataaatc caggtgcgat tgcattgaca gtgatttttc
      781 ttttccctag ctcttttgcc agggattttg taaagccgat gactcctgct ttcgtagatg
      841 aatagtttgt ctgcccgaag ttgccagata tgccagccac agatgatatg ttcactatcc
      901 ttccgccatc agggataata tcaaggacat tctttgttac gttaaaaagg ctgttaaggt
      961 taacatttat cacaggatac cattcttcag gtgtcatctt tttaagggtc ctgtccttgg
     1021 ttatgcctgc attgttgaca agtatgtcga cctttccaaa tttttccttt atcttatctg
     1081 ccaaaaccct gcagctctca aaattagaca catctgcagc gataaattca catttaacgc
     1141 cttttgctgc aatctccgac gcagtggcaa gcgcagtctc tttcataggc tcgatgtcat
     1201 tgatgatgat gtcacatcct tcacctgcaa aaacaagtgc catctctttt ccaaggcccc
     1261 ttgcggcgcc ggttatcaaa gcgatttttc ctttcatttt accctcctac tcttttgagt
     1321 atatggcagg cagtgtttcc gccagctcct ccaatattat gggaaagtga ataatttata
     1381 tgagaaagct gcctgtctcc tgcttctttt ctcatctgtt ttgtcatctc atatatcata
     1441 cccaaccctg tcgcggatac aggatgtcct tttgctttta acccgccaga aacattgacc
     1501 ggcagcttgc cattcatctt aaaataacca tcccggatat acttatacgc ctctcctctc
     1561 ttacaaaacc ccagatcttc atatgcaagt aattctacga ttgaaaatgc atcatggacc
     1621 tctgcaaagt cgatgcttcc cctatcgatg cctgcattag aaaaagcttg gttagctgca
     1681 gtcaccacac cctcccatga tgtaaggctc tccctttcaa aaggagcaag ataatcagcg
     1741 cagtatccag agccagctat ctttacatca gtcttatctt tggtaagcag tacagcagca
     1801 gacccatcta cacttatgct gcagtcaaat agccttagag ggcttgtcac gatgggtgag
     1861 ttctttatct catcaagccc caccttcttt ccataaaaga atgcttttgg atttagatat
     1921 gcgttctcgt gatttttttg gctgataagt gcaaggtcat ccatggttgt ttcaggatat
     1981 ttattgagat aagcttgtgc tgcaagagcg ttttctgccg ggaagttcat cccttcatgc
     2041 tgctcccagc agctctcagc agccatgctg aattcctctg tgattttttc gctgtttctt
     2101 gtatggccca ggagcttctc agcccctatt acaagcacgt tgttcagctc agaattgttc
     2161 atcatccgca aagttgtcca aagcgcagtc cctcctccgg cgcacgcacc aggcaccctg
     2221 atgataggaa tcctcgcctt gaaggtggat gcaagaagag aagcaaaatg cctctgggtc
     2281 tctcctgtat aaaaccactc tagagacgag catatcactg catctacttt gcccatgccc
     2341 atatcggc
//
LOCUS       DASRUK010000030        10042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1702670, whole genome shotgun sequence.
ACCESSION   DASRUK010000030 DASRUK010000000
VERSION     DASRUK010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 10042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10042
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1702670"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VFF28_01310"
     CDS             complement(<1..498)
                     /locus_tag="VFF28_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HZX44299.1"
                     /translation="MSEEIIFSGFGGQGVVLAANVIAQTAVYEGKNMAAMVSYGAEMR
                     GGTSSSTLIISEKEIYSPVVEHPSVAVVLNQPSLDLYEPLVKKGGLLIVNSSLTDRKA
                     ERKDIDVIYIPATDIANTLGNVKIANMILVGALVARKRLVSIENVLSILPKAFPKNKQ
                     EFVEIN"
     gene            complement(491..1216)
                     /locus_tag="VFF28_01315"
     CDS             complement(491..1216)
                     /locus_tag="VFF28_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZX44300.1"
                     /translation="MKLAMPQSLKNVGIYYCAGCGHGVAHRLVGEVIDELRIREKTIG
                     TAPVGCAVLLYNYFNFDIIECAHGRPPAVATAMKRLMPEKIIFTYQGDGDLAAIGTNE
                     TIHAANRGEGYTAIFVNNAVYGMTGGQMAPTTLLSQKTTTSPFGRRSSVEGFPLRMCE
                     LLCTLEGPKYIERVSLDSVKNIIKAKAAVKKAFSNQLEGKGFSFVEILAPCPTNWKMT
                     PVESLEYVKKMQNYFPLGVYKDE"
     gene            complement(1213..2265)
                     /gene="vorB"
                     /locus_tag="VFF28_01320"
     CDS             complement(1213..2265)
                     /gene="vorB"
                     /locus_tag="VFF28_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HZX44301.1"
                     /translation="MTKQLMKGNEALAEAALQAGCKFYAGYPITPQNEIPEYFAKRGP
                     ELGCQFIQSESEIAAINMIFGASAAGFRCMTSSSSPGISLKQEGISYIAGGELPCVIV
                     NMVRGGPGLGNIRASQADYFQAVKGGGHGDYHMIVIAPSNVQEMYDLTMNAFDFADHY
                     RNPVMILGDGIVGQMFEPVEIKSYKPVLKLPAKNFKLDGCAGREPRIVRTLLLAPADA
                     LAKHNEHLVQKYERIKKELCLHEEYMTSDAEIVIVAYGITARIAKGAIEKARKEGIKV
                     GMIRPITVWPFPSEAIAKSGAKKFLAVEMSNGQLVEDVKLSLFGKPGVSVEHFGTGGG
                     WYPSPSNIYDKIKEII"
     gene            complement(2351..2554)
                     /locus_tag="VFF28_01325"
     CDS             complement(2351..2554)
                     /locus_tag="VFF28_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZX44302.1"
                     /translation="MVKITINKERCKECSFCVAVCPIKLIKISSKLNKRGYHYAELID
                     DKKCTGCAQCYQMCPDMCITIEK"
     gene            2658..2927
                     /locus_tag="VFF28_01330"
     CDS             2658..2927
                     /locus_tag="VFF28_01330"
                     /inference="COORDINATES: protein motif:HMM:NF039498.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5676 family membrane protein"
                     /protein_id="HZX44303.1"
                     /translation="MAKKVDPNTVALSLAGVSAIFSLSCALIVSLAPDFSLRLARALF
                     HGLEISGIAAPTINDILAGLGGVTIIAILSGWLFALFYNFITTKR"
     gene            complement(3376..3741)
                     /locus_tag="VFF28_01335"
     CDS             complement(3376..3741)
                     /locus_tag="VFF28_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HZX44304.1"
                     /translation="MKALLAILLLGSIILSGCSQKPQVKEFVMESYTEIIDEKYYPKF
                     SPNEITVNKGDTVRIKITNTKGTHDFKIDEFNAYTETPLNEQVAVEFAADKAGSFEYY
                     CTKPGHRANGHWGTLKVTS"
     gene            complement(3754..4134)
                     /locus_tag="VFF28_01340"
     CDS             complement(3754..4134)
                     /locus_tag="VFF28_01340"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HZX44305.1"
                     /translation="MEKEKIVEMAIIILIVAAVAFGAIRIFGLLGADAPTASAVKDTT
                     EIKVSAFRFGYSPEVVTVSKGDKVKLVIDNTDTTHGIRIPELGLAGNEVIEFTADKTG
                     EFTWYCNVMCGSGHSQMKGKLIIR"
     gene            4261..4563
                     /locus_tag="VFF28_01345"
     CDS             4261..4563
                     /locus_tag="VFF28_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44306.1"
                     /translation="MSTINEVLSISASVALLLAVYFSYKLSRETKHERYWLLLSLGLF
                     IFAVHHWFMIIFQLGNIPFFILELLEYLTSIVAGILIAYSTYGLYKSMRAVNQRVR"
     gene            4560..4835
                     /locus_tag="VFF28_01350"
     CDS             4560..4835
                     /locus_tag="VFF28_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44307.1"
                     /translation="MIPKSILFYWSKGADVRRKILKILSDCEKQAKPCFLNIAAKKLS
                     LTHVAVKKHIDLMVEEGYIEPINPKGKPVFLKLTPLGKGVIKELEKR"
     gene            complement(4839..5141)
                     /gene="cutA"
                     /locus_tag="VFF28_01355"
     CDS             complement(4839..5141)
                     /gene="cutA"
                     /locus_tag="VFF28_01355"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HZX44308.1"
                     /translation="MIRVHITCKDEEEAKSVSEHLLKKRLIACSNMHPISSMYWWKGK
                     IEKRTEFVIDAKAPSANWDKIQNEVRNVHSYEVPCIIRIEASANKECEEWIEGESR"
     gene            5194..6135
                     /locus_tag="VFF28_01360"
     CDS             5194..6135
                     /locus_tag="VFF28_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44309.1"
                     /translation="MHNKYIHSGILGIFIYNEHFNVLEKHLFNNNEQYLNGKKLEEEM
                     HKKYSNLKKPEGKELKKILELFMGKEYFPDFRKNALSITRLEIKHSVKDDLLIMQAIN
                     CVDETSKTINILIKRLREWYAIYNPELEHEIENQEKFVELVLTKSKKELLKEIQVDEK
                     ASMGADLAKEDIEPIKALAKKAEELFDLKSVEEAYLEATMKKVCPNLLAVAGSTIGAR
                     LMEQAGGLKNLMLFPASTVQLLGAEKALFRHMKTKARAPKYGFIHEHPIISRAKKSMH
                     GKLARSLALKISIAVKIDYFHGKFIGDKLLKELEDIR"
     gene            6132..6797
                     /locus_tag="VFF28_01365"
     CDS             6132..6797
                     /locus_tag="VFF28_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004577844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrillarin-like rRNA/tRNA
                     2'-O-methyltransferase"
                     /protein_id="HZX44310.1"
                     /translation="MKATSIFEVYADEKGRPYTVNAAPGIQVYDEKLVTLKGVEYREW
                     NPRKSKLAASIMKGSPNIFMRKGDVILYLGCSTGTTVSHVSDIVGDEGFIFALDFAPR
                     VMREMVFTAEKRKNIAPLLADANKPEAYAGKVTMADVIYQDIAQRSQVEIFLKNVNLF
                     LKKGGYCLLAVKARSIDVTKKPRVIFSDIRQRLEKELTIIDYRTLEPFQMDHCMFICK
                     NNK"
     repeat_region   6871..7429
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=6871..6900
                     /rpt_unit_seq="atttcaatcctactctagttcaattctaac"
     gene            complement(7527..7790)
                     /gene="cas2"
                     /locus_tag="VFF28_01370"
     CDS             complement(7527..7790)
                     /gene="cas2"
                     /locus_tag="VFF28_01370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HZX44311.1"
                     /translation="MYYLLVYDVDVSKVNKIYKYLKRYMNWIQNSVFEGELGDADFLE
                     VKKKLNQWVDKETDSILIFKFRDKHSFEKEVIGRERNELSNIL"
     gene            complement(7792..8778)
                     /gene="cas1b"
                     /locus_tag="VFF28_01375"
     CDS             complement(7792..8778)
                     /gene="cas1b"
                     /locus_tag="VFF28_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011953726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1b"
                     /protein_id="HZX44312.1"
                     /translation="MQKQNYYITQSGTIKRKDSTVWFENEKIRKAIPVNSIDTIYCLG
                     EVSINSKLLVFLAKQGIILHFFNYYGYYSGSFYPKESLVSGSLLVNQVNYYTDSGKRL
                     FLAKSFISGTIQNLIRILEHYRKHSKEVGDIMDKLAKGVPKLDSCSTIPELMNVEGCA
                     WNDYYSSYEEILKEEFEVRTRRPPHNEVNCVISFLNSLLYTTTLSEIYHTQLNPTISY
                     LHEPFERRFSLALDISEMFKPLIVQRAMLKLFNKSELKDNHFRKDLNSCMLNEDGRRI
                     VLKEFDDRLKLTIEHPELKRLVSHRRLIRLESYKLVKHLIGDKTYSPFVIWW"
     gene            complement(8780..9286)
                     /locus_tag="VFF28_01380"
     CDS             complement(8780..9286)
                     /locus_tag="VFF28_01380"
                     /inference="COORDINATES: protein motif:HMM:NF014040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HZX44313.1"
                     /translation="MVKNLYCTQFPGYMALTATHINYFFTCKRKLWLFSKKITCEHES
                     ELVKIGKVHHQDIEKESLQIDNVKIDSLREGKVFELKKKNTAPEAAKYQVLYYLLKLK
                     EKGIFTTGTIKYKENNRLEEVELTQANEDLLNNAIRQAELICQEQVPEAKRNKYCRLC
                     SYYELCFS"
     gene            9333..9914
                     /locus_tag="VFF28_01385"
     CDS             9333..9914
                     /locus_tag="VFF28_01385"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HZX44314.1"
                     /translation="MKTDQKIIKALIKSPDTNTISALSGEIGISRQGVFKVVKRLELE
                     RLLRLSKVGKGRTSIFVIKLNWQNPILEKQAAIILLDEAQKNQRWITNFIELEKNVKF
                     TLIFGSVLTSPKEAKDIDLINVASDFITIDKIINATQKTQTKKIHALNLTEAEIREEL
                     AKGNPALTEAIKKGVVLFGHENFIKFIRGLNGS"
BASE COUNT         2932 a         2199 c         1918 g         2993 t
ORIGIN      
        1 gtttatctcg acaaactctt gtttattttt cggaaaagct ttcggcagta tagaaagaac
       61 attctcaatg cttacgagtc tcttcctcgc gactaaagcg ccgacaagta tcatatttgc
      121 tatcttcaca ttgcccaggg tattggcaat atcagtagca ggaatataga ttacatcgat
      181 atccttcctc tcagcctttc tgtcagtaag gctgctattt actattagaa gccccccttt
      241 ctttaccaga ggctcataaa gatcaagcga aggctggttt aacacaacag caacagacgg
      301 atgttccaca actggcgaat aaatttcttt ttcagaaatt atcaaggtag agcttgatgt
      361 cccgcccctc atctcagcgc catacgaaac catcgcagcc atgtttttac cttcatagac
      421 agcagtctgg gcgataacat tggcagcaag gacaaccccc tgccctccaa atcctgaaaa
      481 tattatctct tcactcatct ttatacactc cgagtggaaa ataattttgc atcttcttga
      541 catattccag cgattcaaca ggcgtcatct tccagttggt cgggcatggc gcaagtatct
      601 cgacaaacga gaatcctttc ccttccagtt ggttggaaaa agccttcttg acagcagcct
      661 tggccttgat gatatttttg acagaatcaa gcgacactct ttcaatatac tttggcccct
      721 caagtgtgca aagaagctcg cacatcctta gaggaaatcc ctccaccgaa ctcctcctgc
      781 caaaaggcga agtcgttgtt ttctgcgata gaagagttgt cggagccatc tggccgcctg
      841 tcatcccata cacagcattg ttaacaaata ttgcagtgta tccttcccct ctgttagcag
      901 catgtatagt ctcgttggtt cctatggcag caagatcccc atcgccctga tatgtaaaga
      961 ttatcttttc aggcatgagt cttttcatcg ctgtcgcaac agcaggaggt cttccatgcg
     1021 cgcattcgat gatatcaaaa ttaaagtaat tgtagagaag cacagcgcag ccaacaggcg
     1081 cagtccctat tgtcttctcg cgtatcctca gttcatctat cacttcacct acaaggcgat
     1141 gggctactcc atgtccgcag ccggcgcaat aatagattcc gacgttcttc agtgactggg
     1201 gcattgcaag cttcatatta tctcctttat cttatcatag atattgctgg gtgatggata
     1261 ccatcccccg ccggttccaa aatgctcgac agatactccc ggcttgccaa acaaggaaag
     1321 ttttacatcc tcaacaagct ggccgtttga catctctact gctaggaatt tttttgcacc
     1381 agatttggct atagcctcag aaggaaacgg ccacacagtg attggcctta tcatcccaac
     1441 cttgattcct tcttttcttg ccttttcgat ggctcctttg gcgatccttg cagttatccc
     1501 ataagcgaca atcacaattt cagcgtcaga agtcatatac tcttcatgaa ggcatagctc
     1561 tttttttatg cgctcatact tttgcacaag gtgctcattg tgcttggcaa gagcatcagc
     1621 aggagcaagt aaaagtgtcc ttactatcct tggctctctt ccagcgcatc catctagctt
     1681 gaaatttttt gcaggcaatt tgagcactgg cttatacgat tttatttcga caggctcaaa
     1741 catctgccca actatgccgt cgccaagtat catcaccgga ttcctgtaat gatcagcaaa
     1801 gtcaaaagcg ttcatggtca ggtcatacat ctcttgtaca ttggaaggag cgatgactat
     1861 catatgataa tctccatggc ctcctccctt cacagcctgg aaatagtcag cctgagacgc
     1921 ccttatattg ccaagcccag gccctcccct taccatattt actatgacgc aaggcagctc
     1981 tcctcctgcg atatagctta tcccttcttg ttttagggat attcccggag agcttgacga
     2041 agtcatgcat ctaaatccag cagcagaagc cccaaatatc atattgattg cagcaatctc
     2101 agactccgac tggataaact ggcagccaag ctcaggccct ctctttgcaa aatactcagg
     2161 tatctcgttc tgaggggtta taggatagcc tgcataaaac ttgcagcctg cctgaagagc
     2221 agcttcagca agcgcttcat ttcctttcat cagttgcttt gtcattattc acgccaacaa
     2281 ttattgttgg ctgtatcata atcctcaatt cagcattatt gttggcttgc ctattagttt
     2341 aacaacattc tcatttttcg atcgtaatgc acatatcagg gcacatctgg tagcattgtg
     2401 cgcagccggt gcacttctta tcgtcgatta attcagcgta atgatatccc ctcttgttta
     2461 atttagaaga tatctttata agttttatag ggcatacggc aacacaaaaa gagcactctt
     2521 tgcaccgttc cttgtttatg gtgattttta ccataataag gctagttaac cttaaatcta
     2581 ttaaaaaacc tttggtttaa gccagctatt taccaatcgc cgcaacattt aaaaaggcag
     2641 gccatcatga tatgcctatg gcaaaaaaag ttgacccaaa cacagttgca ttatcccttg
     2701 caggtgtttc agcgattttc tcattatcat gcgcacttat tgtatcttta gctccggatt
     2761 tttcgctcag attggcaagg gctttattcc atgggctcga gatctcaggg atagcagctc
     2821 caacgataaa tgatatactc gcaggtttgg gcggtgttac cataatcgct atcctgtctg
     2881 gatggttgtt tgctttattc tacaacttta ttacaacaaa gagatagata tgcaactaaa
     2941 ggaaaaagat actgtcctat cgttgatcct ctttccagcg gcgtattact cactcccttt
     3001 atcaatagat ggcgcaggca tcgcagtcga agttgttttt tcatctttgt ctttcttttt
     3061 ccttcaatcg gccagatgag cttggatgtc attgaaatta tgggcaagtg ttacatcaac
     3121 aatgaatggt gcgattgctc aagacttggg ttcatacccc gcagctcggc tagcggttgg
     3181 gattttttat tggcacttgc ctatctatga tctctaggac acaaagggcc ggaaaaccat
     3241 tcgtcaaaaa cgtttttgag gtgcttgaga aatccaacaa tagaaattag gacattctga
     3301 cccatctcct atgctaaatc atggagcttg gtgaatgcat aaatgtctgt ccaaaaaaag
     3361 cattgcccta tcggttcagc tggtgacctt cagtgttccc caatgtccgt ttgccctatg
     3421 tcctggcttg gtgcagtaat actcaaatga tcctgccttg tcagcggcaa actcgacagc
     3481 aacttgctcg ttaagaggag tctcggtata agcattgaac tcatctatct tgaagtcatg
     3541 ggttcctttg gtgtttgtta tctttatcct tacggtatct cctttgttca cagttatctc
     3601 atttggcgaa aacttaggat agtacttttc atctattatt tccgtatacg actccatgac
     3661 aaattccttg acctgcggtt tttggctgca cccgctgagg attatagatc ctaacagcaa
     3721 tattgcaagt aatgctttca ttttaaggcc ctcttatctt attatcagtt tgcctttcat
     3781 ctggctgtgc ccgctgccgc acatcacatt gcagtaccat gtaaactcac cggttttgtc
     3841 agcagtaaac tctattactt catttcctgc aagccccagt tcaggtatcc ttatgccatg
     3901 cgtcgtgtca gtattgtcta tcaccagctt taccttgtcg cccttgctca cagtgacgac
     3961 ctcaggactg tatccgaacc tgaatgccga gaccttgatt tccgttgtat cctttacagc
     4021 gctggctgta ggggcgtctg ctccaagcaa accaaatatc cttattgccc caaaagctac
     4081 cgcagccact atcaggatta tgattgccat ctcaactatc ttttcttttt ccatgtgatt
     4141 cacctctcag gcgagggtag cattgtcata tataaatgtt atgccaatgt aaccttaatg
     4201 tcatcagaaa gtaatttaaa taagggtgct taaaagaagg aaaatacgga ggttattact
     4261 atgagcacga taaatgaggt attgtcgata agtgcatcgg ttgccctttt gctggcagta
     4321 tattttagct acaagctgtc aagagagaca aagcacgaaa gatattggct gttgctttcc
     4381 ttgggcctgt ttatttttgc ggtccatcac tggttcatga taatattcca gcttggaaat
     4441 atcccatttt ttattctgga gctgcttgaa tatcttacat cgatagtggc agggatattg
     4501 atagcatact cgacctacgg cctttacaag tctatgaggg ctgtcaatca gagagtgcga
     4561 tgataccaaa atccattctt ttttattgga gtaaaggcgc tgatgtaagg aggaagatat
     4621 tgaagatact tagcgactgc gaaaagcagg ccaagccatg ttttctaaac atcgctgcaa
     4681 aaaagctttc cctcactcat gttgctgtaa aaaagcacat tgacctcatg gtagaagaag
     4741 gatatattga gcccattaat ccaaaaggaa agccagtttt tctaaaactt acccctttag
     4801 gaaaaggcgt gattaaggag cttgaaaaaa gatagaaatc atctgctttc accttcaatc
     4861 cactcctcac actctttgtt tgccgatgcc tctatcctta ttatgcaagg cacctcataa
     4921 gaatgcacat tccttacctc attttgtatt ttgtcccagt tagcgctggg cgcttttgca
     4981 tcgatcacaa attcagttct tttctcgatc tttcccttcc accaatacat gctcgatatg
     5041 ggatgcatgt tggagcaagc tatcaacctt tttttaagca ggtgctctga aacagattta
     5101 gcctcctctt catccttgca ggttatatgc actctgatca taaatttcca aatatcacta
     5161 gatttatata tcttatgaga gttcgaaaag gttatgcata acaaatacat ccattcaggc
     5221 atcttaggta tattcattta taatgaacat tttaacgtat tggaaaagca tttattcaat
     5281 aataatgaac aataccttaa cggaaaaaag cttgaagaag agatgcataa aaaatactcc
     5341 aacctaaaaa agccagaggg aaaagagcta aaaaagatac ttgagctttt tatgggcaag
     5401 gagtattttc ctgactttag aaaaaacgcc ttgagcatta caaggcttga gataaagcat
     5461 tcagtaaaag acgacctgct tataatgcag gcgataaact gcgtagacga aacatcaaag
     5521 acaataaaca tccttatcaa gcgcttaaga gaatggtatg cgatatataa tccagaactg
     5581 gagcacgaga tagaaaatca ggaaaaattt gtagagcttg ttctcacaaa aagcaaaaaa
     5641 gagctgttga aggagatcca ggtagacgaa aaagcgtcta tgggcgcaga tttggccaag
     5701 gaagacatcg agcccatcaa agccttggca aaaaaagcag aggagttgtt cgatctaaag
     5761 tcagtcgagg aagcctatct tgaagcgaca atgaaaaaag tgtgccccaa tctcttggca
     5821 gttgcaggtt cgactattgg tgcaaggctt atggagcagg caggcgggct caaaaacctg
     5881 atgctgtttc ccgcttccac cgtccaattg ttaggtgcag aaaaagcctt gttccgacat
     5941 atgaaaacaa aagctagagc tccaaaatac ggctttatcc acgagcatcc aatcatatcc
     6001 agagcaaaaa aaagcatgca tggaaagctc gcaagaagcc ttgcactcaa gataagcatc
     6061 gcagtcaaga tagactattt tcatggaaaa ttcatagggg ataagcttct taaagagctt
     6121 gaagatataa gatgaaagca acaagcatct ttgaagtgta tgctgatgaa aaaggcaggc
     6181 cttacacagt taatgccgct ccaggtatcc aggtctatga tgaaaagctt gtaacactta
     6241 aaggggttga atatcgggaa tggaatccaa ggaaaagcaa gcttgcagcc tccataatga
     6301 aaggcagccc gaatatcttc atgagaaagg gggatgttat cctctacctt ggctgctcta
     6361 ctggaacgac agtctcccat gtttcagaca tagtaggcga tgaaggattc atattcgcct
     6421 tagactttgc gccaagggtt atgagggaga tggtatttac agcagagaaa aggaaaaaca
     6481 tagcccctct cctggctgac gcaaacaagc cagaggctta tgcaggaaaa gtgaccatgg
     6541 cagatgtcat ataccaggac atagcgcaaa gaagccaggt agaaattttc ctgaagaacg
     6601 taaatctgtt cctgaaaaaa ggaggctatt gccttcttgc tgtcaaggca agaagtattg
     6661 acgtcacaaa aaagccaaga gtgatattca gcgatatacg ccagaggctg gaaaaagaat
     6721 tgaccattat tgattaccgc acattagagc catttcagat ggaccactgc atgtttatct
     6781 gtaaaaacaa caaataaatt ctcttatatc cttgactcta ataaacctca taaacccatt
     6841 cattaagaaa aatggtgtcc accatgcaat atttcaatcc tactctagtt caattctaac
     6901 agtttcttat ctgccattac ctgcgcttgt ttcaccattt caatcctact ctagttcaat
     6961 tctaacaaag atatccatag atacaaccac caaaaactgc taatttcaat cctactctag
     7021 ttcaattcta actaatttac ggaacataca gcatcatggg tgaaaaagaa tttcaatcct
     7081 actctagttc aattctaact gatgttattc atcgggggct gttcttttct ttccaatttc
     7141 aatcctactc tagttcaatt ctaaccatat ccggcgatgc caaagaggaa gagaataagt
     7201 tatttcaatc ctactctagt tcaattctaa catccttcta agggttgcac agatcactac
     7261 agtagatatt tcaatcctac tctagttcaa ttctaacccg ataccgacga aggacgttag
     7321 ccttttgttg tatatttcaa tcctactcta gttcaattct aacatcttca caaaattaca
     7381 tgagaacata ctgctcttta tttcaatcct actctagttc aattctaacc cgccaaattt
     7441 ggctctaaat gaagaaacaa gcccaaattt aaattattta tgcatttgcc cagtgaagca
     7501 aggatgcctt gtttataaag gctgggctaa agaatgttgg ataattcgtt cctttctctt
     7561 ccaatcactt ccttctcaaa ggaatgttta tccctgaact tgaatatcag gatagaatca
     7621 gtttctttgt caacccattg attaagcttc ttttttactt ccaggaaatc agcatcccct
     7681 aactcccctt caaatactga attttgtatc caattcatat atcttttcaa gtatttgtat
     7741 attttattta ctttgcttac atcaacatca tagactaata aataatacat tttaccacca
     7801 aattacaaat gggctatagg ttttatctcc gattagatgc tttaccagct tatatgattc
     7861 aagcctgatt aaccttctgt gggaaaccag gcgctttagc tcaggatgct caatagtaag
     7921 cttgagcctg tcatcaaatt cttttaggac gatccttctt ccatcctcat ttagcataca
     7981 actatttaag tccttgcgga aatgattatc cttgagttca ctcttattaa aaagcttaag
     8041 catagccctc tgaacaatca gcggtttaaa catctcgcta atatccaagg ccaaagaaaa
     8101 tcttctttca aagggctcat gaagatagct aatggtcgga ttaagctggg tatgataaat
     8161 ctcgcttaag gttgtggtgt aaagaaggga attcaggaag ctgataacgc agtttacctc
     8221 gttatgcggg ggcctcctcg tcctaacttc aaattcttcc ttgagtattt cttcataaga
     8281 tgaatagtaa tcattccatg cgcagccttc tacattcatc agctcaggaa ttgtgctgca
     8341 actatccagc ttgggaacac ctttagccag cttgtccata atatcaccca cttcctttga
     8401 gtgtttccgg tagtgctcca atatccttat caggttctgg atagtgcctg aaatgaagga
     8461 tttggctaaa aacagccgct taccagagtc tgtataatag ttaacctggt ttacaagaag
     8521 cgaccctgaa acaaggcttt cttttggata gaaactgcct gaataatatc catagtaatt
     8581 gaagaaatgc aggattattc cctgctttgc aagaaacaca agcagttttg agtttataga
     8641 aacttcgcca aggcagtaaa tggtgtctat ggagtttacg gggatcgctt tcctgatctt
     8701 ttcattctcg aaccagacag tgctatcttt ccttttgatt gttccagact gtgtgatata
     8761 gtagttttgc ttttgcattt tagctgaaac atagctcata atagctgcag agcctgcagt
     8821 atttgtttct tttggcctca ggtacctgtt cctggcaaat aagctctgcc tgccttattg
     8881 cgttgtttag caaatcttca tttgcttggg ttaactcaac ttcttcaagg cggttattct
     8941 ctttatactt gatcgttcca gtggtaaata ttcctttttc ctttaatttt agcaggtagt
     9001 aaaggacctg atattttgct gcctctggag cggtgttctt cttttttagc tcaaatactt
     9061 ttccctctct caatgaatca atcttgacgt tgtcaatctg caagctttct ttctctatat
     9121 cctgatggtg gacctttcct atcttgacca gctcagattc atgctcacaa gttattttct
     9181 ttgaaaaaag ccatagcttt cttttgcagg tgaagaaata gttgatgtga gtggctgtga
     9241 gggccatata acctggaaat tgagtgcagt ataagttttt taccattaaa tttaaataga
     9301 gtatcaactt tttgtttata gttatcaaca aaatgaaaac agatcaaaaa ataatcaagg
     9361 cgctgattaa gagtccggat actaacacaa taagcgcctt atctggggaa attgggataa
     9421 gcagacaggg tgttttcaag gtagttaaaa gacttgaact tgaaagattg ttgaggttat
     9481 ctaaagtagg aaaaggcagg acaagcatat ttgtaattaa actaaattgg cagaatccca
     9541 ttcttgaaaa acaagctgca atcatcttgc ttgatgaggc gcagaaaaac caaaggtgga
     9601 taacaaattt tatcgaatta gaaaaaaatg tcaagtttac cctcatattt ggcagcgtcc
     9661 tgacatcacc taaagaagca aaggatatag acttgataaa tgtagcttct gactttatta
     9721 caatcgacaa gatcatcaat gctacccaaa aaacacaaac aaaaaaaata catgcgctga
     9781 atcttacaga agcagagatt agggaggaac tggcaaaagg caatcctgcc ttgacagagg
     9841 ctattaagaa aggggttgtc ttatttgggc atgaaaactt catcaaattt atacgagggc
     9901 taaatggaag ttaaggcatg ctttttaaca gcactcaagg atgagggcaa aggcagaaaa
     9961 cacaaagggc tgttaataaa agccccggat gaaaaaatgg cgggagagta cattactaaa
    10021 gtaaaaagaa atcttgaact tt
//
LOCUS       DASRUK010000031        22054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1704889, whole genome shotgun sequence.
ACCESSION   DASRUK010000031 DASRUK010000000
VERSION     DASRUK010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 22054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22054
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704889"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..4742
                     /locus_tag="VFF28_01390"
     CDS             24..4742
                     /locus_tag="VFF28_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44315.1"
                     /translation="MDENSIHLKIDNQEPAFTKAYAQGKIEIRGALNLSIGRHNTSIS
                     AKDAVGNSNTVEWKFEVFVPVICLVNACDTDKGKWCAQGIWTEQEYCSHCGLADPVCE
                     MGCDDGNCSIPSNQWCSDGAWAISDYCAHCGSLDSDCQSGCTQGTCDIEKNKWCDNNL
                     WVEESYCLKCGAKDAQCGINPPQLAGFTVDSIPSISGEYFSRQNLQIGWRYTGKSNIT
                     NIRLNNTPVPEWKTKLVSPDSIGYSYQLQNLANDRSYAFSVESSNDGFASSRHSFVFS
                     IDATAPTITLDDSLDTYTSNPTVKIFGTYTEKNLKQIAVNGMALDSSEIGKTTFGKNV
                     ILPAGKTNITGSIEDLAGSKDEDIVEIEIIGGELPLSIDALPDYTKESRITLRGKTLP
                     GLAVKITLNNIRLSAASNPQGNFSMELTLKEGNNEIIVNATDAAERSTQIIRHIILDS
                     TGPEIVERSPERNEKGNFGQIAIKLRDASGIDTASIELRVNDGIVTHTYEEKEQTIYY
                     NIEGLPEMGYTITLSAKDVLENRMATSWSFTLNKQAPLINITYPTSKGFVGESYPDII
                     LDIKDYDMVNEVSLTRGEQRVEMVKFSVFKYRVREKLALQEEYHLTVKASKNNNMAEA
                     HRYFRVDLNSPVITIDDSRIPSPTNKWSLDIYGTFEEENLESISVNGIKVEPIDIFEH
                     TYIARNIPLGISDTKITASIKDMVGRTGNYSTIAKVDRILPALTITNPPNSAASVVGN
                     NKVDIIGTAEANSMLLINGIEQGILADGIIHLTYYGLVKGRNEINISVVDEAGNVNSE
                     LRAAYYDAEGPAITNIMPNKDNPQNKNTFAINANINDHSRISYVELLINDTPEFRYIV
                     PSAELTSNQFRLEQAFTPSKNSSYTITITARDVFGNSNIAKETFIFDADIPSRPLFNL
                     EGKIINSSQPNLIFTYEEEVNITSLNLSTITNSTSDLKTYSFIAANLLDGKHTLAIAA
                     SRLVGRQYSFPWVFDFTVDTHKPGISISNTVKALSSSLLITINGTCSDNLAKYENISI
                     IIYEGGFPFTGVSCVNGMYSKDIIVGGAGVRTIYVVANDTAGNSNSASKQILVDTGEP
                     FINIASITGGMIIKEGDVYRTNSGQATITGNVADQNNEKFEANLNGLSTPLTWDAGSG
                     NFEATVSLQGNAGKEAANIVVLTATDKVGIESKKSISIIYDQAGPNITSFYPIHAYNL
                     TPTIQIMLNENATSCSLKYKTGVGPTETYDMNTRNNKAYYLSLLVKLRNITESQEINI
                     SCVDMLSNINSKKYYIFVDTINPTILEHDLIYEHKLDFGSDSTGKSYLIKKLNSDGQY
                     APIALMVQPSEDARCRYYGSKVGNFAAYEYRLGKTALLSDNVIMLADNTIYDFNIVCE
                     DRAGRASETKSIRATVNTSYPVNAPHISLISPWAIAGAVSNSWSLWFNGSIISYLDSQ
                     IDTSSLTILNITRILARDSQGRFSELVNVKNDGEFDFIIFANNTAGLSTTKSGSVLID
                     TTGPGACIRIGNNVTCIPSQGRAAAGIYDYDKDGLPDWFEYKYWSCITCANNNDDTDG
                     DGYSNLQEYSYGRNPIDRLL"
     gene            4739..7447
                     /locus_tag="VFF28_01395"
     CDS             4739..7447
                     /locus_tag="VFF28_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009391718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HZX44316.1"
                     /translation="MKHAAVIYGVCLLFFLTPAFADTRPDIRITFYERVNESTVTINL
                     TDVAGTRYDTVLIDSFGIESDNPTFIYKPAGELTEGRYHIKAYARDMHNVWGPQVEQD
                     FMATISDLNINIVRPRFGVTNTTTFDLEIGTDEDRNAVCKYGWGYSFYDFSLNLGGRH
                     IKNNISITSSSNIQIRCVDKYNKTYQIDTQIKLDETYPSIRIEAEDVVQMPLLTPLRV
                     YADEEVICRYGYNPSSYSLMTPFEGYREDNLGDYIRNPVQTLGMLQNGKTYTFSVICK
                     DLAGKASNMAITSFKVDLSAEPTVAIRSPAGVLLSRTFYVNASTNKDASCKYSRNQTY
                     TDFGEYGKSHLSNLIGPLVDGSYTYNVDCVFSTESGPITKRSSTSFVIDSTKPNMTYV
                     NITNTADNDSNRIYSDDEICAEWDAVDSESAISSYKYYVFLDKTQDQLIDQGDENPES
                     SKEYCIDTSLNNSEKYYLKVQAQNAAGLWSENKSSISVLVDTSLSPPGCDNGRRDGTE
                     TDKDCGGSCDGCVLGLSCRLNSDCETNFCNASSKCAKASCGDQIKNGDESDVDCGGSC
                     KKCSQEKSCKTGKDCESNNCQDKKCEEVLDKCENNRWDESIETDIDCGGKCPECSIGA
                     KCISDLDCGQTAECKDKKCLLKATDKDKDGITDDNDNCADVPNKDQSDADKDGIGDEC
                     DLDSDNDNMPDSFEEKYFDCKTCAKPDEDLDGDGLLNLEEYQNTTDPTNPDTDGDGFS
                     DKEEITQGSDPLDPTSTPDKGGGFFIWVLIFLLIGALGAGGFYGYKLLTARKPFIPPS
                     RGEKGQNIFEKKPLPKLPIRPPIIIPHPPVQKPAIKPPIALPPAAKPKPAPAKTKKEP
                     KKEIDVFKKLATISRAERIDQAKGQASSMSNKELNEKINKLKQQLK"
     gene            7471..7971
                     /locus_tag="VFF28_01400"
     CDS             7471..7971
                     /locus_tag="VFF28_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44317.1"
                     /translation="MNKRKKGQISGQIFAYVTTIIIFSFILIYGYKALTDFREKEEHL
                     SFIKFKKDITSAVERIAPEHSSVEREEFYLGGDYTQVCFLQNYQSPGNLDLLNHDMDK
                     IVKDSYLANSTNNVFVYAGTFKEPFYIGRINTTEDGYLCIDAVKGKIKVELQGKGDHS
                     LISRWG"
     gene            7990..8427
                     /locus_tag="VFF28_01405"
     CDS             7990..8427
                     /locus_tag="VFF28_01405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein bridging factor aMBF1"
                     /protein_id="HZX44318.1"
                     /translation="MCGKDTELYKTDIEGSIMNLCKECAKFGKVISPPTTMPRYAVKK
                     QAAVQEERTISVIEDFGERIKKKREESALSQEEFAKKLNEKLSIIHKLETGQFVPPIR
                     LALKIEKCLGIKILEQQKIESEKVTQTKGEHFTIGDYIKVRKK"
     gene            complement(8428..9735)
                     /locus_tag="VFF28_01410"
     CDS             complement(8428..9735)
                     /locus_tag="VFF28_01410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZX44319.1"
                     /translation="MAIQTFSLFVDHDIWWDSSVYIGMGKYIFSLGESGLWEPSRPLL
                     WPLALGFFWKIGISPIFAGKILLILISGACLSVVYLMAARIYNDKTALISALILAFSS
                     TFFLYNSVLQTEIPSLLFVLLGVFFFIDKKYGLSGLMIGIAFMTRFFTLLAFFGLAAS
                     ILVKVFVKKVSIKQAVLFFVLFLVPLLPYLMLSLFLYGDFIYPFTLQANMTQTTGWVF
                     NQPFWFYWVNLLKENILLPLALVTLISAVKKRKDGFIPSMLLFTFVPFNLMDHKEMRL
                     ILIALPFLAILAAEGLVIISAFFRKYSNKMITLVVLFSIGLALPSLSFNSYDGPFGLY
                     HRYLDNNNISGVLWISNPAYIVNTDYRAELLYYPTFDNSKMESLDSRIWNSTIMINTC
                     DTPCPPWESECEDNKKEFIGRLKEKYGLLAYDRVWDCDMYIFG"
     gene            10147..10950
                     /locus_tag="VFF28_01415"
     CDS             10147..10950
                     /locus_tag="VFF28_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZX44320.1"
                     /translation="MIVKEEFLSKLRRYFSLNLYEVKIWTALLSRGVSTAGELSDIAD
                     VPRSRSYDVLESLEKKGFVVMKLGKPIKYLAVPPHEVLERVKKNVKEDAKDRIDRLEE
                     LKSTDVIDELNSLHSQGVELVEPADLSGSLRGRHNLYNHLELTIRNAENNVTLMTTSQ
                     GFIRKVEGLKPTFEKIKKRGVRIRIAAPITKEAMNAVKDLKNIAEIRHTDSKARFCIV
                     DGKELIFMVLDDSDVHPTYDVGVWVNTPFFAQALDSLFDVAWKNMKPIK"
     gene            11173..11982
                     /locus_tag="VFF28_01420"
     CDS             11173..11982
                     /locus_tag="VFF28_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009007847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HZX44321.1"
                     /translation="MQDNKLKEIAKEVRKDILTATCKAQSGHPGGSLSATDILVCLYF
                     SKMKHNPKKPSDPDRDRFILSKGHAAPALYSCLARSGYFPIKELETLRQLGSRLQGHP
                     ERGKLPGIEASTGPLGQGLSFANGVALAGKLDKKTYKVYCMIGDGESQEGMIWEAAMT
                     SAHYKLDNLCVILDHNRLQIDGDVEKVKGIEPIVDKFKSFNFEVIEIDGHNYDEILEA
                     LNKADKIKGKPTMIIANTIKGKGVACMENKAEWHGKACKPEELDNLCRSIA"
     gene            11984..12913
                     /locus_tag="VFF28_01425"
     CDS             11984..12913
                     /locus_tag="VFF28_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HZX44322.1"
                     /translation="MEQKAEATRDGFAKGIVKAGENKDVVVLDADLAESTRSIKFKEK
                     FPDRFFDMGIAEMDMVGTAAGLAASGKIPFACSFAVFSTGRAYEAMRLAAISKLNVKI
                     VGSHAGLATGEDGATHQSIDDISLTRTLPGMTVIQPVDSLQAEKATIALAEHQGIAYL
                     RTGRAKLPILTKESDKFQIGKGEILAEGKDLAIIATGSMVSIALEAAQELSRLKLSAK
                     VINIHTIKPLDAALILKAAKETGRIITAEDHSIIGGLGSAVSEVLSENYPVKLKRIGL
                     NDTFGESGKPESLYQKYGLTKENLIKAAKALRG"
     gene            12918..13706
                     /locus_tag="VFF28_01430"
     CDS             12918..13706
                     /locus_tag="VFF28_01430"
                     /EC_number="2.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate
                     synthase"
                     /protein_id="HZX44323.1"
                     /translation="MIGKSIRMQRIMDRNTGKTVIIPMDHGVTVGPISGLENMKETID
                     RIADGGANAIVLHKGMVNVGHRSGGKDVGLMIHLSASTSLGPDTNNKVLVCTVEEALK
                     LGADAVSIHVNIGAEDEAEMLKDMGKVASRCHEWGVPLLAMMYPRGKKIKDEKDVEVV
                     KLAARVGAELGADIVKTNYTGDIASFKQVVEGCPVPVIIAGGSKGNDADCLKMIEDAM
                     KAGAAGVAMGRNSFQHENPAKFVKAVCEVVHQGKSSKDACKLLR"
     gene            13717..14709
                     /locus_tag="VFF28_01435"
     CDS             13717..14709
                     /locus_tag="VFF28_01435"
                     /EC_number="1.4.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007547237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase II"
                     /protein_id="HZX44324.1"
                     /translation="MKKLWVNVPKWDKSIVTTALESNADAVLVPKGFSKNVKDLGLIK
                     TISEDGDIKLGKDVVIITINSKQDEINALKESKSKIVIVRTKDWTVIPLENLIAQTKG
                     IIAEVSNSTEAKTALETLEKGVDGVLLNTTNLSEIKKTAALIKSSTEKILLVKAQITS
                     IKAVGTGDRVCIDTCTNMKTGQGMLVGDSSSGMFLVHSESVENPYVEQRPFRINASAV
                     HAYIKVPSGKTKYLSELKTGTETLIVDAQGNTEIAIVGRTKIEKRPLMLVEAVANGKR
                     ISLILQNAETIRLVSPQGKPVSIVKLKKGDTVLAYTEDAGRHFGIKIKETIIEK"
     gene            14726..16126
                     /gene="aroE"
                     /locus_tag="VFF28_01440"
     CDS             14726..16126
                     /gene="aroE"
                     /locus_tag="VFF28_01440"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HZX44325.1"
                     /translation="MIAVPIVSDSIKKALADIKKAERVADIIEVRLDYIRDINKINLT
                     KLLAASKQKIIITDRKNRLDLINESVRLGADFIDLDISIGSKLIREVTKQSKAKLIVS
                     FHNFKETNRKELTAKYKEIKKLRPGIIKIVSFANSINDNLVIFDLINQAKKDKVDIIA
                     ICMGEKGEISRILAPIYGSFLTFGSLESGKESAPGQIPAEILRQVYRVDKLKNPKIFG
                     LVGNPVSHSRGIYFHNEQFKKVNKNSIYLNFLVDDPKAFIKGFKPIISGLSITIPFKT
                     EIIPHLDKLDPAAKEIGAVNTVVKQKDKLVGYNTDMLGAIDAIKSKTSIAGKKILMIG
                     AGGVARAVGYGIVKEKGDLMITNRTEEKALELARQLNCSSTKLDNSHQLGKFDIIINC
                     TSIGMFPKITQTPIPKPLLNLLSKKAAVVFDTVYTPKLTKLLKDAGKLGISVVGGNDM
                     FIAQALEQQKLFQKVK"
     gene            16126..17415
                     /gene="aroA"
                     /locus_tag="VFF28_01445"
     CDS             16126..17415
                     /gene="aroA"
                     /locus_tag="VFF28_01445"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HZX44326.1"
                     /translation="MKLLVEKTEKLSGEITIPGSKSHTIRAVTIASLAKGVSKIRNPL
                     KSDDTMASVNAWKLLGAKINTDDANEWIIEGVDHKPKAPGSALNMLNSGTSLRIISGI
                     IAALCDFEVELDGDDSLRTRPMQPLLKAYNELGAEALSISNNGYCPIKIKGKMTGGST
                     EISGLSSQFVTSLLFACPLLEEDTKIKVLKPNEIPYIKMTLRWLDEQGIRYQASEDFT
                     GFTVFGSQKYKPFEKTIPADWSSAAFPLVAGAITNSDITIKGLDLKDTQGDKAIIDYL
                     KKMDADIKLDGNEVHIKGKPLKGCQLDLNSTPDALPALSVLGAFAEGTTTLYNVPQAR
                     VKETDRIKVMAEELGKMDVDIKEREEGLIIHKSALKGNKVRGHNDHRVVMALSLAGLI
                     AEGKTEITSAESIGVTYPGFIESMQSLGAKIKLLGGK"
     gene            17417..18568
                     /gene="aroC"
                     /locus_tag="VFF28_01450"
     CDS             17417..18568
                     /gene="aroC"
                     /locus_tag="VFF28_01450"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HZX44327.1"
                     /translation="MMGNTFGRLFRVTTVGESYGKGKGSGLAVIVDGVPPGLKITRED
                     IQKELDERKPGVGKLNSPRKETDQCDIFAGIGQDGVTTGAPIGIIIYNVDTQKVHIEQ
                     YRAYRDIFRPGHATYPFFLKYGEAQDWCGAGRSSGRETVGRVAGGAVAKFILAQEGIE
                     IVSCTKECAGIKAKEMPFEEIKANRRKNEINCPDLVAAKKMEEEVLKIKGEGDTAGGI
                     IELIARGVPPGLGEPVFDKLSATIAHALASIGAVKGVEFGAGFNVGRMLGSENNDQPY
                     LKNGKVKFRTNNSGGLLGGFSNGEDIVVRIAVKPTPTISKEQTSINMKAMKEEKLSPI
                     TRRDPTLVARLWAVAESMMAIAILDSLYMARAYDGMAKLDKKWMELKQK"
     gene            18585..19439
                     /locus_tag="VFF28_01455"
     CDS             18585..19439
                     /locus_tag="VFF28_01455"
                     /inference="COORDINATES: protein motif:HMM:NF042869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HZX44328.1"
                     /translation="MEAKTIGIIGGTGQMGQWLKRFFEKNDCKVLIAGRKTKLTPEEL
                     TQRSDVIILAVPISAVTAAVKQICAHIRPDSLLMDIASLKKDPVKAMLENVSCAVIGT
                     HPVFGPSVQSIKNQTVVICPARPKQWLSWLTDLFENNGALVRITTPEKHDKMMSIIQG
                     VNHFSTICMAHTLKKLGIDVEESLHYTSPIYKVRMDMVGRLLAQDPYLYADIEMLNPE
                     NKKAMEEYISSARELLGIIKRKDRDEFVKYFEEGSDFLGNFKKEAVEYSDYIIEQLVK
                     KGSLKDPK"
     gene            19436..20245
                     /gene="pheA"
                     /locus_tag="VFF28_01460"
     CDS             19436..20245
                     /gene="pheA"
                     /locus_tag="VFF28_01460"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HZX44329.1"
                     /translation="MKIATLGPKGTFSHEAALKRDIKAELVFKNTVWDIFESVSKGEV
                     SEGVAPIENSVSGSIGLTLDALMEFDLNIVGEIILPVKHNLVSQASIESIKILYVHPA
                     AYEQCERFIRNNLPNAKIIQTSSNAASAELVANSKDTSTGAIVPNEAIKIYNLKTIKK
                     DIQDSKFNVTRFIIISRKKAKKTSRDRTSIAIYPQVDKPGLLYSLLGEFAKRKVNLTK
                     IESRPSKGKLGDYIFFIDVLGNSEDASVKEAFKKIEENFFLKVLGSYRKEY"
     gene            20259..21779
                     /locus_tag="VFF28_01465"
     CDS             20259..21779
                     /locus_tag="VFF28_01465"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF003210.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HZX44330.1"
                     /translation="MENNKLIQSLHAYERKVLSVLDKKSTVSEIAQKSSLQEIEVMRG
                     LQWLENKQLVKTEEEIKELADLDKNGKRYVKEGLPEKRFLQAVGENTPISEIKKKASL
                     QDEELSICLGVLRSKAAIAIKKEKELTVSILPNGKTLLGKESLEEQFIKKSFPLDTST
                     LKDEEKFALESLRKRKEIVKVEKKIIKKAELTDLGKQVQKKGVTAEHYEDRLTPSMLK
                     ENSWKNKKFRHYDVKINVPQIFGGRKQHYAHFLDNVRLKFMSLGFKEMFGPLVETEFW
                     DMDALFMPQFHSARDIHSAYYIKDPQHGKIDDKLLEKVKASHEIGKGTLSRGWQYKFD
                     VNKTKQLILRTQGTALSARMLASKELKVPGKYFGIARCFRPDVVDATHAADFNQVEGI
                     VVEEGLNFQHLKGLLKLFAEEFAQTDQIKITPGYFPFTEPSADLFAKHPELGWIELGG
                     SGIFRPELVEPLLGRQIPVLAWGLGVDRLAMFKLGINDIRELFSHNLKFLRSAKLI"
     gene            21781..>22054
                     /locus_tag="VFF28_01470"
     CDS             21781..>22054
                     /locus_tag="VFF28_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HZX44331.1"
                     /translation="MPTITFSLKDLQNLLGKKLTIEELEELVSYGKGELDNYDKETNE
                     VSVDFGDTNLPYLWSVEGIARYFKGILGIEKGIPKIEIAKSGCKVIV"
BASE COUNT         7593 a         4304 c         4984 g         5173 t
ORIGIN      
        1 ctttgattgc tgataactat gctgtggatg aaaatagcat ccatctaaag atagacaacc
       61 aggaaccagc attcaccaag gcatacgccc agggcaaaat tgagataagg ggcgctttaa
      121 atctcagtat tgggcggcac aatacatcta taagcgcaaa agatgcagtt ggaaacagca
      181 ataccgtcga gtggaagttt gaagtttttg taccggtaat ctgccttgta aatgcgtgcg
      241 acacagacaa aggcaaatgg tgcgcccaag gaatctggac agagcaggaa tactgcagcc
      301 attgcggact tgctgaccct gtttgcgaga tgggctgtga tgatggaaac tgcagtatac
      361 catcaaacca atggtgctct gatggagcat gggcaatcag cgattattgc gcacattgcg
      421 gcagccttga cagcgactgc caatcaggat gcactcaagg aacatgtgac attgagaaaa
      481 acaaatggtg tgacaataac ctctgggtag aggagagcta ctgcctgaaa tgtggagcca
      541 aagacgctca gtgcggcatc aatccgcccc aactggcagg gttcaccgta gactcaatcc
      601 cttcaatatc aggagaatat ttcagcaggc agaacctgca gataggatgg aggtatacag
      661 gcaaaagcaa tataaccaat atccgcttaa acaatacacc tgtacctgag tggaaaacaa
      721 agctcgtttc acccgacagc ataggctatt cctatcagct gcagaatttg gcaaacgaca
      781 gaagctatgc gttctctgtc gaatcatcaa acgacggttt tgccagcagc aggcattcct
      841 ttgttttttc aatcgatgca actgctccaa caataaccct agatgattcc ctagacactt
      901 atacaagcaa tccaactgta aaaatattcg gaacctatac ggagaaaaac ctcaaacaga
      961 tagctgtgaa tggcatggcg cttgacagct ctgaaatagg caagacaacc tttggcaaaa
     1021 atgtgatcct tcctgcaggt aagaccaata tcactggctc aatagaagat cttgcaggaa
     1081 gcaaggacga ggatatagta gagatagaga tcatcggtgg agagctccca ttatcaatag
     1141 atgcactgcc ggattacaca aaagagagca ggatcaccct caggggaaag acgcttccgg
     1201 gccttgctgt caagataaca cttaacaaca taagattatc agccgcaagc aatcctcagg
     1261 gaaatttctc aatggagctg acattaaaag aagggaacaa tgaaattatt gtcaatgcta
     1321 cagatgctgc agaaaggtca acacagataa tcaggcatat catcttagac agcacggggc
     1381 cagaaatcgt ggaaagatcc ccagaaagga acgagaaagg aaactttggg cagatagcaa
     1441 taaaacttag ggatgcaagc ggcattgata cggcctccat agagctaagg gtcaacgatg
     1501 gaattgtaac gcatacctat gaagagaaag aacaaacaat ctactataat atcgagggcc
     1561 tgcctgaaat gggatataca ataaccttga gcgctaaaga tgtgctggaa aacaggatgg
     1621 ctacatcctg gtcgtttaca ctcaataaac aggcgccttt gataaatatc acatatccaa
     1681 caagcaaagg ctttgtaggt gagagctatc ctgatattat ccttgatatc aaggattatg
     1741 atatggtcaa tgaggtcagc ttaaccaggg gtgagcaaag agtagagatg gtaaagttct
     1801 cagtcttcaa atatagggtt agagagaagc ttgctttgca ggaagagtat catctgacag
     1861 taaaagcctc taaaaacaac aacatggctg aagctcacag atactttaga gtagatctaa
     1921 actctcctgt catcacaatt gatgatagca ggataccttc accgaccaac aagtggtcac
     1981 ttgatatcta tggaacattt gaagaggaaa accttgaaag cataagtgta aatggaatca
     2041 aagttgagcc tatcgatatc tttgagcaca cctacatcgc aagaaacatc ccacttggca
     2101 tcagcgatac aaagatcact gcaagcataa aggacatggt cggaaggacc ggtaactatt
     2161 cgaccatcgc caaggtagat agaatattgc ctgcacttac cataacaaac cctccaaatt
     2221 cagcggcatc tgtcgtcgga aacaacaagg tagatatcat agggacagca gaggcaaatt
     2281 caatgctgct catcaatggc atcgagcagg ggattcttgc agacggcatc atccatctga
     2341 cttattatgg gctggtgaaa ggcagaaatg agataaatat cagtgttgtt gatgaggcag
     2401 gcaatgtaaa ttctgagcta agagcggcct actatgatgc agaagggccg gctattacaa
     2461 atataatgcc aaacaaagac aaccctcaga acaagaacac atttgcaatc aatgcaaata
     2521 tcaatgatca ttccaggatt tcgtatgttg agcttttgat aaatgatacg cccgagttta
     2581 gatatattgt tccctcagcc gagcttacat caaatcagtt tagacttgaa caggcattca
     2641 ctccatcgaa gaacagctct tataccataa ctatcactgc aagagatgta tttggcaact
     2701 caaacattgc aaaggagaca ttcatatttg acgcagacat tccgtcaagg cctttattca
     2761 accttgaagg aaagatcatc aattcttcac agcctaactt aatattcact tatgaagaag
     2821 aagtaaatat cacttcgctt aatctgagca caatcaccaa ctcaacttct gacctcaaga
     2881 cctacagctt tatcgcggca aatcttttag atggaaaaca tacgcttgcg atagctgcgt
     2941 caaggcttgt tggcaggcaa tatagcttcc catgggtatt tgactttact gtggatactc
     3001 acaagccagg gatctctatt tcaaacacgg ttaaggcttt aagttcaagc cttctaataa
     3061 caataaatgg aacatgctcc gataacttgg cgaaatatga aaacattagt ataattatat
     3121 atgaaggagg cttcccgttt actggtgtca gctgcgtgaa tggcatgtat tctaaagata
     3181 tcattgtcgg cggagccgga gttagaacaa tatatgttgt ggcaaacgat actgcaggca
     3241 actcaaactc cgccagcaaa caaatacttg tagacacagg agagcctttt atcaacatcg
     3301 caagcataac cggcggcatg ataatcaagg agggggatgt atacaggacc aattcagggc
     3361 aggcaactat cactggaaat gtcgctgacc aaaacaacga aaaattcgag gccaacttaa
     3421 acgggctatc gactcccctc acatgggatg cggggtctgg aaattttgag gcaactgtga
     3481 gcttgcaagg caatgcgggc aaagaggcgg caaatatagt ggtactgaca gctactgaca
     3541 aagtaggaat cgagagcaaa aaaagcatat ctatcatcta tgaccaagct gggcctaata
     3601 tcacctcatt ttacccaatc catgcataca atcttacccc caccatacaa ataatgctga
     3661 atgaaaacgc aacttcatgc tctctaaaat acaagacagg agtgggaccc actgaaacat
     3721 atgatatgaa taccaggaac aataaagcat actatctatc cttgcttgtc aagctaagga
     3781 acattacaga atcccaggaa atcaatatct cgtgcgttga catgctttca aacataaatt
     3841 ccaaaaaata ttatatcttt gtagatacca taaatccaac aatactagag catgacctta
     3901 tatatgagca taaacttgac tttggcagtg acagtaccgg aaagagctat cttatcaaaa
     3961 agctgaacag cgacggtcaa tatgcaccta tagccttgat ggtgcagcca tcagaggatg
     4021 caagatgcag atactacggt tcgaaggtag gcaattttgc tgcctatgaa tacaggcttg
     4081 gtaaaacagc actcctctct gacaatgtta taatgcttgc tgacaataca atctatgatt
     4141 ttaacatagt atgtgaagac agggcaggca gagcctctga gacaaagagc ataagggcaa
     4201 cagtaaacac atcgtatccg gttaatgccc ctcacatatc attgatcagt ccatgggcaa
     4261 ttgctggagc tgtttctaac tcatggtcat tatggttcaa tggctctata atctcctatc
     4321 ttgattcgca gatagacact tcaagcttga ctatccttaa cattaccaga atcctagcaa
     4381 gagacagtca aggaaggttt tctgagcttg ttaatgtaaa gaatgatgga gaatttgact
     4441 ttatcatatt tgcaaataat acagcagggc tatccactac aaagagcggc tctgtgctca
     4501 tagatacaac agggccaggg gcatgcatac gcatcggcaa caatgttact tgcatcccta
     4561 gccagggaag agccgccgct ggaatctacg actatgacaa agacggcctt cctgactggt
     4621 ttgaatacaa atactggagc tgcataacct gcgcaaacaa taatgatgat actgatgggg
     4681 atggatacag caacctacaa gaatacagct atgggagaaa tccaatagac cgtctattat
     4741 gaaacatgca gctgtcatat atggagtgtg ccttctattc tttttaacac ctgcatttgc
     4801 agatacaagg ccagatatca gaataacatt ttatgagagg gtaaatgaaa gcacagtcac
     4861 tatcaacctg acagacgtcg cggggaccag atacgatact gtgctaatag attcttttgg
     4921 cattgaaagc gataacccta cctttatcta taagccagca ggcgaactta ctgaaggacg
     4981 ctatcatatc aaggcttatg ccagggatat gcacaacgtc tggggcccgc aagtcgagca
     5041 ggatttcatg gccactatca gcgacctaaa tataaacatc gtcaggccaa ggtttggcgt
     5101 gacaaacacg acaacctttg acctagagat aggcacagat gaagatagaa atgcagtgtg
     5161 caagtatggg tggggctatt ccttttatga ttttagcctt aacttggggg gccgccatat
     5221 aaagaacaat ataagcatca cttcctcttc aaacatccaa atacggtgtg tcgacaaata
     5281 taacaaaact taccagatcg atacccaaat aaagcttgat gaaacatacc cctcgataag
     5341 aatagaggcc gaggatgttg tacagatgcc tcttttaacc ccacttaggg tctatgcgga
     5401 tgaagaggta atctgcaggt atggatataa cccctcttcg tactcgctca tgacgccttt
     5461 cgagggatat cgcgaagata atctaggcga ttatataaga aacccagtgc aaacactagg
     5521 catgcttcaa aatggcaaaa cctatacctt ctctgtaatc tgtaaagacc tggctggaaa
     5581 agcatctaac atggccatca catcttttaa ggtagatctg tcagcagagc ctactgttgc
     5641 tatccgtagc ccagcaggag tactgcttag cagaactttc tatgtgaacg caagcacaaa
     5701 caaagatgcc tcctgcaaat attccagaaa ccagacatat actgattttg gggagtacgg
     5761 taaaagtcac ctaagcaatc tcatcggacc cttggtagat ggaagctaca cctataatgt
     5821 cgactgcgtt ttcagtactg aatcaggacc gataaccaaa cgctcaagca caagctttgt
     5881 gatagacagc acaaaaccaa atatgactta cgtcaatata accaacacag cggacaacga
     5941 ctcaaatagg atatactcag atgatgagat atgcgccgag tgggatgcag tcgacagcga
     6001 gagtgcaatc tcttcctaca aatactacgt attcctggac aaaacccaag atcagttgat
     6061 agatcagggg gatgaaaatc ctgaaagcag caaggaatac tgcatagata cctcgctaaa
     6121 caactctgaa aaatattatc tgaaggtaca ggcgcaaaat gcagctgggc tatggagcga
     6181 aaacaagagc agcatctctg tccttgtgga taccagcctc agccctcctg gatgcgacaa
     6241 tggcaggagg gatggcactg agactgacaa agactgcggg gggagctgtg atggatgcgt
     6301 ccttgggcta agctgcaggc tcaactcgga ctgtgaaacc aatttctgca atgcaagcag
     6361 caagtgcgcc aaagccagct gcggcgacca aataaaaaac ggcgatgaat cagatgtaga
     6421 ctgcggaggg tcatgcaaaa aatgctcgca ggagaaatca tgcaagacag gcaaggattg
     6481 tgaatcgaac aattgccaag acaaaaaatg cgaagaagtc cttgataagt gtgagaacaa
     6541 cagatgggat gaaagcatcg agacagatat tgactgcgga ggcaagtgcc ctgagtgttc
     6601 gataggcgct aaatgcatct ctgaccttga ctgcggacaa acggcagagt gcaaagacaa
     6661 aaaatgcctg ctaaaggcga ccgacaagga caaagatgga ataacagatg ataacgacaa
     6721 ctgcgcagat gtgccaaata aggaccaatc tgatgctgac aaagacggga taggtgatga
     6781 atgcgaccta gattccgaca atgacaatat gccagactcc tttgaagaaa agtattttga
     6841 ctgcaaaaca tgcgcaaaac ctgatgaaga cctcgatggg gacggccttt tgaaccttga
     6901 ggaatatcag aacacgactg atcctaccaa tccagacact gacggggatg gtttttccga
     6961 caaggaagag atcactcaag gcagcgatcc tttggatccg acttccaccc ctgacaaagg
     7021 aggcggcttt tttatctggg tattaatctt tttattgata ggcgcactgg gggcaggagg
     7081 attttatggg tataaactat tgactgcaag aaaaccgttc atccctccct caagaggaga
     7141 aaaggggcag aacatctttg agaagaagcc tttacctaag ctacctatca ggcctccaat
     7201 aattatacct catccccctg ttcaaaagcc agctataaaa cctcctatag ctttgcctcc
     7261 ggcggcaaaa ccgaagccag ctccagcaaa aaccaagaaa gagccgaaaa aggaaattga
     7321 tgtgttcaag aaactggcga ctatatccag ggcggaaaga atagaccagg caaaaggaca
     7381 agccagcagc atgagcaaca aagagctgaa cgaaaaaata aataagctaa aacagcaact
     7441 taaataatca ttacatattc ccaccattct atgaacaaac gcaagaaggg ccagatttca
     7501 gggcagattt ttgcctatgt tacgactatc atcatattct cattcatact aatatatggc
     7561 tataaggcgc ttacagattt cagggaaaag gaagaacact taagctttat caagttcaaa
     7621 aaagacataa cctctgcggt agagagaata gccccggaac attcgagcgt cgaaagggaa
     7681 gaattctatc ttggcggcga ctatactcag gtttgctttt tgcaaaacta tcaaagccca
     7741 ggtaatcttg acctactaaa ccacgatatg gataaaatcg tcaaggatag ctatcttgca
     7801 aacagcacta ataatgtatt tgtatatgcc ggcaccttta aggagccatt ttatatagga
     7861 agaataaaca ccactgaaga tggatactta tgcatagatg ctgtgaaagg aaagataaag
     7921 gtggagctcc aaggcaaggg ggaccattca ctcatcagca gatgggggtg agcaatcatc
     7981 aactgcgaca tgtgcggcaa ggatactgaa ctttataaaa ccgacataga gggttctata
     8041 atgaacctgt gcaaggaatg tgcaaaattt ggcaaggtca tatcgccgcc aaccactatg
     8101 cctcgctatg ctgttaagaa gcaggcggcg gtgcaggaag agcggactat ctctgtcatc
     8161 gaagattttg gcgaaagaat caagaaaaag cgagaagaat ctgcattgag ccaggaagag
     8221 tttgcaaaga aacttaacga aaagctttca atcatccaca aactcgaaac cgggcagttt
     8281 gtcccgccta taaggcttgc acttaagata gaaaaatgcc ttgggatcaa aattctcgag
     8341 cagcaaaaga ttgaaagtga aaaggtcact caaacaaaag gggagcactt caccataggt
     8401 gattacatca aggtcagaaa gaaatgatca gccgaatata tacatatcgc agtcccatac
     8461 tctatcatag gcaagcaggc catatttttc tttgagacgc cctataaact cttttttgtt
     8521 atcttcacac tcgctttccc aaggagggca tggggtgtca catgtgttta tcataatagt
     8581 ggaattccat atcctcgaat caaggctctc catctttgaa ttatcgaacg taggataata
     8641 taagagttca gccctataat ccgtgtttac aatgtatgca ggattggata tccacagaac
     8701 cccgcttata ttgttgttat ccaggtatct atgatacagt ccaaatggcc catcataact
     8761 gttgaagctc aaagaaggca gagctaaccc aatagaaaac agcactacta gggtaatcat
     8821 cttatttgaa tacttcctaa aaaaggcact tatgatgacc aggccctctg cagcaaggat
     8881 ggctaaaaag ggcaaggcaa tcaggatcaa cctcatctct ttatggtcca taagattgaa
     8941 agggacaaat gtaaacagca gcatagacgg gataaatccg tctttcctct tttttaccgc
     9001 tgagatgagg gtgactaagg ccaagggcag caatatgttt tctttaagca ggttcaccca
     9061 gtagaaccaa aaaggctggt tgaacaccca tcctgtggtt tgggtcatat tggcttggag
     9121 agtgaacgga tagatgaagt ctccgtagag gaataggctc agcatcaaat aaggaagcaa
     9181 tggaaccaag aacaagacga aaaacaggac tgcttgcttt atgctcacct tttttacaaa
     9241 caccttaaca agaatactgg ctgcaaggcc aaaaaaggcc aggagagtaa agaaacgcgt
     9301 catgaaagca atccctatca tcaatccaga aagcccatac tttttgtcga tgaagaaaaa
     9361 gactcctaaa agcacgaaaa ggagcgaagg gatctctgtc tgaagcacag aattatataa
     9421 aaaaaaggtt gatgaaaatg caagtatcag ggctgatatc agggctgtct tatcgttata
     9481 tatccgagca gccatcaagt atacgaccga taggcaggca ccactaatga ggataagtaa
     9541 aatctttcct gcaaatatgg ggcttattcc tatcttccaa aaaaatccca aggccagggg
     9601 ccagagcaac ggccttgaag gttcccacag gccagactct cccaacgaga agatgtactt
     9661 gcccatgcct atataaactg acgaatccca ccatatgtca tggtctacaa acaaagagaa
     9721 tgtttgtata gccataaaaa taactataac tcctgctgct atcaatctaa acttcggctt
     9781 tttatttgtt gtaaataccc caccgcttgc ggtgactggg ttattgttat ggctcgccac
     9841 gttgagggta tttacaacac taaaaatacc ccgccccttg gggcggttgg gatttatttt
     9901 ttacttacga tcaaggttaa ataattttgt cttgctaaca tatattttct ttttacataa
     9961 tccttatata ttaccatacc cccagttatt aaagttgttt taactaaaga gttaaaatta
    10021 actgtaccta acgaaaagta aacgtactta gccgaggtaa aaaagcgtaa catatattaa
    10081 gtagtaccaa ctttttaccc caatgagaag tgaaaatact tctctcaaaa aagggggtga
    10141 atgtagatga tagtaaaaga agaattttta agcaaactga ggagatattt ctcattaaac
    10201 ctctatgagg ttaagatatg gacagcgcta ttgtctaggg gagtttcaac agcaggagag
    10261 ctttctgaca ttgcagatgt tccaagatcc aggagttacg atgttttaga aagccttgaa
    10321 aaaaaaggat ttgtggtgat gaagcttgga aagcctataa aatatttggc agttcctcct
    10381 catgaagttc ttgaaagagt aaaaaagaac gtaaaggagg atgcaaagga caggattgac
    10441 aggcttgaag agttgaaaag caccgatgtc atagatgagt taaattcgct tcattcccag
    10501 ggtgttgagc ttgttgagcc agctgacctt agcggttcgt taaggggaag gcataatctc
    10561 tacaatcatt tggagctgac gataaggaat gccgagaata acgtaacatt gatgactaca
    10621 agccagggct ttatcagaaa ggtcgagggc ttaaagccaa ctttcgagaa gataaaaaaa
    10681 aggggtgtaa ggataaggat tgcagctcct ataaccaagg aagcgatgaa tgcagtaaag
    10741 gatcttaaga acatcgcaga gataaggcac acggattcta aagcaaggtt ttgcatcgtt
    10801 gacggcaaag agcttatctt catggtgctt gacgactcag atgtccatcc gacatacgat
    10861 gttggtgttt gggtaaacac tccattcttt gctcaggcgc tcgacagctt gtttgacgtc
    10921 gcttggaaga atatgaagcc gattaaataa ggataaattt tttattttat ttataagccc
    10981 caagtcaaag aaaaatttat atacaagcgt ttgttacctt atcaaaatga atcctatagg
    11041 gaagaagcaa acaagcaggt tttttctagg ttcttcctat ttttacttta gatagtctct
    11101 aggccataaa cttagaagac actcatccct tatcctcaaa aaatttaaaa accaataggt
    11161 gaaaaagcac atatgcaaga caacaagctt aaggaaatcg caaaggaagt aagaaaagat
    11221 atactgacag ctacttgtaa agcccaatct ggtcaccccg gcggctctct ttcagcaaca
    11281 gacattttgg tctgcctata cttcagtaag atgaagcata accccaagaa gccaagtgat
    11341 ccagacaggg acaggttcat actaagcaaa ggccatgcag cgccagctct ttactcctgc
    11401 ctggcaagat caggttattt ccccattaaa gaactggaaa cattaaggca gctgggatca
    11461 aggcttcaag gtcatcctga aagaggaaag cttcctggaa tcgaagcatc cactggtcct
    11521 cttggccagg gtttaagttt tgcaaacggt gtcgcactcg caggaaagct tgacaaaaaa
    11581 acatacaagg tttactgcat gataggagac ggcgagtctc aggaaggtat gatatgggag
    11641 gcagccatga cctcagccca ttacaaactg gacaatttat gcgtcatcct tgaccataat
    11701 cgcttgcaga tagatggcga tgtggaaaaa gtaaaaggta tagagccaat agtagacaag
    11761 ttcaaatcct tcaattttga agttatagag atagatggtc acaactacga tgaaatattg
    11821 gaagccttaa acaaggcaga caagataaaa ggaaagccca ctatgataat cgcaaacaca
    11881 ataaaaggaa aaggagttgc atgcatggaa aataaggctg aatggcatgg caaagcatgc
    11941 aagccagaag agcttgacaa tttatgcagg agcattgcct aaaatggaac aaaaagctga
    12001 agctacaagg gatggatttg caaaaggcat agttaaagca ggagaaaata aagatgtcgt
    12061 tgtcttggat gcagatcttg cagaatcaac ccgttcaata aagttcaagg aaaaatttcc
    12121 agacagattt tttgatatgg gcattgctga gatggacatg gtagggacag cagcaggcct
    12181 tgcagcctca gggaagatac cgtttgcatg ctcttttgca gtattttcaa caggaagggc
    12241 atatgaagca atgcgccttg cagcgatctc taaactcaac gtaaaaatag ttggcagtca
    12301 tgcaggcttg gcaactggcg aagatggagc aacccatcaa tctattgatg atatatcgct
    12361 tacaagaacg cttccaggaa tgacagttat ccagcctgtt gattctcttc aggcagaaaa
    12421 agcgacgatc gctttagcag aacaccaagg catagcttat ttgaggactg gaagggcaaa
    12481 gctcccaata cttacaaaag aatcagacaa attccagata ggcaaaggcg agatacttgc
    12541 agaaggaaaa gatcttgcga taatcgccac cggatccatg gtttctatcg cacttgaggc
    12601 agcacaagag ctaagtagat taaagctttc agcaaaagtg atcaatatcc acacaataaa
    12661 gcctttagat gcagcattaa ttttaaaggc agcaaaagag acaggaagaa taattacagc
    12721 agaggaccat agcataattg gaggcctggg ctcagctgtt tcagaagtac tatcagaaaa
    12781 ttatcctgta aaactcaagc gcataggtct taatgataca ttcggtgagt ctggaaagcc
    12841 agaaagctta taccaaaaat acggtctgac aaaagaaaat ttgatcaaag ctgccaaagc
    12901 tttaaggggg taaaaaaatg atcggaaaaa gtatcaggat gcaaaggatt atggacagaa
    12961 atacaggaaa gacagtgatt attcctatgg atcacggtgt gactgtaggc cctatcagcg
    13021 gccttgaaaa tatgaaagag accattgaca ggatagcaga tggtggggca aacgcaatag
    13081 tccttcacaa aggcatggtc aatgtcggcc atcgctcagg aggaaaagac gtaggcctta
    13141 tgatccatct ctcagcatca acaagccttg gccctgacac aaacaacaaa gtcctggtat
    13201 gcactgtaga agaggcgcta aagcttggtg cagatgcagt ttccatccat gtaaatattg
    13261 gagcagaaga cgaagctgag atgctaaagg atatgggaaa agtggcaagc agatgccatg
    13321 aatggggagt tccgcttctt gctatgatgt atccaagagg aaaaaagata aaagatgaaa
    13381 aagacgttga ggtagtaaag cttgcagcaa gggttggcgc agagcttggc gcagatattg
    13441 tcaagactaa ctataccgga gacattgcct ctttcaagca ggttgttgaa ggctgccctg
    13501 ttccggtgat tatcgcaggt ggctcaaaag gaaatgatgc tgattgcctg aagatgatcg
    13561 aagacgctat gaaagcagga gcagcaggag ttgccatggg aagaaattcc ttccagcacg
    13621 aaaaccctgc taagttcgta aaggcagtct gtgaagtggt gcatcaggga aaatcatcca
    13681 aagatgcttg taagctatta agatgatagg tgcattatga aaaaactatg ggtaaatgtt
    13741 ccgaagtggg ataaaagcat tgtcacaaca gcgcttgaaa gcaatgcaga tgcagtcctt
    13801 gttccaaaag gattttccaa aaatgtaaaa gaccttggcc tgataaaaac aataagcgaa
    13861 gatggagaca taaagcttgg aaaagatgta gtcataatca ccatcaacag caagcaggat
    13921 gagataaatg cattgaagga atctaagtca aagatagtga tagtaaggac aaaagattgg
    13981 acagtgatcc cgcttgaaaa cttgatagcc cagacgaaag ggataatcgc agaagtctct
    14041 aattcaacag aggcaaaaac agctctggaa acccttgaaa aaggggtcga cggtgtcctt
    14101 ctaaacacaa caaacctcag cgagataaaa aaaacagcag cattgataaa atcaagcact
    14161 gaaaaaatac tgctcgtaaa agcccagata acaagcataa aagcagtcgg aacaggcgat
    14221 agggtctgca tagacacttg cactaatatg aagacaggcc agggcatgct tgtaggagat
    14281 tcaagctctg ggatgtttct cgttcattct gaatctgttg aaaaccccta tgttgagcaa
    14341 aggcccttta ggataaatgc atctgctgtt catgcttaca taaaagttcc aagcggaaaa
    14401 acaaaatact tatcagagct aaagacaggt acagaaactt tgatagtaga tgcccaggga
    14461 aacacagaga tagcgatagt cggcaggact aagatagaaa aaaggccgct tatgttggta
    14521 gaagccgttg caaatggtaa aaggatatct ttgatacttc aaaatgcaga gactatccgt
    14581 ttagttagtc cgcaaggaaa gccagtatct atagtcaagc tcaaaaaagg agacactgtg
    14641 cttgcatata cagaagatgc aggcaggcat tttgggataa agataaagga aacgatcata
    14701 gagaaatagg ggtgtaaaaa aaataatgat tgctgttccg atagtatctg acagtatcaa
    14761 aaaggctctt gctgatataa aaaaagcaga aagagttgca gatataatag aggtaaggct
    14821 tgactatatc cgagatatca ataaaatcaa tctaactaaa ttattggcag ccagtaaaca
    14881 aaagataatt atcacagaca gaaaaaacag gttggatctg ataaatgagt cagttaggct
    14941 tggtgctgat tttattgacc tggatatatc cataggcagt aaacttatta gggaagttac
    15001 taaacaaagc aaagcaaaat tgattgtttc tttccataat tttaaggaaa caaacagaaa
    15061 agaattaaca gccaagtaca aagaaataaa aaaattaagg ccgggcataa tcaagatagt
    15121 ctcattcgca aactcgatca atgataatct agtgatcttt gacttgatca accaggcaaa
    15181 aaaagacaag gtagatatta ttgcgatttg catgggagaa aaaggcgaga tctcaaggat
    15241 acttgctccg atatatggct cttttcttac ctttggctct ctggagtcgg gaaaagaatc
    15301 agctccaggc cagatacctg cagagatcct aaggcaggtt tatagggtgg acaaattaaa
    15361 aaatccaaaa atctttggat tggtaggtaa cccggtcagc catagcaggg gcatatattt
    15421 tcataatgag caatttaaga aagtaaacaa aaattctatc taccttaact tcttagtaga
    15481 tgaccctaag gcattcataa agggattcaa gcctataatc tcagggctaa gcatcactat
    15541 cccatttaag actgaaatta tccctcactt ggacaagctt gaccctgcag caaaagagat
    15601 aggcgcagta aacactgttg taaagcaaaa agataaacta gtaggatata atacagatat
    15661 gcttggcgca attgatgcga tcaagagcaa aacaagcata gcaggaaaaa aaatattgat
    15721 gataggtgca ggaggagtgg caagagcagt aggatacggc atcgtaaaag agaaaggcga
    15781 tcttatgatt acaaacagga cagaagaaaa agcacttgaa ttagcaaggc agctaaactg
    15841 tagtagcaca aagctagaca attctcacca attaggcaaa tttgatatta ttatcaattg
    15901 cacttctatt gggatgtttc caaaaataac tcaaactcct atccctaaac cattgctaaa
    15961 ccttttgtca aaaaaagccg cagttgtttt tgacacagtt tatactccca aattaacgaa
    16021 acttttaaaa gatgccggaa aattagggat aagtgtagta gggggcaatg atatgttcat
    16081 cgctcaggca ttagagcagc aaaagttatt ccagaaggtg aaatgatgaa attattggta
    16141 gaaaagacag aaaaactaag cggagagatt actatacctg gctcaaagtc tcacacgatc
    16201 agggcagtga ccattgcatc tcttgcaaaa ggcgtttcaa agatcagaaa tccattgaag
    16261 tcagatgata caatggcttc agtaaatgca tggaaacttc ttggcgcaaa aataaatact
    16321 gacgatgcaa acgagtggat aattgaagga gttgaccata agccaaaagc tcctggctca
    16381 gcccttaaca tgttaaactc aggcacatct ttgcggatta tctctggaat tattgcagcg
    16441 ctatgcgatt ttgaagtgga gctggatggc gacgattcac tgaggacaag gcccatgcag
    16501 cccttgctaa aagcttacaa cgaattaggc gcagaagcat tgtctattag caataacggc
    16561 tattgcccga taaagataaa aggaaagatg acaggcggct caacagagat atcgggcctt
    16621 tcatcccagt ttgtgacaag ccttctcttt gcctgccctt tattagagga agacacaaag
    16681 ataaaagtcc taaagccaaa cgagattcct tacataaaga tgacgcttag atggcttgat
    16741 gagcagggca taagatatca ggcttcagaa gactttactg gatttacagt tttcggcagc
    16801 caaaagtaca agccctttga aaaaacaata cctgcagatt ggagttcagc agcatttcct
    16861 cttgttgcag gagcgataac caattcagat attactataa aaggccttga cttaaaagac
    16921 actcagggag acaaagccat cattgactac ctaaaaaaga tggatgcaga cataaagctg
    16981 gatggaaacg aggtacatat caaaggaaag cctttgaaag gatgccagct ggacttgaac
    17041 agcacaccag atgcacttcc ggcattgtca gtacttggtg catttgcaga agggacaacc
    17101 actttataca atgtccctca agcaagggta aaagagacag ataggattaa agtcatggcc
    17161 gaagaattag gaaagatgga tgttgatatc aaggaaagag aagaaggatt gataattcac
    17221 aaaagcgctc ttaaaggaaa taaagttagg ggccacaatg accacagggt tgtaatggcg
    17281 ctaagccttg caggccttat tgcagaagga aaaacagaga ttacatcagc agaatccata
    17341 ggcgtcacat atcctggatt cattgaatct atgcagtcgc taggagcaaa gatcaaattg
    17401 cttggaggaa aataggatga tgggaaatac ctttggcagg ctttttaggg taactacagt
    17461 cggcgagtcg tatggaaaag gaaaaggctc aggccttgca gtgatagtag atggagtccc
    17521 gccaggcctc aagatcacaa gagaagatat tcaaaaagag ctggatgaga ggaagccagg
    17581 tgttggaaaa ttgaacagcc caagaaaaga aactgatcag tgtgatattt ttgcaggcat
    17641 tggccaggat ggtgttacca caggagcgcc gattgggatc ataatctata atgttgacac
    17701 tcaaaaagtg catattgaac agtatcgtgc atacagagac atattcaggc caggccatgc
    17761 tacttatcct ttcttcctaa aatatggaga ggcccaggat tggtgcggag caggaagatc
    17821 atctggcagg gaaacagtcg gaagagtcgc aggaggagca gttgcaaaat ttatactcgc
    17881 tcaggaagga atagagatag tcagctgcac aaaagaatgc gcaggaataa aagcaaaaga
    17941 gatgcctttt gaagagatca aagcaaacag gagaaaaaat gagatcaact gccctgacct
    18001 tgttgcagca aaaaagatgg aagaagaagt gttgaagata aagggcgaag gagacacagc
    18061 agggggcatt attgagctta ttgcaagagg tgttcctcca ggccttggcg agccggtctt
    18121 tgacaagctg tcagcgacga tagctcatgc tctggcatcg atcggcgcag taaaaggtgt
    18181 tgagtttggc gcaggattta acgtcggccg catgttaggc tctgagaata atgatcagcc
    18241 ttatttaaaa aatggaaagg ttaaatttag gacaaataat tcaggaggct tgcttggcgg
    18301 tttctctaat ggtgaagata ttgtggtcag gatagcagta aaaccgactc caacgatctc
    18361 aaaagagcag acatctataa acatgaaggc gatgaaagag gaaaaattaa gccctataac
    18421 ccgcagagac ccgacccttg ttgcaaggct ttgggcagtg gcagaatcga tgatggcaat
    18481 agcgatactt gattcattat acatggcaag agcctatgat ggtatggcaa agctggataa
    18541 gaagtggatg gaattaaaac agaaatagat gataaaataa caaaatggaa gcaaaaacaa
    18601 tagggattat cggcggaaca ggccagatgg gccagtggct taagagattc tttgaaaaga
    18661 atgactgcaa ggttctgatt gctggaagaa agacaaaact tacaccagag gaactcactc
    18721 aaagatcaga tgttatcatc cttgctgttc cgataagcgc agtaactgcc gcagttaagc
    18781 agatatgtgc gcacataagg ccagactctc ttcttatgga cattgcatca ttaaaaaaag
    18841 acccagtcaa agccatgctt gagaatgtga gttgtgcagt tatcggcaca catcctgtct
    18901 ttggtccctc agtgcaatct attaagaatc agacagttgt gatctgcccc gcaaggccaa
    18961 agcaatggct ttcatggctt actgaccttt ttgaaaataa tggggcattg gtgcgcataa
    19021 caactcctga aaagcatgac aagatgatgt ctatcatcca aggagtaaac cacttctcga
    19081 cgatttgtat ggcccacact cttaagaaac taggcattga tgttgaagaa agcctgcatt
    19141 atacttcgcc gatctataag gtgaggatgg atatggttgg aaggcttctt gcccaggacc
    19201 cctaccttta tgcagacatt gagatgctta atcctgaaaa taaaaaagcc atggaagaat
    19261 atatcagttc agcaagggag cttcttggaa taatcaagag aaaggacagg gatgaatttg
    19321 taaaatattt tgaagaaggc tcggattttc ttggaaactt taagaaagaa gctgttgaat
    19381 attcagatta cattattgag cagctggtaa agaagggaag cttgaaggac ccaaaatgaa
    19441 aatagccacg cttgggccaa aaggcacatt cagccatgaa gcagcattaa aaagagacat
    19501 caaggctgaa ctggtattca aaaatacagt atgggatatc tttgaatcag tcagcaaagg
    19561 cgaagttagt gaaggtgtgg ctcctataga aaactcagtc tcaggatcaa tcggccttac
    19621 ccttgatgca ttgatggaat ttgatctaaa tattgtcgga gagataatcc ttccggtaaa
    19681 gcataatctt gtcagccagg cgagtatcga aagcataaaa atattatacg ttcatccggc
    19741 agcatatgag caatgcgaga gattcatcag aaataacctc ccaaatgcaa aaataatcca
    19801 gacaagctca aatgcagcat ctgcggaatt agttgcaaat tcaaaagaca catccacagg
    19861 agcgatagtc ccaaacgaag cgataaagat atacaaccta aaaacgataa aaaaagatat
    19921 ccaggatagc aagtttaatg ttactaggtt tatcatcata agcagaaaaa aagcaaaaaa
    19981 aacatcaagg gataggacct cgattgcgat ctatcctcag gtggacaagc caggccttct
    20041 atactctttg cttggagaat ttgcaaaaag aaaagttaat cttacaaaga tagaatcaag
    20101 gccttccaag ggcaagctgg gagactatat cttctttata gatgttttag gaaacagcga
    20161 ggatgcaagc gtaaaggaag cattcaagaa gatagaagaa aatttctttt taaaagtgct
    20221 cggctcctac cgcaaggaat actagcatgg gatactagat ggaaaataac aagctgattc
    20281 agtcattgca cgcctatgaa agaaaggtgc tttcagttct tgataaaaaa agcactgtct
    20341 cagagattgc gcaaaaaagc agccttcagg agatagaagt catgaggggg cttcagtggc
    20401 ttgaaaataa gcagcttgta aaaacagagg aagagataaa agagcttgca gaccttgata
    20461 aaaatggtaa gcgttatgtc aaggaaggtt tgcctgaaaa aagatttctg caggcagtag
    20521 gcgaaaatac gcctatatcg gaaataaaaa agaaagcaag cttgcaggat gaagaattaa
    20581 gcatctgtct tggagtccta agaagcaaag cagccatagc gataaaaaaa gaaaaagagc
    20641 ttacagtatc cattctaccc aacggtaaaa ctctgcttgg taaagaaagc cttgaggagc
    20701 aattcataaa gaagagcttc ccgttagaca cctcaaccct caaggatgag gaaaaatttg
    20761 cccttgaaag tttaaggaag agaaaagaga ttgtcaaggt cgaaaaaaag ataataaaaa
    20821 aagcagagct tactgatctc ggtaaacagg ttcaaaaaaa aggagtgaca gcagagcact
    20881 atgaagacag gctcactcca tccatgctta aagaaaactc atggaagaat aaaaagttca
    20941 ggcactatga tgttaagatc aacgttcctc agatattcgg aggaagaaag cagcattatg
    21001 ctcacttcct tgataatgtt cgcttaaaat ttatgtctct cggttttaag gagatgtttg
    21061 gcccattggt agagacagaa ttttgggaca tggatgcatt gttcatgcct cagttccatt
    21121 cagcaagaga catccattct gcttattata taaaagaccc gcagcatggc aagatagatg
    21181 ataaactcct tgaaaaagta aaagcttcgc atgagatagg aaaagggaca ctaagcagag
    21241 gctggcaata taagtttgac gtaaacaaaa caaaacagct tatcctaagg acacaaggga
    21301 ctgcactttc agcaaggatg cttgcaagta aagaactaaa ggttccggga aagtattttg
    21361 gcattgcaag atgtttcagg ccagatgtcg ttgatgcaac acatgcagca gactttaatc
    21421 aggttgaagg gattgtagtt gaagaaggcc tgaacttcca gcatttaaaa ggcctattaa
    21481 agctttttgc agaggaattt gcccagactg atcagataaa gataaccccc ggttattttc
    21541 cgttcacaga gccttcagca gatctgtttg caaaacatcc tgagcttgga tggatcgagc
    21601 ttggtggttc agggatattc aggccagagc ttgttgaacc tttgcttgga aggcagatac
    21661 ctgtgctggc atggggtctt ggagtagaca ggcttgcgat gttcaagctt ggcatcaatg
    21721 atatcagaga attattctcc cataatctaa aattcctgag gagcgcaaaa ctgatctaaa
    21781 atgccaacaa taaccttttc acttaaggac ctgcagaatt tgttaggaaa aaagctaaca
    21841 atagaagagc ttgaagagct tgtttcctac ggtaaaggag agctggataa ttatgataaa
    21901 gagacaaatg aagtctctgt tgattttgga gacacaaacc tcccatacct ttggtcagtg
    21961 gaagggattg caagatattt caaggggatt ctgggcatag aaaaaggaat acctaagatt
    22021 gagatagcaa aatcaggctg caaggtgatt gtta
//
LOCUS       DASRUK010000032        14257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_367488, whole genome shotgun sequence.
ACCESSION   DASRUK010000032 DASRUK010000000
VERSION     DASRUK010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 14257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14257
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367488"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..1372
                     /locus_tag="VFF28_01475"
     CDS             278..1372
                     /locus_tag="VFF28_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44332.1"
                     /translation="MVYYSFPYSHNITWPPNFTVKYGPTPVETWNPGIIQQDFFEQQQ
                     AIHTLPREYWPGPCERIGSFGATETYFKFALWKMACETDHSLEEIVPQKTLLYVQKSG
                     ADPVFQNIGGLTMADHYGHFNFRDVGKLETRIFDIKKNAEDFMKSPEGRRFALDNSDL
                     LKDAPEIDEICVKVNYPGRASMLQLSEDRARLTGNYNIKKYEEDVANALGIGVDYQMR
                     STFAEEFHHLIRRDVIKSTSHGIRIEARVRELLIDMYGKMEEATKDAGGKKAYGRIKA
                     HLEWELKNIRKLYEKAYSEEDGELESTLDAKKQYNKGETPQGLEEVLDDVSAETCAAE
                     ATISNGDNGRTSGAESDGPSGDGGGEGGGE"
     gene            complement(1374..2144)
                     /locus_tag="VFF28_01480"
     CDS             complement(1374..2144)
                     /locus_tag="VFF28_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44333.1"
                     /translation="MISSLRSVLDKIKKNKSLFCLIILLQLGIIVLLTLASFFTIIPA
                     MDKAKAAVDYYDKINLTENPGMFDYLGNDPAIVFQSYRRVVEYFKLLALIAIPGYILL
                     TSLIWSLSDRVLFDKKRFLSYFAKCAFSLAIFFLLAYGVLFEKLLSALAEENMIALTG
                     PVILLAIMGYLFFGMLCLISTKKNFLKEGIVLGFKKIHKITMIYIACLTAIALLCLAI
                     FISIEASLLILILLIIVLMALIAFLRLFLIEWVNELAK"
     gene            complement(2141..8248)
                     /locus_tag="VFF28_01485"
     CDS             complement(2141..8248)
                     /locus_tag="VFF28_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44334.1"
                     /translation="MKTRQMVCIFLIFLVITLPITLAESLVITNVRVEQVTQNSARVK
                     WETNTNAKGSVNYGATKELGKNRQSNAFDDVHDIALTGLDVNAPYFFEVKSEEVTDNN
                     NGNYYQFTTLPNQPVFIDATLPSYLSKKTISVSGKTIRLAEVNLFVNADEPRKLLAGD
                     DGSFGFPSIALIEGENTIKLTAKSQGQSAEKIYKVILDTKDPILNISSIPDIVGKEKF
                     SINGTADEPVNISYSVRSSTTKPEMPPKVQSLRNTSVKPNKVDLSWDKVDIKDFEQYI
                     VYRNDTPLGIGSDSGYNDYSDVLANSGNTYSYRVAAMNKFGLIGEKSDSIMIRTPEGG
                     RKDLPQENVKIEIGSKKTIEVQKDFSLEFDLGKEDGYYTININAIDRAKNQWLLEKTI
                     LLDTKDPEIKIISPKANANIFQNYADMVDISGKSEPGARIYLYMYRTPFSYFNTTWDA
                     YGAPDYISQLDEAQLRANCRAEIDGKEQCKTHADYEAVADENGNFVIENVDLTSIWAG
                     AVSVEELPTGEPYYDFVKFKETHDSLQSRLFFVAVDPAKRKGFLQADYTITTCWSPLL
                     TWDATPLVEYQSPTFLSVERLKEGTEAIYFYLNFTYHGQGKDAKVTNLNIEKACGKGY
                     LESLDRFNHSCKLIRSCTEKLSPNGKTAYVACGLGRLEGVESWHDSDWESFIQAVKEE
                     MAFPFKLRLDYYDEDINNKTKQYGHYQICTEVSYQLDAVKINPREILPDWLLYDFTDS
                     LNKSIGVLNDWILKIRRVLEWAALACMVTFFTKFVLQIYRRMVCAYDSVYKDVQKLGG
                     TSGTNEECNKCIQDSKSASKKYEEGSKEGAKGKKDFQDDLSDACLKVCYPSCASVWGA
                     EESLYQTYRWACDRVFGHPTPSKWTESEDDIALFEKAASGSSCATDQSVRGRPLRAVD
                     CQATAKKYPNPRDFDRDDKCLEVSSTEKGMKYMTLYKIDSPIDAGNDLYSVSVVGGDE
                     KIGVDFVIKQNEKNYLTHMEQTCEEICSQDITGKRSTAGFKVGKKDAQIITPGSEPKG
                     VTQSDKQGEKLIMASGCAKTNECLSYASNENAKFQVEGQSTPVEIKSAMPMGYTSDCW
                     QADQVSDNPDRRVECCCLNSKAGAFPGYYQPADVESKDGGPEASSYEDMRWSYRYYKM
                     KYLNKVFNPLRYMQGRDLSACFGQDLWFYGRGKILTIDPSKQHIAAFQCVAISQILAR
                     LSMIRNLMVALENCLLSIRITGEADTGVCKEIFTQYICSFLWKVITWIKDGCLPLGKG
                     IDLTESENGVLRFMSTGMKGVWDSVGDSQQELADEYGNAQLNNLLGMGEQDVFRKVCL
                     AAFGYDWEINLDNVLDAAYHSPFATLVQPVLLSREYLTFDPVTYYSKYDYRGSWIINP
                     GCDLDNYEVYLACVTRSDLYRYDDIDCAKQSDPEGSNCDCLGLPPDKAPQPMFFYGSR
                     GRIKQNELINIDSTMIKNRIKSSAYRYDHLVFKLNVDRNMKKNNGDTSKCFTDGHEDG
                     TFYFPILDFSAQDIASCTVDATTGRFVCNLGPGFGNELGTASMNEVTVADSLTKNILE
                     PNGASYYAGDSRPISATVSYLKDEPMQCLIGRLYDRDRTTILDNRFVKLPKNQLSGQE
                     IITMKYMPSQQDVPERGYGFYMGYLDDKGDEQMAIGKLQYTPVERARKMGGGPLKFID
                     DPSNGPGVIVSRGSTDKFIYGNKEAKIGNHYVNGGLLVNISDMEASVSITTARDIPPT
                     SGSEYNYFIRYTGSSQEKASKEPRYWLHLDIRSPKTPDGNCQDVMGMEYDKVADGLDQ
                     TQVVISSGIRQEVDIPIYILPGMGEKEKCSSSYQLAPIQDNVQCACEDSSVKNCPDKN
                     GPGPQDDFNYCYNECRKYNRCMLNAPLDAPCVCSPDTRPDKYDCGVKDKNDANAYGIN
                     SMGKYCYEENSVLACRSAASQAGPEDTTPLTITWNSPQDNYMAKVNEVITIYATISDD
                     KVSGKEEAEIWVKSPDQENIQVIPFKDQNHREYWAITKDIQVKKPGEMIISIYAKDPD
                     SADPRPITIPPIRVKVSVI"
     gene            complement(8245..10209)
                     /locus_tag="VFF28_01490"
     CDS             complement(8245..10209)
                     /locus_tag="VFF28_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44335.1"
                     /translation="MTKKRGQITVFIIIGLVILLTYFLLMNYRKESIKEIDMIQPEFI
                     PIQDYVTKCTENLAREAIDIIGINGGYIYFPPWVQTNPNSYLKQSPIDSLKNPYWWFD
                     GTEAVPTLEFMTSQIEQYTKEGISGCVGEFSNFKDQYDIIPGKESFEVITDIGEEDIT
                     VKTIYPIEIRDKFNKTLAKITSFPVKIPIRLKKVHSLAKEILYRENRDYFIEKKAIDL
                     ISLDDDEVPTTGIAVQCGKKQWETGKVEKKIKQLLEKNLDYIKLKGSKYYSTSKITPY
                     QLKDTNPYSKSDTFNESYYNYHYIWDVTDQKLDNMHISFFYDQKWPIKFAVRPSSGKV
                     MRSNPQMAGKMLSAFCLHIWHFTYDVVFPVKVSIYDEKTERSEPYTFTFAFNAQINHN
                     EPDRSTFASEDYDTTSTIQEEEYCADVRNRVTIYAVDKVSRDEMREANLTFTCGRFIC
                     SMGMTDNDWKNDAQGTPQFTASFPYCMNGIIRANKPGYEEGMAFIQTGRLAGTEPEDN
                     IGGSFTVELRPVKEMKYTVVKHKIMDQAISGPKDLDSDEKAVIMLKNDKEAFNSESTY
                     FANTTIPIKILDGAAYDYDLEIYVIDNESISAGYKNKWSVTQNDLVLGKNITFHILYQ
                     DFKDEEDRLGFLASIAENSKKLPPPEIKTR"
     gene            complement(10206..10850)
                     /locus_tag="VFF28_01495"
     CDS             complement(10206..10850)
                     /locus_tag="VFF28_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44336.1"
                     /translation="MKRQQFLRFFICNLLLALSIMLLVKFTIIFTKPQFDKADAIDLE
                     NIALQTEEKMQETVEALKGFALFLLLAVILFVLAFIIIWSFFQSLAYHPFQLRFFEKF
                     LVLNVILIIPTTILFILSLYAGRNLIFPVILLFLALHFMLFIYPVFAKEPELRRIGAG
                     IKLSITKFPKLLIPYIAIAVTFIITSFINLDIISPLLYIVLFSWVQHYTRNLVR"
     gene            complement(10847..11329)
                     /locus_tag="VFF28_01500"
     CDS             complement(10847..11329)
                     /locus_tag="VFF28_01500"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HZX44337.1"
                     /translation="MDSKASLLLLRAGNEIRTGIVLINLSEKDNEKISLGATKEDTFY
                     SAVISHSYYAIFYAARAILLTKGIEPKAPEIHKKTLEAFKVNLVETGILDFELLKIYN
                     KMVLRADDLLALFQLEKQKRGDFTYKMISSANVEPAQESIENASVFIRNIREVMEQQR
                     "
     gene            complement(11313..11921)
                     /locus_tag="VFF28_01505"
     CDS             complement(11313..11921)
                     /locus_tag="VFF28_01505"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZX44338.1"
                     /translation="MMTKKEMKILEIFKGNLFSKYTIREMMKALNTRSYNWTYTAIIK
                     LKKENILKLERKGKSEICSINLDEQKTIAYLAFLEAVRPKNIPHEKKIIGFMPSDFHI
                     LIVAGSYANNTQTKESDLDLVAIIDKREERMQVLNKLQREGELMLPPLHPFVFTNKEF
                     LEMLAEKEENYGKEIFRKHIVITGAEFYFKLVKEAIDHGFKG"
     gene            complement(12053..>14257)
                     /locus_tag="VFF28_01510"
     CDS             complement(12053..>14257)
                     /locus_tag="VFF28_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44339.1"
                     /translation="EAQVNRAINKIKNEYSRASEEERRAMMEEVRMMRDSSSATAEAQ
                     VGQEALYGSLLDMNEGLDIAQRFGATYNHEDGSFTKNGVSGWAFTKDGDIDLTVQRPA
                     TTQQKEQVQKRNQDSMIELHNSIHKDDLLTRGQDGLIYDSAGNLITELRYNHVLKDTE
                     IKRAPPIKIIHSGKEQELTLVKDYTDDKGNPIYQTKDGTYFAQGSIWNDAQKLKPLEG
                     SIYTIEHIGTKEKPDQVLIRVGSELNGGKKTTTGLSYSIIQDPKQAKDMAKDIPLDKD
                     TLSLVRTSVAAGGSVKYEGDSKASSIVFKDSKGNGLKIVSFTGLEDDKDYVIAKDKMF
                     EGSKSTQEYRLSYDKNHDGTITDEEKEEEPIRVSFAYSSEDSQNGKRTKVYAADNFKF
                     GKDGKFTAEIVTQTFVEDKKSKTNVLSKDYSFYYIEVEESQADFKIEKKNGEPVAYDK
                     DDQVIAQSVIKVVGNDAHYVKADGSLESIDVDSEKAKRAITAYSKKESRRWFADFQFH
                     LTQFKGLSGWSQLIIPDEALNEWRENVDKVFSTAYLGTEYWTSAICKKHIPKENKGTF
                     LTQTKDSLFEVVAHVEGEKTVLPQPDGKIKYLYKFTYSVRNPKNSQYKSLRFNVYLDK
                     AIPIYRQSLDVKDGQVFSRGAGHYDKGTITYTKTEGKPVVQRSDNDYDSICIIFETGI
                     IDASGKPKGEICNQIVEYTGSPEEYEKRITQQTIAQPTPAGPSGDPYQEMEI"
BASE COUNT         3544 a         3079 c         2899 g         4735 t
ORIGIN      
        1 tgtgtgaatt tctatgtgtg ccataaaatt atcacctttg aaataattaa cggctacata
       61 tatttaaact tatcggtgat agaatctata tgtagcaggc aatatattaa gaagtattat
      121 taagggtttg gtctacttaa ccaataacct attctcttta tggattgtaa ggctaattta
      181 gttttcttta tagtgcaaaa tgtctgccat atcttagcgt ttgtttggta taggaaccat
      241 aacatttata aagaaattaa ctactacttg gtagtatatg gtatattata gctttcccta
      301 tagtcataat atcacatggc cgcccaattt tactgtcaag tatgggccaa cccctgtcga
      361 gacatggaac ccggggataa tccagcaaga tttctttgag caacagcagg ctatacatac
      421 cttgcctaga gaatattggc cagggccgtg tgaaagaatc ggctcctttg gggcaacgga
      481 aacatatttc aagtttgcct tatggaagat ggcttgcgag acagaccatt cactagagga
      541 aatagtacct cagaaaaccc tattatatgt ccaaaaatcc ggtgctgatc ctgtctttca
      601 gaatataggc gggcttacga tggctgatca ttacgggcat tttaatttta gagacgttgg
      661 aaagcttgaa acaaggattt ttgacataaa aaagaatgca gaagacttca tgaaaagccc
      721 tgaaggaagg agatttgcac ttgacaattc ggatctatta aaagatgccc cagaaatcga
      781 tgagatatgt gttaaagtaa attatcctgg aagggcttca atgctgcaac tttcagaaga
      841 tagagcaagg cttacaggga actataatat taaaaaatat gaagaagatg tggcaaacgc
      901 gctaggaatt ggcgtcgatt accagatgcg gagcactttt gcagaagaat ttcatcatct
      961 catcaggcga gatgtaataa aaagcacaag tcacggtatt aggatcgaag caagggtaag
     1021 ggagttattg atcgacatgt atggaaagat ggaggaagct actaaagatg ctggtgggaa
     1081 aaaagcctat ggcaggataa aagcgcatct tgaatgggag cttaagaaca tcaggaaact
     1141 ttatgagaaa gcatattcag aagaggatgg tgaattggaa tcaacgctgg acgctaaaaa
     1201 gcaatataac aaaggagaaa cacctcaagg ccttgaagag gtactggacg atgttagtgc
     1261 agaaacttgt gcagctgaag ctactatctc aaacggcgac aatggaagaa caagtggagc
     1321 tgaaagtgat ggcccaagtg gtgatggcgg aggagaaggc ggcggtgaat agctcactta
     1381 gccaactcat ttacccactc tatcaagaaa aggcgaagga aagctatcag cgccatcaat
     1441 actattatca ataaaataag aatcaacaag gaagcttcta tcgagatgaa aatcgcaaga
     1501 caaagcaagg ctatggctgt caaacatgca atgtaaatca tggttatttt gtgaatcttc
     1561 ttaaagccta aaacaatacc ttccttgagg aaattctttt ttgtcgaaat aaggcataac
     1621 atgccaaaaa acagatagcc cataattgcc agaaggatga ctgggcctgt taaggcaatc
     1681 atgttttctt ctgccaaagc ggacaatagc ttttcaaata gaacgccata cgcaagcaag
     1741 aaaaatatag ccagcgaaaa ggcacacttg gcaaagtagg aaagaaatct ctttttgtca
     1801 aataggactc tgtccgagag agaccatatc agcgaagtta gaagaatata acctgggata
     1861 gctatcagag ccaaaagctt gaagtattct accacccttc gatagctttg aaaaactatt
     1921 gcgggatcgt ttccaagata atcaaacatg ccagggtttt ctgtcaaatt tatcttgtcg
     1981 tagtaatcta cagcagcctt tgccttatcc atggcaggga ttatcgtaaa aaaggaagca
     2041 agggtgagca gtactattat ccccagctgc aacaggatga taaggcaaaa aagagattta
     2101 ttctttttta tcttatccag gacacttctt aatgaactta tcatatcacg cttaccttta
     2161 ctcttattgg agggatggta attggccttg gatctgcaga gtcagggtct tttgcatata
     2221 tggatattat catctcaccg ggctttttta cctggatatc cttggtgata gcccaatatt
     2281 cacgatgatt ttggtccttg aatggtataa cctggatatt ttcctgatca ggcgatttaa
     2341 cccatatctc agcttcctct tttccggaaa ccttgtcatc acttatcgtt gcatatatcg
     2401 tgatcacttc attaacttta gccatataat tgtcttgtgg tgagttccat gtgatcgtaa
     2461 gaggggttgt atcctcagga cctgcctgcg atgcagcgct cctgcaggca aggacagaat
     2521 tttcttcata acaatatttg cccatggaat ttataccata tgcgtttgca tcgttcttgt
     2581 cctttacccc gcaatcatac ttatcgggcc ttgtatctgg ggagcataca cagggagcat
     2641 caagaggtgc atttagcatg cacctattgt atttcctgca ctcattatag caataattaa
     2701 aatcatcttg cgggcctggg ccgttcttat cagggcaatt ctttactgat gaatcttcac
     2761 aggcacattg gacattgtcc tggattggag ctaactgata gctgcttgag catttctctt
     2821 tttcacccat gcctggcaag atatagatag ggatatccac ttcttgcctt atgccgctac
     2881 tgatgacaac ctgtgtctgg tcaagaccat ctgctacctt gtcatattcc atgcccatga
     2941 catcctggca attgccatca ggtgtttttg ggcttcttat atcaaggtga agccaatacc
     3001 ttggctcttt tgaggctttt tcctgagatg agcctgtata tctgatgaaa tagttatatt
     3061 cagagccgct ggttggaggg atatctcttg cagtagttat tgagacagac gcctccatgt
     3121 cgcttatgtt taccagcaat cctccattta catagtgatt ccctattttt gcttctttat
     3181 ttccgtagat gaacttgtca gtgctgcctc gcgacactat aactccagga ccattgcttg
     3241 ggtcatcgat aaatttcaac gggccaccgc ccatcttcct tgcccgttct acaggagtgt
     3301 actgcagttt acctatcgcc atctgctcat cgcctttatc atcaagatag cccatataga
     3361 atccatagcc tctctcagga acatcctgct ggcttggcat atacttcatg gttattatct
     3421 cttggccgct caactgattc tttggaagct tgacaaacct gttatccaaa atagttgtcc
     3481 tatctctatc atagagcctt ccaataaggc attgcattgg ctcatctttt agatagctta
     3541 ctgttgcact tataggcctt gaatctccag cataatagga ggcgccgttt ggctcaagga
     3601 tgttttttgt gagtgaatct gcgacagtga cctcattcat gctggctgtc cctagttcat
     3661 tgccaaaccc tggccctagg ttgcagacaa atcttcctgt tgttgcatca actgtgcagc
     3721 ttgcgatatc ttgtgcacta aagtctaata tagggaaata aaatgtccca tcctcatggc
     3781 cgtccgtgaa gcacttggat gtatcgccat tgttcttctt catgttcctg tcgacattga
     3841 gcttgaacac cagatggtca tatctataag cgcttgattt tatcctgttc tttatcatgg
     3901 tggagtcgat gtttatcaac tcattttgtt ttatccttcc tctgctgcca taaaagaaca
     3961 tgggctgtgg agccttgtct ggaggaagtc caaggcagtc acagttgctt ccttccgggt
     4021 cagattgctt tgcgcaatct atatcatcat atctgtatag atcgcttctt gtcacacagg
     4081 caagatagac ctcatagttg tccaggtcgc atccaggatt gattatccat gagcctctat
     4141 aatcatattt tgaataatag gtgactggat cgaatgtgag atattccctg cttaacagca
     4201 caggctggac aagggttgca aacgggctgt ggtaggctgc atcaaggaca ttgtccaggt
     4261 ttatctccca atcatagcca aatgcagcaa gacatacctt cctaaataca tcctgctctc
     4321 ccatgcccag caggttattt agctgggcat taccatactc atctgccagc tcttgctgag
     4381 aatctcccac actatcccat acgcccttca tacccgtgct catgaatctt aaaacaccat
     4441 tctcactttc tgtaaggtct attccttttc caagaggcag gcagccatcc tttatccatg
     4501 tgatgacctt ccataaaaat gaacagatat actgggtgaa tatctctttg catacgcctg
     4561 tatcagcctc gcctgtaatc cttatgctta acaggcaatt ttcgagggcg accatgaggt
     4621 ttcttatcat agaaagcctt gcaagtatct ggcttatcgc cacgcattgg aatgcagcta
     4681 tatgctgctt tgatgggtct atagtgagta tctttcctct gccatagaac cataggtcct
     4741 ggccaaagca tgcagaaagg tctcttcctt gcatatatct tagaggatta aaaaccttgt
     4801 ttaggtattt catcttgtaa tacctgtagc tccatctcat atcttcataa ctgcttgcct
     4861 ctgggccgcc gtctttactc tctacgtctg ctggctgata gtaaccgggg aaggctcctg
     4921 ctttcgaatt caggcagcag cattcaaccc tcctgtctgg attgtcgctt acttggtcag
     4981 cctgccagca atcgcttgtg tatcccatag gcatggcaga ttttatctca accggtgtgc
     5041 tttgaccttc aacctggaac tttgcatttt cgttgcttgc atatgagagg cattcatttg
     5101 tttttgcgca gccagacgcc attatcaatt tttcaccttg cttatcactt tgagtaactc
     5161 cttttggctc actgccaggg gtgattatct gagcatcctt ttttcctact ttaaacccag
     5221 cagttgacct tttgccagta atgtcctggc tgcaaatctc ctcgcaagtc tgctccatgt
     5281 gggtgaggta atttttttca ttctgcttga taacaaaatc aacgcctatc ttctcatcgc
     5341 caccaacaac agatacgctg taaaggtcat ttcctgcatc tatcggagag tctatcttgt
     5401 aaagagtcat atatttcatt cctttctctg ttgaagacac ctcaaggcat ttatcgtccc
     5461 tatcaaaatc cctgggatta gggtattttt ttgctgttgc ctgacaatcg actgccctta
     5521 gaggccttcc tcttacagac tggtctgtgg cacagcttga gccagaggct gctttctcaa
     5581 acaaagcgat gtcatcctca ctttctgtcc actttgatgg tgttggatgg ccaaagacac
     5641 ggtcgcatgc ccatctgtaa gtctggtata ggctctcttc tgctccccat actgaggcgc
     5701 agcttgggta gcataccttg aggcaggcat cgctcaagtc atcctggaaa tccttctttc
     5761 ctttagcacc ctctttactt ccctcttcat atttcttcga tgctgatttg ctgtcttgta
     5821 tgcatttatt gcactcttca tttgtgcctg atgtgccgcc aagcttttgc acatccttat
     5881 atacactatc ataggcacag accatccttc tgtagatctg caatacaaat tttgtaaaga
     5941 atgtaaccat gcatgcaagc gctgcccact caaggactct tcttatcttt aggatccaat
     6001 cattgagtac gcctatagac ttgttgaggc tgtcagtgaa atcataaagg agccagtcag
     6061 gcaggatctc tcttgggttt atcttaactg catcgagctg gtatgagact tctgtgcata
     6121 tctggtaatg gccatactgc tttgtcttat tgttgatatc ttcgtcataa taatccaacc
     6181 ttagcttgaa tgggaatgcc atctcttctt ttacagcctg gataaaactt tcccaatcac
     6241 tatcatgcca ggattcaaca ccctccaatc ttccaaggcc gcatgccaca taagcggtct
     6301 ttccgttagg gcttagtttt tcagtgcagc tccttatcag tttgcagcta tgattaaacc
     6361 tgtccaaact ctccagatat cccttgccgc atgctttttc tatgttaagg tttgtaactt
     6421 ttgcatcctt gccctggccg tgataggtga aattgagata aaaatagata gcctctgtgc
     6481 cttcttttag cctctcgaca ctcaggaagg taggtgattg atactcaacc aatggtgttg
     6541 catcccatgt aagcaggggt gaccagcagg tagtgatggt gtagtctgcc tgcaagaatc
     6601 ctttcctttt tgcaggatcg actgcaacga agaaaagcct gctttgcaat gaatcatgag
     6661 tttccttgaa ttttacaaag tcataataag gctctcctgt aggcagctct tcaacactta
     6721 ctgctcccgc ccatatcgaa gtaaggtcga cattttctat tacgaaattt ccgttttcgt
     6781 cagcgactgc ttcatagtca gcatgggtct tgcattgctc ttttccgtct atctctgccc
     6841 ggcagtttgc cctcaactgt gcttcgtcta actgagagat atagtcaggg gcgccataag
     6901 catcccatgt tgtgttgaaa tatgaaaaag gcgttctgta catatataag taaatccttg
     6961 cgcctggctc tgacttgcct gaaatatcga ccatatcggc gtagttttga aagatatttg
     7021 catttgcctt tggagagatt atcttaattt ctggatcttt tgtatcaaga aggattgtct
     7081 tttcaagcag ccattggttt tttgccctgt ctatggcatt tatgttaatg gtgtagtaac
     7141 catcttcttt acccaggtca aactcaaggc tgaagtcttt ttgaacctct attgtctttt
     7201 tactgcctat ctctattttt acattctctt gaggcaggtc ttttctgcct ccttcagggg
     7261 tccttatcat tatggaatcg gacttttctc ctatcaggcc aaacttgttc atggctgcga
     7321 ctctataaga gtatgtattg cctgaatttg caagaacatc tgaataatca ttatagccgg
     7381 aatcagagcc gatgcccagg ggagtatcgt tcctgtaaac tatgtactgc tcaaagtcct
     7441 ttatgtcaac cttgtcccag gaaaggtcta ccttatttgg ctttactgat gtattcctca
     7501 ggctttgcac ctttggaggc atctctggct ttgttgtgct actccttact gagtaagata
     7561 tgtttacagg ctcgtcggct gtgccgttga tggaaaactt ctcctttccg acaatgtcag
     7621 ggatagatga aatattaagt atagggtctt ttgtgtcgag gataaccttg taaatctttt
     7681 ctgctgactg gccctgtgac tttgctgtca gctttattgt gttttcaccc tcgatgagag
     7741 cgatagaggg aaacccgaat gagccgtcat cgcctgccaa aagcttcctt ggttcatctg
     7801 cattaacaaa aaggtttacc tcagcaagcc ttattgtctt tcctgatact gatattgtct
     7861 tcttgcttag gtatgatgga agggttgcat cgataaaaac cggctggttt ggaagggttg
     7921 tgaactgata ataattgccg ttgttgttgt cagtgacttc ttcagacttt acttcaaaaa
     7981 aataaggggc attcacatca agccctgtca gggcgatgtc atggacatcg tcaaaggcat
     8041 tgctttgcct gtttttgcca agctcttttg tcgctccgta attgacacta ccttttgcat
     8101 ttgtgtttgt ttcccacttt acccttgcag agttttgtgt cacttgttct actctcacat
     8161 tagttatgac cagggattct gctaaggtga taggcagagt gataactaga aagatcaaga
     8221 atatgcaaac catctgtctt gttttcatcg ggtctttatc tccggcggag ggagtttttt
     8281 tgagttttca gcaatacttg ctaaaaaccc aagcctatct tcttcatcct taaagtcctg
     8341 atagaggatg tggaaggtga tgttttttcc aaggacaagg tcgttttggg tcactgacca
     8401 cttgttttta tagcctgctg agatgctctc attgtctatt acatatatct ctagatcgta
     8461 atcatatgct gcgccgtcta gaatcttgat gggtattgtt gtgtttgcaa agtatgtgct
     8521 ttcactgttg aatgcttctt tgtcattttt tagcataatc actgcttttt cgtcgctgtc
     8581 cagatctttt ggcccgctta tggcctgatc cataatctta tgcttaacaa cagtgtattt
     8641 catctctttt acaggcctta gctcgactgt gaagctgcct ccgatgttat cttctggctc
     8701 tgttcctgca agccttcctg tctggataaa tgccatgcct tcttcatagc cgggcttgtt
     8761 tgcccttata atgccgttca tgcagtaggg gaatgatgct gtaaattgag gggtgccttg
     8821 agcatcgttt ttccaatcat tgtctgtcat gcccatgctg cagatgaacc ttccacaggt
     8881 aaatgtcagg tttgcttctc gcatctcatc tcttgacacc ttgtctactg cgtaaattgt
     8941 gactctgttc cttacatcag cgcagtactc ctcttcctgg atggtgcttg ttgtatcata
     9001 gtcttctgaa gcaaatgtgc ttctgtcagg ctcgttatgg tttatctgcg cgttgaatgc
     9061 aaaggtaaat gtatagggct cacttctctc tgtcttctca tcgtagatgg agaccttgac
     9121 agggaaaact acatcatagg taaagtgcca tatgtggagg cagaatgcag aaagcatctt
     9181 tcctgccatc tgagggttgc ttcgcataac ttttccgctt gaaggcctta cggcaaattt
     9241 tatcggccat ttctggtcat agaaaaagct gatatgcata ttgtcgagct tttggtctgt
     9301 cacgtcccat atatagtgat aattataata ggattcattg aaggtgtcgc ttttcgagta
     9361 agggtttgta tctttgagct ggtaaggggt tatttttgag gtgctgtaat acttactgcc
     9421 ttttagcttg atataatcca ggtttttttc caggagttgc ttaatctttt tttccacctt
     9481 ccctgtttcc cattgttttt ttccgcattg gacagctatt cctgttgtgg gcacttcatc
     9541 atcatcaagg ctgatcagat cgatcgcttt tttttcaata aagtaatccc tgttttccct
     9601 gtataatatc tcttttgcta ggctgtgcac cttttttagc ctgattggaa tttttaccgg
     9661 gaaactggtt atctttgcca gggtcttgtt gaacttatcc cttatctcta tagggtagat
     9721 ggtttttact gttatgtcct cttcacctat gtctgttatc acttcaaagc tctctttacc
     9781 cgggattatg tcgtattggt ccttgaaatt tgagaactca ccgacgcatc ctgatattcc
     9841 ttcttttgtg tactgctcta tctggctcgt cataaactca agggttggaa ctgcctctgt
     9901 accatcaaac caccagtaag gattttttaa actgtctatc gggctttgct ttagatagct
     9961 gtttgggttt gtctgcaccc aaggtggaaa gtagatataa ccgccgttga tgcctataat
    10021 atctatcgct tcccttgcca ggttttctgt gcattttgtc acataatcct ggatagggat
    10081 aaactctggt tggatcatat ctatctcttt tatcgattcc ttcctgtaat tcataagaag
    10141 gaagtaggtg agcaagatga ctagccctat tattatgaac actgtaatct ggcctctttt
    10201 tttagtcatc ttactaagtt cctggtataa tgctgaaccc agctgaatag cactatgtag
    10261 agcagcggtg aaatgatatc taaattaatg aatgatgtga ttataaaggt tacggctatt
    10321 gctatgtaag ggatgagcag ctttgggaac tttgttatag agagttttat tcctgcgcct
    10381 atccttctta attctggctc tttggcaaac actgggtaga tgaagagcat gaagtggagt
    10441 gcaagaaata gtaaaatgac tggaaagatc aggtttcttc ctgcgtagag agatagtatg
    10501 aaaaggattg ttgtggggat gatgagtatt acatttaata ccaggaattt ctcaaaaaac
    10561 cttaattgaa aaggatgata tgccaggctt tggaaaaaag accagataat aataaatgca
    10621 aggacgaata atataactgc aagcagcaaa aagagggcaa agcctttcaa tgcttctaca
    10681 gtctcctgca tcttctcttc tgtctgaagg gctatatttt ccagatctat tgcgtctgct
    10741 ttgtcaaact gaggttttgt aaaaattatg gtgaatttta ccagcagcat gatagagagg
    10801 gctaataata ggttgcagat gaagaacctt aggaattgct gccttttcat cgctgctgct
    10861 ccatgacttc tctaatattt cttataaaaa ctgaggcatt ttctattgat tcttgggcag
    10921 gctcaacatt tgctgaagag atcatcttat aggtaaaatc tcctcttttt tgcttttcca
    10981 gttgaaaaag ggccaagagg tcatctgccc tcagtaccat cttgttataa attttaagca
    11041 gctcaaaatc aagaatgcct gtttcaacaa ggtttacctt aaatgcttca agggtttttt
    11101 tgtggatttc cggcgctttt ggctctatac cttttgtgag caggattgct cttgctgcgt
    11161 agaatatcgc atagtatgaa tgagagataa ctgcagaata aaatgtgtct tcttttgtcg
    11221 ctccaaggct tattttttcg ttatctttct cagagaggtt tatgaggaca attcctgtcc
    11281 ttatctcatt tcctgccctc agtaaaagca gactagcctt tgaatccatg gtcgattgcc
    11341 tcctttacca gcttaaaata aaattccgct ccggttatta ctatatgctt cctgaatatc
    11401 tccttgccat aattctcttc cttttctgcc agcatttcta aaaattcctt gtttgtaaac
    11461 acaaaggggt gaagcggcgg aagcattagc tctccttctc tctgcagctt atttagcacc
    11521 tgcatccttt cctccctttt gtctatgata gcaactagat ctaaatctga ttcctttgtc
    11581 tgtgtattat tagcataact gcctgcaact attagtatgt gaaagtcaga tggcataaag
    11641 cctataatct tcttttcatg ggggatattt tttggcctta ctgcttccaa aaaggcaaga
    11701 taagctatcg tcttctgttc gtctaggtta attgagcata tctcgctttt acccttgcgt
    11761 tccaacttga ggatattctc tttttttagc ttgattattg cagtatatgt ccagttatat
    11821 gagcgtgtat taagagcttt catcatctct ctaatcgtgt acttgctgaa taaattgcct
    11881 ttaaagattt caaggatctt catctctttt tttgtcatca ttttgctatc ctgattttag
    11941 gataatatta tccttttttt ggtatacatc aaaaactccc tgagtttaag catgctcaga
    12001 aatcgctggc ttacttttgg ttgaagcacc atagaatgcg attgctgaca tttcatattt
    12061 ccatctcctg atatggatct ccactgggtc ctgcgggtgt cggctgagct attgtttgct
    12121 gggtaatcct cttttcgtat tcttcagggc tgcctgtgta ttctactatc tggttgcata
    12181 tttcgccttt tggcttgccg cttgcatcta ttatgcctgt ctcaaagatt atacagatgc
    12241 tgtcgtaatc attatcactc ctttgcacaa ctggctttcc ttctgtcttt gtataagtga
    12301 tagttccttt gtcgtaatgt cctgcgcctc ttgaaaacac ctggccgtct tttacatcaa
    12361 ggctttggcg gtatataggt atggccttgt cgagatagac attgaatctt aaagacttat
    12421 actggctgtt ctttgggttt cttactgagt aggtgaattt gtagagatac tttattttgc
    12481 cgtctggctg aggcaacact gtcttttctc cctcaacatg ggcgacgact tcaaacaggc
    12541 tgtcttttgt ctgcgtcagg aaggttccct tgttctcttt tgggatatgc ttcttgcaga
    12601 tagcgcttgt ccagtactca gtgcccaggt atgctgttga aaacaccttg tctacatttt
    12661 ctctccactc gtttagggct tcgtcaggga taatcaattg agaccagcct gagaggcctt
    12721 tgaactgggt gaggtggaac tggaagtcgg cgaaccatcg gcgtgattct tttttagagt
    12781 aggcggtgat ggctcttttt gccttctcac tatctacatc aattgactcg agtgatccgt
    12841 cagctttcac atagtgtgca tcgttgccaa caacttttat gaccgactgg gcaattactt
    12901 ggtcatcttt atcataggca actggctctc cgtttttctt ctctatcttg aaatccgctt
    12961 gagactcttc aacttcaata tagtaaaagc tgtagtcctt ggaaaggaca tttgttttgc
    13021 tttttttatc ttcaacaaat gtttgtgtga caatctctgc tgtgaatttc ccatccttgc
    13081 caaacttgaa gttgtcagca gcatagactt ttgtcctctt accattttgc gaatcttctg
    13141 aagagtaagc aaatgatact ctaataggtt cttcttcctt ttcttcatcg gtgattgtac
    13201 catcatggtt cttatcatag ctcagcctat attcttgagt agattttgag ccttcaaaca
    13261 ttttatcttt agctatcaca taatctttat catcttcaag acctgtaaag gatacaatct
    13321 ttaggccatt gccctttgag tctttaaaga cgatgcttga agctttgctg tctccttcat
    13381 actttacaga accacctgct gctacggaag tccttacaag ggagagggtg tctttgtcga
    13441 gggggatgtc cttggccata tctttagctt gtttgggatc ttggattatt gagtaagata
    13501 gtcctgtagt ggttttcttg cctccattaa gttcagaacc tacccttatc aaaacctgat
    13561 caggtttttc ctttgtgccg atatgctcta ttgtgtagat ggagccttca aggggtttta
    13621 atttttgtgc atcattccat attgaacctt gagcaaaata cgtcccatct tttgtctggt
    13681 agattggatt tcctttatca tcagtataat ctttcactaa agtcagctcc tgctctttac
    13741 ctgagtggat aatcttgatt gggggcgctc gtttgatttc tgtgtctttt aagacatgat
    13801 tgtacctcaa ttcggtaatc aagtttcctg cactatcata aatcagccca tcttgccctc
    13861 ttgttaagag atcatctttg tgaatagagt tatgaagttc aatcatacta tcctgatttc
    13921 ttttttgcac ttgctctttt tgctgagtag ttgcgggtct ttggacagtg aggtcaatgt
    13981 ctccatcttt agtgaacgcc caaccactta ctccattttt tgtgaaactt ccatcttcat
    14041 ggttatatgt ggcgccaaac ctttgtgcaa tatccaaacc ctcattcata tccaacaggc
    14101 ttccgtaaag ggcttcctgg ccaacttggg cttctgcggt tgccgaagaa gagtctcgca
    14161 tcattctaac ctcttccatc atggcccttc tttcttcttc gcttgccctt gaatattcgt
    14221 ttttaatctt atttatcgcc ctatttactt gagcttc
//
LOCUS       DASRUK010000033         3463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_30022, whole genome shotgun sequence.
ACCESSION   DASRUK010000033 DASRUK010000000
VERSION     DASRUK010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30022"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VFF28_01515"
     CDS             complement(<1..265)
                     /locus_tag="VFF28_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZX44340.1"
                     /translation="MKKTLHRISYAKNRREATKAYWDFADKWRRNYPNAVKCLETDIE
                     DLLTFFHIKEDRLWSKIRTTNAIERTFREVRRRTRPIGVFTNME"
     gene            complement(464..913)
                     /locus_tag="VFF28_01520"
     CDS             complement(464..913)
                     /locus_tag="VFF28_01520"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZX44341.1"
                     /translation="MPRTRNKGFYPSVLDAYKRRHRSIDEVILACFVLGLSTRKAAAV
                     LAPMIGEAISASTVSRIARSLDKHVPACIKQGEVERYHNRPLSDRYRFLFFDGVVLKN
                     KGAAKVQRRPLLCAYGITHEGICEIIAPDRFNRGHLRENSRRHGKDF"
     gene            complement(924..1166)
                     /locus_tag="VFF28_01525"
     CDS             complement(924..1166)
                     /locus_tag="VFF28_01525"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZX44342.1"
                     /translation="MLHRATVRNVSRALKEVNCFQWEGEFKLKAKEALKKVLEDRMGE
                     ELVEYIGVESYQRDDTRSDYRNGSYTRHLLCEIGDL"
     gene            1381..2139
                     /locus_tag="VFF28_01530"
     CDS             1381..2139
                     /locus_tag="VFF28_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44343.1"
                     /translation="MPSAKNNMKLDKSFEEIIKERFVHSLSNIIYDKVSALWHVDKES
                     EKFMARIAANVVLGTFCLEYLSHINLLIKEKAKITENITAADLSDKDLMTKVKAALSG
                     NKEAASLFRNAASQLKKIIEVADIKTLEDLTKIAHTSDYLLSSLQDMKDSNHDDYAIW
                     LSSIPYYYEKDLMHLGLMKEARFKAQDIGLDLDSYYSTPVWNAEFPAEPFYTEKIYSN
                     HRINLNAHRATKEGYFPCIQSKGKGKRINSRARS"
     gene            2142..2567
                     /locus_tag="VFF28_01535"
     CDS             2142..2567
                     /locus_tag="VFF28_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44344.1"
                     /translation="MRIKEKVQGIDFLLKKICANRNPLPTPKLKTRNVEMKREIKHSF
                     DIPLQPNKRFSILVKDKVSFGPGKGPFAIEIVVVGFVFLKEEMNASQKKALLKDSENA
                     NFLINQCLPHTSAAVAYLTDRMGFSPIILAPKFPISREG"
     gene            2623..3306
                     /locus_tag="VFF28_01540"
     CDS             2623..3306
                     /locus_tag="VFF28_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44345.1"
                     /translation="MEDDPDIKAIISILLNKRKQDFYNYASIICFCYKEEDLINLPDV
                     KHLPGKVKAWIKRKSFDQDAAYITFFKHLFSLYSGFVQTYEGWDKLGKHRSVIPEAYV
                     YHRIKERYASRSDVKVERECFVIIDGWDSRIDRTKDEGQKVDVGVWDYTAQHGEVYEV
                     KVKVYIKEKEHQLEFLETIRKVSNKSIDVFLASFAPKHVVLQHLKTFFPGRDLSQINI
                     LGVDELQNL"
BASE COUNT         1015 a          718 c          689 g         1041 t
ORIGIN      
        1 tctccatatt ggtaaacacc cctataggcc gtgtccttcg tcttacctct ctgaatgtcc
       61 gttcaatggc attggtcgtc cttatctttg accacaacct gtcttctttg atatgaaaaa
      121 aggtcagcaa atcttctata tcagtctcca aacatttgac cgcattcgga tagttcctac
      181 gccatttgtc tgcaaaatcc caatatgcct ttgttgcttc tctcctgttc tttgcatagg
      241 aaatcctgtg gagggtcttt ttcactaact cccaatcctt ctttcttacc ttatccagta
      301 tgttccttgt tccccatttt catgaggaca ggcttatgcg cccagcatct ttgtcttaat
      361 atcttgggat atacatactc caatgcgcta tgaagccctt taccgccgtc ggtaattatc
      421 agttcgcagc tctttccgaa tatccctctt ttgtagaggt cgtttaaaaa tccttcccat
      481 gcctcctgga attctccctg agatgccccc gattgaatct atcgggggct attatctcac
      541 atatcccttc atgggttatg ccgtaggcgc ataaaagagg cctcctctgc acttttgcag
      601 cccctttatt ctttaatacc accccatcaa agaatagaaa ccggtatctg tccgatagcg
      661 gcctgttatg gtatctttct acctctcctt gcttaataca tgcagggaca tgtttatcca
      721 gacttctggc aattctgctt acggttgatg cgcttatggc ttcccctatc ataggggcta
      781 ataccgctgc cgcctttctg gtagacagcc ccaatacaaa gcaggcaagg attacctcgt
      841 ctatgctccg gtgtcttctc ttgtaggcat ccaagacaga cggatagaat cctttgtttc
      901 ttgtccgagg cacacttcta atatcacaaa tcccctatct cgcagagaag atgccttgtg
      961 tagctgccat tgcggtaatc tgagcgagta tcgtctcttt ggtaagactc aactcctatg
     1021 tattcaacaa gttcttctcc cattcggtct tccagtacct ttttgagcgc ctccttcgcc
     1081 ttgagtttga attccccttc ccactgaaaa cagttgactt cttttaatgc acgacttaca
     1141 tttcttaccg ttgctctgtg caacataggg tgtattcctc ctttcccgtt taaaaacata
     1201 acgggacaag tttttatctt gctgataatc taggagaata caccctcttt tattattcat
     1261 caatgaattt acacagagaa gaatacatta cctaggttcc ctttaatctt gacaaatatt
     1321 ttgccttgtc ttatatttgt tctatcctta acaagtagta agtattgtta atagctagcc
     1381 atgccatctg caaagaataa catgaaatta gataaatcat ttgaagaaat tataaaggaa
     1441 aggtttgttc acagtctgtc caatatcatc tatgacaagg tttcagctct ttggcatgtt
     1501 gataaagaat ctgaaaaatt catggcacgt atagctgcga atgtagtgct agggactttt
     1561 tgcttggagt atctttcaca catcaatttg ttgataaaag agaaggccaa gataacggaa
     1621 aatattacag cagcagattt atcagataag gatttaatga ctaaagtaaa ggctgctctt
     1681 tctggcaata aagaagcggc aagccttttc agaaacgctg ccagccagtt aaaaaaaatc
     1741 atagaagtag cagatataaa aactcttgaa gatttaacaa aaatagccca tacaagcgat
     1801 tatcttttat cgtccttgca ggatatgaag gattccaatc atgatgacta tgcaatatgg
     1861 ttatcttcaa ttccctatta ttatgaaaaa gatttaatgc atctcggcct tatgaaagaa
     1921 gcacggttta aggctcaaga tataggtttg gatttagata gctactattc gacacctgtt
     1981 tggaatgctg aatttcctgc tgagccattt tatactgaaa agatatattc caaccaccgc
     2041 attaacctta atgcacaccg agcaacaaag gaaggttatt tcccctgcat ccaatcgaaa
     2101 ggcaaaggca agaggatcaa ttccagagca aggagctaga tttgcgtatt aaagagaaag
     2161 tccagggcat tgatttcctc ttaaagaaga tatgtgctaa tagaaatccc cttccaacgc
     2221 cgaagcttaa aacaagaaat gtagagatga agcgagagat aaagcactcc tttgatattc
     2281 cgcttcagcc aaataaaaga ttttctatac ttgtcaaaga caaggtttcg ttcgggcctg
     2341 gcaaaggacc gtttgctata gagatagtgg tagttggttt tgtgtttctt aaagaagaaa
     2401 tgaacgcatc tcagaaaaag gccttattga aagatagtga aaatgcaaat ttcctcataa
     2461 atcaatgcct gcctcacaca agcgctgcgg tcgcctatct taccgacagg atggggttta
     2521 gccctattat ccttgcccca aaatttccca tatcaaggga gggataaagc cttgcatgtc
     2581 gactttagcg tagacgaaag gtctttgcga tacatagaat ccatggaaga tgatccggac
     2641 ataaaggcga ttatttccat tctcttgaat aagcggaagc aagattttta taactatgca
     2701 tcaataatat gcttttgtta caaggaagaa gaccttataa atctgcctga tgtcaaacat
     2761 cttcctggca aggtgaaggc gtggattaag cgaaagagtt ttgatcagga tgctgcttat
     2821 attaccttct ttaaacacct attttcgctt tactcgggtt ttgtccagac atacgaaggg
     2881 tgggataagc ttggtaaaca caggagcgta attccagaag cttatgttta tcacaggata
     2941 aaggagaggt atgcctcacg gtcggatgtt aaagttgaaa gagaatgttt tgttatcata
     3001 gatggatggg atagccgtat tgacaggaca aaagacgagg gacaaaaggt tgatgtcggc
     3061 gtgtgggatt atacagcaca gcacggtgaa gtctatgaag taaaggtcaa ggtgtatatt
     3121 aaggaaaagg agcatcagct tgaatttctc gagacaataa ggaaagtctc aaacaagagc
     3181 attgatgtct ttctcgcaag ttttgcccca aagcatgttg tgcttcaaca cctcaagaca
     3241 tttttccctg gaagggattt aagccagata aatatattgg gggtagatga gttgcaaaac
     3301 ctgtaagttg taattacgaa ttaggaatta cgaatgacaa atggtgattt tttaatacct
     3361 aattcctaat tgttttagag gggtttcatg aaaaaagagg taataagcac agatatggcg
     3421 cctaaggcaa tagggccata ttcacaggct gtaaaggtaa atg
//
LOCUS       DASRUK010000034        16083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_30838, whole genome shotgun sequence.
ACCESSION   DASRUK010000034 DASRUK010000000
VERSION     DASRUK010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 16083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16083
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30838"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..463
                     /locus_tag="VFF28_01545"
     CDS             122..463
                     /locus_tag="VFF28_01545"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZX44346.1"
                     /translation="MTHINENMVKFFSALSDPTRLNILLSMVEGPRTVNEIYSHIGRQ
                     KMTLSAISHQLKDMQDIGLIEFEKKGREKFFQLADRFCWCILRDALKHFHDSKNCPAC
                     QKSKKLMQVRK"
     gene            460..1272
                     /locus_tag="VFF28_01550"
     CDS             460..1272
                     /locus_tag="VFF28_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44347.1"
                     /translation="MSLKIPIAITLIVISLGAFVYFTGEREMETEGAKKEGAMEEAEK
                     KAEKKMEGKESSTDEILSDISIKVAYTEEGTIKIFALAKNNDLADLKAYAGKSIPEKD
                     SIVIGYSEAKLMTDENLFSKPGDPINGLSGIDTKVEGILAKTNTEIDDFYFVSATQFR
                     SIAGDENAVLVSKEEGMNRIIYSYQGNSNVKFNLNQGDLKDYGVHEIAGVKYYPALLG
                     SKEAKQFEKDKIKVGDFVKIMGKNAVIVGVIEETGTALDMMILFPFVIGDEQ"
     gene            1269..3455
                     /locus_tag="VFF28_01555"
     CDS             1269..3455
                     /locus_tag="VFF28_01555"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZX44348.1"
                     /translation="MKKTDLLISGMHCASCATLLTKALSKKEGVKEANVNYGTAKAAI
                     TFDEKKTNEEELIKIVEKKGYKASVSDGKIDVEKQQKAQQEEIKGIKRMFYFSLVFAI
                     PIFLITMILMWTGVMLPYKDFILFALSTPVQFIAGWQFYTGTWSALKNKSANMDTLIA
                     VGTSAAYFYSLFVLFFQPGEEQYFEISAILITLVVLGKLLESVAKGKTSEAIKKLMGM
                     TPKTAIVIRGKKEIEIAVDDVVVGDIVLVKPGERIPVDGEIVDGYSAVDESMITGESI
                     PVEKKKGDEVFGATINKHGAFKFKTTKVGANTTLASIIKLIEDAQGHKAPIQRFADMI
                     SAYFVPIVIVLSIITFGVWYLLLAAELSFAVIAAVAVVVIACPCALGLATPTAIMVGT
                     GKGAQHGILIKGGEALETAHKLKYIIFDKTGTITNGTPEVTDIVTLDGSKEGKVLQLA
                     ASIEKNSEHPLAEAIVKKADKIHLSAAKKFKAIPGHGVTAQIDRTTYYFGNPKLMKDQ
                     SINIAKYTDQIHSLEEDGKTVMVLSDSKKAIGLIGVADQIKDTSAEAVKMLNKLGIKV
                     YMITGDNERTAKAIARKAEIENVFAEVLPEDKAKYVKKLQANGKVAMVGDGINDAPAL
                     AQADIGIAMGSGTDVAMETGNIVLMRNDLLDVPRAIKLSKFTMGKIKQNMFWALIYNV
                     LGIPVAAGIFYAWTGWLLSPIIAGGAMALSSVSVVSNSLLLKRKKL"
     gene            3456..3992
                     /locus_tag="VFF28_01560"
     CDS             3456..3992
                     /locus_tag="VFF28_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44349.1"
                     /translation="MQRYIKWAIALLGTLIATPPRALAHCPLCTIAAGAGLGISRGLG
                     VDDGVIGLFFGAFIASSALWLSSWLGKKVEMPMLEMITLILSFLLSVVPLYLAGIITN
                     IYEVMKYPQTSLLGLGYLGIDRLLAGIILGTIGIAGAFKLSDWIKKKRGKVMWSYQGL
                     SFMAAALIILTSILLVIT"
     gene            3917..4465
                     /locus_tag="VFF28_01565"
     CDS             3917..4465
                     /locus_tag="VFF28_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44350.1"
                     /translation="MELPGLVIHGSCTYYPNLNTIGDNMKNFITLIEDIKKKGELDIS
                     AGEDLSIALMNLISLEEHFSFSYMKTEEEKYLNFLEKVREVRKELMNKIVSKEEKDYS
                     EKWCISKHLLASSMRLMETGTKCLHDDKKEEAKDMMVKAFDLYSIFWALNGSNMLKAP
                     ASKSRLSEISNMMKKLIDCCKE"
     gene            4462..4809
                     /locus_tag="VFF28_01570"
     CDS             4462..4809
                     /locus_tag="VFF28_01570"
                     /inference="COORDINATES: protein motif:HMM:NF025170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCYCGC motif-containing (lipo)protein"
                     /protein_id="HZX44351.1"
                     /translation="MKQTRYGRTLSIAVLLILLVPTALLFTMKKPVSTGTPWFIERNP
                     TIKEAYEFAAGNPDALNGVPCRCGCFSIPHDGRLHKRGLLDCFFREDGSYDIHASQCS
                     SCIEDALKVKGQQ"
     gene            4863..6002
                     /locus_tag="VFF28_01575"
     CDS             4863..6002
                     /locus_tag="VFF28_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44352.1"
                     /translation="MENSFEHKVISEDRIGKADMSEIASGGARSDALAQSKPAISEAQ
                     EHAPNFRSAVLDNSEPDQHISEDYEKKDSDAEITLPPMPAAGSARLWQIISAILAILL
                     IFAFFRSPGSNLTGAATADSVKAATLTFINENLLQDGSTATVKSMAQGNGLYILKLDI
                     SGREIESYVSTDGKLLFPSAIDMTRPLQNAQAEQPSQPVPKTDKPKVDLYVMSFCPYG
                     NEAENTMLPVYKLLKDKVDFNVKYIVSASGDTIQSLHGQTEVEQNARELAVAALYGKD
                     KLWDFMAAINTNCGRDASCWETEAKNLGMDAQKIKDYTAKQGTDALKKEAQESGANGV
                     QGSPTLIINGQETQAVYNYGNPEAYKAAICSAFNSAPAECKQAVN"
     gene            6117..7241
                     /locus_tag="VFF28_01580"
     CDS             6117..7241
                     /locus_tag="VFF28_01580"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZX44353.1"
                     /translation="MRSILIILISFILAGCANEYSQNQPIETKDNLVEQHWNNVFTKA
                     FLEVDCPPVRDPNSLPDGYYKGPMIDTHIHLQSLPDGEPGQPDYYGYNLGIKRSIDEW
                     ICMMDVEGTKQAWGFFPVWEPIIEESIDVVNRTMQAYPGRFVPFIMPPDDDGSLEGYP
                     TVDSIELERMLNIKKGMFKGFGEIGLYGREDGSSALPPDSQRLQKIYPIIRKHKLTIY
                     FHLGEGQDKALESVAKANPDISFVFHGDQLIDCAACSKKPDVVAGMLERNPNIYYGVD
                     ELYGNVWLLKPRSSKESFISHFKDYEPLLNKDLENWKEFIEKHPDQVIWGTDRGVSEI
                     WDIDPDVALTLNNYTRGFIGRLNLSVQEKFAYKNAEKLIS"
     gene            7599..9947
                     /locus_tag="VFF28_01585"
     CDS             7599..9947
                     /locus_tag="VFF28_01585"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HZX44354.1"
                     /translation="MKIKTTLWIICILLFSMASYADSGAQGLIGTLKLINAEYADAVK
                     DEKVIDEGEYLETEIFLEKALDLYEKQEKSSEMTERLEVIKSTVAQKGDPSIVNENIN
                     KIIAILQEQSGSGTVSEDAKGEANQQSLADSELSGEQVIGGLRVGLIIDEAEDFYLPV
                     DGELVKNPAEAQHLKVVLREKDTKRMIPYTLVSASIQGNNGWKQEIILHPLWGRFFYY
                     GNNANLADGRYKAIIIIEHGGLAHHELDKWIEPIKAVFEFNVKDGVARIDNQPGIATT
                     EGFLPGDDIDIAVAELWEERKAGKYSIGFIAESVEEIYAFKSGKLVSTVKGSELYHLE
                     AVVREASTGRILPYADISMQLTKGKESLEYNLVPMWAEFFHYAANADVDEGIWHVKVM
                     VKPSILGYLDKEDAFEEATVEFTFDTSRISGGGIAEIKGLIGEAINEYKEGNTQEASK
                     LAKEAFITFEEGVGKEISARDSSLEDEIEKDVLALSRIKEGYSDIDKLASNIYSNLER
                     AQGILESKSNAKFLYLQSLTIIVREGFEAIIIIAAIIAYLAVSGNKQKISIIYKAAGL
                     AILASLLTAWLIEKVFGLGFASREILEGATMLIAVIVLFYVSYWLVSKIQADRWQRFI
                     EGKVADAITKGNKFLLGSVAFLAVYREGFETVLFYNALLIDAPASPIIAGFLSGIIVL
                     CIVYFILKRYSTKIPVKQFFIITSAILYYMAFTFMGKGIAELQEGGVVPMSPLSWAPD
                     IPVLGMYPTVETFVMQSILVIALAASLLYTFLWKPLKADGLK"
     gene            10233..11723
                     /gene="hcp"
                     /locus_tag="VFF28_01590"
                     /gene_synonym="priS"
     CDS             10233..11723
                     /gene="hcp"
                     /locus_tag="VFF28_01590"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HZX44355.1"
                     /translation="MFCNQCEEVIDNIGCTREGACGKKEEISVLQDKLVRMARVISKK
                     GSNTEEANVFIMESLFSTITNVNFDAEHFKERLAKGREYVSISDDELIENRSDDKDTL
                     ALKDLLTHGLKGLCAYLDHAYILGYKDDTIFGFIREALDTEGKTAEELTALVLKCGEY
                     GVRAMALLDKANTETYGHPEPTKVSLDVGANPGILISGHDLKDMQRLLDQTDGTGVDV
                     YTHGEMLPAHAYPAFKRYKHLRGNYGGSWWKQNEEFESFNGPILMTTNCLIPPKESYK
                     DRLYCTGLVGFEGVKHIGKDFSSLVVHAKKCKAPIKIEEGEITVGFGHSAVLSVADKI
                     VEAVKNGAIKRFVVMGGCDGRKNRDYYTEFARALPSDAVILTVGCAKYRYNKLPLGDI
                     GGIPRVIDAGQCNDSYSLVVIAKKLADVFGLDDINKLPISYNLSWYEQKAVLVLLALL
                     SLGVKNIHLGPKLPAFISPNVLNVLVEKFGVSGISNVEHDVNKLAL"
     gene            11786..12382
                     /locus_tag="VFF28_01595"
     CDS             11786..12382
                     /locus_tag="VFF28_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44356.1"
                     /translation="MNQRLGFILLVSFLVFGCSKPVEQMTAEERLAKIEYIGDKTDFM
                     VPPDYVSLEKNIDILLGMGGRIGPKHYDELEAAVDRLERQGQDVSVLRNKLTQLDVAP
                     NQKPVPYNQSTEILSERNIDNNNDSNKGQRFNESNNAQQNNQSNQAPFKLGLLSPRGC
                     EGEGPRMLGASPIALSDLQKILPMGMMSTQHITPTDHQ"
     gene            12578..12955
                     /locus_tag="VFF28_01600"
     CDS             12578..12955
                     /locus_tag="VFF28_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44357.1"
                     /translation="MKAIAVDGKIPEQTWERIPVKEGEVIGSVGVGKFDFSVVDEAVN
                     LTGFANPKTYEEVWKIHTVDTFDYYKEPLRSALLAKNVRVDKPYGGKIDYDIKGRLIG
                     NWFKEGTGGGFMRDRFVGQYENI"
     gene            12981..13319
                     /locus_tag="VFF28_01605"
     CDS             12981..13319
                     /locus_tag="VFF28_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44358.1"
                     /translation="MAPNISKITTAAEYRSLKQGDYVAEMFGAMIQIGRVQSVRNTPQ
                     MYDSTAGRRIPAGAYAVIQLASDKELTRIAGVDIVFAIPEHLKDKLDLLADMISRASQ
                     DLKETAQRVA"
     gene            13404..14273
                     /gene="hisG"
                     /locus_tag="VFF28_01610"
     CDS             13404..14273
                     /gene="hisG"
                     /locus_tag="VFF28_01610"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HZX44359.1"
                     /translation="MKLKLGIPKGSLQESTVALFKKAGFNISVNSRSYFPSIDDEEVE
                     CMLIRAQEMARYVEDGVLDCGLTGIDWVMENNADVVKVSDLIYSKRGLGKVRWVLAAP
                     VGGKIKSVKDLEGGKIATEAVNIAKKYLKKNKVNADVEFSWGATEVKPPELADAIIEI
                     TETGSSLRANNLAILDTVMESNTVLVSNKKSWKDEKKKRKMEEIALLLQGAIAAEEKV
                     GLMMNVKKVDLQNVLAALPALKNPTISELADKGWVAINTIVDEKTVREIIPKLKKAGA
                     QGIVEYPLNKVIL"
     gene            14270..15517
                     /gene="hisD"
                     /locus_tag="VFF28_01615"
     CDS             14270..15517
                     /gene="hisD"
                     /locus_tag="VFF28_01615"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HZX44360.1"
                     /translation="MKIISASNHKEIEKVMKRGNDLDSAVEKVKPVLEDVRKDRDKAL
                     FKYIEKFDGKRPAILLVAKKELKEAFERVDTAVIRAIKQSKENVERYAKLQMPKGWTK
                     EVSKGVVVGQLVRPLDSVGCYVPGGNFPLISTVVMTVVPAKVAGVKEVVVCTPKDNDD
                     IYAACYLCGVDKVYRIGGAQAIAAMAYGTESVQKVEKIVGPGNIYVTAAKKLVNGDVG
                     IDFLAGPSEVMIIAEKGNAKFIAADMLAQAEHDMMASAVLVTTDKKLAQEVNEQIDIQ
                     LKELDSEIAKKSIENYGMIIIVKNIEEAISLANEFAPEHLEIIVKDKERVIPELQNFG
                     ALFIGEYSPEAAGDYCSGPNHVLPTGGVARYRSGLSVLDFLKMPTVQELSREGLVSLR
                     KSITKLAALEGLRGHKVSVEIRD"
     gene            complement(15514..15918)
                     /locus_tag="VFF28_01620"
     CDS             complement(15514..15918)
                     /locus_tag="VFF28_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44361.1"
                     /translation="MNIPDRSIKRIAAVLILLSAVGYIAERHALLPWQGQERAYRNIG
                     ELEAEVRLFYQHPSPEKMNRVKARIYSAMRSAAYLGATPDEQREILEKALYYSFMVRS
                     VIGANAELRPGQESIDTCIMEINNTIEQTISH"
BASE COUNT         5121 a         2981 c         3870 g         4111 t
ORIGIN      
        1 aaagggtata tccagcgaag cttatatgac tccatgatgc attttaagga cgggtaatat
       61 aaaaatattc tgattacact tccataaggt ttataaagtt gagcaaatgc tcaactgttc
      121 aatgacacat ataaatgaaa atatggtgaa gtttttttca gcactgtcgg atccaacacg
      181 gcttaacata ctgttgagca tggtagaagg gcctaggaca gtaaatgaga tatactccca
      241 tataggcagg cagaagatga ccctttctgc aatatcccac cagcttaagg atatgcagga
      301 catcgggctg atagaatttg agaaaaaagg aagggaaaaa tttttccagc tggctgatag
      361 attctgctgg tgcatactaa gagatgcact taagcacttc catgattcaa agaactgccc
      421 tgcctgccag aagagcaaaa aacttatgca ggtgagaaaa tgagcttgaa gataccaata
      481 gcaataacat tgatagtaat ttcgcttgga gctttcgtat attttaccgg cgaaagagag
      541 atggagacag agggcgccaa aaaagaaggt gccatggaag aagctgaaaa gaaggctgaa
      601 aaaaagatgg aaggcaagga atcgtccaca gacgagatcc tgtcagacat ctccataaaa
      661 gtagcatata ccgaggaggg aactataaag atatttgcgc ttgccaaaaa taacgatctt
      721 gccgacctta aagcttatgc aggaaaatcg atacctgaga aggattcaat agtcatcggc
      781 tattcagaag ccaagctgat gaccgacgaa aatctgtttt caaaaccagg cgatcccatc
      841 aacgggcttt ctggcataga tacaaaggtt gaaggaatac tggcaaagac taatacagag
      901 atagatgatt tttactttgt cagcgcaacc caatttcgca gcattgcggg tgatgagaac
      961 gctgtcttag taagcaaaga agaaggaatg aacaggataa tctactccta tcaaggcaac
     1021 tccaatgtaa agttcaacct caaccaaggg gatttgaaag attatggggt gcatgagata
     1081 gctggagtca aatattatcc tgcccttcta ggaagcaaag aagctaagca gtttgaaaag
     1141 gataagataa aagttggaga ttttgtaaag ataatgggaa agaatgcagt catcgtggga
     1201 gttatcgaag agacaggcac agcccttgac atgatgattc tgtttccatt tgtaataggt
     1261 gatgaacaat gaaaaaaaca gatctattga taagcggcat gcactgcgca agctgtgcaa
     1321 cattgctcac aaaagcgcta tccaaaaaag agggcgtaaa ggaagcaaat gttaattatg
     1381 gcactgcaaa ggcggccatc acgtttgatg aaaaaaagac taatgaagaa gaactgatca
     1441 agatagtcga gaaaaaggga tacaaagcat ctgtcagcga tggaaagata gatgttgaga
     1501 aacagcagaa agcgcagcag gaagagatca aaggcataaa gcgtatgttt tatttcagcc
     1561 ttgtgtttgc gatccccata ttcctcatca caatgatact tatgtggaca ggtgttatgc
     1621 tgccgtataa ggatttcatc ctttttgcgc tgtcgacccc ggtacagttc atagcgggat
     1681 ggcagtttta tacagggaca tggtctgcac tgaagaacaa gagcgcaaat atggacacac
     1741 tgatcgctgt cggaacaagc gctgcatatt tttacagcct gtttgtgttg tttttccaac
     1801 caggagaaga gcagtatttt gagatctcag cgatcctgat aaccttagtc gttcttggca
     1861 agctcttgga atctgtcgct aaaggaaaaa cttctgaagc tatcaaaaag cttatgggga
     1921 tgaccccaaa aaccgccatc gtcataaggg gcaagaaaga aattgagata gctgtcgacg
     1981 atgttgttgt gggcgatatc gtcttggtca agcctggcga aagaatccct gtcgatggag
     2041 agatagtgga cggctattca gcggtcgatg aaagcatgat aacaggcgaa agcatccctg
     2101 tagagaagaa aaagggagat gaagtgtttg gagcgaccat aaacaagcat ggcgctttca
     2161 agttcaagac aacaaaagtg ggagcaaata caactcttgc aagcatcata aagcttattg
     2221 aagatgcaca ggggcataag gcgccgatac agcgctttgc ggatatgata tctgcatatt
     2281 tcgtgccgat agtgatagtc ctttcaataa tcacctttgg agtatggtat ctgctgcttg
     2341 ctgcagagct gtcttttgcg gtcatcgcag ctgttgctgt tgttgtcatc gcatgcccat
     2401 gcgcacttgg gcttgccacg cctacagcga taatggtagg gacaggaaaa ggcgctcagc
     2461 atggcatcct tatcaagggt ggagaggcgc ttgagactgc gcataagctt aagtacatca
     2521 tctttgacaa gactggaacg ataaccaatg gaacacctga agtcactgac atcgttactc
     2581 ttgatggaag caaagaagga aaagtgctgc agcttgctgc atcgatcgag aagaactccg
     2641 agcatccact ggcagaagca atagtgaaaa aagcagacaa gatacaccta tctgctgcaa
     2701 agaagttcaa agctatccct ggacatggag tcactgccca gatcgatagg actacttatt
     2761 attttggaaa tcccaagctt atgaaagacc agagtataaa catagcaaaa tatacggacc
     2821 agatccattc attggaagag gacggaaaga cagtgatggt cctttcagac agcaaaaaag
     2881 caatagggct tattggagtt gctgaccaga taaaagatac gtctgcagaa gcagtgaaga
     2941 tgcttaacaa gcttgggata aaggtctaca tgataacagg agataacgag agaaccgcaa
     3001 aagcaatcgc tcgaaaggca gagatagaga atgtctttgc agaagtgctt cctgaagaca
     3061 aggcaaaata cgtaaagaag cttcaagcaa acggcaaggt agcgatggtc ggagacggca
     3121 taaacgatgc ccctgctctt gctcaggcgg atatcggcat cgccatggga tcaggcacag
     3181 atgttgcgat ggagacagga aacatagtgc ttatgagaaa cgacctcttg gatgttccaa
     3241 gggcgataaa gctaagcaaa ttcaccatgg gtaagataaa gcagaacatg ttttgggccc
     3301 ttatctacaa tgtgcttgga atacctgttg ctgccggaat attttatgcc tggacaggat
     3361 ggctgctttc gccgataatc gcaggcggag cgatggcact tagctcagtc agcgtcgtca
     3421 gcaattcgct attgctaaag aggaagaaac tttagatgca gagatatatc aaatgggcta
     3481 tcgcactgtt aggtaccttg attgccacac cccccagggc tttagcccat tgccctcttt
     3541 gcactatagc tgcaggagca gggcttggaa tctcaagggg gcttggagtg gacgacggtg
     3601 tgatcggcct tttttttgga gcatttatcg cttcatctgc attgtggctg agcagttggc
     3661 tgggaaaaaa ggtggagatg cctatgctgg agatgataac ccttatctta tcttttcttt
     3721 tatctgtggt tccgctatac cttgcaggaa ttatcactaa catatacgag gtgatgaagt
     3781 atcctcaaac atcgctttta ggactcggat atctgggcat cgatagattg ctcgcgggga
     3841 taatcctggg tacgatagga attgcaggtg cgttcaagct gagcgattgg ataaaaaaga
     3901 aaagagggaa ggtcatgtgg agctaccagg gcttgtcatt catggcagct gcacttatta
     3961 tcctaacctc aatactattg gtgataacat gaagaacttt attaccctga tagaggacat
     4021 caagaaaaaa ggagagttgg atatatctgc gggcgaagac ttaagcatag ccctcatgaa
     4081 ccttatctct cttgaagagc acttttcctt ctcatatatg aagacagagg aagaaaagta
     4141 tcttaatttt cttgagaaag tgagggaggt aagaaaggaa ctgatgaata aaatagtgag
     4201 caaagaagag aaggattaca gtgaaaagtg gtgcataagc aagcatctgc ttgcaagctc
     4261 tatgagactt atggagacag ggacaaaatg tttgcatgac gacaaaaagg aagaggctaa
     4321 agatatgatg gtaaaagcgt ttgaccttta tagcatattc tgggcattga acggctcaaa
     4381 tatgctaaag gcgccagcct cgaaatcaag gttatcagaa atatcaaaca tgatgaaaaa
     4441 attgatagat tgctgcaagg aatgaaacaa acgagatatg gacggacatt aagcatcgct
     4501 gtgctgctga tacttttagt tccgactgct ttgctgttta ccatgaaaaa acctgtaagc
     4561 acaggcactc cctggtttat tgagaggaat ccaacgatta aagaagctta tgaatttgcg
     4621 gcaggaaacc ctgatgcctt gaacggagtc ccgtgcagat gtggatgctt cagcataccg
     4681 cacgatggca ggttgcacaa aagggggctc cttgactgct tttttaggga ggatggatct
     4741 tatgatatcc acgcttcaca gtgcagttca tgtatagagg acgcgcttaa agttaaaggc
     4801 cagcaataaa agaggcaaga ttaacaatat ttaaaagagg ataaagatta gaggtgattc
     4861 ccatggaaaa tagctttgaa cacaaggtaa tatctgagga tagaatcgga aaggctgaca
     4921 tgtcagaaat cgcttctggg ggcgcacgct ctgatgcttt agcccagagt aagccagcga
     4981 tttctgaggc tcaagaacat gccccgaact ttaggtcggc ggttcttgac aattcggagc
     5041 cagatcaaca cataagtgag gattatgaga aaaaggattc agatgcagag atcaccctcc
     5101 cacccatgcc ggcagcaggt tcggcaaggc tgtggcagat aatatctgca atcctagcca
     5161 tacttcttat attcgccttt tttaggtcgc caggaagcaa tctcactggt gctgcgacag
     5221 cagattcagt taaggcagct acactcactt ttatcaatga aaacctgctc caagatggat
     5281 ctacagcaac tgtaaagtca atggctcaag gaaatggact atacatacta aaacttgata
     5341 tcagcggaag ggaaattgaa tcttatgtga gcactgatgg aaaactcctt tttccttcag
     5401 caatagatat gacaagaccc ctccaaaatg cacaagccga gcaaccatct caacctgtgc
     5461 caaaaactga caagccgaaa gttgacttat atgtaatgag cttttgcccg tatggaaatg
     5521 aggctgaaaa tacgatgctt ccggtgtata aacttctaaa agacaaggtt gactttaatg
     5581 tgaagtatat tgtcagtgca agcggcgata ctatccaatc attacatggg caaactgagg
     5641 tggagcagaa cgcaagagag cttgctgtag ctgcacttta tggaaaggat aagctatggg
     5701 atttcatggc tgctatcaac actaactgtg gaagggacgc aagctgctgg gaaactgaag
     5761 caaagaacct tggaatggac gctcaaaaga taaaggatta tactgcaaag caagggaccg
     5821 atgctttgaa aaaggaagcc caggagtctg gtgcaaatgg cgtccaaggc tcgccaaccc
     5881 ttatcatcaa cggtcaggag acccaagctg tgtataatta tggcaatcca gaggcataca
     5941 aggctgcaat ctgcagtgca ttcaacagtg cgccggctga atgtaaacaa gctgtaaact
     6001 aatgcctaga agatgttata tatttggagg gggatgatta ttttatctcc ttcttgttgt
     6061 tttttctact catgaccgca gaaaggttaa tatatcaagc ggcctaaaaa ctaaggatga
     6121 ggagcatact cataatcctt atctctttta tccttgcagg ttgcgctaat gaatattctc
     6181 aaaaccaacc tatcgaaacg aaggataatc tagttgagca gcactggaac aatgtcttta
     6241 ccaaagcttt cttagaagtc gattgccctc ctgtgcgaga tccaaacagt cttcctgacg
     6301 gctactataa aggccctatg atagatacac atatacattt gcaaagcctg ccggatggag
     6361 aaccaggcca gcctgattat tatggataca acctggggat aaaacgaagc atcgatgagt
     6421 ggatatgcat gatggatgtt gaaggaacaa agcaggcttg gggatttttc cctgtatggg
     6481 agccaataat agaggaatct atcgatgtag tgaacaggac aatgcaggcg tatccaggga
     6541 ggtttgttcc atttatcatg ccgccggatg atgatggcag cctggaaggc tatcctactg
     6601 ttgattcaat agagcttgaa aggatgctaa atatcaagaa gggtatgttc aaaggttttg
     6661 gggaaattgg gctttacgga cgagaggatg gttcttctgc tcttccccct gattcgcaaa
     6721 ggctgcaaaa gatatatcct attatcagga aacataaact aaccatctac tttcatctag
     6781 gcgaaggcca ggataaggcg ctagaatcgg tggccaaagc aaatcctgac atcagctttg
     6841 tctttcatgg tgaccaactg atagactgcg ctgcttgcag caaaaaacct gatgttgttg
     6901 caggcatgct tgaaagaaat ccgaatattt attatggtgt cgatgagctt tatggcaatg
     6961 tctggctgct aaaacctaga agcagcaagg agagctttat ttctcacttt aaagattacg
     7021 agccgcttct taataaagat cttgagaatt ggaaggagtt tatcgagaag catccagacc
     7081 aggtgatatg gggaacagac aggggtgtat ctgaaatctg ggatattgat cctgatgtcg
     7141 cgctgacctt aaacaattat acacgagggt ttattggcag gcttaacctt tcagtgcagg
     7201 agaagtttgc atacaagaac gctgaaaagc tgatttcttg aattgctcaa gacttgggtt
     7261 cataccccgt agcttgctac ggcttaataa cccagtttga ggcttaaaaa ataccccgca
     7321 gcttgctgcg gttgggattt tttattgctc aaactaaagt acatactccg cggcttggct
     7381 agcgatatct cagtttgagg cttaatgctt gccgttttgg gtttttggaa aaagaagccc
     7441 ttgaagcctt gatagattat caaaaggcgc tcatgatggc cagtttaatc cactgatgcc
     7501 ttccgaagta tgaaccgtag aaaatagcaa attctagata tccacggcat aagttaacaa
     7561 tagtaaactt tttatataag ttaggcgaac ctaactttat gaaaataaaa acaacacttt
     7621 ggataatctg catcttgttg ttttctatgg caagctatgc agattcaggc gcacaaggtc
     7681 ttatcggtac attaaaactt atcaatgcag aatatgcaga tgcagtcaag gatgaaaaag
     7741 taatagatga aggagaatat cttgagacag agatattcct tgagaaggct cttgatctgt
     7801 atgaaaaaca agaaaagagc agcgagatga ccgagcggct tgaagtaatc aaaagcacag
     7861 ttgctcaaaa gggagatcct tctattgtta atgagaatat taacaagata attgccatct
     7921 tgcaggagca gtcaggttca gggacagtat cagaggatgc aaaaggtgag gcaaaccagc
     7981 aaagccttgc agactcagag ctttcaggtg aacaggtaat cggagggcta agagtgggcc
     8041 ttataataga tgaggctgaa gacttctact tgcctgtaga tggtgaactt gtcaaaaatc
     8101 ctgcagaagc ccaacatctg aaagttgtct taagagagaa agatacaaag aggatgatac
     8161 cttatacttt agtaagcgca tctatccaag gaaacaatgg atggaagcag gagataatac
     8221 tgcatccttt atggggaagg tttttttatt atggaaacaa tgcaaatctt gctgatggaa
     8281 gatacaaggc aattatcatt atagaacatg gcggccttgc tcaccatgag cttgacaaat
     8341 ggatagagcc tatcaaggca gtctttgagt ttaacgtaaa agatggggtt gccaggatag
     8401 ataatcagcc gggtatagcg acaaccgaag gctttttgcc aggagatgat attgacattg
     8461 cggttgccga actttgggaa gaaagaaagg ccggtaaata ttctatcggt tttattgctg
     8521 aaagtgttga ggagatatat gcatttaagt caggaaagct ggtcagcact gtaaaaggct
     8581 ctgaacttta tcatcttgaa gcggttgtgc gggaggcatc tacaggaaga atcctcccct
     8641 atgcagatat tagtatgcag cttacgaagg gaaaagaaag ccttgaatac aatttagtgc
     8701 ctatgtgggc tgagttcttc cattatgctg ccaatgcaga tgttgatgaa ggaatttggc
     8761 atgtaaaagt tatggtcaaa ccctcgatcc ttggctatct tgacaaggaa gatgcatttg
     8821 aagaagctac tgtagagttt acctttgata caagcaggat atctggcggg gggatagctg
     8881 agataaaagg gctgataggt gaagctataa atgaatacaa ggaaggcaat acccaagaag
     8941 cctcaaagct tgcaaaagag gcatttataa cttttgaaga aggcgtggga aaggagataa
     9001 gtgccagaga cagttcgctt gaggatgaga tagagaaaga tgtccttgcc ctttcacgca
     9061 taaaagaggg atactcagat atagacaagc tggcgtcaaa tatttactct aacctggaga
     9121 gggcgcaagg catcctagag tcaaaaagta atgcaaaatt tttatacttg caatccctta
     9181 caatcattgt aagagaggga tttgaagcga taataatcat cgcagcaatc atcgcttatc
     9241 tcgctgtttc aggcaataag cagaaaatta gtataatcta taaggcagca ggccttgcca
     9301 tacttgccag ccttcttacc gcatggctta tagagaaagt gtttggcctt ggatttgcaa
     9361 gcagggaaat actcgaggga gcaacaatgc ttatcgcagt tattgtgctc ttttatgtta
     9421 gctattggct tgtaagcaag atacaggcag acaggtggca gagattcatc gaaggcaagg
     9481 ttgcggacgc gattacaaaa ggaaacaagt ttttgcttgg atccgttgcg tttcttgctg
     9541 tataccggga gggatttgag acagtccttt tctacaatgc actgcttatt gatgcgcctg
     9601 caagcccaat aatcgctggc tttttaagcg gtataatcgt gctctgcata gtttatttca
     9661 ttttaaagag gtatagcaca aagattccgg tcaagcaatt ttttatcata accagtgcta
     9721 ttctatatta catggctttt acatttatgg gcaaaggaat agcagagctt caggaaggag
     9781 gtgtagtccc aatgagccct ctctcatggg caccagacat ccctgtgctt ggaatgtacc
     9841 ctactgttga aacgtttgtg atgcagtcga tacttgtcat tgcgctggca gcatctttgc
     9901 tgtatacatt tttgtggaag ccccttaagg ctgatggttt gaagtagtag gatacaggct
     9961 tgttcaagct ttatgctctg gtcagaagaa cacctgatca tcataagtct gtcaggcata
    10021 aatatacttg acgaaagttt agcgcatgga agtatactgc ttttcaagtt atgaaataga
    10081 gcgatattat ttccttgcgt taatatcctg tgcgatcgat agattgccac ttgccaagcc
    10141 taaaagatgc tgagtggatg ccttaaggtt tcctccaaga aaccgcccat atataaataa
    10201 cctccactgc acattcatac gaggtggtta ttatgttttg caatcaatgc gaagaggtga
    10261 tcgataatat agggtgcaca agagagggtg cttgtggaaa gaaagaggag atttctgtgc
    10321 tgcaggataa gctagtgaga atggcaaggg ttatatcaaa aaagggatcc aacactgaag
    10381 aagcaaatgt ctttatcatg gaatcattgt tttctaccat caccaatgtc aactttgatg
    10441 cagagcattt taaagaaagg cttgctaagg gaagagaata cgtatctata tcagatgatg
    10501 aattgataga gaatcggtca gatgataaag ataccctggc attaaaggat ttgcttactc
    10561 atggcctgaa gggactctgc gcctatcttg accatgctta tatattaggc tataaagatg
    10621 atacaatttt tggatttatc cgagaggcac ttgacactga gggtaagact gctgaggaac
    10681 tgactgctct tgtcttaaaa tgcggtgaat atggtgtaag agcaatggca ctgcttgaca
    10741 aggcaaatac tgaaacctat ggacatcctg aaccaacaaa agtaagcctt gatgtaggtg
    10801 ccaatcccgg tattttgata agcggccatg acctaaagga tatgcagagg cttcttgatc
    10861 agacggatgg aacaggcgta gatgtttata cacatggaga gatgcttcct gcgcatgcat
    10921 atcctgcttt caaaaggtac aagcatttgc gaggaaacta tggaggctca tggtggaagc
    10981 agaacgaaga gtttgaaagc tttaacggac ccatccttat gaccaccaat tgccttatcc
    11041 ctccaaagga aagctataag gataggctat attgcacagg gcttgtaggg tttgaaggtg
    11101 tcaagcatat cggaaaggat ttttcttcct tggttgtgca tgcaaaaaaa tgcaaggctc
    11161 ctatcaagat tgaagaagga gagataacgg tcggatttgg acactctgct gtgctgtctg
    11221 ttgcagataa aattgttgag gctgtcaaga atggtgccat caaacgcttt gtggtgatgg
    11281 ggggatgcga tgggcgaaag aatagggact attacacaga gtttgccagg gcgcttccaa
    11341 gcgatgcagt catattaaca gtagggtgtg caaagtatag gtataacaaa ctgccgctag
    11401 gagatatagg agggataccc agggtaatcg atgcaggaca atgcaatgac agctacagcc
    11461 ttgttgttat tgccaaaaag cttgcagatg tgtttggcct tgatgatata aacaagcttc
    11521 cgatttcata taacctatca tggtatgagc agaaagcagt gcttgtcctt cttgcgctgc
    11581 tgtctctagg tgtaaaaaac atacatcttg ggcctaaatt gcctgctttt atatcaccaa
    11641 atgtgcttaa tgtgcttgtt gagaagtttg gtgtatcagg tataagtaat gttgaacatg
    11701 atgtaaacaa gctagcgctt taacccatgc caaagtcgca aaaccttatg ataattatat
    11761 aaaccaggct atcatcaaaa gacgcatgaa tcaaagatta ggttttattt tgctcgtctc
    11821 ttttcttgtt tttggctgtt ctaagcccgt agagcaaatg acagctgaag aacgcctggc
    11881 caagatcgag tatatcggcg ataagacaga ctttatggtt cctcctgact atgtctccct
    11941 tgagaaaaat atcgacatcc ttctaggcat gggaggtagg attgggccaa agcactatga
    12001 cgagctggaa gctgctgtcg acaggcttga acgccaagga caagatgttt ctgtgcttag
    12061 gaataaattg acccaacttg atgtagcgcc caaccaaaaa cctgtgcctt ataatcaaag
    12121 cactgaaatt ttgagcgagc gtaatataga taataataat gatagcaaca aagggcagag
    12181 gttcaatgag tcaaacaatg ctcagcaaaa taatcaatcc aatcaagcac ccttcaagtt
    12241 gggattatta agccctcgcg gttgtgaagg cgaaggtcca cgaatgcttg gagcatcacc
    12301 tatagcactc tctgatcttc aaaagatact ccctatgggg atgatgtcta cacagcatat
    12361 cactccgaca gaccatcagt aatttaatac ccttgatttt gcgggcccaa acgaagacaa
    12421 gattaactta gataggttca agatctatgc gcctgctgat ggagcgatag ttgagatagg
    12481 aaagccattc aatgccaatg actataagat aatcatcgag cactcatgca atttctatac
    12541 tatatttata catatagaca aattgtctga caaagttgtg aaggcaatcg cggttgatgg
    12601 caagatccca gagcagacat gggaaagaat accagtcaag gagggagagg ttattggctc
    12661 tgtaggcgta ggcaagtttg acttctctgt tgtcgacgag gcagtcaatc ttactggttt
    12721 tgccaatcct aaaacatatg aagaggtgtg gaagatacac actgtagata catttgatta
    12781 ttacaaagag ccgctaagaa gtgctttgct tgcaaagaac gtcagggtcg acaagcctta
    12841 tggcggtaag atagattatg acatcaaagg aaggttgata ggcaactggt ttaaagaagg
    12901 cactggtgga ggatttatga gagatagatt tgtagggcaa tatgaaaata tttaaaagct
    12961 gactgatgtt tcccccattt atggcaccta atattagcaa aataacaaca gctgctgaat
    13021 acagatcttt aaaacaagga gactatgttg ctgagatgtt tggagctatg attcagatag
    13081 gccgggtcca atcagtaaga aatactcctc agatgtatga ttctacagct ggtcggcgta
    13141 tacctgcggg tgcttatgca gtaattcagt tagccagcga taaagaatta accaggattg
    13201 ctggggttga tattgtattc gcaattccag aacatctaaa ggataaactt gatctactag
    13261 cagatatgat aagtagagca tcgcaagact taaaagagac cgctcaaaga gttgcataac
    13321 aagatttcta ttttttaaaa cagtaatata gcggttaaat ttaaatatag aagtagctaa
    13381 ttttaccttg ttaaggtgat acgatgaaac ttaaactagg aatacccaag ggaagcttgc
    13441 aggaaagcac tgttgcattg tttaaaaaag caggatttaa catatctgtc aactcaagga
    13501 gctactttcc atctatagat gatgaagagg tagaatgcat gcttatacgt gcccaggaga
    13561 tggcgcgcta tgttgaagat ggcgttttgg actgcggcct aacaggcata gactgggtta
    13621 tggagaataa tgcagatgtt gtaaaagttt cagacctgat ctactcaaaa agaggccttg
    13681 gaaaagtcag atgggtcctt gcagctccag ttggaggcaa gataaagtca gttaaagatc
    13741 tagaaggtgg caagatagca acagaggcag taaacatcgc caaaaagtat cttaagaaaa
    13801 acaaagtcaa cgcagatgtt gaattctcat ggggagcaac agaagtaaag ccgccagaac
    13861 ttgcagatgc aataatagag ataacagaga ccggctcatc cttaagggca aataacctcg
    13921 ccatccttga cacagttatg gaatcaaata cagttcttgt ctcgaacaaa aagtcatgga
    13981 aagatgaaaa gaagaagaga aagatggaag agatagcttt gctgctgcaa ggggcgatcg
    14041 ccgcagaaga aaaagttggc cttatgatga atgtaaaaaa ggtggatttg cagaatgtcc
    14101 ttgctgctct cccagcactt aagaatccta ccatatcaga gctagctgac aaaggatggg
    14161 ttgccataaa cacaatagtt gatgaaaaga cagtaagaga gataatacct aagcttaaga
    14221 aagcaggagc acagggcata gtcgaatacc cccttaacaa ggtaattcta tgaagataat
    14281 ctcagcttca aatcataaag agatagaaaa ggtgatgaag cgaggcaatg atcttgactc
    14341 tgccgtcgag aaagtcaagc ctgttcttga agatgttagg aaggataggg ataaagcgct
    14401 cttcaagtac atagagaagt ttgatgggaa aagacccgca atcctgcttg tggcaaagaa
    14461 agagctaaaa gaagcatttg aaagagttga cacagccgtg ataagggcca taaagcaatc
    14521 caaggaaaac gtggaaagat atgcaaagct tcagatgccc aagggatgga caaaggaagt
    14581 ttctaaaggt gttgttgtag gtcagttggt aaggcccctt gattctgttg gctgttatgt
    14641 tcctggagga aactttccgt tgatatctac tgtagttatg actgttgttc ctgccaaggt
    14701 tgctggtgta aaagaagtgg tggtttgtac gcctaaagat aatgacgata tatatgctgc
    14761 ttgctatctc tgtggtgtcg acaaggtata caggattgga ggagcacagg ctatagcagc
    14821 catggcctat ggcacagagt cagtacaaaa agtggaaaaa atagtaggcc caggcaacat
    14881 ctatgtgaca gccgcaaaaa agcttgttaa tggtgatgtt ggaatcgact ttcttgctgg
    14941 accttcagaa gtgatgataa tcgcagaaaa aggaaacgct aagtttatcg ctgctgatat
    15001 gctagcccag gcagagcacg atatgatggc ctcagctgtg ttagtgacca ccgataaaaa
    15061 actagcacag gaagtgaatg aacagataga catacaactt aaagagttag attcagagat
    15121 agcaaaaaaa tccattgaaa actacggcat gataatcata gtcaaaaata tagaagaagc
    15181 gatttctctt gcaaacgagt ttgctccaga gcacctggag ataattgtca aagataaaga
    15241 gagagtgata cctgaacttc agaactttgg cgccttgttc ataggagaat attcaccaga
    15301 ggcagcagga gactattgtt caggcccaaa ccatgtcctt cctacaggtg gagttgcaag
    15361 atatcgctca gggttaagtg tgctcgactt cttgaagatg cctacagtcc aggaattgag
    15421 cagggaagga cttgtttctt tacgtaaaag tataactaag ctcgctgcac ttgaagggtt
    15481 gaggggacat aaggtaagtg tagaaataag agattaatgg gatatagttt gttcaattgt
    15541 attatttatc tccattatgc aggtatctat agattcctgc ccagggcgaa gttctgcgtt
    15601 ggctccgata acagacctaa ccataaagga ataatatagg gccttctcaa ggatctctct
    15661 ttgttcgtca ggagtcgccc ccaaataggc agcagaccgc attgcgctat atatcctggc
    15721 tttcactcga ttcatttttt caggactggg atgctggtag aataacctta cttctgcttc
    15781 aagttcaccg atattcctat atgcacgctc ttgaccctgc caaggtagca gagcatgcct
    15841 ttctgcaata taacctacag ccgacaacag gatcaataca gcagctattc ttttaattga
    15901 tctatcaggt atattcatct atccattgaa atataagttc atttaattac ctaacttttc
    15961 ctcgcttttt atacactaca taaattgttc gccgataaaa ccgaaaaata ttaatatctg
    16021 ttgtgtataa attatacata acaagatgtc attcatacga aagataagga aggcaagtgg
    16081 aac
//
LOCUS       DASRUK010000035         2120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_30849, whole genome shotgun sequence.
ACCESSION   DASRUK010000035 DASRUK010000000
VERSION     DASRUK010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30849"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..357)
                     /locus_tag="VFF28_01625"
     CDS             complement(103..357)
                     /locus_tag="VFF28_01625"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HZX44362.1"
                     /translation="MVLGIIAIVFGWIPFIGWLISIAGLALGIAALVAIHHHPEMEGR
                     GMAIAGIVLSGVAIIVYIFLAITIFAVFFKELAQRTGQLA"
     gene            complement(505..1932)
                     /gene="gatE"
                     /locus_tag="VFF28_01630"
     CDS             complement(505..1932)
                     /gene="gatE"
                     /locus_tag="VFF28_01630"
                     /inference="COORDINATES: protein motif:HMM:NF014930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HZX44363.1"
                     /translation="MDYQKLGFKCGIEIHQQIEGKKLFCDCPALNSTKEPDIFVERRL
                     RAVAGETGEIDVAAKHEMQKAKKFIYISNSEDTCLVDYDDEPPHPIHRQALDVALAVA
                     VALNAKVVDELQVMRKTVVDGSNTTGFQRTALVARNGFIDIPSGKVRISIICLEEEAA
                     QKVKEDEKSVTYKLDRLGMPLIEIGTAADIKSPDQAKEVAAYLGMVLRSTGSVKRGIG
                     TIRQDVNISIKGGARTEIKGFQDLRSIVKVIENEVVRQEELIKRGKEITSEVRKSEPD
                     FSTSFLRPMPGAARMYPETDVVPIKITQEMIDNVSKVELISEKIKQVASEYKMTSDLA
                     TGLFKAGKIAVFEQFAGKFKDLAPAFIASTMVSSVKEVERKHNLDGSKLLDKDLEHIF
                     SLINEGRVSKDSITDILLDTIKGVFDEKKYASASSEELEEEIKKIVINKPGLSMGGYM
                     GLVMAKFKGKVDGKKAMEILKKIVG"
BASE COUNT          561 a          532 c          360 g          667 t
ORIGIN      
        1 attagacaac ctccgtgtta tagtgtgaaa atcccacaaa aaacgggttt ttaggctgtt
       61 tataaatttt gctaaggcta ttggcagata cgtaaagaaa gattaagcaa gctgtcctgt
      121 cctttgggca agctccttaa aaaaaaccgc aaatatggtt atcgccaaaa atatatagac
      181 gattattgca acaccgctta atactatccc tgctatcgcc attccccttc cttccatctc
      241 aggatgatgg tgtatggcaa caagcgcagc gatccccagt gcaaggcctg caatggagat
      301 aagccatccg ataaatggta tccacccaaa gacaatagct atgatgccta agaccattga
      361 acttaaacct agacgtgaat gcagcaaaga aatcacctcc tgaatataaa aaatatgttt
      421 tttggatatt taatgatttg taattattac cttgtcacag aaattatagt ccgacatggc
      481 ttgttctcgc tttttgtgaa aaacctagcc gactattttc tttaatatct ccatcgcctt
      541 ctttccatcg acctttcctt tgaactttgc catgacaagc cccatatagc cgcccatgct
      601 caggccaggc ttgttgatga ctatcttctt tatctcttcc tcaagctcct cgcttgatgc
      661 agaagcatat ttcttttcat caaacactcc cttgatagta tccaaaagga tatcagtgat
      721 agaatccttg cttacccttc cttcatttat caaagaaaaa atgtgctcga gatccttatc
      781 caaaagcttt gagccatcta gattatgctt cctttccact tcctttacgc ttgagaccat
      841 tgtggaagcg atgaacgcag gagcaagatc cttgaatttg cctgcaaact gctcaaaaac
      901 agcgattttg ccagccttaa aaagccctgt ggcaaggtcg cttgtcatct tatattcaga
      961 agctacttgt ttaattttct ctgatatcaa ctctaccttg gaaacattat cgatcatctc
     1021 ttgagttatc tttataggca cgacatctgt ctcaggatac atccttgcag caccaggcat
     1081 cggcctaaga aacgaggtgc taaaatccgg ctctgatttt cttacctccg atgttatctc
     1141 ttttcctctt ttgataagct cttcctgccg tacaacctca ttctcgatga ccttgacaat
     1201 gcttctcagg tcctgaaaac cttttatttc agtccttgcc cctcctttga tggatatgtt
     1261 tacatcctgc cttattgttc ctatccctct cttgacactc cctgtgcttc taaggaccat
     1321 tccaagatag gctgcaacct cttttgcctg gtctgggctt tttatatcag ccgcagttcc
     1381 tatctctatc aaaggcatgc ccaatctatc aagcttgtaa gtgacagact tttcatcctc
     1441 ttttaccttc tgtgcagcct cttcttctag gcagattata gaaatcctca cttttccgga
     1501 aggaatgtcg ataaaaccgt ttcgtgcgac aagtgctgtc ctctgaaacc ctgtagtatt
     1561 ggatccatcg acgacagttt tcctcatcac ctgtagttcg tcgacaacct ttgcattgag
     1621 cgcaacagcg accgctaaag caacatctag cgcctgccta tggataggat gcggcggctc
     1681 atcatcatag tccacaaggc aggtgtcctc agaatttgag atatatatga acttctttgc
     1741 cttttgcatc tcatgctttg cagcgacatc tatctcccct gtctcaccag caacagcacg
     1801 aagccttctc tccacgaata tatcaggctc ttttgtagaa ttaagtgcag ggcagtcgca
     1861 gaatagtttc tttccttcga tctgctgatg gatctctatc ccgcatttga agcctagttt
     1921 ttgataatcc atatgcaatg caaaaattgg caagtataag tattttacta tctatgcctt
     1981 tgcagctttt cagcttgata atgagattcg ataaggatac cgactaaatt acccaaatct
     2041 tccataaaat cttcatcgat atcatcaaca tgaaatcttt tatcccttgg tctaccccta
     2101 attaccgcct gacctttatg
//
LOCUS       DASRUK010000036        10962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2042857, whole genome shotgun sequence.
ACCESSION   DASRUK010000036 DASRUK010000000
VERSION     DASRUK010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 10962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10962
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2042857"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..973
                     /locus_tag="VFF28_01635"
     CDS             158..973
                     /locus_tag="VFF28_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44364.1"
                     /translation="MKSEFPPDEQLSPHQLETRVKTSLEHDGNAQHIVKVIRGDKPEK
                     EVDNRGFMIYHIYKPQSGSPFALIEYTASVGDNKSLGYGTQLFNQVKKELARQGVEHL
                     VIETEMVPRVVENPVHTDLEDFTQTYGRTGDLWTISKGIGVWNGIDRRNHFWQERGFG
                     IVPDLAYCQPDLAEGKMERRVPLDAQVASLKGDGSEISAPRIRQILLAIAEHNYDLGR
                     FSTAFVDKQLQVTYSGKPITDNTIFEVIPYNKLLAKPEVSFALRMNSLKTRYQ"
     gene            complement(976..2145)
                     /locus_tag="VFF28_01640"
     CDS             complement(976..2145)
                     /locus_tag="VFF28_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005036165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HZX44365.1"
                     /translation="MQEVILKEKTALDKELMKKLAEEHGTPLFIIDHAKIRENYREFK
                     EHLPNVQAYFAVKSNSNPLIIKTLFEEGASFDVASMPEFLLVHENIKAMPEKERQDWI
                     WDKIIYANTIKPVETLQALDKYKPLVTYDNFEELKKIKENAPNAGLCLRIRVPNTGSM
                     VELSSKFGADPGEAVDLIEEAFKIGLIVEGISFHVGSQCTNFENFVQALNLSSSIIKE
                     VGSRLGKKINILDIGGGFPVKYNEKVKSFRTLARKINSEIKRLFPPGMEILAEPGRFM
                     IANACTLVAKVIGKAYRDGKPCYYINDGVYHTFSGQIFDHCVYPVKAIKEGEAKISAV
                     FGPTCDALDTITLAAELPELEIHDLVYAENIGAYSHASSTFFNGFSPAKVVHINL"
     gene            complement(2147..3610)
                     /locus_tag="VFF28_01645"
     CDS             complement(2147..3610)
                     /locus_tag="VFF28_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015410910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HZX44366.1"
                     /translation="MKLRFDKKILIIGYGAVSRCLLPILVSHLEAPFENVTIIDFEDK
                     AEAVKEWTAIGMSFVQIRITPENLAEVLSGYLEEKGMLIDLGWNIGCNDILDWCHKNN
                     VLYVNASVEQWDPYKDQEKKSAYEKSLYWRHMQLREMMKSWKEKGPTAVLDHGANPGL
                     ISHFVKQGLIDIASRLIKEKKVSEKEASDLKDLLRKRDFPRLAMKLDVKVIHCSERDT
                     QVSDKPKEPNQFVNTWSIAGFHEEGIGPAEMGWGTHERWMPASAVVPEVGPKNQIFLQ
                     QSGINTWVYSFVPPSHEIIGMVIRHGEAFTISDRLSVEENGKVVYRPTVHYAYNPSDA
                     AIASLNELRGLNYRLVEAQHIYNDTEIIKGGDILGALMMGHRYNSWWTGSDLSIEEAR
                     NLVPGQNATTIQVAAGTLAAIMWMIENPDRGVCLPDDLPHEYILNIAKPYLGKFISEP
                     FDWTPLKKRTILFPKNPSLVQPDRSDPWQFKNFYFSP"
     gene            complement(3708..4859)
                     /locus_tag="VFF28_01650"
     CDS             complement(3708..4859)
                     /locus_tag="VFF28_01650"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HZX44367.1"
                     /translation="MKGLVIVPGGFEDIGALELTELIGAKAPQVEDSCLLFDIKKIED
                     LCLLCYTAQSVEKVLLLIDNFQFKDYKDFEQKISGSIKKIDSKKWLGKTVAFRVSTKK
                     IGSDLSSEEVSARVGELIIESIKTYKQKVDLDNPDITFLVFINKDQAYLGIDFSGRDL
                     HKREYKIFTHHSALRPTIAYSLIRLAGYSNKKKLLDPFCCSGEIAIETALFQSNYSPN
                     FYSKDKFAFLKFKPLKKTDFAKLLGKQDKKILKIKKADVMCFDSNMLNLNAAKKNAKI
                     AGINKLIDFSRKDTEWLDTKLGKESVDLIVTHPPESSKVIGEKDIEKVYNEFFYQAEF
                     IVKKEGKVAVILTKSDLIKKAAAKYNFKPVHERKVYSGKAELSVVILKR"
     gene            complement(4872..8351)
                     /gene="gyrA"
                     /locus_tag="VFF28_01655"
     CDS             complement(4872..8351)
                     /gene="gyrA"
                     /locus_tag="VFF28_01655"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HZX44368.1"
                     /translation="MRYCVTGDTLLLTDSGIRHISQISSLDEQEIQLKILSLDGKKNT
                     ASKFFNSGFHPTIRLETNLGYAICGSYNHPILCWGNKDGFPCIEWKTLDTISDGDIVV
                     LSRGNGHFSKSSLKLNPFFPKEGFKNQIDLPKKMNDDLAFLLGALVSEGSFHNKQILF
                     NNKDITFYDKVKSLIFNQFEGIQIYERQVKGSCTELSIYEQKVVKFLKNIGLVEERSD
                     KKEIPFSILQSSKENIIHFLQALFEGDGSVVYKTDKRHNGKSIELTYNSKSLTLINQL
                     KVLLLNLGIVTTCPYKDKRNGCYKLIISGHDSIESFKQNAGFFSERKVDCLSHIDGMN
                     NLRLSKTDFVPYLSDYLRAKYKKEFIHKHNLDRYNLIKKNYAALVNLIDPKDKHLIDL
                     ILKRHYFFDTVKKISKTGIKQQVYSIRVDSGCHSFVANGFINHNTEARLKKLSEDILQ
                     DIDKETVKFVANFDGSLKEPSVLPSKVPNLLINGSSGIAVGMATNIPPHNLSEVAAGI
                     ISVIEDPDTSVEQIMQHIKGPDFPTGATICGSQGIKHAYKTGRGRVIVRAKAEVEEDR
                     ILIHEIPYMVNKAELIKQIAALVNDDKLTGISDIRDESDREGMRIVIELKKDANPNVV
                     LNQLYKHTRLQETFGIIMVALVNNEPKYLGIKEIIHYFIEHRKEIVRKRTEFDLNKAK
                     EKAHILEGLLKALADIDNVIALIKHSKSAENAKKALQESLLITEKQAAAILEMRLQRL
                     TALEQEKIKLEHAGLMKLIKELTEILASEKRILDIINQEMLDLKEEYGDDRRTMIEAA
                     EAEALEMEDLIKEEKTVITITHTGYIKRQPLAAYKQQKRGGKGIIAAGTKEEDFVENM
                     FIANTHSYLLFFTDKGRIHWLKVYNVPEGSRQALGKSIVNLLEIGQDEKISAFIPVAE
                     FKENEYLIMATRKGTVKKTELMAYSNPRKGGIIAITLDPGDELIEVVKTNGNNEIMLA
                     TKNGSAVRFKETDVRPTGRSSKGVIGATLRDNDEIVGMVVADDNNTVLTITNNGYGKR
                     TPVSEYRLTRRGGVGVKNILCSERNGCVVAVKTVKDNDELMFISRQGITIRTPANGIS
                     IIGRATQGMRLMKLDDNDSVVAAAKIIGEDVEVASEQASCTNDEDKDPVQGQAELEPQ
                     QNPDLDKGLNNEE"
     gene            complement(8343..8720)
                     /locus_tag="VFF28_01660"
                     /pseudo
     CDS             complement(8343..8720)
                     /locus_tag="VFF28_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009673409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
     gene            complement(9029..9964)
                     /gene="scpB"
                     /locus_tag="VFF28_01665"
     CDS             complement(9029..9964)
                     /gene="scpB"
                     /locus_tag="VFF28_01665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HZX44369.1"
                     /translation="MSNLKYKLEALLFSSARSMKIDELASLTKAGPEEIRETLVQLKA
                     DYEKRDSSIILIDEGDSFKLNIKSEHIQVIQQIVTETELSKTLMETLAVIAFKYPIKQ
                     SDLIKIRTNKAYDHLTQLEQMGYIIRQKFGRSKLIKLTPKFFEYFDLPPEKLKEKFQD
                     FSAIAKAIEDKEEEVKAIKQEIKRKAAESGKKEIGLVDDKGKAQALQIYDIPKEEEAA
                     QDLALYEEKIGELEVVDLPEEEEKKKPKREKKTAEASTAKNEQESEDRLPAPSGDGQE
                     PLDSENQETDEQPIEEEEQSEEKSKEEQTIEKKVG"
     gene            complement(9978..10550)
                     /locus_tag="VFF28_01670"
     CDS             complement(9978..10550)
                     /locus_tag="VFF28_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44370.1"
                     /translation="MAKPNQDMIRFRAILEVLGRPKEHVENTIKTLVAKAKERHDVSV
                     INEKFADIREEGKLFSTFVELEIVAKGTNNLSAFCFDFMPSSIEVDKPDQVILRNADL
                     SDVYNDLQAKLHTVDMIAKKLNAENDFLRRNLGTMISNLLTVLIKIGKNSLNDLSKFT
                     GINEKELADYIKKLEQEGKIKKEGDIFQLV"
     gene            complement(10586..>10962)
                     /locus_tag="VFF28_01675"
     CDS             complement(10586..>10962)
                     /locus_tag="VFF28_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44371.1"
                     /translation="ANKKTIFVKQGELAVAELTDKVISAEPGEYNLKVRLKRDDQKTE
                     KEITEVLEILPQTLTQTTGLKAVKKEVQEELNSSYFFRPLSYPKLIYESPQEKSKGLA
                     LYFFIGTLALYASFITFTKKPL"
BASE COUNT         2761 a         2402 c         2051 g         3748 t
ORIGIN      
        1 attggttttg tttcaagctc ctgcaggact gaaagcttga attcttttgt aaattttctt
       61 cttgttccca taaatcctca cctacgcttt ggctttatta aatttcgtta actaggtgtc
      121 caatttatgg ggcgcagtcc agtttcttgg ctatggtatg aagtctgaat ttcctcctga
      181 tgaacaactt tctccacacc agcttgaaac cagagtcaaa acctcattgg agcatgatgg
      241 caatgcccaa cacatcgtta aagtaatccg cggtgataag cctgagaagg aggtagacaa
      301 tagagggttt atgatatatc acatctataa accacagagc ggcagcccat ttgcactcat
      361 tgaatatact gcaagcgttg gagataataa aagcttggga tatggtaccc aattgtttaa
      421 tcaggttaaa aaagagttgg ccaggcaagg agtagaacat cttgttattg aaaccgaaat
      481 ggttccaaga gtagtagaaa atcccgtcca tactgaccta gaggatttta cccagactta
      541 tggtcgaaca ggtgacctat ggactatatc aaaagggatt ggtgtatgga acggcattga
      601 ccgtagaaat cacttctggc aagaaagagg atttggaata gtgcctgatc ttgcttattg
      661 ccagccagac ctagcagaag ggaagatgga aaggagggtg ccactagatg cgcaagtagc
      721 aagcctaaag ggagatggct cagaaatatc cgcccctagg ataaggcaga tacttcttgc
      781 aatagccgaa cataattatg acttaggaag attttcaaca gcatttgtag ataaacaact
      841 tcaggtgact tattcaggaa aaccaattac tgacaataca atatttgaag tcatacctta
      901 taacaagttg ttagcaaaac cagaggttag ttttgccttg aggatgaata gcttaaaaac
      961 aagatatcaa taaatctaca gattaatatg aacaaccttt gccggagaga atccattaaa
     1021 gaatgtagaa gatgcatgtg agtaagcgcc tatattctct gcatagacaa gatcatgtat
     1081 ctcaagctct ggaagctctg ctgcgagagt tattgtatcc aatgcgtcgc atgttggccc
     1141 aaagactgcg ctaatctttg cctcgccttc ttttatcgct tttacgggat atacgcagtg
     1201 gtcaaatatc tggcctgaga atgtatgata aacaccatca ttgatgtaat aacaaggctt
     1261 tccatccctg taagcttttc cgatcacttt tgcaactaag gtgcatgcat ttgctatcat
     1321 gaaccttcct ggctctgcca atatttccat gcctggaggg aaaagccgct ttatctctga
     1381 attaatcttt cttgcgagcg tcctaaaaga ttttaccttt tcattatact tgacaggaaa
     1441 gcctccgcca atgtccagga tatttatctt tttacccagc cttgagccta cttccttaat
     1501 tatgcttgac gaaaggttta gcgcctggac aaagttttca aagtttgtgc actggcttcc
     1561 cacatggaag cttattcctt ccacgattag gccgatcttg aacgcctctt caatgagatc
     1621 aactgcttca cctgggtctg ctccgaattt tgagcttaat tccaccatgg agcctgtatt
     1681 tgggaccctt atcctaaggc ataagcctgc gtttggagca ttctctttaa tcttttttag
     1741 ctcttcaaaa ttatcatagg tcacaagcgg cttatactta tccaatgcct gcagagtttc
     1801 cactggcttt atcgtatttg cataaattat cttatcccat atccagtcct gcctttcttt
     1861 ctccggcatt gccttgatat tttcatgaac aaggaggaac tccggcatcg atgccacgtc
     1921 aaagcttgct ccttcttcaa acaaggtctt tattatcaga ggatttgaat tggattttac
     1981 agcaaaataa gcctggacat tgggaagatg ctctttaaat tcgcggtaat tctcccttat
     2041 tttagcatgg tcaatgatga atagaggggt gccatgttct tctgctaatt ttttcatcaa
     2101 ctctttatca agagctgttt tttcctttaa gatgacttcc tgcattttag ggtgaaaaat
     2161 aaaagttttt gaactgccag gggtctgacc tgtcaggctg gaccaatgat ggattttttg
     2221 ggaacaagat ggtgcgcttc ttcagcggag tccaatcaaa cggctctgag ataaatttac
     2281 ccagataggg ttttgcgata ttcaatatat attcatgagg cagatcatca gggaggcata
     2341 cacccctatc tggattttca atcatccaca ttatcgctgc aagggttcct gctgcaacct
     2401 gaatggttgt cgcattctgg ccaggaacaa ggtttcttgc ctcttctatc gaaaggtcag
     2461 agcctgtcca ccatgagtta tatctatggc ccatcatgag tgcacctaag atatctccgc
     2521 cttttattat ctctgtatca ttatagatat gctgagcctc aacaagcctg tagtttaaac
     2581 cccttaactc atttaaggaa gcaatcgctg catctgatgg attatatgca taatggacag
     2641 tcggcctata tacaactttg ccgttttctt ctactgaaag cctgtctgag atagtgaatg
     2701 cttctccatg ccttatcacc atccctatta tctcatgact tggaggaaca aatgaatata
     2761 cccaggtgtt tatccctgac tgctggagga atatctggtt ttttgggcct acttcaggca
     2821 ctacagctga ggcaggcatc catctctcat gggttcccca gcccatctct gctgggccga
     2881 tgccttcttc atggaagccg gcaatgctcc atgtattaac aaactgattt ggttcttttg
     2941 gtttatcact tacctgcgtg tctctttctg agcagtggat aaccttgacg tcgagcttca
     3001 tagcaaggcg gggaaaatct ctttttctca acaggtcttt aagatcgctt gcttcttttt
     3061 cgctcacttt tttttcttta atgaggcggg atgcaatatc aatcaatccc tgctttacaa
     3121 aatgagatat aagacctggg tttgcgccat gatcaaggac tgcggtcggg cctttttctt
     3181 tccaggactt catcatctcc cttagctgca tgtgcctcca atataatgat ttttcatagg
     3241 cacttttctt ttcctggtcc ttgtatggat cccattgctc tactgatgcg tttacataaa
     3301 gaacgttatt tttgtgacac caatcaagaa tgtcgttgca gcctatgttc cagccaagat
     3361 caataagcat ccctttttct tcgagatacc ctgatagaac ttcagccagg ttttccggtg
     3421 tgatccttat ctgaacaaaa gacatgccga tcgctgtcca ttccttaaca gcttcagcct
     3481 tatcttcaaa gtcgatgata gtcacatttt cgaaaggcgc ttcaaggtga ctgaccaata
     3541 tggggagaag gcatcgtgag acagcgccgt acccgataat cagtatcttc ttatcaaatc
     3601 taagcttcat tatatcacag aaccagcttc tgatttattt ttcattgata tcgcaaattt
     3661 tatcaagcat ctgaaaacat aactaatatt taaaattttt cttacagcta tcttttgaga
     3721 atgacaaccg ataattctgc ctttcctgaa taaacttttc tttcgtgtac tggcttaaag
     3781 ttgtattttg ctgctgcttt ttttatcagg tctgactttg tcaaaataac tgcaaccttg
     3841 ccttcttttt tcacgataaa ttctgcctgg tagaaaaatt cattgtaaac tttttcaata
     3901 tctttttcac caatcacttt tgaagattct ggcgggtgag tgactatcaa atccacgctt
     3961 tccttgccca gctttgtatc aagccattct gtgtctttcc tggagaaatc gatgagcttg
     4021 tttatgcctg caatctttgc attctttttt gcagcattaa ggttgagcat gtttgaatca
     4081 aagcacatta catctgcttt cttaatcttt aggatcttct tatcctgctt gccgagtaac
     4141 tttgcgaagt ctgtcttctt gagcggtttg aattttaaga aagcaaactt gtcttttgag
     4201 taaaaattag gagaataatt tgactgaaac aatgctgtct ctattgcaat ctcgcctgag
     4261 cagcagaaag gatcaagcaa ttttttcttg tttgagtatc ctgccaatct tatcaatgaa
     4321 tatgcaatcg ttggccttag cgcactgtgg tgagtaaata tcttatattc ccgcttgtgc
     4381 aaatctcttc ctgagaaatc tatcccaaga tatgcctggt ctttgttgat gaagacaagg
     4441 aaggttatat cggggttatc gaggtcaact ttttgcttgt atgttttgat gctttctata
     4501 ataagttcgc ctactcttgc agagacttcc tcagagctta ggtcgcttcc tatcttcttt
     4561 gtggataccc tgaaggcgac tgtttttccc agccacttct tggagtcaat cttttttata
     4621 gagccagaaa ttttctgctc aaaatcctta tagtccttaa attggaaatt atctatcaga
     4681 agaaggactt tttcaaccga ttgggctgta taacaaagca agcaaaggtc ctcaattttt
     4741 tttatatcga acaaaaggca gctgtcctcg acctgaggtg cttttgcgcc gatcagctct
     4801 gttagttcca gagcaccgat gtcttcaaag ccgcccggaa cgatgaccaa acctttcata
     4861 gaaaaagtaa ttcactcttc attatttaaa cctttatcta aatcagggtt ttgctgcggt
     4921 tctaactctg cttgcccttg cacaggatct ttatcttcat cgtttgtgca ggaagcctgt
     4981 tcagaggcaa cttcaacgtc ttcacctatt atttttgctg cggcaactac tgaatcatta
     5041 tcatcaagct tcatgagcct catgccttga gtcgcccttc ctattatcga aattccattt
     5101 gcaggcgtcc tgatagttat cccctgcctg ctgataaaca taagctcgtc gttgtccttg
     5161 acagtcttta ctgcaacaac gcatccattc ctttctgagc ataaaatatt ttttactcca
     5221 actccgcctc ttcttgtaag cctgtattct gagacaggcg tcctttttcc ataaccattg
     5281 ttagttatcg tcagaacggt attattatca tcagccacga ccatgcctac tatctcgtca
     5341 ttatccctaa gggttgcacc tataactcct tttgagcttc ttcctgtagg ccttacatct
     5401 gtctccttaa agcggacagc tgagccgttt tttgtggcaa gcattatctc gttatttcca
     5461 tttgtcttga caacttctat aagctcatct cctgggtcta gagtgattgc gatgatccct
     5521 ccttttctgg gattggagta agccataagc tcggtttttt ttactgtgcc ttttcttgtg
     5581 gccattatca aatattcatt ttccttaaac tctgcaaccg gtatgaatgc tgaaatcttt
     5641 tcatcctggc ctatctccaa aaggtttaca atagattttc caagagcctg cctggagcct
     5701 tctggaacat tgtagacctt gagccagtgg atccttccct tatctgtgaa gaaaaggaga
     5761 tatgagtggg tatttgctat gaacatgttt tcgacaaagt cctcttcttt tgtccctgct
     5821 gcaataatcc cttttcctcc tcttttctgc tgcttgtatg ctgccagggg ctgtcttttg
     5881 atatagcctg tgtgagttat ggttatgact gttttttctt cttttatcag gtcttccatc
     5941 tcaagggctt ctgcctcagc tgcctctatc atggtccttc tatcatcacc atactcttct
     6001 tttaagtcaa gcatctcctg attgatgata tcaagtatcc ttttttctga ggccaagatc
     6061 tcagtcagtt cttttatcag cttcattagg cctgcatgct ctaactttat cttttcctgc
     6121 tcaagggcag taagcctctg aaggcgcatc tcaagaatag ctgccgcctg tttttcagtt
     6181 atcaacaatg actcctgcaa tgcttttttt gcattttctg ctgacttgct atgctttatt
     6241 agcgctatta cgttgtcaat atcggccaat gccttcagga ggccttcaag gatatgcgct
     6301 ttttcctttg ccttgtttag gtcaaactca gtcctttttc ttactatctc cttcctgtgt
     6361 tcgataaaat agtggattat ctccttaata cccaggtatt ttggctcatt gttgacaaga
     6421 gcaaccatta ttatgccaaa tgtctcttga agccttgtgt gcttataaag ctggtttaga
     6481 acaacgttgg gatttgcgtc tttttttagc tcaattacta tcctcatacc ttccctatct
     6541 gattcatccc ttatgtcgct tatccctgtc agcttatcat cgttaaccaa agcagctatc
     6601 tgttttatca gctctgcctt attgaccatg tatggaatct catggatgag tatcctgtct
     6661 tcttctacct ctgctttcgc acggacaatg actcttcccc tgccagtctt ataagcatgc
     6721 tttatgccct gggagccaca gatagtcgcc cctgtgggaa aatcaggacc ttttatgtgc
     6781 tgcattatct gctctacgct ggtgtctggg tcttcaataa cagatatgat tcctgctgca
     6841 acctctgata agttatgagg cgggatgtta gttgccatac caactgcaat gcctgacgaa
     6901 ccgttgataa gaaggtttgg aacttttgaa ggaaggacag aaggctcttt taggctgccg
     6961 tcgaagtttg caacaaactt tactgtctct ttatcgatgt cctggagaat atcttccgag
     7021 agttttttta ggcgggcctc tgtattatga ttaatgaacc cgttggcaac aaatgagtga
     7081 cagccactat ctaccctaat agaataaact tgctgtttta tcccagtttt gctgattttt
     7141 tttacggtat caaaaaaata gtgacgctta aggattaaat cgataaggtg cttatccttt
     7201 ggatcgatta gattgacaag agctgcataa ttctttttta tcaggttata tctatccagg
     7261 ttatgcttat gtatgaactc ttttttgtat tttgccctta aataatcact tagatatggg
     7321 acaaagtctg ttttactcag cctgaggtta ttcatcccat cgatatggga taagcaatct
     7381 accttccttt cggaaaaaaa tccggcattt tgcttaaagc tctctattga gtcatggcct
     7441 gaaattatta atttgtaaca tccgttcctt ttgtccttat acggacaggt ggtgactatc
     7501 cccaaattta ataacagcac tttaagctgg ttgataagcg tcaaactttt ggaattatat
     7561 gtgagctcaa tagacttacc gttatgcctt ttatctgtct tatatacgac tgaaccatct
     7621 ccttcaaata atgcctgcag gaaatgtatt atgttctcct ttgatgactg caagatagaa
     7681 aaaggtattt ccttcttatc tgacctttcc tcaacaagcc caatattttt taggaactta
     7741 acgacttttt gttcatatat acttaactca gtgcatgagc cttttacttg cctttcatag
     7801 atctggatac cttcaaattg gttgaaaata agggacttaa ccttgtcgta aaaagtgatg
     7861 tctttgttat tgaacaaaat ttgtttgttg tgaaaactcc cctcgctaac aagggcgcca
     7921 agtaaaaaag ctagatcatc attcattttt tttggaagat ctatctgatt cttgaaaccc
     7981 tcttttggga aaaatggatt aagcttaaga gaagatttgg aaaaatggcc attccccctg
     8041 cttaatacca caatatctcc atcggatata gtgtcaagtg tcttccattc tatgcatgga
     8101 aaaccatcct tatttcccca gcacagtatt ggatggttgt atgagccgca gatagcataa
     8161 cccagattag tctctaacct tattgtgggg tgaaagcctg agttgaagaa cttgctggcg
     8221 gtgttttttt ttccgtccaa tgataggatt ttaagttgta tttcctgttc gtcaagcgag
     8281 gatatttggc ttatatgcct tattccagaa tctgtaagca ataatgtatc ccctgtgacg
     8341 caatatctca tcgcggccgg tgagtcaccg tcgacactac caaagtttcc ctggccgtct
     8401 ataagcctgt atctaagcga ccagtcttgc gccatcctta ccatagaatc atacactgct
     8461 gtatcaccat gaggatgata cttgcccaga acctctccaa caatccttgc gcttttttta
     8521 aagggcttgt tatgaagcat gcccatatcg ttcatggcaa aaagaatcct tcggtgaact
     8581 ggctttaggc cgtcccttac gtctggaagg gcccgtccaa ctatgacaga catagcatag
     8641 tccacatagg actgcttcat ctcttcttct atctctctgg gtattatttc ttctccttcc
     8701 agtgttattt tatcgaccat tttattagaa agttatatta tatttgaaca gaggttcatt
     8761 tataaatctt actataaaat tcaggcttta taaggcgtat aagggctaaa ttttgggttt
     8821 ttaggtagac gagaaaacaa acccaaaccg cagcaagcta tggagtatga actttagttt
     8881 gagcaatcat ttatgaggtt atctctaacc ttgtagcttt tattacagta aacgcatgtt
     8941 tttcttttac ctgtaaggat agaatctttt gattgatatt tcatggtatt tttgcagttt
     9001 gggcacttta acaaaagggt tttcatcttt agcctacctt tttttctatc gtttgctctt
     9061 cttttgactt ctcttcagat tgctcttctt cttcgattgg ttgttcgtcg gtttcttgat
     9121 tttcggaatc caggggttct tgtccgtcac ctgatggagc tggtaatctg tcttcagact
     9181 cttgctcgtt tttggctgtg ctggcttctg cagttttctt ttctctcttg ggctttttct
     9241 tttcttcctc ttcaggcaga tcgacaacct ccagctctcc aatcttttct tcatagagtg
     9301 caaggtcttg tgctgcttcc tcttcctttg gaatatcata aatttgcagt gcctgagctt
     9361 ttcctttatc atcaacaagg cctatctctt tttttcctga ttccgctgct tttcttttta
     9421 tctcctgctt gattgccttt acttcctctt ccttatcctc tatcgctttt gcaatagcac
     9481 taaaatcctg gaatttttct ttgagctttt ctggcggaag gtcgaaatat tcgaagaatt
     9541 ttggagtaag cttgataagc ttactacggc caaacttctg ccttataata tatcccatct
     9601 gctcaagctg ggtaagatgg tcatatgctt tgtttgtcct tatctttatc aaatctgact
     9661 gctttatggg atacttaaat gctattacag ccaaagtctc catcaaggtc ttacttagtt
     9721 cagtctctgt aactatctgc tggataacct gaatatgctc tgatttgata ttaagcttga
     9781 atgagtctcc ttcatcgatg aggataatag agctgtcacg tttttcatag tcagccttta
     9841 gctgaaccaa tgtttccctt atctcttcag ggcctgcttt ggtaagggat gccaattcgt
     9901 caattttcat acttctggca gaagaaaaaa gcaatgcttc taatttatac ttaagattag
     9961 acatggatca ccctctttta tactagctga aagatatcgc cttctttttt aatctttcct
    10021 tcctgctcaa gcttttttat gtaatctgca agctcttttt catttatccc ggtaaacttt
    10081 gacaaatcgt tcaaactatt cttccctatc tttatcagca ctgtcaggag attggatatc
    10141 atcgttccca ggtttcttct aagaaaatca ttctctgcat ttagcttctt tgctatcata
    10201 tcgacagtat gcagctttgc ctgcaaatcg ttataaacgt ctgataagtc tgcatttcgc
    10261 aggataacct ggtctggctt gtctacctcg atagatgaag gcatgaaatc aaagcagaat
    10321 gctgacaaat tgtttgtgcc ctttgccaca atctcaagtt caacaaatgt ggagaaaagc
    10381 tttccttcct cacggatgtc tgcaaatttt tcgtttatga ccgaaacatc atgcctttct
    10441 ttggcctttg caaccaaggt ttttatcgta ttttcaacat gctcctttgg cctgccaaga
    10501 acttccagaa tcgccctgaa cctgatcata tcctgatttg gttttgccat aggacaaagt
    10561 gatttccaat cctataaaaa cgtttctata gtggtttttt tgtgaatgtg atgaaagagg
    10621 catatagcgc aagggttccg ataaaaaagt agagggcaag cccttttgac ttttcttgcg
    10681 gcgactcata aatcaacttg ggataagaca aaggtcggaa aaagtaagag ctgttcaatt
    10741 cctcctggac ttcctttttt actgctttta gcccggttgt ttgagtaagg gtttgaggca
    10801 gaatttccaa gacttcagta atctccttct ctgttttttg gtcatctctt ttgagcctga
    10861 ccttcaggtt atactcgcca ggctctgcac tgataacctt atcggttaat tctgcaaccg
    10921 ctaattctcc ttgttttaca aatatcgtct tcttattggc tt
//
LOCUS       DASRUK010000037         5418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1207817, whole genome shotgun sequence.
ACCESSION   DASRUK010000037 DASRUK010000000
VERSION     DASRUK010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 5418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5418
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207817"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(349..735)
                     /locus_tag="VFF28_01680"
     CDS             complement(349..735)
                     /locus_tag="VFF28_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44372.1"
                     /translation="MTLSAKEERILKTIVVASENDPSKPEFPADNPKFPATPIYKIDV
                     PGFSNVWLKDESHNPTGTHKDRMAWEIVVTYRDFLLAKKRGQIKGHLPSIPQTPTNLL
                     AIQKLSLTKPLNEVTNFPKRPLGRSY"
     gene            complement(775..1020)
                     /locus_tag="VFF28_01685"
     CDS             complement(775..1020)
                     /locus_tag="VFF28_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44373.1"
                     /translation="MTDKKLLIAEHRDLVEALYNLAKSQIVLAKAIRVIGSDSSMPAS
                     VSNYIYDKEKQFKRIITKFSRQAKELQCLLKIEESQQ"
     gene            complement(1025..1549)
                     /locus_tag="VFF28_01690"
     CDS             complement(1025..1549)
                     /locus_tag="VFF28_01690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZPR1 zinc finger domain-containing protein"
                     /protein_id="HZX44374.1"
                     /translation="MADEMDVMEGETCPICQQKTLTLTDAEKEIPYFGKVYLYSMDCS
                     NCNYHKADVESVEQHEPAKYTFEVTKEEDMKVRVVKSSEATIKIPHITTITPGPASSG
                     YVTNIEGILSRVKKQVEVARDTAEDDEDRKKAKNLVKKLINIMWGRQPQKIIIEDPSG
                     NSAIISDKAVKGRL"
     gene            1645..2307
                     /locus_tag="VFF28_01695"
     CDS             1645..2307
                     /locus_tag="VFF28_01695"
                     /inference="COORDINATES: protein motif:HMM:NF020022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZX44375.1"
                     /translation="MFLSEIYSKNWTGIISQENILSFPHPKDEMLLPYSRLSITRIPS
                     IPKNIESIQPKALAELIDSKGITHSHLSKNYWTLNEPIDKKKGFACHMMIGIPGSGKT
                     TLSQKLFRGLHLVSRDLHGRYAEEKLLKQFLSEGKSVVIDDTNYNKKTRQNLINLIKP
                     YKASIKGIYVYARLVACLERNIKRKKAIPAVGLRGLANKFETPSIDEGFDSLQLVNND
                     TV"
     gene            complement(2304..3584)
                     /locus_tag="VFF28_01700"
     CDS             complement(2304..3584)
                     /locus_tag="VFF28_01700"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HZX44376.1"
                     /translation="MENELFSMVEGYDANSRSIRLISQAAPLVGMDEERILEFIQPQD
                     IRVYRLHVKLFGKIKSIWGCTSLHNRSRGAYKGGIRIAPDVDVFETVELSRLMTIKTA
                     CVGLEFGGAKTGIRFDMAKAYQEFGKKPYDPEFEANIKREILHEFSHLFKPALVGHVY
                     IPAPDIGTNAIDMAAIYNETNDPSSVTGKPEGMPGWLPGRNEATGYGVAECTQRLLDG
                     LSLDVKDAQVAIQGFGNVGMHSAMFLAEKGASVVAISDKNASLYDEEGLDIPKLSAYA
                     LQNGTIKGFPGKEIGNIFGADCDVLIPAAISNSINIDNVDSIKAKGIVEAANMPIDYK
                     AEKRLAEKKVKIIPDIIANSGGVIASGEEFSKSLSARYMKKQDTYQMITNRIMENLDE
                     AMKISADSNISLNLACYALALKRIYSAHRIRGWA"
     gene            3717..4010
                     /locus_tag="VFF28_01705"
     CDS             3717..4010
                     /locus_tag="VFF28_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44377.1"
                     /translation="MDKILLEIKKTEIDSAKAIAEARHKAEEEVKTAKSDCEKILKQA
                     LHEQERLAKERIDEEARMLSLEKEKALAGVVQKEANIKKAAESIVNFFEQETK"
     gene            4007..>5418
                     /locus_tag="VFF28_01710"
     CDS             4007..>5418
                     /locus_tag="VFF28_01710"
                     /inference="COORDINATES: protein motif:HMM:NF013647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HZX44378.1"
                     /translation="MIRDMTKLRIIGPKTRMPDIISDLHKLKVMHIIEGQSDEANQGK
                     PMMEAEQLSVSLIKLRAVISQLKIKPSEEHKPEKLAKIIKTVDFIAKTLKAANESLKS
                     ADEIEKKGNTIIAHLNVLKALDIKEKTEFETIDVFVGYIKDAVKFKESLLKKTDRFLL
                     KTVNGAISLFIDKRFSDDAGHILSSNQFSQIPLTFEDRNEVLQAISKAKTDRKEALIK
                     IDLIRKTYADILPSYERMLSQELTKAEAPLKFIETDEAFLIWGFAPKARAQSMIQSLM
                     HRAHNNLFIEEREIEKYDQIPVSLDNPRKVKDFESLLSLYSLPKYKEIDPSWFLFLTF
                     PLLFGFMLGDMGYGITTAILFYILKKKMPNAGGFFNVLILSSFASIFFGIVFGEFFGL
                     ELYHPLLNRNPEHGLEPLMITAIAVGVLHINAGLIAGFINEFRAHGLKAAIFEKFSWI
                     VLEISVAVLALSYTQILSKV"
BASE COUNT         1558 a         1185 c         1069 g         1606 t
ORIGIN      
        1 aggcgccttc aagccatgct gcaacttcaa aacggccaat atagtctgca actgtagcca
       61 cagcattaac taatggatca tcttttaaat gatctggcaa tcgaattctc atgccatatg
      121 agaatatgga ggtattttta aagatttcta catttactac taaaaagtta agggttcacc
      181 gacaaaaaac ttttcggtcg aaatccgcca gaacttatgt aactggcgga gagtttaacg
      241 agccattaaa cagttcggga gcaccgcttc gctacttttc caagaaaagt tgctccctct
      301 cccaaatttc catcctgagc cccactctgg gctcgtttta aactccgttt aataacttcg
      361 cccgaggggt cttttgggga aatttgtgac ttcgtttaac ggctttgtta aactcaattt
      421 ttgaattgct aataagttag ttggggtctg aggtatgctg ggcaaatgcc cttttatctg
      481 gcctcttttt ttggcaagca gaaaatccct gtaagtgaca actatctccc aggccatcct
      541 atccttgtga gtgcctgtcg gattatggct ctcatccttc agccatacat tggaaaatcc
      601 aggaacgtct atcttatata ttggggttgc tggaaactta gggttgtctg caggaaactc
      661 aggcttgctt ggatcattct cagatgccac aactattgtt tttagtatcc tctcttcctt
      721 ggcgcttaaa gtcatagcaa tgacctagaa atatatctta tataagcctt tcgactattg
      781 ctggctttct tcaatcttca gcagacactg aagctctttt gcttgtctag aaaacttagt
      841 tattatcctc ttgaattgct tttctttatc atatatataa ttggacacgc tagcaggcat
      901 gcttgaatcg ctgcctatca cccggattgc ctttgctaat acaatctgtg attttgccag
      961 attgtacaac gcttccacta aatctctatg ctcagcaatc aagagttttt tgtcggtcat
     1021 agagttataa tctacctttc acagccttat cgcttattat cgcagaattc ccacttggat
     1081 cttcgatgat tatcttctga ggctgcctgc cccacatgat atttatcaat ttcttaacaa
     1141 ggttctttgc ttttttccta tcttcatcat cttcagcagt gtctcttgcg acctcaacct
     1201 gcttttttac cctgcttagt attccttcaa tgtttgtaac atagccgctt gatgcaggcc
     1261 caggggttat tgttgttata tgcggaatct tgattgtcgc ttcggaactc tttacaaccc
     1321 ttactttcat gtcttcttcc tttgttacct caaaggtata cttggcgggc tcatgctgct
     1381 cgactgattc aacatctgcc ttgtggtaat tgcagtttga gcagtccata gaataaaggt
     1441 aaactttgcc aaagtaaggt atctcttttt ctgcgtcggt cagcgtcaaa gtcttctgct
     1501 ggcatatagg gcaggtctcg ccttccataa catccatttc atcagccata gccctttaag
     1561 agagggataa ggtttttata ctttgcggtt tgatccaatt tttgattact ctatatttat
     1621 ttacagtgct gacttaatat gcctatgttt ctttcagaga tctacagcaa aaattggaca
     1681 ggcatcatct cgcaagaaaa catcttatcc tttcctcacc caaaagatga gatgcttttg
     1741 ccatattcca gattatcgat aactagaatt ccaagtattc caaaaaatat agagagcata
     1801 caacctaaag cattagcaga acttattgac agcaaaggca taactcacag ccacctctca
     1861 aagaattatt ggaccctaaa cgaacctatc gacaaaaaga aaggcttcgc ctgccatatg
     1921 atgataggca ttcctggctc aggcaaaaca acactctcac aaaaactttt ccgaggcctc
     1981 catttggtca gcagggattt gcatggaaga tatgccgaag agaaactcct taagcaattc
     2041 ttgtctgaag gaaaaagtgt agttatagac gatactaact acaataaaaa aacaagacaa
     2101 aacctgataa atctcatcaa gccttacaaa gcaagtatca aaggcatata tgtctatgca
     2161 aggctcgtag catgtcttga aagaaacata aaaagaaaaa aggcaatacc tgcagtagga
     2221 ctaagaggcc ttgcaaacaa gtttgaaacc cccagcatcg atgaagggtt tgatagcctg
     2281 caactcgtca acaacgatac tgtttaagcc cagcccctta tcctgtgcgc gctatatatc
     2341 ctctttagcg caagagcata gcatgcaaga ttaagggata tattgctgtc tgccgaaatc
     2401 ttcatcgctt catcaaggtt ttccattatc ctgtttgtta tcatctgata ggtatcctgc
     2461 tttttcatat atcgtgcaga aaggcttttt gaaaattcct cgcctgatgc aatgacaccg
     2521 cctgagtttg cgatgatgtc agggattatc tttacctttt tctcagcaag ccttttctct
     2581 gctttataat cgataggcat gtttgccgct tcaactatcc ccttggcttt tatactgtca
     2641 acattgtcaa tatttatgga attgctgata gctgcaggta tcagcacatc acaatcagcg
     2701 ccaaatatat tgcctatctc ttttcctggg aatcctttta tcgtcccgtt ttgaagcgcg
     2761 taggccgaaa gcttaggtat gtcaagccct tcttcgtcat aaagagaagc gttcttatca
     2821 gatatagcga cgacactcgc acccttctca gcaaggaaca tcgcagaatg catgccgacg
     2881 tttccaaatc cctgtattgc cacctgggca tctttgacgt caagagaaag cccgtcaagc
     2941 aaccgctgag tgcattcagc aacgccatat cctgtggctt cattcctccc tggcagccat
     3001 ccaggcatgc cctctggctt acctgtgacg gatgatgggt cgttagtctc attgtatatt
     3061 gcagccatat cgatagcatt agtgcctatg tctggtgcag gtatatacac atggcctacc
     3121 aaggctggct tgaatagatg tgaaaattca tgaagtatct cgcgcttgat atttgcctca
     3181 aactcaggat catatggctt cttgccaaat tcctggtagg cttttgccat gtcaaacctt
     3241 atgcctgtct ttgcgccgcc aaactcaagg ccgacgcatg ctgttttgat cgtcataagc
     3301 cggcttaact ctactgtctc aaaaacgtcg acatcagggg ctatcctgat acctcccttg
     3361 tatgctcctc ttgacctgtt atgaagagac gtgcagcccc agatgctttt aattttacca
     3421 aaaagcttca cgtgaagcct atatactctt atatcctgag gctggatgaa ctcaagtatt
     3481 ctttcctcat ccataccgac gagcggcgca gcctggctta taagcctgat agaccttgaa
     3541 ttagcgtcgt atccttcaac catgctaaaa agttcgtttt ccatatttac acctctgttg
     3601 gtaattaaaa cgcttgggta aggcttgtgg taattaaatc tttctgtttg aagttatcaa
     3661 ttgttaacca aaaaaataaa ttttataaag ggatgagtaa cccgtttatt gtttctatgg
     3721 acaaaatcct gctcgagatt aaaaaaacag agatagactc agctaaagca atagcagagg
     3781 ccaggcataa ggcagaggaa gaggtaaaaa cggcaaaaag tgattgtgaa aaaatcttaa
     3841 agcaggcttt gcatgaacag gagaggctgg caaaagaaag gatagatgag gaagcaagga
     3901 tgctttcttt agagaaagaa aaggctcttg ctggtgttgt ccaaaaagaa gccaatataa
     3961 aaaaagccgc tgaatctata gtcaacttct ttgaacaaga gacaaaatga tcagagacat
     4021 gaccaagctt aggataatag ggccaaagac ccgtatgcct gatatcatct ctgatctgca
     4081 taaattgaaa gttatgcaca tcatcgaggg gcaaagcgat gaggcaaacc aaggcaagcc
     4141 tatgatggaa gctgagcagc tttctgtatc tcttatcaag cttagggctg tgatatcaca
     4201 gctaaagata aagccttctg aagaacataa gcctgaaaaa cttgcaaaga taataaagac
     4261 agtagacttt atcgcaaaga cgctcaaggc tgcaaacgag tctttaaagt cagccgatga
     4321 gatagaaaaa aagggcaaca cgattattgc tcatctgaat gttctcaaag cgctcgacat
     4381 aaaagaaaag accgagtttg aaaccatcga tgtctttgtg ggttacatca aagacgctgt
     4441 caaattcaag gagagtcttc ttaagaagac agacaggttt ctccttaaaa ctgtcaacgg
     4501 cgcaatctct ctttttatcg acaaaagatt ctcagatgat gccgggcata tcttgtcatc
     4561 caaccagttc agccagatac ctctaacttt tgaagataga aacgaagtgc ttcaggcaat
     4621 ttctaaggca aaaaccgaca ggaaggaagc gctgataaag atagacctga taaggaaaac
     4681 ttacgcagat atccttccgt cttatgagcg gatgctttca caagagctta ccaaagccga
     4741 ggctccttta aaattcattg agacagatga ggcatttctt atatggggat ttgcgccaaa
     4801 agccagggca cagtcaatga tacaatccct gatgcatagg gcacacaata atctatttat
     4861 agaagaaagg gagatagaaa aatatgacca gatcccagtg tctttggaca acccaagaaa
     4921 agttaaggac tttgaatctt tattaagcct ttattccctt ccaaaataca aggaaataga
     4981 tccttcgtgg tttttgttcc tgacttttcc gctgctattt ggctttatgc ttggtgatat
     5041 gggctatgga attactactg caatactttt ctatatcctg aaaaagaaga tgcctaatgc
     5101 agggggattt tttaacgtac tgatcctgag ctcttttgct tcgatatttt ttggaatagt
     5161 ctttggagaa ttttttgggc ttgagcttta tcatcctttg ctaaacagga atccagagca
     5221 tggacttgag ccgctgatga taactgctat agctgtcggc gtacttcata tcaatgccgg
     5281 ccttatcgca ggattcatca atgaatttag ggcgcatggc cttaaggctg ccatctttga
     5341 aaaatttagc tggatagtgc ttgagatatc tgtcgctgtc cttgcgcttt cttatactca
     5401 aatactttct aaagtctg
//
LOCUS       DASRUK010000038         7966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_376230, whole genome shotgun sequence.
ACCESSION   DASRUK010000038 DASRUK010000000
VERSION     DASRUK010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 7966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7966
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376230"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VFF28_01715"
     CDS             complement(<1..282)
                     /locus_tag="VFF28_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44379.1"
                     /translation="MAIIAELNEFRKIYGQGFIKGVFTGIDNIMLYLGLIKKSRGPLA
                     IGWDVTFNCNARCSFCDVWKMKGKGKELTTKECIGIIRQAGRAKTWAISF"
     gene            complement(273..1439)
                     /locus_tag="VFF28_01720"
     CDS             complement(273..1439)
                     /locus_tag="VFF28_01720"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZX44380.1"
                     /translation="MKYRLLDLIVCPYCSKRLSLKVLAEKKNHYLVSKKNRCKICTFK
                     KKEACKECFNTEITEAILECSCRRIYPVIRGIPRMLPDDFMNDFLEKEVDFAERYRHY
                     LKGSNRKIKDDHKNIKKSTVERFGYEWKNFSKLYESYENQFLDWIYPVNQHFFAGKLV
                     LDAGCGTGRHAYYSAKYGAETVAFDFSDSVEVAYKNTLKFPLVHIIHADIYNMPFRAG
                     RFDYAYCIGVLHHLPGPKQAFDNLVSKIKKGGTISVWVYGKEGNYLLKLMDPIRKQVI
                     SKLPIGLTYKLSFIAMLGIHPLTKLIYKPMNSSKSTRKIAEKILPQNSFFNYLGNFKF
                     EQNHSILFDQLLAPIAFYYTKQEFERWFDKPCLSDVKITWRNKNSWRGMAVKWQ"
     gene            complement(1458..3398)
                     /gene="asnB"
                     /locus_tag="VFF28_01725"
     CDS             complement(1458..3398)
                     /gene="asnB"
                     /locus_tag="VFF28_01725"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZX44381.1"
                     /translation="MCGICGFAGMEDGRFLKDMVRSISHRGPDAEGFFIDKHCSLGHR
                     RLSIIDIESGKQPMHNEDESIWIVFNGEIYNFKEIRDNLGKHRFYTNSDTEVILHAYE
                     EYGPECLKKFNGCFSMAIWDSAKKRLFLARDRLGIRPLYYALVGDALVFASEIKAIVQ
                     NPQIKRKVNPKALHDFLTFRCVSTEETMFEGIMKLPPAHYIIYEDNKLKKTRYWSLDY
                     NRHGKVDENVISKNLLAALKKSINYRLMSEVPLGAYLSGGIDSSAIVAIMSELSDEPV
                     KTLSVGFDIEGYDNELKYARIVAEKFGTDHCEIMVKPDASRLFPKIIWHLDEPMSDPT
                     VIPTYLLASKTRPKCVVVLTGEGGDENFGGYEQYKMMKLHNTYARVIPKFIRRGAYGI
                     AAALPAKFLNRIFKYSSALGEEGLKRFREFITTDDYAKMYLSLVSIFNESEKKCILSD
                     EYKEATKSVDLVGALDASYFAQTTGKSLLDSLTSLEIDHILPENLLMKTDKMSMAFGI
                     EARVPFLDHNFVEFAATIPQSLKLKGFNEKYILRKAVSAKLPKLILQRKKQRFFVPID
                     SWFKGDLKGLMGQALSRQSVRESNAFSYDAIEKIFNNFDKSRLFYARQLWSLFAFQVW
                     YKIYIHNDPKKPILDLDRLISS"
     gene            complement(3407..4360)
                     /locus_tag="VFF28_01730"
     CDS             complement(3407..4360)
                     /locus_tag="VFF28_01730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZX44382.1"
                     /translation="MVHEVELSIVIPAYNEADSIPQVYQELKGVLDSLQKESEIIFID
                     DGSSDSTFSVLEGLHNKDPSVKVIRFRRNFGQTAALQAGFSASKGKLIVTMDADLQND
                     PKDIPRLLERQKQGYDAVSGWRSKRKDNFSKRFFSKIAEYIRRLVIKDYVHDSGCSLK
                     VYTKESIKGLALFGEMHRFIPAILVLNGFKVGELKVNHQPRLHGKTKYGIARLLKGLL
                     DLVYIKFWSSFSTRPLHFFGLLGFFQIVAGVMLAFANLSYHSIRYGALALGIGPVLLF
                     AGILIILGVQFIVLGFLGEILIRTYYKDAKDKGYVIEKVLG"
     gene            complement(4408..4563)
                     /locus_tag="VFF28_01735"
     CDS             complement(4408..4563)
                     /locus_tag="VFF28_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009727248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HZX44383.1"
                     /translation="MMIQDNLFKVLACPSCKASLKYGKDKTTLVCVKCAATYPIRKGI
                     PVLLPLS"
     gene            complement(4547..5542)
                     /locus_tag="VFF28_01740"
     CDS             complement(4547..5542)
                     /locus_tag="VFF28_01740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HZX44384.1"
                     /translation="MNKAIFFDRDGVLNKDPGYVHKLQDFCLFPGAVDALKRVKGFLF
                     FIVSNQSGIGRGLYTDEDFHRFNNHLLFELKKHGLHFSKVYYCPHSPEDKCDCRKPKT
                     KFIMDAKAKNNLNLKHSWVIGDHASDIKLGKNASCKTIHLLTGHGTKELAEVRKYKPD
                     YVAAGLGQATDFIMFNRRRKIVSRGEITRIADEQRKEGKTIVTINGTFDILHKGHEFI
                     LKSAKKQGDMLIVGLNTDSSVKQNKGPGRPINGEKARAMMLANFPFVDYVVLFDEKTP
                     IELLEEIRPDVHVNGSEYGKECIEAPTVKKHGGKIYIVGRIQGFSTTRLINDDTR"
     gene            complement(5520..6518)
                     /gene="rfaE1"
                     /locus_tag="VFF28_01745"
     CDS             complement(5520..6518)
                     /gene="rfaE1"
                     /locus_tag="VFF28_01745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HZX44385.1"
                     /translation="MRDELIKITEKFEGKRILVIGDIMLDKYIWGNVTRISPEAPVQV
                     VHVEKQSVVLGGAANTANNVASLKGTVSIIGVTGEDDARDRLIDELKKKGIMIDGIMS
                     EKNRQTIQKVRIMAQNQQLLRVDYEDGVLVSKEAEGKMIHFLKKSIDGFDGVVISDYF
                     KGIVTKDLIKEASSLIKAHGKFLIIDPKPAHTDYYNDVDLITPNHKEACEMLNLNPEL
                     SKDESSIEMIGSELKKRLDCNILLTRGEQGMALFLKSGEVIKIPTKAKEVYDVSGAGD
                     TVVASFALALAAGADYKQAAHIANYAAGIVVGKLGTATTTVSELKKAIEDEQSDFL"
     gene            complement(6508..7404)
                     /locus_tag="VFF28_01750"
     CDS             complement(6508..7404)
                     /locus_tag="VFF28_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012459750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZX44386.1"
                     /translation="MRCLVTGGAGFIGSNLALELEKQGHEVIIADSLFTGSKKNICRF
                     KGRLLDIDVSKPFEVDDKFDAIFHEAAITDSRFGDEAETIRANVDGFKNILKLVKKKD
                     AGLVYASTANLYGNGPVPMKENQEKQLISAYGKSKLLMDELAMKQGIGMHIVGLRYFN
                     VFGPNETNKGRAASMIYHLRNQIKEGKRPRIFKYGEQKRDHIYVKDVVEATIKALSAR
                     SGIYNVGTGIATSFNELVDCLNNAMGTSYKPEYFDMPYDHATYQENTQADTSLTEFCL
                     KFKASYSLLEGIKDYLRILDEG"
     gene            complement(7401..7766)
                     /locus_tag="VFF28_01755"
     CDS             complement(7401..7766)
                     /locus_tag="VFF28_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZX44387.1"
                     /translation="MTQHKEVNKVWGKEIWITNRDYCGKKLILKEGCQCSMHHHKNKD
                     ETFYILSGKVLMEAGKEKRIMLSGDSILIMPNTKHRFTGLKDSEIMEFSTHHEDSDSY
                     REEGMLSGKVDITKLELPR"
BASE COUNT         2124 a         1877 c         1420 g         2545 t
ORIGIN      
        1 gaagctaata gcccaggtct ttgccctgcc agcctgcctt attataccaa tgcattcttt
       61 tgtggtgagc tcttttcctt tacctttcat cttccagaca tcgcaaaaag agcaccttgc
      121 attgcaattg aatgtgacat cccaaccgat ggctagagga cctctgcttt tctttatgag
      181 tccaagatag agcatgatat tgtcgatgcc ggtgaatact cctttaataa atccctgccc
      241 ataaattttc ctaaactcat taagctcagc aattattgcc atttgacagc catacctctc
      301 cagctatttt tgtttctcca ggttatctta acatcagaca agcaaggctt gtcaaaccat
      361 ctttcaaact cctgctttgt ataatagaat gcgatcggag caaggagctg atcaaaaagg
      421 atagagtggt tctgctcgaa cttgaagtta cccagataat taaaaaatga attttggggc
      481 aagatctttt ctgcaatctt tcttgtcgat ttgctgctgt tcataggctt gtagatcagc
      541 tttgtcaaag gatgtatacc aagcatcgca atgaaagaca gcttgtaggt gagccctata
      601 ggaagcttag atatgacctg ctttcttata ggatccatca gcttcagcag ataatttcct
      661 tcctttccat aaacccagac agagatagtg cctccttttt ttatttttga gacaaggttg
      721 tcaaaagcct gctttggccc ggggaggtga tgaagaacac caatacaata cgcatagtca
      781 aacctgccag ccctgaacgg catattgtat atatctgcgt gtattatatg caccagagga
      841 aatttcaatg tatttttgta tgccacttca accgaatcgc taaaatcaaa cgctacagtc
      901 tctgctccat actttgccga atagtaagca tgcctgcctg tgccgcaacc tgcgtcaaga
      961 acaagctttc cagcaaaaaa atgctgattt actggataga tccaatccag gaactgattt
     1021 tcatacgatt cataaagctt tgagaaattt ttccattcgt agccaaatct ttccacagtg
     1081 ctttttttga tattcttatg atcatctttt atctttctat tactcccctt gagataatgt
     1141 ctgtatcttt ctgcaaaatc aacttctttt tccaggaagt cgttcatgaa atcatccgga
     1201 agcatcctgg gaatacctct aattacagga taaatcctcc tgcaggagca ttcaaggatg
     1261 gcttcagtaa tctcggtatt gaaacactcc ttgcaagctt cctttttctt aaaagtgcat
     1321 attttacaac gatttttttt acttacaaga taatgatttt tcttttcagc caatactttt
     1381 aggcttaatc tttttgaaca ataagggcat acaataaggt caagcaacct atacttcatt
     1441 tttttcacct gctgtattca tgagctgatc aacctgtcaa gatctaatat cggttttttt
     1501 ggatcattat ggatatatat cttgtaccaa acctggaagg caaacaagct ccacaactgc
     1561 cttgcataga acaatctgga tttatcaaag ttattaaata tcttctcaat cgcatcatag
     1621 gagaatgcgt tgctctccct aacactttgc cttgaaagcg cctggcccat caatcctttc
     1681 aagtcgcctt taaaccagct gtcaatcgga acaaaaaacc tctgcttttt cctctgcaat
     1741 atcagttttg gcagctttgc agagactgct tttcgcagta tatacttttc attgaatccc
     1801 tttagcttca agctttgagg gattgttgcc gcaaactcga caaaattatg gtcaaggaaa
     1861 ggaactcttg cctcaatgcc gaatgccata gacatcttgt ctgtcttcat gagcaagttc
     1921 tctggaagaa tatggtctat ctcaaggctg gttaaggaat caagaaggga ttttccagtt
     1981 gtttgtgcaa aatatgaggc atcgagtgca cccaccaagt ccacgctctt tgttgcttcc
     2041 ttatactcat cgcttaaaat acactttttt tccgactcat tgaatatcga tacgaggcta
     2101 agatacattt ttgcataatc atcagttgtg ataaattccc tgaacctctt taggccttct
     2161 tctcccaggg cgcttgaata cttaaagatg cggttcaaaa attttgcagg caaggcagct
     2221 gcgattccat aggcgcctct gcgtatgaac ttagggataa ctcttgcata agtgttatgc
     2281 aatttcatca tcttgtactg ctcatagccg ccaaaattct catctcctcc ttcaccggtc
     2341 agaacaacca cacactttgg ccttgttttt gaagcaagaa gatatgtggg tatgactgtg
     2401 ggatctgaca taggctcatc cagatgccag attatctttg gaaacaacct tgatgcatca
     2461 ggcttgacca ttatctcaca gtggtctgtc ccaaattttt ctgcgactat ccttgcatac
     2521 tttaactcgt tatcatatcc ttcgatgtcg aaaccgactg aaagagtctt tacaggctca
     2581 tcacttagtt ctgacatgat cgcaactatg gctgaggagt caatgcctcc tgaaagatag
     2641 gctccgagag gaacctcgct catcaacctg taatttatac tctttttcaa ggccgccaaa
     2701 agattcttgc tgatgacgtt ctcatcaacc ttcccatggc ggttatagtc cagactccaa
     2761 tatcttgttt tttttaactt gttatcttca tatataatat aatgggcagg aggcagtttc
     2821 atgatgcctt caaacatcgt ctcttctgtc gaaacacagc ggaaagtcag gaaatcgtgg
     2881 agggcttttg gatttacctt cctcttgatc tgcggatttt gaactatcgc cttaatctca
     2941 gaggcaaaca ccaaggcatc ccctaccagc gcgtagtaca ggggcctaat gcccagtctg
     3001 tcacgtgcaa gaaaaagtct tttttttgct gaatcccaaa tcgccataga aaaacagcca
     3061 ttgaattttt ttaggcattc agggccatat tcttcataag cgtgtaatat tacctctgtg
     3121 tcagagttgg tatagaacct gtgcttaccc agattatcgc gtatctcctt gaaattgtat
     3181 atttcaccgt taaatactat ccaaatgctc tcatcctcat tatgcatggg ctgcttgccg
     3241 ctttcgatat caataatgct cagacgccga tggcctagag aacaatgctt atctataaaa
     3301 aatccttctg catcagggcc tctgtgggaa atactcctta ccatgtcctt tagaaatctt
     3361 ccatcttcca taccagcaaa gccgcagata ccgcacatag ttacccctat ccaagaacct
     3421 tctctataac atagccctta tcctttgcgt ccttataata tgtccttatc agtatctctc
     3481 cgagaaagcc tagcacaata aattggacac ctagtattat caagatgcct gcaaacagga
     3541 ggacagggcc gattcccaaa gcaagggcgc cgtatcttat cgaatgatat gaaagatttg
     3601 caaaagccag cattactcca gccacaatct gaaaaaatcc aagtaggcca aagaaatgca
     3661 gaggccttgt cgaaaagctt gaccaaaatt tgatgtatac aagatcaaga agccccttta
     3721 gcagccgtgc gatgccatat tttgtctttc catggagcct gggttgatga ttgaccttaa
     3781 gttctccaac cttaaaaccg tttaatacaa ggatggcagg aataaagcga tgcatctcac
     3841 caaaaagagc caaccctttg atggattctt tggtgtaaac cttaaggctg catcctgagt
     3901 catggacata atcttttatc accaatctcc gaatatattc tgcaatcttt gagaagaacc
     3961 tttttgagaa gttatccttt cgtttgctgc gccagcctga aaccgcatca taaccttgtt
     4021 tttgtctttc aagcagacgg ggaatatcct tgggatcgtt ttgcaggtct gcgtccatgg
     4081 tgactatcaa tttacccttt gaggctgaaa atcctgcctg caaggcagct gtctggccga
     4141 agttgcgcct gaacctgata accttcacag atggatcctt attatgtaat ccttcaagga
     4201 cagagaaagt tgaatcagag ctgccatcgt caataaatat tatctcgctt tctttctgca
     4261 aactgtccag aacacccttc agctcctgat acacctgagg gatgctgtct gcttcgttat
     4321 aagcagggat gactattgac agctcaacct catggaccat tttaatgctt ggagaaaccc
     4381 cttaggattt ataaaagtgt cgatagacta agacaaaggc agcagcacag gtataccttt
     4441 tcttatggga taggtagcag cgcacttaac acagacaagc gttgtcttat ctttgccata
     4501 tttcaaggaa gccttgcagg aaggacaagc cagtaccttg aatagattat cttgtatcat
     4561 catttatcaa cctagtggtt gaaaaaccct gaatacggcc aactatataa atcttaccgc
     4621 cgtgtttttt tactgtcgga gcctctatgc attccttgcc gtattctgag ccattcacat
     4681 ggacatcagg gcgtatctcc tccaggagtt ctattggagt cttctcatca aatagaacta
     4741 cataatcaac aaagggaaaa tttgcaagca tcatggctct tgctttctca ccatttatcg
     4801 gcctgcctgg acctttattc tgcttaacgg acgaatctgt gttgaggcca actatcaaca
     4861 tatctccttg tttttttgcg ctttttaata taaattcatg gcctttatgc aagatatcaa
     4921 aagtaccgtt gatagtgaca attgtcttgc cttcttttct ttgctcatcc gcaatccttg
     4981 ttatctctcc ccttgagact attttcctgc gcctgttaaa catgataaaa tccgttgctt
     5041 ggccaaggcc tgctgctaca taatcaggct tatacttcct tacttctgcc agctccttgg
     5101 tgccatgtcc tgtcagcagg tgaattgttt tacagcttgc attctttcca agtttgatat
     5161 cagaagcatg atcgccaatg acccatgaat gcttcaggtt taggttattt ttcgcttttg
     5221 catccatgat gaactttgtt tttggctttc tacaatcaca tttatcttca gggctgtgag
     5281 ggcaataata tactttgctg aaatgcaggc cgtgcttttt aagctcgaag agaagatgat
     5341 tgttgaatct atggaaatcc tcatcggtat aaagccctct tcctatgcct gattggttgc
     5401 tgacaataaa gaagagaaaa cccttgaccc ttttaagcgc atcaacagca cccggaaaga
     5461 ggcaaaaatc ctggagctta tggacatatc ctggatcctt atttaagaca ccatccctat
     5521 caaagaaaat cgctttgttc atcttctatc gctttcttaa gctcagaaac cgtcgttgta
     5581 gctgtaccta gctttcccac cacaatacct gctgcatagt ttgctatatg tgctgcctgc
     5641 ttgtaatcag cacctgcagc aagggccaag gcaaaggatg ctacaacagt atctcctgct
     5701 cctgagacat catatacctc ttttgccttt gtaggtatct ttatcacttc accgcttttt
     5761 aaaaacaatg ccatgccctg ctctcctctt gtaagaagta tgttgcagtc aagcctcttt
     5821 ttgagctcac ttccaatcat ctcaatcgat gattcatcct tagaaagctc gggatttagg
     5881 ttgagcatct cgcatgcctc cttgtgattc ggagtgataa ggtcaacatc attatagtag
     5941 tcagtgtggg ctggttttgg atctattatc aaaaacttac catgcgcttt tatcaatgaa
     6001 cttgcctctt ttatcagatc ctttgttacg atgcccttga aatagtcaga gattacaact
     6061 ccatcaaacc catcgatact cttcttcaga aaatggatca tttttccttc agcctctttt
     6121 gagacaagga ccccgtcctc ataatcaaca cgcaagagct gctggttctg ggccattatg
     6181 cgcacttttt gaatagtttg cctatttttc tcagacatta tcccatctat cataatccct
     6241 ttcttcttta actcatctat caaacgatcc cttgcatcat cttcaccggt gacgcctatt
     6301 attgagacag tacccttaag tgaagcaaca ttatttgctg tatttgctgc tcctcccaaa
     6361 acaacacttt gcttttcaac atgaacgacc tggacaggag cttcagggct tattcttgta
     6421 acattacccc atatatactt gtctagcatt atatctccta tcaccagtat cctttttcct
     6481 tcaaactttt ccgttatctt aattagttca tccctcatca agtatcctca gatagtcttt
     6541 tatcccttca agcaggctgt atgaagcttt aaatttgagg cagaactctg tgagggacgt
     6601 gtcagcttgt gtgttttcct gataagtggc atgatcatag ggcatatcaa aatattccgg
     6661 cttatacgat gttcccatcg cattgttcaa gcaatcgacc agctcattga agctggtggc
     6721 gatacctgta cctacattat aaatgccgct cctggccgaa agcgccttta ttgttgcctc
     6781 gacgacatcc tttacatata tatggtccct tttttgctct ccatacttaa atatcctggg
     6841 cctttttccc tcttttatct gattcctaag atgatatatc atggaagctg ccctgccttt
     6901 gttagtctca ttcgggccaa aaacattgaa atagcgtaga ccaacaatat gcattccaat
     6961 cccctgcttc atggccaact catccatcag aagcttagac ttgccatatg ctgatatcag
     7021 ctgtttttcc tggttttcct tcattggaac aggtccatta ccatatagat ttgccgtcga
     7081 tgcgtagact aatcctgcat ctttcttttt taccagcttt aatatgtttt tgaacccatc
     7141 aacatttgcc cttatggtct cagcttcatc accaaacctc gagtctgtta tcgctgcttc
     7201 atgaaagata gcatcaaact tatcatccac ttcaaacggc tttgaaacat caatatctaa
     7261 caggcgacct ttaaagcggc agatgttttt cttagaacct gtaaacaagc tatctgctat
     7321 gataacctcg tggccttgtt tttcaagctc tagagctagg tttgagccaa taaatcctgc
     7381 tcctcctgta acaaggcatc tcatcttggc agctccaact ttgtgatgtc gactttacct
     7441 gacagcatgc cctcctccct ataagaatct gaatcttcgt gatgggtgga aaactccata
     7501 atctctgagt cttttagacc ggtaaagcga tgctttgtgt ttggcattat aaggatggaa
     7561 tcgccagaaa gcattatcct cttttctttt cctgcttcca tgaggacttt tccgcttaag
     7621 atatagaaag tttcgtcctt atttttatga tgatgcatgg agcattgaca gccttctttt
     7681 aggataagct tttttccgca atagtctcga tttgtaatcc atatctcctt tccccacact
     7741 ttgttgactt ctttgtgttg tgtcatttgg tattaccttt tcataccttt gaatcttaat
     7801 cactatataa atctttctct atattcggct ttgcgcacaa attatccctg aatcctgtta
     7861 aaatatgata gtcttgccct gttatgcaag attatccgag aaatcaattg aacaaactta
     7921 caatggcccc ttaccttgct gattatacca aagacccttt tgattg
//
LOCUS       DASRUK010000039         8406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_700375, whole genome shotgun sequence.
ACCESSION   DASRUK010000039 DASRUK010000000
VERSION     DASRUK010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 8406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8406
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700375"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VFF28_01760"
     CDS             <1..181
                     /locus_tag="VFF28_01760"
                     /EC_number="2.7.4.14"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of (d)CDP from ATP and
                     (d)CMP; Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cytidylate kinase"
                     /protein_id="HZX44388.1"
                     /translation="QDIKESQEKIKQRKRSEDKRYMDYYKVNYQKESNYNLVIDTTNL
                     SPEKVIKAIRKSISL"
     gene            276..959
                     /locus_tag="VFF28_01765"
     CDS             276..959
                     /locus_tag="VFF28_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZX44389.1"
                     /translation="MINKKDFIELEYTGKIKGTDEVFDTTSEKVAKDSGIYSKGAQFK
                     PVTIIVGEGQLVKGLDNDIIGKEPNKEYDLDLKPEDAYGSKNAKYIQLISTAKFLKQQ
                     IQPMPGLQVNIDGRVGTIKTVSGGRTLVDFNHPLAGKELSYMYKIIKVITDEKEKIAS
                     VLKANLDIDSTVTLEGKKAKIIIKEELPEEIKAELEKKIKEIIPMDSIAIEKEENLNN
                     STPKKEIGG"
     gene            960..2075
                     /gene="ftsZ"
                     /locus_tag="VFF28_01770"
     CDS             960..2075
                     /gene="ftsZ"
                     /locus_tag="VFF28_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HZX44390.1"
                     /translation="MDKFITEAINVASRDYSNENRQKNAVDAELEAFLAQQKATIKVV
                     GCGGGGNNTINRITEVGVKGAESIAINTDAQDLLYTTADKKILIGREITKGMGAGANP
                     KLGEEAAREQEQDIKKALEPADMVFITCGLGGGTGTGSAPVVAEVARKMGALTVAIVT
                     MPFAMEGHKRYENAVTGLEKLEQIVDTLIVIPNDKLLELAPDLPLHTAFKVADEILTN
                     SVKGIAELVTKAGLVNLDFADIRAVMKGGGVALIGVGESDTENRAIEAVEKAINNPLL
                     DVDITGANGALINVAGGEDMTLDEARKVVETVSEKLNEDAKIIWGAQIYKDLKNTIRT
                     MLIVTGVKSSQIFGGDVTTTGKKKKEIEKELGIDFLD"
     gene            2077..2256
                     /locus_tag="VFF28_01775"
     CDS             2077..2256
                     /locus_tag="VFF28_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011406844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase SEC61 complex subunit gamma"
                     /protein_id="HZX44391.1"
                     /translation="MDNIWLRFKGFVNESIRVLRITKKPDKTEFMTIVKASGLGILVI
                     GLIGFVIQMVKVLFF"
     gene            2256..2750
                     /locus_tag="VFF28_01780"
     CDS             2256..2750
                     /locus_tag="VFF28_01780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor Spt5"
                     /protein_id="HZX44392.1"
                     /translation="MEAEKNDRENEVIYPLRVTANREDQVMDFVTANAKNKKLEVYSV
                     IRPHGMRGYIFIEAATRTDAEQAAFNVPYARGILPKTVEYKEIEHMLEQVKKEVNIQK
                     SDIVEIISGPFKRENAKITRIDKQKEEVVVELLQAAVPIPITLRLDSVKVIRRESDEE
                     EEKD"
     gene            complement(2911..6966)
                     /locus_tag="VFF28_01785"
     CDS             complement(2911..6966)
                     /locus_tag="VFF28_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44393.1"
                     /translation="MKKLIWAFVLVVMLGVVEAKVDVIKVSPQAVEPGQTLSVTVKGE
                     EYYSYGDAFDSAGSIGKVPIRCGATTRCSGESSFRFIVPATAKLGRARIELTDYKAAK
                     GFEKFNVNFDIIAKAKTFECYSQFNTKAHSGKCDPKNCGEASMVKCTNLWVVNFETCS
                     RTYTTECSKTPSCKAGYSVKSAKDCSLQTCSDSDNGKDYFTKGKASGKKLGADYVETY
                     EDVCLTDDEAGRDLKEGYCSDNQATFEFTKCPNGCKDGACIKQVECVDSDSGKNYESK
                     GKITSSNSQGEDKCANANTLNEMFCSGNLGAMEIHKCENGCKDGACVKDNITKCTDSD
                     GGKNYNVIGLTNKAKLSASSEAVIWKDICLKDFDKYQKGEYLPQQGVTKPNGLLEGSC
                     GINSNEQGTISAYECPNGCKDGACVKENASKCTDSDGGKNIFEAGKTKRRDGNFWTDT
                     CVNPSQVQEGYCDDRSTTHVSTYVASCANGCKDGACVKGNPNNCTDTDGVEIYTKGIL
                     SWTTVEGRRQTMTDQCSGYNNESVVDYVCGKTAGSYYETVETKCPNGCKDGGCLQKNE
                     SPCTDSDGGKNYYTAGKTTSDWTNLGVGSKQTKADSCSSNGQLTEYSCSTTPPYVGSI
                     SATTITCPNGCKDGSCINSSCGNGIIDKEEQCDSGSLNGKDFCSDKCVFLGKRELAVV
                     CQLMTGVIQTDRSCSVSFGALTGSCILKKGQNQCITHLLLPANANELNYKSHLKGSIE
                     GGSMKNNAYNGVNYFVNFDLNETPCVDTEGGKDYYNKGETTGYTQGVNQVYTSKDMCL
                     MDGQNNADLQETWCENNKVHVERYNCQNGCKDGSCIKESTKICTDSDGGINYNTAGTV
                     TGIERRADGSLVVFPDGSFDYGDFKDQCYQDALVEFYCKEDGGVSAEQYQCLNDCKEG
                     ACIQGGARLVILQDFGTIKTSNEFESGPNADMVDYMAKIFSGFKDGESTRYKLEQTQY
                     NPDIGVAVAEFEKEISFVDFDRDFVKKLKASNTNLMLDYDSANVEGSLPEAQILFYSD
                     NNRDQGVIWLSKNIVVFAYVGDIKSIGQKPQGEDWIDFLTAYVKRFPSTLVPCEGDNC
                     EANLVCKDSDGGNLYEKGYSSLAENHEDTCRGNSVIEDFCSDSNIIDKNDYFYLAGAG
                     KDVLLRYKGSDRCDKGVAWLFGGAAKFDVIDTGKRITFPLQKEGYFTLNYNGIKTKMK
                     LTDCMEDSSIDTLGTPLLREKRVECSNGCSDGACKEDNRQVTYEGVLQMLNKCQAFEM
                     GSAEPKKHFGKDYASGNDMCNWRANQDGTDLTCITAGRIYSIDSRQTGYGTWSCERDV
                     VGPSHNGWALCCKVPGPISPPAPSIPEPVAE"
     gene            complement(6968..7702)
                     /locus_tag="VFF28_01790"
     CDS             complement(6968..7702)
                     /locus_tag="VFF28_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44394.1"
                     /translation="MKKGSLSLSINAIVILIISIAVLLLGIGFLSGFFGKTDENLKEL
                     IGKEQDAPSPSASNSMTLSRERIVTSPGSTEIVKVAVMNPSKKQWVGRKDLYFNCAGC
                     NQGDGVCFAGGGTKCKAVPYSDACYGTDFDCLGDDIAGDSCVSGDGVCYVGPGCDEGN
                     EIEDADCGPRTEGIDLVISCGGSLVGGQYSNSKKVKPGEIAEFVSLIKVSEDASSAIC
                     SYRIYGYDEEGNNVDGIKKELTIEVQ"
     gene            7921..>8406
                     /locus_tag="VFF28_01795"
     CDS             7921..>8406
                     /locus_tag="VFF28_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010867125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HZX44395.1"
                     /translation="MAKETVEVMVEGGKATAAPPLGPALGPMGVNIGQVVGKINEKTK
                     SFAGMKVPVKVIIDKSTKQFEVEVGTPPCSQLLKKEANIEKGSSNPLEDKVADVLIEQ
                     IIKVAKMKEGALLGKTLKSKVKEVIGTCNSMGILVEGKSAKEATAEVNAGKYDAEIKA
                     EK"
BASE COUNT         2488 a         1865 c         1693 g         2360 t
ORIGIN      
        1 ccaagacata aaagaatctc aggaaaagat aaagcaaagg aagcgctcag aagataaaag
       61 atacatggac tattataaag tcaactatca gaaggaatca aactacaatc tggtcataga
      121 cacaacaaat ctctcgccag agaaggtcat aaaggccatc agaaaatcga tatctttata
      181 aatcgataac cactcacaaa acagattagt tctctaaaaa gctatcaaga gctaaagaga
      241 aaacctttta aaaaacaaga gctaatccca aaaaaatgat aaacaaaaaa gattttattg
      301 aacttgagta tacaggaaaa attaagggca ctgacgaagt ttttgataca acaagcgaga
      361 aggttgcaaa agacagcggc atctacagta aaggagccca gtttaagcca gtcactataa
      421 tcgttggcga aggccagctt gtaaaaggcc ttgataatga tattatcgga aaagagccaa
      481 acaaggaata tgatcttgac ctaaagccag aagatgccta tggcagcaaa aacgccaaat
      541 atatacagct tatatcaact gcaaagttcc taaaacagca gatacagccg atgccgggcc
      601 ttcaggtaaa tatagatggt agggtgggaa cgataaagac agtctcaggc ggaaggacac
      661 tggttgactt taatcatccc ttggcaggaa aagagctgtc ttatatgtac aagattataa
      721 aggttataac cgatgaaaag gaaaagattg cctctgttct caaagcaaac ctggacattg
      781 actcgacagt caccctggaa ggcaaaaaag caaagataat cataaaagaa gagcttccag
      841 aggaaattaa ggcagagctg gaaaagaaaa taaaagaaat catacccatg gacagtatag
      901 ccatcgaaaa agaagaaaat ttaaataata gcacccctaa gaaggagata ggtgggtaga
      961 tggataagtt cattactgaa gcgataaatg tagctagcag ggactattct aatgaaaata
     1021 ggcagaaaaa tgccgttgat gcagagcttg aagccttcct tgcacagcag aaagcgacta
     1081 taaaggttgt cggctgcggt ggcggtggaa ataacaccat caataggata actgaggttg
     1141 gagtaaaagg cgctgaatct atagctatca acacagacgc tcaagatctt ctttatacaa
     1201 cagcagataa aaagatactg attggaagag agataacaaa aggcatgggt gcaggagcaa
     1261 acccaaaact aggcgaagag gccgcaagag agcaggaaca agatataaag aaggctttag
     1321 agccggctga catggtattt atcacatgcg gacttggcgg cggcacagga acaggctctg
     1381 ctccagttgt tgcagaagtc gcaagaaaga tgggcgccct tacagttgcc atcgtcacta
     1441 tgccttttgc catggaaggc cacaaaagat atgaaaacgc agtcacaggc cttgaaaaac
     1501 ttgagcagat tgttgataca ttgatagtca ttccaaacga taaactgttg gagcttgcac
     1561 cagacttgcc gcttcacaca gcatttaagg tagcagacga aattcttaca aactcagtaa
     1621 aaggcatagc agagcttgtc acaaaagcag gccttgtcaa ccttgatttt gctgatatta
     1681 gagcagtcat gaaaggcggc ggtgtcgcat taataggtgt aggcgaatca gacactgaaa
     1741 acagggcaat agaagcagtt gaaaaagcca tcaacaaccc attattagac gtagatataa
     1801 caggagcaaa cggggctttg ataaacgttg caggcggcga agacatgacc ctcgatgagg
     1861 caagaaaggt cgttgagact gtctcagaaa agcttaacga ggatgcgaag attatctggg
     1921 gagcgcagat ttataaagac ctaaaaaata ccatcaggac gatgcttata gttacaggag
     1981 tcaagtcatc acagatattt ggcggagatg taaccaccac aggaaaaaag aaaaaggaga
     2041 ttgaaaagga gttaggtata gacttcttgg attgatatgg ataatatttg gttgagattc
     2101 aaaggatttg taaacgagag catcagggta ctgaggatta caaaaaagcc tgacaagaca
     2161 gagtttatga ccatcgtcaa agcatcaggc ctgggtattc tagtcatagg tcttattgga
     2221 tttgtgatac aaatggtaaa ggtgttattc ttttaatgga agctgaaaaa aacgataggg
     2281 aaaacgaagt gatttatccg ctgagggtaa ccgctaaccg tgaagatcaa gttatggatt
     2341 ttgttacagc aaacgcaaag aacaagaagc ttgaggtcta ttcagtgata aggcctcatg
     2401 gcatgagagg ctacattttc atagaagcag ccacaaggac agatgccgag caggctgcat
     2461 ttaacgttcc gtacgcaaga ggtattcttc caaagaccgt tgaatacaag gagattgagc
     2521 atatgcttga gcaggtcaaa aaggaagtaa acatccaaaa gagcgatatc gtcgagataa
     2581 tttctggccc cttcaagagg gagaacgcaa agattacaag gatagacaag caaaaggaag
     2641 aagtcgttgt cgaactcctt caggcagctg ttcctatccc gatcactttg cggttggatt
     2701 ctgtcaaagt tatcagaaga gaaagcgacg aagaggaaga aaaggattaa ttccttatct
     2761 atttttttct tgttttgtct gagacgagac taaaatcctg ggactttagt ccagtggaca
     2821 atagttatct ctgaggatcg atccgtctaa ctaaataaca accatgcatt ttagtgcata
     2881 gttagggtaa tatcgacgga tattcaaatc ttactccgcg acaggctcag gaatagacgg
     2941 agctggcgga gatattggcc caggaacctt gcagcaaagc gcccatccgt tatggctagg
     3001 tcctacaaca tctcgttcgc aagaccatgt gccatatcct gtctgcctgc tgtctataga
     3061 gtatatcctt ccggctgtta tgcatgttaa atctgttcca tcttggtttg cccgccagtt
     3121 gcacatgtca ttacctgatg cataatcctt tccaaagtgt ttctttggct cagcgcttcc
     3181 catctcaaat gcctggcatt tgttaagcat ctgaaggacg ccttcatatg ttacctgtcg
     3241 gttgtcctct ttgcaggccc catcagaaca tccgtttgag cactcaactc tcttttctct
     3301 aaggagaggg gtgccaagcg tatctataga tgagtcttcc atgcaatctg ttaatttcat
     3361 cttagttttg attccattat agttcaaggt gaaataacct tctttttgca gggggaaggt
     3421 tatcctcttg cctgtgtcta taacatcaaa tttggcagca cctccaaaaa gccaagcgac
     3481 gcctttatcg cacctatccg atcctttata cctaagcagc acatccttcc cagcgccagc
     3541 aagatagaaa taatcgttct tgtctattat gttgctgtcg gagcagaaat cctcgattac
     3601 actattccct ctgcaggtat cctcgtgatt ctccgcaagt gaactgtatc ccttttcata
     3661 caaattaccg ccatcagagt ctttacatac aaggttagct tcacaattat ccccttcaca
     3721 aggtactaat gtagaaggga atctcttaac atacgctgta aggaaatcga tccagtcctc
     3781 tccttgaggt ttttggccta tggattttat atctccaaca taagcaaaga ctactatatt
     3841 tttgcttaac cagataactc cttgatcccg gttattatca gaatagaaca agatctgggc
     3901 ttctggcaga cttccctcaa cattggcaga gtcatagtct agcatcaagt tggtatttga
     3961 agcctttagc tttttcacaa agtctctgtc aaaatccaca aaagagatct ctttctcaaa
     4021 ctcagctact gcaactccta tgtccggatt atattgtgtc tgctcgagtt tataccgtgt
     4081 ggattcacca tccttaaatc cagaaaatat ttttgccatg taatcgacca tatctgcatt
     4141 tggccctgac tcgaattcat ttgaagtctt tattgtacca aagtcctgaa ggattaccag
     4201 tcttgcacct ccttggatgc aagccccttc tttgcagtcg ttcaaacatt gatactgctc
     4261 tgctgagacg ccaccgtctt ctttgcaata gaattctacc aaggcatctt ggtagcattg
     4321 atccttaaag tcgccataat caaaggagcc atcgggaaat actactaagc tgccatctgc
     4381 cctcctttcg atacccgtga cagtgcctgc agtattgtag tttattccgc catcggaatc
     4441 tgtgcatatc tttgtgctct ctttgatgca agaaccatcc ttgcagccgt tttggcaatt
     4501 gtatctttcc acatggacct tattgttttc acaccatgtt tcctgtaggt cagcattatt
     4561 ttgcccatcc atcaaacaca tgtccttgga tgtatatact tggtttactc cctgcgtata
     4621 acctgtagtc tctcctttat tataataatc cttacccccc tcagtgtcaa cacaaggtgt
     4681 ttcattaagg tcaaagttaa caaaataatt tacgccatta tacgcgttat tcttcatcga
     4741 gcccccttcg atactgccct ttagatgaga cttataatta agctcatttg cattagcagg
     4801 taatagaagg tgggttatgc attggttttg gcctttcttt aagatgcagc tgccagttaa
     4861 ggctccaaag gatacactgc atgagcgatc agtctgaata actccagtca ttaattggca
     4921 aacgacagct agttctcttt tacctaagaa cacacacttg tcagagcaaa aatcctttcc
     4981 attgagcgat ccagaatcgc attgttcttc tttgtcaatt atgccatttc cgcatgaact
     5041 atttatacaa cttccatcct tgcagccgtt tgggcatgtt atagtcgtgg ctgatattga
     5101 tcctacatac ggaggcgttg ttgagcaaga gtattctgtc agctgcccat tactgctgca
     5161 tgagtcggcc ttggtctgct tgcttcctac tccaaggtta gtccaatcag aagttgtctt
     5221 gccagcagtg taatagttct tgccgccatc agagtctgtg catggactct cgttcttttg
     5281 gagacaacct ccatccttgc agccgtttgg acatttagtt tccactgttt cataatatga
     5341 ccctgctgtt tttccgcaaa cataatccac cacagactca ttgttgtatc cagagcattg
     5401 atcagtcatg gtctgccgtc ttccctcaac agttgtccag cttagtatac ctttagtata
     5461 tatttcaact ccgtctgtgt ccgtgcaatt atttgggttt ccttttacgc aagcaccatc
     5521 cttgcagccg ttcgcacagc tggctacata agtgctaaca tgggtggttg aacgatcatc
     5581 gcaatatccc tcttgtacct gcgaagggtt cacacaggta tcggtccaga aatttccatc
     5641 tctccttttg gtttttccag cttcaaagat atttttacct ccatcgctat cagtgcattt
     5701 cgaagcattt tccttaacac aagcaccatc cttgcagccg ttggggcatt cataagcaga
     5761 tattgttccc tgctcattag aatttatgcc gcaagatccc tcaagtaatc cgttaggttt
     5821 agttacacct tgttgaggca gatattctcc tttttgatat ttatcaaagt ctttaaggca
     5881 aatgtctttc caaataactg cctcgctact tgcgctcaat tttgctttgt tcgtgaggcc
     5941 gataacatta taatttttcc cgccatcaga atccgtgcat tttgtaatat tatccttgac
     6001 gcaagcgccg tctttgcagc cgttctcgca cttatgtatc tccattgctc caagatttcc
     6061 gctgcaaaac atctcgttca aagtgtttgc gtttgcgcat ttgtcctcgc cttggctgtt
     6121 gcttgatgtg atctttcctt ttgattcata gttctttcca gaatcgctat ccacacattc
     6181 cacttgtttg atgcaagctc catctttgca gccgtttggg catttggtaa attcaaaggt
     6241 agcctggttg tctgaacaat aaccttcctt gaggtccctt cccgcctcat cgtctgtcag
     6301 gcatacatct tcataggtct caacataatc cgctccaagt tttttgcctg aagctttccc
     6361 ctttgtaaaa taatcctttc cattatcaga atcgctgcaa gtctgcaatg aacaatcctt
     6421 tgctgatttg acgctgtatc ctgccttgca gctaggtgtt ttgctgcatt cagtagtata
     6481 tgtgcgggag caagtttcaa aatttacaac ccagagattg gtgcatttta ccatgcttgc
     6541 ttcaccacaa ttttttggat cacacttgcc tgagtgggct tttgtgttga actggctgta
     6601 gcactcgaag gtttttgctt ttgcgatgat atcaaaattg acattgaatt tttcaaagcc
     6661 ctttgcagcc ttataatctg taagttctat ccttgctctc cctagctttg cagtggcagg
     6721 cacgataaat ctgaatgatg attccccact gcatcgtgtt gtggctccgc aacgtatagg
     6781 caccttaccg atgcttcctg ccgaatcaaa ggcatcccca tacgaataat attcttctcc
     6841 cttgaccgtg accgataaag tttgccctgg ttcaactgcc tgaggagata ccttgatgac
     6901 atcaacttta gcttcaacga cccctaacat aacgaccaat acaaaagccc agattaattt
     6961 tttcatttta ttgcacctct atggttaact ctttttttat tccatcaaca ttattacctt
     7021 cttcatcata tccatatatc ctgtatgagc atatagcgct tgaggcatct tcacttactt
     7081 ttattagtga gacaaactcg gcaatctctc cgggttttac tttcttggag tttgaatact
     7141 gcccccctac caggcttcct ccacagctga taacaagatc aattccttct gtccttgggc
     7201 cgcaatcagc atcttcaatt tcattgcctt catcgcaacc cggcccaaca taacagactc
     7261 catcaccgct tacacagctg tctcctgcaa tatcatcgcc aagacaatca aaatctgttc
     7321 cgtaacaggc gtcgctgtaa ggaactgcct tgcactttgt tcctccacct gcaaagcata
     7381 caccatctcc ctgattgcac ccggcgcagt taaaataaag atctttccgt cccacccatt
     7441 gttttttgct cggattcatc acagcaacct tcactatttc tgtgctgcca gggctggtca
     7501 caattctttc tctcgaaaga gtcattgaat tgcttgcaga agggctcgga gcatcctgct
     7561 cttttcctat cagctcttta agattttcat cagtttttcc aaaaaatcca gaaagaaacc
     7621 ctatcccaag aagtaacacg gcgattgaaa tgattagaat gactatagca tttatcgaca
     7681 gcgataagct cccttttttc atcttgactc ttcagtatac ccttgtctat actgatatgg
     7741 gttgtatata aatatatcga caactccatg ataaaatggt taattcaaac aagcaccaaa
     7801 tagtgtcact gtaaatataa atctattatc cagaatgggt aattacctag actgctgtaa
     7861 caagaaagaa ccgattcgta aatggaaagc tatttaaata aaataccaat tccacacaat
     7921 atggcaaaag aaaccgtcga ggtcatggtt gaaggtggaa aagctacagc agctccgcct
     7981 ctaggcccgg ctttaggccc gatgggtgta aatattggcc aggttgtcgg caagataaac
     8041 gagaagacca agtcttttgc cggaatgaaa gtacccgtaa aagtgatcat agataaatcg
     8101 accaagcagt ttgaagtcga ggtcggaacc cctccttgtt ctcagcttct aaaaaaagag
     8161 gcaaacatcg aaaaaggctc gtctaatcct ttagaggata aagtcgcaga cgttcttatc
     8221 gagcagataa tcaaagtggc caagatgaaa gaaggtgcct tgcttggcaa gacattaaaa
     8281 agcaaggtaa aagaagttat aggtacatgc aattctatgg gtattcttgt cgaaggaaag
     8341 tctgcaaaag aagcaactgc agaagtaaac gcaggaaaat atgatgcaga gataaaagca
     8401 gaaaag
//
LOCUS       DASRUK010000040        10759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_700492, whole genome shotgun sequence.
ACCESSION   DASRUK010000040 DASRUK010000000
VERSION     DASRUK010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 10759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10759
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700492"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..1464)
                     /locus_tag="VFF28_01800"
     CDS             complement(223..1464)
                     /locus_tag="VFF28_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44396.1"
                     /translation="MAFVFQLRGVSKTESKEICKRYTKEFKRKDNFHTAVCGLFLDII
                     YLYFDNDKTKLYKLFFPVIMANFTEKGSIHSVIEEYRESKNLYSWSYTYRILNMVDKD
                     DGLEIGRIFRKLLLKLMKNAAFKNKGYCVAADITAKPFYGDKNLLMVKGCKRKAGTNY
                     AIQYLTASIVEEGVRFNLLCLPIPSLASVVGKFEQMIREIRKLVPIKLLFLDRGFGNK
                     EYSRVLGLLCHKYIMPITRNHKLKEIELCMKEQSTIKDDEYCVSILDYTFSEDRPKEY
                     QLKVRLIIVQDKDDVFFFITNMYNLSMRQYYLLSETYKYRFGIETNYRVDNIFSPFTC
                     STKAALRYLLMQMSLIAQDLRAFVNFISHKKGKRQPRERFKKGRSVMDIVRARIRDLG
                     YTWRPVITAVQFKRKIDKILG"
     gene            1577..1756
                     /locus_tag="VFF28_01805"
     CDS             1577..1756
                     /locus_tag="VFF28_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44397.1"
                     /translation="MFVRKKRFKRGDKYSTYYYLEKQEKVGGKFQTKTVRYLGTAEKI
                     LETYEELEKLKRKIV"
     gene            complement(1791..2033)
                     /locus_tag="VFF28_01810"
     CDS             complement(1791..2033)
                     /locus_tag="VFF28_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44398.1"
                     /translation="MRDCRPPLVSPETAIDKLADDLFRAAREAFVESQTKPTRSQFLL
                     QARQHLNGSTALECVDRIQIYYDAELAKMGYNGGKQ"
     gene            complement(2065..2595)
                     /locus_tag="VFF28_01815"
     CDS             complement(2065..2595)
                     /locus_tag="VFF28_01815"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HZX44399.1"
                     /translation="MKNINRRVILRRRNRLSATEIKEKSDKIKQILFNSKEYRQANIV
                     MFYVSFGSEVDTWDMIKESLEEKKVCVPCVIKDRLVASKIASPGELSKKNVYGICEPE
                     KIKEVSKDVIGLVIVPGVVFDKHNHRIGYGKGYYDDFLKEFSGKKIGLAFELQVLEDI
                     PRDDWDIRLDKVISEG"
     gene            complement(2634..4190)
                     /locus_tag="VFF28_01820"
     CDS             complement(2634..4190)
                     /locus_tag="VFF28_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44400.1"
                     /translation="MTPKTVIVFSLLLAVLSTTSTAKELTLAVNQTEYYFLVNENAII
                     PIEFDNPSSTPIQGTLSYTITQEISQAGFQYSSSNSGSQPFTAPGGKQIVTFSAGTSD
                     KPIKLTIDLTFAYGSVNINLRGIIVYFVQDQSQKQKNDERKESKQQNNQQQTSQQSNQ
                     QSQQSQMQQAKQQMQQLQQQMQEQMNKIFGSQQSKPDPMQAVQNNQMAQDSSALKQQL
                     QKQMQEQQQMKQEFQNNLGQNPEFQQKHNDLTQQGYKPMSGNLDPTNSTSGNFEVNYE
                     KGAETATVKGSMENNQMKEIQKTTSEDIKKIMDQLLNDQRYQQYSQYLQSQGYTPKQP
                     EVQTAGNATSIKIPYQDDNNKTAAITAQIKNNVVEDIKLEKEKSLWWIWALSLPLLAI
                     GIYFAYTKYLRKSADQMPHAVIERPIDYRKESRNMLDEAKKLYREKKYKDAYEKASQA
                     VRFFYSHKLGIRIELTNSELIKHLKKEKISHEDTQKCLNLCSMVEFAKYQANDEDFNQ
                     IISTSESMIK"
     gene            complement(4192..5142)
                     /locus_tag="VFF28_01825"
     CDS             complement(4192..5142)
                     /locus_tag="VFF28_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZX44401.1"
                     /translation="MAFSFQYPHLLWFLLIVPVIFFFHRLADQKKKKEAIKFSHLSII
                     KKAMGDKKPIRVKLLFYILLTAIALMLMGLADPHIPMKQTKEGVNVVLVIDDSGSMQA
                     TDYKPNRLEAAKNSAAILIESLKPNDHVGIVIFENGATTACYLTPFKDRALDKLKGIT
                     AREGKTAIGDGLSLGIDMATSIPNKKKVVVLLSDGVNNAGVISPQEAIQFAKSNKIQV
                     HAIAMGSEGNVVLGYDWFGNPQYAELDEETLKDIAQQTGGSYFKSVDEKTLSDIYKNI
                     SKDIKREKENTSIKDWFFGSAVMLLLVEVYLRYGRNRIIQ"
     gene            complement(5145..6026)
                     /locus_tag="VFF28_01830"
     CDS             complement(5145..6026)
                     /locus_tag="VFF28_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZX44402.1"
                     /translation="MPETIEILKKVKKIEIKTKHLVDGLLSGAYHSVFRGRGIEFSEV
                     REYVAGDDIRSIDWSVTARMNHPYVKEFIEERDLTIYIVLDVSASNDFGSEKSKKEAA
                     IELSASLMFAALRNNDKVGLCLFTDKAEKYYSPRKGKTHILRLVRDMIEYNPKSKATG
                     LAETLRFLSKVIKKRSIIFIISDFFTDSFEKELKILKNKHDVIAINMSDIRESEIPNV
                     GYIELEDEETGEQLLLDTSDKEFRQSYISMMKERNDRLNNMLKKLKIDNIQLKSQEPY
                     EIPLRKFFRLRERRMVR"
     gene            complement(6035..6808)
                     /locus_tag="VFF28_01835"
     CDS             complement(6035..6808)
                     /locus_tag="VFF28_01835"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HZX44403.1"
                     /translation="MSKVANAMLFFVLGFIVAVAIFGYPTAEKVTGQAIQEQDAQNTT
                     CIGPINQTIERSLAIVAVSEDEDEGIIGNLTVRLIPGNSNVLIETNPFLEPDLQYSAN
                     IAKEVAIEVTGKSNPDRDFVFTYSIESQVVGGGSAGAATTLAAIAALDNKTIRQDAII
                     TGTINSDGTIGRVGGILEKAKAASDAGYKLFLVPRGQTVVRYYEREVTREQTGLGFVF
                     YNTRYVPKVLNITESVKEWGLELREVSNIKQAADIMLRD"
     gene            complement(6805..7791)
                     /locus_tag="VFF28_01840"
     CDS             complement(6805..7791)
                     /locus_tag="VFF28_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZX44404.1"
                     /translation="MDKEIKLLNAQAEKYNKQIVKALEEIKKVIVGQEEVLEKLMISL
                     IADGHVLLEGVPGLAKTLMIKTLAETLNSRFSRIQFTPDLLPADIIGTKIYEHKTGTF
                     TTKKGPIFTNFILADEINRAPPKVQSALLEAMQERQVTIQGDTFKLEKPFLVLATQNP
                     IETEGTYKLPEAQVDRFMFKLLIKYPSKKEEMEIIRRNTQGREFDLAKVINPDDIIKI
                     QKFNQQIYADEKIEEYVSSIVDATRHPLEYHLDFDGHIEYGASPRASIWLILAAKAHA
                     LLHQRGYVMPEDVKAVAHDVLRHRIILSYEAEAEEITSDDIISKIIEKVKVP"
     gene            complement(8330..8596)
                     /locus_tag="VFF28_01845"
     CDS             complement(8330..8596)
                     /locus_tag="VFF28_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HZX44405.1"
                     /translation="MDVAFTRMSSKGQIVIPSEMRVGIDEGDKLLLIKNDSRIIIKKA
                     SELDENLKDDLIFAKRTEEALKRYESGDFKTVPASEFLKMIEKC"
     gene            complement(8650..9966)
                     /gene="purD"
                     /locus_tag="VFF28_01850"
     CDS             complement(8650..9966)
                     /gene="purD"
                     /locus_tag="VFF28_01850"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HZX44406.1"
                     /translation="MNVLLIGNGAREHAMAEAIIRSPKKPKLYSYMKANNPGIASLSY
                     QVKLGKYDDLSTITSYAKASKIDIAVIGPEDPLSDGVVDALEKEGIKAVGPKKQLAQL
                     ETSKSFTRNLMKKYNVPGAPIFGVFNSTKGLKEFLIDLHPKIVLKPDGLTAGKGVKVQ
                     GDHFTTVEEAYQYCEEVLKSHSAVVVEEKLEGEEFSLQSFTDGFSLLHCHPVQDHKRA
                     FAGDKGPNTGGMGSYSTGELLPFMEKSDLEQAKEITDKMREALKKECGEPYKGVMYGG
                     FICTKEGTKLIEYNARLGDPEAMNVLPIMNNDYVDVLQAIADGTLSKIKADFRNISTV
                     CKYAVPNGYPDHPVENQKVSLDNVPKNVKVYYASIDKREDGLYMSKSRALAFVGIAKD
                     IEEAQNIAEEGVKAAKGPIFHREDIGTKNLIDKRIKHMQRLRQKSN"
     gene            complement(9944..>10759)
                     /locus_tag="VFF28_01855"
     CDS             complement(9944..>10759)
                     /locus_tag="VFF28_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HZX44407.1"
                     /translation="ISRKGIEIHNQGNHESKVCAFLWIYTGFPASSYEGINVEQVREN
                     CGKLLAKSDDVGVDLVSGVPDSGTAHAIGYAIQSGKPYRRALIKYTSGYGRSYTPLTQ
                     DLRDRIALMKLIANEDVIKDNRILLCEDSIVRGTQLKNYTITKLKKAGAKEIHIRPAC
                     PPLLFPCIYALSTRTNHELVARRAIRAIEGKEIEDISEYLNPESEKYKKMVDWIAKDL
                     GVNSLKYLKLDDMISAIGVAKEKICTYCWTGKSIQMQLNRYQQEVYNECLTHR"
BASE COUNT         2653 a         2367 c         1998 g         3741 t
ORIGIN      
        1 tcagttgttt gaaccaataa cccaaaacaa tcattccaaa cagtaattct gtattatgac
       61 aaatgtaaaa ataacaacaa catgggatga gacggttatt ttgctctgtg caaaataacc
      121 tacgagacat cccgtaggga caacccctgt ctcgtctcat ccaatgttgc tatttttaca
      181 ttgctgacgc taagataaaa gaggcatctc tgatcttacc ccttacccaa gaatcttatc
      241 tatcttcctc ttaaactgaa cagcagttat tacgggccgc catgtgtatc ctaaatccct
      301 gattcttgct ctaacaatat ccataaccga gcggcctttc ttaaacctct ctcttggctg
      361 cctctttcct tttttatggg aaataaagtt tacaaatgcc cgcaagtcct gtgcgatgag
      421 agacatctgc ataagcaaat acctaagggc agcttttgtt gagcaggtaa acggcgaaaa
      481 gatattgtct actctgtagt ttgtttcgat gccaaacctg tatttatagg tctcgctcaa
      541 caggtaatat tgcctcatgc tcagattata catgtttgtt ataaagaaaa aaacgtcatc
      601 cttatcctgt actattatca gccgtacctt taattgatac tcttttggtc tgtcttcgct
      661 aaaagtatag tctagaatag atacgcaata ttcgtcatcc ttaatagtcg actgctcttt
      721 catgcatagc tctatctcct ttagcttatg gtttcttgtg atgggcatga tatacttatg
      781 gcagagaagc cccaggaccc gggaatactc tttattgcca aatcccctgt ccaggaatag
      841 gagcttgatt ggcacaagct ttcttatctc ccttatcatt tgctcaaatt tccccacaac
      901 agaagcaaga gaggggattg gcaggcataa taggttgaat cttactcctt cctccacaat
      961 agaagcagtc agatactgga tagcatagtt tgttccggcc tttctcttgc atcccttaac
     1021 cataaggaga tttttgtctc catagaaggg ctttgctgta atatcggctg caacgcaata
     1081 tcccttattc ttaaaagcgg cattcttcat gagcttgagc aaaagctttc ggaatatcct
     1141 gccaatctca aggccatcat ctttatcaac catgttaaga atcctatatg tgtaggacca
     1201 ggagtacagg ttctttgact ctctgtattc ttcaatgact gaatggatag atcctttctc
     1261 cgtaaagttc gccatgatca ctgggaagaa cagtttgtac agcttggttt tatcgttgtc
     1321 aaaatacaga tagatgatgt ccaggaagag gccgcaaaca gccgtatgga agttatcttt
     1381 tcttttgaac tccttggtgt acctcttaca gatctctttt gattcagttt tagagacgcc
     1441 tctaagttga aatacaaacg ccattgtgtt cacctctaag gcgcctttta acgcctataa
     1501 taataggcgt tatttttatg tttttatatt cttgagaata gaaaagctta tatataagtt
     1561 taacgcctat taaatgatgt ttgttaggaa aaagagattt aagaggggag acaagtattc
     1621 aacctattat tacttggaaa aacaggaaaa ggtaggcggt aaatttcaaa caaaaactgt
     1681 aaggtatttg ggaacagcag aaaagatact ggaaacatat gaagaactgg aaaagctaaa
     1741 gcgcaaaata gtttagttca aacaactggg tcaaaaccta cattaagaat ctactgttta
     1801 ccaccattat atcccatctt tgccaattca gcatcataat agatctgaat cctatcaaca
     1861 cattctaaag cagttgatcc attaaggtgt tgtcttgcct gcaaaaggaa ttgcgatctt
     1921 gttggctttg tttgactttc aacaaatgct tctcttgcag ctctaaataa atcatctgca
     1981 agtttatcta ttgctgtttc aggtgatact aacggtggtc ggcaatctct catagcctta
     2041 gcaaattaat atctatttaa aaaattatcc ctcgctaatt accttatcca gccttatatc
     2101 ccaatcatct cttggtatgt cctcaaggac ctgcagttcg aatgctaagc ctatcttttt
     2161 tccgctaaac tcttttagga aatcatcata ataaccctta ccatatccaa tgcggtggtt
     2221 atgcttgtca aagacaaccc ctggcactat cacaaggcca atcacatctt tgcttacttc
     2281 ttttatcttc tctggctcgc aaataccata aacgtttttt ttactaagct cacctgggct
     2341 tgctattttt gaagcgacca acctgtcttt tatgacgcaa ggaacacaca ccttcttttc
     2401 ttctagcgac tctttaatca tgtcccatgt gtcaacctca gagccaaagg aaacatagaa
     2461 catgactata tttgcttgcc tatattcttt tgagttgaaa agaatttgct taatcttatc
     2521 tgacttttcc tttatctcgg ttgcggatag ccggtttctt cttcgcagaa taactctgcg
     2581 gttgatattt ttcatctata aatatctatc acgtattaag ttaataaaat ttgtcattta
     2641 atcatgcttt cgcttgtgct tattatctgg ttgaaatctt catcatttgc ctgatacttg
     2701 gcaaactcca ccatactgca aaggtttagg catttttggg tatcctcatg gcttattttt
     2761 tctttcttta agtgttttat aagctcagaa ttagtcaact ctatcctaat gcctaactta
     2821 tggctataga agaaacggac tgcttgagag gctttttcgt atgcatcctt atactttttt
     2881 tctctgtata gcttttttgc ttcatcaagc atatttcttg actctttcct ataatctata
     2941 ggtctttcta taaccgcatg aggcatttga tcggctgatt ttctaaggta ctttgtgtat
     3001 gcaaaatata tgccaattgc taatagtgga agggataaag cccatatcca ccaaaggctt
     3061 ttttcttttt ccaactttat gtcttcaact acattatttt ttatctgcgc agttattgca
     3121 gcagttttgt tattatcgtc ttgataggga atcttgatcg aggttgcatt tcctgcagtt
     3181 tgaacctccg gctgcttggg agtatatcct tgggattgaa gatattgaga gtattgctga
     3241 tacctttggt cgtttagcag ttgatccatg atttttttga tatcttcgct tgtagttttc
     3301 tgaatttctt tcatctgatt attttccatg cttcctttaa ctgtcgctgt ctcagctccc
     3361 ttttcataat tgacttcaaa attgccagaa gtgctgtttg tcgggtcaag gtttccggac
     3421 atgggcttat atccttgctg tgtgagatcg ttatgctttt gctggaactc cggattctgg
     3481 ccaaggttgt tttggaattc ctgcttcatc tgctgctgct cctgcatctg tttttggagc
     3541 tgctgcttta atgctgagct gtcttgcgcc atctggttgt tctgcacagc ctgcatgggg
     3601 tctggcttgc tttgctggct gccaaatatc ttgttcatct gctcctgcat ctgctgctgc
     3661 agttgttgca tctgctgctt tgcttgctgc atctggctct gctgactttg ttgattgctt
     3721 tgctggctgg tttgttgctg attattttgc tgcttgcttt ctttcctttc gtcattcttt
     3781 tgcttttgag actggtcttg tacaaagtat actattatcc ccctgagatt tatatttacg
     3841 ctgccataag caaatgtaag gtctatcgta agttttatcg gtttgtcaga agtgcctgca
     3901 ctaaaagtca ctatctgttt tcctcctgga gctgtaaatg gctgagaacc actgtttgag
     3961 cttgaatact gaaagcctgc ttggcttatt tcctgagtta ttgtatagct gagtgttcct
     4021 tgaatagggg ttgacgaagg gttgtcaaac tcaattggaa taattgcatt ctcattaaca
     4081 aggaaataat attctgtctg atttactgca agtgtaagct cttttgcagt gcttgtggtt
     4141 gaaagaaccg ctaacaataa tgagaagacg ataactgttt taggggtcat tttactggat
     4201 aatcctattt cttccatacc ttaggtaaac ttcgacgagc aggagcatca ccgctgagcc
     4261 aaaaaaccaa tccttgatgc ttgtgttctc tttttctcgc tttatgtcct tgctaatatt
     4321 tttataaata tcactcaggg ttttttcatc cactgactta aaataagagc cgcctgtctg
     4381 ctgtgctata tcttttagcg tctcttcatc aagctctgca tattgggggt ttccaaacca
     4441 atcatagccc aagacaacgt ttccttctga gcccatggct atcgcatgaa cctggatctt
     4501 gtttgatttc gcaaactgga tagcttcctg cggtgagatg actccggcat tatttacgcc
     4561 gtcacttagc aggacaacga ccttcttctt gtttggaatt gatgttgcca tatctattcc
     4621 caggcttaag ccatcgccta ttgcagtctt tccttccctt gcagtaatcc ctttaagctt
     4681 gtccaatgcc ctgtccttga atggcgtaag gtagcatgct gttgtcgcac cattttcaaa
     4741 tattacaatg ccaacatggt catttggctt taagctttct atcagaatag ctgctgagtt
     4801 ttttgctgcc tcaagcctgt ttggcttgta gtcggttgcc tgcattgagc ctgagtcatc
     4861 tatcacaagg acgacattga ccccttcttt tgtctgcttc attggaatgt gaggatctgc
     4921 aaggcccatg agcatgagtg caatggctgt gagcagaatg tagaacaata gttttaccct
     4981 tatgggcttt ttatcaccca tggctttttt aattatggag agatggctga actttatcgc
     5041 ttcctttttc tttttttgat cggcaaggcg atggaagaaa aaaatgacag gtacaattag
     5101 aagaaaccat aacagatgag gatattggaa gctgaaagcc atatttatct taccatcctt
     5161 ctttctctta agcgaaagaa tttcctcaat ggtatctcat aaggctcttg gcttttcagc
     5221 tggatgttgt ctatctttag cttttttaac atattattca gacggtcatt tctttctttc
     5281 atcatggaaa tatatgattg cctgaactcc ttgtcggatg tatccagcag gagctgctcc
     5341 cctgtctctt catcctccag ttctatatat ccgacgttgg gtatttcgct ttcccttatg
     5401 tccgacatgt ttatcgctat cacatcatgc ttgtttttta ggatctttag ctctttttca
     5461 aaggaatctg tgaagaaatc agagatgata aaaatgatgc tccttttttt aatgactttt
     5521 gacaaaaacc ttagggtttc tgcaagacca gtagctttgc tttttgggtt gtattctatc
     5581 atatctctta caaggcgcag aatatgggtc tttccttttc ttggcgaata gtatttttca
     5641 gccttgtctg tgaaaaggca taggcctact ttgtcgttat ttctgagcgc tgcaaacatc
     5701 aaggatgcag agagctctat tgctgcttct ttcttgcttt tttcggagcc aaaatcgttt
     5761 gatgcagaca cgtcaagaac tatatatata gtaaggtcgc gctcttcgat aaactctttt
     5821 acatagggat ggttcatcct tgctgtgaca ctccaatcaa tggagcgtat atcatctcct
     5881 gcgacatatt cacggacttc tgagaattcg atgcctcttc ccctaaatac agaatggtat
     5941 gcgccagaga gaaggccgtc tacaagatgc tttgtcttta tctctatctt tttaactttc
     6001 tttaatatct ctatagtctc tggcattttt acggctagtc tcttaacatg atatctgctg
     6061 cttgcttgat gtttgaaact tctcttagtt ccaggcccca ctcttttaca ctttcggtta
     6121 tgtttaatac ctttggaacg tatcgcgtat tataaaaaac aaaaccaagg cctgtctgtt
     6181 ctctagtgac ctctcgttcg taatatctta caactgtctg gcctcttggg accaggaaaa
     6241 gcttataacc tgcgtctgag gctgcttttg ctttctcaag gatgcctccc accctgccta
     6301 tcgtgccgtc gctgtttatg gtaccagtta ttatcgcatc ctgccttatt gttttattat
     6361 ccaatgctgc tatggctgca agggttgtag ctgcacctgc actgccgccg ccaacaacct
     6421 ggctttcaat actgtaagtg aaaacaaaat ccctatcagg attggatttg ccagtaactt
     6481 ctattgccac ttcttttgcg atatttgcgc tgtactgcaa gtcaggctca aggaaggggt
     6541 ttgtttcgat aaggacattt gaattgccag gtataagccg cacagtaagg ttgcctatga
     6601 tgccctcgtc ttcgtcttca ctgactgcaa ctattgccag acttcgctcg atagtctggt
     6661 ttataggccc gatgcaagtc gtgttctggg cgtcttgttc ctgtatcgcc tggcctgtca
     6721 ctttttctgc agtaggatag ccaaatatcg ctacagctac gatgaatccc agcacaaaaa
     6781 ataacatagc gtttgcaacc ttgctcatgg aacctttact ttttcgatta tcttgcttat
     6841 tatatcgtca gaagttatct cctcagcctc tgcttcatag ctaagtatta tcctgtgcct
     6901 gagcacgtca tgtgcaacag ctttgacgtc ctctggcatc acgtaacccc tctgatgcaa
     6961 cagagcgtgt gcttttgcag ctaatatgag ccagattgaa gcccttggag atgcgccata
     7021 ctcgatatgg ccgtcaaagt ccagatgata ctccaaagga tgccttgttg catcaactat
     7081 cgaagacaca tactcttcta tcttctcatc agcatatatc tgctggttga atttttgaat
     7141 ttttattata tcatctgggt ttataacttt tgccaaatca aactctctcc cctgagtatt
     7201 tctcctgatt atctccatct cttctttttt gctggggtac tttatcaaga gcttgaacat
     7261 aaacctatca acctgcgctt caggcagctt gtaggttcct tctgtctcaa tgggattttg
     7321 ggtcgcaagc acaaggaatg gcttttccag cttgaatgtg tccccttgga ttgtcacctg
     7381 acgttcttgc atggcttcca ataaggctga ttgcaccttt ggaggagccc tgtttatctc
     7441 atctgccaag atgaagttgg taaagatagg gccttttttt gtcgtgaaag tccctgtttt
     7501 atgctcataa atctttgttc ctataatatc tgcaggaagc aagtcaggtg tgaactgtat
     7561 cctgctgaat cttgagttta atgtttcagc cagggttttt atcatcaaag tctttgcaag
     7621 gcctgggact ccttcaagca ggacatggcc gtcagcaatc agggagatca tcagcttttc
     7681 aaggacctct tcctggccta ctatgacttt ctttatctct tcaagcgcct ttactatctg
     7741 cttgttgtac ttttctgctt gtgcgtttag aagcttaatc tctttgtcca tatgtcctct
     7801 cacccctcaa atgatagaat ggacacctat aaataactta tttaaaaaaa ttatggaaaa
     7861 gaactcttga aagatccaat ctttgtttga attttgcact tgatttccaa tttttttcga
     7921 tttcgatagg taaaagcgcc aacttaaagt atttcttcag gttcaatacc caagcgataa
     7981 caaaagaatg caaaccaata gtttccaccg acaaggcctc taagttctga ttcctttttc
     8041 ccgcacagtt gacatgtata cttatatttc taaaaaaaac atccaggcta ctgttcattc
     8101 ttatgataca aatccagaaa tacaacctta ttttcctgct ttagataaat atatgacaat
     8161 ctgcaaggag atacatacaa ctccctggtg cctttcctat cgaacctcat gggcttccca
     8221 atctcgggat tttctacaat ctttgctatt tgcatcttaa ctttttcttt tgttacaggg
     8281 tccttgattt tgcaaataag cttcaaaaaa tgaggttcat aaactacact tagcattttt
     8341 caatcatctt tagaaattcg cttgcaggaa ctgtcttaaa atctccgctt tcataccttt
     8401 ttaaagcctc ttctgtcctt tttgcaaaaa ttaaatcatc tttaaggttt tcatcaagct
     8461 cactggcttt ttttatgata attctgctat catttttaat tagcaataat ttgtctcctt
     8521 cgtcaattcc aactctcatt tcagaaggta taactatctg accttttgaa ctcatccttg
     8581 taaatgctac atccataata atcaccttgt aagattaatc ttactaagta tataagcttt
     8641 ttgtattgct taattgcttt tttgcctaag tctttgcata tgctttatcc ttttatcgat
     8701 aaggtttttt gtcccgatat cttccctgtg aaagataggg ccttttgcag cctttactcc
     8761 ttcttcagca atattttgag cttcttctat gtcctttgca atgcccacaa atgcaagagc
     8821 ccttgacttt gacatgtaaa gcccatcctc cctcttatct atagatgcat aatatacctt
     8881 gacgtttttt ggaacattat ccaatgaaac tttttggttt tctaccggat ggtctggata
     8941 gccgtttgga actgcatact tacagactgt cgatatattg cggaagtcag cttttatctt
     9001 gctcagcgta ccatctgcta ttgcctggag gacatctaca taatcattgt tcattatcgg
     9061 caagacgttc atcgcttctg ggtctccaag ccttgcatta tactcaataa gctttgtccc
     9121 ttcttttgtg cagatgaagc cgccgtacat gactcctttg tatggctcac cgcattcttt
     9181 ttttagggct tctctcatct tatctgttat ctcctttgcc tgctctaagt ccgatttttc
     9241 catgaaggga agaagctcgc cagttgagta cgagcccatg ccgcctgtgt ttgggccttt
     9301 atctccggca aatgctcttt tgtgatcttg gacaggatga cagtgaagca aggaaaatcc
     9361 atctgtgaag ctttggagcg agaattcttc tccttcaagc ttctcttcga caacgacagc
     9421 gctgtgtgac tttagcactt cctcacaata ttggtaagct tcttcgactg tggtgaaatg
     9481 atcgccttgt actttaactc cttttcctgc tgtaaggcca tctggcttta gcactatttt
     9541 tggatggaga tcgatcagaa actctttcaa tcctttggtg ctattaaaga ctccaaatat
     9601 cggagcgcct ggaacattat atttcttcat gagatttctt gtgaaagatt tagaggtttc
     9661 aagctgggca agctgttttt ttgggcctac tgcctttatc ccttcttttt ccaaggcgtc
     9721 aacgactcca tcagatagcg ggtcttcggg gcctatgact gcgatgtcta tctttgaggc
     9781 ttttgcataa gatgttatgg tgcttaggtc atcatacttt ccaagcttta cctgatatga
     9841 gagagaagca atacctggat tgtttgcctt catataagaa taaagctttg gcttttttgg
     9901 gctcctaata atcgcctcag ccatcgcatg ctcccttgcg ccattaccta tgagtaagac
     9961 attcattgta aacctcctgc tggtatctgt tgagctgcat ctggatgctt ttgccagtcc
    10021 agcaataagt gcaaattttt tcttttgcta ctccgatggc tgatatcata tcatcaagct
    10081 ttaggtattt taaagagttg actcctaggt cttttgctat ccaatcgacc atcttcttgt
    10141 acttttcgga ttcagggttc aaatattcag agatatcttc tatctccttt ccttctatcg
    10201 cccttatagc ccgccttgcg acaagctcat ggttggtcct agtggaaagt gcatagatgc
    10261 atggaaacaa aagcggaggg caagctggcc ttatatggat ctcttttgcg cctgcttttt
    10321 tcaatttggt tatggtataa ttttttagct gggttcctct tactatggaa tcctcacaga
    10381 gcaggatgcg gttatccttg atcacatctt cattggcgat cagcttcatg agcgctatcc
    10441 tatctctaag atcctgagtc aacggggtat aactccttcc atatcctgat gtatatttta
    10501 tcaatgctct cctgtagggc tttccagatt ggatggcata gcctatggca tgagcggttc
    10561 ctgagtcagg cactcctgag acaagatcca cgcctacatc atcacttttt gccagaagct
    10621 ttccacaatt ttccctgact tgctctacgt ttatgccttc atatgaggat gctggaaatc
    10681 ctgtgtatat ccaaagaaaa gcgcatacct tactttcatg attaccctgg ttatgtatct
    10741 cgatgccttt cctgctgat
//
LOCUS       DASRUK010000041         2548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_876542, whole genome shotgun sequence.
ACCESSION   DASRUK010000041 DASRUK010000000
VERSION     DASRUK010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876542"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1966)
                     /locus_tag="VFF28_01860"
     CDS             complement(<1..1966)
                     /locus_tag="VFF28_01860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HZX44408.1"
                     /translation="MAENYHALTTGEVLALFSTSKQGLSEEKAKARLQEFGLNELQKE
                     KRSNAFFLFVGQFRNALSILLIFAGVLSLFTSDKLEAIAIFSILFINSTLGFIQEYRA
                     EKAMEALEKISAPSARVLRDGKQRKIPARDLVPGDIIMLEAGDIVPADSRLLDVASMY
                     IDEASLTGESVPSKKFIEPLKNEITVADRENMAYTGTIVTYGKAKAIVVSTGMNTEFG
                     KIAASIQETEEPETPLQNKFAQLAKQIGFVVMGLIIVVLAAGTIQKTVSFAGMVLFAL
                     ALTVSTIPSSLPIIVTVSLSMGAKRLSRKNMLIKKLPAAESLGAATIICSDKTGTITK
                     NQMTITHIYSNDKVINVTGAGYEPKGEFKVGNKTIDSKELELLLKIGYLCNNAKLEYV
                     NGKHELIGDPTEGSLIVLAKKGELDEKAISKRCVMIKEHPFDSERKRMSMIFKNEKRP
                     EAYVKGAPDLLLEVCSKMIKNSKICPLTAKEKKKILDANNKFAEQSLRVLGLAYKDLS
                     SRKRYTLEEVEKDLIFIGLVGMMDPPRDEVKDAVKQCHEAGIKVMLITGDHAVTTKAI
                     AKNIGLFSKGDLVMTGEELDKMTDDALEKQIDKIRIIARALPIQKSRIVDILQKKGHI
                     VAMTGDGVNDAPALKKSDIGISMGVIGTDVA"
     gene            complement(1968..2165)
                     /locus_tag="VFF28_01865"
     CDS             complement(1968..2165)
                     /locus_tag="VFF28_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44409.1"
                     /translation="MQTVEFKRKLYKRGSSYETTIPMPLLFALDKDKKYNVVFFFDPE
                     ANKWYLKFEENLSSIEEKIEG"
BASE COUNT          620 a          576 c          494 g          858 t
ORIGIN      
        1 ttgctacatc tgtaccaata acacccatag agatgcctat gtctgacttc ttgagagcag
       61 gagcgtcatt gacaccgtcg cctgtcatcg ccactatatg gcctttcttt tgcaggatat
      121 ctactatcct tgatttttgg atgggaagag cccttgcgat tatccttatc ttgtctatct
      181 gcttttcgag cgcatcatca gtcatcttgt caagctcttc tccggtcatc accagatcgc
      241 ctttactgaa aagcccgatg ttcttggcta tcgcttttgt agtgacagca tgatccccgg
      301 ttatcagcat aacctttatg cctgcctcat ggcactgctt gactgcatct ttaacttcat
      361 ccctgggagg atccatcatg cctacaagcc cgataaagat caagtctttt tccacttctt
      421 ctaatgtgta ccttttcctg ctagaaaggt ctttgtatgc aagcccaaga accctcaaag
      481 attgttctgc aaatttgttg tttgcatcaa ggatcttctt tttttccttg gctgtaaggg
      541 ggcatatctt actgtttttt atcatcttgc tgcaaacttc aagcaatagg tcgggtgctc
      601 ccttgacata ggcctctggc cttttttcat ttttgaatat catggacatc cgcttccttt
      661 ctgaatcaaa gggatgctct ttaatcataa cgcatcgctt gctgatagct ttttcgtcca
      721 gctctccttt ctttgcgagg actatcagcg aaccttcggt gggatctcca ataagttcat
      781 gctttccatt gacatattcc agctttgcgt tgttgcagag atagcctatc ttcaatagaa
      841 gctcaagctc ctttgaatca atagtcttgt ttcctacctt aaattctcct ttgggctcgt
      901 aaccggcacc tgtgacgttt atgaccttat catttgaata gatatgggtt attgtcatct
      961 ggttttttgt tatcgtgcct gtcttgtccg agcatattat cgtcgctgct ccaaggcttt
     1021 ctgctgcagg cagctttttt atcagcatgt tttttcttga aagccttttt gctcccatag
     1081 aaaggctgac cgtaacaatt atcggcaatg agctgggtat cgttgaaacg gtgagggcca
     1141 gggcgaacaa gaccatgcct gcaaaggaaa ctgttttttg gatagtgcct gcagcaagga
     1201 cgacaattat tagccccatc accacaaagc ctatctgctt tgccaattgc gcaaacttgt
     1261 tctgcaaagg tgtttctggc tcttctgttt cctggataga tgctgcaatc tttccaaact
     1321 ctgtgttcat acctgtcgaa actactattg cttttgcctt gccgtaggtg actatggtcc
     1381 ctgtataggc catgttctcc ctgtctgcta cagttatctc attttttagc ggctcgatga
     1441 atttcttgga aggaacagac tcacctgtaa gagaggcttc atctatatac atacttgcaa
     1501 catctaataa ccttgaatct gcagggacaa tgtctcctgc ttcaagcatt ataatatcac
     1561 cagggacaag gtctcttgca gggatctttc tttgctttcc gtctcttaga acccttgctg
     1621 aaggggccga tatcttttca agcgcttcca tggccttttc agcgcggtat tcctggataa
     1681 atccaagtgt cgaattgatg aacaagatgc tgaagatagc gatagcctca agcttatcag
     1741 aggtgaacaa tgaaagcact cctgcaaaga tcaaaaggat gcttagggca ttcctaaatt
     1801 gacctacaaa aaggaaaaag gcgtttgacc tcttttcctt ttggagctca tttagcccaa
     1861 attcctgcag ccttgccttt gctttctctt ctgagagccc ctgctttgag gtgctgaaga
     1921 gcgcaagcac ctcgcctgtt gtaagtgcat gatagttttc tgccatatta tccctcaatt
     1981 ttttcttcta tagacgataa attttcctca aattttagat accatttatt tgcttcagga
     2041 tcaaaaaaga agactacatt gtatttttta tctttgtcca gggcaaagag aagaggcata
     2101 ggaatagtag tctcataact agagcctctc ttgtaaagct ttcttttgaa ctcaactgtc
     2161 tgcatttttt tgcatatagc aaaagactca ttattttctt ttagtataca ctaggtcttt
     2221 tgcttttacg aaaagataaa aatcttcaaa atatttttat ctttagtata cttacttatt
     2281 tacttatcaa tatattcatt tttatactta cttatatata aagattatgg ctaaagcaag
     2341 aggggatgac taaagtcagc tctcagtatt gagaatctac ttgcctctgt agatagggca
     2401 tagtttacat tcagaaacaa caagatcagg ggctatctcc tgcgggcaat cgggaaacgt
     2461 gcctttgcag cctggatatt tagttgtggt cctgaactca tcctgctctt ttgcccattg
     2521 gggctttgga acataagact gtttatgg
//
LOCUS       DASRUK010000042         9717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_545164, whole genome shotgun sequence.
ACCESSION   DASRUK010000042 DASRUK010000000
VERSION     DASRUK010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 9717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9717
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545164"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="VFF28_01870"
     CDS             <1..1160
                     /locus_tag="VFF28_01870"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF016844.4"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A'"
                     /protein_id="HZX44410.1"
                     /translation="QLISPRYGLSIIGCVQDAISGNYLLTKALDLPRPEAIDLLTFAG
                     VTNYSKLPRKEKITGKEIFSVLIPDDFNFTGRARHYDAAQSKEENTKRDAYVVIKDGK
                     LLSGIMDRNNLGEGSGLLLRNIHKRYGEEKAIEILGNLFRLGIGVLLKVGFTTAISDT
                     DLPESVSQSIRDTLNAAKKEVDELIEQFNNNKLDAFPGKTVKETLELKILEVLNRARN
                     KTGELVSKNANKKCHTLVMADSGARGNLLNLAQMAACVGQQAMRGKRIEKGYKGRTLS
                     SFKKGDLSPAAHGFIANGFKTGLTPYEFFFGAMTGRDSLMDTALRTPKSGYLYRRLAN
                     AMQDLKAEYDETVRDAAARIIQFSYGEDGIDVSKSEGGILNVKRIIRTVGE"
     gene            1162..2289
                     /locus_tag="VFF28_01875"
     CDS             1162..2289
                     /locus_tag="VFF28_01875"
                     /inference="COORDINATES: protein motif:HMM:NF016859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A''"
                     /protein_id="HZX44411.1"
                     /translation="MSIFTQYKEKFPKKVYDEIKELTKGMSEAKQKKILEACLEEYNK
                     TKVEAGESVGVVSAESIGEPGTQMTLNTFHFAGVAEMNVTMGLPRIIEILDGRSEIKT
                     PMMEIYLNAPYSQGKDIRKLALSIKETLLAEVIKEFVINIADSSIELNLDDTKMAELG
                     ITETVLSNALKGGVKGYNVKITKDSIVLKSKTKEESVNKVYVDKEKIKGIHLKGVKGI
                     SQVLPIRRGEEYIIITSGTNLKEINSLDFVDAARTTTNDIFEIQKILGIEAARQSIIN
                     EVYKVIESQGLDVDIRHIMLVADTMCNAGEVKGITRYGIVSEKSSVLARASFETPIKH
                     IVSAALIGEIDKLTSVVENVMLNQPVPVGTGLPNLVTQVKK"
     gene            2289..2552
                     /locus_tag="VFF28_01880"
     CDS             2289..2552
                     /locus_tag="VFF28_01880"
                     /inference="COORDINATES: protein motif:HMM:NF013417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HZX44412.1"
                     /translation="MVESDKEMGEIRKLLKSKSMIIGTDRTLKQLKLGKIKKVYVSAN
                     CDQKTLDTISRYDAEVVKLSYPNDELGILCKKTFSISVLSVPK"
     gene            2549..2959
                     /locus_tag="VFF28_01885"
     CDS             2549..2959
                     /locus_tag="VFF28_01885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusA-like transcription termination
                     signal-binding factor"
                     /protein_id="HZX44413.1"
                     /translation="MKIKYDLTVMKYMQLFENLTRAKLHDCINDEQLIFVVEENEIGK
                     AIGKHGSNVHRLEGLLNKKIKIVEYSSDVRQFIRNFIMPLSVGDISEENNIITITGPD
                     TKTKGLLIGRERKNLLYLKDIVSRYFQVEDIKVV"
     gene            3003..3440
                     /locus_tag="VFF28_01890"
     CDS             3003..3440
                     /locus_tag="VFF28_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010885620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HZX44414.1"
                     /translation="MAKKSRGLNAGSKLKKRRQEGRWHSDEYVERMKNLKAKSDPLGG
                     WSQAKGIVLEKVQFEAKQPNSAMRKCVRVQLIKNGRQITAFCPGDGATKLIDEHDEVM
                     VESIGGAKGKSKGDIPGVRWQVIKVNDQSLIALRKGIIEKARK"
     gene            3437..4036
                     /locus_tag="VFF28_01895"
     CDS             3437..4036
                     /locus_tag="VFF28_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014557733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HZX44415.1"
                     /translation="MSEILAFNRWTTEGISVADAGLQRYITLEPKIVPKTGARYHGNK
                     FHKSRVFIVERLINKVMIPGHKSKKHVKSSWKLTGKAHTAYDIVEDAFKIVEKQTKEN
                     PLKVFVKALENAAPREEIITIEYGGARYPKAVECAPQRRIDIALRLMTQGAYHKCFNS
                     KRGIIECLSDELINAYKMDRASNAISKKLELERQSDASR"
     gene            complement(4039..4851)
                     /locus_tag="VFF28_01900"
     CDS             complement(4039..4851)
                     /locus_tag="VFF28_01900"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HZX44416.1"
                     /translation="MQYMLAPIEDITSLSFRILACRHGCDVAFTELIRFESLARNNKS
                     TWERIEFSAKAPTVIQVIGQREMWLKKFLSSFVPGEGFKGFCLNLGCPAPNFVNQGVG
                     AAMIKRISKVNKLIDVVKSRGYPVGVKMRLGLNQFEKDKKVYLNLLNSTNADYYIVHA
                     RHGKESYNVPADWSVFPECVATGKKIVANGDVKTVSDVKKLETMGVVGVMIGRAAVTN
                     PGIFDTLKGKESASLDSLRKEYESLSANEPFRYKKNVLKWMGGKSLTEEKIS"
     gene            complement(4998..5288)
                     /locus_tag="VFF28_01905"
     CDS             complement(4998..5288)
                     /locus_tag="VFF28_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44417.1"
                     /translation="MIIKIASLNDLAKLGHAPGRIEEELRKGGYDSPIIHYAFHHTTN
                     DKERLSHLRWSGMPGKVWYSGIYLPDGEPLEERLTQTPEGHADALAAVFFAL"
     gene            5365..5982
                     /locus_tag="VFF28_01910"
     CDS             5365..5982
                     /locus_tag="VFF28_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0090560 -
                     2-(3-amino-3-carboxypropyl)histidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0017183 - protein histidyl modification to
                     diphthamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthamide synthesis protein"
                     /protein_id="HZX44418.1"
                     /translation="MKTLFIEARYKGKVVLPKEVIEKLPKKIGLASSIQFIGSLKGIS
                     SQLGKGSLIAGQILGCDAKSAGKIAERVDSFLYIGDGEFHPIAIALETKKPVYKYNPK
                     AKTLTQLDKAKIDSYERKKKGAYLKFLNAKKVGILLTTKPGQRISDGKLKQLEQKFKD
                     KKFYRFIADTIDYTQLENFPFIEAWVNTACPRIEEDILALNISEL"
     gene            5979..7202
                     /locus_tag="VFF28_01915"
     CDS             5979..7202
                     /locus_tag="VFF28_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HZX44419.1"
                     /translation="MKPELLAPVQDFTSLKAAIDAGADAVYFGIKELNMRLGANNFTK
                     GQIKKVIDICHKNKVNAYFTLNSIIYENETRRINHLLAILKKNKIDAIIAWDFSVISE
                     CKKLSLPVHLSTQASIASFQAAKAIKKQFSNIKRINLARELSLSQVRSIIKQIKQHHL
                     DIEIECFIHGAMCVSISGRCFLSQDIFNKSANRGECLQPCRRKYIIKDIEEDHEFELG
                     SDYIISPKDLCTINIIDKLIKTNISALKIEGRNRAPEYVKTAVECYRKAIDAAINGKL
                     TQSIKDELEKKLSTVYNRGFSTGFYLGQPANGWSKAYGSKATTKKILIGFVKNYYKKI
                     GVAEVKIESGNLKLGDSIMFQGPTTGVVEQKITSMEDSKGKTNKAAKGQLVTIKSDHL
                     VRENDKLFLIRPCLS"
     gene            complement(7179..>9717)
                     /locus_tag="VFF28_01920"
     CDS             complement(7179..>9717)
                     /locus_tag="VFF28_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZX44420.1"
                     /translation="AACFDDHGHGTHVAGTIASNNTTYKGVAPSAKIIAIKALNSAGS
                     GWSEDIIAGIDWCTYNATKFNISVISMSLGDGKAYQTYCNDEPETASINAAVAQGIVV
                     TVAAGNCDGVACTAGISAPACIQNATAIGAVNDADAIQYQRGALFELLAPGLNIMSLE
                     YLSDGFLAMGGTSMATPHVAGAVALLKQFTKLQDGTSASPSSIRAALNSTGKRIDDTS
                     GSGYLFSRIDILKAVRSLDAVAPVWSGNSSNTTRPQRNGTVQFNISVNDTVGLSGYIF
                     SINETGSWVNSTNGTLDGKIQLISINKTISNNSRKNIAWKFYVNDTNNNWNFTDEWAL
                     LLDNRIPEVLNLSLNSTDILNRTNGSLVANFSFFDLDEDLLQLNETRWYNGSVYVSSL
                     DNATIIGSGNLTRNDTWIFSVRVFDGDNWSIWYNSSSIRIAGPFTSPSLFSVADYDAD
                     GNIELNWSDDGNETGESYRIYRHTSNISAINSSLSLIASSVPNGTQYYEDNDTTHGTT
                     YWYSLITVDSLGNFNTNVFSYSLNGTANDTIKPKIPRVFNATSSSGTTTLNWSYVSQD
                     VAGNLDNFSLSYKIWYKIATSVNLSKGYVNETADYIKTVSSCADGYCTTTHSLSSSSV
                     HYYFVTTIDDAGNENLTLDNSSLGNLINMTATTAPSNAVTTSSGGGGGGGGGGGGGGG
                     GSSSKTNLDVKEGFVSGSGFSVKQTQGDEATFTFGGVKHTIKVVTLQADGISLSIDNK
                     SKVPLKENQGKEIDVNNDKVGDVAVTLKKIDGGRAEMLYKMLIKPAPKAEENKTVESQ
                     AAAVQEPTVQVTESLPPKKNNWFRFVILSGSAFAFAAYMVLRKTRPN"
BASE COUNT         3159 a         2047 c         2062 g         2449 t
ORIGIN      
        1 ctcagcttat ctcgccaaga tacggcctaa gcataatcgg ctgcgtccag gacgcaatct
       61 caggaaacta tctgcttaca aaagcactag accttccaag accagaggca atcgatcttt
      121 tgacatttgc aggagttaca aactactcaa agcttccaag aaaagagaag atcacaggaa
      181 aagagatttt ttcagtcctt atccctgatg actttaactt taccggaagg gcaaggcatt
      241 atgatgctgc tcaaagcaag gaagagaaca caaaaagaga cgcttatgtt gtcataaaag
      301 atggcaagct tctttccgga attatggaca gaaacaacct tggagaaggc tcagggctat
      361 tgctcagaaa catccataaa agatatggag aggaaaaagc aatagagata ctgggaaatc
      421 ttttcaggct gggaataggt gttctattaa aggttggatt cacaacagct atctcagata
      481 cagaccttcc tgaatcagta tctcaaagca taagagatac ccttaacgca gcaaagaagg
      541 aagtcgatga gctaatcgag cagtttaaca ataacaagtt ggatgcattc cctggaaaga
      601 cagtcaagga aacacttgaa ctgaagatcc ttgaagtgct caacagggca agaaacaaga
      661 caggagagct tgtctcaaag aacgcaaaca agaaatgcca cacccttgtc atggcagatt
      721 caggcgcaag aggaaatctg ctaaacctcg ctcagatggc agcctgtgtt ggccagcagg
      781 ctatgagagg caagaggatt gaaaaaggct ataaaggtag gacactttca tcgttcaaga
      841 agggggattt aagtccagca gcacacggat tcatcgcaaa cggctttaag accggcctta
      901 ctccatatga gttcttcttt ggagctatga ctggaagaga ttcgctgatg gatactgcgc
      961 taaggacgcc aaaatctgga tatctataca gaaggcttgc aaacgccatg caggacttga
     1021 aggcagaata tgacgaaacc gtcagggatg cagcagcaag gattatccag ttcagctatg
     1081 gcgaagatgg gatagatgtt tcaaaatcag aaggcggaat actaaacgtc aagagaataa
     1141 taaggacagt tggtgaataa gatgtcgata tttacacaat acaaggaaaa gttcccaaag
     1201 aaagtttatg acgagataaa ggaattgacc aagggcatgt cagaagcaaa gcaaaagaag
     1261 atcctggaag cttgccttga agagtataat aagacaaagg tagaggcagg cgagtctgtc
     1321 ggagttgttt ctgcagaatc tataggcgag ccagggacgc agatgacgct taatacattc
     1381 cacttcgctg gagtcgcaga gatgaacgtt accatgggcc ttccaaggat tatcgagatt
     1441 ctcgacggca gaagcgagat caagactccg atgatggaaa tatatcttaa cgctccatat
     1501 tcacagggaa aagatataag gaagcttgcc ttgtcaatta aggaaacctt gcttgcagaa
     1561 gtcatcaagg aatttgtgat taatatcgca gactcatcca tcgagcttaa tcttgatgac
     1621 acaaagatgg cagagctggg aattaccgag acagtcctta gcaatgcctt gaagggagga
     1681 gtcaaaggat ataatgtcaa gataacaaag gattcaatcg tcttaaaatc aaagaccaag
     1741 gaagaaagcg taaacaaggt ctatgtcgat aaagaaaaga taaaaggcat acatctaaaa
     1801 ggtgtaaagg gcatttcaca ggtccttcct atcaggagag gagaggagta catcatcatc
     1861 acctcaggaa caaacctaaa ggagataaac tcccttgact ttgtcgatgc cgcaaggacc
     1921 acgacaaatg atattttcga gatccaaaag atacttggga tagaagcagc aagacagtca
     1981 ataatcaacg aggtctataa ggttattgaa tcccagggcc ttgatgttga catcaggcat
     2041 ataatgcttg ttgcagacac aatgtgcaat gcaggcgaag ttaagggaat aacgaggtat
     2101 ggtatagtct ctgaaaaaag ttcagtcctt gcaagggcaa gctttgagac gccaataaag
     2161 cacatagtga gcgcagcact tatcggtgag attgataagt tgacatcagt agtggaaaac
     2221 gttatgttga atcagccggt gcctgttggc acagggcttc caaacttagt gacacaggtg
     2281 aagaaataat ggtagaatca gataaggaga tgggagagat aaggaaattg ttgaagtcaa
     2341 agagcatgat catagggact gacaggacgc taaagcagtt gaagctaggc aagataaaga
     2401 aggtctatgt cagcgcaaat tgtgatcaaa aaacgcttga caccatctca agatatgatg
     2461 ctgaggttgt aaagctaagc tatccaaatg atgagctggg catcctttgc aagaagactt
     2521 tttctatttc tgtcttgagc gttcccaaat gaagataaaa tatgacctaa ctgtcatgaa
     2581 gtacatgcag ctctttgaaa accttacaag ggcaaagctg catgactgta tcaacgatga
     2641 gcagcttatc tttgtcgtcg aagagaacga gataggcaag gctataggca agcacggctc
     2701 aaacgtccac aggcttgaag gattgttgaa caagaagata aagatcgtcg agtacagctc
     2761 cgatgtaagg cagttcatcc gtaactttat catgcctctg tcggtcggcg acatctcaga
     2821 ggaaaacaac attatcacta tcacaggccc tgacacaaag acaaaagggc ttcttatagg
     2881 ccgagaaaga aagaatcttt tatatctaaa agacatcgtc tcaagatatt tccaggttga
     2941 ggatataaag gtggtataac caaaagcttt ttaaatcatg aggaaattct tttaaagtaa
     3001 aaatggctaa aaaatcaaga gggctaaacg ccggaagcaa gcttaagaaa agaaggcagg
     3061 agggcaggtg gcacagtgat gaatatgtcg agaggatgaa aaaccttaag gcaaagtcag
     3121 acccgctcgg cggatggtcg caggcaaaag gcatagttct agaaaaagtt cagttcgagg
     3181 caaagcagcc aaactcagct atgaggaaat gcgtaagggt tcagcttata aagaatggca
     3241 ggcagataac tgcattctgt ccaggagacg gcgcaaccaa gcttatcgac gagcacgatg
     3301 aagtaatggt cgaaagcatc ggcggtgcca aaggaaaatc aaaaggagat attccgggcg
     3361 tcaggtggca ggtcataaag gtaaacgacc aaagccttat tgcactgagg aaaggaataa
     3421 tagagaaggc tagaaaatga gcgaaatact tgcattcaac agatggacta cagagggtat
     3481 ttcagttgca gacgcagggc ttcagagata tatcacactt gagccaaaga ttgtgccaaa
     3541 gacaggcgca agataccatg gaaacaagtt ccataagtca agggtattta tcgtagagcg
     3601 cttgataaac aaggtcatga tcccgggcca taagtcaaaa aagcatgtaa agtcaagttg
     3661 gaagctgaca ggaaaggccc atactgctta tgatattgtt gaagacgcat tcaagattgt
     3721 tgaaaagcag acaaaagaaa acccgctaaa agtctttgtc aaagctcttg aaaacgcagc
     3781 tccaagagag gagataatca ctattgaata cggcggagcc cgctacccaa aagcagtcga
     3841 atgcgctcct caaagaagga tagatatcgc actgcggctg atgacgcaag gcgcttatca
     3901 taaatgtttc aattccaaga gaggaattat agagtgcctt tcagatgagc ttatcaacgc
     3961 ctacaagatg gacagggcaa gcaatgccat ctcaaaaaag ctagagcttg aaaggcagtc
     4021 agacgcttct agataaaatt aacttatttt ttcttcagtt agtgattttc cacccatcca
     4081 cttaagaaca ttcttcttat acctaaacgg ctcatttgca gaaagtgatt catattcttt
     4141 tctaaggcta tctaacgaag cgctctcctt tcccttaagc gtatcaaata tcccaggatt
     4201 tgtcacagca gctcttccaa tcataactcc aacaacaccc attgtttcaa gcttcttaac
     4261 atctgaaaca gtcttaacat ccccattagc gacaatcttt tttccagtcg caacacactc
     4321 aggaaatacg ctccagtcag caggaacatt ataactttcc tttccatgcc ttgcatggac
     4381 gatataatag tcagcattgg tgctattaag aaggtttaag tataccttct tatccttctc
     4441 aaactggttg aggccaagcc gcatcttaac acctacagga tacccccttg attttacaac
     4501 atcgattagt ttatttacct tagagattcg ttttatcata gcagctccga cgccttggtt
     4561 tacaaagttt ggagcagggc acccaagatt caggcaaaat cccttaaagc cttctccagg
     4621 cacaaagctt gaaagaaact tcttcagcca catctcgcgc tgcccgatga cctggataac
     4681 agtaggagct tttgcactaa actcaatcct ctcccaggtg cttttattgt tccgtgcaag
     4741 agattcaaac ctgataagct cagtaaaggc cacatcgcag ccatgcctgc aggcaagtat
     4801 cctaaaagaa aggctggtta tgtcctctat gggtgcaagc atgtattgca tgggatacgt
     4861 attttgctat tgttttataa aacctttggc aatcaaagag ttgctatgca atcgtatagt
     4921 ctctctgtct agccaccttc tcaacccgtt tatatccctg cgggcttaca ataaaaagat
     4981 gaggtgcatc cagagtttca tagcgcaaaa aaaacagcag ccaaagcatc cgcatgtcct
     5041 tccggagttt gagtaagcct ttcctctaat ggttcgccgt caggcaagta gattccacta
     5101 taccatacct tgccaggcat cccagaccat cgaagatgag aaagccgttc tttatcattg
     5161 gttgtatgat gaaatgcata gtggattatt ggggaatcat atccgccctt tctcaattct
     5221 tcctctatcc ttccaggagc atgccccagc ttggcaagat catttaaaga agctattttg
     5281 attatcatat gcttaagcat attttaggca ttgttaaatc ttactagcaa tatttataaa
     5341 tcccccatgc actttggaat taaaatgaaa acattgttta tagaagcaag gtacaaagga
     5401 aaagtagtat tgccaaaaga agtgatagaa aagcttccaa aaaagatagg ccttgccagc
     5461 tctatccaat ttatcggttc cttaaagggt ataagctctc aattgggaaa aggatccctt
     5521 attgcaggcc agatactagg ctgtgatgca aaaagcgcag gaaagattgc agaaagagtc
     5581 gattcttttc tctatatagg cgacggcgaa ttccatccta ttgcaatagc attagagaca
     5641 aaaaagccag tttacaaata caatccaaaa gcaaagactc tcacccagct tgacaaagca
     5701 aagatagatt cctatgaaag aaaaaagaaa ggtgcttacc ttaaatttct caacgcaaaa
     5761 aaagtgggca tccttctcac aacaaaacca ggccaaagga taagtgatgg caagctaaaa
     5821 caattagaac aaaaattcaa ggacaaaaaa ttctacagat tcattgcaga cactatagac
     5881 tatacacagt tggaaaactt cccattcata gaggcatggg taaacacagc ctgtccaaga
     5941 attgaagagg atatccttgc gctaaacatc tcagagttat gaaaccagaa cttctagcgc
     6001 cagtacagga tttcaccagc ctaaaagcag caatagacgc aggtgctgat gcagtctact
     6061 ttgggataaa agaactcaat atgcgccttg gcgcaaacaa ctttacaaaa ggccagataa
     6121 aaaaagtgat agatatctgc cacaaaaaca aggtcaatgc ttattttacg ctaaattcta
     6181 tcatatatga aaatgaaaca agaagaatta atcatcttct ggctattctt aaaaagaata
     6241 agatagatgc gattatcgcc tgggatttct cagtgattag cgagtgtaaa aaactctcac
     6301 ttcctgtcca cctatctacc caggcttcta ttgcaagctt tcaggcagca aaagcaataa
     6361 agaaacagtt cagcaacata aagaggataa acctggcaag agagctgtcg ctgagtcaag
     6421 taagatcaat tattaaacag attaaacagc atcatctaga tatagagatt gaatgcttca
     6481 tccacggcgc gatgtgtgta agcatctcag gccgctgctt tctatctcaa gacatattca
     6541 ataaatccgc aaacagggga gagtgcctcc agccgtgtag aagaaaatac ataatcaaag
     6601 acatagagga agaccatgag tttgaacttg gtagcgatta cataatctct ccaaaagacc
     6661 tttgcaccat aaatatcatt gacaagctca taaaaacaaa tatctcagct ctgaagatag
     6721 aaggaagaaa cagggcgcca gaatatgtaa agacagcagt agagtgctac aggaaagcga
     6781 tagatgcagc aatcaatgga aaacttactc aaagcatcaa agatgagctt gaaaaaaagt
     6841 taagcacagt ctataaccgc ggcttctcta ctggattcta tcttggacag ccggcgaacg
     6901 gatggtcaaa agcatacggc tcaaaagcta caacaaagaa gatcctcatc ggatttgtga
     6961 aaaactatta caaaaagata ggtgtcgctg aagtaaagat agaatcagga aatctaaagc
     7021 ttggtgattc aatcatgttc caaggcccga caacaggagt tgtagagcaa aaaataacct
     7081 caatggaaga ttcaaaagga aagacaaaca aagcagcaaa aggccaattg gtaactataa
     7141 aatcagacca tcttgtaaga gaaaacgaca aactattctt aattaggcct tgtctttctt
     7201 agcaccatat aggcagcaaa cgcaaatgca gagccggata ggataacaaa cctaaaccag
     7261 ttgttcttct taggcggaag ggattcagtg acctggacag tcggctcttg cacagctgct
     7321 gcctggctct cgacagtttt attctcctct gcttttggcg caggcttgat aagcatcttg
     7381 tatagcatct cagctctccc gccatcaatc tttttaaggg tgacagccac atcacccacc
     7441 ttgtcattgt tcacatctat ttccttgccc tgattttctt tcaaaggcac cttgctctta
     7501 ttgtctattg aaagagagat tccatcagcc tgcagcgtta ctacttttat agtatgcttc
     7561 actccgccaa atgtaaacgt tgcttcatcg ccctgggttt gcttgacgct aaagccgctt
     7621 ccgctcacaa aaccttcttt tacatccagg tttgtcttgc tgctggagcc gcctccacca
     7681 ccaccgcctc ctccccctcc accaccgcct cctccgctgc tcgtagtgac agcattggat
     7741 ggcgcagtcg tcgcagtcat gttgataagg tttcctaaag atgagttgtc aagcgtgaga
     7801 ttttcatttc cagcatcatc tattgtcgtg acaaagtaat aatgcacact ggaagagctt
     7861 aatgaatggg tggtagtgca ataaccatca gcacatgagc ttactgtttt tatataatca
     7921 gcagtctcat tgacataccc cttgctcaag ttgacagagg tagctatctt gtaccagatc
     7981 ttatagctca ggctaaaatt atccaggtta ccagcgacgt cttggcttac atagctccaa
     8041 ttaagtgtag ttgtgccaga agatgaagta gcgttaaaca ctcttgggat ctttggcttg
     8101 attgtatcgt ttgccgtacc attaagagaa tatgaaaaca cgttggtatt aaagtttcca
     8161 agtgaatcca ctgtgattag gctataccaa tatgttgttc catgggtagt atcattgtct
     8221 tcataatact gcgttccgtt gggaacagaa gaagcaatca agctaagaga actatttatt
     8281 gcacttatgt tgcttgtatg tctatatatc ctataactct cgcctgtctc gtttccatca
     8341 tcgctccaat tcagctcgat attgccatca gcatcatagt cagccacaga aaagagtgat
     8401 gggcttgtga atggaccagc tattctgatg ctgcttgaat tataccatat gctccagtta
     8461 tcgccgtcaa agaccctcac agaaaatatc caggtatcat tccttgtaag gtttccgctt
     8521 ccaataatag tcgcgttatc aagcgaactt acatacacac tgccattata ccatctggtt
     8581 tcatttaatt gcaatagatc ttcatccaga tcaaaaaagc taaaattggc aacaaggctt
     8641 ccattagtgc ggttcaagat atcagttgaa ttcagtgaaa gattgagaac ttccggaatc
     8701 ctgttgtcaa gcaacaaagc ccattcatca gtaaagttcc agttattatt ggtatcattg
     8761 acataaaact tccatgcgat gtttttcctg ctgttgttag atattgtttt gtttatgctt
     8821 atcaactgta tttttccatc aagggttcca ttcgtggaat ttacccagct tccagtctcg
     8881 ttgatcgaaa agatgtatcc cgacagccca actgtatcat tgacgcttat gttgaactgg
     8941 acagtgccat tgcgctgcgg tctcgttgta ttcgaagaat ttccagacca aacaggtgca
     9001 actgcatcaa gtgaacggac agcttttaag atatctatcc ttgaaaatag gtagccgctt
     9061 ccggatgtat catcgattct ttttccagtg ctgtttaatg ctgcccttat cgatgaaggt
     9121 gatgctgagg taccatcctg cagttttgta aattgtttta acaaagcaac agctcctgcg
     9181 acatgaggtg ttgccatcga agtgccgccc attgctaaga atccatcgct taagtactca
     9241 agcgacatta tgttcaaacc aggcgcaagc agttcaaaaa gagctcctct ttgatattgg
     9301 atggcatcag catcattgac tgcccctatt gcagtcgcat tttgtatgca tgcaggggct
     9361 gaaattccag cagtacatgc aacaccatca caattgcctg ctgcaacagt cacaactatc
     9421 ccttgtgcaa ctgcagcatt tatcgaagca gtctcaggtt catcgttaca ataagtttga
     9481 tatgcctttc catctccaag actcattgag atgaccgata tattgaattt tgttgcatta
     9541 tatgtacacc aatctatacc tgcaataata tcctcgctcc accccgagcc tgcggaattc
     9601 aacgccttta ttgctataat ctttgcagaa ggcgcaacac ccttataggt tgtgttgttg
     9661 gaagcaatcg ttcctgcgac atgcgttcca tggccatgat catcaaaaca agccgca
//
LOCUS       DASRUK010000043         2261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_209174, whole genome shotgun sequence.
ACCESSION   DASRUK010000043 DASRUK010000000
VERSION     DASRUK010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209174"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..940)
                     /gene="lipA"
                     /locus_tag="VFF28_01925"
     CDS             complement(98..940)
                     /gene="lipA"
                     /locus_tag="VFF28_01925"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012960331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HZX44421.1"
                     /translation="MKPSWLKVPIPSGEDYARMRNLTGGSTHTICEEAKCPNLSECWS
                     RKTATFLILGDICTRSCRYCNVKHGTPSKVDAREPHRIAEIIKKLGLKYAVITSVTRD
                     DLKDGGAMTFKRTIEEIRKVSDAKIEVLTPDFKGDISSIKTVLDKQPDVFAHNIETVE
                     RLFPTIRPGASFKQSIIFLKLIKEIDPKQITKSGLMIGLGETKEEILKTMRTLRMVKV
                     DILTIGQYLQPRKDLAEVVKYYTPEEFLELGNLGLDMGFKHVFSGPLVRSSYHAEEAC
                     NKFL"
     gene            1036..1617
                     /locus_tag="VFF28_01930"
     CDS             1036..1617
                     /locus_tag="VFF28_01930"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZX44422.1"
                     /translation="MKGKETEVIRLLIENKANEMSINKIAKLLKKDYKNAHNIVTRLY
                     RMQIVRLQPFGRSYKVIFINKLHPLIFEAEYQRRNDILKNKDIAVMHDSFKVLQSKLF
                     ILLLFGSYAKKTQTKHSDIDLLFVIPDESEANMEKAIQNITGMLPLNIHINIFKESHF
                     KAMINSKEVTVGSEAINSNVILHGIEPYYEMIR"
     gene            1614..2177
                     /locus_tag="VFF28_01935"
     CDS             1614..2177
                     /locus_tag="VFF28_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44423.1"
                     /translation="MIDDRRKKEAGKNFKRYLEEGLLKKEINEPAKSRYLENADLSLR
                     TANELMQSQLKPYLWVIVISYYSMFYTANAVLLHYGYKIQERIAHKVASDALIVLVLD
                     KLRKELIEDYESIQKDALEIASAKAESVIESYSLELDKRSRFQYNMLEVTKESKAHTS
                     LKRAMEFVFEIKFLSRNPHHLWLGMNA"
BASE COUNT          708 a          456 c          458 g          639 t
ORIGIN      
        1 gccttcagca agtccgcaat aaaaaaagaa taatacaaat aaggaccaaa ctatcttttt
       61 cacaaaatcc ccccctgtga gcaggtaatc gctaaatcta taaaaactta ttgcaagctt
      121 cctcagcatg atagctgctt ctcactaatg ggcctgaaaa aacatgttta aatcccatat
      181 caagccccag gttgccaagc tcaaggaatt cttcaggagt gtaatacttg acaacctctg
      241 ccaggtcctt gcgaggctgc aggtattgtc cgatcgtgag tatatccacc ttcaccatcc
      301 taagtgttct cattgttttt agtatctcct cttttgtctc cccaagccct atcatcaatc
      361 ctgatttggt tatctgcttt gggtctatct ctttgatcag ctttaaaaaa atgatcgact
      421 gcttgaaact ggcgcctggc cgtattgttg gaaacaggcg ctcaacagtc tcgatattgt
      481 gcgcaaaaac atcaggctgc ttgtcaagca ctgtctttat cgaacttata tcccctttga
      541 aatcaggtgt aaggacctct atttttgcat cagaaacttt ccttatctct tctatcgttc
      601 tcttaaaagt catcgctccg ccgtctttaa ggtcgtctct tgtgacagaa gtgattactg
      661 catacttaag cccgagtttt tttattatct cagcgatcct atgaggctct cttgcatcga
      721 cctttgatgg agtcccatgc ttgacattgc agtatctgca gcttctcgtg cagatatctc
      781 caaggataag aaaagtcgct gtctttcttg accagcattc agaaagattt gggcactttg
      841 cctcttcaca gattgtgtgc gttgaccctc cggttagatt cctcatccgt gcatagtctt
      901 cgccggaggg gatgggtact ttaagccagg agggtttcat ggctaataag gactggatat
      961 tgttttataa attgtgtgaa aataacaaac tttaaatata tgtaagttta cacataatat
     1021 tatggtgata tttacgtgaa aggtaaggag actgaagtta taaggttgct tattgaaaat
     1081 aaggcaaatg agatgagcat taataagatt gcaaagctcc tgaagaagga ctataaaaat
     1141 gcacataaca tagtgaccag gctttacagg atgcagatag taaggttgca gccatttggc
     1201 aggtcatata aggtaatctt catcaataaa ctccatccct tgatatttga agcagaatac
     1261 cagagaagaa atgacatcct caagaacaag gatattgctg taatgcatga ctcctttaaa
     1321 gtactgcaat caaaattgtt catcctgctg ctttttggaa gctatgccaa gaagacccag
     1381 acaaagcatt ctgacatcga tctgcttttc gtcatccctg atgaatcaga ggctaatatg
     1441 gaaaaagcaa tacaaaatat cacaggaatg ctgcctctaa atattcatat caatatattc
     1501 aaggaatccc acttcaaggc aatgataaac tcaaaggagg ttacagtggg atcagaagca
     1561 ataaacagta atgtgattct tcacggtata gaaccctatt acgagatgat acgatgatag
     1621 atgacagaag gaaaaaggaa gctgggaaga atttcaagag atatcttgag gaaggccttt
     1681 tgaagaagga aataaacgaa cctgcaaaga gcagatatct tgagaatgca gatctttcgc
     1741 ttaggacagc aaatgagctt atgcaaagtc agttaaagcc ttatttgtgg gtcatcgtca
     1801 tatcatatta ttctatgttt tacacggcaa atgcagtcct gctccactat ggttataaaa
     1861 tccaggagag gatagcccac aaggtagcaa gcgacgctct aatcgtcctt gtgctcgata
     1921 agctcagaaa ggaactcata gaagattatg aatctataca gaaagatgcc ctggaaatag
     1981 caagcgcaaa ggcagagtcg gttatagaat cttactctct tgagctggac aaacgctcaa
     2041 gattccaata taacatgctt gaagtaacaa aagaaagcaa ggcccatacg tctctaaagc
     2101 gagcaatgga gtttgtattc gaaattaaat ttcttagcag aaatccccac cacttgtggt
     2161 tggggatgaa tgcgtaacac ttataggatg gctaattctg tgattatctg ttggtaaaat
     2221 gatgagcctc aaaaaagagg tgaggcccaa tgacacttta c
//
LOCUS       DASRUK010000044        18933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_878260, whole genome shotgun sequence.
ACCESSION   DASRUK010000044 DASRUK010000000
VERSION     DASRUK010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 18933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18933
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878260"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..571
                     /locus_tag="VFF28_01940"
     CDS             209..571
                     /locus_tag="VFF28_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44424.1"
                     /translation="MQSDELAPNPREVITNMLDGYWHQVRQAAVRLGLKPQREELFAA
                     CGLIVGKTSGNYADVLDQCRGHMPTTQAEIYLAQLSRYFRSLDIIGPDLEGSTKLVDQ
                     AYECYSRLVQILGAENPE"
     gene            771..1322
                     /locus_tag="VFF28_01945"
     CDS             771..1322
                     /locus_tag="VFF28_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44425.1"
                     /translation="MDRDEAIQKFLIEVYGYGKIQEFIHEGKHEKGVTIRQFLDETIV
                     GTFRGEAVSEKSPNVEIIVRWPRISTIKPVTDAQTRLTRTDLSYNIYNQSYFEGMSFD
                     QTGTFLMERVMPLFPGVQLTHDKRTSWWVYKIDTSSFRLYLQGSYAHHSKQLSTDFYA
                     PSELVNTLNTTVNQYYTIMRALK"
     gene            complement(2238..2918)
                     /locus_tag="VFF28_01950"
     CDS             complement(2238..2918)
                     /locus_tag="VFF28_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44426.1"
                     /translation="MADQKVVKVKKKRWVEVVSPQLFRNEIIGEIPVFEPRTLEGKLM
                     TVNLMTLTKDMKRQNASIKFLLTSVKGDKASTETYGYYLSPTSMRRMVRRGKEKIGFS
                     AVYMTADNKRIRIMPVIIPVTKVKGSVAAAYRKHATNFFVTYASKVSFDEMIRDLITT
                     KLQKELKNELKKIYPVKILEVAKLHIENKKVPVEVSKAVEPQPEEEAEEQIEPAKEET
                     AEPVEEQQ"
     gene            complement(2961..6149)
                     /gene="gyrB"
                     /locus_tag="VFF28_01955"
     CDS             complement(2961..6149)
                     /gene="gyrB"
                     /locus_tag="VFF28_01955"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014052056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HZX44427.1"
                     /translation="MPENNQSEYTAKDIQVLGGLSAVRKRPSMYIGDTAITGLHHIVF
                     EVVDNSIDEAMAGYCKNISVIIHKNGMVSVIDDGRGIPVDIMPKFNKSAVEVILTVLH
                     AGGKFDKTAYKVSGGLHGVGVSCTNALSKELIVQVKRNNKLYEQKYERGNPMTPLLEV
                     GTSTNTGTTVTFLPDQEIFPDIIFHYDILANRLRELAFLNKGIRITVEDERTAKKDEF
                     HYEGGIISFVEYINKNKAPYHDVIYFHKEREKLEIEIAMQYNDTYQENVYSFANNINT
                     VEGGTHLSGFKTALTRAINSYIEKNKLSDIKLTSEDVREGLTAVISVKIAEPQFEGQT
                     KAKLGNSEVKGIVDSIINTELSHFLEENKQVARSIIDKSINAARAREAARKAKELTRR
                     KGALGHSSLPGKLADCSNRDASKTEIYIVEGDSAGGCFSSDTKVALADGRNLNFTELI
                     AEDKQGKKNYCYTIKEDGSIGIGLIKNPRKTKESAEVIKIRLDNEEEIFCTPDHRFMQ
                     RDYNYLRADKISPQDSLMPLNRKLSKLEGRITIQGYEMVYDLKSDRWVFTHILADRYN
                     QENNICLPSVGEYKHHIDFNRLNNNPDNIIRMTKKEHLSLHALMVEKTLFRPDVKEKS
                     RLAHTTPEYKEKIRRIMSAPQMKSILSKRAKKQWEDGEYREFMLNKFLEFYRENEEYR
                     LKSQEILKKGQMKYWANEENRKLHSQKTHNYFLNNPDKKTQLSSASKKQWQDTELKKW
                     RSDKTKEQWTEEFRAKRKKAYDATYFENTIKCMKSLFEQDGNLEAYDKERIRSKNRNL
                     LKKETFTERFFGSDEGRMLEAVENYNHKIINSMPVTQRMDVYDLEVEGTHNFALASGV
                     FVHNSAKQGRNREFQAILPLRGKILNVEKARIDKMFANEEITALITAIGTSIMEEFDL
                     GKARYHKIIIMTDADVDGAHIRTLLLTFFYRHMVALIEAGYIYIAQPPLFRVKKGKEE
                     IYVYNDEKLKQVLAEKGEEVTIQRYKGLGEMNPQQLWETTMDPSRRVLLRVTLEDAVK
                     ADRIFSILMGDQVEPRRKFIEEHAKEVMNLDV"
     gene            6398..7252
                     /locus_tag="VFF28_01960"
     CDS             6398..7252
                     /locus_tag="VFF28_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 family protein"
                     /protein_id="HZX44428.1"
                     /translation="MDRKLHEQLLAAQKNEITEHLVYKKLSGQDAKNRKTLKRISDDE
                     LSHYKFFLKHTGKEVKPSTFNLWKYYLMASIFGLTFAVKLMERGEKRAQINYSELAIK
                     GIKDIEKDEEEHEKKLIGLLDEERLKYVSSIVLGLNDALVELTGSLAGFTLALANSSL
                     IAMTGLIVGIAAAMSMAASEYLSVKSGDSTKTPFKSSIYTGIAYIFTVLLLILPFLLL
                     DNVFIALGLTFTTVVVIILFFTYYISVVNEVSFSKRFLEMAAISIGVALLSFGIGYLV
                     KVLFGISV"
     gene            7359..8513
                     /locus_tag="VFF28_01965"
     CDS             7359..8513
                     /locus_tag="VFF28_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44429.1"
                     /translation="MVVSKELSTGTIISPLFAHGGDAVARDHNLVSYHYAGDIGLPRL
                     QKKVINPFEGTLEDITYREAAKKVGADSDGVLNERSRGRQLRLGGLVFRPQSRPTTNF
                     EYEGIFTDMMSLLHGRYEKGPAIIKIGTHSFIQLDDFMRAMSLIKADHQYKSNTTTLR
                     ARDHRTGEEVGPDPRIAEMAVAFDDSRYRQITPDAASQYKQALTLRANMRDFRRRVLL
                     RELGSYGIAHRGLAGQVSHPVKLSDGSAAELVFAPQAHDEYNGTADRRDRKVYSGLFA
                     HRRRDLSAHTGDLVITSSLAYHPPYQKFEDGKGLEVRTTKGEKTDHIHIMRIDGRKPL
                     KYTLLKREGQVYISVEDIESAFLSLQARYSIMPTGLRVDLYPARPAHAYL"
     gene            complement(8504..8632)
                     /locus_tag="VFF28_01970"
                     /pseudo
     CDS             complement(8504..8632)
                     /locus_tag="VFF28_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013103179.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(8607..8936)
                     /locus_tag="VFF28_01975"
     CDS             complement(8607..8936)
                     /locus_tag="VFF28_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44430.1"
                     /translation="MKTKKPLLLSIVSLLVTPLTLADDCGITNLAQCIPQKLFEYLLS
                     LINAPFQPFLALTKSLLYEAVNVSVFQPLWAIIVYIISIIKNHQSSLFNRYRLVEREI
                     LRKASAS"
     gene            complement(9175..9759)
                     /locus_tag="VFF28_01980"
     CDS             complement(9175..9759)
                     /locus_tag="VFF28_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44431.1"
                     /translation="MRHLIEQPTDVASGKRIYTAIATYVSDRVEVNISDGTRQLDAIL
                     LSGGSDGTTELDHLLYLSRHDGYLQGDILYVVREGRGPRLVIGFSEDMQATVEGQPLV
                     SSVSEYKEGGIIRAVPVLHTVWEHQNGTEYTGIAWVPRYNRNGEESFFQKVRIKGCIG
                     IAPGDLVMEAGITHIGKNITAMTSKNYVISPSGR"
     gene            complement(9909..10550)
                     /locus_tag="VFF28_01985"
     CDS             complement(9909..10550)
                     /locus_tag="VFF28_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44432.1"
                     /translation="MKMTFLFAVTVMLTGIVAGEDYQTVEGSSTQDETILTGARGMYV
                     DKSDDGYECYKRDGRTICQDSDDNICYRGIERWHCEFDDDYLYDCEKTGHGWRCFNDS
                     SSKRVITRDDYCRSDGCTRYAGYKDSTDYGNYGNDNLFRSRDDYGYYTYKEDGRDTRK
                     SSTDKHFRRDYKADYDYIDRWYCSKEYGRWYCGGLEDDDDFSGSRVLSKMIKP"
     gene            complement(10641..11528)
                     /locus_tag="VFF28_01990"
     CDS             complement(10641..11528)
                     /locus_tag="VFF28_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44433.1"
                     /translation="MKRLFVSLFLFLFALASMGYANDMVIANSHISAENGGVNYITFV
                     VIDNKDGSTEMDHYGDFESDDLHAYDESLGIMYEENDCENMGGCKDVSYEGFCDEDGN
                     CDEDEPGYDEEEGAYAEDEEYDSCDYSLCYGDDCIEDDDGSYWCDGEDCDWFCIDEET
                     SDCEDGSCEEDEEEFDCNEDGSCDEDEEEFDCSDGSCDEDEFDCSDGSCDEDEEFDCS
                     EDGTCEEDEEDCQDGSCDDEFDCQDGSCEDEYEDADMFCDDDGNCYEELEDNDYKEEE
                     VSSSDDEWVSRIIGGLIDI"
     gene            11661..11804
                     /locus_tag="VFF28_01995"
     CDS             11661..11804
                     /locus_tag="VFF28_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44434.1"
                     /translation="MKVTCEDGFAIQIKNEKEIIEHTQTHMKNFHNKDMSADDIRGIM
                     KAA"
     gene            11804..11998
                     /locus_tag="VFF28_02000"
     CDS             11804..11998
                     /locus_tag="VFF28_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HZX44435.1"
                     /translation="MEILKVTCEDGFEVRSVNEKEVIEIIKGHMKKFHGVDLTAEDVI
                     EMIEVVHHHSVDHPAFFLSE"
     gene            12263..13129
                     /locus_tag="VFF28_02005"
     CDS             12263..13129
                     /locus_tag="VFF28_02005"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HZX44436.1"
                     /translation="MTNEVGGILRQLGIKPAYVKRFKKGAINKTYDIQAQQNYVLRIY
                     PRDFWKAKKEAYLYRLLGKKTDVPVPNVIKQGRNYLLMQKVEGKALSLSDRKLVKASG
                     EMLAKIHSVKFSSFGWIIGRQIEPKFTTWEKFVKYDTEKKLGKIPGHLFLKREIKKII
                     FDNNHLLKIAEKPCLLHKDYHSSHILTHDGRINGIIDMEWAMSGHNELDLAKTFIWMF
                     NGRPSLQNIFMKGYLKHGTVSKQFYERLALYRILTLLSAVSFSYECGNKKWCKVNLKQ
                     LKGELNEYHKTH"
     gene            13107..13739
                     /locus_tag="VFF28_02010"
     CDS             13107..13739
                     /locus_tag="VFF28_02010"
                     /inference="COORDINATES: protein motif:HMM:NF025210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HZX44437.1"
                     /translation="MNITKLTEEYINEHPSIKDCVRKGLMNYSSLTRKIVEDENLSLK
                     DNFDAILIACRRYYAKIKKEEILENKIMDLLKKSKLEVKNKIVVVVVEKDVYFNHLIE
                     LHKDVKKKAGVFHIIEGSSTITIITSSEFLDEISKLFRNKIIRVSENLAEVNLKSPEE
                     LEKTPGVIAYLSTLLAENGINIIETMSTWTDTLFVISEEDIAKVMGVLRF"
     gene            14030..15004
                     /locus_tag="VFF28_02015"
     CDS             14030..15004
                     /locus_tag="VFF28_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44438.1"
                     /translation="MADLKPLVIFISACSAYAGLALNLSKKWKQEIGSKVVLGGRHFE
                     EPRLSHYSPQELTLDSQVRQPITTSLLWGQQNKCLDYVYSGSGGNIPHLLKAIEDGLT
                     ADAEGIFTLSGRYLQGKGRCREFPAGIIPISKAKFEDGDDYLLRVIVSDYCPEGCGFC
                     VGPSRLGVSFDEALLSQAATAIIANTCDKTTIIQFCDSNPIRPDSIDKYGQIIKQVRK
                     AKPYRQYGMFMNTAYLIDRRFFDYTMQFVRTMDIMSLFVGRDCLTGEIAGAIGRNHNR
                     LSRSQEMLDGEQNVICKILSASDQYQKFNDFIHTCTSSDNKRIYSKDG"
     gene            14997..15359
                     /locus_tag="VFF28_02020"
     CDS             14997..15359
                     /locus_tag="VFF28_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44439.1"
                     /translation="MAEEMDMFARTAVNSGVNLTIQLGVLQPYPGTPLANSHMRYDDK
                     IEHFIDGNHTQFWLSNDSALANILTTVRIMGELKSQYEGTVKFIFDLHSKNERVLYET
                     PSMREVMQKSSGLYLYRK"
     gene            complement(15378..15965)
                     /gene="ribA"
                     /locus_tag="VFF28_02025"
                     /pseudo
     CDS             complement(15378..15965)
                     /gene="ribA"
                     /locus_tag="VFF28_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635942.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
     gene            complement(16000..16167)
                     /locus_tag="VFF28_02030"
     CDS             complement(16000..16167)
                     /locus_tag="VFF28_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44440.1"
                     /translation="MEKEKIYEDDEEESGLYEEYGKEDLGSDDEVSPEEEGFMQGYDE
                     VDEEPSDEDIS"
     gene            complement(16235..16828)
                     /locus_tag="VFF28_02035"
     CDS             complement(16235..16828)
                     /locus_tag="VFF28_02035"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HZX44441.1"
                     /translation="MSMINFMLIMQIFILLNPLASVPFLISAYKRRLNVRIIAFKSVI
                     GAFIIAFIIAISGHLLFDIFGITLNGLKIAGGVVLILLGINMIRPKNGGHEVGDIDGL
                     AALIATPMLTGPGTISFITVKVYEIGRLDIIFNLILAFLLLGIVFITLAMFINKINLK
                     IIDVLSRVFGLFLTAVAVEMIAKGISGFVQAYAILPK"
     gene            complement(16875..17096)
                     /locus_tag="VFF28_02040"
     CDS             complement(16875..17096)
                     /locus_tag="VFF28_02040"
                     /inference="COORDINATES: protein motif:HMM:NF014100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF131 domain-containing protein"
                     /protein_id="HZX44442.1"
                     /translation="MKHLITVGIVVLFIGLALILLGILSSGKSKIAVGGFIGFIPFGF
                     ATDRRLLWIGVVLAVLMLIFYLIIFLYPR"
     gene            17266..17562
                     /locus_tag="VFF28_02045"
     CDS             17266..17562
                     /locus_tag="VFF28_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44443.1"
                     /translation="MRHKEGTYLYASLITKKMDAETKSFIENVDSWIKQIRSEFTQIT
                     DVSTIVEENVNNTQHNYELIYELKEEIEQLKSEIKTLKIMQLMELKEKLARPVQ"
     gene            complement(17546..17842)
                     /locus_tag="VFF28_02050"
     CDS             complement(17546..17842)
                     /locus_tag="VFF28_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44444.1"
                     /translation="MAQEDLVSLGGNIELVGFSKVESLRQIVLKKIIGNYVRQIQDKR
                     KDYEKLSITLLEQDNDCKVKVELKLGANILVSESVDCNVFMAADSALKKIIAQA"
     gene            18065..>18933
                     /locus_tag="VFF28_02055"
     CDS             18065..>18933
                     /locus_tag="VFF28_02055"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44445.1"
                     /translation="MLVESFSGIRGIYGLDLTSDVAGRYAFAYYNYLSQKIGRGPIIV
                     VGRDTRPSGSAVYKAVIESVTNVLDVGIIPIPALSLAVREFKADGGIMITASHNEPEF
                     NGLKFLSSDGAVLVPNEMQTVINSFRKVKDVPARKPSIKIKDISSVYSKFIGNLTGTI
                     ENNYKVIADANGGTGYLLEKVAKDLGVKNIRVINQEQGIFKRKVEPTKESLAYLRDMI
                     KADGAVFAAGFDCDADRVEILLDDGTIVDGNMLLAIIVDHMVGNKKGVVVVNDATSNI
                     IRDICAKHGCLLK"
BASE COUNT         5500 a         3875 c         3713 g         5845 t
ORIGIN      
        1 gaaccgctaa acggctgtta accgagtcat gccctacgag caaacatata caaaacatag
       61 cgactgacaa aggagcgttc ttttgtcaat gtatcttaaa gaaattagtc tatatgtttg
      121 cggagtgggg ttgaccgagc gcaagcgaag agttatgacg caggggtttt ctgcaaataa
      181 ttaaaaagaa gacggtattc gtatctgtat gcagtcagac gaattagcac caaatcctag
      241 agaagtaatt actaatatgc ttgatggtta ctggcatcaa gtaaggcaag ctgccgtacg
      301 acttggatta aaacctcaaa gggaagaact ttttgcggct tgtggtttaa ttgttggcaa
      361 aacttcaggt aactatgcag atgtacttga tcaatgcagg gggcatatgc caacaacaca
      421 agcggagata tatttggcgc aattaagcag atattttcga tcactcgata ttatcggtcc
      481 tgatcttgag ggttcaacta agttagttga ccaggcttat gagtgttatt ctagattggt
      541 acagattctt ggagcagaaa accccgagta ataattttgg ttaacaaagg ctcgttaaac
      601 tctccaaccc tacagagtgg ggttggattt cgaccgaaaa gtttttaaaa atccttatta
      661 aagggcgaag cccattagag ttaatacgtc aagggaaggg gaactttgaa tcttagaggt
      721 gattataaac ccgaaagggt tttatattat ccttaattcc tatttttaat atggataggg
      781 atgaagcaat tcagaaattt ttgattgaag tttatggata tgggaagatt caggaattta
      841 tacacgaagg taaacatgaa aagggggtaa caatacgcca attcttggac gaaactatag
      901 taggaacgtt tagaggagaa gctgtaagcg agaaatcgcc caatgtagaa ataattgtgc
      961 gatggccaag aatttcaact ataaaacccg ttactgatgc tcaaactaga ttaacacgaa
     1021 ctgatttatc ctacaatata tacaaccaat catacttcga aggaatgagc tttgaccaaa
     1081 ctggcacatt tttaatggaa agagtgatgc ccttatttcc cggtgtacaa ttaactcacg
     1141 acaaaaggac gagttggtgg gtttataaaa ttgatacttc ttctttccgt ctatatttgc
     1201 aaggtagtta tgctcaccac agtaagcaat taagcaccga tttctacgct ccctcagaac
     1261 ttgttaacac cttaaataca actgttaatc aatattatac tataatgcgt gctttgaagt
     1321 agccagaaaa gttccccttc ttgaaaaaag gatttttgaa aattgagttt aacaagcgtt
     1381 aaccgcaatt gttgtgggca tattagcggc tattaaaaga aaaatagccc cgggcaggtt
     1441 caccaccagc ctttttgccc ttcaacaaaa aagcatgggg aaatggggaa tttaccttcg
     1501 ctaaattcaa gcgtttgttg caataaaata gcagaatgaa aaaatagccc cgggcagatt
     1561 cccacctcag aacctaggtt ccgagacctc cttggcaatc aggcgtttat ttcattgaat
     1621 ggttagaaaa aatagccccg ggcaggttcc cacctcagaa cctaggttcc gagacctcct
     1681 gggtaatgct gtcctttaca attaatggta taaaaatata gccccgggca gattcgaact
     1741 gccgtcagag cctttctttg gcaggaattc ctgcctccag agggccctat ccttaatctt
     1801 ctttgacttt cgtccaagaa tttgaccgct agattccgca gaacagtatc acctgttctt
     1861 ctacggggct atgcggtcaa taacacaaga gattgcgttt tatatttaaa attaaccctt
     1921 gggggatttg taatatcttt tttaagccag agcttgacct tgtttgggtt actgggattt
     1981 atcaagcgga acaacgtttg cctttcatag gtaatgatcg tattttttgc ccttttatct
     2041 ttcatcatct aaactgccag ttatcgttat tctattatac gacttccatc gccacaaagt
     2101 tcggcttgat taatcctttt cattcaactt ccagtgtaat tgatgtccgc tgaaatcatc
     2161 attcagcagt cgaatcttta tgtgatgcaa atatcgaaaa aacaaagaat gatcgattaa
     2221 tgaataacaa aaaatgatta ctgctgttct tcgacaggct ctgcagtctc ttcctttgca
     2281 ggctcaatct gctcttcagc ctcttcttct ggctgaggtt caactgcttt gcttacttcg
     2341 actggaactt ttttattctc aatgtgaagt tttgccactt ccaggatctt tactggatat
     2401 atctttttaa gctcattctt aagctctttt tgaagcttgg ttgtgataag gtctcttatc
     2461 atctcgtcaa aacttacttt tgaagcatag gtgacaaaga aattagttgc atgctttctg
     2521 tatgcagctg caacagagcc tttaacttta gtgactggga ttattactgg cattatcctt
     2581 atccttttgt tgtcagcagt catatataca gctgaaaatc ctatcttttc tttgcctctt
     2641 cttaccatcc ttctcatgga tgtagggctt aaataatatc cataggtctc ggtcgatgct
     2701 ttatctcctt tgacgcttgt aagaaggaat ttgatgctag cattctgcct tttcatatct
     2761 tttgtcaggg tcatcaagtt tacagtcata agctttcctt caagggttct tggctcaaat
     2821 actggtatct ctccgatgat ctcgttcctg aaaagctgcg ggctaactac ctcgacccat
     2881 ctttttttct tgacttttac tactttttga tctgccattc tgatgttgag aaaaggagtt
     2941 gatttataaa gcttcttatt ttatacatca aggttcatga cttcctttgc atgctcttcg
     3001 ataaacttcc ttcttggctc cacctgatcg cccataagta tcgaaaaaat ccggtcagct
     3061 ttaactgcat cttcaagggt tacccttagg aggactcgtc tggaagggtc catagtcgtc
     3121 tcccatagct gctgtggatt catctctcca aggcctttat atcgctggat ggtcacttct
     3181 tcgccttttt ctgcaaggac ctgcttgagt ttttcatcat tatagacgta tatctcttct
     3241 tttcctttct ttaccctaaa gagcggaggc tgggctatgt atatgtatcc ggcttctatc
     3301 aatgcgacca tgtgcctgta gaagaatgtc aaaagaagtg tcctgatgtg ggcgccgtca
     3361 acgtcagcgt ctgtcatgat tatgatctta tggtatcttg cttttcccag gtcaaactct
     3421 tccatgatgc ttgtgcctat cgctgttatc agcgcagtta tctcttcatt tgcaaacatc
     3481 ttgtctatcc ttgctttttc tacattaagg atctttcccc ttaatggaag tattgcttga
     3541 aattccctgt tcctgccttg ctttgctgag ttatggacaa aaactcctga tgcaagtgca
     3601 aagttatgtg ttccttctac ctccaggtca tatacatcca tcctttgcgt aacaggcata
     3661 gagtttatta tcttatgatt atagttttca accgcctcga gcatccgacc ttcatcagat
     3721 ccaaaaaagc gctcggtgaa tgtttctttc tttagcaaat tcctattctt agacctaatc
     3781 ctctctttat cgtatgcttc taaattacca tcctgttcaa acagagattt catacacttg
     3841 atcgtgtttt caaaataagt ggcatcataa gctttttttc ttttggccct aaattcctca
     3901 gtccattgct cttttgtctt atcgcttctc cattttttta gctcggtatc ctgccattgc
     3961 ttttttgatg cactacttag ctgcgtcttt ttatcaggat tatttaaaaa gtaattgtgc
     4021 gttttttggg agtgcaattt tctattctct tcatttgccc aatatttcat ttgacctttc
     4081 tttagtattt cttgagattt taatctatac tcttcatttt ctctataaaa ctccaagaac
     4141 ttgttcagca tgaattccct gtactctcca tcttcccact gtttttttgc tcgtttagac
     4201 aggatgctct tcatttgagg ggcagacata atccttctta tcttttcttt atactcagga
     4261 gtggtatgag ctagcctgct tttttcttta acatcaggcc taaacaaggt cttctcaacc
     4321 atcagggcat gtaaagatag gtgctctttt ttagtcatcc taataatatt atccggatta
     4381 ttattcaaac ggttaaaatc aatatggtgt ttatactcac caacagacgg aaggcagatg
     4441 ttattttcct ggttgtacct atcagcaaga atatgggtga aaacccacct atcgctcttt
     4501 aaatcataaa ccatctcata gccttggata gtgatcctcc cttcaagttt agatagtttc
     4561 ctgttcaaag gcatcaaact gtcctgagga gatattttat ctgcccttag gtaattataa
     4621 tccctttgca taaatctgtg gtcaggagtg caaaaaatct cttcttcatt gtcaaggcgg
     4681 attttgataa cctctgcact ctctttagtt ttccgagggt tttttatcag gccaatccct
     4741 atagagccat cttccttgat agtataacaa taatttttct tgccctgttt gtcctcagca
     4801 ataagttcag tgaaattaag gtttcttccg tctgcaagtg caacctttgt atcagaagaa
     4861 aaacagccgc ctgcagaatc tccttccact atgtaaatct cggtctttga agcgtcccta
     4921 ttgctgcagt cagcaagttt tcctggaagg gatgaatggc caagagctcc ttttctcctt
     4981 gtaagctctt ttgcttttct tgctgcttct cttgctcttg ctgcattgat tgacttgtct
     5041 atgatcgacc gtgcaacctg cttattttcc tcaaggaaat ggctaagctc tgtgtttatg
     5101 atagaatcaa caattccttt gacttcggaa tttccaagct ttgctttggt ctggccttca
     5161 aattgaggct ctgctatctt tactgagata acggcagtca agccctccct gacatcttct
     5221 gaagtcagct ttatgtcgct aagcttgttt ttctcgatgt agctgtttat cgccctggtc
     5281 agtgctgtct taaatcctga aaggtgggtt cctccttcaa cagtgtttat gttatttgca
     5341 aaggaataaa cattttcctg gtaagtgtca ttatactgca tggctatctc tatctccaat
     5401 ttttctcttt ccttgtggaa atagatcaca tcatggtaag gggccttgtt cttgtttata
     5461 tactctacaa agcttatgat tccaccctca tagtggaatt cgtctttttt tgctgtcctt
     5521 tcatcctcta cagttattct tatgcctttg tttaggaaag caagctctct tagcctgttt
     5581 gcaagtatgt cataatggaa aatgatgtct gggaaaatct cctggtcagg aaggaatgtt
     5641 acagttgttc ctgtgttggt gcttgtgccg acctccaata ggggggtcat cggatttcca
     5701 cgctcgtatt tttgctcgta aagcttgtta tttcttttga cctggacgat aagctcttta
     5761 gaaagcgcat tggtgcagga gacgccaact ccgtgaaggc ctccgctcac tttatatgcg
     5821 gtcttgtcaa actttcctcc tgcatgaagc actgtcagga tgacttcaac tgcagacttg
     5881 ttaaacttag gcatgatgtc aacaggaatt cccctgccgt catcaatgac tgaaaccatg
     5941 ccgttcttgt ggataatgac gctgatattc ttgcagtagc ctgccatagc ctcgtcaatc
     6001 gagttgtcaa caacttcaaa gacaatatgg tggaggccgg ttatcgctgt gtcacctata
     6061 tacatggacg gcctttttct tacagcactt aggccgccaa gtacctggat atcttttgca
     6121 gtatattctg attggttgtt ttctggcatt aatttcccct agaaactttt ttcctggtta
     6181 atttcaattg gggcttgttt ataaaggttt ctattaaatt tggggtttat agcgtctata
     6241 aaggctgaat ttaagggttt tggatggacg agaagagtaa aatgttatga gtgagccatg
     6301 atgagtaata ttatgcaaaa caaccattta tcataacata tccaatttat gtcgaggact
     6361 aggaactttt aaatagtctc aaaccagaaa acagcctatg gacaggaaat tacatgagca
     6421 acttcttgcg gcgcagaaga atgagataac cgagcatctt gtctataaaa agctcagcgg
     6481 gcaggatgca aaaaatagga aaacattaaa gcgcatatct gatgatgagt taagccatta
     6541 caagttcttt ctaaagcata ccggaaagga agtaaaaccc tctactttca acctttggaa
     6601 atattatctg atggcttcta tctttggcct tacctttgca gttaaactta tggagagggg
     6661 agaaaagcgc gctcagataa attattctga actggctatc aaaggcataa aagatattga
     6721 gaaagatgaa gaagagcatg agaaaaaatt gatcggactg cttgatgaag agcgcctaaa
     6781 atatgtgagt tctatagttc ttggacttaa tgatgcacta gtagagctta ccggcagcct
     6841 tgcaggattt actcttgcgc ttgccaactc ctcacttatc gccatgactg ggcttatcgt
     6901 cgggattgct gctgcgatgt caatggctgc atcagaatat ctttctgtaa aatcaggaga
     6961 ctcgaccaaa acacccttca aatcttcgat ttacacagga atcgcttata tatttacagt
     7021 actgcttttg atactacctt tcttgcttct ggataatgtc tttatcgcct tgggacttac
     7081 gtttactacg gttgttgtca taatcttatt ttttacgtat tacatctctg ttgtcaatga
     7141 ggtctctttt tcaaaaagat ttcttgagat ggcagcaata agcataggag tagccctgct
     7201 tagctttggc atcggttatc tagtcaaagt gttatttggg ataagtgttt aagtcagata
     7261 ttaaaaaccc aaagtaggcg ctttattggt gcaatatgct taattgacgc ttgtcctgca
     7321 agctaaggta taaaaaccta gaaatcaatc taccaatcat ggttgtttct aaagaattat
     7381 ccaccggaac catcatttct ccgctttttg ctcatggggg agatgctgtt gcacgagatc
     7441 ataatctagt cagctatcac tatgcagggg atatcggcct tccaaggctg caaaaaaaag
     7501 tgatcaatcc ttttgaaggc actcttgaag atataaccta tagagaagca gctaagaaag
     7561 tcggcgcaga tagtgatggt gtactaaatg aaagatctag gggcaggcag cttaggcttg
     7621 gagggcttgt ctttcgacct caatcaaggc ctactactaa ctttgaatat gagggtatat
     7681 ttacagacat gatgagctta cttcatggaa gatatgaaaa aggccctgca atcataaaaa
     7741 ttggcactca tagctttatc cagcttgatg attttatgag ggcaatgtcc cttataaagg
     7801 cagatcacca atacaaatca aataccacta cccttagggc aagagaccac aggacaggtg
     7861 aggaggtagg gcctgatcct agaattgctg agatggctgt agcattcgat gactcaaggt
     7921 acaggcagat aactcctgat gcagcaagtc aatataaaca agcacttacc ttgcgggcta
     7981 acatgcgtga tttcaggcgt agagttcttt taagggagct gggatcttat ggtattgccc
     8041 atcggggctt agcagggcag gtgtctcatc cagtcaaatt atccgacggg tctgctgcgg
     8101 agcttgtttt tgcgccgcaa gcacacgatg aatataatgg cactgctgac aggcgggaca
     8161 gaaaagtata ttcaggactt tttgcacata ggagaagaga tttatcagcg catacaggag
     8221 acttagtcat cacgagcagc ctagcctatc acccacctta tcaaaagttt gaagatggca
     8281 aggggttaga agtcagaaca acaaagggag aaaagacaga ccatatacac atcatgagga
     8341 ttgacggaag aaaacccctc aagtacaccc ttctaaaaag ggaggggcag gtctatataa
     8401 gcgtggaaga tattgaaagc gcatttttat cgcttcaggc cagatattca ataatgccaa
     8461 ccggactaag ggttgattta tatccagcaa ggccagccca tgcttatcta taacttattt
     8521 cttgtttaaa cctatccaga aattcctgca tgaattcatg cctctcttca gccatctctc
     8581 ttgcagtctc tgtgttcatc ctatccttaa gaagcagaag cttttcgtag aatttctctt
     8641 tccaccagtc tatagcggtt aaagagcgat gactggtggt tcttgattat tgagatgatg
     8701 tagactataa tggcccacaa aggctgaaaa actgagacgt taactgcttc atacagtaag
     8761 ctctttgtaa gagcaaggaa aggctggaaa ggagcattga tcaagctcag gagatactca
     8821 aatagcttct gagggataca ttgtgctaga ttggttattc cgcagtcatc tgctaaggtt
     8881 agaggtgtaa ctagcaagga aactatagac aacaataatg gttttttggt tttcatggtt
     8941 tttacctcca aagatttata taacggataa ttagattaag agcttagatt gttcaaggca
     9001 tattgcttgg tttgtccata acagcaccaa ttggtccaac caacttgaaa atagaaggga
     9061 ttgaaagaag tttggaaatc tgcaattatc tttaatttag gtattattac tgcattgatt
     9121 tattatttat ttttgcaaaa acctgatttg aattgcggat ttgatagcca ctcgctatct
     9181 tccagaagga gagattacat aattttttga agtcatagcc gtaatattct tacctatatg
     9241 cgtaattcct gcttccataa ctaaatctcc cggggctata ccaatacatc cttttattcg
     9301 gactttttgg aaaaaagact cttcaccatt ccgattgtat cttggaaccc atgcgattcc
     9361 agtatattct gttccatttt gatgttccca gacagtatgt aaaactggaa cagccctgat
     9421 tatccctcct tctttatact cacttacaga gctaactagc ggttgacctt caactgtcgc
     9481 ttgcatatcc tctgaaaaac cgataactaa tctggggcct cttccttctc ggacaacgta
     9541 tagtatatcc ccttgtaaat atccatcatg tctactaaga tataaaagat gatctagctc
     9601 agtagttccg tccgaacctc cgctcaataa aattgcatct agttgtcttg tcccatccga
     9661 tatatttact tctactctgt cacttacata tgttgcaata gctgtatata tgcgcttgcc
     9721 acttgcaaca tcagttggct gctcaatcaa atgtctcata aatcaaggga atcttatctg
     9781 gctttataaa ttttatttgt tgtaaatacc ccactcactt gcggtgactg gtggtttttg
     9841 acaactcggc agttttggcg atagtcttta tccatagatg gatgcaaata atatagaaat
     9901 aaaaaaggtt acggttttat catcttggac agtactcttg aaccactgaa atcatcgtcg
     9961 tcctccaagc cgccacaata ccagcggcca tattccttgc tgcaatacca tctgtctata
    10021 tagtcataat ctgccttgta atctcttcta aagtgtttat cggtcgaaga ttttctggtg
    10081 tccctgccat cttctttgta tgtataatag ccataatcat ctctggacct gaataaattg
    10141 tcattcccat aattgccata atctgtgctg tctttatacc ctgcgtatcg ggtacaacca
    10201 tcacttctgc aataatcatc cctggtaatt accctcttag aactactatc gttaaagcag
    10261 cgccatccat ggcctgtctt ttcgcaatcg tataaatagt cgtcgtcaaa ttcacaatgc
    10321 cacctttcga tcccgcggta gcagatatta tcatcactat cttggcatat tgtcctgccg
    10381 tctcttttat agcactcata tccatcatcg ctcttatcta catacatacc tcttgctcct
    10441 gtcaaaatag tttcatcctg agtgctgctt ccttccacgg tttgataatc ctcgcctgcc
    10501 actatccccg taagcatgac tgtaacagca aataaaaagg tcatcttcat atttgatcac
    10561 cccttttttg caaatgattt ctggatattt atgcttattt ttgctggata gatgggtaaa
    10621 tagaaaaaaa agaaaataat ctagatatcg atcaatcctc ctatgattct gctgacccac
    10681 tcatcgtcgg acgaacttac ctcctcttct ttataatcat tgtcttccaa ctcttcatag
    10741 cagttgccat catcatcgca gaacatgtca gcgtcttcat attcgtcttc acagctgccg
    10801 tcctggcaat caaattcatc atcacagctg ccgtcctggc aatcctcttc gtcttcttcg
    10861 caagtaccat cctcactgca gtcaaactct tcatcctcat cacagctgcc atcgctgcag
    10921 tcaaactcat cctcatcaca gctgccatcg ctgcagtcaa actcttcctc atcctcatca
    10981 cagctaccgt cttcattgca gtcaaactct tcctcatcct cttcgcaact gccgtcttcg
    11041 cagtcagagg tctcttcatc tatacaaaac caatcgcagt cttctccatc acaccagtag
    11101 gagccatcat cgtcttctat gcaatcatca ccgtaacata atgaataatc gcagctgtca
    11161 tactcttcat cttctgcata agcgccttct tcttcatcat agccaggttc gtcctcatca
    11221 cagtttccat cttcatcaca aaacccttca taacttacat ccttgcatcc tcccatgttc
    11281 tcgcagtcat tttcctcata catgatccca agcgattcat catatgcatg aaggtcatcg
    11341 ctttcaaaat cgccatagtg gtccatctct gtgctgccgt ccttattatc tatgacgaca
    11401 aaagttatat agttgactcc tccattttct gcgctgatat gggagttagc gatcaccata
    11461 tcattagcat atcccataga tgccaaagca aacaaaaaca agaataagct tacaaataat
    11521 ctcttcataa taaccacccc ctctaggtgc tatgtttttg tcctatttaa ataaggagct
    11581 agatatgcta gcgatgctag agtcagagaa tatttttaat ctagaaaatc tttaataatg
    11641 aagtgatgta aatgcaaaca atgaaagtga catgcgagga tggctttgcc atacaaatta
    11701 aaaatgaaaa agaaataatc gagcacactc aaacccatat gaagaatttc cacaataaag
    11761 atatgagtgc agatgatata agggggatta tgaaagctgc ttaatggaaa tactgaaagt
    11821 aacctgtgag gatggctttg aagtgaggag cgtaaacgag aaagaggtaa ttgagattat
    11881 caagggccat atgaaaaagt ttcatggtgt agacctaacg gctgaagatg ttatcgagat
    11941 gatagaggtt gtccatcatc acagtgttga tcatcctgct ttctttttgt cagagtaaac
    12001 ttaaatttct tgttctttta gttatcaatc gccatttacc catgtcgttt gagcaaacga
    12061 tttttaatat cttttccgga tatggatgtc caaaacaaaa catttatata tcagtttata
    12121 caataatgtc tagatgcaaa aagcccttgg tgattatctg gatctgagtg cggtagttat
    12181 aagagcggac attttgcaat ttatcagaga atcaaagata gagttcaaat agagcatttg
    12241 ggggtaggtt aggtgcatca taatgactaa tgaagttggg ggaatactca ggcagcttgg
    12301 cataaagccg gcctatgtaa aacggttcaa aaaaggagct atcaacaaaa cctacgatat
    12361 ccaagctcaa cagaattatg tcctcaggat ctatcctcgc gacttctgga aagccaagaa
    12421 ggaagcttat ctctatcgcc tgcttgggaa aaagacagat gttcctgtcc caaatgtcat
    12481 aaaacagggc aggaattatc tattgatgca gaaggttgag ggtaaggctt tatctttatc
    12541 agatagaaag cttgtcaagg cttcaggaga gatgcttgca aagatacact ctgtcaagtt
    12601 cagctctttt ggatggataa taggccggca aatagagcca aagttcacta cctgggaaaa
    12661 atttgtcaag tatgacactg agaagaaact tggcaagata ccagggcatc tttttctaaa
    12721 aagggagata aaaaagataa tctttgacaa caatcatttg ttaaagatag ctgaaaagcc
    12781 ttgcttgctg cataaagact atcattcttc acatatcctt acccatgatg gaaggataaa
    12841 tggaataatc gacatggaat gggcgatgtc tggccataac gagctcgacc ttgccaaaac
    12901 ttttatctgg atgtttaatg gcaggccgag cctgcagaat atcttcatga aaggatacct
    12961 taagcatggc actgtttcaa agcaatttta tgagaggctg gcgctttatc gtatattgac
    13021 actactttct gctgtatctt tttcttatga atgcggcaat aaaaagtggt gcaaggttaa
    13081 tcttaaacaa ctaaaaggtg aacttaatga atatcacaaa actcactgag gaatatatca
    13141 acgagcatcc gagcataaag gattgtgtca ggaaagggct catgaactat tcttccttga
    13201 caagaaagat agtggaagat gagaaccttt cgttaaagga taactttgat gcaatactaa
    13261 tcgcatgcag aaggtattat gccaagataa agaaagaaga gatacttgag aacaagataa
    13321 tggacttgtt aaaaaaaagc aagcttgaag tcaagaataa gatagttgtc gtcgtcgttg
    13381 agaaggatgt ctatttcaac cacctcatag agctgcacaa ggatgtaaag aagaaagcag
    13441 gcgtctttca tataatagaa ggttcttcaa ccataacaat catcacgtca tctgagtttt
    13501 tggatgagat atctaaattg ttcaggaaca agataatcag agtgagtgaa aacttggctg
    13561 aagtaaactt aaaaagccct gaggagcttg agaagacgcc aggagttatc gcttatctat
    13621 ctactttact tgctgagaac gggataaata taatcgagac tatgtcgact tggactgata
    13681 ctttgtttgt gatatctgag gaggatatcg ctaaggttat gggcgtgctt aggttctaat
    13741 tatttattcc ttctataatg ttatgatatt atgagataaa ttgcttttca gcaggttttt
    13801 aatgtaacgc atagtttact acttttaaat gaaaatagcc ttggtagact taagtttgat
    13861 gtacagttgc tttgaagatg gatgtaaatg taatgccaat tcatcgctta ccctatcaac
    13921 aataaaccaa attatcgcta gatgtgacag ccatgaagtt tgttttgttc caacaaataa
    13981 tcacaatcct agttctctta ccgatcttat cgatacttgc tcaaagcaaa tggcagacct
    14041 aaaaccgcta gttattttca tatccgcttg ctctgcgtat gcaggtcttg cactaaatct
    14101 tagcaagaaa tggaagcaag aaattggttc aaaggttgtt ttaggtggaa ggcattttga
    14161 ggaaccaaga ctaagccatt attcacctca ggagttgacc ctagattctc aagttaggca
    14221 gccaattact acttcccttt tatggggtca gcaaaacaaa tgcctagatt atgtttattc
    14281 tggcagcggg ggaaatattc ctcatttatt gaaggctatt gaagacggct tgacagctga
    14341 tgcagagggc atatttactc tatcagggag atatcttcaa ggcaaaggta gatgcaggga
    14401 atttcctgca ggaattatcc ccatttcaaa agccaaattt gaggatgggg acgactatct
    14461 attaagagtt atagtatcag actactgccc agaaggttgt ggtttttgcg ttgggccttc
    14521 caggttagga gtttcgtttg atgaagccct gctttcacaa gccgcaacag caataatcgc
    14581 aaacacttgt gataaaacta caattataca gttttgtgat tctaatccta tcaggccaga
    14641 ttcaatagac aagtatgggc agataatcaa gcaagtaagg aaagcaaaac cctacaggca
    14701 gtatggtatg tttatgaata ccgcatacct tattgataga aggtttttcg attatacaat
    14761 gcagtttgtg cgcactatgg atataatgag cctttttgtt ggaagggact gtttaacagg
    14821 agaaattgct ggcgcaatag gcagaaacca taacaggctg tctcgcagcc aggaaatgct
    14881 tgacggggaa caaaatgtga tatgtaaaat actttcagct tccgaccaat atcaaaaatt
    14941 taatgatttc atacatactt gcacctcatc tgataacaag agaatatact caaaggatgg
    15001 ctgaagagat ggatatgttt gcaagaacag ctgtaaattc tggcgttaat ctaactattc
    15061 agctaggagt gcttcagcca tatcctggaa ctccattggc taactcacac atgagatatg
    15121 acgataagat tgagcatttc attgatggaa accataccca attctggcta agcaatgact
    15181 ctgcactggc taatatctta accactgtaa gaatcatggg ggagttaaaa agtcaatatg
    15241 aggggacagt aaaatttatt tttgatttgc actcaaaaaa tgagagagtt ctatatgaga
    15301 ctcctagcat gagagaggtc atgcaaaaat caagcggttt atatctatac agaaagtagc
    15361 atgatttatc ctactttcta acaaacccct tcagaatcca ataaatggcc taatttttcc
    15421 ttctttgtct tcaaatactt ttcagaatgc ttgcctgctt ttatcaccag cggaatcctt
    15481 tcaactatct ttaggttgta tctctcaagg ccagtaatct tctttgggtt gtttgtaagg
    15541 agctttatag tagtcaggcc aaggtcagat agtatctttg cgcctatagt ataatccctt
    15601 tggtcgggtt tgaagccaag ctgctcgttt gcttctactg tgtcaaagcc tttgtcttgg
    15661 agagcgtagg cttttatctt attcaaaagg ccgattcctc ttccttcctg cctgagatat
    15721 agcaggaccc ctccttccct tgcaatctgc ttcatagcct tatctagctg ttttccgcag
    15781 tcacatctta atgaatgcag ggtatctcct gtcaggcatt cggaatggac acggacaatc
    15841 atatcttttt ttacatcgcc cctgaccaga gcaacgtgat gatctccctt gtcatcctca
    15901 taaacatgca tgacaaaatc gccatattcc gtgggcaggg cagctgtagc tttcttgatt
    15961 atcatagaag gataggataa attctgcttt tataaaggtt taggaaatgt cttcatcaga
    16021 aggctcttca tcaacttcat catagccctg catgaatcct tcttcttcag gcgagacctc
    16081 atcatctgat cccaggtctt cttttccata ttcttcataa aggccgcttt cttcctcgtc
    16141 gtcttcgtaa attttttcct tttccatccc aatctggctc ttaggggata tttttatact
    16201 ttacgattat aatttatata aaatttaagg aagcttactt tggcaagatt gcatatgcct
    16261 gcacaaagcc gcttatcccc tttgcaatca tctcgactgc gacagcagtc agaaacaaac
    16321 caaagaccct tgataaaaca tctattatct ttagattgat cttatttatg aacattgcca
    16381 gggtgataaa tacaatgccc agcaaaagga atgccaaaat tagattgaat attatatcca
    16441 ggcggcctat ctcgtagacc ttgactgtga taaatgagat ggttcctggg cctgtcaaca
    16501 tgggagttgc gatgagtgca gccaaaccgt ctatgtctcc aacctcatgg cctccatttt
    16561 ttggccgtat catgttgata ccaagcaaga taaggactac accgcctgct atctttaagc
    16621 cattcaaggt tatgccaaag atgtcaaaga gcaagtgacc cgagatagct atgatgaaag
    16681 cgatgatgaa agcgccgatg actgacttga acgctataat cctgacgttg agcctgcgct
    16741 tgtaggctga gataaggaac gggactgatg caagagggtt aagaaggatg aatatctgca
    16801 tgatgagcat gaaatttatc atcgacataa ccctattttt ttattccgct gatatatata
    16861 gcttgcgaaa ggtgctatct ggggtagagg aatataatca gatagaaaat caacatcagc
    16921 acagccaaga ctactcctat ccaaagcaat cttctgtcag ttgcaaatcc aaaagggata
    16981 aatccgataa atcctccaac agctatcttt gactttccgg atgacaatat acccagaaga
    17041 ataagagcta gccctatgaa taatacgacg atgcccaccg tgatgagatg tttcatccca
    17101 gggtatctgg atgcaataga ttaatttcat tgtgcttctc taatatatat tttgcagcag
    17161 ctaaaaaaac caaaagcttt aaatatcaca aatatacaga tttgtatact agtttaacca
    17221 aatcatttca cagaaaatcc ctaaaaaaga ggcaagaatt ggctggtgag gcataaagag
    17281 ggaacgtacc tttatgcttc actgatcaca aaaaaaatgg atgccgagac caaaagtttc
    17341 atagagaatg tagacagctg gataaaacag atcaggtctg agtttaccca gataacggat
    17401 gtttccacca tagtcgaaga gaatgtcaac aatacgcagc ataactatga attgatatat
    17461 gagttaaaag aagagataga acaactaaaa tcagagatta agacccttaa gataatgcag
    17521 cttatggagc ttaaggagaa gcttgctagg cctgtgcaat aatcttcttt aatgcgctgt
    17581 ctgcagccat aaacacgttg caatcaactg attctgaaac caaaatgttt gcgccgagtt
    17641 taagttcaac ttttaccttg cagtcgttat cctgttcaag aagggtgatg gaaagcttct
    17701 catagtcctt tcttttatcc tgtatctgcc tgacataatt gccgataatc ttctttagca
    17761 ctatctgcct taagctttca acctttgaaa atcctactaa ttctatgttt ccaccaaggg
    17821 aaaccagatc ctcttgagcc ataaatcccc gagttaacct atagacgaga ctagtataaa
    17881 aaggttgctc ttttttatcg tcttttattc cgaggatagc tggaactata ctcaaagaca
    17941 aaatctattg agcaaactaa tgtcttttcg tattagcaga aagacctgtt tgattgctca
    18001 aaatattggg tcttttgcta tatcatccat taattttata aatgacttag ccttgcagaa
    18061 caggatgctt gttgaatctt tctcagggat aagggggata tatggcctgg accttacaag
    18121 tgatgttgca ggaagatatg cctttgcata ttataactat ctgagtcaaa agataggaag
    18181 agggccgata atagtagtcg gcagagacac aaggccctca ggcagcgctg tctataaggc
    18241 agtaatcgaa agcgtaacaa acgtactgga tgtcggaatt atacctatac ctgcattgag
    18301 ccttgcagtc agagagttta aggctgatgg agggataatg ataactgcat cccacaacga
    18361 accggagttc aacggcctta aattcttgtc atcggatgga gctgttcttg ttcctaacga
    18421 gatgcaaact gtgataaact catttagaaa agtcaaggat gttcctgcaa gaaagccttc
    18481 tattaaaatc aaagatatct catcggtcta ctccaaattt atcggcaacc ttacaggcac
    18541 tattgaaaat aattataaag ttatcgctga tgcaaacggc ggcacaggat atctgcttga
    18601 gaaggtggca aaagacctgg gtgttaaaaa tattagggtt ataaaccaag agcaagggat
    18661 ctttaagaga aaagtcgaac ctacaaaaga atcgcttgct tatcttaggg acatgataaa
    18721 agctgatgga gcggtgtttg cagcaggatt tgactgcgat gcagacaggg tcgagatcct
    18781 gcttgacgat ggaaccatcg tcgatgggaa tatgctgctt gccataatcg tggatcatat
    18841 ggttggaaat aagaaagggg ttgttgtcgt caatgatgcc acttcaaata ttatcaggga
    18901 tatatgtgca aaacatgggt gcctgcttaa gga
//
LOCUS       DASRUK010000045         2104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1716333, whole genome shotgun sequence.
ACCESSION   DASRUK010000045 DASRUK010000000
VERSION     DASRUK010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1716333"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VFF28_02060"
     CDS             <1..437
                     /locus_tag="VFF28_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44446.1"
                     /translation="KYRQIQCMYADCLQTGVGKQGLPVFACEDQKEYATCKYVVGEVF
                     QVIPFTALWTYYLNLIRATLSDPFKIVGAGIAATCRFLITPESPYGYHLCAGTKVLSH
                     LGITIQEITSIFDSDTWKIHDDFCSKLQQSEEPESESGGLFG"
     gene            438..>2104
                     /locus_tag="VFF28_02065"
     CDS             438..>2104
                     /locus_tag="VFF28_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44447.1"
                     /translation="MKRKKMSRKGKQFGTILIALAFIISLFSIAFYTENGNFIDYGNQ
                     NSGITGAVVGMESGTGADSAIEQIDKKVQDKISKSASGATTIQKSKFASSKELSDLET
                     LGLKFASCKTDDKCSISTLDQLDKQYEIRRQEILLREETAENLFEKSQDNTDKDQQLA
                     KKALNWQLKDTKETDIRDGAIAHLIKGGGTAGRQLLASTAAGSNQLQKYLTENYKEDY
                     TKLKVDLEVARAAQSKGYAYDQNSGNYYDPKTGDVYRPIETTNGVQQFIKTTNKVDIA
                     TVSGANTDQKIKDLEASLGMNKYDYNGPIKSLEVVGTTIMAKDINGFLYPIDATKKEV
                     IYSAKESPTSIKIGKSSLKRVDNYMQVDGSDELAVYKAADGTFYNQRNEKVTSVIKTE
                     KHGNIRILEEIQVTNGKLEKKILSTKIQSGTSEPIAIDPIVKAQVVSAIKSAGKEAVI
                     EGSLDEGSELKISNKDEKLISQITKTQSGDDEIDGFKIETLFTDDGQALNVFRTSPEG
                     KVTVKETKVVKEIDTDNKDADGKPIKEKTIVTKEWVKLDEANKEKTVR"
BASE COUNT          758 a          375 c          511 g          460 t
ORIGIN      
        1 acaagtacag gcagatacag tgcatgtatg ccgactgttt gcagaccgga gttggaaagc
       61 aaggattgcc tgtctttgca tgcgaggacc aaaaagaata tgccacgtgc aagtacgttg
      121 taggggaagt tttccaggtg attcctttta cagcattatg gacttattat cttaatctga
      181 ttagagccac attatctgat ccatttaaga tagtaggggc gggaattgcg gcaacatgca
      241 gattcttgat aactcctgaa agcccatatg gttatcattt atgtgcaggc accaaggtgc
      301 tttctcattt aggcataaca atccaggaaa taacctcaat tttcgactcg gacacatgga
      361 agatacatga tgacttttgc agcaagcttc agcagagcga ggagcctgaa agcgagagcg
      421 gagggctctt tggctagatg aagcgcaaaa agatgtcaag gaaagggaag cagttcggca
      481 cgatcctgat agctcttgcc tttattatat ccctattcag cattgcgttc tatactgaaa
      541 atggcaattt tattgattat ggtaaccaga acagtggaat cactggtgct gtggttggga
      601 tggaaagcgg gacaggcgca gattctgcca tagaacaaat cgacaaaaag gtacaagaca
      661 aaataagcaa aagtgcatca ggagcaacta caatacagaa atccaagttt gcatcttcta
      721 aagaattgag cgatcttgaa actttaggcc ttaaatttgc atcctgtaag acagatgata
      781 agtgctcgat ttcaacgctc gatcaattgg ataaacaata tgagatcagg agacaggaga
      841 ttctacttag agaagagaca gcagaaaatc tttttgaaaa gtcccaagac aatacagaca
      901 aagaccagca gctcgcaaaa aaggccctga attggcagct aaaagatact aaggaaacgg
      961 atataaggga tggtgctata gcacatctca ttaagggggg aggaaccgca ggaaggcagc
     1021 ttttggctag cacagcagca ggcagcaacc aactgcaaaa ataccttact gagaattata
     1081 aagaagatta taccaagctt aaagttgatc ttgaagtggc aagggctgca cagtcgaaag
     1141 gctatgcata cgatcaaaat agcggaaatt actatgatcc aaaaacagga gatgtgtaca
     1201 ggccgattga aacaactaac ggtgtgcagc aattcataaa aaccaccaat aaagtcgata
     1261 tagctacggt ttctggtgct aacaccgatc aaaaaataaa ggatctagaa gctagtctcg
     1321 ggatgaacaa gtatgattat aatggaccta taaagtcttt agaggttgtc gggacaacga
     1381 ttatggccaa agacataaat ggtttcctct acccaattga tgcaaccaag aaagaggtta
     1441 tatattcagc aaaagaatcc cccacatcga taaagatagg gaagagtagc ttaaagcgag
     1501 tggataatta catgcaggtg gacgggagtg atgaattggc ggtgtataaa gcagcagatg
     1561 ggacatttta caatcaaaga aacgaaaaag ttacaagtgt catcaaaaca gagaaacatg
     1621 ggaatatccg gatattggaa gagattcaag taactaacgg caagcttgaa aaaaagatat
     1681 tgagcacaaa gattcagagc gggacctctg agccaatagc tatagacccc attgtaaagg
     1741 ctcaggtagt cagtgctatc aaaagcgcag gcaaagaggc agtgattgag ggcagccttg
     1801 atgaaggaag tgaattgaag atatcaaata aggatgaaaa gcttatctca cagataacca
     1861 aaacccagag cggcgatgac gagatagacg gattcaagat agaaacgctg tttaccgatg
     1921 atgggcaagc tctgaatgtg ttcaggacaa gccctgaagg aaaagtgact gtgaaggaaa
     1981 caaaagtggt caaggagata gacaccgaca ataaagacgc agatggcaag cccataaaag
     2041 aaaaaaccat agtgacaaaa gaatgggtta agttggatga agctaacaaa gaaaagaccg
     2101 tgcg
//
LOCUS       DASRUK010000046         7714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1385492, whole genome shotgun sequence.
ACCESSION   DASRUK010000046 DASRUK010000000
VERSION     DASRUK010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 7714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7714
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385492"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VFF28_02070"
     CDS             complement(<1..791)
                     /locus_tag="VFF28_02070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZX44448.1"
                     /translation="MFRRSQLEGVFLLIFITTFLLLGLSNKWDAKLAHPMPYGYGASD
                     AFFHASVAEYMVEQGRVKYTPPYEVGGYEKVLDAHPPMLFQLTGIFSTFTGSAAYDSI
                     YFITVIAYLLAILVLYLIIRQYSKNIAIISLPFGLLILSNKFSISLHWGYWLLIMGIL
                     FMIFSLWALTRIRLKHSYIILALALGSLLIAHQPEGVFIIGFIMLYLGADALFNRRID
                     KTSLKSVVFGLLIALVLAAYSLNIFYNTFMQTEGYRGKFDTEFGA"
     gene            864..1814
                     /locus_tag="VFF28_02075"
     CDS             864..1814
                     /locus_tag="VFF28_02075"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HZX44449.1"
                     /translation="MASILVTGGAGFIGSHVCEALLNRGDSVICIDNLDPYYSPSIKE
                     KNLEVCKKSSNFSFVKLDLLDKESLDEFFDKNPVDKVIHLAAKAGVRPSIKDPAGFAK
                     VNILGTINLLEAVKERKLANFLFASSSSVYGDSSLPFSEDSKVDSPISPYAATKKAGE
                     LLCASYHNLYHIPITCLRFFTVYGPRGRPDMAPFKFTKAILEGRQIELYGDGSSRRDY
                     TYITDIVSGLLSALDKNLDFEIINLGESHTVELKEFIKVIEKALGKKAKVNHVQSQPG
                     DVKATYADISKAKALLGYDPKIRIDEGMRLFAEWLKVARC"
     gene            1808..2656
                     /locus_tag="VFF28_02080"
     CDS             1808..2656
                     /locus_tag="VFF28_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZX44450.1"
                     /translation="MLSIIMPVFNEEGSVENTIDELKKEMERLKVAYELIVVDDGSTD
                     KSSQILKNIRKIKLMTHPYNLGYGASLKTGLKVAKYSYILIIDADGTYPIMDISKLLR
                     HSKSYDMVVGARTGRKVSDPILRKPAKLFLRTLARLLTGRNIPDINSGFRIFRKDVAM
                     EFFHLFPSGFSFTTTITIACLTNDYTVKYVPINYFKRKGTSTIHPIKDFVGFTTLMFR
                     LIMYFNPLRIFLFPSLFLFVIGLIILLYDALIRVDITDLPIIVLLAAFQLGFLGLLAD
                     LIVKRK"
     gene            complement(2636..3553)
                     /locus_tag="VFF28_02085"
     CDS             complement(2636..3553)
                     /locus_tag="VFF28_02085"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HZX44451.1"
                     /translation="MKKKYKVIAELLLIILIFSLFYKFMNLSYSFTYLANANFGYFII
                     AIVIAFGTRFVNASRFYLLAGSIGVHLGYKKNLMVHFLTGFLGRFTPARVGEGSKVML
                     LEGDKQKIGFLFILEKIVDLITLVIISVIAIFTFSMYGSIGIKLMIFLGFFTIALVLL
                     FHIEKIINLVMKRAVLEKQWFYKNLKSIPKKAFVIFLFNSLAIRFLVLSIPFLVAIAL
                     DIRISYWLIFQMYAVSIIAGWLSGLPGGMGTREVAYSFLLMAYSITKELAGIAAILVI
                     LVDLLVESVLAIAGYLALKVGRKSLPLNN"
     gene            complement(3525..5129)
                     /locus_tag="VFF28_02090"
     CDS             complement(3525..5129)
                     /locus_tag="VFF28_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44452.1"
                     /translation="MKIALIAGIAITVAFCLSAFKLFPVQEYMKVSSRNLSYQESSGR
                     AETLAHLVPSIISIVGIVPLGLILFGVYKGWKKKTIIFLVAVCLFSFLVSMRGPILYL
                     VWKYYPSWQGMRYASRGMVLFAFAGSILAGAGLKEINEYVKNKSKKIALIHIVIILLT
                     IISLLVLGKGPIRIGSDGKQYEYGNIDEMIKQNEVMQAISKMPGIFRMNVFETRGIDW
                     GTEFYTVPLKLEPIFGYETAWLTEYMNEYLSLAYNDLPKFWGILNVKYITSKERINLT
                     GFEMIGEYKPCSICFPSIPELAKAYGPYLFENKKFLPRAYIVNNGILVVGEKASAKDA
                     MYSLMLHPAFDPSRSVIVLGKEKINQYDIGSLQRFSHVILTKGSVDGEDRILAEYKRK
                     GGIILPDVLVGKNSISEEDVGRMFSTNSSFVPVADKNILTVNFDNKKIALEKEQSGFL
                     VVSEQYSIYPGWGATVDGKPAEILRADGVISAVVLDNASKLTFEYKPKSLQLGVIISI
                     IAAIAIAIYMIYFIYRNYEKKVQSNS"
     gene            complement(5205..5786)
                     /locus_tag="VFF28_02095"
     CDS             complement(5205..5786)
                     /locus_tag="VFF28_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44453.1"
                     /translation="MDERIKHLINIFLLAVITLTFLYPWLNTAEHHSGDIVLYYLPRV
                     EFLKQSIVEYSQFAPLWTPYYMGGASYAGKPISPTFNYILGSLLLVMPTALGAIKYSI
                     ILGIILAGIFMYILTFHLLQDKKAAFLSAIVYMFNGYVIENIIHSGWMVFAYTYPLIP
                     LLVYFSFKAISKKYVFYSVLAGIVLALMIHAGA"
     gene            complement(6077..6994)
                     /locus_tag="VFF28_02100"
     CDS             complement(6077..6994)
                     /locus_tag="VFF28_02100"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZX44454.1"
                     /translation="MFLLVYGQYNQHLSFKDIEVNMILVTGATGFIGKRLVRRMDAKK
                     AVLMVRNRIESRFRQRIASLDDENSLYEATKGIEVVIHLAAAVGIVDPAANYRINFEG
                     TRKLVGACKKNKVKKIIFLSSVSAVQKKREAYGQSKLQAEKIIIDSGIDYVILRPTLV
                     FGESRQFKVIKDIIAKYPVIPIIGSGKSIMQPISVDDLVEMIIMSLGMKNEIINLAGP
                     SRITFNQMISKIASSMGIKKPMLHLPKSLCLIAAFFLEKALRSPPITRNQVYALTQDS
                     TTDISDMLAKFKLKPKTFDDMVKNFTKCC"
     gene            complement(6934..>7714)
                     /locus_tag="VFF28_02105"
     CDS             complement(6934..>7714)
                     /locus_tag="VFF28_02105"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZX44455.1"
                     /translation="PLLRPDICILINEAKKQGINVNINTNGSLLKNRAKELVASGVDT
                     ITVSLDSHDPDVHNSVRGFKGLFEKAEQGISEIRKLRKDRRPHLMARFTVNKRNYKGI
                     QPYIEYWKDKVDKVTLQPVYEGESTSFFHIKDKGIMFEEKDRKEFTDYFTRLLKRYKW
                     FNKIYYKEFPTFFFQKKELQKRYRCFSPYFYLVIDAYGNAFPCAMLIRNFGSLREKSL
                     KQVWNSREVNSFRRMIKQRKNRCFCWSTDSIISTYLSRILR"
BASE COUNT         2279 a         1585 c         1551 g         2299 t
ORIGIN      
        1 ttagctccga attctgtatc gaatttgccg cgatatcctt ctgtctgcat gaatgtgtta
       61 taaaaaatat tgagggaata tgctgccagg acaagtgcga tcaatagccc aaagacaact
      121 gactttaaac tggtcttgtc tattcttcta ttaaataatg catcagcgcc cagatagagc
      181 atgatgaatc cgatgataaa aaccccttct ggctggtggg cgatcagaag agaaccaagg
      241 gctagcgcaa ggatgatgta ggaatgcttg agtctaattc ttgttaatgc ccaaagagag
      301 aaaatcatga ataggatgcc catgataagc agccaatatc cccagtgaag gctgatcgaa
      361 aacttattgc ttagtataag aaggccgaat ggcagcgaga taattgcaat atttttgcta
      421 tactgcctga ttatcaggta tagcactaag atagcaagaa gatatgcgat tacggttatg
      481 aagtagatac tgtcatatgc tgcagagccc gtgaatgttg aaaagatacc agtgagttgg
      541 aacagcatcg gtgggtgagc atcgagcact ttttcatacc cccctacttc atagggagga
      601 gtgtacttta cccttccttg ctcgaccata tattctgcga ctgacgcatg gaaaaaagca
      661 tcagaagcac catagccgta tggcatagga tgagcaagct ttgcatccca tttatttgaa
      721 aggccaagca ggaggaatgt ggttataaat attagcaaaa aaacaccctc aagttgtgat
      781 cttctgaaca taggctgagt gtatgtcaag actttttata tatttcgtta agtttataaa
      841 ttattttaaa agcctatgat attatggcat caatccttgt tactggtgga gctggattta
      901 tcggatcgca tgtttgtgaa gcgcttttga atagaggaga tagcgtaatc tgcatcgata
      961 atcttgatcc ttattactct ccttcaatca aagaaaaaaa tcttgaggtg tgcaaaaaaa
     1021 gcagtaattt ttcttttgtc aagcttgacc tgttggataa ggaaagcctt gatgaatttt
     1081 ttgataagaa tccagtcgat aaggtcatcc accttgccgc caaagcagga gtaaggcctt
     1141 ctattaaaga tccggcaggc tttgccaagg taaacatcct ggggactata aacctgcttg
     1201 aggcagtcaa ggaaagaaag ctcgcaaatt ttctctttgc gagctcatca tctgtttacg
     1261 gtgatagcag cctgcccttt tcagaggaca gcaaggtgga cagcccgata agcccctatg
     1321 ctgcgacaaa gaaagcaggc gagcttttat gtgcatctta ccataacctt taccatatcc
     1381 cgattacctg cctaaggttt tttactgttt atgggccgag aggaaggcca gacatggcac
     1441 ccttcaaatt cacaaaggct atccttgaag ggaggcagat tgagctttat ggtgatggca
     1501 gctccaggag agattacacc tatatcactg atattgtctc aggcttgttg tcagcgctcg
     1561 ataagaacct tgattttgag attataaatt tgggagaatc acacactgtt gagttgaagg
     1621 aattcataaa ggtgatcgag aaagctcttg gaaaaaaagc aaaggttaat catgttcaaa
     1681 gccagcctgg agacgttaag gccacttatg cagacatatc taaggcaaaa gctttgcttg
     1741 gctatgatcc aaagataagg attgacgaag ggatgaggct gtttgcagag tggctgaagg
     1801 tggctcgatg ttaagcataa taatgcctgt atttaacgag gaaggcagtg ttgagaatac
     1861 gatagatgag ttaaaaaaag aaatggaaag gctcaaggtt gcttatgagc ttatagtggt
     1921 agatgacgga agcactgaca aaagcagcca gatccttaaa aatatcagga agattaagtt
     1981 gatgactcat ccttataacc tgggatacgg cgcaagcctc aaaacaggat taaaggttgc
     2041 aaagtatagc tatatcctca tcatcgatgc agatggtact tatccgatca tggacatctc
     2101 aaaacttttg agacattcca aaagctatga catggttgtt ggagcaagga caggaaggaa
     2161 ggttagtgac ccgatcttaa gaaaacctgc aaaacttttc cttaggacac tggcacgctt
     2221 gcttactgga agaaacattc ctgacatcaa cagcggattt aggattttcc gtaaagatgt
     2281 tgcaatggag ttcttccacc ttttcccgag cggattctcc tttaccacga cgataactat
     2341 cgcctgtctt acaaacgatt atactgtcaa gtatgtgccg ataaactatt ttaagagaaa
     2401 gggaacatcg accatacatc ctataaagga ttttgtcggc tttacaacct tgatgtttag
     2461 gcttatcatg tactttaatc cattgcgcat ctttttattt ccaagccttt tcttgtttgt
     2521 gataggactc attatactac tttatgatgc tttaatcagg gtggacatca ctgacttgcc
     2581 gataatcgtt cttcttgcag cctttcagct cgggtttcta gggcttcttg cagatttaat
     2641 tgttaagagg aagtgacttc ctgccaacct tcaatgccag ataacctgct attgcaagga
     2701 ccgattctac tagtagatca acaaggatga ctaatatcgc agctattcct gcaagttctt
     2761 ttgtgattga ataagccatg agtaaaaaag aatatgcgac ttcccttgtt cccatgcctc
     2821 ctgggaggcc agaaagccat ccagcgatta tcgaaacagc atacatctgg aaaataagcc
     2881 agtaggagat tctgatatcc agtgctatcg cgaccaaaaa gggtatgctt aagaccaaaa
     2941 atcttatggc cagcgaatta aacaggaata tgacaaacgc ttttttcggg atagacttaa
     3001 gattcttata gaaccattgt ttttcaagga ctgctctttt catcaccaga tttattatct
     3061 tttcaatgtg aaacaggaga acaagcgcta tcgtgaaaaa acctaggaag atcataagct
     3121 ttattccaat tgatccatac atcgaaaatg tgaagattgc gatgactgaa ataatcacca
     3181 atgtaatcaa gtcgacgatc ttttccagga taaacaggaa gccgatcttt tgtttatctc
     3241 cttctaatag catgactttt gagccctcgc ctacgcgtgc aggagtaaat cttcctagaa
     3301 atcctgtaag aaaatggacc attaggtttt tcttgtatcc cagatggacc cctatgcttc
     3361 ctgccaggag gtaaaatctc gaggcgttga caaagcgagt gccaaaagcg atgactatag
     3421 caattataaa gtatccaaaa tttgcatttg caaggtaggt gaatgaataa gagagattca
     3481 taaatttata gaaaagtgaa aaaatcagga ttataagtag cagctcagct attactttgt
     3541 actttttttt catagttacg gtaaataaag tatatcatat aaatagctat tgcaatagct
     3601 gcaattattg atattataac gcctagttgg aggctttttg gtttatattc aaaggtcagt
     3661 tttgacgcgt tgtctaacac gaccgcgctt atcacaccat cagctctcaa tatctctgca
     3721 ggttttccat caacagttgc tccccatccg gggtagatag aatattgctc gctgactaca
     3781 agaaatcctg actgttcctt ttcaagggct attttcttgt tgtcaaagtt gacagtgagg
     3841 atattcttgt ccgccacagg cacaaaggat gagtttgtgc taaacattcg gcctacatct
     3901 tcctcgctta ttgaattttt gcctacaagc acatcgggaa ggatgattcc tcctttgcgt
     3961 ttatattcag caaggattct atcttcccca tcaaccgaac ccttggtcag tattacatgg
     4021 ctgaaccttt gtagggatcc aatatcatat tgattgattt tttctttgcc aagcacaatc
     4081 actgatcttg aaggatcaaa ggcagggtga agcatcagcg aatacattgc atcttttgct
     4141 gaagcttttt cacctaccac taatatcccg ttatttacaa tgtatgctct aggcaggaat
     4201 tttttgttct caaacagata tgggccatag gctttggcta gttcaggaat agacggaaag
     4261 catatgctac acggcttgta ttcacctatc atctcaaatc cagtcagatt tatcctttcc
     4321 ttcgatgtga tatacttgac gtttaatatc ccccaaaatt tgggaagatc attatatgca
     4381 agggacaggt attcattcat gtattcagta agccatgcag tctcatagcc gaaaattggt
     4441 tcaagtttaa gcggcacagt ataaaactca gtaccccagt cgatgcctct tgtctcaaac
     4501 acattcatcc tgaaaatccc tggcatcttg ctaattgctt gcattacctc attttgcttg
     4561 atcatctcgt caatattgcc atactcatac tgtttaccat cactgccgat cctaatcgga
     4621 cctttgccta ggacaagcag gctaattatc gtaagtaata tgattactat atggatgagt
     4681 gctatttttt tcgatttgtt cttcacatat tcatttatct ccttaaggcc agctccagca
     4741 aggatggagc ctgcaaaggc aaaaagaacc atgcccctgc ttgcatatct catcccttgc
     4801 cagctaggat aatatttcca gactaaatag agaattggtc cgcgcataga aacaagaaat
     4861 gagaataggc aaaccgctac aagaaatatg atcgtcttct ttttccagcc tttgtatact
     4921 ccaaatagga taagtccaag tggaacaatg cccacgatac tgattatgct gggaacaaga
     4981 tgggcaagtg tctctgccct tccgctgctc tcctggtagc ttaggttcct gctgctgact
     5041 ttcatatact cctgtacagg aaaaagcttg aatgctgaaa ggcaaaaagc gactgttata
     5101 gcgatccctg cgataagggc gatcttcaat aacttttgtt tgcttaatga gacctttgat
     5161 aataggaaaa tcgcaaagat ctgcgtgctg taaagaaaga ttattcaggc accagcatgt
     5221 atcataaggg caaggacaat gcccgcaagg acagaataaa agacatattt tttcgaaata
     5281 gccttgaagc tgaaatagac cagtaaggga attaacggat aagtataggc aaacaccatc
     5341 catccggaat gtataatatt ttcaatcaca tatccattga acatgtaaac aatagccgat
     5401 aggaatgcag ctttcttgtc ctgcagcagg tgaaaggtta gtatatacat aaaaatccct
     5461 gcaaggatta ttcccaggat aatgctgtat ttgattgcgc caagcgcagt aggcattaca
     5521 agcaataatg accctaaaat atagttgaac gttggagaaa ttggctttcc agcatagctt
     5581 gcgccaccca tgtaatacgg ggtccataga ggagcaaact ggctgtattc aactatcgac
     5641 tgctttagaa attcaactct tggaagataa tataggacaa tatctcctga atggtgctct
     5701 gctgtgttga gccaggggta aagaaaagtg agagtgataa cggcaagcaa aaagatattg
     5761 attaagtgct ttattctttc atccatggaa aaatagcaaa ggcgcttgat ttataaactt
     5821 ttttaagcta attaagctaa acatgaacct ctttatcttc ggtgggcgtg caatgagcaa
     5881 atggcttttg caataaactt aacaggcaac caattagctg gagtatttga tagaatagcg
     5941 gtaaaagata ataaacgata gctcttcaac tatgtttcat caggagaaac acagataagc
     6001 aatcttttta acatccgctg atggagtatt tactgcaatc ccaaaacaaa tggggcagta
     6061 atcgagcatg gaataatcaa cagcacttgg tgaagttttt gaccatatca tcaaatgtct
     6121 ttggctttag cttgaacttg gccaacatat cagaaatatc agttgtcgaa tcctgggtaa
     6181 gggcatagac ttggtttctt gtaatgggtg gactccttag tgctttttca aggaaaaatg
     6241 cagcaatcag gcaaaggctt tttggcagat gcagcatagg cttttttata cccatacttg
     6301 atgcaatctt ggatatcatc tggttgaacg ttatccgcga cgggcctgcc aggtttatga
     6361 tctcattttt catgcctaaa ctcattataa tcatctccac caggtcatcg acacttatcg
     6421 gctgcattat cgacttgcct gagccaatta tcggtataac cggatacttt gcgatgatat
     6481 cttttatcac tttgaactgc ctgctttcgc caaagacaag agttggccta aggatgacat
     6541 agtctatgcc agaatctatt attatctttt ctgcctgaag ctttgactgg ccataagctt
     6601 ctcttttttt ttggactgcg ctgaccgagc taagaaatat tattttcttt accttatttt
     6661 tcttgcatgc gccgacaagt tttcttgtac cttcaaaatt tatcctgtaa ttggcagcag
     6721 gatcaacaat acctacagct gccgcaagat ggatgacaac ttctatgcct tttgtcgctt
     6781 catatagcga attttcatca tctaggctgg caatcctctg cctgaagcgg ctttcaatcc
     6841 tattcctgac catcaggaca gctttctttg catccatcct tctaacaaga cgctttccaa
     6901 taaaacctgt cgctccagtt accagtatca tatttacctc aatatccttg aaagataggt
     6961 gctgattata ctgtccgtag accagcaaaa acatcggttc ttcctttgct ttatcatcct
     7021 gcgaaaactg tttacttcac ggctgttcca aacctgcttt aggctttttt cccttaggct
     7081 accaaaattt ctgatcagca ttgcgcaggg gaaggcattg ccatatgcat ctatcacgag
     7141 gtaaaagtaa ggcgaaaagc acctgtacct tttctgcagc tctttttttt ggaaaaaaaa
     7201 ggtaggaaac tctttgtaat atatcttgtt gaaccattta taccttttca gaagccttgt
     7261 gaaataatct gtaaattcct tcctgtcctt ttcttcaaac ataatccctt tatcttttat
     7321 atggaaaaag ctggtagatt caccctcata tactggctga agcgtcacct tgtcgacctt
     7381 gtccttccaa tactcaatgt agggttgtat ccccttgtaa tttcttttgt tcaccgtaaa
     7441 gcgcgccatt agatgtggcc ttctgtcctt tcttagcttt cttatctctg agataccctg
     7501 ctctgccttt tcaaataagc ctttgaatcc tctcactgag ttatgcacat cgggatcatg
     7561 actatccaaa cttacagtta tagtatccac gcctgatgcc acaagctctt ttgccctgtt
     7621 cttcaacaga gagccgttgg tattaatgtt gacatttatg ccttgcttct ttgcttcatt
     7681 tatcaaaata catatatctg gccttagcaa cggc
//
LOCUS       DASRUK010000047        19680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1218154, whole genome shotgun sequence.
ACCESSION   DASRUK010000047 DASRUK010000000
VERSION     DASRUK010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 19680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19680
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218154"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VFF28_02110"
     CDS             complement(<1..701)
                     /locus_tag="VFF28_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44456.1"
                     /translation="MPSESITCRHCESRDILKKGYRKNKMGQVQKYCCMGCGRYFTGK
                     KMLNKSYPAKIILSAISLYNKGMTLEQASLEINKRFHVKTYPKLISTWLKEFSKICTF
                     RRIRGKVREGRKSGFSSPVLEKLFDHKQPYLYKCHKMKIGMFLTEYYLGLKEYLLNVQ
                     SLCPHEMFLEDNARGSRIKVNDSKARVVKKKNYACELSGLALKMAKTNRERHGIIQDF
                     MLANDTSTIAVEVPV"
     gene            complement(691..2055)
                     /locus_tag="VFF28_02115"
     CDS             complement(691..2055)
                     /locus_tag="VFF28_02115"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HZX44457.1"
                     /translation="MDESEFNLILEEGEGQFIEFKESFDPKNLSKEICAFANSEGGQI
                     FIGINDRMEVKGITISNRLKSEIQDIARNCDPAASIWIEEYRGILIIHVLEGKNKPYQ
                     CSSGFYLRTGANSQKLTRDEIIEFSVGEGKIKFDEQINPEFRFPEDFDARKLDAYLSL
                     SRLTKNTPPEDILVSLKAAKPVKNKIHMTNAGVLFFAKSPEIFFINSKVVCVNYQTNE
                     KVNILDKKIFDNGIMDNIKEAINYVKKHINVRFEIKTALRNEILQFPEEAFREAIVNA
                     IMHRDYFDRSGDVLIEVFRNKIAISNPGGLVKWLKPEDFGRYSRARNQIIAELLSKTD
                     YVEKVGTGINRIRDSMKRAGLPEPIFDFNTSFAVTLLDGNTYGGVNGGVNGGVNGGVN
                     GGVNGGVNLLEFVGKNPGKRTSQMTKEMGMAKRTLERQLRKLKDDGKIEFRGSPRKGG
                     YYAK"
     gene            complement(2048..4249)
                     /locus_tag="VFF28_02120"
     CDS             complement(2048..4249)
                     /locus_tag="VFF28_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44458.1"
                     /translation="MVETMTAALYRGKGIFALVLVLFALGLFIADKYTGLAVYTSDIS
                     VTKEINFSAISSAEYPLFIDGILKGLSIDGQIAGKARVYLDAGNKSYLVYDNSEEGLL
                     SGYATIPLNGGGSKPLDGIFRFDVSSNRSGEKVCVRWSVNSYEMCNGDFDCCALLGLS
                     SSGEWNDSLYLTYERYGSSYENEVRAVMVFANYSLDPSNAYSDVSYSDEMLAYAVFEP
                     FVSFTSECLETCLLPSLNYSEYVLRVEIENGSLDIDSIHYVLEEEIPGHMPVLVKPFE
                     NITIIKGENVLLNLSAYFSDEDNDITLFYSNAVENVSISVYGDVAVVLPANFTGVRYV
                     YFSASDGYYTTTSNIFEIKVIDRPVAAAFANVTISEVNPEIRINEPVRWVKRVDTSSY
                     VSNLSLNISSDALNVSVYTEDERLSDEKVTVVVDGVEKEASTFIAEKKIGQIEKMTTK
                     LIAKKEEIIQERPTAAGEIASINKELSELSSEKNKLTGHAVVSSAEGLFTRFFEWLLN
                     IDITGYAVKENEDKDKKDKDDKDKPKDIEVIIEEPVSNVTVEYYTEAPISTEIVLSAT
                     VKQIVVSSDVHYENITAFTNVTPETISIRLYRLVNEIREEAYFDRYDNNNNSLTDYIE
                     WVVPSLSNQTYELVIEITKAEHLDENRAFVDDVYDYVKVQDDNWTLIPSGDYLRVTFE
                     ENLTAERDITIYARDSRIVNDSIMINGTLVPLDAYQKKMRLDEIRRMLANG"
     gene            complement(4234..4446)
                     /locus_tag="VFF28_02125"
     CDS             complement(4234..4446)
                     /locus_tag="VFF28_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44459.1"
                     /translation="MRRELDGTSKKILDIITNAGEPLETREIELLLKGISRVKALYRL
                     NNLRGEGLIKGKAVGSGKGTWIWWKP"
     gene            4576..5823
                     /gene="cca"
                     /locus_tag="VFF28_02130"
     CDS             4576..5823
                     /gene="cca"
                     /locus_tag="VFF28_02130"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR03671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HZX44460.1"
                     /translation="MKQILAEILKDIKPSSKEEKAVKDKADAVIKTINSGLRDAKAIL
                     GGSGIKGTWLRQANDIDIFVKYNYQKYKEKSGQLSSLLESHLKKKGKKIDKLHGSRDY
                     FQIKEDGYTIEVVPILDIKDAKAAKNITDISPLHAIWVNKKGKNLKDDIRLLKQFCKA
                     SKIYGAESYIQGFSGYICEILIIYYKGFENLIKAAAKWKDNITIDVENHWKKKNILLE
                     LNRSKISSPLIIIDPVQKERNAAAAVSKENVGIFKRRAKEFLKKPSTDFFKIQPLDKG
                     SLKKRAKDNILIMLEAVPKKGKVDVVGCKIVKAFEHIKAEFAKNDFKVLSQGWEWNKT
                     AFIYFIIKKQQLSDYTEREGPPLSLKFHADKFKKAHKDTFIKNNRLYAKMQRQFKKPE
                     DLIESLKGSQYIKEQVQELRWKG"
     gene            5811..6404
                     /locus_tag="VFF28_02135"
     CDS             5811..6404
                     /locus_tag="VFF28_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44461.1"
                     /translation="MERIRLEIKRQPIHMMLGFILIALIKYDLLSPIPFAIIILTAIG
                     SSLYLKKKKPKRIYNLLRYVERPDALENFPGKGLITYLIGALIAIVLFEKSTAMAAII
                     ILALGDSVSRLVGPFGRIKHPFNDARFLEGVIAGFIAGALGAMIFVSPSQAITASFVA
                     MIIEGIDLKLFGIKVDDNIIIPSLAGAAVWLMGIIAL"
     gene            6730..7326
                     /locus_tag="VFF28_02140"
     CDS             6730..7326
                     /locus_tag="VFF28_02140"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZX44462.1"
                     /translation="MKLLAFVDLHGNQKALKDIQKKSRHADMIVCAGDISLFENSLDS
                     LLLQLELLKKPVIIIPGNHEDEHDLRLASSVFGNIHDINNRSYMIGDYLFLGYGGGGF
                     CRVDKKFDKIAKKFTLIIKKNKGKKIILVTHAPPYRTNLDNIEGDSCGNKAIRNFIDK
                     VKPDLVISGHLHENAGKGDKIKNTRLINPGPFGKIIVI"
     gene            complement(7319..8227)
                     /gene="twy1"
                     /locus_tag="VFF28_02145"
     CDS             complement(7319..8227)
                     /gene="twy1"
                     /locus_tag="VFF28_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-demethylwyosine synthase TYW1"
                     /protein_id="HZX44463.1"
                     /translation="MIVESKKENLYQQGYRIVGKHSAVKPCLWCKKSLLDSGHCYKEE
                     FYDTLSHRCVQMTPALGFCGHRCVWCWRDIEFTLPKWKGKEDDPSEIVDECIAANAKY
                     LQGFGGNDKTNRKKFGELSNPQQFAISLSGEPTMYPKLPELILELRKRKINQFLVTNG
                     TNPSMIKKLIAAQPTQIYITLPAPDEETYLKVCKPLIKNGWAKILKSLSLLSKFKRSV
                     VRLTLVKGVNMKCPEQYGALVKKYRPKYIEVKAYMHVGYSQKRLKIENMPLHSEIAEF
                     AAQIALASGYKIKDEKKDSRVVLLSR"
     gene            8280..8777
                     /locus_tag="VFF28_02150"
     CDS             8280..8777
                     /locus_tag="VFF28_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44464.1"
                     /translation="MDIISLLDRLEKDQGFIDWKKKNQFSYLAHVFKLLDDANIDDWQ
                     IGYYNNDDTITTFIITPQEIKVAETENIFKKPDAKINKLDVEKIKIDIAEALQTAEKI
                     QAKEYKNETPFKIITILQNLDIGQVYNITYVTQSFKVLNLKIDSTDGKVLAKSLKSVM
                     DFKAS"
     gene            8866..9300
                     /gene="dut"
                     /locus_tag="VFF28_02155"
     CDS             8866..9300
                     /gene="dut"
                     /locus_tag="VFF28_02155"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013237679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HZX44465.1"
                     /translation="MLDVKIQRIKDNAAIPKYAHPGDAGVDLFSTEEYLLKPGQRVLV
                     STGIKIAVPDGYEAQVRPRSGLALKYGISVCNTPGTVDSGYRGEVGCIVINLGNEDFK
                     IEKGSKIAQMVFNKIEHAEFEEVTELDATKRGEGGFGSTGHK"
     gene            9479..9838
                     /locus_tag="VFF28_02160"
     CDS             9479..9838
                     /locus_tag="VFF28_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44466.1"
                     /translation="MNKKADGGLTAIVIILIALVFLGWIINTGRRECDTNGDCDDAQY
                     CGSDFACHDIPKTIVYKQSLVLPLLFICLTIIALAVIWKWEVIFSKKFEKTVTVEEKT
                     KNKEEPYYTSQFQYTAK"
     gene            complement(9831..10430)
                     /gene="tmk"
                     /locus_tag="VFF28_02165"
     CDS             complement(9831..10430)
                     /gene="tmk"
                     /locus_tag="VFF28_02165"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF013843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HZX44467.1"
                     /translation="MALFIVFDGLDGSGKGEMIKKLEEYLKKKGLNVLVTKEPTDGEY
                     GKQAREILKTDKNPKSAAEKCLSLFVKDREEHLSDIWPFLQKKKGAVISDRYYYSTIA
                     FQHTQGIPMEEVIAENMEFKAPDIAFILDLPAETALERIEKRGNPKEKFEQLSFMKKL
                     RQNFLKLKDELADNIKVIDASKDVDNVFAEIKKEIDNLI"
     gene            complement(10476..10997)
                     /locus_tag="VFF28_02170"
     CDS             complement(10476..10997)
                     /locus_tag="VFF28_02170"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HZX44468.1"
                     /translation="MTEIVVIVAVAQNGAIGKNNDIPWHIKEDFLHFKEKTGKWPVIM
                     GDRTYDSLPEKSRPLPGRENIVCTLDKNYKAAGATVFYDFFIAVDYVKKKGIEKAFII
                     GGATIYKLGMKVADTFELTRIYNDYDADTFYPPIDFSQWKEVNKEDHEGMDLKNKVKV
                     KFSFITYKRIGKT"
     gene            complement(11217..12083)
                     /gene="thyA"
                     /locus_tag="VFF28_02175"
     CDS             complement(11217..12083)
                     /gene="thyA"
                     /locus_tag="VFF28_02175"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020589423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HZX44469.1"
                     /translation="MKAYLDIVKKVLDEGIVRHNRTGTDAITIAGAMFSHEMSSGFPL
                     LTTKSVPFRLVASELEFFLRGISDKKWLQDRDNHIWDEWCSPSIVPYSHDAITKKKML
                     LERDLGPIYGWQWRHFGAKYVDHKTDYTGQGVDQVKRVVDILKKNPNDRQMLVLAWNP
                     MDINKVIPPFCHYGWQATVLDGRLNLMWNQRSVDVALGLPFNIASYGLLLHLLALETG
                     LKEGKLVGFLGDTHVYTNHITGMKEQLSREPMKLPLIKTNNFKSIFDWKYTDSEVTSY
                     EHHPKIKFEIAV"
     gene            12135..12659
                     /locus_tag="VFF28_02180"
     CDS             12135..12659
                     /locus_tag="VFF28_02180"
                     /inference="COORDINATES: protein motif:HMM:NF024605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZX44470.1"
                     /translation="MLIGLTGKMGSGKSEVVKFLKEKGYEHYVYSDVLKEMAKERSIE
                     PTRANLQLLGAKIKGNDPGILSKALLKKARSDNAVFDGIRNRHEIKELKKKGVMIIAI
                     TAPQKVRYQRLKERKRPGDPETFEEFKRLDNLENRGNGKGQEINQCLKVSDHVVDNNS
                     SIENLKENIAKIIR"
     gene            complement(12649..14643)
                     /locus_tag="VFF28_02185"
     CDS             complement(12649..14643)
                     /locus_tag="VFF28_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44471.1"
                     /translation="MKIPASIFSKDKRKAAKPKEDIIEEHAYFSDFEEESQQTVGNDD
                     KYKPKDDIAETTDSFIVTSSEKKVERDLDKKIARLQAEEGREAVNDKEHEPRNAFLEQ
                     RKLDMSEIASGARSDVSEIAYGTYFAPALSMTHQAISEHAQKLITNRQKWGVFDTLAQ
                     SKPAHTFRSAVLDKKKMQKQPGIEHEKEQEQKRRDFLLAERKRELEKQNAAEEKKQLM
                     LEKERQKQHTAEEKKLQKEKEKEQEQMRRSFIIEERKRELEDRKRQRQLMAEKRELDK
                     QNAVEEKKQLLLDKERQKQNIAEEKKLQREKEERKREIEKLKAAEEKKQLMLEKERQK
                     QLIIQQKNLQKEKEKEQKRRNALFEERKRELEKQNAAEEKKQLLLEKERQKQLVAEQK
                     KLQREKESEQEQKRKDFLLAERKKELDEKKRQKQLMAEKRELDKQNAAEEKKQLLLEK
                     ERQKQLVAEQKKLQKEKEKEQEQKRRDFLLAERKRELDRLKAVEERKQLLLEKKRQNR
                     IITEEKKLERDEERKQKLFILEEENKRRLEDIHIKEIEKSIAEATKIITSVKDMAIYA
                     NLKKLFPHKEKPNANEVKKLDITPVFDKPRKKYTPVRFVKNRIYDARDAIGRDDLKTA
                     KQIYSEIMATYLEMNQKDKAKVYAEIKELHDDRKKAESIL"
     gene            complement(14885..15238)
                     /locus_tag="VFF28_02190"
     CDS             complement(14885..15238)
                     /locus_tag="VFF28_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44472.1"
                     /translation="MRPGKFIYWTPRILSIVFILFLSLFSFDVFSPELGFWQIALALL
                     IHNIPALFLLAVLLISWRYEIFGGIVFILAGLVYIALVLMNPFKWYMISWFLIIALPA
                     FFIGALFLIGWYNKR"
     gene            complement(15538..15765)
                     /locus_tag="VFF28_02195"
     CDS             complement(15538..15765)
                     /locus_tag="VFF28_02195"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HZX44473.1"
                     /translation="MIKEQRFSIFVKPNSKKNEIIGYDASRKAYVIRIAARPEDNKAN
                     LEAIKFLSKTLGKKARIVSGLTSKTKVISTE"
     gene            complement(15762..16058)
                     /locus_tag="VFF28_02200"
     CDS             complement(15762..16058)
                     /locus_tag="VFF28_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013180455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor S"
                     /protein_id="HZX44474.1"
                     /translation="MMPTKEGEKTILVCRSCGAKSADKDGGKIKETSKEETSKVEVIE
                     NKDAFETLPKMKVECPKCHNSEAFFWEVQTRASDEPATKFMKCTKCAHIWRDYN"
     gene            complement(16082..16708)
                     /locus_tag="VFF28_02205"
     CDS             complement(16082..16708)
                     /locus_tag="VFF28_02205"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZX44475.1"
                     /translation="MDRPELKKKMKKLWWFIWEDNSIWSWLVNIVLAFVLIKFLVYPG
                     LGLLFGTGYPIVAVVSGSMEHDGSFNKWWNTQADWYTENGIAKDEFSDFSFKNGFNKG
                     DIMILYGKKAANVNVGDVIVFKSARQNPRPDPIIHRVVKKWEKDGQTYYQTKGDHNPD
                     SINGCSQNECLFEGDIREDQYLGNALVRIPFLGYIKIWFVELLGLFVR"
     gene            16763..17083
                     /locus_tag="VFF28_02210"
     CDS             16763..17083
                     /locus_tag="VFF28_02210"
                     /inference="COORDINATES: protein motif:HMM:NF016842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NMD3-related protein"
                     /protein_id="HZX44476.1"
                     /translation="MRQDYFEGVLQLRNPSKELMSFVNNQLKKNSDKVWVAKHELQDS
                     GIDMYISSNKFLQSLGKKLKKTFKGELIVSGRLHSRDRQTSKDKYRMTVLFRMHSETP
                     DSQR"
     gene            complement(17046..18233)
                     /locus_tag="VFF28_02215"
     CDS             complement(17046..18233)
                     /locus_tag="VFF28_02215"
                     /inference="COORDINATES: protein motif:HMM:NF016219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF460 domain-containing protein"
                     /protein_id="HZX44477.1"
                     /translation="MGEKKLLIVGVDPGTTLGYGAIDFEGKVVAVHSDKNLDINILIS
                     ELIKIGKPVIIASDKEHNPDFVNKTAVKLGARLISPDYDLKVVEKRRITSEIETRNQH
                     EIDALASAFFALRKLQPLLNKINIFVEHYKKEAIKSGLIELVVGKGLNIRDAVEILEE
                     PEHKEERIIKEVVAEKKLDENDFLTLYKKYKSAKKDIYLLREHNKRLNEESEKLKKDY
                     EYMLKRISKANGDKKTESCLEFKERRIHILDKQLRERDYQIQAMQNEITTLLYFLANI
                     NDNVLLKKLDNLGSVELEKKKALLGVSEGDILLVKDADIYSINAVNEIKGKVGIIVYK
                     KPISKKVEKSLPFFFIDASKLEIDENEYFAITSKESFEKAKGHKGLLDKVISDYQEFR
                     NAS"
     gene            18337..18969
                     /locus_tag="VFF28_02220"
     CDS             18337..18969
                     /locus_tag="VFF28_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF120 domain-containing protein"
                     /protein_id="HZX44478.1"
                     /translation="MGKHFGFLVLIAEKTGQFGMLKTSTLSLSKELGVPQQTISRKLK
                     DMERIGLIKRQVSPKGIILSIKDEGRKLLQKNYEKLKEILGRENPITGTVEKGIGEGS
                     YYVSQKGYQSQFKEKLGFIPFQGTLNVKVGEEALAQLLSKPQITIDGFYGGNRTFGSI
                     SCYKIKISSIDAVIVRPERASHPSDILEIIAPINLRNALKLKDKSKVKLK"
     gene            18966..19430
                     /gene="ribC"
                     /locus_tag="VFF28_02225"
     CDS             18966..19430
                     /gene="ribC"
                     /locus_tag="VFF28_02225"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013683600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HZX44479.1"
                     /translation="MKVGIADTMFARQDMFKIVKKALEDSMEPGIRLERYTVPGVKDL
                     PVACKKLFEEYGCDICMALGMPGDKPIDKVCAHEASNGLIHVQLMTNKHIIEVFVHLD
                     EGKDEKELLEIVHNRVYDHTLNTLALLKGKEALSKKAGKGIRQGKESVGELR"
     gene            19435..>19680
                     /gene="ribH"
                     /locus_tag="VFF28_02230"
     CDS             19435..>19680
                     /gene="ribH"
                     /locus_tag="VFF28_02230"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015791115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HZX44480.1"
                     /translation="MANEKLGFVVSDFNADITHLMSKLAQEHAEFLQAKVAKVYRVPG
                     AFDMPLAVSKLVNMQDIDGVVCLGAVIEGDTQHDEIVA"
BASE COUNT         5728 a         4368 c         3697 g         5887 t
ORIGIN      
        1 cagacaggaa cctctacagc aatcgttgag gtgtcgtttg ccagcataaa atcctggatt
       61 atgccgtgcc tctccctgtt tgtctttgcc atctttaaag caagcccgga gagctcacaa
      121 gcataattct tcttttttac aaccctggcc ttagaatcat taaccttaat cctgcttcct
      181 cttgcgttat cctccaaaaa catctcatgc gggcaaagcg actgcacatt gaggagatat
      241 tcctttaagc caagatagta ttctgtcagg aacatgccta tcttcatctt atggcattta
      301 tagagataag gctgcttgtg gtcaaatagc ttttccaaaa caggtgaaga aaaccctgac
      361 tttctgcctt ctctaacctt gccccttatc cttctaaaag tgcatatctt agaaaattct
      421 ttcagccacg tgcttatcag ctttggataa gtctttacat ggaacctctt gtttatctcc
      481 aaagacgctt gctcaagagt catgcctttg ttgtaaagcg atattgcgct aagtattatt
      541 tttgcagggt aagatttatt gagcattttc ttgcctgtaa aataccttcc gcaccccata
      601 cagcaatact tctgcacttg ccccatcttg ttttttctat agcccttttt tagtatatcc
      661 ctgctttcgc aatgtctgca ggttatgctc tcacttggca taatatcctc ctttccttgg
      721 gcttccccta aactcgattt ttccatcgtc ttttagtttc ctaagctgcc tttcaagcgt
      781 cctcttggcc attcccatct ccttggtcat ctgcgatgtt ctttttcctg gatttttccc
      841 aacaaattca agaagattta caccgccatt tacaccgcca tttacaccgc catttacacc
      901 gccatttaca ccgccattta caccgccata tgtgtttcca tcaagaagcg ttactgcaaa
      961 tgaggtatta aaatcaaaaa taggctctgg cagtccagcc cttttcattg aatcccttat
     1021 tctgtttatt cctgttccaa ctttctcgac ataatcggtt tttgacagga gttcggcgat
     1081 gatctggttt cttgccctgc tgtatcttcc aaagtcctca ggcttaagcc atttcacaag
     1141 cccgccaggg tttgatattg ctattttgtt cctaaaaact tctatcagaa catcaccaga
     1201 cctatcaaaa tagtccctat gcataatggc attgacgata gcttccctaa acgcttcctc
     1261 gggaaactgc agaatctcat tccttagcgc cgtttttatt tcaaatctca cattgatatg
     1321 cttcttgaca taattgattg cttccttgat gttatccatg attccgttgt caaatatctt
     1381 cttgtcaagg atattcacct tctcgttggt ctggtaattg acacagacaa cctttgagtt
     1441 gataaagaat atctcaggac ttttggcaaa aaatagcact ccggcgttag tcatatggat
     1501 cttattttta actggctttg cagcctttaa agaaaccaat atatcttccg gaggtgtatt
     1561 ttttgtaagg cgggataaag aaagataagc atcaagtttt cttgcgtcaa aatcttcagg
     1621 gaacctgaac tcggggttta tttgctcatc gaatttaatc tttccttcac ctactgaaaa
     1681 ctctattatc tcatctctag tcagcttttg ggaattggcg ccggtcctaa gatagaatcc
     1741 agaggagcac tgataaggct tgttttttcc ttccagcaca tgaataatca gtatacctct
     1801 atattcttct atccagatgc tggctgcagg gtcgcagttt cttgcgatat cctggatttc
     1861 tgactttagc ctattgctta ttgtaatgcc tttaacttcc atcctgtcat taattccaat
     1921 gaatatctgt ccgccctcgg aattggcaaa agcgcagatt tcctttgata ggttttttgg
     1981 gtcaaagctt tccttgaatt caataaactg gccttcgcct tcttctagga ttaaattaaa
     2041 ttcggattca tccatttgca agcatcctcc ttatctcatc gagcctcatc ttcttttgat
     2101 aggcgtcaag cgggaccaaa gtgccattga tcattataga atcatttact atccgagaat
     2161 ctcttgcata gattgtgata tccctctcag cagtaagatt ctcctcaaaa gtcacacgaa
     2221 ggtaatcacc agaaggaata agagtccaat tatcatcctg tactttaaca taatcataaa
     2281 catcatcaac aaatgcccta ttttcatcca gatgttctgc cttagttatc tctatcacca
     2341 gctcatacgt ctggttggaa agagaaggta ctacccactc gatataatca gtaagcgaat
     2401 tattgttatt atcatacctg tcgaaataag cctcttcccg gatttcattg acaagcctat
     2461 aaagcctaat agatattgtt tcaggagtga catttgtaaa ggcagttata ttctcatagt
     2521 gcacatcaga cgacaccact atctgcttta cagtagcgct aagcacaatc tctgtgctta
     2581 taggtgcttc tgtataatat tcgactgtaa cattagaaac aggctcttct attatgacct
     2641 ctatgtcctt tggcttgtcc ttgtcatcct tatccttctt gtctttgtcc tcattttcct
     2701 tgactgcata gccggttata tctatattaa gcagccattc aaagaagcga gtaaataatc
     2761 cttctgcaga agaaacaaca gcgtgtccag tcagcttgtt cttttcactt gaaagctcgc
     2821 taagttcctt gttgatagaa gctatctcgc cagcagcagt aggcctctct tggattatct
     2881 cttctttctt ggcaatcagc tttgttgtca tcttctctat ctgccctatc ttcttttcag
     2941 cgataaaggt tgaagcttcc ttctctacac catcgacaac aacagttacc ttctcatcag
     3001 aaagacgctc atcctcagta tacacgctca cattaagggc atcagatgat atgttcaagc
     3061 tcaggttgga aacatatgaa gaagtgtcca ctctctttac ccacctaaca ggctcattta
     3121 tccttatctc aggattgact tcagagatag tgacattggc aaaagcagca gccactggcc
     3181 tatcgatcac ctttatctca aatatattgc tagtggtggt ataatatcca tcagaagcgc
     3241 tgaaatacac atacctcact cctgtaaagt tagcaggcag gacaaccgcc acatcaccat
     3301 atacagaaat gctgacattc tcaacagcat tgctataaaa gagagttata tcattatctt
     3361 catcagagaa gtaagcagac aggttaagta aaacattctc tcctttgatg atagtaatat
     3421 tctcaaaagg ttttactaag acaggcatat ggccagggat ctcttcctcc aaaacataat
     3481 gtatgctgtc aatatccaag ctgccgttct ctatctccac tctaagcaca tactcagaat
     3541 agttcagcga aggcagcagg caggtttcca aacattcaga agtaaaagag acaaaaggct
     3601 caaacacagc atatgcaagc atctcgtcag aataggatac atcagagtaa gcattggaag
     3661 ggtcaagcga atagttggca aacaccataa cagcccgcac ttcattctca tacgagcttc
     3721 catacctttc ataggttaga tacaagctgt cgttccattc tcctgaagag ctaaggccca
     3781 gaagtgcaca gcaatcaaaa tcaccgttgc acatctcata actgttgacg ctccatctca
     3841 cacagacttt ctcgccagat ctattgctag aaacatcaaa cctaaatatc ccatcgagtg
     3901 gtttgctccc tcctccattc aaaggaatag ttgcatatcc tgataatagt ccttcctcac
     3961 tattatcata cacaagatag cttttattgc ctgcatcaag atatactctt gccttgcctg
     4021 ctatttggcc gtcgatgctg agtcccttta gtattccatc gataaacaaa ggatattccg
     4081 cagaagagat tgcactgaaa ttaatctcct ttgtcacgct tatgtctgag gtataaacag
     4141 caagccctgt atatttgtcg gcgataaata agcctaaggc aaacagcacg agtaccaagg
     4201 caaatattcc ttttccccta tacaaggcag cagtcatggt ttccaccata tccaggttcc
     4261 tttgccagaa cccactgcct tgcctttgat tagcccttca ccgcgaagat tattaaggcg
     4321 gtaaagggct tttactcttg aaatcccttt tagaagaagt tctatctccc ttgtctctag
     4381 aggctcgcca gcgtttgtta ttatatcaag tatctttttg ctagtcccat ctagttccct
     4441 gcgcattaac aagtgatttt gtaacaatgg tttataaagt tatcgataaa tatattgata
     4501 taattatgga cacattcatc ggtaaacaaa acccatccat aaggttttta tatagcttca
     4561 tgcaaagctc aacagatgaa gcaaatcctg gcagaaatat taaaagacat caagccaagc
     4621 agcaaagagg aaaaagcagt aaaagacaaa gcagatgctg tcataaagac cataaactca
     4681 ggcctacggg acgcaaaagc tatattaggc ggctcgggga taaaaggcac atggcttagg
     4741 caggcaaatg atatcgacat tttcgtaaaa tacaactacc aaaaatacaa ggaaaaaagt
     4801 ggccagcttt catctttgct ggaaagccat ctaaaaaaga aaggaaaaaa gattgacaag
     4861 cttcatggca gcagggatta ttttcagata aaagaagatg gctatacgat tgaggtagta
     4921 cccatccttg acataaaaga tgcaaaagcg gcaaaaaaca taacagatat ctccccgctt
     4981 cacgcaatat gggtaaataa aaaaggaaaa aacctcaaag acgacatccg cctcctgaag
     5041 caattctgca aagcttcaaa gatatatggc gcagaaagct atattcaggg cttctcagga
     5101 tatatctgtg aaatcctgat aatctactac aaaggctttg agaatctgat aaaggcagcc
     5161 gcgaagtgga aagataatat taccatcgac gtagaaaacc attggaagaa aaagaacata
     5221 cttctggaac tcaacagatc aaagataagc tcgcctttga taattatcga tcctgtgcaa
     5281 aaagaaagga atgcagcagc ggctgtcagc aaagaaaatg ttggcatctt taaaagaaga
     5341 gcaaaagaat ttctaaaaaa accttcaact gatttcttca agatacaacc attagataaa
     5401 ggatccctaa agaagagggc aaaagacaac atattgataa tgcttgaagc agtcccaaaa
     5461 aaaggcaagg tggatgttgt cggctgcaag atagtaaaag cctttgagca cataaaagca
     5521 gagtttgcaa aaaatgattt caaagtgtta tcccaaggtt gggaatggaa caagactgcc
     5581 tttatttatt ttataattaa aaaacaacag ctgtcagatt acaccgaaag agaaggcccg
     5641 ccattatctc tcaagttcca tgctgataag tttaagaaag cccataaaga cactttcata
     5701 aaaaacaaca ggctctatgc aaagatgcag cggcaattta aaaaaccaga ggatcttata
     5761 gagagcttga agggttctca gtacataaaa gagcaggtcc aagagttaag atggaaagga
     5821 taaggcttga gataaaaaga cagcccattc atatgatgct ggggtttatc ctcattgcac
     5881 tgataaaata tgacctccta agtcccatac cttttgcgat aataatactt actgcgatag
     5941 gttcatccct ctacctgaag aaaaaaaagc caaaaaggat ttacaacttg ctaagatatg
     6001 tagaaaggcc tgatgccctt gagaactttc caggaaaggg gctcatcacc tacctgatag
     6061 gagcgctcat tgcaatagtc ctctttgaaa aaagtacagc aatggcagcc ataatcattc
     6121 ttgccctcgg cgattcggtg tcaaggcttg taggcccgtt tggaaggata aaacatccat
     6181 tcaacgacgc aagattcctt gaaggagtga ttgcaggatt tatcgcagga gcccttggag
     6241 cgatgatctt tgtaagccca tctcaggcca taaccgcatc ctttgttgct atgataatag
     6301 aaggtataga cttaaagctg tttggcataa aagtagatga taatattatc atcccatctc
     6361 ttgcaggagc agctgtctgg ctgatgggaa ttatagcatt ataacatcag tatgtattaa
     6421 gctaaccagg gggactctac tgtcctaagg tcaaaactcc atccaataac aattatacag
     6481 ctcgtcgtag ggggtcgccc cagccgggga tacagagcct ttgagaaaca aataaaaaat
     6541 cccaaccgca gcaagctgcg gggtattttt taagcctcaa actgggttat taagccgtag
     6601 caagctgcgg ggtatgaacc caagtcttga gcaatcaaga gttttgaaga caatagagcc
     6661 cccgtcaaga gcttaataca tactaacatc aacactcttt aaatataagt agctaacaat
     6721 caataaaaca tgaagctcct cgcttttgtt gaccttcacg gcaaccaaaa agccctaaaa
     6781 gatattcaga aaaaatcaag gcatgcagac atgatagtct gcgcaggaga tatatcccta
     6841 tttgaaaaca gccttgacag cctccttctt cagctggagt tgttgaaaaa accagtgatt
     6901 atcatacccg gcaatcatga ggacgaacat gacctaaggc tggcctcaag cgtctttgga
     6961 aatatccatg acatcaacaa caggtcatac atgatagggg attacctatt cctgggttat
     7021 ggtggtggcg gtttttgtag ggttgacaaa aagtttgata agatagccaa aaaattcaca
     7081 ttgataataa agaaaaacaa gggaaaaaag ataatacttg tgacgcatgc ccctccctac
     7141 aggacaaatc tggataacat cgaaggcgat tcctgcggaa acaaggccat aagaaatttc
     7201 atcgacaaag taaagccaga ccttgtgatt tcaggccatc ttcatgagaa cgcaggaaaa
     7261 ggagacaaaa taaaaaacac ccggctgata aacccgggcc cctttggaaa aataatagtc
     7321 atctagaaag caggacaacc ctgctgtctt tcttttcatc ctttatctta tagccagagg
     7381 caagtgcaat ctgcgctgca aactcagcta tctcagaatg caggggcatg ttctctatct
     7441 taagcctttt ttgtgaatac ccgacgtgca tatatgcctt cacttcgata tattttggcc
     7501 tatatttctt tacaagtgct ccatattgct caggacactt catgtttact cccttaacaa
     7561 gggtcagcct gactacgctt ctcttaaact ttgaaagcaa agacagcgat ttaagtattt
     7621 tagcccatcc attcttgata agaggcttgc acactttaag ataggtttct tcatcaggcg
     7681 caggtaaagt gatataaatc tgggtaggct gtgccgcaat cagcttcttt atcatagaag
     7741 ggtttgtccc atttgtcaca agaaactggt ttatcttcct tttgcgcaat tcaagtataa
     7801 gttcaggaag cttaggatac atggtaggct cgccagataa agagattgca aactgttgag
     7861 gatttgagag ctcgccgaat tttttcctgt tcgttttatc atttcctcca aacccttgca
     7921 ggtacttcgc attggcagct atgcattcat cgacaatctc tgaaggatca tcctcttttc
     7981 ctttccattt ggggagtgtg aactctatgt ctctccagca ccagacgcac ctatggccgc
     8041 aaaatccaag cgcaggcgtc atctgcacac acctatggct caaagtatca tagaactcct
     8101 ctttatagca atgtcctgag tcaagaagcg actttttgca ccaaaggcag ggctttactg
     8161 cactatgctt accgacaatc ctatagccct gctggtaaag gttttctttc ttgctttcaa
     8221 cgatcatgac ataagaaaaa tacaaaagtt atttaaatta attcctttta gcgttcttta
     8281 tggatattat ttctttgctt gataggcttg aaaaagacca aggcttcatc gattggaaaa
     8341 aaaagaacca gttctcatat cttgcgcatg ttttcaagct gcttgatgat gcaaacatag
     8401 acgattggca gataggctat tacaacaacg atgatactat aactactttc ataatcacac
     8461 cgcaggagat aaaggttgca gagacagaaa acatattcaa aaaacccgat gccaagataa
     8521 acaagctaga cgtagaaaag ataaagatag atatcgccga agctctgcag acagcagaaa
     8581 agattcaggc aaaagagtat aaaaacgaga ctccatttaa gataataacc atccttcaga
     8641 acttagacat cggccaggta tacaatatca cctatgtcac tcaaagtttc aaggtcctaa
     8701 atctcaagat cgactcaact gacggaaagg ttcttgcaaa aagtctgaag tcagttatgg
     8761 actttaaggc ctcatagcac ttcgtaactg cataagtata acgctttcta aactatattt
     8821 tgcaaactga aattctttat aaataagtaa atgggaaaat aatccatgct cgatgtaaaa
     8881 attcagcgga taaaagataa cgcggcaata cccaaatatg cacatccagg tgatgctggc
     8941 gttgacttgt tctctacaga ggaatacctg ctaaagccag ggcagcgtgt gcttgtatcg
     9001 acaggcataa agatagcagt ccctgatggc tatgaagctc aggtaaggcc aaggtcaggt
     9061 cttgcattaa agtatgggat aagcgtctgc aatactcccg ggactgtgga ctctggttat
     9121 aggggagagg ttggatgcat cgtgatcaat ctcggaaacg aagacttcaa gatagagaag
     9181 ggctcaaaga ttgcccagat ggtcttcaac aagatcgaac atgcagaatt tgaagaagtg
     9241 acagagcttg acgcaacaaa aagaggagaa ggaggctttg gctcgacagg gcacaaataa
     9301 catggcatac caaaaaccaa agataagcta caacacagta aggaagaaca caagtctggg
     9361 caagttccta aaagccgagg aagaggaaga agaggaagca aaagataatt cttaatcatt
     9421 agtaagtagc aaaacattta tatagcgcaa aagcatcctg cccatatagg gtggtcaagt
     9481 gaacaagaag gcagatggcg gtctaacagc tattgttatc atactcatag ctcttgtatt
     9541 tctaggctgg attataaata ccggaaggcg tgaatgcgat acaaacggtg attgtgatga
     9601 tgcccaatac tgcggctctg actttgcatg ccatgacatt ccaaagacaa tagtctacaa
     9661 gcaaagcctt gtccttccgc tgttgttcat atgcctgact ataattgccc tggcagttat
     9721 ctggaaatgg gaagtgatat ttagtaaaaa gtttgaaaaa acagtcacag ttgaagaaaa
     9781 gacgaaaaat aaagaagagc catattacac aagccaattc cagtacacag ctaaataagg
     9841 ttgtctatct cttttttaat ttcagcaaac acgttgtcaa catccttaga agcatctatc
     9901 accttgatat tgtcagcaag ctcatcctta agtttaagga aattctgcct aagctttttc
     9961 ataaaggata gctgctcaaa cttctccttt ggattgcccc ttttttctat cctctcaagc
    10021 gcagtctctg caggcaggtc caggatgaac gctatgtcag gagctttgaa ctccatgttc
    10081 tcagcgatca cttcctccat ggggattccc tgggtgtgtt ggaatgcgat agttgaataa
    10141 taatatctgt cagagattac agcacctttt ttcttctgca ggaatggcca gatgtcagac
    10201 agatgctctt ctctgtcctt cacaaagagg ctaaggcatt tttccgcagc tgattttggg
    10261 ttcttgtcgg ttttaagtat ctcccttgct tgttttccat attcgccgtc tgtgggctct
    10321 ttggtgacaa gcacattcag ccctttcttc tttagatatt cttccagctt cttgatcatc
    10381 tctccttttc cagagccatc aaggccgtca aaaacgataa ataatgccat aaaaaaacta
    10441 aaagaaggta tctatattaa ccttgcgtta tatcatcatg tttttccaat tcttttgtaa
    10501 gtgataaacg aaaacttgac ctttacctta ttcttaaggt ccattccttc atggtcttct
    10561 ttatttacct ctttccattg ggaaaagtcg ataggtggat agaaagtatc tgcatcataa
    10621 tcattgtata tcctggtcag ttcaaaggta tctgccactt tcatcccgag cttatagatt
    10681 gtagccccac cgatgataaa cgctttctca atcccttttt tcttgacata gtcgacagct
    10741 ataaaaaaat cataaaacac ggtcgccccg gctgccttat agttcttatc aagcgtgcag
    10801 actatatttt cccttccagg cagaggccgt gatttttcag gcagcgaatc atatgtcctg
    10861 tctcccatga ttacaggcca cttgccagtc ttttccttaa aatgcaggaa atcctccttg
    10921 atatgccagg ggatatcatt attcttacca atagctccgt tctgtgccac tgcaacgata
    10981 acaacgattt ctgtcatatt aaacctcaag ggaaaaaatg acaacctggg cgaaaactgc
    11041 ctaaaagagg aaaaaaccat gcgaagtatc ggttttttcc ttttggcaaa cctcaatctt
    11101 tgcatagcaa agttgaagac ttacaaatca tagggatttg aaagtggacg tgcaaagcac
    11161 ggccgtgttt gagccctttg ttgttactat tttttccagt tattgattaa caaatcctaa
    11221 acagcaatct caaactttat cttcggatga tgctcatagg aggtaacctc tgagtcagta
    11281 tacttccaat cgaatatcga tttaaagtta ttggttttga taagaggcag cttcattggc
    11341 tctcttgaaa gctgctcttt catcccagtg atatgattag tatatacatg tgtatctccc
    11401 aaaaatccca caagctttcc ttctttaagt ccagtctcaa gcgcaagaag atgaagtagc
    11461 agcccataag aagcgatatt aaatggcagg ccaagagcga catccacgct tctttggttc
    11521 cacatcaggt tcaatcttcc atcaagcaca gtggcctgcc agccataatg gcagaatgga
    11581 ggtatcacct tgtttatatc cataggattc caggcaagca caagcatctg cctgtcgttt
    11641 ggatttttct ttaggatatc gacaaccctc ttgacttgat cgaccccttg ccctgtataa
    11701 tcagttttat gatcaacata cttggctcca aaatgcctcc actgccagcc atatatcggc
    11761 ccaaggtctc tttcaagaag catcttcttc tttgtaatgg catcatggct gtagggaact
    11821 attgaagggc tgcaccactc atcccagata tggttgtccc tgtcctgcag ccatttcttg
    11881 tcagatatcc cccttaaaaa gaactcaagc tcgcttgcga ccaagcgaaa agggacagat
    11941 ttggtggtta gtaaaggaaa accagaagac atctcatgtg aaaacatggc gcctgcgata
    12001 gtgattgcat cagtgcctgt ccgattatgc ctgacaatac cttcgtccaa gacctttttt
    12061 actatatcaa gataagcttt catgactccc ccaaaataaa catctttaaa taatatttaa
    12121 tacttttcat ttctatgctg atagggctta ctggaaagat gggttcagga aaaagcgagg
    12181 tagtaaagtt cctaaaggaa aaaggctatg agcactatgt gtattctgac gttttaaaag
    12241 agatggcaaa agaaagaagc atcgagccga caagagcaaa tctccagctc cttggagcca
    12301 agataaaggg aaatgatcca ggcatacttt caaaagcgct cctaaagaaa gcaagatcag
    12361 ataacgctgt ctttgacggt atcagaaaca ggcatgagat aaaagagctg aaaaagaaag
    12421 gcgtgatgat aatagcaata acagccccgc aaaaagtaag ataccaaagg ctaaaagaaa
    12481 gaaagcggcc gggtgatcca gagaccttcg aagagttcaa gcgccttgac aacctcgaaa
    12541 ataggggtaa tggcaagggc caggagataa accagtgcct aaaggtctca gatcatgtgg
    12601 tagataacaa ttcatcgata gaaaacttaa aggagaacat tgcaaagatt ataagataga
    12661 ttctgccttc ttcctgtcgt catgtagctc ttttatttca gcatagacct tagccttatc
    12721 tttttggttc atctcaagat aggtagccat tatctcagaa tatatctgct tggcagtctt
    12781 aaggtcatct ctccctatgg catcccttgc atcatatatc ctgttcttga cgaatcgtac
    12841 aggagtgtat ttctttcttg gcttgtcaaa gacaggagtg atatctagct tcttgacctc
    12901 attggcatta ggcttctcct tatgcgggaa aagcttcttt aggtttgcat agatagccat
    12961 atccttgact gaggttataa tcttcgtcgc ttccgcaatc gacttttcta tctcttttat
    13021 gtgtatatcc tcaagccttc tcttattctc ttcttcaagt ataaatagct tttgttttct
    13081 ctcctcatct ctttccagct ttttctcctc agttataatc cgattctgcc tcttcttctc
    13141 aagcaaaagc tgctttcttt cttcaactgc ctttagtctg tcaagctctc ttttcctttc
    13201 agctaaaagg aaatctctcc gtttttgttc ctgctctttt tccttctctt tctgcagctt
    13261 cttttgttct gccacaagct gtttttgcct ctccttctca agtaaaagct gctttttttc
    13321 ttcagctgcg ttttgtttat ccagctctct cttctcagcc ataagctgct tttgcctttt
    13381 cttctcatca agctctttct ttctttccgc taaaaggaaa tccttccttt tttgctcctg
    13441 ctcgctctcc ttctctctct gcagcttctt ttgttcggcc acaagctgtt tttgcctctc
    13501 cttctcaagt aaaagctgtt ttttttcttc agctgcgttt tgtttttcaa gctctctttt
    13561 cctttcctca aaaagggcat ttctcctttt ttgttctttc tccttttctt tctgaagatt
    13621 cttctgctgg attataagct gtttctgtct ctccttttca agcataagct gttttttttc
    13681 ttcagctgcc tttagctttt caatctctct tttcctttcc tctttctctc tctgcagctt
    13741 tttttcttca gctatattct gcttctgtct ctccttatca agtaaaagct gctttttttc
    13801 ttcaactgcg ttttgtttat ccagctctct cttctcagcc ataagctgtc tttgcctttt
    13861 tctatcctca agctctcgtt tcctttcctc tatgatgaag cttctcctca tttgttcttg
    13921 ttctttctcc ttctctttct gcagcttttt ctcttcagct gtatgctgct tctgtctttc
    13981 cttttcaagc ataagctgct ttttttcttc agctgcgttt tgtttttcaa gctctctttt
    14041 cctttccgct aaaaggaaat ctctccgttt ttgttcctgt tccttctcat gctcaattcc
    14101 aggctgcttc tgcatctttt tcttgtcaag aaccgccgac ctaaaggtgt gggctggctt
    14161 actctgggct aaagtgtcaa aaactcccca tttttgcctg tttgtaatga gtttttgagc
    14221 atgctcagaa atcgcttgat gtgtcataga caaagcagga gcaaagtatg tgccataggc
    14281 gatttctgac acatcagagc gtgcgcccga agcgatttct gacatgtcaa gcttcctttg
    14341 ctctaaaaag gcatttctcg gttcatgttc tttgtcattt actgcctctc ttccttcctc
    14401 agcttgtagc cttgctatct tcttgtccag atctctttct accttttttt cgctggatgt
    14461 aacaatgaat gaatcagttg tttccgcaat gtcatcttta ggcttatatt tgtcatcatt
    14521 tcccacagtc tgttgactct cttcctcaaa atcgctgaaa tacgcatgtt cttcaataat
    14581 gtcttctttg ggtttagcag cctttcgctt gtctttagaa aaaatgcttg caggtatttt
    14641 cactgcagca ggcttaacct ttttttggat aagtttagta acaaacccaa atagtggttt
    14701 ctttagcttc tcttcgtgcg cttcttcagc ctcattcagt ttgctaagcc tatcaagaat
    14761 ttcaagaggt gggggtggtg gcagttcatc tagctcatct atcccgctag atttaaggct
    14821 atggctcttg attgctgact cacccctttt ttttattgat tattagattg cttgtttata
    14881 aaacttacct cttattatac cagcctataa ggaataaagc accgataaaa aaagcaggca
    14941 aagctatgat taaaaaccat gatatcatat accatttaaa tggattcatt agaactaaag
    15001 ctatgtatac taggcctgct aggatgaaaa ctatccctcc aaatatctca tacctccatg
    15061 atattagaag aactgcaagc agaaaaagcg cagggatatt atggattagc aatgcaagcg
    15121 ctatctgcca aaatcccagc tcaggggaaa agacatcaaa ggaaaaaagc gataaaaaca
    15181 atatgaaaac gatagatagt atccttggcg tccaatagat gaacttgcct ggcctcatgg
    15241 accattatag cgcctgcttg tataaataga tgcctaattt aatatattac cccatacatt
    15301 cggattatat gcatgagata aacatccagc aactgattgc aaaagcagaa gatttcaagc
    15361 aaaaggggat accttggcac caccatctat taacaccaaa ttgctgcttt aactcaagcg
    15421 gtaaatatag aataatcttg gaaaacgaaa ttacaaaaga agctttctac tgcgatttca
    15481 cagataaacc gatgactcac ttaaagcgat tagaagaact attttttaaa tcattagcta
    15541 ctcagtactg attaccttcg tcttgctcgt cagcccagaa acgatcctgg cctttttccc
    15601 gagggttttg gaaagaaact ttatcgcctc aagatttgcc ttgttatcct caggcctggc
    15661 agcaatcctt ataacataag cctttctgga agcatcatag ccaattattt catttttctt
    15721 ggaattaggc ttgacaaaga tagaaaacct ttgttctttg atcaattata atctctccag
    15781 atatgcgcgc actttgtgca cttcatgaac tttgtagcag gctcatcaga ggctcttgtc
    15841 tgcacttccc aaaaaaaagc ctcgctgtta tggcattttg ggcattccac tttcattttt
    15901 ggcaatgtct caaaagcatc tttgttctca atgacctcga ccttgcttgt ctcttccttg
    15961 gaagtctctt ttatcttacc gccgtcttta tctgctgatt tagccccgca gctcctgcag
    16021 acaagtattg tcttttcccc ttcctttgtg ggcatcatta ttgccccgca tttacaaaac
    16081 atcatcttac aaacaacccc agtagctcga caaaccatat tttgatatag cccaggaaag
    16141 gtatccggac caaagcattt ccaaggtatt ggtcctccct gatatctcct tcaaacaagc
    16201 actcattttg gctgcatccg ttgatagaat cagggttatg gtctcccttt gtctgataat
    16261 aggtctgtcc gtccttttcc cactttttga cgactctatg gattataggg tcaggcctgg
    16321 gattttgcct tgcactctta aatactatta catctcctac attgacattc gcggccttct
    16381 tgccgtatag gatcatgatg tctcccttgt taaatccgtt cttgaatgaa aaatcagaaa
    16441 attcatcttt ggctatccca ttctcggtat accaatctgc ctgagtgttc caccatttgt
    16501 tgaagctgcc gtcatgctcc atagagccag agaccacagc aactatcggg taccctgttc
    16561 caaacaaaag cccaagtcct ggatatacaa ggaatttgat aagcacgaaa gcaagcacta
    16621 tatttacaag ccagctccat atggagttgt cttcccatat gaaccaccac agttttttca
    16681 tttttttctt aagctcaggc ctatccatca aggaggaaat gttgggaaag tattaaaaag
    16741 ttatcgtttt tatcagtagt atatgcgcca ggattatttt gaaggagttc tgcagttgag
    16801 aaacccaagc aaagaactga tgagctttgt caacaatcag ttgaaaaaaa acagtgataa
    16861 ggtctgggtg gcaaagcacg agcttcaaga cagcggcata gatatgtaca tttcctcaaa
    16921 caagttcctg cagtccctcg gaaaaaagct gaagaagacc ttcaaaggtg agcttatagt
    16981 cagcggaagg ctccacagcc gtgataggca gacaagcaaa gataagtata ggatgactgt
    17041 gctcttcagg atgcattccg aaactcctga tagtcagaga tgaccttgtc tagaagccct
    17101 ttatgtccct tggctttctc aaaactttcc ttggaagtta tggcaaagta ttcattttca
    17161 tctatctcta actttgaagc atcgatgaag aagaatggaa ggctcttctc tacctttttg
    17221 cttatcggct ttttatagac tattattccg accttgcctt tgatttcgtt gaccgcattg
    17281 atagaataga tatctgcatc tttaacaagc aggatatccc cttcagatac gccaagaagc
    17341 gctttctttt tttcaagctc tacgcttcca aggttatcta gttttttaag aagaacatta
    17401 tcgttgatgt tcgccaagaa ataaagcaag gtagttatct cattttgcat ggcctgtatc
    17461 tgataatccc tctctctaag ctgcttgtca agtatatgta tgcgcctttc cttgaattca
    17521 aggcacgact cggtcttttt atcgccgttt gccttgctta tccttttaag catatattca
    17581 taatctttct ttagcttctc tgactcctca tttagccgtt tgttatgctc tctaagaaga
    17641 tagatgtcct tttttgcgct cttatatttt ttatacagcg taagaaaatc attctcatca
    17701 agctttttct cagccacaac ttccttgatt atcctttctt ccttgtgttc aggctcttca
    17761 agtatctcta cggcgtccct tatgtttagt cccttgccca cgactagctc gataagcccg
    17821 ctcttgatag cttctttttt ataatgctcg acaaatatat ttatcttgtt gagcaagggt
    17881 tgaagcttcc taagtgcaaa aaaagcagag gcaagcgcat ctatctcatg ctggtttctt
    17941 gtttcaattt cagatgttat ccgcctcttc tcgacaactt taaggtcgta atcaggtgag
    18001 ataagcctgg cgccaagttt tacagctgtc ttgttcacaa aatccggatt atgttctttg
    18061 tcgctcgcga ttatcactgg ctttcctatc tttatcagtt ctgagataag gatattgatg
    18121 tccagatttt tatcagaatg cacagcaaca actttgccct caaaatctat agcaccatat
    18181 ccaagtgttg tcccagggtc gactccgact attagtagtt tcttttcccc catgaagatc
    18241 ttaaggcagg aacagggtat ataaaaattt tctttaatat gttaccataa aataggtaac
    18301 aaaacattta taagtaacta ctcattatta gcgctcatgg gaaagcattt tggatttttg
    18361 gtgctcatag cagaaaaaac aggccagttt ggcatgctaa agacatccac cttaagcctc
    18421 tcaaaagagc tgggtgtccc ccagcagacg ataagccgca agcttaaaga tatggaacgg
    18481 ataggcctga taaaaaggca ggttagccca aaaggaatta tcctaagcat caaagatgaa
    18541 ggcagaaagc tcttgcagaa aaattatgag aagctaaagg aaattttggg cagggaaaat
    18601 cccataacag gcactgtcga aaaaggtata ggtgaaggtt cctattacgt ctctcaaaaa
    18661 ggctatcagt ctcaattcaa ggaaaagcta ggttttattc cctttcaggg aacattgaac
    18721 gtcaaggtcg gcgaagaagc gctcgcccag ttgctttcaa agccgcagat aaccattgac
    18781 ggattttacg ggggcaatag gacgtttggc tccatcagct gctacaaaat taagattagc
    18841 agtatagatg ctgtcatcgt aaggccagaa agagcctcgc atccaagcga tattttggag
    18901 ataatcgctc caataaacct gagaaatgct cttaagctta aagacaaaag caaggtgaag
    18961 ctaaagtgaa agtaggcata gcagacacaa tgtttgcaag gcaggatatg ttcaagatag
    19021 taaaaaaagc cctggaagac tctatggaac caggcatacg gcttgaaaga tatactgttc
    19081 caggcgtaaa agacctgccc gttgcctgca aaaagctctt tgaagaatat ggttgcgata
    19141 tatgcatggc cctgggaatg ccaggtgaca agcctatcga taaagtatgc gcccatgaag
    19201 cctcaaatgg cttgatccat gttcaattga tgaccaacaa acacataata gaggtttttg
    19261 tccatcttga tgaaggaaaa gatgaaaaag agcttttgga gatagtgcat aaccgggttt
    19321 atgatcatac actaaacacg ttagccctac ttaagggaaa agaggctctt agtaagaaag
    19381 ccggaaaagg cataaggcag ggaaaagaga gtgtaggaga actaaggtga gaatatggca
    19441 aatgaaaaac taggatttgt tgtttcagac tttaatgcag acattaccca tctaatgagt
    19501 aagcttgcac aggagcatgc agagtttttg caggcaaagg tggcaaaagt ctacagagtt
    19561 ccaggagcat tcgacatgcc tcttgcagta agtaagcttg tcaatatgca ggacatcgat
    19621 ggagtagtct gccttggagc agtaatcgag ggagacaccc agcatgatga gattgtggca
//
LOCUS       DASRUK010000048        13504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1554101, whole genome shotgun sequence.
ACCESSION   DASRUK010000048 DASRUK010000000
VERSION     DASRUK010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 13504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13504
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554101"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VFF28_02235"
     CDS             <1..196
                     /locus_tag="VFF28_02235"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZX44481.1"
                     /translation="GKKLVSFAESYCKDQAKKGIYLYTHPLHKAAIKLYKKLGYQKVN
                     VFPNYYSNGDASILYGKLLK"
     gene            193..1329
                     /locus_tag="VFF28_02240"
     CDS             193..1329
                     /locus_tag="VFF28_02240"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HZX44482.1"
                     /translation="MKNLSLLGSTGSIGRQTLDIVRKFPDRFKIVGLGCNSNVELLRE
                     QIDKFNPEIVCVYDEAAADQLKTDVQVYKGMPGLLKLASLGNSDTVVNSLVGSIGVLP
                     TVKAIENLKNVCLANKETLVTAGQIVMDKVKMHKTILLPIDSEHSAIWQCVKGYKMAD
                     VKKMTLTCSGGAFRDKSKEELANVKAADALLHPTWNMGAKITIDCATLMNKGFEVIEA
                     NHLYGTDYSKIDVVIHPQSIVHSFVEFSDNSVLAQLGEPDMRIPIQFALMFGERVTNE
                     SLPLLNMAGKMLSFYAPDVDRFPCLPMAYNCGVMGGNAPAVMNAANEVCVKYFLQDKI
                     GFLDIPLIIREMIEKQAFVKRPNLDELMSVDQKVKDETERRIIG"
     gene            1326..2483
                     /gene="ispD"
                     /locus_tag="VFF28_02245"
     CDS             1326..2483
                     /gene="ispD"
                     /locus_tag="VFF28_02245"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HZX44483.1"
                     /translation="MNYAIIVAAGEGKRMKSRVNKILMMLDDKPVIYHSILPFNNSAL
                     IDRIILVARREDIVDINHLVKESKFSKVIIAEGGEERQDSVFNGIKAIKEAKADDIVL
                     VHNGANPFVDDKLIEEVIDSAKQDGACAAAIRAKDTIKEVDLEGYVVKTLERKKLWQM
                     QTPQAAQYKILVKALVKASNDEYIGTDDAELIERSGHKVKIIECSKDNFKVTTPSDLE
                     NAKLVLEADRIGFGMDSHKFSTDEKELVLGGVVIPNEEGLAANSDGDVVLHSLFNALS
                     SACGGKSIGFYADPMCKKGMKDSSAYLQVALEMVKEKGLKINNIGIMVEGAKPKLDEF
                     SDKIKERIAQICDIDKDKVGFCATSGEELSDFGKGKGLQVFSIVSLRREKV"
     gene            2480..3262
                     /gene="ispE"
                     /locus_tag="VFF28_02250"
     CDS             2480..3262
                     /gene="ispE"
                     /locus_tag="VFF28_02250"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HZX44484.1"
                     /translation="MKAYAKLNLCLDVFGRRQDGFHEINSIMQQIDLADELTFNDSDE
                     VIVDSPFTDDIMLKAARLVKERFKIRDGVKIMVEKKIPHQAGLGGGSSDCAVALAVLN
                     KKWELGLSLEQLALLGAELGADVPFFLYGGASFAAGKGERVVPLKMPKLNFLIVKPDY
                     SISTKWAYDELDKVKYNAKHTSARVRDGGSVIENMHNDFIHIQQEEVKEIITQMKDNG
                     ALCASITGKGPTVFGIFDSRADAISASKDFSEHPFVYAGRSI"
     gene            3259..4083
                     /gene="ispH"
                     /locus_tag="VFF28_02255"
     CDS             3259..4083
                     /gene="ispH"
                     /locus_tag="VFF28_02255"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HZX44485.1"
                     /translation="MNVRVAGESGFCFGVRKAIERVKDIKDKSNVFVAGKLIHNPQVI
                     GSLEREGVRFVDSIEGVGDGILVVSAHGLPDNVIEKAREQGLEIIDTTCPLVKNVHNI
                     TKDLERQGARIIIFGDKNHTEVKGIVGNLTNPIVISDISEIKDIPDGNYALVSQTTQE
                     IGNFNLIGNELKKSFPDSLVSDTICSATKLRQRSAEELAHMSELMIVIGGKDSANTKR
                     LALICSRITDTKQIERIEDVQREWFLGKTNIGVSAGASTPQEVIKDIVEYIKRITS"
     gene            complement(4064..5206)
                     /locus_tag="VFF28_02260"
     CDS             complement(4064..5206)
                     /locus_tag="VFF28_02260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZX44486.1"
                     /translation="MTLREQECFQNKLYDVAIIGAGPAGLMAANTFSSSVNFIILDPK
                     KEIGLPLRCGEGVREKEFERLFGKRSYPFIRNHVHNHEVRYNSLKRSFKADYIQLDRP
                     AFEKYLGKNFKGKLLLNTSCKDIIIKKDHALVITSKGTIKAKLVILAYGPNFRIQKKL
                     KLQKKTPTLCINYGGIYKNHGFPTDRFYAYFFDKYFGYLWVFPKDKATANIGFCTIVK
                     GINVKQVLEKALKDINPGLKQLSSYSGIFPCDGPIEKTYSNRLLVCGDAAGLVYAGTG
                     EGIYYALESGRLAGLTAKNALKNNLFSKDFLKTYETAWKKSFGKQMTAGIAFRDLQYL
                     AFRAKKMKELFKAPTNKELNMLLSGRIPLRARIPWYIYRIAKKLSS"
     gene            complement(5172..5627)
                     /locus_tag="VFF28_02265"
     CDS             complement(5172..5627)
                     /locus_tag="VFF28_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HZX44487.1"
                     /translation="MARRFSRRKGKAGSNKPIHPAIPSWVRYKEKEVELLVTKLAKEG
                     KSPSQIGILLRDIYGIPNSRLILKKSITEMLGEKKLLGEIPEDLLSLIKKRAAIGKHL
                     EMNKKDETAKKGVILVEAKIRNLLRYYKKVGKMPSEWKYDPKRAGMFSE"
     gene            5993..6307
                     /locus_tag="VFF28_02270"
     CDS             5993..6307
                     /locus_tag="VFF28_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44488.1"
                     /translation="MLDFLITHSELDYSLKEISRYSGASYSSVKILVKKLAEDKWVVA
                     TRKISKIILYKLNTENPEVKKFVDFYWEVIEQEIKGKEEPCNYCGGSASNFGLAASAR
                     NL"
     gene            complement(6377..6787)
                     /locus_tag="VFF28_02275"
     CDS             complement(6377..6787)
                     /locus_tag="VFF28_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44489.1"
                     /translation="MKIISLRKGYECDHSTRDYGYISGVKVFYDYGTFDMEFAVPYDT
                     ELFKLLKVNKDIDAEKKFNKMVISLYLYCDGFNEDEGKYVELALAVRKAIINKNLEVI
                     KIINAYHGSKDSFSKMKAETTLGKRLQKVLTACW"
     gene            complement(6841..7080)
                     /locus_tag="VFF28_02280"
     CDS             complement(6841..7080)
                     /locus_tag="VFF28_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44490.1"
                     /translation="MKTGTNIKLSKSELCILYRAIKIMQSGEALLGIDIDRKVVPVLA
                     KEILKYIDAKTAKKIDKEAALLVKAAEEASEDYND"
     gene            complement(7064..7642)
                     /locus_tag="VFF28_02285"
     CDS             complement(7064..7642)
                     /locus_tag="VFF28_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44491.1"
                     /translation="MKILSQRKGFDGDHSSVSYEFISNDISKDAKDFARESSRHFKIH
                     DKRLKIQIFGEHYLNQDIQDTLLEKLGIPLLIYEDYDSWNFIIMFEFDKKLMKELENF
                     DGVGEEDTIHVSKKGQKIELWITVHINYGCFDESPFEDLGDMFMKIRKKILEYDFESL
                     LILKRYCNGESKFNATSDLYKRLQSILNENRH"
     gene            complement(7629..8903)
                     /locus_tag="VFF28_02290"
     CDS             complement(7629..8903)
                     /locus_tag="VFF28_02290"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZX44492.1"
                     /translation="MNQNLYVRSKETILRKEFFGGIAFNKKKAITIELDEEGFYLLSI
                     LDKPITIKNIALKLSSTFGRIFSFKEIQSNLELLQQQGFIGNKPFSKSKGGPDNLPQL
                     SSGSTFLSAPETVHLSITGKCNLHCAFCYGKSDTPDLSTAQIFSLIDELSSMSVFQLA
                     IGGGEPFLRKDIFKIINYCRQKNIVVNLTTNGALLTDFTARNIKNKVGQVNLSYNEDP
                     TGQFKRALDILLKNKINTGINLLITKDILSRLSLVIEEMLSYKIAKIIILRPKPCPNK
                     NWYEKNRLTKSELLELKRILNKYSQGINVDCSLTCLMHDISPEELRENAVYGCVAGAR
                     FCTIRNNGDVYPCSFFSTEEFCAGNVVDSGFGIIWQKTQIFKKFREMNRYTKGGCASC
                     RIRDYCKGCRSVILEAEQDFYSQDKECPNEDS"
     gene            complement(8921..9838)
                     /locus_tag="VFF28_02295"
     CDS             complement(8921..9838)
                     /locus_tag="VFF28_02295"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZX44493.1"
                     /translation="MLSQKEIQKIASILAKKFPTVLSIIQFGSSVSGDFTKFSDTDIA
                     VIIKDKKSEKKVRDFCFSCHSAFELQIHLFSSDKFINDLKKGMPLALSILYTGRAIYG
                     KERIQKLKRKNYLPNNFTKRACMLNSFAALSLAVSDLTNGMLADSLNSAYHAARSSIW
                     AVLMDRAITPQNKEALRLIADKKIKLLYKRILGFRAHPPDYGYDIALSQDIYKKGNIN
                     EYTQLLEDATYIVKKNYRKIFNRNFIGLFEVLEILNKRYKSPSHYSIYLLVDWEKEKL
                     LYMVFLSVNSSRIFLNIDVNSGKVCKARG"
     gene            complement(9838..10233)
                     /locus_tag="VFF28_02300"
     CDS             complement(9838..10233)
                     /locus_tag="VFF28_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44494.1"
                     /translation="MTPQISKPIGDYGHPNLSSSFFKNNPMQVFSVPMLCYNMILNPE
                     IEAKSIKQIYHSLLPSRKEKIQKERKKMEEAIDSTGLLEILNAGIDPINENKPLWDCY
                     HYLKEEYPDNSYSPGPLLALSLLANHGEI"
     gene            complement(10328..10420)
                     /locus_tag="VFF28_02305"
     tRNA            complement(10328..10420)
                     /locus_tag="VFF28_02305"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10364..10366),aa:Gln,seq:ttg)
     gene            complement(10603..11340)
                     /gene="dph5"
                     /locus_tag="VFF28_02310"
     CDS             complement(10603..11340)
                     /gene="dph5"
                     /locus_tag="VFF28_02310"
                     /EC_number="2.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004164 - diphthine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0017183 - protein histidyl modification to
                     diphthamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine synthase"
                     /protein_id="HZX44495.1"
                     /translation="MALYFIGLGLGDEKDITLKGLEAIEKADKVYLENYTSRLSGATR
                     EGLEKFYKKKIILADREMVESKADETILKDAKHHNVAFLAIGDPFSATTHVSLKLRAD
                     ELKIKTIVIANSSIINSVGITGLDLYKFGRITTIPFQNENITSPYEVVQNNLKNNMHT
                     LVLLDLDPSNEKFMTVSQAISYLLRVGMSKNQLCIGCAGIGSDDPQIKAAKAIDLEKK
                     KFTKYPQCIIVVSKLHFMEEEALKKYY"
     gene            complement(11505..12548)
                     /gene="ftsZ"
                     /locus_tag="VFF28_02315"
     CDS             complement(11505..12548)
                     /gene="ftsZ"
                     /locus_tag="VFF28_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HZX44496.1"
                     /translation="MDLTSKENLGLEVGQANIKVFGVGGAGNNMVGWLYSKGIKGAEI
                     IGANTDKQHLDIINADRKILLGRGVTRGLGCGGFPERGKESAQESLQEVKEVLKGSDM
                     VFVCAGMGGGTGTGAAPVVAQVAKDSGAIVIGTVTMPFRIERARIDRAEFGLQQLRQV
                     SDTVIVIDNNRLVTIAGNLPIQQAFAVANELVSTMIKGIVEIIAIPSLVNLDYADVKA
                     IMTNGGVAAIGVGTSDTTNKVEEAVRGALANPLLDISYKGATGALIHITGGPDMTLDE
                     INKAGELVTESLDADANVIWGARVEETMKGKLTVMSIITGVKSPWILGKVDYSRPSAH
                     SQRITEELGIELV"
     gene            13015..13302
                     /locus_tag="VFF28_02320"
     CDS             13015..13302
                     /locus_tag="VFF28_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44497.1"
                     /translation="MGLEDKVSGMAGGLGLLAGAAFGFYSGFHAYSLLAYYGKAVQYI
                     GTLIGGVAGAIAGNKVFKYASSGLNKLFSYASGKKKDGNEKDAKPGAAKAG"
BASE COUNT         3840 a         2615 c         2826 g         4223 t
ORIGIN      
        1 aggcaaaaaa ctggtaagtt ttgctgagag ttactgcaaa gatcaagcaa aaaaggggat
       61 atacctatat actcatcctc tgcataaagc tgcaatcaaa ctctataaaa aattgggtta
      121 tcaaaaagtg aatgtgtttc caaattatta tagtaatgga gatgcatcaa tactttatgg
      181 aaaactgcta aaatgaaaaa cttatcatta cttggctcta ccggaagcat cggaagacaa
      241 acgctggata tagttcgaaa attccctgac agatttaaga tagtggggct tggatgcaat
      301 tctaatgttg agcttcttag agaacagata gataagttta atccggagat cgtttgtgtg
      361 tatgatgaag ctgctgcaga ccagctcaag acagatgttc aagtctataa aggaatgcct
      421 ggacttctga agcttgcttc cctgggtaat tcggacactg ttgtaaactc tcttgttgga
      481 agcatcggtg tgttacccac tgttaaggct atagagaatc ttaagaatgt ctgccttgca
      541 aacaaagaga cgctggttac agccgggcag atagtgatgg acaaggtgaa gatgcataag
      601 acaatacttt tacctattga ttctgagcat tctgctattt ggcagtgcgt caaaggatat
      661 aagatggctg atgtaaagaa gatgacattg acatgctctg gcggggcgtt tcgtgacaag
      721 agcaaagaag aacttgccaa tgtcaaagcg gctgatgctc ttctgcatcc aacatggaat
      781 atgggggcta agatcactat agattgtgca acccttatga acaaagggtt tgaggtaatc
      841 gaggccaatc acctgtatgg aacagattat tcaaagatag atgttgttat ccatccgcaa
      901 agcatagtgc atagctttgt agagttttca gataactctg tgcttgcaca acttggagag
      961 cctgacatga ggatacccat ccaatttgcg ctgatgtttg gtgagagagt tacaaatgag
     1021 tctttgccgc tattgaacat ggctggaaag atgctttcat tctatgcgcc tgatgtagat
     1081 agattcccat gtttacctat ggcgtataac tgtggtgtga tgggagggaa tgcgcctgct
     1141 gtgatgaatg cagcaaatga agtctgcgtc aaatattttt tacaagataa gataggattt
     1201 ttagacatac ccttgataat cagagagatg atagagaagc aggcttttgt taaaagacca
     1261 aaccttgatg aactgatgag tgttgatcag aaagtgaagg atgagacaga aagaaggata
     1321 attggatgaa ctatgcgata atcgttgcag caggagaagg aaaaaggatg aagagcaggg
     1381 taaacaaaat actgatgatg ctggatgaca agcctgtcat atatcattca atactgcctt
     1441 ttaataattc tgctttaatc gacaggatca tccttgtcgc aagaagagag gatatagtgg
     1501 atatcaatca cttggtaaaa gagagcaagt ttagcaaggt gataatcgct gaagggggag
     1561 aggagaggca ggacagtgta tttaatggaa tcaaggcaat aaaggaagcc aaggctgatg
     1621 atattgtttt agtgcataac ggagcaaatc catttgtgga cgataagcta atcgaggaag
     1681 tgatagatag tgccaaacag gatggggcct gcgctgctgc aatccgggcc aaagacacta
     1741 taaaagaggt tgatttggaa ggctatgttg tcaaaacgct tgagcgaaaa aagctatggc
     1801 agatgcagac tccgcaggct gcacagtaca agatcctggt gaaggcactt gtgaaagcat
     1861 ccaacgatga gtatattgga actgatgatg cagaactcat cgaaagaagc gggcataagg
     1921 taaagataat cgaatgctcg aaggacaatt ttaaggtaac aacaccttct gacctggaga
     1981 atgcaaagct cgtacttgag gcggatagga tagggtttgg gatggacagc cataagtttt
     2041 ctactgatga aaaagagctg gtacttggag gagtggtaat tccaaatgaa gaagggcttg
     2101 ccgctaactc tgatggagat gttgttttac acagcttgtt taacgcctta tcttctgcct
     2161 gcggtggaaa aagcattgga ttctatgctg atcctatgtg caaaaaagga atgaaagaca
     2221 gctctgctta tcttcaagtt gcactcgaga tggtaaaaga gaaaggacta aagataaata
     2281 acatagggat aatggtggaa ggtgcaaagc caaagcttga tgaattctct gacaagataa
     2341 aggagaggat agctcaaatt tgcgatattg ataaggataa agtagggttt tgtgcgacaa
     2401 gcggtgaaga attgagcgac tttggaaagg gaaaggggct gcaggttttt tcaatagtca
     2461 gcttgagaag ggaaaaggta tgaaagctta tgccaaacta aacctgtgcc ttgatgtttt
     2521 tggcagaaga caagacgggt ttcatgagat caattccata atgcagcaga tagaccttgc
     2581 tgatgaattg acctttaacg atagcgacga ggttatcgtt gacagccctt ttactgatga
     2641 tataatgtta aaggcggcca gattagttaa ggagaggttt aagattagag atggggtcaa
     2701 aataatggtt gagaagaaaa tccctcatca ggcaggcctt ggtggaggaa gcagtgattg
     2761 cgctgttgca cttgctgtgc ttaacaaaaa gtgggagctt gggctttctt tagaacaact
     2821 tgcattgctt ggagctgagc ttggcgctga tgttcctttt tttctctatg gaggggcctc
     2881 ttttgccgct ggcaaaggag agagggtagt tcctttgaag atgcctaagt tgaatttttt
     2941 gattgtaaag cctgattatt caatttcgac aaagtgggcg tatgatgagc tggacaaggt
     3001 taaatataat gcaaaacata ctagtgcaag agtgagggat ggaggaagtg ttatagagaa
     3061 tatgcataat gattttatcc atatacagca agaagaggtt aaggagatca ttactcagat
     3121 gaaagacaat ggtgcgctat gtgcctcgat aaccggcaaa gggccgactg tttttgggat
     3181 atttgacagc agggcagatg ctatcagtgc atccaaagat tttagcgagc atccttttgt
     3241 ttatgcgggg aggagcatat gaatgtcaga gtggcaggtg agagtggatt ttgctttggt
     3301 gtgaggaagg cgatagaaag ggtcaaggat ataaaggaca aaagcaatgt gtttgtggca
     3361 ggcaagctca tacataatcc tcaggtcata ggaagcttgg aaagagaagg agtcaggttt
     3421 gtggacagta tagaaggcgt tggcgacggc atacttgtcg tctctgcaca tgggcttcct
     3481 gataatgtga ttgagaaggc aagagagcag gggcttgaaa taatcgatac gacatgccct
     3541 ctagtaaaga atgtgcataa tataacaaag gatcttgaga ggcagggagc tagaatcata
     3601 atatttggag ataaaaacca tactgaggtc aagggaatag tcgggaacct tacaaaccct
     3661 atcgtcataa gtgatatttc agagatcaag gatatccctg acgggaatta tgcccttgta
     3721 tcccagacaa cgcaggagat agggaatttt aatcttatcg gcaatgaact gaaaaagagt
     3781 tttcctgata gtttggtgag cgataccatc tgttcagcga caaagcttcg gcaaaggagc
     3841 gctgaagagc ttgcccacat gtctgaactt atgatagtga ttggcggcaa agacagcgca
     3901 aacacgaaga ggcttgcttt gatatgcagc aggattactg acacaaagca gatagaaagg
     3961 atagaagatg tacagagaga atggtttctt ggcaagacga atattggagt aagcgccggt
     4021 gcatcaaccc ctcaggaagt tatcaaagat attgttgaat atatcaagag gataacttct
     4081 tagcgatcct atagatgtac caggggatcc ttgcccttaa gggaatcctg ccgctgagga
     4141 gcatgttaag ctccttattt gtcggggctt tgaaaagctc tttcatcttt tttgccctga
     4201 aagctaagta ttgaagatcc ctgaatgcga tgcctgctgt catctgcttt ccaaagcttt
     4261 ttttccatgc agtctcgtag gttttaagaa agtctttgct gaaaaggtta ttctttaaag
     4321 cattttttgc tgtgagccct gcaagccttc ctgattccaa cgcgtaatat atcccttccc
     4381 ctgtgccggc atagacaagg cctgctgcgt ctccacagac caatagccgg ttactataag
     4441 ttttttctat ggggccgtcg catgggaaga tgcctgagta ggatgagagc tgctttaagc
     4501 ccgggttgat atcttttaat gctttttcca gaacctgctt tacgtttata cctttaacta
     4561 tggtgcaaaa gccgatgttt gctgtggctt tgtcttttgg aaaaacccag agatagccaa
     4621 aatatttatc gaagaaatat gcgtagaacc tatctgttgg aaagccatgg tttttgtata
     4681 tgccgccata gtttatgcat aaggtggggg tcttcttttg gagctttagc tttttttgta
     4741 tcctgaagtt tgggccgtag gctagaatca ccagctttgc cttaatggtg cctttgcttg
     4801 tgatgacgag ggcatggtct ttttttatga ttatatcctt acaggaggtg tttaacagaa
     4861 gtttgccttt gaagtttttt ccaagatatt tttcgaatgc aggacggtcg agttggatgt
     4921 agtctgcttt gaaagagcgc tttagagaat tatacctgac ttcatggttg tggacatggt
     4981 ttcttatgaa tggatatgat ctttttccaa ataatcgctc aaactccttt tcccttactc
     5041 cctcgccgca tcttagagga aggcctatct ctttttttgg gtcaagaatt ataaagttaa
     5101 ctgaagaaga aaaagtattt gctgccataa ggccggctgg accagcccca attatggcaa
     5161 cgtcatacag cttattctga aaacattcct gctctcttag ggtcatactt ccactcagag
     5221 ggcatcttgc cgaccttttt gtagtatctt aaaaggttcc tgatctttgc ctcaacaagg
     5281 ataacgcctt tttttgctgt ctcatccttc ttgttcatct caaggtgctt tccaattgct
     5341 gcccgctttt tgatgagcga aagcagatct tctgggatct ctccaagaag ctttttttcc
     5401 ccaagcatct ctgtaatgga tttctttagg ataagccttg aatttggaat gccatagata
     5461 tcccttaaca atatccctat ctgagaaggg ctttttcctt cttttgcaag ctttgtaacc
     5521 aacagctcaa cttctttttc tttatatctt acccatgagg gtatcgctgg atgtatgggc
     5581 ttatttgagc cagcttttcc ttttcttctt gaaaatcttc gtgccatttt ttcaccttcg
     5641 ggacaaataa gaaatatatt tgtcgccagt ataagcaagc ctttagaccg gcttatctct
     5701 tgatatggat tagaactata ggtttattta taaggatgtc gatttataac atgagcaata
     5761 ctggaataat cgattcttta cctgtttcct taattatctc ctttatctta tcataacgat
     5821 ccatatttag atagtatttc catccatgct cttttttctt tctgcaaaca atcctttgct
     5881 taacaagagc atcaatattt atatattagt gtgtatacca atattgttaa taatggtatt
     5941 tatgataact tcaattttta taaaaaaaca aggagaatcg ccgaaaaaca aagtgttgga
     6001 ttttttaatt acgcactctg aactagatta ttcccttaaa gaaatatcca ggtattctgg
     6061 tgcaagttac tccagcgtaa aaattcttgt aaagaaactt gctgaggata aatgggtcgt
     6121 ggctaccaga aagatatcaa agattatctt atataagcta aatactgaaa atccagaggt
     6181 taagaaattt gttgatttct attgggaagt gattgaacag gagattaagg gaaaggaaga
     6241 accttgtaat tattgcggag gctctgcttc aaactttggc cttgctgcct ctgccaggaa
     6301 tctttaaaac cttttgctgc tcatttactg aggtagttga gaatccgaat tctcttctga
     6361 caatgcgacc tctagcttac cagcaggctg ttagaacttt ctgcagcctt ttgcctaatg
     6421 tagtctctgc cttcattttc gagaagctat ccttactgcc atgatatgca ttgataattt
     6481 taattacttc cagattcttg tttattatgg cttttcttac tgccagagca agctcgacat
     6541 atttgccctc atcttcatta aagccatcgc aatataagta taggctaata accatcttgt
     6601 tgaacttttt ttctgcatca atatccttat tgaccttaag aagcttaaac aactctgtgt
     6661 cgtagggaac tgcaaactcc atgtcaaaag taccgtaatc atagaagacc ttcaccccag
     6721 atatatatcc atagtctctt gtactgtggt cgcactcata tccctttctt aatgagatta
     6781 tcttcatttt ttctcaccct tataattttt tggccctctg tcaaagtgat cccctcacca
     6841 ttaatcatta taatcctcgc tggcttcttc tgctgctttt accaacaagg ctgcctcttt
     6901 gtctattttt ttggctgttt ttgcatcaat atatttaagg atctccttag ccaagactgg
     6961 aacaactttc ctgtcgatgt caatgcctaa cagcgcttcc ccagactgca ttatcttaat
     7021 tgccctatac aagatgcata attcgctttt gctaagcttt atattagtgc cggttttcat
     7081 tcaaaataga ctgcaatctt ttataaagat cagaggtagc attaaatttg ctttccccat
     7141 tgcaatacct ttttagtatc agaagtgatt caaaatcgta ctcaaggatt tttttcctaa
     7201 ttttcataaa catatccccc aagtcctcaa agggggattc gtcaaaacag ccataattta
     7261 tgtgtacagt tatccacagc tctattttct ggcctttttt ggatacatga attgtatcct
     7321 cttcacccac accatcaaaa ttttccaact ctttcattaa ttttttatca aattcaaaca
     7381 taataatgaa attccaagaa tcatagtctt catatatcag caagggtatg cccaactttt
     7441 ccagcagtgt gtcctgaata tcctgattca agtaatgttc accgaatatc tgtattttta
     7501 gccttttgtc atgaatctta aaatggcggc tgctttctct ggcaaagtct tttgcatcct
     7561 ttgatatatc attgctgata aactcgtagc tgacagaact atgatcgcca tcaaaccctt
     7621 ttctttggct aagaatcttc atttgggcac tccttatctt gactataaaa atcctgctct
     7681 gcctctaaaa taacgctcct gcaacctttg caataatccc ttatcctaca gctagcgcat
     7741 cctcccttag tatacctgtt catctccctg aactttttaa atatttgtgt cttctgccat
     7801 ataattccaa aaccagaatc aacaacattt cctgcgcaga attcctcagt gctgaaaaag
     7861 gaacaaggat aaacatcgcc gttgtttctt attgtacaaa acctggctcc tgcaacacac
     7921 ccataaacag cattttctct taattcctct ggagaaatat cgtgcattaa acaagtcagg
     7981 gagcagtcaa cattaattcc ttggctgtat ttatttaaaa ttctctttaa ctccagcagc
     8041 tcactttttg tcagcctatt cttttcatac caatttttgt tagggcaggg ctttggcctg
     8101 aggatgatta ttttggcaat tttatagctt agcatttctt caattactaa agaaagcctt
     8161 gaaaggatat cttttgtaat taacaggttt atccctgtat taatcttatt tttaagcagt
     8221 atatctaacg ctcgtttgaa ttgaccagta ggatcttcat tatagctcaa gttcacctgc
     8281 cctaccttgt tttttatatt tctagctgtg aagtcagtga gaagagcgcc atttgtggta
     8341 agattaacaa caatgttctt ttgccggcaa tagttgatta ttttaaagat gtcttttctt
     8401 aagaaaggct ctccgccgcc aatagccagc tggaacacgc tcattgaaga taattcatct
     8461 attaggctga aaatttgagc tgttgaaaga tcaggtgtgt ctgattttcc atagcagaaa
     8521 gcacaatgta agttgcattt gccagtgatg gataaatgga cagtttcagg ggctgatagg
     8581 aaagtggaac ccgaagagag ttgtggaaga ttgtcgggtc ctcctttaga ctttgaaaaa
     8641 ggtttattgc caataaaccc ttgctgctgc agaagctcaa gatttgattg aatctcctta
     8701 aaagaaaaga tccttccaaa tgtgctggat agttttagcg ctatattctt tattgttatt
     8761 ggcttgtcga ggattgaaag cagataaaat ccctcttcat ccaactctat ggtgatggcc
     8821 ttctttttgt taaaggcaat acccccaaaa aattctttcc gaaggattgt ttctttgctc
     8881 ctgacataaa ggttctgatt cataagccgc cccagtgcat ctatcccctg gctttgcaaa
     8941 ccttgccgct gtttacatcg atattaagaa atatccttga gctattaacg gaaaggaaaa
     9001 ccatatatag cagcttttcc ttttcccagt caaccaaaag ataaatggaa taatgagaag
     9061 gcgatttgta tctcttatta agaatttcaa ggacctcaaa gagtccgata aagttcctat
     9121 tgaatatttt gcgatagttc tttttaacaa tatatgttgc atcctccaat agctgtgtat
     9181 actcattgat attgcctttt ttgtatatgt cctggctaag tgcaatgtca tatccgtaat
     9241 ctggaggatg agccctgaat cccagtatcc ttttgtagag cagcttgatt ttcttatctg
     9301 caatcaggcg caatgcttcc ttattttgag gggttatggc cctgtccatg agcacagccc
     9361 aaattgagga tctggctgca tgataagctg aattcagcga atctgcaagc atgccattcg
     9421 tcaggtcaga aacagcaaga ctcaatgctg caaaagagtt gagcatgcat gctctttttg
     9481 tgaaattgtt tggcaggtaa ttctttcttt tcagcttctg aatacgctct ttaccatata
     9541 ttgccctgcc tgtgtataaa atgctcagag caagaggcat gcctttcttt aaatcgttga
     9601 taaacttatc acttgagaat aggtggattt gcaattcaaa ggctgaatgg cagctgaagc
     9661 aaaaatccct gacctttttt tcgctttttt tgtccttgat tattactgca atatcagtgt
     9721 cgctgaattt tgtaaagtct ccactgacag aggagccaaa ttggataatt gagaggactg
     9781 ttggaaattt ctttgctagg atacttgcaa ttttctggat ttctttttgg cttagcatta
     9841 aatttcacca tgatttgcta agagggataa agcaagtaaa ggccctgggc tgtaactgtt
     9901 atcaggatac tcttccttta gatagtgata acagtcccat aaaggtttat tctcattaat
     9961 tgggtctatt cccgcattta aaatttccaa cagcccagtg gaatctatcg cttcctccat
    10021 ctttttcctt tctttttgta ttttttcctt cctagatgga agcagagaat gataaatttg
    10081 ctttatagat tttgcctcaa tttcaggatt aagaatcata ttatagcaca acatgggtac
    10141 tgagaaaacc tgcatgggat tatttttaaa aaaagagctt gataaattgg ggtgaccata
    10201 atcaccgatt ggtttactta tttggggtgt cataataaaa tagccccggg cagattgccc
    10261 acctcagaac ctaggttccg agacctccgg ggtaatgcgg tcatttacaa ttaatggtaa
    10321 taaaatatag ccccgggcag attcgaactg ccgtcttcgg ctccaaaagc cgaaatgatt
    10381 gaccatcgtg catagtttta ctattctaca ctacggggct gtctctcttt ggagaaaggt
    10441 attttgttta taaaagtttc ttcaataagt tatttttaat aaagctaaca ataatggact
    10501 caaacacggt catgctacgc atgccctact cgtaaaggta aaaaccccac tccgtagggt
    10561 ttttccctct acactcgtgt tttcgtccat tgttagcaag aactaatagt acttcttcag
    10621 cgcctcttct tccatgaagt gaagcttgct tacgacaatg atgcactgag ggtatttggt
    10681 gaacttcttc ttttccaggt caatggcttt tgctgccttt atctgagggt catcactgcc
    10741 tatgcctgca cagcctatac ataattggtt ttttgacatg cctaccctta ggagatatga
    10801 gatggcctgg cttacggtca tgaacttttc atttgaagga tcaaggtcaa gaaggaccaa
    10861 ggtatgcatg ttgtttttaa ggttgttttg tacgacctcg taaggtgaag ttatgttctc
    10921 gttttggaaa gggattgttg taatccttcc aaacttgtac aggtcaaggc cggttatgcc
    10981 tactgagttg attattgaag agtttgctat gactattgtt tttatcttaa gctcatctgc
    11041 cctaagctta aggcttacat gggttgtcgc tgagaaagga tcgccaatcg ccaggaaggc
    11101 aacattatgg tgttttgcat ccttaaggat ggtctcatct gccttgcttt cgaccatctc
    11161 cctgtctgct agtataatct ttttcttgta gaatttttct aggccttccc tggtcgcacc
    11221 cgagagcctt gaagtatagt tttcaaggta aaccttgtct gctttttcta tggcttccaa
    11281 acctttgaga gtgatgtctt tttcatcacc aaggccaagg ccgatgaagt atagagccat
    11341 acctgcttag aaagtagact gctatttaaa cattatcaaa acaacaaatg cctctgtatt
    11401 tttaaaaaaa agaaaaaaaa ttgccttcac aaaacgttgg gggtggtttt taaatgaagg
    11461 caaaagaact ttctcaccac aaagactaaa ggggtgaata tgaactaaac tagttctatg
    11521 cctagctctt ctgtgatgcg ctgtgaatgc gctgacggcc ttgaataatc tacctttccc
    11581 agtatccaag gtgatttcac acctgtgatg atcgacatca ctgtaagctt gcctttcatg
    11641 gtctcttcaa cccttgctcc ccagatgacg tttgcatctg cgtcaaggga ttctgtgaca
    11701 agctctcctg ccttgtttat ctcatcgagt gtcatatctg ggccgcctgt tatgtggata
    11761 agtgcacctg tagcaccctt atagctgatg tcaagcaagg gatttgcaag agctcctctg
    11821 accgcctctt caaccttatt ggtggtgtct gaagtaccaa cgccaatcgc tgcaactccg
    11881 ccgtttgtca ttattgcttt tacatcggca taatccaggt tgaccaagct tgggatcgca
    11941 attatctcaa ctattccttt gatcattgtt gatacaagct cgtttgccac tgcaaatgcc
    12001 tgctgtatcg gcaggtttcc tgcaatcgta accaacctgt tgttgtctat gacaataact
    12061 gtgtctgata cctgccttag ctgctgaagg ccaaattctg ccctgtcgat ccttgccctt
    12121 tcaatcctga atggcatagt gactgtgccg ataactatcg ctcctgagtc ttttgcaacc
    12181 tgtgccacta ctggtgctgc acctgttcct gtgcctccgc ccattcctgc acaaacaaat
    12241 accatgtcag agcctttcaa tacctctttt acttcctgca atgattcctg tgcggattct
    12301 tttccccttt caggaaaacc gccacagcca agtcctcttg tcactcctct tcccagcaat
    12361 atcttgcgat cagcatttat tatgtccaaa tgctgtttat cagtattagc tccaataatc
    12421 tctgcgccct taatgccttt agagtataac cagccaacca tgttgttgcc tgctccaccc
    12481 acgccaaata cctttatgtt tgcctggcca acttccaacc ccaggttctc tttacttgtc
    12541 aagtccattt tcacagcccc cttttgtggt tagatactcc aaactgtatt tttgagtacc
    12601 gaaatattta tatctaggtt gattctaaat acttcttaga aaaagttaat cattcacgcc
    12661 aatgaattgt taactaagag agattaaatt aacacgaaat ttgctttcgc caaaaagcac
    12721 atttgaaaac acacacattc gaatttgagt tttcgccaaa aaactcaaaa ttctaaaatt
    12781 tttaaattta tttgattgaa gctgatattt aaatatttct ctaaactgct tagtggtaaa
    12841 agagttagct aggggtttat tgacggtaaa actataaatc taatgatcga ttggtcaaaa
    12901 aataagtgag ttaaaggtaa agaggtaagc acctttagcc aacttttctt ataagagccc
    12961 ttatgttttt attgttacta tacctttaaa aataactgcc gatttttagc aaaaatgggg
    13021 cttgaagaca aagtcagcgg tatggcaggc ggacttggac tccttgcagg ggcagcgttc
    13081 gggttctatt ctggttttca tgcttatagt ctgcttgctt attatgggaa agctgtgcaa
    13141 tatatcggca ccttgatagg aggggttgcg ggggctattg caggcaataa ggtctttaaa
    13201 tatgcaagtt caggcttgaa caagcttttt tcatatgctt caggtaaaaa gaaggatgga
    13261 aatgaaaaag atgcgaaacc aggtgccgca aaggctggtt agtctgttta taccgataaa
    13321 taaatcacta cttctaagta atatttaaat aggcatcggg attagagtat gactactaag
    13381 atgactgata aaaattcata aagatatctt cccaatccca tatttttgaa gttatccctt
    13441 caatcataca aggagttttg tgctccttgt aaggttcaat gaaattattg tatgcttgaa
    13501 agat
//
LOCUS       DASRUK010000049         4136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1881157, whole genome shotgun sequence.
ACCESSION   DASRUK010000049 DASRUK010000000
VERSION     DASRUK010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1881157"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..746
                     /locus_tag="VFF28_02325"
     CDS             108..746
                     /locus_tag="VFF28_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44498.1"
                     /translation="MQEYAWRRRLFIISCLHFGFGGFCSILSNDPICLLLQDSFQHIV
                     QKSFKEKSGCIFLGLAITLFSVSFKVNYHKAWFTLFGFIFSDWSVRSLLIVLVAISAL
                     VSMIKLKKREVKVIAYTSIFVIFFFGPLMFYYIPFVNKGVWKCDYAFDKNECLAALAM
                     ERGDAAICENIKDNQYNKDICVAGVTHDLSICSNISYDYERNKCISATAKTV"
     gene            complement(754..891)
                     /locus_tag="VFF28_02330"
     tRNA            complement(join(754..799,854..891))
                     /locus_tag="VFF28_02330"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(855..857),aa:Leu,seq:caa)
     gene            complement(931..1299)
                     /locus_tag="VFF28_02335"
     CDS             complement(931..1299)
                     /locus_tag="VFF28_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44499.1"
                     /translation="MKKEETPLFVGITGKNELRKSMLESSKGILELMKEQEKSKETRL
                     EKARIVSTLKSEIREVTKLINALRAGIPRVNGSGGCKAEQRKVEEKPRPAKIENADRT
                     EIEKLEDELAEIESRLNSMA"
     gene            complement(1302..1523)
                     /locus_tag="VFF28_02340"
     CDS             complement(1302..1523)
                     /locus_tag="VFF28_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44500.1"
                     /translation="MAEAQVFVKIDEYKDVLRTIGLIKSRINDAKNTLQKVKELKSQE
                     DSELESWDGKLKEIESKIDRIDQTLFEPM"
     gene            complement(1527..2141)
                     /locus_tag="VFF28_02345"
     CDS             complement(1527..2141)
                     /locus_tag="VFF28_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44501.1"
                     /translation="MGLLKFKKRKESFPSFADLDIPPEPPKMLRQTFEEQPTIPELPK
                     DDFELPELPPLDDNMLSDLPPPPEIKKEKKGFFSFLKTKKAPADLELPELPALEKIPS
                     IKMEEPEEAPEPAEVEEQETQTPAPAQQANVTLKRFIKLEDFRNIQDEIANAKAILRG
                     SDELFSKLEELGALEDKSFSDLSGNLRDVHNKIMFADKSLFKEV"
     gene            2285..3004
                     /locus_tag="VFF28_02350"
     CDS             2285..3004
                     /locus_tag="VFF28_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44502.1"
                     /translation="MPSVALLKIKSVARKIFRVSKVILTASIPMNFLLLAVIIFISVA
                     SFEYFEPEELPVGDVVLEPVDCEPVYLESKPDCNLCPIKTKIEQTTKTVYRCPHGETV
                     EDLDDCKFFLPNVSALPSGVVDGITLAIGSIQFEDDGAQGGFVTAVEYTIINQGKAPI
                     VPKLEVKTYDEWTLKAKKSEPNKVIIPEIVINPGDFVKRTDKVRIYFEGDKQTVRLLL
                     VDSLPAIEKDIIAVAKDIKLG"
     gene            3131..>4136
                     /locus_tag="VFF28_02355"
     CDS             3131..>4136
                     /locus_tag="VFF28_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44503.1"
                     /translation="MYDPEGSDNNKEYIELYDEASLDLSAFTAGDSSSNDSLALLNLA
                     NSTFCLIAEEGFNFTGLNVTVYSVGSAIGNGLNKEDSIYIYANGTLLANLSYHDPCEE
                     GYSIEYFNSTYACSLYQGGTPGKRNSREFDYPKGIKINEFLPNPQGDDKEPAPDGEFV
                     ELCSITQASLRGYYLEDLSGRAVYIADTSSSPSTNVNDSCIAVFTNSYFLNNEGYEEI
                     SLYDPFGNLIDTVSYASSEESLSWSLFGDTWRYAFPTPAKGNERKEIGPQSAMKIISI
                     DDLDSNGEAQFGDLIRVNFFVYKANTTKSSVKLYIETDKERISKVAKVDLPNKYTNYS
                     L"
BASE COUNT         1159 a          842 c          828 g         1307 t
ORIGIN      
        1 ggcaagacta tcatatttta acaggattca gggataattt gtgcgcaaag cccatgggac
       61 tttaccgtta tacaaatggc agatagattt ttataaacat gtgtctggtg caagaatatg
      121 cttggcgaag aaggttattt attatttctt gtcttcattt tggcttcggt ggtttctgtt
      181 ctattcttag caatgatccc atttgtctgc ttttacaaga tagtttccaa catattgtac
      241 aaaaatcttt caaagagaaa agcggatgta ttttccttgg tcttgcgata accttattta
      301 gcgtctcctt taaagtaaat tatcataaag cgtggtttac actatttggc tttattttta
      361 gtgactggtc tgttaggtcg ttgctcatcg ttctcgttgc catctcagca ctcgtttcaa
      421 tgattaagtt aaaaaaaaga gaagtaaaag ttattgctta tacttcaatt tttgttattt
      481 tcttctttgg gccattaatg ttttattata ttccttttgt aaataagggt gtgtggaaat
      541 gcgattatgc gtttgataaa aatgaatgtc tagcggcgtt agctatggag agaggtgatg
      601 ctgctatctg tgagaatatt aaggataacc aatataacaa ggatatttgc gttgccggcg
      661 taacccatga tctgtcaata tgttccaata tatcctatga ttatgaaaga aataaatgca
      721 ttagtgcaac ggcaaaaact gtctaaatta aaatgcgagg aggaggattc gaacccccgt
      781 aggcactaag ccaatagatg acttacaaat gattctttca ccgcactctt tcgagcactc
      841 aacactcaaa tgccttgagt ctatcccgtt tggccgctcc ggcatcctcg caataattta
      901 aaagaaaaga gaaattctat aaaaatgtta ttaagccatg ctatttagcc tgctttctat
      961 ctcagcaagc tcatcttcaa gcttctctat ctcggtccta tcggcgttct ctatctttgc
     1021 tggcctgggc ttttcttcca ctttcctctg ctcggccttg cagccgccgc ttccattcac
     1081 tcgaggtatt ccagccctaa gggcgtttat cagcttggtg acttccctta tctctgactt
     1141 taatgtggat acaatccgcg ctttctcaag tcttgtttct ttggattttt cctgttcctt
     1201 cataagttcc aggattccct tggaggattc aagcatgctt tttctaagct cattcttccc
     1261 ggtgatcccg acaaataacg gggtttcttc ttttttcatt ttcacatcgg ttcaaatagt
     1321 gtttggtcta tcctgtctat ttttgactct atctccttta gttttccatc ccacgattca
     1381 agctcagaat cttcctggct tttaagctcc tttacttttt ggagtgtatt cttggcgtca
     1441 tttatcctgc tctttatcag gccaatggtt cttaatacat ccttatattc atcgatttta
     1501 acaaaaacct gggcttctgc cattttttac acctctttga ataatgattt gtctgcaaac
     1561 attatcttgt tgtgaacatc cctaaggttg ccgcttaaat ctgaaaagct cttatcttca
     1621 agagccccca gctcttcaag ctttgagaat agctcgtctg accctctaag tattgccttg
     1681 gcatttgcta tctcatcctg tatgttcctg aaatcctcaa gcttgataaa cctctttaag
     1741 gtcacgttag cctgctgggc aggcgcagga gtctgcgtct cttgctcttc aacctctgct
     1801 ggttcaggcg cttcctcagg ctcctccatc tttatagaag gtatcttttc aagcgctgga
     1861 agctccggca gctcaaggtc agcaggtgct ttttttgtct ttaggaatga gaagaatcct
     1921 tttttctcct ttttaatctc tggaggtggg ggaaggtctg acagcatgtt atcgtctaaa
     1981 ggagggagct caggcagctc aaaatcatcc tttggaagtt ctggtattgt aggctgttct
     2041 tcaaaagtct gccggagcat ctttggaggc tcaggcggta tgtctaggtc tgcaaaactt
     2101 ggaaaacttt ctttcctctt tttgaatttt agtaatccca ttttccaccc cttgattgaa
     2161 aatttaaaac agcattatta aaactttgtg tagcattagc aaatagtaac agagcaagcc
     2221 tttgctctat gaaagaaccg ccaaaataaa aaaatattta taggaccttt tatcataaat
     2281 tcctatgcca agcgttgccc ttctaaagat aaaaagtgtt gcaagaaaga tttttagggt
     2341 ttccaaggtg atattgactg cctcaatacc gatgaacttt ctgctccttg cagtgattat
     2401 ctttatttca gtagcttcat ttgaatattt tgagcctgaa gaattaccgg ttggagatgt
     2461 ggttttggag cccgttgatt gtgagccagt ataccttgaa agcaagcctg actgcaatct
     2521 ttgccccatc aagacaaaga tagaacagac aacaaagaca gtctatagat gccctcatgg
     2581 agagaccgtt gaagatttgg atgattgtaa gttttttttg cctaatgtca gtgccctgcc
     2641 tagtggagtg gtcgatggta ttactttggc tataggcagc atccagtttg aagatgacgg
     2701 cgcgcaagga ggctttgtta cagctgtgga atacacaata atcaatcaag gcaaggctcc
     2761 gatagttcca aaacttgaag taaaaaccta tgatgagtgg acattgaaag caaaaaaatc
     2821 agagccaaac aaggttataa tccctgagat tgtcataaat ccaggtgact ttgtaaaaag
     2881 gaccgacaag gttaggattt actttgaggg ggataagcag acagtgcgcc tgctgttggt
     2941 agactcgctc ccggctattg aaaaagacat tatcgcagtc gcaaaagata taaagctcgg
     3001 ctaagacaaa catttaaata attctcttac ccgccaagat tgggcggtta aaattaatcg
     3061 attatttcat tgtttggcag ctttattact gtttatttca ccaacttact ctatctatat
     3121 cagcgaagtg atgtacgatc ccgaaggctc agataacaat aaagaatata tcgagctcta
     3181 tgatgaagct tcacttgacc tatcagcctt cacagcaggt gattcatcat caaatgattc
     3241 tcttgctctt ttaaatttgg ccaactccac attttgcctg atagcagaag aagggtttaa
     3301 tttcacagga ttaaatgtaa ctgtctattc agttggttct gccataggta atgggcttaa
     3361 taaggaagat tctatctata tttatgcaaa tggtaccttg ctagcaaacc tctcatacca
     3421 tgacccatgt gaagaaggtt attccataga atattttaac agcacctatg catgcagcct
     3481 ctatcaggga gggacccctg gcaaaagaaa cagcagggag tttgattatc caaaaggcat
     3541 caagataaat gagttcttgc ccaatcccca aggagatgac aaagagcctg ccccagatgg
     3601 agagtttgtc gagctctgct caataacgca agcaagcctt aggggctatt atcttgaaga
     3661 tctaagcggg agagcagttt atatcgcaga cacctcaagt tcaccaagca caaatgtaaa
     3721 tgacagctgt attgcagtgt ttacaaacag ttatttcctg aacaatgaag gttatgagga
     3781 gataagtctt tatgatcctt ttggaaacct gatagacaca gtgtcttatg catcaagtga
     3841 ggagtctctt tcctggtctt tatttggaga tacctggcgc tatgcttttc caacccctgc
     3901 aaaaggaaac gagagaaaag agattggacc tcaatcggcc atgaagataa tatctataga
     3961 tgacctagac agcaatgggg aagcgcaatt cggtgacctc ataagggtaa acttctttgt
     4021 ctataaggca aacaccacaa aaagcagcgt aaagttatac attgagacgg ataaagaaag
     4081 gatctcaaaa gtggcaaagg tcgacctgcc aaataaatat acaaattata gcctca
//
LOCUS       DASRUK010000050        12021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_214029, whole genome shotgun sequence.
ACCESSION   DASRUK010000050 DASRUK010000000
VERSION     DASRUK010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 12021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12021
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214029"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VFF28_02360"
     CDS             <1..351
                     /locus_tag="VFF28_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44504.1"
                     /translation="VKSKQLEVKLERVLERMEEQGQNTTDIQPVIDRFNAKIESARIS
                     YETALGKFKQAINEADAEKAKDLAGEGNGFMKEAQKSLQEAQKVLKEVIQKIKSDGGE
                     IDAPEDAAQELAEA"
     gene            428..631
                     /locus_tag="VFF28_02365"
     CDS             428..631
                     /locus_tag="VFF28_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44505.1"
                     /translation="MLRKLAFMALVFIVLAGCQQQAAAPAGTVAPTEQKASSDLGVEE
                     INSLNQEIAPPENIETELDQVNW"
     gene            665..1723
                     /gene="rtcA"
                     /locus_tag="VFF28_02370"
     CDS             665..1723
                     /gene="rtcA"
                     /locus_tag="VFF28_02370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HZX44506.1"
                     /translation="MISLDGAFLEGGGQIIRTALGLSALTGRPFEVKDIRKGRREPGL
                     KNQHLFGIKAFEELCGAKAEGAALGSTAITFAPGKIKPQTLSIDIGTAGSISLLLQSL
                     LIASTFSKGKIRLKLTGGTDVIWSPQFDYLNNVILPQLRRYAEIETSLQKRGYYPKGA
                     GKVEIKIKSEYGMENISEAPKINLDQQYNLIQIKGISHASLDLQKAEVAERQARQAKL
                     LLTKLNCPIQIRTEYSDTLSTGSGITLWSVFSKDPGDIDIKNPIILGADSLGERGKRA
                     EEVGKIAAEKLIKGIESKAPVDSHLADNLIPYIALAKGKIKVSEISNHTLTNIYTCEQ
                     FLGKVFTVDSENRIIEAK"
     gene            1799..1969
                     /locus_tag="VFF28_02375"
     CDS             1799..1969
                     /locus_tag="VFF28_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HZX44507.1"
                     /translation="MEKLKCSSCKSIITNLEGSTRFACPSCGKTTIIRCNHCRDVGTK
                     YTCAECNFEGPN"
     gene            1972..2250
                     /locus_tag="VFF28_02380"
     CDS             1972..2250
                     /locus_tag="VFF28_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006448 - regulation of translational
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 1-beta"
                     /protein_id="HZX44508.1"
                     /translation="MANAVVTVRIMPESPDIDLNSIDSEAKKVIESIAGKGETKSSQI
                     PVAFGLKSLNIIFVMDEKKGSPDPVAEGIAKIPGVNSAEISDVRRAIG"
     gene            complement(2240..3283)
                     /locus_tag="VFF28_02385"
     CDS             complement(2240..3283)
                     /locus_tag="VFF28_02385"
                     /inference="COORDINATES: protein motif:HMM:NF014103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(26)-N(2))-dimethyltransferase"
                     /protein_id="HZX44509.1"
                     /translation="MYKLMQEGMAKLKVPVVKIVSKQMPVFYNPVMKFNRDVTVLLLN
                     TIDKWNMNIADPLAGSGVRSVRLLLELKTGKISSLGVNDHSSNACSKIKESLKLNKVK
                     SKNATVSNCDANLFLLNSNGFDFIDVDPFGTPNPFLDCAVKRISREGILAVTATDTSA
                     LSGTYPKSCLRKYWAKPVRSELMHEIGLRILIRKVQLIGVQYDKALFPIFSFSKDHYM
                     RVFFRCDKSKEETDNVLRQHGLFLDAGPMWLGKLWDEKLADKMAKENTVNENAKILRI
                     IAEESLIDTVGFYDLHKIAKKEKIEIPRKLELLEKLRKKGYLASETHFSGTGIRSDIR
                     IGELLDLLIITIQ"
     gene            3469..5202
                     /locus_tag="VFF28_02390"
     CDS             3469..5202
                     /locus_tag="VFF28_02390"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HZX44510.1"
                     /translation="MGEDWDFGQPSVDASLDELIAELRTHKKCIKSAAIGSKQYKTLD
                     LVIEAYKAHPLQVPDKLVYHLLELDIIPLIDFEGRYYFAVMYEGKSAGTTRSSSNIIV
                     NLEDNLFARDSRFNYDNARLILLKREEYQAVYQAVQGGPVEPAPRTEVKKPLVQLDPI
                     HKLIYKIVSTAVAKRATDIHFERVGTDNTRIRYRIDGHLVVYDDSIEPDLMRRVTSAI
                     KTMAEVKSYDPLDPQDGVITFKPQIVELFPNLKDYSLRLSTVPVKCDAATKEVDCLAL
                     RLHEAEIGRHTLGELGFPPEVVRQIYSVISASQGLMLVTGPTGSGKTTTLYSILDHLN
                     KPERNILTIEDPIEAFIPGLNQSQVNKERGWTFEKVMRHYLRQDPNVILVGETRDEET
                     AKLLFSAAETGHFVFSTVHTNSAYDTLTRVFELGIAPTYVVSSLSAVLSQRLARLLCT
                     DCKKDYDATAQIEEIFRTQLSHPLVLYRPGDIDCKRCHGTGYYGRIAVPELWVLDEEA
                     RDMVAAGEKYTSRYRQAAQKKGAKTLMQNGLELVLAGKTSLPEIARLSQSGLYYEKAY
                     LLPTVLERQKG"
     gene            complement(5226..5738)
                     /locus_tag="VFF28_02395"
     CDS             complement(5226..5738)
                     /locus_tag="VFF28_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44511.1"
                     /translation="MRYDQRRASYLQYAVHTIPRAQKSECLGLEKSLIEKNNFVLMGD
                     HYGHPDPRYPFVVTLSRSNKNKGYFYSGYLVGKGLEDKVDAMKEAVNNAVGNTTERHV
                     NDIQYVGGCLLIRYALSFTEKEMQEKEKINDISTEIHGKVNQALGKFAKVMMKNIDPE
                     KVYHAFKRGG"
     gene            complement(5748..6095)
                     /locus_tag="VFF28_02400"
     CDS             complement(5748..6095)
                     /locus_tag="VFF28_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44512.1"
                     /translation="MKWLALFLLGLFVVSPILGRYYIQQPLKVGQSSVAYFTVRNDMR
                     YDINDAKVELYIYSLGLSFRSIEKDIPGKDHALFRMGISVPRWVEPGEYFSRITVGND
                     RKKDVEYSYLRIV"
     gene            6224..7978
                     /gene="infB"
                     /locus_tag="VFF28_02405"
     CDS             6224..7978
                     /gene="infB"
                     /locus_tag="VFF28_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HZX44513.1"
                     /translation="MAIRSPLCVVLAHVDHGKTSILDRIRGSAIARGEAGGITQAIGA
                     SIIPLEIIKRICGKLLETLKMNFTIPGLLFIDTPGHAGFTSLRKRGGNLADIAILVID
                     INEGLKPQTVESIEILKQYKTPFVIAANKIDLVPGWQSSEKPLLQSIAGQSQNLQHVL
                     DTKLYEIVGKLAEMGFDSERFDRVDDFTKRIAIIPTSAKTGEGIPELLMILSGLAQKF
                     LEKTLKLDTSANAKGTILEVKEEKGLGKSMDVILYDGSLKKGDTIIIGTLTEPIVTKV
                     KGLFEPLPLKEMRDKKSKFSPMQEVYAATGVKISATGIDEVLAGMPLRSAKDQEIEKV
                     KQEIKEEIEEVLIHTDKEGIIIKADTLGSLEALDKLLKEKQVPIQKASLGNITKKDIS
                     DAEANYEKDPLKSVILAFSIAQEQSTDKVKIIQNNVIYKLLEDFDRWQQDEKARQEQG
                     DLHKLIMPSKFQIMRGYVFRQNNPAVVGVDVLAGTLKVGTPLMKEDGKEITEVKSIQQ
                     EQESISKAEKGKQVAVSMEGVTVGRQINEGETLYSSIPEDQFRKLKELKKHLSDEDKE
                     ILKEIAAIKRKENPVWGV"
     gene            7979..8539
                     /locus_tag="VFF28_02410"
     CDS             7979..8539
                     /locus_tag="VFF28_02410"
                     /inference="COORDINATES: protein motif:HMM:NF025020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44514.1"
                     /translation="MNLKVLDMSETGNSDFNKVSAQELPKRHRRNNYKVFRNLAVLDR
                     KDKKHFLEMLKGQFGFEEKLDYIFFINNKNKIYIVNKDLAAIDFSKLRLNSVGLYIAE
                     QRNDEARLSIEGSQMIGPLAKRNVIEITRQEAREWLKGKDLEKEVDTQGFVIIRSEDD
                     YLGCGRVSKGKILNFVPKTRRLNVSD"
     gene            complement(8522..8917)
                     /locus_tag="VFF28_02415"
     CDS             complement(8522..8917)
                     /locus_tag="VFF28_02415"
                     /inference="COORDINATES: protein motif:HMM:NF015960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF371 domain-containing protein"
                     /protein_id="HZX44515.1"
                     /translation="MEYSFTAWGHRNITGKHRRTLEFTHESELSLDGDCILGVNANFS
                     VYDLKELLADSEKLKMTISADEVSDDITFLPNKGFDGEQEIVVRMGDFSSDRTFGLKA
                     DKACADLKNELILKLKNPEQKIIILIRDI"
     gene            complement(8919..9902)
                     /locus_tag="VFF28_02420"
     CDS             complement(8919..9902)
                     /locus_tag="VFF28_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HZX44516.1"
                     /translation="MRASTVSVLFLLFISGCSTPTGRVSLESVPSEAGSLSVFFCPHD
                     DCEGALLSAVNSSKESVHCAFFDLDLPSLITLLGDKSKNIDVKLVIDEQNYGAVIGEN
                     VKKDDSSQYSHNKFCVIDGKIVTTGSMNPTNNDAHKNNNNLLVIGSRYLARNHEDEFS
                     ELWELRFGEGERVRYPEAYLNGIHIENYFCPEDKCSEKIIERLSKAEKSIYFMQFSFT
                     DEGIADAMLFNKKAEVIGIFEKRDSAGKYSQYKRLSGFGLDVFTDSNPYNMHNKVFIV
                     DNQTVITGSMNPSSAGNHKNDENILIIDDESIALEYTAEFFRLLNNASGGD"
     gene            complement(9981..11729)
                     /locus_tag="VFF28_02425"
     CDS             complement(9981..11729)
                     /locus_tag="VFF28_02425"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HZX44517.1"
                     /translation="MRMLYLCLLFLLLSASFSFAKEGHLTLLAVKESDNGYEGTTADL
                     YLELKPGTGRVFLDTFPLTKVDTQISTRFARDIACSYLDADCGKYDFVYTIRADSPIV
                     AGPSAGGAITLLTISLLKGQTISDTISVTGTINSGGIIGPVGGIKEKLDAAAQAGLKK
                     VLIPEGERLIEESFNGTNITTVDLVEYGKSKKLEVIEVSDINAAWSSFTGHKLEKDNY
                     SLTIDQDYNLTMSYLAGQLCSRSKELMGEIDSRYSKNDSMLAAVNLTRKAETALGNGR
                     YYSAASYCFGANVKLNYLLLSSRDFTKDEMRESSEVVLRNIKKLDESITRKEKKTVTD
                     LEAFSVVKERLIEAEDFYKEINSTDVYNLAYSFERVYSAYSWASFFDNRGAKYDFNTE
                     LLRSSCQRKIEEAEERFNYVKIFHPVLGLEGTRKEIDYAYSDLRNQSYELCLFKATNA
                     KAESDIILSVSGIGVDKLDNLLSRKISAAEKNIGKQIAKGNFPVLAFSYYEYAKELAT
                     YDPYSALLYAEYAIELSNLDMYFKKGKSFSFNLEMKPFVFLLIGLAFGYASAIVMRKR
                     SINPSRAKNALLGKKR"
BASE COUNT         3754 a         2572 c         2500 g         3195 t
ORIGIN      
        1 gtaaagtcaa agcagcttga agtcaagctt gaaagggtcc ttgagaggat ggaagagcag
       61 gggcaaaaca caacagatat ccagccggta atcgatagat ttaatgcaaa gatagaatct
      121 gcaaggataa gctatgagac tgcgcttggc aagtttaagc aggctataaa tgaagccgac
      181 gcagagaaag caaaagacct agctggcgaa ggcaatggat tcatgaaaga agcccaaaag
      241 tcgcttcagg aagctcaaaa ggtcctaaag gaagtcatcc agaagataaa atcagatggc
      301 ggcgagattg acgcccctga agatgcagca caggaactgg cagaggcata aaacaacaaa
      361 ctttaaaaat ctcctttcca ggaaaatgac ctgggaaggg agacaaaatt agaaaggagg
      421 caagaaaatg ctgagaaaat tggcctttat ggccttggtt tttatcgttt tggcaggctg
      481 ccagcaacaa gcagctgcgc ctgcaggtac agtggctcca actgagcaaa aagcatcctc
      541 agatttgggc gtagaagaga taaactcgct taaccaggag atagctccgc cagaaaacat
      601 cgaaacagag ctagaccagg tcaattggta agtttaatat aacctttttc tttttctttt
      661 gtttatgata agtttagacg gcgcatttct tgaaggagga ggtcagataa tcaggacagc
      721 tcttggtcta tcagcactga caggaaggcc atttgaggta aaagacatcc gtaaaggaag
      781 gcgagagcca ggcctaaaaa accagcatct ctttgggatt aaggcatttg aagagctatg
      841 cggtgcaaaa gcagaaggcg cagccttagg ttcaaccgca attacgttcg caccaggaaa
      901 gataaagccc caaaccttgt cgatagatat cggaacagca ggctctatct cactattact
      961 gcaatccctc cttatagcaa gcactttctc aaaaggaaaa ataaggctta agctcacagg
     1021 agggaccgat gtgatttgga gccctcagtt tgattatctg aacaatgtta tccttccgca
     1081 gctaagaagg tatgcagaga tagagacaag tcttcaaaag agaggatact atcccaaagg
     1141 cgcaggcaaa gtggagatta agatcaagtc agaatatggc atggaaaata taagcgaagc
     1201 gccgaagata aacctcgacc agcaatataa cctaatccag ataaaaggga tctctcatgc
     1261 atcccttgac ctgcaaaaag cagaagttgc agaaaggcag gcaaggcagg caaagcttct
     1321 tctgacaaag ttaaactgcc ctatccagat aaggacagaa tattcagaca ctctttcaac
     1381 tggctcaggc atcacactgt ggtcagtatt ctcaaaagac ccaggtgaca tcgacataaa
     1441 aaatcccatt attctcggcg cagactcgct tggagaaaga ggaaagcgtg cagaagaggt
     1501 gggtaagatt gcagcagaaa agctgataaa aggcatagaa agcaaagcgc cggtagattc
     1561 ccatctggca gataatctga tcccttatat tgcactggca aaaggaaaga taaaagtctc
     1621 agagatatcg aatcatactt tgacaaatat atatacctgc gagcagtttc ttggaaaagt
     1681 gtttactgtt gacagtgaga acaggataat tgaagcaaaa tagctcttgt tagagttttc
     1741 tgataaatca aataatcaaa gcaaaaggtt tttaaacaag gatagaatcc aaaacattat
     1801 ggaaaagcta aaatgcagtt catgcaagtc gattattaca aatcttgaag gctcgacaag
     1861 gtttgcatgc ccaagctgtg gcaagacaac gataataaga tgcaaccact gcagggacgt
     1921 aggaacaaag tatacctgtg ctgagtgcaa tttcgaaggc ccaaactaga gatggcaaat
     1981 gcagttgtta ccgtcaggat aatgccagag tcacctgata tagacttaaa ttctatagat
     2041 tcagaggcaa aaaaggttat agagtccatc gcaggcaaag gcgagaccaa atcttcacaa
     2101 attccagtcg cattcggcct taaatctttg aacattatct ttgttatgga cgaaaagaaa
     2161 ggaagccctg accctgtcgc agaaggcatc gcaaagatac ctggcgttaa ttctgctgag
     2221 atcagcgatg taagaagggc tattggatag ttattattaa gagatccagc aattctccaa
     2281 ttcttatatc cgaccttatc ccagtcccag aaaaatgcgt ctcagaagca agatatcctt
     2341 tcttcctcag cttttccaaa agctcaagct tacgaggtat ctctatcttt tccttcttag
     2401 cgattttatg caagtcataa aaaccgacag tatcgataag agattcctca gcgattatcc
     2461 gcagtatctt cgcattctca ttaactgtat tttccttggc cattttatcg gccagttttt
     2521 catcccataa ttttccaagc cacataggac cggcatccaa aaaaagccca tgctgcctta
     2581 aaacattatc agtctcttct ttgctcttat cacatctaaa gaaaaccctc atataatggt
     2641 ctttagagaa ggaaaatatt ggaaaaagcg ctttgtcgta ttgaactcct atgagctgca
     2701 ctttccttat caagatccta agcccgatct catgcataag ttcgctgcgg acaggctttg
     2761 cccaatactt tcgaaggcat gatttaggat aggtccctga aagcgcagag gtatctgtcg
     2821 cagtcacagc aagtatacct tccctgctta tcctttttac agcgcagtct aaaaaagggt
     2881 ttggagttcc aaacggatcg acatctataa agtcaaagcc gtttgaattc agcaggaata
     2941 agtttgcatc gcaattactg acagtggcat ttttgctttt caccttattc agttttaagc
     3001 tctccttaat ttttgagcag gcatttgaag aatgatcgtt gacaccaagt gaggaaatct
     3061 tacctgtctt cagctctaaa agcagcctga cacttctcac ccccgaacca gcaagaggat
     3121 cagcgatgtt catattccat ttatctattg tattcaagag caatactgta acatctctat
     3181 tgaacttcat cacaggatta taaaaaacag gcatctgttt gctgactatc ttgacgacag
     3241 gcactttgag tttagccatc ccttcctgca taagtttata catagcttta aaaaataata
     3301 gataagataa aaatgtttgc ccaattcagg cagaaaaagt ataaaatatg cctcaacgga
     3361 aagcatatgt gatctttctt tcccatatat tgcaaatacc tcatatatag tatatggctc
     3421 agctatatct agatataaag cgcttattag cttatgaggt gatatcttat gggcgaagac
     3481 tgggactttg gacagccttc tgttgatgca agccttgatg agctcatcgc agagcttagg
     3541 acgcataaaa agtgcataaa aagcgctgcc atcggaagca agcagtataa aacgcttgat
     3601 cttgtcatag aggcatataa agcccatccg ctgcaagtgc ctgacaagct agtataccat
     3661 ctccttgagc tggatatcat tccccttatt gatttcgagg gaaggtatta ctttgctgtg
     3721 atgtatgaag ggaaaagtgc tggcactacc cgatcgtcca gcaatattat cgtcaactta
     3781 gaggataatc tttttgcaag ggactcaaga tttaattacg ataacgcacg acttatcctg
     3841 cttaaacgcg aggaatatca agctgtctat caggcagttc agggagggcc ggtcgaacca
     3901 gcccctagga cagaggtaaa gaagcctctc gtccagttag acccaatcca taagctcatc
     3961 tacaagatag tctcgactgc tgttgcaaag agagcgacag acatacattt tgaaagggtc
     4021 ggaactgata atacaaggat aagatatagg atagacgggc atcttgtcgt ttatgacgat
     4081 tccatagagc ctgacctgat gagaagggtc acatcagcga taaagactat ggcagaggtc
     4141 aaatcttatg atcctcttga tccacaggat ggagtgataa ccttcaagcc tcagatagtt
     4201 gaactttttc caaacctaaa ggactattcg ctcaggcttt cgacagtgcc tgtcaaatgc
     4261 gacgcagcga caaaggaagt ggattgtctt gcgctaaggc ttcatgaagc agagatagga
     4321 aggcacactc ttggcgagct tggatttccg ccagaagttg ttagacaaat ctatagtgtc
     4381 atctccgcat ctcaggggtt gatgctggtc acagggccta ctggttcagg caagacaaca
     4441 acgctttatt cgatattaga tcacctaaac aagcctgaga gaaacattct tacaatcgag
     4501 gatccgatag aagcatttat ccctggatta aaccaaagcc aggtaaacaa agaaagagga
     4561 tggacatttg aaaaagtgat gaggcattat ctcaggcaag atccaaatgt catacttgtt
     4621 ggagagacaa gggatgaaga gacagcaaaa cttctatttt cagctgcaga gacaggccac
     4681 tttgtattca gcacagtgca tacgaacagc gcatatgata ctttaacaag ggtatttgaa
     4741 ctgggtattg cgcctactta tgtagtgtca tcccttagcg ctgtactttc acagcgtctt
     4801 gcaaggctcc tttgtacaga ttgcaaaaaa gattatgacg caacagccca gattgaagag
     4861 atattcagga cacaattaag ccatcctctt gtattataca ggccaggaga catagattgc
     4921 aaaagatgcc acggcacagg atattatgga agaattgctg ttcctgagct atgggtgctt
     4981 gatgaggaag caagggatat ggtcgcagcc ggggagaagt acacatcaag atacaggcag
     5041 gctgcacaaa agaaaggggc aaaaacactg atgcagaacg gccttgagct tgtgcttgca
     5101 ggaaagacat ctcttcctga gatagcaagg ctctcgcaaa gcgggcttta ttatgaaaag
     5161 gcgtatctgt tgccgactgt cctagaaagg cagaaaggat aattttttta tgctttttgt
     5221 agagtctagc cacctctttt aaatgcatgg tacacttttt caggatcgat atttttcatc
     5281 ataacctttg caaatttacc aagggcttgg ttaacctttc catgtatttc agtagaaata
     5341 tcattgattt tttctttctc ttgcatttct ttctctgtga aacttagtgc atatctaatc
     5401 agcaagcatc ccccgacata ttgtatatca tttacgtgcc gctcagtagt atttccgact
     5461 gcattattta cagcttcttt catagcatca actttatcct caagaccttt accgacaagg
     5521 taacccgaat agaaataacc tttgtttttg ttgcttcttg acaaagtgac tacgaatggg
     5581 tatcttgggt caggatggcc ataatgatct cccattagta caaagttgtt cttttctata
     5641 agcgatttct caagtcccaa gcattctgat ttttgagccc taggaattgt atggacagca
     5701 tactgcaaat aacttgctct tctttggtca tatctcatgc agatccctca gacaatcctc
     5761 aaataactat actccacatc cttcttcctg tcattgccta cagttatcct agaaaaatac
     5821 tctcctggct ctacccatcg tggaacagat atccccatcc taaagagagc atggtctttt
     5881 ccagggatgt ctttctctat tgacctaaaa gaaagcccca gggaatagat ataaagttca
     5941 acctttgcat cattgatatc atacctcata tcattacgga cagtaaagta agcaacactg
     6001 ctctggccta ccttaagagg ctgctggata tagtaccttc caagaattgg gctcaccaca
     6061 aaaagaccta gcaaaaacaa tgccaaccat ttcataatat caccctttta gtaatcgttc
     6121 cttaaccatt taactatata aacttttatt gtctccagtt gcggacgata tgattagcag
     6181 cccaatcata acgtttaaaa agcataataa attagtttca ggaatggcga taagaagtcc
     6241 tctatgtgtt gttttggcgc acgttgatca cggaaagaca agtatcttag acaggatcag
     6301 aggcagcgct atagcaagag gagaagcagg aggcataact caagctatag gagcatcgat
     6361 catcccctta gagatcatta aaaggatctg cggaaagctc ttagaaacat taaagatgaa
     6421 ctttaccatt ccgggtctgt tgtttatcga cactccaggc catgcaggat ttacgagcct
     6481 aagaaaaagg ggcggaaacc tggcagatat tgcgatcctg gttattgaca tcaatgaagg
     6541 gctaaaaccc cagactgtag aatcgattga gatcctaaag caatacaaga ctccttttgt
     6601 cattgcagca aacaagatag atcttgttcc tggttggcaa tcatcagaaa agccgctact
     6661 ccaaagtatt gcaggccaaa gccagaatct ccaacacgta ttagacacaa aactgtatga
     6721 aatagttgga aagcttgcag agatgggttt tgattccgag cgctttgata gggttgatga
     6781 tttcacaaag cgcattgcaa tcatcccaac atcagcaaag acaggagagg gtattccaga
     6841 gcttttgatg atactctctg gactggccca aaaatttctg gaaaagaccc tgaagcttga
     6901 cacaagtgca aatgcaaaag gcacaattct cgaggtaaag gaagaaaaag gccttggaaa
     6961 atcgatggat gtcatccttt acgatggcag cctaaaaaaa ggcgatacca taatcatagg
     7021 aactctgaca gagccgatag tcacaaaagt aaaaggcttg tttgagcccc ttcctctaaa
     7081 agagatgaga gacaaaaaaa gcaaattttc tcctatgcag gaagtttacg cagcaacagg
     7141 cgtcaagata tccgcaacag gcatagacga agtccttgca ggcatgccgt taaggtcagc
     7201 aaaagaccaa gagatagaaa aagtaaagca ggaaataaaa gaagagatag aagaagtcct
     7261 catacataca gacaaagagg gcataatcat aaaggcagac accttaggct cattagaggc
     7321 attagacaaa ctcctaaaag aaaagcaagt gccaattcaa aaagcatccc tgggaaatat
     7381 caccaaaaaa gatatatctg atgcagaggc aaactatgaa aaagatcctc taaagtcagt
     7441 catccttgca ttcagcatag cccaagaaca atcgacagac aaggtaaaga taatccaaaa
     7501 caacgtcatc tataagcttc ttgaagactt tgataggtgg cagcaggatg aaaaggcaag
     7561 gcaggagcaa ggggatctcc acaagttgat aatgccaagc aaattccaga ttatgagagg
     7621 ctatgttttc aggcaaaaca atccggcagt cgttggtgtt gatgttcttg ctggaactct
     7681 gaaagtagga acccccctta tgaaagaaga tggcaaggag ataacagaag taaaaagcat
     7741 ccagcaggag caggaaagca taagcaaggc agagaaagga aaacaggtcg ctgtctccat
     7801 ggaaggtgta acggttggca ggcagataaa tgagggagaa acattatact caagcattcc
     7861 agaagatcag tttagaaagc taaaagagct aaaaaaacac ctgtcagacg aagacaagga
     7921 gatcctaaaa gagatagcag cgataaaaag aaaggaaaat cctgtctggg gtgtttgaat
     7981 gaaccttaaa gttctagaca tgtcagaaac cggaaattca gattttaata aggtttctgc
     8041 tcaagaactg cctaagaggc accggcgaaa taattacaaa gtgtttcgta acctggcggt
     8101 tcttgacaga aaagacaaga agcattttct tgaaatgtta aaaggccagt ttggctttga
     8161 agaaaagctt gactatatct tttttatcaa caataaaaat aagatataca tcgtaaacaa
     8221 agatttagcg gcaatagatt tcagcaagct taggctgaat tctgtcggcc tatacatcgc
     8281 tgagcaaagg aatgacgaag caagactttc gatagaaggc tctcagatga tcggcccatt
     8341 agcaaaaagg aatgtgatag agataacaag gcaggaagca agagaatggc ttaaaggaaa
     8401 agacctggaa aaagaggtag acacccaagg ctttgtcata ataagatcag aagacgacta
     8461 tcttggctgc ggcagggtct caaaaggaaa gatcctgaac tttgttccaa agacaaggcg
     8521 tttaaatgtc tctgattaag attattatct tctgctcagg attctttaat tttaagataa
     8581 gctcattttt tagatcagcg catgccttgt ctgccttgag cccaaaggtg cggtcagatg
     8641 aaaaatcccc catacgcacc acaatctcct gctcgccgtc aaaacctttg ttcggcaaaa
     8701 aagtgatatc atcactgact tcatcggcgc tgattgtcat cttcagcttc tcgctgtcag
     8761 ccaaaagctc tttgaggtca taaacgctga aatttgcgtt cacccccagt atgcagtctc
     8821 catcaaggct gagctcgctc tcatgggtaa attcaagagt ccgtctatgc tttcctgtga
     8881 tgttcctatg tccccatgct gtaaatgaat attccatctt agtctcctcc gcttgcgtta
     8941 tttaataatc taaaaaactc agcagtatat tccagagcta tggattcatc atcgattatt
     9001 aaaatattct catcattttt atggttgcca gcagagctag gattcatcga gccggtgatg
     9061 actgtctggt tatcaacaat aaacaccttg ttgtgcatgt tataaggatt actgtcagta
     9121 aaaacatcca gcccaaagcc tgatagccgc ttgtactgcg aatatttgcc agctgaatcc
     9181 cttttctcaa atatccctat cacttcagct tttttattaa atagcattgc atcagcgatt
     9241 ccctcatcag taaatgaaaa ttgcatgaaa taaatgcttt tctcagcctt ggacagccgc
     9301 tcgattatct tctcagagca tttgtcctca gggcagaaat agttctcgat atgtattcca
     9361 ttaagatacg cctctggata ccttactctt tccccctcgc caaacctaag ctcccacagt
     9421 tcagagaact catcctcatg gttccttgca agataccttg atcctatcac aagcaggttg
     9481 ttattattct tatgagcatc attgttagta gggttcatgc tccctgttgt tacaatcttt
     9541 ccgtcgataa cacagaactt gttgtgtgag tactggctgc tgtcatcctt ttttacattt
     9601 tcaccgatta cggctccata gttctgttca tctatgacca gcttgacatc tatattcttg
     9661 cttttatcgc caagcaaagt aatcaggctg ggcaggtcaa ggtcaaaaaa agcgcaatgt
     9721 acagactcct ttgaagagtt tacagcagaa agaagtgctc cttcgcagtc atcatgcggg
     9781 caaaaaaaca ccgatagaga gccagcctca gaaggtacgc tctcaaggct caccctgcct
     9841 gtaggcgtag agcagccaga tataaacaaa agaaacaata cgctaactgt acttgccctc
     9901 attttgtcac cactctagga taatattacg gggaaaggtt taaataatag ctgtactatt
     9961 tttagcttga tagtaatcta tcacctcttt tttcccagca gggcattttt tgctctgctc
    10021 gggtttatgc tccttttcct catcactatt gcgcttgcat atccaaaagc aagccctatc
    10081 aatagaaaaa caaagggttt catctccaga ttgaatgaaa aacttttccc cttcttgaag
    10141 tacatgtcaa ggttgctgag ttctattgca tactcagcat aaagcagtgc agaatatgga
    10201 tcataggttg caagctcttt tgcatactca tagtaagaga atgcaagcac agggaaattt
    10261 ccctttgcta tctgtttgcc tatatttttc tcagcagcgc ttattttcct gctaaggagg
    10321 ttgtccagtt tgtctacccc tatgccactg acgcttaaga tgatgtcaga ctcagctttt
    10381 gcgtttgtcg ccttaaaaag gcacagctca tagctttggt tcctaaggtc actatatgca
    10441 tagtctatct cttttcttgt gccttcaagg cccagcacag ggtgaaatat cttgacatag
    10501 ttaaacctct cctctgcttc ctctatcttt ctttggcagg aagacctaag aagttcagta
    10561 ttaaagtcat acttagctcc tctgttgtca aaaaaactag cccatgaata agcagaatat
    10621 accctttcaa acgagtaggc aagattgtag acatctgttg agtttatctc tttgtaaaaa
    10681 tcctctgcct cgataagtct ttctttgact acagaaaacg cctcaaggtc ggtcactgtc
    10741 ttcttttcct ttcttgttat gctttcgtca agttttttta tgtttctcaa aacgacctct
    10801 gagctttccc tcatctcgtc tttagtgaaa tcccttgaag acaaaagcag atagttaagc
    10861 tttacatttg ccccaaaaca ataagacgca gccgaataat accttccgtt accaagcgca
    10921 gtttctgctt tcctggtaag gttcacggca gcaagcatcg agtcgttttt gctgtatctt
    10981 gagtctatct cacccataag ctctttgctc ctgctgcaaa gctgcccggc aagatatgac
    11041 atggttagat tatagtcctg gtctattgtt aaggaataat tatccttttc cagtttatgc
    11101 cctgtaaaag aagaccatgc cgcatttata tcggaaacct ctattacctc caatttcttg
    11161 ctcttcccat attcaaccag gtcaacagtg gtgatgttgg tgccgttgaa actctcttcg
    11221 ataagccttt ccccttcagg tatgagcact ttcttaaggc cagcttgtgc agcagcatct
    11281 agcttttctt ttatgccgcc cactggcccg attataccgc ctgagtttat cgtgcctgta
    11341 acacttatag tatccgatat tgtctggcct ttgaggagag atattgtcaa aagggtgata
    11401 gctcctccag cagatggtcc tgcgacgata ggagagtcag ctcttatcgt atatacaaaa
    11461 tcatatttcc cgcagtccgc gtctagataa gagcatgcta tatcccttgc aaaccttgtg
    11521 gatatctgtg tgtctacttt tgtcagcgga aatgtatcaa gaaacaccct tcctgtcccg
    11581 ggttttagct caagataaag atcagcggtc gttccctcat agccattgtc cgattccttc
    11641 acagcaagaa gggtaagatg accttccttc gcgaagctaa aactggcaga caatagcagg
    11701 aatagtaagc acaagtaaag catcctcatg catagggaga atgaatatgc ttttttatta
    11761 ctttctaatt tgtgtatgta ttaagctaac aagggggact ctactgtcct aaggtcaaaa
    11821 ctccatccaa taacaattat acagctcgtc gtagggggtc gccccagccg gggatacaga
    11881 acctttgaga tgtcagaaac caccactcgc aatctccctg cttcagctcg gagtaagaag
    11941 gtggtttctg agcatgctca agaacacgca ccgaggctct tcagcccggc ggttcttgac
    12001 aaacaaatca agagttttga a
//
LOCUS       DASRUK010000051         4468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2050629, whole genome shotgun sequence.
ACCESSION   DASRUK010000051 DASRUK010000000
VERSION     DASRUK010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2050629"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1198)
                     /gene="thrC"
                     /locus_tag="VFF28_02430"
     CDS             complement(<1..1198)
                     /gene="thrC"
                     /locus_tag="VFF28_02430"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZX44518.1"
                     /translation="MAMLFRSTNRNSPSVGFKEALMQGQAPDYGLYMPGTIPQFTKQE
                     IASFQEKSYPEIAFEIAKKYVDKIDDSKLRKLVDDAYDFEVPIERLNDDLYIMRLDRG
                     PTASFKDFAARLMARLMHYFAEKENQKLTILVATSGDTGGAVADAFYGLKNINVIVLF
                     PEKEVSDRQRKQMTTLGKNVKAIAVKGKFDDCQALVKRAFNDPELKSLNLTSANSINF
                     GRLMPQMVYYFYAQSRIKGKVNFSVPSGNFGDLMGGVFAREMGLPVNKFIVAVNGNDE
                     FPKFLAAGQYAPVVPSKECSSNAMNVGHPSNLARLIDLYGGWLTDERDSNGIVAKKGF
                     LREKPDMEKMRKDFVSFSITDQEVDEAIKKVSENYSAIIEPHGAVGIAAALKSKIKGK
                     IICLETA"
     gene            complement(1238..1942)
                     /locus_tag="VFF28_02435"
                     /pseudo
     CDS             complement(1238..1942)
                     /locus_tag="VFF28_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
     gene            complement(1966..2985)
                     /locus_tag="VFF28_02440"
     CDS             complement(1966..2985)
                     /locus_tag="VFF28_02440"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HZX44519.1"
                     /translation="MTMVYQVIALIVLLALSAFFSASETALISISKLSVRHLVKLKKR
                     GARELQLLKDEPNRIIITILIGNNIVNVTISALVTIITLEIFGNDAIGYAVGLTTFIL
                     LLFGEITPKSFASHYSRRLSLLFSKPVYYLSIAFYPIIKVLDIFTRLFIRIFGWKEKK
                     QGITKEEIETMIEIGEASGALKRMEEMLVKNVFRFDDIEVEEIMTPKDKMFSLEAETT
                     IKDAIPVLLQRPFTRVPIYMDDPENILGFISRDDILRAYANHKESIPLRLILKPIPCI
                     FRDEKLDVLLNQFQRKHIHIAAVITRNHKLLGIVTLEDLLEELVGEIIDEKEIEALKL
                     QESLK"
     gene            3105..4406
                     /locus_tag="VFF28_02445"
     CDS             3105..4406
                     /locus_tag="VFF28_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44520.1"
                     /translation="MKKIIIILAAVAVLTVSILSACYYDDEYIDKDVDNGVQQDNGIE
                     EVVDWQASDEGQGSGYSVTPEDDDIIGIDEKDDTQNVETKIGDIYGGDKEDDQQIIES
                     KVVDVYEENLTSQIYNENRTGETGNDTIITENLTSSAENKNPNRAKGQGIFSVVNTLN
                     VASHDNNPVTQVSGVFTTNKTDSNDIYLVPYVGDFDGNIDSDWYHFYHKLIKWHEETG
                     IKTGISFYPSSLDNTEFKDIIAKASGPNMELIMKSEASYKGKRLAEMDKDEAKEAIGA
                     LLDKCKEKLGIIPTTYNQELAEFTPEIRDAVRELGFKMYLEHYESDYGYVDMTPDFDI
                     TQYSVSFTKNGLAGPENEFKTADDMISEIMSYEHEMMITIDGKRLIPLLVHQQDFRAS
                     EESDELNEKKWKTYTETLTKASKDPRIRILLPSEVYGMRHK"
BASE COUNT         1281 a          903 c          916 g         1368 t
ORIGIN      
        1 ctgctgtttc taggcagatt atcttacctt ttatttttga ctttagggct gctgcgatac
       61 cgacggcacc gtgcggttcg attattgcac tgtagttttc gctgactttt tttatagcct
      121 catcgacctc ttgatcagtg atggaaaaac tgacgaaatc cttcctcatc ttctccatat
      181 caggcttttc tcttaaaaaa cctttcttag caacaatacc atttgagtct ctctcatctg
      241 tgagccaccc accatacagg tctatcaacc tcgccaggtt gcttggatgg ccgacattca
      301 tggcgtttga actgcactct tttgaaggaa ctacaggagc atattgacct gctgccaaaa
      361 atttagggaa ttcgtcattt ccattgactg cgacaataaa tttgttaaca ggcaagccca
      421 tctctcttgc aaagacgccg cccattaagt ccccaaagtt acctgagggc actgagaagt
      481 ttactttgcc ctttattctt gattgagcgt aaaagtaata caccatctga ggcataagtc
      541 gtccgaaatt tatcgaattg gcagatgtca ggtttaggga ttttaattcg ggatcattga
      601 aggccctttt taccaaggcc tggcaatcgt cgaattttcc tttgactgct atggctttta
      661 cattcttgcc aagggttgtc atctgctttc tttgcctatc agagacttcc ttttccggga
      721 agaggacgat gacattaata ttctttaggc cataaaaggc atctgcaaca gctcctcctg
      781 tgtcgcccga agttgctaca aggatggtca gcttttgatt ttctttctct gcgaagtagt
      841 gcatcaacct tgccataagc cttgctgcaa agtccttaaa agacgctgtc gggcctctat
      901 ctaagcgcat tatatacaaa tcatcattca atctctcaat tggcacttca aaatcatagg
      961 catcgtctac cagttttctt agctttgaat catctatctt atcgacatat tttttagcga
     1021 tttcaaaagc aatttcagga taactcttct cttgaaatga cgctatctcc tgctttgtaa
     1081 actgggggat agtcccaggc atataaagcc cataatcagg tgcttgcccc tgcataaggg
     1141 cttctttgaa gcctacgctg ggagaatttc ggttagtgct tctaaatagc atagccatcc
     1201 atgctgaaat taggctttat tttaaaaaat tagtggtcta gcaaagctcg actattatct
     1261 ttgccgctgc cttaactgtc agattattga tatcgtgctc aggattgact tctgcaatat
     1321 ccatagctct tagattcctt aatctcttta gcctatgaag gaagaacaac agctctcttg
     1381 cagacaagcc tcctggctca ggtcaccctg tacctggagc ataggctggg tcaaggacgt
     1441 ctatatctat gcttatgtag agatccgaga aattctttgc tatcgacata aagctgtcag
     1501 atacctctct tagtccctca gatgtaatct ctttcatgga aaagagccta atattgtgtt
     1561 cttttgcaaa ctgaagctcc ttttggccga atcttctgca tcctaccagc acgagatttt
     1621 caccattgaa gatgctcact aaatcattaa gaatatcttt gtcgaattca gcatcgatat
     1681 gagcgtcaaa aaagatgact cctggatttt ttggatatac attattgaac gctctcaaga
     1741 taggcaagct tgaagcatgc tttgagcaga gaaacaaagg tttacttgct gccaaaaatt
     1801 ccagtgcttt ttcctctata tcttcctctt tgatgctcac tatctcgata acgggataga
     1861 tgccttcctc agaagaatca tagcgcttta tctcttcaat tattgcttca ggcacttggc
     1921 tgccaacctt gatcagcttc attgtccttt tagaagtaac cttgcttatt ttaaactttc
     1981 ctgcagcttt agcgcttcta tctctttttc gtctattatc tcacccacaa gctcttcaag
     2041 aaggtcttct aaagtgacta ttccaagaag cttgtggttc cttgttataa ctgcagcaat
     2101 gtggatatgc ttcctttgga attggtttaa aaggacatcc agcttttcat ccctgaagat
     2161 gcaggggatg ggttttagta tcagccttaa ggggatggac tccttgtggt ttgcatatgc
     2221 cctgaggata tcatccctgc tgatgaatcc caggatgttt tcaggatcgt ccatatatat
     2281 cggtactctt gtgaatggcc tctgaagcag tacaggaatc gcatccttga tagtcgtctc
     2341 tgcttccagg ctaaacatct tatccttagg agtcattatc tcttcaactt caatatcatc
     2401 aaagcgaaag acgtttttta ccagcatctc ttccatcctt ttcaacgctc cgctggcttc
     2461 accaatctct atcattgtct ctatctcctc ctttgttata ccttgttttt tttctttcca
     2521 gccgaatata cggatgaata gtctggtaaa aatatcaagg actttgatga tgggataaaa
     2581 agcgatgctc aaataatata ccggcttaga gaataagagc gacagccttc ggctgtaatg
     2641 gcttgcaaag gattttggcg ttatctctcc gaaaagcagc aggataaatg ttgtcaagcc
     2701 aactgcataa ccaatggcat cattgccaaa aatctcaagg gtgattattg ttacaagagc
     2761 tgagatcgtg acatttacaa tattgtttcc gatgagtata gtgataatta tcctgttcgg
     2821 ttcgtctttt aataactgca gctcccttgc acctcttttc ttgagcttga ccagatgcct
     2881 tacactaagc ttgcttatgg agatcaaagc tgtctctgat gctgagaaga acgctgacag
     2941 tgcaagaagc actatcaatg ctattacctg atataccatg gtcatgataa gtaggctaaa
     3001 aaagtggatt taaaaatgtt attcttattg gtgattagta tttctggata attataaaat
     3061 aattaatatt tccgaaacct tgggattcgc ttgggggtgg gaaaatgaag aaaataataa
     3121 ttattcttgc agcagttgct gttctgacag tgtctatatt gagcgcttgc tattatgacg
     3181 atgaatatat tgacaaagat gtggataatg gtgttcaaca agataatgga attgaagaag
     3241 ttgttgactg gcaagcaagt gacgaggggc aaggctcggg ttattcagtg acacctgaag
     3301 acgacgacat tatagggatt gatgaaaagg atgatacaca gaatgttgaa accaaaatag
     3361 gtgatatata tggtggcgat aaagaagatg atcaacaaat aattgaatct aaagtagtcg
     3421 atgtgtatga ggaaaatctt acaagtcaaa tttacaatga aaataggaca ggcgagacag
     3481 gtaatgacac aataattact gagaatctca cctcatcagc cgaaaataaa aatcccaacc
     3541 gcgccaaggg gcagggtatt tttagtgttg taaataccct caacgtggcg agccatgaca
     3601 ataacccagt cacgcaagtg agcggggtat ttacaacaaa taaaacagat agtaatgata
     3661 tctatcttgt tccttatgtc ggtgattttg acggaaatat agatagcgac tggtatcatt
     3721 tttatcacaa gcttatcaaa tggcatgagg aaactggcat taaaacagga ataagcttct
     3781 acccatcttc tctggataat acagagttta aggacataat tgctaaagcc tctggtccca
     3841 atatggagct tataatgaag tctgaagcgt cgtataaagg aaaacggctt gctgagatgg
     3901 acaaggatga agctaaagag gcgatcgggg ctcttttgga caaatgcaaa gaaaaacttg
     3961 gcataatccc tactacatac aaccaggagc ttgctgaatt tactccagag ataagagatg
     4021 ctgttcgaga gctggggttt aaaatgtatc ttgagcacta tgagagcgat tatgggtatg
     4081 tggatatgac gcctgatttt gatatcacgc agtatagcgt aagttttacc aaaaatgggc
     4141 tagctggccc tgagaatgag ttcaagacag ctgatgatat gatttccgag ataatgagtt
     4201 atgagcatga aatgatgatc actatcgatg gcaaacggtt aataccattg cttgtgcacc
     4261 agcaggactt tagggcctct gaggagagcg atgagttaaa tgaaaagaaa tggaagactt
     4321 atacagaaac tttaaccaaa gcaagcaaag acccaaggat acgcatattg ctgccgagtg
     4381 aggtgtatgg gatgaggcat aaataatttg ttttaatttt attcatattt ataatcaagg
     4441 tttttgatcg cgattgcaag catgtcct
//
LOCUS       DASRUK010000052         8965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_709684, whole genome shotgun sequence.
ACCESSION   DASRUK010000052 DASRUK010000000
VERSION     DASRUK010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 8965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8965
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709684"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="VFF28_02450"
     CDS             <1..1063
                     /locus_tag="VFF28_02450"
                     /inference="COORDINATES: protein motif:HMM:NF013601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HZX44521.1"
                     /translation="TMKLFDSSPGCIPKYEHVLIDEYQDVNDMQIKLIEQLNPKNIFA
                     VGDPRQSIFGWRGANIEHIKRFKEKHPESKILGLRINYRSKQSIIDVLNSVSSPMRMP
                     EITCCDKNAASDKDVILLEHDNEEAEHMFVVQSILNLSMRKDVFVLARTNKQIEKIAE
                     KLKAAGIDFVKRTIEENGGEAKETQITLSTVHAIKGLEAEIVYLISANSFSFPCRASE
                     HPILDILKTQSYDKYEEELRLLYVALSRAKSKLIISYYSSLSPFIDDKTKKLIGGREE
                     APKKGKPSLYDVLKRWRLEKSRELGVAAFQIFSDKTIMEICQTGPGTIEELRDVYGMG
                     PNKIMRFGKDIVRMVIEND"
     gene            complement(1064..1648)
                     /locus_tag="VFF28_02455"
     CDS             complement(1064..1648)
                     /locus_tag="VFF28_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44522.1"
                     /translation="MYQPAYNDKIALGLARPISVGYGESGDGKEFSVCINPFGTKRNF
                     YTHKSLFGAYPLSYPDKDSRIRLAAYRSFGGTIEDFIELDNCRIIHGNGLALPKNMKV
                     HESYHGFPRGLELALKTPYSLRKTALDIRRKNNLKPTLGSFHDVNPSPKRVSQRDYLY
                     FADDESDFRLIAYSLFAGIDRSIIARALAAYKAR"
     gene            complement(1742..2593)
                     /locus_tag="VFF28_02460"
     CDS             complement(1742..2593)
                     /locus_tag="VFF28_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011460068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HZX44523.1"
                     /translation="MAIGNQLKTALLLGILTGLLLLIGSFWGQSGLLIALIFSILLNF
                     IAYWFSDKIALAMYRAKAVTEKEELILYKAVKEVSSLAKIPTPRVFVIPAPYANAFAT
                     GRNPKHSAVAATRGILDLLNEEELKGVIAHEVSHIKNRDILISSVAAVIAGVISYIAM
                     MARWSAIFGYGRDEEGNSGLELLVFAILTPIIAAIIRLAISRSREYLADASGARIVGS
                     GEGLASALLKLEQSAKHISLAPTPKAQATSHLFISNPFRGRSFLSFFMTHPPVEKRVE
                     RLRSQKF"
     gene            2829..3191
                     /locus_tag="VFF28_02465"
     CDS             2829..3191
                     /locus_tag="VFF28_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44524.1"
                     /translation="MAANTTKKLGVWAFIFGVILALIVGLLAANLTAYMGLISALMVL
                     LGLIVGILNITEEEVNSFIIAAIGLATGSIAIANLGTLLPGTVGVMLKNTFSVFSVFV
                     AGAVFIPALKSVYRISRD"
     gene            complement(3160..3555)
                     /locus_tag="VFF28_02470"
     CDS             complement(3160..3555)
                     /locus_tag="VFF28_02470"
                     /inference="COORDINATES: protein motif:HMM:NF025044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HZX44525.1"
                     /translation="MEEIELLLDWIERLREGERIIVVEGKEDKAALHRLGIDGVIQLN
                     RRPLFKIVEELVAKERPVIILTDLDKKGKQLYSKLNHDLNQFGVRIDNRFRHFLFRHT
                     QLRQIEGLDSYLERKNKKGFNPLRFCILT"
     gene            3603..4916
                     /locus_tag="VFF28_02475"
     CDS             3603..4916
                     /locus_tag="VFF28_02475"
                     /inference="COORDINATES: protein motif:HMM:NF016030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44526.1"
                     /translation="MITLSETLKHYKQPDIQEEIVLAAKDKEVAIKFGDKGFGKRPDA
                     LKYPNDVLELAKQGATSFHSSEELWSNPLQLDPMMKKKDMEDLRIGWDLVIDIDCKFL
                     DYSQITADLLIKALKFQGINSVSCKFSGGSGFHIGVPFEAFPKKVHDREARLWFPDGA
                     RAIALYLKEMIRKPLSERMLAYENPDKIMAKTGKPFNEIIKKGIFDAFAVVGIDTILI
                     SSRHLCRMPYSFNEKSGLVSVPIDPEKVMEFRREDAASTKVSVSKFRFLDRSFAMENE
                     AKKLLVQAFDFNLKLEEEKPKKEYEIPQEAIPEKFFPPCINNILKGVDDGKKRSMFIL
                     TNFLNSVGWSYEQMEKSLDEWNKKNKEPLREVLLKGQLRYHKQRAKKILPPNCSNSMY
                     YKDFRVCTPDNLCHRIRNPVNYARAKTRYLEKAKKPEKAQKSTNT"
     gene            5103..6071
                     /locus_tag="VFF28_02480"
     CDS             5103..6071
                     /locus_tag="VFF28_02480"
                     /inference="COORDINATES: protein motif:HMM:NF003261.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HZX44527.1"
                     /translation="MPATRRPRFGSLAFYPRKRAKRETARVRAWAKSKEPKLLGFAGY
                     KVGMAHVMATDNSPTALTKGQTISIPCTIIECPSLKAYSIRFYKNTIYGQKVICDVLA
                     ENLNKELARRTIMPKNRKSKVPDSKDYDDVMLAVYSQPGLSGIGKKKPEIFEIGIGGA
                     KKEDKLKYAQELIAKEISVKDVFAEGNQLDIHAISKGKGLQGPVARFGIGLKNHKNEK
                     TRRNPGSLGPWCGQVNIMWKVPHAGQTGYFQRMDHNKWLLKIGAKGEELSPKGGFMHY
                     GFIKNPYIIVKGSVTGPKKRLIRFNQAIRPNKGLPKEAPAIKQVVL"
     gene            6068..6877
                     /gene="rpl4p"
                     /locus_tag="VFF28_02485"
     CDS             6068..6877
                     /gene="rpl4p"
                     /locus_tag="VFF28_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HZX44528.1"
                     /translation="MKEAKVVDIENNEKGKVKIPSQFSEEIRPDLIKRAYITLMSHTR
                     QRYGATPMAGKRASAIVSRQRHDYRGAYGLGISRVPRKIMSRRGTRMNWVGAFAPGTV
                     GGRRAHPPKAEKIWWQKINRKERKKAIRSAMAATLDKEIVSKRGHIIGENYPIIVDSS
                     FEDIQKTKQVEQMLVKMGFENELSRCSKKKIRAGVGKTRGRKYKQKKGPLFVVSKSCK
                     LIKAAANIPGVDVCEVKNVNVRLLAPGTVPGRLTVWSKAAIEIIEKEKLFL"
     gene            6879..7133
                     /locus_tag="VFF28_02490"
     CDS             6879..7133
                     /locus_tag="VFF28_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014122408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HZX44529.1"
                     /translation="MENVIKNPLSTEKAIRLMESENKLLFVIDRKATKKDVKEAIENM
                     FKAKVVAVNTLVGPDGVKRAYVKFSAETPAIDIATQLGLM"
     gene            7137..7853
                     /locus_tag="VFF28_02495"
     CDS             7137..7853
                     /locus_tag="VFF28_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HZX44530.1"
                     /translation="MGKNLIQQKRGKGSSTYRAPSFKYVGRAKYGTYGNLPSKGTITD
                     IIHCPGHSAPLISVKYDNGSDVLLQAAEGLKVGSEVATGDGASLMPGNVLALKDIPEG
                     TDVFNIESMPGDGGKFVRASGTMAKVIAKSNDKIILILPSKKQRNFSPMCRATIGIIA
                     GAGKKEKPFLKAGFKMHAMRARNKIYPKVAGLAMNAVDHPFGGSSTNAKGKCSTVSRD
                     APPGRKVGKIAARRTGRKRR"
     gene            7855..8238
                     /locus_tag="VFF28_02500"
     CDS             7855..8238
                     /locus_tag="VFF28_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012571027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HZX44531.1"
                     /translation="MARKEFSYRGKSVSELKSLSLNEYAQIAPARIRRSIKRGFTEQQ
                     KILLANIRAKKSNIETHCKDMIILPEMIGMMIKIHNGKEFLPVTIGNEMIGHYLGEFV
                     STRKRVEHSAPGIGATKSSAALSVK"
     gene            8240..8707
                     /gene="rplV"
                     /locus_tag="VFF28_02505"
     CDS             8240..8707
                     /gene="rplV"
                     /locus_tag="VFF28_02505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HZX44532.1"
                     /translation="MAKYSTVDYEKENMARAIGRSLSISTKASVEICSFVRDRSTTEA
                     KKLLSEVIELERAVPYRVYNKGLAHKRKIGPGKYPAKAAGEVIRVIESAEANAQFKGL
                     NTANLIIKHINAHLAAKQWHYGRKRRRRAKRTHIEIIVQEKKPEKAKEKKAKK"
     gene            8704..>8965
                     /locus_tag="VFF28_02510"
     CDS             8704..>8965
                     /locus_tag="VFF28_02510"
                     /inference="COORDINATES: protein motif:HMM:NF019275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HZX44533.1"
                     /translation="MIERKFVNEKIKEFQIQEYVRSSLRNAGLSHIKVQRTPLGEKII
                     VYTSRPGLVVGRKGQNIKVLTKTLKKEFSLENPQIEIAEVGNI"
BASE COUNT         3030 a         1706 c         2057 g         2172 t
ORIGIN      
        1 tacgatgaaa ctgtttgatt catcccctgg atgcatacct aagtatgaac atgtgctcat
       61 agatgaatac caggatgtaa acgacatgca gataaaactg atagagcaac taaatcctaa
      121 aaatattttt gcggtgggcg atcccagaca gagcatattt ggatggagag gagcaaatat
      181 agagcatatc aaaagattca aggagaagca tccagagagc aaaatacttg ggcttaggat
      241 aaattaccga tctaagcaga gcataatcga tgtcttaaat tctgtaagct ctccgatgag
      301 gatgcctgag ataacgtgct gtgataagaa tgcggcttct gataaagatg tcattttgct
      361 tgagcatgac aacgaggaag cagagcatat gtttgtcgtg caaagcatcc ttaatctcag
      421 tatgcgaaaa gatgtatttg tccttgcaag gacaaacaag cagatagaga agatagcgga
      481 gaagctaaaa gcagcaggga tagactttgt caagagaacc atagaggaaa atggaggaga
      541 ggcaaaagag acacagatca cactgtcgac agtgcatgct ataaaaggcc ttgaggctga
      601 gatcgtctat ctcatctctg ctaacagctt cagttttcca tgcagggctt cagagcatcc
      661 tatccttgac atattaaaga ctcaaagtta tgataaatat gaggaagaat tacggctgct
      721 atatgttgca ttgtccaggg ctaagtcaaa gctgatcatc agttattact caagcctaag
      781 cccttttatc gatgataaaa ccaaaaaatt aattggaggg agagaagagg cgccaaagaa
      841 aggaaagccc agcctgtatg atgtccttaa aagatggcgc ctggaaaagt caagagagct
      901 aggtgttgct gcatttcaga tatttagcga caaaacaatc atggagatat gccaaaccgg
      961 tccagggaca atagaggagc taagggatgt ctatggcatg ggcccaaaca agatcatgag
     1021 atttgggaaa gatatagtca ggatggtaat agagaatgat tagctatcta gctttatatg
     1081 ctgcaagggc tcgtgcgatt atagaacggt cgatgcctgc aaagagtgaa tatgctatca
     1141 agcgaaaatc tgattcatca tcggcaaaat acagataatc tctttgacta actctttttg
     1201 ggcttggatt aacatcgtgg aaacttccaa gcgtcggctt taaattgttt tttctcctga
     1261 tatcaagcgc tgtttttcta aggctatatg gtgtctttag cgcaagttca agacctcttg
     1321 ggaagccatg atatgattca tgtaccttca tgttttttgg aagagcaagg ccatttccat
     1381 ggataatccg gcaattgtca agctcaatga aatcctctat tgtgccaccg aatgatcgat
     1441 atgcagccaa ccttatcctt gaatctttgt ctggatagct aagaggatat gcgccaaaga
     1501 gagacttatg ggtgtagaag ttcctttttg taccaaatgg atttatgcaa accgagaatt
     1561 ccttgccatc gcctgattca ccataaccta ctgatatcgg cctggcaagg ccaagtgcaa
     1621 tcttatcgtt gtatgctggt tgatacatct ttctggggaa cagatcaaga accaaacctt
     1681 tctgactgta tgggctgata agttagatct aaaaattttt agttataaat gatgtgttta
     1741 actagaattt ctggcttctt aacctttcga cccttttttc aacaggcgga tgggtcataa
     1801 aaaaggagag aaatgatctg cccctgaaag gatttgagat aaaaagatga gatgtcgcct
     1861 gggcttttgg tgttggtgca agagagatat gttttgccga ctgttcgagt tttagaagag
     1921 cgcttgcaag gccctcgccg cttccaacaa tccttgcgcc tgatgcatct gcaagatact
     1981 ccctacttct cgagatcgca agcctgataa tcgctgcaat aatcggagtg aggattgcaa
     2041 aaaccaagag ttctaatccg gagtttcctt cctcgtccct gccgtaccca aaaatagcac
     2101 tccatcttgc catcatcgca atataagaga tgacgccagc gatgactgcc gcaactgagg
     2161 agatcaatat atccctgttt tttatatgag aaacttcatg agcgataaca cctttgagct
     2221 cttcttcgtt taagagatca agaatccctc ttgtcgctgc aactgctgaa tgttttggat
     2281 ttcttcctgt ggcaaaagcg tttgcatatg gtgctgggat gacaaatact cttggcgtcg
     2341 gaatcttggc taaggaggat acttctttta cagccttata taatataagc tcttcttttt
     2401 ctgtgactgc ttttgcccga tacatggcca gggctatctt gtcgctgaac caataggcta
     2461 tgaagttaag gaggatagag aagattagcg ctatcaataa tcccgattgt ccccaaaagc
     2521 ttccaatcaa caataaaagg cctgtaagta tgccgaggag aagagcggtc ttcaactgat
     2581 ttcctatcgc catggtgtta tattaatggt tttttattta tatagttaat ggatttatcg
     2641 tcaaaatgat aaattagaga aagacaaaag ttagttacaa attggtttgt ctttctatat
     2701 ttagttgcgg cttttaatta taacctgtcc gcaactaaaa atccgcaatg tttataaaca
     2761 agttatttag aggtagtcta acaaaggcta actttagcca aaaataacta ttatgggggt
     2821 gtaaaataat ggctgcaaat acgacaaaga agcttggagt atgggctttt atatttggag
     2881 ttatcctagc cctgatcgtt ggactactag cagcaaatct tacagcatac atgggactga
     2941 tatctgccct gatggtattg ctgggattga tagtaggcat attaaatata actgaggaag
     3001 aagtaaacag cttcatcatc gctgcaatag gccttgcaac aggctcgatt gctattgcaa
     3061 accttggaac attacttcca ggcacagtcg gtgtgatgct gaagaacaca ttctcagtgt
     3121 tcagcgtttt tgttgctggc gcagtgttta ttcctgcact taagtcagta tacagaatct
     3181 caagggatta aacccttttt tattttttct ttctaggtaa ctatcaagac cttctatctg
     3241 cctaagctga gtatgcctaa acaagaaatg tctgaatcgg ttgtcaatcc ttacgccaaa
     3301 ttgatttagg tcatggttaa gctttgaata aagctgcttt ccttttttgt cgaggtctgt
     3361 caatattatg actggccttt ccttggcaac caattcttcg acgatcttga acagcgggcg
     3421 cctgttaagc tggattacac catctattcc gaggcgatgg agagctgctt tgtcttcctt
     3481 gccttctacg acgattatcc tttctccttc cctgagcctt tctatccagt caagcagcaa
     3541 ttcaatctct tccataacac ttaacgaaaa gcattataaa taaaaatatt ttcttagttg
     3601 gtatgataac cttaagtgaa acgcttaagc attacaaaca gccagacata caagaagaga
     3661 tcgtccttgc tgcaaaagac aaggaggtag ctatcaaatt tggagacaag ggttttggaa
     3721 aaaggcctga tgcactgaag tatcccaatg atgtccttga acttgcaaag caaggagcta
     3781 catcatttca ctcttctgaa gaattgtgga gcaatcctct gcagcttgat cccatgatga
     3841 agaaaaagga catggaagac ctgaggattg gatgggacct tgtcatcgat attgactgca
     3901 agtttctgga ttattcccag ataactgctg atctcctcat aaaggctttg aagtttcagg
     3961 ggataaattc tgtgtcgtgc aaattttcag gcggaagcgg gtttcatata ggggtgccgt
     4021 ttgaggcttt tcccaagaag gtgcatgaca gggaggcaag actttggttt cctgatggag
     4081 caagggctat tgcgctgtac ctgaaggaaa tgataagaaa acccctgtct gagaggatgc
     4141 ttgcttatga gaatcccgac aaaataatgg caaagactgg aaagccattt aatgagataa
     4201 tcaagaaagg gatatttgat gcttttgcgg tcgttggcat cgacaccatc cttatctcct
     4261 caaggcacct gtgcaggatg ccttattcat tcaatgaaaa aagcgggctt gtttcagtac
     4321 cgatagatcc agagaaggta atggaattta gaagagaaga tgctgcgtca acaaaggtta
     4381 gtgtctcgaa gtttaggttt cttgacagaa gctttgctat ggaaaatgag gcaaagaaac
     4441 tgcttgtgca ggcttttgac ttcaacctga agcttgagga agaaaagcca aaaaaagagt
     4501 atgaaatccc gcaggaagca atacctgaga aattttttcc tccttgcatc aataacatac
     4561 taaaaggggt ggatgacggc aagaaaagga gcatgtttat ccttacaaat tttttaaact
     4621 ctgtcggctg gagttatgaa cagatggaaa aatcacttga tgagtggaac aagaaaaaca
     4681 aagagcctct gcgggaagta ttattaaaag gacaactcag gtatcataag cagagggcaa
     4741 agaaaatcct tccgcctaat tgctcaaact ccatgtatta caaggatttt cgggtctgca
     4801 ctccagacaa tctatgccac aggataagaa atcctgtaaa ctatgcaagg gctaagacaa
     4861 gataccttga gaaagcaaag aaacctgaaa aggcacaaaa aagcaccaac acatagcaac
     4921 atttataaag ggtcccttac tcacaatatt aacaaaaaat agtgtaccta agtgattatg
     4981 tacatcatgc ctgaaaagga gccttttcag tacaacattt ctttaaaggc ttcaataaaa
     5041 ggcgattaat aaaacactca aaggaatatc tgtctttgag gtgagaacgc aaaacctaaa
     5101 aaatgccagc aacaagaaga ccgagatttg gaagtttggc tttctatcca aggaaaagag
     5161 ccaagagaga aactgcaaga gttagagcat gggctaaatc aaaagagcct aagctgcttg
     5221 gctttgcagg atataaggtg ggaatggccc atgttatggc cactgacaat tctccaaccg
     5281 cacttaccaa aggacagacg atctctatcc cctgtacaat catcgagtgc ccttcactga
     5341 aggcttattc tatcagattc tacaaaaata ccatttacgg ccaaaaagtt atttgtgatg
     5401 ttcttgctga aaatctaaat aaggagcttg caagaagaac catcatgcct aaaaatagga
     5461 agagcaaggt accagattca aaagattatg acgatgtaat gcttgcagtc tacagccagc
     5521 caggcttatc gggaatcggg aaaaagaaac ctgagatttt tgagataggc atcggcggcg
     5581 caaaaaaaga agacaaacta aagtacgctc aagaactcat tgcaaaggaa atatctgtaa
     5641 aagatgtttt tgctgaaggc aaccagcttg acatccatgc aatatcaaaa ggaaaaggcc
     5701 ttcagggacc tgttgcaagg tttggaatag gactaaagaa ccataaaaac gaaaagacaa
     5761 gaagaaaccc tggatcgctt ggaccgtggt gcggacaagt aaatatcatg tggaaagtac
     5821 cccatgcagg gcaaaccgga tatttccaaa ggatggacca taacaaatgg ctcctgaaga
     5881 ttggagcaaa aggagaggag ctttcaccaa agggaggctt tatgcattat ggattcatca
     5941 agaatcctta tataatagtc aaaggctctg tcacagggcc aaagaaaagg cttatcagat
     6001 tcaaccaggc aataaggcca aacaaaggac tgcctaaaga ggctcctgca ataaaacagg
     6061 tcgtattatg aaagaagcaa aagtagtgga tatcgaaaac aatgaaaaag gaaaggtaaa
     6121 gataccgagc cagttttcag aagagataag acctgaccta atcaaaagag cttatatcac
     6181 tttaatgtca catacaaggc agaggtatgg agcaacgcct atggcaggca agagagcttc
     6241 tgctattgtc tcaagacaga gacatgatta taggggagcg tacggccttg ggatatccag
     6301 agtcccaagg aagataatgt ccagaagagg cacaaggatg aactgggtcg gtgcgtttgc
     6361 gcctggaaca gtaggcggaa gaagagcaca cccgccaaaa gcagaaaaga tatggtggca
     6421 gaagataaac agaaaggaaa gaaaaaaagc tatcaggtct gctatggctg caacgcttga
     6481 caaggaaatc gtcagcaaaa gaggccatat catcggtgaa aactatccta ttatcgtaga
     6541 ttcatctttt gaagatatcc agaagacaaa gcaagttgag cagatgcttg ttaagatggg
     6601 atttgagaat gaacttagca ggtgctcaaa gaaaaagata agggctggtg tcggaaaaac
     6661 cagaggaaga aaatataaac aaaagaaggg ccctcttttt gtagtctcaa aatcatgcaa
     6721 attgataaag gcagctgcaa atatccctgg agtcgatgtt tgcgaagtca agaatgtcaa
     6781 cgtcaggctt cttgcgccag gcactgtgcc tggaaggctt acagtctgga gcaaggctgc
     6841 aatcgaaatc atagaaaagg agaaattatt tttataaaat ggaaaacgta ataaaaaatc
     6901 ctttgtctac agaaaaggca atacggctta tggagagcga gaacaagcta ttattcgtga
     6961 tagacagaaa agcgaccaaa aaggatgtta aggaagcgat agaaaacatg ttcaaagcaa
     7021 aggttgtcgc tgtgaataca ttagtggggc cagacggagt aaaaagagca tatgtgaaat
     7081 tttcagccga gacccctgct atcgatatcg ccacacaatt aggattgatg taaaaaatgg
     7141 gaaaaaatct aatccaacag aaaaggggaa agggctcatc tacttatcga gctccaagct
     7201 tcaagtatgt tggaagagca aaatatggaa catacggcaa cctaccatcc aaaggaacga
     7261 taactgacat catccattgt ccaggccata gcgcaccgtt gatatctgtc aagtatgata
     7321 atggcagcga tgtgctcctg caggcagcag aagggcttaa ggtcggaagc gaagttgcca
     7381 ctggcgacgg ggcttcttta atgccaggaa atgtccttgc acttaaagat atacctgaag
     7441 gtactgatgt atttaacata gaaagcatgc ctggagacgg cgggaagttt gtcagagctt
     7501 cagggaccat ggcaaaggtt atcgcaaaat caaacgacaa gataatactg atacttcctt
     7561 caaaaaaaca gagaaatttc agcccaatgt gcagggcgac aatcggaata atcgccggcg
     7621 caggaaagaa agaaaaacct ttcctcaagg ctggatttaa gatgcacgcg atgagggcaa
     7681 gaaacaagat atatccaaaa gtcgccggcc ttgcgatgaa cgctgtcgac catccattcg
     7741 gaggatcaag caccaatgca aaaggaaaat gttcaactgt atcgagagat gcacctccgg
     7801 gaagaaaggt cggaaagata gcggcaagaa gaacaggaag gaagagaagg taaaatggca
     7861 agaaaagaat tcagctacag gggaaaatca gtaagcgagc taaagagctt gagcttaaat
     7921 gaatatgcac agatagcacc tgctaggatc aggagaagca taaaaagagg atttaccgaa
     7981 cagcaaaaaa tactgcttgc aaatattagg gccaaaaaaa gcaacataga aacccactgc
     8041 aaggacatga taatacttcc tgagatgata ggaatgatga tcaagatcca taacggcaag
     8101 gaatttttgc ctgtaacgat agggaacgag atgatagggc attatcttgg cgagtttgtc
     8161 agcacaagaa aaagggtaga acactcagca ccaggtatcg gagctacaaa gagctcagca
     8221 gcactttcag taaagtaaaa tggcaaaata ctcaacagtg gactatgaaa aggaaaatat
     8281 ggcaagagct atcggaaggt ctctttccat cagcacaaag gccagcgtcg agatatgcag
     8341 ctttgtgaga gacaggtcca caacagaggc aaagaaactg ctaagcgagg tcatagagct
     8401 cgaaagagca gtaccctaca gggtgtacaa caaaggcctt gcccataaaa gaaaaatagg
     8461 cccgggaaaa tacccggcaa aggctgccgg cgaggtcatc agagtaatcg aaagcgcaga
     8521 ggctaacgct caattcaaag gacttaacac tgcaaaccta atcatcaaac acataaacgc
     8581 ccatcttgcc gcaaagcaat ggcactacgg caggaaaaga aggagaaggg ccaaaaggac
     8641 acatatagag atcatcgtcc aagaaaagaa acctgaaaaa gcaaaagaga aaaaggctaa
     8701 aaaatgatcg agcgaaaatt tgtaaacgag aaaataaaag aattccagat acaggagtat
     8761 gtcagaagca gccttaggaa cgcgggcctc agccatataa aggtgcagag aacccctctt
     8821 ggcgagaaga taatagtata tacatcaaga ccaggccttg tcgtcggaag aaaaggccag
     8881 aatatcaagg tgcttacaaa aacgctcaag aaagagtttt cacttgaaaa tcctcagatt
     8941 gaaatagcag aagtcggcaa catag
//
LOCUS       DASRUK010000053         6765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_384191, whole genome shotgun sequence.
ACCESSION   DASRUK010000053 DASRUK010000000
VERSION     DASRUK010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384191"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1328
                     /locus_tag="VFF28_02515"
     CDS             150..1328
                     /locus_tag="VFF28_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44534.1"
                     /translation="MKKTLIAALCLILLIVSVSAVERTLFLDDYQIKYSYEDAEEGER
                     FTLSVEMTNNGNNKTEVVLQLDDTGPFEIQGDDEWEIGTLEKNQKVSKTFRVEVDEET
                     PEDEYDLDFTLEDSRKEYEDEFEIATVFKDAELIIADVKSEPAIISAGQDDIKLTIKV
                     DNIGSADANSVVSRLNLPAGFSASSSYSNTANAGIIKAEGTSDLVFFIDTADNLKSGN
                     HQASLSLQYKAETTLETKNMEFDLPVKGIPRFELVSYNTIPAQTPVDSEGKLNIRIKN
                     IGEEEGEETSIRVFENADLPIEFDQKTNLIGSLRRGEEGTASFDLIIDEDAAPKGYII
                     KAQVRTVNKGNVLVDEFSVPITVVQPEKGSNYGLILGIALVILLAAIIYLIFLFRKRE
                     "
     gene            1331..1975
                     /locus_tag="VFF28_02520"
     CDS             1331..1975
                     /locus_tag="VFF28_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZX44535.1"
                     /translation="MLPKIIIHNSVSADGSLTGFEVNMGLHYDIAGNYKPDAHLIGSN
                     TAKTGIDTYGGRPETKSDLVKPSRDKELPYWAIIDTEGKLKGHLHNLRGFEYCKDVIV
                     FVSKATPQSYLDYLKERDYDHHVLGNLKVDLKKALVLLYDNYKVRKILTDTGRILSNL
                     LIEEKLADELSLLIHPVLVGEKSYKLFSVPGELRLVKSEVLEKDFVWSTYEMGK"
     gene            complement(2196..2357)
                     /locus_tag="VFF28_02525"
     CDS             complement(2196..2357)
                     /locus_tag="VFF28_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44536.1"
                     /translation="MKLWPKINCALNCPKDTPTKNCPECIRTIIGSQGAMSVDLCQME
                     GECHCAYEL"
     gene            complement(2465..4423)
                     /locus_tag="VFF28_02530"
     CDS             complement(2465..4423)
                     /locus_tag="VFF28_02530"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0043682 - P-type divalent copper
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HZX44537.1"
                     /translation="MANEDHSHHHELMVQDFKKRFFVSLALTFPVLILSPLIQQVLGF
                     SLEFPGDSYLLFALSAVIFFYGGLPFLKGIVSETKDRKPGMMTLIAVAITSAFVYSSI
                     VVFGLKGEVFFWELVTLIDIMLLGHWIEMRSVMGASRALEALAKLMPSEAHLMIGGKV
                     KDVPVSELVEGNIVLVKPGEKIPSDGIIIKGESSLNESMLTGESKPASRKKGEKVIGG
                     SINMEGSLEIKITHTGKDTYLSQVIGIVKQAQESKSKTQDLANRAATWLTIASVSIGA
                     LTLAVWLALGQGFAFSLERMVTIMIITCPHALGLAVPLVVAVSTAISAKNGFLIRNRV
                     AFENIRNIDTIVFDKTGTLTKGEFGVTDVITMKGYTEKEVISYAASLEAKSEHPIAKG
                     ITQKASEQKARTGKVEQFKALPGKGIQGLVNKKMIRVVSPGYLIEGKIKPDEQSKKLA
                     SQGKTVVYVLSQNTIIGVIALADIIRDESKEAIKNLQSMGIKCMMLTGDNKQVAEWVS
                     GELGLDDFYAEVLPDQKSKAIKRIQSKGMKVAMVGDGVNDAPALVQADAGIAIGAGTD
                     VAIESADIILVRNDPRDVASIIGLARKTYSKMKQNLFWATGYNIVAIPLAGGVLYSYG
                     ILLSPALGAAFMSLSTVIVAINARLLKI"
     gene            complement(4725..5006)
                     /locus_tag="VFF28_02535"
     CDS             complement(4725..5006)
                     /locus_tag="VFF28_02535"
                     /inference="COORDINATES: protein motif:HMM:NF039498.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5676 family membrane protein"
                     /protein_id="HZX44538.1"
                     /translation="MKKKDYSLEPKRFGLAVGSTLGLLYIICAAVILAIGREGVIFFA
                     NALFHGLDFSTLVKTSFSLPGFLIGLVSYIVLGWIMGAMIAYIYNLAKR"
     gene            complement(5012..5362)
                     /locus_tag="VFF28_02540"
     CDS             complement(5012..5362)
                     /locus_tag="VFF28_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44539.1"
                     /translation="MKKKEARQNRPNIILWIAIALIAGTLIGYSLGKQDQGFSRDYIQ
                     GTASMMQTNGGSMMQMARMMMDSGRMMKERGAAIGDNSMMEQGDDLLQNGQMMETRGS
                     EMMGRANGMMDMMR"
     gene            complement(5478..5705)
                     /locus_tag="VFF28_02545"
     CDS             complement(5478..5705)
                     /locus_tag="VFF28_02545"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HZX44540.1"
                     /translation="MMWQYGGYYGWGFMGLTMIAGMVLFWGAIIWLIVWLVNRDRKVI
                     RERPLDILQRRYARGEITRKEYEDMKKDLDR"
     gene            complement(5743..5907)
                     /locus_tag="VFF28_02550"
     CDS             complement(5743..5907)
                     /locus_tag="VFF28_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44541.1"
                     /translation="MKKHTLLMIGCIAVPLLLIILLPILGIKNNYIIPIAMLIMCGSH
                     LFMMGDHKHH"
     gene            complement(5981..6100)
                     /locus_tag="VFF28_02555"
     CDS             complement(5981..6100)
                     /locus_tag="VFF28_02555"
                     /inference="COORDINATES: protein motif:HMM:NF040898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LDCC motif putative metal-binding protein"
                     /protein_id="HZX44542.1"
                     /translation="MAFKKIKEKFSSWMKSLAAANEKEYGKKELSCCGMNKQK"
     gene            complement(6191..6616)
                     /locus_tag="VFF28_02560"
     CDS             complement(6191..6616)
                     /locus_tag="VFF28_02560"
                     /inference="COORDINATES: protein motif:HMM:NF021587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2318 domain-containing protein"
                     /protein_id="HZX44543.1"
                     /translation="MKRKTLAWTGIALLALLAIVVVNGGHKGNQADSTYVRIPLSELS
                     TTAKFYTYGNARYFAVLGSDGQPRTAFDACQTCGPKGYTQNGNNMKCKNCGLEFSIDS
                     LGVENTGYGCWPGYLPSEVAGGEILIKKSDLAGSKFFKQ"
BASE COUNT         1920 a         1521 c         1336 g         1988 t
ORIGIN      
        1 attgatgtat cttggcaatg ttgaccatgt aatcgaagta ttcaagttct ataactcacg
       61 cccaaagtaa tttgtgcgca aagccctttg aaagaaaaca cttaaaaagc aaaaacaaat
      121 cccaacaacc agcgtagagg gtgaaattta tgaaaaaaac actgattgca gcattatgct
      181 tgatattatt gattgtaagt gtatcagcgg tcgagagaac cttgtttctg gatgattacc
      241 aaataaaata cagctatgag gatgctgaag agggagaaag gtttacttta tctgtcgaga
      301 tgaccaataa tggcaataac aagacagagg ttgtcttgca gcttgatgat acagggccat
      361 ttgagataca aggggatgat gagtgggaga taggaacact tgaaaaaaac cagaaggtgt
      421 caaagacatt cagggtagag gtggacgaag agacgcctga agatgagtat gatcttgatt
      481 ttacgcttga agacagcagg aaagaatatg aagacgaatt tgagattgca actgttttta
      541 aggatgcaga gctgatcatc gctgatgtca aaagcgagcc agctatcata tcagccggcc
      601 aagacgatat caaacttacc attaaggttg ataatatcgg cagtgctgat gcaaactctg
      661 ttgtttccag attgaacctg ccagcgggtt tttctgcttc aagttcttac tcaaatactg
      721 caaatgcagg gataatcaag gcagagggca catctgactt ggttttcttc atagacactg
      781 cggacaatct caaatcaggc aaccatcagg caagtctaag cttgcagtat aaggcagaga
      841 cgactttgga gacaaagaac atggagtttg acctgcctgt aaaaggaatt ccaaggtttg
      901 agttagtttc ttacaacact attcctgccc agacacctgt cgattctgaa ggaaagctca
      961 acataaggat aaaaaatatt ggcgaagaag aaggagagga aacttcgata agggtttttg
     1021 aaaacgctga tcttcctata gagtttgatc aaaagacaaa tcttataggc agccttagaa
     1081 gaggagagga aggcacagca agttttgatc taataataga tgaagatgca gctcctaagg
     1141 gatacataat caaggctcaa gtaaggacag taaacaaagg caatgtgctt gtcgatgaat
     1201 tcagtgttcc tataactgtt gtgcaaccag aaaaaggatc taattatggg ctgatacttg
     1261 gaattgcgct tgttatatta ttggcagcta tcatatatct tatattcctg tttaggaaaa
     1321 gagaataaat atgctgccca aaataatcat ccataactcg gtaagcgctg atggttcttt
     1381 gacaggcttt gaagtcaaca tggggctgca ttatgatata gctggaaact ataaaccaga
     1441 tgctcatctt atcggctcaa acactgcaaa gacagggatc gatacgtatg gcggaaggcc
     1501 tgaaactaaa tctgatcttg tgaagccaag cagagacaag gagctgcctt actgggcgat
     1561 aatagataca gaaggaaagc tcaaagggca tcttcataac ctgcgcgggt ttgaatactg
     1621 caaggatgtg atagtgtttg tttccaaggc cacccctcaa tcctatcttg actaccttaa
     1681 ggagagagat tatgaccatc atgtccttgg aaacctgaaa gtcgacctta agaaagcgct
     1741 tgttcttctt tacgacaatt acaaagtaag aaaaatactc acagacaccg gaaggatact
     1801 ttcaaacttg ctgatagaag aaaagcttgc agacgagctt agcctgctta ttcatcctgt
     1861 gctggtggga gaaaaatcat ataagttgtt tagcgttcca ggtgagctaa ggctggtgaa
     1921 gagcgaagtc ttagagaaag attttgtttg gtccacgtat gaaatgggaa aataggctgc
     1981 ttttggcagc ctttggcatc acctccacca aatgttttct ggcaaagaga tatagggtaa
     2041 gcgtttatta atgccttatt aaagataaat ctgatttgtc ttccataaga gcactcataa
     2101 aaaaacctcc gcatttacta tcgaaagaaa taaggaagaa aaggatgcct agcagcaccc
     2161 tttcttttta tttttgtcag gcatttattc acctcctata attcataggc acaatggcat
     2221 tcaccttcca tctggcataa gtcaacgctc atcgctcctt gagaaccgat aattgtcctt
     2281 atgcattccg gacaattttt cgtgggggtg tccttggggc aattgagtgc acagttgatt
     2341 ttcggccata gtttcaaatt ttctcacctc cggcaagatc tattagtgca ttcttattta
     2401 tatacgatgg gtaagaataa tctgaggtga cttacataaa ctttaaaaat aaaaaattag
     2461 aaaactagat cttaagcagc cttgcattta ttgcgacaat aactgtgcta agcgacataa
     2521 aagctgctcc aagagcaggg cttaacaaaa tgccatagct gtataacact cctcctgcaa
     2581 gaggtatggc aacaatatta tatccagtcg cccagaaaag gttctgcttc atcttggagt
     2641 aggttttcct tgcaagccct attatcgaag caacatctct tggatcgttc ctgaccaaga
     2701 taatatctgc cgactcaatt gcaacgtctg tcccagcacc aatcgcaatc cctgcatctg
     2761 cctgcactag ggcaggagca tcattaacac cgtctccaac catggcaacc ttcatacctt
     2821 ttgactgtat ccttttgatg gctttggatt tttgatcagg caggacctca gcatagaaat
     2881 catcaaggcc aagctcacca gatacccatt cagccacttg tttgttgtct cctgtaagca
     2941 tcatgcattt tattcccatc gattgaaggt ttttaatagc ttccttggac tcatccctta
     3001 ttatatctgc aagagctatc acccctataa ttgtattttg tgaaagcaca taaacgacgg
     3061 ttttgccttg agaagcaagc tttttgctct gctcatcggg ctttatctta ccctctatca
     3121 gataacctgg gctcacaacc ctgatcattt ttttatttac taatccctgg atgcctttgc
     3181 ctggcagcgc tttgaattgc tctacttttc ctgtccttgc tttttgttcg ctggccttct
     3241 gggttatgcc ttttgcgatg ggatgctctg atttggcctc aagggaagct gcataggaga
     3301 taacctcttt ttctgtatat cctttcattg tgatcacatc ggtcactcca aattcacctt
     3361 tggtcaaggt acctgtttta tcaaatacga tggtatcgat gtttcttatg ttctcaaagg
     3421 caaccctgtt tcgtataaga aatccatttt ttgcagatat tgcagttgat accgctacaa
     3481 caagcgggac tgcaagccct agtgcatgag ggcaggtgat tatcattatt gtgaccatgc
     3541 gttcaagtga gaaagcaaaa ccttgcccaa gcgcgagcca aacggcaaga gtcaatgcac
     3601 ctatcgaaac cgaggctatg gtaagccatg ttgctgcgcg gttggccaga tcctgagtct
     3661 ttgatttgga ttcctgggct tgtttgacaa tgccaataac ttgggacaaa taagtgtctt
     3721 ttcctgtatg ggtgatcttt atttccaggc ttccttccat atttatcgag ccgcctataa
     3781 ctttctcccc tttttttctg cttgcaggct ttgactctcc agtaagcatc gattcattta
     3841 ggctgctctc accttttatt ataattccgt ctgaagggat tttctctcca ggcttgacca
     3901 aaacaatatt tccttctaca agctcagaga caggaacatc ttttactttg ccgcctatca
     3961 tcaagtgtgc ctcagaaggc ataagctttg ccagggcctc gagtgctctt gatgcaccca
     4021 tcacagagcg catctctatc caatggccaa ggagcataat atctatcaga gtaacaagct
     4081 cccaaaagaa tacctcacct ttaagcccaa atacgacaat actgctgtat acgaacgctg
     4141 aagttatcgc tacagcgata agagtcatca tcccgggctt tctatccttt gtttcactca
     4201 ctataccttt cagaaaagga aggccgccat agaagaatat aactgcagac aaagcaaaaa
     4261 gcagatagga gtcacctggg aactcaaggc taaatccaag aacttgttgg ataagcggtg
     4321 agagtataag gacaggaaat gtcagcgcaa gagacacaaa aaatctcttc ttgaagtcct
     4381 gcaccatcaa ctcatggtga tggctatggt cctcatttgc catcttctga tggctgggag
     4441 caggggactc ttcatccccg actttcacaa gcttagtgcc gtccatagtg cactttcctg
     4501 gcttgtcata ttccatcccg cattttgggc atttgtattt tgccatcaag acaacctcca
     4561 atcaaactcc ctttctgcac tatctttgat taccaacctt ataaacttag cattttcctg
     4621 tcggtggaaa tattaggtac gcgatgataa aataaaacaa aaaaagagat atagttgcca
     4681 agaatctggt tattatatga ttctttttct ttcctgccat atacctatct tttagctaag
     4741 ttatagatat aagcaatcat ggctcccatt atccatccaa gcacgatgta actgacaagt
     4801 cctatcaaaa agccaggcaa agaaaagctt gtcttgacaa gggttgaaaa atccagaccg
     4861 tgaaataatg cgtttgcaaa aaatattact ccttcacgcc caatagcaag tatcacagcg
     4921 gcacaaatga tatatagtaa acccaaggta gatcctacag caagcccaaa tcttttgggt
     4981 tcaagcgaat aatctttctt tttcatagct ttcacctcat catatccatc atcccgtttg
     5041 ctcttcccat catctcactc cctctggttt ccatcatctg accgttctgc agaagatcgt
     5101 caccttgctc catcatgctg ttatcgccta ttgcagctcc cctttctttc atcatccttc
     5161 ctgaatccat catcatcctt gccatctgca tcatggagcc gccgttggtc tgcatcatag
     5221 aggcagtgcc ttgtatatag tcccttgaga aaccttggtc ttgctttccc aggctgtatc
     5281 ctataagtgt tcccgcaatc aatgcaatcg caatccacag aatgatattt ggcctatttt
     5341 gtctagcctc ttttttcttc atcagtcacc tctttggcag gcaagcgctt gccatagcga
     5401 tttagaactg atagctattt aaagattgcg ttaaaagtta acaatagtta acttgtaagg
     5461 ttagttatcc gcacgagtca tctgtccaga tcttttttca tatcttcata ctcttttctt
     5521 gttatctctc ctcttgcata ccttctttga agaatatcaa gcggccgctc tcttattacc
     5581 tttctgtctc tattgaccag ccagactatc aaccagatga tcgctcccca aaacaagacc
     5641 atacctgcta tcatcgtcaa acccataaat ccccacccat aatatccccc atattgccac
     5701 atcattttct atcaccccgt ctacaataag actttgttgt atttaatggt gtttgtgatc
     5761 tcccatcata aacaagtgtg aaccgcacat tatcagcata gctatgggga tgatataatt
     5821 attctttatc ccgagaatag gcagtaatat tataaggagc aaaggaactg caatgcatcc
     5881 tatcatcaat aaagtgtgtt ttttcatctt taacctcaaa aaagtaaaag acaccaggca
     5941 ggggggttag aagtgccttt ctggtgcttt actaataaag ttatttctgt ttattcatgc
     6001 cgcagcagga tagttctttt tttccgtact ctttctcgtt tgctgctgcc agcgacttca
     6061 tccagctgct gaatttttcc tttatttttt taaatgccat gaatcctctt tttgcctcaa
     6121 cctgccgccg ggctgaaaaa gcctcggggc gtgttcttga gcccaattca ctccaccgac
     6181 tggtggaatt tcattgttta aaaaatttac ttcctgcgag gtcgctcttt tttattaata
     6241 tctctccgcc agcaacctcg cttggcagat accctggcca gcagccatag cctgtgtttt
     6301 ctactccaag actgtcaatg gaaaattcaa ggccgcaatt tttgcacttc atattgttgc
     6361 cgttctgagt gtaccctttt ggaccgcagg tttggcaggc gtcaaatgca gttcttggct
     6421 gtccgtcgct tcccaggact gcaaagtatc tggcattgcc ataggtataa aacttagcgg
     6481 tcgtgcttag ctcagacaat ggaatacgga catatgtact gtcagcttga tttcccttat
     6541 ggcctccatt taccaccact attgcaagaa gcgcaagcag ggcgatgcct gtccaggcca
     6601 atgttttcct tttcatgaaa acaaaccagc tcctgcttcc aaatcactga cttttataat
     6661 gacgtcgctt cctgataccc catactcaag atatgaaggg aagcatccct ctttggcatt
     6721 gccagtgcct atatcatcga tgggaaagct ttgtccgcac gcttt
//
LOCUS       DASRUK010000054         6112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_710423, whole genome shotgun sequence.
ACCESSION   DASRUK010000054 DASRUK010000000
VERSION     DASRUK010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 6112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6112
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710423"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..380)
                     /locus_tag="VFF28_02565"
     CDS             complement(147..380)
                     /locus_tag="VFF28_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44544.1"
                     /translation="MHTGIITLSEKYITKEMIQYAYNQLDSAKIFTSKGLADNFPHEY
                     SQGACVYSMTGGILELLGIAEYKKRKGYTKRRN"
     gene            complement(573..899)
                     /locus_tag="VFF28_02570"
     CDS             complement(573..899)
                     /locus_tag="VFF28_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44545.1"
                     /translation="MYEKKSIIEFITQAGNLLHNEVNVYLIGGCNLSIKGLKDATKDV
                     DMVLDNHASFRVLKAALEKIGFKVDSSMLQEQVYRDAVIVFFDDSGSRIDIFVNKICS
                     MLQLTP"
     gene            complement(874..1812)
                     /locus_tag="VFF28_02575"
     CDS             complement(874..1812)
                     /locus_tag="VFF28_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44546.1"
                     /translation="MKMSKSMVKILVCLARNIESLDGLKKETGLSINRCSEVGTELEK
                     MGLAKKDRSRKGLSIRLADSVVADNFRKMYLSIPYMPYDKFLYGKKLEVLKTILYEEK
                     GVHTIAKMTGLKERTVRENLRALSNPPLLWKAGRKYILSKKGYPLIYNFLDSLRKYTA
                     ENKMILWKFNDEEVFKVWDIKLIKGSITGLNAFAGFGVEVMTVEYCCHVPERKLSKNE
                     IFIHGLLEVNEPRLLGIAIAFYLKNSLEKEDLGFLLSKYDLSGAFIELKKTINEFTYN
                     GKETIANEKIPSITPREIGRILDIYGVKYVRKEKHN"
     gene            2081..4693
                     /locus_tag="VFF28_02580"
     CDS             2081..4693
                     /locus_tag="VFF28_02580"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HZX44547.1"
                     /translation="MTHHHLGIQESLDSLSSSIEGISDEEAGKRIEKYGHNELVEQAK
                     RGPIKIFFDQLTATMQLILLGAALISVFLGSYKDAIAIFSIVILFAILGFIQEYRAEK
                     SMAALKKLAVPFVKVIRHGKLKEIPSINLVPGDIVSLEAGNIIPADCRLIESVALKVQ
                     EAALTGESVPVEKDAKAVCKEDDALADRITCVYMGTIISQGRAKAVVTSTGMNTELGK
                     IASVLQEVKQEATPLQKRLDSVGKKVALLGIAISIIIIIFGLIRGDSFTFLLLSGISV
                     AVAVIPEGLPAVVTITLAIGAQRMLKRKALIRKLPAVETLGSVTVICSDKTGTLTENK
                     MAVVNIDTMDEKVDLTRSKPQKSSLIMSALALCNDAKLDGKGFIGDPTEGALLIAAAD
                     MNIYKEEYEKCLPRVLEVPFDSERKRMSTLHEINDKDLLKSIGLEPKTRYISLMKGAV
                     DGIVHIADKVFIAGKEYRLTDELRKDLLDSNERLAGEGKRVLGSAYKFVDTEDVSDVE
                     KEVVFIGMVAIIDPPRQEVKESVEKCKQAGIRTVMITGDHPLTAKSIASQLGILGSGK
                     VITGKELDNLSEDGLKELVGKVSVYARVSPEHKLRIIQALQMSGQVVAMTGDGVNDAP
                     ALKKADIGVAMGITGTDVSKEASDMVLLDDNFKTIVSAAEEGRIVFDNIKKFVKFSLA
                     GNIGKIIVMLFGPVIWASLPLFPIQLLWLNLLTDGLLGLGLGMEKAESHIMKRPPIKP
                     KEGVFSNGGWMQVTVVGILVGVISIGIGAFYFFKGSDAWQTMIFLTIAFCQVWQVLAV
                     RTERDLSIGKSLFGNKTLISMVSVVILLQIAALYIPYLQSFLQLKSIGFIDLSVAFFS
                     GIVLFIVVEIMKKK"
     gene            complement(4703..4903)
                     /locus_tag="VFF28_02585"
     CDS             complement(4703..4903)
                     /locus_tag="VFF28_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44548.1"
                     /translation="MSEIAIGAHAGVLTWSKPAISECIEMQSISNKPEICRANFSVHA
                     QEHAPGFSPAVLDIPGLGYMVP"
     gene            4984..5274
                     /locus_tag="VFF28_02590"
     CDS             4984..5274
                     /locus_tag="VFF28_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44549.1"
                     /translation="MFLKTEIWYNDTLRRRCILNDRYSKDLGVILDTHVFGLSLALHE
                     VPNDESWKAPKEYPFFLGSYRLGIEQDNGCPISAAAYPESYASLFAVFPHDQ"
     gene            complement(5729..5941)
                     /locus_tag="VFF28_02595"
     CDS             complement(5729..5941)
                     /locus_tag="VFF28_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44550.1"
                     /translation="MGQQEVYNILKKYRRKWLSSRDIVKLLRSSPGSVITSLQKLRKG
                     DVINFKLERQKGQSVKRRVYVYKYKK"
BASE COUNT         1794 a         1229 c         1250 g         1839 t
ORIGIN      
        1 accttgtttt ctttgttata cagtctctta aaaatacttg acgaatgatg aaatttggtt
       61 cttaatgcca tctatatcac ctcttgctaa ttacagaagg ggtacttata taagaacttt
      121 tctatttaac ttactataaa tcaaagctag tttcttcttt ttgtgtatcc cttacgtttt
      181 ttatattctg caatacccaa taactcaaga atacccccag tcatagaata aacacaggct
      241 ccttgactat attcatgtgg aaaattatcg gctaaacctt ttgaggtaaa aatcttagca
      301 ctatcaagtt gattatatgc gtattgaatc atctctttag taatatattt ttcagacagg
      361 gttattatgc cggtgtgcat atatatctga gaaccaatta gtctagttac tggtcgggtt
      421 ctaccacctg tcttcgggaa ctgggttccg acacatactt ctttatctat ttgtttagat
      481 atttctttcc aactaatatt tattcgccct caaaggacta agaaggtatt ctttattaat
      541 gttgacttaa taactaacta ctatggcaaa tattatgggg taagctgaag catgctgcat
      601 atcttgttca caaatatgtc aatccttgag ccgctgtcat caaagaatac aatcactgcg
      661 tccctgtaaa cctgctcttg cagcattgag ctgtccacct taaagcctat cttttccaaa
      721 gccgccttaa gcaccctgaa gctggcatgg ttatcaagga ccatatcaac atcctttgta
      781 gcgtccttca atcctttaat gcttaggttg cagccaccga taagatagac attaacctca
      841 ttatgcaaaa gattgcccgc ctgtgtgata aactcaatta tgcttttctt ttcgtacata
      901 ttttacacca tatatatcta aaattctccc aatttctctg ggagtaattg agggaatttt
      961 ctcgttagct attgtttctt tgccattata tgtaaattca ttgatggttt tcttcaattc
     1021 aatgaatgct cccgaaagat catactttga aagcagaaag ccaaggtctt ctttttcaag
     1081 gctgtttttc agatagaaag cgatagctat tccaagaagc ctgggttcgt taacttcaag
     1141 aaggccgtgg ataaaaattt cattcttaga cagctttctc tcagggacat ggcagcaata
     1201 ttcaactgtc attacctcta ctccaaagcc tgcaaaggca ttcaagccag tgatactgcc
     1261 tttaatcagc ttgatatccc agactttaaa cacttcctca tcgttgaatt tccaaagtat
     1321 cattttattt tccgcagtat acttccttag cgagtccaga aaattataaa tcaatggata
     1381 gccttttttg ctaaggatat actttctgcc tgctttccaa aggagaggag ggttgcttaa
     1441 agccctcaga ttctccctca ccgtcctttc ctttaggcca gtcatctttg cgattgtatg
     1501 cactcctttt tcctcgtaaa gaattgtttt tagcacttca agctttttgc catagagaaa
     1561 tttatcatat ggcatatacg gaatagaaag atacatcttc ctgaaattgt ctgcaactac
     1621 agaatcagca agccggatag atagtccttt tctgctccta tccttttttg ccagccccat
     1681 tttttcaagc tcagtcccta cttcagagca tcggtttatg gataagcctg tctctttttt
     1741 tagtccgtca aggctttcta tattcctagc caggcatacg agaatcttga ccattgattt
     1801 tgacattttc atataatata catataattc gattttattt ataaatttat cgaaaaaaac
     1861 gtatatttaa ctaaatgtgc ataaaaagcg aaactgtaat gtaaaggagt tgctaaaaat
     1921 aggtatattt tgctaatcaa tcccccctat accaaataat cgtattttct tatttctccc
     1981 tggacattct ttcttgagca tgttccagct accaaagagg gggccatgaa aatgaataaa
     2041 taataaccat tttaaacccc cgtcttcttc tctgctgtcg atgacgcatc accatctagg
     2101 tattcaagaa agcttggatt cattatcctc ttctatagag ggcatttcag acgaagaggc
     2161 aggcaaaagg atagaaaaat acggccataa cgagcttgtg gaacaggcca aaagagggcc
     2221 aataaagata ttcttcgacc aactgacagc taccatgcag cttatactgc ttggagctgc
     2281 gctcatctca gtatttttgg gatcatataa agatgccata gcgatattct ctattgttat
     2341 cttgtttgcc atacttggat ttatccagga gtatagggct gaaaaatcta tggcagcctt
     2401 aaagaaactt gccgtgccct ttgtaaaggt gataaggcat ggaaaattaa aagagatccc
     2461 ttcgattaat ctagtgcccg gagatatagt ctctcttgag gcaggaaaca taatccctgc
     2521 tgactgcagg ctcatagaat cggttgcctt gaaagtgcag gaggcagccc tgacaggaga
     2581 atctgtcccg gtcgagaagg acgcaaaggc agtatgcaaa gaggatgacg cccttgcaga
     2641 caggataact tgtgtctata tgggaacgat aatcagccag ggaagggcaa aggctgttgt
     2701 gacgtctaca gggatgaata cagagctggg aaagattgca tcagtcctgc aggaagtaaa
     2761 acaggaagca actcctcttc aaaaaaggct ggatagtgtt ggaaaaaagg ttgcactgct
     2821 aggtatcgct atctcgatca taattataat atttggcctt attcgagggg attcattcac
     2881 ttttctatta ttatcaggaa taagcgtggc agtggcagtg attcctgagg gtttgcctgc
     2941 tgttgttacg ataactcttg cgattggcgc gcaaaggatg cttaaaagaa aagctttgat
     3001 aaggaagctt ccagctgttg agacccttgg ctctgtcaca gtgatatgct ctgataagac
     3061 aggcacgctt acggaaaata agatggcagt tgtcaacata gacaccatgg atgaaaaggt
     3121 tgacctgact aggagcaaac cgcaaaagtc ctcgcttatc atgtcagctt tggccctgtg
     3181 caacgacgca aaattggatg gcaaaggatt catcggcgat ccgacagaag gagcgctttt
     3241 gatcgctgcc gcagatatga acatatacaa ggaagagtat gaaaaatgtc ttccaagggt
     3301 tttagaggtc ccttttgatt cagagagaaa acggatgtct actctccatg agataaatga
     3361 caaagaccta ttgaaaagca ttggacttga accaaaaaca cgttatattt ccttgatgaa
     3421 aggcgctgtg gatggtattg tccacatagc agataaagtg ttcatcgcag gcaaagagta
     3481 tagactgacg gatgaactca gaaaggatct gttagattca aatgaaaggc ttgcagggga
     3541 gggtaaacgt gtcttagggt ctgcctacaa atttgtggat actgaagatg tgtctgatgt
     3601 tgaaaaagag gttgttttca taggtatggt cgccattata gaccctccaa ggcaagaagt
     3661 aaaggaatca gttgagaaat gcaagcaggc aggaataagg acagtcatga taacaggaga
     3721 tcatcctttg acagcaaaat ccatagcttc acagttgggg atcctgggta gcggcaaggt
     3781 aatcacagga aaagagttag ataacttatc tgaggatggg ctaaaagagc tggttggcaa
     3841 agtttctgtc tatgcaagag tatctccaga gcataagcta aggataatac aagcattgca
     3901 aatgagcggt caagtggtgg cgatgaccgg cgatggtgta aatgacgctc cagcactaaa
     3961 gaaagcagat attggagtag ccatgggaat aactggcact gacgtcagta aagaagcctc
     4021 agatatggta cttcttgacg acaatttcaa gacaatagtt agtgcagcag aagaaggaag
     4081 gatagttttt gacaatatca agaaattcgt aaagttctct cttgcaggta acattggaaa
     4141 gataatagtc atgctttttg gtcctgtcat ctgggctagc cttccgttgt ttccaataca
     4201 gctattatgg cttaatctcc tgacagacgg ccttctaggt cttggcctgg gcatggaaaa
     4261 ggcagaaagc cacatcatga aaaggcctcc gataaaacca aaagaaggcg tttttagcaa
     4321 tggaggctgg atgcaagtta ctgttgtggg catattggta ggtgtaatat cgataggtat
     4381 tggtgcattt tacttcttca aaggcagtga tgcttggcag accatgattt tcctgactat
     4441 agctttctgc caggtctggc aggtgcttgc agtaaggaca gaaagagact tgtctatcgg
     4501 gaaaagctta tttggcaata aaaccttgat ttcaatggta agcgtcgtga tccttctaca
     4561 gatagcagcg ctgtatatcc cctacttgca gtcattcctt caattgaaaa gcataggctt
     4621 tatcgactta tcagtagcct tttttagcgg catagtcctg ttcatagttg ttgaaataat
     4681 gaagaaaaaa taggtattca aattatggca ccatatatcc tagcccagga atgtcaagaa
     4741 ccgccgggct gaagcccggg gcgtgttctt gagcatgaac tgagaaattt gctctgcaaa
     4801 tttctggttt gttagaaatg ctttgcattt ctatacactc agaaatcgct ggcttactcc
     4861 aggttaaaac gccagcgtgt gcgccaatag cgatttctga cataccaaaa actcgaagcg
     4921 gccccctttt caaactattt ggtccgcaag taaatcttaa tctatggata ctagcaggaa
     4981 ccaatgttcc tgaaaactga aatttggtac aatgatacat taaggaggag atgtatctta
     5041 aacgacagat atagtaaaga tctaggcgtc attctagata ctcatgtttt tggtttaagc
     5101 ttagcgcttc atgaagttcc aaacgatgaa tcctggaaag ccccaaaaga atatcccttc
     5161 tttcttggaa gctaccggtt aggtattgag caggataacg ggtgtccaat aagtgctgca
     5221 gcttaccctg aaagttatgc atctttgttt gctgtctttc cccatgacca ataaaaaatc
     5281 ccaaccgcag caagctgcgg agtatttttt aagcctcaaa ctggattatt aagccgtagc
     5341 aagctacggg gtattaaccc agtttgagca atcaagcgat gcaggagtga tattatcttt
     5401 aaatgaagag gctcgacgga aagttccaaa agataagagg cgtgctgtct taagggcaac
     5461 ttgtcagctt gcttcattat tgcccgaaga attaggttca actccagaga attctatctc
     5521 gataatttgt taactcagat gcaaagagaa ggattcctat tcttcttctc gaccaagagg
     5581 tatcttatct atttccttgc atttatcctc agcgcacact tcatccttaa gaaaaaaatc
     5641 atgctctttt ttggatttct ttggtttttc tgccattggt actgcaaagc agattaagaa
     5701 tttaaattta tgcttggctg gttagaaatc acttcttata cttatacaca taaactcttc
     5761 tcttcacact ttgccctttc tgcctttcaa gcttgaaatt tatcacgtcg cctttgcgaa
     5821 gcttctgtaa agaggttatc acagaaccag gagaagatct aagcagcttc actatatccc
     5881 ttgaggaaag ccactttctt ctatactttt ttagtatgtt gtatacttcc tgctgaccca
     5941 tatgctactg ataagggcaa ccctttttaa tactttcgcc tattcggcac tgttaactct
     6001 tcggcataca tgaaatctct atcaatttca gcagcttatt tcttcccaag tcaagtttga
     6061 tgcctgcagc ctcggcaggc gtcctgcctt taagttccat atttggattg ac
//
LOCUS       DASRUK010000055        14781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1557805, whole genome shotgun sequence.
ACCESSION   DASRUK010000055 DASRUK010000000
VERSION     DASRUK010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 14781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14781
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557805"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VFF28_02600"
     CDS             complement(<1..547)
                     /locus_tag="VFF28_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44551.1"
                     /translation="MVTMIASRHQETEFNRGSHIGTIVLQSVDDEPLNMNGLIAAYMS
                     AIILGRIRADEIDSTPLVRGYDGARFDAIRQAANSGVWTPVTVDPRIVEVDFAGHSSV
                     PLEETKFRNPGEHMYYCFCPPSPHHRVPLTKNPVVEAEKAELLAGLRRIYDGIEVAVK
                     SANGYLLTSLPELIVPYSPRMK"
     gene            677..832
                     /locus_tag="VFF28_02605"
     CDS             677..832
                     /locus_tag="VFF28_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44552.1"
                     /translation="MKKKIWETSTRDELQNAGARFNSFGNDEDFYAILNGGQIFVKRF
                     GDSYRQI"
     gene            972..1118
                     /locus_tag="VFF28_02610"
     CDS             972..1118
                     /locus_tag="VFF28_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44553.1"
                     /translation="MEYGETDDESIYNEEVRDELLDRDALAASEVGFMIGYDEAYDAE
                     DGVV"
     gene            1317..3893
                     /locus_tag="VFF28_02615"
     CDS             1317..3893
                     /locus_tag="VFF28_02615"
                     /inference="COORDINATES: protein motif:HMM:NF016980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44554.1"
                     /translation="MNQSRTILAMLVIFLFGSANISFAVDLGDFPAPFIQGNEMRGYL
                     VVGDRASAEDIVGVSDIAVSLQYAISGGSGSGRSNFSVSGATWQVGTTSSQLEMAQNT
                     DTFSGETIGDIRTTIDETELSLLTDGSITTEKGTSRYNQYLIFPSDNQSSYVIFAEND
                     NDTASDFLYFRSGQGIGRYSLEFATSLQSDVEDSAGARTATGTYLGDYEDEIITIMGK
                     EFNIVTARRTSATTNSIELTLMGGAVRDTLEEGETKTYTVEGKEYEVTATAITDTAPI
                     YARFNINGESVRSLSDGETATLSDGTEIGVVDIVPNEAGDPTQDLVEFFVGASKLFLK
                     DSDVTNTASSNALEVGTERVDDSAVIITGSNDNATFSIDTIQVNITADDDFYVGAGQK
                     LSQFMDEPEALLGVWDIQYGGLASLGTETYRLRSSGSDQYDLVFVDGDGDSANLPLVY
                     SNVSSQIKLGDRDDDLIVNGSLVISKNDYFVISYGGQQAGDRKTYAFRYRGADKVAAG
                     ETAQVRLQNLGTGDTSENTFRDNTGDTADATLKVGGSTFNIINASAATSNDFDIMVDL
                     DGDGTTAGDTDIIPIDTNFGQTITFTVGTPTYLVVAFTTPNTNDYDNIAPAAVTMNIT
                     AASDREVSFAESSGSTINWISPTEEDNINYAYTSMGARYRWSNPSNDPDDLEVSYPRV
                     QRLPLVYISGSNATTATVTSGETTTAGNRIEVGAVRLASEVQTPSAQNLIIVGGPCAN
                     TIAADLKNIGEDCTQGYEQGSSLIELVENGDNVALIVAGYNGADTRAASQAISNFDTS
                     PLAGTRASVDTSSGQVTVLERGDIAAGGQVSGQNRTRTNQTTGTNNTTTANSSSAGNS
                     SR"
     gene            3899..4405
                     /locus_tag="VFF28_02620"
     CDS             3899..4405
                     /locus_tag="VFF28_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44555.1"
                     /translation="MKLFFIAFFLIALTQTAFAQRCAYTSDIDELSMIRDEEVPCPQT
                     VEVWFENTRSEYSDNLTCRYTSTDGKRLSFFIAPEDELRCQPIVSVIFTRTAVLSREA
                     ARAGQQTPLIYSDSQVEQPTTTEPSQPITMFVQTIRDAVDEPLLLGGFILAVILLVIV
                     LGLLIWRR"
     gene            4405..4530
                     /locus_tag="VFF28_02625"
     CDS             4405..4530
                     /locus_tag="VFF28_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44556.1"
                     /translation="MMETYAENMDYMLIVHRDGRKDPNNVVIVTSYDRMDEFMEK"
     gene            4543..5175
                     /locus_tag="VFF28_02630"
     CDS             4543..5175
                     /locus_tag="VFF28_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44557.1"
                     /translation="MPKKGPIEYEIKPENLNIGKEWVDVTLKNITTKALFGLNVKLNS
                     MDTFGLEVLESSRYLPLLGSGQAESVFFRVESYLTTNVYITIDSLRNGDRFHWESPAR
                     TIRVGEDFAELIKFNAGMVGGDITTEASVKSFIDTVNLTYEVWAQGPDGAIEEMEIKD
                     MGEMKDGEVRAVRTGFEPNKKGLYTIDAYLYDGPKRLSHRTDYVMAEEGG"
     gene            5232..5444
                     /locus_tag="VFF28_02635"
     CDS             5232..5444
                     /locus_tag="VFF28_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZX44558.1"
                     /translation="MFIIRLRGVKKDDLMVEVNRKGFRLKAIKRNTTFTAGHCISGET
                     DPKLAKATFQKGILKIMLPRINNELI"
     gene            5453..5620
                     /locus_tag="VFF28_02640"
     CDS             5453..5620
                     /locus_tag="VFF28_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44559.1"
                     /translation="MEQKKECIICGNIYTNLEFPTSFERMVTCGDIKCAGKRRYQKWR
                     ERYDKRARMAA"
     gene            5592..6233
                     /locus_tag="VFF28_02645"
     CDS             5592..6233
                     /locus_tag="VFF28_02645"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HZX44560.1"
                     /translation="MTKGPGWLHRSGKMQGKYHLPDLPYPSESLQPYISKEQLLIHHD
                     KHHKAYVDKANEILDNLAGARKSGAKIEIKGVIKELSFNVGGLHLHSLFWENLRGGGA
                     SPSGRIKAEIENEYGSLDRFKKEFSEGAKAVEGSGWMALAYCSETKRQLLMQIEKHNT
                     NIYPEFKILLLLDMFEHAYYIDYKNDKARYIESFWKIIDWEEVNRRLGGAAAE"
     gene            6281..6718
                     /locus_tag="VFF28_02650"
     CDS             6281..6718
                     /locus_tag="VFF28_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZX44561.1"
                     /translation="MWDPMDALERMQREMDRLVSRVYSGGRDILDFKGREGKTLERYR
                     TPAADIRETERSIIADIELPGVDKRDISLNVTEDSIEVGAQKSREEEKKGRYTYESAK
                     FYRRLPLPAKADAEDVDASFKNGVLRVIVKKSIPEKRKRVDIK"
     gene            6715..8133
                     /locus_tag="VFF28_02655"
     CDS             6715..8133
                     /locus_tag="VFF28_02655"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZX44562.1"
                     /translation="MSSLNQTLLLKSIKSRHFRKFITGSAEILLYWSIVGKAQGVKKE
                     IQKNKYYISAALLSSLNENYNAGNISDYAINRFLSSLLSPNYFANPKKAKAKAAFKEK
                     YGVFPPAFLTISPTNKCNLNCEGCYAESGSMKNEKLPYEVLSKAVKESRDSFGGSFIV
                     ISGGEPFLYEDGGKTLFDLWKEYDDMYFMVYTNGTLINKDTASKLAELGNVSPAISIE
                     GFGVETDKRRGYGVHKSILSAFKTLREHKVPFGVSVTATAQNASILLDDSFYDLYFGK
                     AGALYMWQFHLMPIGRGAESHKNLISPEQRINLYAKWKQILQSKKYLVADFWNSGVLA
                     NGCIAYGREGGYIYIDWNGNIMPCVFIPYFVDNINDLYSNGKTLSDALFSDFFINGRN
                     WQKSYSFTENPKNLLLPCSIRDHYDNFRKNIVTKGVKPENEEAGKALFSQSYFQMMGD
                     FDRELKERADAIWKQEYGGEIS"
     gene            8280..8426
                     /locus_tag="VFF28_02660"
     CDS             8280..8426
                     /locus_tag="VFF28_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
                     /protein_id="HZX44563.1"
                     /translation="MVVSTTLLIGLLNIIIGLLILLIPGMLRFLVGAYFLLSGLLMIV
                     LPLI"
     gene            8437..8667
                     /locus_tag="VFF28_02665"
     CDS             8437..8667
                     /locus_tag="VFF28_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaB family protein"
                     /protein_id="HZX44564.1"
                     /translation="MPYKDVSELPKQVRDNLPKHAQKIYMKAHDSALEEYKDPGKRRG
                     RASLEETAHKVAWAAVKKEYMKSGGKWVRAND"
     gene            8684..8872
                     /locus_tag="VFF28_02670"
     CDS             8684..8872
                     /locus_tag="VFF28_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44565.1"
                     /translation="MADRYRGESGFRKTMDEVKDNMASMRANFEDKVAEEPMKSMIKA
                     FVIGAVVGGALGMLMRRR"
     gene            8869..9174
                     /locus_tag="VFF28_02675"
     CDS             8869..9174
                     /locus_tag="VFF28_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44566.1"
                     /translation="MSISDIIEEIRDFFGRAEDRVESTVMRILDRAKDEVRLEAHRVK
                     RWIFASIFQAAFYILGIVLLLGALTLFAGRWLPFDLVLLIEGVLLIYAALMLHLVRP"
     gene            9182..9604
                     /locus_tag="VFF28_02680"
     CDS             9182..9604
                     /locus_tag="VFF28_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44567.1"
                     /translation="MPKKKKDDNDIKIAIDRVMKIDQSQREESIEEEVEKAAPAHHIE
                     TPNPAAPVTIEPAAPYRAFYEMKITYIPEPSIPNIALYHSEDKEALVEDHEQPGSPLE
                     EAERSFEQGKVLPDKISGQETLQGGEMRKLLTKKGHEP"
     gene            9594..10526
                     /gene="ligD"
                     /locus_tag="VFF28_02685"
     CDS             9594..10526
                     /gene="ligD"
                     /locus_tag="VFF28_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HZX44568.1"
                     /translation="MNLERLGRKSAMPEWIAPMLATLTDRRFSHSGWIYERKLDGQRC
                     LAFKDGAVKLYSRNRKNISLNYPEIVLGLENTSHQFIIDGEIVALEGDRTSFSLLQPR
                     MQGTIDKTPVFYYVFDIIYLDGIDLSQALLIDRRKVLETLKFQGPVRILAHSDEGLKF
                     YEEACSKGWEGLIAKDKGSCYHPGRSSCWLKFKCLQEQEFVIGGYSDPLGSRTSFGAV
                     LVGYYEGDKLIYAGKVGTGFSEQVLKKMGEAFKLIDQKEKSFESDIKEKGVHWVRPVL
                     VCQVKFTEWTPDGKLRHPTFAGLRFDKDPKDVHL"
     gene            10513..11148
                     /locus_tag="VFF28_02690"
     CDS             10513..11148
                     /locus_tag="VFF28_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013553279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HZX44569.1"
                     /translation="MSIFKEETIFKDRVDAGQKLSRVLSKYQNKNLLVLGIARGGAIV
                     GKEVARNLNADFDIIIAKKLPYPDDPEAGFGAVAEDGSYVFLPDMSPGKEEVERIKDE
                     QVEEIKRRIAVLRGGRDIKQIEGKVVILVDDGMAMGMTLQAAIKMCRKKKASFVVAAV
                     PVTSFGTSELVRPMVDELIVLEMPAFFIAVAQGYEHWYDVSDEEVIEVLKR"
     gene            11145..11918
                     /locus_tag="VFF28_02695"
     CDS             11145..11918
                     /locus_tag="VFF28_02695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HZX44570.1"
                     /translation="MKTVWKGAISFGLVSIPINLYSAIEGKALSFRLLHKPDHQPIKY
                     KRFCQDGHEVPWNEITKGVEIAKDRYYEVDGEELEKLKPEKSDVVDIFEFVDARQVDP
                     IYFNSHYYAAPQRQKDKAFFLFKEVLSQSAKVAIGKFVMRQKEYISAIESYKSGLLLT
                     TLNYGYEIRDMGAIEELSSAPKLREEELSLAKQLIDRLYKEEFDIRQFKDTFAEQLKE
                     LLRRKEKGEAITVEHKPQAEGKEQDIIKALKQSLREAEA"
     gene            11915..12049
                     /locus_tag="VFF28_02700"
     CDS             11915..12049
                     /locus_tag="VFF28_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44571.1"
                     /translation="MRRCDPKLHEKDAGYGVCGQRRRDKKLCDEEGSDDTMKKFTCDK
                     "
     gene            12046..12303
                     /locus_tag="VFF28_02705"
     CDS             12046..12303
                     /locus_tag="VFF28_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZX44572.1"
                     /translation="MMAKIRTNHGDIEVRDGTNLGDACEKIGLNVVCRVGVCGLCQLR
                     VVAGQENLTELTQPEIDKGMDKDNRLACQCSIKSGEIEVSF"
     gene            12304..12693
                     /gene="msrB"
                     /locus_tag="VFF28_02710"
     CDS             12304..12693
                     /gene="msrB"
                     /locus_tag="VFF28_02710"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HZX44573.1"
                     /translation="MDEAEIKKKLTPEEYHILRDEGTEPPFSGEYNYNKKEGSYHCKV
                     CGQKLFSSEKKYDLGTGWPSFFDAVKGAVVKRHDEKHGGTEIACARCGSHLGHIFDDG
                     PKPTGKRYCVNSLSLDFKEDKSARGEG"
     gene            12693..13142
                     /gene="msrA"
                     /locus_tag="VFF28_02715"
     CDS             12693..13142
                     /gene="msrA"
                     /locus_tag="VFF28_02715"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HZX44574.1"
                     /translation="MNEKATFGAGCFWHVEEQFSKVGGVIETTAGFMGGEKKDPTYEE
                     VSTGKTGHAEAVQVEYDPSKIAYDDLLDIFWNIHNPTLLNQQGADVGTQYRSVIFYHS
                     ESQKRIALESKSRLPNASQVVTEIVPVGVFYSAEEYHQKYIKRRGLC"
     gene            13179..13595
                     /locus_tag="VFF28_02720"
     CDS             13179..13595
                     /locus_tag="VFF28_02720"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZX44575.1"
                     /translation="MGEGNSQSGIMLIGQNPGETEEKLGRPFVGRTGRFLDKVFAEHG
                     IDRKNLYITSVVKCRTQGNRKPSPAEVKSCLPILTEEIKNIKPKVIVLMGKVAQGIPR
                     LSGIDYIETVHPTAAMRFPKYRKKFMDDIAKLESYL"
     gene            13592..14596
                     /gene="amrS"
                     /locus_tag="VFF28_02725"
     CDS             13592..14596
                     /gene="amrS"
                     /locus_tag="VFF28_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HZX44576.1"
                     /translation="MKEAMFYNKLKDKVVNCRLCPHYCVIDPDGIGKCGVRQNIDGKL
                     FSLVYGKIISSAVDPIEKKPLFNFLPGTKSYSIATAGCNLFCEFCQNWEISQLPKTEG
                     VRGFDMEPAEIVEDALKKGCQSIAYTYTEPTIFLEYALDIAKIARKKKLKNIFVTNGF
                     ITTEAAAEASKVIDAANVDLKGFTDDYYKKVCGARLKPVLDAIRTYYDNGVWLEITTL
                     IVPGHNDSPEMLTRIAEFIASVDKKIPWHISRFFPHYKMLSAKPTPVETVIHAVNIGK
                     KIGLKYIYGGNLMSDEHSQTLCPSCGEEVISRRGFFVEAKHIKGNVCEFCRKKIDVVE
                     "
BASE COUNT         4659 a         2830 c         3783 g         3509 t
ORIGIN      
        1 ttttcatgcg aggagagtat ggaacgatca actcaggaag ggacgtcagc agatacccat
       61 tagctgattt aactgcgact tcaatcccat catagatcct cctcaaacca gcaagcaact
      121 ctgctttttc agcttctaca actggatttt ttgtcaaggg aactctatga tggggcgagg
      181 gaggacaaaa acaatagtac atatgttcac ccggatttct gaattttgtc tcttccagag
      241 gaacagacga atgccctgcg aagtctactt cgacaattct cggatcgact gtcacaggtg
      301 tccacactcc ggaatttgct gcctgcctga tggcatcgaa ccttgctccg tcgtagcctc
      361 ttaccagcgg ggtagaatct atttcatccg cccttatcct gccaaggatt attgctgaca
      421 tataagcagc tatcaaccca ttcatattca atggctcatc atccacgctt tggaggacaa
      481 tggtgcctat atggctgccc cgattgaatt ctgtctcctg gtgcctgctg gcaatcattg
      541 taaccataca acccttttgt tgccacttta cttatatacc tttgtctata tttataaata
      601 tactttatgt cggatatata ctgtaattac aataattggc ataataattt aagagcgaca
      661 gatactattt ttcatcatga aaaagaaaat ttgggaaaca tcaacaagag atgaactgca
      721 gaacgcaggt gcaagattta actcttttgg taacgacgaa gatttctatg ccatactgaa
      781 tggaggacag atctttgtta agagatttgg cgactcctat aggcagatat aatcaattct
      841 tggataactt gtgtcactct gtttcaatta gtacaaatct ttatattatt gggtaagata
      901 atcaatttga taataatctt taattattat tatcaatttg gttataaata aaaaaattgg
      961 gggtggtagt aatggaatat ggtgaaacag atgacgaaag catatataat gaggaagtta
     1021 gggacgaact gctcgatcgt gatgcccttg ccgcaagtga ggtagggttt atgataggct
     1081 atgatgaggc ctatgatgcg gaggatgggg ttgtataggc aataatgcta tttttcttca
     1141 ttttttttgg gtttaactac taagcaggac tactaaagtt ttttatatgt ttacggctaa
     1201 ataagtattt atgacacttt ttatagttaa tgattatcaa aatggcaata tattgccaga
     1261 aaaccataac aagcgttatg gaccggtcaa taaaaaaatc aagggggtgt ctttttatga
     1321 accaatcaag aacaatactg gcgatgcttg tcatctttct ttttggaagt gcaaacatct
     1381 cctttgcagt ggacctggga gattttccgg ctcctttcat ccaaggcaac gagatgagag
     1441 gatatcttgt ggtaggcgac cgtgccagcg cagaggatat cgtcggagtg agtgacatag
     1501 ccgtcagcct tcagtatgct atatccggcg gatcaggcag cggcaggtct aatttcagcg
     1561 tctcaggagc gacttggcag gtggggacaa cttcaagtca gctagagatg gcgcaaaaca
     1621 cagatacatt cagcggagag acgataggag atatcagaac cactatagac gagacagagc
     1681 ttagcctgct tacagatggc agcatcacaa cagaaaaagg gacatcaaga tacaaccaat
     1741 atcttatctt tccaagcgat aaccagtcat catatgtaat ctttgcagaa aatgacaatg
     1801 atactgcttc cgatttcctt tattttagga gcgggcaagg gataggaagg tacagtcttg
     1861 aatttgcaac aagcctgcaa agcgatgttg aagacagtgc aggtgcaagg actgcaacag
     1921 ggacctatct tggtgactat gaggatgaaa taataactat catgggcaag gaatttaata
     1981 tagtcacagc aagaaggaca agcgcaacca caaacagcat agagcttacc ttgatgggag
     2041 gtgctgtgag agataccctg gaagagggag agacaaaaac ctacactgta gagggaaagg
     2101 aatatgaggt cacggcaaca gccataactg acacagctcc tatctatgca agattcaata
     2161 taaacggaga gtctgtaagg tccttatctg acggcgagac agctacattg tctgatggga
     2221 cagagatagg ggtagtcgac atcgtgccaa acgaggcagg cgatccgaca caggacctgg
     2281 tcgaattttt cgtcggcgca tcaaaactgt tcctgaagga cagcgatgtc accaacactg
     2341 ccagcagcaa tgcccttgaa gtgggtacag agagggtaga tgatagtgct gtcatcatca
     2401 caggctccaa cgacaacgcc acattcagca tagatacgat ccaggtaaat ataactgcag
     2461 atgatgattt ctatgtaggc gcaggccaga aactttccca gttcatggac gagcctgagg
     2521 ctcttcttgg agtttgggat atccaatacg gagggcttgc cagccttggc actgaaacat
     2581 acaggcttag atcatctggc tctgaccagt atgacctggt atttgtcgac ggtgacggcg
     2641 acagcgcaaa tctgcccctg gtatactcca atgtttccag ccagataaag cttggcgaca
     2701 gggatgatga tcttatagta aatggatccc ttgtcataag caagaatgat tattttgtca
     2761 taagttatgg cgggcaacaa gcaggggata ggaagactta tgcattcagg tacaggggag
     2821 cagacaaggt agcagctgga gagacagcgc aggtgaggct tcagaacctt ggaacaggcg
     2881 atacctctga aaataccttc agggacaata caggagacac tgcagatgca acgctcaagg
     2941 taggcggttc tacattcaac atcatcaatg cgagcgctgc aacaagcaat gactttgata
     3001 taatggtgga ccttgacgga gacggcacca cagcagggga tactgatata atccctatag
     3061 acaccaattt tgggcagaca ataaccttta cagtcggcac acctacatat cttgtggtcg
     3121 ctttcacaac accaaacaca aatgactatg ataacatagc gcctgctgct gtgacgatga
     3181 acataaccgc ggcctcagac agagaggttt cctttgctga atcttcaggc agtactatca
     3241 actggataag ccccaccgag gaggacaaca ttaactatgc ctacacaagc atgggagcaa
     3301 gatacaggtg gtcaaatcca tcaaacgacc ctgatgacct tgaagttagc tatcccaggg
     3361 tgcaaaggct accgcttgtg tatatcagcg gaagcaatgc aacaactgca acagtaactt
     3421 cgggcgagac aacaacagca ggcaaccgca tagaggttgg cgctgtcagg cttgcaagcg
     3481 aggtccagac gccttcagcc cagaacctca tcatcgtcgg tgggccatgt gcaaatacaa
     3541 ttgcggcaga cctcaagaac attggtgagg actgcaccca aggctacgaa caagggtcaa
     3601 gcttaatcga acttgtggag aatggagata atgtggcatt gatagtcgct ggatacaacg
     3661 gagctgatac aagggccgca agccaggcga tatccaattt tgacaccagc ccgttagcag
     3721 gtacaagggc aagcgtggac acctcaagcg gacaggtgac tgtgcttgag agaggagata
     3781 ttgctgcagg aggacaagtg agcggacaaa acaggacaag gacgaaccag acgacaggca
     3841 caaacaatac aaccacggca aacagttcaa gcgccggaaa ctcaagcagg tgaaaagcat
     3901 gaaactattt tttatcgcat tttttttgat tgcgcttacc cagactgcat ttgcacagag
     3961 gtgcgcctat acatctgaca tcgatgagct gagcatgata cgtgatgaag aagtgccttg
     4021 cccgcaaaca gttgaagtat ggtttgagaa taccagaagc gagtattcgg acaacctaac
     4081 gtgcaggtat acttcaacag atggcaaacg actatctttt tttatagcac cagaggacga
     4141 gctgcgctgc cagcctatag tgtcggtcat ctttacaagg actgctgtat taagcagaga
     4201 ggccgcaaga gccggccagc agacgccact aatctactct gacagccagg tagagcagcc
     4261 tacaactaca gagccgtcac agcctataac aatgtttgtt cagacaataa gggatgctgt
     4321 cgatgagccc ctgctgctcg gcggatttat cctggcagtg atacttttgg tcatagtgct
     4381 tggactgtta atttggagga gatgatgatg gagacatatg cagagaacat ggactacatg
     4441 ctcatagtcc acagggatgg aagaaaggat ccaaacaacg tggtgatagt gacgagctat
     4501 gataggatgg atgagtttat ggaaaaatag aatgatgaca aaatgcccaa aaaaggacct
     4561 atagaatatg agataaaacc agagaacctt aacatcggaa aggaatgggt agatgtaact
     4621 ctaaagaata tcacgaccaa ggcccttttt ggacttaatg ttaagctaaa ctccatggat
     4681 actttcggcc ttgaagtgct tgagtcatcc agatatcttc cacttcttgg atccggacag
     4741 gcggaatccg tcttctttag ggtcgaatct tacctgacca ccaatgttta cattactatc
     4801 gattcgctca gaaacggcga caggttccac tgggaaagcc ctgccaggac tataagggta
     4861 ggggaggatt ttgccgagct tataaaattc aatgcaggca tggtcggagg agacataacc
     4921 acagaagcat cggtcaaatc tttcatcgac actgtcaacc tgacctatga agtctgggcg
     4981 cagggtcctg atggggctat cgaagagatg gagataaaag atatgggaga gatgaaggat
     5041 ggggaggtaa gggcagtaag gacaggattt gagcctaata aaaaggggct ttatactatc
     5101 gacgcttatc tctatgacgg cccaaaacgc ttgagccaca ggacagatta tgtcatggcc
     5161 gaagaggggg gataaacatc aaattcatca gattaaccca ggtagaatca tcatactgcg
     5221 aagggaaata tatgttcatc ataagactta gaggagtaaa aaaagatgac ctcatggtcg
     5281 aggttaaccg gaagggcttt cgcctaaagg ccataaagag gaacacaacc tttacagcag
     5341 gacactgcat ctctggtgag acagatccta aacttgcaaa agccacattc cagaaaggaa
     5401 tactcaagat tatgctgcca aggataaaca atgaacttat ctaggaggta aaatggagca
     5461 aaaaaaggaa tgtataatat gcgggaacat ctatacaaat ctggagtttc cgaccagctt
     5521 tgaaaggatg gtaacttgcg gcgatatcaa atgcgccggt aaaagaagat accaaaaatg
     5581 gcgagagaga tatgacaaaa gggccaggat ggctgcatag gagcggaaaa atgcaaggaa
     5641 aataccacct gccagacctg ccataccctt ctgaaagcct tcagccatat atatcaaagg
     5701 aacagctgct catccatcat gacaagcatc acaaagcgta tgtcgataaa gcaaatgaga
     5761 tacttgataa tcttgctggc gcaaggaaat ccggggccaa gatagagata aagggagtga
     5821 taaaggagct gtcattcaat gtaggtgggc tacatctgca ttcactgttt tgggaaaatc
     5881 tcagaggcgg aggagcgagc ccttcaggaa ggataaaggc agagatagag aatgaatatg
     5941 gcagtctgga caggttcaaa aaggagttca gcgaaggggc aaaggctgtc gagggctctg
     6001 gatggatggc tcttgcctat tgctcagaga ccaaaaggca actgctcatg cagatagaaa
     6061 agcataatac aaacatctat ccggaattta agatactgct cctgctcgac atgtttgagc
     6121 atgcttacta cattgattat aagaacgata aggcaagata tattgaatca ttctggaaaa
     6181 taatagactg ggaagaagtt aaccgacggc ttggaggagc cgctgctgaa tgacacaatc
     6241 agggttatac tagagatgct tgtttttgga ggtgattttt atgtgggatc ctatggatgc
     6301 tctggaaagg atgcaaagag agatggatag gctggtaagc cgcgtatact ccggtggaag
     6361 ggatattctg gattttaagg gccgtgaagg aaaaactttg gagaggtata gaacccctgc
     6421 cgcagacata agagagacag agcgcagcat aatcgcagac atcgagcttc caggtgttga
     6481 caaaagggat atatccctca atgtcactga ggacagcatc gaagtaggcg ctcaaaaatc
     6541 aagagaagag gagaaaaagg gaagatacac ctatgaaagc gccaagttct atagaagact
     6601 gcccctacct gcaaaggccg atgctgagga tgtggatgcc agcttcaaga acggagtgct
     6661 tcgcgtaata gtaaagaaat caatacctga aaaaagaaaa agagtggata taaagtgagc
     6721 agcctcaacc agacattgct gctaaaatcg ataaagtcaa ggcattttag aaagtttatc
     6781 actggcagcg cagagatcct gctgtactgg tcaatagtgg gcaaagccca aggcgtgaaa
     6841 aaagagatcc agaaaaataa gtattacata tcagcagcat tattaagctc attaaatgag
     6901 aattataacg caggcaatat ctctgattat gccataaacc ggtttctttc aagcctgcta
     6961 tcacccaatt attttgccaa cccaaaaaaa gccaaggcaa aagctgcgtt caaggaaaag
     7021 tacggtgtgt ttccacctgc ttttttgact atcagcccca ctaacaagtg caatctaaac
     7081 tgtgaaggat gctatgcaga atcaggctct atgaaaaacg agaaattgcc gtatgaagta
     7141 ctgtcaaaag cggtaaaaga gtccagagac agctttgggg gaagctttat tgttataagc
     7201 ggaggagagc cctttttgta tgaagacgga ggtaagacgc tgtttgacct ttggaaggaa
     7261 tatgacgata tgtatttcat ggtctatacc aatggaacct tgataaacaa ggatacagca
     7321 tcaaagcttg ctgaacttgg gaatgtcagc cctgctatct caatagaagg atttggcgtc
     7381 gagactgaca aaagaagagg ctacggcgtc cataagtcta tcctcagtgc gtttaaaacc
     7441 cttagggagc acaaagtgcc ttttggtgtt tcggtcacag cgacggcaca gaacgccagc
     7501 atacttctgg atgattcatt ttacgacctt tactttggca aggctggggc attatacatg
     7561 tggcagttcc atcttatgcc tataggcaga ggggcagagt cgcataaaaa tctgataagc
     7621 cctgagcaaa ggataaacct gtatgcaaaa tggaagcaga tattacaatc aaaaaaatat
     7681 ctggtggctg atttctggaa cagtggtgtg cttgccaatg gatgcatagc atacggcagg
     7741 gaaggaggat acatctacat cgactggaac ggaaatatca tgccttgcgt gtttatccct
     7801 tattttgtcg ataatatcaa cgacctttat tcaaacggaa aaacactgtc tgacgccctg
     7861 ttctctgatt tttttatcaa cggaaggaac tggcaaaaaa gctattcctt cacggagaat
     7921 ccgaagaatc tgctgcttcc ctgctctata cgtgaccatt atgacaattt cagaaaaaat
     7981 atagtcacca aaggcgtcaa gccagagaat gaggaggcag gaaaggccct ttttagccag
     8041 agctattttc agatgatggg agattttgat agggagctta aggaaagggc agatgcaata
     8101 tggaagcagg aatacggcgg agagatatca taaaaatcag ctgaaagtgt cttaacggtg
     8161 cactaaagtg catggtttgt ccagacactt tctccaagcg gatctttagg atgctagaga
     8221 gacgagccta atatccacgg actaaaatcc gcagtctcag acataaaacg aggtgatggg
     8281 tggtggtatc tacaacttta ttgatcggtc ttttgaacat aatcatcggg cttctaatac
     8341 tcctgatacc tggaatgcta agatttcttg tcggagcgta ttttttgctt tccgggcttc
     8401 tgatgatagt gctgccactg atataggggg ataaatatgc catataagga tgtcagcgaa
     8461 cttcccaagc aggtgaggga caatcttccc aagcatgctc agaagatata catgaaggcg
     8521 catgacagcg cacttgaaga gtacaaagat ccaggcaaaa ggagaggcag ggcttccttg
     8581 gaagagaccg cacataaggt cgcctgggct gcagttaaga aggaatatat gaaatcaggc
     8641 ggtaagtggg taagagcaaa tgattaattt tgggggtgaa aaaatggcag acagatatag
     8701 aggagaatca ggctttcgta agacgatgga tgaagtgaag gataatatgg ccagcatgag
     8761 ggctaatttt gaggataagg tggcagaaga gccgatgaaa tcaatgatca aagcatttgt
     8821 catcggtgct gttgtaggcg gagcgcttgg gatgcttatg aggagacgat gagcatatca
     8881 gacatcatag aagagataag ggattttttt ggcagggctg aggacagggt ggagtcgaca
     8941 gtcatgcgca tccttgacag ggcaaaggac gaggtgaggc ttgaggcgca cagggtaaaa
     9001 agatggatct ttgcatctat ctttcaggct gcattctaca tattaggcat cgtgctgctt
     9061 ttaggagcgc taaccctttt tgcaggaaga tggctgccat ttgacctggt attattgata
     9121 gaaggagtgc tgctcatcta tgcagcgctg atgctccatc tcgtaaggcc ctagagcgca
     9181 aatgccaaag aaaaagaaag atgataatga cataaagatc gcaatagata gggtgatgaa
     9241 gatagatcaa tcacagaggg aagagagcat cgaagaagag gtagagaagg ctgcaccggc
     9301 acatcacata gagacgccca atcctgctgc tcctgtgacg atagagccgg ctgcacctta
     9361 cagagctttt tatgagatga agatcactta tatacctgag ccatcgatac caaatatcgc
     9421 tctgtatcac agtgaagata aagaagcctt agttgaagac catgagcagc caggaagccc
     9481 tcttgaagaa gcagagcgct cttttgagca gggaaaagtc ctgccagaca agatctcagg
     9541 acaggagacg ctgcagggag gggagatgag aaagctgctc accaagaaag ggcatgaacc
     9601 ttgagagact gggaaggaag tcagcgatgc ctgagtggat agcgccgatg cttgcaaccc
     9661 ttacagaccg caggttttca cacagcggtt ggatatatga gaggaagctc gacggccaaa
     9721 gatgcctggc attcaaggat ggggcagtca agctttactc acgcaacagg aagaatatca
     9781 gtttgaatta tcctgagata gtattggggc ttgaaaacac aagccatcaa ttcataattg
     9841 acggagagat agtcgccttg gagggtgaca ggacaagttt ttctttgctc cagccaagga
     9901 tgcagggaac aatagacaag actcctgtct tttattatgt ttttgatatc atctatcttg
     9961 acggcatcga tctatctcaa gctttgctta tcgatagaag aaaagtgctg gagaccctca
    10021 agtttcaagg gccagtcaga atccttgccc actctgacga aggacttaaa ttttatgaag
    10081 aggcatgttc aaagggatgg gaaggcctta tcgcaaaaga taagggctca tgctaccacc
    10141 caggaagatc aagctgctgg ttaaagttca aatgcctgca ggaacaggag tttgtcatag
    10201 gagggtatag cgatcccttg ggaagcagga catcgtttgg agctgtgctt gtgggctact
    10261 atgaggggga caagctgata tatgcaggaa aagtggggac cggctttagc gagcaagtgc
    10321 tcaaaaagat gggggaggct ttcaagctga tagatcagaa agaaaaaagc tttgaatcag
    10381 acataaagga aaagggcgtg cactgggtga ggcctgtgct cgtgtgccag gtgaaattta
    10441 ctgaatggac acctgatgga aaacttaggc atcccacttt tgcaggccta aggtttgata
    10501 aggatccaaa agatgtccat ctttaaggag gagacaatct tcaaggacag ggtcgatgcg
    10561 ggtcagaagt taagcagagt tctgtctaaa taccagaata aaaacctgct tgtccttggt
    10621 atcgccagag gcggagcgat agtcggaaaa gaggtggcaa gaaacctaaa tgctgatttt
    10681 gatataataa tcgcaaaaaa gctcccttac ccggatgatc ctgaagcagg gtttggggct
    10741 gttgccgaag atggcagtta tgtttttctt cctgatatgt caccaggcaa agaagaggtt
    10801 gaaaggataa aggacgaaca ggtcgaagag ataaagagaa ggatagcggt ccttagggga
    10861 ggaagggata tcaagcagat agaaggaaag gtcgtcatac ttgtcgatga cggcatggct
    10921 atgggaatga ccctacaggc agcaataaag atgtgcagga agaaaaaagc gtcatttgtt
    10981 gtagctgctg ttcctgtgac ctcctttgga acctctgaat tagtcaggcc tatggtggat
    11041 gagcttatcg tccttgagat gccagctttt tttatcgctg tagctcaggg ttatgagcat
    11101 tggtatgatg tttcagatga agaagtgata gaggtcctga agagatgaaa accgtctgga
    11161 aaggagctat atcctttggg cttgtatcca tcccaataaa tctatattct gcaatagagg
    11221 gcaaagcact aagctttaga ctgctccata agccagacca tcaaccaata aaatacaaac
    11281 gtttttgcca agacggccat gaggttccat ggaacgaaat aacaaagggt gtggaaatag
    11341 caaaggacag atattatgag gtggatggag aagagcttga aaaactcaag cctgaaaaat
    11401 cagacgtagt agatatattc gagtttgtcg atgcacgaca ggtagaccct atatatttta
    11461 attcacacta ttatgctgcg ccccaaaggc aaaaggataa ggcttttttc ctgtttaaag
    11521 aggttctttc gcaaagtgca aaggttgcca tcggcaagtt tgtgatgagg cagaaagagt
    11581 atatctctgc catagagtcg tataaaagcg gacttctgct taccacactt aactatggct
    11641 atgaaataag ggatatgggg gccatcgagg agctttcttc tgcgcctaaa cttagggaag
    11701 aagagctttc tttagcaaag caattgatag acaggctgta taaagaggaa tttgatatca
    11761 ggcagtttaa agatacattt gcagagcagc ttaaggaact tctcagacgc aaggaaaaag
    11821 gggaagcaat aacagtagag cataagcctc aggcagaagg aaaggagcag gatataatca
    11881 aagcgctaaa gcaaagctta agagaggcag aggcatgagg aggtgcgatc ccaaacttca
    11941 tgagaaagat gcaggctatg gggtttgtgg acagagaaga agggataaga agttatgtga
    12001 tgaagaagga tctgatgata ccatgaagaa gtttacctgt gataaatgat ggctaagata
    12061 agaacaaacc atggggatat agaagttagg gacggcacaa accttggtga cgcctgtgag
    12121 aagatagggc ttaatgttgt ctgcagggtg ggagtttgcg gtctttgcca gctgagggtg
    12181 gttgcaggcc aggagaatct caccgagctt acgcagcctg agatagacaa gggcatggac
    12241 aaagacaaca ggctggcttg ccaatgcagc ataaaaagtg gagagataga ggtgagtttt
    12301 tagttggacg aggctgaaat aaagaaaaaa cttacgcctg aagaatacca tattctacgg
    12361 gacgagggga cagagcctcc tttttcaggt gaatataact ataacaaaaa agaaggcagt
    12421 tatcactgca aggtgtgcgg gcagaagttg ttttcctctg agaagaaata tgacttaggt
    12481 acaggctggc cgagcttttt tgacgctgta aaaggagcag tcgtaaagag acatgacgag
    12541 aagcatggcg gaacagagat cgcctgtgca agatgcggca gccatctggg acatattttt
    12601 gacgacggac caaaacccac aggcaagaga tactgtgtca attcattgag cctggatttc
    12661 aaggaggata aatcggcaag aggggaggga taatgaacga gaaagctaca tttggagcag
    12721 gatgcttttg gcatgttgaa gaacagttca gcaaggtagg gggggttatc gaaacaacag
    12781 ctggctttat gggcggggaa aagaaggatc ctacctatga agaggtatcg acaggaaaaa
    12841 caggccatgc tgaggcagta caggtcgaat atgacccttc aaagatagct tatgatgacc
    12901 tacttgatat tttttggaac atacacaacc caacacttct taaccagcag ggagctgatg
    12961 tgggaacgca gtatagatcg gtcatatttt atcatagcga aagtcaaaaa aggatagctc
    13021 ttgaatcgaa aagccggctt cctaacgcaa gccaggtcgt aacagaaata gttccggtcg
    13081 gtgtttttta ttcggctgaa gaatatcacc aaaaatacat taaaagaagg ggattatgct
    13141 aagtgggcgc taaatgcaca aggtgtataa agccagtgat gggtgagggc aacagccagt
    13201 caggaataat gctcatcgga caaaatccag gagagaccga ggagaagctt ggaaggccat
    13261 ttgtcggaag gacagggaga tttcttgata aagtatttgc tgaacatggg atagatagga
    13321 agaatctgta tataaccagt gttgttaaat gccggacaca gggtaataga aaaccttctc
    13381 cagcagaggt aaagagctgc ctgcctatac tcacagaaga gataaaaaat ataaagccca
    13441 aagtcatagt gctcatggga aaggttgcac agggcattcc caggctttcg gggatagact
    13501 acatcgagac tgtgcatccg accgctgcta tgagatttcc aaagtaccgc aagaagttta
    13561 tggatgatat tgccaagctt gagagctacc tatgaaggaa gcaatgtttt acaataagct
    13621 taaggataag gtggtaaatt gcaggctgtg ccctcattat tgcgtgatag accctgatgg
    13681 gataggcaag tgtggtgtcc gccagaatat cgatggaaag ctgttctccc ttgtttatgg
    13741 caagataata tcttctgcag ttgatcccat agagaaaaaa ccgctcttta attttctgcc
    13801 tggaacaaaa tcttacagta tcgctacagc aggatgcaac ctattttgcg agttctgcca
    13861 aaattgggag atatcgcagc tgcctaaaac agagggagta agaggatttg acatggagcc
    13921 tgcagagata gttgaagatg cgctcaagaa aggctgccaa agcattgctt atacctatac
    13981 agagcctact atatttcttg agtatgcact tgatatagca aagatagctc gtaagaaaaa
    14041 gctaaaaaat atatttgtga caaacggatt cattacaact gaagctgctg ctgaggcaag
    14101 caaagtgata gatgctgcaa atgttgatct caagggattt actgatgact attataagaa
    14161 agtctgcggt gcaaggctta agcctgtgct ggatgctata cggacgtatt atgacaatgg
    14221 agtgtggctt gagataacga cgcttatcgt tcctgggcac aatgacagcc ctgagatgct
    14281 tacgcgcatc gctgaattta tcgcctctgt cgataaaaag attccttggc atatttccag
    14341 gttttttccg cattacaaga tgctaagcgc taagcctaca cctgttgaga ctgtgataca
    14401 tgctgtgaac ataggcaaga agatagggtt gaagtatatt tatgggggaa atctcatgtc
    14461 tgatgagcac agccagacac tttgcccaag ctgcggcgag gaagttattt ccaggcgagg
    14521 gttttttgtg gaggcaaaac atatcaaggg gaatgtatgc gagttctgca ggaagaagat
    14581 tgatgttgta gagtgaattc ttataaactt tatataaaat ttaaggcata gctatttgtc
    14641 ttaaatttct tagcagaaat ccccaccact tgtggttggg gatgaatgcg taacacttat
    14701 aggatggcta attctgtgat tatctgttgg taaaatgatg agcctcaaaa aagaggtgag
    14761 gcccaatgac actttaccaa a
//
LOCUS       DASRUK010000056         7912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_713404, whole genome shotgun sequence.
ACCESSION   DASRUK010000056 DASRUK010000000
VERSION     DASRUK010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 7912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7912
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713404"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VFF28_02730"
     CDS             <1..653
                     /locus_tag="VFF28_02730"
                     /inference="COORDINATES: protein motif:HMM:NF027593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4931 domain-containing protein"
                     /protein_id="HZX44577.1"
                     /translation="HEVIVDTNSHDKQLWDKDKNHISELLKVIAKRITEMEKVKCIKY
                     VLAFKNHGEKAGTSILHSHVQLASVAMIPTEIKTKIAAVNEHKRCPYCEIIKIESKSK
                     RKCFENKDFVAFAPYASRFNYEIWFFPKRHVTRMENLKESEISSLAELIHKTLTKLKS
                     INASYNLFIQYSPGKEDLHFHIEITPRIATWAGFEFSSGIVINSVSPESAAGFYRS"
     gene            complement(650..2143)
                     /gene="gpmI"
                     /locus_tag="VFF28_02735"
     CDS             complement(650..2143)
                     /gene="gpmI"
                     /locus_tag="VFF28_02735"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HZX44578.1"
                     /translation="MNKVILIIRDGWGYRKEHKDNAIFEAGTPVTDRLMKEYPNTLIK
                     ADGEAVGLPEGYQGNSEVGHMTIGAGRTIFQPMVRINNSIADGSFYKNKAFLGAISNC
                     KKNKSKLHLIGLLQSEGVHSHEAHLYALLELCKKESFNDVYIHVITDGRDSPPGDGLK
                     HIQKLVEKLKELGFGKITTLSGRYYGMDRDKRWDRTKKAYDTIVLGEGGEFSDVLGIV
                     GWCYANRETDEFIVPIRLKGYNGIKDKDSVIFFNFRTDRPRQLTQAIVEKDFAGWKRG
                     PLDVFYVTMTEYYKPMNAHAAFLDQSLANLLGEVISRHGIKQLRISETEKYAHVTFFF
                     NGQRETPYDGEDRAMIPSPKVATYDLKPEMSAYEVTERLVSEIETDKYGFIVVNIVNG
                     DLVGHTGVVPACLKAVKVVDECVGKIVTAGLDKNYTMLIFADHGNIEDQSPEWKTSHT
                     IDPVPLVLVSKDKKHALKKGGLGDIAPTVLNLLGIEKPKEMDGKSLI"
     gene            2321..2656
                     /locus_tag="VFF28_02740"
     CDS             2321..2656
                     /locus_tag="VFF28_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44579.1"
                     /translation="MLMKFDPGLTRQEIWAEGSELATLLGYDRDEHGKRVHSWPKERK
                     IHVATLDNPVLVTDSYKICQFPSTLRDTVFALFASPTKTTEYKGTSLEFEQRRYPGVW
                     GPSIDTLLM"
     gene            2653..3240
                     /locus_tag="VFF28_02745"
     CDS             2653..3240
                     /locus_tag="VFF28_02745"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HZX44580.1"
                     /translation="MRALDPSELTAVQTACEIGPGSGYVSLYVRNNAPHLEQIDLVDL
                     SDSAIACAREVIKDRRATYAVGDGREFLVGKKYGLVYCNPPYILRPKSIDDNPYEGIC
                     LLVYLVTHADEFLNKGGRLVTNVSSLAEPVTTPLFADTGIRTIDQMHVPLKVFNVLNN
                     PEWMDYLLSEKGLRKESRDGYDYWHTISIAEVRKR"
     gene            complement(3495..4769)
                     /gene="eno"
                     /locus_tag="VFF28_02750"
     CDS             complement(3495..4769)
                     /gene="eno"
                     /locus_tag="VFF28_02750"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HZX44581.1"
                     /translation="MSKIKSVKAREILDSRGNPTVECDIVTEKGLFRASVPSGASAGV
                     HEALEMRDGDNKRFLGKGVHNAVRNVNHEIAETLVGIEASDQIDIDDDLIELDSTENK
                     ERLGANALLAVSMACCKAGAADSGMPLYQYIGRLAKVTPNTMPVPMCNVINGGKHAGQ
                     ENSIQEHMIMPVGAVNFTEGIRMVAETYHHLQKMLKKKLGAGATLIGDEGGFAPSQIK
                     TVDERLELMTQAVQAAGCADKMAFTLDPAASEFFEDGVYTIGDKKMSGGELIDFYGDL
                     VGKYPIVSIEDGLAEDDWDSWVEMTKKLGRKIQIVGDDLFVTNTKRIEKGINIKSANS
                     VLIKLNQIGTVTETLNAIKMAQENNWTAVVSHRSGETEDNFIADLCVGTNAGQIKTGA
                     PARSDRNSKYNQLLRIEEELGSKATYPGRDFR"
     gene            5320..5553
                     /locus_tag="VFF28_02755"
     CDS             5320..5553
                     /locus_tag="VFF28_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44582.1"
                     /translation="MPDRYLIDTSIWVDLYEDRKGFCNEPLGDYALKLLSGIVAKGDI
                     VVLSDLLIRELESNYSMEQINGFFKLLKIASVK"
     gene            complement(5728..5988)
                     /locus_tag="VFF28_02760"
     CDS             complement(5728..5988)
                     /locus_tag="VFF28_02760"
                     /inference="COORDINATES: protein motif:HMM:NF036989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HZX44583.1"
                     /translation="MTTEMITVKMDDSFLKEIDDIVATQGYHNRTEFIRSALREKVEE
                     EKMKEAMLKLAHMKGSSNKKTTDDDLENLREKVFKELDKRVN"
     gene            complement(6118..6888)
                     /locus_tag="VFF28_02765"
     CDS             complement(6118..6888)
                     /locus_tag="VFF28_02765"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HZX44584.1"
                     /translation="MSTWKVEEFEKPNLKDPIFIEGLPGIGNVGKVVADFIVDELKAK
                     KIYEITSDAFPHSVLVTEDNLVELPKIELFYKKCNGQDFIFMAGDVQPIDESSSYEFS
                     EKVLDIVQGLGVSEIITLGGIGLSEIPKEPKVFCTGNNKEMIKKYADKKINTNLYGVV
                     GPIVGVTGLLLGLAGKRKINAIALLAETYGHPMYLGVKGAREVLKILNDKLNLGVDVD
                     KLEKEIAYIEKQILKRTKELSDVSKGVVKGTQEVNYIG"
     gene            complement(6885..7061)
                     /locus_tag="VFF28_02770"
     CDS             complement(6885..7061)
                     /locus_tag="VFF28_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004578218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-protein complex protein Nop10"
                     /protein_id="HZX44585.1"
                     /translation="MRHILKCQDCKTYTLKEKCPNCGKEVLRPQPPKFSPLDKYGDYR
                     RRAKHNSYEKADLI"
     gene            complement(7058..7828)
                     /locus_tag="VFF28_02775"
     CDS             complement(7058..7828)
                     /locus_tag="VFF28_02775"
                     /inference="COORDINATES: protein motif:HMM:NF003064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     alpha"
                     /protein_id="HZX44586.1"
                     /translation="MFLKKTGFPEEDELVLCTVTNIFHHGVFVDLDEYARSGMIHISE
                     VSPGRIRNIRDFVQQGKKIVCKVLRINQEKGYIDLSLRRVNERQKKEKINEIKQEQKA
                     EKIIEFAAKKHEIEPKKLYDEVAPKLLEKYGMLNPAFEDIVKGNLNLEKIGVDKKMAK
                     ALTELILERMKPTEVYVERELSLKSLAPDGVEAVRQALKQASLENITILYEGAGKYKV
                     KAKANEYKDAEILLDKSTGAAIESIEKSGGEGSVAKAE"
     gene            complement(7830..7907)
                     /locus_tag="VFF28_02780"
     CDS             complement(7830..7907)
                     /locus_tag="VFF28_02780"
                     /inference="COORDINATES: protein motif:HMM:NF013803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44587.1"
                     /translation="MCGKKIAEPTGGKAKVKARILEVLE"
BASE COUNT         2207 a         1764 c         1439 g         2502 t
ORIGIN      
        1 atcacgaagt tattgttgat actaacagcc atgacaagca gctttgggac aaggacaaaa
       61 accatatctc ggagctgtta aaagttattg caaaaaggat aacagagatg gagaaagtaa
      121 aatgtataaa atatgtcctt gccttcaaga atcacggaga aaaggcaggc acttcgatac
      181 tccattctca tgtccagcta gcttcagttg cgatgattcc gactgagatt aagacaaaga
      241 tagctgcagt aaacgagcac aaaagatgcc cttattgtga gattattaag atagaaagca
      301 aaagcaaaag aaagtgcttt gagaacaaag actttgtcgc atttgcacca tatgcttcaa
      361 gatttaatta tgagatatgg ttcttcccaa aaaggcatgt aacaaggatg gagaacctaa
      421 aagaaagcga gatttcaagc cttgcagaac tgatccataa aacattgaca aaactaaaaa
      481 gcatcaatgc ttcatacaat ctattcatcc aatattctcc aggcaaagaa gacctgcatt
      541 tccatataga gataacaccc aggatagcga catgggcagg ctttgaattt tcttcaggaa
      601 ttgttataaa ttcagtctct cctgaatcag cagcaggatt ctacagaagt tagataaggc
      661 tttttccatc catctctttt ggcttttcaa tgcccagtaa atttaacaca gttggagcga
      721 tatctccaag gcctcccttc ttaagcgcat gtttcttatc ctttgaaaca agcaccaacg
      781 gaacaggatc tattgtgtgg cttgtcttcc attccggcga ctggtcttcg atattcccat
      841 gatctgcaaa tatgagcatc gtgtagttct tatcaagccc ggctgtcact attttaccca
      901 cacattcatc aaccaccttc actgccttta agcatgcagg aacaactcct gtatgcccga
      961 caagatcgcc gtttacgata tttacgacaa taaatccata tttatccgtc tctatctcag
     1021 aaacaagccg ctcagtcact tcataagcgc tcatctcagg cttcaggtca taagtagcga
     1081 cctttggcga agggatcatc gccctatctt cgccgtcata aggtgtttct ctctgtccgt
     1141 tgaaaaagaa agttacatgg gcatattttt ctgtctcgct tatcctaagc tgcttgattc
     1201 catgcctgct gatgacctcc ccaagcaggt ttgcaagtga ctggtctaaa aaagcagcat
     1261 gggcgttcat gggcttgtaa tattctgtca ttgtcacata aaatacatcc aaaggccctc
     1321 tcttccaccc agcaaagtct ttttcaacta ttgcctgtgt cagctgcctt ggcctgtctg
     1381 tcctgaaatt aaagaagatt acactatctt tatctttgat gccattatat cctttcaatc
     1441 ttattggaac gatgaattca tctgtctccc tgtttgcata gcaccatcct actatcccaa
     1501 gaacatcgct gaattcacca ccctcgccaa gaacgatggt atcatacgct ttctttgtcc
     1561 tgtcccatct tttgtccctg tccatcccat agtatcttcc tgacaaggtg gttattttac
     1621 caaagccaag ttcttttagc ttttcaacaa gtttttggat atgcttcaag ccgtcgccag
     1681 gaggagaatc tcttccgtca gtgatgacat ggatataaac atcattgaaa ctctcttttt
     1741 tgcacagctc aagcaaggca taaagatgag cttcatgtga atgtacgcct tcgctttgta
     1801 aaagtcctat gagatgcaat ttgctcttgt tcttcttgca gttggaaata gctccaagaa
     1861 aagccttgtt cttataaaaa ctgccgtctg caatggagtt atttatcctt accatcggtt
     1921 ggaatatggt ccttcccgca ccgatggtca tgtgccctac ctcagaattg ccctgatatc
     1981 cctcaggaag cccaacagcc tctccatcag ccttgatcaa ggtattggga tattctttca
     2041 tcaaccggtc tgtgacaggc gttccagcct caaagatagc attgtcttta tgctcttttc
     2101 ggtatcccca tccatccctt ataataagaa ttactttgtt catttcatca atacctctac
     2161 gattattatc tatgttatct atatttcaat tttatttaaa aattattcgc tagtaatatc
     2221 aaatatatcc gccccgaatg ttcttaaaaa agggccattt cattctagtc taataaagaa
     2281 agcttttata tcaaaaagca atccctaaca tcagataaaa atgttgatga aatttgaccc
     2341 tggactgaca agacaggaga tttgggcaga gggtagtgaa cttgccacct tgctaggcta
     2401 cgatagggat gaacatggta aaagagttca ctcctggcca aaagaaagaa aaatccatgt
     2461 tgcaacacta gataatcctg ttctcgtcac agacagctac aaaatttgcc aatttcctag
     2521 cacgctaagg gatactgttt ttgctttgtt tgcatctcca actaaaacaa cagaatataa
     2581 aggcacaagc cttgagtttg agcaaaggag ataccctgga gtttggggtc cgtctatcga
     2641 caccctattg atgtgagagc gctagatcca agtgagctca cagcagtgca aactgcctgc
     2701 gagataggac ctggctcggg atatgtatca ttatacgtaa ggaacaatgc acctcactta
     2761 gagcaaattg acttggttga tctgagtgac agtgctatag cttgtgcaag agaagtgata
     2821 aaagacagaa gagcgactta tgcagtagga gatggaagag aattcttagt gggaaaaaag
     2881 tatggtttag tttactgcaa tcctccttac atcctaaggc caaaaagcat cgatgacaat
     2941 ccgtatgagg gaatttgtct tttagtctac cttgtaaccc atgctgatga attcctaaat
     3001 aaaggcggca gattagtcac taatgtatca agcctggcag agccagtcac aactccgttg
     3061 tttgcagata caggtattag aacgattgac cagatgcatg ttcccttaaa agttttcaac
     3121 gttttgaaca acccggagtg gatggattat ctgttgagtg aaaaagggct cagaaaagag
     3181 tcaagagacg gttatgatta ctggcatact atttctattg ctgaagtaag aaaaagatag
     3241 attaaagtag aaatcccatc attatctaat tcaagctaaa aacaagccca aacgtagcca
     3301 ttgtttcatc tggcttaccc tgttgtcaat gcccgaaatt cagatgcata actttgtgca
     3361 gtaaaggcta gggatcctat agcaaaagca gcaaccgcaa gtatcctctt aatccttggc
     3421 ttataagcca taatttagag gattggtgga tcatttataa gtattttgag aaaaaatagt
     3481 taaataaaaa aaacttacct gaaatctctt ccagggtatg ttgctttgct tccaagctct
     3541 tcttcaatcc taagcagctg gttatacttg gagttcctat cgcttcttgc aggagcgcct
     3601 gtctttatct ggcctgcgtt tgttccgaca cagagatcag cgataaagtt atcctctgtc
     3661 tctccgcttc tatgcgagac aacagcagtc cagttgttct cctgtgccat ctttatggca
     3721 ttcaaagtct ctgtgacagt tcctatctgg tttagcttga taagcacaga gtttgcggat
     3781 tttatgttta tccccttctc tatcctcttg gtgtttgtga caaacaggtc gtctccgact
     3841 atctgtatct ttcttccaag cttctttgtc atctcaaccc atgaatccca atcgtcttcg
     3901 gcaagtccgt cttctatgct tactataggg tatttgccca caagatctcc atagaagtcg
     3961 ataagctctc cgccgctcat cttcttgtcg cctattgtgt aaacgccatc ttcaaagaat
     4021 tcagatgctg caggatcaag tgtaaatgcc atcttatcgg cgcagcctgc agcttggact
     4081 gcttgtgtca taagttcaag cctttcatcg acagtcttta tctggcttgg tgcaaatcct
     4141 ccttcgtccc ctatcagggt agcgccggct ccaagtttct tctttaacat cttctgaagg
     4201 tgatgatagg tctctgcaac catccttatc ccttctgtaa aattgacagc gccaacaggc
     4261 attatcatat gctcctggat ggagttctcc tggccagcat gtttgccgcc gtttatgaca
     4321 ttgcacattg gaacaggcat tgtgtttgga gtgacttttg caagccttcc gatatattga
     4381 taaagcggca taccggaatc agcagcgccg gctttgcagc atgccataga taccgcaagc
     4441 aaagcatttg ccccaagcct ttctttgttt tcagtgctat caagctctat aaggtcatca
     4501 tcgatatcta tctggtcgct tgcctctatt cccaccaagg tttcagctat ctcatggttg
     4561 acgttcctta cagcgttatg gacgcctttt ccaagaaatc ttttattatc cccatctctc
     4621 atctccagtg cttcgtgaac accagcagag gcgcctgatg gaacgcttgc cctaaacaga
     4681 cctttttcag ttacaatatc acattcaaca gttggatttc cccttgaatc cagtatctct
     4741 ctagctttga cgctttttat cttgctcata ttaacacctc gaaccgttta ctcgcagaat
     4801 gaggagttag tataaaagaa ttacgttttg ctggtcacta aatatataat ttatataaaa
     4861 agaggagttg gagtataatg gctaatagaa gaggttgtag actgatgtgt tcgcttgatc
     4921 atttttttaa cccctttatg gtatagggca caaagggtga ttataacacg tttaacgacg
     4981 gaaaaaaaga aaatgtttaa atagctaagt taaatctttg ctaagtatgc cttgtgctaa
     5041 tcatccaaat gcatttactt acttccttgc tttgcttttg ctgattggac tttaacaaaa
     5101 tataaagtct acacaggtaa taattttcta agaatattaa ctggattttt gttgggtata
     5161 agcggtgcag gattcatcta tctgttttta tggaacccat ttactagtga ggtctacata
     5221 gctctggcaa tttatttgct gattatggtt attagtttac attctgtaag taagggctag
     5281 tccataaatt attaaaacct cgaccatttg ctatcaaata tgcctgatag atatcttatt
     5341 gatacatcaa tttgggttga cttatacgaa gatagaaaag gtttctgtaa tgagccatta
     5401 ggtgactatg ccctaaaatt attgtcaggg attgttgcaa aaggggatat cgttgtcctt
     5461 tccgacctgt tgataaggga gcttgagtcc aattattcaa tggagcagat aaatggattt
     5521 tttaagttat tgaaaattgc atcagtaaaa taatatcaac aatagagcaa agggacgagg
     5581 caaaaaggat agcgcaagaa aggaatgtcc caagaggaga tgctttgcat gcaatattag
     5641 caagagataa taaacttatt ttgattactc gagataaaca ctttaggcaa ttagaagata
     5701 tcgccaaatt ttataggcca gaagacgtta gttaactcgt ttatccaatt ctttaaagac
     5761 tttttccctt aggttctcaa gatcatcgtc agtagttttt ttatttgagg atcctttcat
     5821 atgagcgagt ttcagcatcg cttccttcat tttttcctct tctacctttt ctctaagagc
     5881 acttctgatg aattccgttc tgttgtgata accctgtgta gctacaatat catctatttc
     5941 ttttaaaaac gaatcatcca tcttcactgt gatcatttca gtcgtcatat agccattgta
     6001 tatacattag atatataaaa ctttgcttct taaatctcat agactaaaaa gcaattcaaa
     6061 gaaatttaat ggcccatatg tttagacgcc gaacaccttg aaactctcta aaataaccta
     6121 cccgatataa ttaacttcct gcgtcccttt gacgactccc ttagaaacat cagatagctc
     6181 ctttgtcctt ttcaatatct gcttctctat gtaagctatc tctttttcaa gcttatcaac
     6241 atctacgccc aggtttaact tgtcattaag tatcttgaga acctctcttg cacctttcac
     6301 accaagatac atcggatggc cataggtttc agcaagtaaa gcgatagcat ttatctttct
     6361 tttaccagca agacccagca gtagtccagt tacaccgaca attgggccga caaccccgta
     6421 aagattggtg tttattttct tatccgcata tttcttgatc atctctttgt tattgccggt
     6481 gcaaaatacc ttcggctctt tagggatctc agaaagccct attccaccaa gcgtgattat
     6541 ctcagaaact ccaagcccct ggacaatatc taaaaccttt tcagagaatt cataagagct
     6601 tgactcatct atcggctgca catctcctgc catgaaaata aaatcctgtc cattgcattt
     6661 tttataaaac agctcgatct ttggcagttc gacaaggtta tcctcggtaa ctaaaacaga
     6721 atgggggaaa gcatcagagg ttatctcata tatttttttg gcctttagtt catcgacgat
     6781 aaagtcagcg acgactttgc caacattgcc tatccctggc aatccttcta tgaagatagg
     6841 atccttaaga tttggttttt caaactcctc tactttccat gtgctcatat cagatcagct
     6901 ttttcgtatg aattatgttt tgcccttcgc ctataatccc catatttatc aagaggcgaa
     6961 aactttggcg gttgcggcct taaaacttct tttccgcaat tagggcattt ttccttaagg
     7021 gtatatgtct tacaatcctg gcacttcagt atatgcctca ttctgccttg gcaacagagc
     7081 cttctccgcc gctcttttct attgactcga ttgccgcccc tgttgactta tcaagaagta
     7141 tctcagcatc tttatattcg tttgcttttg ccttgacctt atattttcct gccccttcat
     7201 atagtatcgt aatgttctca aggctagcct gttttagggc ttgcctaaca gcttcaacac
     7261 catctggtgc aaggctctta agagaaagct ctctctcgac atagacttct gtaggtttca
     7321 tcctctctaa gataagctct gtcaaggctt tggccatctt tttatcgacg cctatttttt
     7381 caagattaag atttcctttg actatatctt caaacgcagg atttagcatg ccatatttct
     7441 caagaagttt tggggcgacc tcatcataca gcttttttgg ttctatctca tgcttttttg
     7501 ccgcaaactc gatgattttc tcagccttct gctcctgctt tatctcgttt atcttttcct
     7561 ttttctggcg ctcatttacc cttctaaggc ttaggtcgat ataacccttc tcttgattga
     7621 ttctaagaac cttgcagact atcttcttac cctgctgtac aaaatccctt atgttcctta
     7681 tccttcccgg agaaacctcc gagatatgga tcattccgct tctggcatac tcatctaaat
     7741 cgacaaaaac accatggtgg aatatattgg taacagtaca tagaactaat tcatcttctt
     7801 ctggaaaacc tgtctttttt aaaaacattt tactctaaaa cttcaagtat gcgcgccttt
     7861 acctttgctt taccgccggt aggctctgca atctttttcc cgcacacaag gc
//
LOCUS       DASRUK010000057         3781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_714427, whole genome shotgun sequence.
ACCESSION   DASRUK010000057 DASRUK010000000
VERSION     DASRUK010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714427"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="VFF28_02785"
     CDS             complement(<1..911)
                     /locus_tag="VFF28_02785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA surveillance protein pelota"
                     /protein_id="HZX44588.1"
                     /translation="MKQLSFDIKKGEAKLRVDNEDDLWYLSQIIDIGDTINGKTMRKI
                     KIGEESERKQAIVKKPVFISIAAEKIEYSNNSLRVLGTITEAPEDIQKGEHHTFAVEE
                     GTTFTLIKERWLKYQLDRLKEASKEAQSRILIVVMDREEASFAIMKKYGYELLSSIKG
                     NVRKKAIEERHSSNFYEEIISKMEEYVTRLGIVKIILASPAFWKEDLMKELKDVSLKK
                     KIILATCYGTGKNGIDEVLKRPEVRSALQEDRMSKEVNLVEQLLAEVSKGAFAVYGLK
                     ETESAVNAGAVKNLLVTDGYIQKARAE"
     gene            1004..2170
                     /locus_tag="VFF28_02790"
     CDS             1004..2170
                     /locus_tag="VFF28_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44589.1"
                     /translation="MKKAQGGLNAAILVAVIAAMIIIYILFLPAEDREKLLEEKSDYA
                     RDSTAKDDTLLLSENIGRLDPIGKVNDKTIANVYIFETTNSKVLDTINPIYVRNGWFD
                     KNVKIVQFKVGDLQNTDNVFLAFTAPKRKGVLSIQLNGESIYEYDINTANIEPIKLKK
                     SSLQEDNELLFSVSGVGAAFWKTNEYSLENVKIYGDITDLSRQQSQNVFSLSDTEYSN
                     VEKVTLKFIPYCTTNVGVLDVLVNNRNIFSAVPVCNDRVVQDIPLSALTSGQNNVVFK
                     TNKGSYSVEQIILSFKQKNVPESVMYFEVNSSTFKDIDARKYDTYLTIDFVDDSDTKR
                     LDLNINDHLIRVDQEKKVFEREVGAWIEDGNNFIKITPKTTLEIVELNVELRKR"
     gene            2177..>3781
                     /locus_tag="VFF28_02795"
     CDS             2177..>3781
                     /locus_tag="VFF28_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44590.1"
                     /translation="MNLNEIRARIAEMKREAPPLGRESIPFYKELTKLKEDEQRLNQL
                     EAGKTAEIQRFRQRAAELDELIEKHPAMAQMLKNNRAFVMGQIEQRSAPANSDIEDIR
                     RRTEERMIALVEAAKKPHPNLFPKDEIPDDPRGRYPGKSQKKKDAPEETPPEEIPDEQ
                     ETEQEDKPQTPRSRRGRTNRGYATHLQSQSTPGRSVTGSPPNNITNVVVNTAKESASL
                     TLWLLLIVVLYAADWALGFAGINYQVLLEAFSAGGLELLLRLILPIHIVIIFTILLAL
                     KYAKTRGIIVFLVIVFVPLIIINMFTTDYKVLGISLSVASLITSLIVLMISLDPEDRR
                     DFFSWVLLIMVVTTIISLGGLVRGFHRLVIAGILWAAFIKLPEDEKFTRANYIIVALL
                     VIDFFIFGILQSFGPLKGNSILANRFIIPVWFLFVLFYGLKHKGKTNLLDKVLVGFII
                     FLYLMAFVDGYGGWTTIQGQLQTNPQDITQAKGFFAAAWKKMTQFPGKIVEEYNKGMN
                     AATGGYYQGKVEENQDPRNNLGVHIEK"
BASE COUNT         1134 a          758 c          795 g         1094 t
ORIGIN      
        1 ctttcagccc ttgctttttg tatatatcca tctgtgacca acaggttttt tactgctcca
       61 gcgtttactg cgctttcggt ttcctttaga ccatagactg caaaagctcc ttttgagacc
      121 tctgcaagga gctgctctac aagattgact tcttttgaca tccggtcttc ctggagcgct
      181 gaccttacct cgggcctttt taacacctca tctatgccgt tttttcctgt accgtagcat
      241 gttgcaagga tgatcttctt ttttagacta acatccttta attctttcat caagtcttct
      301 ttccaaaagg cagggcttgc aagaatgatc ttaacgatgc ctaatcgggt tacatattct
      361 tccatcttgc tgatgatctc ttcatagaag tttgaagaat ggcgctcttc tatcgctttt
      421 ttcctgacat ttccttttat tgaagacagc agctcatagc cgtatttttt catgatggca
      481 aaactagctt cctccctatc catcaccact atcaggatcc tggactgcgc ctcttttgag
      541 gcttccttaa gcctgtcaag ctgatactta agccatcttt ccttgatgag ggtaaaggtt
      601 gtaccttctt ctacagcaaa ggtgtgatgc tcaccttttt gaatgtcttc tggcgcttct
      661 gtaatggtac ctagaaccct taatgagtta ttgctatatt caatcttttc agctgcaatc
      721 gagatgaaga cgggtttttt tacaatggct tgctttcttt ccgattcctc acctatcttt
      781 atctttctca tggttttgcc attgatagtg tctccgatgt ctattatctg gctaaggtac
      841 caaaggtcat cttcattatc gacccttagc tttgcttcac ctttttttat gtcaaaagaa
      901 agctgtttca tgtcaatgcc taaaataaga agctttatat ataaatctat tcgtaaaacg
      961 agtaaagtta gtcaaggctc taaattaaaa aaggtgattt gaaatgaaga aagcccaagg
     1021 cggcctaaat gctgctatct tagtcgcggt tattgctgca atgatcatca tctatatcct
     1081 ttttttacct gctgaggaca gggaaaaact tcttgaagaa aagagtgatt atgcaagaga
     1141 ttcaactgcg aaagatgata ccctgctgtt atcagagaat atcggaaggc ttgatcctat
     1201 cggtaaggta aatgacaaaa cgatagcaaa cgtatacata tttgaaacga ccaattcaaa
     1261 agtccttgac acaattaatc ctatctatgt gagaaacgga tggtttgaca agaatgtaaa
     1321 gatagtgcag tttaaggtag gcgacctgca aaatactgac aatgttttct tggcatttac
     1381 ggcaccaaaa agaaaggggg tgctttctat ccagctaaat ggcgagtcaa tctacgagta
     1441 tgatataaat actgccaaca tcgagcctat caaactcaag aagagctcgc ttcaggaaga
     1501 caacgagcta ctttttagcg tctctggagt tggagctgct ttttggaaaa caaatgaata
     1561 ctcgcttgag aatgtaaaaa tctacggaga tataactgac ctctcaagac agcagagcca
     1621 gaatgtattc agccttagcg acacggagta tagcaatgtc gaaaaagtta cgttgaagtt
     1681 cataccctat tgcactacaa atgtgggggt gcttgatgtc cttgtcaaca acaggaatat
     1741 tttttctgca gtccctgtat gtaatgacag ggtagtgcag gatatccccc taagtgcgct
     1801 tacatcaggg cagaacaatg tagtgttcaa gacaaacaaa gggagctatt ctgtagagca
     1861 gataatactt agcttcaagc agaagaacgt ccctgagtcg gttatgtatt ttgaggtaaa
     1921 ctcttcaacc tttaaggata tcgatgcaag aaagtatgat acctatctca caatagattt
     1981 tgtcgatgac agcgacacaa aaaggcttga ccttaacata aacgaccatc ttataagggt
     2041 tgaccaggag aagaaagtgt ttgagagaga agtaggtgct tggatagaag acggcaacaa
     2101 cttcatcaag ataacgccaa agacgacgct tgagattgtt gaactcaatg tggaattaag
     2161 gaagagatag attcttatga atttaaatga gataagggcg agaattgccg agatgaaaag
     2221 agaggctcct cctttgggcc gagaaagcat ccctttttat aaagagttga ccaagcttaa
     2281 ggaggatgaa caacgattaa atcagcttga agcaggcaaa acagctgaaa tacaaagatt
     2341 tagacaaagg gcagcagagt tagatgaatt gattgagaaa caccctgcaa tggcccaaat
     2401 gctcaaaaat aacagggcat tcgtcatggg gcaaattgaa cagagaagtg ccccagctaa
     2461 ctcagatata gaggatatac gacgaaggac ggaagaaagg atgatagcac ttgtggaggc
     2521 tgctaaaaaa ccacatccaa atctttttcc aaaagatgaa atccctgatg atcccagagg
     2581 acgatatcca ggcaagagcc agaaaaaaaa agatgctcct gaagaaacac cgccagaaga
     2641 aatccctgac gaacaggaga ctgagcagga ggataaacct caaacaccaa ggagtcgaag
     2701 aggtcgaact aacaggggct atgcaaccca tttacaatct caatccacgc caggtcgatc
     2761 tgtaactggt tcaccaccaa ataatattac taatgttgtc gtcaatactg ccaaagaatc
     2821 tgcatccttg acattgtggc tgctattaat cgtcgtatta tatgctgctg actgggccct
     2881 gggttttgct gggataaact atcaagtgct cctggaggca ttcagtgcgg gcggccttga
     2941 gctcctgcta aggttaatac tccctatcca tatagtaatc atattcacta ttcttcttgc
     3001 attaaagtat gcaaaaacac gaggaataat tgttttttta gtcattgtct ttgtgcctct
     3061 aatcataata aatatgttta caacagatta caaagtatta ggtatctcat tatctgtggc
     3121 ctcattgatc acctcgctga ttgtcttaat gataagccta gacccggagg acaggcgaga
     3181 tttcttttcc tgggttttac ttataatggt agtaaccaca atcatctccc ttggcggtct
     3241 tgtcaggggt tttcatagat tagttatcgc tggaattttg tgggcagcat ttattaaact
     3301 gcctgaagac gaaaagttta cgcgtgcaaa ttatataatt gttgcattac ttgttatcga
     3361 ttttttcatt tttggcatac tgcaatcttt tggcccacta aaaggaaata gtatcttagc
     3421 aaacaggttt atcattccag tctggttctt gtttgtcttg ttttatggtc ttaaacataa
     3481 gggtaaaacc aacctcctgg ataaggtatt ggtcgggttt atcattttct tgtatctaat
     3541 ggcatttgtc gatgggtatg gcggttggac tacaatccaa gggcagttgc aaaccaatcc
     3601 ccaggacata actcaagcaa agggcttttt tgcagctgcc tggaaaaaga tgactcaatt
     3661 tccaggaaaa atcgtcgagg aatacaacaa ggggatgaac gctgccacgg gtggatatta
     3721 ccagggcaag gtagaggaaa accaggatcc gcggaacaac cttggggtcc atatagaaaa
     3781 g
//
LOCUS       DASRUK010000058         4353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1055395, whole genome shotgun sequence.
ACCESSION   DASRUK010000058 DASRUK010000000
VERSION     DASRUK010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055395"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFF28_02800"
     CDS             <1..262
                     /locus_tag="VFF28_02800"
                     /inference="COORDINATES: protein motif:HMM:NF013647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HZX44591.1"
                     /translation="YVRLMAIGVASVGLALVVNEFAVGFFQKGGLFIAAGVLILVVGH
                     IINIGLGLLGCFLHTLRLHYVELFSKFYKGGGVAFKPFGEQN"
     gene            284..526
                     /locus_tag="VFF28_02805"
     CDS             284..526
                     /locus_tag="VFF28_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HZX44592.1"
                     /translation="MAVEAIANTSTGLIALSAGIAISVSALAAAWAEKTIGTAAIGAM
                     AEKEELFGKGLILTVIPETIVIFGLIVAIMIIGMAG"
     gene            529..1041
                     /locus_tag="VFF28_02810"
     CDS             529..1041
                     /locus_tag="VFF28_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44593.1"
                     /translation="MGLEEVKNEILEKSKAEIKKIESHKERELNLIAENTEKSIAQRQ
                     KRLDEEIEHKIEAMKQRNKAEMIAEKRDLLLRIKNEQIEKAMAEAKKKLKKQEEKYVQ
                     LLLEKAKKQISVGKVYAKHEIPGIKTEIKDIDGVIVESKDGKFLLDYSFATILAGIKE
                     KRLKEISEAL"
     gene            1038..2012
                     /locus_tag="VFF28_02815"
     CDS             1038..2012
                     /locus_tag="VFF28_02815"
                     /inference="COORDINATES: protein motif:HMM:NF014094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HZX44594.1"
                     /translation="MRKKIRMEESPYTFVRAKVMKSLLIPRSEYKKLLGMSISEIARY
                     LSETQYRDDINELGVDFSGLALIEQALNRNFSRMVEKLRRISPHSYNQVIDAYLLKYD
                     IENIKTLLRAKQAGTSEDDAMKLMMPAGKLSSKQLRLIFKKNQAEILEAFEGTDAKSL
                     TEVETNLDHYYFSSMLDFADKVPRQGKLLKEFILNNVHTANVLTFLRLKRLGMENKQI
                     EKHIFFTPKYSFFYRHLLQAKSGEESQKMIRQHFPINSFDSLTDVEISLQKYLVSHSN
                     FEHQNPLSVYIILEFLMAKEIELKNLKRIISAKHLGMDMSLVEPMVVA"
     gene            2009..2314
                     /locus_tag="VFF28_02820"
     CDS             2009..2314
                     /locus_tag="VFF28_02820"
                     /inference="COORDINATES: protein motif:HMM:NF014092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HZX44595.1"
                     /translation="MKQIACLGSEAFILGFQMAGIKAITYDKNRVYDQLVSLKDDPNM
                     GIVIVEEALLAQLEGDKQADIMDCVSPVFFPLSAEESFGNIRELIIKSIGVDLMKGE"
     gene            2316..4037
                     /locus_tag="VFF28_02825"
                     /pseudo
     CDS             2316..4037
                     /locus_tag="VFF28_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328188.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
     gene            4043..>4353
                     /locus_tag="VFF28_02830"
     CDS             4043..>4353
                     /locus_tag="VFF28_02830"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107919.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the B subunit is part of the
                     catalytic core of the ATP synthase complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HZX44596.1"
                     /translation="MKEYKTINKVAGPLIFVEKTEPIGYADIVQIKLDSGEIKNGQVL
                     DTSEDLVVVQIFEGTSGIGKEAKVKFLGETLKLSLSEDMLGRVFSGRGAPKDGKAPII
                     P"
BASE COUNT         1409 a          820 c         1100 g         1024 t
ORIGIN      
        1 ctatgtcagg ctgatggcaa taggagtcgc atctgtcggc cttgctttgg tagtcaatga
       61 atttgctgtc ggtttttttc aaaaaggagg attgtttatt gcagcaggag tgcttatcct
      121 tgtagtaggg catatcataa acatcgggct tggccttctg ggctgctttc ttcacactct
      181 taggcttcat tatgttgaat tattcagcaa gttttacaaa ggaggagggg tagccttcaa
      241 accctttgga gaacagaatt aaacagaacg gaggaatgaa aaaatggctg tagaagcaat
      301 agcaaataca agcactggcc tgatagcatt gagcgcagga atagcgataa gcgtgagcgc
      361 acttgcagct gcatgggcag aaaagacaat aggtactgct gcgataggag cgatggcaga
      421 aaaagaagag ctatttggga aagggcttat ccttacagtc atccctgaga caatagttat
      481 cttcggtctt atagtggcta taatgataat cggcatggct ggttaagaat ggggctggaa
      541 gaagtcaaaa acgaaatatt ggagaaatcc aaggcagaga taaagaagat agaatcgcat
      601 aaggaaagag agctcaatct gattgcagag aatactgaga agtctattgc gcaaaggcaa
      661 aagagactgg atgaagagat cgagcataag attgaggcta tgaagcagag aaataaagca
      721 gagatgatag cggaaaaaag agaccttctg ctcagaatta agaacgagca gattgaaaaa
      781 gccatggcag aggcaaaaaa gaagcttaag aagcaggaag aaaagtatgt ccaactgctc
      841 cttgagaagg caaagaagca gataagcgtc ggcaaggttt atgcaaaaca tgagataccc
      901 ggcatcaaga cagagataaa agacatcgat ggagtgatag tggaaagcaa agatggcaaa
      961 ttccttctgg actacagctt tgccactatc cttgcaggga taaaggaaaa aaggctaaag
     1021 gagatatctg aagcgctatg agaaaaaaaa tccggatgga agagtcacct tatacatttg
     1081 tcagggcaaa ggtgatgaaa tcgcttctta tcccgaggtc tgaatataag aaactgcttg
     1141 ggatgagcat ctctgagata gccaggtatc taagtgagac tcaatatcgc gatgatataa
     1201 acgagcttgg agtggacttc tcagggcttg ctttgataga acaggcgcta aaccggaatt
     1261 ttagccgcat ggtggaaaaa ctaagaagga tatctcccca ttcatacaac caggtgatag
     1321 acgcatatct gctaaagtat gacatagaaa acataaagac cctgcttagg gcaaagcagg
     1381 ctggaacaag tgaagatgat gccatgaagc taatgatgcc tgcaggcaag ctgtcctcaa
     1441 agcagctgcg gcttatcttc aaaaaaaacc aggcagaaat actagaagcg tttgaaggga
     1501 ccgatgccaa aagcctgact gaggtagaga caaatcttga ccactattat tttagctcta
     1561 tgctcgattt tgccgataaa gtcccaagac aaggcaaact tttgaaggaa tttatcctta
     1621 acaatgtcca tacagccaat gttctgacat ttcttcggct aaaaaggctg ggcatggaaa
     1681 acaagcagat agaaaaacat atcttcttta ctccaaagta cagtttcttt tatagacatc
     1741 ttctccaggc aaaaagcggg gaagaaagtc aaaagatgat aaggcagcat tttcctatca
     1801 acagttttga ttctctgacc gatgttgaaa tatcgctcca gaaatacctc gtatcacact
     1861 caaattttga acatcagaac cctttgtctg tgtatataat ccttgaattc ctaatggcaa
     1921 aagagataga actgaagaac ctcaagagga taataagcgc caagcacctt ggcatggaca
     1981 tgtctttagt cgaaccgatg gtggtagcat gaagcagata gcatgccttg gcagtgaagc
     2041 tttcatcctt ggatttcaga tggcagggat aaaggcaata acctatgata aaaaccgggt
     2101 ttatgaccaa ttagtctcat tgaaggatga tccaaatatg ggcattgtca ttgttgaaga
     2161 agctttgctt gctcaactcg agggcgacaa gcaggcagat ataatggact gtgtatcgcc
     2221 tgtctttttt ccgctttcag ctgaggaaag ctttggaaat ataagggaac ttattataaa
     2281 gtcgataggc gtggacttga tgaagggtga ataaaatggg atttttacac aggatagcag
     2341 ggccagtagt ggttgcaaag gatataaaag caaatatgta tgatcttgtc aaggttggaa
     2401 acgagaagct tatgggagag gtcatacaga tagatggaga caacaccatc attcaggtct
     2461 atgaagatac ttcaggacta aagccaggag agcctgttgt caatacaaac aagcccctta
     2521 ctgtggagct tgggccaggc cttcttacca gcatctatga tggcatccaa aggccgcttt
     2581 cagtgctgaa acagaagcaa ggagattata tcttcagagg tcttgaggca ccgggacttg
     2641 accatgataa aaaatgggat tttaaagctg aagccaaatc cggagataag atctctggag
     2701 gaagcatact tggaacagtt gaagaaacct aagggatagt ccatcgagtg cttgtgcctc
     2761 cgggagtaag cggcaagata aaacaggtga aatctggctc atacactgtt gaggagactg
     2821 tctgcacgct agatgacggg acaaagatca agctaaagca agaatggcct gtaagaaagc
     2881 caagaccaag cctaaaaaag ttgaatccca tcgtcccgct tatcacaggg cagaggatat
     2941 ttgatgctct ttttccgctt gcaaagggag gagtcgctgc aattcctggc ccatttggcg
     3001 caggaaagac tgttacccag cagacccttg ccaagtggtg cgatgcagac atcatcatct
     3061 atgtaggctg cggtgaaaga ggcaacgaga tgactgaagt gcttaaggag tttcctgagc
     3121 ttgtcgatcc aaaaagcgga aggcctctta tgcacaggac cgtgcttatc gccaatacat
     3181 ccaatatgcc tgtcgctgca agggaagcat ctgtctatac aggagttact cttgctgagt
     3241 attataggga catggggtat tctgtcgcta tgatggctga ctctacatcc agatgggcag
     3301 aggctatgag agagatctca tccaggcttg aagagatgcc tggcgaagaa ggatatcctg
     3361 catatcttgc tacaaggctt gcgcagttct atgaaagggc tggaagagta gtggctttgt
     3421 gcaacaaaga aggctctgtg accataatcg gagcagtaag ccctccaggc ggtgacttct
     3481 cagagccggt gacacaaaat acattgaggg taacaaaatg tttttgggcg cttgatgcaa
     3541 agcttgcgca aagaaggcat tttccatcta taaattggct gacttcttat tcactatacc
     3601 ttgacagcct gcagccgtgg ttttccgagc atgtatcaac cggatttaga aaccttgtgt
     3661 ctgagtttat gagcatactt tctgaagagg agaagcttat gcagatagtg cagcttgtgg
     3721 gttcagatgc gctgcctgaa aaagagcagc ttactcttga gacagcaagg atgataaggg
     3781 agtttttcct gcagcaaaac gcattccatc ctatcgacac attttgcgac ctcaagaaga
     3841 gcgagaggat aatgcagact atcctaaaat ataggagcct tggaatggat gcacttgctt
     3901 ctggtgtcaa ggcgcagaag atactgcaga taaaatccaa gaattcaatc gtcaattcaa
     3961 agttcgaaaa agactatgaa actgtgctaa atactgcggc caaagaaatg gaaaaagaga
     4021 tcgcaggtct aaggtgaatg caatgaaaga atacaaaaca atcaacaaag tcgccggccc
     4081 gttaatcttt gtggaaaaaa cagagcctat aggctatgcg gacatcgtgc agatcaagct
     4141 ggactcaggc gagataaaga acggccaggt gcttgacact tcagaagacc ttgtggtagt
     4201 gcagatattt gagggaacat caggcatagg caaggaagca aaggtgaaat ttcttggaga
     4261 gacacttaag ctctcgctgt cagaagacat gctgggcaga gtattttctg gaaggggagc
     4321 gccaaaagat ggcaaggctc caataatccc tga
//
LOCUS       DASRUK010000059         8687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2388268, whole genome shotgun sequence.
ACCESSION   DASRUK010000059 DASRUK010000000
VERSION     DASRUK010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 8687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8687
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2388268"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VFF28_02835"
     CDS             complement(<1..515)
                     /locus_tag="VFF28_02835"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HZX44597.1"
                     /translation="MNVLLVYPEYPKTFWSFKYVLSYVLKKAAYPPLGLLTIASMLPK
                     EWKKRLIDCNVKKLAKSDIAWADMVFVSAMIAQKSSVVELTKIFRTEGKLVVAGGPLF
                     TSSVEEFENIDHFVLNEGEVTLPLFLEDLKLGCPKKIYQSDVRPDVTKTAIPMWSLID
                     MRKYHSMLIQFS"
     gene            608..1054
                     /locus_tag="VFF28_02840"
     CDS             608..1054
                     /locus_tag="VFF28_02840"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HZX44598.1"
                     /translation="MEIETLAKEARNSIGEFCMEECKSYCCRKGYLSLSKKEAALLSD
                     NRIDEWVKKRLLHKLGKKYSFFLGSEQFPCPKLKDFKCTVHKDPNRPKACQNYPIFIK
                     GNKIRLSSRCLAVNADKLYPYISQFLKLGYKIEHSYSLEDADIYEK"
     gene            complement(1055..4279)
                     /gene="carB"
                     /locus_tag="VFF28_02845"
     CDS             complement(1055..4279)
                     /gene="carB"
                     /locus_tag="VFF28_02845"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HZX44599.1"
                     /translation="MPKRTDIKKILVIGSGPIVIGQAAEFDYSGTQACKALREEGYKV
                     VLINSNPATIMTDPDMADAIYIEPLTVEVICQIIHKEKPDGLLPTMGGQTGLNLAVAV
                     AKTGILSECNVEVLGTSIASIEEGEDRELFKNLMIKIGEPVPRSKTVTKVSEAVSFVK
                     DIGLPVVIRPAFTLGGTGGGIVYTDEELREITQHGLNLSPINQVLVEEAILGVNGWGE
                     FEMEVMRDKNDNCIIVCPMENFDPMGIHTGESIVTAPTQTLNDHDFQTLRTSAIKIIR
                     ALKVEGGCNIQFGLNYNTGEYRIIEVNPRVSRSSALASKATGYPIARIAAKIAVGMTL
                     DEIPNDVTKETKACFEPTIDYVVVKIPRWPFDKFATADKRIGTQMKSTGETMAIGRTF
                     EEALQKALRSLEVGRFGLGYDPKDKICTDKNEIIQNLKVATHKRLMYIRDGLNIGMSP
                     EEISKLTGINQWFLEKVKNVYEMGKEIKLEKESIFLAKRNGFSDVQIAKICGRKEEEI
                     RNFRIENKIIPTYKMVDTCAAEFKAYTPYLYSTYETEDEAMPSANDKKVIIVGGGPIR
                     IGQGIEFDYACVHSVFALREMGYEALIINNNPETVSTDFDTSDKLYFEPLTYEDVLNI
                     IERENPMGVILQFGGQTPINMALRLDKAGVKILGTQPEAIDIAENRNKFGKITDELKI
                     PSAEWGTAFSYDEALAVARRIGYPVLVRPSYVLGGRAMQIVDDDEELENYMIEAVNVS
                     PEHPILIDKYLDNAVELDVDALCDGKDVFVAAIMEHIEEAGIHSGDSACVIPPQNLSK
                     ELKERVIDYTTRLALSLKTVGLINIQMAVKDDVVYILEANPRASRTVPFVSKAVGLPL
                     AKMATRIMLGKSLKDLGLIGYREPKFVSVKEVVFPFLKLTGVIPELGPEMKSTGEVMG
                     MDSNFAKAFFKAEAASGTTLPSEGNILITIGKDEDKIAAVPIAKQLSSMGFKLFGTRS
                     TAECFNSNGIACETVPKIRENPLVLEMMKNKELQLVINIHRGTHPKSDSATIKRQCVE
                     LDIPYITTMTSLKPCLAAIKAQAKQKITVKSLDEYYKTEAIVTV"
     gene            complement(4487..5599)
                     /gene="carA"
                     /locus_tag="VFF28_02850"
     CDS             complement(4487..5599)
                     /gene="carA"
                     /locus_tag="VFF28_02850"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011031993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HZX44600.1"
                     /translation="MGANTSKSSGRAMNAVLALKDGTVFEGIGFGAESEKAGEVVFNT
                     SLVGYEESLTDPSYKGQILTLTYPLIGNYGVRNDNFESDFIQVEGFVVREHEDFFSHR
                     NAVKGVSQFLKEYSIPGISGIDTRMLTKKIREYGVINGALVTSKGDINTESVVKKAQK
                     LESYSSFDLLSKVSVKKPEFIDAGGEKSIALIDTGVKISIIRNFIKRKVNVYRMPFNT
                     KADEIRGLNVNGLFLANGPGDPEMAKDAINVVKEMQSEMPITGICLGNQIVGLALGGK
                     TYKLKFGHRGTNQPVKDIAQNKVFITSQNHGFAVDADSLDGTGLTVSHINLNDGSVEG
                     MRHERLPIMAWQFHPEAHCGPWDTDYLFDIMVKNLK"
     gene            complement(5750..6571)
                     /locus_tag="VFF28_02855"
     CDS             complement(5750..6571)
                     /locus_tag="VFF28_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44601.1"
                     /translation="MKDLTIVYMKDMSRFTPHFRHDPPYDASLLLQAYEKLPLEGLMP
                     SEVGFISVHPLEKKNAQDLLSVIESPTTTYTPLALIEAMESAKAGDDILSLQSAYRRL
                     SYLIISGWIHLDPEDIGILPDAITEVFTLDRYRESIASYISQNPSRVGEFAESIRMLL
                     DLPKSSDFQGIADLFRARLALNKVSPDYPLCYVPPSTAIPIIWASSPGIIGCAAGAGA
                     KLSSDQDISYRNFNVIDNLIEHLLSYGRQLLVTDLYPVDLFPSLSTMEFKTVEAR"
     gene            complement(6750..8528)
                     /locus_tag="VFF28_02860"
     CDS             complement(6750..8528)
                     /locus_tag="VFF28_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis/translation initiation
                     ATPase RLI"
                     /protein_id="HZX44602.1"
                     /translation="MKVNLRRLLARIAVIEREKIKDKKEWFRLISGVCPINRAGKECV
                     YEKDGQVGINEELCMGCGICVKKDPWQAIQIINLPEELETEPIHRYGANQFCLYSLPV
                     PIFGKVVGIVGVNGIGKSTAIQILAGVLEPNLGQIGKKTDYKKLIDFFRGTEAQVFFE
                     KIRDKQITISYKPQHVDLIPKKFKGKVKDLLTKVDEGKKLGEVVKQLDIENIMDNDIG
                     KISGGELQRVAIAAAVLKKANLYIFDEPSSYLDIKQRLKIAKFIKELANEETSIVVIE
                     HDLIVMDYMADLIHIMYGRQGAYGVVSQLKAAKAGINSYLEGFIQEDNIRFRDHKIMF
                     ETKPPVKKGKEFMLTEWEAMSKKLGKFKLDITPGEVSRHETIGIIGENGIGKTSFVKI
                     LAGVENADKGEMPSAITVSYKPQTLEADSPEIVINVLNDAIKHHTNDIINPLNIPELF
                     TKSLNQLSGGELQRVSIALALSKKCSLCLLDEPSAYLDVEQRLLVSKVIRNFCEHSGI
                     SIMVVDHDLLFIDYLADRLIVFEGEPAIHGVEKGPFSMEEGMNRLLSDVEITLRRDEE
                     SGRPRINKPGSVKDREQKSKGMYYYS"
BASE COUNT         2346 a         2061 c         1530 g         2750 t
ORIGIN      
        1 gaaaattgga ttagcatcga atggtatttc ctcatgtcaa tcagcgacca catggggatt
       61 gctgtttttg tcacgtcagg ccttacgtcc gattgatata tctttttggg gcatcccagc
      121 ttaagatcct caagaaataa aggaagtgtc acctcgcctt cattgagcac aaaatggtcg
      181 atattctcaa actcttcgac cgaagaggta aatagcggtc cgcctgctac gactagcttg
      241 ccttctgttc taaaaatctt tgtaagttcc acaacgcttg atttctgtgc aatcattgcg
      301 ctcacgaaga ccatgtcagc ccaggctata tcgctttttg ccaatttttt tacattgcag
      361 tcgataagcc tctttttcca ctctttagga agcatggaag cgatagtcag cagtcccaaa
      421 ggaggatatg ctgctttctt gagcacatag cttagtacat acttaaaact ccagaatgtc
      481 ttgggatact ctggatagac aagaagtacg ttcataaaat atcaaaaaga taacatcctt
      541 tttatagctt tgtataaact tagccagtaa gtaaagcttt aataataaac acctatcctt
      601 cccacatatg gagattgaaa cccttgcaaa agaagcaaga aacagtatag gggagttttg
      661 catggaagaa tgcaagtcct actgttgcag aaaaggctat ctctcattgt ccaaaaaaga
      721 agcagccttg ttgagcgata atcgcattga tgaatgggtt aaaaaaaggc ttctccacaa
      781 gcttgggaaa aaatactcat ttttcttagg aagcgagcag tttccatgcc ccaagcttaa
      841 agactttaaa tgcactgtcc ataaagatcc aaacaggcca aaagcatgtc aaaattatcc
      901 tattttcatc aaaggaaata agatcaggct ttcctcaaga tgccttgcag taaacgcaga
      961 caagctctat ccttatatca gccagttcct aaaactcggc tataagatag agcatagtta
     1021 ttcattagaa gacgcagata tttatgagaa atagttatac tgttactatt gcctcagtct
     1081 tataatactc atccaagctt tttacagtta ttttctgctt tgcctgcgct ttgattgcag
     1141 caaggcatgg ctttagcgaa gtcatcgtcg taatatatgg gatgtcgagt tcaacgcact
     1201 gcctctttat ggttgcagaa tcgctttttg gatgagtccc tctatggata ttgatgacca
     1261 gctgcagctc tttattcttc atcatctcaa gcacaagggg attttccctt atctttggaa
     1321 ctgtctcgca agctatccca tttgaattaa agcattcagc agtgcttctt gtaccaaata
     1381 gcttaaaccc catggaagag agctgttttg caatcggaac agctgctatc ttatcctcgt
     1441 cctttccgat agttatcaaa atatttcctt cagaaggaag ggttgttcct gaagcagcct
     1501 cagccttaaa aaaagccttt gcaaaattgc tgtccatccc cattacctct cctgttgatt
     1561 tcatctccgg cccaagttca ggtataactc ctgtaagttt caagaacgga aatactactt
     1621 ctttgactga tacaaattta ggctctctat atccgattaa tccaagatcc tttaagcttt
     1681 tgccaagcat tatccttgtt gccatctttg caagaggaag cccgacagct ttgcttacaa
     1741 acggaacagt ccttgacgct cgcggatttg cttcaaggat ataaacaaca tcgtctttca
     1801 cagccatctg tatatttatc agtccgactg tcttaagaga aagtgcaagt ctcgtagtgt
     1861 aatcgatgac tctctccttc aattctttcg acaagttctg aggtggaatg acacatgccg
     1921 agtctcctga atgtattcca gcctcttcta tatgctccat gattgcagcg acaaacacat
     1981 cttttccatc gcaaagcgca tcaacatcca gctcaactgc gttgtcaaga tatttatcga
     2041 taagtatagg atgctcagga gacacattga ctgcttcaat catatagttt tcaagctcct
     2101 catcatcatc aactatctgc atcgcccttc cgcccaggac ataggatggc ctgacaagca
     2161 caggataacc tatccttctt gcgacagcca aagcttcatc atagctaaat gcagtgcccc
     2221 attcagcaga tggtatcttt agctcatccg ttattttccc gaatttattc ctgttttcag
     2281 cgatatctat agcctcaggc tgtgttccaa gtatctttac ccccgcctta tcaagacgaa
     2341 gggccatatt tatcggcgtc tggccgccaa attgaaggat aactcccatt ggattttccc
     2401 gctcgatgat atttagcaca tcctcatatg ttaacggctc aaaataaagc ttgtcagaag
     2461 tgtcaaaatc agtcgagaca gtctctggat tgttgtttat tatcaaagct tcatacccca
     2521 tctcacgtag ggcaaacaca gagtgtacac aggcatagtc aaactcaatt ccctgcccta
     2581 tccttattgg ccctccacct acaattataa cctttttatc attagctgaa ggcattgcct
     2641 catcctcagt ttcataagta gaatatagat aaggggtgta agccttaaat tcagcagcac
     2701 aggtatcgac cattttgtaa gttggaatta tcttgttctc tatcctaaaa tttcttatct
     2761 cttcctcttt cctgccgcat attttcgcta tctggacatc cgaaaaccca ttgcgtttgg
     2821 ctaaaaatat actctctttt tcaagcttga tttctttgcc catctcatag acattcttca
     2881 ctttctcaag gaaccattgg tttataccag tcagcttgct tatctcctca ggactcatcc
     2941 cgatattaag cccgtctctg atatacatca gccttttatg ggttgcaacc ttaaggtttt
     3001 ggattatctc atttttatca gtgcaaattt tatccttagg atcatatcca agtccaaatc
     3061 ttccgacctc aagcgaacgc agggcctttt ggagcgcttc ttcaaaagtc ctgcctattg
     3121 ccattgtctc tcctgttgac ttcatctgtg ttcctatcct tttatcagca gtagcaaact
     3181 tgtcaaacgg ccatcttgga attttgacca caacatagtc tatagtcggc tcaaaacaag
     3241 cctttgtttc ctttgtgaca tcattaggga tctcatcaag cgtcattccg acagctatct
     3301 ttgcagcgat ccttgctatg ggataacctg tcgcttttga agcaagcgca gaactccttg
     3361 acactctggg gttgacttca atgatcctgt actctccagt attataattc agcccaaact
     3421 ggatattgca tcctccttca acctttaaag cacgtattat ttttatggca gatgttcgca
     3481 aagtttgaaa atcatggtca tttaatgtct gtgttggagc agtgactatg ctttccccgg
     3541 tatgtatccc cattggatca aaattttcca ttgggcagac aatgatgcag ttgtcattct
     3601 tatccctcat gacttccatc tcaaattccc cccagccgtt tactccaagg attgcctctt
     3661 caacaagaac ttggtttatg ggtgaaagat tgagcccatg ctgggttatt tccctaagct
     3721 cttcatcagt gtagacgatt cctcctccag ttccgccaag agtgaatgct ggccttatca
     3781 caacaggcag tcctatgtcc ttcacaaaag agacagcctc agaaaccttg gttacagtct
     3841 tgcttcttgg tacaggctcg cctatcttta tcatcagatt cttgaataac tccctatcct
     3901 ccccttcttc gattgaggca attgaagtcc caagcacctc aacattgcat tcagaaagaa
     3961 ttccagtttt agcgacagca accgctagat tcagcccagt ctgtccgccc attgtcggca
     4021 gcagcccgtc gggcttctcc ttgtgtatta tctggcagat tacctcaaca gtaagtggct
     4081 cgatatagat agcatcagcc atgtcaggat cagtcattat tgtagcagga tttgaattga
     4141 taagaacaac tttataccct tcctcacgca gagccttgca tgcttgtgtt cctgaataat
     4201 caaactcagc agcctgccct atcacgatag gaccgcttcc gattacaagt atttttttga
     4261 tatcagttcg ttttggaata agtattcacc cacccaatag ctaatctgtc actcctttct
     4321 ttgtgtaatt taaacagaga ataatcatta actttcttta tctcggcttg ccccataatc
     4381 ctaatctccc ttgttctagg ataaatattt gctattatcc ccaatttgtt aagcacagtc
     4441 atgtagattt gtccctcttc ttgaccatta tttggaatga accctatcat tttagatttt
     4501 tcaccatgat atcaaataag taatcagtat cccatgggcc acagtgagcc tcaggatgaa
     4561 actgccaggc cataatcggc agccgctcat gcctcattcc ttcgacagag ccatcgttaa
     4621 gattgatatg tgaaacggta agcccggtgc catccaggct atcagcatcg actgcaaagc
     4681 catggttctg tgatgtgata aacactttgt tttgtgcaat atcctttaca ggctggtttg
     4741 ttcccctgtg cccaaacttt agcttgtagg ttttcccgcc aagcgcaagg cccacgattt
     4801 gattacccag gcatattcca gtgataggca tctcagactg catttctttg acgacattta
     4861 tcgcatcctt ggccatctca ggatcgccag gcccgtttgc taaaaacagc ccatttacgt
     4921 ttaggcctct gatctcatca gcttttgtgt tgaatggcat cctgtagaca ttcactttcc
     4981 ttttgataaa atttcggatg atgctaatct tgacccctgt atcgatgagt gctatgcttt
     5041 tctctccccc agcatcgata aactctggct tttttacaga aacctttgaa agaaggtcaa
     5101 atgaagaata actctctaac ttctgtgctt ttttgacgac agattcggtg ttgatatcgc
     5161 cttttgaggt gacaagcgca ccatttatta caccgtattc cctgattttc tttgtgagca
     5221 tccttgtgtc tattccagag atcccgggaa tgctatattc cttaagaaat tggctcactc
     5281 ccttgacagc atttctatgt gagaaaaaat cctcatgctc tcttaccaca aatccctcta
     5341 cctggatgaa atcagattca aaattatcat ttctaactcc atagtttccg ataagtggat
     5401 aggtaagtgt cagtatttga cccttatagg acgggtctgt aaggctttcc tcataaccca
     5461 ctaaagaagt attgaagaca acctcccctg ctttctcgct ctcagctcca aagcctatgc
     5521 cttcaaaaac agtcccatcc tttagggcaa ggactgcatt catagcacgg ccagaagatt
     5581 ttgacgtatt tgcccccata taaaaataaa taaggaacaa tgcccattaa tatagttttc
     5641 tattatcaag aagtggtttt aaagacgata aaaaagggtc acccctatca gaacagtaag
     5701 ttacaaatat taaattcttt ataagctttt tgatttccca aactcaaaat tatcttgctt
     5761 caacagtttt aaattccatt gtggataatg atggaaaaag atcgactgga taaagatccg
     5821 ttaccaatag ctgtctacca taagaaagaa ggtgttcaat taaattatca ataacattaa
     5881 aatttcgata agagatatcc tgatcagagc tcagctttgc ccctgctcca gctgcacacc
     5941 ctattattcc cgggctagat gcccaaataa tcggtatagc agtacttgga gggacataac
     6001 ataaagggta atctggtgac actttattca atgctaatcg tgccctaaat aaatcagcaa
     6061 taccttgaaa atcagaagat tttggaaggt ccagtaacat tctaattgat tctgcaaatt
     6121 ccccgaccct tgaaggattt tgcgatatat aactggcaat tgattctcta tatctatcca
     6181 atgtaaaaac ctctgtgata gcatcaggta agattcctat atcttcggga tctaaatgga
     6241 tccatccgct aattatcaga taggataatc tacgataagc actttgcaaa gacaatatat
     6301 catctccagc tttagcactc tccatagctt caattagtgc gaggggtgta taagtggtgg
     6361 ttggactttc tatcacagat agtaaatctt gagcattctt tttttccaaa ggatgtacgc
     6421 taataaatcc aacttcagat ggcataagcc cttctaatgg caatttttca taagcctgta
     6481 ataatagtga ggcatcataa ggtggatcat gtctaaagtg aggcgtaaat ctactcatat
     6541 ctttcatata cacaatagtc aaatctttca tttttcagat agcgtttctt tgatcaatgc
     6601 atcagtactt tctgcttgcc ggtgaattgt catgtaaaga cctcctctaa cttgatggca
     6661 attcttatcc atcatttacc caacccccac aatccttcgg aaaagggaat tggctataaa
     6721 cctttcgtta gaattaccct ctaaaataat caggaataat agtacattcc cttgctcttc
     6781 tgctctctgt cctttacaga cccaggctta ttgattcgag gccttccgct ttcttcatca
     6841 cgtctaagcg taatctcaac atcagataat aaccggttca tcccttcttc catagagaat
     6901 ggccctttct ctactccatg tattgcaggc tctccttcaa aaacgataag cctgtctgca
     6961 agatagtcga taaaaagcaa gtcatgatcg acgaccatta tgcttatccc gctatgctca
     7021 caaaaatttc ttatgacttt tgacacaagc agcctctgct cgacatcaag ataagcagaa
     7081 ggctcgtcaa gcaggcatag gctgcatttc tttgacaaag caagagcgat agataccctt
     7141 tgcagctctc cgccgctaag ttggtttagg gactttgtaa acagctcagg aatattcagt
     7201 ggattgatga tatcgtttgt atgatgcttt attgcatcat taagcacatt gatgactatc
     7261 tcaggagaat ccgcttccaa agtttgcggc ttataggata cagtgatcgc actgggcatc
     7321 tctcctttat cagcgttctc tacccccgca agtatcttca caaaggaggt tttcccgatt
     7381 ccattctcac cgattattcc aatcgtttca tgcctgctga cttcgccagg ggttatatca
     7441 agcttaaact ttccaagctt ttttgacatc gcttcccatt ctgtaagcat aaactctttt
     7501 ccttttttca caggcggctt tgtttcaaac attattttat ggtctctgaa tcttatgtta
     7561 tcctcttgga tgaatccttc aagataagaa ttgatcccgg cctttgcagc ctttaattgg
     7621 ctgacgacgc cgtatgctcc ctgcctgcca tacatgatat ggatgaggtc agccatataa
     7681 tccatgacga tcaggtcgtg ctcgataaca actattgagg tttcctcatt tgcaagctct
     7741 ttgatgaatt tggcgatctt cagcctttgt ttgatatcaa ggtaagacga aggctcatca
     7801 aagatgtaca ggtttgcctt cttaagcaca gcagcagcga ttgcaacccg ctgaagctct
     7861 ccgccagata tcttccctat atcattatcc atgatattct ctatatccag ttgcttgacg
     7921 acctcaccaa gttttttgcc ttcatctact ttggtaagta agtcctttac ctttcctttg
     7981 aactttttgg gtatgaggtc aacatgctgc ggcttatagc tgatagtaat ctgtttgtct
     8041 cttatcttct caaaaaagac ctgggcctct gtccctctaa aaaagtctat cagtttctta
     8101 tagtctgttt ttttaccgat ttggccaaga tttggctcaa gaacacctgc aagtatctgt
     8161 atagccgtgc tttttccgat gccattgact ccgacaattc caacaacctt tccaaagata
     8221 ggcacaggaa gtgagtataa acaaaattgg tttgccccat accgatggat tggttctgtc
     8281 tcaagctcct caggaaggtt gattatctgt atagcctgcc atgggtcttt cttgacgcat
     8341 atcccgcatc ccatgcacaa ttcttcattt atccctacct ggccgtcttt ctcatagacg
     8401 cattctttgc ctgctctatt tatagggcat acccctgaga tgagtctaaa ccattctttc
     8461 ttatctttga ttttctctct ttcgataaca gctatccttg ccaatagcct tctgaggttt
     8521 actttcatta ataaatcttt ttaaactaat tacctgaatt ttccctagtt tttatacaca
     8581 acacctatcg cttccaaaat ctttctctgt tttggctcta attctactgt taggctccac
     8641 tggtgcttgt ttattgagtc atacaactca accttgtatc ctgtttt
//
LOCUS       DASRUK010000060         8399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1395552, whole genome shotgun sequence.
ACCESSION   DASRUK010000060 DASRUK010000000
VERSION     DASRUK010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 8399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8399
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395552"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="VFF28_02865"
     CDS             complement(<1..979)
                     /locus_tag="VFF28_02865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZX44603.1"
                     /translation="MAKSKLEQFIKQPRRSLIYLSIPAIASAMVETFYNLTDTFFIGR
                     LGAEALAAMTFSFPLFFMLVSLSLGINAGMGSRISRYIGEGNKRQAENTAIHGLALSL
                     VISLLIALIAIPLLPFLFKVLGAQGIVLSYAISYTAIILAGSLFMFLSYALSSFFAAQ
                     GDTKTAMKIDVYSLIANMILTPIFMFVFDMGIAGAALATVLSVIYAFVLALYYLRDSY
                     LTLSLKKFKYSSSIIKEIFEVGFPSTLMMLTISFYVVFLNRAMAHFSVEHVAAFGVVS
                     RLETVVTLPVFGLSIGAMTLAGMFFGAKKYKLLKDVSKFSLWLGVGTSII"
     gene            1161..2024
                     /locus_tag="VFF28_02870"
     CDS             1161..2024
                     /locus_tag="VFF28_02870"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HZX44604.1"
                     /translation="MDLKQIALDDKGVFDKFFRGFSPKASEYTFTNIFCWRKPHEHKF
                     AVIGDHLLISSGSSYFQPIGPNPHQAIKSMLNEQSVTFERVEENALGGLPAKEDVAMF
                     DYVYSVEELASLDGAKFASKRNFVNQCKRYKPNTCMLDESTVKSFLSLAEEWCDMRGC
                     KDDPNVAAEDFALREALANYKVLGLFGICINVEGKIAAFALGERLNDDTFVEHFEKAD
                     TRIKGIYAYLLNELAKALAGRYRYINREQDLGVEGLRKAKQSWNPVCMIKKYRITNVK
                     NRRAEKPGACS"
     gene            complement(2073..2303)
                     /locus_tag="VFF28_02875"
     CDS             complement(2073..2303)
                     /locus_tag="VFF28_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44605.1"
                     /translation="MSEIASGARSDVSEIAYGTYFAHALSMTHQAISEHAQKLITNRQ
                     KWGVFDTLAQSKPAISECIEMQSISNAQEIWE"
     gene            2346..2645
                     /locus_tag="VFF28_02880"
     CDS             2346..2645
                     /locus_tag="VFF28_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44606.1"
                     /translation="MDRTLTSGEGATTVECIVNLPSGMPAGKAREFIAQMDVGHWCGN
                     GFPKCRDFKVLGGTGVISEETLQIRCNRDDVGHLQRALPEYALERYKRNCVYNTQ"
     gene            2728..4113
                     /locus_tag="VFF28_02885"
     CDS             2728..4113
                     /locus_tag="VFF28_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HZX44607.1"
                     /translation="MKAVLSNMGFVMQMSGIFILIPIILSFVYKENAATIALFITATL
                     FFALGFLLNALCERKELNFKESSSLIVLVFILLSIIGAIPYLYLGINDGSLMQRVTNS
                     IFESASGFTTTGFSVIPDLSAIPKSIIFYRSLTQFLGGLGIVLVLLAFFYPSDKLEEF
                     SRSMGFMKNGKIKKMLVIVLMIYFIYSLLLTGICFALGYKDIISLVSLIFSALSSGGF
                     TPTNQLSVPYPINYGLVIGMILGASNFIVIAKLAKFRFRQFFQSEIPVLLTIIFGAFL
                     AVKCLFNLSYFDSLFHIVSASTTTGFQYLPLAEMSDSLKLFLTFLMFIGGTSLSTAGG
                     IKIYRFILIFKSFKKAIHDSITREDRPIKQFGCEYTNSDVINALAIIILTIALAFVSA
                     LILTYYGFGLVDSLFEATSAIATTGLTVGIVTPAIALELKWLFITLMILGRVEIIAFF
                     IMLSRARQSKT"
     gene            complement(4110..4514)
                     /locus_tag="VFF28_02890"
     CDS             complement(4110..4514)
                     /locus_tag="VFF28_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44608.1"
                     /translation="MPEPKRSGEQNNTLEGLAGTIDDWASADAARDVKQKIEQGIYDI
                     LYGFFRDRKIRDTAARYLVERGIGVLPEQQVYSYLTSLTPDGIQPHGAKCNPANNTAY
                     FMRAHQLERQKFDSMYLQTIEDITVPKTRQKD"
     gene            complement(4597..5802)
                     /locus_tag="VFF28_02895"
     CDS             complement(4597..5802)
                     /locus_tag="VFF28_02895"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HZX44609.1"
                     /translation="MTTLTTLLKELFPFQGYKLSLLGTKNRIVIGMESLHKGGICPKC
                     GKRCLCIEEEYERLVRDLDMCPTKCFLHFWQKKIRCRCGYRGIERLDFLAKYDRVTTR
                     LADAVASDCEEATLKEVARRHDLNWKSVKEIDREHIKLLLPKIIDLKIRRIALDEIAI
                     MKGHKYFTVIRDYDTGVAIKIIFGRGYQETYDALLSLGKDTLSQIKFASLDMWDPYIK
                     AISELCPDAKLVFDKFHVVKKVNAAVDKVRKTEFAKASDEERINMKHKRWIILKREIN
                     LNTEGREELDALLKNNDKLYKAYLLKEQILSIFDDKVSDFETIQLRIKCWFENIFSNG
                     LEEFNEVMNTMQHYLEGILNYFRYGMTNAIAEGFNTKINVIKRRAFGFKDLEYFMLKI
                     YQSSLRRLA"
     gene            complement(5877..6752)
                     /locus_tag="VFF28_02900"
     CDS             complement(5877..6752)
                     /locus_tag="VFF28_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44610.1"
                     /translation="MKKLLLISLLLLIMPVAFAEDVDVNVYLLNLGKYEISTGSFTAD
                     FYLSMKCESDCGRFEFMNGRADKIDAITDEPNEKFYRIQAILSSPVDLAGFPFDKQKM
                     QIIIEDKEKTIEDIKYIATKEKSGLDESIAFSGWQIDGWDIATKEHYYPPYDETYSQY
                     VYTLNISRIPLNAFIKTFLPVFFMILIVMFTFIMDTDKIATRIAVTSSSLVAAVMFHI
                     SVANQIPPVGYLTVADKFMILTYFIILLSVVSNVMILEFMEQKKIELAEKIHRRTEYS
                     VFIIIPLMYLLFFIF"
     gene            complement(6793..7278)
                     /locus_tag="VFF28_02905"
     CDS             complement(6793..7278)
                     /locus_tag="VFF28_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HZX44611.1"
                     /translation="MSDTEEVFIEIPKGCRNKYEFDAKAGVIRFDRMLYSSVQFPSDY
                     GFIKGTLAKDGDALDALVLVKEPTFPGCHIEAIPIGVFIMSDEHGMDEKILCVPVNDP
                     TSNHMKRLKDVPPHLLKEIQQFFSTYKALEGKKVKVEGWQDRPEALKIISEAKKRYAT
                     R"
     gene            complement(7333..>8399)
                     /locus_tag="VFF28_02910"
     CDS             complement(7333..>8399)
                     /locus_tag="VFF28_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44612.1"
                     /translation="HGPTARLWALWRQQLAYPKFAEGVRSICSTYPIPVNAHGLPFII
                     SPLTHRGDSTGNGRALHEIIEPDIVHLDRLPHPKYKGIIADGEFLTVSNTPASIPHCI
                     AWAMPSSEYDVRLLEGHYPRQSVWDSADEMLRLADMHPGVTIISHGVLSGSSKSNRHQ
                     QMYIDTFIPCQRLLYKLAFGEKRCGLDSKILVKRKHSLLLDVIGYLPCQMLIAKDQSA
                     VKGFLEKYHPTLANLDKDFLSKSTAPDHVKPYGLWVPGLDCFEYDPRVGVTVRFLDGT
                     YIVECFPKLTNRPSPNTTLIGIGPATRESQGQVIALNRNDAMLLATQPELLWRILYET
                     STAYIPAMNKELAYQLRKGF"
BASE COUNT         2402 a         1751 c         1697 g         2549 t
ORIGIN      
        1 tgatgataga tgtgccaact cccagccata gggagaactt gcttacatcc ttaagaagct
       61 tatacttctt tgctccaaaa aacatcccgg ctagtgtcat agctccgata gagagcccaa
      121 acacaggaag cgttacaaca gtctcaagcc tgctcacaac gccaaaagct gcaacatgct
      181 ccacgctgaa atgagccatc gcacggttta agaaaacgac atagaaggaa atagtcagca
      241 tcatcaaagt tgatggaaaa cccacttcaa agatttcttt aatgatgcta gacgaatact
      301 tgaacttttt aaggctaagc gtgaggtaag aatcccgaag ataatacaag gcaagaacaa
      361 atgcatagat gacagacaat actgttgcaa gggcagctcc tgctatgccc atatcaaata
      421 caaacataaa aattggtgtt aggatcatat tagctataag cgaatagacg tctatcttca
      481 tcgctgtctt tgtatctccc tgtgcagcaa aaaagctgct taaagcatag gataaaaaca
      541 tgaataaaga ccctgcaagt attatggctg tataagagat agcatagctt agcacaatac
      601 cttgtgcgcc aaggactttg aataaaaatg gcagtaaggg aatggcgatc aaggctatca
      661 gcaaagatat caccaatgat aatgcaagcc catgtatcgc tgtgttctca gcctgcctct
      721 tatttccctc gccgatgtac ctggatatcc ttgaacccat tccggcattt atcccaaggc
      781 taagtgagac aagcataaag aacaatggaa atgaaaaagt catagcagca agcgcttctg
      841 cgccaagcct tccgataaaa aaagtatctg taaggttata gaacgtctcg accattgctg
      901 aagctatcgc tgggatgctc agatagatta atgacctcct tggctgcttg ataaactgct
      961 ctagcttgga tttagccatt ttatggttgt tacgggcttg tcaagaactc cgttttgaaa
     1021 agcagggcgt gctatcgaga gttcttgagc atgctcagaa atcttcttca agttatgaag
     1081 ctcttgcttc ttgtagccct cctcaggttg gatagaagat ttctgacata tttaaatatt
     1141 gggattgact tagtgggttt atggacttaa agcagatagc gcttgatgat aaaggggttt
     1201 ttgacaaatt ctttagaggg ttctctccaa aggcgtcaga gtatacattc accaatatat
     1261 tttgctggag aaaaccacac gaacataagt ttgcagttat tggcgatcac ttgctcatct
     1321 catcaggaag ctcctatttt cagcccatag gcccaaatcc tcaccaggca atcaagagca
     1381 tgctaaatga gcaaagcgtc acctttgaaa gggtggagga aaatgcattg ggtggattac
     1441 ccgcaaaaga agatgtggca atgtttgact acgtatattc tgtagaagag ctggcatcac
     1501 ttgatggcgc caagtttgcg agtaaaagaa actttgtcaa tcaatgcaaa agatacaagc
     1561 caaacacttg catgcttgat gaaagcacag tgaagagttt cctttctctt gcagaggaat
     1621 ggtgtgatat gaggggatgc aaagatgacc ccaatgttgc agctgaagac tttgcgctaa
     1681 gggaggcatt ggctaactat aaagtgctgg gattgtttgg catctgcata aacgttgagg
     1741 gcaaaatagc agcttttgct ttgggggaaa ggcttaatga cgacactttt gtggaacatt
     1801 ttgagaaggc agacacaagg ataaaaggga tttatgccta tcttcttaat gagcttgcaa
     1861 aagcactggc aggcagatac aggtatataa acagagagca ggatttaggt gttgaaggac
     1921 taaggaaagc aaagcagagc tggaatccgg tctgcatgat caaaaagtat aggataacaa
     1981 atgtcaagaa ccgccgggct gaaaagcccg gggcgtgttc ttgagcatga actgagaaat
     2041 ttggcaagca aatttctggt tccctaagaa atttattccc aaatttcttg ggcgttagaa
     2101 atgctttgca tttctataca ctcagaaatc gctggcttac tctgggctaa agtgtcaaaa
     2161 actccccatt tttgcctgtt tgtaatgagt ttttgagcat gctcagaaat cgcttgatgt
     2221 gtcatagaca aagcatgagc aaagtatgtg ccataggcga tttctgacac atcagagcga
     2281 gcgcccgaag cgatttctga catgtcaact taaatttata aatgacaagg taactgtaaa
     2341 aaagtatgga tagaacacta actagcggag aaggagctac cactgtagaa tgcattgtta
     2401 atctgccaag tggtatgcct gcgggcaagg cacgtgaatt tatagcgcag atggatgttg
     2461 gccattggtg tggcaatgga tttcctaaat gcagagattt caaggtatta ggtggaacag
     2521 gtgtaatttc agaggaaaca ttgcagatta gatgtaatag ggatgatgta ggacatcttc
     2581 aacgtgcatt gccagaatat gcgctggaaa ggtataagcg caattgtgtg tataatacgc
     2641 aataaaccgt tttttcaatt gtgcaattgc ctgtctgttt aagctaaatc caaaagatta
     2701 atatatagtc ttctttcgtt tcaatgtatg aaggcagttc tttcaaatat gggctttgtc
     2761 atgcagatgt cagggatatt tatcctgatt cctatcatcc tttcctttgt ctataaggaa
     2821 aatgcagcca ccatagctct ttttataaca gccacgttgt tctttgctct tggtttttta
     2881 ttaaacgcat tatgtgaaag aaaagagctg aatttcaagg aatcaagcag ccttattgtc
     2941 ctggtattca tcctgctcag tataatagga gctattcctt atctatatct gggcatcaat
     3001 gatggtagtt tgatgcagag agtgacaaac agcatctttg aatcagcctc agggtttaca
     3061 accactggat tttcagtcat ccccgacttg agtgccatcc ccaagtcaat tatcttttac
     3121 cgttccctga ctcaattcct gggaggatta ggcatcgttc ttgtgctcct ggcattcttt
     3181 tatccaagcg ataagcttga ggagttctca aggagcatgg gatttatgaa gaacggcaag
     3241 ataaagaaga tgcttgttat agtcctcatg atctatttta tatacagtct cttgctgaca
     3301 ggaatttgct ttgccttagg ctataaggac attatcagcc ttgtctcact tatcttttct
     3361 gcacttagct caggaggatt tactcctacc aatcaactct cagtccctta cccgatcaac
     3421 tatggccttg taataggcat gatccttgga gcatctaatt ttattgtcat agcaaagctt
     3481 gccaagttca gattcaggca gttctttcaa tctgagatcc ctgttttgct caccattata
     3541 ttcggtgctt ttttggctgt gaaatgcttg tttaacctaa gctacttcga ttcgcttttc
     3601 catattgtta gcgcctcgac cacgactgga tttcaatacc tgccccttgc agaaatgagc
     3661 gattcattaa aactattcct tacctttctg atgtttatag gcggaacaag cctctcgaca
     3721 gcaggcggca taaagatata tagattcata cttatcttca aaagcttcaa aaaggccata
     3781 cacgactcta taacccggga agacaggcct atcaagcaat ttggatgtga gtacacaaat
     3841 tcagatgtta tcaatgcgct tgccataata atactgacga tagccctagc ctttgtctca
     3901 gcgctgatac ttacttatta cggctttggg cttgttgatt ccctgtttga ggcaacatca
     3961 gctatagcta cgaccggttt gactgtagga attgtcacgc cagcgatagc tcttgagcta
     4021 aaatggctat tcatcaccct catgatcctt ggcagggttg agataatagc gttttttatt
     4081 atgttatcca gggcaagaca atcaaagact taatcttttt ggcgggtttt agggacagta
     4141 atgtcctcaa tcgtttgtag atacatagaa tcaaactttt gtctttctaa ttggtgtgct
     4201 ctcataaagt atgcagtatt attggcagga ttgcattttg cgccatgagg ttggataccg
     4261 tcaggagtca aagaggtcag atatgagtat acttgttgct caggaagtac tcctatgcct
     4321 ctctcaacta aatatcttgc ggcagtatcc cgaatcttcc tatctctaaa aaatccatat
     4381 aggatatcat aaatcccttg ctcgattttt tgtttaacat ctcttgcggc atctgcactt
     4441 gcccagtcat ctatcgtacc tgcaaggcct tcgagagtgt tgttttgctc acctgaacgc
     4501 ttgggctctg gcatatgcct cccttgcgga aaaagttgga taattcttgc catctcaact
     4561 taagaataag cttattatta tggctcttct cccattttag gcaagcctcc gcaatgagct
     4621 ttgatatatt ttcagcataa aatactccaa atctttaaac ccgaatgctc ttcttttgat
     4681 aacatttatt tttgtgttga acccttcagc aattgcatta gtcataccat acctgaaata
     4741 gtttaggata ccctccaaat aatgctgcat tgtattcatc acttcattaa attcttccaa
     4801 gccattactg aagatattct caaaccaaca tttaatcctt aactgaattg tttcaaaatc
     4861 acttaccttg tcgtcaaaga tggaaagaat ctgctctttg agcagataag ctttgtatag
     4921 cttgtcatta ttttttaata gagcatctag ctcttcccta ccctcagtat taagattgat
     4981 ttctctcttc agtattatcc acctcttatg cttcatgtta atcctctctt catcacttgc
     5041 cttagcaaat tcagttttcc tcactttgtc aactgcagca ttaaccttct tgacaacatg
     5101 gaatttgtca aaaacaagct ttgcatcagg gcaaagctca gatatcgctt taatatatgg
     5161 atcccacatg tcaaggcttg caaacttaat ctggctaagt gtgtcttttc ctaggctgag
     5221 caacgcatca tatgtctcct gataacctct gccaaagatg attttgatag ctacaccagt
     5281 atcatagtct cttatgactg taaaatattt gtggcctttc attattgcta tttcatctag
     5341 ggctatgcgc ctgattttca ggtcaattat cttaggtagc agaagcttga tatgctccct
     5401 atctatctct ttgacactct tccaattaag gtcgtgtctt cttgctactt ccttaagcgt
     5461 ggcttcttca caatcactgg ctacagcgtc ggctaacctt gtggtaaccc tatcatactt
     5521 agctagaaaa tctagccttt ctatacccct atagccacat ctacatctta ttttcttctg
     5581 ccaaaagtgg agaaagcatt ttgttgggca catgtctaaa tctcttacca accgttcata
     5641 ctcttcttct atacaaaggc atctttttcc gcatttaggg caaattccac ccttgtggag
     5701 gctctccatc cctataacaa tgcggttttt agttcccaaa agggaaagtt tatatccttg
     5761 gaaaggaaat agttccttta acaaggttgt caaggttgtc atcctcggga aaccgagggt
     5821 ttcaacctat aaattttatc ctaaattagg agaagaaaca ttattataaa atgttactaa
     5881 aaaataaaaa acagcaaata cattaaaggt attattataa aaacgctata ttcagtcctt
     5941 ctatgtatct tttcagcaag ctctatcttt ttctgctcca taaactctag tatcattaca
     6001 ttgctgacta cagacaataa tattatgaag taagttaata tcatgaactt gtctgctact
     6061 gtaagatagc caacaggagg tatctggttt gcgaccgaga tatgaaacat cacagctgca
     6121 acaaggcttg agcttgtgac tgctatcctt gtagctatct tgtctgtgtc cattatgaaa
     6181 gtaaacatga cgataagtat catgaagaac acaggaagga atgtcttgat aaaagcgttg
     6241 agaggaatcc ttgagatatt tagggtatag acatattggc tgtaggtttc atcatacgga
     6301 ggatagtaat gctcttttgt tgcgatgtcc catccatcta tctgccagcc gctaaaggcg
     6361 atagactcgt caaggccgct tttttctttg gtcgcaatat acttaatatc ctcgatcgtt
     6421 ttctccttat cttcgatgat tatctgcatc ttctgcttgt caaaaggaaa tccagcaagg
     6481 tctacagggc tggatagtat tgcctgtatc ctatagaatt tctcgtttgg ctcatctgtg
     6541 atagcatcta tcttatctgc cctgccgttc ataaactcaa acctgccgca gtcgctctcg
     6601 catttcatag aaagataaaa gtctgccgta aatgagccgg tgcttatctc atattttccc
     6661 agatttagca gatatacatt tacatcaacg tcttctgcaa aagctaccgg catgatcagt
     6721 agcaataaag atattaacaa tagtttcttc ataaccttca gaatcataga agtggtttat
     6781 ttagttttcg ctttatctag tcgcataacg cttttttgcc tcagagatta tctttaacgc
     6841 ctcaggcctg tcctgccagc cttcgacctt gacctttttg ccttcaagtg ctttgtatgt
     6901 agaaaagaac tgctgtattt ccttaaggag atgaggaggg acatctttta gtctcttcat
     6961 gtggttggat gttggatcgt ttacaggaac gcaaagtatc ttttcatcca tgccgtgctc
     7021 gtctgacatt ataaagactc ctataggtat tgcctctata tggcagcctg gaaacgtagg
     7081 ctcttttaca agaacaagag catctagtgc atctccgtct tttgccaggg tgcccttgat
     7141 aaaaccataa tcgcttggaa actgcaccga agaataaagc atcctgtcaa acctgatgac
     7201 gcctgctttt gcatcaaact catacttgtt cctgcatccc tttgggattt cgataaatac
     7261 ttcttctgtg tctgacatga aaggtaacaa atcactaagt gatttatata gttaactaaa
     7321 aaatggtgtc acctaaaatc cttttctcaa ttgataagcc agctctttat tcatagcagg
     7381 aatatatgct gttgatgttt catatagtat tctccaaaga agctcaggct gcgtagctaa
     7441 aagcatagca tcgttacgat taagggctat gacttgcccc tgtgactctc ttgttgcagg
     7501 cccgatacca ataagagtag tattagggct tggcctgtta gtcaactttg gaaaacactc
     7561 gacaatataa gtgccatcaa gaaagcggac tgtaacaccg actcggggat cgtattcaaa
     7621 gcaatcaagt cctggaaccc acaggccata aggcttgacg tgatctggtg ctgttgattt
     7681 tgaaaggaag tccttatcca agttcgcgag cgttggatga tatttttcaa gaaatccctt
     7741 taccgctgac tgatcctttg ctataagcat ctggcagggt aaatagccga tgacatctaa
     7801 taagagcgaa tgttttcttt tcaccaaaat cttagaatcc agcccgcatc ttttttctcc
     7861 aaaagcaagc ttgtaaagaa gtctttggca ggggataaaa gtgtcgatat acatctgttg
     7921 atgcctgttg gacttagagg agcctgaaag tacgccgtgg gatatgatag tgacgcctgg
     7981 atgcatgtct gcaagcctaa gcatctcatc agcgctgtcc caaactgact gccttggata
     8041 atggccttca agaagcctca catcatattc agaagaaggc attgcccatg ctatgcaatg
     8101 aggtatgctt gcaggagtgt tgctgacagt tagaaattca ccatctgcaa ttatcccctt
     8161 atatttagga tgaggcagcc tgtcaagatg gacaatatca ggttcgatta tttcatggag
     8221 cgctcttcca ttgcctgtgc tgtctcccct atgagtcaat ggtgagatta tgaaaggaag
     8281 cccatgagca ttgacaggga ttggataagt ggaacatata gatctgacgc cttctgcaaa
     8341 tttcggatag gctagttgtt gcctccaaag agcccataac ctggcagtgg gtccatgcc
//
LOCUS       DASRUK010000061         6238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1721653, whole genome shotgun sequence.
ACCESSION   DASRUK010000061 DASRUK010000000
VERSION     DASRUK010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 6238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6238
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1721653"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(322..1038)
                     /locus_tag="VFF28_02915"
     CDS             complement(322..1038)
                     /locus_tag="VFF28_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44613.1"
                     /translation="MAEPAQTKSLEQRVGIFESVGKTLEGLVTDVKQLAVDSVFYGYY
                     GAKAAAGIAAGLAIAGTPRILTFPLGMALGSYLNKKISKTPMSFKDSYKHVMNELAVG
                     GVLAGFLNYLFKGVGFVADIFKANYGKMAGLAAGALGSLATTPGFMATHEYLNRFLVS
                     GYESKPLKDIPKNMFGPLLPTLPLVMANYSVVPEYLGPTYQMPVASGISTLYGMLKGG
                     NKKKEEKTPLPQPGMQYAGG"
     gene            complement(1031..1183)
                     /locus_tag="VFF28_02920"
     CDS             complement(1031..1183)
                     /locus_tag="VFF28_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44614.1"
                     /translation="MKHRISISIDETTIFAIQDALRQNSRSGVFRNKSHLIEQAVKKY
                     LEEKDG"
     gene            1454..1975
                     /locus_tag="VFF28_02925"
     CDS             1454..1975
                     /locus_tag="VFF28_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44615.1"
                     /translation="MLSVSKNEFNIIGFLARNFSKKFTIRNIASELKVSAPGAHASLK
                     KLEKNNIVKAEKLGTGLFYEIDYANKTAYHLAAASLLDHFELKNISIKELEKEAKAAL
                     FDGKEAIIITNSPDNANAICSKMEIKCSCLSEDEFLEMLKVKDENVIHIIQKGNVLFG
                     EEQVINLIKKVIR"
     gene            1972..6123
                     /locus_tag="VFF28_02930"
     CDS             1972..6123
                     /locus_tag="VFF28_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44616.1"
                     /translation="MKRTRIILLVLAALALLSPVSAGLFDIGMNSFENFFSGGWLNYD
                     KTITFVVFFFLFFTAFLIGLKNAMKEITRPHTAFAFFAALLASFIITTTYAIDLTILK
                     WIAWGLLALLLFYILYALLSKTVLKDKPFWAFLLALLLTALILGLIWYLAQQGRPLEG
                     FKIANPFANWKMPSISQGKTPKKTPLDRPPITGKETKPGTTPGETPGKSMLAKYWWIL
                     PLIPLIVGGGIYGVSRARGRRRGQADRGGGETPGREDDPRINFLNHVLSRKQMVLKNM
                     AGIEEQKQKIDDKLPTIKAELAKIKGKDITYFMDNIAPYLRSPKSEEYKKRRLEARGI
                     DKLIIDNHIMMRLFRVLFKTEQSVISAVSQFRSDNAEMMQRWARLEEISSQIIILLRD
                     LFNIGSRQRITEENLKELLSDRNIENRVSEKWIKTAEAQEDTHSQLLKQELDNFAKVK
                     PLLEQEIAILTQLIGEHRRQPALISISITNPKPGQRFDPGAEIPLKAEVSGININNPQ
                     YTLRWFMRDSENYEENTDPKKETLKMSNNGRAQKIAELQIALRAAQGTTNTKLFRQTQ
                     ERELALLQKGNNDPIAMGTKVTAMVPADFKIGKAYIYAKAYRIYVENGMYKYKEIAMS
                     GMLEVNIGEVEPEIKPKVTITSPHEGREHNTFTLGQEVEFDAECPGAKDLPGGAMVPG
                     WRWKVDGEVKWDRKKFVLKTADYSEKVYKITVEAYCGEQVGWVSSTERKIKIKGKTAE
                     APVIEGIIVEPAQALQKNGGVPVGQPVKVAAKITGGDLPSEYESWIEVAGENKGKLAE
                     GKLHSGTTYPTEVLIDNKPFTTEGVYNIKFKIRNNKKNVVEAEATILIKPAEKESAEL
                     EELRKQIQKLIESMDKDVSEELGELIRKILHSELDRLAQVVKNYKKDEIKEMIEKILE
                     IIEAIKQADNKDGKKRGLIALYDSIVALSKQVDSIKDIIKVIAEINKGIKKILSEDEE
                     RDDRGGGRGRGGSGGGVNSRIRVGDIIINGGGGGSGTVDLAGYSFTITKEGKSPRKVQ
                     EGKEYADFVFKIENKGRLDDSYKLGAIIYKGQYAKDNIDDSWGKVDIYLIDPKHRKER
                     MDDRVDLHNNDERDIRLSLKVPDSAEGKYYLRLVGRSDGAWRHKVRKTAEDKIILEIG
                     KDDKDDEDRGKKEYENKKSPLSKLVFPNNPAGLIGFAFSHHEDTKYITLDRLEAHYQK
                     AKKNKESVESSHQQLANEIRLDAISFPKGRYDIPVKGEFEGKAFARDRFRDNPYVNNK
                     MNIPEVFSQIDALYRDINSKWSDFDKVVRESAKIAEKKGASIGVFDNGQWDGINPKNA
                     IELVMWVNNRLDPKKRYKLEDDQKGVLRQFAYLTEILSLDWDTFELGVWEIDRLIEKI
                     YKKKK"
BASE COUNT         2085 a         1090 c         1392 g         1671 t
ORIGIN      
        1 tgtgtgaatt tctatgtgtg ccataaaatt atcacctttg aaataattaa cggctacata
       61 tatttaaact tatcggtgat agaatctata tgtagcaggc aatatattaa gaagtattat
      121 taagggtttg gtctacttaa ctgatattgt ggcaaatctt ggccattttc tttttgtatt
      181 ttgcattctt cgcccatcca tataactcca gtttgacagt taaatggaaa aagtattctt
      241 agttgtttga ctctaccctg aaattgccac ccgtatggct atactgcaag cttattcttg
      301 aacctatcgg ggatgagtag tttagcctcc cgcatattgc attcctggtt ggggcaaagg
      361 cgttttttct tcttttttct tgtttccacc tttcagcata ccgtaaaggg ttgaaattcc
      421 tgaagcgact ggcatctggt atgtaggtcc aagatactca ggaacaactg aatagttggc
      481 catgaccaac ggcaaagtcg gcaagagggg tccaaacata ttttttggga tatccttaag
      541 aggttttgac tcatatcctg aaacaagaaa cctattaaga tattcgtgag ttgccataaa
      601 acctggagtt gtagcaagag agcctaaagc tccagccgcc agacctgcca tttttccata
      661 gtttgccttg aatatatctg cgacaaaacc gactcccttg aaaagatagt taaggaatcc
      721 tgctaaaacg cctcctacag caagctcgtt catcacatgt ttatagctgt ctttgaagct
      781 catcggtgtc ttgctaatct ttttgttaag atatgagcct agtgccatac caagaggaaa
      841 tgttaaaatc cggggtgttc ctgctatcgc aagacctgct gcaataccag ctgctgcttt
      901 agccccatag tatccataaa atacactatc tactgcaagc tgtttgacgt cagtgacaag
      961 gccctctaga gtcttaccaa cagattcgaa gatgcccacc ctttgctcca aagatttagt
     1021 ttgcgcaggc tcagccatct ttctcctcca gatatttttt gactgcctgc tctatgagat
     1081 gggatttatt cctaaaaaca ccagacctag aattttgcct tagagcatcc tgaatagcaa
     1141 agatagtcgt ttcgtctatc gagatgctta tcctatgttt catttgttac cactaattag
     1201 tgtgaaaagt tattactgat atataaagct tattaattta gttactaaat agtgattaat
     1261 tatattttgt tctaattaat ctaacactag ttaataataa actaattatc ataagtattt
     1321 tatattcatt aataattgga ttaattacta tgtatcatcc aaatattact atagcttatt
     1381 ttaaacagat gctaaataga aatatttaaa taaaggtata ttaattacta attaatatta
     1441 gatataactg aacatgctat cagtaagcaa gaatgaattc aatataatag gatttttagc
     1501 taggaatttc tctaaaaaat ttaccataag aaatatcgcc tctgagctta aagtgagcgc
     1561 gcctggtgca catgcaagct taaaaaaact tgagaaaaat aatatcgtaa aagccgagaa
     1621 gcttgggaca ggcttattct atgagatcga ttatgctaat aaaacagcat accatctggc
     1681 tgctgccagc ctgcttgacc attttgaact gaaaaatatc agtataaaag agcttgagaa
     1741 ggaagcaaag gctgcattat ttgacggtaa agaggctatt attatcacta attctccaga
     1801 taatgcaaac gctatctgca gcaagatgga gataaaatgc tcttgcttaa gcgaagatga
     1861 gtttttggag atgctcaagg taaaagatga aaatgtgatt catatcatac agaaaggcaa
     1921 tgtgcttttt ggagaagagc aagtaatcaa tcttatcaag aaggtgataa ggtgaaaagg
     1981 acaaggatca ttctgcttgt tttggcagcc cttgcactgc tatcccctgt aagcgctgga
     2041 ttatttgata tagggatgaa cagctttgaa aatttctttt caggcggatg gcttaattat
     2101 gacaagacca taacatttgt cgtcttcttc tttttgttct ttacagcttt tttgataggc
     2161 ttgaaaaatg ccatgaaaga gataacaagg ccacatacgg cttttgcgtt ttttgctgcg
     2221 ctccttgcat cattcataat caccaccact tatgcaatag accttactat ccttaagtgg
     2281 atcgcatggg ggcttcttgc gctcctgctg ttttacatat tatatgccct gttatcaaag
     2341 acagtgctta aggataaacc attctgggca tttcttcttg ctttactgct tactgccctg
     2401 atacttgggc ttatctggta tcttgcgcaa cagggaaggc cgcttgaggg atttaagatt
     2461 gctaatcctt ttgccaattg gaaaatgcca tctataagcc agggcaaaac ccctaaaaag
     2521 acgcctcttg atagaccgcc tatcacagga aaagagacaa agcctggaac gacacctgga
     2581 gagacgcctg ggaaatcaat gcttgcaaaa tactggtgga tactgcctct gattccgctc
     2641 attgtcggcg gagggatcta tggtgtaagc agggcaagag gaaggagaag aggacaagct
     2701 gatagaggag gcggagagac acccggcaga gaggacgatc ccaggataaa tttccttaac
     2761 cacgttctta gccgcaaaca gatggtccta aagaatatgg ccggaattga agaacaaaaa
     2821 cagaagatag atgacaaatt gcctaccata aaagcggaac ttgccaagat aaaaggaaag
     2881 gatatcactt actttatgga caatatcgcc ccttatcttc gctctccaaa aagcgaggaa
     2941 tataagaaaa ggagactgga ggcaagaggt atagataaac taatcattga taaccacata
     3001 atgatgcggc tttttagagt gctgttcaag acagagcaga gcgtgattag tgctgtcagc
     3061 caatttcgtt ctgacaatgc tgagatgatg cagcgatggg caaggctaga ggaaatatcg
     3121 tctcaaatta tcattcttct tcgagaccta tttaatatag gctccaggca aaggataact
     3181 gaagagaacc taaaagaatt gctaagcgac agaaatatcg agaaccgtgt ttctgaaaaa
     3241 tggataaaga ctgctgaggc tcaggaagat acgcactctc agcttcttaa gcaagagctt
     3301 gataattttg ccaaggtaaa acctctgctt gagcaggaga tcgcaatatt aacccagctt
     3361 atcggagagc acaggagaca gcctgccttg atatctatct cgataacaaa ccctaagcct
     3421 ggacaaagat ttgatcctgg agctgagata cctctcaagg cagaagttag tgggatcaac
     3481 attaataatc cacaatatac tttaagatgg tttatgagag attctgaaaa ttatgaagaa
     3541 aacacagacc caaaaaaaga aaccctaaag atgtctaaca acggaagggc tcagaaaatc
     3601 gctgagcttc agattgccct cagggcagcg caaggaacga caaatactaa attatttcgg
     3661 caaacgcaag aaagagagct agcattacta cagaaaggaa ataatgatcc aatcgcaatg
     3721 ggtactaagg taaccgcaat ggtcccagct gactttaaga taggaaaggc gtatatctat
     3781 gccaaggcat ataggatcta tgtagagaat ggtatgtata aatataagga gattgcaatg
     3841 tctggtatgc ttgaggtaaa catcggtgaa gtcgaacctg aaatcaagcc aaaggtaacg
     3901 atcacttcgc ctcatgaagg aagggaacat aacaccttta ccctcggcca agaggttgag
     3961 tttgatgctg aatgccctgg tgccaaggat ctgcctggcg gcgcaatggt gccaggttgg
     4021 cggtggaaag ttgacggcga ggtaaaatgg gataggaaga aatttgtctt aaaaacagct
     4081 gactatagcg aaaaagtgta taagataaca gttgaagctt actgcggtga acaggttgga
     4141 tgggtatctt ctactgagcg caagataaag atcaagggga aaacagcaga agcgcctgtg
     4201 atagagggga ttattgttga gcctgcccaa gctctgcaaa agaatggtgg agtgcctgtg
     4261 ggccagcctg tgaaagtagc agcaaagatt actggcgggg atttgccctc ggagtatgaa
     4321 tcatggattg aagtcgctgg cgaaaacaag ggtaaactgg cagaaggcaa actgcattct
     4381 ggtacaacat atcctactga agtgctcata gacaataaac cgtttacaac tgaaggagtt
     4441 tacaatatca agtttaagat aagaaataat aaaaagaatg ttgttgaggc agaagctact
     4501 attcttatca aacctgctga gaaagaaagt gcagagttgg aggaattaag aaagcaaatc
     4561 cagaaattga tagaaagcat ggataaggat gtctctgagg aattaggcga gctgatcaga
     4621 aagatactcc attctgagtt ggatagatta gcccaagttg taaaaaatta caagaaggat
     4681 gaaataaaag agatgattga gaagatattg gagataattg aagcaattaa gcaagctgat
     4741 aataaagatg gaaaaaagcg aggacttata gcgctttatg atagtatagt tgctctttca
     4801 aaacaggttg attccatcaa ggacattatt aaagtcatcg ctgagatcaa taaaggcatc
     4861 aaaaagatac tctctgaaga tgaagagaga gatgatagag gtgggggcag aggcagagga
     4921 ggctctggcg gcggagtaaa ttccaggata agagtgggag atataataat aaatggcggt
     4981 ggaggtggca gcggaactgt tgacctagca gggtatagct ttaccataac caaagaaggt
     5041 aaaagccctc gaaaagtgca agaaggaaaa gaatatgctg attttgtgtt taaaattgag
     5101 aataaaggta gactagatga ttcttataag ctgggggcta ttatctataa aggccaatat
     5161 gcaaaggata atatagatga ctcatgggga aaggtcgata tctacctgat agatccaaag
     5221 cataggaagg aacggatgga tgatagagtg gacttgcaca ataatgatga gagagatata
     5281 aggctatctc ttaaggttcc agatagcgct gagggaaaat attatctaag gcttgtggga
     5341 cgttcggatg gtgcatggag gcataaagta agaaagactg ctgaggataa gatcatcttg
     5401 gagatcggaa aagatgacaa agatgatgag gacaggggga aaaaagaata tgaaaataaa
     5461 aaaagtcctt tgagtaaatt agtattccct aacaaccctg caggattgat tggatttgcc
     5521 ttttctcatc atgaagatac taagtatata actttagata gacttgaagc acattaccaa
     5581 aaagccaaaa aaaacaaaga aagtgttgag tccagccacc aacaattggc aaatgagata
     5641 agactggatg ccataagctt tccgaaggga agatatgata tcccggttaa aggagaattc
     5701 gaaggtaaag cctttgcaag agaccggttt agggataatc cctatgtaaa taataaaatg
     5761 aatatacctg aggttttttc tcaaattgat gcgctatacc gcgacataaa ttcaaaatgg
     5821 tctgactttg ataaggtagt acgggaatct gcaaagatag cagagaaaaa aggcgcctcc
     5881 attggagttt ttgacaatgg ccaatgggac ggaatcaatc ctaaaaacgc cattgagttg
     5941 gttatgtggg taaataacag gcttgaccct aagaagaggt ataagcttga ggatgaccaa
     6001 aagggtgttt taagacagtt tgcttatctt actgaaatcc tatcacttga ttgggatacc
     6061 tttgaacttg gcgtctggga aatagacaga ctgatagaaa aaatatataa gaaaaagaaa
     6121 taaagaccca atttatctat tgtaggtcgg tctttggtta agaacctgac ctcgccactt
     6181 caacagtttt ctatctaatt ttaacactaa ctcagtttga cagttgggat tttaccag
//
LOCUS       DASRUK010000062         3326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1564953, whole genome shotgun sequence.
ACCESSION   DASRUK010000062 DASRUK010000000
VERSION     DASRUK010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1564953"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VFF28_02935"
     CDS             <1..964
                     /locus_tag="VFF28_02935"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015590543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HZX44617.1"
                     /translation="VITEKQIDKDKETGSKVGTTGRGIGPCYMAKIERTGIRMVDFAK
                     QNSPEAKKLKPFVADTYVLINKAIEEQKNILIEGAQATMLDIDHGTYPFVTSSNPTAG
                     GQCVGLGIGPTKIDEVIGIQKAYTTRVGRGPFPTELGTDEDTTKEGTWDKIESIFEKQ
                     LGESAQKANAGDIYHQGKYLRLMGREYGTTTGRPRRTGWFDAVIARYARKINGFTSYV
                     LTKADCFQGLKKIKICVAYDIDGKITEDFPIDMEKLAKAKPVYEEMDGWQEDISGAKS
                     LDELPKNCLKYVKRIEQLTGVPMSILSVGPKRDETIIVNKKMLF"
     gene            1007..1366
                     /locus_tag="VFF28_02940"
     CDS             1007..1366
                     /locus_tag="VFF28_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44618.1"
                     /translation="MKENLGVAGDVFLRYRKHISALYKGNPLVRATIDKLAYAISEDI
                     PPSKGGLKGSMEEDHILAETMLSLYVLERRLHRDNEGWKGENKHRTPEQLAYDIFRAD
                     HMPLDILVRDAIQPKLC"
     gene            complement(1363..2436)
                     /locus_tag="VFF28_02945"
     CDS             complement(1363..2436)
                     /locus_tag="VFF28_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HZX44619.1"
                     /translation="MKAAVISLGSVSSQWTIEAMKQYFDSVDNIDLKKIEVDLSTNTE
                     VLYDGKPLGKYDCIYAKGSFRYAQTLRAVTGIFYPTTYMPIMAGAFSIGHDKLITQLF
                     LQKHKIAMPTTYLASSPEAAKKILDKVNYPIIFKFPAGTGGKGVMFAESFPAASSLMD
                     ALESLRQPFIIQEYIETGGEDIRAVVVGNKVVAAMKRKAVIGEKRANIHAGGEGEAIS
                     PSAQMTKMAVEAGKAIGCDICAVDLLETPKGPVVIEVNLSPGLQGITKATGINVADKI
                     ARYLYERTKSITEAGTKKGAVEIMKEINAEKNVNEQQIVTNLDFRGDRILLPKLATDM
                     SRFTDKDEFVLKVKKGRIEIEKV"
     gene            complement(2433..2792)
                     /locus_tag="VFF28_02950"
     CDS             complement(2433..2792)
                     /locus_tag="VFF28_02950"
                     /inference="COORDINATES: protein motif:HMM:NF017434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HZX44620.1"
                     /translation="MNYRQLDGKTVIGLVEPVTIITKEGAKKTILAKIDTGASKSSID
                     VNLASELKLGPIIKSKLVKSASGNKLRPVIEVRVEFAGKEMFEEFTLADRWHMKYRLL
                     VGQNLLKHGYLIDACKK"
     gene            2891..3085
                     /locus_tag="VFF28_02955"
     CDS             2891..3085
                     /locus_tag="VFF28_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44621.1"
                     /translation="MSTTIALKEDTYFLLKMVKEETKSETFDEAIQKIIAKTKSQQKS
                     YFGKFKGLGEFKREEIDRLN"
     gene            3069..>3326
                     /locus_tag="VFF28_02960"
     CDS             3069..>3326
                     /locus_tag="VFF28_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44622.1"
                     /translation="MTALIDSWGWIEYFNGTEKGKLISKYIDSSQSLFISVINLAEVY
                     KWGLLKKTENDADEMVHIMLSRCFIVPVEPETALNAAKLSRE"
BASE COUNT          970 a          661 c          737 g          958 t
ORIGIN      
        1 tgttattact gaaaagcaga tagataaaga caaggagact ggcagcaagg ttggtacgac
       61 cggcagggga atagggccgt gttatatggc aaagatagaa agaactggga ttaggatggt
      121 tgattttgca aagcaaaatt ctcctgaggc taaaaagctt aagccctttg tcgctgacac
      181 ttatgttttg atcaataaag cgattgagga gcaaaagaat atacttattg aaggcgctca
      241 agcgaccatg ttggatatag atcacggaac ttatcccttt gtcacttcat caaaccctac
      301 agcaggagga cagtgtgtag gcttggggat aggccctacc aagatcgacg aagtcatcgg
      361 gatacaaaaa gcatacacca cacgagtagg aagaggacca tttcccacag aacttgggac
      421 tgatgaagat acgaccaagg aaggaacatg ggacaagata gagtcaattt ttgaaaagca
      481 actgggtgaa tctgcgcaga aagcaaacgc aggcgatata tatcatcagg gaaaatacct
      541 caggcttatg ggcagggagt atggaacaac aacaggaagg cctagaagaa caggttggtt
      601 tgacgctgtg atcgccagat atgcaaggaa aataaacggg tttacatcat atgttttgac
      661 aaaggctgat tgcttccagg gtttaaagaa gataaagata tgcgtagctt atgatatcga
      721 cggcaagatt acagaggatt ttccgataga tatggaaaag cttgccaaag caaagccagt
      781 gtatgaagag atggatggat ggcaggaaga tatatccggg gcaaagtcac tggacgagct
      841 gccaaagaat tgccttaagt atgtaaagcg gatagagcaa ttgacaggtg ttcccatgag
      901 catactttct gtagggccga agagggatga gacgataata gtgaacaaga agatgttgtt
      961 ttagttacaa aactattatt aaatgatgga ataaaggata atgtacatga aggaaaacct
     1021 aggtgtggca ggcgatgttt ttttacgtta tcgtaaacac atatctgccc tatataaggg
     1081 taatccatta gtaagggcaa ctatcgataa attggcatat gcaatatccg aagatatacc
     1141 cccttctaaa ggtggcttga aaggaagtat ggaggaggat cacatccttg ctgaaacaat
     1201 gctttctttg tatgtccttg aacgaagatt acatagagat aatgagggtt ggaaaggcga
     1261 aaacaaacat aggactcccg aacagcttgc ttatgacata tttagagcag atcatatgcc
     1321 tctggatatc ttggtaaggg atgccataca acctaagctt tgctaaactt tttcaatctc
     1381 aattcttccc ttcttgactt tcaatacaaa ctcatcctta tctgtaaacc tggacatgtc
     1441 tgtcgcaagc tttggaagaa gtatcctgtc gcccctgaaa tcaaggttgg tgactatctg
     1501 ctgctcattc acgttctttt cagcgtttat ctctttcatt atctcgacag cgcctttttt
     1561 tgtgcctgct tcggttattg actttgtcct ttcgtaaaga tacctggcaa tcttgtcagc
     1621 aacatttata cctgtcgctt tagttatgcc ctgcaggcca ggagaaagat ttacctcgat
     1681 taccaccgga ccttttggag tttccaacag gtcaactgca catatgtcgc agccaatcgc
     1741 ttttcctgcc tcgactgcca tctttgtcat ctgggcgctt gggctaatcg cctcaccttc
     1801 gccgcctgcg tgaatatttg cccttttttc tccgatgacc gcttttcttt tcatagctgc
     1861 cacgacctta tttcctacga caactgccct gatgtcttcg cctcctgtct ctatatactc
     1921 ctggatgata aacggctgcc ttaaggattc taatgcatcc ataagtgagg atgctgcagg
     1981 aaaactttct gcaaacatca ctccttttcc gcctgtccct gctggaaact taaagattat
     2041 cggataatta accttgtcca gtattttttt tgccgcttct ggtgaagatg caagatatgt
     2101 tgtcggcatc gctatcttgt gcttttgcag gaataattga gtgataagct tgtcatggcc
     2161 gatactgaat gctcctgcca ttatcggcat gtaggtggtt ggatagaata tgcctgttac
     2221 tgcccttagg gtctgggcgt atctgaagct gcctttggca tagatgcaat catactttcc
     2281 aagaggtttg ccgtcataga ggacctcggt gtttgtcgaa aggtcgacct ctatcttttt
     2341 taggtcaata ttgtcaacgc tatcaaaata ttgcttcatt gcctctatag tccactgcga
     2401 acttaccgag cccagggaaa tgactgctgc tttcattttt tacacgcatc tataaggtat
     2461 ccatgcttta gcaggttctg gccgacaagc agcctatact tcatgtgcca tctgtctgcc
     2521 agggtaaatt cctcaaacat ctcttttcct gcaaattcga ccctgacttc tatgaccggc
     2581 cttagcttgt ttcctgatgc gctctttaca agctttgatt ttattatcgg gccaagctta
     2641 agttctgaag ctaggtttac atcgatggag cttttggagg cgcctgtgtc tatctttgca
     2701 aggatagtct tctttgcgcc ctctttggtt atgatcgtga caggctctac caagccgata
     2761 actgtctttc catcaagctg cctgtaattc atttcattta gcgtgttgga gattgttttt
     2821 aaagtttgct gttgaatatt ttagaaatat ttaaatataa gatatatcaa tataagatat
     2881 ggtgatttaa atgtcaacaa caattgccct taaagaagat acctattttt tattgaagat
     2941 ggtcaaagaa gaaacaaaaa gcgaaacatt cgatgaagca atccaaaaaa taattgcaaa
     3001 aaccaaaagc caacaaaaat cgtatttcgg caaatttaag ggtttaggtg aatttaagag
     3061 ggaagaaatt gaccgcctta attgattcct ggggatggat agagtatttt aacggtactg
     3121 aaaagggaaa gctgatatca aaatatatcg actcaagcca aagccttttt atcagcgtca
     3181 tcaacctcgc tgaagtttac aaatggggat tattaaaaaa gacagagaat gatgctgatg
     3241 aaatggttca tatcatgcta tcccgctgtt ttatcgtgcc tgtagaacct gagacagcgc
     3301 tgaatgcagc gaaattaagc agagaa
//
LOCUS       DASRUK010000063         5543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1058874, whole genome shotgun sequence.
ACCESSION   DASRUK010000063 DASRUK010000000
VERSION     DASRUK010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058874"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VFF28_02965"
     CDS             <1..433
                     /locus_tag="VFF28_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44623.1"
                     /translation="GGNAVYSLTLTNDGKESKVYTISVDSSNWADFTVSPSNVLIVGA
                     GEAKTAFINVKPNDKATAGIQVFSATIKSGESVLKEVPLRANVKGGASTSLGGLSLKR
                     ALEIGVVILIILLVILALVIGFSKLRGNDEGEEGGEEKTYY"
     gene            498..2258
                     /locus_tag="VFF28_02970"
     CDS             498..2258
                     /locus_tag="VFF28_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44624.1"
                     /translation="MKKILLIALLLFVPITSASIELGLPYFNPADYSIIKFEPYNEAY
                     VLCAGDKTVIPLLIKNENNHSDTYSFSSDSSLVTFPVQSAEISKGKSAVLPISIQPRN
                     EALNHTIKISVASKKEKIKRSVILSADILDCYNLTISPDVKQSCGDEERFELSIENNG
                     PYDRAVNVGLDAPLWVNMSGKEKMLLEAGDKIGYILAANPPESASGEFDITAYLTQNL
                     VVLATDKSTIKVNPRAECFNTEIIAPGPIQMTHEGKNVPITIKNNAVNDASYTLLVSG
                     VPWYLVSQESFSIRSKGSKVINLGLYPGEDVSFGSYKIELKAVFEGQEISKTIDVQLR
                     ESRSLLAVNGLASYYRYDIFAGIMVILAITAAFLLSMRFLPWQILKKILIYPAVIAGA
                     AVLIYLGFLSRQPLARFFSSIPLLHKPIISWNVLKYILLLAAGIIIFFIALQLASRSA
                     KTAKKKLRLANYIYAGAALLIFFSAATFIIFYGTSDHFITARDFVMGNYLYLISSFVV
                     LISVVLGFKFHGSFKAWHKYTFFAVIILAIIGMAGYFIVRYADAVKEFITLYYFYILM
                     GLGILAISILILSLTLKKES"
     gene            complement(2253..3404)
                     /locus_tag="VFF28_02975"
     CDS             complement(2253..3404)
                     /locus_tag="VFF28_02975"
                     /inference="COORDINATES: protein motif:HMM:NF015197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HZX44625.1"
                     /translation="MPKKPVLAAICTLVGFTIGAGILGIPFAVAKAGYFTGLLNIMLL
                     GLFILTLNLCLGEVTQRTKGIHQLAGYAKIYLGRKGYYLMLVVFTLFSYGALIAYIVK
                     IGEFLGAVFIPILGGNEITYSIVFFIFAFIIIYKGLSLVEKSETWMVVVMLALILIIT
                     MIALPKASIENLKGLDIKSFFVPYGVVLFAFAALPAIPSLNEELRKDRKELKKVIIIG
                     SLVPIIFYTVFATIVIAVSGLETTDGGIIGLAKYAGDKVLILGSIFGILTMATSFIAV
                     AFAMKEMFHYDYKLKERSSSTFACIIPLIVAVVLLDSGIKDLFFRILDITGTFGGSLE
                     GILVVLIWLSAKKLGKRRPEYKISCPYLISIVLILMFLLGTVFEVISWL"
     gene            complement(3749..4084)
                     /locus_tag="VFF28_02980"
     CDS             complement(3749..4084)
                     /locus_tag="VFF28_02980"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HZX44626.1"
                     /translation="MLKNISTKKVIAVDIKVCRSAFSKARGLMFSKKRALLFVFNKEK
                     KVSLHMFFVFFPIDVLYLSKNKKVVEVIKSFKPFTCYTPNHKAQYIIELPAGTIKATK
                     TKIEHVLNF"
     gene            4198..>5543
                     /locus_tag="VFF28_02985"
     CDS             4198..>5543
                     /locus_tag="VFF28_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44627.1"
                     /translation="MLILSIGEDKNHSYHSAKKEVGKMRMLKKEGMSNIRGLILFGLA
                     MVITIIYGVALFAIPAGVESGGRQVNITNVSDSNSNYLVKMNSTEYLSNTSFTVTILL
                     NKTASTDTSIELYYDVGQTPDGTANSDCKVDTNEAGQGMGPWTYTITAGGAASKCRIT
                     DGSVVAMLVQVDGINYPVEYTGAAANDTWNFTVDGAVPTLSSILIPESDDTWKTNMSL
                     IINGSDSTSTSLSYKLYINGTSNVTGTNITVGGVGTGIGLTGLMEGKYYSVLVEVSDS
                     VSNKLNSSARTFYYDATAPTTATTIGITNDTYLSSMTININVTDGGDRPLRYFVYLNG
                     SSNASGVATSGASTPVALTGLTDGQSYAIGIAALDNASNTINATVSTVFYDGTGITYS
                     NILIPATNNTWKSNMTIGFNASDVGDSSLSYKLFINNTINVTGSNLTITTALQQDGL"
BASE COUNT         1669 a         1092 c         1152 g         1630 t
ORIGIN      
        1 aggcggaaat gctgtctatt cactgaccct aaccaatgac gggaaggaat caaaggttta
       61 taccataagc gtagattcga gcaactgggc agattttacc gtcagccctt caaacgtgct
      121 gatagtcggc gcaggtgaag caaagaccgc tttcataaac gtcaagccaa acgacaaggc
      181 aacagcagga atacaggtat tctcagcaac tatcaagagc ggagagagcg tgctaaagga
      241 agttccccta agagcaaacg taaagggagg agcttctaca tcacttggcg ggctttcact
      301 taagagagca cttgagatag gtgttgtcat cctgataatc cttcttgtta tccttgccct
      361 agtaatcgga ttttccaagc ttagaggaaa cgatgaaggg gaagaaggcg gagaagagaa
      421 aacctattat tagtaaattt tttatttctt tctatatttc ttattttctg ttataaccct
      481 cctttggtga gatatcgatg aaaaaaatac tgctaatcgc acttttatta tttgttccta
      541 tcacttctgc aagcatagag cttggcctgc cttactttaa tcctgctgac tattcgataa
      601 taaagttcga gccttataat gaggcttatg ttctctgtgc gggtgataag actgtcatac
      661 ctcttcttat aaagaatgaa aacaatcatt cagatactta tagtttttct tctgatagca
      721 gcctggtcac ctttccagtg cagagcgctg agatatcaaa aggaaagagt gcagtgctgc
      781 ctatctcgat acaaccccgg aatgaggctt tgaatcacac gataaagata agcgtcgctt
      841 caaagaagga gaagatcaaa cgttctgtaa tattgagcgc ggatatcctt gactgctata
      901 atctcaccat aagccctgat gtaaagcagt cgtgcggtga tgaagagaga tttgaactta
      961 gcatcgagaa caatgggcct tatgataggg ctgtaaatgt cggccttgat gctcctttat
     1021 gggtaaatat gtctggcaaa gaaaagatgc tgcttgaagc aggcgataag ataggttata
     1081 tccttgcagc aaatcctcct gaaagtgcaa gcggggaatt tgacataact gcatatctta
     1141 ctcaaaacct tgttgtgctt gccactgata aatcgacaat taaagtaaat cccagggcag
     1201 agtgcttcaa tacggagata atcgctcctg gtcctataca aatgacgcat gaggggaaga
     1261 atgtcccaat aaccataaaa aataatgcgg tcaatgatgc aagctatact ctcttagtct
     1321 caggtgttcc ctggtatctt gtgagccaag aaagcttcag cataagaagc aaggggagca
     1381 aggtcatcaa tcttgggcta tatcctgggg aggatgtctc ttttggcagc tacaagatag
     1441 agcttaaggc tgtttttgag gggcaagaga tatctaagac aatcgatgtg cagcttaggg
     1501 agagcagatc tcttttagcg gtgaatggac ttgcaagcta ttacaggtat gatatttttg
     1561 caggtataat ggtgatactt gcaataactg ccgccttcct gttgagcatg agatttttgc
     1621 cctggcagat cttaaaaaag atattgatct atccagcggt tatagccgga gcagctgttc
     1681 ttatctatct aggattttta tccaggcagc ctttagccag gtttttttca tctatccctt
     1741 tattgcacaa gcctataata tcctggaatg tccttaaata tatcctgctt cttgccgcag
     1801 gcatcatcat cttctttatc gcgcttcagc ttgcgtcaag atccgctaaa actgcgaaga
     1861 aaaaattaag gcttgctaat tatatctatg caggggctgc actgctaata tttttttctg
     1921 ctgcgacatt tatcatcttt tatggtacta gcgatcattt tatcactgca agggattttg
     1981 tcatgggcaa ttacctatat cttatctcta gttttgtcgt gctgatatcg gtggtcctgg
     2041 gctttaagtt tcatggatct ttcaaagcct ggcataagta tacgtttttt gctgtcatca
     2101 tacttgcaat tatcggcatg gctggatatt ttatcgtcag gtatgccgat gccgtaaagg
     2161 aattcatcac tttgtattat ttctatatat tgatggggct tgggatactt gctatatcca
     2221 tattgatatt aagccttacc ctaaagaaag agtcatagcc aggaaatgac ttcaaagacc
     2281 gttccaagga ggaacataag gatcaatact atgctgataa gatacggaca gcttatcttg
     2341 tattctggtc ttctttttcc aagctttttt gcgctcaacc atataaggac aactaaaata
     2401 ccttctaaag agccgccaaa ggttccggtt atatccaata tacggaagaa aaggtccttt
     2461 atgcctgagt ccaaaaggac aacagcgacg ataaggggga tgatacatgc aaaggtggag
     2521 gaagacctct cctttagctt gtaatcataa tgaaacatct ctttcatggc aaaagcgaca
     2581 gcgataaatg aggttgccat ggtcagtata ccaaatatgg aaccgaggat aagaacctta
     2641 tcacctgcat attttgcaag gccaatgata ccgccgtctg tcgtctccaa ccctgatacg
     2701 gcgatgacta tggtcgcaaa aacggtgtaa aagataatag gaacaaggct tcctatgatt
     2761 atcactttct ttagctcctt cctatctttt cttaactcct cgtttagaga aggaattgcc
     2821 ggaagcgctg caaaggcaaa caaaacgaca ccatatggga caaagaagct ttttatgtca
     2881 aggcccttta agttttctat gcttgccttt ggcagagcga tcatcgttat aatcaagata
     2941 agggcaagca tcacaacaac catccaagtc tcactttttt ctacaagaga caaacccttg
     3001 tatatgatga tgaatgcaaa tatgaagaaa acgatagagt aagttatctc atttcctcct
     3061 agtatgggga tgaacactgc gccaaggaac tcacctatct ttactatata ggctatgagc
     3121 gctccatagg aaaacaaagt gaatacgaca agcataaggt aatatccttt cctaccaagg
     3181 tatattttcg cgtaacctgc cagctgatgt atcccttttg tcctttgcgt cacttcacca
     3241 agacagaggt ttaatgtcag gataaagagg cctaacagca taatgtttag aaggcctgtg
     3301 aagtatcctg cctttgccac ggcaaaaggt atgcccagga tacctgcgcc gatagtaaag
     3361 ccgaccaatg tgcagatggc agcaagaaca ggcttttttg gcatactcac tatttttcag
     3421 gcaagaatta tataaaggta tggggacgcc gcgactttca tctttctgat tttgaacgcg
     3481 gattagccgg tatcaccatt catcgtcttt aaagactcat cgctccaatt actggagccg
     3541 gctatactag ccaggttgta ttacgtcccc aatatagttt tgaaactatt attctttatt
     3601 taaagatgtc gataatttgt atgaattgaa cactatctcg tgaattttag cagtaaccct
     3661 ggagggagac ggtcattttc ctatcggaaa atgccctaca gggaggtttc cgacggagtt
     3721 catatctcca ccatcatcgc taaaattttc agaaattcaa tacgtgctct atctttgtct
     3781 ttgtagcctt aatagttcct gcaggaagct ctattatata ttgcgctttg tggttgggag
     3841 tatagcaagt gaatggtttg aagcttttga taacctcaac taccttcttg ttcttcgaca
     3901 gatagagaac atctatagga aaaaagacaa aaaacatatg caagctaact tttttttcct
     3961 tattgaaaac aaataaaaga gccctctttt tggaaaacat caaacctctt gcttttgaaa
     4021 aagcgctcct gcaaactttt atgtcgacag cgatgacttt ttttgtgctg atgtttttta
     4081 gcatttgtta atcaatgagg gcaatactta tataattttc tcctgtacat ctgtcttagc
     4141 gcaggcaaca caagcaatcg aaaggtttaa atatatgcgt tgtcggtttt actatcaatg
     4201 ttaatcttat ccatcggtga ggataagaat cacagttatc attcggcaaa aaaagaggtt
     4261 ggaaaaatga ggatgttgaa aaaagaaggt atgtctaaca ttagaggatt gattcttttt
     4321 ggtttggcaa tggtaataac tataatttat ggtgttgcat tatttgccat acctgcggga
     4381 gtcgaatctg gaggaaggca ggttaacata actaatgtaa gtgattccaa ctctaattat
     4441 cttgtaaaga tgaacagtac ggagtatctt tccaatactt cttttactgt cactattctg
     4501 ctgaataaaa ctgcaagtac tgatacatca attgaattat actatgatgt tgggcaaact
     4561 cctgatggaa ctgcaaacag tgattgtaag gtggatacca atgaagccgg ccaaggaatg
     4621 ggaccatgga catataccat cactgcagga ggagctgctt caaaatgcag aatcactgat
     4681 ggctcagttg ttgctatgct tgtccaagtt gatggcataa actatcctgt agagtataca
     4741 ggtgcagcag caaatgacac atggaatttt accgtggatg gggctgtccc cacactgtcc
     4801 agcattttaa tccctgaatc tgatgatacg tggaagacaa atatgtcttt gataattaat
     4861 ggatctgatt ctacctctac ttcactgtcc tacaaattat atatcaatgg aacaagcaat
     4921 gtgactggaa ccaatataac agttggtgga gttggaacag gtatcggctt aacgggtctt
     4981 atggaaggaa aatattacag cgtccttgtg gaagtttcag attcagtgag caacaaacta
     5041 aactcatctg caagaacttt ctattatgat gcgacagcac caacaacagc aacaacgatt
     5101 ggaataacca atgatactta tcttagctca atgactataa atattaacgt gactgatgga
     5161 ggagataggc ctttaaggta ttttgtatat ctcaatggat catctaatgc ttcaggagtt
     5221 gccacaagtg gagcaagtac tccagtcgcc cttacaggcc ttaccgatgg gcaatcttat
     5281 gcaataggca ttgctgctct ggataatgct tcaaatacaa taaacgcaac agtatctact
     5341 gtcttttatg atggaacagg cataacctat tcaaatatcc ttatacctgc aacgaacaac
     5401 acatggaaat caaatatgac aattgggttc aatgcatctg atgtcggtga ctcctcatta
     5461 agttataaat tatttataaa taatactatc aatgttactg gctcaaacct tacaataacc
     5521 actgcgttgc agcaggatgg gct
//
LOCUS       DASRUK010000064        11448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1059208, whole genome shotgun sequence.
ACCESSION   DASRUK010000064 DASRUK010000000
VERSION     DASRUK010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 11448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11448
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059208"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VFF28_02990"
     CDS             <1..185
                     /locus_tag="VFF28_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44628.1"
                     /translation="YQWMRQNPDSEEHCMRLKENYHIGEENYLHYILIGNIKKYPTTY
                     IVVKLIRFKVEDSLEM"
     gene            277..493
                     /locus_tag="VFF28_02995"
                     /pseudo
     CDS             277..493
                     /locus_tag="VFF28_02995"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            786..1391
                     /gene="nuoE"
                     /locus_tag="VFF28_03000"
     CDS             786..1391
                     /gene="nuoE"
                     /locus_tag="VFF28_03000"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HZX44629.1"
                     /translation="MPYLKQVTISGNNYYYLFHTIREGEKYKKVSKYLGKEKPSDSDL
                     EKLKADFLAEIKKEPKTAEEKETPPNVVALLLDLQEKKGYLSKEDMIALSKEMDIPGV
                     SIYGVATFYSQFRLTKHGKYTISICRGTACHVKNSESILEAIESLLNIKKGETTKDGK
                     FTIETVNCIGACAKAPAMMINRVVYGELTKDKVKKILSEMP"
     gene            1391..3181
                     /locus_tag="VFF28_03005"
     CDS             1391..3181
                     /locus_tag="VFF28_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HZX44630.1"
                     /translation="MEDIEKKFNLEEVRKELGKDRITVGLAICGRAAGGYPIYEALQS
                     ANLKLPVDIVGCIGMCYNEPIVTVNQGGKTSIYGKVTKDNVDKLIASIKKGEENKELL
                     VCNDLKELDFYKKQKRLVMENCGIINPFKIEHYIASGGYSGLTKALKKDRKEVLQDIK
                     NSGLRGRGGAGFSTGTKWGFIADKPGKKYLICNADEGDPGAFMNKTVVVSDPFRVIEG
                     ITIGAYATGADEGYVYARAESPLAIETMQKALETAYKHNLLGKDILGIKGFNFDLHLQ
                     KGAGAFVCGEETALIASIEGKRGMPRPRPPFPAEKGLYGKPTIINNVGTWAHVSTIMK
                     IGADEYAKIGTEKTKGTKEICLTGKIKRAGVIEVPIGATLKEIVFDIGGGAPDCTEVK
                     AVLTGGPAGGCVPVEKFDTPLDYESLQSLGSIMGSGGIVVLNNESCMVEVARYFMSFT
                     QAESCGKCTPCREGTKRLLEMLTNITRGVGTLEDIDRIKLLAEFVRDNALCGLGQNAP
                     NPVLSTLTFYRDEYEHHLIKKECPAGACTGLLKYLITAKCVGCGNCARRCPAGAITGS
                     PKQRYVIDQSKCIKCGACFEACAFKAITKG"
     gene            3199..4902
                     /locus_tag="VFF28_03010"
     CDS             3199..4902
                     /locus_tag="VFF28_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HZX44631.1"
                     /translation="MIKLKINNIDVEGKPGEMIIDVARRNGIEIPHFCWNSRMERIGA
                     CRICVVEMKGSKKLAASCCTQISPGMEINTHSPRVLEARRLNMELLLANHDLNCTICR
                     ENLNCKLQRYAEELMIRDIRFQGEKRHEQPDESSVSIRRDNNKCILCEQCVRVCNDIQ
                     TVYAIGLENRGFKTKVCPAFNLSMHDSPCVNCGQCVVACPTGALTEKSDINSVINALN
                     SGKYLVAQTAPSIRATLGECFGMSPGTPVTGKMVTALREIGFHKVFDTDLGADICIIE
                     EATEFIKRLKENKNLPMITTCCPAWIKFGEQFFFEELHHMSSCRSPQAIMASLIKTYY
                     AKKIGKDPKDMVIVDIMPCTAKKFEVSRPEFQGEADFVLTTVELAKLLKQMNVDFANL
                     KDSEFDNPLGESTGAGAIFGHTGGVMEAALRTVADWVTGKDLEKIDYKAIRGMETMKE
                     AELDLAGQKVKIAVVHTLGEARKLLNQIKQGKSRYHFIEIMACYGGCIGGGGQPPPHN
                     KKTLMKRTKALITADENKGQRKSHKNPSALKIYKEYLGEFGSEQAHKLLHTKYSKREY
                     I"
     gene            complement(5045..5530)
                     /locus_tag="VFF28_03015"
     CDS             complement(5045..5530)
                     /locus_tag="VFF28_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44632.1"
                     /translation="MDLTIKAYLERSENELILSQAILKISQENEAKSFLGIDEGLTFF
                     SSVISHSYYSIFYSAKALLLTKGIATSAPEIHKKTFDEFKRAFVDTGILDVKLFEIYK
                     KLVVRADALLDIFKDEKWKRGNFTYNTIPQANREPATDSFNNAKTFVSNITKIIESNH
                     D"
     gene            complement(5502..6134)
                     /locus_tag="VFF28_03020"
     CDS             complement(5502..6134)
                     /locus_tag="VFF28_03020"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZX44633.1"
                     /translation="MLKKEYELLIPFVQKPWEKFTFKDVKKITNKTSESYVYNGLKRF
                     VKQGLLIEERAGNVVLYAPALQRMKTQVYFGIAAEHAAWNKKHIPFDDLEKISLLIPT
                     GFYTWIITGSYAKNKQKKESDIDIVILVDDEADTKKVYAAMKFKCEMNIPPIHLYVFK
                     KEEFLEMLLNKEANYGKAIAANNLILYGASEYFGIMSEAIKNGFNDKSIS"
     gene            complement(6553..6711)
                     /locus_tag="VFF28_03025"
                     /pseudo
     CDS             complement(6553..6711)
                     /locus_tag="VFF28_03025"
                     /inference="COORDINATES: protein motif:HMM:NF037411.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6881..8689
                     /locus_tag="VFF28_03030"
     CDS             6881..8689
                     /locus_tag="VFF28_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010885206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PINc/VapC family ATPase"
                     /protein_id="HZX44634.1"
                     /translation="MANDDKLIPDTSVIIEGLLSKQLEKKEIKPQTIVIHEAVLAELE
                     HMANENKSIGYMGLNEIQSLRKLTEVNFSGKRPSSQEIKYASLGEIDSMIRQLAYDEG
                     GTLVTSDKVQREVAEARGVNVIYHSPKEASKELKLDEFFDETTMSVHLRENVTPFAKK
                     GSPGNWQFVTLRNTKLKQEEIQDIAHEILENAKLRTDGFIEIERPGSTIVQLGRYRIV
                     IAKPPFSDGWEITAVRPVKTLDLADYKLSDKLMNRIAVQAEGILVAGAPGMGKSTFAQ
                     AISVYYANQNKIVKTVEAPRDLILPEEITQYAISHGTPQEIHDILLLSRPDYTIFDEM
                     RNTDDFKLFADMRLAGVGMVGVVHATNPIDAIQRFIGRVEMGVIPQIIDTVVFIKDGQ
                     INRVLSIRMTVKVPSGMTEADLARPVVVITDFETEKLIYEIYSYGEQTVVIPVQEEIT
                     KTGVHKLAEISIENVFRQYSDKVEVKCVSDNKCIVKVPDQFIARIIGKEGKTINEIEK
                     RIGMSVDVQSMDQRQVESKDKKEVPYEVLIKKNSIMFQAGMEMQEKDIDIYVNGEFAL
                     TVKSGKTGVVKIKKTNPIGRMLIKAYNNREDIKLLA"
     gene            9069..10352
                     /gene="serS"
                     /locus_tag="VFF28_03035"
     CDS             9069..10352
                     /gene="serS"
                     /locus_tag="VFF28_03035"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HZX44635.1"
                     /translation="MLDIKIIRENPDLVRHDLKKRQDAEKLKWVDDLIEKEKEYRNLL
                     QLNQDLRHKRNVITEEINALRKQGKDFQAQVKEAKLIPEKIKEVDEHLAEIKEKLDFY
                     LMRLPNILHGSVPYGKDSSENQVVRTWGKIPEFNFELKPHGELIESLGLANFEDAAKV
                     SGKGFYYLLGDLALMEQALQRFAVDTLVKKGFTLVEPPLMLRRKPYEGVTDLADFETM
                     MYKVEGENEDLFLIATSEHPIGAMMMNKVLEQGKMPLKYVGVSPCFRKEIGSHGIDEK
                     GLFRVHQFNKIEQFVFCRPEDSWKIHEQLIENAEKIFQKLKLPYRIVNICTGDIGIVA
                     AKKYDMEAWMPREKAYKEVVSCSNCTSYQAVRLNIRYIKGAEKEYIHTLNSTAIATGR
                     ALRAILENYQQKDGSIKVPAVLQEYMGGLKVIGKK"
     gene            10391..11215
                     /locus_tag="VFF28_03040"
     CDS             10391..11215
                     /locus_tag="VFF28_03040"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZX44636.1"
                     /translation="MPSIRSFDNTKIYYVFHKGKRDTLLFVHGWPHNHTVWEKEIKHF
                     SKKGFATIALDLRGHGLSGRPKSKSSYTMESFAKDIKAIILALDIKHPILIGHSFGGM
                     ILLKFEELFPKSARCLILVDTTYENPLKDLPIIKYLNLTPFTRYLLDHILEHKHLQKK
                     LFREIDFSILKTHSDLYCWIKGAKNTSLDSLLLCLKDMLDFNERSILSKTTIPCLIIE
                     GEKDHKTPLKVAKYMQKKIPGSDLAVIRGATHDTNIRNPRQVNRVIEAFLYHQGIG"
BASE COUNT         3819 a         2187 c         2455 g         2987 t
ORIGIN      
        1 catatcaatg gatgaggcaa aatccagact ccgaagagca ctgcatgaga ttaaaggaaa
       61 actaccatat cggagaagaa aactatctgc actatatcct tattgggaat attaaaaaat
      121 accctactac ctatattgtt gtcaaattga taagatttaa ggttgaagat tctttggaaa
      181 tgtaaaatgg acaaatcaaa cgtaataaac atagtaaaaa gcctatccct aaaaaagatc
      241 agattatttc tatgaaggaa cagactttct tgaatcaggc gacatgaaag ctgctgtcag
      301 gtcattcaag aaggcgcttg aacttgatga gaaatatgta gatggccttg gcaatgttta
      361 tttctatgat gacctagaaa aggcaaagca gtattaccaa aaggtgtttg atcttgcaaa
      421 aaagcacttc aatggcaaat ggccaccaac catccagtcc agtgggcaat ccttaaaaac
      481 aggcagtagc taagagcgat gcctgggctt ggtttaatat tatggcgttt catgtggtaa
      541 ttaaaaccct cttttaaatc cattggtgat taatcaagtg gtaattaaaa aacaagtata
      601 atcttagtgg taattaataa tttattcaaa ttcaaaaata gtggtaatta aattaactct
      661 atcgtcattg tggtaataat ctaccattaa ccactataat tgtggtaatt aaatctattc
      721 agtgattttg tggtagttaa cgaaaaaaag aaaccttttt atatacgtgg tagttaaatc
      781 aattcatgcc ttatttaaag caggttacca tcagcggcaa caattattac tatctctttc
      841 acacgataag ggaaggagaa aaatacaaaa aagtaagtaa atacttaggc aaggaaaagc
      901 cgtctgattc agacctggaa aaattaaaag cagattttct tgctgagata aaaaaagagc
      961 caaagacagc agaggaaaaa gaaacaccac ccaatgttgt agcgcttctt ttggacctgc
     1021 aggaaaaaaa aggctacctc tcaaaagaag atatgatagc gctttcaaaa gagatggata
     1081 ttcctggagt aagcatctat ggcgttgcca ctttctattc tcagtttagg ctaacaaagc
     1141 acggcaaata tacgataagc atctgcaggg gaacagcctg ccatgtaaaa aattcagagt
     1201 ccatccttga agcgatagaa tcattgctta acataaagaa aggcgagacc actaaagatg
     1261 gtaagttcac gatagaaaca gttaactgta tcggagcctg tgcaaaagcg ccagctatga
     1321 tgataaacag agttgtttac ggcgagttga ctaaagataa ggtaaaaaag atcctaagcg
     1381 agatgcccta atggaagaca tagaaaaaaa gtttaatttg gaagaagtaa gaaaagagct
     1441 gggaaaagac aggattactg tcggccttgc tatctgcgga agagcagcag gcggctatcc
     1501 gatttatgaa gcattacagt ctgcaaacct aaagctccct gtagacatcg tcggctgtat
     1561 agggatgtgc tataacgagc ccatagtcac agtcaatcaa ggtggcaaaa catcgatcta
     1621 cggcaaagtc acaaaggata acgttgacaa actaattgct agcataaaaa aaggagagga
     1681 aaataaagag cttcttgtct gcaatgactt aaaggaacta gacttctaca aaaagcaaaa
     1741 aagattggta atggaaaact gtggcataat caatcccttc aagattgagc attacatagc
     1801 aagcggcggt tacagcggcc ttacaaaagc cctaaaaaag gataggaagg aagtccttca
     1861 agatataaaa aattctggcc tgaggggaag aggcggcgca ggattttcaa caggcaccaa
     1921 atggggattt attgcagaca agcctggaaa aaaatacctt atctgcaatg cagatgaagg
     1981 agaccctggt gcattcatga ataagacagt tgtcgttagc gatcccttta gggtcatcga
     2041 aggtataaca ataggcgctt atgcaacagg agcagacgaa ggttatgtct atgcaagggc
     2101 agagtctccc ctggcgatag agaccatgca aaaagcatta gagaccgcct acaaacataa
     2161 tctgctcgga aaagatatcc ttgggataaa aggatttaat ttcgatcttc atcttcaaaa
     2221 aggagcaggc gcatttgtct gcggcgaaga gacagcattg atagcatcca tcgaaggaaa
     2281 aaggggcatg ccaaggccaa ggcctccctt tccagctgaa aaaggcctct acggtaagcc
     2341 caccatcatc aacaatgttg gcacatgggc tcatgtttcg acaataatga agataggtgc
     2401 agatgaatat gcaaagattg gcacagagaa aacaaaaggc acaaaagaga tatgcctaac
     2461 aggaaagata aagcgtgcag gagtaataga agttccgata ggcgcaaccc taaaagagat
     2521 agtatttgat atcggcggtg gagcacctga ttgtacagaa gtaaaagcag ttcttacagg
     2581 aggcccagca ggcggttgtg tgccagttga aaaatttgat actcctttgg actacgagtc
     2641 gctccaaagc cttggctcta tcatgggctc tggaggaatt gttgttctta acaatgagtc
     2701 ctgcatggtt gaagtagcaa gatactttat gtcatttact caagcagaaa gctgcggcaa
     2761 atgcacgcct tgcagggaag gcacaaagcg cctcttagag atgctcacaa atatcacaag
     2821 gggcgtcggt acacttgaag acatagacag gataaagctc cttgcagagt ttgtcaggga
     2881 caacgcttta tgcggtcttg gccagaacgc tccaaatcca gttctttcaa cactcacctt
     2941 ttacagggat gaatatgaac atcacctgat aaaaaaagag tgccctgcag gtgcctgcac
     3001 cggtctatta aaatatctta tcacagcaaa gtgcgtcggc tgcggaaact gcgcaagacg
     3061 ctgccctgca ggagcaataa ccggcagtcc caaacaaaga tatgttattg accagtcaaa
     3121 atgcatcaaa tgcggcgctt gctttgaagc atgcgctttc aaagcgataa caaaaggatg
     3181 atacaaacat aattggccat gataaagcta aagattaaca acattgatgt tgaaggaaaa
     3241 ccaggagaga tgataataga cgtcgcaaga agaaacggca tcgagatccc tcatttctgc
     3301 tggaactccc gcatggaaag gataggcgca tgcaggatct gcgttgtcga gatgaaaggc
     3361 tcaaaaaagc ttgctgcctc ctgctgtact cagatatccc cgggtatgga gataaacacc
     3421 cattcaccca gagttcttga agcaagaagg ctcaacatgg agctgctcct tgcaaaccac
     3481 gatctaaatt gcactatctg ccgtgaaaac cttaattgca agcttcaaag atatgctgaa
     3541 gagctgatga taagagacat ccggtttcaa ggtgaaaaaa ggcatgagca gcctgatgag
     3601 tcaagtgttt ctatcagaag agacaataac aagtgtatct tgtgtgagca atgcgttagg
     3661 gtttgcaacg acatccagac tgtctatgcc ataggtcttg aaaaccgagg ctttaagacc
     3721 aaggtttgtc cggcattcaa cctcagcatg catgacagtc cctgtgtcaa ttgcggccag
     3781 tgtgttgttg cctgcccgac aggcgctctc acagaaaaaa gcgatatcaa ttcagttata
     3841 aatgcattaa actcgggtaa atatcttgta gcccagacag caccttcgat tagagcaacc
     3901 cttggagaat gctttggcat gtcaccagga acccctgtca caggaaagat ggtgactgca
     3961 cttagagaga taggttttca caaagtgttt gacacagacc ttggagcaga tatctgtatc
     4021 attgaggaag ccacagaatt catcaagcgc cttaaggaaa acaaaaacct gcccatgatc
     4081 acaacatgct gcccagcttg gataaagttt ggagagcagt ttttctttga agagcttcac
     4141 cacatgtctt cctgcaggag cccccaggcg atcatggcat ctttgataaa gacttattat
     4201 gcaaaaaaga taggtaaaga tccaaaagac atggtcatcg ttgacatcat gccttgcaca
     4261 gccaagaagt ttgaggtttc acggccagag ttccagggtg aggctgattt tgtactgacc
     4321 accgttgaac ttgccaaatt gttaaagcag atgaatgttg attttgctaa cctaaaggat
     4381 tcagaatttg ataatcctct cggagaaagc acaggtgcag gggcaatctt cggccataca
     4441 ggtggcgtca tggaagcagc acttaggaca gtcgctgact gggtcacagg aaaagacctg
     4501 gaaaagatag attacaaggc aatcaggggc atggagacca tgaaggaagc agaacttgac
     4561 cttgccggcc aaaaggttaa gattgcagtt gttcacaccc tgggtgaagc aagaaagctc
     4621 ctcaaccaga taaagcaggg caagagcaga tatcatttta tcgagattat ggcatgctac
     4681 ggcggctgca taggcggcgg tggccagcct cccccccata ataaaaagac attgatgaaa
     4741 aggacaaagg cgctgatcac agctgatgaa aacaaaggtc aaagaaaaag ccacaagaat
     4801 ccctcagctc taaagattta caaagaatac cttggcgagt tcggtagcga gcaggcgcac
     4861 aaactccttc atacaaaata cagcaaaagg gaatatattt aaagaaaatc ctggctaact
     4921 actctcatgg aaaaacctaa ccaagaataa gacatggtat gagttgtgtt ttaattgctc
     4981 aagacttgag cacgcagctg ggctagcgat atctcagttt gcggttcagt atcaaagagt
     5041 tctatcaatc atgattgctt tcaataatct ttgtaatatt tgaaacaaaa gtcttagcgt
     5101 tattaaaaga atctgtagct ggctctctgt ttgcctgagg aatggtgtta tatgtaaaat
     5161 tgcctctttt ccacttttca tccttgaata tatccagcag tgcatcagct cttacaacca
     5221 gctttttata aatctcaaat agtttgacat caaggattcc agtatcaaca aaagcccttt
     5281 tgaattcatc aaatgttttc ttatgtatct caggagcaga tgtagctatc ccttttgtaa
     5341 gtaataatgc tttggctgaa taaaagatag aataataaga gtgagatatt acagagctaa
     5401 aaaatgtcag cccttcatct atccctaaga agctctttgc ttcgttctct tgtgatatct
     5461 tgagtatcgc ttgagaaaga ataagctcgt tctctgacct ttcaagatat gcttttatcg
     5521 ttaaatccat tttttattgc ctcgctcata atcccaaaat attctgatgc gccatagagt
     5581 atcaggttgt tagcagcaat tgctttgcca taattagcct ctttatttag gagcatttct
     5641 aaaaattcct cttttttaaa aacatacaaa tggatcggag ggatattcat ctcgcattta
     5701 aatttcatgg cagcatacac ctttttggtg tctgcctcat catcaaccag tataactata
     5761 tcaatatctg attccttttt ctgtttgttc ttagcatagc tccctgtaat aatccaagtg
     5821 tagaatccag ttggaatcag gaggctaatc ttctccaagt catcaaaagg aatatgcttt
     5881 ttgttccatg cagcatgctc tgcagcaatt ccgaagtata cctgcgtttt catacgttgc
     5941 aatgccgggg cgtatagcac tacatttcct gctctctctt caataagtaa tccctgctta
     6001 acaaatcttt ttaggccatt ataaacatag ctttctgagg ttttattagt aatcttcttg
     6061 acatccttga aagtaaactt ttcccaaggc ttttgtacaa acggaattag cagttcatat
     6121 tcttttttta gcatttcaat ccttttttta ggtttattta atcctaaatt aaggattgtt
     6181 tataaatttt gctttttcat ataggcagca ggaagtctat aatgagatca tcctctttaa
     6241 ctccttcatt aagcaggtgg gattgtgctt ggtttatttg ttgtaaacac cccactcact
     6301 tgcggtgact gggttattgt tatggctcgc cacgttgagg gtatttacaa cactaaaaat
     6361 accccgcccc ttggggcggt tgggattttt attgctcaaa ctgggttcat accccgcagc
     6421 tcggctagtg gttgggattt tttatttgcc caaaacaagg tttccaattt tgcatccttg
     6481 gaaataacct ctggatatat acctgggtca agatggcatt cggttttatc gtctagtttg
     6541 cgccattcct tatcaaattt gattcgagca ttcaattgct tcatcataat aagtctgttt
     6601 gtcatccttt cttaaatcag agtattttgt aatgtcattt gatggtaagg atagcaattt
     6661 gccgccagga agaattgggc tgggataaca ttcaaatgca gaatcaaaac ctttacggct
     6721 taattttcat gatgataaca ctaaacaacg acagttcata acttttgccg cacgcaaagt
     6781 cctaatttgg taaactcaaa aaggattacc taccttatct attttaaatt gagtagttca
     6841 atccaaaact atttaaacat taactccaac aatcggcatt atggcaaacg acgataagct
     6901 catcccggac acatctgtga taattgaagg ccttctctca aagcagctcg aaaaaaagga
     6961 gataaagcct caaaccatag tcatccatga agcagtcctg gcagagcttg agcacatggc
     7021 aaatgaaaat aagtcgatag gttatatggg attaaacgag atccaatcat taaggaaact
     7081 cacagaagta aacttctcag gcaaaaggcc ttcttcgcag gaaataaagt atgcgagctt
     7141 aggcgagata gacagcatga tacgtcagct tgcatatgat gaaggcggaa cgcttgtaac
     7201 ctcagacaag gtccaaaggg aagtggcaga agcaagaggt gtaaatgtta tttatcacag
     7261 tccaaaagaa gcaagcaagg agctaaagct tgatgaattc tttgatgaga caactatgtc
     7321 tgttcactta agggagaatg ttacaccttt tgcaaaaaaa ggatctcctg gaaattggca
     7381 attcgtaact ctaaggaata ctaaactaaa acaggaagag atacaggata ttgcacatga
     7441 gattcttgaa aacgcaaaac taaggacaga cggttttata gagattgaaa ggccaggctc
     7501 aacaatcgtc cagcttggaa gatacaggat agttatcgca aagccgccat tctcggatgg
     7561 ctgggagata acagcagtaa ggccggtaaa gacccttgat ctagccgatt acaagctaag
     7621 tgataagttg atgaacagga ttgcagtgca ggcagaagga atccttgttg caggtgcgcc
     7681 aggcatggga aaaagcacat ttgctcaggc aatatcagta tactacgcaa atcaaaataa
     7741 gattgtaaag acagtagaag ccccaagaga tcttatcctg ccagaagaga ttacccagta
     7801 tgctatctct catggaaccc cgcaggagat tcacgatatc ctgttgttaa gcaggcctga
     7861 ctatactatt tttgacgaga tgagaaacac cgatgatttc aagctctttg cagatatgag
     7921 gcttgcaggt gtaggcatgg tcggagttgt ccatgcgaca aacccgatag atgctatcca
     7981 aagattcata ggcagggttg agatgggagt catccctcag ataattgaca cagtcgtctt
     8041 cataaaagac ggccagatca accgagttct aagtataagg atgaccgtaa aagtcccttc
     8101 aggcatgaca gaagcagacc tcgcaaggcc agtagtggtt attacagact ttgagacaga
     8161 aaaactgatc tacgagatat actcttatgg cgaacagacg gttgtaatcc ctgtccagga
     8221 agagataaca aagaccggag tgcataagct tgcagaaatc agtattgaaa atgtattcag
     8281 gcaatactca gacaaggtag aggtaaaatg cgtctcagac aacaaatgca ttgtaaaagt
     8341 ccctgaccag ttcatcgcaa ggataattgg aaaggaagga aagacgatca atgagataga
     8401 aaaaaggata ggcatgagtg tcgacgtcca gtctatggac caaaggcaag tggaatcaaa
     8461 agacaaaaaa gaagtccctt acgaagtcct cataaagaag aattccataa tgttccaggc
     8521 aggcatggag atgcaggaaa aagacataga tatctatgtc aacggtgaat ttgcccttac
     8581 agtaaagtca ggaaagacag gcgtagtaaa gataaagaag acaaatccca tcggaaggat
     8641 gctgattaag gcgtataata atagagaaga tattaagctg ttggcttagc taacaatgga
     8701 tgtgaagatc atatggatat tataaaatta gcaagcaaag gaagattgaa taagatagta
     8761 attaagacct taccttttaa taggtcattg actaaatttg gtatttattt tatgttaata
     8821 ggatacctat taggcagtat catgtttctt attataggcc caaaaattat ggcgttgata
     8881 tacctcattt ttggttattc aatggttgta tttctaatga ttcctagtta ttttgctttg
     8941 gattacactt gcaaaaatta tcttgggaaa gggagggtaa agtaaatttt tgtccaaatt
     9001 atacctagac aacttaacag ctcatcaaat aacaaccttt ttataattct ggataacatt
     9061 caatggatat gctggatatt aagataatca gggaaaatcc tgacctggtt aggcacgact
     9121 tgaagaagcg ccaggatgca gaaaaactaa agtgggtaga cgatcttata gaaaaggaaa
     9181 aggaatacag aaaccttctt cagctcaatc aggatctaag gcataaaaga aatgtcatca
     9241 cagaagagat taacgcttta agaaagcagg gcaaggattt tcaagcccag gtaaaggaag
     9301 caaagcttat cccagaaaag ataaaggagg tagatgagca tctagcagaa ataaaagaaa
     9361 agcttgattt ctaccttatg cgccttccaa acatccttca cggatcagtc ccttacggaa
     9421 aggattcaag cgaaaaccag gttgtaagga catggggcaa gatacctgaa ttcaactttg
     9481 agctaaaacc tcacggcgaa ttgatagaaa gtctaggcct tgcaaacttt gaggatgcag
     9541 ccaaagtttc aggaaaaggg ttctattatc tgttgggaga tttagcattg atggagcaag
     9601 ccctccagag atttgctgtt gacacactgg taaaaaaagg atttaccctt gtagagccgc
     9661 ccttgatgct aagaagaaag ccttatgaag gcgtcacaga cctagcagac ttcgaaacca
     9721 tgatgtacaa agtagaggga gaaaacgaag atttgttcct tatagcgaca agcgagcacc
     9781 ctatcggcgc catgatgatg aacaaggtgc tcgagcaggg caagatgcca cttaagtatg
     9841 tcggtgtaag cccttgcttc agaaaagaga taggctctca tggcatagat gaaaaaggtc
     9901 tattccgagt ccaccaattc aacaagattg agcagttcgt tttttgcagg ccagaagatt
     9961 cttggaagat tcatgagcag ctcatagaaa acgcagaaaa gattttccaa aagctaaaac
    10021 tgccctatag gatagttaac atctgcacag gcgatatagg cattgtggca gcaaagaaat
    10081 atgacatgga agcttggatg ccaagagaaa aagcatacaa ggaagtggta agctgctcaa
    10141 actgcacctc ctatcaggca gtaaggctta acataagata tataaaagga gcagaaaaag
    10201 aatatatcca cacattaaac tcaacagcca tcgctacagg aagagcatta agggcgattc
    10261 tcgaaaacta tcagcaaaaa gatggctcga taaaagtccc ggcagtcctc caggaataca
    10321 tgggagggct aaaggttatt gggaaaaaat agcctaatgt tttatatatt cttcttattc
    10381 agtaatagtt atgccctcaa ttagatcatt tgacaatacc aaaatatatt atgtcttcca
    10441 taagggtaaa agagataccc tccttttcgt ccacggatgg cctcataacc atacagtatg
    10501 ggaaaaagag attaagcatt tttcaaaaaa aggttttgct acgatagcac ttgatttgag
    10561 agggcatgga ttgtcgggca ggccaaagtc caaatcctca tataccatgg aaagtttcgc
    10621 aaaagatatc aaagcgatta tcctcgctct tgatattaaa catcccattc ttatagggca
    10681 ttcttttgga gggatgatct tgcttaaatt cgaggaatta tttccaaaaa gtgcaaggtg
    10741 tctgatattg gtcgatacta cctatgagaa tccactaaaa gatctaccaa taattaagta
    10801 tctaaatttg actcccttca caagatatct gcttgatcat atcttggagc ataagcatct
    10861 acaaaaaaag ctgttcaggg agatagactt ttccattttg aagactcatt ctgatttata
    10921 ttgctggatt aaaggagcaa agaatacttc tctggatagc ttgttattat gtcttaagga
    10981 tatgctggat tttaacgaga gaagcatctt aagtaagaca acaatccctt gcttgataat
    11041 agaaggggaa aaagaccata aaactcccct gaaagtagca aagtacatgc aaaagaagat
    11101 tccaggctct gatttggccg tgataagggg tgctacccat gacaccaaca ttagaaaccc
    11161 aagacaggta aatagggtta ttgaggcttt tttatatcat caaggaattg gttgatctct
    11221 ctggctgcta taccaggatt aaagatcggc cagtcatgat tcactccatt gacaattttg
    11281 accttgccgt tgggtgccat ctcttcaatt aactacccca agtcttaaat cttgtttcgt
    11341 tttcgaaata gttgcttatc ctgctcattg cttctctaac ggtttggaaa ttgctgtttt
    11401 gtaagatgtc tttgtctaat tttgagaaaa ctctttcaat catattca
//
LOCUS       DASRUK010000065         5493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_220586, whole genome shotgun sequence.
ACCESSION   DASRUK010000065 DASRUK010000000
VERSION     DASRUK010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220586"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..809)
                     /locus_tag="VFF28_03045"
     CDS             complement(102..809)
                     /locus_tag="VFF28_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44637.1"
                     /translation="MKKYMLGLVVLLFLFGCEQLQGETATPGIDVRDQIVLDTVQVDL
                     VSSNSTGWVAIYNERDGQPDQLIGFTQVEAGENRNITVNITQNRITSTMYAVLHNDRG
                     EEGRLEFPGPDAPVMVDGSFVAQTFRALGEATDETAQNDTEDTANDTITPVPSTISSD
                     NETEGAGEDDNETEDIATGNETADDTTEDETADETTDDETDEAIDNTSENNVLEYSSG
                     DDGLEMIARKISSAVSY"
     gene            926..1141
                     /locus_tag="VFF28_03050"
     CDS             926..1141
                     /locus_tag="VFF28_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44638.1"
                     /translation="MGQQEVYDLLKRYQSKWLNAREISKLLDASFNTVVGNLKRLRKA
                     GFVLVRKAYQVVEPAGRRLVYLYKFKK"
     gene            1152..1733
                     /locus_tag="VFF28_03055"
     CDS             1152..1733
                     /locus_tag="VFF28_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HZX44639.1"
                     /translation="MEHCDIQFRTIYKDRRQIENPLVDELMAWCIQLDRTGFAPECAG
                     NLSVRTKQGFIITTFKSDFSSMSKDDFSEVLSVDMQKKQAYANGSKDPSSETFLHHAI
                     YSARKDANAVFHAHCDAFLEQNDKLNLPITAEEKPAGSLELVDEVLKILDGNNFILLK
                     NHGFAAIGDSIGSAGNLVMKRYNQLERIKRSIS"
     gene            complement(1722..2636)
                     /gene="truD"
                     /locus_tag="VFF28_03060"
     CDS             complement(1722..2636)
                     /gene="truD"
                     /locus_tag="VFF28_03060"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HZX44640.1"
                     /translation="MYKLKENPEDFIVKEIANHNLTSQGKYAVCLLKKKEYNTMKAVQ
                     RIADTLKIPVKDIGFAGTKDKKAITEQYISIKGIKKDIIQQIKLGDIELTFKGYSDKP
                     ISLGDLEGNIFTITIRGITKIKNKKIMPNFFGEQRFSKSNVEIGRLIIKNDYKKALEL
                     IKKTDQKTSINHPNDVIGSLRSIPFKLLRLYVYAFQSHIWNEALKKYMKVSDKNIEIP
                     IIGFDTEIKDKAVKNILSRLMAKERITFRDFINRQIPELSMEGNSRRAYVEIKDLRVI
                     NKGNDFITIHFALPKGSYATEAIKVLAD"
     gene            2763..3308
                     /locus_tag="VFF28_03065"
     CDS             2763..3308
                     /locus_tag="VFF28_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44641.1"
                     /translation="MNPDKLRITPCGQEYAGGIRKNLIKVYAHPDVMAIYTKYGFDLE
                     VTWRFNREGRIITLDTRVRDEMEKPLIDVGTQPSLHWNTAKASYDQMPCQTENLNPGF
                     QYMELRKQYEALLRFGHGTATKILAELRADAIGEVIDIRPSQRQLSEIVKNQEAMGIL
                     ANISTGMPRVQMADNHLIITY"
     gene            complement(3310..4101)
                     /gene="mtnP"
                     /locus_tag="VFF28_03070"
     CDS             complement(3310..4101)
                     /gene="mtnP"
                     /locus_tag="VFF28_03070"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HZX44642.1"
                     /translation="MMKIGIIGGSGLEDPKILKDSKEIEVVTKFGKPSSPLKIGKIGG
                     IDVVILSRHGKKHSIHPGKVNYRANIMALKEQGCTHIIASSACGSLREDIKPGDFIIV
                     DQFIDRTYGRQSTFYDEDNVAHIPMAEPFCKDLRCQLCEAGRELGLNVHEKGTVVTIQ
                     GPRFSTRAESNMFRQWKGDVINMSTVPECVLAREAGICYAVVCMATDYDCWKGSEEGV
                     DIQMVLKTFKQNAENVIKLFLRTIPQIKENKECRCRTDIKSAIIS"
     gene            complement(4107..4628)
                     /locus_tag="VFF28_03075"
     CDS             complement(4107..4628)
                     /locus_tag="VFF28_03075"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HZX44643.1"
                     /translation="MNLKDKIRTVPHWPIEGVMFRDITTLLQDGEAFRQACDELYDRY
                     KNEKIDVVVGLEARGFIFGSVLAYKLGVGFVPIRKKGKLPSKTTSHTYQKEYGPDTME
                     IHTDSIKKGDRVLVVDDLLATGGTCSAANELIEKLGGHIIECAFVIDLPELKGKEKLK
                     GYKIFTLMEFEGE"
     gene            4724..5071
                     /locus_tag="VFF28_03080"
     CDS             4724..5071
                     /locus_tag="VFF28_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44644.1"
                     /translation="MPISDRLYSDRQGYLGSIYGIFIEFYNDNIVRASIRQGDKRDVV
                     LIPQAHAFKRARLDIGDTFECQLYGHNNKVYGCVVRPTEPREDEMPGKIPNGMEHLLS
                     RLEEKDTLLQSRR"
     gene            complement(5147..>5493)
                     /locus_tag="VFF28_03085"
     CDS             complement(5147..>5493)
                     /locus_tag="VFF28_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44645.1"
                     /translation="KNTSIFSYGMRIRLPDHLKDDPLVNAVATVADYIGRFEVAAWLE
                     GACKGNPNIGKPGIGRFDMANLAAESALEDRAFTGAMDVLVAHSSDPTINGILGRVYS
                     LVPKVQEHYQKP"
BASE COUNT         1520 a         1184 c         1085 g         1704 t
ORIGIN      
        1 ggcgagccat aacaataacc cagtcaccgc aagtgagtgg ggtatttaca acaaataaat
       61 gcccaagcga cgcccaggca agatttaatt gctgccagca cctaatagct tacagcgctg
      121 cttatctttc gcgcaatcat ctccaggccg tcgtctcctg aggaatattc caggacattg
      181 ttttcgctcg tgttatctat cgcttcatct gtctcatcat ctgtggtctc gtctgctgtt
      241 tcatcctcgg tcgtatcatc ggctgtctca ttgccagtcg ctatatcttc cgtctcattg
      301 tcatcttcac cggcaccctc agtctcgttg tcgctggaga tagttgatgg aactggagtt
      361 atcgtgtcat ttgccgtatc ttcagtatcg ttttgggcag tctcgtctgt tgcttcaccc
      421 agcgctctga acgtttgagc tacaaacgag ccgtctacca tcaccggagc atccgggcct
      481 gggaattcaa gcctgccctc ttcgcctcta tcattgtgca ggacggcata catcgtgctt
      541 gtaatcctgt tctgtgttat attaacggtg atatttcggt tttcgcctgc ctctacctga
      601 gtaaatccaa taagctgatc aggctgcccg tctctctcgt tatatatagc tacccatcct
      661 gtgctgttgg atgataccaa atcgacctgc acagtgtcga gcactatctg gtccctaaca
      721 tctatcccgg gtgtcgccgt ctcaccctgc agctgctcgc agccgaacaa gaatagcaat
      781 accactaagc ctagcatata ttttttcatc aaatcacccc caataaaaat agaatgaaag
      841 tatgctaaga cttgcagttt taaataattt tcgcaagcga aaatagaaat gtttatataa
      901 agtctaagat aaaaaattct aaacaatggg ccaacaggaa gtttacgatc tactaaaaag
      961 ataccagagt aaatggctta atgcacgaga gatatcaaag cttctcgatg cttcttttaa
     1021 tactgtcgta ggaaacctca aaaggcttag gaaagcagga tttgtccttg taagaaaagc
     1081 ttatcaggtt gtcgagcctg caggaaggcg cctggtctat ctatacaaat ttaaaaaata
     1141 gaaggtaaga catggagcat tgcgatatcc agtttaggac catatacaaa gaccgccggc
     1201 agatagaaaa cccgcttgta gatgagctta tggcatggtg catccagctt gaccgtacag
     1261 gctttgcacc agaatgtgct ggaaacctta gcgtgcgtac aaagcaggga tttatcatca
     1321 ctacctttaa atctgatttt tcaagcatgt caaaggacga tttttctgaa gtattgtccg
     1381 tcgacatgca aaaaaagcaa gcttatgcaa acggcagcaa ggacccctcc agcgagacat
     1441 tccttcacca tgccatctac tctgcaagaa aagatgcaaa tgcagtcttt catgcgcact
     1501 gtgatgcctt tctcgagcag aacgacaagt tgaaccttcc tatcacagca gaagaaaaac
     1561 ctgcaggttc tcttgagctc gtcgatgagg tattgaagat attagatggg aacaatttta
     1621 tccttctgaa gaaccatggc tttgcagcta tcggcgattc tataggctct gctggaaatc
     1681 ttgtgatgaa acggtataac cagctcgaaa gaatcaagag atcaatcagc taatactttt
     1741 atcgcttctg ttgcataact gccttttggt aaggcaaaat ggatcgtgat gaaatcattc
     1801 cctttattga taactcgaag gtcttttatc tcaacatacg ctcgtcttga attgccttcc
     1861 atgctgagtt caggtatctg gcggttgatg aaatccctaa atgttatcct ttcctttgcc
     1921 atcaatctcg acaagatatt cttaacagcc ttatctttta tctcagtgtc gaaccctatt
     1981 ataggtatct ctatattttt atcgctcact ttcatatatt ttttcaatgc ctcattccag
     2041 atatggcttt gaaatgcata cacatacaat ctcagaagct taaaagggat acttctcagt
     2101 gaaccgatga catcgtttgg atggtttatg cttgtttttt ggtcagtttt ttttataagc
     2161 tctaaagctt ttttatagtc attttttata atcaacctgc ctatctcaac attgcttttt
     2221 gaaaaccttt gctcgccgaa gaagttaggc atgatctttt tgttttttat ttttgttatg
     2281 cctcttatag ttatggtgaa aatattgccc tcaaggtcgc caagggagat aggcttgtca
     2341 gaatatccct tgaaggtcag ctcaatgtca cccagtttga tctgctggat aatgtctttt
     2401 tttatcccct ttatggagat atactgctct gttattgctt tcttatcctt tgtccctgca
     2461 aatccaatat ccttaactgg gatttttaga gtgtcagcta tcctttggac agctttcatg
     2521 gtgttgtatt ctttcttttt caggagacag acagcatatt taccctggct tgttaaattg
     2581 tggtttgcta tctctttgac gataaaatct tctggatttt ccttgagttt atacacagca
     2641 agcaagggca gttgaccctt taaataattt gttcaatgaa actatcctaa aatggaaaaa
     2701 tcaattcacc tgaaaatcta attggaaatc attaaaaatc aaaaatgcta ctaagacaaa
     2761 cgatgaatcc agacaaattg agaataactc cttgtgggca ggaatatgct ggaggcataa
     2821 gaaaaaatct tatcaaggta tatgcgcatc ctgatgttat ggcaatatat acaaaatatg
     2881 gttttgacct tgaggtgacc tggagattta atagagaagg aaggataatc actctagata
     2941 ccagggttcg tgatgagatg gaaaaaccat tgatagatgt aggaacacag ccttcccttc
     3001 attggaatac tgcaaaagcg tcatatgatc agatgccatg ccagactgaa aacttaaatc
     3061 cgggttttca atatatggaa ctaaggaagc aatatgaagc cttgttaagg tttggccatg
     3121 gcacagcaac caagatcctt gcggaattaa gggcagacgc aataggagag gtaatcgata
     3181 ttaggccttc tcaaaggcaa ttatccgaga tagttaagaa tcaggaggca atggggatac
     3241 ttgcgaatat atcaacaggg atgccaaggg tccaaatggc agataaccac ctgataataa
     3301 cctattgaac taactgataa ttgcactttt aatatcagtc ctgcacctac attctttgtt
     3361 ctcttttatc tgaggtatag ttcttaggaa aagcttgata acgttctctg cattttgttt
     3421 gaatgtcttt agcaccatct ggatgtcaac gccttcttct gatcctttcc agcagtcata
     3481 atctgtagcc atacaaacaa ctgcatagca tatgcctgct tctcttgcaa ggacacattc
     3541 aggaacagtt gacatgttga taacatcgcc tttccattgc ctgaacatat ttgactcagc
     3601 ccttgtcgag aatctcgggc cttgtatcgt cacaacagtc cctttttcat ggacatttag
     3661 gccaagctct cttcctgctt cgcacaattg gcatcttaaa tccttgcaga aaggctctgc
     3721 catcggaata tgtgcaacat tgtcttcatc gtaaaaggtg ctttgcctgc cataagtcct
     3781 gtctatgaat tggtcgacga tgatgaagtc gccgggcttt atgtcttccc ttaaactgcc
     3841 gcaggcagag gatgcaataa tgtgagtaca gccttgttct tttagcgcca ttatatttgc
     3901 tctgtaattt acctttccag ggtgtattga gtgctttttg ccgtgccttg aaaggattac
     3961 aacgtctatt ccgccaatct tgcctatctt tagcggtgag gagggtttgc cgaattttgt
     4021 taccacctct atctctttgc tgtcttttaa tatcttgggg tcttcaagac cgctgccgcc
     4081 gattattcct atcttcatca tatcgctcac tctccttcaa attccataag cgtgaatatc
     4141 ttgtagccct ttagcttttc cttgccttta agttcaggaa ggtctatcac aaaagcgcat
     4201 tcaatgatat ggccgccaag tttttctatc aattcgtttg cagcagagca tgtcccgcct
     4261 gtcgcaagaa ggtcgtcaac aacaaggacc ctatcgcctt tttttatgga atctgtatgt
     4321 atctccattg tatcagggcc atactccttt tgataagtgt gagaggttgt ctttgatgga
     4381 agctttcctt tttttcttat cggcacaaat ccgacgccaa gcttgtatgc aagcacagag
     4441 ccaaagatga atcctcttgc ttcaagaccg acgactacat ctatcttctc gtttttatag
     4501 cggtcgtata gttcatcgca agcctgcctg aacgcttctc catcctggag aagagtcgtg
     4561 atgtccctaa acataactcc ttcaataggc caatgtggaa cagtcctaat cttatcttta
     4621 aggttcatga tggttcaccc cttgatttat aagagaaagg ttgtataaaa tgcttgcgat
     4681 ttttccagat agttttttat atggtgctta attctccata gttatgccga taagtgatcg
     4741 attgtatagt gatagacaag gatatcttgg gagcatctat ggtatattta ttgaatttta
     4801 taatgataat atagtaagag cctcgataag acaaggcgat aaaagagatg tagttcttat
     4861 cccacaagca catgcattta agagagcccg acttgatata ggagatacat tcgaatgcca
     4921 actctatggg cataataaca aggtgtacgg ttgtgttgtt agacctactg agccaaggga
     4981 agatgaaatg cctggcaaaa ttccaaatgg gatggaacat cttctcagca gactggaaga
     5041 aaaggatact cttcttcaga gtagacgcta agtattattc taagtcctaa aatttatgaa
     5101 tttgcggatg attggcgtta aactcaattt tttgtcggtg aacctgtcag ggtttttgat
     5161 aatgctcttg cacttttggc acaagtgagt atactctacc tagaattcca tttatagtag
     5221 gatcagaaga atgagctact aaaacatcca tcgcacctgt aaatgctcta tcttctaacg
     5281 cactttcggc ggccaaattt gccatgtcaa atctaccaat accaggttta cctatattag
     5341 gattaccttt acaggcgcct tcaagccatg ctgcaacttc aaaacggcca atatagtctg
     5401 caactgtagc cacagcatta actaatggat catcttttaa atgatctggc aatcgaattc
     5461 tcatgccata tgagaatatg gaggtatttt taa
//
LOCUS       DASRUK010000066         7059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1887502, whole genome shotgun sequence.
ACCESSION   DASRUK010000066 DASRUK010000000
VERSION     DASRUK010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 7059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7059
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1887502"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1298
                     /locus_tag="VFF28_03090"
     CDS             <1..1298
                     /locus_tag="VFF28_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014122211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZX44646.1"
                     /translation="LIQPMSKDKLSKKTLELRIQKFLKDHPDKNDHFRNIDIDKLYGY
                     VQSVLTNPNLIDLEETANITYGFVGADLASLAKESAMIVLRRVLPDLKLKEEEAISKD
                     LLESLMITQKDFREALKVVRPSALREVLIEKPTTRWVDIGGLTEAKQELIEAVEWPLE
                     FPDSFRRMGIRPPRGILLYGAPGTGKTLMARAVANESNANFISVKGPELLSKWVGESE
                     KAVREVFKKARQTAPTIIFFDEVDSLAPKRGANSGSHVTESVVNQLLTEMDGLEEMQD
                     VVVIAASNRPDIIDTSLLRPGRFDRIILVPVPDEKTRLEIFKVHTKGMPIVTEDDVKK
                     GKKELVPEDAIDLNKLAKLTEGYVGADIEAVCREAAILALRDNMEAKRIIAKYFDKAL
                     NKVGPSSTKEVEKAYAELGKKFSAAKGKQLKEEMPSYFG"
     gene            complement(1525..1869)
                     /locus_tag="VFF28_03095"
     CDS             complement(1525..1869)
                     /locus_tag="VFF28_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44647.1"
                     /translation="MSKSVLSLVLAKIAGFLFILVLLGIASYFSRTVSNPDGSLEFFN
                     SNLGLFAITALLLFFAELFLTFRYPANLLYPLFNAAASIYFISFFMGLLIDNFTDAGF
                     STIVILQPFFRH"
     gene            1944..2621
                     /locus_tag="VFF28_03100"
     CDS             1944..2621
                     /locus_tag="VFF28_03100"
                     /inference="COORDINATES: protein motif:HMM:NF045198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HZX44648.1"
                     /translation="MEIFKIFRKKPVEEFISFEVVEKVSGDYTEFERKLTKQSQIILI
                     TGRRGSGKTALGMKILELFCSKSQRKCYALGFLDAKLPWKIKKIDDIEGVKLNSVVLI
                     DEGAITFSARDSMKERNKMLGKMMAIARHKNLSLILIVQNSAMIDLNVLRLADCLLLK
                     EPSLLQKDFERGPIKKIYSSVIPHFEKTADKKSHFFVWDDDFRGMLKYSLPAFWSEQI
                     SKSFKNF"
     gene            2977..3318
                     /locus_tag="VFF28_03105"
     CDS             2977..3318
                     /locus_tag="VFF28_03105"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZX44649.1"
                     /translation="MRMLWRRKKEEEKVILLPCFMAGKINASHILVKTESEANIVLYD
                     VTHGKAFEGVAREKSLCPSGKKGGELGWFGRNQMVREFEVAAFLLKKREISRPVKTQF
                     GWHIIKVNDVA"
     gene            3311..4603
                     /gene="gatD"
                     /locus_tag="VFF28_03110"
     CDS             3311..4603
                     /gene="gatD"
                     /locus_tag="VFF28_03110"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003217.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HZX44650.1"
                     /translation="MHKAGDRVKIHLKDEIVEGIFMPNEETDSIVIKLDNGYTIGIDK
                     KNVKKIEKIGEAKKKEEAVKKIKTNPSLPKITILHTGGTIASKVDYKTGAVFTSFKPE
                     DLLGMFPELSLIANFESKLLANMWSDDLRFKHFELIARAIEKEVKAGVEGIIVGMGTD
                     NLAVASAALAFIIESTPVPILFVGAQRSSDRGSSDAAMNLICAAEFIAKSDFAGVGIC
                     MHENMDDKISAIFPPCKTRKLHSSRRDAFKTVNSTVIARINYDTRNIDYTHDYTRKDK
                     KRDLAVKPNMEEKVGLLKVHVNMFPEQFSFFEGYKGLVIEGTGLGHIPGQAPDDISEI
                     HKKIYPAVKKVIDSGCIVVMTTQCIYGRVQLHVYSKGTDEVKLGIISGEDMLAETALV
                     KLSWLLGNHKDKKEIEKLMVINLRGEINKRIEPSEYLE"
     gene            4636..4881
                     /locus_tag="VFF28_03115"
     CDS             4636..4881
                     /locus_tag="VFF28_03115"
                     /inference="COORDINATES: protein motif:HMM:NF016352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF504 domain-containing protein"
                     /protein_id="HZX44651.1"
                     /translation="MISAKDYINKIKWSPGENSTEYSIGYWDNIEKKIIFIDFNEIKT
                     VVGNFLSLDRAKETYIPMHRIREIRKKGKVVWKRTTA"
     gene            4914..6305
                     /gene="pyk"
                     /locus_tag="VFF28_03120"
     CDS             4914..6305
                     /gene="pyk"
                     /locus_tag="VFF28_03120"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HZX44652.1"
                     /translation="MIRTKIVCTIGPCSETVDILSKLAKAGMNIARLNFSHGNHDEHL
                     ARIKRINEANESLPIPVGIMLDTQGPEIRLGTFEKKENLVEGEEVVITPRDVVCTSKV
                     ISISYKKIGNLVKPGDYIYIADGTIELKVKGKVDSDVVCEVVIGGEVNTRKNVAVPGA
                     IVDLPSISNKDKEDIKFGIRNGIDFVAQSFVKSEDDVYDMKKLLAGTDILTIAKIEDP
                     IGLKNIDEIIGAADGIMVARGDLGVQIPIQEVPSAQKMIIRKCNEAGKPVIVATQMLE
                     SMTRNPRPTRAEVTDVANAISDGADAIMLSGETAIGKYPLRAVEMMTRIAKETEGRIG
                     PDFSVFHDSDKVEDALSKAVCHTAYNIKANAIITCTLSGHTARMISRYKPQTPIVAVT
                     PNLKEIRKLNLWWGVHPLLIEAPSTTDDLIQNSVDIVMKKGLVKKGDIVVLTAGIPFT
                     TPGNMNLMKIHEI"
     gene            6669..6917
                     /locus_tag="VFF28_03125"
     CDS             6669..6917
                     /locus_tag="VFF28_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44653.1"
                     /translation="MVTMGGVPFFAFYPGTRQRMNALYLRMIHAPEEYQDSGKMTASR
                     GRIETLKEALLDKYRQVHLSSQPQERDSIALARIQDTL"
BASE COUNT         2291 a         1329 c         1667 g         1772 t
ORIGIN      
        1 atctgatcca acctatgagc aaggacaagc tgtccaagaa aaccctcgaa ctaaggatac
       61 agaaattctt aaaggaccat cctgataaaa atgaccattt caggaatata gacatcgata
      121 agctatacgg atatgtccag agtgtcttga caaatcctaa tcttattgac cttgaagaaa
      181 ctgcaaatat cacatatggc tttgtcggcg ctgaccttgc ttctcttgcc aaggaatctg
      241 cgatgatcgt gcttaggcga gtccttcctg atcttaaact taaggaagag gaggctatct
      301 caaaagacct tttggaaagc ttgatgataa cacaaaagga ttttagggaa gcacttaagg
      361 ttgtaaggcc ttctgcactg cgcgaggtct tgatagaaaa acctactacc aggtgggttg
      421 atatcggcgg gcttactgaa gcaaaacagg agcttataga ggctgttgag tggccgctag
      481 aattccctga cagcttcaga aggatgggga ttcggccgcc aagaggcatc cttctttatg
      541 gcgcccccgg aacaggaaag acgctcatgg cacgggctgt cgcaaatgaa tccaatgcta
      601 atttcatatc tgtcaaaggg cctgaactgc tcagcaaatg ggtaggcgaa tctgaaaaag
      661 ctgtcagaga ggtatttaag aaagcacgcc agacagcacc aactataata ttttttgatg
      721 aggttgactc actcgcccca aagaggggag caaattccgg aagccatgtg actgaatctg
      781 tggtaaatca actgcttaca gagatggacg gcctggaaga gatgcaggat gttgtggtta
      841 tcgctgcttc aaacaggccg gatatcatcg acacttcact tcttaggcca ggaaggtttg
      901 acaggataat acttgttcca gtgcctgatg agaagacaag gctggagata ttcaaggtac
      961 acacaaaagg catgcctatt gttacagaag atgatgtaaa aaaaggaaag aaggaacttg
     1021 ttcctgaaga tgccattgac ctaaacaaac ttgcaaagct aactgaagga tatgtcggcg
     1081 ctgatatcga ggctgtctgc agagaggctg caatacttgc gcttcgcgac aatatggaag
     1141 caaagagaat aatcgcaaaa tattttgata aagcgctcaa caaagtcggg ccttcatcga
     1201 caaaggaagt ggagaaagca tatgcagagc ttggcaagaa attttctgct gcgaagggaa
     1261 aacagctaaa agaagagatg cccagttact ttggataaag gccatagtta acgtgaattt
     1321 acaacttgcc tatgctcatt aaatctagag ggccagcgat atttgcccag atttagcttt
     1381 gcctaaactt ttttctaatc catcttgcga agtcatatgc agccaactgg aactgcactg
     1441 ttatatctga ccatacaatg ccagcgtcca tctttctgcc cttaaaaatc cccgaataac
     1501 cgaaaacaag aacaaggata aatatcaatg cctgaaaaaa ggctggagga taacaatagt
     1561 ggaaaatcct gcatctgtga agttatctat aagaagcccc ataaaaaagc tgataaaata
     1621 gatgcttgct gcagcattaa agagggggta tagaagattt gcaggatagc ggaaagtcaa
     1681 aaacaactcg gcgaaaaata gaagaagagc ggtgatagca aaaagcccaa gatttgaatt
     1741 gaagaattct aaagatccat caggatttga gacggttctt gagaaatagc ttgctatccc
     1801 taacagtaca agaataaaga gaaatcctgc aatctttgcc aagaccagag ataatacgct
     1861 tttggacatc aaggataata tggcttttag agtaaataaa ctcttggata aacttaatat
     1921 atcacctagc taatgaaaac cttatggaaa tattcaagat attcaggaaa aaacctgtcg
     1981 aggagtttat ctcatttgag gtcgtagaga aggtgagcgg cgattatacc gagtttgaaa
     2041 gaaagcttac caagcaaagc cagataatcc tcatcaccgg gaggagaggc tcaggaaaga
     2101 cagcgcttgg catgaagata cttgagctat tttgctccaa gtcgcagaga aaatgctatg
     2161 cactgggctt tttggatgca aagcttccct ggaaaataaa aaagatagac gatatagagg
     2221 gtgtgaagct taattcagtt gtccttatcg acgaaggagc gataaccttc tctgcccgag
     2281 attcgatgaa agaaagaaac aagatgcttg gcaagatgat ggcaatagcc aggcacaaga
     2341 acctcagctt gatactaatc gtgcagaact ctgccatgat agaccttaat gtacttagac
     2401 ttgcagattg cctgctcctc aaagagcctt cgctcctgca aaaagatttt gagagagggc
     2461 cgataaaaaa aatatattcc tctgtaatac ctcattttga aaagacagct gacaaaaaaa
     2521 gccatttttt cgtctgggac gatgatttca gaggcatgct gaaatattca ctgcctgctt
     2581 tttggagcga gcagataagc aagagcttta agaatttctg accgtaaact ttataactaa
     2641 acgaatcggc ggtattaata tgaggtgatt ttatggcagc taaaaagaaa gtaacaaaga
     2701 aaaagtctgg agcaaagaag aagaaaagat aattcttctt ttctttttca tcaaaaccca
     2761 cccctcaatg aagggtggac ttttggtttt ccaattcttc gatttgtcag atgcgctcaa
     2821 tctggtattc ttttgattgc tcaagacttg ggttcatacc ccgcagctcg gctagcggtt
     2881 gggattttta ttgcggagct tgctgtggac cctttttaaa acaataaaat ttataactta
     2941 gccagttctt gtcctgcagg aggtgatttg catgacatgc ggatgttgtg gaggaggaaa
     3001 aaagaagaag aaaaagtaat tcttcttcct tgttttatgg caggaaaaat caacgcttca
     3061 catatacttg taaagacaga gtcagaggca aatatagttt tatatgatgt tacccacggc
     3121 aaggcttttg agggcgtcgc cagggagaaa tcgctttgcc cctctggaaa aaagggcgga
     3181 gagcttggat ggtttggcag aaatcagatg gtaagggagt ttgaggttgc tgcttttttg
     3241 ctcaagaaaa gagagatatc caggcctgta aagacacaat ttggctggca tatcatcaag
     3301 gtgaacgacg ttgcataagg ctggcgatag ggtaaaaatt cacttgaagg atgaaattgt
     3361 cgagggcata tttatgccca atgaagagac tgattctatc gtgataaagc ttgataatgg
     3421 atatactatc gggattgaca aaaagaatgt gaaaaagata gaaaagatag gcgaagcaaa
     3481 gaaaaaagaa gaagctgtaa aaaaaatcaa gaccaaccct tctcttccta agattacaat
     3541 tttgcataca gggggaacca tcgcttccaa agtcgattac aaaacaggag cggtctttac
     3601 ctcgtttaag cctgaagacc tgcttggaat gtttcccgag ctatcgctca tcgccaactt
     3661 tgaatcaaag ctccttgcaa atatgtggtc tgatgatctt agattcaagc atttcgagct
     3721 tattgcaaga gcgatagaaa aagaagtaaa agctggagtg gagggcataa tagtcgggat
     3781 ggggacagac aatcttgccg tcgcatctgc tgcgcttgct tttattatcg agagcacacc
     3841 tgtgcctatc ttgtttgtcg gggcgcaaag aagctctgac aggggcagct ctgatgctgc
     3901 gatgaatctc atatgcgctg ccgagtttat cgcaaaaagc gactttgcag gtgtcgggat
     3961 atgcatgcac gagaatatgg atgataaaat cagcgcaata tttccgccct gcaaaacaag
     4021 aaagttacac agctccagaa gagatgcatt caaaactgtg aattctactg tgattgcacg
     4081 gatcaattat gacaccagga atatagatta tactcatgat tataccagaa aggacaagaa
     4141 aagggatttg gcagtcaagc ccaacatgga agaaaaagtg ggattattaa aagtacatgt
     4201 caacatgttc cccgaacagt tttcgttttt tgagggctat aaggggctgg taatcgaagg
     4261 aacaggcctt ggacatatac ctggccaggc tcctgacgat atctcagaga tacataagaa
     4321 gatctaccct gctgtaaaaa aagtgatcga ctctggctgc attgttgtga tgaccacaca
     4381 gtgcatttat ggaagggttc agctgcatgt ttacagcaaa ggcactgatg aagtgaaatt
     4441 gggcataata tctggcgagg atatgcttgc tgagactgcg cttgtcaagc tttcttggct
     4501 tcttggtaat cataaagata agaaagagat agaaaaactg atggtaatca atctaagagg
     4561 agagataaac aagcgcatcg agccttctga atatcttgag taagttttaa atccttcaat
     4621 gattactctc ccataatgat ttctgccaag gattatatca ataaaataaa atggagccct
     4681 ggtgagaaca gcactgaata ttccataggc tattgggata atatcgaaaa gaagatcata
     4741 tttatcgatt ttaacgagat aaaaaccgtt gtcggcaatt ttctatcgct tgaccgtgcg
     4801 aaagagacct atatccctat gcaccggata agagagataa gaaagaaggg aaaggttgtg
     4861 tggaaaagga ccactgcgta aattttttaa gcacagacat gctttctttt tttatgataa
     4921 gaacgaaaat cgtatgcact atcgggcctt gttctgaaac tgttgacatt ctttcaaagc
     4981 ttgccaaggc agggatgaac atcgccaggc ttaatttttc acatggaaat cacgacgagc
     5041 atctggcaag gataaagagg ataaatgaag ccaatgaaag ccttcctatc cctgtcggaa
     5101 taatgcttga cacacaggga cctgagatca ggcttgggac ttttgagaaa aaggagaacc
     5161 ttgtggaagg agaggaagtg gtgataactc caagggacgt tgtctgcaca agcaaagtga
     5221 tatctatctc ttataagaaa atcggcaatc tggtaaaacc aggggattat atctatatcg
     5281 ctgacggcac tatcgagcta aaggttaagg gcaaggttga cagcgatgtt gtgtgcgagg
     5341 ttgtgatagg aggagaggtg aacaccagga agaatgttgc agtgcctgga gcaatagtcg
     5401 atctcccctc aataagcaac aaagacaagg aagacataaa attcgggata agaaacggca
     5461 tcgattttgt tgctcaatcc tttgtcaagt cagaggatga tgtatatgac atgaagaagc
     5521 ttcttgccgg cacagacatc ctcacgattg caaaaataga ggaccctatc ggccttaaga
     5581 atatcgatga gataataggg gctgccgacg ggataatggt cgcccgcgga gaccttggcg
     5641 tacagatacc aatacaagag gtcccgtctg cacagaagat gataatccgg aagtgcaacg
     5701 aagcgggaaa gccagttatc gtcgccacac agatgctgga atcgatgaca aggaacccca
     5761 ggccaacaag ggcagaagtg acagatgtag ccaatgcaat atctgatgga gctgatgcta
     5821 tcatgctaag cggtgaaacg gcaataggca aatatccttt aagggctgtc gagatgatga
     5881 cccgtatagc aaaggaaacc gagggaagaa tagggcccga cttctctgtc tttcatgaca
     5941 gcgataaagt ggaggatgca cttagcaagg ccgtatgcca cacagcttat aacataaaag
     6001 caaatgcgat aatcacatgc actttatcag ggcatactgc aaggatgata tcaaggtata
     6061 aacctcaaac ccctatcgtt gctgtcacgc ctaaccttaa ggagataagg aaattgaacc
     6121 tttggtgggg agtgcatccg ctgcttatag aggctccttc tacaactgat gaccttatcc
     6181 aaaactccgt cgatattgtt atgaaaaaag ggcttgtcaa gaaaggagat atcgtagtcc
     6241 ttactgcagg catacctttt acaacccccg gaaatatgaa cctcatgaag atacatgaga
     6301 tttagggata actgattgtc aaaagtgcat atttacacgg caaattctat atgaacgata
     6361 atatcgatgg ctttttgacc atgagctagt ggtaaatttg caatagtttt ttaaaggaaa
     6421 atggttttta gcgacgctat atgactagag tgagtattat cgattactgc agattagtaa
     6481 ggcaaaacct tccttatgtt aaaggctctc ctcgaggcgt ctatgccttt gggcccaatt
     6541 acgcccagac tcaattagtg attaggtact taggagtgga gttgcgccat actcaatgta
     6601 attttggagg cataacttat ctaccactta gccgctcagc tagcggtttt aataattcag
     6661 aggaatatat ggtcacaatg gggggcgtgc ccttttttgc tttttatcca gggacaaggc
     6721 agaggatgaa tgctctttat ctgagaatga tacatgcacc tgaagagtat caagatagtg
     6781 ggaagatgac tgcctctcgg gggaggattg aaacattaaa agaagctttg cttgataaat
     6841 ataggcaggt gcacctttca tcacagccac aggagaggga tagcatagct ctcgcaagaa
     6901 tacaagatac attatagtaa attaaatttc ttagcagaaa tccccaccac ttgtggttgg
     6961 ggatgaatgc gtaacactta taggatggct aattctgtga ttatctgttg gtaaaatgat
     7021 gagcctcaaa aaagaggtga ggcccaatga cactttacc
//
LOCUS       DASRUK010000067         3708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2064547, whole genome shotgun sequence.
ACCESSION   DASRUK010000067 DASRUK010000000
VERSION     DASRUK010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2064547"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..258
                     /locus_tag="VFF28_03130"
     tRNA            186..258
                     /locus_tag="VFF28_03130"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:219..221,aa:Val,seq:gac)
     gene            304..1380
                     /locus_tag="VFF28_03135"
     CDS             304..1380
                     /locus_tag="VFF28_03135"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZX44654.1"
                     /translation="MPKKKEDYSKFFFIFFTLFMVVITFLVIRPFIAPLLASVFFAYL
                     FYPIYKWLLKWIRNRYACSLIVTLVIIAILIVPTFFIINTLTKEAYVNYLTTKQKILG
                     LGAVFKTCNPQTPFCGFISYMGIILDDPQVKYHLQDSLEGVSSYVVNNVAGLIFSIPR
                     LLLNFFIMTFTLFYLLIDGPSIIQGFRNALPLRDIYKRHLFEKFGMTIHAIAYGYIVV
                     AVIQGALGGIGFYIFGLPSPVIWGIVMMFTALIPFLGTAIVWFPAALFKLISGISSNN
                     TNEIMMASLFMIYGAVVISSIDNLIRPKIIGDKANVHPVMILVGVFGGLYLLGSVGAI
                     VGPLVLSLFLTFVEAYVTERKERA"
     gene            1377..2864
                     /locus_tag="VFF28_03140"
     CDS             1377..2864
                     /locus_tag="VFF28_03140"
                     /EC_number="2.4.2.57"
                     /inference="COORDINATES: protein motif:HMM:TIGR03327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP phosphorylase"
                     /protein_id="HZX44655.1"
                     /translation="MKLKVKDVDIRTGGPLICILSEIDADKMDLHNGDRVKITSKGKI
                     ETVLINIGESKKAVCPGHIGLYEEVLDSLGVVHNDNVEMIPARKPLSLDFIKRKLDGY
                     KLSKEQIGQIVWDVVNNKLTGVELTYFVAACYLNELDVHETVFLTKAMASHGNILRLN
                     RSVIADKHCIGGVAGNRTTMVIVPILAAAGVTIPKTSSRSITSPAGTADTMEVLANVC
                     IPLERMKKVVQKTNGCIVWGGALNLAPADDKIIRVERPLLIDAKSQLLASVMAKKASV
                     SASHLLIDIPTGKGSKLPDVRKARQLKQDFICLAGKLGIKSHVIITDGKEPVGNGIGP
                     ALEARDVLWTLEGDGPLDLRKKCLMMAGILLEMAGKAKKGEGQWLAQEILESGKALEK
                     MKEIIEMQGKIATEPSQIKLGKIRLDVAAKTKGVVSEISNRAISKIARVAGAPECKGA
                     GIYLHKHVGDKVKKGENIYTVFCHDKGKLDFVKYAVENLDGFLIS"
     gene            3101..3664
                     /locus_tag="VFF28_03145"
     CDS             3101..3664
                     /locus_tag="VFF28_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44656.1"
                     /translation="MRGKKQERILRVMLNNKELSKNEISKQALCSRRWVIQFLQKLEK
                     KGLVKNTKILDKKKLISYWVSISKKLKFRSYMIKEPLELLKKANMEYALTTYQAENIV
                     QHFLFPSRIDIYIKEADLDGWHSLMTKNGLYGKGNVRITIEDEHVMYQKRVINGLAIV
                     SLPQLIVDLIKEGGPCVEAAEMLMKNV"
BASE COUNT         1128 a          694 c          905 g          981 t
ORIGIN      
        1 tttggtataa tcagcaaggt aaggggccat tgtaagtttg ttcaattgat ttctcggata
       61 atcttgcata acagggcaag actatcatat tttaacagga ttcagggata atttgtgcgc
      121 aaagcctaat cggagttatc gtatggcaag ttttttaaac cacttgttga ttctatccta
      181 tttacgggcc cgtggtctag tggttatgac gtcacctcga cacggtggag gtcaagagtt
      241 cgaatctctt cgggcccacc ttatcataag ttttataagc aagggctgtg taaataggtg
      301 catatgccca agaaaaaaga ggactattcc aagtttttct tcatattttt cacactattc
      361 atggtcgtga ttacttttct tgtgataagg ccgtttatcg caccgctgct tgccagcgtg
      421 ttttttgcgt acttgttcta ccctatctac aaatggcttc tgaagtggat tcggaacagg
      481 tatgcctgct cacttatagt cactctggta atcatcgcta tcctgatagt gcctactttt
      541 tttatcataa atacactgac caaggaggca tatgtcaatt accttacgac aaagcaaaag
      601 atacttggac ttggcgctgt ctttaagaca tgcaatcctc aaacaccatt ctgcggcttt
      661 atcagctata tgggcatcat acttgacgac ccccaggtaa aatatcacct tcaagattcg
      721 cttgaagggg tttcatccta tgtggtaaat aatgttgctg gccttatctt ttccataccc
      781 cggcttttgt tgaatttctt tatcatgaca ttcactcttt tttatcttct tattgacggg
      841 ccttctataa tccagggatt tagaaacgca cttcccctga gggatatata caagaggcac
      901 ctgtttgaga agtttgggat gacaatccat gccatcgctt atgggtatat agtcgtcgca
      961 gtcatccaag gggctcttgg aggcatagga ttctatatct ttgggcttcc ttcccctgtg
     1021 atatggggga tcgtcatgat gtttactgca ctaatcccat tcctgggaac agccatcgtc
     1081 tggtttcctg ctgcactttt caagctgatc tccggaatct cttcaaacaa tacaaatgag
     1141 ataatgatgg cttctctatt catgatctat ggggctgttg tcatcagcag catcgacaac
     1201 cttataagac ctaagataat cggcgacaag gcaaatgtcc accctgtgat gatcctggtc
     1261 ggggtatttg gcgggcttta tcttttggga agtgtaggtg cgatcgtagg ccctcttgtg
     1321 ctgtcgctat ttttgacatt cgtagaggca tatgtgactg agcggaagga aagggcatga
     1381 agctcaaagt aaaagatgtg gatataagga caggcggccc tctgatatgc atccttagcg
     1441 aaatagatgc tgataagatg gatctgcata atggtgacag ggtaaagata acaagcaagg
     1501 gaaagataga gacagtgctt ataaatatcg gggaaagcaa gaaagcagtc tgtcctggcc
     1561 atatcgggct atatgaggaa gttcttgact cgcttggtgt tgtccataat gacaatgtag
     1621 agatgatacc agcaagaaag ccattgagcc ttgatttcat caaaagaaag cttgacggct
     1681 ataagctttc aaaagagcag atcgggcaga tagtgtggga tgttgtcaat aacaagctga
     1741 caggagtcga actcacttat tttgtcgctg catgctatct taacgagctt gatgtgcatg
     1801 agacagtgtt tcttacaaag gcgatggcat cgcatggaaa tatacttcgg ctgaaccgca
     1861 gcgttattgc tgacaagcac tgcataggag gagtagctgg aaacaggaca accatggtca
     1921 tcgtaccgat acttgcggct gcaggggtta caatccccaa gaccagttca aggtcaataa
     1981 caagccctgc agggacagca gacacaatgg aagttcttgc caatgtatgc atccctcttg
     2041 aaaggatgaa gaaagtggtc cagaaaacaa atgggtgcat cgtatgggga ggagcactta
     2101 accttgcacc tgctgatgac aagataataa gggttgagag gccgcttttg atagatgcaa
     2161 agtctcagct gcttgcttct gtcatggcca agaaagcttc tgtaagcgca agccatcttc
     2221 taatcgatat tcctacaggc aaaggctcaa aattgcctga tgtaaggaaa gcaaggcagc
     2281 taaagcagga ttttatatgc cttgcaggaa agctcgggat aaaatcgcat gttatcatca
     2341 ctgatggaaa ggagccggta ggcaatggca tcggacctgc gcttgaggca agggatgttc
     2401 tttggacgct tgaaggcgac gggcctcttg accttaggaa gaaatgcctg atgatggctg
     2461 gaatactgct tgagatggca ggaaaggcaa aaaaaggaga agggcaatgg cttgctcaag
     2521 agatacttga gagcggcaaa gcgcttgaaa agatgaagga gataattgag atgcagggaa
     2581 agattgcaac agagccttcg caaatcaagc ttggcaagat aaggctggat gtagcggcaa
     2641 agaccaaggg tgtagtttca gagataagca acagggcgat atcaaagata gcaagggttg
     2701 ctggtgctcc tgagtgcaag ggagctggaa tctacctgca taaacatgtg ggagacaaag
     2761 tcaaaaaagg agaaaacata tatacagtgt tctgccatga taaaggcaag cttgatttcg
     2821 tcaagtatgc tgttgaaaat ttggatgggt ttctaatttc ttaaggaagc aagctagggc
     2881 taatagcgga aggggagatg ctcaaaagag gggtaaatta taagacgatt gtcagcctgt
     2941 ttaatcagga aaattctgcc tagatcaacg agaagacaaa gctgggagca gagagatatc
     3001 atatcaaagg catgaggttt atgataattg accggaagga aatactcaat tgcccacatg
     3061 tgggtaaaat ttaaatacta ttcaaaatac ccacatgctt atgcgaggaa aaaaacaaga
     3121 aagaatacta agagtgatgc ttaacaacaa ggagttaagc aagaatgaga tttctaaaca
     3181 ggcactatgc tcaagacgat gggtgataca gttcctgcaa aagcttgaga agaaagggct
     3241 tgtaaaaaat actaaaatct tggataaaaa gaaactgata tcctactggg taagcatctc
     3301 aaaaaagctt aagttcagga gctatatgat caaggaacct ttagagcttc tcaaaaaggc
     3361 aaatatggaa tatgccctga caacatatca ggctgagaac atcgtccagc atttcctttt
     3421 tccttcaagg atagatattt acataaagga agctgatctt gacggttggc atagtcttat
     3481 gacaaagaat gggctttatg gaaaaggcaa cgtcaggata acaatcgagg atgagcatgt
     3541 catgtatcag aaaagggtta taaatggcct tgctatcgtt tctctgcccc aactgatagt
     3601 tgacttgata aaagaaggcg ggccttgcgt cgaggcagct gagatgctga tgaaaaatgt
     3661 ataaagagtt tgagacaaga acatcatata gatacttgaa agaagtaa
//
LOCUS       DASRUK010000068         5604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1402072, whole genome shotgun sequence.
ACCESSION   DASRUK010000068 DASRUK010000000
VERSION     DASRUK010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402072"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VFF28_03150"
     CDS             <1..780
                     /locus_tag="VFF28_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HZX44657.1"
                     /translation="GDGVNDAPALKKADVGVSMGITGTDVAKEVSKATLIDDNFATIV
                     NAIRTGRDIYDKMIKSARYLLSCNSGEITSVFTAIMLGFPLPILPLQLLLMNLLTDTL
                     PGLGLGFESSDDGIMKRLPRDPKEKPISGKMFLSIIFFGLIMAIGTLWVFSKYMGADL
                     VKAQTAAFTTLVTFQMFAVLSSRSLKPSLKKLNPFTNLWLTGAVILSMAVQSAVIYWP
                     PMQKVFGTVGLAAADWAYVIGVASLGFVLMELSKFATYKKW"
     gene            774..1712
                     /locus_tag="VFF28_03155"
     CDS             774..1712
                     /locus_tag="VFF28_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44658.1"
                     /translation="MVDPQLLFHVFISIASLVILAKSADMVVFGISGYAKKLGVSDYL
                     IGFLVVSIGTAIPELVASIAGAMESQGAIVFGTVFGSNLFKVPLMGLIILIAGKIKNN
                     PEIGTSAPIVTLFIVNLPILLIIDGTLSRIDGVLLLMAFFVYIIRLWHGEGKMGRMKQ
                     DIRLKHIWKDGLIFSLALTALLLSAKYLVHSSIQVSNILGISPYIIGLIVIGVGASTP
                     ELSVQIRSIYGRHQDIAFGNVIGSLVANSALVLGITALFTPVLINTSIIIISSVFMVL
                     GSIYSIIVLSKKEINIRQGIILIGIYAVFIAAEWIF"
     gene            1754..3451
                     /locus_tag="VFF28_03160"
     CDS             1754..3451
                     /locus_tag="VFF28_03160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HZX44659.1"
                     /translation="MQQFIDELLSIIEKRKPDKPSLSKIKTKLSKKYRLKKIPTDIEI
                     LLNAPKEKIDSIKRYLLTKPTRTISGVAVCAIMTKPMPCPHVKKGIGPCTMCPGGPDS
                     IFGDVPQSYTGKEPATRRAIRNNFDAYLQVMNRLEQHIVQGHFPDKLELIIMGGTFPS
                     FPKSYQESFIKDALKAMNDFSRYFFKNHIFAFMKFKHFFELPGNIYDEERLKRIHNNL
                     LALKNKDQSTLESEQRKNETSNIRCVSLVIETRPDYATLKEANQMIRLGCTKVELGVQ
                     TIYDKALKRINRGHNVKDSINATRTLKDLGFKINYHIMVGLPGMTKKEDATALSALFN
                     MQEFNPDMLKIYPCMVIKGTKLYQQWKKKRFSPLSTAKAAEIISEFKKTVPEHVRIMR
                     VQRDIPTFVTEAGVDKTNLRQYIEQLMEKKKIKCRCIRCREPRQNKVSNKIVLNEKAY
                     NASSGNEFFFSAEDEKNDLILGFCRMRFPSKSLRKEITDDSALIRELHVYGEAAQIGK
                     KGKVQHRGLGKALMKKAEDKARVYFRRKMVVISGIGAREYFRKLGYHKQGPYMVKML"
     gene            3659..4582
                     /locus_tag="VFF28_03165"
     CDS             3659..4582
                     /locus_tag="VFF28_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004068558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HZX44660.1"
                     /translation="MDNIEFKPAFIERYGKLTDWEIFKDFSLRFLRRSIRINTLKATE
                     EKIKARLKDWTLKQIPWCKEGFWVEHKEGRRDIGNTVEHQLGYYYVQEAASMIPPVVL
                     DPKPGEFILDMCAAPGSKTSQIAQYMQNKGMLIANDYQYIRLQPLGINLQRMGVTNSI
                     ITLMEGRYFKNADLTFDKILVDAPCSGTGTIRKSLKTIGIWNPGAIKRLAGQQRQLLE
                     TAFNILKPGGTMTYSTCSLEPDEDEAVINYLLGKYENAKIEDIDLNINRSKPVQDFEG
                     EQYSQQVKKCLRIWPQDNDTDGFFVAKIVKE"
     gene            complement(4753..5448)
                     /locus_tag="VFF28_03170"
     CDS             complement(4753..5448)
                     /locus_tag="VFF28_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44661.1"
                     /translation="MIRKLFIREKKNEHGFKKRHMWKLHRYVKKMVKRGFNKKRIEDA
                     LVKSGFPDEVIKVEMRELDMHLEKLDGKKAKSIKTCQPQTAIVKVSYAKDKAAKKDKP
                     RNLTIKSKKSAITLKKSKQKVKRRGERMAKGVGQEQARHFLRDVVPEKNFWINSGPIV
                     RNLEGLLGVLENLEDDKFSYHVNKDKNDFAVWIREVVEDNTLAKTAANAKTKNAMSKV
                     LKERIDKLKRAAV"
     gene            complement(5445..>5604)
                     /locus_tag="VFF28_03175"
     CDS             complement(5445..>5604)
                     /locus_tag="VFF28_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014772162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HZX44662.1"
                     /translation="YYMCTKWFNDGDVHKYFNPYDSPYDSFISFMNVLNDLRIRVKEG
                     PIVKGVVE"
BASE COUNT         1719 a         1172 c         1192 g         1521 t
ORIGIN      
        1 ggagacggcg tgaatgatgc gcctgcactt aagaaagcag acgttggagt gtccatggga
       61 ataacaggca cagacgttgc aaaggaagtg tcaaaggcaa cactaattga tgacaatttt
      121 gccacaatag tcaacgccat caggactggc agggacatct atgacaagat gataaaatca
      181 gcaagatatc ttctttcgtg caattcagga gagataacct ctgtgttcac agcaataatg
      241 cttggatttc cgcttcctat ccttcccctt cagcttctat tgatgaatct ccttaccgat
      301 accctgcctg gcctggggct tggctttgaa tcaagcgacg acggcataat gaaaagactg
      361 ccaagggatc caaaagaaaa gcctatctca ggcaagatgt ttctctctat catatttttc
      421 gggctgataa tggcgatagg caccttgtgg gttttttcga agtatatggg cgcagacctt
      481 gtcaaggccc agacggcagc attcacaacc cttgtgacat tccagatgtt tgctgtactc
      541 agcagcaggt cgttaaagcc ttcgctaaag aagctaaatc cgttcacgaa cctttggctt
      601 actggcgcag ttatcctctc gatggctgtt cagtcagcag ttatctactg gcctccgatg
      661 caaaaagtgt ttggaacagt tggccttgct gcagcagact gggcatatgt gataggggtg
      721 gcgagccttg gctttgtgct tatggaacta agcaaatttg caacctataa aaaatggtag
      781 accctcaact tcttttccat gtttttatat ccatcgcctc attggtgata cttgccaagt
      841 ccgccgacat ggttgttttt ggcatatcag gctatgcaaa gaaattaggc gtctctgatt
      901 atcttatagg atttcttgtc gtctctatag ggactgcaat acctgaactg gtcgcatcga
      961 tagcaggcgc gatggaatct caaggggcga ttgtctttgg aacggtcttt ggaagcaacc
     1021 tcttcaaggt cccattgatg ggcctcataa tacttatcgc aggaaagata aaaaacaatc
     1081 cggagatagg gacaagcgct cctatagtta ccttgtttat cgtaaacctg cccatcttgt
     1141 tgataataga tggaaccctt tcaaggatag atggtgttct tctcctaatg gcattctttg
     1201 tctatatcat caggctatgg catggagaag gaaagatggg caggatgaaa caagatataa
     1261 ggttaaagca tatctggaaa gacgggctta tcttttccct cgccttgacc gccctgcttt
     1321 tatctgcaaa atatctggtc cattcttcga tacaggtatc aaacatatta ggaatatccc
     1381 cttacatcat cggacttatc gtgataggcg tgggagcatc aactcctgag ctgtcggttc
     1441 agataaggtc gatatatggc cgtcaccagg atatcgcttt tggcaatgtc ataggatccc
     1501 ttgttgcaaa ctctgctctg gtcctgggta taacagcgct gttcacacct gttttgatca
     1561 atacctcgat aataattatc tcgtctgtat ttatggtgtt gggaagcatc tactccataa
     1621 ttgtcctcag taaaaaagag ataaacatta ggcagggtat tatccttatt gggatctatg
     1681 cagtgtttat tgcggctgaa tggatattct aaggaccaat cacatttaaa tatctatata
     1741 atttctaaag ttcatgcagc aatttatcga cgagttgtta agtataatcg agaagagaaa
     1801 gccagacaag ccatctctct cgaaaataaa gaccaagctc tcaaaaaaat acaggttaaa
     1861 aaaaatcccc acagacatag agatccttct aaatgctcca aaagaaaaaa tcgacagcat
     1921 aaaaagatac ctgctcacaa aaccgacaag gaccatatca ggcgtcgcag tttgtgctat
     1981 aatgacaaag cccatgccat gcccccatgt gaaaaagggg attgggccat gcacgatgtg
     2041 tccaggaggc cctgattcga tatttggcga tgtccctcag agttacaccg gaaaagagcc
     2101 agccacaaga agggctataa gaaacaattt tgatgcttat cttcaagtta tgaaccgcct
     2161 ggagcagcat atagtccagg ggcatttccc tgataagctc gagcttataa taatgggtgg
     2221 cacctttcca agctttccaa aaagctacca agaatctttc ataaaggatg cattgaaggc
     2281 gatgaatgac ttttcaaggt atttttttaa gaaccatatt tttgctttca tgaaattcaa
     2341 acatttcttt gagctgccgg gcaatattta tgacgaagag aggttaaagc gtatacacaa
     2401 taatctactt gctctaaaga acaaagacca atcgacgctt gagagtgagc aaagaaagaa
     2461 cgagacctca aatatacgtt gcgtctcatt ggtcatcgaa acaaggccag actatgcaac
     2521 tctaaaggaa gcaaaccaga tgattaggct aggctgtaca aaagttgagc ttggcgtgca
     2581 gactatatac gacaaagccc taaaaaggat taataggggg cataatgtga aagactcgat
     2641 taatgcgaca agaacattaa aggatttagg ctttaagata aactatcata taatggtagg
     2701 ccttccaggc atgacaaaaa aagaggacgc tactgctctg tcagctttgt ttaacatgca
     2761 ggaattcaat cctgacatgc taaaaattta tccatgcatg gtcatcaagg gaacaaagct
     2821 gtatcagcaa tggaagaaaa agagattctc accattgtcg accgctaaag cagcagagat
     2881 tatctcagaa ttcaaaaaaa cagtccctga gcatgtgagg ataatgcgtg tccaaagaga
     2941 catccctaca tttgtgacag aagcaggtgt tgacaagaca aaccttaggc agtatattga
     3001 gcagctcatg gaaaaaaaga agataaagtg caggtgcata cgctgccgtg agccaaggca
     3061 aaacaaggtg tcaaataaga tagtccttaa tgaaaaagca tataatgctt catcaggcaa
     3121 tgaattcttc ttctcagcag aagatgaaaa aaatgacctt atccttggct tttgcaggat
     3181 gaggtttcca tccaagtcgc taagaaaaga gataactgat gattcggccc tcattagaga
     3241 acttcatgtc tatggcgagg cagcacagat tggcaaaaag ggaaaagtcc agcatagggg
     3301 gcttggaaaa gcccttatga aaaaagccga agacaaagca agagtctact tcagaagaaa
     3361 gatggtcgtt atttctggta ttggcgcaag agagtatttc agaaagcttg gctaccataa
     3421 gcaagggcct tatatggtta aaatgctata aaaccttgca ctttataaag gaaaaattga
     3481 ttgaaccgtg tcaaaggttt tgcttcggat gcggtagata agttaccgtt gtttacgccc
     3541 aacaggttgg tccctctaaa tggggagatg gaaaaaacag tattggaata tggccgaagg
     3601 atgttttcca gaggtagatt taggtaagct taaataaaaa acttattttc taacttttat
     3661 ggacaacata gagttcaagc cagcattcat tgaaagatat ggtaagctta cagactggga
     3721 gatattcaaa gacttctctt taagattctt aaggcgctcg atcaggatca acaccttaaa
     3781 agccacagaa gaaaagataa aagcccgact gaaagactgg accctaaagc agattccctg
     3841 gtgcaaggaa gggttctggg ttgagcacaa ggaaggcagg agggatattg gaaatacagt
     3901 tgagcatcaa ttaggatact attacgttca agaggcagcc tccatgatcc ctccggtagt
     3961 attggatcca aagcctggag aattcatttt ggacatgtgc gcagcgccag gctccaagac
     4021 ctctcagatt gcacagtaca tgcagaataa agggatgctg atagcaaacg attatcaata
     4081 cataaggctg cagcccttag gcatcaacct gcagaggatg ggcgtcacaa actctataat
     4141 caccttgatg gaaggaagat attttaagaa tgcagatctc acctttgaca aaattctggt
     4201 agatgcgcct tgctcaggca cagggactat aagaaaatcg ctcaagacga ttggcatctg
     4261 gaacccagga gcgataaaaa ggcttgcagg ccagcaaagg caactgttag agacagcatt
     4321 caacatacta aaacccggcg gcacaatgac ctactcaaca tgctcgcttg agccggatga
     4381 agacgaagct gtgataaatt atctgctggg caaatatgag aacgccaaaa ttgaagatat
     4441 cgacctaaat atcaaccgct caaagccagt tcaggacttt gaaggggaac agtattcaca
     4501 gcaagtaaaa aaatgcctga ggatctggcc tcaagataat gacaccgatg gattttttgt
     4561 ggcaaaaata gtaaaggaat aaagccacca caaacaaatc cgtccacaaa ttccttagga
     4621 tttgaggcgg attttttgtg actaagataa aaaaagaata agacaagcca caatcaactc
     4681 tgttacaaat tctttagaag tggaagcgcg gcaataacac tgttcacaag caacaaaaat
     4741 aaaaaaaata gattaaacag ccgctctttt tagcttgtca atcctttctt taaggacctt
     4801 agacatcgca ttctttgtct tggcgtttgc agcagtcttt gcaagagtgt tgtcttccac
     4861 gacctcgcgt atccacactg caaaatcgtt cttgtccttg ttgacatggt aggaaaactt
     4921 atcgtcctct agattttcaa ggactccaag tagtccctca aggttcctta cgataggccc
     4981 gctgtttatc caaaagtttt tttcagggac tacatcccta agaaaatgtc tcgcttgttc
     5041 ctggcctact ccttttgcca tcctttcacc tctcctttta accttttgtt ttgatttttt
     5101 tagcgtgatt gcgcttttct ttgatttgat cgtcaaattc ctgggtttat cttttttagc
     5161 cgccttatcc tttgcatagc tgacttttac tattgcagtc tgcggttggc aggtttttat
     5221 agatttcgct tttttgccgt ccaatttttc aagatgcatg tcaagctctc tcatctcaac
     5281 ttttatcacc tcatccggaa atccgctctt tacaagcgca tcctctatcc tctttttgtt
     5341 aaagcccctc ttgaccattt tcttgacgta cctatgcagc ttccacatat gccgtttctt
     5401 aaagccatgc tcatttttct tctctcttat aaacagcttc ctgatcattc taccacccct
     5461 ttgacaatag gaccctcttt tacccttatt ctaagatcgt ttagtacatt catgaatgag
     5521 ataaaggaat cataagggct gtcataaggg ttgaagtatt tatgcacatc tccatcgtta
     5581 aaccattttg tgcacatgta ataa
//
LOCUS       DASRUK010000069         2391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1234392, whole genome shotgun sequence.
ACCESSION   DASRUK010000069 DASRUK010000000
VERSION     DASRUK010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234392"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..453)
                     /locus_tag="VFF28_03180"
     CDS             complement(142..453)
                     /locus_tag="VFF28_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44663.1"
                     /translation="MDGNFYGQLRQMSAEQYVICLQLPIDEERRARIEKEICYNCLYA
                     AEGFAKYGNEALARANLSMFNSYNQKVRITGSHVRFALKEISKQCPNLSKDVEACWKT
                     V"
     gene            complement(535..2367)
                     /locus_tag="VFF28_03185"
     CDS             complement(535..2367)
                     /locus_tag="VFF28_03185"
                     /inference="COORDINATES: protein motif:HMM:NF033551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HZX44664.1"
                     /translation="MNAGERRSSESRVRENFTHGLMRRRWRNQFEETISLLYFLKQAT
                     LNSDKKYNLQDIPPADAKPKVIFKPYCQSQEFLLPKSLNDFISPGHIARLLSDIIDRM
                     DIKFIVDTYKGGGTSAYEPRMLLKSWILAFIYRIYSSRLVAKNLRENLAFIYISGNQT
                     PDFHTLNNFRLRLKDDIKRIFKQIVIYGIEAGIIEGKDVFVDHTKNEANANKHKIVWS
                     RQVNKQLDKIDIELDELFEHIDKINEDEERIFGDKDLPEQERIGFDSGKVQEVISKIN
                     SDMKIGKITKEDGKGQKQKVRRVRELLERKANYLRKQKILDGRNSYSKTDHDAVAMMM
                     KDKLTIRPAYNEGIAVENGFVLDYIISDSCADSVSFVPLMEGVIGNLGKPPENVNADS
                     AYGNEEDYVFLEKKGVGNYLKFNTFQKEKSGVWRNKIFRLNDFRFGAGKNEFACPNGE
                     ILAFEKEKNMVTATGFVQKISVYRARDGACSNCPSKQKCTSAQSSRTLQVNWNSERLK
                     EQARNNLNSPKGKELRRRRGFEVESVFGDQKLNKLKRRYHLRGLSKVNLEAGLYYISH
                     NIRKIYRKINGKLSEGKEQFFGIEAIETGNPTGMIPFPRNNLAF"
BASE COUNT          596 a          566 c          428 g          801 t
ORIGIN      
        1 tcttgatctt cagactttgc gccgataacg atttctgaca tatataagaa ataattccct
       61 atttaaaaag gttactggtg ccagtgccag gatgaataat caataatgat atcccttttc
      121 aacaatcaca aatttgccta cttaaacagt tttccaacag gcttcgacat ctttgctcaa
      181 gtttgggcat tgcttgctta tttcttttaa tgcaaacctt acatggcttc cggttatccg
      241 gaccttttga ttatatgagt taaacatcga aagattagct ctagcaaggg cttcgtttcc
      301 atatttggca aatccttcag cagcgtataa gcaattatag catatctctt tttctattct
      361 agcgcgcctt tcttcatcaa tagggagttg gaggcaaata acatattgct ctgctgacat
      421 ctgcctaagc tggccataaa aattgccatc cattagacga caagcatcat ggcctgcctt
      481 ggataggttg actgcgctct caaaagatta actggaaagt gaaactgggg ttgtctaaaa
      541 agccaaatta tttcttggaa aaggaatcat accagtggga tttcctgtct caatcgcttc
      601 aatgccaaaa aactgctcct tcccttcact taacttacca ttaattttcc tgtaaatctt
      661 tctgatgtta tgagagatat aatatagccc cgcctcaaga ttcaccttgg ataatcctct
      721 tagatggtac cttctcttta gtttattcag tttttggtcc ccaaagacag actctacttc
      781 aaatcctctt cttcgtctca gctcttttcc tttaggcgaa ttcagattgt tccgagcttg
      841 ttccttaagc ctctcagagt tccagttcac ctgcaaagtc ctcgatgact gggctgacgt
      901 acatttttgc tttgaggggc aattgctgca tgctccatct ctagccctat agaccgatat
      961 tttctgtaca aatcctgtgg cagtaaccat attcttctct ttttcaaagg caagaatctc
     1021 accattaggg caggcaaact cattcttgcc agcaccgaac ctaaaatcat taaggcgaaa
     1081 aatcttattg cgccaaaccc cgctcttctc tttttgaaaa gtattaaact taagatagtt
     1141 gcctactcct tttttctcaa gaaaaacata atcctcctca tttccgtagg cagaatcagc
     1201 attcacattt tcaggcggct ttcccagatt gccaataact ccttccataa gcggaacaaa
     1261 gctcactgaa tcagcacagc tatcgctgat aatatagtca agcacaaatc cgttctcaac
     1321 agcaatgcct tcgttgtatg ccggacggat ggtaagtttg tctttcatca tcatagcaac
     1381 agcatcgtga tcggttttcg agtagctgtt acggccgtca aggatctttt gtttcctaag
     1441 gtagttggcc ttcctttcca aaagctccct tacacgccga accttttgct tctgcccttt
     1501 accatcttcc tttgttatct tcccaatctt catatcacta tttatcttgc tgatgacctc
     1561 ctgtactttt ccgctgtcaa accctatcct ctcctgctca ggcaggtctt tgtccccaaa
     1621 tatcctttct tcgtcttcgt taatcttgtc tatatgctca aagagttcat cgagctcaat
     1681 atcgatctta tcgagctgct tgttgacctg cctgctccag actatcttat gcttgtttgc
     1741 atttgcctca ttcttggtat gatccacaaa aacatctttg ccctcgatta ttccagcttc
     1801 tatgccatat attacgattt gtttgaatat cctcttaata tcatccttta gccttagccg
     1861 aaagttgtta agtgtatgga aatctggcgt ttgattcccg ctgatgtaga tgaaggcaag
     1921 gttttcacgg agatttttgg cgaccagccg gctgctgtag atgcgatata tgaaagcaag
     1981 tatccagctt ttcaagagca tccttggctc atatgcactt gtaccgccgc ccttgtatgt
     2041 gtcgacgatg aattttatgt ccattcggtc gattatatca cttagcagcc gtgctatatg
     2101 gcctggggag ataaagtcat ttaggctttt tgggagcaag aattcctggc tttggcagta
     2161 aggcttgaat attactttcg gttttgcgtc ggcaggtgga atatcttgca ggttatattt
     2221 cttatctgaa ttaagtgttg cttgtttcag aaagtagagt aaagagatgg tttcctcaaa
     2281 ttgatttcgc catctcctcc tcatcaaacc gtgcgtgaag ttttccctca cacggctttc
     2341 agatgacctt ctttctcctg cattcaaagt ttagtccagt atttgacgtt t
//
LOCUS       DASRUK010000070         8272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2067003, whole genome shotgun sequence.
ACCESSION   DASRUK010000070 DASRUK010000000
VERSION     DASRUK010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 8272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8272
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2067003"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..807)
                     /locus_tag="VFF28_03190"
     CDS             complement(160..807)
                     /locus_tag="VFF28_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44665.1"
                     /translation="MVTVSHLVGKIVSSRPILNDAIEQGIISYGNLAEQLVPEIEQEL
                     GKKIRHSAVVMALRRYAEGIQKRQIPKFDYNSEIIMKTNLCDISVRKSPSLFSKLKQI
                     YLIVNYEKGDALNVIHGNYEVSIVSNMRYLEQIKKELAGEKILNIEKDLVSLSLNFSG
                     DFLHTPGIISTAARKLNWENVNIFELVSTLTELTFIVSKKDSAKGYAALQRLVVE"
     gene            879..1634
                     /gene="dapB"
                     /locus_tag="VFF28_03195"
     CDS             879..1634
                     /gene="dapB"
                     /locus_tag="VFF28_03195"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018193669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HZX44666.1"
                     /translation="MNISIIGYGGMGREIERIALAKGIGVKSIIDPVAKEATHKEISK
                     ESLKEVDVAIDFTSPSVAVENIRKVCRLKTNMVVGTTGWYTQLPEVKKLVQSSKVGFI
                     YGSNFSVGVHAYFRIIKEAAKLFNNLPDYDIWGHELHHYRKADSPSGTAKSLAEILTR
                     NIERKNKVVYEMLDRKRADSEIHFSSCRGGPVNFKHTIGFDSEADTITIEHSARHRGG
                     YALGAVLAAQWTLDKKGFYIVDDFIKELLGRGG"
     gene            1635..2513
                     /gene="dapA"
                     /locus_tag="VFF28_03200"
     CDS             1635..2513
                     /gene="dapA"
                     /locus_tag="VFF28_03200"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HZX44667.1"
                     /translation="MFRGVYTAIITPFREDGSVDEACLKKLIDFNIKSGVSGIVPCGT
                     TGESPTLSHQEHDRVIELTVQHVNKRVPVIAGTGSNSTTEAIRLTKHAEDAGADACLL
                     VNPYYNKPTQEGLYRHFKAIADSVKFPCIVYNIKGRTGVNVETATLMRIAKDCKNVIS
                     VKEASGDLRQIKEVIAQRPKGFSILSGDDNMTLELIRAGGDGVISVASNLVPDRIVDM
                     VSAALKGDFATAEKLNKALAPLYEAEFIETNPIPIKCGLALKGMCKEVYRLPMCELEP
                     QNREKLKAVLKQMKII"
     gene            2746..2928
                     /locus_tag="VFF28_03205"
     CDS             2746..2928
                     /locus_tag="VFF28_03205"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013303068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HZX44668.1"
                     /translation="MPVIKIDMWKGRTDEQKAEIIRGITAVFEKIGVPKESITIIINE
                     VDKANWGMKGSQASKQ"
     gene            2938..4194
                     /locus_tag="VFF28_03210"
     CDS             2938..4194
                     /locus_tag="VFF28_03210"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HZX44669.1"
                     /translation="MDYKLPFAKKKIMEVLESYKTPFHIYDEKGIIDNAKKLKKAFGV
                     LNGFKEYFAVKALPNPFILKILKEQGFGADCSSLPELILANKAGITGENIMFTSNDTP
                     ADEFIKAKELGAIINLDDITHLPVLEEAAGIPSLVCFRYNPCNSRKGNEIIGKPEEAK
                     YGLTKEQLFEAYKIMQDKGVKRFGLHTMVISNELNEDYFIETANMLFDLAIEINNKLG
                     IKLEFLNLGGGIGTPYKPTDKAVSYEKIAKGIKKAYDEKMTKNNLVPKIFMECGRVVT
                     GPYGYLVTKVRHVMHKYRDYVGVDACMANLMRPAMYGAYHHITVLGKESAPLNMAYDV
                     VGSLCENNDKFAIQRKMPMIEVGDILIIHNTGAHGHAMGFNYNGKLRSAELLLREDGF
                     VVEIRRAETPDDYFSTLDFRKLAKFL"
     gene            4331..5359
                     /locus_tag="VFF28_03215"
     CDS             4331..5359
                     /locus_tag="VFF28_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44670.1"
                     /translation="MGQLEDLITDLRDFFFGVYMPQTGHLRFPIDDATAAQVRLKDIS
                     ASLRDSRFQEIHRRLVGLHPEFQAIIGSNRGLAIAGMTYYHDFAETQAAIWMNYLARH
                     VRNVDDKSLGSLNQFVSHLNHLEIGFSNMLYFASNGKEKELDGTIPIKRFKSLFEVVS
                     DYDRLTVDISRAPETTTLTYGWYLSAVQFVTNAVNNSFPLCKPDIMLAVYYTTCPNGQ
                     VYKVIEAQDNAAGVCENGRPWDDEHMWRIFAGYSPRGGGTGGQISAAVIDFADKTCGI
                     QDIDILTRVEGFPVYYFRRGLGLYSVGRSNINRITFPDGHLCLAMPEKLDHGTYIRMF
                     QQVSHPGD"
     gene            5497..6177
                     /locus_tag="VFF28_03220"
     CDS             5497..6177
                     /locus_tag="VFF28_03220"
                     /inference="COORDINATES: protein motif:HMM:NF016831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZX44671.1"
                     /translation="MKQRKAIIGVRHKGCWGSLCTLEYPNIVMEEKGPIGVEKTKDGV
                     RLSAAWDVKFRDRSEFDNFLKSLDKYKMLKVKKVISMYEDHALLKTEWINKSSSYTTV
                     LKNNCLYTSPVAQKDGFEIYEIITEDPDNVVKLISHLDEIGEVKIFEIGRVSKEPNKL
                     NLTDKQSKALQIAISHNFYEWPRKVSLDEVAAMVGMKRRTFQENLRKAEAKVIPQLVG
                     KYLENSCG"
     gene            6158..7447
                     /locus_tag="VFF28_03225"
     CDS             6158..7447
                     /locus_tag="VFF28_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44672.1"
                     /translation="MKTAAAESLDDVLKRIDPKVVGLPKTQIGIPPVDKFTKRGMEIF
                     LMHYGNNLTHGPKDELAFAVADYCHKEGINVMLELNPVRDKRKSSLGSYIDALHTAAS
                     FYRGMVVVEFTRSDKKDADGRVAPYVSVQRLAGRKRLVSVHLDMDREHYTSQRLEQIF
                     NSCLTGQGAKDDDYLNNFYHSKDAGRLRKFIKGQLKVTLRCKDVTNPDEFIRASEAYH
                     YKGEVFDQMKKMDVIIASGGTFTRIKGMIEKLGPTYIGEDKVGPSYVIVTSPDNSAAL
                     ERYFGSIQPGNRLGDGRFMDKEGKLILNSDKQQVISGYAFEPRYDLRVIIAEKEDFKH
                     ASEIAKQIKCKVAGLDNMHISDTGMVTLCASTDKYAASYNYPFTDEGISLKTFAYEWQ
                     KDGPNLKEFPFIVGPGQKVCFVTTGASDLTYVKQRAA"
     gene            complement(7444..>8272)
                     /locus_tag="VFF28_03230"
     CDS             complement(7444..>8272)
                     /locus_tag="VFF28_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44673.1"
                     /translation="QGKQGMLLNMATAYSERGRVDPDDLAGKRGKARIVSAIVREDEG
                     RLYLRLEETLPLTVEWKGYHPSIRGRSKASFVISKPEVYRELLQELGITYNPSRDNGR
                     NLAELQGKEVMTYKQNGQNVAAVQPFSPNFTKSRDIQHLNKGLYGYSTIDLGDGIIHN
                     DVVTREELMKLCPGDYGAALIKIAGPQYDADRGELNLDLRSRLGDSDYSFMFTNEEAI
                     GHFLKASEVSKAEALTGRKMVGLIAPLDNLEWIIASPRDAILDDVLLTRLEMRKPTC"
BASE COUNT         2595 a         1623 c         1873 g         2181 t
ORIGIN      
        1 tctttcaagc atacaataat ttcattgaac cttacaagga gcacaaaact ccttgtatga
       61 ttgaagggat aacttcaaaa atatgggatt gggaagatat ctttatgaat ttttatcagt
      121 catcttagta gtcatactcc aatttaagcc tagcacgcct tattccacaa ccagtctctg
      181 cagcgccgca taccccttgg cagaatcctt cttagaaaca ataaacgtca gttcagtcag
      241 cgtagatacc agctcaaata tattgacatt ttcccaatta agcttacgag cagctgtgga
      301 gattatcccg ggtgtatgca aaaagtcgcc tgaaaaattc aaagataatg ataccaggtc
      361 cttttcaata tttagtatct tctcgcctgc aagctctttt tttatctgct caagatacct
      421 catgtttgag actatgctta cctcatagtt gccatgtatc acattaagtg cgtccccctt
      481 ctcataattc actatcaagt atatctgctt aagtttggaa aagagtgaag gcgactttcg
      541 taccgaaata tcacaaagat tcgtcttcat tattatctca gagttatagt caaacttagg
      601 tatctgtctt ttctgtatgc cctcagcata cctgcgaaga gccatgacca cagcagaatg
      661 ccttatcttc ttgccaagct cctgctctat ctcagggaca agctgctcag caagatttcc
      721 atagctgatt atgccctgct caatggcatc gttcaagatt ggccttgagc ttacaatttt
      781 tcccacaaga tgtgatacag ttaccatgtt acaaattgtt ataatgataa cagaaagttt
      841 aaatagctta taaattttgc ggtttctaag gtgatcagat gaatatttcc ataataggat
      901 atggcgggat gggaagagag atagagcgca tcgcactagc taaaggcata ggtgtaaaga
      961 gtattataga tccagtagca aaagaagcga cccacaagga gattagtaag gaatccctaa
     1021 aggaagtaga cgtagcaata gactttacca gtccttcagt tgcagtagaa aatatcagaa
     1081 aggtttgccg attaaagaca aatatggtag tgggaacaac cggttggtat acccagctgc
     1141 cagaggtaaa aaagctggtc caatcctcaa aagttggatt catctatgga tcaaattttt
     1201 cagtgggcgt ccatgcgtac ttcaggataa ttaaagaggc agcaaagctc ttcaataact
     1261 tgccagacta tgatatatgg ggccatgagc tgcatcacta taggaaggct gactcgccgt
     1321 caggcacagc aaagtctcta gccgaaatac ttacaagaaa catagagcgg aagaacaagg
     1381 tagtctatga aatgctggat agaaagaggg cagatagtga gatccacttc tcaagctgca
     1441 ggggaggccc tgtaaatttc aagcatacaa taggctttga ctcagaggca gacacgataa
     1501 caatagagca ctcagcaagg cacaggggag gatatgccct tggtgcagtc cttgcagccc
     1561 aatggaccct cgacaagaag gggttttata tagtggacga ttttatcaaa gagcttttag
     1621 gacgaggtgg ttaaatgttt agaggcgttt atacagcgat aattaccccg tttagggaag
     1681 atggcagtgt ggatgaagca tgcttaaaga agttgataga ctttaacata aagagcggag
     1741 tctcaggaat agttccatgt ggaacaactg gtgagagtcc gacattaagc catcaggagc
     1801 acgatagggt catcgaactt actgttcagc atgtaaacaa gagagtgcca gtcattgcag
     1861 gcacaggctc aaactcaacc acagaagcga taaggcttac caagcacgct gaagacgctg
     1921 gcgcagatgc ctgcctcctt gtcaatcctt attataacaa gccgacacag gaaggtctct
     1981 ataggcactt taaagctatc gctgactcag tcaagtttcc atgcatagtc tacaacataa
     2041 agggaagaac aggggttaat gtagagacag ctacattgat gaggatcgca aaggactgta
     2101 aaaatgttat ctcagtaaag gaagcatcag gcgacttgag acagataaag gaagttatag
     2161 cccagaggcc aaaggggttt tctatcttga gcggcgatga caacatgacg cttgaactga
     2221 taagggcagg aggagatggc gtgatatctg tcgcatctaa tctggtacct gataggattg
     2281 ttgatatggt gagtgcagca ttaaagggag actttgcgac agcagaaaag cttaacaaag
     2341 ctcttgctcc actttacgaa gcagaattta tcgagacaaa ccccattccc ataaagtgcg
     2401 ggcttgcact caaaggcatg tgtaaggaag tctataggct tcccatgtgc gagctagagc
     2461 cgcaaaacag ggagaagctt aaggctgtac taaagcagat gaagataatc taaatctgtc
     2521 aagaaccacc cattacaata tgggtgcgtg tctaggttat cttcgaacag caagtggttt
     2581 tgacgctcag aattcaacca ataagggttg tttttgagca tcatagaaat ccagaggatt
     2641 tctaggaact tagaaacagc ggtttctaag tcagcagatg cccctgacca aaagatataa
     2701 aaattggtgg aattgtttaa ataatacctt tagagaggga ttgatatgcc agtgataaaa
     2761 atagacatgt ggaaaggaag gacagacgaa cagaaagctg agataataag aggtataact
     2821 gcagtgtttg agaagatagg tgtgccaaaa gaatcgataa caatcatcat caatgaggtt
     2881 gataaggcta actggggaat gaaaggctcc caagcatcaa agcagtaaga gataaatatg
     2941 gactacaaac taccgtttgc aaagaagaag ataatggaag ttctggagag ttataagact
     3001 cctttccata tctatgatga gaaagggata atagataatg caaagaagct aaagaaggca
     3061 tttggtgtac ttaacggatt caaggagtat tttgcagtca aggctctgcc taatccattt
     3121 atcttaaaaa tcctaaagga acaagggttt ggagcagact gcagcagttt gcctgagctc
     3181 atccttgcaa ataaggcagg aattacagga gaaaacataa tgttcacctc taatgatacc
     3241 cctgcagacg aattcataaa ggcaaaggag cttggagcga taatcaatct cgacgatatc
     3301 acccacctcc ctgttcttga ggaggcggct ggaataccct cactggtttg ctttagatat
     3361 aatccatgta attcaaggaa aggcaacgaa ataatcggaa agccagagga ggcaaagtac
     3421 ggcctcacaa aggagcaatt gtttgaagcc tacaagataa tgcaagataa gggagtaaag
     3481 agatttgggc ttcatactat ggtcatatcc aatgagctca atgaagatta ctttatagaa
     3541 accgctaaca tgctctttga cctggccata gaaataaaca acaagcttgg cataaagctt
     3601 gaattcctaa accttggcgg cgggatagga acgccttaca agccgaccga taaggcagta
     3661 tcctatgaaa agatagccaa aggcataaag aaggcttatg atgaaaagat gactaagaac
     3721 aaccttgtgc caaagatatt catggaatgc ggcagggtgg tcactggtcc atacggttac
     3781 ttggtaacaa aggtaaggca tgttatgcat aaatacagag attatgtggg cgtagacgca
     3841 tgcatggcaa acctgatgag gccagcgatg tatggagcat accatcatat cactgtgctg
     3901 gggaaggaat cagcaccact aaacatggcc tatgatgtag tcggcagcct ttgtgaaaat
     3961 aacgataagt ttgcaataca aagaaagatg ccaatgattg aggtaggtga tattctcatc
     4021 atccacaaca caggagctca tggccatgcg atgggattta actacaatgg taagctaagg
     4081 agcgctgaat tgctgctaag agaagatggt tttgtcgttg agatcagaag ggctgaaacg
     4141 cctgatgatt atttctctac attggacttt aggaagctgg ctaaatttct ataatcatct
     4201 tttttgctgg attactttgg ttgctgacat ttttccagaa ataaatggct gaatcatctt
     4261 aatgataatt taatttagag atatttttta tatgctctcc ataggtcgtt tcaccttgtg
     4321 aacataggta atggggcaat tagaagatct aatcactgac ttgagggatt ttttctttgg
     4381 ggtatatatg ccgcaaacag ggcacctcag gttccctatt gatgatgcaa ccgcagcaca
     4441 agtgcgccta aaggatatct cagcctcttt aagggattca agatttcagg aaattcatcg
     4501 ccgcttggtc ggcctacatc ctgaatttca agctataata ggcagcaatc ggggtcttgc
     4561 aatagcagga atgacttact atcacgattt tgcagaaaca caggcagcca tctggatgaa
     4621 ctatttagcc cgtcacgtgc gaaatgttga cgataaatct ttaggttcac tgaaccaatt
     4681 tgtttctcac ctaaaccatc tcgagattgg atttagcaat atgttatatt ttgccagcaa
     4741 cggaaaggaa aaagaattag atggaacaat ccccatcaaa cgctttaaat cactttttga
     4801 agttgtctcc gactatgatc gtctaactgt cgacatttcc agagccccag aaacaacaac
     4861 cctaacctat ggatggtatc taagtgcagt tcaatttgta acaaatgcag tcaataactc
     4921 ttttcctttg tgcaaaccag atataatgtt ggcagtttac tatacgacct gtccaaacgg
     4981 tcaggtatac aaagttattg aggcccaaga caatgcagct ggagtttgtg aaaacggcag
     5041 gccctgggat gatgagcata tgtggagaat atttgcagga tattcgccaa gaggaggagg
     5101 cactgggggg cagatcagtg cagcggttat tgattttgct gataaaacct gcggcataca
     5161 agatatagac atccttacta gagtggaagg gtttcccgtt tactattttc gtagaggatt
     5221 aggattatat tcagttggca ggtcaaatat caaccggatt actttccccg atgggcacct
     5281 gtgtctggca atgccagaaa aattagatca cggtacatac ataaggatgt ttcaacaggt
     5341 aagccatcct ggggattagc ctattttgtc cttgacttgg tgataagcag atccgccaat
     5401 cggcagcgtg ttgatcgttg gcgcacgata ttgccacccg tcagtagcta aaaattcata
     5461 agatttatat atgacctatg tcactaccat gtaattatga aacaaagaaa agcgattata
     5521 ggcgtaaggc ataaaggctg ctggggcagc ttatgcactc ttgaataccc caacatagtg
     5581 atggaggaaa aagggccgat aggtgtcgag aagacaaaag acggtgtaag gctctcagca
     5641 gcatgggatg tcaagttcag ggacaggtca gagtttgata attttttgaa gtcactggat
     5701 aagtataaga tgctcaaggt aaaaaaggtt atcagtatgt atgaagacca tgctcttctt
     5761 aagacagaat ggataaacaa aagttcaagc tatactactg tgctaaaaaa taactgcctg
     5821 tatacatctc ctgtggcgca aaaggatggc tttgagatat atgagataat caccgaagat
     5881 ccagacaacg tcgtcaagct aatatctcat cttgatgaga taggagaggt taagatcttt
     5941 gagatcggca gggtcagcaa agagccaaac aagcttaacc ttacagacaa gcagtcaaaa
     6001 gcacttcaga tcgctatctc gcacaatttc tatgaatggc caagaaaggt ctcccttgat
     6061 gaggtcgcag cgatggtagg tatgaaaaga aggaccttcc aggagaacct gagaaaagca
     6121 gaggcaaaag ttatacctca gctggtgggt aaataccttg aaaacagctg cggctgagag
     6181 cttggatgat gttttgaaaa gaatagaccc taaagtagtt ggactgccca aaactcaaat
     6241 agggattcct ccagtagaca agttcactaa gaggggcatg gagatctttt tgatgcatta
     6301 tggtaataat cttacccatg gccccaaaga tgagcttgct tttgcagtgg cagattattg
     6361 ccataaagaa ggcatcaatg taatgcttga gctaaatcca gttagggata aaaggaaaag
     6421 ctcgttaggc agctatattg acgcattgca tacagcggcc tctttttatc gcggtatggt
     6481 tgtggttgaa ttcacaaggt ctgataaaaa ggatgctgac ggaagggtag ccccttatgt
     6541 cagcgtgcaa aggcttgccg gcagaaagag attagtctct gtccatctag acatggatag
     6601 ggaacattat acttcacaaa ggctggaaca gatattcaac tcctgtttga caggtcaggg
     6661 agctaaagat gatgactatc tcaataactt ttatcactct aaagatgcag gcaggcttag
     6721 gaagtttatc aaagggcagc tcaaggtaac gttgagatgc aaagatgtta ccaatcctga
     6781 tgagtttata agagcttcag aagcttatca ctacaaagga gaggtatttg atcagatgaa
     6841 aaagatggac gttataattg catcaggagg gacatttaca aggatcaaag ggatgatcga
     6901 aaagctgggt ccaacataca tcggcgaaga caaggttggg ccctcttatg ttattgttac
     6961 ttcaccagac aattctgccg ccttggaaag atactttgga agcatacagc caggtaaccg
     7021 attaggagat gggcgattca tggataaaga gggcaagcta attttgaatt cagacaaaca
     7081 acaggtaatt agtggatatg cttttgagcc tcgttatgac cttagggtta tcattgctga
     7141 aaaagaagat ttcaaacatg caagtgaaat tgccaagcaa ataaagtgca aagttgcagg
     7201 ccttgacaat atgcatataa gtgatacagg gatggtaacc ctttgtgcat caacggataa
     7261 atatgccgca tcttataatt atccctttac agatgagggt atttcattga aaacatttgc
     7321 atatgaatgg caaaaggatg gcccaaatct aaaggaattt ccttttattg tcggaccagg
     7381 acagaaggtc tgttttgtca ccaccggtgc aagcgactta acttatgtca agcaaagggc
     7441 agcttaacaa gtcggcttcc tcatttcaag tcttgtcaac agcacatcat ctagaatagc
     7501 atcccttggt gaggcgataa tccattcaag gttgtctaaa ggagcgataa gtcctaccat
     7561 ctttctgcct gtaagtgctt ctgctttaga aacctcgctt gccttaagaa aatgccctat
     7621 agcctcttca ttggtaaaca taaaactata gtctgaatcc cctagacgac ttctaagatc
     7681 aaggttaagt tcgcccctat ctgcgtcata ctgaggacct gctattttaa ttaaggcagc
     7741 gccataatct ccagggcata atttcatcag ttcctctctt gttaccacat cgttgtgtat
     7801 gattccatct ccaagatcta tcgtggaata cccatacagt cccttattca gatgctgtat
     7861 atccctgctt tttgtaaaat tggggctgaa cggttgcaca gcggctacat tctgtccatt
     7921 ttgtttatag gtcatcactt cctttccctg cagctctgca aggttcctgc cgttgtctct
     7981 ggagggatta taggttatac ctaactcttg aagcagctca cgataaactt ctggcttaga
     8041 gatgacaaaa cttgccttgc ttctgcctct gatgcttgga tgataacctt tccattccac
     8101 tgtcaatggc agggtctctt caagccttag ataaagtctt ccttcgtcct ctctgactat
     8161 tgctgaaact attcttgcct tccctcgctt cccagccaga tcatcaggat ctaccctgcc
     8221 tctctctgaa tatgcggttg ccatattaag tagcataccc tgctttcctt gc
//
LOCUS       DASRUK010000071         4734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_567661, whole genome shotgun sequence.
ACCESSION   DASRUK010000071 DASRUK010000000
VERSION     DASRUK010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567661"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2180)
                     /locus_tag="VFF28_03235"
     CDS             complement(<1..2180)
                     /locus_tag="VFF28_03235"
                     /inference="COORDINATES: protein motif:HMM:NF010337.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZX44674.1"
                     /translation="MIKDFTPRIYQETIFNTCTTKNTLVVLPTGLGKTNIFLMLAAHR
                     LTLYPLSKILFIGPTKPLIDQYLLVFKKHFDIAEEKMAVFTGNVKPEKRAELWKSATI
                     IFSTPQGLENDVISGRINLEDVSLLGVDEAHRAVGDYAYVWLASQYMKKAKYPRIIGL
                     TASPGSELGKIEEVCKNLSIEKIEARTDEDADVAPYIQEIDVTWVKVNLPPIFKEVLR
                     YFELFLKERLEKLKEWGVLQRNDIQLKYISRTELLALQGELRGRAASGERDFVVWNAI
                     SVLAEIMKVQHAVELLETQGVTALHKYLEKLQSEGASGKTKAIKSVVGDINFRSALIK
                     TEKLFSENIQHPKLIELQKIIQKEISEHTDSKVIVFNQYRDNALDIVGVLNHLNGVRA
                     QLFVGQTKKKDTGLSQKEQKEILDKFRASEFNVLVATSVGEEGLDVPQVNLVVFYEPI
                     PSAIRTIQRRGRTGRHESGKVIVLMALETRDVGYKWSAHHKEKRMYRTLDKLKNRVFA
                     KATPGLEKYTAPLAKVKIIADFREKGSPVIKELIELGANMQIDKCESADFLLSSRVGV
                     EFKTAEDFVNSIIDGRLLVQIKNMKNNFERPLVLIEGDQDIYSVRNVHPNAIRGVLST
                     IAISYGIPVLFTRNSKESAGLLFIIAKREQDESGSDFSMHALKKDMSVHEWQEYIISA
                     LPGVGPGLAKPLLREFRSLKNVINADVDSLQKVEKIGPIKAKKI"
     gene            complement(2206..2865)
                     /locus_tag="VFF28_03240"
     CDS             complement(2206..2865)
                     /locus_tag="VFF28_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44675.1"
                     /translation="MILNKLRIEDLVELTGSVVDARSEYQDMQNFWGATLDVNTPHRL
                     FCRMFNPIVFPLGNNALESYRRLIVELAEDPMISGVSVYNNGPWRRLVKNGGYYEIIE
                     RQRLIGEIMKVSDLENDNKVRVMAAIAANDRYHYKYLEAVVGPKDHPNLNTTLSFTEY
                     TKGQKAIVVGYELMFPASKAGLFRAHTSWTNYRLAYCLWVPDGYKDLPTPASQRKKIV
                     I"
     gene            complement(2923..3477)
                     /locus_tag="VFF28_03245"
     CDS             complement(2923..3477)
                     /locus_tag="VFF28_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZX44676.1"
                     /translation="MKEIAPKVWKLNVDSNVYFLDFKEKIVIDTGPRAYASVVKEELS
                     KKISLDKIEKVIFTHLHFDHSGNFNLFSNARFYASAVEIALFNENKMNAILDPLLAKK
                     FDIKLEALENFPGLEVISTPGHTKGSVCIYYPKEKVLFSGDTLFFNGFGRIDFPFSDP
                     GKMEESLEIIGKIGYKILAPGHDY"
     gene            complement(3474..3803)
                     /locus_tag="VFF28_03250"
     CDS             complement(3474..3803)
                     /locus_tag="VFF28_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44677.1"
                     /translation="MKMNAFNWVEIVLLFVLALGFLLSVLAPSAALSYATIFAAGMIS
                     GRVIYSRKKSMVFPYVIIVAGFLLGYLIGARYGDYRIIALLYIIGASFSYYIHEKGLI
                     SGHRNWP"
     gene            complement(3800..4564)
                     /locus_tag="VFF28_03255"
     CDS             complement(3800..4564)
                     /locus_tag="VFF28_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44678.1"
                     /translation="MGYSVDLSAKIKTYYKFPKPELYGFIAVIAILSFIISFSEWGKD
                     EFNFAIGIFNWFNSALIVTFVLLIHNFVQRVAGWNKGYNVEYKAWLTGLFIGVIAVFL
                     TRGRIWVLLPGGIMIHHLTGHRLGHFRYGLNYFQHGMISVAGPISLISLAIVFKALNE
                     ALSNSLISKMVTFCVVLAIYEILPIPPLDGSKLFFGSRMAYVFFLSTIIIAGILILAD
                     ANPLMTIIGSLIVGLILWLLYYIFFERKVWGGPWAK"
BASE COUNT         1290 a         1110 c          869 g         1465 t
ORIGIN      
        1 ttgatctttt tcgcttttat tggccctatc ttctcgacct tttgcaggct gtccacatcg
       61 gcatttatga cattttttag cgacctgaat tcacgaagaa gtggctttgc aagtcctggt
      121 ccaacacctg gcagtgcgct gatgatatat tcctgccatt catggacgct catatccttt
      181 ttcaaagcat gcattgaaaa atcgcttcct gactcatctt gctctctttt tgcgatgata
      241 aacagaagtc ctgcgctttc tttgctgttt ctcgtaaata atactgggat gccgtagctt
      301 atggcgatag tggataatac cccccgtata gcattcggat gcacatttct cacagaatag
      361 atatcctggt ctccctcgat taatacaagc ggcctttcaa aattattttt catatttttt
      421 atctggacaa gcagcctgcc gtcgatgata gagtttacaa aatcttctgc agtcttgaat
      481 tctaccccta ctcgtgaact gaggagaaag tctgcgcttt cgcacttatc tatctgcata
      541 tttgccccaa gctcgataag ctcttttatc acaggagagc ctttttcccg aaaatcagct
      601 ataattttta cttttgccag gggagcagta tatttctcaa ggcccggagt cgctttggca
      661 aatacccggt tctttaattt atcaagcgtc ctatacatcc ttttttcctt gtgatgtgca
      721 gaccacttgt atcctacatc tcttgtttca agagccatta agactattac tttgcctgat
      781 tcatgccttc cagtccttcc tcttctctgt attgtccgta ttgcagaagg tatgggctca
      841 tagaaaacga cgaggtttac ttgcggaaca tcaagccctt cttcacccac agacgtagcc
      901 acaagaacat tgaactcaga agccctgaat ttgtcaagga tttctttttg ctctttctgg
      961 ctaagtcctg tatctttctt cttggtctgc ccgacaaaaa gttgcgccct caccccatta
     1021 agatggttta gcaccccgac aatatccagc gcattatccc tgtattggtt gaatacgatg
     1081 acctttgaat cagtatgctc tgagatttcc ttttggatta tcttttgcag ctcgataagt
     1141 tttggatgct ggatattctc agaaaacagc ttctcggttt tgataagtgc gcttctaaag
     1201 ttgatatcac ccacaacgct cttgatagcc tttgtcttgc cgcttgctcc ctcgctctgc
     1261 aatttttcaa ggtacttgtg cagggcggtg actccttggg tttcaagcag ttcgactgca
     1321 tgctgcactt tcattatttc tgcaagaaca gagatagcgt tccatacgac aaaatcacgc
     1381 tcgcctgagg cagctcttcc tctaagctca ccctgtaggg caagcagctc agtcctgctt
     1441 atatatttca actggatatc atttctttga aggacccccc attccttgag cttctcaagt
     1501 ctttccttca aaaatagctc aaaatacctg agcacttcct tgaatattgg cggcagatta
     1561 acttttaccc aggtgacatc tatctcctgg atgtatggcg caacatcagc atcctcatca
     1621 gtccttgctt caatcttctc tatgctcaga tttttgcaga cctcttctat cttcccaagc
     1681 tcagagccgg gtgaagcagt aagcccgatt attcttggat actttgcctt tttcatatat
     1741 tggctggcaa gccagacata agcatagtcc ccgacagctc tgtgtgcctc atcaacccca
     1801 agcaaggaaa catcttcaag attgatcctt ccagaaataa catcattctc aaggccttga
     1861 ggagttgaaa agatgattgt tgcgctcttc caaagctcag ccctcttttc tggctttaca
     1921 ttgcctgtga atactgccat cttttcttca gcaatatcga aatgcttttt gaagactagc
     1981 aaatactggt cgataagtgg ttttgtaggc ccgatgaaaa ggatcttgct gagtggataa
     2041 agtgtcagcc tatgggccgc aagcataaga aagatattgg tcttcccaag gcctgtcgga
     2101 aggacgacaa gcgtattttt tgtagtgcat gtattaaata tagtctcctg atagattctt
     2161 ggagtaaagt cttttatcat taagagaaaa gtaaagaatc tttgtttaaa taactatctt
     2221 cttcctttgg gaggcagggg ttggcaaatc cttatagcca tctggtaccc ataggcagta
     2281 tgctagtctg tagtttgtcc aggaagtatg ggcacgaaat aagcctgctt ttgaagctgg
     2341 gaacatcaac tcatacccta caacaatcgc tttttggcct ttagtatatt cggtaaaact
     2401 gagtgtggtg ttgagattgg gatggtcttt aggtcctacc acagcttcta gatacttata
     2461 gtgatatcta tcgtttgcag ctatagctgc catcactcta accttattgt cgttttctaa
     2521 atcagaaacc ttcataatct ctccgataag gcgttgtctt tcaataattt cataataccc
     2581 cccattttta actagccttc tccatggccc attattataa acacttacgc cggatatcat
     2641 cggatcctcg gccagttcca ctatcaatcg tctataagac tctaaggcat tatttccaag
     2701 aggaaatact atcgggttaa acatcctgca gaataacctg tgaggggtat taacgtccaa
     2761 ggttgccccc caaaaatttt gcatatcttg atattcgctc cttgcatcca caacagagcc
     2821 tgtcaattcc acaaggtcct ctatccgtaa tttattaagt atcaactaaa ctgcctcaga
     2881 accccttttt atgtcagaat cactgtcgag ttttaagcct ttctagtaat catggcccgg
     2941 tgctagtatc ttatatccta tctttcctat tatctcaagg ctctcttcca tcttgccagg
     3001 gtcagaaaaa ggaaaatcga tccttccaaa accgttgaaa aacaaagtat cccctgaaaa
     3061 cagcactttc tctttaggat aatagatgca cacagagcct tttgtatgtc cgggggtgga
     3121 aatgacttca agccctggga aattctcaag cgcttcaagc tttatgtcaa atttcttggc
     3181 aagcaaggga tcaagtatcg cattcatctt attttcatta aaaagtgcaa tctcgacagc
     3241 cgacgcataa aatcttgcgt tagaaaaaag gttaaaatta ccgctatggt caaaatggag
     3301 atgggtaaag atgactttct ctatcttatc caggcttatt ttcttgctta attcctcttt
     3361 gacaacactt gcataagctc taggtcctgt atctatcaca atcttttcct taaaatccag
     3421 aaaatagaca ttgctgtcta cattcagctt ccatacctta ggtgctatct ctttcatggc
     3481 cagttgcggt gtcctgagat taaccccttt tcatggatat aatagctaaa tgaggcgccg
     3541 atgatatata gaagcgcaat tatcctatag tctccatacc tggcaccgat gagatagcca
     3601 agtaaaaaac ctgcgactat tatcacataa ggaaacacca tgctcttttt tcttgaatag
     3661 attactctgc cagatatcat tcctgcggca aatattgtgg catagctaag ggcagcagaa
     3721 ggtgcaagca ccgataaaag aaatcctagt gcgagcacga aaagaagtac tatctccacc
     3781 cagttgaaag cgttcatttt cattttgccc aaggacctcc ccagactttt ctctcaaaga
     3841 atatataata gagcagccat aagatcaatc ccacgattaa tgagcctatg atggtcataa
     3901 gcggattagc atcagctaag atgagtatgc ctgcaattat tatcgttgaa agaaagaaca
     3961 cataagccat cctgcttcca aagaaaagct tgcttccatc aagcggaggt atcggaagga
     4021 tttcatatat tgccagtact acacagaaag ttaccatctt gctgataaga gaatttgaca
     4081 atgcctcgtt taaggcctta aacacaatcg caagagatat gaggctgata ggccctgcga
     4141 ctgatatcat cccgtgctgg aaatagttca gcccatacct aaagtggcca agtctatgcc
     4201 ctgtaagatg atgtatcatt atccctccag gtaggagtac ccatatcctt cctcttgtaa
     4261 gaaacacagc tatcactccg ataaaaagcc ctgtaagcca cgctttatac tcgacattat
     4321 atcccttgtt ccatcccgct accctttgga cgaaattatg tatgagaaga acaaacgtga
     4381 ctattagcgc gctgttgaac caattgaata tcccaattgc aaagttaaac tcatctttgc
     4441 cccattcaga aaaagagatg atgaatgata agattgcgat gacagcgata aacccataaa
     4501 gctctggttt tggaaacttg tagtaggttt ttatctttgc cgacaagtca accgagtagc
     4561 ccatttcttt tacaacctag acctaaatat ttaaactttt gcaaaactag taagtattca
     4621 gtcagctagc aaatgaaatc agattttcat agacattttt cccttgctga ttccatgttt
     4681 ccattgccga catcacattt ccaataaacc ttctgccatt ttcatttctt atgc
//
LOCUS       DASRUK010000072         7847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1237326, whole genome shotgun sequence.
ACCESSION   DASRUK010000072 DASRUK010000000
VERSION     DASRUK010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 7847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7847
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237326"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..732
                     /locus_tag="VFF28_03260"
     CDS             61..732
                     /locus_tag="VFF28_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZX44679.1"
                     /translation="MTEYLLIAIDSSSSMLARDFSPDRLESAKSAALSFINMVPKNTK
                     IGIVSFAEDVKSGLHPTKDRRQLSQAIGAIKVSMTGGTNIGDALIKSANMLEQGGNKS
                     VILLTDGQSNFGTDVPYAIEYLKSKGVAVYAIGIATKEGGAVGNKFVSKLGTESISQI
                     AEQTGGKYFFAEAGSSLDENFRLIARLKKSDVSFDLSWIFLVFGLIFLILEWFLASTK
                     YKVIP"
     gene            complement(729..1490)
                     /locus_tag="VFF28_03265"
     CDS             complement(729..1490)
                     /locus_tag="VFF28_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZX44680.1"
                     /translation="MQPEIGFSTLSKSTALKDIMFAAKHKFAWLEIDLDTNTNLKAET
                     VRKIRQTAKDKGIRLIVHTPYFLPTSSLLTEVREAVFRYIEKSCILANKLGADRLTIH
                     PGYREISSITFQSLASNLKVIASIGKKYEVSICLENFPKSETLLCYAAQDMIKMLKSV
                     KGLNATLDVGHANTTNIKAHDYLRGIKDFIMDMHLHDNYGKHDEHNPLGKGNVDFQKL
                     FSMCKKVGYKGPFTLEVLSYKAVLAGRKRILELLA"
     gene            1625..2188
                     /locus_tag="VFF28_03270"
     CDS             1625..2188
                     /locus_tag="VFF28_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HZX44681.1"
                     /translation="MKSMLEIRIHGRGGQGAKTASQFIAESALGEDKFIQAFPEYGPE
                     RAGAPMKAYARISDNPITVYSPVVSPDIVMVIDPTLLKLPDITDGLNESSILIINTPR
                     SAEEIKKKIAFKGKIYTVDATKISIDTVGKNLPNTPMLGALIKLSPFIKIDAIKNCIK
                     SKFQKKLGEEKTNATIRGVDLAYAQVK"
     gene            2221..2505
                     /locus_tag="VFF28_03275"
     CDS             2221..2505
                     /locus_tag="VFF28_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZX44682.1"
                     /translation="MKTNKQLPIGGLIDEAGSSRNFKTGDWKSFRPIVEETKCSSCMF
                     CVAFCPENCIHVKDGKRSDIDLEYCKGCGICANVCPTKAIKMQEEAKFLD"
     gene            2568..3761
                     /gene="porA"
                     /locus_tag="VFF28_03280"
     CDS             2568..3761
                     /gene="porA"
                     /locus_tag="VFF28_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HZX44683.1"
                     /translation="MKAQQTKNLMISKIKPQAMTGTDAAAEAMRQINPDVVPAYPITP
                     QTAIMQDYAGFVANGLVDSEMVRVESEHSAMSCAIGASAAGARVMTATSSAGLALMWE
                     MLGIASGLRLPIVMHLVNRALSAPINIHCDHSDGMGCRDAGWVQIYSENAQEAYDHTF
                     IAMRLAEKTFQPAMVCQDGFITSHGVENVEIQDDAKMKSFVGKHDYPFGLLDIKKPVT
                     HGPLQLPDYYFETRSQQITAMEESKKEFLDIGEELSKITGRKYPFFDGYNLKDAEAAI
                     VVMSSTAGTTKAAIDALRAKGKKVGLLKLRLFRPFPYKEVGEALKNIPNIAVLDRAVS
                     FGANAPLYSEIKNALFDVKYNGSLKSCVFGLGGRNIFIEDIEKTFDGLLDDSLKDYSY
                     IGLRE"
     gene            3758..4687
                     /locus_tag="VFF28_03285"
     CDS             3758..4687
                     /locus_tag="VFF28_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZX44684.1"
                     /translation="MKMNYKQLVEKLQNVKFTPGHRSCAGCGFPMIVRSVLASTDKPL
                     VAASATGCLEVTSTIYPFSSWNIPFVHSAFENAGATISGVESAYRALKKQGKIKEDIK
                     FVAFGGDGGTYDIGLQSLSGALERGHDFLYVCYDNEAYMNTGIQRSSATPYGASTTTA
                     PPGKKSLGKAEFRKDFAKICVAHNIPYVAQASVSNLMDLTAKAEKAFSITGPKVLVVL
                     QPCTLGWQYPAEKTIEIAKLAVDTKFWPLYEVENGKYKLNYKPSKEVPIIEFLKTQGR
                     FKHLLAPESKPVVDKMQENINSEWEKLVKLSSE"
     gene            4966..5292
                     /locus_tag="VFF28_03290"
     CDS             4966..5292
                     /locus_tag="VFF28_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZX44685.1"
                     /translation="MKALYRLHAETCKVFSNSTRLEILNLLRDKEMSVTGLIEKTALS
                     QANISQHLSIMKSKGIVLSDRRGKNIYYRLTNPKIINAFDIIKEILSERLKMEHKLTI
                     NLRSKR"
     gene            5289..5855
                     /locus_tag="VFF28_03295"
     CDS             5289..5855
                     /locus_tag="VFF28_03295"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZX44686.1"
                     /translation="MKIRESGMPQESAWNGFFNPSEILKTIGVTSQIKDIAEFGCGYG
                     TFTIPTARLVNGRVYAIDIEPEMIDIVKKKAEENRLANITIMRRDFLTQGSGLKDASV
                     DYAMLFNILHLEEPINLLKEAYRILRPGGKVGIIHWNYDPSTPRGPPMEIRPKPEQCI
                     EWAVGLGFSNPVRYDLKPYHYGIVGVKE"
     gene            5852..6358
                     /locus_tag="VFF28_03300"
     CDS             5852..6358
                     /locus_tag="VFF28_03300"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HZX44687.1"
                     /translation="MKALLIMIVLILGQVNAVSVEELNDAKASIDSRIACDKLTDEQL
                     EKIGEYYMEQMMPGRAHEIADEMMGLVEGSAAEEQFHINLARRSYCGQNIGMMGWGMM
                     GNYPTYAYYGLGSIFSLILIGAIGLIIWLIYRLTRREASIDILKRRYAKGEISKKEFE
                     DMKKEIRG"
     gene            6363..6665
                     /locus_tag="VFF28_03305"
     CDS             6363..6665
                     /locus_tag="VFF28_03305"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HZX44688.1"
                     /translation="MEKDKITLIWIAIAVIIGLFFLGGLGMGGMMGFGGMVGFGMGFG
                     FIFMLLFLGLIIWLIVTLANTPPLNEQDSLAILKRRYASGEITKKEYEEMKKEIGR"
     gene            6672..6995
                     /locus_tag="VFF28_03310"
     CDS             6672..6995
                     /locus_tag="VFF28_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HZX44689.1"
                     /translation="MKIGIIMGTNEPETVWNAFRFGNSALKENHIVKIFLLNKGVEIE
                     DIKSEKYNISEQTDSFLKNKGQILACGTCLKSREKEGSNVCPISTMKDLLKVVEESDR
                     VLTFG"
     gene            7168..7533
                     /locus_tag="VFF28_03315"
     CDS             7168..7533
                     /locus_tag="VFF28_03315"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZX44690.1"
                     /translation="MISAQGAAFGTISLNLSIGAFFIFFLIVTSAVGLLLGLFVQHRT
                     WCRFCPVGSMGNLIGRGKYQLKIDSKKCTDCGICSKVCPMQLNPNSHKKGSVDIVREP
                     DCIKCRLCVNSCPSKALEF"
BASE COUNT         2484 a         1465 c         1808 g         2090 t
ORIGIN      
        1 actataacta aataaagatt tcggtgatgg aacatatagt cctaagaagt tagtttatat
       61 atgactgaat acttacttat tgcaatagat tcctcgtcct ctatgcttgc ccgcgatttt
      121 agccctgacc ggcttgagtc tgcaaaaagt gcagcattat ccttcatcaa catggtgcca
      181 aaaaacacaa agataggcat agtttccttt gccgaggatg taaagtcagg cctacaccct
      241 acaaaagacc gtaggcagct tagccaggct ataggggcaa ttaaggtctc gatgacagga
      301 gggacaaaca tcggcgatgc cctgataaaa tctgccaata tgctggagca aggaggcaac
      361 aagtcagtta tattgcttac agatggccag tctaattttg gcacagatgt tccatatgcc
      421 attgaatatc tgaagagcaa aggtgttgct gtttatgcga taggcatagc gacaaaagag
      481 ggcggagcag ttggcaacaa gtttgtctca aagcttggca ccgagagtat tagccagatt
      541 gcagaacaga caggcggcaa atattttttt gcagaagcgg gttcttcatt agatgaaaat
      601 tttagattga ttgcgagatt gaagaaaagt gatgtaagct ttgacttgtc ttggatattc
      661 cttgtttttg gccttatctt ccttatcctc gaatggttcc ttgccagcac aaaatacaag
      721 gttatacctt aagccaaaag ctctaggatc ctcttcctgc cagcaaggac tgctttatat
      781 gaaagaactt ccagggtaaa tggcccttta taccctactt tcttgcacat agaaaacagc
      841 ttttgaaaat ctacattgcc cttcccaaga ggattatgct catcatgctt tccatagttg
      901 tcgtgcagat gcatgtccat gataaaatct ttgatacctc taagataatc atgcgccttt
      961 atattggtgg tattggcatg gccgacatca agtgtggcat ttaatccctt taccgattta
     1021 agcatcttta tcatatcttg cgccgcgtag caaaggagag tttccgattt ggggaaattc
     1081 tccaaacata tactgacttc atacttcttg cctatgcttg cgatcacctt aaggttgctt
     1141 gcaagcgatt ggaaggtgat agaagatatc tcccgatagc ctggatggat ggtaagcctg
     1201 tctgcgccaa gtttatttgc aaggatgcat gacttttcaa tatatctaaa gacagcttcc
     1261 ctgacttcgg taagcaagct gcttgtagga agaaaataag gtgtatggac tataagcctt
     1321 atacccttgt ctttggctgt ctgtctaatc ttccggacag tctctgcctt taggttcgta
     1381 ttggtgtcca ggtctatctc cagccatgca aacttatgct ttgctgcaaa cattatatcc
     1441 tttaaggcag tgcttttgga tagtgtcgaa aatcctattt caggttgcat tattatatga
     1501 taggttcttt cttatttaaa gcttaacaaa taatgcgtaa aaacaaccat ttgtttgcag
     1561 gcatccgcca aatcctggca aaaaccccaa gttttttata ttagctgtct gctcttattc
     1621 tcacatgaaa agtatgctgg agataaggat acatggcagg ggcggtcaag gtgcaaagac
     1681 agcttctcag ttcatagctg agtcagctct tggcgaggat aaatttatcc aggctttccc
     1741 ggaatatggc ccagaaaggg caggagcgcc gatgaaagca tatgcacgga tctcagacaa
     1801 tcctatcact gtatattcac ctgtcgtaag tcccgatatc gtcatggtca tagaccctac
     1861 actcctcaag ctgcctgaca taacagacgg cctaaacgaa agctctatac tgattatcaa
     1921 taccccaagg tcagcagaag agataaagaa aaagatagct ttcaaaggaa agatatacac
     1981 agtcgatgca accaagattt cgatcgacac agtaggaaaa aaccttccaa atacgcctat
     2041 gcttggagct ttgattaagc tttcaccttt cataaagata gatgccatca aaaattgtat
     2101 taaaagtaaa ttccaaaaaa aacttggcga agaaaagaca aacgcgacca taaggggcgt
     2161 tgatcttgct tatgcccagg taaaataaat aagaccaaca ggcagccact aacaatcaac
     2221 atgaaaacaa acaaacagct tccgattgga ggcttgattg atgaagcagg ctcttcaaga
     2281 aacttcaaga caggagactg gaagagtttc aggccgatag tcgaagaaac aaaatgctca
     2341 agctgcatgt tttgcgtcgc attttgccct gagaactgta tccatgtaaa agatggtaag
     2401 cgcagtgata ttgacctgga atattgtaaa ggctgcggta tctgcgcaaa tgtttgcccg
     2461 acaaaagcaa taaagatgca agaggaagca aaattcctgg attgatcaaa ctgagatgct
     2521 cagtttgatg cttaaaaaat acccctagag gaggattttt tattaagatg aaagctcaac
     2581 agacgaaaaa cttgatgata tcaaagatta agcctcaggc tatgacaggg acagatgcag
     2641 cagccgaggc tatgcggcag attaatcctg atgttgttcc agcttatcct atcactcctc
     2701 agactgcgat aatgcaggat tatgcaggtt ttgttgcaaa cggccttgtg gacagtgaga
     2761 tggtcagggt agaatcagag cattcagcta tgtcatgtgc gataggtgca tctgcagcag
     2821 gagcacgtgt tatgactgca acatcaagcg caggtcttgc gcttatgtgg gagatgctgg
     2881 gtattgcatc aggtctgcgc cttccgatcg tcatgcactt ggtaaacagg gcactatcag
     2941 ctccgattaa tattcactgc gatcattcag atggtatggg ctgcagggat gcaggatggg
     3001 tccagatata cagcgaaaac gcccaggaag catatgatca tacctttatc gcaatgaggc
     3061 tcgcagaaaa aacattccag ccagctatgg tctgccagga tggctttatc acatcccatg
     3121 gcgtagagaa cgtcgagata caggacgatg ccaagatgaa gtcatttgtt ggaaaacatg
     3181 attatccatt cgggctgctt gacataaaaa agcccgtgac tcatggccca ttgcaattgc
     3241 ctgattatta ttttgagaca agaagccagc agataacagc gatggaagag tcaaagaaag
     3301 aattccttga tataggagag gagctaagca agattacagg tagaaaatat cctttttttg
     3361 atggatacaa tttaaaggat gcagaagcag caattgttgt catgagttct actgcaggaa
     3421 cgacaaaagc agctatcgat gctctaaggg caaaaggaaa aaaagtgggc ttgctaaagt
     3481 taaggctttt caggcctttc ccctataaag aggttggaga agccctaaaa aacatcccca
     3541 acattgctgt ccttgacagg gcagtaagct ttggagcaaa cgcccctcta tacagcgaga
     3601 taaaaaatgc actgtttgat gtcaagtata atggcagcct aaagagctgt gtttttggcc
     3661 ttgggggaag aaacatattc atagaagaca tcgaaaaaac ctttgatggt ttgctcgatg
     3721 acagtctaaa agactattcg tatatcggat taagggaata aaaatgaatt ataaacagct
     3781 tgtcgaaaaa ttgcagaatg tcaaattcac accaggccat agaagttgtg ctggttgtgg
     3841 atttccgatg atagtaagat cagtccttgc atcgactgac aagccgcttg ttgcagcatc
     3901 agcaacaggc tgccttgaag ttacctcgac tatttatcca ttttcttcat ggaatatccc
     3961 ttttgtgcac tcggcatttg aaaatgcagg tgcgacgatt tcaggggtcg agtctgccta
     4021 cagggcatta aaaaagcagg gcaagataaa agaagacata aagtttgtgg cttttggtgg
     4081 agatggcgga acctatgata ttggccttca aagcctttca ggagcgctcg aaagaggcca
     4141 tgacttcctt tatgtttgct atgataatga agcatatatg aacacaggga tccaaagaag
     4201 cagtgcaact ccatatggcg catctaccac aacagcgccg ccgggaaaaa aatcattagg
     4261 aaaggcggaa ttcagaaaag actttgcaaa gatatgcgta gcccataaca ttccttatgt
     4321 agctcaggca agcgtttcaa acttaatgga tcttactgca aaagcagaaa aagccttcag
     4381 cattacaggc ccaaaagtcc ttgtcgtcct tcagccttgc actcttggct ggcagtatcc
     4441 agcagaaaag acgatagaga tagcaaagct tgctgtcgac acaaagttct ggccgcttta
     4501 tgaagtggag aacggcaagt acaagttgaa ctacaagccg tcaaaagaag tacctatcat
     4561 tgaattcttg aaaacccaag gaaggttcaa gcatcttctt gcgccagaaa gcaagccggt
     4621 tgttgataag atgcaggaga acataaattc tgaatgggaa aagctggtta agttgagcag
     4681 tgagtaagtg cctttaagcg caagtgcaaa tttagtatag ccatctactt ttaacctgaa
     4741 gataagaagg attatcgtac cttgtaccga acaatgcagc ccaggctaac tcggagaagg
     4801 taagcccaaa tattatcttc tctttgtgct gcaattgtcg tggaatttcg taaaggccgc
     4861 atttataaaa tggctgagaa aggactagaa ggcatctgtt aatagggggg tggtgagata
     4921 acccccgaaa attatttaaa cattagaata ttttaatatg taattatgaa ggcactgtac
     4981 aggttacatg ctgagacgtg caaggtattt tctaactcta caaggttgga aattctgaat
     5041 ttgttgaggg ataaagaaat gtctgtaaca ggactaatag aaaaaacagc ccttagccag
     5101 gcaaatatct cgcagcatct gtcgataatg aaatcaaaag gcattgtctt gtcagataga
     5161 agaggcaaga acatatacta caggcttaca aaccctaaaa tcatcaatgc ttttgatata
     5221 atcaaggaaa tattgtccga aagactgaag atggaacata aacttacgat taaccttagg
     5281 agcaaaagat gaagattaga gaaagcggga tgccgcagga aagtgcatgg aatggatttt
     5341 tcaatcccag tgagatactc aaaactatag gtgttaccag tcagataaag gacatagcag
     5401 aatttggatg cggatatggg acatttacaa tcccaacagc aagattagta aatgggagag
     5461 tatatgctat agatatagag cctgagatga tcgatatagt aaagaagaaa gcagaagaaa
     5521 acaggcttgc taacattaca ataatgcgcc gtgacttttt gacgcaaggt tctgggctta
     5581 aagatgcaag cgttgattat gccatgctct tcaatatttt gcatttagaa gagccgataa
     5641 atctgctgaa agaggcatat aggatattaa ggcctggtgg aaaagttgga ataatccatt
     5701 ggaactatga cccatcaaca ccaagaggcc cgccaatgga gatcaggcca aaaccagaac
     5761 aatgcatcga atgggctgtt ggtttgggat tcagtaatcc cgttagatat gatctcaagc
     5821 cataccatta tggaattgtg ggggtgaaag aatgaaggca ttattaatca tgattgtttt
     5881 gattcttggt caagttaatg cagtcagcgt ggaagagcta aatgatgcta aggcatcgat
     5941 agactccagg atagcctgtg ataaattaac tgacgaacag ctagaaaaga ttggagaata
     6001 ttatatggag caaatgatgc caggaagagc ccatgaaata gcggatgaga tgatggggtt
     6061 ggtagaaggt tcggcagctg aagagcagtt tcacataaac ctggcaagga ggtcttattg
     6121 cggacaaaac ataggcatga tgggttgggg gatgatggga aattatccaa cctacgctta
     6181 ttatggcctc gggagcatat tctctctgat tttaattgga gccattggat tgataatctg
     6241 gctgatttat cggctcacaa gaagagaggc ttcaatcgat atcttaaaga gaagatatgc
     6301 caaaggagag ataagcaaaa aagagtttga ggatatgaaa aaagaaataa ggggatgata
     6361 ttatggaaaa agacaaaatt accctaatat ggattgcgat tgcagtaatt attggcctgt
     6421 ttttccttgg tggtttggga atgggcggca tgatgggttt tggtggcatg gtggggtttg
     6481 gcatgggttt tggattcatt ttcatgctgc tattcctcgg gcttatcatt tggctcatcg
     6541 ttaccttggc aaatactccc ccactaaatg agcaagactc cctggcaata ctgaaaagaa
     6601 ggtatgcttc tggagaaatc acaaaaaagg aatacgaaga gatgaagaaa gagataggca
     6661 ggtgattgag cttgaaaatt ggaattataa tgggaacaaa tgagccagag actgtctgga
     6721 atgcgtttag gtttggcaat agcgcattaa aagaaaacca tatcgtaaag atctttctct
     6781 taaacaaggg tgttgaaata gaggatatta aaagcgaaaa atataatatt tccgaacaga
     6841 ccgattcatt cctcaagaac aaagggcaga tattggcttg cggaacatgc cttaagtcca
     6901 gggagaaaga aggaagcaat gtctgtccaa tctcaacaat gaaagatctg ctaaaagtag
     6961 tggaagaatc agacagggtt ttaacctttg gctagaagat gacaaataat gcatttatga
     7021 atagacgaaa aaggattcaa ttggtttcat ctatcttatt tattttgctt ttgattgctg
     7081 ggctgtttta tcctctattt ggatacttcg ctttggtttg catggcagca ggcatgttaa
     7141 tagcaatatt caagggaagg aaatggtgtg atttctgccc aaggggcagc ttttgggact
     7201 atttcattaa acctttccat aggcgcattc tttattttct ttctgatagt tacgagtgca
     7261 gtagggcttc tgcttggcct gtttgtccag catagaacct ggtgcaggtt ttgccctgtg
     7321 ggaagcatgg gtaacctaat cggcagaggg aagtatcagc taaagattga ctcaaaaaaa
     7381 tgcacggact gcggcatttg ctccaaagtc tgcccgatgc agttaaaccc taacagccat
     7441 aaaaaaggct ctgttgatat tgtaagggag cctgactgca taaagtgcag gctttgtgtc
     7501 aattcatgtc caagcaaggc tttggaattt tgactccggt tagaaagtgc aattatgcta
     7561 atagcagcaa atccttaccc ttactgctaa attcaatcaa aaaaacatca caatatactt
     7621 tattgcagta agagtttgat gaattcacaa tatgcaagga caggctttga tatgctcagg
     7681 agtgaatcaa gaacaatgtt tcttccaaac gagctttggg aggaattact gaatcaaacc
     7741 ctgggcattt gttcagtctc tgagtatgta atgatgggca gaggaggatt ttaatattat
     7801 ctcaaactgg cccctttcaa aatcgccttc ttgtttatct cgacaaa
//
LOCUS       DASRUK010000073         3439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1067894, whole genome shotgun sequence.
ACCESSION   DASRUK010000073 DASRUK010000000
VERSION     DASRUK010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067894"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..963
                     /locus_tag="VFF28_03320"
     CDS             262..963
                     /locus_tag="VFF28_03320"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HZX44691.1"
                     /translation="MAQQQMSEEQVKELQEKIKNMSPEELKEFQKKQCIFCHIISGKV
                     QSKKVYEDDRCLAILDINPANPGHVLLIPREHYTIMPQVPESDLTHLFSVAKTISNAA
                     LRALGVQGTNIIVQNGIAAGQKAQHFMVHVIPRKENDGIKFELPQKEIPEKELEQVRQ
                     KLQSKLGPVKQEKPKVDIQKLVGIKSQEKQEKSRQEDEQADIPKEAKKESIKALKKKN
                     SGVDLDDIAKVLSGR"
     gene            953..1468
                     /locus_tag="VFF28_03325"
     CDS             953..1468
                     /locus_tag="VFF28_03325"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HZX44692.1"
                     /translation="MADKCVICDIVQARIPSKKIYEDEEVIAVLDYNGANPGHSFIIP
                     KDHFPILEQVPANLVGKIFNVANKVSSAIFDTLKVEGTNIFVANGIPAGQKVAHFMVS
                     VIPRKEGDGLNLNWQPRQLSEEEMSTIELKMKEEIGSIGLPQEPKPAQQEVKHIFSEE
                     NYLAKQLRRIP"
     gene            complement(1465..2544)
                     /locus_tag="VFF28_03330"
     CDS             complement(1465..2544)
                     /locus_tag="VFF28_03330"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HZX44693.1"
                     /translation="MAKATIQLSVRWIVIIIILLALLFLLLSFIQRTMRISMASIEEK
                     IVEGAPKPVVEITAPENMAIFEVGEEVNFESYAYSYSENVKITDYYWDYNTDGYVDSR
                     LKDPTTIYYEPGDYNVTLKVLSSIGGLGTDSIIVRVFTKNKKKMNKYDGNPAFFIPVV
                     PSTDSSLNVENWKEIIQVIPLTRWNDKYGDHSYEYVAIAKGEGRGEITKSDVGDKLSS
                     MGKTHGVTFDMGTITPDGYTVSNEYTSDLANYFSYWEEYDSVVVVDEDNAAGALIAGL
                     FAAYYNSPIVFIDSRLMGASYEDYEINGKRIYVIDNIPSSAFDYFITKGNALSWQPYS
                     SDELRDPSRKINRIVELKSNLTIKG"
     gene            complement(2811..>3439)
                     /locus_tag="VFF28_03335"
     CDS             complement(2811..>3439)
                     /locus_tag="VFF28_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44694.1"
                     /translation="TWCYRENESIEVQKCNDAGALLPEGLTCSQKDQVCNGDPDLNIQ
                     TCAENLSVSTSALQCHNHKKQCYSELAPYYYSGIMGGNGGCCGDDGLNECWLDSNANA
                     CCYYKNASAVAYLTDPDDSMGYCLLLGYNKTKVSAGACDNGDTYCWDEVSQNCCGDEM
                     NETWSYSTSRKIGDVLVNETCYNGRWTLISKKETTYFDLMSKILDMLG"
BASE COUNT         1052 a          761 c          707 g          919 t
ORIGIN      
        1 ctctagggat cggattgttc atcgtctttg tctatcacgt tgttttggac atattaaata
       61 gaagagcgac gctttagggt atacccctca ttgaatccca gccaagatgc tattgtgaag
      121 ggtgtgagga accatcaata aactcatcaa tagcaactgg cgtctattcc ccgtactgag
      181 aaaaaattaa taatggattt acaaactttc attgatttaa ataacaacct ttatataaaa
      241 aaataattct ctttaccgat tatggctcag cagcagatgt cagaagagca ggtaaaagag
      301 cttcaggaaa agataaaaaa catgtcgcct gaagaattaa aagagtttca aaagaagcaa
      361 tgtatcttct gccatatcat ctcaggaaaa gtccagtcaa agaaggtcta tgaagatgat
      421 aggtgcctgg ctatcctgga tataaatcct gcaaatcccg gccatgttct tcttatccca
      481 agagagcatt acacgattat gcctcaggtg ccagaatcag acctcacgca tttattctca
      541 gttgcaaaaa ccatctcaaa tgcagcctta agggcgttag gtgtccaggg aacaaatatt
      601 atcgtccaga atggaatagc cgcaggccaa aaagcccagc acttcatggt acatgtcatc
      661 ccaagaaaag agaacgacgg cataaaattt gagcttcccc aaaaagagat tccagaaaaa
      721 gagctggagc aggtaaggca aaagctccag tcaaagcttg ggccagtaaa gcaggaaaag
      781 ccaaaagttg atattcaaaa gctggtaggg ataaaaagcc aggaaaagca agaaaaatca
      841 cgacaagaag acgaacaagc agacatacca aaagaagcta aaaaagaaag cataaaagca
      901 ttaaaaaaga agaactcagg cgtagactta gacgatatcg ccaaggtgct cagtggcaga
      961 taaatgtgtg atttgcgaca tagtgcaggc aaggatccca agcaagaaga tatatgagga
     1021 tgaagaagtc atagctgtat tggactataa cggcgcaaat ccaggccatt cattcatcat
     1081 accaaaagat catttcccta tcttggaaca agttcctgca aatttggtag gaaagatatt
     1141 caacgtcgca aacaaggttt cctcagctat ctttgacacg ctaaaagtag agggcacaaa
     1201 catctttgtg gcaaacggta tccctgcagg acaaaaagtt gctcatttca tggtaagtgt
     1261 catcccaaga aaagaaggcg acggcctaaa ccttaactgg cagccaagac agctctcaga
     1321 agaagagatg tcgacaatag agctaaagat gaaagaagag ataggaagta tcggcctgcc
     1381 tcaagagcca aagcctgccc agcaggaagt aaagcacatt tttagcgaag aaaattatct
     1441 ggcaaagcag ctgagaagga taccttaacc ttttatggta aggttggact ttagctctac
     1501 tatcctgttt atctttcttg aagggtctct tagctcatcc gaggaatatg gctgccagct
     1561 gagcgcattg cctttcgtga tgaaatagtc aaatgcggat gacgggatgt tgtcgatgac
     1621 ataaattctt ttcccgttaa tctcataatc ttcatacgat gcacccataa gcctgctgtc
     1681 gataaaaaca atgggggagt tgtaataggc tgcaaaaagc cctgctatca atgcgcctgc
     1741 tgcattatcc tcatctacaa caacaacgct gtcatattcc tcccaatacg aaaaatagtt
     1801 agcaaggtcg cttgtatact cattgctgac agtatatcca tcaggggtga tagtgcccat
     1861 gtcaaaggta actccatgtg tttttcccat tgagctaagc ttgtctccaa catcactttt
     1921 tgttatttct cccctcccct ccccctttgc aatagctacg tactcatatg agtgatcccc
     1981 atatttatca ttccatcttg tcaaagggat tacctggatt atctctttcc agttctccac
     2041 gttcaaagac gaatcagtgc ttgggaccac aggtataaaa aaagcagggt ttccatcata
     2101 tttgttcatc ttcttcttgt tctttgtaaa cacccgcaca atgatgctgt cagtgccaag
     2161 gcctccgata ctgctaagga ccttaagagt cacattatag tcccctggct cataatatat
     2221 ggttgtcggg tccttaagcc tactatctac atacccgtcg gtgttatagt cccagtaata
     2281 atcagttatc ttgacattct ccgaatatga ataagcatac gattcaaaat taacctcctc
     2341 ccctacttca aaaatagcca tgttctcagg agcagttatc tcaacgaccg gctttggcgc
     2401 accctcaact atcttctcct ctatgctagc cattgaaatc ctcatggttc tttggataaa
     2461 agaaaggagc aaaaaaagca gtgcaagaag gattatgatt atcactatcc accttacaga
     2521 aagttggatg gttgcttttg ccattgtcaa gaaccgccag tcatagacac tgagggaatg
     2581 cagctgtgag gctcatagca tggcggttct tgagcatgaa ctgaaaaatt tgcagagcaa
     2641 atttttggtt gcctaagaaa tttatgcgaa aatttcttgg gcgcaagaaa tgcaatcgca
     2701 tttctttgcg tgttggaaat gcaagatttc caacactcag aaattttctt ccttgcctgc
     2761 ggctcccttt aggccaacag tcatagattg aatggaaaat ttctgacatt ttagccaagc
     2821 atatccagta tctttgacat aaggtcaaag taagttgtct cttttttgct gatgagagtc
     2881 cagcggccgt tatagcatgt ctcgttgaca agaacatctc ctatcttcct gctggtagaa
     2941 tagctccatg tctcgttcat ttcatctcca cagcaattct gtgacacttc gtcccagcaa
     3001 taggtatctc cattgtcaca tgctcctgca gaaacctttg ttttgttata ccccagaagc
     3061 aggcagtatc ccatagagtc atctgggtct gtaaggtaag ctacagcaga ggcgtttttg
     3121 taatagcagc aggcatttgc atttgagtca agccagcatt cattaaggcc gtcgtccccg
     3181 cagcatcctc cattgccgcc cattataccg ctgtaatagt aaggtgcaag ttcagagtag
     3241 cattgtttct tgtggttatg gcactgtaat gcgcttgttg agacggatag attttcagcg
     3301 caggtctgga tgtttagatc aggatcgcca ttgcaaacct gatccttctg tgaacaggtc
     3361 aaaccttcgg gaaggagagc ccctgcatca ttgcacttct gcacctcgat gctctcattt
     3421 tctctatagc accaagttc
//
LOCUS       DASRUK010000074         2600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1727098, whole genome shotgun sequence.
ACCESSION   DASRUK010000074 DASRUK010000000
VERSION     DASRUK010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1727098"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="VFF28_03340"
     CDS             complement(<1..992)
                     /locus_tag="VFF28_03340"
                     /EC_number="2.8.4.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase"
                     /protein_id="HZX44695.1"
                     /translation="MTQIYFQTHGCSTNQSESEVMMGLLADARFLIVQDAGEADILIL
                     NICTVKGEETALREIRKTSEAYPKKKLIVAGCLTKTILREARKIREDVNFISTHNIKS
                     IVEVVEEILFGNVIEATGADSYKKINLPKLRSNSVVGIVPILSGCANYCSYCSVKYVK
                     GKLLSYDIEDIEKEMAYCVDSGCKEIWVTSQDNAAYMLEREKISKLPQLINKLTAIEG
                     KYFIRIGMMNPGHLLDILEPMADAFKSDKVFKFLHVPVQSGNDDILNLMKRNYRLADF
                     KKIVEVFRKRVPNLTLSTDIIAGFPTETEQQFNDSLELMKEIKPDVLNISRFQP"
     gene            complement(1035..1376)
                     /locus_tag="VFF28_03345"
     CDS             complement(1035..1376)
                     /locus_tag="VFF28_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44696.1"
                     /translation="MTDIAQTMQGKKPLAIASSIAALIIAIAARQGIQNSREEPQSAK
                     GVVRGMVPYSHADGKGYIVCIEGLHGSVKIPINRWDETVNQGDPVCLTYRRHHSFVDP
                     PYRALSIMDNK"
     gene            complement(1419..1838)
                     /locus_tag="VFF28_03350"
     CDS             complement(1419..1838)
                     /locus_tag="VFF28_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44697.1"
                     /translation="MKERKSLRAAPALTFFEAKEEKKVNEKPYQLDITYEQGKTIEKL
                     SIKYSLRDYPFEREKALQDFTGILDKKLNIAPSSTMGGILGYAYLGENFMVRRDDVHG
                     DKALMVDVHEAIHTHDEYETRVLTSWMLARERARYKK"
     gene            complement(2059..2190)
                     /locus_tag="VFF28_03355"
     CDS             complement(2059..2190)
                     /locus_tag="VFF28_03355"
                     /inference="COORDINATES: protein motif:HMM:NF019876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HZX44698.1"
                     /translation="MADEASLQKVEGQGTKKCPECGSEKIIREDDEIYCEKCGFVID"
     gene            complement(2272..>2600)
                     /locus_tag="VFF28_03360"
     CDS             complement(2272..>2600)
                     /locus_tag="VFF28_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44699.1"
                     /translation="LMLLKILNDEHKTLKQKISEFPKYHTLQRKIAAKGYSKDKVKRH
                     YKGFGFKETGDETGGLKVILDEKSFVWFRVSKTEPDTLRIISESESEEKSQEMMSQAI
                     TALQKE"
BASE COUNT          642 a          619 c          461 g          878 t
ORIGIN      
        1 cttggctgaa accttgagat attaagtaca tcgggtttta tctctttcat aagctccaag
       61 gaatcattaa actgctgttc agtctctgtc ggaaaccccg cgatgatgtc agtgctgagc
      121 gtaaggttgg gaaccctttt cctgaagacc tcaacaatct tcttaaaatc cgcaagtcta
      181 taattccttt tcatcaggtt tagtatatca tcatttcctg actgcacagg cacatgcaga
      241 aacttaaaca ccttatcgct cttaaaagca tcagccatgg gctcgagtat atccagtaga
      301 tgccctggat tcatcatccc tatccggata aagtactttc cttcgattgc agtcagctta
      361 ttgattagtt gaggcagctt gcttatcttc tctctttcaa gcatatatgc ggcattatcc
      421 tgcgatgtca cccatatctc tttgcatccg ctgtcaacgc agtaagccat ctccttctca
      481 atatcctcaa tatcatagga tagcagcttt cccttgacat atttgacaga acaatagctg
      541 caatagtttg cgcagccgga aagtatgggt actattccca cgacagaatt gctcctgagt
      601 ttgggaagat taatcttttt atagctgtca gcgcctgtcg cctcgataac gtttccaaac
      661 agtatctcct ctacaacctc cactatggat tttatattat gggtgctgat aaaattgaca
      721 tcttctctta tcttgcgagc ttcccttaag atagtttttg tcaaacaccc agcgacgata
      781 agcttttttt taggataggc ttcgcttgtc tttcgtatct ccctaagtgc agtctcctca
      841 cccttgacag tgcatatatt taatattaag atatcagcct cgcctgcatc ctggactatg
      901 agaaaccttg catcagcaag cagtcccatc atgacttctg attcgctttg gttggtgctg
      961 caaccatgcg tttggaaata gatttgggtc atgagaaaga aagttattat tttattaata
     1021 aaggttagct aaaatcattt gttatccatt atagaaagcg ctcgatatgg tgggtcaaca
     1081 aatgaatgat gtcttctata agttaaacac actgggtcac cctggttaac tgtctcatcc
     1141 catcgattga taggaatttt caccgaccca tgcaaccctt ctatacagac tatgtatcct
     1201 tttccatctg catggctgta agggaccatg cccctgacga ctccttttgc gctttgcggt
     1261 tcttctctag agttttgaat tccttgccgg gccgcaatgg cgatgattaa agctgctatg
     1321 ctactggcta ttgctaaagg ttttttccct tgcatggttt gggctatgtc agtcatacag
     1381 tctgatttat tggtcactat ttaaatttag ctgatgagtc atttcttata cctcgccctc
     1441 tcccttgcaa gcatccagct tgtcagaacc ctagtctcgt attcatcgtg ggtatgtata
     1501 gcctcatgta catcgaccat cagcgctttg tcgccatgca catcatccct tcttaccatg
     1561 aagttctcgc caagataagc atagccaagt atacctccca tcgtagaaga aggtgctata
     1621 ttcagttttt tatcaagtat gccagtaaag tcctgcaagg ccttctctcg ctcaaaagga
     1681 taatcgcgaa gtgaatactt tatgcttaac ttctctatag tcttcccctg ctcgtaggtg
     1741 atgtccagct ggtaaggctt ctcattgacc ttcttctcct cttttgcttc aaaaaaagta
     1801 agcgccggag ctgcacgtaa gctttttctc tctttcattc tcgctgtact tgactgtaaa
     1861 tggggtctct tgtctggttc gttcgaaaga aaaaagaaaa tccgattttt tagccttgat
     1921 ttcatggatt atctgctaaa ggtagctagc cttcttttcg ctaaaagccc tttttgccag
     1981 ctctccaagt ataggctgtt ttttaggctt gtctagaact atatatctaa ggatttgctt
     2041 gattaaatca tccatagttt aatcaataac aaaaccgcat ttctcacagt agatctcatc
     2101 gtcttctctg atgatcttct cgctgccgca ttcagggcat ttctttgttc cctgcccttc
     2161 aaccttttgt aatgatgctt cgtcagccat gtttaccaaa actcgctaat taaatcttga
     2221 cttccaaacc accctttttc aggagatatg catgaagatt tatttataaa tctattcttt
     2281 ttgtagggca gttatcgcct gggacatcat ctcctggctc ttttcctctg actctgactc
     2341 tgagattatc ctcaaagtgt ctggctcggt ctttgaaacc ctgaaccaga caaagctctt
     2401 ttcatccaag attaccttaa gccctccagt ctcatcacct gtctctttaa agccaaatcc
     2461 cttatagtgc ctcttcactt tatcctttga atatcccttt gctgctatct ttctttgcaa
     2521 agtatgatat ttgggaaact cgcttatttt ctgcttaagg gttttatgtt catcatttaa
     2581 tatctttaga agcataagaa
//
LOCUS       DASRUK010000075         3201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_64900, whole genome shotgun sequence.
ACCESSION   DASRUK010000075 DASRUK010000000
VERSION     DASRUK010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64900"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..1598)
                     /locus_tag="VFF28_03365"
     CDS             complement(240..1598)
                     /locus_tag="VFF28_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44700.1"
                     /translation="MKRLYAIGLFLIVMLAASMSAAVLMDKTSIDITSKPTGPISSTF
                     KLTNNNTASTTASLQLVSSDLSRYNLSLSPNGFNQQFTMSPGQSIDFTLTGRVPKDVT
                     TRTAAYTGTIKAYFGTTELASLPLNVIVESQLSLEDVKFKVDGKSRSIDNGNTRSDVF
                     PGSKLEISGDVENLFSDDDDIQIEDVEVEITIEGIDDDDDLEETIEIGDIDADDTESF
                     TATFDIPENVDEGDYDVRIEVTGDDENGGKHVVLWDDIKIDIEKEKHDIQIRKLSLAP
                     TRVDCNRNIKLDMVLKNLGRTDEDEVVLRIENTALGISEEDTSIPEIEEGTDDETEYE
                     KVYSFRIGDSVRAGTYPISVKAYYETDTLSDTQSVDLTVAECRADEPVKRNDSVVIVN
                     PPRRDEPSEPDVIIEPVDDTEETGILDSSGYMIFLIAAIGVAVLVIIVMIIVLFTVRR
                     KD"
     gene            1839..2258
                     /locus_tag="VFF28_03370"
     CDS             1839..2258
                     /locus_tag="VFF28_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44701.1"
                     /translation="MEKLARRMKKGLLDFLNVSLSPFSSIAIASGVEADIKGLAKKMH
                     PQQFFALLRGHVKNSKLVIDGLYYQPFDPLAKTAYITFEPPYMPDMVGSVHSHPTPEP
                     KLSQADLGFFGKKGGIHLIISYPYDSIDRYGSKGEKL"
     gene            complement(2255..2740)
                     /locus_tag="VFF28_03375"
     CDS             complement(2255..2740)
                     /locus_tag="VFF28_03375"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HZX44702.1"
                     /translation="MTELTDPRQVILVTSREEMDILGKKEIKDNIITLAWHMPTSFDP
                     LLYAISVGKTRFTSEIIKRSRVFVVNFVSEKLADKAMLIGTKSGRHIDKFKESGLTKE
                     EADSIDCCRIREAAAYAECEVVNEIETGDHTLFIGKVLSSKVKTDGKRLLQKNKSFIG
                     A"
     gene            complement(2746..>3201)
                     /locus_tag="VFF28_03380"
     CDS             complement(2746..>3201)
                     /locus_tag="VFF28_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZX44703.1"
                     /translation="YEELHKEEQLEKIALIKKYLKVNASDKLLDVGCGTGLTTEPWEC
                     RRYGIDPAPKLLARARQRDKIEYTLAQAESIPYPDSFFDIVISITAIQNFSDIKKGLS
                     EIKRVGKEKFALSFLRKSTQRGIFEILIRKIFKVENVISEKKDLIFFCR"
BASE COUNT          871 a          734 c          581 g         1015 t
ORIGIN      
        1 attcctgaaa tggaaagggc aattatgaaa ttcttcagaa gtctttggta caacaaacaa
       61 aaagttctat ctcttattcc atttaatgag aaatattcaa tttaatttac tataatggcc
      121 ccttcaaacg tatatactag cagaaagccc ttgtcaagga agcgctaaaa aaaagaggcc
      181 taagccgatt attctaagac aacaaaagat atatctaaaa taagaaaaaa aagaataatt
      241 taatcttttc ttcttacagt aaacaagacg ataatcatca cgattatcac aagcactgca
      301 acacctattg ctgctatcaa aaatatcata tagccgcttg agtcaagtat gcctgtctct
      361 tcagtgtcgt ctactggctc aattatcaca tcaggctcgc taggctcatc tctcctggga
      421 ggattgacga taacaactga gtcgttcctc tttacaggct catcagctct gcactctgcg
      481 acagttagat cgacagattg ggtgtcagac aatgtgtctg tttcatagta ggccttgact
      541 gatatcggat aagtgccagc tcgaacagaa tctcctatcc tgaatgaata aactttttca
      601 tactcagtct catcatcggt gccttcttct atttcaggta tggatgtatc ctcttcagat
      661 atgccaagtg ctgtgttctc tatccttaag acaacctcat cttcatcagt cctgccaagg
      721 ttctttagca ccatatcaag ctttatgttc ctgttgcaat cgactcttgt aggagcaagg
      781 gacaacttcc ttatctggat gtcgtgcttc tccttttcga tatcgatctt tatatcatcc
      841 cataagacaa cgtgtttgcc gccgttctca tcatcaccgg tgacttctat ccttacatca
      901 taatcccctt catcaacatt ttcagggata tcaaaggtgg ctgtgaagct ttcggtatca
      961 tcagcatcaa tgtccccgat ctctattgtt tcctcaagat catcgtcatc atctatgcct
     1021 tcgatggtaa tttctacttc tacatcctct atctggatat catcgtcatc agaaaaaagg
     1081 ttctctacat ctcctgagat ctcaagtttt gatccaggaa aaacatcact tcttgtattt
     1141 ccattgtcta tgctcctgct cttgccatct accttgaatt ttacatcctc aaggcttagc
     1201 tggctctcga caatgacatt caaaggaagt gatgcaagct ctgttgtgcc gaaataagct
     1261 ttgattgtgc ctgtataagc agctgtcctt gtagtaacat ccttaggcac tctgccagta
     1321 agggtgaaat caatgctctg gccagggctc atggtgaact gctggttgaa gccattagga
     1381 ctcaggctta ggttatacct ggaaaggtct gagctcacaa gctgcaaaga tgcagttgtt
     1441 gaagctgtat tgttgtttgt aagcttaaat gtgctggaga ttggtccagt tggcttagac
     1501 gtaatatcaa tagaagtctt gtccatcaat actgctgcac tcatgcttgc agcaagcatc
     1561 actatcaaaa acaaaccgat tgcatatagt cttttcattt tatgaccccc atatttttta
     1621 tggttgatgt aaagtatgga gtactttact acattaggaa aaagcgatgc ttaatcctat
     1681 ttattattag agagtggtat catgttatat ttaaagttta ctattctaaa gtatacataa
     1741 tcacaccact gccattaagg tagcaagtgc aaatttatat gctttgctat agcttcaaac
     1801 cggtaatctt ataagctaag acagctttat gcctttccat ggaaaaactg gccaggagga
     1861 tgaaaaaagg tcttctggat ttccttaatg ttagcctttc tcctttctcc agtatcgcga
     1921 tagcatcggg ggtcgaggct gacatcaaag gccttgcaaa aaagatgcat ccacagcagt
     1981 tctttgccct gttaagaggc catgttaaaa acagcaagct ggtcatcgat ggcctttatt
     2041 accagccttt tgatcctctg gcaaagactg cctatatcac ctttgagccg ccatatatgc
     2101 ctgacatggt cggctcggtc catagccacc cgacacctga acctaagctt tcacaagcag
     2161 acttaggttt ctttggaaaa aaaggaggca tccacctgat aataagctat ccctatgatt
     2221 ctatcgacag atatggttcc aagggagaga agctctaagc tccgataaag ctcttgttct
     2281 tctgcaaaag ccgctttcca tcagttttca cttttgagct aagcactttt cctatgaata
     2341 gggtatgatc ccctgtttct atctcattaa caacttcaca ttcggcgtaa gctgctgcct
     2401 cccgtatcct gcagcaatcg attgagtctg cctcttcttt ggtaagccca gattccttaa
     2461 acttgtctat atgcctgccg gactttgttc cgattagcat tgctttgtca gcgagctttt
     2521 cagagacaaa attgaccaca aaaactctgc tcctctttat gatctcagac gtaaaccttg
     2581 ttttgccgac agatatggca taaagcagcg gatcaaagct tgtaggcata tgccaagcaa
     2641 gcgttattat attatctttt atctcctttt ttccgaggat gtccatctct tctcgtgagg
     2701 ttactaggat aacctgcctt ggatcagtaa gttctgtcat tacaatcacc tgcaaaaaaa
     2761 tattaaatct ttcttttctg atataacatt ttcaacctta aatatttttc ttatcaggat
     2821 ctcgaaaatc cctctctgag tgctttttct caaaaagctt aatgcaaatt tctcctttcc
     2881 cacccgcttt atctcagaaa gccctttctt gatgtctgaa aagttctgga ttgcagtgat
     2941 gctgatgact atatcaaaga atgagtccgg ataaggtatg ctttcagcct gcgcaagcgt
     3001 gtattctatc ttatcccttt gccgcgccct tgcaagaagc ttcggcgcag gatcgatgcc
     3061 atagcgtcgg cattcccatg gctctgtcgt aagccctgtt ccgcagccta catcaagaag
     3121 cttatcagaa gcatttacct taaggtattt ttttatcaag gctatctttt caagctgctc
     3181 ttctttatga agctcttcat a
//
LOCUS       DASRUK010000076         3902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_65923, whole genome shotgun sequence.
ACCESSION   DASRUK010000076 DASRUK010000000
VERSION     DASRUK010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65923"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..1218
                     /locus_tag="VFF28_03385"
     CDS             190..1218
                     /locus_tag="VFF28_03385"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HZX44704.1"
                     /translation="MLNEKDVSRIKEELDNCKNPLFFFHDDPDGLSSFLLLYRYIREG
                     HGIVFKAKPGLSAKFLPKVAEYSPDKIFILDIPLVDQEFIDGAKTPVIWIDHHGPYVR
                     TGVKYFNPRIKDKNDGTPVTRICYEVVKQDMWIAAVGSIGDWHIPDFFEEFTSKYPGF
                     ADKKVKNPGELYFDSKLGKLVRIFSYILKGKMTDANKCFKILTRINDPNEILDRGSSR
                     GSFLVKRFEKIEKEYQNLLASAVKEADKDRFLVFIYPEDKMSFTGDLSNELIYRYPDK
                     IIIVGREKNGEVRISIRSTNVRLPPVIEKAVMGCEGYGGGHEYACGANIKKEDFPKFV
                     ENIKQQIS"
     gene            1303..2184
                     /locus_tag="VFF28_03390"
     CDS             1303..2184
                     /locus_tag="VFF28_03390"
                     /inference="COORDINATES: protein motif:HMM:NF021504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HZX44705.1"
                     /translation="MTIVGIPNTLFNFKHPVLWSTFFKELGVKTIVSTDTNLKILDES
                     VKLSDSECCLSVKLLAGHTRALIGKCDFIFVPTLSSEKKGYLSCPRFKAVPHLAKIFT
                     NGDTRILTADYNRNYHSKAYFLFKLGIRLNHNPWRVYKAVKKAISCEDMDCTSRTKRF
                     GTKAAKAGKKILIISHPYILADKFMNHSIVSKLEGMGAVPIPIDEVPFEEKSTEIKWD
                     FAKEVLNCIDYALDNKTFDGIIQITNFNCGCDSVTVDIIRDKIKNHDVSYLNLIIDEH
                     TAEAGIETRLEAFIDSL"
     gene            2181..3191
                     /locus_tag="VFF28_03395"
     CDS             2181..3191
                     /locus_tag="VFF28_03395"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HZX44706.1"
                     /translation="MKRYTIPHFGDYTIALKALIDPDAEFVITTPTHRTIELGVRHSP
                     EFICYPYKIVLGNMIENINLGATDLFMLDINASPFEAECRLAYYSLLQKMFLNEHKVA
                     FHRLRIEKIVQSLKKELPDCSLFKIVNNIMLFALKTYLIDSLNTAYYRTISEGKKKVK
                     DEILREIDMTNKAFGLLKVKKKIKKSFPQSKGRLRVAIIGEIYLIHETSINNDIAGIL
                     NDLGVEVDVLANLSHFILYSFCKKRMIKKYAKTSKEYLDCAVGGHGLDSVAHMIESGK
                     YAGIIQILPFGCMPETSVRPILSKVASDKKINYLSLSLDQHAGKAGLETRLEAFVDMI
                     RK"
     gene            3188..>3902
                     /locus_tag="VFF28_03400"
     CDS             3188..>3902
                     /locus_tag="VFF28_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HZX44707.1"
                     /translation="MNKTYLGVDVGSISTNVVLLCGDRVIHSSYIRTSGNPARSVKEG
                     LIEVKSKFGEIEIAGVGTTGSARKLIGELIGADIVKNEITAHAMASTFYCPNVSTIIE
                     IGGQDSKLIILKNGTVVDFCMNSVCAAGTGSFLDSQANRLRIPIEEFGGISLNSKDGV
                     NIAGRCTVFAESDMIHKQQIGHKIEDILYGLCKSLVRNYLNNLSRGRELYEPIMFQGG
                     VSENKGIIRAFEESLGKKLI"
BASE COUNT         1257 a          722 c          877 g         1046 t
ORIGIN      
        1 ttccaccact tcaaatcctt ccatcttcat ttggattggc tttttcattg tagttacaat
       61 gtaactacat ctgatatatt aatttttcta tactctagta tataagttgt aaaaatcaag
      121 aaaagtcagc ttaccttata cccattttac aaaagcttaa atagcttgac tttattgaat
      181 tcttttactg tgctaaatga aaaggatgtt tccaggataa aagaagagct tgacaactgt
      241 aaaaatccgc tgttcttttt tcatgacgac cctgacgggc tcagttcctt tctcctatta
      301 taccgatata tacgagaagg ccatggcata gtatttaaag ctaaacctgg gctttcagca
      361 aagtttttgc ctaaagtcgc tgaatattcc cctgacaaga tatttatcct tgacataccc
      421 ctggtcgacc aggaatttat cgatggagca aagactcctg ttatctggat agaccatcat
      481 ggaccttacg tccgaacagg agtcaagtat ttcaatccca gaatcaagga caagaatgat
      541 ggaactcctg tcacaaggat atgctatgaa gtggtaaagc aggatatgtg gatagctgcg
      601 gtcggcagca tcggtgactg gcacatccct gatttttttg aggaatttac atcaaaatat
      661 ccaggttttg ccgataaaaa agtgaagaat ccgggtgagc tatattttga ttccaagctt
      721 ggaaagcttg tgaggatatt ctcatatatt cttaagggaa agatgaccga tgccaacaag
      781 tgctttaaaa tattgacacg gataaatgac cctaatgaaa tattggacag gggctcttcg
      841 agaggatctt ttcttgtcaa acgatttgaa aagatagaga aagaatatca gaacctgctg
      901 gcttctgccg taaaagaagc tgacaaagac agatttcttg tctttatata tcctgaggac
      961 aagatgagct ttacaggaga cctttcaaac gaacttatct accgctatcc tgacaagata
     1021 atcatcgtcg gcagagagaa gaacggcgaa gtgaggataa gcataagaag caccaatgta
     1081 agattacctc ctgtcatcga gaaagctgtc atggggtgtg aaggatatgg cggagggcat
     1141 gagtatgcct gcggagcaaa catcaagaaa gaggattttc ctaagtttgt agagaatata
     1201 aagcagcaga tttcttagac atctaataag aaagtatttg ccaaacgatc aattgccgag
     1261 cgcttgaata agtttataaa tgatatctga caaccgcaaa acatgactat tgtggggata
     1321 ccaaacacgc tgtttaattt caagcatcct gtattatggt caacgttctt taaagagctg
     1381 ggagtcaaga caatagtatc aacagataca aatcttaaga tacttgacga atcagtcaaa
     1441 ttgagcgaca gcgaatgctg cctttctgtc aaactccttg cagggcatac aagggctctt
     1501 atcgggaaat gcgactttat ctttgttcca accctttctt cagaaaagaa gggctatctg
     1561 tcatgcccga ggtttaaagc tgtgcctcat ctggccaaga tatttacaaa tggggacaca
     1621 aggatattga ctgcggatta taacaggaat tatcattcaa aggcttattt tctttttaaa
     1681 ctcggcataa gattgaacca caatccctgg cgagtctata aggctgtaaa gaaagcgata
     1741 agctgtgaag atatggactg cacaagcagg accaaaagat ttggcaccaa ggcagcaaag
     1801 gctggaaaaa agatcctcat aatatcccat ccatatatcc ttgctgacaa gttcatgaac
     1861 catagcatcg tctctaagct tgaaggcatg ggggcagttc caataccaat agatgaagtt
     1921 ccttttgaag aaaagagcac tgagataaaa tgggattttg ccaaggaagt gttgaactgc
     1981 atagactatg cccttgataa caagacgttt gatggaatca tccagataac caattttaac
     2041 tgcggatgcg actctgtcac tgtcgacata ataagggata aaataaagaa ccatgacgta
     2101 agctacctta acctgataat cgatgagcat actgctgagg cagggataga aacaaggctt
     2161 gaggcattta tcgactcctt atgaaaagat acaccatacc tcattttggg gattatacaa
     2221 tagcattaaa ggctcttatc gatcctgatg ctgagtttgt gataacaact ccaacgcaca
     2281 ggactatcga gcttggtgtg aggcattctc ctgagttcat ctgctatcct tacaagatag
     2341 tgcttggaaa catgattgaa aatatcaatc tgggtgccac tgacctgttc atgctggaca
     2401 tcaacgcctc tcccttcgag gctgaatgca ggcttgctta ctattcgctc cttcaaaaga
     2461 tgttcctgaa tgagcataag gtggcgtttc ataggctgag gatagaaaaa atagtacagt
     2521 cactaaagaa ggaactgcct gattgcagct tgtttaagat agtaaacaat atcatgcttt
     2581 ttgcattgaa gacttacctg atagattctt tgaacacggc ttactatagg acaatatctg
     2641 aaggcaagaa aaaagtgaag gatgagatat taagagagat agacatgaca aacaaagcct
     2701 tcgggctctt aaaggtgaag aaaaagataa agaagtcttt tcctcaaagc aagggcaggc
     2761 ttagggtcgc tataatcgga gagatatatc ttatccatga gacctcgata aataatgata
     2821 ttgcaggcat attgaacgat cttggggtgg aggtagatgt gcttgcaaac ctttctcatt
     2881 ttatcctcta tagcttctgt aagaagcgca tgataaaaaa gtatgcaaag acgtccaaag
     2941 aatatctgga ctgtgctgtt ggagggcatg ggcttgattc ggttgcgcat atgatagagt
     3001 cggggaaata tgcaggcata atacagatac tgccctttgg ctgcatgcca gagacaagcg
     3061 ttcggccgat actgtccaag gttgcaagcg ataagaagat aaactattta agcctgtcac
     3121 ttgaccaaca tgcagggaag gctggccttg agacaaggtt agaagctttt gtggatatga
     3181 tacgaaaatg aacaagacat accttggcgt ggatgttggg agcatctcaa caaatgtagt
     3241 cttactttgc ggagatagag tcatacattc ttcttatatc agaacatcgg gaaatcctgc
     3301 aaggtctgtc aaagagggac ttattgaggt caagtcaaaa tttggcgaga tagagatagc
     3361 aggtgtcggg acaacaggaa gcgcaagaaa acttatcggt gagctcatcg gggcagatat
     3421 agtcaaaaat gagataacag cgcatgctat ggcttctact ttttattgcc ctaatgtcag
     3481 cacaataata gaaatagggg ggcaggacag caaactgata atattgaaga acggaacagt
     3541 tgtcgatttt tgcatgaact ctgtctgcgc tgccgggaca ggcagctttc ttgactcgca
     3601 ggcaaacagg cttagaatcc caatcgaaga gtttggtgga atttcactta attccaagga
     3661 tggagtaaat atcgctggaa gatgcactgt ctttgcagaa agcgatatga tacacaagca
     3721 gcagatagga cataagatag aggacatatt atatgggctc tgcaagtcgc ttgtaaggaa
     3781 ttatcttaac aacctatcaa gaggtagaga gctttatgag cctatcatgt ttcaaggagg
     3841 agtgtctgaa aacaaaggga taatacgtgc ttttgaagag tcgcttggga aaaagctgat
     3901 ta
//
LOCUS       DASRUK010000077         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_571761, whole genome shotgun sequence.
ACCESSION   DASRUK010000077 DASRUK010000000
VERSION     DASRUK010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571761"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="VFF28_03405"
     CDS             complement(<1..1085)
                     /locus_tag="VFF28_03405"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX44708.1"
                     /translation="MNKDYFSNIPKFYLFRFVGSLDLTVAIFVLFMLSNNLTMTQVML
                     IESIFLIMVLSLEVPSGVFADLYGRKTALAISLLCASASFIIFGLGSTFWVFLLAQAV
                     LAFSWSLESGADLAFVYDSLIKGKNFPKIYGQSNFITYIAFAISALLSGFLAALLGFR
                     ALFFISAGLFFLGFLVSLRFTEPPIKKKSAKKHYLHMKGALKFAFSSKVVRNLIIYFA
                     VFSAFSSLAWFIFQPYLKDYSTAIIGIAIFIYYVSGGIGSLISGKLMAKVRQEKLLIY
                     LLLIGSASFLVLSFITPLFGIFFIATLSFSIGIRDISVSEEINIHTDSHHRATVVSIQ
                     SMTRGLIQAAFAPLIGYLVDIFSPAAA"
     gene            complement(1109..1894)
                     /locus_tag="VFF28_03410"
     CDS             complement(1109..1894)
                     /locus_tag="VFF28_03410"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HZX44709.1"
                     /translation="MEQTYSLEKYLFVLTIVITVVLFQYLIPTKNLFLDLFTIYIIPI
                     LFISVFFGRLIIEKSFKNNKGALIAMLISYSISTIIASVLAFIIVILLLGFIPGSLYP
                     LARPSPQSGSSQAAIAMVIISLAIVGPAEEYIFRGFVFGALYFVLQRKHWLPISIFSS
                     IVFTVLHAYYYFTFGIASSIFFVQIFSISLAFSIAYFISGGNLLMPALIHGIYDASAF
                     LDFAAEGNIGAIIRSLIFLSGIILAIMVLIIKRPSLRKITSNL"
BASE COUNT          663 a          385 c          476 g          494 t
ORIGIN      
        1 aacgccgcag ccggcgaaaa gatgtcgaca agatagccga tgagaggagc aaatgctgcc
       61 tgtatcaggc cgcgggtcat gctttgtatg gaaacaactg ttgcacgatg atgggagtcg
      121 gtatggatgt ttatctcctc acttactgaa atgtccctga tacctattga aaacgacagg
      181 gttgctataa aaaatattcc aaaaagagga gtgataaacg aaagaaccag gaaagaggcg
      241 cttcctatca gcaaaagata gattaaaagc ttctcctgcc tgaccttggc catcaacttt
      301 cctgaaatga ggcttcctat cccgccagag acatagtaga taaagatggc aataccgata
      361 atagcggtag aataatcctt gagataaggc tgaaagatga accatgccaa ggagctgaaa
      421 gctgaaaaca ccgcaaaata gatgataaga tttctcacga ctttgctgct aaatgcaaac
      481 tttaacgctc ccttcatatg gagataatgc ttttttgcgc ttttcttttt tataggaggc
      541 tcggtaaacc ttagggagac aagaaagccc aggaaaaaca atcctgctga tatgaagaaa
      601 agagccctaa atccaaggag cgctgcgagg aaccctgaaa gaagcgcaga tattgcgaat
      661 gctatgtatg ttatgaagtt tgactgccca tagatctttg gaaagttttt tccctttatc
      721 aatgaatcgt agacaaaagc aagatcggct cctgactcaa gcgaccatga gaacgcaagt
      781 accgcctggg ccaaaagaaa tacccaaaaa gtgctgccta ggccaaatat tataaagctt
      841 gctgaagcgc ataatagtga tatcgcaagc gctgtctttc ttccatacag atcagcaaag
      901 actccgcttg gcacttcaag cgaaaggacc atgatcaaaa agatagattc aatgagcatc
      961 acctgagtca tggtcaggtt gtttgagagc atgaaaagga caaagatagc gactgtcaag
     1021 tcaagcgagc caacaaacct gaagagatag aacttaggga tattgctgaa ataatcttta
     1081 ttcatatcag tatataggaa gcggttgttt ataaattgga ggtaattttc cttaggcttg
     1141 gccttttgat tataagcacc attatcgcca gaattatccc ggatagaaag atgagcgaac
     1201 ggatgattgc gccaatattt ccctctgctg caaagtcgag gaatgcagat gcatcataaa
     1261 tgccgtggat aagtgctggc atgagcaggt ttcctccgga gataaagtag gcgatgctga
     1321 acgcaaggct tatcgaaaag atctggacaa agaatatgct tgaagctatg ccaaaggtga
     1381 agtagtaata ggcatggagg acagtaaaaa ctattgatga aaatatggat atgggcagcc
     1441 agtgttttct ttgcaagaca aaatataatg ctccaaagac aaagcccctg aagatatatt
     1501 cctctgcagg gccaactatg gcaagggaga ttatcaccat ggctatggct gcctgagatg
     1561 agcctgactg ggggcttggc cttgccaaag gatataagct gccaggaata aagcccagga
     1621 gcaagatgac gatgataaat gcaaggacag aggcgataat cgttgagata ctgtaagata
     1681 tgagcatcgc tatcaaggcg cccttgttgt tcttgaagga tttttcaatt atcagcctgc
     1741 caaagaagac actgatgaac aatataggga tgatatagat agtgaacagg tccaggaaga
     1801 gattttttgt cgggataaga tactggaaga ggacaacagt tataacaata gtgaggacaa
     1861 agagatattt ttcaaggctg tatgtctgtt ccatccatag agcgatgatt tctgggttga
     1921 taaatatatg ctggctaaca cagggcactg ttaactcttc ggcatacatg aaatctctat
     1981 caatttcagc agcttatttc ttcccaagtc aagtttga
//
LOCUS       DASRUK010000078         9150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2231557, whole genome shotgun sequence.
ACCESSION   DASRUK010000078 DASRUK010000000
VERSION     DASRUK010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 9150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9150
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2231557"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..1305
                     /gene="hisC"
                     /locus_tag="VFF28_03415"
     CDS             244..1305
                     /gene="hisC"
                     /locus_tag="VFF28_03415"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZX44710.1"
                     /translation="MTNINPRKAVLEMDEYRPPIEGRRGFLRLDFNENTIGCSPKVLS
                     AINKIKEEEIAAYPEYIKFKEKLAKYLKIKPEQLMVSNATDEAIMVLMQTYIEQGDEV
                     IIPVPTFAMFKFYAQLAGAKVREIPYTANLAFPTKKVLDAATKKTKLIILCNPNNPTG
                     TLIDKKDIIRIIEKARSKDCLVLLDEAYHQYSGAEYLDLIPKYDNLIIIRTFSKAYGM
                     GGIRLGYAASNEDVMKNLLKAQSPYSVNSIAVAAGSKAIEDKAYVDWYVGEVKKAREI
                     LYNALKKLKIKSFPTAANFLIVKLPGKAKEIELELKQKGILVRNRSKDPLLNDCIRIG
                     IGTVKQTEQFITALKEALK"
     gene            1302..1457
                     /locus_tag="VFF28_03420"
     CDS             1302..1457
                     /locus_tag="VFF28_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44711.1"
                     /translation="MNNILLFILSLIGLLALYWALIGQWRWNKMIRQSEEKEKVGKDG
                     KDKSDNI"
     gene            1429..2106
                     /locus_tag="VFF28_03425"
     CDS             1429..2106
                     /locus_tag="VFF28_03425"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZX44712.1"
                     /translation="MAKIKAIIFDIDGVLIDVSQSYRMAIKKTAEFFLGKIIEMKDVE
                     MVKNQGINDDVEAAEMLIQQNSMEFRKEVIRKKFNEYYLGREFDGLLRNEKLLIKPAA
                     LAKLKDYKLAILTGRPKQEADFGIKRFKLDKFFKEIVVREDVKQGKPDPEGLNLLLKR
                     LEVSPDEAIYVGDNVSDIRAAANAKMGFIGVIPPGVNKEFTRGLLKTEGAEIILDNVN
                     MITDVVK"
     gene            2103..2678
                     /gene="hisB"
                     /locus_tag="VFF28_03430"
     CDS             2103..2678
                     /gene="hisB"
                     /locus_tag="VFF28_03430"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HZX44713.1"
                     /translation="MIRKTKETRIEIKLGIYGKGISRIDTGINFFDHMLELFSKHGTF
                     DLTIKAKGDLDVDQHHLVEDVGITLGQAFDKALGKRKGINRAGFFAMPMDETLAILAV
                     DIGGRPYLKCDLDFKKEKIGDLDSDLVCDFFQAFSNNLKCNIQIKSWQARTDHHKLEA
                     VFKAFGRALKMACSKDKRLKEAIPSTKGLIQ"
     gene            2769..3365
                     /gene="hisH"
                     /locus_tag="VFF28_03435"
     CDS             2769..3365
                     /gene="hisH"
                     /locus_tag="VFF28_03435"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HZX44714.1"
                     /translation="MIAIVDYGAGNLRSVTNALDFLKAKYNVTDKPKDLIAADKIIFP
                     GVGSFGDCIASLKDKGLFDTLKKETKNKPYLGLCLGLQVLFEGSEESKGVKGLSIFKG
                     QCKRFTKLKVPQIGWNSISLSKKDHILSGIEENSYFYFVHSYYARPQDKSIILTETDY
                     GIKYCSSISQGNIFAFQFHPERSGEVGLSIINNFIKLC"
     gene            3359..3946
                     /locus_tag="VFF28_03440"
     CDS             3359..3946
                     /locus_tag="VFF28_03440"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZX44715.1"
                     /translation="MLKAILFDMDGVLIDSHDAWLERFNASRRHFGFKDISAEDFDRD
                     VWARNFVETVAQYFPGRTVEDVRGFYFNTFRSFKIKRMPGADEALMYIKSKGLKTAVV
                     SNTQTPLVDIILEDIGIRKYFDIIVGGERVKNAKPNPEIVMIACKELDVDCSETIFIG
                     DTIYDKQAAQKAGCKFVGFRYDSGIKNLVDLRGMI"
     gene            3946..4710
                     /gene="hisF"
                     /locus_tag="VFF28_03445"
     CDS             3946..4710
                     /gene="hisF"
                     /locus_tag="VFF28_03445"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HZX44716.1"
                     /translation="MLAKRIIACMDVDAGRVVKGINFVGIKDAGNPIALAKEYNKQGI
                     DELVFLDITASHEKRDIMAELVKKVAEQIFIPFTVGGGLRSIEDIRKILNAGADKVSL
                     NTAAVLNPGLIKEASNKFGRQCIVLAIDAKKNIKMKNGFEVYTYGGRKSTGIDAVEWA
                     KKGEKLGAGEILLTSMDRDGTKQGFDLALTSKISGSVGIPVIASGGVGNIEHFYDAFT
                     KGKADAALAASIFHYKEYSVKEIKDYLAKKGIIVRR"
     gene            4707..5459
                     /locus_tag="VFF28_03450"
     CDS             4707..5459
                     /locus_tag="VFF28_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44717.1"
                     /translation="MKILVLDYDGVIVDSEPEAFAASMNCYAKFKGKDRLFTVWDIKD
                     IDLKYKTLHEKNKALRPYVSTVDHIYAIMEVIERGIKVENDAQFYNFAANINVPSGFK
                     EEFHRERKRFQEEDFDEWMKSTKPFAQALAGLIAAVKRFDKVVIVTANKNELISPVLE
                     KIGLSYDEIIDTYYSKDKYGAVDSIAETYDADYKDIYFVEDSVFWMEKMIKKGVRCFL
                     ATWGYNTEAQRKEADGIGATLLDEKGIVKELR"
     gene            5456..5887
                     /locus_tag="VFF28_03455"
     CDS             5456..5887
                     /locus_tag="VFF28_03455"
                     /inference="COORDINATES: protein motif:HMM:NF019585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44718.1"
                     /translation="MKPNEGRLQTGEHILARVVQNRVPDAKVAISRFDKEDEGSVDII
                     SSTDLRQLQNLEPAVNAIIKAGFRVDAREFAREELTEFDLSRVPAELNKIRIVEIVGF
                     DKRPCKDPHVDNTAKVERFILKKIERAGKDRYKFTFTVTAG"
     gene            complement(6047..6658)
                     /locus_tag="VFF28_03460"
     CDS             complement(6047..6658)
                     /locus_tag="VFF28_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44719.1"
                     /translation="MPYSQIKHYLELFDKPHAETILRVYGRLLPSFAKDQSVPKEVRD
                     NARFIEHALIEHFSEGALEVIALETRDMKPAPENDFKRYQSIACVVREDELIVDIRYE
                     IQHDKRLRQLGKIGYGKLAMNFRVDEEYGQNSREALQDIWPLISSLEGYASMQGRYKR
                     AEGLMHPNGDIQSAIFVFDKGMPILEGRDVLAEFSLREKPISY"
     gene            6744..7424
                     /locus_tag="VFF28_03465"
     CDS             6744..7424
                     /locus_tag="VFF28_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HZX44720.1"
                     /translation="MIFPSIDLMDGKVVQLVQGKKENKKLEIDDVIGVAKKFLQYEIS
                     VIDLDAAMGNGDNLAIIKQLCAKFRCRVGGGIRTVEKAKELIDSGAKKVIIGSSAFTK
                     QGINLPFLKKLVKEIGREMIIIAIDSLKGKIVIKGWKESTGIDTLSVVKELEPYCSEF
                     LYTYVDKEGMMQGTDLSTLKKLKAITKDEITAAGGISSMDEIDELQKIGVNSVLGMAL
                     YTGKIRLP"
     gene            7503..7691
                     /locus_tag="VFF28_03470"
     CDS             7503..7691
                     /locus_tag="VFF28_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44721.1"
                     /translation="MNSIYRTIRNALLASSLALGTYTMPAKADSQAKLNNNTLETAVV
                     VNGGKSPVRNRLLTDFLF"
     gene            7837..8154
                     /locus_tag="VFF28_03475"
     CDS             7837..8154
                     /locus_tag="VFF28_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44722.1"
                     /translation="MFEGKLGVSHTDLMRRLGGYGDVSAYANDKAANLSFFYGRKLGD
                     GLSIGLFQLMEFPFEDKPKYYTELQLNKQLDKRLSLFGRIELTGFKDPVYLFGMSINL
                     ADG"
     gene            8243..8635
                     /locus_tag="VFF28_03480"
     CDS             8243..8635
                     /locus_tag="VFF28_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZX44723.1"
                     /translation="MNPVVHFEFPADDRKRMIDFYEKAFGWRTKQLGEDMGNYVLVET
                     TETENRMVKKPGAINGGLYDRKMGGNASKCPSVVISVDDINKHLDIVKEAGGKIIDKP
                     MEIPGVGWLVSFIDTEGNVLSMMQPVAM"
     gene            8635..9093
                     /locus_tag="VFF28_03485"
     CDS             8635..9093
                     /locus_tag="VFF28_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZX44724.1"
                     /translation="MQKIVPNLWFDDKAEEAVAFYTTTFKNSKILKVTKYGQEGSKIS
                     GKKEGSVMTVNFLIETQEFVALNGGPEFKFNEAVSFIVNCTDQEEIDYFWDKFGKEGE
                     EGVCGWIKDKFSLWWQIVPEGLDDMLLSEKAMKLFLGMKKIEIEELKRAR"
BASE COUNT         3135 a         1543 c         2103 g         2369 t
ORIGIN      
        1 aaaacaggat acaaggttga gttgtatgac tcaataaaca agcaccagtg gagcctaaca
       61 gtagaattag agccaaaaca gagaaagatt ttggaagcga taggtgttgt gtataaaaac
      121 tagggaaaat tcaggtaatt atcttgtcct tggatttaac cgttatattg cggctaaaaa
      181 ccattataat accctaaaaa ctaaagttat ttatagtaat agtctcaaaa caacaatagc
      241 aaaatgacaa acataaatcc aagaaaagca gtgttagaga tggatgagta taggcctccc
      301 atcgagggaa gaagaggatt tctcaggctg gactttaacg aaaatacgat tggctgcagt
      361 ccaaaggtac taagcgctat caataagata aaggaagaag agatagcagc ttacccagaa
      421 tatattaaat tcaaagaaaa gcttgcaaag tatctcaaga ttaagccaga gcagcttatg
      481 gtttcaaacg ctacagacga agctataatg gtccttatgc agacttatat cgaacaaggc
      541 gacgaagtta tcatccccgt acctactttt gccatgttta agttttatgc tcagttagca
      601 ggtgcaaagg ttagagagat tccatataca gccaaccttg catttcctac aaaaaaagtc
      661 ttagatgctg caacaaagaa gaccaaacta atcatactat gcaatccaaa taatccaact
      721 ggtacattga tagacaagaa agatataatc agaattatcg aaaaagcaag gtccaaggat
      781 tgtcttgtcc ttcttgatga agcttatcac cagtattcag gtgcagaata ccttgatctt
      841 atccccaaat atgataacct tatcataatt aggacttttt caaaagcata tggcatgggt
      901 ggcatcagat taggttatgc ggcctcaaac gaggacgtta tgaagaattt gctcaaggcc
      961 caatccccct actctgtaaa ttcaatagcc gttgctgcag gctcaaaagc aatagaagac
     1021 aaagcatatg tcgattggta tgtcggtgag gtaaaaaaag caagagaaat tctttacaat
     1081 gcactaaaaa aactaaaaat taaaagtttt cccacagcag caaatttcct tatagtgaaa
     1141 cttcctggaa aagcaaaaga aattgagcta gagctgaaac aaaaagggat attggtaagg
     1201 aatagaagca aagatccatt gcttaatgat tgcataagga tagggattgg aacagtaaag
     1261 caaactgagc agttcataac cgctcttaag gaggcgttga aatgaataac attcttttat
     1321 tcatcttatc attgataggc cttcttgccc tgtattgggc gcttattggc caatggagat
     1381 ggaacaagat gataaggcag tctgaagaaa aagaaaaggt aggaaaagat ggcaaagata
     1441 aaagcgataa tatttgatat cgatggcgtt cttatcgatg tcagccagtc ctatcgcatg
     1501 gctatcaaaa aaacagcaga gttcttcctt ggtaaaataa tagagatgaa agatgttgag
     1561 atggtaaaaa accaggggat aaatgatgat gttgaagccg cagagatgct tatccaacaa
     1621 aacagtatgg aattcagaaa ggaagtgata agaaaaaaat ttaatgagta ttacctcggc
     1681 agggaatttg acggccttct aagaaatgag aagctgttga taaagcctgc tgcacttgca
     1741 aaactaaaag actataagct ggctatcctg acaggaaggc caaaacaaga agctgatttt
     1801 ggcataaaac gctttaagct ggataagttc ttcaaagaga tagttgtaag agaagacgta
     1861 aagcaaggca agccagatcc cgaaggattg aatttgctct tgaaaagatt ggaagtaagc
     1921 ccagatgagg caatctatgt tggagacaat gtctctgata tcagggcagc tgccaatgca
     1981 aagatgggat tcatcggagt tatcccgcct ggtgtaaata aggagttcac aagagggtta
     2041 ttaaagacag agggagcaga gattatattg gataatgtta atatgataac agatgtggta
     2101 aaatgattag gaaaacaaaa gagacgagaa ttgaaataaa attaggcata tatggcaaag
     2161 gcatatccag gatagataca gggataaatt ttttcgacca tatgcttgag cttttttcaa
     2221 aacatggaac ttttgacctg acaatcaagg caaaaggaga tttggatgta gatcagcacc
     2281 atctggtaga ggacgtggga ataacactgg gccaggcatt tgacaaagcc cttggaaaac
     2341 gcaaaggcat aaacagggca ggcttttttg cgatgcccat ggatgaaaca ctggcaatac
     2401 tagcagttga tataggagga aggccgtacc tgaagtgcga tcttgacttt aagaaagaaa
     2461 agattggaga tttagacagc gatcttgtct gcgatttttt ccaggctttc tcgaacaatc
     2521 tgaagtgcaa tatccagata aaaagctggc aggcaaggac agaccaccac aagcttgagg
     2581 ctgttttcaa agcattcgga agagcattga agatggcatg cagcaaggac aagagactaa
     2641 aagaggcaat acccagcacc aaaggattga tacaatgaaa tatagccaat ctaagattgg
     2701 tggtatgaat tgtgctcaaa aaccacattt ccagcaaaaa catgccacta gaataggtgg
     2761 tttttgacat gatagctata gttgactatg gagcaggcaa ccttaggtca gtgactaatg
     2821 cgcttgattt tctcaaagca aaatataatg ttacagataa gccaaaagac ctgatagcag
     2881 ctgataagat aatctttcca ggggtaggaa gctttggtga ctgcatcgct tcgttaaaag
     2941 ataaggggct gtttgatact ttaaagaaag agacaaaaaa caagccttat ttagggcttt
     3001 gtcttggact tcaggtgtta tttgagggtt cagaagagtc aaaaggagta aaagggctgt
     3061 ctatcttcaa aggccagtgc aagaggttca ctaagcttaa ggttccccag ataggctgga
     3121 attcgatcag tctatcaaaa aaagaccaca tacttagtgg aatagaagaa aattcatatt
     3181 tctattttgt gcattcgtat tacgctagac ctcaggataa aagcatcatc cttacagaga
     3241 cagattatgg cataaagtat tgcagctcaa tatcgcaagg caacatcttt gcattccagt
     3301 ttcatcctga aagatccgga gaggttggat tgagcattat aaacaacttc atcaaactat
     3361 gttaaaagcc attttattcg acatggacgg agtacttata gattcacatg atgcctggct
     3421 ggagagattc aacgcttcaa ggaggcattt tggattcaag gacatctcag ctgaggattt
     3481 tgacagagac gtctgggcaa ggaattttgt agagacagtt gcacaatatt tccccggaag
     3541 gacagtagaa gatgtaaggg gtttttactt taatactttc aggagtttca agataaaaag
     3601 gatgcctggt gcagacgaag ctctaatgta tataaaaagt aagggcctaa agacagcagt
     3661 tgtatcaaac acccagactc ctttggtaga tattatactt gaggatatcg gtataaggaa
     3721 gtactttgat attatagtcg gcggagagag ggtaaagaat gcaaagccca atccagagat
     3781 tgtgatgata gcctgcaagg aactggatgt tgattgctca gaaacgatat ttattggcga
     3841 tactatctac gacaaacaag cagctcaaaa agcaggctgt aagtttgtcg gctttaggta
     3901 tgatagcggg ataaaaaatt tggttgactt aagagggatg atataatgct cgcaaaaagg
     3961 attatcgcat gcatggatgt cgatgcagga agggttgtaa aagggataaa ttttgtagga
     4021 atcaaagacg caggaaaccc tatagcccta gcaaaagaat acaacaagca gggtatagat
     4081 gaactagttt tcttggacat aaccgcttcg catgaaaaga gagatatcat ggctgaactg
     4141 gtaaaaaaag ttgcagaaca gatatttatc ccatttacag tcggcggcgg attgagaagt
     4201 attgaagata taagaaagat actcaatgca ggcgcagata aagtttccct taacacagcc
     4261 gctgtcctaa atccaggtct tatcaaagaa gcatccaata aatttggcag acaatgcatc
     4321 gtgcttgcaa tagatgcaaa aaagaacatt aagatgaaga atggatttga ggtatatacc
     4381 tatggcggaa gaaaaagtac agggattgat gctgttgaat gggcaaaaaa aggagaaaaa
     4441 cttggcgctg gtgaaatctt gctcacttcc atggacaggg atgggacaaa gcagggattt
     4501 gacctggcgc ttacttcaaa gataagcggc agtgtaggaa tacctgttat agcatcaggc
     4561 ggtgtcggta atattgagca cttctacgat gcatttacaa aaggcaaggc agatgcagcg
     4621 cttgcagcat ccatattcca ctataaagaa tactcggtta aggagataaa agattatctg
     4681 gcaaaaaaag gtatcattgt aagacgatga agattcttgt actggactat gacggagtta
     4741 ttgtggactc tgagccagag gcatttgctg cctctatgaa ttgctatgcg aaattcaaag
     4801 gaaaagatcg gctgtttaca gtttgggaca taaaagatat cgatttaaag tacaagacat
     4861 tgcatgagaa aaataaggcc ttaaggccct atgtttcaac agtagatcat atatatgcta
     4921 taatggaagt tattgagaga ggaatcaagg tagagaatga tgcccaattt tataactttg
     4981 ctgcaaatat aaatgtccct tctggtttca aagaagagtt tcatcgtgag agaaagcgat
     5041 tccaggaaga agactttgat gagtggatga agtctacaaa gccttttgcg caagcattag
     5101 ctggactaat agccgccgta aaaagatttg ataaggtggt gatagtcaca gcaaacaaaa
     5161 atgaattgat ctcaccggta ttggagaaga ttggattaag ctatgatgag ataatcgaca
     5221 cttattattc aaaggataag tatggagcag ttgactccat tgctgagaca tacgatgctg
     5281 actataagga catttatttt gtagaagata gtgtgttctg gatggagaag atgataaaaa
     5341 aaggagtccg atgcttccta gcaacttggg gctataacac agaagcacag agaaaagagg
     5401 ctgatgggat aggtgcaaca ctgcttgatg aaaaaggcat tgttaaggag ttaagatgaa
     5461 acctaatgaa ggaaggctgc agactggaga acatatcctg gcaagggttg tgcagaacag
     5521 ggtgcctgat gcaaaggtag ccatatcaag gtttgacaag gaggatgaag gctctgtaga
     5581 tatcatttca agcacggatc taaggcagct tcaaaacctg gagccagcag taaatgccat
     5641 cataaaggca ggcttcaggg tggatgctcg ggaatttgca agggaagagt tgacagaatt
     5701 tgacctgagc agagtgcctg cagagcttaa caagataagg atcgtcgaga ttgtggggtt
     5761 tgataagagg ccatgcaagg acccgcatgt ggataataca gccaaagtag aaagattcat
     5821 cctcaaaaaa attgaaaggg caggcaagga caggtataag ttcacattta ctgtaactgc
     5881 tggatagtaa cgtaaacgaa agatatttaa aggaagcctt ttttattttt gctattatgt
     5941 ggtttaacac aaattttttt gtagccccta aaaaatttaa catgaacggc caggaagaca
     6001 taactaaaga gacctctttt tcaatttctg gagaaaaaaa gaaaagttag taagatatcg
     6061 gtttttctct aagactgaac tcagcaagaa catcgcggcc ttccaatatt ggcatgccct
     6121 tgtcaaatac aaagatggca gattggatgt ctccattagg atgcataagt ccttcagcac
     6181 gcttatatct tccctgcatg gaggcatagc cctctaagct agatatgagc ggccatatat
     6241 cctgcaaagc ttctcttgaa ttttgcccgt attcttcatc tactcgaaag ttcatcgcaa
     6301 gtttcccata tccaattttg cctaactgcc tcaaacgctt gtcatgctgg atctcatatc
     6361 taatatcaac aatcagttcg tcttctctaa caacacaagc gatagactgg tatcttttga
     6421 agtcattctc aggagctggc ttcatatcac gcgtttcaag tgctattacc tcaagtgcac
     6481 cctcgctaaa atgctcgatg agtgcatgtt cgataaatcg tgcattatcc cgtacctcct
     6541 ttggcacgct ctgatctttt gcaaaagatg gaagaagcct tccatacact ctcaagatag
     6601 tttcagcatg gggtttgtca aatagctcaa gataatgttt tatttgggaa taaggcatct
     6661 gttgcataac ccagattata acttatagga ttataaaccc attaccacaa tatttaaaaa
     6721 aagacttagt tatcgtttgt catatgatat ttccaagcat cgatttgatg gacggcaagg
     6781 ttgtccagct ggtccaaggc aagaaagaaa ataaaaagct tgagatagat gatgtaattg
     6841 gagttgccaa gaagtttttg cagtatgaaa tctcagtcat agacttggac gctgcaatgg
     6901 gaaatggtga taatcttgct ataatcaaac aattgtgcgc aaaatttaga tgccgtgtcg
     6961 gcggcggcat aaggacagtt gaaaaggcaa aagagcttat agattcaggt gcaaaaaagg
     7021 tgatcatcgg ctcctcagca ttcacaaaac aaggcataaa tttaccattc ctaaaaaaat
     7081 tggttaaaga gataggcagg gaaatgataa taatcgccat agacagcctt aaaggaaaga
     7141 tagtgataaa aggctggaaa gaatcgacag ggatagatac cctaagcgtt gtaaaagaac
     7201 ttgagccata ttgttcagaa tttctataca cctatgtaga caaagaagga atgatgcaag
     7261 ggactgatct ttctacgcta aaaaagctta aagccattac aaaggacgaa atcacagcag
     7321 ctggcggtat ttcttcgatg gatgagatag atgagctgca aaagattgga gttaactctg
     7381 ttctgggcat ggcgctttat accgggaaaa tcaggctgcc gtaaattgtt gaatctgtaa
     7441 acaaaagatt taatcatttt acatctggca taaatatctc tcttttattt tttatatcat
     7501 caatgaatag tatctacaga accataagaa acgccctgct ggcaagcagc cttgcattag
     7561 gaacatacac tatgcctgca aaagcagatt cccaagcgaa actgaacaac aataccctag
     7621 aaactgcggt tgtagttaac ggtgggaaaa gcccagtaag aaaccgcctg ctgactgatt
     7681 ttctcttttg aaaatgatga tttagaattt agattcagcg gcttaaacga gataaatgat
     7741 tgtgattcca atacttattt tggaggcaat aggctttttg ttggaagaaa agattatccg
     7801 ttaaaatttc ttgtggacac tatctcaagc aaaaatatgt ttgagggtaa gttaggtgtt
     7861 agccacacag atttgatgag gagattaggc ggttatggcg acgttagtgc ttatgcaaat
     7921 gacaaggcag caaacctaag ctttttttat ggaagaaaat taggtgatgg tctttcaata
     7981 gggctgtttc agctcatgga gtttccattt gaagataagc ccaaatacta taccgaattg
     8041 cagctgaaca agcagttaga taaacggcta tctctttttg gcagaataga gcttacagga
     8101 ttcaaggatc ctgtctatct ttttgggatg tctataaatc tggcagatgg atagcgctga
     8161 attgtggctt ttatcattct gataagtaag cgcataacct gcaactagca catttatatg
     8221 tttaaaagat ttaaggagtg atatgaatcc tgtagtccat ttcgaatttc cagcagatga
     8281 taggaagcgc atgatagatt tctacgaaaa agcctttggt tggagaacca agcagcttgg
     8341 cgaagacatg ggcaactatg ttctggtcga aacgacagag accgaaaatc ggatggtaaa
     8401 aaagcccggt gcgataaacg gcggcctcta tgaccggaag atgggaggaa atgcctcaaa
     8461 atgtccctca gtggttatct ctgtggacga tattaataag cacctggata tagttaaaga
     8521 agcaggtggt aaaattatag acaaacctat ggagatacca ggcgtaggct ggcttgtttc
     8581 ctttatcgat acagaaggca atgttctcag tatgatgcag ccggtggcga tgtgatgcaa
     8641 aagatagtcc ctaacctttg gtttgatgac aaggcagagg aagctgtcgc tttctatact
     8701 actactttca agaattctaa gatattaaag gtgactaaat atgggcaaga agggtctaag
     8761 atctccggta agaaggaagg ctctgtgatg acagtcaact ttcttatcga aacccaagag
     8821 tttgtggcgc taaacggcgg gcctgaattc aagtttaacg aagctgtctc ttttatcgtc
     8881 aactgcacag atcaggagga gatagactat ttctgggaca aatttggcaa agaaggagag
     8941 gaaggggtat gcggatggat aaaggacaaa ttcagccttt ggtggcagat tgtccctgaa
     9001 gggcttgatg acatgctctt gtctgagaaa gctatgaaat tatttcttgg catgaagaag
     9061 atcgagatag aggagttaaa aagggcaaga tgaataaaac cctgatgcca ataaatccaa
     9121 catattaagt tgaagatgat acctggcaaa
//
LOCUS       DASRUK010000079         6775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_397515, whole genome shotgun sequence.
ACCESSION   DASRUK010000079 DASRUK010000000
VERSION     DASRUK010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 6775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6775
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397515"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..394)
                     /locus_tag="VFF28_03490"
     CDS             complement(110..394)
                     /locus_tag="VFF28_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HZX44725.1"
                     /translation="MDKQMSRRDLGKVAAALALGMGVGGCEDEPVNSIIPGKFELKIH
                     SFLSGKKATEVDIGQDSLDITLGYEMQDVYLVDDPKGYLRIQVIPHKQAV"
     gene            complement(475..867)
                     /locus_tag="VFF28_03495"
     CDS             complement(475..867)
                     /locus_tag="VFF28_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44726.1"
                     /translation="MDSQMANAQKVAIDAKFILPGAENFSAYLEQRVGAGLTVTGPEM
                     DDGRQGAVKRWIVLYHGEIMGEYLQFNPGLEYRGRIPGYNNDLIRIANLVRKEELPED
                     IVCLSYGPSHDKADEPWQRLDGHDIPFP"
     gene            987..1757
                     /locus_tag="VFF28_03500"
     CDS             987..1757
                     /locus_tag="VFF28_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44727.1"
                     /translation="MALEVSQALEGMFQAIMDLRSDALSLGPKASVVFEKAVEEFCRE
                     ERLPVTIMSLLSGKIAIATRPDYLMVIDPLNAPAPLEREVESLGVSMALVPCRHDGMI
                     VPSESRYGLLGNLVSGSYCVGGLGHGVVYHGVLSRFRKQIAEGSKAISLAQARIYVDS
                     PDGTDNIRGSPILGLIDIVHGAADAYICHSAAADCILGACPLIQEVGGRITDPDGELL
                     PPSPDLKKKYSFVAAGNQALHKQVVEFLKGNYKHATQL"
     gene            1802..2527
                     /locus_tag="VFF28_03505"
     CDS             1802..2527
                     /locus_tag="VFF28_03505"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF012677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HZX44728.1"
                     /translation="MLENLKRQDSVVYDLIQKEIARQKSSLCMIPSENHASVAVLQAM
                     GTPLSNKYAEGYPGKRYYSGNQFIDQIENIAIERAKRLFKAEHANVQPNAGSTANQAV
                     YFGMLNVGDKVMGMDLTMGGHLTHGSAVNFSGKLYKMVHYGVEKDTETIDYDRLAQLA
                     HKEKPKMIISGATAYPREIDFEKFTKIAHEIGAIHMADVSHIVGLILAGVHKNCQSAD
                     IITTTTHKTLRGPRSAIILCQNH"
     gene            2512..3462
                     /locus_tag="VFF28_03510"
     CDS             2512..3462
                     /locus_tag="VFF28_03510"
                     /inference="COORDINATES: protein motif:HMM:NF013931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44729.1"
                     /translation="MPESLTLTFDKLRDVRYGENPHQKGAIYKDPFPKETSVINSKVP
                     MEGKELSFNNYLDGDAAFELVKDFKEPTVAVIKHCNPCGIASRETIEKAFEDAYDVDP
                     MSAFGCVIAMNRRCSLKIAEMTKGKYIEMLIAPEYEKEALNYIKENKQNIRLLETGKI
                     TKSNEGITLKRVTDGILVETREWPDIYKLELKVVTRRKPTKEELEDLRFAWIVNKHTK
                     SNSVVFCKNRTAYGLGVGQMSRVDASIIATRKSAGRAEGGVMSSDTFFPFSNAVDEAA
                     KAGIKAIIHPGGSIRDKEVIEAADEHGMAMVFSGVRLFKH"
     gene            complement(3806..4411)
                     /locus_tag="VFF28_03515"
     CDS             complement(3806..4411)
                     /locus_tag="VFF28_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44730.1"
                     /translation="MAETISDGGNILGNLDLIVPGTWQPARDVIRDWKEGAKGIGVNT
                     AEEGLYEQSGGKYFFYQVPDVLFTDPMFRQRLFDGIVGNEFFYLPPDMREAVTLRRDM
                     RIPYDEVKLHFQHNSRHGYVLAQEQNSPTEARYLQQAFGEHVRPGFRAYLLSPDDLRL
                     DFEGHEQGLVARASSISPYGYFFPVDTHMIHDKGAIRGERR"
     gene            complement(4459..5226)
                     /locus_tag="VFF28_03520"
     CDS             complement(4459..5226)
                     /locus_tag="VFF28_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44731.1"
                     /translation="MLGKPKKSPHFDFFIDEQIYKSFYLLSVRISKRINNYRTACIGP
                     GFYPRKKVRQFIREHAKSFTSLGESYAYEEGLRRPFHLGKEGEVKDELFAPGIRPSLK
                     GLTYLAATLPLNIISVPFYQAAYGLGYIVARTIRDPQNKYTGIYHLTLKALRDPRYQD
                     VYGQFLLAQELPQPLNLEVEYLMEKSRRLPQGFATCAERSFGAARKLLRSARSNNLKK
                     MHYFGSQAHMTEIAYFLVHPDYSFGSLERYMAKAAAN"
     gene            5211..6740
                     /locus_tag="VFF28_03525"
     CDS             5211..6740
                     /locus_tag="VFF28_03525"
                     /inference="COORDINATES: protein motif:HMM:NF033551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HZX44732.1"
                     /translation="MAYLASFRNQNWLLPPNIKDMIPKDHICFLVEEVVDKLDFTSFD
                     IKYSGAGHPAYHPRILCKILTQAMIDRIRPSRAIARNSHENVVFMYLAENLVPDFRTI
                     SDFRKDNRDLLKDIFKITISTAKELGAIGLEQLSIDGSKLKACASNNSSITKEQLDII
                     EQYVNNELREGIEIDRIEDKHFKDCRGYDQLKDIDNKKVRSLIAKYYKQIKNGGHDRV
                     SEIKNTLKEARDELEENKLEKISLSDPDSRFMVNKKGRKELSYNPQIAVDHKNGFIVA
                     NDICQEAVDNHQLEPQIKLVEENCGALKDETKITLDNGYYSGENIDYLNKRRLDGYIP
                     DQQEAKKAKGKEITLGRFDKANFRYDHKADEYMCPENKRLIFSYETYDKGKKKIVRSY
                     RGIACIGCPFGGECTKSRSMIRKLKVYRFEKERRDMAEKMKTKEAKEIYRERKEVVER
                     VIGHYKENLGFRSFLTRGLKAVKNEFNLACAAHNIRKIWILKRQNLSIARQIPPVLAF
                     D"
BASE COUNT         2155 a         1406 c         1465 g         1749 t
ORIGIN      
        1 cgctgcccat aatataagga aaatatggat cctgaaaagg cagaatctta gcatcgctag
       61 gcaaatccct ccagtattag cttttgatta gctagcgctt attctattgt cagacagcct
      121 gtttatgtgg gatcacttgt atccttaggt aacctttggg atcatccacc agatacacat
      181 cctgcatttc atagcccagt gtaatatcaa gtgaatcctg tccgatgtca acttctgtcg
      241 ctttttttcc tgatagaaaa gagtggatct ttagctcaaa cttaccagga atgattgaat
      301 taactggttc gtcctcacag ccaccgactc ccatcccaag agcaagtgca gcagcaactt
      361 ttcccaaatc tcttcttgac atttgtttat ccataagaaa acacacccca atcgttcaat
      421 ggattaatga cggccttata aatctaagct tgatacctta gcgatgaata tactctaagg
      481 aaacggaata tcatggccgt caagcctttg ccatggttca tctgctttat catgcgaagg
      541 accataactc agacatacaa tgtcttcagg tagttcttcc tttcttacta agtttgcaat
      601 tcgtattaaa tcattgttat atcccggaat tcgcccccta tattcaagac ctggattaaa
      661 ttgaagatat tcacccataa tctcaccatg gtataatact atccatcgtt taacagcgcc
      721 ttgccttcca tcatccattt caggtcctgt tacagttagg cctgctccga ctctttgttc
      781 cagataggca gagaaatttt ctgcaccagg caaaatgaat tttgcatcaa tagcaacttt
      841 ctgagcattt gccatttggc tatccataag aaaaacaccc cctaattcaa cacctgtatg
      901 tttacagcct tataaaccta agctgaccac acaagccctc aaagcctcag ctaagattaa
      961 ttaatcatga ttgcttaagg taaagtatgg ctcttgaggt tagtcaagct ttagaaggca
     1021 tgtttcaggc tatcatggac ctaaggtctg atgcgcttag cttgggacca aaagcatcag
     1081 tagtatttga gaaagcggtt gaagagtttt gcagggagga gcgccttcct gttaccataa
     1141 tgagtcttct atctggaaag atagcaattg cgacaagacc tgactacctg atggtcatag
     1201 atcccctaaa cgctcctgcc ccgctggaga gagaagttga atcattaggt gtcagcatgg
     1261 cattggttcc ctgtaggcat gatggtatga ttgttccatc tgaatcaaga tatgggctcc
     1321 tgggaaacct tgtctcaggc agttactgtg tgggaggcct tgggcatgga gtggtttatc
     1381 acggagtgct cagcaggttt agaaaacaga tagcagaagg ctcaaaagcc ataagtttgg
     1441 cgcaagctcg tatatatgtt gattctccag atggcactga caatataaga ggctcgccca
     1501 tactgggctt gatagatatt gtgcatggag cagccgacgc atacatatgc cattcagcag
     1561 cagcagactg catacttgga gcctgccccc tgatacagga agttggtgga aggataactg
     1621 atcctgatgg agagttgctg ccgccaagcc ctgacttaaa gaagaagtat tcctttgttg
     1681 cagcaggcaa ccaggcgctt cataagcagg tggtagagtt tcttaaaggt aattataagc
     1741 atgctaccca actatagaaa tctatttaaa ccaagccaaa aaagtaacct tcggtggtat
     1801 tatgcttgaa aacctaaaga gacaagatag tgttgtttat gaccttatcc aaaaggagat
     1861 agcccgtcaa aagagcagcc tttgcatgat cccttctgag aaccatgcat ctgttgcagt
     1921 gcttcaggcc atgggaaccc ctctctccaa taaatatgca gagggctatc caggaaaaag
     1981 atactacagc ggcaaccagt ttatcgacca gatagaaaat attgcgatag aaagggcaaa
     2041 aaggctgttc aaagcagagc atgcaaacgt ccagccaaac gcaggctcga ccgcaaacca
     2101 ggcagtgtat tttggcatgc ttaatgtcgg cgacaaggtc atgggcatgg acctgacgat
     2161 gggcggccat ctaactcacg gctcagcagt caacttctca ggaaagctct ataagatggt
     2221 ccactacggc gtcgaaaaag acacagagac catagactac gacagattag cccagcttgc
     2281 acacaaagaa aagcccaaga tgataatctc aggagcaaca gcatatccaa gagagataga
     2341 ctttgaaaag tttacaaaaa tagctcatga gataggcgct atccacatgg cagatgtcag
     2401 ccatatcgta ggacttatcc ttgcaggagt ccacaaaaac tgccagagcg cagatataat
     2461 cacaactaca acccataaga ctctaagagg tccaagaagc gccataatcc tctgccagaa
     2521 tcattaacat taaccttcga taagctaaga gatgtaagat atggagaaaa ccctcaccaa
     2581 aaaggagcta tatacaaaga ccccttccca aaagagacct ctgtgataaa ctcaaaagtg
     2641 ccgatggaag gaaaagagct gtctttcaat aactatctag atggtgatgc agcatttgag
     2701 cttgtcaaag atttcaagga gccgactgtt gcagttatca agcactgtaa tccatgcggt
     2761 atagcttcaa gagagaccat tgaaaaagca tttgaggatg cctatgatgt cgaccctatg
     2821 tcagcctttg gctgcgtcat cgctatgaac cggagatgca gcttgaagat tgcagagatg
     2881 accaaaggaa agtacatcga gatgttgata gcacctgagt atgaaaaaga ggcgttgaat
     2941 tatataaaag aaaacaagca aaacatcagg cttctggaaa caggtaagat aacaaaatca
     3001 aacgaaggca ttacattgaa gagagtgaca gacggcatcc tcgtagagac aagggaatgg
     3061 ccagatattt acaagttaga actcaaagta gtcacaagaa gaaagcctac aaaagaagag
     3121 cttgaagacc taaggtttgc atggatagta aacaagcaca caaagtccaa ctcggttgtt
     3181 ttctgcaaga ataggacagc atacggcctg ggtgtcggcc agatgtcgag ggttgatgca
     3241 tccattatcg caacaagaaa atccgcagga cgagcagaag gaggagtgat gtcctctgac
     3301 acttttttcc catttagcaa tgcagtcgac gaggcagcaa aagcaggcat caaagccata
     3361 atccatcctg gcggctcgat aagagacaaa gaagtgatcg aagcagcaga cgagcacggc
     3421 atggcgatgg tattttcagg agtacgttta ttcaagcatt gactagaatg caactttggc
     3481 atccagtgca ataaacgctt gaattttgca atagcaaaat tcctactgcg ttttcccaag
     3541 tttttttgca gtagcaaaaa aagctgatgg cgatggtatt ttatggtgtg cgtttgttca
     3601 aacactgatc agctaatgca actttagcat tgactgttat taacagtgaa tttgcctaag
     3661 caaattccaa ccagttttcc caagtttttt tgcagtagca aaaaaagctg atggctatgg
     3721 tgtttagcgg agtgcgtttg tttaaacact gatttttttt agttattcag gctttaagta
     3781 ataagggtat gtttctttgt gttagctatc ttctctcacc ccttatcgcc cccttgtcgt
     3841 gtatcatatg ggtatccaca ggaaaaaaat acccgtaagg gcttatgctt gatgcacggg
     3901 cgaccagacc ctgctcatgg ccttcgaaat caagcctaag atcatcaggc gataaaagat
     3961 aagccctaaa tcccggcctt acatgctccc caaaagcctg ctgaaggtat ctagcctcag
     4021 taggagaatt ttgctcttgc gcaagaacat agccatggcg gctattgtgc tgaaaatgaa
     4081 gtttgacctc atcatagggg atcctcatat ctcgccgaag tgtgacagcc tctctcatgt
     4141 caggtggcag atagaagaac tcattgccga caatgccatc aaacaacctt tgcctgaaca
     4201 tgggatctgt aaacaagaca tcagggacct gataaaaaaa atattttcct ccagattgct
     4261 catagagtcc ttcctcagcg gtatttaccc ctataccttt ggctccttcc ttccagtctc
     4321 ttatcacatc cctggcaggc tgccaggttc caggcactat cagatcaaga ttacctagaa
     4381 tatttccacc atcggatatt gtttctgcca tgttaatgac ctcctttaga atagcattaa
     4441 tcaaaaaaaa tatttaaatc agttggctgc agccttagcc atataccttt ccaaacttcc
     4501 aaaactgtag tctggatgca caagaaaata tgctatctcg gtcatatggg cttgacttcc
     4561 aaagtaatgc atttttttga ggttgtttga gcgtgcagac ctcaaaagct ttcttgctgc
     4621 tccaaaggat ctttcagcgc aggtagcaaa accctggggc agcctcctgg atttctccat
     4681 tagatattcc acttcaagat ttaatggctg aggtagctct tgagcaagca agaactgacc
     4741 gtatacatcc tggtatcttg gatccctcaa tgcctttaat gtcaagtgat agatgcctgt
     4801 gtatttgttt tgtgggtctc tgatagtcct agcgacgata taacctagcc cgtacgcagc
     4861 ttgataaaaa gggacagaga tgatgttaag gggcagggtt gcggctaggt atgtaaggcc
     4921 tttcagtgat ggccttatac ctggtgcaaa caattcatct ttgacctctc cctcttttcc
     4981 tagatgaaac ggtctgcgta aaccttcctc ataggcatag ctttccccca gactagtgaa
     5041 tgattttgca tgttctctta taaactgcct tacctttttt cttggataaa accctggccc
     5101 tatacaggct gtccgataat tattaatcct cttgcttatt cttaccgaca gcaaataaaa
     5161 agatttatat atttgctcgt cgataaaaaa gtcaaaatga ggtgatttct ttggcttacc
     5221 tagcatcgtt tagaaaccaa aactggcttt tacccccaaa catcaaagat atgatcccca
     5281 aagaccatat ctgttttttg gtggaagaag tagtagacaa gttggatttc accagttttg
     5341 acatcaaata ttctggggca gggcatcctg cataccaccc aaggatactc tgcaaaatcc
     5401 tcacccaggc aatgattgac aggatcaggc catcaagagc aatcgcaaga aacagccatg
     5461 aaaatgtcgt ctttatgtat cttgctgaaa atttggttcc tgatttcagg acaatcagtg
     5521 attttaggaa agataacagg gatttgctta aggacatctt caagataaca atcagcactg
     5581 ctaaggaatt gggggcgata ggtcttgagc agttgagcat agatggttca aaattaaaag
     5641 catgcgcctc caataacagc agtattacaa aagagcagct tgacattatt gagcagtatg
     5701 tcaataatga gctaagggaa ggtattgaga tagacaggat tgaagacaaa catttcaaag
     5761 attgcagggg ctatgaccaa cttaaggata tagataacaa gaaggttagg tcattaatcg
     5821 caaagtatta caagcaaatt aagaatggcg ggcatgatag ggtttcagag ataaaaaata
     5881 ccctcaaaga agcaagagac gaacttgagg agaataagct cgagaagatc agcttaagtg
     5941 atcctgactc aagatttatg gttaataaga aaggaagaaa agagctgtcc tacaatcctc
     6001 aaatagctgt tgaccataaa aatggattta tcgtggcgaa tgacatttgc caagaggcag
     6061 tagacaatca tcaactcgag ccacagatca aacttgtaga ggaaaattgc ggagcactaa
     6121 aagatgaaac aaagattact ttagacaacg gatattatag tggagaaaat atagattatc
     6181 ttaataagag aaggctggac ggatacatac ctgaccaaca ggaggcaaaa aaagcaaaag
     6241 gcaaagaaat caccctcgga agatttgata aggccaattt cagatatgac cacaaggcag
     6301 atgagtatat gtgccctgaa aacaagaggc taatattcag ctatgaaacc tacgataagg
     6361 gaaagaaaaa aatagtgagg tcctatcgag gtatagcttg cattggctgt ccatttggtg
     6421 gagagtgcac aaagagcaga agcatgataa gaaaactaaa agtatatcgt tttgaaaaag
     6481 aaagaagaga tatggccgag aaaatgaaga caaaagaagc aaaggaaata tatagagaaa
     6541 gaaaagaagt ggtagaaaga gtgatagggc attacaagga aaatctggga tttagatcat
     6601 ttctgacaag agggttgaag gctgtgaaga atgagtttaa cttagcatgc gctgcccata
     6661 atataaggaa aatatggatc ctgaaaaggc agaatcttag catcgctagg caaatccctc
     6721 cagtattagc ttttgattag ctagcgctta ttctattgtc agacagcctg tttat
//
LOCUS       DASRUK010000080        12925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2073039, whole genome shotgun sequence.
ACCESSION   DASRUK010000080 DASRUK010000000
VERSION     DASRUK010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 12925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12925
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2073039"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1318
                     /locus_tag="VFF28_03530"
     CDS             116..1318
                     /locus_tag="VFF28_03530"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HZX44733.1"
                     /translation="MKYIIFVIDGAGDYPVEELGDKTPLMAARKPNIDSIAKKGRCGL
                     FRTIPDNFDTCSTVANLSIFGYDPLKYFQGRGVFEAAAMGIKLADEDVAFRCNTICVK
                     NNSIKNHSAGHISNRESRILIQRLDKQLGSDRIKFYPGVSYRHLLVLRGYSKKVICSP
                     PHDHQGEDIRGLLPKAKEKEAEETTLVLRRLMTDSAAILQNHMVNINRVKEGKDPANM
                     IWPWSPGRKPKMKTYQELYGIKGAVISAVDLIKGIGIYAGFDVIKVEGATGLHDTNYE
                     GKADACIQALNDHDIVYVHVEGIDEAGHEGNAKLKVKCIEDFDKRLLGRVLENVELSQ
                     CSIAILPDHLTPVSIKSHIPSPVPFAIYKPGANPDSVSFFDEESCKKGSYGQLTGANF
                     ISNLLMKE"
     gene            complement(1345..2304)
                     /locus_tag="VFF28_03535"
     CDS             complement(1345..2304)
                     /locus_tag="VFF28_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44734.1"
                     /translation="MSTLNTVIPYEAFSRHVKMRPATLIRFIDQGLVLGRFEPLEVEI
                     QATTKWEKQAQQRGFEDPSQLYYISDLVQALGDGVWLKRFIEGMCVDALVSDSYKFLK
                     VNSGANNKSIVALDEQALFRYTGKGQGITEVLIAKEAAYELMHLLIKYGKHISDENIP
                     HVHNLSFKTPPTGTVELFRQSRPLLLYDGKSGSQLIYCSLGQTEGSRIAANNLFGKSP
                     DSAAVALDPGKLIDYAPDDGEIYTVALVDVVDPKIFFSHPLAGKEYQVRQFRQLDRQA
                     TALRQSLNGQKMIDGPNQNLDLIVNAYNTNAAIRYGSLRKQPA"
     gene            2445..2726
                     /gene="albA"
                     /locus_tag="VFF28_03540"
     CDS             2445..2726
                     /gene="albA"
                     /locus_tag="VFF28_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006325 - chromatin organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein Alba"
                     /protein_id="HZX44735.1"
                     /translation="MADDNSIFIGGKPFMNYVTGVVMQFTTKNAPEVIVKARGKFISR
                     AVDVAEVVSKRFLTGQINVRDIRINSEEFQNKEGRDVRVSTIEIALGKL"
     gene            2798..2885
                     /locus_tag="VFF28_03545"
     tRNA            join(2798..2835,2851..2885)
                     /locus_tag="VFF28_03545"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2832..2834,aa:Arg,seq:tcg)
     gene            complement(3038..3427)
                     /locus_tag="VFF28_03550"
     CDS             complement(3038..3427)
                     /locus_tag="VFF28_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44736.1"
                     /translation="MNYISYTYWQSVERKEYEAMVQLCEGKLSMVEYVKVYADFLRNE
                     EELCLFPKNKTEFVQIMGYQSFYTSPEKEWEHEMEHYSLILEEGLKPRIAIVFLKNDI
                     NDKPPITFRKTFTVDNRHEIIGQLRIF"
     gene            complement(3532..3753)
                     /locus_tag="VFF28_03555"
     CDS             complement(3532..3753)
                     /locus_tag="VFF28_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44737.1"
                     /translation="MKGLTGRLREKPKSRGGKILKPTKQEIKEMQEFAKRSATSIMDA
                     IGKKCLSNALACKDYSDENVQRYLEHFRS"
     gene            complement(4091..4543)
                     /locus_tag="VFF28_03560"
     CDS             complement(4091..4543)
                     /locus_tag="VFF28_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44738.1"
                     /translation="MNIEDLVKNQLALISIEENLHHLCYDLVIRAQEIYGVIKSVKEA
                     QGYLSELICAANYQPRNTSIFHPLQYYEDWRMKKTKDFVNELMDQQFTPFAQAVDGLA
                     DTYDKDVDLNSLYRKAEVLDKALLQLKDYQHRSQKIWQRLRPSLEPTG"
     gene            complement(4655..5137)
                     /locus_tag="VFF28_03565"
     CDS             complement(4655..5137)
                     /locus_tag="VFF28_03565"
                     /inference="COORDINATES: protein motif:HMM:NF025743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HZX44739.1"
                     /translation="MNNLLFAVMYSDKEIYDKSLLKLIAAYGAVKDECPEYDFDKFTP
                     YYEEEMGKGLTKRFVVFEKAIEKKDLVSIKKETTDLEKKFSVDGKRQVNIDPGYIGQY
                     ELVLASFKRGRGYKQELGDGVYAHKVLEFDDGGVQTFWHTFPDYKENKESLIGLAKRD
                     "
     gene            complement(5200..5766)
                     /locus_tag="VFF28_03570"
     CDS             complement(5200..5766)
                     /locus_tag="VFF28_03570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZX44740.1"
                     /translation="MGPDGKKYSPNRNGAFQYFMDACSNGRYNTYISIIASFGIFRES
                     ADEVMQQVAYDLCRQSLTEGQIQNLDVYVRKAIENQCKYSLRKNIRLRRLNQVATYAW
                     RQTDTRSPFASAVREEHHRLLVRAYKGLIAEHREVITLHYKQNLSISNMAKLLNIPEG
                     TVKSRLNRALSCLKQFFDRDEYNIYDFV"
     gene            5856..6641
                     /gene="proC"
                     /locus_tag="VFF28_03575"
     CDS             5856..6641
                     /gene="proC"
                     /locus_tag="VFF28_03575"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HZX44741.1"
                     /translation="MHKIGFIGAGNMAFALAKAIKAKKLSNSIIASDVSQDRLDYVKK
                     ELKIKTTLSNREVEEASDIIFLAVKPQVIDKVLEDIAHTEKLIISIAAGIKLSKLEAK
                     LKKARVIRVMPNTPCLVGEMAAGFAAGKKARDQDIKVVKEILSAAGKAYFLKEELLDA
                     VTGLSGSGPAFVARLIESFEEAGKKLGLGKDISRELSVQTFLGTAKLLSETAMSPEEL
                     VNMVSSPNGTTVAGREILESSDVREVMIKTISRAAERSKELGK"
     gene            6638..7948
                     /locus_tag="VFF28_03580"
                     /pseudo
     CDS             6638..7948
                     /locus_tag="VFF28_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084344.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
     gene            8053..8631
                     /locus_tag="VFF28_03585"
     CDS             8053..8631
                     /locus_tag="VFF28_03585"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HZX44742.1"
                     /translation="MIEILKCFITLFVIINPIGNLTIFIGLSKGMDNRRIFKAVNDIL
                     FVACILLFSFIFFGEYIFNFFGIGLDSFQIGGGIVLLIIAITYLLGIHRRTQHDNDIS
                     IPMGTPLMIGPGTITSALILVNQYGTLATFMGAAMALTAIWIVLRYSSTIYRLMGTHW
                     TMVASRIMGLILVAISVEFIKDGIAFFVKTNF"
     gene            8658..9704
                     /gene="proB"
                     /locus_tag="VFF28_03590"
     CDS             8658..9704
                     /gene="proB"
                     /locus_tag="VFF28_03590"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HZX44743.1"
                     /translation="MAYKRIVVKIGTNLLTDKLCGLKVEQIQDIARQISVLKSKGIEV
                     LIVTSGAIGAGCTELGVKKSREVETRQALAAVGQSTVMRAYHDAFSRYKAKVGQILLT
                     YEDFSNRTRYLHLKKAIERLLDFGVIPIINENDPISVDEIGATFGDNDKLSALVASKV
                     NADLLIIVSDIDGLYDKNPKLSKDAKLIRRVESITKDIENMAGKAGSAFSVGGMESKV
                     KAAKIVMDFGCDMIICNGKKKDILQKALEGKEGTLFVAANKLNGKQRWIKFAKEKGKI
                     RVNDRAKDILLDGKSSLLAVGVVGVEGEFDKGEVVLINDFAKGISDHSSKELANINGK
                     KGKVVIKRGNIVIL"
     gene            complement(9731..12685)
                     /locus_tag="VFF28_03595"
     CDS             complement(9731..12685)
                     /locus_tag="VFF28_03595"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HZX44744.1"
                     /translation="MVNIRADDRIGAPGGFYWIITTKKSVTVDLAKARSTRFYYGIKS
                     SDKTKWDINSFEINKATCFDRKQNQGETDIDCGSGCIKCGLGKSCISKSDCESNFCLN
                     TKCAPALCPGTDNSCGLSSCTDCNQKDSCEMDHFIDYSCRGGDCVSAKDDCKDCSCSC
                     GGYNLQESAANGNCKDGKDDDCDGSIDTDPECKKSSQCTACQESPLKVYPSYNIRAGE
                     EALFNAFSYLKSDPSLTRFDWDFGDTYSLKYPEGGVSVTHTYMYPGKYNVTITMTNLQ
                     GTITKAIVPISVEGIPARISTISLLNPIIRMNFDGSLNEASGILTSYQGSQKYTYGPV
                     YRGFDLGGSFVRIQNKPTTLNKFTISFFARQKDLTKGGVVLDAGSLALAYVSEWRLSL
                     DVKTDKGNCALLSNWETLSHNTDWNHYAIIYDGSKLKLYINGKEPVQAASCAGNVELG
                     NIVLGASKSGNNIFNAEIDEFEIVQEALDIEEVLYGFELEHANFHARTAQYITANIPD
                     ELISKSASRLVVKISSNKGYESIVYDKRGLSKKERFLLKNGELKAGSYTLGAQLLDSS
                     NNMIAEIKEYFDKPYDGIPQVGIDENNAIRVNGELFFPVTPWGLNDMDFPKWLGKDKA
                     INSLYGTAFWHGGGFGATKNLQSWKYYLDEAKKYNTLVLGPSGWDGLKGKVTSLDNLM
                     EYVNATKDSNILAWQWMDEPELGGYEAVSSQALRSWTLASHLGDPQHPVATNFAGPGW
                     IAEGDVWDRAHRRKYEYLYDASLFGGKKTPVADILGFDFYPIDWALPHQRGATVTQLA
                     EIMDKIRQENADLAPVFSWIETSDINEPPYSTQWPPTPEQVRMLAWLNVVHGAKGINW
                     FHYHLPSPNGNFVAMRKFVSQITHLAPIVLSGDSVITISDTANVPGNRVDTMVKRNGT
                     DIWLFAVRLTETSPKGGTGETKDIKTTFSIPALREGIADVYDEGRQLKVYDGKFTDNF
                     KPNDMHIYKIRS"
BASE COUNT         3930 a         2653 c         2587 g         3755 t
ORIGIN      
        1 aggaattgct tggaatagag ccaaaagttc ctgaaagtct aagtcatctg gataataaac
       61 aggaaattta tgtcacagta gaaaatgatt attcagactt aaaacattta ataaaatgaa
      121 atacataatc tttgtcatag atggagcagg agattaccct gtagaggaat taggtgataa
      181 aacacccttg atggcagcac gtaagccaaa tatagacagc attgcaaaaa aaggcagatg
      241 cggtcttttc aggaccattc cagataattt tgatacttgc tctactgtag ctaacctctc
      301 tattttcggc tacgacccat tgaaatactt tcaaggaagg ggtgtgtttg aagcagcagc
      361 catgggcata aagcttgccg atgaagatgt ggcatttagg tgcaatacta tctgtgtcaa
      421 gaacaactct ataaaaaacc actcggctgg ccatatcagc aaccgtgaat ccaggatact
      481 tatccagcgc ctcgataagc aattaggcag cgacaggata aaattctacc ctggagtaag
      541 ctaccgccat cttcttgtcc tcaggggata tagcaagaaa gtgatttgtt caccccctca
      601 tgatcatcaa ggtgaagaca tccgaggtct tctgcctaaa gcaaaagaga aggaagcaga
      661 ggaaacaact cttgtgctaa gaaggttgat gaccgattct gctgccattc tccaaaacca
      721 tatggtgaac atcaataggg taaaggaggg aaaagaccct gccaacatga tctggccttg
      781 gtcgccagga agaaaaccta agatgaagac ctatcaggag ctttatggca taaaaggagc
      841 agtcatctcg gcggttgacc tgataaaagg tataggcatc tacgctggat ttgatgttat
      901 caaggttgag ggagcaacag gtcttcatga cacaaactat gaagggaaag ccgatgcatg
      961 tatccaagct ttgaatgacc atgatattgt atatgttcat gtcgaaggaa tagatgaagc
     1021 aggccatgaa ggcaacgcaa aactcaaagt aaaatgcata gaggatttcg acaagaggct
     1081 tttaggaaga gtgcttgaga atgttgaatt aagtcagtgc tcaatagcta tccttcctga
     1141 tcatctgacg cccgtttcaa taaaatcgca tattccttct cctgttccgt ttgctatcta
     1201 taaacccgga gcaaacccgg atagtgtttc tttttttgat gaagaatcat gcaagaaagg
     1261 aagttatggg caattgacag gtgcaaactt tattagtaat cttctaatga aagaataggt
     1321 tcaaacccct accaatggag ggttttatgc aggctgcttt ctcaaagatc cataccttat
     1381 agcagcattt gtattatagg cattcactat taaatccagg ttttgattcg gtccatctat
     1441 cattttctga ccgtttaaag actgtcttaa tgcagtggct tgccggtcaa gttgcctaaa
     1501 ctgcctcact tgatattctt tgccagccaa tgggtggcta aaaaatattt taggatctac
     1561 tacgtctacc aaagcaacag tgtaaatctc cccatcatct ggagcataat cgataagttt
     1621 acctggatcc aaagcaactg ccgctgaatc tggggacttg ccaaacaaat tgttagcagc
     1681 aatccttgag ccttctgtct ggcccagact gcagtaaatc aattgactgc cagatttgcc
     1741 atcatatagt aaaagaggtc ttgactgtct aaacagttca accgtccctg tgggaggcgt
     1801 tttgaaagaa agattgtgaa catgtggaat attctcgtcc gagatatgtt ttccatattt
     1861 tatcaggaga tgcataagtt catatgcagc ttcttttgcg ataagcacct cagttatccc
     1921 ttggcccttt ccagtatacc taaaaagggc ttgttcatca agagcaacta tacttttatt
     1981 gtttgcccca gaattgactt tcaaaaactt ataactgtca gaaaccaacg cgtcaacaca
     2041 catgccttct ataaatcttt ttaaccatac cccgtcccct aaggcttgga caaggtcgct
     2101 gatataataa agctgagagg gatcctcaaa acctctctgt tgtgcctgct tttcccattt
     2161 tgtagttgct tggatctcca cttccagagg ttcaaacctt cctaacacta atccttggtc
     2221 aataaatctt atcagggtag cgggcctcat tttcacatgc cgtgaaaatg cctcataagg
     2281 aatcaccgtg tttaaagtgc tcataagggc ttggattcta ttcttcttta taaacattac
     2341 tatttagtgg taactaatct atcaacgata catttaaaaa atataaaata ttagctaatc
     2401 gctattggtt tttagtaagt acaaaaacta cggaggagga taatatggca gacgacaaca
     2461 gtatttttat cggtggaaag ccatttatga actatgtcac cggagtggtg atgcagttca
     2521 cgacgaaaaa cgcaccggaa gttatagtga aggcaagagg caaatttatt tctagagcag
     2581 tcgacgtagc agaagtcgtg tcaaagaggt tccttacagg ccagataaat gtaagagaca
     2641 taaggataaa ctcagaggag ttccaaaaca aggaaggccg tgacgtaagg gtatcgacta
     2701 tagagattgc tcttggaaaa ttataaattt tattttcata ttttttaaac taattctgct
     2761 aaattttata tactcaaagc aaaataacaa tagccacggg cctatagtct aatggatcag
     2821 gacaagcggc ttcggtctct aaaggatgac accgcttaat cggggttcga atccccgtag
     2881 gctcatcacc catttggtta cttagaagga taacatttgt ttatctttat gattccgtaa
     2941 taaggagtcc actattttag ttgttcttca atgaactcta ttatatctaa atctccatta
     3001 ctgggaacac ttactttcga gacacgcata agcatcttta aaaaatcctt aactgaccaa
     3061 taatttcatg tcgattatcg accgtaaatg tttttctgaa agttataggg ggtttgtcat
     3121 taatatcatt ctttaagaaa acaatagcta ttcttggttt caacccctcc tccaatatta
     3181 aagaataatg ttccatttca tgctcccatt ctttttcggg agaggtataa aaagattgat
     3241 aacccattat ttgaacaaat tcagttttat tttttggaaa caaacataat tcttcctcat
     3301 tccttaagaa atcagcataa accttaacat attcaaccat agacaattta ccttcacata
     3361 gctgtaccat tgcttcatat tcttttcgct caacagattg ccaataagta tatgaaatat
     3421 agttcatttt ttcttcacca tatctacaat atatggaaaa ctcaatccta caccgagaaa
     3481 aataaatccc aacccctgat aagtcgcagc cttcctcata agaaatcact cttaagacct
     3541 gaaatgctcc aaatacctct gcacattttc atctgagtaa tccttacaag ccagggcatt
     3601 gctcaggcac tttttcccga tagcatccat tattgaagtt gcagagcgct ttgcaaactc
     3661 ctgcatctct tttatctcct gctttgttgg cttaagtatc tttccacccc tgctctttgg
     3721 cttctctctt aatctgcctg tcaagccctt catcagacat tttgctgatt tgtttaacct
     3781 tctttaagtt gagctttgga tttttctcat acatagcatt acctcttgtc agcttgctta
     3841 aataatctct tgttgatttt attcttaatt gacttattct tagcagactt ccccgaagta
     3901 gagcataaat aatattaagg agtgtatcaa tagattaaga cgttgggagg taagtcccaa
     3961 cgcttgtcga taaccatgca aatagatatc gacaaaatta attatcgagc aaagcaagat
     4021 agcattgatc atccgctggc ttataatgct aattccggaa aacttgtttt gcatatccct
     4081 tatgagtgca tcaaccagtg ggttctagag aaggcctgag cctctgccaa attttttgac
     4141 ttcggtgttg ataatcctta agttgcagca gtgctttgtc caacacttct gccttgcgat
     4201 aaagagaatt taggtccaca tccttatcgt atgtatctgc aaggccatct acagcttgag
     4261 caaatggtgt aaactgttgg tccatcaact cattcacgaa gtctttagtt tttttcatcc
     4321 tccaatcttc gtagtattgc agaggatgaa agatagaagt attgcgcggt tggtaatttg
     4381 cggcacaaat tagttcactt aaatatcctt gtgcttcttt gactgatttg atgacaccat
     4441 aaatctcctg tgcccttata acaagatcat aacaaaggtg atgtagattt tcctcaatac
     4501 taatgagtgc taattgattt tttactaaat cctctatatt catgaggtat ttacagagat
     4561 atagcttatt tatttaaagt ttacccaaaa tacccctcaa aagtagatac ttattaccga
     4621 catacacagc tagcggaagc atataactgg atggctaatc cctcttggca aggccaatta
     4681 gagattcctt attctcctta tagtcaggaa atgtatgcca gaaagtttgt acaccaccgt
     4741 catcaaattc cagcacttta tgcgcataca caccgtcacc taactcttgc ttataccctc
     4801 ttcccctctt aaaggaggca aggacgagct catactggcc aatataccca ggatcgatat
     4861 tcacctgtct tttgccgtca acagaaaact ttttttccag atcagtagtc tcctttttga
     4921 tgctgaccaa atcttttttt tcgatcgctt tctcaaaaac cacaaatctc tttgtcaatc
     4981 cttttcccat ctcctcttca taataagggg taaatttatc aaaatcatac tcaggacact
     5041 catcctttac cgcaccatac gctgcaatca acttcaaaag agatttatca taaatttctt
     5101 tgtcagaata catgacagca aaaagcaaat tattcataag ctaaaaagaa ctatcagttt
     5161 ttaataaatc tttgcctgtg caagtaagtg aagataaagt tatacaaaat catatatatt
     5221 atattcatcc ctatcaaaaa actgcttaag gcatgacagc gccctgttta atctgctctt
     5281 caccgtacct tcaggtatat tgagcaattt tgccatatta ctgatgctaa ggttctgttt
     5341 gtaatgcagc gttataacct ccctatgttc ggctatcaag cctttataag ctcgcaccag
     5401 caatcgatgg tgctcttccc taacagcgga tgcaaaagga gaacgggtat ctgtttgacg
     5461 ccatgcatag gttgctacct gatttagtct ccttaatctt atattctttc ttagagaata
     5521 cttgcactgg ttttctatcg cctttcttac atacacatca aggttttgaa tctgcccttc
     5581 agtaagagat tgtctacaca gatcataggc tacctgctgc attacctcat cagcgctttc
     5641 acgaaatatg ccaaaagaag ctatgatgct tatgtaggta ttataccttc cattagagca
     5701 agcatccata aaatattgaa acgctccgtt acgattgggt gaatacttct ttccatcagg
     5761 gcccattatt gacaagaact gttttttatt tataaataat ttgagaaacc attacataaa
     5821 aagctttata aatatcattc ttcaaaatag gaatcatgca caaaatcggc tttatcggcg
     5881 ctggaaacat ggcttttgcc ctggcaaagg cgataaaggc aaaaaagctt tcaaattcga
     5941 ttatagcttc ggatgttagc caggatcgcc ttgactatgt gaaaaaagag ctgaagataa
     6001 agacaaccct cagcaataga gaggttgaag aggcttcaga tatcatcttt cttgcagtaa
     6061 agccccaggt tatcgacaaa gtgcttgagg atattgcaca tacggaaaaa cttattatct
     6121 ctatagctgc aggcataaag ctttcaaagc ttgaagcaaa gctaaagaaa gcaagggtaa
     6181 tcagggttat gccaaatacc ccttgtcttg taggtgagat ggcagcaggt tttgcagccg
     6241 gaaaaaaagc aagagatcag gacataaaag tcgttaaaga gatactcagc gcagcaggta
     6301 aagcttattt tctaaaagag gaattgctcg atgcagttac aggccttagc ggctcaggcc
     6361 ctgcttttgt agcaagactt attgagtcat ttgaagaagc aggtaaaaaa cttggcctag
     6421 gcaaggatat atcaagagag ctttcggttc aaaccttcct tggaacagca aaactacttt
     6481 ccgagaccgc aatgtctcca gaagagcttg taaatatggt cagctctcca aatggaacaa
     6541 cagttgcagg aagggaaatt ttggaaagtt cagatgtaag agaagtcatg ataaaaacaa
     6601 taagcagagc cgcagaaaga agtaaagagc ttggaaaatg agccttaagg aaaagatact
     6661 agagcaatgc gagaatgcaa gggaagcatc acttcaacta gcttccttat ccacagatac
     6721 aaagaacttt gttctatatg aactggcaga agatttgcta gcaaacagag cccagatact
     6781 aaaggcaaat cagaaagaca tcaaggacaa caaaaacagg atttcagagg taatgctaaa
     6841 aaggttaaaa gttgatgaaa ataaggtaaa agacatgtcc cagatggtcc taagcgtagc
     6901 aaagcttgaa gatcctgtgg gaaagctatt gcaaaagaca gagctggaca agggattggt
     6961 actggaaaaa gtatctgtcc ctattggcgt tatctgctgt atttttgaat caaggcctga
     7021 agtcgtagtc cagatatctg ctctatgtat caagtcagga aacgctgttc ttctaaaagg
     7081 gggctcagaa gcgataaaca caaataaagc gcttgccgat ataataagaa gatcgatcaa
     7141 agacaacaaa ggaatgcctg aagatgcagt ccaactgctt gaaaacaggg aagcagtaaa
     7201 agaaatattg aagatggata gttttgttga cctgataatc ccaagaggct caaacagttt
     7261 tgtaaaatac gtgcaggaca atacaaagat acctgttctt ggccatgcag aaggtatatg
     7321 tcatgtctat atcgataaag atgcagatat aaataaagcc atcgccatct cagtcgatgc
     7381 aaaatgccag tatcctgctg tgtgcaacgc catggaaaca ctgttgattc atagagcgat
     7441 agcaaaaaaa atccttcccc agcttgcatc tgcatataaa gaaaaggatg ttgagctaag
     7501 aggagatgca aatactgcaa agatcataaa aggcataaaa aaagccacag aagaggactg
     7561 gaagacagaa tacaatgatc ttgtgctatc gataaaggtt gtatcaaata ttgatgaagc
     7621 tatcactcac atcaataaat acggctcagg ccacacagat tctatcgtga cagaggataa
     7681 ggcatctggc cttaagttca tcaatctaat cgattcaagc tcagtgatgt ggaatgcatc
     7741 caccaggttt gccgacggct ttagatatgg tcttggagca gaggtaggca tttcaacaaa
     7801 caaagtccat gccagaggcc ctgtaggcct tgaaggacta gtaatctata aatatgtgtt
     7861 agttggaaat ggacatattg tcgctgacta tagtagtaaa aaaaggaagt ttacgcataa
     7921 gaagataaga tgtcagaaat tttccatcaa gcctgctgag ccaaagcgga gctttcagtc
     7981 aaggaagaaa atttctgagc atgctcaaaa accctcgaca gcacgctcct tattgcatgc
     8041 ggggtttttg acatgataga gatacttaag tgctttatca ctctctttgt aataatcaat
     8101 ccaatcggaa atctgactat ttttatcggc ctatcaaagg gcatggataa tcgtaggatc
     8161 tttaaagccg taaatgacat cctttttgtc gcctgtatct tgctattctc ttttatattt
     8221 tttggggaat acatcttcaa tttttttggt ataggcctgg acagcttcca gataggagga
     8281 gggattgtct tgttgataat agccataact tatctgctgg gtattcatag aaggacgcag
     8341 cacgataatg atatatctat tccaatggga acacctctta tgataggtcc tggaacgata
     8401 acttctgcat tgatccttgt aaatcaatat ggtacccttg caacctttat gggagcagcg
     8461 atggctctga cagccatttg gatagtactt aggtattcaa gcactatata tcgccttatg
     8521 ggaactcatt ggacaatggt cgcttcaagg ataatgggat tgatacttgt tgctatctca
     8581 gtagagttta tcaaggacgg catagcattc ttcgtcaaaa caaactttta aatagggatt
     8641 tagttctaaa gccacctatg gcttacaaac ggatagttgt aaagattggc actaacttgc
     8701 ttactgataa gctctgcggc cttaaagtgg agcagataca ggacatagca aggcagatat
     8761 cagtgctgaa aagtaaaggc atagaggtcc ttatagtcac atcaggagcc ataggtgcag
     8821 ggtgtacaga gcttggcgta aaaaaatcaa gagaagtgga gacaagacaa gcccttgcag
     8881 ccgtcggcca aagcacagtt atgagggctt atcatgacgc tttttccaga tataaggcaa
     8941 aagttggcca aatccttctc acctatgagg acttctcaaa taggacgaga tatcttcatc
     9001 taaagaaagc tatagaaagg ctgcttgact ttggtgttat ccctatcatc aacgaaaatg
     9061 accctatcag tgttgacgag ataggggcta cttttggaga taatgacaag ctaagcgcac
     9121 tagttgcaag caaggtaaat gctgatctgc ttatcatagt ctcagacata gacggccttt
     9181 atgacaagaa tcccaagctt agcaaggatg ccaaacttat cagaagggtg gagagcataa
     9241 caaaagatat tgaaaatatg gcaggaaaag caggcagtgc cttttcagta ggaggcatgg
     9301 agtccaaggt taaggcagca aagatagtca tggattttgg ctgcgacatg ataatctgca
     9361 atggtaaaaa gaaagatatc ctgcagaaag cacttgaagg aaaagaaggg actttatttg
     9421 tcgccgcaaa taaattgaac ggcaagcaaa gatggataaa gtttgcaaag gaaaaaggaa
     9481 agatacgggt aaacgaccgg gcaaaagaca tccttcttga tggaaagtca agcttgcttg
     9541 cagttggagt agtaggcgtt gaaggagagt ttgacaaagg agaagtcgtg ctgataaatg
     9601 attttgccaa agggatatct gatcattcct ctaaagagct tgctaatatc aatggcaaga
     9661 aaggaaaggt cgtgataaaa agggggaata ttgtgatatt ataagaaact tatttacatc
     9721 tatccatgcc ttaacttctg attttatata tgtgcatgtc gtttggctta aaattatcgg
     9781 taaacttacc atcataaacc tttaactgcc ttccttcatc ataaacatct gctatgccct
     9841 ctcttagggc aggtatagaa aaggtcgttt tgatatcttt tgtttctcca gtgccgccct
     9901 ttgggcttgt ctctgtaagc ctcaccgcaa agagccagat atcagtacca tttcttttta
     9961 ccatggtgtc gacccgatta cccggcacat ttgcagtgtc agatatggtt ataactgagt
    10021 caccgctaag cacaataggt gccaaatgtg tgatctgtga aacaaacttg cgcatggcaa
    10081 cgaagttccc gttaggtgag ggaagatgat aatggaacca gtttatccct tttgctccgt
    10141 ggacaacatt aagccatgca agcatcctta cctgctcagg cgtcggaggc cactgagtcg
    10201 agtaaggggg ttcgtttata tcagaagttt ctatccatga aaagacaggg gcaaggtctg
    10261 cattttcctg cctaatcttg tccattatct ctgcaagctg tgtgactgtc gctcctcttt
    10321 ggtgtggcaa agcccaatcg ataggataga aatcaaaacc taaaatgtcg gcaacaggtg
    10381 tcttttttcc cccaaaaagg cttgcatcat acagatattc atatttcctt ctgtgcgctc
    10441 tatcccaaac atcgccttcg gcaatccacc caggaccggc gaaatttgtt gcgacaggat
    10501 gctgagggtc accaaggtga gatgccaggg tccagctcct aagggcctgt gaagacactg
    10561 cttcataccc tccaagttca ggctcgtcca tccactgcca agccaaaata ttgctgtctt
    10621 ttgttgcatt aacatattcc atgaggttat ccagactcgt cacttttccc ttcaagccat
    10681 cccatcctga gggcccaagt acaagggtat tgtatttttt tgcttcatca aggtaatatt
    10741 tccagctctg aagatttttg gttgcaccaa atcctccgcc atgccagaag gctgttccgt
    10801 agagactgtt tatggccttg tctttgccta accacttggg gaagtccatg tcgtttaacc
    10861 cccagggcgt tactgggaaa aacaattcac catttaccct gatagcgtta ttttcatcga
    10921 taccaacttg ggggattcca tcatacggct tgtcaaaata ttctttgatc tcggcgatca
    10981 tattattaga tgaatcaagc agctgtgcac caagagtata gcttcctgcc tttagctctc
    11041 cattctttaa aagaaacctt tcttttttac tgaggcctct cttgtcgtat acaatagatt
    11101 catagccttt atttgaagat atcttcacaa ctagcctgct tgctgactta ctgattagct
    11161 cgtcaggaat gttagctgtg atatattggg ctgtccttgc atggaagtta gcatgttcca
    11221 gctcaaagcc atatagtact tcttcaatat caagagcttc ctgaacgatc tcaaactcat
    11281 ctatctcagc gttaaaaata ttatttcctg acttacttgc tccaagcact atattgccta
    11341 attctacatt accggcacaa gaggcagcct gtacaggttc cttgccgttg atatatagct
    11401 taagttttga gccgtcatag atgatagcat agtggttcca atcagtgttg tggcttaagg
    11461 tttcccagtt gctgagcagc gcacagtttc ccttgtctgt cttcacatca agagagagcc
    11521 tccattctga aacgtaagct agcgctaagc ttcctgcatc taagactacc cctccctttg
    11581 tcaggtcttt ttgccttgca aaaaaagata tggtgaactt attcagcgtt gtcggcttat
    11641 tctgtatacg cacaaatgag ccgccaaggt caaagcctct gtagacaggg ccataagtat
    11701 atttttggct gccttgataa gaggttaaga ttccagaagc ttcatttagg ctgccatcaa
    11761 agttcatcct gattatcgga ttgagaaggc ttatggtgct aattcttgca ggtatgccct
    11821 caacgctaat tggaacaatc gctttagtta tggttccctg cagattggtc atagttatag
    11881 ttacattata tttgccagga tacatatagg tgtgtgtaac gctgacgcct ccctctggat
    11941 acttaaggga ataagtgtcg ccaaagtccc aatcgaacct cgtcagagaa ggatcgctct
    12001 taaggtaact gaatgcattg aataatgctt cctctccagc ccttatatta tatgaagggt
    12061 acacctttag gggggattct tggcatgccg tgcattggct gctttttttg cattcaggat
    12121 ctgtatctat agagccatca caatcatcat cttttccatc tttgcaattg ccgtttgctg
    12181 cagattcctg taggttatac cctccgcagc tgcaagagca gtctttgcag tcgtcctttg
    12241 cagagacaca atcccctccc ctgcatgaat aatcgatgaa atggtccatc tcacagctat
    12301 ctttttggtt gcagtcagtg catgaagaaa ggccgcagga gttgtctgtt ccaggacata
    12361 aggcaggtgc gcactttgtg ttcagacaaa aattgctttc acagtctgat ttagatatgc
    12421 acgattttcc aagcccgcat ttgatgcagc cacttccaca gtctatatct gtttcaccct
    12481 ggttttgctt tctgtcaaag cacgttgctt tgtttatctc aaagctgttt atatcccatt
    12541 tagtcttatc tgatgacttt atgccgtagt aaaaccttgt tgaccgggct tttgccaagt
    12601 ctacagtcac cgattttttt gtcgtaatta tccaataaaa accccctggt gcgccgattc
    12661 tgtcatccgc acggatattc accataaaag gaggtctgcc gcttaatgtc aatgtgagtt
    12721 ttgtaccgtt taatgtgtga ggtgcctgag cagcagcaaa aaaagattca gcaagcaata
    12781 ttagtattat agtaaattaa attgaatatt tctcattaaa tggaataaga gatagaactt
    12841 tttgtttgtt gtaccaaaga cttctgaaga atttcataat tgccctttcc atttcaggaa
    12901 tagtaggata aaaccaattg ccaac
//
LOCUS       DASRUK010000081         9053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2073295, whole genome shotgun sequence.
ACCESSION   DASRUK010000081 DASRUK010000000
VERSION     DASRUK010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 9053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9053
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2073295"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..1124
                     /locus_tag="VFF28_03600"
     CDS             261..1124
                     /locus_tag="VFF28_03600"
                     /inference="COORDINATES: protein motif:HMM:NF025881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HZX44745.1"
                     /translation="MKYLDLPQKQQSYFLRTIKEKTSYSWSKIADILKVNRSMIFFYL
                     NEHSKLPYNHYLKLCKLANVEMNCKIGLLEINREIVIRHPTLSTTLAEFLGALAGDGH
                     VNNITFEVSISMDKDLDLYYSNHIVKMFTKLFGVKARKYTQNNKVKCFIYSKRLVFFL
                     SKRFRVPIGKKKDKLHIPKGVLRKKVLTRAYIRGLFDTDGSFHRHYKNDSMIGIISTD
                     RTFISEIRQALRGLGFVTSLNGKNLNIYRKKDIQRFFKEIKPSNKKHLTKYQYYVKNG
                     RVPLTNQILNR"
     gene            1160..1270
                     /locus_tag="VFF28_03605"
     tRNA            join(1160..1222,1236..1270)
                     /locus_tag="VFF28_03605"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1219..1221,aa:Gly,seq:tcc)
     gene            complement(1472..4948)
                     /gene="smc"
                     /locus_tag="VFF28_03610"
     CDS             complement(1472..4948)
                     /gene="smc"
                     /locus_tag="VFF28_03610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HZX44746.1"
                     /translation="MNKLVLRGFKSFAKHTELTFGPNYNCVLGPNGSGKSNILDSLCF
                     VLGKSSAKSMRAEKSSNLIYNGGKSKSPCKEGEVSIYFDNQNKRFPTDDPEVKITRIV
                     RQNGQSIYKINDKLRTRQEIVDLLSLAKIDPDGYNIILQGDITHFVEMHPVERRQLIE
                     EIAGISIYEEKKQKALLQLEKVDERLKEAEIVLAERKTYLKELKKDHDQAKKFKDMGD
                     NISINKATLLKLNIDKKESVKNDFQQSLEKARQELEAINKRIGDLKLENQKKKQEIEN
                     ISKEIEEKGEVEQVSLNKEIETIKIDLTKKNSRLETLESELTKIAKRREDLKASSLEI
                     EQKIADLKKQKEELTSRLKAQEAQKNELQSKINLIKDKNKLEELADVEKRIEDLDKQA
                     EELQKSTNGIREEQHNLIRQKDALDHQINTIDEQIKKVEELERENKKEIDSLKQKKEE
                     FRKSTLELNKCLNEDSSLAVQIGNSRDNVSKAMEELAKLQARSTTIRESTTADLAIQK
                     VLEHKGEGIYGTIADLGEVDTQYSLALEIAAGPRLKSIVVDNDKIAAEQIRLLKADRL
                     GIATFIPLNKIRSKDIDKGIEKLSKAKGCHGLAIDLIQFSQTYKKAFEYVFQDTLVVD
                     SIDAARRLGIGSARMVTLDGDLAEHSGLMQGGYRKKRAGMGFSEKQMNEDIERLGQEI
                     EETTNILHTLENRRKENEEMITRLREFKANLEGDILKVEKTLHLSPTDLEMSKVKKQD
                     ILKQSKDVEIKIDELISKISEKNKELAKNRIERQQLKNKTSALKAPELLAELAAFEEK
                     RNQLHEEITKLNGEINSIDMPVNIHQKEREKITGILKQIDKEEDQFKTESEYITKEAQ
                     KKQEELKNKESLAKEFHAKFRALFENRSKLSDELTKNDAQVSLKIDDSRKVEIRQNTL
                     TLKHAEAASELAGLQQEFQQYEGITLLLNVTEEQLKYEIAKFEKMKSEIGSVNMKALE
                     IYDAVEKEYASLTEKKDTLVKEKDDVVHMMNEIEDKKVDLFMKQYDAVCDNFKNIFSQ
                     LTTKGEAALELENKQNPFEGGLGVKVKLSGQKFLDIRSLSGGEKTLTALAFLFAIQEH
                     EPASFYIMDEVDAALDKRNSEKLAKLVAKYAEKAQYIIISHNDGIISEASTLYGVSMD
                     EHGMSKVVSLRV"
     gene            complement(5024..5488)
                     /locus_tag="VFF28_03615"
     CDS             complement(5024..5488)
                     /locus_tag="VFF28_03615"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZX44747.1"
                     /translation="MDSVPLRALVNSKETPSIETICTEFMISIIDTSAWVEYFEGSAK
                     GIFLKNLLEDKKNKFVTMESCIAELVGVSLRQGLDFRQLKTFVKENSVILPILTETWI
                     SAAKIRHVVRKTRPQFGLLDALLLAKQKELNCSLISSDSHFKALKNVVYIGD"
     gene            complement(6411..6734)
                     /locus_tag="VFF28_03620"
     CDS             complement(6411..6734)
                     /locus_tag="VFF28_03620"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HZX44748.1"
                     /translation="MKDDSVYIAHILESIERIEQYLSGISKEKFMESGIIQDAVIRQL
                     AIIGEASKHISKELKNEIELPWKDIAGMRDRLIHAYFGVNLDEVWKTIKEDLPFLKDK
                     IKDFC"
     gene            complement(6731..7009)
                     /locus_tag="VFF28_03625"
     CDS             complement(6731..7009)
                     /locus_tag="VFF28_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZX44749.1"
                     /translation="MDKRLERTVVQHLKKQGAKSIAVFGSFARDEQKKGSDLDILVEF
                     KEIKSLLEMSRIERELSKKAGIKVDLLTKKGISPLIYSHIKKDLKVIL"
     gene            complement(7061..7942)
                     /gene="rnhB"
                     /locus_tag="VFF28_03630"
     CDS             complement(7061..7942)
                     /gene="rnhB"
                     /locus_tag="VFF28_03630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HZX44750.1"
                     /translation="MGPVIGPMVIAGVLVNEEDIPKLKSMGAKDSKLLTRKQRDDLFE
                     PIKQMVKAYKIIVIPAEEIDRALFSDDLNLNWLEAHKSAEIITELKPERAVIDSPSNN
                     CKAYTLYVEKLLAKTHCNNSLFNHDHHKIEVICTHKADVKFPEVSAASILAKVTRDRE
                     MDEICKKYGDAGPGYMSNPITQKFLKENWDKHPEIFRHSWMSYKNHEEQRFQQTFTDF
                     ANVVEKQEGMVTKEDLKALEAYGYQFTEAKGEHEVARLKGPCTITLYTTGKLLIQGKP
                     DKKEEVEKVLRKAGWVG"
     gene            complement(7999..>9053)
                     /locus_tag="VFF28_03635"
     CDS             complement(7999..>9053)
                     /locus_tag="VFF28_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZX44751.1"
                     /translation="WGDGKFMASVGHITLEKAKEYLEKQETHHANNSTESPPFRVGVE
                     VNLFHKTVSSLFGHLKLKIRIYVYNPSIGNHKVKLANAKARFYLDKRANQTYYIWRLA
                     STKIVEEWKGRVQTITGNHQYYASILRGFFAGEGNIKTGSHSNRTIRIAQGKPFPLLE
                     KILTKFKVDFKYSLAERSYVIVGKRNWDVLAQLGIADLHPIKKTKFWNTYNSYKEEHY
                     SANYLKTKILQLLEKPYTSQQLAGIMGRSQARVYDILGNLKQLGIVKNYRVGSKDYWI
                     RKDQNIIILSLTKQKYLSYLNRSAMSTNNLCNKLKVGWKCSFRRLNELKRLGLVKRNE
                     DKTWKRLKIDKKVICL"
BASE COUNT         2315 a         1919 c         1607 g         3212 t
ORIGIN      
        1 tctgattcaa gggtaaattt gccatcttgg aacttgaatt tacgcatgat atatattaat
       61 atatactata actaaataaa gatttcggtg atggaacata tagtcctaag aagttagttt
      121 atatatgact gaatacttac agaagatgaa agagataaag aaagtaaatc actaaattta
      181 taaataaaat aaggattctg gtctaacggg tatgatgtaa accgcaaagt taatataact
      241 ctctacaatt aaatacctag atgaaatatc tagacttacc tcaaaagcag caatcatatt
      301 ttcttagaac cataaaggaa aaaacaagtt attcatggag taagattgct gatatattaa
      361 aagttaacag aagcatgatt ttcttctatc taaatgagca ttccaaactc ccctataacc
      421 attaccttaa actttgtaaa cttgccaatg tagagatgaa ttgcaaaata ggattattag
      481 aaatcaatag agagatagta atcaggcatc cgacattatc gacaacactt gctgaattcc
      541 tcggtgcatt agcaggcgat ggtcacgtaa ataatataac atttgaagta tctatctcca
      601 tggacaaaga cctagactta tactactcaa accatattgt gaagatgttc actaaattgt
      661 ttggggttaa agccaggaaa tatacccaaa acaataaggt taagtgcttt atttattcca
      721 aaagattggt gttcttttta agcaaaaggt ttcgtgttcc tatcggcaaa aagaaggata
      781 agctccatat ccctaaggga gtcctaagaa agaaagtatt gacaagggct tatataagag
      841 gactatttga tactgatggc tctttccata gacattataa gaatgactcc atgataggaa
      901 taattagcac agaccggaca tttatctctg agataagaca agcattaagg ggattgggct
      961 ttgtaacttc tctaaatggc aagaatttga acatatatag aaaaaaagat attcagcgtt
     1021 tcttcaaaga aatcaaacca tccaataaga aacaccttac caagtaccaa tattatgtca
     1081 aaaatggcag ggtaccatta acaaaccaaa tattaaatcg ttaagtttat ataaatagcc
     1141 ttagtcctga agattgcgcg cgccggtggt ctaatggtta caattagcac agctaattgc
     1201 cataaatgac ttcagccttc cattaaccag aagcaagctg agtatccggg ttcgaatccc
     1261 ggctggcgca tccatctttt tatcactttt tgccaacttt acactttctg atcatcacgg
     1321 gaactattgt tccctacacg ggtatctaca tttagatagg gtatctgcat tcctgaatat
     1381 tcttagggaa aggttggctt aaaagagata agggaaaagc cttccatcag ggttcattat
     1441 ttattcaagg gttaataata tcctcaacac ttcaaaccct taagctaaca accttggaca
     1501 tcccgtgctc gtccattgat actccataga gtgtggaagc ttctgatatg atgccgtcgt
     1561 tatggctgat gattatatac tgggcttttt ctgcgtattt tgcaacaagc tttgcaagct
     1621 tttcggagtt tctcttgtca agtgctgcat ccacttcatc cattatgtag aatgatgctg
     1681 gctcatgctc ttggatggca aagaggaatg caagcgctgt caaggtcttt tctccgcctg
     1741 acaaagacct gatgtcaagg aatttctggc cagacagctt tacctttact ccaaggccgc
     1801 cttcgaaagg gttctgcttg ttttccagct ccagggctgc ttctcctttt gtcgtgagct
     1861 gagagaaaat gttcttgaag ttatcgcaga cagcatcata ctgcttcatg aagaggtcaa
     1921 ccttcttatc ctctatctca ttcatcatat gtacaacgtc gtctttttct ttgactaatg
     1981 tgtctttctt ttctgtaaga gaggcatatt ctttttcgac cgcatcgtat atctccagag
     2041 ccttcatgtt gacagagcct atctctgact tcatcttttc aaactttgct atctcatatt
     2101 ttaattgctc ttctgtaacg tttaggagaa gggtaatgcc ttcatactgc tggaattcct
     2161 gctgcaagcc tgcaagctct gatgctgcct ctgcgtgctt taaggtaagg gtgttttgcc
     2221 ttatctcgac cttcctgctg tcgtctatct tgagcgagac ctgagcatca tttttggtta
     2281 gctcatcact tagcttggaa cggttttcaa agagagccct aaactttgca tgaaactctt
     2341 ttgcgagact ttctttattt tttaattcct cttgcttctt ttgggcctct tttgttatat
     2401 attctgactc tgtcttaaac tggtcttcct ctttgtctat ctgctttagg atgcctgtta
     2461 tcttttctct ttctttctgg tgtatgttca ccggcatatc tatagaattt atctcgccat
     2521 taagcttggt tatctcttca tgcagctggt ttcttttttc ctcaaaagct gcaagctctg
     2581 ccaagagctc aggggctttg agcgcggatg tcttgttttt tagctgctgc cgttctatcc
     2641 tgtttttggc aagctccttg tttttttccg agatcttgct gataagctcg tcgatcttaa
     2701 tttcgacgtc ttttgactgc tttaagatgt cctgcttttt tacctttgac atctcaagat
     2761 ctgttggact taggtgcagg gtcttttcca ccttcagtat gtcaccctca aggtttgcct
     2821 taaattctct aagccgggtt atcatttctt cgttttcctt ccttctgttt tccaaggtgt
     2881 gaaggatatt agtcgtctcc tctatctcct ggcctaatct ctcaatgtct tcgttcatct
     2941 gcttttcgct gaagcccatg cctgcacgct tctttctata accgccctgc atcaggcctg
     3001 agtgctcggc caggtcacca tctaaagtca ccatccttgc gctgcctata cccagccttc
     3061 ttgctgcgtc tatgctgtca accaccaatg tgtcctggaa aacatattca aaggctttct
     3121 tgtatgtctg actgaactgg ataaggtcga tggcaaggcc atggcagcct tttgcttttg
     3181 agagcttttc tatgcctttg tcaatatctt ttgaccttat cttgtttaga gggatgaaag
     3241 tagctatacc taatctgtct gcttttagca gccttatctg ctctgctgca atcttgtcgt
     3301 tgtctacaac gatgcttttc aaccttgggc ctgctgctat ctccagggcc aaggaatatt
     3361 gagtgtcgac ctcccctagg tctgctatcg tgccgtatat gccttcacct ttgtgttcca
     3421 acactttctg gatggcaagg tctgcagtgg tcgactccct tatggtcgtg cttcttgcct
     3481 gaagctttgc aagctcttcc atggcttttg aaacattatc tcttgagttt cctatctgta
     3541 ctgctaatga ggagtcttca tttaggcatt tgtttagctc taaagtagat ttcctgaact
     3601 cttccttttt ctgttttaag gagtcgatct ctttcttgtt ttctctttct aactcctcta
     3661 ctttctttat ctgctcatct atagtgttta tctgatggtc aagggcgtct ttttgcctga
     3721 tgaggttgtg ctgctcttct cttatcccat tagtcgactt ttgaagctct tctgcctgct
     3781 tgtctaagtc ttctatcctt ttttctacgt cggcaagctc ttcaagcttg ttcttatcct
     3841 ttatgagatt gatctttgac tgcagttcgt tcttttgggc ttcctgcgcc tttaatcggc
     3901 tggtcagctc ttctttctgt ttttttaggt ctgctatctt ttgctctatc tcaaggcttg
     3961 aggctttaag gtcttctctt ctttttgcaa tcttcgttag ctctgattct aatgtttcaa
     4021 gccttgagtt cttctttgta aggtcaatct ttatcgtctc tatctcttta ttaaggctga
     4081 cctgctcgac ctctcctttt tcttctatct cctttgagat gttttcaatc tcctgcttct
     4141 ttttttggtt ttcaagcttt aggtcgccta tccgcttatt tatcgcttct aattcctgcc
     4201 ttgctttttc aaggctttgc tgaaagtcat tttttactga ctctttcttg tcaatgttga
     4261 gcttgagaag ggtggctttg tttatagaga tattatcgcc catatccttg aacttctttg
     4321 cctggtcatg gtctttcttt agctctttta gataggtttt tctttctgca aggactatct
     4381 ctgcttcttt taggcgctca tcaacttttt ctaattgcaa gagagctttc tgcttttttt
     4441 cttcgtatat ggagatgcct gctatctctt ctatcaattg acggcgctct actggatgca
     4501 tctctacgaa gtgggttata tctccttgaa gaattatgtt atagccgtct gggtcaatct
     4561 ttgctaaaga caggaggtct actatctctt gtcttgtcct taacttatca tttatcttgt
     4621 agatggattg gccgttttgc cttactatcc tagtaatttt tacctcaggg tcatctgtcg
     4681 gaaaacgctt gttttgattg tcgaaataaa ttgagacttc cccttcctta caagggcttt
     4741 ttgattttcc tccgttatat ataaggtttg aggatttttc agccctcatg gattttgcgc
     4801 ttgattttcc cagaacaaag cataagctgt cgagaatgtt cgatttgccg ctgccgtttg
     4861 gacccagaac acagttatag ttagggccga aagtaagctc tgtgtgcttt gcaaagcttt
     4921 tgaagccgcg gagtactaat ttattgatat gagtaactta aatcaccttt ttattgacac
     4981 taaagaagta aaatccgttc aaggttaata aagattttga ttatcaatcg ccaatgtaaa
     5041 cgacgttttt tagggctttg aaatgtgagt cactactaat taaactgcaa tttagctctt
     5101 tctgttttgc caataatagg gcatcaagta agccaaactg cggccttgtt tttctcacta
     5161 catgacgaat ctttgcagcg cttatccatg tctctgtcag aattggcaaa ataactgaat
     5221 tttccttaac aaaggttttt agctgtctaa agtccagacc ttgccttaga gagaccccaa
     5281 ctaattcagc gatacagctt tccatggtga caaacttgtt ttttttatct tccagtaaat
     5341 tcttaagaaa tatgcctttt gcactgcctt caaagtattc cacccatgcc gaagtatcta
     5401 ttatggatat cataaactca gtacaaattg tctcgatgct cggtgtctct ttggaattta
     5461 ccaatgccct taaaggcacc gaatccatct ctcctgttac gtttaattat gtcaacttta
     5521 atagtatcac ctttattcag tccatcatgc tcaattattt cttttggaat gattatccca
     5581 agtgaatttc cccactcttt aacctgaact tctgccatgg tcataaacat cacctagtta
     5641 tactaaaatg tataactgat atattaaggt tttgaaatat tccagagata gtgaaaatat
     5701 tttctatatg aatccgctat atccttattt attaataaaa agcatagcgg attgttgtct
     5761 ttccaaacaa catttaccac ccgcacaggg aaccacgtca agctagttct gtgtattata
     5821 ttaaacgtgt tgatcttgat agcatgtgta actccaatga attattctgg acttttatgg
     5881 acaatttggc agaagttaca aatgatgcgc ataaaacaat ataggagcgt tatgcaagaa
     5941 ataaggaacg tttagatagt ccccctgtta tagtaaggaa agaaccagtt taaacgcact
     6001 tatacaagcg ttcgaagcct taaagggtga ttactcttta tccaaccagt caatcccttc
     6061 ctgaaatatt taaaggtaaa attatgccac gactaactga agtattatcc actttctttt
     6121 tttttcgctt aatgtttcca tgagcctcac cctaagcaga ggaatattgg ttaattatta
     6181 aatgtgtaaa caaattatga acttgaaaaa aatattatca ttttggtaaa ccctatatac
     6241 aagaagcgat gatctacccc cagcagacta attgatgaat ggcagatgat tgctatcatt
     6301 gtgtataatg aattcacaga aagaaaaatc cagtaagtgc aatatcttga agttacccaa
     6361 cccatccagc cttccttaga actcttttga cctcttctta cttgaaataa tcagcagaaa
     6421 tctttaattt tatcctttaa gaaaggcaaa tcttccttta tcgtcttcca tacctcgtct
     6481 aagttaactc caaaataagc atgaataagt cgatccctca tgccagcgat gtctttccaa
     6541 ggcagttcaa tctcgttctt taactcttta gatatatgct ttgatgcttc accgatgatt
     6601 gccagctgcc tgataacggc atcctgtata atgccacttt ccataaattt ttcctttgaa
     6661 ataccggata ggtattgttc aatcctttct attgattcaa gaatatgtgc tatgtataca
     6721 gaatcatcct ttatagaatc actttaagat ctttcttgat atggctgtat ataagagggc
     6781 taatcccttt ttttgtaagc aagtcaactt taattccagc ttttttggaa agctctcgtt
     6841 caatccttga catctcaaga agacttttta tttctttaaa ctcaaccagg atgtccaaat
     6901 cggagccttt tttctgctca tccctcgcaa atgagccgaa taccgcaatg ctttttgccc
     6961 cttgcttttt caaatgttga acaactgttc tctcgagcct tttgtccata gatgagagat
     7021 atctgcttaa aaagatataa agctttcgtt gttgaaaaat ttaaccaacc catccagcct
     7081 tccttagaac tttttcaacc tcttctttct tatcaggctt tccttggatc aagagctttc
     7141 ctgttgtgta aagagtgatc gtgcatgggc cttttagcct tgccacctcg tgctcgcctt
     7201 tggcctctgt gaattggtag ccgtaagctt ccagggcttt caggtcttct tttgtaacca
     7261 taccttcttg cttttccact acatttgcaa aatctgtaaa tgtctgctgg aatctctgct
     7321 cctcatggtt cttgtaagac atccagctat ggcggaatat ctcaggatgc ttgtcccagt
     7381 tttccttgag gaacttttgg gtgatcggat ttgacatata gcctgggcct gcatcgccat
     7441 attttttgca tatctcgtcc atctccctgt ctcttgtgac ttttgccagg atagatgctg
     7501 cagacacctc tgggaacttg acatctgcct tatgagtgca gataacttct atcttgtgat
     7561 gatcatggtt gaacaaagag ttattacagt gggtttttgc caggagcttt tcaacgtata
     7621 atgtgtatgc tttgcagtta ttggagggac tgtctatcac tgctctttct ggctttagct
     7681 cagttattat ttctgctgac ttgtgggctt ccagccagtt aaggttaagg tcgtcagaaa
     7741 ataaggcgcg gtctatttct tctgcaggga taactataat cttatatgct ttgaccatct
     7801 gctttatcgg ctcaaagagg tcatcccttt gctttcttgt aagaagcttt gaatcttttg
     7861 cgcccatgga tttaagcttt ggaatatcct cttcgtttac taatacgcct gctatcacca
     7921 tcggaccgat tacagggcct attggcgagt taacctaagt taactctctg ccagcctcat
     7981 cgatgccgca cactaatgtc ataaacaaat caccttttta tctattttta atctcttcca
     8041 agttttgtcc tcatttcttt taactaagcc taatcttttt aattcattga gtcttctaaa
     8101 agagcatttc caaccgactt ttaatttatt acaaaggtta tttgtgctca tagctgacct
     8161 gttgagataa ctaagatact tctgcttagt aagggacagt ataataatat tttggtcttt
     8221 ccttatccaa taatctttac ttcctaccct atagttctta acaattccca attgctttag
     8281 attgccaagg atatcataga ctctggcttg gcttctaccc atgatacctg ccagctgctg
     8341 agaggtgtag ggcttctcca ataattgcaa tatcttggtc tttaggtaat tagcagagta
     8401 atgctcttct ttataactat tatatgtatt ccaaaacttt gtttttttta ttggatgtaa
     8461 atcagctatt cctaactggg ccaatacatc ccagttgcgt ttaccaacta ttacataact
     8521 tctttcagcg agggaatatt taaaatctac cttgaatttt gttaatatct tttccagcaa
     8581 aggaaacggt ttaccttgag caatcctgat agttctgtta ctatgggatc cagtttttat
     8641 attgccttca ccagcaaaaa agcctcttaa aatgcttgca tagtattggt gattgccggt
     8701 gattgtttgt actctgcctt tccattcctc aacaatttta gttgaggcaa gtctccaaat
     8761 atagtaggtt tggtttgccc ttttatccaa gtagaatctg gctttagcat ttgctaactt
     8821 aactttatgg ttccctatag aaggattgta gacataaatt ctgattttta gctttaagtg
     8881 tccaaaaagg ctgcttactg ttttatgaaa taaattgacc tccaccccaa ccctgaaggg
     8941 tggggattcc gtcgaattat tggcatgatg agtctcttgc ttctctagat attctttggc
     9001 tttttctaag gtgatatggc caacagaagc cataaacttc ccatcacccc aaa
//
LOCUS       DASRUK010000082         7763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1074247, whole genome shotgun sequence.
ACCESSION   DASRUK010000082 DASRUK010000000
VERSION     DASRUK010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 7763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7763
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074247"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VFF28_03640"
     CDS             <1..957
                     /locus_tag="VFF28_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44752.1"
                     /translation="FRAYCLLDYRTTETSTTGPFGLGASMLSLFITLSLGIALGCYYR
                     LRSSNVIEIRKKTDELEQEFASGLFQLGNRLGDGLPAETAFGKVSQAMQGTVSGSFFQ
                     IVSLNIRRLGMGVTEAIFDPRHGAVAYYPSNLIESSMKVLIESVKKGPKVASMALMNI
                     SRYIKEIHKVNERLKDLMAEVISSMTSQIKFLTPVIAGIVIGITSMITTILGKLAVQM
                     KQMNADAGADATSAMMSGFFGQGVPTYYFQIIVGLYVVQITWILTILANGIENGSDKL
                     NERYLLGKNLIRSTLIYCIVAGAIMLIFNSIANQIMFVTLTA"
     gene            complement(1239..1673)
                     /locus_tag="VFF28_03645"
     CDS             complement(1239..1673)
                     /locus_tag="VFF28_03645"
                     /inference="COORDINATES: protein motif:HMM:NF044805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_component: GO:0070876 - SOSS complex [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOSS complex subunit B family protein"
                     /protein_id="HZX44753.1"
                     /translation="MAIKDLQARQGQVDLIADVVEKAEPREFEKFGKKGKVCNAKIKD
                     ETGEMTLTLWNEQTETVKVGDKVHVINGWVSEWQGEKQLSTGKFGQLEVVEKGTAVPA
                     PSKAPEKNASAMPKQPERKEVNYEDIEKETDDEVYKDEEEVY"
     gene            complement(1675..3105)
                     /locus_tag="VFF28_03650"
     CDS             complement(1675..3105)
                     /locus_tag="VFF28_03650"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HZX44754.1"
                     /translation="MTLTIDDMSVFCKKKGFVYPSSEIYGGFSGFFDYGPLGVELKNN
                     IKAAFWKRFVTDRENIVGIDGTIVTHPMVWKASGHVDNFTDYIYTCGKCKTMIKGEEK
                     EEVKCPKCKGNMEKQGKFNLMFETNVGPVKGNTAYLRPETAQVIFADFKQVMDSSRQK
                     LPFGIAQIGKAFRNEISPRDFLFRCREFEQFEIEFFVHPNKVNDCPNIAEVMDINVNL
                     ITAENQEKKKDHFIISIKEMIDKKICSAWHAYFLAEYYQFFLDMGIKPENLRLREHLK
                     DELAHYASACFDIEYKFPFGWKEIHGNADRGQFDLKQHMQFSKTNMEYFDEETKEKVI
                     PYVASEPSQGIDRAFLAFLYDAYNDDKERGNIVLKLKPSLAPIKVGVFPLVNKLNDKS
                     REVFEVLRKEFNCIYDKSGSVGRRYARADEMGIPFCVTVDFDTLNDESVTVRDRDSTK
                     QVRVKINKLASVLHDMIIGKKHFEGG"
     gene            3182..3811
                     /locus_tag="VFF28_03655"
     CDS             3182..3811
                     /locus_tag="VFF28_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44755.1"
                     /translation="MKLIILFLFAFLAGCASTDEISQIDKAQEQEAVMPSGNCTQGWI
                     CSDLNKKGYQFQDCIVAQETICQNGCKDGECLTLETKQAKTYTITQGMGRMQEISIKS
                     VDFSENIFLDIGAPKLDVSIKLSAPYIGYTFFTAESPGRNLWIIDKKITEAGRKDCID
                     TIADAAAIASFYSGKTLCIKTTEKNIALVGGFWQGAPDENTWLSWKYYS"
     gene            3897..4031
                     /locus_tag="VFF28_03660"
     CDS             3897..4031
                     /locus_tag="VFF28_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44756.1"
                     /translation="MKEKISITIDEKSLFALRDNIRQQRHKSKSEAVEFCLRSLLEEF
                     "
     gene            4152..4286
                     /locus_tag="VFF28_03665"
     CDS             4152..4286
                     /locus_tag="VFF28_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HZX44757.1"
                     /translation="MKTKLSISIDSSLLQKIDNLVELGIFRSRSHAIDFSIVGAKYGR
                     "
     gene            4276..5046
                     /locus_tag="VFF28_03670"
     CDS             4276..5046
                     /locus_tag="VFF28_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44758.1"
                     /translation="MAGEDLEKRLAPQGGSELEKKAEKSDWTFWNVARDVALYSPLAV
                     LAYAIGGPASLLTPIGLGIGKYLTNRKKKQKTTWKDMRRTLAVGNFGGALAYWAYKVP
                     DLIMGTPLGLTGMIAKTLLFNPIMLAPWMAWYRTTNYIVGKYGVWGTIKSMFDGKIFG
                     YIKEAYNEDLKKKYWSTMAEAFLTISPIHFYSMNYLHNPTYRVALGTVNDVILSTIAG
                     EEGLLRNIKKKLIYTAPEKEPNIVKFPTAEPQYLSKTG"
     gene            complement(5047..6249)
                     /locus_tag="VFF28_03675"
     CDS             complement(5047..6249)
                     /locus_tag="VFF28_03675"
                     /inference="COORDINATES: protein motif:HMM:NF003229.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication factor C large subunit"
                     /protein_id="HZX44759.1"
                     /translation="MIPFTKKYDPKCCTDVKGQDKAISEIRELAKNYSKGKKAIWLYG
                     PSGCGKTSLVHALASELSYEIIEVNASDTRNKEEIWQKLGGAALQMSLFSKGKIILVD
                     EVDGLSGMEDRGGLAAVADIIERSAFPIILTSANPWDYKFNALRKKCTLIELEGLNYA
                     EIAEILRGICEKEKIRCDIEVLKNLARRAGGDCRAAINDLQTLTQIGREITKESIEEL
                     FERSQEESMPSALLKIFKTTDPKVAITAFDNITEDLDHQMLWLDQNLPLEYEKPEDLA
                     RAYDKLSKADVFNRRIRRWQHWRFLVYINALITAGVAVSKDEKNHKFVQYKPTGRLLK
                     LWWAKQKSMKKRAIAEKIAEKTHSSTREVIKDIEYFKVIFKKDKEMADKIAVYADLDK
                     EEVDWLRK"
     gene            complement(6323..6451)
                     /locus_tag="VFF28_03680"
     CDS             complement(6323..6451)
                     /locus_tag="VFF28_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44760.1"
                     /translation="MTKLLDMSEIASGARSDALAQSKPAISEHAQEHAPGFQPGGS"
     gene            6454..6996
                     /locus_tag="VFF28_03685"
     CDS             6454..6996
                     /locus_tag="VFF28_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZX44761.1"
                     /translation="MLVNIVKADIETFRLHQGRVLDIYMETATSGVMQQTLSRDTELK
                     YMLGLFEADGHGYLAFDGGNLIGFMLSGALGHDDLLPGTVRERFPVDRCYYISEMHVA
                     KEYQGKGIGSQMMQKCLDEIDRHRWDYLFIRAWKENEGALKLYQRFGFKLDEIIEQEK
                     IKKDRSSTFIIQKQYLWKKL"
     gene            7066..>7763
                     /locus_tag="VFF28_03690"
     CDS             7066..>7763
                     /locus_tag="VFF28_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZX44762.1"
                     /translation="MELPKLYDPKVSEPKWQKFWEENDIYRFDQKSDKEIFSVDTPPP
                     TVSGKMHLGHAFSYSQQDFVVRFQRMQQKNIFYPFGTDDNGIPTERLIEKTKNVKAFQ
                     MKRQEFIDLCLETLDKELRPKYIADWKRIGMSCDWNLNYTTINKHCQKISQKSFIELY
                     GMDRAYRKEAPVMWCPDCQTGISQVECQDKLLDSYFNDIVFKVDGENLIIATTRPELL
                     PACVAVFFHPDDKR"
BASE COUNT         2263 a         1703 c         1571 g         2226 t
ORIGIN      
        1 tttagggcct attgtctcct ggattacagg acaactgaaa caagcacgac aggcccattt
       61 gggcttggcg cttccatgct cagccttttt atcaccttaa gccttggcat agccctaggc
      121 tgctactata gattaaggtc cagcaatgta atagagataa gaaaaaagac agacgagctt
      181 gagcaggagt ttgcatcagg cctcttccag cttggaaacc ggcttggcga cggccttcct
      241 gcagagacag catttggaaa agtaagccag gctatgcagg gcactgtctc aggctctttc
      301 ttccagattg tttcgcttaa catcaggagg cttggcatgg gtgtgacaga agcgatattt
      361 gaccctaggc acggcgcagt cgcctattat ccatctaatc tcatagaaag ctccatgaaa
      421 gtgctcatcg agtcagtaaa aaagggccca aaggtagctt ccatggctct gatgaatatc
      481 tcaagataca taaaagagat ccacaaggta aacgagcgcc taaaagacct catggcagaa
      541 gtcatctcat ccatgacctc gcagataaag ttcctcaccc cagtgatcgc aggcatagtc
      601 ataggtataa caagcatgat aacgacgatt cttggaaagc ttgctgtcca gatgaagcag
      661 atgaatgctg atgcaggcgc agacgcgaca agcgcaatga tgtcaggttt ctttggccag
      721 ggcgttccca cttattactt ccagataatc gtcggcttat atgttgttca gattacctgg
      781 atattgacaa tacttgcaaa tggcatagaa aacggctcag acaagctcaa tgaaaggtat
      841 ttgcttggaa aaaacctgat aaggtcgacc ctcatctatt gcatagtcgc aggagcgata
      901 atgctcatct ttaattctat agcaaaccag ataatgtttg tcacactcac agcttaaaag
      961 aaaaaagtgg ggacggggga actttcaaat cctgagggat tgtttccctc tgggatattt
     1021 gaatccccga tttaaggaag cccactttta aggttgaact tctcacctac gtcttcagcg
     1081 tagtttaaga tcataatgga tgctctcctt agccgaagac aactaagcct tctcaggctg
     1141 agctacgttt atggttctta aaaattaatt ctaagaatcc ccattatgcc caaccgtacc
     1201 tcacagaatt ggataattct atataaagat tgttgttttc agtaaacctc ttcttcatcc
     1261 ttatagactt cgtcgtcagt ctctttctct atgtcttcat agttgacttc ctttctctct
     1321 ggctgtttag gcatcgctga ggcgttcttc tctggtgctt tgctgggtgc aggaacagca
     1381 gttccctttt caaccacttc cagctgccca aactttccag tagaaagctg cttctctcct
     1441 tgccattcag agacccatcc attgatgaca tggactttat cgccaacttt gacagtctcg
     1501 gtctgttcgt tccaaagagt aagtgtcatc tcgccagtct catcctttat cttggcattg
     1561 catacctttc ctttcttgcc aaacttctca aattccctcg gctcagcctt ctcaacaaca
     1621 tcagctatta gatcaacctg tccttgcctt gcctgtaagt cttttattgc catatcaacc
     1681 accttcaaag tgtttttttc ctattatcat atcatggaga acgcttgcta atttattaat
     1741 ttttaccctc acctgctttg tgctgtccct atccctgaca gtgacgctct catcattcaa
     1801 tgtgtcaaaa tcaacagtaa cgcagaaagg tattcccatc tcgtctgccc ttgcatacct
     1861 tcttcctaca gaaccgcttt tgtcatagat gcagttaaac tctttccgca aaacctcaaa
     1921 cacttccctg gatttatcgt tcaatttatt tacaagaggg aaaactccga cctttatagg
     1981 tgcaagtgaa ggctttaatt tcaagacgat attccctctc tctttgtcat cgttataagc
     2041 atcataaagg aaagcaagaa acgctctgtc tataccctgt gaaggctcag atgcaacata
     2101 agggataacc ttctcctttg tctcttcgtc aaaatactcc atatttgttt ttgaaaattg
     2161 catgtgctgt tttaggtcaa actgccctct atctgcattt ccgtgtatct ctttccatcc
     2221 aaaaggaaac ttatattcga tgtcaaagca agcagaggca taatgcgcaa gttcatcctt
     2281 aagatgctcc cgcaggcgta gattctcagg ctttatcccc atatccaaga agaactgata
     2341 atattcagcc agaaaataag catgccaggc agagcagatc ttcttatcta tcatctcttt
     2401 tatgcttatg ataaaatgat ctttcttctt ctcctggttt tcagcagtga taagatttac
     2461 atttatatcc attacttcag cgatatttgg gcaatcgttt actttattcg gatggacaaa
     2521 aaattctatc tcaaactgct caaactccct gcatctaaaa agaaaatcac gcggcgatat
     2581 ctcgtttcta aatgcctttc ctatctgtgc gattccaaaa ggcagctttt gcctggaaga
     2641 atccatgacc tgcttaaagt cagcaaagat tacctgtgca gtctcaggcc taagataagc
     2701 agtattgccc ttgacaggcc ctacatttgt ctcaaacata aggttgaact tgccctgctt
     2761 ttccatattt cccttgcact ttgggcactt tacctcttct ttttcctctc cctttatcat
     2821 tgtcttgcat ttgccgcagg tatagatata atcagtaaaa ttatcaacat gcccagatgc
     2881 cttccagacc atgggatggg tgacgatagt cccatcgata cccacgatat tttcccgatc
     2941 tgttacaaat ctcttccaaa aagcagcctt tatgttgttc ttgagttcaa ctccaagcgg
     3001 cccatagtca aaaaagccag aaaacccgcc gtatatctcg ctgcttggat acacaaatcc
     3061 tttcttcttg caaaacaccg acatgtcatc tattgtcaaa gtcataagtt gctaaaaatt
     3121 tataactata tataaatatt gccctcaaaa cttttatata atagcagagg gataaaaagg
     3181 tatgaaactc ataattctat tcttatttgc ttttcttgca ggatgtgctt ctacagatga
     3241 aatttcccaa atcgataaag ctcaggaaca ggaggcagtt atgccatcag ggaattgcac
     3301 ccagggctgg atctgctctg acctaaacaa gaaaggctac cagttccagg actgcatcgt
     3361 ggcccaggag actatatgcc aaaacgggtg caaagacggt gaatgcctta cccttgaaac
     3421 caaacaggca aagacctaca ctattactca aggtatgggc aggatgcagg agatttccat
     3481 aaaaagcgtc gactttagcg agaacatttt tttggacata ggcgcaccta agcttgatgt
     3541 aagcattaaa ctgagtgctc cgtacattgg ctacacattc ttcactgctg aaagtccagg
     3601 caggaatctg tggataattg acaagaagat aacagaggca ggaagaaaag actgtatcga
     3661 cactatcgca gatgcagctg ccattgctag cttttattca ggaaagacct tgtgcatcaa
     3721 gacaacggag aaaaatatcg cattagttgg cggcttttgg cagggcgctc ctgacgaaaa
     3781 cacctggctt tcctggaaat attattctta gttgataagt attttaccac taaacaatag
     3841 tataattagc gatagcttta tatattagta gtatgtcata ctaccactag gtagtaatga
     3901 aagaaaagat aagcataacg atcgatgaaa aaagcctgtt tgccttaagg gacaatatta
     3961 ggcagcaaag gcacaaaagc aaaagcgagg cagttgaatt ctgcttaaga agccttctag
     4021 aggagtttta gatgtgtcag aaatcactat tggcgcacac ttttggtgtt ttaacacaga
     4081 gtaagccagt gatttctgag cacgctcaaa aactccacca tcgcgctccg cattttaatg
     4141 aggagtttta gatgaaaaca aaactcagta tatccataga cagctctctc cttcagaaga
     4201 tagacaatct ggttgagctt ggcatattta ggagcagaag tcatgccatc gacttctcta
     4261 tagtgggtgc aaaatatggc aggtgaagac ttagagaaaa ggcttgcacc tcaaggtggc
     4321 tctgagcttg aaaaaaaggc agagaaaagc gactggactt tttggaacgt cgcaagggat
     4381 gttgctcttt actctccgct agcggtttta gcttatgcga taggaggtcc tgcaagccta
     4441 ttgacgccta ttgggctggg gataggaaaa tatcttacta ataggaaaaa gaaacaaaag
     4501 accacatgga aagatatgag aaggacgctt gctgtgggaa actttggagg agcattggca
     4561 tattgggcct acaaagtgcc tgacttgata atgggaactc cattagggct gacagggatg
     4621 atagcaaaaa cccttttatt taatcctata atgcttgccc cttggatggc atggtatagg
     4681 acaactaatt acatcgttgg aaagtatggg gtatggggaa cgataaaaag catgtttgat
     4741 ggcaaaattt ttggttatat aaaagaagct tacaacgaag acctaaagaa gaagtattgg
     4801 tcaaccatgg cagaagcctt ccttacaata tcccctattc atttttatag catgaattat
     4861 ttgcacaatc cgacttatag agttgctctc gggacagtaa atgatgttat cctatcgacg
     4921 attgctggcg aagagggtct tttgagaaac atcaagaaaa agcttattta tactgcccct
     4981 gaaaaagagc caaacattgt taaattcccc acagccgagc ctcaatatct ttctaaaaca
     5041 ggctagctat tttctaagcc agtcgacctc ttctttatca aggtcagcat atactgctat
     5101 cttatcagcc atctctttgt cctttttgaa tataaccttg aagtactcga tatctttgat
     5161 aacttccctg gtgctgctat gcgtcttttc agctattttt tcagcaatag ctcttttctt
     5221 catgctcttc tgttttgccc accaaagttt aagcagcctt ccagtaggct tatactgcac
     5281 aaacttgtgg tttttctcat cctttgatac agcaacccct gcagttatca aagcgttgat
     5341 atagacaaga aatctccagt gctgccatct tcttatcctt ctgttaaaaa catctgcctt
     5401 gctaagttta tcataagctc tcgcaaggtc ttctggcttt tcatactcta atggcaggtt
     5461 ctgatcaagc caaagcatct ggtggtcaag atcctcagtg atattatcaa aagcagttat
     5521 ggcaactttt gggtctgttg tcttaaatat cttaaggagg gcactaggca tgctctcttc
     5581 ctggcttcgc tcaaaaagct cttcaatgct ttcctttgtt atctccctgc ctatctgagt
     5641 taaagtctga aggtcattga tagcagccct gcagtctccg ccagctcttc gtgcaagatt
     5701 tttgagtacc tctatatcac atctgatctt ctctttctcg catattcctc taaggatttc
     5761 agcgatctca gcataattta acccttccag ttcaataagg gtgcacttct ttcgcagagc
     5821 attgaacttg taatcccaag gatttgctga cgttagtatt atcggaaaag cagacctctc
     5881 aattatgtca gcaacagcag caagccctcc tctgtcttcc atccctgaaa ggccgtcaac
     5941 ttcatcaaca aggattatct tgccctttga aaataaagac atctgtaaag cagcgccacc
     6001 aagtttttgc cagatctctt ctttattcct ggtatcggac gcatttacct cgatgatttc
     6061 atacgaaagc tcactggcaa gagcatgcac aagagatgtc tttccgcagc ctgatggacc
     6121 ataaagccat atcgccttct tgccttttga gtaattcttg gcaagctccc tgatttcaga
     6181 gatagcttta tcctgccctt ttacatcggt gcagcattta ggatcatact ttttagtgaa
     6241 agggatcata gggaaaagat attatcagga atttattaaa gtattgattg aaaactgaca
     6301 caagcctaaa atttaaaaag tgtcaagaac cgccgggctg aaagcccggg gcgtgttctt
     6361 gagcatgctc agaaatcgct ggcttactct gggctaaagc atcagagcgt gcgcctgaag
     6421 cgatttctga catgtcaagt aatttagtca attatgctcg taaacatagt taaagcagat
     6481 attgaaacct tcaggctgca tcaaggcagg gttttggata tctatatgga aaccgcgaca
     6541 tcaggcgtga tgcagcagac cttaagcagg gatacagagt taaagtatat gctgggttta
     6601 tttgaggcag atggtcatgg ataccttgct tttgacggcg gcaatcttat tggatttatg
     6661 ctctccggtg cattaggtca cgatgatttg ttgcccggca cggttaggga aagattccct
     6721 gttgacagat gttattatat ctctgagatg catgtcgcaa aggagtacca aggcaaaggc
     6781 atcggttcac agatgatgca aaagtgtttg gatgagattg acaggcatag gtgggattac
     6841 ttgttcatcc gtgcctggaa ggaaaatgaa ggtgcgttaa agctctatca aaggtttgga
     6901 tttaaattgg acgagataat cgagcaggag aaaattaaaa aagacagaag cagtacgttt
     6961 ataatacaaa aacagtatct ttggaaaaag ttatagtata atctctggat taaaatcaga
     7021 ctaaggaagc aaattttata aaccgatcac caactattgc ttgttatgga actacctaag
     7081 ctttatgatc caaaggtttc agagccaaag tggcagaaat tttgggaaga aaatgatatt
     7141 taccgctttg atcaaaaaag cgataaggaa atattctcag tagacactcc ccctccgaca
     7201 gtctcaggaa agatgcacct aggccatgct ttctcatatt cccagcagga ctttgtggta
     7261 aggttccaaa ggatgcagca aaaaaacata ttctatcctt ttggaacaga tgataacggg
     7321 attcctacag agcgcctaat agaaaagaca aagaacgtca aagcttttca gatgaaaagg
     7381 caggagttca tagacctgtg tttagaaacg ctcgataaag agctcaggcc gaaatatatt
     7441 gccgattgga aaaggattgg catgtcctgc gactggaacc taaactacac gacgatcaac
     7501 aagcactgcc aaaagataag ccaaaaaagc tttatcgagc tgtatggcat ggacagagct
     7561 tacagaaaag aggctccggt catgtggtgt cctgattgtc agacaggaat ttcccaggtg
     7621 gaatgccagg ataaactatt agactcatac tttaatgata tagtcttcaa ggtagacgga
     7681 gaaaatctga taattgcaac aacaaggcca gagctgctcc ctgcatgtgt cgctgtattc
     7741 tttcaccctg acgacaagcg cta
//
LOCUS       DASRUK010000083         5730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_904101, whole genome shotgun sequence.
ACCESSION   DASRUK010000083 DASRUK010000000
VERSION     DASRUK010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904101"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2595
                     /locus_tag="VFF28_03695"
     CDS             <1..2595
                     /locus_tag="VFF28_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZX44763.1"
                     /translation="CPSCSTTLAKHELEYETVKDESIYLKFKVSGKKNEYLIIWTTTP
                     WTIAFNLGVMVNPDEEYVKVKVDNEVWVVGKKLANMFISSLDKKFEIISEMKGSELEG
                     VSYEHPFLSELKDWYSKLKKESPKVHTVLLSSEYVDMGSGSGLVHCAPGCGPEDYEVG
                     HKYGIIPFNRLDENGKYDNTMGKFTGWDAKKDNTKFIDELESKGALVARRPVEHEYAH
                     CWRCHGPVVFRTTKQWFFKVEDLKEKMRELNKKVKWVPDWAGSNWFDSWLDNLRDNGI
                     TRQRYWGTPLPIWKCKSCGEFVVVGSIAELKKLAGSVPSDLHRPHIDTVKIKCKCGGV
                     MERIPDILDVWIDAGTTSWTCLDYPQKKENFDKLWPPDFILEGKDQIRGWFNLLLVAS
                     MVSMGKHSYKACYMHGFVQDSSGRKMSKSLGNIISPYEVIDKFGADTLRYYMISGAAP
                     GLDVNYNMDDTKLKHKNLTVLWNVHKYLLDLASSVDVSKLQIDKKKCSIEEKYILSKL
                     NSTIKKVTSLYDEYRLNEVPWVIEELYLELSRTYIQMIRDKASIGSAEEKELVCAVIY
                     YVLFEALKLASPVMPFITEKMYLNLKEVFALKGDSIHMHIWPEYIEKDIDVSLEKNME
                     IMQGIVQSLLAAREKIGLGIRWPLKEVIIVSNDKRVVEAVEKLEDTIKNQTNIKEIRV
                     QESMPGIKLLVKPDFAKIGPDYGKLAAKIVARLAMESPETILSHIEKEGKFAMDVDGA
                     PVNVVKEYIIVTREVPAPYQEAEFKGGLGYINKEVTPELEAEGFSREIMRRVQNLRKN
                     AGLEKKDRIDLFILSPVDLSAHEEAIVEKVGANTLVLSESLPKAKFSVSGKEKIKGKE
                     FEIFFNKI"
     gene            complement(2592..3113)
                     /locus_tag="VFF28_03700"
     CDS             complement(2592..3113)
                     /locus_tag="VFF28_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44764.1"
                     /translation="MGHDQLIDRYLYALKYGDFQLIGELAAMAEYTDFVTPEELFGKE
                     ALSRISRLSRLGFPDIGQYWNGLIRIRQEEGATSYVAGGRLRAKTKLMKGRVHEDNDL
                     ALRLVNDAYYDPGLSSPIHWVGPAIVTVLLKLPIVYSRRAYLELTDPGHTRYGKAALK
                     ELIAEAYPTRLIS"
     gene            complement(3131..3289)
                     /locus_tag="VFF28_03705"
     CDS             complement(3131..3289)
                     /locus_tag="VFF28_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011507920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HZX44765.1"
                     /translation="MLGWALIFLIIAIIAAFFGFGGIAGLAATIAKVLFVIFLILFII
                     SLVRHFVR"
     gene            3468..4958
                     /locus_tag="VFF28_03710"
     CDS             3468..4958
                     /locus_tag="VFF28_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44766.1"
                     /translation="MMTKHSMQLYQEKPEQLMPLFLDRENIIGISSGFEGYTVILKRG
                     SVVVPNGLPVEADTSQKEAESGDVDDYQIPGRNQTKWDRRDRARDDIFHKSHGHKLRR
                     HSSSALDAILDFQDGQYEDTIPCRKKRIAETLAEYDEDGRLSRMLSGHREVKGHKTSL
                     RRHHLTVLDEMETDLDARVVPGRDEERRMYMLDINVGLIEGVKLRGRPYVYLIEGTFV
                     QSNLPVIAANGKPLQRHNPAKIHLKRGEYLFVAADSGRTFLGNQNIGVYSELNPVTLR
                     QLSDQYVHQKVRNGSRVQHGYKRKNVQIAWQVGVLLYQAAVGETPKYHVGDRDMQRTI
                     NFIQSIREQKGIEYALAYALNQSDGISKEAKRVLYDSILAMAVPKGNNGDSYTDKADL
                     ALAAIRRSNINPVDAAILIQQIEARSQKGMDKGYNRQVGDRSARMRPNLGPRSIYTTV
                     NIHPSPDSDGHYHSRELFDGQMAPTFGPITGQEHHIIKSRLKYQNR"
     gene            4984..5433
                     /locus_tag="VFF28_03715"
     CDS             4984..5433
                     /locus_tag="VFF28_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44767.1"
                     /translation="MPTISRDIPLAEITLRRYEKPDNLSGRELVRKLCLCLGLLQPGD
                     SRDVVVDVLNVVISQKKELSSEDIEKLVVDERKKNKLPMLGIAGSNIRRQLKRLRDIQ
                     ILEKVGNNYRINENASLTELFEEKIEKMLLPSITGRIKEYAKKLDEC"
BASE COUNT         1837 a         1018 c         1343 g         1532 t
ORIGIN      
        1 tgccccagct gttcgacaac ccttgcaaag catgagcttg aatatgagac tgtaaaggat
       61 gagtccatct atcttaagtt taaggtttct ggcaaaaaga atgaatatct gataatctgg
      121 acaacaactc cttggaccat agcttttaac cttggagtca tggtcaatcc cgacgaggag
      181 tacgtgaagg ttaaggttga taatgaggtt tgggttgttg gcaagaagct tgctaatatg
      241 ttcatttcct cactggataa gaagtttgag ataatttcag agatgaaagg gtctgaactc
      301 gaaggggttt cttatgaaca tccgttttta tctgagctta aagattggta tagcaagcta
      361 aagaaggaga gtcctaaggt ccatactgta ctgctttctt ctgaatatgt ggatatgggc
      421 tctggttcag ggcttgtgca ctgtgctcct ggctgcggcc ctgaggatta tgaggtcggg
      481 cataagtatg ggataattcc attcaatagg ttggatgaaa acggcaagta tgataatact
      541 atgggcaaat ttactggctg ggatgcaaag aaggataata ctaagttcat agatgagctg
      601 gaaagtaagg gagcgcttgt tgctcgcagg cctgtagagc atgagtatgc acactgctgg
      661 agatgccatg ggcctgttgt attcaggacg acaaagcagt ggttcttcaa ggttgaagac
      721 ctaaaagaga agatgcggga gctcaacaaa aaggtaaaat gggtgccaga ctgggctgga
      781 agcaactggt ttgattcctg gcttgataat cttagggata atggaataac aaggcagaga
      841 tactggggaa cccctcttcc tatttggaag tgcaaatctt gcggagagtt tgtggttgtg
      901 ggctctattg ctgagctaaa gaaacttgct ggaagcgttc cttcagatct gcataggcct
      961 catatcgaca cggtcaagat aaaatgcaag tgcggcggag ttatggagag gatccctgac
     1021 atcctcgatg tttggataga tgcagggaca acttcatgga cttgccttga ctatcctcaa
     1081 aaaaaagaga atttcgacaa attatggcct cctgatttca tacttgaggg aaaagatcag
     1141 atacgaggat ggttcaatct tttacttgtc gcctcgatgg tatcgatggg aaagcacagc
     1201 tacaaagctt gctatatgca tgggtttgtg caggactctt caggaagaaa gatgagcaaa
     1261 tcgcttggaa acataatctc tccgtatgag gttatcgaca agtttggagc tgacacacta
     1321 agatattata tgataagtgg agctgcacct ggactcgatg taaactataa catggacgac
     1381 actaaactga agcataagaa tttaactgtg ctttggaatg tgcataagta tctactagat
     1441 ctggcttcgt ctgtggatgt tagtaagctg caaattgata agaagaaatg ctcgattgaa
     1501 gagaaatata tcctctctaa gttgaactca actataaaga aagtgacatc cttatatgat
     1561 gagtataggt tgaatgaagt gccgtgggtc atcgaagagc tttatcttga gctatccagg
     1621 acctatatac agatgataag ggataaggct tctatcggca gcgccgaaga gaaagagttg
     1681 gtatgtgctg ttatctatta tgtgttgttc gaagctttga agcttgccag ccctgtcatg
     1741 ccgttcataa cagagaagat gtatctgaac cttaaggaag tgtttgcact gaagggagat
     1801 agcatccaca tgcacatatg gcctgagtat atcgagaaag atatcgatgt ttcgcttgag
     1861 aagaacatgg agataatgca gggaatagtt cagagcctgc ttgctgcacg tgagaagata
     1921 gggcttggaa taaggtggcc tttgaaggaa gttatcatag taagcaatga caaaagggtt
     1981 gtcgaggctg tagagaagct tgaagatact ataaagaatc aaacgaacat caaagagata
     2041 agagtgcagg agagcatgcc cgggataaag cttttggtaa aacctgactt tgcaaagata
     2101 gggcctgatt atggaaagct tgctgcaaag atagtcgctc gcttagctat ggaaagccct
     2161 gagacaatat tatcccatat agagaaagaa ggaaagtttg ccatggatgt ggacggcgca
     2221 cctgtaaacg tcgtcaaaga gtatatcatt gtcacacgtg aagtgcctgc tccttaccaa
     2281 gaggctgagt tcaaaggagg gctaggctat atcaacaagg aagtaactcc tgaacttgaa
     2341 gctgaagggt tttcacgaga gataatgcga agagtgcaga acctcaggaa gaatgcaggc
     2401 cttgaaaaga aggacaggat agacttgttc atactctcgc ctgttgacct tagcgctcat
     2461 gaagaggcta ttgtcgaaaa ggttggagca aatactcttg ttctttctga aagcctgcct
     2521 aaagcgaaat ttagcgttag cggcaaagag aagataaagg gaaaagagtt tgagatattc
     2581 tttaataaga tttaacttat aagtcttgtt ggataagcct cggcgatcaa ttccttgagt
     2641 gctgccttac cgtatcttgt gtggcctgga tcagtcagtt ctagatatgc cctccttgaa
     2701 tatacaatcg gtaactttaa caggacagta acaattgcag gaccgaccca atgtatgggt
     2761 gatgataggc caggatcata ataagcatcg ttgaccagcc ttagagccag gtcattatct
     2821 tcgtgcactc ttcccttcat taactttgtc tttgccctta gcctcccacc tgcaacatat
     2881 gaggttgcac cctcttcttg ccttatcctt atcagcccat tccaatactg ccctatatcg
     2941 ggaaaaccaa gccttgacag cctgctgatg cgactgagag cttctttgcc aaatagttct
     3001 tcaggcgtaa caaaatcagt atactctgcc atcgctgcca gttcaccaat gagctggaaa
     3061 tcaccgtact tcaatgcata aagatatcta tcaataagct gatcatgtcc cataaaatat
     3121 ttctgctgcc ttacctcacg aagtgccgga ccaaagagat gataaatagt atcaggaata
     3181 tcacaaacag cacttttgca atcgtcgctg caaggcctgc tatgcctcca aagccaaaaa
     3241 aagctgcgat tatcgctatt atcaagaata tcaatgccca tcctaacatc gccatagaaa
     3301 tacaccccct taccagacga taagctttta gggtttataa cgttttggtt gctttttcgt
     3361 cggtaattta tatagactat ataccgtaaa ctatctactg caacatagta atgtttataa
     3421 gggattagcg aatttactag ttgtcaaaat gataatgcgg gggatgaatg atgactaaac
     3481 actcgatgca attatatcaa gaaaaaccag aacaattaat gcctttattt ttagacaggg
     3541 aaaacataat tgggatttca agtggttttg aggggtatac agttatttta aaaagggggt
     3601 ctgtagtagt acccaatgga ctacctgttg aggcggatac ttcccaaaaa gaagctgaaa
     3661 gtggtgatgt agacgattat cagatacctg ggaggaatca aacaaaatgg gatcgaagag
     3721 atagagctag agacgatata tttcataaaa gtcatggcca taagctaaga agacattcat
     3781 cttctgcttt agatgctatc ctggatttcc aagatgggca atatgaagat actatcccct
     3841 gtagaaaaaa aaggatagca gagacgcttg ctgaatatga cgaagatgga agacttagtc
     3901 gaatgttaag tgggcacaga gaagttaaag gacataagac ttctttaaga cgacatcatc
     3961 ttactgtttt agatgaaatg gagactgacc tggatgcaag ggtagtccct ggcagagatg
     4021 aagaaaggag aatgtatatg cttgatatta acgtcggcct aattgaggga gttaaattga
     4081 ggggtcgacc ttatgtttat ctaattgaag gaacatttgt tcaaagcaac ctacccgtca
     4141 tagcagcaaa tggaaaaccg ttacagagac ataatcctgc aaaaatccat cttaaacgag
     4201 gggaatattt atttgttgct gcagatagtg gaaggacatt tttgggtaat cagaatattg
     4261 gagtttattc tgagcttaat cctgtcacct taaggcaatt atctgatcaa tatgttcatc
     4321 aaaaagttag gaatggaagt agagtccagc atggttacaa aagaaagaat gtgcagatag
     4381 catggcaagt gggtgtttta ttgtaccagg cagctgtagg tgaaactcct aaatatcatg
     4441 ttggggatag agatatgcaa agaacaatca attttataca atcaataaga gaacaaaaag
     4501 gaattgagta tgcacttgct tatgctttaa atcaatctga tggaatttct aaggaagcta
     4561 aaagagtatt gtatgattca atcttagcta tggccgtacc caagggtaat aatggtgaca
     4621 gctatactga caaagcagac cttgccctag cagcaataag gcgctcaaat atcaatccag
     4681 ttgatgcagc aattttaatt caacaaattg aagctaggtc tcaaaaaggc atggataaag
     4741 gatataatcg acaagtcgga gataggtcgg caaggatgag gcctaatctt gggcctagat
     4801 ctatttacac tacggtaaat attcatccaa gtccagatag cgatggtcac tatcattcaa
     4861 gagaactgtt tgatgggcaa atggctccga cttttggacc gataacagga caagagcacc
     4921 atataatcaa gtcaagacta aaataccaaa accgttaaat aatccttctt ttatttttac
     4981 tttatgccaa caataagcag agacatacct cttgctgaaa taaccctaag aaggtatgaa
     5041 aaacccgaca atctttccgg aagggagctt gtaagaaaac tctgcctttg ccttgggctt
     5101 ttgcaaccag gcgattctcg ggatgttgtc gtcgatgttc ttaacgttgt cataagccag
     5161 aaaaaagagc tttcttcaga ggatattgaa aaactggtcg tcgatgagag gaaaaagaac
     5221 aagcttccca tgcttggcat cgcaggctca aacatcagga ggcagctgaa gagacttaga
     5281 gatatccaaa ttctggagaa ggtgggaaac aattacagga taaatgagaa tgcttcctta
     5341 actgagctgt ttgaggaaaa gattgagaaa atgctattgc cttctattac tggaaggata
     5401 aaggaatatg ctaagaagct agatgaatgt tagttgccca gttgatatat gggttgatag
     5461 aatgaaacat tagtattccc tgggtcagaa attatcgaca ctggagctct aagaacctta
     5521 gcagcaagac cggtaaaata aagaccaaaa ccgtgttctc ccctgggagg gcatacagtt
     5581 aatcggtcta ttatagtaag ttaaatagaa aagttcttat ataagtaccc cttctgtaat
     5641 tagcaagagg tgatatagat ggcattaaga accaaatttc atcattcgtc aagtattttt
     5701 aagagactgt ataacaaaga aaacaaggtt
//
LOCUS       DASRUK010000084        11301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_578985, whole genome shotgun sequence.
ACCESSION   DASRUK010000084 DASRUK010000000
VERSION     DASRUK010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 11301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11301
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578985"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="VFF28_03720"
     CDS             <1..1194
                     /locus_tag="VFF28_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZX44768.1"
                     /translation="LIVVEHDEDTIRKADFVVDMGPGAGIDGGKIIAMGTPEEIERSK
                     ESLTGNYLSGKLRIETPKTRRKPDGHIRLKGCSEHNLKSIDIDIPTGVLTLITGVSGS
                     GKSTLIYEILYKAMMKKIYQSKEIPGKHKDIIFDSKTDKVIVIDQSPIGKTPRSNPAT
                     YTKLFDAIRDLFAETKEAKVRGYKPGRFSFNVSGGRCEACQGDGVIKIEMNFLPDVYV
                     ECEECKGKRYNKESLEVKYKDRSIADVLALSVDEALVLFENIPAIRKKLETLVRVGLG
                     YIKLGQSSTTLSGGEAQRIKLTRELSKNDSGQTLYLLDEPTTGLHFHDVKKLIDVLND
                     LVNKGNSVVVIEHNLDVIKSADHIIDLGPEGGEKGGMIVTTGTPEEVAGCSKSYTGMF
                     LKKLI"
     gene            1234..2196
                     /locus_tag="VFF28_03725"
     CDS             1234..2196
                     /locus_tag="VFF28_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44769.1"
                     /translation="MYVDVRQSNGRMRDGTFIDNLPPLVSVGRSPDLVGKVSHGLIDD
                     GADFLVLEGSKTVLRPDNLFDAYGKSLGLIEVDELKWASEPYSKDTLRVLFHSNPQSD
                     FYCHQPTRQGRMMHWFTDIDYYPIHGMVKIRKVPESKWNISYRLLVQANYSGAISHLI
                     QGPGDSIRFYCWSPKEPSLDMGMLSEALESPPGKIAYNFDRVPKGTKQLAEMIAGRLE
                     LGYDYSDKTVELSSPHAYGLITLTKDHIQFEPFVDPRDYYPTIEETIKEPTSKVTDPN
                     IALAFQLLMRTFKEQGVTLRVSGKQRRKFGSENCRYWTSAIVKL"
     gene            2259..3224
                     /locus_tag="VFF28_03730"
     CDS             2259..3224
                     /locus_tag="VFF28_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44770.1"
                     /translation="MALSRRDVLRGAALLGISGLDNEQPIRSNLEELTLDEYGCVVLP
                     QGDAVATNIWVPDLDIFRVNGIKYLFVDIGHMDRKTGIMTTPNYQIDSFLLRVAEFEN
                     RTGHRFILMPWNVVYASAQEPVSEFGSEAFRRNFISEYLALVGKGFDGIHLDVEAIPV
                     AKPGEIDQRALYLQMAADLRSELPKNKILSCYAPGVGDPRQNVYAWDKDYWTYMASIF
                     NLMTIQTYDTGQLTKEGFEAALSIQLDLVKSVNAALAATHHSSIMWPIPTHKPSPETM
                     QASIEWYNKSIGARAADYGIIGLFLFASWTMRPEHWAYYKASKAV"
     gene            3344..4054
                     /locus_tag="VFF28_03735"
     CDS             3344..4054
                     /locus_tag="VFF28_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44771.1"
                     /translation="MKKMFCLVLLSVLIAGCAKDYTTGSVVKAGDIESAVGSANDSRN
                     STTNYTNSDNTDMNNTISVLNQTLSPKVNKSINKTEFASNKARIEAIAKARARERELE
                     RKVRKEVEIEEKDNNANSENTPSNTSSNIMEEYDLKTYGNVSGAAQVSRIIGKAFVLK
                     TEAYFGSDTLVITDNADVSAGDYVKVKGTLTKLTAAQIEDIVGIKSDDYAGKYTVIAM
                     SIDEVSEEEATNTQLKFQ"
     gene            complement(4055..4432)
                     /locus_tag="VFF28_03740"
     CDS             complement(4055..4432)
                     /locus_tag="VFF28_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44772.1"
                     /translation="MGRFSRINLSLLRPSQDYLNQEGVNLYLKYIRKGKTLAPIPVAV
                     VDDSVVMLDCHHQCAAQSHITDKVDIWLADGPRDKIPSEYFAPQLRLRLTGINESIGR
                     RYHTVFSYDPGVQTIYDLVRGIR"
     gene            complement(4494..5936)
                     /locus_tag="VFF28_03745"
     CDS             complement(4494..5936)
                     /locus_tag="VFF28_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HZX44773.1"
                     /translation="MSIFKAYDVRGVYPTELNEELAYKIGRAFVSFLPVNKVVVSKDM
                     RESSESLKKELIRGITDQGADVIEIEGLCSTPRNYFACWHLKAPASIMVTASHNPGKY
                     NGFKFTREEAIPISGDTGIKEIERLVDLDMSEIASGARSDALAQSKPAISEHAQEHAP
                     TFRSAVLDRFANPKKKGKVTKENIEEQYKKHVLSLIDADKIRPLKVVIDAGNGMGGRD
                     LELVLGDLPLKVVKMYFEPDGSFPNHEANPLKEENIADLKKRVVAEKADFGIALDGDA
                     DRVFFVDEKGETVAADFITSLIAEEILKEHKGSKILYDLRSSWIVKENVEKNGGKSLM
                     CRVGHAFIKEQMRQEKAAFAGELSGHFYFKDNFYTDSGIVAALKMIQIISEKGMKLSE
                     LIAPLNKYHCSGEINSEVEDKDAKMKELADIYKDGSVSWLDGIRVDFDDWWFNVRASN
                     TEPLLRLNLEAKTKRLMEEKRDEILKVIRS"
     gene            6116..6838
                     /locus_tag="VFF28_03750"
     CDS             6116..6838
                     /locus_tag="VFF28_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44774.1"
                     /translation="MANRPFHLSHSTDHSLCIGLYDILRFGAFDPKKANAVLEHRIYE
                     LDSPYPKFSANHPQEICMVNGQAVHVCDGLQAEYVAYSPKEILVQYTTDRQLSKLFPN
                     EQPWRPAFKKDGILEENFNNPLRCLVVKKKHMYSLSLAVEEGDGHIYFLADNGVGFHL
                     IMDIYQGAIQPPRNDLPLIFQYFSNLRDPDRQVAWLRQQQPTMEKFERIPVDYSRNKL
                     VKALIRGLSAAGQLATPLHPQQ"
     gene            complement(6813..7676)
                     /locus_tag="VFF28_03755"
     CDS             complement(6813..7676)
                     /locus_tag="VFF28_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZX44775.1"
                     /translation="MKERITITLNEELIEQLDKRVDGEQIKNRSQEIEKILEDALGVS
                     IPEKALILAGGKGTRLRPLTLQTPKALIDVQGKTLTEWLFDLLKKYGIRDVVLSVGYL
                     SDKIKDYFTDGSKFGMNVDYVVEDPENPLGTAGPLKLAQHMLKDSFIVSNGDELKSIN
                     IPRMYRLHKRKKALATIALTSVNNPSQYGVARLDGSRIVEFVEKPKKEDAPSNLINSG
                     FYIIEPEVIDMIPSGKFCMLEKDIFPKLAKEGRLRGFPFSGQWFDTGNMERLEITRKQ
                     WKGIIVEDEGE"
     gene            7791..8849
                     /locus_tag="VFF28_03760"
     CDS             7791..8849
                     /locus_tag="VFF28_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HZX44776.1"
                     /translation="MAITGSKKKQIAVLTGGGDAPGLNAVIRGVVVNSKKLGYDVLGI
                     LDGWRGLLDKKHAGTLDLDSVEDIHMLGGTILHTSRTNPYKVENGPSRIKENLKALNC
                     DYLIAIGGEDTLGVANKLAKEGVKVVGVPKTIDNDLWGTDYTFGFNTAITRATEAIEN
                     LHTTAKSHHRTILVEVMGRHAGWMTLEAGIGGGASIILLPEEPFDLDQITKILLNRKR
                     KGKNYAIIAVSEGAVPKEGQMILQAKEKDSFGHVRLGGIAEILGKEIEKRTDIECRHV
                     VLGHLQRAGDPTVFDRVLGTRLGVKAAEMIDKGKFGMMAALRGTDIVAIPLEEAVGKL
                     KTVPKERYDEAKLFFETD"
     gene            8862..9980
                     /locus_tag="VFF28_03765"
     CDS             8862..9980
                     /locus_tag="VFF28_03765"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HZX44777.1"
                     /translation="MKAVPGNLVYDALKDEQAIILACNPRITIGVAEGVFKAAKDLDS
                     PVIMELARSECNQNIGYTGLTPQTLSANLNKADEKIGHDLWILHADHIQVRKGTPDEI
                     VEIKKLIDAQINAGYSSFAIDASHLFNFQGKTVEEELAPNLNVTIEIAKYIKTRLPSF
                     GLEVEVGEIGRKDSCGMVLTKPEEAVAYIKSLVKAGINPNLIAIANGSTHGNCYDKDG
                     NMIAQTTIDIEQTKKVAQALRDNDCHVRIAQHGITGTPLDIIKNKFPHGDILKGNVAT
                     LFQNIVYDTIQKYHTSLWNEIYEWVLKNHPIEGKKPNEVFGKNVKYATKEFFARIYDM
                     SDECRQEIEEKAYEAASDHIKAFNAEGTASIVRKKLNR"
     gene            9985..11016
                     /locus_tag="VFF28_03770"
     CDS             9985..11016
                     /locus_tag="VFF28_03770"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014122186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HZX44778.1"
                     /translation="MADKIRVGIVGYGTIGKRVADAVMLQDDMELSGITAHSYNYRMI
                     AAGLKSIPMYKFPGAEDLDGLKIAGDFDALLNDVDVIVDCSPKKGGAENKEKYYIPKK
                     IKAIFQGGEKAKVADASFTAQVNYKKAVGKNFVRVVSCNTTGLTRTLNAVKGRYGIDA
                     VHATMIRRGADPWDIKHGPINAIVPVLELPSHHGPDVQTVMEGLPIFTTALSIPTTLM
                     HLHSVTVDCKETPDVKEILDLFQKTTRVRVLDNASGVRSTAEIMEFAKELSRDRCDMP
                     EIVVWKEAVGVTGNKLLYLQAIHQESDVVCENVDAIRAAMGFDDAEESIRKTNTALKI
                     DYCKELCKK"
     gene            complement(11044..>11301)
                     /locus_tag="VFF28_03775"
     CDS             complement(11044..>11301)
                     /locus_tag="VFF28_03775"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HZX44779.1"
                     /translation="DGGIAKNRTRLLTEIFANIARMNNERWEGWQGEILIDEKGKDDT
                     WVGRNAYYKPVIVRGDVKQGDFVKVRIGNVTSYDLRGDIIY"
BASE COUNT         3544 a         2337 c         2452 g         2968 t
ORIGIN      
        1 ctgatcgtcg tcgaacatga tgaagacacc ataagaaaag ctgattttgt cgttgacatg
       61 ggcccaggcg caggcattga tggtgggaag ataatcgcaa tgggaacacc tgaagagata
      121 gagcgctcaa aagaatcatt aactggaaac tacttgtctg gcaagttaag gattgagacg
      181 ccaaagacaa gaagaaaacc agatggccat atacgcctca aaggctgctc tgagcataac
      241 cttaagtcta tagacataga cattccgact ggtgtcttga cattgatcac cggagtaagc
      301 ggcagcggga aatcaacgct gatttatgaa attttataca aagccatgat gaaaaagatt
      361 taccagtcaa aagagatccc aggcaagcac aaggatataa tctttgactc aaaaactgat
      421 aaagttatag tcatcgacca aagccctatc ggaaagacgc ccaggtcaaa ccctgcaaca
      481 tatacaaagc tatttgatgc gataagggac ctgtttgcag agacaaaaga agcaaaggta
      541 aggggctaca agccaggaag gttctcattc aatgtttcag gcggaagatg tgaagcatgc
      601 caaggtgacg gagtgataaa gatagagatg aatttccttc cggatgttta tgttgaatgc
      661 gaggaatgca aaggaaagag atacaataaa gagagccttg aagtaaaata caaggataga
      721 tccatagccg acgttcttgc actaagtgta gatgaagccc tcgtcctttt tgaaaacatc
      781 cctgccataa gaaagaaact ggaaacactt gtcagagtcg ggcttggata tatcaagctc
      841 ggccaaagtt caacaaccct gtcaggcggt gaagctcaaa ggataaaact cacaagagag
      901 ctttcaaaga acgacagtgg ccagacacta tatctgcttg atgagccgac aacaggcctc
      961 cattttcatg atgtaaaaaa gcttatagac gtattaaacg accttgtaaa caaaggcaac
     1021 agtgttgttg tgatagagca caatcttgat gtgataaagt ctgcagacca tatcatcgac
     1081 cttggccctg aaggtggaga gaaaggtggg atgatagtga cgacaggaac gccggaagaa
     1141 gttgcaggat gttctaagag ttatactggg atgtttctaa aaaaattgat ttgaccatta
     1201 tctttttaag gcaagcggta tctctctaag agtatgtatg tggacgttag acaatcaaat
     1261 ggtagaatga gagatggcac ttttattgat aatttgcctc cattagtaag cgttggaagg
     1321 tcgcccgatc tagttggcaa ggtttctcat ggattgattg atgacggagc tgatttttta
     1381 gttcttgaag gctctaaaac ggtattacgg ccagacaatt tatttgacgc atacggaaaa
     1441 tctctgggtc taattgaagt agatgagtta aaatgggcat ctgaaccgta ttcaaaagac
     1501 actttgcgag tattgtttca ctcaaacccg caatctgatt tttattgcca tcaaccaact
     1561 cgtcaaggaa ggatgatgca ttggtttact gatattgatt attacccaat acatggcatg
     1621 gtaaagataa gaaaggtgcc ggaatcaaag tggaatatct cctaccgtct cttggttcag
     1681 gcaaattata gtggagcaat aagtcacttg atccaaggcc caggagattc tatcagattc
     1741 tattgctggt ctccaaaaga gccaagcctg gatatgggaa tgctttctga agcgttggaa
     1801 tctccccctg ggaaaatagc atataacttt gatagagtgc caaaaggaac aaaacagttg
     1861 gcagagatga ttgcaggtag gttagaatta gggtatgatt attcagataa aaccgtggag
     1921 ttgtcaagcc cacatgcata tggcttgatt acactaacta aggatcatat acaatttgaa
     1981 ccgtttgttg atcccagaga ctactatcct acgatagaag agacaattaa agaaccaaca
     2041 tctaaagtga ctgatccaaa catagccctg gctttccagc tattgatgag gacatttaag
     2101 gaacaagggg taacccttag ggtgagcgga aaacaacgta gaaagtttgg atctgaaaat
     2161 tgcagatatt ggacaagtgc tatcgtcaaa ttatgattgc cagcgctaac taactcttct
     2221 cagcgagact ttttaaccct ccaaactata gctagtatat ggctttatcc agaagagatg
     2281 tattgagggg ggcagcgctg cttggtataa gcgggttgga caacgaacaa cctatccgct
     2341 ctaaccttga agagctcaca ttggatgagt atggctgcgt tgttctgccc cagggagatg
     2401 cagttgcaac caacatatgg gtgcctgatc ttgatatttt tagggttaat ggaataaaat
     2461 atctctttgt agatattgga catatggata ggaagactgg cataatgaca acacctaatt
     2521 atcagataga ttcttttctt ttgagagttg cagagtttga aaacagaact ggtcaccgat
     2581 ttatcttgat gccatggaac gtagtgtatg cctcagcaca ggagccggta tctgaatttg
     2641 gatcggaggc tttcaggaga aattttatca gtgaatatct agcgcttgtt ggaaaaggat
     2701 ttgatggaat acaccttgat gttgaggcaa tacctgttgc caaaccagga gagatagacc
     2761 agcgggcatt gtacctgcag atggcagctg atcttagaag tgaacttcca aaaaacaaaa
     2821 tcctttcttg ctatgcacca ggtgttgggg atcccaggca gaatgtctat gcatgggata
     2881 aagattattg gacctatatg gcaagtatat tcaaccttat gactatccaa acatatgata
     2941 ctggccaatt gacaaaggaa ggatttgaag ctgccctgtc aatccagctt gatctggtca
     3001 agagtgtaaa tgcagcactt gctgcaactc atcacagcag cataatgtgg cctatcccca
     3061 cgcacaagcc aagccctgaa accatgcagg catccataga atggtacaat aaatccatcg
     3121 gcgcaagggc tgcagattat ggcataatcg ggctctttct ctttgcaagt tggacgatga
     3181 ggcctgagca ttgggcttat tataaagcgt cgaaagctgt ttaactcatc agcctatatc
     3241 ctcaatttta ctgctcctat cgtttagtta gtgatgatat agccctaaac ccctcagcta
     3301 acctttttat tagttttgct tgttctaaat tagcggtgat tttatgaaaa aaatgttttg
     3361 tttggtgtta ttatcagttt tgattgccgg ctgtgcaaaa gattatacaa ctggaagtgt
     3421 ggtaaaagca ggcgatatag aatctgcagt gggtagcgca aatgattcaa ggaatagtac
     3481 gacaaattat acaaatagcg ataatactga tatgaataac actataagcg tccttaacca
     3541 aacacttagt ccaaaggtca acaaatcaat aaataaaact gagtttgcaa gcaacaaagc
     3601 aaggattgag gcgatagcca aggcaagagc aagagaaaga gagcttgaga gaaaggtaag
     3661 gaaagaagtt gaaatagagg aaaaggacaa taatgcaaac agtgaaaata ccccctctaa
     3721 cacatcatca aatattatgg aggaatatga cctcaaaacc tacggaaatg tatccggagc
     3781 agcgcaggtc agcaggatta ttggcaaagc atttgtctta aaaacagaag catattttgg
     3841 cagcgacact ttggtgataa cagacaacgc tgatgtatca gccggagatt atgtcaaagt
     3901 aaaaggaaca ttgacaaaac tcaccgcagc ccagatagaa gatattgtag gcattaaatc
     3961 agatgactac gctggaaaat acacggtcat cgccatgtcc atagatgaag ttagcgaaga
     4021 agaggcgaca aatacacagt taaaatttca atgattatct gattccgcgg actaagtcat
     4081 atattgtttg tacgcccgga tcataactaa agacagtatg atatctcctg cctatacttt
     4141 cattaatgcc agttaacctc agcctcagtt gaggagcaaa atattctgat gggattttat
     4201 ctcttggacc atcagccaac catatatcca ccttatcagt gatatgactt tgtgcagcac
     4261 attggtgatg gcaatctagc atgacgactg aatcatcaac gactgccaca ggtatcggag
     4321 ctaatgtttt gcctttccta atatatttaa ggtataaatt aacaccctcc tgatttagat
     4381 agtcctgact aggcctaagc agactaaggt ttatcctgct aaaccgtccc ataatttaag
     4441 aagttcaacc cttgtttata aaggttatta tttactatta aacagtaata actctatgac
     4501 cgaataactt tcagaatctc atcccttttt tcttccatca atctctttgt tttcgcttca
     4561 aggttaagcc taagcagcgg ctctgtgttt gacgcacgca cattaaacca ccaatcatca
     4621 aaatcaaccc tgattccatc aagccatgat acagagccat ctttatagat gtcagcaagc
     4681 tctttcatct tagcgtcctt atcctccact tctgagttta tctctccaga gcaatgatat
     4741 ttattcaaag gagctataag ttcacttagc ttcatgccct tctcagagat tatctgtatc
     4801 atcttgagcg cagcaactat tcctgaatca gtgtaaaaat tatccttaaa gtaaaaatgc
     4861 ccggaaagtt ctcctgcaaa agcagccttt tcctgcctca tctgttcttt tatgaatgca
     4921 tgccccaccc ggcacataag ggattttcct ccatttttct ccacattttc cttcactatc
     4981 catgaactcc tgaggtcata aagtatcttg ctgcctttgt gttccttcag tatttcctct
     5041 gcaatcaatg aagtgataaa atcagcagca accgtctctc ctttctcatc cacaaaaaat
     5101 actctgtctg catcgccatc aagggcaatc ccaaaatcag ctttctcagc aacaactctt
     5161 ttttttaggt cagcgatgtt ctcctccttc aagggatttg cctcatggtt tggaaaactt
     5221 ccatcaggct caaaatacat cttcacaact tttaaaggca agtcaccaag aactaattcc
     5281 aagtccctgc cacccatgcc gttgcctgca tcgatgacaa cctttagcgg ccttattttg
     5341 tcagcatcga tcaagcttag aacatgcttt ttgtattgct cttcaatatt ctccttggtt
     5401 accttccctt ttttcttggg attggcaaac ctgtcaagaa ccgccgacct aaaggtcggg
     5461 gcgtgttctt gagcatgctc agaaatcgct ggcttacttt gggctaaagc atcagagcgt
     5521 gcgcccgaag cgatttctga catgtccaga tctaccaagc gctctatctc ttttatccct
     5581 gtatcgcccg agataggtat agcctcttcc cgtgtaaatt taaagccatt gtatttgcca
     5641 ggattatgtg aagcagtgac cattatcgaa gcaggagctt taaggtgcca gcatgcaaaa
     5701 taatttcttg gagtgctgca aagcccttct atctctatta catcagcacc ttggtcggtt
     5761 atccctctta tcaattcctt tttgagggac tcagaactct ccctcatatc tttggacaca
     5821 acaaccttat tgacaggcag aaagctgaca aaagcccttc ctatcttata ggcaagctcc
     5881 tcattcagct cagtgggata tacccctctg acatcatagg ccttgaagat agacatctgg
     5941 tgataaatgg acagagattt tataaatttt gttaatgatt gctcaaactg agtacatcaa
     6001 cagcttgcta cgattcaggt ggaggcttaa aaaccctgca gctcggctag cgtttggaag
     6061 gttattggtt aaaaaatcgc aagtataata aaaagacttt agattttctt aagtgatggc
     6121 aaaccgaccg ttccatttat cccatagcac ggaccatagc ctatgcatcg gattatatga
     6181 catacttagg ttcggcgcat tcgatccaaa aaaggcaaat gctgttcttg agcatagaat
     6241 ctatgaattg gatagcccat atcccaaatt tagtgcaaac catccacagg aaatatgtat
     6301 ggtcaacgga caagcggtgc atgtttgtga tggtcttcag gctgagtatg ttgcttatag
     6361 cccaaaagaa atccttgtac aatataccac cgaccgccag ctatctaagc tttttcctaa
     6421 tgagcagcct tggaggcctg catttaagaa agatggtata cttgaggaga attttaataa
     6481 tcccttaagg tgcctggtag taaaaaagaa acatatgtac agtttatcct tagcggtgga
     6541 ggaaggagat gggcatatct atttcttggc cgataatgga gttggttttc accttatcat
     6601 ggacatctat caaggagcaa tccaacctcc acgaaacgat ctgcccctta tttttcaata
     6661 tttctctaat ctgcgagatc ctgaccgaca ggttgcttgg ctaaggcagc aacaacctac
     6721 catggagaaa tttgagagga ttccggttga ctacagcaga aataaattag taaaagctct
     6781 gataaggggg ttatctgctg caggacagct agctactccc cttcatcctc aacaataatg
     6841 cctttccatt gcttcctggt tatctcaagt ctttccatat tgcctgtgtc aaaccattgc
     6901 ccagaaaaag gaaatccacg cagccttcct tccttggcga gctttggaaa gatatccttc
     6961 tcaagcatgc agaatttccc agaaggtatc atatcgatca cttcaggctc gattatatag
     7021 aaacctgaat ttatcaggtt ggaaggggca tcttccttct ttggcttttc gacaaactca
     7081 actatccttg atccgtccaa gcgagcaact ccatactgcg aaggattatt cacagatgtc
     7141 aaagcaatcg tagcaagcgc tttctttctc ttgtgcaggc gatacatccg cggaatattg
     7201 atagacttta gctcatcacc atttgagacg ataaatgaat ctttaagcat gtgctgtgca
     7261 agctttagag gccctgctgt cccaagcgga ttctcaggat cttcaacaac atagtcaaca
     7321 ttcatcccaa atttgcttcc atcggtaaaa taatctttta tcttgtcaga tagataaccg
     7381 acagacaata caacatcccg aattccatat tttttaagca gatcaaacaa ccattcagta
     7441 agggtttttc cttgcacatc aataagtgcc tttggagttt gaagagtaag aggcctaagc
     7501 ctcgttcctt tgcctccagc aagtatcaaa gctttctctg gtatagacac gccaagtgca
     7561 tcttcgagta tcttctctat ctcctggctt ctgtttttta tctgctcgcc gtccactctc
     7621 ttgtctagct gttcaataag ctcttcattg agggtgatgg ttatccgttc cttcatgcaa
     7681 atagcccaag tatctagtat ttataaagat tacgcttttc gtatgataat gtatgataat
     7741 cgaaaacttt aaatattagt gagattttag ccgctagtta gggtggctac atggcaataa
     7801 caggctcaaa gaaaaaacag atagctgtac tcacaggcgg cggagatgcg ccaggcctca
     7861 atgcagtaat caggggagta gtagtcaact ctaaaaagtt aggatatgat gttcttggga
     7921 ttttagatgg atggagaggt ttgcttgaca aaaagcacgc aggcacattg gaccttgaca
     7981 gcgtagaaga tatacacatg ctgggcggca ctatccttca cacttcaaga actaacccct
     8041 acaaggttga aaacggccca agcaggataa aagaaaactt aaaggctttg aactgcgact
     8101 acctcatagc aataggtggc gaagatacct taggcgttgc aaacaagctt gcaaaggagg
     8161 gtgtaaaggt agtcggcgtt ccaaaaacca ttgataatga cctatggggt actgattata
     8221 cattcggctt caacacagca ataacaagag cgacagaagc gattgaaaat ctccatacaa
     8281 cagcaaaatc gcaccatagg actatcctgg tggaagttat gggaaggcat gctggctgga
     8341 tgacgctaga ggcaggtata ggcggcggtg cttcgataat actcttgcca gaagagccat
     8401 ttgaccttga ccaaattaca aagatcctgc tcaacagaaa aaggaaagga aagaattatg
     8461 ctatcatcgc agtatctgaa ggcgctgtcc caaaagaagg ccagatgatc ttgcaggcaa
     8521 aggaaaaaga ttcttttggc catgtcaggc tgggaggcat agctgagata cttggaaaag
     8581 agatagaaaa aaggacagat atagagtgca ggcatgttgt acttgggcat ctccaaaggg
     8641 caggggatcc tactgtcttt gaccgggttc ttggaacaag gcttggagta aaagcagcag
     8701 agatgataga taagggcaag tttgggatga tggctgcgct gagagggact gacatagtgg
     8761 cgattccgct tgaagaggca gtaggcaagc tcaagaccgt tccaaaagaa cgttatgatg
     8821 aagctaagct tttctttgaa acagattagg aaggtgatat tatgaaagca gtaccaggta
     8881 atcttgtata cgatgctcta aaagacgagc aggcgataat tctagcatgc aatccgagga
     8941 tcacgatagg cgttgcagag ggtgtcttca aagcagcaaa agatcttgat tcacccgtca
     9001 tcatggagct tgcaagatca gaatgcaacc aaaatatcgg ctatacaggc cttacaccgc
     9061 agactctttc ggctaacctt aacaaagcag atgaaaagat aggccatgac ctatggatac
     9121 ttcatgcaga tcacatccag gtaagaaaag ggacgcctga tgagattgtg gagataaaaa
     9181 agctcatcga tgcccagata aacgcaggat attccagctt tgcgatagat gcatcacacc
     9241 tcttcaactt ccagggaaag acagttgaag aagagcttgc tccaaatctc aatgtaacta
     9301 tagagattgc aaagtatatc aagaccaggc tgccaagctt cggtttagaa gttgaggttg
     9361 gagagatagg cagaaaagac agctgcggca tggtgctgac aaagccagaa gaagcagtcg
     9421 catatatcaa aagcctggtc aaagcaggga tcaatcctaa tcttatagcc atcgcaaatg
     9481 gctcaacgca cggcaattgt tatgataagg atggcaatat gatagctcag actactatcg
     9541 acatagaaca gacaaagaaa gttgcacaag cgcttaggga taacgattgt cacgtaagga
     9601 tagcacagca tggcatcaca ggaacacccc tcgacataat aaagaacaag tttcctcacg
     9661 gagatatttt gaagggaaat gttgcaaccc tgttccaaaa tatagtctat gacaccatcc
     9721 aaaaatatca tacaagccta tggaatgaga tttatgaatg ggtactaaag aatcatccga
     9781 tagagggcaa aaagccaaat gaagtctttg gaaagaacgt caagtatgct acaaaggaat
     9841 tctttgcaag gatatatgat atgtcagacg aatgcaggca ggagatagag gaaaaagcct
     9901 atgaagcagc ttctgaccac atcaaagcct tcaatgcaga agggacagca tcgatagtaa
     9961 gaaaaaaact caacaggtga taacatggct gataagataa gggtagggat tgttggttac
    10021 ggcactattg gaaagcgtgt tgcagacgct gtcatgcttc aggatgatat ggagctttca
    10081 ggtatcactg cccacagcta taactatagg atgatagctg ccgggctcaa gagcatccct
    10141 atgtataagt ttcctggagc agaggactta gatggcctaa agatcgcagg agactttgat
    10201 gcactattaa acgatgtaga tgttatcgtc gattgctctc caaagaaagg cggcgcagaa
    10261 aacaaggaaa aatactacat cccaaaaaag ataaaggcga tcttccaggg aggagaaaaa
    10321 gcaaaagttg cagatgccag ttttacagcc caggtaaact acaaaaaggc agtaggcaaa
    10381 aactttgtca gagttgttag ctgcaatacc acaggcctga caaggacgct taatgcagtt
    10441 aaaggaagat atggaataga tgctgttcac gcgactatga taagaagagg cgcagacccc
    10501 tgggacataa agcatggccc gataaatgcc atcgttcccg tgcttgagct tccttcacac
    10561 cacggtcctg atgttcagac agtcatggaa ggcctcccta tatttacaac agcattgtct
    10621 attccaacaa cactgatgca tctgcattca gttacagtag actgcaaaga aacacctgat
    10681 gtcaaagaga tacttgactt gttccaaaag acgacaagag taagggtgct tgacaatgcc
    10741 tcaggagtga ggtcaacagc agagataatg gagtttgcaa aggaacttag cagggaccgc
    10801 tgtgacatgc cagagatagt tgtctggaaa gaagcagtgg gagttacagg aaacaagctc
    10861 ctgtacctgc aagcaatcca ccaggaatct gatgttgtct gtgagaatgt tgatgcgata
    10921 agggcagcta tgggatttga tgacgctgaa gagtcgataa gaaagacaaa cacggcattg
    10981 aagatcgact actgtaaaga actatgtaaa aaataatttt tttatatcta attaaagaag
    11041 taatcagtat attatatctc ccctaagatc ataagaagtg acattaccta tcctcacctt
    11101 gacaaaatct ccttgcttga catcccctct gacaatcact ggcttatagt aagcgttcct
    11161 gccaacccac gtatcgtctt ttcctttctc atctataagt atctctccct gccagccctc
    11221 ccacctctcg ttgttcatcc tggcgatatt tgcaaatatc tctgtaagta accttgtcct
    11281 gttctttgca atacctccat c
//
LOCUS       DASRUK010000085         3836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_904184, whole genome shotgun sequence.
ACCESSION   DASRUK010000085 DASRUK010000000
VERSION     DASRUK010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904184"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..252)
                     /locus_tag="VFF28_03780"
                     /pseudo
     CDS             complement(139..252)
                     /locus_tag="VFF28_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020621211.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
     gene            complement(255..1148)
                     /gene="epsC"
                     /locus_tag="VFF28_03785"
     CDS             complement(255..1148)
                     /gene="epsC"
                     /locus_tag="VFF28_03785"
                     /inference="COORDINATES: protein motif:HMM:NF041874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HZX44780.1"
                     /translation="MKKYEYNGEAIKGLFASYKSDSPPFRTTAATFPERNSEWAEVSA
                     LEQLMFPYYHNKGKATRNGRALQRQLSKFGQLLYSSIIPYVGDDESLPVGSDVSGIVT
                     KVIDQLPSVREKLKSDVSAAFEKDPASNNFGEIIRAYPGFKAVQVHRLAHELYRQGAK
                     SAAREYSEHAHSWAGIDIHPGAQIGYGFFIDHGTKVIIGETTKIGNGVKIYGGVLLGA
                     KSNGKGRADKGKKRHPTIGDGTVIYTDVRVLGDINIGKNCIIGANCTITEDIKPDMKV
                     VINDDGEQIIEPRNKQLAGVR"
     gene            complement(1411..2649)
                     /locus_tag="VFF28_03790"
     CDS             complement(1411..2649)
                     /locus_tag="VFF28_03790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HZX44781.1"
                     /translation="MIVQRDNIGLFGRVNVGKSTIMNLLTQQECSIVDVKPGTTADIK
                     SMPAEIHGLGPVNLFDTAGIDEDGVLGEKKRTKTFNCLRECDLALLAVNPSSEDFAWE
                     EMIMSEAKKLGKQLLVIFNVFDEDSRHGLFNSYKSIGIKAIDTSCRQPLLEFILNNFK
                     PGNKQFELLPFIEEGQFYVLNIPMDEETPQGRLLRPQQMVEEYITRRWAYPVSYRMNL
                     SKARKGDADEKNRFEEFIQRIKPKAIITDSQAMDIMAKWVFDDIMLTTFSIVMINHMS
                     RGRLDTFVEGVKALDTLKIGDKVLIAEACNHSRISEDIGTVQIPRYLKEYYPGVIVEH
                     SFGREFPANLKEYKLIIHCGGCMIDSQKLGARLEELGKAGVSITNYGIFLAAINGKEA
                     LDRVLRPWRLKSSLALGKNV"
     gene            complement(2646..3710)
                     /gene="hydE"
                     /locus_tag="VFF28_03795"
     CDS             complement(2646..3710)
                     /gene="hydE"
                     /locus_tag="VFF28_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HZX44782.1"
                     /translation="MENETYISKMMRVLHKEDLSKEDIIFLLSLERKDELESLYQYAD
                     RVRKEHLSDDVHIRGILEFSNHCKNDCLYCGIRRSNLNLDRYRLDKAQIIDLAKNAVD
                     KLGFKTVLLQSGEDDCYTADMIEEIIRGILNECDCRVALSIGERSIEDYKRFFEAGAR
                     RCLVRFETSNEKLYGSLHPNSSMTKNSFKERIDLIKKFKQIGYQVGTGSMIGLPGQTI
                     EDMADDILLAKQLGADMVGMGPFLSHHDTPLFDCANGDFEMVLKMIAVTRLVCKDVFI
                     PATTALQTLNLANGRQLALGCGANLLMPNITPGKYRRYYQLYPNKACIYEEPDDCSGC
                     VKRLILSVKRTIGTGYGDRA"
BASE COUNT          973 a          928 c          655 g         1280 t
ORIGIN      
        1 aaccttgttt tctttgttat acagtctctt aaaaatactt gacgaatgat gaaatttggt
       61 tcttaatgcc atctatatca cctcttgcta attacagaag gggtacttat ataagaactt
      121 ttctatttaa cttactataa agctctcaag ctttgcatat attgtcgcag agcccttatc
      181 gattatcttg tttaacttga ctacaggtgt atcaccgatc aattctaata tgttgtttgc
      241 tacctttggc atttttacct cacccctgct aattgtttgt ttcttggctc aattatctgc
      301 tcgccatcat cattgataac taccttcata tccggcttga tatcttctgt gattgtgcag
      361 tttgccccga tgatgcaatt cttcccaatg ttgatgtcgc ctagtactct gacatcggta
      421 tatatcaccg tgccatctcc aatagtaggg tgtcttttct tgcctttgtc agcccttcct
      481 tttccattgc ttttggcgcc aagcaataca ccgccataga tttttacgcc gttccctatt
      541 ttagttgttt cgcctatgat aaccttagtc ccatggtcta taaagaatcc gtagcctatc
      601 tgtgctcctg gatggatatc tattcctgcc caggaatggg catgttcgct atactctctt
      661 gcagcagatt tagctccttg cctatatagc tcgtgagcaa gtcggtggac ctggactgct
      721 ttaaaaccag gataagctct gattatctcg ccaaaattgt ttgaagcagg atctttttca
      781 aaagcagcac taacatctga tttaagcttc tctcgtacag agggcagttg atctataact
      841 ttggtcacta tgccagaaac atctgacccg actggcaagc tctcatcatc tcccacatat
      901 ggaattatgc ttgaatacaa cagctgcccg aatttactta gctgcctttg cagggctctt
      961 ccattccttg ttgctttgcc cttgttatga tagtatggga acatcaattg ttctaaggcg
     1021 cttacttcgg cccattcact gtttctttca gggaatgttg cggcagttgt tctaaatgga
     1081 gggctgtctg acttgtagga tgcaaacagt ccctttattg cctctccatt gtattcatac
     1141 tttttcattt ttccttgaac tcctcaaatt tatctatcga atcatttatt atgaaaatca
     1201 ttatcatcac ctcgcatgaa aaaaattata taattagtta tagtttgatt ttagccttgg
     1261 acaatgagcg ctgtctttca acaacagctg cagctacagc aagaagaaat attcacttta
     1321 tttagtttaa aaccactcat tagagttaac aattgtaaac aagtatttaa atctttctaa
     1381 aaagcggttg gttaagacga taaactctat ctaaacgttc ttgcctagtg ccaggctgct
     1441 tttcagcctc caaggcctaa gtaccctatc aagagcttct ttcccattga ttgcagcaag
     1501 aaatatccca tagtttgtta tcgaaacacc agctttccca agctcctcca gccttgcacc
     1561 aagcttctgg ctgtctatca tgcagcctcc gcaatgtatt atcaatttat attctttaag
     1621 gttggctgga aactccctgc caaaactatg ttcaacaata acccctggat aatactcctt
     1681 taggtagcga ggtatctgca ctgttccgat atcctcagat atccttgaat gattgcaggc
     1741 ttcagcgata agcactttat ctcctatctt cagagtatca agcgctttta ctccctcaac
     1801 aaaagtatcc agcctgcctc tgctcatatg gtttatcatc actatcgaaa aggttgtcag
     1861 catgatatca tcaaaaaccc acttggccat tatatccata gcctgcgaat cagttattat
     1921 tgcctttggc tttatccttt ggataaactc ttcaaatcta tttttctcat ctgcatcgcc
     1981 cttccttgcc tttgaaagat tcatgcgata agatactgga taagcccatc ttctggtgat
     2041 atattcctcg accatctgct gaggcctaag cagccgccct tgcggagtct cttcatccat
     2101 ggggatatta aggacgtaaa attgcccttc ttcaataaag ggcagcagct caaattgctt
     2161 gtttcctggc ttaaaattat ttaaaataaa ttcaagcaaa ggctgcctgc aggaagtatc
     2221 gatagccttg atccctattg atttgtagct gttaaataac ccatgcctgc tgtcttcatc
     2281 aaacacatta aatataacca ataactgttt gccaagcttc tttgcttcag acattatcat
     2341 ctcttcccag gcaaaatctt ccgatgaagg attcacggca agaagcgcaa ggtcacactc
     2401 ccttaggcaa ttgaacgttt ttgttctctt cttctcacca agcacaccat cttcatcgat
     2461 gccggctgta tcgaacagat tgacaggccc aagcccatgt atctcagcag gcatcgactt
     2521 gatatcagct gttgttccgg gttttacatc gacaatagag cattcctgtt gcgtgagaag
     2581 gttcatgata gtgctcttgc cgacgttcac tcttccaaag agccctatgt tatctctttg
     2641 tactatcatg ctctgtcacc ataacctgtt ccgatagtcc ttttgacaga cagtatcagc
     2701 cgctttacgc acccagagca atcgtcaggc tcctcataga tgcaagcctt gtttggatac
     2761 agttgatagt atcgtctata ctttcctggt gtgatattag gcatcaggag atttgctccg
     2821 catccaagcg caagctgcct gccgttggca aggtttaatg tctgtaaagc agttgtcgct
     2881 ggaataaaga catctttgca gacaagcctt gttactgcta tcatcttaag caccatctca
     2941 aagtcaccgt ttgcgcagtc aaaaagaggt gtatcgtggt gagataaaaa aggccccatt
     3001 ccaaccatgt cagccccgag ttgttttgca agtagtatat catccgccat atcttcgatt
     3061 gtttgtcctg gcagccctat catcgagcct gtgcccacct gataacctat ctgtttaaac
     3121 ttttttatta gatcaatccg ctctttaaaa gaattctttg tcattgaact attgggatga
     3181 agagacccat acaatttttc attcgatgtt tcaaacctta caaggcatct tcttgctcct
     3241 gcctcaaaaa accgcttgta gtcttcaatg cttctctcgc ctatgctaag agccaccctg
     3301 cagtcacact catttaatat ccctctgatt atctcttcaa tcatatcagc tgtatagcaa
     3361 tcatcttcac cagactgcag cagcactgtt ttaaatccaa gtttgtctac agcattttta
     3421 gctaggtcta ttatttgggc tttgtcaagc ctgtatctat ccaggtttag gttggatctc
     3481 cttatgccgc aataaagaca atcattcttg caatggttgg aaaactcaag tatgcccctg
     3541 atatgaacat cgtcactcaa gtgttctttt cttaccctat ctgcatattg atataatgat
     3601 tctagctcat cctttctttc aaggctaagt aaaaaaatga tgtcttcttt actcaagtct
     3661 tccttatgca ataccctcat catcttagaa atataagtct cgttttccat tttttatctg
     3721 ttccaaccgc tccataaata gccttttgat tttgctgtca gtgattttct ccatctcttt
     3781 tgctatcagc tcttgcccga ttttcttggc ctcgccgtcg ccatagtctt ccagat
//
LOCUS       DASRUK010000086         4607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_904646, whole genome shotgun sequence.
ACCESSION   DASRUK010000086 DASRUK010000000
VERSION     DASRUK010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904646"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..358)
                     /locus_tag="VFF28_03800"
     CDS             complement(203..358)
                     /locus_tag="VFF28_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44783.1"
                     /translation="MAHPINPTPILCGEDKEKFLEDIKSVSYDKDKETYLKECESIFN
                     HIKKEDI"
     gene            794..866
                     /locus_tag="VFF28_03805"
     tRNA            794..866
                     /locus_tag="VFF28_03805"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:828..830,aa:Asp,seq:gtc)
     gene            878..965
                     /locus_tag="VFF28_03810"
     tRNA            878..965
                     /locus_tag="VFF28_03810"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:913..915,aa:Lys,seq:ttt)
     gene            978..1217
                     /locus_tag="VFF28_03815"
     CDS             978..1217
                     /locus_tag="VFF28_03815"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit H"
                     /protein_id="HZX44784.1"
                     /translation="MKKFDVTTHILVPEHQKLNDKEKKELLEQFKISLKELPKISKKD
                     PAIQHLSPKEGDVIKIIRKSPTAGEAIFYRGVINV"
     gene            1210..2691
                     /locus_tag="VFF28_03820"
     CDS             1210..2691
                     /locus_tag="VFF28_03820"
                     /inference="COORDINATES: protein motif:HMM:NF007175.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B''"
                     /protein_id="HZX44785.1"
                     /translation="MYKYSKTLIKKHFKEQSFVNSNIASFNNFIENELKDIIEENKEI
                     IPTIPPQNIEDFRIKFDKIWVTKPEITEADGSKRNIYPAEARLRKLTYSAPTFIEVSA
                     HINGVQRESFTTQIGNIPIMLKSKNCHLNNLSRDELIQQGEDPDDPGGYFIINGTEKV
                     LISIEDLAPNRVLVEKGTGPSEFTGKLFSERGSFKIPHTLERMKDGIFYLTFTRVKRI
                     PIVVIIKALGMLKDEDIVNYVSKTQKYDEILINLFEFVDIKSEEEALDYIAKRIGITQ
                     AKEIRIERMKEILDKYLMPHLGINKEDRIYKSYNLCKFMKKYLDVSNGKLEIDDKDHY
                     MNKKLKLSGDLLADLFRVNLKVLVGDLLYNFQRIVKRGKFPSIKVIIRDKLLTQRIYS
                     SMATGNWVGGRKGISQRIQRLNYLESLSHLQRVVSPLSTSQENFEARELHCTHLGRLC
                     PIETPEGTNIGLRKNLALLCSISSEANEAELVKQLKSLGLKSI"
     gene            2688..4490
                     /gene="rpoB"
                     /locus_tag="VFF28_03825"
                     /pseudo
     CDS             2688..4490
                     /gene="rpoB"
                     /locus_tag="VFF28_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203701.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B"
BASE COUNT         1624 a          913 c          967 g         1103 t
ORIGIN      
        1 ctaaaaaatt tgccattgca aatttttggt gcaccaaaaa tcctatggat tttttagtgt
       61 ttcaagcgtc aaaaaccact taaccgtcat gccaccagtc tatagcgact gaaaggagcg
      121 gtgactggtg gtttttgaca actctagaaa gtttagtgat ttttacatcg gctaattcgt
      181 attttccatc cttcatggag aactaaatat cttctttttt aatatgatta aaaattgatt
      241 cacattcctt taggtaggtt tctttatctt tatcatagct tacgctcttt atatcttcaa
      301 gaaacttctc cttgtcctca ccgcataaaa ttggcgttgg gtttattgga tgtgccatct
      361 tgcttgtaaa tttatctttt tatttaaatt tatccattga aaagtagtaa acagaatatc
      421 ctcttgtttc atcctcattt tgcctcctct tttctacctt tcccctatag gataaatcct
      481 attagcctat tatataaagt ttattgtttg aaaagacttt gaaatttaaa tgctttagct
      541 tgcttcttta cttatccact taacactatc ttacccaaaa aacaaacttt tataaacaag
      601 cgcctagttc aaaagtggat aaatacaggg ggaattgggt ttaacttaca atatcacacc
      661 ttaaatgtta aaaaacgata ggtttataaa taacctcaaa tctcaatcgc tgatggcaga
      721 taatgtttct ttagagaata tactataagg ttaaatttta ttcactaaga aaaaagtctg
      781 gccaaaaaac taggccctgg tagtgtagtg gcctatcatg aagccctgtc gcggcttcga
      841 cccgcgttcg aatcgcggcc agggcgttta cactcatggg cccgtagctc agcctggtta
      901 gagcgcctca cttttaatga ggcggccgcg cgttcgaaaa aaacccgaaa atcgcgtcgg
      961 gcccgttatg ctaaaaaatg aaaaaattcg acgtaacaac acacatattg gttcctgaac
     1021 accaaaagct caatgataaa gagaaaaagg agctattgga gcagtttaag atttctttaa
     1081 aagagcttcc aaagataagc aaaaaagacc ctgctatcca gcatctatct ccaaaagagg
     1141 gcgatgtcat aaagataata agaaaaagcc cgacagcagg cgaggcgata ttctacagag
     1201 gcgttattaa tgtataaata ctcaaagaca ctgataaaaa aacactttaa ggaacaaagt
     1261 ttcgtcaact ctaacatcgc ttcttttaac aattttatag aaaacgagct gaaggatatc
     1321 attgaagaaa acaaggagat aatccctacc atccctcctc aaaacatcga ggacttcagg
     1381 attaagtttg ataagatatg ggtgacaaag ccagagataa cagaagcaga cggctcgaaa
     1441 agaaacatct atccggctga agcaaggcta agaaagctta cctacagcgc acctacattc
     1501 atcgaagtct cagctcatat caatggggtc caaagagagt catttacgac ccagatagga
     1561 aacatcccga taatgctgaa aagcaagaac tgccacctaa acaacctgag cagggacgag
     1621 ctcatccagc agggagaaga ccccgatgac ccaggaggct atttcataat caacgggact
     1681 gaaaaagtcc ttatcagcat agaagacctt gctccaaaca gggtcttagt agaaaaaggc
     1741 acaggcccat ctgaattcac aggaaaatta ttctcagaaa gaggctcgtt caagatccct
     1801 cacaccctgg aaaggatgaa agacgggata ttctatctaa ccttcacaag ggtaaaaagg
     1861 atccctatag tggtcataat caaagcatta ggcatgctaa aagacgaaga catagtcaat
     1921 tacgtttcaa agacacaaaa gtatgatgag atcctgatca atctatttga atttgttgac
     1981 ataaaatcag aagaggaagc tcttgattac attgcaaaga ggataggcat aacccaggca
     2041 aaagagataa ggatagaaag gatgaaagag atcctggaca aatacttgat gcctcacctg
     2101 ggtatcaaca aagaagacag gatatacaaa tcatataatc tttgcaagtt catgaagaaa
     2161 tacctcgatg tctcaaacgg caagttagag atcgatgaca aggaccacta catgaacaag
     2221 aagctaaagc tgtcaggcga tctactggca gacctattca gggttaatct aaaagtgctg
     2281 gtaggcgacc tcttgtacaa cttccagagg attgtaaaga gaggaaaatt cccatctatc
     2341 aaagtcataa tcagggacaa gctgcttacc cagaggatat acagcagcat ggcaacagga
     2401 aactgggtcg gcggaagaaa aggaataagc cagaggatcc agagactaaa ctatctggag
     2461 tcattaagcc atctccaaag ggtcgtctca ccattatcga ccagtcaaga aaatttcgag
     2521 gcaagggagc ttcactgcac acatcttgga aggctttgcc cgattgagac tccagaagga
     2581 acaaacatcg gcttaagaaa gaatcttgca ttgttatgct ctatatcatc agaagcaaat
     2641 gaagcagagc tggtaaaaca gctcaaaagc ttggggttaa agtcaatatg acagaagtat
     2701 acgtaaactc aaaattcgca ggagaaatag aagatgccag aactttcgta gagaagatta
     2761 aagaggacag gagaaaaaac aagatagacc acaatattaa cgtctatttt gatgataagg
     2821 cagacagcat tttcatcgag tcagggagag gaagggcaag gaggccattg atagttgtaa
     2881 aagacggcca gcctctgttg acagaaaaac atatcaagca gcttgaaaaa gacgagattt
     2941 tatggtcaga cctgataaag cagggcgtca tcgaatacat cgacgctgca gaagaagaga
     3001 actgctatgt ctcattcttt gattcagagc ttacccagga ccacactcat cttgaggtca
     3061 cacctcttgc gatgatgggc cttgcaggct cacttgtgcc ctatgcaaac tttaaccaat
     3121 ccacaaggct taacgctgga acaaaaaacc agaagcaatc attaggattc tatgtatcta
     3181 actattcagt aaggatggat atggatgtaa acctgttgta cgaacctcag gtccctatag
     3241 tccagacaat aatccatgag atttcagagt atgcaaaaca cccagcaggt caaaacatcg
     3301 ttgtcgctat catgtcctac aagggatata acatggaaga cgcaattatc ttaaacaaag
     3361 gctctatcca aagaggcctt gcaagaaact catacttcag gccatccatc gcagaggaat
     3421 taagatatgc tggaggcctt gtcgatgaag tttcaattcc agacaaggac gtcaaaggct
     3481 acaaatcaga aaaagattac agatatcttg aagcagacgg aataatctat ccagaagcag
     3541 agattgctga aggagatgtc atcataggaa agacctcccc gccaagattc ctttcaagcc
     3601 tggatgaata taaccttgct tcctcaacaa gaagggaaag ctcagtttcc acaaagcatg
     3661 gagaaaaagg agtggtcgat tttgttctta tcacagaaaa cgaagaagga aacaagcttg
     3721 tccaggtaag ggtaagggac gaaaggactc cagagatagg agataaattc acaagcaggc
     3781 acggacaaaa aggagttatc ggcctcatca tcccagaagg ggatgttcct ttctcagcat
     3841 caggaacaag gcctgatctg atattctcac cgcacggaat tccttcaagg atgacaattg
     3901 cacatcttat tgagatggtc ggtggaaaag ttggcgcact aaatgcaaga tatataaacg
     3961 gaacgacatt tgatgctgag ccagaagaaa agctaagaaa agagctgctt agcctgggtt
     4021 tcagggaaaa cggcactgaa acactatatg acggagagac aggagagcag tatcagacta
     4081 agatttatgt tggcaacatg tactatctaa agctaaagca catggtagca aacaagatac
     4141 actcaagggc aagaggccca atccaattat tgacaagaca gcctactgaa ggaagagcaa
     4201 aagaaggcgg attaagatta tgagagatgg aaaaagatac attcatcgct catggagcct
     4261 ctcttctttt gaaagaaagg tttgactctg acaagacaac tatcgcagtc tgtgaaggat
     4321 gcgggcttat tgccgtcagg gatgaattca agaataagag ctattgcccg atatgcggcg
     4381 acaactccga gatatcattc atcgaaattt catatgcatt caagctgata cttgacgagt
     4441 tcaaatcatt atgcatatat ccaaaacttc agcttaaggg caagtactag gaagaggaaa
     4501 aatggcagac gaagcagcac cagtagaaga agcaaaggaa aagatagagc aggcagaaga
     4561 aaagattatt caaaggatag agtctgaaga aataatcgta agcaaaa
//
LOCUS       DASRUK010000087         2836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_583450, whole genome shotgun sequence.
ACCESSION   DASRUK010000087 DASRUK010000000
VERSION     DASRUK010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583450"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1292
                     /locus_tag="VFF28_03830"
     CDS             132..1292
                     /locus_tag="VFF28_03830"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX44786.1"
                     /translation="MAKIDKNVYLLGIVSFLTDVSSEMIFAVFSIFFTVVLGASTVLL
                     GLIEGLADFAASSLDYISGVISDRTGKRRLPAAIGYGFSTLAKIMLVFANSAVLASIF
                     RVTERLGKSFRGPPRDAWLSSLTTDANRGLSFGVHKAFDKAGAVIGPFIAYFLLRALG
                     QSMGTFKLLFLIALVPAAASVIFLLFVKEKPEKKEKRENIFTAYRNLSSGYKHFLKTA
                     GIFSLAYFSFGFLLLKAYIVGFAIEEVVLLYALFNVAFVIFAAPLGRLGDRIGRKKLI
                     VIEYAIYILMSIGFIFASTKMAVIVLFVIFGIFYSIDESQSKVYIADLEKTKRGTAVG
                     LYNFFTGLIYLPASLIAGWLWKINPNYAFIFAGVVALGALAYFVKPRFELSG"
     gene            complement(1281..2423)
                     /locus_tag="VFF28_03835"
     CDS             complement(1281..2423)
                     /locus_tag="VFF28_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44787.1"
                     /translation="MFTVEVKQLEVNDRIDNLYILRGPNGQEARISGRAGSIEELLLP
                     PGGDDSLIRLVYGHASKLDFFVGRERSNVLPAYTEDYHRQISESRDAGLMQGGVPVTF
                     FPGRVQKGRIRFLQPDYSYAEFDVHPDHPPSDSLLHLGAGKDADFEFDSLKHDENSAS
                     IKLTCDIGSKLYGEGFPQKLRAEVEYTLGRDPLKRKVTLFNDSEIYVTIPTSDHSSCM
                     PGSAPLERCSLTMNAIGNYELAEKTKIATGRLIQPARFVRWGGIQLSSEIEEMYRLSP
                     GARDIRMTNEQTGEGLLITVDPKTNMAYVWTHNTTHDTYKHQFCSIQPYGGDPTAIVS
                     AVACNTPQELARMDPLFRQSIKRMMMPHSNISYETEWKAFKRRLAA"
     gene            complement(2487..>2836)
                     /locus_tag="VFF28_03840"
     CDS             complement(2487..>2836)
                     /locus_tag="VFF28_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HZX44788.1"
                     /translation="AARYVAKNIVAAGLADRCEVALSYCIGVAEPTSVNVETYGTNKI
                     PEEAISELVKKHFGLKPREIIEGLKLKRPIYRKTTNYGHFGREDPDFGWEKTDKAELL
                     RKEAGVTVKCVEH"
BASE COUNT          711 a          600 c          599 g          926 t
ORIGIN      
        1 gttcaattga tttctcggat aatcttgcat aacagggcaa gactatcata ttttaacagg
       61 attcagggat aatttgtgcg caaagccact actatggcaa catttattaa tccctaaact
      121 aaataatcat catggccaag atcgacaaaa atgtttatct gctgggcata gtcagctttc
      181 ttacagatgt ctcttctgag atgatatttg cggtgttttc gatatttttt actgtcgtac
      241 ttggagcatc tactgtgctg cttgggctta ttgaaggcct tgcagacttt gctgcttcct
      301 ctcttgacta tatatccggc gtcatatctg atagaacagg caaaaggaga ttacctgctg
      361 cgataggcta tggattttcg acgcttgcaa agataatgct tgtgtttgcc aattctgccg
      421 tccttgcatc aatctttaga gtgacagaaa ggttaggtaa gtcatttaga ggaccaccaa
      481 gagatgcatg gctgtcttca cttacaactg atgcaaacag aggactttct tttggagtcc
      541 ataaggcatt tgacaaagca ggcgctgtca tcggaccgtt catcgcttat tttctgctga
      601 gggctcttgg gcagagcatg ggtacgttta aactgctgtt ccttattgca ttagttcctg
      661 cggcagcgtc tgtgatattt ttgctgtttg tcaaagagaa gcctgagaag aaggagaaaa
      721 gagaaaacat ctttactgcc tataggaatt tgagcagtgg gtataagcac tttcttaaga
      781 ctgcggggat attctcgctt gcttatttta gcttcggatt tctattgcta aaagcttata
      841 ttgtcggttt tgccattgaa gaggttgtac ttctatatgc attgttcaat gttgcctttg
      901 taatatttgc cgcacctttg ggcaggcttg gagacagaat aggaagaaaa aaactgatag
      961 ttatcgagta tgcaatctat attctgatga gtataggatt tatcttcgcc tctaccaaga
     1021 tggctgttat cgtgctattt gtgatttttg gaatattcta ttctattgat gaaagccaga
     1081 gcaaggtata catcgctgat cttgagaaaa caaaaagagg gactgctgtc gggctttata
     1141 atttctttac agggctgata tacctgcctg cttcgctcat cgcagggtgg ctatggaaga
     1201 taaatccgaa ttatgctttt atttttgccg gagtggtcgc tcttggtgcg ctggcgtatt
     1261 ttgtgaaacc aagatttgaa ctaagcggct aatcttctct taaatgcttt ccattctgtt
     1321 tcataagata tattgctgtg cggcatcatc atccttttga tagattgcct gaatagcgga
     1381 tccatccttg ctaattcttg tggagtattg caggcaactg cgctgacaat cgccgtagga
     1441 tctccaccat atggctggat agaacaaaac tggtgtttgt aggtatcatg agttgtgtta
     1501 tgagtccata cataggccat atttgttttg ggatctacag ttatcaataa gccttctcct
     1561 gtctgctcat ttgtcatgcg aatatccctt gcaccagggg atagtcggta catctcctct
     1621 atctctgaac ttaattgaat accaccccat ctaacaaacc ttgcaggctg gataagcctg
     1681 ccggtggcta tctttgtctt ctctgccaat tcataattgc ctatagcatt cattgtcagg
     1741 ctgcatcttt caaggggtgc tgagccaggc atgcaggaac tgtgatctga tgttgggata
     1801 gtcacataaa tctcagaatc gttaaatagg gtcacttttc tttttaatgg atctcttccc
     1861 agggtatatt ctacttcggc tctcaacttt tgaggaaagc cttcaccata gagctttgag
     1921 ccgatatcgc atgtaagttt gatacttgct gaattttcat catgtttaag gctgtcaaat
     1981 tcaaagtcag catctttacc ggcccctaaa tgcagtaagc tatctgatgg gggatgatca
     2041 ggatgcacat caaactctgc atagctatag tctggctgta agaatctgat ccttcctttt
     2101 tgcactcttc ccgggaaaaa ggttactgga acgccacctt gcatcaaccc tgcatctctt
     2161 gattctgaga tttgcctgtg ataatcttcg gtataggctg ggagtacatt tgagcgctct
     2221 ctacctacaa aaaagtcaag cttgcttgca tggccataca ccaatcttat taaagaatca
     2281 tctcctcccg gaggaagaag caattcttct atggaacctg cccttcctga aatccttgct
     2341 tcctgaccgt ttggccctct tagaatatat aaattatcaa tacggtcatt gacttccagt
     2401 tgttttactt caacggtaaa catttttttc cccagagtag gaaaccaata aagctatata
     2461 aagattgctg gaaaaaagaa aaaaatttag tgctctacgc acttgactgt gactccagcc
     2521 tcttttctta ggagctctgc cttgtctgtc ttttcccagc caaagtcagg gtcttctctt
     2581 ccgaaatggc cgtagttcgt ggtcttcctg tagataggcc tctttagctt caatccttcg
     2641 attatctccc ttggctttag gccaaagtgc ttttttacaa gttcgcttat cgcttcttca
     2701 ggtatcttgt ttgtgccgta ggtctcaacg tttactgatg tcggttcagc tacacctatg
     2761 caataggata atgccacttc gcacctgtca gcaagccctg ctgcaacaat gttttttgca
     2821 acataccttg cggcat
//
LOCUS       DASRUK010000088         5604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1251378, whole genome shotgun sequence.
ACCESSION   DASRUK010000088 DASRUK010000000
VERSION     DASRUK010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251378"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..1036)
                     /locus_tag="VFF28_03845"
     CDS             complement(209..1036)
                     /locus_tag="VFF28_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44789.1"
                     /translation="MATSTLDKPKKTSIYQRSGLGPLPIHHVERLLHQAGRKVAERDL
                     TSLVDKFWSSHFDDEKGRLKEGMDDESAANAFLKTLGEYHYGPKLYKELIDNGHHEKV
                     KSELVETYGSHVEWDQLRKAFAAGYESRDHLQQSLKTPMVDRAVQTKQQRAQKAIVEY
                     KAQKSTRDELKKHLQMRAGDKDLDDTLLETLDVEYVAPFIERYMNIPGNLEKTEKNAK
                     VKLDIPIEVVGKPDFAKYFKDGKRYVQLAREMEINADSSHRLAPRGKEKTHGKASYN"
     gene            1182..3050
                     /locus_tag="VFF28_03850"
     CDS             1182..3050
                     /locus_tag="VFF28_03850"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZX44790.1"
                     /translation="MPKKSKSPEKKEQSEKIQDYKNIPQIAPGPMPLPQLSKEQEEKL
                     KEIKTKLDKFQKQVIEKFDKYIMGVALMPPPQPKQGEQIDKDRINVLILVDDSDSKKM
                     TKLELKDKLLAIVDKLAQDIDKNIYPDVLLLSELWQNCYDGKYDLLQLVALSAPVFDK
                     GMLQAVKIAEVHKNMVLKKFERYIVSYVLAGSLIQGRATPTSDIDVWVVIDDTDVKRM
                     TRAELKDKLRAIIIGMGIEAGDITGIKNKLNIQVYILTDFWESLKEANPIIFTLLRDG
                     VPFYDRGIFMPWKQLLRMGKIKPSAEAIDMFLASGEQMLNRVKTELKNIGMEDIYYAI
                     LTPSQAALMLHGVPPPAPKETAELMREIFVDKERLLEDKYVDILEKVIKNRKDIEHGL
                     KKDTTGKEIDEMLDDADKYLKRINRLFTQIERIKDARDMINTYESVVTIIRDVLKMEG
                     IERVADEEVVKLFEDELISEGKIPAKYLRILNDIIKAKKDYDDKKLTPTAAEQVKKDS
                     GSFIKFLVEYMQSKRGKEIERAKIRVKHGNKYGEVLLLGEHAFIIHDIDKEEKEISKG
                     KVGKDGTIESIERTTLEEMEKVLAKVEIPPKVFIREKVFENLKSIFGKDVEVLINY"
     gene            3090..3656
                     /locus_tag="VFF28_03855"
     CDS             3090..3656
                     /locus_tag="VFF28_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44791.1"
                     /translation="MPDEIPWKDEINEPATYIRYKGIWDMQDMYETIANFFRLKKYDF
                     YERVYKHKHPSPFGVERQYVWEAEKQENEFIHFIYDVYIHTYDAHDVEVVMPDNHKKI
                     YTKGRIWIELKAKVITDKYKRWQKKSFYSSLKSFYGKYLLKENFAQGWVPKIRYEMYE
                     LHSMLKARLKMEADEYEHANGAGVNRRF"
     gene            3657..4187
                     /locus_tag="VFF28_03860"
     CDS             3657..4187
                     /locus_tag="VFF28_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44792.1"
                     /translation="MSEIKIVVDHLRLSYTGTMQVKELFKIINRWSFERQLQKKEDRD
                     HEYNEPDGKHIEYEISHWKKISDYIKYIFKVRAIFKGIKKVEITMGKRKIKADHGTVT
                     FFFDGFVEYDYEHRWDERPMFVFLRSIYDKFIYRPYSDRFDDRLVKEVNDLKNELETF
                     LNMYRYHKVVSKAPYF"
     gene            4190..4690
                     /locus_tag="VFF28_03865"
     CDS             4190..4690
                     /locus_tag="VFF28_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44793.1"
                     /translation="MKIDKFVIDGMKCGYSGPFSVSDFFKKVETWMHEKGKETELKSK
                     REEVTEDSKNIEWTVEFWQDASEWSRIVIRIRALFKDVRDVKVKVGKAKKVMQKGNAI
                     ILIDGILETDVDDKWANKPTMQFMRTILDKFVYRFHMNKLEDRISKEAYETHDMITDF
                     FSKCHQ"
     gene            4738..4907
                     /locus_tag="VFF28_03870"
     tRNA            join(4738..4745,4764..4795,4832..4907)
                     /locus_tag="VFF28_03870"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4792..4794,aa:Leu,seq:taa)
     gene            5021..5392
                     /locus_tag="VFF28_03875"
     CDS             5021..5392
                     /locus_tag="VFF28_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44794.1"
                     /translation="MNKQKVLLDFEDFLNSYKDKKQADSSVNRFFPILSRNALNEDFA
                     FLIGKIMGDGHLDSNYTQKYIGKQHDLLELKKLIMNKFKIHPNRFSLVKRKSLGVSYL
                     LQLNYCPFGRILFLLGAPPRE"
BASE COUNT         1921 a         1004 c         1194 g         1485 t
ORIGIN      
        1 atgaatatga ttgaaagagt tttctcaaaa ttagacaaag acatcttaca aaacagcaat
       61 ttccaaaccg ttagagaagc aatgagcagg ataagcaact atttcgaaaa cgaaacaaga
      121 tttaagactt ggggtagtta agaaatattc tatgcaaata agaattcata gataaaatga
      181 ccaaacatga gaaaaataag gaataaaatt agttataact cgccttacca tgagttttct
      241 ctttccctct tggagcaagt cggtgagacg aatctgcatt gatttccatc tccctagcaa
      301 gctgtacata tctcttgcca tccttgaaat acttggcaaa atcaggctta cctacaactt
      361 caattggaat gtcaagcttt acttttgcat ttttttctgt tttttctaaa tttccaggta
      421 tattcatata tctttctatg aaaggagcaa catattctac atcaagtgtt tctagtaatg
      481 tgtcgtccag atccttatcg ccagctctca tttgaaggtg ctttttcagt tcatctctag
      541 tacttttttg agctttgtat tccactattg ctttttgggc ccgctgctgt ttagtctgaa
      601 cagcccgatc caccattggt gtcttaagtg attgctgtaa atgatcccta ctttcgtatc
      661 cagctgcaaa agcttttctt aattggtccc actcaacatg cgaaccatag gtctcaacca
      721 gttctgattt aactttttca tgatgtccat tatcgataag ttctttgtaa agttttggcc
      781 catagtgata ctcacccaat gtttttaaaa aagcatttgc ggctgactca tcgtccatcc
      841 cttcttttag tcggcctttt tcatcatcga aatgagaact ccaaaattta tcaaccagag
      901 atgtaaggtc acgttcagct acttttctcc ccgcttgatg aaggagtctt tcaacatggt
      961 ggataggtag tggaccaagt cctgatcttt gataaatgct tgttttcttg ggtttatcca
     1021 gtgtagatgt agccatgatt tttcaccaag caaaaaaagt aatcctttca atataaatct
     1081 ttctattttc agtatcataa aaccagtagt aaaaacaatc aaaagcttta aatatgtttc
     1141 agatatcttt ggcacttatc aaccgatagg gggtaaaatt aatgccaaaa aagtcaaagt
     1201 caccagaaaa aaaagaacaa tcggaaaaga ttcaggatta taaaaatatc cctcaaatag
     1261 ctccaggccc aatgcccctt cctcagcttt caaaagagca ggaagaaaag ctaaaggaga
     1321 taaaaacaaa gctggacaag ttccaaaagc aagttatcga aaaatttgac aaatatataa
     1381 tgggcgttgc tttgatgcct ccccctcagc caaaacaagg tgaacagata gacaaagata
     1441 ggataaatgt ccttattctc gttgatgatt ctgacagtaa gaagatgacc aagcttgagt
     1501 taaaagataa gcttttggcg attgttgaca agcttgctca ggacatcgac aaaaatatct
     1561 atccggatgt cctattgtta tcagaactat ggcagaactg ttatgatgga aaatatgacc
     1621 ttctgcagtt ggttgcgctc tcagcgccag tctttgacaa aggcatgctt caggcagtaa
     1681 agatagctga agtccataaa aacatggtcc ttaagaagtt cgagagatat atcgtctcct
     1741 acgttttggc aggctcactt atccagggaa gagcgacccc gacgtccgat attgatgtgt
     1801 gggttgtgat tgacgacaca gacgtaaaaa ggatgaccag agcagagctc aaagacaagc
     1861 ttagggcaat aataataggc atgggcattg aagcaggaga cataaccggc ataaaaaaca
     1921 agctaaatat ccaggtctat atccttacag acttctggga gagcctaaag gaagcaaacc
     1981 ctatcatctt cacattgcta agagacggcg ttcccttcta cgatagaggc atattcatgc
     2041 catggaagca gcttttaagg atgggaaaga taaagccatc cgcagaggca atagacatgt
     2101 tccttgcatc aggtgagcag atgctaaaca gggtaaagac agagctaaaa aacatcggca
     2161 tggaagacat ctactatgcc attttaacac catcacaagc agcgctcatg cttcatggcg
     2221 tcccgccgcc tgctccaaaa gagacagcag aacttatgcg cgagattttt gtagataaag
     2281 agcgcctgct ggaagacaaa tatgtagaca tacttgaaaa agtcatcaag aacagaaagg
     2341 atattgagca cggcttaaag aaagacacta caggaaaaga gatagatgag atgctggatg
     2401 atgcagacaa atacctaaaa aggataaatc gccttttcac tcagatagag aggataaagg
     2461 atgcaaggga catgataaat acatatgaat ctgtcgttac aataataagg gatgtcctta
     2521 agatggaagg catagagaga gttgcagatg aagaggtcgt caagctcttt gaagatgaac
     2581 ttatctcaga agggaagatc cctgccaaat atctcaggat attaaacgac ataatcaagg
     2641 caaagaagga ttatgatgat aaaaagctca ctccgacagc agcagagcag gtaaaaaagg
     2701 actcaggttc gttcataaaa ttcctcgttg aatacatgca gagtaagagg ggaaaagaga
     2761 ttgaaagggc aaagataaga gtaaagcatg gaaacaaata tggcgaggtt ctgctgcttg
     2821 gagagcatgc cttcatcata catgacattg acaaggaaga aaaagagata agcaagggca
     2881 aggtgggtaa ggacggcact atagagagta ttgaaaggac tacccttgaa gagatggaaa
     2941 aagtccttgc aaaggttgag atcccgccaa aagtctttat cagggagaaa gtctttgaaa
     3001 acttgaagag catctttgga aaagacgtgg aagtgctcat aaattattag gcaaaaactt
     3061 tatatattgt tctttgatta aagaagatta tgcctgatga gataccctgg aaagatgaga
     3121 taaatgagcc tgcaacctac atcaggtaca agggaatttg ggacatgcag gatatgtatg
     3181 agaccatcgc taatttcttc agattaaaaa aatatgattt ctatgaaaga gtgtacaagc
     3241 acaagcatcc ctcaccattt ggtgttgaaa ggcagtatgt ctgggaagca gaaaaacagg
     3301 aaaatgaatt cattcatttc atctacgatg tctatataca tacctatgac gcacatgatg
     3361 ttgaagtagt catgcctgat aatcacaaaa agatctatac aaaaggtcgg atctggattg
     3421 agcttaaagc aaaggttata accgataaat ataaaagatg gcaaaaaaaa tcgttctatt
     3481 caagccttaa atctttctac ggcaagtatc ttctcaagga aaattttgcc caaggatggg
     3541 tgccaaagat taggtatgag atgtatgaac tccactcgat gctaaaagca aggctgaaga
     3601 tggaagctga tgaatatgag catgctaatg gagcaggagt aaacaggcgg ttttaaatgt
     3661 cagagataaa gatagtcgtc gaccacttaa ggttgagtta cacagggaca atgcaggtta
     3721 aagagctctt taagataata aacagatggt cctttgaaag gcagctgcag aagaaagagg
     3781 accgcgatca tgagtataac gagcctgacg gcaagcacat agaatatgag atatctcatt
     3841 ggaaaaagat ctccgactat atcaagtata tattcaaggt gagggcgatc ttcaagggca
     3901 taaaaaaggt cgagataact atgggcaaaa gaaagataaa ggcagaccat gggacagtca
     3961 cttttttctt tgacggtttt gtggaatatg attatgagca caggtgggat gaaaggccga
     4021 tgtttgtctt cctgaggtct atctatgata agtttatata tcggccatac agtgatagat
     4081 ttgatgaccg gttagtaaaa gaggtaaatg acttgaagaa cgagttagag acattcctga
     4141 acatgtacag gtatcataag gtcgtctcaa aagcgcctta tttttaaaaa tgaaaattga
     4201 caaatttgtc atcgatggaa tgaagtgcgg atatagcggt ccctttagcg tctctgactt
     4261 ctttaaaaaa gtggaaacat ggatgcatga gaaaggcaag gagacagagc ttaagagcaa
     4321 aagggaggaa gtgaccgaag actcaaagaa catcgagtgg acagtggagt tctggcagga
     4381 tgcaagcgaa tggtcaagga tagtcatcag gataagggcc ctgtttaagg acgtcaggga
     4441 tgttaaggtg aaggtaggta aggcaaaaaa ggtcatgcag aaaggaaatg ccataatcct
     4501 gatcgatggg atattggaga cagatgttga tgataaatgg gcaaacaagc caactatgca
     4561 gtttatgcgc acaatacttg acaagtttgt ttaccgcttc cacatgaaca aactggagga
     4621 taggatctca aaagaagcat atgaaaccca tgacatgata acggatttct tcagcaagtg
     4681 ccatcaatga aaagttttaa ataagtacct ttcattctct aaggacaccg ggcaattgcg
     4741 agggtaagct tgttgctcct cagagcgaag cctggtcaaa cgcgcaggac ttaaggtttg
     4801 ccatgatgga gcacccatct gtaaatgggg tatcctgttc cttagtggat ccgtgaaagg
     4861 attagcgatg ctagttcatc ggaaaggttc aaatcccatc cctcgcatac ttaatgtaag
     4921 gtcccttggg actaatagga acaggggcct tattttttct tcaataaact atttaaacct
     4981 caaaatctac ctttttgtta ttccaaatag tggctattct gtgaataagc aaaaggtatt
     5041 attggatttt gaagatttct taaatagtta caaggataaa aaacaagctg attcttcagt
     5101 taataggttc ttcccaattc tttccagaaa tgcactcaac gaggattttg ctttccttat
     5161 tggtaaaata atgggcgatg gccatttaga ttctaactat actcagaagt atataggtaa
     5221 acaacacgat ttgctggaac tcaagaagtt gatcatgaac aagttcaaga ttcatccaaa
     5281 tagattctcc ttagtcaaaa gaaaaagtct tggagtatct tacttattac aattaaacta
     5341 ttgccctttt ggaagaatat tatttctctt aggagccccc ccaagggaat aaaaccgaaa
     5401 agaagtttct aataccaact tggattttag aaaataaaaa atgcaaacga agatttttac
     5461 aagcactttt agaagatgaa cttagcacta tcaaaatcga aaagtgcaat tactccaaca
     5521 aacctaggct aaaagtgaaa ctctccagcc taacggctga agcctctttc taataatcta
     5581 ttgcattgat gcccttgggg cata
//
LOCUS       DASRUK010000089         5272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1251414, whole genome shotgun sequence.
ACCESSION   DASRUK010000089 DASRUK010000000
VERSION     DASRUK010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 5272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5272
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251414"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            433..768
                     /locus_tag="VFF28_03880"
     CDS             433..768
                     /locus_tag="VFF28_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44795.1"
                     /translation="MCRRAFKKEQAILLDICIPLAFEDGNFENRLFNYAMMHLIPNKR
                     NITFLANRLDQVTEDRPTTNAEVKKYAEHPMEGTMGIRIQKEMLGNYLAIAEKLVEQA
                     NARQSLSPT"
     gene            816..1061
                     /locus_tag="VFF28_03885"
     CDS             816..1061
                     /locus_tag="VFF28_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44796.1"
                     /translation="MYVARDHLTTPEVRHLFNTGYARQALFVPDYLAIVKHKAGFELR
                     YYPRPDSQVYDLFEPLGTLKELGVHVTSDCSLEEMLI"
     gene            1121..1873
                     /locus_tag="VFF28_03890"
     CDS             1121..1873
                     /locus_tag="VFF28_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44797.1"
                     /translation="MIPDLNDFTDKCMDLCARIGSRLSVGQKKIIGAANSSLQVCAGR
                     ESEVNLETLSAVCGQLSSATVLSSTGFDADKLPTNKEIYSYLTTDNKTAFRPKLQAYI
                     ALSGAYDDNELWCDSLYSRIVSSSRWIDPLSAQQRTVIASLIPKCFLNGETERYQSAL
                     DVPLLLEVCEEGRNAVAIMNRLSALVPDDSDGWLTDEMAISAIKGRQKTRPILGKGHV
                     YKKLVLDYLERKNGYSPDKAETYVPNKTYMPI"
     gene            1924..3141
                     /locus_tag="VFF28_03895"
     CDS             1924..3141
                     /locus_tag="VFF28_03895"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HZX44798.1"
                     /translation="MAKEKIILAYSGGLDTSVILKWLVDKGYDVVAYIADVGQNEDYK
                     AAEQKALKIGASKVYIEDLKKEFVSDFIFKAIKSNAVYEGKYLLGTSLARPLIAKKQI
                     EIAKKEKTNIVAHGATGKGNDQVRFELTYYTLMPDVKIIAPWKEDEFLSAFQGRPDMI
                     EYCKKHNIPVKATVKKPYSTDPNLMHISYESGILEDVSKGPADDMFEMTVSPKDAPDK
                     ETSLIIEFDKGIPIKVTDETNKVTETGPLNLFVYLNKVGGANGIGRVDLVENRFVGMK
                     SRGVYETPAGTILMQAHLDMEGITMDREVKLIRDSLIPKIASLIYNGFWYSPEFELLM
                     AMVDKSQENVSGKVMLKLYKGNVIITGRSSPNSMYDEKIASMDIHGGYDQKDAKGFIK
                     LNALRLKAWARKK"
     gene            3341..4414
                     /gene="ribD"
                     /locus_tag="VFF28_03900"
     CDS             3341..4414
                     /gene="ribD"
                     /locus_tag="VFF28_03900"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007953018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HZX44799.1"
                     /translation="MISHRKYMEITLKLAEKGKGNVSPNPMVGAIIVKRGRIVGKGWH
                     KKCGEAHAEVCALREAGKKAREGILYCNLEPCSHWGRTPPCTEQIVDAGIREIVVAMK
                     DPNPLVNGFQELKQRGIKSRIGILEEEARKLNEPYIKWMKTKKPFVVVKAAMSLDGQI
                     ATRTGDSKYITGREARKFVHELRAEYDAVMVGSNTVKKDNPELTVRLAKGRNPIKVIV
                     DSSLKLPINSNIVKNEPSKLIIATSKKASKAKVKELMLKGVHVLYINTIKGKLNLEHL
                     MKELGKREICSVLIEGGAELNAEAIKAGVADKVLFFISPQFIGKGLSALGDLGIAKVD
                     RSIKLKKLDYRKIGKDILIEGYL"
     gene            4450..5052
                     /gene="ribB"
                     /locus_tag="VFF28_03905"
     CDS             4450..5052
                     /gene="ribB"
                     /locus_tag="VFF28_03905"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HZX44800.1"
                     /translation="MFSKIPDAIKQLQRGDFIILVDDENRENEGDIVLAAEKATPSKL
                     NYIIQWAKGLMCLPIEAKRLDQLRIPMMVPDATDKFHTPFTVSVDAKRNTTTGMSVND
                     RLETIKVILNQKSKPEDLARPGHMFPLRAREDGVFDRQGHTEASVDLCKLAGLYPMAV
                     IAEIMNDDGTMAKLPDLELFAKKHKLSIYTIKDLIEYRNS"
     gene            5033..>5272
                     /locus_tag="VFF28_03910"
     CDS             5033..>5272
                     /locus_tag="VFF28_03910"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HZX44801.1"
                     /translation="MSIGTVEPGNIVDKSAWSKETMKIKNHSLYLILTEEFCNGKNLL
                     TIAKEAIDAGIDILQMREKHKSKEELIELGKKMSAL"
BASE COUNT         1764 a          971 c         1213 g         1324 t
ORIGIN      
        1 atgaatatga ttgaaagagt tttctcaaaa ttagacaaag acatcttaca aaacagcaat
       61 ttccaaaccg ttagagaagc aatgagcagg ataagcaact atttcgaaaa cgaaacaaga
      121 tttaagactt ggggtagtta acactgtatt tgcataccat ccaaggcctg gttcagtcga
      181 tcagcctatt gaaaagctga ttcacaggaa tgttgaaaag gagtggcggc aaccagcaat
      241 gtacgactat gtcaagttag tgtattcagg ctctgatcca gcaccaggga aacgacgcct
      301 gtcctggacg actaggttca cagataaagg gttgcgggcc ctggcagaat atttatcaaa
      361 atttggtact catcaagacc agttggaatt ggtcaaagat tcaaggatta ttcagcgtct
      421 ttattcccta gcatgtgcag aagagccttt aaaaaagaac aagccatact gttagatatt
      481 tgcatcccac ttgcttttga agatggaaat tttgaaaata ggctgtttaa ttatgcgatg
      541 atgcatctta tacctaataa aaggaatatc acctttttgg caaataggtt ggatcaagtt
      601 acagaagaca gaccaactac taatgcagaa gtaaaaaaat atgcagaaca ccctatggag
      661 ggtaccatgg ggattaggat tcaaaaggag atgttaggaa attatttggc aatcgcagaa
      721 aagctggtcg agcaggcgaa tgcaaggcag agcttgagcc cgacttaaag atttcttatt
      781 taaataactt ccagttactt agttgggtgg tagagatgta tgttgcaaga gaccatttga
      841 ctacacctga ggttaggcat cttttcaata ctggttatgc aaggcaggct ctttttgttc
      901 cagactatct tgctatagtc aagcataaag cagggtttga gcttaggtat tacccaaggc
      961 cggattcaca ggtctatgat ttgtttgaac ctttaggcac ccttaaagaa cttggcgtgc
     1021 atgtcacttc tgactgcagc cttgaggaga tgctgatata gttactaaca agtggtataa
     1081 atcagtaagt tttaaaaaga agcgctgctt tctcgagtat atgatacctg atttaaatga
     1141 ttttacagat aaatgcatgg atttatgtgc caggatcggc agtcgtttgt cagttggtca
     1201 gaaaaaaatt atcggcgctg caaatagctc attgcaggtt tgcgctggaa gggaaagcga
     1261 agttaacctt gagacgctgt ctgctgtgtg tggtcagctt agttctgcca cggtattgtc
     1321 gtccacaggt tttgatgcag ataaattgcc taccaataag gagatctaca gctacttgac
     1381 aacagacaac aaaacagcat ttagaccaaa acttcaagct tatattgcgc tgtcaggagc
     1441 gtatgacgat aatgaactat ggtgtgattc cttgtatagc cggattgtgt catcaagcag
     1501 atggatagat cctctaagcg cccagcaaag gacagtcatt gcttctctga ttcccaaatg
     1561 ctttctcaat ggtgaaactg aaaggtatca atcagcactg gatgtaccct tactcctgga
     1621 agtctgtgag gaaggaagaa atgcagtagc gataatgaac cgtctatctg cccttgtgcc
     1681 tgatgatagt gatggctggt tgactgatga aatggcaatc tcagcgatca aaggtaggca
     1741 gaaaaccaga ccaatattgg gaaaaggcca tgtgtataag aaacttgttt tggattacct
     1801 tgagcgtaaa aatggctact ctcccgataa agcagagaca tatgtcccaa ataagacata
     1861 tatgcctatc taattctagt accggtcaat aaatttataa atagccaaac gttaaatccg
     1921 ttgatggcaa aagaaaaaat tatacttgca tattccggcg gcctagacac atctgtaata
     1981 ctaaaatggc ttgttgataa gggctatgat gtagtggcat acatcgctga tgtcggccag
     2041 aacgaagact ataaggcagc agaacaaaaa gccctaaaga ttggagcttc taaggtttac
     2101 atcgaagacc taaagaaaga gtttgtcagt gactttatct tcaaggctat aaaatcaaat
     2161 gctgtctatg aaggcaaata cctgttaggt acatccctgg caaggccatt gatagcaaaa
     2221 aagcagatag agattgcaaa aaaagaaaag accaacattg ttgcacacgg cgcaacaggc
     2281 aaaggaaacg accaggtacg ctttgagctg acgtactata cattgatgcc ggacgttaag
     2341 ataatcgctc cctggaaaga agacgaattc ttgtcagcat tccaaggcag gcctgacatg
     2401 atagagtact gcaagaagca taatatccct gtaaaagcta cagtaaaaaa gccgtatagc
     2461 acagacccta acctgatgca tatcagctac gaaagcggta ttttggagga tgtatctaaa
     2521 gggcctgctg atgatatgtt tgagatgaca gtctcaccca aagatgctcc agacaaagag
     2581 acatcattga tcatagaatt tgataaagga atccctataa aagttactga cgaaacaaac
     2641 aaggtgaccg aaacaggccc attaaatctc tttgtttatc taaacaaggt tggcggtgca
     2701 aacggcatag gccgtgttga tcttgttgaa aatcgctttg tcggcatgaa atcaagggga
     2761 gtctatgaaa ccccagcagg gactatcttg atgcaggcac accttgatat ggaaggaata
     2821 accatggaca gggaagttaa attgatacgt gacagcctta tcccaaagat tgcttccttg
     2881 atctataatg gcttttggta ttcacctgag ttcgagcttt tgatggctat ggtagataaa
     2941 agccaggaga acgtcagcgg caaagtcatg ttaaagttgt ataaaggaaa cgtgataatc
     3001 accggaaggt cttcgcctaa ctcaatgtat gatgagaaga tagcgtctat ggacatccat
     3061 ggcggctatg accagaaaga tgcaaaagga tttatcaagc tgaacgctct aaggctgaag
     3121 gcttgggcga gaaagaaata gttaaagctg gtcgaaggcg atccttcggg ctcttatttt
     3181 ctttatcggt tccaaaaaaa atgccgggag aggatcttat taatcgaatg gcattgcttc
     3241 atgatttttg tgcaattctt tatctttcta tattttgcat tatattgagt agtataaaaa
     3301 ccgagttttt atatactatc tcttttattg ccatctatat atgatttccc accgcaaata
     3361 catggaaata accttgaagc ttgctgaaaa aggcaaggga aatgtctcac caaaccctat
     3421 ggtcggcgcg atcattgtga agagaggcag gatagtgggt aaaggctggc ataagaaatg
     3481 cggagaagcc catgctgaag tctgcgccct aagggaagct ggaaagaaag caagagaagg
     3541 catactctat tgcaaccttg aaccttgctc tcattgggga aggacccctc cttgtacaga
     3601 gcagatagtc gatgcaggta tcagagagat agttgttgcg atgaaagatc caaatcctct
     3661 tgtcaacggc tttcaagagc taaagcaaag aggcataaaa tcaaggatag gcatattaga
     3721 agaagaggca agaaagctaa atgagccata tatcaaatgg atgaagacaa aaaagccttt
     3781 tgtcgtagta aaagcagcaa tgtctcttga cggccagata gccacaagga caggcgactc
     3841 aaaatacatc acaggaagag aggcaagaaa atttgtccat gagctgagag ctgaatatga
     3901 cgccgtcatg gtcgggtcga acacagtcaa aaaggacaat ccagagctta ctgtaaggct
     3961 tgccaaagga agaaatccga taaaggttat cgttgacagc agtctaaaac taccgataaa
     4021 ttccaacatc gtaaaaaatg agccctcaaa gctcatcata gctacctcaa agaaagcctc
     4081 taaggcaaaa gtaaaagagc ttatgctcaa aggcgttcat gtcttgtata tcaatactat
     4141 aaaaggaaag ttaaaccttg agcaccttat gaaagagctc ggcaaaagag agatatgctc
     4201 tgtcttgata gaaggcggcg cagagctcaa cgccgaggcg ataaaagcag gcgttgcaga
     4261 caaggtcttg ttctttatat cgccccagtt tatcggcaaa gggttgagcg ctttaggaga
     4321 tctaggtatc gccaaggtcg acaggtcgat aaagctaaaa aagcttgatt atagaaagat
     4381 agggaaagat attctgattg aaggttatct ataggatact tttttaaata ataaacattt
     4441 cttttgatta tgtttagcaa aatccctgac gcgataaagc agctccaaag aggagacttc
     4501 atcatattgg tcgacgatga gaacagggaa aatgaaggag atattgttct tgctgcggaa
     4561 aaagcgactc cttccaagct aaactacatt atccaatggg caaaaggcct tatgtgcctt
     4621 cccatcgagg caaaaaggct tgatcagctg aggattccca tgatggttcc tgatgccaca
     4681 gacaagtttc atactccatt tacagtatct gttgatgcaa aaagaaatac gacaacaggc
     4741 atgagtgtaa acgacaggct ggaaacaata aaagtcatcc taaaccaaaa aagcaagcca
     4801 gaggacttgg caaggccggg gcatatgttt ccattaagag caagagaaga cggagtgttt
     4861 gataggcaag gccatacaga agcatcagtt gacctatgca agcttgcagg tctttatcct
     4921 atggcagtta tcgcagagat aatgaatgac gatggaacta tggcaaaatt gcctgaccta
     4981 gaacttttcg ccaaaaagca taagctttct atatatacga taaaggattt gattgagtat
     5041 aggaacagtt gaacccggca atattgtgga taaatcagcc tggagcaaag aaaccatgaa
     5101 gataaaaaat catagcctgt atctgatttt aacagaagaa ttctgcaatg gaaagaattt
     5161 actaacgata gcaaaggaag cgatagatgc aggaatagat atcctgcaga tgcgggagaa
     5221 acataagagt aaagaggagc tgatcgaatt gggaaaaaag atgtccgcat ta
//
LOCUS       DASRUK010000090         3951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2084364, whole genome shotgun sequence.
ACCESSION   DASRUK010000090 DASRUK010000000
VERSION     DASRUK010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2084364"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="VFF28_03915"
     CDS             complement(<1..1118)
                     /locus_tag="VFF28_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007788870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HZX44802.1"
                     /translation="MKVKIAASCPESSMNLGGIFLVKGNDFEDVPEIPEIVQAIDNGY
                     LETLVEKKEVKKEIRQGEELVIFAEQRNGKIARVSLELLGKARELALKLNVKTAALLL
                     VEKDNGVSKELIAYGADKVYVAENILLKNYQTSTYSKVVSEQIEKIKPSVVLFGATHI
                     GRDLAPRIAQRINTGLTADCTGLDITDDGLLLQTRPAFGGNLMAQIICKKQRPQMSTV
                     RPGVMKMLSFDGNRKGEILPVAVNITEKDMAVRVLEIVKSAKKKVNLEEAEIIVAGGR
                     GLSKKENFKMIEELASLLHAEIGSSRGPVDLGWVGKEHQVGQTGKTVRPRLYIAIGIS
                     GAIQHRAGMQNSEMIIAINKDASAPIHTVADYSIVDDL"
     gene            complement(1118..1912)
                     /locus_tag="VFF28_03920"
     CDS             complement(1118..1912)
                     /locus_tag="VFF28_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HZX44803.1"
                     /translation="MNIIVCLKQVPDTNEVKIDPRTGTLIREGVPTIMNPDDKHALIE
                     AVRIKRENGGKVTALSMGPPQAEEALREALAYGADEAVLLCDRAFAGSDTWATANALG
                     GAIKSLGNFDILFFGRQAIDGDTAQVGPQVAEYLKIPQITYAQKIEIGGGKLIVERQM
                     EEGYEIIEAKVPCLVTCISEINQAQYPTLFGIREAYRKKEVKVLSADTAKVDKTKVGL
                     LNSPTSVKKSFTPAGKGGGQTLKGSPKEVSAKIINMLKEKELVGGF"
     gene            complement(1913..3067)
                     /locus_tag="VFF28_03925"
     CDS             complement(1913..3067)
                     /locus_tag="VFF28_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZX44804.1"
                     /translation="MNFDFTPEQLAIKKMITSFVNSEIKPYADDADKLEIFPAEQLSK
                     LAKLGVMGMSIPEKYGGSELDNVSQTIIMEEVSRGCSAAAVSMGAHSSLTCHPLVNFG
                     TEEQKKRFLPKLASGEYLGAFALTEPNAGSDAVNVQTTAMREGDEYVINGNKIFITNG
                     GKADLINFVATTDKSKKHHGLSVFTIETKGLKGFELGKKEDKLGLRGSNTQELVFKDM
                     RVPKENLIGGVEGEGFKVSMSVFNSGRIGIGVQGVGIAQAAFDEALKYVKEREQFGMK
                     LHKFQALQFMLSDMATKIDAARLLCYRAAFVKDKGRRYVREAAMAKLYGSDVAMEVTR
                     VALQIHGGYGYIKDYPLERFYRDAKITEIYEGTSEIQRLVIAAELLGRGF"
     gene            3117..3482
                     /locus_tag="VFF28_03930"
     CDS             3117..3482
                     /locus_tag="VFF28_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44805.1"
                     /translation="MLGRILKIRYIKKAIAFSYMQITPSYVNSFVKATEKEDVSSICT
                     LVCKHPDLVRYPKARDWLDEAYTILLQRDRRVNVGLPLILYQAVKGSDLPDRKEILCN
                     FAIMSVVDNLYRSGYRRYS"
     gene            complement(3570..3710)
                     /locus_tag="VFF28_03935"
                     /pseudo
     CDS             complement(3570..3710)
                     /locus_tag="VFF28_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329349.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CxxC-x17-CxxC domain-containing protein"
BASE COUNT         1033 a          958 c          709 g         1251 t
ORIGIN      
        1 aacaagtcat ctacgatact gtaatcagca actgtatgta tgggtgcaga agcatctttg
       61 tttatagcga ttatcatctc agaattctgc atgcctgctc tgtgctgtat agccccagaa
      121 atccctatcg caatgtaaag cctcggccga acagtcttgc ctgtctgccc tacctgatgc
      181 tctttaccaa cccaccccag gtcgacaggt cctctgcttg aaccaatctc tgcatgcaac
      241 aaagatgcaa gctcctctat catcttgaag ttctcttttt tagaaagccc tcttccgcct
      301 gccactatta tttctgcttc ttcaaggtta accttctttt ttgcagattt gacaatctca
      361 agtaccctta cagccatatc tttctcggtg atatttacag ctacaggtaa tatctcccct
      421 tttctatttc catcaaatga gagcatcttc atgacacctg gccgaacagt tgacatctga
      481 ggcctctgct tcttgcagat gatctgcgcc ataaggtttc cgccaaaagc aggtcttgtc
      541 tggagcaata atccatcatc agtgatatca aggccagtgc agtcagcagt aagccctgtg
      601 tttatcctct gggcgatcct tggagcaagg tcccttccta tatgcgtcgc accaaacaag
      661 acaacagagg gtttaatctt ttcaatttgc tcactgacaa cctttgaata agttgaagtt
      721 tgataatttt ttaagaggat attttcagca acatacacct tatcagcgcc ataagcgata
      781 agttctttgc tcacaccgtt atctttctca acaaggagca gtgcagcagt tttaacattt
      841 agttttagag caagctctct tgccttgcca agaagctcaa gtgaaactct tgctatcttt
      901 ccatttcttt gctctgcaaa tatgactaat tcttctcctt gccttatctc tttctttacc
      961 tctttctttt cgacaagagt ttccaggtat ccgttatcta tcgcctgcac tatttctggt
     1021 atttcaggaa catcctcaaa gtcatttccc ttgacgagaa agattccccc aaggttcatt
     1081 gaactttcag ggcagctggc tgcaattttt accttcacta aaaaccacct accagttctt
     1141 tttcttttag catgtttata atcttggctg agacttcctt tgggcttccc ttaagtgtct
     1201 ggccgccgcc ttttcccgca ggagtaaatg atttcttgac agatgtagga gaattaagga
     1261 gtccgacctt tgttttgtcg actttagcgg tatcagcact caggactttc acttcttttt
     1321 tcctgtaagc ttcccgtatt ccaaaaagag taggatattg ggcttgattt atctctgata
     1381 tgcatgttac aagacagggg acctttgctt cgataatctc atagccttct tccatctgtc
     1441 tttcgactat gagttttcca ccgcctatct ctatcttctg ggcataggtt atctgaggta
     1501 tctttagata ttcagctacc tgaggtccga cctgtgctgt atcaccgtcg attgcctgtc
     1561 ttccaaagaa aagtatgtca aaattaccaa gtgattttat tgcgccgcca agcgcatttg
     1621 cagttgccca tgtatcagag ccagcaaagg ccctgtcgca taacagcaca gcttcatccg
     1681 cgccatatgc cagtgcttca cgaagcgcct cttcagcctg cggcggtccc attgaaaggg
     1741 cagtcacttt acctccattc tccctcttta tccttacagc ctcaataagt gcatgcttat
     1801 cgtcaggatt catgatggta gggactcctt ctcgtatgag cgtgcctgtc cttgggtcta
     1861 tcttgacctc atttgtgtca ggtacctgtt ttaagcatac tatgatattc atttaaaatc
     1921 ctcttcccag aagttcagca gcaatcacaa gcctctgtat ctcagatgtg ccttcatata
     1981 tctctgttat cttagcgtca cggtagaatc tctcaagagg ataatccttg atatagccat
     2041 agccgccatg tatctgtagt gccacccgag ttacctccat tgcaacatcg ctgccataca
     2101 gttttgccat tgcagcctca cgaacatacc ttctgccttt atctttgaca aaagctgccc
     2161 tatagcagag caaccttgca gcatctatct ttgttgccat atcagaaagc atgaattgta
     2221 atgcctggaa cttatggagt ttcatgccaa actgctccct ttctttcaca tatttaagcg
     2281 cttcatcaaa agcagcctgt gcgattccta ccccttgaac acctatgcct atcctgcctg
     2341 aattaaaaac actcatggac accttgaaac cttcaccttc aacaccgccg ataagatttt
     2401 cctttggcac cctcatgtcc ttaaaaacaa gctcttgagt gttcgaaccg cggaggccaa
     2461 gcttgtcttc ttttttccca agctcaaatc cctttaaccc ttttgtttcg atagtgaaaa
     2521 ctgagaggcc atgatgcttt ttgctcttgt cagttgttgc aacaaagttg atgaggtctg
     2581 cttttccgcc gtttgtgata aatatcttgt ttccatttat tacatactca tctccttctc
     2641 gcatagcggt tgtctggaca tttacagcat cgcttccggc atttggttca gtaagtgcaa
     2701 aagcaccaag atactcgcca gatgcaagtt ttggaaggaa cctctttttc tgctcctcag
     2761 tgccaaaatt gaccagagga tggcaggtaa gtgaagaatg tgcacccata gagacagcag
     2821 cagcactgca tccccttgag acttcttcca ttattattgt ttgagaaaca ttatccagct
     2881 cactgccgcc atatttttca gggatgctca tacccatgac accaagctta gcaagctttg
     2941 atagctgttc agcaggaaat atctcaagct tgtcagcatc atcagcataa ggctttatct
     3001 cagaattgac aaacgaagtg atcatcttct ttatcgcaag ctgctcaggc gtaaagtcaa
     3061 aattcatggt attagggaat cccaagggct atataaagat tgctttgact tattatatgc
     3121 ttggtaggat attaaagatt agatatataa aaaaagccat tgcattttct tatatgcaga
     3181 taactccctc atatgttaat tcttttgtca aagccacaga aaaagaggat gtgtcgagta
     3241 tctgcacgct agtatgcaaa catcctgatt tagtgagata ccccaaggca agggattggt
     3301 tagatgaagc atatactatt ctactccaga gagatagaag agtcaatgtg ggattacccc
     3361 tcattcttta tcaagctgta aaaggatcag atttgcctga cagaaaggag atactgtgta
     3421 attttgcaat tatgagtgtt gtggataatt tatacagatc aggctataga agatattcct
     3481 aagtcataaa aaaaataaat aaaaaaagaa gcagtttaga atcttggctt aaactctctt
     3541 cttccgccgc caccgccacc attgccgcca aatcttggct tcctatgctt ttgatagcaa
     3601 tctctacaat agactggcct cccttcctgc ggtttaaagg gaacctcgca ttcgttgcca
     3661 caatctgcgc acgtggcttt gtgcatctct ctctcaaatc caccttccat cttctataca
     3721 ccttttgctt tagttatact tcaaactcaa atgttttctg tatactgagt caacaggaag
     3781 catttgcact atttaaaact atgcatttag agtaagtatt cagtcatata taaactaact
     3841 tcttaggact atatgttcca tcaccgaaat ctttatttag ttatagtata tattaatata
     3901 tatcatgcgt aaattcaagt tccaagatgg caaatttacc cttgaatcag a
//
LOCUS       DASRUK010000091         2473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_587931, whole genome shotgun sequence.
ACCESSION   DASRUK010000091 DASRUK010000000
VERSION     DASRUK010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587931"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..805
                     /locus_tag="VFF28_03940"
     CDS             191..805
                     /locus_tag="VFF28_03940"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HZX44806.1"
                     /translation="MPEYLAFDKCIKLLKKHKIALSPYFILSSKEQAISVAASVGYPL
                     VIKAYADGIVHKFKEGYVKTGISSEDELSKEYTSISKKAKARKNCAIVIQKEAKGFEM
                     ILGAKLDPTFGYVVLLGLGGIYTEYLKKFSMRVLPITSKDAADMVEEMQIITGRIAKQ
                     KVANLILSISKMAVKEKMLELDLNPVIIGKDDINIADVRILTWP"
     gene            796..2178
                     /locus_tag="VFF28_03945"
     CDS             796..2178
                     /locus_tag="VFF28_03945"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HZX44807.1"
                     /translation="MALNDLFNPKSVAVIGASRDTKSAGYGILKNLVEGSFLKCRYGK
                     PFGGKVYAINPNADEILGKNCFPSIARISEPIDLAIIALPVKIVEKEVDNCIKKKVKS
                     IILISTGFAEIGKEGKAIQDRIAGKLKKRKIDLLGPNCLGIIRTSTHLNATFAPSMPP
                     PGKIAFISQSGALANSVIDWAIEERYGFSSIVSIGNQADKDIPDFIEYFARDEETKVI
                     TLYIEGLKDGKKFLNIAKKAKKPIIAIKSGKTAHGKSAVSSHTGSLAGDYEVYQAAFK
                     QAGVILAESVEEMFDIAKALASQPIPTKNAIAIVTNGGGPGVLCADYCSENGVNLITL
                     EKNTIKELESSNLMHPAFSRSNPLDIVGDALPERYKFAINTLLSKDYIAGLIIIQTLQ
                     TMTDSAENARAIVEASKKYPDKPIICTYIGGKFSRQSIIYLEANSVPDYNDVRKSAKV
                     MAALVGRIKR"
     gene            complement(2168..>2473)
                     /locus_tag="VFF28_03950"
     CDS             complement(2168..>2473)
                     /locus_tag="VFF28_03950"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZX44808.1"
                     /translation="SDASVIAVGVPYMMLDALTFPTMAVAMIASRVMQAMGYGLPGFV
                     INAVRVFVVAIPLAYFFVFVMGYGYLSIAIAMIMGGIASNIVSLVWLKRVFKKQSIA"
BASE COUNT          866 a          525 c          504 g          578 t
ORIGIN      
        1 ctaaaaaatt tgccattgca aatttttggt gcaccaaaaa tcctatggat tttttagtgt
       61 ttcaagcgtc aaaaaccact taaccgtcat gccaccagtc tatagcgact gaaaggagcg
      121 gtgactggtg gtttttgaca atgagtaaaa aatcggtaat tatttataac tgcatttcaa
      181 agatcagcct atgcctgagt atttagcatt tgataagtgc atcaagcttc taaaaaagca
      241 caagattgcc ctatctccct atttcattct tagctcaaaa gagcaagcaa tatcagtagc
      301 agcctctgtt ggatacccct tagttatcaa agcctatgcc gatggcatcg tacataaatt
      361 taaggaaggc tatgtcaaaa caggcatttc atcagaagat gagctgtcga aagaatatac
      421 atcaatatca aaaaaagcaa aagcaaggaa aaattgcgcg attgtcatcc aaaaagaggc
      481 gaaaggattt gagatgatct tgggtgctaa gctggatccc acctttggct atgtagtgct
      541 gcttggcctg ggtggaattt atactgaata tttaaagaag ttttcgatga gggtattgcc
      601 tataacaagc aaagacgcag ctgatatggt tgaagagatg cagataataa caggcaggat
      661 agccaagcaa aaggttgcca acttgatatt aagcatctcc aagatggcag taaaggaaaa
      721 gatgctcgaa ttagacctaa atccagtgat aataggcaaa gatgatatca atatagcaga
      781 tgtaaggatc ctaacatggc cctaaacgac cttttcaacc caaaaagcgt cgctgtcatc
      841 ggcgcatcta gagatacaaa aagcgcaggc tatggtatcc tcaaaaacct ggttgagggc
      901 agttttctca aatgcaggta tggaaagccg tttggaggaa aagtctatgc catcaaccct
      961 aacgctgatg aaattcttgg aaaaaattgt tttccatcta ttgctagaat aagcgaacct
     1021 atagacttag ctataattgc ccttccggtg aagatagtgg aaaaagaagt agataactgc
     1081 atcaaaaaga aggtaaaatc catcattctt atctctacag gttttgctga gataggcaag
     1141 gaaggaaagg ctatccagga caggattgca ggaaagctaa agaagcgtaa gatagacctt
     1201 cttggcccaa actgcctggg aataatcagg acaagcactc atctgaatgc aacatttgca
     1261 ccaagcatgc ctcctccagg aaaaattgca ttcatttccc aatcaggagc gctggcaaac
     1321 tctgtgatag actgggcaat agaagaaaga tatggtttta gctcaatagt aagcatcgga
     1381 aaccaggcag acaaagacat ccctgacttc atcgaatact ttgcaagaga tgaagagact
     1441 aaagtcatca cattatatat agagggattg aaagatggaa aaaaattctt aaatattgca
     1501 aagaaagcaa aaaaacccat aatagccatc aaatcaggaa aaactgcaca tggaaaaagc
     1561 gcggtttctt cccataccgg cagccttgca ggagattatg aagtctacca agcagccttc
     1621 aaacaagcag gagttatctt ggcagaaagt gtagaggaga tgtttgatat cgcaaaagct
     1681 cttgcctcac aacctattcc aacgaaaaat gccatagcca tagtcacaaa cggaggaggt
     1741 cctggagttc tttgcgcaga ctattgcagt gaaaatggag taaaccttat cactcttgaa
     1801 aaaaacacca taaaagagct ggaatcatca aacctcatgc atccagcatt ctcccgctca
     1861 aacccactag atatcgtagg cgatgctctt ccagaaagat acaagtttgc cataaatacc
     1921 cttctgtcaa aggactatat tgcaggcttg attataatcc agacactcca gaccatgaca
     1981 gacagcgcag agaatgcaag agcgatagtt gaggcatcaa aaaaataccc cgataagccg
     2041 ataatctgca cctatatcgg cggcaagttc agcaggcaaa gcataatcta cctcgaagca
     2101 aacagcgttc ctgactataa tgatgtaaga aaaagcgcaa aggttatggc agcgcttgtc
     2161 ggaaggatta agcgatagat tgtttcttaa acaccctctt caaccacacc aagctaacta
     2221 tattagaagc aatccccccc attatcatgg cgatagctat agaaagataa ccatatccca
     2281 tgacaaacac aaaaaagtat gccagcggaa tagccacaac aaacaccctg acagcattta
     2341 tcacaaatcc aggcaggccg tatcccatcg cctgcatcac cctgcttgct atcatggcaa
     2401 cagccatcgt tggaaatgtc agagcatcca gcatcatata aggcaccccc actgctatta
     2461 cagaagcatc aga
//
LOCUS       DASRUK010000092         4514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1590875, whole genome shotgun sequence.
ACCESSION   DASRUK010000092 DASRUK010000000
VERSION     DASRUK010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590875"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VFF28_03955"
     CDS             complement(<1..216)
                     /locus_tag="VFF28_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44809.1"
                     /translation="MAKTFFRVSYQMLMTFIGAYNPCNYFGYIEFLGESVLFSNGFQQ
                     MIVKYKDIQFIDQVSKYSVEVFFCKGTA"
     gene            291..2354
                     /locus_tag="VFF28_03960"
     CDS             291..2354
                     /locus_tag="VFF28_03960"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZX44810.1"
                     /translation="MIIKSVRLENIRSYLSETIDFPSGSTLLSGDIGSGKSTILLAIE
                     FALFGVKRADLSGSALLRHGKKSGSVELVLDIDGKEIIIKRALKRGKDDIKQEAGYII
                     SNGIKKEGTHIELKSQVLEMLGYPTDLLTKSKDLVFRYTVYTPQEAMKQILLEEKDAR
                     LDTLRKVFNIDKYKRIRENSEIFIRELKERRKNFEGKISDLELKLKQRELKEKEAVEM
                     QKQVDELLPKLETSKAELAKRKKDITQKEDTIKEYNRLKKEIELSELSLKNILERRKK
                     NQRDMEILSADMAKLKQDLDRMSISGIKSIASDLQKKANESRELSEMIISLNRSIAES
                     EARKKHSTETKQKIARIDQCPTCLQKVEESHKKRIHEKEDSVIKELELMISQCKEKER
                     AVKAKAEESAKEQEELRKKQSEIKVQSLKQESLKEKEQRNESLSKEQDTLKQEIGKIN
                     MKKLELGRRLDAVKDVESEYIALKRSYETALLEERKLEIGKRELEVQREHLLRYIQEL
                     ESEITEKEKYKLALARLAQFQNWLEKYFISLMNTMERHVMLQVYSEFNDLFRQWFSIL
                     IEDETMGVRLDDEFAPIIEQNGYETEIGNLSGGEKTAVALAYRLALNKVINDMIGTIK
                     TKDIIILDEPTDGFSTEQLDQVRIVLEQLSIKQVIIVSHESKIESFVDNVIRITKDEH
                     VSEIN"
     gene            complement(2351..3205)
                     /locus_tag="VFF28_03965"
     CDS             complement(2351..3205)
                     /locus_tag="VFF28_03965"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZX44811.1"
                     /translation="MAIDTHVHLMKYEKPTDYPWAKEMPYLQKDFSIDDLKQQLEGSG
                     IDGIVLVQSNESLAETDWLLQTAQNENLVKGVVGWFPVDGFRDIIARYKSNPRLVGAR
                     HIIQGQPAGFMDRENFNSALDNLAEFGLAYDLLVYQSQLAEANRLVTRHPQLTFVVDH
                     LAKPAIDVHRIHGRFISSYWNEEMKKLSEAPNVYVKFSGGVLEKDPRKWKGEIVNYYF
                     EMALRYFGSERIMYGSNAPLINDAMNCESGMKMWKEMAQKMVRLIHGIDLSKVMEGNA
                     RKAYNLKS"
     gene            complement(3210..4031)
                     /locus_tag="VFF28_03970"
     CDS             complement(3210..4031)
                     /locus_tag="VFF28_03970"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZX44812.1"
                     /translation="MDKGFLYTGLEGKAVIVTGGSQGIGAAIVRKFAELGSIVTILDL
                     NDDKGGEVVDSLNGHDIRFIHADLMAPDQCGRAVSEAALYGKQTTAMDGAYDRIDVLV
                     NCAGRNDNVSVGADRDKIMRSLELNVGQYAHMISLCWPYLISSKGNVINIGSKTGDVG
                     QKGSWAYSAAKGGVNALTYACARDGAPLDIRVNCVNPSEVPTELYHRWASDTFGGDAL
                     KAMAEIGAKIPLYGRMTSPEEIANAVVFLASNVLSSHTTGQILRPNGGHPQLNER"
     gene            4170..>4514
                     /locus_tag="VFF28_03975"
     CDS             4170..>4514
                     /locus_tag="VFF28_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44813.1"
                     /translation="MIRKVGRQGPKTLGISLPSKWIKKYSIVKGSELHITELHDQLLI
                     KRGKSTLQKQVSDVKIEGCDKRHIRLLINNAYRGGIDKLKVLFANPKVYDEVKHITTH
                     FLLGFEVTKKGQD"
BASE COUNT         1532 a          921 c          932 g         1129 t
ORIGIN      
        1 ggctgttccc ttgcagaaaa atacttctac agagtattta gatacttgat cgatgaattg
       61 aatgtcttta tacttaacaa tcatttgctg aaatccattt gaaaaaagca cactttcacc
      121 caaaaattcg atatatccaa aataattgca gggattataa gcgccgatga aggtcattag
      181 catttgatag ctgacgcgaa agaaagtctt tgccataatg ctttcccttg aattaatctt
      241 taaatctgct atgaaaagct atttatatct tcaagcattg cctgtattag atgataatca
      301 aatcggtcag gcttgaaaat atcaggagct atctttcaga gaccattgat tttccatctg
      361 gctcgaccct cttgtcagga gatatcggct ctggaaaatc aactatcctt ctagccatag
      421 aatttgcgct gtttggagta aaaagagcag atctatcggg ctcagccctg cttaggcatg
      481 gcaaaaaatc aggctctgtc gagcttgtgc ttgacattga cggaaaagag ataataataa
      541 agcgtgcctt aaagagaggg aaagacgata taaagcagga agcaggatat atcatatcaa
      601 acggtataaa aaaagaaggc acgcacatag agctaaagtc tcaagttctt gagatgcttg
      661 gctatcccac tgacctgctt acaaaaagca aagaccttgt tttcagatat acagtctata
      721 ctcctcagga agccatgaag cagatattgc tcgaagaaaa ggacgcccgc cttgatacct
      781 taaggaaagt ctttaacatc gacaaataca agcggataag ggaaaactca gagattttca
      841 taagagagct aaaggaaaga agaaaaaact ttgaggggaa gatttctgac cttgagctaa
      901 agctaaagca gcgtgaacta aaggagaaag aggctgtaga gatgcaaaag caggttgatg
      961 agctccttcc aaagcttgaa acctcaaaag cagagctggc aaagagaaaa aaggatataa
     1021 cccagaagga agatacgatc aaagagtata accggttaaa aaaagagata gagctaagtg
     1081 agctaagcct gaaaaatatc ctggaaagaa gaaagaagaa ccagagggat atggagattc
     1141 taagcgcaga catggcaaag ttgaagcaag accttgatcg aatgtcgata tcaggcatca
     1201 agagtatagc aagcgatctg caaaaaaagg caaatgaatc aagggagctt tcagagatga
     1261 ttatctcgct taaccgaagc atcgcagaat cagaggcaag aaaaaagcat agcaccgaga
     1321 caaagcaaaa gatagcaaga atagaccagt gccctacatg cctccaaaaa gtagaagaga
     1381 gccataaaaa aagaatccat gaaaaagagg actctgtgat aaaagagctg gagctaatga
     1441 tatctcagtg taaagaaaaa gagagggcag taaaggccaa agcagaagaa tcagcaaaag
     1501 agcaggaaga gttaagaaaa aagcagtcag agataaaagt acaatcgcta aagcaggaat
     1561 cgttgaagga aaaagagcaa agaaatgagt cgctgtcaaa agagcaggac actctaaagc
     1621 aggagatagg gaagataaac atgaaaaagc tcgagcttgg caggcgatta gatgcagtaa
     1681 aagacgtcga gagcgaatat atagctttga agcgttctta tgagacagcg ctgcttgaag
     1741 aaagaaagct ggagatagga aaacgtgagc tggaagtaca aagagagcat ctcttaagat
     1801 atatccaaga gctggaaagc gaaataacag aaaaggagaa gtacaagctt gccttggcaa
     1861 gacttgccca gttccaaaac tggcttgaaa aatattttat aagcctcatg aatacgatgg
     1921 aaaggcatgt catgctccag gtttactcgg aatttaacga cttgttcagg caatggttca
     1981 gcatacttat cgaagacgag accatgggtg taaggctcga tgatgagttt gctccaatta
     2041 tcgagcagaa cggctatgag acagaaatag gcaacctttc aggcggggaa aagacagcag
     2101 tagctttagc atacaggctt gccctaaaca aggtcatcaa cgatatgata ggtaccataa
     2161 agacaaaaga tataatcatt cttgacgagc cgaccgacgg tttttccaca gagcagttgg
     2221 accaggtgag gatagtgctt gaacagctaa gtataaaaca ggtgataata gtctcgcatg
     2281 aatcaaagat agaatcattc gttgacaacg tgataaggat aacgaaggat gagcatgtga
     2341 gtgagataaa ttaagacttg aggttatatg cttttctagc attcccttcc atgacctttg
     2401 acaaatcgat gccgtgaata agtctgacca tcttttgtgc catctccttc cacattttca
     2461 tcccgctctc acaattcata gcatcattta tcaggggagc atttgatccg tacataatcc
     2521 tttcagaccc aaaataacgc aatgccattt caaaataata atttactatt tcacctttcc
     2581 atttacgagg atccttctcc aaaactcctc ctgaaaactt aacgtaaaca ttgggcgctt
     2641 cagacaattt tttcatttct tcattccaat agctcgatat aaatctacca tgaattctat
     2701 gcacatcaat ggcaggcttg gcaagatggt caacgacaaa tgtcagttga ggatgccttg
     2761 taacgagccg gtttgcttct gcaagctggc tttgatagac caataagtca taagcaaggc
     2821 caaactctgc taaattatcg agcgcagagt taaaattttc tcgatccata aatcctgcgg
     2881 gctgtccttg gatgatatgg cgggcgccaa caagacgagg attactctta taacgggcga
     2941 tgatatccct aaatccgtcg actggaaacc agccgacaac accttttact aggttttcat
     3001 tttgtgcagt ctgaagcagc cagtccgtct ctgccaagga ttcgtttgac tgcaccaata
     3061 ctattccatc aattccgctt ccttctagtt gttgctttaa gtcatctatg ctgaaatcct
     3121 tttgcagata gggcatttct ttagcccatg gataatcagt aggcttttca tatttcatca
     3181 ggtgtacatg tgtgtctata gccatttttc tacctctcgt tcagttgcgg atgcccgcca
     3241 ttaggcctaa gtatttgccc tgtggtgtga gaagaaagaa cgttcgaagc aaggaataca
     3301 acggcatttg caatctcttc cggacttgtc attctaccat acaaaggtat ttttgcacca
     3361 atctcagcca tagcttttaa cgcatcacca ccaaaagtat cgcttgccca acggtgatag
     3421 agctcagtcg ggacctcgct tggattaacg caatttaccc ttatgtccaa gggagcacca
     3481 tctcttgcac aagcataggt caaggcattt acacccccct ttgcagcaga ataagcccat
     3541 gatccttttt ggccaacatc tcctgtttta gatccgatat ttataacatt gcccttgctg
     3601 ctgataagat atggccagca caggcttatc atatgtgcgt actgtccaac gtttaactcc
     3661 agagacctca taattttatc cctatccgcc cctaccgaaa cattatcgtt tcttccggca
     3721 cagttgacaa ggacatcaat cctatcatac gcgccatcca tggcggttgt ttgctttcca
     3781 tataaagcgg cttcactaac agctcgccca cattgatcag gagccattag atcagcatgt
     3841 ataaacctaa tatcatgccc attcaaagaa tcaacaactt cccctccctt gtcatcattt
     3901 aaatcaagga ttgtaacaat agaccctaac tctgcaaatt ttctgactat tgcagcgcca
     3961 atcccttgac tacctccagt aactatgacc gccttgcctt ctagcccagt atataaaaat
     4021 cccttatcca aatactatac cccctgtaaa aagtgtcatt cagcgcaaac tatgcaggcg
     4081 atatatattt tacctaccta ctacctatgt agatacaaaa atacctattt ttcaaaaagt
     4141 atatataagg tgacaaattc ccaagataca tgataagaaa ggttggccgg caagggccaa
     4201 aaacattagg aatatctttg cctagcaagt ggataaaaaa atattcgata gtaaaaggaa
     4261 gcgaattgca tatcacggag cttcatgacc aactgctaat aaaaaggggt aagtctactc
     4321 tacagaaaca ggtaagtgat gttaagattg aaggatgtga taaacggcat atcaggctat
     4381 tgataaacaa tgcatatagg ggcgggatcg acaaactcaa agttttattt gcaaatccaa
     4441 aagtgtatga tgaagtgaag catataacaa ctcattttct actcggtttt gaagtaacaa
     4501 aaaagggaca agac
//
LOCUS       DASRUK010000093         3557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_241766, whole genome shotgun sequence.
ACCESSION   DASRUK010000093 DASRUK010000000
VERSION     DASRUK010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241766"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VFF28_03980"
     CDS             complement(<1..438)
                     /locus_tag="VFF28_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44814.1"
                     /translation="MELQHSGVVSAVKRYIEEHKFDEKNTQLDTIFAHTKLEMNFGNV
                     VLHIKKTEHSLSALTELFKKLNLIGEDNVNLIMDKMSFALIFDNDNYQLIRGQFTSDK
                     IINELDNIGQVVLKFHPSVLKTPNVFATILNEVGINDINVIDSI"
     gene            complement(801..1349)
                     /locus_tag="VFF28_03985"
     CDS             complement(801..1349)
                     /locus_tag="VFF28_03985"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZX44815.1"
                     /translation="MASGLLTQAEYFILQPPAKAAMPDEHHKWIKSGMPYFDGRISGA
                     WQLYCSPLIEDDGIQMSMASQENERRRFACSPHYVAHINHGADKVVGSLWIDDIVEPN
                     VPRDSLARIHIRVDNHYRGQGIGSQLIRLFLDNHPAEYESAQCVWPDRGRPDDPTPLF
                     RRHGFQMAEQYGLNMAFIEFGK"
     gene            1428..2174
                     /locus_tag="VFF28_03990"
     CDS             1428..2174
                     /locus_tag="VFF28_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44816.1"
                     /translation="MPDKREQLREQLALLESLDIKTTRLKQLQEIADSVVDIADALGL
                     DETDADYKRLVGFVERYNAQLSQPDFGLPKRDYWGVLNESLIRVSPADSNLFLSRVYA
                     RKIKIGWRFGQEGRLALIDAMIFPENRVHNILTVDGYRSNDAWQTAPASCNAMPYETR
                     LLNPGFERLPLQAQYQSIARFGHSMAADAVRGLESGRIRAAGKLKDGDRQLTDIVGDQ
                     AGIYLLRKAGGLMPSVRMVGDNRLMIDYLG"
     gene            complement(2176..2847)
                     /locus_tag="VFF28_03995"
     CDS             complement(2176..2847)
                     /locus_tag="VFF28_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HZX44817.1"
                     /translation="MEFAYNGKHEYDDTDSTREFKQKLREWYSRPKHKPRQIKQYIVP
                     IILAFVIILLIIKLENNGSLERFSAKVNDTKENISKEIRMKTAEAKEAIQTKIEEYKE
                     DLEKREKDECQEAMAKVNDLRRKERIGKIQWNDDVYKIALERAKDMYERGYFSHDTPD
                     GKDIGDYIKNIGVSENIGKGYYSCSDAVDGWIESPGHHRNLLFGNFGAIAKSHDIYVY
                     VSTLP"
     gene            complement(2873..3064)
                     /locus_tag="VFF28_04000"
     CDS             complement(2873..3064)
                     /locus_tag="VFF28_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44818.1"
                     /translation="MKPITKSHNTFDILTYSILALVATTIGMTYNAPIIYLAAAVILT
                     LALFRRYWLHKRLDRGIKD"
     gene            complement(3069..>3557)
                     /locus_tag="VFF28_04005"
     CDS             complement(3069..>3557)
                     /locus_tag="VFF28_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013999221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HZX44819.1"
                     /translation="LKLLNDEIKVRFKDNKIKSKKFSERLEEAIRKYQNRSIDSAQVI
                     KELVEIAKEVREAQNRGEELGLTDEEVAFYDALVDNDSAVNILGQDVLKKIAVELTDL
                     IKKNTSIDWKLRATVRAKIRVMVKRLLGKYGYPPDKTAIAVDLVLEQAELLSEKWAVA
                     GA"
BASE COUNT          927 a          786 c          716 g         1128 t
ORIGIN      
        1 gattgagtcg ataacgttaa tgtcgttaat ccccacttca ttaaggattg tggcaaatac
       61 atttggtgtc ttaagcaccg agggatggaa tttaagtaca acctgcccga tattatctaa
      121 ctcattaatt atcttatcag atgtaaactg gcccctgata agctgataat tgtcattatc
      181 gaatattaag gcaaaggaca ttttgtccat gataaggttt acgttgtctt caccgattag
      241 gttcagcttc ttgaaaagct ctgtaagtgc acttaaagaa tgctctgtct tctttatatg
      301 gaggacaaca tttccaaaat tcatctccag ctttgtatgg gcaaagatgg tatccaactg
      361 ggtgttcttc tcgtcaaact tatgctcttc gatgtatctt tttacagcag aaacaacacc
      421 gctatgctgt agctccatct gagggttttg ccgcagtata aaatgagcta aagaatgaag
      481 gttaataacg tccctagcaa gatcccgctt aatgtcggta ttcctgtcaa ggaaattcca
      541 tacgtagttg gtgccgttga ccaatcatgc ccccgtcaca ctctttttta ctggaatctg
      601 acccttaggg atatgggaat gaaacggctt atttaaagct aacgctgaaa attggcattt
      661 tttgaggatg attttataag gattatataa atcagtcttt aagccttgcc caaccaatat
      721 tttctgaata aagcaaaagg ggttaaaaca gcggttgcaa aagaaacctt gcttgagagc
      781 atattattaa ctttatagag ttattttcca aattcgataa aggccatgtt caatccgtac
      841 tgttccgcca tctggaatcc atgtcttctg aaaagagggg ttggatcatc cggcctgcct
      901 ctgtcaggcc aaacacactg cgcgctttca tattccgccg gatggttgtc aagaaacaac
      961 cttatgagct gagagcctat tccctgccct ctataatggt tatctactct gatatggatt
     1021 cttgcaaggc tgtccctggg aacattaggc tcgacaatat catctatcca aaggctgccg
     1081 acaaccttat ctgcgccatg gttgatatgc gcaacataat gaggtgagca ggcaaatctc
     1141 ctcctctcat tctcctggga agccatagac atctgtatcc catcgtcttc tataagcggg
     1201 ctgcagtaaa gctgccatgc gccgcttatt cttccatcaa aatatggcat tcctgacttt
     1261 atccatttat ggtgttcatc aggcattgca gctttcgcag gaggttgaag tatgaaatat
     1321 tcagcttgag tcagtaatcc tgatgccata cttttatgta atttatataa tttttataaa
     1381 tcttacggag caataacctt tttattgatg ccagaattcc ttttcttatg cctgacaaaa
     1441 gagagcagtt aagggagcaa ttagcattgc ttgaaagcct tgacataaaa accacaaggt
     1501 tgaaacagct ccaggagata gctgattctg tagttgatat tgctgatgcc cttggccttg
     1561 atgagacaga tgccgactac aagaggcttg taggcttcgt agaaaggtat aatgcacagt
     1621 tgtcacagcc tgattttggt ctaccaaaga gagattattg gggggttctt aatgaaagct
     1681 taataagagt atctcctgcc gacagcaatc tcttcttatc cagggtttat gcaaggaaga
     1741 taaagattgg atggagattt ggccaggaag ggaggcttgc ccttatcgat gccatgatat
     1801 tccctgaaaa tagggttcat aatatcctga ctgtagacgg ctatcgctct aatgatgcct
     1861 ggcagacagc gccagcttcc tgtaatgcta tgccttatga aaccaggctc cttaatcctg
     1921 gctttgagag gcttcccctg caggcacaat atcagtcgat tgcaagattt ggccactcta
     1981 tggcagcaga tgctgttagg ggtttagagt cagggcgcat tagggcggca ggaaagctga
     2041 aagatggcga ccggcagcta acagatattg taggtgacca ggcagggatt tatctgctaa
     2101 gaaaagcagg aggtttgatg cccagtgtta ggatggttgg cgacaatcgg ttgatgattg
     2161 attatctagg atagtttacg gcaaagtcga cacataaaca tagatatcat gacttttagc
     2221 aatcgcccca aaattgccaa acagcaggtt tctatgatgc cctgggcttt ctatccagcc
     2281 gtcaacagca tcactgcagg agtagtagcc ctttccgatg ttttccgaaa caccgatatt
     2341 ttttatatag tcgccgatat cctttccgtc cggcgtatca tgtgagaagt agccccgttc
     2401 atacatgtcc tttgcccttt caagcgcaat cttatataca tcatcgttcc attggatctt
     2461 tccaatcctt tcttttcttc tcagatcatt aacctttgcc atggcctcct ggcattcatc
     2521 cttttccctt ttttccaaat cctctttgta ctcttcaatc tttgtctgga tagcctcttt
     2581 agcctctgca gtcttcatcc ttatctcttt gctgatattc tcctttgtgt cattgacttt
     2641 ggcactgaac ctttcaagcg agccgttgtt ttcaagctta ataatcagca ggataatgac
     2701 aaatgccaat attatgggca caatatattg cttgatttgt ctgggcttgt gttttggcct
     2761 tgaataccac tccctcagtt tttgtttaaa ctctctggtg ctgtctgtgt catcgtattc
     2821 atgcttgccg ttgtaggcaa attccatgtt tcctccagta tttactcttt ctctaatctt
     2881 ttatccctct atccagcctt ttatgaagcc aataccttct gaacaaggct agggtaagaa
     2941 tgactgcggc tgcaagataa atgatagggg cgttgtaggt catcccgatt gttgttgcaa
     3001 caagagcaag gatggaataa gttaggatgt caaaggtgtt gtgggatttg gtgatgggtt
     3061 tcaaaaaatt aggctcctgc aacagcccac ttctcagaca gcagctcagc ctgctcaagg
     3121 acaagatcaa ctgctattgc agtcttatca ggcgggtagc cgtattttcc cagaagcctc
     3181 tttaccatca ctcttatctt agccctgacc gttgccctta atttccaatc gattgaagta
     3241 ttctttttaa tgagatcagt aagttccaca gcgatttttt taagaacatc ctgcccaagt
     3301 atgtttactg cactatcatt atctacaagc gcatcataaa atgcaacctc ttcatcagta
     3361 aggcccagct cttcccctct gttctgtgct tcccttactt cttttgcaat ctcaacaagc
     3421 tcctttataa cctgtgctga atctatactc ctgttctggt attttctaat cgcttcttca
     3481 agcctttctg aaaatttttt actcttgatc ttattatctt taaacctgac ctttatttca
     3541 tcatttagca gctttaa
//
LOCUS       DASRUK010000094         6448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1257587, whole genome shotgun sequence.
ACCESSION   DASRUK010000094 DASRUK010000000
VERSION     DASRUK010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 6448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6448
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257587"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..2018
                     /locus_tag="VFF28_04010"
     CDS             237..2018
                     /locus_tag="VFF28_04010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZX44820.1"
                     /translation="MIKPEAELLFEASWEVCNKIGGIYTVVKSKAALTKKHYKNYFLV
                     GPYFEKNLVEFEQKEAPEYLSKSFSALEKEGIICKFGAWNIRGEPDMILVDSSRVVSK
                     KNEIKTALWNDYKIDSLYSQWEFEEPIVWSYAVARLIEEVSSRLKGKKIVAHFHEWLA
                     GTALLHLKKNKVNIGCVFTTHATMLGRTIAGFGEDLYGMLPKLNPDAEAYKRGIQDKF
                     TTERACAQNADVFTTVSEITAIEAEKILGRKPEVLLLNGLDIEKFPTVEEFSIKHVTC
                     REKIREFLISYFYPYYTFEVNNVTLMFIVGRYEFKNKGIDLFIHSLGQLNDRLKEEKS
                     KKTVAVFFWIPRENNGPKIEVLENKNYYRHIKNYVAFNSEKILSNIVYDIVSQKDISK
                     GHLFTKDFIQDIKKDILSFKRKGNPPIITHNINDEQNDIILKELINYGLDNREDDPVK
                     VIYYPTYLDVNDGLVNLSYYDAMAGCHLGVFPSYYEPWGYTPLEASALGVPSVTSDLA
                     GFGRFIEGKLKNNLNDGIFVLKRFNRKQEDIERDFTELLYKFIMRDKHSRAANKLNAK
                     ELSGLADWKLLIENYIKAHNLAVRKII"
     gene            2015..3736
                     /locus_tag="VFF28_04015"
     CDS             2015..3736
                     /locus_tag="VFF28_04015"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HZX44821.1"
                     /translation="MIVSHQLGRHVLIREGQAASAILSDNGSYLSIGLNSAYNGGFFM
                     LKGRLLKVVDEIGLPGKITRLTNKISSIEAVKEDILERFYLKKDALIYELSKPGWVRL
                     VLDVREPYDSRSWGKNYEISKIEDRLLIRYTKRADFRDGDKGTDEEYSLFIAIKSSHK
                     MDYRLVDDWLKKEYIFDKNRGSQHERYVYHAIDIFADKLVLSIAENKEDALSKACFFQ
                     SKRLNPIDSIKNKEILVAYNCAINSITGLTVEDNSICAGLPWFFQFWLRDESISVRSL
                     IDQGRLKTALNILDKQLSFIKDGSLPTIPGSSLPAMDALGWHFIGWQKLFTVLKGSDK
                     QKIIDKLEIALIDNLNNFSKDGLAANSSKETWMDSLDRSGARIEMQALRLSMHRFLAV
                     ITKKRYYSKLENNLRVKVRASFMKDNYLIDGIGDETVRPNIFIAAYVYPHLLTKKEWV
                     ACFEKVLPKLWLDWGGLATVDKESGDFHDAHTGQAPASYHNGDSWFWINNLAALVMYR
                     INKERFKPYIEKIIDASTGEILWKGFIGHHSELSSAKELKSEGCLCQAWSSAMYLELI
                     KEVYSLT"
     gene            complement(3729..3926)
                     /locus_tag="VFF28_04020"
     CDS             complement(3729..3926)
                     /locus_tag="VFF28_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44822.1"
                     /translation="MIKEVFVMSIACNICNGDFEKSPDSVVLCKFKEGMVHLGCCVNN
                     CSWDRKVCSNASAVYDKIDIK"
     gene            4018..6006
                     /locus_tag="VFF28_04025"
     CDS             4018..6006
                     /locus_tag="VFF28_04025"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008084258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HZX44823.1"
                     /translation="MIVEVMLVVSILSLAFAGYKSVLVSRSPAGNEKMKEISDAIHKG
                     AMTFLHKEYMILAVFVILVFAALYAFIDPKTAIAFVLGAIFSAIAGNVGMRIATKANT
                     RTAEGSKKSLNDGLNVAFSSGAVMGLTVVGLGLLGVTILYLIFDDPNIIYGFGFGASG
                     IALFARVGGGIYTKAADVGADLVGKVEVGIPEDDPRNPAVIADNVGDNVGDIAGMGAD
                     LFESYVDSIIAAMVIGSAILISTRVEKGYVILPMLLAGIGIFSSIIGTYFVRVTKGKA
                     QGALDKGIWAASIITAIISFFAIRFVIPGSSGLGIYYAMLAGLSSGVIIGLVTQYYTS
                     GDYRPTQKVAEAAQTGAGTNLISGLSVGMMSTLIPVLVISAAILVSYHFGGLYGIAIA
                     AVGMLSTLGITLATDCYGPVADNAAGIAEMAGLGGNIRKRCESLDAVGNTTAAIGKGF
                     AIGSAALTALALFASFAQLASGLTHQKMVIDVTSPTVIVGLFIGAVIPFLFSAMVMSA
                     VGRAAESVVIEVRRQFKTIKGLMQGKAKCDSSKCISIVTGSALKQMVIPSMIVVLTPI
                     IVGYFLGIQALGGVLVGATVTGFLLAVFMANAGATWDNAKKYVESGKFGGKGSQAHKA
                     AVVGDTVGDPFKDTAGPSLNILIKLMSIVALVFLPLFV"
BASE COUNT         1982 a         1254 c         1488 g         1724 t
ORIGIN      
        1 caaaggtagc gctgtttctt gcctgtcttg ttgatgcgaa aacctgcttt gacaaggttt
       61 gttgactttt tgcagaaaat gcaaaatacc tccataatat cacctcaaat gtcaatagaa
      121 cgaggtacta tattaatctg cagaagattt aacaatgcct ggtaaaaggt aaatttttta
      181 tcaataaatt tataaatagc tttgcgaaag aaacaatcaa aaaagaggta aatagattga
      241 taaagccaga agctgaattg ctgtttgaag cctcatggga ggtttgtaac aagattggcg
      301 gtatttatac cgttgtgaag agtaaagcag ccctcacaaa aaagcactat aaaaactatt
      361 ttcttgtcgg cccttacttc gaaaaaaacc ttgtagagtt tgagcaaaaa gaagcgcctg
      421 aatacttatc aaaatctttc tcagccttgg aaaaagaagg gataatctgt aagtttggcg
      481 cctggaatat aaggggggag ccggacatga tacttgtcga ttctagcagg gtagtaagca
      541 aaaaaaacga gataaagacc gctttatgga acgattataa aatagactca ttgtactctc
      601 aatgggagtt tgaagagccc atagtctggt cctatgcggt ggcgaggctt attgaggagg
      661 taagctcaag acttaaggga aagaagatag ttgcccattt ccatgaatgg cttgcaggaa
      721 cagctcttct tcacctcaag aaaaacaagg tcaatatagg ctgtgttttt acaacccacg
      781 cgacaatgct tggaaggacg attgcaggct tcggagaaga cctttatggc atgctgccta
      841 agttaaatcc tgatgcagaa gcctataaaa gaggcataca agataaattt accacggaaa
      901 gagcatgcgc tcaaaatgca gatgttttca caacagtaag tgagatcacc gctatcgaag
      961 cagaaaagat cctgggaaga aagccagaag tcttgctgct aaacggcctt gacattgaga
     1021 aattccccac agtcgaggag ttttcgataa agcatgtcac atgccgtgaa aagatcaggg
     1081 aatttttgat aagctatttc tacccgtatt acacttttga ggttaataat gttaccttga
     1141 tgtttatagt aggaaggtat gaatttaaaa ataaaggcat cgacctcttc atccactcac
     1201 tgggccagtt gaatgaccgg ttgaaagaag aaaagagcaa aaaaacagtc gctgtgttct
     1261 tctggatccc aagggaaaat aatgggccaa agatcgaggt attagagaac aaaaactatt
     1321 acaggcatat aaagaattat gttgccttca attccgaaaa gattctctca aatattgttt
     1381 atgatattgt ttcccagaag gatatttcca aaggccactt gtttaccaag gatttcatcc
     1441 aggacataaa gaaagatatt ttaagcttca agcgcaaagg aaatcctccc ataatcaccc
     1501 ataatatcaa tgatgagcag aacgatataa tcctaaaaga actgattaat tatggcctcg
     1561 ataacaggga agatgaccca gttaaggtaa tttactaccc tacctatctg gatgtaaacg
     1621 atggtcttgt aaatctctct tactacgacg ccatggctgg atgtcacctg ggagtctttc
     1681 cctcatatta tgagccctgg ggttatacgc ctctcgaagc atctgctctg ggtgtgccat
     1741 cagtaacatc agacctggca gggtttggca ggttcataga aggcaagctg aaaaacaatc
     1801 taaatgatgg aatattcgta ctaaaaaggt tcaaccgaaa acaggaagat attgaaagag
     1861 actttacaga acttttatac aaatttataa tgagggataa gcactcgagg gctgcaaaca
     1921 agcttaatgc aaaagagctt tcaggccttg cggattggaa attgctcatt gaaaactaca
     1981 taaaagccca taaccttgcc gtaaggaaaa tcatatgata gttagtcatc agctaggaag
     2041 gcacgttctt ataagagaag ggcaggcggc atcagccatt ctgtcggaca atggtagcta
     2101 tctctcgatt ggcctcaaca gcgcttataa tggaggcttc ttcatgctta agggtcgact
     2161 ccttaaagtg gtcgacgaga taggtttgcc tggaaaaatc actcgtttga caaacaagat
     2221 ctccagcatc gaagcagtca aagaagatat ccttgaacgt ttttacctaa aaaaagacgc
     2281 ccttatatat gaacttagca aacctgggtg ggtaaggctg gttcttgatg tcagagagcc
     2341 ttatgacagc agaagctggg gaaaaaacta cgagataagc aagatagagg ataggttgct
     2401 cataagatac acaaagaggg ctgactttag ggacggcgat aaaggcacgg acgaagaata
     2461 ctctttattc atagccataa aatccagcca taagatggat tataggttgg tggatgattg
     2521 gttaaaaaaa gaatacatct ttgacaaaaa cagaggttcg cagcatgagc gctatgttta
     2581 ccatgcgata gatatcttcg ccgataagct tgtcctaagt atagctgaaa acaaggaaga
     2641 tgccttaagc aaagcctgtt tttttcaaag caagcgatta aaccctatcg atagcataaa
     2701 gaacaaagag attttggtgg cctataactg cgcgataaac agtatcactg gcctcactgt
     2761 agaggataat agcatatgtg ctggcttgcc ttggtttttc cagttctggc taagagatga
     2821 atccatatca gtacgatcat tgattgacca gggaaggcta aagactgctc taaatatcct
     2881 agataagcag ctcagcttta taaaggacgg aagtctccct accattccag gaagtagttt
     2941 gcctgcaatg gatgcactcg gttggcactt cataggctgg cagaagctct ttacagtgct
     3001 gaagggaagt gataagcaaa agataataga caagcttgag atagcactta tagataacct
     3061 taacaatttc tcaaaagacg gtcttgcagc aaacagctca aaagagacct ggatggacag
     3121 ccttgataga agtggagcaa ggattgaaat gcaggcgcta aggctttcta tgcatcgttt
     3181 ccttgcagtc atcacaaaaa aaagatatta ctcaaagctt gaaaacaacc tgagggtaaa
     3241 agttagagcc tctttcatga aagataacta tctcatcgat ggtatcggcg atgagactgt
     3301 aaggccaaac atattcatcg cagcctatgt ctatcctcat ttgctgacaa agaaagaatg
     3361 ggtggcctgc tttgaaaaag tcctccctaa gctctggctt gattggggag gtcttgcgac
     3421 agtcgacaaa gaatctggtg atttccatga tgctcacaca ggacaggctc cagcaagcta
     3481 ccataatggc gattcatggt tctggataaa caatctggca gccctggtta tgtataggat
     3541 aaataaagaa agattcaagc cctatataga aaaaataatc gatgcctcaa caggcgagat
     3601 tctatggaaa ggctttatcg gccaccactc agagcttagc tctgcaaaag agcttaaaag
     3661 tgaaggctgc ctctgccagg catggagcag cgccatgtat ctggaattaa taaaagaagt
     3721 gtattccctt acttgatgtc tatcttgtca tatacagccg acgcattgct gcaaaccttt
     3781 ctgtcccagc tgcagttgtt aacgcagcat ccaagatgca ccatgccttc cttgaacttg
     3841 cacagcacca cagaatcagg gcttttttca aaatcaccat tgcatatatt gcatgcgata
     3901 ctcataacaa acacctcttt tatcataacc tcctggtaac taatatataa tgtttgcgtt
     3961 tggctgtaga aatcttttta tagatgaagc aagctatcag cagctgaggt ggtttaaatg
     4021 atagttgaag taatgttagt ggtaagtatc ctatctcttg cttttgcagg ctataagtcg
     4081 gttcttgttt caaggagccc tgctggaaat gagaagatga aagagatctc agatgctatc
     4141 cataaaggcg ctatgacctt tcttcacaag gagtatatga tcctggcagt ttttgtcatc
     4201 ctggtatttg cagctctcta tgcttttatt gacccaaaga ccgctatcgc ctttgttctc
     4261 ggagcgatct tttcagctat tgcaggaaat gtcggcatga ggatagcgac aaaagcaaac
     4321 acaagaacag cagaaggctc aaaaaagtca cttaatgatg gcttgaacgt cgctttctca
     4381 tcaggagcag tcatgggcct tactgttgtt ggccttggcc ttctcggagt aacgatactt
     4441 tacttgatat ttgatgatcc caatattatc tatggctttg gctttggcgc ttcggggata
     4501 gcattgttcg caagggttgg cggtggtatt tacaccaagg cagcagacgt aggcgctgac
     4561 cttgtaggaa aagttgaggt tggaattcca gaggatgacc caagaaaccc cgcagtcatc
     4621 gctgacaatg ttggagacaa tgtgggcgat atcgcaggca tgggcgcaga cttgtttgaa
     4681 agctatgttg attcgatcat tgctgctatg gtgataggtt cagcaatcct tatcagtaca
     4741 agggtggaga aaggatatgt catccttccg atgctccttg ccgggattgg gatattttct
     4801 tcaataattg ggacctattt tgtaagggtt acaaaaggaa aagcacaggg agcgcttgac
     4861 aaaggcatat gggcagcatc cataataact gcgatcatct catttttcgc cataaggttt
     4921 gtcatcccag gctcaagcgg ccttggaatt tattatgcta tgttagcagg gctttcatca
     4981 ggggtcatca tcggccttgt cactcaatat tatacatcag gcgactatag gcctacccaa
     5041 aaagtcgcag aggcagccca gaccggagca ggcacaaacc ttatctccgg cctttctgtc
     5101 gggatgatgt ctacacttat ccctgtgctt gtgatcagtg cagcaatcct tgtatcatac
     5161 cattttggag gattgtatgg catcgctatt gccgcagttg gcatgctttc aacactaggg
     5221 ataactcttg cgacagactg ctatggtcct gtcgcagata acgcagcagg aatcgcagag
     5281 atggccggtc ttggaggcaa tataagaaaa agatgcgaaa gccttgatgc agtaggaaac
     5341 actacagccg ctatcggaaa aggttttgcg ataggctcag ctgcacttac agctcttgct
     5401 ttgtttgctt cttttgctca actggcatcc gggctgacgc accaaaagat ggtcattgat
     5461 gttaccagtc cgactgtcat cgtaggcctg ttcattggcg cagtgatccc attcctgttt
     5521 tccgctatgg tgatgagcgc tgtaggaaga gcagcagagt cagttgtgat tgaagtaaga
     5581 aggcagttta agacgataaa aggcttgatg cagggaaaag caaaatgcga ttcaagcaaa
     5641 tgcatttcga tagtcacagg ctctgctcta aagcagatgg tcataccaag catgattgta
     5701 gtattgactc cgattatcgt gggatacttc ctgggaattc aggcgcttgg aggagttctt
     5761 gtcggagcga cagtgactgg ctttttgctt gcagtcttca tggcaaacgc aggggcaaca
     5821 tgggataatg caaagaagta tgtcgaatca ggcaagtttg gaggaaaagg ctctcaagcc
     5881 cataaagcag cagttgtcgg cgacacagta ggagacccat ttaaggacac agcaggccct
     5941 tcgctaaaca tcctgataaa gctgatgagt atagtggcgc ttgtgttttt gccgctattt
     6001 gtttgatttt tttcttttag attattgtac gataactcta acaaagccgc ggctcgccaa
     6061 atatgttttc ggtgatgaag tcgcgacaac ctgaaaggga gtattatagg atattaaaca
     6121 gggttggaaa tatcatgaac aaggagcttt atacattgat ggataatatt ggcagtggta
     6181 agtaggggat taaacttcaa tgtagcgatg gattatctga ggaagaacgc attgatagcg
     6241 aaattagggc tatttgattg gggtaagttt attagaaatg cattaactgc catagagcag
     6301 tgcattactc agaagttata acgcctcact ggctaattta ggaaaccccc tctccaagat
     6361 aagggtatag ttggatttaa caatccttaa ataacaattt aatttcccta ttcttatggt
     6421 caaagtagca gtcgttaaat gctctgat
//
LOCUS       DASRUK010000095         2241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1086262, whole genome shotgun sequence.
ACCESSION   DASRUK010000095 DASRUK010000000
VERSION     DASRUK010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086262"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="VFF28_04030"
     CDS             complement(<1..1226)
                     /locus_tag="VFF28_04030"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HZX44824.1"
                     /translation="MEYQRLVELYECLSKTTKRLAKTHLLSVFLSETTKEDIPRLVLL
                     LQGKVFPDWDERETGVASRLVMKAMSTATGISAEQIEQEWKTIGDLGEVALKVCSGKR
                     QSTLFSSELTIKKVFDNLRKLATLEGAGTVNNKMQLIAELLTSAKPLEAKYIVRTVLK
                     DLRVGVGEGSLRDAIVWAFFGDKVGLKYLDENKIDISSREEYNKYADAVQNAFNLTND
                     FASVAASAKEGLNSLLEMDIVVFRPLKVMLALKVDGVTEGFSRCGNPLDAEYKLDGFR
                     IQAHKKDGKVRLFTRRLEDVTVQFPEVVKYMLENVKAETFILDSEAAGYDTKTKKYLP
                     FQKISQRIKRKYDIEELSIKFPVEVNVFDLIYYNGKNMINEEFIHRREIIEKIVSKKP
                     GKIVPAKNLIASTEEEV"
     gene            1265..1909
                     /locus_tag="VFF28_04035"
     CDS             1265..1909
                     /locus_tag="VFF28_04035"
                     /inference="COORDINATES: protein motif:HMM:NF025865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZX44825.1"
                     /translation="MDVDTCIRERRSIRKFKDRPLDWEKITRIVDSARFAPSAGNIKN
                     YKLVVIRKDDARQKLASAAFDQDWMLDAPVHIVIMAEPDKAERFYGARGDRLYTVQNC
                     AAAIENMLLTASSLGLASCWVGAFDEEKVKHILNAPDGPEPQAIIVLGYADEKPEMPS
                     RMELEHQVYLDRWGGKGQGDKAKGYKSVAIKESIDNTKKTIKKFVSKIKKQVKE"
     gene            complement(1910..2035)
                     /locus_tag="VFF28_04040"
     CDS             complement(1910..2035)
                     /locus_tag="VFF28_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44826.1"
                     /translation="MGLAIGGAIYGLYRLCSGKKKEAPKEGAKPTAEVGQKEAPG"
BASE COUNT          659 a          512 c          445 g          625 t
ORIGIN      
        1 acctcttcct cagtggaagc gatcaagttc tttgcaggaa ctatctttcc aggttttttt
       61 gagactatct tctcgattat ctctctgcgg tgtatgaact cctcatttat catattcttt
      121 ccattataat atatcaggtc aaaaacattg acttcaacag gaaatttgat agaaagctct
      181 tcgatgtcgt acttccgttt tatcctttgg gaaattttct ggaaaggaag atacttcttt
      241 gtctttgtat cataaccagc agcctcagaa tcaaggataa aggtctcagc cttgacattt
      301 tcaagcatgt actttacaac ctcaggaaac tgcacagtta catcttcaag ccgccttgta
      361 aacagcctta ccttgccatc cttcttatgc gcctggatcc taaacccgtc cagcttatac
      421 tcagcatcaa gtggattgcc gcacctggaa aaaccctcag taacaccatc aaccttcaaa
      481 gcaagcatca ccttaagagg cctaaacaca acaatatcca tctcaagcaa tgaattaagc
      541 ccttcttttg ccgaagcagc aacagatgca aaatcattcg tcaggttaaa cgcattctgt
      601 acagcatcag cgtacttgtt gtactcctct ctgcttgaaa tatcgatctt gttctcatca
      661 agatacttca ggcccacttt atcaccaaaa aatgcccaga ctatagcatc tcgaaggctt
      721 ccttccccta ctcctactct aagatcctta agtaccgttc ttacaatata ctttgcctca
      781 agtggctttg ctgatgttag aagctcagcg ataagctgca tcttgttatt gacagtgcca
      841 gccccttcaa gagtagctag tttgcgaaga ttatcaaaaa cctttttgat agtaagctca
      901 gaagaaaaca aggttgactg cctcttgcca gagcatacct ttaaggcaac ttcaccaaga
      961 tcccctatag tcttccattc ctgctctatc tgttcagcag aaattccagt agcagtgctc
     1021 atggctttca taactagcct tgaagcaacc cctgtctctc gctcgtccca gtcaggaaag
     1081 acttttccct gtaagagtaa tactaaccgg gggatgtctt ccttggttgt ttctgaaagg
     1141 aaaactgaca gaagatgggt cttagccagc ctttttgtcg tctttgaaag gcattcatac
     1201 aattcgacaa gtctctgata ttccatcaaa aagattaaat attagctcgt ataaaagctt
     1261 tgttatggat gtagatacat gcataaggga gaggaggtcg ataagaaaat tcaaggaccg
     1321 cccgttggat tgggaaaaga taacacggat agtcgactcg gcaaggtttg ccccttcagc
     1381 aggtaatatc aagaactaca agctcgttgt catcagaaaa gatgatgcaa ggcaaaagct
     1441 tgcaagcgca gcattcgatc aggattggat gctcgatgct cctgtccata tagtcattat
     1501 ggcagagcct gacaaggcag agcgattcta tggcgcaagg ggagaccgcc tttacactgt
     1561 tcaaaactgc gcggcggcaa ttgaaaacat gcttcttact gcctcgtcgc ttggtcttgc
     1621 ctcatgttgg gtaggcgcat ttgatgaaga aaaggtaaag cacatcttaa acgcaccaga
     1681 tggccctgaa ccgcaggcga taatagtgtt aggctatgct gatgaaaagc ctgagatgcc
     1741 ttcaaggatg gagcttgagc accaggtcta tcttgaccgc tggggtggta aaggccaggg
     1801 agacaaggca aaaggttaca agtcggttgc tataaaagag tctattgaca atactaagaa
     1861 gacgataaag aagtttgtga gtaagattaa aaagcaagtt aaagaatgat tatcctggcg
     1921 cttctttctg acctacctct gcagtaggtt ttgctccctc ctttggcgct tcctttttct
     1981 tccctgaaca aaggcgatat aatccatata tagctccacc aatagcaagt cccataaggc
     2041 caccgactgc accgtaggca agaataggta gcctgcttgg agaaaaataa ctcagagtta
     2101 aaacctaatc tcgccatttg aataaattta atagggtcac gtttgtttta tatttttgtg
     2161 aaaaccataa aacaaaacgt aaccctaagc atcggtagtt tagcactaat agaaaaagtt
     2221 gacggtcaat atcaatattt t
//
LOCUS       DASRUK010000096        12632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2246812, whole genome shotgun sequence.
ACCESSION   DASRUK010000096 DASRUK010000000
VERSION     DASRUK010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 12632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12632
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2246812"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1381)
                     /gene="uvrC"
                     /locus_tag="VFF28_04045"
     CDS             complement(<1..1381)
                     /gene="uvrC"
                     /locus_tag="VFF28_04045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HZX44827.1"
                     /translation="MDLPHSPGCYLHKDRTGKIIYVGKAKDLFKRVSSYFNRNDLDAK
                     IAVLVAEIADTDFIVTDNEIEALVLENNLIKKHQPKYNINLKDAKRYAYIEITKEEFP
                     RLLVCRQRMKDGSYFGPFTSAATRDIVLSTLLKTLKFKRCKKMPKKACLRSHIDLCDA
                     PCAGNISATDYKRKVDAAEMVLSGKTDELLDKLKEELLIASKDERYEDALRLRDEISS
                     FEWLSEKQKVERQKKYNEDILNYLVSGGKVYLMLFNINKGCLENKQEFVFDYRDNFFD
                     EFILQYYSENEMPKEIIIPAAIDESLVRLLQRNKVKAVIPQKGEKRQLLELVLTNIKA
                     TFFSSQEKLVELQEKLKLPALPNVIECFDISHLSGTSTVGSMVQFREGRPDKNNYRRF
                     KIKTVEGVDDFECIAEVVRRRYSRLALENKPLPDLIMIDGGKGQLSSALAEMGKLGLN
                     VPMIALAKKE"
     gene            complement(1457..3205)
                     /locus_tag="VFF28_04050"
     CDS             complement(1457..3205)
                     /locus_tag="VFF28_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44828.1"
                     /translation="MSRLSALKEVLMRGQIPHGTVVRKNGQPRVPVIDDAVRRITEEL
                     GNYQPTSYARSMGNRKARLNPEQYEEVASQYADLVAALQDAMAILKALHRGRIEYSGK
                     TTQLPYSVWPKHQKAVPHSAKIVDLRSMIGERIDPYTRLYHRWQSANKAAKEEIADDI
                     SLLRQSIAKAQQSYDGLSTVYAALERGMTAPSDGWYHSAAGMLGCDKDLAVILTVLPK
                     REAAVAQSVRNIGPQSEEIRAKLATHLQNLSNPDEYVLTLDDIGAELGKGFQGQLEGA
                     LRDLSGWYQKPAGKATTLWDNGVTVGNNGNRALVAALQTSLNVEALLNGAANYESALN
                     RMVLDIGAGNFTGRYSLSFDPQQASESIATAFATIDGSQKLRKYYEGKVPQVGGLSRR
                     LGSAYIDGCEVLRQQSSGYLQMERVERFTLTSTKTGVIALIDQINAARRFSYGGIKPE
                     QVVDEDTYGRLREFATVLTEAEQKLSPSRRETYRSMSKQESEFQNAMSHTQDEGQTYG
                     WADNPLFRTAVVGCRQLADILPPQSDASQTTGMDRRVAHLFGSGAGSGGSIGDLLAKL
                     QRWETSIREYKTTYGL"
     gene            3414..3713
                     /locus_tag="VFF28_04055"
     CDS             3414..3713
                     /locus_tag="VFF28_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44829.1"
                     /translation="MKIKPSDRPKKRYIAFEVKDDCAINNTIKKSLTTVLGSNYPDAG
                     IKFIKDKYTKHRGIFRVNNRYSKLVVDALNKEGIKTVGMSGILKKAENKYLKVAS"
     gene            3729..4469
                     /gene="psmA"
                     /locus_tag="VFF28_04060"
     CDS             3729..4469
                     /gene="psmA"
                     /locus_tag="VFF28_04060"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014734541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit alpha"
                     /protein_id="HZX44830.1"
                     /translation="MVEPMPHQLMGYDRAITMFSPDGRLLQVEYAKKTVRQGSTAIGM
                     VCSDGVLFVTDKRIVDTLVVPESVEKIWQIDDHIGATASGILSDARVLIERAQLRAQQ
                     HRVTYDTPVDVVTIVKDIANLKQICTQSGGLRPFGVSILIAGIDGNKPTLFETDPTGI
                     FFEYRASVIGEGEVEVEEILQKEYKPDITIEDGFRLCLDALKKVLGENFSIDRIDAAY
                     IRKDERKFKKFTKDAIERNLGKKSLKKK"
     gene            4471..5172
                     /locus_tag="VFF28_04065"
     CDS             4471..5172
                     /locus_tag="VFF28_04065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly factor SBDS"
                     /protein_id="HZX44831.1"
                     /translation="MVSVDSANTIKLKTHGQSFEILADSTKALAIKHGTDMDIRDVLA
                     VQKVFTDSRKGLEASPNALKAVFGTDDPLEAAKLIIQKGDIPLTQEYKQGLRDQKKKQ
                     IIYMIHRNAVDPKTHLPHPPQRIEAALEEAKFHIDEFKEVTKQVEEALKAIRPILPIK
                     FETKEISAKIPPNYAAKSYSILHSFGKTLREDWQSDGSLVVIVEIPGGLEEEFHSKLN
                     ALCHGEVETKILNIR"
     gene            5178..5984
                     /gene="rrp4"
                     /locus_tag="VFF28_04070"
     CDS             5178..5984
                     /gene="rrp4"
                     /locus_tag="VFF28_04070"
                     /inference="COORDINATES: protein motif:HMM:NF003181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000178 - exosome (RNase complex)
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex RNA-binding protein Rrp4"
                     /protein_id="HZX44832.1"
                     /translation="MSNLLVKEKDVVVPGEILAEGMDYLPGPGTYREGNEIISGRLGL
                     TYIDGRAIKLIPLSGKYVPKRNDTIICKVIDISFSGWRVDTNSAYSAMLGLKDGSSSF
                     IQKGADLTQFYKVGDYIVAKVINVTSQKLIDITMKAPGLYKLKGGRIIEVNPNKVPRI
                     IGKMGSMVGMIKQATGCRITVGQNGLVWIEGDPDKELIVEKAIRKIEKESHLSGLTDS
                     IQRYLEKETGVKLVKEQAPPHIQHENAQQGEAQDNQYTQSEDDQGDNNEL"
     gene            5974..6693
                     /locus_tag="VFF28_04075"
     CDS             5974..6693
                     /locus_tag="VFF28_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204375.1"
                     /note="involved in the 3' to 5' degradation of a variety
                     of RNA species; forms a trimer of heterodimers (hexamer)
                     with Rrp42; Rrp41 is the catalytically active subunit;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex exonuclease Rrp41"
                     /protein_id="HZX44833.1"
                     /translation="MSYKKRPNNRKFEDTRPMEAKVGVIKRADGSAYFKFGNTWAYAA
                     VYGPRNLFPRFLQDPSKGILRCNYNMMPFSSRGERVRPGGSRRSKEISMVTEKALLPI
                     LDLDEYPNSVVDVFIELPETEAGSRTAGINAASMALADAGLLMKDLVCAVSVGRVDDK
                     IVVDLDYSEESYEDGPVADIPVAFLPKSDKLVLLQMDGLVSKEQLEKAISMGKDACRK
                     IYDVQKNALKEKYKSTGDSDE"
     gene            6686..7477
                     /gene="rrp42"
                     /locus_tag="VFF28_04080"
     CDS             6686..7477
                     /gene="rrp42"
                     /locus_tag="VFF28_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000178 - exosome (RNase complex)
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex protein Rrp42"
                     /protein_id="HZX44834.1"
                     /translation="MNEELKRHVVMLLTQDKRLDGRKSTDYRQPISIEPGFVGTAEGS
                     AKVTMGETEVIVGIKMEVGTPYPDRPDEGTIICGAELLPMSNPEFEAGPPGIQAVELA
                     RIVDRGIRESKTVDFKELCIKEGEKVWLILIDIVTLNDDGNLFDASALAAYAALKNAV
                     FPSFDGEKIDYSKKTDKKLKLRNVPLSVTICKIGERYLVDPTTEEEKVTDARLTVASI
                     EDGTICALQKGGEAPLSVDDVTKMIDIGIEKAKELRGVLNAVHKI"
     gene            7458..7694
                     /locus_tag="VFF28_04085"
     CDS             7458..7694
                     /locus_tag="VFF28_04085"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit K"
                     /protein_id="HZX44835.1"
                     /translation="MQYTKYEKARILGARSLQISMGAPFLIKLDDNDLKELNYNPIEI
                     AKKEFEKGLIPITVKRPYPSTENFDNENARKAKA"
     gene            7666..7947
                     /locus_tag="VFF28_04090"
     CDS             7666..7947
                     /locus_tag="VFF28_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF357 domain-containing protein"
                     /protein_id="HZX44836.1"
                     /translation="MKTLAKQKLEKYFSVARLAIKKAEESANRTSFISERADCLDMIK
                     RYFSDAEHFYSKDDYVNAFAALNYAHGWLDACARIGIFDVHDSKLFTVD"
     gene            8129..8419
                     /locus_tag="VFF28_04095"
     CDS             8129..8419
                     /locus_tag="VFF28_04095"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HZX44837.1"
                     /translation="MNDEDKLKQIRRHIDRIDTVIITALAERMSLMPEVAEIKKKNDI
                     TIFDERREVEIMKKLKKVAKEQGLDESFTEEIFLSCFNEAKRIQHEIINGSE"
     gene            complement(8422..9372)
                     /locus_tag="VFF28_04100"
     CDS             complement(8422..9372)
                     /locus_tag="VFF28_04100"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011012926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided pseudouridylation complex
                     pseudouridine synthase subunit Cbf5"
                     /protein_id="HZX44838.1"
                     /translation="MDLPFQTIKRELLVRRESVGNFGNSPSERTARDIINYGIVNIDK
                     PKGPTSHQVSDFVKKILNIGKAGHSGTLDPGVTGVLPVALGRATRIVQALLPAGKEYI
                     ALGYIHKPVSEQRIRQAFAELTGKITQKPPVKSAVRRVERTREIYYVDILEIDGQDVL
                     FRIGCQAGTYIRKYIHDLGLRLGCGAHMEELRRTKVASFDESTLATLQDLTDAYHFFR
                     QGNDKFIKKIVQPVENAVNHLPKVYILDSTIEPLSHGRDLAVPGISMVESGIRQQELV
                     AIMTLKGELVAMGPSALTSEEMVRNEKGIAVKTDKVFMHE"
     gene            complement(9404..9979)
                     /locus_tag="VFF28_04105"
     CDS             complement(9404..9979)
                     /locus_tag="VFF28_04105"
                     /inference="COORDINATES: protein motif:HMM:NF013371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44839.1"
                     /translation="MADKKASKIILERVYNVPLRRKYLRAPNWKRTKRAVTALQEFVW
                     QHMKPTELKIGKYANLELWKHGIKNPPHHIKVNCRKNEEGIVYVELVGAPVEKVKRKT
                     AKKAQEVKHEAIEVKTEPEALEAKSGLAEQKSEEVKQKKARRKKQAEEAEFEIDVKEA
                     LGGEEKKEEAPKVEEAKPKPAKQPKEAKAKE"
     gene            complement(9982..10155)
                     /locus_tag="VFF28_04110"
     CDS             complement(9982..10155)
                     /locus_tag="VFF28_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44840.1"
                     /translation="MSRFKHPSKKKRLSKLNRQTRWAPFWTVPKKYGKGRRVHPGRHT
                     YVKRNWRRTKTKA"
     gene            complement(10158..10493)
                     /locus_tag="VFF28_04115"
     CDS             complement(10158..10493)
                     /locus_tag="VFF28_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HZX44841.1"
                     /translation="MDELEELRKRRLAQLRRQQEEAWRKQADETEQVQQQIEQLEIAV
                     RQFLTKEALQRYGNLKAAHQEKAVQLLVVLGQAIQQGHIKEKITDDQLKQLLIKMEPE
                     RKEFKIKRI"
     gene            complement(10495..10959)
                     /locus_tag="VFF28_04120"
     CDS             complement(10495..10959)
                     /locus_tag="VFF28_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004592618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19e"
                     /protein_id="HZX44842.1"
                     /translation="MTIYDVNATELIEELAKELKKVDEIKAPEWAIYVKTGANKERPP
                     VRDDWWQVRAASVLRKVRLMGPIGVSKLRYYYGGKKNRGYKPERYMKGSGNILRKILQ
                     QLEKAELIKQGKKGVHKGRIATPKGIKLMDSAAKSLMKPVKKEEQKAPAAAE"
     gene            complement(11109..12341)
                     /locus_tag="VFF28_04125"
     CDS             complement(11109..12341)
                     /locus_tag="VFF28_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44843.1"
                     /translation="MKKGSLVLLVILMSLSVAAFPIKELGNCDSEKDCRNYCDEAKNL
                     LRCIAYAEEYGLMNKEEAELAKEMAGLIKEGKTPGGCSSEKACEDYCNIEENLIECVD
                     FAVRIGEISSDEAELVKQTNGKGPGDCRSREECDKYCEENMDKCIDYAVENNLLDERE
                     AELAKKTGGKGPGGCVDGECDEYCNKKENRQICLDFSVKYGLMTEGEANILSHCWDKK
                     ECDEFCKKSDEQFDACIDWLEKQGGIQEKELRSMKIMYYDSPGGCKGREECIESFCSD
                     DNEEHLFECEQYSVKLGLITEKQYEKWKEDYYEKKEESEKKDIDELKGREGSDKEVEL
                     EEDDDDLVEDDASDEEKSSDDGEGKTEEAEVDEDDSDEESDEANDESVSTGAVVVDYK
                     SKGYAWRFLEWIQDTFLS"
     gene            complement(12338..12541)
                     /locus_tag="VFF28_04130"
     CDS             complement(12338..12541)
                     /locus_tag="VFF28_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44844.1"
                     /translation="MDRKRLAIILIVAAAAVLTISIIVFVGTEDTYQKVDYTAKTAIV
                     DSFKTIEDANVFEKAKINPFEER"
BASE COUNT         3542 a         2832 c         2542 g         3716 t
ORIGIN      
        1 cttctttttt tgcaagcgcg atcataggaa cattcaatcc caattttccc atctcagcca
       61 aagcagaaga cagttgtcct ttgccgccgt ctatcattat caggtcaggc aggggtttat
      121 tctcaagggc aagccttgaa tacctccttc tcacaacttc agctatgcac tcaaaatcat
      181 caacaccctc aactgtctta atcttaaacc gcctataatt atttttatca ggccgcccct
      241 ccctgaattg aaccatcgag ccgacagttg acgtcccgga taagtgtgag atatcgaagc
      301 attctataac atttggcaag gcaggcagct ttaatttctc ctgcagctcg accaatttct
      361 cctgcgacga aaaaaatgtt gctttgatat tggtcaagac cagctcaagc agctgtcttt
      421 tctcgccttt ttgggggatt accgccttga ctttattcct ctgcagcaat ctaacgagcg
      481 actcatctat cgctgcaggg attatgatct ctttgggcat ctcattttca gagtagtatt
      541 gcaggataaa ctcatcaaag aaattatccc tgtagtcaaa aacaaattcc tgcttgttct
      601 cgaggcatcc tttattgata ttaaaaagca taaggtaaac cttgccgccg gataccaggt
      661 aattgaggat gtcttcatta tacttctttt gccgctcaac cttctgtttt tcactcagcc
      721 actcaaaaga gcttatctca tcccttagcc ttaatgcatc ttcatatctc tcgtcttttg
      781 atgcaattaa cagctcttct ttgagcttgt cgagcagctc atcggtcttt ccagaaagca
      841 ccatctctgc agcatctact ttccgtttat agtcagttgc actgatattt cctgcacaag
      901 gagcgtcgca tagatcgatg tggcttctaa ggcatgcctt ctttggcatc ttcttgcatc
      961 tcttgaactt aagggtttta aggagcgtgg ataatactat atcccttgtt gcagcagaag
     1021 taaacggccc gaagtaactg ccgtccttca ttctctgcct gcaaactagg agtcttggaa
     1081 actcttcctt tgttatctcg atgtaagcgt atctttttgc atccttgagg ttgatattat
     1141 atttaggctg gtgttttttt ataaggttgt tttcaaggac aagggcctca atctcattgt
     1201 cagttacgat gaagtcagta tctgcaattt cagcgactaa cacagcgatc tttgcatcaa
     1261 ggtcgtttct gttaaaataa gacgagaccc ttttgaaaag atcctttgcc ttgccgacat
     1321 agattatctt tcctgttctg tccttgtgaa gatagcagcc tgggctatgc gggaggtcca
     1381 tgtaaataag ttaatggaga aaagatttaa agttaactga agcttataaa aattgaaaaa
     1441 agtaacccaa aaaggattat aacccgtagg ttgtcttata ttcacggata cttgtctccc
     1501 atctttgcag ctttgccaac aagtcaccaa tgcttcctcc actacctgcg ccactaccga
     1561 aaagatgcgc taccctacgg tccatcccag tagtttggga tgcatctgat tgaggaggta
     1621 aaatatcagc tagctgtcta catcctacaa ctgctgttct aaataaggga ttatctgccc
     1681 atccatatgt ttgcccctca tcctgtgtat gactcatagc gttctggaat tcagactctt
     1741 gctttgacat agacctatat gtttctctcc tacttggact aagcttctgc tcggcttctg
     1801 taagtacagt cgcaaactcc cttaatcttc catatgtatc ttcatcaact acttgctcag
     1861 gttttattcc accgtacgag aacctccttg cggcattgat ttggtcaata agtgcgatga
     1921 cgcctgtttt tgtggaggtt aaagtaaatc tttctactct ttccatctgc aagtatcctg
     1981 agctttgttg ccttaaaacc tcgcatccat ctatatatgc actgcccagg cgtctggaaa
     2041 gaccacctac ctgcggtacc ttcccttcat aatatttcct caatttttga gagccatcga
     2101 tggttgcaaa cgctgtagct atagactcag aagcttgttg aggatcaaat gataagctat
     2161 accttcccgt aaaattacct gccccaatat caagaaccat gcggttcaac gctgattcat
     2221 aattagcagc cccgtttaat agcgcttcaa catttaacga agtctgtaat gctgcaacca
     2281 atgctctgtt tccattatta cctacggtta caccattgtc ccaaagcgtc gttgcttttc
     2341 cggcaggttt ctggtaccag ccgctaagat cccgtaaagc tccttccagt tgtccttgga
     2401 atcccttccc caactcagct ccaatatcgt ccaaggtaag aacatactca tcgggatttg
     2461 acaaattttg aagatgtgtt gccaatttag cccttatttc ttcactctga ggtccgatgt
     2521 ttctgacaga ttgtgctaca gcggcctctc tctttggtaa aacagttaat attacggcaa
     2581 ggtctttatc gcaacccagc attccagctg cggaatgata ccatccatca ctaggggctg
     2641 tcatacctct ctctaaagcg gcatagactg ttgataaacc atcataactt tgctgagctt
     2701 tggcaatcga ttgtcttaac agcgagatat catctgcaat ctcttctttt gccgctttat
     2761 ttgcactttg ccatctgtga tatagtcttg tatatggatc aatcctttcg cctatcatgc
     2821 ttcgcaaatc aacaattttg gcactatggg gtacggcttt ctgatgcttc ggccatacgc
     2881 tgtagggtaa ctgtgtagtt ttaccactat attcaattct tcctctatgt agggctttga
     2941 gaatagccat tgcatcttgc aatgccgcta ctagatcagc atattggctt gcaacttctt
     3001 catactgttc aggattaagt cttgcttttc tattacccat tgatcttgca taagaagttg
     3061 gttgataatt acctaattct tctgtaatac gtctaactgc atcatcaata acaggcactc
     3121 tcggctgtcc atttttccta acaactgtgc catgagggat ttgcccccgc attaaaactt
     3181 ccttaagtgc tgaaagtcga ctcatagtta ctacccctta ttgtaatact aagcgttttt
     3241 ctagaattct atataaagat ttatgtagta cgataccaat gttttattac gtaaagtagg
     3301 tagtaaaagt ttgcagacac taaaaccgat taatttataa acaaattcat tcttctatgc
     3361 tttgaagtga gagaatagcg gagaaagtat ttttttctca catattgtga aagatgaaga
     3421 taaaacctag cgatagaccg aaaaaaaggt acattgcatt tgaagtcaaa gacgactgtg
     3481 ctataaataa tactataaaa aaatccctca caaccgttct tggaagcaat taccctgacg
     3541 caggcataaa gtttataaaa gataaataca ccaagcacag ggggatattt agagtgaaca
     3601 acagatatag caaattagtt gttgacgccc tgaataagga aggtataaaa acagtgggta
     3661 tgagcgggat tctaaaaaaa gctgaaaaca aatacttaaa ggttgccagc taaaaaaagg
     3721 tgaaaaaaat ggtagaaccg atgccacatc aattgatggg atacgacaga gcgataacga
     3781 tgttttctcc tgacggaaga ctccttcagg tcgagtatgc aaagaaaaca gtaaggcagg
     3841 gcagcactgc aataggcatg gtatgctctg atggagtttt attcgttaca gacaaaagga
     3901 tagtcgacac attggtggtc ccagagagcg tggaaaaaat atggcagata gacgaccata
     3961 tcggggcaac agcatcaggt attctttcag atgcaagggt cctgatagaa agggctcaac
     4021 ttagggctca gcagcataga gttacatatg acaccccagt tgacgttgtt acaatagtta
     4081 aagacatcgc aaatctaaag cagatatgca cccaaagcgg cggccttagg ccatttggtg
     4141 tatccatctt gatagccggg atagacggca acaagccaac cttgttcgag acagacccaa
     4201 ccggcatctt ctttgaatat agagcaagtg ttataggaga aggcgaagtc gaggtagaag
     4261 agatcctcca gaaagaatac aagccagaca tcacaataga agatggtttt aggctatgcc
     4321 tggatgcatt aaaaaaagtc cttggcgaaa acttctctat cgacaggata gatgcagcat
     4381 acatcaggaa agatgaaagg aaattcaaaa agttcacgaa ggatgctata gaaagaaact
     4441 tgggaaaaaa atcattaaag aaaaaataaa atggtatcag tagacagtgc aaatacgatt
     4501 aagctaaaga cgcatggcca gagctttgag atactggcag atagcacaaa agctttagct
     4561 ataaagcatg gcactgacat ggatataagg gatgttcttg ctgtgcaaaa ggtattcact
     4621 gattccagaa aaggccttga ggcttctcca aatgcactaa aggcagtatt tggcacagac
     4681 gacccattgg aggcagcaaa actcatcatc caaaaaggag atatccctct aacccaggaa
     4741 tacaagcagg gcttaagaga ccaaaaaaag aagcagataa tatatatgat ccacagaaat
     4801 gcagtcgacc caaagacgca cttacctcat cctcctcaga ggatagaggc tgctttggaa
     4861 gaggccaagt ttcatatcga tgagtttaag gaagtaacta agcaagtgga agaggcatta
     4921 aaggcgataa ggccgatctt gcccataaag tttgagacaa aggagatatc agcaaagata
     4981 ccgccaaatt acgcagcaaa gtcatattcg attcttcatt cgtttggaaa gacattaaga
     5041 gaggactggc agagtgatgg cagcctcgtc gtcattgtcg agattccagg cggccttgaa
     5101 gaagagttcc atagcaagct taatgcttta tgccatggtg aagttgagac aaagatacta
     5161 aatataaggt gaaaacaatg agtaatttac tagtaaagga aaaagatgtt gtcgtcccag
     5221 gagagatcct tgcagaaggc atggactatc ttccagggcc aggaacatac agagaaggaa
     5281 atgagataat atcaggaagg cttggcctta catatataga cggaagagcg ataaagctta
     5341 tccctctttc gggaaaatac gtcccaaaaa ggaacgatac gataatatgc aaggtcatag
     5401 acataagctt cagcggatgg agggttgata caaactctgc atatagcgct atgcttggcc
     5461 taaaggatgg aagctcaagc tttatccaaa aaggcgcaga ccttacgcaa ttctacaagg
     5521 taggcgatta tatcgtggca aaggtcatca atgtcacaag ccagaagctt atagacataa
     5581 cgatgaaagc cccggggctt tataagctca agggcggaag gataatcgag gtaaatccca
     5641 acaaggtgcc aaggataatc ggcaagatgg gctctatggt aggtatgata aagcaagcca
     5701 ctggctgcag gataactgta ggtcaaaacg gcctagtgtg gatagaaggt gatccagata
     5761 aagagcttat cgttgaaaag gcgataagaa agatagagaa agaaagccac ctctcaggat
     5821 tgactgacag tattcagaga tacctggaaa aagagacagg agtaaagctg gtaaaagagc
     5881 aggctccccc tcacatccag cacgaaaacg ctcagcaagg tgaggcacaa gataaccaat
     5941 atactcaaag cgaagacgat caaggtgata ataatgagct ataagaagag accaaacaac
     6001 agaaaattcg aagacacaag accgatggag gcaaaagtcg gcgtcataaa aagagctgac
     6061 ggctcagcat atttcaagtt tggaaacaca tgggcatatg cagcagttta cggcccaaga
     6121 aacctgtttc caaggttcct ccaggatcct tcaaagggga tcttaaggtg caactacaac
     6181 atgatgcctt tttcctcaag gggagaaagg gtaaggccag gaggctcaag aagatcaaag
     6241 gaaatttcaa tggtaacaga aaaagcgctt ctgcctatcc tggacttgga cgaatatcca
     6301 aattcagtag tcgacgtgtt tatcgagctt cctgagacag aagctggctc aaggacagca
     6361 ggcataaacg ccgcttctat ggcacttgca gatgcaggcc ttctgatgaa agaccttgtc
     6421 tgcgcagtat ctgtaggaag ggtagatgat aagatagttg tcgacctgga ttattcagag
     6481 gagtcgtatg aagacggccc tgttgcagac atccctgtcg ctttcctgcc aaagtcagat
     6541 aagctggtcc ttcttcagat ggatggcctt gtttcaaaag aacagctgga aaaagcgata
     6601 agcatgggaa aagacgcttg caggaagatc tatgacgttc agaagaacgc attgaaggag
     6661 aaatataagt ctacaggtga tagcgatgaa tgaagagctt aagaggcatg tcgtgatgct
     6721 tttaacacag gataaaaggc tggacggaag aaagtccacc gattacaggc agcctatcag
     6781 catagagcca ggatttgttg gcacagctga gggttcagca aaggtcacta tgggcgagac
     6841 tgaagtgatt gtaggaataa agatggaagt cggaactccc taccctgaca ggcctgacga
     6901 ggggacgata atctgcggag cagagctttt acccatgtct aatccagaat ttgaagcagg
     6961 accgccagga atacaggctg tcgagcttgc gaggatagtg gataggggca taagggaaag
     7021 caagactgtc gattttaagg agctatgcat aaaggaagga gaaaaggtat ggctgatact
     7081 tatcgacatt gtcactctta atgatgatgg caatcttttt gatgcatctg cccttgccgc
     7141 ctatgcggca ttgaaaaatg cagttttccc atcatttgac ggagaaaaaa tagactactc
     7201 aaagaagact gacaaaaagc taaagttaag aaacgtgcct ttgtcagtta caatatgcaa
     7261 gattggagag agatatcttg tcgatccgac aactgaagag gagaaagtga cagatgcaag
     7321 gcttactgtg gcttctattg aagatggaac gatttgcgca ctccagaaag gtggagaagc
     7381 ccctttgtct gtcgatgatg tgacaaagat gatagacata ggcatagaga aagcaaagga
     7441 actcaggggt gtgttaaatg cagtacacaa aatatgagaa agcaaggatt ttgggcgcaa
     7501 ggtcgctcca gatctcgatg ggtgctcctt tccttatcaa gttggacgat aacgacctta
     7561 aggaactgaa ctataaccct atagagattg caaagaagga gtttgagaaa gggctgatcc
     7621 ctatcacggt caagcggcca tatccttcga ccgaaaactt tgataatgaa aacgctcgca
     7681 aagcaaaagc ttgagaagta tttttcagtg gcaaggttag cgataaagaa ggcagaagag
     7741 tccgctaacc gaacttcttt tatttctgaa agggcggatt gccttgatat gataaagcgt
     7801 tatttctctg atgcagagca tttctattca aaggatgatt acgtgaatgc ctttgcagcc
     7861 ttaaactatg cacacggatg gcttgatgcg tgtgcaagga tagggatatt tgatgtgcat
     7921 gatagtaagc tgtttacggt ggattgatta tttcattcat tagataactg ggattgtgca
     7981 ggggaaacat agtggcaaat ttctagagca atttggcgtc tatttcccct acgcataaaa
     8041 caaaaaataa tgatttaaat atcattggcc atcaaaaact ttttgtcaac aataaacttt
     8101 ataaatcacc tcaatctctg cgctaattat gaatgacgaa gataaattaa aacagattag
     8161 acggcacatc gacaggatag acacagtcat catcacagct ctcgcagaaa ggatgagcct
     8221 catgccagag gtcgctgaga taaagaagaa aaacgacata accatctttg acgaaagaag
     8281 agaagtggag ataatgaaga agctaaaaaa ggtagccaaa gagcagggtc ttgacgagag
     8341 cttcaccgaa gagattttcc tctcatgctt taacgaagca aaaaggatcc agcacgagat
     8401 aattaatggg tctgagtaaa cttattcatg cataaaaact ttatctgtct ttactgcaat
     8461 ccctttttcg tttctgacca tctcttcaga agtaagtgca gaaggcccca tggccaccaa
     8521 ttctcccttt aaggtcataa tcgcgacaag ttcttgctgc cttatccctg actcgaccat
     8581 gcttattccg ggaacagcaa ggtctcttcc atgcgacaac ggctctatcg ttgagtctaa
     8641 gatataaact ttcggcagat ggttgactgc gttctctact ggctgcacta ttttttttat
     8701 gaatttatca ttcccctgcc gaaaaaagtg ataagcatca gtaaggtcct gcaaggtagc
     8761 cagtgtggat tcgtcaaatg aagctacctt tgttcttctc agctcttcca tatgtgcccc
     8821 gcaaccaagt ctcaaaccca agtcatgtat atacttcctg atatacgttc ctgcctggca
     8881 tccaatcctg aacaggacgt cctgtccgtc aatctccagt atatccacgt agtagatctc
     8941 tcttgtcctt tcaacccttc ttactgcgct tttgactggc ggtttctgtg ttatctttcc
     9001 ggtaagttca gcaaatgcct gccttatcct ttgctcactg accggtttat ggatataacc
     9061 cagggcgata tactcttttc ctgcaggaag aagggcctgc acaattctgg tcgctcttcc
     9121 tagtgcaact ggcaatactc ctgtaacacc aggatccaaa gttcctgaat gcccagcttt
     9181 acctatatta agtatttttt tgacaaaatc actgacctga tgagaagtgg ggccttttgg
     9241 cttgtcgata ttgacgatgc catagttgat tatatctcta gcagtccttt cagaaggtga
     9301 gttgccaaag tttccgacag actctcttct gacgagaagc tctcttttta tcgtttgaaa
     9361 tggaagatcc atggattatc agaaataggg atatattaaa aacttattct tttgctttag
     9421 cttcttttgg ttgctttgct ggttttggct tggcttcttc aactttagga gcttcctcct
     9481 tcttctcttc gccgcccaaa gcttccttga catcaatctc aaactcagct tcttctgctt
     9541 gcttttttct tcttgctttc ttctgcttta cctcttctga cttctgttca gcaagtcctg
     9601 atttagcctc taatgcttcc ggctctgttt taacttcaat tgcttcatgt ttaacttcct
     9661 gagccttctt tgcagtcttc ctcttaactt tctcaacagg cgctcctaca agctcgacat
     9721 agacgatgcc ttcttcattc ttcctgcagt ttactttaat atgatgagga ggattcttta
     9781 tcccatgctt ccaaagctca agatttgcat atttacctat ttttaactct gtaggcttca
     9841 tatgctgcca aacgaattcc tgaagagcgg taactgccct ttttgttctc ttccagtttg
     9901 gagccctgag atatttcctt ctaagtggaa cattataaac gcgttcaagg attattttgc
     9961 ttgctttttt atctgccatt tttatgcttt agtctttgtc cttctccagt ttctcttgac
    10021 ataggtatgc cttcctggat gaacccttct tcctttgccg tatttctttg gcacagtcca
    10081 aaaaggagcc cacctagtct gcctgttaag cttgcttaac cttttctttt ttgaagggtg
    10141 tttgaacctt gacattttta tatccttttt atcttgaatt ctttcctttc aggctccatc
    10201 ttgatgagga gctgctttaa ttgatcatca gttatcttct cttttatgtg gccttgctgg
    10261 atagcttgtc ccaagaccac aagcagttgc acagcctttt cctgatgcgc tgctttaagg
    10321 tttccatacc tttgcagtgc ctccttggtc aaaaactgcc tgaccgcaat ttccagctgt
    10381 tctatctgct gctggacttg ctctgtctcg tctgcctgtt ttctccaggc ctcttcctgt
    10441 tgccttcgaa gctgtgctaa tcttcttttt ctgagttctt ccaactcatc cattttattc
    10501 agcagctgca ggtgcctttt gctcctcttt ctttacaggc ttcatcaatg acttagcagc
    10561 actatccatg agtttgatgc ctttaggcgt tgctattctt cctttatgca cacctttctt
    10621 tccctgcttg ataagctcag ctttctcaag ctgctgcagt atctttctta ggatatttcc
    10681 tgagcccttc atatatctct ctggtttata ccctctgttt ttcttaccgc cgtaataata
    10741 tcgtaattta gaaacaccta taggccccat caatctgacc tttctaagga cagatgcagc
    10801 acgaacctgc caccaatcat ccctgacagg aggcctttct ttgtttgctc cggttttcac
    10861 atagattgcc cattcaggag cttttatctc atccactttc tttagctctt tagcaagctc
    10921 ttctatgagt tccgttgcgt ttacatcgta tattgtcatt ttgtttcatg ccttgcggca
    10981 ttagaatggt tatatgaaat aaccaaatgc ttggataatg aacttattta taaacctttg
    11041 ggtttataaa tttttaaatg cctggcattt aaattattta taaatcattc ggtttaaaat
    11101 tataatgcct agctaaggaa agtatcctgt atccactcta aaaatctcca ggcataccct
    11161 tttgacttat agtcaacgac aactgctcct gttgaaactg attcatcatt agcctcgtca
    11221 ctttcttcat cgctgtcgtc ttcatctacc tctgcctcct ctgtttttcc ctcgccgtcg
    11281 tctgagcttt tctcttcatc tgatgcatca tcttccacta aatcatcgtc gtcttcctct
    11341 aattcgacct ctttatcact accttccctg cctttcagtt catcgatatc tttcttctcg
    11401 ctctcttcct tcttctcata ataatcttct ttccactttt cgtattgctt ctctgttatc
    11461 aagcctagct ttaccgagta ctgttcgcat tcaaacaagt gctcttcatt atcatcgctg
    11521 cagaagcttt cgatgcactc ttctcttcct ttgcatccgc cagggctgtc ataatacatt
    11581 atcttcatcg agcgaagctc tttttcctgt atcccgcctt gcttttcaag ccaatctata
    11641 caagcatcaa actgctcgtc gcttttcttg cagaattcat cacattcctt tttatcccag
    11701 cagtggctaa ggatatttgc ctcaccctct gtcatgagtc cgtatttcac agaaaaatca
    11761 aggcatatct gtcgattctc ttttttgttg caatactcgt cgcactcgcc gtcaacacat
    11821 ccgccagggc ctttgccgcc tgtcttcttg gcaagctctg cttccctctc atccaatagg
    11881 ttgttttcga cagcataatc gatgcattta tccatattct cttcgcaata tttgtcgcac
    11941 tcttccctgc tcctgcagtc tccaggaccc ttgccattcg tctgtttgac aagttcagcc
    12001 tcatctgagg atatctcccc aatcctcact gcaaaatcca cacactcaat aagattttcc
    12061 tcaatattgc aatagtcttc gcatgctttc tcagaactgc atccgccagg tgttttgcct
    12121 tctttgataa gccctgccat ctcttttgcg agttctgcct cctctttgtt cataagccca
    12181 tactcttcag cataagcaat gcatctcaat aaattttttg cctcatcgca ataattcctg
    12241 cagtcctttt ccgagtcgca gttcccaagc tcttttatgg gaaaggcagc cacgctgagt
    12301 gacatcaaaa tgacaagcaa tacgagtgat ccttttttca tctttcctcg aagggattga
    12361 tttttgcctt ttcaaacacg tttgcatcct ctatagtctt aaacgagtca acaatagcag
    12421 tcttggcagt gtaatcgacc ttctggtatg tatcttcagt gccaacaaac acgataatac
    12481 ttatcgtcaa cacagccgct gcagctacaa tcaggatgat agcaagcctt tttctgtcca
    12541 taaaacatca tttaaaaaaa gaagtttaaa tatttatcgc tggcggtgct atcgtcgtgt
    12601 gcggaaacta ggaatcggta aattctaatc tt
//
LOCUS       DASRUK010000097        11526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_83320, whole genome shotgun sequence.
ACCESSION   DASRUK010000097 DASRUK010000000
VERSION     DASRUK010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 11526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11526
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83320"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /gene="lgt"
                     /locus_tag="VFF28_04135"
     CDS             complement(<1..470)
                     /gene="lgt"
                     /locus_tag="VFF28_04135"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HZX44845.1"
                     /translation="MFYNNINPTFLKLGPFEIRYYGIIFVIGFIIAFFLIRYLAKKRE
                     LNLSKDDVWDLLFYIALGAVIGARLFEVIVYNFPYYLANPFEIIAVWHGGLSFHGGLI
                     GALSAGWLFCRKKKIGFYELADIVVVPLAFGLFLGRIANYINGELVGRATSVPW"
     gene            complement(436..1221)
                     /locus_tag="VFF28_04140"
     CDS             complement(436..1221)
                     /locus_tag="VFF28_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44846.1"
                     /translation="MNEWIKKRVEIFKSLDKAVLLQTLVIDMLFLAVVLFIGKYIGSL
                     IPADPVKLMEMFGTQGKLLLFAVGYPVVYYVFAIFLYSIAKLHILKMITPHRKIGMSE
                     LPKFFLLNILVFGGIFLIGLIVMMVLAVAFKQDFLKYIILVLGVPYLILAYTIFNLSH
                     SHFAKGDRQGLIRISLKDPFLHLGYLPYDLLFFAGFFIVFNVINVILRYTIFLNQGML
                     SSFGPVYIKIYNALFLIVIYAVVAFNRIFFVKNVLQQHKPDLS"
     gene            complement(1228..2397)
                     /locus_tag="VFF28_04145"
     CDS             complement(1228..2397)
                     /locus_tag="VFF28_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HZX44847.1"
                     /translation="MMKKLILFVLASLLLVSFASAENNVTIYFFYGDGCPHCAKAEPF
                     LDKLEAKYTELTVESFETWHNKTNADLFVDMSAACGSKVVGVPTVFIGHKPIVGFTTE
                     EETGKEIEGEVVKCIREGCVDLMERIGQNETSCPAEKPDSIITLPVFGELDVTKISLP
                     ALTIIIGLLDGFNPCAMWVLLFLLALLVYTKSRKRMIIIGGTFILVSGIVYYIFMAAW
                     LNFFLLIGYLPFLRAIVGGAAVIFGLINIKDMFWFKKGVSLSISDAAKPKLFERMRKI
                     VHESALPIALLSVTILAFTVNTIELLCTAGFPAIYTKILTMNDLSTFAYYGYLFLYIL
                     MYMLDDFLVFIIAVVTLSSKKLTEQQGKWMKFIAGVMMLGLGLLLIFKPELLMFR"
     gene            2666..3106
                     /locus_tag="VFF28_04150"
     CDS             2666..3106
                     /locus_tag="VFF28_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44848.1"
                     /translation="MKVMSSNIKIALSILIIAALAAFIVNINDLIPKEAPLLPTAGTD
                     NSGKDYSDCFGESPGTVLFVYSNSCPHCQNMKSIIKELEKEGYKFYWAEGSDPEARDI
                     IDQCFSDIIGGYVPQFICPANGQEKTGEMTKAELKNFADSCASS"
     gene            3456..4676
                     /locus_tag="VFF28_04155"
     CDS             3456..4676
                     /locus_tag="VFF28_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44849.1"
                     /translation="MEENKEAHEIRTAPGDEAHESEHEAHKTHHEAQHHETHHETHHH
                     AGEPMVHAQKIKKSSIWMAASAILAILLIASLVTGGFKGKSSSTGGDSFVFLNSEACT
                     TACSDIEPVAKEMAGKAGLAYSKAKYFQPVQVPGYLLVKDGVVTLNAVGTKANLASAL
                     CDATGSQAVCSEAQSAGTETQAEVEKSSTELCEAMPKVDKPKLEVFYVSRCPFGVQAI
                     NSLYYVKKNFGDKVDVIPRLLVEKSADGKSTTSMHGAEEHVEDLRHVCLREEQPAVYW
                     SYINCYAESGDGKTCEATAKVDSKKLEDCSKNRAESYALKDADDWAKIYRPKGGSGSP
                     SFFLNGEKINEYDFSQNGRSPDNVKTIVCCSAKTALKECESKLQTNQPPRGFGKMEAG
                     AAAAASSGQQLNCG"
     gene            complement(4696..5652)
                     /gene="thiL"
                     /locus_tag="VFF28_04160"
     CDS             complement(4696..5652)
                     /gene="thiL"
                     /locus_tag="VFF28_04160"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HZX44850.1"
                     /translation="MKLKDIGEFGLIDRIYRKPRDKNVLVGIGDDAAVVKMGGKLMLV
                     TTDTMVEGDHFSFDYFTPEQVGMKAIEVNVSDIGAMGGRPKYALVSLVLQNEMDVSVI
                     DGIYKGMRKSGDLHGVDIIGGNITHGKQLVIDIDMIGEVNNRELCLRGDAKPGDFILV
                     TGDLGASTAGLNLFLNKIKGHNEVKGKHVEPKAKLHKVRPFLGHINAMIDVSDGLASE
                     VGHICEQSGTGAVIYKDNIPIKDETRRAAEEVGKDPYDYALYGGEDFELVFTVSEKNL
                     RKVRGFLVGEITKRKGLFLYEKGKESRIKGNGYDHFKKKNKK"
     gene            complement(6228..7493)
                     /gene="thiC"
                     /locus_tag="VFF28_04165"
     CDS             complement(6228..7493)
                     /gene="thiC"
                     /locus_tag="VFF28_04165"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HZX44851.1"
                     /translation="MTQLESAKFNEITKTMEEVALKENIKVKELCNKIAQGRVVITAS
                     KLHKNLAPIGIGEGLKIKINANIGTSPNKVDIKEELEKLCVAIKYGADTIMDLSTGGE
                     IDSIRQQLISNCSVPLGTVPIYQAAIEKDSIVELSESDIIRVIRKQIQDGVDFITVHC
                     GIKKDVISLLDKRLTGVVSRGGSFIVKWMKHNNRENPFYENYDKILFWAKKEDVTLSL
                     GDGLRPGSLKDATDKAQLHELKILGELQKKAFAENVQSMIEGPGHVPLNQIEHNIKMQ
                     KQMCNNAPFYVLGPIVTDIAPGYDHITSAIGGTLAAYYGADYLCYVTPAEHLGLPTID
                     EVKEGVIASKIAAHAADIARGVKGARDWDDKMSEARANLDWPKMMELAIDREKAEATR
                     KRCQPDDPLVCSMCGKFCSMKIGKEMKES"
     gene            complement(7490..8110)
                     /gene="thiE"
                     /locus_tag="VFF28_04170"
     CDS             complement(7490..8110)
                     /gene="thiE"
                     /locus_tag="VFF28_04170"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HZX44852.1"
                     /translation="MKPSLRDINLYLVTDNTLTRKTIVEDVKDAVRAGVTIVQYREKD
                     KSTKEMVEEALLIKKICEGKAIFLVNDRIDVALAADADGVHLGQDDMPYQAARKILGG
                     KIIGLTAHNVLEAVSAEKLGADYVGVSPIFETATKKDAGIASGLRLIKEVKAATRIPI
                     VAIGGINLDNIDSVINSGADSAAVISAITTKEDVYGECKKFIERFK"
     gene            complement(8107..8901)
                     /gene="thiM"
                     /locus_tag="VFF28_04175"
     CDS             complement(8107..8901)
                     /gene="thiM"
                     /locus_tag="VFF28_04175"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HZX44853.1"
                     /translation="MNETAILLEKVRKQKPLVHHITNWVTIYDCANMTRAFGALPVMA
                     HAKEEAADMVKIAGALVLNIGTLTTELIESMIIAGKAANQKGIPVVLDAVGVGATKMR
                     DDKAAELLGAVHVDIIKGNGSEIARLAGESVQTKGVESTSVEGNLIEIAKKLATARKA
                     TVVITGAEDIITNGSETYLCKNGHKMMGSIVGTGCMAASIIGAFAAVEKDHTKAAAAA
                     LACFGIAGELAARNSNGPGSFKESFYDEAYNLNGNDIEDMEKLEKI"
     gene            9166..11028
                     /locus_tag="VFF28_04180"
     CDS             9166..11028
                     /locus_tag="VFF28_04180"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HZX44854.1"
                     /translation="MAFALNKEQAQAVNTTSGNLLIIASAGTGKTTTIVERYVNLVSN
                     HGFKPSEILMTTFTNKAAKDMIEKIRLKTSKAPEFIGTMHSLFLKMIRSHHALVLPAA
                     NFTIIDDSDKKRIIKQILIDMKIGPNADNVNYLMAGIGKFKNRGISSDYLDRGEAGKG
                     GREKEIINDEVLEISSALKAALPSVYKKYDSFLRSHNLIDLDDILLLALQLLNNHPEV
                     KEKYKIRFKAIMVDEAQDLNLVQMDILDLLHSNNLCLIGDDCQNIYEWRGSSNDLVFK
                     FNRHYPTIFLKNNYRSTKSIISAVNKTISSMKFRIDKELVCTRGKGTKISINSFYSQE
                     EEIAALASDVKELLKKGEKKEDIAVLFRTNTIGKAVEREFLRQRIPCHLSKSKGFFER
                     AEIKDILSFLKLKANNSSFPDFERVILLMEGVGKAKLIKIHALAKKHNCPVLEALGYI
                     SEVGFNEKLRQRLSSFKSLVSRDKLHPIKAFVSFFDYSNRIKADDNAKDKMENINALI
                     DMFSGYDYDAGGIVAFLDSLIEVEKKEKTKDKVALSTIHSSKGLEWKRVYLACCNDGL
                     LPYHNGNLTNVMRDSELRLFYVAISRAKDFLTVTYSGFNGWKEIYPSEFLEIIN"
     gene            11029..11232
                     /locus_tag="VFF28_04185"
     CDS             11029..11232
                     /locus_tag="VFF28_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44855.1"
                     /translation="MANQIDKTLINTLSKLAREEKRINGFRDRVRLKGKIKKKGKTKK
                     GNIRLKIEEGENVFNFVVIKTHK"
BASE COUNT         3480 a         2505 c         2288 g         3253 t
ORIGIN      
        1 caccaaggca ccgaagtggc ccttccgaca agctccccat tgatataatt ggctatcctg
       61 cccaaaaaaa gcccaaacgc aagaggtacg acaacaatat cagcaagctc ataaaaacct
      121 atttttttct ttctgcaaaa gagccatcca gcgctcaaag caccgattag tccgccatga
      181 aagctaagcc ccccatgcca cacagcgatt atctcaaaag gatttgctag ataataagga
      241 aaattataga cgataacctc aaaaagcctt gctccgatga cagctccaag ggcgatataa
      301 aaaagcaggt cccaaacatc atccttggaa aggtttagct ctcttttctt tgcaaggtat
      361 cttatcaaaa agaaagcgat gataaaccca attacaaaga tgatgccata ataccttatt
      421 tcaaagggcc caagcttaag aaaggtcggg tttatgttgt tgtaaaacat ttttgacaaa
      481 aaatatcctg ttaaaggcta ctacagcata gatgactatc aaaaagagag cattatagat
      541 tttgatatag acaggcccaa atgaagatag catcccttgg ttgagaaaga ttgtgtatct
      601 aagtataacg ttgatgacat taaaaacaat aaaaaatcca gcaaagaata ataagtcata
      661 aggtaagtac cccaggtgta gaaaaggatc ctttaagctt attctaatca gtccttgcct
      721 atcccccttt gcaaaatggc tgtggcttag gttaaagatt gtatacgcaa ggataagata
      781 aggcaccccc agcacaagga tgatatattt aaggaagtct tgcttgaatg caactgcaag
      841 aaccatcatg acaatgagcc ctataagaaa tatacctcca aataccaata tgttcagtaa
      901 aaagaactta ggcagttcac tcatacctat ttttctatga ggcgttatca tcttaaggat
      961 atggagtttg gcaatagaat acaaaaatat tgcaaacaca taatagacaa caggataccc
     1021 cactgcaaag agtagaagtt ttccttgcgt tccaaacatc tccatcaatt tcacagggtc
     1081 agccgggata agcgagccta tatattttcc aataaatagt accactgcca gaaatagcat
     1141 atctataact agcgtttgta atagcacagc cttatccaaa ctcttaaaga tctctaccct
     1201 cttttttatc cattcattca tcaagcatca cctaaacatc aatagctctg gcttaaatat
     1261 caataatagt ccgagcccaa gcatcatcac tccagcgata aacttcatcc acttcccctg
     1321 ctgctcagta agtttttttg agctcaaggt cactacagcg atgataaaca ccaaaaagtc
     1381 atcgagcata tacatcagga tgtaaaggaa aagataacca tagtaagcga atgtcgaaag
     1441 gtcgttcatc gtaagtatct tggtatagat tgcaggaaat cctgctgtac atagcagttc
     1501 tatggtattt actgtgaatg caaggatggt cacgctcaac aaagcaatgg gcagtgcaga
     1561 ctcatgcaca atctttctca tcctttcaaa caattttggc tttgcagcat cagagataga
     1621 aagcgatacc ccctttttga accagaacat gtcttttatg ttgatgagcc caaagatgac
     1681 agcagcgccg cccacgatag ctctaagaaa tggcaaatat ccgataagca ggaaaaaatt
     1741 aagccaggct gccatgaata tataatatac tatgcccgat acaagtatga atgttccgcc
     1801 gattattatc atccttttcc ttgatttggt atagacaagc agagcaagca agaataacag
     1861 tacccacata gcacagggat tgaatccatc cagcagtccg attatgatag ttaatgccgg
     1921 cagggatatt ttcgtgacgt ccaactctcc aaacacaggc agggtgatga tactatctgg
     1981 tttttcagca gggcatgagg tctcgttctg tcctatcctc tccatcaggt cgacacagcc
     2041 ttctctgatg catttgacga cttccccctc tatctccttt cctgtctctt cctcggttgt
     2101 aaatccgact attggtttat ggccgataaa gacagttggc actcccacta cctttgagcc
     2161 gcaggcagcg ctcatatcga caaagaggtc agcatttgtc ttattatgcc aagtttcaaa
     2221 agattctaca gtcagttcag tatacttagc ttcaagcttg tcaaggaagg gctctgcctt
     2281 tgcgcaatga gggcagccat caccataaaa gaaatagata gtgacattgt tctcagcgct
     2341 ggcaaagctt acgagcagaa gtgatgccag cacaaataat atgagtttct tcatcattat
     2401 ccttaggtgt aaaaaactac ttttataaat attgtgattt gttggaaatt accccactta
     2461 cttgcggtga ctgggttatt gttatggctc gccacgttga gggtatttac aacattaaaa
     2521 ataccctccc tccttggggc ggttgggatt tttatttgat gcctgatatc ttttacccct
     2581 ggccaaaaag agggattatc ctttttaacc gttccccagg agtcattcac aatgtttata
     2641 aataggtata gagaaaccta gctagatgaa agtgatgtcc agtaacataa agatagccct
     2701 ttctatcctc atcatcgctg cacttgctgc ctttatagta aatatcaatg acctgattcc
     2761 caaagaagcg ccgttgcttc cgacagcagg cacagataac tccggcaagg attactcaga
     2821 ttgttttgga gaaagcccag gtactgtttt gttcgtctat tcaaacagct gtcctcattg
     2881 ccagaatatg aaatccataa taaaagagtt ggaaaaggaa ggctataaat tttattgggc
     2941 agaaggttct gatccagagg caagggatat aatagaccag tgcttttcag atattattgg
     3001 gggttatgtt cctcagttta tctgtccggc aaacggtcag gagaagactg gtgagatgac
     3061 taaagcagag ctaaaaaact ttgccgatag ctgcgcaagc agttaaattt aaaaatgtca
     3121 aaaacattcc aaacaaatca tgctcagaaa tcgctcgatt ggtgttggca ccagatcgtt
     3181 caaagtgcaa aaggcgattt ctgacatgtc aaaaacttaa cattaatata taagcaggct
     3241 ataaggtttt tgagcatgct cagaaatcgc ttgcttggct aaggccttga ttgctcaaga
     3301 cttgggttca taccccgtag cttgctgcgg cttaataacc cagtttgagg cttaaaaaat
     3361 accccgcagc tcggctagcg gttgggattt tttatttaag atagcggttt taacatatca
     3421 aagtattcta tagggtactt tgggggtgta ttggtatgga agaaaataag gaagcgcatg
     3481 aaataaggac tgctcctgga gatgaggctc atgagagcga gcacgaagcg cataaaacgc
     3541 atcacgaagc gcagcatcac gaaacgcatc atgaaacgca tcatcacgca ggagagccca
     3601 tggttcatgc ccaaaagata aagaaatcat cgatatggat ggcagcaagc gcaattcttg
     3661 cgatattact cattgcttca ctagtgacag gaggctttaa gggaaaaagc agctccacag
     3721 gaggagatag ctttgttttc ctgaactcgg aagcatgcac tacagcttgc agtgatattg
     3781 agcctgtagc aaaagagatg gcaggaaaag ctggcttggc ctattccaaa gcaaaatatt
     3841 tccagccggt ccaggttcca ggttatcttc ttgttaaaga cggcgtggtc actttgaacg
     3901 ctgttgggac aaaggcaaat ctggcctcag ctctatgtga cgccacgggc agccaggcag
     3961 tgtgctcaga agcacagtca gcaggcacag agactcaggc agaagttgaa aagtcatcta
     4021 cagagctatg tgaagccatg ccaaaagttg ataagcctaa attggaagtg ttctatgtct
     4081 caagatgtcc gtttggtgtc caggcgatta actctttata ctatgtaaag aaaaacttcg
     4141 gcgataaagt ggatgtcata cccaggttgc ttgtagaaaa atcagccgat ggaaagtcca
     4201 ccacttccat gcatggtgca gaggagcacg tagaagactt aaggcatgtc tgtttaaggg
     4261 aagagcagcc ggcggtttat tggagttata ttaattgcta tgcagagtca ggcgacggca
     4321 agacttgtga agccacagca aaagtcgact caaagaagct tgaggattgc tccaaaaaca
     4381 gggcagaatc ttatgccttg aaagatgcag atgactgggc aaagatatac aggccaaagg
     4441 gaggcagtgg ctcgccaagt ttcttcctta atggagaaaa aattaatgag tacgatttca
     4501 gccagaatgg aaggtctcct gacaacgtca agacgattgt ttgctgcagc gcaaaaactg
     4561 ctttgaaaga atgcgaaagt aagctccaga caaaccagcc tccaagaggc tttggcaaga
     4621 tggaagcagg cgcagcagca gccgcaagct caggccagca gctcaactgc ggctaaattt
     4681 tttttgtttg ggactttatt ttttattctt tttcttgaaa tggtcgtatc catttccctt
     4741 gattctactt tccttgccct tctcatatag gaataatcct ttcctcttag ttatctctcc
     4801 gacaagaaat cctctgacct tccgtaggtt cttttcagaa acagtgaaca ctagctcaaa
     4861 atcttctccg ccatatagcg catagtcata tgggtctttg ccgacctctt cagcagccct
     4921 ccttgtctca tcttttatag gtatattatc cttatatatc acagcgcccg taccgctttg
     4981 ctcgcagata tggcctacct cagaggcaag tccatcgctg acgtctatca tggcattgat
     5041 atgccccaaa aaaggcctta ccttgtgaag ctttgctttt ggctcaacat gctttccctt
     5101 tacctcatta tgccccttta tcttgtttaa aaaaaggtta agccctgctg tcgatgctcc
     5161 aaggtcgcct gttacaagga taaagtctcc aggttttgca tcacctctta ggcatagctc
     5221 tctattattg acctcaccta tcatatctat atcgatgaca agttgttttc cgtgagttat
     5281 gtttccgcct attatgtcta ccccatgaag gtcaccagac ttcctcatcc ctttgtatat
     5341 tccatcgatt acagaaacat ccatctcgtt ttgtaggact aaagagacaa gagcatattt
     5401 tggcctgccg cccattgcgc cgatatcaga aacattgacc tctatagctt tcatccccac
     5461 ctgctcaggt gtaaaataat caaacgagaa atggtctcct tcgaccatag tatctgttgt
     5521 cacaagcatg agctttcctc ccatcttgac aacagcagca tcatcaccga tgccgacgag
     5581 cacattttta tcccttggtt ttctatagat cctgtcaata agcccaaact cacctatgtc
     5641 tttaagcttc atatgctcac cccagaacaa gcctgttaat gcgatatgga tatataaagc
     5701 ctttgaaacc taggcttttg tatttttagt tttgctaaaa tggctaaaag ctcaatattt
     5761 cgcctatatg gctcgttgag gacgtccaac ctcatttttc gcttgcagta atctataaca
     5821 acttaattgg tgaccaatta tccattgtaa tatgtgacca attatccatt gtaatatata
     5881 tctgtcaaat ctattgaatg acgcctctct tctggcatca gatacccttg aagtatgttg
     5941 gtaatctctg catatagttc cattacctac agcgctttgc aattggtggc caagttcata
     6001 atatgctaaa taagccttag cttgttcagg aagtgatccg ttgaatgatt caactaaatc
     6061 taaataagca ctatttgctt gaaactccaa attgtttagt gttctttttt cagctaactc
     6121 atcagccttt tcaattaggt ttataacatc gaataaaaac cttttgtccc ttttgcccta
     6181 aagcaccgat ttctgattga gtttaacaac tactaaccga acgtttacta agattccttc
     6241 atctccttcc cgatcttcat cgagcagaac ttcccgcaca tcgagcacac caaaggatca
     6301 tcaggctggc acctcttcct ggtcgcctca gctttctcac ggtcgattgc aagctccatc
     6361 atctttggcc agtcaaggtt agctcttgct tcagacatct tgtcatccca atctctggct
     6421 cctttaactc cacgtgcaat atcagcagca tgagcagcga tctttgaagc gatcacacct
     6481 tctttcactt catcgatagt aggaagcccc agatgttcag ctggcgtcac atagcataga
     6541 taatccgctc catagtaagc agcaagcgta ccgccgattg cagaagttat atgatcatat
     6601 cctggagcga tatcagtgac tattggccca agcacataaa atggtgcatt gttgcacatc
     6661 tgtttctgca tcttgatatt atgctctatc tgattcaaag gcacatgccc tggcccttct
     6721 atcattgatt ggacgttttc agcaaaagct ttcttttgaa gttccccaag aattttcagc
     6781 tcatgaagct gcgctttgtc agtcgcatcc ttaagcgacc caggtctaag cccatcaccg
     6841 aggctaagcg ttacatcttc ttttttagcc cagaacaata ttttgtcata attttcatag
     6901 aagggatttt cacggttgtt atgcttcatc catttgacga taaaagagcc gccgcgtgaa
     6961 acaacgcctg taagcctttt gtcaagcaat gagattacat ctttctttat cccgcagtgg
     7021 acagtgataa aatcaacccc atcctgtatc tgtttcctga ttaccctgat tatatcgctt
     7081 tcagaaagct cgactatcga atccttctcg atagcagcct gatatatagg cacagtccca
     7141 agcgggacag agcaattgct tatcagctgc tgcctgatag aatcaatctc tcctccagtg
     7201 ctaagatcca tgatagtatc agcgccgtac ttgatagcaa cacacagctt ttcaagttct
     7261 tccttgatat ccaccttatt cggtgaagtg ccgatatttg catttatctt tatcttaagt
     7321 ccttccccga ttcctatagg agcgagattt ttatgaagct tggaagcagt gatgactact
     7381 ctgccctgtg cgatcttgtt gcacagttct tttaccttta tattctcctt aagagcaacc
     7441 tcttccattg tttttgttat ttcattaaac tttgcagact caagctgtgt catttaaacc
     7501 tctctataaa tttcttgcac tccccataaa catcttcttt tgtggttatc gctgatatca
     7561 cagcagcaga atctgctccg gaatttataa cactatctat attatccagg ttgattccgc
     7621 cgatggcaac aataggtatg cgggtagcag ccttcacttc ttttatcagc ctaagccctg
     7681 aagctattcc tgcatctttc ttggttgcag tctcaaatat aggagagaca cctacataat
     7741 cagcgccaag cttttcagca gaaacagcct ctaaaacatt atgggcagta agaccgatga
     7801 tcttaccccc caggatcttt cttgcagctt gatacggcat atcgtcctgc cccagatgaa
     7861 caccgtccgc atcagcagca agcgcaacat ctatcctgtc gttcactaga aagattgcct
     7921 tgccctcaca tatctttttt attaaaagcg cctcttcgac catctccttg gtagatttat
     7981 ccttctccct atactgcact atcgttactc cagccctgac agcatccttc acatcttcaa
     8041 caatcgtttt tctggtaagt gtgttgtcag tgacaagata caggtttata tctctaagag
     8101 atggcttcat atcttctcta gcttctccat atcttctata tcattaccgt tcagattata
     8161 ggcttcatca tagaacgatt ctttaaagct tcctggtccg ttgctgtttc ttgccgcaag
     8221 ctctcctgca atgccaaagc aggctaaagc agcagccgca gcctttgtgt gatccttttc
     8281 aacagcagca aacgcaccga tgatagaagc agccatgcaa ccagtcccga cgatgcttcc
     8341 catcatctta tgcccatttt tgcacaggta agtctcactt ccatttgtga ttatatcttc
     8401 agcgccggtt atcacaacag ttgcctttct tgcagttgca agctttttgg caatctcgat
     8461 taagtttcct tcaaccgatg ttgactcaac accttttgtc tgcactgact cacctgcaag
     8521 ccttgcaatc tcagagccat ttccttttat tatatcaaca tgcactgccc caagcagttc
     8581 agcagcttta tcgtcgcgca tcttggttgc gccaacacca acagcatcca ggacaacagg
     8641 gatccctttt tgattagcag ccttgcctgc aattatcatc gattcgatca actcagttgt
     8701 aagagtccct atattcagaa caagagcgcc agcgatcttt accatatccg cagcctcttc
     8761 ttttgcatgc gccatgaccg gaagggcacc aaatgccctg gtcatattcg cgcaatcata
     8821 aattgttacc cagttagtga tatgatgcac taacggtttc tgtttcctaa ctttctccaa
     8881 taatattgct gtttcattca tttgtctacc tcacaatctg ttatcaattg taaacaatcc
     8941 atttcagcaa acataagaat atattaaaag gttatgcttc tgtctgcctt ttctgccggg
     9001 cgaatctttt ggatatcaaa aaatttatga agaaacagaa tctaagttaa agtgagggta
     9061 agtgattaga acgatggatt taggttcgtc tcgaaaaagg cgtcaaggaa ttgggcaggg
     9121 tggtaatgta ggctatacca taaaaaacat aatggtctca gggctatggc atttgcatta
     9181 aacaaagagc aagcacaggc agtaaacaca accagcggaa accttcttat tattgcaagc
     9241 gcaggcacag gaaaaacaac cacaatcgtt gaaagatatg taaacctggt gtctaatcat
     9301 ggcttcaagc cttctgaaat acttatgact acatttacta ataaagctgc aaaggatatg
     9361 attgaaaaaa taaggttaaa gaccagcaaa gcgcctgaat ttataggcac aatgcattct
     9421 ctttttctaa agatgattag gagtcaccat gctcttgttc ttccagctgc aaactttaca
     9481 ataatagatg actcggataa aaaaagaata ataaaacaga tactaattga tatgaaaata
     9541 ggtcccaatg cagacaatgt aaactatctg atggcaggca tagggaaatt taaaaatagg
     9601 ggcataagtt cagattatct tgacagagga gaagcaggca aaggaggcag ggagaaagag
     9661 ataataaatg atgaagttct ggaaataagc tctgctctta aggcagcact tccatcagtc
     9721 tataaaaagt atgattcctt cctgaggagc cataacttga ttgatttgga tgacattttg
     9781 ctgcttgctt tgcagcttct taacaaccat cctgaggtaa aagaaaaata taaaatcagg
     9841 ttcaaagcta tcatggtaga tgaggcccag gatcttaacc ttgtgcagat ggatatcctg
     9901 gatcttttgc atagtaataa cctttgccta ataggagatg actgtcaaaa tatatatgaa
     9961 tggagaggct catcaaatga tttagtattc aagtttaaca ggcattatcc tactattttt
    10021 ctaaaaaata actacaggtc cacaaagtcc ataatctctg ctgtaaacaa aacaataagc
    10081 tctatgaaat tcaggattga taaggagctt gtttgcacaa ggggcaaggg aacaaagatt
    10141 agcataaact cattctattc ccaggaagag gaaattgcag ctttggcgtc agatgtaaaa
    10201 gagcttttaa aaaaaggtga aaagaaggag gatattgctg tgcttttcag gacaaacacc
    10261 ataggaaaag ctgttgaaag ggaattttta aggcagagga ttccctgcca tctgtcaaaa
    10321 tcaaaagggt tttttgaaag ggcagaaata aaggacattt tatctttcct aaagcttaaa
    10381 gcaaacaaca gctctttccc tgattttgag agggttattt tattgatgga aggagttggg
    10441 aaagcaaagc tcataaaaat acatgccttg gcaaaaaagc ataattgccc ggttttagag
    10501 gctctgggct atatttctga agttggattt aatgaaaagc tcaggcagag gctaagcagc
    10561 tttaagtctc ttgtcagcag ggataagctg catccgatta aggcttttgt cagcttcttt
    10621 gattattcta acaggattaa ggctgatgac aatgccaaag ataagatgga aaatataaat
    10681 gccctgattg atatgttcag cggctatgat tatgatgctg gagggatagt tgcattttta
    10741 gacagcctga ttgaggttga aaaaaaggag aagacaaaag ataaggttgc tttaagcaca
    10801 atccacagct ccaaagggct tgagtggaaa agagtttacc ttgcctgttg caacgatggg
    10861 ctccttcctt atcataacgg aaatttaacc aatgtaatga gagacagcga gcttaggctg
    10921 ttttacgtgg caatatcaag ggctaaagat ttccttaccg taacctactc tggctttaat
    10981 ggctggaaag agatttatcc ctcagaattt ttggagatca ttaactaaat ggcaaatcaa
    11041 atagacaaga cccttatcaa cacgctttct aagttagcaa gggaggagaa aagaataaat
    11101 gggtttcgag atagggttag attaaaaggt aaaataaaaa agaaagggaa aacaaaaaaa
    11161 ggaaacatta ggctgaagat agaagaaggc gaaaatgtat tcaattttgt ggttatcaag
    11221 acacataaat agagatttgc acttgctgaa caattaaagg ttggctcgtt tgtgtctgct
    11281 gcgggaataa gaagatttag agctgtgatt tgcactcagt tgaagcagct tcataagatg
    11341 gatggcagca ggcaggagac gctttctgca aaaaactagt ccgaaactaa caagcacagt
    11401 ctaaggcacc attgaggtgg tcccaatggg aaaagttatt gcaaaaacag gaattaagag
    11461 agaaaaaggt tatctatgtt tcctgaacaa gaagggagat gttgccaggg tcaagatggc
    11521 aagagc
//
LOCUS       DASRUK010000098         4825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1425988, whole genome shotgun sequence.
ACCESSION   DASRUK010000098 DASRUK010000000
VERSION     DASRUK010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425988"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VFF28_04190"
     CDS             complement(<1..418)
                     /locus_tag="VFF28_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012954.4"
                     /note="FEN-1; Rad2; similar to eukaryotic enzymes;
                     endonuclease that cleave the 5'overhanging flap structure
                     that is generated by displacement synthesis when DNA
                     polymerase encounters the 5'end of a downstream Okazaki
                     fragment; as 5'endo-/exonuclease and
                     5'pseudo-Y-endonuclease activities; cleaves the junction
                     between single and double-stranded regions of flap DNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap structure-specific endonuclease"
                     /protein_id="HZX44856.1"
                     /translation="MGVAIGALLVKKEIELSSLSGKVLTVDAPMWLYQFLSSIRQPDG
                     TPLMDSKNNITSHLMGLSTRVPNLMEKGIKLAFCFDGKVPDLKQKERDRRNGLKLEAE
                     ALYEEAAKKQDIAGMKKYAQRSNRLTSNMITEAKELL"
     gene            complement(420..1397)
                     /locus_tag="VFF28_04195"
     CDS             complement(420..1397)
                     /locus_tag="VFF28_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44857.1"
                     /translation="MEDNREKILQYIRMKGPVLPADIGKQINSNILFASAHLSELSSN
                     GKVKVSSIKVGGSPLYYLPGQESMLQRFADDLGEKDKRAYNLLHEKKVLRDIELEPLM
                     RVALRGIKDFAIPLQVNLEGNSEIFWRWQEVTNQQAEELIRPLFVKEEKKIAEEKQEV
                     EKEEIKKVPEPEVKHQEKNQETNLEQKQEPRQELKPAPRQEIKKEIQSQISKDKPRPK
                     KEITDDGPFLDKISDHFKVNKIKIISKEQIRKSEFDLIIDVPSAVGNLEYYCKAKGKQ
                     KITDGDLASAFVQGQVKKLPVLLITNGELTKRAAEMLQKEFKGMSIAQI"
     gene            complement(1421..2086)
                     /locus_tag="VFF28_04200"
     CDS             complement(1421..2086)
                     /locus_tag="VFF28_04200"
                     /inference="COORDINATES: protein motif:HMM:NF019357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44858.1"
                     /translation="MKSPGKGLKKTVIREILINQYISNFSELSQKARKNLLKDLEVFG
                     NSILSSLVANKSKAIILPLNKKFTDLKEYSHLKGKPFYVKGYGKHDFYKTFDEVRGWG
                     GNPAVIGEEWVVAKDKHIFNVVQHEVAHQIQGVLPKHIQEKIHSAYLKAKKEGYIRKN
                     SSLCDGEYFAEAAAFYFNNSPSRKLQGYFDMDRIYNRKLLLKTDKILYSIIKEIFGNS
                     KNI"
     gene            complement(2061..2630)
                     /gene="endA"
                     /locus_tag="VFF28_04205"
     CDS             complement(2061..2630)
                     /gene="endA"
                     /locus_tag="VFF28_04205"
                     /EC_number="4.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000214 - tRNA-intron endonuclease
                     complex [Evidence IEA];
                     GO_function: GO:0000213 - tRNA-intron endonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-intron lyase"
                     /protein_id="HZX44859.1"
                     /translation="MSKKEKEGEIEEEKKPPVKSYLAEQRVMTESSDEARELYNKSRF
                     GTLLETGKVQMSLLEALYLLEKERLVVLDGKNKAVPFETFLKKAKKLEPNFWIRYCVF
                     KDIRNRGYIIKTALKFGADFRVYDRGVKPGEDHAKWIIYPVHEASTMTWYEFSAKNRV
                     AHSTRKRLLLAVVDDEGDCTYYEVAWKRP"
     gene            2836..3726
                     /gene="rnz"
                     /locus_tag="VFF28_04210"
     CDS             2836..3726
                     /gene="rnz"
                     /locus_tag="VFF28_04210"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HZX44860.1"
                     /translation="MEVTFLGTSSMVPTKDRNQSGIFIRYKNEGILVDCGEGMQRQFK
                     QKGIPLSKITKILISHWHGDHVFGLPGIISTLGSEEYQKTLEIYGPKGTAKRLNEMFD
                     AFVFDRKINLDVKEVSPGKFFESKDFYMEALPLEHGVDALGYSIVENDIRHIDVKKAK
                     KIGIPNGPLLGKLQDNETVVFKSKRIKPNDITFVEKGKKITVIMDTSPCNNAVKLASD
                     ADLVICEATYSSRLEEKGKEYGHMTGKQAGLLANQANAKKLVLTHFSARYKTTEEVEN
                     DAKDVFSNTTAAWDFMTIKI"
     gene            3734..4120
                     /gene="hjc"
                     /locus_tag="VFF28_04215"
     CDS             3734..4120
                     /gene="hjc"
                     /locus_tag="VFF28_04215"
                     /inference="COORDINATES: protein motif:HMM:NF040854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase Hjc"
                     /protein_id="HZX44861.1"
                     /translation="MSRKSKGINAERDLVHKFWANNWASVRIAGSGSMRYPSADIIAT
                     NKVRKLAIECKATKDTSKYIDKEDIDQLRQFAELFAAEPYIAIKFAKREWFFLTLEDM
                     EETDKSLMIDIEKAQIKGILFEELIK"
     gene            complement(4246..4446)
                     /locus_tag="VFF28_04220"
     CDS             complement(4246..4446)
                     /locus_tag="VFF28_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44862.1"
                     /translation="MALALGVQEVTLAIIVGTLFAIVYSLRVLVLMERRMARIEMHIE
                     SLVGNVMKEELKIEGKLSKKKR"
BASE COUNT         1349 a         1009 c          929 g         1538 t
ORIGIN      
        1 ccaaaagctc ttttgcctca gttatcatat tgcttgtaag cctgtttgac ctttgggcat
       61 attttttcat cccggctata tcctgctttt ttgctgcttc ttcatataat gcttctgctt
      121 cgagcttgag gccatttctt ctgtcccttt ctttctgttt taggtctgga actttgccgt
      181 caaagcagaa tgcaagcttt atgccttttt ccataaggtt tgggaccctt gttgaaagcc
      241 ccatcaaatg agaggttata ttatttttgg agtccataag aggcgtgcca tctggctggc
      301 ggatgctgct caagaattga taaagccaca taggagcatc gactgttagc acttttccag
      361 aaagagagct taactcaatc tcctttttta caagcaaagc acctattgct actcccatgt
      421 tagatctgtg ctatgctcat ccctttaaat tctttttgaa gcatctctgc tgcccttttg
      481 gtaagctcgc cgtttgtgat aagaagaacc gggagctttt ttacctggcc ctggacaaag
      541 gcgcttgcaa ggtctccatc agtaatcttt tgcttgcctt tggccttgca atagtattcc
      601 aggtttccga ctgcagatgg aacgtcaatt atcaaatcaa actctgattt tcttatctgc
      661 tcctttgaga tgatctttat cttgttaacc ttaaagtgat ctgaaatctt atcaaggaac
      721 ggcccatcat ctgtgatctc cttttttggc ctgggcttat ctttggatat ttgagattga
      781 atctcttttt ttatctcttg cctgggtgca ggttttagtt cttgccttgg ttcctgcttt
      841 tgctctagat ttgtctcctg atttttctcc tgatgcttaa cttctggctc aggaactttc
      901 ttgatctctt ctttctcaac ctcttgcttt tcttctgcta tttttttctc ttccttgaca
      961 aacaagggcc ttatcaattc ctctgcctgc tggtttgtaa cctcctgcca gcgccagaat
     1021 atctctgaat ttccctcaag attgacctga agagggatgg caaagtcttt tatgcctctg
     1081 agcgctaccc tcatcaaagg ctcaagctct atatctctta gaactttttt ttcatgaaga
     1141 agattgtacg cccttttgtc tttttcaccc agatcatctg caaacctttg cagcatcgac
     1201 tcctggcctg gaagatagta aagaggtgag ccgcctacct ttatgcttga gaccttgacc
     1261 ttgccgtttg aagaaagctc tgagagatgc gctgaggcaa aaaggatgtt agaattgatc
     1321 tgctttccta tgtctgcagg aaggactggg cctttcatcc ttatatactg caagatcttc
     1381 tctcggttat cttccatgcc tcaaagagaa agacttaagt ttatatattt ttagaatttc
     1441 caaatatttc tttaattata ctatacagta tcttgtccgt ttttaacagt aatttgcggt
     1501 tgtagatcct atccatgtca aaatatccct gtaactttct tgagggagag ttgttgaagt
     1561 aaaaggcagc tgcctcagca aagtactccc catcacataa ggatgagttt tttctgatat
     1621 aaccttcctt tttagctttg aggtaggcac tatgaatctt ctcttgtata tgttttggaa
     1681 gaactccctg aatctggtga gcaacctcat gttggactac attaaagata tgcttatcct
     1741 ttgccaccac ccattcctca cctatcactg caggatttcc tccccatccc cttacttcat
     1801 caaaggtttt gtaaaaatca tgtttaccat aacccttgac ataaaatggt tttcccttca
     1861 gatgagagta ttccttaaga tctgtgaatt tcttattcaa tggcaaaatg atagcctttg
     1921 atttatttgc cacaagtgaa gatagaatag agttgccaaa tacctccaag tccttaagca
     1981 aatttttcct ggctttttgg cttaattcgc taaagttaga aatatactgg tttatcaaaa
     2041 tttctctaat tactgttttt ttaaggcctt ttccaggcga cttcatagta tgtgcagtct
     2101 ccttcatcgt caacaactgc aaggagaagc ctttttcttg tcgagtgtgc aaccctgttt
     2161 tttgcagaaa actcatacca ggtcatggtg cttgcttcgt gaacaggata gataatccat
     2221 ttggcgtgat cctctccggg ttttacaccg cggtcatata ccctaaaatc agctccaaat
     2281 ttcaaggctg tcttgattat ataacctcta tttctaatat ccttgaaaac gcagtacctt
     2341 atccagaagt ttggctccag cttctttgct ttttttagaa aggtttcaaa gggaacagcc
     2401 ttgtttttgc cgtcaagtac aacaagcctc tctttttcca aaagatagag agcctcaagc
     2461 aaggacatct ggacctttcc tgtctcaagg agggtcccaa acctgctttt attgtataac
     2521 tcccttgcct catcagaact ttcagtcata actctttgct ctgcaagata ggattttaca
     2581 gggggctttt tttcttcttc tatttctcct tccttttcct tttttgacat aacctactat
     2641 tggtagtaat ttacttaaaa atgtttcttt ttaagctata gtttatggac cgttaacaac
     2701 aacatgggct tagagaccct tctctgctcg taaacccggc ggtctaaccc tgccttagcc
     2761 tcgcgggatt gttaacccga ttgactgcta tgtttccgac acttttaaat aattagggct
     2821 attttaaaga ggttaatgga agtaaccttc ttgggaacat catcaatggt tccgacaaaa
     2881 gatagaaacc agtcaggcat atttataaga tataagaatg aagggatact cgtagactgt
     2941 ggtgaaggga tgcagagaca gttcaaacaa aaaggtatcc ctctttcgaa gataactaaa
     3001 attctcatat cgcactggca tggagatcat gtgtttgggc ttccggggat aatatctaca
     3061 cttggctctg aagagtatca gaagacactt gagatatatg ggccaaaagg aacagcaaaa
     3121 aggctaaatg aaatgtttga tgcttttgta ttcgacagga aaataaattt agatgtcaag
     3181 gaagtcagtc ctgggaaatt ttttgagagc aaggattttt acatggaagc gttgcctctt
     3241 gagcatgggg ttgatgcgct tgggtattct atagttgaaa atgatataag gcatatagat
     3301 gtaaagaagg caaagaagat aggaataccc aatgggccgc tgcttggaaa gctccaggat
     3361 aatgagactg tcgtttttaa gagcaaaaga ataaaaccaa acgatatcac gtttgtcgaa
     3421 aaggggaaga agataacagt gataatggac acttcgccct gcaacaatgc agttaagctt
     3481 gcaagcgatg ctgacttagt catctgtgaa gcaacataca gctcaaggct tgaagaaaaa
     3541 ggaaaggaat acggccatat gaccggcaaa caggctggac ttcttgcaaa ccaggctaat
     3601 gcaaaaaagc tggtcttaac ccacttttct gccaggtata aaacaacaga ggaagtggaa
     3661 aacgatgcaa aggatgtgtt ttccaacacc accgctgcct gggatttcat gaccatcaaa
     3721 atttgaggct aatttgagca ggaaaagcaa aggcataaat gctgaaagag acctagtgca
     3781 taaattctgg gcaaacaatt gggcatctgt taggatcgct ggctctggct cgatgagata
     3841 tccttctgct gatataatcg caacaaacaa ggtgaggaag cttgctatcg aatgcaaagc
     3901 aacaaaagat acctcaaaat atatagataa agaagatata gatcagctaa gacaatttgc
     3961 agagctgttt gcggctgagc cttatatcgc gataaagttt gcaaaaaggg aatggttttt
     4021 tcttacgctg gaagatatgg aagagacaga caaaagcctt atgatagata tagaaaaagc
     4081 gcagataaaa ggtatattat ttgaagaatt aataaaataa aaatcccaac cgcctcaagg
     4141 ggcggggtat ttttagtgtt gtaaataccc tcaacgtggc gagccataac aataacccag
     4201 tcactgcaag cgtggggtat ttacaacaaa taaaaaaaca aaggattatc tcttcttttt
     4261 ggaaagcttg ccttctatct ttaattcctc tttcataaca tttccaacaa ggctttctat
     4321 gtgcatctca attcttgcca tccttctttc cataagaaca agaaccctta atgagtatac
     4381 tatagcaaaa agagtgccta caattatagc caatgttact tcctggactc ctagggctaa
     4441 agccatttta ccacctcttt ttttaatatg tcagaaatcg cttttgacgc atcctcttgt
     4501 gatctaaatc ccagagcagc gagcgatttc tgagtgtctg aaagctttgc tttctgacaa
     4561 accagaaatt tgctttgcaa atttctcagt tcatgctcaa aaactccgct ctacgattca
     4621 catttagtgc gggattcttg acatatatcg attattaaag aagtaatata taaatgttac
     4681 ttagcccaga cttccccgat gtagcagaat ttttcagtcc gcaatctcag catttcgaaa
     4741 aaatatcaga ttcataacaa aaattgacac ctgagcattt gcgctttttg gtgcaagagc
     4801 agcaattctg tattctggcg tctga
//
LOCUS       DASRUK010000099        12541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1426112, whole genome shotgun sequence.
ACCESSION   DASRUK010000099 DASRUK010000000
VERSION     DASRUK010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 12541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12541
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426112"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..2608
                     /gene="ppsA"
                     /locus_tag="VFF28_04225"
     CDS             200..2608
                     /gene="ppsA"
                     /locus_tag="VFF28_04225"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015224120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HZX44863.1"
                     /translation="MDKESKFVLWFDEIGITDVPLVGGKNASLGEMYQKLSSKGVNIP
                     NGFAITAYAYRYFLEEEHIKDELQSILKKVNPSSLESLEQAGHEARQQILKADFPPPL
                     KISIVEAYRQMCKKYGQHTDVAVRSSATAEDLPDASFAGQQETYLNIKGEYTLLDACK
                     KCFASLFTNRAIHYRIDKGFDHFKIALSIGVQKMVRSDLAASGVMFSIDTETGFKDAV
                     IINAIYGLGENIVQGQVNPDEYYVFKPTLKKGFKPIISKQIGSKDMRMVYCAGGSKHT
                     TKNVSVPEGERKKFVLCDEEVIKLAHWACTIEDHYGKPMDMEWAKDGHSNHLFIVQAR
                     PETVQSRKDVNVLEEYVLKKKGRIIANGKSVGARIGSGSAHVIINVHEIKKFSKGEIL
                     VTEMTDPDWEPIMKLASGIVTNKGGRTCHAAIISRELGIPCIVGTNDGTEKIRNGQAV
                     TVSCAEGEEGFVYEGSLPFEVKRHNLKNWKRPITKVMMNVGNPEQAFDFSFIPNDGVG
                     LAREEFIINEYIKVHPKALIYFDSIKDEETKAKILDLTSGYKDKKQFFVDKLAYGVAM
                     IAAAFYPKDVIVRMSDFKSNEYANLIGGKPFEPVEHNPMIGWRGASRYYTEYKDAFAL
                     ECKAMKKVRDEMGLNNVKLMIPFCRTVEEGKKVLKEMEKNGLKRGQNELEVYVMCEIP
                     SNVILADEFSKIFDGFSIGTNDLTQLTLGLDRDSELVSHVYDERNDAVKALVATVIKK
                     AKANKRKIGICGQSVSDYPEFAQFVVECGIDSISLNPDTVIKTTLTILKQEEKIKLDR
                     LRKS"
     gene            complement(2605..3615)
                     /locus_tag="VFF28_04230"
     CDS             complement(2605..3615)
                     /locus_tag="VFF28_04230"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HZX44864.1"
                     /translation="MRYLLQLSQDNLKLAEYEALSLLKPKKSRLEKNMLMIEVDDISL
                     SERLAYTKAVYELLFDCDINDAVGQVKKFVWEQIYESNFRVRVHNKGKLQEKEIASLI
                     WKSLKDPKVNLANPKTSLEFFIIGRKVYSTRLHVKLNHCFEERKAHKKPELHPTALSP
                     KLARALINLAGAQKSVMDPFCGAGGILVEAGLIGLKPIGYDLYNLMLKKAKANLDFFK
                     IRPYKLINKDALKIKERYDYVATDMPYGLNSSIWISKGKNNQRISVKENTKAEKKRRL
                     EDFYLKFLKNLRKVCKKKAVVIFPNYVSYRRIISESGFKKEKEFSQFVHGSLTRKIVV
                     LS"
     gene            complement(3612..4193)
                     /locus_tag="VFF28_04235"
     CDS             complement(3612..4193)
                     /locus_tag="VFF28_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44865.1"
                     /translation="MVDIRKIIIIFVIAVLFSVLVFSVIEAVYPSPKYDEICRQNIYP
                     EKPYITNRTCPDVNISVANRKACDEKKGYIEYTDYDENNCPTSYKCNTCQNEFNQAQQ
                     KHERVVFYISALLSLVAIFIGLYLPAESNSLNEWIGTGFMLGGTFSLFFGTLTTFIWL
                     DRYIRPIVILLELILVIFIAYRKIGNLRQDKKK"
     gene            4298..4615
                     /locus_tag="VFF28_04240"
     CDS             4298..4615
                     /locus_tag="VFF28_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44866.1"
                     /translation="MGRGRPVKSQIRQNLVELLFFLKEGYGYDLYRNYREIFSPVSMR
                     VIYYHLRKGADLGEFKVNKIIKESGNYSWGSEVEKIYYSLGEGAQPIGDDKVKEFLDR
                     KKV"
     gene            4646..5737
                     /gene="gcvT"
                     /locus_tag="VFF28_04245"
     CDS             4646..5737
                     /gene="gcvT"
                     /locus_tag="VFF28_04245"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017435175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HZX44867.1"
                     /translation="MALRTPLYSEHTALGARLVEFGGWDMPVCYSTITDEHNNVRKNA
                     GLFDICHMGEIFVEGNGSIDFLQKIITNDLNKLSSGKAFYTCMCNDSGGVLDDLFVYK
                     FNDDKFMMVVNAANIEKDFNWLASHSFGDVVLTNKSDETAKLDLQGPNSQQILQKLTQ
                     FDLSGMNRFSFAQDTVNDVDAIISRTGYTAEDGFEIYFEASKAAEIWRKLLDEGKEHG
                     LKPIGLGARDTLRTEACYSLYGHELNEEITPLEADVGFVVKLNKEFIGKGALLDAPKR
                     KVVAFEMKDKLIGRNGYEVFYGNKKIGSVTSGTFSPTFQKSIGMAMIDIDFARAGTVF
                     DIIIRDKMNTAEVVPKPIYKYVRNFSSSL"
     gene            5826..6029
                     /locus_tag="VFF28_04250"
     CDS             5826..6029
                     /locus_tag="VFF28_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44868.1"
                     /translation="MSEIASGARSDDLAQSKPAISEHAQEHAPTFRSAVLDTVEKLIN
                     EGRTNLLQWKIQKIYATAKSMNG"
     gene            5981..6358
                     /gene="gcvH"
                     /locus_tag="VFF28_04255"
     CDS             5981..6358
                     /gene="gcvH"
                     /locus_tag="VFF28_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HZX44869.1"
                     /translation="MENPKDLRYSKEHEWVKIENGVATIGITDFAQHQLTDIVFIELP
                     TAGKKAEQHKQIAVIESVKSVSDVFSPVSGDIVEVNSKLSDNPEIINKDPYGEGWIAK
                     INAKDAKELDNLLTADAYSKLIQ"
     gene            6402..7709
                     /gene="gcvPA"
                     /locus_tag="VFF28_04260"
     CDS             6402..7709
                     /gene="gcvPA"
                     /locus_tag="VFF28_04260"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HZX44870.1"
                     /translation="MEYICHTEEDIDKMLKAIKKKNISELFKDVPKKLLSSGLRVPQG
                     LSEIELKRLMNGLASKNKTELTYFLGAGAYKHFIPSVVNHILSKPEFYTAYTPYQPEM
                     SQGFLQAIYEYQSMICSITGMDASNASMYDGASALAESCIMACNITKRNKIIVSRAVH
                     PHYREVVRTYCKAHSIVLEEAEYENGVTSSVNVDRDTAALIIQSPNFFGGIEDIESFS
                     KMVHLNGALLNVCVVEPTSLGILKSPGKLGADIFIGEGQSFGNPVSFGGPYLGILATK
                     KEYVRQLPGRIVGKTVDTEGKEGFILTLQAREQHIRREKASSNICSNQALNMLAAAVY
                     LSYMGKSGFREAASQSAQKAHYLAKELEKVGVNPCFNSSFYNEFVVKVSDVKPVQERL
                     LSKGIVFGLLLGDYYPELNDCVLVCVTELNAIEEMDQVVEALK"
     gene            7706..9163
                     /gene="gcvPB"
                     /locus_tag="VFF28_04265"
     CDS             7706..9163
                     /gene="gcvPB"
                     /locus_tag="VFF28_04265"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HZX44871.1"
                     /translation="MRLIFELSKEGRMGASLPELDVPFKEGLIEDRFVSLDAELPEIS
                     EVDVVRHYTNLARRNFGVDNGFYPLGSCTMKYNPKVNEDISRFSGFTECHPLQPFSQG
                     SLELIYRLERLLCEICGMDAFTLQPAAGAHGELTGLMMIKAYFEDKGEARKKIITTDS
                     SHGTNPASASMCGFEVIQIKSNEDGLVDAALLDDALDKEVAAVMLTIPNTLGLFEKDI
                     VKIAKAVHEYGSLLYMDGANMNALLGTVRPGEMGVDVMHLNLHKTFSTPHGGGGPGSG
                     PVGVKKELAVFLPNPTVVSNDGAYEFSNNVKSIGRVRAFYGNFGVMVKAFSYIRALGR
                     DGLRRVSESAVLNANYIKEALKKDFKLPYDSLCKHEFVISDEGLPNHVTTADIAKRLI
                     DFGMHPMTIYFPLIVHGAMMIEPTETESKETLDNFILVMKQIRKEIDEDPSIVLSAPH
                     NTPVKRLDAVKAARELDLRYQYPKKAIVERVVPSR"
     gene            complement(9115..9459)
                     /locus_tag="VFF28_04270"
     CDS             complement(9115..9459)
                     /locus_tag="VFF28_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44872.1"
                     /translation="MGEESLEERIEKPRLWDFMVAAYSASRSIRTLLNSLALGYGYIA
                     SIAASSFFYLAAKTVGLTVKYAFLSLYYNIKDYSFLRYFRNSLNNYNPFGRLRRPHLL
                     GTTLSTIAFFGY"
     gene            9546..10256
                     /locus_tag="VFF28_04275"
     CDS             9546..10256
                     /locus_tag="VFF28_04275"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZX44873.1"
                     /translation="MIEQSLEKLGFSPSEIKVYLQLLKGCSYANKLSAETGINRTNVY
                     EALDRLIGKGVVSLITKNKVRWYDAKDANSLNSMIDERQGELAKLKADIAADIKDIAV
                     NEEKLEATIFTGKKGLRMLFDEMIEVGKPISIIASNHQFKEFFGPYFELWHKMRIERG
                     IRQRSIFPLKFEGKLEKRKLLTYKFVDDRYSNPTTTVIFGDNCLLIQWSNEPVAVKIF
                     NKEITRSHLNYFNMIYKK"
     gene            complement(10244..11422)
                     /locus_tag="VFF28_04280"
     CDS             complement(10244..11422)
                     /locus_tag="VFF28_04280"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZX44874.1"
                     /translation="MVDIQTIAALVFVIFMLVFLIINRKKLTIHKILLFPLLYFAMYK
                     TKIGLSWMDRAAKRFPRFIKTLGYIGVVLGFIGMVLITYALVDNMYSLFTKPSAVQGA
                     ALVLPIQAKGIFYVPFLYWIISIFIIAVIHEFSHGLLARAHNVKVKSSGFAFLGAVVP
                     IIPAAFVEPDEAQLNKKPIGPQLSVFAAGPFANIIAAFAFLGIMLLLTPFILNNIVTQ
                     EVMITGVAEGYGAEQAGITANESIISIDDQKINSRSELSKVLAEKKPGDIIELQTNRT
                     VYNLTLAENPGSKAQAYMGVNISEKFGINPSFEQKNGKTLASFILWLIGLINVLFFLN
                     LGIGLFNLAPIGPLDGGRMLLVTLKRYLKKELADKIFLYVSLALFLIIIIIVLSPYFL
                     "
     gene            complement(11533..11694)
                     /locus_tag="VFF28_04285"
     CDS             complement(11533..11694)
                     /locus_tag="VFF28_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44875.1"
                     /translation="MVKFKEAEKRKFANVFVCRNCKQKQRAPNLKVLAGKITCKRCGA
                     HTFRPVRRK"
     gene            complement(11694..12086)
                     /locus_tag="VFF28_04290"
     CDS             complement(11694..12086)
                     /locus_tag="VFF28_04290"
                     /inference="COORDINATES: protein motif:HMM:NF003076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-5A"
                     /protein_id="HZX44876.1"
                     /translation="MTTKPVDVGGLKKGSYVVLEGAACVVTDTQTSRPGKHGHAKVRL
                     TAVGIVDSKKRVAVMPGHDKLDAPVIEKKTAQVLSITGDMANIMDNESFETFDMQIPD
                     ELKEQVRSGSNVLYWVILNDKVMKQVQG"
     gene            complement(12095..12319)
                     /locus_tag="VFF28_04295"
     CDS             complement(12095..12319)
                     /locus_tag="VFF28_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010885767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone"
                     /protein_id="HZX44877.1"
                     /translation="MIFMSDKLLPLAAMEKIIKKVGGSDVRVSDKAKEALRTVMEEKA
                     EEIAEKAWRLATHAGRKTVKEEDIRLAVRQ"
BASE COUNT         3772 a         2372 c         2861 g         3536 t
ORIGIN      
        1 gtcttctcaa cagcttcgta accttccatc ttcatttgaa ttggttttct catgtagtta
       61 caatgtaact acaagaacta aataaatatt tcggtgctca agtatataat tagaattagt
      121 gggataaagc taaacgatta ccgaaaataa caagctttaa ataatgtttc tctggtaatt
      181 aggtaaaaaa gaggtactta tggataaaga gagcaagttt gttctgtggt ttgatgagat
      241 aggaataaca gatgttcctc tcgtcggcgg gaaaaatgct tcgcttgggg agatgtatca
      301 aaagctgtca agcaaagggg taaacatccc taacggattt gcgataactg catatgctta
      361 cagatatttt cttgaagaag agcacatcaa agatgagctt caatccatcc taaaaaaagt
      421 caatccatca agcctcgaga gccttgagca ggcaggccat gaggcaagac aacagatcct
      481 aaaggctgat tttcctcctc cacttaagat aagcatagtc gaagcttaca ggcagatgtg
      541 caagaagtat ggccagcaca ctgatgttgc ggtaagaagc agcgcaacgg ctgaggacct
      601 gccggatgcc tcttttgcag gccagcagga gacatacctc aacataaagg gagaatacac
      661 tcttcttgat gcatgcaaaa aatgctttgc atcacttttt acaaacagag ctatacatta
      721 tcgtattgac aaagggtttg atcatttcaa aatcgcttta agcataggag tccagaagat
      781 ggtaagaagc gatcttgctg caagcggagt aatgttctct atcgatacag agacaggatt
      841 taaggatgct gttataatca acgctatata tgggcttgga gaaaatatag tgcaagggca
      901 ggtaaatcct gatgagtatt atgtgttcaa accgacacta aaaaaagggt ttaaacctat
      961 aatcagcaaa cagatcggct caaaggatat gaggatggta tactgcgcag gcggcagcaa
     1021 acatacgacc aaaaatgtat ccgtgcctga gggcgaaagg aagaaatttg tgctttgcga
     1081 tgaagaagtg ataaagcttg cacactgggc atgcactatc gaagatcatt atggaaagcc
     1141 tatggacatg gagtgggcaa aagacggcca ttccaaccac ctgtttatcg tgcaggcaag
     1201 gcccgagaca gtgcagagca ggaaggatgt taatgttctt gaggagtatg tgttgaaaaa
     1261 gaagggcaga atcatagcaa atgggaaaag tgtgggggca aggataggaa gcgggagcgc
     1321 acacgttata ataaatgtac atgagataaa gaaattttca aaaggagaga tacttgtcac
     1381 tgagatgact gaccctgatt gggagcctat aatgaagctt gcgtctggaa tcgtgactaa
     1441 taaaggcgga aggacatgtc atgcagcaat aatctcaagg gaacttggga taccctgcat
     1501 tgtaggaacc aatgatggca ctgagaagat aagaaacggc caggcagtca ctgtgagctg
     1561 cgctgagggt gaagagggat ttgtgtatga aggaagcctg ccctttgaag tcaagaggca
     1621 taatctcaaa aactggaaaa ggcctataac aaaggttatg atgaatgtgg gcaatcctga
     1681 gcaggcattt gacttctcat ttatccctaa cgatggagta gggcttgcac gtgaagagtt
     1741 tatcatcaat gaatacatca aggtgcatcc taaggccctt atatactttg actctatcaa
     1801 agatgaagag acaaaggcaa aaatacttga tcttacatca ggatataaag acaagaagca
     1861 gttttttgtc gataagctcg catatggtgt tgcgatgatt gctgccgcgt tctaccctaa
     1921 ggatgtcatc gtcaggatga gtgattttaa atcaaacgaa tatgccaatc tgataggcgg
     1981 caagcctttt gagcctgtcg aacataatcc gatgatcgga tggagaggcg cttcaagata
     2041 ttatactgaa tataaagatg cttttgcgct agagtgcaag gcgatgaaaa aagtcaggga
     2101 tgagatgggc ctaaacaacg taaaactgat gatccctttc tgcaggactg ttgaagaagg
     2161 aaaaaaggtc ctgaaagaga tggagaagaa cgggcttaag cgagggcaga acgagcttga
     2221 ggtctatgtg atgtgcgaaa ttccatcaaa cgtgatactt gcagatgagt tttcaaagat
     2281 atttgacggg ttttcaatcg gaacaaatga ccttacgcag cttacacttg ggcttgatag
     2341 agactctgag cttgtcagcc atgtttatga tgaaaggaat gatgctgtca aagcgcttgt
     2401 cgctacggtc atcaaaaagg caaaggcgaa caagaggaag attggaatct gtgggcaatc
     2461 tgtctcagat taccctgaat ttgctcagtt tgttgtagag tgcggtattg acagcatttc
     2521 acttaatcct gatactgtga ttaagacgac tctaacaata ctaaaacaag aagaaaaaat
     2581 aaagttggat agactgagaa aaagttaact aagaacaaca atctttcttg tcagactgcc
     2641 atgcacaaat tgagagaact ctttttcttt cttaaagcct gactctgaaa tgatccttcg
     2701 gtagctaaca tagtttggaa atatcaccac tgcctttttt ttgcagactt tcctcaggtt
     2761 tttcaggaac ttaagataga agtcctcaag cctgcgcttt ttttctgcct tggtgttttc
     2821 tttgacactg atcctttgat tgttttttcc cttgctgatc caaatagagc tattgaggcc
     2881 atagggcatg tctgttgcca catagtcata tctttctttt atctttaagg catccttgtt
     2941 tatcagcttg taaggcctaa tcttaaagaa atccaggtta gcttttgctt tcttcagcat
     3001 caagttgtaa aggtcataac caatcggctt taatcctatc aggcctgctt ctaccaatat
     3061 tccacctgct ccgcagaatg ggtccataac acttttttgc gcccctgcaa gatttatcag
     3121 cgctcttgcg agctttggac taagcgctgt cgggtgaagt tcgggttttt tgtgggcttt
     3181 tctttcttca aagcagtggt ttagttttac atgcaatcta gtcgagtata ctttcctgcc
     3241 aatgataaaa aactcaagtg atgttttggg gtttgcaagg ttcaccttcg gatctttcaa
     3301 gcttttccaa atcaatgaag caatctcctt ttcctgcaat ttacccttat tatggactct
     3361 aacacggaaa tttgactcgt atatctgctc ccaaacaaac ttctttacct gcccaacagc
     3421 atcgtttata tcgcaatcaa agagcagctc gtagactgct ttagtataag cgagcctttc
     3481 tgaaaggctg atatcatcta cctcaatcat gagcatattt ttttcaagcc tgcttttttt
     3541 tggctttaac aatgacaagg cttcatattc tgcaagcttt agattatcct gcgataattg
     3601 aagaagatat ctcatttctt tttatcttgc ctaaggtttc caatctttct ataggcgatg
     3661 aagatgacaa ggataagctc aaggagaatg acaataggcc ttatatatcg gtcaagccag
     3721 ataaatgtag taagggtacc gaagaataaa gagaatgttc caccaagcat gaagcctgtg
     3781 cctatccatt cgttcaatga gtttgattct gccggaagat aaaggccgat aaatatggcg
     3841 acaagcgaga gcagggctga gatgtagaag acaacacgct cgtgcttttg ttgagcctgg
     3901 ttaaattcat tttggcatgt gttgcattta tagcttgtcg ggcagttatt ctcatcataa
     3961 tcagtatatt ctatataacc ttttttttca tcacaagcct ttctgttagc aacagatata
     4021 tttacatccg ggcaagtcct attggtgatg tatggctttt cagggtatat attctgcctg
     4081 caaatctcat catactttgg agaaggatag actgcttcta tcactgagaa aaccaatact
     4141 gagaacagaa cagcgatgac aaagattatg attatcttcc tgatatccac catgggttag
     4201 cctaacccgc gctaatttat aaaatttgtt gtttagtttt ttacttcaca aaaataatga
     4261 cagaagatat ataaatccct cagcgttcac ttgcagtatg ggaaggggga gacctgttaa
     4321 gtcacagata aggcaaaacc ttgttgaact cctgttcttt ttaaaagagg gttatggtta
     4381 tgacctgtat aggaactata gagagatatt ttctcctgtg agcatgaggg tcatctatta
     4441 tcatctcagg aagggcgctg acctcgggga attcaaggtc aacaaaataa tcaaggaaag
     4501 cgggaattac tcatggggct cagaggtaga gaagatatat tattcgcttg gagaaggggc
     4561 acaacctatc ggagacgata aagtcaagga gttcctggac aggaaaaagg tataattttt
     4621 ataattcttg tttttactga ggtttatggc ccttaggaca cccctataca gcgagcatac
     4681 agctcttggt gcaaggcttg ttgaattcgg cggctgggac atgcctgtct gctattctac
     4741 gataactgac gagcataata atgtccgtaa aaatgcaggg ctttttgata tctgccatat
     4801 gggggagata tttgtcgaag gtaatggctc tatcgatttc ttgcagaaga taatcaccaa
     4861 tgacttaaac aaactttctt ccggcaaggc attctatacc tgcatgtgca atgatagcgg
     4921 aggagtgctt gacgatcttt ttgtctataa attcaacgat gacaaattca tgatggtagt
     4981 caatgctgca aacatcgaga aagacttcaa ttggctagcc tcacattctt ttggggatgt
     5041 tgtgcttaca aataagtctg atgagacagc aaaacttgac ctgcaaggcc ctaactcgca
     5101 acaaatattg cagaagctta cccagtttga tctgtcaggg atgaatagat tttcttttgc
     5161 tcaggatact gttaatgatg ttgatgctat catatccaga accggatata ctgcagagga
     5221 tggattcgag atatattttg aagcttcaaa agctgcagag atctggcgaa aactgctcga
     5281 tgaaggcaaa gagcatgggt taaaacctat agggcttggc gcaagggata cactgcgaac
     5341 agaagcttgc tatagcctat atgggcatga gcttaatgaa gagataactc ctcttgaggc
     5401 tgatgtcgga tttgtcgtaa aattgaacaa agaatttatc ggcaagggtg cgcttcttga
     5461 tgcgccaaaa cgtaaagtgg tcgcttttga gatgaaggac aagcttatcg gaagaaatgg
     5521 ttatgaagtt ttttatggca ataaaaaaat agggtctgtg acatcaggga cattttctcc
     5581 aacttttcaa aaatctatag ggatggcaat gatagatatt gactttgcca gggctgggac
     5641 tgttttcgat ataatcataa gggataagat gaatacggca gaggttgtgc caaaaccgat
     5701 ttataaatat gtcagaaatt tttcatccag cctatgattg acggcttaaa gcaagagctt
     5761 cacatgaagt aaatttctga gcatgctcaa gaacacgccc gatctttagg tcggcggttc
     5821 ttgacatgtc agaaatcgct tcgggcgcac gctctgatga tttagcccag agtaagccag
     5881 cgatttctga gcatgctcaa gaacacgccc cgacctttag gtcggcggtt cttgacacag
     5941 tagaaaaatt aataaatgag ggcagaacca atcttttaca atggaaaatc caaaagattt
     6001 acgctacagc aaagagcatg aatgggtaaa gatcgagaat ggagtggcaa caatagggat
     6061 aactgatttt gcccaacacc aacttacaga tattgttttt atcgagctgc caactgcagg
     6121 aaaaaaagcc gagcagcaca agcaaatagc tgtcatagag tctgtcaagt ctgtttcaga
     6181 tgttttttct cctgtaagcg gagatatcgt tgaagtcaac agcaaacttt cagataatcc
     6241 tgagataatc aacaaagatc catacggcga agggtggata gcaaagataa atgcaaagga
     6301 cgccaaagag cttgacaacc tgctcacggc cgatgcatac agcaaactga ttcaataagc
     6361 tattattttt aagaggggtg gataggaaaa taggtgaaag aatggaatat atctgtcata
     6421 ctgaagaaga catcgataag atgcttaagg caataaagaa gaagaatatt tctgaacttt
     6481 ttaaggatgt tccaaaaaaa ttattatcat ctggactcag ggttccacag gggctttctg
     6541 agatagagct taagaggctg atgaatggac ttgcatcaaa aaataagact gagctaacct
     6601 attttcttgg agctggagca tacaaacatt ttatcccttc tgtggtcaac catatactat
     6661 ccaagcctga gttctatact gcttatacac cttatcagcc tgagatgtcg caagggtttt
     6721 tacaggcaat atatgaatat caaagcatga tatgcagcat aacagggatg gacgcctcca
     6781 acgcttctat gtatgacgga gcatcagctc ttgcagaaag ctgcataatg gcatgcaata
     6841 tcacaaaaag gaacaagata atagtctcta gggcagtgca ccctcattac agggaggttg
     6901 taaggacata ttgcaaagcg cattcaatag tccttgaaga agcagaatat gaaaatggtg
     6961 tgactagctc tgtaaatgtt gatagggata ctgctgcttt aatcatacaa agccctaact
     7021 tttttggagg catagaggac atcgagagct tttctaagat ggtgcatctg aatggagcgc
     7081 tccttaatgt ctgcgttgtc gaaccaacat cgcttggaat attaaagagc cctggaaaac
     7141 tgggggcaga catctttatc ggcgaagggc agagttttgg aaatcctgtc agctttggcg
     7201 ggccatatct ggggatactt gcaacaaaga aagagtatgt aaggcagctt cctggaagga
     7261 tagtgggaaa gactgtcgat accgaaggaa aagaaggatt tatccttacc ttgcaggcaa
     7321 gagagcagca tataagacga gaaaaagcgt cttcaaatat ctgctcgaac caggctttaa
     7381 atatgcttgc tgcggctgta tatttatcat acatggggaa atccggcttt agggaagcgg
     7441 caagccagag tgcacagaaa gcgcattatc ttgctaaaga actagagaag gtaggtgtca
     7501 atccctgctt taactcttca ttttataatg agtttgtcgt aaaggtcagc gatgttaagc
     7561 ctgtgcagga gaggctgctt tcgaaaggaa tcgtgtttgg gcttctcctt ggagactatt
     7621 atcctgagct aaacgactgt gttttggtct gtgtgactga gcttaacgct atcgaagaga
     7681 tggaccaagt tgttgaggcg ttaaaatgag gcttattttc gaactaagca aagaaggaag
     7741 aatgggggca agccttcctg agttggacgt acccttcaag gaggggctga tagaggatag
     7801 gtttgtatcg cttgacgccg agctgccgga gatctctgaa gtcgatgttg tcaggcatta
     7861 taccaatctt gcaaggcgaa acttcggggt tgacaacggg ttttatcctc ttggatcatg
     7921 caccatgaag tataatccca aagtaaatga ggatatctcg agattttctg gctttacaga
     7981 atgccatccg ctgcagcctt tttctcaagg cagccttgaa ttaatctata ggctcgaaag
     8041 attgctctgt gagatatgcg ggatggatgc ttttaccttg cagccggctg ccggggctca
     8101 tggagaactt acagggctta tgatgattaa ggcttacttt gaagacaagg gagaggctag
     8161 aaagaagata ataacaacag acagctcaca tgggacaaat cctgcgtctg cttcgatgtg
     8221 cggctttgag gtgatacaga taaaaagcaa tgaagacggg cttgttgatg ctgctctcct
     8281 tgacgatgca cttgataagg aagttgctgc tgtcatgctt accataccaa acactttggg
     8341 attatttgaa aaagatatcg ttaagatagc taaagccgtg catgaatatg gaagcctgct
     8401 ttatatggac ggtgctaaca tgaacgcgct tcttggcact gtacggccag gtgaaatggg
     8461 tgttgatgtc atgcacctga acctgcacaa gacctttagc actccgcacg gcggaggagg
     8521 gcctgggtct ggacctgttg gtgtgaaaaa agagcttgct gtgtttttgc caaatccaac
     8581 tgttgtttct aatgatggag cctatgagtt ctctaataac gttaaatcaa tcgggagagt
     8641 cagggctttt tatggaaatt ttggagtgat ggtaaaggca ttttcctata tccgggctct
     8701 tggaagagat ggccttagaa gggtgagtga gagcgctgta ctgaatgcaa actatatcaa
     8761 agaagcgctg aaaaaagact ttaagctgcc ttatgacagc ctatgcaagc acgagtttgt
     8821 aatatctgat gaagggctgc ctaatcatgt gacgactgct gatatcgcca aaaggcttat
     8881 cgattttggg atgcatccta tgacaatata ttttcctctt atagtacatg gtgcgatgat
     8941 gatagagccg acagagacag agagcaagga gacgcttgac aattttattt tggttatgaa
     9001 gcagataaga aaagagatcg atgaggaccc ctcaatcgtg ctttctgcac cccataatac
     9061 tccggtgaag aggctggatg cagtcaaggc tgcaagggaa ttggatttga ggtatcaata
     9121 tccaaagaaa gcgattgttg agagggttgt tccaagcaga tgaggccttc ttaggcgtcc
     9181 gaatggatta taattgttca atgaatttct gaaatatctt aggaagctgt aatctttgat
     9241 attatagtag agggatagga acgcatattt tacggtcaag ccaacagtct ttgctgcgag
     9301 atagaagaag gacgaagcag cgatcgaagc aatatagcca taacctaaag ctaaggagtt
     9361 taatagggtc cttatagatc tgctggcgct atatgctgcc accataaagt cccaaagcct
     9421 gggcttttct atcctttctt caagtgattc ttcgcccata tgattgcagc tactgtgaaa
     9481 gataaaactg ttagttgttg tcttgtacca caacacttat aagataaagg ctatttgaca
     9541 ctggaatgat agagcaaagc cttgaaaagc tggggtttag tccatcagag ataaaagtgt
     9601 acctgcagct tctcaaggga tgcagctatg caaataagct tagtgctgaa acagggataa
     9661 accgaacgaa cgtatacgaa gcgctcgata ggttaatagg aaaaggtgtt gtttctttga
     9721 tcactaaaaa taaagttaga tggtatgatg caaaagatgc aaattcactt aactcgatga
     9781 ttgatgagag gcaaggtgag cttgcaaaat tgaaagcaga tattgctgct gatatcaaag
     9841 atattgcagt gaatgaagaa aagctggagg caaccatctt tacaggcaaa aaagggctaa
     9901 ggatgctttt tgacgagatg atagaggttg gaaagcccat atccatcata gcatcaaacc
     9961 accaattcaa ggagttcttc gggccttatt tcgagctttg gcataagatg agaatagaga
    10021 gagggataag gcagagaagc atatttcctt taaagtttga gggaaagctg gagaaaagaa
    10081 agctgcttac ttacaaattt gtagatgata ggtattctaa tccgactaca actgtaatct
    10141 ttggcgataa ctgcttactt atacagtggt ctaatgaacc tgttgctgtt aaaattttca
    10201 ataaagagat taccaggtca catctgaatt attttaatat gatctacaag aaataaggtg
    10261 aaagaacaat aataattatt atcaggaaca aggcaaggct tacataaagg aatatcttgt
    10321 ctgcgagttc ttttttcagg tatctcttca gtgtcactaa aagcatcctt ccgccgtcaa
    10381 gagggccgat aggcgctaga ttgaagaggc ctataccgag gtttaggaag aacaagacat
    10441 tgatgaggcc gataagccag aggatgaagg aagccagggt tttgccgttc ttttgctcaa
    10501 agcttgggtt gattccaaac ttctcagaga tattcactcc catataggct tgggctttgc
    10561 tgcccggatt ttctgcgaga gtgaggttgt atactgtcct gtttgtttgc agctcgatga
    10621 tgtctcctgg ctttttttct gctaaaacct ttgaaagctc tgagcggctg tttatcttct
    10681 ggtcgtctat actgatgatg ctttcatttg ctgttatgcc tgcctgctct gcgccataac
    10741 cttcagcgac gcctgtaatc atcacttctt gagtgactat attgttcagg ataaaaggtg
    10801 tcagcaacaa cataattccc aggaaagcaa aagctgcaat aatgtttgca aaagggcctg
    10861 ctgcaaagac gctcaattga ggcccgatag gctttttgtt aagctgagct tcgtcaggct
    10921 ctacaaatgc tgcagggatt attggaacaa cagctcccag gaaagcaaag ccgcttgatt
    10981 taacctttac attatgagcc cttgcaagaa gaccatggga gaattcatgg atgactgcaa
    11041 ttataaatat agagattatc caatataaga aagggacata gaatataccc tttgcctgga
    11101 taggcagtac caaagctgct ccttggactg cgcttggttt tgtaaaaagg ctatacatgt
    11161 tatctacaag cgcgtaggtt atcaggacca taccgatgaa gccgaggaca acacctatgt
    11221 atccaagagt tttgataaag cgaggaaacc tttttgctgc cctgtccatc caggaaaggc
    11281 caatcttcgt cttatacatg gcgaagtata gcaacgggaa caacagtatc ttatggatag
    11341 taagcttttt tcggttaatg ataagaaata cgagcataaa tatgacaaaa accaaggcag
    11401 caatagtttg aatatcgacc atcaaggcat ttaagaaatt aggtttttta aatgttgtta
    11461 tttaagggct tgttggacaa aaattaaaaa atatcctatc cctaaagaaa tgatcaataa
    11521 aaaataaact atttatttcc ttctaaccgg cctaaatgta tgagcaccgc atcttttgca
    11581 ggttatcttt cctgcaagga cctttaggtt tggggctctt tgcttttgct tgcagttcct
    11641 gcaaacaaag acatttgcaa atttcctttt ctctgcttcc ttgaacttta ccattatccc
    11701 tgcacctgct tcatgacttt atcgttaagt attacccaat acaggacgtt acttccgctt
    11761 ctgacctgct cttttaattc atcgggtatt tgcatgtcaa aggtctcaaa agactcattg
    11821 tccattatgt ttgccatatc gcctgttatc gataagacct gtgcggtctt tttttcgatg
    11881 actggagcat caagcttatc atggcctggc atgacagcaa cccttttctt gctatctaca
    11941 atccctactg cggtaagcct tacctttgca tggccgtgct ttcctggcct tgaggtctga
    12001 gtatcagtaa caacgcaagc tgcgccttcc aaaacaacat aacttccttt ttttagtcca
    12061 ccaacatcaa ctggttttgt cgtcatttta tcacctattg cctgacagca agcctaatat
    12121 cctcttcttt tactgtcttt cttcctgcgt gtgttgccag gcgccatgcc ttttctgcta
    12181 tctcctctgc tttttcttcc atgacagttc tcagcgcttc ttttgctttg tctgaaaccc
    12241 taacatcgct gccaccaact ttcttaatta tcttttccat cgcagccagt ggaagtagct
    12301 tatctgacat gaaaatcacc ttttttgagc ttattttcct gtttaaagga tatttatcag
    12361 ttggtttgga atggtgtttc ctatataaag gttacttttt cccagacttc cccgatgtag
    12421 cagaattttt cagtccgcaa tctcagcatt tcgaaaaaat atcagattca taacaaaaat
    12481 tgacacctga gcatttgcgc tttttggtgc aagagcagca attctgtatt ctggcgtctg
    12541 a
//
LOCUS       DASRUK010000100        11996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_245411, whole genome shotgun sequence.
ACCESSION   DASRUK010000100 DASRUK010000000
VERSION     DASRUK010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 11996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11996
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245411"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="VFF28_04300"
     CDS             complement(<1..517)
                     /locus_tag="VFF28_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZX44878.1"
                     /translation="MIANYNPLEIEKEILLYWEKNKIYSKVKKKGKGKEFYFLDGPPY
                     TSGRIHLGQAWNKSLKDSILRYKRMKGFNVWDRAGYDMHGLPTEHKVMEKLGLKTKDD
                     IPTIGIEKFTTECRKLCVEFMNLMSKDFVRMGVWMDFDNAYQSIKNEFMEGEWWLVKK
                     AFENKRLYEGEK"
     gene            669..1526
                     /locus_tag="VFF28_04305"
     CDS             669..1526
                     /locus_tag="VFF28_04305"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZX44879.1"
                     /translation="MNPFLGILFGLLSMLGYGLSNAIAKVPSGKIGSVRTVFYRNIFV
                     SLFLFIILLFTDFTLSPLYIILALIISLIGYIPLLFFYKALSVGKVGIVSPVANSSVI
                     WTILLSIIFFKESLTATQVLAMLFIITGIFLVSVNFRDLRNSSLFTLSSGVPYAIITS
                     LLWGLTFFLFKIPVNILGPFLTSFIIELGIAIYSGIHLYTSQKSLAIKEKSMLPYLIS
                     IALFGTAGTLFFNLGISLYSVSIVSALTFSSPWIAALYGKIVYKEKLSIMQYMAIAFV
                     VLGLVVISL"
     gene            complement(1528..2004)
                     /locus_tag="VFF28_04310"
     CDS             complement(1528..2004)
                     /locus_tag="VFF28_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44880.1"
                     /translation="MQRISTARKLGMRKGDVISIIMLDKGLSGSDLKFLEDEFDFYKE
                     DIDKFVSQYKNLGIVFKLNAIEEDCAKDWKGFLILRSGEVDRFSNMFAGSNCDKVLLI
                     TNKRLLNIDLDNINEKIEEMNFRIEKQNESALSDIRDMAPASRKGIFGRLIDVLKK"
     gene            complement(2017..2169)
                     /locus_tag="VFF28_04315"
     CDS             complement(2017..2169)
                     /locus_tag="VFF28_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44881.1"
                     /translation="MDDLLNIFNDVQALEPKCPTCDTKIMLGVTTKFFPKYKAHVCLK
                     CGGKLL"
     gene            complement(2371..3354)
                     /locus_tag="VFF28_04320"
     CDS             complement(2371..3354)
                     /locus_tag="VFF28_04320"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZX44882.1"
                     /translation="MMAKELDLYDRKILFALDINSRTKAAAISREIRLPKETVNFRIK
                     RLLKNKYIHHFYTMINASKLGYGMHKIFLKFNNMTTEIEKQVIDFLLNEKTCANLRIT
                     EGAYDICFLTMNKNPSELKDFMCRFCKRFGEYLMQRSMHTLVSSIRLNQKFLYLGTSV
                     RKTINHGDLGNYSLNKIELEIIKIISTNARKKLVDIANETGQDPKVISYHIGKLEQNG
                     IIAGYFTALNARLLNRSLVQVDIALRNPDKYLAIIEFFDHTGMCAFASEMLGRYDLTI
                     ELYVENDLQLRKVMSKFKKSFGGYLIFYEVSRIYQEYVINWSPYDGYSSGK"
     gene            3481..3744
                     /locus_tag="VFF28_04325"
     CDS             3481..3744
                     /locus_tag="VFF28_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44883.1"
                     /translation="MMRQSIAGSISLASGLVSVLLIIIPFIAGWHFVGQRQDAIISIL
                     FGINGFIFCKVGAWGSRYMRPALYLVLSGLMLGVVNSIFILFI"
     gene            3756..5696
                     /locus_tag="VFF28_04330"
     CDS             3756..5696
                     /locus_tag="VFF28_04330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZX44884.1"
                     /translation="MKIFFASQSFYPHIGGVSTYLLNLAKGLLQRGDEVTEVHLRPPY
                     EASEDIIEGIKVFRIPKEPLNNNLLKGYSNFKERVYRECHGEGDLFSTKPLVSYGYDE
                     YNSINMSIGDQIEELLEKNPTEIVHIHDFQLLLIYRNIPRGIPIILTWHIPFADSISK
                     NLKQFLVKHMNEYDKVIFSCWDYASSAIKAGLSAQKVKIIPPIANTELFRPKEACTKI
                     KDLYHIPEKSKIILCVQRIDSKSGHIQLIRAMPKVVEKFPDARLVFIGGESMTSKISD
                     ERQKYVSEVYSLIEKFNLKDNVVFTGNVEYNLLPDYYNSADIVALTSKNEGFGLAVSE
                     GMACGKPIIGTKVPGIAAQVKDGLNGYLVDAGDSESTAERIIKLLGSEELRKNMGEES
                     LRIIKDKFEISKGIDSHCHLYRSLIKEKTDWCLEKLKLDDVSAIITDFDRTITSSTGE
                     VDEQLLKELDALNKPLILVTGRRIDYVKSLYEKYPVWDCIIGENGSYIYFPADDLSLI
                     FDSDKLQKAKEMLVENNISSNNGHSIISVSKEDELKVKETLMGLKDSLSFKDNIDEMM
                     VTPKGVGKGLTVKLALNHLGIDLKKAIIIGDGENDIDLFTLPGFKIAVANAVDSLKVL
                     ADEVTKKPSAQGVLEAIAKLRL"
     gene            5728..5877
                     /locus_tag="VFF28_04335"
     CDS             5728..5877
                     /locus_tag="VFF28_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44885.1"
                     /translation="MADEQQPVEQKPVEQAPEEQKPMEKKEEKPAEKPKEAEKKEEKP
                     AENAQ"
     gene            5903..7183
                     /locus_tag="VFF28_04340"
     CDS             5903..7183
                     /locus_tag="VFF28_04340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZX44886.1"
                     /translation="MIPALNNYIGIAGKKAVDRIRQKAEYLAGKRVVHISSTSAGGGV
                     AEILNTLVFLMCDLGIDTEWRILLGSHSFFKITKGIHNALQGKNWEMTPSRRRVYLEF
                     CKRNSMINHINDNDIVIIHDPQPIGMIEEYRKKTTWLWRCHIDISKPNIGTMKFLLPY
                     IRRFDGMIVSSPKFKIKYLRNPQFIIHPSIDPLSQKNLKISKSKARSILSKRGIDTDK
                     KIISQVSRFDPWKDHFGVIRMYEKIKEKEDCQLVLMGDMAGDDPQGSTIYYKVKQKCD
                     ELKDVHLITEKNDLLVNALQQESAFVFQNSIKEGFALTVSEALWKKTPVIGTPVGGIP
                     LQIINNKTGFLIRSQAEGVKRGLQLLQDCRLRSRLGSQGHEHVKNNFLITRHLEDYID
                     LFNRFYPQPKFWKGVSLLKGIKLINPVSSRHRHA"
     gene            7176..8126
                     /locus_tag="VFF28_04345"
     CDS             7176..8126
                     /locus_tag="VFF28_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HZX44887.1"
                     /translation="MLDYLLLLIGLALLIKGADYLIDGSSSLAKKLGISSLVIGLTIV
                     ALGTSLPELIVNVYASIEGNSELILGNIIGSNLFNLLLILGITAVIVPLSVQSSTTWK
                     EIPFALLASVVLLVQANKSLFGAGKDVLTRADGIILLFLLAIFVYYVFQLMKTSNEVV
                     TDIHKHSNMAIALMLFGGLAALFIGGRMTVGSAVNIARQLGISEFLISATVIAAGTSL
                     PELITSIVAAMRKEMGISIGNIVGSNILNIFLILGISSLISPIRVPAGIAFDLIFLVF
                     SSLLLLAFILMGKKHQLKRWQGAVFILLFISYMSAIMIRG"
     gene            complement(8123..10564)
                     /locus_tag="VFF28_04350"
     CDS             complement(8123..10564)
                     /locus_tag="VFF28_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase"
                     /protein_id="HZX44888.1"
                     /translation="MAKITFFPIDITYKIESGKAVIYMFGRTADGKQVCVADRNFEPY
                     FYVIPKKGENVEEKLLKLKVESDGQTAEVVRTERIKKKFLGSEVNAVKVFTKLPSNVP
                     IIRQTIKDWEMLERVTEYDIKFTKRYLIDKNITPFVAVLCHGDFVSAKAKVPVFEISK
                     LEMKEESYEKPRLLALDIETYNPEGKNIDFDAFPIIMLSFYGNDYKKLITWKRFNTKL
                     DYVEFVKSEAELIERFREIVNEYKPDILTGYFSDEFDLPYIRTRADKYKLKLDIGLDY
                     SELQVKKTRVTTSNIVGINHLDTFKFFKKIMGSSMDTYSLSLNSVASALLNEKKAVVE
                     LDKLAYTWDNNPEDLSGYCEYCLQDSCLAFKIAEKLMPNIIEMVKIVGLTVPDISRMG
                     FSQLVESFIMKQASSFNELAPELPHHGQLGERQMHTYKGGFVYEPKPGLYHNIIVFDF
                     RSLYPTIISSHNIDPGTLDCECCEGKKVAPMEGRKKHWFCTKKKGFLPTLIEDLITRR
                     MRLKEIMKKKSTPLLEARSQSLKLLANAFYGYLGFPMARWYSIDSARATAAYGRFYIK
                     KVIDGAEGEGFKVVYSDTDSVFLTFDNKKKEDAFKFQEKINMDLPGIMELDYEGFYPS
                     GIFVSAKVGKFGAKKKYALVSEQGNLKIRGFETVRRNWSPIAKNVQEKVLIMILKEDK
                     PAEAFDYVKSVIKDLRDNKIPLKDVIIHTQLSKEIESYDNVGPHVAIATQMKNKGINV
                     SAGSQINFVITKHGERIRDKAKLPDEVTQDDYDPDYYINNQVVPAVEKIFEVLGYDKK
                     ELVESHEQNKLSRFF"
     gene            complement(10597..11139)
                     /locus_tag="VFF28_04355"
     CDS             complement(10597..11139)
                     /locus_tag="VFF28_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZX44889.1"
                     /translation="MTLELSEIKEIRKKYGLTQTQLAERAQVSQSLIAKIEAGRLDPT
                     YSNAKKIFEAIDSLGKKKEMKAEEMMNIHIISVKPDEGIRSAIEKMKKANISQMPVLD
                     EHKSIGIVSESIILEALLNRKGDKVKDIMEDSAPVVSKNTTISAVSNLLRFYPMVLVS
                     DSGELKGVITKSDLLGKIYR"
     gene            11246..>11996
                     /locus_tag="VFF28_04360"
     CDS             11246..>11996
                     /locus_tag="VFF28_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZX44890.1"
                     /translation="MVKDLVVILDEEGQNTARSLGYPYFGKSRGGKSSTYKIETPLGR
                     QRTVKGVLVKDAPLDEHTAEFLYLEDTDRCNFSCLGCGVGNNLTHVGLTHLLEDKSRY
                     LTPNFVTQLAGRIGNSPYANTTTRRIFHGGGEPLINPTALARVLRGLATVDNTTQLVL
                     TNGCNLPLDKEEFTAFMARLGNPYIVLTYTNSHEKFYEQLAKTGLYRQLIPSKVQAHD
                     SLKEKIKIIGGYCKHLNVDFAGHVVADAFPSP"
BASE COUNT         3375 a         2632 c         2377 g         3612 t
ORIGIN      
        1 ctttttctcc ttcataaagc cttttgttct caaaagcctt ctttacaagc caccactcgc
       61 cttccatgaa ctcattcttt attgactggt aagcgttatc aaagtccatc cagactccca
      121 tccgaacaaa atccttgctc atcaggttca tgaactccac acagagcttt ctgcactcag
      181 ttgtaaactt ctcgatgcct atggttggaa tatcatcttt tgtctttaag cccagctttt
      241 ccataacctt gtgttcagta ggaaggccgt gcatgtcata gcctgccctg tcccacacat
      301 taaatccttt catcctctta tatctaagta tagagtcttt aagcgacttg ttccaagcct
      361 gtccaaggtg aatccttccg gaggtatacg gcgggccgtc taagaagtaa aactctttgc
      421 ctttcccttt cttcttgacc tttgagtata tcttgttctt ctcccaatag agaaggatct
      481 ctttttctat ctccaaagga ttgtaattgg caatcatgga gtcaaagaga aatagggagg
      541 tttataaaga ttatgcgtta agctggtcca attcttcagc cgtatgctgc aatgttaatc
      601 aattaatcca aacatcttta taatcaatat cctttaatac attaacacaa ccagaacgaa
      661 ttaacccaat gaaccccttc ctcggcatcc tctttggtct cctttctatg ctgggctatg
      721 gcctcagcaa cgcaatagca aaagttccct caggaaagat tggcagtgtc aggacagttt
      781 tctaccgcaa tatatttgtc agcttattct tgtttatcat cctcctgttc actgacttta
      841 ctttatctcc tttatacatt attctcgccc ttataatctc ccttatcggt tatatccctc
      901 tgcttttctt ctacaaagcc ctgagcgtcg gcaaggtcgg aatagtctca cctgttgcaa
      961 actcatcagt tatttggaca atccttctct ctattatttt cttcaaggaa tctctgacag
     1021 ctacccaggt attggccatg ctctttatca taaccggtat cttccttgtt tcggtcaact
     1081 tcagagactt aagaaactca agcctcttca ccttatcaag cggtgtacct tatgcaataa
     1141 taacttccct cctttggggc ctcacctttt tcttgtttaa gataccggta aatatcctcg
     1201 ggccatttct cacatcattc atcatagaac taggcatagc tatctatagc ggcatccatc
     1261 tctatacatc acaaaaatcc ctggccataa aagaaaaaag catgcttccc tatcttatat
     1321 caatcgcact ttttggcaca gcaggcactc ttttctttaa cctgggaata agtctttaca
     1381 gtgtcagcat cgtctctgct ttaacattca gtagcccatg gattgcagct ttgtatggaa
     1441 agatagttta caaggaaaag ctttccatca tgcagtatat ggcgatagcc tttgttgtcc
     1501 ttgggctggt agttatctct ctttaaacta cttttttaga acatctatca atcgcccaaa
     1561 tatccctttg cgtgaagctg gagccatatc ccttatatcg ctcagcgcac tttcgttttg
     1621 tttttctatc ctgaaattca tctcttctat cttctcatta atgttgtcca aatcgatgtt
     1681 aagcagcctt ttgttcgtta taagaagcac tttgtcgcaa ttgctgcctg caaacatatt
     1741 gctaaacctg tccacttcac cgcttctaag gataagaaat cccttccaat cctttgcgca
     1801 atcttcttcg atggcattga gtttgaaaac tattccgagg ttcttgtatt gggagacaaa
     1861 cttgtcgatg tcttctttgt agaaatcaaa ctcatcttca agaaatttaa ggtcgcttcc
     1921 actaagcccc ttgtcaagca ttattatact gataacatct ccctttctca tccctaactt
     1981 ccttgctgtc gatatccttt gcattttatc aactccttat aataatttgc ctccgcattt
     2041 caggcataca tgcgccttat atttgggaaa aaattttgtg gtcactccaa gcattatttt
     2101 agtatcgcat gtagggcact ttggctcaag agcctgcaca tcgttaaaaa tattcagcag
     2161 gtcatccaca agcactaact cagagaaagc ttgtataaaa agttttcgtg agaggagtga
     2221 ctattgttgc ataataaaaa attatctttt gacatgtcag aaatcgcttc gggcgcacgc
     2281 tctgctgttt taacccagtg caagccagcg atttctgagc atgctcaaga acacgccccg
     2341 aggcttttca gcccggcggt tcttgacaaa ttatttgccg gaactatacc catcataagg
     2401 agaccagttg atcacatatt cctggtatat cctcgacacc tcataaaaga taaggtagcc
     2461 tccgaatgat tttttgaact ttgacattac ctttctcagc tgcaggtcat tctcaacata
     2521 caattctata gttaagtcat atctcccaag catctctgaa gcaaatgcgc acatacctgt
     2581 atgatcaaaa aactcaatta ttgccaggta cttatcaggg tttcttaggg cgatgtcaac
     2641 ctggacgagc gatctgttga gaagccttgc attgagcgcg gtgaaatatc ccgcgattat
     2701 cccattctgt tcaagcttgc cgatatggta gctgataacc tttggatcct ggcctgtctc
     2761 gtttgcgatg tctaccagtt tctttcttgc attggttgat attatcttta ttatctccag
     2821 ctctatcttg ttaagggaat aattcccgag atctccatgg ttgatcgtct tcctgacact
     2881 ggttcccaga tagagaaatt tttggttaag tctgatgctg cttaccagtg tatgcatgct
     2941 cctttgcatc aggtattccc cgaatctttt acaaaacctg cacataaaat ccttcagctc
     3001 gcttggattc ttattcatgg ttaaaaagca tatatcatat gctccttctg tgattcttag
     3061 gtttgcgcag gttttttcat taaggagaaa atcgatgacc tgtttttcga tctctgttgt
     3121 catgttgttg aatttcagga atattttatg catgccatag cccagcttgc ttgcattgat
     3181 catggtataa aaatggtgga tatacttgtt tttcaagagc cgcttgatcc tgaaatttac
     3241 agtttccttc ggaagcctta tctccctgga tatggccgcc gcctttgtcc ttgaatttat
     3301 atccagggca aagaggatct ttcggtcgta taagtcaagc tcttttgcca tcatccatag
     3361 aaataaatac tagtttttta taaattttac taaaaaaaag gaataattcc catctttttt
     3421 tccttttatt gcaataatta aatgccaatt tttaattacc cattgcaggc gccattatct
     3481 atgatgaggc aaagcatagc aggcagcata tcgcttgcat ccggactggt aagcgttctt
     3541 ctcatcatca ttcctttcat cgccggttgg cattttgtcg ggcaaaggca ggatgccata
     3601 atctctatcc tttttggcat taatggtttt attttctgta aggttggagc ctggggctca
     3661 aggtatatgc gccctgcttt atatttggta ttatccgggc tcatgcttgg tgttgttaat
     3721 tccattttta tcctattcat ataagaggtg aagtaatgaa aatatttttt gcaagccagt
     3781 cattttatcc tcatatcggg ggtgtttcaa cttatttgtt aaacctggca aaaggcctcc
     3841 ttcagagagg ggacgaggtg acagaagttc acttaaggcc gccttatgag gcaagtgaag
     3901 atatcatcga gggcataaag gtattcagga taccaaaaga gcccttgaat aataaccttc
     3961 tgaaaggcta cagcaatttt aaggaaaggg tctataggga atgccatgga gaaggagacc
     4021 tttttagcac aaaaccgctc gtttcctatg gatatgatga gtataacagc atcaacatgt
     4081 cgatcggaga ccagatagag gaattattgg aaaaaaatcc taccgagata gtgcacatac
     4141 atgatttcca gcttctgctc atctaccgca atattccaag ggggatacct ataatcttga
     4201 catggcatat tccttttgct gattccatat caaaaaacct gaagcaattt ctggtaaaac
     4261 atatgaatga atatgacaag gttatatttt cctgctggga ctatgcaagc tcagccatca
     4321 aagccggcct gtctgctcaa aaagtaaaga taatacctcc tatcgcaaac acggaattat
     4381 tcaggccaaa agaagcatgc accaagataa aagatcttta tcatatccct gaaaagagca
     4441 aaattatctt atgcgtccag cggatagatt caaagagcgg ccacatacaa ctgataaggg
     4501 caatgcctaa ggttgttgaa aagttccctg atgcaaggct tgtttttatt ggcggagagt
     4561 ccatgacaag caagatatca gacgagcggc aaaaatacgt ttcagaagta tacagcctga
     4621 ttgagaagtt caatcttaag gacaatgttg tctttacagg aaatgttgaa tacaaccttc
     4681 tcccagatta ttataactca gcagacatag ttgcccttac ttcaaaaaat gaaggctttg
     4741 gccttgcagt atcagaaggc atggcctgtg gaaagccgat aattggcaca aaggttccgg
     4801 ggattgctgc tcaggtgaaa gatggcttga acggatatct tgtggatgca ggggactctg
     4861 agagcactgc tgagaggata atcaagctcc tcggaagtga ggagttaaga aaaaacatgg
     4921 gagaagaaag cctgcgcatt atcaaagata agtttgagat ctcaaagggc atcgacagcc
     4981 actgccatct ttacaggtcg ctcatcaagg aaaaaacaga ctggtgcctt gagaagctca
     5041 agcttgacga tgtctcggcg atcattacag attttgaccg aacgatcaca agcagcacgg
     5101 gcgaggtgga tgagcagctg ctaaaagagc ttgatgccct caataagccg cttatccttg
     5161 ttacaggcag gcggatagat tatgtcaaga gcctttatga gaaatatccg gtgtgggatt
     5221 gcataatcgg agagaatgga agctatatct attttcctgc ggatgacctt tcgttgatat
     5281 ttgattctga taagctccag aaagcaaagg agatgcttgt tgaaaacaat atctcttcaa
     5341 acaatggcca ctctatcatc agcgtgtcaa aggaggatga gctaaaagta aaggagacat
     5401 tgatggggct aaaggacagc ctcagcttta aggacaatat cgatgagatg atggtcacgc
     5461 caaaaggcgt tggaaaaggc ttaacagtca agcttgcatt gaaccatctg gggattgact
     5521 tgaagaaagc gattatcata ggggacgggg agaatgatat agaccttttt acccttccag
     5581 gatttaagat tgcagtggca aacgcagtgg acagccttaa ggtcctggca gacgaagtta
     5641 caaaaaagcc ctcggctcaa ggggtgcttg aggcaatagc gaaattaagg ctatagctca
     5701 ctatcataca tttaggaggt gtataaaatg gcagatgaac aacagccagt agaacaaaaa
     5761 ccagtagagc aggcgccaga ggaacaaaag cctatggaaa aaaaggaaga aaaaccagct
     5821 gaaaagccta aggaggctga aaaaaaggaa gaaaaaccag ctgaaaacgc acaatagaag
     5881 agaatttttc tcttctttta ttatgatacc tgcactaaac aactatattg ggattgccgg
     5941 gaaaaaggct gtagatagga taaggcaaaa agccgagtac ctggcaggaa agcgcgtagt
     6001 tcatataagc tccacatcag ctggaggcgg ggttgcagaa atcttaaaca cccttgtctt
     6061 tctgatgtgt gacttgggca tagacactga gtggcggatt cttctgggaa gccattcatt
     6121 ctttaagata accaaaggga tacacaatgc actgcagggc aagaattggg agatgacacc
     6181 aagtcgcagg agggtttatc ttgagttttg caaaagaaat tcgatgatca atcacataaa
     6241 cgacaatgac atagtcatca tccatgatcc gcagcctatc ggaatgatag aggaatatcg
     6301 gaagaagaca acatggctgt ggaggtgtca tatagatata tcaaagccaa acatagggac
     6361 tatgaagttt ctcctgcctt acataagacg gtttgatgga atgattgttt cttctcctaa
     6421 atttaagata aaatacctaa gaaaccctca gtttataata cacccaagca tcgatccgct
     6481 ttcccaaaag aacctcaaaa tcagcaagag caaggcaaga agcattctct caaaaagagg
     6541 tatagacaca gataaaaaaa taatctcgca ggtctcaagg tttgatccat ggaaagacca
     6601 ttttggcgtt atcaggatgt atgaaaagat aaaggaaaaa gaggactgcc agctggttct
     6661 tatgggagac atggcaggcg atgatcctca gggttcaaca atatattata aagtgaagca
     6721 gaagtgtgat gagctcaagg atgtccatct gataacagag aaaaacgacc tgcttgtcaa
     6781 tgcacttcag caggagtcgg cttttgtgtt ccaaaattca atcaaggaag gttttgcttt
     6841 gacggtttca gaggcgttgt ggaaaaaaac accggtcata ggcactcctg tcggaggcat
     6901 ccctctgcag ataattaaca acaagaccgg ctttttgata agaagccagg cagagggggt
     6961 aaaaagaggg cttcaattgc tgcaggattg caggctaagg tcaaggcttg gaagccaggg
     7021 ccatgaacat gtgaagaata attttcttat aacgaggcat ctggaggatt atatcgacct
     7081 tttcaacaga ttctatcctc agcctaagtt ttggaaaggc gtctctttat taaagggcat
     7141 aaagttaata aaccctgtca gcagcagaca tcgccatgct tgactatctg ctgctcctca
     7201 tcggccttgc ccttctgata aagggtgcgg attatcttat tgacggctcc tcctctctgg
     7261 caaaaaagct aggcatctcc tcacttgtta tcggcctgac gatagttgcg cttgggacat
     7321 ctcttccaga gcttatagta aacgtctatg caagcataga aggcaattcc gaactcatac
     7381 ttggaaatat catcggcagc aacctgttta acctccttct tatcctgggt ataaccgcag
     7441 tcatagttcc actaagcgtc caatcttcaa caacttggaa ggagatacct tttgcgttgc
     7501 tggcatcagt tgtattattg gtccaagcaa acaaatcact ctttggggca ggaaaggatg
     7561 tccttacaag ggcagacggc attattctct tgttccttct tgccatcttt gtatattatg
     7621 tattccagct gatgaaaacc tccaacgagg tggtcacgga tatccataag cattccaata
     7681 tggcaatcgc tctcatgctt tttggaggcc ttgccgcact ttttatcggc ggcaggatga
     7741 ctgtcggctc agcagtaaac atcgcaaggc agctcggcat aagcgagttt ttgatatcgg
     7801 caacagtcat tgcagcaggc acttcccttc ccgagttgat aacatccata gttgcagcga
     7861 tgagaaaaga gatgggcatt tcaattggaa atattgtcgg ctcaaacata ctgaacatat
     7921 tcctcatttt agggataagc tctttgatct cgcctataag ggtgcctgca gggatagcct
     7981 ttgaccttat tttccttgtc ttttcaagcc ttcttctcct ggctttcata cttatgggaa
     8041 aaaagcatca gctcaaaaga tggcaaggag cagtgtttat tctattgttc atatcttata
     8101 tgtcagcaat aatgattagg ggttaaaaaa acctgctcag cttgttctgt tcatggctct
     8161 ccacaagctc tttcttatca tatccaagaa cttcaaatat cttctcgaca gcagggacca
     8221 cttgattatt gatataatag tcagggtcat agtcatcctg cgtaacttca tcaggaagtt
     8281 tggccttgtc tcttatcctt tcgccatgct tggttatcac aaaatttatc tgagagcctg
     8341 ctgagacatt tatccctttg ttcttcatct gcgtagcaat agcgacatga ggccctacat
     8401 tatcataact ttcaatttct ttagaaagct gggtatgtat tatcacatct ttcaaaggta
     8461 ttttattatc cctaaggtct ttgatcacag attttacata atcaaacgct tcagcaggtt
     8521 tatcctcctt aagtatcatg ataagcactt tctcctgaac attctttgct atagggctcc
     8581 agttgcgcct tacagtctca aatcccctga tcttcaggtt tccctgttca gagaccagtg
     8641 catatttttt cttagcccca aacttgccaa cctttgctga cacaaagatc cctgaaggat
     8701 aaaagccctc ataatcaagc tccattatcc cgggcaggtc catatttatc ttttcctgga
     8761 acttgaaggc atcctctttc tttttgttgt caaaagttaa aaaaacacta tcagtatcgc
     8821 tatacactac cttaaatccc tctccctctg cgccatcgat gacctttttg atataaaacc
     8881 tgccatatgc agcagtcgct cttgcagaat ctatgctgta ccatcttgcc attggaaaac
     8941 caagatagcc ataaaatgca ttagccagta attttaagga ttggcttctt gcttccagca
     9001 ggggagtcga ctttttcttc attatctctt taagcctcat cctgcgggtg atcaagtctt
     9061 caataagcgt cggtaaaaat ccttttttct tcgtgcagaa ccaatgcttc ttcctgcctt
     9121 ccataggcgc aaccttcttt ccctcacagc actcacagtc aagcgttcca gggtcgatat
     9181 tatgggagct gattattgtt ggatataggc ttctgaaatc aaatacgatt atattatggt
     9241 acaggcctgg ctttggttca tacacaaagc ctcctttgta agtgtgcatc tgcctctctc
     9301 caagctgccc atggtgggga agctctggtg caagctcgtt gaaggaagaa gcctgtttca
     9361 tgataaaaga ttccacaagc tgggaaaatc ccatccttga gatgtcaggg acagtaaggc
     9421 cgacgatttt caccatctcg atgatgttag gcataagctt ttctgcgatc ttgaaggcaa
     9481 ggcatgaatc ttgaaggcag tattcacagt agcctgaaag atcctcagga ttgttatccc
     9541 aggtataagc aagcttgtcc agctcaacta cagccttctt ctcatttagt agggcgctcg
     9601 ccacagaatt aaggcttagt gaatatgtat ccatcgaaga gcccattatc ttcttaaaaa
     9661 atttaaaggt gtcaaggtgg tttatcccga caatattgct cgttgtcact ctggtctttt
     9721 tgacctgcag ttcagaatag tcaagcccga tatccagctt cagcttatat ttgtcagctc
     9781 ttgtcctgat ataaggcagg tcaaactcat ctgaaaaata gcctgttaag atatccggct
     9841 tgtactcatt gactatctcc ctaaaccttt cgatcagctc tgcttcgctt tttacaaact
     9901 ctacatagtc cagctttgtg ttaaatctct tccaagtgat gagtttcttg taatcattgc
     9961 cgtaaaaaga aagcatgatt atcggaaagg catcaaagtc tatattcttt ccttcagggt
    10021 tatatgtctc gatatcaagc gcaagaagcc ttggcttttc gtaggactct tccttcatct
    10081 caagtttgct tatctcaaaa acagggactt ttgctttcgc gctgacaaaa tcgccatggc
    10141 acaagacagc gacaaatgga gttatgtttt tatctattag gtatcttttg gtaaatttga
    10201 tatcatactc agttaccctt tcaagcatct cccaatcttt gatagtctgc ctgattatcg
    10261 gcacattgga cggaagcttt gtaaagactt tgacagcatt gacctcactg ccaagaaact
    10321 tcttctttat ccgttcagtc cttacgacct ctgcagtctg tccgtcgctc tcaactttga
    10381 gcttcagcag tttctcttca acattctctc ccttttttgg gattacatag aaataaggct
    10441 caaaattcct gtcggcaaca cagacctgtt ttccatcagc agtccttcca aacatataga
    10501 tgacagcttt gccgctctct atcttgtagg ttatatcaat cggaaagaaa gtaatcttgg
    10561 ccatgtgtat aagtatctat ggtagattta taaaatttac ctataaatct tccctaagag
    10621 atcagacttt gtgattacac ccttaagctc accgctgtca ctgacaagga ccataggata
    10681 aaacctaaga agattagaca cagcgcttat cgtagtgttt ttggaaacaa caggagcaga
    10741 atcttccatt atgtctttga ccttatctcc cttcctgttt agcaaagcct caaggattat
    10801 tgattctgac actatcccta tagacttatg ctcgtccagg acaggcatct gagagatatt
    10861 tgctttcttc atcttttcaa tagctgatct gatcccttca tcaggcttta ccgagattat
    10921 gtgtatattc atcatctcct ctgctttcat ctcttttttc tttccaagcg agtcgatagc
    10981 ctcaaatatc tttttggcgt ttgaatatgt cggatcaagc ctgccggcct ctatctttgc
    11041 gataaggctt tgggatacct gggccctttc tgcaagctgg gtttgtgtca ggccgtattt
    11101 cttccttatt tcttttatct cgctgagctc caatgtcata atcaacgaat aaatcaaggt
    11161 tatatttatt ctttatggaa ttgaaattct tattggaata aattagacag aacattattt
    11221 aagcaatacc ttttctgaaa agccaatggt aaaagatttg gtagtcatct tagatgagga
    11281 aggacaaaat accgcacgaa gcctaggtta cccctatttt ggaaaatcaa gagggggcaa
    11341 atcttctacg tataaaattg aaacaccgct tggaaggcaa agaacggtca aaggagtact
    11401 ggtaaaggat gctcctttgg atgagcatac tgcagaattc ctatatctcg aagatacaga
    11461 ccgatgcaat ttttcttgtt tgggttgtgg agttggcaat aatctaaccc atgttggcct
    11521 tacccatcta cttgaagata aatcaaggta tcttaccccc aattttgtta cacagctcgc
    11581 tggtagaata ggcaattctc cttatgccaa tacaacaaca agacgaatct ttcacggagg
    11641 aggagaaccg ttgatcaatc ctacggcctt ggccagagtg ctcaggggtc ttgccactgt
    11701 agacaataca acacagcttg tgcttacaaa tggttgtaat ctgccccttg ataaagagga
    11761 gtttactgcc tttatggcac gtttaggtaa tccatatatc gttcttactt atactaattc
    11821 ccatgagaag ttttatgaac aactagccaa aaccgggctg tatcggcaac taattccttc
    11881 taaagttcaa gcacatgatt ccttgaagga aaaaattaag attatcggtg gctattgcaa
    11941 gcaccttaat gtcgattttg ccgggcacgt agtggcggat gcatttcctt ccccct
//
LOCUS       DASRUK010000101         7272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1598313, whole genome shotgun sequence.
ACCESSION   DASRUK010000101 DASRUK010000000
VERSION     DASRUK010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 7272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7272
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598313"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(486..749)
                     /locus_tag="VFF28_04365"
     CDS             complement(486..749)
                     /locus_tag="VFF28_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44891.1"
                     /translation="MFKGKNVRVVLVGDALDEYKQLNKIVGDEIKGGINSSENQTLLN
                     SLKRIFDLLKENPQYGFYIQKHLIPKEYIINATSPVTGGRFTL"
     gene            complement(752..1048)
                     /locus_tag="VFF28_04370"
     CDS             complement(752..1048)
                     /locus_tag="VFF28_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44892.1"
                     /translation="MKRKEWKVKDTGKRKEVIHYPNLKTVLMVEEILKKADTPLSKNE
                     ILRRMPKKTIRQTLRVIIDYLEYSGKIVDGDKGVVWTFNENKKLDAAIKAGVEV"
     gene            1319..1786
                     /locus_tag="VFF28_04375"
     CDS             1319..1786
                     /locus_tag="VFF28_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44893.1"
                     /translation="MEELYAKITGLFGDIVRGQGLNDSLLAMILARLYIEPEPIAMDE
                     LAKETGYSLASVCNKIKMLGPLVTVSKKPGSKKIYLFMDKNIIGIWKDMLAKKQQVLA
                     STKEKLPLIIKESRKRAKTADDKKRVEIVEGFFDQISKVEGLLKKMMDGFDEL"
     gene            1783..2469
                     /locus_tag="VFF28_04380"
     CDS             1783..2469
                     /locus_tag="VFF28_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZX44894.1"
                     /translation="MNNLVIELEDVWKIYKMGEVEVPALRGLNLKVKRGEFLAIMGAS
                     GSGKSTAMNMIGCLDVPTKGRVLLEGEDISKLSESDLAQIRGKKIGFIFQQFNLIPTL
                     SAAENVTLPTIFQNVDKEKRMKKAKELLEMVGLGDRINHKPNELSGGQQQRVAIARSL
                     VNDPELILADEPTGNLDTASGHKVIDFLKEMHSKEKKTIVMVTHDREVARNAERTEVL
                     SGGRIIRRAK"
     gene            2466..3710
                     /locus_tag="VFF28_04385"
     CDS             2466..3710
                     /locus_tag="VFF28_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44895.1"
                     /translation="MKKIIVVLLFFVLALSSVVMGASSGLNSYWINVTMINQEPDPAE
                     AGRYVTLRFKLENTGTDIAKDVTVELLIKYPFSLEPGESATKYIGSIRARQMGDTGVI
                     VDYRVRVDEKAIEGENEVELRYKLSNQDWVKPEAFTVDIGVHDILLNIESIDSPRMIR
                     PGAISNIDVKLKNLAQSFVKEIKVTLELTGIPIATIGETNRKIISQMEGLSESTVQFN
                     LMAEPDAESKLYKVPIKLEYRDRIGNLYMKNETVGLIVGDEPDIAASIDTSTVYSSGR
                     TGDVSVKLVNKGVTDIKFMTVKIGDGQYKVLSAKEVYVGNIDSDDYESADFKLSLSRV
                     KGNEVALPLRLEYEDANNNEYTQDVTVEMPLYTASEAKALGLSKGSSGVGLFIVLLIV
                     GAGIYAYIRIKNKKPLIPFRRK"
     gene            3712..4926
                     /locus_tag="VFF28_04390"
     CDS             3712..4926
                     /locus_tag="VFF28_04390"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZX44896.1"
                     /translation="MLRDYFVFSFHNLKSRRLRSFLTVIGIFIGVLAVVSLVSLGEGL
                     RQAIISQFGISSTEVISVQAGGLSGYGPPGTAVVNPLTEDDALAIERINSVERAVSRS
                     IENIKVEFNDRVDFTYATDIPDGDDRKFVYAQVELEAIEGRLLREGDNRQVALGYNFA
                     EDDKFGKRVFVGDKILVNNEEYGVVGITAKKGSFIFDNVVYFNRDALYELMEIEDDVD
                     IIAVQVKDKSLIQEAEEDIEKLLRKRRDVKEGNEDFEVSTPEASLSSVNQILLAVQSF
                     IAMIALISVAVGGIGIVNTMTTSVMERRREIGIMKAIGARNRDIFMQFFIESGLLGLV
                     GGLLGVIVGVIVGLIGTAGINSFVGAESTPQINLILIILTLLGSFLIGSLAGIIPALH
                     AAKQKPVDALRS"
     gene            4965..6128
                     /locus_tag="VFF28_04395"
     CDS             4965..6128
                     /locus_tag="VFF28_04395"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZX44897.1"
                     /translation="MERKARSFLTILSIFIGITTIFIFVSFGWGLFDYVESVSSSSAA
                     ELALVQARGGGAPGLADVFALTEDDLEAVQSVKGVYEATGAYFKAAEVKQGENRKYVF
                     LIGYDPDIPLIMNSFNIDIDKGRKLDDGDDGKVLLGYNYQVPNKIFPKGYKTGDSIEV
                     QGQKLKIVGFFKPVGNPQDDSQMYVINGYIDELYDEELNYGMIVARLDKTDMKGTIDR
                     IKKEVRQERDLEEGKEDFFVSSYEDLMKTYLVVLNVIVGFIVLIGLISVLVSAVNTAN
                     TMITSVLERVREIGVIKSIGARNKEIFKIFLFESSFLGFVSGVIGVFFGWVITFAAGQ
                     LLDAAGWGFLSPHYSFLLFFGCIMFATLTGAVSGVIPAYKASRLKPVDALRYE"
     gene            complement(6200..7111)
                     /locus_tag="VFF28_04400"
     CDS             complement(6200..7111)
                     /locus_tag="VFF28_04400"
                     /inference="COORDINATES: protein motif:HMM:NF004911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZX44898.1"
                     /translation="MQSVHFERAIFISWYCSKRDCAFCYLSSKTNPQSPIKDRRSLSS
                     ILAEAVICKACGWKVEFISGGCDTYTDKELLEIIKGIYEITGQKQWLNLGILTEKQLN
                     LFKPYIEGVCGTVECITPGLRDKLCPSKPLKDIEQMFRIAEKLGLKKTITLIVGLGET
                     IEDFKNLKSFIEKNTVGKLTFYRLKPQKGTVFENTKGPDTDYYVRWIKNTRQAFPNIH
                     IVVGSWLTHLDEIHLLLKAGANSITKFPSIKQFNSKYARQIEQEVHKAGMKFEGTLTK
                     VPKITLPHGQLKDKLKAKIDEYISQMD"
BASE COUNT         2114 a         1356 c         1712 g         2090 t
ORIGIN      
        1 tggcaccaaa atggtagcga tgaggagata ccttatgttt gataggtaca gggatgtctg
       61 ttccatcaaa gctcatgtca tcgttttcaa acaccttctg actttctaat acatcgtcga
      121 caaatacaaa tatttgcatg acttaaatgg aattatactg aaatactttc ttaagactat
      181 tatctgaaaa acaataggga ggattactat ctccctcttg ccatctttgc ttctgccaca
      241 tggccctgct tgtcaatgta gtaaagatag ccttcttttt tgtcagcgcc gatcttagct
      301 ataacttctt gctccttatc tttcttctag ccggcccttg ccatcttaat tctggcaacg
      361 tctcctttct tgttcaggaa atacagccat cctcgctcca ttctatttat atccaaactt
      421 cttctcataa gccttgtgat caagaatgtc aagaataaca tctacaatct ccagagcatc
      481 gctctttata gtgtaaatcg cccgccagtt accggcgatg ttgcatttat gatatactcc
      541 tttggaatta ggtgcttctg tatataaaaa ccgtactggg gattctcctt aagaaggtca
      601 aatatcctct tgagcgaatt tagcaaggtt tggttttctg agctgttaat ccctcctttt
      661 atctcatctc cgacaatctt gttcaactgc ttatattcat ccagcgcatc cccaaccaag
      721 acgactctga cattctttcc tttaaacatc cttacacctc aacaccagcc tttatggctg
      781 catctagttt tttgttttca ttgaaagtcc agactactcc cttgtcgccg tcaactattt
      841 tacctgaata ttcaaggtaa tctattatga ccctcaaagt ctgccgtata gtttttttcg
      901 gcatccttct caaaatctca tttttactca aaggcgtatc cgcttttttt aatatctctt
      961 ccaccatcag caccgtttta aggttcggat agtgaattac ttcttttctt ttgcctgtat
     1021 ctttgacttt ccactctttt ctcttcatca ttcatcctct cttcatcgat cagttcttcg
     1081 ttcattatca actctaaatt tatatactat acaaatttgt atataacctt atatttaagc
     1141 ttttccatta ttttcccgca tcttcttcaa gaaaagaata gcaaagccag ggaatcattc
     1201 acttgtaatt gattgtttat atcatgggtt cttactacat ggcttaaagc tttatcaatc
     1261 gcaaggttta tatatttatt atttttatta tatgttaaat attgttaatt tggtaaaaat
     1321 ggaagagcta tatgcaaaga taaccggctt gtttggagat atagtcagag ggcaagggct
     1381 gaatgattcc ctgcttgcaa tgatccttgc gaggctttat atagagccgg aacctatcgc
     1441 catggacgag cttgccaagg aaaccggcta tagcctggcg tctgtgtgca acaagataaa
     1501 gatgcttggg ccgcttgtaa ctgtgagcaa aaagccaggg tcaaagaaga tatacctctt
     1561 catggataag aatatcatcg gcatctggaa agatatgctt gcaaagaaac agcaagtgct
     1621 tgccagcaca aaggaaaaac tgcccctaat aatcaaggaa tccaggaaaa gggcaaagac
     1681 tgctgatgat aaaaaaagag ttgagatagt cgaaggattt tttgatcaga taagcaaggt
     1741 tgaagggctg ctcaaaaaga tgatggacgg gtttgatgag ctatgaataa tcttgtcata
     1801 gagcttgaag atgtctggaa gatatataag atgggcgaag tagaggttcc tgcgctaaga
     1861 ggacttaacc taaaggtcaa aagaggggaa tttttagcta taatgggtgc aagcgggagc
     1921 ggaaagtcaa ctgctatgaa catgataggc tgcctcgatg ttccgacaaa gggaagagtg
     1981 cttcttgaag gagaagatat atccaaactg agcgagtcgg accttgcaca gataaggggg
     2041 aagaagatag ggtttatctt ccagcagttc aatctcatac ccactttaag cgctgctgag
     2101 aatgtgactc ttcccacgat atttcagaat gtggacaagg agaagaggat gaaaaaggcc
     2161 aaagagctat tagagatggt agggcttggc gacaggataa accacaagcc taacgagctt
     2221 tcaggagggc agcagcagag ggtagcaatt gccaggtccc ttgtgaacga ccctgaactc
     2281 atacttgccg acgagccaac aggcaacctg gatactgcat cgggacataa agtcatagat
     2341 ttccttaaag agatgcatag caaagagaag aaaacaatag ttatggtcac acatgacaga
     2401 gaggtcgcaa gaaacgcaga gaggacagag gtcctttccg gcggccgaat aatacggagg
     2461 gcaaaatgaa aaagataatt gtcgtgctac tattttttgt gcttgcatta tcaagcgttg
     2521 taatgggagc ttcaagcggt cttaattcct attggataaa tgtgaccatg ataaaccaag
     2581 agcctgaccc tgctgaagca ggaaggtatg tcactctaag gttcaaactg gaaaatactg
     2641 ggacagatat agcaaaagat gtcacggtcg agctactaat aaaatatcct ttttcgcttg
     2701 agcctggaga aagtgcaacc aaatatatcg gctctatcag ggcaaggcag atgggtgata
     2761 caggagtaat agtcgattac cgcgtcaggg tggacgagaa ggcgatagaa ggtgaaaatg
     2821 aggtagagct gaggtacaag ctaagcaacc aagattgggt aaaacctgaa gcgtttacgg
     2881 tcgatattgg ggtgcatgac atactgctaa atatcgaaag catcgactcg ccaaggatga
     2941 taaggcccgg cgctataagc aacatcgatg tcaaactaaa aaatcttgct cagagctttg
     3001 tcaaggagat aaaagttacc ttagagctga ctggcatacc gatcgcaact attggagaga
     3061 ccaacaggaa gataatctct cagatggaag gattatctga atcaacagtg cagtttaacc
     3121 tgatggcaga gccagacgca gagtccaagc tttacaaggt tccaataaag cttgagtacc
     3181 gtgataggat cgggaatctg tatatgaaga atgagaccgt agggttgata gttggcgatg
     3241 agcccgatat tgctgcaagc atagacacat caacagtgta ttcaagcggc aggacagggg
     3301 atgtatctgt caaacttgtc aacaaaggtg tgactgacat caaatttatg actgtgaaga
     3361 taggggatgg acaatataaa gtgttgtctg caaaggaagt atatgtaggt aacatagact
     3421 cggatgatta tgaaagcgct gacttcaagc tttctttatc cagggtaaaa ggaaatgagg
     3481 tggcacttcc actgaggctt gaatatgaag atgccaacaa caatgaatat acccaagatg
     3541 tcacggttga aatgccactt tacactgcgt ctgaggcaaa ggcactagga cttagcaagg
     3601 gaagcagcgg agttggcttg tttatcgtgc tcctcatcgt aggtgcaggc atctatgcct
     3661 atatccggat aaagaacaag aagccattaa tacctttcag aaggaaatag tatgcttagg
     3721 gattattttg tcttttcttt tcataacctg aagtcaagaa gacttagaag ctttcttact
     3781 gtgataggga tatttatcgg agttcttgca gtggtaagcc ttgtgagcct tggagaggga
     3841 ctgcgccagg caataatatc ccagtttggg ataagctcga ctgaagtcat atctgtacag
     3901 gctggcggat taagcggata tgggccgcct gggactgctg ttgtaaatcc actgacggaa
     3961 gatgatgccc ttgctatcga gaggataaat tctgtggaaa gggcagtcag caggagcata
     4021 gaaaacatca aggttgaatt caacgatagg gtagacttca cgtatgcgac agacatcccc
     4081 gacggtgatg accgcaagtt tgtatacgca caggtggagc ttgaagctat agaaggcagg
     4141 cttcttaggg aaggggacaa cagacaagtc gctcttggat ataattttgc agaagatgac
     4201 aaatttggaa aaagggtgtt tgtaggcgat aagatacttg taaacaacga ggaatatgga
     4261 gtagttggaa tcactgcaaa gaaaggcagc tttatctttg ataatgttgt ctattttaat
     4321 cgagatgctc tctatgaact gatggaaata gaggatgatg tagatatcat cgcagtgcag
     4381 gtaaaagaca aatcgctaat ccaggaggca gaagaggata ttgagaaact gcttaggaaa
     4441 agaagggatg tcaaagaagg caacgaggat tttgaggtca gcacgcctga ggcgtcactt
     4501 tcatctgtaa accagatatt gcttgctgtc caatcattca tagcgatgat agctttaatc
     4561 tctgttgctg tcggaggcat aggcatagtc aatacgatga ctacatcagt gatggagcgg
     4621 cgaagggaaa tagggataat gaaggcgata ggtgcaagga acagggatat attcatgcag
     4681 ttttttatcg agtctgggct gcttgggctt gtgggagggc tgcttggagt tatcgtcgga
     4741 gttatagtag gactgatagg cacagcaggg ataaacagtt ttgtcggtgc agagtcaacg
     4801 ccgcagataa acctgatact gataatactt acattacttg gcagcttttt gataggctcg
     4861 cttgcaggca taatacctgc actccatgct gcaaagcaaa aaccagtgga cgccctgaga
     4921 tcatgataag cgtcgaaagt gcaaaatatt cattgaggaa tcttatggag agaaaggcaa
     4981 gaagcttcct tacaatactt tctatcttta ttggaatcac aacaattttc atattcgtaa
     5041 gctttgggtg ggggcttttt gactatgttg aatcagtctc ctcatcatcg gctgctgagc
     5101 ttgctttagt gcaggcaagg ggaggggggg cgccgggcct tgcagatgtt tttgcactta
     5161 ctgaagatga cctggaggct gtccagagcg ttaagggagt ctatgaagct acaggggctt
     5221 actttaaggc ggctgaggtc aagcaagggg aaaacagaaa atacgttttt ttaataggat
     5281 atgatcctga tatcccattg ataatgaact cttttaatat agatatagac aaaggcagaa
     5341 aacttgatga tggagatgat ggaaaagtgc ttcttggata taattaccag gtgccaaaca
     5401 agatattccc caaggggtat aagacaggtg attcgatcga ggtgcagggg cagaaactta
     5461 agatagttgg attctttaag ccggtgggca atccccagga tgactcacag atgtatgtca
     5521 tcaatggcta tatagatgaa ctatacgatg aagagcttaa ttatggaatg atcgttgccc
     5581 ggcttgataa aacagacatg aagggaacga tagacaggat aaaaaaagaa gtgaggcaag
     5641 aaagagacct tgaggaagga aaagaggatt tttttgtttc ttcctatgag gatcttatga
     5701 agacttattt ggtcgtgctg aatgtcatag tcggctttat cgtgcttata gggctgatct
     5761 ctgtacttgt cagcgctgta aacactgcaa atacaatgat aacatcagtg cttgaaaggg
     5821 tgagggagat aggggtgata aaatcaatcg gtgcaaggaa caaggagata ttcaagatat
     5881 ttctttttga atcgagcttt cttggatttg tgtctggagt tataggggta ttttttggat
     5941 gggtgataac atttgctgca ggacagcttc ttgatgccgc aggttgggga tttttatcgc
     6001 cgcattactc tttcttgctg ttttttgggt gcataatgtt tgcaacgctg actggtgctg
     6061 tgtctggtgt gatacctgct tataaggcta gcagattaaa gccggtggat gctttacgat
     6121 atgagtaaaa gctttaagct tacaacgagt atcatctaat gctatgaatg gtagggagta
     6181 tgcacttaga catgaatagc taatccatct gagaaatata ttcatctatc ttagccttga
     6241 gtttatcttt caattgccca tgaggaaggg taatctttgg aaccttagta agcgtccctt
     6301 caaatttcat ccctgcctta tgcacttcct gctctatttg tcttgcatat tttgagttga
     6361 actgcttgat agaagggaat tttgttatgg agtttgctcc tgcttttaag agcaggtgaa
     6421 tttcatcaag atgagtgagc catgagccta cgacaatatg gatgtttggg aatgcttgtc
     6481 ttgtgttctt aatccatcgt acatagtaat ctgtatctgg cccttttgtg ttttcaaaga
     6541 cagttccttt ctgaggcttt aggcgataga aggtcagttt gcctacggtg tttttttcaa
     6601 taaatgattt tagattcttg aagtcctcga ttgtttcacc aaggccgaca ataagagtga
     6661 ttgttttttt tagcccgagc ttttctgcaa tcctgaacat ctgctcgatg tcttttaatg
     6721 gttttgaagg gcaaagcttg tccctcaatc ctggggtgat gcactcaact gtcccgcaga
     6781 cgccttctat gtatggcttg aatagattta gctgcttttc tgtcagaatg cccaggttaa
     6841 gccattgctt ttggcctgtt atttcataga tgcccttgat tatttctaag agttccttgt
     6901 ctgtatatgt atcacagccg ccagatataa actctacttt ccagccgcag gctttgcaga
     6961 tcactgcctc tgcgagtatc gatgatagag agcgcctgtc ttttataggg ctttgagggt
     7021 ttgtttttga tgaaagataa cagaaagcgc agtctctttt tgagcagtac cagcttatga
     7081 atattgctct ttcgaaatgg actgattgca tccggcagat gaatggtcaa taggtgtgcg
     7141 gacacttaaa attggttaag tcgcagcgtt ctcaatcaaa gcattatgcc taaggccata
     7201 atggtgctca aaatctccat taatatattc agccctcaga tgcgccatat aatttgcgtt
     7261 ttcttccttc ca
//
LOCUS       DASRUK010000102        11035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2250114, whole genome shotgun sequence.
ACCESSION   DASRUK010000102 DASRUK010000000
VERSION     DASRUK010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 11035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11035
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2250114"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VFF28_04405"
     CDS             <1..291
                     /locus_tag="VFF28_04405"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZX44899.1"
                     /translation="CFEDLLRKYPDKDIYVIVDCWRAHTSFAVRMWSFFHPRFHIVYT
                     PTKSSWMNIIERVFSKLDKDILQNSNFQTVREAMSRISNYFENETRFKTWGS"
     gene            complement(288..1754)
                     /locus_tag="VFF28_04410"
     CDS             complement(288..1754)
                     /locus_tag="VFF28_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44900.1"
                     /translation="MESEEKEFGRVDNKILPKVKEVYEKLSGYINPELIKTEIGYIKD
                     GTGLVMKPVRIGTHNYQIPIPDKKPLHFPDGSVQYAYWYGRDAGRSLFDNNIGPTITQ
                     MKDDALRDIDNMPGISDAQKEARKKVIIQFEKLTRPFLKSYMEGDKYNEMFARQYGIL
                     RNFESHWHKYGPLWNELMNHRPQEVVYFHTYHRVIGKNKAGQSLHTATHPAPGFTKRP
                     NEVDWGLDDRGMPLEVVPAGGHKFVDPFRRVHNLQEGDILMDAFLKYRTETPGAFAIR
                     RVDPSFIGQVDLIEATAYIHCEWDNYRDDYRDGRWHPKSLTVTDYIMAASPSIGGEWQ
                     RKRFPNKIGPEYAHYTMEINGGSFKNVRRATDLNPAFDLRVLKKNQKGQYTGVTQEHR
                     YIGSIRYYAPNEVATEDQLDFALTTRGISLFIIEEISRKGKNLAEIREELISIGKKIA
                     GYDYGPRPFGDPEFTIDHFAIDYARVNEKRTARENKLA"
     gene            complement(1951..3573)
                     /locus_tag="VFF28_04415"
     CDS             complement(1951..3573)
                     /locus_tag="VFF28_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44901.1"
                     /translation="MKAAKSNTTIEDTNRKEIINESSPTVANFMSGLKTLLEDMQKGV
                     QTWNKERESIFDREDNLIVDTDPRIKAIRIIETLGKERIRPNELIMSVHAKLREIETY
                     DRFLATEIGKAIGWQQEENNIPAKALTIIAKYDNDRIFQHFSKYYSYYNEKINASHNP
                     YLSRWSMTLIDYFLSNYNKWMQMIKQIADQSESVKLLSQHKDVRTGVEGLKSLVGNLE
                     KIMDKELKNLSSLEPAIDIAPIYRLINHDEGLAYLGEIVLKIERGKNEGMWEEFRQVQ
                     KAAKQKIPASIETTYGDYETAELFKQWSKPGKGGLHDYPDLWSKEIRKRISEVQRNWP
                     FIFSQGLRTFYLLCQKCQKLADDRFDPDFNRIKEKAKQLIRKEEKAPKDSVKTILRLT
                     QKEKDDIVKEIDKVRKELLAAEKKMMVKVDEFWETVDQFFKESGSDDLQQHCSNAQQK
                     IIQEAATSAELAHRIGSYGFAYDISLGTQLIGRIAEVRAREDDIVKRSSLIAYSILRY
                     GREIVEKKLKADEAIDKSMDMLMKLRGGIRNG"
     gene            complement(3570..5972)
                     /locus_tag="VFF28_04420"
     CDS             complement(3570..5972)
                     /locus_tag="VFF28_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44902.1"
                     /translation="MKNKKLFFLMLAFLAAGSLMASAAWYSNVTSSVQRMPIIPIVVN
                     TVIIWLFFYFLTTLLAAKQLGLESSQGKTVFKVVVTVLAVYFAYQLGNQFVFSQPIVR
                     DFWNYLFDQEGILRSAEKLFVFIGASILITWALRSTLKIGNTYWKIDAFLAVLIAADL
                     ARWGMSKNTFIIFAQVLSTILLAKQFNDVGKKGRITSWFWASFIVIWLSAIMFPEAGI
                     LGIKSIVVWIRSLGLAGHIIMILVFIFGGLIFGAIGKSLMNKEKEAKAKHNWIKNTGQ
                     HLLQRLYNFMNTTRIPMLNTLFRWAGIHNVVLGEPDQEEIGVTIRKIWHELFANMNYL
                     LRLEVYDSKGGYVVGARKGLEQAYTALKEQEGSTYLHLYTESVMNANISRYKMGTGLR
                     GPEIDGSWNVNDGDMGIRKDFWSIDNEGVLKINYSYPFDPGAFTLQNAICSFFFEYIG
                     GIKNFMSSDAPTHQEAQLKLAESWAEKIEQVFNAELARIEKGWSSYQKYLGRRGKLSL
                     IKLEQLRLLLMLRLTGQYKHSYKFARIGAKVYKMNYLKNAAGTSAPKDSSNPPFFIAA
                     GDELFEDNLERTPDAPLDNARRIGAAAKPLVSNEVTEDGYFVDDLNEIRLTGRCSRGY
                     VRRIKPKTIITVNDIEGNPTEIMIDNIITTPMFVTTATWLQDEWTFFFRDVRDGRYHP
                     HSRVALDYLQRHSQERKYNYNNLPRGGRPNATTPAFDREALKDPGRFLYWGKKELQSS
                     ESDMLNCGNIFPAVSTIGMARYLGDFIDTFAQEQERKQKEFFVWDSGREQTLFSGTPQ
                     KK"
     gene            complement(5962..6129)
                     /locus_tag="VFF28_04425"
     CDS             complement(5962..6129)
                     /locus_tag="VFF28_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44903.1"
                     /translation="MKDLPVKIDAELKKRIEEFISKGENRFDFPSVKNFVDKAVLKLL
                     KELEKEGKNEK"
     gene            6255..6377
                     /locus_tag="VFF28_04430"
     CDS             6255..6377
                     /locus_tag="VFF28_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44904.1"
                     /translation="MNKKAYIHHPVVAIIVGFLIGMIVMYLMAKGTIPSPISIC"
     gene            6378..6983
                     /locus_tag="VFF28_04435"
     CDS             6378..6983
                     /locus_tag="VFF28_04435"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZX44905.1"
                     /translation="MYSLNDVEIAYSNYIPSFDFIFALRKQAISELCLKKGDDVLDIA
                     CGAGQNFKHILQKIGQKGSLTAVDYSKAMLGRALYLIGKNGWKNIQLINADAAAVNYK
                     EKYDAIISVLGFSSIPDHKEALKKAVNHLNKGGKLVILDGKLSNFKPLNILMPLLRWN
                     RSWDKTKDIIGDVKKLFPTAKITLNEYKLGSSFIITLRRHN"
     gene            complement(6980..8140)
                     /locus_tag="VFF28_04440"
     CDS             complement(6980..8140)
                     /locus_tag="VFF28_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZX44906.1"
                     /translation="MSNLLDLVNKDLNSHPVAKDPAKAREHAEYGDNELRRRNIEAAL
                     MQFNAAKRLDPENREAQRGFHYVRGINLLLRNDGSAALEHLKKVLAIDPKYRSILNAI
                     RTAAVLADDWQVISQIGIQRSSEATPGSLGYLEAPRQYSDFCTLWPYKIQFEEKKYND
                     HPEVYAYLKEYMGRFISNLPKGMKNCFDGSFFEPPIHLNEMRACIAPVDWKEHREVWL
                     VQCEVDPPIQWVIRPYRTCTRDVETYCYPLKGEHLGMCKEIFLKGRPADLLTLESSCI
                     PEHSSWTGGKLEQVERTFPVLVTVWDVPFYLELHGAFKNEKVGCSSLLQSLTHTVSDV
                     GYTGEAKYRTEKGIAKVLCTPGSPVIDCSPNFLKIRFHQVEAYAYKKDRKWL"
     gene            complement(8150..9274)
                     /locus_tag="VFF28_04445"
     CDS             complement(8150..9274)
                     /locus_tag="VFF28_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44907.1"
                     /translation="MKNLNELIEEDLRGDVARDPAMAKQYIEKANLSLQQSNREMAIM
                     LYTAALRVDPNNREAKIGCNYTRGIDALERGELYSSIACLNEVIALNPHFRSAQDLFR
                     MAVILSNDWFSLGDMGILATDATKPTEEHINRANHIQEVVLSDRTFDGSYKADDCYNL
                     SAKIVLGRQNNILRSRGLENTWGVDTDNHYQTKINLLISENANWKHNLEVWFCIGEKH
                     GQPRIVRPYVNHDQEVANGYLCQRFDEDFLGTLSLCDIANPLIKAYSSTPVDFWVPKK
                     DSIRRFLNPNNPLDYEDVYPIIIHIWEVPMNHKLTVDYKGSVHYQPQGLAKLLTLKCD
                     ESYTGQIQFKGEGGTAWVVSNGRIIMWKVSNPEYTYQASS"
     gene            complement(9276..10040)
                     /locus_tag="VFF28_04450"
     CDS             complement(9276..10040)
                     /locus_tag="VFF28_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44908.1"
                     /translation="MDRNSARALVASMGNAFASGSDQANLGQVLEDSLHPLLKEHIYE
                     LHYRHHGQGSSTDFDRDYGLHEEIVRFFNGIPFGGFSLSDLSRFQRLNQRELQGQELK
                     EKRDKEAEQLRLLERIGQYILGVRAFIDADRARVLAHSYDVNAIRVVDEREVTSIVRA
                     DDSCAEEAVYQNYFRALQARELLTQQQQVAIEGRQNLERSFSPLGTYESGQLTDFLND
                     RDAKYSSVTYATKYCQWRLRQLRDGAITIRVVHRFE"
     gene            complement(10041..10811)
                     /locus_tag="VFF28_04455"
     CDS             complement(10041..10811)
                     /locus_tag="VFF28_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44909.1"
                     /translation="MSIKRWQASADLDRWFNAHSEKEKAAFERKMGICWSQGTPEQKY
                     GAVNAWLEITDREEDHAREDAREARQYEFMTRLEKARIEAFAQALQETSRIIREGHQE
                     DTKRLEQVIARLIENQPPPYTGGGHVMNPFEGRYLPESVTYMDCWRKHAPDEHFPEGR
                     PGPARIVQVIPREEGIHYRCMIAMGSQKPEGFARLGYQIFHEELLRQGDCPRPYSRSI
                     TRRIPLLSWLLVRPDVCPLADAGDYLLGKDGSFNRVSD"
BASE COUNT         2981 a         2513 c         1913 g         3628 t
ORIGIN      
        1 tgctttgaag atttgctccg caaatatcct gataaggata tctatgttat agttgactgc
       61 tggagagcac atacttcttt tgcagtaagg atgtggagct tttttcaccc aagatttcac
      121 atcgtttaca ccccgacaaa atcaagctgg atgaatataa ttgaaagagt tttctcaaaa
      181 ttagacaaag acatcttaca aaacagcaat ttccaaaccg ttagagaagc aatgagcagg
      241 ataagcaact atttcgaaaa cgaaacaaga tttaagactt ggggtagtta agcaagctta
      301 ttctctcttg ctgttctttt ttcatttact cttgcataat ctatggcaaa atgatcgatg
      361 gtgaattccg gatctccaaa gggtcttgga ccatagtcat atcccgcaat tttttttcca
      421 atcgatataa gctcctctct tatttcagca agattcttgc ctttcctcga aatttcctca
      481 ataataaaca ggcttattcc cctagtagta agcgcaaaat ccagctgatc ttctgtagca
      541 acctcattgg gggcataata tcttatgctt cctatgtatc tatgttcttg agtcactccc
      601 gtgtactgtc ccttctgatt tttctttaat accctaagat cgaatgctgg gttcaaatcg
      661 gtagctcttc ttacgttttt gaatgacccg ccattaatct ccattgtata atgcgcgtat
      721 tcgggtccaa ttttgttagg aaatcttttc ctctgccatt ccccaccaat agaaggagaa
      781 gcagccatta tataatcggt tacggttaaa cttttcgggt gccatctccc atctctgtag
      841 tcatccctat aattatccca ttcgcaatgt atataagcgg ttgcttctat taaatcaacc
      901 tgtccaataa atgaaggatc aacccttctt atggcaaatg ctcccggtgt ttctgtcctg
      961 tattttaaga acgcatccat cagtatatct ccttcctgca aattatgcac tcttctgaaa
     1021 ggatccacaa acttgtgtcc gcctgcaggc acaacctcga ggggcatacc cctatcatcc
     1081 agcccccaat ctacctcatt tggccttttt gtaaaacccg gggcagggtg agttgctgta
     1141 tgaagtgatt ggccagcctt gtttttccct atcactcgat gatatgtatg aaaataaaca
     1201 acctcttgcg gcctatggtt cataagttca ttccacagag gcccatattt atgccaatga
     1261 ctctcaaaat tccttaaaat accatactgt ctagcaaaca tttcattata cttgtcgcct
     1321 tccatataac tcttcaaaaa aggccttgtg agcttttcaa attgtatgat aacttttttc
     1381 cttgcttcct tttgtgcatc tgatataccg ggcatgttat caatatctct aagggcatca
     1441 tccttcattt gtgtaatggt agggcctata ttattatcaa aaaggctacg tcctgcatct
     1501 cttccatacc aataagcata ttgcactgag ccatctggaa aatgcaatgg tttcttgtca
     1561 ggaatgggga tctgataatt atgtgtacct atcctaactg gcttcatgac taggccagta
     1621 ccatccttaa tatatccaat ttcagtcttg atgagttcgg ggttgatata tccgcttaat
     1681 ttttcataaa cctcctttac tttgggaagt attttattgt caacacggcc gaactctttt
     1741 tcttcgctct ccatatcctt aagtctgctt atctttgcct gcagtagggg tatgaggaaa
     1801 gagattaaaa aggactttga gggtatgagg gaggactttt tggtcctgta atatctcata
     1861 ctatcataaa ttgttttgtt ccagagccat ttaagtgtat ttgggacagc ttgtcttgct
     1921 ggctttccgc cagtatattc tgcagtatcg tcagccattt cttatacctc ctcttaattt
     1981 cataagcatg tccatgcttt tatcaatagc ctcatcagct ttcaactttt tttcaacaat
     2041 ttctctcccg tatcgaagga tactataggc aatcagggag ctccttttta caatatcgtc
     2101 ttcccttgcc cttacttcag caatccttcc gattaactga gtccctagtg atatatcata
     2161 tgcaaagcca tagctgccta ttctgtgtgc tagttcagcc gaagtggctg cttcttgtat
     2221 gattttttgc tgagcattag aacaatgttg ctgaagatca tcactcccag attccttaaa
     2281 aaattggtct actgtttccc aaaactcatc cacctttacc atcatcttct tctctgcagc
     2341 aagcaactct ttccttacct tatcaatctc tttcacgata tcatctttct ctttttgtgt
     2401 tagcctgagt atggtcttta cagagtcttt tggtgctttt tcctcttttc ttataagttg
     2461 ctttgctttt tctttaatcc tattaaagtc tggatcaaat ctgtcatccg ctagtttttg
     2521 gcatttttgg cataataggt aaaacgtcct caagccttgg gaaaagataa acggccagtt
     2581 cctttgtact tcacttatcc tttttctgat ctcttttgac caaagatcag gatagtcatg
     2641 aaggcctccc tttcctggct tactccattg tttgaagagc tcagctgttt cataatcccc
     2701 atatgtagtt tcaattgaag caggaatttt ctgttttgct gccttctgaa cttgcctgaa
     2761 ttcttcccac attccctcat tctttcccct ttctatcttt aggacaattt cccctaaata
     2821 tgctaaccct tcatcgtgat ttatcaagcg atagatgggc gcaatatcta tagcaggctc
     2881 taaggaggat aaatttttca attctttgtc cattattttc tccaaatttc caacaagtga
     2941 tttgagacct tcaacaccag ttcgaacatc cttgtgttgt gatagcaact ttactgactc
     3001 agattgatca gctatttgtt ttatcatctg catccattta ttatagttgc taaggaagta
     3061 gtcaattaga gtcattgacc atcttgaaag atacggatta tgtgaggcgt taatcttttc
     3121 attataataa ctgtaatatt ttgaaaagtg ctggaagatt ctgtcattgt catattttgc
     3181 tattatcgtt aaggcttttg caggaatatt attttcttcc tgctgccaac ctattgcctt
     3241 gccaatctct gttgctaaga atcgatcata tgtctcaatc tcccttaatt tggcatgaac
     3301 actcattatt agttcatttg gccgtatcct ttctttgcct aatgtttcaa ttattctaat
     3361 agcttttatc cttggatctg tatcgacgat gagattatcc tctctgtcaa aaatactttc
     3421 tctttcctta ttccatgttt gaacgccctt ttgcatatct tcaaggagag tctttaggcc
     3481 tgacatgaaa ttggccactg tgggtgaaga ctcattaata atctcttttc tatttgtatc
     3541 ttctatggtt gtgttgcttt ttgctgcttt catttttttt gcggtgtgcc tgaaaataaa
     3601 gtttgttccc ttcccgagtc ccacacaaag aactcctttt gttttctctc ctgttcttga
     3661 gcaaaggtat caataaaatc gcctaaatac ctggccattc cgattgttga gactgcgggg
     3721 aatatattac cgcagtttag catatcactc tcacttgatt gcaattcctt tttgccccaa
     3781 tacaaaaacc ttcctggatc cttgagagct tccctgtcaa aagcaggggt agttgcgttt
     3841 ggtctgccgc cccttggaag gttattataa ttatacttcc gttcctggga atgcctttgg
     3901 aggtaatcta aagcaaccct gctatgaggg tgataccttc catccctgac atctctgaaa
     3961 aagaatgtcc actcatcttg cagccaggtt gcagttgtta caaacattgg agttgttatt
     4021 atattatcta tcattatttc tgttggatta ccctctatat cattcactgt tataatggtt
     4081 ttgggtttga tcctgcgtac atagcctctg ctgcatctgc ctgttaacct tatctcattt
     4141 agatcatcaa caaaataacc gtcttcagtt acttcgtttg agaccagagg ttttgccgct
     4201 gctccaattc ttcttgcatt atctaaaggt gcatctggag tcctttccag attgtcttca
     4261 aaaagctcat cacctgctgc tatgaagaag ggcggattgg atgagtcctt tggcgctgac
     4321 gttccagcgg cattttttaa ataattcatc ttgtatactt ttgctcctat ccttgcaaac
     4381 ttatagctat gcttatactg gcctgtaagt ctgagcatca aaagtagtct gagctgttca
     4441 agtttaatca agcttaactt cccccttctg ccaaggtatt tttgatatga agaccatcct
     4501 ttttctatac gagcaagttc tgcattgaac acctgctcta ttttctccgc ccaactttct
     4561 gccaatttca attgagcttc ttgatgcgtc ggagcatcgg atgacataaa attctttatt
     4621 ccaccaatgt attcaaaaaa gaatgaacaa attgcatttt gcaatgtaaa agctccagga
     4681 tcaaagggat atgaataatt aatctttaaa actccctcat tgtctataga ccaaaagtct
     4741 tttctgattc ccatatcacc atcgtttaca ttccaggaac catcaatttc tggtcccctt
     4801 agccctgttc ccattttgta cctgctgatg ttagcattca ttacactctc cgtataaaga
     4861 tggaggtatg ttgatccttc ctgttctttc aatgcagtat atgcctgctc aaggcctttt
     4921 ctagcgccaa ctacataacc tcccttcgaa tcgtatactt caagccttaa taagtaattc
     4981 atattggcaa agagttcatg ccaaattttc cttatggtta ctcctatctc ctcctggtca
     5041 ggctctccaa gcacaacgtt atggatgcct gcccaacgga atagtgtatt taacataggg
     5101 attcttgtgg tgttcatgaa attatataat ctttgcagta ggtgttgtcc tgtatttttt
     5161 atccagttat gtttagcctt tgcctctttc tccttgttca tcagagattt tcctatcgct
     5221 ccaaagatca gccctccaaa gatgaacacc agtatcatta ttatgtgccc tgcaagtcca
     5281 aggcttctta tccagacgac tatagacttt attccaagta tgcctgcctc aggaaacatt
     5341 atggcagaaa gccatatgac gatgaacgac gcccagaacc aggaggttat cctccccttc
     5401 ttgcctacgt cgttgaattg ctttgccagc aaaatggtgc taagaacctg ggcaaagatg
     5461 atgaaagtat tttttgacat accccatctt gccaagtcag cagcgataag gacagcaagg
     5521 aaagcatcta tcttccaata agtattgcct atcttgagtg tcgacctcaa tgcccaggtg
     5581 ataaggatac tggcgccgat aaacacaaac agcttctcag cgcttcttaa tattccttct
     5641 tggtcaaaca gataattcca aaagtctctt acaataggtt gcgagaacac aaactggttt
     5701 ccaagttggt atgcaaagta gacagcaagc acagtgacta cgactttaaa tacagttttt
     5761 ccctgggaag actcaagccc aagctgcttt gctgcaagca gggttgtcag gaaataaaaa
     5821 aacaaccaga tgataactgt atttacgact ataggaataa taggcatcct ttgcaccgag
     5881 cttgtgacat tgctgtacca tgcagcactc gccataaggc tgcctgctgc caaaaacgcc
     5941 agcatcagga aaaagagttt tttatttttc atttttgccc tctttttcca gctccttgag
     6001 gagctttaga acagccttgt cgacgaagtt tttgacagat ggaaaatcaa acctattttc
     6061 ccccttagaa ataaattcct ctatcctttt cttaagttca gcgtctatct ttaccggtag
     6121 atccttcaac ttatgctaag ttaccaatag ttactatata aatcttttgg taacaaaaag
     6181 taacttattg aaatgcccct agttttccaa aaagtttaaa tattgaccat tcggtaaaaa
     6241 gcaaaggtga tattatgaac aagaaagctt atatccatca tccggtcgtt gcaataatcg
     6301 ttggattcct gatcggcatg atagtgatgt atctgatggc taaaggaacg attccaagcc
     6361 cgataagcat ctgttagatg tattcattaa atgatgtaga gatagcttat tcgaattata
     6421 ttccttcttt tgattttata ttcgctctaa gaaaacaggc tatctcagag ctgtgtctca
     6481 aaaaaggcga tgatgtactt gatatagctt gcggagcagg ccaaaacttc aaacatatac
     6541 ttcaaaagat aggacaaaaa ggaagcctca cagcagttga ttattccaag gcgatgcttg
     6601 gcagggctct atacctgatt ggaaaaaatg gctggaaaaa tatacagctt atcaatgcag
     6661 acgcagctgc tgtgaactat aaagaaaagt acgatgcaat catatcagtt cttggatttt
     6721 catccatacc tgaccataaa gaagcgctaa aaaaagcagt aaatcactta aataagggcg
     6781 gtaaactggt tatccttgat ggcaaactat ctaatttcaa gccattgaat atattgatgc
     6841 ctcttcttag gtggaatagg agttgggata aaaccaaaga tataatcggc gatgtgaaga
     6901 aactatttcc tacggcaaag attaccttaa atgaatataa gcttggctca agttttatta
     6961 tcactttaag gaggcataat taaagccatt ttcggtcttt cttatatgca tatgcctcga
     7021 cctgatgaaa tcttatttta agaaaatttg gactgcaatc aattacagga gaaccgggag
     7081 tgcacaaaac ctttgctatt cctttttctg tcctatactt cgcttcacca gtgtaaccta
     7141 catcgctaac tgtgtgggta aggctttgta ataatgaact gcaacccacc ttctcatttt
     7201 taaaagctcc atgtagctcg agataaaacg gaacatccca gacagtcact aatacaggaa
     7261 aagtgcgctc tacctgctca agtttacctc ccgtccaaga actgtgttca gggatacaag
     7321 aactttctaa ggtaagaaga tcagctggac ggccctttag aaagatctcc ttacacatac
     7381 ccaaatgttc accttttagt ggatagcaat atgtttctac atcccttgta caagttctat
     7441 agggcctgat tacccattgg ataggggggt ctacttcaca ttgaactaac cagacctccc
     7501 tatgttcttt ccaatctacc ggggcaatac atgctctcat ctcattaaga tggataggag
     7561 gttcaaagaa agaaccatca aagcaatttt tcatcccttt aggaaggttc gatataaatc
     7621 tccccatata ctcttttaaa tatgcataaa cttccggatg atcattatat ttcttttcct
     7681 caaattgtat cttatatggc caaagggtac aaaagtcgct atattgccta ggtgcctcca
     7741 aatatcccaa tgaaccagga gtagcctcac ttgacctttg gattccaatt tgagagatta
     7801 cctgccaatc atcagcaagg acagctgctg tccggatggc atttaatatc gaacgatatt
     7861 ttggatcgat agcaagcact ttctttagat gctctagggc tgcagagcca tcgttcctta
     7921 acaacaaatt tatacctctg acataatgaa atcctctctg agcttcacgg ttttctggat
     7981 ctagtctctt agcggcatta aactgcataa gcgcagcttc aatatttcgt cgtctaagct
     8041 cattatctcc atactcagca tgctctcttg cctttgctgg atccttggct acaggatgac
     8101 tattcaggtc tttgttcaca aggtctagta ggtttgacat attcatcccc tagcttgaag
     8161 cttgataggt atactctgga ttactcactt tccacataat tatccggcca tttgaaacaa
     8221 cccaggctgt tcccccttca cctttaaatt gtatctgtcc tgtataagac tcatcgcatt
     8281 tcaaagtcaa tagttttgcc aacccctgag gctgataatg tacagaacct ttatagtcga
     8341 ctgtcagctt gtggttcata ggtacctccc aaatgtgtat aataatggga taaacatcct
     8401 cataatctag aggattgttg gggtttagaa accttctaat agagtctttc ttaggtaccc
     8461 agaaatctac cggtgttgaa gaatatgctt tgataagagg gttagcaata tcacacagcg
     8521 ataatgtacc taggaagtcc tcatcaaacc tttggcataa atatccattg gcaacttctt
     8581 ggtcatgatt tacatagggc cggacaattc ggggttgccc atgtttttca cctatgcaaa
     8641 accatacttc taaattgtgt ttccagttag cattctctga aatcaacaga ttgatcttgg
     8701 tttgataatg gttatcagta tcaactcccc aggtgttttc caatcccctt gaacgtaaaa
     8761 tattattctg tctacccaaa acaatctttg cgcttaagtt atagcaatca tccgccttat
     8821 agctgccatc aaatgttcgg tctgacagta ccacctcttg tatatgatta gctctattga
     8881 tatgctcttc agtgggcttt gttgcatcag ttgccaaaat acccatatct ccaagtgaaa
     8941 accaatcatt actaagtatc actgccatcc taaacagatc ttgtgccgat ctaaagtgtg
     9001 gatttaatgc tatcacctcg tttaaacaag caattgaaga atataactcg cctctttcca
     9061 aggcatctat ccctcgggtg taattgcatc cgatttttgc ttccctattg ttaggatcta
     9121 ctcttaatgc cgcagtatat agcattatcg ccatctctct attactctgt tgaagagaaa
     9181 ggttcgcttt ttcaatgtac tgcttagcca ttgctggatc tcttgcaaca tctcccctca
     9241 aatcctcctc tattaattca tttagatttt tcatcttact caaacctatg cacgacccta
     9301 atggtaatag ccccatctct tagttgcctt agtctccatt ggcaatactt tgttgcgtaa
     9361 gtgacagaac tatacttagc atctctgtcg ttaagaaaat ccgtaagttg cccggactca
     9421 taagtcccta gtggactaaa tgacctttca aggttttgcc ttccttctat agctacttgt
     9481 tgctgttgag tcaataattc ccttgcttgc aaggctctaa aataattttg atacacagct
     9541 tcctcagcac aactgtcatc agctctgact atgcttgtca cttccctttc atcaaccaca
     9601 cgaatagcat ttacatcata actatgcgcc aaaactctcg cccggtcagc atcgataaac
     9661 gcccttacac ctagaatata ttgccctatt ctctctaaca gccgcaattg ctctgcttct
     9721 ttatctcttt tttccttcag ttcctgcccc tgcaattctc tttgattcaa tcgctgaaac
     9781 ctggatagat cacttagact gaatcctcca aaaggtatcc cattaaagaa cctgacaatc
     9841 tcttcatgta gtccatagtc tctgtcaaag tcagtggatg aaccctggcc atgatgccta
     9901 taatgcaatt catagatatg ttcttttagc aaaggatgta aggaatcctc caatacctgg
     9961 cccaggttag cttggtcgct tcccgatgca aaagcattgc ccatggaggc taccagtgct
    10021 cttgcactgt ttctatccat ctaatcgctc accctattaa atgatccatc cttaccaaga
    10081 agataatctc cagcatccgc taaaggacag acatctggcc ttactaatag ccaacttaaa
    10141 aggggaatcc tccttgtgat gctcctgctg tagggccttg ggcagtcccc ttgccgaagc
    10201 aattcctcat gaaatatctg atagcccaag cgagcaaaac cttcaggctt ttgggaaccc
    10261 atagcaatca tgcatctata atgtataccc tcttctcggg gtatgacctg gacgattcgg
    10321 gcaggaccag gtcttccttc aggaaaatgt tcatcaggag catgcttcct ccaacagtcc
    10381 atataagtta ccgactcagg aagatatctt ccttcaaaag gattcatcac atgaccgcca
    10441 ccggtataag gaggaggttg gttctctatc aaccgtgcaa taacctgctc caaccttttt
    10501 gtatcttctt gatgcccttc acgaattatt cgggaagttt cctgcaaagc ttgtgcaaaa
    10561 gcctctattc ttgctttttc aagccttgtc ataaactcgt actgtctagc ttcacgggca
    10621 tcttcccttg catgatcttc ttctctgtca gttatctcaa gccatgcgtt tacagcgcca
    10681 tacttttgtt caggtgtacc ttgactccag caaatgccca ttttcctttc aaaagcagcc
    10741 ttttcttttt cagaatgagc attgaaccat ctgtccaagt ctgcacttgc ctgccatctt
    10801 ttaatggaca tattaccctc ctaaatacgg ttggctattt aaatatttgc aaaaatactt
    10861 actataaagt aacaatttca aaatttacca ttttgggatt gggctaaaat aggaatgttt
    10921 atatatcagt agattaatta ttgctttatt tctttatcga gtgtaaagag gtgttgccta
    10981 tggctaggaa agcaaggaaa ttagatgttt actgcccaaa tgcatcatgc aagtg
//
LOCUS       DASRUK010000103        10712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1907719, whole genome shotgun sequence.
ACCESSION   DASRUK010000103 DASRUK010000000
VERSION     DASRUK010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 10712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10712
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1907719"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..339)
                     /locus_tag="VFF28_04460"
     CDS             complement(130..339)
                     /locus_tag="VFF28_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HZX44910.1"
                     /translation="MAVKEVLMIGDPKLREKSVDVSDFEETNQVLIDLKDTLEYLQKT
                     KKICRALAAPQIGYQKKVIYMQIVL"
     gene            complement(346..2979)
                     /gene="leuS"
                     /locus_tag="VFF28_04465"
     CDS             complement(346..2979)
                     /gene="leuS"
                     /locus_tag="VFF28_04465"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HZX44911.1"
                     /translation="MADFNKIEKKWQKAWDEAKVFQSKEDKAKKKFYILEMYPYPSAS
                     FLHMGHVRNYTIGDSYARFKRMQGFNVLYPMGFDSFGLPAETASKKEGIHPKKYTENA
                     IKKITEYFKQLGNSYDWSRILASHEPEYYRWNQYFFIKLFEKGLAYRKKAPVNWCDSC
                     SSVLANEEAEGGECWRCASQVVKKELEQWFFKITYYSDRLLEDLDKVDWPDNIKIMQR
                     NWIGKSGGVDIFFPLENSKRVLSTFTTRCDTIHSVTFLAIAPESPLVDELVNGTKYET
                     EVKAFVKKVVKKKIEDRINEEKEKEGAFTGKYAIHPVTKEKLPIYVSNFALMYGTGVV
                     MCDAHDKRDFRFARKYNIPLKLVISKDGKPIEPNEPYVDDGVLFNSGKFSGMHNREAL
                     PKMAEWLENNNMGKRTVNYKIRDWLISRQRYWGTPIPIIYCDTCGTVPVPEEELPVLL
                     PEKVDFKAANPLAKSEEFINTKCPKCGGKAKRETDTMGGFVDSSWYFLRYCDNKNKKE
                     PFANSKVNYWMPVDQYVGGAEHAVMHLLYARFFIKALKDLGFVDFDEPFTRLFNQGIV
                     YKDGAKMSKSKGNIVYQTEISEKYGIDTARLFLMFVSSPDKQMEWSDSGVEGSYRIIN
                     KLFRENEKVKDIALIPRTESKMNAMLRDVEKNIESFDYPKAIISATSFLDHLDTLESI
                     PKQAYENLLKIFSPFCPHVTEELWQMIGNKPFLSLARWPAFDESKIDPRLEAAEEMIN
                     NTIADIANVLKLIKVDKPKKIKLIISPAWKYRFMALIKAELEKTRDVKTLIQACMKEP
                     ELKKHGQDISAMIPALLKDSSKIPSVVLSQEQEFGNLTEAKKQIASQFNSEVEIEKAE
                     QSKEQKAKNASPLKAAIVIIN"
     gene            complement(3013..3501)
                     /locus_tag="VFF28_04470"
     CDS             complement(3013..3501)
                     /locus_tag="VFF28_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44912.1"
                     /translation="MDAIAHLLWTSIIFYGFPNRKTMLLFSILPDLLSWGFYAGYVLK
                     RKLPIGKPNLRDIPRWAHTLYNVTHSIFTFIALSLIVWFITKRPPFIMLPWLLHLMLD
                     IPTHSREYLATPFLWPLSSWVFPGVGWESFKVMVINYIAIISLLAATLIMQQNLLLLF
                     LP"
     gene            3645..6677
                     /locus_tag="VFF28_04475"
     CDS             3645..6677
                     /locus_tag="VFF28_04475"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZX44913.1"
                     /translation="MKRLILLLLVALASFSAIIEGHGSSDVYVRVVSANPVTKTYTFS
                     CNADQQFVRDWFIRPEGEGEEAVILDKPANEFLTYTFKANKLYHIGCIIVDTTDFSKQ
                     YRGDLHIDLSADKPDHKIVPISSNGLSTTLDCQSSISGTPSWQVIDARTGITTSLGSG
                     KTKTYTAPGHGLFDFVCSISGKENGLPVEFFDKGNPYFPDNKGVPHGITNEWIRDVAP
                     SDGSAGNGSTPRGAISGSLQTLPATCQGGVISSDTYDGGRHISCQGGANTLSVSAWEK
                     PDSGEKQYFEMYKKSQIGSGIKICIGTTCLQNDGYAKSSPYSNIPPNGSGTSGPVAPN
                     ASVQRLSSIIASCVGGSIISDTYDGGRHIKCQGSTGSLAISAWEKPDGAVKEYFEMYK
                     QSQAGFGQKICLGNTCIQENGYAKSPLYSTSVPEVPSDGGSSTVLSAPIWLSHTIADN
                     VAPDSFHLEAMEPANPGIHKASEFEIWSASEKVWSLYGATGTLRYHVHTPDGTFSGSL
                     SGMERLEYSTTYNVRARYVDTSNLKTDWSSWRTFTTKAKPTLDPTAPSWTAASGYSVT
                     EFASGFNTPVHVAAAPKIYSNLPLNKRPYLYVTELYGTIKVVYADGSSAVYASGLLNF
                     DPFGSITGGGQMGLTGLYVDESGDLFAGMVYKEGDGVKNKIVKFDTASDGSVYLSTQT
                     IISNLPSAASHQIQQIAKGPDGRLYVGLGDALTPSNAQSDSILAGKIISMKTDGSDVQ
                     VHAKGLRNPFGLDFSASGALYATDNAPDTDDRLIQVIKGENYGWGLNDNFATFHQKRI
                     FTLDVSPVDLENNKGNSDFSGELYVAVAGPIYQQGPQKSKHILEFQMDGSGNILSHRE
                     LVKYTGAGYGTPVGLDFVDNALFFTDIYGEAGFISLGKTDSKIYKVALGAGGAPSGGN
                     QFIAGIEISPWYPKLTGSIVEYEFTCKGIYGSGNYVYDFNYGDGSSISNDLIGKKLHS
                     YPYTNKAYTVSCIVHDKNTGQSRSASMQINPINFIAEK"
     gene            complement(6680..6877)
                     /locus_tag="VFF28_04480"
     CDS             complement(6680..6877)
                     /locus_tag="VFF28_04480"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011153326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit N"
                     /protein_id="HZX44914.1"
                     /translation="MIIPIRCFSCGKPVGHLWEEYNERIKKGEDRKKVMDELGIDKYC
                     CRALFLGHVDLIDIAAQFKKV"
     gene            complement(6882..7283)
                     /locus_tag="VFF28_04485"
     CDS             complement(6882..7283)
                     /locus_tag="VFF28_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011250451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HZX44915.1"
                     /translation="MKPIMSSGKRKRAIARASLKEGKGVVRINNVLLDLYEPKMYRLK
                     LREPLILAGDIVDKINIDVNVFGGGIASQSEASRLAIARALNEYAKSDKLKEKFLKYD
                     RQLLVADIRRHEPSKPNRQGQARAKKQKSYR"
     gene            complement(7280..7699)
                     /locus_tag="VFF28_04490"
     CDS             complement(7280..7699)
                     /locus_tag="VFF28_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HZX44916.1"
                     /translation="MVVFDATDMILGRLASTAARRAILGENVEVINCEKAVITGARKR
                     TLADYKQRFHRGSPTTGPFFRRMPDRFVKRAIRGMIPYKREKGTNALKRVMCYMGVPD
                     HLKDVKAETLPQANISKITVVKYTTVEEICRNMGGKI"
     gene            complement(7701..8069)
                     /locus_tag="VFF28_04495"
     CDS             complement(7701..8069)
                     /locus_tag="VFF28_04495"
                     /inference="COORDINATES: protein motif:HMM:NF028445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18e"
                     /protein_id="HZX44917.1"
                     /translation="MHSKNKKMAKPTGPTNTVLISLIEDLKKKEGNIWKSVASDLSRP
                     SRKRRIVNLSKISRLSKENETILVPGKVLGSGVLEHKITISAYQFSESAKVKLKESGS
                     KILELNDLAKQSPKGIRIIG"
     gene            complement(8070..8170)
                     /locus_tag="VFF28_04500"
     tRNA            complement(join(8070..8115,8131..8170))
                     /locus_tag="VFF28_04500"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8132..8134),aa:Leu,seq:gag)
     gene            complement(8176..8979)
                     /locus_tag="VFF28_04505"
     CDS             complement(8176..8979)
                     /locus_tag="VFF28_04505"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001988.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit D"
                     /protein_id="HZX44918.1"
                     /translation="MIEIKLIDQDKEKNKVSFEMADTTPSFANLLRRAISEEVPVMAI
                     EEVEFRKNSSILYDEMIAHRLGLTSLTTDLKSYNLPSECKCEGKGCARCQLKLTIKAK
                     GPGMVYASEIESKDSAVKPVFPKTPIVSLLKNQALELEATAILGQGKEHVKWSPGLAY
                     YKNKPLIEIDLKKCDACGKCVEQCPTKTINITDNKAVVSKDHLMDCHLCAACIDACPK
                     EAIKITRTNDFIFYLESWGQLDCKKVLLEASSIIDKKLDQFAETLKQAK"
     gene            complement(8976..9371)
                     /locus_tag="VFF28_04510"
     CDS             complement(8976..9371)
                     /locus_tag="VFF28_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015848627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HZX44919.1"
                     /translation="MRPKSRWGIAHIYSSYNNTIIHITDISGTETLALSSGGQVVKSD
                     RMESSPTAAMTAAKKAAEIAMDKGIDSIHVKIKAPGGHNGPNNPGPGAQAAVRALSRM
                     GLKIGIIEDVTPIPHDGCRKKGGKRGRRV"
     gene            complement(9368..9976)
                     /locus_tag="VFF28_04515"
     CDS             complement(9368..9976)
                     /locus_tag="VFF28_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013799006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HZX44920.1"
                     /translation="MGHPKLKSRKFSKPLHPWQKERIEEEKQLLADFGLKNKEEVWKA
                     NSLLSKYAKQAKNLIALTTPQAELTKQQLLSKMNSLGLVNETAKIEDILTITVRDILS
                     RRLETFVHRNGLARTIRQARQLITHEHILVAGEKITSPSYIVSRKEEALIEFAQNSPF
                     FSIDHPERAIKKEMPVGAKKEEKAAVAEKPRRDNRRRREVRK"
     gene            complement(9986..10468)
                     /locus_tag="VFF28_04520"
     CDS             complement(9986..10468)
                     /locus_tag="VFF28_04520"
                     /inference="COORDINATES: protein motif:HMM:NF003140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HZX44921.1"
                     /translation="MEEKKDYKHFIRIANTDLNGARAIITSLRQIKGVSFMFANMVCH
                     LSGVDKYKRTGDLNDDEIKKINDVIVNPKDYKVPSWMLNRRKDLETGQDRHLISADLT
                     FSTDNDIKMQKMIRSYKGVRHILGQPVRGQRTKSNFRKNKGKVHLGVKTRPGAKSGRT
                     "
BASE COUNT         2745 a         2291 c         2162 g         3514 t
ORIGIN      
        1 cgtgcctgga ccatttcata acgtctaagc tcttttgggg taagtagtat ttgctccatt
       61 atatcaccaa acaaggccta agaattactt atatttatag tttactttta ttgtcgtaaa
      121 atttagatgt tatagtacaa tctgcatata gattaccttc ttttggtatc ctatctgagg
      181 agctgccaag gctctgcata ttttcttggt tttttgcagg tactccaatg tatcctttaa
      241 atcgatcaat acctgattgg tctcttcaaa gtcagaaaca tcgacggatt tctcccttag
      301 cttgggatca cctatcatca agacttcttt tactgccatt aagcatcaat ttattataac
      361 aatcgccgcc ttcagcgggc ttgcgttctt tgccttttgc tctttgcttt gctctgcttt
      421 ttcaatctct acttcagaat tgaattggct tgcaatctgt ttctttgctt ctgttaaatt
      481 gccaaattct tgctcttgag acaaaacaac tgaaggtatt tttgatgaat ctttgaggag
      541 cgccggaatc atcgctgaaa tatcctggcc gtgctttttt agctcgggct ctttcataca
      601 ggcttggatc agagttttaa catctcttgt tttttcgagc tcagccttga tcaaggccat
      661 gaacctatat ttccatgctg gcgagattat cagcttaatt ttctttggct tgtcaacttt
      721 gattagctta aggacgtttg ctatgtctgc tatcgtattg tttatcatct cctctgctgc
      781 ctctagtctt gggtctatct tgctttcgtc gaatgctggc catcttgcca aggataagaa
      841 cggtttattg cctatcattt gccaaagctc ttctgtcacg tgagggcaga agggtgagaa
      901 aatttttaac agattttcgt aagcttgctt tggtattgat tctaaagtgt ctagatggtc
      961 tagaaacgag gttgcagaga taatcgcctt tggatagtca aagctctcaa tgtttttttc
     1021 cacgtccctt aacatggcat tcatcttgct ttctgtcctt gggataagcg caatatcttt
     1081 taccttttca ttttctctaa aaagcttgtt tattatcctg tagcttcctt caacaccgct
     1141 gtcgctccat tccatctgct tatctggaga agagacaaac atcaagaaaa gccttgcagt
     1201 atctatgcca tatttttctg agatctctgt ctgataaact atatttccct tgctctttga
     1261 catctttgcg ccgtctttgt aaactatccc ctggttgaac aatcttgtga atggctcgtc
     1321 aaaatcaaca aaccctaaat ccttaagtgc tttgatgaag aatcgtgcat aaagcaagtg
     1381 catgacagca tgttctgctc ctccaacgta ttggtcaaca ggcatccagt aatttacttt
     1441 tgagttagcg aatggttctt ttttgttctt attatcacaa taccttagga aataccagga
     1501 tgagtcgaca aagccgccca tggtgtctgt ctcacgcttt gcctttccac cacattttgg
     1561 gcattttgtg ttgatgaact cttcactttt tgcaagagga tttgcagcct taaaatcaac
     1621 tttttctggc agcaagacag gaagctcttc ttctgggaca gggactgtcc cgcatgtgtc
     1681 gcagtagatg ataggaatcg gtgttcccca gtatctttgg cgggagatca gccaatcacg
     1741 aatcttataa ttaacagttc ttttgcccat gttgttattt tcgagccact ctgccatctt
     1801 tggcaaggct tctctattat gcatgcctga aaatttgcct gagttgaaca atacaccatc
     1861 atctacataa ggctcgtttg gctctattgg tttgccgtct ttgcttatca caagctttag
     1921 agggatattg tattttcttg caaacctaaa atcccttttg tcatgggcat cgcacataac
     1981 tacccctgtg ccgtacatca atgcaaaatt agagacataa atggggagtt tttcttttgt
     2041 cactggatga atcgcatatt tgcctgtaaa agcgccttct ttttcttttt cctcatttat
     2101 cctgtcttca atctttttct tgactacttt cttaacaaat gcttttactt cagtctcata
     2161 ttttgtgccg ttgaccaatt catcaactaa tggactttca ggagctatag ctaggaaggt
     2221 tacagagtgg attgtgtcgc accttgtagt gaaggttgaa aggactcttt ttgagttttc
     2281 caatggaaag aagatgtcta cccccccact ttttcctatc caatttcttt gcataatctt
     2341 gatattatca ggccaatcaa ccttatccaa atcctccaaa agcctgtctg agtagtaagt
     2401 tatcttaaag aaccattgct ctaattcttt ttttactacc tggcttgcac atcgccagca
     2461 ttctcctcct tctgcctctt cgtttgccaa aacagaagaa catgagtcac accagttaac
     2521 aggggctttt tttctatagg caaggccttt ttcaaagagt tttatgaaga aatattggtt
     2581 ccagcggtag tattcaggct catggcttgc aagaatcctt gaccagtcat agctgtttcc
     2641 aagctgcttg aagtattcgg tgatcttttt tatagcattc tctgtatatt tcttagggtg
     2701 gatgccttct ttttttgagg ctgtctctgc tggaaggcca aatgagtcaa aacccatagg
     2761 atacagcacg ttaaatcctt gcatccgctt aaacctggca tagctgtcgc ctatcgtgta
     2821 gttcctgaca tggcccatgt gaagaaagct agcagaagga taaggataca tctctaggat
     2881 gtagaatttt tttttggctt tgtcttcttt tgattgaaag acttttgctt catcccaggc
     2941 tttctgccat tttttttcta tcttattaaa atcagccata attgtctaga aataataacc
     3001 gctttaaaat atttatggta agaataatag caagagattt tgctgcatga tgagcgttgc
     3061 tgcaagcaag cttattatcg cgatataatt tatcaccatc accttaaatg attcccaacc
     3121 tactcctgga aagacccaac tggaaagagg ccaaagaaaa ggggttgcaa gatactctct
     3181 tgagtgagta gggatatcta acatcagatg aagaagccag ggaagcatga taaacggagg
     3241 cctttttgtg atgaaccaaa caatcaggct tagggcgatg aaagtgaata tgctgtgagt
     3301 gacattgtat aatgtatgag cccatcttgg aatatccctt aggtttggct ttcctatcgg
     3361 aagttttctt ttcaagacat agccagcata aaatccccag gataataaat caggcaagat
     3421 ggagaataat agcatagttt ttcggttagg aaaaccatag aagattattg atgtccaaag
     3481 aagatgggcg atagcatcca tttcaagact aatattctga ggtttataaa aatattatgc
     3541 tttgatgatt gctagagcta cttatctcta aaagcaactc tttaatgact ataaagtttt
     3601 ttattagcta aaacccagtt ctaaatgatt ccgggggtgt taaaatgaag agattgattt
     3661 tattattact tgtagctttg gctagctttt cagctatcat agaagggcat ggctcttcag
     3721 atgtttatgt aagggtcgtt tctgcaaatc ctgtgacaaa aacttataca ttcagctgca
     3781 acgctgacca gcagtttgtc agggattggt ttatacgacc agagggtgag ggtgaagagg
     3841 cggtaatcct tgataagcct gcaaatgaat ttttaaccta tacttttaag gcaaataagc
     3901 tctatcatat cggctgcatc atagttgaca ccacagattt ttcaaagcag tataggggtg
     3961 acttgcatat cgatcttagc gcagacaagc ctgatcataa gatagttcct atcagctcaa
     4021 atggattgag tacaaccctt gattgccaat cttctatatc tggcacgcct tcatggcagg
     4081 ttatcgatgc aaggacaggt attactacaa gccttgggag cggcaagaca aagacatata
     4141 ctgcccctgg ccatggtttg tttgattttg tatgttctat ctccggcaag gaaaacggac
     4201 tgcctgtgga attttttgac aaaggaaatc cttattttcc agacaataaa ggtgtccctc
     4261 atgggataac aaatgaatgg ataagggacg tagctcccag cgatggaagt gcgggcaatg
     4321 gaagcacacc cagaggggca attagtggca gtttgcaaac acttccagca acatgtcagg
     4381 gaggggtgat aagctcagat acttatgacg gcggcaggca tattagttgc caaggaggcg
     4441 caaacaccct ttctgtttct gcatgggaaa agcctgacag cggagaaaag cagtattttg
     4501 agatgtataa aaaaagccag attggaagcg gtataaaaat ctgtattggc acaacttgcc
     4561 ttcaaaatga cggctatgca aaaagcagcc cctattcaaa tattcctcca aatgggagcg
     4621 ggacttcagg acctgttgct ccaaatgcca gtgtgcaaag attatcatcg attattgctt
     4681 catgtgttgg tggttcaata atctctgaca cctatgatgg aggaaggcat ataaaatgcc
     4741 agggaagcac tggaagtctt gcaatctctg cttgggaaaa gcctgatggc gcggttaaag
     4801 aatattttga gatgtataaa caaagccagg ctgggtttgg gcagaagata tgccttggaa
     4861 atacctgcat acaagagaat ggatatgcaa aaagtccctt gtattcaaca tctgttccag
     4921 aagtacctag cgatggaggc agcagcactg tgttaagcgc tccaatatgg ctttcacata
     4981 cgattgcaga caatgtcgct ccggattcat ttcatcttga ggcgatggag ccggcaaatc
     5041 ctgggataca taaggcaagt gaatttgaga tatggtctgc gtctgagaaa gtgtggagcc
     5101 tttatggtgc aaccggaacc ttaagatatc atgtgcatac tcctgatgga acttttagcg
     5161 gaagcctttc aggtatggaa aggctggaat actcaactac gtacaatgtc agggcaaggt
     5221 atgtggatac ttcgaaccta aaaactgatt ggtcatcttg gagaacattt acgacaaaag
     5281 caaaaccgac acttgatcct actgcgccaa gctggactgc tgcatctggt tatagcgtca
     5341 ctgagtttgc ctcaggattc aacacgccgg tgcatgtagc tgctgctcct aaaatctatt
     5401 ccaaccttcc tttgaacaaa aggccatatc tgtacgtgac tgagctatat ggcactatca
     5461 aggtggtata tgccgacgga agctcagctg tatatgcaag cggccttctc aattttgacc
     5521 catttggaag cataaccgga ggaggccaga tggggctgac tgggctttat gttgatgagt
     5581 ccggagactt gtttgctggg atggtgtata aagaaggaga tggtgtcaaa aataagatag
     5641 ttaagtttga taccgcaagc gatgggtcag tctacttaag cactcaaact ataatcagca
     5701 atctgccttc tgcagcttcc catcagatcc agcagatagc aaagggtccg gatggaaggt
     5761 tatacgtcgg gcttggggat gcactcacgc caagcaatgc tcaatctgac tccatacttg
     5821 ccggcaagat aatcagcatg aagacagacg gcagtgatgt tcaggtgcat gccaaaggcc
     5881 ttagaaatcc atttggtctt gattttagcg catcaggagc actatatgca actgacaatg
     5941 cacctgatac cgatgaccgt cttatccaag ttatcaaggg tgaaaattac ggctggggac
     6001 tgaatgataa ctttgcgacc ttccatcaaa agaggatatt tactcttgat gtatcaccgg
     6061 tcgatttaga gaacaacaaa ggcaacagcg atttttcagg agagctctat gttgcagtgg
     6121 caggacccat atatcagcaa gggcctcaaa aaagcaagca tatacttgag ttccagatgg
     6181 atggttcagg caatatcctt agccataggg agcttgtgaa atatactgga gcaggatatg
     6241 gaacgcctgt agggcttgat tttgtcgaca atgcgctttt tttcacagat atatatggag
     6301 aagctggatt tatatctctt ggcaagactg attcaaagat atataaggtc gctcttggag
     6361 cgggcggtgc tcccagcggc gggaatcaat ttattgcagg catagagata agcccctggt
     6421 atcctaaact tactggcagc attgttgaat atgaatttac atgcaaagga atctatgggt
     6481 caggcaacta tgtatatgac tttaattatg gggatggctc atccatcagc aatgatctga
     6541 taggaaagaa attgcacagc tatccataca caaataaggc ttatactgta tcttgcatag
     6601 tgcatgacaa gaacacaggg cagagcagaa gcgcttctat gcagataaat ccgataaact
     6661 ttattgcaga aaaataagtt taaacttttt taaattgagc tgcgatatct atcaggtcaa
     6721 catggcccag gaataaagcc ctgcagcaat atttgtcgat gcccagctca tccatcactt
     6781 tcttcctatc ttcgcctttc tttatccttt cattatactc ttcccaaaga tggccaactg
     6841 gcttgccaca tgaaaagcat cgtattggaa taatcattct ttcacctgta tgatttctgt
     6901 ttttttgctc ttgcctggcc ctgcctgttt ggctttgagg gctcatgcct tctgatatct
     6961 gcaactaaca gctgtctgtc gtatttcaaa aatttttcct taagtttatc gctctttgcg
     7021 tattcgttca aggctcttgc gatagcaagc cttgaagctt ctgattggga agcgatgcct
     7081 ccaccaaaaa cattaacatc gatatttatc ttgtcaacaa tgtctcctgc aagtatcaag
     7141 ggctctctta atttcaacct gtacattttt ggctcataga ggtcaagaag gacattgttt
     7201 atccttacca cgccttttcc ttcctttagg gatgctctgg ctatagccct ttttctttta
     7261 cctgaactca tgatcggttt catattttgc cacccatatt cctgcagatc tcttcaacag
     7321 ttgtatattt tacgacagta atcttcgaga tgtttgcctg aggcaatgtt tctgctttaa
     7381 catcttttaa atgatcagga acacccatat aacacataac tcttttgagt gcgtttgtgc
     7441 ctttttccct tttgtatgga atcattcctc ttatcgccct ttttacaaac ctgtcaggca
     7501 tccttctgaa aaatggacct gttgtaggtg agcccctgtg gaatctttgc ttataatctg
     7561 caagtgtcct ttttcttgct ccagttatca ctgctttttc gcagtttata acctctacat
     7621 tctcgcccag tatagctctt cttgcagctg tactagccaa tcttccaagt atcatgtctg
     7681 ttgcgtcgaa aactaccatt ttaacctatt atccttatgc cttttgggct ttgttttgcc
     7741 aaatcgttaa gctccagtat ctttgagcct gactctttta actttacttt tgctgactct
     7801 gaaaactgat aagctgaaat agttatctta tgctcaagga ctcctgagcc aagcactttt
     7861 cctggaacca ggatagtctc gttttccttt gagagcctgc ttatctttga aaggtttact
     7921 atccttcttt ttcttgaagg cctgcttagg tcagaagcaa cacttttcca aatgtttcct
     7981 tcctttttct ttagatcttc aattaatgaa atcagtacag tgtttgtcgg tcctgttggt
     8041 tttgccattt tcttgttttt actgtgcaat gcggggaacg ggatttgaac ccgcgcaggc
     8101 actaagccat acggcgtttg aaggtcattt cctcaaccgt attcttttga ccaggctcag
     8161 acatccccgc agggtttact tagcctgttt aagcgtttca gcaaattgat ctagcttttt
     8221 atctataatc gacgaggctt caagcaagac tttcttgcag tcaagctgtc cccatgattc
     8281 cagataaaat atgaagtcat ttgtccttgt tatctttatc gcctcttttg ggcaagcatc
     8341 gatgcatgct gcacatagat ggcaatccat caaatgatct tttgaaacga ctgctttgtt
     8401 gtcggtaatg ttgatagtct ttgtcgggca ctgctctacg cattttccgc aggcatcgca
     8461 tttctttaag tcaatctcta tcagaggctt gttcttgtag taagcaaggc ctggcgacca
     8521 tttaacatgc tcttttccct gacctaagat tgcagttgcc tcaagctcta aagcctggtt
     8581 tttcagcaag cttacaatag gtgtctttgg gaagactggc tttactgctg aatcttttga
     8641 ctcaatctct gaagcataga ccatacctgg gccttttgcc tttatcgtaa gcttcaactg
     8701 gcatcttgca cagccctttc cttcacattt gcattccgat ggaaggttat atgattttaa
     8761 gtctgtagtc agtgaggtaa gccctaacct atgagctatc atctcatcat ataggataga
     8821 tgaatttttc ctgaactcca cctcttctat tgccataacc gggacttctt cactgattgc
     8881 ccttctcaag agatttgcga atgatggagt ggtatctgcc atctcaaatg aaaccttatt
     8941 cttttctttg tcctggtcaa tcagttttat ttcaatcata cccttcttcc tctttttcct
     9001 ccttttttcc tgcatccgtc atgaggtata ggagttacat cttcgattat gcctatcttc
     9061 aaacccatcc ttgatagtgc ccttactgct gcctgagcac ctgggcctgg gttgtttgga
     9121 ccgttatggc cgcctggagc tttaatcttt acgtgaatgc tgtctattcc cttatccatt
     9181 gcaatctctg ctgctttctt tgctgctgtc atagcagcag taggcgagct ttccatcctg
     9241 tctgacttga cgacctgtcc gccagaggat aatgccaatg tttcagtacc tgaaatatct
     9301 gtaatgtgga taatagtatt gttataactt gagtaaatgt gcgctattcc ccatctgctt
     9361 tttggtctca ttttctaact tcccttcttc ttctgttatc tcttctcggt ttctctgcaa
     9421 ctgctgcctt ttcttccttc tttgctccta caggcatctc cttctttatt gccctttctg
     9481 gatgatctat gctaaagaaa ggtgaatttt gagcaaactc aataagagct tcttcctttc
     9541 ttgaaacaat atatgacggg gaagttattt tctctcctgc aacaagtata tgctcatggg
     9601 ttatgagctg tcttgcctgc cttatggtcc ttgcaaggcc atttctgtga acaaaggtct
     9661 caagccttct gctcaatata tccctgactg taatcgtaag gatgtcctct atctttgcgg
     9721 tttcgtttac caatccgagt gagttcatct tgcttagaag ctgctgcttt gtaagctctg
     9781 cctgaggtgt tgtaagtgcg ataagatttt ttgcctgctt tgcgtatttg cttaggaggg
     9841 agtttgcttt ccaaacctct tccttgttct tcaggccaaa atctgcaaga agctgctttt
     9901 cttcctctat cctttctttt tgccaaggat gaaggggctt gctgaatttc ctgcttttta
     9961 gttttggatg tcccattttt atcgtttaag tccttcctga ttttgctcct ggcctggtct
    10021 ttacgccaag atgaactttt cctttattct tcctgaaatt gctcttggtc ctctggcctc
    10081 ttactggttg gccaaggata tgccttactc ctttgtaaga ccttatcatc ttctgcatct
    10141 taatatcatt atctgtcgag aaggtgaggt ctgcggagat caggtgcctg tcttggccgg
    10201 tctctaagtc ttttctcctg ttaagcatcc atgatggaac cttataatcc ttagggttta
    10261 cgatgacatc gtttatcttc tttatctcat catcgtttaa gtctcctgtc cttttatatt
    10321 tgtcaacgcc ggataaatgg cagaccatgt ttgcaaacat aaagctaacg ccttttatct
    10381 gccttagaga tgtaataata gctcgcgcac cgttcaaatc ggtgtttgct atcctgataa
    10441 agtgcttata atcttttttt tcttccatag taaaccgcta gaatccaacc tttataaccc
    10501 taaaggcgct ctgattgaac tcgggctgaa ataataggaa atgtcggtta tttaaaaatg
    10561 tttcgacttg gagtaatcgt ttagctttat cccactaatt ctaattatat acttgagcac
    10621 cgaaatattt atttagttct tgtagttaca ttgtaactac atgagaaaac caattcaaat
    10681 gaagatggaa ggttacgaag ttgttgagaa ga
//
LOCUS       DASRUK010000104         5902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1601024, whole genome shotgun sequence.
ACCESSION   DASRUK010000104 DASRUK010000000
VERSION     DASRUK010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601024"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VFF28_04525"
     CDS             <1..739
                     /locus_tag="VFF28_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44922.1"
                     /translation="VSYLTFIGLGKRASTHRFFWHLYLAFFLALAIYQMVKFMIKEID
                     IRHSFALGCLILMIIAYPLVPKTKTGQGVMDPYNWEGLKWVSENIPKNASIYYMYSDP
                     LSQAAALYNSKHLAFKVSTDEFIEGIKSQAIQRKFTFGLADSYSAYLCHKGLWSFGYY
                     LLSPKPENYECADKYQPLEGWQSPEKDICSLEYIYFNKITYQPVMGQYSQAIKELMLK
                     KGWIKEIFSNEVVSIIENDRPGVECLE"
     gene            708..1136
                     /locus_tag="VFF28_04530"
     CDS             708..1136
                     /locus_tag="VFF28_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44923.1"
                     /translation="MIGQGLNALNKELAGKLKKESVHLITLFFTFIIIFKLLFAKESF
                     GLIISYVFFFFWLFILPGFSLMYYWHEELDFIERLIIGTCLGLAITGIVGYNLGLIGI
                     KMQLQGIILPLLCLALASFLILKKSSDTVKKDETGNDAKV"
     gene            complement(1363..2934)
                     /locus_tag="VFF28_04535"
     CDS             complement(1363..2934)
                     /locus_tag="VFF28_04535"
                     /inference="COORDINATES: protein motif:HMM:NF042625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6541 family protein"
                     /protein_id="HZX44924.1"
                     /translation="MKFTKGTEAIIIALIYLVGLYIWTLPIQKDKMPYGEFDAISHWE
                     LGDYMAQADLPIVTLPAYIDRRYGRDNAFKPHTLWYHPPYHTNFAVAQVLGGDRIVPI
                     YLLNAMLSSFLVLALYFFMRKMFGFLPALLSSVLAAFSARDIFVFLWGQWPERISYYM
                     IPLVLYAYYKYTDSYLSGQTKQIYIYLTGILLGITIFLHPMGFFYSSTVLVVYSLFLL
                     ARKRRIFFNWKHLTIATIFFILLLSLFPYQTGSVVVQFFGKKEGIDTSANDFSRLFSW
                     FKKPNKDLGVPDFYFSFRQMHGLWTLPFLLLGIAFALWKRRDKDYVLLAWLIGLYIVL
                     HMDVIGKGPFVHRTLSASSHIFIPFTVLGAVYISSFTSNHHYKIYIKYAIAVLVIILA
                     VSVNGKFAFSTLKDAYSGLGRMNTAQYDAAEWMRQNLPQDANVTDIGVINLQRAYWIQ
                     GVSLHMQTPFAPKTENFPTEEEMAQYIMIDYSDLLRMGRRDIAEQILAWERDAMANDT
                     IVYNKDDVRVYKNEE"
     gene            complement(2931..4130)
                     /locus_tag="VFF28_04540"
     CDS             complement(2931..4130)
                     /locus_tag="VFF28_04540"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HZX44925.1"
                     /translation="MRWIDFWVGIPICFALSIINWPIKYFRKQQKPKKILFLELSEMG
                     STMLAYSAMKKAKERFGAELFFMIFKENEASIRLLDIIPDENVITIRSKSFGVFFTDI
                     IHAIFKMRSLKIDTVIDLELFSRFTAILSFLSGARQKVGFFKYHMEGLYRGNFLTNKV
                     TYNPHIHISENFMALVYSLEEDTSVKPLLKRAIKKEEIYTAKIRSSDEEKRIILQKIQ
                     ELNPGFSSSSKIILLNPNASQLLPIRRWPIKNFCELAKMTLEHDKKAFVLITGTQAEK
                     PDAKEICDYAQSKRCIDFTGRTSFKELIDLYNVSRILVTNDSGPAHFASLTEISIIAI
                     FGPETPHLYSPLSKNCTCVYSNYACSPCVSAFNHRKTPCTESKCLEAIRPEEVFRILR
                     QQLNSSP"
     gene            complement(4142..5041)
                     /locus_tag="VFF28_04545"
     CDS             complement(4142..5041)
                     /locus_tag="VFF28_04545"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HZX44926.1"
                     /translation="MKALVTGINGFVGPYLKDHLLKNGIQVFGMDINQGADVNFTCDL
                     LDRQAIFEAVKSSRPDLLFHLAGQSSDKLSFAKPELTFNVNVLGTKNILDAVRASSPD
                     CRILIVSSANIYGIPVKVPLTEQSRVSPISPYGESKLKQEELTLSYGLSSVIVRTFPH
                     TGPGQIPIFVCSDLAKQAAEAEAGKRDEICVGNTQVERDFSDVRDIVRAYLLAVQKCR
                     CNEIYNVCSGKSYSVGNVFEILKSLISKDIKLLEKKEKKRERDILILSGDNSKFVKQT
                     GWKPEISFEKTLGDLLDYWRQRI"
     gene            complement(5038..>5902)
                     /locus_tag="VFF28_04550"
     CDS             complement(5038..>5902)
                     /locus_tag="VFF28_04550"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003350021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HZX44927.1"
                     /translation="RRSSVETFERIEHIKDNIHLICGDLTDQCSLNETVRKVMPEEVY
                     NLAAQSFVPESWNQPILTGDVTALGTLRILDAIRHIDPSIKFYQASSSEMFGKVQEIP
                     QSEKTPFYPRSPYGVAKVYSYWMAKNYRESYGMFCANGILFNHESPRRGKQFVTRKVT
                     DSVAKIKLGLQKELVLGNLQSKRDWGFAKDYMEAIWLMLQQDKADDFVIATGETHTVK
                     ELCEVAFDYVGLDCDKFLKTDKKFLRPAEVDLLVGDYSKAKRVLGWEPKVKFKQLVEM
                     MVEEDLKRHAK"
BASE COUNT         1705 a         1308 c         1167 g         1722 t
ORIGIN      
        1 agttagctac ctaacattca taggtcttgg aaaacgagca tcgacgcata ggtttttctg
       61 gcatctttac ctggctttct tccttgcgct ggcaatatac cagatggtaa agttcatgat
      121 aaaggagatc gatattaggc atagctttgc cttaggctgt ttgatactca tgatcatcgc
      181 atatccttta gtccctaaaa caaaaacagg acaaggagtg atggatccat ataattggga
      241 aggcttgaag tgggtttccg agaatatacc aaaaaatgca agcatctatt acatgtattc
      301 tgatcccttg tcccaggcag ctgccctata taactctaag catcttgcct tcaaggtcag
      361 cactgatgag ttcatcgagg gtataaaatc ccaagcgatc cagagaaaat tcacttttgg
      421 ccttgcagac tcttattccg cttatctttg ccataagggc ttatggtcat ttggatatta
      481 cctgctaagc cccaagcctg agaattatga atgtgcagat aagtaccagc ctttagaagg
      541 ctggcagtcg cctgaaaagg atatctgcag cctggaatat atctatttca ataagatcac
      601 ataccagccg gtcatgggcc agtacagcca ggcgataaag gagcttatgt taaaaaaagg
      661 atggatcaaa gagatatttt caaacgaagt cgtctcaata atagagaatg ataggccagg
      721 ggttgaatgc cttgaataaa gaactggcag gtaaattaaa gaaagaatca gtacatctca
      781 tcaccttatt ctttacgttt atcataatct tcaagctgtt gttcgcaaag gaaagttttg
      841 gcctaatcat tagctatgtg ttcttttttt tctggctttt tatcctgcca ggtttcagct
      901 tgatgtatta ttggcatgaa gagcttgatt tcatcgaacg cctcatcata ggcacctgcc
      961 tgggattagc aataacaggt atcgttggct acaaccttgg cctgatagga ataaagatgc
     1021 aattacaagg gataatcctt cctctgttat gccttgctct tgcttctttt cttatattga
     1081 aaaaaagctc cgataccgta aagaaggatg aaactggcaa cgatgccaag gtttaatgat
     1141 ttaaatagca agcccaggta atagacaaaa acagagcaaa gccctattcc cagaactgct
     1201 gaaaatatca acttctcact gagatcaagc tcataatagt caagcatcag atagaaaggg
     1261 acaataaaaa acatggcaag accgactatc gtccgcacgc ctactatccc gaataataga
     1321 taaaaggaag cgactgaaac caaaataata actatcagag atttattctt cattcttata
     1381 aaccctcaca tcatccttgt tatatacaat tgtatcgttt gccatagcat ccctttccca
     1441 agcaaggatc tgctcggcaa tatctcttct tcccatcctt aataggtcag aatagtcaat
     1501 cattatatac tgcgccatct cttcttcagt ggggaaattt tcagtctttg gcgcaaaagg
     1561 tgtttgcata tgcagtgata ctccctgtat ccaataagcc ctttgcaaat taatcactcc
     1621 tatatcggta acattggcat cttgaggcag attttgtctc atccactctg ctgcatcata
     1681 ttgagcagtg ttcatccttc ccagacctga ataagcatct tttaatgttg aaaatgcaaa
     1741 ctttccatta acgcttactg ccagtatgat gacaaggacg gcaatcgcat atttgatata
     1801 aatcttatag tgatggttgc tggtgaagga actaatgtag actgctccca ggacagtgaa
     1861 gggtataaag atatgcgatg atgcagaaag cgtcctatgc acaaaaggac ccttgccgat
     1921 aacatccatg tgcaaaacaa tatataatcc tataagccat gcaagcaaaa catagtcctt
     1981 gtcccttcgc ttccataatg caaatgctat tcccaaaagc aggaaaggca gcgtccaaag
     2041 gccatgcatc tgcctgaaag agaaatagaa atcagggacg cccaagtcct tgttcggctt
     2101 cttaaaccat gaaaaaagcc ttgaaaaatc attggcagag gtgtctatac cttctttttt
     2161 accgaagaat tggactacca ctgatcctgt ctgataggga aatagcgata acagtaaaat
     2221 gaaaaaaatc gttgctatag taagatgttt ccaattaaaa aagatccttc ttttcctcgc
     2281 aagcaggaat aatgaataaa ccacaagaac cgttgaagaa tagaagaaac ccatcggatg
     2341 aagaaatata gttatgccta gcaggattcc tgtcagatag atatatatct gctttgtctg
     2401 gccgcttagg taggaatcag tatatttgta ataagcatac agcacaagcg ggatcatata
     2461 atagcttatt ctctcaggcc attgacccca taagaacaca aatatatccc ttgctgaaaa
     2521 agcagcaaga actgatgaaa gaagagcagg aagaaagcca aacatctttc tcatgaaaaa
     2581 gtacagtgca agcacaagaa aagagctgag catcgcattg agaagataga taggcactat
     2641 cctatcacca ccaaggacct gggcaactgc gaagttggta tgatatggag gatgatacca
     2701 tagagtatga ggcttaaatg cattatccct cccataccgc ctatctatat aagccggcaa
     2761 ggtgacaatc ggaagatctg cctgtgccat ataatctccc aattcccagt gagatattgc
     2821 atcaaactca ccgtaaggca tcttatcttt ctggataggc agagtccaga tataaaggcc
     2881 aacaagataa attaaagcaa tgattattgc ctctgttcct ttggtgaact tcatggtgac
     2941 gaattaagct gctgccttag tatcctaaaa acctcttccg gccttatcgc ctcaaggcat
     3001 ttgctctctg tgcacggggt cttgcggtgg ttgaaggctg agacgcatgg agagcatgca
     3061 tagtttgagt agacacatgt gcagttcttg cttaacggtg agtaaagatg aggcgtttca
     3121 ggcccaaata tagcgataat ggaaatttca gtcaaggatg cgaaatgagc tgggccgctg
     3181 tcatttgtta ccaggatcct tgaaacattg taaaggtcga taagctcctt aaagctggtc
     3241 ctgccggtaa aatctatgca tcttttactt tgggcataat cacaaatctc ctttgcatca
     3301 ggcttttcgg cttgcgttcc tgttatcagc acaaaagcct tcttatcatg ctccagcgtc
     3361 atctttgcaa gttcgcagaa attctttatc ggccatctcc tgatgggcag cagttggctg
     3421 gcgttaggat taagcaggat aatttttgaa ctactgctaa atcctggatt tagctcttgg
     3481 atcttctgga gaataattct cttttcttca tcgcttgacc tgattttggc tgtataaatc
     3541 tcttctttct ttatcgctct tttaagcaat ggcttgactg aggtatcctc ctccagcgag
     3601 tagaccagcg ccataaaatt ttcgcttatg tggatgtgcg gattataggt cactttattg
     3661 gtaagaaaat tgccccggta aagaccttcc atatggtatt tgaagaagcc aactttttgc
     3721 cttgcccctg agagaaaact taagatagct gtaaaccttg agaataattc aagatcgata
     3781 accgtgtcta tctttaaaga tctcatctta aatattgcat gtatgatgtc tgtgaagaat
     3841 acgccaaaag atttgctcct gatagtgatc acattttcat ctggaattat gtcaaggagc
     3901 cgtatgcttg cttcattttc cttaaatatc atgaaaaata gctctgctcc aaaacgttcc
     3961 tttgctttct tcatagcgct gtaagcaagc atcgtgcttc ccatctctga cagctccaga
     4021 aacaatatct tctttggctt ttgctgcttt ctaaaatact taatcggcca attgattata
     4081 gagagagcaa agcatatagg gatacctacc caaaaatcaa tccatctcat gaacggcact
     4141 ttcatatgcg ctgtctccaa taatccagca gatcgccgag tgttttttca aaagatatct
     4201 ctggcttcca tcctgtttgc tttacaaatt ttgagttatc gcctgataat atgaggatat
     4261 ccctttccct cttcttttct tttttttcca gcaatttgat gtccttactt atcaaggact
     4321 ttagtatctc aaaaacattg cctacactat atgattttcc gctgcagacg ttataaatct
     4381 cattgcacct gcatttttga acagcaagga gataggccct tacaatatcc ctgacgtcgc
     4441 taaaatctct ctcgacttgg gtgttgccga cgcatatctc atctctcttg ccggcctcag
     4501 cctctgcggc ctgttttgca aggtcactgc atacgaaaat ggggatttgt cccggcccgg
     4561 tatgaggaaa agtccttacg atcactgagc ttagaccata ggaaagtgtc agctcttcct
     4621 gttttagctt tgattcacca taagggctga tcggacttac tctactttgc tcagtcagag
     4681 gcacctttac aggaataccg tagatgtttg cagagctcac tatcagtatc ctacagtccg
     4741 gcgagcttgc cctcactgca tcaaggatat tttttgtgcc aagtacattg acattgaaag
     4801 taagctcggg ctttgcaaag ctgagcttat cagaggactg gccggcaaga tggaaaagca
     4861 agtccggcct tgagctttta acagcttcaa agatagcttg cctgtcaaga agatcgcaag
     4921 tgaagtttac atcagcacct tgatttatat ccatgccaaa aacttgtatg ccattcttca
     4981 atagatggtc tttcagataa gggccgacaa atccgttgat gcctgtaacc agggctttca
     5041 tttcgcatgc cttttcaaat cttcctcaac catcatttca accagctgct tgaatttaac
     5101 ctttggctcc catccaagca ctctttttgc ttttgagtag tcgccgacga gaagatcaac
     5161 ctctgcaggc cttaaaaatt tcttatctgt cttaaggaac ttatcacaat caagcccgac
     5221 ataatcgaag gccacctcgc acagctcttt tactgtatga gtctctcccg tagcaataac
     5281 aaaatcatca gctttgtcct gctggagcat cagccatata gcctccatat agtcctttgc
     5341 aaagccccag tcccttttag actgaagatt gccaagaacc agctcttttt gaagcccaag
     5401 ctttatcttc gccactgaat ctgtgacctt tcttgtgaca aactgcttgc ctcttcttgg
     5461 tgattcgtgg ttgaacagga tcccgtttgc acagaacata ccatagctct ccctgtaatt
     5521 ctttgccatc cagtatgaat atacttttgc gacgccatat gggcttcttg gataaaaagg
     5581 agttttttcc gactgaggta tctcttggac cttgccaaac atctcgcttg atgatgcctg
     5641 atagaacttg atcgatggat cgatatgcct tatagcatca aggatcctaa gggtaccaag
     5701 ggcagtcaca tcgccagtaa ggatcggctg gttccaggat tcagggacaa agctttgggc
     5761 agcaaggtta tacacttcct caggcatcac ctttctcaca gtttcgttta aagagcattg
     5821 gtctgttagg tcgccgcata tgagatggat attgtcctta atatgttcga tacgttcaaa
     5881 agtctcgacg ctcgatcttc ta
//
LOCUS       DASRUK010000105        10504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_748388, whole genome shotgun sequence.
ACCESSION   DASRUK010000105 DASRUK010000000
VERSION     DASRUK010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 10504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10504
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748388"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..423
                     /locus_tag="VFF28_04555"
     CDS             91..423
                     /locus_tag="VFF28_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44928.1"
                     /translation="MSYRPITIEKVETLKCLGCFDIYIRGEPSRQATKVKLRNWKITW
                     DLQACSTDSDCIPEPECHPLNCINKKFEGDYKKPEACTEIFMYEAAYKEEDCACISNR
                     CENKIDII"
     gene            921..1964
                     /locus_tag="VFF28_04560"
     CDS             921..1964
                     /locus_tag="VFF28_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44929.1"
                     /translation="MNRTAIEFPFVYNEDLAFLGEAIRVEGHIKSNFYQSTISNKNLA
                     FLDMIKKVLVRFVPSSSVYETYSIEADIPDKEMKLINSHMNCSRYKASRSNKFKIRFY
                     DNIERFPKTYHLTFDSGFNLTISISIIGDELVGTSDYGIVSPVRTLALTNITFNRILN
                     IFLDIPCGKKSDIIKIPLLLMDSPPTVKIAAINAVLCSESGVEDKRVRILMNSRHYID
                     DLRVLFIDLGIPVLVRETCLSITGRKNLQKVAESLNFISTKKQKKMKTNLLSYKRDVF
                     QKGEALKEILLALKEKDYTTLELSQELNKHKDTIQHHLQGGIKENFIKKDSISWPYKY
                     SITPEGSEYLGRC"
     gene            1965..2961
                     /gene="kae1"
                     /locus_tag="VFF28_04565"
                     /pseudo
     CDS             1965..2961
                     /gene="kae1"
                     /locus_tag="VFF28_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010886049.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0061711 - N(6)-L-threonylcarbamoyladenine
                     synthase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex N(6)-L-threonylcarbamoyladenine
                     synthase Kae1"
     gene            2987..3709
                     /locus_tag="VFF28_04570"
     CDS             2987..3709
                     /locus_tag="VFF28_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44930.1"
                     /translation="MHSRQNLDIKNLEIVITNEGRTKADIKLSVSGINKYLFLSEQSL
                     TLEPGESAEVELNFAGKDIGAFIGSLIVADQAIPILLIVEEENVLFDVSIDLKNPGIP
                     QGKDIQSRITLKSMGQQKKVDVYLTYIIKDSEDNIVLSEYETLAVEKELVYDKAFDTK
                     NLPKGDYMASVEAEYLGQKAISGALFSVFAQEMPSLPIKANYKPIILLLIAAALILFR
                     KKAVILLLTMGSRRKTKKPRKH"
     gene            complement(3706..5007)
                     /gene="lonB"
                     /locus_tag="VFF28_04575"
                     /pseudo
     CDS             complement(3706..5007)
                     /gene="lonB"
                     /locus_tag="VFF28_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011011584.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease LonB"
     gene            complement(5064..7109)
                     /locus_tag="VFF28_04580"
     CDS             complement(5064..7109)
                     /locus_tag="VFF28_04580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZX44931.1"
                     /translation="MLKFKTTADIKIPKNIIDQVIGQEAAVNIMKKAAQQRRHVLLIG
                     EPGTGKSMLGLGLAELLPKEKLVDILSFPNPNDENSPLIRTVPGGQGRELVAKSRISS
                     MGLFKNQNILMFVLLIIAMIAPWWALNHYTKELGQTAGAIIFTAFFLGGMLFLASFLI
                     FLNVGKRMAAPQVRIPKVIVDNFNKELAPFNDATGTHAGALLGDVLHDPFQTFFTEQL
                     LQIITNNGVQPYKINQTVDSLMEKHSDNILRKGNNYEAVFLPKNELFVLGETKGCAST
                     AEVLSCNRYDYDGKMIKLTTSENRELTVTPEHQIAVLDNGKISYTEARNIKEGTELWA
                     NDIIIDEQDIINTYDLRQQEQCRLYHQYLDIKAKHPGWGYKRIAAAMNQPYEKTRRWH
                     AKKHYPVPLQTADWLKKKGLLPLKIDNPKLPLIAKILGATYGDGGIFDNLNGIFLSSS
                     EIEAVSEFRKDLEGVFQISGNSRIIEGGEKGHSWCCQITNRNIIRLFLALGAPRGNKT
                     KLKLKVPEWIKLRQELEAEFYGSLLGGELGTPIIHKRGNYLTSLEVGITGSPDLKSNR
                     LSFLGEVVDFLKRNRVNVTSIYEGRTNRDDSLIYRMLIEKKMDNVILFMMSIKLNYCR
                     YKTDRLYLALGQWAALKRDKFNELTKKGYGAERAMDVLNLSPNSLYLILNNYPEAAI"
     gene            7161..7514
                     /locus_tag="VFF28_04585"
     CDS             7161..7514
                     /locus_tag="VFF28_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44932.1"
                     /translation="MGVELKDKTLLKIAITGSLVGIALLILLSQFYQVGETSIIDIKD
                     GLIGSDVKVEGTVNSIFRTNSTSIFTLSSPSELKIMASTNLSISEGDYVEVIGQIDEY
                     KGEKEIIADRIRKIE"
     gene            complement(7517..10243)
                     /locus_tag="VFF28_04590"
     CDS             complement(7517..10243)
                     /locus_tag="VFF28_04590"
                     /inference="COORDINATES: protein motif:HMM:NF014565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004576 - oligosaccharyl transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STT3 domain-containing protein"
                     /protein_id="HZX44933.1"
                     /translation="MENLKEKLLSKSWILLLLIPLVITAVVRLEPMKLAPLDGSAQSS
                     VNAYFKEQISLQIANQFPNLPAQERVKLADREYAKFVGENKQLITDQIKANSEGFKSR
                     LQYTSGDSTYVYLGDIDSYYWLRMSRNIIEKGTQCDDETDGKCYDTYTVAPLKNEKPI
                     DFYPIVIVWVYKFFKIFNKDMSLMQASFLTPLVLSLLLSIPLFLAIRKVAGNIGAVVG
                     TTLVNVNPFVLTRSLGSDSDILNILFQAIFLWLAFEFFYAANPKMKYTWGALAGACLA
                     LYSKFWSGWWYLFDLFILAVVVKMAYIYIKKMMKKSEGGLPIKENVVSVSIFVASVFI
                     FYTLLSGDIGAFFSMLGSQFDVLRFKHASNLNLWPNVLTTVAEFNNMSIGDIVPSFRS
                     ILRIPVFLMVLFGITLLSFKNIKNKLPLFLGVLAFDVIAYYIIVNASNPLTIFIALIP
                     LAIGLYANLKEEGDYHPDAIFMLTMLICLVTYFTTTGIRFLFLMAIPVYVLVSIFFGK
                     CLSLLVSTLRKNLKIHGAAFQAFSILAAIWFLMTPVQAGISAAQQYIPNVTDEWVASL
                     EKIRDESKPDAIINSWWDFGHWFKYYADRRVTLDGSSQNNPQLHWLGKLLLTSDENTS
                     VGILRMLDCGGNNAFDEINKVLGDIPHSIDVLNKIITVPEKEARQILKEHKLAENDIE
                     KVIPLTHCKAPENFLITSQDMIGKAGVWAHFGSWDFKKSYINSAVDSRFEQEIIDKFK
                     EYDVEEQITRKYIDERRGLGSQESINAWIAPWPGFITGKMQCPLTQDIAECGFSQQGG
                     TIPVKIDFAKKEGYIQKSDGSKQNLARIGLVDNGKFEIIKSEGDAAGLGITVVKDKDA
                     AYAVFSSPELAGSMFSRLFFFDGAGTKQFDRFYDTRSVLGERIITWKVKWVE"
BASE COUNT         3054 a         2207 c         2151 g         3092 t
ORIGIN      
        1 tattcatagg atgaacatgt cagcgcatgc ataaggccat gggagctgga tgaatcaaaa
       61 aaagaggcag caaagatagt tgtcttgcct atgtcttatc gtccgataac tatcgaaaag
      121 gtcgagacct tgaagtgcct aggctgcttt gatatctata tcagaggaga gccaagcagg
      181 caggctacaa aggttaaact aagaaattgg aagataacct gggatcttca ggcttgcagc
      241 acggattctg actgtatccc agagccagaa tgccatccgt tgaactgcat caacaagaag
      301 tttgaaggcg attacaaaaa gccagaagct tgcactgaga tattcatgta cgaggcagcg
      361 tacaaggagg aggattgtgc gtgcataagc aacaggtgtg agaacaagat agacataata
      421 tgaatccaga gtttgcaaaa gacatcggcg ctctagcctg cgtttgttag agacaaagac
      481 agaagaaata aatcgggaga taccctggag caaaggaata agtcacttgc cacttgccat
      541 gactgtgcat ggggtattta aacacggatt attccccatt attgatggta gcttataatt
      601 cattaaatct ttctttgctt ccaagccatg aattttgcgt tttactttcc ggatcaaggc
      661 aaagggactt aatagaatta gctgcaaata tattatctga aggtaggcaa tggggtaaat
      721 ataaacgatt ggctttttat ctatccaaaa aagtgatcag tcttcaggat attagctgta
      781 attcaatagc cgataattat attcctaatt ggaaaaccaa taagaagttt cttcccttag
      841 attgttatta taaactatcc actttaacag gcattaaatc ccatcctcag gacatctcgc
      901 ttgttaaatg caaagcagca ttgaatagga cagcaattga atttccgttt gtctacaatg
      961 aggatttagc attccttgga gaagcgatta gagtagaagg gcatattaag agcaactttt
     1021 atcaatctac catatcgaat aaaaatcttg cttttctcga tatgataaaa aaagtacttg
     1081 taaggtttgt gccctctagc tcggtatatg aaacctacag catagaggca gatataccgg
     1141 ataaagagat gaagttgatc aatagccata tgaactgcag cagatataag gcgagtagga
     1201 gtaataaatt taagattcga ttctatgata atattgagcg ctttccaaag acctaccatc
     1261 tcacatttga tagcggcttt aatcttacga taagcataag cattatcggg gatgagttgg
     1321 tcggaacatc agattatgga attgtaagcc cagttagaac tcttgccttg acaaatatca
     1381 cctttaatag gatcttaaat atatttttag atataccttg cggtaaaaaa tcagatataa
     1441 tcaaaattcc tctattactg atggactcac cccctactgt caagatagca gcgataaatg
     1501 cagtcttatg ctcagaatcc ggggtagagg ataaacgagt gagaatactc atgaacagcc
     1561 gacattatat tgatgattta agggtgctat ttatagactt aggtatccct gttctcgtta
     1621 gggagacctg cctatccata acgggtagga aaaatttgca gaaagtagct gagtcgctta
     1681 atttcatctc gactaagaaa caaaagaaaa tgaaaactaa tctgctgtct tacaaacgtg
     1741 atgttttcca aaaaggcgag gccttaaaag agattttgct tgctttgaag gaaaaagact
     1801 atacaacctt ggaattgtca caagagctta ataaacataa ggatacaatt caacatcatc
     1861 ttcaaggagg gattaaggaa aattttataa aaaaggattc aatctcttgg ccttacaaat
     1921 actcaattac ccctgaaggg tcagagtatc ttggtaggtg ctaaatgata agcttaggca
     1981 tcgagagtac tgcactctaa aggcagagat actttcggtg ttggaatagt agacgaaaac
     2041 ggaaacgtat tagccaatgt caaagatgcc tatacgacag aaaaaggtgg catccatccg
     2101 acagaggcaa agaaccatca tctaagagtt agggatgaag tagttatgaa agccttgcag
     2161 caggcaaact tatccttatc tgatatcgga ttgatctctt tttctcaggg tcctggcatg
     2221 gcaccttgcc ttgttgttgg catggaaaag gcaaaacaga ttgcccatga gttaaaaaaa
     2281 ccaataatcg gcgttaacca ctgcatctct caccttgaga ttggaagatt gttaaccaag
     2341 gcaaaggatc ctgtcatgct atatgctagc ggagcaaata ctcaaatcat cagttatgag
     2401 ggtggaaaat ataggtgcat gggtgagtca ttggacaatg gcgtcggcaa ctttcttgac
     2461 ggctttgcaa gataccttgg cctgggcttt ccaggcggcc caaagattta cgagcttgca
     2521 ttgaaaggaa aagaatatat cgagcttccc tatgttgtca aaggcatgga tgtttctttc
     2581 ggcggtcttc agacaaacct aaagcagaaa gttgactcgg gcaaatataa gattgaagat
     2641 ttatgctata gctgccagga aacagcattt gccatgctgg tcgaagtagc agaaagagcc
     2701 atggcacact gtgacaagca ggagctctta ttgggaggag gcgttgcctg caacgtcagg
     2761 cttcaggaga tgtgcaggat aatgtgcaag gaaagggatg cagaatgttt tatccctcca
     2821 aatagcgtgc ttgtggataa tggaggaatg attgcatggg aagggcttgt cgaatacaaa
     2881 agtcgtggtg cagacaaaat cgaatctctt gacatcaagc cctatgaaag gacagatgat
     2941 gtaaaagtca gctggcgctg agtttatttc ttgtaactgt gattgattgc attctcgaca
     3001 aaatttagac attaaaaatt tagagattgt tataacaaat gaaggccgca cgaaagctga
     3061 tatcaagctt agtgtatctg gcatcaacaa ataccttttc ctttcagaac aatcattaac
     3121 tctggaacca ggggagtcag cagaagtcga gctaaacttc gcaggaaaag atatcggagc
     3181 attcatcgga agccttattg tcgcagacca ggccatccct atccttctta tagtagaaga
     3241 agagaatgtt ctattcgatg tcagcatcga tctgaaaaac ccagggatac ctcaaggcaa
     3301 agatatccaa tcaagaataa ccctaaaaag catgggccag caaaaaaagg tcgatgtcta
     3361 tctaacttac atcatcaagg actctgaaga taatatcgtt ctaagcgagt atgagacctt
     3421 ggcagttgaa aaagagctgg tatatgataa ggcatttgac acaaaaaacc tccccaaagg
     3481 agattacatg gcttcagtcg aggctgaata tttagggcag aaagcaatat caggtgcttt
     3541 attctcagtt tttgctcagg agatgccttc cctgccgatc aaagctaact ataagccgat
     3601 tatcctactg ctgatagcag ctgcattgat actgttcaga aaaaaggcgg ttatcctatt
     3661 gttaaccatg ggatcaagaa gaaagacaaa aaaacccaga aaacattaag cctttgatat
     3721 cttgcttagt atattctctt ttcctttcca gactaatgct tcttttaaga catccttgat
     3781 atgctcaaca ggtattatct tgatcttgtt tagcttgtcg gcatcgatta ttatatcttt
     3841 taggtttgat ttaggaacaa tgactcgctg gatcccagct tcgattgcag cctcaacctt
     3901 tgaagagact cctcctatcg gtagtacatc tcctcgcaca gaaaggcttc ctgtcatcgc
     3961 agtatcctgc cttattggaa tttctttaaa tgcagagatg atggatgttg caacagcgat
     4021 agatgcagaa tcgccttcaa caccttcata tgtctgtaaa aactgcacat aaatatcata
     4081 tttctctttt aggtcttcac caaaatatct cttgacgatt gcagagacat ttttgatagc
     4141 ttcttttgcg atctcgccaa gctttcctgt cgcgattatc tctttttccc tgcctccagg
     4201 tgtaacctgg gattctatcg gtaagattat tcctgaaaat gactcatctt ttcccatgac
     4261 agcaaggccg tttaccctgc caaccttatc tccttcagtg atgatgacag catattcctt
     4321 tttcctttcg atatacctgt cagcaagctg cttttcaagt gtccttgcga ctgttttagc
     4381 ctctaatata tgctttttct caacaacctt tgctttcttc tcgcaggcaa ggtctcctgc
     4441 agctctgata aggccgccca gttcacgaag ccttagcgtc agatggcctt ttctgtttgc
     4501 catcttcttt gcttcctcaa tcatctcagt cactgcttcc ctgctgaaag cagggatctt
     4561 cttgtctttc tttatctcct gagcgacaaa tatagctatc ttatcacgat tctcttcatt
     4621 gtcaggcatg gtatctttca tgtatatctc atagccatat cctcttatcc ttgagcgaag
     4681 cgcagggtgc atgtccttga cagtctcaag gtttccagca gcaactaaaa taaagtcgca
     4741 aggcacagcc tctgtcctta ccatagcacc agcgcttctt tcagattggc cagtgatagc
     4801 aaacttcttt tcctgaagcg cagaaagcag ctcctgctgc gtatgaggct gaagcgttgc
     4861 catctcatcg atgaacaaga cacccatatg ggctttgtgt atcattcctg caactactct
     4921 ttcatgagca ggtgtcccaa gcccgcctga gtttttgcac aggatgccgt ttactattat
     4981 gttgcctgaa gaagttgtca agttatatag cttgcccttg taatctatct gtgtagtttt
     5041 gataactttg cttgttgtca gtttcatata gctgcctcag gatagttgtt taagataaga
     5101 tatagtgagt ttgggcttag attgagaacg tccattgctc gctcagcccc atagcctttt
     5161 tttgtgagtt cattaaattt atctcttttt agcgctgccc attgtccgag cgccaagtat
     5221 aatctgtctg tcttgtatct gcaatagttt aactttatac tcatcatgaa gaggataaca
     5281 ttatccatct ttttctctat caacatccga tagataagtg agtcatctct attcgtcctt
     5341 ccttcataaa tcgatgttac atttactctg ttccttttta aaaaatcaac gacttctcca
     5401 aggaaggaaa gtcgattgga tttaaggtca ggagagccgg ttatacctac ctccaaagac
     5461 gttaaatagt ttcctctttt gtgtattatc ggtgtcccaa gctcgcctcc aaggagagag
     5521 ccataaaatt ccgcctccaa ttcctgcctt aattttatcc attcaggtac tttcaatttt
     5581 aattttgttt tatttcccct aggtgcaccg agagctaaaa aaaggcggat gatgtttctg
     5641 ttggtaattt gacagcacca tgaatgtcct ttctcgcctc cttcaataat cctagagttt
     5701 ccgcttattt ggaacacccc ttctaaatcc tttctaaatt cactaacagc ttcaatctct
     5761 gaacttgaga gaaatattcc gttcaaatta tcaaaaatgc ctccatcgcc ataggtcgcc
     5821 ccaagtatct ttgcgattaa aggcagctta ggattatcaa ttttaagagg aagcagccct
     5881 ttcttcttta gccaatcggc tgtttggagc ggcacaggat aatgcttttt tgcatgccac
     5941 cgcctagttt tttcataagg ctgattcatt gctgcggcaa tcctcttata cccccagcct
     6001 ggatgcttag ccttaatatc aagatattga tgatacagcc tgcattgttc ctgttgacgc
     6061 aggtcataag tgttgatgat gtcttgttca tcaatgataa tgtcatttgc ccaaagttct
     6121 gttccttctt tgatatttct tgcctctgta tagcttattt ttccgttgtc caaaacagct
     6181 atctggtgtt ctggagtaac tgtcagttct ctgttctcgg aagtggtgag ctttatcatt
     6241 ttaccatcat aatcatatct gttgcatgat aaaacctccg ctgtagaggc acaaccctta
     6301 gtctcgccca agacgaagag ttcatttttt ggaagaaaaa cagcttcgta attgttccct
     6361 tttcttagga tgttgtcgct atgcttttcc atcagggagt caaccgtttg gtttatttta
     6421 tatggctgaa caccgttatt ggtaataatc tgtaacaact gttcagtaaa aaaagtttgg
     6481 aaagggtcat gaagcacgtc tccaagcagc gcgccagcat gtgttccggt tgcatcatta
     6541 aatggtgcta gctctttatt gaaattatca acaattacct taggtatcct tacttgagga
     6601 gcagccatcc tttttcctac atttaggaag atcaggaatg acgctaaaaa tagcatccct
     6661 ccaaggaaaa atgctgtaaa tatgatagcg ccagcagtct ggccaagctc tttggtataa
     6721 tggttaagtg cccaccatgg agcgatcatc gcaattatca ataatacaaa catcagtatg
     6781 ttctggttct taaaaagccc catagagctt atccttgact ttgctacaag ctccctgccc
     6841 tggcctccag gaacagtacg gatcaatgga gaattctcat cattagggtt tggaaaagaa
     6901 aggatatcaa caagcttttc ctttggaaga agctcagcaa gcccgagtcc aagcattgac
     6961 ttgccagtgc caggctcgcc aataagaagg acatgccttc tctgctgcgc tgctttcttc
     7021 attatattaa cagcagcttc ctggccgatg acctggtcga tgatgttttt aggtatcttt
     7081 atgtcagctg tcgtcttgaa ctttagcatt atattctaaa agatataagt atttttaaag
     7141 cttttgaatc tcagggctct ttgggggtcg aattgaagga caagacattg ctaaagattg
     7201 cgataacggg ttctcttgtg gggatagcgc ttcttatcct gctgtctcaa ttctaccagg
     7261 taggcgaaac atccataatt gacataaaag acgggcttat cggaagcgac gtcaaggtag
     7321 aaggcacagt caatagtata tttagaacca acagcacaag catatttacc ctctcctctc
     7381 catcagagct taagataatg gcaagcacaa atctgtccat ctcagaaggc gattatgtgg
     7441 aagtcatagg ccagatagat gaatataaag gagaaaaaga gattattgct gataggataa
     7501 gaaagatcga atgactttat tctacccact ttaccttcca ggttattatt ctttctccaa
     7561 gtacgcttct tgtatcataa aatctgtcaa actgctttgt tcctgcgcca tcaaagaaaa
     7621 ataaccttga aaacatgctt ccggcaagct ctggtgaaga aaatactgca taggcagcat
     7681 ccttatcttt gactacagtg atgcccaggc ctgcagcatc tccctcgctc tttataatct
     7741 caaactttcc attatcgaca agtcctatcc tggcaaggtt ttgttttgag ccgtctgatt
     7801 tttggatata tccttctttc tttgcaaagt ctatctttac aggaattgtc ccgccttgct
     7861 gcgaaaagcc gcattcagca atgtcttgag taagcgggca ctgcatcttg ccagttataa
     7921 accctggcca tggggctatc caggcattta tcgattcctg actgccaagc cctcgccgtt
     7981 catcgatata cttgcgggta atctgctctt caacatcata ttccttaaac ttatcgatta
     8041 tctcctgttc aaagcgagaa tcgacagcag aattgatata agacttcttg aagtcccagc
     8101 tgccaaagtg cgcccaaaca cctgcctttc ctatcatgtc ttgagaggtt atcaggaagt
     8161 tttcaggcgc tttgcagtgt gtgagaggta tgactttttc gatgtcattt tcagcaagct
     8221 tatgctcctt taggatttgc cttgcttctt tctcaggcac tgttattatc ttgttgagca
     8281 catctatcga gtgaggaata tcgcccagaa ctttgttgat ttcatcaaaa gcgttgtttc
     8341 caccgcagtc cagcatccta agtattccca ctgaggtgtt ttcatcagat gtcaaaagaa
     8401 gctttccaag ccagtggagc tggggattgt tctgtgaact cccgtcaaga gtcaccctgc
     8461 ggtcagcata gtatttgaac cagtgcccaa aatcccacca gctgttgatt attgcatcag
     8521 gctttgactc atctcttatc ttctcaaggc ttgccaccca ctcatcagtc acattgggta
     8581 tatactgctg tgcagcagat atcccggctt gcacaggagt cataaggaac cagatagcag
     8641 ctaaaatgga aaaggcttgg aatgcagctc catggatctt gaggtttttc ctgagtgtcg
     8701 acacaagaag tgaaaggcat ttgccaaaaa aaatagatac caatacatag acaggtatgg
     8761 ccatcaggaa caggaacctt atccctgtcg tagtaaaata ggtgacaagg catataagca
     8821 tcgtgagcat gaaaattgca tcagggtgat agtcgccctc ttcctttaga tttgcataaa
     8881 gccctatggc aagaggtata agggcgatga agattgtgag agggtttgaa gcattgacta
     8941 ttatataata tgctattaca tcaaaggcaa gcactcccag aaacaaaggc agcttgttct
     9001 ttatgttctt aaatgaaagc agagttatcc caaaaagaac catcaaaaac acaggtatcc
     9061 gtaagatgct tctaaagctg ggcactatat ctccgatgct catgttgttg aactcagcga
     9121 cagttgtaag aacatttggc caaaggttca ggttagatgc gtgtttaaac ctgagcacat
     9181 cgaattgtga gccaagcatt gaaaagaatg ccccaatgtc tcctgacagt agtgtataaa
     9241 atatgaatac actggcaaca aatattgaca ctgagaccac attctctttt atcggaaggc
     9301 cgccttcact ctttttcatc attttcttga tatagatgta agccatcttt actaccactg
     9361 ccaggatgaa aaggtcaaac agataccacc atccagacca aaacttggag tataatgcaa
     9421 ggcaagctcc tgcaagagct ccccaggtat acttcatctt tggatttgct gcataaaaga
     9481 actcaaatgc aagccaaagg aagatagcct ggaacaatat attcaatata tcactgtccg
     9541 agccaaggct tcttgtcaat acaaaaggat tgacatttac aagcgttgtg ccaacgaccg
     9601 ctccaatatt tcctgcgacc tttcttatcg caaggaaaag aggtatcgaa agcaaaagcg
     9661 acagcacaag gggggttaaa aatgaagcct gcatcaggct catatccttg ttaaaaattt
     9721 taaaaaactt gtatacccag actatgacga tggggtagaa atcgataggc ttctcatttt
     9781 ttaatggagc aacggtgtat gtgtcgtagc atttgccgtc ggtttcatca tcacactgtg
     9841 tccctttttc gatgatgttg cgtgacatcc tcagccaata ataagaatcg atatcgccga
     9901 gataaacata ggtgctgtct cctgaggtat attgcagcct gcttttgaaa ccctcagagt
     9961 ttgccttgat ctggtctgtg atgagctgct tgttctcgcc gacaaatttt gcatattctc
    10021 tgtcggcaag tttcaccctc tcctgtgcag gcaggtttgg aaattggttt gctatctgaa
    10081 ggcttatctg ttccttgaag tatgcattca cgcttgattg ggcagagccg tcaagcggtg
    10141 caagcttcat tggctcaagc ctgaccacag cagtgatgac taacgggata agtaaaagga
    10201 gtatccatga tttgcttaac agcttttctt taaggttttc catttcaaag gcgaataaat
    10261 cctattttta taaatctatt gctttggccc tcaggaaacc gttttaggag atgcagcttg
    10321 catagcaggc attctcttaa atagtaccgt ctgccgctag cttctgtact ataacatcta
    10381 aattttacga taataaaagt aaactataaa tataagtaat tcttaggcct tgtttggtga
    10441 tataatggag caaatactac ttaccccaaa agagcttaga cgttatgaaa tggtccaggc
    10501 acgt
//
LOCUS       DASRUK010000106         6352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1909365, whole genome shotgun sequence.
ACCESSION   DASRUK010000106 DASRUK010000000
VERSION     DASRUK010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6352
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1909365"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..578)
                     /locus_tag="VFF28_04595"
     CDS             complement(186..578)
                     /locus_tag="VFF28_04595"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HZX44934.1"
                     /translation="MSGNSLKKFRCVRCGQCCSHIRGMMSEEEKAFLTEFAYGKLPLV
                     QLFPIERMSFPLFDFEARRFKEWQKEVNIDAKIKPSRVIFDLNTNSTIVVTYYMDFDS
                     CPFLKDDSCLIYDKKRAFICRLFPFIVS"
     gene            complement(550..945)
                     /locus_tag="VFF28_04600"
     CDS             complement(550..945)
                     /locus_tag="VFF28_04600"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HZX44935.1"
                     /translation="MKRVKDKIAEIETFLDELKGFMPDDFKDYKSDTVIKAACERYVE
                     KIIEAAVDLSFLIIKQKNFRIPQDDIDAFNILLSERVIDKDLAARLRDAKGMRNIIAH
                     QYGEIDDKIVFYSLKEKLEKDVREFIKKI"
     gene            complement(942..1541)
                     /locus_tag="VFF28_04605"
     CDS             complement(942..1541)
                     /locus_tag="VFF28_04605"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZX44936.1"
                     /translation="MVIILTLKEFFRNNQNARKIFGKKEIDIIIRQLEGKNLTQSERN
                     RLSRDIRPKLQFIKDIGKFEGEFGLEKNQENNRLIEGTVSVILNDKLSEDIKAILLFG
                     SYADKTYTFRSDIDICVVFKKDLPLDESTRFRIRIAGQLPEKIDIQVFNILPQKIKRD
                     IARNHKVLYRAAAYDNIEFSIKYLKDEDYFIRMRRLMEA"
     gene            complement(1591..3051)
                     /locus_tag="VFF28_04610"
     CDS             complement(1591..3051)
                     /locus_tag="VFF28_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZX44937.1"
                     /translation="MRWIIIIGILLITIYSANAACQYASSAAASSQRLGSLASYATGA
                     PNAKTDGCDIWSGKGYSWSPYTWDRIARLNLRFPSAMKASELTVYGDYDMCWGRIWLK
                     NSKTGARKLIFDGEDNSCTYNKQLAGDFLADMVILETCGWSWSSTDAVRLCDNGGTPG
                     PVCGNRVIESGEQCDDGNTASGDGCSSTCQKEGSSTGAKICDWYNCAAGAASVSVDDS
                     FTTCRSQLNSHGWKGTFFLMETNQFTQSQWSTFRSIYNEGHEIGGHTQTHVCSGVTDN
                     EFRWEISSNRNDILENIGMPQSQLVTLAWPCGFAQKKDILSSYYKFARGYFYNQLEDK
                     NPSDFLELKSFNTPHYNQPSEEPPSYFSVADQAEAQGRWANLVFHNECSDSGAIDYLA
                     TKNLWVAPIGKVAKYIKERQSSSITNFNDRESSIGFDLSVSTLHGQALTVEANIGSRT
                     PSSVKVNGESKIFRKVGSNVRFSFVPRGNDHIDIVF"
     gene            3511..3978
                     /locus_tag="VFF28_04615"
     CDS             3511..3978
                     /locus_tag="VFF28_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44938.1"
                     /translation="MGEIYTIEDIIEIYKSAGMPYIVGNIIFPSDIPKRKFTPPSGNE
                     ALDVANSGGRYARGLGLIRNDSWHLGDDLEFDTYIELGVGECFFRFETFQRFNEVMQL
                     FKQIRQAPPYYLMGTRAGGQSLDFQAVSKNDFDNYRYQCTSQEFLSELYAEKK"
     gene            complement(4224..4706)
                     /locus_tag="VFF28_04620"
     CDS             complement(4224..4706)
                     /locus_tag="VFF28_04620"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZX44939.1"
                     /translation="MRSGAIENLELIEKQITLLEHKIEYGFEQMFNPLADVRGLNKIY
                     AAAIIAESEDIDYFKNRDQYYNFTGFVPRYAQSGKFESKFNHVNKCGSSYLRHYFFQA
                     AISLCRHNAYFKNLFIKKHVIEKKSKDEAHVYCAKKLCHIAFKLLKSKEKFCPEKLVH
                     "
     gene            complement(4648..5385)
                     /locus_tag="VFF28_04625"
     CDS             complement(4648..5385)
                     /locus_tag="VFF28_04625"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZX44940.1"
                     /translation="MKTVGIDVAKDEHKVCILDENKEKLRTFSFKNSHLGLLEFEKTI
                     SRYDTEFIIGFESSGIYHHGILYHLRNKYPNIAVLNPKRIKAHRKSLGYEYKTDKIDS
                     YIIADYLKERNLSNNTLPERYPLLKQLCRTRIKLIKQQTRLKNRITGDMHILFPEYER
                     CFDSIFCKASLSFLKNHIDPKVIASLSEKQIRDILVKSSKNVSYNHAKKILLAAKDSF
                     GIPKDGLEIEIRCYRESGADREADHSP"
     gene            5841..>6352
                     /locus_tag="VFF28_04630"
     CDS             5841..>6352
                     /locus_tag="VFF28_04630"
                     /inference="COORDINATES: protein motif:HMM:NF014177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HZX44941.1"
                     /translation="MLDFNPFPYQGSKRKIASSILKYFPKTEKLHLVEPFAGSSAISI
                     AAAYYNKTQSIWINDSNLSLIKLWNQIVFNPEGLSKQYSSLWKEQFANKKQFYNFIRD
                     EFNKTDEPHYFLFLLAKCIKGSIRYNANGQFNQSADNRRRGMIPGKMRSNLIMISQLL
                     RNQIKITSLD"
BASE COUNT         1798 a         1285 c         1174 g         2095 t
ORIGIN      
        1 gtgcctactt catatttctt ggataagaga tacatggtct gatatcttct tttttgttta
       61 accttgtttt ctttgttata cagtctctta aaaatacttg acgaatgatg aaatttggtt
      121 cttaatgcca tctatatcac atcttgctaa ttacagaagg ggtacttata taagaacttt
      181 tctatttaac ttactataaa tggaaaaagc ctgcagatga acgctctttt tttgtcatag
      241 ataaggcagc tatcatcttt caagaaaggg cagctgtcaa aatccatata gtaggtgaca
      301 actatggtgc tgtttgtgtt aaggtcgaaa ataactcggg aaggctttat ttttgcgtcg
      361 atgtttactt ccttttgcca ttccttaaaa cgccttgcct cgaaatcaaa caaagggaag
      421 gacatccttt ctatcggaaa aagctggaca agcggaagct ttccgtaggc aaactctgtt
      481 agaaaagcct tttcttcctc tgacatcatg ccccttatat ggctgcagca ctggccgcaa
      541 cggacgcatc taaatttttt taatgaattc cctgacatct ttttccaact tttcttttag
      601 cgaataaaaa actatcttat catctatctc accatattga tgagcgataa tgtttctcat
      661 gccctttgca tcccttaacc ttgctgcgag gtctttgtct ataactctct cgcttaggag
      721 tatattgaag gcatcaatgt catcctgagg tatcctaaaa tttttctgct ttataatgag
      781 aaacgagaga tctactgctg cctcaattat tttttcaaca tacctttcac aggctgcttt
      841 tataactgtg tctgacttat aatctttgaa atcatcaggc ataaaccctt taagttcatc
      901 caggaaggtt tcaatctctg ctatcttgtc ctttaccctt ttcatgcttc catcaacctt
      961 ctcatcctta taaaataatc ttcatccttt aagtacttta tcgaaaattc tatattatca
     1021 taggcggcag ccctgtataa aaccttatgg tttcttgcta tgtccctctt gattttttgc
     1081 ggaagaatgt tgaatacctg tatgtctatc ttttctggaa gctgtcctgc aatccttatc
     1141 ctaaatcttg tcgattcatc caagggcaga tcttttttaa acacaacaca gatatctata
     1201 tcagacctga aggtgtaggt cttatcagcg tatgagccaa acagaagtat agccttaata
     1261 tcttcactta gtttatcgtt caaaataacg ctaactgtgc cttcaatcaa tctattattt
     1321 tcctgatttt tttcaaggcc aaattcccct tcaaatttgc ctatgtcttt aatgaattgc
     1381 agtttgggcc ttatgtccct gcttagcctg tttctttcag actgcgtcag gttctttcct
     1441 tcgagctgcc ttataattat atctatctcc ttctttccaa aaatctttct tgcattctgg
     1501 ttattcctga aaaattcctt taaggtcaat ataatcacca tttaggatat attaaccatt
     1561 attaaggtta tctatttaaa tcttacgaaa ttaaaatact atgtcaatat ggtcgtttcc
     1621 tcttgggaca aagctgaacc ttacattgct tccaaccttt ctaaatattt tactttcacc
     1681 gttgactttt acagatgaag gtgtccttga gccgatgttt gcttctacag taagggcttg
     1741 gccatgcaaa gtagatactg aaagatcaaa gcctattgag ctttccctgt cattaaagtt
     1801 agttatgctt gaagactgcc tttctttgat atactttgca acctttccta tcggtgcaac
     1861 ccataggttc tttgtcgcaa gatagtcaat agctccgcta tccgagcatt cattgtggaa
     1921 gacaagattt gcccatctgc cttgggcttc tgcctgatct gcgacagaga aatagctggg
     1981 cggctcttcg cttggctgat tgtaatgagg agtgttaaag ctttttaact caaggaagtc
     2041 agagggattt ttatcctcaa gctgattgta gaaataaccc cttgcaaact tgtaatagct
     2101 agatagaata tccttctttt gggcaaaacc gcaaggccac gctaatgtta caagctggct
     2161 ttggggcatt ccgatgtttt ccaagatatc attcctgttg gaagatatct cccagcgaaa
     2221 ttcattatct gtcacgccgc tacagacatg tgtctgagta tggcctccta tctcatggcc
     2281 ctcgttgtat atcgacctga aggtgctcca ttgggattgg gtaaactggt ttgtttccat
     2341 aagaaaaaag gtccctttcc agccatgaga gttcaactgg ctcctgcaag tcgtgaaact
     2401 gtcatcgacc gagacagagg ctgctcctgc agcgcagtta taccaatcac agatcttggc
     2461 gcctgtagat gagccttcct tttggcatgt gctgctacat ccatcccctg aggcagtgtt
     2521 tccatcatca cactgctcac ctgattctat caccctgttt ccacataccg ggccaggggt
     2581 tcctccattg tcgcatagcc tgacagcatc tgtactgctc catgaccagc cgcaggtttc
     2641 caggatgacc atatcagcca gaaaatcacc tgcaagctgc ttgttgtagg tgcatgagtt
     2701 gtcttcgcca tcaaagatta atttgcgtgc acctgtcttg ctatttttaa gccatatcct
     2761 tccccagcac atatcataat caccatagac cgtaagttca gaagctttca tcgcgcttgg
     2821 aaaccttagg ttcaacctgg ctatcctgtc ccatgtatag ggcgaccagc tgtatccttt
     2881 acctgaccag atatcgcagc catctgtctt tgcattcggt gctcctgttg catacgatgc
     2941 aagcgagcca agacgctgcg aagatgcagc tgctgacgaa gcatattgac aggctgcatt
     3001 tgcagaatat atagttatca ataatattcc tatgattatt atccacctca tgatcattca
     3061 ccccatgaaa ttatgcagaa attaatcatt gactttaggc aatagccaat taaatagttt
     3121 tctttctcct ttagaatact aatcacacct taaggattat gcgataggaa tatagcagcg
     3181 ctgcttctct actcaaaagt ctttgagcct taagccttgc tctttagagc gactttggag
     3241 tataaagtta tggtctaaaa caaaggcttg ttatatgccc tgagccaagg cgaaagtgca
     3301 acggggcaag gtttaagttc tgacaaaagg gaagaacatt aaaccgcaga atcgaatttt
     3361 cactttttta ttagattact gctgaaggca gactgaatag aagtcggggg cgttctatca
     3421 acagagcgaa aagcgatgca taaagaacaa gaataaaggg gctttgattc ttaagataga
     3481 tttataatta tataatcatt ttttcataat atgggcgaga tatatactat cgaggatatt
     3541 attgaaatct ataaatcagc aggtatgccc tatattgtag gtaatattat ctttccttct
     3601 gatattccaa aaagaaaatt tacaccaccg tctggaaacg aggcattaga tgttgctaat
     3661 tcaggaggtc gatatgcaag aggtttaggt cttattagaa atgatagttg gcatctcgga
     3721 gatgatttag aatttgatac ttatatagaa ttaggagttg gagagtgttt ttttcggttt
     3781 gaaacttttc aaagatttaa tgaagttatg caactcttta aacaaattag acaggctcca
     3841 ccttactatt taatgggaac aagagcaggg ggacaatcgc ttgattttca ggcggttagt
     3901 aaaaatgatt ttgacaatta tcgatatcaa tgtacaagtc aagaattttt gagcgagtta
     3961 tatgctgaaa agaaataagc caaaggagcc ccattgtaat ccagcccccg acttcttaaa
     4021 aagtgaaaat tcgattgcgt atcgtatccc ttttcagcac aatatcagtt tatgaatatc
     4081 aagttataaa gaagcattaa gcttcctcat aacctttttc tcttcgggta attaagatat
     4141 aaatcatgct cctattttaa ccttgcgatt ttatcgtcta aaaaatgata tagctaacgc
     4201 tatggcatgc cctgaaatgg aggttaatgc acaagttttt cagggcaaaa cttctccttt
     4261 gatttcagga gtttgaaagc gatatggcac agcttctttg cacagtaaac atgcgcctca
     4321 tcctttgatt tcttttcgat aacatgcttc ttgataaaca ggttcttgaa ataggcatta
     4381 tgcctgcaca aggatattgc tgcctggaag aaataatgcc taagataaga agagccgcac
     4441 ttgttaacat ggttaaactt tgactcaaac tttcctgact gagcatatct ggggacaaac
     4501 ccggtaaagt tgtaatactg gtccctgttt ttaaagtagt caatatcctc tgattcagca
     4561 attattgcag ccgcatatat cttatttagg cctctaacat cagccagagg attaaacatc
     4621 tgctcaaaac catattctat tttatgctca aggagagtga tctgcttctc tatcagctcc
     4681 agattctcta tagcacctga tctcaatttc cagaccgtct tttgggattc caaaagagtc
     4741 cttggccgca agcaaaatct tctttgcgtg attgtaggat acatttttgc tcgacttgac
     4801 aagaatatct cttatctgtt tttcggaaag tgacgctata accttgggat caatatggtt
     4861 cttgagaaaa ctgagtgaag ccttgcagaa tatgctgtca aagcatctct catactctgg
     4921 aaaaagaata tgcatgtcgc ctgttatcct gttcttaagc cttgtctgct gcttgattag
     4981 tttaattctt gtcctgcaaa gctgctttag gagaggatat ctttctggaa gggtgttgtt
     5041 ggagaggttt ctttccttaa ggtagtccgc tatgatgtag gaatcgatct tgtccgtctt
     5101 gtactcatat cctaagcttt tcctgtgggc ctttatcctc tttgggttta ggacagcaat
     5161 atttggatat ttatttctta gatggtataa tattccatgg tggtatatgc ctgagctttc
     5221 aaagcctatg atgaattctg tatcatatct gctaatggtc ttttcaaact ccaaaagccc
     5281 taaatggctg tttttgaagc taaatgtcct tagcttttct ttattctcat caaggatgca
     5341 aaccttatgc tcatcctttg cgacatctat acctacagtc ttcattctaa tcaaacctcc
     5401 gaatgctttt tatacgctaa aggtttacat gttttcattt gcgtagtatc atggtacaca
     5461 gtcagtgctg tagtcaactc catccacgct tgaatgaaaa accctgttgt ttaacaatca
     5521 cgctcgataa cctagggtta tggcgtagta cgcaatgcac agggtaaacc tttatgtgca
     5581 taagggataa agaagctggc tattagcaac taattttcct accttttaac agtagtttaa
     5641 ttgattatag ttgtttaaca aagccgcggc tcgttaaatc ctttttccat tatgcagtgt
     5701 ggaaaaactg cgtccgcaga gtaattttta gtggctttct atccagcgaa caaagtgagc
     5761 aatcaaaaat taatcgttaa gggggacatt tctaaacgtt tctaataaaa tttatatagg
     5821 taagtgttta agtcgtagat atgttagatt ttaatccatt cccttatcaa gggagcaaga
     5881 ggaaaattgc atcttcaatt ttgaagtatt tccctaaaac tgagaaattg catttggtag
     5941 agccattcgc tggttcgtca gcaatatcta ttgcggcagc ttattacaat aaaacccaaa
     6001 gtatttggat aaatgattcc aatttgtcat tgataaaact ctggaatcaa attgttttca
     6061 atcctgaggg gctttctaag caatattcct ctctatggaa ggagcaattt gctaataaaa
     6121 agcaatttta taattttatt agggatgaat tcaacaaaac agatgagccg cattacttcc
     6181 tttttttact ggctaaatgt atcaaaggtt caataagata taatgctaat ggtcagttta
     6241 atcaaagtgc ggacaataga agaagaggga tgatacctgg aaagatgagg tctaacctga
     6301 taatgatttc tcaactgttg agaaaccaaa tcaagataac ctctcttgat ta
//
LOCUS       DASRUK010000107         5279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_418813, whole genome shotgun sequence.
ACCESSION   DASRUK010000107 DASRUK010000000
VERSION     DASRUK010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418813"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..157
                     /locus_tag="VFF28_04635"
     CDS             <1..157
                     /locus_tag="VFF28_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013998908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44942.1"
                     /translation="AKKEEEIYFPGLSIPIKIKGKGLRLLQEIRDEAHRFAIKYNRLL
                     RIKAMKE"
     gene            complement(170..1096)
                     /locus_tag="VFF28_04640"
     CDS             complement(170..1096)
                     /locus_tag="VFF28_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HZX44943.1"
                     /translation="MYIEKRRLGNSIKYYLVHSYREKGQVKKLRRFLGRNLSPGQLDS
                     IKESVSVQIRRELELFNTEVFEFSLTPKQVQRLNKMDKIMEICHLEEFDWKLFAEQFT
                     YHTNAIEGSTVKLDEVPKILHKEKVSDEEEIETQGVAASIDYVKRTKEELSLKLILNI
                     HNICFKGSKHFAGKFRDVEVVIRNARGEIIHRGVPASEVKDHLEELAKWYKINKKRFK
                     PLVLAALIHNQFEDIHPFQDGNGRGGRLLLNYILIKNNYPPINISLEDRQIYYSSLQE
                     YHKSHDIKPTLQFLVNQYKKTLKQVPTKCTKK"
     gene            1358..2221
                     /locus_tag="VFF28_04645"
     CDS             1358..2221
                     /locus_tag="VFF28_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44944.1"
                     /translation="MASLLSLWQNGQIDKASGNLDDAVRWIEEHGSNVQVPDRINLDL
                     IRQPFDGSIHLFPHHCDISRATITAPLDVVISSHGDTTGPLKGLKPNGHRGPSMRFFD
                     VNAREILTLEALPWAYIPDGDFKAMARMDAFSATLHYENGGDISEAGGISWLELPIGN
                     VPDPKKCESKIRKIKAAGGVLLRDYGHREVQWDGTNYHIYPTTEKVTSILLFGKGKAA
                     VHLTRQPDPKGNYITYSIPPSYWTVPISIIFDLFSEKYDLSAINFRTGFNLMRPSLYE
                     TLRKGGSVKGY"
     gene            2263..4194
                     /gene="uvrB"
                     /locus_tag="VFF28_04650"
     CDS             2263..4194
                     /gene="uvrB"
                     /locus_tag="VFF28_04650"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HZX44945.1"
                     /translation="MDFKLKSDFAPKGSQPEAIKKLVAGLDKGNKYQTLLGVTGSGKT
                     FTIANVINQHQKPTLVFAHNKTLAAQLYNEFKEFFPENRVEYFVSYYDYYQPESYIPS
                     KDQYIEKDSAVNPKLEQLRLSATASLASRKDVIVVASVSCIYNIGDPEEFKGMGFDIS
                     VGMKVDRQDILKKLVDIQFERSDIELPPGSFRVKGDTIDFVPGYFNNIIRVEMFGDEI
                     EKISEIDKVTGNQKESMKYFFIYPAKHFVIPEDNQKQALAEIEHDLNQRLPELGMMEA
                     YRLKQRTMYDVEMIREVGMCKGIENYSRYFDGRKPGEKPFCLLDYFKDFLIVIDESHQ
                     TIPQIHGMYNGDLSRKKTLVEYGFRLPSCLDNRPLKYNEFEKYMEDNHVIFTSATPGD
                     YELSKSKQVVEQIIRPTGLVDPIVEVRPIKGQIEDLKKEIERTIANKDRVLVTTLTKK
                     LAEELTEYLASQKIKARYLHSEIDTLERTEIIRELRLGKFDVLVGINLLREGLDIPEV
                     GFIGILDADKEGFLRDFKSLIQIIGRAARNVNSKVVLYADKMTDSMKNALSETERRRK
                     MQMEYNAKNGITPVTVIKPIRDKEVDIKDTKHIPKQDIPNLVIELEIEMRQAADSLDF
                     ERAIALREQVKRLKDRIRQ"
     regulatory      4210..4333
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4366..>5279
                     /locus_tag="VFF28_04655"
     CDS             4366..>5279
                     /locus_tag="VFF28_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44946.1"
                     /translation="MLAEAAVRVFWQNPFTPLFQSGIGFQRQSKAEAQETDEQTIYFE
                     AKPNTTLDMLVNNLLLSNAGRLETMAVPDYSPYPQFSQALESPVYILVASLWGRTYSS
                     IVYDTHHTTDFSEMYQTSTPYYHAEMPNYEDVVPYEDNPEHLTAKSVYAYQDAARHYE
                     TTYKYSVSQRISHSSAGINLEYVMPNVKIWLPNIESKADIPYEKIMPIDGEKSNTVYN
                     GQPGVQVLDLDSRVLNKNKPAISEALPQKPSTARFAFQCGVFDMSEIASGARSDVSEI
                     AYGTYFVHALPMTHQAISEYAQKLITNR"
BASE COUNT         1634 a         1009 c         1169 g         1467 t
ORIGIN      
        1 tgcaaaaaaa gaagaggaga tatattttcc tgggctttct atccctatta aaataaaggg
       61 aaagggacta aggcttctgc aggagatcag agacgaagcg cataggtttg cgataaagta
      121 caacaggctg cttaggataa aggcaatgaa ggaatgaggt gaccacagcc tatttttttg
      181 tgcattttgt gggcacctgt tttaaggtct ttttgtattg gttcaccaag aattggaggg
      241 ttggcttgat gtcatggctc ttgtggtatt cctgaagcga cgagtagtag atttgcctgt
      301 cctcaaggga tatatttatc ggcggataat tgttttttat caagatatag ttcaaaagaa
      361 gcctaccccc cctgccattg ccgtcttgga agggatgaat gtcctcaaat tgattatgga
      421 ttagcgcagc taaaaccagt ggtttgaatc tcttcttatt aattttatac catttggcaa
      481 gttcttccag atgatctttc acctcactag caggaacccc cctatggatg atttcacctc
      541 ttgcattcct tatgacaact tccacatccc taaacttgcc ggcaaaatgc tttgagcctt
      601 taaagcagat attatgaata ttaagaatta gcttcaatga aagctcttcc tttgtcctct
      661 tgacatagtc aatggaagct gcaacccctt gcgtctcaat ttcttcctca tcacttactt
      721 tttccttgtg aagtatcttt ggcacctcat caagcttgac cgttgagcct tctattgcat
      781 tggtatgata tgtgaattgc tctgcaaaca atttccaatc aaattcttca agatggcaaa
      841 tctccattat cttgtccatc ttgtttagcc tctggacttg tttaggagtt aggctaaatt
      901 caaatacctc ggtgttgaat agctccagtt ctcgccttat ctgtacagaa acagactcct
      961 ttatgctatc cagctgtcca gggctaaggt ttcttccaag gaatcttcta agctttttta
     1021 cttgaccttt ttctctataa gaatgcacta gatagtattt tatactgttg ccaagcctcc
     1081 ttttttcaat atacatatat actaatcagg tgcccacatt tataataatt tcgtttttgt
     1141 ggtcacccaa tattgaggta aatggtataa actagaaaca aactccaaaa acatatagct
     1201 gagtacttga tatacggcca gtgctttgtg tgcttcataa tcacctcttt agaaaacaaa
     1261 ggataagtga tttctgacta tttaaaggta tttttggatg tcaagcgttg actagccaag
     1321 catgagcttt taaagataaa aataatctag ttaagatatg gcttctctgc tatctttatg
     1381 gcaaaatgga caaatagata aggcatcggg taatttagat gatgcagtaa ggtggattga
     1441 ggagcatgga agcaacgtac aggttcctga taggattaat cttgacttaa taagacagcc
     1501 ttttgacgga tctatccatc tgtttcctca ccattgtgac atcagcagag cgacgataac
     1561 tgcccctttg gatgtagtca tcagttctca tggggatacc acgggaccgc taaaaggtct
     1621 gaaacctaat ggacatagag gaccaagcat gaggttcttt gatgtaaatg ctagggagat
     1681 ccttacgctc gaggcattgc cctgggctta tataccggat ggtgatttta aggctatggc
     1741 aaggatggac gcatttagtg caacattaca ttatgaaaat ggcggagata taagtgaagc
     1801 agggggtata agctggctag aactgcctat cggaaacgta cctgatccta agaaatgtga
     1861 aagcaagata agaaaaatca aagcagcagg aggcgttttg ttaagggatt atgggcatag
     1921 ggaggtacaa tgggatggaa cgaattatca catataccct acaacagaga aggtcacatc
     1981 catactttta tttggcaagg gtaaggcagc tgtacatctc acccgacaac cagaccctaa
     2041 aggtaactat atcacttatt ccatacctcc atcttactgg acagtaccta taagcataat
     2101 atttgatttg ttcagcgaga aatatgatct tagtgcaatc aattttagga ctggctttaa
     2161 cttgatgcgt ccatcacttt atgagacttt aagaaaggga ggcagcgtga aaggttattg
     2221 agtaacaata tgcttaaata tctttaccaa taacccaatc gcatggattt caagctaaag
     2281 tcggatttcg ctccaaaagg ttcccaacct gaggctatca agaaactggt agctggtcta
     2341 gataaaggga ataaatatca gactcttctt ggagtcaccg gttctggaaa aacttttacc
     2401 atagctaatg tgataaacca acatcaaaag cctactcttg tatttgcgca taacaagacg
     2461 cttgctgctc aattgtataa cgaattcaag gagttctttc ctgaaaacag ggttgaatac
     2521 tttgtctctt actatgacta ttatcagcct gaatcctata taccttccaa ggaccaatac
     2581 atagagaagg attctgctgt caatcctaaa cttgagcagc ttcgcttgtc tgcaaccgct
     2641 tcccttgcat cgcgtaaaga tgtgatagtc gtcgcttctg tgtcctgcat atacaacata
     2701 ggcgaccctg aagaattcaa ggggatggga tttgatatat cagtcggcat gaaggtagac
     2761 cggcaagata tattaaagaa acttgtggac atacagtttg aacgaagcga tatagagctt
     2821 cctccgggca gttttagggt gaaaggagac acgattgact ttgttccagg ctattttaac
     2881 aatatcatca gggtagagat gtttggcgac gagatagaga agatatcaga gatagacaag
     2941 gtgactggaa accagaagga aagcatgaaa tatttcttca tctatccagc aaagcatttt
     3001 gtcatacccg aggataatca gaagcaggct cttgctgaaa tagagcatga cttgaaccaa
     3061 agacttcctg aacttggaat gatggaagcc tatcgcctga agcagagaac gatgtatgat
     3121 gtcgagatga taagagaagt cgggatgtgc aagggcatag agaactattc aaggtacttt
     3181 gacggaagga agccagggga aaaaccattt tgccttcttg attatttcaa ggattttctt
     3241 atcgtcatag atgaatccca ccaaacaata cctcagatac atgggatgta taacggtgac
     3301 ttgtcgcgca agaaaacgct tgtcgagtat ggatttaggc ttccaagctg ccttgacaac
     3361 cgccctttga aatataacga gtttgaaaag tatatggaag ataaccatgt tatctttacg
     3421 tctgccacgc ctggagatta tgagctgtcg aaatcaaaac aggttgttga gcagataata
     3481 aggccgacag ggcttgtcga tcctatcgtt gaagtcaggc cgataaaagg gcagatagag
     3541 gatcttaaga aggagatcga aaggacgatt gcaaacaaag acagggttct tgtcacaaca
     3601 cttaccaaga aactggctga agaattaacc gaatatcttg cctctcagaa gataaaagca
     3661 aggtacctgc attcagagat cgatacactt gaaaggactg agatcatcag agaacttagg
     3721 cttggcaagt ttgacgtgct tgtcgggata aatctccttc gtgagggatt agatatccct
     3781 gaagtggggt tcatcggaat acttgacgct gacaaggaag gttttttacg cgattttaag
     3841 agcctgatcc agataatagg aagggctgca aggaatgtca acagcaaggt agtgctttat
     3901 gctgacaaga tgactgattc catgaaaaat gccctgtctg agacagaaag aagaaggaag
     3961 atgcagatgg aatataatgc aaaaaatggc ataactcctg taactgtgat aaagccgata
     4021 agggataaag aagttgacat aaaggacaca aagcatattc caaagcagga catcccaaat
     4081 ctggtcatcg aacttgagat agagatgagg caggctgctg attctcttga ttttgaaagg
     4141 gcgatcgcac ttagagagca agtgaagaga cttaaggata ggattagaca ataagttacc
     4201 gacgacaagc ataagatatt tatataaggt gtttctaaaa taaaatattc aggataagga
     4261 aagtgctgtg aaaaccagcc gctgacccgc aaccgtaaaa gccggaatac ttgcttgaaa
     4321 ataaaggatc gtggagttgt ccgctggact tctctggagg gtaagatgtt ggcagaggct
     4381 gctgtaagag tgttttggca aaatccattt acaccgcttt ttcagagcgg gataggcttt
     4441 caaagacaga gtaaggcaga agctcaagaa acagatgagc agacaatcta ttttgaagca
     4501 aagccaaata caacgcttga catgctggta aataatctgc tgctttctaa tgcagggaga
     4561 ttagaaacta tggctgtccc tgattatagc ccttatccac aattttcaca ggctttagaa
     4621 agtcctgtat atatactggt tgccagcctt tggggaagga catattcttc tattgtttat
     4681 gatacgcacc acacaactga tttctcagag atgtaccaga catctacacc ttattaccat
     4741 gctgaaatgc caaattatga ggatgttgtg ccttatgagg ataacccgga gcatctgaca
     4801 gctaaatcgg tttatgctta tcaagatgca gctagacatt atgagaccac atacaagtat
     4861 agtgtgagtc agcgtattag ccacagttcg gctggcatta accttgaata tgtgatgccg
     4921 aatgttaaaa tttggctgcc aaacattgag agtaaagctg atatacccta tgaaaaaatc
     4981 atgccgatcg acggtgaaaa gagcaataca gtatataatg ggcagcccgg agtacaagtg
     5041 ttggatcttg actctagggt tttgaacaag aacaagccag cgatttctga agcattgcct
     5101 caaaagccct cgacagcacg ctttgcattt caatgtgggg tttttgacat gtcagaaatc
     5161 gcttcgggcg cacgctctga tgtgtcagaa atcgcctatg gcacatactt tgttcatgct
     5221 ttgcctatga cacatcaagc gatttctgag tatgctcaaa aactcattac aaacaggca
//
LOCUS       DASRUK010000108         8953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1432222, whole genome shotgun sequence.
ACCESSION   DASRUK010000108 DASRUK010000000
VERSION     DASRUK010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 8953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8953
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432222"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..600)
                     /locus_tag="VFF28_04660"
     CDS             complement(94..600)
                     /locus_tag="VFF28_04660"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HZX44947.1"
                     /translation="MQIALKTPLSEILRNGQSCDQCGHCCSHGSGFLVEDDIKHISSF
                     LRISEQELKEKFLEEKELFNTKRLRPKLDSKKPYGPCIFLKDNRCSIHEVKPLQCRIG
                     TCKGSGEEQGIWFSLNYLVNKDDPESVRQYSLYLKSGGKTIPGGRLEELVPDKEKLDK
                     MLNFQMLR"
     gene            complement(569..1099)
                     /gene="grpE"
                     /locus_tag="VFF28_04665"
     CDS             complement(569..1099)
                     /gene="grpE"
                     /locus_tag="VFF28_04665"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HZX44948.1"
                     /translation="MKEKKQEPEKNTEQEKMAPAQEKKEQSAEKIAELTDTLQRLQAE
                     FENYKKYVEKEKEMYRRYAKADIIEKLLPILDSFEFALKNQCDMESFRKGVELIYAQF
                     YGALEKEGLKKIEAKGKLDPNFHEVLLKAPSDKEEDTIIAELQKGYLLNERVLRHTKV
                     KVSKGKDANCAKNSAK"
     gene            1506..1910
                     /locus_tag="VFF28_04670"
     CDS             1506..1910
                     /locus_tag="VFF28_04670"
                     /inference="COORDINATES: protein motif:HMM:NF019884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HZX44949.1"
                     /translation="MEEIYKITIISLRRPLEKTINQELQWFGNSLGLFNLRDKDKSCF
                     RVFIELLKSAKRKQPLSSDELAIRLSLSRGTVIHHINRLMGAGIVIHSRKKYLLRVDN
                     LRELVEHIEKDLQKTCTELKETAKEIDRRLSL"
     gene            1907..3241
                     /locus_tag="VFF28_04675"
     CDS             1907..3241
                     /locus_tag="VFF28_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44950.1"
                     /translation="MKVAPIFLIILILPIATASIEITKEPVDGFLGDALVMSARVTAS
                     ESKAALAKFTINCGETETLYSVAPVELKSGEVTIVEASALKAFLPETCSITLKVEGLD
                     GSAIENTASKEFIITDILDFSVSAERKEFLPGEVIRVKGELSKKGRFADDSTIKINFD
                     EESRVIYPTTHQFEYNLKARSNIKTGTHTINLVASDEYGNQNSADIEINIKAVPSAFL
                     IFLGANEYKPNDNLAITSEIHDQADDLMAGKSYSKLYKKGSLLAKEIILFEASYEGKY
                     DYKLSNKIAPDDYLLRSTYANLSDERTVKIIPYADITITQSGSIIAIKNTGNVRYKNE
                     TSIMLAKDGKNYIINKKLNLGIDDEMKIDLEKEVPAGIYGLSVDGKAGNVEIKQGKKT
                     GIAAVTGFAVAGAGLIYRSPKLGLSLIVLALMGLICFYNRETIKRIINKFKN"
     gene            complement(3238..3747)
                     /locus_tag="VFF28_04680"
     CDS             complement(3238..3747)
                     /locus_tag="VFF28_04680"
                     /inference="COORDINATES: protein motif:HMM:NF003269.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15e"
                     /protein_id="HZX44951.1"
                     /translation="MSMYKYIREAWKNPKENLGDLWQKRIVEWKSEPATIRIDNPTRL
                     DRARSLGYKAKQGYLVVRQRVMRHKRQRPKFAGGRKSRHMRRKKVLNMSYQWVAETRA
                     EKAYVNCTVLNSYPVGGDGQHLWYEVILVDRSHPVIKADPHISWISNERGRAQRGLTS
                     AARKSQIKS"
     gene            complement(3869..4327)
                     /locus_tag="VFF28_04685"
     CDS             complement(3869..4327)
                     /locus_tag="VFF28_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44952.1"
                     /translation="MEAEEKRILEKWDLLSEQTKSMLKIIGVYPKWRKMAKELGIKVE
                     SLRLDRYYSAEAYVELCQEYLGKNTKFYLIPKSNIAHLGVGEWCSTVYRFLENIKAYL
                     EEYFQRNKSESGFSEDKKRTGWRFAQRREDRVETAYGITAAWHNLFKLGD"
     gene            complement(4335..4730)
                     /locus_tag="VFF28_04690"
     CDS             complement(4335..4730)
                     /locus_tag="VFF28_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44953.1"
                     /translation="MEQAHQGANDVKRMNEQVLILIDKERSRQFTKFIELFKEKPEIP
                     MTEVRSKIQKNAYYNFVLVKLDAINSTIRSLKPEMQFFISPAFSNRKGKIVHTLKKNC
                     SIEIIDNDPNFFYLKIKQGEQSTVRVNCT"
     gene            complement(4873..5079)
                     /locus_tag="VFF28_04695"
     CDS             complement(4873..5079)
                     /locus_tag="VFF28_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44954.1"
                     /translation="MEKTELLRYKDKRVQAKFRDGSLIKTAEGILKEVGDGFAKITGR
                     KGTIILNQGSIIKIKEETDSQYTL"
     gene            complement(5143..5484)
                     /locus_tag="VFF28_04700"
     CDS             complement(5143..5484)
                     /locus_tag="VFF28_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44955.1"
                     /translation="MIDKTLINTLSKLAKGEKKIKGFRNRARVKGQIVNKGKTKNGNI
                     RLKLKKGEDIYNFVVIKSHKERFGLAQKLKVGDLVSAQGVSRFRAVICTQLKQIKNID
                     ESKQVQLEGFS"
     gene            complement(5711..6325)
                     /locus_tag="VFF28_04705"
     CDS             complement(5711..6325)
                     /locus_tag="VFF28_04705"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44956.1"
                     /translation="MERYHLAEGKGYVEVDAKKTPRNLVFYGKTYSLSPAYSPPKAVK
                     MGEKRLKGWLRDQGISPIDITAAFKKPEQEEEGLTASVLDPIGRLGKKLTDVQAAQAH
                     AYRYRTPYDDLSSYQPSSRFIQETAGLVNGLQPHHNCLPLGFTKKNELAVAISDPGDM
                     DLTDTINTSTSYKILFVASPADKIEKALKQFYPAREPPRFDEFM"
     gene            complement(6481..6738)
                     /locus_tag="VFF28_04710"
     CDS             complement(6481..6738)
                     /locus_tag="VFF28_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44957.1"
                     /translation="MAGKTRDGIKLSTIVDMVMELGIAVREGTKHPYILQAPGLRPCP
                     VAESTDARSMLAPWIGSAVGYRARDVYESLRQGEWYYRAVA"
     gene            complement(6740..6910)
                     /locus_tag="VFF28_04715"
     CDS             complement(6740..6910)
                     /locus_tag="VFF28_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44958.1"
                     /translation="MISPPSTLQVERETRVLIKQYCFIHKIQVKQFVEQMANERLAEF
                     KKRLEELKKLNI"
     gene            complement(6921..7475)
                     /gene="purN"
                     /locus_tag="VFF28_04720"
     CDS             complement(6921..7475)
                     /gene="purN"
                     /locus_tag="VFF28_04720"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HZX44959.1"
                     /translation="MCRTAIFSTGDSRLVSAILGKSYPNIKVHCIVTNKPDSRVIDIA
                     KQHNLKWACFDQNNYSNRIDHEQAVLSFLKVENVQFCIFAHYMRLITKYFVDQYSNRI
                     INIHPSLLPAFKGAKGYQDAFNAGVNTSGCTIHYVDEGLDTGDIILQRSVPRLQSDTF
                     EIFRNRVHQMECEAIQTVLHAVAV"
     gene            complement(7487..7756)
                     /locus_tag="VFF28_04725"
     CDS             complement(7487..7756)
                     /locus_tag="VFF28_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44960.1"
                     /translation="MDGSCVLEPLRVEKPLETRLAETRQEQGSVRDLLAELWQVARQY
                     VREIGLKDTSLEYRTETGAIVLRDAPFVGTVPICAITANQIVPYF"
     gene            complement(7943..8683)
                     /locus_tag="VFF28_04730"
     CDS             complement(7943..8683)
                     /locus_tag="VFF28_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44961.1"
                     /translation="MDAVVSELGGSKEKVNLHYMCDPNAFAPANRDHTSECMEIIEAG
                     LGHTPWRIVFYDSALFVEPGKVIEDTSKWGIRQYGIGRETATAHAASFLGEKCCGVVK
                     SQDMLDAERKGIEDVVKRVKNSNMKFVFEISYIISPNDTAETLRKTFEEMDYFASMAD
                     SKTIMAIKFAMLSPLPGTRILTQHTDLIHEDTYAASGSYERWDERAIKTAFPNSVYPQ
                     LKQSRLFNDYSDTWNYRSNFLEAVRKLK"
BASE COUNT         2427 a         1969 c         1671 g         2886 t
ORIGIN      
        1 ttcttttttc gagcaaaatc tttccatctt tttcgatgac agctccggct gttgccctca
       61 cctttttccc tcttatcgcg gattggacgt ctatcatctt agcatctgga aatttagcat
      121 cttgtccagt ttttctttgt ctggcacaag ctcttccagt cttcctccag gtattgtttt
      181 tcctcctgac ttcaggtata atgaatactg cctgactgac tcaggatcat ctttgtttac
      241 gaggtaattt aggctgaacc atatgccctg ctcttctcca ctccctttgc atgtgcctat
      301 cctgcactgc agaggcttta cctcatgtat gctgcatcta ttatctttta aaaagatgca
      361 gggaccgtat ggctttttgc tgtcaagctt tggcctgagc ctttttgtgt tgaacagctc
      421 tttttcctcg aggaattttt cctttaattc ctgctcgctt atcctcagga agcttgaaat
      481 gtgtttgatg tcatcttcga caaggaatcc tgagccatga ctgcagcaat ggccgcactg
      541 gtcacatgat tgtccgttcc taagtatctc acttagcgga gtttttagcg caatttgcat
      601 cttttccctt gctgactttt acctttgtgt ggcgaaggac tctttcattt agcaggtagc
      661 ctttttgaag ctctgctatt atagtatcct cttctttgtc tgagggagct ttcaataaaa
      721 cctcatggaa gtttggatca agctttcctt ttgcctctat cttctttagc ccttcttttt
      781 caagggctcc ataaaattgt gcgtagatga gctcgacgcc tttgcggaag ctttccatgt
      841 cgcattggtt tttcagcgca aattcaaatg agtccaggat gggcaaaagc ttctcaataa
      901 tatccgcctt tgcatatctt ctgtacattt ctttttcttt ttcaacatat tttttgtagt
      961 tttcaaactc cgcctgaagg cgctgcaaag tgtctgtaag ctctgctatc ttttctgctg
     1021 attgctcttt tttttcctga gctggggcca ttttctcctg ctctgtgttt ttttccggct
     1081 cttgtttttt ttctttcata aacaattcac tttgcggaat tcattttttt tagtcatgtg
     1141 tcaaaaacca ccagttaccg ctcctttcag tcgctataga ctggtggcat gacggttaag
     1201 tggtttttga cgcttgaaac actaaaaaat ccataggatt tttggtgcac caaaaatttg
     1261 caatggcaaa ttttttagta ttctaccaga tagtggtttt tgagcacaat tcactccacc
     1321 agtcaaatag actggtggaa tttcattgtt taaatctgca tccacttctt tgttgattta
     1381 acatcaactt agtttaagtt aaataaacaa ctatttaaag atttctatag tcaagtatat
     1441 agattgatgg ataaagagat atttcaagca caagatttat atatccagcg gattattttt
     1501 ccgtcatgga ggagatatat aagattacga ttatttctct aagaaggcct ctggaaaaga
     1561 ctatcaacca ggaacttcaa tggtttggaa attcccttgg gctttttaat ctcagggaca
     1621 aggataagtc gtgctttcgc gtctttatcg aactcttaaa gtctgcaaaa agaaaacaac
     1681 ctttgtcctc tgatgagctt gccatccggc taagcctttc aaggggaaca gtgatccacc
     1741 acatcaacag gctgatgggc gcaggcatag tcatccatag cagaaagaaa tatttattaa
     1801 gggttgataa tctgagagag ctggtcgagc acatagaaaa agacctgcag aagacatgca
     1861 ccgaactaaa agagacagca aaggagatcg acaggaggct aagcttatga aagttgcacc
     1921 catttttcta atcatcctta tattaccaat cgccactgct tcaatcgaga ttaccaaaga
     1981 gccagttgac ggttttctcg gcgatgcact tgtgatgtct gcaagggtca ctgcctctga
     2041 aagcaaggct gcgcttgcaa agttcaccat caattgcggc gagactgaaa cactatattc
     2101 tgtcgcacct gtcgagctta agagcggcga ggtgacaatt gttgaagcct ctgcattgaa
     2161 agctttttta cctgaaacct gcagcataac cctaaaggtc gaagggcttg acggctcagc
     2221 catagaaaat acagcatcaa aggagtttat aattacggat atactcgatt tctcagtttc
     2281 agcggaaaga aaagaattcc ttcctggaga agtgataagg gtaaagggag aactcagcaa
     2341 aaaaggaaga tttgcagatg attctacaat caagataaac tttgacgagg agtcaagggt
     2401 gatatacccg acaactcacc agtttgaata caaccttaag gcacgaagca acataaaaac
     2461 aggaacacat acaataaatc ttgtcgcatc tgatgaatac ggcaaccaaa acagcgcgga
     2521 tatagagata aacataaaag ctgtgccaag cgcttttctg atctttttgg gtgcaaatga
     2581 gtacaagccg aatgataacc tggccataac aagcgaaata catgatcagg ctgatgacct
     2641 catggctggc aagtcctatt cgaagctgta taaaaaagga agcctgcttg caaaagagat
     2701 aatccttttt gaggcgagct atgagggaaa atatgactat aagttgagca acaagatagc
     2761 gcctgatgat tatctgctca ggagcacata tgccaacctt tctgatgaga gaacagtgaa
     2821 gataatacct tatgctgaca tcacaataac ccagtctggg agcatcatag cgataaagaa
     2881 tacaggcaat gtcaggtata agaacgagac aagcatcatg cttgccaaag acggaaagaa
     2941 ctatatcatc aacaaaaagc tgaacctcgg tatcgatgat gagatgaaga tagacctgga
     3001 aaaagaagtg cctgcaggca tatatgggct cagcgttgac ggaaaagcag gaaatgtgga
     3061 gataaagcaa gggaagaaaa cagggattgc tgctgtcaca gggtttgctg ttgcgggcgc
     3121 aggacttatc tatcgcagtc caaaactcgg gctttcatta atagtgcttg cactgatggg
     3181 attgatctgc ttctataaca gagaaacgat aaaaagaata ataaataaat ttaaaaatta
     3241 actttttatc tgagatttcc ttgctgctga ggtaaggcct ctttgtgccc tgcctctctc
     3301 gtttgaaatc caagatatgt gagggtctgc ttttatcact ggatgagacc tgtctacaag
     3361 tataacttca taccatagat gctggccgtc gccacccact ggataactgt ttaataccgt
     3421 acagttaaca taggcttttt ctgcccttgt ttctgcgacc cactgatagc tcatgttgag
     3481 gacttttttc cttctcatgt gcctgctttt tctgccgccg gcaaattttg gcctctgcct
     3541 cttgtgcctc atgacccttt gcctgacgac aagataacct tgctttgctt tatatcctaa
     3601 agaacgcgcc ctgtccagcc ttgtcggatt gtctatcctt atggttgctg gctcactttt
     3661 ccactcaact atccttttct gccacaaatc gcccagattc tcttttggat tcttccaggc
     3721 ttccctgata tatttataca tcgacatttt agcctccatc aatttattta tttggattca
     3781 ggtaagctac tacctacatt tccagtagct agaagttttg gtttatttaa aaaggtttgt
     3841 taaaaaggag ggtgtgggac agtaaaccct aatcgcctag cttaaacaag ttatgccatg
     3901 ccgcggttat cccataagcg gtctctactc tgtcctccct tctctgggca aatctccatc
     3961 cagtcctttt cttatcctca gaaaatccgc tttcagactt attgcgctgg aagtattctt
     4021 caagataagc cttaatattc tcaagaaagc gatataccgt tgaacaccat tctccaaccc
     4081 ctagatgggc aatgttggac ttgggaatca ggtaaaactt ggtatttttg ccaagatact
     4141 cctgacaaag ttcgacataa gcctctgcgc tataatacct atccagcctt aaggactcga
     4201 ccttgatgcc taattccttg gccatctttc gccatttcgg ataaacccct ataatcttca
     4261 acattgattt tgtctgctca cttaataaat cccatttttc aagtatcctt ttttcctcag
     4321 cttccataag tcacctaagt gcaattaact cgcacagttg attgctcacc ctgtttaatc
     4381 tttaagtaaa agaaattagg atcgttatca atgatctcta tgctgcaatt cttctttagg
     4441 gtgtgcacta tcttgccctt gcgattgctg aaggcaggtg atatgaagaa ctgcatctca
     4501 ggtttaaggc ttctaattgt gctatttatc gcatccagct tgacaagtac aaagttatag
     4561 taggcgttct tctggatttt tgaccttact tcagtcatgg ggatctctgg tttctctttg
     4621 aaaagctcta taaatttggt gaattgcctg cttctctctt tatcgattag aatgagaacc
     4681 tgctcgttca tcctcttaac atcattagcc ccttgatgcg cctgttccat gataaggatc
     4741 agaaaataaa tggagtatat aagcaggggc tttctatatt ttgatatgca aatatatttt
     4801 ggaatcggaa tatattttga tatcaaaata tattttgcga acgcttaaat agcagttgct
     4861 aaccccggca aactataagg tatattgaga atcagtctct tctttgattt taatgatact
     4921 gccttgatta agtatgattg ttcccttcct gccagttatc ttcgcaaagc catctcccac
     4981 ttcttttaat atgccctcag cggttttaat caaactgcca tccctgaatt ttgcctgaac
     5041 acgtttatcc ttatatctta gtaattcagt tttttccata aatgaaactt gaaattgacc
     5101 tatttttaaa atctgcgctt agcgtctgaa aatacccagc ttttaagaaa acccttccaa
     5161 ttgcacctgt ttgctctcat cgatattttt tatctgcttt agctgagtgc aaatgactgc
     5221 cctaaatctg ctgactccct gagcagaaac aagatcacca actttcaact tttgggctaa
     5281 tccaaacctt tccttatgtg acttaattac cacaaagtta tagatatctt ctcctttctt
     5341 taacttcagt cttatgttgc cgttcttcgt ctttccttta tttactatct gtcctttaac
     5401 cctggctctg tttctaaatc cctttatctt tttctctccc tttgcaagct ttgacagagt
     5461 attaataagt gtcttgtcga tcatataaga gagcaaaggt tggggtgtca gcctctttcg
     5521 agaataacac ggcatttgct ccatatagaa ctttaggcat gaagtattaa tcttttctgt
     5581 tttttgctgg catagcctat agataaaggt gaggcgatag aaatatcgca caagtaataa
     5641 ccgctttgga aagatttcag gaaagagtta atcaaatgtt tattctttaa agcactgtcg
     5701 ctaacaagtt ttacataaat tcatcaaacc ttggcggttc acgcgcagga tagaactgtt
     5761 taagtgcttt ttctatctta tccgctggag aagcaacaaa taatatttta tagcttgtac
     5821 tcgtattaat agtatccgtt aagtccatat cccctggatc agagattgca actgccaact
     5881 cgtttttctt ggtaaaccct aaaggcaggc agttatgatg gggctgcagg ccattgacca
     5941 atcctgctgt ttcttgtata aatctactgc ttggctggta gctgcttagg tcatcgtatg
     6001 gagttctata tcgataagca tgtgcttgag cagcctgaac atccgttaat tttttcccca
     6061 atcttcctat tgggtctaac accgatgcag tcaagccttc ttcttcttgt tcaggtttct
     6121 taaaggcagc agtgatatct ataggtgata tcccttgatc tcgtaaccat ccctttaacc
     6181 ttttttcgcc catcttaact gctttgggcg gagagtatgc aggggacaga gagtatgttt
     6241 tgccataaaa aactaaattt cttggcgttt tcttggcatc aacctcaacg taacctttgc
     6301 cttcagccaa atgatatctt tccataaaag gcagagttac tcagggattt attaaattta
     6361 tgccaaaaat ttgtcctcaa ttgatgatta aattactcag gtaaagttaa atcagcactt
     6421 tgtttacaga aggcccagaa gtttctctcc gatatctaac agatgacagc attaagaaaa
     6481 tcaagctaca gcccgataat accactctcc ctgccttagg gattcataaa catctctagc
     6541 tctataccct acagcacttc caatccaggg cgctaacatg cttctggcat cggttgattc
     6601 tgcaacaggg catggcctta aacctggagc ttgcaggatg taaggatgtt tagtgccttc
     6661 ccttactgca atccctaact ccatgaccat atctactatt gttgacagtt taatcccatc
     6721 tcttgttttg cctgccattt tagatattaa gcttcttcag ctcctccagc cttttcttaa
     6781 attcagctaa tctctcattt gccatctgtt caacaaattg cttaacctga atcttatgga
     6841 taaaacagta ttgcttgatc agcacccttg tctccctttc aacctgcaat gttgaaggtg
     6901 gcgatatcat tttccatttt tcatactgct actgcatgca ataccgtttg tattgcttcg
     6961 cattccatct gatgcaccct gtttctgaat atctcaaagg tgtcgctctg caatcttggt
     7021 acggatctct gaaggataat gtccccggtg tccaacccct catccacata gtggatagtg
     7081 cacccacttg tgtttactcc tgcattaaac gcatcctgat agccctttgc gcccttaaat
     7141 gcaggcagaa ggcttggatg tatgttgata attctgttgg agtattggtc aacaaagtat
     7201 ttggtgataa gcctcatata gtgggcaaag atgcagaatt gcacgttttc cacctttaga
     7261 aagcttagaa cagcctgctc atggtcaatc ctgttggaat aattgttttg gtcaaagcaa
     7321 gcccatttta gattgtgctg ttttgctatg tctattaccc tggaatcggg tttattggtt
     7381 acaatgcagt gtaccttgat attaggataa gacttgccta ggatagcact gacaagcctg
     7441 gaatctcctg ttgaaaaaat tgctgtcctg cacattttag caccccctaa aaataaggca
     7501 ctatctgatt ggctgttatt gcacatattg gcactgtgcc gacaaaaggc gcatctctta
     7561 ggacaatagc tcctgtttct gttcggtatt caaggcttgt atcctttagc cctatttccc
     7621 tgacatattg ccttgcaacc tgccacagct ctgcaagcaa gtctcttacg cttccttgtt
     7681 cctgtctagt ttcagcaagc cttgtttcca acggcttttc cacccttagt ggttccaaca
     7741 cacaacttcc atccatgctc tcagccccca aaaattgata agaatagggt agcgtcggta
     7801 tcttttatag ctactgaccc aattcagggc agaatttcgg aatttggtct gatttcagac
     7861 caaactgaaa caaaacaggg gtttttggtc tgatttccgg gcagaatttt aggggccttt
     7921 ttaagaaaaa tagtgataat ttctacttta gctttctgac tgcttcaagg aaattagatc
     7981 tatagttcca tgtgtcactg taatcattga ataagcgact ttgctttagc tgaggataga
     8041 cactatttgg aaaagctgtt tttatagccc gctcatccca tctctcataa gaaccgctag
     8101 cagcataagt gtcttcatgt ataaggtctg tatgctgggt aagtattctt gtacctggca
     8161 gcggcgacaa catagcaaac ttaatagcca ttattgtttt gctatcagcc atagatgcaa
     8221 aataatccat ttcctcaaat gtttttcgaa gtgtttcagc agtatcgttt ggcgatataa
     8281 tatagcttat ttcaaagaca aatttcatat ttgaattttt tactcttttt acaacatctt
     8341 ctatcccttt tctttctgca tccagcatgt cctgagactt aacaaccccg cagcattttt
     8401 cccccaaaaa tgaggctgca tgtgctgttg cagtttccct tcctatacca tattgcctaa
     8461 tgccccattt ggatgtatct tcgataactt ttcccggctc aacaaataac gctgaatcat
     8521 aaaacacaat cctccaaggg gtatgcccta atcccgcttc gattatttcc atacattcag
     8581 atgtatgatc cctgtttgct ggtgcaaaag catttggatc acacatataa tgaaggttaa
     8641 ctttttcctt gctccctcct aattctgata caactgcatc cataccttct ttgacttggt
     8701 caaccgtgaa tcttaaaggt acaccgtttc ggcaaaaatc gcaagcattc tggcaggcat
     8761 tagcaaacat gcttatagta agttaaatag aaaagttctt atataagtac ccattctgta
     8821 attagcaaga ggtgatatag atggcattaa gaaccaaatt tcatcattcg tcaagtattt
     8881 ttaagagact gtataacaaa gaaaacaagg ttaaacaaaa aagaagatat cagaccatgt
     8941 atctcttatc caa
//
LOCUS       DASRUK010000109        40600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2254479, whole genome shotgun sequence.
ACCESSION   DASRUK010000109 DASRUK010000000
VERSION     DASRUK010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 40600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40600
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2254479"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3468
                     /locus_tag="VFF28_04735"
     CDS             <1..3468
                     /locus_tag="VFF28_04735"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZX44962.1"
                     /translation="SCKECVVPVIMVHGFTGNPKTWDNMDEYLTDKGYITYRYDYSPG
                     LGDIKVAAGGLSDFVDSIKKEIGVPKVDLIGHSMGGLVSRWYAKAHPENVRKLLMTGT
                     PNHGSSRFNAAKWLMYGGAMLGGFPAKNVKIIETAIAQSTARKQMQPCSDFLNTLNYG
                     RADVDDCFASEDNIGVETWTMAGYKGSLVWQRSVGPNDNFVSVNSVKLNHSNNKLTYL
                     SHGELNKDEHTFLWVDQILTGAVQSSMRAMSKSSAMSYQTEDYQELPMIEGHVEAGEV
                     RSYTFDLYSISAAEISLGYIGEEVEFSLRRPDGLEESNLTEEGNIKTYSLNDPIEGEW
                     QAIVTGGSSDFGIFISVESAISIKQATDKLYYAKNEIVNVSVLLESSGSPITSSGVNA
                     EMLLPDAKKQTMILLDDGLHNDLLANDGIYGYAFDETSIPGTYEVKVNASGTYEGKPY
                     TREIRFSFWVLSQPDLLVKDIYLPNIDGENITIEASIKNLGEDAHNASIEAYDGDILI
                     GRIVMDIMKKATKKAIFNWSNATVGKHNISALISPFNAFIETNYSNNRLTKEVSIYPE
                     SIYLNEQSEIMLEFEQKEDKTAFIKVPRGMSLKQGQFDVRFIAENPERVVKLWDRYYR
                     FGKGGHCTAVKIADQGYILTGSPSENIYLLKTDEEGNADWNKTFDWAGDDGENSVDVT
                     SDEGYIITGFTNSYGAGNYDMLAIKTDSYGNIEWNKTFGGQNMETGFHAKQTDDGGYL
                     ILGYTLSYGTNGEIYLVKTNATGDMLWTKTFGGNHIDRGHSLEPTSDGGYILSGYSGS
                     YSSGNIDLYLLKINDLGEEEWHKIMGGKGTEKGYDAKQIEDGFIIAGYTNSTGAGLYD
                     MWLIKTDNFGNALWNKTFGGKGFEIARSLIITEDGYLLSGYTSSFSGNYDAYFVKTDN
                     QGNIEWNKTFDTGLDEQIWETTYSDGYYTACGKASEDAYLVSFYAQQMKVDALNDGST
                     DRRLFPSYSAKISISGKESLVPISISSYKGFAEITNINLAFSYLNAYNLSLQNGWNLI
                     SLPLMQVNNSICHLFKNLAVFGYDGTWQYYYNESANNFRELEPAKGYWIKSSEPQELS
                     IAGEEYENEVDIKLKAGWNLIGYPYLTEGNINGNAFSYNNSWHRTNVLEPWKGYWIYS
                     RNDTNLSIE"
     gene            3519..4415
                     /locus_tag="VFF28_04740"
     CDS             3519..4415
                     /locus_tag="VFF28_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44963.1"
                     /translation="MNKRGQIAVFVIIGIVVLVLVGLVLAAKSSIDKKVSERETAKAG
                     EIAFEVGPIKAYAQGCLKMAGENGIWILGLHGGYLNTSVDNRYKEEGTAANMVYLGTD
                     IPYYLGAGGSVLELSQIEQKLARYVIVKFDECMALNNFDEDGFSLERPIIDYAGIGFN
                     FSAINVKSNVSINQDNVVVNVAYPMKAKKKEFEARLSEFRVDLPIRVGMIYSLIADNI
                     LTNISNSWANGQLFDASAINCNEYDISNKTNIYSISMGNDGIVRIIDYYPYYHKYTKS
                     FIFQFAVNNNPQAVSEGKCTDH"
     gene            4400..5491
                     /locus_tag="VFF28_04745"
     CDS             4400..5491
                     /locus_tag="VFF28_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44964.1"
                     /translation="MHRPLNNIYIGILLIIIASYSYAVEDDADKDGIPDATDKCLAGS
                     ILPVDNYGCDCSQKRACSGDKWCCPAGAACIDHESSARCAEDSDKDNVLDIGDRCLGT
                     KQGFATDEYGCSCEQKECDDNNPCTDDQCSPASVQCTFAVDNTNTCGQNNACQEGVCI
                     YQGYEEYYYYVYDADQEKLSIYYKHSVHVIDVVFSEPLLEDIIVIDSTSEVVDYQLQD
                     DRKKLTVTVQGKDGTEGQTSIKTKQEPKSVSIDSRQIPKINEKLSPKSPTWLYIISIA
                     MMFGIMLIVVFSLRRHEDVVMGTIKKEEDKIEREVSLEAELQLKMYIMTNLKRGYQAL
                     QIRNELIRDGWKPHVVDRVFQSMSGRFYK"
     gene            5510..6013
                     /locus_tag="VFF28_04750"
     CDS             5510..6013
                     /locus_tag="VFF28_04750"
                     /inference="COORDINATES: protein motif:HMM:NF024635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase family protein"
                     /protein_id="HZX44965.1"
                     /translation="MSIIAITGSVCSGKTTLSKLLARKLGFRYISGSEVVEKYKLSLG
                     YDRKRKCKIIDEKQFSKAVLKEIDGKDAVVDSHMSHYLPAKHVDLCIVTKCDIKEMKK
                     RLSKRNYSQLKIKENIECEIFDVCTSEAREFGHKVLVISTTKGINIEEILGRLTKNGI
                     KPSSNRT"
     gene            5982..7262
                     /locus_tag="VFF28_04755"
     CDS             5982..7262
                     /locus_tag="VFF28_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZX44966.1"
                     /translation="MELNPQAIELNNAIKAANTNVNEMLSEKGKAIFFPKKGILGQGA
                     EAKGKKINATIGIALEEDGSPMRLKSISKRVKLEPGDVFPYAPSFGKKELRDKWKEML
                     YKKNPGLLGKELSLPIVTNALTHGLSMVGYMFIDAGDKVILPDQYWGNYRLILSNAYG
                     ALLQTFQMFSGSGFNLDGLRESLQGEGKKILLLNFPNNPTGYTPTIAEASAIREIILD
                     AAKQGQKIIAIVDDAYFGLVFEEGILKESIFSMLCGLHENVLAIKLDGATKEDYAWGL
                     RVGFITYGTLDGNRQLYEALENKTTGAVRGSISNCSHLGQSLVYHAFSDPSYWNEKAN
                     KFKILNRRYKEVKKTLKNKSYGEEFSPLPYNSGYFMCIQLKKKDGEHVRKILLDKYDT
                     GVISLGNIIRIAFSSVRTEDIKQLFDNIYNACRE"
     gene            7264..7815
                     /locus_tag="VFF28_04760"
     CDS             7264..7815
                     /locus_tag="VFF28_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44967.1"
                     /translation="MAKSNLRDKLHYIFGHIMAIVALVIVVIVFGGAYYFIIAPNLVS
                     KPSIEKPAFSENAIEEMSEGKQAISPEHINYLVNELDGYKLKNTWGDKLPIIEFLFTD
                     TNVRYYSYVKDHVPITKEGNAKGNDILIKGSQQVAYEIIESGNIPQATKQAVDSGKLS
                     VELVSDMKTLATKGYLSIYDNVR"
     gene            7812..8180
                     /locus_tag="VFF28_04765"
     CDS             7812..8180
                     /locus_tag="VFF28_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HZX44968.1"
                     /translation="MKIRIRCGQIDKEAQLDDSSTAKTAYDNLPIKGSIHRWGDEIYF
                     DVPFHIGLEVDAREVVFKGEIGFWPEGDCFCIFFGKTPMSEENEIRAASKVNVFGKII
                     GDSTVFKGALEGQSILVEKI"
     gene            8233..10074
                     /locus_tag="VFF28_04770"
     CDS             8233..10074
                     /locus_tag="VFF28_04770"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013466483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HZX44969.1"
                     /translation="METDNESLRKLKSLGNKHVESIVQQYIEICRPDEVIVFDNSEDD
                     ILRARKLALNGEERALRTKGHTVHFDGPKDQGRDLINTCVLVPKGVKLSEAIKTIDRE
                     EGIKEVMGILEGIMRGKTMFVKFYCLGPNNSKFSIPALQITDSAYVIHSEDILYRQGY
                     DEFKALKGSKNFFHFIHSAGEIENNVSKNIDKRRIYMDLIEERVFTVNNQYAGNSVGL
                     KKLALRLAISKANKEDWLCEHMFVMGARPEGKNRVTYFTGAFPTACGKTSTAMIPGQT
                     IIGDDIAYIRPDEEGTARAVNVEQGIFGIISDINPKDDPLIYKALTTPRETIFSNIFV
                     KDDMPYWNGMGKEIPSEGVNFSGEWSKGKKDSKGNDIPPAHPNARYTIGINELDNADS
                     KADSPIGVPVSGFIYGGRDSDTTVPVLQSLSWQHGVFIGAALESETVTGTTDKAGVRK
                     FNPMSNIEFLTVPLGVYIKNHLTFGEELDKPPLVFATNYFLQKDGKYVNGKLDKKVWI
                     LWMEGRVHNEYEAIETPIGLLPKYNDLKKLFRAVFSKDYSREEYEYQFSVRINKLLEK
                     LDRIELIYKVEQDIPDIFHEHLNQQRQRLKSAREKFGKDVISPFEFE"
     gene            10106..11197
                     /locus_tag="VFF28_04775"
     CDS             10106..11197
                     /locus_tag="VFF28_04775"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HZX44970.1"
                     /translation="MKAGLASIEIHYLVEELQFLIGGRVDNIYNPSNEELLIQFFVSG
                     KSKQMLRIISGKFMYLASEKEEAGSPSGFCMLLRKHLGNAKLRSIRQIEPERIVEMLF
                     EKDEIKKVIVELFGKGNILICDKEDNIISALVYHKFKDREIKQGLKYSYPQMGINLFD
                     IAEKEIEKVFAESGNTLVKTLATCLGLGGTYSEEICNISGVDKNKKASEAKQEINPIN
                     KALKRVIKGEKEAAIIFKDKEVKDIVPIKLNIFEGYEQKVFKSYNEALDHYFLSVKEV
                     PKNPQIEKLKRIIVNQEEKIKSLEHEEITERQKGDYIYQNYASIGRIVEEIKEVVKKE
                     GWGGLEKKAEGHKIVKAVKAKDKTVEIEL"
     gene            11241..12107
                     /locus_tag="VFF28_04780"
     CDS             11241..12107
                     /locus_tag="VFF28_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44971.1"
                     /translation="MALGRELDRYISGKRKRESFSSFKERVLNKKSALSNKMFDRLGA
                     ITQGGEAAKPDVPEDEGWKEVLLEGSAEPETVIDEPVIKQAQPAVNKEPVQRPTTQER
                     NIPHTQPPARETNEETAEEIRESNKGRLEQLRIARKQERMGALRGKVFNILFKKKEKK
                     EKDMAEQVRTEIRLQAKKEEASKRAIIPEKPKIVEHKKSFFSNFIEIKTAEEIAEDDK
                     AAAMQEQAVANVEEINNLLSGQPGEIRTNCPAQNPLEFPELFPARKESAIEVGKVISL
                     DNDYKIKVIRNN"
     gene            12148..12261
                     /gene="rrf"
                     /locus_tag="VFF28_04785"
     rRNA            12148..12261
                     /gene="rrf"
                     /locus_tag="VFF28_04785"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            12344..13651
                     /locus_tag="VFF28_04790"
     CDS             12344..13651
                     /locus_tag="VFF28_04790"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HZX44972.1"
                     /translation="MKIAVMGTGYVGLTVGTCLAELGNEVICVDVDEKKIKSLNEGKI
                     PIYEPGLEDMLKRNFKEKRILFSTDSKTAITSSSVIFIAVGTPPGKDHTADLSFVKQV
                     AKNIGSHMNAYKVIVNKSTVPVGTAQLVSQIVKESQRQAMDFDVVSNPEFLREGEAIN
                     DFMMPDRIVIGADSEKARKVMENIYIGIARTSKPIMFTDTKSAEIIKYASNAMLATRI
                     SFMNEIARLCEKAGGDVKEVAKGMGFDSRIGPRFLQAGVGYGGSCFPKDVKALEQTMN
                     SYNIKSQILKAVEQVNEEQKKSVLPKIKKILPELKGKTIAVWGLAFKPKTDDMREAPS
                     LVIIDQLQAEGARIKAFDPEAEATAKLVLKDVEYAGHPLEAIEGADALIIVTEWNEFR
                     NLDKEKMIQLMKHPNIIDGRNVYEPKEMNALGFNYMGVGRGTE"
     gene            13648..14382
                     /locus_tag="VFF28_04795"
     CDS             13648..14382
                     /locus_tag="VFF28_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZX44973.1"
                     /translation="MKAIILAAGYATRLYPLTKDKAKPLLSVAGKPIIEHIISKVEQV
                     DEVSEIFIVTNDKFAEQFEKWALGFRCSKKIKIVNDMTKTNEDRLGSLGDVDFVLEHE
                     KVKESILVIAGDNLFHFSLREFVQSHKKHNKSAVALYDVNDMELAKQYGIVGVNKENK
                     MIEFAEKPAQPKSTLASTGVYIYPPHVLPMLDDFVKKFKNSDKAGNFLEWLHKEEHVY
                     CYITKDKWFDIGTHEQLAKAQEEFKE"
     gene            14497..15426
                     /locus_tag="VFF28_04800"
     CDS             14497..15426
                     /locus_tag="VFF28_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HZX44974.1"
                     /translation="MKTIVVSGGAGFIGSHLCDSLINSNKIICVDNLFTGSKANISHL
                     LKNPNFTFIKHDTIDPLFLDDLKIDQIYNLACPASPVHYQFNAIRTIKANVLGTTNML
                     GLAKKHKARILQASTSEVYGDPLEHPQKESYWGNVNCTGIRACYDEGKRCAETLCFDY
                     HRQNKVDIRVVRIFNTYGPRMAKDDGRVVSNFITKALQGKDITVYGDGSQTRSFCYIS
                     DLIDGLIKMMNNDEFVGPVNLGNPEEFTILELAKKIIELSDSKSKIMFEKLPEDDPRL
                     RRPDISKVTARIGWRPKVRLDKGLTRTIDFFKK"
     gene            15423..16589
                     /locus_tag="VFF28_04805"
     CDS             15423..16589
                     /locus_tag="VFF28_04805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZX44975.1"
                     /translation="MRICILSTTYPRYKEDAWVPFTHSLSKELAKKHQVTVVTSSDIA
                     AKDYEKRDKVKIIRFIYFLPKKWQMLTYRGGMLESFNSSLLAKVQAPLFLLSFFLKAL
                     KHSRRCDIIHAYWSLSGLIAVLVSKIRKKPIVLTLHGSDIRSLPRWLNRYVVNNVDAV
                     VSAHHELLDIAEKLGRKEELYNIKNPIDSKFYSKIKTDGVKKEFGLKVEYVVSFIGRL
                     EPMKDPLTFIKAIPFVLAQMNGIKFFLVGDGHLMQEAVRLAEKLKVKEHLFIIGQRHD
                     THEILKATDIFIAISPLENCFSTTIIESMSLKVPCIITDAGYTKKFFTHRKDCYLVKS
                     RDEKELANAIITLLLEQDLRRKIALNGLKFLDNNKFNNSQIIKGYQAVYKSVFT"
     gene            complement(16582..17319)
                     /locus_tag="VFF28_04810"
     CDS             complement(16582..17319)
                     /locus_tag="VFF28_04810"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZX44976.1"
                     /translation="MKSQKYKGELYQKVRWRNTLSGRIYERLHSSNSFLGKLYKSTFI
                     CPIYPNLDDKALLNKICSKESLILDIGGNVGSFDMRKYKNYFRLDIDDSARPDIVGDA
                     LCLPLKDNSTDIIIAKAIFEHIKDPFQTVREIRRVLKKGGILFTLVPFYYRIHASPRD
                     YYRFTKDGLYYLLKDFKHTEVKPCGGYITSLGNCFFTGTYALDTLFGMGFLIRVLAWP
                     ILRTLTYLDAFDKFNLTATYFYSVSVK"
     gene            complement(17300..18403)
                     /locus_tag="VFF28_04815"
     CDS             complement(17300..18403)
                     /locus_tag="VFF28_04815"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZX44977.1"
                     /translation="MKVDFVDLKRQYASIKEEIDAAIKHVVDNTDFVLGKEVEAFEKE
                     FASYCGKKLALGVNSGTAALHLALLAYGIGKGDEVITVPNSFFATAEAISLAGATPVF
                     VDIEEDSYNIDVNLIQKAITGNTKAIIPVHLYGQPCDMDQIVKIAQEHNLIIIEDCCQ
                     AHGAEYKGRKVPVSATGCFSFYPGKNLGAYGEAGAVVTDDEGVAEKVKLLRSHGEYPK
                     YNHQMVGYNYRMEGLQGAVLRVKLKYLESWNEQRRKNAQRYNELLKKVIIPIEKEYSK
                     HVYHVYQIRVKERDMLQDFLKASNIFTNIHYKTPIHMQKAYSSLGYLPYDFPITNKVV
                     NEIISLPMFPGLTKEEITFVTTKIAEFYEKPKI"
     gene            complement(18400..18879)
                     /locus_tag="VFF28_04820"
     CDS             complement(18400..18879)
                     /locus_tag="VFF28_04820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZX44978.1"
                     /translation="MTQLIRDDVKLGKNTKAYNFVNLYGCEIGDNTKIGSFVEIRKDV
                     KIGKNCKIQAFAFIPEGITIGDEVFIGPHACFVNDKIPRATNKEGNLKEEGTDWKIES
                     THVKDGASIGANATILCGVTIGKNSIVGAGAVVTRDIPDNVIVAGNPAKIIGKVTDR"
     gene            complement(18876..19907)
                     /locus_tag="VFF28_04825"
     CDS             complement(18876..19907)
                     /locus_tag="VFF28_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZX44979.1"
                     /translation="MVNVGVIGCGYWGPNLIRNFSQIEDCSVIGCADLSQDRLKHISK
                     AFPKIKTMTDHKELLNDDEIDAIAIVTPVSTHFKLAKEALEKGKHVLIEKPITATSDE
                     ARILIDLAKEKNKVLMVDHTFEYSGPVSKIKELIDNGDLGEIYTVDMVRVNLGLFQQD
                     INVIWDLAPHDISVLIYMLGGMPSSVRAIAESYIQPKVEDSAYITLKFKSKTMANLHV
                     SWLDPCKIRRTTLVGSKKMLVYDDIELSEKIKVYDKGVTFETIDKAKPKHWDSYKEFQ
                     YMYRDGNINIPKVDLKEPLRSMTEHFVDCIKSGKQPRSDGNSGLQVVKVIEAAQLSLK
                     NKGTEVQIA"
     gene            20059..21018
                     /locus_tag="VFF28_04830"
     CDS             20059..21018
                     /locus_tag="VFF28_04830"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HZX44980.1"
                     /translation="MRGEAKSNIINTLLRNKHEQLSKYKIAKLAGCSYSWVHEYLDEL
                     EAKRILKGTKVIDEAALKGQLALGEKKERANMPILIILAAGVGSRSLPLTKDIPKCLM
                     KVGGKTLLERMIGQNYASSLTSVLVVVGHKNELVEEELARLKKTYRKLKAKTLFNSDY
                     LTTNNIYSLWLARESAKNGFILVDGDVIVDDAIIRKVNESNIPNLLLIDEDKAPEEED
                     VKVLMDSSGIYGIGKHIELNKSNGISVGIAKFSREGAKVLFDEVKKKLDEGKRNIFYE
                     EAFEAMTSKIRIDTITTGSAHWREIDFLSEYEDAKKVFRYDQR"
     gene            21005..22315
                     /locus_tag="VFF28_04835"
     CDS             21005..22315
                     /locus_tag="VFF28_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZX44981.1"
                     /translation="MISAEEILNKDKEYVIRAWSSNTGPIPIVEGKGVHVTDASGKEY
                     MDFASGYFVNVCGHSHPDIIKAVSDQVSRISQVSMRQTTVPLVLLAEKLAKITPGNLK
                     KSFFSTGGAESVEFAMKMARCYTGKHEIIALRNAYHGLSLATLSASTNTSYRGTAYNP
                     LPSGISHAPNAYCYRCKYNDHGCDLQCAREIEKVIKGKSFEVVSTENIGTLLIEPVQS
                     VGGIIPPDGWFELVKEICEKHHILLIVDEIQTGLGRTGKMFACEHWGIKPDIMTLAKG
                     ISGGVGSMGVTITKDEVVSNFSSGTCSTSAGNAVSCAGGLALIETLEKEKLPENAARM
                     GQYLTKVLKEMDNPNFGDVRFKGLMGGVEMVEDRDSKEPLQKKAMSKIVGKLQEQGVI
                     ITVSGPLGNVFRIQPALNLTKEQADRFVDIFSNTVKGVCNSAKL"
     gene            22290..22967
                     /locus_tag="VFF28_04840"
     CDS             22290..22967
                     /locus_tag="VFF28_04840"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZX44982.1"
                     /translation="MFAIQPSFDQLKDALKQEDSLFSTYFYRRISLRITYLLIRTQIT
                     PNQITFFSLILGLVASFLFSLGSYYYALVGILFIHLSFLFDHVDGEIARYKKLRSDFG
                     AWFDQVSDRIKESTAFIGLALGIYLATGSSLPLILGMFAVFNLFMIGHLRGTTSQLKF
                     EHTSELKITSKVHIGSVDTTIFLITLAVIFDLQLYLLMLYATFGCLAWFYQIYTRYKR
                     STKTSRS"
     gene            complement(22964..23857)
                     /locus_tag="VFF28_04845"
     CDS             complement(22964..23857)
                     /locus_tag="VFF28_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZX44983.1"
                     /translation="MIIITIPAFNEEKTLGQVLDDINKVMKDLSYDHKILVVDDGSTD
                     NTSLVAKNHGAAVFAHPKNYGLAETFKTEIEQCLKMGAEIIVHIDADGQYHARDITRL
                     IEEVKKGNDLVLGSRFKGNIEYMPFINKIGNRAFSAVISGITKTKITDAQTGLRAFSQ
                     EVAKKVGITSNFTYTQEQIIRAIKLKFKVKEIPVDFARRKDKSRLFGHPFSYAIKAGL
                     TILRIYRDYEPLKFFTFIGGSLFGTGFLIGIWLLYLFLSSGAIRHIPSAILSMLFIVI
                     GIQILIFGFLADMIKNKRINH"
     gene            23911..25773
                     /locus_tag="VFF28_04850"
     CDS             23911..25773
                     /locus_tag="VFF28_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44984.1"
                     /translation="MGFFTILLFFVYSWGLGYSITFFLRNSDNFFERNIMRVGIGLGV
                     LPILAVILNLVRIPLDWKIMLVLSLAIPVIYLINHFRNIKLANPFKVSKSNVYIFIVL
                     LMFLALFGMYHKGSFAYPYLEDGDPWLYAVAAKYIAVHDTYSKPVDMFGSEYEISHYT
                     EPYPAAYNILMGILHQTSPSINWTLKFFNALMLSLSLVFFYFFVKRFSQNKNLALAST
                     FVMLVIPCFLGHFIFSQVLGVMLFFPALYCLAMIEDDKKWAFVSAAPIASILFSQLVT
                     AAVFAIIFAVPYFIVILFKKKELRKYFILAGLLGAALSLSYWGEMVYKFGYEGVRYQN
                     SMGSQTPFKVGGTLGDIYGWDDFIYGAYYGNSLFSIRWANAIDSPIGLGWFLFALLLI
                     SFVLVIIQARKIVGEKPWLLISTIWLILTYVNIRSGALPVGFVPHRSWTFFAIPVSII
                     AGYGIIAILSLLESKEKKALALVVVITLLLVTSGYPKYYVNTVPWPQHRLFPDMNGEL
                     GGYLWLNGLPVDTMVTSLCRTDDVVIGFDKMSTAQWDRSQIDFKKHLVNYSSVEISEH
                     LKKNKYQFAVIDSQCVERFGINETNTKLNEMVGSGLFISVLPQNFNGIVILKVF"
     gene            complement(25760..27196)
                     /locus_tag="VFF28_04855"
     CDS             complement(25760..27196)
                     /locus_tag="VFF28_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HZX44985.1"
                     /translation="MPTAKIVAKNVGSLLFADTLGRVLSFLLFVLIARYLGDVGLGKY
                     SFAFAFSGLFFIFSDLGVTTLMTREVSRDRQKAQEYLMNLVVLKLFIGSLPLIASTIA
                     IFFLAESTDVIILVMLSSITLFLDSFSLIFKNVFSAFERMEFYSAANVIERLIAVIIG
                     SILILKGYGLISLFIVLVISYSASLGFSMYVVSKKFAKIRFKMNPKFWQFFVKRSLPF
                     WFTVFFMTIYYKMDVVMLTSMKDYATAGLYSAAYKIVDAFSFISFAAVAAIFPTMSHL
                     HLNEKGKLQMLYHRSFYYLFVVGLPIGIGAVVLADKIILLIYKEPFLASVPALQILIW
                     TEVLLFVNYLMGYLLNSIDKQRLFTYTAAAAAGGNLLLNFILIPRYSIIGASIATVLT
                     QVISFLFLYHYCSANGYRVGLKKVIIKPLVAGIAMGIVIYIIRGLNLFIILPIAIATY
                     ALVLLSIKGIGNEEIDILKQFIQPFKKP"
     gene            27249..28175
                     /locus_tag="VFF28_04860"
     CDS             27249..28175
                     /locus_tag="VFF28_04860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HZX44986.1"
                     /translation="MTLGKARIFSIAAVIIAAVLAFLYLQKNPYDFKGLTFQVFLVLS
                     IFSWISAILNGIRVKIFCDIFNIGLSFKEWFGLSQVTSMGNYLTPFKGGTSLRAIYLK
                     KHYDFPYNQFIATMASTAVISFAVYGLLGLISLIFIFAATNTFNSLLFAIFSICTFGS
                     LVIFIFPHSIITRLFTRGFLAGYIAKILEGWSIIKNKPSGIIKLIAIEVLFSAAFAFS
                     IFFAFRAVGFRLPFIYCMIVAVISNISIIFQITPAGLGVREAVTVWASSLFGAGIALP
                     LNVALLVRLVAFIWVVPFGMIYGFMLLGKKRQ"
     gene            28236..30017
                     /locus_tag="VFF28_04865"
     CDS             28236..30017
                     /locus_tag="VFF28_04865"
                     /inference="COORDINATES: protein motif:HMM:NF042614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6798 domain-containing protein"
                     /protein_id="HZX44987.1"
                     /translation="MHNLFNRHKDRIKIYSILIGMVLLYMLVKGYSFYASDPYEYVPS
                     IQKFIDNDYLKNDWFLNTQTGFNVRTYFYYLISFFLPVLSIKSTFLLLFIVNTFFLVL
                     GIFYISQLLFKDRLSSYLSCFLVLFSVWKLIGDFAIIAPFFTPSHISITLSIWALFFF
                     LRKRYNIMFLMLGLSTFLHLLIGISALILLSTVILVRDRFSSIKIFAVGSVFYSPALL
                     SIIPLLLIEFSQRSSASEVILTFAWIRIPWHLIPSQFSLISYISFSILTLLFLYSLRD
                     AGKSKMNHDIYLMLICAAIYFFLGYLLVEVFPIELAMKLQFFRMIILVRIILLIYIAN
                     HFSKSFASSPSVLLAITLASLFDHILIVIVPFYLIYELFIKKKFAGYTSTMSYACLAS
                     IGLTFFAFILFGKNFISSEFFRRIFSDYLWIFFILAVYYIHTLRKMKIYKLVIPSVAI
                     AFCILSIAVIAQFKSLEPEKDNMYSWIRQNTPDDSVFLIPPYLEDFRVKAMRAIVVDF
                     KSMPFQSDQMIEWRNRLSDITSNIEFRSAINRKNELKVGFESLTKEDIARLQDKYGFQ
                     YILIEKPMRLQQKSIYENAKYALYSTS"
     gene            complement(30007..31320)
                     /locus_tag="VFF28_04870"
     CDS             complement(30007..31320)
                     /locus_tag="VFF28_04870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZX44988.1"
                     /translation="MKARGYGNLKGAESLSVILPSYNEQNNVGELIRRVKSQLQALNI
                     KFEVIVVDGGSRDNTVKEAAKEGAKVIIQKSKGYGGALLEGFALAKGDYVLTMDSDMS
                     HDPVFIKNLWMQRSYADIIIASRYVKGGKAKMSLSRTFLSKSLNAFFARALSLPLKDL
                     SSGYRMYKKEVVGESLQLKGTDFNILQEIVVQAYNNGWIIKEEPFYYRPRKFGKSKAK
                     LAKFACSYLITLGKMWKMRNSIEAADYDDRAYNSKILPQRLWQRARHKVIMSFLAQSF
                     RPSEKPLIVDIGCGSNKLIQQLNNSIGIDIMLKKLMFLRSKNKNLLQASIFNLPLKSD
                     SIDCIICSEVIEHLQKGEQPFLEITRVLKRGGILILGTPDYSRASWHIIEWIYSKILP
                     NAYADEHISPYSRKSLKKLLGKHGFNVVGSRYVFGSELILRAIKK"
     gene            complement(31283..31654)
                     /locus_tag="VFF28_04875"
     CDS             complement(31283..31654)
                     /locus_tag="VFF28_04875"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZX44989.1"
                     /translation="MEKVVISCPRPSDYLRMYHLSNKERFTKWGFFKQVVLSPVVPGW
                     DILVAKANGDLAGFIFLRDRSIYRLCVNALHRGKGIGKKLIPSHALFSETNRKDAVKF
                     WEASGFRMVRESKGVWKFERR"
     gene            31745..32863
                     /locus_tag="VFF28_04880"
     CDS             31745..32863
                     /locus_tag="VFF28_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZX44990.1"
                     /translation="MSRKILVTGGAGFIGSFIVDKLIEQGNDVRIFDSLEPQVHGDKM
                     PDYLDPKAEFIRGDVNDAGALKDAVSDMDVVFHMAALVGVGQSMYQIKRYVEANTLGT
                     SNLLDILVNSEHDVKKLVVASSMSTYGEGSYECENCGEVHPPLRPESQMQKNDWELHC
                     PVCDKHCKPIPTKESKTQEVNSIYAITKKDQEDMVLNIGKTYGIPSVALRFFNVYGPR
                     QSLSNPYTGVAAIFMSRIKNANPPIIFEDGNQSRDFISVHDIADANILAMKHQSADYE
                     VFNVGTGNKISILEVAETISRLYKKSIMPKLAYKFRKGDVRHCFADISKIRDKIGFDP
                     KVSFSAGMGELIDWAGSVDAVDRVDDATDELQKKGLLS"
     gene            32911..33861
                     /locus_tag="VFF28_04885"
     CDS             32911..33861
                     /locus_tag="VFF28_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005548569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HZX44991.1"
                     /translation="MKIFITGITGFVGSHMAEFLLREKHQIFGLKRWRSPLDNIKGIL
                     DKVELADGDLRDSKSIYDIIAEIKPDRIYHLAAQSYVPTSFKAPNDTIETNVMGTINL
                     LEAVRHAKIDPRIHICSSSEVYGQVTEGELPIKETNPLRPQSPYAVSKVGEDLISFQY
                     FKSYGLKSVRSRAFTHTGPRRGSVFALSSFAKQVAEIEKGHQKPVIHVGNLESIRTFL
                     DIRDMVRAYYLLLERCEPGEVYNIGGNATMTIREMLEKLLTFTKVKPEIRVEKELLRP
                     SDVTLQIPDVSKFKKATGWEPDIPFDKTIKDTLDYWRENV"
     gene            33941..35551
                     /locus_tag="VFF28_04890"
     CDS             33941..35551
                     /locus_tag="VFF28_04890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZX44992.1"
                     /translation="MDETAYIIQGYLILTKGIFFSDDTMPFAKVISGLPLFLLKPNIP
                     SGYYDLYLDNEFMKLGADFLYKSNSNSGIMVFFARIFPLLLSLLLGFFVYRWASDLYG
                     TKAGILSLILYTFSPNILAESASATSDLAAVCFMFGSTYFLWRYLKFEKKTDLLVTSL
                     LFGLAQVTKLTALLLIPVFVLFLAYPAFKARSFDKAKKAAIAFAVILVASYIMINMAF
                     LFQGTFTPLGKSLADDPVHYFDKAKFNSESISQGFPAWARNAGSYYLNNVPTFIPYPY
                     LKNFMGAVRKYENPIFLGGYLNGKLHENGDNYFYYLENFLLRTPIALILLIILSIALS
                     KKYTDSIGDNIMLLIIPIALFLYFSSGTMWGGIRYLLPIYPFMFVFVSKLANSREKKI
                     IFPVIALAAWFVMGSILAFPSHPAYFNEAAIGEGHFFLSGPSFDVGQDLKALGNYAKA
                     NSLKGLKLAYFGEGSPDYYGLNYSYIAYYGNLKQSVENCSRQEGLVAISITYLHPTKQ
                     LNNPDCFDWLSGYKPVKKIGNTIFLYEV"
     gene            35553..36668
                     /locus_tag="VFF28_04895"
     CDS             35553..36668
                     /locus_tag="VFF28_04895"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZX44993.1"
                     /translation="MKIPVCEPKLSGNELNYVTDCIKTGWISSLGDYIAKFEDKFRSF
                     TGSKHAITCSNGTAALHLALQALGIGRGDEVIIPDLTMIAVPYSVIYTNARPVLVDAE
                     MDTWNIDPEKIESKITGKTKAIIAVHTYGHPVDMDPVLEIAKKHGIYVIEDCAEAHGA
                     LYKGKKVGSIGDIGCFSFYANKILTTGEGGMVVTDDASVEKKIKLIKNMAFAEPRFSH
                     FEIGFNYRMTNLQAAIGLAQMENAEELIDERMKVCRIYDSLFKNIPGIVRPPQNAWAK
                     NVYWLYSMLIDDSFGASRDEVKRKLMEKGIDTRNFFTPMHRQPLFMKKDERFPATSGL
                     FPVADKLSRTGIQLPSGDNLSKEQVQTVVEEILSLAK"
     gene            36665..37372
                     /locus_tag="VFF28_04900"
     CDS             36665..37372
                     /locus_tag="VFF28_04900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZX44994.1"
                     /translation="MKSISVILPTYEEKDNIKDMIQRIKRSAGNLKEIIVVDDNSPDL
                     TWKLVSELAKRDKKIKLIRRIDERGIASAIGKGIKAATGDIIIWMDCDSTQPPELIPR
                     LVERLDHYGLAIASRYVGIGSDKRTMTRIVTSKLFNIFASFVLWLPIKDTDSGFIAVN
                     RKVFDAVPFPAYGYGEYFVEFIYKCHKKGIRMTEVGYEFRDRSRGRSKTADDIPTLLR
                     YGWQYGIKVLKLRAGLI"
     gene            37369..37725
                     /locus_tag="VFF28_04905"
     CDS             37369..37725
                     /locus_tag="VFF28_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44995.1"
                     /translation="MKIIDVPFEHSDERGIFRELARGDKWKEINVAERKKGITSGNHY
                     HKVKDELFYVYRGRCHLRVLNFKTGKEDNYDFKENDMFVLEPYEMHEFSYSEDTLFVI
                     LLSLAFEQGNSDTYTL"
     gene            complement(37747..39165)
                     /locus_tag="VFF28_04910"
     CDS             complement(37747..39165)
                     /locus_tag="VFF28_04910"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZX44996.1"
                     /translation="MTKIVLISPPFGEEESAGKTESMKAVLNIIPPLGLCYIAAVLEK
                     NGYDVSIIEGQLDMTYETVIKEAIRQKPDVVGMSILTPMLGMVKKIAQNIKKELPDTL
                     VVVGGSHVTALPAQTLQAECFDAAIIGEGEYTFLDFVNDHEKNNSANLAQIKGLAFKK
                     GGHVVINERREFIKNLDELPFPARHLLPPLSKYRPTPASYRKLPATHMMTSRGCPSLC
                     TFCDRSIFGSTYRERSPENVVAEVEELVNKYKIKEIKFFDDTFTLNKKRLYSIFALMK
                     ERGINIPWTCLTKANRVDEEMLTAMKNAGCWQVLFGFESGNPQVLASLKKGTTIQDNQ
                     RAADLCRKVGLSMRIDYLCGTPMETMETMRQTLEFAKKINSDFAHFNKFTPLPGTELY
                     HNLLKEGYKFDIEKYCSQLDHSEVIYAPSSVDKQEYAKFLDKSYKEYYLRPKYIAKQL
                     ANIRSIEDMKRMWRGFRAITQL"
     gene            complement(39158..40324)
                     /locus_tag="VFF28_04915"
     CDS             complement(39158..40324)
                     /locus_tag="VFF28_04915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HZX44997.1"
                     /translation="METKRDCRFFKGSKPCAYHKSDGSVCASCRFYDGVKTRILVINL
                     VGIGDVLRTTSLLEPLKAKYEGASIVFLTSQNVYDLLKNNPLIDELLALNLESSLRLQ
                     ASKFDVLINLDKSAEAAALSCLIRADTKLGFGLKEDGLIFAMNPGSQAAMDMGLSDEL
                     KIANKKTQQQLMFEISEMEYNGQRPTLILSKEALDNAKKFLVKNNIKSEDLVVGFNTG
                     CSKRVFPNREWTIEGFVGLAELLYNNLNARVILLGGPSEQEKNREIALKSQVPIINGG
                     VHPIDDFAALVGSCDLIVSGDTSAVHIAVAQNRLVIAIFGCVNPNEIELYGDGRRIMS
                     DLDCAPCYKQNCDKDIECMRKISADHVYKEIIGLLQEKKIAVKNRIVREVSEDD"
BASE COUNT        12240 a         7919 c         9015 g        11426 t
ORIGIN      
        1 agctgcaaag aatgtgtggt acctgtcatc atggtacatg gatttactgg aaatcctaag
       61 acatgggaca atatggatga gtacctcact gataagggat acataaccta taggtatgat
      121 tattcccctg gccttggaga tataaaagtg gctgcaggag gcttatctga ctttgtagac
      181 agcataaaaa aagagatagg ggttccgaaa gttgacttaa tcgggcactc tatgggaggc
      241 ctcgtgagca gatggtatgc aaaagcgcac cctgaaaatg tcaggaagct cctaatgacc
      301 ggaactccaa accatggatc atctaggttt aatgcagcaa aatggcttat gtatggaggg
      361 gctatgctgg gcgggtttcc ggcaaaaaat gtaaaaatca tagagacggc aattgcgcaa
      421 agcacagcaa gaaagcagat gcagccttgc agtgattttt tgaacaccct taactatggc
      481 agggcagatg ttgatgactg ctttgcctca gaagataata ttggtgtgga gacttggaca
      541 atggcaggct acaaaggaag ccttgtatgg caaaggtcgg ttgggcctaa tgataatttc
      601 gtgagtgtca atagtgtgaa actgaaccat tcaaacaata agctcaccta tctatcacac
      661 ggcgagttga acaaagatga gcacacattc ttatgggttg accagatact aacgggtgct
      721 gtgcagtcct ctatgagggc gatgtcaaaa tcatcagcca tgagctatca aacagaagat
      781 tatcaagagc ttcctatgat agaagggcat gttgaggctg gagaggtaag gagctacact
      841 tttgaccttt attccatatc tgctgctgaa atctctctgg ggtatatagg agaagaggtt
      901 gaattcagcc tgagaagacc ggatggttta gaagaaagca atcttactga agagggaaat
      961 atcaaaacat atagtcttaa tgatccaata gaaggagaat ggcaagctat agtaactggt
     1021 gggagcagcg attttggaat atttatatct gtggagagcg ctattagcat aaaacaagca
     1081 acagacaaat tatattatgc taaaaatgag atagtcaatg ttagtgtctt gcttgaaagc
     1141 tctggcagtc ctatcaccag ctcaggggta aatgcagaga tgctcctgcc ggacgcaaaa
     1201 aaacagacga tgatcctgct ggatgatggc ttgcataatg atctgcttgc taatgacggc
     1261 atatatggat atgcatttga tgagacatca atacccggca catatgaggt taaggtaaat
     1321 gcaagcggaa catatgaggg aaagccttat acaagggaga taagattttc tttttgggtg
     1381 ttatctcagc ctgatctgct cgtgaaggat atatatctgc caaacatcga cggggagaat
     1441 ataacaattg aagcgagcat caagaacttg ggtgaagatg cacacaatgc ctcaatcgaa
     1501 gcgtatgatg gggatatatt aatcggaagg atagttatgg atattatgaa aaaagcgaca
     1561 aaaaaagcaa tatttaactg gagcaatgca actgtcggca aacacaatat atctgcgtta
     1621 atatctcctt tcaacgcttt tatcgaaacc aattactcaa acaacaggct tacaaaggaa
     1681 gtgtcaatat atccagaaag catctaccta aacgaacagt ctgagattat gcttgagttt
     1741 gagcaaaagg aagataaaac agcctttata aaagtgccaa gaggcatgtc gcttaaacag
     1801 gggcagtttg atgttaggtt tattgctgag aatccagaaa gagttgtaaa gttgtgggat
     1861 agatactaca ggtttggcaa aggagggcac tgtacagcgg taaagatagc ggaccaggga
     1921 tatatcctga caggatcgcc gtcagaaaat atctatctcc taaaaacaga cgaggaaggg
     1981 aatgctgact ggaacaagac atttgattgg gcaggggatg atggggaaaa ctcagttgat
     2041 gtcacctccg atgaaggata tataataacg gggttcacga actcctatgg tgctggaaat
     2101 tatgacatgc tggcaatcaa gacggatagc tatggaaaca tagaatggaa caagacattt
     2161 ggcggccaaa atatggaaac aggttttcat gcaaagcaaa ctgatgatgg agggtatcta
     2221 attcttggat atactctttc gtatgggaca aatggggaaa tctaccttgt aaaaaccaac
     2281 gctacaggag atatgttatg gacaaaaaca tttggcggaa accatataga cagaggacat
     2341 tcgcttgaac ccacttctga tggaggttat atcctctcag gatattctgg gtcttacagc
     2401 tcaggaaaca tcgatcttta ccttctcaag attaatgacc tgggggagga ggaatggcat
     2461 aagataatgg gcggcaaagg cactgaaaaa ggatatgatg ccaaacaaat agaagatgga
     2521 ttcataatcg caggctatac taattcaaca ggtgctggac tttatgatat gtggttgatt
     2581 aagacagata actttggaaa tgctttatgg aacaagactt ttggcggcaa gggctttgaa
     2641 atagcaaggt cacttatcat aactgaagat gggtatttgc tatcaggata tacttcatcc
     2701 tttagcggca actatgatgc ttattttgta aagactgata atcagggtaa tattgaatgg
     2761 aacaaaacat ttgatactgg gctggatgaa caaatctggg aaacaacata ctcagatgga
     2821 tactatacgg catgcggtaa ggcatcagaa gatgcatacc tagtcagttt ctatgctcag
     2881 cagatgaagg ttgatgcctt gaatgatgga tctacagacc gcaggttgtt tccttcttat
     2941 tcagcaaaaa taagcatatc aggtaaagaa tctttagtgc ccatatccat aagttcctac
     3001 aaaggctttg cagagataac aaacatcaat cttgcattta gctatcttaa tgcttataat
     3061 ctttcgctgc aaaatggatg gaaccttatt tcactgccgt taatgcaggt aaacaattcc
     3121 atatgccatc tttttaaaaa tttagcagta tttggatatg acggaacctg gcaatattat
     3181 tataatgaaa gcgccaataa tttcagggag cttgagccag caaaaggata ctggataaaa
     3241 tcatctgagc ctcaagagtt gtcaattgcc ggtgaggagt atgagaatga agtcgatatc
     3301 aagcttaaag ctggatggaa tcttataggg tatccttatc ttactgaggg aaatataaac
     3361 ggcaatgctt tttcttacaa caattcatgg catagaacaa atgttcttga gccttggaaa
     3421 gggtattgga tatatagcag gaatgatacc aatctttcga tagaataacc tttagagaag
     3481 aaaaatattt aaagagtttt tgtcgagaag gctattgcat gaacaaaaga gggcagatag
     3541 cagtatttgt gattattggg attgtggtac ttgttcttgt tgggcttgtg ctcgctgcca
     3601 aatcaagtat tgataaaaag gtaagtgaga gggagaccgc caaagcagga gagatagcct
     3661 ttgaggtagg gccgataaag gcatatgcac agggctgcct gaagatggct ggtgagaacg
     3721 ggatttggat actgggcttg catggtggat atctgaatac atccgtagac aataggtata
     3781 aggaagaagg cacagcagca aatatggttt acctgggaac agacatcccc tactatcttg
     3841 gcgctggagg cagtgttttg gaactatccc aaatcgagca aaaacttgca agatatgtca
     3901 tagtcaaatt tgacgaatgc atggcgctca ataactttga tgaagatgga ttttctcttg
     3961 aacggcctat tattgattat gcaggcatcg gctttaattt tagtgcaatc aatgtaaaaa
     4021 gcaatgtcag catcaatcaa gataatgtcg ttgtcaatgt tgcctatcct atgaaagcca
     4081 aaaaaaagga gtttgaggca cggctgagtg aatttagagt tgacttacct atcagggtgg
     4141 gcatgatata cagcctcatt gccgacaata tccttactaa catatccaac tcatgggcga
     4201 atggccagct gtttgatgct tctgcaataa actgcaatga atatgatata tcaaataaga
     4261 ctaatatata tagcataagc atgggaaatg atggcatagt acgcattatc gattactatc
     4321 catactatca taaatatacc aaatcattta tattccagtt tgcagtgaat aacaacccgc
     4381 aagctgtcag tgaaggaaaa tgcacagacc attaaataat atctatatcg gaatactgct
     4441 tatcattata gcctcttaca gctatgcagt agaagatgat gctgataagg acggcatacc
     4501 tgatgcgaca gataaatgtc ttgcaggcag catccttcct gtagacaatt atggatgtga
     4561 ttgcagccaa aagagggcat gcagcggtga taaatggtgc tgtcctgctg gcgcagcatg
     4621 tatagatcat gagtcgagcg caagatgcgc agaggactca gacaaagaca atgttcttga
     4681 tataggtgac agatgccttg gaacaaagca aggctttgct actgatgaat atgggtgctc
     4741 ctgtgagcag aaagaatgcg atgacaacaa tccttgcaca gacgaccaat gttcacctgc
     4801 cagcgtccag tgcacctttg ctgttgacaa caccaatact tgcggacaga acaatgcctg
     4861 tcaagaggga gtttgcatct atcagggata tgaggagtat tactattatg tctatgatgc
     4921 tgatcaggaa aaattatcaa tatattacaa gcattcagtg catgtcatcg atgtagtgtt
     4981 tagcgagcca ctgctagaag acattatcgt aatcgactcg acaagcgagg tcgttgatta
     5041 tcaactgcag gatgaccgta aaaaactgac agtcactgtg caagggaagg atggaacaga
     5101 aggccaaaca tcgataaaaa caaaacaaga gccaaagtca gtgtccatag attccaggca
     5161 gattccaaag ataaatgaaa agctttctcc aaagagtcca acctggcttt atatcatctc
     5221 cattgccatg atgtttggta taatgcttat cgttgtattc tctttaagaa gacacgaaga
     5281 cgttgttatg ggaactataa aaaaagaaga ggacaagata gaaagagagg tcagccttga
     5341 ggcagagctt cagcttaaga tgtatataat gaccaacctt aaaagggggt accaggctct
     5401 gcaaataagg aatgaactga taagagatgg atggaagcct catgtggtag accgggtgtt
     5461 tcagagcatg agcggaagat tttataagta gctggaatat ctcgttctta tgagcataat
     5521 tgccattaca gggtctgtgt gcagtggaaa gaccacccta agcaagcttc ttgctagaaa
     5581 gcttggattc agatatatat ctggaagcga ggtagtcgaa aagtacaagc tttctttggg
     5641 ctatgacaga aaaaggaaat gcaagataat cgatgagaaa cagttttcga aagctgtctt
     5701 aaaagagatt gacggaaaag atgctgttgt ggattctcat atgagccatt atctgcctgc
     5761 aaagcatgtt gatttatgca tagttacaaa atgcgatatc aaagagatga aaaaaaggct
     5821 tagcaaaaga aactatagcc agttaaagat aaaggagaat atcgaatgtg agatatttga
     5881 tgtttgcact agcgaggcaa gggaatttgg gcataaagtc ctagttatat ccacaacgaa
     5941 aggtataaat attgaagaaa ttcttggcag gctgacaaaa aatggaatta aaccctcaag
     6001 caatagaact taacaatgca ataaaggcgg cgaacacaaa tgttaatgag atgctgtcag
     6061 agaaaggcaa agctatcttt tttccaaaaa agggaatact tggacaaggg gctgaggcaa
     6121 agggaaagaa gataaatgcg acgataggca tagcgcttga agaagacggc agccctatga
     6181 ggcttaagtc catatcaaag agggtcaagc ttgagccagg tgatgttttt ccttatgctc
     6241 ctagctttgg caaaaaagag cttagagaca aatggaagga gatgctttac aagaaaaatc
     6301 cagggcttct tgggaaagag ttaagcctgc caatcgtcac caatgcactt actcatggcc
     6361 ttagcatggt agggtatatg tttatcgatg ctggcgataa ggtgatactc cctgaccagt
     6421 attgggggaa ctacaggctg atactttcta atgcatacgg ggctcttctt cagacttttc
     6481 aaatgttttc tgggagcgga tttaacctag atgggcttag ggaaagcttg caaggagaag
     6541 gaaagaaaat actgctctta aattttccta acaatcccac aggatacact cccactatcg
     6601 cagaggcatc tgcgataaga gagataatcc ttgatgcagc aaaacagggc cagaagataa
     6661 tagccatagt tgatgatgct tattttgggc ttgtatttga agagggaatc ctaaaggaat
     6721 cgatattctc aatgctttgc ggcctgcatg aaaatgtcct tgcaataaag cttgatggag
     6781 ctaccaagga agattatgcc tgggggctta gggtaggatt tatcacttat ggaacattag
     6841 atggaaacag gcaattatac gaggctttgg aaaataagac aacaggtgct gtaaggggaa
     6901 gcatatccaa ctgctcgcat cttggccaat cgcttgtata ccatgctttc agcgacccaa
     6961 gctactggaa tgagaaggca aataagttta agattctcaa tagaaggtac aaagaagtaa
     7021 aaaagacgct taagaataaa tcatacgggg aagagttcag cccacttcct tataactcgg
     7081 gctattttat gtgcatccag cttaagaaaa aagacggaga gcatgtcaga aaaatactct
     7141 tggacaaata tgatacaggg gtaatctcac ttggcaatat cattaggata gcattctctt
     7201 ctgtaaggac agaagacata aaacaattgt ttgataacat ctataatgct tgcagggaat
     7261 aaaatggcaa aatcaaactt gagagacaaa ttgcactata tctttgggca tatcatggct
     7321 atagtcgcct tggtaatagt agttatagta tttggaggag cttattattt tatcatagct
     7381 ccaaaccttg tctcaaaacc ctcaatcgag aagcctgctt tttctgagaa tgccatcgaa
     7441 gagatgagcg agggaaaaca ggcaataagc cctgagcata tcaactatct tgtaaacgag
     7501 ctagatggct ataaactaaa gaatacctgg ggagacaaac ttcccataat cgaatttttg
     7561 ttcacagata caaacgtcag gtattactca tatgtcaaag accatgttcc gataaccaaa
     7621 gaaggaaatg caaagggaaa tgatatactt atcaaaggca gccagcaggt ggcttacgag
     7681 ataattgagt caggcaatat cccccaggca acaaaacagg ctgtagattc aggcaagctt
     7741 tctgtagagc ttgtgtctga catgaagacc cttgctacaa aaggctatct ttctatatat
     7801 gacaacgtaa gatgaagata aggataaggt gcggccaaat agataaagaa gctcagcttg
     7861 atgattcatc taccgcaaaa accgcctatg ataacctacc tatcaagggc agcatccata
     7921 gatggggaga tgagatatat tttgatgtcc ctttccatat agggcttgag gtggatgcaa
     7981 gggaggttgt ttttaaagga gagatcggtt tttggcctga gggagactgt ttttgcatat
     8041 tctttggcaa gacgcctatg tctgaagaga atgagataag ggctgcatct aaagtaaatg
     8101 tatttggcaa gataatagga gatagtactg tgtttaaggg tgctcttgaa gggcaaagca
     8161 tactggtaga gaaaatctag ttgttatcct cctaacataa tctatttaaa tggtcattga
     8221 atcttttttc ctatggaaac tgataatgag agcttgagaa agctgaaatc acttggaaat
     8281 aagcatgttg agtcaatagt acagcaatat attgaaatct gcagacctga tgaggttatc
     8341 gtctttgata attcagaaga tgatatactc agagcaagaa agcttgcgct taatggtgaa
     8401 gaaagggcgc taaggacaaa aggacatact gtgcatttcg acggacctaa agaccaggga
     8461 agggatctta tcaatacatg cgtacttgtt ccaaaagggg taaagctaag cgaagcgata
     8521 aaaactatcg atagggaaga agggataaaa gaggtaatgg gaatacttga gggcataatg
     8581 aggggcaaga ccatgtttgt caaattctat tgccttggac caaacaattc caaattcagc
     8641 atacctgctc ttcaaatcac tgattcggcc tatgtgatcc atagcgaaga tatactttac
     8701 aggcaagggt atgatgagtt taaggcattg aaagggtcaa agaatttttt tcattttatc
     8761 cattcagcag gcgagattga aaacaatgtc agcaagaata tcgataaaag aaggatatat
     8821 atggacctta ttgaagaaag ggtgtttact gtcaacaatc aatatgcagg aaacagcgtc
     8881 ggcttaaaga aacttgcact gcgtcttgca ataagcaagg caaacaagga agactggctt
     8941 tgcgaacata tgtttgtgat gggagcaagg cctgaaggca aaaacagagt gacttatttt
     9001 acaggtgcat ttcctactgc ctgcggcaag acttcgactg caatgatccc tggacagacc
     9061 ataataggag atgacatcgc ttatatccgg cctgatgaag aaggtaccgc cagggctgtc
     9121 aatgtcgagc agggtatatt tggcataatc tcagatatca atccaaagga tgatcctttg
     9181 atatacaagg cgctaacgac tccgagagag acaatatttt caaatatttt tgtgaaggat
     9241 gatatgccct attggaacgg catgggaaag gagattcctt ctgaaggcgt caatttttcc
     9301 ggcgaatggt ccaaaggaaa gaaggacagc aaagggaatg atatccctcc tgcacatcct
     9361 aatgcacgct atactatcgg gataaacgag cttgacaacg ctgattccaa ggcagattct
     9421 cctatcggtg ttcctgtatc tggttttata tacggcggaa gggattctga tactacagtt
     9481 cctgtacttc agtcacttag ttggcagcat ggagtcttta ttggcgctgc acttgagtct
     9541 gagacggtta caggaacaac tgacaaggca ggcgtacgga agttcaatcc gatgtcaaac
     9601 atcgagtttt taactgtgcc tctaggagtt tatatcaaga accacctaac gtttggagaa
     9661 gagcttgaca agcctcctct agtctttgcc accaattact tcctgcagaa agatggaaag
     9721 tatgtcaacg gcaagctaga caagaaggta tggatactct ggatggaagg aagagtgcat
     9781 aatgaatatg aggcgataga gacccctatt ggacttttac ccaagtataa tgatctgaaa
     9841 aagctgttta gggctgtgtt ttccaaggat tactctcgtg aggagtatga gtaccagttc
     9901 tctgtgcgta ttaataagtt gcttgagaaa ctcgacagga tagagctgat atacaaggtc
     9961 gagcaggata tacctgatat cttccacgag cacttaaacc agcaaaggca aaggctaaaa
    10021 tcagcaaggg aaaagtttgg aaaggatgtc ataagcccgt ttgagtttga ataagtttaa
    10081 atacagtttt ctatttttta agattatgaa agctggccta gcatctattg agatacatta
    10141 tctggttgag gaattgcagt ttcttatagg gggcagagtg gacaacatct acaacccttc
    10201 aaatgaagag ctgcttatcc aattctttgt gtctggaaag agcaaacaga tgcttaggat
    10261 aatttctggc aaattcatgt atctggcctc tgaaaaagag gaagcagggt cgccttcggg
    10321 cttttgcatg ctgcttagaa aacatcttgg aaatgcaaag cttaggagta taaggcagat
    10381 tgagcctgaa aggatagtgg aaatgctgtt tgaaaaggat gagataaaga aggtcatcgt
    10441 ggagctcttt ggcaagggaa atatcctaat atgcgataaa gaagataaca taatctctgc
    10501 gcttgtatac cacaagttca aggacagaga gataaaacaa ggactgaaat attcctatcc
    10561 gcagatgggt ataaacctat ttgacatagc tgagaaagag atagagaaag tatttgcaga
    10621 gtcaggcaac acccttgtaa aaactcttgc cacctgcctt ggccttggcg gcacttattc
    10681 tgaggagata tgcaatatct ctggggtgga taagaacaag aaggcatcag aggcaaaaca
    10741 agagataaac ccaataaaca aggcattgaa aagagtcata aagggagaga aagaggctgc
    10801 aataatattt aaggacaaag aggtcaagga tatagtccca atcaagctca atatctttga
    10861 gggttatgaa caaaaggtgt ttaaatccta taatgaagcg cttgaccatt actttctttc
    10921 tgtcaaggaa gtgccaaaaa atccccagat agagaagctt aagagaataa tcgtcaacca
    10981 ggaagaaaaa atcaagagcc ttgagcatga agagataact gaaaggcaga agggagatta
    11041 tatctaccaa aactatgcct caataggaag aatagtcgaa gagatcaagg aagtggtaaa
    11101 aaaagaagga tggggaggcc tagaaaaaaa ggcagagggg cataagatag taaaagcggt
    11161 gaaggccaaa gacaagactg tcgagataga attatagttc ttagaaaagt ttaaataaaa
    11221 agggacaaaa gaaaaagatt atggctttag gcagagaact tgacaggtat atatcaggca
    11281 aaaggaagag agaaagtttt tcttccttca aagagagggt gctgaacaaa aaatcagctc
    11341 tttctaacaa gatgtttgac cgtctgggag ctattactca gggaggagaa gcagcaaagc
    11401 cagatgttcc tgaagatgag ggatggaaag aggttctttt agagggctct gctgagccag
    11461 agacggtcat cgacgagcca gtgataaaac aagcacagcc ggcagtaaac aaagagccag
    11521 ttcaaaggcc tacaacccaa gaaagaaata tcccacatac acaaccccct gccagagaga
    11581 caaacgaaga gactgctgag gagataaggg aatcaaacaa aggcaggctt gaacaactta
    11641 ggatagcaag aaagcaagaa agaatgggcg ccctaagagg gaaggttttt aacatacttt
    11701 tcaagaagaa agagaagaag gaaaaagata tggctgaaca ggtcaggaca gagataaggc
    11761 tgcaggcaaa aaaggaagag gcaagcaaaa gagcgataat ccccgagaag cccaagatag
    11821 tcgaacataa aaaatcattc ttctcaaact tcatagagat aaagaccgcc gaggagatag
    11881 ctgaggatga caaggcagcg gcaatgcaag aacaggcagt agccaatgtg gaagagataa
    11941 acaacctact ttcagggcag cctggcgaga taaggacaaa ctgtcctgcg cagaatcctc
    12001 ttgagtttcc tgaactcttc cctgctagga aggaaagcgc gatagaggta ggaaaggtca
    12061 tatcccttga taatgactat aagataaaag tcatcaggaa taactaaaaa acgttatatt
    12121 tttaaagaca gataagtttc tagcttgtta gacggccata ggggcttggc tccactggtt
    12181 cccatttcga acaccaccgt taagcaagcc accgttcctg agagtactgc actacgtggg
    12241 aaatcgggaa agctgtcgcc cttttttact atgcataaat aattaatcca acacttcata
    12301 atctttttaa actattatcc cttctttttt caatggtgat acaatgaaaa tagcagtgat
    12361 ggggacaggt tatgttggac ttaccgttgg gacatgcctt gcagaacttg gcaatgaagt
    12421 tatctgtgtc gatgtcgatg aaaaaaagat caaaagccta aatgaaggca agattccaat
    12481 atatgagcct ggacttgagg atatgctcaa aagaaatttc aaagagaaaa ggatactatt
    12541 ctctacagac agcaaaactg ccataaccag ttcctctgtc atatttatcg cagtgggaac
    12601 acctcctgga aaagaccaca cagctgatct gtcttttgta aagcaggtgg caaaaaatat
    12661 aggaagccat atgaacgcgt ataaagtcat agtcaacaag agcactgtcc ctgtggggac
    12721 agcacagctt gtcagccaga tagtcaagga aagccaaagg caggctatgg actttgatgt
    12781 cgtgtcaaac cctgagttcc tcagggaggg agaggcgata aacgatttta tgatgcctga
    12841 cagaatagtg ataggggctg acagcgagaa ggcaaggaag gtcatggaga acatctatat
    12901 aggaatagcc aggacaagca agcctataat gtttacagac acaaaaagtg cagagataat
    12961 aaagtatgca agcaacgcta tgcttgctac aaggataagc ttcatgaacg agatagcaag
    13021 gctttgcgaa aaggcaggcg gcgatgtaaa ggaggtcgcc aagggaatgg gctttgattc
    13081 aaggataggg ccaagatttc ttcaagcagg tgtaggttac ggcgggagct gctttcctaa
    13141 agatgtaaaa gcgcttgaac agacgatgaa cagttataac atcaagagcc agatccttaa
    13201 agcagtagag caggtaaacg aggagcaaaa aaaatctgtc cttccaaaaa taaagaaaat
    13261 tttgcctgag cttaaaggga agactattgc agtatggggc cttgcattca agccaaagac
    13321 agacgatatg agagaggcac catcactggt aataatagac cagctgcagg cagaaggagc
    13381 aaggataaag gcgtttgacc ctgaagcaga agctacggca aaacttgtcc ttaaagatgt
    13441 cgaatatgca ggccatcctt tggaagccat agagggagct gacgccctta tcatagttac
    13501 agaatggaat gaatttagaa accttgacaa ggaaaaaatg atacagctca tgaagcatcc
    13561 aaacataata gacggcagaa atgtctatga gccaaaagag atgaatgcct taggatttaa
    13621 ttatatgggt gtgggcagag ggacagaatg aaagcgataa tccttgcagc gggatatgca
    13681 acaaggcttt atcctcttac caaagataag gcaaagcccc ttctttctgt cgccggaaag
    13741 ccaatcatag agcatataat ctccaaggtc gaacaggtag atgaggtaag cgagatattc
    13801 atcgtaacaa acgacaaatt cgcagagcaa ttcgagaagt gggctttagg ctttagatgc
    13861 agcaagaaaa taaaaatagt caatgacatg acgaagacaa atgaagatag gcttgggtct
    13921 cttggggatg tcgatttcgt cctggaacat gagaaagtaa aagagagcat actggtgata
    13981 gcaggagaca accttttcca cttttcccta agagaatttg tgcagagcca taagaagcac
    14041 aacaaaagcg ctgtcgcgtt atatgacgta aatgatatgg agcttgccaa acaatacggc
    14101 atcgtaggtg taaacaaaga gaacaagatg atagagttcg cagaaaagcc ggcacagcca
    14161 aagtccacac ttgcatctac cggagtatat atatacccgc cccatgtact gccgatgctt
    14221 gacgattttg tgaaaaaatt taaaaacagc gacaaagcag gtaacttcct cgaatggctg
    14281 cataaggaag agcatgtata ctgctatatc acaaaggata aatggtttga tataggcaca
    14341 catgaacaac tagccaaagc acaggaggaa tttaaggaat aaaaatccca accgccccaa
    14401 ggggcagggt attcttaaag ttaagatacc ctgcaaagaa aggcaaaaat gaatgagagt
    14461 aacccagtcg ggctagtggc ggcggtatct taacaaatga agacaattgt tgtcagcgga
    14521 ggagcaggat ttatcggttc acatctctgc gatagcctta tcaattccaa taagataata
    14581 tgtgtcgaca acctttttac aggaagcaaa gcaaacataa gccatctcct caagaaccct
    14641 aactttacct ttatcaagca tgataccata gatcctttgt ttctagacga cctcaaaata
    14701 gaccaaattt acaacctggc atgccctgca tcacctgtcc attaccaatt caatgctata
    14761 aggacaataa aagcaaatgt gcttgggacg acaaatatgc ttggccttgc aaaaaaacac
    14821 aaagcaagga tacttcaggc atcaacctct gaggtctatg gagatccgct tgagcatccg
    14881 cagaaggaga gttattgggg caatgtcaac tgcacaggaa ttagagcatg ctatgacgaa
    14941 gggaaaagat gcgctgagac actttgtttt gactatcacc ggcaaaacaa ggtagacata
    15001 agggtagtca ggatattcaa cacttacggg ccaaggatgg caaaggatga tggcagggtg
    15061 gtatcaaact ttatcaccaa ggcactgcag ggaaaggata ttacggtata cggcgatgga
    15121 agccagacaa ggagtttttg ctacatcagt gatcttatcg atggattaat aaagatgatg
    15181 aacaatgatg agtttgtggg gccagtcaac cttgggaatc ctgaagagtt tacaatactg
    15241 gagcttgcca aaaaaatcat tgagctgtcg gactctaaat caaagataat gtttgagaag
    15301 ctgcctgagg atgatccccg gctgagaagg cctgatatta gtaaggtaac tgcaagaatc
    15361 ggatggcgac ctaaggtacg attagacaaa ggcttaacaa ggacaatcga tttctttaaa
    15421 aaatgagaat ttgcatacta agtacaacct acccaaggta caaagaagat gcttgggtgc
    15481 cgtttaccca ttcattatca aaagagctgg caaaaaaaca tcaagttaca gtcgtaacct
    15541 cttcggatat agcagcaaag gattatgaaa agagggataa agtaaagata atccgcttca
    15601 tctatttctt acctaaaaaa tggcagatgt tgacttacag aggaggcatg cttgaaagct
    15661 ttaactcaag tttgcttgca aaagtgcaag ctcctttatt ccttctctct tttttcttaa
    15721 aggcgctcaa acacagcagg agatgcgata tcatccatgc ctattggtcc ttatctggct
    15781 tgattgctgt gctagtaagc aagatacgga agaaacctat agtgttgaca cttcatgggt
    15841 cggatataag gtcactgccc agatggctga atagatatgt ggtcaataat gttgatgctg
    15901 tcgtgagcgc tcatcatgag ctccttgata tcgctgaaaa actcggtcgt aaagaggaat
    15961 tatacaacat caaaaatccg atcgacagca aattctattc caagataaag acagatggtg
    16021 tgaaaaagga attcggtctt aaggttgaat atgttgtgag ttttatcgga cggctagagc
    16081 cgatgaagga tccgctcact tttatcaagg ctataccttt tgtgctagcg cagatgaacg
    16141 ggattaaatt ttttcttgtt ggcgatgggc accttatgca ggaggctgtt cgcctggcgg
    16201 aaaaacttaa ggttaaggag catcttttca taatagggca gagacatgac acgcatgaaa
    16261 tattgaaagc cactgatatt tttatcgcga ttagtccgct ggaaaactgt tttagcacaa
    16321 caattatcga atcaatgagc ttgaaagtcc cgtgcatcat caccgacgca ggatatacaa
    16381 aaaaattctt cactcacagg aaagactgtt atctggttaa aagcagagat gaaaaggaac
    16441 ttgcaaatgc tatcatcacc cttttacttg agcaggacct aagaagaaag attgcgctca
    16501 atggccttaa atttcttgac aacaataaat tcaacaacag ccagataatt aaaggatacc
    16561 aagctgtcta caagtcagtg tttacttgac cgatactgag tagaaatagg ttgcggtaag
    16621 attgaactta tcaaacgcat ccagataggt caatgtcctt agaattggcc aggcaagcac
    16681 cctaattaaa aagcccatgc cgaaaagtgt gtccaaagcg tatgtgccgg taaagaaaca
    16741 attgcctagt gaggttatat accctccgca tggctttact tctgtatgct taaaatcctt
    16801 gagtaggtaa tataatccat ctttagtaaa tctatagtaa tcccttggtg aagcatgaat
    16861 cctataatag aaggggacaa gcgtgaaaag aatccctcct tttttgagta cccttcttat
    16921 ctccctaact gtctggaaag ggtccttgat atgctcaaag atcgcttttg caattatgat
    16981 gtctgtagaa ttatccttaa ggggaaggca taatgcatct cccactatat ctggccttgc
    17041 tgaatcatct atatctaatc tgaagtagtt tttgtatttg cgcatatcaa aagagccgac
    17101 atttcctcca atgtctagta tcaggctttc tttgctgcat atcttattaa gcagtgcctt
    17161 atcgtccaga tttggataga tggggcatat gaatgttgat ttgtacagtt ttccaaggaa
    17221 agaattggat gaatgcagcc tttcataaat cctgcctgaa agggtatttc tccaccttac
    17281 cttctggtaa agctctcctt tatatttttg gcttttcata gaactcagcg atttttgtgg
    17341 tgacaaatgt gatttcctct ttcgtcaagc caggaaacat cggtagtgag attatctcat
    17401 ttacgacttt gtttgttatt ggaaaatcat acggcagata gcctagggat gagtaagctt
    17461 tttgcatgtg gataggtgtc ttgtaatgga tgtttgtaaa gatattactt gcctttaaaa
    17521 aatcctgcag catgtctctt tcttttaccc taatctgata tacatggtaa acatgctttg
    17581 aatattcttt ctcgattgga attatcactt tcttgagcaa ttcgttatac ctctgtgcat
    17641 tttttcttct ttgttcattc cagctttcaa ggtactttag cttgactcgc aagacagcac
    17701 cttgcaatcc ttccatccta taattatagc ccaccatctg atggttgtac ttagggtatt
    17761 ctccatggct cctaaggagc tttaccttct cagcgacccc ctcatcatct gttactactg
    17821 ctcctgcttc tccatatgcg cccaggttct ttcctggata gaacgaaaag cagccggtag
    17881 cgctgactgg aacttttctg cctttgtatt cagctccatg ggcttggcag cagtcctcta
    17941 tgattattag gttgtgctct tgtgcgattt ttactatctg atccatatca catggctgcc
    18001 cataaaggtg tactgggatg attgcctttg tatttccggt tattgctttc tgaatcaggt
    18061 tgacatcgat gttgtatgaa tcctcttcaa tgtccacaaa cacaggggtt gcccctgcaa
    18121 gtgatattgc ctcggcagtt gcaaaaaaag aattaggtac agtaatgacc tcatctccct
    18181 tacctatgcc atatgcaagc aaagcaaggt gcagagcagc agtccctgag tttactccta
    18241 aggctaactt ttttccgcaa tagcttgcaa actccttttc aaaagcttca acttcctttc
    18301 ctaagacaaa atcagtgtta tccaccacat gcttgattgc agcatctatc tcttccttta
    18361 ttgaagcata ttgccttttt agatcgacga aatccacttt catctgtcag tcactttgcc
    18421 gattatttta gctggatttc ctgcaactat tacgttgtcc ggtatgtctc ttgtgacaac
    18481 cgctcctgcc cctacaatcg agttctttcc gatggtcacg ccgcataaga tagtagcatt
    18541 tgctccgata gatgctccat ctttgacatg agtgctctct atcttccagt ctgtcccttc
    18601 ttcttttaga ttgccttctt tatttgttgc cctgggtatc ttatcattga caaagcaggc
    18661 atgaggacct atgaacactt catctcctat agttatcccc tcaggtatga atgcgaatgc
    18721 ttggatcttg caattttttc ctatcttgac atctttccgg atctcgacaa aagagcctat
    18781 cttggtgtta tctcctatct cacacccata tagattgacg aaattgtaag ctttggtgtt
    18841 ctttcccaac ttcacatcat ccctgatcaa ctgtgtcatg ctatctgcac ctctgttcct
    18901 ttgttcttta aagaaagctg ggctgcttcg atgactttga ctacctgaag cccagaattt
    18961 ccatcgctcc tcggttgctt gcctgacttt atgcaatcga caaaatgctc agtcatgctt
    19021 cttaatggtt cctttaggtc gaccttgggg atattgatat taccatctcg atacatgtac
    19081 tggaattcct tatagctgtc ccagtgtttg ggttttgcct tatctatcgt ttcaaaagtg
    19141 actcccttat catacacctt tatcttttcg ctcagctcga tatcatcata gacaagcatt
    19201 ttcttgcttc ccacaagagt ggttctcctt atcttgcatg gatcaagcca gctcacatgc
    19261 aggtttgcca tggtcttgct cttgaatttc aacgttatgt atgcgctatc ttcaacctta
    19321 ggctggatat atgattcagc gatagccctg acgcttgatg gcatacctcc aagcatgtag
    19381 atgagtacag agatgtcatg gggagccagg tcccagatga cgtttatatc ctgctggaaa
    19441 agtcctaggt ttactcttac catatcgaca gtatagatct cgcctagatc tccgttgtca
    19501 ataagctctt taattttact cacaggtccg ctgtattcaa aggtatggtc taccatcagg
    19561 accttgttct tttccttggc aagatcgatt aatattcttg cttcatcgct tgttgcagtg
    19621 attggctttt ctataaggac gtgttttcct ttttcgagcg cttccttagc cagcttgaaa
    19681 tgggtagata caggagtgac gatcgctata gcatcaatct catcgtcgtt tagaagttcc
    19741 ttgtgatcag tcattgtttt tatctttgga aaagccttgc ttatgtgctt gagcctgtcc
    19801 tgagatagat ctgcacaacc tataacacta cagtcttcaa tctgagagaa atttctgatt
    19861 agatttggcc cccaatatcc gcaaccgatt acccccacat tgaccatttt tcagattaag
    19921 gcattggcag attatatata aacgtttcta tatgtgtccg ataaggtttt tcccaccagg
    19981 acagtatctt tatcaaacag tccttatatt accgatatga aggaaacatt taaatactgg
    20041 cttatttacc gatatatcat gcgaggagag gcaaaatcaa atatcatcaa taccttactg
    20101 agaaataagc atgagcagtt aagcaagtat aagattgcaa agcttgcagg ctgctcgtac
    20161 tcatgggtgc atgagtatct ggatgagctt gaggcaaaaa ggatattgaa agggacaaag
    20221 gtgatcgacg aagctgcatt gaaaggtcag cttgctttag gcgaaaaaaa agaaagggca
    20281 aacatgccga tattgataat ccttgcagca ggtgtgggaa gcagaagcct tccccttaca
    20341 aaggacatac ctaaatgctt gatgaaggtt ggtggcaaga cccttcttga aaggatgata
    20401 ggccagaact atgccagcag cctaacatca gttcttgtcg tggtcggcca taagaatgag
    20461 cttgttgaag aagagctggc aaggctgaag aagacatatc ggaaactcaa ggcaaagacg
    20521 ctgttcaatt cagattacct gaccacaaac aacatatatt ccctttggct tgcgagggaa
    20581 tctgcgaaaa atggattcat ccttgtggat ggagatgtca ttgtcgatga cgctataatc
    20641 aggaaggtga acgaaagcaa tatccctaat ctccttttga tcgacgagga taaagcacct
    20701 gaagaggaag atgtaaaggt tttgatggat tcttcaggca tttatggtat tggaaaacat
    20761 attgagctta ataaatcaaa cgggataagc gtcggaatcg ccaaattttc aagagaaggc
    20821 gcaaaggtgc tttttgatga ggtaaaaaag aagctggatg agggaaaaag gaacatattt
    20881 tatgaggaag cgtttgaggc gatgacctcc aagattagga ttgatacgat cactacaggc
    20941 tcagctcatt ggagagagat agatttttta agcgagtatg aggatgcaaa gaaggtgttt
    21001 agatatgatc agcgctgaag agatactaaa taaagacaag gaatatgtga taagggcatg
    21061 gtcatcaaat actgggccaa tcccgatagt tgagggcaaa ggagtgcatg tcacagatgc
    21121 atcgggtaaa gagtacatgg attttgcttc tggatatttt gtcaatgtct gcgggcattc
    21181 ccatcctgat ataatcaagg cagtaagtga tcaggtgagc aggatcagcc aggtgtcgat
    21241 gaggcagacg actgttccat tggtattgct tgcagaaaaa cttgccaaga taactccagg
    21301 aaatctcaag aaaagctttt tttctacagg cggcgctgaa tctgtggaat ttgccatgaa
    21361 gatggcaaga tgctatacag gcaagcatga gataatcgcg cttaggaatg cttatcatgg
    21421 gctaagcctg gcgacattgt ctgcatcgac aaataccagc tacaggggaa cagcatacaa
    21481 tcctctgcct tcaggcatat cccatgcgcc caatgcttac tgctacaggt gcaaatacaa
    21541 tgatcatggt tgcgacttgc aatgtgcccg agagattgaa aaggtgataa aaggcaagtc
    21601 atttgaggtg gtcagcacag aaaatatagg caccctgttg atagagcctg tacagagcgt
    21661 tggaggtatc atccctcctg atggatggtt cgagcttgtc aaggagatat gcgagaagca
    21721 tcatatcttg cttatcgtag atgagataca gacaggcctc ggaaggacag gcaagatgtt
    21781 tgcatgtgag cattggggca ttaaacccga tataatgacc cttgccaaag gtatatctgg
    21841 tggggtagga agcatgggtg tgactatcac aaaggacgag gtcgtttcta atttctcatc
    21901 aggaacatgc agcacctctg caggcaatgc agtaagctgc gcaggagggc ttgccctgat
    21961 agagaccctt gagaaggaga aactgccgga aaatgccgca aggatgggtc agtatctcac
    22021 taaggttctt aaagagatgg acaatcctaa ttttggggat gtaaggttta agggcctcat
    22081 gggcggagtg gagatggtcg aagataggga ttcaaaagag ccgcttcaaa aaaaggcgat
    22141 gtcaaaaatt gtaggtaaac tccaggaaca aggagtgatc atcactgtct cagggccgct
    22201 tggcaatgtt tttaggatac agccggcact aaatttgaca aaagagcaag ctgataggtt
    22261 cgttgatatt ttcagcaata ctgtgaaagg tgtttgcaat tcagccaagc tttgaccaac
    22321 tgaaagatgc gctaaaacag gaagacagcc ttttttcaac ctatttttac aggaggattt
    22381 cactgaggat aacctatctt cttatcagga cacagataac tcccaatcag ataacatttt
    22441 tttcccttat cctgggattg gtcgcttcat ttttattttc cctgggcagc tattattatg
    22501 cgctggtagg tattctgttc atccacttgt cttttctgtt tgatcatgtt gacggagaga
    22561 ttgctagata taagaaactc aggtcagatt ttggcgcatg gttcgaccag gtctcagata
    22621 ggataaaaga atcgactgcc tttataggtt tggcattagg tatatacctt gcgacaggta
    22681 gcagccttcc ccttatcctt gggatgtttg cggtattcaa tcttttcatg ataggtcatc
    22741 ttagagggac cacctctcag ctaaagtttg agcacacatc agaattaaag atcacaagta
    22801 aggttcacat aggaagtgtt gatacaacta tatttctgat aaccctggca gtgatatttg
    22861 atctgcagtt atacctgttg atgttatatg caacttttgg ttgccttgca tggttctatc
    22921 agatttacac aagatataag agaagcacca aaaccagcag atcctaatga ttgatccgct
    22981 tgttcttgat catgtctgca agaaagccaa aaataagtat ctggattcct ataactataa
    23041 ataacatgct taaaatcgct gaaggtatat gccttatcgc accgcttgat aaaaagaggt
    23101 ataagagcca gattccgata agaaatcctg taccaaatag gcttcctccg ataaatgtga
    23161 agaacttaag aggctcataa tcgcggtata tcctaaggat ggtcaggcct gctttgatag
    23221 cgtatgagaa aggatggcca aaaagcctgc ttttatcctt tcttcttgca aagtcaacag
    23281 gtatctcctt taccttgaac ttgagtttga tcgcccttat tatctgctcc tgggtatatg
    23341 tgaagtttga ggtgatccca actttttttg ccacttcctg gctaaaagct cttaggccgg
    23401 tctgggcgtc tgttatcttt gtctttgtta tgccagagat gactgctgaa aaagccctgt
    23461 ttcctatctt gtttataaaa ggcatatact cgatgtttcc tttgaacctt gagccaagga
    23521 caagatcatt tcctttcttg acctcttcaa tcagtctggt gatatccctt gcatgatatt
    23581 gcccgtctgc atcgatatgc acgatgatct ccgcacccat cttcaggcat tgctcgatct
    23641 ctgtcttgaa tgtctcggca agtccgtaat tcttcggatg agcaaacaca gccgctccat
    23701 gattttttgc gacaagggag gtattatctg tgcttccatc gtctacaacc aatatcttat
    23761 gatcatatga caagtctttc atcactttgt ttatgtcatc cagaacctgg cctagcgtct
    23821 tttcttcatt gaacgcagga attgttatta ttatcatcaa tggctaaaat gagaaattat
    23881 ttaaatatat ttccatcact tttggcaata atgggctttt ttacgatttt actctttttt
    23941 gtttactcat ggggccttgg ctactcaatc acgttctttc ttagaaattc agataacttt
    24001 tttgaaagaa acataatgag ggttggaata gggttgggcg ttctccctat ccttgcggtt
    24061 atcttgaacc ttgtaaggat acctcttgac tggaagatta tgcttgtgtt aagcctcgcc
    24121 atcccggtaa tttatctgat caatcatttt aggaatatca agcttgcaaa tccattcaag
    24181 gttagcaaat caaacgtgta tatatttatc gttttgctca tgtttctggc attgtttggc
    24241 atgtatcata agggatcttt tgcatacccc tatcttgaag acggggatcc ctggctttat
    24301 gctgtcgcag ccaaatatat agctgtccat gatacctatt caaaacctgt cgatatgttt
    24361 ggctctgagt atgagatctc acattataca gagccctatc ctgctgctta taacatcctg
    24421 atgggtatcc tccatcagac atcgccctct ataaattgga cactgaaatt cttcaatgcc
    24481 cttatgctct cattatccct tgtttttttc tatttttttg tcaaaaggtt cagccagaac
    24541 aagaatttgg cgcttgcctc gacgtttgtt atgctggtca ttccgtgctt tcttgggcat
    24601 tttatctttt cccaggtgct cggagtcatg cttttctttc cagcgcttta ctgccttgcc
    24661 atgatagagg atgacaagaa gtgggctttt gtttcagcag cgcccattgc aagcatcctc
    24721 ttctctcagc tggtcactgc agcagtcttt gcgattattt ttgccgtgcc ctatttcatt
    24781 gtcatattat tcaaaaaaaa ggagctaaga aaatacttta tccttgcagg attgcttggt
    24841 gcagccctgt ccttatcata ttggggagag atggtataca aatttggtta tgaaggggtc
    24901 agatatcaaa acagcatggg tagccagacc cctttcaagg tagggggtac tttgggagat
    24961 atatacggtt gggatgattt catctacgga gcttattatg gtaactccct gtttagcatc
    25021 agatgggcaa atgcgatcga cagtccgatc gggcttggat ggtttttgtt tgccttgctg
    25081 ctaatctcct ttgtcttggt gataatccag gcaagaaaaa ttgtaggtga aaagccatgg
    25141 ctgcttatat ctacaatatg gcttatcctt acttacgtta atatacgcag tggcgctctt
    25201 cctgtaggat tcgttcccca cagatcctgg actttttttg caatccctgt ttcaatcatt
    25261 gctggttatg gaattatcgc aatcctcagc ctgctggagt caaaagagaa aaaagccctt
    25321 gcactggtag ttgtgatcac tttattgttg gtgacctcag gatacccaaa atactatgta
    25381 aatactgtgc cttggcccca gcataggctt tttcctgaca tgaatggaga gcttggcggt
    25441 tatttgtggc ttaatggtct gccggtggac acaatggtta caagcctttg caggacagat
    25501 gatgtcgtca taggctttga taagatgagc acagcccagt gggacaggag tcagatcgac
    25561 ttcaagaagc atttggtaaa ttatagctct gtagagatta gcgaacacct taaaaagaat
    25621 aagtaccaat ttgcagtcat tgacagccag tgtgttgaaa ggtttggcat caatgaaaca
    25681 aacacaaaac ttaatgagat ggtcggctca ggattattta tatccgtgct gccgcaaaat
    25741 ttcaatggga ttgtcatcct taaggttttt tgaatggctg gatgaactgt tttagtatgt
    25801 ctatttcttc gtttcctatg ccttttatcg atagcagtac taatgcatac gttgcgatcg
    25861 ctattggaag tatgataaat agattaagcc ctctgataat atagatgaca attcccatcg
    25921 ctatgcctgc gaccaaaggc tttataatca cttttttaag gccaactcga tatccgttcg
    25981 ctgaacaata atggtataag aataaaaaac ttatcacttg ggtgaggact gttgctatag
    26041 atgcaccgat tattgagtat ctagggatga ggataaagtt cagcaataga tttcctccgg
    26101 cagctgctgc tgcggtatag gtaaacagcc tctgcttgtc gatcgagttt agaaggtagc
    26161 ccataaggta atttacaaac agaagcacct ctgtccatat aagtatctgc agcgcaggca
    26221 ccgatgcaag aaagggttct ttgtagatga ggaggattat cttgtcagca agaacaactg
    26281 ctcctatgcc gatgggcagg ccgacgacga ataagtagta aaatgaccta tggtataaca
    26341 tctgaagttt gcctttttca tttagatgaa gatgggacat cgtgggaaat atcgctgcca
    26401 cagctgcaaa tgatatgaac gagaatgcat cgactatctt gtatgccgcg ctgtaaaggc
    26461 ctgctgtcgc ataatctttc atgctagtca gcatgacaac gtccatcttg taatagatgg
    26521 tcataaaaaa tacagtaaac cagaaaggaa gtgatctttt gacaaagaat tgccaaaatt
    26581 tagggttcat cttaaatctt atcttggcaa actttttaga taccacatac atggaaaaac
    26641 cgaggcttgc tgaataagat atcaccagca cgataaacaa tgatatcaag ccatatcctt
    26701 tcaatatcaa aatggatcct atgatgactg caatgaggcg ctcgattacg tttgctgctg
    26761 aataaaactc catcctttca aaagcagaga atacattctt gaatatcagc gaaaagctgt
    26821 caaggaagag tgtgatcgag gaaagcatga ctaggattat aacatctgtc gactctgcaa
    26881 gaaagaagat ggcgatcgtg gatgcgatca acggaaggct gccgataaaa agcttcagca
    26941 caaccaggtt catcaggtac tcctgggcct tttgcctatc ccttgacact tcccgggtca
    27001 taagggttgt cactcctaga tcagagaaga tgaaaaataa tcccgagaat gcaaaggcaa
    27061 acgaatattt gccaaggccg acatctccga ggtatcttgc aatcaagaca aatagcagga
    27121 aggaaagcac cctgcccagg gtgtctgcaa aaagcaggct cccgacgttc ttggctacaa
    27181 ttttggctgt tggcataaat gtagatatga agaaaacctt tataaattct ttgctatgat
    27241 taggaccgat gacactcggt aaggcaagga tcttttccat cgctgcagtt atcatagcgg
    27301 ctgttcttgc ctttctttac cttcaaaaaa atccttatga ttttaaggga ttgactttcc
    27361 aggtttttct ggttttatcc atcttctcct ggatatcagc gatcctaaac gggataaggg
    27421 tcaagatatt ctgcgacatc ttcaacatcg ggctttcctt taaggaatgg ttcggcctgt
    27481 cgcaggtcac ttccatggga aattatctaa ctccattcaa gggaggaacc tcgcttaggg
    27541 ctatctacct aaaaaagcat tatgactttc cgtataacca gttcatcgcg acgatggctt
    27601 cgacggcggt catttccttt gccgtctatg gattgcttgg tttgatatct cttatcttta
    27661 tttttgccgc aaccaatacc ttcaatagcc tgctttttgc gatattctct atctgcacgt
    27721 ttggttctct tgtgatcttc atctttcctc acagcatcat cacaaggttg tttacaaggg
    27781 gatttcttgc aggatacata gctaagattc tggaagggtg gtccataatc aagaataagc
    27841 cttcagggat aatcaagctg attgcgatcg aggttttgtt ttctgcggca tttgcattca
    27901 gcatattctt tgcgttccgt gcagtcggat ttcgcttgcc gttcatctac tgcatgattg
    27961 tggcagtcat atcaaatatc tcaataatat ttcagataac ccctgcaggg cttggcgtca
    28021 gggaagcagt gactgtgtgg gcctcatctc tttttggggc aggtatcgct ctcccgttga
    28081 acgttgctct acttgtcagg cttgttgctt ttatttgggt tgttcccttt gggatgatat
    28141 acggatttat gcttcttggt aaaaagaggc agtaatcagt gactttaaat tattgtttag
    28201 taaatttttt aagtaaatcc ctactatcca catcgatgca taatcttttc aacaggcaca
    28261 aggataggat aaaaatatat tccatactca tcgggatggt gctgttgtac atgcttgtga
    28321 agggatattc attttatgca tcagatcctt atgaatatgt tccaagcatc cagaaattta
    28381 ttgataatga ttatctaaag aatgattggt tcctgaacac gcagactggt tttaatgtca
    28441 ggacatactt ttattacttg atatcttttt tcctgccagt gctttcgatc aagagtactt
    28501 ttctactgct ttttatagtc aataccttct ttcttgtcct tgggatattc tatatatccc
    28561 agcttttgtt caaggacagg ctaagttcct atctatcctg ctttttggtc ctcttcagcg
    28621 tatggaagct cataggtgat tttgcgataa ttgccccttt cttcacacct tcccacatat
    28681 cgatcactct ttccatatgg gcgctttttt ttttcctcag gaaaaggtac aatatcatgt
    28741 tccttatgct tggcctgtcc actttcctgc acctgcttat cgggattagt gcccttatcc
    28801 ttttatctac agtgatactt gtcagggatc gtttttcaag cataaagata tttgccgtag
    28861 gctctgtgtt ctattcgcca gccctgctta gcatcatacc tttgctgtta atcgaatttt
    28921 ctcaaagaag cagcgcaagt gaggttatct tgacatttgc atggatcagg attccatggc
    28981 atcttatccc ttcccaattc agtctgatat catacatcag cttttctata ctcactttat
    29041 tatttttata ttcactaaga gatgccggaa aatcaaagat gaaccatgat atctatctca
    29101 tgttgatctg cgcagcgatc tactttttcc tgggttattt attggttgag gtgttcccaa
    29161 tagagctggc tatgaagctt caatttttca ggatgataat acttgtcagg ataatactgc
    29221 ttatctatat tgcaaaccac tttagcaaaa gttttgcgtc atccccatca gtgttgctgg
    29281 ccattacctt agcatcctta tttgaccata tattgattgt gatcgtccca ttttatctga
    29341 tatatgagtt atttatcaag aaaaaatttg caggctatac ctcaactatg tcatacgcct
    29401 gcctggcgag catagggctt actttctttg cttttatcct ttttggcaaa aatttcatct
    29461 catcagaatt tttccgaagg atattctcgg attacctgtg gatcttcttt attcttgctg
    29521 tatattatat ccatacactt agaaaaatga agatatacaa acttgtaatc ccttctgtgg
    29581 cgattgcttt ctgcattctt tctatagctg tcatcgccca gtttaagtca ctggagcctg
    29641 aaaaagataa catgtattca tggataagac aaaacactcc tgatgactct gtttttttga
    29701 ttcctcctta ccttgaagat ttcagggtaa aagcaatgcg tgccatagtg gtcgatttta
    29761 agtccatgcc ttttcaaagt gaccagatga tagaatggag aaacaggctt tctgatataa
    29821 ccagtaacat cgagttcaga tcagcaataa accgaaaaaa cgagcttaag gtaggctttg
    29881 aaagccttac caaagaggat attgcacgtc tgcaggataa gtatggtttc cagtatattc
    29941 tgattgaaaa accaatgaga ctacaacaga aaagtattta cgaaaacgca aaatatgccc
    30001 tttattctac ttcttgatcg ctcttagtat gagttcagac ccaaagacat acctcgagcc
    30061 caccacatta aaaccgtgct tacccagcaa ttttttaagg gattttcttg aataaggaga
    30121 tatatgctca tctgcatagg catttggaag gattttgcta tatatccatt cgatgatatg
    30181 ccaggatgcc ctgctatagt caggtgtgcc taagatcagt atacctcctc tttttaggac
    30241 tcttgtgatc tcaaggaaag gttgctctcc tttttgcaga tgctcgatca cttcagagca
    30301 gatgatgcaa tcaatcgaat cacttttcag aggtaggtta aatatggatg cctgcaaaag
    30361 attcttgttc ttgctcctta aaaacatcag tttcttgagc atgatatcga tcccgatcga
    30421 attatttagt tgttgtataa gcttgtttga accgcacccg atatctacga tcaacggttt
    30481 ttcagatggt ctaaacgatt gagctagaaa actcattatt actttatgcc ttgctctctg
    30541 ccaaagtctt tggggcagta ttttgctatt ataagccctg tcatcataat ctgctgcctc
    30601 tatcgaattt ctcatcttcc acatcttccc taaagtgatc aggtaagagc aggcaaattt
    30661 tgctaacttg gccttagact ttccaaattt tcttggcctg taatagaaag gttcctcttt
    30721 tataatccag ccattgttat aagcctgtac cactatctct tgaaggatat tgaagtctgt
    30781 cccttttagc tgcaggcttt cacccacgac ctctttctta tacatgcgat atccgctgga
    30841 aaggtcctta agtggcagcg acagtgctct cgcaaaaaat gcatttaaag atttgctgag
    30901 aaaagttctt gacaatgaca tcttggcttt gcctcccttg acatatcttg aggcgatgat
    30961 gatgtctgca tatgatcttt gcatccaaag attttttatg aaaacaggat catggctcat
    31021 atcagaatcc attgttagga catagtcccc ctttgccaag gcaaaacctt caagaagtgc
    31081 tcccccatat cccttgcttt tctgtatgat aacctttgca ccctcttttg ccgcttcttt
    31141 tacagtatta tctcttgaac ctccgtctac cacaataacc tcaaatttga tattcaatgc
    31201 ctgcaattgg ctttttaccc ggcggataag ttcgccaaca ttattttgct cattatatga
    31261 gggtaagatg accgagagcg attcagcgcc tttcaaattt ccataccccc ttgctttcac
    31321 ggaccatccg aaagccgctt gcttcccaaa atttaacagc atccttcctg tttgtctctg
    31381 agaaaagcgc atggcttggg atcagttttt ttccgatgcc cttaccccta tgtaaagcat
    31441 tcacgcataa cctatagatc gatctgtccc ttaggaagat aaatccagcc aagtcaccgt
    31501 ttgccttcgc gaccaggata tcccagccag gaacgacagg cgacaggacc acctgcttga
    31561 aaaatcccca cttggtaaat ctttccttgt tgcttaagtg atacatccta aggtaatcac
    31621 ttggtctggg gcaggatatt accactttct ccataggcta tccagagaac agggcatatt
    31681 ttaaattagt ttgtatttta taattgataa gttttataaa caattatgag tcacaatatc
    31741 ttccatgagc aggaaaattc ttgttacagg gggagccggt ttcattggtt cttttattgt
    31801 ggacaagctc atagagcaag gcaacgacgt caggatattt gatagtcttg agcctcaggt
    31861 ccatggtgat aagatgcctg actatcttga tcccaaggct gaattcataa gaggggacgt
    31921 aaatgatgca ggtgctttga aggatgctgt ttcagatatg gatgttgtct ttcatatggc
    31981 tgctttggtg ggagtagggc aaagcatgta tcagataaag cgttatgtcg aggcaaatac
    32041 ccttggcacc tcaaatctgc tggacatact tgttaactca gagcatgatg taaaaaagct
    32101 ggttgttgca agttccatga gtacctatgg agaaggcagc tatgaatgtg aaaactgtgg
    32161 cgaggtccat ccgcctttaa ggcctgagag ccagatgcag aagaatgact gggagctgca
    32221 ttgccctgta tgtgataagc attgcaagcc gatccccaca aaggaatcaa aaacccaaga
    32281 agttaattcg atttatgcga taacaaagaa agatcaggaa gacatggttc ttaatattgg
    32341 aaagacatat ggcattcctt ctgttgcttt gcgcttcttc aatgtttatg ggccaaggca
    32401 aagcctcagc aatccttaca caggtgtagc tgccatattc atgagcagga taaagaatgc
    32461 aaatccccct atcatctttg aagacggcaa ccaaagcagg gatttcatat ctgtgcatga
    32521 tatcgcagat gcaaacatcc ttgcgatgaa gcaccaaagt gctgactatg aggtgttcaa
    32581 cgtagggacc ggaaataaga taagcatcct cgaggtggct gagactatat cacgattgta
    32641 taaaaaaagt atcatgccta aacttgcata caagttcagg aaaggggatg taaggcattg
    32701 ttttgccgac atatcaaaga taagggataa gattggattt gatcccaagg taagctttag
    32761 cgcgggtatg ggggagctta tcgattgggc aggctcagtc gatgctgtcg atagggttga
    32821 tgatgctaca gacgagcttc aaaaaaaagg actgttaagc tgaaaattct accataagat
    32881 ttttatatta tccccggttg ctatgattcc atgaagatat ttataacagg gataaccgga
    32941 tttgttggaa gccatatggc tgaatttctg ctcagggaaa agcaccagat tttcggccta
    33001 aaaagatgga gaagccccct ggacaatatt aagggaatat tagataaggt tgagctagcc
    33061 gatggagacc tgagggatag caaaagcatt tatgatatta tcgctgagat taagccagac
    33121 cgcatctatc atctggctgc tcagtcctat gtccctacaa gttttaaggc gccaaatgac
    33181 acgatagaga caaacgtcat gggcaccata aacttgctcg aagctgtcag gcatgctaaa
    33241 attgatccaa ggattcatat atgctcatcc tcagaggtat atggccaggt gaccgaaggt
    33301 gagcttccga taaaagagac taatcctcta aggcctcaga gcccttatgc agtgagcaag
    33361 gttggagaag atcttatttc ctttcaatat ttcaagagct atggacttaa gtcagtgaga
    33421 agcagggcat ttacccatac cggccctagg cgtggaagcg tatttgcgct gtcatccttt
    33481 gcaaaacagg ttgcggagat tgagaaaggg catcaaaagc cagttatcca cgttggaaac
    33541 cttgagagta taaggacctt ccttgacatc agggacatgg taagagccta ctaccttctc
    33601 cttgaaagat gcgagccagg tgaagtctat aatatcggcg gcaatgcaac tatgactata
    33661 agggagatgc tcgaaaagct cctgactttt actaaggtta agccggaaat acgagttgaa
    33721 aaagaactcc taagaccttc agatgttact ctccaaatcc cggacgtgtc caaattcaag
    33781 aaggccactg gatgggaacc ggatattcct ttcgataaga cgataaagga tactctggac
    33841 tattggagag agaatgtcta aagaaagaaa catagtagtt attttgttgc ttttgatctt
    33901 ggggtttaat ttattctatg ctgcatacag aacagtttcg gtggatgaaa cagcatatat
    33961 catacagggt tatctgatac tgaccaaagg tatttttttc tccgatgata cgatgccttt
    34021 tgctaaggtt atcagtggac tacccttatt tttgctaaag ccaaacatac cttcaggcta
    34081 ttatgatcta tacctggata atgagttcat gaaacttggt gctgattttc tttacaaatc
    34141 taattccaat tcaggaatta tggtcttttt cgcaaggatc ttccctttgc tattaagttt
    34201 gcttcttggc ttttttgtct acagatgggc aagcgacctt tatggcacca aagcagggat
    34261 actgtccttg atattgtaca cattcagtcc taatattctt gctgaatccg cttcagccac
    34321 ctctgaccta gcagcagtat gctttatgtt cggctcaact tacttcttat ggagatatct
    34381 caagtttgag aaaaagacag atttgttggt tacatcctta ttgtttgggc ttgctcaagt
    34441 aacaaaattg actgctttgt tgttgatacc tgtctttgta ttatttctgg cttatcctgc
    34501 atttaaagca aggtcgtttg ataaggcaaa aaaagcggct atagcatttg cagttatctt
    34561 ggttgcctca tatataatga tcaatatggc attcctcttc cagggcacat ttactcctct
    34621 gggaaaaagc cttgctgatg acccggtaca ctactttgac aaggctaaat tcaattctga
    34681 atcgatatct cagggctttc ctgcatgggc aagaaacgca ggctcttatt atctcaataa
    34741 tgttccaact ttcatcccct atccttatct taagaatttt atgggagccg ttagaaaata
    34801 tgaaaatcca atcttcctgg gcggctattt aaatgggaaa ctacatgaaa acggagataa
    34861 ttatttttat tatcttgaga atttcctgct tcgtaccccc atcgccttga tattgcttat
    34921 catcctttcg attgctctgt caaaaaagta tacggatagt ataggtgata atataatgct
    34981 tttgataatc cctattgccc tcttcctgta tttcagttca gggacaatgt ggggcggcat
    35041 aagatatctt cttccgatat accctttcat gtttgtattt gtgagcaaac ttgctaattc
    35101 aagagaaaaa aagataattt tcccagttat agcgcttgct gcctggtttg tcatgggttc
    35161 gatccttgct tttcccagcc atcctgcata ttttaatgaa gcggctattg gtgaagggca
    35221 cttctttttg tcaggtccca gctttgacgt tggccaggac ttaaaagcac tcggcaacta
    35281 tgccaaggca aatagcttaa agggcttgaa acttgcctac tttggggagg gcagtccaga
    35341 ctattacgga ttaaattatt cgtacattgc atactatggg aatttgaaac aaagcgttga
    35401 aaattgcagc aggcaggaag ggcttgttgc cataagcatt acctaccttc atccaaccaa
    35461 acaattaaat aatcctgatt gtttcgattg gctttcagga tataaaccag ttaagaagat
    35521 aggaaatact atctttttat acgaggtcta agatgaaaat acccgtatgt gagccaaaac
    35581 tgtccggaaa tgagctaaac tatgttacag actgcataaa gacaggatgg atatcctctc
    35641 ttggagatta tatcgccaag tttgaggata agttcagatc gttcactggc tcaaagcatg
    35701 cgataacctg ttctaacggc actgctgctt tgcatcttgc tcttcaagcc ctgggcatag
    35761 gaagaggaga cgaggtgata atacctgatc tcactatgat agctgttcct tactcagtga
    35821 tatatacaaa tgcaagacct gtccttgtcg atgccgagat ggacacatgg aatatcgatc
    35881 ccgaaaagat tgaaagcaag ataacaggca agaccaaagc aatcatcgcc gtgcatacat
    35941 atgggcatcc tgtcgatatg gatccggtat tggaaatcgc aaagaagcat ggcatctatg
    36001 tcatcgaaga ctgtgctgag gctcacggag ctctgtataa gggaaaaaag gtggggtcaa
    36061 ttggagatat cggctgcttt agtttttatg caaacaagat cttgacaaca ggtgaaggag
    36121 gcatggttgt cactgatgat gcctctgttg aaaagaagat aaaactgata aagaatatgg
    36181 cttttgctga gccgagattc tcacactttg agattggatt taattatagg atgacaaatc
    36241 tgcaggcagc aattggcctt gcacagatgg aaaacgctga agagctgata gatgaaagga
    36301 tgaaggtctg caggatttat gattctttgt ttaaaaatat ccccgggata gtcagaccgc
    36361 cgcagaacgc atgggcaaag aatgtctact ggctttattc catgcttatt gatgattcgt
    36421 tcggcgcaag cagggacgag gttaaaagaa agcttatgga aaaagggatc gataccagga
    36481 acttttttac accgatgcat cggcaaccct tattcatgaa aaaagacgaa cggtttcctg
    36541 ccacaagtgg acttttccct gttgcagaca agctgtccag aacaggtatc cagcttcctt
    36601 caggggataa cttgagtaaa gagcaagtcc agacagtagt tgaggagatt ctatcactgg
    36661 caaaatgaaa agcatatcag tgatcctgcc gacatatgaa gaaaaagata atattaaaga
    36721 catgatacaa aggataaaaa gaagcgcagg taatctaaag gagataatcg ttgttgacga
    36781 taacagcccg gatctgacat ggaaactcgt gtcagagctg gcaaaaagag ataagaagat
    36841 aaagctgata agaaggatag atgagagggg aatagcctct gctataggca aaggcatcaa
    36901 agctgccact ggcgacatca tcatttggat ggattgtgac tcaacccagc ccccggaact
    36961 gattccaagg cttgttgaga ggcttgatca ctatggcctc gccattgctt caagatatgt
    37021 ggggattggt agcgataaaa ggaccatgac gcgcatagtc acaagcaagc tttttaacat
    37081 ttttgcgtct tttgtgcttt ggcttccgat aaaagataca gacagcggat ttattgctgt
    37141 aaacaggaaa gtgtttgatg cagtgccttt tccagcatat ggttatggcg aatattttgt
    37201 cgagttcatc tataagtgtc ataaaaaagg gataaggatg acagaggtag gctatgagtt
    37261 cagggacagg tcaaggggca ggtcaaagac cgcagatgat attcctactc tgctaagata
    37321 tggctggcaa tatgggatca aagttttgaa actgagggca ggattgatat gaaaataatt
    37381 gatgtacctt ttgagcatag tgatgagagg ggaattttca gggagctggc aagaggcgat
    37441 aaatggaagg agataaacgt cgccgagaga aaaaaaggga taaccagcgg caaccattat
    37501 cataaggtaa aggatgagct gttctatgtg tacaggggaa ggtgtcatct gcgcgtcctt
    37561 aacttcaaga ctggcaaaga ggataactat gattttaagg agaatgatat gtttgtttta
    37621 gagccatatg agatgcatga attttcatac tctgaagata ccctgtttgt gatacttctc
    37681 tcgcttgcct ttgagcaagg caattctgac acatacacct tgtgatagaa ctattttaaa
    37741 actattctac aactgggtga ttgccctaaa tcctcgccac attctcttca tatcctctat
    37801 gctccttata tttgcaagct gttttgcgat atatttcggc ctaagataat actccttgta
    37861 ggatttatcg aggaattttg catattcttg cttatctaca gaacttggag catagatgac
    37921 ctctgaatga tcaagttgtg agcaatattt ttcgatatca aacttatagc cctcttttag
    37981 caggttatga taaagctctg tgccgggtag tggggtgaat ttgttgaaat gtgcaaagtc
    38041 actattgatc tttttggcaa actcaagcgt ttgtctcatc gtttccattg tttccatggg
    38101 cgttccgcaa agatagtcta tcctcatgct aagtccgacc tttctgcata agtcagcagc
    38161 cctttggttg tcctgtatag ttgtcccttt cttaagcgag gcaagtacct gaggattgcc
    38221 gctttcaaat ccaaaaagga cctgccagca tcctgcattt ttcattgccg taagcatctc
    38281 ctcatccacc ctgttggctt ttgttaggca agtccagggg atatttatgc ctctctcctt
    38341 catcaatgca aagatggaat aaagcctctt tttgttcaag gtaaaagtgt cgtcaaaaaa
    38401 tttgatttcc tttatcttat atttgttgac gagctcttcg acctcagcaa cgacattttc
    38461 agggcttctt tccctgtatg tcgaaccgaa tattgagcgg tcgcagaatg tgcataggct
    38521 tgggcatccc cttgacgtca tcatgtgggt agctggaagt tttctataag aagctggagt
    38581 tggcctatat tttgaaagtg gaggcagaag atgcctggcg ggaaaaggaa gctcatccag
    38641 gttcttgata aattccctcc tttcgttgat gactacatgg ccgcctttct tgaaagcaag
    38701 gcctttgatc tgggcaagat tggcagagtt gttcttttca tgatcattaa caaagtccaa
    38761 aaaagtgtat tctccctctc cgatgattgc agcatcaaaa cattctgcct gcagtgtctg
    38821 tgcaggaagg gcagtgacat ggctcccacc caccacgacc aaggtatctg gaagctcttt
    38881 cttgatattc tgggcgattt tttttaccat gccgagcatt ggggttaaga tagacatccc
    38941 gacaacatcc ggcttctgcc ttattgcttc cttgataaca gtttcataag tcatatccag
    39001 ttggccttcg atgatactta catcatatcc gtttttctca agtacggccg ctatataaca
    39061 aaggcccagc ggcgggataa tatttagcac agctttcatg ctttctgtct tgcccgcgct
    39121 ttcctcttct ccaaaaggcg gtgaaatcaa cactatctta gtcatcttca ctaacctccc
    39181 tcactatgcg gttctttaca gcgatctttt tttcctgaag taagccgatt atctccttat
    39241 atacatgatc agcgctaatt tttctcatgc attcgatatc tttatcacaa ttttgtttgt
    39301 agcagggcgc gcaatcaagg tcagacataa ttctccttcc atccccatac agctctatct
    39361 cattaggatt aacgcatccg aaaatagcta tcaccagcct gttttgtgcg acagctatat
    39421 gcactgcaga ggtatctcct gatactatca ggtcgcagct gccgacaagc gctgcaaaat
    39481 cgtcgattgg atggactcct ccgttgatga tcggaacctg gcttttcagt gctatttctc
    39541 gattcttctc ctgctctgaa gggccgccta gcaatattac tcttgcatta aggttattat
    39601 aaagcagctc tgcaagcccg acaaagcctt ctattgtcca ttccctgttt ggaaagactc
    39661 tttttgagca tcctgtgtta aatccaacaa ccaagtcctc gcttttaatg ttatttttta
    39721 ccagaaactt tttggcgtta tctaatgctt cctttgagag gatcaaggtt ggtctttggc
    39781 cattatactc catctcactg atctcaaaca taagctgctg ctgggttttt ttattcgcta
    39841 tcttcagctc atcagaaagg cccatatcca ttgcagcctg cgatccaggg ttcatggcaa
    39901 agataagtcc atcttcctta agcccaaaac ctagcttggt gtcagccctg atcaggcagg
    39961 aaagggctgc cgcttcagca gatttgtcaa gattgatcaa gacatcaaac ttggaagctt
    40021 gtagtctcag ggagctctca aggtttaaag ccagcagttc gtcgattaag ggattatttt
    40081 ttagcagatc atagacattc tgggaagtca ggaatactat gcttgcacct tcatatttgg
    40141 ccttgagcgg ttcaagcaag gacgtggtcc taaggacatc tccaatacca accagattta
    40201 tcactagtat ccgggttttg actccatcat agaacctgca gctggcgcac acagaaccgt
    40261 cggacttatg ataagcacat ggcttgcttc ctttaaaaaa cctgcaatcc cttttggtct
    40321 ccatctgctt gcacccttaa ggggagagaa gaaggtctgt ttaaataatt ttgttttttg
    40381 tgtgcaagcg aaaattgact cttgcaaaag acccgcagcc aggacaaaaa tcagcgataa
    40441 ggatcatcca tcacaaaaca gtaatcgttt agctttatcc cactaattct aattatatac
    40501 ttgagcaccg aaatatttat ttagttcttg tagttacatt gtaactacat gagaaaacca
    40561 attcaaatga agatggaagg ttacgaagtt gttgagaaga
//
LOCUS       DASRUK010000110         4592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2254482, whole genome shotgun sequence.
ACCESSION   DASRUK010000110 DASRUK010000000
VERSION     DASRUK010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2254482"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VFF28_04920"
     CDS             <1..483
                     /locus_tag="VFF28_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44998.1"
                     /translation="LFVKDFLLIPLDISVRDFKLGDIAKFNILVENIGNIKVEGASSM
                     LLIDEKDGSPLANIKSISTDFSPLEKKEMVSYWDTKDVEADAYQGRLVLSYGSKSDEK
                     KIITNIGEGKIDVEIIGITGHAISRQSPEFSSKGMIISVIIVINVVWFIFYHYTRRKR
                     "
     gene            480..842
                     /locus_tag="VFF28_04925"
     CDS             480..842
                     /locus_tag="VFF28_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX44999.1"
                     /translation="MNIKNAISLALPIISIIAIAFFIRDPTGFAVLEDSSVYSGNITI
                     EFQGSFAKNSTIVLSVDDSSYNWSIIDFLDKGKIRYNLSNQKEEKIALHGRYLISTKD
                     LGLILRSSNHTFTIRLED"
     gene            1122..1568
                     /locus_tag="VFF28_04930"
     CDS             1122..1568
                     /locus_tag="VFF28_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45000.1"
                     /translation="MHERLSLLKVWQSAPHADYIISEIMEQAKKKSKPWVFNSLQSLV
                     KDNLLIYKKRGNLGFYSLNLDNPLLPQAFEQMPGNQGQTFLATIKDIIKNVSQDYCLF
                     EEDGTLILLTENKITFKGCKSASFREFSGKGNIIYNARFYFKNKRA"
     gene            1580..1840
                     /locus_tag="VFF28_04935"
     CDS             1580..1840
                     /locus_tag="VFF28_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45001.1"
                     /translation="MDKNKQTTALIIIAVALIAALGYIGLDSYNERKAQEQLNVYQNG
                     LQAGYTQAILQLMQQASTCQQVPIMFQNQSLNIIAVECLKEK"
     gene            1953..2023
                     /locus_tag="VFF28_04940"
     tRNA            1953..2023
                     /locus_tag="VFF28_04940"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1985..1987,aa:His,seq:gtg)
     gene            complement(2028..2543)
                     /locus_tag="VFF28_04945"
     CDS             complement(2028..2543)
                     /locus_tag="VFF28_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45002.1"
                     /translation="MIGYEQLLKKGFSKQEAMKTIGILAKAEQKKSARIRFLDSIIYW
                     VLLFVAIIGNMVIAIILIPFILTFRPVPLFFIIALLAAMFGFLMDTLIRDIESIEKKH
                     LIIAWLFIPSLAVINIFYMTSFTDHLVDVFSMSLTMPSPFFISFVYVMAFTLPYIFHD
                     QLQVFYKRMSG"
     gene            complement(2540..2986)
                     /locus_tag="VFF28_04950"
     CDS             complement(2540..2986)
                     /locus_tag="VFF28_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45003.1"
                     /translation="MIEESLEEAREGIKRVDHLFYVSLKYTRTVDMMRNLIDRIISTF
                     DASFDSLLQFAEQKKKIASAPETPATKAEAVSKLYPNTKKYTDLYLLLRKLTREPYTK
                     REEYRRHVTMISTVGDKIVETDIDILKDYYDKIAEFLRFVEETIRE"
     gene            complement(2983..3486)
                     /locus_tag="VFF28_04955"
     CDS             complement(2983..3486)
                     /locus_tag="VFF28_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45004.1"
                     /translation="MDKFEELRDSAKKKLQVADHMLTMTYPMVQDPKLLMAVMENLFL
                     ALSGSIGALLHFERIYKRVPPFQDTFVSKFTVFRQKCAERYNIGHEYLMMIEEIKELI
                     MQHKKSPVEFKRKNSFVICSESYNIQSISLEKMHSYISRSKVFVNMISDIIDEGMQLA
                     EKELRVA"
     gene            complement(3523..3861)
                     /locus_tag="VFF28_04960"
     CDS             complement(3523..3861)
                     /locus_tag="VFF28_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nascent polypeptide-associated complex protein"
                     /protein_id="HZX45005.1"
                     /translation="MIPGVNPRQMRQAMRKLGIQQQDIDATEVIIRTPDKEIVISNPQ
                     VAKVNMMGQETYQIVGQAEERSISKEPEINEDDIKAVIDQTGVSKEEAEQAIKKNNGD
                     LAAAILELKE"
     gene            complement(3858..4031)
                     /locus_tag="VFF28_04965"
     CDS             complement(3858..4031)
                     /locus_tag="VFF28_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004591976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit Sec61beta"
                     /protein_id="HZX45006.1"
                     /translation="MAGDKITMPSGMGGLVRYFDEYKSKIEFKPGHIVILCIVVIVIM
                     VLLYTYGNRLLGI"
     gene            complement(4089..4404)
                     /gene="rnpB"
                     /locus_tag="VFF28_04970"
     ncRNA           complement(4089..4404)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFF28_04970"
                     /product="RNase P RNA component"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00373"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00373"
BASE COUNT         1378 a          948 c          884 g         1382 t
ORIGIN      
        1 ctatttgtaa aagacttcct gcttatacct ttggatatct cagtcaggga ctttaagctt
       61 ggagacatag ctaaattcaa catcctcgtt gaaaacattg gaaacataaa agtggaaggc
      121 gcaagctcga tgcttcttat cgacgaaaag gatggctctc ctttggcaaa cataaaaagc
      181 atctccactg actttagtcc tcttgagaaa aaggagatgg tcagctattg ggacacaaag
      241 gatgtagaag cagatgccta ccaaggccgg ctcgtcttgt cctacggaag caaatctgat
      301 gagaaaaaga taatcacaaa tattggcgaa ggtaaaatag atgtagagat aataggtata
      361 acaggacatg ctataagcag gcaatcacct gaattttcat ccaaagggat gataatatct
      421 gtcatcattg tcataaatgt ggtatggttt atattctacc attacaccag gagaaaacga
      481 tgaacataaa aaatgccatc tctctagcgc ttcccattat ttccataata gccatcgctt
      541 tctttataag ggatccgacc ggttttgcgg ttttagaaga cagctctgtt tatagcggca
      601 atataaccat agagtttcag ggctcttttg ctaaaaattc gaccattgtc ttgtcagtcg
      661 acgattcatc gtataattgg agcatcatag actttcttga taaaggtaag atacgctata
      721 atctgagtaa tcaaaaagaa gaaaagattg ccctacatgg caggtacctt atatcaacaa
      781 aggacttagg cctaatactt aggagcagca accatacatt tactataaga ctggaggatt
      841 gatccataat gccttcgcag gttttctaaa gcgaatatca atccctcaat tatgtcagag
      901 cctaaaactg cggactttag tcctgtagat attacgctcg tctctctagc atcctaaaaa
      961 tccgcttgca gaagaaagtg tcctaaccat gcactatgtc tagacatttc agctgatttt
     1021 taggatattt ttctaagtaa tgatagttat cttaataaat attactaatt aacaactaag
     1081 ctacagttaa atatttaagt aagtaataaa ataatcaccc tatgcacgaa agactcagcc
     1141 ttctaaaggt atggcagagc gctccacatg ctgactatat catctcagag ataatggagc
     1201 aagcaaaaaa gaaaagtaag ccatgggtct ttaattcact gcaatctctt gtcaaggata
     1261 atcttttgat atataaaaaa agaggtaatc ttggcttcta cagccttaac ttggacaatc
     1321 ctcttcttcc tcaagcattt gagcaaatgc caggtaatca aggtcaaacc ttccttgcga
     1381 ccatcaagga tatcattaaa aatgtaagcc aggattattg cctttttgaa gaagacggca
     1441 cacttatact gttgactgaa aataagatca cttttaaggg atgcaagtca gcttccttta
     1501 gggaatttag cggaaaaggc aacataatat ataacgctag gttttatttt aagaataaaa
     1561 gagcatgagg tggaaaagaa tggataaaaa caaacagact acagcgctga taataatcgc
     1621 tgtcgcattg atagcagcgc ttggatacat aggattggat agctacaacg aaagaaaagc
     1681 acaagagcag cttaatgtgt accaaaacgg ccttcaggca ggttacactc aggctatcct
     1741 tcagctaatg cagcaggcat cgacttgcca gcaggttccg attatgttcc agaaccagag
     1801 cctcaatatc atcgctgtcg agtgcctaaa ggaaaaataa gatttaagca catttttaat
     1861 tttttttatc aggctataga taatatgcct aaagagaaga agtaaataag cagtaaagtt
     1921 tataagtggt ttattattct gaaagataga tggctgccat agtttaatgg tagaatacag
     1981 gactgtggat cccgtgaagc gggttcgatt cccgctggcg gcccttttta accagacatt
     2041 ctcttataaa aaacctgcag ctggtcgtga aatatatatg gcaaggtgaa ggccatcaca
     2101 tatacaaagc tgataaagaa gggcgaaggc atggtaagac tcatgctaaa aacatccacc
     2161 aggtgatcgg taaatgaagt catataaaag atgttgatga ctgcaagaga gggtatgaac
     2221 agccatgcga ttatcagatg ttttttttct atgctttcta tgtctcttat caaggtgtcc
     2281 atcaaaaagc caaacatcgc tgcaagaagg gctatgataa aaaataaggg tactggccta
     2341 aaggtcaata taaacgggat gaggattatg gctatcacca tattgccgat tatcgccacg
     2401 aaaagcagga cccaatagat tatagaatca agaaaccgga tccttgctga ctttttttgc
     2461 tctgcttttg ccagaatccc tatagtcttc atcgcctcct gcttactaaa tcctttcttc
     2521 aggagctgct cgtagcctat cattctctta tagtttcctc tacaaagcgc aaaaactctg
     2581 caatcttgtc ataataatcc ttcaatatat ctatgtcagt ctcaacgatc ttgtcgccga
     2641 cagttgagat catggtgaca tgccgcctat actcttcccg tttggtatag ggctctcttg
     2701 taagtttcct tagcagaaga tataaatcag tatatttctt ggtgtttgga taaagcttgc
     2761 ttacagcctc tgcctttgta gcaggggtct ctggggctga agcaatcttt ttcttttgct
     2821 ctgcaaattg caataatgaa tcaaaagaag catcgaatgt gcttattatc ctatcgataa
     2881 gatttctcat catatccacg gtgcgggtgt atttcaggct gacatagaaa agatgatcca
     2941 ccctttttat cccctctctt gcctcttcaa gcgactcttc tatcatgcta ccctcaattc
     3001 tttctctgcc aactgcatcc cctcatcgat tatatcactt atcatgttaa cgaaaacttt
     3061 agaccttgaa atgtagctgt gcatcttctc taaagaaatg ctttggatat tataactttc
     3121 tgagcatatg acgaaagaat tcttacgctt gaactcaaca gggctttttt tgtgctgcat
     3181 tataagctct tttatctcct caatcatcat aagatattca tgacctatat tatacctctc
     3241 cgcgcatttt tgcctaaaga cagtaaactt tgacacaaag gtgtcttgga agggtgggac
     3301 gcgcttatat atcctttcaa agtgaagcaa agcgcctatc gagcctgaaa gggcaaggaa
     3361 aaggttttcc atcactgcca tgaggagctt tgggtcctga accatggggt aagtcatggt
     3421 gagcatatga tctgcaacct gcaatttctt tttagcgcta tctctaagct cctcaaactt
     3481 atccattgta atacataatt gataagaagt ttaaataatt tgttattcct taagctccaa
     3541 tatggcagcg gctaagtcgc cgttattttt ctttatcgcc tgctctgcct cttctttgct
     3601 tacgcctgtt tggtcaataa cagcctttat gtcgtcttca tttatctcag gctcttttga
     3661 gatagaccgc tcttctgcct ggcctactat ttggtaggtt tcctggccca tcatattaac
     3721 tttagcgacc tgggggttag aaataactat ctccttatct ggggttctta tgataacctc
     3781 tgtggcgtca atatcctgct gctggatgcc aagttttctc atcgcttggc gcatctgcct
     3841 gggatttact cctggtatca tatgccaagc agcctatttc cataggtata aagaagcacc
     3901 attatgacga taacaacgat gcaaagaatg acaatatggc ctggcttgaa ctctatcttt
     3961 gatttatact catcgaaata ccttacaagc ccgcccatgc ctgagggcat tgttatcttg
     4021 tcgcctgcca taggtgatga taatatatcc ttatttaaat agttaacgac tttgtaagaa
     4081 aaaaggcagg gttggaataa gccacctttt gtcagatgat aatcatctgc cttagcattt
     4141 aactaagcgg cttttttgcc ttgcaccggc caggtctcgc cgtttcatcc attccctaaa
     4201 gagcgtcagc cggttatctc agcttcgtcg ccaaagcctt ctcgactttc atcctcctct
     4261 aaggtggtct cgtttctgtg cggttgccgc acatcactgc accttcttta tgaagtggcc
     4321 ataacagcaa tttcttgctg ctggtgggtg gactttcctc agttcatact ccttgcgaag
     4381 gaaccgttag ctaagtacca tccctgcttg aacttcgaaa tatatttata tttataaagt
     4441 taatggaaaa acgtaatcgt ttagctttat cccactaatt ctaattatat acttgagcac
     4501 cgaaatattt atttagttct tgtagttaca ttgtaactac atgagaaaac caattcaaat
     4561 gaagatggaa ggttacgaag ttgttgagaa ga
//
LOCUS       DASRUK010000111         2092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1269002, whole genome shotgun sequence.
ACCESSION   DASRUK010000111 DASRUK010000000
VERSION     DASRUK010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269002"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VFF28_04975"
     CDS             complement(<1..325)
                     /locus_tag="VFF28_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HZX45007.1"
                     /translation="MTLKSLTGKDAIIIVDRGNTAIFHSLEIAKKLGKTQVVIQDQGA
                     WLVYKQYPKKIGLELIVLKTDYGLIDAKTLAASIDKDSALLITSMAGYIAVQDMDAIY
                     NICKKK"
     gene            complement(356..1240)
                     /locus_tag="VFF28_04980"
     CDS             complement(356..1240)
                     /locus_tag="VFF28_04980"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HZX45008.1"
                     /translation="MTFKEQIEKIKENWLILLIAVIGILLVAGSGSISNKMARNFESL
                     AYGGAYDKDIAQEAYYRGAIAPAPSSDFAPEVDERKITKSASLSAEVKRGLFTEADSQ
                     VKAIIKSSNSYLLSENSNKYGRGLGQYRVGSYQIKVEQEKYDSVLAQLKTIGKITSFN
                     GNALDITGSYTNAQINLETEEARLQRYQKLYAEATSITDKITLEDKIFDQERRVKYLK
                     DSIEKMDQKIDYSTIYLSINEKQSGYANVVFVKLSEIIRGFVSSVNNLLMFMAYILPW
                     AVAVFIVSWVYRLHKKKK"
     gene            1378..1608
                     /locus_tag="VFF28_04985"
     CDS             1378..1608
                     /locus_tag="VFF28_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5654 family protein"
                     /protein_id="HZX45009.1"
                     /translation="MKDEVIDKLSSLITAAFGLVAALAWNTAIQAIFTRIFGTDSSIP
                     AMISYAVIVTFIAVGATVWLGRISERAKKINL"
     gene            1672..>2092
                     /locus_tag="VFF28_04990"
     CDS             1672..>2092
                     /locus_tag="VFF28_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45010.1"
                     /translation="MAYFETEIEGIQCIVNVMKTENPDRYVQQIASAAHKLHEKTKGI
                     DYARMGELAGMEDSHTSMLRARERFSINNCIITGWPGTIQACLEVTAPDGNKSRITIG
                     ENGMRYLGASSSNGFDKGNLPLGALLCAGYFLPAERTA"
BASE COUNT          567 a          420 c          430 g          675 t
ORIGIN      
        1 ctttcttttt gcagatatta tatatagcat ccatgtcctg gactgcgatg tatcctgcca
       61 tcgaggtgat gaggagggca gagtctttgt cgatggaagc tgcaagggtt tttgcatcta
      121 tcaagccata gtctgtcttt aacacaatca attctaatcc aatctttttt ggatactgct
      181 tgtagacgag ccatgctccc tggtcttgga taacaacctg tgtctttcca agcttttttg
      241 ctatctcaag agaatggaat atggctgtgt ttcctctgtc gacaattatt atagcatcct
      301 ttcctgtgag ggattttaac gtcatccttc acaggctagt atgacgcttt attaattatt
      361 tctttttttt gtgcagccta taaacccaac taactataaa gacagccact gcccagggca
      421 atatgtatgc cataaacatc aacaggttgt ttacgcttga gacaaatcct ctgataatct
      481 cagagagctt gacaaacact acgtttgcat agcctgactg cttttcgttt atgctaaggt
      541 atatcgttga gtaatctatc ttctggtcca tcttttctat ggaatcctta agatacttta
      601 ctcttctttc ctggtcgaat atcttatctt caagggttat cttgtctgtg atgctcgttg
      661 cctcagcata taacttttga tatctttgaa gccttgcctc ttctgtctca agatttatct
      721 gagcattggt gtagcttcca gttatgtcca atgcgttgcc gttgaaggag gtaatctttc
      781 ctatagtttt caattgagcg agtactgaat catatttttc ctgttcaact tttatctggt
      841 atgagccgac gcgatattgg cctaaacccc tgccatactt gtttgagttc tcgctgagca
      901 aataagagtt tgatgatttg attatcgctt tgacctgaga atcagcttct gtaaacaagc
      961 ctcttttcac ttcagccgat aaagaagcgc tctttgttat ctttctttcg tcaacttctg
     1021 gggcaaaatc agagcttggc gctggagcaa tcgcccctct atagtaagcc tcttgtgcaa
     1081 tatctttatc ataagcgccg ccataggcaa ggctttcaaa atttcttgcc atcttgttgg
     1141 agatgctgcc tgaaccggct accagcagaa taccgataac tgctatcaac aatatcaacc
     1201 aattttcctt tatcttttct atctgttcct tgaatgtcat tttatcacct atcaacgcta
     1261 attgcgtaag ggctatataa atattgttta gactatcaaa tggattgaag ctatgacggt
     1321 ctgacggcgg ataacttaat atagctgtag aacaataaag ctttaagggg tgatactatg
     1381 aaggacgagg ttatcgataa gttgtcttct ttgataacag ctgcgtttgg gcttgttgct
     1441 gcgcttgcat ggaacactgc aatacaggct atctttacaa ggatatttgg gacagacagc
     1501 tcgattcctg cgatgatatc atatgctgtt atagttacat ttattgctgt aggtgcaaca
     1561 gtctggctag gcaggatatc tgaaagggca aagaagataa atttataatt tatataaaat
     1621 ataaattgta agctataaat atttttaaat agttatctaa actatattct catggcttat
     1681 tttgaaacgg aaattgaagg tatccaatgt atcgtcaacg tgatgaagac agaaaaccct
     1741 gacagatacg ttcaacagat agcctctgct gcccataaac tgcatgaaaa aacgaagggg
     1801 atagactatg caagaatggg agagttggct ggcatggagg actcacacac aagcatgcta
     1861 agggcaagag aaaggttttc gataaataac tgcattatca ctggatggcc aggaactatc
     1921 caggcctgtc ttgaggttac tgcccctgat ggcaacaagt caaggataac aataggagaa
     1981 aatggtatga gatatctcgg cgcatcttct tcaaacggtt ttgataaggg caatttgccc
     2041 ctgggggctt tactttgcgc tggctatttc cttccggcag aaaggacggc ag
//
LOCUS       DASRUK010000112         3876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2254532, whole genome shotgun sequence.
ACCESSION   DASRUK010000112 DASRUK010000000
VERSION     DASRUK010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2254532"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VFF28_04995"
     CDS             <1..337
                     /locus_tag="VFF28_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45011.1"
                     /translation="IQAAAAGEEQQAEEILTNDSSVITRYQIYTLPGCEKCHAANEYL
                     SAKADGDVVDLSSDDGLKQFREVYKQLKDKIERNKDGSLPIPTILLYDGSDNLVTVAH
                     HVDQVKQLI"
     gene            334..1014
                     /locus_tag="VFF28_05000"
     CDS             334..1014
                     /locus_tag="VFF28_05000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HZX45012.1"
                     /translation="MKIKGLQKLSLIDFSPFSSCVIFLCGCNFRCGFCHNPEIVEGKG
                     KDISEDELFKFLAERKKWLDGVCITGGEPCINEELIEFIKKIKDMGYKVKVDTNGTNP
                     LMVEKLVKTADYIAMDIKTSPGRYDKATGVEADIVSIKKSAKIIMESEIDYEFRITVV
                     PGILDKEDIVEIGKWLKGAKNLFIQQFSNKICLDKSFEKVKPYSLEELDELKRTAEPY
                     FGKVEIRS"
     gene            complement(1079..1270)
                     /locus_tag="VFF28_05005"
     CDS             complement(1079..1270)
                     /locus_tag="VFF28_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45013.1"
                     /translation="MPKPDLDRIHIEKLAKMIQVMISKKAPNYAIFCETLEPGTNGLE
                     HLSAKLQELSSDAMHAIFD"
     gene            1362..1550
                     /locus_tag="VFF28_05010"
     CDS             1362..1550
                     /locus_tag="VFF28_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007043766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HZX45014.1"
                     /translation="MECRKAVNLKKCPCLHNECEHHGICCECLHYHLSLKELPACAFP
                     AHIARTDERSFEVFKGLQ"
     gene            1645..2124
                     /locus_tag="VFF28_05015"
     CDS             1645..2124
                     /locus_tag="VFF28_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HZX45015.1"
                     /translation="MNDNRPSWDDYFMKIAMLVAERSTCRRHHVGAVIVKDKRILTTG
                     YNGAAAGVKDCLLLGCLRDKNNIPSGERHEICRAIHAEQNAIIQAGLHGVNIEGSTIY
                     CTHPPCILCAKMLANARVKKYVTYGEYPDTSARELLVEVGIAFSKINKPLSQIYTLE"
     gene            2178..2723
                     /locus_tag="VFF28_05020"
     CDS             2178..2723
                     /locus_tag="VFF28_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45016.1"
                     /translation="MGELKLSFFAVDVILLVVFLGLTRTVFRLSGIGFVLELLALIIL
                     LFAAFIALVPAYSGSRGGWGFLAIVFAVIFIDLLFIFLATGRAGTAFTGALLFSALGL
                     LVSVLNVRKKEKPVMYEEKPAHEEVYTNFEPGKYVASKTGTTYHAPKCEWALKIKKKN
                     QIWFDDEKEAKKRFSPHSCIK"
     gene            complement(2744..3205)
                     /gene="lspA"
                     /locus_tag="VFF28_05025"
     CDS             complement(2744..3205)
                     /gene="lspA"
                     /locus_tag="VFF28_05025"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HZX45017.1"
                     /translation="MEKMKGRDGKLFALIAFSLVILDQLTKLLVVRSIGLHQKIDLIP
                     NFLSFTYVQNTGAGFGILQGQNLLLAFIALIIIGLILFYIKNILANRYYTICFALIFS
                     GALGNLTDRIFRGYVVDFVNLAIWPAFNVADSAISIGAVLLIIFSWKTDKK"
     gene            complement(3177..3614)
                     /locus_tag="VFF28_05030"
     CDS             complement(3177..3614)
                     /locus_tag="VFF28_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45018.1"
                     /translation="MGLFGKIAFWKKKDDFDFGKDFGADMKDMGMPQTPGMPNFDSTD
                     QNFGTPADNFSVQGNFGMQDNSFPSQPQYNPPQPPGRPVPMRQPFTPPMQPAQPTERD
                     YQNDKFEVLSAKLDSVRYTLESINQRLANLERAAYGENERKRW"
BASE COUNT         1185 a          733 c          877 g         1081 t
ORIGIN      
        1 gatacaggca gctgccgcag gtgaagaaca gcaagctgaa gagatactta caaacgacag
       61 cagcgtcatc accagatacc agatctacac cttgcctgga tgcgaaaaat gccatgctgc
      121 aaatgaatac ctttctgcca aggcagatgg cgatgttgtc gacctgtctt ctgacgacgg
      181 gcttaagcag tttagggaag tatacaagca gcttaaggac aagatcgaaa ggaacaagga
      241 tggaagcctg cctataccaa ctatattact ctatgatggg agcgataatc tggtaacagt
      301 ggcgcaccat gtggaccagg tcaagcagct tatatgaaaa taaaagggct gcagaagctg
      361 tctttgatcg atttttctcc tttttcttct tgtgttatct ttctttgcgg ctgcaacttc
      421 agatgcggct tttgccataa tcctgagatt gttgagggca agggtaagga tatatctgag
      481 gacgaactgt tcaaatttct tgctgagaga aaaaaatggc ttgacggtgt gtgcataaca
      541 ggcggagagc cttgcataaa tgaagagctg atagagttta tcaaaaaaat aaaagatatg
      601 ggctataaag taaaggtaga taccaacgga acaaatcctt tgatggtaga gaaacttgtc
      661 aagacagcag attatatcgc catggatatc aagacaagcc ccggcaggta tgacaaagca
      721 accggcgtcg aggctgacat agtgagcata aaaaagagtg caaaaattat catggagagc
      781 gagatagact atgaatttag gataactgtt gtgcctggaa tattggataa ggaagatatc
      841 gttgagattg gcaaatggct taagggggca aagaatcttt ttatccagca gtttagcaat
      901 aaaatctgcc ttgataagag ctttgagaaa gtaaaacctt attctttgga agagttggat
      961 gagcttaaga ggacagctga accttatttt gggaaggttg agataagaag ctagccttgc
     1021 tcatattcgc tcagcctttt ccaaatgtaa ttgttttctt ctttggttgc ttcaaggtct
     1081 aatcaaatat tgcgtgcatc gcatcagatg ataactcctg caatttagct gaaagatgct
     1141 ctaagccatt tgtgcctggt tctagtgtct cgcaaaaaat cgcgtaattt ggagcctttt
     1201 tacttatcat cacctggatc atctttgcca acttttcaat gtggatacgg tcaagatctg
     1261 gttttggcat agggacagtt gacggcaaaa aaagttataa agctgccgat agcttcaatt
     1321 gccacccaaa agtttaaaaa tataatcacc aaacttgatt catggaatgc aggaaggccg
     1381 tgaatcttaa aaaatgcccc tgcctgcata atgagtgcga gcatcatggg atatgctgcg
     1441 aatgcctgca ttatcatctg agccttaaag agcttcctgc atgcgctttt cctgcgcata
     1501 tcgcaagaac tgatgaaaga agctttgagg tattcaaggg attacaatga aaaaacttaa
     1561 atgcaagaca gtgacaggca aggtatgcaa gatagtgaag ggaaaggtca agtgccataa
     1621 agaaaagata aaggtgtgca taagatgaac gataacagac cctcttggga tgactatttt
     1681 atgaagatag caatgcttgt cgcagagaga tctacatgca gaaggcatca cgtaggcgct
     1741 gtcatagtca aagataaaag gatacttaca acaggataca acggagctgc tgccggagta
     1801 aaagactgcc ttctccttgg atgcctcagg gataagaata atatcccttc aggtgaaagg
     1861 catgagatat gcagggctat acatgctgag cagaacgcta taatccaggc agggcttcat
     1921 ggggtgaata ttgaaggatc gaccatatac tgcacccatc cgccatgcat actttgcgca
     1981 aagatgcttg ccaatgcaag ggtcaagaag tatgtgacat acggcgagta tcctgatacg
     2041 agcgcaagag agctgcttgt agaggtagga attgcgtttt ctaaaataaa caagccccta
     2101 tctcagatat atacgctgga ataatcacta ctttagaata gaaatattta tatatcagat
     2161 agccattagc aaaggttatg ggggagttaa aattaagctt ttttgcggtg gatgtcatct
     2221 tattggtagt attcttaggg cttacaagaa ctgtattcag gctaagcggc atagggtttg
     2281 tacttgaact gcttgcgctg atcatattat tatttgcagc atttatcgct cttgtccctg
     2341 cttattcagg ctcaagaggc ggctggggat ttttggcgat tgtatttgcc gtcatcttta
     2401 tcgacctgct ctttatattc cttgcaacag gcagggcagg aactgcattt acaggagcgc
     2461 ttttattttc agcgctggga ttattggtgt cggtcttaaa cgtgagaaag aaagaaaagc
     2521 ctgtcatgta tgaagaaaaa ccagcccatg aagaggttta cacaaacttt gagccaggca
     2581 agtatgttgc aagcaagaca ggaacaacat accatgcacc taaatgcgag tgggcgctaa
     2641 agataaagaa gaagaaccag atatggtttg atgacgaaaa agaggctaag aaacggttta
     2701 gccctcatag ctgcatcaag tgaacctatc atctcttgtg gttttatttc ttgtcggttt
     2761 tccaactaaa gattataagc aaaaccgctc ctatgcttat cgcagagtct gcgacattga
     2821 atgcaggcca aattgcaaga tttacgaagt cgacaacata gcctctgaat attctatccg
     2881 taagattacc cagggctcct gaaaatatca atgcaaagca gatagtataa tacctatttg
     2941 ccaggatatt tttgatataa aataggataa gtccgattat gatcagtgct ataaaagcaa
     3001 gcagaagatt ttggccttga aggatgccaa agccagcacc tgtattttga acgtaagtaa
     3061 agcttagaaa gtttgggatc agatctatct tttgatgaag gccgattgac cttacaacaa
     3121 gaagtttagt cagttggtca aggattacaa gtgaaaatgc tattagtgca aaaagcttac
     3181 catctcttcc tttcattttc tccataggct gcacgctcaa ggtttgccag gcgctgattg
     3241 atgctttcaa gtgtatatct tacagaatca agctttgctg acaaaacttc gaacttgtcg
     3301 ttctggtagt ctctttctgt aggctgagct ggctgcatag gaggagtaaa tggctgtctc
     3361 atcggcacag gccttcctgg aggctgaggc ggattgtatt gaggctgaga aggaaaacta
     3421 ttgtcctgca tgccaaaatt accttgaaca gagaaattat ctgcaggagt gccaaaattc
     3481 tggtcggtgc tgtcaaagtt gggcatccct ggagtctgcg gcatgcccat atccttcatg
     3541 tctgcgccaa aatcctttcc aaagtcaaaa tcatcctttt tcttccagaa tgcaattttt
     3601 ccaaatagtc ccattttacc tcttttttac tttatcccat gtttcatctg actgtataac
     3661 ataggatgta accttatcct ttgctttacc caagtagctt tcccctttgc ctaatatata
     3721 attctttcca ttttcggtaa tcgtttagct ttatcccact aattctaatt atatacttga
     3781 gcaccgaaat atttatttag ttcttgtagt tacattgtaa ctacatgaga aaaccaattc
     3841 aaatgaagat ggaaggttac gaagttgttg agaaga
//
LOCUS       DASRUK010000113         3521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1269673, whole genome shotgun sequence.
ACCESSION   DASRUK010000113 DASRUK010000000
VERSION     DASRUK010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269673"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            305..973
                     /gene="rsmI"
                     /locus_tag="VFF28_05035"
     CDS             305..973
                     /gene="rsmI"
                     /locus_tag="VFF28_05035"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009231469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HZX45019.1"
                     /translation="MPLYIVSTPIGNLKDITLRAIDTLKESDIILAEDTRRTGILLNH
                     YDIRQKRLISYNDWNKEKKTPYIIELLKANQHISIVSDSGTPAISDPGFYIARESIKN
                     TIQVIPIPGPSAAIAALVASGFPTDSFEFHGFIPKKEKAKRDFFESIKDNNKTTIIYE
                     SPYRIKKTVKALAEMLPERNICIARELTKKFEEFIRGSSKEVYEKIKDEELKGEITII
                     LDKV"
     gene            complement(970..1665)
                     /locus_tag="VFF28_05040"
     CDS             complement(970..1665)
                     /locus_tag="VFF28_05040"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HZX45020.1"
                     /translation="MAIKRISSGISNLDPLIDGGFVKNSVNLVAGGAGCGKSIFAMQF
                     IMEGLKKKEPGIYITFEEKKKRVYEDMLEFGWELERYEKKGLFVFLEYTPEQVKKILT
                     EGGGIVEAIIEKLKARRIVIDSITSFSLLYEDELTKKEAALALFELIEKWDCTGILTS
                     QDESKGGNTITAALEFEVDGIILMYHVKKKGVRQRAMEILKMRGTKIPEQTFELEITG
                     KGIKINPNKIEDL"
     gene            complement(1656..2378)
                     /locus_tag="VFF28_05045"
     CDS             complement(1656..2378)
                     /locus_tag="VFF28_05045"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HZX45021.1"
                     /translation="MNNRIKTFIPGLDEMLKGGLREGTNVLITGSPGTGKTLLGIQFI
                     LEGAKNGDAGVYITCEETVEDIRDYAEAIGYNIRSLEKKGLVTLIKQPISAKKLMTIA
                     TPLSVIESKSVKRVVLDSITLFGFVHASGEMDYRKEVLDFVLRMKESKVNLLVISEKS
                     ISNIDNIRYDTEDFLFDGLIILAKIRKGASFERCVMISKMRGQDHLFDLFPFSIDKSG
                     LKIFPKQMPFSLIEKDEKSRWQ"
     gene            2549..2686
                     /locus_tag="VFF28_05050"
     CDS             2549..2686
                     /locus_tag="VFF28_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2080 family transposase-associated protein"
                     /protein_id="HZX45022.1"
                     /translation="MKQTQIKLNGYEMIEKQVNKSGNSGRVYVPVEWVGKKVKIVLLS
                     Q"
     gene            2683..>3521
                     /locus_tag="VFF28_05055"
     CDS             2683..>3521
                     /locus_tag="VFF28_05055"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZX45023.1"
                     /translation="MKAQLSTFKDKYQDKSRDEILEEMFDLYEEKEKLEKELKKYKNA
                     NTPSSANKHLKASTQGLRAKKGAKRGAPKKHKGATFIWPASDETRRAGEKLEPLYKEL
                     EEEIKNADVVQGDETSHSVNGINHWIWVFCNHLISLFKFKEQRGGDIVEQTLGKGFKG
                     KLVSDGWMTYTSYANENKVVHQRCLDHLRREVKHECKEKHPDLYSWCCDIYFMVRKGK
                     GYKQKKRRLAMHSRCKAELAMLIASMKSHRNLRKLASKIENGGDKWFSCILHPELPMD
                     NNEA"
BASE COUNT         1077 a          725 c          716 g         1003 t
ORIGIN      
        1 tttggttctg gcagccatag gggggcaaat accacagcga tgctgttatc aataatccaa
       61 acattgagat ttaataagaa gaatgtgctc ttgggattac aggaaatact aaataatcct
      121 gtacaaagct aaacgattac gttttaattt gttggaaata ccctagctca cttgtagcga
      181 ctgggtgatt ggaatccaaa tcattatgca aggtatttac aacattaaaa atcccgcccc
      241 ttggggccat tgggattttt attttaatca aattaatata tgggatatac tctacccttg
      301 gattatgcct ctctatatcg tctccacacc gattggaaat ctaaaggaca taactctaag
      361 ggctattgat accttaaaag aatcagacat tattcttgca gaggatacaa ggagaacagg
      421 aatactctta aaccattacg atataaggca aaagaggctg atctcataca atgattggaa
      481 taaggagaaa aagactccct atatcatcga gctgctgaag gccaatcagc atatatccat
      541 agtctcggat tcaggcactc ctgcaattag cgatccagga ttttacattg caagggaatc
      601 tataaaaaac accattcagg tgatccctat ccctggccct tctgcagcga ttgctgcatt
      661 ggttgcctct ggctttccta ctgattcatt tgagttccat ggctttatcc caaagaagga
      721 aaaggcaaag agggattttt ttgaaagcat taaagataac aacaagacga ccataatcta
      781 tgaaagcccg tacaggataa aaaagacagt aaaggctctt gcagagatgc tgccagagag
      841 aaatatctgt atcgcaagag agctgacaaa aaaatttgaa gaattcatca gaggctcatc
      901 aaaggaagtc tacgagaaga taaaagatga ggaactaaaa ggagagataa ctattatctt
      961 ggacaaggtc taaaggtctt cgatcttgtt aggatttatc tttatgcctt ttccggttat
     1021 ttcaagttca aaggtttgtt cagggatctt tgtccccctc atctttagga tttccatcgc
     1081 cctttgcctc actccttttt tcttgacatg atacatgagg attatgccgt ccacctcaaa
     1141 ttccaaagca gcagtgatag tattcccgcc cttagattca tcttgcgagg taagtatccc
     1201 ggtgcagtcc catttttcga tgagttcaaa taatgcaaga gcagcttcct tttttgtcag
     1261 ctcatcttca tacaagagag aaaatgatgt tatcgaatcg atgactatcc tccttgcctt
     1321 tagcttctcg atgattgcct cgactatgcc tcctccttct gtcaatatct ttttcacctg
     1381 ttcgggggta tactctaaga atacaaacag ccctttcttc tcatatctct caagctccca
     1441 tccaaactca agcatgtcct cgtatactct ttttttcttc tcttcaaacg tgatatagat
     1501 gccgggctct ttctttttta gtccttccat gatgaattgc atggcaaaaa tcgatttgcc
     1561 gcagccggcc cctcccgcga caagattgac gctatttttt acaaatcctc catctatcag
     1621 gggatcaagg tttgatattc cgctggatat cctttttatt gccatctgct cttttcatcc
     1681 ttttcgataa gtgagaaagg catctgtttg gggaatatct taagtccgct cttatcgatg
     1741 ctgaaaggaa aaagatcgaa caggtgatct tggcccctca tctttgatat catcacgcac
     1801 ctttcaaatg atgctccttt tctaatcttt gccagtatta ttaggccgtc gaacaggaaa
     1861 tcttcagtgt cataacggat attgtcaatg ttggagattg atttttcaga tatgactaga
     1921 agattaacct ttgactcctt catgcgaagg acaaaatcca ggacctcttt tcgatagtcc
     1981 atctctcctg atgcatgcac aaagccaaaa agggttatgc tgtcaaggac aactcgcttg
     2041 acactcttgc tctctattac agacagcggg gtggcgatag tcataagttt ctttgcagag
     2101 ataggctgct tgataagtgt aacaaggcct ttcttctcaa gtgaccttat gttgtaccct
     2161 attgcctctg cataatccct gatatcttcg actgtttcct cgcaggttat atacactcca
     2221 gcatcgccgt tctttgctcc ttccagtatg aattgtattc caaggagcgt cttgcctgtt
     2281 ccaggagatc ctgtaataag cacatttgtc ccttctctaa gtccgccctt gagcatctca
     2341 tcaaggccag gaatgaatgt tttgatcctg ttgttcaaat tctatcaccc tttgttaaat
     2401 tcagggcctg cgtatataaa cgttttgaaa ctttgtactt attaagctga actatattct
     2461 ttagtatcta cttcaagccc tttctatagg acctatatac tctatcaccg aaatctttat
     2521 taacaagtta gctacattgt agctaacaat gaagcaaacc caaattaagc ttaatggtta
     2581 tgagatgatt gaaaagcaag tcaacaagag cggaaacagc ggcagggtat atgttccagt
     2641 ggaatgggtt ggcaaaaagg tgaagatagt tttgctaagt caatgaaagc gcaactaagc
     2701 acatttaagg ataagtatca ggacaaatca agagatgaaa ttctagagga aatgtttgat
     2761 ctgtatgaag aaaaagagaa gcttgagaaa gaactcaaaa aatacaaaaa cgccaacact
     2821 ccttcctctg caaacaagca ccttaaagcc agcacacagg ggcttagggc aaagaaagga
     2881 gcaaagagag gcgctccaaa aaagcataaa ggggcaacct tcatctggcc tgcttctgat
     2941 gagacaagaa gggctggtga gaagcttgag cctctttaca aagaactgga agaagagatc
     3001 aagaatgcag atgtagtgca gggtgatgaa acaagccata gcgttaatgg aataaatcac
     3061 tggatatggg tattctgcaa ccacctcatc tcattattta agttcaagga acaaagagga
     3121 ggggatattg ttgaacagac tcttggcaag ggctttaagg gaaagcttgt aagcgacgga
     3181 tggatgactt atacatcata tgcaaatgag aacaaagtag tgcatcaaag atgccttgac
     3241 cacttgagaa gagaggtaaa gcatgaatgc aaagaaaagc atcctgacct ctatagctgg
     3301 tgttgtgaca tttatttcat ggtaaggaaa ggcaagggat acaagcagaa aaaaaggaga
     3361 cttgccatgc attcgagatg caaagctgag cttgcaatgt taattgcctc tatgaagtct
     3421 cataggaact taaggaagct tgcctcaaaa attgaaaatg ggggagacaa gtggttttcc
     3481 tgcatactac atcccgagct gcccatggat aataatgagg c
//
LOCUS       DASRUK010000114        10002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1433356, whole genome shotgun sequence.
ACCESSION   DASRUK010000114 DASRUK010000000
VERSION     DASRUK010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 10002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10002
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433356"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VFF28_05060"
     CDS             complement(<1..394)
                     /locus_tag="VFF28_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45024.1"
                     /translation="MTDSISVVMVPELLKDNGISFNPKQEDKMTHMNSLEVAHWSNEV
                     LEMLHCDFITGADLGNLVPDSNSKNGVFSCKLIQNFSEPFGNIRVIAHFSPISLSSSS
                     SDILRCGPFIDFFNSSINSGFIGISSTGC"
     gene            complement(391..696)
                     /locus_tag="VFF28_05065"
     CDS             complement(391..696)
                     /locus_tag="VFF28_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45025.1"
                     /translation="MSYTRSAWKREYVFGDSHLYSFIGTDGNLWFFGKGCNDAGFEVI
                     LKEDAFEFVLSLLNQAGIDLTKVQTKKLAKELNVRLRKRPLTFEEMSKDMERRARKK"
     gene            complement(693..1793)
                     /locus_tag="VFF28_05070"
     CDS             complement(693..1793)
                     /locus_tag="VFF28_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45026.1"
                     /translation="MDSTSMITCPNCMSKSILKKGYRKNKMEKTPKYYCKGCGRYFVG
                     RKMLNKSYPANVILTAISLYNKGMTLEQASLEINKRFHVKTYPKLISTWLKEFSKVCA
                     FRRIRGKIREGRKSGFPNPVLEKLFDHKQPYLYKCHKLKLGMFLTEYYLGLKEYLLNV
                     QSICPNDLFIDDNARGSRIQFCHNTPILKKKNFACELAGLALKMAKTNRERHGIIQDF
                     MLANDTSTIAVEVPVWLSKEEVKRLDSEFNVFGFDSCITGHIDLVQARFGIIYLLDYK
                     PGAEKVDAVSQLFTYALALSERTGVWLRNFRCAWLDESVYYEFNPNEIILMNLEKKFG
                     RVPEAYLRKFVLDEKAKRYFTSKSFHEKRGGL"
     gene            complement(1786..3123)
                     /locus_tag="VFF28_05075"
     CDS             complement(1786..3123)
                     /locus_tag="VFF28_05075"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZX45027.1"
                     /translation="MDEINKESETKELKKSTSELKEAIISIVAILNKHQKGEICFGIN
                     NEGKIIGQEVSDATIREISKAISDFIEPKIFPRIEQAAIDDKQCISVIFEGYEKPYFA
                     YGRAYIRTGTENKQISARELENIILRKNREQVRWDTQICSKAALDDLDENTLKKFIKL
                     ATDSRRINKAGSSRYIIDKLGLMREGKLTNSAVLLFGKHPKEFFSNNWVKCGRFKGER
                     KEEFIDLKDFEGNLFQNLESSVNFLKEHLMISAKIEGMQRKEKWEIPLEAFREALINA
                     MIHRDYHAPGFIYIKVYDSKIIISNPGELPSQLDIEDLYTEHESVPRNPLLAETFYYT
                     GFIDVWGRGSLNIIRLLKENGIEKPLFEQSGGYFRAVFERPSATTQKTTQKTTQKILE
                     LIKENKFITRKELSEKIGISEDGIKFSLNQLKSRGLLNRVGPDKGGHWEVSDG"
     gene            complement(3116..5488)
                     /locus_tag="VFF28_05080"
     CDS             complement(3116..5488)
                     /locus_tag="VFF28_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45028.1"
                     /translation="MIDRPKIRKLGRIANRLNSCAAAKKSLQSKIDSMKADEKDGKIS
                     KKELDNYLDERIKGMSRLEPVKFLDKYSKQKSRLLVQKKASVKKAAPVFAGVAVALLL
                     LYLLPLEKTGYGVVEKNLAIPITVDSRYSMPFDGIIHSLRLDGQMSSQGLLKIYLNGT
                     NSSYLVLDSSMLDIRAVSDTTSILAEGDLNLTLVRNNTGSIYDFYTDSFGSEHLCALW
                     QVGEESLCYGPTDCCSFAGHESIGPYDEHVYLSYGRYGAGEENTVGVQIIFANYSIGE
                     NPYSDVRYSEVAQAEANFAKTFKGACIETCSLPGFEASNLVFEAENSSVTIDSIRYSE
                     EVPGNAPILLNDIENLTIYTGEYANIDLSKYFDDDSELTYSASSDAAVITNSSALIVS
                     NFTGKKYLYFTASDGIYSTVSNLVEIKATEKPIPASDANITEQLITPKIRINAPVRWV
                     TIINASSLAVNITINISADAFDLSVSSSKERLSQVTIKDGGEAKNISSYNAQKRLDVI
                     EAEEEKLSERKDWIIANTPLAKDEISAVNEELLKLSNERNKLTGYAVVSGRGLFTRFF
                     ERLLDVDITGYAVKDKKDKNKSKEIEVVIEEPVSSAVVQYYTEAPTSTETLLSSTSKQ
                     IIISSDVHYENITAYTNVSPETASHKLKLYRIVNSSREAASFDRYDNNNNSLTDYIEW
                     VVPSLSNQTYELVIEISKAEHLDSNYGFVEDVYDHVKAQDGNWTLINSSHYLRVTFEQ
                     NLTSDRDITIYAKDPRIVNGSVLLNGTLVPLDVYQKKMRIDEIRRMLANG"
     gene            complement(5485..5736)
                     /locus_tag="VFF28_05085"
     CDS             complement(5485..5736)
                     /locus_tag="VFF28_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45029.1"
                     /translation="MGYMYGERKRTFKELQKIILKTLLKGKATAYDLTKANKLHFNVV
                     SHQLILLKGHDHITLDFEHNRFRLYAITEKGTKYLRKIA"
     gene            5984..6646
                     /locus_tag="VFF28_05090"
     CDS             5984..6646
                     /locus_tag="VFF28_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45030.1"
                     /translation="MNNLKNIREFVWKVIDTDIALKKDISRGILNTRAAASYIISTYK
                     LKVSIDSVISALRRYNITPAKKQELDVYSLLRKAEIRTITKMASLSLKKNEDTTLSLG
                     KTLPRIDFEGGELLRILEGSKRFKILFDKKSYTKMTELFKKTDIIDSNKNISMLEMIY
                     PKELEKTPGVFSVVSNELAEKDISIIDALICSNEHIIIVGEKDILKAFEVVNNLCSED
                     VK"
     gene            complement(6639..7268)
                     /locus_tag="VFF28_05095"
     CDS             complement(6639..7268)
                     /locus_tag="VFF28_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45031.1"
                     /translation="MGKLLIGLIIAVSLVLIIGGIIFFWPEPDINAPTPDTGYRPENR
                     EKLQEKEAKKAIEEAKDEAVDAASAGVWSEAVSASESSSENGKEVIEEPSASLQLLDF
                     EYEVVNEDLVKITSLTYHISEMKIPMNSELFLFIYDDDDESSLFGLVRDQVRIGYVDY
                     GQVISQTSSVSGYYQGDLSSQKNVKLALIGYFADQSYTLSTDSRNVLFT"
     gene            complement(7300..>10002)
                     /gene="ppdK"
                     /locus_tag="VFF28_05100"
     CDS             complement(7300..>10002)
                     /gene="ppdK"
                     /locus_tag="VFF28_05100"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014789950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HZX45032.1"
                     /translation="GGGQADGNESMKELLGGKGANLAEMAGHKELKLPVPPGFTITTE
                     VCTYYYNNGKKYPTGLNEEANTAIKKIEALMGNKFGDAASPLLVSVRSGARKSMPGMM
                     ETVLNVGLTEKTIPGLIKQSGGNERFVYDSYRRLIMMYSDVVMEKAEGIEPADEMGIR
                     KQLERIMDQIKKEKGYKSDTEITAEELKELCRQFKVKIKEVLGTEFPDDPEKQLWGGI
                     GAVFSSWNGKRAISYRRIEKIPDEWGTAVNVQTMVFGNMGDDSATGVAFSRNPGNGEN
                     QFYGEYLVNAQGEDVVAGIRTPAPINAYSQSDHNKNLETLEQRSPKLYTELYDYRNRL
                     EQHYKDMQDIEFTIEKGKLFMLQCRSGKRNGTAAVRMAVDMLKEKLITNEIAVGRVTP
                     EQLDELLHPVIDPKVELKHKVLAKGLPAGPGGASGQIVFTAHHAVEAKKHGKKVILVR
                     EETNPEDVEGMRAAEAILTARGGMTSHAALVARGWGKCCIVGCSAIHIDYAKKEMRIG
                     DRVFKEMEWITLNGTKGNVYEGQLPMMDAAEENMILNEFLKVCDGIRKLGVRTNADTP
                     DDARRARSYGAEGIGLFRTEHMFYGKGSEEPLFRLRKMIVSKTLEERQKALDELFPYV
                     KRDIKATIEAMDGLPVVIRLLDPPLHEFVPKEEDKLNALAKELKITLEDLKKRADSLH
                     ESNPMMGHRGVRLGVTYPEITEMQVRAIFESAAELLTAGKKPYPEIMVPVVCDLNELK
                     HQFKIIEQVYLEVLAKYNIKSIKYMKGTMIEIPRAALLADKISEIAEFFSYGTNDMTQ
                     MTFGFSRDDIGGFLQDYLAQGILKDDPFQTIDQEGVGEIIKIGIEKGRKTRKSLEVGI
                     CGEQGGDPRSVDFCHRAGMNYVSCSPFRVPIARLASAQAALKNK"
BASE COUNT         2622 a         2414 c         1836 g         3130 t
ORIGIN      
        1 ggcatcctgt ggatgagatg ccgatgaagc ctgagtttat tgaggagttg aaaaaatcga
       61 tgaatgggcc gcaccgcaag atgtcgcttg aagagctgga taaactgata ggactaaaat
      121 gagctataac gcggatattt ccgaagggct cagaaaagtt ctggataagc ttgcagctaa
      181 agaccccgtt cttgctattg ctgtcaggaa caagattgcc caaatctgcg cctgtgatga
      241 aatcgcaatg aagcatttca agaacctcgt tggaccaatg agccacctca agagagttca
      301 tgtgggtcat tttgtcctct tgtttagggt tgaaggaaat accgttatct ttgagcagtt
      361 caggcaccat gacgacgctt attgaatctg tcattttttc ctcgctcttc tttccatgtc
      421 tttgctcatc tcttcaaaag tcagaggcct tttcctaagc ctgacattca gctcttttgc
      481 aagcttcttc gtctgaacct ttgtcaaatc aatgcctgcc tgattaagca ggcttaagac
      541 aaactcaaag gcatcctctt ttaggattac ttcaaaacct gcatcattac agccctttcc
      601 aaaaaaccac agattcccat cagtgccgat aaagctgtag agatggctgt cgccgaaaac
      661 gtattccctc ttccaggcag accttgtgta actcataatc cacctcgctt ctcatgaaaa
      721 ctcttgctgg taaagtagcg ctttgccttt tcatccaaaa caaatttcct caaatacgcc
      781 tcaggaaccc tgccaaattt cttttcaaga ttcataagaa tgatttcatt cggattaaac
      841 tcataataca ctgattcatc aagccatgcg catctgaaat tgcgaagcca aactccagtc
      901 ctctcagata aggcaagcgc ataggtgaaa agctgtgata cagcatctac tttttcagct
      961 cctggcttat aatcaaggag atagattatc ccaaaccttg cctgcaccag gtcgatatgc
     1021 cctgtgatgc agctgtcaaa tccaaatacg ttaaactcag aatcaagcct tttaacctcc
     1081 tctttggaaa gccagacagg aacctctaca gcaatcgttg aggtgtcatt tgcaagcata
     1141 aaatcctgga ttattccatg cctctccctg ttcgtctttg ccatctttaa ggcaagccct
     1201 gccagttcac atgcaaagtt cttctttttt aaaatagggg tgttatggca aaattggatt
     1261 ctgctgcccc ttgcattatc atcaatgaac agatcatttg gacaaatgga ctgcacatta
     1321 agcagatatt ccttcaagcc aagataatac tctgtcaaga acatccctaa ctttaactta
     1381 tggcatttat agagataagg ctgcttgtgg tcaaagagct tttccaaaac aggatttgga
     1441 aatccagact ttctgccttc tctaatctta ccccttatcc tcctaaatgc acatacctta
     1501 gaaaattctt tcagccaggt gcttatcagc tttggataag tctttacatg aaacctcttg
     1561 tttatctcca aagacgcttg ctcaagagtc atccctttgt tgtaaagaga gatcgcagta
     1621 aggatgacat ttgcagggta ggacttgttc agcatcttcc taccgacaaa atatcttcca
     1681 cagcccttgc aataatattt cggcgtcttt tccatcttat ttttcctata gccttttttc
     1741 agtatgctct tgctcatgca gttagggcat gttatcatcg aagtgctatc catcagaaac
     1801 ctcccaatgc cctcctttgt ccggcccaac tcgattcaac agtccccttg atttcagctg
     1861 gttaaggcta aatttgatgc cgtcttcact gatgcctatc ttttccgaca gctcttttcg
     1921 cgtgatgaac ttgttttcct tgattagctc cagaattttc tgggtagttt tctgggtagt
     1981 tttctgggta gtcgccgagg gccgttcaaa tactgcccta aaatatccgc cggactgctc
     2041 aaacaagggt ttttctatcc cattctcttt cagaagccgg ataatattca ggctgcccct
     2101 gccccaaacg tcgataaatc cagtataata gaaagtttca gcaagcaatg gatttctagg
     2161 aacagactca tgctcagtat ataggtcctc aatatcaagc tgggagggca gttcaccagg
     2221 gttagagatg attatctttg aatcatagac cttgatgtag ataaagcctg gcgcgtgata
     2281 atcccgatgt atcattgcat ttatcaatgc ctctctgaaa gcttcaagcg ggatttccca
     2341 cttttccttt cgctgcatcc cttctatctt agcagagatc atcaggtgct ccttgagaaa
     2401 attaacagat gattccaggt tttggaaaag gttgccctca aaatctttaa ggtcgataaa
     2461 ctcttctttt ctctctccct taaaccttcc gcatttgacc cagttgttgc taaaaaactc
     2521 ttttggatgt tttccaaaca gcagcacagc cgaatttgtg agctttcctt ccctcatgag
     2581 gccaagttta tcaataatgt atctggaact ccctgctttg tttatccttc ttgaatccgt
     2641 ggcaagcttg atgaacttct tgagggtatt ttcatccagg tcatctaatg cagccttgct
     2701 gcatatctga gtgtcccacc taacttgctc cctgttcttt ctgaggatta tattctcaag
     2761 ctctcttgct gaaatctgct tattctcagt cccggtcctg atatatgccc ttccataagc
     2821 aaaataaggc ttttcgtagc cttcgaagat gacactgatg cattgtttat catcgatggc
     2881 agcctgctca attcttggaa atatctttgg ctcgatgaaa tcagaaatcg cttttgatat
     2941 ctcccttatg gtcgcatcag agacctcctg tccaataatc tttccttcat tgtttattcc
     3001 gaagcatatc tcaccctttt gatgcttatt taatatagct acgatggata tgatcgcttc
     3061 ttttaactca gaggttgatt tctttaattc cttggtttca gactctttgt ttatttcatc
     3121 catttgcaag catcctcctt atctcatcta tcctcatctt cttctggtag acatcaagcg
     3181 gaaccaaggt accattgagc aggacagacc catttacaat ccttggatct ttggcatata
     3241 tcgttatatc cctgtcagag gtaaggttct gctcaaatgt aaccctaaga taatgtgaag
     3301 agttaatcaa agtccaattc ccatcctgtg cctttacatg atcataaaca tcctcaacaa
     3361 aaccataatt agaatccaga tgctcagcct ttgaaatctc tatcacaagc tcatacgtct
     3421 ggttggatag cgaagggact acccactcta tatagtcagt cagtgaatta ttgttgttgt
     3481 catacctgtc aaaagaagcc gcctccctgc ttgaatttac aatcctataa agctttaatt
     3541 tatgacttgc agtctcgggt gagacgtttg tataagcggt tatgttctca taatggacat
     3601 ccgaggagat gattatctgt tttgatgtag aacttagtag tgtctctgta gatgtcggag
     3661 cctctgtata atattgcaca acagccgaag atacaggctc ttctatcact acctctatct
     3721 cttttgattt gttcttatcc ttcttatcct tgacagcata tcctgtaata tctacatcga
     3781 gtagccgctc aaaaaatctt gtaaacaatc ccctgccaga aacaacagca taacctgtca
     3841 gtttgttcct ttcgtttgaa agcttaagca attcttcatt tacagcagag atctcatctt
     3901 ttgccagagg agtgtttgca attatccagt cttttctttc acttagcttt tcttcctctg
     3961 cctctataac atctagcctt ttttgggcat tataagatga gatattcttt gcttcccctc
     4021 catcttttat cgtaacctgg ctgagccttt ctttgctact gctaactgaa agatcaaatg
     4081 cgtctgcact tatgtttata gtgatattaa ccgccagaga agaagcattg atgatcgtca
     4141 cccaccttac aggcgcattt atccttatct taggggtgat caactgttct gtgatatttg
     4201 catctgacgc agggatgggc ttttccgtgg ccttaatttc aactagattg ctcacagttg
     4261 aatagatgcc atcagaggct gtaaagtaga gatatttttt gcctgtaaag tttgaaacta
     4321 tcaaagctga ggaattggtt ataacagctg catcggatga tgctgaatat gtcaattcgc
     4381 tgtcatcgtc aaaatacttg gacaagtcga tgtttgcata ttctcccgta tagatagtaa
     4441 gattctcaat atcatttaac aggattggag cattgcccgg cacttcctca ctatacctga
     4501 tactatctat agttacagat gagttttctg cctcaaatac aaggtttgaa gcttcaaagc
     4561 cagggagact gcaggtctcg atgcaagccc ccttaaatgt cttggcaaaa ttagcctctg
     4621 cttgagcaac ctcagaatat ctaacatcag aataaggatt ctcgcctatt gaataattgg
     4681 cgaatataat ctggactcct acagtgttct cttctcctgc accatagcgc ccatagctta
     4741 gatagacatg ctcatcataa ggaccaatgc tttcatgacc tgcaaaagaa cagcaatccg
     4801 ttgggccata gcacaagctc tcttctccaa cctgccacaa agcgcaaagg tgctcagagc
     4861 caaaagaatc ggtatagaaa tcatatatgc ttccggtatt gttccttaca agagtaagat
     4921 taagatcgcc ctcggcaagg atagaagtag tatcgcttac agccctgata tccagcattg
     4981 agctgtcaag cacaaggtag gagctattgg tcccattaag gtatattttt agcaagcctt
     5041 ggctgctcat ctgtccatcc agccttaggc tatggatgat gccatcaaac ggcatagagt
     5101 atctgctgtc gaccgtgata ggtatagcaa gatttttctc aaccacgcca tatcctgtct
     5161 tctccaaagg aagcagatat aataagagca aagcgactgc aactcctgca aagacagggg
     5221 cagctttctt aacacttgct ttcttttgca ccagtaatct tgatttttgt ttggagtact
     5281 tatccaggaa tttaacaggc tcaagccttg acattccttt tatcctttca tccagatagt
     5341 tgtcaagctc ctttttgctt atctttccat ctttttcgtc tgccttcatg ctgtctatct
     5401 tcgactgaag gctttttttg gcagcagcac aagagttcag cctatttgcg atccttccaa
     5461 gcttccttat tttaggtctg tctatcaagc tatcttcctc aggtatttgg ttcctttctc
     5521 ggttattgca taaagcctga acctgttatg ctcgaaatca agtgtgatat gatcatgacc
     5581 ttttaggagg ataagctgat gggaaacaac attgaagtgc agcttgtttg ccttggtgag
     5641 atcataggca gtcgctttgc ctttcaaaag agtttttaga ataatctttt gcagctcctt
     5701 gaatgttctt tttctctctc cgtacatgta tcccatgccc tctaaccctt gggcttataa
     5761 atctttcgtc ttatgcattt aagttatgta taatcaaaag ctattagagc gattataaag
     5821 aactataatg gttattttat gactttttta taaaacttta gttcatataa tcgtttggca
     5881 ccgactatta agcgttccaa tactattgga aggttatttg acaaaaaacc gctaaatttt
     5941 taaaaaaagg catatttaaa ggtttaaggg cataagtcac tttatgaaca acctaaaaaa
     6001 cattagagaa tttgtctgga aagtgataga tacagacata gcgctaaaaa aggatatctc
     6061 cagagggatc ctcaacacaa gggcggcagc atcttatata atcagcactt acaagcttaa
     6121 agtctcgata gactcggtca taagcgcatt aagaaggtac aatatcaccc cggcaaagaa
     6181 acaggagctg gatgtctatt ccctgttgag aaaagcagag atcaggacca taacaaagat
     6241 ggcttccctt tccctaaaaa agaacgaaga cacgaccctc tcgctaggaa aaacacttcc
     6301 gaggatcgac tttgaaggcg gagagctgtt aagaatactt gaaggctcaa aaaggttcaa
     6361 gatattgttt gacaaaaaaa gctacaccaa aatgacagag cttttcaaaa agacagacat
     6421 catcgactca aacaagaaca taagcatgct tgagatgatc tatcccaagg agctggaaaa
     6481 gacccctggt gtattttcag ttgtatcaaa cgaactagca gaaaaggata ttagcataat
     6541 agatgcgctt atctgctcaa acgagcacat cataattgtt ggcgaaaaag acatcctaaa
     6601 agctttcgaa gttgtaaaca acctttgctc tgaagatgtt aagtgaacag aacatttcta
     6661 ctatcagtcg aaagcgtata gctctggtca gcaaaatacc caatcaaagc aagcttgaca
     6721 ttcttctgcg aactcaggtc tccttgataa tatccagaga cagaactggt ctgtgatatg
     6781 acctgcccat aatcgacata tcctatcctt acctggtctc ttacaagtcc aaagagtgag
     6841 ctttcatcat catcgtcata tatgaaaagg aatagctcgc tattcatagg tatcttcatc
     6901 tcagagatat gataggtgag gctggttatc ttgactagat cttcatttac aacctcatac
     6961 tcaaaatcca ggagctgcaa agaagccgac ggctcctcta tgacttcctt gccgttttca
     7021 gaactcgatt cagaagcgct tactgcttca ctccagactc ctgcactggc agcatctact
     7081 gcttcatcct tagcctcttc gatggctttc tttgcctcct tctcctgtaa tttctctcta
     7141 ttctcaggcc tatatcctgt atcaggcgta ggggcgttta tatcaggctc aggccaaaaa
     7201 aagatgatgc ctccgatgat taataccaaa gaaacagcta ttatcaagcc tatcaataat
     7261 ttacccataa aaagataata aaaaaagaat tatttaaatc tatttattct taagtgcagc
     7321 ctgtgcagaa gcaagccttg ctatagggac cctgaaaggg ctgcaagaca catagttcat
     7381 tcctgcccta tggcagaaat caacagacct tggatctcct ccttgctctc cgcagattcc
     7441 gacctcgagg ctctttcttg tctttcttcc tttctctatt ccgatcttga ttatctcgcc
     7501 cactccttcc tggtcgatcg tctggaaagg atcatccttt agtattccct gagccagata
     7561 gtcctgcaaa aatccgccta tgtcatccct tgagaatcca aatgtcatct gcgtcatgtc
     7621 gtttgtgccg taggagaaga actcagctat ctctgagatc ttgtctgcaa gaagtgcagc
     7681 cctagggatc tcgatcattg ttcccttcat gtacttaata gacttgatgt tgtactttgc
     7741 aagcacttct aaatacacct gttctattat cttgaactgg tgctttagct cgttaaggtc
     7801 gcacactaca ggcaccatta tctctggata aggtttcttt ccagcagtaa gcagttcagc
     7861 agctgattca aatatcgccc ttacctgcat ctcagttatc tctggatagg ttactccaag
     7921 ccttactcct ctgtggccca tcataggatt gctctcgtgc agagagtcag cccttttctt
     7981 aaggtcttca agagtgatct tcagctcttt tgcaagtgcg ttcagcttgt cctcttcctt
     8041 tggcacgaac tcatgcagcg gtggatcaag aagccttatg acaacaggaa ggccgtccat
     8101 cgcttcgatg gttgccttga tatctctttt cacataaggg aaaagctcgt ccagtgcttt
     8161 ctgcctctct tccaaagttt tagacactat cattttcctt agcctgaaaa gcggctcttc
     8221 tgaacccttg ccatagaaca tatgctctgt cctaaatagc ccgattcctt ctgcgccata
     8281 gcttcttgct ctccttgcat cgtctggagt gtctgcattt gtcctgactc caagctttct
     8341 gatgccgtcg catactttca aaaactcgtt tagtatcata ttctcttcag cagcatccat
     8401 catcggaagc tggccttcat agacattgcc ctttgtgccg ttcagagtta tccattccat
     8461 ctccttgaat actctatcgc caatcctcat ctctttcttg gcatagtcaa tatgtattgc
     8521 tgagcatccg acaatgcagc attttcccca tcctctggcg acaagtgcag catgagaggt
     8581 catccctcct cttgctgtaa ggattgcttc agcagctctc atgccttcaa catcttctgg
     8641 gtttgtctct tccctgacaa gtattacctt cttgccatgt ttctttgctt caactgcatg
     8701 atgcgccgta aacactatct ggccgcttgc tcctcctggt cctgcaggca gtccctttgc
     8761 aaggacttta tgctttagtt cgaccttagg gtctattaca ggatgcaaaa gctcatcaag
     8821 ctgctcaggc gttacccttc caacagcgat ctcattagtt atcagcttct ccttaagcat
     8881 gtcgacagcc atcctcacag cagcagttcc gtttctcttt ccagacctgc actggagcat
     8941 gaacagcttt cctttttcta tcgtaaactc gatatcctgc atgtccttat agtgctgttc
     9001 cagcctgttc ctataatcat acagttcagt gtagagcttt gggctccttt gctcaagtgt
     9061 ttccaggttc ttattatggt ctgactgaga gtaagcattt atcggcgcag gagtccttat
     9121 accagcgaca acatcctctc cctgtgcatt cacaaggtat tcgccgtaga attgattctc
     9181 gccgtttcca gggtttcttg agaaagcaac gcctgttgca gaatcatctc ccatgtttcc
     9241 aaataccatt gtctggacat tgacagcagt tccccactca tctgggatct tctctatcct
     9301 tctataagag attgccctct ttccgttcca tgaagaaaat acagctccta ttcctcccca
     9361 aagctgcttt tcaggatcat ctggaaattc agtgccaagg acctctttta tcttgacctt
     9421 gaactgtctg caaagctctt ttagctcttc agcagttatc tcagtatctg acttgtaccc
     9481 tttctctttc ttgatctggt ccattatccg ctcaagctgt tttcttatgc ccatctcatc
     9541 agccggctct attccctcag ctttctccat gacaacatca gaatacatca tgataagcct
     9601 tctgtaagaa tcatagacaa atctttcatt tccaccgctc tgttttatca agccgggaat
     9661 tgtcttttca gttagcccta cattaagcac ggtctccatc atccctggca tggatttcct
     9721 tgctccgctt ctgacagaaa caagcaatgg gcttgctgca tctccaaact tattccccat
     9781 caatgcttcg atctttttta ttgcagtatt ggcctcttca tttaatccag tagggtattt
     9841 tttaccatta ttataataat aagtgcaaac ctctgtggtg atagtaaatc ctgggggaac
     9901 tggcagcttt agctctttat gccctgccat ctcagcaaga ttagcccctt ttcctccaag
     9961 aagctctttc atgctttcat tgccgtctgc ctggcctccg cc
//
LOCUS       DASRUK010000115        13113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2256243, whole genome shotgun sequence.
ACCESSION   DASRUK010000115 DASRUK010000000
VERSION     DASRUK010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 13113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13113
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2256243"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VFF28_05105"
     CDS             <1..435
                     /locus_tag="VFF28_05105"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HZX45033.1"
                     /translation="LAPNFNPFVSGFYCTWVKLKHSTPKKTAFNLMFDKDEVFDIPIA
                     HGEGRFTTKDKLVLNQMISNGQLVFRYCDDIGNIADKFPVNPNGSLINIAAVCNKEGN
                     VMGIMPHPERASWMRQVPGRHLLGERAGPGRKIFESIKEYLR"
     gene            432..863
                     /locus_tag="VFF28_05110"
     CDS             432..863
                     /locus_tag="VFF28_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45034.1"
                     /translation="MSVELFVGLKIPDNTAITALHAIERMGYKIGKLKRENYYNFEID
                     GDENSFSLKIGKVDILVNANKNKYSTALAREEGAHHVLVKDSDDKCEGLIKSLHKLGF
                     PEIKSMQRGVLWAIYSDSSDTAKEIAEKLLYNRHYQEIEIL"
     gene            898..2430
                     /gene="guaA"
                     /locus_tag="VFF28_05115"
     CDS             898..2430
                     /gene="guaA"
                     /locus_tag="VFF28_05115"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HZX45035.1"
                     /translation="MLLVINYGSQVCHLIARRIRDFGVYSEIVPFDVSASEVERLAPQ
                     GIILSGGPSSVYDKNAPISDAKILELGVPVLGICYGQQLIGKQLGGKVIPNKIKEYGR
                     RILTIENKDKLFKGLGDKEEVWMSHGDAVEELPSGFITLASTESCENASIADLKRDIY
                     GVQFHPEVTHTVKGEKILRNFVFDICKAKKDFDIGKMSSRIIEDIKKEVGDKSVLMGI
                     SGGVDSTVAASLIHKAIGKRLHCILIDHGLVRKDEVKTIKEMYDKLSLNVEYVDASSV
                     FLGKLKGVTDPEQKRKIIGHTFIEVFEREANRLESKDKKIEYLGMGTIYPDRIESAQP
                     SKQADKIKSHHNLTLPEKMKLKVIEPINELYKDGVRKLGKEIDVPDELLMRHPFPGPG
                     LAIRILGEITSERLRILREADDIFISELRKNGLYDKVWQAFAALLPVKAVGVMGDSRT
                     YEYIISLRAVCSIDAMTADWAKLPPEFLELVSNRIINEVKGVNRVLYDVTQKPPGTIE
                     YE"
     gene            complement(2717..3280)
                     /locus_tag="VFF28_05120"
     CDS             complement(2717..3280)
                     /locus_tag="VFF28_05120"
                     /inference="COORDINATES: protein motif:HMM:NF014056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF99 family protein"
                     /protein_id="HZX45036.1"
                     /translation="MFKREIRTIGIDDSPFDKFKKGSVLVVGAIFRGGSLVEGILTTK
                     VAVDGSNSTQKIIKMINLSKFKLQLRCILINGIALGGFNVLDIEELNKKTNIPVIVVI
                     RKYPDFKKIEDALININKQEKFALIKKAGPVHQIGKIFIQLKGLSLAEARAILKLTCT
                     RSLIPEPIRVAHLIAGGIVTGESKGQA"
     gene            3353..5179
                     /gene="glmS"
                     /locus_tag="VFF28_05125"
     CDS             3353..5179
                     /gene="glmS"
                     /locus_tag="VFF28_05125"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HZX45037.1"
                     /translation="MCGIIGYVGNNRAVDVLLSGLKKLEYRGYDSAGIATISDGKIKT
                     KKDIGKIEEVNQKVNFSELSGNIGLAHTRWATCGKVTKENAHPHLDCTGKISIIHNGI
                     IENYGQLKEDLIKKGHNFSSNTDTEVIAHLIEDEYKGDLKEAVISAIKHLEGSFALGV
                     IHADKEELVAARYESPLIIGVGSGENFIASDVPAIMEYTKNIIYLGNKEVAVLRGDSV
                     SLFDAEGNPVEGKIEKIEWNAEQAQKKGFKHFMLKEIFEQPETITETINERVSDTELL
                     IGREIGLSAKEIKNIKRIIIIACGTSWHAGIVGEFMIEEIARIPVEVEYASEFRYRDP
                     IVDKDTLVIPISQSGETADTLAALREAKKKGAKVVSIVNVKGSSIARESDSVIYTRAG
                     PEIGVASTKAFTAQLTVLYLLTVYFGSVKGVLPPEQVKNRIRDVKKLPLQIQSVLEDK
                     DYIRMHAEILYQKKNAIYLGRGINYPIALEGALKLKEVSYIHAEGYPAAEMKHGPIAL
                     IDKDMPVVVIATKDDRTYKKVLNNIEEVKTRGGIVIAIESKGDEEVKKIADHNFYVPQ
                     NSYILTPLLAVIPLQLMAYYIADLRGCNPDFPTNLAKSVTVE"
     gene            5253..6719
                     /locus_tag="VFF28_05130"
     CDS             5253..6719
                     /locus_tag="VFF28_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45038.1"
                     /translation="MKTVKFKSFVIKVKHNGTCFIRLPKRIAKKYPAKQEIKVLIDNK
                     IGFFCNIRTWRVIGFYVPATIAIKDNLFDKEVEVAIEKIDGFYSTLLPDGRVYLPNNL
                     AIKLKLRKQDIILLEFNDKKEYCTINFRKKGSTEEFFCMFAKQSINRQKGVFKIKKIF
                     RQRNFSVLSGKIHDDFAFDNCAQINKRRLLLFHGNRIPLLVDPCINIEKFAHYLGCYF
                     ADGNKKGNSWGICASTFKQAIYYKSMHERLIKDAQLTFGISYSNASDVGADTIKQELI
                     RKWQENTSIGLEGIDVRIITIKDEKVLKRNQYGSLILREDRDLTRLYYVRMMDLLLHK
                     ITETKNKALAIDFIHGVLEGDGSVGAKRGSLIITSNLTDLELLERVMKETRFVFHKRK
                     EGANKGFIYIGSLEIIRNISILKDNLFKFYPKRRKLLKVRLGDTGCARFLLGKSKKTS
                     NWLIGQLKEKGILDNDCKLTKEGQKIQKNLKQFLLSGD"
     gene            6721..7200
                     /locus_tag="VFF28_05135"
     CDS             6721..7200
                     /locus_tag="VFF28_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZX45039.1"
                     /translation="MMIRDATSMDARGIGAVLKECYNIASVEEGVLVFNSETAKGHHY
                     IVAEDNGKIVGLTTWLMHGLFRHQLCELDRIAVLADYRGKGAAKALFDALVVSADAFY
                     KKHGFKLRKLYLLTHADNLQAQRFYEKMGFSHETTLKSHYYDGKDEFVYSRFFRSFL"
     gene            complement(7184..7915)
                     /locus_tag="VFF28_05140"
     CDS             complement(7184..7915)
                     /locus_tag="VFF28_05140"
                     /inference="COORDINATES: protein motif:HMM:NF020287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HZX45040.1"
                     /translation="MTSKLLISREGNQFFVKDISKDYHCQYGFVSTSDLAKKDGSIIK
                     TNTGKELTLFSPSFIDLYTRIKRAPQIITRKDIGMIIAETGIGKISKVIDSGTGSGAL
                     SCFLANICKQVTSYELRPDFAEIAEKNRQMLDLTNLKIKNQDIYNGIDEKNIDVITLD
                     LPEPWKVIDHAKKALKHGGFLVSYSPTIPQVMDFISAIKKDSSLIHIKTTELMQRDWE
                     IEDRKVRPITTQTVHTGFLSFTRKI"
     gene            complement(7923..8051)
                     /locus_tag="VFF28_05145"
     tRNA            complement(join(7923..7958,7973..8051))
                     /locus_tag="VFF28_05145"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7974..7976),aa:Arg,seq:tct)
     gene            complement(8100..8234)
                     /locus_tag="VFF28_05150"
     CDS             complement(8100..8234)
                     /locus_tag="VFF28_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45041.1"
                     /translation="MATPKCCDGCLKWDQFGKKCWVYWEEKKQCTQKVESADQLMPMQ
                     "
     gene            complement(8268..9416)
                     /locus_tag="VFF28_05155"
     CDS             complement(8268..9416)
                     /locus_tag="VFF28_05155"
                     /inference="COORDINATES: protein motif:HMM:NF044723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HZX45042.1"
                     /translation="MKRITAKMKKESQSSLSEHSKKGTKDEVGSELVNLGKEIVKDIE
                     KGNNPKIELSVRGLSNVVYDKESKTLGLGDKTAQRFFFNVGHSKKFLQTIEVAALCKS
                     LLDVQKHASLRDVFYMAKRTIPSTNINIVDEQKESDKAIEDLEIITGYSRECLHVNAN
                     KMGSVAGKVIIEDMGDQIDWSRLGSGGWSIPSNVENIKFKKVDAKYVIYMEKAAVWER
                     LHEDKFWAKQNCIIMSSQGQTTRGIRRLLQRLSTEHNLPILVLTDFDSWGHYIYSVLK
                     FGSISLAHMADSLSVPNCRYLGIKADDIEKYGLQKHFIKLTDQDQSRLKQISAYEWFK
                     NSKQWQYEFKKMKQFGAKAEIQALSARGISFISEEYLPEKLKNKDWIE"
     gene            complement(9397..11034)
                     /locus_tag="VFF28_05160"
     CDS             complement(9397..11034)
                     /locus_tag="VFF28_05160"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003218.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HZX45043.1"
                     /translation="MSEETPIAMELAKKQREIGVAEFFARNRHLLGFDNKRKALMTTI
                     KEAVDNSLDACEEASILPEVSVEVIDMQNDRFRIVIEDNGPGIVKKQIPHVFARLLYG
                     SKFFKLSQSRGQQGIGISASVLYAQLTTGKPAKITSRISPKHDALFLELKLNTEKNMP
                     EIIKEEVIEWNKEHGTRIELELEGNYQGGFQSIDQYLKQTSIVNPHATIIYTNPKAEQ
                     MIFARATDTLPPKPVEIMPHPYGVELGVLIKMLGATESRTLQSFLMSEFSRVGAGTAK
                     EICENSSLMPSCKPKEINRDIAEKLLQGITKTKIIAPPTNCISPIGEELLEKGLQKEI
                     NAEFYCATTRPPSVYRGNPFIVEAGIAYGGEQSAEDTAILLRYANRVPLLYQQGACAV
                     TKSAIGTAWKNYGLGQSKGALPVGPVTIAVSINSVWVPFTSESKEAIAHYPEIIKEIK
                     LALQECGRKLQLHIHKKRHVQHQLERANLFERYMPEVADSLSRLTGENKEAILKKLKE
                     MVKKDDIQKEIYQMESLKGEVEDDTKLEKNSDEEDNS"
     gene            complement(11329..11883)
                     /locus_tag="VFF28_05165"
     CDS             complement(11329..11883)
                     /locus_tag="VFF28_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HZX45044.1"
                     /translation="MTEYLYELRIPKDRIAVLIGKEGKIKSEIEAETQTTIKVDSKEG
                     DIFITGEEALGLYSSREVIRAIGRGFNPEVAKLLLKGDYAFELIDLRDYVGKSKDHLL
                     RIKGRIIGAEGKTRRLIEELTGAHICVYGKTAGVIGEPTAASNAREAIEKLLRGSPHS
                     TVYKFLEKKRTMMKRERMLRADLE"
     gene            complement(11907..12617)
                     /locus_tag="VFF28_05170"
     CDS             complement(11907..12617)
                     /locus_tag="VFF28_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase RIO"
                     /protein_id="HZX45045.1"
                     /translation="MPIRSKEKFKTMHNVFDEFTNWTLFKLISEGYFEGLESPISIGK
                     EANIFSARRKDRSRVIVKIYRLEACDFNRMYDYIKEDPRYSNLKGKKRKIIFSWVQRE
                     YRNLLKAREGGVNVPTPITFKNNIIVMEFIGKADNVSSRLSVEAPKNPKKFFDKVVDN
                     IRKLYKAGLVHADLSPFNILNHDEEPMLIDFSQATILDNSRAMEFLQRDVRIVATYFT
                     KLGLKIDLGKVLKTIKNP"
BASE COUNT         3757 a         2507 c         2765 g         4084 t
ORIGIN      
        1 cttgcaccaa atttcaatcc gtttgtgtct ggattttact gcacatgggt aaagcttaag
       61 catagcaccc ctaaaaaaac tgcctttaac ctaatgtttg acaaagatga agtctttgat
      121 atccctatcg cgcatggaga gggaagattc accacaaagg ataagcttgt gcttaaccag
      181 atgatctcca acggacagct tgttttcagg tattgcgatg acatagggaa tatagctgat
      241 aagtttcctg taaatcctaa tggctcactc ataaatatcg ccgctgtgtg caacaaagag
      301 ggaaatgtga tgggaattat gccgcatcct gagagagctt catggatgag gcaggtccct
      361 ggcaggcact tgcttggtga acgggctggt cctggaagga agatatttga gagcataaag
      421 gagtatctaa gatgagcgtc gaattgtttg tgggcttgaa gattcctgac aatacagcta
      481 tcactgcgct tcatgcaata gagaggatgg gctataagat aggcaaatta aagagggaga
      541 attattataa ttttgagata gacggcgatg aaaacagctt tagcttaaag ataggaaagg
      601 tggatatact ggtgaatgcc aacaagaaca aatattctac tgcacttgca agggaagagg
      661 gggctcatca tgtgcttgtc aaagacagcg atgataagtg tgaagggttg ataaaatcat
      721 tgcataaact cgggtttcca gagataaaga gcatgcagag gggtgtgctt tgggcgatat
      781 actctgattc ttcggatact gcaaaagaga tagctgagaa actgctttat aacaggcatt
      841 atcaggagat tgagatttta taataacatt taaaaataag cattcatttt tttctatatg
      901 ttgcttgtaa tcaattatgg ttcgcaggtt tgccatctaa tagcaaggcg tatccgagac
      961 ttcggagtgt attctgagat tgtgccgttt gatgtttctg cttctgaggt agagagatta
     1021 gcgcctcagg ggataatact atcagggggg ccttcttcgg tctatgacaa aaatgcgcca
     1081 atatctgatg ccaagatttt agagcttggt gtgcctgtgc ttggaatatg ctatgggcag
     1141 caattaatcg gaaaacagct tggcgggaag gttatcccaa acaagataaa ggaatatggc
     1201 aggaggatcc ttacaatcga gaataaagac aagctgttca agggacttgg cgataaagaa
     1261 gaagtatgga tgtcccatgg ggatgctgtc gaagaattgc cttctgggtt tatcactctt
     1321 gcatctactg aatcatgtga gaatgcgtct atcgctgact tgaagagaga tatctatggt
     1381 gttcagttcc atcctgaggt aactcatact gtaaagggcg agaagatact tcggaacttt
     1441 gtatttgata tatgcaaggc caagaaagac tttgatatcg gaaagatgtc ctcaaggata
     1501 attgaagaca tcaaaaaaga ggttggagac aaatcggtct tgatggggat ctccggcggt
     1561 gtagattcaa cagttgctgc aagccttata cacaaagcca taggaaagag gcttcattgt
     1621 atacttatag atcatgggct tgtaagaaag gatgaagtta aaacgataaa ggagatgtat
     1681 gataagcttt cgcttaatgt tgaatacgtt gatgcctctt ctgttttttt aggcaagctc
     1741 aagggagtga ctgatccaga acagaagaga aagataatag gacatacgtt tatcgaggtt
     1801 tttgagcgag aggcaaatag gcttgaaagc aaggacaaaa aaatagaata tcttgggatg
     1861 ggaacaatat accctgacag gatagagagc gctcaacctt caaagcaagc tgacaagata
     1921 aaaagccacc ataaccttac actgcctgag aagatgaagc ttaaggtgat cgaacccata
     1981 aatgagttgt ataaggacgg tgtccgtaag cttggcaaag agatagatgt tcctgacgaa
     2041 cttcttatga ggcatccatt tcccgggcct gggcttgcga taaggatact tggagagata
     2101 acttctgaaa gactaaggat acttagagaa gctgatgata tttttatttc tgaacttagg
     2161 aagaatgggc tttatgacaa ggtatggcaa gcgtttgctg cccttcttcc tgttaaggct
     2221 gttggtgtta tgggggatag tcgaacttat gagtatataa tttcgcttag ggctgtgtgc
     2281 tctatagatg cgatgactgc tgactgggca aaattgcctc ctgagttttt agagttagtt
     2341 agcaatagga ttattaatga ggttaaagga gtaaatagag ttctttatga tgtgacccag
     2401 aagcctccgg gcactattga gtatgaatag ttagttggga ctacttggtg ggattccaaa
     2461 ttgctcctct tcccataagg gctctttgca catgttcggt aaaataactt gctagaataa
     2521 gagataaata acaagtatct atccattcaa gattggtctt tgttactgca accaattctt
     2581 ccaagagatc aggtttccct gccaaaacta aaccttagag cgtagtggca gcttttgtat
     2641 agatgatctg ctcactgcct tttccaaccg tgtaatactg ttgagtgtca ttcataacaa
     2701 gcgatttaat taaaatctat gcctgccctt tgctttctcc tgtgacaatc ccacctgcta
     2761 tcaagtgcgc tactcttatc ggctcaggta tcaatgaacg agtgcaggta agtttcaata
     2821 ttgcccttgc ttctgcaagc gacaggcctt ttaattggat gaatatctta ccaatctgat
     2881 ggacagggcc tgctttttta attaatgcaa acttttcctg tttatttatg ttgataaggg
     2941 catcttctat cttcttaaag tccgggtact ttcttataac gacgataact ggaatatttg
     3001 tttttttatt cagttcttct atgtctagta cattaaaacc gcctagcgct atcccattaa
     3061 ttaggatgca tctcaactgc agcttaaatt ttgagaggtt tatcatctta ataatctttt
     3121 gcgttgagtt gctgccgtca actgcaacct ttgtagtaag gatcccttca acaagagagc
     3181 caccgcggaa tattgcgcca actacaagaa cacttccttt cttgaacttg tcgaaaggag
     3241 aatcgtcaat gcctatcgtc cttatctctc tcttgaacac ttttaatgcc cttatgatac
     3301 catgggctaa tgattcacaa tacttataaa tatataaatt atccttaaag ggatgtgtgg
     3361 aatcataggg tatgtaggaa acaacagggc agtcgatgtt cttctgagcg gacttaaaaa
     3421 acttgagtac aggggctatg attctgctgg gattgctaca atcagcgacg gcaagataaa
     3481 aacaaaaaaa gacatcggca agatagaaga ggtaaatcaa aaagttaatt tttctgagct
     3541 ttcaggaaac atagggctgg cccatacgag atgggcgaca tgcggcaagg tcacaaaaga
     3601 aaatgcccat ccacatcttg attgcactgg caaaatctca ataatacata atgggataat
     3661 tgaaaattat gggcaactga aagaagatct gataaaaaaa ggacataatt tttcatctaa
     3721 tactgataca gaagttatcg cacatctgat tgaagatgaa tataaaggtg atctcaagga
     3781 ggcagtcatc agtgcaatta agcaccttga aggctctttt gcacttggag taatccatgc
     3841 cgacaaagaa gagcttgtcg cggcaaggta tgaaagccct ttgataatag gtgtgggtag
     3901 cggagagaac tttatcgcat ctgatgttcc tgccataatg gaatatacca agaatataat
     3961 ctatctggga aacaaagagg tcgctgtgct taggggagac agtgtctctc tgtttgatgc
     4021 agaaggcaat ccagttgaag gaaagataga gaagatagaa tggaacgctg aacaggccca
     4081 aaaaaaagga ttcaagcact ttatgcttaa agagatattc gagcagcctg agacgataac
     4141 tgagacgata aacgagaggg tctctgatac tgagttattg attggcagag agatagggct
     4201 ttctgcaaaa gagataaaaa acatcaaaag gataatcatc atcgcatgcg gtacttcatg
     4261 gcatgctgga atagtaggcg aattcatgat agaagagata gccagaatcc ctgtcgaagt
     4321 agagtatgca tctgaattta gatatagaga tcctatagtc gacaaagata cccttgttat
     4381 ccccatatct caaagcggtg agactgctga cactcttgct gcactgcgcg aggcaaaaaa
     4441 gaaaggtgct aaagtggtgt ccatagtaaa cgtcaagggc agttctattg cccgtgaatc
     4501 agacagcgtc atatatactc gagctggtcc tgagataggc gttgcttcaa caaaggcgtt
     4561 tactgcccag cttacggtac tttatctact gactgtgtat tttggaagcg tgaagggagt
     4621 gcttcctcct gagcaggtga aaaacaggat aagggacgta aaaaaactgc ctttgcagat
     4681 acagtctgtg cttgaagata aggattatat caggatgcac gctgagattt tatatcagaa
     4741 gaaaaacgca atatatcttg gcagagggat aaactatcct atagcccttg aaggagcctt
     4801 gaaactcaag gaggtctcat atatacatgc tgaagggtat cctgctgcag agatgaaaca
     4861 tgggcctatc gcacttatcg acaaggatat gcctgttgtt gtgatagcaa caaaggatga
     4921 taggacttat aaaaaagtgt tgaataacat cgaggaagtc aagacccggg gagggatagt
     4981 cattgctatc gaatccaaag gtgatgaaga ggtcaagaaa atagctgacc ataattttta
     5041 tgtgccacag aattcatata tacttactcc tctacttgca gtaattcctc ttcagcttat
     5101 ggcttattat atcgctgatc ttagaggctg taatcctgat tttcccacca accttgcaaa
     5161 atctgtaact gtcgaataac cactaaatcc atttattttc tatacactag aaagccttaa
     5221 atattcgacc atttttccaa atgtgtatat ttatgaagac ggtaaaattt aaatcatttg
     5281 taataaaagt aaaacataat gggacgtgtt ttattcgact tcctaagaga attgcaaaaa
     5341 aatatcccgc gaagcaagaa ataaaggttc taatcgataa caagattggt ttcttctgca
     5401 atatcagaac atggcgagtg attggttttt atgttcctgc gactattgcc attaaggata
     5461 atctctttga taaggaagta gaagtcgcaa ttgaaaaaat cgatggtttt tacagcacgc
     5521 tattgccgga tggtagggta tacttaccta acaacttagc cattaagcta aagcttagga
     5581 agcaggatat aatcttgctg gaatttaatg ataaaaaaga gtactgtaca ataaatttca
     5641 ggaagaaagg aagtactgaa gagttttttt gtatgtttgc caaacagtct atcaacagac
     5701 agaaaggtgt atttaaaata aaaaagattt ttcggcaaag aaatttttcg gtgctgagtg
     5761 gaaaaatcca tgacgatttt gcgtttgaca actgtgcaca aattaataaa agaagacttt
     5821 tattattcca tggcaatagg atcccattat tagtagatcc atgtattaat atagagaaat
     5881 ttgcgcatta tttaggctgt tactttgcag atggaaataa aaaaggcaat tcttggggaa
     5941 tatgcgcatc tacatttaag caagctattt attataagag tatgcatgaa agattaatca
     6001 aggatgctca gttaacgttt ggaatatcct attctaatgc ttctgatgtt ggcgctgaca
     6061 caataaaaca agagctcata aggaagtggc aggaaaatac atcgattgga ctagagggga
     6121 ttgatgttag aataatcaca ataaaagatg aaaaagtgct aaaaagaaat cagtatggtt
     6181 ctttaatcct aagggaagat agggatttaa ccagattata ttatgtaaga atgatggatt
     6241 tattgctaca caagatcacc gaaacaaaga ataaagcatt agcaattgac tttatacatg
     6301 gtgttttaga aggagatggc tccgttgggg caaagagagg tagcctaata attacgtcca
     6361 atttaacgga tttagaacta cttgaacggg ttatgaaaga aacaaggttt gtttttcata
     6421 aaagaaaaga gggcgctaat aagggattca tatacatagg atctttggaa ataatcagaa
     6481 atatatctat cttaaaggat aatctcttta aattttatcc caaaagaagg aagttattga
     6541 aagtcaggct aggggacact ggctgcgcta gatttttgct gggaaaaagc aagaaaacct
     6601 ccaattggtt gataggacaa ttgaaagaaa aaggaattct tgacaatgac tgcaaattga
     6661 caaaagaagg ccaaaaaata caaaaaaatt taaagcaatt tctattatcg ggggattgat
     6721 atgatgatta gggatgccac ttcaatggat gctcgtggaa ttggggctgt tttgaaggaa
     6781 tgctataata ttgcctctgt tgaagaagga gttttagtat ttaattctga gactgctaaa
     6841 ggccatcact atatcgttgc cgaggataat ggtaagattg tggggttgac aacctggttg
     6901 atgcacggat tatttagaca ccaactttgt gagctggata ggattgcagt tttggctgat
     6961 tatagaggca aaggagctgc caaagcgctg tttgatgccc tcgttgtttc agcagatgct
     7021 ttctataaaa agcacggatt taagcttaga aagttatatc tgttgacgca tgctgacaat
     7081 ctccaagccc aaaggttcta tgaaaagatg ggctttagtc atgagacgac gctgaaaagc
     7141 cactattacg atggaaagga tgagtttgtt tatagccgtt tttttagatc tttcttgtaa
     7201 agcttaagaa accggtatgc actgtctggg tcgttatagg ccttactttt ctatcctcaa
     7261 tctcccaatc cctttgcata agttctgtgg ttttgatgtg gatgaggctg ctgtcttttt
     7321 ttatggctga tataaagtcc atgacctgag ggatagtcgg tgagtatgag acaagaaagc
     7381 cgccatgctt cagggctttt tttgcatgat ctataacctt ccaaggctcg ggcaggtcaa
     7441 gagtgataac atcaatattt ttttcatcga tgccattgta gatatcctga ttttttatct
     7501 ttaggttcgt taggtcaagc atctgcctgt ttttctctgc tatctcggca aagtccggcc
     7561 ttagctcata agaggtcact tgcttgcaga tgtttgcgag aaaacaagat aatgctcctg
     7621 agccagtgcc tgagtcaatg actttcgaga tctttcctat acccgtttct gctatgatca
     7681 tccctatgtc ttttcttgtg attatctgag gggctctttt tatcctggta taaaggtcga
     7741 tgaaggatgg tgagaatagg gtaagctctt ttccggtgtt ggtctttatg attgagccgt
     7801 cttttttggc taaatctgag gtagaaacaa agccgtattg gcaatgatag tctttagaaa
     7861 tgtctttgac aaagaattgg ttcccttctc tggagatcaa tagtttactt gtcataataa
     7921 agcgggcctg gcaggattcg aacccgcgac ttacagctga cttccttctt aatagaaggc
     7981 tgtcactctt tttggccaaa aaccaacaga agactgaggt cttttaggta tccaagctga
     8041 gttacaggcc caacaggctt taagaatgag catttcattt ataaatttaa tctagaaatc
     8101 tactgcatag gcataagctg atctgctgac tcaacctttt gggtgcattg cttcttttct
     8161 tcccaataga cccagcactt ttttccaaac tggtcccatt tcaagcatcc gtcgcagcat
     8221 tttggtgttg ccataaaata tgagaagaga aaggtagtat aaaaaagtta ctctatccaa
     8281 tccttgtttt tcagcttctc aggaagatat tcctcagaga taaagcttat cccgcgagct
     8341 gacaaagcct ggatttctgc ttttgctcca aactgcttca tcttcttgaa ttcatattgc
     8401 cattgcttgc tgttcttgaa ccattcgtag gcagaaatct gctttagccg tgactggtcc
     8461 tgatcggtca atttgatgaa atgcttttgc aagccatact tttcaatatc atcagctttt
     8521 atccctaaat acctgcaatt tggcactgaa agagaatctg ccatatgagc cagacttatc
     8581 gaaccgaatt ttaaaactga gtagatataa tgcccccatg aatcaaaatc tgtgaggaca
     8641 agtatcggta aattatgctc tgtgctcaat ctttgaagaa gccgccttat tccccttgtg
     8701 gtctggccct gtgaagacat gatgatgcag ttttgctttg cccagaactt atcctcatgc
     8761 aatctctccc agactgctgc cttttccatg tagatgacgt attttgcgtc aaccttcttg
     8821 aatttgatat tctcgacgtt gcttggtatg gaccatccgc ctgagcccag ccttgaccag
     8881 tctatctggt cgcccatatc ttcgatgatc acttttcctg caacgcttcc catcttgttt
     8941 gcgttgacgt ggagacattc ccttgaatag ccagttataa tctctaagtc ctctattgcc
     9001 ttgtctgatt ccttctgctc gtcaactatg ttgatattgg tcgagggaat agtccttttt
     9061 gccatgtaaa agacatccct taagcttgca tgcttttgca catccaacaa tgatttacaa
     9121 agagcagcta cttctatcgt ctgcaagaac ttctttgagt ggcctacgtt aaaaaagaac
     9181 ctttgtgctg tcttgtcgcc aagacctaat gtttttgact ctttatcata gactacgttg
     9241 cttaaacccc tgactgaaag ctctatcttg gggttgtttc ctttttctat gtcttttact
     9301 atctctttgc caaggtttac aagttcgctg cctacctcgt cctttgttcc tttctttgag
     9361 tgttctgaca atgacgactg cgattccttt ttcatcttag ctgttatcct cttcatcgct
     9421 gtttttttca agctttgtgt cgtcttcaac ttcgcctttc agggattcca tctgatatat
     9481 ctctttttgt atatcatctt tctttaccat ctcttttagt ttttttagga tggcttcctt
     9541 gttttctcct gtgagcctgg aaagagaatc cgcaacctct ggcatatacc tttcaaacag
     9601 atttgccctt tcaagctggt gctgtacatg cctttttttg tggatatgaa gctgaagctt
     9661 tctgccgcat tcctgcaaag caagctttat ctctttgatt atctccggat aatgagctat
     9721 tgcttccttg ctttctgaag tgaatggtac ccatactgag tttatcgaga ctgctatcgt
     9781 tacagggcct actgggagtg cgcctttgct ttgacctagg ccataattct tccaggctgt
     9841 gccgatggct gactttgtta cagcacaagc tccctgctgg tataataaag ggaccctatt
     9901 tgcatatctt aatagaatgg ctgtgtcttc tgcactttgc tctccaccat aagcgatgcc
     9961 tgcttcaacg atgaatggat tgcccctgta tactgatggc ggcctggtcg tcgcgcaata
    10021 aaattcggca tttatctctt tttgcaggcc tttttcaaga agctcttctc ctatgggcga
    10081 gatacagttt gttggggggg cgattatctt tgtctttgtt ataccctgca atagtttttc
    10141 agcaatatcc cggtttatct cttttggctt gcagcttggc ataaggcttg agttttcaca
    10201 tatctctttt gccgtgcctg cgcctactct tgaaaattcg ctcatcaaaa atgactgaag
    10261 agtccttgat tctgttgctc ccagcatctt tatcaggacg ccaagctcta ccccataagg
    10321 atgaggcatt atctcaacag gttttggagg caaagtgtct gttgccctag caaatatcat
    10381 ctgctctgct ttgggattag tgtaaattat agttgcgtga ggatttacta tcgaagtctg
    10441 cttaagatat tggtcgatag attgaaagcc accttgatag tttccttcga gttcaagctc
    10501 tatccttgtg ccatgctctt tgttccactc tatcacttct tcttttatta tctcgggcat
    10561 gtttttctct gtgtttagct taagctcaag gaataaagca tcgtgttttg gagaaatcct
    10621 tgaagtgatc tttgctggct ttcctgttgt caactgggca tagaggactg atgctgagat
    10681 gccaatgcct tgctgtccac gactctgcga aagcttaaag aattttgaac catagagtaa
    10741 tcgtgcaaaa acatggggga tctgcttttt tacaatgcct gggccgttgt cttcgataac
    10801 tatcctgaag cgatcatttt gcatgtctat tacttcaaca ctgacctctg gcaggatgct
    10861 tgcttcctca caggcatcaa gagagttgtc tacagcttcc tttatagtcg tcatcaatgc
    10921 ttttcttttg ttatcaaagc ctagaaggtg cctgttcctt gcaaagaatt cagctactcc
    10981 tatctctctt tgcttttttg caagctccat tgctattgga gtttcttcag acatattcaa
    11041 aaacctgaag tcaagtggtt attaaaactt tctatttgca ggttagtggt aatttatcct
    11101 gaatttggcc gtaagtgaat tttaaccatg ccttcgacgc tttgctttgc aaggctcagt
    11161 gccacagaaa aattagtttt ctttgttctg ctcgttcaaa aactcaaccg acgctttata
    11221 gcatactcgt cgattatagt ttatcaatca atttatgatt atcactcggg aatcatcccg
    11281 acaggatcct ggcaacggct ttgcaactgt ttagaataaa atgtagattt attccaaatc
    11341 cgcgcgtaac attctttctc tcttcatcat tgtgcgtttc ttttccaaga atttgtaaac
    11401 agtggaatgg gggctgcctc ttaacagctt ttctattgct tcccttgcat tacttgcagc
    11461 agtaggctct cctatcactc ctgcggtttt cccataaaca catatatggg ctcctgtcaa
    11521 ctcttcgatc agccttcttg tctttccttc tgcgcctatt atccttcctt ttatccttag
    11581 aaggtggtct tttgactttc caacataatc ccttaggtcg ataagctcaa aagcataatc
    11641 tcccttcaaa agcagctttg caacctctgg attaaagcct cgaccgatgg ctcttatcac
    11701 ttccctgcta gaatatagcc ccagggcttc ttcgcctgtg ataaaaatat ctccttcctt
    11761 gctgtcgacc tttatagtgg tctgtgtttc agcttctatc tcgcttttta tctttccttc
    11821 ttttcctatc aaaaccgcaa tgcggtcttt aggaatccta agttcgtata ggtattctgt
    11881 catgtgacac ctggaggtga tattttttag ggattcttta tcgtttttaa aactttgcct
    11941 aaatctattt ttaggccaag cttggtaaag taagtggcaa caatcctaac atccctttgc
    12001 agaaactcca tagcccttga gttatccaag atggttgcct gagaaaagtc aatcagcatg
    12061 ggctcttcat catgatttag gatattgaat ggagataaat cggcatgtac caagccggcc
    12121 ttgtatagtt ttcttatatt atccactacc ttatcaaaga attttttcgg gttttttggc
    12181 gcttctacgc ttaaccttga tgatacgttg tctgccttgc ctataaactc cataacaatt
    12241 atgttgttct tgaaggttat cggggtaggt acattaacac ctccctctct tgctttcagg
    12301 agatttcggt attccctttg gacccaggaa aaaattatct tacgcttttt ccccttaaga
    12361 tttgaatatc gtggatcttc ctttatgtaa tcatacatcc ggttaaaatc gcaggcttcc
    12421 aaccgataaa tcttgacaat aacccttgac ctatcctttc ttcttgctga aaaaatgttt
    12481 gcttcctttc ctatcgaaat agggctttct aggccttcaa aatagccttc tgaaataagc
    12541 ttgaagagcg tccaattggt aaattcatcg aagacattgt gcattgtctt gaatttttct
    12601 tttgaacgga taggcattac tgaaagatac actgattcct atttaaagat tgagattaat
    12661 tgtttagagg ctttatacta cttcagcata gttacaaatt agaaagcttt atatattagc
    12721 tctactattt gatggtaaca tagttgaggg tggctaattt ggccaaaaaa gccagataaa
    12781 ggccaggtgt taccaaaatc actgtccaaa cacctctttt ccctgcaaag actgtcggta
    12841 agcttgaata aagcttatgc ggttttaaaa acagcctttg caaggacttt tagcggaaaa
    12901 aggctcataa tcacctcttt ttccctagca agggtctcgc aagagatctt tgctagggtt
    12961 tataatcaca acagtaagta ttcagtcata tataaactaa cttcttagga ctatatgttc
    13021 catcaccgaa atctttattt agttatagta tatattaata tatatcatgc gtaaattcaa
    13081 gttccaagat ggcaaattta cccttgaatc aga
//
LOCUS       DASRUK010000116         6154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_254233, whole genome shotgun sequence.
ACCESSION   DASRUK010000116 DASRUK010000000
VERSION     DASRUK010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 6154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6154
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254233"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VFF28_05175"
     CDS             complement(<1..815)
                     /locus_tag="VFF28_05175"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZX45046.1"
                     /translation="MVNVVIIGLGAGGFGAALAAKKNRAEVSIIDNKSFDLLHQCGLP
                     FALEGKLAFSGLEHPIHAEKMGIKIVRGKVEKIDAGAKHVFIGKDAVKYDKLIIATGS
                     KPFVAPIKTSNKIYTVHNDRDTQELEKNIVEDGKAIVVGAGAIGLETAVALKKKGMDV
                     KVVDMLASTFPKAIDKDMSEIIEAKLMEKGIKLELGKRIDSIEEKAIVVMATGVKPNV
                     DFLEGSMVEINVSGIVVDDKMQTTVKDIYAAGDCCGVKSLINGQRWNSILANNA"
     gene            complement(852..2558)
                     /locus_tag="VFF28_05180"
     CDS             complement(852..2558)
                     /locus_tag="VFF28_05180"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010870687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HZX45047.1"
                     /translation="MVKFIVVAGGVISGVGKGVTTSSIGKILQEYGFKVTAVKIDPYI
                     NFDAGTLRPTEHGEVWVTDDGGEIDQDLGNYERFLGADIPKKNNITTGQVYGKVIENE
                     RAGKYLGETVQFIPHIPDEIKKRIKDAAEGFDFCLVEIGGTIGDYENIPFLFAMKSLE
                     RELGKENVVYVLITYLPVPSHIEEMKTKPTQQAIRLLTEHGIMPDFIICRAKKPLDAI
                     RKKKIELYANIRSDHVISEPDIDTIYRIPLDLEKEGFGTKLLDYVGLKPKAKPDWREW
                     KKLVDNIADNGKPVRVAMVGKYVDIGDFTLTDSYISINQALLHAAANLNAKIEIEWID
                     SKSFEKDESKLSVLKKYNGIIVPGGFGATGVEGKIAAIKYARENNIPFLGLCYGMQLA
                     VVEYARNVCGLKGANTTEVDKGTKYPVIDILPSQEKILAEGKYGGTMRLGAYTAILKE
                     GSKVLALYKERIEDDKKRIARLKDGETFRLGKLEEGKKAILERHRHRFEMNPKFIEET
                     EKKGLVYSGYHIRVDGEKLMEFVELPKHKFFIGTQAHPEFKSSLTKPAPLFYGFVKAA
                     MG"
     gene            2711..3373
                     /locus_tag="VFF28_05185"
     CDS             2711..3373
                     /locus_tag="VFF28_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC59/DGK1/VTE5 family protein"
                     /protein_id="HZX45048.1"
                     /translation="MDWNFKKELGRKAIHLLSIFFLVTYIIFASTFSDKIALLILGFF
                     LIILIEFEYLRVETGLKIPVLSLLWKYKRKKEKNRLGGEVYFLIGSILCLALFDLRIA
                     AAAILMTTFGDMAAALIGKRFGRVGVMKNRTLEGILAELIVDLLIGFLLIRTLVGSSP
                     WWLTSIIPSGTPIWPIIIVMAFTATIVETLVSKLDDNLLIPLFSGFNGQVALILIILA
                     GG"
     gene            3624..3767
                     /locus_tag="VFF28_05190"
     CDS             3624..3767
                     /locus_tag="VFF28_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45049.1"
                     /translation="MNIIEKSKEFYKKIGHKGGLVGGDRVKHLVEKGKKSERGEKHGK
                     GSH"
     gene            3748..3933
                     /locus_tag="VFF28_05195"
     CDS             3748..3933
                     /locus_tag="VFF28_05195"
                     /inference="COORDINATES: protein motif:HMM:NF022829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HZX45050.1"
                     /translation="MARDPIEVQSYLKGIKYPASKKDIIDTAQKNMANETAMDTIRRL
                     PDKPYLSPAHVTKEVMR"
     gene            complement(3934..5382)
                     /locus_tag="VFF28_05200"
     CDS             complement(3934..5382)
                     /locus_tag="VFF28_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45051.1"
                     /translation="MTDERIIGKGRSGIVYLLNPDTVRERLESSGLITVDPVWQEKGD
                     FSGTVEKRFEAHGFLKGAQTLAFGAPVYDNDKDTVLAAYHRRFVLDDLCEYWFEGKLR
                     VAKPRYVQYDQLSKSFALAAEFIKGRAPKILSPFNHDTSELTDLVETTQIFQAHLIEA
                     GFVGTLWQAGYGNPVGLNNYLLDTQSPSQNGNKWVMIDCESGASPYVPIRWKAFRHYV
                     KESLRLKRLLFDDVDIVQLKLYVSKQEGGLQEKLGSERHEILLSNIVKLEEHQSHWLS
                     RTRIERGIAYKQAKGEISARQADWYLNHPLLWYGGQTTKLAMKGSLRLYDKLVGEMPC
                     KLANEVLVTLGEPITFVLSEKHRAEIGRYHASKLITTWQEKGRLSAQQAYDLREQLGA
                     HQLSEYMGDFAGHAWFAFLGIIPKAGNLAYPIQMTASAAMKNQQLPMFLVVGLTSKMG
                     EKIPIIGGSDTLTEHYFNRIGYRVGRYFGKMG"
     gene            complement(5523..>6154)
                     /locus_tag="VFF28_05205"
     CDS             complement(5523..>6154)
                     /locus_tag="VFF28_05205"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZX45052.1"
                     /translation="AGCAAYAEAVVLSPNVPPDMCIPGQKAVALQVAKIVGKEVSAAK
                     AKPIPRLKCTGGSSDKFSYDGIKSCKAASLVSKGPKSCVYGCIGLGDCKEACLFGAVK
                     MENGIPQFNPEKCIACRACAEACPKGLIDMVPGDKQIHVLCNSKDIAKDALKACKDGC
                     IKCKLCEKNCPVQAITVTSGIAQIDYAKCTMCGKCITVCPKKIIVDERK"
BASE COUNT         1666 a         1483 c         1120 g         1885 t
ORIGIN      
        1 gcattgtttg caagaataga attccatctc tggccattta tcaaactctt gacaccacaa
       61 cagtcccctg cagcatagat gtctttgaca gtggtttgca tcttatcatc taccacaatc
      121 ccgcttacat ttatctcaac catgctgcct tcaagaaaat caacattcgg cttaacacca
      181 gtcgccataa ccactatcgc cttttcttcg atagaatcta tcctttttcc aagctctaac
      241 ttaatgccct tctccatcag ctttgcttcg attatctcag acatatcctt gtctattgcc
      301 tttggaaaag tagaagcaag catatcgacc acttttacat ccatcccttt cttctttaaa
      361 gcaacagcag tctcaaggcc tatagcacct gcaccgacaa caattgcctt accatcttca
      421 acgatatttt tctcaagctc ttgggtatct ctatcgttat gaacagtata tatcttgttt
      481 gacgtcttga ttggagcaac aaatggtttt gatccggtcg caattatcaa tttgtcatac
      541 ttgacagcat ctttgcctat aaacacatgt ttagcgccag cgtctatctt ctctaccttg
      601 cctcttacta tctttatgcc catcttctca gcatgtatcg gatgctcaag gccagaaaat
      661 gccagttttc cctccaaggc aaaaggaagc ccgcactgat gcagcaggtc aaaagacttg
      721 ttatcgatta tgcttacttc tgctctattt tttttggcag caagcgctgc accaaacccg
      781 ccggccccaa gcccgattat cactacattt accatagaca gagaaaatta gataatcgaa
      841 tttaaatgtt tctatcccat tgcagctttc acaaatccat agaataatgg cgctggtttt
      901 gtaagtgagc tcttaaactc aggatgagcc tgtgttccga taaagaactt atgcttcggc
      961 agctctacaa attccatcag cttttcgcca tcaaccctta tatgataacc tgaatagaca
     1021 agccctttct tttcagtctc ttcgatgaat tttggattca tctcaaacct atgcctgtgc
     1081 ctctcaagga tggctttctt tccttcttct aattttccaa gcctgaaagt ttcaccatcc
     1141 ttaagccttg cgatcctttt tttatcatct tctatccttt ccttatacaa agcaagcact
     1201 ttgcttcctt ccttcaggat tgcagtatat gcgccaagcc tcatggtccc gccatacttt
     1261 ccctcagcaa ggatcttttc ctgtgaagga aggatatcga tgacaggata tttagtgcct
     1321 ttatcaactt cagtggtatt tgctcctttt aggccacaga catttcttgc atactcaaca
     1381 acagcaagct gcattccata gcataatcct aaaaatggga tgttgttttc cctagcatac
     1441 tttatcgctg ctatttttcc ttcaacacct gtcgctccaa acccgcccgg aactattata
     1501 ccattatatt tcttaagaac agacagcttg ctctcatcct tttcaaaaga ttttgagtca
     1561 atccattcaa tctcgatctt tgcattaagg tttgctgctg catgaagcag tgcctgattt
     1621 attgatatat atgaatcagt caatgtaaaa tctcctatat ccacatactt tccgaccatt
     1681 gcaaccctta caggcttgcc attatcagcg atattatcga ctagcttctt ccattctctc
     1741 cagtcaggct ttgcttttgg ctttagccct acataatcca gaagttttgt cccaaaccct
     1801 tctttttcaa ggtcaagtgg gatcctgtat attgtgtcga tatccggctc agagatgaca
     1861 tgatctgacc tgatgttcgc atacagctca atctttttct ttcttattgc atccagtggc
     1921 ttttttgccc tgcaaattat aaagtcaggc attatgccat gctctgtgag tagcctaatt
     1981 gcctgctgtg taggttttgt cttcatctct tcgatatggg aaggcacagg aagataggtt
     2041 atcaggacat agacaacatt ctcttttcca agctccctct caaggctctt catcgcaaaa
     2101 agaaatggaa tgttctcata gtcgcctatc gtcccgccaa tttccacaag gcagaaatca
     2161 aatccctcag cagcatcttt tatccttttc tttatctcgt cagggatgtg agggatgaac
     2221 tggactgttt ctcccagata ctttccagct ctttcattct caataacctt gccatatacc
     2281 tggccagtcg ttatattatt ctttttagga atatcagcac caaggaatct ttcatagttg
     2341 ccaagatcct ggtctatctc tccgccatca tcagtgaccc atacttcacc atgctctgta
     2401 ggccgaagcg ttccagcatc aaaattgata tacggatcga tttttacagc agtaacttta
     2461 aacccatatt cctgcaatat ctttccaata gaagaagttg taactccctt tcccacaccg
     2521 cttataactc cgcctgcaac aacaataaat ttaaccatgg atactcacct ctgatagact
     2581 tccagaaaca gcgataatat gtagccgcat tacattacaa acacaagctg gtttataaat
     2641 ttaacgcttg cgcctgccca ggtcaggacc acccaataat tttaaatact cctcagccaa
     2701 taagcactct atggattgga acttcaaaaa agagcttggg cgcaaagcaa tccatctctt
     2761 atcgattttt ttccttgtga cctacatcat ctttgcctcc acttttagcg ataagatagc
     2821 actgctcata ctgggctttt tccttataat cctgatagag tttgaatacc taagagtaga
     2881 aaccggactg aagataccag tactctctct gctctggaaa tacaagagaa aaaaagagaa
     2941 aaacaggctc ggaggagaag tatattttct cataggctca atcctatgct tagcgctttt
     3001 tgacctaagg attgcagcag cagctatcct tatgaccact tttggagata tggcagctgc
     3061 cctgatcgga aaaagatttg gcagggttgg ggtaatgaag aataggacgc ttgaaggaat
     3121 acttgcagag cttatcgtcg accttctcat cggcttcctg ctgataagga cgctggtagg
     3181 tagttcaccc tggtggctca cttcgataat cccatcaggc actcctatat ggcctataat
     3241 aatagtcatg gcgtttactg cgacgatagt tgagaccttg gtaagcaagc ttgacgataa
     3301 cctgttgatt ccgcttttct caggcttcaa cggccaggta gcattgattc tgattatact
     3361 cgcaggagga taagatggac ctaaacgcga ctataccctt gatgcagcct taggcaaatt
     3421 tcctcatgca gaagtgtttg taatcggctt gtttggagta attgcatttg tgtatttact
     3481 ttccgcaagc aggcagatga aaaagttatt tgacgagata gcagaggtca agaaaaagat
     3541 agcaagcctc gagaaaaagc gttcaaagga ctagagaaac tatattaggc aaaaaaacat
     3601 gctatatttt tctggtgatg cttatgaaca ttattgagaa gagtaaagaa ttctataaaa
     3661 agataggcca taaaggcggt cttgtcggag gagacagggt aaagcatctt gtcgaaaaag
     3721 gaaaaaagtc agagagaggt gagaagcatg gcaagggatc ccattgaagt acagagttat
     3781 cttaagggca taaaatatcc tgcttcaaaa aaagatatta tagatacagc gcagaaaaat
     3841 atggcaaacg agacagcgat ggacacgatt aggaggcttc cagacaagcc atacctttct
     3901 ccagctcatg taacaaaaga agtgatgaga tgattagccc atcttcccaa agtatctgcc
     3961 cactctatat cctatgcgat tgaaataatg ttctgtcaag gtatcgctgc ctccgatgat
     4021 aggtatcttc tctcccattt ttgaggttag gccgactact aagaacatag gcagctgttg
     4081 gttcttcata gcagcagatg cagtcatttg gataggatat gccagattgc ccgctttggg
     4141 aataatccct aaaaatgcaa accaggcatg tcctgcaaaa tcacccatat actcagatag
     4201 ttgatgggca cctaattgtt cccttaaatc ataagcttgc tgtgcgctca atcttccttt
     4261 ttcttgccag gtggttatta gctttgatgc atgatatctt ccaatttcag ctctatgttt
     4321 ctcagatagg acaaatgtta ttggctctcc caatgtcaca agaacttcat ttgcaagctt
     4381 gcagggcatt tcgccaacta atttatcata taaccttaat gatcccttca tggctagttt
     4441 tgttgtttgc ccaccatacc ataataatgg atgatttaaa taccaatctg cttgcctggc
     4501 gctgatttct ccttttgcct gcttgtaagc tatccctctt tcaatccttg ttcttgacag
     4561 ccaatggctc tgatgctctt caagtttcac tatgttcgat aacaagattt catgacgttc
     4621 tgatccaagc ttttcttgca atccgccttc ttgttttgac acatatagtt ttaactggac
     4681 aatgtccaca tcatcaaata agagcctctt caaccgtaat gactctttta catagtgccg
     4741 gaatgctttc catctgatag gtacataagg agaagcgccg ctctcgcaat cgatcatgac
     4801 ccatttattg ccattttgac tagggctttg cgtgtcaaga agataattat tgagtcctac
     4861 cggattgcca taccctgctt gccataaggt tccaacaaac cctgcttcta taaggtgtgc
     4921 ctgaaatatt tgggtagtct ctaccagatc ggttagttcg ctggtgtcgt ggttaaaagg
     4981 agataatatc tttggcgctc tacctttgat aaattctgca gcgagagcaa aagacttgct
     5041 gagttgatca tactgcacat atcttggctt cgcaaccctg agttttcctt caaaccagta
     5101 ctcgcaaaga tcatccagta caaatcttcg atgatacgcc gccagaacgg tatctttgtc
     5161 attgtcatac accggagctc caaaagctaa tgtttgggct cccttcaaaa acccgtgagc
     5221 ttcaaacctc ttctcaacag tccctgaaaa gtcccctttt tcttgccaca cagggtctac
     5281 tgttattagt cccgatgatt caagcctttc tcttacagta tcaggattta ataaatagac
     5341 aatgcccgat ctaccctttc caattattct ttcatctgtc attcaaacct atcaaagaac
     5401 ctcttttaag ctagcgattg gttaatcaca tttaaagata ttgattacaa taaaagattt
     5461 caccatatga ctgcctcttc attaatcctc tgtctagaca cctttgcatg ataaagatgc
     5521 ctctatttcc tttcatcgac aattatcttc ttggggcaga ctgtgatgca ttttccgcac
     5581 attgtgcatt ttgcataatc tatctgcgca attccagaag tcacagttat cgcctgcaca
     5641 gggcagttct tttcgcacag tttgcacttg atgcagccat ctttgcaggc tttaagggca
     5701 tctttggcga tatccttgga attgcataag acatggatct gcttgtcccc tggcaccata
     5761 tcgataagtc ctttggggca agcctcagcg catgccctgc aggcgatgca tttctctgga
     5821 ttgaattgag ggatgccatt ctccatcttg acagcaccaa acaggcacgc ttccttgcag
     5881 tctccaagac cgatgcatcc atagacgcat gatttaggcc cttttgaaac aagagaggct
     5941 gccttgcagc tctttatccc atcatatgaa aatttatccg atgagccacc ggtgcattta
     6001 agccggggga ttggcttagc tttggctgca gaaacttctt tacccactat cttagcgact
     6061 tgcagagcaa cagctttctg tccaggtatg cacatatcag gagggacgtt tggactcaaa
     6121 acaacagcct cagcataagc cgcacaccct gcat
//
LOCUS       DASRUK010000117         4549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2104634, whole genome shotgun sequence.
ACCESSION   DASRUK010000117 DASRUK010000000
VERSION     DASRUK010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2104634"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VFF28_05210"
     CDS             <1..369
                     /locus_tag="VFF28_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013984980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HZX45053.1"
                     /translation="AEEVAALKKANLSKLAAEYRVPLKLLEEVRKLGRLPVVNFAAGG
                     IATPADAALCMQLGVDGVFVGSGIFKSTDPKSRAKAIVEATCHFNDAKIIAKASEGIG
                     EAMKGLEIAKLEVRMQERGW"
     gene            370..936
                     /gene="pdxT"
                     /locus_tag="VFF28_05215"
     CDS             370..936
                     /gene="pdxT"
                     /locus_tag="VFF28_05215"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HZX45054.1"
                     /translation="MRIGVLALQGSVSEHIQILKRCGVTPIEVRLPEDLKHVDGLVIP
                     GGESTTIGKLMERFWLDIEIKRKYEQGMPIYGTCAGAILLAKHIVGSTQARLGLMDIT
                     VKRNDYGRQVDSFEADLTIQDKPFKGIFIRAPIIKAHKGCKVLAEYNKHPVLVQQKNL
                     LAGTFHPELTNDTLIHEYFISMVKNKSF"
     gene            1039..3348
                     /locus_tag="VFF28_05220"
     CDS             1039..3348
                     /locus_tag="VFF28_05220"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HZX45055.1"
                     /translation="MNKQIFLQTLKEKILILDGAMGTMLQKNGFTKGCPDELNLTHPG
                     LVKKIHKEYADAGSNLLLTNTFGANSIKLKSYHLQDKVSLINKAAVSNARQACPNCII
                     VGDIGPLGELIEPMGKLTFDEAYAAFKEQAASLKEADAIIIETISDIKELKAAIIAAK
                     EVFSGMIISSMTIQNGRTSTGTDTETYVTVAEALGVDIIGVNCSDGPEGMYETAKIIA
                     KNTSKPICLQPNAGMPRIVSNKTVWDYPIERFADFAKKYQELGANIIGGCCGTNPDYI
                     KAAAKKLKNQKPIERSSQKETRLSSRTKILTIKPTLIVGERINPTNRPQFSSELKQNK
                     TSYIKNQAIQQLEEGASLLDINVGVAGIDEKVTLPLAVAAAQNVANVPLVIDTSNPQA
                     LEEALKKCDGKPLINSVNGSEKSLKEILPLAKKYGAGIIALCLDDEGIPRTKEKRIDI
                     AKRIIQKAQSIGIAKEDIIIDSLVLTIATNPENESIILDTLKEIKALGYKTILGVSNI
                     SHGLPNRSEINSKFLTKAKKAGLDLAILNPIDNILQDNTDLTIKIKKAERADYPNLSP
                     KDQLKNAILYGDRENITDIINNALKTLTALQINDILISALNIVGEKFNKKEYFLPNVL
                     LSADAMKLAFQRLKKELAKEGGKEKGTIIFATVENDIHDIGKNIVIALLESHNYKIVD
                     LGANVKTKKIIEEVKKQKPDLLALSALMTTTVMEMENVIKELRAHGINIPVIIGGAVV
                     TDDYAEQIKAAYGRDALSAVKKINELIKH"
     gene            3356..4249
                     /locus_tag="VFF28_05225"
     CDS             3356..4249
                     /locus_tag="VFF28_05225"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HZX45056.1"
                     /translation="MKKITDLLKEKKFTVSVELVPPRNGEGKDEILNKIENLKGKVDF
                     VSVTKGAGGSLRGGTLPISVFAKEHGIIPISHFVCRERTSAEIENDLIDMYYFDINNI
                     LALRGDPPAGAKDEVWDGDYEYAYLLVNQLKRLNHGKYLPRKNVDTEDSRPGIKTDFC
                     IIVAGHPEDPVEKEIEHLKAKIEAGAEVIITQMIFSFKEYEVYVKNLKKHGITLPVIP
                     GIRPITSMSQADSIERFFGLKVGDELKQGLGGKCEKEAYDFGLNYTAGLIKKLKAFNA
                     PGVHLFVLNDTKIVEDILRLI"
     gene            complement(4236..>4549)
                     /locus_tag="VFF28_05230"
     CDS             complement(4236..>4549)
                     /locus_tag="VFF28_05230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00269 family protein"
                     /protein_id="HZX45057.1"
                     /translation="IKPLYFCTEEEVKLFSKLNKFPVKYEKCPCRADAYRKDVCDMLN
                     VFEKEHKSTKTAIIQSFLKLSPELKKINYGKAKACEKCGEPSSGHICGTCKLIEKIRS
                     T"
BASE COUNT         1610 a          928 c          973 g         1038 t
ORIGIN      
        1 gccgaagagg tcgcagcatt aaaaaaagca aacctttcaa agcttgcagc tgaatatagg
       61 gttccattaa aacttttaga ggaagtgagg aaattaggca ggcttcctgt tgtaaacttt
      121 gcagcaggag gaatagcaac ccctgctgat gcagcattat gcatgcagct tggagtagat
      181 ggagttttcg tcggctcagg tatcttcaag agcacagatc caaaaagcag ggcaaaagcg
      241 atagttgaag ccacatgcca ctttaacgat gcaaagataa ttgcaaaggc atcagaaggc
      301 atcggcgagg ctatgaaagg ccttgagatt gcaaagcttg aagtcaggat gcaggaaagg
      361 ggatggtaag tgaggattgg agttcttgcc cttcagggct ctgttagcga gcatatccag
      421 attctaaaga gatgcggagt cactcctata gaagtaaggc ttccagaaga cctaaaacat
      481 gttgatggat tggtaatccc tggtggagaa tccaccacta ttggaaagct catggaaagg
      541 ttttggcttg atatcgagat caagcgaaaa tacgagcaag gcatgccgat atatggtacc
      601 tgtgcaggag ctatccttct tgctaaacat atcgtcggaa gcactcaagc caggcttggt
      661 ctgatggaca tcactgtcaa aaggaatgat tatggaaggc aggtcgacag ctttgaagca
      721 gacctgacca ttcaggacaa gccctttaaa gggatcttta tcagagcccc catcatcaaa
      781 gctcataaag gctgcaaagt ccttgcagag tacaataagc acccagtctt agtccagcaa
      841 aagaaccttc tggcaggcac ctttcaccca gagctgacaa acgataccct catccatgaa
      901 tactttatca gcatggtcaa gaataagtct ttctagttct tattcctaaa agaatattaa
      961 tatcaacaat gtttttaaag aatagccatg catggtttat agtcagtata agagctatcc
     1021 gatattacaa ataatgcaat gaacaaacaa atatttttac aaacactgaa agaaaaaatc
     1081 cttatcttag acggagccat gggtaccatg ctccaaaaaa atggttttac caaaggctgc
     1141 cctgatgaat tgaacctgac gcatccagga ttggtaaaga agatccacaa ggagtatgca
     1201 gatgcaggct caaacctatt attgacaaac acctttggtg caaatagtat caagctaaaa
     1261 agctaccact tgcaggataa agtcagcctg atcaacaaag cagcagtaag caatgcaaga
     1321 caagcctgcc caaattgcat aatagttgga gacatagggc ctctcggaga attgattgag
     1381 cctatgggaa agttgacttt tgatgaagca tacgctgcat ttaaagagca agcagcctct
     1441 ctcaaagaag cagatgcgat tatcatagaa accatatcag atatcaaaga actcaaggca
     1501 gcaataattg cggcaaaaga ggtattctca ggaatgatca tcagctctat gacaatacaa
     1561 aatggccgca cttcaacagg aacagacaca gaaacttatg taactgtcgc agaagccctg
     1621 ggcgttgaca taatcggtgt aaactgttca gacggtcccg aagggatgta tgaaacagca
     1681 aagattatag caaaaaacac tagtaagcca atatgccttc agccaaacgc aggtatgcca
     1741 aggattgtca gcaataaaac agtctgggat taccctatag aaagatttgc agattttgca
     1801 aaaaaatatc aggagcttgg agcaaatatt atcggaggct gctgcggcac aaacccagat
     1861 tatattaaag cagcagcaaa aaagctgaaa aaccaaaagc cgatagaaag aagcagccaa
     1921 aaagagacca ggctatcctc aagaacaaag atccttacta ttaagccaac tttgatagtt
     1981 ggagaaagga taaatcctac aaacaggccg caattttcaa gcgaactaaa gcaaaataag
     2041 acttcttaca ttaaaaacca ggccattcag cagcttgaag aaggagcatc ccttcttgat
     2101 ataaacgtgg gcgtcgcagg tatagatgag aaagttacac tcccattagc agtcgcagca
     2161 gcacaaaacg ttgcaaacgt cccattagta attgacactt caaacccgca agcattggaa
     2221 gaagccctga agaaatgcga tggaaaaccc ttgatcaact cagtcaacgg ctcagaaaaa
     2281 agcctaaaag aaatccttcc gttggcaaaa aaatatggcg caggaatcat cgcactatgc
     2341 cttgacgacg aagggattcc aaggacaaaa gaaaaacgca tagatattgc caagagaata
     2401 atacaaaaag cgcaatcaat agggatagca aaagaagata tcatcattga ctcgcttgtc
     2461 ttgaccatag cgacaaatcc tgaaaacgaa agcattatct tagatacatt aaaagaaatt
     2521 aaagccctgg gttataagac aatcctgggc gtaagcaaca tatcccatgg tcttccaaac
     2581 aggagcgaga taaactctaa gttcttgacc aaggcaaaga aagcaggcct tgatttggct
     2641 attctaaacc ccatcgacaa cattttgcag gacaacactg acctcacaat aaagataaag
     2701 aaagcagaga gggcagacta ccccaaccta tcaccaaaag accagttaaa aaacgctatc
     2761 ttatacggag atagagaaaa catcacagat atcatcaaca atgccctaaa gacactgact
     2821 gccttgcaga ttaacgatat cctgataagc gccttaaaca ttgtcggtga aaaattcaac
     2881 aagaaagaat actttcttcc aaacgtcctg ctttcagctg acgccatgaa gcttgcattc
     2941 cagaggttga agaaagagct ggcaaaggaa ggtggaaaag aaaaagggac gataatattt
     3001 gcaacagtcg aaaacgacat acatgacata ggaaaaaaca tagtgatcgc cctattagaa
     3061 agccataatt acaagattgt tgacctggga gcaaacgtaa agacaaagaa gataatagag
     3121 gaagtaaaaa agcaaaaacc cgatctcctg gcgttatcag cattgatgac aaccacagtt
     3181 atggagatgg aaaatgttat aaaggagtta agagcccacg gcatcaacat acccgtcatt
     3241 ataggcggcg cagtggtcac tgatgattat gcagagcaga taaaagcagc atacggaaga
     3301 gacgctctaa gcgcagtaaa aaagatcaat gagttgatca aacactaatt ataccatgaa
     3361 aaaaataacc gatctcttaa aggaaaagaa attcactgtt tctgtagagc ttgtcccgcc
     3421 aagaaacggt gaaggaaaag atgagatcct aaataagata gaaaacctta agggaaaagt
     3481 tgactttgta agcgtcacaa aaggtgcagg cggctcttta agaggcggca ctcttcctat
     3541 ctcagtcttt gcaaaagagc atggaattat tcccatttct cattttgttt gcagggaaag
     3601 gacaagtgca gagattgaaa acgacctgat tgacatgtat tatttcgaca tcaacaacat
     3661 tcttgcccta agaggagatc ctcctgcagg ggcaaaagac gaagtatggg acggagatta
     3721 tgaatacgct tacttgctag tcaatcagtt gaaaagattg aatcacggca aatacctgcc
     3781 aagaaaaaat gtggacacag aagactcaag gccaggcatc aagacagatt tctgtatcat
     3841 tgtagcaggc catcctgaag accctgtaga aaaagagata gagcatttga aggcaaagat
     3901 tgaagcaggc gcagaagtta taatcactca gatgatcttt tctttcaagg aatatgaggt
     3961 atatgttaaa aacttaaaaa aacatgggat cactctccct gttatcccag gcatcaggcc
     4021 cataacctcg atgtcacagg cagactcgat agagagattc ttcggtctga aagttggtga
     4081 tgagttaaaa caaggcctag gtggtaaatg cgaaaaagag gcttacgatt ttggcctaaa
     4141 ttacacagca ggcctgatca agaagctaaa agcattcaat gctccaggcg tccatctgtt
     4201 cgtcctaaac gacacaaaaa tcgtggaaga cattctcagg ttgatctgat cttctctata
     4261 agtttgcatg tgccgcagat atggccggaa gagggctcac cgcacttttc acaagccttt
     4321 gcctttccat aatttatctt ttttagctca ggagatagct ttaggaaaga ctggattatc
     4381 gcagtctttg ttgacttatg ttctttttca aaaacattca gcatgtcgca tacatctttt
     4441 ctgtaagcat ccgccctgca tgggcacttc tcatacttca caggaaactt gttcaattta
     4501 gagaacaatt ttacctcttc ctcagtgcag aaataaagcg gctttatcc
//
LOCUS       DASRUK010000118        10800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_921791, whole genome shotgun sequence.
ACCESSION   DASRUK010000118 DASRUK010000000
VERSION     DASRUK010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 10800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10800
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921791"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="VFF28_05235"
     CDS             <1..934
                     /locus_tag="VFF28_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45058.1"
                     /translation="AMQQARRFGSLGPEIYDSLIVAFPYTNLDDSKDINVSMPYLYDN
                     DWNVIWNTTANGTSGITTNLTDYAGYNETWFSGINCSRTDKSANCFVNTTLDVIWIRL
                     PHFSGVGPSASGTAAASSTSTTSSGGGGGSSSAQVYSVSLGNTLSSVGLKKNDQAKFV
                     FGSQTHYITALSISSTQAKIRIQSTPVEILMQVGQEAKVNLDGDNYDDLYIKFDSSSG
                     TKATFSVKKIREAIASVPSAPATKPAEKAQPATTQQGQAQTTPPQVEAKEEVPPQAII
                     PLKKTTYGWIYGALAAALVLLIALSLIIRKVRNS"
     gene            complement(921..1154)
                     /locus_tag="VFF28_05240"
     CDS             complement(921..1154)
                     /locus_tag="VFF28_05240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04076 family protein"
                     /protein_id="HZX45059.1"
                     /translation="MVEIKVISVKGKCEYGHRKGHVYEYPYTGLCPYALHSLWPFITA
                     KQFGGKIPWEKKGKIKVSCPNPDDLVVFEISCS"
     gene            1273..1821
                     /locus_tag="VFF28_05245"
     CDS             1273..1821
                     /locus_tag="VFF28_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015323972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HZX45060.1"
                     /translation="MLKDFIIPFIAIGIAELGDKTQFAIFCLSGKTKKYLSLFLGILL
                     AFILADGLAVILGNLISGIVPGVYIRILSGILFIAFGVFALINAEKDEEKLELKTPFL
                     SAFTIIFVSELGDKTQVASGLFAASYSPVWVFFGVIAALTALSIIAISLGRYIFSRLN
                     PRIISTVSGILFISIGIFSLFR"
     gene            1853..2512
                     /locus_tag="VFF28_05250"
     CDS             1853..2512
                     /locus_tag="VFF28_05250"
                     /inference="COORDINATES: protein motif:HMM:NF025015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HZX45061.1"
                     /translation="MQLKKEMPLMAFLLLMFISSTVLYRYVPEKIPTHWNAKGEIDGY
                     SGKFLGLFLLPIIASILYILFLVLPAIMPYRNNIEKIEGKFYWLKAIMLAFFAVLYLA
                     SILSSFGIQINIIYVMMPSLAVIFYYIGYLMKYTKRNFFIGIRTPWTLVSDKSWDMTH
                     KKASVLFRLYGLLIMTTLFFPSYAFLFLLISLIPLILYLVVYSYLIYRGEKKKAGRSF
                     R"
     gene            2665..2829
                     /locus_tag="VFF28_05255"
                     /pseudo
     CDS             2665..2829
                     /locus_tag="VFF28_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018194239.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
     gene            complement(2812..4482)
                     /locus_tag="VFF28_05260"
     CDS             complement(2812..4482)
                     /locus_tag="VFF28_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45062.1"
                     /translation="MDFQEIAERNGFSRERLKEIYWQVVQEGMPDANHPDMIDVKVKE
                     VLLDSPAKEELFIRCNNQFEAALKHTLVCVAIAKDPEAYERMFRESPYKVLGEEGAYT
                     PHVSAVTRWPGFEFRNRVLKEAGATLQPSFGDAKKSFDGIVVTAENAHLVSEDMIGGA
                     LDFWGCIITNGGSVLTYDVGGLYAGPLMAGKLNAGYRFADHEFAEHMQIFLIESRKLG
                     EILKALKKGIGAIAKRNKGVLVHLEDIKGIDLMETALPIMKGEKNIYVFSDDQLYTGL
                     MATAPLGLFLRKYFSQGKIGIINGLGAAGEGVRKALMHVGLDVPVICTDSRGVIHKGR
                     PDSASLPKFKQEAAIDLRAYGIDSAREVYLGEVLGKIPADKIAFFYETSGRGKYFRDN
                     PQLLKPLSDRSSPIFFALLSNPAVESSDAMRQVLSFFDLDKVSLVSGSPPELIYNAIS
                     DKKGLCDYPHFKKLSHPAQANNIIFFPAAACTIGRLVIDGKIDCTDRDEIRRAHNAAY
                     DGIARMTAGEGLLAPVDQLLVPGGYFDSIRRSILKEFNHMEKVSVEQF"
     gene            complement(4590..4973)
                     /locus_tag="VFF28_05265"
     CDS             complement(4590..4973)
                     /locus_tag="VFF28_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45063.1"
                     /translation="MTNAKDLKPGNYIKINNEILKVTRKEIANCGTHCHSKTKLYVQG
                     LYSKGEKTMNLNHQDSVDQIDITRKEGQVISKMPNKVQLMDQISFETLDADCDEELLS
                     KVFEGDTVTFITFEGMSKVIEKRGV"
     gene            5084..6274
                     /locus_tag="VFF28_05270"
     CDS             5084..6274
                     /locus_tag="VFF28_05270"
                     /inference="COORDINATES: protein motif:HMM:NF042839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6786 family protein"
                     /protein_id="HZX45064.1"
                     /translation="MGRFFAESLDLAKVVERAINTQLKHLVLGPPGGGQVLVIPSLGG
                     RILDTTTAGLEEEGVSFVNEDAMHKGTGGYLNPGNFGGLDVLRWGPEALCCFYSGTPR
                     TVDRYWTPRQWDFSRFFIASQLKPQSPVSSVKMQSTLPIQWVNYFREKISMSVTKRID
                     LCLNPLRDDDRLNSFFNEPGHYVGYRVTVEATNVGNRPLSIDGRNAVALLAVAQFDAA
                     PQSNWIVPITRARDAPLLWDCSQSIHSRQPQAQLTDDYLSVPTKSDTRAIWGVPLGRY
                     CQRVAYYDPSRGELVIFIYNIRDRQADFLKQAWNCTPTERGTLAESLLCPHKPVVGLN
                     YGHRSESGNAAWPCGISLYGPYMGIPVNGSVVLTYHCLRYKGPEETLRKIAADKALLG
                     VTIK"
     gene            6345..7736
                     /locus_tag="VFF28_05275"
     CDS             6345..7736
                     /locus_tag="VFF28_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45065.1"
                     /translation="MSNPDDNLGRIVGDIANILFGLENNNADHAKRWLYDAWQMIMTG
                     AVTPVTQAYIDRCSEGGFAMHHDNKSLFVKKLPNQQAAEQQYAFARRYSSAGNYLMMK
                     AETPFIYQDKGLVLYLVHFARGMDLGAFFLEASKKRSADNFVKQVTEKCIDDLAFWQG
                     DAGNAMAARPAAPEDILGTMKENTLRSVRNAGIYGGISISELDLVLLERSLKIFDGLT
                     FNQHTIVVGSDYCPKNMGIKTDSDKDDLASVMNVMSSNGVDPDPFEIATLIYHWDPSA
                     RWTHWLEDLAAFTTSYEFHNANRKTRLSDWLHKLCSARSSLDPSFKGWIAEQEPSRVH
                     QELSLMILYRALRKADLTATLYAWRNENIGQKICPADYTSVSRSLDSQLSGYSATVGK
                     AVRWLSEGYGPGRIYTPSTDKEAASIFSQYGAEIPVPVCLGFFNYLAQKLRPWTISYP
                     ALKQVKNPSEFAR"
     gene            7733..9712
                     /locus_tag="VFF28_05280"
     CDS             7733..9712
                     /locus_tag="VFF28_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45066.1"
                     /translation="MTAMLEKSNDHVHTWYSDGELTELELILLGLTQGSQRITFTDHD
                     STLAWAPETIAKLNRFFHETLKVSKDGKTSAPCPLFRPYYLQYIMDAARKGDFYFHDY
                     HKIVEIEPGFLKCGGLTLGNGAELTTKLGQTFHILGLGMQQVPSYLSERLDKIAKQRR
                     NRAKEILEFLYRDDYFNKRTLQYLKASQGIIPAGVDEALKRQPVIIDDVLAVNPNAPS
                     RLTIGFLLSQRYGQDVYGNLTPEQVRDIYLQREMIKSQFKIPSFSPKEQIKAILDSGG
                     SSGSVIPVLAHPDEGRRQGWLEGNFGALEALGYSVEKMLEEGAIDESDVRYAAKRSLM
                     ALFIEWGIRGVESAEYARQYGIMDFSHDTDYHGPRMKATRPITKGQGPSLKSLLNIGV
                     VQEAHNHLYWKIRAASEYHAWQEANIITTSLIDRFIQSGTSTQDQSQIITAIPKELTR
                     RPTLAEYLSTFIYSHNDYYTGTVLDIDTKIKQKLEQAAQRGRFVKDSSVNEQIIHYLD
                     ENVDVCSRKKLLVEYVPQLHNIGKVLSFGIEETPEGRYRTTFVQHDKIGAELIDKIRD
                     VLMQDLGLTNKEIDYIRDIATDHVRVHESIYNVMGGIDVEQELDTLKSNVTLDCLLVA
                     KAAGTMSYSGPQDCHNKYFAAANNLIKACTQRIRR"
     gene            complement(9702..10628)
                     /locus_tag="VFF28_05285"
     CDS             complement(9702..10628)
                     /locus_tag="VFF28_05285"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HZX45067.1"
                     /translation="MKRKVIQIADSTQLVSLPRKWALLHGIKKGDEVDIREEGGRLLI
                     TTEDSFSPSRVEINTEGLGIMISRYIHALYKKGVDEIYITYKTQADMQAIQNALGKEA
                     VGFEITNQGNNFCEIKHVSGELEDFDPILKRTFLLLLSMSEQLVDALKNNNYSMLPNI
                     AFLEEANNRFTTTCRRLLNKGGTTPYSPIGPVYYILEDVENLADQYKYLCNQLHDLWK
                     KNKLGRGLGKESIKSLEKTDMTLREFFDFFYKFDKNKFVDIAKKRKELINDLFQIIEK
                     SDKYADRLTAHYLLVITQKIFCLTGPCVVINV"
BASE COUNT         3048 a         2382 c         2266 g         3104 t
ORIGIN      
        1 tgcaatgcag caggcaagaa ggtttggaag cctcgggcca gagatatatg acagccttat
       61 agtggcattt ccctatacaa accttgatga ctcaaaagac ataaacgttt cgatgcctta
      121 cttgtatgac aatgactgga atgttatatg gaatacaaca gctaacggga cctcaggtat
      181 aactacaaat ctaacagatt acgcaggata caacgaaaca tggttctcag gaatcaactg
      241 cagcaggact gacaagagtg caaactgctt tgtcaacacc actctcgatg tcatctggat
      301 aaggctgcct cacttctcag gcgtaggccc gtccgcatct ggaacagctg ctgcaagcag
      361 tacctccacg acctcttcag gtggaggcgg aggcagcagc agtgctcaag tatattcagt
      421 gtctctagga aacaccttgt catcagtagg gcttaagaag aacgatcaag caaagttcgt
      481 ctttggaagc cagacccatt atataactgc gctatctata tcttccacac aggcaaagat
      541 aaggatccaa tcaactcctg tagagatact gatgcaggtt ggccaggaag caaaagtcaa
      601 ccttgatggc gacaactatg acgacctcta tatcaaattt gactcaagct caggaacaaa
      661 ggctacattc agcgtaaaga aaatacgcga ggctatagct tcagtaccat ctgcaccagc
      721 aacaaagccc gcagagaagg cacagccggc aacaacacag caaggacagg ctcagacaac
      781 accacctcaa gttgaggcaa aagaggaagt gccgcctcag gcgattatac ctctgaagaa
      841 aacaacctat ggctggattt atggtgcgct cgcagcggca ttagtgttgc tcatagccct
      901 gtcgctcatc ataagaaagg tcaggaacag ctgatctcaa acaccacaag gtcgtccggg
      961 tttgggcagc ttacttttat ttttcccttc ttttcccagg gaatcttgcc gccgaattgc
     1021 tttgcagtta taaacggcca taagctatgc aaggcataag ggcataagcc agtgtaggga
     1081 tattcatata catgcccttt tctgtgcccg tattcgcact tgcccttgac gctgataacc
     1141 tttatctcga ccataaggat agtgatttta attgctcatt taatatactt tgcgaaaaat
     1201 gggaagggaa ggcttttgtt ggagtgtttg aagctcagtt aatcttatta acccctttgt
     1261 tttaacctcc ttatgctcaa agatttcata atcccattca tagcaatagg catagctgag
     1321 cttggcgaca agacccagtt tgctatcttc tgcctctcag gcaagaccaa aaagtatctc
     1381 agcctctttc tgggtatcct gcttgcattc atcctggcag acggccttgc agtcatcctt
     1441 ggaaacctta tctcaggcat cgtccccggg gtctacatca ggatcttatc aggcatcctc
     1501 ttcatcgcct ttggagtctt tgccctgata aacgccgaaa aagatgagga aaagcttgag
     1561 cttaaaactc cattcctctc agcgttcact ataatatttg tttccgaact tggcgacaag
     1621 acccaggtgg catccggcct ttttgcagca agctattcgc cagtctgggt tttttttgga
     1681 gtcattgctg cattgacggc gttatcgata atagccatct cccttggcag atacatattc
     1741 tcaaggctca acccgaggat catctccact gtctcaggca tacttttcat ctccataggc
     1801 atcttctctt tgtttagata actttttata tcgccctact caaaagaggt ttatgcagtt
     1861 aaaaaaggag atgcccttga tggcattttt gcttctgatg ttcatctctt ctacagtgct
     1921 ctatcgatat gtccctgaga agatacctac ccactggaat gcaaaaggag agatcgatgg
     1981 ttattctggc aagtttttag gcttgttctt gctgcccatc attgcttcta tcctatacat
     2041 cctgtttttg gtccttccag ccataatgcc ttacagaaat aatattgaaa agatagaagg
     2101 gaagttctac tggcttaagg ctatcatgct tgcattcttt gctgtcttat acctagcttc
     2161 aatcctatca agctttggca ttcaaatcaa tattatctat gtgatgatgc cctcactggc
     2221 agtcattttt tactatatag gctatttgat gaagtatacc aaaagaaatt tctttatcgg
     2281 aataaggact ccctggaccc ttgtaagcga caaatcatgg gatatgaccc acaagaaggc
     2341 atctgtattg tttaggcttt atgggcttct gataatgacg accctctttt ttccaagcta
     2401 tgctttcctt ttcctcttga tctctttgat tcctctgatc ctttatcttg tagtatactc
     2461 atacctgata tatagaggtg aaaagaagaa agctgggaga tccttccgat aattttttat
     2521 atctgcccct tctaaaaatc aaccatgcaa gaaagatgca aatgctgtgg cagaatactt
     2581 agggagcatg agaggtattg tgagttctgc ggaaacgact gctattgcaa ggtaaagcaa
     2641 aagcccgctc tttcagtaaa gaccgtccag gcttactgcg tcaagtgcaa ggcaaagata
     2701 agtgtaaaaa acccaaaaga ttacctcatg aaaaacaaaa ggcttgcagt aagaggcata
     2761 tgccctgatt gcggtacaaa ggtctttaag ataaaaggaa tggcaagaaa actaaaattg
     2821 ctcgactgaa accttctcca tatgattgaa ttcttttagt atgcttcgcc ttatggaatc
     2881 aaagtatccg ccgggtacaa gcaactggtc tactggtgca agtaatcctt caccggctgt
     2941 catcctggct attccatcgt atgcagcatt atgcgcccgc cttatctcat ctctgtctgt
     3001 gcagtcaatc ttgccgtcta tgacaagcct gcctatggtg caagcagcag caggaaaaaa
     3061 tattatattg tttgcctgtg caggatggga taatttctta aagtgaggat aatcacaaag
     3121 gcctttctta tctgatatag cattatatat cagctctgga ggcgaacctg ataccaagct
     3181 aaccttgtca aggtcaaaaa atgaaagcac ctgcctcatc gcatcagatg attcaacagc
     3241 aggatttgaa agcaatgcaa aaaatatagg gcttgacctg tcagaaagag gctttaacag
     3301 ttgaggatta tccctgaaat acttccccct cccgcttgtc tcataaaaaa aagctatctt
     3361 atcagcaggg atcttgccaa ggacttctcc taaatatact tctctggcag aatctatccc
     3421 atacgctcga agatctattg cagcctcttg cttgaattta ggaagagatg cagagtcagg
     3481 cctgcccttg tgtatcactc ctctgctgtc agtgcatatc acaggcacgt ccaagccgac
     3541 atgcatcagt gcttttctta ctccctcacc ggcagctcca agcccgttga ttattcctat
     3601 cttgccctga ctgaagtact tccttaaaaa taagccaagc ggagctgtag ccataagccc
     3661 ggtatacagc tggtcgtctg aaaagacata gatatttttc tcacccttca ttattggaag
     3721 cgcagtctcc ataagatcga tgccttttat atcttcaaga tgcacaagga cgcctttgtt
     3781 cctttttgct atagccccta tccctttttt taacgcttta aggatctcac ccagcttgcg
     3841 tgattcgata agaaatattt gcatatgttc agcaaattca tggtcagcaa acctatatcc
     3901 tgcattcaat ttgccggcca ttagcggccc ggcataaagg cctccgacat catatgtaag
     3961 cacagagccg ccgtttgtta ttatgcatcc ccagaaatca agcgcgccac ctatcatatc
     4021 ttctgaaact aaatgagcgt tctcagcagt tacgacgatg ccgtcaaaag attttttagc
     4081 atctccaaat gaaggctgaa gtgttgctcc tgcttctttt aataccctgt ttctaaattc
     4141 aaagccaggc catcttgtta cagcgctgac atgaggggta taagcacctt cttcccctag
     4201 tactttatat ggcgattctc taaacatcct ttcatacgct tccgggtctt ttgctatagc
     4261 gacacagacg agtgtatgct tgagtgcagc ctcaaactgg ttattgcatc tgataaacaa
     4321 ctcttctttg gcaggcgaat caagaagaac ttctttcacc tttacatcta tcatgtcagg
     4381 atggtttgca tcaggcatcc cttcctggac cacttgccaa tatatctctt ttagcctttc
     4441 tctgctaaaa ccgtttctct ctgcaatctc ttgaaaatcc attcaaggta agtctagcct
     4501 catttattta aacctgacgt tgtatgagta acgattagcc tcacttgtac agcttttagg
     4561 tatgtagaaa tataacgacc ccgacaatcc taaactcccc tcttctctat aaccttcgac
     4621 atcccttcga atgtgataaa tgtcactgta tcgccttcaa acactttaga aagaagctct
     4681 tcatcacagt ctgcatcgag agtctcaaag cttatctggt ccatcagctg gactttgttt
     4741 ggcatcttag agatcacctg gccctctttt ctggttatat ctatctggtc tacactgtcc
     4801 tgatggttca ggttcatcgt cttctctcct ttagaataca atccctgaac ataaagcttg
     4861 gtcttggaat ggcagtgcgt cccgcagttt gctatctctt ttcttgtaac ttttagtatt
     4921 tcattgttta tcttaatata atttccaggt tttaggtcct tggcgttggt catagcataa
     4981 agatattgcc tcaattaata aacattgtgt taatcacctt tttgacaact gatatgggca
     5041 tatattttta aagagtgttt taatccgctt tgatagggga attatgggaa gattctttgc
     5101 agaaagcctt gacttggcaa aagttgtaga aagagcgata aatacccagc taaaacactt
     5161 ggtattagga cctccgggag gggggcaagt gctggttata ccttctcttg gtggaaggat
     5221 attggacacg accactgcag gccttgaaga agaaggagtg tcctttgtaa atgaagacgc
     5281 gatgcataaa ggcacaggag gttatctcaa tccaggtaat tttggaggtc ttgatgtttt
     5341 gcgctgggga ccggaggctc tctgctgttt ttatagtggg actccaagga ctgttgatcg
     5401 ctattggact ccccgccaat gggatttttc cagatttttc attgcctccc aattgaagcc
     5461 tcaatcgccc gtgagctctg taaagatgca atccacatta cccatacaat gggttaacta
     5521 ctttagggaa aaaattagta tgagcgtaac caaaagaata gatctatgcc ttaatcctct
     5581 tagagacgat gatagattaa attccttttt caacgaacct gggcattatg taggttatag
     5641 ggtaacagtc gaagccacta acgttggaaa taggccattg agtattgatg gtaggaatgc
     5701 ggtcgcactt ttggcggttg cacaattcga tgccgcgcca caatctaact ggatagtgcc
     5761 cataaccaga gcaagggatg cccccctcct atgggattgc agccagagta ttcattcaag
     5821 acagccacag gctcaattga cggacgatta tctgtcggtc ccgacaaaaa gtgatacccg
     5881 agcgatatgg ggtgtccctc ttgggagata ctgccaacgt gtggcctact acgatcctag
     5941 caggggggag ctagtaatct ttatctataa cataagggat cgccaggcag atttccttaa
     6001 gcaggcttgg aattgcactc ccaccgaaag agggactcta gctgagtcat tgctctgccc
     6061 ccataagcct gtagtcggat tgaattacgg acatcgctct gagtcaggga atgctgcctg
     6121 gccatgtggc ataagcttat acggccctta tatgggtata cctgtaaatg gcagtgtagt
     6181 cttaacttat cactgcctta gatataaagg gcccgaagaa acacttcgca agatcgctgc
     6241 cgacaaggct ttgctaggtg taactataaa gtgattgtta ctatttgatg atagtcacaa
     6301 agagaacatt tataaatgca taataaaacc tttttctaag cactatgagt aatcctgatg
     6361 acaatctagg tcgtatagtt ggagatatag ctaatatact atttggcttg gaaaataaca
     6421 atgcagacca tgcaaaacgc tggctttatg atgcttggca gatgatcatg accggtgcag
     6481 tcacgcctgt gacccaggcc tatattgaca ggtgctctga aggcggcttt gccatgcacc
     6541 atgataataa gagtcttttc gtcaaaaagc tacctaatca acaagctgca gaacagcagt
     6601 atgctttcgc cagaaggtat tcaagcgcag gcaactatct tatgatgaag gcagaaactc
     6661 cttttatata tcaggataaa gggctggtcc tgtatctagt ccattttgcc agaggtatgg
     6721 atcttggggc tttcttttta gaagcgtcaa aaaaaagatc ggcggataat tttgtaaaac
     6781 aggttactga aaaatgtatt gatgatcttg ccttttggca aggtgatgct gggaatgcca
     6841 tggcagcaag accagccgct cctgaggata tattgggcac catgaaggaa aataccctaa
     6901 ggtctgtccg caatgcaggt atatatggag gtatcagtat cagtgaactt gacctggttc
     6961 ttcttgagcg ctcgttgaaa atatttgacg gtttgacttt caaccaacac actatagtgg
     7021 ttggctctga ctattgccca aagaatatgg ggataaagac agattctgat aaagacgact
     7081 tagcttctgt tatgaatgtg atgagttcta atggtgttga tcctgatcca ttcgagatag
     7141 caactttgat ctatcattgg gatccatcgg ccagatggac ccattggctt gaagaccttg
     7201 ctgcttttac gacctcttat gagtttcata acgctaacag aaagactagg ctgtcggact
     7261 ggttgcataa gctctgttca gcacgatctt ccttggatcc ttcattcaaa ggctggatag
     7321 ccgagcaaga gccgtcaagg gttcaccagg aacttagtct gatgatctta tacagggctc
     7381 ttagaaaggc agaccttacg gcgactcttt atgcatggag aaatgaaaat attggccaaa
     7441 aaatatgccc tgcagattat acctcggtaa gtagatcatt ggattcacag ttatcaggtt
     7501 actctgcgac cgtaggtaaa gcagtaagat ggctttctga aggctatggt cctggaagga
     7561 tatatacacc ctcaacagac aaggaagcgg caagtatatt ttcccaatat ggagcagaaa
     7621 tacctgtacc tgtctgtcta ggcttcttca attatcttgc acaaaaattg aggccttgga
     7681 cgatctcata tccggcacta aagcaagtca agaacccttc ggagtttgca agatgacagc
     7741 aatgcttgaa aagagcaatg accatgtcca tacgtggtac tcagatggag agctgactga
     7801 gcttgagctc attcttcttg gacttaccca aggcagccag cgaattactt ttaccgacca
     7861 tgatagcaca cttgcttggg ctcctgagac tattgctaag ctaaaccggt tttttcatga
     7921 aacccttaag gtaagtaaag acggtaagac tagtgctcca tgccctctct ttaggccata
     7981 ctacctccag tatatcatgg atgccgcgag gaaaggagat ttttattttc acgactatca
     8041 caagattgta gagattgagc caggtttctt aaaatgtggc ggccttaccc tcgggaatgg
     8101 tgcagagttg acgacaaaac tagggcagac attccatata cttgggcttg gaatgcaaca
     8161 agtcccttca tatttgtccg agcgtcttga taagatagcc aagcaaagga gaaaccgagc
     8221 caaagaaatc cttgaattct tatacaggga tgattatttt aataaaagaa cccttcaata
     8281 tcttaaggca agtcagggta tcatacctgc gggcgttgac gaggcactta agcggcagcc
     8341 agtgatcata gatgatgttc ttgctgtgaa ccccaatgct ccaagcaggc ttacaattgg
     8401 gtttctttta tctcaaaggt atggtcaaga cgtgtatggc aatctcaccc ctgaacaggt
     8461 tagagacatc tatctccaaa gggaaatgat caaaagccag tttaagattc cttcattctc
     8521 cccaaaagag cagatcaagg ctattcttga cagcggagga tcttccggtt ctgtgattcc
     8581 tgttcttgcc catcctgatg aaggcaggcg ccaaggctgg ctggaaggca attttggcgc
     8641 tcttgaggct ttaggataca gtgttgaaaa aatgctggaa gaaggagcga tagatgaatc
     8701 agatgtcagg tatgctgcaa agaggtcact catggcattg ttcatagaat ggggtatcag
     8761 aggagtagag agcgcagaat atgcaagaca gtatggaata atggatttta gccatgatac
     8821 agattatcat gggccaagaa tgaaagcgac aaggcctatc actaaaggac aagggccaag
     8881 cctaaaatct cttttaaaca taggtgttgt gcaggaagca cataaccacc tatattggaa
     8941 aatcagagca gcttccgaat atcacgcttg gcaggaagca aacataatta cgacctcact
     9001 tattgatagg ttcatacaaa gcggaacatc aactcaagat cagagccaga taataacagc
     9061 tatccctaag gaactaacca gaaggccgac gcttgcagaa taccttagca cattcatata
     9121 ttcgcataat gattattata ccggcacagt gctagatata gatacaaaaa ttaaacaaaa
     9181 gcttgagcag gcagcgcaga gaggccgttt tgtaaaagat agctcagtaa acgagcaaat
     9241 catccattat ctagacgaga acgttgatgt ctgttcaagg aagaagctgc tggtcgagta
     9301 tgttcctcag ctgcataata taggcaaggt cctttcattt ggcatagagg agacgccaga
     9361 aggaagatat agaaccacct tcgtacagca tgacaagatt ggcgccgagt tgatagacaa
     9421 gataagggat gtgctcatgc aagacctggg ccttacgaat aaagagatcg attatatccg
     9481 ggatattgct acagaccatg tacgtgtaca tgaatctata tacaatgtga tggggggaat
     9541 agatgtggaa caagagttag atactcttaa atctaatgtc acccttgact gcctccttgt
     9601 tgcaaaagct gctggaacta tgtcctattc aggaccgcag gattgtcata acaagtattt
     9661 tgcggcagca aacaacctaa tcaaggcatg cactcaaagg attagacgtt gataacaaca
     9721 catggtcctg tcaaacaaaa tattttttgt gttatgacaa gcaagtaatg ggctgtcaac
     9781 ctgtctgcat atttgtcgct tttttcaatt atctgaaaaa ggtcgtttat taactctttt
     9841 cgctttttag cgatgtcgac aaacttattc ttgtcaaatt tgtagaaaaa atcaaagaat
     9901 tctctgagtg tcatgtcagt tttttcaagg ctcttgatgg attccttgcc cagacccctt
     9961 cctaacttat tttttttcca taaatcatgt aattggttac ataaatattt atattgatcg
    10021 gcaagatttt caacatcctc cagtatatag taaaccggtc cgattggcga atatggtgtt
    10081 gttccacctt tattgagcag tctcctgcat gttgtcgtga acctgttatt cgcttcttcg
    10141 aggaaagcga tatttggaag catgctgtag ttattattct tgagcgcatc taccagttgc
    10201 tctgacatcg aaaggagcag caagaacgtc ctttttagta taggatcaaa atcttcaagc
    10261 tctccagata catgttttat ctcacaaaaa ttgttccctt ggttcgtaat ttcaaagcct
    10321 accgcctcct ttccaagggc attctgtatg gcctgcatgt cagcttgtgt cttgtatgtt
    10381 atatatattt catcgacgcc ttttttatat agggcatgta tatatctcga aatcattatc
    10441 cctaagcctt ctgtatttat ctcgaccctg cttggagaaa aactatcttc cgtcgttatc
    10501 agcagccttc caccttcttc cctgatatcc acctcatccc ctttctttat cccatgtaaa
    10561 agcgcccatt tcctcggcaa agaaacaagc tgagtgctgt ctgctatctg tattaccttt
    10621 cttttcataa gccttccccc tttaagagtt aaatatttta ataaattcgt gattattata
    10681 taaacttttc ttttttatgt ataaggtaag ctcagattga atctcttagc tcagaaaatg
    10741 tatctaatcc tctttgtctc cacgtggtta tcattgtcat tatagtttct actattcttg
//
LOCUS       DASRUK010000119         6313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1608700, whole genome shotgun sequence.
ACCESSION   DASRUK010000119 DASRUK010000000
VERSION     DASRUK010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 6313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6313
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608700"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..778
                     /locus_tag="VFF28_05290"
     CDS             209..778
                     /locus_tag="VFF28_05290"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HZX45068.1"
                     /translation="MFYSTEIKDHIRVPPDLFNLDVKEAVVKRIKRKYEGFISKDLGI
                     VIDVKDVKEIGEGIIIPGDGASYYEATFELLTFKPEMQEVVMGKIKDIADFGAFINLG
                     PIEGMIHVSQTMDDFVSFSKDKVLLGKESKKNLKVGDKCRARIIAVSFKDVSNPKLGL
                     TMRQPYLGRLDWIDDEVNKAAPEEGKSKK"
     gene            775..960
                     /gene="spt4"
                     /locus_tag="VFF28_05295"
     CDS             775..960
                     /gene="spt4"
                     /locus_tag="VFF28_05295"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor subunit Spt4"
                     /protein_id="HZX45069.1"
                     /translation="MKKKVCKKDKLFVEGSECPICKGNQFSPNWQGRLNILDANKSII
                     AKKIGITVKGEYAIKVR"
     gene            966..1265
                     /locus_tag="VFF28_05300"
     CDS             966..1265
                     /locus_tag="VFF28_05300"
                     /inference="COORDINATES: protein motif:HMM:NF013450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45070.1"
                     /translation="MDLKITNKQENTLLSRTEITAEAAFFGFPTPSKQEIVKKIAGSE
                     KADEKLIVIKKIENRFGNGKAKVSVYVYKTEEDLEKTEPKKKDKAAAPKAEEKKE"
     gene            1267..1431
                     /locus_tag="VFF28_05305"
     CDS             1267..1431
                     /locus_tag="VFF28_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014123228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27ae"
                     /protein_id="HZX45071.1"
                     /translation="MAKKVEKAGRWQLYETAGGLKRKNKSCPKCGQGTFMASHKDRWT
                     CGKCGYMEKK"
     gene            complement(1874..2755)
                     /locus_tag="VFF28_05310"
     CDS             complement(1874..2755)
                     /locus_tag="VFF28_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45072.1"
                     /translation="MQEEEIKELFSKLSEVRKEVTALRQELNQVDEKKEEFFRKKEEA
                     SSKVRELIRKIKEDREQRNTLTSKVKEEKERRELLNQEVKTQISTLKELNEEKNKIAK
                     KLNLTEDPARLKDQIARLEFKIETEGISFEKEKQMMKVIKDLKKKVKEAEKISGVWSK
                     IHESSKEIDQKKREAEDVHRKIQRTAEESQTKHEDMISLSKEVDKLKAEEEEAFKQFI
                     EFKTKFNEINDKLKAKLLEMNQIREKVDHNTKEKKISKERREEEILKIKEQDVEDKIK
                     RGEKLTTEDLLVFQQKK"
     gene            complement(2832..3149)
                     /gene="rpl12p"
                     /locus_tag="VFF28_05315"
     CDS             complement(2832..3149)
                     /gene="rpl12p"
                     /locus_tag="VFF28_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004066279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein P1"
                     /protein_id="HZX45073.1"
                     /translation="MEYIYAAMLIHKAGGKVEEAKVKKILEAAGVKVDEARVKALIAS
                     LEGVNIDEAIEKAAVAPIAVAAAAPQGGEAKAEKKEDTAKKAEDDKKAQEQAAAGLGS
                     LFG"
     gene            complement(3171..4409)
                     /locus_tag="VFF28_05320"
     CDS             complement(3171..4409)
                     /locus_tag="VFF28_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004557515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HZX45074.1"
                     /translation="MAQTHHVAQKKKAVAKSIQQLIIEYPIIAAVNMENLPAPQLQQM
                     RAQLRGKIELAMTKRRIMKHAIDEIKSKKQNIESLEPYLLGMPALIFTRENPFSLFRT
                     LKKSKTAAPAKAGQTAPADIKVLKGPTSFAPGPVIGELAMLGIKSGVEGGKVAIKEDS
                     VVVKAGQKISQKAAEILTRLGIQPMEVGLNVTAVYENGMIYTREVLDIDDNKFKADLE
                     QAGAWAFNLAIESAYTTKLTINTLLGKAFNDSKALGISQNIIDEGIVDELMAKAERSA
                     LSLKTIANIEVSTAPKAAVQESTKKEESIDDAPEIKKEEEVLEAEKELMKEEKELQRK
                     ANKKEIESDVEEEVKKEVAEEEEKLLEQERMDKDKELETNDKKIAELAAKKSKKHAPN
                     EQEQVEKLAQDLKKKGTLRA"
     gene            complement(4411..5064)
                     /locus_tag="VFF28_05325"
     CDS             complement(4411..5064)
                     /locus_tag="VFF28_05325"
                     /inference="COORDINATES: protein motif:HMM:NF003244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HZX45075.1"
                     /translation="MDKNKVLSSLKQLKEKSSKRKFIQSVDLIVNLKDIDLKKPEQSL
                     NYYVSVHYLRGKKTKVCGLVGPELITQAKATFDNALSVDDFNKYEGNKKDAKKLASAY
                     DYFVAQATIMPRLATIFGRVFGPKGRMPNPKAGCVVPPNANLKPLYERLQKTVKLQTK
                     NDPIVQTMVGMENQSDEEIADNVMTIYDSLVHTLPGGKNNIKSVLLKLTMGKPLRVE"
     gene            5500..5865
                     /locus_tag="VFF28_05330"
     CDS             5500..5865
                     /locus_tag="VFF28_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45076.1"
                     /translation="MPNKKIIYGVIIIAILAASIYGFSYYRNMPGKFNDFAKCLTANN
                     ATMYGTEWCKFCKAQKQMFGKSFEYIDFVDCDRYRQECLSAGANAYPAWKINDEPYSG
                     LQQLERLSALTGCKIEAQT"
     gene            5900..6190
                     /locus_tag="VFF28_05335"
     CDS             5900..6190
                     /locus_tag="VFF28_05335"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZX45077.1"
                     /translation="MLKITSYLGVLVLIISIGGFFEPKLGYFLLMVFATLMIISPFRG
                     RWFCGNLCPRGSFNDWLSKISIKKKIPAFFRSMGFRVPVLASLMGFMVFIVN"
BASE COUNT         1664 a         1308 c         1259 g         2082 t
ORIGIN      
        1 aaaaggttga gttgtggcag accctcaagg ggcttgagat tcgcaaagaa ggagtgacct
       61 atggcacaat cacagattat tgggacaggt ttgagaaaaa tgtatagttt agctgtcgca
      121 tttcagagat gttatagaac atgctagctg actgaatact tacataaaat agcaaggttt
      181 aaaaagaaaa tggatttccg gtaataacat gttctatagt acagagataa aagaccatat
      241 aagggtgcct ccggacctat tcaacctgga tgttaaagag gctgtagtca aaagaatcaa
      301 gaggaagtac gaaggattta tttcaaaaga ccttggcatc gtcatcgatg taaaggatgt
      361 taaagagata ggcgagggga taataatccc tggagacgga gctagctact atgaggctac
      421 ttttgagctt cttactttta aacctgagat gcaggaagtg gtcatgggaa agataaaaga
      481 catagctgat tttggagctt ttatcaacct tggaccaatc gagggaatga tccacgtatc
      541 acagaccatg gatgattttg tctctttttc caaagataag gtactgcttg gaaaagagtc
      601 taaaaagaac ctaaaagtcg gcgacaaatg cagggcaaga ataatcgctg tatcgttcaa
      661 agatgtttca aacccaaagc ttgggcttac catgaggcag ccttatctgg gaaggcttga
      721 ttggatagat gatgaggtaa ataaggcagc accagaagaa ggtaaatcta aaaaatgaaa
      781 aagaaagtat gcaagaagga caagctattt gtcgaaggca gtgagtgccc aatatgcaaa
      841 ggcaaccagt tttccccaaa ttggcaggga aggcttaata tccttgacgc taacaagtcg
      901 ataatcgcaa agaagatagg catcactgtc aaaggagagt atgctatcaa agtaaggtga
      961 attctatgga tctaaagata acaaacaagc aggaaaatac actattatct cgaaccgaaa
     1021 taacagcaga ggctgctttt tttgggtttc cgaccccaag caagcaggag atagtaaaga
     1081 agatagctgg cagcgagaaa gctgatgaga agctaatagt catcaagaag atagagaaca
     1141 ggtttggaaa cggaaaggct aaggtttctg tttatgtcta taagaccgaa gaagacctag
     1201 aaaagacaga gccaaagaag aaagacaaag ctgcagctcc taaagcagag gagaagaagg
     1261 agtaaaatgg caaagaaagt ggaaaaggca ggaagatggc agctgtatga gacagcaggc
     1321 ggcctcaaga gaaagaacaa gtcatgccct aagtgcgggc agggaacgtt catggccagc
     1381 cacaaggaca gatggacttg cggcaaatgc ggatatatgg agaagaagta gattttttat
     1441 cgatgtattt ttatatagtt tctttcgtta tttaacaagt ggccagtgag aaagaaaagg
     1501 ttctgtttag aattcctgag aattattttg agattatttg tcattccaaa aggtctattt
     1561 taaaatcgga attcagcttt catggttata agctaaaagt aacaaagaaa ggcatttttc
     1621 tagataactc ctatccttat agaaaaaacc atagcgtaaa tattcccttc agcagcatac
     1681 tccatgtaaa attagaaaag ggcactgtat caggcggatt gttgttatcc tttttgattg
     1741 atataatttt tcattgcgct gtttttatcg attttaaaaa ttggacatcg aattgagaca
     1801 aattgaagtt agctcttata acaaaaaaga catgcaagct ctgtataata ctattaaaga
     1861 aaatatcaac tagttatttc ttctgctgaa acaccagcaa atcctctgtc gtaagcttct
     1921 ctcctctctt tatcttgtct tcgacatcct gctcttttat cttcaggatc tcttcttccc
     1981 ttcgttcctt gcttatcttc ttctcttttg tgttatggtc gaccttctcc cttatctggt
     2041 tcatctcaag aagctttgcc ttcagcttgt cgttaatctc gttaaacttt gtcttgaatt
     2101 cgataaattg cttgaaggct tcttcctctt ctgcctttaa cttatcgact tcttttgaaa
     2161 gagagatcat atcttcatgc tttgtctggc tttcttcagc tgtcctttgt atcttcctgt
     2221 gcacatcctc ggcttctctt tttttctggt ctatctcttt tgagctctcg tggatcttgc
     2281 tccagacgcc gcttatcttt tctgcctctt tgaccttctt ctttaagtcc ttgatgactt
     2341 tcatcatctg cttttccttc tcaaaggata tgccttctgt ctctatcttg aactcaaggc
     2401 gcgctatctg atcctttagc cttgcagggt cctctgtcaa gttgagcttt ttggctatct
     2461 tgttcttctc ttcgttaagc tcttttagcg tagaaatctg agtctttact tcctggttga
     2521 ggagctctct tctttccttc tcttccttta ccttgcttgt aagggtgttt ctttgctccc
     2581 tatcttcctt tatcttcctg ataagctccc ttacctttga gcttgcctct tctttttttc
     2641 tgaagaactc ttccttcttt tcatctacct gattaagttc ctgcctgagg gctgtaacct
     2701 cttttcggac ttcggatagt ttactaaata gctcttttat ttcttcttct tgcatttgtc
     2761 acgttcactc gtaaggtcat tttttgagaa aattgtttat tttctcgctg ctaaatataa
     2821 taaaaaaaaa tttagccaaa taagcttcca aggcctgcag ctgcctgctc ctgagctttc
     2881 ttgtcatcct cagctttctt tgcagtatct tccttctttt cagcctttgc ctctcctcct
     2941 tgaggagctg ctgctgccac tgcaattggg gctactgctg ctttctctat agcttcgtcg
     3001 atatttactc cttcaagtga agctatcaag gcctttaccc ttgcttcgtc cacctttacg
     3061 ccagcagctt cgagaatctt tttaaccttt gcttcctcta ctttgccgcc tgccttgtgg
     3121 ataagcatcg ccgcatatat gtattccatt ttaaatacct cctgtaaatg ttatgctctc
     3181 aacgtcccct tctttttcaa gtcctgggca agcttttcta cctgctcctg ctcgtttggg
     3241 gcatgctttt ttgatttctt tgctgcaagc tcagcaatct tcttatcatt agtctccagc
     3301 tctttgtcct tgtccatcct ttcctgctct aaaagctttt cttcttcctc tgcgacctct
     3361 ttttttactt cctcttcaac gtcgctttct atctctttct tgtttgcttt tctctgaagt
     3421 tctttttctt ccttcatcaa ttctttttct gcttcaagga cttcttcttc cttttttatc
     3481 tctggagcgt catcaatcga ctcttccttt ttagtgcttt cttgtacagc agcttttggt
     3541 gctgttgaca cttcaatgtt tgctattgtc ttaaggctca aagccgacct ttctgctttc
     3601 gccatcagct catcaactat cccttcatca ataatgttct gggatatgcc tagcgctttg
     3661 gaatcgttaa atgccttgcc aagcaaggta ttgatggtca gctttgtagt gtaagcgctt
     3721 tcaatcgcca ggttaaatgc ccatgcgcct gcttgttcaa ggtctgcctt gaacttatta
     3781 tcatcgatgt caagaacttc ccttgtatag atcatgccat tttcatatac ggcagtcaca
     3841 ttcaggccta cttccatagg ctggattcca agccttgtca agatttcagc tgctttctgg
     3901 ctgatctttt ggcctgcctt gactacgaca gagtcttctt tgattgcgac ctttccgcct
     3961 tctacacctg attttatgcc tagcatggct agttcgccga tgactgggcc tggtgcaaag
     4021 cttgtcgggc cctttagaac ctttatgtct gcaggagcag tctgtcctgc ttttgcagga
     4081 gctgctgtct tgctcttctt taaggtccta aacaatgaaa atgggttttc tctagtgaag
     4141 atcaaagctg gcatgcctaa aagataaggc tctaaactct cgatgttttg cttctttgac
     4201 tttatctcat cgatagcatg cttcattatc cttctttttg tcatcgcaag ctctatcttt
     4261 ccccttaact gagccctcat ctgctggagc tgaggagcag gaagattttc catgttaact
     4321 gctgcaatga taggatattc gattataagc tgctggattg attttgctac agccttcttc
     4381 ttttgtgcaa cgtggtgagt ctgtgccatc ttattcaacc ctcaatggct tacccatagt
     4441 aagctttaac agaacgcttt tgatgttgtt ctttccacca ggcaatgtgt ggacaagtga
     4501 atcatagata gtcattacgt tgtctgctat ctcttcgtca ctttggtttt ccataccgac
     4561 catggtctgg actatcggat cgttctttgt ctgaagtttt actgtctttt gaagcctttc
     4621 atacagcggc tttaagtttg cgtttggggg gacaacacat cctgcttttg ggtttggcat
     4681 ccttcccttt ggaccaaaaa cacggccaaa gatcgtagct aatcgcggca ttatcgtcgc
     4741 ctgagcgaca aaatagtcat aagcagaagc tagttttttt gcgtctttct tgttgccttc
     4801 atacttgttg aagtcatcga cgctcaatgc attgtcaaag gttgcctttg cctgggtgat
     4861 aagttctgga cccacaaggc cacagacctt tgtcttcttt cctctcaaat aatgcacgga
     4921 aacataatag ttcaacgact gctctggctt ttttaggtcg atgtctttta ggtttactat
     4981 caggtcaaca gattgaataa actttctctt agaagacttc tcctttaatt gctttaagct
     5041 tgataatact ttatttttat ccattcttgc ccctcctaaa cgagttagta taacgggttt
     5101 gtgagttaga attatttggt tatttataaa ggttggggtt tttgattttt tagataaatc
     5161 cagcctgatg gcgatgactt atataaaaag ggcctgattt atataaacaa attcccggca
     5221 ttcccggtaa aaatagaaaa tataatcgcc aaagctaaga atgatctgtc catctgcgac
     5281 aaaattcctc tttcagagag cagaaacagt attgtcacaa gctgccgacg ggcagtttcg
     5341 taaaaacaaa tatttaataa ctaaaacttc ctgcctatcg atcggtggtt aaagatctca
     5401 gatctaactt gtaaagaatg cggcaaacag ttcagctcag aagaaggttt agcgcagcat
     5461 atcaaggcaa agcattggga agaggctccc aagaaaagat tgcccaataa gaagataatc
     5521 tatggcgtga taatcatcgc tatacttgct gcatctatct atggattttc ctattataga
     5581 aatatgcctg gcaaatttaa tgattttgcc aaatgcctga ctgcaaataa tgccacaatg
     5641 tatggaacag agtggtgcaa attctgcaaa gcgcaaaaac agatgtttgg caagtcgttt
     5701 gaatatatcg attttgtcga ctgtgacagg tacaggcagg aatgcctgag tgctggagca
     5761 aacgcttatc ctgcatggaa gataaacgat gagccatatt ctgggctgca gcagcttgaa
     5821 agactttcgg cattgactgg atgtaagata gaggctcaaa cctaaccttt aaaaatcaag
     5881 ggttagtttt aacctagcta tgctcaaaat aacatcttat cttggagttt tggtattgat
     5941 aatatctatc ggcgggtttt ttgagccaaa gcttgggtac tttcttctaa tggtttttgc
     6001 tactttgatg atcatcagcc cttttagggg aagatggttc tgcggcaacc tatgccctcg
     6061 cgggagtttt aacgactggc tttcaaagat aagtattaag aagaaaatac ctgctttttt
     6121 tagaagcatg ggctttaggg ttcctgtact tgccagcttg atgggattta tggtgtttat
     6181 agtaaattaa attgaatatt tctcattaaa tggaataaga gatagaactt tttgtttgtt
     6241 gtaccaaaga cttctgaaga atttcataat tgccctttcc atttcaggaa tagtaggata
     6301 aaaccaattg cca
//
LOCUS       DASRUK010000120        11248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_424535, whole genome shotgun sequence.
ACCESSION   DASRUK010000120 DASRUK010000000
VERSION     DASRUK010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 11248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11248
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424535"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VFF28_05340"
     CDS             <1..727
                     /locus_tag="VFF28_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45078.1"
                     /translation="IIVYDEVHRLLPKFGGSGEGLIQLERGAREFRKWGVGLVLISQV
                     LNDFVGEIKANIGTEIQVRTLYEGDLNRISMKYGEDMVKSIVKAAVGTGMVENAEYNK
                     GRPYFVSFRPLLHNPHRLPDQELEQYHKYEEIISDIEYQIEQLGEIQFDTFDLKLELK
                     LLEEKTKSGAFKMVDIYLESIRSKLKDVWDKLGKEPKKKELELIPLEEIMQGIEKARK
                     ERKKKTKDEEKKETEVEKDEPVQ"
     gene            711..1682
                     /locus_tag="VFF28_05345"
     CDS             711..1682
                     /locus_tag="VFF28_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45079.1"
                     /translation="MSLFNKNIPHDIEKDDQVDPQVESEPGAAADTAITTKLEYLKAK
                     IESLEEQNKSTSERFISFNEQLGELRSSGFEMGKNMGELEAKSLKAVEIVNQIKPEEI
                     FEVTQKSDFKIQQLSEKITALQLFTDTIMQDMKSMREDMTVFKDAKEILKLEKDVREQ
                     LISINKAKSVIEADTAKVESIFMDVQDQFNQSQRNTSLVNDMRRALENGLKEIENLKI
                     KNVHLAEKVVLEQLSERLAKLETRPSSRALPLFALKSKDVESINSRLDLIEKEISENY
                     HFTGRLLDELKGLGKLVSSKRKITPKQPKERRSARDALQMMKQMAGK"
     gene            1679..5188
                     /locus_tag="VFF28_05350"
     CDS             1679..5188
                     /locus_tag="VFF28_05350"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HZX45080.1"
                     /translation="MRKITLFLAVLMVLPAAAAITLSPNMQDTYIRESTSSTNYQSAT
                     SLYVGLDATSKPMRMIFWPNLTDIPPGATVQDASLAVYAVSDPDDDQVVSAYRISQNM
                     TIGQTTWNLSQTGVNWTAIGGTYSGWITNTTIGANGYYQFNLTSFVQAVVDNNYSNYG
                     ILFIGFEASGADNKAIRSLEYSTVAQRPNLTVEYVPNVAPTINSHNASPQKVDVSFQV
                     NLTLNWSDSDSSLARGYFCSGGSFNSSAGCLNKTYCNTSNSASNPIICSYTALQNDTS
                     ILQYYAFVCDDTDACSNSTGNFSINHAPNVSVIRPNGDEILNQDLYGSYNVNISVNDT
                     DNDSVLLSIYYSTVQGARQNFLATLNSSDNCTASICIYSFNTSGIGGVYYIDARANDT
                     RLASTDSSDDAFNITTVTDASPPSITGEVIEGNLTAGETATANASIVDGNLLNAWFEV
                     NDSSGIFSRSNMTFVSGSTYSAIFQAGSSGQYKFKVFANDSFGNTNYTRDWIDFNVSN
                     PSASVVSESSPENALQGSIILVKASLNATDPIMGTYAFLNCGSGFTFLNGYPQNTSMG
                     NLSQGVSIAEFAVFTPAANGVYVFNVTFTDSYGNSWNSSNFDTEVVSSIGSDNIFLTA
                     TAGASYYLYDNVTIFARLEDEDGNSLAGQDVNISIKYPDGTYWVNETAMSDAGLGYYN
                     YTRQLNSSQTGSYIVLIRASSQNQLRIAYTSFAGLQRGVLIDIDAYPEILAGSAFGAD
                     ILVTNDLGVYIEVDDLKMSLYDSAGNAVTESVSYSSHPSVGHYSYNYTTIASQPQGNW
                     KVKANATVDGNHYTDEQYWKLTGGPFDVRDISVTDSSVPSLAISVVLENTGSAGQDMT
                     VVWNLTRTDTGEVLDSGSDTRLVPAASEVTYTINPTTSYAGEVKVTFLGYYSGTEKAG
                     AYETFSTTEEASSPSAPSGGGGGGGGGARRPSAQNATIVEPLLIFIHAPDVTVSPGGS
                     VIALVEIKNTGTEPILNAFLTIEDIDKRFISSISPEASLLPKKSHTYLVNFTAGIDDP
                     SRQSIFSYAIKSGKFIFSKKAKFTISEKPTLPGLGLLKEEVGQIKEQLVQLTGFLEKH
                     IQEARVITTVTLGLIALLGIVLFALNFGKITNLIEEHRQRKFARIRKMATIQEPENCN
                     IAELAKNRQDILAEIENLKNLLK"
     gene            5185..5598
                     /locus_tag="VFF28_05355"
     CDS             5185..5598
                     /locus_tag="VFF28_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45081.1"
                     /translation="MKAWLLLAVLIIAGCSKQAILEKEIEIKPSCGLEGDINCIGLNA
                     TNRMILLHLENQAADDFLITEIKLYGAGTCGGERLDGEMLPYLLKSHSQGIIQAVCNT
                     LPESGRFDSPVLLRYKNPKDMILRTIKGNITAEIK"
     gene            5881..7146
                     /locus_tag="VFF28_05360"
     CDS             5881..7146
                     /locus_tag="VFF28_05360"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HZX45082.1"
                     /translation="MVKINPFCGLRYNQEKVKDFNLVITPPYDVINEQQKKEYYASSP
                     YNAARLILPDSPQKAASDLKDWQEKGILKQDDKESIYIYLQEFSFKGKQYLRKSFISL
                     IELEEFGKGVLPHERTLEKPFKGRLELLDATRANFGCVFMLYDDRKKATDKLMDEQCK
                     REPDMMFSDNSGISHKLWKISDNGFIENLKNELVKYQCVIADGHHRYRSALKFRDEHP
                     EIAGGKYCMCGFVNSFSEGLLILPIDRFVHDVDIGREELIERISEHFEVLEIEDVKKL
                     VEILEHTKVLIDKAKNLKNHVFGMYSCADNKSYFLKLKNRGIMDSFFPDRSDAYRKLD
                     VNILHKLIFEGIIGISQEAQSQGTYIEYSKEHSRNISACDRFQYLFFINAPLMREIFL
                     TARAGETMPQKSTFFYPKIYSGLVINKIG"
     gene            7157..8242
                     /locus_tag="VFF28_05365"
     CDS             7157..8242
                     /locus_tag="VFF28_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HZX45083.1"
                     /translation="MANKLFIPGPTEVRQEILKELSTPQIGHRTGEFRELFGGLKPGL
                     RKLFYTKNDVLISASSGSGFWEGAVRNCVSGKVLHAVNGFFSKKWASVSESCGKAAGK
                     IEFAWGKPVDPAAVDKALSSGEYDAFCMVHNETSTGVKSDLLAISKVMKKYPQVIWIV
                     DAVSSLAGTKIEVDNLGIDICLASSQKAIALPAGIAVASISAKAYQRAESVKGRGYYF
                     DFLELKKAYDKDETPYTPCIPHLFALRKQLLRMDEEGLDKRFARHKEMAEYAREWAIS
                     NGFAMFSAEGCHSDTVSCISNTKKLDFKAIKKDMAKKGYSIDSGYGKMNEKLEAEGKP
                     NTFRIAHMGDITLDEVKDLCEKLEEYF"
     gene            8448..9404
                     /locus_tag="VFF28_05370"
     CDS             8448..9404
                     /locus_tag="VFF28_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HZX45084.1"
                     /translation="MKVLIPDKVDKKAIETLQKSGFDVDYKVGTKQEEFAGISADADA
                     MIVRSSVKVDKQVIDAAKKLKIVVRAGVGYDNIDVKYAAEKGIIVENTPFGNTNAAAE
                     HTLTLLMMLAKHVIHSHNKLKQGAWDKKSFEGTEVKGKTLGIIGIGKIGQKVAKVANA
                     LEMSVLGFDPYVDANTLKAMGIIKVEFSDLIKKSDYITVHVPLTDKTKNMISTEQFAA
                     MKDGVRILNVARGGVVDEKALEESLKSGKVAAAALDVYAQEPPICSSLLANDRVICTP
                     HLGGSTKEAQINVGLDAANQIIIALKEGKIENCVNGVKSLRK"
     gene            complement(9408..9582)
                     /locus_tag="VFF28_05375"
     tRNA            complement(join(9408..9421,9454..9475,9543..9582))
                     /locus_tag="VFF28_05375"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9544..9546),aa:Arg,seq:cct)
     gene            complement(9618..10244)
                     /locus_tag="VFF28_05380"
     CDS             complement(9618..10244)
                     /locus_tag="VFF28_05380"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HZX45085.1"
                     /translation="MNLVFLGPPGVGKGTVAKDVVKVKGIPQISTGDLLRAAVKKGTS
                     IGKKAKRYMDEGKLVPDDLVIGLLKDRILEGDCGDGFILDGFPRTIPQAEALEQDIEI
                     DKVINFKASDEMIILRTSGRRVCRNCGAIYHIKNIPPKVPGKCDKCGGELYQREDDNE
                     ETVKKRLEVYRQETAPLIEYYRKKGLLIDVETEQPLPQIVADTLKAIG"
     gene            complement(10430..11044)
                     /locus_tag="VFF28_05385"
     CDS             complement(10430..11044)
                     /locus_tag="VFF28_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45086.1"
                     /translation="MTEYPIPETRVKYKDVFNLKNLYVMMHEAFIEDKWFGKDGPVSG
                     NPGGQHSDVETLYLEKFDQKGLNAGGKEMWIYWRLLKKPEGRYSGYIRYKLNFDWHFV
                     YMEKRQIAYQGKQISVNWGEMELSFNASVETDYKKEWEKHWFLKHWQEIYERRIIDQE
                     IERHKKLLYLDLYRITSMVKRYLDLRVFLPTPEPLYPKLFGMEG"
BASE COUNT         3480 a         2305 c         2601 g         2862 t
ORIGIN      
        1 cattattgtc tatgatgagg tccataggct gctgcctaag tttgggggaa gcggtgaagg
       61 ccttatccag cttgagagag gcgcaaggga attcaggaaa tggggggtag gccttgtcct
      121 tatcagccag gtcctcaatg actttgtagg tgagataaag gcaaacattg gaaccgagat
      181 acaggtaagg acgctttatg aaggtgatct taacaggatt agcatgaaat atggagagga
      241 catggtcaaa tctatagtga aggcggcagt agggacaggt atggttgaaa atgccgaata
      301 caacaaaggc aggccatatt ttgtgtcgtt taggcctctt ctgcataatc cgcataggct
      361 gccggaccaa gagctcgagc agtatcataa gtatgaggag atcatcagcg acattgaata
      421 ccagatagag cagcttggcg agattcaatt cgataccttt gaccttaagc ttgaactgaa
      481 gctgctcgag gaaaaaacaa aatcaggcgc tttcaagatg gtagatatct acctggaaag
      541 catcaggtct aagttgaaag atgtatggga caagcttgga aaggagccaa agaaaaaaga
      601 gctggagctt atccccctgg aagagataat gcagggcata gaaaaagcaa ggaaagagcg
      661 aaagaaaaaa actaaggacg aggaaaaaaa ggagacagag gtggaaaaag atgagcctgt
      721 tcaataagaa cataccccat gatatcgaaa aagatgacca ggtcgatccc caggtggaaa
      781 gtgagccagg cgcagcagcc gacacggcaa tcactacaaa gcttgaatat ctcaaggcaa
      841 agatagaatc cttggaggag cagaacaagt cgacaagcga aaggtttatc tcatttaatg
      901 agcagctggg agagcttcgc tcctcaggct ttgagatggg caagaatatg ggtgagctcg
      961 aggcaaaatc gctgaaggca gtggagatag taaatcagat caagcccgaa gagatatttg
     1021 aggtgaccca gaaaagcgat tttaagatac agcaactaag tgaaaagata actgcattgc
     1081 agctctttac agataccatc atgcaggata tgaaatccat gagggaagat atgacggtct
     1141 ttaaggatgc aaaagagatc ttaaagcttg agaaggatgt tagggagcaa ttgataagca
     1201 taaacaaggc taaatctgtg atagaggcag atactgccaa ggtcgaaagc atcttcatgg
     1261 atgtccagga ccagttcaat caatctcaaa ggaacacctc ccttgtcaat gatatgaggc
     1321 gggcccttga aaatgggcta aaggagatag agaaccttaa gataaagaat gttcatcttg
     1381 cagaaaaagt ggtgctggag cagttgagcg agaggttggc aaagcttgag acaaggccat
     1441 cttccagggc attgcctctg tttgcattaa aatccaagga tgtggagtca atcaactcca
     1501 ggcttgatct catagagaaa gagataagtg aaaattacca ttttacagga aggctcttag
     1561 acgagctaaa agggctggga aaactagtat caagcaagag aaagataacg cccaagcagc
     1621 ctaaggaaag aagatctgca agagatgcct tgcagatgat gaagcaaatg gcaggtaaat
     1681 gagaaaaata acattattcc tggcagtatt gatggttttg ccggcagcgg cagcaataac
     1741 cctctcccca aatatgcagg atacttatat cagggaaagc acatcttcta ctaactatca
     1801 atccgccaca tcactgtatg ttgggctcga tgctacaagt aagcccatga ggatgatatt
     1861 ctggccaaac cttacagaca tcccgcctgg agcaacagtg caggacgcaa gcctggcagt
     1921 ttatgcagtc tcagatccag acgatgacca ggtggtttca gcgtacagaa ttagccaaaa
     1981 tatgactatc ggccaaacaa cttggaatct ttctcagaca ggtgtaaatt ggactgcgat
     2041 cggaggcaca tattccggct ggataacaaa cacgacgatc ggcgcaaatg gatattatca
     2101 gtttaacctg acctcatttg tccaggcagt tgttgataac aactactcaa attacggcat
     2161 attgttcata ggttttgaag caagcggcgc cgataacaag gcaataagat cacttgagta
     2221 ttcgacagtt gcgcaaaggc caaatctcac cgtagaatat gtgccaaatg ttgctccgac
     2281 gataaactcg cacaatgcat cgccgcagaa ggtcgatgtt tcctttcagg ttaacctcac
     2341 tctcaattgg tcagactcag actcttcatt agcaagaggc tatttctgct caggcggcag
     2401 ctttaactca agtgcaggct gccttaacaa gacttattgc aatacctcca attcagcctc
     2461 aaacccgatt atttgcagct atactgccct gcaaaatgat acaagcatcc tgcaatatta
     2521 cgcatttgtc tgcgatgata cagacgcatg ctcaaattcg accggcaatt tttccataaa
     2581 ccatgcccca aatgtttcag tcataagacc aaatggcgat gagatcttaa accaggacct
     2641 gtatggaagt tataatgtca atatatcagt caacgatact gacaatgaca gtgttttatt
     2701 gtcaatatac tattcgactg tgcagggtgc aaggcaaaat tttcttgcaa ctctcaactc
     2761 atcggacaat tgcacagcaa gcatctgcat atattctttt aacacctcag gcataggggg
     2821 ggtgtactat atagatgcaa gagcaaatga cacccggctt gcctcaacag attcaagcga
     2881 tgatgcattt aacatcacta ctgttacaga cgcatccccg ccatctatca ccggtgaggt
     2941 tatcgaaggg aaccttacag caggagagac agcaacagcc aatgcttcga ttgttgatgg
     3001 caacctgttg aacgcatggt tcgaggttaa cgactcatcc ggcatattct caagaagcaa
     3061 catgaccttt gtttctggca gcacatattc cgcaatcttt caggcaggca gctctggcca
     3121 gtataagttc aaggtttttg caaacgacag ctttggaaat accaactaca caagggattg
     3181 gatagatttc aatgtttcaa atccttcggc aagtgtagta agcgagagct ctcctgaaaa
     3241 tgcccttcag ggaagtataa tccttgtgaa ggcaagcctt aatgctacag atccgataat
     3301 gggtacctac gccttcttga actgcggctc tgggttcact ttcttaaacg gctatcccca
     3361 aaatacaagc atgggcaacc taagccaggg agtgtctata gcagagtttg ctgtgtttac
     3421 ccctgcagca aatggtgttt atgtgttcaa tgttacattt acagacagtt atgggaacag
     3481 ttggaacagc agtaactttg atacagaagt agtctcaagc atcggctctg acaatatatt
     3541 cctgacagcg actgcaggcg caagctatta tctttatgat aatgtcacca tctttgcaag
     3601 gcttgaagat gaggatggaa acagccttgc cggacaagac gtgaacatct ctatcaagta
     3661 tcctgacggc acttattggg tcaatgagac tgcgatgtct gatgcaggct taggctacta
     3721 taactatacc cggcagctta actcttcgca aaccggcagt tacatcgtgc tgatcagggc
     3781 aagcagccaa aaccagttaa ggatagcata cacttctttt gcagggctgc agagaggagt
     3841 gctcatcgat atcgatgcat atcctgagat tcttgccgga agcgcttttg gagcagacat
     3901 acttgtgaca aacgatttag gtgtctacat agaagtcgat gacttgaaga tgagccttta
     3961 tgattcagca ggcaacgcag tgactgaatc tgtcagctac tcctcccatc catctgttgg
     4021 ccattacagt tataattata cgaccatcgc gtcgcagcct caagggaatt ggaaggtaaa
     4081 ggcaaacgcg accgttgacg gtaaccatta tacggatgag cagtactgga agcttaccgg
     4141 aggcccattt gacgtaaggg atatatctgt cactgatagc tctgtgcctt cgctggcaat
     4201 ctccgtggtg cttgaaaata caggcagcgc aggtcaggat atgactgtcg tatggaacct
     4261 gacaaggaca gacactggcg aggtgcttga ctcaggctca gacacaaggc ttgtgcctgc
     4321 agcctcagaa gtgacatata ctatcaatcc aacaacctct tatgcagggg aagtgaaagt
     4381 aaccttcctt ggctattaca gcggtacaga aaaagctggt gcatatgaaa cttttagcac
     4441 aactgaagag gcttccagcc catcagcacc ttcaggcggc ggaggaggtg gaggcggcgg
     4501 ggcaagaagg ccctcagccc aaaatgcaac tattgtagaa cctttactta tatttatcca
     4561 tgctccagac gtgactgtat ctccaggagg atcagtcatc gccttggtag agataaaaaa
     4621 tacaggaact gagcccatcc ttaatgcttt ccttacaata gaggatattg acaaaagatt
     4681 tatctcaagc ataagcccag aggcatcact gctgcccaag aaaagccata cctacctggt
     4741 aaattttacc gcagggatag atgatcccag ccggcagtca atctttagct atgctataaa
     4801 gtcaggcaag tttatatttt caaaaaaggc aaaatttact atctctgaaa agcctacatt
     4861 gcccggactt gggctcctca aagaagaggt aggacagata aaagagcagc tagtccagct
     4921 aacaggattt cttgaaaaac atatccaaga ggcaagggta attactacag taacccttgg
     4981 gctgattgct ttgcttggga tagtcttgtt tgcactaaac ttcggaaaga taacaaactt
     5041 gatagaagag cataggcaaa gaaagtttgc acggataagg aaaatggcca ctatacaaga
     5101 gcccgaaaat tgtaatattg cggagttagc aaaaaataga caagacatcc ttgcagaaat
     5161 cgagaatcta aaaaatctac taaaatgaag gcctggcttt tacttgcggt tttgatcatt
     5221 gccggatgta gcaaacaggc tatccttgag aaagagatag agataaagcc tagctgcggc
     5281 cttgagggag atatcaattg catagggctt aatgctacaa acaggatgat tttattgcac
     5341 cttgaaaatc aagctgcaga tgacttcctg ataacagaga taaaattata tggagcaggg
     5401 acatgcggtg gagaaaggct tgatggtgaa atgctcccgt acctgctaaa gtcccattca
     5461 cagggcataa tccaggctgt ttgcaacaca ttgcctgaat caggccgctt tgactcaccg
     5521 gtccttctaa ggtataaaaa tccaaaggac atgatcctac gcacaataaa gggaaacata
     5581 actgcagaga taaaataaaa atcccaaccg ccccaagggg cggggtattt ttagtgttgt
     5641 aaataccgtg gcgagccata acaataaccc agtcaccgca agtgagtggg gtatttacaa
     5701 caaataaaca ataatagggc ggacccgaaa tccggtagcc atcacccatt aaacaatctg
     5761 tgtctttagc cagcgaaata tgtctttttt taaaaattta gcggtttttt gtcaaataac
     5821 tggtttctgt gaaaaatagg tcaaagggtt tttaaacgtg ggtaaatttc aagccgaggc
     5881 atggtcaaga taaatccttt ttgcggcctt aggtataacc aggaaaaggt caaggacttt
     5941 aacttagtca taactccgcc ttacgatgtg ataaacgaac agcagaaaaa ggagtattat
     6001 gcaagctctc cttataatgc tgcaaggctg atacttcctg acagccctca aaaagcggca
     6061 agcgacctga aagactggca ggaaaaaggg atattgaaac aggacgataa ggagtctatc
     6121 tatatctatc tgcaggagtt ttcctttaaa ggcaaacagt acctgagaaa aagcttcatc
     6181 tcacttattg aactagaaga gtttggcaag ggggtgcttc ctcatgaaag gacacttgag
     6241 aagccgttca aaggaaggct tgagcttctt gatgcgacaa gggcaaattt tggctgtgtt
     6301 ttcatgctgt atgatgacag gaaaaaggcc actgacaagc ttatggatga acaatgcaaa
     6361 agagagcctg atatgatgtt tagtgataac tctggaataa gccataagct ttggaaaatt
     6421 tccgataatg gatttatcga gaatcttaaa aacgaacttg taaaatacca atgcgtgata
     6481 gcagatgggc atcataggta taggtcagcg cttaaattca gggatgaaca tcctgaaatt
     6541 gccgggggca aatattgcat gtgcggcttt gtaaattcat tctctgaagg gctgctgata
     6601 ctgccaatcg acaggtttgt gcatgatgtg gacatcgggc gcgaggaact tattgagcgg
     6661 atatcagagc actttgaggt tcttgagata gaggatgtga aaaaactggt tgagatcctg
     6721 gagcatacaa aagttcttat cgacaaagca aagaacctaa aaaaccatgt gtttggaatg
     6781 tattcatgcg ctgataataa gtcatatttt ctaaaattaa agaaccgcgg gataatggac
     6841 tctttttttc ctgacagatc agatgcttac cgaaagctgg acgtcaatat attgcacaag
     6901 ctgatctttg aagggataat agggataagc caagaggcgc agtcccaggg gacatacata
     6961 gaatattcaa aagaacacag caggaacatc tctgcttgcg acaggttcca gtatctgttt
     7021 ttcatcaacg ctccgctgat gagggagata tttttgactg caagggcagg agagacaatg
     7081 cctcagaaat caactttctt ttatccaaag atatattcag ggcttgtaat aaacaagata
     7141 ggataggggg ttttcaatgg caaacaaact tttcatccca gggccgacag aagtcaggca
     7201 agagatacta aaagagctta gcacgcccca gatagggcat aggaccggtg agtttaggga
     7261 gctatttggc ggcctgaagc ctggcctgcg caaattgttt tatacaaaga atgacgtgct
     7321 aatatctgcg tcatcaggct ctggattttg ggaaggggct gtgcgcaatt gtgtgagcgg
     7381 aaaagtgctt catgctgtga atggattttt ttcaaagaaa tgggcgtctg tctctgaaag
     7441 ctgcggcaag gcggccggaa agatagagtt tgcatgggga aagcctgtcg acccagcggc
     7501 tgttgataaa gcgctttcca gtggcgagta tgatgctttc tgcatggtcc ataacgaaac
     7561 atccactggt gttaaaagcg atcttttggc gatatccaag gttatgaaaa aatatcccca
     7621 ggtaatatgg atagtggatg ctgtctcatc ccttgcagga acaaagatag aagttgacaa
     7681 tcttgggata gatatctgcc ttgcttcttc acagaaggcg atagcgcttc ctgccggtat
     7741 cgctgtcgca tcaatcagcg caaaagcata tcagagagct gagagcgtca aaggcagagg
     7801 gtattatttt gacttccttg agttgaaaaa ggcttacgat aaagatgaga caccatatac
     7861 tccctgcatc cctcatctgt ttgcactgcg aaaacagctt ttgcgaatgg atgaagaagg
     7921 gcttgataaa agatttgcca ggcataagga gatggcagag tatgccagag aatgggctat
     7981 cagcaacggt tttgcaatgt tttcagcaga aggatgccat tcagacactg tatcatgcat
     8041 atccaataca aagaagcttg attttaaggc tatcaaaaaa gatatggcaa agaaaggata
     8101 cagcatagac tcaggatacg gcaagatgaa cgagaagctt gaggcagaag gaaagccaaa
     8161 cactttcagg atcgcccata tgggtgatat cacacttgat gaggtcaagg atttgtgtga
     8221 gaagttggag gaatattttt agttatagtg atagattgaa aaaaaccatg aagttacgga
     8281 cagataaacc aacaataatg gactcaaaca atgaataatc attaacaagt gaaagccaac
     8341 aataatgact tcgacggttt tgctatgcaa aacctgcaaa ccaccccgac gggggcaacc
     8401 cccggtttgc cttgtcattt gttggctttt gcagaaaagg tgagaacatg aaggtattga
     8461 ttccagacaa ggttgataag aaggctattg aaacactgca aaaatcaggc tttgatgtgg
     8521 attataaggt agggacaaag caagaggagt ttgcaggaat aagcgcagat gctgacgcca
     8581 tgattgtcag gagcagcgta aaggtcgata agcaagtgat agatgctgct aaaaaattaa
     8641 aaatagtggt cagagcaggc gtcggttatg ataatatcga cgttaaatat gctgctgaga
     8701 aaggtatcat tgtcgaaaac accccatttg gaaataccaa cgctgctgct gagcatacac
     8761 tcactttgct tatgatgctt gcaaagcatg tgatccattc tcacaataag cttaagcagg
     8821 gtgcttggga taaaaaatcc tttgaaggaa cagaggtgaa aggaaaaaca ctagggatca
     8881 tcgggatagg caagatcggg cagaaggtag caaaggttgc aaatgctctg gagatgtctg
     8941 tcttgggatt tgacccttat gttgatgcaa ataccctgaa agctatgggg ataataaagg
     9001 ttgagttcag tgatcttatc aaaaaatcag attatatcac agtgcatgtt cctttaaccg
     9061 ataaaacaaa aaatatgatt tcgaccgagc aatttgcggc aatgaaggat ggagttagga
     9121 tattaaatgt tgctcgcggc ggtgtagttg atgaaaaagc cctggaggaa tcattaaaat
     9181 ccggcaaggt ggctgctgct gcgcttgatg tgtatgcgca agagcctcca atatgcagct
     9241 cactacttgc taatgacagg gtgatttgca cgccgcattt aggaggctcg actaaagagg
     9301 cccaaataaa tgtaggactt gatgctgcta atcagataat catagcgctt aaagaaggaa
     9361 agatcgagaa ctgcgtgaat ggggtaaaga gtttaagaaa ataaaagcgc gccccatggg
     9421 actttcaaac cctgagggat ttcctctggg atattcgaac ccacgaccta cagctttcat
     9481 ccgcctcagc cactctcact ctgccaagtt ttaaggcact agcgaaatac tcttcatcga
     9541 cttaggaggc tgtcgctcta atccaagctg agctaagggc gcatcaaaac ttgaagaata
     9601 ataaattata tttaagctta tccaattgcc ttgagggtgt cagcaacaat ctgaggcaat
     9661 ggctgctctg tttcgacatc tatcaataag cctttttttc tatagtactc aatgagagga
     9721 gctgtctctt gcctatagac ctcaagcctt ttttttacag tctcttcatt atcatcttct
     9781 ctttggtaca actctcctcc gcacttatcg cattttccag gcactttggg agggatattc
     9841 ttaatgtggt atatcgcgcc acaattcctg cagactctcc tgccagatgt ccttaggatt
     9901 atcatctcat cacttgcctt gaaattgata accttgtcaa tctctatatc ctgttctaag
     9961 gcttctgcct gaggaatggt cctgggaaaa ccgtctagaa taaaaccgtc gccgcagtcg
    10021 ccttcaagta tcctgtcttt aagaaggcct atcaccaaat catcaggaac aagctttccc
    10081 tcatccatgt atctctttgc tttctttcca atagaggtgc ctttttttac tgctgcccta
    10141 agcaagtctc cggtagatat ctgaggtata ccttttacct ttacaacgtc ttttgctacg
    10201 gtcccttttc caacgcccgg aggccctaaa aataccagat tcattctttc accttgaaaa
    10261 caattaagcc cccggggaga gttgcactcc cgaccttctg attacttaga aacggagttt
    10321 cgaaggatcg gaaaattcgt ttccaataca agtcagacgc aatggctact atgctacgga
    10381 ggcatctttg ctttacagaa aatgtaaaat acttaaaaaa cttctggttt tagccttcca
    10441 taccaaacag ttttggataa agcggctcag gagtaggaag gaataccctt agatcaagat
    10501 acctctttac catgcttgtt atacggtaaa gatccagata gagcagtttt ttatgcctct
    10561 caatctcctg gtctattatc cttctttcat agatctcctg ccaatgcttg aggaaccaat
    10621 gcttttccca ctccttctta taatcagtct caacagaggc attgaatgat agttccatct
    10681 ctccccaatt gacagagatc tgcttgccct ggtatgctat ctgccttttt tccatatata
    10741 caaagtgcca atcaaaatta agcttatacc tgatgtagcc agagtagcgg ccttcaggtt
    10801 tctttaaaag tcgccaatat atccacatct cctttccgcc ggcattaagg cctttttggt
    10861 cgaatttttc caagtataat gtttcaacgt cgctgtgctg cccgccagga ttgcctgata
    10921 cagggccgtc tttgccgaac catttatcct ctataaatgc ctcatgcatc ataacataga
    10981 gattttttag gttaaataca tctttgtact ttacccttgt ctcaggaatg ggatattctg
    11041 tcattaatcc agcctctggc aaatttttat ttaaactttt cctttttgag gcattgttaa
    11101 atcttcggca gattaatata gtacctcgtt ctattgacat ttgaggtgat attatggagg
    11161 tattttgcat tttctgcaaa aagtcaacaa accttgtcaa agcaggtttt cgcatcaaca
    11221 agacaggcaa gaaacagcgc tacctttg
//
LOCUS       DASRUK010000121        14430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_752203, whole genome shotgun sequence.
ACCESSION   DASRUK010000121 DASRUK010000000
VERSION     DASRUK010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 14430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14430
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752203"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..964
                     /locus_tag="VFF28_05390"
     CDS             227..964
                     /locus_tag="VFF28_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45087.1"
                     /translation="MVDFEDEQFTRLILNNLYRLGTDKINIQVNNIRELEAIHRLTTN
                     NFLGFEITKESKDHCSIESVTEPHDEKYEILLRRIFLIIKDCLGMIEEDFRKRDFALN
                     SIIERQFFKVEQFANFCMRTAIKNSGNKSSMQYLFVYDLLVMQGELKHMYEFIGQKGS
                     TVNPKTMQLVKKIKSLFDKLYHIYFGEGFSQLPELNKELKGLLNADIYSLLPLLRGKE
                     VVIVYHLAVFLRLLTLIISPAIALKSE"
     gene            complement(950..3043)
                     /locus_tag="VFF28_05395"
     CDS             complement(950..3043)
                     /locus_tag="VFF28_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45088.1"
                     /translation="MEKYADLLSQYTDYSERVQLKNLLDKEQGKGTSSKRLVAGSLFF
                     LRDHLFYKDYCELEEMLLAEKDDQVQMAAAESRGCMVHRDDYMPIFKERKNGTRAVQM
                     GNARSFAYTAWHMHSKQEDIGYFVQNERLYQAASRSELKVQYTLDLAAGAPFNPAVCV
                     IYIKQRLHSKNPEVRRAAVYASLGLVLSNQTLDQLTPEVNNVLAEMDLNGYKIPGKNL
                     WAASIDDRRKILCTEKRMPVSDFDSFKAYVDMYRQFYERRDHHTKTIPKFADAKTQNY
                     AEGMVISARQAIANRGSYRAAEAFLRSPLPKQGGTSKGLGLLFSKEIDYKRVRDGIEL
                     ASHYHRLAAQERDGPWSSLYARLLKRRANLATLANVVACSEMNPDNAALLLSLARCCT
                     KEVYIGVNKWMYANFPMESLIPQDLRVYKYYRPTEKGSQILRANDRRIEDAVTVSMKA
                     GIQKIREYPNISGVIEAYYDPRVQSFVIIEEGAHGSLQDIIGEPVDVPVVLDLTFQML
                     KSQDQARQMGISLKDIKPSNFLLRWLDSKLVVMATDLDQAEILGILNMAKRTKATEHP
                     LYCDFRKLCTDDTCNPITDRFPIGLIEYELAMGEHAYVELLDGLSIPKDGSAEWNRIS
                     YDEREKTVDQPIKETLIRMGHRFDDYIGDSIRTKFPGKEGDALIATCLPMVTFDLKKR
                     ENPFKKGHPPGLFTL"
     gene            complement(3044..4495)
                     /locus_tag="VFF28_05400"
     CDS             complement(3044..4495)
                     /locus_tag="VFF28_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017744157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZX45089.1"
                     /translation="MKPSILIVEDEAIVAKDIENIIKRKGYESAGIVSTGKEALYEAR
                     AKNPELVLMDIRIKGGMDGIETAGKIKSELGIPVIYLTAYADDKTLERAKVTEPFGYI
                     IKPFQEREIHTSIEIALYKNKAEKDKRALSDKIIKLTRRIPLTDNEKQVMYSMVRHPL
                     LNDIDLSKKLKIKRSTITAIRNRLKSDNLYTEYRIPNFAALGCELVVPVYADLNPLVG
                     SNKAKILFDAGHAPEQVFLLATNRTFAGIAISQNFTEFKKHLDKTSSQFKDSNLSSDI
                     EASYFPYQISSVEKFFDYSQFLHGLFDLSLENDKEQDIKVEQRKLTKSEKKVLYALTL
                     YKELNDSEIAAKIDVPRTTLSQIRRRLVSDGLIRFVNIPNLLELKCELMIVSHIKLHP
                     SEKNIDKVCDYIKSKPSCTLLIRKELEAFSLNVYNDYNDYEIEVTDDSKFFNKNQILI
                     EEQANKIMTLPQVQYRKLDFAPLLKKVLEMGKD"
     gene            complement(4492..6024)
                     /locus_tag="VFF28_05405"
     CDS             complement(4492..6024)
                     /locus_tag="VFF28_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HZX45090.1"
                     /translation="MEGAVLSKTSQSIARHAHGVFEHELRNLRSKFLVCQKAKLSDTH
                     KSPADPGLKCQERASKAISDMSRSGLKPDLGLDAVMASMRCPVVVFDKTNKIIRINKE
                     ARSVISLGKANMSSISKISEDALAGKTVRDESILFKDRKGLVRTAFVSASPIYGRGDI
                     IGDVVIWHDITERKKIEESLRASERRHSLLKASTPMVIIELNPFFQITDWNPGARNTF
                     GYEEKEVLGRNLWEIISTKQENHIWSKITHRRMSVHAVRENKSKQGKKIICEWFITPL
                     IDESSNTVGITCIAKDITSQKEAEVKLKRSLDEKEMLLKEIHHRVKNNMQVMSSLLSL
                     QSRKFDDERLSKAMAESMGRIKSMALIHEMLYKSGEYSNIDFSKYLMDLTNDLGRTCG
                     SPRVSIKLDCEEAHIPMDQAISAGLLVNELITNSLKHAFPDNREGKVKVSLRARGKTY
                     KLTVEDDGIGLPPNLDPSNSRTLGLELAVTLARQLGGKLKLYRKKGTRFEVEFAKERD
                     KR"
     gene            complement(6324..7394)
                     /locus_tag="VFF28_05410"
     CDS             complement(6324..7394)
                     /locus_tag="VFF28_05410"
                     /inference="COORDINATES: protein motif:HMM:NF024790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZX45091.1"
                     /translation="MRSSLRIGRIFGIGIYLHATWFLIFFLLTYSLAQFYFPEYYDFS
                     AALDWVLAFFSALLLFLSVLMHELSHSIVSKKEGMEVESITLFFFGGVAAASAEETSP
                     KSEFLLAAAGPLFSAVLGLVFLALYALDIEMHLTAIFDYLYKINFILAVFNLIPAYPL
                     DGGRILRSILWAVQKDIRKATLIASKGGVLFGYMLMFLGIAGMFAGGGSVWYILIGAF
                     LVFLANESYRQLIIKENLKKISLDGHVMKKTLIDKDLPLSKFFDWCVANHLSFGIVKE
                     REYFIADIDKLNDAQNIGSMKVKDIMQAVSPIDSEDDAYHILVELKKKNVKGAPVTRK
                     GRYIGIVTVESLMNMARIKSMR"
     gene            complement(7391..7723)
                     /locus_tag="VFF28_05415"
     CDS             complement(7391..7723)
                     /locus_tag="VFF28_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45092.1"
                     /translation="MEAAAFVVQKHEISMLQYDFRVVFKDMIKSWAIFRGPSEQKQLA
                     IEVDHADSLDCKLWDEGTCLIQQDLEAALKSGKLSLILEGEKLRGYFVLQKLEKGNVW
                     LFKKERIQ"
     gene            complement(7733..8152)
                     /locus_tag="VFF28_05420"
     CDS             complement(7733..8152)
                     /locus_tag="VFF28_05420"
                     /inference="COORDINATES: protein motif:HMM:NF021536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HZX45093.1"
                     /translation="MIRSIERSLDKTYEWFNDIQKELGWENPQMAFEASKAVLQTLRD
                     RLPVEEAAEFAAGLPMLMKGVYYEGYKPSRKPKRIKKAEDFYAEVQKRTTQPIQPEDA
                     TKAVLTVIESKISRGEIEDVLSNIPTKLRQIFIPKIK"
     gene            complement(8157..8897)
                     /locus_tag="VFF28_05425"
     CDS             complement(8157..8897)
                     /locus_tag="VFF28_05425"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HZX45094.1"
                     /translation="MAQDIKLEDGEKIHLERPIIGPTIICGFQGFGMVGALVVNHLID
                     EMDFKEIGSSQLREVPPLIVFKGKRILKPVSIFFNSINNIVLISILAPTVGIEWNIAD
                     MIETIAKSMKAKEIIIPDGIIAEDHQSIFYISNFGKDLQSIAKPVENSVVAGVTSALL
                     MKRNLPVICLLGNMKSDKFGAMAAAAPVLTSGITATNLVKVINRYLNLDVSTERLEQA
                     SKDMEKELGSFIDQLSRAKSDREQRYIG"
     gene            complement(8907..9242)
                     /locus_tag="VFF28_05430"
     CDS             complement(8907..9242)
                     /locus_tag="VFF28_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45095.1"
                     /translation="MRWNGIIFLIGLAIAVIIAIFGAATIPTWAIWVLGILGIIAGLF
                     NIAAEEVTPFLMAAIAFLISFQALAQVLTTVLLGYEAIGTFFGLLNVFVAPATAIVAI
                     VALFRLLRD"
     gene            9404..10021
                     /locus_tag="VFF28_05435"
     CDS             9404..10021
                     /locus_tag="VFF28_05435"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010884554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex kinase/ATPase Bud32"
                     /protein_id="HZX45096.1"
                     /translation="MKNKVQVFMKKIAQGAESKIYRKDDKIVKVRFEKKYRIKEIDSR
                     LRQARTRREKKILDKLAKIGFKSPALIESDGKEKIEMQYIDGPKVRDILEKSQELSEE
                     IGRKVALIHNHGIIHGDLTTSNMIYNKEVYFIDFGLSFFSHKLEDKAVDLHLLKQALE
                     SKHYKIWESCFDAALKAYVKEAKDGKEVLKRLEIVEKRGRYKGKR"
     gene            complement(10022..10912)
                     /locus_tag="VFF28_05440"
     CDS             complement(10022..10912)
                     /locus_tag="VFF28_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45097.1"
                     /translation="MTDGLEQRIVLFKEPLSESQTTASCVKITDFDSILARHHQQYGS
                     SISHWKEIALVLLTPYMEALSGKYPVDPRRLYLDSTTSYEVLAAREGKAKHITPIGTN
                     GLIRTADGYLLYGLRGGQVEAGQACIVPSGSISAKPEEASERFYTNPIFERFESEAAT
                     EAGLSSHELKNARLIGYVTDPGHTKSIQFVIAVDTHLTFDEIKQRHEAAYSVYAQKKR
                     ELTDTISENEADLQAREAISGAGFINTSAWEHTGLIGIKGDQLATIISSNQVSYNGKH
                     YQLTNIGAGCLRLYQKLISR"
     gene            11022..11690
                     /locus_tag="VFF28_05445"
     CDS             11022..11690
                     /locus_tag="VFF28_05445"
                     /inference="COORDINATES: protein motif:HMM:NF016831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZX45098.1"
                     /translation="MWVAKLKLKHDDCVIGRRCKRFHVISIGIPFNSYKEADKQYFSH
                     FETLVGEENSIQRFIEDVKDDPAIHSLEVQGSSIFFINEVPIQHTIPTTHYNPKIFFI
                     KPVVVDETGSESWEIGSWDETVLRNFIVNLQKEHFEVKILKLQNEKINEIYFPQVMPF
                     LTKGQKRALEIATQRGYYDYPRNVELKDLAKEAGISLSTFREHLRKAEKKIMPDLIRN
                     VRDE"
     gene            complement(11691..12212)
                     /locus_tag="VFF28_05450"
     CDS             complement(11691..12212)
                     /locus_tag="VFF28_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012459409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HZX45099.1"
                     /translation="MRFVIFFVLALVLGCQGQQNVDKIENNTEVGKMKLMSDDFKQGD
                     TIPKKFTCQGEDINPQLSWEDVPAGTMSFALVIDDPDAASGNFIHWMVKDIPADVREI
                     PQDSIPGVQLKNSFGRENYGGPCPPALHRYFFKLFALDVEKLTAEDKTELYQQVNLHT
                     IAQAELMGKYQKI"
     gene            12284..12799
                     /locus_tag="VFF28_05455"
     CDS             12284..12799
                     /locus_tag="VFF28_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45100.1"
                     /translation="MSINYESLLYDRYEITAILFVRIADHWSVVQKANSAETHGCCPL
                     EWRINESLKPKNPKKTMIGKRMPPESFFLDADIHLQRDKSLADMHCCLEVDKDGLWAH
                     DLSTYGIAGKDYQGNTYSNINAPQKPIRQLLVPGNIEEKSARCFISFGHYALRFDLKM
                     TEPSNHQKIYK"
     gene            12818..13837
                     /locus_tag="VFF28_05460"
     CDS             12818..13837
                     /locus_tag="VFF28_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013468302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--phosphoribosylaminoimidazolecarboxamide
                     ligase"
                     /protein_id="HZX45101.1"
                     /translation="MVADNYTIATLGSHSALQILKGAKDEGFRTVCICIKGREKLYKQ
                     FKVADKIIIINNYGELENIQDELLKENPILIPHASFLTYMGFEKVEQLKIPYFGNKKI
                     LRWESDRNMERKWLTEAGLKTPMIFQKPEDIDRPVIVKFHGADGGKGYFLAKNTRDFR
                     KKIKLHPGKGYVIQEYILGVHMYAHYFHSPLTEETEIMGFDKRYESNVDSLGRISARD
                     QMALPKEKVDPSYVIVGNIPIVVRESFLQRFIEKGENVVKVSKKIAPPGLFGPFCLET
                     IMTPEEDIITFEISARIVAGTNPYIHGSPYSWLKYDEPMSTGRRIAREIKLAIEQGQL
                     KKVLG"
     gene            13834..>14430
                     /locus_tag="VFF28_05465"
     CDS             13834..>14430
                     /locus_tag="VFF28_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1246 domain-containing protein"
                     /protein_id="HZX45102.1"
                     /translation="MKKGVFHHLFKGVQIEVSRVERNEAKDSGKKQAENPFAHLERKP
                     LKISSGTKISEILDHYDKRAITIGALGGHSALDVCRGAKKYGFRTVVVCQKGREATYA
                     KYYKARDSGKGIVDEVILVDKFSDITKKDVQQKLRDLNTIFVHNRYFWVYCNFKDIEK
                     NFKVPIYGTRSMVKLEERDQPKNQYYLLSKAGVRMPKLF"
BASE COUNT         4119 a         3191 c         2880 g         4240 t
ORIGIN      
        1 ccttgttttc tttgttatac agtctcttaa aaatacttga cgaatgatga aatttggttc
       61 ttaatgccat ctatatcacc tcttgctaat tacagaaggg gtacttatat aagaactttt
      121 ctatttaact tactataagc agggtgatga acttgagata accgaaaatg gaagggttat
      181 aacgcttata tctgactcca aggctgaccc aaaagagaaa gcgatcgtgg ttgattttga
      241 ggacgaacaa tttacccggc ttatccttaa taatctctac cgcctaggta ctgacaagat
      301 aaacatccag gtaaacaaca tccgtgagct tgaagcgatt catcgtctga ctactaataa
      361 tttcctgggc tttgaaataa ccaaggaatc caaggatcac tgcagtatag aaagcgtgac
      421 tgaaccgcac gatgaaaaat atgaaatttt gctccgaagg attttcctga taataaagga
      481 ttgcctaggt atgattgaag aagatttccg caagcgggac tttgctttaa actctatcat
      541 cgagcgtcaa tttttcaagg tggagcaatt tgccaatttt tgtatgagga cagctatcaa
      601 gaattcaggc aacaagtcaa gtatgcagta tctctttgtc tatgacctcc tagtgatgca
      661 gggagaactt aagcatatgt atgagtttat aggacaaaaa gggagtacag taaatcccaa
      721 gaccatgcag ttggttaaaa agataaagtc attgtttgac aaattgtacc atatatactt
      781 tggagaggga ttctcccagc ttcctgaact taataaagag cttaagggac tgttgaatgc
      841 agatatttat tcgttgctgc cattactaag aggaaaagag gttgtgatag tttatcatct
      901 agccgtgttc ctgcggctct tgacattgat aataagccct gccatagcat taaagagtga
      961 ataaacctgg gggatgccct tttttgaagg gattttctct ttttttaagg tcaaaagtca
     1021 ccatgggtaa acaagttgca atcaatgcat ctccttcttt tccagggaat tttgttctga
     1081 ttgaatcgcc aatataatca tcaaacctat gtcccatgcg aatcaaagtt tccttgattg
     1141 gttgatctac agttttctct cgttcatcat aactgatgcg attccattcc gcactcccgt
     1201 ctttagggat acttaaaccg tccaagagtt caacgtaagc atgctctccc atggcaagtt
     1261 catattcgat aagccctata ggaaacctat ctgttatagg attacatgtg tcgtcagtgc
     1321 agagctttcg gaaatcgcag tacaaggggt gctctgtagc cttggttctt ttggccatat
     1381 taaggatacc taatatctca gcctgatcca agtctgttgc catgactacc agcttagaat
     1441 ccagccacct caataggaag ttggaaggct tgatgtcttt taagcttatc cccatctgtc
     1501 ttgcctgatc ctgagatttc agcatctgaa acgttagatc cagcacaact ggtacatcaa
     1561 caggctcgcc aatgatatcc tgaagagagc catgggctcc ttcctcgatt attacaaatg
     1621 attgtactct tggatcataa taagcctcta taacccctga tatgttcgga tactccctga
     1681 ttttttgtat ccctgctttc attgacacag tgacagcatc ctctatacgt ctgtcgtttg
     1741 cacgtaatat ctggctgcct ttttcagtag gcctgtagta tttgtacact ctaagatcct
     1801 gtggaatcaa tgattccatc ggaaaattcg catacatcca cttatttact ccaatataca
     1861 cttcctttgt acagcacctc gcaagggaaa gcaacaaggc tgcattatca ggattcattt
     1921 ctgagcaagc gacgacattt gctaacgttg caagattagc ccttcttttg agcagccttg
     1981 cgtataggct tgaccatggc ccatctcttt cttgggcagc aagcctatga taatgtgaag
     2041 caagctctat tccatccctc acgcgcttat aatctatttc ttttgaaaaa agaagaccca
     2101 accctttact ggtcccccct tgctttggaa gagggctcct gaggaaggcc tcagctgctc
     2161 gataactgcc cctattagca atggcctgcc tggctgatat aaccattccc tcagcataat
     2221 tctgggtttt tgcatctgca aatttgggga tagtttttgt atgatgatcc ctcctctcat
     2281 agaattgcct atacatatct acataagcct taaacgaatc aaaatcagaa acaggcattc
     2341 tcttctcggt gcaaagaatt tttcttctgt catcgatact ggcagcccat agattttttc
     2401 ctgggatctt atacccattc aagtccattt cagctagcac gttattaact tcaggcgtca
     2461 gctgatcaag tgtctggttt gaaaggacta gtcccaaaga ggcgtatact gctgcccttc
     2521 tcacctctgg atttttggaa tggagccttt gtttaatata tatgacacat acggccggat
     2581 tgaagggagc gcctgcagca agatccaggg tatattgtac tttcaactct gatctgctgg
     2641 ccgcttggta cagcctttca ttttgtacga aatatcctat atcttcttgt ttagaatgca
     2701 tatgccaagc agtatatgca aacgagcgcg cattgcccat ctgaacagcc ctagtgccgt
     2761 tttttctttc cttaaaaata ggcatataat catctcgatg gaccatgcag ccccttgatt
     2821 ctgcagctgc catctgtacc tgatcgtcct tttcagcaag aagcatttct tctaactcac
     2881 aataatcctt atagaaaaga tgatctctaa gaaaaaatag ggatcctgca accaaacgtt
     2941 tgctgcttgt tcctttgccc tgctctttat ctaatagatt tttcagctgg acccgctctg
     3001 aataatcagt gtactgcgaa agtaaatcgg catacttttc cattcaatcc tttcccattt
     3061 caagcacctt cttaagcaaa ggcgcaaagt caagcttcct gtattgcacc tgcggaagtg
     3121 tcattatctt gtttgcctgc tcctcgatga gaatctggtt cttattaaaa aacttgctgt
     3181 catcagttac ctctatctca tagtcgttat agtcattgta tacattcagg ctaaaagctt
     3241 caagctcttt tcttatcaaa agagtgcatg aaggcttgct cttgatataa tcgcacacct
     3301 tgtcgatgtt cttttcgctt ggatgcagct ttatatggct cactatcata agctcgcatt
     3361 taagctcaag caggttaggt atgttcacaa acctgataag cccgtcactt acaagccttc
     3421 tccttatctg tgaaagggtc gtccttggca catctatctt tgccgctatc tctgaatcgt
     3481 tgagttcctt gtacaaagtc agggcataaa gaactttttt ctcgctcttg gtaagcttcc
     3541 tctgctcgac cttgatatcc tgctccttgt cgttttccaa agacaggtca aacaatccgt
     3601 gcaggaactg gctatagtca aaaaattttt cgactgagct tatctggtaa ggaaaatacg
     3661 atgcttcgat gtctgatgag agattgctgt ccttaaactg gctgcttgtc ttgtccaggt
     3721 gctttttaaa ctcagtaaaa ttttgagata tggcgatgcc agcaaatgtc ctgtttgtcg
     3781 caagcaaaaa gacctgctca ggggcatggc ctgcatcgaa aaggatcttt gccttgttgc
     3841 tgccgacaag cgggttaagg tctgcataga cagggaccac aagctcgcat ccaagtgcag
     3901 caaagtttgg tatcctatac tctgtataaa ggttgtctga ttttagcctg ttcctgatag
     3961 cggtgatggt agatctcttt atttttagct tctttgaaag atctatatcg ttaaggagag
     4021 gatgcctgac catcgaatac atcacttgct tctcattgtc tgtaaggggt atccttcgtg
     4081 taagcttgat tatcttatcg gaaagcgctc tcttgtcttt ttcagccttg ttcttgtaaa
     4141 gcgctatctc tatcgatgta tgtatctccc tctcctggaa gggctttatt atatatccaa
     4201 aaggctctgt gactttcgcc ctttcaagag tcttatcgtc agcgtatgca gtaaggtaga
     4261 tgaccggtat tccaagctca ctttttatct tgccagcggt ctctatcccg tccatccctc
     4321 cttttatcct gatatccatc aatacaagtt cagggttctt tgcccttgct tcataaagcg
     4381 cttccttgcc tgttgacact atgcctgcgc tttcatatcc ttttcttttg attatatttt
     4441 ctatatcctt tgccactatt gcctcatctt caactatcaa gatagatggt ttcatcgttt
     4501 atccctctct tttgcaaatt ctacttcaaa ccttgtccct ttttttctgt atagcttcaa
     4561 cttgccgcca agttgtcttg ccaaggtgac tgcaagctca aggccaaggg tcctgctgtt
     4621 agatggatca aggttaggag gtagtcctat cccgtcatct tctacagtga gtttgtaagt
     4681 tttgcctcta gccctcaggc ttacttttac cttgccttcc cggttgtctg gaaaagcatg
     4741 tttaagggag ttggttatga gttcgttcac taatagccct gcagatattg cctggtccat
     4801 gggtatatgc gcctcttcac agtcaagctt tatcgagacc ctcgggctgc cgcatgtcct
     4861 tccaagatca ttggttaagt ccatcaggta ttttgagaaa tcgatattag aatattcccc
     4921 tgacttatag agcatctcat ggatgagcgc catggacttt atccttccca tgctttctgc
     4981 catggccttg gaaagccgct catcatcaaa ctttctagac tgaaggctaa gaaggcttga
     5041 catcacctgc atgttatttt ttactcggtg atgtatctcc ttaaggagca tctctttttc
     5101 atcaagcgac cttttgagct tcacctctgc ctccttctgg cttgtgatgt ccttggcgat
     5161 gcaggttatg ccgactgtat tggaactctc atcgataagg ggtgttatga accattcaca
     5221 gattatcttc tttccttgct tgctcttgtt ttcccttaca gcatgcactg acatcctgcg
     5281 atgggttatc tttgaccata tatggttctc ctgcttggtg ctgataatct cccacagatt
     5341 ccttccaaga acctcctttt cctcataacc gaaagtattt ctggcgccgg gattccaatc
     5401 ggttatctga aagaaagggt ttagctcgat tatcaccata ggagtgcttg ctttaaggag
     5461 tgaatgcctt ctctccgatg ccctcaggga ttcttctatc ttctttctct cggttatatc
     5521 atgccatatc actacgtcac cgattatgtc ccccctgcca tagataggag aagcagagac
     5581 aaaagctgtc ctcaccaagc ctttcctatc tttgaataag atgctttcgt cacggactgt
     5641 ctttcctgca agggcatctt cagaaatctt gctgatagag gacatgttgg ccttgcccag
     5701 ggatatcact gatcttgcct ctttgtttat ccttattatc ttgtttgtct tgtcgaatac
     5761 gacgacaggg cacctcattg aagccatgac tgcatcaagc ccaaggtctg gttttaagcc
     5821 ggatcttgac atgtcagaaa tcgcttttga cgctctctct tggcatttta acccagggtc
     5881 agccggcgat ttatgagtgt cggaaagctt tgctttctga caaaccagaa atttgcttct
     5941 caaatttctc agttcatgct caaaaactcc atgagcatgc ctagcaattg attgcgaagt
     6001 ttttgacaaa acagcacctt ccataatccc cccttgtgta ctgatttata gctaaataaa
     6061 aaataccaac ctggggtatt ctttaatgtt ggaataccct accaatcaca aatgcaaagt
     6121 atcaacccat tcactgcaag cagtattcca acaaatcaaa aagataatag ctttaaatag
     6181 tttttggtgt attgttttat aaaaatagaa aaaagcgtac tattgctagg tatctacact
     6241 ttgagccttc cctcatgcct aatgccctga cggttagaat ctccccccat tactataaca
     6301 tccagtaatg cagataggtt ccatcatctc atcgacttta tccttgccat gttcatcaga
     6361 ctttccacag taactattcc gatgtacctt cctttccttg tcactggagc ccctttcaca
     6421 tttttctttt ttagctccac caatatatga taggcatcat cttcagaatc gataggcgag
     6481 actgcctgca ttatatcttt gaccttcatg ctccctatat tctgtgcatc attaagcttg
     6541 tcgatatcag cgataaaata ttccctttcc ttgactatcc caaagctcag gtggtttgcg
     6601 acgcaccaat cgaagaattt gcttaacgga aggtccttgt cgatgagtgt ttttttcatc
     6661 acatggccat caaggcttat cttcttgagg ttctctttta tgattagctg ccgatagctt
     6721 tcatttgcaa ggaaaacaag aaaagcacct atcagtatat accatacgct tcctccgccg
     6781 gcaaacatgc ctgcgatgcc aaggaacatc agcatatacc caaaaaggac gcctcccttg
     6841 gaggctatca atgtcgcctt cctgatatct ttctggacag cccacagtat cgaacgaagt
     6901 atccttccgc catccaaagg atatgcaggt attaggttga atactgcaag tatgaagttt
     6961 atcttataga gataatcaaa gatagcagtg agatgcattt caatgtctag agcatataat
     7021 gcaagaaaaa caaggccaag gaccgcgcta aaaagaggcc ctgcagctgc aagtaaaaat
     7081 tcgctcttgg ggcttgtctc ttctgcgctg gcagcagcca cgcctccaaa gaagaacaag
     7141 gttatgcttt ctacctccat gccttccttt tttgacacta tgctgtggga cagctcatgc
     7201 ataagtacag ataaaaaaag gaggagagca gaaaaaaacg ccagcaccca gtcaagggct
     7261 gcagaaaaat cataatactc tggaaaatag aactgtgcaa gtgaataggt tagcagaaaa
     7321 aatatcaaaa accaagttgc atgaagatat atgcctatgc caaaaatcct tcctatccgc
     7381 agcgaagatc tcattggatc ctttcctttt taaaaagcca tacattgccc ttttcaagct
     7441 tctgtaacac aaaatatccc ctcagtttct ctccttcaag tataaggctc aatttgccgc
     7501 tcttcaaggc agcctcgaga tcctgttgga taaggcaggt cccttcatcc caaagcttgc
     7561 agtccaggct gtctgcatgg tccacttcga tagcaagctg tttttgttca gaaggccctc
     7621 gaaatatcgc ccatgacttt atcatgtctt taaagactac tctaaaatca tattgaagca
     7681 tcgatatttc atgtttttgg acgacaaacg ctgctgcttc catatttaca ggtcacttaa
     7741 tttttggtat gaaaatctgc ctaagcttgg tggggatatt gctaagcaca tcctctatct
     7801 cccctctgga tatctttgac tcgataactg taagcactgc ctttgtcgcg tcttcaggct
     7861 ggataggctg ggtggtcctt ttttgaacct cggcatagaa atcctctgct tttttgatcc
     7921 tctttggctt tctgctgggc ttatagcctt cataatacac gcccttcatc agcatcggaa
     7981 ggcctgcagc aaattctgcc gcctcctcga caggcagcct gtcccttaat gtttgcagca
     8041 cggcttttga tgcctcaaaa gccatctggg gattctccca gccaagctcc ttttggatat
     8101 cgttgaacca ttcgtatgtt ttgtcgaggc ttcgttctat tgatcttatc atgatttcac
     8161 ccgatgtacc tttgctccct gtcagacttt gccctggaaa gctggtctat aaacgaccct
     8221 agctcctttt ccatatcctt gcttgcctgc tcaagccgct ctgtgctcac atcaagattc
     8281 aggtagcggt tgatgacctt gacaaggttg gttgcagtta tccctgatgt caagacaggt
     8341 gctgccgcag ccatagcccc aaacttgtcg cttttcatgt ttccaagcaa gcatatgacc
     8401 ggcaggttgc gcttcataag aagggcagat gtgactccgg caacgacgct gttttcaaca
     8461 ggttttgcta tcgattgcag gtccttgcca aagttggata tgtagaagat gctttgatga
     8521 tcctcggcga ttatgccatc cggtattatt atctcttttg ctttcatgct ttttgcaatg
     8581 gtctctatca tgtcagcgat gttccactcg ataccgactg taggagcaag tatagatatg
     8641 aggacgatgt tgttgattga attaaagaat atgcttacag gcttaagtat cctttttccc
     8701 ttgaacacga taagcggagg cacctcccta agctggctgc tgccaatctc cttgaaatcc
     8761 atctcatcta tcagatggtt cacgacaagg gctccgacca tgccaaatcc ttggaatccg
     8821 cagattattg tcggaccaat tatcggcctt tcaaggtgta tcttttctcc gtcctctagt
     8881 ttgatgtctt gtgccactat ttctctctag tccctaagca gtctgaataa tgcaactatc
     8941 gcgacaatag cagttgccgg agcgacgaac acgtttagca gcccaaagaa agtgccgata
     9001 gcttcgtatc caagcagaac cgtcgtaagc acttgtgcaa gagcctgaaa gcttatcaaa
     9061 aatgcgatag cagccatcag gaaaggagtc acttcttcgg cagcgatgtt aaaaagccct
     9121 gctattattc ccagtatccc taggacccaa atagcccagg ttgggatagt tgcagctcca
     9181 aagattgcga tgatcactgc aatagcaagg cctatcaaaa atatgatgcc gttccatctc
     9241 attttttcac ccctaatttt acatcacccc tttaatattt agtgttaaat aactaaattc
     9301 atgtattaat gataaagata acaacataat aaaatcataa tgcttatctt tacaaaatat
     9361 ttaaaccttt ctatattcag aaaagtaaag aataaatttt taaatgaaga acaaggtaca
     9421 ggtgtttatg aagaagattg cacaaggcgc tgaaagtaag atataccgaa aagacgacaa
     9481 aatagtaaag gttcgcttcg aaaaaaaata caggataaaa gagattgatt caagattaag
     9541 gcaggccagg acaagaagag agaaaaagat actagataag ttggcaaaga ttggctttaa
     9601 gtctcctgct ttgatagaaa gcgatgggaa agaaaagatt gaaatgcagt atatcgacgg
     9661 gccaaaagtg agagatattc ttgaaaagag tcaagaatta tcagaggaga taggaagaaa
     9721 ggtagccctg attcacaacc acggcataat ccatggcgac ctcaccacgt ctaatatgat
     9781 atacaacaaa gaggtttatt tcatcgactt tggcttaagc ttcttttctc acaaattgga
     9841 agacaaggca gttgaccttc atctcctaaa gcaggcgctt gaaagcaagc attacaagat
     9901 ttgggagagc tgttttgatg ctgcactaaa agcttatgtc aaggaagcaa aagacggcaa
     9961 agaagtccta aaaaggcttg agatagtgga gaagagagga aggtataagg gtaaaagata
    10021 gttacctact tattaatttc tgatataatc ttaagcagcc agcgcctata tttgttagct
    10081 gataatgttt accattataa gagacttggt tgctggaaat gattgtagcg agttggtcgc
    10141 ccttaatacc tataagacca gtatgctccc acgcacttgt attgataaat cctgctccag
    10201 aaatagcctc tctagcttgt aaatctgctt cattttccga tattgtatct gtcaactctc
    10261 tcttcttttg cgcataaaca gaataagctg cctcatgtct ttgcttaatc tcatcaaaag
    10321 tcaagtgagt atctacagcg atgacaaatt gtatcgactt ggtatgcccc ggatctgtaa
    10381 catagccgat aagcctggcg tttttcaact catggctact aagcccagct tctgttgccg
    10441 cttcggattc aaatctctca aagataggat ttgtatagaa tctctcgctt gcctcctcgg
    10501 gcttcgcaga gattgagccc gatgggacta tacatgcttg tccagcttca acctggcctc
    10561 cccttaaacc gtaaagaaga tagccatctg ctgttcgtat aagtccattg gtcccgatag
    10621 gagtgatatg ttttgccttg ccttcccttg cagcaagaac ttcatagctg gttgtagaat
    10681 caaggtataa tcttctcgga tctacaggat actttccgct tagcgcctcc atatagggcg
    10741 ttagcaatac aagcgcaatc tctttccagt gagagataga actgccatat tgttggtgat
    10801 gtctggcaag gatgctatca aaatcagtta tcttaacaca gcttgctgtt gtctgtgatt
    10861 cactcaaggg ttctttgaac agcactatcc tctgctcaag tccatcggtc atattagaaa
    10921 gtaagtaatt tggcatttat aaatcttatg attattaact actaatatat agtaacttag
    10981 tgataaaaaa ctatataaaa caagcgcata taacaatttt tatgtgggtt gccaaactaa
    11041 agctaaaaca cgatgactgc gtcatcggaa gaagatgcaa aaggttccat gtcatatcga
    11101 taggtatccc gttcaattct tataaggaag ctgacaagca atatttctct cattttgaga
    11161 cattagtagg cgaagaaaac agcatccaaa gattcatcga agatgtcaaa gatgatcctg
    11221 ctatccacag cctggaagtg cagggcagtt ccattttttt catcaatgaa gttcccatcc
    11281 agcatacgat tccaacaacc cactacaacc ctaagatatt cttcataaag ccagtagtgg
    11341 ttgacgaaac aggcagtgaa agctgggaga taggctcatg ggacgagaca gtcttaagaa
    11401 actttatcgt caatctccaa aaagagcatt ttgaggtaaa gatcctaaag ctccagaacg
    11461 aaaagataaa tgagatatat ttccctcaag tcatgccttt cctcaccaag ggtcaaaaga
    11521 gagcgcttga gatagcgacc caaagaggct attatgacta ccccagaaat gttgaactaa
    11581 aggacctggc aaaagaagca ggcatctctc tttcaacatt tagggagcac ttaagaaaag
    11641 cagaaaaaaa gataatgcct gacttgataa ggaatgtcag ggatgaatga ttatatcttt
    11701 tgatacttcc ccatcagctc tgcctgcgcg attgtatgca ggttcacctg ctggtaaagc
    11761 tcagtcttgt cttcagcagt gagtttttct acatcaagcg caaagagctt gaaaaaatac
    11821 ctgtgcaaag caggcggaca tggcccccca taattttccc tgccgaatga attcttaagc
    11881 tgcactccag ggatgctatc ctgaggtatc tcccgcacgt cagcaggtat atccttaacc
    11941 atccaatgga tgaaattgcc agacgcagca tcaggatcgt ctatgacaag cgcaaagctc
    12001 attgtccctg caggaacatc ttcccaggaa agctgcgggt tgatgtcctc tccctggcat
    12061 gtaaactttt tggggatagt atctccttgc ttgaaatcat cgctcataag tttcatctta
    12121 ccgacctccg tattattctc tatcttatcg acattctgct ggccttggca gcctaatacc
    12181 aaagcaagta caaaaaatat cacgaaacgc ataataagtg ccaaggatta aaacgttaat
    12241 aaattttgct aaaatttatc aataaatata tattcacagg gttatgtcca taaattatga
    12301 atctcttctt tatgacagat atgaaataac cgcaatattg ttcgtacgca ttgcagatca
    12361 ttggtctgtt gttcagaaag caaactctgc tgaaactcat ggatgctgtc ctcttgagtg
    12421 gagaattaac gaatctctta agcctaagaa cccaaaaaaa acaatgattg gtaaaagaat
    12481 gccgccggag agcttttttt tagatgcaga catccattta caaagagata aatcattagc
    12541 agacatgcat tgctgcctcg aggttgacaa agatggctta tgggcacatg atctatcgac
    12601 ttatgggatt gcaggcaagg attatcaggg aaatacttat agcaatatta atgcaccaca
    12661 gaaaccgata aggcaacttc ttgtcccagg taatattgaa gaaaaaagtg caaggtgctt
    12721 tatctctttt gggcattatg cgctaaggtt tgatctaaag atgacagaac catcgaatca
    12781 tcaaaaaatt tataaataac tcatcctaac tgaaaatatg gtggcagaca actacacaat
    12841 agctacttta ggttctcatt ctgctctgca gattttaaaa ggagcaaaag atgaagggtt
    12901 caggactgta tgcatctgca taaaaggaag ggaaaagctc tacaagcagt ttaaggttgc
    12961 agacaagata atcattatca acaattatgg cgagcttgaa aatatccagg acgaattgct
    13021 aaaagaaaat cctatcctta tacctcatgc ttcctttcta acctatatgg gctttgaaaa
    13081 agttgaacag ctaaagatcc catacttcgg aaataaaaag atcctaagat gggagtcaga
    13141 caggaacatg gaaaggaaat ggcttacaga agcagggcta aagactccga tgattttcca
    13201 gaagcctgaa gatatagaca ggcctgtcat cgtcaagttc cacggagcag acggcggcaa
    13261 aggatatttc cttgcaaaaa acacaagaga cttccgaaag aagataaagc ttcatccagg
    13321 aaaaggctac gtcatccagg aatatatcct tggagtccat atgtacgctc attatttcca
    13381 ttcaccattg acagaagaga cagagataat gggctttgac aaaaggtatg aaagcaacgt
    13441 ggattcgctt ggaaggatct cagcaagaga tcagatggcg cttccaaaag agaaagttga
    13501 ccccagctat gtcattgtcg gaaacatccc tatagttgtc cgggaatctt ttctccaaag
    13561 attcatagaa aaaggagaaa acgtcgtcaa ggtcagcaaa aagatagccc cgccagggct
    13621 ctttggccca ttctgccttg agacaataat gactccagaa gaggatataa tcacatttga
    13681 gatatctgca aggatcgttg ccggaacaaa tccctatatc catggctctc cttattcgtg
    13741 gcttaagtat gatgagccta tgtcaacagg cagaaggata gcacgggaga taaagcttgc
    13801 aatagagcaa ggccagttaa agaaggtgct tggatgaaaa aaggagtctt tcaccatctg
    13861 ttcaaagggg tccagataga agtgagcagg gtagaaagaa atgaagcaaa ggactcaggg
    13921 aaaaaacagg cagaaaatcc ttttgcccat cttgaaagaa agccattgaa gataagctca
    13981 ggaacaaaga tatcagaaat acttgatcat tatgataagc gtgctataac gataggcgct
    14041 cttggcggcc attctgcctt ggatgtatgc agaggcgcaa aaaaatatgg atttaggaca
    14101 gtcgttgtct gccagaaagg aagggaggca acatacgcaa aatattacaa ggcaagggat
    14161 agcggtaagg ggattgtcga tgaagttata cttgtcgaca agtttagcga tataacaaaa
    14221 aaagatgtcc aacaaaaact tagggactta aataccatat ttgtccataa cagatatttt
    14281 tgggtctatt gcaatttcaa ggatatagag aaaaacttta aggttccaat ctatggcaca
    14341 agaagtatgg taaagcttga agagcgtgac cagccaaaaa accaatatta tcttttaagc
    14401 aaagcaggag taaggatgcc caagctcttt
//
LOCUS       DASRUK010000122         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1611965, whole genome shotgun sequence.
ACCESSION   DASRUK010000122 DASRUK010000000
VERSION     DASRUK010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611965"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VFF28_05470"
     CDS             <1..883
                     /locus_tag="VFF28_05470"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HZX45103.1"
                     /translation="MITTPDYGNGEPLPDTEEKIASHLRKVTEVPVITGFIGKDSAGN
                     ITTLGRGGSDYTGAIIGAAMNDTIEIWTDVNGVMTSDPRVVNEAATIENISFDEASEL
                     AYFGAKVLHPKAIWPAIKKNLPVKVLNSFEPTNKGTTIVSKSKESKTVIKAIACKKNI
                     SLINVSSTRMLLAHGFLSRLFSVFEDYKKSVDMIATSEVSVSLTVDTEENIEKIREDL
                     EGVAEVNFEKGKAIVAIVGAGMINSKGVVGRVFTALKDIRVEMISAGASEINISFIVD
                     NKDADRAVQTLHKEFFG"
     gene            complement(884..1273)
                     /locus_tag="VFF28_05475"
     CDS             complement(884..1273)
                     /locus_tag="VFF28_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HZX45104.1"
                     /translation="MIKLSLGEIAKALDEIKLPHFDLLVAIGSDGIKPAQILQKRLTI
                     PLEIVWINYRGSNNMPIRDEPELTKPFSGKLAGKKILLVDTVARTGKTLEKAKEILGG
                     NEIKTFVINGKADYSLFDYKECIEWPW"
     gene            complement(1266..1736)
                     /locus_tag="VFF28_05480"
     CDS             complement(1266..1736)
                     /locus_tag="VFF28_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45105.1"
                     /translation="MNYWPGFEINRKSLVLLSILAILPNFLGLINLSLFGIKLHFFQY
                     AIFLAAIVYGPAGGFISGAIGSVYIAMTLNNPYIILGNVILGFSFGVFVRLKWNAVLA
                     GLTAFAIQVPWLWITDVYLMKMPTNLVIGIIIALFLSNIVWSFAADKSKRYFYD"
     gene            complement(1733..>2466)
                     /locus_tag="VFF28_05485"
     CDS             complement(1733..>2466)
                     /locus_tag="VFF28_05485"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HZX45106.1"
                     /translation="ALKKAQELGFAEANPEFDVEGKDAAQKLVILAELAFNSRISDEI
                     FTWGITKISANDIKYAEQLGYKIKLLAIGKKENGEIELRVHPTMIPKNHELASVKNEF
                     NAIYTVAENTGKTMLYGKGAGQLPTATVVLGDIIDAAYNKKQTFLMEEAKIKDINKIT
                     SRYYLRFNVEDKPGVLAKIAKILGDNQISISGVEQKEMNKSIVPIVMITHKAVEDNMM
                     KAIKQANGCDFVKEPGVVIRIEDIG"
BASE COUNT          732 a          531 c          481 g          722 t
ORIGIN      
        1 tatgataacc actccagact atggaaatgg agagcctctt ccagatacag aggaaaagat
       61 agctagccac ctaaggaaag tcactgaagt tccagtcatc acaggattta tcggaaaaga
      121 cagcgcagga aacataacca ccttaggcag aggaggctct gattatacag gagcgattat
      181 cggagcagcc atgaatgaca ccattgagat ctggactgat gtcaacgggg ttatgacatc
      241 tgatccaagg gttgtaaatg aagctgcaac catcgagaac atatcttttg atgaagcatc
      301 agagcttgca tatttcggcg caaaagtttt gcatccaaag gcgatatggc cggcaataaa
      361 gaagaacctg cctgtaaagg tattaaactc gtttgagccc acaaacaagg gcacgacgat
      421 agtcagcaag tcaaaagaaa gtaagaccgt cataaaagct attgcttgta aaaagaatat
      481 cagcctgata aatgtcagct ccacaaggat gcttctggcg cacggttttc tgtcaagact
      541 attcagcgtc tttgaagact acaaaaaaag tgttgatatg atagcgacat ctgaagtatc
      601 ggtcagcctc acggttgaca cagaagaaaa cattgaaaag ataagagaag accttgaagg
      661 cgttgctgaa gttaattttg aaaaaggaaa agcgattgtg gctatagtcg gcgcaggtat
      721 gataaactca aaaggcgttg tcggaagggt atttactgcc ctaaaagata tcagggtaga
      781 gatgatctca gccggcgctt cagagataaa catcagcttt atcgtcgaca acaaagatgc
      841 ggacagggca gtgcagactt tgcataagga attctttggc tagtcaccaa ggccattcta
      901 tgcattcttt atagtcaaac agagaataat ctgccttgcc gttgatgaca aaagttttaa
      961 tttcattacc tcccaatatt tcttttgctt tctctagggt ctttccggtt cttgccacag
     1021 tatcgaccag caaaattttt ttaccagcta attttccaga gaatggcttt gtcaattctg
     1081 gctcgtccct tatcggcata ttattgctgc ctctgtaatt tatccaaaca atctcaagtg
     1141 gaattgttaa tctcttctgc aatatctgtg ctggttttat tccatcgctt ccaatagcta
     1201 ccaataagtc aaaatgaggc aattttatct catccaatgc ttttgcaatc tctcccaaac
     1261 tcaatttaat catagaaata ccttttgctc ttatcagcag caaaagacca gacgatatta
     1321 gacaaaaaca aagcgattat aatcccgata accaggttag taggcatttt cataagataa
     1381 acatccgtta tccacagcca agggacttgt attgcaaaag ccgtcaagcc agcaagcaca
     1441 gcattccact taagccttac aaaaacgcca aaagagaaac cgaggatcac attacctaat
     1501 attatataag gattgttcaa cgtcatagca atatagacag aacctatagc accagatata
     1561 aatcctcctg ccggcccata tactatcgcc gcaaggaaga tagcatactg aaaaaaatgt
     1621 aattttattc caaacagtga aagattgata agtccaagaa agttggggag tattgcaaga
     1681 atgcttagta acacaaggct ttttcgattt atctcaaaac caggccaata attcatccga
     1741 tatcctctat ccttattacg acaccaggct ctttgacaaa gtcgcagcca tttgcctgtt
     1801 ttatggcttt catcatatta tcttcaactg ctttgtgggt tatcatcact attggaacga
     1861 tagacttgtt catctctttt tgttcaacgc ccgatatgct tatttggtta tctccgagga
     1921 ttttggcaat cttagcgagc acccctggct tgtcttcgac attaaacctc agatagtatc
     1981 tggaggttat cttattgata tcctttatct ttgcctcttc cataaggaat gtttgctttt
     2041 tattgtatgc tgcatctatt atatctccca gcactacagt tgcagtcggc aattgccctg
     2101 ccccttttcc atatagcatt gttttgcctg tattttcagc gacagtgtat attgcgttga
     2161 attcgttctt gacagaggca agctcgtggt tctttggtat catagtagga tgcactctaa
     2221 gctctatctc gccgttttct ttctttccta tagccagcag ttttatctta tatcccagct
     2281 gttcagcgta ttttatgtca tttgcgctta tcttggttat gccccaggtg aaaatctcgt
     2341 cactgattct ggagttgaat gcaagctcag caaggatgac cagcttttga gctgcatctt
     2401 tgccttcgac atcaaactca gggtttgctt ctgcaaatcc aagctcctgc gcttttttca
     2461 aagcat
//
LOCUS       DASRUK010000123         2868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1922876, whole genome shotgun sequence.
ACCESSION   DASRUK010000123 DASRUK010000000
VERSION     DASRUK010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1922876"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VFF28_05490"
     CDS             <1..633
                     /locus_tag="VFF28_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1297 domain-containing protein"
                     /protein_id="HZX45107.1"
                     /translation="VSEAARGYERAFFFCNSEESYNKKSEELLSKGIITKEALKKAVI
                     EEYIIGAQVNFNYFYSPLTGELELMGTDTRRQTNLDGLLRLPADIQLEVLKYLKPKII
                     ETGHIAVTTKESILEKIFKIGEQFVETCKKEHPPGIIGPFALQGAVAAEEGKEELVIF
                     DVSLRIPGSPGTLFTPYSGYLYHDSISYGERIAMEIKQAIEDDKLDWICT"
     gene            complement(638..1042)
                     /locus_tag="VFF28_05495"
     CDS             complement(638..1042)
                     /locus_tag="VFF28_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45108.1"
                     /translation="MATLYQQQVSALQRNNFAAKGGTYRHYQKDESLEGKTYSFGVDL
                     NRSGSFDLSVRANSSIPVEPESLGEAVNAYDLYISQMSRCIKRSFGKDAQICATPPHT
                     LGVNAIYISGRMDVGEDYLKLKEYIQKKGVVV"
     gene            complement(1082..2614)
                     /locus_tag="VFF28_05500"
     CDS             complement(1082..2614)
                     /locus_tag="VFF28_05500"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010042607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HZX45109.1"
                     /translation="MSGEVKDECGIAAVSLKKEKENNALFYIYRLLLNLQNRGQLSAG
                     VTTYNKEKAQLLSTYRNIGTVNEVFKTSDIQKSMQIFKNYSGIKGIGHVRYATFGAND
                     RSYAQPFERKHGRLWKWFSFCFNGNLVNFAELKRPLVEKTEYHFILNNDTEIIQHYLA
                     RELKGNKKPDVSSVFHNLSKKFDGAYNIAYMDAYGDVVVSRDPLGFRPLVYSIKDGNL
                     FAASESNALLNIGVYDFKSLKPGELIHVSNGNISVKRYAKSKRRAHCMFEWVYFANVG
                     SVLDSRSVYVTRTRLGKELAKLETENITDEYIVVPVPDTAKAAGDAYAYELGVPSMEG
                     LIRNRFVGRTFIEGSKREDKVASKYTVIKEILRGKKVLLVDDSIVRGTTTNQIVKFLK
                     TVGGAKEVHIRATCPPIIAPCFYGIDMSTVGELLVPRYTQQEGQIPQQVFDKIASDLE
                     ADSLIYQTIPGLIKSIGLSGGDLCLGCLTGKYPTECGKATYCRAIKEFKIGRENQRSY
                     EH"
BASE COUNT          798 a          651 c          585 g          834 t
ORIGIN      
        1 gtgtcagagg cagcaagggg atatgaaagg gcatttttct tttgtaatag cgaagaaagc
       61 tacaacaaga aatctgaaga gctcttgtcc aaaggcataa tcacaaaaga ggctttgaaa
      121 aaagcagtca tcgaagaata cataatcggc gcccaggtca acttcaatta tttctattca
      181 ccattgacag gagagcttga gcttatgggc acagacacta gaaggcagac aaatcttgac
      241 ggcctcttaa ggctccccgc cgacatccag cttgaagtgc tgaaatacct aaagccaaag
      301 ataatagaaa caggccatat tgcagtaacg acaaaagagt cgatattaga aaagatattc
      361 aagataggcg agcagtttgt ggaaacatgc aaaaaagagc atccgcctgg aataataggc
      421 ccctttgcct tgcagggagc agtcgcagca gaggaaggaa aagaagaact ggtcatcttt
      481 gacgtctcat taaggatacc tggcagccca ggaacattgt tcacgccata ttcaggctat
      541 ctataccatg attccatctc ttatggtgaa aggattgcaa tggagataaa acaggcgata
      601 gaagatgata agctcgattg gatctgcact taagccttca gacaacaacc cctttctttt
      661 gtatatattc ttttagctta agataatcct ctccaacatc cattcttcct gatatataga
      721 tagcattaac tcctaaagta tggggaggtg ttgcacatat ctgagcatcc ttgccaaatg
      781 atcgtttgat gcatcttgac atctgagaga tatatagatc atatgcatta acagcttcac
      841 caagagattc aggctcgact ggaattgagg agtttgccct cacacttaag tcaaacgagc
      901 cgcttctatt taggtcaacc ccaaatgagt aggtctttcc ttcaaggctt tcatcctttt
      961 gataatgcct atatgttccg ccttttgcag caaaattgtt tctctgtaaa gcacttactt
     1021 gctgttgata caatgttgcc atcaaaaaaa caacttaaga attgatatat aaaccaaatc
     1081 tttaatgctc gtatgatctt tggttctctc tgcctatttt aaattccttg atagctctgc
     1141 aataggttgc ctttccgcat tcggtcggat acttgcccgt aagacatcca aggcaaaggt
     1201 ctccgcctga aagcccgatg cttttgataa gtccagggat agtctgatag ataagtgaat
     1261 ctgcttctag gtcgcttgcg atcttatcaa agacctgctg aggtatctgc ccttcttgct
     1321 gagtatatct tggcaccaaa agctctccaa cagtcgacat atctatgcca tagaagcatg
     1381 gcgctatgat tggtggacag gtggccctga tatggacctc ttttgccccg ccgactgtct
     1441 ttaggaattt tactatctga tttgttgttg tccctctgac tatggagtca tccacaagaa
     1501 ggaccttttt tccgcgaagt atctccttga tgactgtata tttgcttgca actttatcct
     1561 cgcgcttgct tccctcgata aatgtccttc cgacaaacct gttcctgata agcccttcca
     1621 tagaaggcac gccaagctcg tatgcataag cgtcgccggc agcctttgca gtgtcaggca
     1681 ctgggacgac gatatattca tcagtgatat tttcagtttc aagtttagca agctctttgc
     1741 caagccttgt ccttgtgaca tacacggatc tgctgtcaag gacgcttccg acattggcaa
     1801 aatataccca ctcgaacatg cagtgggctc ttcttttaga ctttgcatac cttttgacgc
     1861 tgatattgcc gttggaaaca tgtatcagct ctccaggttt aagagactta aaatcataga
     1921 cccctatatt taaaagggcg ttgctctctg acgccgcaaa caagttgccg tcttttatcg
     1981 aatatacaag cggcctaaat cccagtggat cccttgagac cacaacatct ccgtaagcat
     2041 ccatatatgc gatgttgtat gctccatcga acttcttgct taggttatga aacacgcttg
     2101 aaacatccgg ctttttattg cccttcagtt ccctggcaag ataatgctgt atgatctcag
     2161 tatcattgtt aaggataaag tgatactctg tcttttccac aagtggcctt ttgagttcgg
     2221 caaaatttac caggttgccg ttgaagcaaa atgagaacca tttccaaagc cgtccgtgtt
     2281 ttctttcaaa aggctgggca tagctcctgt cgtttgcccc aaatgtcgca tacctgacgt
     2341 gcccgattcc ttttatgcca gaataattct tgaatatctg catgctcttt tgtatgtcgc
     2401 ttgtcttgaa cacttcattg acagtcccga tattcctgta agtgctaaga agctgtgctt
     2461 tctctttgtt ataggtagtg actccagcag aaagctggcc cctgttctga aggttgagaa
     2521 gaagcctata gatgtagaat aaagcgttat tttccttttc cttcttcagc gaaactgctg
     2581 cgatgccgca ttcatctttt acctcaccgc tcatcaaaac aaataactcg agctcacttt
     2641 ataaacatgt cgaaaaggag ggacatagcg atagcacttc aacaatcttt atatataacc
     2701 ggggttagat gcagcttagg atgagcggta tatttggggt ggtcagcaga gacaactgta
     2761 tctctacgct cttttacggc acagattacc atagtcatct tggaactcaa tttggaggta
     2821 tagcttatat caatccagac ggcaagcctt caaaaaagat tcacagca
//
LOCUS       DASRUK010000124         3801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2429394, whole genome shotgun sequence.
ACCESSION   DASRUK010000124 DASRUK010000000
VERSION     DASRUK010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2429394"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(355..882)
                     /locus_tag="VFF28_05505"
     CDS             complement(355..882)
                     /locus_tag="VFF28_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HZX45110.1"
                     /translation="MRLTDDLIEEHKLIKKLIGFLSDKAKLIEEGSKADKDFFEKAVY
                     IIKNFADKFHHAKEEDILFVEMVKKGMPEKDSPIEAMLIEHEQGREFVSGMALALERL
                     DKDAGQAIIQNARGYIELLNEHIDKEDNILYPLAERMFTQAEKQKMLKEFKRAERGKL
                     PFKEYQAMIGSLERR"
     gene            complement(879..1559)
                     /locus_tag="VFF28_05510"
     CDS             complement(879..1559)
                     /locus_tag="VFF28_05510"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HZX45111.1"
                     /translation="MAVLIATNSPLEGPGYIGSALQHLNIKHDVVSSKARVDISRYSS
                     FIMLGGPQSVNDDTEEIKKALKLVRDITDSGKPYLGICLGAQLLAKSLNTRVKPNKVK
                     EIGSHIVTLTDEGRKNPLFSGLPPTIKVFQWHGETFEMPNGAKRLAEGSLCQNQAFQH
                     GNCYGLQFHLEVSRDMVKEWLFNDGYKSELKEQGIDIFQFAERFAREEEEYESSCRIL
                     INNFVVQK"
     gene            complement(1571..2020)
                     /locus_tag="VFF28_05515"
     CDS             complement(1571..2020)
                     /locus_tag="VFF28_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding oxidoreductase"
                     /protein_id="HZX45112.1"
                     /translation="MEFCGCPGSAMKDFGKKRESKASTGRVDNELRQWPIQLHLVSPS
                     APYFNDADLVIAADCVPFAYANFHADFLKGKPVVIGCPKLDDTESYADKIAQIIREGN
                     VKSITVAHMEVPCCFGLQNIAEEAVSKSGKKLPINEVTITLQGEKQH"
     gene            complement(2022..2237)
                     /locus_tag="VFF28_05520"
     CDS             complement(2022..2237)
                     /locus_tag="VFF28_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45113.1"
                     /translation="MIPENLGEMAIKDVIVKHPKIGKILEKHEIGCVTCKVGICKLKD
                     VVRIHYLPKEKEEVIFREIDQTIQGGD"
     gene            complement(2234..2650)
                     /locus_tag="VFF28_05525"
     CDS             complement(2234..2650)
                     /locus_tag="VFF28_05525"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HZX45114.1"
                     /translation="MEKSLTALFVFDHKRIFELINDLKAEKGREAQHKIFQKIDASLR
                     DHLKEEEQLYSRYHQSTGELLPVFSSLKREHELFLHYLEDIKRSLERGIKAETKIFFS
                     LLERHKNVEERLVYPEFDRVLSKKEKEEHYAMVRDR"
     gene            2735..3082
                     /locus_tag="VFF28_05530"
     CDS             2735..3082
                     /locus_tag="VFF28_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZX45115.1"
                     /translation="MDCTIYKTLELIGSKWSLLIILSIYKTREKRFSGIKKDLKEITS
                     KILSTRLKELEKEGLVKRRIDASTNPISVYYSLTQSGFALTKIIQDVKNWGLQYKFKD
                     KECQAAFCKNCID"
     gene            complement(3095..3439)
                     /locus_tag="VFF28_05535"
     CDS             complement(3095..3439)
                     /locus_tag="VFF28_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45116.1"
                     /translation="MYIPKKYGESRVDKCPFCGRQSICTNKQGIPVCANHKDSKLGEM
                     RCICGGHADMMTGKFGIFFNCLNCGTMNARKIFEINKVEDVNPKTSEQKKVERKAPRE
                     ITVRSDDPRYFS"
     gene            3514..>3801
                     /locus_tag="VFF28_05540"
     CDS             3514..>3801
                     /locus_tag="VFF28_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HZX45117.1"
                     /translation="MLELRKDYILDRWVISASVRDQRPKQFKITHEKVEPKLCVFCPE
                     NINLTPSETGRVEKNGKWILRWFANKFPFVEQKGNPKLRKTGKFLVNSAGYG"
BASE COUNT          965 a          881 c          721 g         1234 t
ORIGIN      
        1 gagacaccgc agccaatgca atgcaacccg tgcttaagca tcacatctgc agaccttgga
       61 tatttgtcta ttacacgaaa aaagccagaa agctccatgc tttagcattg gagatgtgtc
      121 agaaatcgcc aggtcatatt agttgcgatt tctgagcacg ctcaaaaact ttgtttttga
      181 cgaatggctt tctggctttt ttgtgtccta gaaatttaga tggcaagtgc cgatcaagcc
      241 ccacccttca gggttggggt aggcacttgc cctataattt ctatgacttc tccaagatac
      301 atgtttttgc tgacttttgc catttttatc cctccgtata tgtgattact ttgttcaccg
      361 tctttctaat gatcctatca tagcctgata ttctttgaaa ggaagttttc ctctctctgc
      421 ccttttgaac tcttttagca ttttttgctt ttctgcttga gtaaacatcc tttcagcgag
      481 cggatagagg atattgtctt ctttgtcgat atgttcgttg agaagctcta tataacccct
      541 ggcgttctgg atgattgcct ggccagcatc tttatctagc cgctcaagtg caagcgccat
      601 cccacttaca aattctcttc cctgctcatg ctcgataagc atcgcttcta tggggctgtc
      661 cttctcaggc atgccttttt tgaccatctc gacaaacagt atatcctcct ctttggcgtg
      721 atgaaattta tcagcaaaat tttttattat gtaaacggct ttttcaaaaa agtccttgtc
      781 tgctttactg ccctcttcga ttaacttagc cttatcgctt aggaagccaa taagtttttt
      841 tatcagctta tgctcttcta ttagatcatc tgtaagtctc atttttgcac cacgaaatta
      901 ttgataagta tccggcagct gctttcatat tcttcctctt cccttgcaaa cctttcggca
      961 aactggaaga tgtctatccc ttgttcctta agttcgctct tatatccgtc gttaaagagc
     1021 cactccttta ccatgtccct gctcacttca aggtggaatt gaaggccata acaattgcca
     1081 tgctggaatg cctggttttg gcacaggctt ccttctgcaa gtctttttgc gccattaggc
     1141 atttcaaaag tttctccatg ccactgaaag accttgattg tcggaggaag ccctgaaaat
     1201 agcgggtttt ttcttccttc atctgttaaa gtgactatat ggcttcctat ctccttcact
     1261 ttgtttggct ttactcttgt attcagtgac tttgcaagca gctgggcgcc aaggcagatg
     1321 cctagataag gttttcctga atctgttatg tcccttacca gtttgagggc tttttttatc
     1381 tcttctgtat catcgtttac actctgcggc cctccaagca tgataaatga gctatacctg
     1441 cttatatcca cccttgcttt gctgcttact acatcatgct tgatgttgag atgttgaaga
     1501 gcgctcccga tatatcctgg cccttccaaa gggctatttg tagctatcaa caccgccacg
     1561 gccctcacct ttagtgttgt ttttctccct gcagggttat ggtcacctca ttgataggca
     1621 gcttctttcc tgacttgctt accgcttcct cagcaatatt ctgcagtcca aagcagcaag
     1681 gtacctccat atgcgcaaca gttatgcttt tgacattgcc ttccctgatt atctgagcta
     1741 tcttgtctgc atagctttca gtatcatcaa gtttgggaca tccgataact acaggcttgc
     1801 cttttaaaaa atcagcatga aagtttgcat aagcaaaagg cacacagtct gcagcgatga
     1861 ctagatctgc atcgttaaaa tagggtgcgc ttggagatac aagatgcagc tgtatcggcc
     1921 actgcctgag ttcattatct acccttcctg ttgatgcttt gctttccctc tttttaccaa
     1981 agtccttcat cgcagaacca gggcaaccac aaaattccat attaatcgcc tccttgtatt
     2041 gtttggtcta tctctctaaa tatcacttcc tctttttcct ttggaagata gtggattctc
     2101 acaacatctt taagcttgca gatgccgact ttgcaagtga cgcatccgat ctcatgcttc
     2161 tctagtatct tgcctatctt cggatgcttg actatcacat cctttatcgc catctctcca
     2221 aggttttctg ggatcatcta tctctcacca tagcataatg ctcttctttt tccttcttag
     2281 aaagcactct gtcaaattca ggatagacaa gcctctcttc aacattctta tgcctctcaa
     2341 gaagcgagaa aaaaatcttg gtctctgctt ttatccctct ttcaaggctt cgcttgatat
     2401 cctcaagata atggaggaac agctcgtgct ctcttttgag gctcgaaaac acaggcaaaa
     2461 gctcacctgt tgactggtgg tatctgctat aaagctgttc ttcttctttg agatgatctc
     2521 taagagatgc atctatcttt tgaaagattt tatgctgtgc ttcccttccc ttctctgcct
     2581 tgagatcgtt tatcagttcg aagatccttt tatggtcaaa aacaaataag gcagtcaggc
     2641 ttttttccat tgtgtgctca tcccttgata tattttatat actaagtata taatagctac
     2701 ttatttatat aagtgcagta tcctttttga taccatggac tgcacgatat acaagacact
     2761 tgaattgata ggcagcaagt ggagcctgct gatcatttta agtatataca agaccaggga
     2821 aaaaagattt tcagggataa agaaggactt aaaagagata acctcaaaga tattgtcgac
     2881 aaggctcaag gagcttgaga aagaagggtt ggtaaaaaga aggatagacg catcgaccaa
     2941 tccgataagc gtctattact ccttgacaca aagcggcttt gcgctcacaa agataatcca
     3001 agacgttaaa aactggggct tgcagtataa gtttaaggat aaagaatgtc aggcggcgtt
     3061 ttgtaagaac tgcatcgatt gagaggtaaa cattttatga gaaatatctg ggatcatcag
     3121 accttacagt tatctctctt ggcgcttttc tttcgacctt cttctgctca gaagtctttg
     3181 gattaacatc ctcaaccttg tttatctcaa atatctttcg agcgttcatg gtgccgcagt
     3241 taaggcagtt gaagaatatc ccgaacttgc cagtcatcat atcagcatgc ccgccgcata
     3301 tgcacctcat ctcaccaagc tttgaatcct tgtggtttgc gcagacaggg atcccttgct
     3361 tgtttgtgca tattgactgc cttccgcaga acgggcattt atcaactctt gactcgccat
     3421 atttctttgg gatgtacata ctaaaagacc aaatggcagg tgttaatata gctttgcata
     3481 acctttatat atttctgctc aaggaattca cctatgctag agctccgcaa agactatatt
     3541 cttgaccgct gggtcatatc agcctctgta agagaccaaa ggccaaaaca gtttaagata
     3601 actcacgaga aagttgagcc aaaattatgc gtcttctgtc ctgaaaatat caatttgaca
     3661 ccatcagaaa ccggcagggt tgaaaagaat ggcaagtgga tcctcagatg gtttgccaac
     3721 aaatttcctt ttgttgagca aaagggaaac cccaaattaa ggaaaacagg gaagttcttg
     3781 gtaaattctg cgggctatgg a
//
LOCUS       DASRUK010000125         6683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2113082, whole genome shotgun sequence.
ACCESSION   DASRUK010000125 DASRUK010000000
VERSION     DASRUK010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 6683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6683
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2113082"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..715
                     /locus_tag="VFF28_05545"
     CDS             164..715
                     /locus_tag="VFF28_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45118.1"
                     /translation="MVEQMYLADFQKYLSGSGLNLKMDVLKDGPGTIGISCLTLEQRA
                     GDPILSLEPRQNHKGYQLEVIIYTDGSGNNFLPIAGGEGRAKMEYGNDHSRSVHALLE
                     REEIPDLTLTPLGSGASAGRREEQRDRVHKRIQENGYRLYSFYTTKLNGRAAFVVHMP
                     LDPYDAVKKVAGWTRELASLKTA"
     gene            complement(698..1645)
                     /locus_tag="VFF28_05550"
     CDS             complement(698..1645)
                     /locus_tag="VFF28_05550"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HZX45119.1"
                     /translation="MKKLILALLLLAGCTAPLDKYEESKSVMNTTFTITVYDTDAQRA
                     QAGIESAFKEMGRIESLLSHTLKTSEVYRLNAEGEIESFSDDLYYNMEKAIYYSNLSD
                     GSFDITIQPILDLYTKTFSEEKRPPTKEEVNEELKKVGYKNIEFSRDKIKLQPGTKIT
                     LGGIAKGYAVEKASEVLRKDGIEHALINAKSSIMAVGNKGDDDWAIALQNPRDAIEYI
                     SILKLNGNAISTSGDYERFFDDSKKYHHIVNPKTGYSAEELISVTIIADSAFDADALS
                     TAVFVLGKEKGMELIEKLPSVEGLIITGDKKIIKSSGFQ"
     gene            1727..2368
                     /locus_tag="VFF28_05555"
     CDS             1727..2368
                     /locus_tag="VFF28_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HZX45120.1"
                     /translation="MLDGVKNHRVVKGIHFYSFGLLRRLYNWTLSWADTKYGTLALFI
                     LAFMESSFFPIPPDILLIALCISIHKKSFKYAAICTVGSIIGGMFGYIIGYGLYESVG
                     AWIIEALHYQPYFDAVGKMYAGNAFVAIIAAAVTPIPYKVFTIAAGVWKINFWTLVFA
                     SIVGRGFRFFLIASLIYFFGARIKTFIDKYFNLMVTLFLGLLVGGFLVIKYLV"
     gene            2435..3742
                     /gene="rsxC"
                     /locus_tag="VFF28_05560"
     CDS             2435..3742
                     /gene="rsxC"
                     /locus_tag="VFF28_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HZX45121.1"
                     /translation="MIGGIHPEYNKLTADSKIVTAPAPKKVVLPLQQHIGAPCEPRVN
                     VGDIVRSGQKIAEGKGFVSAPIHSSITGKVTAIGSARHPVLGEFNAITIEVVPGLELT
                     EPEKKLRNPEDLNKDELKAIIKDAGIVGLGGAAFPTHVKLSPPPEKKIDTLIINGAEC
                     EPYLTCDHRIMIEEPEKILKGLKVIMKILEPINVYIGIEDNKDDALIVMQLKLKELGL
                     GGKVKIAKMQTVYPQGSEKNLIQSITKREVPNAGGLPMDVGVVVQNVQTVKAIYDAVY
                     EGKPLIERVVTITGAVKEPKNILVKIGTNAKELIDFCGGYDGAVGKVISGGPMMGIAQ
                     PDDNMPVIKGSSGVLVQLEDKTSVAERDCIRCGRCVDVCPVKLMPTMIYQYTRKEDYD
                     GANDYFAMDCFECGSCSFICPSKIPLTHWVKVAKSELMKRKKK"
     gene            3739..4671
                     /locus_tag="VFF28_05565"
     CDS             3739..4671
                     /locus_tag="VFF28_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HZX45122.1"
                     /translation="MTLTVSSNPHLRDKDYVNKIMWTVILALLPASAAGVYFFGMRAL
                     TIIIVSMAFAMATEAIIQWLTKKQITIADGSAALTGLLLALVISPLVPWWVAAIGAVF
                     AIAIGKMVFGGLGNNIFNPALVGRAFLVASFPAIMTSWLIPDGITSATPLGLLKEQGI
                     KTGYLQLIVGNIGGSIGETSAIALLLGAAYLFYKKVIDWRIPAAYIGATFVFTLVFGQ
                     DPIFHVFAGGLLIGAFFMATDYVTSPLTARGKLVFGLGCGLLTGLLRLFSSYPEGVMF
                     AILLMNAAVPLIDRYTKLKPLGYRKVIKSEGPTK"
     gene            4742..5404
                     /locus_tag="VFF28_05570"
     CDS             4742..5404
                     /locus_tag="VFF28_05570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HZX45123.1"
                     /translation="MNHILKLGLTLMLVAVIAGAALSLTNRFTLVQIENQKQNAIKES
                     LNIVIQAESFEEKEGYYEALDKDKKVIGKVLKVNTPGYSSTIQALVGIDLENKITGID
                     IVSQQETPGLGAKIEGEGFLSQFKGKTSTDIKLRKDSGKIDAVTGATISSRAISNGVK
                     ASMEQYGFAAQTDDITGASKPANNTAAKNMTAIPNTITRVNNSAAKKAMPSSIGANNA
                     TR"
     gene            5391..6011
                     /gene="rsxE"
                     /locus_tag="VFF28_05575"
     CDS             5391..6011
                     /gene="rsxE"
                     /locus_tag="VFF28_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HZX45124.1"
                     /translation="MPQGNWWELVKGFFKENPTFAIVLGLCPTLAVSTSLDNAIGMGG
                     AATFVLIGSNLIISAIRKKVPGEIRIPIYIIVIASFVTMAALFMQAYLPELNAALGIY
                     VPLIVVNCIIMGRAEAFAGRNTPFKSVLDAIGMGVGFTLALSLISVIREILGTGSLKV
                     FGLNLLSVPVEPAMIFILAPGALLVMGLLLGLFAHMRNKKYEVKNE"
     gene            6004..6585
                     /locus_tag="VFF28_05580"
     CDS             6004..6585
                     /locus_tag="VFF28_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HZX45125.1"
                     /translation="MSDSLIAILIGAIFVNNFILTRFLGLCPFFGVSKKTKAAVSMGI
                     ALTFVTAGAAAVTWVINALVLIPLGLQYLQIIVFILIIATLVQFVEFFIRKSNPILYR
                     ALGIYLPLITTNCIVLGVSLINVQRNYNFLQSLTLGVAGGLGFMLAILLMSGIRERLE
                     LSKVPKAFSGLPIAFIIAALMSMAFLAFSGMIR"
BASE COUNT         1899 a         1314 c         1529 g         1941 t
ORIGIN      
        1 ttggtaaagt gtcattgggc ctcacctctt ttttgaggct catcatttta ccaacagata
       61 atcacagaat tagccatcct ataagtgtta cgcattcatc cccaaccaca agtggtgggg
      121 atttctgcta agaaatttaa aaagatatgg aaaggtaaga ataatggttg aacagatgta
      181 tcttgcggat ttccaaaaat acctgtccgg ttcaggattg aacctcaaaa tggatgtcct
      241 taaagatgga ccagggacga ttggaataag ttgccttacg ctagaacaaa gggcaggtga
      301 tcctatccta agccttgagc caaggcaaaa ccataaaggc taccaacttg aagtcataat
      361 atatacagac ggttcaggca ataacttttt gccaatagct gggggggagg ggagggctaa
      421 gatggaatac ggaaacgacc atagcagaag cgtgcatgct ttattagaaa gagaggaaat
      481 acccgaccta accttgactc cccttggatc cggagcttct gcaggtaggc gagaagagca
      541 gagagacagg gtccataaaa gaatccagga aaatggctat cgattgtatt ccttttacac
      601 aacaaagctt aacggaaggg ctgcatttgt tgttcatatg cccttagatc cttatgatgc
      661 tgtgaagaaa gtcgcaggtt ggactaggga attagcttca ttgaaaaccg cttgatttga
      721 ttattttctt atctccagtt attatcaacc cttcgacgct cggcagcttc tctatcaatt
      781 ccatgccttt ttccttgccg agaacaaaca cagctgttga aagagcatca gcgtcaaatg
      841 cgctgtctgc aataatggtg actgatataa gttcttcagc agaataacct gtctttggat
      901 ttacgatatg atgatatttc tttgaatcat caaaaaacct ttcgtaatct cctgatgtgc
      961 ttattgcatt gccgttcagt tttaagatag agatatactc gattgcatct cttggatttt
     1021 gaagtgctat cgcccaatca tcatctcctt tatttccaac tgccattatg ctgctctttg
     1081 cgtttatcaa agcatgctca atcccatcct tccttaaaac ttcagacgct ttctcaactg
     1141 catagccttt tgctataccg ccaagtgtta tcttcgtgcc gggctgcagc tttatcttat
     1201 cccttgagaa ctcgatgttt ttgtagccaa ctttctttag ttcttcgttt acctcttctt
     1261 ttgtcggcgg ccttttttcc tctgaaaatg tcttggtata caaatcaagg ataggctgga
     1321 tagttatatc aaagcttcca tcagaaagat tggaataata gatggctttc tccatattat
     1381 aatataggtc atctgaaaag ctttctatct ctccttctgc attgagccta tagacttcgc
     1441 ttgtttttag agtatggcta agaaggcttt ctatcctacc catctcctta aaggcagact
     1501 caatacctgc ttgtgctctt tgagcatcag tatcataaac agtaatggta aaggtggtgt
     1561 tcatcacact ctttgactcc tcgtacttgt ctaatggcgc tgtgcatccc gccagcaata
     1621 ataatgcgag gatcaacttt ttcatgaata aaaaagtgag gcataaatta ataaaggttt
     1681 tggcgaaaag caagttttta tatatccaaa tgcttggcaa gaagatatgc ttgatggcgt
     1741 aaagaaccac agagtcgtaa aaggaataca tttctatagc tttgggcttc ttagaaggct
     1801 atacaactgg accctgtcct gggctgatac aaaatatggc acccttgcat tgttcatact
     1861 tgccttcatg gagtcatcct tctttcctat ccctccagac atcctgctta tagcactgtg
     1921 catatccatc cataaaaagt catttaagta tgcagctatt tgcactgtag gaagcattat
     1981 cggcggtatg tttggatata ttatagggta tggtttatac gagtcggttg gggcatggat
     2041 aatagaagca cttcactatc agccgtattt tgatgctgta ggcaagatgt atgcaggtaa
     2101 tgcctttgtg gcaattatcg ctgctgcagt cacccctatc ccttataaag tatttaccat
     2161 agctgcaggg gtatggaaga taaatttctg gacacttgtg tttgcatcga tagtgggaag
     2221 agggttcagg ttctttttga tcgcatcact tatctacttc tttggcgcaa ggataaagac
     2281 attcatagat aaatatttca acctgatggt caccttattc ttaggtcttc ttgtcggcgg
     2341 ttttctggtg ataaaatacc ttgtttaggc cagtaattat gcggttgctg ccagtttcaa
     2401 taaatttata aatacctgta aaaatcaaca aggcatgatt gggggaatac atcctgagta
     2461 caacaagcta acggctgaca gcaagatagt tactgcgcct gcaccaaaga aggttgtttt
     2521 acccttgcag cagcatatag gcgctccttg cgagccacgt gtaaatgttg gcgacatagt
     2581 tcggtccgga caaaagatag ctgaaggcaa gggatttgtc tctgcaccta tacattcttc
     2641 tataacaggt aaagttactg cgataggctc tgcaaggcat cctgttcttg gcgagtttaa
     2701 tgctataact atagaagtag tgccgggtct cgaattgact gagcctgaaa aaaaattacg
     2761 aaaccctgag gacctcaaca aggatgagct aaaggcgatc ataaaagatg caggtatagt
     2821 tggccttggc ggcgctgctt ttccaactca tgtgaaacta agccctcctc ctgaaaagaa
     2881 gatagatact cttattatta atggagctga atgtgagcca taccttacct gcgaccatag
     2941 gataatgatc gaagagcctg aaaagatatt gaaagggcta aaagtgataa tgaagatact
     3001 tgagcctata aatgtatata taggcattga agataataaa gacgatgctc ttatcgtcat
     3061 gcagctaaaa ttaaaagagc ttgggcttgg tggcaaggtc aagatcgcaa agatgcaaac
     3121 agtatatcct cagggttctg aaaaaaatct gatacaatca atcactaaaa gagaggtccc
     3181 aaacgcaggc ggtcttccga tggatgtcgg cgttgttgtc caaaacgtgc agacagtcaa
     3241 agcaatatat gacgctgtct atgaaggtaa gccattgatc gaaagggttg tgaccatcac
     3301 cggcgctgta aaagagccaa agaacatact agtaaagata gggacaaacg caaaagagct
     3361 tatcgatttc tgcggcggct atgatggtgc tgttggaaaa gtgatatccg gcgggccgat
     3421 gatgggaata gcgcagcctg atgacaatat gcctgtgatc aagggttcat caggtgttct
     3481 tgtgcagctt gaggataaga caagcgttgc tgaaagggac tgcataagat gcggaagatg
     3541 cgttgatgtc tgcccggtaa agctgatgcc tactatgatc tatcagtata ctagaaaaga
     3601 ggattatgat ggtgcaaatg attactttgc catggactgc tttgaatgcg gaagctgcag
     3661 ctttatctgc ccaagcaaga ttccattgac gcattgggta aaggttgcta agagcgagct
     3721 tatgaagagg aagaagaaat gacgcttact gtttcatcaa atcctcatct cagggataag
     3781 gactatgtaa ataagataat gtggactgta atacttgctt tgttgcctgc ttctgctgca
     3841 ggagtttact tttttgggat gcgagcgctc actattataa tagtatctat ggcttttgct
     3901 atggcaactg aggctattat ccagtggctt acaaagaagc agataactat tgctgacggc
     3961 tctgctgcgc ttactgggct attacttgca cttgttatct cgccgcttgt tccatggtgg
     4021 gttgctgcta tcggagcggt atttgcaatc gctatcggaa agatggtatt tggagggctt
     4081 ggaaataata tatttaatcc tgcgcttgtc ggaagagctt ttttggtcgc ttcgtttcct
     4141 gctatcatga caagttggct tatccctgat ggaatcacta gtgcaacgcc tcttggactg
     4201 ctgaaagagc agggcatcaa aacaggctat cttcagctca tcgtcggaaa tatcggcgga
     4261 tcgataggcg agacatctgc tatcgcactt ttgcttggtg ctgcatacct attttataaa
     4321 aaagttatcg attggaggat ccctgctgca tatatcggag caacttttgt ttttacattg
     4381 gtctttgggc aggatccaat tttccatgtt tttgcaggag ggcttttgat tggagccttt
     4441 ttcatggcaa ctgattatgt aacttcgccc ctgactgcaa gaggaaaatt agtgtttggc
     4501 cttggatgcg ggctactcac cggcttacta agactttttt caagctatcc agaaggggtt
     4561 atgtttgcga ttttattgat gaacgctgct gttccattga tagacagata tactaaactt
     4621 aagccgcttg gctatagaaa ggtgataaaa tccgagggcc ctaccaaatg aaagatagtg
     4681 atttggccag ctcggctttt agcacctcaa agagccttca tggctctgtt gaggttaaat
     4741 catgaaccat atacttaaac tgggattgac attgatgtta gtcgcagtca tagctggcgc
     4801 tgcactttct cttaccaata ggtttacatt ggtgcagata gagaatcaaa aacaaaacgc
     4861 tatcaaagaa tcattgaaca tagtgataca agcagaatcc tttgaggaaa aggaaggcta
     4921 ttatgaagca cttgacaagg ataaaaaagt catcggcaaa gtccttaaag taaacactcc
     4981 agggtattca tccacgatac aggcacttgt tggaatcgac cttgagaaca agataaccgg
     5041 aatagatata gtcagccagc aggagacgcc tggccttggg gcaaaaatcg aaggagaagg
     5101 ttttttgagc caattcaaag gtaaaacttc taccgatatc aagctaagaa aggacagcgg
     5161 aaagatagat gctgtgactg gagctacgat atcttcaagg gcgatatcaa atggtgttaa
     5221 ggcaagtatg gagcaatacg gatttgctgc ccagacagat gatatcactg gcgcaagcaa
     5281 gcctgcaaat aatactgcag caaaaaatat gacagcgata cccaatacga taacaagagt
     5341 aaataattcg gctgccaaga aagctatgcc gtcttcaata ggtgcaaaca atgccacaag
     5401 gtaattggtg ggagcttgta aagggatttt ttaaggagaa tcccacgttt gcgatagtcc
     5461 ttgggctatg cccaactctt gcggtatcta cttcgcttga taatgcaata gggatgggag
     5521 gagctgccac atttgtacta atcggctcaa acctgataat atctgcgata agaaagaagg
     5581 tgcctggcga gataaggata ccgatatata tcatcgttat agcgtcgttt gtcaccatgg
     5641 ctgcactatt catgcaggca tatcttcctg agttaaatgc agctctgggg atatatgtcc
     5701 ctcttatcgt agtcaactgc ataataatgg gaagggctga agcttttgca ggcaggaaca
     5761 ctccattcaa atcagtgctt gatgcgatag ggatgggtgt aggattcacg cttgctttgt
     5821 cattgatatc tgtgataaga gagatactgg gcacaggctc cctaaaagta tttgggctaa
     5881 acttgctcag tgtgcctgta gagcctgcga tgatatttat ccttgctcct ggagctctcc
     5941 ttgtgatggg actattactt ggactatttg cccatatgag aaacaagaaa tatgaggtaa
     6001 aaaatgagtg acagccttat tgcaatcctg ataggtgcga tatttgtaaa taattttata
     6061 ctcacaaggt tccttgggct ttgcccattc tttggagtct cgaagaagac aaaagctgct
     6121 gtcagcatgg gtatagccct gacgtttgtc acagctggcg ctgctgccgt cacctgggta
     6181 atcaatgcac ttgtgctaat accacttggg ctgcaatatc ttcagataat cgtgtttatc
     6241 ctgataattg caacgcttgt acagtttgtc gagtttttta tcagaaaatc aaatccaata
     6301 ttgtataggg cgcttggcat atatctgccg ttgataacaa caaattgcat agtgcttgga
     6361 gtttcgctca tcaacgtgca gaggaattac aactttttgc agtcgcttac tcttggtgtc
     6421 gccggcggcc ttggctttat gcttgctata ctgctcatgt caggcataag agaaaggctt
     6481 gagctttcaa aggtgcccaa ggcattttcg ggattgccga tagcattcat aatagctgca
     6541 cttatgtcga tggcgttctt agcgttcagc gggatgatac gatgataatt gaatcaatcg
     6601 ttgttttggg aatactgggc cttggctttg gtatttttct tgcctatttt tcaaaaaaat
     6661 ttgaggtaaa acaggatcca aga
//
LOCUS       DASRUK010000126         3763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_99704, whole genome shotgun sequence.
ACCESSION   DASRUK010000126 DASRUK010000000
VERSION     DASRUK010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99704"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(300..1136)
                     /locus_tag="VFF28_05585"
     CDS             complement(300..1136)
                     /locus_tag="VFF28_05585"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HZX45126.1"
                     /translation="MIIILIVLTGLIIASVTDLKTREVPDWLSYGLICAGIGINLLFS
                     IIYWDYRYILYSALGLGAFLAIALLMFYSGQWGGGDSKVLMGLGALIGLKFDSFPFLA
                     SLLINILIAGALYGIAWTFFLAFRKRQRFILEYRKTSSKTAKARVIAGCTLVMSAPFV
                     FYFSQSIFAWIFFSAAVMSLITFYLWMAIKAVEKVCMIKKVTPDKLTEGDWIVKDVKY
                     KGKYLCGPKDLGIEKKQIREVMKLYRAGKIKKVLIKEGIPFIPSFLLAYIMTLVFGSV
                     FF"
     gene            1190..1885
                     /gene="uppS"
                     /locus_tag="VFF28_05590"
     CDS             1190..1885
                     /gene="uppS"
                     /locus_tag="VFF28_05590"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HZX45127.1"
                     /translation="MADQITFRLPRHIAIVTKGKHVYAEKHSKPIQQVYDKSNLIILS
                     TIKEIVRLNIPIATFYLLSTRAKELPNFSDVTDSLADFFSSLCENNLVVNNKIKISFI
                     GKWYDFPGKLVNSIKRVIDKTKDYDSFFVNFCINYDGQQEIVDAVKLIAMQIRAGKLD
                     PDLITKETIKENTYSSYFMPPDLIIINGSKRTSDLLLWDSSNAKVYFTNKLWPDFDKL
                     ELMDAIREYQKGE"
     gene            2007..2507
                     /locus_tag="VFF28_05595"
     CDS             2007..2507
                     /locus_tag="VFF28_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZX45128.1"
                     /translation="MLKKIIDQAKTPDLEIGQKIGLSQQAIFKIRHKLERIGIIKGYM
                     PIIDLKKIGVEGLAVIGIRFTNYVWEKYSEEEIAERIQKIPEVITSYRIPESAISHLL
                     VIGFKNMESKDRYLMKLQSKYAKEIQIVHVYPFSVDRIIKENHIGLLNMILDKKESVS
                     DEFFLD"
     gene            2566..2697
                     /locus_tag="VFF28_05600"
     CDS             2566..2697
                     /locus_tag="VFF28_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45129.1"
                     /translation="MRDLTVVRRNVCKHCKRQINGKAKLLGLALICPYCGKPSEEMM"
     gene            complement(3436..3573)
                     /locus_tag="VFF28_05605"
     CDS             complement(3436..3573)
                     /locus_tag="VFF28_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45130.1"
                     /translation="MSKLGNNRVAVTALEVMANVHTESTDYVVREAKGEVLLEGTLPY
                     I"
BASE COUNT         1213 a          745 c          773 g         1032 t
ORIGIN      
        1 cggaggatgt gctactacgg tcggatataa tgatgtggag actgtggaca actatatcag
       61 gaatcaagca caacatcatg cgattgcatg agataccccg acctttaggt cgtgggagta
      121 ggtcataacg ataatttgaa cttcaatggt tatgtatacg ataggtcaac agctgctaat
      181 ggctctacat caagcaaatg ctcagccaga gattaggact caaaaataga aaattccgcg
      241 cagctatggt aggtcttgca gcatgtggaa agcaccatct gcttagtttc tatccagcgc
      301 tagaaaaaaa cgcttccaaa cacaagcgtc attatatatg ccaaaagaaa actcggtatg
      361 aatggtatcc cttcctttat cagcactttc tttatcttcc ctgccctata cagtttcatc
      421 acttctctta tctgcttttt ctcaataccc agatcctttg gcccgcaaag gtattttcct
      481 ttgtacttga catctttgac gatccaatct ccttcggtaa gcttgtcagg cgtcactttc
      541 tttatcatgc acaccttctc aacagccttt atagccatcc acaggtaaaa tgtgataagg
      601 ctcatcacag ccgcggaaaa gaatatccag gcaaagatag attggctgaa ataaaaaaca
      661 aaaggcgcag acatgacaag cgtgcatccg gcaatcaccc ttgcctttgc tgtcttcgaa
      721 gaagttttcc tgtattcaag gataaatctc tgccgctttc taaacgccaa aaaaaaggtc
      781 catgctatcc catacaaagc tcctgcgata agtatgttga taagtaggct tgcaagaaaa
      841 ggaaagctgt caaattttag ccctatcaaa gctccaagcc ccatgagcac cttgctgtct
      901 ccgccacccc actggcctga ataaaacatg aggagagcga tagcaagaaa agcgccgagt
      961 cccagtgcgc tgtataatat atatctataa tcccaatata tgatagagaa caaaagattg
     1021 atgccgatac ctgcgcagat aaggccataa ctcagccaat caggcacttc tcttgtctta
     1081 aggtcagtga cagaagcgat tatcaggcct gtaagtacga tgagaattat tatcatcaat
     1141 ggtttaaaac agcaaatgtt tataaagttt ccttcactac cacggattga tggcagatca
     1201 gataaccttc agattgccaa ggcatatagc cattgtcact aaaggtaagc atgtatatgc
     1261 agaaaaacac agcaagccta tacaacaggt ctacgataag agcaacctta taattctttc
     1321 aacgataaaa gagatagtca ggctcaatat tccgatagca acattttacc ttctgtcaac
     1381 cagggcaaaa gagcttccga acttttcaga tgtcactgac tcccttgcag attttttcag
     1441 cagtctttgt gaaaataacc tggtggtcaa taataagata aagatctctt ttatcggcaa
     1501 atggtatgat ttcccaggaa agcttgtcaa ttcaataaag agagtgattg ataagacaaa
     1561 agattatgac agcttctttg tcaacttctg catcaactat gacggccagc aggagatagt
     1621 cgatgctgtc aagcttatcg cgatgcagat acgcgcagga aaactagacc ctgacctgat
     1681 aaccaaagag acaataaagg aaaatacata ttccagctac ttcatgcctc ctgacctgat
     1741 aataatcaac ggctctaaga gaacatcaga cttgctgctc tgggactcaa gcaacgcaaa
     1801 agtctatttc acaaacaagc tctggccaga cttcgataaa ttagagctga tggatgctat
     1861 aagggagtat caaaagggag aatgacttaa ttcttattta accattttct gagcatgctc
     1921 aacaagttgc ccaatagtat tataaaggca gtgagtttat tggttatatc gaaaaatttc
     1981 caaaactgac aaaaaatgag caaaaagtgc tcaagaagat aatcgaccag gctaaaactc
     2041 ctgatttgga gataggccaa aagataggcc tttcccagca ggcgatcttt aagataaggc
     2101 acaagcttga gcgcattggg ataataaaag gctacatgcc cataatcgat ttgaagaaga
     2161 taggggttga aggattggca gttattggta taaggttcac aaactatgtc tgggaaaaat
     2221 actcagaaga ggagattgca gagaggatac agaaaattcc tgaggtgatc acctcttacc
     2281 gtatccctga atcagcgata tcccatctgc tagttattgg attcaagaat atggaaagca
     2341 aggacaggta tcttatgaaa ctgcagtcaa aatatgcaaa agagatccag atagtgcatg
     2401 tctatccctt ttcagttgat aggataatta aggagaacca tattggcttg ttgaatatga
     2461 tacttgacaa aaaggaatcg gtctcagatg agtttttcct ggattaataa ccaccttctg
     2521 atgttttttg gttgattttc actaccatag gttgttatac ttctgatgcg tgatttaact
     2581 gtggttagaa gaaacgtttg taagcattgc aagaggcaaa tcaacggcaa ggcaaaactt
     2641 ttagggcttg cacttatctg cccttactgc ggcaaaccaa gcgaagagat gatgtgaaaa
     2701 aatgaaggtt tgtgaaaatc ccagagaatt tagaagtagg ttggataata atatcatctt
     2761 gatgataagt gattgcttaa gacttgggtt cataccccgc agctcggcta gcggttggga
     2821 ttttttattt atcattaagc tttgaagaga tatttttctc ttcacaggag ggaatcgata
     2881 tgtttgaatt tcaaggaaag caattgatac aaaataagga cggcgaatat cttgagttag
     2941 agaagcatag aaaggagagt gaggaagacg gatagtctac tgtttagaaa tacaggtaag
     3001 cctagaagca ggacaagtga ttttgaagat atgtttgagc cgagcaatat agtgaacaac
     3061 agcaaccgtg atcagaggga taaccgatga aaaacacttg tttacaatgc aacttggata
     3121 tagctaatga ccacatatac tgcgacagat gcatccagga aatagtagga aacgagaaag
     3181 gtattgagct tttggttaag taatggctat tttcttatag tggtgaaggt ttgggaagag
     3241 ctaagacagt ttgtcatcta tagtggattc aagcagttct attacctccc tcgtatttaa
     3301 tatatcgacc ttatcttgct tttttgacag ggactttatc ttggataaag cctgccctta
     3361 gaggataaaa tacagtacat gattccatac cgacaacact ggaatgatag tatggtgcaa
     3421 gcttctcctt gaggtctaga tatatggaag cgtgccttcc agcaagactt ctccttttgc
     3481 ctccctaact acataatctg tgctttctgt gtgcacgttt gccatcacct caagcgctgt
     3541 gacagcaaca cgattatttc ccaactttga cacttcttca ctattttgag tggaaaatag
     3601 agtttgacag ttgcgattaa acgtcaaaac tggttgtttt cacctgaaat caaggcgttt
     3661 ttagcttctt tttgatccta ctacctgact ttcggagcta aaactgaaaa aagcagtcaa
     3721 actgtcaaat tcatctggct aataaacgct aaaaaacgcc ttc
//
LOCUS       DASRUK010000127         9778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1926121, whole genome shotgun sequence.
ACCESSION   DASRUK010000127 DASRUK010000000
VERSION     DASRUK010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 9778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9778
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1926121"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1867)
                     /locus_tag="VFF28_05610"
     CDS             complement(<1..1867)
                     /locus_tag="VFF28_05610"
                     /inference="COORDINATES: protein motif:HMM:NF026240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZX45131.1"
                     /translation="MSKYYGQSLPYEEKILVFEKGLMKRVPIGEIVESNRDNISVVCF
                     DKGGKASIKKVSSLIKHRLNSRILEVSTASGRKIRVTDDHSLFTLSEKGISDIKTSQL
                     KAGESYIAVPNIIPFNPNPLYELDIFDMLKDNDFGVVVRGVSDELSSAVGKIGIKKAA
                     EILGVKPKYVYDLISKNIGIRVSKYSRLMEYCSIDVNKDFLQLSTKGKSMPCKLRLTE
                     DLMLFFGLWMAEGSYTNKNEVRLSIHRREEKFASQLCQKLFGMATIYRKKNSLGSDIY
                     LCSGILGKVMKNALGFNSGARIKKVPDFIYNLDKQSLSAFLRGYFSGDGSLNLTTPAP
                     MIEVSTESPELADDISYLLLQFGIVTKIYPRKARQQKRLCFADFENLEKFMDIGFIDS
                     EKNKAIASYLNLRKFSRRDQVPLTPAIKEFINCDKHFSAWKTSKTIGRNVLQRLAQPE
                     LTAALNGDIKWDKVVLVKEIDKSTDYVYDISVPGAENFISGFGGIYAHNSEENLRKKF
                     EEASKNAPSILFIDEIDAIASKREETRGEVERRVVAQLLANMDGLQSRGKVVVIAATN
                     VPNILDPALRRPGRFDREIEIGVPSKEGRLDILKIHTRNMPLYNKEQAIKDSTEIE"
     gene            complement(1904..2722)
                     /locus_tag="VFF28_05615"
                     /pseudo
     CDS             complement(1904..2722)
                     /locus_tag="VFF28_05615"
                     /inference="COORDINATES: protein motif:HMM:NF014419.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(2769..3185)
                     /locus_tag="VFF28_05620"
     CDS             complement(2769..3185)
                     /locus_tag="VFF28_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45132.1"
                     /translation="MTILGFNFRDIKVNRKEGIKGKINIKNNVVITDIKEHDLSLGEK
                     SQKALKFLFEFSSKYEPDFGEIVLGGDLVFIESASKIKEVLDGWKKSKKISKDIMAGV
                     LNTVLVKCNIEALILSQKVSLPPPIPLPEVKQEEAK"
     gene            3309..3728
                     /locus_tag="VFF28_05625"
     CDS             3309..3728
                     /locus_tag="VFF28_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45133.1"
                     /translation="MAIQREDRDIWNAYCEEDKVHYLAIDDLFKQAPKYKDSPRTFLT
                     ELLQLVQGRSQMIYGKLQEMFTTPGDAADKFWFYDRFMNVEETMQRRLNINLECAKDQ
                     IQGLAKYVSQISYFSTSEEVVGNILSQNKIRFDQSKQ"
     gene            3784..4449
                     /locus_tag="VFF28_05630"
     CDS             3784..4449
                     /locus_tag="VFF28_05630"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HZX45134.1"
                     /translation="MPKYKLVCFDVDGTLIDNIIFSWQLFHDYFRTDKKVREDVRQRY
                     MDGQISYLDWANHDIGMWLKKKAKKEDFFKALKANDVKLMQGALETIKELKKHGMKLA
                     IISGSLNIILEYAFPDYGKYFEDVFLSWIYFDAKGDVTGVKVTEFDMMKKADALKLIA
                     KREKLKLEECVFIGDHHNDVMIAKEAGLSIAFDCKDDGLRNIADVVIDKKDLRETLRY
                     ILE"
     gene            complement(4417..5133)
                     /locus_tag="VFF28_05635"
     CDS             complement(4417..5133)
                     /locus_tag="VFF28_05635"
                     /EC_number="6.3.2.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014288493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphopantoate--beta-alanine ligase"
                     /protein_id="HZX45135.1"
                     /translation="MIPKSHPRYASLMTRDKIVSGVDRGITSTHGLIAHGRGEAFDYL
                     LGEKTNKFALESIDAAARLLLRARHPVISVNGNVAALCPKEIAQLSEAIPAQVEVNIF
                     HASKEREGRIKEWLKANGAREVLVPGKGIIPTIDHNRRYTNENGILIADVVFVPLEDG
                     DRCEALIKMGKKVITVDLNPLSRTARASTITIVDNIIRAMPLLIRRVKQLKGKKAKKV
                     RYNNKKVLKKSLKYIAKRFS"
     gene            complement(5130..5651)
                     /locus_tag="VFF28_05640"
     CDS             complement(5130..5651)
                     /locus_tag="VFF28_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45136.1"
                     /translation="MILVKVIFAPKEVLPQQALPEQDLSAPKESLPEQVSVKLGLEGD
                     FPNKKEGTVVFDMKIFMENIIQVNNKVPKYVIFAESQAIPGLVFRYNVFESVFEGGLP
                     LIKTSEVKFLDGGKHLVSYTFKEGGPQKIFFDGKEVASGEFDSSKPLVTGLVISNLEE
                     APTIPFEGNVEFK"
     gene            complement(5687..6514)
                     /locus_tag="VFF28_05645"
     CDS             complement(5687..6514)
                     /locus_tag="VFF28_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HZX45137.1"
                     /translation="MRSATAFAPGNISCIFRIIEHRDRKKRHSLGLGFTTDKGAYVTV
                     SLAKKTEISTNGRKVHFPTVETVIKDLTDNEVRVDIRFEVPFGAGFGMSGASSIGTAY
                     ALNKLLNLGKTKRELAIVAHSAEALNGTGLGDVGGQYNGGMCAKFFRGNPLKGTRLPV
                     KDATIYYKVFSPLETKSIINNNRWKSKINRAGDKSLKKLRLLKDKKIGNIIAVSKDFA
                     SDSGLLIDRRVIQLIQEIERKGGHASMIMLGISVFSDTPFKGAKKVRISQKGAHLVK"
     gene            complement(6552..7724)
                     /gene="coaBC"
                     /locus_tag="VFF28_05650"
     CDS             complement(6552..7724)
                     /gene="coaBC"
                     /locus_tag="VFF28_05650"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HZX45138.1"
                     /translation="MKTVVIGVTSSIACYKAAFLIKKLKGFNIEVILTHNALNLIDKK
                     EFEKVLGKKVHIDMFYPGWNYKDYIKRAEMEHISLADKADLFLICPATANFIGKVANG
                     IADDLLTTSVMATAAPVLICPAMNCKMWENRIVQGNVRKLAANGYYLVMPEKGKLACG
                     YEGEGRLASLDVIEKEVRHIIENKDKLKGKRIIVTAGGTSEEIDPVRVITNKSSGKMG
                     IYIAEEAAKMGASVTLIRGRTEVEPFGNMKDIKVRSVSEMEKAIRKELRDNDLIIHAA
                     AVSDFTVEKTKGKISSGKGIVLTLKPTAKLIDSFKKANKKIKVIGFKAEHDVDKATLL
                     SSAKKLLKSADLVVANDVSKGVFGSEENDVYVVGKDKVEHIIGSKREIARILLLAI"
     gene            7805..7999
                     /locus_tag="VFF28_05655"
     CDS             7805..7999
                     /locus_tag="VFF28_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45139.1"
                     /translation="MCVSQVPGWFEWLSILSGPAIISGIDVYIFYNYPKIKKVGIIGW
                     SVVLILIWLMANGPRKAVWC"
     gene            complement(8000..8734)
                     /gene="panB"
                     /locus_tag="VFF28_05660"
     CDS             complement(8000..8734)
                     /gene="panB"
                     /locus_tag="VFF28_05660"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HZX45140.1"
                     /translation="MHTSQIRAMKGKEAITVLTCYDYNTAQLLEEIGIDLILVGDSLG
                     NVVLGFDSTQEVTMQDMIRHTGAVRRGAVSSFIVSDMPYKSDIDKEAALKNAQLLVDN
                     GADAVKVEGKPEICKYLVENGVAVMGHIGYLPQTDGKPSVKKDFVNLLKQAEALQAAG
                     VFALVMELVDKDTARKLTGFLEIPTIGIGSGPNCDGQVLVINDILGTFEDFKPKFARQ
                     YVNVNDLIRKGVKAFKKEVKAGDFPE"
     gene            complement(8716..9705)
                     /locus_tag="VFF28_05665"
     CDS             complement(8716..9705)
                     /locus_tag="VFF28_05665"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013099824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HZX45141.1"
                     /translation="MTKQSTQGYVPEKNINIDHFPHIQGYDFNSKFDLGKFLKAYSTT
                     GFQASKLGEAIEIVKAMRREKAIIFLSYTSNMVSSGVREIIRYLVEHKLVDVLVSTAG
                     GIEEDLIKTLKPFVLGNFNADGRYLFEKGVNRIGNIFVTNDRYTYFEKKMNEILASAY
                     ERQVKLKRPLCTTELIELIGKSIEDKGSILHWAARNDIPVFCPAITDGATGDMIFFMK
                     QRQPDFIVDVSEDIKRIVNIALNAKSTGVVILGGGVAKHYTLNAQIFREGAEFAVYIN
                     TADEMDGSDSCASTDEAVTWGKIKPNAPQVKVFGDATIIFPLVIAGVLCTQAK"
BASE COUNT         2616 a         2225 c         1758 g         3179 t
ORIGIN      
        1 cttctatctc agttgagtct ttgatagcct gctctttgtt gtaaagcggc atatttctgg
       61 tatggatctt aaggatgtca agccttcctt cctttgaagg aactcctatc tctatctccc
      121 tgtcaaatct tccaggcctt cttagtgcag gatcaagtat gtttgggaca ttggttgcgg
      181 ctatgactac gacctttcct ctgctttgaa gcccgtccat atttgcaagc agctgggcga
      241 caacccttct ctcgacctct cccctggtct cttcccgttt ggaagcgatg gcgtctattt
      301 cgtcgataaa taggatcgat ggtgcattct ttgaggcctc ttcaaacttc ttgcggaggt
      361 tctcttcgct attatgggca tagatcccgc caaagcccga gataaaattc tcagcgccag
      421 gcacagatat atcataaacg taatcagtgc ttttgtcaat ttccttgact aaaacaactt
      481 tgtcccattt tatgtcgcca tttaacgcag cggtgagctc aggctgagcg agtctctgca
      541 ggacattcct gcctattgtt ttagaagttt tccatgcaga aaagtgctta tcacagttga
      601 taaactcctt aattgctgga gtcaaaggaa cctggtccct tctcgaaaat ttcctaaggt
      661 taagatagct tgcgattgcc ttattttttt cactatcgat aaatccaata tccataaact
      721 tttccaaatt ctcaaaatca gcaaaacaaa gccttttttg ttgccttgcc tttcttggat
      781 agatctttgt tacaattcca aattgcagca aaaggtaaga tatgtcatca gcaagctcag
      841 ggctttcagt tgatacctct atcataggag caggagttgt aaggttaaga gagccatcgc
      901 cggaaaaata tcctctcaaa aatgcgctta atgattgttt atccagatta tagataaaat
      961 ctggcacttt tttgattctt gcgcctgagt taaatccaag agcattcttc atgacttttc
     1021 ctaaaattcc ggagcacagg taaatatcag aacctagcga atttttcttc ctatatattg
     1081 ttgccatgcc aaatagcttt tggcataatt gcgaggcaaa tttctcttcc ctcctatgga
     1141 tagacagcct tacttcgttc ttatttgtat atgatccttc tgccatccac aggccgaaaa
     1201 atagcattag atcctctgtc agccttaatt tgcatggcat gctttttcct ttggttgata
     1261 actgtaaaaa atccttgtta acatcaatgc tgcaatattc catgagccgg gaatatttgc
     1321 tcaccctgat gcctatattt tttgagatca agtcataaac gtacttgggt tttaccccta
     1381 gaatctcagc tgcttttttt atgcctatct tgccaacagc cgaagatagt tcgtcagaaa
     1441 ctcctctaac cacaacacca aaatcattgt cttttagcat gtcaaaaatg tccagctcat
     1501 ataaggggtt aggattaaaa gggataatat taggcactgc gatataagat tcacctgcct
     1561 tgagctgaga agtcttgatg tcactgatgc ctttctcgct taaagtaaat aatgaatggt
     1621 catctgtaac tctaattttt ctcccgcttg ctgtgcttac ttctaagatt cttgaattta
     1681 acctatgttt gatcaggctt gaaacctttt tgatagaagc ctttccacct ttatcaaagc
     1741 agacaacgct gatattgtcc ctgtttgatt caactatctc accgataggc actctcttca
     1801 tcaggccttt ttcaaaaaca aggatctttt cttcataagg aagggactgc ccataatatt
     1861 tgctcattat ctcaggcccg tttattagta taaaatgact gtttgtttcg tttgcaacag
     1921 cttttgcgac aagggttttg cctgttcccg gcggcccatg taatagcact cctttggggg
     1981 gttcgattcc aagcctctgg aatatctccg gatgcttaag tggaagttcc accatctccc
     2041 ttatcttttt tatttcttct tgcagtccgc cgatatcctc ataggagacc tcaaggattt
     2101 tttcctctgt aacttcaata gcctcaggat tgaaatcaac tgatgtctgc tctccgatta
     2161 tcacagcctg ctttggattt gtgtctgcga cgacgaactt gacatctcca aatccaaatc
     2221 ctggaagcac ttcatcacca agcaggctaa aaacatcgtc gaagaatgga ttttcaagca
     2281 tcgcagtcct tcttcttctg gtgccgccaa gtgatacgat atctcctttg actacagccc
     2341 ttccaagaag tccctgtttg aatatgtctg gtggtgccct tatcattatc cctttcttgg
     2401 caggagcgat gataactttc tttgcctcct tgacatctgc ttttcttacc ttgacaagtt
     2461 cgcctatgcc ggtctttgcg ttccttctga caaggccatc cgtccttatg atatttaacc
     2521 ctatatctcc tggataagcg cggtcagcga tagctacggt ctttctttcg ccttctatct
     2581 ctacaatatc tcctactctc acacctatct gctgcatgaa attattatct attctgacta
     2641 tgcctttgtt gacatcgtct tggatagctt cagctacctt aagcttgata gtcatatcgt
     2701 tctttttttt gtccatttcc atctctacag gtatgaaagt cgatttttaa atgttatgga
     2761 aaaaagaatt attttgcttc ttcctgcttt acttcaggaa gaggtatagg gggtggaagg
     2821 ctcactttct ggcttagtat gagtgcctct atgttgcatt ttacaagcac agtgttaagc
     2881 acgcctgcca ttatgtcctt gcttatcttc tttgactttt tccagccatc aagcacttct
     2941 tttatcttgc ttgctgactc gatgaagaca agatcccctc caagcactat ctcgccaaaa
     3001 tcaggctcgt actttgaaga gaactcaaac aggaatttaa gcgctttctg gcttttctcg
     3061 ccgaggctta ggtcgtgctc tttgatgtct gtgatcacaa cattgttctt gatattgatc
     3121 tttcccttta tcccttcttt tctgtttacc tttatgtccc tgaagttaaa tccaagtatt
     3181 gtcatcgata tctacctcct agttttaact caagtattcc cagagattat ataaatgttt
     3241 cgggggttgt tttttgaggg attgttctag catataagtt tttaaatatc attctattct
     3301 cttaataaat ggcaatacaa agagaagata gggatatatg gaacgcttat tgtgaggagg
     3361 acaaggtaca ctatctagca atagatgatc ttttcaaaca ggccccgaaa tacaaagaca
     3421 gcccaaggac atttttaacc gagcttcttc agcttgtaca aggaagatca cagatgattt
     3481 atggcaaact tcaagagatg tttactacac ccggtgatgc tgctgataaa ttttggttct
     3541 atgacagatt tatgaacgtt gaggaaacga tgcaaagacg ccttaatata aacttggagt
     3601 gtgcgaaaga tcaaattcaa ggattggcta aatatgtttc acaaatcagt tatttttcaa
     3661 cgtcagagga agtggttggt aatatcttaa gtcagaacaa gataaggttt gaccagtcaa
     3721 aacaataaac gcattctaca aattattcaa aaccttatta aacaaaataa aactaacatg
     3781 tttatgccta aatacaaact ggtctgcttc gacgttgacg gcactttgat agacaacatc
     3841 atcttctcct ggcagctttt ccatgattat ttcaggacag ataaaaaagt aagagaggat
     3901 gtcaggcaga gatatatgga tggacagata agttatcttg actgggcaaa ccatgatata
     3961 ggcatgtggc taaaaaagaa agcgaagaag gaggatttct tcaaggcatt gaaagcaaac
     4021 gatgtaaagc ttatgcaagg agccctagag acgataaaag agctgaaaaa acatggcatg
     4081 aagcttgcga taatctcagg ctctctaaat atcatcctgg agtatgcttt tcctgactat
     4141 ggtaaatatt ttgaggatgt ctttctcagc tggatatatt ttgatgcaaa aggtgatgtg
     4201 acaggcgtaa aggtgacaga gtttgacatg atgaagaaag cagacgcatt gaagcttatt
     4261 gcaaaaagag agaagttaaa gcttgaagag tgcgtcttta tcggcgacca tcataacgat
     4321 gtgatgatag caaaagaagc aggcttatcc attgcatttg actgcaaaga cgatggacta
     4381 agaaatatcg ctgatgtagt tatagataaa aaagatctaa gagaaacgct tcgctatata
     4441 cttgagtgat ttttttaata ccttcttgtt gttatacctt actttttttg ctttctttcc
     4501 tttaagctgt tttactcgtc tgataagaag aggcattgct cggattatat tatccactat
     4561 agtgattgta gaagcccttg cagtgcgtga aagagggttg agatccacag tgatgacctt
     4621 cttgcccatc ttgataagcg cctcgcatct atcgccatct tcaagaggca caaacactac
     4681 atcagcgata aggatgccgt tttcgttcgt atacctgcga ttgtggtcta tcgtgggtat
     4741 tatccctttt ccaggcacga gtacttctct ggctccgttt gccttcaacc actcttttat
     4801 ccttccctcc ctttcttttg aggcatggaa gatattgacc tctacctgtg caggtattgc
     4861 ctcactcagt tgagctatct cttttgggca aagtgcagca acatttccgt tcacagagat
     4921 gacaggatgt cttgctctta gaagcagtct tgcagcagca tctattgatt ccaatgcaaa
     4981 cttgtttgtc ttctcgccca gaagataatc aaaagcctct cctctgccat gcgcgataag
     5041 gccatgggtg gaggtgattc ctctgtctac tccagagact atcttatctc tagtcataag
     5101 gcttgcatat cttggatggc ttttaggtat catttgaact cgacattgcc ttcaaaaggt
     5161 attgttggcg cttcctcaag gttgcttata actaggcccg tgacaagagg ctttgaagag
     5221 tcaaactctc cagaagcgac ctcttttccg tcaaaaaaga tcttttgggg gccgccttcc
     5281 ttgaaagtat agcttaccag atgttttcca ccgtccaaaa atttcacctc agaggtcttt
     5341 atgagtggca gccctccctc aaacactgat tcaaatacat tatacctgaa gacaagtcca
     5401 ggtatagctt gcgattcagc aaatattaca tacttgggca ctttgttatt cacttggatt
     5461 atgttttcca taaaaatctt catgtcaaat acgacagtgc cttctttctt gttaggaaag
     5521 tctccttcaa ggccaagctt gactgaaacc tgctctggca aggattcttt tggtgctgac
     5581 aaatcttgtt caggcaaagc ttgttgaggc aaaacttcct ttggcgcaaa tattactttg
     5641 acaaggatca ctagaactac gaaagcaaag actatctttg caccatttat tttactaaat
     5701 gagctccttt ttgcgatatt ctcacctttt ttgctccttt aaatggagtg tctgaaaaaa
     5761 cagatatgcc aagcattatc atcgaggcat gcccgccctt cctttctatt tcttgtatca
     5821 actgtataac ccttctgtct ataagcaatc ctgaatctga tgcaaagtct tttgatactg
     5881 caattatgtt tcctattttt ttatccttta ataaccttag cttctttagt gatttatccc
     5941 ctgctctgtt aatctttgat ttccatctat tattgttgat tatagacttt gtctcaagag
     6001 gcgaaaacac cttgtaatag atagttgcat ccttcacagg caaccttgtt cctttcaaag
     6061 gatttcccct aaagaatttt gcgcacatcc ctccgttgta ttgcccgccc acatctccaa
     6121 gcccggttcc gttcaaggcc tcggcagagt gcgcaacaat cgcaagctct ctttttgtct
     6181 taccaagatt aagcagtttg ttcagcgcat aagcagtccc tattgaagaa gctccactca
     6241 tgccaaatcc agctccaaac ggcacttcaa accttatatc caccctgacc tcattatcag
     6301 tcaagtcctt gataacagtc tcaacagtcg gaaagtgcac cttccttcca tttgtagata
     6361 tctctgtttt ttttgccaaa cttacagtaa cataagcgcc tttgtccgtg gtaaatccaa
     6421 ggccaaggct gtgccttttt tttctatccc tatgttcaat tatcctaaag atgcagctta
     6481 tattgcctgg tgcaaatgct gttgctgatc tcataagttc ttatttaaga aaaaactata
     6541 aaaatgtttg cctagatggc taatagcaga attcgcgcaa tctctctttt gcttccgatg
     6601 atatgctcaa ccttatcctt gccaacaaca taaacatcat tctcttcgct tccaaaaact
     6661 cctttggaga catcgtttgc aaccaccaga tcagcgcttt tcaacagctt ctttgcactg
     6721 gataataatg tcgccttgtc aacatcatgc tcagccttaa accctataac ttttatcttc
     6781 ttatttgcct tcttgaaact gtctatcaac tttgccgtag gcttaagtgt aagcacaata
     6841 cctttccctg agcttatctt tccctttgtc ttttcaacag taaaatcgct cacagcagca
     6901 gcatggatta taagatcatt atctctcagt tcctttctta tcgccttttc catctcagaa
     6961 acacttctaa ccttaatatc cttcatgttc ccaaatggct caacctcagt ccttccccta
     7021 atcaaagtga cagaagcacc catctttgca gcttcctctg caatatagat tcccatcttt
     7081 cctgagcttt tgttcgtgat aacccttaca ggatctatct cttcagaggt tccgcctgca
     7141 gtaactatta ttctcttccc tttcaattta tctttatttt caattatatg cctgacctct
     7201 ttttcaatga catctaatga agcaagcctt ccctcacctt catatccgca agctagcttt
     7261 cctttttcgg gcatgaccaa ataatagcca ttggcagcaa gctttctgac attgccttgt
     7321 acgatcctat tctcccacat cttgcagttc attgcaggac agatcaaaac aggagcagca
     7381 gtcgccatta cagaggttgt caaaagatca tctgcaatac cgttagccac ttttccgata
     7441 aagttagcag tagcaggaca tatcaaaaac agatcagcct tgtcagcaag cgagatatgc
     7501 tccatctctg ccctcttgat atagtcttta taattccacc cgggataaaa catatcaata
     7561 tgcacttttt tgccgagaac tttctcaaat tcttttttat cgattagatt aagagcgtta
     7621 tgtgtaagaa ttacttcaat attaaagccc ttaagtttct tgataaggaa agcagcctta
     7681 tagcaggcga tgcttgatgt gactccgatc actactgtct tcatattata gatagaaaca
     7741 atatttgctt ataaaactaa ctcttgtcga acaatttata tagctgtcaa aaaagatgta
     7801 ggttatgtgt gttagccaag ttccaggttg gtttgaatgg ctttcaatat tgtcaggtcc
     7861 tgccataatt agtggtatag atgtctatat attctataat tatcccaaaa taaaaaaggt
     7921 aggtattatc ggttggagtg tagtacttat cctcatatgg ctgatggcaa atgggccaag
     7981 aaaagctgta tggtgttgat cactcaggaa aatctcctgc tttaacttcc ttcttaaaag
     8041 ccttgacgcc ttttcttatc agatcgttga cattcacata ttgccttgca aactttggct
     8101 tgaaatcttc aaaagtccca agtatatcat tgattacaag cacctgtccg tcgcaattag
     8161 gcccagatcc tatcccgatt gtaggtatct ctaaaaatcc agtcagtttc cttgcagtat
     8221 ctttgtccac tagctccatc actagtgcaa acacaccggc agcctgcaaa gcctctgcct
     8281 gcttgaggag attcacaaaa tcttttttga cagaaggctt cccatcagtc tgaggtaaat
     8341 atccgatatg tcccatcaca gcaactccgt tctcaacaag atacttgcat atctcgggtt
     8401 ttccctcgac tttgacagca tctgctccgt tatccactag taattgtgca ttcttaagag
     8461 cagcctcttt gtcaatatca gacttgtaag gcatatcaga gacgataaag ctgctcacag
     8521 ctcctcttct aacagcgcca gtatgcctta tcatgtcctg catggtaact tcctgggttg
     8581 aatcaaatcc aagcacaaca ttgccaagcg aatcaccgac gaggattaaa tcgattccta
     8641 tctcttcaag cagctgtgca gtgttataat cataacatgt aaggacagtt atagcctctt
     8701 ttcccttcat cgctcttatt tggcttgtgt gcatagaacc cctgcaatta ccagcggaaa
     8761 tattattgtg gcatcgccaa ataccttgac ctgaggagcg tttggcttta tctttcccca
     8821 tgtgacagct tcatctgtag aagcgcatga atcactgcca tccatctcgt ctgcggtgtt
     8881 gatataaact gcaaattcag ctccctctct aaatatctgg gcgtttaaag tgtaatgttt
     8941 tgcaacaccg ccgccaagga ttactacccc tgtgcttttt gcattgaggg caatgttgac
     9001 gattcttttg atatcttcac ttacatcaac gataaagtca ggctgtctct gcttcatgaa
     9061 aaatatcatg tcgcctgtcg ccccatcggt aattgcaggg caaaatacag gtatgtcatt
     9121 ccttgcagcc caatgcagta tcgatccctt atcctcgata gacttcccga taagttcgat
     9181 aagttcagta gtgcaaagcg gcctttttag ttttacttgc ctctcataag cgcttgcaag
     9241 tatctcgttc atctttttct caaaataggt atatctgtca tttgtcacaa atatattgcc
     9301 tatccgattc acaccctttt caaaaagata tcttccatca gcattgaaat tgccaaggac
     9361 aaaaggtttt agtgttttga tcagatcttc ttctatcccg ccggctgtgc tgacaaggac
     9421 atctacaagc ttatgctcga ctagatatct tattatctcg cgcacgccag agcttaccat
     9481 atttgaagtg tatgaaagaa agattattgc tttttcccgc ctcattgcct tgactatctc
     9541 tatagcctca ccaagctttg aagcctgaaa ccctgtagtg gaatatgcct tcaaaaactt
     9601 gccaagatca aactttgaat taaaatcata accttggata tgcggaaaat ggtctatgtt
     9661 tatgttcttt tcaggcacat atccttgggt tgattgtttt gtcattatcg tctttagaat
     9721 aagaccccta tttataaaga tatgccttac ttgtaatcgt ttagctttat cccactaa
//
LOCUS       DASRUK010000128        23772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1285889, whole genome shotgun sequence.
ACCESSION   DASRUK010000128 DASRUK010000000
VERSION     DASRUK010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 23772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23772
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285889"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VFF28_05670"
     CDS             <1..364
                     /locus_tag="VFF28_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45142.1"
                     /translation="RAEGIFPKEKKVELDEEVTWINKDLKEHKLACYLAGNRVAASSN
                     LKQDDSFSFTFQKEGAYTCIDAIYGLRSAIEVGEEGYTPTGGAVISDENAGSGLPLAS
                     IAIVSIIVLLFFVYGRKR"
     gene            348..980
                     /locus_tag="VFF28_05675"
     CDS             348..980
                     /locus_tag="VFF28_05675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZX45143.1"
                     /translation="MEEKDSVSLCMIVKDEERLLGSLLSRIKVDEIVIVDTGSVDDTL
                     RIAKKFTDNVHSYRWQDDFSKARNYSISKATKDWILILDADERLSKKDLIRLRSIIKN
                     SPKDILGYRFIQKTYHGRKVVSIRGICRLFRNKKSIRFVYPIHETVRESIKEKGGRIG
                     KTGIIIKHYSSLNQKKTDYYLRLLKQKLQTHPGSSAQKEIELENKLATLH"
     gene            complement(967..1617)
                     /locus_tag="VFF28_05680"
     CDS             complement(967..1617)
                     /locus_tag="VFF28_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45144.1"
                     /translation="MGEETPEVTITYETLFELLRREKDRSELQKLDSTFLANILRYLK
                     DKYAIINKQQNELFAAEERKKTEEQLENIRKLIKDLYNRREKKIAGVAIEKSRNKAMI
                     VDNSVFLKEEKEVFENLIKVLDMGRERILNYVLEMKEPASIEPIVIENKEEEQAKPVA
                     VRRDTKTVRFLSAVPKFVGRELEEYGPFFAEDIASLPAVIADILIKKGRAEEISEE"
     gene            1752..2528
                     /locus_tag="VFF28_05685"
     CDS             1752..2528
                     /locus_tag="VFF28_05685"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HZX45145.1"
                     /translation="MGGLFLKYRQVTPDFNLVKKVPTAEAASLESVLKAHERPDTEYR
                     PALTYFKDNSALGRIFGMGMSCAGTCQVIHPAGYVLSSYHQLEEYIRNPDQKAYFHDA
                     IIKDSYPIEILAYSKSSDLALFKIETSYEYPVVYIAEKSPKGSVFTSFFDNIGAVKNQ
                     AMNVLSGNTSVATSAELELSICAGRVVSHSADGKYFTIQETPDVSIERGNSGSPVWNL
                     DNELVGVSIEIDLQGNVIFAGTRNIRSLLETYVTACKRSP"
     gene            2560..3750
                     /locus_tag="VFF28_05690"
     CDS             2560..3750
                     /locus_tag="VFF28_05690"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZX45146.1"
                     /translation="MAILSFQDLSFEEKEDKVRVNFLRIFYFYLNKEDLNDIGTYQVK
                     KNEIEFPNTPQPKAEKKFYVLLEKGFKLLKNSLNQKPAMYIHKNSGIPLIGNVAFGLV
                     DRGTNVIEVKPVTGCNIKCVYCSVDEDKRPVDFVIEESYLAEEFKKLVKFKEANDIEA
                     HIASQGEPMMYAPLADLIRDISQLKEVKTISIDTNGSMLTEKKVDELIKAGLNRFNLS
                     INALDPNLASKIAGMPCNIGRIQDICRYIRKKTNLIITPVLIPGINDDEMPKIIEFAK
                     EIGADIGIQNFMVYRLGRNPVKPISFEGFYAKLKEWDAKYHMKLIKTEKDFNITKTKQ
                     LPKPFRKGEVIEAEIICKGRLPNEKLAVAKDRTISVSTSKEKGKVRLRIERTKHNIFI
                     GTPI"
     gene            3760..5175
                     /locus_tag="VFF28_05695"
     CDS             3760..5175
                     /locus_tag="VFF28_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45147.1"
                     /translation="MLTFSNRNKATLVFIFFLIIFSILQYRVPEIIGFDGWLHIKMAD
                     LIRHEGFINPAPYSTILVDTYADNQLGFRILLIPFTFLGLIHGAKIASILFASAAFTI
                     FYWYLRKNNSINYPFFWTAAYAFASYDLLYRFLEPRAMPLAILSLVLTLFFIEKRMYK
                     SIFILSLLFGWLYYGFVFQLFIISVFLAIDYLKKRKLDINLILYPIAGIALSIVINPY
                     FPASISHLYTQLFQVNLVSNLYNAEWKSWGILDFIKFNWIYMLLYLTSVGAIIRNQKF
                     DKKQLGLFIISTIFLVAMLKTRRMHEYFAPFSVLFAAFCLGNKENNSHARYISVAALV
                     LISLFSLVSLNEYIHNNHFLPWYKEGADFLRTLPEGSKVFINAYTYTYFFFYNPSLEY
                     THGIDLTYSYLKDPQKFERYISVLQGKDPGINIVKDYDANYAVIGKIRQDINLFDYMI
                     NYQDDFELIYEDKNLGILRVK"
     gene            complement(5232..7751)
                     /locus_tag="VFF28_05700"
     CDS             complement(5232..7751)
                     /locus_tag="VFF28_05700"
                     /inference="COORDINATES: protein motif:HMM:NF016980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45148.1"
                     /translation="MKLRKAIKKIVAIGATTLLGATMIASAADLSEYPAPFISGGKFS
                     GMLVVGDKAAAEDVIGVSDIAMSLQYAATSGTSTGTSTSVEGDAWLAGTSSKKLEMTN
                     QQGSTGNETIRDIQTSIDEDELTSLADGSITTEKGTAGYNQYINFDETAKNGGYVKFM
                     ENDDDVVGDFLYFKSGVQIARYSLEFKTSLKSDVEDSTGSKTSTGTYLGDLEDEKITI
                     LGKEYNIVKARRTNGNGNNPELTLMGGSVKDTLSEGETKTYLDGKYEVTVTAITDTGT
                     IYAKFNVNGETTKSLKDGDSATLSDGTELGIADIIPNEAGDVTSDLVEFYLGASKVYL
                     KDTAIQDTASSNALEVGSEKIDDAYVKITGSDDNATFSIDSLQINITADDDFFVAAGH
                     SLTEYMEEPEAFLGLWDIKYEGLDNVETETYKIRTSGSDTYELQFVDGDGNLATVPIA
                     YTSGTTVLARFGDNDNTLVLNGTQVIKKNDYLIVTDVSQDDGNRKTYALKYKGADKVA
                     SGETALVKFDNLGTGSRIEQSFTEAVGDTADATLKIGGSSFAVINATADTSDDFDVRV
                     DLDADGTVTADTDVVAINTNAGLKIVLTNETTSVIVTLSTPNSNHYDNIVPAIASFNI
                     TASSGEVSFVEHTTDEITYVAPSEETNTKYAYTSMGAKYKWESPTNDPAQLTVEYPKE
                     QRLPLVYITTAGAKVTKGTSASGVESVSLNKIEVGATKLASEITDVKAQNLILVGGPC
                     ANAAAAEAKSNPTDCAAGFEAGKGLIELVETGSGNVALIVAGYDAKDTRAATSILADY
                     GNYALKGAKMEVVTATSTVKEVTATAATTEAATTETPAVVE"
     gene            complement(7918..9636)
                     /locus_tag="VFF28_05705"
     CDS             complement(7918..9636)
                     /locus_tag="VFF28_05705"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZX45149.1"
                     /translation="MKARKLHVILIIALSALAYLNTFWNPFVFDDQFFIVDNTEIRSL
                     RNIPQFFAGPSTGNLYRPLRGVLYAFTYSIWELNPFGYHLNAVLLSIGISILAYLIAS
                     QLFKNKTYAFVAGLLFAIHPVHTERVANMTGGFDLLGVLFLFLAFYSYILFRKNNSDK
                     AFVFSITFFILGLFSSEEAIIFPILVIAYDLCFVWPKSWLKHYLPIVLVSGGYLSLRY
                     LVLGQIGRTDVFFMGDTLTRVLSTAVIFARYIFITLFPFWLSVDYNVTAYTSLGFTAA
                     ACLALLAFVCFACIKCYKSHRLAFFSICWFFIALIPFSNIFPVQTFMADRYLYVASLG
                     FVLLLSYLISRLNHRKAAFAVVLVLVALYLPATILRNAEWKSDYALLSSAVERQPKSS
                     IAHNDLGQVYQTLGDFGKAAEEYIIALRLNDRNHVAWLNLGTVYGEAGNYSMAEYYLN
                     KSLSIYRSYKGHNNLGLIYWKMGKIDKAILELGMAIELEPSLSKAYMDLGVIYANDGS
                     INLALAQFERAISLNPAVPDSHYNLAVLYEYLNKTDEAHKEYTFAYQLEPGNKLYKDK
                     AIGGYR"
     gene            9807..10760
                     /gene="corA"
                     /locus_tag="VFF28_05710"
     CDS             9807..10760
                     /gene="corA"
                     /locus_tag="VFF28_05710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HZX45150.1"
                     /translation="MITVLEYTKDKVVQGNLSNICQPKTFTWIDVFEPTPEEIKQISD
                     ISQTQLSDLNMVLGQDERPKVIEIEGPYSLIIFGAPAFEEGGVKTTPVYIYTSKLHNC
                     VITIRNQDTKSIHKLKEAIESRKGFFDKGSSYFVYRLIDEILNTFYLVLEDIENKIDQ
                     IEDSIVSAPDISLISKIFEIKKTLIYFNKTITANREVIASIEKEYLTEIDKKRAKQFR
                     TLYNDTVQLIDMSSTYRDILTGTLDTYLSSVSNNLNQVMKTLTVLTSFIMVPTLIASI
                     YGMNFQFMPETGWKYGYLFAVGLMGLSTLGTYLFFKRKNWI"
     gene            complement(10776..11627)
                     /locus_tag="VFF28_05715"
     CDS             complement(10776..11627)
                     /locus_tag="VFF28_05715"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZX45151.1"
                     /translation="MAIGTEFLAELSKFNLVISKRVTSKYTGPRKSNAIGRGLIFREH
                     RIYAPGDDIRDIDWRVFARTDDLYIKKYEEERNLTLHIVVDFSASMGYGKNISKFDYA
                     AMIGTGFAYLAMKENERFQFATFSEELSVFQPRRGMGQLAAMIDHLNAIKTHGKSRLK
                     DAIHQYKKLIGSRAMVIIISDFLMPIDEINEVLFMLGSNEVKIIQVLDPVEKELKMTG
                     DFKLKDSETGDKLVTHISPRLQMQYQKLMSEHAGKINDTCARLKMKFYTVTTDTPIFD
                     AFYQILS"
     gene            complement(11634..12572)
                     /locus_tag="VFF28_05720"
     CDS             complement(11634..12572)
                     /locus_tag="VFF28_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZX45152.1"
                     /translation="MVKNYKIVFDTIQKEVGRVIIGQEDLIDQIMTAMLCDANALLEG
                     YPGLAKTLTIRTLSEVLDMKFSRIQNTPDLMPSDITGTYIISEKGGRREFEFQPGPIF
                     SNIVLADEINRATPKSQSALLEAMQEKQVTVGNSTYKLDLPFFVLATQNPIEQEGTYP
                     LPEAQADRFLLKIKVGYPKPEEEMKIVDRFTNPLVENKLRKILNKQQLLHLQQTTREI
                     PIANDLKRRVIDLIDATRTKKDLVQYGASPRASIGLILASKARALIMGRNHVSSEDIE
                     ALAQPVLRHRIVLNFEAERKGLTVDDAILELLKKVK"
     gene            complement(12575..12715)
                     /locus_tag="VFF28_05725"
     CDS             complement(12575..12715)
                     /locus_tag="VFF28_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45153.1"
                     /translation="MVKNYKIVFDMSEIALGARSDALAQSKPAISEHAQKRFQCEVMR
                     RW"
     gene            complement(12725..13510)
                     /locus_tag="VFF28_05730"
     CDS             complement(12725..13510)
                     /locus_tag="VFF28_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45154.1"
                     /translation="MKDFRKVLKEVNLAIDEIMAFDIILTSVLIFLIFYFFLMMFNLN
                     PWYSLFPTLAYLAFLLYIDMNKNKYKIVEDKYAPLYEKLRTAADNVHLDNMVVEELEE
                     EVTKDLSNVNVSSFVKLSRVSYKVLGSVILCLLILFASIYNMHFGDLSLVLDKADEFI
                     RGSGGGVSDGKGNILAAGEGEGEDDIFGQQGMAALGNSETQVKIRQPSVEIIGTDENT
                     IPDKEFEETFPDEIFAVSSGAYEEKITVENQQLVKDYFKEISK"
     gene            complement(13525..13986)
                     /locus_tag="VFF28_05735"
     CDS             complement(13525..13986)
                     /locus_tag="VFF28_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45155.1"
                     /translation="MSIIKRDVNFVFFILIIATVVSFAGFTAYYQVSFRNMNDEYNTK
                     LAELNKVTKDLLEKKALLMQTSSELNTTKQGREELGQKYTELKDERDTLETERNNYKE
                     SLVVTRAELGQKTEQVTSLSSQLADMTTKYDRMKEDKDYYKNMLDICEDES"
     gene            complement(13992..16463)
                     /locus_tag="VFF28_05740"
     CDS             complement(13992..16463)
                     /locus_tag="VFF28_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZX45156.1"
                     /translation="MMLGLGKVCFSQYCLIDPFYLYLAVPLAIIMGIILWINFAGFKK
                     REEKKAFAKSHRLDRIIFLVLRTIAFVLLLIAIATPYTTQETVTEGNPSLTILADNST
                     SFDVFNTSIAGSLRDELSRYFPTNLKTSAYGERSAIGDGILQNAEGDDHLLVISDGNN
                     NYGRDIGDAILFSSVLNTTVHTLDLKPLKKDIAVFIDGPSQVIEQSETTFYARMNNLG
                     GVKYNKLEVFVDGNEIKLGSDGSFNWRFSKGYHKITARILFPEEDLFIQNNIYYKAVK
                     SLPRPKLLYVGERSGPLQSALERIYDVTAQTDIPGDLLTFDAVILNDLKIDKIGSDKV
                     DLLNAYLSEKGKGAIIVGGQNSFDNGGYKGSYFESMLPVDIGVGKREREEIYNIFFII
                     DLSGSVSLPFKSGSPETVLDVEKSLMTGLINGLNPDDKIGIIGHTTKRYCVPQEAQCI
                     LKPIIEMPNLYELISQLQIPYGPNGEPPGTAIGPALMRVRQSLYDLKGSKNAVILSDG
                     DYGESKTYLDDQIRTMGSLGIKVYTVGVGYNVKRDTLQHMAELGGGLYFEPDEAEKLK
                     IMFIGDEEEQRCQKATSGRVLVMDSSHWITKGTEGINGNIGGYNLVVPKSWGRKLLAT
                     DCGRTILTAGRYGLGRIAVLSTDDGSKWSGGFYSEQNSKVLMRAVNWAIGDFTKDMQL
                     DVRAKDTTLGKPTDINVISEAAPEYKGLEFAKIDTGLYSAKFMPNETGFYQILDATLA
                     VNYNDEYLKIGLNPELKDLVGYSGGEVFAPSEIERIKNTIISMSRRSKVETINYRWPF
                     IMAALIIFLLDILIRRIRQNINLMR"
     gene            complement(16460..18301)
                     /locus_tag="VFF28_05745"
     CDS             complement(16460..18301)
                     /locus_tag="VFF28_05745"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HZX45157.1"
                     /translation="MVFSNIAGLYALLAIVPFVIIYLIRPKTFERVIPSLMFIMKERN
                     KLIKASFLQRVLMNLLFFLQLAIIIMLAIAAASPYLEIPHSIMVRNSVIVLDDSASMQ
                     AKDGMETRFEQAVGKAKDNLGLKNTIILAQSTPAIVLEHGSSGNALNLLNRIVPKATT
                     TNIGDAMLMAGDMLGDKKGVITVISDFIQTEGSDILIAKRELASRGHSVNLIDVNSKA
                     ENIGITDIFVDKKETIIEIKNYKDSDEKIRVSLSKDGRKVDSRELSLLAKSKEKVLFE
                     TLGGLSELTLEVNDDFLVDNKAFVSSPEKKAFRILLIANNPDESKIKSALSVIGAELE
                     VREPPTVNAYNIDHDIVVIGEIKKDLFVPTDFTDLKKYVDKGGKLIISAQEDLGEINM
                     AGLLPINVKSKEDRQTSICADIIGNLFPKDPFSDEPCFNSASKYISAEAQNGTLVMAS
                     NKEDGSPAIVVKRQGNGEVIYYGIIDKYSGFYSDPFYPIFWNNLVNYLMKTENIKDFN
                     YKTGKIVSAASPEIKTPSSRISASRLVLDETGIYEVDGKKIAVNLGDEKESDINKETL
                     QLKSALGEISEEKVKDTSNLDLEVPLLIAGVLLLFLEFLYIKRRGDL"
     gene            complement(18291..18500)
                     /locus_tag="VFF28_05750"
     CDS             complement(18291..18500)
                     /locus_tag="VFF28_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45158.1"
                     /translation="MEPKFVGKAKLTKQGQVTLPNEARQDLNISPNADIYWYELGKNL
                     IAVTELVSPKDLEVVLRKKRGSNGV"
     gene            complement(18681..19523)
                     /locus_tag="VFF28_05755"
     CDS             complement(18681..19523)
                     /locus_tag="VFF28_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45159.1"
                     /translation="MHPGQGGVPVDEDLSTEVDRAAFALMKDEGPLPMVDFPESQRIV
                     ANKFLTGMAVDDLDLACLIQQVVAENRTFLMDPSNSPTDYREAANLSQPYLYLSGNGV
                     LNTCNFAQADVLSRLFSLNCGQIELYGLQNSDIMGRFFYIHKDSKHLFIIARLKGSDT
                     YVLIDTSIRQFFRKNHAVANSEEMGHLLMQGYHSLTDSMLVKYCGVFYGSEIPQNMVK
                     GGLTLPAFMNCESIMQYKEGPNKCYLVCERDNISNRYNKKYAPFNLFTDMRIAKHLSA
                     LNRD"
     gene            19632..19838
                     /locus_tag="VFF28_05760"
     CDS             19632..19838
                     /locus_tag="VFF28_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45160.1"
                     /translation="MAKLSRIEVILLMFLAILFGAVFLFAFLSVIYGYYLIQIGLPQI
                     VISIAILLFALAALLKVSWHIVSY"
     gene            19873..21768
                     /locus_tag="VFF28_05765"
     CDS             19873..21768
                     /locus_tag="VFF28_05765"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015848776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HZX45161.1"
                     /translation="MRILTLHSDFIEFEAKKKAIKEPEELKNKQERIEECLVVFISVE
                     NKDESDPASAAANLVKNIKEIAAMVNEKKVVLYPYVHLSSSPSSPATALKVLDLAESG
                     LKGEYEVHRSPFGWYKSFNISVKGHPLSELSREFGPEKEEAKPGEAKEAGKEEETESL
                     KKERKLTSYWHILSPDGTLSDVSKFNYQNHEKLKKFAFYEKEKTRAVDQEPPHAVLMR
                     KLELVDYEPASDGGNLRYYPKGRLIKKLIEEYVTDRVISYGGVEVETPIMYDMNHPTL
                     SKYLQRFPARQYQIESDKRKFFLRFAACFGQFLMAHDATISYKDLPLKLYELTRYSFR
                     REQSGELTGLRRLRAFTMPDVHAMVADMEMAMSEYKVRFNLCIDVLRKMGIEPKDMEM
                     ALRTTKKFFEENKEFMLGLVNHFGKPVLLELWDERIFYFVLKYELNFVDNLNKASALS
                     TDQIDIENGERYDINFVDKEGNKNHPLILHCSPSGAIERVIYALLEKAAFDQKQGKTP
                     KLPLWLSPTQVRVIPVTEEFQSYADEITDCLRSSSIRADIDDRILHVGKKIRSAEVEW
                     VPFIVVVGEKEKAAGNINVRIRETKEQKEMSVKDLIDKIKSVTEGMPFKPLALPKELS
                     KRPVFVG"
     gene            21894..23126
                     /locus_tag="VFF28_05770"
     CDS             21894..23126
                     /locus_tag="VFF28_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HZX45162.1"
                     /translation="MKKESFRWLVVIGALIIQLCLGAIYSWSVFVNPLKEAYGFTTTQ
                     TQIIFSVALATFAITMVYAGRCQDKVGPRKIAMIGGLLLGMGYMAARFAGSSFSGYLL
                     TIGVIGGAGLGFGYVCPIAACVKWFPDKRGLITGLAVAGFGAGAWLFAQIASNLMESI
                     GIPATFMTLGIIFLIAIFFSSLLLVNPPEAWVPKGFKVEKKKAKAAVNFTARQMLKTK
                     QFWFLWLMFTFAASAGLMVIGNLKPFGINSGLTAALAGSAVGILALFNGAGRITWGFL
                     SDKIGRTRAMTFMFILQGMMMLILIKMGATPLTLAIAAAWVGFNFGGNFALFPSATAD
                     FFGTKNLGINYGWVFTSYGVAGILGPILGGRIFDLTGSYLYAFMPSGILCILAAGLAI
                     ATKRPAAQAAASAKAAPD"
     gene            complement(23123..23596)
                     /locus_tag="VFF28_05775"
     CDS             complement(23123..23596)
                     /locus_tag="VFF28_05775"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HZX45163.1"
                     /translation="MQIAKKEDCQRCKGCCIFDRKETRFAPVFTLKEIAGLDAEFILV
                     SNNLYKPKLVFSEGNWQCPFLDANHLCSIQHRKPLCCNIWPFIFMKNVFGQLVIACFE
                     KKICPSLRKMSEGEFKGRVLEFYNYIKKEKIIEYVKDNPGLVWKTEPFAFVIKKI"
BASE COUNT         6665 a         5188 c         4796 g         7123 t
ORIGIN      
        1 cagggcagaa gggattttcc ccaaagaaaa gaaagtcgaa ttagatgaag aagtgacttg
       61 gatcaacaaa gacttaaaag agcacaagct ggcttgttat ttggcaggaa acagggttgc
      121 agcaagctca aacttaaagc aagatgattc attctcgttt actttccaaa aggaaggagc
      181 atatacatgc attgatgcta tatatggcct aagaagcgca attgaagttg gtgaagaagg
      241 atacactcca acaggtggcg cagtgataag cgatgaaaat gcaggttcag gacttccgct
      301 ggcaagcatt gcgatagttt ccataattgt cctgcttttc tttgtttatg gaagaaaaag
      361 atagtgtcag cctttgcatg attgtgaagg atgaagaaag acttctaggc tcattgctaa
      421 gcaggataaa agttgatgag atagtcattg tcgataccgg ctcagtggac gatacattaa
      481 gaatagcaaa aaaattcaca gataatgtcc atagttatag atggcaggat gatttttcta
      541 aggcaaggaa ttattccatc tcaaaggcca caaaggattg gatactcatc cttgatgcag
      601 acgaaagatt gtcaaaaaag gatctcataa ggttaagatc gataataaaa aacagcccta
      661 aagatatcct tggctaccgc tttatccaaa aaacatatca tggcagaaaa gttgtgtcga
      721 taagagggat ctgccgcttg ttcaggaaca aaaaaagcat acggttcgtc tatcctatac
      781 atgagacagt cagagaatcg ataaaagaaa aaggaggaag gataggaaag acaggaatta
      841 ttatcaagca ttattcctct ctaaaccaaa aaaagacaga ctactaccta aggctcctaa
      901 agcaaaaact gcaaacccat ccaggaagta gtgcccaaaa agagattgaa ttagaaaaca
      961 agctcgctac tcttcactga tctcttctgc ccttcctttc ttgataagta tgtccgcgat
     1021 aaccgcagga agcgatgcga tatcctctgc aaaaaacggc ccgtattctt caagctctct
     1081 tcccacaaat ttaggcacag cagatagaaa cctcactgtc ttcgtatctc ttctcacggc
     1141 gacaggtttg gcctgctctt cttccttatt ctcgataact ataggctcga tgctggctgg
     1201 ctctttcatc tcaagcacat agttaaggat acgctctctg cccatatcaa ggacttttat
     1261 aagattttca aagacttcct tttcttcttt caagaagaca gaattatcga cgatcatcgc
     1321 tttgttcctg ctcttttcta tggcgacgcc agcgatcttt ttttctcttc ggttgtataa
     1381 gtccttgatt agtttcctga tgttctcaag ctgttcttct gtctttttgc gctcctctgc
     1441 ggcaaagagc tcgttctgct gcttgtttat gatagcatac ttatccttta ggtatctaag
     1501 gatattggca agaaaagtgc tgtcaagttt ctggagttca ctcctgtcct tctctcttct
     1561 taatagctca aacagggtct cgtaagtgat tgtgacttct ggtgtttcct cccccatatg
     1621 aagttatgta taaggttgtt atttaaatat ttagcgcata aattcttaaa gccactttca
     1681 ctcctaggct acatggggca aaccagaaga aattttttgc atgttgctgc agcgacaagt
     1741 tctttggtaa catggggggc ttatttctta agtataggca agtcacacct gactttaatt
     1801 tagtaaaaaa ggttccaacc gcagaggcag cctcattgga atctgtcttg aaggcgcatg
     1861 agaggccaga tacagagtat cgtccagctc tcacttattt taaggacaat agtgccttgg
     1921 gaagaatatt tggtatgggc atgagttgtg ctggaacatg ccaagtgatt catcctgcag
     1981 gttatgttct gtcaagttat catcaattag aagagtatat tagaaatccc gatcaaaaag
     2041 cctattttca tgatgccatt ataaaggata gttaccccat agaaatactg gcttattcaa
     2101 agagctcgga tcttgccttg ttcaagatag aaacaagcta tgagtatcca gttgtttata
     2161 ttgcagagaa aagcccgaaa ggatcggttt ttacatcatt ctttgacaat attggtgcag
     2221 taaaaaatca ggcaatgaat gttctctctg gaaacacatc agttgcaact agcgcagagc
     2281 ttgaattaag catttgcgca ggacgagttg tcagtcattc tgcagatggc aaatatttta
     2341 caatccaaga aacaccagat gtctcaattg aaaggggtaa ttcaggttca ccagtctgga
     2401 atctagataa cgagttggtg ggcgtcagta ttgagataga tctccaagga aatgttattt
     2461 tcgcgggaac aaggaacatc aggagcttac ttgaaactta tgtaacggca tgcaaacgct
     2521 ctccataagc tatttaaaca atctactatc tatatcatta tggccatcct atcattccag
     2581 gatttatcct tcgaagaaaa agaagacaaa gttagggtca acttccttag aatattttat
     2641 ttttatctga ataaggaaga tctaaatgac attggcacct atcaagtaaa aaagaatgag
     2701 atagagtttc caaatacccc tcagccaaag gcagaaaaga aattttatgt cttgctcgaa
     2761 aagggcttca agcttctaaa aaacagcctt aatcaaaagc cggccatgta catccacaaa
     2821 aactcaggca tacctttaat aggaaacgtc gcctttggcc ttgtggatag aggtactaat
     2881 gtaatagagg taaagccagt cacaggatgc aacatcaaat gtgtatattg ttctgtcgat
     2941 gaagataaga ggcctgttga ttttgtgata gaagaaagct accttgcaga agagttcaag
     3001 aagttggtaa aattcaaaga agcaaatgat atcgaagccc atattgcctc ccaaggagaa
     3061 cctatgatgt atgcgccgtt agctgatctt atcagagata tatcccagct aaaagaagta
     3121 aagacaatat ctatagacac aaacggctcg atgctgacag aaaagaaggt tgatgaactc
     3181 ataaaggcag ggttaaaccg tttcaacctg tccataaatg cacttgaccc caatctagcc
     3241 tcaaagatcg caggcatgcc ttgtaatata ggaaggatac aggatatctg caggtatata
     3301 agaaaaaaga ctaacctcat aatcactcct gtccttatcc cagggataaa cgatgatgag
     3361 atgccaaaga taatagagtt tgcaaaagag ataggagcag acatcggcat ccaaaacttc
     3421 atggtatacc gattaggaag aaatccggta aaaccgatat cgttcgaagg attctatgcc
     3481 aagctaaaag aatgggatgc aaaataccac atgaagctga taaagacaga aaaggacttc
     3541 aacataacga agacaaagca gcttccaaaa ccatttagga aaggagaggt tatagaagca
     3601 gagatcatct gtaaaggcag gcttccaaat gaaaagcttg ctgtcgcaaa agacaggacc
     3661 atctcggtca gcacaagcaa agaaaaggga aaagttagat taaggataga aaggaccaag
     3721 cataatattt ttataggaac ccctatttaa ccatagagga tgctcacctt ctcaaatagg
     3781 aacaaagcca ccttagtatt catctttttc ttgataattt tctctatctt gcaatatagg
     3841 gtgcctgaga taataggttt tgacggctgg cttcatataa agatggcaga cctcatcagg
     3901 catgaaggat tcataaatcc agctccttat tcaaccatcc ttgtcgatac ctacgccgat
     3961 aaccagctag gtttcaggat cctgctgata ccttttacct tcctgggcct catccatgga
     4021 gcaaagatag cctctatcct atttgcatct gcagcattca caatattcta ttggtatcta
     4081 aggaaaaaca acagcataaa ttatccattt ttctggacag cagcttatgc ctttgcatct
     4141 tatgacttat tataccgctt tttagagcca agagccatgc ctcttgcaat cttatctttg
     4201 gtccttactc tattcttcat cgaaaagagg atgtataaat ccattttcat cctatctctg
     4261 ctatttggct ggctatatta tggatttgtc tttcagcttt tcattatatc ggtcttcctt
     4321 gccattgatt acctgaaaaa aagaaagctg gatatcaatc ttattctcta tccaattgca
     4381 ggaattgcgc tatcaatagt cataaatccc tattttccag caagcataag ccatctttac
     4441 acccaattgt tccaagtcaa ccttgtatca aacctttaca atgcagaatg gaaaagttgg
     4501 ggcatattag atttcattaa atttaactgg atttacatgc ttctatacct cacatcggtt
     4561 ggggcaataa tcaggaacca aaagtttgac aaaaagcagt tgggattatt cattatttcc
     4621 actatcttcc tggtggcgat gctaaagacc agaaggatgc atgaatactt tgcccctttc
     4681 tcagtactat ttgctgcctt ttgcctgggt aacaaagaga acaacagcca tgcaagatat
     4741 atttcagtcg ctgccttggt cctaatatcc ctattcagtc tggtatcatt aaatgaatat
     4801 atccacaaca accactttct gccatggtac aaggaagggg cagactttct aagaaccctg
     4861 cctgagggct caaaagtgtt cattaatgcc tatacctata cctatttctt tttctacaat
     4921 cccagtttag aatacacaca tggcatagat ttgacctatt cttatctaaa agatcctcaa
     4981 aagtttgaaa gatatatatc tgtcttgcag ggaaaagacc ctgggataaa tattgttaaa
     5041 gactatgatg caaattatgc agtaatcggt aagataagac aggacatcaa tctatttgat
     5101 tacatgataa actatcaaga tgattttgag ttgatttacg aagataagaa tttaggaatt
     5161 ctaagggtta aatagtggtt ttaaagagtt atagaaaaat aaataaaaaa taaaaaaggt
     5221 ttttatcctt tttattcaac tacagcgggt gtttctgtgg ttgcagcttc agttgttgct
     5281 gcagtcgcag taacttcctt aacagtgctt gtagcggtta caacttccat ctttgcgcct
     5341 tttagggcat aattgccata atcagctagg atagatgttg cagctcttgt gtcctttgca
     5401 tcataaccag ctacgatcaa ggccacattg ccacttcctg tctcaacaag ctcgattaag
     5461 ccttttccag cttcaaagcc tgctgcacag tctgttgggt tgctctttgc ctctgctgct
     5521 gctgcgttag cacatggtcc tcctactagg attagattct gtgccttcac gtcagttatc
     5581 tcgctagcta gctttgttgc acccacttct atcttgttca agcttactga ctcaacaccg
     5641 cttgctgaag ttccttttgt tacctttgca ccagctgttg ttatgtatac aaggggtagt
     5701 ctctgctcct ttggatactc aactgtcaac tgtgcagggt cgtttgttgg agattcccac
     5761 ttatattttg cacccatgct tgtataggca tacttagtgt tggtctcttc tgaaggtgct
     5821 acataagtga tctcatctgt tgtgtgctca acaaaggaaa cctcaccgct gcttgcagtt
     5881 atgttgaaag atgctattgc tggtacaatg ttgtcatagt ggttgctgtt tggcgttgaa
     5941 agagtaacaa tcacactagt ggtctcattg gtcaatacta ttttcaagcc agcatttgtg
     6001 ttgattgcaa caacatctgt atctgctgta acagttccat cagcatctag atcaactcgg
     6061 acatcaaagt catcagaggt atctgccgtt gcatttatta ctgcaaagct tgacccccct
     6121 atctttaatg tagcgtctgc tgtatcacca actgcttctg taaaggactg ctcgattctg
     6181 cttccagttc caaggttatc aaacttaact agtgctgtct cgccgcttgc aaccttgtca
     6241 gctcccttat atttcaatgc ataggtcttt ctgttaccgt catcctgtga tacgtctgta
     6301 acaattaagt aatcattctt cttaattacc tgcgtaccat taaggactaa tgtgttgtcg
     6361 ttgtctccaa acctagccaa gacagtggtt cctgaagtat atgcaatcgg cactgttgcc
     6421 aagttgccat ctccatcgac aaactgcagt tcataggtat cagaacctga tgtcctaatc
     6481 ttgtatgtct ctgtctcaac attgtctaag ccttcatact ttatgtccca taggcctaaa
     6541 aatgcctctg gctcttccat atactctgta aggctgtgcc ctgctgccac aaaaaagtca
     6601 tcgtctgcag ttatattaat ctgtaggctg tcaatgctaa atgttgcatt gtcatcgctt
     6661 ccagttatct tcacataggc atcgtcaatc ttttcagagc ctacttcaag ggcattacta
     6721 cttgcagtgt cttggattgc agtatctttc agatacacct tagaagctcc cagataaaac
     6781 tctactaagt ccgaagttac atctcctgct tcgttaggta ttatgtctgc aatgcccagc
     6841 tctgttccat cagataatgt agccgagtca ccatctttta aagacttagt ggtctctcca
     6901 ttaacattga acttggcata gattgtccca gtatcagtga ttgctgtgac agtcacttca
     6961 tacttaccat caaggtaggt ctttgtctct ccttcactta aggtgtcttt gacagaaccg
     7021 cccatcaagg ttaactctgg attgttgccg tttccatttg tccttcttgc cttgacaata
     7081 ttatattcct tgccaagaat ggttattttt tcatcctcaa ggtcgccaag ataggtacct
     7141 gtagaggttt ttgatcctgt gctgtcttca acatcacttt ttagtgacgt cttgaactca
     7201 aggctgtatc ttgcgatttg tacaccgctc ttaaagtata ggaaatcacc gactacatca
     7261 tcatcgtttt ccataaactt gacataaccg ccgttctttg cagtctcgtc aaagttgatg
     7321 tattgattat agcctgctgt tcctttctct gtggttatgg atccatctgc aagggaagtt
     7381 aactcatctt catcgatcga agtctgaatg tcccttatgg tctcattacc ggtagaacct
     7441 tgctggttgg tcatctcaag cttcttgctt gatgttcctg caagccatgc gtctccttca
     7501 acgcttgttg atgttcctgt tgatgttcca cttgttgcag cgtactgtaa gctcattgcg
     7561 atatcagata cgcctataac atcttctgct gctgctttgt ctcctacgac aagcatcccg
     7621 ctgaattttc cgcccgatat gaacggtgct ggatactcgc ttaggtcagc tgcggaagcg
     7681 atcattgtag ctcccaatag agttgttgct ccaatggcta caatcttctt tatggccttc
     7741 ctcaatttca tgtcctaaac acctccttag tgttctttac ttggctatct ttacataagg
     7801 atgcctagtt atacttatgg ctccttacta ctgtaatcca cctgtttcat atttaaagct
     7861 tacggtattt catacaactc agaaatatat attgcaccat ataaatggcc ttttttatca
     7921 tcggtaacca cctattgcct tatctttata aagtttattg cctggctcaa gctggtatgc
     7981 aaaagtatac tccttatgag cctcatccgt cttattcagg tattcatata ggacagcgag
     8041 attataatgg ctatcgggaa cagccggatt tagggatata gctctctcaa actgtgccaa
     8101 agcaaggtta atgcttccat cattggcata tatcaccccc agatccatat atgcttttga
     8161 taaggacggc tcaagttcta ttgccatgcc aagctcaagt atggccttgt caatttttcc
     8221 catcttccaa tagataaggc caagattgtt atgcccctta tatgacctat agattgataa
     8281 tgacttgttg aggtaatact cagccatact atagttgcct gcctcgccat atactgtacc
     8341 aagattgagc catgctacat ggtttctatc attcaatcga agggcaatga tgtattcctc
     8401 tgctgccttc ccaaagtccc caagtgtttg atatacttga ccaagatcat tatgagcaat
     8461 tgagcttttt ggctgtcttt caacagctga agataacagc gcatagtcac ttttccactc
     8521 ggcattcctt aggatagttg ctggcagata caaagcgact agcactaaaa ctacagcaaa
     8581 ggctgccttc ctatgattca gccgcgaaat aaggtaggac aggagcaata caaaccccaa
     8641 tgatgcgaca taaaggtacc tgtctgccat gaatgtctgc acaggaaaga tattggaaaa
     8701 tgggatcaat gcgatgaaga accagcagat tgaaaaaaat gcaagcctat ggctcttata
     8761 gcatttgatg caggcaaagc aaacaaaagc aagcagtgca aggcaagcag ccgcagtaaa
     8821 tccaagggaa gtatatgcag taacattata atccactgac aaccaaaaag gaaagagggt
     8881 tatgaagata tatctggcaa agatcactgc tgttgaaagt acccttgtta gggtgtctcc
     8941 cataaagaac acatcagtcc ttcctatctg gccaaggaca agatacctca aagacagata
     9001 tcctccgcta acaagaacaa taggcagata atgtttaagc catgacttag gccagacaaa
     9061 acataggtca taggctataa caaggatagg aaatattata gcttcttctg atgaaaaaag
     9121 tccaaggata aagaaagtga tagaaaatac aaaggctttg tctgagttgt ttttcctaaa
     9181 taagatatag ctataaaagg ctaaaaacaa aaacaagaca cccagcaggt caaatcctcc
     9241 tgtcatgtta gcaacccttt cagtatgcac cggatggatg gcaaaaagaa ggcccgcaac
     9301 aaaggcatag gttttatttt taaatagttg agaagctatc aaataagcaa ggatgcttat
     9361 ccctatgcta agcaggacag cattcaggtg gtatccaaac ggattcaatt cccatataga
     9421 gtaggtaaaa gcgtaaagca ctcctctcag cggcctatac agatttccgg tcgaaggccc
     9481 agcaaagaat tgagggatgt ttctaagcga cctgatctca gtattgtcaa cgataaagaa
     9541 ttgatcgtca aagacaaaag gattccagaa tgtgtttaga tacgcaaggg cagaaagcgc
     9601 gatgataagt atgacatgca actttcttgc tttcataaag cttgctaact aaagagatta
     9661 tataaattta tatcagaaag gaaggtgaat aagtatgcct gctccagcac catccattca
     9721 aaattaatca gtttagtgct cattaaaatc acccctgaat tctctgcaaa agctttaaat
     9781 aacaggtagg ttttatttag gcaaacatga taactgtcct tgaatacact aaggataagg
     9841 ttgtacaggg aaatttgagc aatatctgcc agccgaagac tttcacctgg atcgacgttt
     9901 ttgagccgac tcctgaagag atcaagcaaa tttctgatat atctcagacg cagctttctg
     9961 acttgaatat ggtgcttggc caggatgaga ggccaaaggt tatcgagata gaaggccctt
    10021 attcgctcat aatatttgga gcgcctgctt ttgaggaagg tggggttaag acaacacctg
    10081 tttacatata tacttcaaag ctccataatt gtgtcataac gattaggaat caagacacaa
    10141 aatctatcca caaactaaag gaggcaatag agtcaagaaa aggatttttt gacaaaggct
    10201 caagctactt cgtgtatcgc cttatcgatg agatcctaaa cacattctac ctcgtcttgg
    10261 aagacatcga aaataagata gatcagattg aagacagcat agtaagcgcc ccagatatca
    10321 gccttataag caagatattt gagataaaaa agacacttat ctatttcaac aagacgataa
    10381 ctgcaaatag ggaagtcatt gcaagcatcg agaaggaata cctgacagag atagataaaa
    10441 aaagagcaaa gcagtttcgc acattgtata acgataccgt ccagcttata gatatgtctt
    10501 cgacatacag agatatactt acaggcactc ttgataccta cctttcaagt gtctccaaca
    10561 acctcaatca ggtgatgaag actcttactg tcctgacatc atttattatg gtgcctacac
    10621 tgatagcaag catatatggt atgaatttcc agtttatgcc agagaccgga tggaaatatg
    10681 gctatttgtt tgccgtaggt ttgatgggtt tatccaccct tgggacatat ctttttttca
    10741 aaagaaagaa ctggatatga ttagcttggt atcttctagc tcaatatctg atagaaagcg
    10801 tcaaatatcg gcgtgtccgt cgtcactgta taaaacttca ttttaagcct tgcgcaggta
    10861 tcgtttatct tgccagcatg ctctgacata agcttttggt attgcatctg caggcggggg
    10921 cttatatgag tcacaagttt gtctcctgtc tcgctgtcct ttagcttgaa atcgcctgtc
    10981 atcttgagct ctttttcgac agggtcaagt acctgtatta tcttaacttc gtttgacccc
    11041 agcatgaaca acacttcatt tatctcgtct attggcataa gaaagtcaga tatgatgatg
    11101 accatcgccc tgctgcctat caatttcttg tattgatgga tggcatcttt gagcctcgac
    11161 ttgccatgcg tcttgatagc attcaggtgg tctatcatgg cagcaagctg tcccatgcct
    11221 cttcgaggct gaaagacaga aagctcttca gaaaaagtgg caaactggaa cctttcgttc
    11281 tccttcatcg caagatatgc aaatccagta cctatcatcg ctgcataatc aaacttactt
    11341 atgttcttcc catatcccat cgaggctgag aaatcgacca ctatatgcaa ggttagattt
    11401 ctctcttctt catatttctt gatgtatagg tcatctgtcc ttgcaaagac tcgccaatct
    11461 atatctctta tatcatcgcc aggcgcatag atcctatgct ctcgaaatat cagccctctt
    11521 ccgatagcat tgctttttct tggtccggta tacttagaag tgaccctctt gcttatgaca
    11581 aggttgaact tcgaaagctc tgctaaaaat tcagtgccta tcgccatatt gtcctacttc
    11641 acctttttca aaagctcaag gatggcatca tcgacagtaa gcccctttct ctcagcctca
    11701 aaattaagca caatcctatg tcttaggaca ggctgcgcca aagcttcgat atcttcagag
    11761 ctgacatggt ttcttcccat gataagcgcc cttgcctttg atgcaagtat aagtccgatg
    11821 gacgccctag gcgatgcgcc gtattgcact aggtcttttt ttgtccttgt tgcatctata
    11881 aggtcgatga ctctgcgctt aaggtcgttt gcaataggta tctcccttgt tgtctgctgg
    11941 agatgcaaga gctgctgctt gttgagtatc ttgcgcagtt tgttctcaac aaggggatta
    12001 gtgaacctgt ccacaatttt catctcctcc tcgggcttgg gatatcctac tttgatctta
    12061 agcaggaacc tgtcagcctg cgcttcaggc agtggataag tcccttcctg ttcgataggg
    12121 ttttgcgttg caaggacaaa aaaaggcaga tcaagcttgt aggtcgagtt gcccacagtc
    12181 acttgctttt cctgcatggc ttcaagaaga gcgctctggc tttttggtgt tgccctgttt
    12241 atttcatctg caaggactat gtttgaaaag ataggccctg gctggaactc aaactcacga
    12301 cggccgcctt tctcactgat tatatatgtc ccggtgatat cgcttggcat aagatcaggc
    12361 gtattctgta tccttgaaaa cttcatatca aggacctcag aaagagtcct tattgtaagc
    12421 gtctttgcaa gaccaggata cccctcaagg agcgcgtttg catcacaaag cattgcagtc
    12481 atgatctggt cgatgagatc ctcctgcccg attattaccc ttccgacctc cttttggatt
    12541 gtgtcgaaaa cgatcttata gttctttacc atatttacca ccttctcatt acttcacatt
    12601 gaaatcgttt ttgagcatgc tcagaaatcg ccggcttact ctgagctaaa gcatcagagc
    12661 gtgcgcccaa agcgatttct gacatgtcga aaacgatctt atagttcttt accatttgtt
    12721 cacctcactt gcttatctcc ttaaaataat ccttaaccag ctgctggttc tctacagtga
    12781 tcttttcttc atatgctcct gatgatactg caaatatctc atcagggaat gtctcttcaa
    12841 actccttatc agggatagtg ttttcatcag tgccgattat ctcgacagat ggctgcctta
    12901 tcttcacctg ggtctctgag tttcccagtg cagccatgcc ctgctgccca aatatgtcat
    12961 cctctccttc accttcacct gctgcaagta tgtttccttt tccgtcagat acccctccgc
    13021 cggatcccct tataaattca tctgctttgt caaggactag gcttaggtcc ccgaaatgca
    13081 tattatatat agaggcaaaa agtatgagca agcatagtat cacactgcca agaactttgt
    13141 aagagactct tgacagcttt acaaaagatg aaacattgac gtttgatagg tcttttgtca
    13201 cctcttcctc aagttcctcg accaccatgt tgtccaggtg cacattatca gctgctgtcc
    13261 gaagcttttc gtacaatgga gcgtacttat cctctactat cttatacttg ttcttattca
    13321 tgtctatata tagtaggaac gcaagatatg caagggtagg aaacaaggaa taccagggat
    13381 tgagattgaa catcataagg aagaaataaa agatgaggaa tatcaataca ctggtgagta
    13441 tgatatcaaa ggccattatc tcatctatgg ctaaattgac ttctttgagc acctttctaa
    13501 aatctttcat ataatcctcg ataactaact ttcatcctcg catatgtcca gcatattctt
    13561 ataataatcc ttatcttcct tcatgcgatc atattttgtt gtcatatcag caagctggct
    13621 gcttaagctt gtcacctgct ctgtcttctg gccaagctct gcccttgtca ccactaatga
    13681 ttccttatag ttgttccttt ctgtctcaag ggtgtccctt tcgtccttaa gctcagtata
    13741 tttctggcca agctcttctc ttccttgttt tgtggtgtta agttcgctgc ttgtctgcat
    13801 caacaaagct tttttctcaa gcaggtcctt tgttaccttg tttagctctg caagcttggt
    13861 gttatattca tcgttcatat tcctgaaaga tacttggtag taggcggtaa accctgcaaa
    13921 gcttactact gtcgctatta tcaatatgaa aaacacaaaa ttcacgtctc tctttattat
    13981 cgacattttt ttcacctcat gagattgatg ttctgcctta tccttcttat cagtatatcc
    14041 agcaaaaata ttatcagggc agccattatg aagggccagc ggtaatttat agtttcgacc
    14101 tttgatcgtc ttgacatgct gattatcgtg ttttttatcc gctctatctc cgatggggca
    14161 aagacctctc cgcctgagta gcccaccagg tctttgagtt cagggttaag ccctatcttt
    14221 agatattcat cgttgtaatt gacagcaagg gtcgcatcaa gtatctggta aaatcctgtt
    14281 tcatttggca tgaatttggc tgaataaagc cctgtatcta tcttggcaaa ttcaaggcct
    14341 ttgtattccg gtgctgcttc agatattacg ttgatatctg tcggcttgcc cagggttgtg
    14401 tcctttgccc ggacgtcaag ctgcatgtcc ttggtaaagt cccctatcgc ccaattgacc
    14461 gccctcatca gcacttttga attctgttca gagtaaaatc ctcctgacca tttgcttcca
    14521 tcgtccgtag ataatacagc gatccttcca aggccatacc ttccggcggt aagtatcgtc
    14581 cttccgcagt ctgtcgcaag aagctttctt ccccagcttt ttggcaccac cagattatac
    14641 ccgccgatgt ttccatttat accctctgtc cccttggtta tccaatgaga agaatccatg
    14701 acaagaaccc ttccgcttgt tgctttttgg catctctgct cctcctcatc tcctataaac
    14761 attatcttaa gtttttcagc ctcgtctggc tcaaagtaaa gtccgccccc aagctcagcc
    14821 atatgctgca gggtgtctct tttgacatta tatcctactc cgacagtata tacctttatg
    14881 ccaagagagc ccatagtcct tatctggtcg tcaagatagg tcttgctctc cccataatct
    14941 ccatccgaaa gtatgactgc gttcttgctt cctttaaggt catataatga ttgccttacc
    15001 ctcatcaaag caggcccgat ggctgttcct ggaggttctc cgtttgggcc gtagggtatc
    15061 tgcagctggg agatcaattc gtagaggtta ggcatctcta ttattggttt tagtatgcat
    15121 tgcgcctctt gaggtacaca gtatcttttt gtagtatgtc ctatgatgcc tatcttgtcg
    15181 tccggattga ggccgtttat aagcccggtc atcagcgatt tttcgacatc aaggaccgtc
    15241 tcagggctgc ctgatttaaa aggcagcgaa acagatcctg aaaggtctat gatgaaaaat
    15301 atgttgtaga tctcttccct ttctcttttc ccaacaccta tatctacagg aagcatgctc
    15361 tcgaagtatg agcccttata gcctccgttg tcaaatgagt tctgcccccc aacgattatt
    15421 gcccctttac ccttttcact gagataggcg ttaaggaggt cgactttgtc gctgcctatc
    15481 ttgtcgatct tcaggtcgtt gaggatgact gcatcaaatg ttaagaggtc gccagggatg
    15541 tcggtctggg cagtcacgtc gtatatcctt tcgagtgcac tctgaagagg cccggacctc
    15601 tcgccgacat aaagcagctt tggtctgggc agtgatttga cggctttata gtatatattg
    15661 ttctgtataa aaagatcctc ttccggaaag agtatcctgg ctgtgatctt gtgatagcct
    15721 tttgaaaatc tccagttaaa gctgccatcg cttccaagct ttatctcgtt tccatcgaca
    15781 aatacctcca gcttgttata ttttacacct ccaagattgt tcatgcgtgc ataaaatgtc
    15841 gtctcgcttt gctctatcac ctgtgaaggg ccgtctataa acacagctat atcttttttc
    15901 agaggtttga ggtcaagtgt atggacagtg gtgttaagga cgctgctaaa aaggatggca
    15961 tctcctatat ccctgccata gttgttgttg ccgtctgaga tgacaagcaa atggtcgtct
    16021 ccctctgcgt tctggagtat cccgtctcct atggcagagc gctctccata tgctgaagtt
    16081 ttaaggtttg ttggaaaata ccttgaaagc tcatctctaa ggcttcctgc gatactcgtg
    16141 ttaaaaacat caaagcttgt ggaattgtct gcaagtattg taagtgatgg atttccctcg
    16201 gtcactgttt cttgggttgt atagggggtc gctatggcta tcagaagcag cacaaaagct
    16261 atggtcctaa gcacaagaaa gattatcctg tcaagcctgt ggctctttgc aaacgctttc
    16321 ttctcctccc ttttcttgaa gcccgcaaag tttatccata ggataatgcc catgatgata
    16381 gctaggggca cagctagata aagatagaaa ggatctatca ggcaatactg tgagaagcaa
    16441 accttgccta gtccaagcat cataggtcac ctctcctttt tatataaaga aattcgagga
    16501 ataaaagcag gacgcccgcg ataagcagcg gcacttcgag gtcaaggttg cttgtatctt
    16561 ttactttttc ttctgatatt tcacctagtg ctgatttaag ttgtagagtc tctttgttta
    16621 tatcagactc tttctcatct cccaagttta cggctatctt tttgccgtca acctcatata
    16681 tccctgtctc atcgagcaca agtctgcttg cagagatcct cgatgaaggg gttttgatct
    16741 ctgggcttgc agcgcttact atctttcctg tcttatagtt gaagtctttg atgttctctg
    16801 tcttcataag gtagtttaca agattgttcc aaaatatcgg atagaaaggg tccgagtaaa
    16861 agccggaata tttgtcgatg atgccgtagt atattacctc tccattgccc tgcctcttga
    16921 cgacgatagc aggagaaccg tcctctttgt tgctggccat gaccagtgtg ccgttctggg
    16981 cctctgcgct tatgtatttt gaggcgctgt taaagcatgg ctcgtcgctg aaagggtctt
    17041 ttggaaaaag gttgccgatg atatcagcgc agatgcttgt ctgcctgtcc tcctttgatt
    17101 tcacattgat gggcagcagt cctgccatgt ttatctcacc taggtcttcc tgagctgata
    17161 tgatgagctt tcccccctta tctacatact tcttaaggtc ggtaaaatcc gtaggcacaa
    17221 atagatcttt ttttatctct cctatgacga ctatgtcatg atcgatgtta taggcattga
    17281 ctgtcggcgg ctcccttacc tcgagttcag ccccgatgac gcttagagct gatttgatct
    17341 tggattcatc agggttgttt gcgatgagaa ggatgcgaaa agctttcttc tcaggtgagc
    17401 tgacaaatgc cttgttgtcc accaaaaagt catcgttgac ttcaagagta agctcgctta
    17461 acccgcctaa agtttcaaac agcacctttt cttttgattt tgcaagaagg cttaactctc
    17521 ttgaatctac cttccggcca tccttgctca gcgaaaccct gattttttcg tcagaatctt
    17581 tatagttctt tatctcgatg atggtctcct ttttgtcgac gaatatgtca gtgatgccta
    17641 tgttttctgc cttgctgttc acatctatca ggttgacaga atgtcccctt gacgctagct
    17701 ccctctttgc gataaggatg tctgaccctt ctgtctggat aaaatcactt atgactgtta
    17761 tgacgccttt cttgtcgcca agcatgtctc cggccataag catggcgtct ccgatatttg
    17821 tggtggtggc tttgggcact atcctattga gtaagttaag tgcattgcct gagctgccat
    17881 gctcaagtac tatagcaggt gtgctctggg caagtatgat tgtgtttttt agcccaaggt
    17941 tgtcttttgc cttgcctaca gcctgctcaa accttgtctc catgccgtcc tttgcctgca
    18001 tgcttgcgct gtcgtccaga actatcacgc tgtttctgac catgatgctg tgcgggatct
    18061 caaggtaagg agatgctgca gctatcgcaa gcatgatgat gatggcaagc tgaaggaaaa
    18121 aaagaaggtt catcagcact cgctgcaaaa aagaagcttt gattagcttg tttctttcct
    18181 tcatgataaa cattaggctg ggaatcaccc tctcgaaagt ctttggcctt ataagatata
    18241 ttatcacaaa aggaactatt gcaagcaagg catagagccc ggcgatattg ctaaacacca
    18301 ttgcttcccc tctttttcct gagcactacc tctaggtcct ttgggctgac aagctcggtc
    18361 actgctataa ggtttttgcc aagctcatac caatagatat cagcattggg tgatatgttc
    18421 aggtcctgcc ttgcttcgtt agggagcgtc acctgcccct gctttgtgag ctttgctttt
    18481 ccgacaaact ttggttccat attaacatta atagctttaa tatatataaa gctgttactt
    18541 tttttattat atctaagtaa cttattaacc gctggattat aaacctttcg gtaaagcaat
    18601 acagccaggc aaacccggca acatacagaa ataaaatgaa agtattttta cccgccggta
    18661 ggagagatag tgatagcaaa ctaatctctg tttaatgcag ataaatgctt agcaatacgc
    18721 atgtcagtaa agagattaaa tggggcatat ttcttattat atctgttgga tatattatct
    18781 cgttcacaca caagataaca tttattcggg ccctctttat actgcatgat gctttcgcag
    18841 ttcataaaag ctggcagggt taaacctcct tttaccatat tctgaggtat ttcactacca
    18901 taaaaaactc cgcaatattt aacaagcata ctatctgtaa gactgtgata gccttgcatt
    18961 aaaaggtggc ccatttcttc tgaattggct actgcatggt tctttctaaa aaattgccgg
    19021 atacttgtat ctatgagaac ataggtatcg ctacccttta accttgctat aataaaaagg
    19081 tgtttgctgt ctttatggat atagaaaaat ctccccataa tatcagagtt ctgaagccca
    19141 tacagctcta tctgcccgca atttaaagaa aatagccggc ttaatacgtc cgcttgagca
    19201 aagttacagg tattaaggac accgttacct gatagataga ggtatggttg tgaaagattt
    19261 gctgcctccc gataatccgt gggagagttg cttggatcca ttaaaaaagt cctgttttct
    19321 gcaacgactt gctgaattaa acacgccaaa tccaggtcat ctacagccat tcctgtcaaa
    19381 aatttatttg ctacaatcct ctgtgattcg ggaaaatcaa ccattggcaa cggaccctca
    19441 tctttcatta aagcaaatgc agctcggtct acctctgtgg ataagtcctc atcaactggt
    19501 acgccacctt ggcctgggtg catgcagtta aagaatcaaa agagattatt aagccttacc
    19561 tatttatgcc tcacgttacc acccctttcc ataacaaagt tttaaataaa cgaaatatcc
    19621 aagccttcct aatggcaaaa ctgtcaagga tcgaggttat tctgctcatg ttccttgcga
    19681 tactctttgg cgcagtcttc ctttttgctt ttcttagcgt gatatatgga tactacctga
    19741 tccagatagg ccttccccag atagtgatat ccattgccat cttgctcttt gcactcgctg
    19801 ccttgctcaa agtgtcatgg catatagttt cttactaaaa atttataaat atactaagaa
    19861 atctttcatc ccatgagaat tttaacgctg cattcagact tcatcgagtt tgaggcaaag
    19921 aaaaaggcga taaaagagcc tgaagagctt aagaataagc aggagaggat agaagaatgc
    19981 cttgtagttt ttatttctgt tgaaaataag gacgaatcag atccagcgtc agcggcagca
    20041 aaccttgtca agaatataaa agagatagct gccatggtaa acgaaaaaaa ggtagtcctt
    20101 tacccttacg tgcacctaag cagttcgcct tcttcaccag caactgcact taaggtgctt
    20161 gaccttgcag aatcagggct aaaaggagaa tatgaggtcc atcgttctcc tttcggctgg
    20221 tataaatcat ttaacatttc tgtaaaaggc caccctctat cagagctgtc cagagagttt
    20281 gggcccgaga aagaagaggc aaagccaggc gaagcaaaag aagcaggcaa agaggaagag
    20341 acagaatcgc taaaaaaaga aaggaagctc acttcctatt ggcacatcct gagtcctgat
    20401 ggcactctca gcgatgtaag caagtttaac tatcaaaatc atgaaaagct caagaaattt
    20461 gcattctatg aaaaggagaa aacaagggca gttgaccagg agccgccaca tgctgtgctt
    20521 atgagaaagc ttgagcttgt tgactatgag cctgcttcag acggcggcaa cctaagatat
    20581 tatcccaagg gaaggctaat caagaaactt attgaagagt atgtcacaga tagggttatc
    20641 agttacggcg gcgttgaggt agagacgccg ataatgtatg atatgaacca tcctactctt
    20701 tcaaaatatc ttcagagatt tccagcaagg cagtatcaga tagagtcaga caaaagaaag
    20761 ttcttcctaa gatttgcagc gtgctttggc cagtttttga tggctcatga tgcaaccatc
    20821 agctataaag accttccatt aaaattatat gagttgacaa ggtattcatt cagaagagag
    20881 cagtcaggag aattgacagg attaaggcgc ctgagggcat tcacaatgcc tgatgtccat
    20941 gctatggtcg cagacatgga gatggctatg agcgaataca aggtcaggtt taatctatgc
    21001 atagatgtgt taaggaagat gggcatagag ccaaaagata tggagatggc tttaaggaca
    21061 acaaaaaaat tctttgaaga aaacaaggag ttcatgcttg gccttgtcaa tcatttcgga
    21121 aagcctgtgc ttcttgagct atgggacgaa aggatattct actttgtctt gaagtatgaa
    21181 ctaaattttg tggacaacct aaacaaggca tcagccctga gtacagatca gatagatatt
    21241 gaaaacggag aaagatatga catcaatttt gtggataagg aaggaaacaa gaaccatccg
    21301 cttatacttc attgctcgcc ttcaggagcc atagaaaggg tcatttatgc tcttcttgaa
    21361 aaggcggcgt ttgaccaaaa acagggaaag actccaaagc ttccgctctg gctttcacca
    21421 actcaagtaa gggtgatacc tgtaactgaa gagttccagt catatgcaga tgagataaca
    21481 gactgtttga gaagcagcag tataagggca gatatagacg accgtatttt acacgtcgga
    21541 aaaaagatac gctcagcaga ggttgagtgg gtcccattta tcgttgttgt cggcgaaaaa
    21601 gagaaggccg caggaaacat caacgtcagg attagggaga caaaagagca aaaagagatg
    21661 tctgtaaaag accttattga caagataaaa tcagtgacag aaggtatgcc gtttaagcct
    21721 cttgccctgc caaaagagct atcaaaacgg cctgtgtttg tgggttaaac gttttttcaa
    21781 gatgattgcc ccaggattgt ttatgatcca cagaataacc tgtgcaggta tgtgccgcga
    21841 tcccagtaac ttttaaatac ttcaacctta aatttatttc ttgggtggta tctatgaaaa
    21901 aagaatcctt tagatggcta gtggttatag gagccttgat aatccagctc tgtcttggtg
    21961 ccatttattc atggagtgtc tttgtcaatc cactcaaaga agcttacggt tttacaacta
    22021 cccagaccca gatcattttc tctgttgctt tggcgacttt cgcaattaca atggtttatg
    22081 caggaagatg tcaggataag gtaggtccaa gaaagattgc tatgatcggc ggtcttctgc
    22141 taggcatggg ttatatggcg gcaagatttg caggttcatc tttctcggga tatttattga
    22201 cgataggagt catcggagga gcagggcttg gttttggcta tgtctgtccg atagctgcat
    22261 gtgtcaaatg gttccctgac aaaagaggac tcatcaccgg ccttgccgtc gctggctttg
    22321 gtgcaggagc atggctcttt gcacagatag caagcaatct tatggaatct attggcattc
    22381 ctgcaacctt catgactcta ggcatcatat ttctgatagc gatattcttc agttcccttc
    22441 tccttgtaaa tccacccgaa gcttgggtcc caaaaggatt taaagtagaa aaaaagaaag
    22501 caaaggctgc tgtaaacttc accgcaaggc agatgctaaa gacaaaacag ttctggttcc
    22561 tttggctgat gtttaccttt gcagcctcgg ctggattgat ggtcataggc aatctaaagc
    22621 cgtttgggat aaactcgggt ttaactgcag cccttgcagg ctcagcagta ggcatccttg
    22681 cattattcaa cggtgcagga aggataacct ggggttttct atcagacaag ataggaagga
    22741 caagggctat gactttcatg tttatcttgc agggaatgat gatgctcatc ctgataaaga
    22801 tgggagcaac gccgctgaca ttagctatag ctgcagcatg ggtcgggttc aattttggcg
    22861 gaaactttgc acttttccct tcagccacag ctgatttctt cggaacaaaa aatctgggga
    22921 tcaattatgg atgggttttt acaagctatg gtgttgcagg catacttggc cccatacttg
    22981 gcggaaggat ctttgacttg accggctcct atctgtatgc gtttatgcca tcagggatat
    23041 tatgcatctt ggcagcagga cttgctattg caacaaaaag accagccgca caggcagctg
    23101 caagtgcaaa agcagcgcct gactaaattt tttttattac aaatgcaaag ggctcagtct
    23161 tccagacaag ccctggatta tcctttacat attcgattat tttctccttt ttgatataat
    23221 tatagaactc caaaactctc cccttgaact ccccttcgct cattttacga aggctagggc
    23281 atatcttctt ctcaaagcaa gctatcacaa gctgcccaaa tacatttttc atgaagataa
    23341 aaggccatat attgcagcac aatggctttc tatgctgtat gctgcaaaga tgatttgcat
    23401 caagaaaggg acattgccag tttccctcgc taaacacaag cttgggctta tacagattgt
    23461 tagacaccaa aataaactcg gcatcgagcc cagcaatctc ttttaaggta aaaacaggag
    23521 caaacctggt ttctttccgg tcaaatatac aacaaccttt gcacctctgg caatcttctt
    23581 tttttgcgat ttgcatacca caatgtaact ttaccctctt attaatctaa ccggctttgc
    23641 gcacaaatta tccctgaatc ctgttaaaat atgatagtct tgccctgtta tgcaagatta
    23701 tccgagaaat caattgaaca aacttacaat ggccccttac cttgctgatt ataccaaaga
    23761 cccttttgat tg
//
LOCUS       DASRUK010000129        11909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2119742, whole genome shotgun sequence.
ACCESSION   DASRUK010000129 DASRUK010000000
VERSION     DASRUK010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 11909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11909
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2119742"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2018)
                     /locus_tag="VFF28_05780"
     CDS             complement(<1..2018)
                     /locus_tag="VFF28_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45164.1"
                     /translation="MAKKKQAVKETIRYIVKHRVFIISAIVLLSLALILFGAKFWLYF
                     RFILGNDTVVTLEANKRNLYLVHGQNAELEFQAKVATNPFCRALCTYSFKDISAGTTI
                     EEDSFILRSSSPLKKAYPIIPPGIGTGLELYRFDIDCYSAKTVFCSTAGEPSTRSILV
                     TVNYDLTVEEKRLKENLAGSIKTLTHKASILNGMQKTLGDLAGELNKTVLFEDKSLET
                     SQSISLLAEKLLYLQGIWDEEDYISLEASLQDLDLPDDMFKEAEGNLSSLLKSYNRII
                     DENENERNALENIARQPIADLTLSESIRELIDDFNSEALMLKQRTALAEKEALQQELS
                     KKRLEVGLAVSELIKKKAIGLEIETSLDSDLLCNLTSNCISHTSIAELANASSFNLTE
                     VCSKRQETVQAISSANSSSLMQDYPSDSGFWDDMDAQLDNMKRFYAAGYLLQLPKNST
                     NSRIIIDSLNRSFREAAPHPEYNLTSALIKALAEQKPVACNLTETTYPEIISPGLDRI
                     ELPEYPVIPLEVVFKPQTPRCCVFGKCTSCCMTQECRDDSASYPVVFIHGHAIDKETS
                     AEYSLEGFNKIQDKLEEDGYLNAGFIALNTLKDEPPGLWGLPRAPITVRTTYYYDVLG
                     DPENYMVIQTKSQNIDTYALRLRELIDTIKYKTGKPKVNIMAFSMGGLV"
     gene            complement(2050..2592)
                     /locus_tag="VFF28_05785"
     CDS             complement(2050..2592)
                     /locus_tag="VFF28_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45165.1"
                     /translation="MQLLAPCRIARLEHVAHQAAKDILFSKAYNAEIELTVKYTADPA
                     IAMLQVIYRRNSAILVQGEDILRTDSDDLPQIVTDSFETMEKIWTAMGIHGGRKVIPY
                     HVKYHLERQRREPVFYSPEQCGIILQKAGLVKNPLFLPRRPRYTLQEIADILQMTHEF
                     AEDKAQKAAYDGALNLVINN"
     gene            complement(2650..3030)
                     /locus_tag="VFF28_05790"
     CDS             complement(2650..3030)
                     /locus_tag="VFF28_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008082772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8e"
                     /protein_id="HZX45166.1"
                     /translation="MVVFKGRGRRSVRGSRLKAYRFYRKYELGRLPAFTGLGAKRLKV
                     IDTLGGNSKLRLLSEEFANILDQKTGKSKKGKIKTISGNPANRHFVRRNIMTKGAIIE
                     TDLGKAKITSRPGQDGVVNAVLIS"
     gene            3159..3383
                     /locus_tag="VFF28_05795"
     CDS             3159..3383
                     /locus_tag="VFF28_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LSM domain-containing protein"
                     /protein_id="HZX45167.1"
                     /translation="MEASRPLDALNRARDKKVIVELKNQKQYVGNLKSFDIHINVVLE
                     NAEERIEGEVKRKLGVVFIRGDTITVISPE"
     gene            3388..3552
                     /locus_tag="VFF28_05800"
     CDS             3388..3552
                     /locus_tag="VFF28_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37e"
                     /protein_id="HZX45168.1"
                     /translation="MTKGTPSMGRKSGRRNMIHCRRCGKRTYHISKKKCSSCGYGRTA
                     RLRKYNWNKK"
     gene            complement(3576..4274)
                     /locus_tag="VFF28_05805"
     CDS             complement(3576..4274)
                     /locus_tag="VFF28_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45169.1"
                     /translation="MKKVWLILLVIAALTLIASCTKDTSCSTLKGPSKDDCYFEKAVS
                     EISLDGCDAIESKVLKDSCVAEIGIKNSDLSICNSIEGKSKDYCISKIALGKKDLELC
                     KSTDTEYWSDVCYSSIAVSTNKTSPCANVSNTMEKDKCYDTISRVTLDWQGCDLIRND
                     TVHDLCIRKVAITLNQESLCEETRSVVVGDICYQKIAQAKLNISICEGASYREIRAAC
                     QAAVRSLLNKTNST"
     gene            4365..4448
                     /locus_tag="VFF28_05810"
     tRNA            4365..4448
                     /locus_tag="VFF28_05810"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4399..4401,aa:Leu,seq:tag)
     gene            4476..4892
                     /locus_tag="VFF28_05815"
     CDS             4476..4892
                     /locus_tag="VFF28_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HZX45170.1"
                     /translation="MKSHTKYLQFNTNNKIDFVNITSQVEDAVSESGVKEGLVLVNAM
                     HITSSVYINDAESGLISDFKEWLKKLAPTDINLYKHNLTGEDNGYAHLWRTIMGREVV
                     CAITGGKLDFGPWEQIYYAEFDGQRQKRVLVKIIGE"
     gene            4984..5190
                     /locus_tag="VFF28_05820"
     CDS             4984..5190
                     /locus_tag="VFF28_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45171.1"
                     /translation="MLGVVENHKARKGGGFFFTFFGIFLFTMPKKCIICGECAEFNIK
                     GSSECYCKGCASDSFEDLGYLEEL"
     gene            5457..6215
                     /locus_tag="VFF28_05825"
     CDS             5457..6215
                     /locus_tag="VFF28_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HZX45172.1"
                     /translation="MIENSFIFLDGIREKTEQNIWNQGIYNWDSFIQAKAVKGIGNTR
                     KCYYDRQLEQARKELHDYGFLNRFPLCETWRIYDYLKDECVFLDIETSGIGKHCDITV
                     VGLFDGIETKSMIAGINLDLRALKRELERYKMIVTFNGAAFDIPFIKKRYPSLLPEVP
                     HLDLRFACEKAGLSGGLKKIEKELGIARNKLIDDLYGGDPYLLWRMFRGSGDEHYLNL
                     LVEYNEDDVFNLKKIADYACDKLKSDILKTMYEP"
     gene            6254..6523
                     /locus_tag="VFF28_05830"
     CDS             6254..6523
                     /locus_tag="VFF28_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45173.1"
                     /translation="MTDKPTARQELHRILTQIVRGEYPRLGFNSSKPRSFEGTHIGVA
                     MDSIDFTVYPNDPFKFNGGLIAQVVQVVGKYGQSGQYVIKFYYLH"
     gene            complement(6524..7201)
                     /gene="nth"
                     /locus_tag="VFF28_05835"
     CDS             complement(6524..7201)
                     /gene="nth"
                     /locus_tag="VFF28_05835"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HZX45174.1"
                     /translation="MNSEKEIKRAVISKVIAILKKESVNWKDPFVSHWARVEKNPFTT
                     LISCILSLRTKDDVTAKASTKLLIKYNTPEKIAKLSAKQIEKLIFPVGFYKTKAKRIK
                     ELSETLISKYQGTVPGTFEELINLKGVGPKTASITIVYGFNKPDHIAVDSHVFQITNR
                     LGWVSTTSPEKTQPELEKIVPRKYWYDINELLVKFGQNICVPVSPFCSKCPVRKYCPK
                     IGVGRSR"
     gene            complement(7198..7884)
                     /locus_tag="VFF28_05840"
     CDS             complement(7198..7884)
                     /locus_tag="VFF28_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009627758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HZX45175.1"
                     /translation="MIKDDMQKLANPKKAKILGRFFKTGKGEYGEGDIFFGIIVPEQR
                     KLAKKYLHLSLEEITPLLKSRIHEHRLTALFILVSKFKKAGKKEREQIFDLYLSNTKN
                     INNWDLVDLSAPNIVGCFLLDTKRTILYRLARTGSVWERRLAVMATFAFIKQGDYRDA
                     LEISKILLDDDHDLIHKAVGWMLREIGNRSLPEEEIFLKRYFRQMPRTMLRYAIEKFP
                     EEKRKQYLAR"
     gene            complement(7881..8486)
                     /locus_tag="VFF28_05845"
     CDS             complement(7881..8486)
                     /locus_tag="VFF28_05845"
                     /inference="COORDINATES: protein motif:HMM:NF002305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosylase/DNA lyase"
                     /protein_id="HZX45176.1"
                     /translation="MDIYLLHSSYKERKDIIKQRLSEFSKVKGGDIFYELCFCLLTPQ
                     SNALKCDECIQLLKNKKFHKNSFDTKPFLRPRTRFYKNKSHYLDLSKAQYKEIERNLD
                     ELKDPKELREWLVKNVKGLGYKEASHFLRNIGYSGLAILDRHILKNLLALGVIDNIPS
                     LTKKNYLALEEKFNNFSRQIKIPMDELDLLFWSIQTGKVFK"
     gene            complement(8487..8699)
                     /locus_tag="VFF28_05850"
     CDS             complement(8487..8699)
                     /locus_tag="VFF28_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HZX45177.1"
                     /translation="MAAEKITKEMQFSELLEKYPKAAPILMKYGLHCIGCHIAAFETI
                     EQGSLAHGMGDEDIKKMLKEMNAAVS"
     gene            complement(8700..8888)
                     /locus_tag="VFF28_05855"
     CDS             complement(8700..8888)
                     /locus_tag="VFF28_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HZX45178.1"
                     /translation="MAYKIEVSDECIGCGACAAQCDNFEIDGSKAVVKEAQVAELGTN
                     QDAVDACPVGAIKIKKLA"
     gene            complement(8901..9245)
                     /gene="eif1A"
                     /locus_tag="VFF28_05860"
     CDS             complement(8901..9245)
                     /gene="eif1A"
                     /locus_tag="VFF28_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor eIF-1A"
                     /protein_id="HZX45179.1"
                     /translation="MSKKKEEAARQEELQEQISRIHLPRGRQVFGVVQRRLGGSRMEV
                     KCFDGKTRICRIPGRLKRRLWVREGDIIIVEPWEFSGDDKGDVIYKYTKAQVEYLKRK
                     GYLKQIEDFEEF"
     gene            9498..10010
                     /locus_tag="VFF28_05865"
     CDS             9498..10010
                     /locus_tag="VFF28_05865"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HZX45180.1"
                     /translation="MARKKGLNLPKERTFSGPASLWKRIAAFMTDLLIIDIVILFPLN
                     GVVSRLIPKFSSFSEAVEVFSSGSYNSGIIPLAFMMAILFFLYFTWMEYKIKQTPGKM
                     LFRISIKSDLPDMKLWQAVTRNIFAVPIFPFFLLGIMDFVFLLFNKTGQRLSEILSKT
                     RTVEEYPLDG"
     gene            10003..10908
                     /gene="sppA"
                     /locus_tag="VFF28_05870"
     CDS             10003..10908
                     /gene="sppA"
                     /locus_tag="VFF28_05870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HZX45181.1"
                     /translation="MAESNWGTAIKIILVLVVLSFIAAAFLSVSIGEDNADGNIAIIP
                     IKGVISTEGSGLFDQQVASSAEIVSLIEKASKNPEIKAIILEINSPGGAPVATEEIAN
                     AIESTDKMTVAWIREIGTSAGYWVASSADKVVASRMSTTGSIGAIMSYLDFSGLLDDY
                     NVTYQRLVAGQYKDIGSPFRHLTINEQQIMQKYLDRMHEYFIKAVAINRNMTVEQVRE
                     VATGMFFLGEEAKELGLVDELGGKAEAVKIIENQLNITAVTVQYSHEPTLGEILGDVF
                     SKQSFYVGKGIGSSLLDAGSSKIRI"
     gene            11153..11401
                     /locus_tag="VFF28_05875"
     CDS             11153..11401
                     /locus_tag="VFF28_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45182.1"
                     /translation="MPPGLNYAGVLLLALIITGCQEVECNKDSDCFPAECCHPTSCTN
                     IKPDCDGRFCSMNCAPGTMDCGQGYCGCVDNKCSAVIQ"
     gene            11398..>11909
                     /locus_tag="VFF28_05880"
     CDS             11398..>11909
                     /locus_tag="VFF28_05880"
                     /inference="COORDINATES: protein motif:HMM:NF017709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I9 family protein"
                     /protein_id="HZX45183.1"
                     /translation="MNKFAIVFLLIVASNIVFAGKIDPLVEQELEENGEASVIIVMNE
                     PAGKTFLNAAGKDNRKRENVIASLSEDSKLSVKHKYSAINAFSARVSDRSIEKLSDDP
                     NIKSVHLSKRYYATLDVSVPLINATSTWGISIGDQNITGAGQTVCVIDTGVDYNHTSL
                     GSGWGNVVIGG"
BASE COUNT         3231 a         2433 c         2532 g         3713 t
ORIGIN      
        1 acaaggcctc ccatgctgaa cgccataata ttaacctttg gcttacctgt cttgtatttt
       61 atggtatcga taagctccct taatcttaaa gcataggtat ctatgttctg gctttttgtc
      121 tgtatcacca tgtagttttc agggtctccc agaacatcat agtaataagt cgtcctgaca
      181 gttatcggcg ctcttggcag cccccataat ccaggcggct catcttttag agtgtttaat
      241 gctatgaatc ctgcgtttag atagccatcc tcttcaagct tgtcctgtat cttgttgaag
      301 ccttccaggc tatattcggc agatgtctct ttatctatcg catggccgtg gataaatacc
      361 accggataag aggcactatc atccctgcat tcctgagtca tgcagcagct ggtgcatttt
      421 ccaaacacac agcaccgggg agtttgcggc ttaaaaacta cctccaacgg tattactgga
      481 tattctggaa gttctatcct gtcaaggcct ggagagatga tctctggata ggtagtttct
      541 gtcagattgc atgcaactgg cttttgctct gccagagctt taatcaaagc agatgtgagg
      601 ttatattcag gatgaggcgc tgcttctctg aatgatcgat ttaatgaatc aataattatc
      661 cggctgtttg tgctattttt tggcagctgt aaaaggtaac ctgcagcata gaatcttttc
      721 atgttatcta gctgcgcgtc catgtcatcc cagaaccctg aatctgaggg ataatcctgc
      781 atgagcgaag atgaatttgc tgaggatatc gcctgcactg tctcttgcct tttactacaa
      841 acttctgtca ggttgaatga tgaggcattt gcaagctctg ctatcgaagt gtgagagatg
      901 caattgcttg tgagattgca cagcaggtct gaatccaggc ttgtttcaat ctcaagtcct
      961 atggcttttt ttttgataag ctctgaaact gctaatccaa cctctaacct ttttttgctt
     1021 agctcctgct gcaaagcttc tttttctgcc aacgcagttc tttgtttaag catcaaagcc
     1081 tcgctgttaa aatcatctat aagttccctg atactctctg agagcgtaag atctgcaatt
     1141 ggctgccttg caatgttttc caaagcattc ctttcgttct cattctcatc gataatgcgg
     1201 ttgtaggact taaggagaga tgagaggtta ccctcagcct ccttgaacat atcatcagga
     1261 agatccaaat cctgcagaga ggcttcaagc gagatatagt cttcctcatc ccagatgcct
     1321 tggagataca acagcttttc tgcaagcaaa gaaatggact ggcttgtttc caggctcttg
     1381 tcttcaaaca ggacagtctt gtttagctca cctgccaaat caccaagggt tttttgcatg
     1441 ccattaagga tgcttgcttt gtgtgtcaaa gttttaatag agccggccag gttttccttt
     1501 agcctttttt cctctacagt cagatcatag tttactgtga ccaagatgct tcttgtcgaa
     1561 ggctctcctg ctgtgctgca gaacactgtc tttgcactgt aacagtctat gtcaaaacgg
     1621 tacaattcaa ggcctgtccc tatgcctgga gggattatag gataagcttt ttttagcgga
     1681 gaggaggacc ttaagataaa gctgtcctct tctatagtgg ttcctgcgct gatgtccttg
     1741 aaagagtatg tgcacaaagc cctgcaaaaa gggtttgtgg ccacttttgc ctggaactca
     1801 agctctgcat tttggccgtg gacaaggtag aggttccttt tgtttgcttc aagggtgact
     1861 acagtatcgt tgcctagaat aaatctaaag tatagccaga actttgctcc aaacaggatt
     1921 agtgccaaag ataggaggac tatggctgat atgataaaaa ccctatgttt tacgatatag
     1981 cggatggtct cttttaccgc ctgcttcttc ttagccatag gcaggattaa aaagtagata
     2041 ttaataaatt tagttgttta tgactaagtt aagtgcgcca tcatacgctg ctttttgagc
     2101 tttgtcttca gcaaattcat gcgtcatttg taaaatatct gctatctctt gcaaggtata
     2161 gcgaggtctt cttggaagga agagaggatt ttttacaaga cctgcttttt gtaatataat
     2221 tccacactgc tctggggaat aaaatactgg ctctcgccgt tgcctttcca agtgatattt
     2281 tacatggtaa gggatcactt tcctaccacc atgaatgccc atcgctgtcc atattttctc
     2341 catggtttca aaggagtcgg taacaatctg cggcaagtca tcagagtcgg tgcgtagtat
     2401 atcttcacct tgtactaata tcgctgaatt ccttctgtag ataacttgca acatggcaat
     2461 agcaggatct gcggtgtatt taacagttag ctcaatttca gcattatatg ctttagaaaa
     2521 aagaatatcc tttgctgcct gatgagcaac atgttcaagc ctagctattc tgcaaggtgc
     2581 cagtaactgc ataataaaaa atcccaaccg ctagccgagc tgcggggtat gaaccccagt
     2641 cttgagcaat caagaaatca agacagcgtt gacaacgcca tcctggcctg gccttgaggt
     2701 tatctttgct ttgccaagat ctgtctcgat gatagcccct tttgtcatga tatttcgcct
     2761 gacaaagtgc ctgttagctg gatttccgga aatggtcttt atcttccctt tttttgactt
     2821 gcctgtcttt tggtcaagga tatttgcaaa ttcttccgat agaagcctga gtttactgtt
     2881 tccgccaaga gtgtcgatga ctttaagcct ttttgcgcca agtcctgtaa atgcaggaag
     2941 ccttccaagc tcatattttc tataaaagcg gtaagccttt aatcttgagc cccttacgct
     3001 tcgtcttcct ctacccttga atacaaccat gaattctaag gattataggg gtatttataa
     3061 attttacgat atgagctccc tgagcgcttt ttagaaacat ttatatatga aataggatat
     3121 ctattgatat attatttagc ttttgagagg ggtgttttat ggaagcgtca agaccattgg
     3181 atgcattgaa cagagcaaga gacaagaaag ttatagttga actaaagaac cagaaacagt
     3241 atgttggaaa cttgaaatcc tttgacatac atatcaacgt tgtccttgag aatgcagaag
     3301 aaagaataga aggagaagtt aaaagaaaac tcggtgttgt atttatcaga ggagacacga
     3361 ttacagtaat atcaccggag taggcttatg acaaaaggaa caccctcaat gggtagaaag
     3421 tctggaagaa gaaatatgat ccattgccga agatgcggca agaggacata tcatataagc
     3481 aaaaagaaat gctcttcctg cggatacggc aggactgccc ggcttagaaa atataattgg
     3541 aacaagaaat aactcctttt tttctgatat tttttttaag tgctgtttgt tttattcaac
     3601 aaagatctga ccgctgcttg acatgcagct cttatctccc tataacttgc accttcacaa
     3661 atgcttatgt tcagctttgc ctgggctatc ttctgatagc agatgtcccc gacgacgact
     3721 gatctggtct cttcacacag gctttcctgg tttagagtta ttgcaacttt cctgatgcat
     3781 aggtcatgga ctgtatcgtt tctgataagg tcgcatcctt gccaatccaa ggtaactcgg
     3841 gagatggtat catagcactt gtctttttcc atcgtattgc taacattagc gcatggactt
     3901 gttttgtttg tcgatactgc gatgctgcta tagcatacat cagaccaata ctcggtatct
     3961 gtagatttgc acagttcaag gtctttttta ccaagcgcaa tctttgagat gcaataatcc
     4021 ttggatttcc cttcgatgct attacagatc gacaggtcgc tattcttgat gcctatttca
     4081 gccacgcatg agtcttttag aacttttgat tcgatggcat cgcaaccgtc aagggaaatc
     4141 tcacttacag ctttctcaaa atagcagtcg tccttggaag gacctttcag ggtgctgcat
     4201 gaagtgtctt ttgtgcagct tgctatcagt gttaatgctg ctataaccaa taatatcagc
     4261 catacttttt tcatcctagt gagcaagtta cgagagatat ttaaagctta ttaatcctgc
     4321 aaggtttaga aagcttttta taagaagtta aattttttta atttgcgagg atgccggagt
     4381 ggccaaacgg gtatggctta ggaccatata gcttagtgct tacgggggtt cgaatcctcc
     4441 tcctcgcatt ataaattcta ggagaaaggc taataatgaa atcgcacaca aaatacctac
     4501 agtttaacac caataacaag atagattttg tcaacattac ttcgcaagtt gaggatgcgg
     4561 tatctgaatc tggcgttaaa gaaggacttg ttcttgtaaa cgcaatgcat atcacttcct
     4621 cagtatatat caatgacgcg gaatctgggc ttatttctga ctttaaagaa tggctaaaga
     4681 agcttgcgcc gactgacatc aatctgtata agcataatct tacaggtgag gacaacggct
     4741 atgcccatct gtggagaacg ataatgggca gagaggttgt ttgtgctatc actggaggaa
     4801 agcttgattt tgggccttgg gagcagatat attatgctga gtttgatggg cagcggcaaa
     4861 aaagagtgct tgtcaagata attggggaat agttttccag gcgtagactt cagtatactg
     4921 gggtaatttt gtcattattt tgagtcgcaa actatataaa tcagcaaaat taagatagct
     4981 attgtgctgg gggtggtgga aaaccacaag gcacgaaagg ggggtgggtt tttttttacc
     5041 ttttttggta ttttcttgtt cacaatgcct aaaaaatgca tcatatgcgg cgaatgcgcc
     5101 gaattcaaca taaaaggctc aagcgaatgc tactgcaaag gctgtgcctc ggatagtttt
     5161 gaagaccttg gatatttaga agagctataa ttctcacttg ccacttgcca tgttgacgca
     5221 gagtatttaa gaaagaattt tttgaaaata aaaatcccaa ccgccccaag ggcggggtat
     5281 ttttagtgtt gtaaataccc tcaacgtggc gagccataac aataacccag tcaccgcaag
     5341 tgagtggggt atttacaaca aataaaaatg gctaagccaa tgacgctttc ccggtgtcac
     5401 atgggttcaa atgagtcaac catgagagtt tttgctgaaa ctcattgccg cacaaaatga
     5461 ttgaaaacag ttttatcttt cttgatggca taagagagaa gacagagcaa aatatatgga
     5521 accagggcat atataactgg gactctttta tccaggcaaa ggctgtgaaa ggcataggca
     5581 atacaagaaa atgctattat gacaggcaac tagagcaggc aaggaaggag ctgcatgatt
     5641 atggatttct gaataggttt cccttatgtg aaacatggag gatttatgac tatctaaagg
     5701 atgaatgcgt ttttctggat atcgagacat caggaatagg aaagcactgt gatataactg
     5761 ttgtcgggct ttttgatggg atagagacaa agagcatgat agcagggata aaccttgacc
     5821 ttagagcgct taaaagagag ctcgaaaggt ataagatgat agtaactttt aatggagctg
     5881 catttgatat cccattcatc aaaaaaagat acccttccct tctgccggag gttcctcatc
     5941 ttgaccttag atttgcttgt gaaaaggctg ggctaagtgg agggcttaag aagatcgaga
     6001 aagagctagg gatcgccaga aacaagctta tcgatgattt gtatggggga gatccatacc
     6061 tactatggag gatgtttagg ggctctggag atgagcatta tcttaatcta cttgttgaat
     6121 ataatgagga cgatgtgttt aatttgaaga agattgctga ctatgcttgt gataagctaa
     6181 aatctgatat tcttaagacg atgtacgagc cataatagaa agatatttat actaaattag
     6241 ctactttata gtaatgactg ataaacctac ggcaaggcaa gagttacata gaatcctaac
     6301 ccagatcgtt agaggagaat atccacggtt aggattcaac agcagcaaac caagaagttt
     6361 tgaaggcacc catattggag ttgccatgga ttcgattgac tttactgttt atcctaatga
     6421 cccatttaaa ttcaacggcg gacttattgc tcaggttgta caagtggtgg gtaaatatgg
     6481 ccaaagtggc cagtatgtca taaagtttta ttatttgcac tagctatctg ctccttccca
     6541 ctcctatctt agggcagtat ttccttaccg gacactttga gcaaaaaggg cttaccggaa
     6601 cacaaatatt ttgcccaaac ttaaccaaaa gctcgttgat atcgtaccaa tatttccttg
     6661 gaactatctt ctcaagctct ggctgggttt tttcagggga cgttgtgctt acccatccta
     6721 acctgtttgt tatctggaag acatggctgt ctactgctat atgatctggc ttgttaaagc
     6781 cgtagactat tgtgatggat gctgtctttg gacccacgcc tttaagattt atcagctctt
     6841 cgaaagtgcc tggcactgta ccttgatact tgcttatcag tgtctccgac agctctttta
     6901 tcctctttgc cttggtcttg taaaagccta ctggaaatat cagtttttca atctgttttg
     6961 cactaagctt tgcaatcttt tcaggggtgt tgtattttat cagaagtttt gttgatgctt
     7021 ttgcagtaac atcgtccttt gtccttaagc tcaggatgca tgagataagg gttgtaaagg
     7081 ggtttttttc gacccttgcc cagtggctta caaaaggatc tttccaattc actgactctt
     7141 tttttaggat agcgatgact ttggagatga ctgcccgttt tatttccttt tctgaattca
     7201 tcttgccaga tactgctttc ttttttcttc tggaaacttt tctattgcgt agcgaagcat
     7261 ggtccttggc atctgcctga aatatcgttt gagaaatatc tcttcctcag gtagtgacct
     7321 atttcctatc tcccttagca tccagcctac tgctttatgg ataaggtcat gatcatcatc
     7381 aagtaatatc tttgaaattt caagcgcgtc cctataatca ccttgcttta tgaaggcaaa
     7441 agtggccatg actgcaagtc ttctttccca cacagaacct gttcttgcca acctgtaaag
     7501 gatagttctt tttgtgtcca ggagaaagca gcctacaatg tttggcgctg acaaatccac
     7561 caagtcccaa ttgtttatgt tctttgtatt actcagataa aggtcgaata tctgctctct
     7621 ttctttctta cctgctttct taaacttgga aacaagtata aacaaagcag taagcctgtg
     7681 ctcatgtatc ctactcttta atagaggggt tatttcttcc aggctcagat gcagatattt
     7741 ctttgcaagc tttctttgtt ctgggacgat tattccaaag aagatgtctc cttctccata
     7801 ttctcctttg cctgtcttaa agaagcgccc taggatcttt gctttctttg gatttgcaag
     7861 cttttgcata tcatctttta tcatttgaaa acctttcctg tctggatgct ccagaacaac
     7921 aagtctaact catccatagg tatctttatc tgtcttgaga aattattaaa tttttcttct
     7981 agggccagat agttcttttt tgtcagcgaa gggatattat ctataacacc aagcgctagc
     8041 aagttcttta agatatgcct gtcaaggatg gcaaggccac tatagcctat gtttcttaag
     8101 aaatgcgatg cttccttata acccaggcct ttgacgtttt ttacaagcca ttctctgagt
     8161 tcttttggat cctttagctc atcaaggttg cgctcaatct ccttatactg ggctttgctt
     8221 aagtctagat aatgcgattt gttcttgtag aaccttgtcc ttggcctgag aaatggcttg
     8281 gtgtcaaatg agtttttatg gaatttcttg ttttttagca gttggatgca ttcatcgcat
     8341 tttagtgcgt ttgattgagg ggttagcagg caaaaacaga gctcatagaa gatatcgccg
     8401 ccctttactt tagaaaattc agaaagcctt tgcttgatta tatctttcct ttctttataa
     8461 gaagaatgta ataaataaat atccatctag ctgactgcag cgttcatctc tttgagcatc
     8521 ttttttatat cctcgtcgcc cattccatgc gcaagggatc cctgctctat ggtctcaaat
     8581 gcagcgatgt ggcagccgat gcaatgaagg ccatacttca tcaatatcgg ggctgcctta
     8641 ggatatttct caagaagctc tgagaactgc atctcttttg ttatcttttc tgctgccatc
     8701 tatgccaact tctttatttt aatagcaccg acagggcatg catcgactgc gtcctggttt
     8761 gttccaagct ctgcaacctg ggcttctttt acgacagcct tgcttccgtc tatctcaaaa
     8821 ttgtcacact gtgcagcgca tgcgccgcat cctatacact cgtcgctaac ttctatctta
     8881 tatgccattt taccaccatc ctaaaattct tcaaaatctt ctatctgctt cagatagcct
     8941 tttctcttta ggtattctac ctgagctttt gtgtatttgt agatgacatc tcccttatca
     9001 tcacctgaga attcccatgg ctcgacgatt attatgtcgc cctccctaac ccacaatctt
     9061 ctttttaagc ggccaggaat cctgcatata cgggtcttgc catcaaaaca tttaacttcc
     9121 atccttgagc cgccaagcct tctttggact actccaaaca cctgccttcc tcttggaagg
     9181 tggatccttg aaatctgttc ctgaagctct tcctggcgtg ctgcctcttc tttctttttg
     9241 ctcatttgta tttcgttata gtggtattgt ttataagtgt ttctttttta tttagggtaa
     9301 tcaatcgata atttggcttc tttttgggta taattcaaac catccaataa taatgggtga
     9361 aaacatgtac ttttcgctga aaagccctat ttgcagaacc acacttcgca tggttttttt
     9421 ctctgcactt ctgttttcac ccttattggt ggtgtgaaat cccaagcttt ttatataacg
     9481 atttcttttt tttatttatg gcaagaaaga aagggcttaa tcttcccaag gaaagaacgt
     9541 ttagcgggcc tgcatcgctt tggaaaagga tagctgcttt tatgactgac cttctgataa
     9601 tcgatatcgt catactgttc ccattgaatg gagttgtatc aaggctgata cccaagttta
     9661 gctctttttc agaggcagta gaagtgttct cctctggaag ctataactcg gggataatac
     9721 cccttgcttt tatgatggct attttatttt tcctctactt tacctggatg gaatacaaga
     9781 taaagcagac gcctggaaag atgctttttc gcatatcgat aaaaagcgac ctgcctgaca
     9841 tgaagctttg gcaggcggtt acacgaaata tatttgctgt acccatattt cctttcttcc
     9901 tgcttggaat aatggacttt gtattcctat tatttaacaa aacaggacaa aggttaagcg
     9961 agatattgag caagacaaga actgttgagg agtacccgtt agatggctga aagcaattgg
    10021 ggcactgcaa tcaagatcat ccttgtactt gtagtattat cctttatcgc tgctgctttc
    10081 ctgtcagtgt ctatcggaga agacaatgct gatggaaata tagctataat cccaataaag
    10141 ggtgtaatct ccactgaagg ttctggactt tttgaccagc aggttgcaag ttcagcagag
    10201 atagtgagtt tgatagagaa agcaagcaaa aatcccgaga taaaggctat catacttgag
    10261 ataaactccc ctgggggagc acctgttgca acagaagaga tagcaaacgc tatcgaatcc
    10321 acagacaaga tgactgtcgc atggataagg gagataggaa catctgctgg ctattgggtt
    10381 gcatcaagcg cagacaaggt tgttgccagc aggatgtcta caacaggctc aattggtgca
    10441 atcatgtctt atcttgactt ttcaggcctg cttgatgact ataatgtaac ctatcaaagg
    10501 cttgttgccg ggcaatacaa ggacataggg agccctttta ggcaccttac gataaacgag
    10561 cagcagataa tgcaaaagta cctcgatagg atgcatgagt atttcataaa ggccgttgcc
    10621 ataaacagga acatgactgt agagcaggta agggaagttg ccactggaat gtttttcctt
    10681 ggagaagagg caaaggagct tgggcttgtc gatgagcttg gcgggaaagc tgaggctgtc
    10741 aagataatag agaaccagct caatataact gcagtgaccg tgcagtacag ccacgagcca
    10801 acccttgggg agatacttgg cgacgtattt tcaaagcagt cattctatgt tggaaaaggg
    10861 ataggcagct cactccttga tgctggcagt tctaagataa gaatataggc atagaacaat
    10921 ttctgagtga tttttattgt gataaatgca aaaataacaa caacatagga tgagacggtt
    10981 attttgctct atgcaaaata acctacgaga catcccgacg ggacaacccc tgtctcgtct
    11041 catccaaggt tgttattttt gctaagcttg cagcactaaa ctcgtgcaag ctgattgcac
    11101 cctgtaacag catataggaa aatttttata ggagccttat cggattatta ttatgcctcc
    11161 tggtttaaat tatgcaggtg ttcttttact tgctctcatc atcactggct gccaggaagt
    11221 tgaatgcaat aaggattctg actgctttcc tgcagaatgc tgccatccga cttcctgcac
    11281 gaacataaag cctgattgcg acggcaggtt ttgctcgatg aattgtgcac ctggaaccat
    11341 ggattgcggc caaggatact gcgggtgtgt cgataataaa tgctctgctg tgatacaatg
    11401 aataagttcg cgatagtttt tttattgata gttgcaagca atattgtatt tgcaggaaag
    11461 atagatcctc ttgtcgagca ggagcttgag gaaaatggag aagcgtctgt cataatagtg
    11521 atgaatgagc ctgcaggcaa gaccttctta aatgcagccg gaaaagataa taggaagaga
    11581 gaaaatgtta tcgcttcact gtctgaagat tccaaattgt ctgtcaagca caaatactca
    11641 gccatcaatg ctttctctgc cagggtaagt gatagatcta tcgagaagct atcggatgat
    11701 ccaaacatca agagcgtgca tctaagcaag aggtattatg caacgctgga tgtaagcgtt
    11761 cctcttatca acgcaaccag cacatgggga atatctattg gcgaccaaaa cataacagga
    11821 gctgggcaga ctgtctgtgt catagacaca ggtgttgact ataaccacac ctcccttgga
    11881 agcggatggg gtaacgtggt cataggcgg
//
LOCUS       DASRUK010000130         2739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1105851, whole genome shotgun sequence.
ACCESSION   DASRUK010000130 DASRUK010000000
VERSION     DASRUK010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105851"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="VFF28_05885"
     CDS             <1..1068
                     /locus_tag="VFF28_05885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HZX45184.1"
                     /translation="ELWCGNSLCDGTEKKNSCPADCPSVCADNVCEGLENCYNCENDC
                     GKCEGDAFELIYDQNGNLIKDINYEYHFNSFNQLDSVSKRGRQIAQYSYDHNGKRIKK
                     VVFSGSANTTTYYLGNMVRVILGNSSIDTTYYSHDGQLVGSKSNGSTYYYHPDHLGST
                     NLITDDRGGFVSRSGYMPFGEQIIGMGDRYSFTGQEKDSETELMYYGARYYSPILGRF
                     TQPDTLLQNVYDPQLLNRYSYARNNPLKYTDPSGHAIPVLAFAALMIAIQVIVPAILG
                     YLAGYAVGATADIGKQLYEKRHAERLDIDFNEVNDAGNEIGGPLAASMGTQGLLEGLS
                     LNIGSFLPSTKPVPAKAQTDY"
     gene            1421..1984
                     /locus_tag="VFF28_05890"
     CDS             1421..1984
                     /locus_tag="VFF28_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45185.1"
                     /translation="MKGFEDKNKKLSVPWDLEFAKFKEIAVGLAYNDKYDQEWDNYNR
                     WQQEDIQNKGLKCITYQSEVARHKLTFTLFFVNNKLVKAEGSLKEQHLLNAFFDIYNE
                     ITSREGKPTTMKNFNANNASEILSQTEENFNPSLAWHGKDTTIQMEIEQKFAPTFRLL
                     FFKSGDETKLNEYPRLFNALERNHNGL"
     gene            2001..>2739
                     /locus_tag="VFF28_05895"
     CDS             2001..>2739
                     /locus_tag="VFF28_05895"
                     /inference="COORDINATES: protein motif:HMM:NF015497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HZX45186.1"
                     /translation="MIGILFSYTAFAIVGTGQTYKHYDNLISSAGFFSSDSYQTNIFF
                     GSAGYSYDIDIPQGIDSLQPSVSLSYSHHAAKMGVAGFGWGLSEAIIYRDTNFTRSNR
                     SDDGFRINLKGLDEELVYLSPRYHTLHESYMSIEKKTGSPNTFGEYWIARTKDGMKYR
                     FGYNNDSELVSNIETYAVSWRLDQVEDTHNNLITYSYKEEPSKAAAYLANITYGANSI
                     IFLYNFSAVQEIPVYSTGCEIRQTGLLK"
BASE COUNT          886 a          568 c          591 g          694 t
ORIGIN      
        1 gaactgtggt gcggcaacag cctatgtgac gggactgaaa aaaaaaacag ctgtcctgcg
       61 gattgcccga gcgtctgcgc agataatgtt tgcgaaggcc ttgagaactg ctataactgt
      121 gaaaatgact gtggaaaatg cgaaggagat gcctttgagc ttatctatga tcaaaacggc
      181 aacctgatca aggatatcaa ctatgaatat cattttaact cattcaatca acttgactca
      241 gtcagcaaaa ggggcaggca aatagcgcag tattcttacg atcacaacgg caagaggata
      301 aagaaagttg tgttttcagg aagcgccaat acaactacct attatctggg caatatggtc
      361 cgggtgatcc ttggcaacag cagcatcgac acaacctatt attcccatga tgggcagctt
      421 gttggaagca agagcaacgg tagcacatat tattaccatc ctgaccatct tggaagcacc
      481 aacttgatca cagatgatcg tggtgggttt gtgagcagaa gtggatatat gccttttggc
      541 gagcaaataa tcggaatggg tgataggtac tcatttacag ggcaggagaa ggacagcgaa
      601 actgagctta tgtattatgg agcaagatat tattcaccta ttcttgggag gtttacacag
      661 cctgatacat tgctgcagaa tgtttatgac ccgcaattgc tcaaccggta tagctatgcc
      721 aggaataatc cgctgaagta tactgatccc agcggtcatg ccatacctgt ccttgcattt
      781 gccgccctca tgatagctat ccaagtgata gtgcctgcta tcttagggta tctggcaggt
      841 tatgcagtcg gagcaactgc ggatataggc aaacaactct atgagaaacg gcatgcagaa
      901 agattagata tagactttaa tgaagtcaat gatgctggaa acgagatagg cgggcctctt
      961 gctgcttcta tgggaacaca ggggcttttg gaaggcctat ctttaaacat cggctccttt
     1021 cttccatcga ccaagccagt acctgctaaa gcgcaaaccg actactaggg tacccttgat
     1081 ggcccatcta tcccgtccga ggcatatcac catataggat ctaacagccc aagactacac
     1141 gcagtcatag aaaccaaagt tgtaacccct aacccttcaa ctggaaagag ttgggtaacc
     1201 tttgacaaaa tcgatgatgc atatagcgca ggtgaaatct tatcgacgcc agggaaggat
     1261 tcctttatca ggacggagct tgatacactt cagataatcg atgatctagc aatacctgct
     1321 gaaggcccgt ttacaaaggg tggagcatat caggccgtta ctggcagccc ttttacaata
     1381 agggatataa ctgatttgag gcagatacaa aggtgataat atgaaaggtt ttgaagataa
     1441 aaacaaaaaa ttatcagtac catgggacct ggagtttgca aaatttaaag aaattgcagt
     1501 gggacttgca tataatgata aatatgatca agaatgggac aattacaata gatggcaaca
     1561 ggaagatatc caaaataagg gattaaagtg tataacctat cagtcagaag tagcaagaca
     1621 taaacttaca ttcaccttgt tctttgttaa taataaacta gttaaggcag aaggatcact
     1681 caaggaacaa cacttgctca acgcattctt tgatatatac aacgaaatta catcaagaga
     1741 gggcaaacca accacgatga aaaacttcaa tgcgaacaat gctagtgaaa tactatctca
     1801 aactgaagaa aatttcaacc ctagtctagc ttggcatgga aaagatacaa ccatccaaat
     1861 ggagatagaa caaaaatttg cccctacctt ccgactactc ttctttaaat caggagacga
     1921 gacaaagcta aacgagtacc ctagattatt taacgcttta gagaggaatc ataatgggtt
     1981 atagatcaat attttttata atgatcggaa tcttattttc ttatacagcc tttgctatcg
     2041 tcggcactgg ccaaacttac aagcactatg acaaccttat ttcctctgcc ggcttttttt
     2101 caagcgacag ctatcagaca aacatattct ttggctctgc aggatactct tatgatatag
     2161 atatccctca agggatagac tcccttcaac catccgtcag cctcagctac agccatcacg
     2221 cagcaaagat gggtgttgct ggctttggat ggggactttc tgaggctatt atctaccgtg
     2281 acacaaactt taccaggagc aataggtcag atgacggatt taggataaac ctaaagggat
     2341 tagacgaaga gctcgtttat ttgagcccaa ggtatcatac gctacacgag agctatatgt
     2401 ctatagaaaa aaagacagga tctcctaaca cattcggaga atactggata gctcgaacaa
     2461 aggatggcat gaaatatcgt ttcgggtata acaatgactc agagcttgtc tcaaacattg
     2521 agacctatgc agtttcatgg aggcttgacc aggtagaaga tacccataat aacctcataa
     2581 cctattcata taaggaggag ccttcgaaag ccgctgcata tcttgcaaat ataacctatg
     2641 gcgctaatag catcatcttt ttgtataact ttagcgcagt ccaagaaatc cctgtctatt
     2701 cgaccggatg cgagataaga caaacaggac ttttgaaag
//
LOCUS       DASRUK010000131         3614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1106734, whole genome shotgun sequence.
ACCESSION   DASRUK010000131 DASRUK010000000
VERSION     DASRUK010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106734"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="VFF28_05900"
     CDS             <1..943
                     /locus_tag="VFF28_05900"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4070 domain-containing protein"
                     /protein_id="HZX45187.1"
                     /translation="NCEFCGIIAMNGREPRTKSSGQVIAELDSLYRAGWRGSVFFVDD
                     NFIGNRAKVNEVLKHIIEWQRNHRFPFVFMTEASLNLANDDEIMHLMSQANFDSVFIG
                     IETPCIESLKECGKHQNVNVDVAQAIKKIHKRGMQVLGGFILGFDSDNDSIFQTQIDF
                     IQKIGVAMAMVGLLNALPNTRLWYRLKEEGRLTGATSGENTDGSMNFIPRMGEDKLKK
                     GYRKVLNTIYCPKSYFQRVSKFLSDYKPTAKTEIKPAQVYAFVRSIWSIGILSRARFR
                     YWKILINTMLFNFRALPAAITLSIFWLHFSKVAHNIE"
     gene            988..3333
                     /gene="purL"
                     /locus_tag="VFF28_05905"
     CDS             988..3333
                     /gene="purL"
                     /locus_tag="VFF28_05905"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HZX45188.1"
                     /translation="MGMVDVETVPISDMSDSAISGFLKKNALALKVSECRKIIELIGR
                     DPTLTEIHMFNIEWSEHCSYKSSKETLKLLPTSGPTVILGPREDAGIIKFAEIDGEQY
                     GIVISHESHNHPSQVVPFEGAATGIGGNVRDVLCMGARVIASADPLRFGDPSGKKKNN
                     VKYVANAVVDGIAGYGNPLGVPVIAGDVYFNPSFDDNCLVNVVSLGIIKEKEVIHSAA
                     PPGSEGYDIVIVGKATDNSGFGGAAFASLILDEEEEEYNKGAVQVPDPFLKNILFRAT
                     EAVFAESRKRGVVLGFKDMGAGGIMCSTSELCDAGGYGGEIDIDKIHISMKNLPPYVI
                     ACGETQERFTWISPPEFTPTILKIYNDDFELPSVAEGACAKVIGKVTKSTDYIMRYEG
                     KVVCNVPVNVLTQGIRYQRERKELKRDFKEPILAEPRDYNAACLSVLSHPNVASKAKI
                     YKHYDTEVQGLAVIRPGEADAGMQAPIPGCDAGVALTVDHNPRYSRINPYLGGVNAVY
                     EAMRNIASVGATPYGMTDCLNFGNPEVPEVFNDFVESVKGIADAANKISLKVDGKSAV
                     PFVSGNVSFYNQSVSGKQVDPSPIISCVGFIEDYRKAVTMKLKEVGSELLLVGMRKDE
                     LGGSVYYELNNELGVNVPMVDFDKAKNQIYAVIDSIDDGAVLSCHDISDGGLLACISE
                     MVLGGEADGMIGADINIDSELRADKMLFSETPGFIIEAKADKVERLRNFFRKYNTNIV
                     RIGNTTNDKRLAIKLNKTQVVNLAIDDLKKAWTNGVVEALS"
     gene            3333..>3614
                     /locus_tag="VFF28_05910"
     CDS             3333..>3614
                     /locus_tag="VFF28_05910"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HZX45189.1"
                     /translation="MSKIAVVMFPGNNCENETARAVQAAGMESKVLRWNQTKELDSYD
                     GYIIPGGWSYEDRIRAGVIAAKDPVISMIKKEALKGKVVLGICNGCQILA"
BASE COUNT         1082 a          635 c          925 g          972 t
ORIGIN      
        1 taactgtgag ttctgcggga tcatcgccat gaacggcaga gagcccagga caaagagcag
       61 tggccaggtg attgctgaac ttgactcact ttatcgtgca ggatggagag ggtcggtttt
      121 ctttgtggat gataatttca taggcaacag ggctaaggta aatgaagttt tgaagcatat
      181 aatagaatgg caaagaaatc accggttccc ttttgtcttt atgaccgagg cgagcctgaa
      241 ccttgctaac gatgatgaaa taatgcatct tatgagccag gcaaatttcg acagcgtatt
      301 catcggcatt gaaacgccgt gcatagaaag cctaaaagag tgcggcaagc accagaatgt
      361 caatgtagat gttgcacagg cgataaaaaa gatacataaa agagggatgc aggtccttgg
      421 ggggtttata ttaggatttg acagcgacaa cgacagcata ttccagacgc agatagattt
      481 tatacagaag ataggtgttg ccatggcgat ggtcggcctg ctgaatgcac tgcctaacac
      541 taggttatgg taccgcttaa aagaagaggg aaggctgact ggagcaacgt ctggggaaaa
      601 taccgatgga agcatgaatt tcatcccaag gatgggtgaa gataaactaa aaaagggtta
      661 cagaaaagta ctgaatacaa tatattgccc taaatcatat ttccaaagag tgagcaaatt
      721 tttaagtgat tataagccta ctgccaaaac tgagataaag cctgcgcagg tttatgcgtt
      781 tgtaagaagc atctggagta taggaatatt gtcaagagca agatttaggt attggaaaat
      841 cctgataaat acgatgctgt ttaatttcag agcccttcct gctgctatca cgttgtctat
      901 cttctggctg catttttcca aagttgcgca taatatcgaa tagcataaga tttatataga
      961 aatcggaata ctttcttctc ggtcaaaatg gggatggttg atgtcgagac agtgccaatt
     1021 tctgatatgt ctgatagtgc tataagtggg ttcctcaaga aaaacgctct tgccctcaag
     1081 gtttctgaat gcaggaaaat catagaactt atcggcaggg acccgacact tactgagatt
     1141 catatgttca acatcgaatg gagcgagcat tgcagctata agtcaagcaa agagacactt
     1201 aagttgctgc cgacttctgg acctactgtc atccttggac caagagagga tgcagggata
     1261 attaagtttg ctgagattga tggggagcag tatggaatag tcatctcgca tgaatcccat
     1321 aaccatccct ctcaggttgt cccttttgaa ggggctgcga cagggatagg cggaaatgtt
     1381 agggatgttc tttgtatggg agcaagggtt attgcaagcg ctgatccgct tcgatttggc
     1441 gatccttctg gcaagaagaa aaataatgtc aaatatgtag caaatgctgt tgtcgatggc
     1501 atcgctggat atggcaatcc tttaggcgtg cctgtaattg caggagatgt ttattttaat
     1561 ccaagctttg atgacaactg ccttgtaaat gtggtgagtt taggaataat caaagagaaa
     1621 gaggtcatac acagcgctgc tcctcctggt tctgaagggt atgatattgt catcgtcggc
     1681 aaagcaactg ataattctgg ctttggcgga gctgcttttg caagccttat ccttgatgag
     1741 gaagaagagg aatacaataa aggggctgtg caggttcctg acccatttct aaagaatatc
     1801 ttgtttagag cgacagaagc tgtgtttgct gagtcgcgca agaggggagt tgtccttggc
     1861 tttaaggata tgggggctgg aggaataatg tgctcaacgt ctgagctatg cgatgctgga
     1921 ggatacggcg gtgagataga tatcgacaag atacacatat cgatgaaaaa tctgcctcct
     1981 tatgttatcg catgcggcga gacacaggaa agattcacat ggataagccc gcctgagttc
     2041 acaccaacta ttttaaaaat ttataatgat gattttgagc tgccatctgt cgctgaaggc
     2101 gcctgcgcta aagtaatcgg aaaagtaact aaatctactg attatataat gagatatgag
     2161 ggaaaggtag tctgcaatgt tccggttaat gtgcttaccc agggaatcag gtatcagcgg
     2221 gagagaaaag aacttaaaag agattttaag gagcctatac ttgcagagcc cagggattat
     2281 aacgcggctt gcctttctgt gctttcacat ccaaatgtcg cttcaaaggc aaaaatctac
     2341 aaacactatg atactgaagt tcaagggctt gctgtgatta gaccagggga agctgatgct
     2401 ggaatgcaag cgcctatccc tggctgcgat gctggtgttg ccttgactgt cgaccataat
     2461 ccaagatatt cacggataaa cccttatctt ggcggcgtca atgctgtcta tgaagctatg
     2521 aggaatatcg cttctgtcgg tgcaactcct tatgggatga ctgactgcct taatttcggc
     2581 aatccagaag tgcctgaggt cttcaatgac tttgttgaaa gcgtcaaggg aatcgctgat
     2641 gctgcaaaca agatatcatt aaaagttgat ggcaagagcg cggttccatt tgtttctgga
     2701 aatgtatcat tttataatca aagcgtgtct ggaaaacagg ttgatccttc tcctataatc
     2761 tcctgcgtag ggtttattga ggactatagg aaggctgtta cgatgaagct aaaagaggtt
     2821 ggttctgagc tgctgcttgt aggcatgcgc aaagatgagc ttggtggaag cgtttactat
     2881 gagctcaata atgaactagg agtaaatgtt cctatggttg attttgacaa ggcaaagaac
     2941 cagatatatg cagtcattga cagcattgat gatggagcag tcttatcgtg ccatgatata
     3001 tccgatggag ggcttttagc atgcatatcc gaaatggttc ttggcggaga ggctgatggt
     3061 atgatcggcg ctgatataaa tattgattcc gagctgaggg ctgataagat gcttttctct
     3121 gagactcctg gatttatcat tgaagctaaa gctgataagg ttgagagact cagaaatttt
     3181 tttagaaaat ataacactaa catagtcagg ataggaaata cgacaaatga taaaaggctt
     3241 gcaataaagc tgaataaaac tcaggtggta aatctggcaa ttgatgactt aaaaaaagcc
     3301 tggacaaatg gcgttgttga ggcgctttca taatgtcaaa gatagctgtt gtgatgtttc
     3361 ctggaaataa ttgcgagaac gagacagcaa gagctgtgca agctgctggg atggaaagca
     3421 aggtgttaag atggaatcaa acaaaagagc ttgattccta tgatggttat atcatccctg
     3481 gcggatggag ctatgaagac aggataagag ctggagtcat cgctgcaaaa gacccggtga
     3541 tttctatgat aaagaaagag gctttgaaag gaaaagttgt gcttggaata tgcaatggct
     3601 gccagatact tgct
//
LOCUS       DASRUK010000132         4490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2123059, whole genome shotgun sequence.
ACCESSION   DASRUK010000132 DASRUK010000000
VERSION     DASRUK010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2123059"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..567
                     /locus_tag="VFF28_05915"
     CDS             157..567
                     /locus_tag="VFF28_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45190.1"
                     /translation="MIGREEIFTNGAGIVSITIYKNEGIGACAFSGQYEEVFCSYRLA
                     LEINNASAEKQAQITSSLGTINESGISYLSENLTLYLDGKKLDDIRISSELKGTVNNY
                     IQISGFGSGKDRKKAIDDAKKKHEALANIFLDIK"
     gene            658..1119
                     /locus_tag="VFF28_05920"
     CDS             658..1119
                     /locus_tag="VFF28_05920"
                     /inference="COORDINATES: protein motif:HMM:NF016363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HZX45191.1"
                     /translation="MSFFSKIKEAISGDTSEILKETEEEGYVELGANPGSKESKIIVR
                     PYILQDFEAIKPILEALREGHTVALVNIKPLKDKDLVELKRAVNKLKKTCDAIEGDIA
                     GFGDDWIAAVPSFAKIHRAKGPEAKESKKVEEVTEYSSEPQGDEDPKIETY"
     gene            complement(1116..1286)
                     /locus_tag="VFF28_05925"
     CDS             complement(1116..1286)
                     /locus_tag="VFF28_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45192.1"
                     /translation="MAVFLISSAIVLALGFILFIFGLIKYVRRISKWPLIASVLLFIL
                     GFILALLGLMFD"
     gene            complement(1328..1642)
                     /locus_tag="VFF28_05930"
     CDS             complement(1328..1642)
                     /locus_tag="VFF28_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45193.1"
                     /translation="MATSYTCSVCKRKFAFEQIRYSQNGSHIVCKGCYVSCVEEKPSA
                     VRIEVPIMEPTENKFRCLGCGYRFSIKKESMMARSCPYCGKASSLISDNISAQSIINS
                     VR"
     gene            complement(1652..1978)
                     /locus_tag="VFF28_05935"
     CDS             complement(1652..1978)
                     /locus_tag="VFF28_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45194.1"
                     /translation="METGMGDYFIETKVKLLVDMQLKKMAGDMQKMAETIAALNQEIL
                     MLKSQVNERRDAPKPIFVAPEPMAAPAASVVSSAPKNGEQLRPRYGDYKPEDVSIGKF
                     FYYGGK"
     gene            complement(1984..2715)
                     /locus_tag="VFF28_05940"
     CDS             complement(1984..2715)
                     /locus_tag="VFF28_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45195.1"
                     /translation="MEEETIVKRMVAGIVESNEKVFSQPQDIGKFSRIVPSLIKKGLD
                     NLNLNMFSPELRTQIITALGEEYKRKGNLNDAVRAFVLAGNRQKLNEVASDYERLSQF
                     DNCIEVYKLANNKDKLMEIGKKCLNESRLAHAMKAFIAIGDTSELIEVGNQCVNKCRF
                     DYAFEIFSTIKDKDKLVELGKKCLEEKSYDHAIKAFEVADYKEGLNLAGDICMKDESI
                     SKALEAYQLAGNEEMAQFIKENFKE"
     gene            complement(2726..3784)
                     /gene="ftsZ"
                     /locus_tag="VFF28_05945"
     CDS             complement(2726..3784)
                     /gene="ftsZ"
                     /locus_tag="VFF28_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HZX45196.1"
                     /translation="MAFDSMFTNAVKEEDMQIGHANIKVIGVGGAGSNMVSWLYKKGI
                     KGAHIIACNTDQQHLNLTEADYKFLIGRQITRGLGAGGFPQRGAQAAQEAMNEIKECL
                     KNTDMVFVCAGMGGGTGTGAAPFVANIARETGAIVIGTVTMPFKIERARIDRAEFGLQ
                     QLREVSDTVIVIDNNRLVSIAGNLPVQQAFAVANELISTMIRGIVETISVPSLVNLDF
                     ADVKAIMKGGGVAAIGVGVSDTASRVEEAAKGALSNPLLDIDYSGAEGALIHIEGGPD
                     MTLDEVEKIGELVTAKMDENANVIWGARVNEQMKGKVCVMTIITGVNSPWILGKNDAK
                     AAKFQRSKMSQELGIDVI"
BASE COUNT         1241 a          997 c          840 g         1412 t
ORIGIN      
        1 aagcagttta ttgaggtggt ttacaacaag aagaggctgc attcatccat aggatacgtg
       61 ccgccagatg agtttgaaaa cgaggcttta aatataagtt tagttcgtta actgactgtc
      121 cagaaaaagg ggcgcagtcc agaaacattt aaagcgatga ttggcagaga agagattttt
      181 acgaatggtg caggtattgt cagcataact atctacaaaa atgagggtat aggcgcatgc
      241 gcattttctg ggcagtatga agaggttttc tgcagttacc gcttagcgct tgagataaat
      301 aatgcctcag cggaaaaaca agcgcaaatt acctcgagtt taggcaccat aaatgaaagc
      361 ggcatcagtt atctctcgga aaatttaaca ttatatttag acggaaaaaa acttgatgat
      421 ataaggatca gctctgagtt gaagggcact gtcaacaatt atatacagat atcgggcttt
      481 ggaagcggaa aagacagaaa gaaagcgata gatgatgcaa aaaagaagca tgaagctctt
      541 gcaaacattt ttttagacat aaagtgatca tccctttctc cacgagagac cgcaaagttt
      601 atatatccgt tggctaatca acattggagc atagtaacta ttaagggagg ctttaaaatg
      661 agcttttttt caaaaattaa ggaggcgatt tcaggagata cttctgaaat acttaaagag
      721 acagaagaag aaggttatgt tgagctaggt gcaaacccgg gttcaaaaga atcaaagatc
      781 atagtcagac catacatact tcaagatttc gaagcgatca aaccaattct agaagcatta
      841 agggaaggtc atactgtcgc tttggtcaac atcaagccgc tcaaggacaa agacttggta
      901 gagctaaaaa gagcagtaaa caagcttaag aaaacctgcg acgccattga aggagatatt
      961 gcaggatttg gtgatgactg gattgccgct gtgccatcat ttgcaaagat ccatagggca
     1021 aaagggccag aggcaaaaga gagtaagaag gttgaagaag tcactgaata cagttcagag
     1081 ccgcaaggcg acgaagatcc aaagatagag acttattaat caaacataag gcccagcaat
     1141 gccaagataa atcccaggat aaatagtaag acagatgcga tcaagggcca ttttgatatt
     1201 cttctaacat attttattag tccaaatata aataatataa atccgagtgc aagcactatc
     1261 gctgatgata tcaagaaaac tgccattaaa aacaataaga cttaaaagaa taaaaagctt
     1321 tcgaaaatta tctcacgctg ttgattatgg actgagcgct tatattatcg cttatcaggc
     1381 tgcttgcctt cccgcagtat gggcagcttc ttgccatcat tgattctttc tttatggaaa
     1441 atctataacc gcatccaagg catctaaact tgttctcagt cggttccatg ataggcacct
     1501 ctatcctgac agcagatggt ttttcttcta cgcatgatac atagcatcct ttacacacta
     1561 tgtggctgcc attctggcta tatctgatct gctcgaacgc aaatttcctt ttgcacacac
     1621 tgcatgtata agacgttgcc atttttctca cctattttcc gccgtaataa aagaacttac
     1681 cgatgcttac atcttcgggt ttgtaatcac catatctggg ccttagctgc tctccatttt
     1741 ttggcgctga agaaacgaca cttgcagcag gcgccgccat cggttcagga gcaacaaata
     1801 ttggttttgg agcatccctc ctctcattaa cttggctctt cagcatcaat atctcctggt
     1861 taagtgcagc gatggtttca gccatcttct gcatatcgcc agccatcttt tttagctgca
     1921 tgtcaacaag cagcttcact ttggtttcga taaagtaatc tcccattcca gtttccattt
     1981 tcctcattcc ttaaagtttt ccttgatgaa ctgggccatt tcctcatttc cagcaagttg
     2041 gtatgcttcc aaagccttgg agattgactc atctttcatg caaatatctc ctgccagatt
     2101 aagcccttcc ttataatctg caacctcaaa tgccttgata gcatgatcat agctcttttc
     2161 ttcaaggcac ttttttccca gctctaccaa tttatccttg tcttttatgg ttgaaaagat
     2221 ctcgaatgca taatcaaatc ggcatttgtt tacacactgg tttccaacct cgataagctc
     2281 tgatgtgtcg ccgattgcga taaaggcttt cattgcgtgt gcaagcctgc tttcgttaag
     2341 acatttcttt cctatctcca tcaatttgtc tttgttgttt gcaagcttat atacctctat
     2401 gcaattgtcg aactgagata atcgctcata gtctgaagcg acttcgttga gtttttggcg
     2461 gtttcctgca aggacaaacg cccttactgc atcgtttagg ttaccttttc ttttgtattc
     2521 ctcacccagt gctgtgatta tctgtgttct aagctcaggg ctgaacatgt ttaagttcag
     2581 gttatccagg ccttttttga taagggatgg gactatcctg ctgaattttc cgatgtcttg
     2641 tggctggctg aatacttttt cattgctttc aacaatgcca gcaaccattc ttttgacgat
     2701 ggtctcctct tccatttttc cctccctaga ttacatctat gccaagctct tggctcatct
     2761 tgcttctttg aaacttagct gcctttgcat cattctttcc tagtatccag ggcgaattga
     2821 ctcctgttat tattgtcatg acgcagacct ttcctttcat ctgctcattg actcttgcac
     2881 cccagatgac gtttgcattc tcatccatct tagccgtaac aagctctcct atcttctcaa
     2941 cttcatcaag ggtcatatca ggtccaccct ctatgtggat aagtgcgcct tctgcacctg
     3001 aatagtcaat gtcaagcaga ggatttgata aagcgccttt tgcagcctct tcaaccctgc
     3061 ttgctgtatc acttacgcca actccgattg cagctacacc gccgcctttc atgatcgctt
     3121 taacatcagc aaaatccaag ttgacaagcg agggtactga gatagtctca acaattcccc
     3181 tgatcatcgt cgatatgagc tcgtttgcga cagcaaaagc ctgctgcaca ggaaggtttc
     3241 ctgcgatgct tacaagcctg ttgttatcta tcacgataac tgtatcgctg acttctctta
     3301 gctgctgtag cccaaactca gccctgtcta tccttgctct ttctatcttg aaaggcatag
     3361 ttacagtgcc aatgactatt gcaccggtct ctcttgcaat gtttgcaacg aatggtgcag
     3421 cgcctgttcc tgttccgccg cccattccag cacatacaaa taccatgtct gtgtttttta
     3481 agcattcttt tatctcattc atagcctctt gagcagcctg tgctcctctc tggggaaaac
     3541 cgccggctcc aaggcctctt gtgatctgtc ttccgataag aaacttataa tcagcttcag
     3601 taagattaag atgctgctgg tcagtattac atgcgattat atgagcaccc tttatgccct
     3661 tcttgtagag ccaggatacc atattgcttc ctgctcctcc aacgcctatt acctttatat
     3721 ttgcatgacc tatctgcatg tcctcttcct tcaccgcatt cgtaaacatt gaatcaaatg
     3781 ccattttttc accccccttt ttccgggatt tttttgtgga tttgtctgaa atcgctttct
     3841 atcaatatct ttttgatatc aacccctttg aacatgccag cgatttctga gcatgctcaa
     3901 gaacctgggg gttcttgaca ttgtgagaga gcgtttttcc gctttcttat ctttttaagg
     3961 gttatactaa tatataaatg ttactatttc gtatactggt ttaggacttt cacgtaactc
     4021 tatcgacgat aaactgattt tattgccatt ttggagaggg tcaaaaaata cccttttttc
     4081 ccctttgcga ttagaaaata actggcggat aagcctctat tttaattgca aagcaaaaaa
     4141 ccgctcattt gtttaacaaa agctcatttt tgaccctgta agcctaaata agaaagaatc
     4201 tctccaatta tcttattggc aagcttttgg ttcaatctca gtaaagggtg tactctgttt
     4261 tttcctccaa gcgattaaac ctatcgcatc atttatatta tgtatcatgc ttctaatgaa
     4321 tacataaaat gagtaaaaga cgacaaactt caccttgcta ttccaatgct aattttattt
     4381 gcatttatac gacttcttct aaaattgttt tgtaataatc gtagtaaact tgatcttata
     4441 ttgacaaact gacggatttc tgtatacagt tatatctttc tgtatagtaa
//
LOCUS       DASRUK010000133        10886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1291864, whole genome shotgun sequence.
ACCESSION   DASRUK010000133 DASRUK010000000
VERSION     DASRUK010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 10886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10886
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291864"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..793)
                     /locus_tag="VFF28_05950"
     CDS             complement(53..793)
                     /locus_tag="VFF28_05950"
                     /inference="COORDINATES: protein motif:HMM:NF025864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmeII family type II restriction endonuclease"
                     /protein_id="HZX45197.1"
                     /translation="MKGIPIREAREYIHDNIGQFHEKRLRSLQETKFYDLLKRKNPYL
                     FRAKYILTAESLVTSLEAKLSSSEEEIFGEFLEDLAIFVAQKTLGAVKSSTEGLDFEF
                     TRKNVRYLFAIKSGLNWGNSSQWKALIVSFKRAKQVLNQSRHIKEVKCFLGISYGKVK
                     RTEKKGFILQVCGQEFWAVLSGDPKFYSELIEPIGYKSKELNENFKTEKAKIINKFTR
                     DFLNEFCDESGKILWKKLVKFNSGNMEL"
     gene            complement(771..1637)
                     /locus_tag="VFF28_05955"
     CDS             complement(771..1637)
                     /locus_tag="VFF28_05955"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HZX45198.1"
                     /translation="MTQKVLIERDEECLPTFRNQCLVGDCLNILKTIPPNTYDLIVTS
                     PPYSDQRKNSYGGVHPDNYVSWFLPRSLEFLRVLKPTGTFILNIKEKVVDGERHTYVL
                     ELIQKMREQGWLWTEEFIWHKKNTYPGKWPNRFRDSWERCLQFNKQRNFHMYQEAVMV
                     PVGDWAQARLTSLSATDKKRAASKVGSGFGKNISNWVGRSWVYPSNVLHIATECGNKN
                     HSAVYPTELPEWFIRLFTKKGDIVLDPFAGSGTTAVVAKRLGREYTLIDILKTFKNIA
                     EERIRNEGHSDS"
     gene            1908..2987
                     /locus_tag="VFF28_05960"
     CDS             1908..2987
                     /locus_tag="VFF28_05960"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006330.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HZX45199.1"
                     /translation="MDIDKRIEVIKQVGEEIITEEDLRTLLSEKKNPIAYDGFEPSGT
                     DIHIAQGLLRAININKMTAAGCKFKILVADWHAWANNKLGGDLEKIQKAGQFYIKIWK
                     ACGLDEKNIEFVWANDIIKNPSYWKIVMQVSRNSTLKRIIRCGQIMGRKEDEVMQASQ
                     ILYPCMQCADIFYLGVDICSLGQDQRKVNVLAMELAQRLGFKKPVAVHHHMLMGLGQP
                     PKTGLSGVERGVELKMSKSKPDTAIFMLDSEQEIARKIRKAWCPEGIVDQNPILEYFK
                     YIIFEKFDLIIIERPEKYGGSLEFSSYKDLEEGYSKKNIHPMDLKETVSKYLNKLIAP
                     VREAIFSNPESKKLYEEVQGFKVTR"
     gene            complement(2988..4193)
                     /locus_tag="VFF28_05965"
     CDS             complement(2988..4193)
                     /locus_tag="VFF28_05965"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZX45200.1"
                     /translation="MEKFILDNGLTVISQKKPIDTVTVQCTVKVGSNYETKKNNGISH
                     FMEHMLFEGTKRRPDARAISNEIESIGGDLNAYTSSERTCFFIRVPKEHYNLALDIMA
                     DMLQNSVFDKEKLEKERKVILKEVNLHKDDPRFHQWILFSKALFGDYPAGLPTYGLVG
                     SVKKITRTDLQNFFIKFYQPSNTVLSIVGNIPNDKNLIRSIFSKKANINQIIPKTISI
                     ASEKKNIIKEKRKVLSSYYVLGYKTAPRKDEDSYVLDVIRSILGRGQSGRLFDEIRNK
                     HGLAYEVGVHHDPLVDYGVFAVYFNTDKENLKKITELIRTQFDVLKGLSDKELNEAKN
                     SLIGQHIMQNDDPKELADSLGFWELIKDAALLDRYLDRVKKVTKKDVARVAHKYLTEN
                     YSLSIIEQR"
     gene            4233..5315
                     /locus_tag="VFF28_05970"
     CDS             4233..5315
                     /locus_tag="VFF28_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HZX45201.1"
                     /translation="MARIKEFQEIMRKKKVDIFLAMNTDFSRLEYDMAYFTGYGSIGA
                     LIIPQQQKPFLIVPRMEKERAKRSGVLVYSPPKGMRISAYANEILKKKGVRTKKAGIN
                     KEEMTIVIRDSFRKALKARFVDLRKDIYKLREQKTADEIKTIREGCKISDSILEKCFR
                     EFKNFQTEAAVKAFLEIEAKKRGCEIAFPTIVASGPNARMAHYDICDKKLMNGFCIID
                     YGIRYKNYCTDTTRTVYIGEPSKKEIELYNLVLSCQRKAIESSKIGAKCSKIYEGAVA
                     DLGKYGKYFTHGLGHGFGIKVHEAPDLTPKSKYTLKEYMTFTIEPGVYLDNLGIRIED
                     DILITDRTEILTRAKKELVVITRGRT"
     gene            5312..7084
                     /locus_tag="VFF28_05975"
     CDS             5312..7084
                     /locus_tag="VFF28_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45202.1"
                     /translation="MKRYNLKELEAASLRYKRALGNIKGKTAFIVLDIRQKDAFYSLA
                     PLSKAMAELGCDLNVQAINKKSESINALADVWKCYKDLSGGVKNARTKALKDFLYAAD
                     KKCKGKLNGLFREPEVRLNATAGGFNGSYFLKYETDWMNEYRKKDLVQTAKVICKQVY
                     NLKKNEKISIGFDILRKKEDLGLPIEDYLDSFQISYAMMEAVKNQVTGMGAYTSRRSQ
                     MDEPERIADLKSTLLGCELCKDVDEAIFKKYKVLSKALRLDRIKVVSSASFSIHGKGY
                     AGRHYFGEYIGYPSKNKKTRWLSPGQMIYKLDYYPQTALDPRAPMTRIGFTETLPIDI
                     FIKTSNIDWLAMKRRDDKIIDITNKCEKIIVEGKKIGKYQTKLEIGLVNKNKRRKFRG
                     SDVETRNLINPWYYKETGIKAGTMANIPGGEAFVTPEYVKGQFIGDVVISLDQSYVLS
                     GKEPMVVSCFGNSYKAVSGPQKILEKFKQKKDEAWKKLKMQEKNKSLPKQVLEVNKRN
                     FNNIGEFAINTNPQAELCNYLIVNEKIANMIHLALGSGFEADRATYYHTDIVIDSPRQ
                     QLDIYGVAKDGKKHWIHRKGKFVV"
     gene            7038..8006
                     /locus_tag="VFF28_05980"
     CDS             7038..8006
                     /locus_tag="VFF28_05980"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HZX45203.1"
                     /translation="MAKSTGYTGKESLLSNLREVYRVNLAVKKNERVLVVIDRRKRKL
                     GRLFLAAGKDFSPCIDLMEIPVGKVNGEEPPSAVAKQMQKHDVLILLTTKSLTHTNAR
                     KNACRKGARVASLPDATEDMLKRTLSVDYRLMHKRIAEISDVLDKGKIVRIVTPAGTD
                     LLFSIKGRRAYGRESGIFTRKGRYGNLPDGESFIAPVEGSASGVFIIDGSIGSMGMVD
                     RPVKVFVQKGSVLKIAGGKAAKVLMKQLNDAGNKAKNIAEFGIGMNDSAKITGNMLED
                     EKVIGTCHIALGNNSGFGGKVDVPLHIDCLIKKPVIYIDEKLLRIP"
     gene            8040..8819
                     /locus_tag="VFF28_05985"
     CDS             8040..8819
                     /locus_tag="VFF28_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45204.1"
                     /translation="MAFNDRLRISWAEYKGGLKAYLAITALFIIIPDLLRYLVAIPLG
                     LEYYASGADIFKLGSTIFEPKFLIPNMTMLILNFVSRTFAVASIIYFCVYRRKSFIEA
                     LYGGRRYLLKCIWLSLVLGFFVSGLLFLLVIPGIIFSIYWSFSYFVLIGTNKGVIGSL
                     KESREIIKGNWWKVFGYLICFALFVSILALPFKIIGDVVIKTFIGLSTPITKDILSKI
                     FEWIPQLITIPLIVLFLKNLFLELRREKLMIYQSIPTKNWH"
     gene            8882..10033
                     /locus_tag="VFF28_05990"
     CDS             8882..10033
                     /locus_tag="VFF28_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011153181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZX45205.1"
                     /translation="MPPSDSSRNILDSVIYRYTTNSSQLEEDNKILKETITKLQKEVE
                     KFKLPPLMVCEVRELEDETAIISIPNGNQFSVCISDTCDKIRPGDMVLAEQKNLVIIK
                     KIPIARRFNVEKFVIVEKPTVSWKEIGGLDKQINEIKEVVELPLKKPELFKKVGIKPP
                     KGILLYGPPGTGKTLLAKGVAAATDSTFIEIVASELVQKFIGEGAKLVKEIFEMARHK
                     APAIVFIDELDSLAAKRVEIGTSGEREVNRTFMQLLAELDGFDPLDNVKVIGATNRKD
                     ILDPAIIRPGRLDRLINVGIPEKEAKLQILKIHSKNMTLEKGINFNTLVDSMEEFSGA
                     EIRAVCTEAGYFAIRNNRTSIIREDFSKAIEKVKKDENAEGDDYLRMFG"
     gene            complement(10016..10540)
                     /locus_tag="VFF28_05995"
     CDS             complement(10016..10540)
                     /locus_tag="VFF28_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZX45206.1"
                     /translation="MQELFDIVDDYNNIIGTATRKEAHEKGFLHKSGIFFIFNKKGQL
                     FMNRRSLKKEFEPGVFSLVLGGHVIKGDSYEQTVIREAKEEAGITSKPHYLANFKSRF
                     KEKDKENAALYYFIIDHEPILDKTEIESGRFIDIDEIQRMVKIGHFIRETQVIYPLLL
                     ENKEKILKALAKHP"
     gene            10677..10865
                     /locus_tag="VFF28_06000"
     CDS             10677..10865
                     /locus_tag="VFF28_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HZX45207.1"
                     /translation="MAEYIELNAFIKMKGFAATGGQAKVLIRSGAVKVNGQVDTRNKK
                     KLTSTPTLKGGDSVELLA"
BASE COUNT         3382 a         1954 c         2369 g         3181 t
ORIGIN      
        1 agggttagaa agttgattag aataccccaa tatataagaa aaaggcaatt agttagagtt
       61 ccatattacc tgaattaaac tttactaact ttttccatag tattttccca gattcatcac
      121 aaaattcatt caaaaaatcc ctggtgaact tattgattat ttttgctttt tctgttttga
      181 aattttcatt tagttccttt gatttatacc ctatgggttc tatcagttct gaataaaatt
      241 tggggtcgcc actcagcacg gcccaaaatt cctgcccaca tacctgaagt atgaaacctt
      301 tcttttctgt tctttttact tttccatatg aaatacccag gaagcatttt acctctttga
      361 tgtgccttga ctggttcaaa acctgctttg ctcttttaaa tgagacaatt aatgccttcc
      421 attggctgct attgccccag tttaggcctg atttaattgc aaataaatac cttacattct
      481 tacgggtgaa ttcaaaatcc aatccttctg ttgaagattt tactgcccct aatgtttttt
      541 gagcaacaaa aattgccaaa tcctccaaga attcaccgaa gatttcctcc tcggatgagg
      601 aaagttttgc ttccaaagaa gttactaaac tttctgcggt gagaatatat ttagccctga
      661 acaaataagg atttttcctt tttagcaaat cataaaactt tgtttcctgc aatgatctta
      721 atcttttctc atgaaattgc cctatattgt catggatata ctctcttgct tcacgaatcg
      781 gaatgccctt cattccttat cctttcctcg gcaatgtttt taaatgtttt taaaatatca
      841 attaaggtat attctctacc taaccgtttt gctactacgg cagttgttcc gcttcctgca
      901 aatggatcaa gcacaatatc tcctttttta gtgaagagtc tgataaacca ctcgggcagc
      961 tctgtagggt atactgcact gtgattttta tttccgcact cggttgctat atgcaaaaca
     1021 tttgagggat atacccaact tcttcccacc caattggata tatttttacc gaaaccactt
     1081 ccaaccttgg aagcagccct ttttttgtca gtggcactta aacttgttaa ccttgcctga
     1141 gcccaatctc ccactggaac catgactgct tcttggtaca tatgaaagtt tctttgcttg
     1201 ttgaattgca gacatctttc ccaagaatct ctaaacctat tcggccattt gcccggatag
     1261 gtattctttt tatgccatat aaactcttct gtccataacc atccttgttc cctcattttc
     1321 tgaataagtt caaggacata tgtatgcctt tctccatcaa cgactttctc ttttatattt
     1381 agaatgaacg tcccagttgg ttttagtacc ctaagaaatt caagtgatct tggtagaaac
     1441 cagctaacat aattatccgg atgaacccct ccgtaactgt tttttctttg atctgaatat
     1501 gggggcgaag ttacaataag gtcgtaagtg tttgggggga tagttttaag aatgttcagg
     1561 caatcaccaa caagacactg gtttctaaaa gtcggtaagc attcttcatc tctttcgatt
     1621 aacacttttt gagtcatttt ttaccttgtc gattagattt tcggggttta ttccaatagg
     1681 ggctcttgca ttttgggcaa attattggat tgaaccttat ttttgtcctg ggtatccact
     1741 catgcccaca tctctcgcat tcatatccct ctatagtaat ttttatttta cccattatcg
     1801 attaataatg aagtccatat taataaatct tactccttag gtataaccta ttaaggtaac
     1861 taatttcccc aaacctttaa ataactcatt attatttact ggttcttatg gatatcgaca
     1921 aacgaataga ggttatcaaa caagtcggcg aagagataat cactgaggaa gacctaagaa
     1981 ccttactttc tgaaaagaaa aatcctatcg cttatgacgg ctttgagcca tctgggactg
     2041 atatccatat tgcacaggga cttcttagag cgataaacat aaacaagatg actgcggctg
     2101 gctgcaagtt caagatcctt gtggctgact ggcatgcatg ggctaacaac aaattaggag
     2161 gggatttgga aaagatacag aaagcaggcc aattttatat aaaaatatgg aaagcctgcg
     2221 ggcttgatga aaaaaatatt gagtttgtat gggctaatga cattatcaaa aatccatctt
     2281 attggaaaat agttatgcag gtttctagaa actcaacgct caaaagaata atccggtgcg
     2341 gtcagataat gggaagaaaa gaggatgagg taatgcaggc atcgcagata ctttatccat
     2401 gcatgcaatg tgcagacata ttttatcttg gtgtggatat ttgttccctt ggccaagacc
     2461 aaagaaaggt taatgttttg gcaatggaat tggcacagag gctgggattt aagaaacctg
     2521 ttgctgtcca tcaccatatg cttatggggc ttgggcaacc tccaaaaacc ggactttcgg
     2581 gggtcgagag aggagtagaa cttaagatgt caaagtcaaa gcctgatact gctatattca
     2641 tgcttgattc tgaacaagag atagcaagaa aaataagaaa ggcatggtgt ccagagggca
     2701 tagttgatca aaatcccata ttggaatatt ttaaatatat aatatttgag aagtttgact
     2761 taattattat tgaacggccg gaaaagtatg gaggcagtct tgaatttagc tcatataaag
     2821 atttagagga aggatattca aaaaagaaca ttcatccgat ggacctaaaa gagacagttt
     2881 caaaatactt gaacaaattg atagctccag taagagaagc aatctttagt aaccctgaat
     2941 caaaaaagct ctatgaagaa gtgcagggtt ttaaggtcac tagatagtca tctttgctct
     3001 attattgaaa gactatagtt ttcggtgagg tatttatgtg cgactcttgc aacatctttc
     3061 tttgtcactt tcttaaccct gtcaaggtat ctgtctaaga gggctgcgtc ttttatcagc
     3121 tcccagaagc caagggaatc agcaagctct tttggatcgt cattttgcat gatatgctgg
     3181 ccgataagcg aattttttgc ttcatttaac tccttgtcgc ttagaccctt tagcacatca
     3241 aactgagtcc tgataagctc tgtaatcttt tttagattct ctttatcagt gttgaagtaa
     3301 acggcaaaga ccccatagtc aacaagggga tcatgatgaa cgcctacttc gtatgcgagg
     3361 ccatgcttgt ttcttatctc atcgaataat ctacctgact ggcccctgcc aagtatagag
     3421 cggatgacat ccaagacata ggagtcttca tcttttcttg gcgctgtctt gtagcccagc
     3481 acataataag aactcagaac ttttcttttt tcttttatga tattcttttt ttctgacgct
     3541 atactgatgg tttttggaat aatctggttt atgtttgctt ttttcgagaa tatgcttctt
     3601 ataaggtttt tatcgtttgg aatgttgccg actatggaga gaactgtgtt tgagggctga
     3661 taaaatttga tgaagaaatt ttgtaagtcg gtcctggtta tcttctttac tgaacccacc
     3721 aagccataag tcggaagacc tgcaggataa tctccaaaaa gcgcttttga aaaaagaatc
     3781 cattggtgaa acctggggtc atccttatga aggtttactt ccttaaggat aactttcctt
     3841 tccttttcaa gtttttcctt atcaaagaca gagttttgca gcatatctgc catgatatca
     3901 agggcaagat tgtaatgctc ttttggtacc ctgataaaga agcatgtcct ttcgctggat
     3961 gtataggcat taagatctcc accgatgctt tctatctcgt ttgagatagc ccttgcatcc
     4021 ggccttctct ttgtgccttc aaagagcata tgctccatga agtgcgagat tccgttgttt
     4081 tttttggttt cgtagttaga gccgactttt actgtgcatt gcacggttac agtatctatg
     4141 ggtttcttct gacttataac agtaagacca ttatcaagta tgaatttttc catatcgcaa
     4201 tgtttatata agaatttata tatttttccg tgatggctag aatcaaggaa ttccaggaaa
     4261 taatgaggaa gaagaaggtc gatatattcc ttgctatgaa tactgacttt tcaagacttg
     4321 agtatgatat ggcttacttt actggatatg gcagcatcgg cgccctgata atccctcaac
     4381 agcaaaagcc ttttctgata gtgcccagga tggaaaaaga aagggctaaa cgaagcggtg
     4441 tcttagtcta ctctccgcca aaggggatga ggatatctgc ctatgcaaat gagatactta
     4501 agaagaaagg agtcagaaca aaaaaggcag gaataaataa ggaagagatg actattgtta
     4561 taagggattc atttaggaaa gcattgaagg caaggtttgt cgacttgaga aaagatatct
     4621 ataagttaag ggagcagaag actgctgatg agataaagac tataagggaa ggatgcaaga
     4681 tctctgactc catcctggag aaatgcttta gggagtttaa aaattttcag acagaggcgg
     4741 ctgtaaaggc ttttcttgag atagaggcta agaaaagggg gtgtgagata gcgtttccta
     4801 ctatagttgc gtctggccct aatgcaagga tggcacatta tgatatatgc gataaaaaat
     4861 tgatgaacgg tttttgcatc atcgactatg gcataaggta taagaactat tgcaccgata
     4921 cgacaaggac agtctatata ggagagcctt cgaaaaaaga aattgaatta tataatcttg
     4981 tattgagctg ccagaggaag gcaatagaga gctctaagat aggagcaaag tgctcaaaga
     5041 tatacgaggg agcggttgct gatcttggaa aatacggaaa gtattttaca catgggcttg
     5101 gccatggatt tgggataaag gtacatgaag cacctgacct cacaccgaaa tctaaatata
     5161 ccctcaaaga atatatgaca tttactattg aacctggggt ttatcttgat aatctaggca
     5221 taaggataga agatgatatt ttgataactg acagaactga gatattaaca cgggctaaaa
     5281 aggagctagt ggtgattaca agagggagaa catgaaaaga tataatctga aggaacttga
     5341 agcagcatcg ttgagataca aaagggcact gggaaatata aaagggaaaa cagcattcat
     5401 cgtgctggat ataaggcaga aagatgcatt ttactctctt gcaccgttat cgaaagctat
     5461 ggcagagtta ggatgcgatc tcaatgtaca agctatcaac aagaagtctg aaagcataaa
     5521 tgcacttgca gatgtgtgga agtgctataa ggatctttct ggcggcgtta agaatgccag
     5581 gacaaaagca cttaaggatt ttttatatgc cgctgataag aaatgcaaag gaaagttaaa
     5641 tgggttgttt agggagccag aagtaagact taacgctacc gcaggaggtt ttaacggcag
     5701 ctatttcctt aagtatgaga ctgattggat gaatgagtat cgcaaaaagg atctggtaca
     5761 gaccgcaaag gtcatatgca aacaggtcta taaccttaag aaaaatgaga agataagcat
     5821 cggatttgac atccttagga agaaagaaga ccttggactt cctatcgagg actatcttga
     5881 ttctttccag ataagctatg ccatgatgga agctgttaaa aaccaggtga ctggaatggg
     5941 agcttatacc tctcgcagat cacagatgga tgaacctgag aggatagcgg accttaagtc
     6001 cacgcttctt ggctgcgagc tctgcaagga tgtagatgaa gcaattttca aaaaatataa
     6061 ggtattatca aaggccttgc ggcttgatag gataaaggtc gtttcaagcg cctctttttc
     6121 catacatggg aaaggatacg ccggaaggca ttattttgga gaatatatag gatatccttc
     6181 gaagaacaaa aagacgagat ggcttagccc tgggcagatg atctataagc tggattacta
     6241 tcctcagact gcacttgacc caagggctcc tatgacaagg ataggattta ccgagacgct
     6301 gcctatagat atattcatca agacgagcaa tattgactgg cttgcgatga agagaagaga
     6361 tgacaagata atagatatca ccaataaatg tgaaaagata attgtcgagg gaaagaagat
     6421 aggcaaatac cagacaaaac ttgagatagg gctcgtcaac aaaaataaga gaagaaagtt
     6481 taggggctct gatgttgaga caaggaatct gataaacccc tggtattaca aagagacagg
     6541 gataaaagca gggactatgg caaacatccc tggtggagaa gcattcgtga cccctgaata
     6601 tgtaaaaggg cagtttatcg gcgatgtggt gataagcctt gaccaaagct atgtgctttc
     6661 tggaaaagag cctatggtcg taagctgttt tgggaattca tataaagcag tttccgggcc
     6721 tcagaagata ttagagaagt ttaaacagaa gaaagatgag gcttggaaga aacttaagat
     6781 gcaggagaaa aacaagtcac tacctaaaca ggtgctggaa gtcaacaaga gaaactttaa
     6841 caatatcggg gagtttgcta tcaatacaaa ccctcaggca gagctatgca actaccttat
     6901 cgtaaatgag aagattgcaa acatgataca tcttgcgctt ggctcggggt ttgaggctga
     6961 tagggcaact tactaccata cagacatcgt aatagattct cctagacaac agcttgatat
     7021 ctatggggtt gcaaaggatg gcaaaaagca ctggatacac aggaaaggaa agtttgttgt
     7081 ctaaccttag agaagtatac agggttaatc tcgctgtcaa gaagaacgag agagtgcttg
     7141 tcgtaataga cagaagaaag agaaaactag gcaggctttt tcttgcagct ggcaaggact
     7201 ttagcccttg cattgatcta atggagattc ctgtcggcaa ggtaaatgga gaagagccgc
     7261 cttctgctgt cgccaagcag atgcaaaagc atgatgtgct gatacttctt acaacaaaat
     7321 cgttgacgca tacaaatgct agaaaaaacg cctgcaggaa gggtgccagg gttgcaagcc
     7381 ttcctgatgc tactgaggat atgcttaaga gaacactcag cgttgactat aggcttatgc
     7441 acaaaaggat agctgagata agtgatgtcc ttgacaaagg aaagattgtc agaatagtga
     7501 ctcctgcagg gactgatctt ctattcagca ttaaaggacg cagagcgtat ggacgagaaa
     7561 gcgggatatt taccaggaaa ggaagatatg gaaacctgcc tgatggggaa tcgtttatag
     7621 cgcctgttga aggcagcgct tctggagttt tcatcataga tggttcaata ggcagcatgg
     7681 gaatggtcga tagacctgta aaggtgtttg tgcaaaaagg gtctgtcttg aagatcgctg
     7741 gggggaaggc tgcaaaggtg ttgatgaaac agctcaatga tgcaggcaac aaagctaaga
     7801 atatcgctga gtttggaata gggatgaatg acagtgcaaa gatcacaggc aacatgcttg
     7861 aggatgagaa agtgatagga acatgccata ttgcgcttgg aaacaactcg ggctttgggg
     7921 gcaaggttga tgtgccactg catatagatt gccttattaa gaaaccagta atttatattg
     7981 acgaaaagtt acttagaata ccctaaatta tttaaatatc atctctaata gtgcataata
     8041 tggcctttaa tgacaggtta agaatctcat gggcagagta taaaggaggc ttgaaagcct
     8101 atttggcaat aacagctctc ttcatcataa tccctgattt gctaagatat ctcgttgcaa
     8161 tacctcttgg acttgaatat tatgcttctg gagcagacat attcaagtta ggctcgacca
     8221 tctttgagcc aaagtttctt atacctaata tgactatgtt aatcttgaat tttgtctcta
     8281 gaacatttgc cgttgcctcg attatctatt tttgtgttta cagaaggaag agttttatag
     8341 aggcattata tggaggaagg agatacctct taaagtgcat atggctatct ctggttttgg
     8401 gcttctttgt gtctggattg ctttttttac tggtaatccc gggaataata tttagcatct
     8461 attggagctt cagctatttt gtacttattg gaacaaacaa aggcgtgatt ggatcattaa
     8521 aagaaagccg cgagatcata aagggaaact ggtggaaggt gtttggatat ttgatctgct
     8581 ttgccttatt tgtaagtatc cttgccctgc cttttaagat aatcggagat gtagttataa
     8641 aaacctttat cggcttgtct actcctatca caaaagatat tctgagcaag atttttgaat
     8701 ggatccctca attgataacc attccattaa tagtattatt ccttaaaaac ctgtttcttg
     8761 agttgagaag ggaaaaattg atgatttacc aatctattcc aaccaagaat tggcattgat
     8821 agtaaacaat aaaacaccat caaaccaaag gtttttaaat tctacataag tttcttcaag
     8881 tatgcctcca tcagacagca gcaggaatat cttagacagc gtgatatatc gatacaccac
     8941 taactcttca cagcttgagg aggataacaa gatactcaaa gagactatca caaaactcca
     9001 aaaagaagtc gagaagttca agcttcctcc acttatggtg tgcgaggtaa gggagctgga
     9061 agatgaaaca gcaataatct cgatcccaaa tggaaaccag tttagtgttt gcatatctga
     9121 tacatgcgat aagattaggc cgggggatat ggtgcttgct gagcagaaaa acctggtcat
     9181 aatcaaaaaa ataccgattg cccgtaggtt caatgtcgag aaattcgtaa tcgtcgaaaa
     9241 accgactgta agctggaagg agataggcgg gcttgacaag caaataaatg agataaagga
     9301 agttgttgag ctgccactaa aaaagccaga gctgttcaag aaggtaggga taaagcctcc
     9361 aaaggggatc ctgctttatg gtccgcctgg cactggaaag acacttcttg caaagggagt
     9421 tgctgctgca actgacagca cattcataga gattgtcgca tctgaacttg tccagaaatt
     9481 catcggcgaa ggtgcaaagc ttgtaaaaga gatatttgag atggcaaggc acaaagcacc
     9541 agccattgta tttatcgatg aacttgattc acttgccgca aaaagggtag agataggcac
     9601 atcaggtgag cgagaggtaa acaggacatt catgcaactc cttgctgagc ttgacggatt
     9661 tgacccgctg gataatgtta aggtgattgg agcaacaaac aggaaagaca tacttgaccc
     9721 tgcgataatc cgaccaggaa ggcttgacag gctgattaat gtagggattc ctgaaaagga
     9781 agcaaaactg cagatactaa agatacactc aaaaaatatg actcttgaaa aaggtataaa
     9841 tttcaatacg ctggtagatt ccatggaaga gttctccggc gcagagataa gggctgtctg
     9901 cactgaagcg ggatattttg ctatcaggaa taacaggaca agcattataa gagaggattt
     9961 ttccaaagcg attgagaagg tcaagaaaga tgagaacgct gaaggtgatg attacttaag
    10021 gatgtttggc tagggccttg agtatttttt ctttgttttc tagaagtaat ggatagataa
    10081 cttgtgtttc cctgataaaa tgtcctattt tgaccatacg ctgtatctca tcaatatcaa
    10141 tgaacctgcc actttctatc tcggtcttat ccaatatcgg ttcatggtcg atgatgaagt
    10201 aatagagcgc agcattttcc ttgtcttttt ccttgaatct gctcttgaag tttgcgaggt
    10261 agtggggctt tgaggtaatg cctgcttctt cttttgcctc tctgatcact gtttgttcat
    10321 aagagtcgcc ttttattaca tggccgccaa gaacaaggct aaatacacct ggctcaaatt
    10381 cttttttcaa agaacgccta ttcataaaaa gctggccttt tttattgaaa ataaagaata
    10441 tcccactttt gtggagaaaa cctttttcgt gggcttcttt tcttgttgca gtgcctatta
    10501 tgttgttgta gtcatcgact atatcaaaga gttcttgcat gaagggatgg attaatggag
    10561 gtataataac ttattgcagt tatttattaa atcagccaaa gatctgattt ccaagttatt
    10621 atgcactttg aatatattgc aagggtttaa ataaggagca aaaagttaag actagcatgg
    10681 ctgaatatat cgagttgaat gcgttcataa agatgaaagg ctttgctgca actggaggcc
    10741 aggctaaggt tttgataaga tctggtgctg tcaaagtcaa tgggcaggtc gatacaagga
    10801 acaagaaaaa attgacctcc accccaaccc tgaagggtgg ggattccgtc gaattattgg
    10861 catgatgagt ctcttgcttc tctaga
//
LOCUS       DASRUK010000134         7733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_445518, whole genome shotgun sequence.
ACCESSION   DASRUK010000134 DASRUK010000000
VERSION     DASRUK010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 7733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7733
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445518"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..618
                     /locus_tag="VFF28_06005"
     CDS             160..618
                     /locus_tag="VFF28_06005"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZX45208.1"
                     /translation="MEGKMNKEIDETDKKIINILLYDADATNREIALKCSIALGTVNN
                     RLKRLKKLGILLKKTVQVDYEKLGYLIEVLIDIKIKKGGFYDLAKKLSADPNVFMVID
                     TTGDYDAEIIARFHTTRQLDTFVKKLQHEQAVEDTRTRLILNIYREKEIR"
     gene            complement(615..1193)
                     /locus_tag="VFF28_06010"
     CDS             complement(615..1193)
                     /locus_tag="VFF28_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HZX45209.1"
                     /translation="MRPMVKGTQTEKNLLKAFAGESQARNRYTFFSSIAKKEGYEQIA
                     AIFQETADNEKEHAEIFFKHLVGGALEINASFPAGMIGKTKDNLLAAANGEKEEWSKL
                     YPSFEKVAGEEGFPAVAASFKEIAEVEEHHEQRYRQLLTNIEQNKVFSKGSQVKWKCR
                     NCGYVHEGKDAPLVCPACKHPQAYYELLADNF"
     gene            1300..1548
                     /locus_tag="VFF28_06015"
     CDS             1300..1548
                     /locus_tag="VFF28_06015"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZX45210.1"
                     /translation="MDRLTLFKALSDKTRLSIVSLLLDGEKCVCEIFPHVKRTQSTVS
                     TQLGKLEKWGILKSRRQGKWMFYSIKDKRIYSIFKAVR"
     gene            1545..2594
                     /gene="arsB"
                     /locus_tag="VFF28_06020"
     CDS             1545..2594
                     /gene="arsB"
                     /locus_tag="VFF28_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HZX45211.1"
                     /translation="MMEERKLDFFSKYLSIWVAICIILGTAIGYFFPAVSGILGGVEV
                     ANVSIPVAIVLLLMMYPIMLKISFKEIAKVKNNHKPLWLTLTINWAIKPFTMAFIAWL
                     FMRFIFAGFIPANIQTEYIAGMILLGLAPCTAMVLVWTYLAGSNINYALIQVAINDLV
                     ILVLFAPLGKLLLGVTTDFPVPFGTIFFSVLFYVAIPLAFAGLTRFCLIRKRGEQWFN
                     ERFIKSIEWITPAGLLVTLILLFIFQGDKIIHYPLHILLIAIPLVLQTYIIFYIGYFG
                     AKKLKIPYSEAAPSSFIGASNFFELSVAVALILFGMNSGATLATVVGVLVEVPVMLHL
                     VKIMKNNKQKFGMIS"
     gene            2591..2818
                     /locus_tag="VFF28_06025"
     CDS             2591..2818
                     /locus_tag="VFF28_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZX45212.1"
                     /translation="MKIEILGSGCPNCKRLEQNTRTAVEKAKVKADIIKVTDIGKIIE
                     YGVMSTPALIIDGKLKCYGRIADVQEIKKWL"
     gene            2819..3802
                     /locus_tag="VFF28_06030"
     CDS             2819..3802
                     /locus_tag="VFF28_06030"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HZX45213.1"
                     /translation="MDIFYPVQWLADKASYQWLSLGKESYLGSAANFFIYDTIKIGIL
                     LILINYLMAVIRYFFPTNKIRDILAKRRWFGLDYLFAALLGMITPFCSCSSIPLFIGF
                     VGAGIPLGVTFAFLISSPLINEASLYLFPAMFGIKATLIYNILGITVAIAGGMLIQRL
                     KVDKYVRQELLKIKTRQQTENGGSRPTVKELASYFWKDGWHLTKRIFPYVVLGVGVGA
                     LIHGFVPVSFVERYLSSKSWWTVPFAALLGAPLYANSVGVIPIMEALVGKGVPIGTAL
                     AFMTSIVTISLPELLILRKVMKWQLLALFMAITLVGIVIMGYVLNALLVIG"
     gene            3799..4257
                     /locus_tag="VFF28_06035"
     CDS             3799..4257
                     /locus_tag="VFF28_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HZX45214.1"
                     /translation="MKAIIIILLCVVLSACATVKEDSHISVTTSNVAAASPVEKVEVY
                     HFHGTNQCSSCIAVGKLAEETVNAYFADELKTGKVAFAHINYDLLENKELASKYGVTG
                     SSLWIGVYDKQGFHAEQNTNVWYKINNKKDYMDYLKETIEQKLSRGFAIG"
     gene            4254..4961
                     /locus_tag="VFF28_06040"
     CDS             4254..4961
                     /locus_tag="VFF28_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HZX45215.1"
                     /translation="MSLLSFMETMGASSIPIVAAFFIGLMTAISPCPLASNITAIAYA
                     SKRINNSRHTLLVGFLYTFGRMITYVLIASLIVWFGLSTQAIALFLQRYGEKLLAPLL
                     IIVGILMLDVIPLRFGGGQKLERIKEKIAGKGLFGSFVLGVVFALAFCPFSAVLFFGM
                     LIPLALRAGDGIIIPSVFALATGLPVIIFSFVLVHSVSKLGKVLNKLQSFEKWTRRVV
                     AILIILAGIYFLIRGFA"
     gene            4958..5707
                     /gene="arsM"
                     /locus_tag="VFF28_06045"
     CDS             4958..5707
                     /gene="arsM"
                     /locus_tag="VFF28_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HZX45216.1"
                     /translation="MKNSRQTDVKKIVKQRYGKIADTGCGCSCGCNSMDNEQIAKSIG
                     YSDEDIKAVPSANMGLGCGNPTALGEIKEGMTVLDLGSGAGFDCFLAAKKVGKTGKVI
                     GVDMTEKMLAKARENARKYGYANVEFRHADIEELPVEDNSIDVIISNCVINLAPDKSR
                     VFKEAYRVLKKGGKMLVSDIVLLKELSKKDREDEGLVAGCVGGAMLRDEYLKIIKDAG
                     FSIDIISEDKEISKRQYQGIALESLKIEIKK"
     gene            complement(5852..6004)
                     /locus_tag="VFF28_06050"
     CDS             complement(5852..6004)
                     /locus_tag="VFF28_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45217.1"
                     /translation="MESIARIIVGEAALCAASVTDAGLKQVAKEHLNEAKQWADYLAQ
                     QKQIYQ"
     gene            complement(6341..6538)
                     /locus_tag="VFF28_06055"
     CDS             complement(6341..6538)
                     /locus_tag="VFF28_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45218.1"
                     /translation="MPADGFFFSYRGKDKSNPLVNSDKLDEAERFRKALSQIKYGLER
                     EERIEDFEDREEDLSYASSEA"
     gene            complement(6599..7519)
                     /locus_tag="VFF28_06060"
     CDS             complement(6599..7519)
                     /locus_tag="VFF28_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45219.1"
                     /translation="MTDVEQEWTRFMDSLPNFKQRILKCAELLPNAEMGQPYVRKMID
                     SVMPDIHQGFSSEETLLVSSILMYSGNDSFVSLADLLIQFETLPQLPTRTNEPVKDIE
                     SLLSYCESLGIEEALRKAQHLYDKAQNAYGSSLGILEKVSICAERNSVEKKVCQKSSE
                     MLISYLQFHREIILLMNSAAETAIGYANSTDQSIPADQYQEIRGSILALLQSAINSEL
                     IIRSSSGLNPRLYDAVTRSPLYKGLVDQVTQMHKKTVDLQKTLDKSLPPNTEAGETAN
                     FARTYGDPLQMKPAGNCLDLIQRIIDHLNQ"
BASE COUNT         2277 a         1474 c         1705 g         2277 t
ORIGIN      
        1 agaatatcta gagaagcaag agactcatca tgccaataat tcgacggaat ccccaccctt
       61 cagggttggg gtggaggtca aatacctttt ccatctctac ctgaacaaat caccaaaaaa
      121 tccataaaat ttattaatta gttcagcgcc caataaggaa tggaaggaaa aatgaacaaa
      181 gaaatcgacg aaactgacaa gaagataata aacatcctcc tctatgacgc agatgcgaca
      241 aacagggaga tagcacttaa atgcagcatc gcccttggca cagtaaacaa ccgcctcaag
      301 aggctaaaga aactaggtat cctgctaaaa aagacagtac aggttgacta tgaaaaatta
      361 ggttatctaa tagaagttct gatagacata aagataaaaa aaggtggatt ctatgacctt
      421 gcaaaaaagc tgtcagcaga ccctaatgtg tttatggtaa tagatactac tggtgactat
      481 gacgccgaga taatcgcaag gtttcacacc acaaggcagc ttgacacttt cgttaagaaa
      541 ctacagcacg aacaagcagt ggaagacaca aggacaaggt tgatactaaa tatttataga
      601 gaaaaagaga tacgttaaaa attatcagcc aagagctcat aataagcctg aggatgcttg
      661 caggcagggc aaaccaatgg tgcatctttt ccttcgtgca catatccgca gttcctgcat
      721 ttccatttta cttgggaacc cttggaaaag accttgttct gttcgatatt agtaagcaac
      781 tgcctatacc tttgctcatg atgctcttcg acttcagcta tctccttaaa agacgcagca
      841 acagcaggaa atccttcctc ccccgcaacc ttctcaaaac ttggatagag ctttgaccat
      901 tcctccttct cgccgttggc agcagcaaga aggttgtctt tcgtctttcc gatcatcccg
      961 gcaggaaacg aagcgtttat ctcaagggct cctcccacaa gatgcttgaa aaatatctca
     1021 gcatgctctt tctcgttgtc agctgtctcc tgaaatatcg ccgctatctg ttcataccct
     1081 tctttttttg caatggaaga aaaaaatgtg tatctgttcc ttgcctggct ctctcctgcg
     1141 aaagctttaa gcaggttttt ttcggtttgt gttcccttta ccatggggcg tattaattat
     1201 tcctttctta ttaatctttc gattggaggc tacaatgtct gcaggccaac ctaatctaga
     1261 tggcaaaatt tatatattga tggctatcaa tatgttttta tggatagatt aacactattc
     1321 aaggcattgt cggacaagac cagactttct atagtctccc tcctccttga cggagaaaaa
     1381 tgcgtgtgtg aaatattccc tcatgtcaaa aggacccaat cgactgtttc cacacaattg
     1441 ggcaagctgg agaaatgggg catccttaaa tctagaagac aaggcaagtg gatgttttac
     1501 agcatcaagg ataaacggat ctactcgatc tttaaggcgg tgagatgatg gaggagagaa
     1561 aactggattt tttcagcaaa tatctgtcta tatgggtagc aatctgcata atacttggaa
     1621 cagccatcgg atactttttt ccagccgtat ccgggatttt gggcggtgtg gaagtggcaa
     1681 atgtctcaat tccggtggct atagtcctat tgctgatgat gtatccgata atgctaaaaa
     1741 taagctttaa agagatagca aaagtaaaaa ataatcacaa gcctctgtgg ttgaccttaa
     1801 ccataaactg ggcgatcaaa cctttcacta tggctttcat cgcatggctt tttatgagat
     1861 ttatatttgc gggttttata cctgctaaca tccaaacaga gtatattgca ggaatgatat
     1921 tactagggct tgctccctgt acagcgatgg tacttgtctg gacatatctt gcagggtcaa
     1981 acatcaatta tgctttgata caggttgcaa tcaatgactt agtaatactt gtgttgtttg
     2041 ctcctttggg taaactcctt cttggagtta cgaccgattt tccagtccct tttggcacaa
     2101 tttttttctc ggtcctgttc tacgtcgcca taccgttggc atttgccggg cttacacgat
     2161 tttgccttat cagaaagaga ggggagcaat ggtttaatga aaggttcatc aaaagcatcg
     2221 aatggataac acctgctggc ttacttgtaa cattgatact gctttttatc tttcagggtg
     2281 ataagataat ccattatcct ttgcatatct tattgatagc tattccacta gtattgcaga
     2341 cttatattat attctatatc ggatattttg gtgcaaaaaa gcttaagata ccctatagcg
     2401 aagctgcgcc ttcatccttt atcggagcaa gcaacttctt cgagctgtct gtcgctgttg
     2461 cattgatact ttttgggatg aattcgggag ctactttggc tacagtcgtt ggggttttgg
     2521 tggaagtgcc ggttatgctt catctggtga agataatgaa aaataataaa cagaaatttg
     2581 gaatgatatc atgaaaattg agatattagg ctcaggatgc cctaactgta aaagacttga
     2641 gcaaaataca cgtacagcag tagagaaagc taaagttaaa gcagatatca tcaaagttac
     2701 agatatcggc aagataatag agtatggagt gatgtcaaca cctgctctca tcatcgacgg
     2761 caaactgaaa tgctacggca ggattgcgga tgtccaagaa ataaagaagt ggctctaaat
     2821 ggatatattc taccctgtac aatggcttgc agataaggcg agctatcaat ggctaagcct
     2881 gggaaaagaa tcctatttag ggagtgctgc caactttttt atctatgaca caataaagat
     2941 aggcatcctt cttatactca tcaattatct tatggctgta atcaggtatt ttttccctac
     3001 aaacaaaatc agggacatac ttgcaaaacg caggtggttt ggtctagatt atctttttgc
     3061 agcgctcctt ggaatgatca ctcctttttg ctcatgctct tcgataccgt tatttatagg
     3121 ctttgtcgga gcaggaatac ctttaggtgt aacctttgca ttcctaataa gctctcctct
     3181 tatcaatgag gcatcccttt atctttttcc agcgatgttt gggataaagg cgacgcttat
     3241 ctataatatc cttggaatta cagtagctat agcagggggt atgcttatac aacggcttaa
     3301 ggttgacaaa tatgtcaggc aggaattatt gaaaatcaag accagacagc agacggaaaa
     3361 tggcggaagc agaccaactg taaaggagct tgcgtcttat ttctggaaag atggatggca
     3421 cctaactaag cggatcttcc cctatgtagt cttaggggta ggtgtcggtg cattgatcca
     3481 tggttttgtt cctgtaagct ttgttgagcg atatctatca tcgaaatcat ggtggactgt
     3541 cccttttgca gcactacttg gagcaccgct ttatgcaaat tcagtcggag tgatacctat
     3601 catggaagcc cttgtcggta aaggggtgcc tataggtaca gcgcttgctt ttatgacgtc
     3661 gatcgtcaca atatcacttc ctgaactgtt gatactaaga aaggtgatga agtggcagct
     3721 tcttgcccta ttcatggcaa ttaccttggt cgggatagta atcatggggt atgtgttgaa
     3781 tgcccttttg gtgataggat gaaagcgata attattattt tgctctgtgt tgttttgagc
     3841 gcatgtgcaa cggtgaaaga agacagtcat atcagtgtta ctacttccaa tgtagctgcg
     3901 gcaagtcctg tggagaaggt agaagtctat cattttcatg gaaccaatca atgcagctcc
     3961 tgcattgcgg tcggcaaatt ggcagaagag actgtcaacg cctattttgc tgacgagtta
     4021 aagactggaa aggttgcctt tgcacatatt aattatgatc tcctggagaa taaggaactg
     4081 gcatccaaat atggcgttac aggctcttca ctttggatag gggtatatga taaacaggga
     4141 tttcatgctg agcagaatac caacgtatgg tataagataa ataataaaaa ggactatatg
     4201 gactatctga aggaaacaat cgagcaaaaa ttaagcagag gttttgcaat aggatgagct
     4261 tactctcttt tatggagacg atgggggcaa gcagtatccc tatcgtggct gcatttttta
     4321 ttgggcttat gactgcgatt agcccttgcc ctttggcatc aaacatcact gctatcgcat
     4381 atgcttctaa gaggataaat aacagcaggc atacgttgct ggtaggattc ttgtatacct
     4441 ttggaaggat gatcacttat gtcttgattg cttctcttat agtctggttt gggttgagca
     4501 cacaagcgat cgcattattc ctgcaaagat atggggagaa actgcttgca ccattgctta
     4561 tcattgtggg catacttatg ctggatgtta tccccctgag gtttggcggt gggcaaaaac
     4621 tggaaagaat taaggagaag atagccggaa aggggttgtt tggcagcttt gtcctggggg
     4681 ttgtctttgc tcttgcattc tgcccgttta gcgcagtgct gttctttggg atgcttatcc
     4741 ctcttgctct tagggcaggc gatgggataa taataccttc tgtatttgca cttgcgactg
     4801 gtcttccagt cataattttt tcatttgtat tggtgcatag cgtttcaaag ctgggcaagg
     4861 tattaaataa attgcagtct tttgagaaat ggacaagaag ggtagttgca atattgataa
     4921 tcttggcagg aatatatttt cttatcaggg ggtttgcatg aaaaacagta gacaaacgga
     4981 tgtaaagaaa atcgtcaaac aaagatatgg taagattgca gacacaggct gcggctgctc
     5041 ttgcggatgc aattcgatgg acaacgaaca gatagcaaaa agcataggat attctgatga
     5101 ggatattaag gcagtgcctt cagctaatat ggggcttggc tgcgggaatc caactgcttt
     5161 aggtgagata aaagaaggga tgaccgttct tgaccttgga agtggtgcag gatttgactg
     5221 ctttcttgct gcaaagaagg tcggcaagac aggaaaagtt atcggtgtag atatgacaga
     5281 gaagatgctt gcaaaagcaa gggagaatgc caggaagtat ggctatgcaa atgttgagtt
     5341 caggcacgca gacatagaag agcttccggt tgaagataat tccatagatg tcatcataag
     5401 caactgcgtg ataaaccttg ctccggataa gtcaagggtg ttcaaggaag cttacagggt
     5461 tctaaagaaa ggaggaaaga tgcttgtttc cgatattgtg ctgctaaaag agctgtctaa
     5521 gaaagacagg gaagatgagg gattggtagc tggctgtgtc gggggggcga tgcttaggga
     5581 tgaatatcta aagataataa aagatgcagg atttagcatt gacattattt ctgaagacaa
     5641 ggagataagc aaaaggcagt atcaaggcat cgctttggaa agccttaaga tagagataaa
     5701 gaagtgattt ttgatgcctc tgacggctcg ttaaactcac taaacgtgcg gatttcgaca
     5761 gaaaagtttt cgcaatcgcc atttagtctc agagcgaagt gatatatcac gttaaaggat
     5821 taaggggtaa aagtaaaacg tctagaatat tttattgata tatctgtttt tgttgtgcta
     5881 aataatcagc ccattgtttc gcttcattca ggtgttcttt agctacttgt ttcaaaccag
     5941 cgtctgttac acttgcagca cacaaagctg cttctccaac aatgattcga gcaatagatt
     6001 ccattgtatc aacagtttaa atgcattatt aatcttagaa taagtgatta ttatgtttgc
     6061 ggagtggggg ttgaccaagc aacaaagttg cgaagagtta tgacggaaga aaaattttcc
     6121 cacttaaagc tcgaagagca attaagtaaa agaagggaag ggcaaaaaga attatcttcg
     6181 tgcatcaaca gcaacagcaa aaaaagcctt gaatttagcg aagctaaatt ccaccttttc
     6241 cccaggcttt ttgctgaagg gcaaaaaggc tggggggcaa tcaacgtggc cactttttta
     6301 ctctttaaga tgataagaaa tagcacagca agcttgtgct ttaagcttcg ctgcttgcat
     6361 aactaaggtc ttcttccctg tcttcaaagt cttcaatcct ttcctctctc tcaaggccat
     6421 attttatctg gctcaaggct ttcctaaagc gctctgcctc atcaagtttg tctgagttca
     6481 caagcggatt actcttgtcc tttcccctat acgaaaagaa aaaaccatct gccggcatag
     6541 caatcacccc cctttaattt gcaagatagc gtaaggtttt tgggtattta aaattatctt
     6601 attgatttag atgatcgata attcgttgga ttaggtcaag acaattccct gctggcttca
     6661 tttggagagg gtcaccataa gttctggcaa agttagccgt ttctcctgct tcagtattag
     6721 gaggcaggct tttatccagg gtcttttgca ggtccactgt tttcttgtgc atttgggtca
     6781 cttggtcgac caacccctta tagaggggtg agcgagttac agcatcataa agacgaggat
     6841 tcagtccgct gctggatcgg ataatcagtt cagagttaat agctgattgt agcaaggcca
     6901 gtatacttcc cctaatttct tgatattggt cggcaggaat gctttgatct gtgctatttg
     6961 catatcctat cgctgtctct gccgctgagt tcataagaag tataatttct cgatggaatt
     7021 gaaggtaact gatcaacatt tcagacgatt tttgacatac ttttttctca acagagtttc
     7081 tttcagcgca tatgcttacc ttttccaaaa tacccagaga tgacccatat gcattttgtg
     7141 ccttatcata aagatgctga gccttcctta aagcttcttc aatgccaagt gactcgcaat
     7201 atgaaagaag gctttcaata tccttgacag gctcatttgt acgagtgggc agctgaggta
     7261 aagtctcaaa ttgtatcaac aagtccgcca aagagacaaa cgaatcattt cctgaataca
     7321 ttaatatcga ggataccaat aaggtctctt cagacgaaaa accttgatgt atgtcaggca
     7381 tgacactgtc gatcattttc ctgacatagg gttgtcccat ctcagcatta ggcagtagct
     7441 cggcacattt taatatcctc tgcttaaaat taggaaggct gtccataaac cttgtccact
     7501 cttgctcaac atctgtcatg atagatagga aattgaatct ttgtttataa attttgtcat
     7561 taaatagtgg taataaacaa aaattcaatt taagagtatg actactgaaa cgaaagatat
     7621 aaatataagc taattctaaa aatatatatt attgttcgtc aataataaag gtgatatcat
     7681 gccaaagaaa agaaggaaat tagatgtctt ctgccaaaat cagaaatgcg agc
//
LOCUS       DASRUK010000135         2715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_277708, whole genome shotgun sequence.
ACCESSION   DASRUK010000135 DASRUK010000000
VERSION     DASRUK010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277708"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1676)
                     /locus_tag="VFF28_06065"
     CDS             complement(<1..1676)
                     /locus_tag="VFF28_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HZX45220.1"
                     /translation="MCGIVGIFNRKDAGKLVVDALEVIKYRGKDGFGACTDNECNYSK
                     TLTGLKVKASASAIGHALHSVVSLAKQPFVGKGKFIVNCEIYNWKELNEKYRFNASND
                     AEALFKLIERNGVENAIKELDGVYAFAYWTGNELCLARDILGEKPVWYSHKDGFAFAS
                     EKKALAKLGYMDAVELNPRQILKYDISGDKVSFVQREFFSITPEDKRSKERIKVDLKE
                     KIIRAISKRIPDKKFGLLLSGGVDSALIAYLLKQMGHKFTCYVAVLDEPGMSEPEDLY
                     YAQKIAKELRLKLKVIKIKLKDAQKYLKQIVPLIEDSNVVKAGVGLTFFAACKQAQKD
                     RIKIIFSGLGSEEIFGGYQRHKNSLDINKECLSGLLKIYERDLYRDDVVTMYHNIELR
                     LPFLDTSLVDFALKIPAKHKIVGEHGKYILRELASDMGLSSEFAWRKKRAAQYGSKFD
                     RAIEKLAHINGFKYKSEYLRQFYPGHNLRLGALISSGKDSLYATYVMLRQNYSADCFI
                     TLVSKNPDSFMFHTPNIHMVKLQSECAGIPLIEQETLGEKEKELRDLETA"
     gene            1729..1926
                     /locus_tag="VFF28_06070"
     CDS             1729..1926
                     /locus_tag="VFF28_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45221.1"
                     /translation="MITPQAKNYKPGKLTREMARQPHQKRVDISSLFQVRPADALETL
                     VYTAYCSKPQKPNPTFTPGQK"
     gene            complement(1994..2464)
                     /locus_tag="VFF28_06075"
     CDS             complement(1994..2464)
                     /locus_tag="VFF28_06075"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZX45222.1"
                     /translation="MKTNPVRYSSQVGEVWAHFMFKLKYCHNIFDNFGYRNACHGLFI
                     QALRKYEIRCKDNEIGFDSNHVHSMLDLGIKSKPEIAKKIKGYIARKFFRLFPELKLP
                     REQGGKFWDSGLWSPASYGATPTSLAFTVNYIKTQKYGSARDHAKQYRLTLFCD"
BASE COUNT          753 a          638 c          495 g          829 t
ORIGIN      
        1 atagctgtct ccaggtccct gagctctttt tctttttcac ccagagtctc ttgctctatt
       61 aatggaatcc ccgcacactc agattgcagc ttgaccatat ggatattagg cgtatggaac
      121 atgaaagagt ctggattctt gctgaccaag gttatgaagc aatcggcaga ataattctgt
      181 ctaagcatga cataggttgc ataaaggctg tctttgcctg aggatatcaa agctccaagc
      241 cttaaattat gccctggata gaactgccgc aaatattccg acttatactt aaatccattg
      301 atatgtgcaa gcttctctat cgctctgtca aacttgctgc catactgtgc tgctcttttc
      361 ttgcgccaag caaattcaga agaaagcccc atatcagaag caagctctct caggatatac
      421 tttccatgtt cgccgacgat cttatgtttt gcaggtatct taagcgcaaa atctacaagc
      481 gaggtatcca ggaatggaag tctcagctct atattatgat acatcgtaac tacatcatct
      541 ctataaagat ccctttcata tatcttcaac aatccagaca ggcactcttt attgatatca
      601 agcgagtttt tatgtctctg atatccgcca aatatctcct cagagccaag ccctgaaaag
      661 ataatcttga tcctatcctt ctgcgcttgc ttgcaagctg caaaaaaagt caggccaacc
      721 cctgctttga ccacattgga atcctcgatt aagggaacga tctgtttcag atatttttgg
      781 gcgtccttga gcttgatctt gatgaccttc aatttgagcc taagctcttt ggcaatcttc
      841 tgggcataat acagatcctc gggttcactc atccccggct catctaaaac agcaacatag
      901 caagtgaact tatgccccat ctgctttaac aagtatgcga tcagtgcaga atcaaccccg
      961 cctgacagca aaagcccaaa cttcttgtca ggaatccttt tcgagattgc tctgattatt
     1021 ttttctttca ggtcaacctt tattctctcc ttagatcgtt tatcttcagg ggtaatcgaa
     1081 aagaattctc tctgaacaaa tgaaacctta tcgccggata tatcatattt caatatctgc
     1141 ctaggattta gctcgactgc atccatatat cctaacttag ccaatgcctt cttctcagag
     1201 gcaaacgcaa atccatcttt atggctgtac catactggct tttctccaag tatatctctt
     1261 gctaaacata gttcattgcc tgtccaataa gcaaatgcat agactccatc tagctccttg
     1321 atggcattct ccacaccatt tctctcgatt agcttgaaca aggcctcagc atcgttacta
     1381 gcgttaaacc gatacttttc attaagttct ttccaattat atatttcgca attaacgata
     1441 aacttgcctt ttccaacaaa tggttgtttg gccaaactta caacagaatg aagtgcatgc
     1501 cctattgcac tggcagaagc cttgactttt aaaccagtta gtgttttcga ataattgcat
     1561 tcattatccg tgcaggctcc aaatccgtct ttgcctctgt atttgataac ctctagagca
     1621 tcaactacta gcttgccagc gtctttacga ttgaaaattc caacaatacc gcacatagcc
     1681 ttcaaggatt atgaagaata ttaaaaggta tctgataatg ataagaaggt gataactcct
     1741 caagcaaaga actataagcc tggaaaactc acaagagaga tggcccgcca accgcatcaa
     1801 aagcgagtag atatatcttc cttattccag gtaaggccag ctgatgcttt ggagactctg
     1861 gtatacactg cttactgctc aaagccgcaa aaacccaatc ccacgtttac gcccggacaa
     1921 aaatagaatc aagaaccacc agtcatcgct ctttgaccgc tatagactgg tggtatggca
     1981 agtgctcatt ttttcagtcg cagaataaag ttaatcgata ttgtttagca tgatctcttg
     2041 cagagccata tttttgtgtc ttgatataat tcacggtgaa agctaaactt gttggtgttg
     2101 caccgtagga agcagggctc caaaggccgc tatcccagaa tttgccgcct tgttctcttg
     2161 gaagcttcaa ctcaggaaaa agcctgaaga attttcttgc gatgtaaccc ttgattttct
     2221 ttgcgatttc tggcttgctt tttattccta gatcgagcat actatggaca tgattgctgt
     2281 caaagcctat ctcgttatct ttgcatctta tctcatactt cctcagcgcc tgaatgaata
     2341 agccgtggca ggcattccgg tagccgaagt tatcgaatat attgtggcaa tacttgagtt
     2401 taaacataaa gtgagcccat acctctccaa cttgagagct gtacctgact ggatttgttt
     2461 tcataagaac cacctcaaaa gttttttata gctaaagcta gtttagaatt aattgccaga
     2521 agctagatcg tacctattcg ccttggtaag cttatgacct ctagcgcttt acgaaaagtg
     2581 cccaagaggt atttcagtgg tttaggtctg cttcctggtt tttcttaagc ttttgataga
     2641 aatatgagtg ataagcataa aaagttcatg gttgcgcccc accagtcacc gctctttgac
     2701 cgctatagac tggtg
//
LOCUS       DASRUK010000136         6165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_946194, whole genome shotgun sequence.
ACCESSION   DASRUK010000136 DASRUK010000000
VERSION     DASRUK010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 6165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6165
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946194"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..1961
                     /gene="dnaK"
                     /locus_tag="VFF28_06080"
     CDS             30..1961
                     /gene="dnaK"
                     /locus_tag="VFF28_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HZX45223.1"
                     /translation="MVKKEKIIGIDLGTSNSAAAVLQAGKPTIIPSAEGTTQYGKAFP
                     SVVAFTKDGQVLVGEPARRQAVSNPEGTITAAKRKMGSNFKYNINSKEYTPQQISAFI
                     LQKIKKDAEEFVGEPIKKAVITVPAYFDDDQRQATKDAGEIAGLEVVRLVNEPTAASM
                     AYGLDKTDKDHKILVFDLGGGTLDVTIMEFGEGVFEVKSTSGDTQLGGTDMDVSIMNY
                     IAEDFKIKNDVDLSDNDTAMQRLRESAEKAKIELSTVTETDINLPFLTEKDGTPIHLQ
                     MRINRAKLEELVSPIIRRCKHPLEQAIRDAKLSLSDIDRVILVGGPTRMPIVRKFVED
                     YAGKKVERGVDPMECVAQGAAIQAGILAGEVKDILLLDVTPLTLGIETLGGVRTELIP
                     RNTTVPTKKSQIFSTASDNQPAVTINVLQGERPMAQDNKSLGRFDLVGIPPAPRGIPQ
                     VEVTFDIDANGILHVSAKDLGTGKEQKIQITASQKLNSEEIEKMKKDAEAHSEEDKKR
                     QQEIEAINQADSLVYTTKKLFKDFEGKVDSKELETVKGKILELEELLKPEKKDSAAIK
                     KKMDEVNELVQKMSTEMYQKAAAEAAKKGKGQAGPAPGDEEGGEDPSKKPKDDNVVDA
                     DYEVEGDKKEEGKKKGKSK"
     gene            2281..2796
                     /locus_tag="VFF28_06085"
     CDS             2281..2796
                     /locus_tag="VFF28_06085"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HZX45224.1"
                     /translation="MKILAINGSHRKGNTDFLLSNLLEDTDIIRLREKKITFCGGGDD
                     CCPKTGTCYIKDDMPEIEKKLVEADLVILASPCYFFNVTAMMKNFMDRCNPFFFNLKL
                     KGKKFFLIPLGGHPNSLPDALVCMKNFVKGIHGEAVGEYSAVANRRGDMSKNEKVIKE
                     LEDLKSRIKNL"
     gene            2798..3910
                     /gene="dnaJ"
                     /locus_tag="VFF28_06090"
     CDS             2798..3910
                     /gene="dnaJ"
                     /locus_tag="VFF28_06090"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HZX45225.1"
                     /translation="MAKDYYKTLGVGKNATKEDVKKAYKKLARQFHPDINKQADASDK
                     FKEINEAAQVLGDDEKRRQYDQFGDADSYKQASGFRGFDSSDFGFGDSDFASFDFGDI
                     FDKFFGGGGSFGGRRGRRAQRGSDLRYDMEITLEEAAFGVEKEIIIPKNETCPTCGGT
                     GAKTSDDIIDCPECGGQGVVRRTQRTPFGLFSTTTTCPKCHGTGKFIKIECPTCDGTG
                     IVHERKKIRLKIPAGSEEGTNLRVGGEGEPGARGAPSGDLYIVIHVKEHDTFERRGDD
                     IYLRINIPFTLAALGGELEVPTLEGKAKLKVPSGTQSGTVFKMRDLGIPFLHGHGKGD
                     ELVEVGIEVPKKLSSKQKKILEEFQKESPKKGFFDL"
     gene            complement(3922..4824)
                     /locus_tag="VFF28_06095"
     CDS             complement(3922..4824)
                     /locus_tag="VFF28_06095"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF002243.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide adenylyltransferase"
                     /protein_id="HZX45226.1"
                     /translation="MNIDECIARGFLQRIEPDARIEEKELRESDYDFGKAEKAFEDED
                     YKWSIIKSYYSMLHAARAVLFNLGFREKRHFAVSAVLEDLNKKGVIESRAVNDFSAAV
                     SSREDADYHYIHTRDGAKTSLLIADEFIERMKSILKQDKIALFIGRFQPFHKAHLSDV
                     KLALKECSKVIIAIGSSQESGTKENPFTYEERREMIKDTLEAHNVSSYDIVPVPDIFN
                     DEKWVEHVRKVVPEFDIVYTGNEFTAKLFLERKTEVRRIQLIPDINATEIRKRIICSK
                     DWEELVPKEVFDYIRKINGGRLDS"
     gene            complement(4821..5360)
                     /locus_tag="VFF28_06100"
     CDS             complement(4821..5360)
                     /locus_tag="VFF28_06100"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZX45227.1"
                     /translation="MVQLIRLIGNNKVLKIISFFFSNQTREFSQTELLAKIKIAKATM
                     IKCLKLMQEEKIISMKKIGVTNLYKLNNENTIVKHLKILFSLSELEPIKELSEKHNSK
                     IYLYGSAARGENIESSDIDILVIGAPDKSSLMSDIINISKKLDKEIRVQVSSNQEWSL
                     MARKDPAFYERVEKDKVLL"
     gene            5470..>6165
                     /locus_tag="VFF28_06105"
     CDS             5470..>6165
                     /locus_tag="VFF28_06105"
                     /inference="COORDINATES: protein motif:HMM:NF017539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HZX45228.1"
                     /translation="MWRHKLKDRDVGRFNSATQEYLESWLEKRRISFTPRQVISGTGE
                     QLSLRKVLSNTPLVCLTGGSGFGKSVLAVDAARSIAYTGTPEGKTVLYLDTTQLGPLD
                     IDSPDNLTRWACQYGSVGSKFGTDFIEKRLRSESNTCIIDGIDEVMGIEKRFRLHNWI
                     SSLTQAYHSHFCVVSSSAFITDPIRRRFEEFRVAPLDNDSITEILRPELQRNAAYSGL
                     DVQDQINTFWNYLE"
BASE COUNT         1955 a         1254 c         1388 g         1568 t
ORIGIN      
        1 tataagctag gcaaataggt gagaaaaaaa tggtaaagaa agaaaaaata atcgggattg
       61 accttggaac atcaaatagc gcagcagcag tcctccaggc aggaaaaccc actattatac
      121 caagcgcaga aggaacgacc cagtacggaa aagcatttcc ttcagttgtg gcttttacaa
      181 aggatggcca ggtgcttgtc ggagagcccg caagaaggca ggcagtgtca aaccctgaag
      241 ggacgataac cgctgcaaaa agaaagatgg gcagcaactt caagtacaat atcaacagca
      301 aggaatatac gccgcagcag atctcagcat tcatcctgca gaagataaag aaagacgctg
      361 aggaattcgt cggagagccg ataaaaaaag cagtgatcac cgttccagct tattttgacg
      421 atgaccaaag gcaggcgaca aaggatgcag gagagatagc aggccttgag gttgtaaggc
      481 tggtcaacga gcccacagca gcatctatgg catacggcct tgacaagact gacaaagacc
      541 ataagatcct tgtctttgac cttggcggtg gaacgcttga tgtgacgata atggagtttg
      601 gcgaaggagt ctttgaggtc aaatcgacaa gcggagacac ccagcttggc ggaacagaca
      661 tggatgtctc gataatgaac tacatcgccg aagacttcaa gataaaaaac gatgtcgatc
      721 taagcgacaa tgatacagca atgcaaaggc ttagggaatc agcagagaaa gcaaagatag
      781 agctaagcac agtgacagag acagacataa accttccttt ccttacagaa aaggatggga
      841 ctcccataca cttgcagatg aggataaaca gggcaaagct ggaagagctg gtcagcccca
      901 taatcaggag atgcaagcac cctctagagc aggctatcag ggatgcaaag ctgtctctat
      961 cagacatcga cagggttatt ttggttggcg gcccgacaag gatgcctatc gtaagaaaat
     1021 ttgtcgagga ctatgctgga aagaaagtgg agaggggagt agacccgatg gaatgtgtag
     1081 ctcagggagc agccatccag gcaggtatac ttgcagggga agtaaaagac atcttgctgc
     1141 tagatgtaac gcccttgaca ctgggtatag aaactcttgg aggagttaga acagagctaa
     1201 tcccaagaaa caccacagtc cctacaaaaa agagccagat attttcaaca gcatcagaca
     1261 accagccagc ggtgactatc aacgtccttc agggagaaag gccaatggcc caggacaaca
     1321 aaagtcttgg aagattcgac cttgtcggaa ttcctccggc gccaagaggc atacctcagg
     1381 tagaggtcac tttcgatatc gacgcaaacg gtatccttca cgtctccgca aaggacttag
     1441 gtactggaaa agagcaaaag atccagataa ccgcaagcca gaagctgaac agtgaagaga
     1501 ttgagaagat gaagaaggat gcagaggctc actcggaaga ggacaagaag aggcagcagg
     1561 agatagaggc gataaatcag gctgactcgc ttgtctacac cacaaagaag ctgttcaagg
     1621 actttgaagg caaagtggat tcaaaagagc ttgagacagt caaaggaaag atccttgagc
     1681 ttgaggagct gttgaagcct gaaaagaaag acagcgccgc gataaagaaa aagatggacg
     1741 aggtcaatga gcttgtccaa aagatgtcga ctgagatgta tcagaaagca gcagcagaag
     1801 cagcaaagaa aggaaaaggg caggcagggc ccgcaccagg tgacgaagaa ggtggagaag
     1861 atccgtccaa gaaacctaaa gatgacaacg ttgtcgacgc agactatgaa gtagaaggcg
     1921 ataagaaaga agaaggcaaa aagaaaggaa aatccaagtg attttacctt tttttattat
     1981 tagatttgcc acaggagaaa aacacctcca ataaacagga tgctgccaac aataatggct
     2041 tcgatggttt ctctgcggag aaacctacaa atcaccccgt aagggccgtc ccccgatttg
     2101 tcttgccatt gttggctttt acctatcttc attggtggtg tttttcctca gcaaatacca
     2161 aaacaacaaa tgccgagccg tgacaggggt tgctccctac gggatgtcac ggagccttgc
     2221 ggagcaaggc gtcgaaggca ttatttgttg ttttgacatc cataacaact ttaccaattt
     2281 atgaaaatct tagccatcaa cggcagccac agaaaaggaa acacagattt cttactcagc
     2341 aatctattgg aagatacgga cattatcaga ttaagagaga agaagataac cttctgcggc
     2401 ggaggagatg attgctgccc aaagactggt acatgctata tcaaagatga catgccagag
     2461 atagaaaaaa agctagttga ggcagacctc gtgatacttg cttcgccatg ttattttttt
     2521 aatgtcacag cgatgatgaa gaattttatg gataggtgca accctttctt cttcaacctc
     2581 aagttaaagg gaaaaaaatt cttcttgata cctttagggg gccatccaaa ttcattgcct
     2641 gatgccttgg tctgcatgaa aaattttgtg aagggaatac atggtgaggc tgtcggtgaa
     2701 tattctgcag tggctaaccg acgtggcgac atgtcaaaaa atgaaaaggt tattaaagag
     2761 cttgaggatt tgaaaagtag gataaaaaat ctctaacatg gcaaaagact actacaagac
     2821 actaggcgta ggcaagaacg cgacaaagga agatgtaaag aaagcctaca aaaagcttgc
     2881 aaggcaattt catcctgata taaataaaca agcagatgca tctgacaagt tcaaggagat
     2941 aaacgaagca gcccaggttc ttggcgacga cgaaaaacga aggcagtacg accagtttgg
     3001 cgatgcagac tcatacaaac aggcatccgg ctttagaggt tttgactcct cggactttgg
     3061 ctttggtgat tctgattttg catcttttga cttcggcgat atattcgaca aattctttgg
     3121 cggaggaggc tcgtttggcg gaagaagggg aagaagggcg caaagaggct ctgaccttag
     3181 atatgatatg gagataacac ttgaagaagc tgcctttggc gtagaaaaag agataatcat
     3241 tccaaaaaac gaaacctgtc cgacttgtgg aggcacaggt gcaaagacat ccgatgacat
     3301 aatcgactgc cctgaatgcg gcggccaggg agttgtcagg aggacgcaaa ggacaccctt
     3361 cggattattt tcaaccacta ccacctgccc aaaatgccat ggcacaggaa agttcataaa
     3421 aattgagtgt cccacatgcg acggcacagg catcgttcat gaaagaaaga agataaggtt
     3481 aaagattcct gcaggctcag aagagggcac aaaccttaga gtaggcggtg aaggagaacc
     3541 cggagcaaga ggagcgccat caggcgacct atacatagtt atccacgtaa aggagcatga
     3601 cacctttgaa agaagaggag acgatatcta tctcaggata aatattccat ttacactggc
     3661 agctctaggc ggagagcttg aagttcctac cttggaaggc aaagcaaaat taaaagtgcc
     3721 ttcaggcacc cagtcaggca ctgtctttaa gatgagggat ctaggtatcc cattcctgca
     3781 tggccatgga aaaggcgatg aactcgtcga ggtaggcatt gaagttccaa aaaaacttag
     3841 ctcaaagcag aaaaagatcc ttgaagagtt tcagaaggaa agcccaaaga aaggattttt
     3901 tgacttataa atttccaaaa atcaactgtc tagccttcca ccatttatct ttctaatata
     3961 atcaaaaacc tcttttggca ccagctcttc ccaatccttt gagcatatta tccttttccg
     4021 tatctcagta gcattgatat cagggataag ctgtatcctt ctgacttcag tcttccgctc
     4081 tagaaaaagt tttgctgtaa actcatttcc tgtatagact atgtcaaact caggcactac
     4141 cttccttaca tgctcgaccc atttctcatc attgaatata tcaggtacag gaacgatgtc
     4201 ataactcgag acattatgcg cttccaatgt atcctttatc atttctctcc tttcttcata
     4261 tgtaaacgga ttttctttgg tgccggattc ctgagagctt ccaatggcga ttatcacctt
     4321 gctgcattct tttagcgcaa gtttcacatc ggaaagatgg gctttatgaa aaggctggaa
     4381 cctgccgata aataatgcta tcttatcttg ctttaagatt gatttcatcc tctcgatgaa
     4441 ttcatcagct attaataaac ttgtctttgc gccgtctctc gtatggatat agtgataatc
     4501 tgcgtcctct ctgcttgaga cagcagcgct gaagtcattg accgctctgg actcgataac
     4561 tcctttcttg ttcaagtctt ccaatactgc actgacagca aaatgccttt tttcccgaaa
     4621 tcccagattg aataagacag ctcttgcagc atgcaacata gagtaatagc tttttattat
     4681 tgaccatttg tagtcttcat cctcaaaagc cttctcagcc tttccaaagt catagtcaga
     4741 ttccctcagt tctttttctt caattctggc atcaggctct atcctttgaa gaaatccccg
     4801 ggcaatacac tcatcaatgt tcataataac accttatcct tttctactct ctcataaaaa
     4861 gcagggtctt ttctcgccat cagcgaccat tcctgatttg aagaaacctg caccctgatc
     4921 tccttgtcca acttttttga aatattgatt atatcactca ttaggcttga tttatcaggc
     4981 gctcctatga caagaatatc tatgtcgctt gactcaatat tctcacctct tgctgcactg
     5041 ccataaagat aaattttgga attatgtttt tccgatagtt cttttatagg ctcaagttct
     5101 gataagctga atagaatttt taggtgttta acgatagtat tttcattatt gagcttgtac
     5161 aggttagtaa caccaatctt tttcattgaa ataatctttt cttcttgcat cagcttaagg
     5221 catttgatca tagttgcttt ggctatcttt atcttggcca aaagttctgt ctgtgaaaat
     5281 tccctggtct ggttgctgaa gaaaaaagag ataatcttca atactttatt atttcctata
     5341 agtctaatta attgaaccat ttggtatatt tttttagata agtatttaaa ttcttcctca
     5401 gacgttcaat tactccttag tagtaattat agaaaacctt tttaaactga caaagattct
     5461 taagcataaa tgtggcgcca taaacttaaa gatagggacg tcggaaggtt taatagtgct
     5521 acacaggaat atttggaaag ttggctagaa aaaagaagga tttcttttac ccctagacag
     5581 gtgatttccg gcactggaga acaacttagt cttcgcaaag tgcttagcaa caccccttta
     5641 gtatgtctaa caggaggatc aggctttggg aaatcagttt tagctgtaga tgctgctcga
     5701 agtattgcct acactggcac acctgaagga aaaacagtat tatacttaga tacaactcaa
     5761 ttaggcccat tggatatcga tagcccggat aaccttacta ggtgggcttg tcaatatggc
     5821 agtgttggaa gtaaatttgg cactgatttt attgaaaaaa ggcttcggtc agaatccaat
     5881 acttgtataa tagatggtat tgatgaggta atgggcatag aaaaaaggtt taggcttcat
     5941 aattggattt cttcattaac ccaggcatat cattcccatt tctgtgttgt ttcatcaagt
     6001 gcttttatta cagatcctat taggagaagg tttgaagagt ttagggttgc acccctggat
     6061 aatgattcga ttacagaaat tctgcggcca gaactgcaaa ggaatgctgc atatagcgga
     6121 ttagatgttc aggatcaaat taatactttc tggaattatc ttgaa
//
LOCUS       DASRUK010000137         2849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2130517, whole genome shotgun sequence.
ACCESSION   DASRUK010000137 DASRUK010000000
VERSION     DASRUK010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2130517"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            507..728
                     /locus_tag="VFF28_06110"
     CDS             507..728
                     /locus_tag="VFF28_06110"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HZX45229.1"
                     /translation="MAADSSRERTSLANERTTLAYVRTAIAFAAFGLFLWKFFEGPWT
                     QLLAVSFILLGIFFLTTGIVLFSIRKKGS"
     gene            728..1942
                     /locus_tag="VFF28_06115"
     CDS             728..1942
                     /locus_tag="VFF28_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZX45230.1"
                     /translation="MGTGILILGVVILVGFIGNFFFKKTRVPESVFLMLIGIALGPVL
                     GIVSGEFFVENASFLISLALVVVLLDSGLALNMAKAAKNAPKALVFTILVMLSTISVV
                     AGVTYFFFGWPLINGIFLGIIGSGTTTITITHLVERLSIGRNTKTLLILESVINDITL
                     ITSISILLMFMSTEGKSSILKVVFNELGVSFILGLAFALVWAYIFLRYIHGNKLAYVF
                     TLGMAFVVFDGVEFFGFNGAIAALVFSLALGNYSYMVGKLKKYSKILAPVKADILTIR
                     NINSEITFIMRTLFFFFLGVIFDKGAVKGGVIVFVLIIVVSEVVSRFIAAKALSLFEP
                     RFKNSVPVLTTLVASGFTSTLVAFLSIEAGIKIPNLAEIVLLLVMFTTLWAIVGTAVI
                     ERRLSKARVKTY"
     gene            2200..2544
                     /locus_tag="VFF28_06120"
     CDS             2200..2544
                     /locus_tag="VFF28_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45231.1"
                     /translation="MKTLLIFLLLSLIAGCSQDCDTLEKEIKDRLSEANYCDTAEDCT
                     FTSDFGCPFDCYVFYNKAADITDAQDRLASYKKEGCDLCVYSCIEAPDEKTIRCIDNK
                     CVDTRINSSENI"
     gene            complement(2527..>2849)
                     /locus_tag="VFF28_06125"
     CDS             complement(2527..>2849)
                     /locus_tag="VFF28_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HZX45232.1"
                     /translation="IIGGWLMLPAVAGITLIYGVLRKELALELLFILGGSRMLLDFMS
                     PLQIFIFALVVSIYIPCIGTFAVLKHEFGLKNSLLIAFFTIGLAVVIGGLVGRLFIFL
                     NILA"
BASE COUNT          808 a          602 c          587 g          852 t
ORIGIN      
        1 cagcagggca tgtatctgct gagcaagtgg ccaagtatat tagagaaaac acaagtagct
       61 attgatgtgc ttacgcacat tgtgggcatt catctacccc actaacgtgg ggagacctag
      121 cccacaaact tttagcttac agaggtaaaa cagaaaaaca aattacttgc ggaaagcagt
      181 ttccagacca cctgaaataa atgatagttg taaggcttgt ccgttgtata aaaagtgaaa
      241 agacgacacc atccaaacta tctcttctta atctcagcat caatcgcttt cagcatcttc
      301 tttaaatcag ccttagaagc cttctttacc aatttctcaa atgctttcat tttagctcct
      361 tcatcccctt agtctttagt aatctagcaa gaaagcatac ttttatactt ttaggacaga
      421 ccgagcactt ctcggacagg ggctagttgt cggacaaagt ctaaaaaagc aataattata
      481 taaaaggcaa caatcaggga tttttgatgg cagcggatag ctcaagagaa aggacaagcc
      541 ttgcaaacga gaggactacg cttgcttatg taaggacagc catcgctttt gcagcatttg
      601 gattgttcct ctggaaattc tttgagggcc cctggaccca acttttggcg gtttccttta
      661 ttttactggg cattttcttc cttactacag gcattgtttt attttccata agaaaaaaag
      721 ggtcataatg ggtacgggaa tacttatatt aggagtagtc atccttgtcg gatttatcgg
      781 taatttcttt tttaaaaaaa caagggttcc ggagtcggtc ttccttatgc taataggtat
      841 cgccctaggt ccggtgttag gtatagtctc aggtgaattc tttgttgaaa acgcatcttt
      901 tttgatatcg cttgcactag tggtggtgct tttggacagc gggcttgctt taaatatggc
      961 aaaagctgca aaaaatgccc caaaagccct ggtctttact attctggtca tgctcagcac
     1021 catatcagta gttgcaggag tcacttattt tttctttggc tggccgttga taaatggtat
     1081 attcctggga atcataggca gtggtacaac aaccataacc attactcatc ttgttgaaag
     1141 gctaagcata ggccgcaata caaaaacatt gcttatctta gaatctgtca taaatgatat
     1201 tactttgata acctccatat ctattttgct gatgtttatg tcaacagaag gtaaatcatc
     1261 gatcctcaaa gtggtgttca acgaactggg tgtttctttt atcctgggcc ttgcgtttgc
     1321 ccttgtctgg gcttatattt ttttaagata tatccacgga aacaagctcg cttatgtctt
     1381 taccctgggt atggccttcg tcgtttttga tggcgtggag tttttcggtt ttaatggagc
     1441 catcgctgca ctcgtcttca gccttgctct tggcaattac tcatatatgg tcggcaagtt
     1501 aaaaaaatat agcaagatac ttgctccagt caaagcagat atattgacga tcaggaatat
     1561 taattcagag ataacattta tcatgcgcac gcttttcttt ttcttccttg gagtgatatt
     1621 cgacaaaggg gctgtcaaag gtggcgtgat tgtctttgtt cttataatag ttgtatcaga
     1681 ggtcgtctca aggttcatcg ctgcaaaagc cctctctctt tttgagccca ggtttaagaa
     1741 ttcagttcct gtgctcacca cgcttgtagc aagcggtttt acctcaacac tggtagcatt
     1801 cttatcgata gaggctggca taaaaatccc taatcttgct gagatagtgc tgctcctggt
     1861 catgtttacc acactttggg caatcgtcgg aacagcagtc atcgaaagaa ggctctcaaa
     1921 agctagggta aagacctact gacagctgta gcaactgagt ttaatcgtaa aaattctttt
     1981 ccgtatgtat taaactcttg gcgggggcta tactgtcttc aaaactctta atttgtttct
     2041 caaaggctct gtatccccgg ctggggcgac cccctacgac gagctgtata atggttattg
     2101 gatggagttt tgaccttagg acagtagagt ccccctgctt agcttaatac atactctttt
     2161 cctataggtt tataagcatc gtttactatt gctaccccta tgaaaacact cttaattttt
     2221 ctactgctct cattaattgc tggctgcagc caggattgtg ataccctaga aaaagagata
     2281 aaagacaggc tatctgaggc caattactgc gacaccgcag aggactgtac ctttacatca
     2341 gactttggct gcccttttga ttgctatgtt ttctacaaca aggctgcaga tattaccgat
     2401 gcacaagata ggctggcatc gtacaaaaaa gaaggctgcg acttatgcgt ttacagctgc
     2461 atagaagccc ctgatgaaaa aacgataaga tgtatcgaca acaagtgtgt tgacacaagg
     2521 ataaactcaa gcgagaatat ttaggaaaat aaacaacctt ccgacaagtc cccctataac
     2581 gacagcaagg cctatcgtaa agaaggcgat aagcaggctg tttttaagtc caaactcatg
     2641 cttgagcaca gcaaatgtcc ctatgcaggg tatatatatt gacacgacaa gtgcaaagat
     2701 aaatatctgt agcgggctca tgaaatcaag cagcattctg cttccgccaa gtatgaataa
     2761 gagctccaat gccagttcct ttcgaagaac accgtaaatc aaagttatgc ctgcaacagc
     2821 aggaagcata agccatccac cgattatcg
//
LOCUS       DASRUK010000138        11392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_633342, whole genome shotgun sequence.
ACCESSION   DASRUK010000138 DASRUK010000000
VERSION     DASRUK010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 11392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11392
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633342"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..607
                     /locus_tag="VFF28_06130"
     CDS             197..607
                     /locus_tag="VFF28_06130"
                     /inference="COORDINATES: protein motif:HMM:NF003067.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit beta"
                     /protein_id="HZX45233.1"
                     /translation="MDYKELLKKAREQLPKSVFEKERFEIPKIRGHIQGNKTILTNFL
                     QVADTLRRPSEHLLKYILKELATPGDIKKNGSVIIGAKISASRINDKVRQYAIEFVLC
                     SECAKPDTKIEKEDKFTFLKCHACGAKHPIKAKI"
     gene            604..894
                     /locus_tag="VFF28_06135"
     CDS             604..894
                     /locus_tag="VFF28_06135"
                     /inference="COORDINATES: protein motif:HMM:NF016157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF424 domain-containing protein"
                     /protein_id="HZX45234.1"
                     /translation="MIIKIHRAHEGRKLVALCDNELIGKVFEEGDAFLDLRLKFYAGE
                     EKGRDEVLEALKNASIVNAVGKESVGLLVSAKIVEEKCILKVNGIPHAQAVI"
     gene            complement(891..4427)
                     /locus_tag="VFF28_06140"
     CDS             complement(891..4427)
                     /locus_tag="VFF28_06140"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF003103.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II large subunit"
                     /protein_id="HZX45235.1"
                     /translation="MVMIATAEMQKYFDEIEKKVALAYKEARKARAKGFDPELDVALP
                     LAKNMAERVEGLISTVAPEVENSGLSQRIKELEKQFGDLDWRVAMTISLEVAQQKICK
                     FSSILRAMETGIRVGIAYLTLGIVASPIEGFVELKLKKTRDGKEYFALMYSGPIRSAG
                     GTAAAVSVIVSDYVRKQMGYAPYDPTDDEVNRLVTELYDYHERINNLQYLPSEEEIRI
                     LIKNLPVQIDGDPSEEIEVSQYKDLPRIETNRIRSGPCLVIGEGIAQKAPKLWKKLGK
                     WGKEFGLDEWMFIEEFIKKQKKIKAKSAVTEDTAKVTPNYTYINDLVAGRPVLTHPLR
                     PGGFRLRYGRSRASGYSSATIHPALTYILKGYIAVGTQLKVERPGKAASLSCCDSIEG
                     PIVKLNNGDVMRIDTIPQAKKVQDEIKEILFLGDILFSYGDFFNRAHILLPPGYCEEW
                     WALELEKAIVDMFGNLDIDKCSELAEIKSSELSAILKDPMKVRPTAAVSLQLSRKISI
                     PLHPAYTYHWNLLSAQQFKDMMSWLGSANITHEGENLIKVIMPHDENNKRSLELIGLP
                     HRFVNNEFVVIEKDDALAVYDIFSLAEKKRLAEMACQMDDTKKPLDIINQNSPIRIRD
                     KSGYFIGARMGRPEKAKMRKLAGSPHVLFPVGDEGGKLRCFQSAMEKGKVSAEFPLYK
                     CPKCSAISIFSVCETCQVPSKKMFYCKECGVLESAECKHGPAKTYAKQDLDINSILEK
                     LKVHLRESSLPDLIKGVRGTSNKDHIPEHLAKGILRAKHDVYVNKDGTTRYDMTQLAI
                     THFKQKEIGTSIERLRELGYIKDIHGADLVDPNQVLELKCQDVILPACDDSPDEGADK
                     VLFRVANFIDELLVKFYGCKPFYNLKSPKDIAGSLVIGLAPHTSAAITARIIGFSKTM
                     GLYAHPMFHAATRRDCDGDEASVTLLMDVLLNFSRQFLPNNRGATQDAPLVLTSMLIP
                     SEVDDMVFDLDIVSRYPLEFYQACMQYKQPWDVEIEQLGKRLNKETQYEGLGFTHDIE
                     DINDGIKCSAYKTLPSMEDKLKGQMELAEKIRAVDTSDVARLVIEKHFIRDIRGNLRK
                     FSQQIFRCVNCNEKYRRPPLVGKCVKCGGKLVFTISEGSIVKYLEPAISLAEKYNVPP
                     YLKQSLELAKRRVEGIFGKDKEKQSGLGQWFG"
     gene            complement(4438..6006)
                     /locus_tag="VFF28_06145"
     CDS             complement(4438..6006)
                     /locus_tag="VFF28_06145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF003118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase II small subunit"
                     /protein_id="HZX45236.1"
                     /translation="MEQTVIQKKKAVEFFLKKEILVSSDLLAGIDDNSLDCLSRGMMG
                     NEGNFLFLNNDLSEAVQKGQHRGINWIELESSKAFLERGKDNKVYSEFLAILNTVKQK
                     PAYEKVKVIFSHDEDSKKRDIQDFVKYFNARYQALEKILKQRPDLRHVTAISRIKNKK
                     DREEISLIGIVSDKQLTKNKNLVLTLEDPTGSINVIVNKNKPDVFEEAKSIVHDEVIA
                     VTGVNGQNILFANKIYWPEVITKEIKKCKDECYALFLSDIHVGSKNFLPESFNKFLAW
                     LNGTTGSESQKELASKVKYIFIAGDLVDGVGVYPNQDKELEIKDIYGQYEECARLLGQ
                     IPKNISVIICPGNHDAIRGAEPQPALYADFAKAIYQIPNILCLSNPCFVNIHSSEDFS
                     GFDVLLYHGYSFDYFVANVDAIRNNGGYNRADLIMKFLLKRRHLAPTHTSTLYVPEET
                     QDPLVIGKVPDFFVTGHVHKSIAANYRNITLISGSCWQSKTSFQEKVGHNPEPGRVPM
                     VNLKTREIKILKFL"
     gene            complement(6039..7250)
                     /locus_tag="VFF28_06150"
     CDS             complement(6039..7250)
                     /locus_tag="VFF28_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORC1-type DNA replication protein"
                     /protein_id="HZX45237.1"
                     /translation="MKKETLANFFEDFLAKDSLFLDKEALQSGYMPETISHRDSEIDQ
                     VAKILAPCLKLEKPSNLFIYGKTGTGKTLTVKHVANQLSSIASQKNIPLKIIYLNCKM
                     KKIADTEYRLVAQLIRELGQEIAPTGLPTDEVYKAFFNAIDSKNQLILLVLDEIDQLV
                     QKTGDEILYNLVRINEELKQSKIVITGISNDLIFIDHLDPRVRSSLSEEEMVFAPYNA
                     LQIQEILKQRAKLAFNENSLSDGVIEKCAAYSAREHGDARRALELLRVAAEVAEREGQ
                     KKVEIHHIDSAEDKIEKDRVLDTIVHQPKQSQAVLYSAIYLYANRKDNIFTGDIYDIY
                     KNISSQVGLRPLTQRRVSDIIAEMDMLGIINAKVISKGRHGRTREISVSIPQGILQKA
                     KKALEEGLALS"
     gene            7594..8277
                     /locus_tag="VFF28_06155"
     CDS             7594..8277
                     /locus_tag="VFF28_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45238.1"
                     /translation="MDDKTLSNYIRKMLSLGYPSEVIEKSLVRQGINASDIQKIISSF
                     SKPKQHFFISHPNPKHSIIALVFFAAATIMLHIFQEEGPFLAIAVSSLFYVLLYFLFM
                     LLFCGAFLFLLNLVTKRLGILSIKTTAVEFFGCLLPYFALSSLTGIRTYREITIFNLY
                     VGPMLFNLFFILSLVWFGRRLSQLYHLNLLKSTIYVLTIFFFLLTLLLILFFDAAELT
                     IFSYSFQKF"
     gene            8386..8529
                     /locus_tag="VFF28_06160"
     CDS             8386..8529
                     /locus_tag="VFF28_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45239.1"
                     /translation="MTRINIEIDDELHKKLKLNALLQNKTLIGYIGDALTEKVKEDEK
                     KKK"
     gene            8510..11044
                     /locus_tag="VFF28_06165"
     CDS             8510..11044
                     /locus_tag="VFF28_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45240.1"
                     /translation="MKKRRNSLLGKTKIAALILVGVLLIQGGCKKQVNIQEYISVSCI
                     FNSEHSKDAGKCENIIDGNPYTYWGIEDSDDVGYYEILIELIDPSKPRQPTSRPNAVT
                     RTVPASLLGLSWFKTDLDFEAMTEAPTNLDLKRIEATTFVGGSKFLKSNSTLDIRVFV
                     DGKFQSVLLKENWTGERKAIARGDFEEVSKLSITHSINPEQKDGKAYIYELIVSDQCE
                     CSAAEGECCDGCQYKDTNIKCGDTEPANTCREQAYCTGESADCPTPQIKSKGTVCRLN
                     VSSCDIAETCDGEDPTCPPVDKFKDKGEACGMCRECDGAAACEIKEGKKDCNGACTEC
                     DPFGACLPSGFYTYRNGEIIDKSDTACCTEGASCVYNGRCNLTKSYVQTDGNLRQCLL
                     GNWSESKIEKKDWENKTGYCPVDDCYYSSECFKEGEFLLSGGDHYCEKGAWTTRTKLL
                     ATQLLNIVSADDTYIDDYTLFCDSYKDTLNYYNYLVKGKELTKYIEGKANNFCVLELG
                     KDKSLKAEEGQVIVGISLKDDVNKEFIQTLEGVGKCPSYGDGKFHQCDTPKAWYENLT
                     KSVIYSDKSLIGIEGFEANAWESFLTFLREPFTFIMRVVQRILANQETLELEVSDFSK
                     LYIDKKGDKLIQGVAEKKYNGEFFTVKYSLFEEDICSSIEKSFAQFSQGEQIVQCYRE
                     ELEGAYYAVSTSPNAMGLWTELTSKLRTPPPVRYAVPYCQFKQSCSADENTVLLASGV
                     YDAHAAMEGGFEYKVCCEEFKKAETGERLITLSEPSDAHTSEEGFDYQVFIEGLKGDA
                     ECNVISTKCSQGETCILSMSERHDGHVSQCDNQGFGIKVCCKEAGG"
     gene            11046..>11392
                     /locus_tag="VFF28_06170"
     CDS             11046..>11392
                     /locus_tag="VFF28_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45241.1"
                     /translation="MKYKTILAILILLTAGVVIAYAAYDVKQPSHSTLYTNNILAKDA
                     GDINLVGNFIVDGELKLKSGQPIGAGYWAKANSDISYTEGSVGIGTATPSGKLHIIGS
                     GTSGLDPIIKITG"
BASE COUNT         3215 a         2427 c         2408 g         3342 t
ORIGIN      
        1 ccatccataa ttttgtcaat ccaaatatgg aacttaaagg caggacgcct gccgaggctg
       61 caggcatcaa acttgacttg ggaagaaata agctgctgaa attgatagag atttcatgta
      121 tgccgaagag ttaacagtgc ctggttacct gcgtttaaaa agataatttt atatattgcg
      181 gaaggacacc agacgtatgg attacaaaga actgctaaaa aaagcacggg aacagctgcc
      241 taaatctgtg tttgaaaagg aaagatttga aattccaaag attagggggc atatccaagg
      301 caacaagacc atacttacca attttctgca ggtcgctgac acgttaagaa ggccaagcga
      361 gcacctgcta aagtatatcc tgaaggaact tgccactcca ggcgatatca agaaaaacgg
      421 ctcggtgata atcggggcaa aaataagcgc aagcaggata aacgacaaag taaggcagta
      481 tgccatcgaa tttgtcctgt gcagtgaatg tgctaagcct gatacaaaga tagagaaaga
      541 agataagttc accttcctta aatgccatgc ctgcggagca aagcatccta tcaaggcaaa
      601 gatatgataa ttaagatcca cagggcgcat gaagggagaa agctcgttgc actttgcgat
      661 aatgaactta tcggaaaggt ctttgaggaa ggggacgcat ttcttgacct aagattaaaa
      721 ttctatgctg gcgaggaaaa gggaagagat gaggttcttg aggcattaaa aaatgcttcg
      781 atagtcaatg ccgtgggcaa agaaagtgta ggtcttcttg tcagcgcaaa aattgtcgaa
      841 gagaaatgta tcctcaaggt taacggcatc ccacatgccc aggcggtaat ctagccgaac
      901 cattgaccaa ggcctgactg cttttccttg tcttttccaa aaataccctc taccctgcgt
      961 ttggcaagct caaggctttg cttaaggtag ggtggaacat tgtatttctc tgcgaggctt
     1021 atcgccggct caagatactt tacaatcgag ccttctgaga tagtaaacac caatttgccc
     1081 ccgcacttga cgcatttacc cacaagaggg ggccgtctgt atttttcatt gcagttgacg
     1141 cacctaaata tctgttgaga aaactttctt aggtttcccc taatatctct tataaagtgt
     1201 ttttctataa caagtcttgc cacgtctgat gtatccactg cccgaatctt ttctgcaagc
     1261 tccatctggc ctttaagctt atcttccata gaagggaggg tcttgtatgc agagcacttg
     1321 atcccatcat tgatatcttc tatgtcatgg gtaaatccta ggccttcgta ttgcgtttcc
     1381 ttgttgagcc tttttcccag ctgctctatc tctacatccc atggctgctt atattgcatg
     1441 catgcctggt aaaattcaag aggatacctg gatactatgt ccaaatcaaa taccatatcg
     1501 tccacctctg agggaatgag catcgaggta agaacaagcg gagcatcctg ggtcgctccc
     1561 cggttatttg gcaaaaactg ccttgagaaa tttaagagca catccataag aagagtgact
     1621 gaagcttcgt caccgtcaca atcccttctt gtcgctgcat gaaacattgg atgagcatac
     1681 aaccccatgg tctttgaaaa accgattatt cttgcagtga tagcagcaga tgtatggggg
     1741 gcaagaccta tgacaagcga tcctgcaata tccttggggg atttcaggtt atagaaaggc
     1801 ttgcagccat agaatttaac caaaagctcg tcaataaaat tggcgaccct gaagagcacc
     1861 ttgtctgcgc cttcatctgg tgaatcatca catgccggca ggattacatc ctgacacttt
     1921 aactcaagta cctgatttgg gtcgacaagg tctgcgccgt gaatatcttt tatataaccc
     1981 agttctcgca acctttctat gcttgtgcct atctcctttt gcttgaagtg ggtgattgca
     2041 agctgagtca tatcgtacct tgtcgtgccg tctttattta cataaacatc atgttttgcc
     2101 ctgagaatcc cctttgccag atgctcgggg atgtgatcct tatttgacgt tcctctcact
     2161 cctttgataa ggtcaggaag acttgattcc ctgagatgca cttttagctt ttccaagatg
     2221 gaattgatat ctagatcctg ctttgcatag gttttggcgg ggccgtgctt gcattcggcg
     2281 ctttccagca caccgcactc cttgcagtag aacatttttt ttgaaggaac ctgacatgtt
     2341 tcacatacgc taaagatgct tatcgcgctg cacttggggc acttgtaaag cggaaactcg
     2401 gcagatacct tccctttttc catcgcagac tgaaagcatc gaagcttgcc tccttcatct
     2461 ccgacaggaa acagtacatg gggcgagcct gcaagcttcc tcatctttgc tttctcgggc
     2521 ctgcccatcc ttgccccgat aaaatatcct gacttgtccc ttatccggat aggtgaattt
     2581 tggttgatga tgtcaagggg ttttttagta tcatccatct ggcacgccat ctctgcaagc
     2641 cgcttttttt ccgccaaaga aaagatgtca tagaccgcca aggcgtcgtc tttttctatg
     2701 acaacgaact cgttgtttac aaacctatgc ggaaggccta tcaattccaa agaccgctta
     2761 ttgttttcat catgaggcat tattaccttg atgagatttt ctccctcatg agttatattt
     2821 gccgagccca gccatgacat catatcctta aactgctgag cggataataa attccaatgg
     2881 taagtatatg ccggatgcag agggatgctt atttttcttg acaactgaag agaaactgct
     2941 gcagtcggac gaaccttcat gggatccttt aatatggctg aaagctcgct tgatttgatc
     3001 tctgcaagct ctgaacattt atctatgtcc agattgccaa acatatcgac aatggccttc
     3061 tcaagctcaa gcgcccacca ttcctcacag tagccaggtg gaagaaggat gtgtgctctg
     3121 ttgaaaaaat cgccgtaact gaacaatatg tcacctagaa atagaatttc ctttatctcg
     3181 tcctggacct tttttgcctg agggatagta tctatgcgca tgacatctcc attgttcaac
     3241 ttgactatgg ggccctcgat gctgtcgcag caggataaag atgctgcctt tccgggcctc
     3301 tcgaccttga gttgtgttcc cactgcgatg tatcctttaa ggatgtaggt gagtgcagga
     3361 tgtatcgtcg ctgaagaata ccccgatgct ctggaacggc catacctcag cctgaagcct
     3421 cctggcctta atgggtgagt gagcacgggt cttccggcga ctagatcatt tatataagta
     3481 taattggggg taacttttgc agtatcttct gttacagcgc tttttgcctt tatcttcttc
     3541 tgttttttga tgaattcctc gatgaacatc cactcatcaa gcccaaattc ctttccccat
     3601 tttcctagct tcttccaaag cttaggggct ttctgggcaa ttccctctcc aatgaccagg
     3661 catggtcctg agcgtatcct atttgtctct atcctgggca agtccttata ctgggaaacc
     3721 tcgatctctt ctgagggatc tccatctatc tgcacaggca gatttttgat aagtatcctt
     3781 atctcctctt cgcttggcag atattgaagg ttgtttatcc tctcatgata atcataaagc
     3841 tcggtaacca gccggtttac ttcatcatcg gttggatcat atggggcata acccatctgc
     3901 tttctgacat aatccgagac aatcactgaa actgctgcgg ctgtcccccc tgcagatctt
     3961 atagggcctg agtacatcaa ggcaaaatat tccttgccgt cccttgtttt cttcagcttt
     4021 aattctacaa aaccttctat tggagaagcg acaattccaa gagtaagata tgctatgcct
     4081 acccttatcc ctgtttccat agctctcaat attgaactga atttgcatat cttttgctgc
     4141 gctacctcaa gagatattgt catcgcaaca cgccaatcaa ggtcaccaaa ctgcttctca
     4201 agctctttga tcctttgaga tagccctgaa ttttcaactt caggagcgac ggtcgatatc
     4261 agcccttcta ccctttctgc catattcttt gccagaggca gtgcgacatc aagctcagga
     4321 tcaaacccct ttgcccgtgc ttttcttgct tccttgtagg cgagggctac ttttttttct
     4381 atctcatcaa agtatttctg catttccgct gttgctatca tcaccatttt aggttaccta
     4441 taggaatttc agtatcttta tctctcttgt cttcagatta accatcggaa cccgccctgg
     4501 ttcaggatta tggcctactt tttcctggaa ggacgtcttt gactgccagc aagagccgga
     4561 gataagcgtg atgttgcggt agtttgctgc gatggattta tggacatggc cggttacaaa
     4621 aaaatcagga acttttccta tgaccagagg atcctgcgtc tcttcaggca cataaagggt
     4681 agaggtatgg gtgggagcaa gatgccttct ttttaacagg aacttcatga taaggtcggc
     4741 ccggttatac cctccattat tccttatggc gtcaacgttt gccacaaaat aatcaaatga
     4801 atatccatga taaagaagga cgtcaaaccc ggaaaaatcc tcggatgaat ggatatttac
     4861 aaaacaagga tttgaaaggc agaggatatt tggaatctga taaatcgctt tagcaaaatc
     4921 cgcatatagt gcaggctgag gctctgcacc ccttatcgca tcatgattgc ctgggcatat
     4981 tatcactgaa atattttttg ggatctgacc aagaagcctt gcacattctt cgtattgtcc
     5041 gtagatgtct tttatctcca gttccttgtc ctgattgggg tatacaccaa ccccatcgac
     5101 aaggtctcct gcgataaata tgtattttac ctttgaagca agttccttct gggattcgct
     5161 gccagtcgtc ccatttagcc atgccagaaa tttattgaat gattctggaa gaaaattctt
     5221 tgagcccaca tggatatctg aaaggaacag agcataacat tcatccttgc atttctttat
     5281 ctcttttgtg atgacctctg gccaatatat cttgtttgca aaaaggatat tttggccgtt
     5341 tacacctgtg actgcgataa cttcatcgtg cactattgat ttggcttctt caaagacgtc
     5401 cggcttattc ttattgacta tcacattaat agaccctgtc gggtcttcaa gtgtgagcac
     5461 aaggtttttg ttcttggtca actgcttatc tgaaactatg ccgataagcg atatctcttc
     5521 cctgtctttc ttgttcttta tcctgcttat tgccgtgacg tgcctcaaat caggccgttg
     5581 ttttaggatc ttttccaatg cttgatatct tgcgttaaaa tatttcacaa agtcctggat
     5641 atctcttttc tttgaatctt catcgtgaga aaatatgacc ttaactttct cgtaagcggg
     5701 tttttgcttt actgtattga ggatagccag aaactcgcta tagaccttgt tgtccttgcc
     5761 cctctcaagg aatgcctttg aactttcaag ctctatccaa tttattcccc tatgctggcc
     5821 tttttgcact gcttctgaca gatcgttatt tagaaataga aagttaccct cattacccat
     5881 catgcctctg gacaggcagt ccagagagtt atcatctata cctgcaagca aatcagaact
     5941 taccaatatc tcctttttga ggaaaaattc tacagctttc tttttttgta tgactgtttg
     6001 ttccattttt taggtaagaa ggattatgct tagccttgct aagataacgc taatccttcc
     6061 tctaatgctt tctttgcttt ctgcaagatg ccttgaggga tagatacgga aatctccctt
     6121 gtcctgccgt gccttccttt ggaaattacc ttggcattta ttatgccaag catgtccatc
     6181 tctgcaatta tatcagatac tcttctttgg gtaagaggcc ttaggcctac ctgcgaagag
     6241 atgtttttgt atatatcata aatatctccg gtaaaaatat tgtctttcct gttggcatac
     6301 aggtagatgg ccgagtaaag gaccgcttgt gattgctttg gctggtgaac tatagtatct
     6361 aataccctat ccttttctat cttatcttcg gcgctgtcta tatgatgaat ctctactttc
     6421 ttttgacctt cacgctcagc tacctctgct gctaccctta gaagctcaag agcccttctt
     6481 gcatcaccgt gctctctggc agaataggct gcacattttt ctatgactcc atctgaaagg
     6541 gagttttcat tgaaagccag ctttgccctt tgttttaaaa tctcctggat ctgcaaagca
     6601 ttgtaaggtg caaataccat ctcttcttct gataatgaag acctaaccct tggatcgaga
     6661 tggtcaataa atatcagatc gtttgaaata cctgtgatca ctatcttgga ttgttttagc
     6721 tcttcgttga tcctgacaag attatagagt atttcgtcgc ctgtcttttg aaccagctgg
     6781 tctatctcat ctagtacaag gagaataagc tggttttttg agtctattgc gttaaagaat
     6841 gccttgtaga cctcatctgt aggaaggcct gtaggggcta tttcctgacc aagctccctt
     6901 atcaactgag ctaccaacct gtactcagta tctgctatct ttttcatctt gcaattgagg
     6961 tagattatct taagggggat gtttttttgt gaagctatgc ttgataactg gttggccaca
     7021 tgcttgacgg taagtgtttt gcctgtcccg gtcttaccat agataaatag attggagggc
     7081 ttctcgagct tcaaacatgg agcaagtatc tttgccacct gatctatctc tgagtcgcgg
     7141 tgagagattg tttcaggcat ataaccactc tgtaaggcct ctttatctaa aaataggctg
     7201 tctttagcca gaaagtcctc aaaaaagtta gcaagcgtct cttttttcat ttagtttcca
     7261 taggaaatgg aggtttataa agtttttgtg agggaagatt gaccccttca tttcgcttgg
     7321 aaagtttatt ttaaatgtga tggtgtttga ttatattata tatatattat tttctatata
     7381 gataatataa taatataata ataatataat aaaaaaataa ataaaaaatc aaaaaaaagt
     7441 aaaaaggaag aaaaaaccac aaaccagagg gtcacagtac ccattaagtt gatttccact
     7501 ggaaatcaag gggtcatacc atttacacca atgactatga ccattaacct gttaatacaa
     7561 acttttatta atcatttatc taaaaaccat tgcatggatg ataaaacact atctaactat
     7621 atcaggaaga tgctttcttt aggttatcct tcagaggtca tcgagaagtc tcttgtacgc
     7681 caggggatca acgcctcaga tatccagaag ataatatcct ccttttctaa gccaaaacaa
     7741 cattttttca tatctcatcc aaaccctaaa cattccataa tcgcactggt gttttttgca
     7801 gctgccacca tcatgttgca tatatttcag gaggaaggac catttcttgc aatagctgta
     7861 agctccctct tctatgtttt attatacttc ctttttatgc ttcttttttg cggggcattt
     7921 ctatttttac taaatcttgt taccaagagg ttggggatat tatctatcaa aaccactgca
     7981 gtggaatttt ttggctgcct ccttccttat tttgcactga gttcgctgac aggaatccgg
     8041 acttataggg agataactat attcaatcta tatgtcggcc ctatgctttt caaccttttt
     8101 tttatactct cccttgtctg gtttggcagg agattatccc aattatatca cctcaactta
     8161 ttaaaaagca ccatttatgt gcttactatc ttcttttttc ttttaaccct gctattaatc
     8221 ctcttttttg atgctgccga gttaaccatc tttagctatt cttttcaaaa attttagaaa
     8281 agtttatata agaagggctg attattaggt atattagcta agtatatgtt taagatacgt
     8341 taaataacgg acataagtaa tataatagta ggtaattaag ttaatatgac gcggatcaat
     8401 attgagatcg atgatgagct gcacaaaaag ctcaaactaa atgcacttct tcagaataaa
     8461 acgctgattg gttatatagg cgacgctctg actgaaaagg tcaaagaaga tgaaaaaaag
     8521 aagaaatagc ctactgggaa aaacgaaaat tgcagcgctt atacttgtag gtgtgttatt
     8581 aatccaggga ggatgcaaga aacaagtaaa catccaggag tatatctcag tcagttgcat
     8641 attcaactct gagcactcca aggatgcagg aaaatgtgaa aacattatcg atggtaatcc
     8701 atacacatat tgggggatag aggattctga cgatgttggg tactatgaaa ttcttattga
     8761 attaatcgac ccaagcaaac ctcggcaacc tacatcaaga ccaaatgccg taacaaggac
     8821 cgttcctgca agcttactgg ggctttcatg gtttaaaaca gaccttgatt ttgaggccat
     8881 gactgaggcg ccaacaaacc tagacctgaa aaggatagag gcgacaacct ttgtcggagg
     8941 ctcaaaattt ttgaaatcca acagcacact cgacatacgc gtctttgtcg acggcaaatt
     9001 tcaaagcgtg ctgctgaaag aaaactggac aggtgagaga aaagcaatag caaggggcga
     9061 ttttgaggag gtaagcaagc tgagcatcac ccattcaata aaccccgaac aaaaagacgg
     9121 caaggcatat atctatgagc tgatagtatc tgaccaatgc gaatgctctg cagcagaggg
     9181 agaatgctgc gacggctgcc agtataaaga cactaatata aagtgcggtg atacagagcc
     9241 ggcaaatacc tgcagagagc aggcatactg cacaggagaa tctgcagatt gcccaacgcc
     9301 tcagataaag agcaaaggca ccgtatgcag attaaatgtt agtagttgtg atatagctga
     9361 aacctgcgat ggagaagacc ctacctgccc tccagtagat aaatttaaag acaaaggtga
     9421 agcatgcggc atgtgcaggg aatgcgacgg agcagcagca tgcgagataa aggaaggcaa
     9481 gaaagactgc aacggcgctt gcacggagtg cgaccccttt ggtgcttgcc tgccaagcgg
     9541 tttttatacc tataggaatg gagaaattat cgataagtct gatactgcat gctgtactga
     9601 aggagcaagc tgcgtataca atggtagatg caaccttacc aaatcctatg tccaaacaga
     9661 cggaaaccta agacagtgct tactggggaa ttggagtgag tcaaaaatcg aaaagaaaga
     9721 ctgggaaaat aagacaggat attgccctgt agatgattgt tattatagca gtgagtgctt
     9781 taaagaagga gagtttctgc tgtcaggagg agaccattat tgtgaaaaag gggcatggac
     9841 aacaaggaca aaattgcttg caacacaact gctaaatata gtctctgcag acgatacata
     9901 tatcgatgat tatacattat tttgcgacag ctacaaagac acgctgaact actataatta
     9961 ccttgtgaaa ggaaaagaac tcacaaaata tatagagggt aaggcgaata atttctgcgt
    10021 gctagaactt ggcaaggaca agtctcttaa agctgaggaa ggccaggtca ttgttggtat
    10081 ttcgttaaaa gacgatgtca ataaagaatt catccagact ttagagggag ttggcaaatg
    10141 cccgagttat ggggacggca aatttcatca atgcgacaca ccaaaagcgt ggtatgaaaa
    10201 cctcacaaag agcgtcatct atagcgacaa atcactgatc ggaatagagg gttttgaggc
    10261 aaacgcatgg gagtcattcc tgacattttt aagagagccg tttactttta taatgcgagt
    10321 agtccaaagg atccttgcca accaagaaac acttgagtta gaggtgagtg atttttccaa
    10381 gctctatata gataaaaaag gagataagct tatccaggga gttgcagaga aaaagtacaa
    10441 tggagaattt tttactgtca aatacagcct ttttgaggaa gatatctgct caagcataga
    10501 aaaaagtttt gcccaattca gtcaaggtga acagatagtt caatgctatc gtgaggagtt
    10561 agaaggggca tattatgctg tcagcacatc gccaaatgct atggggcttt ggacagagct
    10621 tacctccaag ctcaggaccc ccccacctgt gcgctatgcg gtcccttatt gccagtttaa
    10681 gcaaagttgt agtgcagatg aaaacacagt gcttcttgct tctggcgtat atgatgcaca
    10741 tgctgcaatg gagggcgggt ttgagtacaa ggtatgctgt gaagagttca agaaagctga
    10801 aacaggggag aggttgatca ctttatctga accttctgat gcacacacaa gcgaagaggg
    10861 ttttgactat caggtcttca tagagggcct gaaaggagat gcagaatgca atgtgataag
    10921 cacaaagtgc agccaagggg aaacatgcat tttaagcatg tcagagcgtc acgatgggca
    10981 tgtatcgcaa tgtgataatc aaggatttgg cataaaggtt tgctgcaagg aggcaggagg
    11041 gtaaaatgaa atacaagaca attttggcga tattaatttt gcttacggca ggtgttgtta
    11101 tagcctacgc ggcgtatgat gtgaaacaac catcacattc aacgctgtat acaaacaata
    11161 tattggctaa ggatgcgggg gacataaacc tcgtcggaaa ttttatcgtt gacggcgagc
    11221 ttaagctaaa aagcggacag cccataggcg cagggtattg ggcaaaagca aattctgata
    11281 tatcttatac agagggcagt gttggaatag ggacagccac cccctcagga aagcttcaca
    11341 taatcggttc aggcacttca gggctggatc ccataatcaa gataaccggg aa
//
LOCUS       DASRUK010000139         9221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_633974, whole genome shotgun sequence.
ACCESSION   DASRUK010000139 DASRUK010000000
VERSION     DASRUK010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 9221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9221
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633974"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..419)
                     /locus_tag="VFF28_06175"
     CDS             complement(87..419)
                     /locus_tag="VFF28_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45242.1"
                     /translation="MVKKEIYWKNPELEKQKVRAFYKKNREKILSKLRRYPKPEDRPM
                     AKCERCGDLFKPLSNFHPNERFCLGCKLERKRERNRDRRPKTYKDGWDTIKFLSRNPH
                     HLWLGMNA"
     gene            complement(457..529)
                     /locus_tag="VFF28_06180"
     tRNA            complement(457..529)
                     /locus_tag="VFF28_06180"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(493..495),aa:Arg,seq:gcg)
     gene            complement(574..735)
                     /locus_tag="VFF28_06185"
     CDS             complement(574..735)
                     /locus_tag="VFF28_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HZX45243.1"
                     /translation="MSNNKSMLAEVWDFLKVRKAWWLAPIIIMLVLVGALIIFGQSSA
                     LSPFIYALF"
     gene            complement(767..2521)
                     /locus_tag="VFF28_06190"
     CDS             complement(767..2521)
                     /locus_tag="VFF28_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HZX45244.1"
                     /translation="MYILGISCYYHDSSAALLKDGVVVCAAQEERFTRKKHDTSFPKE
                     AINYCISSQKITINEIDYIGFYEKPLIKFERVLSQHLEMFPRSLKTFLSSTPSWINEK
                     LRVMKAIKKLKYKKDVLFIEHHLAHAAGSFLLSPFKKSAIVTIDGVGEWTTTSYGIGE
                     DSDITLKKEIRFPSSLGLFYSTITAYLGFTVNNSEYKVMGLCPYGEMDKEKNPYYLKL
                     IQVIDLKEDGSFRLDMSYFKYHFAGRMPSRKLCTLLDGPIRRPEEEITKRHKDIAAAL
                     QLIYEEALFKILNHVHKETCCDSLVIAGGCGLNSVANGKILRNTKFKNFWAQPDPGDG
                     GTSVGVASYIYHSILGKERKKLATAYLGPSYTDADIKGFLDKNKITYTQFKDEYELIK
                     KTAALLHTNNVVGWFQLGMEWGPRALGARSILANPANPHAKELLNTKVKHREKFRPFA
                     PVVCDDDAKHYFDTDIPIPEPTDYMLMVYPIKEKWHSKIPSVTHVDGSGRLQTVKRSD
                     NSLYYDVIKEFGRLSSIPILINTSFNIRGEPIVCSPYDAYKCMMGTGIDCLVIGNFLV
                     KRDDNIKDAWDSEKLAND"
     gene            complement(2529..2762)
                     /locus_tag="VFF28_06195"
     CDS             complement(2529..2762)
                     /locus_tag="VFF28_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45245.1"
                     /translation="MSFPKGFKKGMHSFGTNLSVIINSVLLTIVYFLGVGLTAIIAKL
                     FGKHFMDMEKKKESYWSDLNLGKKPIESHYRQF"
     gene            complement(2759..2959)
                     /locus_tag="VFF28_06200"
     CDS             complement(2759..2959)
                     /locus_tag="VFF28_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45246.1"
                     /translation="MQFDDGGLTEKIGIGSGFIFSYLFFTCIIFLILVYANKTENFAL
                     LKAFSISAPVLLAGIMTKRLLK"
     gene            complement(2999..4519)
                     /locus_tag="VFF28_06205"
     CDS             complement(2999..4519)
                     /locus_tag="VFF28_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HZX45247.1"
                     /translation="MEQNPAIDLALDTINLGKQAIIFVNTKRSAEKTAEDISKKIRGI
                     DLQEISDKSLHALTKPTKQCERLSICLRKGIAFHHAGLVAKQRELIEENFKKGAIKII
                     CATPTLAYGLNLPAFRSIVKDPKRYGHRGLDYLPVLEIHQMWGRAGRPGFDRFGEAII
                     IAQSENEKEELLERYINGKPENIYSKLAVEPVLRTYILSLIASGFVNTKQQLFDFFSK
                     TFWAHQFGDMYELNQMLQKMIGLLQEWEFIMGNKDEFQGADELGDERYRPTIIGKRVA
                     ELYIDPLTAKKFIDCIKKAASKETNVFSFVQMISHTLELRPLLKVKAKEYDKVQEVLT
                     KNFTFLLEEEPNIYDQDYDDFLNSIKLAMFFQEWLDEKDEEYLLETYDIRPGEIKVKL
                     DIADWLLYTSEEMARLMQFQPLLKDIAKLRVRLKSGVREELIPLLNLRNIGRVRARKL
                     FNNRIKDLGDVKSADITKLSQILGKNIAIGIKEQVGEKVEPVKENKRKGQISLSDY"
     gene            complement(4569..6434)
                     /locus_tag="VFF28_06210"
     CDS             complement(4569..6434)
                     /locus_tag="VFF28_06210"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HZX45248.1"
                     /translation="MVVLVLCISIAAAVKTLTVYETEPVSLRPQATDADKDSITYSYS
                     PPLDSDGKWQTGYGDQGEYIITINASDGEAVTTKDVLLVVKKKNIPPVIDSAKPGTIN
                     LEVEETGKLTFGIKASDINKDSLTYSWKIDDKEAAWGQSFEYSPYYGDAGTHKIKAVI
                     SDGDVTIDKAWQVSVKKVDRESAIFSSFKDIEAEEGKVLQLDLPDFKKYNLDYELSDP
                     LGQDNYWQIGYDEAGIYNIALTIKDGKYISTKKIKVVVKNIDRPLSFKPIANSWITEG
                     DKITIALEADDPDGEKIKYSAQNLPEGAALKDNVFEWQTSPATVSKDSLVKKALDNLH
                     LLYTPYKITFTASADSEEKQSATIFIKNINQPPVLETIEPIIVDEGEEINIEPKAADP
                     DGDKLSYSYRGWFDVNRYKTTFDDAGNYKVSIIASDGFLTDEKTVDITVNDINRPPQF
                     SPMQAVEISEAQEIKIPLLIFDPEEDPIEFIKDSLPKNAAIKEGKLIYTPDYDTVKEG
                     STLLTINLKATDGTNNITQPIDITVHNKNRPPKITGASKVTAAKKGEKAIFKIIAEDP
                     DGDDLAYTWQFGLFDKRELGPSISRTFKTAGTKKITVFVSDGEGKRSYTWNVKVR"
     gene            complement(6590..7633)
                     /locus_tag="VFF28_06215"
     CDS             complement(6590..7633)
                     /locus_tag="VFF28_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45249.1"
                     /translation="MKKLVTFCLIGIFLISGCARLKELYGIQPQGKEEIISIDEIKVQ
                     AKENDSKDDTLKPLPDDIPRIEDIPKPQKLNVSNIKKADSKVILVKETELVSLKPRAT
                     DPDQDKLVFGFTAPLDQEGRWQTKYGDAGEYAVTISASDGTLTAIKEVLVIVSKKEEA
                     PTIDESLPKESAVEAKENSKVSFSIKSSDLNKDSLTYSWKLDGKAVSAKDFYSYDISY
                     EDAGQHTVKVAVTDGTKEASKVWSVKVENINRKPELAKILDIKVKENEKILLSPKAAD
                     PDKEDKVTYTIANDKFTKVGDTFEWKTSYDDAGDYAMVLSATDGKDSSTQTVKIKVEN
                     VNRPPVIEDITLG"
     gene            complement(7960..9141)
                     /locus_tag="VFF28_06220"
     CDS             complement(7960..9141)
                     /locus_tag="VFF28_06220"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX45250.1"
                     /translation="MALNNIRLSYLARFLRDLQFFGAIIVPFFLDWLQVSYTQFFLLE
                     SWFVLWIVLLEIPTGIVADKYGRKISLALGVLFFGIDLLLLGLLRDYYLLYIAEFIGA
                     LGYTLISGADQALIYDYLIEQKKQKDAKHYLARYEAAGTLGLIISLPLGSIIADSASY
                     PFSLALTFILSGACTILSVFFFLMMKEPKRRRPTENFVRMGIDGLKYLNNHKVLRAFA
                     INSVFISSVTSFIFWFYQPMAGSAGIGLRYYGFIGAGFNLFAVLLLMNTKKLEKVFGM
                     KNLLMYSAIVPAVLFIGAGLVNGLFLTLLAIFLIAGLKMLRTPILSDYMNRYIQGKNR
                     ATILSSISLVERATTAILYPVVGFFADISLSYVLIFLGILTLVFAIITRLEESHLVCH
                     R"
BASE COUNT         2350 a         2025 c         1865 g         2981 t
ORIGIN      
        1 tggtaaagtg tcattgggcc tcacctcttt tttgaggctc atcattttac caacagataa
       61 tcacagaatt agccatccta taagtgttac gcattcatcc ccaaccacaa gtggtgggga
      121 tttctgctaa gaaatttaat cgtgtcccat ccatctttgt atgtctttgg tcttctatct
      181 ctatttcttt ctctctttcg ttctaactta catccaaggc aaaatctttc atttggatga
      241 aagttactta agggtttgaa aagatcgcca catctttcac atttagccat aggcctatct
      301 tctggcttgg gatatctcct cagtttagaa agaatttttt ctctattttt tttatagaaa
      361 gctcttactt tttgtttttc tagttcagga tttttccaat aaatttcctt tttgaccatg
      421 ggaaaattga taaaatgctc acttactaag tttaaacgcc cctgccggga atcgaacccg
      481 ggcctcaaga tccgcaatct tgcattctga tccactatac tacaggggcc tagggcaata
      541 agattatgat tctgtttatt aatcttgcca gaactaaaaa agtgcgtaga tgaacggaga
      601 aagcgcgctg ctctgcccaa atatgatgag cgctccgacc aagacaagca tgattattat
      661 cggggcaagc caccaggctt tcctgacctt aaggaaatcc cagacctctg cgagcatcga
      721 cttattatta gacatggtat tttgctaatg gcggatattt atatatttaa tcatttgcga
      781 gcttttctga gtcccatgcg tccttaatgt tatcatccct ttttactagg aaattaccta
      841 tgaccagaca gtctatgcct gtccccatca tgcacttata cgcatcataa gggctgcaga
      901 cgataggctc tcccctgatg ttaaaggatg tgtttatcag gataggaatg cttgagagcc
      961 tgccaaactc ttttatgaca tcatagtaca atgaattgtc acttctcttg acagtctgca
     1021 gccttccgct gccatcgaca tgggttacgc ttggaatctt tgaatgccat ttctccttaa
     1081 tcggatatac catgagcata taatctgtgg gctctggaat cgggatgtcg gtgtcaaagt
     1141 aatgctttgc atcatcgtcg catacaacag gagcaaaagg cctgaatttt tccctatgct
     1201 ttaccttggt gttcaacaat tcctttgcat gaggatttgc agggtttgca aggatgcttc
     1261 ttgcgccaag agctcttggg ccccattcca tgccaagctg gaaccagccg acaacattat
     1321 tggtatgcag cagagctgcg gtctttttta tcagctcata ctcgtccttg aattgagtgt
     1381 aggttatctt gtttttatca agaaagccct ttatgtctgc atccgtatag cttggaccga
     1441 gataggcagt tgccagcttt tttctttcct ttccaaggat agagtggtag atgtatgatg
     1501 caacaccgac gcttgtccct ccatcgcctg gatcaggctg tgcccagaag ttcttgaact
     1561 ttgtatttct tagtatcttg ccgtttgcaa cactgtttag gccgcatcca ccggcaatca
     1621 ccagactgtc acagcatgtc tctttatgca catggtttag tatcttgaaa agcgcctctt
     1681 catagatgag ctgcaaagct gctgcgatat ccttgtgcct ttttgttatc tcttcttctg
     1741 gccttcttat cggcccgtct aagagggtgc atagtttcct acttggcatc ctacctgcaa
     1801 aatgatattt gaaataagac atatctagcc tgaaacttcc gtcctctttt agatcgataa
     1861 cttgaataag ctttaggtaa tatggatttt tttctttgtc catctcgcca taggggcaga
     1921 ggcccatgac cttatactct gagttgttga ctgtaaatcc aagataggct gttatcgtcg
     1981 aatagaatag ccctaaagaa cttggaaacc ttatctcttt ttttaaggtt atatcgctgt
     2041 cttcgcctat accataagag gtggtggtcc actcgccgac gccatcgatg gtcactatcg
     2101 ctgacttttt gaaagggctt aacagaaaag aacctgctgc gtgggcaaga tggtgctcga
     2161 tgaaaagcac atctttcttg tatttgagct tttttatcgc tttcatcacc ctgagctttt
     2221 cgtttatcca tgagggagta gaacttaaga acgtctttag acttcttgga aacatctcaa
     2281 ggtgctggct tagcacccgt tcaaacttta tgaggggctt ttcatagaag ccgatataat
     2341 caatttcgtt aatcgttatt ttttgggaag atatgcagta atttatcgcc tcttttggaa
     2401 aagaggtatc gtgctttttt cttgtaaatc tctcttcctg ggctgcgcac acgacgactc
     2461 catcctttag caatgctgcg gatgaatcat ggtaatagca acttatccca agtatgtaca
     2521 tattaacctc aaaattgcct gtaatgtgac tctatcggct tttttcccaa gtttaggtct
     2581 gaccagtagc tttctttttt cttctccata tccataaagt gctttccaaa gagctttgcg
     2641 ataatggcag tgaggccgac tcctaaaaag tacactatag tcaataaaac tgagttgatg
     2701 ataacggata gattggtgcc aaaagagtgc atgcctttct tgaagccttt gggaaaactc
     2761 attttaacag cctctttgtc atgatacctg caagaagaac aggcgctgat atgctgaagg
     2821 ctttaagcaa ggcaaagttt tctgtcttat ttgcataaac aaggatgaga aaaatgatgc
     2881 aagtaaaaaa tagatatgag aaaataaaac cgctgcctat accgatcttc tctgtaagac
     2941 ccccatcgtc aaactgcatc aaataaaagt taaatacgtg tgtttataaa tccttctctt
     3001 aataatcgct caaacttatc tggccttttc ttttgttttc ctttaccggc tcgacctttt
     3061 ctcctacctg ttcttttatg cctatagcga tgttctttcc caagatttga gagagctttg
     3121 tgatatctgc tgacttgaca tcaccaagat ctttaatcct attgttgaaa agctttcttg
     3181 ccctgaccct gccgatgttt cttagattta gaagagggat gagctcttct cttactccgc
     3241 tcttgagcct aacccttagt tttgcaatgt cttttaggag cggctggaac tgcatgagcc
     3301 ttgccatctc ttcacttgta taaagaagcc aatcggctat gtcaagcttc acttttatct
     3361 cgcctggcct aatatcataa gtttccaaca agtactcttc gtctttttca tcgagccatt
     3421 cttggaaaaa catggccagc ttaatggagt ttagaaagtc gtcgtaatcc tggtcgtata
     3481 tgtttggctc ttcttctaac aagaaggtga aattttttgt gagcacttcc tggaccttat
     3541 cgtattcttt tgcctttact tttaataacg gccttagctc taaggtatga gagatcatct
     3601 ggacaaagga gaaaacattt gtctcttttg atgctgcttt ttttatgcaa tcgatgaatt
     3661 tttttgcggt aagcggatcg atgtaaagct ctgctaccct ttttccaata atcgtcgggc
     3721 ggtatctttc atcacctaat tcatctgcac cctggaattc atccttgttt cccataataa
     3781 actcccattc ctgcagtagg ccaatcatct tttgcagcat ctggttgagt tcatacatgt
     3841 cgccgaactg gtgagcccag aatgtctttg agaaaaaatc aaagagctgc tgctttgtat
     3901 tgacaaagcc tgatgcgatg aggcttagaa tataagtgcg caagacaggc tcaactgcaa
     3961 gctttgaata gatgttttct ggtttaccgt ttatgtatct ttcgaggagc tcttcttttt
     4021 cattctctga ctgggcaatt atgatcgctt cgccgaacct gtcaaatcca ggccttcctg
     4081 ccctgcccca catctggtga atctcaagaa caggcagata atcaagacca cggtggccat
     4141 atctttttgg gtctttgact atagagcgga atgctggaag gtttaagccg taggcgagag
     4201 tgggggtggc gcagattatc tttattgcgc ctttcttgaa gttttcttcg ataagctccc
     4261 tttgctttgc aacaaggcct gcgtgatgga aggcgatgcc ttttcttagg caaatagaaa
     4321 gcctttcgca ttgctttgta ggctttgtga gagcatgaag agatttatct gagatttcct
     4381 gcaagtctat accccttatc tttttactga tatcctctgc tgtcttttct gctgagcgct
     4441 ttgtgtttac gaagataatg gcttgctttc caagatttat agtgtccaga gctaggtcta
     4501 ttgcgggatt ttgctccata cttatggtta agaaaagaga tatttaaggt tattgcaaat
     4561 ctctgacatt atctaacctt tacattccat gtgtaggatc tcttgccctc gccgtctgag
     4621 acaaagacag ttatcttctt tgtgccagcg gttttaaacg tccttgatat gcttgggcca
     4681 agctctcttt tgtcaaacag gccaaactgc caggtgtatg caaggtcgtc accatctggg
     4741 tcttctgcta ttatcttgaa tatcgccttc tcgcctttct ttgcggctgt aaccttagat
     4801 gctcctgtaa ttttcggcgg cctgttcttg ttatggacag tgatatctat cggctgggtg
     4861 atattgttgg taccatctgt agcttttaag tttattgtca acaatgtcga gccttctttt
     4921 acggtgtcgt aatccggcgt gtatatcagc ttgccttctt ttatggcagc gttttttggg
     4981 agagagtctt ttatgaattc aatcggatct tcctcaggat caaaaatgag caagggaatc
     5041 ttaatctcct gggcttcact tatctccaca gcctgcatgg ggctgaactg aggcggcctg
     5101 ttgatatcgt ttacagtaat gtccaccgtc ttctcatcgg tgaggaatcc gtctgaagcg
     5161 atgatactta ccttatagtt tcctgcatcg tcaaaggttg tcttgtatct gtttacatcg
     5221 aaccaacctc tgtatgaata ggagagtttg tcaccgtcag gatctgcggc ctttggctcg
     5281 atgtttatct cttcaccttc gtctacgatg atcggctcta ttgtttccaa caccggaggc
     5341 tgattaatgt tttttatgaa tatagtagca gactgtttct cttcactatc tgctgaagct
     5401 gtgaatgtta tcttgtatgg agtgtataat aagtgaaggt tatccagtgc ttttttaaca
     5461 aggctatctt tgcttacagt agctgggctt gtctgccatt cgaaaacatt gtcttttagc
     5521 gctgctcctt ctggcagatt ttgagctgaa tattttatct tttctccatc aggatcatct
     5581 gcctcgaggg ctattgtaat cttgtctccc tctgtaatcc aggagtttgc aataggcttg
     5641 aacgagagcg ggcggtcgat gttttttaca accaccttta tcttttttgt tgagatatat
     5701 ttgccgtcct tgatggttag cgcaatatta tagatacctg cttcatcata gccaatctgc
     5761 cagtaattgt cctggcctag agggtctgat agctcatagt ccagattata ttttttaaaa
     5821 tcaggcaaat ctaactgaag aacttttcct tcttctgcct caatatcttt aaacgatgag
     5881 aagatggctg attccctgtc gaccttttta acgcttacct gccaagcctt gtctatcgtg
     5941 acatcgccgt cggagatgac cgcttttatc ttgtgagtgc ctgcatcacc gtaataggga
     6001 gaatactcaa aggactgacc ccaggcagct tctttatcgt caattttcca tgaataggtc
     6061 aaagaatcct tgtttatgtc tgaggctttt atgccaaagg ttaatttccc agtctcttct
     6121 acctccaggt ttatggtccc tggctttgca gaatcgatga ccggagggat atttttcttt
     6181 tttactacaa gaaggacgtc ttttgtcgtc actgcctctc catctgaggc gttgatggtg
     6241 ataatgtatt cgccttggtc gccatatcct gtctgccact ttccatcgct gtccaagggc
     6301 gggctatagc tgtatgttat gctgtcttta tctgcatctg tcgcctgggg ccttaatgag
     6361 acaggctctg tctcatagac cgtcaaggtc tttaccgcgg ctgctatgct tatgcaaagc
     6421 accaatacaa ccataattaa taaaaattgc ctgttcattt tcccccactg ccgtctagga
     6481 cggaaaaata aaaaatccca aaccagagcc ccagctctgg ggtatttttt aagcctcaag
     6541 cttaagactg ccgcagcaaa ctgcggagta tgaactttag tttgagcact caacctaagg
     6601 tgatgtcttc gataacagga ggcctattta cgttctcaac cttgatcttc actgtctgag
     6661 tcgatgagtc cttgccatct gtggcagaga ggaccatagc atagtcacct gcatcatcat
     6721 aggatgtctt ccactcaaag gtgtcgccaa cttttgtaaa cttgtcgttt gctattgtgt
     6781 acgtgacctt gtcttccttg tcaggatctg ctgcttttgg agacaataaa atcttttcgt
     6841 tctcttttac cttgatatcc agtatctttg caagctctgg ctttctgttg atattttcaa
     6901 ctttcacaga ccaaaccttg cttgcctctt ttgtgccgtc tgtgactgca accttgacag
     6961 tatgctggcc tgcatcctca tagcttatgt cataggaata gaagtctttt gctgacactg
     7021 ccttgccgtc aagcttccat gaataggtaa gtgagtcctt gtttaggtct gatgatttta
     7081 tactgaatga aactttcgag ttctcttttg cttcaacggc actttctttt ggcagggatt
     7141 catcgatggt cggagcctct tctttcttgc ttactattac caggacctcc tttatcgctg
     7201 tcagggtccc atctgatgcg gagatggtca ctgcgtattc tcctgcatcg ccgtattttg
     7261 tctgccatct tccttcctgg tcaagcggag cggtaaagcc gaacaccagc ttgtcctggt
     7321 ccgggtcggt cgctcttggt ttaagtgaga caagttctgt ctcttttaca agaataactt
     7381 tagagtctgc tttctttatg tttgaaacat ttaatttttg gggctttggg atgtcctcta
     7441 tacgagggat atcgtcaggc aaaggcttta gcgtatcatc ctttgaatca ttttccttcg
     7501 cctgcacctt tatctcgtca atagagatta tctcctcttt tccctgaggc tgtatgccat
     7561 aaagctcttt tagccttgca catccactaa tcagaaatat accaataaga cagaaagtca
     7621 ccaatttttt catgaaaatc ccctccactt ataatgttgt tattttttaa tacacaatag
     7681 gtatataaac tttgtggtag ttactaaaag cgaagcttaa gctattcaag tgttgtttta
     7741 gattatgatg gatttaggag tgctgaacca tttaaataaa tgatgttgaa ccagttatat
     7801 acgacttgga gggtaagcgg cgatatgctc agtaattcac atcacagatg gaattgatcc
     7861 tgttgaatgg tgaattccat ttaccctgga cacgattata aatcctgaaa aattattttt
     7921 gactcagtgc cacaagcatg gctatggcaa ccggtttgct tatcgatggc acaccaaatg
     7981 gctttcttcc agccgcgtga taatcgcaaa gaccaatgtt agtataccca gaaatataag
     8041 aacatagctt aggctgatat cggcaaaaaa tcccaccact ggatataaaa tagcagttgt
     8101 cgccctctcg accaatgata tcgatgagag aattgtcgca cgattcttac cttggatata
     8161 cctgttcatg taatctgaca ggataggagt ccttagcatc ttcaatcctg caataaggaa
     8221 gatcgctaga agggtgagaa acaggccatt taccaaccct gctccgataa ataaaaccgc
     8281 agggactata gcagaataca tcagcagatt tttcataccg aagacttttt caagcttttt
     8341 tgtgttcatc aacaacaaga ccgcaaacag attaaagcca gcgcctatga acccataata
     8401 cctcaagcct atacctgcac taccggccat tggctgataa aaccaaaaga taaaggaagt
     8461 gacggatgaa ataaaaactg aattgatcgc aaatgccctt agaactttat ggttgttaag
     8521 atattttaga ccatcaatgc ccatccttac aaagttctct gtcggcctgc gcctttttgg
     8581 ctctttcatc ataaggaaga agaaaactga gaggatggtg caagcaccgg agaggataaa
     8641 tgtcaaagcg agtgagaatg gatatgaagc tgaatcagca attatcgagc caagaggcaa
     8701 tgagataata aggcccagtg ttccggctgc ttcatatctt gcaaggtaat gctttgcgtc
     8761 tttttgtttt ttttgctcga ttaaataatc gtagatcaat gcctggtcag cacctgagat
     8821 cagggtataa ccaagagctc cgataaattc agcgatgtaa agaagataat aatcccttaa
     8881 aagcccaagc agtagaaggt caatgccaaa gaataaaaca cctaaagcca aagagatttt
     8941 tcttccatac ttatcggcga ctatacctgt gggaatctcc aagaggacta tccataagac
     9001 aaaccatgat tccagaagga agaactgggt atagcttacc tgcagccaat ccagaaagaa
     9061 agggacgatt atggctccaa aaaattgcaa gtcccttaga aatcttgcca agtaacttaa
     9121 acggatgttg ttaagagcca taagcaactg attatctctt ctaaggtata taaacattag
     9181 tgtgactaga cttttgcact tatcgacgac aagcaaaaac a
//
LOCUS       DASRUK010000140         3021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_113859, whole genome shotgun sequence.
ACCESSION   DASRUK010000140 DASRUK010000000
VERSION     DASRUK010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113859"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..1688
                     /gene="proS"
                     /locus_tag="VFF28_06225"
     CDS             243..1688
                     /gene="proS"
                     /locus_tag="VFF28_06225"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HZX45251.1"
                     /translation="MPKEKESIGITVKKSEDISNWYTEVIQKADLADYTGVSGCIVFK
                     PYSYAIWEKIVRAVDDRLKAMGVKNAYFPLLIPEKLLQKEQAHVKGFTPEVAWVTHAG
                     DTKLEERLAVRPTSETIMYDSYKDWIRSWRDLPLKINQWNNVVRWEFKHPVPFLRTRE
                     FLWNEGHTCFATKEEAEKECIDILNMYGEVLEDYLSLYGIKGKKTEHEKFAGAEYTLS
                     IELLLPSGRAIQGPDSHHDGQNFAKAFDIKFLDQNEKEQYVWQNTWAITTRMIGVMIM
                     VHGDDKGLVLPPKLAPIQIAIVPIIFKDSKEKVIKKAKEIKEKLKGYEVELDDREEYS
                     AGWKFNQYELKGVPIRIEIGPKDLEKEHVVIVRRDTLKKEFVKFADISSKVESIMESI
                     QENLYNNSKKLLEENTVEVKSMDEFVRAIKNKKMVKALFCGEPSCEDEIKEKTGGATS
                     RNIPFDQKHIKGKCVNCGKEAKYSVYFAKGY"
     gene            complement(1685..2551)
                     /gene="prs"
                     /locus_tag="VFF28_06230"
     CDS             complement(1685..2551)
                     /gene="prs"
                     /locus_tag="VFF28_06230"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HZX45252.1"
                     /translation="MLVIGCSHGKHIAPKIAKLNAYPYSELEVTHFPDSEIKLRFKID
                     VKGKEIGLVQSFYHNVNDCIIETLFAAETAFDLGAKKISLIAPYFPYLRQDRRFNPGE
                     CISLRTIAKNIDDDFDEVYIMDPHLHREKTLKHIFKVKSHKITANPLIVDYIGTIKNA
                     LIVGPDEESYKWAEKVADTIGCESAIMTKNRRSAYDVEVSLNKKLDLKKKNIIIVDDM
                     ISTGNTIIETSKLLRKLGAKKITSICVHGIFANHALERLKANKINVVACNTIPNKAAK
                     IDVSGIIAESIR"
BASE COUNT          941 a          587 c          643 g          850 t
ORIGIN      
        1 cttctcaaca acttcgtaac cttccatctt catttgaatt ggttttctca tgtagttaca
       61 atgtaactac aagaactaaa taaatatttc ggtgctcaag tatataatta gaattagtgg
      121 gataaagcta aacgattacg gtttatccct tatagctttg atcattatcc ctatctttgt
      181 tgccaggagt agttttcttg ccatagaaac atttattaaa tcctttacaa agtcttcctt
      241 taatgcccaa ggaaaaagaa agcatcggaa taacagtaaa gaaatcagaa gatatctcta
      301 actggtatac tgaagtcatt cagaaagcag accttgccga ttatactggc gtctcaggct
      361 gcatagtctt taaaccttat tcctatgcta tctgggaaaa aattgtaaga gcagtcgatg
      421 acaggctaaa agcaatgggt gtaaaaaatg cttattttcc cctgctaatc ccagaaaagc
      481 tgctccaaaa agagcaagcg cacgttaaag gatttacacc tgaagtagca tgggtcactc
      541 atgcaggaga cacaaagctt gaagagcgtc ttgccgtaag gccgacttct gagactataa
      601 tgtatgacag ctataaagat tggataagaa gctggagaga tttgccctta aagataaacc
      661 aatggaataa cgtagtaaga tgggagttca agcatcctgt cccattcctc aggacaagag
      721 aatttctatg gaacgaaggc catacttgct ttgcaacaaa agaagaggca gagaaggaat
      781 gcattgatat ccttaacatg tacggagaag ttcttgaaga ctatctatca ttgtacggca
      841 taaaaggaaa gaagacagag catgagaaat ttgcaggagc tgaatatact ttatcaatag
      901 agcttctatt gccaagcggc agagcaatac aaggtcctga cagccatcac gatggccaaa
      961 attttgcaaa agcttttgat ataaaattcc tagaccaaaa cgagaaggag cagtatgtct
     1021 ggcagaatac ctgggcgata accacaagga tgataggtgt catgataatg gtccatggcg
     1081 acgataaagg ccttgtcctg ccgccaaagc ttgctcctat ccagattgcg atagttccga
     1141 taatatttaa ggattcaaaa gaaaaagtca tcaaaaaagc aaaggagata aaagaaaagc
     1201 tcaaaggata tgaggtcgag ctggatgacc gcgaggagta ttccgcaggc tggaaattca
     1261 atcagtatga gctaaaagga gtgccgataa gaatagagat aggtccaaaa gatcttgaga
     1321 aagagcatgt tgtgatcgtc aggcgagata cactgaaaaa ggagttcgtc aaatttgcag
     1381 atatatcttc aaaagtagag tcaataatgg aatctataca ggagaatctg tataataact
     1441 ccaaaaagtt acttgaggag aatactgttg aagtaaagag tatggatgag tttgtcaggg
     1501 ctatcaaaaa caagaagatg gtaaaagcat tgttctgcgg tgagccttcc tgcgaagatg
     1561 agataaaaga aaagacagga ggcgcaacct caagaaacat tccttttgac caaaaacaca
     1621 tcaagggaaa atgtgtgaat tgcggtaaag aagccaagta ttctgtgtat tttgcaaaag
     1681 ggtattaacg gatagattca gcaataattc cagaaacatc aatctttgca gccttgtttg
     1741 ggatagtgtt gcaagccacg acatttattt tgtttgcctt gagcctttca agcgcgtggt
     1801 tggcaaatat cccatgcaca catatagagg taattttctt ggcacccagt tttcttagta
     1861 atttgctggt ctctattatt gtatttccgg tcgatatcat gtcatcaact atgattatgt
     1921 ttttcttctt taggtccagt ttcttattca agctaacctc aacatcataa gcacttcttc
     1981 tattctttgt cattatcgct gattcgcatc ctatcgtatc agcgaccttt tcagcccatt
     2041 tgtagctttc ttcatcaggt ccaacgatca aagcattctt gatagtccct atataatcta
     2101 cgatgagggg gtttgcagta atcttgtggc ttttcacttt gaatatgtgt ttcagcgtct
     2161 tttccctatg aaggtgtgga tccatgatgt agacttcatc aaaatcatca tcgatattct
     2221 tagcgattgt tctaaggctt atgcattcac caggattaaa ccttctatcc tgcctgaggt
     2281 aggggaaata tggagcgatg aggcttatct tctttgctcc aaggtcaaag gcagtctcag
     2341 ctgcaaacaa agtctcgatg atacagtcat taacattgtg ataaaaggac tgtaccagcc
     2401 ctatctcttt tcccttgaca tcgattttga atctcagctt tatctcagaa tcaggaaaat
     2461 gggttacctc caattcagaa tagggatatg cgttcagctt tgctatcttt ggcgctatat
     2521 gttttccatg cgagcatccg atgactaaca taacaagtcc ctagtcttag ggatatttaa
     2581 gcgttttgct tgctcaagac ttgggttaat accccgtagc ttgttgccga acgaagtgag
     2641 cgcaactaag aaacgaagtt tctgttgtta cggcttcacg agcacagttt gaggcttaaa
     2701 gaaaataccc tcagctcggc tagcggttgg gactttttat tgaacccaaa ccctgcactg
     2761 ccatgggatt tttaccacgt gtcaagaacc gccgacctaa aggtcggggc gtgttcttga
     2821 gcatgctcag aaatcgctgg cttactctgg gctaaagtat cagagcgtgc gcccgaagcg
     2881 atttctgaca tgtcaaaaac caccagtcac cgctcctttc agtcgctata gactggtggc
     2941 atgacggtta agtggttttt gacgcttgaa acactaaaaa atccatagga tttttggtgc
     3001 accaaaaatt tgcaatggca a
//
LOCUS       DASRUK010000141         6644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2441746, whole genome shotgun sequence.
ACCESSION   DASRUK010000141 DASRUK010000000
VERSION     DASRUK010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2441746"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..1894)
                     /locus_tag="VFF28_06235"
     CDS             complement(14..1894)
                     /locus_tag="VFF28_06235"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004557385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HZX45253.1"
                     /translation="MDGISKIRKRDGRIVELAPEKITNAIHKAAQSVAVKEGRNAEKK
                     ECEELTNKVMTILEYQFTEKEVPTVEEVQDIVERVLIKSGHAKTAKAYILYRQQHAKI
                     RETKDMLVDVSKTIKAYVDQEDWKVRENSNEDYSFSGLLLYAAGKVIGNYALNEIYTP
                     EIKEAHVKGYFHLHDLSNAVIGYCAGWSLKKLLKMGFGGVPNKVDCKPAKHLNTVIHQ
                     MVNYIGCLQMEFAGAQAFSSVDTLLAPFVKIDNLSFKETKQCIQQLVFSLNIPSRWGS
                     QYPFSNLTFDWRVPEDMKKEKAIVCGVEQDFTYDECQKEMDIINKAFLEVMLDGDARG
                     RIFTFPIPTYNLNKDFDWNSPNANLLFEATAKYGMPYFQNYIGSNLDPRSIRAMCCRL
                     NLDQNQLVKRPGGMWGPGDATGSIGVVTLNMNRLGYEAKTKEEFYQKLSYYMGLAKTS
                     LEIKRVMIERNLKNGLMPFTKIYLGTFKNHFSTIGLCGMNECCLNFLGKDLSSDESVD
                     FTIEVINYMRGKLVEFQQETGSLYNLEATPAESTAYRFALLDKKLYPDIITSGTDEPY
                     LTNSSQLPVDKTDDVVMALEHQNKIQPLYTGGTIFHAFLGEKVSSGEACKQLVKKIAE
                     NT"
     gene            complement(1916..2368)
                     /gene="nrdR"
                     /locus_tag="VFF28_06240"
     CDS             complement(1916..2368)
                     /gene="nrdR"
                     /locus_tag="VFF28_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HZX45254.1"
                     /translation="MRCPYCNSEETKVIETREGEEPDVTRRRRECLSCSKRFTTYERV
                     EIIELRVMKKNGDIESYNRNKIVNGILKACEKRPVKIEKVERIVDEIETELRKKESIE
                     IPSKEIGEIVMDKLKGIDQVAYIRFASVYREFKDIKSFEKELEKIKSD"
     gene            complement(2581..3279)
                     /locus_tag="VFF28_06245"
     CDS             complement(2581..3279)
                     /locus_tag="VFF28_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45255.1"
                     /translation="MKLKEKQKNPYVKTREYVPAIATYIAREEGYAMLVEARKAGGRY
                     VVLVTVEDNEKRTISKAARLYNTNPLEEVGEYVIDNMLKEIKRIVTGRDYQSKQVLIE
                     TIVKPNAANASGLEALTDEHGKQTPGENDDRYQVKRTNKMYERQGPEPPIGPPSILAR
                     DSIPFKTFYNFLGAVGHEDQSGESKSARHDKETRVIRFNPPIKAGALAFGVQGYQKAA
                     YGSTAKAYVAQKAA"
     gene            3335..3643
                     /locus_tag="VFF28_06250"
     CDS             3335..3643
                     /locus_tag="VFF28_06250"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HZX45256.1"
                     /translation="MPFHCKRCGQCCNPPHIFKQDIKRLESSGLKDFVYTDNLDHTYL
                     KDKGGWCMFLKKGKIAECTIYMIRPKTCRLYPSELNNGDCRPIELAFDDYLENIPSHD
                     "
     gene            3697..4632
                     /locus_tag="VFF28_06255"
     CDS             3697..4632
                     /locus_tag="VFF28_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HZX45257.1"
                     /translation="MPKTDVGGQAVIEGVMMRSKNDIVTAVRKKGKIIYKEEKLKQKP
                     KWMGFFFIRGIVNLIDMLVIGIKTLNWSASQAGEEDEELSSTAIWITLLIAFAFSIGL
                     FMALPYALTYLLGVRETESPVWFNIIDGIIKAAILVAYVYLISLMKDIRAVFQYHGAE
                     HKAVFCYEAGKELTIKNAKVYSTKHPRCGTAFLMIVIIISIFLFSFIPILINTAYPLF
                     ESLPWLIRRITLFGTRLLLLPVVAGISYEALKFGAKHQGNPLFRALTLPGLWLQNITT
                     KEPTEKQLEVGIAALKKVTENGNMSKTPLTKAERG"
     gene            4716..5276
                     /locus_tag="VFF28_06260"
     CDS             4716..5276
                     /locus_tag="VFF28_06260"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK2/MTQ2 family protein methyltransferase"
                     /protein_id="HZX45258.1"
                     /translation="MFLTYEPAEDSFLIQRYVKKYAKGIVLDMGTGSGILAEEAAASK
                     KVVKVFGVDKNEKAIIHCIRSQKSRKITYAMSDLFSLFKNYKPYMGIKFDTILFNAPY
                     LPSDPNVKDMALDGGKKGHELIVKFLNKAKAFLKPMGKILLIFSSFTGKETLDNYIST
                     HHWKYKEIDKVHISFEDIYLYLIENE"
     gene            5269..5868
                     /locus_tag="VFF28_06265"
     CDS             5269..5868
                     /locus_tag="VFF28_06265"
                     /inference="COORDINATES: protein motif:HMM:NF013772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45259.1"
                     /translation="MSELTDLTYLAKGCRGIVYTADYNGKKVVVKKEKEGSKAINRLQ
                     NEANFLKILNKESIGPKFYFCDETSLVSEYIEGELFPEWLKKANSKDSLKVIRDIYAQ
                     CFKMDKLKISKEEMHHPPKHILIRKGKPVMIDFERCHKSPEPTNVTQFSSYMLSRNIT
                     TSLNKKNITVDKEGLISSCKEYKANMTREKFKKVLSAIK"
     gene            5865..6176
                     /locus_tag="VFF28_06270"
     CDS             5865..6176
                     /locus_tag="VFF28_06270"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019556673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HZX45260.1"
                     /translation="MNLSFNEKVWALTAKIPKGKVTTYKIIAEKLGTKAYRAVGAALA
                     KNPYAPKVPCHRVVCSDGRVGGYNGSMDNKEKTRLLKKEGVKINNGKISHFKAGEIIS
                     Q"
BASE COUNT         1925 a         1450 c         1240 g         2029 t
ORIGIN      
        1 aaaaataagg cagctaggtg ttttctgcta tcttcttgac gagctgcttg catgcttctc
       61 ctgacgatac cttttcgcca aggaaggcat gaaatattgt acctcctgtg tataatggct
      121 gtatcttgtt ctggtgctca agagccatga caacatcatc tgtcttatcg acaggcagct
      181 gtgatgagtt tgtcagatag ggctcatctg tgccggatgt gattatatct ggatagagct
      241 ttttgtcaag aagcgcaaac ctataagctg tcgactcagc aggtgtggct tcaaggttat
      301 acaagcttcc tgtctcctgc tggaactcta caagttttcc tctcatgtaa tttatcactt
      361 ctatggtaaa atcaacactt tcatctgagc ttaagtcctt gccgaggaaa tttaaacagc
      421 actcattcat cccgcaaagc cctatcgttg aaaagtggtt cttgaaagtt ccaaggtaga
      481 tctttgtaaa cggcattaaa ccattcttaa ggttcctttc tatcatgacc ctctttatct
      541 caagcgaggt cttggcaagc cccatataat atgaaagctt ctggtaaaat tcttccttgg
      601 tttttgcctc ataccccagc ctgttcatat tcaatgtcac aacgcctatt gaccccgtcg
      661 catctccagg cccccacatg cctccaggcc tttttacaag ctggttctgg tcaaggttaa
      721 gcctgcagca catcgccctt atgctccttg gatcaaggtt tgagccgata taattctgga
      781 aataaggcat cccgtacttt gcagttgcct cgaatagaag gtttgcgttc ggagagttcc
      841 agtcaaaatc cttgtttaga ttatatgtgg ggatagggaa agtaaatatc cttcctcttg
      901 catctccatc aagcatcacc tcaaggaaag ccttgttgat tatgtccatc tccttctggc
      961 attcgtcata tgtaaaatcc tgctctactc cgcagacgat agccttctct ttcttcatat
     1021 cctcaggcac tctccagtca aatgttagat tgctgaaagg atactgtgat ccccaccttg
     1081 aagggatgtt tagtgagaat acaagctgct gtatgcactg cttagtctcc ttaaacgaca
     1141 ggttgtctat tttaacaaac ggtgcaagca atgtatctac agaagaaaat gcctgggctc
     1201 ctgcaaactc catctgcagg cagcctatat agtttaccat ctggtggatg actgtgttaa
     1261 gatgctttgc tggcttgcaa tcgaccttgt ttggcacacc gccaaatccc atcttcaata
     1321 attttttcaa agaccatcct gcgcaataac ctatgactgc attggacagg tcatgaaggt
     1381 gaaaatatcc ttttacatgc gcttccttta tttcaggagt gtatatctca tttagtgcat
     1441 agttgcctat cacctttcct gcggcataca gtagaagccc tgaaaagcta tagtcctcat
     1501 tgctgttctc cctgactttc caatcctcct ggtcgacgta tgcctttatt gtcttgctga
     1561 catcgacaag catgtctttt gtctccctta tctttgcatg ctgttgcctg tatagtatgt
     1621 atgcctttgc agtctttgca tggccggact tgataaggac tctttcgacg atatcctgca
     1681 cttcttcgac agtaggcact tctttttcag tgaattggta ttcaagtatc gtcattacct
     1741 tgtttgtgag ctcttcgcat tccttttttt cagcgtttct tccttccttg acagcgacac
     1801 tttgtgctgc cttatggatt gcatttgtta ttttctcagg agctaactca actatccttc
     1861 cgtctctttt tcttatcttt gaaattccgt ccatcctaac cacctcattt aagcatcaat
     1921 cactctttat cttctctagc tctttttcaa aactttttat atctttaaat tccctataaa
     1981 cagaggcaaa cctgatatat gcaacttggt cgatgccttt tagcttgtcc atcactatct
     2041 cccctatctc tttgcttggt atctctatgc tctctttttt tctgagctct gtctctatct
     2101 catcgactat tctttcgacc ttttctatct tgacaggcct tttttcacag gctttgagta
     2161 ttccatttac gatcttattt cgattataag actctatatc accgtttttt ttcataaccc
     2221 tcagttctat tatctctacc ctttcatagg ttgtaaatcg ttttgagcat gaaaggcact
     2281 ctcttcttct cctggtgaca tcaggctctt cgccttctct tgtctctatc accttcgtct
     2341 cttcactgtt gcagtatggg catctcattt tttcctgtcc tagtgttttg tgcttatcta
     2401 gagcactaac tatagtagta tgacctgttt ataaaccttg ggttcgacca acactatata
     2461 ttgtgttttg tcctgtaaaa atctctatac ctagtcaact ttccagaact tcttatattt
     2521 aaatatttgt gtcccaaaaa atatattatt taaagtaaat aacccaaata gtgaaaagaa
     2581 ttaagctgct ttctgggcaa cataagcttt ggcggtagaa ccataagcag ctttttgata
     2641 accctgcaca ccaaatgcaa gtgcacctgc ctttattggt ggattgaacc ttataactct
     2701 tgtctctttg tcatgacgtg cacttttgga ctcacctgat tgatcctcat gcccgacagc
     2761 accaagaaaa ttgtagaatg ttttaaaagg gatgctgtct cttgcaagga tgcttggagg
     2821 gcctattggt ggttctggcc cctgcctttc atacatttta ttggttcttt ttacctgata
     2881 tctatcgtcg ttctctcctg gggtttgttt tccatgttca tcggtcagcg cctctaagcc
     2941 agaagcattt gcagcatttg gttttactat tgtttcaatc aacacctgct tgctttgata
     3001 atcgcggcca gtaacaatcc tttttatctc ctttagcata ttatcgatga catactcacc
     3061 tacttcctct aacggattgg tgttgtatag tcttgctgct tttgatattg ttctcttttc
     3121 gttatcttct acagttacaa gaacaacata tcttcctcca gcctttctag cttctacaag
     3181 catagcatat ccttcttctc tggcgatata tgtagcgata gcaggtacat actctcttgt
     3241 ttttacataa ggatttttct gtttctcctt caacttcata gtattttagg ctcttttaac
     3301 agcttttaaa tattacctta tttaataaaa agagatgcca tttcattgta aaaggtgcgg
     3361 gcaatgctgc aatcctcccc atatatttaa gcaagacatc aagaggttag agagctcagg
     3421 actaaaggat tttgtctaca cagacaatct tgatcacacc tatctaaaag acaagggcgg
     3481 ttggtgcatg tttcttaaaa aaggcaagat tgcagaatgc actatctata tgataaggcc
     3541 aaagacctgc aggttatatc cttctgaact caacaacggc gactgtcggc cgatagagct
     3601 tgcgtttgat gattatttag aaaacatacc tagtcacgat tgactatatt gaaaaattca
     3661 gacattttat aaacccatta tttgtttagc ctttttatgc ctaaaaccga tgtaggagga
     3721 caagcagtca tcgaaggcgt catgatgcgc tcaaagaacg atatagtgac tgcagtcaga
     3781 aaaaaaggaa agattatcta taaagaagag aagctaaaac aaaagccaaa gtggatgggt
     3841 tttttcttca taaggggaat tgtaaacctt atcgatatgc ttgtaatagg gataaaaacc
     3901 cttaattgga gcgcttctca agcaggagaa gaagatgaag agttaagcag cacagcgata
     3961 tggataacac tcctgatagc ttttgcattc tctatcggct tgtttatggc tcttccttat
     4021 gcactgactt atctgctcgg tgtaagagag acagaaagcc cggtatggtt caatattatc
     4081 gacggaatta tcaaggccgc aatcctggtc gcatatgtct atcttatctc gctgatgaaa
     4141 gatataaggg cagtctttca ataccatggg gcggaacata aagcagtttt ctgctatgaa
     4201 gcaggaaaag agctcactat aaagaatgca aaggtatact cgacaaagca tccaaggtgc
     4261 ggaacagcat tcctgatgat tgtcatcata ataagcattt ttctcttttc attcatccct
     4321 atcttgataa acacagcata ccctttgttt gaaagccttc catggctgat aagacgaatt
     4381 accctatttg gcacaaggct tttactccta ccagtagttg caggaatatc ctacgaagct
     4441 ttaaagtttg gagcaaagca tcaaggcaac ccgctattta gggcattgac tttgccaggg
     4501 ctatggcttc agaacataac gacaaaagag cccacagaaa aacagcttga ggtgggcata
     4561 gctgccttaa agaaagtaac cgaaaatgga aatatgtcaa aaactccgct aacaaaagcg
     4621 gagcgtggct gagagcggag tttttgagca tgctcagaaa catactacat tccaatgaaa
     4681 catcaaattt atctgtgagt tcgctaatcg tcggtatgtt tctgacatat gagccagcag
     4741 aggactcttt tcttatccag agatatgtaa agaaatatgc aaaaggcata gtgcttgaca
     4801 tgggcacagg ctcaggcatc ttggcagaag aagcagctgc ctcaaagaaa gtcgtcaagg
     4861 tctttggcgt cgacaaaaac gaaaaagcca taatccactg cataagaagc caaaagagca
     4921 ggaagataac ctatgccatg tccgatcttt tctccctatt caagaattac aagccttata
     4981 tgggcatcaa gtttgacact atcttgttca acgcacccta tcttccaagc gatcccaatg
     5041 tcaaagacat ggcactggat ggtggaaaaa aaggccacga gcttatcgtc aaattcctaa
     5101 acaaagcaaa ggctttctta aagccaatgg gaaaaatcct ccttatattc tcatcgttta
     5161 caggaaagga aacccttgat aactacattt ccacgcatca ttggaaatac aaagagattg
     5221 ataaggtcca catctctttt gaagatatct acctttatct gatagaaaat gagtgagctt
     5281 acagatctta cttatcttgc gaaaggctgc agaggcatag tttatactgc agactataac
     5341 gggaaaaaag tggttgtgaa aaaagaaaaa gaaggctcaa aagcgataaa ccgattgcaa
     5401 aacgaagcaa acttccttaa aattctcaac aaagaaagca taggcccaaa attctatttc
     5461 tgcgatgaaa cctcgcttgt ctctgaatat attgaaggag agctttttcc agaatggcta
     5521 aaaaaagcaa atagcaaaga cagcctaaaa gtgatcagag atatctatgc ccaatgcttt
     5581 aagatggaca agcttaaaat aagcaaggaa gagatgcatc atccccctaa gcatatcctg
     5641 ataaggaaag gaaaaccagt gatgattgac tttgaacgat gccataaatc accagagccg
     5701 acaaatgtca ctcaattcag ctcctatatg ctctcaagga acattacaac cagtttaaac
     5761 aaaaagaata taactgtaga caaagaagga ttaatcagct catgcaagga atacaaagca
     5821 aacatgacaa gagaaaaatt caagaaggtg ctttcagcga taaaatgaat ttatcattca
     5881 atgaaaaagt atgggcattg acagcaaaga ttccaaaagg aaaagtcacc acctacaaga
     5941 ttatcgcaga aaagcttgga acaaaagctt atcgtgcagt cggtgcagcc ttagcaaaaa
     6001 acccttatgc accaaaagtc ccttgccatc gtgttgtctg ctctgatgga agggttggcg
     6061 ggtataatgg tagcatggat aataaggaga aaactagact actaaaaaaa gaaggggtaa
     6121 agataaacaa tgggaagata tcgcatttca aagctggcga aataatctct cagtaagatg
     6181 cctatcatac aaacattcgt tttataacct tttgggctgt tgcccgccta tcatactcta
     6241 ctcggtaaaa aacaacggat tggccatcat agataacaaa agaaggagag cctttaacat
     6301 ctcttggttg gccaacagaa cccactgcta tttttatatc acttttagta ctgaatcagt
     6361 tatacattcc ttataatcac ttatcatggg agttacctct ctaatgtatc tccctctttt
     6421 ttaaacaatg aaattccacc agtctatttg actggtggag tgaattgtgc tcaaaaacca
     6481 ctatctggta gaattcctct ttccaccagt ctatagcggt caaagagcgg tgactggtgg
     6541 ggcgcaacca tgaacttttt atgcttatca ctcatatttc tatcaaaagc ttaagaaaaa
     6601 ccaggaagca gacctaaacc actgaaatac ctcttgggca cttt
//
LOCUS       DASRUK010000142         4327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2285722, whole genome shotgun sequence.
ACCESSION   DASRUK010000142 DASRUK010000000
VERSION     DASRUK010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2285722"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..974
                     /locus_tag="VFF28_06275"
     CDS             24..974
                     /locus_tag="VFF28_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HZX45261.1"
                     /translation="MIASTSIMESQKLPRNISSFEHLLKLSEVFGYGLVCVKSYDAKQ
                     IVLEASSTLSKQYQKLFSSSCADHFLAGFCTGISEQILGKTMKGEIADSIIKISLAND
                     PQGELRLGNLSSITSSFSQTEASKKIIGHDLLSYNSGWFLIAGIPGHVYGNESFVLMY
                     HLLQEKYGDEFTKVLYTLGKAQSRQIGKYQIEKFGLDKNQFLNIFVQQSEMQGIGVVS
                     IEDSKDGRFLFRHERNTFALKHLNHFGKASNGIDFYMCGLLCGAVEAIFGTEYEGQEI
                     QCMAKKAPYCSYELHKSGKSAGFDPLIEQALSFSAMLPAI"
     gene            complement(949..1647)
                     /locus_tag="VFF28_06280"
     CDS             complement(949..1647)
                     /locus_tag="VFF28_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45262.1"
                     /translation="MLDEKLCRNSELLIKDYKTLLVNSWLEQIFRLRFKAYRAISAID
                     PTNYPDKKIEDKFDRLPTTWHIVLIKNRVLQGAIRIGLPCEIGLSIEEEFPLSHYFDE
                     LKKTPAECYPFIDASRLVVDPDARGMSFGTYLSYTAHRYGLDMIGARYIFSVWNPKFK
                     GSYEVGVRPLGEPKRFVLKEDQGSGNIAEHQAVASCLTRETFSKPEKIDKIDERCYII
                     RPGKNPVILQEALR"
     gene            complement(1673..3325)
                     /locus_tag="VFF28_06285"
     CDS             complement(1673..3325)
                     /locus_tag="VFF28_06285"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZX45263.1"
                     /translation="MIKIGSSSILINYVGYPHIISALQPDNGLASLAGQLVKHGHKTL
                     ILDYATADIIKRLFPAQYQQRIDTLLSALESQRQNNRPLSKELLDELKELEKKTYIYI
                     DEQTKKIAEEIDRVIKEEQPDFIAFKLFLGQSFQGSIIIADILREKNPSLKIFGGGPQ
                     VDGFMEEIFKKTQAFDALAYGEGEETILHLADYSIGKRALSSIPNLIYIEDGKVVTAP
                     LKRVEDLNGLAWPLYDEKVYPAMKGSQKMKAFLIEENRGCPYCCNFCFHPLKSGTVWR
                     KRDAKNVVDMIEDTIKKHGTNIFRFVASSPSQQLRLQIADEIIKRKLDIKWVGFERVS
                     DQPLEFYRKMKDSGCVALFFGVESGSQKILDESLGKKTKVNQIKKTIALCKEAGIYSC
                     VGIIFPCPNETKETAKETEELILEIRPDSVVISSPFMQMRTDWYNNMAKYGFKLKHPE
                     TFALDTMYFRPKFTFPPYLWDPYPHTLNGKDSDQLNKETDILAANLANNGITMGVIDD
                     SALLAVHLDMDMDKFGKASRRMIQTGDHASILQLNERINHQI"
     gene            3471..3701
                     /locus_tag="VFF28_06290"
     CDS             3471..3701
                     /locus_tag="VFF28_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45264.1"
                     /translation="MKNGNIDEAFFWREIVSAMCSYCSIDVKRFLEKLEPLREAWEIR
                     DPGYILEKIEELHANRSNFIKIEVSNILAEIK"
     gene            3772..4131
                     /locus_tag="VFF28_06295"
     CDS             3772..4131
                     /locus_tag="VFF28_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZX45265.1"
                     /translation="MKILVVDDFSGTLAVFQILLKRLTRAEVLFARSGDEALDLLGDN
                     QVDLVFIDLMLGGKLGFETAVKMKELNIAVPIVFLSTYSDKITKDRALSYGKDYIEKP
                     FATEDIEKVLAKYGFIS"
     gene            complement(4121..>4327)
                     /locus_tag="VFF28_06300"
     CDS             complement(4121..>4327)
                     /locus_tag="VFF28_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45266.1"
                     /translation="GSSSSLMKNWIIAGIVSLIVYLLIKPLLICPAAYLNPWQYFASC
                     SLFNLAIFLLVFALIYASRYVFRR"
BASE COUNT         1209 a          880 c          924 g         1314 t
ORIGIN      
        1 accaataagg caaaatacag ggtttgatag cctctacatc aatcatggaa tcccaaaaac
       61 tgccaagaaa catcagctcg tttgagcatc tactgaagtt gtcagaggtg tttggttacg
      121 gccttgtatg tgtaaaatcc tatgatgcaa agcagatagt cctggaagca agcagcacat
      181 tgtcaaagca atatcaaaag cttttttctt catcttgtgc tgaccatttt cttgcaggct
      241 tctgcacagg gatatctgaa caaatccttg gaaagacaat gaagggagaa atagcagata
      301 gcatcatcaa gataagcctc gctaacgatc cccaaggaga acttagattg ggcaacctat
      361 caagcataac cagcagtttt tcgcaaactg aagcatcaaa gaaaataata ggacatgacc
      421 ttctttctta taactccgga tggttcttga tagcaggcat acccggccat gtatatggca
      481 atgaatcttt tgttttgatg tatcatctgc tgcaagaaaa atatggagat gagtttacaa
      541 aggttttgta tactctcggc aaggcgcaaa gcaggcagat aggcaaatac cagatagaga
      601 agttcgggct tgataaaaat cagttcctta acatttttgt ccagcagtct gagatgcaag
      661 ggataggagt agtcagtatc gaggattcta aagatggcag gtttttattc aggcatgaga
      721 ggaatacttt tgcgctaaaa catctaaatc actttggcaa agcaagtaat ggcatcgatt
      781 tctacatgtg cggcctctta tgcggcgctg ttgaagcgat atttggaaca gagtatgaag
      841 ggcaagagat acagtgcatg gctaagaaag ctccttattg ctcttatgaa ttgcataagt
      901 caggcaaaag cgcgggcttt gatccactga tagagcaagc cttgagcttt agcgcaatgc
      961 ttcctgcaat ataaccgggt ttttaccagg ccttattata taacatcttt cgtcaatctt
     1021 gtctatcttc tcaggcttac taaatgtctc tcttgtgagg caggaggcga cagcttggtg
     1081 ttctgcaatg ttaccgcttc cttggtcttc ttttaataca aaccttttgg gttcgcctaa
     1141 tggacgcacg ccaacttcat aagagccctt aaattttgga ttccatactg aaaagatata
     1201 ccttgcccct atcatatcca ggccatacct atgcgcagta taggatagat atgttccaaa
     1261 tgacatacct cttgcatcag ggtccacaac aagccggctt gcatctataa acggatagca
     1321 ttcggcaggt gtttttttga gctcatcaaa gtaatgactt agaggaaact cttcttcaat
     1381 cgacaaacct atctcgcaag gcagcccaat ccttattgct ccctgaagga ctctgttttt
     1441 tatcaatact atgtgccaag tggtgggcag cctgtcaaac ttatcctcta tcttcttatc
     1501 aggataattt gttggatcaa tcgcacttat tgccctgtaa gccttgaacc tcaatctgaa
     1561 tatctgctca agccatgagt tgacaagaag tgtcttgtaa tctttgataa gaagctcgct
     1621 gttgcggcat agtttttcat ctagcaaaat tttctccacc tcttttttat ctctatatct
     1681 gatggtttat ccgttcgttc agctgaagga tggaagcatg atcacctgtc tgtatcatcc
     1741 ttctacttgc ttttccaaac ttatccatgt ccatatcaag atggactgca agaagcgcag
     1801 agtcatctat cacacccata gttatcccgt tgtttgcaag gtttgcagca agaatatctg
     1861 tctctttgtt caactggtct gagtccttgc cgttaagggt atgaggatag ggatcccaaa
     1921 gataaggggg aaaggtaaac ttaggccgaa agtacatagt atcaagagca aatgtctcag
     1981 gatgttttag cttgaagcca tattttgcca tgttgttgta ccaatctgtc ctcatctgca
     2041 taaatgggga tgagatgaca acagagtcag gccttatctc cagtatcaac tcttctgtct
     2101 cttttgcagt ctcttttgtc tcgtttgggc agggaaagat tattcccaca cagctataga
     2161 tccctgcttc tttgcacaaa gcaattgttt tttttatctg gtttaccttg gtcttctttc
     2221 caagagactc atcaagaatc ttttggctgc ctgattcgac accgaagaat aaagctacac
     2281 agcctgagtc tttcatcttt ctatagaatt caagcggctg gtctgagaca cgttcaaaac
     2341 cgacccattt gatatcaagt tttcttttta ttatctcgtc tgctatctgt aaccttaatt
     2401 gctgagaagg gcttgatgcg acaaagcgaa atatgtttgt cccatgcttc tttatcgtat
     2461 cctctatcat atcaacgacg ttttttgcat ccctttttct ccaaacagtt ccacttttta
     2521 gcggatggaa acaaaaattg cagcaataag ggcatcccct gttttcttct atcaagaatg
     2581 ctttcatctt ctggctgcct ttcattgcag gatacacctt ttcatcatat aaaggccatg
     2641 caaggccatt gagatcttca acccttttca ggggcgctgt tacaactttt ccatcttcta
     2701 tatagatcag gttgggtata gaagacaagg ctctctttcc gatggagtag tctgcaagat
     2761 ggagtatagt ctcttcgcct tccccatagg caagtgcatc aaaagcctgt gtttttttga
     2821 atatctcctc cataaatcca tcgacctgag ggcctccacc aaatatcttt aatgaaggat
     2881 tcttttctct taggatgtct gcaataatta tggaaccctg aaaggattgg ccaagaaata
     2941 gcttgaaggc gataaaatca ggctgttctt ctttaatcac tcggtcgatt tcttcggcta
     3001 ttttttttgt ctgttcatcg atatagatgt atgtcttttt ttcgagctct tttagttcgt
     3061 ctagcaactc tttacttaat ggcctgttat tctgcctttg gctttccaat gcagagagaa
     3121 gagtgtctat cctttgttga tattgtgcgg gaaaaagccg cttaatgata tcagctgtcg
     3181 cataatcaag gatcagggtt ttatggccat gcttgacaag ctggcctgca agggatgcaa
     3241 ggccgttgtc aggctggagc gcactgatga tatggggata accgacgtaa ttgatgagga
     3301 tgctgctgct tcctattttt atcacctctt atgagccttg ccttgagcga ggtgtaataa
     3361 acattatcga gggtatctgc tgcaaagagg tgcaatcctg acagagttag ccatgtatct
     3421 ggcagaaatc agaaagaatc attaaaaagc agggcatttt ttgcaatact atgaaaaatg
     3481 gcaatatcga cgaggcattc ttttggaggg aaattgtcag cgctatgtgc agctattgca
     3541 gtatcgacgt caaacggttt cttgagaagc tagagccttt gagggaagct tgggaaataa
     3601 gggacccggg ctatatcctt gaaaagatag aagaactgca tgcaaacagg tctaatttca
     3661 taaaaatcga agtttcaaac attctggcag agataaaata gtgtttgctt cctttaagca
     3721 tacggccaga tgcctgcaag ggcatttggc tgtttttata cgagggataa tgtgaaaata
     3781 ctggtggttg atgattttag tggaacactt gctgtcttcc agatattgct taaaaggctt
     3841 acccgagcag aggtcctttt tgcaaggtct ggtgatgagg cattggattt acttggagat
     3901 aatcaggtag atcttgtatt tatcgacctt atgcttggag gcaagctggg gtttgagaca
     3961 gctgtgaaga tgaaggaact aaatatcgct gttcctatcg tatttctgtc aacctattct
     4021 gacaaaatca ccaaggatag ggccctttct tacggcaagg attatatcga gaagcctttt
     4081 gccactgaag atatagagaa agtgcttgca aaatatggat ttatctccta aaaacatatc
     4141 tcgaggcata tatcaaggca aagacaagaa ggaatatagc aaggttaaac aggctgcagg
     4201 aggcaaaata ctgccaggga ttaaggtatg ctgcagggca tatgagcagg ggttttatca
     4261 ataggtaaac tatcaaagag actatacctg ctataatcca gttcttcata agactacttg
     4321 aagatcc
//
LOCUS       DASRUK010000143         2431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1304599, whole genome shotgun sequence.
ACCESSION   DASRUK010000143 DASRUK010000000
VERSION     DASRUK010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304599"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFF28_06305"
     CDS             <1..193
                     /locus_tag="VFF28_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45267.1"
                     /translation="RDKLCKDSNFCVEFASDEEARRSLELVRFANVHTQKPLTEEELK
                     FVVKYPAVAAKIMTVTPQF"
     gene            complement(435..881)
                     /locus_tag="VFF28_06310"
     CDS             complement(435..881)
                     /locus_tag="VFF28_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45268.1"
                     /translation="MRHLKPFESFIKEEIVRKISVDTQRAKSLIIESERKMRSINLQL
                     EKIGIVDDNTNDYVEQCFDSLMNLVRAKLYMIGYSASGQGAHEAEVSYLRELGFSEKD
                     VQFADQLRYFRNGILYYGTSLDKEYADKVLEFTKTVHSKLKKFLEG"
     gene            complement(878..1414)
                     /locus_tag="VFF28_06315"
     CDS             complement(878..1414)
                     /locus_tag="VFF28_06315"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZX45269.1"
                     /translation="MDIYKIKWTTLEQEIFSLLCIRAGEKLSQRELAKILRVSPTAVS
                     NSMKKLRGSNLVKVEKTKTINFVSFNRDHQAAGDLKRVENLKNMYLSGLSDYLEHELA
                     GATVILFGSYSRGEDTVASDIDIAVIGRKGKPLDIEEHEKKLFRKINVNFFDSWKIEQ
                     NLRNNILNGIVLHGGIEL"
     gene            complement(1457..2248)
                     /locus_tag="VFF28_06320"
     CDS             complement(1457..2248)
                     /locus_tag="VFF28_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008085101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZX45270.1"
                     /translation="MDIDLSSNEPYLKKLSEELRLRKYSPQTEKTYLHIIRSFLAKGC
                     QPKDHLLSLTDKSRSLMRTTYFALKFFYENVLSQKFDEKIPLAKNSSKLPIVLSKEEI
                     ERLMNTTVNIKHRFIILLLYYTGIRVGELTGLDWKDMDLDRDIIHLKTTKGSKDRVIF
                     LHPMVKEYIQKFYPKAEGLVFTVNGKKYSKRTIQAMIKTAQIRAGISKRVTPHTLRHS
                     FATHLLEGGADIRHIQMLLGHSNLQTTQVYTHVANKDIKNLAKLI"
BASE COUNT          617 a          507 c          476 g          831 t
ORIGIN      
        1 gagggacaag ctatgcaagg actctaattt ctgcgtcgag tttgcaagcg atgaggaagc
       61 aagaagaagc ctggaacttg tgcgctttgc aaatgtccat actcaaaagc cgcttactga
      121 ggaagagctt aagtttgtgg tgaagtatcc ggcggttgct gcaaagatca tgactgttac
      181 gcctcagttc taggtaataa ttataattag atgaatattg gaaaactttt gctatctatc
      241 ggcttatgcc tgtctgcagg catcatcggt tcggtcttca cccaatcatc tgtcagtatg
      301 aggggctaag caagccgtgg ttcaattcgc caagctatgt ttttgggcct gtctggacgg
      361 ctcttatgtt aaactagaat catataagcc ttgccaggtt ctttatgtcc ttatttggcg
      421 tgatgaccga acatctatcc ttcaagaaat ttcttaagct ttgagtggac agtttttgta
      481 aattcgagaa ctttgtctgc atattctttg tcaagacttg tgccataata tagtatgcca
      541 tttctaaagt atctaagctg gtctgcgaat tgcacgtcct tttcagaaaa tcccagctct
      601 ctaagatagg aaacctctgc ttcatgagct ccctggccac ttgcagagta gccaatcata
      661 tataatttcg ctcgcaccag gttcataagg gaatcgaaac attgctcaac atagtcgtta
      721 gtattgtcat caacaatgcc gattttttcg agctgcaggt ttatcgaccg catctttctt
      781 tctgattcga ttataaggct ttttgccctt tgcgtgtcca cagagatctt cctaactatc
      841 tcttccttga tgaatgactc aaagggcttt aggtgcctca tagttcgata cctccatgaa
      901 gcacaatgcc atttaaaata ttatttctaa gattttgctc aatcttccag ctatcaaaaa
      961 aattgacgtt aattttacga aaaagttttt tttcgtgttc ttcaatgtcc agtggcttac
     1021 cttttctacc tatcacggcg atgtctatgt cagatgcaac tgtgtcttcc cctcttgaat
     1081 aactgccaaa taatattaca gtggcacctg caagctcatg ctcgagataa tccgacagcc
     1141 ctgaaagata catgtttttt aagttctcta cccttttaag atcccctgct gcctggtggt
     1201 ccctattaaa ggagacaaaa tttatagtct tagtcttctc aaccttaacc agattgctgc
     1261 ctctaagttt tttcatggag tttgagacgg cagtgggaga taccctgaga atctttgcca
     1321 attccctctg gctaagcttt tcgccagccc ttatgcaaag caaggaaaat atctcctgtt
     1381 ccagtgttgt ccatttaatt ttatatatgt ccattttaat tgacatgtgt acagttaagt
     1441 ttataatact tttgatttaa ataagtttgg ccagattctt tatgtcttta ttggcaacat
     1501 gggtgtagac ctgggttgtc tgcaggttgg aatggccaag gagcatctgg atatgcctga
     1561 tgtctgcgcc gccttccaat agatgagtgg caaagctgtg ccttaaggtg tggggagtga
     1621 ctctcttact tattcctgcc ctaatctgtg cagtttttat catcgcctga attgtccttt
     1681 tactatactt cttaccattg actgtgaaaa ccaatccttc agcctttgga tagaattttt
     1741 gtatatattc cttgaccatg gggtggagaa agatcaccct atccttgctt cctttggtgg
     1801 tctttagatg gattatatcc ctgtctaagt ccatatcttt ccaatcaagc cctgttagct
     1861 cacccaccct tatacctgtg taatataaca gcaatatgat gaaacggtgt ttgatgttga
     1921 ctgttgtgtt catcaaccgc tcaatctctt ctttgctaag tacaattgga agctttgagc
     1981 tgttctttgc caaggggatc ttctcatcaa atttttggct taatacattc tcgtaaaaga
     2041 attttagagc aaagtaagtt gttctcatca aggagcggct tttatctgtc aggcttaaga
     2101 gatgatcttt aggctggcag cccttggcaa gaaaactcct tattatgtga aggtaagttt
     2161 tttctgtctg cggcgaatac tttcttagcc ttaactcttc cgaaagcttt tttaggtaag
     2221 gttcattact cgataggtca atatccatat ggtgaggtat ttaactaaaa gtatataaaa
     2281 tttgcggttt aacgtataag gtaagctcag attgaagctc ttagctcaga aaatgtatct
     2341 aatcctcttt gtctccacgt ggttatcatt gtcattatag tttctactat tcttgctccc
     2401 tttttattgc gcagtgaacc tattatcttc c
//
LOCUS       DASRUK010000144         7695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_288888, whole genome shotgun sequence.
ACCESSION   DASRUK010000144 DASRUK010000000
VERSION     DASRUK010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 7695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7695
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288888"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VFF28_06325"
     CDS             complement(<1..485)
                     /locus_tag="VFF28_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45271.1"
                     /translation="MKNLTLAKVGGAAYLALNAAQHIAYNLQHSISNLPAQYLSGKAA
                     DACELAAPGAAALALHSAASSIEDCLTPGRLSALRGSLPLAAATLGVAAILNQHGHYL
                     GIPEGLGVMETAQHLLLNYRDAVIDLCTGKDPNGAYLTGALVTASSAGRLTKNFLGAV
                     M"
     gene            complement(482..757)
                     /locus_tag="VFF28_06330"
     CDS             complement(482..757)
                     /locus_tag="VFF28_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45272.1"
                     /translation="MNNPVKKLAAAGVLGVAYIMGGCSTDSGFHVTVLDVKGGSQAAL
                     YRKGDDRSASDREIFQRDCSPFGDSNISDQSGFLKHYRLGEIGGDKQ"
     gene            complement(852..2126)
                     /locus_tag="VFF28_06335"
     CDS             complement(852..2126)
                     /locus_tag="VFF28_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45273.1"
                     /translation="MLNNILGRNSLRQAVIYLSILPLLAACQPARGLDKKVNDKVLSA
                     NLTENISAEGRVTGEYAAVMADGARTGGGNEASLGDFYVNIAGNLEHSTNSHSLGFFV
                     DYVPRGSVLDFNTSNSNSHNSNRLRADGFLNRPDLLSHVHLDKRLGDNKLSVSLGGKK
                     FAGLETLDGSPTDYYAAQLSPLAISQHYDKGIQIAYSAPNRFNLDLGIIDGDWQMGEP
                     SVFRHHDSRANSYPGFSFNIAWEPFSSKRFGALNLEGSALIDDIGSNGGQKTYNNNLI
                     IAASYSAPGLWKDSLLKIRAFAGNFERGKTWGPNSERDWPAEMTNFFGLEGSIGNIPL
                     GDAGSLAAYVGYARMNHESGEPAGTIWKSRNTSHEGQYQSGLRWKEPYGIKHLTLNFS
                     AGKRSMDGLPDWHINDKKEQYIGFLGAEIDFK"
     gene            2241..3527
                     /locus_tag="VFF28_06340"
     CDS             2241..3527
                     /locus_tag="VFF28_06340"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HZX45274.1"
                     /translation="MTQINPYENARQIISEAADILNIEPWIKEVLLMTHRELHVTFPI
                     EMDDCTVKVFNGFRVQHNHMRGPFKGGIRYHWDVNLDEIRALATWMTIKCSVMDLPFG
                     GAKGGVICNPKDMSEKELETMTRAFTRRISPILGSNIDIPAPDVYTTPKVMAWIADEY
                     ARITGNKDIAVVTGKPIDIGGSEGRNEATGLGGLYAIKQAIKERASNGIESLKGKRIV
                     VQGFGNVGGIFAELAHNEGAKIIAVSDSKGAVINNHGIDIGKLTIHKKKVGCVLEFPA
                     SKPSTNEEILELDCDILVPAALENVITMENADRIKAKVILELANGPVSASADSVLNKK
                     GIIVIPDVLANAGGVTVSYFEWKQDLEKQHWKKNEVYAKLEEKMNSNTSAVIDAARKY
                     KVSLRLGAYILAISRITEKAKIELDKDHYHCFRDYL"
     gene            3576..4751
                     /locus_tag="VFF28_06345"
     CDS             3576..4751
                     /locus_tag="VFF28_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZX45275.1"
                     /translation="MKIEASERVKSIGSYAFADVDNEVAKLKKQGITPIDFGVGDPKD
                     PTPGLIRNYCKRAVDKRMSAGYPSYIGEQDYREAIASWTKKRFNVTLDANKEVTSSIG
                     AKEAVFNLHEGFVNPGDYVIMPNPGYPPYERGTLFAEGRSYHYPLTKENDFLPDLEKI
                     PKDIIKKSKLMWINYPNNPTGATITKEKLKEVIDFGKDNNIIIGSDECYTEIYFEEKP
                     LSILELTKEGVFSVQSLSKRSAMTTYRVGWVSGDENIISIFKKVKTNIDSGTATFIQD
                     AATAALLDEKHVEEFRELYRRKRDMVVNALTSIGLPDCSPAATLYIWQKGPSGMSSVD
                     FSKKLLEKDVAIVTTPGSWISKEVDGVNPGEGYVRFALVPPIAECREAAKRIRKLEI"
     gene            4813..5121
                     /locus_tag="VFF28_06350"
     CDS             4813..5121
                     /locus_tag="VFF28_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45276.1"
                     /translation="MNDQYTNYYVYTRHAIGGTNYTASYTTIAGLVGLAIIRENGSKR
                     LAGNLHFLPLASHLLAQAQGSHFDPLEEIAKKALRQWGISTNVQWTNEGDLDRFLNGE
                     "
     gene            5123..5941
                     /locus_tag="VFF28_06355"
     CDS             5123..5941
                     /locus_tag="VFF28_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HZX45277.1"
                     /translation="MGKRLIDILGISQGDLEKRLIATDGKQGKSPFEDPSIVRPSKIN
                     NLYERLGLENFCEDQESIKSAYRRQAVVWHPDKNPRYFDDANFEFSKITEAYETLSDS
                     GKKSDYDDQLREKLRQNGKNEDFQQNPTNALKSSRLSRSYVRFPDIDGNLKPEQKAIL
                     DYLDFLVDIGQITLSGSTGMNMQLQPYRCEYCKETISPRGLVISYRHKTMAFGESYAH
                     AFLAHGSRAFLTEFLKSLPGQYGYAMAAVDTLCKELGIDVSQRTLNNPHNKLLK"
     gene            5996..7558
                     /gene="lysS"
                     /locus_tag="VFF28_06360"
     CDS             5996..7558
                     /gene="lysS"
                     /locus_tag="VFF28_06360"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HZX45278.1"
                     /translation="MAESMHWADQTARRVVAQKGEKERYTVAAGITPSGTVHIGNFRE
                     MITVDLVYRALKDLGKNTRFIYSWDDYDVFRKVPKNMPKQDLLTTFLRKPIVDTPDTF
                     GCHSSYAEHNEKEVEDVIPIVGISPDFKYQSKKYRGCEYKDEIKFALNNKEKVRVILD
                     KFREEPLPKEWYPVSIYCEKCNRDDTEVKSYDGNYTLAYTCQCGYEGNINFSKKGNVK
                     LPWRLDWPMRWHYEGVDFEPGGKEHSTEGGSYTTAKLIVKELYNQDAPVYQKYDFIIL
                     KGVGGKMSSSSGNVITLKDTLAVYEPAIIRYLFAGTRPETEFSISFDLDVLKIYEDFD
                     KCERIYYKKEEVAEKEFEKQKRIYELSCIDKPSKEMPFQPSLRHLCNMVQVYEDDYSQ
                     VIASYSLKEKSDIDRLKMRAVCASNWLKKYAPDDMRFTVKKEVTIKLEGKEKEAVKNL
                     VSYMESKSELDEKLLYEEIYNISRAAGVEPKDFFKVCYQILIGKEKGPKLAGFILIIG
                     KDRVLDLLKQAI"
BASE COUNT         2404 a         1533 c         1678 g         2080 t
ORIGIN      
        1 ttcatgacag cgccaaggaa attctttgtc agccttcctg ctgacgatgc tgtgactaaa
       61 gcgcctgtca gataagctcc atttgggtcc tttcctgtgc atagatcaat gacagcatcc
      121 ctgtaattta acagaagatg ctgtgcagtt tccatcactc caaggccttc tggtatccca
      181 agatagtgtc catgttggtt aagtattgct gccactccaa gtgttgctgc cgcaagcggc
      241 aagcttcctc ttaaagcgct aagccttcca ggggtcaggc aatcttctat ggaacttgct
      301 gcactgtgca aagcaagagc tgctgctcca ggtgctgcaa gttcgcaagc atctgccgct
      361 tttcctgaga ggtactgagc aggaagatta ctgatagaat gctgaaggtt gtaagcaata
      421 tgctgtgctg cattaagtgc cagataggca gctccgccaa cttttgctaa agttaagttt
      481 ttcattgttt atctcctcct atttcaccaa gcctatagtg tttcaaaaac ccgctttggt
      541 cgctgatgtt tgaatcgcca aaagggctgc agtctctttg aaatatctcc ctgtcgcttg
      601 ccgacctgtc atcgcctttc ctgtacaaag cagcctgact gcctcctttg acatcaagca
      661 ctgtcacatg gaagcctgaa tcagtcgagc atccgcccat gatgtaagct actcccagca
      721 cacctgctgc tgccaacttt tttactggat tattcatttt ttcaccttgt gtaagtaata
      781 aaaaatgtga gaaaaagctt agactaaagc tttgcatgac atagggtaag ctatatgcat
      841 gacataggtc attacttgaa gtctatctca gctccaagaa atcctatgta ttgctccttt
      901 ttgtcgttga tatgccagtc aggtaagccg tccatgcttc ttttgcctgc agaaaagtta
      961 agggtcagat gttttatccc atacggctcc ttccatctaa gaccagattg gtattggcct
     1021 tcatggctgg tatttctgct cttccatatc gttcctgctg gctctccgga ctcatggttc
     1081 atacgcgcat agcctacata cgctgccagg cttccagcat ctccaagcgg gatatttcct
     1141 atcgatcctt caaggccgaa aaaattggtc atctcagcag gccagtccct ttctgaatta
     1201 ggcccccatg tcttgcccct ctcaaaattc cctgcaaacg cccttatctt aagtagtgaa
     1261 tctttccata atccaggcgc agaatagctt gctgcaatta taaggttatt gttgtatgtc
     1321 ttttgccctc catttgagcc gatatcgtcg attagggctg atccctccag atttagtgct
     1381 ccaaatcttt tgcttgagaa aggttcccag gcgatattaa atgagaatcc aggataagag
     1441 ttcgcccttg aatcatgatg cctaaataca cttggctctc ccatctgcca atctccatct
     1501 attatcccca agtcaaggtt aaatctgttt ggtgctgagt atgctatctg tatcccttta
     1561 tcatagtgct gactgatagc taaaggggat aactgcgcag cataatagtc tgtcgggctg
     1621 ccatccagcg tttcaaggcc tgcaaatttt ttgcccccta gacttacgct aagcttgtta
     1681 tcacctaatc ttttatcaag atgtacatgg gagagaagat cgggtctgtt caaaaaaccg
     1741 tctgccctca gcctgttaga attgtggctg ttggaattgc tggtattgaa atcaaggaca
     1801 cttcctcttg gaacgtaatc tacaaaaaat ccaagcgagt gagaatttgt gctgtgctca
     1861 agattccctg caatgtttac gtaaaaatcg ccgagcgaag cctcattccc gccgccagtc
     1921 cttgcaccat cagccatgac tgcggcatat tcacctgtga ctcttccttc agcagatatg
     1981 ttctctgtta gattagcgct caaaacctta tcattgacct ttttatccaa acctcttgct
     2041 ggctggcaag cagccaatag cggtaagatg gataggtata tcactgcctg tctaagcgaa
     2101 tttcttccta agatattatt aagcatggtt tttgtggttt ttcagcgcat tattaatcat
     2161 tgattgacat agggcatctt tttacaaagg acattagcca atagcaaatc tttatatctg
     2221 cctttacctt atcagtcctg atgacccaaa ttaatcctta cgagaatgcc cgtcagataa
     2281 tcagcgaagc agcagacatc cttaacatcg agccatggat aaaagaagtc cttcttatga
     2341 cccacaggga gctccatgtc acattcccta ttgagatgga cgattgcact gtcaaagttt
     2401 tcaacggctt tagggtccag cataatcaca tgaggggtcc ctttaaagga gggataagat
     2461 atcattggga tgtcaacctt gacgagataa gggcacttgc tacatggatg actataaaat
     2521 gctctgtgat ggacctgcca tttggtgggg caaaaggagg agtcatctgc aacccaaaag
     2581 atatgtcaga aaaagagctt gagaccatga caagggcatt cacccgaagg ataagcccta
     2641 tacttggctc taatattgac attccagcgc cggatgtcta caccacccct aaagttatgg
     2701 catggatcgc tgatgaatat gcccggatca caggcaataa ggacattgct gttgtgactg
     2761 gaaagcccat cgacattggc ggctctgagg gaaggaacga agcgactggc ttgggcgggt
     2821 tgtatgcaat caagcaagcc ataaaagaga gagcgtcgaa tgggatagaa tctctaaaag
     2881 gaaaaaggat agtagtgcag ggctttggaa atgtaggcgg gatatttgca gagcttgctc
     2941 ataatgaagg tgcaaaaata atagcagtgt cagactctaa aggggcagtc attaataatc
     3001 acgggattga tataggcaaa ctgaccattc ataagaaaaa ggtaggttgt gtccttgagt
     3061 ttcccgcaag caagccttca acaaatgagg aaatattgga gctggattgc gatatacttg
     3121 ttccagcagc ccttgaaaat gtgataacta tggagaacgc agacaggata aaggcaaagg
     3181 ttatcttgga gctggcaaac gggcctgtgt cggcaagcgc tgattctgtg cttaacaaaa
     3241 aagggattat cgtcatcccg gacgttctag ccaatgcagg cggggtcaca gtctcttatt
     3301 ttgaatggaa gcaggacctt gaaaagcagc actggaaaaa gaatgaagtc tatgccaaac
     3361 ttgaagagaa gatgaactca aatacatctg cagtgataga tgcagcccgg aaatataaag
     3421 tcagcctaag gcttggagcc tatattcttg caatctccag aataaccgaa aaggcaaaga
     3481 tcgagcttga taaggatcat taccattgct ttagggatta tttgtagctt ttttctacat
     3541 aagatttata aattacttat ttattgccta taggtatgaa aatagaagcc tcagaaaggg
     3601 tgaagagcat aggaagctat gcgtttgctg atgttgacaa tgaagttgca aagctaaaaa
     3661 agcaggggat aactccaata gatttcggcg ttggcgatcc aaaagacccg acacctggcc
     3721 ttataaggaa ctactgtaaa agggcagttg acaaaaggat gagtgcaggc tatccaagct
     3781 atattggcga gcaggattat cgggaagcca tcgcaagctg gacaaagaaa agattcaatg
     3841 ttacccttga cgctaacaag gaggtaacct cttcaatagg cgcaaaggaa gcagtattca
     3901 acctgcatga aggttttgtc aatcctggcg attatgtgat aatgccaaat cctggttatc
     3961 caccttatga gagaggcact ttgtttgcag aaggaagatc ataccattat ccgctgacta
     4021 aagaaaatga tttcttgcct gacttagaaa agatacccaa agatatcatt aagaaatcaa
     4081 agcttatgtg gataaattat ccaaataatc ctaccggagc gaccataacc aaagaaaagc
     4141 tcaaagaagt tatcgacttt ggaaaggata acaacatcat tatcggctca gacgaatgtt
     4201 atactgagat ctattttgaa gaaaagccat tatccatctt agagttgaca aaagaaggtg
     4261 tcttttctgt gcagagcttg tctaaaaggt ctgcaatgac cacatacagg gtgggttggg
     4321 tatcaggcga tgaaaacatt atctctatct ttaagaaagt aaagacaaat atcgattcag
     4381 gtactgcaac ttttatccag gatgcagcca cagcagccct gcttgatgaa aagcatgttg
     4441 aagaattcag ggagctttat aggaggaaga gagatatggt ggtcaatgct ttgacatcga
     4501 ttggacttcc tgactgttct cctgctgcta ccctctatat ctggcagaaa gggccttctg
     4561 gcatgtcctc tgtcgacttc tcgaagaaac tgcttgagaa ggatgttgct atcgtaacaa
     4621 cgccgggctc gtggatttct aaggaagtcg atggcgttaa tccaggcgaa gggtatgtaa
     4681 ggtttgcact cgttcctccg atcgcagagt gcagggaagc ggcaaagagg attaggaagt
     4741 tagagatttg attattacta actagtagca attagcgtaa tatttataaa ggttaatgaa
     4801 attctttcct gtatgaacga ccaatataca aattattatg tctatacaag gcatgctatt
     4861 ggtggaacaa actatacagc tagctataca actattgccg gattagttgg cttggcaatc
     4921 atccgagaaa atggcagtaa gaggttagct ggaaacctgc actttttgcc gcttgcatct
     4981 cacttactag cacaagcaca aggtagccat tttgatccat tggaagaaat cgctaaaaag
     5041 gcattgagac agtggggaat tagcacaaac gtccaatgga caaacgaagg agatctagac
     5101 agatttctta atggtgaata acttgggaaa gagattaatt gatatattag gcattagcca
     5161 gggagattta gagaaaagat tgattgctac agatggaaag caaggtaaat ctccatttga
     5221 ggatccttct attgtaaggc caagtaaaat taataacctt tatgaaagac taggattaga
     5281 gaatttctgt gaagatcagg agagcataaa gtcagcttat agaagacaag cagtagtctg
     5341 gcatcctgat aaaaacccac gctattttga tgatgcaaac tttgaatttt ccaagataac
     5401 tgaagcctac gaaacgcttt ctgattctgg gaaaaaatca gactatgatg accaactaag
     5461 ggaaaaactt cggcaaaatg gaaaaaacga agattttcag caaaatccca caaatgctct
     5521 caaatcttca agactttcaa gaagctatgt taggtttcca gatatcgatg ggaatttaaa
     5581 gcctgaacaa aaagcaattt tggattacct cgattttctt gttgatattg gtcaaattac
     5641 actctcaggg tcaaccggaa tgaatatgca gttgcaacct tatcggtgtg aatactgcaa
     5701 agaaacaatt tctcccagag gattagttat ctcgtataga cataagacta tggcatttgg
     5761 agaaagctat gcacatgcct ttttggcgca tggttcaaga gcgtttttaa ccgaattttt
     5821 aaaaagctta ccgggtcaat atggatacgc aatggctgct gttgatactc tttgtaagga
     5881 attaggaatc gatgtatctc aaagaacttt aaacaacccc cataataaac ttttgaaata
     5941 attactccct tggcaaacaa caatctttat aaaaaacctt aaatctttct ttattatggc
     6001 tgaatcgatg cattgggcag accagactgc aagaagggtt gttgcccaaa agggagaaaa
     6061 agaaagatac actgtagctg caggcatcac accaagcggt actgtgcata tcggcaattt
     6121 cagagagatg ataactgtcg atctggtgta tagagcgcta aaagatcttg ggaaaaatac
     6181 aagattcatc tattcctggg acgactatga cgtgtttaga aaagtcccaa agaacatgcc
     6241 caagcaggat cttctcacaa ctttcttaag aaaacccata gtcgatacac cagatacttt
     6301 tggctgtcat tcaagctatg cagaacataa tgaaaaagaa gtcgaggatg tcatacctat
     6361 cgtcggcatc tctcctgact ttaagtacca gagcaagaaa tacaggggct gtgaatataa
     6421 agatgaaatc aagtttgctt taaacaataa agagaaggtt agagtgatac tggacaaatt
     6481 cagagaggag ccgcttccca aggaatggta tccggtctca atctattgcg aaaaatgcaa
     6541 cagggatgat acagaagtta aatcatatga tggcaactat actctagctt atacttgtca
     6601 atgtggctat gaaggaaata tcaacttcag caagaaagga aatgttaagc ttccatggag
     6661 acttgactgg cccatgagat ggcactatga gggcgttgac tttgagccag gaggaaaaga
     6721 gcacagcaca gaaggcggaa gctacactac agcaaagttg atagtaaaag aactatacaa
     6781 ccaagacgcc cctgtctacc aaaagtatga cttcataatc ctaaaaggcg ttggcggaaa
     6841 aatgagcagc tcatctggca atgttatcac acttaaagat actcttgcag tttatgagcc
     6901 agcaatcatc agatatttat ttgctggaac acgaccagag actgagttct caatttcctt
     6961 tgacttggat gtcttaaaga tctatgaaga ttttgataag tgtgaaagga tatattacaa
     7021 gaaagaagag gttgctgaaa aagaatttga gaagcaaaaa aggatctacg agctttcttg
     7081 tatagataag ccctctaagg aaatgccttt ccagccttca ctaaggcact tatgtaacat
     7141 ggtccaggtg tatgaggatg attattcaca agtcattgca agttacagcc taaaagaaaa
     7201 gtcagacata gataggctca agatgagagc agtctgcgct tcaaactggc tgaagaaata
     7261 tgcacccgac gatatgaggt ttactgttaa aaaagaagtc accataaagc ttgaaggcaa
     7321 agaaaaagaa gcagtaaaaa accttgtaag ctatatggaa agcaaaagcg agcttgacga
     7381 aaagcttctc tatgaagaga tatacaacat atcaagggca gcaggggtcg agccaaagga
     7441 cttctttaaa gtctgctacc agatacttat cggcaaggaa aaaggtccaa agcttgcggg
     7501 atttatattg ataattggaa aagatagagt tcttgatttg ttaaaacaag caatataatt
     7561 tgttattatt ctatcatcat atttttaaag ctgtaaaact aaaattatct tatagtaaat
     7621 taaattgaat atttctcatt aaatggaata agagatagaa ctttttgttt gttgtaccaa
     7681 agacttctga agaat
//
LOCUS       DASRUK010000145         4039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1951391, whole genome shotgun sequence.
ACCESSION   DASRUK010000145 DASRUK010000000
VERSION     DASRUK010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1951391"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..1164
                     /locus_tag="VFF28_06365"
                     /pseudo
     CDS             65..1164
                     /locus_tag="VFF28_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611147.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1303..1554
                     /locus_tag="VFF28_06370"
     CDS             1303..1554
                     /locus_tag="VFF28_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45279.1"
                     /translation="MAKITDRQVIVSKGTHKPTIRRPLANIERAAIYAYTCLNLLVSQ
                     RTREIGMDEEHIKVTKTDISYHSEEAYRQAKFLFKMKVQ"
     gene            complement(1556..1921)
                     /gene="hisI"
                     /locus_tag="VFF28_06375"
     CDS             complement(1556..1921)
                     /gene="hisI"
                     /locus_tag="VFF28_06375"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HZX45280.1"
                     /translation="MKLDFSKLSGLVPTIIQDYKTNKVLMVGFMNEESYKKTLETGNV
                     WYYSRTRNKLWMKGETSGNVQKVKEIFVDCDNDTLLIKAEQIGKAACHEGYESCFFRD
                     IKGKVVDKKVFDPKTVYKK"
     gene            complement(1974..2330)
                     /locus_tag="VFF28_06380"
     CDS             complement(1974..2330)
                     /locus_tag="VFF28_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45281.1"
                     /translation="MTYKQLRDSSNLKFFHRRHTIDTTIMALSAAVGGFLTHAGFAAR
                     DSAKTLLKLSTFISSPEAEQFIKKASGEYVISYIAWGMALVAYGLAVVEYKKVIDNPI
                     EYINDTEGYLLRKRKE"
     gene            complement(2388..3239)
                     /locus_tag="VFF28_06385"
     CDS             complement(2388..3239)
                     /locus_tag="VFF28_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZX45282.1"
                     /translation="MAAVYYVIGIVALFIFFIGIRVVRPTERALVERLGKYEKFAGPG
                     FHWVIPVIDRMIKVNTTENMVNAEPQEIITNDNLNARVDAQVYFRVKSDEESIKASQY
                     NVNEYQVQIVSLARTTLRNIIGTLTLKSANSERDRINDELMKTLKEETKSWGIEVVRT
                     ELKEIDPPKDVQDTMNKVVKAENEKIASKDLAEAREMQADGERRASIKEAEGIKQATI
                     LKAEGEAKAFEMISKSFVGNAQLLKKLEVTQASLENNTKIVLTEKGITPNIIMGDIPI
                     RIKGEPK"
     gene            3344..>4039
                     /locus_tag="VFF28_06390"
     CDS             3344..>4039
                     /locus_tag="VFF28_06390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZX45283.1"
                     /translation="MKIAQISPLHESVPPMLYGGTERDVHYLTEELVKMGHEVTLYAS
                     GDSKTSAELKPMCKKSLRMDKNCVDPVPDHLYMAERVCQDSGDFDIIHSHLEYLGFPM
                     LRRAKAPSVTTLHGRLDYPNYAGLFMEFRDMPLISISNSQRGPLPWANWIDTVYHGIP
                     KDIYELNEGKGEYLAFLGRISPEKGVDKAIKIAERANTPLRIAAKVGKEDREYFEEVI
                     MPLIDESNAEFIGE"
BASE COUNT         1218 a          807 c          808 g         1206 t
ORIGIN      
        1 ctagtagtct gacaaattaa attagttact tttatatatt agctccttct taataattga
       61 taaaatgaaa accccccaga taaagctaaa aacaaaagaa tgtaagttct tacaggagtt
      121 tgtcaagaaa ggaacaaaga aagcaagggc aattgcaaga gcaaatgttt tgctcttact
      181 ggatgaagga tgggaaatca acaccatctc aaaaatgcta agggtccata ggcagagaat
      241 ttggagaata aagaagaggt tcttaaagga gggcatgaat tctgcgctgg aagaaaagcc
      301 aagaagcggc cagcccgtaa aatacacacc aatacatgaa actgagataa tagcgcaggc
      361 atgcacaaaa gccccatctg ggatgaaaag atggaccgta aggctgcttg caaaagagct
      421 taagaaaaag aaaggttttg gcacgataaa cagagagagc atccggctta ttttaaaaaa
      481 acaaaaacta agccttggct gaaaaagatg tggtgcattg gcaagcttac tccagaatac
      541 ataacacgta tgtacgatat tctggactta tacgcagagc cgtataatcc agaacagcca
      601 gtcattgggg tggatgaaaa gccaaaacag ctcatttcgg acagcagaaa gccgatacct
      661 atgaagccgg gaaagctgga aaggtatgat tatgaatacg agcgcaaagg caaggcaaat
      721 atttttatgg ctgtggagcc aaaaggcgga agaagatttc caaaggtaac gtggcagagg
      781 acaaaacagg attttgctct atacatgaaa gagcttctta tgttctatcc gtatgctgaa
      841 aaattgagga ttgtgtgtga caacctcaac acccactttg agggatcgtt ctatgagaca
      901 tttgacaaaa atgaagcaga cagaatattg agcaggcttg agttccatta taccccgaaa
      961 catgcaagct ggcttgatgt tgcagaaata gagattggca ttatggatgc tcagtgcact
     1021 ggtaggagaa taggggaaat aacagcccta aaaagagagg ttttatcctg ggcaaaaaga
     1081 agaaataagg aaagacaaca aattgattgg agatttacaa agcagatggc tgatgagaaa
     1141 ctgtctaagc attatgtaat ctaactaagt tgtcaagcta ctagcctaac ggacactgca
     1201 actgatctaa atcttcaagt aggatataaa gaatggatag atctttattc tgaactgtta
     1261 gaaagatttt gccctcagca taaggtctat gccctggaag agatggccaa gataactgac
     1321 cgccaggtta tagtcagcaa aggaacccat aaacctacta tacggcgacc gcttgctaat
     1381 attgaacgtg cagcaatcta tgcatatact tgtttaaacc tccttgtctc tcaaagaaca
     1441 agagagatag gaatggatga agaacacatc aaggtgacaa aaaccgatat ctcatatcat
     1501 tcagaagaag cctaccgtca agcaaagttt ctatttaaaa tgaaggtgca ataaattatt
     1561 ttttatatac tgtttttggg tcaaacactt tcttatctac cacctttcct ttaatatccc
     1621 tgaaaaaaca gctttcatag ccttcatgac atgcggcttt tcctatctgc tcagctttta
     1681 tcagcagggt atcattgtca caatcaacaa atatctcttt taccttctgc acattgcctg
     1741 aggtctctcc tttcatccag agcttgtttc ttgtgcggct gtaataccag acatttcctg
     1801 tctccaaagt tttcttgtac gactcttcgt tcataaatcc taccatcaac accttattgg
     1861 ttttatagtc ttggattatt gttggaacaa gcccacttag tttgctaaag tctagtttca
     1921 tgctgatttg gattatcagg aggtatattt aaatttaacc gcaatagaaa gggtcattct
     1981 tttctttttc ttaataggta cccttcagta tcattgatgt attctatggg attatctatc
     2041 actttcttat actctaccac tgccagccca taagctacga gtgccattcc ccaggcaatg
     2101 taacttatca catattctcc actagctttc ttgatgaatt gctctgcttc gggacttgat
     2161 ataaaagttg aaagctttaa cagggtcttt gctgaatctc ttgctgcaaa tcctgcatga
     2221 gtgagaaagc ctccaacagc agcgcttaat gccattatgg ttgtatctat tgtgtgacgc
     2281 ctatggaaaa atttaaggtt ggatgaatca cgaagttgtt tgtaagtcat ccagactggt
     2341 atatttttta cttttaatgc ttttggaatc acttaattgt aaaaaaatta tttaggttcg
     2401 ccctttatcc tgataggtat atctcccatt ataatatttg gagtgatgcc cttttctgtc
     2461 aagactatct tagtattgtt ttcaagagaa gcctgggtaa cttcaagttt ttttagcagc
     2521 tgggcgtttc cgacaaatga cttacttatc atctcaaatg cttttgcttc tccttctgcc
     2581 ttgagaatgg tcgcctgctt aatcccttct gcttccttga tgcttgctct tctttctcca
     2641 tctgcctgca tctcccttgc ttctgccaag tcttttgaag caattttctc gttttcagcc
     2701 ttgaccactt tgttcatggt atcctggaca tcttttggag gatctatctc ttttagctct
     2761 gtcctcacca cttctatgcc ccagctcttt gtctcttcct taagtgtttt catcagctca
     2821 tcatttatcc tatccctttc gctatttgct gacttcaggg tcaaagtgcc tattatattt
     2881 cttaatgttg tccttgcaag gcttactatc tgcacctgat attcatttac attatactgg
     2941 gatgctttta ttgattcctc gtcagatttt accctgaaat agacctgagc atcaactctt
     3001 gcattaaggt tatcgtttgt gattatctcc tgaggctctg cgttgaccat gttttcggtc
     3061 gtattaactt ttatcatcct gtcaatcact ggaattaccc aatgaaaacc agggcctgcg
     3121 aatttctcat atttacctag cctttcaact agagctcttt ctgtcggcct taccaccctg
     3181 atgccgataa agaaaataaa taatgccact atccctatta catagtatac agctgccatt
     3241 tttgatcttc ctcctgatta tgtagagatg gatatgatat tacatgtttt taaaattatt
     3301 cattttagaa aggctaataa attattttgc ctttatgcca ctgatgaaaa ttgcccagat
     3361 ctccccattg catgaaagcg tccctccaat gctatatggg ggcacagaaa gggatgtcca
     3421 ctatctcaca gaagagcttg tgaagatggg ccatgaggtg actctttatg caagcggcga
     3481 ctcaaagaca agcgctgagc ttaaaccgat gtgcaagaaa tcgctgagga tggataagaa
     3541 ctgtgtcgat cctgtgcctg atcacctata tatggctgag agagtatgcc aggactcggg
     3601 tgattttgat ataatccatt ctcatcttga atatttagga tttcctatgt taagaagagc
     3661 aaaagcgcca agtgtcacaa cactgcatgg aaggcttgac tatccaaact atgcgggcct
     3721 atttatggag tttagggata tgcccctgat ctcgatatcc aattctcaaa gaggcccatt
     3781 accttgggct aactggatag atacggtata tcatggcatt ccaaaagaca tatatgaatt
     3841 gaatgaagga aaaggagaat atcttgcatt tcttgggcgg atatcgcctg aaaagggagt
     3901 tgacaaagcc atcaagatag cagaaagggc aaatacacct cttaggatcg cagccaaagt
     3961 aggaaaagag gatagagagt attttgaaga ggtgatcatg cctttgatcg atgaaagcaa
     4021 cgctgagttt atcggcgag
//
LOCUS       DASRUK010000146         2980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1952182, whole genome shotgun sequence.
ACCESSION   DASRUK010000146 DASRUK010000000
VERSION     DASRUK010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1952182"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..775
                     /locus_tag="VFF28_06395"
     CDS             179..775
                     /locus_tag="VFF28_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45284.1"
                     /translation="MKKGAIELSLNFIIIMIISIAVFAFGVYFLKQIMSHAENTVLVW
                     DKRTESMIEDMLDNGGKIAIPFNSKTAPRGEMTTYGIGILNMLDENIENNFALLFRYN
                     KYVNNDDPDQGLPAEFDPNTWPAFSEQALPFENGNGWKKLIKPIQNNKQEKFLMGVKV
                     PKDAPSGTYIYDLNITYMDGAAYIAYDNHIWKIYVNVP"
     gene            complement(772..933)
                     /locus_tag="VFF28_06400"
     CDS             complement(772..933)
                     /locus_tag="VFF28_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45285.1"
                     /translation="MLEKKTDPEQVKSRKWIFKLVLVLIIIAIIYYLYTHPDIIRSPV
                     NKFFEGLNA"
     gene            complement(927..1658)
                     /gene="tpiA"
                     /locus_tag="VFF28_06405"
     CDS             complement(927..1658)
                     /gene="tpiA"
                     /locus_tag="VFF28_06405"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HZX45286.1"
                     /translation="MRKVIIAGNWKMNKGIDESVALAKELKKNLAGSDKEIVICPPFT
                     SLCCVKDALKGSKIKLGAQNMYCENDGAFTGEISPMMLKDAGVKYVILGHSERRHILG
                     EDDALINKKIKCALANQLVPIFCVGETLAEREEGRMKEVITRQVKEGLKGIKEIEGIV
                     IAYEPVWAIGTGKTATPEQAEEVHVLIKGIIKQVYGNIEVPILYGGSVKASNIKELAC
                     QKNIDGVLVGGASLDAKTFVEIAAC"
     gene            complement(1807..2118)
                     /locus_tag="VFF28_06410"
     CDS             complement(1807..2118)
                     /locus_tag="VFF28_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45287.1"
                     /translation="MVGGLNILISIAVMVLSALSLYFSIKYIGGQPGTMRVVFLGLII
                     SFAVLCAVMFIGIGAGIFIGIAALFIYKVAFKLSMPKAFAAWLLHYTLVFLAILAWAF
                     A"
     gene            complement(2103..2735)
                     /locus_tag="VFF28_06415"
     CDS             complement(2103..2735)
                     /locus_tag="VFF28_06415"
                     /inference="COORDINATES: protein motif:HMM:NF018964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1461 domain-containing protein"
                     /protein_id="HZX45288.1"
                     /translation="MKKIILGLLVLCLVVFVFTFGFKVNAFRESFYKKEFVKYGVYDK
                     FAGKDIDKINTEVIGYLKGREISLDDGFFSAAEISHLKDVRGLVRGADILFYLSLVFA
                     LACGAGLFYLDKNLLNISRALMIGGIVSAVGVIAIIAFVIFGFDSIFTYFHYLLFSQG
                     GWVFGATDNIVNLYTEGFFYDMGLRVFLSILAFANILILLSLSLPRWSGD"
     gene            complement(2732..>2980)
                     /locus_tag="VFF28_06420"
     CDS             complement(2732..>2980)
                     /locus_tag="VFF28_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45289.1"
                     /translation="AYVPSGHFGGDDSGSAVPKTGTIEPEIVEPIIIKQPSKNVVDEI
                     LKAQKQVTGREITLSEFEVEEIIIKRRIKKREIRIKSS"
BASE COUNT          903 a          696 c          541 g          840 t
ORIGIN      
        1 ggcaaagtag tgatagatct ttcagggtca gatgaacact ccatgacgat ggattacatt
       61 gacaatgctc cagaagtggc agcccaaatc caggcgctta tacaaaagca caaggcaaac
      121 tattatgccc agtatgccca ggattatagg tatcagggca ttgtggacag gtattaagat
      181 gaaaaaggga gctattgaac tttcacttaa ctttataata atcatgataa tatctatagc
      241 tgtctttgca ttcggggttt attttctgaa gcagataatg tcccacgctg aaaacacagt
      301 ccttgtctgg gacaaaagga cagagagcat gatagaggat atgctcgaca acggaggaaa
      361 gatagccatc cccttcaaca gcaagacagc accaagaggc gagatgacga catatggcat
      421 aggcatattg aacatgctag atgaaaatat tgaaaacaat tttgcattgc ttttcaggta
      481 taataagtat gtaaacaacg acgatcctga ccaaggcctg ccagcagaat ttgatccaaa
      541 cacatggcct gcctttagtg agcaagcttt gccctttgaa aacggcaatg gctggaagaa
      601 acttatcaag ccaatacaaa acaacaaaca ggaaaaattc ctcatgggag ttaaagtgcc
      661 taaggatgca ccatcaggca catatatcta cgacctaaac atcacttata tggatggtgc
      721 agcatatatc gcttatgaca accatatctg gaagatctat gtcaatgttc cctaggcatt
      781 aaggccttca aagaacttgt ttaccggact tcttattata tctggatgag tgtaaagata
      841 atatattatg gcgattatta tcaagaccag caccagcttg aatatccatt ttctgctttt
      901 tacctgctca ggatctgtct tcttctctag catgctgcta tctccacaaa ggtttttgca
      961 tcaaggcttg caccgccgac aagcactcca tctatatttt tttggcacgc gagttccttg
     1021 atatttgaag cttttacaga gcctccatat aagattggaa cctcgatatt gccatatacc
     1081 tgctttatta tcccctttat cagtacatgc acttcttctg cctgctctgg agtggcggtc
     1141 tttcctgttc cgattgccca gacaggctca taagctatca ctatcccttc gatttccttt
     1201 atccctttta acccttcttt gacttgcctt gttatgactt ccttcatcct gccctcttcc
     1261 cgttcagcaa gtgtctctcc cacacagaat attggtacaa gttggttggc aagcgcgcat
     1321 ttgatctttt tgtttatcaa agcatcatct tctccaagta tatgccttct ttcagagtgg
     1381 cccagtatta catatttgac ccctgcatct tttagcatca taggagatat ctcgccggta
     1441 aaggctccat cattttcaca gtacatgttc tgcgctccaa gctttatctt gctgcctttc
     1501 agcgcatctt tgacacagca aaggcttgta aaaggggggc agatgactat ctctttatca
     1561 gaaccagcaa gatttttctt aagctctttt gcaagggcaa ctgactcgtc tattcccttg
     1621 ttcatcttcc aattgcctgc tatgatcact ttgcgcatac taataattta tgtgtaagtt
     1681 gtataaatat tttgtgcctg gattccagat gaacgagggc aagacaaacc agggcttgcc
     1741 ccagtcggtg tggttttgca gaacaagttc aagccatcct cgttgttctc accagaatct
     1801 gcttacctaa gcaaaagccc aagcaagtat cgccagaaaa acaagggtat aatgcaaaag
     1861 ccacgcagca aacgcttttg gcatagaaag cttgaaagcc accttataga taaataaagc
     1921 agctatcccg ataaagatgc ctgccccaat tccgataaac ataactgcgc aaagcacagc
     1981 aaaagatatt atcagtccaa gaaagacaac gcgcatggtc ccaggctgac ctccaatata
     2041 ctttatggaa aaataaaggg atagcgcaga caataccatc acggcaattg agataagtat
     2101 attcagtccc ccgaccatct tggcagggaa aggcttaata atattaaaat gtttgcaaac
     2161 gccagtatcg ataaaaatac cctcaaaccc atatcgtaaa aaaatccttc agtatagaga
     2221 ttcactatat tatcggtcgc tccaaacacc catcctccct gggaaaagag caggtaatga
     2281 aaataagtaa agatagagtc aaagccaaag atgacaaagg caattattgc aatcactcct
     2341 acagccgaga ctatccctcc tatcattaat gcccttgaga tattaagaag attcttgtca
     2401 aggtaaaaaa gcccagcacc gcaggcaagc gcaaaaacaa gcgacaggta aaacaggata
     2461 tctgcccctc tgacaagccc ccttacatcc ttaagatggc ttatctcagc agcagaaaaa
     2521 aatccatcat ctagtgaaat ttcccttcct ttaagatacc ctataacctc agtatttatc
     2581 ttatcgatgt ctttgcctgc aaatttgtca tatactccat actttacaaa ctctttttta
     2641 taaaagctct ctctgaatgc attcacttta aatccgaatg taaagacaaa gaccacaagg
     2701 caaagaacaa gtaaacctag gattatcttc ttcatgacga ctttattcta atctctctct
     2761 ttttaatcct tctctttatg atgatttcct caacttcaaa ctcgcttaaa gttatctctc
     2821 ttccagtcac ctgtttttga gccttaagta tctcatcaac aacatttttg cttggctgct
     2881 tgattatgat gggctcgaca atctcaggct caatagtacc tgtttttgga actgcagagc
     2941 cgctgtcatc gccgccaaag tgtcctgagg ggacataagc
//
LOCUS       DASRUK010000147         4386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_460654, whole genome shotgun sequence.
ACCESSION   DASRUK010000147 DASRUK010000000
VERSION     DASRUK010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460654"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..1918
                     /gene="glgP"
                     /locus_tag="VFF28_06425"
     CDS             182..1918
                     /gene="glgP"
                     /locus_tag="VFF28_06425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HZX45290.1"
                     /translation="MKPIAAYFSAEFGLEQGAGVPVYSGGLGILSGDFLKGCANLGLP
                     IIGVGLYYNKGIPHQKLDASGWQTEYYRPVIPTGALKTLDEKVSVPIAGGSLEVTAFE
                     VPVYSSVPGSKHYVPLVLLKEDNQVGYLYPGQDKENRLRQEMILGVGGFEMLKALGHP
                     IQIYHANEGHSALMLLPLIEKYGSLDKAREHMVFTTHTLVPAGIDAFSKETAQKVMHE
                     HMLKQAYRLSGYEGVHMMRIGMNAGISHGVSKPHQVASQYIFRDFPNIEQLTYKDNGI
                     DIPTWASPEAQRLYNCIAPGWITNPQSLEKAVLEAPQDSIMQSRLTPRWRLGQYLDND
                     PHVIRNTGYDPKRLMIGFARRAAPYKQATLVLQNVGKLGAYKDKIGIVFAGKAHPADN
                     EGKKVIQEIIHYLKEREIPMWFVEDYNMASARTLTQGVDLWLNTPRILEEAAGTSGMK
                     AAANGVPQFSTIDGWWYPQNPPEGYILPKGLVPGLTGWGLGRTPTSEDLKLLLGTEPA
                     LAQRKADKEKDSVELVEKLKYEILPMFEPDKEWMQKRRWPTVVQGAMTNASYFNTIRL
                     AQETFRDIYRIN"
     gene            complement(1915..2247)
                     /locus_tag="VFF28_06430"
     CDS             complement(1915..2247)
                     /locus_tag="VFF28_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45291.1"
                     /translation="MKTYPYHYLGPIDKLLTVREVYTAIGEVLGSRGNRRKVHPVDKD
                     EGVCLYISEGLRNKMEYEVRFNPGEQVGRPLDEICLSTFLVCGQPINAKVAREVEELL
                     RNRRPSKV"
     gene            complement(2283..3770)
                     /locus_tag="VFF28_06435"
     CDS             complement(2283..3770)
                     /locus_tag="VFF28_06435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HZX45292.1"
                     /translation="MYDVIFISGEAYFDSPLSGVAMLKRVLEKKGYKLLVVKQPKEKD
                     IEKLQKPRLFFAVSSGSIDSMVRNYTPLKKSRIDDKNLNYNESVPDRACIVYSNWIRK
                     HFHDSPIVLGGTESSLRRFVHYDYWENRLRKPILFDSRADILAYGMAEKQILEIAERI
                     RSKQDIQGVKGTCVISKEVPEGFILLPNYDEVMNSKERFCDMQNLFSNSKDLAQKIDN
                     RYVLQYKSPVYTSKDLDEYYEMPFKRDSTLPHLHGFEFSVVTHRGCIGDCSFCSLSLT
                     AGNKIVSRSEGSIMRELEYISKLPNFKGIIDDFGGPSANMYGMDCHKCENKCLGCRNL
                     DRSNKRLVALLRKARKVPGIKKIMVRSGVRFDLATDEYLDELKNHTSGKLKIAPEHVN
                     KRVLQLMNKDHGDLKTFMKRCKDRGIRLSYYLMLAHPGCGMPEAKELRNAKLDTESVQ
                     VFTPTPMTVSTCMYYTRLDPKTKKKVYVPYSYKEKKEQKRLFFGK"
     gene            3873..4274
                     /locus_tag="VFF28_06440"
     CDS             3873..4274
                     /locus_tag="VFF28_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45293.1"
                     /translation="MEISCLEGERLESFVKAYEPPQEAILFYKKWKEKSISNLFVAKE
                     GDDICALLSYSDSTPSCIKTFVEFVRENAGTLYTSTKKHPVSIYYLLASEPFTDYTFG
                     TRIIYVDALESFRRSSGTALMRKSETLQPNG"
BASE COUNT         1276 a          969 c          916 g         1225 t
ORIGIN      
        1 gaaatagttg gcattttggg tgattttata gagaaatatc gggacaagag cctgcctatg
       61 cgctggtaaa atcccaactg tcaaactgag ttagtgttaa aattagatag aaaactgttg
      121 aagtggcgag gtcaggttat agactcgaca agccatttct agctaaaata agaggtgagt
      181 aatgaagcca attgcagctt attttagcgc cgaatttggt cttgagcaag gagcgggggt
      241 accagtctat agtggaggcc tgggtatcct atcaggtgat ttcttgaaag gttgcgcgaa
      301 tctgggcttg ccaattatcg gcgtcggatt atactacaac aagggtatcc ctcaccaaaa
      361 gcttgatgca agcggctggc aaacagaata ttataggccg gtcatcccta caggggctct
      421 aaagacgctg gatgaaaagg taagtgtccc aattgccggg ggatcgctag aggtgacagc
      481 atttgaagtg cccgtatatt caagtgttcc aggcagcaaa cactatgttc cacttgttct
      541 actaaaagaa gacaatcaag ttggttatct ttatccaggt caggacaaag agaaccggct
      601 aaggcaggag atgatcctag gagttggagg ctttgaaatg ctaaaagccc ttggtcaccc
      661 tatccaaatt tatcatgcaa acgaaggcca ttcggctttg atgcttcttc cgttgataga
      721 gaagtatggc tctcttgata aagcaagaga gcatatggta tttaccacac acaccttggt
      781 ccctgctggg attgatgcgt tctcaaagga gacagcgcaa aaagttatgc atgagcacat
      841 gctaaaacaa gcatatagat tgtcaggcta tgaaggagtg catatgatga ggatagggat
      901 gaacgcagga atttctcatg gtgtatcaaa acctcatcaa gtagcatcac aatatatatt
      961 cagggatttt ccaaatattg aacaattgac gtacaaggac aacggcatcg atatacctac
     1021 atgggcatct cctgaggctc aaaggctata caactgcatt gcgccaggtt ggataacaaa
     1081 tcctcagtca cttgagaagg ctgtactgga agctccccaa gattcaatca tgcaatccag
     1141 gctaaccccc aggtggcggc ttggacaata cttagacaac gaccctcatg tcattagaaa
     1201 tactggctat gacccaaaaa ggttgatgat aggctttgca cgtagagctg caccttataa
     1261 acaggcaacc ttggtattgc agaatgtagg caaactagga gcatacaaag ataagatagg
     1321 cattgtcttt gctggaaaag cccatcctgc agacaatgaa ggaaagaagg tcatccagga
     1381 gataatccac tatctaaaag aaagggaaat acctatgtgg tttgtggagg attataacat
     1441 ggcctctgcc aggacgctta cccaaggggt ggacctttgg cttaacacgc cacgtattct
     1501 tgaagaagca gcaggcacct caggcatgaa agcagctgct aatggtgtac ctcaattttc
     1561 aactatagac ggctggtggt acccccaaaa tcccccagaa ggatatattc tgccaaaagg
     1621 tcttgttcca gggctcacag gatggggtct tggccgtact ccaactagtg aagatctaaa
     1681 attgctccta ggcacagagc ctgcccttgc tcaacgtaaa gcagacaaag aaaaggattc
     1741 ggtagagctg gttgaaaaat taaaatatga gatcctccct atgttcgagc cagacaagga
     1801 atggatgcag aaaagaagat ggcctacagt tgtccaggga gctatgacaa acgcatccta
     1861 ttttaatacg ataagacttg cccaggagac gtttagagat atatacagaa taaactagac
     1921 tttacttggc cttctgtttc taagcagctc ttcaacttca cgagcaactt tggcgttgat
     1981 tggttgtccg cagaccaaga aagtgcttaa gcaaatttca tcaagtggcc ttcccacttg
     2041 ctctccaggg ttaaatctga cttcatactc catcttatta cgcaagcctt cactgatata
     2101 caagcagact ccttcatcct tatctacagg atggacttta cgccgattac ctcttgaacc
     2161 aagcacctct ccaatggcag tataaacttc tcttacagta agaagtttat caatgggtcc
     2221 aagataatgg tatggatagg tcttcatgtc ttaaaggaaa aagggcctga ttataaagat
     2281 gcctattttc caaaaaatag tctcttctgt tccttcttct ccttatagct atacggaaca
     2341 taaacctttt tcttcgtctt gggatcaagc cttgtgtaat acatgcaggt agaaacagtc
     2401 atcggcgtcg gagtaaacac ctgcactgac tcagtatcaa gctttgcatt cctgagctct
     2461 tttgcctcag gcatcccgca gccaggatga gcaagcatca aataataaga caatcttatc
     2521 cctctatcct tgcatctctt cataaacgtt ttaaggtctc catggtcttt gttcataagt
     2581 tggagcactc tcttgttcac atgctcaggc gctatcttta atttaccaga agtgtggttt
     2641 tttaactcat caagatactc atctgtagca agatcaaacc taacaccgct tctcaccatt
     2701 atctttttta tcccaggaac cttccttgcc tttctaagca gggcgacaag ccttttattt
     2761 gacctgtcaa gatttctgca tcccaggcat ttattctcac atttgtggca gtccatgcca
     2821 tacatattgg cagaaggccc tccaaaatca tcgatgatac ctttaaaatt aggcagcttt
     2881 gaaatatact caagctccct cattatcgac ccttcgcttc ttgacacgat cttgtttcca
     2941 gcagtgaggc taagagaaca aaagctgcag tcacctatgc atccgcgatg cgtaacaaca
     3001 gagaactcaa acccgtgcag atgaggtaat gtggaatctc tcttgaaagg catttcataa
     3061 tactcgtcca aatccttgct tgtatatact gggcttttgt actgaagaac atatctgttg
     3121 tctattttct gcgcaagatc ctttgaattg ctaaacaagt tctgcatatc acaaaatctt
     3181 tctttggaat tcatgacctc atcatagttt ggaagaagaa taaaaccctc aggcacttcc
     3241 ttacttatca cgcaagtgcc tttaacacct tgaatgtcct gcttgcttct aatcctctca
     3301 gctatctcca gtatctgttt ttcagccata ccatatgcaa gtatatctgc tctggaatca
     3361 aacaatatcg gctttctaag cctgttctcc cagtagtcat agtggacaaa tcttctaagg
     3421 ctgctttcag tccctccaag gactataggt gaatcatgaa aatgcttcct tatccagttt
     3481 gaatagacta tgcaggccct atctggaaca gattcattgt agttcagatt tttatcgtca
     3541 atccttgatt ttttcagggg ggtatagttt cttaccatag aatctatgct tccactactg
     3601 acagcaaaaa ataacctggg cttttgcagc ttctcgatat ctttctcttt tggctgcttg
     3661 acaaccaata atttataccc ctttttctca agaacacgtt ttagcatggc aactccgctt
     3721 agcgggctgt caaagtaggc ttcccctgag atgaatatta catcatacat aaaaggggat
     3781 taattaggat cagttttaaa agttatggtt gctgccagca ggcgagtgct tcataggctt
     3841 gccctggaaa tgccaaatag aagtaagggc agatggaaat ctcttgcctt gaaggtgaaa
     3901 gacttgaaag ttttgtaaaa gcatacgagc caccgcagga agccatcctg ttctacaaaa
     3961 aatggaaaga aaaaagtatt tccaatctgt ttgtcgcaaa agaaggggat gatatttgcg
     4021 cattgctgtc ttactcagat tccactcctt cctgcataaa aacatttgtt gaattcgtgc
     4081 gggaaaatgc aggtacactc tatacctcaa caaagaaaca tccggtttca atatactatc
     4141 tgcttgcctc tgagcctttt accgattaca cttttggcac caggattatt tatgtagacg
     4201 ctcttgaatc tttccggcgc tcaagtggta ctgccctgat gagaaaaagt gaaactctcc
     4261 agcctaacgg ctgaagcctc tttctaataa tctattgcat tgatgccctt ggggcataga
     4321 aggggataaa ccccttggat tctgagtaac ataaatcttt ccccttttga gatggtgagc
     4381 cttcgg
//
LOCUS       DASRUK010000148         3442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_460687, whole genome shotgun sequence.
ACCESSION   DASRUK010000148 DASRUK010000000
VERSION     DASRUK010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460687"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(313..399)
                     /locus_tag="VFF28_06445"
     tRNA            complement(join(313..347,363..399))
                     /locus_tag="VFF28_06445"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Gly,seq:gcc)
     gene            complement(405..518)
                     /locus_tag="VFF28_06450"
     tRNA            complement(join(405..440,482..518))
                     /locus_tag="VFF28_06450"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(483..485),aa:Val,seq:tac)
     gene            588..1340
                     /locus_tag="VFF28_06455"
     CDS             588..1340
                     /locus_tag="VFF28_06455"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HZX45294.1"
                     /translation="MEEKAPHEQIFDILFDKDEVTWQSIIQELVKSEQMDPWDINVSL
                     LTQKYIDMIKMLKEHDFRVSGKVLLAAALLLKIKSNRLVGEDIEYLDRLISQEDEQEL
                     LFEDDLQPRPVENIPKNLIPRTPQPRKRKVSIYDLMNALERALEVKRRRVLKSIPPLN
                     VEIPKKSRDISEVIKEIYGKIKAWFWDNKDEKLTFSKLTPSGSKQDKISTFIPLLHLT
                     NQRKIDVFQQQHFGDIDISLRKEEMEKEFKSL"
     gene            complement(1531..1728)
                     /locus_tag="VFF28_06460"
     CDS             complement(1531..1728)
                     /locus_tag="VFF28_06460"
                     /inference="COORDINATES: protein motif:HMM:NF002242.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17e"
                     /protein_id="HZX45295.1"
                     /translation="MGRIKTAQIKRVTRQLINNNAAQFSTDFKSNQAVVGKLISTPSK
                     RLRNKVTGYVTKMTKQRKENA"
     gene            complement(1731..1946)
                     /locus_tag="VFF28_06465"
     CDS             complement(1731..1946)
                     /locus_tag="VFF28_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45296.1"
                     /translation="MYYYIKSRKKGTCEWKALRQDGKIIVLNNFEKAKEKIASMADHA
                     CKIVTMKKHPSDKESRIKGKSFLKEDW"
     gene            complement(1975..2133)
                     /locus_tag="VFF28_06470"
     CDS             complement(1975..2133)
                     /locus_tag="VFF28_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45297.1"
                     /translation="MPRGRKKKEETEDVEETTDEDEPVEEDTSDIDKDDYDDEDEDNT
                     EEEKEDEY"
     gene            complement(2256..3137)
                     /locus_tag="VFF28_06475"
     CDS             complement(2256..3137)
                     /locus_tag="VFF28_06475"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZX45298.1"
                     /translation="MTLLCLKQNAGTGFEAFIEEELIEMPHVVAYLGLETIPHPDTLN
                     KFENSIKQTTLQLVLLQTTARTGIKKLFLGQDGTGFKAKRASSYYHFRIEYCQRTNKK
                     RKPGRPRKKKKRRRYLYVQIMVELRTQMPLGVSISRKPSGDAKKFIPVAKKVMKLGKS
                     VKIVCLDRGYDDEKVHEYIREIMDALSIIPARNKDVPIWRTKGKYRKQMKRGYSRKYY
                     FMRNKNETVNSVVKRKWGDSTLAMTWRNQNKEVLFRLIFYAARRFISIFLFCISIKII
                     IPLKMRASYCFSEGGFL"
BASE COUNT          935 a          774 c          640 g         1093 t
ORIGIN      
        1 catattctct tacggagaaa tttttgaacc ccagaccgcc ggctgtctta tgccggatgt
       61 ttagaacgtg gccatataag accggcgctc taaccaggct gagctatggg cccatgaaat
      121 ctttagagaa tttaagcggt ttataaagat ttttgtttgg tccaacacag agcaaacatg
      181 tattaaccag caaagttgtt tatcactaag atataataaa caaatttaaa atggcggttt
      241 cctgatttat ggaaagcaaa ttaagcattg atgactacgc atttgcgtta tagaactcaa
      301 gcagcataaa aatgcgccag ccgggattcg aacccgggcc acagcctctt tgcaagatta
      361 tttggcaagg ccgtatcgta accacttgac tactagcgcg aaggtgggcc cagccagatt
      421 cgaactggcg acctacacct atcatatgat gggcaattgt gatcaacttg attgcctacc
      481 ttgtaagggt gttgtcatag ccactagacc atgagcccaa atttagatag gagtgtttat
      541 tagccatata aaaacctttt tatatttgcc atattctatt gaatgcaatg gaagaaaaag
      601 ctccccacga gcagattttc gatattcttt tcgacaagga tgaggtgaca tggcagtcta
      661 tcatccagga gcttgtcaag tccgagcaga tggacccctg ggatatcaac gtctccttat
      721 taacccagaa atacatcgac atgataaaaa tgctcaaaga gcatgatttt agggtctcag
      781 gcaaagtcct tcttgcagca gctttgctat taaagatcaa atccaaccgc ttagtaggcg
      841 aagatatcga atacttagac aggcttatct cgcaggaaga cgagcaggaa ctgttatttg
      901 aagacgatct ccagccaagg ccggttgaaa acatcccaaa aaaccttatt ccaaggactc
      961 cgcagccaag gaagcgaaaa gtctctatat atgacctgat gaacgctctg gagcgggctt
     1021 tagaggttaa aagaagaagg gtcttaaaaa gtatccctcc attaaacgtt gagattccaa
     1081 aaaagagcag agacatctca gaggttatca aggagatcta cggcaagata aaagcatggt
     1141 tctgggacaa taaagacgaa aagctcactt tctctaagct tacgccttca ggatcaaaac
     1201 aagacaagat atccactttc atcccccttc tccatcttac caatcaaaga aagatagatg
     1261 tcttccagca gcagcatttt ggtgatatag atatctcgct tagaaaggaa gagatggaaa
     1321 aagagttcaa gtccctttag ccataaattc tatggattgt cccttcagaa aaaagcagat
     1381 gacgtcatgc aggcaaaaat gcctagcaag ccagaataca agggcggcat atgaaaccca
     1441 gaactggcgg caaaaagctc cctatcattc acgatgataa caccaagcat catcaagaag
     1501 cagacaggca tcactgctgc ttatgtgttt ctaagcgttc tcttttcgct gcttggtcat
     1561 cttggtgaca tagccagtga ccttgttcct tagccgttta gatggcgtag atataagttt
     1621 tcccacaact gcttggtttg acttaaaatc agtggaaaat tgtgctgcat tattgttgat
     1681 aagttgtcgt gttactcttt ttatctgtgc tgtttttatt cttcccatga ttaccagtct
     1741 tccttaagga atgattttcc ttttattctg ctttctttgt cagaaggatg ctttttcatt
     1801 gtgactatct tgcaggcatg atcagccatg ctggctattt tctcctttgc cttctcaaaa
     1861 ttgttaagta cgatgatctt tccatcctgc cttagtgcct tccactcaca ggtccctttc
     1921 ttcctagatt ttatgtaata atacatatca ttcccctcct aaataaaaaa caaattaata
     1981 ttcgtcttct ttttcttcct ctgtgttatc ttcgtcttcg tcgtcatagt catctttgtc
     2041 tatatccgat gtatcttctt ctaccggctc gtcttcatct gtcgtctctt caacatcttc
     2101 tgtctcttct tttttcttcc ttcctcttgg cacagacatc acctcagttt cgatataacc
     2161 gaaagcatgg ctttatgtat accccattaa tattacctta ttgccggaag atttagcatc
     2221 attgtcttat atttaaagct ttctacactt gggctctaca gaaaccctcc ctctgaaaaa
     2281 caataacttg ccctcatttt cagaggaata attattttta ttgatataca aaacaaaaaa
     2341 atagagataa atctacgtgc tgcatagaag attagcctaa aaagcacctc tttgttctga
     2401 ttcctccatg tcatggcaag ggttgagtct ccccactttc gctttacaac agagtttacc
     2461 gtttcgttct tgttcctcat aaaataatat ttcctgctgt agcctctctt catctgcttc
     2521 ctgtattttc cttttgttct ccagataggc acatccttgt ttcttgcagg aatgattgag
     2581 agggcatcca taatctccct tatgtactcg tggacttttt catcatcata tcccctgtca
     2641 agacagacta tcttaacaga tttgcctagc ttcatgacct tttttgctac agggatgaat
     2701 ttttttgcat cccctgaagg ttttctgctt attgacacgc ccaagggcat ctgggtcctt
     2761 agttcaacca ttatctgaac atatagatat ctcctgcgct tcttcttttt ccttggcctg
     2821 cccggctttc tctttttgtt tgttctttgg caatactcta tacggaaatg atagtaggag
     2881 cttgcccttt ttgccttaaa tccggttcca tcctgcccta agaataactt ctttatgcct
     2941 gttcttgccg ttgtctgtag caataccaac tgaagggtgg tttgctttat gctgttctca
     3001 aatttattga gtgtgtcagg atgaggtatt gtttcaagcc caagatatgc taccacatgg
     3061 ggcatttcaa tcaattcttc ctcgataaat gcctcaaatc cagtccctgc attctgcttt
     3121 aggcagagca gggtcatttt ttggtaatta tcatatatct tgttgctata tttagaagaa
     3181 tattttttta tacctgctct tttacaaagc tctttgatac gaccgcaaat ctgtatcagg
     3241 ggggtttcct tgaacttcaa ggtcatggtt ggttcctcca accaccccca ttttactaaa
     3301 gaaagtgaaa ctctccagcc taacggctga agcctctttc taataatcta ttgcattgat
     3361 gcccttgggg catagaaggg gataaacccc ttggattctg agtaacataa atctttcccc
     3421 ttttgagatg gtgagccttc gg
//
LOCUS       DASRUK010000149         4626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_779318, whole genome shotgun sequence.
ACCESSION   DASRUK010000149 DASRUK010000000
VERSION     DASRUK010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779318"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..542
                     /locus_tag="VFF28_06480"
     CDS             150..542
                     /locus_tag="VFF28_06480"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HZX45299.1"
                     /translation="MSKLKTELELLEKDTFYSGAIAHAYYAIFYATKALLLKERVKTK
                     SPNIHKATLDSFAYYFVISGKLDLELLKIYRSAIIRADSLLGLLLSEKDKRGEFTYQK
                     LPDANKQPAEESIKNAIIFLTHINKLIN"
     gene            590..1228
                     /locus_tag="VFF28_06485"
     CDS             590..1228
                     /locus_tag="VFF28_06485"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZX45300.1"
                     /translation="MRYDLHIHTYNSACSTLRPETILKAAKRVGLDGIAVGDHNTVKG
                     GQEVKKLNKDKDFEVIPSIEVSTDRGHVLGLYIREEIKSREFFSVTDEIKKQGGIVIL
                     AHPFRVLPHLRAKIKGVDLKKCLDGIECFNARTSYFSNRSAVKTADKFNLAKTGGSDS
                     HFSFEIGRSYTVFEGDLRDAVRKRKTQACGSNVTGLMGSSFSFFKKHIFRVW"
     gene            1228..1743
                     /locus_tag="VFF28_06490"
     CDS             1228..1743
                     /locus_tag="VFF28_06490"
                     /inference="COORDINATES: protein motif:HMM:NF013981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HZX45301.1"
                     /translation="MILQALYFMLPAYIANMAPVIARGWIKPLCKPVDGGKGIFGNNK
                     TYNGFFFGVLFALGVTFIQSILYNMVFFREISLVNYNYWFIVGFLLGFGALFGDLIKS
                     FFKRRIGIAPGSRFFPWDQLDFVFGALIFSSLIVKLTFSMMLVIAIVSVLGHIAVNHT
                     AYYLRIRKEKW"
     gene            1737..2342
                     /locus_tag="VFF28_06495"
     CDS             1737..2342
                     /locus_tag="VFF28_06495"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HZX45302.1"
                     /translation="MVIIFAALIIIVLGFYKFVFLRDPKREIPSGNNLLSPADGKIIN
                     ILKLSKKEIRIAKGSGMIRALVPESCKDGWLITIMMTVFDVHVQRAPIAGEVTDIKYS
                     KGKFKNAVYGHALENGLENEKNEITIKSEIGDVKVIQIAGYIARRIQCFVSKKQKVNK
                     GERIGRIVIGSQVCLIVPRKVNLAVKNGERVVAGKTIIGTI"
     gene            2339..3403
                     /gene="pssA"
                     /locus_tag="VFF28_06500"
     CDS             2339..3403
                     /gene="pssA"
                     /locus_tag="VFF28_06500"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HZX45303.1"
                     /translation="MIAKKRFKQLFVKKKRGLTLVFLRILLAAIIFSVVMSSKDELAL
                     YLFGLAAFAAFIDGFLAKKNRIESQLRTIFDSLADKLLVNLIAIALFLKGALPLWVMT
                     IYLAKDAVIIIGALMLLLRNTKTIFSPNAIDKISLFAQVFTLFIILLGYLDYVLVWLS
                     VILVIASLISTAFKSGIRVVTYKTDLEEIRFTKLIRLPDIFTLLNVFMGLLSIILTIN
                     EQYSYAVSAMILAVVFDVVDGKVARLVKRNGDFGKQLDSLADTISFGVAPAIFGFALI
                     KTNIAVVVFALFLFAGVLRLARYNIMEFTQDFAGMPITVNGVLIPLIFLAGVPTKVFP
                     YIYLVLAVLMVSPIRIKRLF"
     gene            3624..4001
                     /locus_tag="VFF28_06505"
     CDS             3624..4001
                     /locus_tag="VFF28_06505"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HZX45304.1"
                     /translation="MTKIDCGECSDCCKDPLNPVLMPYEENDYPGSIVNINTPYRVIH
                     TLAKKDGHCIFLIDGKCTIYNKRPFECRIYPYLLDFSRKPAGFKLDKRFCKKLHTLNA
                     EKISLDNGQFDDVWIRAYESLDC"
     gene            complement(3990..4442)
                     /locus_tag="VFF28_06510"
     CDS             complement(3990..4442)
                     /locus_tag="VFF28_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45305.1"
                     /translation="MSLLNYSLAALVAYLGLISGLMLSIVAKEELKAGRKYFFILQKV
                     LLVLIFSILLVLRPGYLPIFLSVIFMIYLLVHKNLSSPMIYLFLAGVFYLSSIRLDIF
                     IVEASLIFLYGFPTGSIVASKSIKESIMKISRYSSFVLLAIVLFLLAV"
     gene            complement(4439..>4626)
                     /locus_tag="VFF28_06515"
     CDS             complement(4439..>4626)
                     /locus_tag="VFF28_06515"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HZX45306.1"
                     /translation="RAALCYDEETAKLAREHNNANILCLGSRTEPSKFFKKIIRAFLA
                     AKFSDEERHHKRVQQIE"
BASE COUNT         1411 a          859 c         1003 g         1353 t
ORIGIN      
        1 gtctcttaaa aatacttgac gaatgatgaa atttggttct taatgccatc tatatcacct
       61 cttgctaatt acagaagggg tacttatata agaacttttc tatttaactt actataaaac
      121 aatctgaaac cctgtttaag atctctgaga tgagcaagct aaaaacagag cttgaattgc
      181 ttgagaaaga tacgttttat agcggtgcaa tcgctcatgc ttattatgca atcttctatg
      241 ccaccaaggc cttgctgttg aaagaaagag tgaagacgaa aagcccaaat atccataagg
      301 caacactaga ttcatttgct tattattttg tcatcagtgg aaagcttgac ttggaattgt
      361 taaaaatcta tagatcggct ataatccgtg ctgattcctt actgggtctt ctcttatcag
      421 agaaggataa gagaggagaa ttcacttatc agaaacttcc tgatgccaat aagcagcctg
      481 cagaagagtc gataaaaaat gctataattt ttcttacgca catcaataaa ctcataaact
      541 aacgcaatct ttttatatga accgctttat tgtacacttt ggtggtttta tgagatatga
      601 cctgcacatc catacctaca attcggcctg ctctacactt aggccagaga caatactaaa
      661 ggcagcaaag cgagtcgggc ttgacgggat tgcagtagga gaccataaca ctgtcaaggg
      721 cgggcaggaa gtaaagaagc tcaacaagga taaggatttt gaggttatcc cttctatcga
      781 ggtaagcacc gacagggggc atgttcttgg gctttacata agggaagaga taaagtcaag
      841 ggagtttttt tctgtcactg atgagatcaa aaaacaggga ggaatcgtca tccttgcgca
      901 cccttttagg gtactgcctc atcttagggc aaagatcaaa ggagttgacc taaagaaatg
      961 ccttgatgga atagagtgct ttaatgcaag gacttcatat tttagcaaca ggagcgcggt
     1021 taagactgcg gataaattca atcttgcaaa gacaggcggc tctgactcac atttttcttt
     1081 tgagataggc agaagctata ctgtgtttga aggagactta agagacgcag tcaggaagag
     1141 aaaaacacag gcatgcggct ctaatgtgac tggacttatg ggaagcagct tcagcttctt
     1201 taaaaaacac atattcaggg tgtggtgatg atactacaag cactttactt tatgctgcct
     1261 gcatatatcg ccaatatggc gccagtcatc gcaagaggat ggattaagcc actatgcaag
     1321 cctgttgatg gcggcaaggg tatatttgga aacaacaaga catacaatgg ttttttcttt
     1381 ggcgtactgt ttgcgcttgg agttacattt atccaatcta tcctttataa tatggtattt
     1441 tttagagaaa taagccttgt caactacaac tattggttta tcgtgggctt tctcttgggt
     1501 tttggagctt tgttcggtga tctcataaag agctttttta agagaaggat aggcatcgcg
     1561 cctggaagca ggttttttcc atgggatcag cttgactttg tgtttggagc gcttatcttt
     1621 tcaagcctta tagtaaaact tacatttagc atgatgcttg tcatcgcaat agtaagcgtt
     1681 cttgggcata tcgctgtcaa tcacactgca tactatcttc gaataagaaa agaaaaatgg
     1741 taatcatctt tgctgctttg atcattatag tgcttggatt ttataagttt gtttttctca
     1801 gggatccaaa aagagagata ccttccggaa ataatctcct ctctcctgct gatggcaaga
     1861 taatcaacat acttaagctt tctaagaaag agataaggat tgccaaagga agcggcatga
     1921 taagggcgct tgttcctgag agctgtaaag atggatggct tatcacgata atgatgactg
     1981 tctttgacgt gcatgtgcaa agagctccga ttgccggtga agttactgat atcaaatatt
     2041 ccaaaggaaa gttcaagaat gctgtgtatg gacatgccct tgagaacgga cttgagaatg
     2101 agaagaacga gataacaata aaaagtgaga taggagatgt aaaagtaata cagatcgcag
     2161 gctacatcgc aagaagaatc cagtgctttg taagtaaaaa gcagaaggtt aataagggag
     2221 agcggatagg taggatagtt ataggaagcc aggtatgcct gatagtccca aggaaggtca
     2281 acctggctgt gaagaacggt gagagggtgg ttgcaggcaa gaccatcata gggacaatat
     2341 gatcgcaaaa aaaaggttca aacagctgtt tgtcaaaaaa aagagaggcc ttaccctggt
     2401 tttccttcgt atacttcttg cagccatcat cttttctgtc gtcatgtctt caaaggatga
     2461 gcttgcgctt tatctgtttg gccttgcagc gtttgctgcc tttatagatg gatttcttgc
     2521 aaagaaaaat aggatagaat cccagcttag gactatattt gattccttgg cagacaagct
     2581 gctcgtcaat cttatcgcaa tcgcgttgtt cctgaagggc gcgcttcctt tgtgggtgat
     2641 gacaatatat cttgcaaagg atgcggtgat aatcattgga gcattgatgc tgcttttgag
     2701 aaatacaaag acaatctttt ctccaaatgc catagacaag ataagcctct ttgcccaagt
     2761 gttcaccttg tttataatcc tgctagggta tcttgattac gtcttggtct ggctttcagt
     2821 catacttgtc atcgcttcac ttatctcaac agcattcaag tcagggataa gggtggtcac
     2881 atataagaca gaccttgagg aaataaggtt tacaaaactc atcaggcttc ctgatatatt
     2941 tacactgctc aatgttttta tggggcttct ctccataata cttacgataa atgagcagta
     3001 ttcatatgcg gttagtgcta tgatacttgc tgttgttttt gatgtagtgg atggaaaagt
     3061 cgcaaggctt gtaaaacgaa atggagactt tggaaagcag cttgacagcc ttgcagacac
     3121 tatcagcttt ggggttgcgc ctgcgatatt tgggtttgca cttatcaaga caaacattgc
     3181 tgtcgtcgtc tttgcgctgt ttttatttgc aggagtcctt cggcttgcca ggtataatat
     3241 catggaattt acgcaggatt ttgcagggat gcctataact gtaaatggag tattgatccc
     3301 gcttatcttt ttggccggcg ttccaacaaa agtgtttcct tacatctatc tggtgcttgc
     3361 ggttttgatg gtgagcccga taaggataaa gagattgttc tagtcttctt atagccctag
     3421 ccagtttatc catagaagct tccagctaag tcttataaac ttaaaaaaaa tcttttcata
     3481 atggctcttt caagggaagt gagaaaccac ccaaatatac cctacccata tagggatatc
     3541 tctaaatggt ttaggagagg ccaatatatt tttaccaacg agtagccaga tatattttaa
     3601 tataaatgcg atttctattc ttcatgacca aaatagattg cggagaatgt agcgactgct
     3661 gcaaagaccc attaaatcct gtactcatgc catatgagga aaatgattat cccggatcta
     3721 tcgtcaacat caacacgcct tatagagtga tacatacttt ggcaaagaaa gatgggcact
     3781 gcatattcct tatcgatgga aaatgcacca tctataacaa aaggccattt gagtgcagga
     3841 tatatccgta tcttctggat tttagcagaa aacctgctgg ctttaagcta gacaaaagat
     3901 tctgcaagaa gttgcatacg ctaaatgctg agaaaataag tttagacaac ggtcagtttg
     3961 atgatgtttg gataagggct tatgaaagcc tagactgcta ataaaaataa aactatcgcc
     4021 agcaaaacaa atgaagaata tcttgagatt ttcataatcg actcttttat actttttgag
     4081 gcgacaatcg agcctgttgg aaagccgtat agaaatatca acgatgcttc aacaataaag
     4141 atgtccagcc taatagaaga gagataaaaa actcctgcca ggaaaaggta gatcatcggc
     4201 gaagatagat ttttatggac caacaggtag atcatgaaaa taactgataa aaatatcggc
     4261 agatatcctg gcctcaatac caacaggata gaaaatatca gcacaagcaa aaccttttga
     4321 aggataaaaa aatattttct tcctgccttc agctcttctt ttgcaacgat agaaagcatc
     4381 aatccagaaa taaggccaag gtaggccaca agcgctgcaa gggaataatt tagcaggctc
     4441 attctatctg ctgcactctt ttatggtggc gctcttcatc tgagaacttt gccgcaagaa
     4501 acgctcttat tatctttttg aagaactttg aaggctctgt ccttgaaccg aggcatagga
     4561 tatttgcgtt gttatgctct cttgcaagct ttgctgtctc ttcgtcataa cataaggctg
     4621 ctctta
//
LOCUS       DASRUK010000150         5163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1783499, whole genome shotgun sequence.
ACCESSION   DASRUK010000150 DASRUK010000000
VERSION     DASRUK010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783499"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VFF28_06520"
     CDS             <1..270
                     /locus_tag="VFF28_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HZX45307.1"
                     /translation="ELVQIILSFRLFLPKLSIAVSTREPAWLRDNLIKLGVTKLSAGS
                     TTAVGGHTTHQESVQFDISDRRDVEEIKHMIKSKGYQPVVKDWWT"
     gene            267..887
                     /gene="thiF"
                     /locus_tag="VFF28_06525"
     CDS             267..887
                     /gene="thiF"
                     /locus_tag="VFF28_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS adenylyltransferase
                     ThiF"
                     /protein_id="HZX45308.1"
                     /translation="MNEFDKTARCAIGNMDYPRLNRIKIGIAGAGGLGSNCALNLVRS
                     GFKKLKIVDFDKVEFSNLNRQFYFYEQVGKEKIEALKENLLKINPDLELDMVNARIDA
                     GNVACLFEDCDVIAEGLDSAEDKKMLVERMLAENKFVVSASGICGYGKCDDIKVHHLM
                     HNLAVVGDLKTSTDCRAPISPRVNIVAAKQADIILSYVMGNLDGKK"
     gene            983..2158
                     /gene="argH"
                     /locus_tag="VFF28_06530"
     CDS             983..2158
                     /gene="argH"
                     /locus_tag="VFF28_06530"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HZX45309.1"
                     /translation="MAKLWQDKKLDKKIEKFTVGDDYLLDKKLIMFDCKASIAHSKML
                     NKIGIIKDDEQKKLEKELNNIIKLSKEGKFSITLEDEDCHTAIENHLTEKLGELGKKI
                     HTCRSRNDQVMAAMKLYEKEEMQKVQALAEQLALSLKKLSKDTTPIPGYTHMQKAMPS
                     SVKMWCDGYIESLSDDMKVLANALLVIDKNPLGTGAGYGLPIAVDKEVTKKELGFASN
                     YQSITYVQISRGKHEALILNAMVNLMLTMNKMASDLIMFSMQEFGYFTLPSEFTTGSS
                     IMPQKKNPDILELVRAKYSIVLGYEFQLKTLIGNLISGYNRDLQLTKEPIMKGIDVTK
                     DCLEIMALVISGLKINKNNCQKAMTPDLFATEKSYKLVLKGKSFREAYKEVKKDLNK"
     gene            2231..3223
                     /locus_tag="VFF28_06535"
     CDS             2231..3223
                     /locus_tag="VFF28_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZX45310.1"
                     /translation="MVNFGIIGAGGKMGKAFSANVPSSFNLLAVCDTDPERLTSFPPS
                     IARYDSCEAMLKRHTLDLVAVATHNRSHFKNTVLALESGSSVLVEKPVSSDLEEALQL
                     KQIKLRHGQRIAVDYEVRFSKGARTAKRFIERYGLMPLETEMIWVKKRDNKPHDGVII
                     QDLTHALDLTAHYLFRADNVYCKDATIGIRAGKEVFADTSLYLSRWHKVHAIASYISE
                     HYERSLKIKCKDRVGKIYFIELKFDISVDGTSSTDSICIYDSEGNSYLGQQSPTGHSP
                     IIVKTKKLERQLCAVGDWIATGRQTPEICTLDQAVRNLQICYAAKGYRSQPKPL"
     gene            3248..4900
                     /gene="argS"
                     /locus_tag="VFF28_06540"
     CDS             3248..4900
                     /gene="argS"
                     /locus_tag="VFF28_06540"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HZX45311.1"
                     /translation="MDFKQEIIDALRKETKLEEIHLEVPPNPELGDFAFPCFSLAKAY
                     KKAPVQIAQDLASKIKIKSMSKIEAKGPYLNFFVNKENFTQDILTKMQKSENYGSLDL
                     GKKKKILVEHTSINPNASPHVGRARNALIGDSIVRLLKFQGYDVETHYFVNDIGKQIA
                     MLVLGAEGKKKVTFDDLLKIYVKINKQVEEKPELEKEVFQLLNRLEKGDKEIITKFRK
                     MVEVCVKGQSEILSDLDIHYDVYDYESDYLWNKKTEEILKLLEKTGKLFTDEEGRKVL
                     DQEGFDLAMKSPVLVLTRADGTSLYPLRDLAYHIDKKKDENIIVLGEDQKLYHQQIKA
                     ALSLIKFKAPRVVHYSFVLLQEGKMSTRKGTVVLLEDFMKEALDKSKKEIKQRHGEVK
                     EEDAKKIAYGAIKYAILKVSNEKNVTFDWHQALNFEGDSGPYLQYAYARISSIFEKHG
                     DKIKKDVDYSLLKEPQELELIKTLGTFPDIVKEAAEQLKPNLIANYLFHLASQFSVFY
                     NACPVLKAEEDLMQARLVLIDSVRKVLKNGLSLLGIDVLDKM"
     gene            4937..>5163
                     /locus_tag="VFF28_06545"
     CDS             4937..>5163
                     /locus_tag="VFF28_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45312.1"
                     /translation="MISECVMHVGVVLPLRSEQGPLRVCLIPQAGGYIVATYPLSNSN
                     NPLAVQEYGDLESAIKAHREVFEALEKDGRL"
BASE COUNT         1756 a          925 c         1161 g         1321 t
ORIGIN      
        1 gagctggtcc agataatctt aagctttagg ttattcttgc caaagctgag tatagctgtg
       61 tctacacgag aacccgcatg gctaagggat aatctgataa aacttggagt gactaagtta
      121 tcagcaggct ctacaactgc agtagggggc catacaactc accaggagtc tgtccagttc
      181 gatatatctg atagaaggga tgttgaagag ataaagcaca tgatcaaaag caaaggctat
      241 cagccagttg taaaggattg gtggacatga acgaatttga taagacagca aggtgcgcta
      301 tcggaaacat ggattacccc agactgaaca gaataaagat aggcatagca ggagcaggag
      361 gattaggaag caactgtgct ctcaacctgg tcaggagcgg gtttaaaaaa ctaaagatag
      421 ttgattttga caaggttgag ttctctaatc taaacaggca attttatttt tatgagcagg
      481 ttggaaaaga aaagatagag gcattgaagg aaaatctcct gaagataaat cctgatctgg
      541 agctggatat ggtcaatgcc agaattgacg caggaaacgt tgcctgccta tttgaagact
      601 gcgatgtgat agctgagggc cttgacagcg ctgaggataa aaagatgcta gttgaaagga
      661 tgcttgctga aaataagttt gtggtatctg catcaggaat ctgcggatat ggaaaatgtg
      721 atgatataaa agtgcatcat ctgatgcata accttgccgt ggtcggtgac ttaaagacaa
      781 gcactgattg cagggcccct atatctccca gggttaatat tgtggcagca aagcaggcgg
      841 atataatact gtcatatgtg atgggaaatc tagatggtaa gaaatgatga aatggcttat
      901 tagtctaaaa ttagacatta atgtttaatt gtttctttta tcggtcaata aatttataaa
      961 tagctaactc ggattccagc ttatggctaa actctggcag gataagaaac tggacaaaaa
     1021 gatagagaaa tttacagtcg gagacgatta tctattagat aaaaagctca taatgtttga
     1081 ctgcaaagca tccatcgctc actcaaagat gctcaataag ataggtatca taaaggacga
     1141 tgagcaaaag aaactggaaa aagagctaaa taatattatc aaattatcaa aagaaggcaa
     1201 attttcgata actcttgaag atgaagactg ccatactgca attgagaacc atctaacaga
     1261 aaagcttgga gaattaggca agaagataca tacctgcagg agcagaaacg accaggtgat
     1321 ggcagccatg aagctgtatg agaaagagga gatgcaaaaa gtccaggctc tcgcagagca
     1381 attggcatta tcgttaaaga agctttcaaa agatactact cctattccag gctacactca
     1441 tatgcaaaag gcgatgcctt cctctgtcaa gatgtggtgt gatggatata tcgaaagcct
     1501 ttcagatgat atgaaagtct tagcgaacgc tcttttggtt attgacaaaa accctcttgg
     1561 gaccggagca ggatatggcc ttcctatagc tgtggataag gaagtgacaa aaaaggagct
     1621 tggctttgct tcaaactacc aatcaatcac ctacgtccag atatcccgag gcaaacatga
     1681 agcgttgatc cttaacgcta tggtcaactt gatgcttacc atgaataaga tggcctctga
     1741 ccttataatg ttctctatgc aggagtttgg atactttact ctgccctctg agtttaccac
     1801 aggaagttct ataatgcctc aaaagaagaa tcctgatatt ctggagctgg ttagggcgaa
     1861 atattcaatc gtattagggt atgaatttca gctaaagact cttataggca atcttatatc
     1921 gggttataat cgtgatcttc agcttacaaa agagccgatt atgaaaggga ttgatgtcac
     1981 taaagattgc cttgaaataa tggctctggt tatttcaggc cttaagataa acaagaataa
     2041 ctgccagaag gcgatgactc ctgatctctt tgcgactgag aaatcttaca aactggttct
     2101 aaagggaaag agctttcggg aagcgtataa agaagtaaaa aaggacctga acaagtagat
     2161 ttaaaaacat tgtctcattc catcagtaaa aaagaggtag gtagcagcct ctttgggctg
     2221 gttctttgtt atggttaact ttggaataat cggggcagga ggaaaaatgg gcaaagcttt
     2281 ttcagctaat gtaccatcga gttttaacct tcttgctgtt tgtgatactg atccagaaag
     2341 gctcacttcc tttcctccta gcattgcaag atatgactct tgtgaagcta tgcttaaaag
     2401 acatacattg gatctagttg cagtggccac ccataaccgc agccacttta aaaatacagt
     2461 cttagctctt gaaagcggaa gcagcgtcct tgttgaaaaa ccggtttcct ctgacttgga
     2521 ggaagccctc caattgaagc agattaaatt acggcacggt cagaggattg cagttgacta
     2581 tgaagtaaga tttagcaaag gtgccaggac tgcaaagcga tttattgaaa gatatgggct
     2641 tatgcccctt gaaacagaga tgatctgggt taaaaaaaga gataataaac ctcacgacgg
     2701 agttatcata caagacctta cacatgcttt ggatttgaca gcccattatt tgtttagggc
     2761 agataatgtg tattgcaaag atgctacgat tggaataagg gctggcaagg aagtctttgc
     2821 cgatacttca ttgtatctat caagatggca taaggtccat gcgatagcct cctatattag
     2881 cgagcactat gaaagaagcc ttaaaataaa atgcaaggat agagttggca aaatatattt
     2941 tattgagctt aaatttgaca tttcagttga cggtacctct tccactgaca gtatctgcat
     3001 atacgactca gaaggaaata gctaccttgg gcagcaaagc ccaacagggc atagtccaat
     3061 tatagtgaaa actaaaaaat tggaaaggca attatgtgct gttggagact ggatagctac
     3121 tgggagacaa acacctgaaa tctgtacgtt agatcaggct gtaagaaacc tacaaatttg
     3181 ctatgcagcc aagggatata gatcccaacc aaaaccttta taacttttct ttgaaatttc
     3241 tctacaaatg gacttcaaac aagagataat cgacgctttg agaaaagaga caaagcttga
     3301 agagatacat cttgaggtgc cgccaaaccc tgagcttgga gactttgcat ttccatgctt
     3361 ttcattagca aaggcttata agaaagctcc tgtccagatt gcacaagacc ttgcctcaaa
     3421 gattaagata aaaagcatgt caaagataga agcaaaaggc ccttacttaa acttctttgt
     3481 caacaaagag aacttcacac aagacatact tacaaagatg caaaaaagcg agaattatgg
     3541 ctcactggat cttgggaaga aaaaaaagat attggtcgag catacctcca tcaatccaaa
     3601 cgcttctcca cacgttggaa gggcaagaaa cgctttgata ggagactcga tagtaaggct
     3661 tctaaagttc caaggctacg atgttgagac acattatttt gtcaatgata ttggaaagca
     3721 gatagctatg cttgtgctcg gcgcagaagg caaaaagaaa gttacattcg atgatctact
     3781 taagatttac gtcaagataa acaagcaagt cgaagaaaag ccagagcttg aaaaagaagt
     3841 gttccagctg ttaaatcggc tggaaaaagg tgacaaagag ataatcacca agtttagaaa
     3901 gatggttgaa gtctgcgtaa aaggccagag tgaaatcctt tcagacctgg atatacacta
     3961 tgatgtatac gattatgaat ctgactatct ttggaataaa aagacagaag aaatattaaa
     4021 gcttttagaa aagaccggca agcttttcac agatgaagaa ggaagaaaag tattggacca
     4081 ggaaggtttt gaccttgcta tgaaaagccc agtccttgtc ctcacaaggg cagatggcac
     4141 atcactatat cccttaagag accttgcgta ccatatagat aaaaagaagg atgaaaacat
     4201 aatagtgctc ggcgaagacc aaaagctcta ccatcagcag ataaaagcag cgcttagcct
     4261 gataaaattc aaagccccaa gagttgtcca ttattctttt gtgctgcttc aggaaggcaa
     4321 gatgtcgaca aggaaaggga cagtcgtgct tcttgaagat tttatgaaag aagcgctcga
     4381 caaatcaaaa aaagaaataa aacaaaggca tggcgaagtt aaagaggaag atgcaaagaa
     4441 gatagcttac ggcgcaataa aatacgctat cttaaaagtt agcaatgaaa aaaacgtgac
     4501 ttttgattgg caccaggcgc tgaactttga gggagattca ggcccatacc tgcagtatgc
     4561 ctatgcacgg ataagctcca tatttgaaaa acacggagat aaaataaaga aagatgtgga
     4621 ctatagcctg ctaaaagagc cgcaggaatt agagctgata aaaaccctcg ggacttttcc
     4681 agacatcgta aaagaagctg cagagcagct aaagccaaat cttatagcca actacctctt
     4741 ccacctggca tcacaatttt ctgttttcta caatgcatgc cctgttctaa aggcagaaga
     4801 agatttgatg caggcaaggc ttgttttgat agactctgta aggaaagtgc ttaagaatgg
     4861 attaagtctt cttgggattg atgttttgga taagatgtga aatcagcttt aaaaagaggc
     4921 aaataatcct gccattatga tctcagaatg tgttatgcat gttggagtag tactgccttt
     4981 acgttcagag caaggaccgc taagagtctg tcttatcccc caggcaggag gttatattgt
     5041 agctacctat cctctttcaa attcaaataa tccattagct gtacaggaat atggtgactt
     5101 agaatctgcg ataaaggcgc acagggaggt gtttgaagcg ctggaaaaag atggccggtt
     5161 gta
//
LOCUS       DASRUK010000151         9731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_961989, whole genome shotgun sequence.
ACCESSION   DASRUK010000151 DASRUK010000000
VERSION     DASRUK010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 9731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9731
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961989"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..242
                     /gene="rpmC"
                     /locus_tag="VFF28_06550"
     CDS             36..242
                     /gene="rpmC"
                     /locus_tag="VFF28_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018154614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HZX45313.1"
                     /translation="MKAKEIRSLSKEDCKAKLLELRKELMRLNGQIASGTTPKSPGQV
                     KQIKKNIARILTIMNEKQEAIKKI"
     gene            239..547
                     /locus_tag="VFF28_06555"
     CDS             239..547
                     /locus_tag="VFF28_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HZX45314.1"
                     /translation="MSEICTKCGLPKELCVCETIAKESQKIIVKLVKKKFNKMSTVVE
                     GIDEKEINLKDLAKRLKSQFACGGTSKDGRIELQGDHKQRVKEILIKTGFAPETIEVQ
                     "
     gene            564..899
                     /locus_tag="VFF28_06560"
                     /pseudo
     CDS             564..899
                     /locus_tag="VFF28_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194315.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
     gene            964..1365
                     /locus_tag="VFF28_06565"
     CDS             964..1365
                     /locus_tag="VFF28_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HZX45315.1"
                     /translation="MQAIKARVTRALQVSTRVETCDNSGAKVLKIFSVYRNKTVKGRR
                     QAAGVGDLVMTSVVRGKPEMRKQTVLAVIVRQKKEYRRPDGMRVKFEDNAAVVLKDDK
                     GNPKGTIFKGPIAKEATERWPGVAKLARIVV"
     gene            1376..1762
                     /gene="rplX"
                     /locus_tag="VFF28_06570"
     CDS             1376..1762
                     /gene="rplX"
                     /locus_tag="VFF28_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HZX45316.1"
                     /translation="MKKFSRSWKSSTQARKQRKYRYNAPLHLRQKFVSVHLSTELKKK
                     YARRHIGLKKGDKVKILRGQFKKHTGTVDEVNLRKIKVYVSGAEITKKDGSKARYPIH
                     PSNLMITELNLGDKLRQKAIERKAVK"
     gene            1759..2448
                     /locus_tag="VFF28_06575"
     CDS             1759..2448
                     /locus_tag="VFF28_06575"
                     /inference="COORDINATES: protein motif:HMM:NF003312.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4e"
                     /protein_id="HZX45317.1"
                     /translation="MRKHLKRLAMPKSWSSKKKGITYITKPSPGPHPIKLALPIKLIL
                     RDMLKVAFNSKEAKRILHEKDVLVDGRVVNDVKFPIGYMDSIELKNGNAFRVSLHKEK
                     LSLIPIDKSEAGIKLCKIIGKSKIAGKDQLNFDDGRNILYTAGKTAYRVGDSVLISVP
                     GNEIKEHIPLEKGCMVSLIGGKHIGETGHIEDIIQNKVTYKGKDGEIIETLKKYVFVI
                     GKEKPVLKLAQ"
     gene            2459..2962
                     /locus_tag="VFF28_06580"
     CDS             2459..2962
                     /locus_tag="VFF28_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004578114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HZX45318.1"
                     /translation="MRQVRIEKITMNVGAGKDQSRLDKGVLLLKAITGISPVKTFTNK
                     RIPSWGLRPGLPIGCKLTLRRKIAREVLKKLLDAKDNKLTLKQFDPEGNIAFGIHEYI
                     DIPGVKYDPKIGIMGLEVCITFERPGYRVKHRKIMNRKIASSHRIKKEEVIDYMKKEF
                     DVKIGEE"
     gene            2963..3193
                     /locus_tag="VFF28_06585"
     CDS             2963..3193
                     /locus_tag="VFF28_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HZX45319.1"
                     /translation="MTYSNYNKVFKQLRAKPVKLAKYIKHNSPKERKFGPTTKHCRIC
                     GNERGHIGKYGLDVCRRCFRQYATDIGYKKYN"
     gene            3201..3590
                     /locus_tag="VFF28_06590"
     CDS             3201..3590
                     /locus_tag="VFF28_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011751915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HZX45320.1"
                     /translation="MLNDTLSTALSVVMNMEKTGKKECEIRPSSKIIKTVLTIMNENN
                     YIGSFDEVNTARGNLLKVNLLGNINKCGAIKPRYSVEKEDFEKFEKRYLPAKDFGIII
                     ISTSKGIMTHTEAKKKGIGGVLLSYCY"
     gene            3592..4137
                     /gene="rpl6p"
                     /locus_tag="VFF28_06595"
     CDS             3592..4137
                     /gene="rpl6p"
                     /locus_tag="VFF28_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014734781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HZX45321.1"
                     /translation="MGNLKDLKLEIGIPEGVSVKVDNTHVSVKGKSGEASKEMHNPKI
                     KVFAEANKIVIEALKPTRREQRIAYTFNAHIKNLIKGAAESYIYKLKICSGHFPMNVS
                     TKGNELIVKNFFGEKVPRVLKLKERVNVKVEGDIITVTSANKELAGQAAADIEQLTRR
                     TRFDTRVFQDGIWIIEKEGMN"
     gene            4140..4703
                     /locus_tag="VFF28_06600"
     CDS             4140..4703
                     /locus_tag="VFF28_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="eL32 family ribosomal protein"
                     /protein_id="HZX45322.1"
                     /translation="MDSRILKVKEEIKKRQPEFCRQDAHKKRLKIRWVKTRGLHSKVR
                     LRRAGHSTIVSTGFRGPREIRGYSKEGLKIIMLFNEEGASQIDKKNEVAMISGRIGLK
                     KRLAVLKKCTENGIKVLNIDAKEFIAKKEEELKKKAESKKTKTEQKQKKKEEKKKEEK
                     LEEKLSDEEKKEKEKQEKDKLLTQRDI"
     gene            4700..5143
                     /locus_tag="VFF28_06605"
     CDS             4700..5143
                     /locus_tag="VFF28_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19e"
                     /protein_id="HZX45323.1"
                     /translation="MKLDNQKRLAAQVLKCSKKRIKFDTESLGDIKEAITKADIRRLI
                     IGNIIWKENAVGVSRVRARKRIIQRRKGKQKGEGSRKGSRNARLSFKEEWINKVRAQR
                     ELLRSLLDRKAINRQLFRELYLKSKGGFFRSRRHIKLYLEERARK"
     gene            5140..5622
                     /locus_tag="VFF28_06610"
     CDS             5140..5622
                     /locus_tag="VFF28_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008083189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HZX45324.1"
                     /translation="MSIVFKRKRLGKTDYRKRLKLLISSKPRLVVRKSLKHITAQVVE
                     YSSSGDKIITSASSFELKKYGYNGSKGNLPAAYLVGYLAGKKSKEKKVTEIILDSGLY
                     KSVKGSRIYSLLKGCIDAGLSIPCSEEILPSEDRINGKHIKNFDQKNFAESKKKVTEE
                     "
     gene            5624..6448
                     /locus_tag="VFF28_06615"
     CDS             5624..6448
                     /locus_tag="VFF28_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HZX45325.1"
                     /translation="MVDETAEEKVPAEKVTAEEVEDVEKQIVREVKEKAGFDKTSWKP
                     KTSIGMKVKEGEITDIDTILDQGLKILEAEVVDALLPNMNTELLLIGQSKGKFGGGQR
                     RIFKQTQKKTQEGNKPHFAIYACVGDNNGHIGLGYGKSKETVPAREKALRRAKLSMIK
                     VRRGCGSWQCGCREPHTIPFAVKGKCGSCEIELIPAPKGTGLRVEGECQKVLKLAGIK
                     DIWSQTRGQTRTKINLIAACNEALKNLVKMKAADKYFDELGIVEGAKNEQASSNQG"
     gene            6420..6848
                     /locus_tag="VFF28_06620"
     CDS             6420..6848
                     /locus_tag="VFF28_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011153301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HZX45326.1"
                     /translation="MSKLAVIRVRGSIHLRKDIKDTLSLLRLYHQNFCVVSENTPSFT
                     GMVKKVKDYVTFGEIDDATYKELVAKRAEEYKGRLKDAKGKIEYKKFLVVDGKKIKPF
                     FRLNPPAKGFGRKGIKVSFKAKGALGYRADKINDLIKRMI"
     gene            6850..7296
                     /locus_tag="VFF28_06625"
     CDS             6850..7296
                     /locus_tag="VFF28_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011822470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL15 family ribosomal protein"
                     /protein_id="HZX45327.1"
                     /translation="MVVKLRKKFSRQRGRHTHGWGSKKKHRGSGNRGGFGMAGTGKRA
                     DQKNPSIWKDPHYFGKFGFTPQGIKKVINPINIGYLEENIAKFPSKEGVIDLKEFGYN
                     KLLGNGEIKSKIKVKVETASEKAIEKVKEAGGEVICQKSPSARSGL"
     gene            7509..8915
                     /gene="secY"
                     /locus_tag="VFF28_06630"
     CDS             7509..8915
                     /gene="secY"
                     /locus_tag="VFF28_06630"
                     /inference="COORDINATES: protein motif:HMM:NF006341.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HZX45328.1"
                     /translation="MSLWKSIINNLPEVAGPTQKKLSFKEKLKWTLIILVIFFVLGLM
                     PLFGLGQNALQMFEYLSIILAANFGSLVSLGIGPIVTASIVLQLLNGSGIVKFDLTQT
                     EGKKTFQGVQKLLAIFFIIFEAAIYVFMGGLAPNEAFAGTPLYFQMQLILIFQLFLGG
                     MLILFMDEVVSKWGFGSGISLFIAAGVSQQIFVQAFNWMPGPGGLTGVTYSIGAIPAL
                     FQSLAAGDPTTAGIKLAALVATILVFAMAVYAQAMKVEIPLSFGRIRGHGIRWPLSFI
                     YTSNIPVILVSALMANFQLWGRLLQNWFGPNLFARFSEGSGQLVGGFVYWLQAPELVT
                     NLLTHSLTGSQVIQALMYTLFMIAGSVIFSIFWVQTAGMDASSQAKQMMASGLQIPGF
                     RRDQRVLESLLNRYIWPLTIMGAITVGILASVADLSGALSRGTGILLTVMIVYRLYEE
                     IAKQHMMDMYPAMRRFIS"
     gene            8916..9656
                     /locus_tag="VFF28_06635"
     CDS             8916..9656
                     /locus_tag="VFF28_06635"
                     /inference="COORDINATES: protein motif:HMM:NF014061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EMC3/TMCO1 family protein"
                     /protein_id="HZX45329.1"
                     /translation="MVFEGVLSPIFDPLLKLPIFAVVLIMSFLITLITTVIYKYTTNQ
                     SLMKDLKDEMKALQGEMKTLKDNPSKMMEVQKKAMQTNMKYMSHSMRSMLFTFIPIIL
                     IFGWMNSHLAYNPIMPDQQFSTNVQFEKDAQGQVELILPEGISSEGNATQAISEQVKW
                     ILSGKAGGYLLEYKVDGKSYTKDILITNEQRYVAPIKVVKDGTVKQISIDMVKIPVLP
                     LLGWGWLGSYIIFSILFSAILRKLFKVY"
BASE COUNT         3580 a         1694 c         2315 g         2142 t
ORIGIN      
        1 aaagaagaag gaagaagtaa aggaggaagc cgcagatgaa ggccaaggaa ataaggtcgt
       61 tgagtaaaga ggactgcaaa gccaagcttc ttgagctcag aaaagagctg atgaggctaa
      121 acggccagat agcaagcggg accactccaa aaagcccagg ccaggtaaag cagataaaga
      181 agaatatcgc aagaatactc actatcatga atgaaaagca ggaggcaatt aaaaaaatat
      241 gagcgagata tgcactaagt gcggccttcc aaaggagctt tgtgtttgcg agactatcgc
      301 aaaagaaagc caaaagatta ttgtaaagct cgtgaagaag aaattcaaca aaatgagcac
      361 agtggtagaa ggcatcgacg aaaaagagat aaacctcaaa gacctggcaa aaaggcttaa
      421 gagccaattt gcctgcggcg ggacatccaa ggacggcagg atagaactgc agggtgatca
      481 taagcaaagg gtcaaagaaa tactgatcaa gactggtttt gcaccagaaa ccatcgaagt
      541 ccaataaatt tttgagtgaa aaaatggaac aaaaaacaaa gaacatagga atcaaggcag
      601 gaatgcccaa gagggaatgc aaagacagca aatgcccttt ccatggcagc ctgaaatgca
      661 ggggaaaaat atttaccggc gtagtcacat cgaccaagat gcagaaaaca gccacgatcg
      721 aatttaccag gcaatattat attcctaaat atgaaagata tgaaaagaga aggacaaaga
      781 taaaggcgca caaccctgac tgcttaaatg caagggatgg agatatagta agaatatcag
      841 agtgcaggcc gcttggaaaa tcaaaaaatt ttgtagtcat agaaaatctc gggcaagaaa
      901 agggatttgt cataaaaaga gaagctctca tggagagcat gacccgaaag aaggaggaag
      961 aagatgcagg cgataaaagc aagagtgact agagcactgc aggtaagcac aagagttgaa
     1021 acatgtgata actctggagc aaaagtactc aagatattca gtgtatatag aaataagacc
     1081 gtaaagggaa gaaggcaggc agctggtgtt ggcgaccttg tcatgacatc tgtcgtacga
     1141 ggcaagcctg agatgagaaa acagacagtg cttgcagtga tagtcaggca aaaaaaagaa
     1201 tacagaaggc cggacggaat gagagtaaaa tttgaggata atgctgccgt cgtcttaaaa
     1261 gatgacaagg gaaaccctaa gggaaccatt ttcaaagggc ctatcgcaaa ggaggctact
     1321 gaaaggtggc caggagttgc aaaactagca aggatcgtag tttaaggtaa taaaaatgaa
     1381 aaagttttca agaagctgga aatcaagcac acaagcaagg aaacagagga agtacagata
     1441 taatgctcct cttcacctaa ggcagaaatt tgtcagcgtc cacttatcaa ccgaacttaa
     1501 gaaaaagtat gcaagaagac atatcggcct aaagaaagga gataaggtca agatacttcg
     1561 agggcaattc aagaagcata caggtactgt cgatgaggta aacctcagaa agataaaggt
     1621 ttatgtgagc ggagcagaaa taacaaagaa agacggcagc aaggcaagat atccaataca
     1681 tccatctaac ctgatgataa ctgaactaaa cctgggcgac aagctaaggc aaaaagctat
     1741 tgagcggaag gcagtaaaat gagaaaacac ctaaaaaggc tggcaatgcc aaaatcatgg
     1801 tcatcgaaaa aaaagggcat aacatatatc acaaaaccta gccctggacc tcacccaatt
     1861 aagcttgcgc ttccaataaa gctgatactt agagatatgc taaaagtagc cttcaactca
     1921 aaggaagcaa aaagaatcct tcatgagaaa gatgttttgg tagacggtag agtggtaaat
     1981 gatgtcaagt ttcctatagg atacatggat tcgatagagc taaaaaacgg taatgctttc
     2041 agggtaagct tgcataaaga gaaactgtca cttatcccaa ttgataagag cgaagcaggc
     2101 atcaaacttt gcaagataat cggaaaaagc aagatcgcag ggaaagatca gcttaacttt
     2161 gatgatggaa gaaatatcct ttatactgca ggcaagactg catatagagt aggtgactca
     2221 gtgcttataa gcgtgcctgg gaacgagata aaagaacaca tacctcttga aaaaggatgc
     2281 atggtcagcc tgataggcgg aaagcatatc ggcgagacag gacatataga agatataatc
     2341 caaaataaag tcacatacaa aggaaaagat ggagagataa tcgagacatt gaagaaatac
     2401 gtctttgtaa taggaaagga aaaaccagtt ttaaaattgg cacaataaga tgaacccgat
     2461 gagacaagta aggatagaga aaataaccat gaatgtaggt gctggaaaag accagagcag
     2521 gcttgataag ggagtgctgc tcttaaaggc tatcacagga atcagtcctg taaaaacatt
     2581 cacaaacaag agaatccctt catggggcct tagacctggg cttcctatcg gatgcaagct
     2641 gacactaaga aggaagattg cacgagaggt attaaagaaa cttcttgatg caaaagacaa
     2701 caaactcacc ctcaaacagt ttgatcctga aggcaatatc gcttttggaa tccatgagta
     2761 tatcgacata cctggcgtaa aatatgaccc taagataggc atcatgggcc ttgaggtctg
     2821 catcacgttt gaaaggccag gttatagagt aaaacacaga aagataatga atagaaagat
     2881 tgcaagctca catcgaatca agaaagaaga ggtaattgat tatatgaaaa aggaattcga
     2941 tgtcaaaatc ggtgaggaat aaatgacata ttcaaactat aacaaggttt tcaagcagct
     3001 tagggcaaag ccagtaaaac ttgctaaata cataaagcac aattcaccaa aagaaaggaa
     3061 atttgggcct acaacaaagc actgcaggat atgcggcaat gaaagaggcc acatcggaaa
     3121 gtacgggctt gatgtctgca gaagatgctt caggcaatat gcaacagata taggctataa
     3181 aaaatacaac tgaggttaaa atgctgaatg acacactatc cactgcacta tcagtggtga
     3241 tgaacatgga gaaaacagga aagaaggaat gtgagataag gccttcctcc aagattatca
     3301 agactgtcct aactatcatg aacgaaaaca actatatcgg aagctttgat gaggtgaata
     3361 ccgcaagagg caacctgctt aaggtcaacc tgctggggaa tatcaacaaa tgcggagcca
     3421 taaagccaag atatagcgta gagaaagaag attttgagaa atttgaaaaa aggtatctgc
     3481 ctgcaaagga tttcggcata ataatcatat caacctcaaa ggggataatg acccatacag
     3541 aggcaaagaa aaaagggatt ggaggagtac tgctttcata ctgctactaa aatgggcaac
     3601 cttaaagacc taaaactgga aatagggata cctgaaggag tcagtgttaa agtggataac
     3661 acccatgtat ctgtcaaggg aaaatcagga gaagcctcca aggagatgca taatcctaaa
     3721 attaaggttt ttgctgaggc aaacaagata gtcatcgaag cattaaagcc gaccagaaga
     3781 gagcaaagga tagcttacac attcaatgcc catatcaaaa atctaataaa gggtgcggct
     3841 gagagttata tatacaagct caaaatatgc tctgggcatt ttcccatgaa tgtatctacc
     3901 aaaggaaacg agcttatcgt caagaacttt tttggagaga aagtgccaag agtgcttaaa
     3961 ctcaaagagc gagtaaatgt aaaagttgag ggagatataa taactgtaac ttcagcaaac
     4021 aaagagcttg caggccaggc tgctgctgat atcgaacagc taacccgaag aacaaggttt
     4081 gacacaagag tctttcaaga cggtatatgg atcatcgaaa aggaaggaat gaactaaaaa
     4141 tggacagccg aatacttaaa gtcaaagaag aaataaagaa gaggcaacct gaattttgca
     4201 ggcaggatgc ccataaaaaa aggcttaaga taaggtgggt aaagacaaga ggcctgcatt
     4261 caaaggtcag gcttagaagg gcaggccatt ccacaatagt ctcgaccgga tttagaggac
     4321 caagggagat aagaggatac agcaaagaag gacttaagat aatcatgcta tttaacgaag
     4381 agggcgcatc ccagatagac aagaaaaatg aagttgcaat gatatccgga aggataggcc
     4441 ttaagaaaag gcttgctgta ctaaagaagt gcaccgagaa cggaataaag gtattaaata
     4501 tcgatgctaa agagtttatc gctaaaaaag aagaagagtt aaagaagaaa gcggagagca
     4561 agaagacaaa aacagagcag aagcaaaaga agaaagaaga gaagaaaaag gaagaaaaac
     4621 ttgaagagaa gctaagcgac gaagaaaaga aagagaagga aaagcaggaa aaggacaagc
     4681 ttcttaccca gagggacata tgaaactaga caaccaaaaa aggcttgctg cacaggtact
     4741 taaatgctca aagaagagga taaagtttga cactgaaagc cttggtgaca tcaaagaagc
     4801 tatcacaaaa gctgatatca gaagactaat catagggaat atcatctgga aagaaaatgc
     4861 tgtcggcgta tcacgcgtca gagcaagaaa aaggattatc caaagaagga aaggtaaaca
     4921 gaaaggagaa gggtccagga aaggatcaag gaacgccagg ctttctttca aggaagaatg
     4981 gataaacaag gtaagggcgc agagggagct gctgcgatca ttgcttgaca gaaaagcaat
     5041 caacagacag ctttttagag agctgtacct gaaatcaaaa ggcggatttt ttagaagcag
     5101 aagacatatc aaactatacc tggaagagag ggcaaggaaa tgagcatagt attcaagaga
     5161 aaaagacttg gaaagactga ttatagaaaa aggctgaagc ttcttatatc gagcaaacca
     5221 aggcttgtcg tcagaaaaag ccttaaacac atcacagcac aggttgtgga gtattcaagc
     5281 agcggagaca agataatcac ctctgcaagc agctttgagc tgaagaaata tggatataat
     5341 ggaagcaaag gaaatcttcc tgctgcctat cttgtaggat atctggcagg caagaaatca
     5401 aaggaaaaga aggtcactga gataatactt gacagcgggc tttacaaatc agtaaaaggc
     5461 tcaaggatat attcactcct taaaggatgc atagatgccg ggctttccat cccctgctca
     5521 gaagagatac tgccttctga ggacagaata aatggaaagc atatcaagaa ttttgatcaa
     5581 aagaattttg cagaaagcaa aaagaaggtc actgaggaat aaaatggtcg atgaaacagc
     5641 agaggaaaaa gtacctgcag aaaaagtaac ggcagaagaa gttgaagatg ttgaaaagca
     5701 gatagtaaga gaagtaaaag aaaaagcagg ttttgacaag acttcctgga aacccaagac
     5761 cagcatcgga atgaaagtaa aagaaggaga gataactgat atagatacaa tccttgacca
     5821 aggacttaag atacttgagg cagaggtcgt cgatgcacta cttccaaaca tgaacaccga
     5881 actattactt ataggccaat ccaaaggaaa gtttggaggc ggccagagaa ggatattcaa
     5941 gcaaacccag aagaaaacac aggaagggaa caaacctcat tttgctatat atgcctgtgt
     6001 aggagacaac aacgggcaca tcggccttgg gtatggcaag agcaaggaaa ccgtccctgc
     6061 aagggagaaa gcattaagga gagcaaagct ttccatgata aaggtaagaa gaggctgcgg
     6121 ctcatggcaa tgcggctgca gagagccaca taccatccca tttgctgtga aaggaaaatg
     6181 cggatcttgc gagatagagc ttatacctgc acctaaggga acagggctta gggtagaagg
     6241 cgagtgccag aaagtattaa aacttgcagg aatcaaggat atctggtctc aaacaagagg
     6301 acaaacaagg acaaagatca atcttatagc tgcatgcaat gaagcattaa agaacttagt
     6361 aaaaatgaag gcagctgata aatattttga tgaactagga atcgttgaag gagcgaaaaa
     6421 tgagcaagct agcagtaatc agggttagag gatctataca cctaaggaag gatatcaagg
     6481 atacattgag cctgcttagg ctatatcatc agaacttttg tgtcgtctca gagaacactc
     6541 cttcatttac aggcatggta aagaaagtaa aggattatgt cacttttgga gagatagatg
     6601 atgcaaccta caaagagctt gtagctaaaa gagctgaaga gtataaggga agattaaaag
     6661 atgctaaagg aaagatagag tataagaaat tccttgttgt agatggcaag aagataaagc
     6721 cattcttcag acttaatccg cctgctaagg gatttggcag gaaaggaata aaggtatcat
     6781 tcaaagcaaa gggcgctctg ggatataggg cagataaaat caacgacctg attaaaagga
     6841 tgatctaata tggtagtcaa actcagaaaa aaattcagca ggcaaagggg aaggcatacc
     6901 cacggatggg gaagcaagaa gaagcacaga ggctctggaa acagaggagg ctttgggatg
     6961 gctggaaccg gaaagagagc tgaccagaaa aatccaagca tatggaaaga cccccattac
     7021 ttcggaaaat tcggatttac ccctcaagga atcaaaaagg ttataaaccc aatcaacata
     7081 ggctatttgg aagaaaatat cgctaagttt ccttcaaagg aaggagttat tgaccttaaa
     7141 gagtttggat ataacaaatt gcttgggaat ggagagataa aaagcaagat aaaggtcaaa
     7201 gtcgaaactg cttcagaaaa agcaatagag aaagttaaag aagcaggagg agaagtgata
     7261 tgtcagaaat cgccaagtgc acgcagtggg ctttagtcca ttgtaaacaa gcggtttctg
     7321 agtgtctgaa agcttcgctt tctgacacgc aaagaactgc cactgcattc cttgcgccca
     7381 agaaatttag gcataaattt cttagggaac cagaaatttg ctatgcaaat ttctcagttc
     7441 atggtcaaaa actccgctca caccttaatg tggagttttt gacattacca aaaaaagagg
     7501 cctcttaaat gtccttgtgg aaaagcataa taaacaatct gcctgaggtt gctggtccca
     7561 cccagaaaaa actatctttt aaggaaaaac taaagtggac ccttatcatc cttgtcattt
     7621 tctttgttct tggccttatg cccctgtttg ggctggggca aaatgcactg cagatgtttg
     7681 aatatctgtc tatcatcctt gctgcaaatt ttggatcact ggtttcactt ggaatagggc
     7741 caatagtcac tgcatctatc gtgttacagc tgcttaacgg ctcggggata gtcaagtttg
     7801 acttaacaca aactgaaggc aagaaaacgt tccagggagt gcagaagctg cttgcaatat
     7861 tcttcataat atttgaggct gcaatctatg tatttatggg agggcttgca cctaatgagg
     7921 cttttgcagg aacacccctt tactttcaga tgcaactaat attgatattc caactgtttt
     7981 tagggggaat gcttatcctt tttatggatg aagttgtttc caagtgggga tttggttctg
     8041 gaatctccct gtttatcgcc gctggtgttt cccaacagat atttgtgcag gcttttaact
     8101 ggatgcctgg acctggcgga ctgacaggag ttacttattc gataggcgct ataccagcac
     8161 ttttccaaag ccttgctgca ggagatccaa ccactgcagg aataaagctt gctgctttgg
     8221 ttgctaccat acttgtgttt gccatggctg tctatgcgca agcaatgaag gtcgaaattc
     8281 cattatcatt tggaagaata agagggcatg gaataagatg gccattatct tttatctata
     8341 cttcaaatat ccctgtcata ctggtttcag cattgatggc taatttccaa ctatggggaa
     8401 gacttcttca aaactggttt gggccgaatc tattcgctcg tttttcagaa ggaagcgggc
     8461 agcttgtcgg cggatttgtg tattggcttc aggcacctga gctagtaacc aacttactta
     8521 cacattcact aacaggatca caggttatcc aggctctgat gtacacctta tttatgatag
     8581 ctggctctgt gatattcagc atattttggg tgcagacagc agggatggat gcaagcagcc
     8641 aggcaaaaca gatgatggca tctggactgc aaatacctgg atttcgccgt gaccaaagag
     8701 tgcttgaaag ccttctaaac aggtatattt ggccacttac tataatggga gcgataactg
     8761 tggggatact tgcttcggtt gctgatcttt caggagcgct aagcagagga acaggaatat
     8821 tacttacagt gatgattgtt tataggttat atgaagagat tgcaaagcag catatgatgg
     8881 atatgtatcc tgccatgagg aggtttatca gctaaatggt ttttgaagga gttcttagcc
     8941 ctatattcga tccactgctt aagcttccga tttttgcggt cgttctgatc atgtcttttt
     9001 tgattacact tataactaca gtcatctata aatatacaac caaccaaagc ctgatgaaag
     9061 accttaagga tgagatgaag gcacttcagg gagagatgaa gacacttaag gataatcctt
     9121 ccaagatgat ggaagtgcag aagaaggcta tgcagacaaa tatgaaatat atgtctcatt
     9181 cgatgagatc tatgcttttt acatttatcc cgataatatt gatatttggc tggatgaact
     9241 cacatcttgc ttataaccca atcatgcctg accagcagtt cagcacgaat gtgcagtttg
     9301 aaaaagatgc tcagggccag gtagagttga tattacccga gggaataagc tctgaaggga
     9361 acgcaacaca ggctatttct gaacaggtaa aatggatact ttcaggaaaa gctggaggat
     9421 acctgctcga gtataaagta gatggaaaga gctataccaa agacatcctt ataaccaatg
     9481 agcaaagata tgttgctcct ataaaggttg tcaaggatgg aacagtaaag cagatttcaa
     9541 tagatatggt gaaaatacct gtgctacccc ttcttggatg gggatggctt ggctcatata
     9601 tcatattctc aatactattc agtgctattt taagaaagct ttttaaagtg tactagattt
     9661 gcaaatatta tgccaaaacc aagcgacaga tcaaggtcac tccggaagat gtttgtaaag
     9721 acacccgggg g
//
LOCUS       DASRUK010000152         9216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1127338, whole genome shotgun sequence.
ACCESSION   DASRUK010000152 DASRUK010000000
VERSION     DASRUK010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 9216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9216
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127338"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            530..1507
                     /locus_tag="VFF28_06640"
     CDS             530..1507
                     /locus_tag="VFF28_06640"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011229559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HZX45330.1"
                     /translation="MEKKISLIAGTSNSFLANEISSNLNIPLTPIEIKRFNDGEIYIH
                     IEKSVRGSVVFIIQSTSPPVNDKLLELLLIIDACKRASAKEINVIMPYYGYSRQDRKA
                     LPREPISAKLVANLIEAAGANRVITFDLHVEQIQGFFNIPVDNLEAVSLFADYFIKKK
                     LKDIVVVSPDVGGARRARRLAKILDLNIAIIDKRRPAHGVAEIMNVIGEVKGMNAIIM
                     DDIIDTAGTIVGAAKALKKEGAKDIYIVATHPLLSGPASERLMNDAIKEVIVTNTISI
                     SKEKTFEKLRVISLAKLLSDSISKIYEGTPMGVLFDEIYEKIEEKRKAR"
     gene            1504..1914
                     /gene="pfdA"
                     /locus_tag="VFF28_06645"
     CDS             1504..1914
                     /gene="pfdA"
                     /locus_tag="VFF28_06645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit alpha"
                     /protein_id="HZX45331.1"
                     /translation="MNEQQVQEKYIEMKMLQEKVKEMQGQEQLAESQLAELISTVQAM
                     DDFKKAQRGTEILVPISSGIFARAELKDNSKLLVNVGAGVVVSKDIDGAKKLIDDQLN
                     ELGQVHSKISAQVQKLTMYAASIEQELRQMVSKE"
     gene            1917..3203
                     /gene="ftsY"
                     /locus_tag="VFF28_06650"
     CDS             1917..3203
                     /gene="ftsY"
                     /locus_tag="VFF28_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HZX45332.1"
                     /translation="MFKFLKEKLSKGIKSISGQIEEEGKSEEAIIERPVVEEKKGFFS
                     KLKDRFKKEEFPEEEGKEQEVKITTEAEEQKEKVELEQKKEFVEKKEEEIKEKIENKE
                     IPNIQELTELKKEKEIIEKAEEAKKEEQEMPKEEKKGIFGTIKEKIVTKKINDAQFEK
                     IFWDMEVSLLENNIAVEVIEKIKDDLKQELVEKPLPRGKIEEVVQETLYSSLEQILGV
                     QSFNLLEKAKKKKPFVICFVGINGSGKTTTIAKMAKLLQENKLSIVLAAADTFRAASI
                     EQLQLHAEKLGAKLIKHNYGSDPAAVAFDAVKYAQAKNIDVVLIDTAGRMHSNVNLTD
                     EMKKIIRVVNPDLKIFVGESITGNDCVEQAKKFNEAIGIDGIILSKADIDEKGGAAVS
                     IAYVTQKPILFIGTGQEYGDIKEFNPDDVISSLGIA"
     gene            complement(3204..3866)
                     /locus_tag="VFF28_06655"
     CDS             complement(3204..3866)
                     /locus_tag="VFF28_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZX45333.1"
                     /translation="MQLQGIKDKLPERFYLLLQKEITDLRPAQEKAVRQGLLDGKNML
                     VCTPTASGKTLIAELAGVSRILEGKGKMIYIVPLKALASEKHKEFSAKYGRMLNIALT
                     IGDMDSSDSYLSGYDLIICTAEKLDSLIRHHTPWLSSVATVVVDEVHLLNDVGRGPTV
                     EILITILRQLLKNMQLIALSATIGNPKELAEWLGAELVMDDWRPVQLKKGIYLDGKTE
                     FF"
     gene            complement(3893..4555)
                     /locus_tag="VFF28_06660"
     CDS             complement(3893..4555)
                     /locus_tag="VFF28_06660"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZX45334.1"
                     /translation="MRKRVLNAYRDWAPTYDNQVNPSIDLEEKLVVKLMGVKKGDGVL
                     DAACGTGRYSKIFSLKGARVSSVDFSRSMLAVARKKVRGVEFKQADIAKRIPFADKTF
                     DKIICSLAIGHIQRIEPALKEMKRVLKDKGFIVLTALHPDIDFLGFELTGFNFQLSKY
                     KCEMFHKVSVYEKSFAKVGLKVARKMELKINDSIKHCFTERSFKVVKGRPLALVLKLR
                     KV"
     gene            4636..4953
                     /locus_tag="VFF28_06665"
     CDS             4636..4953
                     /locus_tag="VFF28_06665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxC-x17-CxxC domain-containing protein"
                     /protein_id="HZX45335.1"
                     /translation="MRQFTEHKARQERGRKQFGRRSRFNRGESRGFGKQMHTITCAKC
                     GKESEVPFKPTNNKPVYCRDCFNSGAEHSGSRRSDDHRRQSSGLDEINEKLDKIMRAL
                     KIE"
     gene            complement(4954..5307)
                     /locus_tag="VFF28_06670"
     CDS             complement(4954..5307)
                     /locus_tag="VFF28_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45336.1"
                     /translation="MDDIQLPEDELLHKVIQLYVGDRPVLRIGDPLLRHYKILEIVLT
                     ELGITFDRINKKDIWVPKLSGDGYLVAGMGRCVYSDGQLVLTGDSEFYQKGIDIGHME
                     KCKQLRKDVRFMYIE"
     gene            complement(5341..5640)
                     /locus_tag="VFF28_06675"
     CDS             complement(5341..5640)
                     /locus_tag="VFF28_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZX45337.1"
                     /translation="MFKDLNKLMEFPKEGIFSTVLAKSDTYNYTLMCLAAGTDIDEHT
                     STKTGAVIVLKGKGVFRLSGEDIEMKEGLLIFMPANAKHSLKATEDLAILLCLTK"
     gene            complement(5645..6814)
                     /locus_tag="VFF28_06680"
     CDS             complement(5645..6814)
                     /locus_tag="VFF28_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013467120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HZX45338.1"
                     /translation="MEVNMEIARNVFSVGVRDWNRRLFDALIPLPQGTSYNSYLVVGK
                     EKTALIDTVNPGFENELIRNVEEHINIDTLHYLVMNHAEPDHASAIPVVMNKSKAILL
                     TSLKGAEMAKNYYKVPADRIKAVKEGDEIDLGGKTLRFIDAPFLHWPETMFSYLVEDK
                     ILFPCDFFGSHSAFGTYDVDSEDMIPNAKKYFGEIMMPFKTMGKKAMEKLDKMEIKMI
                     APSHGPIHKKPEMIMAEYKKWTNGETRKKVLIAYTSMWGSTENMVNVLRNSLISQGVE
                     VCSYNLAVADIGDIAKDLVDCRAVVLGSPHVLGSVHPLTAYATYLVKALRPPVKYAAF
                     IGSYGWGQGVGKKEVELLGQTDIELVGAIEVNGRPSEEDFAKVIELAHSLCAKLG"
     gene            complement(6805..7635)
                     /locus_tag="VFF28_06685"
     CDS             complement(6805..7635)
                     /locus_tag="VFF28_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZX45339.1"
                     /translation="MKRAIVYIDEEKCNGCGLCVPNCQEGALRIIDGKARLISDLFCD
                     GLGACIGHCPQGAITIEQREARPYDEAKVMAENIVPKGRNTIKAHLEHLKEHGAHEYL
                     NEALAYLKDKGVENPMEEVAGKTFCGCPGARTMSFSTQGEATEEGRRGSQLRQWPVQL
                     HLVSPMAPYFSGKDVVLSADCVAYALGDFHKDYLKGKSLTIACPKLDRDLEEYVNKLV
                     SLIDDSKINSLTVMTMEVPCCQGLLSIATEAARNASRKIQIKSIVVGIKGNIISDEWR
                     "
     gene            complement(7632..7913)
                     /locus_tag="VFF28_06690"
     CDS             complement(7632..7913)
                     /locus_tag="VFF28_06690"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HZX45340.1"
                     /translation="MWQLERHFYLEEKIMMTARGVNEELKGHCDRIISEHEQMIYKAK
                     LIDLSSKDEIRDLIKILNEHQQFEDNFLYPMLDVKLPRPILAQIIERIK"
     gene            complement(7926..8183)
                     /locus_tag="VFF28_06695"
     CDS             complement(7926..8183)
                     /locus_tag="VFF28_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45341.1"
                     /translation="MEKTISDMMNEDHCVIKRLLARFVEAQSLDMSEIAKGAHSFALA
                     KSKPAISECWKSCISNKPEICCANFSVHAQEHAPGFQPSGS"
     gene            complement(8431..9216)
                     /gene="nrdD"
                     /locus_tag="VFF28_06700"
                     /pseudo
     CDS             complement(8431..9216)
                     /gene="nrdD"
                     /locus_tag="VFF28_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872410.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
BASE COUNT         2715 a         2036 c         1909 g         2556 t
ORIGIN      
        1 attagagaaa acacaagtag ctattgatgt gcttacgcac attgtgggca ttcatctacc
       61 ccactaacgt ggggagacct agcccacaaa cttttagtgt tgatccttcg tcttaaaatt
      121 attttggtag aactacttaa tgaatcttac gggagagggt ttctgcaaag cctacacttg
      181 aagcctgaaa aacagcgagg gagatttata aaccgatctt cgtgtctata gaccatgatt
      241 aatttaaatg aggggtcaat agcaagtcaa ggccaggggc aaattttata gggttattgc
      301 cacctgggat gaccttatga aagtaggccc cttattggta agcaaatgca tcaagattct
      361 cagacatgga caatccatac ctaagctagg caagatgatt gataaaagat ctgataatat
      421 gctaaaaatc ctcactaccc gtgtatgcga ccaagagaaa aataaaggac aagtctatgt
      481 cagaggaaag ccgcaatctt tttaaacaat actcgatttt gtttacacaa tggaaaagaa
      541 aataagcttg attgcaggaa catcaaacag tttccttgca aacgagattt cttcaaacct
      601 gaatatccct cttaccccta tagagatcaa aagatttaat gatggggaga tatacatcca
      661 tatagaaaaa agtgtaaggg gctctgtggt ttttataata cagtccactt ccccgccggt
      721 caatgataag cttctggagc ttctgctgat aatcgatgcc tgcaagagag cgtctgcaaa
      781 agagataaat gtgataatgc cttactatgg atattccagg caggacagaa aggccttacc
      841 cagggagcct ataagcgcaa aattggtcgc aaatcttatc gaagcagcag gagcaaaccg
      901 tgttataact tttgaccttc atgtcgaaca gatacaggga ttcttcaaca tcccagtaga
      961 taatcttgaa gcagtatcct tgtttgctga ttactttatc aaaaagaagc tgaaagacat
     1021 tgtcgtggtc tcaccggacg tcggcggtgc aagaagggca agaaggctgg caaagatcct
     1081 tgacttgaac atcgctatca tcgacaaaag aaggccagcc catggcgtcg ctgagataat
     1141 gaatgtcata ggcgaagtca aagggatgaa tgccataata atggatgata taattgacac
     1201 tgcaggaaca atagttgggg ccgcaaaagc actaaaaaaa gaaggtgcaa aagacatata
     1261 tatcgtagcg acgcatccgc tgctttcagg cccagcctct gagcgcctga tgaacgatgc
     1321 gataaaggaa gtcattgtca caaacaccat aagtatatcc aaagaaaaga catttgaaaa
     1381 gcttagggtc atctctttgg caaagctttt gtcggattct atatccaaga tatatgaagg
     1441 tactcctatg ggagttttat ttgacgagat ctatgaaaag atagaggaga aaaggaaggc
     1501 aagatgaacg agcagcaggt gcaggaaaaa tacatagaga tgaagatgtt gcaggaaaag
     1561 gtaaaggaga tgcaaggcca ggagcagctt gcagaaagcc agcttgctga gcttatctcc
     1621 actgtccagg ctatggatga ctttaaaaaa gcccaaagag gcacagagat ccttgttccg
     1681 ataagctcag gcatttttgc aagggcagag cttaaggata attcgaagct tcttgtcaat
     1741 gtgggggcag gagttgttgt ctcaaaggat atagacggcg caaaaaagct tatcgatgat
     1801 cagctaaacg aacttggcca ggttcattca aagatatctg cccaggtcca gaagcttacc
     1861 atgtatgcag caagcatcga acaagagtta aggcagatgg tctctaagga ataaaaatgt
     1921 ttaagttctt aaaagaaaag ctttcaaaag ggataaagag tatttccggc cagatagaag
     1981 aagaaggcaa gtcagaggaa gcgataattg aaagaccggt tgtagaagaa aagaagggat
     2041 ttttctcaaa gctcaaggac aggttcaaaa aagaggagtt tccagaggaa gaaggaaaag
     2101 agcaggaagt caagataacg actgaggcag aggaacagaa ggagaaggtc gagcttgagc
     2161 aaaaaaagga atttgtcgaa aaaaaagagg aagagataaa ggaaaagata gagaataaag
     2221 agatacctaa tattcaagag ctgactgagc taaagaaaga aaaggagatt atcgaaaagg
     2281 cagaagaggc taaaaaagaa gagcaggaga tgccaaaaga ggaaaaaaaa ggcatcttcg
     2341 gcactataaa ggaaaagatt gttacaaaga agataaatga cgcgcagttt gaaaagatat
     2401 tctgggatat ggaagtatcc cttcttgaga acaatatagc tgtcgaggtt atagaaaaga
     2461 taaaggacga cctaaagcag gaattggtcg aaaagcctct gccaagggga aagatagagg
     2521 aggtcgttca ggagactttg tattcatcat tagagcagat tcttggggtg caaagtttca
     2581 accttcttga gaaagcaaag aaaaaaaagc catttgtgat atgctttgtc gggataaacg
     2641 ggtcaggaaa aactaccacc atagcaaaga tggcaaagct cctccaggaa aacaagctct
     2701 ccatcgttct tgctgcagct gacactttta gggcagcttc gatagagcag ctccagctac
     2761 acgcagaaaa gctcggtgca aagctgatta agcacaatta cggctcagat ccagcagcag
     2821 tggcatttga cgctgtaaaa tatgcgcagg caaaaaatat agatgtcgtg ctgatagata
     2881 cagccggaag gatgcattca aatgtcaatc ttacagatga gatgaaaaag ataataaggg
     2941 ttgtaaaccc tgatctcaag atatttgtcg gagaatctat cacaggaaac gattgcgttg
     3001 agcaagcaaa gaaattcaac gaagccatcg gcattgacgg catcatcctt tcaaaggcag
     3061 atatcgacga aaaggggggg gcagcagtgt ctatagccta tgtaactcaa aagccaattc
     3121 tattcattgg cactggccag gaatatggcg acataaaaga attcaaccct gacgatgtta
     3181 taagcagctt agggatcgcc tgatcaaaaa aattcagtct ttccatcaag ataaatccct
     3241 ttcttaagct gcactggcct ccaatcatcc atgacaagct cagcaccaag ccattctgca
     3301 agctcttttg gattgccgat tgtcgcagac aaagctataa gctgcatatt cttaagaagc
     3361 tgtctcaata tggttatgag tatctcaacc gttgggcctc tgccgacatc atttagaaga
     3421 tggacttcat cgacaacaac tgtagcgaca gatgaaagcc atggtgtatg atgtcttatc
     3481 aggctatcca gcttttcagc ggtgcatatg atcagatcat atccggaaag ataagagtcc
     3541 gaagagtcca tatcgccgat tgtaagtgct atatttagca tcctgccata ttttgcagaa
     3601 aactctttgt gcttttcaga tgcaagagct ttgagcggaa cgatatatat catcttgccc
     3661 ttgccttcaa gtattctgct tactccggca agttcggcga taagggtctt tcccgatgca
     3721 gtcggcgtgc agacaagcat attctttcca tcgagcagtc cttgtctgac cgctttttcc
     3781 tgcgcaggcc taagatctgt tatctctttc tgaagtagga ggtagaatct ttcaggaagt
     3841 ttatctttga taccctgtaa ttgcatcaag aattaaaaat ttggccatca atttaaactt
     3901 tccttaattt gagcacaagt gcaagcggtc ttcctttgac aaccttaaac gacctttcag
     3961 taaaacagtg cttgatagaa tcatttatct tcaactccat ttttcttgca accttaagcc
     4021 ctacctttgc gaaagatttt tcatagacgc taaccttatg aaacatttca catttatact
     4081 tcgataattg gaaattaaaa ccagtcagct caaatcccaa gaaatcgatg tcaggatgaa
     4141 gcgcagtaag aacgataaat cccttgtctt tgagcactct cttcatttcc tttaatgcag
     4201 gctcgatccg ttgtatatgg cctattgcaa ggctgcagat tatcttgtca aacgtcttgt
     4261 cagcaaacgg tattctttta gctatgtccg cttgcttgaa ctcaacgccc ctaaccttct
     4321 ttcttgccac agcaagcatg cttcgtgaaa aatcaacgct agaaaccctg gcgcctttca
     4381 atgagaatat cttcgagtac cttccagtcc cgcaagcagc atccaaaacc ccatcaccct
     4441 tcttgacccc catcaacttt acgaccagct tctcctcaag gtcgatagaa ggattcacct
     4501 ggttatcata ggtaggagcc caatccctat atgcatttag cactcgcttt ctcataacaa
     4561 cagattaagg cacaggtgat ataaaacggt ttccttttac atacttttat aaacaaacat
     4621 aggttctata attttatgag gcaattcaca gaacataagg caagacaaga aaggggcaga
     4681 aagcaatttg gaagacgctc aaggtttaac agaggcgaaa gtagaggctt tggcaagcag
     4741 atgcatacta taacctgcgc caaatgcggc aaagagtctg aagttccttt taagccgaca
     4801 aacaataaac ctgtatactg tagggattgc tttaactctg gagcagagca ttcaggatca
     4861 cgcagatcag acgaccatag aaggcaatca tccggacttg acgagataaa cgagaaactg
     4921 gataagataa tgagagcatt aaagatcgaa tgattactca atatacataa aacggacatc
     4981 tttccttagt tgtttgcatt tctccatgtg ccctatatct attcccttct gataaaattc
     5041 actgtcccct gtaagcacca gttggccgtc actataaacg catcttccca tacctgcaac
     5101 aagatatcca tctccagaaa gtttaggaac ccagatgtcc tttttgttaa tccgatcgaa
     5161 agtaatgccc agctctgtca aaacaatttc cagaatttta taatgcctta gtaaaggatc
     5221 tcctatcctt aaaaccggcc tatctccgac atatagctgt ataaccttat gcagaagttc
     5281 atcctctggc agctgaatat catccatgcg gatgccaagg aaagccagaa atataattct
     5341 ttacttggta aggcagagca atatggccaa gtcctctgtt gctttcaaag aatgttttgc
     5401 gtttgcaggc ataaagatga gcaaaccttc tttcatctct atatcttcgc cagacagcct
     5461 gaacacccct tttcccttaa ggactataac tgctcctgtc tttgtagaag tatgctcatc
     5521 tatatcggtc ccagcagcaa ggcacataag cgtataattg taagtatcag acttggcaag
     5581 cactgtgcta aagatgcctt cctttggaaa ctccataagt ttgttcaggt ctttaaacat
     5641 cagatcaccc cagttttgca caaaggctgt gtgcaagttc gataaccttg gcaaaatctt
     5701 cttcacttgg ccttccgttg acttcgatgg ctccgaccag ctcgatgtcc gtctggccaa
     5761 gcagctcaac ctcttttttt cctactcctt gcccccaccc ataagagccg ataaatgcag
     5821 catattttac aggaggcctc aaagctttca ccagataagt ggcataagca gtcaggggat
     5881 gcacagagcc aagcacatgc ggcgagccta aaacaacagc cctgcaatcc acaaggtctt
     5941 ttgcaatatc acctatatcc gctaccgcaa ggttatagct gcagacctca actccctggc
     6001 ttatcaatga gtttctaagt acatttacca tgttttcagt cgaaccccac atagaggtat
     6061 aagcgataag cacttttttg cgagtttctc cattagtcca ttttttgtat tctgccatta
     6121 tcatttcagg ctttttatgg ataggtccat ggcttggagc tatcatcttt atctccatct
     6181 tgtcgagttt ctccatcgcc ttttttccca tagtcttgaa aggcatcatt atctctccaa
     6241 aatacttttt ggcgttaggt atcatatcct cgctatcaac atcatatgtt ccaaaagctg
     6301 aatgcgagcc aaagaaatca caggggaata atatcttgtc ttcaactagg taactaaaca
     6361 tcgtctcagg ccaatgaagg aatggtgcat cgataaagcg cagtgtcttc ccacccaggt
     6421 ctatctcatc tccctccttg acagctttta tcctatcagc aggcactttg taatagttct
     6481 ttgccatctc agcgcccttt agagacgtca aaagtatagc cttgctcttg ttcataacaa
     6541 ctggaatcgc gcttgcatga tccggctctg catggttcat cacaagatag tgcagcgtat
     6601 ctatgtttat atgttcttca acattcctga taagctcgtt ctcaaatccc ggattcacag
     6661 tgtctattag tgcagtcttc tcctttccga cgactaaata tgagttataa ctcgttcctt
     6721 gcggtagagg tatcaaagca tcgaatagtc ttctgttcca gtcccttaca cctactgaaa
     6781 aaacgtttct tgctatctcc atgtttacct ccattcatcg ctgataatat ttccttttat
     6841 ccctacaacg atggatttta tctgaatctt tctggatgca tttcttgcag cctcagtagc
     6901 tatggataat agcccctggc agcaaggcac ttccatggtc atgacagtca gtgagtttat
     6961 ctttgaatcg tcgataagag atactaattt gttaacatac tcttccaggt cgcggtcaag
     7021 ctttgggcat gctatagtca ggcttttgcc ctttaaataa tctttgtgaa aatcccctag
     7081 tgcataagca acacagtcag ctgacaggac aacatcctta ccggaaaaat agggtgccat
     7141 aggagataca agatgcagct gcacaggcca ttgcctaagc tgggaacctc tccttccttc
     7201 ctcggtagcc tctccctggg tagaaaaact catcgtcctg gcgccaggac agccgcagaa
     7261 cgtttttcct gctacctctt ccataggatt ttctactcct ttgtctttca gataagccaa
     7321 agcttcgttt aggtattcat gcgctccatg ctccttgaga tgctcaagat gagccttgat
     7381 agtattcctt ccctttggaa ctatgttttc cgccatgacc tttgcctcat cataaggcct
     7441 tgcctctctc tgttcgatgg ttatcgctcc ctgagggcaa tggcctatgc aggctccaag
     7501 accgtcgcag aacaggtcgc tgataagcct tgccttgccg tcgattatcc tcaacgcccc
     7561 ttcctgacag tttggcacac aaagcccgca cccgttgcat ttttcttcgt cgatatagac
     7621 tattgctctt ttcattttat tctctctatt atctgggcta ggattggcct tggcagcttg
     7681 acatcaagca tagggtagag gaagttatcc tcaaactgct gatgctcgtt cagaattttt
     7741 attagatccc ttatttcgtc ttttgatgat aggtctatga gttttgcctt gtatatcatc
     7801 tgttcatgct cgctgattat cctgtcgcag tgtcccttta gctcttcgtt tactcctctt
     7861 gcagtcatca taatcttttc ttccagatag aaatgcctct caagctgcca caactcttga
     7921 agttgtcaag aaccgctggg ctgaaagccc ggggcgtgtt cttgagcatg aactgagaaa
     7981 tttgcacagc aaatttctgg tttgttggaa atacacgatt tccaacactc agaaatcgct
     8041 ggcttactct tagctaaagc aaaagagtgt gcgcccttag cgatttctga catatcaagt
     8101 gattgtgcct cgacaaacct tgccagcagc ctctttatca cacagtggtc ttcattcatc
     8161 atgtcagata ttgttttttc catacgttaa ctcccttatt atgcccatta ttaaattttt
     8221 tcaatacttg tatgtacata gcatcataag gtcaaaatcc gttctgatga acaattatag
     8281 atgggcgtag caggtccctt acagggatgg gagctctttt tgctaggact ccgtcgagga
     8341 ttttgctatg cacataccca tactttcata aaaaaaccct aagtatggca agggctctag
     8401 ggggatgcgc cagccttagg gatgaaggtt ttaatttgca gtctctttca caagatattc
     8461 ctctctttgc tgaaattcag ctttttttcc aagattccaa ttgctcagtg gcctgaaata
     8521 gcccactatt ctgctgtata tctcacatgg cacctttttt tccatcgccg ctcacctctg
     8581 aaccgtattt taaaagatca ttatccgtat gttgtttagg gcagtattca tgctctcctg
     8641 caacatagcc atgcacaggg catatgctga aggtgggagt tatggtaaaa tagggcagcc
     8701 gaaatccgta ggctatcttc ttgacgaggt ttcttgtagc tctccagtca tttattctct
     8761 ctccaataaa tccatgaaac accgtccctc ctgtatagag cgtctgcagt tcatcttgat
     8821 gccttaaagc ctcaaacata tccattgtat ggtctacagg caagtgtgtc gaattggtat
     8881 agtagggtgc ctcccttgtc ccagcagtta tgatctcagg gtagagctca acatcttttt
     8941 ttgcaaaccg ataggcagta gactcggcag gggttgcttc aaggttatat aggttgcctg
     9001 tttcctcctg gaaatccttt agtttttccc tcatgaaatg aagtgtttct attgcaagcg
     9061 cttttccttc ctcagaatct atccccttgc ctatgaagtt aagcgcagct tcgttcatgc
     9121 cgttcagccc tatggttgaa aaatgctgct tgtagctgcc cagatacgcc ttggcaaatg
     9181 gcataaggcc attcttgagg tttttttcga ccatct
//
LOCUS       DASRUK010000153         8048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_297715, whole genome shotgun sequence.
ACCESSION   DASRUK010000153 DASRUK010000000
VERSION     DASRUK010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 8048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8048
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297715"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VFF28_06705"
     CDS             complement(<1..206)
                     /locus_tag="VFF28_06705"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872548.1"
                     /note="catalyzes the transamination of the branched-chain
                     amino acids to their respective alpha-keto acids; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid aminotransferase"
                     /protein_id="HZX45342.1"
                     /translation="MEETEFIWMDGKFEKWKEARVHALTHTLHYGAGVFEGISCYEAA
                     KGTAIFRLKEHVDRLYYSANTLKM"
     gene            complement(280..1140)
                     /locus_tag="VFF28_06710"
     CDS             complement(280..1140)
                     /locus_tag="VFF28_06710"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HZX45343.1"
                     /translation="MILETVFPIFLIIIIGFLIGKLKKIDLKPLTQLIIYLAAPCLIF
                     SSVSKSDVSLPDFLLVALSMGLVIVISGFILFLIFKTTKSNKLGLYLPMTIGNTGYLG
                     YPIALFAFGAAGLSQAVIADITSSFFLYSAGIFIIHHENDWKEIFKVPLIYAVVGGII
                     YNLLKIPLPLTISRPIEMIGAITIPLALLILGYRLTEIKITSVKTTLLASAFKIAGCL
                     SIAVIIVKLLSITGLSRNIILLQAAMPSAVMSMILTEKYQRDSELVASIILVSTLLSL
                     ITIPLVLWFL"
     gene            complement(1174..2238)
                     /locus_tag="VFF28_06715"
     CDS             complement(1174..2238)
                     /locus_tag="VFF28_06715"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZX45344.1"
                     /translation="MADMRIGALTACGPRMSLEDWMKEANRLGLDGLEVAAFQAGNLG
                     DVASGSRKDYVADTLNVDEPLSDAKAGQIIQMSKELKVPIHALGSYDNTLYVLTGRNN
                     RAHLKKVIDGAAKLREIMPAGPLVATFVGADPALRMQANYELFVDQFIPLIGYAKERG
                     VRIMIENCPMEGWEPSDRPVNNLMSSPRYWNACFKAANEAGVGDAFGLEYDPSHRIWQ
                     LAGRMDLVVRDVETYTQQGKIFAIHGKGAKHDDEGLWTDGVDGRLLDLPHEWAKRSNY
                     EHAVPGVGYDSVQWGKIIPIAKEAGVPFVSIEIEDPTYKDMKDPVRNGILGVQAVEIG
                     RKNLAPICYANGASYQPRKA"
     gene            complement(2256..3575)
                     /locus_tag="VFF28_06720"
     CDS             complement(2256..3575)
                     /locus_tag="VFF28_06720"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZX45345.1"
                     /translation="MVERLNMGVIGAGFIGPVHMRSLASHFEIGVPYAVADSNAQKHE
                     GLKSLGLKVYGSADELIRDSDIQAVHIAGPDGLHAEWALSAMKAPGKKVVVCEKPMTT
                     SLDDALRVMEAADAFEARGGKFMVDLNYMGHALPRAVRELHTNGYFGKRDIMVLANYL
                     QAWLLTNDKLSAKQKMDMGLPDTWNWRLEDDCAKRDILPHLITATYFMTGQYPVRLIA
                     HSAVVVPERIIEKGTGADAFSKGTGAEQKKEYETRPCTSDLYTTVMVEFNTGAVGTYT
                     VTQALPGRKNDWSIIVGGSQRSATWHQKNPNELIIGQSGDLAISPGDAQGKVYDPGNI
                     GDVVIKNDGGDLVKMGCVDAAQYVSDPGEHPWGHRGAMGTQFAQAYLVGLGKKTKDQV
                     VLPGATTGAMSVAVFDAVKESLAGGQQGSWVTVNYKGIDNMLSRGRL"
     gene            3797..3871
                     /locus_tag="VFF28_06725"
     tRNA            3797..3871
                     /locus_tag="VFF28_06725"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3832..3834,aa:Pro,seq:cgg)
     gene            complement(3919..4131)
                     /locus_tag="VFF28_06730"
     CDS             complement(3919..4131)
                     /locus_tag="VFF28_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45346.1"
                     /translation="MADNFFGFRSDKKVVVKISEPEVELSIIKRLGKPKFWVSNNDFY
                     EAMGKIYGAASRKALEICKMDSDKKL"
     gene            complement(4121..4792)
                     /locus_tag="VFF28_06735"
     CDS             complement(4121..4792)
                     /locus_tag="VFF28_06735"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HZX45347.1"
                     /translation="MYWEYFPRAAPRPKPKPGKARKKFGQTWWGEKWVSVLSEFEHDQ
                     RMSRGRAYARADKVKKFKVKKGRIDATVEGSMGDYEVNITFEQFGPKKRAQFIHKVTE
                     VPIIMGRLLNNELPENMDEITGCSIIPSSFESDCSCPDYANPCKHIAAVFYTLADEID
                     YDPTILFKLKGLEKDELLAKAGVLESADDNKRAVAAKKKPGQGKARKAKMKKKNSARR
                     VAYGR"
     gene            complement(4794..7649)
                     /locus_tag="VFF28_06740"
     CDS             complement(4794..7649)
                     /locus_tag="VFF28_06740"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZX45348.1"
                     /translation="MDELQITCIDNKLFLWGISSDSDEMFTPLDRLEGAYSQFKSTPK
                     RQSLELPAIGSVPMVPETMRMFYSQFGASDSKKAFSVAGVQLSDEDLCYRFLGMEKKH
                     GFHFGASYEFFRSYLRFALGLVARQRFVPYSKNSRSCFIANLDNPDDFKSFNDFCSIA
                     PLSIKPKMDGDVKETLRQCLDYLINLVINESDISINIHNETKTDKWLRGLLGKQEKVD
                     LNKEVEEWLSLGKNNRDLGYNFLFKLTEPQNDDMWRLVYNLQSKKDLSLIIPVSEIWK
                     GRKLPVSNIKIHLLQDLGVASKLSKAIEQSLYKPAPSEAVLSANQAMEFISEDSLLLK
                     DAGFTVQIPKITQAKMNSLKVKVRFKDSKLKVSGTGMLGQALFDFDYEVAIGDLDLSR
                     EEFLALSLCKEKLVNVKGKWVEVNPADIKRVVDFFQNRGRLAISDALSPQGINIDSVE
                     VPERFKEEFSGLFNFKDIIKTDPPRDLQGTFRPYQKDGFSWMLFLRNLGFGGILADDM
                     GLGKTIQAIGYLLTQKKPSLVICPTSVLGNWQRELNRFAPALKVHLHYGSERLDKKSF
                     ASVAKNFDIILSSYSTARRDGDILGSVEWDTVILDEAQNIKNPYTKQASAISSIKARA
                     RFCLTGTPVENRLSELWSIMNFCNPSYLASWDSFKRNFAEPIELESDAYKTELLRKII
                     SPFILRRLKTDKHIIDDLPQKTEIKEYCSLTAEQATLYQAVVDDSLKKIKEDSENRRA
                     LIMAALIKLKQVCNHPSNYLKDSTALLDRSGKVERLRELVEVIIGNREKCLIFTQYKE
                     MGELLRADLGNRFDEAVCFLHGQLSKKERELMIDLFQSDDASSPKIFILSLKAGGFGI
                     NLTKANHVIHFDRWWNPAVENQATDRAYRIGQTKNVFVYKFITSGTIEEKIDDMIEQK
                     LSLSCSLLSKGEVSLTELDNETLKELFSLRRESLEEV"
     gene            complement(7701..>8048)
                     /locus_tag="VFF28_06745"
     CDS             complement(7701..>8048)
                     /locus_tag="VFF28_06745"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HZX45349.1"
                     /translation="PAGAPGMPEMLTPTDAIKGAGYKKVALITDGRFSGGTSGPCIGH
                     VEMEAYNKGPIAAVKDGDIIEIDVPNRKLNVKLSDAEIKKRLKEVKAPERKMTPLLET
                     FRQKFIGKNCYGK"
BASE COUNT         2098 a         1962 c         1457 g         2531 t
ORIGIN      
        1 ttcatcttta gggtgtttgc tgaatagtaa agcctgtcga catgctcttt tagcctaaat
       61 atggctgtgc cttttgctgc ctcatagcaa ctgatgcctt caaagacacc agctccgtag
      121 tgcaacgtgt gcgtaagtgc gtgaactctt gcttctttcc atttttcgaa ttttccatcc
      181 atccaaatga actcggtttc ttccattgat accacctatt ttgcgaaggg ttgaattttt
      241 atttaaatat tttttgtcat gggcttaaca tttctgctat tataagaacc ataatacaag
      301 aggaatagtt atcagcgata gaagagtgct taccaagatg atcgaagcta ccagctctga
      361 gtccctctga tatttttctg taagtatcat ggacatgacc gccgagggca tagctgcctg
      421 aagaaggatt atatttcttg ataagcctgt tatagagaga agcttaacaa ttatgactgc
      481 tattgaaagg cagcctgcaa tcttgaaagc agatgccaga agtgttgtct ttaccgaggt
      541 tatcttaatc tctgtaagcc tgtagcctaa aatcagcaat gctaaaggaa ttgttatggc
      601 gcctatcatc tctatcggtc tacttatagt cagcggcaag ggtattttta gcaagttata
      661 gatgatgcct ccaacaactg catatatcaa aggaaccttg aatatctctt tccaatcgtt
      721 ctcatgatgg attatgaaga tgcctgcact gtataggaaa aaggagcttg ttatatccgc
      781 tatgactgcc tgggaaaggc ctgctgctcc aaaggcaaac aatgctatag gatagcccag
      841 gtatcctgtg tttccaatcg tcatgggaag gtaaagccct aacttgtttg acttagttgt
      901 tttgaaaatc agaaataaga tgaagccaga tattactatg actaatccca tggatagtgc
      961 aaccagcaga aaatccggca aggacacgtc gctttttgaa actgatgaaa agataaggca
     1021 gggagctgca agatagatga tgagttgggt caaaggcttt aggtcaatct tctttaactt
     1081 gcctattaag aagcctatga tgattatcaa gaatataggg aagactgtct ctaaaatcat
     1141 aaagaaaaat aaaagaaaga gttatttaaa ggtttaggct tttcttggct ggtatgacgc
     1201 gccatttgca tagcaaattg gggccaaatt ttttcttcct atctctactg cttgcactcc
     1261 aagtatccca ttccttacgg ggtctttcat atccttataa gttggatctt caatctcaat
     1321 ggagacaaaa ggcactcctg cctccttggc tatcggaatt attttgcccc attggacgct
     1381 atcataacca acacccggaa ctgcatgctc ataattgctt cttttagccc attcatgagg
     1441 caaatcaaga agccttccat caacaccatc agtccataga ccctcatcgt catgcttagc
     1501 gcccttccca tgtattgcaa atatctttcc ttgctgagta tatgtctcta catctctaac
     1561 cactaagtcc attctaccag caagttgcca aatcctatgt gatggatcat attctagacc
     1621 aaaagcatcc ccaactccgg cctcatttgc agctttaaag caagcattcc agtatcttgg
     1681 ggaggacatc aggttattaa ctggcctatc agacggctcc caaccttcca tcgggcagtt
     1741 ttcaatcatt atcctaaccc ccctttcttt tgcataacca atcagaggta tgaactgatc
     1801 aacaaaaagc tcatagtttg cttgcatcct caaagctgga tctgctccga caaatgttgc
     1861 aaccaacgga ccagcaggca taatctccct caacttagcg gctccatcaa taaccttttt
     1921 tagatgtgcc ctattattcc gacctgtgag gacatatagg gtgttgtcgt aactacccag
     1981 cgcatggatg ggcactttga gttctttgct catctgtatt atttgaccag cctttgcatc
     2041 ggacaatggt tcgtctacat ttaatgtgtc agcaacataa tccttccttg atccagaagc
     2101 tacgtctcca agattccctg cctgaaatgc agcaacttct aatccatcta atccaagtct
     2161 atttgcctct ttcatccagt cctctaaact catacgagga ccgcatgcag ttaaggcacc
     2221 aattctcata tctgccattt ttctcattcc tccatttata gtctaccccg acttagcata
     2281 ttatcaattc ctttatagtt cacagtcacc caactgcctt gttgccctcc tgctaggctt
     2341 tctttcacag cgtcaaaaac tgcaactgac attgcccctg ttgttgcgcc gggtagtaca
     2401 acttggtctt ttgttttctt tccaagccca acaagataag cttgtgcaaa ttgtgttccc
     2461 atcgctcctc tgtgccccca aggatgttct cctggatcac tcacatattg tgctgcatcg
     2521 acacagccca tcttgaccaa atctccccca tcatttttaa taacaacatc gcctatattt
     2581 ccggggtcat ataccttgcc ttgagcatca ccaggactta ttgctaagtc tccagattgg
     2641 ccaataatca gttcgtttgg attcttttga tgccaggtgg cacttctctg tgagcctcca
     2701 acaataatac tccaatcatt ctttcttcct gggagagctt gggttactgt ataagttcca
     2761 actgctcctg tattaaactc aaccattaca gtcgtataga gatcgctagt gcatggcctc
     2821 gtctcatatt ccttcttttg ttcagcgcct gttcccttac taaaggcatc ggcaccagtt
     2881 cccttctcga ttattctctc cggaacaacc acagctgaat gagcaataag tctaactggg
     2941 tattggcccg tcataaaata tgttgcagtt atcaaatggg gtaagatgtc tctttttgca
     3001 cagtcatctt caagcctcca attccaagta tctggtaagc ccatatccat tttttgtttg
     3061 gcggataatt tatcattggt aagaagccat gcctgtagat aatttgctaa aaccattata
     3121 tctcttttac caaagtagcc attagtatga agctctctta ctgctcttgg taaggcatga
     3181 cccatataat taagatcaac cataaacttg ccgcctcttg cttcaaacgc atcggctgct
     3241 tccataaccc taagagcatc atctagtgaa gttgtcattg gtttttcaca gacgactacc
     3301 ttttttccag gagctttcat cgcactgagc gcccattctg catggagtcc atctggacct
     3361 gcaatatgga ccgcttggat atcagagtcc ctgattaatt catctgcact tccataaacc
     3421 tttaagccta gtgattttaa tccctcatgt ttttgagcat ttgaatctgc cacagcataa
     3481 ggaacgccta tctcaaaatg acttgctaaa gatctcatat gtacgggtcc aataaatcct
     3541 gcacctataa ctcccatgtt tagtcgttca accattttaa catccctcct tcaagattcc
     3601 tttctttatt aaactctaag aaattgctaa ctttaacttg gctttccatt ataccctccg
     3661 gttcttggac aaaccctata tgaaaacagt aacttaggtt acttataaat taatcgacgg
     3721 aaaaatgtcc caagttggtg atgaatttaa aaaaaagtca aattattaaa gaaggaataa
     3781 ttttcctctc aaaaaagggg ctgtggtgca acttggttaa cattcgacgt tcgggacgtc
     3841 gagactctgg gttcgaatcc cagcagcccc actaaatacc cctcctaaat ctctctcaat
     3901 tgatatgggt tacatagttc acagcttttt atctgaatcc attttgcaaa tctccaaggc
     3961 tttccttgat gctgccccat aaatttttcc catcgcctca taaaagtcgt tgtttgacac
     4021 ccagaacttg ggctttccca gcctttttat gatgctaagc tcaacttcag gctcactgat
     4081 tttgacaacc actttcttat ctgatctaaa gccaaaaaaa ttatctgcca tacgccaccc
     4141 gcctggctga atttttcttt ttcatcttgg cctttcttgc ttttccctgt cctggttttt
     4201 tcttcgctgc cacggccctt ttattatcgt ctgcgctttc caagacccct gcttttgcaa
     4261 gcagctcatc cttctccagt cctttcagct taaacagaat cgttgggtca tagtctatct
     4321 catcggcgag ggtgtaaaag acagcagcaa tatgcttgca tggatttgca taatcagggc
     4381 agctgcagtc tgactcaaat gaagagggta taatggagca gcctgttatc tcatccatat
     4441 tctcgggcag ctcattgttc agaagcctgc ccataataat cggcacttcc gttaccttat
     4501 gtatgaactg cgccctcttt tttggcccga actgctcaaa cgttatattc acttcataat
     4561 cccccataga gccctcgaca gttgcatcta tccttccttt cttaaccttg aattttttta
     4621 ccttgtctgc ccttgcatac gcccttcctc ttgacatccg ctggtcatgc tcaaactctg
     4681 aaagcactga aacccatttt tctccccacc atgtttgccc aaattttttc cttgcctttc
     4741 cgggctttgg ctttggcctt ggcgctgccc ttggaaaata ttcccaatac atcttacacc
     4801 tcctcaagag actctcttct taagctgaac agctctttta aggtctcatt gtccagctct
     4861 gtcaatgaaa cctcgccctt agataacagt gaacaggaca aagacagctt ttgctctatc
     4921 atgtcgtcta ttttttcctc gattgtacct gaagtgataa atttgtacac aaaaacattt
     4981 tttgtctggc ctatcctgta agccctgtct gttgcctgat tttcaacagc tggattccac
     5041 cacctgtcaa aatgtataac atgatttgcc ttggtcaggt tgattccaaa ccctcctgcc
     5101 ttaagagaca gtataaatat ctttggggag gatgcatcgt ctgactggaa tagatctatc
     5161 atcaactctc tttctttttt agaaagctga ccgtgaagga aacaaacagc ttcatcaaaa
     5221 cgattcccca agtcagccct gagaagctcg cccatctctt tgtactgggt aaagataagg
     5281 catttctccc tgttgccaat tatgacctca acaagttctc tcagcctttc caccttccct
     5341 gacctgtcaa gaagagcagt gctgtcctta agataattag agggatggtt gcatacctgc
     5401 ttaagcttga ttaaggccgc cataataagc gctcttctat tctcactatc ttccttgatc
     5461 ttcttaagag aatcatcaac gacagcctgg tacaaggttg cctgctctgc tgtcaagctg
     5521 caatactctt tgatctctgt tttttgcggc agatcatcta ttatatgctt gtcggtctta
     5581 agtcgcctga gtataaatgg gctgattatc tttcttaaaa gctcagtctt ataggcatcg
     5641 ctttcaagct cgattggctc tgcaaaattc cttttgaacg agtcccagct tgccaggtag
     5701 gaaggattgc agaaattcat tattgaccat aactctgaga gcctgttttc cacaggcgtt
     5761 ccggtcaggc aaaacctggc ccttgcttta atgctgctta tagccgaggc ttgctttgta
     5821 taaggatttt ttatgttctg ggcttcatca agaataactg tgtcccactc gacactgcct
     5881 aatatatccc catcccttct tgcagttgaa tagcttgaaa ggattatgtc aaaattttta
     5941 gcaacagaag caaagctttt tttatccagc ctttctgaac cgtagtgtag gtgaacctta
     6001 agggctgggg caaatctatt taactccctt tgccagttgc caagaactga agtagggcag
     6061 attacaagcg aaggcttttt ttgggtaagc agatatccaa tcgcctgaat cgttttgcca
     6121 agccccatat catcagcaag gattcctcca aatccaaggt tcctcaaaaa cagcatccag
     6181 gaaaacccgt ctttttggta tggcctgaaa gttccctgca gatccctagg ggggtcggtc
     6241 tttattatat ccttgaaatt aaacaagccg gaaaactcct ccttaaacct ttcagggact
     6301 tcaactgaat ctatattaat cccctgtgga gaaagcgcat cggatatcgc cagcctgcct
     6361 ctattttgga aaaagtcaac aactctcttt atgtctgcag gatttacttc aacccacttc
     6421 ccttttacgt ttacaagctt ttccttacag agggataatg ccaggaactc ttcccgtgat
     6481 aaatccaaat cgccaatggc aacctcatag tcaaaatcaa ataatgcctg cccaagcatg
     6541 ccggttccgc ttaccttaag cttgctgtct ttaaatctca cctttacctt cagagagttc
     6601 atctttgcct gagttatctt tggtatctga acagtaaatc cagcgtcttt cagcaacaga
     6661 gaatcttcag agataaactc cattgcctgg tttgctgaaa gaactgcttc agaaggagca
     6721 ggcttgtaga gggactgctc tattgccttt gataattttg atgcaacacc taaatcctgc
     6781 agcaaatgaa ttttgatatt gcttacaggc aattttcttc ctttccaaat ttcacttaca
     6841 ggtattatca agctcaagtc cttttttgac tggagattat agacaagcct ccacatatca
     6901 tcattttgcg gttctgtaag cttaaataga aagttataac ctaagtctcg gttgtttttc
     6961 cccagggaaa gccactcctc tacttctttg tttaagtcta ctttttcttg ttttccgagg
     7021 agtcccctta gccacttatc agttttagtt tcattatgga tgtttatgct gatatcggat
     7081 tcattgatca ccaaattgat gagatagtct aaacattgcc ttaaggtctc cttaacatca
     7141 ccatccatct ttggctttat tgaaagagga gcaatgctgc aaaagtcgtt aaatgacttg
     7201 aaatcatcgg gattgtcaag gtttgcaata aagcaactcc tgctattctt tgaatatggc
     7261 acaaacctct gccttgccac tagacccaag gcaaatctca ggtagcttct gaagaattcg
     7321 taagaggctc caaagtgaaa gccatgcttt ttttccatcc caagaaagcg ataacataaa
     7381 tcctcatcgc tcaattgaac tcccgcgact gaaaaagctt tttttgaatc agaagcgcca
     7441 aattggctgt aaaacatcct catggtttca ggaaccatag ggactgatcc aatagccgga
     7501 agctcaaggc tttgtctctt gggtgttgat ttaaactgag aatatgcacc ttcaagccta
     7561 tctaaaggtg taaacatctc atcactatct gaagaaatcc cccataagaa caatttgtta
     7621 tctatacagg taatctgcag ctcatccatt cttgatgcct ttgcctgata tgacgggctt
     7681 tgtcatttca cttgacaccc ttatttcccg taacaattct tgccgatgaa tttctgcctg
     7741 aaagtttcta gcagcggcgt catctttctt tctggcgctt ttacttcctt caagcgcttc
     7801 tttatctctg catctgataa tttgacattg agcttcctgt tgggaacgtc tatctcaatg
     7861 atatcgccgt cttttactgc tgcaatcggg cctttgttgt aggcttccat ctcaacatgg
     7921 cctatgcatg ggccggatgt gcctcctgag aaccttccgt ctgtgattaa agcaactttt
     7981 ttgtagcctg cacctttgat tgcgtcagtt ggtgtaagca tctctggcat ccctggagct
     8041 cctgcagg
//
LOCUS       DASRUK010000154         6066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1128132, whole genome shotgun sequence.
ACCESSION   DASRUK010000154 DASRUK010000000
VERSION     DASRUK010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128132"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VFF28_06750"
     CDS             complement(<1..449)
                     /locus_tag="VFF28_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45350.1"
                     /translation="MRRLSFAYHFVYLLLCLLLAYSAVVVAYNLELGHEEEKFKKEAA
                     KAKMYQKFADDELEAAQNQSGLLFMFMGLMSADNDGDNLDLQKEVALGTDDNLIDSDG
                     DEIPDGKDKHPAGGDQVWSIKIPWQHKGSDYSTEIAVNEDTYWYYHD"
     gene            complement(446..1021)
                     /locus_tag="VFF28_06755"
     CDS             complement(446..1021)
                     /locus_tag="VFF28_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45351.1"
                     /translation="MVFLVYDKKQAALFILLPLLLFLLLAPTKDKISSIDIPYETGEI
                     FTLKEPYQELEAYESNEPYQDKGFFTDIIPLEGSVRYFDQKNTTYDARTGEFFEACRD
                     NCTCSKVLQDRCIQCACIELTEKYRHDIVYEKIEKERPVLKYRPVTRYRPVLKFKEVN
                     ATRKVVRLRAENRTSQANWLFGYPVPWRLQI"
     gene            1189..1935
                     /locus_tag="VFF28_06760"
     CDS             1189..1935
                     /locus_tag="VFF28_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HZX45352.1"
                     /translation="MKVCIVMPTYNEANNIARVLDKIHEHIKNDMPNHLTNVLVVDDN
                     SPDGSAEIVKTYQRDHPFVYLLNRNAKQGLGSAYISGFKCALANLQPDVIIEMDADLS
                     HDPSKLKSLVSGIEKGADFVIGSRYVEGGTVPKQWGWHRKLISRGSSYATRMLLGANV
                     RDGAGGYRAIRSEYLRKLDLDRLNVKGYAFQAVILYHVVSLGGKVIEIPIDFVDRESG
                     ESKMQVKDMVEGFWVLLRERVKHKPMKVAS"
     gene            1932..>6066
                     /locus_tag="VFF28_06765"
     CDS             1932..>6066
                     /locus_tag="VFF28_06765"
                     /inference="COORDINATES: protein motif:HMM:NF014924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HZX45353.1"
                     /translation="MTRQTCIFLSLLIAFLPVVMGDIAVSGMGYHPDSFKQAVIYGSA
                     FGNAQIIDASGAVVGEYPITKAVSYDNKPKKCLGDIECSVAYFTDFKKEGKYYIKAGN
                     EKSPEFTISKTVYKDTASVLEKFFEATRQQDSSFHADFHAYGTPRLRAMADGSFIMEA
                     DQASTTLIALGNAYMNSPQVFEGFEEHVKNYLEYLISLQGIKVVDDPSGFRLNENVEA
                     QNAFVPGPTNIKEVDVYLATEPPRILKTLPVKSLCGESSSAGFQQCMDNAKNFYKCQA
                     GEVCLNLTYVEKRGRAEYNSEDMSLSTGWYYEFGCYIDIDLNERLFDDGYNPCLIFKA
                     EKKASSTIRALAAYSTGLPVIKDYYPEMYPSLLERAEKTYSYVLAKNKGEIGDEDNAG
                     YLGAAAFILYDHTKNKQYLAKAYELRTAVTTVFISDDSHGNALYWEQYVLHKKDLETQ
                     GYAYTYQGKKPEEFFHDKIYYDYKDRGYVSLGNNAERAYQLDPMVEFQDNFIQFQNSR
                     YMLMESLYALVAKKYYAGAADFITPVGENQLHWLTGMNRIQYGASANSPQKSISFIFG
                     IGKYPSEYHSRYLMDSGYNSASGGNCIGIRGTSLKYKDGNNYVPFDGVSQILGYNLGT
                     SGNGYGGESSAEFFKKKTYLNGLNYIPGWITGAYDISARNDPLERDTIPNYVDNAGSW
                     EFTETENRIVALALETLAYLDNQYNGEGGNTDIPSANSTEGKMSVNTVPTGADIYING
                     SKEAVSPAIISLSKGYYNITAKKIQYMDAIQGVDLDVGETINLNLVLIRRNGTNITGP
                     SNDTVPDMNNTVNNTANNTIAKTGFVNFTSEPTGALITMDEVFLGNTPFIKKAEAGVH
                     NFSAKYNNDTQKKVFEVIENNTLSYLFMLSNHSIQANNSITRTQTSLELIDGAYHILA
                     GDTASFSVTLKENGTVGWYEAGVLSNTTTGIVHHVSWRPSHLVSANITPANVTAKIGE
                     NSYTWPVLVEFVINPFFSNIDGGADIRGSKDSMINVITNDRIHIFQNLSVDIENAYFR
                     TTDRYYLNRSTGNGETIWKKELTDMQYGDNFVVRAIGYGQVNITFEFGLNRAHERYFP
                     ASSSSSSSSGSSSGGGGSGSGGGSPVGTGSVVYARFDPGLVNTSQSIILLSDVKINYG
                     EIKQVTAKILTPYNDYIQKNLTLAAGTNSYGLWTTNISGLLPGKYYLKEMEITSLAGK
                     TFTNSSIAASFFVEGASLPTEKLSLINIVLNASRVESGSLVSVRLDARDSSGITSVVL
                     TGHTSRDGLFTNNLSLFAGTNQYGTWEADLAASSPDTSFFIDSVTLKNKEQERTYSIS
                     DRSFYVNPAPKSYLTAAAILQTPEQWVKEFIEKPLVPSLIGFSLMIILILLFLGYHYG
                     "
BASE COUNT         1834 a         1254 c         1319 g         1659 t
ORIGIN      
        1 ttatcatgat aataccaata ggtatcttcg tttactgcta tctcggtaga ataatcactc
       61 cccttgtgct gccagggtat ctttatagac caaacctggt cacctccagc tggatgttta
      121 tcctttccat ctggtatctc atcaccgtca gagtctatca gattatcatc tgtgcctaaa
      181 gccacctctt tttgcagatc aaggttgtct ccatcattgt cggcagacat caatcccatg
      241 aacatgaaaa gcaagccgct ttgattttgc gcagcctcta attcatcatc agcaaacttt
      301 tggtacattt ttgccttcgc ggcttctttc ttgaactttt cctcttcgtg ccctaattcc
      361 aggttatagg caaccaccac tgcagaataa gcaaggagca aacaaaggag taaataaaca
      421 aaatgataag caaaggaaag gcgtctcaaa tttgaagcct ccagggtaca gggtagccaa
      481 acagccaatt agcctgagaa gtcctatttt ctgctcttag cctaacaacc tttcttgtag
      541 cattgacttc cttaaacttt aggaccggac gatatctggt caccggccta tattttagga
      601 caggcctttc tttctctatc ttttcataca cgatgtcatg cctgtatttt tctgtgagct
      661 cgatgcatgc acattgtata catctatcct gcagtacctt tgagcatgtg caattatccc
      721 tgcaggcttc aaagaactct cctgtgcgtg cgtcatatgt ggtatttttt tggtcaaaat
      781 atctgacact gccctctaaa gggattatat ctgtgaagaa ccccttgtcc tgataaggtt
      841 catttgattc atatgcttca agttcctgat agggctcttt aagtgtaaaa atttcaccag
      901 tttcataagg gatatctata ctggatatct tgtcctttgt aggtgcaagg agcaagaaca
      961 ataataaggg taaaagtatg aagagtgctg cctgcttttt atcataaaca agaaagacca
     1021 tcaaagcacg tatgttttcc ttttttatat agttaatcta tggctcttta ggtttgaaca
     1081 tccatttgga aatatttata tagataaaag tagtattgat atcgctcaag ggttaatatt
     1141 gtcaataagc ttattttttg gtttttaata ttcttagggg gtgttaattt gaaagtctgc
     1201 atagtcatgc ctacatataa tgaagcaaat aacatagcca gagtacttga caagatacat
     1261 gagcatatca aaaatgatat gcccaaccat cttacaaacg tgcttgtggt agatgacaat
     1321 tctccagatg ggtctgccga gatagtaaag acataccaaa gggaccatcc ctttgtctac
     1381 ctattaaaca gaaacgcaaa gcaagggctt ggttcggcct atatatcagg cttcaagtgc
     1441 gctcttgcaa accttcagcc tgatgtcatc atcgagatgg atgctgacct ttcgcatgat
     1501 ccttccaagc ttaagtctct tgtatcaggc attgagaaag gggctgattt tgtcatagga
     1561 agcaggtatg tagaaggagg gactgttcca aaacaatggg gatggcatag gaagctcata
     1621 agcaggggaa gcagctatgc tacaaggatg cttcttggtg caaatgtcag ggacggagct
     1681 ggaggatata gagctattcg aagtgaatat cttagaaaac ttgatcttga caggctgaac
     1741 gtgaaaggct atgctttcca ggctgtcatc ctctatcatg ttgtgtctct tggcggaaaa
     1801 gtaattgaga tacctatcga ttttgtcgac agggaaagcg gagagagcaa gatgcaggtg
     1861 aaagatatgg tggaaggctt ttgggtgctt cttcgcgaaa gggtcaagca taagccaatg
     1921 aaggtggctt catgaccaga caaacatgca ttttcctgtc gttacttatc gcttttttac
     1981 ctgtggttat gggggatata gcggtctcag gcatgggata ccaccctgac tcattcaagc
     2041 aagcggtaat ctatggcagc gcttttggca atgcgcaaat aatagatgct tctggagctg
     2101 tagttggaga gtaccctata accaaagcag tcagttatga taacaagcca aagaaatgcc
     2161 ttggcgacat agaatgctca gtggcctatt tcactgactt taaaaaagaa ggaaaatact
     2221 atatcaaggc aggaaacgaa aaatctcctg agtttacgat atctaaaaca gtatataagg
     2281 atacagcctc tgtactggag aaattttttg aagccacaag gcagcaagat tcaagctttc
     2341 atgctgattt tcatgcatat ggcactccca ggttgagggc gatggctgac ggcagcttta
     2401 taatggaagc agaccaagca tcaacaaccc ttatcgctct tgggaatgcc tacatgaaca
     2461 gccctcaagt tttcgaagga tttgaggagc atgttaaaaa ttatctcgag tatcttattt
     2521 ccctgcaggg gataaaggtg gtagacgatc cttctggatt taggctaaat gagaatgtag
     2581 aggcccagaa cgcatttgtg cctggaccaa caaatatcaa ggaggtagat gtctaccttg
     2641 caacagagcc tccacggatt ctgaaaactc tccctgtaaa atcattatgc ggggaaagct
     2701 cttcagctgg ctttcagcaa tgcatggaca atgccaagaa tttctataaa tgccaagctg
     2761 gtgaggtatg ccttaaccta acctatgtcg aaaaaagagg cagggctgag tataatagcg
     2821 aggacatgtc cttgtctact gggtggtatt atgagtttgg atgctatata gatatcgacc
     2881 ttaatgagag gctgtttgat gatgggtata atccctgctt gatatttaag gctgaaaaga
     2941 aagcttcttc tacaatacgg gcgctcgctg cttattcaac aggcctgcct gtgataaagg
     3001 actattatcc tgagatgtac ccttctcttc ttgagagggc agaaaagacc tattcttatg
     3061 ttctagccaa gaataaagga gaaataggag atgaagataa cgcaggatat cttggagcag
     3121 ctgcttttat cctatatgac cataccaaaa ataaacaata tcttgcaaag gcttatgagc
     3181 tgaggactgc tgtaactact gtctttatct ctgatgattc gcatggcaat gcactgtact
     3241 gggagcagta tgtcctgcat aaaaaagatc ttgagacaca aggctatgca tatacctatc
     3301 aaggcaagaa gcccgaggaa tttttccatg ataagatata ttatgattat aaggacagag
     3361 gttatgtttc ccttggcaac aatgctgaga gggcatacca gcttgatccg atggtcgaat
     3421 tccaggataa tttcatacag ttccagaaca gcaggtacat gctcatggaa tctctttatg
     3481 ccctggttgc aaagaaatac tatgctggag ctgctgactt tatcacacct gtgggtgaaa
     3541 accagctgca ctggctgact ggcatgaaca ggatacagta tggagcaagc gcaaactctc
     3601 cacaaaaatc cattagcttc atatttggaa tagggaaata tccttcagag tatcattcac
     3661 gctatcttat ggattcaggc tataattcag caagtggcgg gaactgcata ggtataaggg
     3721 gaacctcgct taaatataaa gatggaaaca actatgtccc ttttgacggt gtctcacaga
     3781 tacttggata caatcttggt acatccggaa atggttatgg cggagaatct tctgcggaat
     3841 ttttcaagaa gaagacttat ctgaacgggc ttaactatat ccctggctgg ataaccggag
     3901 catatgatat ctctgcaagg aacgatccgc tcgaaaggga tactataccc aactatgtag
     3961 acaatgcggg ttcctgggaa tttacagaga cagagaacag gatagtggcc cttgcccttg
     4021 aaacacttgc ctaccttgac aaccaatata atggtgaggg aggtaacact gatataccca
     4081 gcgcgaattc taccgagggt aagatgtcag tgaacactgt gcctactgga gcagacatct
     4141 atataaatgg cagcaaggaa gcagtctccc ctgccataat ctccctatca aaaggatatt
     4201 acaacataac agccaaaaaa atccaatata tggatgcaat ccagggggtg gacctagatg
     4261 ttggtgagac gataaacctt aatcttgttc taatcaggag gaatggcaca aacattaccg
     4321 ggccatcaaa cgatacagtg cctgacatga acaacacagt aaataacaca gcgaacaaca
     4381 ctatagccaa aacaggcttt gtcaacttta caagcgagcc aaccggtgct ttgataacca
     4441 tggatgaggt gttccttgga aacactcctt tcataaaaaa ggctgaagct ggcgtgcata
     4501 atttttctgc aaagtataac aatgataccc aaaagaaggt atttgaagtg atagagaata
     4561 ataccctaag ctatctcttc atgctttcca accactctat acaggcaaac aattctataa
     4621 ccaggacgca aactagcctt gagctaatcg atggagctta tcatatccta gctggagata
     4681 ctgcaagctt ttcagtaacc cttaaagaaa atggaacagt cggttggtat gaagcaggag
     4741 ttttatctaa cactaccaca ggaatcgttc atcatgtgtc atggaggcca agccatcttg
     4801 tcagcgcaaa cataactcct gccaatgtaa ctgcaaagat aggggaaaac agctatacat
     4861 ggcctgtgct tgttgaattt gtcatcaacc ctttcttttc taacattgat ggaggagctg
     4921 acatcagagg aagcaaagac tccatgataa atgtcataac aaatgataga atccatatat
     4981 ttcaaaactt atctgttgat atagaaaatg catattttcg cacaactgac aggtattatc
     5041 taaaccgctc tactgggaat ggagagacaa tatggaaaaa agaacttact gacatgcaat
     5101 atggagataa ctttgtcgta agagcgatag gttatggaca ggtgaacatc acatttgagt
     5161 ttgggctaaa tcgggctcac gaaaggtatt tccctgcctc aagctccagc agcagctcat
     5221 ccggcagcag cagtggtggc ggagggagtg gttctggagg cggcagccct gttggaactg
     5281 gctctgtcgt ctacgcacgg tttgatccag gccttgtaaa cacgtcccag tccataatac
     5341 tccttagtga cgtaaagata aactacggag agataaaaca agtcaccgca aagatactta
     5401 caccttataa tgattatata caaaaaaacc ttacacttgc agcaggcacc aactcttatg
     5461 ggttatggac cacaaacata tctggactac ttcctggcaa gtattacctt aaggagatgg
     5521 agataacctc cctggcaggc aaaactttta caaacagctc tattgctgcc tctttctttg
     5581 tagagggggc atctttacca acagagaagc tgtcgcttat caatatcgtg ttgaacgcca
     5641 gcagagttga aagcggctcg cttgtttccg taaggttgga tgcaagggat tcatccggca
     5701 tcacatcagt ggtccttaca ggccacacga gcagagacgg cttgtttaca aacaatcttt
     5761 ccctatttgc aggaacaaac caatatggca cctgggaggc tgatcttgcc gcctccagcc
     5821 ctgacaccag tttctttatc gactctgtca cattgaaaaa taaagaacag gaaagaacgt
     5881 attcgatatc agacaggagc ttttatgtga atcctgctcc aaaaagttat ttgaccgctg
     5941 cagctatatt acaaacacct gagcaatggg tgaaggaatt tatcgagaaa ccccttgtgc
     6001 caagcttgat tggattttca ctcatgatta tccttatttt attattttta gggtatcact
     6061 atggca
//
LOCUS       DASRUK010000155         4969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1469011, whole genome shotgun sequence.
ACCESSION   DASRUK010000155 DASRUK010000000
VERSION     DASRUK010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469011"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            466..1299
                     /locus_tag="VFF28_06770"
     CDS             466..1299
                     /locus_tag="VFF28_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45354.1"
                     /translation="MTPILKDIFKSTIKSLEILYVLEGAKPSARIMVSEKDEAQVEGF
                     VSQKGLSYVKSNFKVVKQDKDKVYSDKGLRVPIDSQTQGYFFAYISKDMEKSLRAREL
                     EEKGMHRELGLMLGYPSCCSQFFFERFSLESQRSNDFTLAALDNSKGITFPYQNNIAA
                     RHFDHALLSHFPCSFNCPKSLEIAKRNLSVIDKHDRELALKMAAFLQTGVLYTEDKGV
                     FLLRNMRIEGNNAYYGEVTSNTKNELYDRLMKSGEIKLIGGNIVVEGLIIKKAGLMNF
                     R"
     gene            complement(1292..1603)
                     /locus_tag="VFF28_06775"
     CDS             complement(1292..1603)
                     /locus_tag="VFF28_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45355.1"
                     /translation="MKRLIWIIAVLLLISQAAAISDMRYTLSGTKLQISYKGIAPYQV
                     EIRGDRNLGSPGAFVTVRTYRNTFTYDLKYLPKRELYYFGAKDTAWSYPYAVSACQTL
                     I"
     gene            1793..2869
                     /locus_tag="VFF28_06780"
     CDS             1793..2869
                     /locus_tag="VFF28_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45356.1"
                     /translation="MSIIHRITEGVKAAYQSLQGSKTYSWSHKEYDSSLIKGIAADYN
                     LLVNNPGITGRPFSSSGAINYIAERRGIGCTAVENALYGRVLSSDRLTAPLKQQLLEK
                     YNSGAMSVNQIAAYFSTPEQKIGRKHVYQILHELNNQGQKVYWKGSNKDGLDEAVQNH
                     EPRTAGVLASEAIANIKSYIGRHKPHVSRRAAATAFAVVLAGAALTAAGTRAIEIIKG
                     TTDRYFVHVYDPNTKDEKTIAASGQMPADESHAALSGGYISDDLSNRLTHKYEGDGIN
                     RAEISDVVASSESDQVSMPSRPEDISLAVPAAAIGFPGAAVLSRQASQQGSEVQKVPS
                     RQVTLIKGTEKITVNFPGKTLAKF"
     gene            complement(2866..3432)
                     /locus_tag="VFF28_06785"
     CDS             complement(2866..3432)
                     /locus_tag="VFF28_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HZX45357.1"
                     /translation="MTYQPNNYEHLLGIKGFSEQLLKNHLTLYQGYVTNTNKLMDSFN
                     AMSDDAKSATPEFAELKRRFGWEFNGMRLHELYFENLTTEAKKPSPELLKKIEDSFGS
                     IEKWGNDLKATATMRGIGWVILYQDQGRLFNAWINEHDTGHLSGCKPILVLDMFEHAY
                     MLDYQLKKADYIEAFFKAIDWEVVNKRV"
     gene            3801..4097
                     /locus_tag="VFF28_06790"
     CDS             3801..4097
                     /locus_tag="VFF28_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45358.1"
                     /translation="MGSITNVSIGSEKKKVEVKISMNYAEYIMLQGNATNVRVFSEDA
                     PSVSSRVRTSGKKGETKYLLVPKPAKKEIAFNSNVTCQIRDIGDKVIIFYVIDK"
     gene            4099..4827
                     /locus_tag="VFF28_06795"
     CDS             4099..4827
                     /locus_tag="VFF28_06795"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HZX45359.1"
                     /translation="MEQIARTKTGIAGFDKLIEGGFPSGSTILLSGSPATGKTILGLN
                     FLYNGAVTFEEPGLYISYEETKENIIKTAKQFDWDFDRLLADKKILIKTFQKEPLVLD
                     TIIADIKRQIKGYGIKRIVIDSLTTLNFRRNAEEADREQHLSEETKVKKFIFGLMDGL
                     SEFKDATKIMISEVEGDDERKLASESVSPFLCDGIIFIKYRTMGGAYSRELVVRKMRH
                     TNNKTGFYPMEITKKGIAVKEQGF"
BASE COUNT         1507 a         1003 c         1076 g         1383 t
ORIGIN      
        1 actaaaaaat ttgccattgc aaatttttgg tgcaccaaaa atcctatgga ttttttagtg
       61 tttcaagcgt caaaaaccac ttaaccgtca tgccaccagt ctatagcgac tgaaaggagc
      121 ggtgactggt ggtttttgac acgaaaaaag cgctccatgc agcaaaaata ccaggctttt
      181 ttggtgccct agaaatccgc agatttccat gactttagca ttggagatgt gtcagaaatc
      241 gcaatgtcat attgctattg ctgtgatttc tgagcacgct caaaaacctt tggtttttga
      301 cgaatggctt tctggctttt ttgtgtccta gaaatttaga tggcaagtgc ggaatcaagc
      361 cccacccttt cagggttgag gtaggcactt gccctataat ttctatgact taaatagctt
      421 ctgttttgcg caagataaat atacctgcgc ttgattactt agggtatgac ccccatattg
      481 aaagacatct ttaaatcaac gatcaaatcc ctggagatcc tttatgtcct ggaaggggca
      541 aagccttctg caaggataat ggtgagcgag aaagatgagg cacaggtgga ggggtttgta
      601 tcgcaaaagg ggctttctta cgtcaagtct aacttcaagg tcgtcaaaca ggacaaagat
      661 aaggtttatt ctgacaaggg actaagagta cctatagatt cccagacaca gggatatttt
      721 tttgcttata tctcaaaaga tatggagaag tcgcttaggg caagagagct agaagagaaa
      781 gggatgcata gggagcttgg cctgatgctt ggctatcctt cgtgctgctc tcagttcttt
      841 ttcgaaagat tttctcttga gagccagcga tcaaacgatt ttaccctggc tgcactagac
      901 aacagcaaag gaatcacttt tccttatcaa aacaatatcg ctgcaagaca ttttgaccat
      961 gcattgcttt ctcattttcc ttgcagcttc aattgcccca agtcgctgga gatcgcaaag
     1021 aggaatctgt cagtaatcga taagcatgac agggaacttg ccctgaaaat ggctgctttt
     1081 cttcagaccg gcgtgcttta tactgaagat aaaggcgttt ttcttcttag gaatatgagg
     1141 attgaaggca acaacgcgta ttatggcgaa gtcacctcaa acaccaagaa tgaactctat
     1201 gataggttga tgaaatctgg agagataaaa ctcatcggag gcaatatagt ggtagaagga
     1261 ttaataataa aaaaagcagg tttgatgaac tttagataag ggtctggcag gctgaaactg
     1321 cgtaaggata gctccaggcc gtgtcctttg caccgaaata atacagttcc ctttttggaa
     1381 ggtatttcag atcataggtg aatgtgttcc tgtatgtcct tactgtcaca aatgcacctg
     1441 gagagccaag gtttctatct cctcttatct cgacctgata gggggctatc cctttatagg
     1501 atatctgaag ctttgtgcca gaaagcgtat acctcatgtc agatatcgcc gctgcctgag
     1561 atatcaatag cagcacggct attatccaaa tcaatctttt cataactatc acctctttga
     1621 ggcatctttt gaaaactgcg ctatttaaag ttcacgatag cttcaaatgg cactcattaa
     1681 gttgtattga ctctctatct aaaagctatt gttaatattt tttagcacta ttagtcaaaa
     1741 tgctaactaa tttatgataa aattattaaa tagttatata tttagcgatt atatgagtat
     1801 catccacaga attacagaag gcgtaaaagc tgcttaccaa tctttgcagg gttcaaaaac
     1861 ttattcctgg tcccataaag aatatgattc ttctctcata aaaggaatag ctgctgatta
     1921 taacctatta gtcaataatc caggcatcac cggcagacca ttttccagct caggggcgat
     1981 caactacatc gctgagcgga ggggaattgg ctgcactgct gttgaaaatg cgctttatgg
     2041 aagagtactt tcaagtgata ggttaactgc acctttgaaa cagcaattgc ttgagaagta
     2101 caactcaggc gccatgtcag tcaaccagat tgcagcatat ttttcaacac ctgagcaaaa
     2161 gataggcaga aaacatgttt atcagatttt gcatgagctg aataaccaag ggcaaaaggt
     2221 atattggaag ggatctaaca aagatggatt ggatgaggct gtccaaaatc atgagcctcg
     2281 aactgccgga gttttggctt cagaggcaat cgccaatatt aaatcatata ttggcagaca
     2341 taagccacat gtgagccgca gggctgctgc aactgccttt gctgttgttc ttgcaggtgc
     2401 tgctttgact gctgcaggga cgagagcaat tgagattata aaagggacga cagacagata
     2461 ttttgtacat gtttatgatc caaatacaaa ggatgaaaag accattgctg catcaggcca
     2521 aatgccagca gatgaatccc atgcagctct cagtggggga tatatcagtg atgacctttc
     2581 aaacaggctt acacataaat atgaaggtga tgggataaat agggcagaaa ttagtgatgt
     2641 ggttgccagc agtgaatcgg atcaagtatc tatgccttcc aggcctgaag atatatccct
     2701 tgctgtgcct gcagctgcaa taggatttcc tggtgcagct gttctctcaa gacaagcttc
     2761 tcaacagggc agcgaggtgc aaaaggtacc ctccaggcag gtaaccctca tcaaaggaac
     2821 tgagaagatt actgtcaact tcccaggcaa aaccctggca aaattctaaa cccttttatt
     2881 aactacttcc caatcaatcg ccttaaagaa tgcttctata tagtcagctt ttttcaattg
     2941 gtaatcaagc atatatgcat gctcaaacat gtctaagaca aggatgggct tacatcctga
     3001 cagatggccg gtgtcatgct cgttaatcca cgcattgaat aatctgcctt gatcctggta
     3061 gagaatcacc caaccaatac ccctcatggt agctgtcgcc tttagatcgt tcccccattt
     3121 ttctatcgag ccaaaactgt cttcaatctt ttttagtaac tcaggcgagg gtttctttgc
     3181 ttctgtggtc agattctcaa aataaagctc atggaggcgc attccattaa actcccaacc
     3241 gaaccttcgc ttaagctctg caaattcagg ggttgctgat tttgcatcat ctgacatagc
     3301 attgaatgaa tccatcagct tatttgtgtt tgtgacgtag ccttggtata aggtaaggtg
     3361 gtttttcaac aattgttcgc taaacccttt tatccctaaa agatgctcat agttgttcgg
     3421 ttggtaagtc atatgtttag attaggcaga attctttata ttttttttgg tagtatcagt
     3481 gataatcctc taatataatc aataaataca ttcgtgacct atcacttttg gtgggcattt
     3541 ttgattgctc aaaactgagt agcagaggca agcagggcaa gagtgctgta caatgcttat
     3601 ccttccaagt ttcctttgct tttttcccct atataatcag cttgctatgg cagtagataa
     3661 tggactatat ggagaagcca gaggcgttag ggataagata ctaaaaattg agcctgattt
     3721 ttctctatgg cctgaaaagg gctgacccta tactacttgc gccaacatat ttatatatcc
     3781 tttatccaag cctagttatt atgggctcaa taacaaacgt cagcataggc agtgagaaaa
     3841 agaaggttga agtcaagatc tctatgaact atgccgaata tataatgctt cagggaaatg
     3901 cgacgaatgt cagggtattt tctgaagatg caccaagcgt ctcatcaagg gttaggacct
     3961 caggaaaaaa aggagagaca aaataccttc ttgtccccaa gcctgccaaa aaggagatag
     4021 cgttcaactc aaatgtcact tgccagataa gggatattgg agataaggta ataatattct
     4081 atgtgatcga caaataaaat ggaacaaata gccaggacaa agaccggaat tgcaggattt
     4141 gacaagctga tagaaggcgg gtttccttct gggagcacaa tactcctttc tggctcacct
     4201 gccacaggaa agactatact tgggcttaat tttctctaca acggcgctgt aacattcgaa
     4261 gagccggggc tatacatctc ttatgaggaa acaaaggaga acatcatcaa aaccgccaag
     4321 caattcgact gggactttga tagattgtta gcggacaaaa aaattctcat aaagacattc
     4381 caaaaagagc cgctggtgct cgatactatc atcgcagata tcaaacgaca gatcaagggg
     4441 tatgggataa agaggatcgt catagactct cttacaacac tcaacttccg aaggaatgct
     4501 gaagaggctg acagagagca gcatctgtct gaagagacaa aggtcaagaa attcatcttt
     4561 ggcctgatgg acggactttc agagtttaag gatgccacca aaataatgat ctctgaagtg
     4621 gagggagatg atgaaagaaa gcttgcaagc gaaagcgttt cgcctttcct ttgtgatggc
     4681 ataatattca tcaagtatcg gaccatggga ggagcatatt caagggagct tgtcgtcagg
     4741 aagatgaggc ataccaacaa caagaccgga ttttatccta tggagataac aaagaaaggc
     4801 atcgccgtca aagaacaggg gttctagatg ctttcaacct cgcaggtaat caacgataac
     4861 cggatagtca tagggaatgg caggttctcc atacatggaa ttgatcacat tgcaatcccc
     4921 ctcaatataa tcgcattgct gcacaaatat tttccttatt cccctatat
//
LOCUS       DASRUK010000156         8349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_783847, whole genome shotgun sequence.
ACCESSION   DASRUK010000156 DASRUK010000000
VERSION     DASRUK010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 8349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8349
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783847"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..783)
                     /locus_tag="VFF28_06800"
     CDS             complement(61..783)
                     /locus_tag="VFF28_06800"
                     /inference="COORDINATES: protein motif:HMM:NF022379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HZX45360.1"
                     /translation="MPKSRIKETEDFVIIGNNKLHVRIDRLALAKDGAYEIHDYKTSN
                     SLPTQDDVDSDRQLAVYAYGIKTMYPDAKEIRLIWHYLAFDKEMQSKRTDEQLEKLKE
                     DVLKVIKEVEACRDYPSKESALCQWCEFRPLCPNYKHLYALEKMQVKEYSQDDGVKLA
                     NEYTALCAEMKEREEKLESLKEELLKFSKQKGLERIYGSDVSVSVKNRRAEKPRGVFL
                     SMLRNRWLTLSQSKRVCALSDF"
     gene            785..1186
                     /locus_tag="VFF28_06805"
     CDS             785..1186
                     /locus_tag="VFF28_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45361.1"
                     /translation="MIRLNSEGIYQRFVPEVRGFQLYKRIWYLKENAPEILFKAYVEA
                     YGLDFKELNPKIREEIETAALGQRIASANQQVEAFVRISMQYPARMEEFVKTMIYSER
                     YGPEFKRIALLEREKYYRQGAVGKRAEPFLS"
     gene            1344..2117
                     /locus_tag="VFF28_06810"
     CDS             1344..2117
                     /locus_tag="VFF28_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HZX45362.1"
                     /translation="MRKIAIFSLKGGVGKTTTAVNLAAGLARSGKKVLLMDLDPQGSV
                     RHCLDADDTKKDMFHLIANGAELHECVTHMGKDLDAVTGSETLNEVDQALGARANKKY
                     ILSLKMEKNKEYDYVIMDCPPNIGTMTLNALIYADEVFVPTTVDVLGYKVLKKTMECL
                     ESFNEQHEDDKVEISKIIPTLYDGRLKVSKDVLKMLQSDFYGIVSHEIKMSSKLKEAP
                     RKKCSIFSYAKSSQSAKDYQELVMEVVNTEKQEVMAESQ"
     gene            2114..2431
                     /locus_tag="VFF28_06815"
     CDS             2114..2431
                     /locus_tag="VFF28_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZX45363.1"
                     /translation="MIKDLAAIKEKEEYKGYKGKFINSQKMTVAYWEIKKDSVLPEHS
                     HPQEQIANIIKGKYLLIIGTNNKVLEPGNVAIIPPNTVHSGRAITNCSIIDVFSPPRD
                     EKK"
     gene            2464..3162
                     /locus_tag="VFF28_06820"
     CDS             2464..3162
                     /locus_tag="VFF28_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZX45364.1"
                     /translation="MEIAKGIEIIDLCLYLKKEDILVISDTHIGYEEALNKQGILIPR
                     FQFKEIIERLEGALFKVTKIVINGDVKHEFGNISDQEWRHTLRLLDFLSRYCDEVILV
                     KGNHDTILGPIAEKRKIRVVDELRVGDVLIAHGDKIPKDISEVKTIITGHEHPAISIS
                     DGVRFELFKCYLKGRWKGKILIVQPSFNLVTEGTDILKERLLSPMLKGDISEFEVFVV
                     DKEVFDFGKIGKLT"
     gene            complement(3253..3423)
                     /locus_tag="VFF28_06825"
     CDS             complement(3253..3423)
                     /locus_tag="VFF28_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010421617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45365.1"
                     /translation="MNLEKSLIVFQGKGIRRLWHNEEWFFSVIDIVGFLTSSTIPRRY
                     WFDLKNKLKDEF"
     gene            3520..4635
                     /locus_tag="VFF28_06830"
     CDS             3520..4635
                     /locus_tag="VFF28_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45366.1"
                     /translation="MLPEVVRGDELLPHVRVSSLSPSQDVADLANIVEVTPEPLKHAL
                     IAYKAGSISLSKDPPFIRSCVQNLAEHADYPSLLIMWSSFWNGGRWCEIQEVIISPDM
                     PGTLVPAFAGYSLKEILGGDLPFGDKGAMLGSIERLVEASPVKYKGFEAMGDEAMNVE
                     YRLPNPPPYKTIGRPDLAMHYYSLANNTGKLLAACEGVLVCGGEHLPKVFEGLQIVYL
                     TGSGDQKARAKVYLHNIAQGEYPGYDKVRIAAAEVINDMQPLKQGLLDAIGKGNVPLT
                     SVYYTLYEKGLVTQEEVVGEMEKCLTSPSHDAYEGLNRSIEWADSLGVVIPRKTMLAA
                     ADSAGLKGNYFARVVQYLEKTEQLQEPERRVFAFLQK"
     gene            4728..5567
                     /locus_tag="VFF28_06835"
     CDS             4728..5567
                     /locus_tag="VFF28_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HZX45367.1"
                     /translation="MELEREIIQLYKKAATSLPEDVERSLKKALAKEKSIANYALSNI
                     LKNVDIARRRSVPICQDTGVPVFYVKVPQGTSQHHIKQSIINATRKATLRVPLRPNAV
                     DVESDLNTKDNIGLSKGALEIEQPIIYIEEWEKGKVRIDLLLKGGGSENIGMTYKLPD
                     KALGANRDLEGVKKCIIDAVFKAQGKGCPPYIVGVAVGGAKDILAKESKKQLLRDFSD
                     ETKLEEELKEGINKLGIGPLGLGGKTTALSVKLKMMHRHPASFFVDISFNCWACRRAT
                     YEG"
     gene            5557..6105
                     /locus_tag="VFF28_06840"
     CDS             5557..6105
                     /locus_tag="VFF28_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012966507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HZX45368.1"
                     /translation="MKAESLLPRKMELGSRVMLSGSIFTARDKAHKFLLESDELKEEI
                     TGGVIYHCGPIVKRGFLSSKIVSAGPTTSERLSSFTPELIKKYKIKAIIGKGGMDDDT
                     LAALKKYGCVYLAAIGGAGALIAERIRSVINTYKEQEFGMPEAIYELEVEDMPLVVAM
                     DAKGNSLYKTVFLQSLSKKQNL"
     gene            6145..6684
                     /locus_tag="VFF28_06845"
     CDS             6145..6684
                     /locus_tag="VFF28_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HZX45369.1"
                     /translation="MRVNKAQRVGVFIDVQNMYYSARALHQAKVDFSQILKESVAGRN
                     LIRAIAYVIKTDIVKDEKTFFDALENIGFEVKAKDLQVFYGGQKKGDWDVGIAMDMIE
                     LAPKLDTAILVSGDGDFVPLLNHLKYALGVRVEVMAFGKSCSGKLIEAADSFINMDET
                     PHRYLRRHHVSDTSKKKAI"
     gene            6650..7453
                     /locus_tag="VFF28_06850"
     CDS             6650..7453
                     /locus_tag="VFF28_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HZX45370.1"
                     /translation="MYPILQKRKLSEGTYLMEVDAPDVAKKAKPGQFLMVRIDEKGER
                     IPLTIADSSKRTITLVFLVVGKTTEQLSGLKKGDELIDVAGPLGNPSKIQSFGNVAII
                     GGGLGIAPIYLIAKALKEAKNRVTTIIGAKSKDHLFWEERFKEISDRVFIATDDGSKG
                     QKGFVSDILKELMQREKLDLVIAIGPPIMMKVISDMTRSRIKTIVSLNPIMIDGMGMC
                     GGCRVSIDGEIKFACCDGPEFNGHKVDWQDLMNRNRTYVEEEKCACGGK"
     gene            7450..7707
                     /locus_tag="VFF28_06855"
     CDS             7450..7707
                     /locus_tag="VFF28_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45371.1"
                     /translation="MSEINFCPCCDAPQHKILLCKDDMFFCKECGNFYRFEGIDMKCP
                     KCSAKEIGKSDFPSPSGEAVFQCKKCKKAFSASEFLKANRR"
     gene            7711..>8349
                     /locus_tag="VFF28_06860"
     CDS             7711..>8349
                     /locus_tag="VFF28_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HZX45372.1"
                     /translation="MKGERVPTREQEQEKRIKNFEEVNFGYNEEEAIFEASRCLQCKV
                     PKCVAGCPVNIDIPKFIGYIKDKKFPMAIKTIKKTNNLPGICGRVCPQENQCEKNCIM
                     GIKNDSLNIGKLERFASQFEDEQKAADNKSKKKIAIVGSGPAGLTCAADLALLGHKIT
                     IYEALHDTGGVLRYGIPEFRLPKKIVDKEVEKIKSLGVEIKKNAIIGKTFTLD"
BASE COUNT         2642 a         1515 c         2007 g         2185 t
ORIGIN      
        1 aaagagcaac acaaaacacc tgttagagaa ggtaaagggc ctatgaaatg ccttgatatg
       61 tcagaaatcg ctaagggcgc acactctttt gctttggcta agagtaagcc agcgatttct
      121 gagcatgctc aagaacacgc cccgaggctt ttcagcccgg cggttcttga cagaaacaga
      181 aacgtctgag ccgtatatcc gttcaaggcc tttttgtttt gaaaacttga gcaattcttc
      241 cttcaatgat tcgagctttt cttctctttc tttcatctct gcacataaag cagtgtattc
      301 atttgcaagc tttactccgt catcttgtga atactccttt acctgcatct tttccagagc
      361 ataaaggtgc ttatagttag gacagagcgg cctaaactca caccattggc acaatgcaga
      421 ttcttttgag ggataatccc tgcaggcctc tacttcttta atgaccttaa gaacatcttc
      481 ctttaatttt tccaattgct catctgtcct tttgctctgc atctctttgt caaaggcaag
      541 gtagtgccag ataagcctta tctcttttgc atccgggtac atggtcttga tgccgtaggc
      601 atagacagca agctggcggt cagaatcgac atcatcttga gtgggcaatg agttagaggt
      661 tttgtagtcg tggatttcat aagcaccgtc ctttgccaat gccagcctgt ctatcctgac
      721 gtggagctta ttgttgccga tgatgacaaa atcctctgtt tccttgatcc tggacttggg
      781 cataatgata aggttaaact cggaaggaat atatcaaaga tttgtgccag aagtaagggg
      841 ctttcaactc tataagagaa tttggtatct aaaggagaat gctcctgaaa ttttgtttaa
      901 agcttatgtt gaggcatatg gacttgattt taaggaacta aatcctaaaa ttagagaaga
      961 aatcgaaacc gctgcccttg gccaacggat tgcatcagca aaccagcaag ttgaggcatt
     1021 tgtcaggatt tccatgcaat acccagcaag gatggaagag tttgtaaaaa caatgatata
     1081 tagtgaaaga tatggacctg aatttaagag gattgctttg cttgaaagag agaagtacta
     1141 taggcaggga gcagtcggta aaagggctga accgtttttg agttagcata attaatatgg
     1201 tttctggaaa gcggtgtctt gttctttgaa ggaatgatta gtaaaaaagt tttatttctt
     1261 ctaaattccg ccttttcgta acatttatat accagtagcc catttcctat acagacattt
     1321 caactaaaaa acgaggggaa actatgagaa agatcgcgat atttagcctt aaaggcggtg
     1381 ttggaaagac gacaactgca gtgaaccttg ctgccgggct tgctagaagc ggaaagaaag
     1441 ttttgttgat ggatctggat cctcaaggaa gcgtaaggca ctgccttgat gccgatgaca
     1501 caaaaaaaga tatgtttcat cttatagcaa acggagctga gctccatgaa tgcgtcacac
     1561 atatgggaaa ggatcttgat gctgtcacag gcagcgaaac acttaatgag gtcgaccagg
     1621 ctcttggggc aagggcgaat aaaaaataca tcctttcatt aaagatggaa aagaacaaag
     1681 agtatgacta tgtgataatg gactgtccgc caaatatcgg tacaatgacc cttaatgcac
     1741 tcatatatgc tgatgaggtg tttgtaccta caacagtcga tgtactggga tataaagtcc
     1801 ttaaaaaaac aatggaatgc cttgaatcct tcaatgagca gcatgaggat gacaaggtcg
     1861 agatctcaaa gataatacct acgctttacg acggcaggct taaggtatca aaagatgtac
     1921 tgaagatgct tcagagcgat ttttatggca tagtaagcca tgagataaag atgagctcaa
     1981 agctcaagga agcgccaagg aagaaatgct cgatattttc atatgcaaag agcagccagt
     2041 ctgcaaaaga ctaccaggag cttgtcatgg aagtggtcaa cactgaaaaa caggaagtca
     2101 tggctgagag ccaatgataa aagaccttgc ggcaatcaag gaaaaggaag aatacaaggg
     2161 atacaagggc aagtttatca attcgcaaaa gatgactgtc gcttactggg agataaagaa
     2221 agactctgtt cttcctgagc attctcatcc tcaggagcag atagcaaaca tcataaaggg
     2281 aaaatatcta ttgatcatcg gaacaaacaa caaggtgctt gaacctggaa atgtggcgat
     2341 aatcccgcct aatactgtgc attctggaag ggcgataacg aactgctcta tcatcgatgt
     2401 gtttagcccg ccaagagatg agaagaagta atgtttttat agaatgttct atcttgaatt
     2461 tttatggaga tagctaaggg aatagaaata atagacctgt gcctttattt aaagaaagaa
     2521 gatatcttag tcatctctga cacccatatc ggctatgagg aagcgctaaa caagcagggg
     2581 atacttatac ctaggtttca attcaaggaa attattgaga ggcttgaggg agcattgttc
     2641 aaggtcacaa aaatcgtcat aaacggtgat gtcaagcatg agtttggcaa catatcagac
     2701 caggagtgga ggcatactct gcggcttctt gactttcttt caagatattg cgatgaggta
     2761 atacttgtga aaggcaacca cgacacaatc cttgggccta ttgcagaaaa aaggaagata
     2821 agggttgtag atgagctgag ggttggtgat gttttgatag cacatggtga taagatacct
     2881 aaagacatat ctgaggtaaa gactataatc accggccatg aacacccagc gataagcata
     2941 agtgacggag tgaggtttga actgttcaag tgctatctta aaggaagatg gaaaggaaag
     3001 atactcattg tgcagccttc atttaatctt gtgacagaag ggactgatat cttaaaggaa
     3061 agattgctaa gccctatgct caagggcgat atctcggagt ttgaggtgtt tgtggttgat
     3121 aaggaggttt ttgattttgg gaaaattgga aagctgacat agtactgtga agcctgacaa
     3181 gaaaataatg ctcaatcttc ttgttttgac ttttccaatc tcttaacctc ttcggcttgc
     3241 tctaaataat tttcagaatt catccttaag tttattttta agatcaaacc agtatcttct
     3301 gggaatagtg ctgcttgtta gaaatcctac aatatctata actgagaaga accattcttc
     3361 attatgccac aatcttctaa ttccctttcc ttggaaaaca atcaatgact tttcaaggtt
     3421 cattccaaat gttgaatgac ctgctccgtg tcctgtgcat atacctatat tctttaaaat
     3481 ataaatcttt ataatcttac tgcgatttta aatacgcaaa tgttgcctga agtagtaaga
     3541 ggggatgaac tattacctca tgtgagggtt tcttcactgt ctccttcaca ggatgtagca
     3601 gaccttgcta atatagttga agtaacgcca gaacccttaa agcatgcatt aattgcatat
     3661 aaggctggca gcatttcgct ctctaaagat cctcctttta tcagatcttg cgtccagaat
     3721 cttgctgagc atgctgacta tccttccttg ttgataatgt ggagcagttt ttggaatgga
     3781 ggaagatggt gtgaaataca ggaagttatt atctcgcctg atatgcctgg cacacttgtc
     3841 cctgcgtttg caggttacag cctaaaagaa atacttggtg gcgatctgcc atttggagat
     3901 aagggggcaa tgcttggcag tattgaacgc cttgttgagg cgtctcctgt caaatataaa
     3961 ggctttgagg ctatgggaga tgaggcgatg aatgttgagt atagactgcc aaatcctcca
     4021 ccatacaaga caattggcag acccgattta gcaatgcatt actattcgtt agcaaataac
     4081 acaggaaaac tgcttgctgc atgtgaggga gtcctcgtct gtggtggtga acatttacca
     4141 aaggtgtttg aagggttgca aatagtatat ttaacaggtt cgggcgatca gaaagctagg
     4201 gcgaaagtat atttgcataa tattgcccaa ggtgagtatc cgggatatga taaggtaagg
     4261 atagcagctg ctgaagttat taatgatatg cagcccctaa aacaaggact tcttgacgcc
     4321 attggaaaag ggaatgttcc tcttaccagt gtttattata cactttatga gaaagggttg
     4381 gtaacgcaag aagaggttgt tggcgaaatg gaaaaatgcc taactagccc aagtcatgat
     4441 gcatatgaag gtctaaatcg cagcatagaa tgggcggatt cacttggagt ggtgattcca
     4501 agaaagacta tgcttgctgc cgctgacagt gcaggactga agggtaatta ttttgcacga
     4561 gtggttcaat atctggagaa aacagagcag cttcaagagc cagaaagaag ggtctttgct
     4621 tttttgcaga aatgataata tgtagggccg ctgtcttttt tctgcgctcg atactgtttt
     4681 tgactatttc aattaattta taaaaagaaa gcataccctc cctttgcatg gagctggaaa
     4741 gggaaatcat acagctatac aaaaaagcag caacaagcct tccagaggat gttgagagaa
     4801 gcctaaagaa agctttggca aaagagaaat cgattgccaa ctatgctcta tctaatatat
     4861 tgaaaaatgt cgacatagca agaagacggt cagtgcctat ctgccaggat accggagtcc
     4921 ctgttttcta cgtaaaagtt ccgcaaggca cttcacagca ccatataaaa cagagtataa
     4981 tcaatgcgac aaggaaagcc acgcttagag tacctctcag gccaaatgct gtcgatgttg
     5041 aaagcgactt gaacactaaa gataatatcg gactcagcaa gggtgctctt gaaatagagc
     5101 agccgataat atatatcgag gagtgggaga aaggtaaggt aaggatagac cttttgctca
     5161 agggcggagg gtctgaaaac atagggatga cttataagct gcctgataaa gctcttggag
     5221 caaacaggga tcttgaaggt gtaaaaaaat gcataataga tgctgtcttt aaggcccagg
     5281 gaaagggttg ccctccatat atcgttggtg ttgctgtcgg gggtgcaaaa gatattcttg
     5341 caaaggagag caaaaaacag ctgcttaggg atttttctga tgaaacaaag cttgaagagg
     5401 aattaaagga aggtataaac aagcttggaa tagggccgct tgggcttggc ggaaaaacaa
     5461 ctgcattatc tgtcaaatta aagatgatgc atagacatcc agcctcattc tttgtcgaca
     5521 tatcattcaa ctgctgggca tgccggagag caacctatga aggctgaaag tcttctgccc
     5581 aggaagatgg aacttggaag tagggtgatg cttagcggca gcatatttac cgcaagggac
     5641 aaagcccata aatttttgct tgaatctgat gagcttaaag aagagataac aggcggagtt
     5701 atatatcact gcgggcctat agtcaagaga ggatttttga gcagcaagat agtgtctgca
     5761 gggcctacta caagcgaaag gctgagttct tttacccctg agcttataaa aaaatataaa
     5821 atcaaggcta taatcggcaa aggcggcatg gatgatgata cgcttgctgc tttgaagaaa
     5881 tatggatgcg tctatcttgc cgctattggg ggggcagggg cacttattgc agaacgcata
     5941 agaagtgtca tcaacacata taaagagcag gagtttggca tgcctgaggc tatctatgag
     6001 cttgaagttg aagatatgcc tctggtagtt gcgatggatg ccaagggaaa cagtctttat
     6061 aaaaccgttt ttttgcagtc tttatcaaag aagcaaaatt tataaactga ccatctttat
     6121 cgataatggg ggatttaaag tactatgaga gtaaacaaag cccagagggt aggagttttt
     6181 atagatgttc aaaatatgta ttattctgcg cgagcacttc atcaggctaa agtcgatttt
     6241 tcgcagatac ttaaggaatc tgtcgctgga agaaacctga taagagctat cgcttatgtc
     6301 atcaagacag atatcgtcaa agacgaaaag actttctttg atgctttaga aaacataggc
     6361 tttgaggtca aagcaaaaga cctgcaggtg ttctatggcg gccagaaaaa gggagactgg
     6421 gacgtcggca tcgccatgga catgatagaa cttgctccaa agcttgacac tgcaatcctt
     6481 gtatcagggg atggcgattt tgtgccactt cttaatcatc taaaatatgc ccttggcgta
     6541 agggtagagg tcatggcgtt tggcaaatca tgcagcggaa agcttatcga agctgcagac
     6601 tcatttatca acatggatga aacaccgcat agatatctaa ggaggcatca tgtatccgat
     6661 acttcaaaaa agaaagctat ctgaaggtac ctatctgatg gaagtggacg ctccagatgt
     6721 cgccaaaaag gcaaagcctg ggcagttctt gatggtaagg attgatgaaa aaggtgaaag
     6781 gatacctctt actatcgcag acagctcaaa gcggaccata actctcgtgt ttcttgtcgt
     6841 gggcaagaca acagagcagc tgtcaggcct taagaaaggg gatgaactta ttgatgttgc
     6901 aggccctctt ggaaatccat caaagataca atcatttgga aatgtcgcga taatcggcgg
     6961 aggccttgga atcgccccga tatatctcat agccaaagcg ctgaaagaag caaagaacag
     7021 agtaactaca ataatcggcg caaaaagcaa ggaccatctc ttttgggaag agaggtttaa
     7081 ggaaatatca gacagagtgt ttatcgcaac tgatgatgga agcaaaggcc agaagggatt
     7141 tgtaagcgac atcctaaaag agcttatgca gcgcgaaaag cttgatcttg tgatcgctat
     7201 cggtccgcct ataatgatga aagttatatc cgacatgacc agaagccgga ttaagacaat
     7261 agtgtcgctt aatccgataa tgatcgacgg gatggggatg tgcggaggat gcagggtgag
     7321 catagacggc gagataaagt ttgcatgctg cgacggcccg gagttcaacg gccataaggt
     7381 agattggcag gacctgatga acaggaacag aacatatgtt gaagaagaaa aatgcgcatg
     7441 tggtggaaaa tgagtgaaat aaacttttgt ccatgctgtg atgcaccgca gcataagata
     7501 cttttgtgca aggatgacat gtttttttgc aaggaatgcg gcaatttcta taggtttgaa
     7561 ggcatagaca tgaaatgccc taaatgcagc gcaaaagaga ttggaaagtc ggattttcca
     7621 tcgccaagcg gagaagcggt gttccagtgc aagaaatgca agaaagcgtt ctcggcatca
     7681 gaatttttga aagcaaacag gagataatag atgaagggag aaagagtacc aacaagggag
     7741 caggagcaag aaaagaggat aaagaacttt gaagaggtca atttcggcta taatgaagag
     7801 gaagcaatat ttgaagcttc aagatgcctt cagtgcaaag ttcccaaatg cgttgctggc
     7861 tgccctgtca atatagatat ccctaagttc atcggctata tcaaagataa gaaatttccg
     7921 atggcaataa agaccatcaa gaagacaaac aacctgccag ggatatgcgg cagggtttgc
     7981 ccccaggaaa accaatgtga aaagaactgc ataatgggga taaagaatga ttctcttaac
     8041 atcgggaagc tggagagatt tgcctcccaa tttgaagatg agcaaaaggc agcagataat
     8101 aaaagcaaaa agaagatagc tatagtcggc tcagggcctg cagggcttac ctgcgctgct
     8161 gatcttgcgt tgcttgggca taaaataact atctatgagg ctttgcatga tactggagga
     8221 gtccttaggt atggaattcc ggagtttagg ctgcctaaaa agatagtgga caaggaagtt
     8281 gagaaaataa agtcacttgg cgttgagata aagaaaaatg ctataatcgg taagacgttc
     8341 actcttgat
//
LOCUS       DASRUK010000157        17338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1641550, whole genome shotgun sequence.
ACCESSION   DASRUK010000157 DASRUK010000000
VERSION     DASRUK010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 17338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17338
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641550"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            410..562
                     /locus_tag="VFF28_06865"
     CDS             410..562
                     /locus_tag="VFF28_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45373.1"
                     /translation="MKKGSGTKERISITLDKGLLKEIDKRCEQRLMKVSNYIEKLIKV
                     GLKNEK"
     gene            552..4316
                     /locus_tag="VFF28_06870"
     CDS             552..4316
                     /locus_tag="VFF28_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45374.1"
                     /translation="MKSKNADACLKPFEGPKSRALNQPLLSKIAMFMIALILSIPFYS
                     AAAFAGLSNAAIRSPNSDDPPNYIRKTGTNTISVDAYIEGAAEIDPSQVRLTQKEGSP
                     FEDCQLGLNGKKWRCTYPIESSSLPNGDFQRMVFLLNKSGSDEGSIRIKGIKDAVGPE
                     ITSFTASQATIGKGTITFNYDITDYMSEADSSNCIGLKDIIFTAGAYTNPIEINSQSG
                     ECTYSDTFTEDIEKITKQSSGNITIRAIAHDNFNQQSKIKELSLKIDRQQPQIKEGTF
                     RLLDDNNKDIRFASGTQAAKASIEVADTDLALVKADFTSLSPRNYVMDAECQQGVCTW
                     SGISVSKGGTHQVKITAQDSLGNEQPATILYQIDIDDQKPDVKGLKSNHQDSEGKNYL
                     GKADNQLIYQIEETGVGFGKRNIYLDLRNLGLGENVQAGNCTKTDSSWSCYWKLPALS
                     ASDGAKQIFIRPHSTDDLGNEITGILENEIIVDTIAPKIGTIESSKEAVRGQPLIFTF
                     TASDQNPLIVYANTSKISAVALHTGTCQESCRIEITDLVPSYTVQEIPITAQDVAGNQ
                     AEKKSRITILQADANTTPSFYRIERIEVIPPKVDKQVISQIPVNIFVHAELEGIGSAE
                     IISKTVVCPAMADYLAGTSIIGEEDTYIGLKLNQRAATLPSVPIACSLRLLVKDNKNV
                     YSKYEEENITAQLELYGNSLGDLTETTKSKLDSINQEISNTEDAIKSYEKWIKTWGIW
                     CQISEGLGFYSSLVQTIKSAIWMVLAAKWAACAAGLGSCTKAVDKVWGVKCTSASLPH
                     AHVIEKWIWPSGPIGFPVGMVNKWACSIGFHCAVCNWNTWYDLGVSLVVHTAAKKVGT
                     YDDGKTVREMDRVTVDVEGVNDRGTVESIEGDNMVIKLDNGHTVTKPASQVARVGIDD
                     NVDISINRPDGSVEKIQAKVRDVDLSSGEVTTTQGSYSLTDVQLSDSVEQKPNQIIGR
                     PGEKVSVASSQNKRPSQEGITTDWSIPKKTNQINSPVNAPKGKVEFKNYKTIEDTDSI
                     IIFPGETQARYASTGQAYTYTGKDDKGNEIWQRTYSNYEEQGQLTSVTPSSNWIFDPY
                     KSIHYAKACLCLPAIVYNLKKEKQIQCMQRNCISNHIQKGLPATECDAAYKERQCLYV
                     EGAEYKKHGYGAIAGSLWTFVKNNLPYLAASIGYLATCWDYIISPEGICSTALVVGTP
                     AGGIKPTACGLIGSSMTLMEMASTIQSGFSFLNYDQDLKGEDYCATGNYQ"
     gene            4294..4785
                     /locus_tag="VFF28_06875"
     CDS             4294..4785
                     /locus_tag="VFF28_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45375.1"
                     /translation="MRPEITSKKVIRTIFILILLTAMSRQSASFNPRIDSWYSVEQWE
                     VDLCSKWGGTYEAQSEATSSTGISLSQTTLSLQGRKQDYNIKGVNESLYTLSWYLEPL
                     AEMSYTIELTDDDEIKTYRIDSGQASFAKPAGGYKSEYYSEDYTYLKMTYGTNWLRVP
                     LVQ"
     gene            4782..5528
                     /locus_tag="VFF28_06880"
     CDS             4782..5528
                     /locus_tag="VFF28_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45376.1"
                     /translation="MKKIFQVLALNIAYLIILLAIISLFSFAVRENLSSMANISDPLA
                     KISQMMAGAIEPDSIILKDMAAVSSSIRWFFATMIAVAILTIIAACLTAGYFKAWIWS
                     KITSIPLKKYAALKLSFLLGLWTLSWLLVSIFLVFTLKFSRDVLIKINLIYFILFAYF
                     SLLLIPVFIRKQQIIGSLKETFRLGTKKFYEFIPAFALIGLVAILSFATAIYLAMIYS
                     PLGLIEIPLFLFFIVWTKYLINNKFTRVCR"
     gene            5525..8176
                     /locus_tag="VFF28_06885"
     CDS             5525..8176
                     /locus_tag="VFF28_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45377.1"
                     /translation="MKKAQITVFIIMGFLILLAGIAIFYFTRESVKSDLSSEVEKAQD
                     LPLWAAEVKPYVDKCIQDLSIKAFNKIGEHGGYIDLNDSYLTPSVLSLNPSDPTEADA
                     VAISDKPASSIAYWWYMQTQNTCDDCIVSSNDPPIDMIEWQVARYVDRQLPDCLQNFT
                     VLNGISVKRGSINSTVTINEKDVDVQVLMPTQISNVDMSASIEKYKITLPLDFNHAYL
                     AAALIANHEISERFLEGIVNHIITISSFQDQDKLPPISAIDSKNGRTTWRKSEVKRRI
                     VDDVLLSRIPALQIKNTKTYTQLSSQDPLTQGLNDIMALDILPEGYENLEVSFIYNPD
                     WNIFFDITPSSGEVLGPLTTRTDDTGIGFFPQSVNNFYEFYYDLSFPVLVMIKDPDSL
                     KDQQGYQFIFALEANIRDNKDLYQWNHGNGTIGQADYRRVSLAAKSSQVDAKACEQEG
                     SKWRCPLDDQVYSDMPKCIDKCKTSTQQTKDMTLLPSLFCDSNQRISGNITVNAYDAK
                     TLLPLPGVQLTFGCGNYRKCPMEGTDSQGTYISRFPVCIGDGYIRAEKEGYMPEYEGG
                     VSISPETKTRYDIYLEPIRTKKIEAAYINITNIYRIKNTLHPKTGESMLDELYYKKFE
                     TAQGAQITIHQGILGEIYYKVDDKEKRTVLLKARAIENARDLLKDIEYHQEAKKEPVV
                     DVFFSVKEAAEFAGARSDLLSFVSKSKVDSYIDWFKKEENNIQFRSDTYFSSVIGMAR
                     SQANSLKDKQVLTITYQKAKEKPYEQNMPSSIAQLGNFNTTISLVPGIYNLNMMFRDD
                     QGYVIPAKDGYSEFNYTPAILGGAQLDEKTSQWVIDKERLDASSTIKFYFFKIDNPTS
                     LEDTGEIGSIVNYSIDYRPYIEPEFLP"
     gene            complement(8173..9420)
                     /locus_tag="VFF28_06890"
     CDS             complement(8173..9420)
                     /locus_tag="VFF28_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZX45378.1"
                     /translation="MEKEKLRQVLTEQNKFRDGKLVPRELIRKIDTFEKTPFIIIVSG
                     IRRAGKSTLLHELRAKRRESYYVNFDDDRLIDFHVDDFQTMYELLIELFGKKNTFFFD
                     EIQNINSWERFVRRLNDNGSKVFITGSNASMLSKDLGTHLTGRNISFSLYPFSFREFL
                     LFKDFEPSLDNLTSEKKGQIKKLFNEFFEKGGFPEYLQTEKEEYIKSVYENILYRDIV
                     SRYKLPTERSLKETVYYAASNIGKEISFNSIRKLAGLTSATTIREYFEYLENSYLAFL
                     IPRYSASLNNQIYSNKKVYLVDNGMVKLLGFRPSEDKGRILENIVFIELKRKGKEIFY
                     HRDKYECDFIMRDGPRISQAIQVCYSLEADREVNGLLEAMEKYRLREGLILTDDTEKE
                     ISSGSKKIIVKPVWKWLLEDEQA"
     gene            complement(9611..10798)
                     /locus_tag="VFF28_06895"
     CDS             complement(9611..10798)
                     /locus_tag="VFF28_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45379.1"
                     /translation="MVQNSLVKYIREQIRAGYSAGEIKGYLTKYGYSESQINEAFQFA
                     YPPTEVKHVITFSKRTIALIIAIICTLVLSGAGVYMFVIKEKPDMQLLDVQMDVITST
                     VNQGESLKFTAQIFNLGKSKRFDVFMKYEVYDNTERVLAFKEETLAVETRASSTVNIG
                     VGDIKPGNYYVRAVASYGGKKARATNAFRIQAAAEPSIRQQPDVPKRSCPSSCEDKNK
                     CTTDYCSADTGYQCEHSKVAQCCGNGLCENGEDSNSCLEDCASDLPQDSFGDRPVWEQ
                     IDIIGNIAKNDKPRAIADCKKIEQASFRFDCMTKVAAGTRDESVCDNIEDEPSRDSCI
                     KEVAVQNEDSTICAKMVKDSRRDQCYMDFATKGDYSVCEKLTNMYLKQSCESLKMMSE
                     TSI"
     gene            complement(10785..10934)
                     /locus_tag="VFF28_06900"
     CDS             complement(10785..10934)
                     /locus_tag="VFF28_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45380.1"
                     /translation="MSKKITKERCNFTIDSKLVFRFREYCKENCINMSAKIEKFIEEE
                     LDGAK"
     gene            11054..11554
                     /locus_tag="VFF28_06905"
     CDS             11054..11554
                     /locus_tag="VFF28_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45381.1"
                     /translation="MRNVVVIYGPNEKLGMAVADSFRDRSDLTNLFFYAAQNSRQLSD
                     IEMANITSTARKLGDYVVADLQLLILNAHGAVKPGKAPLNVGTFGYDISPSRGVYPTE
                     LYVNETPFGDQPYMRISAIVSSSDLNGKEIQRKARLIHGLLDRVARQEKGDVVAALQR
                     RKKEAA"
     gene            complement(11653..12057)
                     /locus_tag="VFF28_06910"
     CDS             complement(11653..12057)
                     /locus_tag="VFF28_06910"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HZX45382.1"
                     /translation="MNKDKVFIEHILQEIGKIERTLNRISQQQFREDVDIQDATLRRI
                     EIIGEAVKHISSQLKERYPDIEWKNVRNFPSSLGWMPQSKGFIARTKFLSMLKNRLTV
                     CPTLAFPLPITGVLDRLQGQGTYLSMLILQLT"
     gene            complement(12162..12341)
                     /locus_tag="VFF28_06915"
     CDS             complement(12162..12341)
                     /locus_tag="VFF28_06915"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZX45383.1"
                     /translation="MGMTMDTRMRKTIVAILRKNDVVKAGIFGSYARGEATKSSDIDL
                     LIKFRGKKACLTLLG"
     gene            12820..12984
                     /locus_tag="VFF28_06920"
     CDS             12820..12984
                     /locus_tag="VFF28_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45384.1"
                     /translation="MSQQIRITVSDEIDRLLSKVAKRLGKKKSMLARELMEQKLYDLN
                     LIQRGFEDLK"
     gene            12981..15068
                     /locus_tag="VFF28_06925"
     CDS             12981..15068
                     /locus_tag="VFF28_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45385.1"
                     /translation="MKKGQVTIFIILGIIILIGAGLFYVIRSESIKEDLDTEVLKAVE
                     QIPVEFRPVAAFIEGCLEDTAKEGLKKLGERGGFIDLIDNGIMTKEAPTESAAVQQAK
                     GSSLAAAYWWYLDSPNYCETDCSFGIVPEQKLALKKNPSKVSIESQLEDYIKESLQSC
                     IENFAVPRSQGFTVTEKSPPSPTVQITENDVIVLIDYPLEVERASKKELSKFYTRLPV
                     NMNRIFTIAKDITDMEAERHILERHALNLMLGFSGISDKKLPPMSDTRFSLGTPVTWR
                     RSQVKENMAKMLTSNIKLLRVYGTKNHIPYPFAAGSLFDGLYNNGMLIPGSDDYGDIE
                     VILNYNPLWDMYFDLNCNGEECRPESFMSDLVTLIGFHNYNFVYDLSFPVEVEIYDTS
                     AYNGQGYRFKYFLEANIRDNDPLTANYSGLEGAFTESTMLCDENKRTSGEVSIDTFDY
                     MTGGKLDGVQIGYSSLEETCLIGATQEGNFEGKFPVMLGGVVSFIKEGYLTESRRLDT
                     SLGIEDTLAIKLKPKLDKAFTVKKVVMAKKGIWQIENESNLTSDEQAIISLIRIPELE
                     EQPFSTSAVYWINMSEPASLQIIPGRYEMKIDLTYGKEIKIPPGKIEFEGKEYPINGT
                     TLGEGFRTGGISLNYTFTKEALAKDEIIFFVLNPDIISIPESQRSIEDVNFIAGIDET
                     ARMNRANLLPKFI"
     gene            15065..15934
                     /locus_tag="VFF28_06930"
     CDS             15065..15934
                     /locus_tag="VFF28_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45386.1"
                     /translation="MKYTIPKGKAVFMIALMVAIPIYSSMALAALSSGHVYGEDGIKG
                     LTRQGEQINMNITAAINGDSDITPSQLRLGGKSGQQFSNCIAKGSQFICSHHVDSSLL
                     DKNPYQIPIVLYNDEGELDSQYIIRGVIDKQPPEIRSFSAMPQVVSSEDLILSYDIYD
                     HSYSKDDDKCSGISRLEWQVKQLGKQPVSNVSYLNTDECHVTGSLFLSQSILNLEEGS
                     AQVVFTVYDRFGQKASATSAIEVDTTGIDIYQNSVKVTSNGKDVLFLGQQPMIVDISF
                     ITYSADLDTDRVY"
     gene            16091..>17338
                     /locus_tag="VFF28_06935"
     CDS             16091..>17338
                     /locus_tag="VFF28_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45387.1"
                     /translation="MAQNKEEFPLALPVTFDTSGPSVTSIHTNNANYAAKVSDFYVEL
                     VENDAGLLAKDIVLDLSSIRSGAVKAADACKKESSNWICVWKNIQADAADGEKTVTVS
                     GTDVLGNALTGTLSAKIMLDRTPPVIEKLEFEPLGTGGQVALPGGYYKTGDAVGINIE
                     AKEINDLRAYIDLSPYLSSGKNISIGCKNPYQDKWICSENSPGIDIPGHIVSQAKITL
                     SDAAGNTVEVKKNLEVFAYNDAKNISHWKPIVACSPSLVDRQVAEMVNTRVYCAVELL
                     PMEQDQETLLMTLGTCTENINGSLSYLEKEPELLNDEKGSTEPFLAIDLAQQKMKVDG
                     FSITCPLEIVSRIGPVITSVKEVKEIDIDLQFYNMPLGEYGEGVQKLIEDAKDDAENT
                     LWKIIGFMKLILKWAELICNALRI"
BASE COUNT         5443 a         3561 c         3661 g         4673 t
ORIGIN      
        1 gaacagcagg gcatgtatct gctgagcaag tggccaagta tattagagaa aacacaagta
       61 gctattgatg tgcttacgca cattgtgggc attcatctac cccactaacg tggggagacc
      121 tagcccacaa acttttagcc agatgaaaga aatcctagag agcataaaaa ggctaaaggc
      181 agtttgtcga tgtattactc ctgttatgga taagtgattg ttaagcctga cgattgtata
      241 tccctttgtg gatattctca aagttgaagg ccagcttgag ccattaaagc ttttttttaa
      301 agctatctgt cacgccatga gctggcgcag taatctttat atattagtac tatgaatatt
      361 acttaaatta tcaataatat ttaaaatatt tataatatta atattacaaa tgaaaaaagg
      421 ctcaggcaca aaagaaagga tttctataac tctggataaa gggctgctca aagagattga
      481 caaaaggtgt gaacagcgcc tgatgaaagt ctcaaactac atcgaaaagc tgataaaggt
      541 agggctgaaa aatgaaaagt aagaatgcgg acgcgtgcct taaaccgttc gaaggcccaa
      601 agagcagggc tttaaaccaa ccgctattga gtaagatagc catgttcatg atagcattga
      661 tactaagcat cccattctat tcagcagcag cattcgcagg cttgtcaaac gctgccataa
      721 gaagtccaaa ttcagatgat cctccaaact atataagaaa gacaggtaca aacactataa
      781 gcgtcgatgc atatatcgaa ggtgctgcag agatagaccc ttctcaggtg aggttgacac
      841 aaaaagaagg aagtccgttt gaagactgcc agcttggctt aaacggcaaa aaatggagat
      901 gcacttatcc gatagaaagc tcgtctttgc caaacggcga tttccagcgg atggttttcc
      961 ttttaaataa gagcggctcg gatgagggca gtatcaggat aaaaggcata aaagatgcag
     1021 tcgggcctga gataacctct tttactgcct cacaggcaac cattggaaaa ggcactataa
     1081 catttaatta tgatatcacc gattatatgt cagaggctga ttcaagcaac tgcattggac
     1141 tcaaagatat catatttaca gcaggcgctt acactaatcc aatagagata aactcgcaga
     1201 gcggagaatg cacctattca gacacgttca ccgaagacat cgagaagata acaaagcagt
     1261 catctggaaa tattacgata agggcgatag cgcatgacaa ctttaaccag cagtcaaaga
     1321 taaaggaatt gtctttgaag attgataggc agcagccgca gataaaagaa ggcaccttca
     1381 ggttattgga cgataacaat aaagacatcc gctttgcatc tggaacacag gcagctaaag
     1441 catcgataga agtagcagat actgatctgg ccttggtcaa agcagacttc acaagcctta
     1501 gtccaagaaa ctatgtgatg gatgcagaat gtcagcaagg tgtttgtaca tggagcggca
     1561 tctctgtaag caaaggagga actcatcagg taaagattac cgcgcaagac tcacttggca
     1621 acgaacagcc ggcaaccata ttatatcaaa tagatatcga cgatcaaaaa cctgatgtaa
     1681 agggcctaaa aagcaatcat caggattccg aaggcaaaaa ctatctgggc aaggcagaca
     1741 atcagctgat atatcagata gaagagacag gcgtgggttt tggcaaaaga aatatttacc
     1801 ttgacctaag aaacctcggc ctgggtgaaa acgtccaggc aggcaactgc acaaaaaccg
     1861 acagctcgtg gagctgctat tggaagcttc cagcattaag cgcaagtgat ggtgcaaagc
     1921 agatctttat caggccgcat tcaaccgatg atctgggaaa tgaaattaca ggtatactgg
     1981 agaatgagat tattgttgat acgattgctc caaagattgg aacgatagaa agcagcaagg
     2041 aagcagtaag gggccagcca ctgattttca cattcaccgc ctctgaccaa aatccattaa
     2101 tcgtttatgc aaacacctct aagatatctg ccgtagcttt acatactgga acatgccaag
     2161 aaagttgcag gatagagatc acagatcttg tcccaagcta tactgttcaa gagataccaa
     2221 taacagcgca ggacgtagct ggaaaccaag ccgaaaaaaa gtcaaggatt accatactgc
     2281 aggcagatgc caacacaact ccttcttttt acaggataga gcgcatagaa gtaatacctc
     2341 caaaagttga caaacaagtg atatcacaga tacctgttaa tatctttgtc catgctgagc
     2401 tagaaggcat aggctctgca gagataatca gtaagacagt agtatgtcct gccatggctg
     2461 actatctggc cggaacaagc attataggcg aagaagatac ctatataggc ttaaaactca
     2521 accaaagggc agcaactctg ccttcagtcc cgatagcctg ttcgttaagg cttttggtaa
     2581 aggataataa gaatgtttat tcaaaatatg aagaagagaa cattacagct cagcttgaac
     2641 tctatggcaa ctctcttggc gatctgacag agacaacaaa atcaaagctt gacagcataa
     2701 accaggagat aagtaataca gaggatgcta taaaaagcta tgagaaatgg ataaagacat
     2761 ggggaatctg gtgccagata tctgaaggcc ttggatttta tagctcactt gtgcaaacta
     2821 tcaagtctgc tatatggatg gtccttgctg caaaatgggc tgcatgtgca gcaggtcttg
     2881 ggtcttgcac aaaggcagtt gataaagtat ggggcgtcaa atgcacatca gcttctttgc
     2941 ctcatgctca tgttatagag aaatggatat ggccctcagg tcctataggc tttccagtcg
     3001 gcatggtaaa taaatgggct tgctccatag gctttcactg tgctgtctgc aactggaaca
     3061 catggtatga ccttggagtt tcattggttg tgcatactgc tgcaaaaaag gtgggtacat
     3121 atgatgatgg aaaaacagta agagaaatgg atagggtaac tgttgatgtg gaaggagtaa
     3181 atgacagagg aacagttgaa agtatagaag gagataatat ggtaattaaa ttggataatg
     3241 gacatactgt tacaaaacct gcatctcagg tggcaagagt aggtatcgac gacaatgtag
     3301 atatttcaat taatcgtcca gatggtagtg tagaaaaaat tcaagccaaa gtgcgcgacg
     3361 tagacctttc ctcaggagag gtcactacaa cacaaggcag ttactcacta actgatgtcc
     3421 aactttcaga tagcgtagaa caaaaaccta accagataat aggaaggcca ggtgaaaagg
     3481 tgagcgttgc atcatcacaa aacaaacgtc cttcacaaga gggcataacc actgattggt
     3541 ctattccgaa gaaaaccaac cagatcaaca gcccggtgaa tgctccaaag ggaaaggttg
     3601 aatttaagaa ttataagacg attgaagaca ctgatagcat tattattttc ccaggtgaaa
     3661 cccaagctcg ttatgcaagc actggtcagg catatactta tacaggtaaa gatgataaag
     3721 gtaacgaaat ctggcagcgc acttattcaa actatgaaga gcaaggtcag cttacaagcg
     3781 tcaccccaag cagcaactgg atatttgacc cttacaaaag catccattat gcaaaagcat
     3841 gcttatgtct gccagctatc gtatataacc ttaaaaaaga aaaacagatt caatgcatgc
     3901 aaagaaactg catatctaac cacatacaga aaggcttgcc agcaacagaa tgtgatgctg
     3961 catataaaga aaggcagtgc ctttatgttg aaggcgctga gtataagaag catgggtatg
     4021 gagcaatagc aggttctctt tggacttttg taaagaataa tcttccttat cttgcagcat
     4081 ccattggtta tcttgctaca tgttgggatt acattatttc acccgagggc atttgttcca
     4141 ctgctttggt tgtcgggact ccagcaggag gcataaaacc aacagcctgc ggcctcatag
     4201 gcagctctat gacattgatg gagatggcaa gcactatcca aagtggattt agcttcttga
     4261 actatgacca ggacttaaag ggggaggatt attgtgcgac cggaaattac cagtaaaaaa
     4321 gtgatcagga caatatttat tctcatcttg cttacagcaa tgtccaggca aagcgcttcg
     4381 ttcaatccaa ggatagacag ctggtattct gtagagcagt gggaggttga cctttgctca
     4441 aagtggggag gcacctatga ggctcagtca gaagctacgt caagcactgg catctccttg
     4501 tcacagacaa cactatctct gcagggaaga aagcaagatt ataatataaa aggagtaaat
     4561 gaaagcctct atactcttag ctggtatctt gagcccttgg cagagatgag ttacactatt
     4621 gaattgacag acgacgatga gataaaaaca tacaggattg attcaggtca ggcctctttt
     4681 gcaaaacctg caggtggtta taaatcagaa tattatagtg aggattatac ttacctaaag
     4741 atgacttatg gcacaaactg gttgagggtt ccgctggttc aatgaaaaaa atatttcaag
     4801 tgctggctct aaacatcgct tatctaatta tattgctcgc aataataagc ctgttttctt
     4861 ttgcagtaag agaaaatctt agctccatgg caaatataag cgatccatta gcaaagatta
     4921 gtcagatgat ggccggggca atagagcctg attctatcat ccttaaggat atggcagccg
     4981 tatcttcttc catccgatgg ttctttgcca cgatgatagc agtggcaata ctgactataa
     5041 tagcagcttg ccttaccgca gggtacttta aggcctggat ttggtctaag attacaagca
     5101 taccattgaa aaaatatgca gcgttaaaac tttcattcct tcttggcctc tggacgctct
     5161 cttggctgct tgtatcgata tttctggttt ttacgctaaa gttttcaaga gatgttctaa
     5221 tcaagataaa ccttatctat ttcattttat ttgcctattt cagtctgctt ctcattcctg
     5281 tttttataag aaagcagcag attattggct cgcttaagga aacgtttagg ctcggcacaa
     5341 aaaaattcta tgaattcata cctgcctttg ctttgatagg tctggtagcg attttgtcct
     5401 ttgcaactgc aatctatctt gcaatgatat atagtccatt aggcctgatt gagataccat
     5461 tgtttttatt ttttatcgta tggactaaat atttaatcaa taataaattt acgagggtat
     5521 gcagatgaaa aaagcccaga taactgtatt tatcataatg ggattcttga ttttgcttgc
     5581 tgggatagcc atcttttact tcacaaggga atctgtaaaa agcgaccttt catccgaggt
     5641 agaaaaagcc caggatctgc cgctatgggc agccgaggta aagccttatg ttgataagtg
     5701 catacaggac ctctcaataa aagcatttaa taagattgga gagcatggag gatatatcga
     5761 tctaaacgat agctacctta caccttctgt tctctccctc aatccctctg acccgacaga
     5821 agctgatgca gtggcaatct ctgataaacc tgcctcatcc attgcatact ggtggtacat
     5881 gcagacgcaa aacacctgtg atgactgcat cgtatcatcc aatgacccgc ctatagatat
     5941 gattgaatgg caggtagcaa gatatgttga taggcagctg ccagattgct tgcagaattt
     6001 tacagtatta aatggcattt cggtaaaaag aggcagcata aactcaacag tcacaataaa
     6061 tgaaaaagat gttgatgtac aggttctaat gcccacgcag ataagtaatg tagacatgtc
     6121 agcctcaatt gaaaagtata agataacctt gccgctggac tttaaccatg cctatcttgc
     6181 agctgcactt atcgcaaatc atgagatatc tgaaagattt ttagagggca ttgtaaatca
     6241 cataatcacc atctcatcct ttcaggatca agacaaacta ccccctattt ctgcgataga
     6301 cagtaagaat gggagaacca catggagaaa aagcgaagta aaaagaagga ttgtcgatga
     6361 tgtcttgctg tcaaggattc ctgcgcttca gataaaaaat actaagacat atactcagct
     6421 ctcctcccaa gatccactta cccaaggctt aaatgatatt atggcattag atattctgcc
     6481 tgaaggatat gaaaatcttg aggtaagctt tatctacaat cctgattgga acatattctt
     6541 cgacataacg ccaagctcag gcgaggttct tggtccattg acaacaagga cagatgacac
     6601 aggtataggt ttctttcctc aatcagtgaa caacttctat gaattctatt atgacctttc
     6661 ttttcctgtg cttgttatga taaaagatcc agactcactg aaagaccagc aaggctacca
     6721 atttatcttt gctctcgagg caaatatccg agacaacaag gatttatacc aatggaatca
     6781 tggaaatggg acaataggcc aggcagatta tcgtcgagtc tctttggcag caaaaagcag
     6841 ccaggtcgat gcgaaggctt gcgagcaaga aggctcaaag tggcgctgcc ctcttgacga
     6901 tcaggtttat agcgatatgc ctaagtgtat cgacaaatgt aaaacatcca ctcaacagac
     6961 aaaagacatg acccttcttc caagcttgtt ctgtgatagt aaccaaagga taagcggcaa
     7021 tataacagta aatgcctatg atgcaaagac gcttctgcct ttgccaggcg ttcaattgac
     7081 atttggatgc ggcaattaca ggaaatgtcc tatggaaggg actgatagcc agggaaccta
     7141 cataagcagg tttcctgttt gcatcgggga tggatatatc cgtgcagaaa aagaagggta
     7201 tatgccagaa tacgaagggg gtgtttcaat aagccctgaa accaagaccc gttatgatat
     7261 ataccttgaa cccatcagga caaaaaaaat agaagcagcc tacataaata ttaccaacat
     7321 ctataggata aaaaatacgc ttcatccaaa aacaggagaa agcatgctgg atgagctgta
     7381 ttataagaaa tttgagactg cgcagggagc tcagattacc atccaccaag gtatcctggg
     7441 agaaatatac tataaggttg atgacaaaga aaaacgaacg gttttgttga aggcaagagc
     7501 tattgaaaac gcacgagatt tattgaaaga tattgaatac catcaagaag caaaaaaaga
     7561 gccggttgtg gatgtcttct tttcagtgaa agaggctgca gaatttgccg gagcaagatc
     7621 tgatctttta tcctttgtca gtaagagcaa ggttgattcc tatattgatt ggttcaagaa
     7681 agaagagaat aatatccaat tccgttctga tacttacttt tcctctgtta taggcatggc
     7741 aaggtcacag gccaatagct tgaaggacaa gcaagttttg accataacct atcagaaggc
     7801 aaaggaaaag ccatatgagc aaaacatgcc ttcatcaatt gctcagctgg gaaactttaa
     7861 tacgactatt agtctggtcc cgggaatcta taacttaaac atgatgttta gggatgatca
     7921 ggggtatgtt attccggcaa aagacggtta ttctgagttc aattatactc ctgcaatctt
     7981 agggggggct cagctggatg aaaagacatc ccaatgggtc atagacaaag aaagacttga
     8041 tgctagttca acaataaagt tctatttctt taaaatagat aatccaactt cgcttgagga
     8101 cacaggtgag ataggctcaa tagtgaatta ttcgatagat tatcgcccct acatcgagcc
     8161 agaattcctg ccttaagcct gctcatcttc tagtagccat ttccacacag gctttacgat
     8221 gatttttttg ctgcctgaag atatctcctt ctcggtatcg tcagtaagga ttaatccttc
     8281 tctcaacctg tacttttcca tggcttcaag caggccattt acctccctgt cagcctcaag
     8341 actataacag acttggatcg cttgcgatat tctcgggccg tctctcatga taaaatcaca
     8401 ttcatactta tccctatgat aaaagatttc cttgcctttt cttttaagct cgataaagac
     8461 tatattctca agtatccttc ccttatcttc gcttggccta aagccaagca gcttgaccat
     8521 gccattgtcc acaagatata ccttcttgtt ggaatatatc tgattattta gcgaagcgct
     8581 gtacctaggt ataagaaagg caaggtagct gttctccaga tactcaaagt attcccttat
     8641 ggttgttgcg cttgtcaggc ctgcaagctt tctaatgctg ttaaaactta tctcttttcc
     8701 aatattgctt gctgcataat atacagtctc ctttagtgaa cgttcagtgg gaagcttgta
     8761 tctggatacg atatctctat agagtatgtt ttcatatact gattttatat actcctcttt
     8821 ctctgtttga agatattcag gaaatcctcc tttctcaaaa aactcgttga atagtttttt
     8881 tatctggcct tttttctctg atgtaagatt atctaaggac ggctcgaaat ccttgaacag
     8941 caaaaattcc ctaaatgaaa aaggatataa tgaaaatgag atgtttcgcc cagtcagatg
     9001 tgttccaagg tctttgctaa gcatgcttgc atttgagcca gtgataaaca ccttgctgcc
     9061 gttgtcattc aatctcctca caaatcgttc ccatgaattt atgttttgga tttcatcaaa
     9121 aaagaaggta ttttttttgc caaaaagctc aataagaagc tcatacatag tctgaaagtc
     9181 atcgacgtga aagtcaataa gcctgtcgtc atcgaaatta acataatagc tttctcttct
     9241 ttttgccctc aactcatgta gaagggtaga ctttccagcc cttcgtatgc ctgaaactat
     9301 tataataaag ggggtctttt caaaagtatc tatctttctg ataagctctc taggtaccag
     9361 tttcccatct ctaaatttat tctgctcggt taaaacctgc cttagttttt ctttttccat
     9421 attattagag taaaggttag ttatttataa agctttggtt tgacaaacca aatttaagcc
     9481 atattttatg gtttaccaga ccaaatttac agaagttccc ccaaccatga atgagagatt
     9541 ctcagctcgg aattctagca attcagatgt gtattcgccc ctgaccatga atgtgattct
     9601 gccgtccaat ttaaatgctt gtctcactca tcatcttcaa gctctcacag ctctgcttga
     9661 gatacatgtt ggtaagcttc tcgcacacag aataatcccc ttttgtggca aaatccatat
     9721 agcactggtc tctcctgctg tctttaacca tctttgcgca gatcgtgctg tcttcgtttt
     9781 gtactgccac ttcctttatg cacgaatccc ttgaaggctc atcttctata ttatcacata
     9841 ctgattcgtc tcttgtgcct gcagcgacct ttgtcatgca gtcaaatctg aaggacgcct
     9901 gctctatctt cttgcagtct gctattgccc ggggcttatc attctttgct atgtttccta
     9961 ttatatctat ctgctcccag acaggcctgt cgccaaatga gtcctgaggc agatcagaag
    10021 cacagtcctc aaggcagctg ttggaatctt caccgttctc gcaaagaccg tttccgcagc
    10081 actgtgcaac cttgctatgc tcgcattgat agcctgtatc agcgctgcaa tagtcagtgg
    10141 tgcatttgtt cttgtcctcg cagcttgatg ggcagcttct ttttggcaca tcaggctgtt
    10201 gtcgaataga aggctcggca gcagcctgta tcctgaatgc gtttgtcgct cttgctttct
    10261 tcccaccgta gcttgcaaca gctcttacat aatagtttcc tggctttatg tcgccgacac
    10321 cgatattgac cgttgatgac gcccttgtct cgacagcaag cgtctcttct ttgaaagcaa
    10381 gcaccctttc agtattgtca tagacttcat acttcatgaa tacatcaaac ctcttgctct
    10441 tgcccaggtt gaatatctgt gcagtaaact taaggctctc tccttggttg accgtgcttg
    10501 tgatgacatc catctgcaca tcaaggagct gcatgtcagg tttttctttt atcacaaaca
    10561 tgtaaacccc agcacccgat aagacaaggg tgcatattat ggcgattatc aaagctatcg
    10621 tccgcttcga gaaggttatg acatgcttga cttcggtcgg cggataggca aactggaaag
    10681 cttcatttat ctgggattca gaatacccat atttggtaag atagcctttt atctctcctg
    10741 cagaataccc tgcccttatc tgctccctta tatatttgac aaggctattt tgcaccatct
    10801 agctcctctt cgatgaattt ctctatcttg gcagacatgt ttatgcagtt ctctttgcag
    10861 tattccctaa agcgaaagac aagcttgctg tctattgtaa aattgcaccg ttcttttgtt
    10921 attttcttgg acatacacac ctatacatat agatatatat gcatgtgtat ataaagcttg
    10981 cgcatataat tctaacacta aatagtagtt acaaaggcat aggtttataa ggaagggcta
    11041 accttcttta gttatgagaa acgtggtagt gatttatgga cctaatgaaa aactaggaat
    11101 ggcagtagca gattctttta gagatagatc cgatttaact aatctatttt tttatgcagc
    11161 tcagaactca agacaactat ctgatattga aatggcgaat ataacatcaa ccgcaaggaa
    11221 actcggcgat tatgttgtcg ctgatcttca actcttaatc ctaaacgccc atggtgcagt
    11281 taagccagga aaagccccac taaacgtagg aacatttggg tatgatatat ctccttctag
    11341 gggggtttat ccaactgaac tatatgtcaa tgaaacccct tttggggacc agccttatat
    11401 gcggataagt gcaatagtat caagttctga tttaaatggc aaagagattc aaagaaaagc
    11461 acgtcttatt catggattgc tagaccgagt tgcccgacag gaaaaaggcg acgtcgtagc
    11521 agcattacaa agaaggaaga aagaagcagc ataggccaat atgaatctaa gcgcctattt
    11581 atttgcatac tatttttttc aatttttctt tgagggcagg aagatcctct gttacaatga
    11641 tccatgttag gttcaagtca attgcaaaat aagcatggat aagtatgtcc cttgtccctg
    11701 caatctgtca agaacgccag tgataggcaa gggaaatgct aaggtaggac acacagtaag
    11761 gcggttcttg agcatgctca gaaattttgt tcttgcaatg aagcccttgc tttgaggcat
    11821 ccatcccaga ctggatggaa aatttctgac atttttccat tcgatatccg gatatctctc
    11881 tttaagctgg gatgagatat gtttcacagc ttcaccgata atctctatcc tgcgtaaggt
    11941 ggcatcctga atgtctacat cctctctgaa ttgctgctgg gatattctat ttagggttcg
    12001 ctcaatcttg ccaatctcct gcaggatatg ttcgataaac accttatcct tattcatagg
    12061 actttcctct catcttttaa tatattttgc cgaagcaatg gatggattga atcataggtt
    12121 aatacatcga ttttttttaa gtttctcttc caattctatt ttcagcccag caaggtcaag
    12181 caggcttttt ttcccctgaa ttttatcaat aggtctatat cactgctctt tgtcgcctcc
    12241 cctcttgcat aagagccaaa gattcctgct ttaacaacat cattcttcct taagatggct
    12301 actatggttt ttctcatcct tgtatccata gtcattccca taataataga gttagatgcg
    12361 gtgttttaat aatcttttga taacgaaagc atggtaggca aatatctatt gacagatcat
    12421 tcctaaattc cctcatattc aaagattagt taagcgccat atatctattt accccccaaa
    12481 atacccgttt atggcaagat ctttcctatg aataggttat ttcctttcaa aatcgaggat
    12541 agaaaatttg cccgtataag gcttgaaact ccacctctta catgattaag agttatgaac
    12601 ttttcattca accatttctg gagaggggtt caacgctttt aaacctgctc atagcatagc
    12661 caaagcacaa ataaatctac ttcgtttatc cctagtttag cctaaatcaa gcatatagct
    12721 aggctattta gcctatattt agcctaaatt aagtacaagc tatggcttaa taaaagcaaa
    12781 aggtttaaat acgagcaatc aaaaaagtag caatagataa tgagccaaca gataaggata
    12841 acggttagcg acgagataga taggcttctt tctaaggtag caaagcgtct gggaaaaaag
    12901 aaatccatgc ttgcaagaga actcatggaa cagaagctct atgacctaaa ccttatacaa
    12961 agaggctttg aggacctaaa atgaaaaagg gccaggtgac tatattcatc atccttggaa
    13021 taatcatcct aataggcgca ggattgttct atgtcatcag gagtgaatcg ataaaagaag
    13081 acctggacac agaggttcta aaggcagtcg agcagattcc tgttgagttt aggcctgtcg
    13141 ccgcattcat agaaggctgc cttgaggaca cagcaaaaga aggcctaaaa aaactgggag
    13201 aaagaggcgg cttcatcgat cttatagata atggaataat gacaaaggaa gctccgactg
    13261 aatcagcagc tgttcagcag gcaaaaggct catctcttgc agcagcttac tggtggtatc
    13321 ttgattcccc aaactactgc gagacagact gcagctttgg gatagtgcca gaacaaaagc
    13381 ttgcgctaaa aaagaatcct tcgaaagtct ctatcgaatc ccagctggag gattacatca
    13441 aggaaagcct ccaatcatgc atagaaaact tcgcagtccc aagatcgcaa ggtttcacag
    13501 ttactgaaaa aagcccccca tcaccaacag tacagataac cgagaatgat gttatcgtct
    13561 tgatagatta tccgcttgag gtagaaaggg caagtaaaaa agagctgtcc aagttttaca
    13621 caagattgcc tgtcaatatg aacaggatat ttactatagc aaaagatatc accgatatgg
    13681 aggcagagcg ccatatactg gagcgccatg cactcaacct tatgcttgga ttttcaggga
    13741 tctcagataa gaaactgcca ccgatgtctg acacaaggtt cagccttgga acgcctgtta
    13801 catggagaag gtcgcaggta aaggagaata tggcaaagat gctcacttca aacatcaagc
    13861 tcctcagggt ttatggcact aaaaaccaca tcccctaccc atttgcagca ggctctcttt
    13921 ttgacggtct ttacaacaat ggcatgctga tccccggctc agatgattat ggcgacatag
    13981 aggtcatact caattataat ccattgtggg atatgtactt tgaccttaac tgcaatggag
    14041 aagagtgcag gcctgaatcg ttcatgagtg atcttgtcac actgatagga ttccataact
    14101 ataactttgt ctatgaccta agttttccag tagaagtaga gatctatgac acctcagcat
    14161 ataatggaca aggctatagg ttcaagtatt tccttgaagc aaatataagg gacaatgacc
    14221 cgctgacagc aaattactca gggctggagg gagcatttac ggaaagcacc atgctctgtg
    14281 atgagaacaa aaggacatca ggagaggtct ccatagatac cttcgattat atgacaggcg
    14341 gcaagttaga cggcgttcag ataggatact caagtttgga agagacatgc cttattggcg
    14401 caacgcaaga gggcaatttt gaaggaaagt tccctgtaat gcttggaggc gttgtaagtt
    14461 tcatcaaaga aggctatcta actgaatcaa gaaggcttga tacttctctg ggcatagaag
    14521 ataccctggc aataaagctc aagccaaagc ttgacaaggc attcacagta aaaaaagtgg
    14581 tcatggcaaa aaaaggtata tggcagatag aaaacgaatc aaacctcact tcagacgagc
    14641 aggcaataat ctccctcatc aggatacccg agcttgaaga gcagccgttt tcgacaagtg
    14701 cagtttattg gataaatatg tctgagccag caagcctcca gataatcccg ggcaggtatg
    14761 agatgaagat agacctgacg tatggcaagg agataaagat acctccagga aaaatagagt
    14821 ttgaaggaaa agagtatcct atcaacggca ctacattagg agaaggattt aggacaggcg
    14881 gaattagcct aaactatacc ttcacaaaag aagctctggc aaaagatgag ataatattct
    14941 ttgtgctaaa ccctgatatt atatcaatac ctgaatcgca aagaagtata gaagatgtta
    15001 attttatagc tggtatagat gagacagcaa ggatgaacag ggcaaacctg ctcccaaaat
    15061 ttatatgaaa tacacaatac ccaaaggaaa ggcagtattt atgatagctt tgatggtagc
    15121 tatccctata tattcatcta tggctcttgc tgcactaagc agcggccatg tctatggaga
    15181 agatggcatc aaaggactga caaggcaggg cgagcagata aatatgaaca taaccgccgc
    15241 tataaatggt gactctgaca tcacaccatc tcagctaagg ctgggtggca agtctggcca
    15301 gcagttctca aactgcatag caaaaggcag ccagtttatc tgcagccatc atgtggactc
    15361 aagcctgctt gacaaaaatc cataccaaat accaatagtg ctctacaatg atgaaggaga
    15421 gttagacagc caatatataa taagaggagt tatagacaaa cagcctcctg agataaggtc
    15481 attttcagcc atgccccaag tggtcagtag tgaagacctc atccttagtt atgatatata
    15541 tgaccattct tacagcaagg acgacgacaa atgcagcggt ataagcaggc tcgagtggca
    15601 ggtaaagcag ctgggtaagc agccagtgtc aaatgtttca tacctaaata ccgacgagtg
    15661 ccatgttaca ggctctcttt tcctctccca gagcatttta aatctcgagg aaggaagcgc
    15721 ccaagtagta tttactgttt atgacaggtt tgggcaaaaa gcatctgcca cgtctgccat
    15781 agaggtagac acgacaggca tcgatatata tcaaaattca gtcaaggtta caagcaatgg
    15841 gaaagatgtc ttgtttttag gccagcagcc gatgatagtt gatattagct tcatcaccta
    15901 ctcagctgac ttggacacag acagggtcta ttgagacttt tcagccctaa atagcggcaa
    15961 tatccccagc tataagaata agaaagcttc ctgctcgcaa tatgaagaag gcttcaggtg
    16021 tatatttgat ggtatagagg ttaagctttc ttcaacatcc acgccggcga taaggataac
    16081 agcagaagac ttggcgcaaa ataaagaaga gttcccgctt gctcttccag ttacatttga
    16141 tacatccggc ccaagcgtaa catccatcca tacaaataac gcaaattatg cagcaaaggt
    16201 ctctgacttt tatgtagagc ttgttgaaaa cgacgcaggc ttgcttgcaa aagacattgt
    16261 cctagaccta agcagcataa ggtcaggtgc agtaaaagca gcagatgcat gcaaaaaaga
    16321 aagttctaat tggatttgtg tatggaagaa tatccaggca gatgctgccg atggtgaaaa
    16381 aacagttact gtgtcaggca ccgatgttct gggaaatgcg ctgacaggaa cactgtctgc
    16441 aaagattatg ctcgacagga ccccgccggt gatagaaaaa ctggagtttg aacccctagg
    16501 cactggaggc caggttgctc tgccaggagg atactataag acaggagacg cagtaggcat
    16561 caatattgaa gcaaaagaga taaacgatct tagagcatac atcgatctca gcccttacct
    16621 ctcttctgga aaaaacataa gcataggctg caaaaatccc tatcaggata agtggatatg
    16681 cagcgagaac tcaccaggca ttgacatccc aggccatatc gtgagccagg caaagataac
    16741 attatctgac gctgcaggaa acactgttga agtcaagaaa aaccttgagg tgtttgccta
    16801 taatgatgca aagaatattt ctcattggaa acccattgtt gcctgctctc cttcacttgt
    16861 tgacaggcag gtagcagaga tggtaaatac aagggtgtat tgcgcagtag agcttttgcc
    16921 catggagcag gaccaggaga ctctgcttat gacccttggc acatgcacag aaaatatcaa
    16981 tggctctcta agttatcttg aaaaagagcc agagctgctc aacgatgaaa aaggaagcac
    17041 agagcccttc cttgcaatag accttgctca gcaaaagatg aaggtagatg gctttagcat
    17101 cacctgcccg ctggagatag tttcaaggat aggccctgtc ataacaagcg tcaaggaagt
    17161 aaaggagata gacatagatc tgcagtttta taacatgccg ctgggcgagt atggagaagg
    17221 cgtccaaaag ctcatagagg atgcaaaaga tgacgcagaa aacaccctct ggaaaatcat
    17281 aggtttcatg aagcttatcc taaagtgggc agagcttatc tgcaatgcgc taaggata
//
LOCUS       DASRUK010000158         5000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1962189, whole genome shotgun sequence.
ACCESSION   DASRUK010000158 DASRUK010000000
VERSION     DASRUK010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1962189"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VFF28_06940"
     CDS             <1..185
                     /locus_tag="VFF28_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45388.1"
                     /translation="RFDNITATRRIPFIVLSPSERLPDFSERYAFDRQIGSFKIFRAI
                     APGQNVFDQSRGQAEI"
     gene            197..904
                     /locus_tag="VFF28_06945"
     CDS             197..904
                     /locus_tag="VFF28_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZX45389.1"
                     /translation="MYKNKKVSVVFSTYNEKDSVRGCIEGLFRTGYVDEVIAVDNNAV
                     AGTKDEIKKTKASYFHEKHQGFGYGFQRALRESTGDILIMIEPDGTFDPQDIVHLLRC
                     SDSFDVVFGTRTNSRAASGKAHMSFLLRQGNYALAKLVSILFRAPPLTDVGCTYRLIK
                     RKAYDELRERFTVMGDIFNLDMMLHVVKNKIPYTEVSIRYLKRIGVSSLSGNFLRSIS
                     IGFAMFFLILKFRLGFR"
     gene            933..1814
                     /locus_tag="VFF28_06950"
     CDS             933..1814
                     /locus_tag="VFF28_06950"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HZX45390.1"
                     /translation="MGIDALRKGRIKSIIQLTRPHQWYKNTVIFLAILFSRNFFNIGY
                     LAVSVLGFLVLILVSSANYIFNDLIDLRRDRLNPEKKGRPLVSGEVDIYTAIFLLVIL
                     SSVGLIGAYLLDIYFFFAVLAISITSTVYTVYLKDILFADLLAISLIFGLRAVSGAVI
                     IHVFVSPWLLVGVFFFSLFLVTGKRYGEAEFLEKRSASHRAVLRYYSKTLLYSLLNIF
                     MTVLIMVFTLYSFFSDYKSLIWTTPLFIYLILRYYYLILSNSEIVRNSEKAFKDYYLL
                     IPMVIFMIAGFLIVFLS"
     gene            complement(1826..2776)
                     /gene="rfbB"
                     /locus_tag="VFF28_06955"
     CDS             complement(1826..2776)
                     /gene="rfbB"
                     /locus_tag="VFF28_06955"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HZX45391.1"
                     /translation="MAKVLITGGAGFIGSNFVKHYLKVHPDDEIINLDKLTYAGNLNN
                     IKELEGNKNHTFIRGDICEKKVVEKAMEGCSKVIHFAAETHVDRSIIDAGTFIKTDVF
                     GTYRLLEAARKLNVKRFIHISTDEVYGSIEEGSFNEKSPLNPRNPYSASKAGADRLAY
                     SYFTTYGLPVTITRSSNNFGPYQYPEKLIPLFITNLLNGKRIPLYGDGLNVRDWLYVQ
                     DNCEAIEFALEKGKPGEAYNIGGGNEKPNIWITKFLLKELGKPESYIDHVQDRLGHDR
                     RYSLDTTKIKELGWKPRFKFENALRETVRWYQDNPCWWQG"
     gene            complement(2778..3239)
                     /locus_tag="VFF28_06960"
     CDS             complement(2778..3239)
                     /locus_tag="VFF28_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HZX45392.1"
                     /translation="MIEGVHIKKLKVIPDERGRVMEMLRDDDEVFKKFGQVYLATAKP
                     GYVKGWHYHKKQTDNIICVKGEIRMGLYDPRKESKTFGKVHEFIISAERNPLLISIPP
                     EVYHGFESNVNEESMIINIPTEHYCHDGPDEHRVAFNSKDIPFQWRSTKGG"
     gene            complement(3236..3964)
                     /locus_tag="VFF28_06965"
     CDS             complement(3236..3964)
                     /locus_tag="VFF28_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZX45393.1"
                     /translation="MKGVILAGGTGSRLMPLTKITNKHLLPVWKKLMIYYPLEKFRDA
                     GIKEVLIVTGKEHAGDFIKILGNGKEFGMSLTYGLQEEAGGIAQALSLANNFLKYENK
                     FAVILGDNILEDDIRKDVADFEKGKEEAHIFLKKVKDARRFGVAELKGDEVVGIEEKP
                     EKPKTDYAVTGLYLYSNNVFEIIRTLKPSKRGELEISDVNNDYIQKGTIKATILSGFW
                     TDAGTFESLFRATKLLRDKEERDK"
     gene            complement(3961..4827)
                     /gene="rfbD"
                     /locus_tag="VFF28_06970"
     CDS             complement(3961..4827)
                     /gene="rfbD"
                     /locus_tag="VFF28_06970"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HZX45394.1"
                     /translation="MKVLIVGASGFLGKKLYSSLSKEFMVIGTSQKSNGNLIKMDITE
                     RKEAAELIISIRPDIVIVSAAMTDADLCEIEKERANNINVIGIKNITIACVKINAKLI
                     FFSSDYVFDGKKGGYNEDEDTNPLNFYGKTKVESEEIVKNSGLSYIIARISWLYGFNS
                     PGDKQTFVNFVVNNLKSGKEIRIVKDQVSNPTLIDDIADAIKALIKKKEEGLFHIAGR
                     EPISRYDFSLRIAKVFGLDTNLIKETTTPLLRQKASRPANSSLDITKLQSLGVRMSNV
                     YEGLKKMKRQMV"
BASE COUNT         1264 a         1088 c          974 g         1674 t
ORIGIN      
        1 ataggtttga taatataaca gctacccgca ggattccatt tattgtattg agcccttctg
       61 aaaggttgcc tgatttttca gagagatatg cctttgacag gcaaatcgga agttttaaga
      121 tatttagggc gattgcacca gggcaaaatg tatttgatca aagccgtggc caggctgaga
      181 tttaaggtat cacatcatgt acaaaaataa aaaggttagt gttgtatttt ccacttacaa
      241 cgagaaggac tctgttagag gatgcattga gggattattt aggacagggt atgtggatga
      301 agtcatcgct gtagataata atgctgtggc aggaaccaaa gatgagataa aaaagaccaa
      361 ggctagctat tttcatgaga aacatcaggg gtttggctat ggatttcaaa gagccttgag
      421 ggaatcgacc ggtgacatac tgatcatgat tgagccggat gggacatttg atccgcagga
      481 tattgttcac ctgttgaggt gttcagatag tttcgatgtg gtctttggaa ccaggacaaa
      541 ctcaagggcg gcaagtggta aggcacatat gagttttctg ctcaggcaag gaaattatgc
      601 tcttgcaaaa cttgtaagca tattgttcag ggcaccgcca ttgactgatg taggatgcac
      661 ttataggctt atcaaacgaa aagcatatga tgagctaagg gaaagattta ccgtcatggg
      721 agatatattt aacctggata tgatgctcca tgttgtaaaa aacaagatac cttacacaga
      781 ggtttcgatc cgctatctaa aaaggattgg agtctcatcc ctatcaggca attttttaag
      841 atcgataagt ataggatttg ccatgttttt tctgatcctt aagttccgat taggcttcag
      901 atgaaaggca tgcatgcgga tatacaagtt agatggggat tgatgcattg agaaagggac
      961 gaattaaaag cattatacaa ttgaccaggc ctcatcaatg gtacaagaat acggtcatat
     1021 tccttgctat ccttttctca agaaattttt tcaatattgg ctatcttgca gttagcgttc
     1081 ttggattcct ggtgcttatt ttggtgtctt cggctaacta tatcttcaat gatctgattg
     1141 acctaagaag agacaggcta aatcctgaga aaaaaggcag gcctcttgtg tcaggagagg
     1201 ttgatatata tactgcgatc tttcttttgg ttatattgtc gtcagtgggc ttgattggtg
     1261 cttacttatt ggatatttat ttcttttttg ctgttcttgc aatatcgata acctcgacgg
     1321 tatataccgt gtatttgaag gatatactct ttgcagatct tcttgccatc tccctgattt
     1381 tcgggctcag ggctgtctct ggagcagtga taatacatgt atttgtcagt ccctggctcc
     1441 tggttggcgt ttttttcttt tccctttttt tggtaacggg caagaggtat ggtgaggctg
     1501 aatttcttga aaagagatca gcaagccata gggcagtact aagatattat tcaaagactc
     1561 ttctctattc gcttctgaac atctttatga cagtattgat aatggtattt acattatact
     1621 catttttttc agattataag tcccttatat ggacaactcc tctgttcatc tatcttatcc
     1681 tgagatatta ttatctgatc ctttcaaata gtgagatagt cagaaattca gagaaagctt
     1741 ttaaagacta ttatctgctt ataccaatgg tgatatttat gatagctgga tttcttatcg
     1801 tatttctttc atagaactag tagcattatc cctgccacca acaaggattg tcctgatacc
     1861 atcggactgt ctctcttaag gcattttcaa acttgaatct cggcttccag ccaagctctt
     1921 ttattttggt agtgtcaagt gaatatcttc tgtcatgccc tagtctatcc tgtacatgat
     1981 caatatatga ttctggcttg cccagttcct ttaggaggaa cttggtgatc cagatgtttg
     2041 gtttttcatt ccctccccct atgttatatg cttccccggg cttgcctttc tcaagggcaa
     2101 attctattgc ctcgcagttg tcctggacgt ataaccagtc tctaacattg agcccatccc
     2161 catacaacgg aatccttttg ccattcagta gattggtgat aaacaaaggg atcagctttt
     2221 caggatattg gtatgggccg aaattgtttg aagaccgggt tattgttacc ggcaagccat
     2281 aggtggtgaa gtaagaatag gcaagcctgt ctgcgccggc tttgcttgcg gaataggggt
     2341 ttctgggatt taagggtgat ttttcgttga acgatccctc ttctatgctc ccatatactt
     2401 catctgtaga tatatggata aatcttttga cattaagctt ccttgcagct tctagcagcc
     2461 tgtatgtccc aaatacatcg gtcttgataa aggtcccagc gtcgatgatg cttctgtcga
     2521 catgcgtttc tgcagcaaaa tgaataacct ttgagcagcc ttccatcgct ttttccacga
     2581 cctttttttc gcagatatcg ccccttatga aagtatggtt cttgttgcct tccagttcct
     2641 tgatgttgtt aagattccct gcatatgtca gcttatcaag gttgatgatc tcatcatccg
     2701 ggtgcacctt taggtaatgc ttcacaaaat tggaaccgat aaagccagct ccgcctgtga
     2761 tcagaacttt cgcgatttta gccacccttt gtcgatctcc attgaaaagg gatgtccttg
     2821 ctgttgaaag ccaccctgtg ctcatcagga ccatcatggc aataatgttc agtcggtatg
     2881 ttgattatca ttgattcttc attcacattg ctctcaaatc catggtatac ctcagggggg
     2941 atagagatca acagggggtt gcgctctgcg ctgattataa attcatgcac ttttccgaag
     3001 gtcttcgatt ccttcctcgg atcatatagc cccatcctga tctcaccttt tacacaaatg
     3061 atattatcag tctgcttttt atggtagtgc cagcctttga catagccggg ctttgctgtt
     3121 gccaggtaga cctgcccaaa tttcttgaaa acctcatcat catccctcag catctccata
     3181 actctgcccc tctcatcagg gatgaccttt aatttcttga tatggactcc ttcgatcatt
     3241 tatctctttc ctccttatct ctcaataatt tcgtcgcccg aaaaaggctt tcaaacgttc
     3301 cggcgtctgt ccaaaaaccg cttaggattg tggccttgat tgtccctttc tgtatataat
     3361 cgttatttac atcgcttatc tccagttccc cgcgctttga aggcttcaat gtccttatga
     3421 tctcaaaaac gttatttgag tacaggtata aaccagtcac agcatagtct gtcttcggct
     3481 tctcaggctt ttcctcaata ccgactacct cgtccccttt aagttcagca acgccaaaac
     3541 gcctggcatc cttgaccttt tttaagaaga tatgcgcctc ttccttcccc ttctcaaaat
     3601 cagccacatc cttcctgata tcatcctcaa ggatattatc tccaaggatg actgcgaatt
     3661 tattctcata cttaagaaaa ttattagcaa gcgagagcgc ctgtgctata cctcctgcct
     3721 cttcctggag cccataggta agtgacatgc cgaattcctt gccgtttcca agtatcttga
     3781 taaaatcccc tgcatgttct ttccctgtta ctataagcac ctctttgatg ccagcatccc
     3841 tgaatttctc aagcggatag tatatcataa gctttttcca cacagggagc aggtgcttgt
     3901 tggttatctt tgtcaagggc atcagcctgc tgcctgtccc tcctgccaag attactcctt
     3961 tcataccatc tgccttttca ttttttttag cccttcatag acatttgaca ttcttacacc
     4021 aaggctctgc aattttgtta tgtctaagct tgagtttgca ggcctgctcg ccttctgcct
     4081 taacaggggt gtggttgtct cttttattaa atttgtgtcc aaaccgaaaa ccttggcgat
     4141 ccttaatgaa aaatcatatc tggagatagg ctctcttcct gcgatatgga ataagccttc
     4201 ctctttcttt ttgatcaagg cttttatcgc atcagcgata tcatcgatca atgtgggatt
     4261 tgatacctga tcttttacta tccttatctc tttgccgctc ttcaggttat tgacgacaaa
     4321 gttgacaaag gtctgcttgt ctccaggtga attaaatcca tacagccagc ttattcttgc
     4381 gattatatat gaaaggccgc tgttttttac tatctcttcc gattcaacct ttgtcttccc
     4441 ataaaaattt aatggatttg tatcctcgtc ttcattatat cctccctttt tgccgtcaaa
     4501 aacatagtcg cttgagaaaa atattagttt tgcatttatc ttgacacagg caattgtgat
     4561 attctttatg ccgatgacat ttatattatt tgccctctct ttttcgatct cgcaaaggtc
     4621 tgcatccgtc atcgctgcgg aaacgattac gatatctggt cttatggata ttatcagctc
     4681 agccgcttct ttcctttctg tgatgtccat cttaatgaga ttgccgttgc tcttctggct
     4741 ggttccgatg accataaact ccttggataa agatgaatat aattttttgc ccaagaaccc
     4801 actagcccca actatcagaa ctttcacttt ggcttgacaa aacagtaaat tatttaaata
     4861 cttttctaaa gctaagctgt atctaataat atacctgttc tataacatct ctgaaatgcg
     4921 acagctaaac tatacatttt tctcaaacct gtcccaataa tctgtgattg tgccataggt
     4981 cactccttct ttgcgaatct
//
LOCUS       DASRUK010000159         2470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_470907, whole genome shotgun sequence.
ACCESSION   DASRUK010000159 DASRUK010000000
VERSION     DASRUK010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470907"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VFF28_06975"
     CDS             <1..769
                     /locus_tag="VFF28_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45395.1"
                     /translation="KNGYDKSIKDIKVTASIDDLDLEDDSSIANIEPGKTSESKLSFE
                     LPTRIDDGDYKVTIKATGYDSGNNAHTDAWNSELEVKKERHNVQIESAEFSPSLIRCS
                     ETANLDIRLLNLGREDEKVSLKVDNNYLGTVLNEKDIELGSGTDSDADFRRIFPITLP
                     PGIGSATYDFKVTAAYDGRSESSQAKLTIQGCTTEQKLSVKSIPPAAKETLTSTKTLS
                     ATRLSYGPSMALALGLVLIVLLGVIIFLLFVFLMLKK"
     gene            766..1290
                     /locus_tag="VFF28_06980"
     CDS             766..1290
                     /locus_tag="VFF28_06980"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45396.1"
                     /translation="MNIAVCSSGHGDDKEILLKSRKIGSLIAKTGNILLTGGGTGYPL
                     AALMGNIGSKGKAACYSPAKDKEEHESFFKFSIIEGVDYIFTGRGIPGRNYDLVYNSG
                     AIIIIGGRIGSLNEFTAAFALNRKIGILNGSGGIVKVIPEIVDICQLEQKRTVHYSED
                     PKRTDSLSVQVISI"
     gene            complement(1265..1528)
                     /locus_tag="VFF28_06985"
     CDS             complement(1265..1528)
                     /locus_tag="VFF28_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45397.1"
                     /translation="MKKEILRHRPKMQAWKKVVLLLPALLSASWIIFLKYQQSTYVNE
                     MLAPASTGPLIIALAIFTGGYLVFLMLMFSDSIHDTIWKLLGH"
     gene            complement(1525..1881)
                     /locus_tag="VFF28_06990"
     CDS             complement(1525..1881)
                     /locus_tag="VFF28_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45398.1"
                     /translation="MSWGTSATQQGAGCGQASLKLVSYEDGACIKDGTLYVSLYNDGD
                     IALDGVKIGVIGDKMYSNTYYQKIGVAELSKANVEYPVISGVSKVVITPMFFKDSKEN
                     YCGQKGFFIDKIGECQ"
     gene            complement(1953..2216)
                     /locus_tag="VFF28_06995"
     CDS             complement(1953..2216)
                     /locus_tag="VFF28_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45399.1"
                     /translation="MAKEGIIYLQLEKSRIKREKAKIALNIGLVLYFGFLIAGIIGFS
                     FKFIDNIVLNALVISGIIILIVSTLPYLVIAHKEEKWIDEKLR"
BASE COUNT          776 a          535 c          494 g          665 t
ORIGIN      
        1 aaagaatgga tatgataaga gtatcaaaga catcaaagtg acagcctcta tcgatgacct
       61 cgatcttgag gatgattctt cgatagcaaa tattgagcca ggcaaaactt cagaatccaa
      121 gctaagtttt gagcttccca caaggattga tgatggtgac tataaggtca caatcaaagc
      181 aaccggctat gactctggca acaatgcaca taccgatgca tggaactcag agcttgaagt
      241 aaaaaaagaa aggcacaatg tacagataga aagcgcagaa ttttctccct cacttatcag
      301 atgcagcgag acagcaaacc ttgacataag gctccttaac ctgggaagag aggatgaaaa
      361 agtgagcctt aaggtagaca acaactacct tggaacagtg ttaaacgaga aagacatcga
      421 gcttggctca ggaacagaca gcgatgctga ctttaggcgc atattcccca taacccttcc
      481 tccaggcata gggtcagcca cctatgattt taaggttaca gcagcttatg atggcagatc
      541 agagtcttcc caggcaaagc tgacgataca aggctgcact acagaacaaa agctgtcggt
      601 aaaaagcata cctcctgctg caaaagagac attgaccagc acaaagacac tttcagcaac
      661 aaggctttct tacggtccaa gtatggccct tgcacttgga ttggtgctta ttgtccttct
      721 tggagtcata atctttttat tgtttgtttt cttaatgctt aaaaaatgaa tatagcagta
      781 tgcagctcag gccatggtga tgataaagag atactgctaa agtcaagaaa gataggctcc
      841 ttgatagcaa agacaggcaa catcctgttg accggcggag gaacaggcta tcctcttgca
      901 gcactcatgg gaaatatagg atccaaagga aaagctgcct gctattctcc agcaaaggat
      961 aaggaagaac atgagtcttt ctttaagttc tccataatag aaggcgtcga ttacatcttt
     1021 acaggaaggg gaatacccgg aaggaattac gacttggttt acaattcagg cgcaatcatc
     1081 attatcggtg gaaggatagg ctcactaaac gagtttacag ctgcttttgc tctcaataga
     1141 aagataggaa tattaaacgg ttcaggaggt atagtaaaag tgatacctga gatagttgac
     1201 atctgccagc ttgaacaaaa aaggacagtg cactattcag aagatcctaa aagaactgat
     1261 tcgcttagtg tccaagtaat ttccatatag tatcatgtat gctgtcagaa aacatcagca
     1321 tcaaaaatac aagataccct ccagtaaata tcgcaagtgc tatgatcagc ggcccagtgc
     1381 tcgcaggagc aagcatctcg tttacataag tgctttgttg gtacttcaag aatattatcc
     1441 acgatgcgct aagcaaagca ggtagtagca gaacgacctt cttccaagcc tgcatctttg
     1501 gcctatggcg taaaatttct tttttcattg gcactcacct atcttatcga taaaaaaacc
     1561 tttctgcccg caatagttct ccttgctgtc cttaaagaac atgggcgtaa taacaacctt
     1621 actcactcca ctaataaccg gatattcaac atttgccttg ctaagctctg caacacctat
     1681 cttttggtaa taagtattgg aatacatctt atcccctata acacctatct tcactccatc
     1741 aagcgcaata tcgccgtcat tgtaaaggct gacatagagt gtgccatctt ttatacaagc
     1801 gccgtcttca tagctgacaa gcttaaggct tgcttgtccg catccagctc cttgctgtgt
     1861 tgcgctagtc ccccagctca tcaccactcc tccaagggct acagcaaaag ctatgagtat
     1921 gattgtagat attagtggag acatagcctt cttcatctta atttctcatc tatccatttc
     1981 tcttctttgt gtgctatgac aaggtaagga agcgtgctaa cgataagtat tattatcccg
     2041 gatatcacaa gagcattcaa gacgatattg tcgataaatt taaacgagaa acctattatc
     2101 cctgcgataa gaaatccaaa gtagagcacg aggccgatat taagcgctat ctttgccttc
     2161 tcccgcttta tcctgctctt ctcaagctga agatatataa tgccctcttt tgccatagac
     2221 aaaatctcaa tattgattct atataaagct tactgtgtta gggcattgtt aaatcttcgg
     2281 cagattaata tagtacctcg ttctattgac atttgaggtg atattatgaa ggtattttgc
     2341 attttctgca aaaagtcaac aaaccttgtc aaagcaggtt ttcgcatcaa caagacaggc
     2401 aagaaacagc gctacctttg caataactgc aacaggttat ttgtacccga cgatggattt
     2461 tggaagatga
//
LOCUS       DASRUK010000160         5776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1966561, whole genome shotgun sequence.
ACCESSION   DASRUK010000160 DASRUK010000000
VERSION     DASRUK010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1966561"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1387
                     /locus_tag="VFF28_07000"
     CDS             176..1387
                     /locus_tag="VFF28_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004578237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HZX45400.1"
                     /translation="MILTMIVGVVGKANVGKSTFFKAATLAEVEIANYPFATIKPNSG
                     VGYVKIEDPAKPFGKISNPREGFVLNGKRFVPIQLIDVAGLVPGAHDGKGMGNQFLDD
                     LRQADVLIHVVDIAGTTNEKGEPIEPGTYDPKNDILFLDEELDYWILGLLKKGWEKFS
                     RTVQQTNDKIHKALSRHVVGLGVTEEIVLEILNKSDLINKKITEWTESDLMNLGSQIR
                     RKTKPMIIACNKIDVPGAEENFIRIQKEFPGKLMVRCSAESELALREAAKHSLINYIP
                     GEGSFELNDESKLNERQKKALNFIKSDILGKHGSTGVQNILDKAVFELLKYIALFPGG
                     ATKLEDQHGNVIPDCFLMPGGTTALQFAFRLHSDFGNNFIRAIDVKTKRTVGKDHMLK
                     DGDIVEIVANR"
     gene            complement(1388..3085)
                     /locus_tag="VFF28_07005"
     CDS             complement(1388..3085)
                     /locus_tag="VFF28_07005"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HZX45401.1"
                     /translation="MLELLCPAGSLANLKAAIQKGADAVYLGMKKFSARDYATNFNKE
                     YLKQAVKLCKSNDVKLYLAMNTLVKNHELKEYFGQLSYAYSEGIDSVIIQDSSFIDII
                     KKAYPDLNVHISTQAGVMNSAHANLFAKADRITLARELTKEEIARIRKNCKAELEIFC
                     HGALCVSISGSCLFSSFIGGRSGNRGKCAQPCRRRYNNKYLLSTKELCLIDKVPEIAK
                     LNLNAIKIEGRMRTPYYVATVTEIYRKAIDSYYEGNFTITADMRKKLDDAFSREFTQG
                     WFEDSKDIFNMSKAGGRCNINQIREEYKVAVKDIKIDRQKKEVNLSQGRKNSTKKQLL
                     VRVYNKNDALAAQDSGADIIFCDLLNKNFMEIKGSLKCELYGVTPRIMLDNDLPKIKQ
                     AIEEKKPYGLLAGNPGILSLNLDLPLYLDYSLNCFNDISLSSYKDVPIISPELSINEL
                     KRFQNKEFAVLVHGRIRLMTLRHNLPAGVIRDEKAAPFIVNPIYNGTEVLNNKELGLL
                     SKSSQLVSSGINRFFIDTDKEVATIVRLYRQILDNKKVDDGKLKNRYVLGWAYREVV"
     gene            complement(3119..3766)
                     /locus_tag="VFF28_07010"
     CDS             complement(3119..3766)
                     /locus_tag="VFF28_07010"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HZX45402.1"
                     /translation="MNILERLKAVIVSPSKLFNDIKKEDYKPTLIYYLVVVLGLGIFS
                     SLITYATGKAQVELGNLVISWITSIISMFIMAFFIGLVASWFGGDRSWVQGLKAIVYP
                     ASIFAIFGVLMAIAIAVVPSLANLDFQDPQQIMANLGALVGAAVGFGLIALALLVWVI
                     ILYVKGISISSKLSVGKAIGTLIIAGLLAAIVSGMLSWILLKIGLLKPVINAGLK"
     gene            complement(3789..4403)
                     /locus_tag="VFF28_07015"
     CDS             complement(3789..4403)
                     /locus_tag="VFF28_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45403.1"
                     /translation="MADKQKIAVILALLAIIPWVGYLFGLAAIILGIDCLIKEKKKGL
                     AIAALVISIIIISVKISIAITGLLSGFLQPIAEFSQLDYEQALNKCQSQEGDLQSVCY
                     AGLVLLHINDTRVESGDACINITADKTRVECLAFTAIMTNNTDLCLRIEAYDMQETTD
                     AKNYCIAMVTNDELYCSYISDYSQKLSCIQGLKKDQIRNIFGVD"
     gene            complement(4467..4856)
                     /locus_tag="VFF28_07020"
     CDS             complement(4467..4856)
                     /locus_tag="VFF28_07020"
                     /inference="COORDINATES: protein motif:HMM:NF021372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon containing protein"
                     /protein_id="HZX45404.1"
                     /translation="MPHQCVRCSTFYDDGAGEIIKGCKCGGKLFFYVKKEKLDAARRL
                     AEEIKLTPKEKEQIEKDVFDMVGSEIDAEEPVVLDLEAIRVLKPGKYELDLVHLFKGE
                     PLIFKLEEGKYMIDLVESFKKLESKKN"
     gene            complement(4859..5251)
                     /locus_tag="VFF28_07025"
     CDS             complement(4859..5251)
                     /locus_tag="VFF28_07025"
                     /inference="COORDINATES: protein motif:HMM:NF021373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2073 domain-containing protein"
                     /protein_id="HZX45405.1"
                     /translation="MGLILQFVPYSEIEELGPARRVNKLLDIAKQNKIVLLEGRLKKE
                     EEKDLIEITMEEVNNRFKGIELAVINPQKKDIGFIKGIKSGMINMLLGDRQGFTIIGP
                     ATIVKEIKKDPDKIELFTKENGNSKKKK"
     gene            complement(5254..>5776)
                     /locus_tag="VFF28_07030"
     CDS             complement(5254..>5776)
                     /locus_tag="VFF28_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HZX45406.1"
                     /translation="ANRICQDWLGEDMGSVSNIAHETREIHIKEQVTIKSKDGKELSF
                     NLVDNPGIATKIDYEDFLKAGMNEKDAKSRAKEATKGVIDAIKWLDDMDTVVIVLDAT
                     KDPYSQVNITIIGNLQARNIPVLIVANKVDLKKAKIKKIQAAFPQYNVIGISAKYGTN
                     IDQFYEGIFALVG"
BASE COUNT         1547 a         1251 c         1151 g         1827 t
ORIGIN      
        1 catccataat tttgtcaatc caaatatgga acttaaaggc aggacgcctg ccgaggctgc
       61 aggcatcaaa cttgacttgg gaagaaataa gctgctgaaa ttgatagaga tttcatgtat
      121 gccgaagagt taacagtgcc cctttttgaa aaagttttta tagagcggtg aaactatgat
      181 tttgacaatg atagtaggtg ttgtaggcaa ggctaatgtc ggaaaatcca ctttctttaa
      241 agctgcgact ctggcagagg tcgagatagc aaactatcct tttgcaacaa tcaagccaaa
      301 ttcaggggta ggatatgtca agatagagga tcctgccaaa ccgtttggca agataagcaa
      361 tcccagggaa ggatttgtct tgaacggcaa gaggtttgtg ccaatacagt tgatcgatgt
      421 ggcaggcctt gttcctggag cgcatgatgg aaaaggcatg ggaaaccagt tcctcgacga
      481 cctaaggcag gctgacgtgc ttatccatgt cgtcgatatc gcaggcacaa caaacgagaa
      541 gggagagcct atcgagcctg ggacgtatga tccaaaaaac gatatcttat ttttagatga
      601 agagcttgac tactggatac taggactact caaaaaagga tgggagaagt tttcaaggac
      661 tgtacagcag acaaacgata agatccacaa ggcattatcc cggcatgtcg tgggtcttgg
      721 agtcactgaa gagattgtcc tggagatatt aaataaatct gaccttatca acaagaagat
      781 aactgaatgg acagaaagcg accttatgaa cctcgggtct caaataagga gaaagacaaa
      841 acccatgata atcgcatgca ataagataga tgtccccggc gctgaggaga actttatcag
      901 gatacagaaa gaatttccag gcaagcttat ggtaagatgc agcgcagaaa gcgagcttgc
      961 tcttcgtgag gctgcaaagc acagtttgat aaactatatc ccaggtgaag gaagctttga
     1021 gttaaatgat gagtccaagc tgaatgaaag gcagaaaaag gcgcttaact tcatcaagtc
     1081 agacatcctt gggaaacatg gctccacagg ggtgcaaaac atccttgaca aagcagtgtt
     1141 tgagctgctt aagtacatcg ctttgtttcc tgggggagcc acaaagcttg aagaccagca
     1201 tggcaatgtc atccctgact gctttttaat gcccggaggc actactgccc ttcagtttgc
     1261 gttcaggctt cattcggatt ttggcaataa cttcataaga gcaatagacg tcaagacaaa
     1321 gaggactgta ggaaaggatc atatgctcaa agacggagat attgtcgaga tagtggcaaa
     1381 taggtagcta gactacttct ctatacgccc agccaagaac atatctgttt tttagctttc
     1441 catcgtcaac ctttttatta tctagaatct gcctgtaaag cctgacgatg gttgccactt
     1501 ctttgtctgt atcgataaag aacctgttta tcccagagct taccaattgc gagcttttgc
     1561 taagcaagcc aagttcttta ttgtttaaaa cctctgttcc gttatagatc ggattgacta
     1621 taaaaggggc ggctttttca tctcttataa ctccagcggg caagttatgc cttaaggtca
     1681 tcagccttat cctgccatgg accaagactg caaattcctt gttttggaac cgctttaact
     1741 catttatact caactcaggg gatattatgg ggacgtcttt gtatgatgat aggctgatat
     1801 cattaaaaca gttaaggcta tagtcaagat acaatggaag atcgagattt aggctgagga
     1861 ttccggggtt tcctgcaagg aggccgtatg gcttcttttc ttctattgcc tgttttattt
     1921 taggcagatc attgtcaagc attatcctgg gggttactcc atatagctcg catttgagcg
     1981 atcctttaat ttccatgaag tttttgttta ataagtcaca aaagataata tctgctccgc
     2041 tgtcttgcgc tgcgagcgca tcatttttat tgtagaccct gaccaataat tgttttttgg
     2101 tgctgttttt cctgccttgg cttagattta cctctttttt ttgcctgtct atcttgatgt
     2161 cttttactgc gaccttgtac tcttccctta tctggtttat gttgcatctt ccaccagcct
     2221 tactcatgtt aaaaatatct tttgagtcct cgaaccaccc ttgcgtaaat tccctgctaa
     2281 atgcgtcatc gagctttttt ctcatgtcag cagttatcgt aaaattgccc tcataatagc
     2341 tgtctatggc ttttctgtaa atctctgtga ctgttgccac atagtagggt gttcgcatcc
     2401 ttccttcaat cttaatcgcg tttaggttta attttgctat ctctggaacc ttatctatca
     2461 ggcagagctc ttttgtcgat aacaggtatt tgttgttata tctccttcgg caaggctggg
     2521 cacattttcc cctgtttcct gagcgtcctc caataaaact tgagaaaagg caggagcctg
     2581 agatggagac gcataatgcg ccgtggcaga atatttccag ctctgccttg cagttttttc
     2641 taatgcgtgc aatctcttct tttgtaagtt ccctggccaa tgttatcctg tctgcttttg
     2701 caaacaggtt tgcatgagct gaattcatga cgcctgcttg ggtcgatata tggacattta
     2761 agtcagggta tgcctttttg atgatatcta taaaggaaga gtcctgtata atcaccgagt
     2821 caataccctc tgaataggcg tatgaaagct ggccaaaata ttcttttaac tcatggttct
     2881 ttacaagggt attcatcgca agataaagct tgacatcgtt tgacttgcag agctttactg
     2941 cctgcttaag atattccttg ttaaaattag tagcataatc ccttgcgctg aatttcttca
     3001 tgccaagata tactgcatct gcgcccttct ggatagcagc ctttagatta gcaaggcttc
     3061 ctgcagggca taataactct aacatacaaa gaaaaaagaa taagaagtat aaaaaaattt
     3121 actttagtcc agcatttata actggtttta gcaaacctat ctttagcagg atccagctga
     3181 gcatacctga aactatggct gcgagcaatc ctgcaattat caaagttcca atagcctttc
     3241 ctacactaag tttgctactg atggatatgc ccttgacata taatatgatg acccatacca
     3301 ggagtgctaa tgctatcaac ccaaaaccaa cagctgctcc gacaagtgcg ccaagatttg
     3361 ccattatctg ttgagggtcc tggaaatcaa ggtttgccag cgaaggtact actgctattg
     3421 caatcgccat caatacgcca aatattgcga agatgcttgc aggatagact attgccttta
     3481 agccctgcac ccagcttctg tctcctccaa accaggatgc gacaagaccg atgaagaagg
     3541 ccataatgaa catgcttatt atcgatgtta tccaggatat taccaaattc cctagctcca
     3601 cttgcgcttt tcctgtggca taggttatca atgaagaaaa tatacccagc cccaaaacca
     3661 caactaaata atatataagt gtcggcttat agtcttcctt ctttatatca ttaaacagct
     3721 ttgaaggaga aacaataact gctttcaaac gttccaatat attcattttt ttcaatcttt
     3781 tatccatatc aatcaactcc aaatatgttt cttatctgat ctttttttaa tccttgtatg
     3841 cagcttagct tttgactgta gtccgagata taactgcaat aaagttcatc attggtaacc
     3901 atggctatgc aataattttt cgcgtccgtt gtctcctgca tatcatacgc ttctatcctc
     3961 aaacagagat cagtattatt ggtcattatt gcagtgaatg caagacattc gacccttgtt
     4021 ttatcagcgg taatatttat acaggcatct ccgctttcta cccgcgtatc attaatatgg
     4081 agaagcacga gtccagcgta gcagactgat tgaagatctc cttcttgaga ttggcattta
     4141 ttcaaggctt gctcataatc taactgagaa aactcagcga taggttgaag aaaaccagac
     4201 aataaacctg taatcgctat ggaaatctta actgagatga taattatcga tattaccaat
     4261 gcagctatgg caagaccttt ctttttttcc ttgatcaaac agtcaatgcc caggattatc
     4321 gctgcaaggc caaaaaggta gcctacccat gggattatcg ccaatagtgc gagaataacc
     4381 gctatttttt gcttatctgc catagttagg ccaacggtca accgcctata taaacctaac
     4441 tgtttagacg atagaataat cgtggcttag tttttcttgg attcgagctt cttaaaactt
     4501 tctaccaaat ctatcatata ttttccttct tcaagcttga atatcaatgg ctcgcctttg
     4561 aaaagatgga caaggtctag ttcatatttg cctggcttta acacccttat cgcctcgagg
     4621 tcaaggacaa ccggctcttc tgcatcaatc tctgagccga ccatgtcaaa gacatctttt
     4681 tctatctgct ctttttcttt tggagtgagc tttatctctt ctgcaagtct tcttgctgca
     4741 tcaagctttt cttttttaac gtagaaaaat aatttaccgc cgcatttgca gcctttgatt
     4801 atttcgccag ctccgtcatc atagaaagtg ctgcatctga cgcattgatg gggcattttt
     4861 attttttctt ctttgagttt ccgttctctt ttgtgaacaa ctctatctta tcagggtctt
     4921 tctttatctc ttttactatt gtcgcaggac cgattatcgt aaaaccctgc ctgtcgccaa
     4981 ggagcatgtt aatcatgcct gactttatcc ctttgataaa accaatgtct ttcttttgag
     5041 ggtttatcac agcaagctct attcccttaa agcggttgtt tacctcttcc atggttatct
     5101 cgatcaagtc cttttcctct tcctttttta gcctgccttc caataggaca atcttgttct
     5161 gctttgcgat atcaagaagc ttgtttaccc ttcttgcagg cccaagctct tcaatctctg
     5221 agtacggaac gaattggagt atgagtccca tttttatcct accaaggcga agatgccttc
     5281 ataaaactgg tcgatatttg tgccgtattt ggctgagatg cctataacat tatattgagg
     5341 gaaagccgcc tggatctttt ttatcttcgc tttctttagg tcgaccttgt ttgccactat
     5401 aagtacaggt atgtttcttg cctgaaggtt tcctatgatt gtgatgttaa cctggctgta
     5461 tgggtccttt gttgcgtcga ggactattac tactgtgtcc atatcatcca gccattttat
     5521 cgcatcgata actccttttg tagcttcctt tgcccttgat tttgcgtcct tctcattcat
     5581 gcctgctttc aagaaatctt cataatcaat ctttgtagca atacctggat tgtcgacgag
     5641 gttgaatgac agctctttgc cgtcttttga ttttatggtg acttgctctt ttatatggat
     5701 ctctcttgtc tcgtgagcga tgtttgagac agagcccatg tcctctccaa gccagtcctg
     5761 gcagatcctg tttgcc
//
LOCUS       DASRUK010000161         4377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1967283, whole genome shotgun sequence.
ACCESSION   DASRUK010000161 DASRUK010000000
VERSION     DASRUK010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1967283"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="VFF28_07035"
     CDS             complement(<1..934)
                     /locus_tag="VFF28_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZX45407.1"
                     /translation="MYRFLSNIYPRKLKDDYKKTMVYSGIKIDVHKFIGFVIALSFLS
                     SVAAAFFLGYLFEVSFWVTFFLGFFGMQVLAYFLLLIRADSKARFVESILPDVLHLMS
                     SNLRAGLTTDKALLLAARPEFGPFQDEINQVGKEITMGKEISEALAEISKKIKSERLK
                     KTIALIVSGLRSGGELSALLEQTAKNLRQERMVDSRIRANVMMYVIFIFTAVCFGAPM
                     LFGLSSFLVEVMTESLGAIEIPKTAAAQMPISFSDISIDPSFVVFFSCFFLISSSILG
                     ALILGLISKGKEREGIKFMPILIVLSLLVFFLVRF"
     gene            complement(1042..1932)
                     /locus_tag="VFF28_07040"
     CDS             complement(1042..1932)
                     /locus_tag="VFF28_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZX45408.1"
                     /translation="MFKIPYTILPPKVLKDASYSFTGVAEAVEVLFPFLKMHLRQAQV
                     DYSVKEYLRMCLLSSVIFFFFLAFLFIIVLFAAGVSGFLLFGLIVPLVITLFTFFQQI
                     AYPKIHANKRIKDIERNLLAALQNMLVELNSGVPLFGILVNISKADYGEVSNVFSIAI
                     KEINAGKQQIETLEELAAINPSLFFRRAVWQIVNGMKSGADMASVVSEIIDSLSEEQL
                     LQIQRYGSQLNPLAMFYMLTVVIAPSLGMTFLIILSSFISLSEFTLKGIFWGLLGVVI
                     FFQIMFMGLIKSKRPNLLSD"
     gene            complement(1938..3449)
                     /locus_tag="VFF28_07045"
     CDS             complement(1938..3449)
                     /locus_tag="VFF28_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HZX45409.1"
                     /translation="MAESVVEKYEIESEGVKVDVNIKGGKGYTNRYEIIVEQLSRPTV
                     ALIDEIKHELITEVKVSAAEILDPKAIDRLKDKFRSKACELLKEKLPRLEENTKKFLV
                     GILLHEMLGFGEIEFLLNDGNLEEIIINSANEPIRVYHKKYGWLVTNIVVDSEAKIQN
                     FSNMIARRVGRQITVLNPLLDAHLVTGDRANAVFYPISNKGNTITIRKFARDPWTVTD
                     LINNNTSNSSIFALIWMAIQYEMNILISGGTGSGKTSFLNVCTPFMPPNNRIISIEDT
                     RELQLPKYLYWCPLTTRQPNPEGKGEVTMLDLLINSLRMRPDRIILGEMRRRQEAEVL
                     FEAMHTGHSVYATVHADSIHETIQRLTNPPIEIPPNMLGSVNLNVVMFRDRRRGLRRM
                     FQIGEFIVTEEGGEVNIKPNIIYRWKAANDTIIQHSNSVRLFEDLSRHTGLSHSEIQK
                     DLETKENILNYMVKNKIRDLERVGSLFNKYYIDTETLVQSIEKNAKPSALLKD"
     gene            complement(3442..>4377)
                     /locus_tag="VFF28_07050"
     CDS             complement(3442..>4377)
                     /locus_tag="VFF28_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45410.1"
                     /translation="KIRLQLNIIVSSLIIALFIIALASIIINIDDILPIAGKYTYPSI
                     YLMISLFLMSLLPMMGYRPFLKSKAERERIRLERQKSKEKKLRLKEQMRLAKLDIKKH
                     RLIENKKRQQEKERLRKEAQARKEATIKARNIEREKIKKEKEKIKLEKKNQKDAEKER
                     KRKLAEKLREEAPKRRLEKLRLATERRIKHEESKRIRMLEKERRKAEKLKAKEERRKN
                     KEEQKELKKKERVPLVKELSSSLKIDIGKYETDIDILYKIIDKNSRIKLSAIASYFGI
                     DERKAEEWAHILEEHNLAEIHYPPIGGPELRRKNG"
BASE COUNT         1101 a          978 c          838 g         1460 t
ORIGIN      
        1 caaatcttac taaaaaaaag actaacaagc taagcactat tagtattggc atgaacttga
       61 tgccctctct ttctttgcct ttggagataa gtcccagtat caaagctccc agtatcgaag
      121 atgatatcaa aaaaaagcat gagaagaata cgacaaagct ggggtcgatg gatatgtctg
      181 aaaaggatat tggcatctga gcagccgcag tctttgggat ttcgattgcc ccaaggctct
      241 ctgtcatcac ttcaacaaga aatgacgata gcccaaaaag catcggagcg ccaaaacata
      301 ctgctgtaaa tataaatatc acatacatca tgacatttgc ccttatcctg ctgtcgacca
      361 tcctctcttg cctaaggttc tttgcagtct gctctaacag tgcagaaagc tctcctcctg
      421 acctaagtcc tgacactata agtgctatag ttttcttaag ccgttcagac ttgatttttt
      481 tgcttatttc agcaagagcc tcgcttatct ccttgcccat ggttatctct tttcccacct
      541 ggtttatctc atcctgaaat gggccaaact caggccttgc tgcaaggaga agcgctttat
      601 ctgtggtaag ccctgccctt agattgcttg acataagatg cagaacatcc ggaaggatgc
      661 tttcgacaaa ccttgccttt gagtcagctc taatcagcag gagaaagtat gcaaggactt
      721 gcatgccaaa aaagccaagg aaaaatgtta cccagaatga tacctcaaaa agatatccca
      781 ggaagaatgc agcagcgaca gatgagagga aagaaagtgc gatcacaaat ccgatgaact
      841 tatggacatc tatctttatg ccagagtata ccatcgtttt cttatagtca tccttaagct
      901 ttctgggata gatgttggat aagaatctat acatgtcaaa aaccccgtat tgaaatgcta
      961 aagcctgttg tcgagggttt ttgagcatgc tcagaaaccg ctcgctttac cttggttgtg
     1021 tcaatagcgg tttctgacat gttaatcact taggaggttt ggcctctttg acttgataag
     1081 tcccataaac attatctgga agaagatgac gactccaaga agcccccaaa atatgccttt
     1141 aagggtaaac tcagataagg agatgaatga tgataggatg attagaaaag tcatgccaag
     1201 tgaaggcgct atgactacag taagcatata aaacatggcc aggggattta gctggcttcc
     1261 atacctttgg atctgcagca gttgttcttc agataaagaa tctattatct cgctgactac
     1321 gcttgccata tcagcccctg atttcatgcc attgactatc tgccatactg ccctcctgaa
     1381 aaaaagcgag ggattgattg ccgcaagctc ctccaatgtc tcgatctgct gtttgcctgc
     1441 atttatctct tttatggcga ttgagaaaac attggagact tcaccataat ctgccttcga
     1501 tatatttaca aggatgccaa atagtggcac acctgaatta agctctacaa gcatgttctg
     1561 tagcgcagca agaaggtttc tttcgatatc ttttatcctt ttgttcgcat ggatcttggg
     1621 gtaagctatc tgctggaaaa aagtgaaaag ggttataaca agtgggacga tgagtccaaa
     1681 taaaagaaat ccagataccc ctgcagcaaa gaggactatt ataaataaga aagcgagaaa
     1741 aaagaaaaag atgacgctgg ataataggca cattctcagg tattccttga ccgagtaatc
     1801 tacctgcgcc tgcctaaggt gcatctttaa gaaaggaaac aacacttcga ctgcttcagc
     1861 aacgccagta aagctataag aggcgtcctt caagaccttt ggaggcagta ttgtataggg
     1921 tattttaaac atagcgatca atcttttagc agggcagagg gtttggcatt cttttctata
     1981 ctctgtacta aggtctctgt atctatataa tatttattaa acaggcttcc tactctttca
     2041 agatccctta tcttattctt gaccatatag tttaggatgt tttcctttgt ctcaagatcc
     2101 ttttggattt cactatggga tagcccagta tgcctggaca ggtcttcaaa aagcctgact
     2161 gagttcgagt gttggattat tgtgtcattt gcagccttcc acctgtagat tatatttggt
     2221 ttgatgttta cctctccgcc ttcttcggtc acgatgaatt ctccaatctg aaacatcctt
     2281 ctaagacctc ttcttctgtc cctaaacatt accacattca gatttacaga gccaagcatg
     2341 ttaggtggga tctcgatagg agggttcgtc agcctttgga tggtttcatg gatagagtct
     2401 gcatgcactg ttgcatatac gctatgtcct gtatgcattg cctcgaatag aacctcagcc
     2461 tcctgtcttc ttctcatctc tccaaggatt atcctatcag gtctcatcct taggctgttt
     2521 atcaggagat caagcatcgt aacctctccc ttgccttcag gatttggctg ccttgttgta
     2581 agagggcacc aataaaggta ttttggaagc tgcagctctc tggtatcctc tatgcttatt
     2641 atcctgttgt ttggtggcat gaacggggtg cagacattca gaaaagaggt cttgccagac
     2701 cctgttccgc cagagataag tatgttcatc tcatactgta tcgccatcca gataagggca
     2761 aatattgaag aatttgaagt gttgttgttg attaggtctg tcaccgtcca aggatccctg
     2821 gcaaattttc ttatggttat ggtatttccc ttgtttgata taggataaaa cacagcattt
     2881 gccctatccc cagtcacaag gtgtgcatca agcagcggat tgaggacggt tatctgccgg
     2941 cctactctcc tggctatcat atttgagaaa ttctgtatct ttgcttcaga gtctaccact
     3001 atgtttgtta cgagccatcc atatttttta tggtagaccc ttatgggctc gtttgcagag
     3061 ttgattataa tctcttcaag attgccatcg tttaacagga attctatctc tccaaacccc
     3121 agcatctcat ggagcaatat tccaacaagg aatttttttg tattctcttc aagcctgggc
     3181 agcttttctt tgagcagctc gcatgccttt gacctgaatt tatctttcag gcggtcgatt
     3241 gccttgggat caagtatctc agcagcagat acctttacct cggttatcag ctcatgtttt
     3301 atttcatcga taagtgcgac tgtaggcctg ctcaactgtt cgactattat ctcatacctg
     3361 ttggtatacc ccttgccgcc ttttatgttt acatctacct tgacgccttc agattctatc
     3421 tcatattttt cgactactga ttcagccatt cttccttctt agttccggcc cgcctatcgg
     3481 ggggtaatgt atctccgcaa ggttatgctc ctcaagtata tgtgcccact cctcagcctt
     3541 tctttcatcg atgccaaaat agcttgcgat ggcagaaagc tttatcctgc tgttcttatc
     3601 tatgatcttg tataatatgt ctatatctgt ctcatatttg ccgatgtcta tcttgaggct
     3661 tgatgacagc tcttttacaa gagggactct ttctttcttc tttagctcct tttgttcttc
     3721 cttatttttt ctgcgctctt ccttcgcttt tagtttctcg gcttttcttc tttctttttc
     3781 aagcatcctt attctttttg attcttcgtg ctttatcctt ctttcggttg caagcctaag
     3841 tttctccagc cttcttttgg gtgcttcttc cctgagcttc tctgcaagct ttctttttct
     3901 ttccttctct gcgtcttttt ggtttttctt ttcaagctta atcttctctt tttctttttt
     3961 tatcttttcc ctttcgatgt ttcttgcttt tatcgtcgct tcttttctag cctgggcttc
     4021 ctttcttagc cgttctttct cctgctgcct tttcttattc tcaataagtc tatgtttctt
     4081 tatatccagc tttgccagcc tcatctgctc ttttaatctt agtttctttt ccttactctt
     4141 ttgccgttca agcctgatcc tctccctttc agctttgctc ttgaggaaag gacgatatcc
     4201 catcataggt aaaagtgaca ttagaaataa agatatcatc agatatattg atggataagt
     4261 atactttcct gcgataggta gtatatcatc gatattaatg attatagaag caagtgctat
     4321 tataaacagt gctatgatta gggatgaaac tattatattt agttgtaacc ttatttt
//
LOCUS       DASRUK010000162         9345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1322808, whole genome shotgun sequence.
ACCESSION   DASRUK010000162 DASRUK010000000
VERSION     DASRUK010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 9345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9345
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322808"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VFF28_07055"
     CDS             <1..711
                     /locus_tag="VFF28_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012276762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HZX45411.1"
                     /translation="TTLRKLNPSPYMFYINQSDGILLGASPEMSLRVQGEKEKTIEIR
                     PIAGTKPRGIVNEKIDPDLDSRYEAELKIDQKELAEHTMLIDLARNDVAKVSQTGTRF
                     CNEPYVVEKYSHVQHLVSNVSGTLKKDLDALHAYLATMNMGTLTGAPKIEAMKLIRKY
                     EKTRRGFYGGAVGYITPAGDMDTTIVIRSMFLKNNKAYIRAGAGIVYDSIPASEFAET
                     EKKAKACLKAIEIAGGSR"
     gene            708..1283
                     /locus_tag="VFF28_07060"
     CDS             708..1283
                     /locus_tag="VFF28_07060"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HZX45412.1"
                     /translation="MNILFIDNFDSFTYNLVDEFEKRDCKVLVYRNNTDAKTIEQAVK
                     EFKPSLIVLSPGPGTPRQSGICNDILSNYKGKIPIFGVCLGHQCIIEHFGGRVDRAIE
                     TIHGKPSKITHDNIGIFEGVENPFQAGRYHSLVGYDIPYCLEVSARSKRDLVMAVRHK
                     EFKVIGVQFHPESILTPVGGKLIENLLRMIR"
     gene            1280..2287
                     /gene="trpD"
                     /locus_tag="VFF28_07065"
     CDS             1280..2287
                     /gene="trpD"
                     /locus_tag="VFF28_07065"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013798972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HZX45413.1"
                     /translation="MIQQAISKLIDKKDLSIVEAREVMDEIMSGKTTPAQIAGFLVAL
                     RLKGETIDEITAFAQVMRKKAQTIHPKGNLLDVVGTGGDKSGTFNISTAAAFVTAGAN
                     IKVAKHGNKCVSSQSGSADVLTALGININLQPAQVEKCIDEIGIGYMFAPSFHNAMKY
                     AIGVRKELGVRTVFNILGPLTNPAEAKYELMGVFDEKLVEPLAEVLGNLGCKHALVVH
                     GNGLDEVTLDGKTKIAEFEDDHVRTYEIKPTDFKIKLANLQEIKGGSAEQNGKIILDI
                     LHGKEKGPKKDIVVLNSAAAIYAADKASSIKEGIDMAIDSVDSKKALEKLSKLKEWTS
                     R"
     gene            2272..3084
                     /gene="trpC"
                     /locus_tag="VFF28_07070"
     CDS             2272..3084
                     /gene="trpC"
                     /locus_tag="VFF28_07070"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HZX45414.1"
                     /translation="MDQQVSPCFKEDKAMILDSIIEHKKKEVARRKKILPLSSFRNKI
                     TRSKRSFKRAISTSFGLIAEIKRASPSKGLLSNNFDLKKIATIYQNNHHVKAVSVLTD
                     LKYFKTGIHSLSEASKHIEKPILRKDFIIDEYQIYEARRYGADAILLIASILGKIKID
                     RYISIAKKLGMDCLVEVHTKEELKKVLKTKAEIIGINNRNLDTLKIDLNTTIKLAKIV
                     PKGKIIVSESGFKTSADIKKAKSYVNAVLIGTSLIQSKDIDKKIKELMKCVR"
     gene            3072..3716
                     /locus_tag="VFF28_07075"
     CDS             3072..3716
                     /locus_tag="VFF28_07075"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HZX45415.1"
                     /translation="MCQVKICGITNKKDALKAATLGADFLGFLVEIGFSEDKVSRKTA
                     KEIIKSLPLEVSPVFVTYLKKAKPIIEIAQDISPSIIQLHNDIPIEEIGKIRKALPRL
                     KLTKTISVIDESSIKEAKRYEPYVDYILLDSKSGKKKGGTGKTHDWSISRKIVKALNK
                     KVFLAGGLNPDNVREAIGIVNPFAVDTNSGVKENKGKKDSKKLKNFIEAAKNGL"
     gene            3706..4026
                     /locus_tag="VFF28_07080"
     CDS             3706..4026
                     /locus_tag="VFF28_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45416.1"
                     /translation="MDYKVILSVVAGLVLLNNSLYPVLPLPYLAVMAAVILGAGLLLM
                     GMAKGNYIGKANLVIGIFLGIFAITFILGYLAISIPFITYIFAVQKYIYILGGILLLI
                     SPFT"
     gene            4023..5195
                     /gene="trpB"
                     /locus_tag="VFF28_07085"
     CDS             4023..5195
                     /gene="trpB"
                     /locus_tag="VFF28_07085"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HZX45417.1"
                     /translation="MKRFGKYGGIFVPETLMPACQELEKAFNRYKADKRFNAEFTTLL
                     KDFVGRPTPLTYAENMSKRLGCKLYLKREDIAHTGAHKINNALGQALLAKYMGKTRVI
                     AETGAGQHGVATAAAASKLGLKCEVYMGTTDIERQKPNVFRMQLLGAKVVPVDTGSKT
                     LKDAINEALRDWSASVKDTHYLLGSALGPHPYPTMVAHFQSIIGKEARKQILKSEGKL
                     PNTVIACVGGGSNAIGIFKGFINDRKVRLVGVEAGGKGISTKEHAARFADKQLGRPGI
                     IHGTFTYVLQDKFGQIFNTHSVSAGLDYCAIGPEHSYLREIKRAEYGYATDKECLDAF
                     NLLSKEEGIIPALESSHALAYLIKHAKEYKNKLVIINLSGRGDKDLSEVLRLKNER"
     gene            5185..5994
                     /gene="trpA"
                     /locus_tag="VFF28_07090"
     CDS             5185..5994
                     /gene="trpA"
                     /locus_tag="VFF28_07090"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HZX45418.1"
                     /translation="MKDRYKQKFAQLKERKEKALIPFVVAGDPDYNTSLQIVKTIIDS
                     GADILELGFAFSEPIADGPTIHAAGQRALKAGADTDKNFEFVRKIRKYDKHIPIGILI
                     YSNLIYQRSIAKFYKDAKAAGIDSVLVADLPIEEAGEYINHAKKNKVSTVFIVSQLTS
                     DERMKRIAGKTDGFVYVVSRLGVTGARKELGKGTLSLIKRIRPKTSLPICVGFGISAP
                     EQVRSVTKEGADGAIVGSAIVKMIEKNIKNHAELLKSLKDYIKALKKATFR"
     gene            5991..6935
                     /locus_tag="VFF28_07095"
     CDS             5991..6935
                     /locus_tag="VFF28_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45419.1"
                     /translation="MKKIILGLMVIVFASAAYACDLTATLNKDQSKSYTANGNEYNVT
                     FHSLVNNSGDLAVKFKVNGEITNELEKGQKYSFGDLSDITLDEITLATSSTNTSARFC
                     LRSGLSCTDCGLCSSSKQCDDSNACTIDECDGDPLRCKHKLILWCKNDDGCCPKKCSE
                     KNDNDCKINQTTMNSTNSSLINLSLGNISVIPCISNDSICQSNCTYPADTDCSQCDQD
                     QTCDDNKPCTIDVCSGSPKRCSNTAKPGCEKDGKCLSIGVKDNGSYCSQQGSIQSLKK
                     FKDSCSNYYECEDRICRKGVCKKPPILKRVTGWFTSLF"
     gene            6986..8080
                     /gene="trpS"
                     /locus_tag="VFF28_07100"
     CDS             6986..8080
                     /gene="trpS"
                     /locus_tag="VFF28_07100"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HZX45420.1"
                     /translation="MTKTIDPYGSELVADYVKVIKDFGLEPFDIKNYPSPNKLMRRGV
                     VFAERDIKPVLAAMKGKKPFYVLSGIMPTAKKVHLGTKMVVENIKYFQEHGAKTFCLV
                     ADLEAAAARGISLKEARENALEFQIPAYLALGLDPKNTTFYFQSENKEVMHLAYEFSK
                     KITLNEYKAIFGVADPGRIMSALTQAADILYPQLKERMPGVIPVGVDQDPAIRLTRDI
                     VRRTKEKKFFLPSGLYHKFTPSLDGALKMSKSKPESSIDLPDDPKKAVKKVMCAKTGG
                     RKTVEEQRKMGGVPEDCIIFELYKQHLMEDDNDLKKIYDDCRGGRLLCGEDKQICAGL
                     ISRFMENLEKDIDKARKDIDMSEIASGARF"
     gene            8221..8427
                     /locus_tag="VFF28_07105"
     CDS             8221..8427
                     /locus_tag="VFF28_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45421.1"
                     /translation="MWSKVAPLHGSFMIASIVGFLISALYIYKQLGSKTWGFTLMLFF
                     ILMFVASIISMTYAPVMPEMAKKR"
     gene            complement(8790..>9345)
                     /locus_tag="VFF28_07110"
     CDS             complement(8790..>9345)
                     /locus_tag="VFF28_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45422.1"
                     /translation="PITPCAVAKDGKFGLAIKYNGDLIDPFDGYKINPEEFSVYEMGS
                     SGLELMGYNLLAAHLIRKATSYEGMDNAQTKRYATYAHRAVQAGLKVDQNFPLLCAQN
                     GILMCKRGDFKGALPELLKTIKFIDSGDIRTAMGDCYRDSKSYDQARTNYEMAIGMRR
                     RGGGPQMKELVNNIERSIAELPKN"
BASE COUNT         3139 a         1822 c         2064 g         2320 t
ORIGIN      
        1 acaaccctaa gaaagctaaa tccttctcca tacatgtttt acatcaacca gtctgatggt
       61 atcctgcttg gagcctcacc tgagatgtcc ttaagggtcc agggagagaa ggaaaagacg
      121 atagagatta ggccgatagc aggaacaaag ccaaggggta tagtaaacga aaagattgac
      181 ccagacctgg attcaagata tgaagcagaa ctaaagatag atcaaaaaga gcttgcagag
      241 cacacaatgc tcatagacct tgcaagaaac gatgttgcaa aggtttcaca gacaggtaca
      301 aggttctgca acgaacctta tgtagttgaa aaatacagcc atgtccaaca tcttgtatcc
      361 aatgtgagcg gcactttaaa aaaagacctg gatgcattgc acgcatatct agcaaccatg
      421 aacatgggaa cattgaccgg agcaccaaag atagaagcca tgaaactcat cagaaagtat
      481 gaaaagacca ggagaggatt ctacggcgga gcagtcggct acatcacacc agccggtgat
      541 atggacacaa cgattgttat ccgaagcatg ttcctgaaga acaacaaagc ctatataagg
      601 gcaggagcag gtatcgttta cgactctatc cctgcctcag aatttgcaga gacagaaaaa
      661 aaggcaaaag catgtcttaa agccattgag atagcaggtg gttcaagatg aatatcctat
      721 ttatcgacaa ctttgactca tttacctata atctggtcga cgagtttgaa aaaagggact
      781 gcaaggtatt agtttataga aacaacacag acgcaaaaac aatagaacaa gcagtaaaag
      841 aattcaagcc gagtctcatc gttctttctc caggtccagg aacaccgaga caatcaggca
      901 tttgtaatga tatcctttca aattacaaag gaaaaatacc aatttttggt gtttgccttg
      961 gccatcagtg catcatagag cattttggag gcagggtaga ccgggcaata gaaacaatcc
     1021 acggcaaacc ttcaaagata acccacgata acattggaat ctttgaaggt gttgaaaacc
     1081 ctttccaggc aggaagatac cacagtcttg tcggctatga tattccatat tgcctggagg
     1141 tttctgcaag aagcaagaga gatctggtca tggcagtaag gcacaaggaa ttcaaggtta
     1201 tcggcgtgca gttccatcct gaatctatcc tcactccagt cggcggaaag cttattgaaa
     1261 atctcttaag gatgataaga tgattcagca ggcgatttct aagcttatcg acaaaaaaga
     1321 cctttccata gttgaggcaa gagaggtcat ggatgagatt atgtccggta aaaccacacc
     1381 agcccagata gctggatttc ttgtggcact caggcttaaa ggtgaaacca tagatgagat
     1441 aactgccttt gctcaggtta tgaggaaaaa agcgcagacc atacatccaa aaggaaacct
     1501 cttagacgtt gtcggaacag gcggagataa atcaggcact ttcaatatct ccacagcagc
     1561 agcctttgtt acagcaggtg caaacatcaa ggttgcaaag catggcaaca aatgcgtatc
     1621 ctctcaatca ggctcggcag acgtcttgac agcattaggg ataaacataa acttacagcc
     1681 cgcacaggtt gagaaatgca ttgacgagat aggtataggc tatatgtttg ccccttcttt
     1741 tcacaacgct atgaaatatg caattggtgt aagaaaagag cttggagtaa gaacagtatt
     1801 caacatcctt ggccccctca caaaccctgc agaagcaaag tatgagctta tgggcgtctt
     1861 tgatgaaaag ctggtggagc cattggcaga agttctgggt aatcttggat gcaagcatgc
     1921 gcttgttgtt catggaaacg gccttgatga ggtaacattg gacggcaaga caaagatagc
     1981 tgaatttgag gatgatcatg tcaggacata cgagataaag ccaactgatt tcaagataaa
     2041 gctcgcaaat cttcaagaga taaaaggagg ctcagccgag caaaatggaa agattatact
     2101 cgatattttg catggaaaag aaaaaggccc aaaaaaagat attgtagtct taaactcagc
     2161 tgcagcaata tatgctgctg acaaagcatc gagcataaaa gaggggatag atatggcgat
     2221 agattctgtt gattctaaaa aagccttgga aaagctaagc aagctaaaag aatggaccag
     2281 caggtaagtc cttgcttcaa ggaggataaa gcaatgatcc tagacagcat aatagagcat
     2341 aagaaaaagg aagtggcaag aagaaagaag atattgccct taagcagctt tagaaataaa
     2401 ataacaaggt caaagaggtc gttcaagcgc gcaatttcaa ccagttttgg ccttatcgca
     2461 gagataaaga gagcttcacc atccaaagga ttattgtcaa ataactttga cctaaaaaag
     2521 atagcaacca tctaccaaaa taaccatcac gtcaaggcag tctctgtgct tacagacttg
     2581 aagtatttca agacaggcat ccattcgctt agcgaagcat caaagcacat agaaaaacct
     2641 atcctaagga aggatttcat catcgatgaa taccagatat acgaagccag gcgctatgga
     2701 gcagatgcaa tcctccttat agcatccatc ctgggcaaaa tcaagataga ccgttatata
     2761 tcgatagcaa aaaagctggg catggactgc cttgttgaag ttcacacaaa agaagagcta
     2821 aaaaaagtat taaagacaaa ggcagagata atcggcataa acaaccgtaa tcttgacacg
     2881 ctaaagattg atttgaacac aacgataaag ctggcaaaga ttgtcccaaa aggaaaaatt
     2941 atagtcagcg aaagcggatt taaaacgagt gcagatatta aaaaagctaa gtcatatgtg
     3001 aatgcggtct tgataggaac atccctgatc cagtcaaagg atattgataa aaaaattaag
     3061 gagttaatga aatgtgtcag gtaaaaatct gcgggataac caacaaaaaa gatgcgctga
     3121 aggcagcaac ccttggcgca gacttccttg gatttcttgt agagataggc ttctctgaag
     3181 acaaagtctc aagaaagaca gcaaaggaaa taatcaaaag cctgcctttg gaagtgtctc
     3241 ctgtctttgt gacctactta aaaaaggcaa aacccataat tgagattgca caagatatct
     3301 ctccctcgat aatccagctt cataacgata tccctataga agagatagga aagatcagaa
     3361 aagcacttcc aagattaaag ctcacaaaga ccatttcagt catagatgaa agttcgataa
     3421 aagaggcaaa aagatacgag ccctatgtcg attatatcct ccttgactca aaatcaggaa
     3481 aaaagaaggg gggcacaggt aaaactcacg attggagcat cagtagaaaa atagttaaag
     3541 cgcttaacaa aaaagtgttc ttggcaggag gcctaaaccc agacaacgtc agggaagcga
     3601 taggcattgt aaatcctttt gccgtggaca caaactctgg cgtaaaggaa aacaagggaa
     3661 aaaaagatag taaaaagcta aaaaacttta tcgaggctgc aaaaaatgga ctataaggtt
     3721 atcctttcag tggttgctgg actcgtgctc ttgaacaata gtttataccc tgtcctgcct
     3781 ttgccgtatc ttgcagtgat ggctgcagtc atcttaggtg ctggtcttct cttgatggga
     3841 atggctaagg gaaattatat cgggaaggca aatctggtta taggcatttt tctaggtatc
     3901 tttgcaataa ccttcatcct gggctatctt gccataagca tccctttcat aacttacata
     3961 tttgcagtcc aaaaatacat ttatatcctt ggaggtatat tgctgttgat aagcccattc
     4021 acatgaaacg ctttggtaaa tatggcggta tctttgtccc tgaaacattg atgccagcat
     4081 gccaggaact tgaaaaagct ttcaatagat acaaggccga caaaagattt aacgcagaat
     4141 tcaccaccct tttaaaagat tttgtcggaa ggccgactcc tctaacttac gcagaaaata
     4201 tgtctaagag attaggctgc aagctatatc taaaaagaga agacatcgca catacaggcg
     4261 ctcacaagat aaacaatgct cttggccagg ccttgcttgc aaaatatatg ggtaagacaa
     4321 gggttattgc agagacaggc gcaggccagc atggtgtggc gacagcagca gctgcaagca
     4381 aactaggctt gaagtgtgaa gtctatatgg gcacgaccga catagaaagg caaaagccaa
     4441 atgtcttcag gatgcagctc ttgggagcaa aagtagtccc tgtagataca ggctcaaaaa
     4501 cccttaaaga cgctatcaat gaagcgttga gggattggtc tgcaagcgta aaagataccc
     4561 attatctttt aggaagtgca cttggccctc atccatatcc taccatggtc gcccacttcc
     4621 agagcattat cggaaaagag gcaagaaagc agatactaaa aagcgaagga aagcttccaa
     4681 acacagtgat agcatgcgtt ggcggcggct caaatgccat aggcatattc aaaggattta
     4741 tcaacgatag gaaagtaagg ctggtaggag ttgaagctgg tggaaaagga ataagcacaa
     4801 aagaacacgc agcaaggttt gctgacaagc agctcggcag gccaggtatt atccatggca
     4861 ctttcacata tgtgcttcag gacaaattcg gtcagatatt taatacgcat tcagtctcag
     4921 caggccttga ttattgtgcg ataggtccag aacattccta tctgagggag ataaaaagag
     4981 cagaatatgg atatgcgaca gacaaagaat gcctggatgc attcaacttg ttgagcaaag
     5041 aagaagggat aatcccagca ctggaaagct cccatgccct tgcttatctc ataaagcatg
     5101 caaaggaata caaaaacaaa cttgtgatta taaatctctc aggaaggggt gataaagacc
     5161 tttcagaggt gctaaggctt aaaaatgaaa gatagatata agcagaagtt tgcacaatta
     5221 aaggaaagga aagagaaggc tttgattccc tttgttgtag caggcgatcc tgattacaat
     5281 actagtctgc agattgtaaa aaccatcatc gattcaggcg cagatatcct ggagctaggc
     5341 tttgctttca gcgagcctat agctgatggt ccaacgatac atgcagcagg ccaaagagcg
     5401 ctaaaagctg gagcagatac cgacaagaac tttgaatttg taagaaagat aaggaaatac
     5461 gataagcaca tacctattgg aatccttata tactcaaatc ttatctacca aagaagtatt
     5521 gccaaattct ataaggatgc aaaggcagca ggcattgact cagtgttggt tgcagacctg
     5581 cccatagaag aagcaggtga atacataaat catgcaaaga aaaacaaggt aagcactgtt
     5641 tttatagtct ctcaattgac atccgacgaa cggatgaaga gaatagcagg aaaaaccgat
     5701 gggtttgtct atgttgtctc aaggctcgga gttacagggg caagaaaaga attaggaaaa
     5761 gggactttga gcctgataaa aaggattcgt ccaaagacct ctcttcctat ctgtgttggc
     5821 tttggcattt cagctccaga gcaagttagg tctgtaacaa aagagggcgc agatggtgcg
     5881 atagttggtt cggcgatagt taagatgata gaaaagaaca taaaaaacca cgccgaattg
     5941 ttaaaaagct tgaaagatta tataaaagcg cttaagaaag cgaccttcag atgaaaaaaa
     6001 taatacttgg attgatggtc attgtttttg cttcagctgc ctatgcatgt gacctgacag
     6061 cgaccctcaa taaggatcag tcaaagtctt acactgcaaa tggcaacgag tataatgtca
     6121 cattccattc tttggtcaat aattcaggag acctggcagt caagttcaag gtgaacggag
     6181 agattacaaa tgagcttgaa aaaggccaga aatattcatt tggtgatctt tcagacataa
     6241 ccctcgatga gataacactt gcaacttcat cgaccaatac ttcagcaagg ttttgcctaa
     6301 gaagcggcct ttcctgtacg gattgcggtt tgtgttcatc cagcaagcaa tgtgatgaca
     6361 gtaatgcctg cactatcgat gaatgtgatg gcgacccctt aagatgcaag cataagctta
     6421 tcctgtggtg caagaatgat gatggttgtt gtccaaaaaa atgcagcgaa aaaaatgata
     6481 atgattgcaa gataaatcaa actacgatga attcgacaaa tagctctctt atcaatctat
     6541 ccttaggaaa catttcagta atcccctgca tatcaaacga ctcgatctgt cagtcaaatt
     6601 gcacctaccc agcagacact gattgcagcc agtgtgacca agaccaaacc tgcgatgaca
     6661 ataagccctg cactatcgat gtatgcagcg gctcacccaa aagatgctca aatactgcaa
     6721 agcctggctg tgagaaagat ggcaaatgcc tttcgatagg agtcaaggat aatggcagct
     6781 attgtagtca gcaaggcagc atacaatcct taaaaaaatt taaggattca tgcagcaact
     6841 actatgagtg tgaagatagg atctgtagga aaggcgtctg taaaaaaccc cctattctta
     6901 aaagagtaac aggctggttt acttcattgt tctaggacat cacatgcaac aagatttata
     6961 aattgacaat ctatttccct atgctatgac aaagactatc gatccctatg gctcagagct
     7021 ggttgctgat tatgtgaagg tgattaagga tttcggcctg gaaccttttg atataaagaa
     7081 ttatccttct ccaaacaagc tgatgaggag aggagtggtt tttgcagagc gggatattaa
     7141 acctgttttg gcagccatga agggaaaaaa gcctttttat gttctatccg gcataatgcc
     7201 tacggcaaaa aaagtgcatc ttggcacaaa gatggtagtg gaaaacataa aatactttca
     7261 ggagcatgga gcaaaaacat tctgccttgt tgcagacctt gaggcagcag cagcaagggg
     7321 gataagcctt aaggaagcaa gggaaaatgc actggaattt cagatccctg catatttggc
     7381 attaggcctt gatccaaaaa atactacttt ctatttccag tcagaaaata aggaagttat
     7441 gcatctagcg tatgaatttt caaaaaagat caccttaaat gagtataagg ctatctttgg
     7501 agtggcagat ccgggcagga tcatgtctgc acttacccag gcagcagata tattgtatcc
     7561 tcagcttaag gaaaggatgc ctggcgtgat tcctgtcggt gttgatcagg atccagcgat
     7621 aaggttaaca agggatatag tcagaaggac aaaagaaaag aagttcttcc ttccttcagg
     7681 attataccat aagtttacac cctctcttga cggcgcatta aagatgtcaa aatcaaaacc
     7741 agaatcatcc attgatcttc cagacgaccc aaaaaaagct gtcaagaaag tgatgtgtgc
     7801 aaaaacaggc gggagaaaga cagttgaaga gcaaagaaaa atggggggtg ttccggaaga
     7861 ttgtataatt ttcgaattat ataagcagca ccttatggaa gatgataatg atctgaaaaa
     7921 gatatatgac gattgcaggg gaggaaggct tctttgcggt gaagacaagc agatatgtgc
     7981 aggattgatc tcaaggttta tggagaatct ggaaaaggat atagacaagg caagaaaaga
     8041 tatagatatg tcagaaatcg ctagtggcgc acgcttctga tgctttgcct cagagaaagc
     8101 caacgatttc tgagcatgct caagaacacg tcccgacctt taggtcggcg gttcttgaca
     8161 agataaacct agtaaagttt aaatagcaca ccctcagaag taagccttga ggtgatagtt
     8221 atgtggtcaa aggtcgctcc attacatggc tcgttcatga ttgcttctat tgttggtttt
     8281 ttgatatctg cattgtatat ctacaagcag ttaggaagca agacctgggg atttacattg
     8341 atgctgtttt tcatattgat gtttgtggcg tcaataatat caatgactta tgctccggtc
     8401 atgcctgaga tggcaaaaaa gagataaatt atggcaaatg acttggatat ttgtaacttg
     8461 gttataacaa acgcatcgaa tatttgtaag taaaccggtg cgtttgctta ttacgaaaag
     8521 catgctttga gtataccttc aaaaagtcct ttgcttagat agaaatgcta tgcatttctt
     8581 gcggtatata aagaatagaa ttctcttttc cactggaaat agatccgcta actatctaag
     8641 ttagcgagcc tgttgtgcag aagtccatgt ttgttggaaa gattccagaa ccctgtttac
     8701 ttgccattta ttgaataccc ttccccaagg atgcaatgag gcaggggaac tctgtgacta
     8761 attatcagtc acttggcgga gaaaaatgat caattctttg gcaattccgc aatacttctt
     8821 tcaatgttat tgaccaattc cttcatttgc ggtccacccc ctcttctgcg catgccaata
     8881 gccatttcat agtttgttct agcttgatca tagcttttgc tatctctata gcagtctccc
     8941 attgctgttc gaatatctcc agaatcgata aattttattg tctttagaag ttcaggtaaa
     9001 gctcccttaa agtcacctct cttacacatc aagataccgt tctgcgcgca caaaagaggg
     9061 aaattttgat caactttcaa tccggcttgc actgccctat gggcataagt tgcatacctt
     9121 tttgtttgcg cattatccat gccttcatag gaagtcgctt ttctgataag gtgcgctgca
     9181 agcagattat atcccattaa ctcaagacca gaactaccca tttcatatac tgaaaactcc
     9241 tccggattaa tcttatagcc gtcgaatgga tcaataaggt cgccgttata ttttatcgct
     9301 aggccaaact ttccatcctt ggctacagcg caaggggtta tagga
//
LOCUS       DASRUK010000163         2284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_654851, whole genome shotgun sequence.
ACCESSION   DASRUK010000163 DASRUK010000000
VERSION     DASRUK010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654851"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..324
                     /locus_tag="VFF28_07115"
     tRNA            join(201..203,228..286,311..324)
                     /locus_tag="VFF28_07115"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:262..264,aa:Pro,seq:tgg)
     gene            355..618
                     /gene="rpl37ae"
                     /locus_tag="VFF28_07120"
     CDS             355..618
                     /gene="rpl37ae"
                     /locus_tag="VFF28_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013645749.1"
                     /note="structural models have indicated that the folded
                     zinc-finger motif interacts mainly with domain III of 23S
                     rRNA, whereas the amino-terminal region of L37 interacts
                     primarily with domain II; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37ae"
                     /protein_id="HZX45423.1"
                     /translation="MAEVKKYTSTKRFGARYGRKMKERFGKIDAIQRQKQKCPYCSAV
                     QVKRVAAGIWSCRRCKTKFTGKAYSISTKILTKEPVAEEVKNG"
     gene            611..748
                     /locus_tag="VFF28_07125"
     CDS             611..748
                     /locus_tag="VFF28_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015733664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit P"
                     /protein_id="HZX45424.1"
                     /translation="MVEYKCFSCNKKVAQDYIRKKVRCPYCGSKILFKPRTVITKVKA
                     R"
     gene            750..1082
                     /locus_tag="VFF28_07130"
     CDS             750..1082
                     /locus_tag="VFF28_07130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit beta"
                     /protein_id="HZX45425.1"
                     /translation="MAELSKETEHKIAQLQLYEQSLQNILVQKQQFQSQQVELDSALR
                     ELGATDKAYKIIGNIMVASDKEELKKDLESKKEAVAIRIRTFEKQEDQIRERAKRIQE
                     EVSKKIKG"
     gene            1084..1332
                     /locus_tag="VFF28_07135"
     CDS             1084..1332
                     /locus_tag="VFF28_07135"
                     /inference="COORDINATES: protein motif:HMM:NF015632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0147 family protein"
                     /protein_id="HZX45426.1"
                     /translation="MDETNPAIDQLAELEEDTNIPKNIKVKIRAIIDILKPEEEVSIK
                     VNKALSELDDMSNDPNLDSYTRTRMWNVVSILEKINHH"
     gene            complement(1319..1573)
                     /locus_tag="VFF28_07140"
     CDS             complement(1319..1573)
                     /locus_tag="VFF28_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45427.1"
                     /translation="MNNKGLSPLLATIMLVVFALIIGTITMNWGKGYIGKLGEAEGKT
                     IVISPQEIDTPLKQLQIEYITGEITEEEYFAREKELFNGG"
     gene            complement(1570..1866)
                     /locus_tag="VFF28_07145"
     CDS             complement(1570..1866)
                     /locus_tag="VFF28_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45428.1"
                     /translation="MSTLAYCFGNVAPFYNLALVAVVIYLFIRLFQKADKKMFIKPWK
                     LVFSAVLIYVFEEIITVLDMSGTLDIPAVTFPLLETAIISIFIYGLLLQKEKVK"
     gene            complement(1866..2153)
                     /locus_tag="VFF28_07150"
     CDS             complement(1866..2153)
                     /locus_tag="VFF28_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45429.1"
                     /translation="MQYIEGILKIANLILAVLAGVIGASLINVCRKKKELRPWLLLIG
                     ALIFFAVQEILGALRAFKVFTSPYLTHIVPTIILVLLVVAVVEQLHLTRGK"
BASE COUNT          754 a          457 c          505 g          568 t
ORIGIN      
        1 aacgcaaatt atatggcgca tctgagggct gaatatatta atggagattt tgagcaccat
       61 tatggcctta ggcataatgc tttgattgag aacgctgcga cttaaccaat tttaagtgtc
      121 cgcacacaca cgattaccaa attccgataa gtttaaaaag caaagcctac cttctgtata
      181 gcaaaactgc ccaatagaac gggattatag taaggctata acgttaagct atggggtagt
      241 ttggcataat cctttccgct ttgggagcgg acgaccccgg ttcaaatgcc gcagaaaaag
      301 cggctgaatg tccgggtagc cccattcata gtaataagta aggagagtag taaaatggct
      361 gaagtaaaaa aatatacatc gacaaaaagg tttggtgcaa gatacggcag gaagatgaag
      421 gaaaggtttg gaaagataga tgccatccaa aggcaaaagc agaaatgccc gtattgctca
      481 gctgtccagg taaaaagggt agcagcaggc atctggtcat gcaggagatg caagacaaag
      541 ttcacaggaa aagcatattc tataagcacg aagatattga cgaaagagcc agtggcagaa
      601 gaggtcaaaa atggttgaat acaaatgctt tagctgcaac aagaaagttg cacaggacta
      661 cataagaaag aaagtcaggt gcccgtattg cggcagcaag attcttttta agccaaggac
      721 tgtcattacc aaagttaagg caaggtaaga tggcagagct ttcaaaagag accgagcata
      781 agatagcaca gcttcagctt tatgagcaaa gccttcagaa catccttgtt caaaagcagc
      841 agttccagtc ccagcaggtc gaattagact ctgcattaag ggagctgggg gcgacagata
      901 aggcatacaa gataatagga aatattatgg tggcttcaga taaagaagag ctaaaaaaag
      961 acctggagtc aaaaaaagag gctgtggcaa taaggataag gacctttgaa aagcaggaag
     1021 accagataag ggaaagggca aaaaggatcc aggaagaagt ttctaagaag attaaaggtt
     1081 aatatggatg agacaaaccc cgctatcgat caattggcag agcttgagga agatacaaat
     1141 attccaaaga atatcaaggt aaagataaga gctatcatcg acattctaaa gccagaggaa
     1201 gaagtgtcta taaaggttaa caaggcgcta agtgaacttg atgatatgtc aaatgaccca
     1261 aatcttgact cttatacgag aacccggatg tggaatgtgg tttcaattct agaaaagatc
     1321 aaccaccatt aaacagttct ttttccctgg caaaatattc ctcttcagtt atctctcctg
     1381 tgatatattc tatctggagc tgctttaatg gagtgtctat ctcctgcggg cttatcacta
     1441 tcgtctttcc ttctgcttca ccaagtttgc ctatatatcc tttcccccag ttcattgtga
     1501 tagtgccgat tataagcgca aagactacaa gcatgatagt tgcaagcaga ggtgaaagtc
     1561 ctttattgtt cattttacct tttccttttg gagtagtagt ccgtatatga atatgcttat
     1621 tatcgctgtc tcaagtagcg gaaaggttac tgcagggata tcaagcgtcc ctgacatgtc
     1681 aaggacagtg attatctcct caaagacata gattagcact gcagagaaca caagcttcca
     1741 tggtttgatg aacatcttct tgtcagcctt ttgaaagagc ctgatgaaaa gatagatgac
     1801 gacagcgaca agagcaaggt tatagaaagg cgctacattc ccaaagcaat aagctagcgt
     1861 cgacattact ttcccctcgt caggtgtagt tgttcgacca ctgcaacgac aagcagcaca
     1921 agtattattg tcggaactat atgtgtaagg tacgggcttg taaaaacttt aaacgcgcga
     1981 agcgctccaa gtatctcttg cactgcaaag aagatcaatg ccccgataag caacagccat
     2041 ggcctaagct cttttttctt cctgcacaca ttgataaggc ttgcgccaat aactcctgca
     2101 agcaccgcta gaatcaagtt agctatctta agtatccctt cgatatattg catctcacac
     2161 ctctttatcc cttactctgc gatagctcat ttaaatattt tgccaaaaat aaaggccata
     2221 ttctgaaaca ttagctctgc caacaatgat ggcttcgacg ccttgctgcg caaggctgca
     2281 aacc
//
LOCUS       DASRUK010000164         7823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_798656, whole genome shotgun sequence.
ACCESSION   DASRUK010000164 DASRUK010000000
VERSION     DASRUK010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 7823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7823
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798656"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            614..1879
                     /gene="dnaG"
                     /locus_tag="VFF28_07155"
     CDS             614..1879
                     /gene="dnaG"
                     /locus_tag="VFF28_07155"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase DnaG"
                     /protein_id="HZX45430.1"
                     /translation="MGKISPVSSKYIVNASIEIDGIVDRPDVIGAIFGQTEGLLGADL
                     ELRELQRSGRIGRIEVNVDTKTGKTAGSITIPSSLDKAETSIVAAALEIIQRIGPCNA
                     KIRVQNIEDIRVTKRSFVIERAKELLKTMMDGVIPDSQELADEVAYSVRVMEIQEYGK
                     DRLPAGPGIDESDEVIVVEGRADVLNLLKHGFKNAVAMNGTSIPETIVELARKKVVTA
                     FVDGDRGGRLIIKDLINSTEIDYVAMASAGKEVEEITKKEIHKALRGRVSVEQVKLEL
                     GKDVDRIEKPPMYEPRNEQRRPQPSYSKPQRPFIQKPAAPMRQAKREATPEEKKVFKE
                     MLENLVGTRGAHILDDKLNILGKVPITELQTTIKSLSSGVYAIIFDGSIERELVKTAE
                     RAHITFLVGMDMKLRPEETPIELLTVNEL"
     gene            complement(2441..3601)
                     /locus_tag="VFF28_07160"
     CDS             complement(2441..3601)
                     /locus_tag="VFF28_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45431.1"
                     /translation="MADEDIKSRLEQELNFLEESYQAEVISKEEYNKGKARIEKKLSE
                     LNAGEEYLEIKVSEDITEEAPVQVESADDIIKEAARPVEEKKEGLEQNAEELCEKSLD
                     ATQEVDQKEEPNEEKAEGPKEKSSIVKYVYVAAVLSLMVLLIFSLKSCGKDEAIPAAA
                     GQCSADSDCIKESLAGTCVSPGTESAECRFKEDVKANLMVINDQDCALCSTDSALSII
                     KEQFPNLNIDELDYKSSKTLVNDLRINSLPAYVFDGNITKTANFAGFEPALINVKDFY
                     VLSEKASGSAYFFRRPQVKNKLDIYLSPSTASLIEPRIAEIINLFGTKLNVSVYSSTA
                     AKAKEELAINTYPTFVLNNQYKFKGILPANAIKEKICAVSILDECKEELSKV"
     gene            complement(3603..4685)
                     /locus_tag="VFF28_07165"
     CDS             complement(3603..4685)
                     /locus_tag="VFF28_07165"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HZX45432.1"
                     /translation="MPQDKEDKAKEEPKKKKKSAKLKKSTVKKISAVIVLLVVLGVIL
                     FFALRTLRPEQVVAQVNDEKITKRELDAKFAQLPEQYKIFLTKDNFLDQVINVKLLLQ
                     ESEKQGIRVTEAELEAELDSLRKMAPTEEDFQKLLQQQNLGLEDLKDQLREQIEINKL
                     LNITVLSKITVAEQKIRDYYQANKEYFDKSNLTYNGSKPQIKQILLNDISSNAAEIYI
                     NQLRVDAFIKIGRKQITTKVETFTKVEDPICKDNGKPILRLYSTRSNSASKWISKSYE
                     KVASEYEGKVVVYYWQLDTGDDLLTAGKETGLPPSEVEIFKKYSPKNAVPVFVFGCKY
                     IRVANGYDTLEGEEAEFRRIIDRLLV"
     gene            4865..7480
                     /locus_tag="VFF28_07170"
     CDS             4865..7480
                     /locus_tag="VFF28_07170"
                     /inference="COORDINATES: protein motif:HMM:NF026240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HZX45433.1"
                     /translation="MYEIVIGRSEKDRLKYGTEGAIFLGKHYVKMGQATSLSNNIYMD
                     ITRSHVVFVCGKRGSGKCLTGDTLITLENGLEVPVKDLCETNDRVFGLDQDLKIKPFA
                     RTEFFQREVDEIIKVKLKSGKEIKLTSEHPLLSIDGWKECRELKIGSRIATPRIIRCN
                     YKEKMEDHEIKVLAYLIAEGHVKKHLTFSNYDKVLVEDLRDSLLRMEPFMELVELRKG
                     CYRINSRNIKRKVNGYPINRDKTGRFGKGSSINHEKMRQSLEADNIHGLLPAQKFIPK
                     RIFTTHKEGIKLFLNRLFSCDGSIYKKSNKPYWEISYASSSERLIKEVQSLLLRFNIL
                     SKSRVKEIKCNLFQTFELVLNGESVITFIREIGFFGTKKDKEAIALKDMEAIRKNTNV
                     DTIPKEIWNNYRPKSWSDVGKTFGYKTPKALRSSIAYSPSRQKLLQIAIADQNERLRC
                     LAQSDIFWDEIVYMEKIEGKFPVYDITVPEAHNFIANNIIVHNSYTMGVIAEGMSDIP
                     PEIRDNIAIIMLDTMGIYWTMKYPNDKEEELLKEWNLAGKALDTVIYTPTGYYKKYKE
                     EGVPTDKPFSIKPSELDASDWCSTFEIGITTEEGVLIESIINDLRDTVKDFDIGEVID
                     AVNNYGRAKQETKNAVINRFLDSKRWGIFSREGTPLNELVKGGQVTVLDVGCYATIPG
                     TQGLRALVIGLIAEKLFVQRMIARKNEELRSIKETTHFMGDNAEGKNKEPLVWLVIDE
                     AHEFLPKEGETVATHPLVIVLREGRQPGISLILASQQPGKIHTDVMTQSDIVIAHRIT
                     AKIDIEALGALMQSYMREGLDKELNNLPRNKGAAIVFDDTNERMYSMRVRPRFTWHGG
                     DSPTAMHKAKKVFDF"
     gene            7531..7779
                     /locus_tag="VFF28_07175"
     CDS             7531..7779
                     /locus_tag="VFF28_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45434.1"
                     /translation="MVDQDESLSKIPLSRRVVDAGVAALLFGYVATTAFKKGRRWLFS
                     RHSLSNILTTPSLKSCFVFEIVAYPAHCFSNGLEIAVL"
BASE COUNT         2412 a         1513 c         1664 g         2234 t
ORIGIN      
        1 gctcatgggc tgcataagaa atgaaaatgg cagaaggttt attggaaatg tgatgtcggc
       61 aatggaaaca tggaatcagc aagggaaaaa tgtctatgaa aatctgattt catttgctag
      121 ctgactgaat acttactcac aacatataca tttatataat agttcatagt taccaacact
      181 taaagattaa agaagcccgt cacgagagta cccaatcgag ctgacgctta atgcaagcaa
      241 tgaacttggg ggttagtgct gcgggctcca atcatattta taagcttgat taaggctaaa
      301 accgaacaag tttaggatca tccaatcgtc tggaaaccaa acggtttctg acatgtcaaa
      361 aactctgcat tgagaagcgg agcgagctgt ggaggagttt ttgagcgtgc ccagaaatcg
      421 ctggcttgct ctaggctaaa attgccaaag agtgcgtcaa aagcgatttc tgacatatca
      481 acaattcatc cataaggtaa attctagact tgttctaaga aggagaagat caaaatcaca
      541 atcatcactt caatgtacga agaagatttg tgttagatga actccttctg aagcttatag
      601 gagagtgctt aaaatgggaa agataagtcc ggtttcatcc aaatatatcg taaatgcatc
      661 aatcgagata gatggaatag tcgataggcc agatgtcatc ggcgccatct ttggacagac
      721 agaaggtctt cttggtgcag accttgaatt acgtgagttg caaagaagcg gaaggatagg
      781 tagaatcgag gtcaatgttg acacaaaaac cggcaagact gctggaagta tcacgatccc
      841 ttcaagcctg gataaagcag agacatcgat cgttgcagct gcgcttgaaa taatccaacg
      901 aataggacca tgcaatgcaa agatcagggt ccagaacatc gaagatataa gggtcacaaa
      961 aagaagcttt gtcattgaaa gggcaaagga actattaaaa acaatgatgg atggagtcat
     1021 ccctgattcc caggagcttg ctgatgaagt ggcttattct gtaagggtca tggagataca
     1081 agagtatggt aaggacagac tgcctgccgg cccaggaatt gatgaaagcg acgaagttat
     1141 tgtcgttgag ggcagagctg atgtgcttaa cctgctaaag catggattta agaacgctgt
     1201 cgccatgaac gggacttcga ttcctgaaac aatcgtggaa ctcgccagga aaaaggtagt
     1261 gactgctttt gtcgatggag ataggggagg aagattgata atcaaggatc ttatcaattc
     1321 cactgagatt gactatgtgg cgatggcttc agccggcaaa gaggttgaag agataacaaa
     1381 gaaagagatc cacaaggcgc tcagaggaag agtttctgtc gaacaggtca agcttgaact
     1441 tggcaaagat gttgacagga tagagaagcc tccgatgtat gagcccagga acgaacaacg
     1501 aaggccacag ccaagctatt caaaacctca gaggccattt attcaaaagc ctgctgctcc
     1561 gatgagacaa gcaaagaggg aagctacccc cgaagagaag aaagtcttta aggaaatgct
     1621 tgaaaatctg gtaggcacaa gaggggcaca tatacttgat gataagttga acattttagg
     1681 taaggtgcca atcactgaac ttcagacaac aatcaagagc ctgtcatctg gcgtctatgc
     1741 aataatcttt gatggaagca tagaaagaga gctagtaaag acagctgaaa gggcgcacat
     1801 aacattcctt gtcggaatgg acatgaagct aaggccagaa gagacgccaa tagaactttt
     1861 gactgtgaat gagttataaa ttagagaaca tctgttctca attttttctt tattttattt
     1921 gattaattat acattagtat atatgccaag aatcaaggga ttagctaata cccatttaga
     1981 taataattaa attgtaacta taattaaagc ttgaattatt gcaataggaa acattccata
     2041 tatctctttc ttttgtaaaa atgaagcaat ataaccacat tcaaggcaat aatccataca
     2101 aatatagagg aatgttggtt ttatatttat actgcaaaaa ccaaattggt aaagatatca
     2161 gccaaagaca taagttcagt ccaatcattc cacataatcc gattaatatt tccctcaatt
     2221 tattatcgga agcagaacca ttcatacatt ctttatttag acgggattat ttaatgtttt
     2281 tggcattatt aactctactg cgcttagtca aataaaaatc ccaaccgccc caagggtttt
     2341 agtgttgtaa ataccctcaa cgtggcgagc cataacaata acccagtcac cgcaagtgag
     2401 tggggtattt acaacaaata aaaagaaact attaaagaat tcaaaccttt gacaattctt
     2461 ccttgcactc gtccaagatc gaaacagcac atatcttttc ctttatcgca tttgcaggaa
     2521 gtattccttt gaacttatac tggttattca agacaaatgt cggataagtg ttgatggcaa
     2581 gctcttcttt tgccttggca gctgtgctgg aatagacgga gacatttagc tttgttccaa
     2641 aaagatttat tatctcggct atccttggct caatgagtga agctgttgac ggcgatagat
     2701 agatgtcgag cttgtttttt acctgaggcc tcctaaagaa gtatgcgctg cctgaggctt
     2761 tttccgacag aacataaaaa tccttaacgt ttatcagcgc aggctcaaag ccagcaaagt
     2821 tagctgtttt tgttatattg ccgtcaaaga cataggctgg caatgaattt atcctgaggt
     2881 cgtttaccag cgtttttgag gatttataat ccaattcatc gatattcagg tttgggaact
     2941 gttctttaat tatagatagc gcactatctg tactgcacaa agcgcaatct tgatcgttga
     3001 ttaccatgag gtttgctttt acatcttcct taaatcgaca ttctgcgctc tctgtacctg
     3061 ggcttacaca tgtgcctgcc agggactctt ttatgcagtc actatctgct gagcactggc
     3121 ctgctgccgc gggaatggcc tcatctttac cgcagctttt taacgagaaa atcaataaga
     3181 ccatcaatga caagaccgct gcgacataaa catatttaac gatggatgac ttttcctttg
     3241 gcccttctgc cttctcttca tttggctctt ccttttgatc tacctcttgt gtcgcatcca
     3301 ggcttttttc acataactct tctgcatttt gctctagccc ttcctttttt tcttcaacgg
     3361 gccttgccgc ctctttgata atgtcgtctg cggattcaac ctggacaggg gcttcttcag
     3421 ttatatcttc actaactttt atctctaggt attcttcccc tgcatttaac tcggatagct
     3481 ttttttctat gcgtgccttg cctttgttgt actcttcctt tgagataacc tctgcctggt
     3541 aactctcttc aaggaaattt aattcctgct ctagcctgct ttttatgtct tcgtctgcca
     3601 tttcaaacca acaacctgtc tattatcctc ctgaattctg cctcctctcc ttccaatgta
     3661 tcatatccat ttgcaaccct gatatattta caaccaaaaa caaagacagg aacagcgttc
     3721 ttcgggctgt acttcttgaa aatctctacc tcgcttggag gcaggcctgt ctcttttcct
     3781 gcggttaaca gatcatcccc tgtgtcgagc tgccaataat ataccacaac cttgccttca
     3841 tattcagagg ccactttttc ataagacttg cttatccact ttgacgctga atttgacctt
     3901 gtgctataaa gccttaatat tggttttcca ttgtccttgc agatagggtc ctccaccttt
     3961 gtaaatgtct ctactttggt agtaatttgc ttcctgccta ttttgataaa agcatctact
     4021 ctcagctggt tgatatagat ctctgctgca ttgcttgaaa tatcatttaa caatatctgc
     4081 tttatctgag gctttgaccc attgtaggtg aggttgctct tatcaaaata ctctttgttt
     4141 gcctgataat aatcccttat cttctgctct gctactgtaa tctttgacag gaccgtaatg
     4201 ttaagaagtt tgtttatctc tatctgttcc ctcaactggt cttttagatc ttcaagccca
     4261 aggttttgct gctgcaaaag cttctggaag tcttcctctg ttggagccat cttccttaag
     4321 gaatcaagtt ctgcctcaag ctctgcttcg gtgaccctta ttccctgctt ctctgattcc
     4381 tggagcaaaa gcttgacatt tatgacctga tccagaaaat tgtcctttgt aagaaagatc
     4441 ttgtattgtt caggcagctg ggcaaatttt gcatccagct ctcttttcgt tatcttttca
     4501 tcatttacct gcgccactac ctgctcaggc ctcaaagtcc tcaatgcaaa aaaaaggatg
     4561 acaccaagaa ccacaagcag aactatgact gcagatatct tctttacagt gctttttttt
     4621 aacttagctg atttcttctt ctttttgggc tcttcctttg ccttatcttc cttgtcttgt
     4681 ggcatttttt aatcccctct ctaagagttt aataagcaac ctagaacccc ctttttatga
     4741 tttgaatagt acacctttta tttaaaactt gtgatttgac caatccatag atgaatcact
     4801 gattacttta ccacctgata gtttcgataa acctttatct aattactgct tggcaataag
     4861 gagtatgtat gaaattgtga taggaaggag tgaaaaagac aggctaaagt atggaacaga
     4921 aggggcaata tttcttggaa agcattatgt caagatgggg caggcgacct cgctttcaaa
     4981 taacatctat atggatatca caaggagcca tgttgtcttt gtctgcggaa aacgtggaag
     5041 tggaaaatgc cttactggag ataccctcat cactcttgaa aatggccttg aagtgccagt
     5101 aaaggactta tgcgagacca atgatagggt ttttgggctt gatcaagact taaaaataaa
     5161 gccatttgca aggacagagt tttttcaacg agaggttgat gagataataa aggtaaagct
     5221 gaaaagcggc aaggaaataa agctaacttc cgagcatccc ctgctttcaa ttgatggctg
     5281 gaaggaatgc agggaattaa agatcggctc aaggatagcc actcctcgca taatcaggtg
     5341 caactataag gaaaagatgg aagaccatga gataaaagta cttgcttacc tgatcgccga
     5401 agggcatgta aaaaagcact tgaccttcag caattatgat aaggttttgg tagaggatct
     5461 aagagattcc ctattgagga tggaaccatt catggagctg gttgagttaa gaaagggatg
     5521 ttataggata aattccagaa atatcaagag aaaggtgaat ggctatccaa taaatagaga
     5581 taaaacaggt agatttggaa agggctcatc aatcaatcat gagaaaatga ggcaatctct
     5641 tgaagcagat aatatccatg ggctgttgcc agcacagaaa ttcatcccaa aaaggatatt
     5701 tacaacccat aaagaaggga taaaattatt tttgaacagg cttttcagct gcgatggaag
     5761 catatataaa aaaagcaaca aaccttattg ggaaataagc tatgcgtcct cttctgaaag
     5821 acttatcaaa gaagtacaaa gcctgctgtt aaggttcaac atattatcga aatctagggt
     5881 caaggaaata aaatgcaacc tttttcagac ttttgagctg gtattaaacg gtgagagtgt
     5941 aatcactttt atcagagaga taggattttt tggaacaaaa aaggataaag aagcaatcgc
     6001 cttgaaggat atggaggcga taaggaagaa taccaatgtt gataccatcc ctaaagagat
     6061 atggaacaac tataggccaa aatcctggag tgatgttgga aaaacatttg gctataaaac
     6121 tcctaaggcg ctgagaagca gcatagctta ttctccttca agacagaaat tgctgcagat
     6181 agccattgca gatcaaaatg agcgattaag atgccttgcc caatcagata tcttttggga
     6241 tgagatagtt tatatggaaa agatcgaggg aaagtttcca gtatatgata tcactgtgcc
     6301 agaagcccat aactttatcg ccaataatat tatcgtccat aacagctaca ctatgggtgt
     6361 catcgctgaa ggaatgagcg acatacctcc tgagataagg gataatatcg cgataataat
     6421 gctggataca atggggatct attggaccat gaaatatccc aatgataaag aggaagaact
     6481 ccttaaagag tggaatcttg caggcaaggc actggatact gtgatctata cgcccaccgg
     6541 atattataaa aaatacaaag aagaaggagt gcctacagac aagccatttt cgataaaacc
     6601 gtcagagctt gatgcttcgg actggtgctc gacctttgag atagggataa caactgaaga
     6661 gggagtcctt atcgagagca tcatcaacga tcttagggat acagtaaaag attttgatat
     6721 aggcgaggtt atcgatgcgg taaataatta tggcagggca aagcaagaaa caaagaatgc
     6781 agtgataaat cggttccttg acagcaagag atggggaata ttcagcaggg aagggactcc
     6841 cttaaatgaa cttgtgaaag gcgggcaggt aactgtgctt gatgtgggat gctatgccac
     6901 aatccctggc acacaagggc tgagggctct tgtcataggg cttatcgcag agaaattgtt
     6961 cgtgcaaagg atgattgcga gaaagaatga ggaactcagg tcgataaaag agacaacaca
     7021 cttcatggga gataatgcag aaggcaagaa caaagagccg cttgtatggc ttgtgataga
     7081 tgaagcacat gagtttttac ctaaagaggg tgagactgtg gcgacgcatc ctctggtaat
     7141 agtcctcaga gaaggcaggc agccaggtat ttcccttatc cttgcttcgc agcagcccgg
     7201 taagatacat acagacgtta tgacgcagag cgatatagtc atagcgcata ggataactgc
     7261 caagattgat atcgaagcac ttggcgctct catgcaaagc tatatgagag aagggcttga
     7321 caaagagctc aataatttgc caaggaataa gggagctgcc atcgtctttg atgataccaa
     7381 cgaaaggatg tattcgatga gggtgaggcc aaggtttaca tggcatggag gcgactcccc
     7441 aactgcaatg cataaggcaa aaaaagtgtt tgatttctag ttgttacttt atagggataa
     7501 atttatatat ggttaaatat ttctataatt atggtcgatc aagatgaatc attgagtaaa
     7561 attccattaa gtagaagagt ggttgatgca ggtgttgcag cacttctatt tggttatgtc
     7621 gcaacaactg cattcaaaaa aggaaggaga tggctttttt ctcgccatag cttatcaaat
     7681 atattaacta ccccaagtct taaatcttgt ttcgttttcg aaatagttgc ttatcctgct
     7741 cattgcttct ctaacggttt ggaaattgct gttttgtaag atgtctttgt ctaattttga
     7801 gaaaactctt tcaatcatat tca
//
LOCUS       DASRUK010000165        10880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_659651, whole genome shotgun sequence.
ACCESSION   DASRUK010000165 DASRUK010000000
VERSION     DASRUK010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 10880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10880
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659651"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2602)
                     /locus_tag="VFF28_07180"
     CDS             complement(<1..2602)
                     /locus_tag="VFF28_07180"
                     /inference="COORDINATES: protein motif:HMM:NF017411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45435.1"
                     /translation="MRTSIKITILLIYLAAALPFIYGYETWNYVNNAYITGYSPSCNP
                     YQGLRFLYGANLNSGTAVLNCGGFSKSFNGQGYIMWISDMSNPCNATLTVVTDSKTII
                     SSMPYHVREGIYSIEDGKLNGLPVCPQGQSCFDGVCCTPDCSCSVNTCSGSACSDSCG
                     GFCEGTKQPECSCSVDTCIGQICSDGCGEVCEGKKACKSGEPKVRKVTYEYDLYGNIV
                     KAANLGDIYLTGDEKFSYTEYLYNTDDWIINKPRRNYLKDDASLVSETKFAYDGKNYG
                     SYPTKGDLTYEEYWLDSGPSPVNSYDYDSYGNVITHTNPRGYAITYTYDPTNSFLTRT
                     ANAKGHQINYAYDPGTGNLLSSKDANGYITEYAYDTFGRKIKEVLPYDTQNYPTLEIK
                     YYLDGAAPEQIEIIQREVGGTSEAHESYSYLDGFLIAVQNKEETEGSQLTTRDITYDP
                     LYRIKEQSNPYKSSLGYTNPQNVSRKVYSYDSLGRITQTTNPDGTAKKVDYNLWDSTT
                     YDEKGDQKDYTQDAHGRIISVKEYDGQQVYETRYGYDALNNLKEIIDSKGNKIAYGYD
                     SLGRKTGLHDKDIGSWSYSYDSNGNLVRQTDSKGHSTYLSYDELDRLSKKSSPGIDTE
                     YFYDIVTIGTLYKITHPGIETIFQYDSRLRLIDEQKKIEGIVFNAHYSYDSMDRLASK
                     TLPDNSVIEYSYDKEGFVDSIEGLIPHIEYNEMGQPTKRTYDNGIVTNLQYDSDNHRL
                     RRIVTGDKQDLSYSYDDVGNVIRISDSIKGKERLFAYDGLDRLISAKRSSDFDIDYGY
                     DSIGNMLGLSSERLNISFGYSSLPVHSPSSIVYKDSCDVDGDAAKGASCHGDDCDDDN
                     PDIFPGALEIC"
     gene            complement(2608..5979)
                     /locus_tag="VFF28_07185"
     CDS             complement(2608..5979)
                     /locus_tag="VFF28_07185"
                     /inference="COORDINATES: protein motif:HMM:NF023677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin TcdB middle/N-terminal domain-containing
                     protein"
                     /protein_id="HZX45436.1"
                     /translation="MKKVVINRILSLALLAILLYQPVVLAKETEPGNFAFSGQLMPLD
                     MIGLSRSASSGPASPTPTVISDHNMIKDLSGDALNRYTPSGSYSTDLFSGSAAYSYPI
                     QVPPGTNGLQPSISLGYNHHQTALRGVLGNAWSLTQNYIYRDINGTLENTADDAFKLH
                     FNGLDMKLVFAGGYYHTEIENYWKIERVAGGNNFLKEHWILRTKDGKAYRFGYNYDSE
                     FMSNQGIISTDWPEFASIWSLDSIKDTHGNEISYDYDENPSADYYPYLYKISYNRGSN
                     IITFNYDYDSFSGFDGYAYGARIRQNAILNSIVVSSGGNLVRRYGFEYQSISHKKFLN
                     IVRFFGNDNSSELPPMRFSYHMASKGWISDPNYVIPSEAYFGEHKDNGVRVLDINGDG
                     YDDVIKMQNTNNMDYWLNNKYNGWLPKQSLSSLLAGGIVNDWGQDLGVRFFDFDQDSR
                     IDIIKLLKDVSTVKKILINSGNGWKEKGMEISEDIAFIKKNEASERCEPELCPYDYTD
                     EGISCSNGICTRSCSIMACNGMGTAVLDESTNDPGWSDDSYEDWDDGRGFTPQSNKCY
                     KFEFTGSKQTDGDDSDCYDLYTDDRYVDGDYDADCNGRDIDAYAGVGFAGKGTSSTWL
                     STIFDAGDNYGYAGDATNPAWNYFYISKYDNDDKPDDEGSNKGDWDGFDYTLCDEGGT
                     HTIYCTPSYGACRSWGVQRCGYGCAAETTYPYVVIGLYAGYNNNLWDALLDNYGCTEN
                     MIGSNSYYGKNNNYKVTEYDTSKFYKYYQCNYAVDEYVDTGIRLADLNTDGRLDIIRS
                     TEAEKSIWLNTGAEFIPINSTKVPSEAVFADIGIKKDGGTRIVDLNADGLPDLVKGKD
                     GKRITWINNGREWMKDDTWLIPADAVFIENGSDKGVLIIDVDGDGLVDILRADPSVRK
                     AWINTGRGWEEDPSWSIPSSLNFIGPSLTIADVNADGLPDLLMASSSENKASWTNAAS
                     KGHLLDEIINMFGGITRISYEKMASLDNTGEDDVSDLPFGGWVVASITKDNGLSGSHK
                     IISTTNYHYSGGLFDAGEKEFRGFNTVDEILPDGAAIRHVFNQDKGKKGLESKRLFFG
                     FDSNLYQKIEYNWFYEHTQGYYEVYLKDITDYTYR"
     gene            complement(5984..6292)
                     /locus_tag="VFF28_07190"
     CDS             complement(5984..6292)
                     /locus_tag="VFF28_07190"
                     /inference="COORDINATES: protein motif:HMM:NF023376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HZX45437.1"
                     /translation="MKKMNQEMTKMTRNEKIREDEFLANSYRIMFNIENFVITFKQAV
                     CVEKIQNKKKYRLSHLPIILDINDAKRLHSTLKKAVERYEEKYGIIDQLKKSDSSEVD
                     "
     gene            complement(6264..6548)
                     /locus_tag="VFF28_07195"
     CDS             complement(6264..6548)
                     /locus_tag="VFF28_07195"
                     /inference="COORDINATES: protein motif:HMM:NF023376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HZX45438.1"
                     /translation="MEREIVEQKYLREFYAKGFDIALSQKEFCLDFQLVIPLGKVFPE
                     EGEPIHIERHQPIVVIPVIGKLMMKALENSIGEFERKYGAIDEKDESGDD"
     gene            complement(6570..7916)
                     /locus_tag="VFF28_07200"
     CDS             complement(6570..7916)
                     /locus_tag="VFF28_07200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HZX45439.1"
                     /translation="MSKRLIAFLFLSLLINLPICFSAELSLTYDSNGNLVNDSKLYYE
                     YNSLNQLVRVKELNGRVLEEYSYDAAGQRIKKVTYNHDGSNIKVYYVDENYVRIINNS
                     GIFDTKYFYHNGQLVARQDPEGDVYYYHPDHLGSTNLVTDALKSVVEETTYLPFGGIF
                     EGGSSRFTFTGKEKDYSGLMYYGARYYSPFLRRFTQPDTVIQNVYDPQSLNRYSYARN
                     NPLKYVDPSGNYFSPWDILDYISLTISVADVLKNPANIRNWINLGIDVSSAVIPGLGG
                     GGMIYRAATKADDVVDVSKFAKLTKNAKNVGKAKKGVEAADISGDLGEVIQRTQKVPK
                     GHYGEFGEFPDNGLDGLLRNSDNTIDNALPTRNGVQKAQETYKEHLYSHPEYKQRWDG
                     EVPGSDARAFNPWQHIDQDYQASNIPDISSLPGGEQIVIRSPEDEIWEAIYQVRYG"
     gene            complement(7992..9407)
                     /locus_tag="VFF28_07205"
     CDS             complement(7992..9407)
                     /locus_tag="VFF28_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45440.1"
                     /translation="MKSTMVIFICILLVPLASALKTGIVIESESKIYSGCVDVGSGSS
                     VYDVLKKLDGIRDDIKMTIDGELTGMYYLRSVNGLDPKTDGNKYYGWSFFITSEKNDQ
                     FTDPPNFYGIGDYKITKEEAIGIKYGYTEFASDWSIVVPPEKPAFKNSKDLCNNLKVN
                     ITDIYIDGIKQGESITKRGKIKDVFPGSDLEIRIHVENNYDSAAKLDITDINIAGIIE
                     DLIDGDDTEEEIADFDLPAERRTTKSLKFKIPFEVGAKDRFLRIETKAKDDAGIQYSI
                     ESAMDLEIKKESHRLKILEAKLDKNAYSCGINGFLLMDLANIGANKEKVDLQISNEEI
                     GLNREESFELSQDINEASNRYKEQFNIWIPENISKNVYPITITADYGIIKEKEVVNLV
                     VDTCNTKEEVASEPDIVGAELVSKTAGAELNEKKQYNAVNSNVNKRDANKSLTLLLSI
                     VLIILVILMIAGLVVVINHIR"
     gene            complement(9404..9739)
                     /locus_tag="VFF28_07210"
     CDS             complement(9404..9739)
                     /locus_tag="VFF28_07210"
                     /inference="COORDINATES: protein motif:HMM:NF025833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HZX45441.1"
                     /translation="MAVSIALSGCKEQPASGEATLVIDSLGSVKTERFKFSYGTPLGL
                     LKYNHSVDTLFEGSFIECIDGICADGKYLWLLYVNNKTINYGAKKYRLADGDIVKFQF
                     SNKHIGGKR"
     gene            complement(9708..10391)
                     /locus_tag="VFF28_07215"
     CDS             complement(9708..10391)
                     /locus_tag="VFF28_07215"
                     /inference="COORDINATES: protein motif:HMM:NF042461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6580 family putative transport protein"
                     /protein_id="HZX45442.1"
                     /translation="MSQKLVLKSKQEKKLTLKQKIALQAVQKQLQLLRMKEWLVVVGF
                     VFGGAALRAAMQPIPSAEPITFFAILAGWLFGKKKGFITGAAAGYLSNFLMFGGQGPW
                     TIFQMLSWGFAGFLGGFIKGIKPQKSYWWFWLKSLLPLTAVVIASTLFFEIVMNVSWA
                     IMFPFSILGLFISALPFIAVHLVSNIAFTSVLPFARKFAFEKGKFNEMEICKSIIGRF
                     GSKHRSERL"
     regulatory      complement(10389..10512)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01689"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="The adenosylcobalamin-variant (AdoCbl-variant)
                     riboswitch is found in the 5'-untranslated regions of
                     vitamin B12 (Cobalamin) related genes in bacteria; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF01689"
BASE COUNT         2919 a         2513 c         1954 g         3494 t
ORIGIN      
        1 tgcagatctc gagagcgccc ggaaaaatat ctggattatc atcatcgcaa tcatccccgt
       61 ggcaagaagc tcctttggca gcatctccgt caacgtcgca ggaatccttg tatactatgg
      121 agcttggtga atgtaccggc aggctgctat agccaaatga gatattaagc ctttcagaac
      181 tcaaacctaa catattgcct atactatcat agccataatc aatatcaaaa tcagagctgc
      241 gcttggcact gataagcctg tctagaccgt catatgcaaa caagcgttcc ttgcctttaa
      301 ttgaatcgct tatcctgata acattgccca catcatcgta actataggac agatcctgtt
      361 tatcgcccgt gactattctt ctcaaccgat ggttatccga gtcgtactgc agatttgtta
      421 ctatgccatt atcataagtt cgtttagtcg gctgccccat ctcattatac tcaatatggg
      481 gaataagccc ctctattgaa tcaacaaatc cttctttatc atatgaatac tcgatgactg
      541 agttatccgg aagcgttttt gaagccagcc tgtccatcga gtcataacta tagtgggcat
      601 taaaaactat accttcgatt ttcttttgct catcaattag gcgcagcctt gaatcatatt
      661 ggaaaatagt ctcaatgccc ggatgagtta tcttatataa agttccgatg gttactatat
      721 catagaaata ctcagtatct ataccgggtg agctcttttt cgaaagcctg tctaattcat
      781 cgtaagataa gtatgtggag tgccctttgc tgtcagtctg ccttacaaga ttgccattac
      841 tatcataact gtagctccag cttccgatgt ctttgtcatg aaggccagtt tttctgccaa
      901 gagaatcata gccatatgct atcttgttac ccttgctatc tattatctct tttaagttgt
      961 tcagggcatc gtatccatat cgagtttcat agacttgctg cccatcatat tcctttactg
     1021 agattatcct gccatgtgca tcctgggtat agtccttctg atcgcccttt tcatcatagg
     1081 tagtgctatc ccataggtta taatcgactt ttttggccgt gccgtcaggg ttggtggttt
     1141 gggtaatcct gcccaaggaa tcatatgagt atactttcct gctcacattc tggggatttg
     1201 tataacccaa gcttgattta tagggattgg attgttcctt aatcctatac aatgggtcat
     1261 atgtgatatc ccttgttgtt aactgagagc cttcggtttc ttccttattt tgcacggcga
     1321 ttaaaaaacc atcaagataa gagtaacttt catgagcttc tgaggttcca ccaacctccc
     1381 tctgtattat ttctatctgt tccggagctg ctccatctaa ataatactta atctctaaag
     1441 tgggataatt ttgagtatca taaggaagca cttcctttat ttttcttcca aatgtgtcat
     1501 aagcatattc agttatataa ccatttgcgt ccttagaaga taatagattt ccagtccctg
     1561 gatcataggc atagtttatc tgatggcctt tggcatttgc agtccttgtt aaaaagctat
     1621 ttgttggatc gtaggtatat gtgatagcat atcctcgcgg atttgtatgg gttattacat
     1681 tgccataact atcataatca taactattta ctggacttgg gcctgaatca agccaatact
     1741 cctcgtatgt caagtctccc ttggttggat aagagccgta atttttgcca tcgtatgcaa
     1801 acttagtttc actcacaaga gatgcatcgt ctttgaggta attccttctt ggtttgttga
     1861 tgatccaatc atctgtgtta tataggtatt cagtatacga aaatttctca tcccctgtca
     1921 aatatatatc gcccaaattt gctgccttta ctatattgcc gtataaatcg tattcatagg
     1981 tcactttcct tacctttggc tcaccacttt tacaggcctt ttttccctcg cagacctctc
     2041 cacagccatc agagcatatt tgccctatgc atgtatcaac agagcaggaa cattctggtt
     2101 gttttgtccc ttcgcaaaag ccaccgcagc tgtcactgca agcacttcct gagcaagtat
     2161 taacagagca tgagcagtca ggtgtgcagc acactccatc aaaacaagat tggccttggg
     2221 gacaaacagg aaggccattt agcttaccat cttcaataga atatattccc tctcttacat
     2281 ggtaaggcat cgaagaaatt atcgttttac tatcagtcac aacagtaagt gttgcattac
     2341 agggattgct catatcactt atccacataa tgtaaccctg gccattgaat gatttgctaa
     2401 aaccgccaca gttcaagaca gctgtacctg agtttagatt agccccatat agaaatctta
     2461 gcccttgata agggttgcag gagggggaat atcctgtgat atatgcattg ttaacataat
     2521 tccaggtctc atagccatag ataaagggta aagccgctgc taagtaaatc agcaaaattg
     2581 tgatttttat gcttgtcctc atgggcctta cctgtaagta taatcagtga tgtctttaag
     2641 atagacttca tagtagcctt gagtatgctc ataaaaccag ttgtactcaa tcttctgata
     2701 aaggtttgaa tcaaagccaa aaaaaagccg tttagactcc aatcctttct ttcctttgtc
     2761 ctgattaaag acatgcctaa tagcggcccc atcgggcaga atctcgtcca cagtattaaa
     2821 acctctaaac tccttctccc ctgcatcgaa gagccctcct gaataatggt aattagtcgt
     2881 tgagatgatc ttatggctgc cgctcaggcc attatcctta gttatagaag ccacaaccca
     2941 gccgccaaat ggcagatcag agacatcatc ttctcctgta ttgtcaagag atgccatctt
     3001 ttcataactt atccttgtaa tcccaccaaa catatttatt atttcatcaa gtagatggcc
     3061 ttttgatgca gcgtttgtcc aagaggcctt attttctgat gagcttgcca tcaaaagatc
     3121 aggaagcccg tcagcattta cgtctgcgat cgtcaaggag ggcccgatga aattcagtga
     3181 acttggtatg ctccaggagg ggtcttcttc ccatcctctt ccggtattta tccatgcctt
     3241 tcttacagaa ggatctgccc ttaggatatc gaccaaacca tctccatcga catctatgat
     3301 aagaacgcct ttgtcgctgc cattttctat aaacacagca tcggctggga taagccaggt
     3361 gtcgtccttc atccattccc ttccattatt gatccacgtt attcttttgc catcctttcc
     3421 ttttacaagg tctggcaggc catctgcatt aaggtctact atccttgtac ccccatcttt
     3481 ttttattcct atgtctgcaa aaaccgcctc tgagggtacc ttggtgctat ttataggtat
     3541 aaactcagcc ccagtattaa gccagatact cttttcagcc tctgtggatc tgataatatc
     3601 cagccttcca tcggtattaa ggtcagcaag ccttattcct gtgtcaacat attcatcgac
     3661 tgcatagttg cactggtaat acttgtagaa tttggatgtg tcgtattctg taactttata
     3721 gttattattt ttcccataat agctgttgct gcctatcatg ttttcagtgc aaccgtaatt
     3781 gtcaagaagg gcatcccaaa gattattatt ataacctgca taaagaccta tcacaacata
     3841 aggataagta gtttcagcag cgcagccata accgcatctt tggacacccc atgatcggca
     3901 ggctccatag ctgggagtgc agtatattgt atgcgtgcct ccttcatcac agagagtata
     3961 gtcaaatcca tcccagtcgc ctttgtttga gccctcgtca tccggcttat catcattgtc
     4021 atattttgat atatagaaat agttccaggc aggatttgta gcatcacctg cataaccata
     4081 attatctcct gcatcaaaga tagtactcag ccaagtgctt gaagtcccct tgccagcaaa
     4141 tcctacaccc gcgtaagcgt ctatgtctct tccattgcaa tcagcatcat agtcaccatc
     4201 gacgtatcga tcatctgtat agaggtcata acagtcagaa tcatcaccat ccgtctgctt
     4261 gctgcctgta aactcaaatt tatagcattt attgctttgg ggagtaaacc ccctgccatc
     4321 atcccaatcc tcgtagctat catccgacca tcctggatca ttggtgcttt catccaatac
     4381 ggcagttccc atgccattgc atgccattat cgagcatgat ctggtgcaaa tcccgttgct
     4441 acagcttatc ccttcatctg tataatcata agggcaaagc tctggttcac acctttctga
     4501 cgcttcattt tttttaatga atgctatatc ctcagagatc tccataccct tctctttcca
     4561 gccatttcct gaattaatga gtatcttctt cacagtcgac acatccttta atagcttgat
     4621 gatatcaatc cttgaatcct ggtcaaagtc aaaaaatctc actcccagat cctggcccca
     4681 gtcattgact attcctcctg ctagtagact tgacaggctt tgtttgggca gccatccgtt
     4741 atacttatta ttcagccagt aatccatatt gtttgtattt tgcatcttta tcacatcatc
     4801 atagccgtcc ccattgatgt ctaaaaccct tacgccgtta tccttatgct cgccaaagta
     4861 agcttcagaa ggaataacat aattggggtc acttatccat ccctttgagg ccatatggta
     4921 gctgaacctc ataggaggaa gctctgaaga gttgtcattt ccgaaaaacc ttacgatatt
     4981 gaggaatttt ttatggctta tcgattggta ttcaaatcca tatcttctta ccaaattgcc
     5041 accgctgcta actacaatac tatttaagat ggcattttgc cttatccttg cgccgtaagc
     5101 atagccgtca aatcctgaaa aggaatcata gtcatagttg aatgtgataa tgtttgatcc
     5161 tcgattatat gatatcttat ataaataggg ataatagtct gctgacggat tttcatcata
     5221 atcataagat atttcgtttc catgtgtatc tttgatagaa tcaagactcc atatgctcgc
     5281 aaactcgggc caatcagtcg agattattcc ttgattagac atgaactctg aatcgtaatt
     5341 atagccgaac ctatatgcct taccgtcttt tgttcttaat atccagtgtt ccttaaggaa
     5401 gttatttcct cctgccaccc tttctatctt ccaataattc tctatctctg tatgataata
     5461 tcctccggca aacaccagct tcatgtccag gccgttgaaa tgaagcttaa acgcatcatc
     5521 agctgtgttc tccaaggttc cgtttatatc ccgatagatg taattctgtg tcaggctcca
     5581 tgcattacct agtactcccc ttaacgcagt ctggtgatgg ttgtagccga gcgaaatact
     5641 gggctgaagg ccatttgttc cagggggaac ctggataggg taagaatatg ctgcagagcc
     5701 tgaaaacagg tcggttgaat agctgccgct tggagtatag cggttgagtg catcgcccga
     5761 gaggtccttt atcatattgt ggtcgctaat tacggttggc gtaggtgatg ctggtcctga
     5821 tgaagccgac cttgataatc ctatcatatc caaaggcatc aactggcctg agaaggcaaa
     5881 gttgcctggc tctgtctctt tggcaagtac tacaggttga taaagtagga tggccagcag
     5941 agcaagcgac agtatcctat ttatcaccac ctttttcata gggtcaatca acctctgatg
     6001 aatcggattt ctttagctgg tctattattc catatttctc ttcatatctc tcaacggctt
     6061 tctttagcgt agaatgcagc ctttttgcat catttatatc taagataatt ggcagatgac
     6121 tcaacctgta tttcttctta ttttgtattt tttcaacaca aacagcttgc ttgaatgtta
     6181 tcacaaaatt ttcaatatta aacatgatcc tataagaatt agccaagaat tcatcttccc
     6241 taattttttc atttcttgtc attttagtca tctcctgatt catctttttc atcaatggca
     6301 ccatactttc tttcaaactc tccgattgaa ttttctaatg ctttcatcat caatttgcca
     6361 atcacaggta taacaacaat gggctgatgc ctttcaatgt gaattggctc cccttcttct
     6421 ggaaatacct tgccaagagg aatgaccaat tgaaaatcta aacagaattc tttttgtgac
     6481 aaagctatat caaatccctt tgcataaaac tcccttaaat atttctgctc gacaatttcc
     6541 ctttccattt ttacctcctc cttgtagttc tatccatatc ttacttgata aatcgcctcc
     6601 caaatttcat cttctggaga ccgaataact atttgctctc ctcctggaag agagcttata
     6661 tcaggtatat tggacgcttg ataatcttga tctatatgct gccaaggatt aaaggctctc
     6721 gcatcagatc cggggacctc cccgtcccat ctttgtttat attctggatg actgtaaaga
     6781 tgctctttgt atgtttcctg tgctttttgc actccatttc gagtaggaag agcattatca
     6841 atagtattat cagaatttct caataagcca tctaacccgt tgtctggaaa ttcgccaaac
     6901 tctccataat ggccctttgg gactttttgc gttctttgga taacttcccc caaatccccg
     6961 cttatatccg cagcttctac tcctttctta gccttgccga cattttttgc gtttttagtc
     7021 agctttgcaa acttggaaac gtcgacgaca tcatctgcct tggttgcggc ccgataaatc
     7081 atgcctccgc cgcccaaacc aggaataact gcagagctca catcaatgcc cagattgatc
     7141 cagttgcgta tgtttgccgg attttttaga acatcggcaa cagagatggt caggctgata
     7201 tagtctaata tatcccaagg gctgaagtaa ttcccactgg gatcaacata ctttaaagga
     7261 ttgttccttg cgtaactata cctattcagg ctttgcggat catagacgtt ctgtataact
     7321 gtatcgggct gtgtaaaacg ccgtagaaaa ggagagtaat accttgctcc ataatacata
     7381 agcccgctgt aatctttctc ttttccggta aatgtaaacc tgctgctgcc tccctcaaag
     7441 atgccgccaa agggcaggta ggtcgtctct tccactactg actttagcgc atctgtgacg
     7501 agattagtgc tgccaaggtg atcgggatga taataataaa catccccttc aggatcttgc
     7561 cttgcgacca gttggccatt gtgatagaaa tatttagtat caaatatccc cgaattattt
     7621 ataatccgaa catagttctc atcgacataa taaaccttaa tattggatcc gtcatgatta
     7681 tatgtgactt tctttatcct ttggccggcc gcatcatagg agtattcttc aaggacacgt
     7741 ccattgagct ctttaacacg tactagttgg tttaggctgt tgtattcata atacaattta
     7801 gaatcattga ccaggtttcc gttagaatcg tatgttaagg ataattcagc agaaaaacat
     7861 ataggtagat ttatgagaag acttaagaat aaaaaagcaa tcagtctttt gctcatttaa
     7921 cccccattac tagttaaaat tgcttaggca tataaatctt attatcacct ctttgttata
     7981 cccccaataa cctatctgat atggtttata acaactacta acccagcaat catcaaaatg
     8041 acaaggataa tcaatacgat gcttaacagc agagtaaggc tcttgtttgc atcgcgcttg
     8101 tttacattag agtttactgc attgtactgt tttttttcgt tgagttctgc gccagcagtt
     8161 ttggagacca attctgcccc aacaatatcc ggctctgaag ctacctcttc ctttgtatta
     8221 catgtatcaa cgacaagatt tacaacctcc ttctctttga taatgccata atcagctgta
     8281 attgttatcg gatagacatt cttgctaata ttttccggaa tccatatgtt gaactgttcc
     8341 ttatatctgt ttgatgcttc atttatatcc tggctaagtt caaagctctc ttcacgattt
     8401 aatccaatct cctcgtttga tatctgcaga tcgaccttct ccttatttgc ccctatattt
     8461 gcaagatcca taaggagaaa cccattaatc ccgcatgaat atgcattctt gtcaagcttt
     8521 gcctcaagga tctttagcct atggctctct ttctttatct ccaagtccat tgcagattcg
     8581 attgaatatt gtattcctgc atcatccttt gcctttgttt ctatccttaa aaaacggtct
     8641 tttgccccta cttcaaatgg aattttgaat ttcaatgatt ttgtggtccg cctttcagct
     8701 ggcaggtcaa aatcagctat ttcctcctcg gtatcatctc catcgataag gtcttcgata
     8761 atgcctgcaa tatttatatc cgttatgtcc agctttgcag ccgagtcgta attgttttcg
     8821 acatggatcc ttatctctaa atctgatcca ggaaaaacgt cttttatttt tccccgtttt
     8881 gttattgatt cgccctgctt aatcccatca atatatatgt cggttatatt caccttaaga
     8941 ttattgcaga gatcttttga atttttgaac gccggctttt caggcggaac aacgatcgac
     9001 cagtcagaag caaactcggt atatccatat tttatgccga tagcttcttc ttttgttatc
     9061 ttatagtcgc ctatcccata aaagtttggc ggatcagtaa attgatcatt tttttcgctg
     9121 gttataaaga agctccagcc ataatatttg tttccgtcag ttttaggatc caaaccattc
     9181 acagacctaa gatagtacat cccagtcagc tccccatcga tcgtcatctt gatatcatcc
     9241 cttatgccat caagcttctt tagcacatca tacactgaac tgccactgcc tacatcgaca
     9301 caaccagaat aaattttgct ctcagattcg atgacaattc ccgtcttaag agcagatgct
     9361 agaggaacta gcaatataca gataaaaatg accattgtgc ttttcatctc ttcccaccta
     9421 tatgtttgtt tgaaaactga aactttacta tatctccatc ggcaagcctg tatttttttg
     9481 cgccgtaatt tatggtcttg ttgttgacat aaagcagcca caggtatttt ccatcagcgc
     9541 atatgccatc gatgcattct atgaagctgc cctcaaataa tgtatctact gaatggttgt
     9601 acttaagcag gcctaaagga gtgccgtagc taaacttaaa tctttcggtt ttgacagagc
     9661 caaggctgtc tatgacaaga gtcgcctcgc cactggccgg ctgctcttta caaccgctca
     9721 gagcgatgct tactgccaaa gcggccaata atagacttac atatctccat ctcattgaac
     9781 ttaccttttt caaatgcgaa ttttcttgcg aacggcagca cagatgtaaa agctatattg
     9841 gaaacaagat gcactgctat gaatggaaga gctgatatga aaagccctaa aatgctaaat
     9901 ggaaacatga tagcccagct aacattcata actatctcaa aaaacaaagt tgacgctatc
     9961 actactgctg taagaggaag caaagacttg agccagaacc accaatagct tttttgcggc
    10021 tttatacctt tgataaaacc tccaagaaat ccggcaaagc cccatgagag catctgaaag
    10081 atagtccagg ggccttggcc tccaaacatc aagaagtttg aaaggtatcc tgctgctgca
    10141 cctgtgatga accctttctt ctttccaaaa agccagccag caagtatcgc aaaaaatgtg
    10201 ataggctctg ctgaggggat aggctgcatg gctgccctta aggcagctcc tccaaacaca
    10261 aaccctacaa caacaagcca ctctttcatc cttaatagct gcagctgctt ttgaactgcc
    10321 tgtagagcaa ttttttgttt tagcgtcagc ttcttttcct gttttgactt taatacaagc
    10381 ttttgggaca tatgatatca gagagaatcc ttgcggacac tcctcgatcc tttactttag
    10441 caagcattcc ggctttacgg ctgcgggcca gcgattgatt tgcacaatgc tttccttgct
    10501 taattggacg aaaagtgctg gaggtataaa aagatttcta agcctttcta tatagaacga
    10561 ttacgtttat atgtatgatt aatataacgt aaggttgtat gaacttaggt agacggttgg
    10621 caatacttac aggagtcaat atgggttgca agccttgttg gccttgctgc caatcccaac
    10681 tttgacccaa acgatttaag aaatcccaat cgtcattatg ataaatgagg cagcaatcta
    10741 gggtatctac gtagcggcat ggtctattac acaagcggaa ctttgtccta tggagcaatc
    10801 ggaggttggc ttttaatcgc ttttttgaaa caatgaagtt cggggttgtc taaaaagcca
    10861 aattatttct tggaaaagga
//
LOCUS       DASRUK010000166         7745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1659530, whole genome shotgun sequence.
ACCESSION   DASRUK010000166 DASRUK010000000
VERSION     DASRUK010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 7745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7745
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659530"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1185
                     /locus_tag="VFF28_07220"
     CDS             187..1185
                     /locus_tag="VFF28_07220"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HZX45443.1"
                     /translation="MIEILWVLGLLVGGLFLLTKGSDWMTDSLVPAAERLGTTYIAVA
                     SILVSIMLSIPEIFVAVYAFYLGHSGISLGVIIGSIICNISLMTGLSAMIKPLSVDGR
                     VVIRDGVFALVISVIVFAFGYDLEFNRTEGIVLLLLFVPYAVNVWFFEKWTTKKARQE
                     ELKEIKSELNVIGLPFGELKPGLLLFIIGSAILLIGSYLFSSSLVRIAEITKLPDILI
                     GLTLGAIGPSIPNIASAIHGTIKNYTKIAITETFGADIFTLLVTLGLLAVLSPFGIDQ
                     KWLVFDIPIMILMSFIMMFFIFKGHLRGKPAILRHEGAALVFFYIVFIILNFIFLS"
     gene            1378..1755
                     /locus_tag="VFF28_07225"
     CDS             1378..1755
                     /locus_tag="VFF28_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45444.1"
                     /translation="MNAITVKCKKCGRPAKSDEFTLDLDFKMMVCAQCIKEKLMKKEV
                     YSELAKEKVVARPAGWDNDDDLLEKRYNLRLKDSVKVERIDNSKVKYTCPKCGYRFSY
                     DTDRNYPSMCTYCGEGIKKIRYD"
     gene            complement(2013..3761)
                     /locus_tag="VFF28_07230"
     CDS             complement(2013..3761)
                     /locus_tag="VFF28_07230"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HZX45445.1"
                     /translation="MDAKQLKQKYLEFFKSKGHKIIPSASLIPEHDPTVLFTTAGMHP
                     LVPYLMGQPHPLGKRLCNVQRCIRTGDIDSVGDTYHHTFFEMLGNWSLGDYWKKEAIG
                     FTFEFLTKVLEIPKERLAVTCFKGDENSPKDTEAAEAWISLGIPKERIAFLGEDNWWG
                     PAGNTGPCGPCTEVFYWKAKSGAPKLFDPEDKRWVEIGNDVLMQYNKNDKGVFEVAKQ
                     KNIDFGGGTERIIASLNSLEDNYQADVWLPIISELERTSGKKYGKDAKQTRSMRIIAD
                     HLKAAVFIIADGIIPSNTEQGYVLRRLIRRAVRHGLDIGLKDFTPKIAMPVFTIYDDS
                     PILGASRDKILTELSKEEAKFNDTLEQGIGVFEKTVRGKKELSGKDAFLLYQSYGFPF
                     EMTKELAAEKGISVDEKGFEKEAKKHQELSRTSTEGKFKSGLADHSEQTTKLHTATHL
                     LNQALREVLKKPDIFQRGSNITAERLRFDFNFDRKLTSDELKSVEVWVNQRIKENLEV
                     AKAEMTVDEAKKAGAQGVFEHKYGEKVFVYCIGDRSKEICGGPHVVNTKELGHFKILK
                     EESSAAGVRRIKAVLE"
     gene            complement(3822..4097)
                     /locus_tag="VFF28_07235"
     CDS             complement(3822..4097)
                     /locus_tag="VFF28_07235"
                     /inference="COORDINATES: protein motif:HMM:NF024636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HZX45446.1"
                     /translation="MPEITLDEYKQAYRDIEQRQEKQTFFLHLIIYAAVNLFLIAVNA
                     AYSPDAIWVVYVIFGWGIGVLMHYLFKVRWIEERLEEREARAEYKLR"
     gene            complement(4078..4377)
                     /locus_tag="VFF28_07240"
     CDS             complement(4078..4377)
                     /locus_tag="VFF28_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HZX45447.1"
                     /translation="MLSKIPLSTENMTKEDIDREIMRMGMIAELDAINLYEQLAVAAE
                     SDILKKVLMDIAREEKAHIGEFQAMLLKADPEQAKELKEGKEEVTKLEKDARDNP"
     gene            4668..5594
                     /locus_tag="VFF28_07245"
     CDS             4668..5594
                     /locus_tag="VFF28_07245"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HZX45448.1"
                     /translation="MDFKNKIILAPLAGVNNIAFRKLCTAHGADIVYSQMIDCRAMAR
                     GNNRLADFYDEKNVIAQFFGNEPESIGKCIPLIKDKVQGVDLNLGCPHSNVVERQCGS
                     YLMRFPAKISKVVKEMVKHGVPVFVKMRAGFDKSHINAVKIAKLCEKEGAAAIAVHGR
                     PRTVNYEAPVDYSIIKKVKDSVDVPVIGNGDIFCGKDAKRMYDETGCDSVMIGRGAMG
                     DPCIFDEIKAYLGGEKFDKPGKVELISEYIKYCSKYEIKFEGIKSHAQWMTKGMPGGA
                     RLRNMLNGVKDVDELIKAFATTCQEYPECKVL"
     gene            complement(5542..6285)
                     /locus_tag="VFF28_07250"
     CDS             complement(5542..6285)
                     /locus_tag="VFF28_07250"
                     /inference="COORDINATES: protein motif:HMM:NF025027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HZX45449.1"
                     /translation="MEFQKALIKLQNSIDTAIKNKVDLQSKLDSERALYNYKEKQKQY
                     YVNSVRSAVKQVKSSYLGLIEIAKDKDNQEIVNKIYELIPDLDSTDLQTLNKTLKNIL
                     SLVKLLDIKEKEKDFGTPKVPQEIYAEIKADIEELNRCFKNECYRSSVILCGRLLETA
                     LHRKYFEVTKNDLLEKSPGIGLGNLVAKLVEKRVPLDPALTNQIHLINQVRIFCVHKK
                     KEAFNPSKQQAQATILYTLDILDKLWQKP"
     gene            complement(6288..7520)
                     /locus_tag="VFF28_07255"
     CDS             complement(6288..7520)
                     /locus_tag="VFF28_07255"
                     /inference="COORDINATES: protein motif:HMM:NF016765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45450.1"
                     /translation="MNISCIKCKGATPMQSCGRSFCPILAKSQAMFRVKDGLKTDSFS
                     GSSPNVFIGHNFYPNLYVGILSPPERTADAYLYDSPKDWAAGNLQIPKIVDYRSSLIN
                     SRFRSMVKDQDKLICLSQEIGMASRPVDVEISLEEKPSFHLSTDPYLAPMGPNAKLKK
                     AEITENPHIDTRVDKVVSDSDLKANQALTYLYDKGFDENFLTKILSVGNLGIKPQRKL
                     VPTRWSITAADDAIAKHLLTGIKKHPQSDYLAYFGGYLGNYFLILFFPEVWSYELFET
                     YAPNAEWNMSDDFNYMTDSEPYDSRKTYAENCGGGYYANRLAVLEKLNHLKRQASILT
                     LRFITGEYAVPLGVWVVREASRKVLSNRPIEFSSKELMLKYAQALIKKKFGFNLETLL
                     NQSILLKNQKTQKKISNY"
BASE COUNT         2000 a         1625 c         1607 g         2513 t
ORIGIN      
        1 gtcttctcaa caacttcgta accttccatc ttcatttgaa ttggttttct catgtagtta
       61 caatgtaact acaagaacta aataaatatt tcggtgctca agtatataat tagaattagt
      121 gggataaagc taaacgatta cattaaaacg ataattttat taattggggt tttctgagga
      181 tagacaatga tagagatatt atgggttctt ggcctacttg tcggcggact cttcctgtta
      241 accaaaggtt ctgattggat gactgattct cttgtccctg ctgccgagag gcttggaaca
      301 acatatatcg ctgtagcttc aatccttgtt tcaatcatgc tcagcatacc tgagatattt
      361 gtcgcagtgt atgctttcta tcttggacat agcggaatct cccttggagt catcatcgga
      421 tcgataatat gcaatataag cctgatgacg gggctttctg cgatgataaa acccctttct
      481 gttgacggaa gagtggtgat aagagacggg gtttttgcct tggtgatatc tgtcatcgtc
      541 tttgcttttg gatatgattt ggaatttaac aggactgagg gaatcgtcct tctgctgctg
      601 tttgtcccat atgcagtgaa tgtatggttc tttgagaaat ggacgacaaa gaaagcaagg
      661 caggaggagc tcaaagagat caaaagcgag cttaatgtga tagggctgcc atttggcgag
      721 cttaagcccg ggcttttact ctttataata ggctcagcaa tactcctgat aggatcctac
      781 cttttttcct cgtcccttgt aaggatagcg gagataacaa aactcccaga catacttatc
      841 ggacttaccc ttggagctat cgggccttcc atcccaaaca tagcctctgc aatccatgga
      901 acgataaaga actatacaaa aatcgccata actgaaacat ttggggctga tatctttact
      961 ttgcttgtga cacttgggct tcttgctgta ctgagcccat ttggcataga ccaaaaatgg
     1021 cttgtatttg acataccgat aatgatattg atgagcttta tcatgatgtt tttcatcttt
     1081 aaagggcatc ttagaggcaa gcctgccata cttaggcatg aaggggcagc tctagtattt
     1141 ttttacattg tctttatcat ccttaatttt atattccttt cctgatcatc tgctaactga
     1201 attataaaac aaaaaagcct atggtttttg tcgtcaggtg gaatcttttg ctcaaacaca
     1261 ctcattatag agtcaaaaaa cacttgctgt tggctaccag atatgctacc tacctttgat
     1321 gggcggttaa cacaattaaa aaaatttata tatgcaggca gagaatatgt tttttctatg
     1381 aacgctatca ctgtaaaatg caagaagtgc ggaaggcctg ctaagtctga tgaatttacg
     1441 ctcgatcttg attttaagat gatggtatgc gcacagtgca tcaaggaaaa actgatgaaa
     1501 aaagaagtct attctgaact tgcaaaagag aaagttgtgg caaggccggc gggctgggat
     1561 aatgatgacg accttcttga gaaaaggtat aacctgcggc taaaagattc tgtaaaggtc
     1621 gagaggatag ataactcaaa agtcaagtat acatgcccga aatgcggcta tcgcttttcc
     1681 tatgatactg acaggaacta tccttccatg tgcacatact gcggggaagg gataaagaag
     1741 ataaggtatg attaaatttt ccttacggct cactgatcat accaatctgc ttttgtttct
     1801 tctcaaattc ttttgcaagg aggaggtcag tcaacctgcc tatcgcctga gttctactac
     1861 agtatatctc ttcaagatag taggctgatc ttacctcaga aggataataa actaattctt
     1921 cctgaattga cccccaataa aggaaattac atccctccta tcaataaatc catcgaacat
     1981 atatcgatag aagtgaaaga ttgcttaaaa agttattcca acacagcctt tatccttctc
     2041 acacctgctg ctgagctttc ttcctttagt atcttgaaat ggcctagctc ttttgtattt
     2101 acgacgtgag ggccgccgca tatctctttt gagcggtctc caatacaata gacaaagact
     2161 ttttctccat acttgtgctc aaagacgcct tgggctcctg ctttttttgc ctcatcaact
     2221 gtcatctctg cctttgcgac ctctaggttt tccttaattc tctggttcac ccaaacttcg
     2281 actgatttta gttcatcact ggtcagcttt ctgtcaaagt taaagtcaaa ccttaatctc
     2341 tctgctgtga tatttgatcc tctttggaat atatccggct tctttaaaac ctctcttaga
     2401 gcctggttta agagatgggt tgctgtgtgg agctttgttg tctgctctga atgatctgca
     2461 aggcctgatt tgaattttcc ttctgtagag gtcctgctta gttcctgatg cttttttgcc
     2521 tctttttcaa accctttttc atccactgag atgccctttt ctgctgcaag ctcttttgtc
     2581 atctcgaatg gaaatccata gctttgatac agcaggaagg catcttttcc tgacagttct
     2641 tttttgcctc ttactgtctt ttcaaatacg cctataccct gctctaaggt atcgttaaac
     2701 tttgcctctt ctttagaaag ctctgtcaag atcttatccc tacttgcccc tagaatcgga
     2761 ctatcgtcat atatcgtaaa taccggcatc gctatctttg gcgtgaaatc cttcaaacca
     2821 atatccagcc catgccttac agccctcctt atcaaccttc ttagaacata tccctgctcg
     2881 gtatttgacg ggatgatccc atcagctatt ataaatacgg ctgctttgag atgatctgct
     2941 attatcctca tgcttcttgt ttgctttgcg tccttgccat attttttgcc ggaggttctt
     3001 tcaagctcgg atattatagg aagccaaaca tcagcctgat aattatcctc aaggctgttt
     3061 aatgaagcaa tgatcctttc tgtgcctcct ccaaaatcaa tattcttctg ttttgcaacc
     3121 tcaaaaacgc ccttatcatt cttgttatat tgcataagca catcattgcc tatctctacc
     3181 catcttttat cctcaggatc aaaaagcttg ggcgctccag attttgcctt ccaatagaat
     3241 acctctgtgc atgggccgca tgggcccgtg tttcctgctg gtccccacca gttgtcctct
     3301 cccaggaatg ctatcctttc ttttgggata cctaggctta tccatgcttc tgctgcttct
     3361 gtatcctttg gcgagttttc atcaccttta aagcaggtca ctgccagcct ttcttttgga
     3421 atctccaaaa cttttgtcag gaattcaaag gtaaatccaa tcgcctcctt tttccagtaa
     3481 tcgccaaggc tccagttgcc aagcatctca aaaaaggtat gatgataggt gtctcctacg
     3541 ctatcgatat ctcctgtcct tatgcatctt tgaacgttgc atagcctttt gccaagagga
     3601 tgaggctggc ccatgaggta ggggaccaaa ggatgcatgc ctgctgtggt gaataggaca
     3661 gtcgggtcat gctctggtat caacgaagca gagggtataa tcttatggcc tttacttttg
     3721 aaaaattcaa ggtacttttg cttaagctgt tttgcgtcca taacctggac aagtcctatc
     3781 tcttttaaaa aaattgcggt ttaaactggg attatttgct tctagcgcaa cttatactct
     3841 gcccttgctt ctctttcttc aagcctttcc tctatccagc gaaccttgaa caagtaatgc
     3901 atcaggacac cgatgcccca tccgaagatg acatagacaa cccaaatagc gtctggtgaa
     3961 taggctgcat tgactgctat aagaaagaga tttactgcgg catagatgat aagatgaagg
     4021 aaaaatgtct gcttttcttg cctttgctct atatccctgt atgcctgctt gtattcatca
     4081 agggttatct ctggcatctt tctcaagttt tgtcacttcc tcctttcctt cttttagctc
     4141 ttttgcctgc tctgggtctg cttttaagag catcgcctga aactccccaa tgtgggcttt
     4201 ttcttctctt gctatgtcca tgaggacttt ttttagaata tcactttctg ctgcgactgc
     4261 aagctgctca tagaggttga tggcatcaag ctctgcaatc atgcccatcc tcattatctc
     4321 cctatctata tcctcttttg tcatattctc tgtacttaac ggtatctttg acaacatata
     4381 gaacaccccc tgccaataga caggtgtttt tttgatattt aaggttaccg aatactaggc
     4441 ttttgtattt ttagttttgc taaaatggct aaaagctcaa tatttcgcct atatggctcc
     4501 tgagagccta ttttcagttc tgttttctag tgtttttgct tgtcgtcgat aagtgcaaaa
     4561 gtctagaacg tgattgccat actgatgtga cctatggtgc ctgcttgatg aatctagcag
     4621 ataacgagcg gaaaagtaat taaataggat atggtttttc catttatatg gattttaaaa
     4681 ataagataat acttgcccca cttgcaggcg tcaacaacat cgcattcaga aagctctgca
     4741 ctgcgcatgg agctgatatt gtttattcac agatgatcga ctgcagggct atggcgaggg
     4801 ggaataaccg gcttgctgac ttttatgatg agaagaatgt gatagcacag ttttttggca
     4861 atgagcctga atctatcgga aaatgcatac cattgataaa ggataaagtg caaggtgttg
     4921 acttaaacct tggatgcccc cacagcaatg tcgtcgaaag gcaatgtgga agctacctta
     4981 tgagatttcc tgcaaagata agcaaggtag taaaagagat ggtcaagcat ggggtgcctg
     5041 tgtttgtgaa gatgagagct ggatttgata aaagccatat aaatgcagta aagatagcaa
     5101 agctctgcga gaaggaaggt gcagctgcta tcgctgtcca tggaaggcct agaactgtca
     5161 attatgaagc gcctgtcgat tactccatca taaagaaggt caaggactct gttgatgttc
     5221 ctgtgatagg aaatggagac atattctgcg gtaaagatgc aaagaggatg tatgatgaga
     5281 caggatgcga ttctgtcatg ataggaagag gggctatggg tgacccttgt atatttgatg
     5341 agataaaagc atatcttggc ggagagaagt ttgacaagcc gggcaaggtt gaacttattt
     5401 ctgagtacat caagtattgc tcaaaatatg agataaaatt tgaaggcata aaaagccatg
     5461 cgcaatggat gaccaagggc atgcctggag gagcaaggct tcgcaatatg ctaaacggcg
     5521 taaaagatgt tgacgagctg atcaaggctt ttgccacaac ttgtcaagaa tatccagagt
     5581 gtaaagtatt gtagcctggg cctgctgctt tgatgggtta aacgcttctt ttttcttgtg
     5641 aacacagaat atccttacct ggttgataag atgtatctgg tttgtgagcg caggatcaag
     5701 cggtactctt ttttccacaa gctttgccac aagatttcca aggcctattc ctggggattt
     5761 ttcaagcaag tcattttttg tgacctcaaa atatttccta tgaagggctg tctcaaggag
     5821 ccttccgcaa aggatgactg agcttctgta gcactcgttc ttgaagcagc ggtttagctc
     5881 ttcaatgtct gcttttatct ccgcatagat ctcctggggg acttttggtg tgccaaaatc
     5941 tttttccttt tcctttatat ccagcagttt tacaagcgaa agaatgttct ttaaggtttt
     6001 atttagggtt tgcagatcag tcgaatccag atcagggata agctcatata tcttgttgac
     6061 tatctcctgg ttgtccttat cttttgcaat ctctatcaga ccaagatagc tggatttgac
     6121 ctgctttact gctgacctca ctgagttaac ataatattgc ttttgctttt ccttataatt
     6181 ataaagggct ctttccgagt ccagttttga ttgcagatca accttgtttt ttatcgctgt
     6241 atctatcgaa ttctgaagtt ttatgagcgc tttttggaat tccatattta gtagttggaa
     6301 atttttttct gggttttttg gttttttaac aggatgctct ggtttaataa ggtttccagg
     6361 ttgaagccaa acttcttctt tatgagagct tgagcatatt taagcatgag ttctttgctt
     6421 gaaaactcga taggcctatt gcttagcact tttcttgagg cttccctaac tacccagaca
     6481 ccaagaggga ctgcgtattc tccagttata aacctaaggg taaggatgct tgcctggcgc
     6541 tttagatggt ttagtttttc taggactgcc aagcggtttg cataatagcc tccgccgcag
     6601 ttttctgcat aggttttccg gctatcatag ggctcactat ctgtcatgta gttgaaatcg
     6661 tctgacatat tccattctgc gtttggggca taggtttcga ataattcgta agaccagact
     6721 tctggaaaga aaaggatgag aaagtaattt cccaggtagc cgccaaaata ggcgaggtaa
     6781 tctgactgcg ggtgcttttt tattccagtt aacagatgct ttgctatcgc gtcgtctgcc
     6841 gctgttatag accatcttgt gggcacgagc ttcctttgcg gctttatgcc gagattacct
     6901 acgcttaata tctttgtgag gaagttttca tcaaaacctt tatcgtagag gtaggtcaga
     6961 gcttgatttg ccttcaggtc gctgtctgat actactttgt caacccttgt gtctatatgg
     7021 gggttttctg ttatctctgc tttctttagc tttgcatttg ggcccatggg agcaagataa
     7081 gggtctgtgc ttagatgaaa agagggtttt tcttcaaggc ttatctctac atcaaccggc
     7141 cttgatgcca tgcctatctc ttgcgacagg cagataagct tgtcttggtc ttttaccatc
     7201 gagcgaaacc ttgagtttat gagagaagag cgataatcta caatctttgg aatctgcagg
     7261 ttacctgcag cccagtcctt tggagaatca tagagatagg catctgctgt cctttccggc
     7321 gggcttagta tacccacata gaggtttgga taaaagttat ggccgataaa cacatttggg
     7381 gagctgcctg aaaaactgtc tgtttttaat ccgtctttta ccctgaacat cgcctgcgac
     7441 tttgcaagta taggacaaaa ggagcggccg catgactgca tgggcgttgc acctttgcac
     7501 tttatgcaag agatgttcat ttaaaacaga gaaggattta gattaaaaaa gtttgtcatt
     7561 tagcccaggc atatcttggc attagacgag tttaggccat tttagtaagt attcagtcag
     7621 ctagcaaatg aaatcagatt ttcatagaca tttttccctt gctgattcca tgtttccatt
     7681 gccgacatca catttccaat aaaccttctg ccattttcat ttcttatgca gcccatgagc
     7741 tttct
//
LOCUS       DASRUK010000167         4646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2313040, whole genome shotgun sequence.
ACCESSION   DASRUK010000167 DASRUK010000000
VERSION     DASRUK010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2313040"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..4328)
                     /locus_tag="VFF28_07260"
     CDS             complement(78..4328)
                     /locus_tag="VFF28_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HZX45451.1"
                     /translation="MERSLGICIGASSIKAVEIDDKGNVLSSTIKNHECNPRKEFKEL
                     LNKIKLSDYNYVALTGRKFKDLVNLPNITEPEATEYALFQYLQESGKKFDALVSLGSE
                     NFMLYELDDLGHILNVRTGNKCASGTGEFFLQQIRRMDLSTDEAIKQANVSEPHRVSG
                     RCSVFCKSDCTHALNIGVPAGKISAGLGDMIAAKVLELTGKKENIIGVGGVTRNSYVA
                     RRLSEKIKNFFIPESAPYFEALGAAIYAMKNKVAFKDELMFSDEADSFTRLPDLKKYT
                     HMVEFKEKKHGKALEGDECILGLDVGSTTTKAVLLRVSDDSVLASVYLRTNGNPVKAS
                     RECYKEINKQLSGINVSIAGLGVTGSGRKIAGLHGLTDGVINEIIAHATGATYFDKDV
                     DTIIEIGGQDAKYTYLVNGVACDYAMNEACSAGTGSFLEEASKESLNINYLDIQDIAL
                     QGKNPPNFNDQCAAFISSDIKNASHENIGRDDIVAGLIYSICMNYSNRVKGSRKIGEK
                     VFMQGGVCYNKAVPLAMAGIINKPIVVPPDPGLIGAFGVALEVKNRINNGLLKKSSFD
                     LIELTNREVAYEKPFICPGKPENCDRGCLINVIKINGKNYPFGGICNKYYNMMHNISV
                     DASHLNLVEKRQKMYFKEEKNEGAKTVGIIRSFYVNMLYPLYFGFFNKLGLKVILSDK
                     ADPEGMLMTASSFCFPAQIAHGMFMDLVKKKPDYIFMPHVTELYVKGSKMEGPERHCA
                     CLIGQSETYYIQSTFRDVSAKIISPVLNFSKGWGTMEEQFVKVGVDLGVTPEKAKQAF
                     LSGVMRLNEVNSFKKKMGEEVLSQLAADKSKIGIVLFGRAYNAFADEANLGIPGKFSS
                     RGVYIIPFDCLAFEDEDNIENMNWAVGQDIVKAAKLVKRHPQLFGAYITNFSCGPDSF
                     LIGYFRDIMGTKPSLTLEIDSHSADAGINTRIEAFLDIVDRFRKLGLKEIPPHSYTPA
                     EVVAEKGSLKFIASDGTKYDATDPRVKMIIPSMGRTTSEAGAAFFRGLGIDASAVKLP
                     DFSVLMVGRGNTSCKECLPLILTTASLLDHIQNRKKTDELLIYFMPTVTGNCRFSQYY
                     VFLKRLVEKKRIENVAFFSLNSTKNYAGLGPLDQLGLLKGIMIADIMDDIKNALLVLP
                     KDKKEAMKVFETQWQKIIRSLETGGKDIYGVLKDVSLELSKIQLRFNLASAKKVLVAG
                     EIYVRKDEFSSQEVIKRLSKRDIVCKRSPVIEWIYYCDYILKNIKSTRIGLRDNAEIF
                     LRRIVQNKLERKIKKILCKSGLYEYEEVDIAKVMEVGSHIINPLLTGETIVVAGGFFH
                     EIGSKAHGMVSIGPFGCMPTRVSEAILSNECKIAGNKRLDGLENIHILKQLNTLPFLS
                     VEADGNPFPQIVEARIEAFALQVERVYEKMVSIQSASK"
BASE COUNT         1195 a         1120 c          897 g         1434 t
ORIGIN      
        1 gaaggtttat tggaaatgtg atgtcggcaa tggaaacatg gaatcagcaa gggaaaaatg
       61 tctatgaaaa tctgatttca tttgctagct gactgaatac ttaccatctt ctcatacacc
      121 ctctccacct gtagcgcaaa agcttctatc ctggcttcca ctatctgcgg gaaaggattg
      181 ccgtctgctt cgactgaaag gaaaggtaac gtattcagct gctttaggat atggatgttt
      241 tcaaggccat caagccgttt gttgccggct atcttgcatt cgttgctgag tatcgcctcc
      301 gacactcttg tgggcataca tccaaacggg ccgattgata ccatgccatg ggccttgctt
      361 cctatctcat ggaaaaatcc tccggcgacg actattgttt ctcctgtaag aagaggattg
      421 attatatggc tgccgacctc cataaccttg gctatgtcga cctcctcata ttcataaagc
      481 cctgacttgc ataatatctt cttgattttc ctctcgagct tgttttggac tatacggcgc
      541 aggaatatct cagcattgtc cctaagtcct atcctggtgc tttttatatt tttaaggata
      601 tagtcgcaat aatatatcca ctcaatgaca ggcgaccttt tgcagactat gtctctcttc
      661 gaaagtcttt ttatgacttc ttgagaagaa aactcatcct ttcggacata tatctccccg
      721 gcaacaagca ctttctttgc agaagctaga ttaaacctta attggatttt cgagagctca
      781 agcgaaacat ctttaagtac accataaatg tcctttccgc cggtctcaag tgatctgatt
      841 atcttttgcc attgggtctc aaagactttc atggcttctt tcttatcctt aggaagcaca
      901 aggagtgcat tcttgatgtc atccattatg tctgcgatca ttattccttt aaggagtccc
      961 agttggtcaa gcggtccaag cccagcataa ttctttgttg aattcaaaga aaagaacgca
     1021 acattttcga tccttttctt ctcaacaagc cttttcagga atacatagta ttgtgaaaac
     1081 ctgcagtttc cagtcaccgt tggcatgaaa tatataagca gttcatcagt tttttttctg
     1141 ttctgtatat ggtcgagaag gcttgcagtc gtgaggataa gtggcaaaca ttccttgcat
     1201 gaagtgttcc ctctgcccac cataagcacg ctaaagtcag ggagttttac agcagatgca
     1261 tctatcccaa ggcctctaaa gaaagcagct ccagcctcag atgttgtcct tcccatcgag
     1321 ggtattatca tcttgaccct gggatcggtt gcatcatact tggttccatc gcttgcaata
     1381 aacttaaggc ttcctttttc tgcgactacc tctgcaggag tataactgtg aggagggatt
     1441 tcctttaatc ctagcttcct gaatctgtcg acgatatcta aaaatgcttc tatccttgtg
     1501 tttatccctg catcagcaga atggctgtct atctcaagtg taagtgaagg ctttgtgccc
     1561 atgatatccc tgaaataccc tatcaagaaa ctatcaggcc cgcagctgaa gttggttatg
     1621 tatgcaccaa acaattgcgg gtgtctcttg acaagctttg cagccttgac aatgtcttgt
     1681 ccgacagccc agttcatgtt ctctatgtta tcttcatcct cgaaagccag gcagtcaaag
     1741 ggtattatat agactcctct tgaagagaat ttaccaggaa tacccaggtt tgcttcgtca
     1801 gcaaatgcat tgtacgctct tccaaaaagc acgatcccta tcttggattt atcagctgca
     1861 agctgggaaa gcacttcctc ccccatcttt ttcttgaaag aattaacctc attaagcctc
     1921 atcactccag acaagaatgc ttgcttagcc ttttcaggtg taactccaag gtccactccg
     1981 acctttacaa actgctcttc catagtcccc catcccttgc tgaagttgag cacagggctg
     2041 attatcttag cagagacatc acggaacgtg ctctgtatgt aataagtctc agactgtccg
     2101 atcaagcatg cacagtgtct ttctggccct tccatcttgc ttccttttac ataaagctca
     2161 gtgacatgtg gcatgaatat ataatcaggc tttttcttta caagatccat gaacatacca
     2221 tgcgctatct gtgctggaaa gcaaaaagac gatgcagtca tcagcatccc ttcaggatca
     2281 gccttgtcag ataagattac cttaagccca agcttgttga agaatccaaa ataaagggga
     2341 tagagcatat tcacataaaa gcttcttatt attcctactg ttttagcgcc ttcattcttc
     2401 tcttctttaa aatacatctt ctggcgcttc tcgaccaggt tcaagtggga agcatcgaca
     2461 gagatattat gcatcatatt atagtacttg ttgcatatcc cgccgaaagg atagtttttg
     2521 ccgttgatct ttattacatt tatcaagcaa cctctgtcac agttctcagg ctttccaggg
     2581 cagatgaagg gtttttcata tgctacttca cgattggtaa gttcgataag gtcaaagctt
     2641 gattttttaa gaagcccgtt gtttatcctg ttctttactt caagtgcaac tccaaaagct
     2701 ccgataagcc cagggtcagg cggcacgact atcggcttgt ttattatgcc tgccatcgca
     2761 agaggtacag ctttgttata acatactcct ccttgcatga aaactttttc tcctattttt
     2821 cttgagccct ttaccctgtt gctgtagttc atgcatatag aatatatcaa gccagcgacg
     2881 atatcatctc tgcctatatt ctcatgtgaa gcatttttta tgtcagagga gatgaatgca
     2941 gcacattggt cgttaaagtt tggaggattc ttcccctgta gcgcgatatc ttgtatatcc
     3001 agatagttga tgttaaggct ttcctttgag gcctcctcaa gaaagcttcc tgtccctgca
     3061 gagcaggcct cgttcatagc atagtcgcag gcaacgccat tgaccagata agtatatttt
     3121 gcatcctggc ctcctatctc gattatggtg tcgacatcct tgtcaaaata tgtagctcct
     3181 gtcgcatggg cgattatctc attgattact ccatcagtca gaccgtgaag tcctgctatc
     3241 tttctgccag agcctgtcac tccaagccca gctatactga catttatacc tgagagctgt
     3301 ttgtttatct ccttataaca ctcacgtgaa gcttttacag ggtttccatt tgtcctcaga
     3361 tacacagagg caaggacgct gtcatcagag actctaagta ggacagcctt tgttgttgta
     3421 gagccgacgt caagtcccag gatgcattca tcgccctcta aagccttccc atgtttcttc
     3481 tccttgaact cgaccatgtg agtgtacttc ttaaggtcag gaagccttgt aaagctgtca
     3541 gcttcatcgc taaacatcag ctcgtctttg aaagcgacct tgttcttcat ggcatagatt
     3601 gcagccccaa gagcctcaaa ataaggagcg ctttcaggaa taaaaaaatt ctttatcttt
     3661 tcagaaagcc ttcttgccac atagctgttt ctagtgactc cgcccactcc gatgatattt
     3721 tccttcttgc ctgtaagctc aaggactttt gcagctatca tgtccccaag ccctgcgctt
     3781 atctttcctg caggaacacc gatgtttagg gcatgtgtgc agtcgctctt gcaaaacaca
     3841 gagcaccttc cagatacacg gtgcggctca gacacatttg cctgctttat agcctcatca
     3901 gtactgaggt ccatccttct tatctgctgc aaaaagaact cgccggtgcc agaagcgcat
     3961 ttgtttccag tcctgacatt aaggatatgg ccaagatcat ccagctcata aagcatgaag
     4021 ttttcgcttc caaggctgac caaggcatca aacttctttc cgctctcttg caggtattgg
     4081 aaaagcgcat actcagtggc ctcaggctct gttatgttag gcagatttac aagatcctta
     4141 aatttcctgc cggtaagagc aacataatta tagtcagaaa gctttatctt gttaagcagt
     4201 tccttaaact cttttctcgg gttgcattca tggttcttta tggtgctcga aagaacattg
     4261 cctttatcgt cgatttctac tgcctttatg cttgaagccc cgatacaaat accaagagat
     4321 ctctccatca gaaaggggat aagtcagcgc tttaaaatgt tttctatagc gtaaaaaacc
     4381 gagtgcatgt aatcccagaa ggtgaaattt tgtaaagccc aacaacaaat aatgcctttc
     4441 tgatgttaag atgatctacc ttatttcgcc tatactcact taacttcaac caacatcgcc
     4501 tgccacttcc cgcacctatc gttccatctg gaagatatct cgccattaac ataggattca
     4561 tgcacttcgc aaacattagg gcagtctttg cagacgaatg ttttagtaaa cgtttgttca
     4621 ttgatgtcaa agcctcgaaa ctttgt
//
LOCUS       DASRUK010000168        11022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2471824, whole genome shotgun sequence.
ACCESSION   DASRUK010000168 DASRUK010000000
VERSION     DASRUK010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 11022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11022
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2471824"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..1102
                     /locus_tag="VFF28_07265"
     CDS             299..1102
                     /locus_tag="VFF28_07265"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZX45452.1"
                     /translation="MKVQIENKINPNIELYRKEDLDISYQFAKDLHAEFGTFIKACVL
                     FGSSARKKRKPESDLDIMVVVDDLSINFSGELVEAYRVIVQKLIAKNSTRLHITSLRF
                     SSFWEYIRNGDPVAINILRDGVALIDTGFFEPLQILLRRGRIRPTEESIWNYYVRAPN
                     TLHNAKWHILQGTIDLYWAVIDAAHAALMKHGEIPPSPEHVADLLQEKLVKHRLLEQR
                     YVTMMRNFYKLSKMITHNEISELKGEEFDRYYKDAAEFVDRMRRYIGWK"
     gene            complement(1103..1897)
                     /locus_tag="VFF28_07270"
     CDS             complement(1103..1897)
                     /locus_tag="VFF28_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010504813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HZX45453.1"
                     /translation="MAEFLQALIMAFREGLEAFLIIAILLKFLDKTNNKGLKKSVWQG
                     TAFGVIASLALGLLLAYASAIFSGASKLWESAASLIAVLLVTTFIIWMINHGSKIKEH
                     VESKASLSLSKKGIILLALFMVAREGAEIAIFSFAGDYPVLPIMLGVLGSIVLVLAIY
                     HSFVKINLKILFSITLVYLILQAGFLIGYSIHEGLSAAKGIVGENSPLYAKAFDLSGT
                     ALDHKGGFLGMPVYIAFGWYSKPEWIQFLAQYGYTLILLGYWWKKR"
     gene            complement(1899..2297)
                     /locus_tag="VFF28_07275"
     CDS             complement(1899..2297)
                     /locus_tag="VFF28_07275"
                     /inference="COORDINATES: protein motif:HMM:NF014762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HZX45454.1"
                     /translation="MHKETAENYLRAIYHIQEEKGTVKSIDLCRYLHIGKSSISEMIR
                     NLAEKNLVCLNPYSTIRLTKKGLSRSMEITRKHRLIEAFLSEFLGIKSSLHEEAHRLE
                     HAFSEESIGSIRKMVADSKHCPHGKPIPGG"
     gene            2536..3696
                     /locus_tag="VFF28_07280"
     CDS             2536..3696
                     /locus_tag="VFF28_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007044428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HZX45455.1"
                     /translation="MVGKEGVKKGSSKEIHLTERLKLTSNERVKELEDEIAKTKYNKR
                     TQRAVGLMKAKLAMLKDRASQRSGGGKGDDRFSVRKTGDGTAVLLGFPSVGKSTLLNK
                     VTNAKSDVAAYSFTTLKAVPGLMKYGHAKIQIIDVPGIVSGAAAGRGRGKEVLQMLRN
                     ADLILILIDALYPEHYPALLEEIKETGIRVNEQKPRVNIVKKDRGGISISETVPLTKI
                     DYETISGIAREMRLNNCQITIHTDIDSDQLIDVISADKVYTKSLAVLTKIDLLSDEQL
                     KELKARVKPDVCISAEAGIGIEDLKKAIYDKMRFMRVFLKEVNKKPDLEEPLIMFKGA
                     TLRDVCSKLHKDFVEKFNHARLWGKSAKFEGQAFRKLDKELCDGDIVELHIR"
     gene            4125..4781
                     /locus_tag="VFF28_07285"
     CDS             4125..4781
                     /locus_tag="VFF28_07285"
                     /inference="COORDINATES: protein motif:HMM:NF014706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing 3 family protein"
                     /protein_id="HZX45456.1"
                     /translation="MFDKQKQDCLGKVDKSNKQSIDKGIKRLVDILNKSRDYYTTSSC
                     AGRIVLIAKKSYKKSEMRFVHSTHSKASFSKIKKDLLQAYPSGRVDGNVGKRSSSQYQ
                     GYPQGGRKGAVSGYIGSKSDIWLRQESFILHVCCRTQEAAAKLLNMARDLGIKRAGII
                     YLGKRIMVEIIGTESMETIIAKKGKLLVTDDYLKLLLDEANKKLKRNCKKLEELYLSL
                     "
     gene            complement(4770..8693)
                     /locus_tag="VFF28_07290"
     CDS             complement(4770..8693)
                     /locus_tag="VFF28_07290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45457.1"
                     /translation="MNDGCTGPIKFAPNEQHGLHVCAVDVLNKTYGCKAYNFGEYGAG
                     DSLETDAFQMNSSSYRLKFNWARIRDPNGRYDHGGISAQSLWQCTHDPFALRSYSNPD
                     CSWPPGRWISGYWSSTDGSCTGTYIESKRCKNYPSTCRNYENNSIGGSDCILGDFAAQ
                     GCSGPSDTYEATNSPGDCSGDQQYYQSYYSHPTSCIDGTPSNCPANLCTFAGNTTVYT
                     AAAAKGIVIKIEDKIKAIGYATFNNCNCTLSECCSDGCHYDPKGTACGESRLCDNGGV
                     CISTRKQPKITKTTYSYDPYGNIIKIYHEGDVLKTGDEKTIINSYDSDANRWILNKLN
                     QTSLLSYDNSTVRQTEYSYDAKGDLIQRKDWSDGGEDPTMRFFYDSYGNLIESIDANG
                     HSTRYEYDATYTLVTREIDASNNSAIYTYDNLGRLTSQADPNGFTTHHVYDTFGRRVK
                     QIRPYDTMENPTLQITYNLDGIAPEEAITRQREEYSDTIDTYQYYDGFGRIVQQKAGR
                     GLFISQDISYDARGRQKSFSNAYYSSQGFSPASNVVGTSYLYDIMCRVILQQSPDGTK
                     IESSYDHWSKQITDKGGIKKAFIYDAYGNIVFVKEYNDGDTYTTSYEYDTLGNLISLT
                     DSQANKITYTYDALGRKTRTNDSDLGIWSYTYDKAGNLIVQEDNKGNAIELQYDNTNR
                     LLSRKTVDQEIIYTYDAITKGTLSSVKSKDMIKKYWYDERLREVKEEIIIDGISFNTT
                     FQYDSYDRLKRAVLPNGEALSYGFNEQGLVRSVDGILDNIEYNEFGQPKKRLYSNGLK
                     TDFAYGNDLRISHIKTGDMQDYTFNYDPAGNLITLKDLIANQSDEFAYDDLERLKVAN
                     VGGKDIQYEYDLIGNMKKAIGGNRSLHFAYAERVHAPEAVIINQSCQADLVSEYSDWG
                     NIICLSNDRMNQSRSKKTYDANYCGEVANSTTIEYRAIEACDYCTPNLLEVYSDWQNI
                     SCHPNDKMNQTRTKITYDAHFCDEVSNITIIEYRATEACDYCTPDIQTRYTQWVNLQC
                     LPEDLLNQSRVAIAYDANYCGEAENTTTVEYQSIENCDYCIPNMIESPWSDWYNTSTC
                     LIMDRITQARKKTLYDSNLCNEIANETSFEYMNSSCNYCTSAWSSYNTTCSEDSYRTI
                     FTYSNSCCADTSLASDCNIPDNATYPCQYSFNISLRKGWNLVSIPLDVDKPLESIAGK
                     FSAIWGYSGAEWSNLLSVNETIGFWINMDEESILTVVGQRVNNVSYTLFGLVGYPNLT
                     GTDVSDLYNNPVYAYNGSWQSYIPGKEYNSLQTLKPGYGYWVLER"
     gene            complement(8803..>11022)
                     /locus_tag="VFF28_07295"
     CDS             complement(8803..>11022)
                     /locus_tag="VFF28_07295"
                     /inference="COORDINATES: protein motif:HMM:NF023677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HZX45458.1"
                     /translation="NSNLLRKYLFSYSDLGHITGSFLTSIQEAGADGTTKLPATTFGY
                     NTPSAGWEDDAAWQLPQGANFGKDGWKDNGVRLVDINFDGLTDILRMYNSSDLELWIN
                     NGEGWEQKKTIGSFFAGGFVGSDGLDKGVRFFDLDSDLRLDIIKAEAGERVVKINRGG
                     NEGWQWNTSNISFPQEADFIKTTGRIIQYIDCNPGGCPSGYSDIGYICDGDYCNITCS
                     NRQCAGSEPQLIWQDTETDSGYGGWCEAKTDYFYYCGSSGGDCIQPQPVCFVLQHLGG
                     GGGADSSVRSQAYEMSCGGSGYDGCTDEFDGSWEWRYEKIMYCVPNFLSCGGDSSCRN
                     DCWKPGAKSWTYWELDPSPNEDCTDSVTFQANEQCGAGLYVYTVPVEGEEIVKSCERQ
                     KVYQLPMDQGVNVFDVNGDGHSDIVKSTALEKKTWTSDGKRWSLSPEWQMPEDFVDEN
                     GTDKGVRIADLNGDGLPDILKANGTATKSWLNSGKGWIPNDIWTVPTEAMFLSGGNDT
                     GVRIEDINADGLFDLIKNNGSSSKTWLNNGAGWSLVNEWALPAFASTINSLVRLADID
                     GDGSLDIIKEDGVLINKATYPFLRVSNNSHGGTTTINYTKISNFDNDGDDGISDFGFA
                     GWVVQSISQDSGMAGRWKAVTLIKYNYTGAYYSSSEREYRGFGKVTEKLADVEIEHYY
                     HQDDGRASLEYKTDTFDLQGNPFKSVVYNWQSKEKQGCYVTTLDDITEYTYDGLSWD"
BASE COUNT         3068 a         2417 c         2267 g         3270 t
ORIGIN      
        1 cagaaagatt tacaggaaaa ttaatggtaa gttaagtgaa gggaaggagc agttttttgg
       61 cattgaagcg attgagacag gaaatcccac tggtatgatt ccttttccaa gaaataattt
      121 ggctttttag acaaccccaa tttcattgtg taaaggcaaa gaagagccaa gataatttag
      181 ccaccaatat caaaactaag tttaccgctg gcagatattt gcataagctt gaaggtacac
      241 cattccataa tagtcataaa cctttaaata actacttagg actaaaagcc tttatcccat
      301 gaaggttcag atagagaata aaataaaccc caatatagag ctctatagaa aggaggatct
      361 tgacatatca taccagtttg caaaagatct ccatgcagaa ttcggcactt tcataaaggc
      421 atgtgtgctt tttgggtctt cagcaaggaa gaaacgaaag ccagagtcag acctcgacat
      481 catggtggtt gtcgacgacc tgtcgataaa cttctcagga gagcttgttg aggcttatag
      541 ggtcattgtc caaaagctta tcgccaagaa ctcgacaagg cttcatataa catcactaag
      601 gttcagctca ttttgggaat atattcgaaa cggcgatcct gtcgcgataa acatacttag
      661 ggatggtgtc gcccttatag atacaggatt ttttgagccg ctgcagatac tgttaaggag
      721 gggaaggatc aggcccacag aagaaagcat ctggaattat tatgtgagag ccccaaacac
      781 tcttcacaac gcaaaatggc atatcctcca ggggactatc gatttgtatt gggcagtgat
      841 cgatgcagca catgcagcct tgatgaagca cggagagata ccaccttcgc ctgagcatgt
      901 cgctgatctt cttcaggaaa agcttgtcaa gcataggctc cttgagcaaa ggtatgtgac
      961 catgatgaga aatttctata aattgtcaaa gatgatcacc cataatgaga tttctgagct
     1021 aaaaggagag gaatttgacc gctattataa ggatgctgcg gaatttgtgg ataggatgag
     1081 aagatatatc ggatggaaat aactatcttt tcttccacca atagccaagc agtatgagtg
     1141 tatagccata ctgtgccaag aactgtatcc actcaggctt tgaataccat ccaaaagcta
     1201 tatacacagg catccctaaa aatccgcctt tatggtcaag ggctgttcca gacaggtcaa
     1261 atgctttggc atacaaaggg ctattctctc ccactatccc ttttgcagca gacagccctt
     1321 catgtatact atagcctatc aaaaaaccag cctggagtat cagatagaca agggttatgc
     1381 taaataatat tttcaggttt atcttgacaa aggaatggta tattgcaagc accaggacta
     1441 tagagcccaa aactccaagc attataggaa gtacaggata gtctcctgca aatgaaaata
     1501 tcgctatctc tgccccttcc cttgctacca taaacaaggc aagtaagatt atccctttct
     1561 ttgacaatga aagcgatgcc ttgctttcta catgctcttt tatcttgctg ccatggttta
     1621 tcatccatat gatgaatgtc gtgacaagta acactgcaat caagcttgct gcgctttccc
     1681 agagcttact tgctcctgaa aatattgccg aagcataagc aagaagaagg ccaagagcaa
     1741 gtgatgctat cactccaaat gcagtcccct gccatactga ttttttaagc cctttgttgt
     1801 ttgtcttatc caagaatttt agtagtatgg cgattatcaa aaaagcttca agtccctctc
     1861 taaatgccat tatcaatgcc tgtaaaaatt ctgccatctt aacctcctgg gatgggtttg
     1921 ccatgcgggc aatgtttgct gtctgctacc atctttctta tactccctat ggactcttca
     1981 ctaaacgcat gttcgagcct gtgagcttct tcatgcaggg aacttttgat gccaagaaat
     2041 tccgacaaga aagcctcgat gagcctgtgc ttccttgtta tctccataga cctgcttaac
     2101 cccttcttag taagccttat cgtgctatat ggatttaggc atacaagatt tttttccgca
     2161 aggttcctta tcatctcaga tatgcttgat tttcctatat gaagatacct gcataggtct
     2221 atagacttta cagtcccttt ctcttcttgt atgtggtaga ttgcccgaag gtagttctct
     2281 gctgtctctt tgtgcataaa gttcggtaag ccgaactatt atacatatct ttccctatgc
     2341 ttttgatgaa aaaaatacag tcgatgaaaa aagcggcaac cttttgtcaa gaaccgccgg
     2401 gctgaaaagc ctcggtgcgt gttcttgagc atgctcagaa accaccctct tactccgagc
     2461 tgaagcagtg agattgcgag tggtggtttc tgacatttca aaaacttatt aaacagtctg
     2521 ataattgttt tatcaatggt cggaaaagaa ggagtaaaaa aaggcagttc aaaagagatc
     2581 catctaactg aaaggctaaa actcacttca aatgagcgtg taaaagagct cgaagatgag
     2641 atagctaaga ccaagtacaa taaacgtacc cagagagctg taggtttgat gaaagcaaag
     2701 cttgccatgc ttaaagacag ggcaagccaa cgttcaggcg gcggcaaagg cgatgaccgc
     2761 ttctctgtca gaaagacagg cgacggtact gctgttcttc ttggatttcc ttctgtaggc
     2821 aaatcaaccc ttctaaacaa ggtgacaaac gcgaaatccg atgtcgctgc ctattcattc
     2881 acaactctaa aagcggtccc tggccttatg aaatatggcc atgcaaagat ccagataata
     2941 gacgtgccag gcatagtctc aggcgcagca gcaggccgag gcagaggaaa agaggtcctg
     3001 cagatgctaa gaaatgcaga cctcatcttg atactgatag atgcgttgta tcctgagcat
     3061 tatcctgctc tcttagaaga gataaaagag acaggtattc gggtcaacga gcaaaagccc
     3121 cgtgtgaata tagtaaaaaa agacaggggg ggtatctcga taagcgagac agtgcccctg
     3181 acaaagatag attatgagac catatcaggc atagcaagag agatgaggct caacaattgc
     3241 cagataacca tccacacaga tattgattct gaccaattga tagatgttat tagcgcagac
     3301 aaggtgtata ccaaatctct ggctgtgctt acaaagattg atctcctatc agatgagcag
     3361 ctcaaggagt taaaagcacg cgtaaagcca gatgtctgca tctcagcaga ggcaggcata
     3421 gggatcgaag atctaaagaa agcgatctat gacaagatga ggttcatgag ggtatttctc
     3481 aaggaagtca ataaaaaacc ggatcttgag gagccgctga taatgtttaa gggagctacg
     3541 ctcagggatg tttgctcaaa actgcataaa gactttgttg agaagtttaa tcatgcccgg
     3601 ctctggggca aatcagctaa attcgaaggc caggccttca gaaagcttga caaggaatta
     3661 tgcgatggag atattgttga gttgcatata agatagatga tggataagcc cttattgaag
     3721 tacccttctt gggaggcata tcattcttgg caaaattaga taggaacttc aagcatttgc
     3781 tcggaaagta atttttcttt gttgttggat gacagttaga taaaaatatt taaatggtaa
     3841 tcatttaatg gaagtactat gaaagagaga tatttaccaa acaaaaaaaa attattcgct
     3901 ccctgatgcc tggtttagtg atttctttgt cgatatctct tatgtcgttt ttcttgacaa
     3961 gactaaaatg gggtgatatc gataatacta aaactgcatt gattcatagt ggaatattat
     4021 ttattatctt agtcgtgttt gtatcattgg ggatatactt gatattaagc ttgctaaatc
     4081 cataagcaaa acctttaatt accccttatt taatctttga tattatgttc gacaaacaaa
     4141 agcaggactg ccttggtaag gttgacaaaa gcaacaagca gtcgatagac aaagggataa
     4201 aaaggcttgt ggatattctt aacaagagca gggattatta caccacctcg tcctgcgcag
     4261 gaaggatagt tctcatagca aaaaagtcgt acaaaaagtc agagatgagg tttgttcatt
     4321 cgacccacag caaagcatcg ttcagcaaga taaagaaaga tttacttcaa gcctatccat
     4381 caggtagggt ggatgggaac gtgggtaaac gatcatccag ccaataccaa ggctatccgc
     4441 agggcggtag gaaaggggcg gtgagtggat atataggatc caagagcgac atctggctgc
     4501 gccaggaatc tttcatcctg catgtctgct gcaggacaca ggaagcagca gcaaaattac
     4561 tgaatatggc aagagactta ggcataaaaa gagcagggat aatctatctt ggaaaaagga
     4621 taatggtgga gataatcggc actgaatcga tggagacgat aatagcaaaa aaaggaaaat
     4681 tgctggtcac ggatgattat cttaagctgc ttctagatga agcaaacaag aaattaaaaa
     4741 gaaattgcaa aaaactggaa gaactgtatc tatctctcta atacccaata tccatacccc
     4801 ggctttaagg tttgcaatga gttatattcc tttccaggga tatagctctg ccagcttccg
     4861 ttataggcat atactggatt attgtaaaga tcagacacat ctgttccagt cagattggga
     4921 tatccgacaa gcccaaaaag agtataagat acattgttca ccctttgccc taccacggtc
     4981 agtatgcttt cctcatccat atttatccag aaacctattg tctcatttac tgagagaagg
     5041 ttggaccatt ctgcgccgct atacccccat attgcactaa acttccctgc aatgctttcc
     5101 aaaggcttat cgacatcaag aggtattgag actaggttcc atccctttct gagcgatata
     5161 ttgaatgaat actggcaggg ataggttgca ttgtcaggga tgttgcaatc agatgcaagg
     5221 ctagtatcag cacagcatga gttagaatag gtaaatatcg ttcgatagct atcctcgctg
     5281 caagttgtgt tgtagctaga ccatgccgaa gtgcagtagt tgcaacttga attcatatat
     5341 tcaaatgatg tttcattagc tatttcgttg catagattag aatcgtatag tgtcttcttc
     5401 cttgcctgag tgatacggtc cataatgagg caggttgagg tattatacca atcgctccat
     5461 gggctttcta tcatgttagg aatgcagtaa tcgcagtttt caatcgattg gtattcaacc
     5521 gtcgtagtat tttctgcctc tccgcaataa ttggcatcat aagctatagc taccctgctt
     5581 tggttcagca agtcctctgg caaacattgt aggtttaccc attgagtata ccgtgtttgt
     5641 atatcaggag tgcaataatc gcatgcctca gttgccctat attcgataat agttatgttt
     5701 gaaacttcgt cgcaaaaatg ggcgtcatag gttatctttg tccttgtctg attcatcttg
     5761 tcattgggat ggcagcttat gttttgccaa tccgaatata cctccaagag gttgggggtg
     5821 caataatcac atgcctctat cgccctgtac tcaatcgtgg tgctgtttgc aacctcgccg
     5881 caatagttgg catcataggt ttttttgctt ctgctctgat tcatcctgtc gtttgataaa
     5941 cagattatat tgccccaatc agaatattca ctaacaaggt ctgcctggca gctttggttg
     6001 attatgacag cttctggcgc atgcaccctc tcggcatatg cgaagtgaag cgaccgattt
     6061 cctccgatag ccttcttcat gttgccaatg aggtcatact cgtattggat gtccttccca
     6121 ccgacattag ccacctttag cctctcaagg tcatcatatg caaactcatc agattggttg
     6181 gcaatgaggt ctttaagggt gattaggttg cctgcaggat cataattaaa ggtataatcc
     6241 tgcatgtcgc cggtcttgat atggcttatc cttaggtcat taccatatgc aaaatcagtt
     6301 tttaggccgt ttgaatagag cctttttttg ggttgcccaa actcattata ttctatgttg
     6361 tcgaggattc catcgacaga tctgaccaat ccttgctcat taaagccgta ggataaggcc
     6421 tcaccatttg gaaggactgc ccgctttagc ctgtcataag agtcatattg aaaggtagta
     6481 ttaaatgata tgccgtcgat aatgatttcc tctttcactt ccctaagcct ttcatcgtac
     6541 caatattttt ttatcatatc cttgctcttg acagaagaaa gcgttccttt tgtgatggcg
     6601 tcatatgtat agattatttc ttggtctaca gtttttctgc tcaatagcct atttgtatta
     6661 tcgtattgaa gttctatggc atttcccttg ttatcttcct gcacgatgag atttccagcc
     6721 ttatcataag tatatgacca aattccaagg tcactatcat tagttcgtgt ctttcttccc
     6781 agagcgtcat aggtgtaggt tatcttgttt gcctggctgt ctgttaagct gatcagatta
     6841 cctaaggtat catattcgta gcttgttgta taggtgtctc catcgttgta ctctttaaca
     6901 aacacgatgt ttccataggc atcatagata aaagcctttt ttatgccccc cttgtctgtt
     6961 atttgcttgc tccagtgatc ataggaagat tcaattttcg taccatcagg tgattgctgc
     7021 aaaataaccc tgcacatgat gtcatataga taacttgtac ctactacatt tgaagcaggg
     7081 ctaaacccct ggcttgaata gtaagcattg gaaaaagact tctgccggcc cctagcatca
     7141 tacgatatat cttgtgagat aaaaagaccc ctccctgctt tttgttggac aattctgcca
     7201 aacccatcat agtactggta agtatcgatt gtatcgctat actcctctct ttggcgtgtt
     7261 atagcttctt caggggcaat accgtcaaga ttataagtga tctgcagtgt gggattctcc
     7321 attgtatcat aaggccttat ctgttttacc cttcttccaa aagtgtcata tacatggtgt
     7381 gtagtaaagc cgtttggatc agcctgagat gtaagtcttc ctagattatc ataggtatat
     7441 atagctgagt tattgctggc atctatctct ctcgtcacga gtgtatatgt tgcatcatac
     7501 tcataccttg ttgaatgccc gttggcatcg atggactcta tcagattccc ataactgtca
     7561 taaaaaaacc gcatggtagg atcctcaccc ccatccgacc aatctttcct ttggatcaga
     7621 tctccttttg catcataact atattctgtc tgcctgactg tgctattatc ataactaaga
     7681 agagaagttt gattaagttt atttagtatc caacgattag catcgctatc gtatgaattg
     7741 atgattgttt tctcatcgcc tgttttaagg acatctcctt catgatatat tttaattata
     7801 tttccatagg gatcatagct atatgttgtc ttggttattt tgggttgttt tctagttgag
     7861 atacataccc ccccattatc acaaaggcgg ctttcaccac aagctgttcc tttcggatca
     7921 taatggcagc catcgctgca gcactcactt agcgtacaat tgcagttatt aaatgttgca
     7981 tatccgattg ctttgatctt gtcttcgatt tttatcacta tccctttagc tgcggccgct
     8041 gtgtatactg tcgtgtttcc agcaaatgtg cataagtttg caggacaatt tgagggagtg
     8101 ccatcaatgc aggatgtagg atgcgaataa taactttgat agtactgttg gtctccagaa
     8161 caatcgcccg gagagttggt tgcttcatat gtatcagaag ggcctgagca accctgggct
     8221 gcaaagtcac caagaatgca atcagagcct ccgatgctat tgttctcata atttctgcag
     8281 gttgaggggt agttcttgca tctttttgac tcaatatagg tcccagtgca gctgccatcc
     8341 gtgctagacc aatagcctga aatccatcta cctggaggcc agctgcagtc agggttagag
     8401 taacttcgca aagcaaaagg atcatgggta cattgccata aggattgcgc agagatgcct
     8461 ccatgatcat agcgtccatt tggatctctt atccttgccc aattgaattt caatctatag
     8521 ctggagctat tcatctgaaa agcatcggtt tctagactat ctcccgcccc atattctccg
     8581 aaattatagg ccttgcagcc ataagtttta ttaagaacgt caactgcgca tacgtgaagg
     8641 ccatgttgtt cattcggggc gaattttata ggtccagtgc atccatcgtt catctcaagg
     8701 tcagagataa aaaaggatat actcgttaaa tttatttgat cagtacttga aacatttatg
     8761 gtcaagattg ctgcaattat taataatatt aatttcttca tcttaatccc aacttagacc
     8821 atcataagta tactctgtga tatcatcgag ggtcgttaca tagcaaccct gcttttcttt
     8881 gctttgccag ttgtacacaa cactcttgaa cggattcccc tgtaggtcaa aagtgtctgt
     8941 cttgtattca agagatgctc tcccatcatc ttgatgataa tagtgctcta tctctacatc
     9001 agcaagcttt tcagtaacct tcccaaaacc acgatattct ctttcacttg aagagtaata
     9061 agcaccagtg tagttatact ttatcagagt tacagctttc cacctaccgg ccatgccgct
     9121 atcctggctt atggattgga ctacccatcc tgcaaatcca aagtcgctta ttccatcatc
     9181 gccatcattg tcaaaattgg atattttggt gtaattgatc gttgttgttc ccccatggct
     9241 gttgtttgac actctcaaga aggggtatgt tgctttgttg attagaaccc catcctcttt
     9301 gattatatcc aaagacccat caccatcgat atctgcaagc cgtacaagag agtttatggt
     9361 agatgcaaag gcaggaagtg cccattcatt taccaggctc catccggctc cattgtttag
     9421 ccatgtctta gaagatgacc cgttgttctt tatgaggtcg aaaagcccgt ctgcattgat
     9481 atcttctatc ctcactcctg tatcattgcc tcctgacaaa aacatcgctt cagtgggcac
     9541 tgtccaaatg tcatttggaa tccatccctt gccgctgtta agccagctct ttgttgctgt
     9601 tccgtttgcc ttaaggatat ctggtagtcc gtcaccatta aggtctgcta tccttacgcc
     9661 tttgtccgtc ccattttcgt caacaaagtc ctcgggcatc tgccactccg ggctaaggct
     9721 ccatcttttg ccgtcggaag tccaagtttt tttctcaaga gcggttgatt tgacgatatc
     9781 cgaatgccca tcaccattta catcaaaaac atttacccct tgatccatag gcagctgata
     9841 gactttttgc ctctcgcatg actttactat ctcttctcct tccacaggca cagtatagac
     9901 gtacagtcct gcaccacact gctcgttagc ttggaatgta acgctgtcag tgcagtcttc
     9961 atttggggaa gggtcaagtt cccagtaagt ccatgatttt gcaccaggct tccagcagtc
    10021 atttctacag ctgctgtccc ctccgcatga aagaaaatta gggacacaat acattatctt
    10081 ttcatatcgc cactcccagc tgccgtcaaa ctcatcggtg cagccgtcat agccgcttcc
    10141 tccacagctc atctcgtaag cttgcgaccg tacagagctg tctgcacctc ctcctccgcc
    10201 aaggtgttgt aggacaaagc atactggctg aggctggata cagtccccgc cactactacc
    10261 gcaatagtag aaataatcgg tttttgcttc acaccatcct ccatatccgc tgtcggtttc
    10321 ggtatcctgc caaataagct ggggctctga gcctgcgcat tgccggttgc tgcaggttat
    10381 attgcagtaa tccccatcgc atatataccc tatatcagaa tagcctgatg ggcagccacc
    10441 gggattacag tcgatatatt ggattatgcg acctgttgtt tttatgaaat ctgcttcttg
    10501 gggaaaactg atgttgctgg tgttccactg ccacccttca ttgccgcctc tattaatctt
    10561 tacaactctt tctccagctt cagccttgat tatgtcaagc ctaagatcag agtcaaggtc
    10621 aaaaaaacgc actcccttgt caagtccatc gctgcctaca aatccgccgg caaaaaaaga
    10681 gccgattgtt ttcttttgct cccaaccctc tccgttgttt atccacagct caagatcaga
    10741 tgagttatac atcctcagaa tatctgttag gccatcaaaa tttatgtcaa caagcctaac
    10801 tccattatct ttccagccat ccttcccaaa attagcgccc tgcggcagtt gccacgccgc
    10861 gtcatcttcc catcctgccg aaggggtgtt gtatccaaag gttgtggcag gtagttttgt
    10921 cgttccgtcg gctcctgctt cctggatact tgtcaaaaag cttccggtaa tatgcccaag
    10981 gtcggaatag gaaaataggt attttcttag caggttagag tt
//
LOCUS       DASRUK010000169         2805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1491022, whole genome shotgun sequence.
ACCESSION   DASRUK010000169 DASRUK010000000
VERSION     DASRUK010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1491022"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..328)
                     /gene="tcmP"
                     /locus_tag="VFF28_07300"
     CDS             complement(86..328)
                     /gene="tcmP"
                     /locus_tag="VFF28_07300"
                     /inference="COORDINATES: protein motif:HMM:TIGR04474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HZX45459.1"
                     /translation="MAKGTGTLWPITPHTQAKHIILQKYLGAWIPIISKYNGKVIYVD
                     GFSGPGEYRDGELGSPLIAIKFLSRNPHHLWLGMNA"
     gene            555..2768
                     /locus_tag="VFF28_07305"
     CDS             555..2768
                     /locus_tag="VFF28_07305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrophobe/amphiphile efflux-3 (HAE3) family
                     transporter"
                     /protein_id="HZX45460.1"
                     /translation="MNILERALAKVGKLELKYFLVFLIGISIFTAFMAVGMTKVTFES
                     DFAKFNPKGIPIIELNDKIDKEFAHLSTFLITLEVDEGIDDIRDPDVMNFLINLDKHL
                     RDEQKIQDVQSAALFFQQGVPESLEGVKQTLSQIPQSDIFFNKAKSVTPVFLEADVGD
                     DSAKIKEITLRVREIIANAGKPEGIKATVTGEPALGSVIFDLIIRDGIVTLFITLIAI
                     FVLLLIITRSFQNAIVIITPVALGVIWTIGAMGWLQVPITVATAAIGAMILGLGTEYS
                     IFLNSRYKEERIRRDWQDAIIEALSTTGVSTMSSGITTMIGFFALMFSFFPMLSDLGF
                     SLGIGIGFVLASTMLGGPLVMIIQNKLKQQKGQKEAEYAIFDKYGKLVAHAPWLVLIV
                     MLVVTGFVYIGSQRIVQEEVDFFNVLPQDLEELKAFRFMQNEFGDTTGVRYYVSLDAA
                     NSGSDEPEDIRDPRVVRYINTISQKAREIEYAISVSSISTEPGIIPATLREQKELLQS
                     GLINNDFSATTINIEFDSDAAKNNKEEIIREIYEIAESTDKPAGVLIQPAGGMIEEHE
                     LNKILNPDSSRTGIISFVAIIIFLFIITRSIKYTILPLITVILAILWVFGLIGYFDIP
                     FNSIISSVVSMTIGVGIDFGIQLALRFRHERQVNQLDKKTAMANTLKHTLYPMVVTVI
                     AALVGFQAMRFGKLTLMGNLGTAMSFAVLSSMIVAVTIVASLILIFERALRTNYP"
BASE COUNT          830 a          585 c          605 g          785 t
ORIGIN      
        1 ggtaaagtgt cattgggcct cacctctttt ttgaggctca tcattttacc aacagataat
       61 cacagaatta gccatcctat aagtgttacg cattcatccc caaccacaag tggtggggat
      121 ttctgctaag aaatttaatg gcaatgaggg gtgaacccag ttcgccatct ctatattccc
      181 ctggacctga gaaaccgtct acataaataa cctttccatt atatttgctt attatcggaa
      241 tccaggctcc aaggtatttt tgtagaatta tatgttttgc ctgagtatga ggagttattg
      301 gccataaagt gccagtgcct tttgccataa acacgacttc gtaaacttta tttataaacc
      361 tttcgcgcaa ctcttttggt atgtccagtg agtaaatttg caggttattc tctaaaataa
      421 caagattgca cttgacataa taccttacgg taagattcta tgctttgctt gttattcttt
      481 atacaacatg cccgcgacta ccaaaccctt ataaaccatc caataatccc aaccctttct
      541 acgttggggc taacatgaac atccttgaaa gggcactcgc aaaggtaggc aagttggagc
      601 tgaagtactt tcttgtcttt ctcataggca tctctatatt tactgcgttc atggcagtcg
      661 ggatgacaaa agtcaccttt gagtcagact ttgccaagtt taacccaaaa ggaataccca
      721 taatagaact aaacgacaag atagacaaag agtttgcgca tctcagcacg tttcttataa
      781 ccctagaggt tgacgagggg atagatgaca taagagaccc tgatgtgatg aactttctga
      841 taaatctcga caagcacctg agggacgagc aaaagataca ggacgtgcag tctgctgccc
      901 tatttttcca gcaaggcgtt ccagaaagcc ttgagggagt caagcaaacc ctaagccaga
      961 tacctcagag cgatatattc ttcaacaaag caaagtctgt aactcctgtc tttcttgaag
     1021 cagatgttgg agatgacagc gcgaaaataa aggagatcac tttaagggta agggaaatta
     1081 tcgcaaatgc tggaaagcct gaaggaataa aggcaactgt aacaggcgag cctgccctag
     1141 gcagcgtcat ctttgacctg ataataaggg atgggatagt gaccctgttc atcacgctga
     1201 tagctatatt tgtcttgctt ttgataataa cccgttcttt tcaaaatgca atagttatca
     1261 taacaccagt cgctcttgga gttatctgga caataggcgc tatgggatgg cttcaagtgc
     1321 caataactgt tgccacagct gcgataggcg ctatgatact tgggcttggg acagaatatt
     1381 ccatatttct aaactccaga tataaagaag aaaggataag acgagattgg caggatgcca
     1441 ttatagaagc tctttcaacc accggagtca gcaccatgag ctctggcata actacgatga
     1501 taggcttctt tgcgctgatg ttttcctttt ttcccatgct aagcgatctt ggattttccc
     1561 tgggtatagg cataggcttt gtccttgctt caacgatgct tggcggaccg ttggtcatga
     1621 taatccagaa caagctaaag cagcagaaag gacagaaaga agcagaatat gcaatctttg
     1681 acaaatatgg aaaactagtt gcccatgcac cctggctagt attgatagtc atgctcgtag
     1741 tgacaggttt tgtctatatc ggctcacaaa ggatagtgca agaagaggtt gattttttca
     1801 atgtgctgcc tcaagactta gaagaattaa aggcattcag gttcatgcag aacgagtttg
     1861 gtgatacaac cggcgtgagg tattatgttt cccttgatgc tgcaaattca ggctctgatg
     1921 agccagagga catacgggat ccaagggttg tgaggtatat caatactatc tctcagaagg
     1981 caagagagat tgagtatgca ataagtgtaa gcagcatctc cacggagcca ggcatcatcc
     2041 ctgcaacact aagagagcaa aaggaattac tgcaaagcgg gcttatcaac aatgactttt
     2101 cagcaacaac catcaatata gagtttgatt cagatgctgc aaagaacaac aaggaagaga
     2161 taatcaggga gatatatgag attgcagaaa gcacagataa acctgcagga gtccttatcc
     2221 agcctgctgg agggatgata gaagagcatg agctaaacaa gatacttaac cctgactcgt
     2281 caaggactgg catcatctct tttgtcgcta tcatcatatt cttgtttatc attacacgat
     2341 cgataaaata tactatcctt ccgttgataa cagtaatcct tgctatcctc tgggtttttg
     2401 gcctcatagg ctattttgat attccattca acagcataat atcatctgtc gtgtcaatga
     2461 caataggtgt agggatagat tttggtatcc agcttgcact gagatttcgg catgagcgtc
     2521 aggtaaacca acttgacaaa aaaacagcta tggcaaatac cctaaagcat acattatatc
     2581 ctatggtggt cactgtgatc gctgctcttg tggggtttca agccatgagg tttggaaagc
     2641 tcactcttat gggcaatctt ggcacagcga tgagctttgc tgtcctttca tcgatgatag
     2701 tcgcggtaac tatcgtcgcc agtcttatac tcatctttga aagggctttg cgcacaaatt
     2761 atccctgaat cctgttaaaa tatgatagtc ttgccctgtt atgca
//
LOCUS       DASRUK010000170         3370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_662255, whole genome shotgun sequence.
ACCESSION   DASRUK010000170 DASRUK010000000
VERSION     DASRUK010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662255"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..287)
                     /locus_tag="VFF28_07310"
     CDS             complement(33..287)
                     /locus_tag="VFF28_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009544611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HZX45461.1"
                     /translation="MNSIMALGLMAGALTTFSFLPQVIKTLRLKETKDLSLWMYVLFC
                     TGIFTWLIYGIIIKDIPVMIANAVSLILASIILFAKLRYR"
     gene            complement(523..1284)
                     /locus_tag="VFF28_07315"
     CDS             complement(523..1284)
                     /locus_tag="VFF28_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45462.1"
                     /translation="MADSLENIVENRPKSDNLETKVNGQGHNSPHIIKFLEEKAKQRK
                     PRSTLGKILDYTIAAGCIVGSYLAVGNIALVANVISLIGERIVNWRRGRDTPSRQTRN
                     SALMASLMSIPGYFAFKWMNSLYDVTTWSGLLKRYAVQNFAYIPANTLIGNAIGYPLH
                     FGTTKGMWDYGIKDLGWRNYKTGLKYFSWPNLAAARFLPDYTHYPVALGAGMLWRTTA
                     GSRYLHEADPYKYEHRIIDGKPANGAENKSYKKAA"
     gene            complement(1422..2066)
                     /locus_tag="VFF28_07320"
     CDS             complement(1422..2066)
                     /locus_tag="VFF28_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008083791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HZX45463.1"
                     /translation="MSIEENIGLIKKELPRHVKLVAAAKTRSIEEIKQAIEAGVDAIG
                     ENYIQEAEAKYKEFKGKIPIHCIGHLQSNKAKKAVEIFDMIETIDSLNLAKEIDKQSQ
                     KLNKIMPVLVEINSAREESKAGCMPDHAQQLVKDISLLKNIKVLGLMTMGPIKGDIAA
                     AFRETKSLFDHIKSLSIPNTDMQILSMGMSGSYKIAVKEGANMVRIGTKIFGPR"
     gene            complement(2056..3042)
                     /locus_tag="VFF28_07325"
     CDS             complement(2056..3042)
                     /locus_tag="VFF28_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HZX45464.1"
                     /translation="MTHDVVSIGNPLMDVLIEIEEEFLKELDLVKGNMHLIDEKGIRQ
                     IEKKLKNFEKKRSPGGSEANTLALLSMLGHKVVYFGKVGKDDYGKHYHEQLQKSGVLS
                     KVIQVDGMTGRAITLITPDCERTFATSLGVCNLLKDSEISEIDIVGAKYLHLTGYILD
                     SELTRKAAIRALNIAHSNNVKVCLDLADINVIKRHKDLIISLAKDYVNIIIANDKEAK
                     EFTGLDPEKAINVFSALAEIAIIKLGEKGSIIQSKGELIRVPGFKAKVVDTTGAGDAY
                     TAGFLYGLLNDLSLKVSGLIASFIASKVVEVKGARIDRIPIKDIEKLKLNVD"
     gene            complement(3078..3323)
                     /locus_tag="VFF28_07330"
     CDS             complement(3078..3323)
                     /locus_tag="VFF28_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45465.1"
                     /translation="MADKKPESGKEEQIGFHKGALSTLAKEREEMYRILQIVEQLMQM
                     HIKGLKDLGVDLEAAAKEAQKQSAAKKAKKPIEDMIR"
BASE COUNT          813 a          735 c          653 g         1169 t
ORIGIN      
        1 atgttttact tcttatatcc cagttccttt atttatctat atcttaattt tgcgaaaaga
       61 attatagacg ccaatatcag tgatacggcg tttgcaatca ttacagggat atctttgatg
      121 atgatgccgt agataagcca tgtgaagata cctgtgcaga acagcacata catccaaagg
      181 gagagatctt ttgtctcctt taaccttaaa gtctttatga cctgcgggag gaaggagaaa
      241 gtggttagcg ctcctgccat caagcccaat gccattatcg agttcataac tgccttttta
      301 gaggcattgt ttaaattatt tttggaaatg acagaatcgc ttttggcaaa accactttaa
      361 actgctattt agcgacaaca atccagcgat ttctgatcat gctcaaaaac tttcgctcca
      421 ctctctggag ttctattgcg gagtttttga caaaggtgag agggcaaggc tggcgaaagc
      481 ccgccctctc taaaatcaag gctgattctc cttgaatgtg atttatgctg ctttcttgta
      541 gcttttgttt tctgctccat ttgcaggctt tccgtctata atcctatgct catacttgta
      601 agggtctgcc tcatgcagat accttgagcc tgctgttgtt ctccataaca ttcctgctcc
      661 taaagctaca ggatagtgag tgtaatctgg aaggaagcgt gctgcggcaa gatttggcca
      721 tgaaaaatat tttaggcctg tcttatagtt tctccagcca aggtctttta tcccataatc
      781 ccacatacct tttgtcgtgc caaagtgaag gggatagcct attgcgtttc caataagggt
      841 atttgctgga atataggcga agttctggac tgcatacctt tttagtaaac cagaccatgt
      901 tgtaacatca tagaggctgt tcatccattt gaatgcaaag tagccaggga tggacatcaa
      961 ggaggccatc aatgcagaat ttctcgtctg tctggatggg gtatctcttc cccttctcca
     1021 attgactatc cgctcgccta taaggcttat tacgtttgca accaaggcaa tatttcccac
     1081 tgctaaatat gagcctacaa tacagcctgc tgctatagta tagtcgagaa tcttgcctag
     1141 agtagacctt ggctttctct gctttgcctt ttcttcaaga aatttgatga tatgcgggct
     1201 gttgtggcct tggccattta ctttagtctc aagattatca gatttaggtc tattctctac
     1261 tatgttctca agtgaatcag ccacattaca ttcgccgata gaaacggtct ttagagcctt
     1321 tataaaggtt atgtattgta actacttatt agtagtttct ttatggcaaa ataaaatgta
     1381 gattgataag tatcgacaat cgctttttgg tagaaagatc actatcttgg gccgaatatc
     1441 tttgtgccaa tcctgaccat atttgcgcct tcttttacgg caatcttgta gctgccgctc
     1501 atacccatgg ataggatttg catgtcagtg tttggtatgg ataatgactt gatatgatcg
     1561 aacaatgatt ttgtctctct aaaagctgct gcaatatctc ctttgattgg acccatagtc
     1621 ataaggccca ggacttttat attttttaga agggatatat ctttgacgag ctgctgtgca
     1681 tgatctggca tgcatcctgc cttactttct tcccttgcgc tgtttatctc aaccagtaca
     1741 ggcataatct tattcagttt ttggctttgc ttgtctatct cttttgcaag atttagagag
     1801 tctatcgtct ctatcatgtc aaaaatttct actgcttttt ttgccttgtt tgactgcaag
     1861 tgaccgatac aatggatagg gatcttgcct ttgaactcct tgtattttgc ctctgcttcc
     1921 tgaatataat tttctccgat agcatcgact cctgcttcta tcgcctgctt tatctcctct
     1981 atagaacgag tctttgccgc tgccaccaac tttacatgcc tgggtagctc tttttttatt
     2041 aagccaatgt tctcttcaat cgacatttaa tttcaacttc tctatgtcct ttattggaat
     2101 cctatcaatc cttgccccct ttacttctac aactttggat gcgatgaatg atgcgataag
     2161 gcctgagact ttcagactta ggtcattgag caggccatat aggaagcctg cagtatatgc
     2221 gtctcctgcg cctgttgtgt cgacgacttt tgccttgaag cctggaactc ttatcaactc
     2281 acctttgctt tggataatgg agcccttttc accgagcttg attatagcta tctctgcaag
     2341 agctgaaaaa acatttatag ctttctccgg gtcaagacct gtaaactctt ttgcctcttt
     2401 atcgtttgcg attattatgt taacatagtc ttttgcgagt gagatgataa gatctttatg
     2461 gcgcttgatc acgtttatat ctgccaggtc aaggcaaact tttacgttat tgctatgggc
     2521 gatatttagc gcccttatgg ctgcttttct tgtcagctcg ctatcaagga tatagccggt
     2581 aagatggagg tattttgctc cgacgatatc tatctcgctt atctcactgt ctttgagcag
     2641 attgcatacg ccaagggatg ttgcaaatgt tctttcacag tcaggagtaa tcagtgttat
     2701 cgcccttccg gtcatgccgt ctacttggat gacttttgag aggacaccgc tcttttggag
     2761 ctgctcgtga taatgtttgc cgtaatcgtc ttttcctacc ttgccaaagt atacgacttt
     2821 atgaccgagc atagaaagaa gggctaaggt gtttgcttct gagccgcctg gcgagcgctt
     2881 tttctcaaag ttctttagct ttttttctat ctgtcttatg cccttttcat ctataagatg
     2941 catatttcct tttaccaggt caagctcttt tagaaattct tcttctatct ctatcaggac
     3001 atccatcagg ggatttccaa tactaaccac atcatgagtc ataccaataa agaaaaagat
     3061 actgaataaa aatcttacta tctaatcatg tcttctatgg gctttttggc tttttttgct
     3121 gctgactgct tttgtgcttc ctttgcagct gcttccaggt caacgcccag gtcttttagg
     3181 cctttgatgt gcatctgcat gagctgctcg acaatctgca aaatcctgta catctcttcc
     3241 ctttcctttg ctaatgtcga taaagctcct ttgtgaaaac caatctgctc ttcttttcct
     3301 gactctggtt ttttgtctgc cataagtgaa taaaattatg ttcttgttta aaaagttacc
     3361 tataaaaggg
//
LOCUS       DASRUK010000171        21149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2321736, whole genome shotgun sequence.
ACCESSION   DASRUK010000171 DASRUK010000000
VERSION     DASRUK010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 21149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21149
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2321736"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VFF28_07335"
     CDS             complement(<1..521)
                     /locus_tag="VFF28_07335"
                     /inference="COORDINATES: protein motif:HMM:NF013348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HZX45466.1"
                     /translation="MVYISQSGQKLSNKAFLDYFEKKVRKTIRVWGLLGKKEKILVAC
                     SGGKDSTTALYLIKKIVAGNRNISVEAIHVDPSIGEYSKINRENIVRFCSENGIKLHL
                     TSFREEFGYSLCYIKGLVKEYKSCRVCGILRRYILNKKAKEASATKIVTGHNLDDEAQ
                     NVIMNIFQNHVEI"
     gene            600..1355
                     /locus_tag="VFF28_07340"
     CDS             600..1355
                     /locus_tag="VFF28_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HZX45467.1"
                     /translation="MAYIWLYTLLSVFIVSIISLIGILTLAFRESGLKRILLFLVSFA
                     AGALLGDALIHILPEVIEEYGFGLKVSLSILLGIVLFFILEKFIHWRHCHAPVGKSHP
                     HPLAFMNIIGDGIHNFIDGMLIAGSYLASFPLGITTTIAVILHEIPQEFGDFGVLLHA
                     GMTKAKALFFNLLSATAAILGAVLTLIIGSSFTNLTEFILPITAGGFIYIAGTDLIPE
                     LHKKCEPASENVYQLIAFILGIGAMVLLLLFEF"
     gene            complement(1352..2227)
                     /locus_tag="VFF28_07345"
     CDS             complement(1352..2227)
                     /locus_tag="VFF28_07345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZX45468.1"
                     /translation="MHDHRESKKNLIFGIVLNGSFTLVELAGGLLSNSLALISDAIHD
                     LSDTIAIGISLYANEKAQAAPTKTKTFGYGRTTVLAALINAIALILITGFIFYRAYLR
                     ILEPKPVEGMIVFFIALVGMIVNGAIALRMWKSKDKDINMRSIFWHITEDFLGWIGVL
                     ISGVVIIFTDFYIIDPIMSILIGLIVLYSAWDIVKEGVNIFLEGVPRGIDVKKVEDSI
                     KKIDKVKDVHDTHIWSLGPGNYSISAHVAVPNMKVDETYPILCRIHSELKKFHIEHTT
                     IEFECSSCDPRRVCH"
     gene            complement(2230..3465)
                     /locus_tag="VFF28_07350"
     CDS             complement(2230..3465)
                     /locus_tag="VFF28_07350"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HZX45469.1"
                     /translation="MIYAVKVPKTWWFSHKKTKEKLPAGWYHADQLAKKDLIAYPISK
                     EVRDKDHLLFNNEKKKWDFRSKAIPSKIPLNDDFLRLAGYYLAEGYLKEKVTNTYLSF
                     TFNIKEQELGNNVAYIVKKLFDIEAKTKTIPKKKTLIVEVNNVWITRLFKTLFGKGAQ
                     SKRIPHELMLLPAEKQKSLIYGLWKGDGYFNIKRPRAGYTTISRELCQQMKTLLLRQK
                     ITPSIYEEEKKIVKGVNHRKSYRIHIGDRTSLECLAKILCIDFKCSKDTATHSWFDNE
                     YIYTPITEIAKIPYRGDVCNLEVDDCHSYLTESLAVHNCGDLMWVYIKVGKNKEGKEY
                     IEDIGVKTFGCVAAIATSSMITDLARGKTLEEAKKIGRDDIKDALGGLPPIKVHCSNL
                     SADGLYKAIEDFESKKGKK"
     gene            complement(3655..3753)
                     /locus_tag="VFF28_07355"
                     /pseudo
     CDS             complement(3655..3753)
                     /locus_tag="VFF28_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012965625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
     gene            complement(3759..4907)
                     /locus_tag="VFF28_07360"
     CDS             complement(3759..4907)
                     /locus_tag="VFF28_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HZX45470.1"
                     /translation="MKAYLDNGATTKVDERVVEAMMPYFTETYGNASSLHTPGQEAKS
                     GLEQSREIIAKRINAKSDEIIFTSGGTESDNLAVKGYVYANKDKGNHIITTKIEHHAV
                     QYTCKSLEKEGFKVTWLDVDKEGFVDLKKLSSAITDMTLLVTVIHGNNEIGTLQDISA
                     IGKICDENKVAFHIDAVQSFTKVPIDVVSQKVTMASFSAHKIHGPKGIGALYVKKGTI
                     LKKLNDGGGQESNLRGGTENVAGAVGFAKAVQLSTEQECKKMEKLRDNFINQVEKEIP
                     EVKLNGPRRMRLCNNANIIFKFIEGEGMLIKLDDRGIAVSTGSACSSRDLTPSHVLTA
                     IGVPHALAHGSIRFTLSRFTTKEELDYTVDNLKVMVKELREISPLWKR"
     gene            complement(4891..5199)
                     /locus_tag="VFF28_07365"
     CDS             complement(4891..5199)
                     /locus_tag="VFF28_07365"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZX45471.1"
                     /translation="MKCSSYESFFETISGKTRMKIITCLATGAKSVSEICASVKEEQS
                     KVSHNLRKLLKCHFVDFKRSGKLKVYELNKDTILPILNLAEKHARKYCREECLHESLS
                     "
     gene            complement(5273..6202)
                     /locus_tag="VFF28_07370"
     CDS             complement(5273..6202)
                     /locus_tag="VFF28_07370"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZX45472.1"
                     /translation="MYDVIILGAGVSGLSAAVYCGRFNLKTIVIGESGGAIALSSEVT
                     NFPGYESIPGIELADRIKKHALAYGAELIEGKADKIEKDGKFLKVFFGKKSYEAKAAI
                     VATGTDWRKLNVPGEKQYTNKGVHYCALCDGALYKNKVISVVGGSDSAAKEALMLSQF
                     GKKVYIIYRGDKIRADPKNCSLIEANKKVEIIIKTNVVGIKGEKFVKSVVLDRPYKGS
                     TYLMLDAIFVDIGREPVSHIVRDIGVNVNEKNEIMVDSDGKTNVKGVFACGDVINSRF
                     KQAVTAVAQGVVAAYSAYQYISDEVPMIGSNSD"
     gene            complement(6208..6444)
                     /locus_tag="VFF28_07375"
     CDS             complement(6208..6444)
                     /locus_tag="VFF28_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005397046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HZX45473.1"
                     /translation="MPTVTVYSTPTCPWCFKAKDFLKDNNIKFVEKDVSLDDDARDEM
                     AEKTGQMGVPVIVVQGHDPIIGFDQQALKEALNL"
     gene            complement(6481..7116)
                     /locus_tag="VFF28_07380"
     CDS             complement(6481..7116)
                     /locus_tag="VFF28_07380"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF013030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HZX45474.1"
                     /translation="MRSEIVPSIIASNQKELDERVDKVKDFFSTIQLDVMDGKFVPTR
                     SIDFNFKVPECRCQVEAHLMVEDASSWVSKHGKKADTILAHIESCKDPKGMVNQVRAM
                     GKRIGFALNPKTPIERIKEVLDDVDEVLIMTVNPGYYGSPFLPETLEKVRKLREIMPT
                     SDIEVDGGISPLTLLTAKQAGANYFVCGSYIQKSGDISKAAKELNLKIGKI"
     gene            complement(7189..7344)
                     /locus_tag="VFF28_07385"
     CDS             complement(7189..7344)
                     /locus_tag="VFF28_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45475.1"
                     /translation="MTFCKTCGMKITNIGHLCNPQCNRRIYICDICGIITTEKDLLCK
                     PKLFKLK"
     gene            7432..8070
                     /locus_tag="VFF28_07390"
     CDS             7432..8070
                     /locus_tag="VFF28_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45476.1"
                     /translation="MAFTQIAAAGEYMDSLFVGMRHFPTKKVILLSTPENLGIAEKAR
                     KDLLKFKIPVFIKKLEGDLWEAVFQAVKEIKEVEGESDIIINSASGDSGLSRCAICSA
                     AFVNGLKAFTVVKDEVILMPILKFSYYKILTDKKMEILKYLFSNQDCCSSLDELGRKL
                     KMSLPLVSYHINGNLKSEGLKEMGLIVAEEFKGKVRITLTLLGRLIVKGYVR"
     gene            8124..8951
                     /locus_tag="VFF28_07395"
     CDS             8124..8951
                     /locus_tag="VFF28_07395"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HZX45477.1"
                     /translation="MAIWNAVLRISIAVVALIFLFYLLTFLEKRFTSKVKSLNQFHNL
                     KIFFRLVNYFILLVTLVIFLFAYVGSWAGLGLGLGFFTAALGWALQKPITGIAAWLIV
                     IAYRPFKIGDRVSIGGVRGDVADIRLTHIYLNEVGGIAAGEENSNRVIMIPNSILFDQ
                     NIINYTKSSPYVLDEVGFSVTFSSDIDIAKKIVLDSTRRITQEVIERIKQEPYIRIAL
                     TTDGVKLNVRYMSPAVRLYEMSTKVAEEIVRGINSESKVQFAYPHTELVFGNTKSKR"
     gene            9104..9280
                     /locus_tag="VFF28_07400"
     CDS             9104..9280
                     /locus_tag="VFF28_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Em GEA1 (EM1)"
                     /protein_id="HZX45478.1"
                     /translation="MAKGEMSVKEAGRKGGEETSRTHGRQFYQDIGHKGGQIGGEKGG
                     RRVKELIGKAKRGE"
     gene            9282..9446
                     /locus_tag="VFF28_07405"
     CDS             9282..9446
                     /locus_tag="VFF28_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HZX45479.1"
                     /translation="MVRGEISVREAGRRGGEETSRTHGKEFYQEIGHKGGQRGGPRVR
                     ELIERGKESE"
     gene            9457..9804
                     /locus_tag="VFF28_07410"
     CDS             9457..9804
                     /locus_tag="VFF28_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012898754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HZX45480.1"
                     /translation="MAEEKKGEMSVKDAGRKGGEKTSQAHGKEFYENIGHKGGQKTSQ
                     AHGKDFYEDIGHKGGERTSESHGKDFYEDIGHKGGERTSRSHGKEFYQEIGHKGGQRV
                     RELIKEGKKTEEG"
     gene            9811..10041
                     /locus_tag="VFF28_07415"
     CDS             9811..10041
                     /locus_tag="VFF28_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HZX45481.1"
                     /translation="MAESKGDISVRDAGKKGGQETSRTHGKEFYQEIGKKGGQETSKT
                     HGREFYQDIGRKGGERGGPRVRELIRRAKGED"
     gene            10057..10245
                     /locus_tag="VFF28_07420"
     CDS             10057..10245
                     /locus_tag="VFF28_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012899844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HZX45482.1"
                     /translation="MVRGMRARAESKGEISVREAGRRGGQETSRTHGKEFYQEIGHKG
                     GQRVRELIQQGKRAEGEE"
     gene            10245..10379
                     /locus_tag="VFF28_07425"
     CDS             10245..10379
                     /locus_tag="VFF28_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Em GEA1 (EM1)"
                     /protein_id="HZX45483.1"
                     /translation="MEGRSEEKERSARDRGGMTVRQAGHKGGQRVRELIKEGKETEER
                     "
     gene            10385..10615
                     /locus_tag="VFF28_07430"
     CDS             10385..10615
                     /locus_tag="VFF28_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HZX45484.1"
                     /translation="MAEESKGDISVKEAGKKGGEETSRTHGKEFYEDIGKKGGEETSR
                     THGREFYQEIGHKGGQRVRELIKEGKQAEGQE"
     gene            complement(10644..12008)
                     /gene="ligD"
                     /locus_tag="VFF28_07435"
     CDS             complement(10644..12008)
                     /gene="ligD"
                     /locus_tag="VFF28_07435"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HZX45485.1"
                     /translation="MALLEYAKKRDFARTPEPRSIIKKKDVNSFVIQRHNARRLHYDL
                     RLEYQGVLKSWAIPKGLPYYTEEVRLAVQTEDHPVEYLNFEGRIPAGNYGAGTVDIWD
                     SGRYTNLSIKDKEAVPLPQAMEKGHIHFFLKGNRVEAELHLIRTKGKNWIVKRAKSYM
                     KEYENKGISNADGRQVSLTNQNKKIYSDIKKGDYIQYYEDASNLILRHITDRPLSLYR
                     FPNGVEGAKFFQKNMGGSVPDWIKTVMIESERRKTEYMIITHKASLIYFANMVAEPHI
                     TTSKHDKPLVPDKIVFDLDPGDVPVEKLKETALKLRKILSDIGLKPYIMTTGGKGYHI
                     SVPIKREYTSDKVKDFALKVADSLVLSDPNFYTTSLLKTKRKNKIFIDVNRNSAMQTS
                     VCPYGARAKPRLTIAAPFEWDDLTIVFPDSFNIENYPKKDAWRDFFDNARSLKNVINN
                     LTDY"
     gene            complement(12018..12440)
                     /locus_tag="VFF28_07440"
     CDS             complement(12018..12440)
                     /locus_tag="VFF28_07440"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HZX45486.1"
                     /translation="MKTIYDKLKMEHDMVKDMMKETMKTKDKAKFMDIQENLIPHLDG
                     EEKFLYPLLMEKEHSKQLAYEALEEHEVVRELLSKIEREKEEIRWWAKFKVLMENLEH
                     HIIEEERELFAHAKKDLVKGEEEQILADYDQFKGEAIA"
     gene            complement(12482..13972)
                     /locus_tag="VFF28_07445"
     CDS             complement(12482..13972)
                     /locus_tag="VFF28_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZX45487.1"
                     /translation="MDKLSALKSMKGREAVTSGACDTLWLSANRGNKQTLDKDIKTDT
                     LVIGAGIAGLSTAYNLLEKGKKVVVLEDGLLGSGETGRTTAHLSYTMDDRLFEIEKIF
                     GERGAMIAAQSHAAAVETMYNISKKEKIDCDYTGVDGYLFSEHGKILEKELKAYKKAG
                     FKPKLSDTAPDNTFDMGANLRFPKQAHIHPMKYLSGLASTIKRKGGEIFTGTHAQEVG
                     AGKVVTADGKRVHAKNIVIATNVPIMDKKIVLKQAAYRTFVIAARIKKGLLSDALYWD
                     TLENYHYVRLQKYDRRYDHIIIGGEDHKTGQANDGEDRFLRLEKWARERFPITSIDYR
                     WSGQVIEPLDYMGFIGRYPGKKNIFISTADSGQGMTHGTIAGILISDLIIGRKNIWEK
                     LYDPSRKKAVGRFLAHNMNVAGQFVKYLKSDREVRPGEGGRIGKKVMINDGKVRKFSG
                     ICPHMGCGVLWNSTEKTFDCPCHGSRYTAYGKVVNAPANHGLEVED"
     gene            complement(14076..14234)
                     /locus_tag="VFF28_07450"
                     /pseudo
     CDS             complement(14076..14234)
                     /locus_tag="VFF28_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427677.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(14344..17022)
                     /locus_tag="VFF28_07455"
     CDS             complement(14344..17022)
                     /locus_tag="VFF28_07455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016949993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZX45488.1"
                     /translation="MALHKLEKLFNPTTIAVIGATNKAGSVGHALMKNLIASGFEGIV
                     YPINPKRRSILGVKTYPSISKTPDKIDLAIIATPADTVPAIVEECGASGVHGIVIISA
                     GFKEIGKKGEAMCDEIAMTARKYDMRIIGPNCLGFIRPKLKLNASFANKMALPGKIAF
                     ISQSGALGTAVLDWSVSQNVGFSYFVSIGSMIDVGFHDLIDYFGQDPETSSILIYMES
                     LTYAKKFLSAARAFARTKPIIIIKVGKSSEGAKAAMSHTGSITGNDAVFDAAFKRAGA
                     IRVNTIGQLFNCAQTLAMQPRPMGKRLAIVTNAGGPGVIATDSIISKGGTLASISPES
                     MKSLNACLPEAWSKSNPVDVLGDADPERYKKAAEVCLRDPNVDGLLVILTPQAMTDAT
                     TIAKQIVDLSKTSNKTILTAWMGEDDVTEGRKILKNGNIPVYSVPEDAVECFMEMFNY
                     SKNLELLYETPSTIPHAFKPKTQENEKIIAKVVSEGRSVMTESEAKQMLSNYDIPIAK
                     KGIAKTEAEAVETASDIGFPVVMKILSPDILHKTDVGGVKVNIQSKEHVKEAFKEIMT
                     SAKKHAPKADIHGIFIEKMTSRKYELLIGCKKDPIFGPVIVFGMGGVAVEVFKDTNIG
                     LPPLNMALALRLIEETKIYTLLKGYRGMQGADIASIQFLLYKFAYLVMDFPEIKEIDI
                     NPFAVDEKGGIVLDAKIILDPEIAGKNPRPYSHLVISPYPKEYISKYKMSNGKEAILR
                     PIRPEDEPMEAEMFTNFSEQTQRFRFFQLIKEINHEILVRYTQIDYDREIAIVAEVEE
                     EGKKKMAGVVRLIADPYNETAEFAIVVADPWQKQGIGNRFIDYVLEIAHERCIKKIYA
                     DFLKENSVMKHMFERRGFTISKIDNDAMHAELVLKE"
     gene            complement(17032..17988)
                     /locus_tag="VFF28_07460"
     CDS             complement(17032..17988)
                     /locus_tag="VFF28_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HZX45489.1"
                     /translation="MDIIYNSIFLEHDTGMHPENRKRLESLGKLKETKIESGEPYLGL
                     IHTKEYIDLVKDTCKRGGHLDPDTIVSERSYEAAVLAVGATIMASKTNDFALVRPPGH
                     HAYPDHSSGFCIFNNIAIAAKKLVNEGKKVFILDFDGHLGDGTVKIFYNTDKVLYWSL
                     HQGPAFPGGGNVDEIGEGTGKGYTINMPLPPGTGDDLFIDALERLMIIPAQFKPDVVA
                     VSAGFDGHSADLLLDLRLSCRSYYKIGHILSQRFKNVFATLEGGYNLSVLQKCIFNFI
                     DGANGNKMRFDEQATDSRIIARDEYEGRILSLEKNLSPYWKL"
     gene            complement(18036..18182)
                     /locus_tag="VFF28_07465"
     CDS             complement(18036..18182)
                     /locus_tag="VFF28_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45490.1"
                     /translation="MIPDEHCTVPANCICPKCGTKVVHVKGTPCEKMTCPKCRSNMAM
                     DKCI"
     gene            18471..19151
                     /locus_tag="VFF28_07470"
     CDS             18471..19151
                     /locus_tag="VFF28_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45491.1"
                     /translation="MVYVVSPEWFYGIDYLFDSVSVIVALLITFFSYKSYKYTSQKKY
                     LYFALSLFLVAAAFLAKILATIPIYSRQLKVETIGLVTVTSQVISKVTWINQFGVSIG
                     RFLMLIAFLILIIITLKLADKKIIGLLIYFLVISTTLVSASYEVFHLTLLIMVTLLFM
                     NYRHNFLKIKSKNSKLVMLTFFCLMISQFFFFGAGFISNLYVIGESIQLVGYLILLGA
                     FVKIVTKK"
     gene            19148..19420
                     /locus_tag="VFF28_07475"
     CDS             19148..19420
                     /locus_tag="VFF28_07475"
                     /inference="COORDINATES: protein motif:HMM:NF026297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZX45492.1"
                     /translation="MSIRRTKIEIIHDILNSIQKKGGRIKPTHLLYKSNLSYKKMLEY
                     LEELKGKGMVQEEEEEGKRMYLITDKGAEYIQEYKKIKEFSDSFGL"
     gene            19462..20091
                     /locus_tag="VFF28_07480"
     CDS             19462..20091
                     /locus_tag="VFF28_07480"
                     /inference="COORDINATES: protein motif:HMM:NF016831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZX45493.1"
                     /translation="MWRTKVRLKHSCLFGDNCKEAKVTCLNMSFNTFKKGDSYFVFHM
                     GTVFGDNYTKFLALLKKDRRVDYIESDGRTFVVLEKRPEKEIPGMVINNEVIYVKPVK
                     VDEDGYETWELAAIEKEKLMDFINHFKDSEILNIEQTKLKDIYFPRLSPNFSQHQRDA
                     LELAIEEGYYQFPKKTDLNKLSKIARLSKSTFREHLKRAEMKVLGELGR"
     gene            complement(20097..20666)
                     /locus_tag="VFF28_07485"
     CDS             complement(20097..20666)
                     /locus_tag="VFF28_07485"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZX45494.1"
                     /translation="MKSRKSSEMEVLLKNSRNLYQDYKNRIIDVILFGSYAKGKFRPD
                     DIDIAVILKDTKEPEILRLSEHFHKYFDINIHLNVVLIENAISNPLFKTLINEGISIL
                     DNKPLHQKIGYESGAIFSINLSKKEKSKKVLFSYALHGKNNQLGILKKLGGKELGRSV
                     FLITIEHVDEFKEFLESWNADFYMMNILS"
     gene            complement(20596..20961)
                     /locus_tag="VFF28_07490"
     CDS             complement(20596..20961)
                     /locus_tag="VFF28_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45495.1"
                     /translation="MSEQNLKDGFFEFLDGGLDEEIKQRYKLAVTAYFKAITQICDLI
                     VLRKRGYCPSSHTDRFRILETHFPKIYLSVDSIFMTYQDTYSIPISKGSCEIVKNEIK
                     KIIRDGGLAEEFKESLSRL"
BASE COUNT         5629 a         4563 c         4223 g         6734 t
ORIGIN      
        1 aatatttcaa catggttttg gaaaatattc attatgacat tctgcgcttc atcatcaagg
       61 ttatgcccag tgacgatttt agtggcagaa gcctcctttg cttttttatt gaggatatat
      121 cttcgaagga tcccgcaaac cctgcatgac ttgtattctt tcacaaggcc cttgatatag
      181 caaagggagt atccaaattc ctccctaaaa gaggtcagat gcagctttat cccattctcg
      241 ctgcagaatc ggacgatgtt ctcccgattg atcttgctgt actcacctat agaaggatct
      301 acatgtatcg cttcaacaga tatgttgcgg ttgccagcta cgatcttctt gatcagataa
      361 agtgcagtag tagaatcctt acccccagag catgcgacaa gtatcttttc ctttttacca
      421 agcagtcccc aaactctgat tgtctttctc acctttttct caaagtagtc taaaaatgcc
      481 ttgttgctga gtttctgccc tgattgggat atgtaaacca ttagtgtttg aaacagcatt
      541 ctgtttttaa gattttccct cccaaaagcc ttatatatca gtaaaatcac tcccgctcta
      601 tggcttacat atggctttac accttgctta gtgtattcat tgtcagcatc atctcactca
      661 taggcatatt gaccttagct ttcagggaat caggtttgaa aaggatcctg cttttcctgg
      721 tgagttttgc agcaggagca ttgcttggtg acgccctgat ccatatcctg cctgaagtga
      781 tagaggaata cgggtttggc ttgaaagttt cactgagcat cctcctgggg atcgtgcttt
      841 tctttatcct ggaaaaattt atccattggc ggcattgcca tgcacctgta ggcaaatcac
      901 atcctcaccc tcttgctttt atgaacatta ttggagatgg catccataat tttattgacg
      961 gcatgctgat agcaggttca tatcttgcct cttttcctct tggcatcacc accacgattg
     1021 cagtgattct tcatgagatt ccccaggaat ttggggattt tggggtgctc cttcatgcag
     1081 gcatgacaaa agcaaaagct ttgttcttta accttttgtc cgccacagca gcgattctcg
     1141 gtgcggttct aactcttatc attggctcaa gctttactaa cctaactgaa tttatcctgc
     1201 ctataactgc aggaggcttc atctacattg cagggactga cttgatccca gaactccata
     1261 agaaatgcga gcctgcttca gaaaatgttt atcagcttat tgcttttatt ctgggcatag
     1321 gtgctatggt cctgcttctg ttatttgagt tttaatggca tacccttctt ggatcgcagc
     1381 ttgagcactc aaactctata gttgtatgct caatatggaa tttctttagc tccgaatgga
     1441 ttctgcacaa tataggataa gtctcatcca ctttcatgtt aggcactgca acatgtgcgc
     1501 tgattgaata attgccgggc ccaagtgacc agatatgcgt gtcatgaaca tccttaacct
     1561 tatctatttt cttgattgaa tcttcaacct tttttacatc aattcctctt ggcactcctt
     1621 caagaaaaat atttactcct tccttgacga tatcccatgc actgtacagc acgattagtc
     1681 cgatgaggat gctcattatt gggtcgataa tataaaaatc agtaaagatg attacaaccc
     1741 ctgagatcaa tactcctatc catcccaaaa aatcctcagt tatatgccag aatatgctgc
     1801 gcatgttgat atccttgtct ttggatttcc acatcctaag tgcgatggct ccatttacta
     1861 tcatgccgac aagcgctata aaaaagacta tcatgccctc aacaggttta ggttcaagta
     1921 tccgcaggta agctctataa aagataaacc ctgtgattaa gatcaatgcg atcgcattta
     1981 tcagcgcagc aaggacagtt gttcttccat acccaaaagt ctttgtttta gtaggcgcag
     2041 cctgcgcctt ttcatttgca taaagtgaga tgccgatggc gatagtatca gaaagatcat
     2101 ggatagcatc gctgataagc gcaaggctgt ttgataaaag tccgccggca agttcgacaa
     2161 gtgtaaagct gccattaagc actattccaa agattagatt cttcttgctc tccctatggt
     2221 catgcatatc tatttcttcc ctttcttgct ttcaaagtcc tctattgcct tatacaggcc
     2281 atcagcactt agattagagc aatgcacctt tatcggtggt agtcctccaa gtgcatcctt
     2341 gatatcatct cttcctatct tctttgcttc ttcaagggtt tttcctctgg caaggtctgt
     2401 tatcatcgaa gaggtggcga tcgcggcaac gcatccaaac gtctttacac ctatatcttc
     2461 tatatactct ttgccttctt tgttcttgcc gaccttgata tagacccaca ttaagtcacc
     2521 gcaattatgg actgctaagc tttcagttaa atatgagtgg cagtcatcta cctctagatt
     2581 acacacatct ccccgatagg gtattttcgc aatttcagtg attggagtat aaatatactc
     2641 attatcaaac caggaatgtg tggcagtgtc tttactgcat ttgaaatcaa tgcatagtat
     2701 ctttgccagg cattcaagcg atgttcgatc cccgatgtgg attctatagg attttctatg
     2761 attaactcct ttaacgatct ttttttcttc ttcataaatt gaaggtgtta tcttttgccg
     2821 tagcaaaaga gttttcattt gttgacataa ctcccttgaa atagttgtat atcctgctct
     2881 tggtcttttt atattgaaat aaccatcccc tttccataac ccatagatta ggcttttctg
     2941 cttttcagca ggaagtagca ttagctcatg aggtatcctt ttgctctggg cacctttccc
     3001 aaacagggtt ttgaaaagtc gggtaatcca aacattattg acttcgacaa tcagggtttt
     3061 ctttttaggt attgtttttg ttttagcttc tatatcaaag agtttcttta cgatgtaggc
     3121 aacattattt cctaactctt gctcctttat attaaatgtg aagcttaaat aggtatttgt
     3181 cactttttct tttaaatagc cttctgccaa ataataacct gcaagcctca aaaaatcatc
     3241 atttaagggt attttgctgg gaatagcctt gctcctaaaa tcccatttct ttttttcatt
     3301 attaaacaat aaatgatctt tatctctgac ttctttactt ataggatacg cgattaagtc
     3361 tttttttgca agctggtcag catgatacca ccctgcaggc aatttttcct ttgttttttt
     3421 atgggagaac caccaggttt ttggaacttt taccgcatag atcatatggt ctggtgttaa
     3481 atagcttgtc cctagcttat tttttatttt gaccaatttg ccagagaaac atttccttga
     3541 aacatcagag attggatggt acaatccatc atggcctaaa acacggttat gcttatttat
     3601 ttcccctatt ttgttaagag aatcattgca atgtatcagc gtttctggtg gcaggcatac
     3661 aggatttccc acctttccta tgccgtcagc atcagctatc tctcgcagat tgtgaggatg
     3721 cttgaaatgt tccataactt tttctgagta catatggatc accttttcca aagggggctt
     3781 atttccctaa gctccttcac cataactttc aggttgtcaa ccgtataatc cagctcttct
     3841 tttgtagtaa accgggataa tgtaaacctt atcgatccat gtgcaagagc atggggcacc
     3901 cctattgcag tcagcacatg agaaggtgtc aagtccctgg atgagcatgc acttcctgtc
     3961 gatacagcta tccctctatc atcaagtttg atgagcatcc cttcaccctc gataaatttg
     4021 aagatgatgt ttgcattgtt gcaaagcctc atccttcttg gcccgttcaa ttttacttca
     4081 gggatctctt tttctacctg attgatgaaa ttatctctta atttttccat ctttttgcat
     4141 tcttgttcgg tgctgagttg cacagccttg gcaaatccga cagcgccagc aacattttca
     4201 gtccctcctc ttaggttaga ttcctgcccg ccgccgtcat taagcttttt caaaatagtc
     4261 cccttcttga catagagggc tccgatacct ttaggcccat gtatcttatg cgcagaaaag
     4321 cttgccatcg taaccttttg cgaaacgaca tctataggaa cttttgtaaa cgactgcaca
     4381 gcatcgatat gaaatgcaac cttgttctca tcgcatatct tgccgatagc tgagatatct
     4441 tgtaatgtcc ctatttcatt gtttccatgg attacagtga caaggagtgt catatctgtt
     4501 atagccgatg acaatttctt aaggtcgaca aatccttctt tatcaacatc cagccatgtc
     4561 actttgaatc cttccttctc aagtgatttg catgtatatt gcactgcgtg atgttctatc
     4621 ttagtggtta tgatatggtt tcctttatct ttgttggcat acacataccc cttgacagca
     4681 agattgtctg actcagtccc gcctgatgta aatattatct catcactttt cgcatttatg
     4741 cgtttagcga taatttccct gctttgttca aggccgcttt tcgcctcttg gccaggagta
     4801 tgcagtgaag atgcattccc atacgtttca gtaaaatacg gcatcattgc ttcaactact
     4861 ctttcatcca cttttgtcgt ggctccatta tcaagataag ctttcatgta aacattcctc
     4921 tctgcagtat ttcctcgcat gcttttcagc aagattaagt attggaagga tagtgtcttt
     4981 attgagctca tataccttaa gtttgcccga gcgcttgaaa tcaacaaagt ggcatttcaa
     5041 aagctttctg aggttatggc tgaccttgct ttgctcttcc tttacgctgg cgcagatctc
     5101 gctcacagac ttggccccag ttgcaagaca ggtaattatc ttcatccttg tcttgccaga
     5161 tatcgtctca aaaaaggatt cgtatgacga gcacttcata tgaagtgtaa gtcatatgta
     5221 ctatttaaat ctttctggac taaagaaaaa gcaccaggaa aatcctactt gattagtcgc
     5281 tgtttgaccc aatcatcggc acttcgtcag aaatatattg gtaagcgctg tatgcagcga
     5341 ctactccttg agcaacagca gtaacagcct gcttgaatct gctgtttatg acatcaccgc
     5401 atgcaaacac ccctttaaca ttggtcttgc cgtcgctgtc aaccattatc tcattctttt
     5461 catttacatt tactcctata tccctgacta tatgagacac tggttccctg cctatatcta
     5521 caaagatggc atcaagcatg agataggtac ttcccttata gggcctgtca agtacaacgc
     5581 tcttcacaaa cttctccccc tttatcccaa caacattagt tttgattatt atctcaacct
     5641 ttttgtttgc ctcgataagg ctgcaatttt tcgggtcagc ccgtattttg tcgccgcggt
     5701 aaatgatata tactttcttt ccaaactggg acagcatcaa agcttccttt gcagcagaat
     5761 cagagccgcc cacgacggat attaccttgt ttttatacaa agctccatcg cagagtgcac
     5821 aataatgtac acctttgttt gtatactgct tctccccagg tacatttagt ttccgccagt
     5881 cagttcctgt tgcaactatg gctgccttgg cttcataact tttctttcca aagaaaacct
     5941 tcaagaattt gccatctttc tctatcttgt cagctttgcc ctcgataagc tcagcgccat
     6001 aagctaaagc atgtttcttt atcctatcag caagctcaat tccaggtatt gattcatatc
     6061 cgggaaaatt tgtcacttca cttgaaagcg ctatagctcc tccggattct cctatgacaa
     6121 tggtctttag attgaatctt ccgcagtaca ctgcagcaga aagccctgag acgccagcac
     6181 caagtattat cacgtcatac attttgctta aagattcaat gcctctttta aagcttgctg
     6241 gtcaaaaccg atgataggat catgtccttg gaccacgatg acaggaactc ccatctggcc
     6301 ggtcttttcg gccatctcat ctcttgcatc atcgtcaaga gaaacatctt tttcgacgaa
     6361 ctttatattg ttgtctttca aaaaatcttt tgctttaaag caccacggac atgttggtgt
     6421 actatacaca gttactgttg gcatgcattt caccccttat aaaacttatt ttttcagttt
     6481 ttatattttt ccaatcttaa gattcagttc ctttgcagct tttgagatat ctccgctttt
     6541 ctggatatag gagccgcaga caaaatagtt ggctccagcc tgttttgcag tgagtaaggt
     6601 taaagggctt atacctccgt ccacctctat gtctgatgtt ggcataatct ccctaagttt
     6661 cctgaccttt tcaagagttt ctggcaaaaa tgggctccca tagtaaccgg gatttacagt
     6721 cattataagt acctcatcca catcatctag cacttctttt atcctttcga taggcgtttt
     6781 gggatttaat gcaaatccta tccttttccc catcgccctg acctgattta ccatcccttt
     6841 aggatccttg catgactcta tatgcgccaa aatggtatct gctttcttcc catgtttaga
     6901 cacccatgat gaagcatctt ctaccataag gtgagcttct acctggcatc tgcattcagg
     6961 cactttaaag ttgaaatcaa tgctgcgtgt aggaacaaat ttaccgtcca tgacatcaag
     7021 ctggatagta gaaaaaaaat ccttaacttt gtcgacccgt tcatccagct ctttctggtt
     7081 gcttgctatt atcgaaggga ctatctcgct tctcataagt cagagcagtt gaaggataca
     7141 ttataaactt tgctataagc tgattttcca aaaaaaagag cccaaagttt actttagctt
     7201 aaatagcttc ggcttgcaca gcaggtcctt ttccgttgtg attatgccgc agatatcgca
     7261 gatatatatc ctcctgttgc actgagggtt gcacagatgt cctatgtttg ttatcttcat
     7321 cccgcaggtc ttgcaaaagg tcatttttaa acgaagctgg caattgatta ataacatatc
     7381 gcttactggt taaagaaaag gttttaaaat taaacatcta cataaagtgg tatggcattc
     7441 acacaaatag cagccgcagg ggaatatatg gattcccttt ttgtcgggat gaggcatttt
     7501 ccgacgaaaa aggttatctt gttgtcaaca cctgaaaatc taggcatagc tgagaaggca
     7561 agaaaggatc ttctaaagtt taagatacct gtatttataa aaaagctcga aggggatcta
     7621 tgggaagctg tatttcaggc agtaaaagag ataaaggagg tcgagggtga gagtgacatc
     7681 ataataaatt cagcctccgg cgattctggg cttagcaggt gtgctatctg ttcagcagcc
     7741 tttgtcaatg gcctaaaagc ttttacagtg gtaaaagatg aagttatcct tatgcctatc
     7801 ttgaaatttt cttattataa gatattgacc gataagaaga tggaaattct aaagtacctt
     7861 ttcagcaacc aggattgttg ctcctcctta gatgagctgg gaaggaagct taagatgtcg
     7921 cttccgttgg tttcttacca tatcaatggc aatctaaaat cagaggggct aaaagagatg
     7981 ggtcttatag ttgctgagga gttcaaggga aaagtcagga taacgcttac attgcttggc
     8041 aggcttatcg tcaagggata tgtaaggtag gccaggattg ctgttggtta attttataaa
     8101 atgtcccagc ttaattttct aaaatggcaa tttggaacgc agtactcagg atatcgatag
     8161 cagttgtcgc cctcattttc ctattctacc tcctgacttt tttggaaaaa aggtttacat
     8221 caaaagtaaa atctctgaat cagttccata atcttaagat attcttccgt ctggttaatt
     8281 attttatctt gctggtaacg ctggtcatat tcttgtttgc ctatgtaggc tcatgggcag
     8341 gccttggtct tggccttggt tttttcacag ctgctctggg ttgggctttg caaaagccga
     8401 ttacaggtat agctgcttgg ctcatcgtga tagcttatcg tccatttaag ataggtgata
     8461 gggtttcgat agggggcgta aggggcgatg tagcagatat aaggctgact cacatttatc
     8521 ttaatgaagt tggggggatt gcagcaggag aagagaattc aaacagggtc atcatgatac
     8581 ctaactctat tctcttcgac cagaatataa tcaactatac taaatcatca ccttatgttc
     8641 ttgacgaggt tggcttttct gtcactttca gctcagacat tgatatcgca aagaagattg
     8701 tccttgattc cactcgaaga ataacccaag aagtgattga gaggataaag caggagccat
     8761 atataaggat agctttgacg actgacgggg taaaattaaa tgtcaggtat atgtctcctg
     8821 ctgtgaggct ctatgagatg agcactaagg ttgcagagga gatagttaga gggataaata
     8881 gtgaatcaaa ggtccagttt gcctaccctc ataccgagct ggtctttggc aataccaaaa
     8941 gcaaaagata gtcgctttga taaggatgcg cttgtttcta tgctaaattg ctcaaacgaa
     9001 acctttatat aatttaaatt ctacacacta gttggttcat ttaggacttt tctggaaagc
     9061 tattttttta ctttttagta aactatccga cgaggtggtt aagatggcca aaggtgagat
     9121 gagcgtaaaa gaggctggca ggaaaggtgg agaggagacc agcaggactc atggcaggca
     9181 gttttatcag gatatcggtc ataaaggtgg ccagattgga ggagaaaaag gcggccgccg
     9241 ggtaaaagag cttattggta aggcaaaaag aggtgagtag catggtaaga ggagagatta
     9301 gcgttcgtga agcaggaaga agaggtggag aggagactag caggacccat ggtaaagagt
     9361 tctaccagga aataggccac aaaggaggcc agcgtggagg tccaagggtt agggagctca
     9421 ttgaaagagg aaaagagtca gaatgaggtg ctaaatatgg ctgaagaaaa aaagggcgag
     9481 atgagcgtaa aagatgctgg aagaaaagga ggagaaaaga caagtcaggc ccacggcaag
     9541 gaattttacg aaaacatagg ccataaggga ggacagaaga caagtcaggc ccacggcaag
     9601 gatttttacg aagatattgg tcataagggt ggggagagga caagtgaatc tcacggcaag
     9661 gatttttacg aagatattgg tcataagggt ggcgaaagga caagcagatc ccatggtaaa
     9721 gagttctacc aggaaatagg ccacaaagga ggccagcgtg taagggagtt gatcaaagaa
     9781 ggcaagaaaa ctgaagaggg gtgattcctt atggctgaaa gcaaaggaga tattagcgtt
     9841 cgtgatgcag gaaaaaaagg cggtcaggag accagccgta cccatggtaa ggaattttac
     9901 caggagatag gaaaaaaagg cggtcaggag accagcaaga ctcatgggag agagttttat
     9961 caagacatcg gaagaaaagg cggcgagaga ggaggtccaa gggttaggga attgattagg
    10021 cgggccaaag gtgaggatta aaaagaggtg aggaatatgg ttagaggtat gagggcaagg
    10081 gcggaaagta agggcgagat aagcgttcgc gaagcaggca gaaggggcgg tcaggagacc
    10141 agccgtaccc atggtaagga attttaccag gagataggcc ataagggagg acaacgggta
    10201 agagaactga tccagcaggg caaaagggca gaaggtgaag agtgatggaa ggccgctctg
    10261 aagaaaaaga gaggtctgcc agagatagag gaggtatgac tgtaaggcaa gcaggccata
    10321 aaggaggcca gcgtgtaagg gagctgatta aggaaggaaa agaaacagaa gagaggtgat
    10381 tatgatggct gaggaatcaa aaggagatat tagtgtgaag gaagctggaa aaaaaggcgg
    10441 agaggagaca agcaggactc acggaaagga attttacgaa gacatcggaa aaaaaggcgg
    10501 agaggagaca agcaggactc atggaaggga gttctatcag gagatagggc ataaaggagg
    10561 ccagcgggta agagaactca ttaaggaagg caagcaggca gaaggtcagg aataggacct
    10621 ttagtccttg gtgacggcaa gcgttaataa tcagtgagat tattgattac attttttagg
    10681 cttctagcat tatcaaagaa atcacgccaa gcatctttct tgggataatt ctctatattg
    10741 aatgaatcag gaaacactat agtaaggtcg tcccactcaa aaggcgcagc gatagtaagc
    10801 cttggctttg ctctggcgcc ataagggcag actgatgtct gcatggcaga attcctgttt
    10861 acatcgatga atatcttatt ttttcttttt gtttttaata ggctggtggt ataaaaattt
    10921 ggatcagata acacaagtga atcagcgacc ttcaaggcaa agtctttgac tttatcgctg
    10981 gtatactctc ttttgatagg tacgctgata tggtatcctt tgcctccggt agtcatgatg
    11041 taaggcttta atcctatgtc tgacaatatc tttctcagtt ttaaggcggt ttccttaagc
    11101 ttttccacag gcacgtcccc agggtcaagg tcaaacacga ttttgtcagg aaccaggggc
    11161 ttgtcatgtt tagatgtagt tatatggggc tctgccacca tattggcaaa atatatgagt
    11221 gatgctttat gggtgataat catatactcg gttttccttc tctcgctttc tatcatcaca
    11281 gtttttatcc aatctggcac cgacccaccc atatttttct ggaagaattt agccccctct
    11341 actccgttgg gaaacctata aagcgataat ggcctgtctg ttatatgcct aagtataaga
    11401 ttagaagcgt cttcataata ctggatataa tcgcctttct ttatatcact gtatattttc
    11461 ttgttctggt ttgtaaggct tacttgcctt ccatctgcat tcgagattcc cttgttctca
    11521 tactctttca tatagctctt tgccctcttt actatccagt tctttccttt tgttctgata
    11581 agatgcagct ctgcctcgac tctgttgccc ttaaggaaaa aatggatatg gcctttttcc
    11641 atggcttgcg gcaagggcac tgcttcttta tcctttatgg aaaggtttgt gtatctccca
    11701 ctgtcccaga tatctactgt tcctgcacca taattgccgg cagggattct tccctcaaaa
    11761 ttaaggtatt ctaccggatg gtcttctgtt tgcaccgcga gtctaacttc ctctgtatag
    11821 tatggcaatc ccttaggtat tgcccaggac ttaaggacgc cttgatattc tagcctaaga
    11881 tcataatgaa gccttcttgc attatgcctt tggattacaa aagaattaac gtccttcttt
    11941 tttattattg atcggggctc aggtgtcctt gcaaaatccc ttttcttcgc gtactctaat
    12001 aaagccataa aaaaagatta ggcgatagcc tcgcccttga attggtcata gtcagccaga
    12061 atctgttctt cttctccttt gacaaggtct ttcttagcat gggcaaaaag ttctctctcc
    12121 tcctcgataa tgtggtgctc gaggttttcc atcagtacct tgaattttgc ccaccatctt
    12181 atttcttcct tttccctttc gatcttgctg agcagctccc tgacaacctc atgctcctcg
    12241 agtgcctcat atgccagctg ttttgaatgt tctttttcca tcagcaaagg atagaggaat
    12301 ttctcctcac catcaagatg tgggataaga ttttcctgta tatccataaa ttttgcttta
    12361 tcttttgtct tcatcgtctc cttcatcata tcctttacca tatcatgctc catcttcaat
    12421 ttatcatata tcgttttcat tttttcacct cttttctttc attttgcatt ctcccaccag
    12481 ctcaatcttc cacctcaagg ccatggtttg caggagcgtt caccaccttt ccataggctg
    12541 tatatcttga gccatggcat gggcagtcaa atgttttctc ggtgctgttc cataaaacac
    12601 cgcatcccat atgcgggcag attccgctga attttcttac ttttccgtca tttatcatca
    12661 cttttttgcc tatcctgccg ccttcaccag gcctgacctc ccggtctgac tttaggtatt
    12721 ttacgaactg tccagcaaca ttcatgttat gtgccaggaa tcttccgacc gcttttttcc
    12781 ttgacgggtc gtaaagcttt tcccaaatat tctttctgcc gatgataaga tcagagataa
    12841 gtattcccgc gattgtgcca tgcgtcattc cctgccctga atcagctgtc gagatgaata
    12901 tgtttttctt gccaggatat cgtccgataa atcccatata gtccaggggt tctatcacct
    12961 gtcctgacca tctgtagtct atgcttgtga tgggaaatct ttcccttgcc catttttcca
    13021 gcctcaaaaa tctgtcttct ccatcatttg cctgtcctgt cttatgatcc tctcccccaa
    13081 ttatgatatg gtcatatctt ctatcgtatt tttggagcct cacatagtga taattctcta
    13141 atgtgtccca ataaagtgcg tctgataaca agcctttctt tatccttgca gcgataacaa
    13201 aagtcctgta cgctgcctgc tttaagacga tttttttgtc cattataggc acgtttgtgg
    13261 cgatgacgat attttttgca tggactcgct ttccatcagc agtcaccacc tttcctgcgc
    13321 ctacctcctg cgcatgagtg ccggtaaaga tttcccctcc tttcctctta atggtgcttg
    13381 caagcccgct cagatatttc atcggatgga tatgcgcctg tttagggaac ctaaggtttg
    13441 cacccatatc aaaagtgttg tcaggtgcag tgtcgcttag tttaggctta aatcctgctt
    13501 ttttgtatgc ctttagctct ttttctagta tcttgccatg ttcagaaaac agataaccat
    13561 caaccccagt atagtcgcag tctatcttct cttttttaga gatattatac attgtctcta
    13621 cggcagcagc atggctttgt gctgcaatca tcgcccctcg ctctccaaat atcttttcaa
    13681 tttcaaacag cctgtcatcc atggtgtagg ataggtgcgc cgttgtcctt cctgtctcac
    13741 cgcttccaag cagcccatct tctagcacaa caactttctt tcccttttca agcaggttat
    13801 atgctgtcga taatcctgca atccctgctc ctatcaccag agtatctgtc ttgatatcct
    13861 tatcaagggt ctgcttgttg cctctattcg ctgaaagcca tagtgtatca caagcccctg
    13921 atgttactgc ctcccttccc ttcatgcttt ttagcgcaga cagtttatcc attggctttt
    13981 ctctcctccg ttagggtgaa gcatctatcc tgtatgtaca aagattcctc agatgcaaga
    14041 aacacatagc ttggcgcaac ttcgataggc tggcctgctc tgccaatccc gctgtctccg
    14101 cctgttatga gcgcaaattt gcctttgagt gccgcataac tgtcgctgtc atatatcggc
    14161 ctcggattca tcttccattc agcacccggc tgcttttcct ggtgctgccc gggaagttca
    14221 ctaggaaagt ccattttaac gatcacctca aaagcttaga attagagaat attctgtttt
    14281 atcagtattt aatcatttat tgctataatg gggtggtcaa aaatgacaag taggaaaaaa
    14341 aggttactcc ttaagcacca gctcagcatg catagcatca ttgtctattt tacttatggt
    14401 aaaacccctt ctttcaaaca tatgcttcat cactgagttc tcttttagaa agtcagcgta
    14461 gatcttcttg atgcatcttt catgcgcaat ctccagcaca tagtcgataa atctgtttcc
    14521 tatcccttgc ttttgccaag ggtctgcgac aacgattgca aactctgcag tctcgttata
    14581 agggtctgca ataagcctga caacgccggc catcttcttc tttccttctt cctctacctc
    14641 agcgactatg gctatttctc tgtcataatc gatttgggta tacctcacta ggatctcgtg
    14701 gtttatctcc ttgataagct ggaaaaatct gaacctttgc gtctgctctg agaaattggt
    14761 gaacatctca gcttccattg gttcgtcttc aggtcttatc ggccgaagta tcgcttcctt
    14821 accgttgctc atcttatact ttgatatata ctcctttgga taaggggaga tgaccaggtg
    14881 tgaatatggc cttgggttct ttcctgcaat ttcaggatca agtattatct tggcatcaag
    14941 gactatccct cccttttcgt cgactgcaaa agggtttatg tctatttcct ttatttccgg
    15001 gaaatccatg acaagatagg caaatttata caaaaggaat tggatagatg cgatatctgc
    15061 tccctgcatc cctctatatc ctttcagtag ggtgtatatt ttggtttctt ctatgagcct
    15121 gagagcaagc gccatattta gtggaggaag cccgatgttt gtgtctttga atacttcgac
    15181 agctactccc cccatcccaa agacgatgac tggcccaaag atagggtcct tcttgcagcc
    15241 gatcaaaagc tcgtattttc tggaggtcat cttttcgata aatatgccat ggatgtcagc
    15301 ctttggagca tgcttctttg ctgaagtcat tatctctttg aatgcttctt ttacatgctc
    15361 ttttgattgg atatttacct ttacccctcc aacatctgtc ttatgcagta tatcaggaga
    15421 aagtattttc atgaccacag gaaatcctat gtcagaggct gtctctacag cctcagcctc
    15481 tgtttttgca atcccttttt tagctatggg gatatcatag tttgaaagca tctgttttgc
    15541 ttcagattca gtcatcacac ttcttccctc gcttactacc tttgcgatta tcttttcatt
    15601 ttcctgtgtt tttggcttga atgcatgagg tatggtggat ggagtctcat aaaggagctc
    15661 aaggtttttg gaatagttga acatctccat aaagcattcg acagcatcct cagggacaga
    15721 atagacaggt atgtttccgt tcttcagtat cttcctgcct tcagttacat catcttcacc
    15781 catccatgca gtaaggattg ttttgtttga tgtctttgag agatctacta tctgttttgc
    15841 aatagttgtc gcatcagtca tagcctgagg tgtaagtatt acaagcagtc catctacatt
    15901 tggatcccgc aggcaaacct ctgcagcctt tttgtatctt tcagggtcag catctccaag
    15961 cacatcgaca gggtttgact tgctccatgc ttcaggcagg caagcattaa gtgatttcat
    16021 gctttcaggt gaaatgctgg caagggttcc tccttttgag attattgaat cggttgcgat
    16081 aactcctggg ccgcctgcat tcgtgactat cgcaagccgc tttcccatag gcctaggctg
    16141 catggcaagg gtttgtgcgc agttaaaaag ctggcctatc gtatttactc taatagcgcc
    16201 tgctcttttg aatgcagcgt caaacacagc atcatttccg gttattgatc ctgtatggga
    16261 catggctgcc tttgctcctt cagagctttt tccgaccttt atgatgatga ttggttttgt
    16321 tctggcaaat gccctggcag cagataagaa tttctttgca taggtaagtg attccatata
    16381 tatgaggatc gagctggtct caggatcctg gccaaaataa tcgataaggt catgaaatcc
    16441 tacatcgatc atcgagccta tggagacaaa atagctaaag cctacatttt ggcttacgct
    16501 ccagtcaagg acagctgtcc ctagcgctcc tgattgtgat atgaatgcga tctttccagg
    16561 aagcgccatc ttgtttgcaa agcttgcgtt aagtttaagc ttaggcctga taaatccaag
    16621 acagttaggc cctattatcc tcatatcata ttttcgggca gtcattgcta tctcatcgca
    16681 cattgcttcc cctttctttc ctatctcctt gaacccagca gatattatga cgatgccatg
    16741 tactccgctt gccccgcatt cttcaactat ggcaggaaca gtatctgcag gtgtagctat
    16801 gattgcaagg tctatcttgt caggcgtttt gcttatagac gggtatgttt ttaccccaag
    16861 gatgcttctt cttttaggat tgattggata tactattccc tcaaagccgc tggcgataag
    16921 atttttcatc agcgcatgcc ccacgcttcc agccttgttt gtagctccga taactgctat
    16981 tgttgtagga ttgaatagtt tctcgagttt atgtaaagcc atctttcctc cttataattt
    17041 ccagtatggc gataggtttt tctcaaggct cagaattcga ccttcatact cgtccctggc
    17101 gatgatcctt gaatctgtcg cttgttcgtc aaatctcatc ttgttgccgt ttgctccatc
    17161 tatgaaatta aatatgcatt tttgtaaaac cgataggtta taccccccct caagagttgc
    17221 gaatacgttt ttaaatcttt ggcttaggat atggcctatc ttataatatg aacggcatga
    17281 tagcctgagg tcaagaagca ggtctgcaga atgcccgtca aaacctgcag atacagccac
    17341 cacatcaggt ttgaactgtg caggtattat catcagcctt tccaaagcat caataaatag
    17401 gtcatctcct gttcctggcg gaagaggcat atttatagta tacccctttc cagttccttc
    17461 gcctatctca tcgacatttc caccgcccgg aaaagcaggc ccttggtgga gtgaccaata
    17521 tagtaccttg tcagtattgt agaatatctt gacagtcccg tcgcccagat gcccgtcaaa
    17581 gtcaaggata aatacttttt ttccttcatt cacaagcttc tttgcagcta tcgctatgtt
    17641 gttaaagatg caaaaaccgc ttgaatggtc aggatatgca tgatgtccag gtggccttac
    17701 cagtgcaaaa tcatttgtct ttgaagccat tattgtagcg cccacagcaa gcacagcagc
    17761 ctcatagctt ctttcagaga ctattgtatc tggatcaaga tgccctcctc ttttgcaggt
    17821 atccttgaca agatcgatgt actcctttgt atgtataagg ccaaggtatg gctctccgct
    17881 ctctatcttt gtctccttaa gtttccccag tgattcaagc cttttcctgt tctcaggatg
    17941 catgccagtg tcatgctcta gaaagatgct gttgtagatt atatccatca ataatggttt
    18001 atagatgttt atttaaaaat ctttttcagg gtcacctata tacacttatc catcgccata
    18061 ttgcttctgc attttgggca agtcatcttt tcgcagggag tcccttttac atggacgact
    18121 tttgtgccgc attttgggca tatgcaatta gcaggcacag tgcaatgctc atcgggtatc
    18181 atgaaccttt gctatgctct cggtctattt atacatattg tttccgcacg ccatcatatt
    18241 ggcttttcta tatagaaaaa acaaggcaaa ggtcggtttc ctttgccttg aaaagaggtg
    18301 tttagtatga ttagacagct cgtggtaaat gaggaagggg gtaaccacga gccatcaata
    18361 atcaatttga ctatctgttt aattgatagg ggtttattaa taccttatga acagctggta
    18421 aagaataaaa atccataaag tttataaatg aaaaaaatgg cccttgttct atggtgtatg
    18481 tagtaagccc agaatggttc tatggtatag actatctgtt tgattctgtt tcagttatcg
    18541 ttgcattgct gataaccttt ttctcctata agtcgtataa atacacctcg caaaaaaaat
    18601 acctctattt tgcactttca ttattccttg ttgcagcagc attcctggca aagatattgg
    18661 ctactatccc tatctactcc cgccagctca aggtcgagac gataggtctt gtcacagtca
    18721 cttctcaagt tataagcaag gtcacttgga ttaaccaatt tggagtctcc atcggccgtt
    18781 ttttgatgct tatcgcattt ctcatcttga taattatcac tttaaaattg gctgacaaaa
    18841 agataatcgg gctgcttatc tatttcctgg tgatctcgac cacgcttgta agcgcttcat
    18901 atgaggtatt ccaccttacc cttcttatca tggtcaccct tctctttatg aattataggc
    18961 ataacttcct aaaaataaaa agcaaaaatt caaagcttgt catgcttact ttcttttgtc
    19021 ttatgataag ccaatttttc ttttttggcg caggttttat cagtaatctt tatgtcatag
    19081 gtgaatctat acagttggta ggctatctaa tattgcttgg ggcattcgta aaaatagtca
    19141 caaaaaaatg agcatccgca gaacaaagat cgagataata cacgacatcc ttaactccat
    19201 ccaaaaaaaa ggggggagaa ttaagccgac ccatcttctt tacaagtcaa atctttctta
    19261 taagaagatg cttgagtatt tagaagagct taaaggcaaa ggcatggtgc aggaagaaga
    19321 ggaggaagga aaaaggatgt acctcatcac agataaaggc gcagaataca tccaggaata
    19381 caagaagatc aaggaattct cagacagctt cgggttgtag tttcagtcac ttttttatac
    19441 ttcttatctc aatttactcc tatgtggcgc acaaaggtaa ggcttaagca cagttgcctt
    19501 tttggagaca actgtaagga agcaaaagtt acctgcctga atatgtcttt taacactttc
    19561 aagaaaggtg attcttattt tgtctttcat atggggacag tctttggcga caattacacg
    19621 aaattccttg ctttattgaa gaaagacaga agggttgatt atatagagtc tgatgggaga
    19681 acatttgtgg ttttggaaaa aaggcctgaa aaagagattc ccggaatggt aatcaacaat
    19741 gaggttattt atgtaaaacc agtaaaggtt gacgaggacg gctatgagac atgggagctt
    19801 gctgcaattg aaaaagaaaa actaatggac ttcatcaacc actttaaaga ttcagagata
    19861 cttaacatcg agcagacaaa gctcaaggat atttactttc caaggctcag cccaaacttc
    19921 tcccagcacc aaagggatgc gctggagctt gccatagaag aaggttatta ccagtttcca
    19981 aaaaagactg acctaaacaa gctaagcaag atagcaagac tgtcaaaatc cacatttaga
    20041 gagcacctta aaagagctga gatgaaggtt ttgggagagc tgggtagata agaccttcaa
    20101 cttagaatat tcatcatata aaaatcggca ttccaggatt caaggaattc tttaaattca
    20161 tcaacgtgtt ctatagtaat taaaaaaaca gatctcccta actcctttcc acctaacttc
    20221 tttaatatcc ccaactgatt atttttacca tgaagggcat atgagaacaa gactttcttg
    20281 cttttctctt ttttcgatag attgatacta aagattgctc cagattcata gcctattttt
    20341 tgatgcaggg gcttattgtc caatatagag attccctcat taattaaagt cttgaataaa
    20401 ggatttgaaa tagcattttc aataaggact acgtttaaat gtatattaat gtcaaaatac
    20461 ttatggaaat gctcactcaa tcttaatatt tctggctctt tagtgtcttt aagaatcaca
    20521 gcaatatcta tatcatcagg cctaaattta cctttggcat aagaaccaaa taatataaca
    20581 tcgattattc tgtttttata gtcttgataa agattccttg aattcttcag caagacctcc
    20641 atctcggatg attttcttga tttcattttt cactatctca catgatcctt tacttatagg
    20701 tatagagtat gtatcttgat aggtcatgaa tatactgtcg accgataagt atatctttgg
    20761 aaaatgagtt tccagtattc taaacctatc tgtatggctt gaagggcagt aacctctttt
    20821 tcgaaggact atcaagtcgc agatttgagt tatagccttg aaataagcgg ttactgcaag
    20881 cttatacctt tgcttgatct cttcatcaag acctccatca agaaattcaa aaaatccatc
    20941 ttttagattt tgctcactca ttcgctacct tggtaaattt ggcattattt aaagctttcc
    21001 tttttaacat ggtaaaaata aaggtatttt tatacttatt ttttaacatg gaaaagaata
    21061 cctttcatga gcggtaaaaa atatatatta taacctaatc tcgccatttg aataaattta
    21121 atagggtcac gtttgtttta tatttttgt
//
LOCUS       DASRUK010000172         7306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2168566, whole genome shotgun sequence.
ACCESSION   DASRUK010000172 DASRUK010000000
VERSION     DASRUK010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 7306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7306
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2168566"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..834
                     /locus_tag="VFF28_07495"
     CDS             226..834
                     /locus_tag="VFF28_07495"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HZX45496.1"
                     /translation="MMITTDEMKVLEKSSGIPMIKLMENAGREAVNIIKSRFTDIKNK
                     RILIVTYHGNNGGDGFVAARYLSEEAETDVLFIGDEDRFKEEARINFEKMEKESVQLL
                     TDPEVIDFDEYDIIIDAILGTGSKGELKEPMPYVIDQINSSKAFKVSLDIPSGLHPET
                     GQEAEKVVRADMIIAFHDLKPGLQELKDKVVIADIGLNLVKE"
     gene            882..1682
                     /locus_tag="VFF28_07500"
     CDS             882..1682
                     /locus_tag="VFF28_07500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HZX45497.1"
                     /translation="MNYISKKDIVIPKRKEQSHKGDNGKVLVIGGSRDYVGAPALAGL
                     SALRSGVDWVTIAAPEKVGYAINALSPDLVVKKYKGDDFCANRAKDILNFESGFDAVL
                     IGNGIGVHCKAFINKYIKETKKPLVIDADALRQIKIQDVKNAILTPHRQEFIALLDNS
                     GLTENSFRKRLDNNVIILKGKIDQIITRSKTYYNKTGNAGMTKAGTGDVLAGLCLGFL
                     GQHLSLTQSAINAAYFNGLAGDILLKKKKGFTYLASDLVEEIKRIIKP"
     gene            1872..2015
                     /locus_tag="VFF28_07505"
     CDS             1872..2015
                     /locus_tag="VFF28_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45498.1"
                     /translation="MVRIQKLYSGQLVITLPKRLAEYEGLKKGMKINFRKHKEGFLLE
                     VVR"
     gene            2012..3769
                     /locus_tag="VFF28_07510"
     CDS             2012..3769
                     /locus_tag="VFF28_07510"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZX45499.1"
                     /translation="MNILPEQFFVSADLNSAFFQLGTVIIIATVFAFLAGKLKQPLIP
                     AYILTGLLLGPIYHLITNKELILSLSEIGIAFLLFIVGLEMDLKKLKSIAAVAGLGAT
                     IRSLLMFTLGFIIALFMGFMTKEAIYFGIIIAFSSTMIVVKLISDKKELDTLHGRIMV
                     GILVMEDILAILALSLMTSLDSRSVIAFIMPVIKGLLLFGAALLLTRYVFNPVIKASA
                     KSQELLFLTSVSACFLMSILAYFLNFSIAIGAFLAGLSLAYLPYNFAIIGKVSSLRDF
                     FATLFFVSLGMQLVVTNIKELIVPLAILLVLVVAIKPFITMFICSFFGYKKRVSFMTS
                     ISLAQISEFALILVSQGLILGHISQTLFTLTVLVTIVTMLLTSYFIQFDYSLYTRLQN
                     LLKPFEFFSGKGKDIEYMPKKTRIDAIICGHNRIGYSITRTLAKLKKRTLVIDYNPGI
                     IKELAEKKVSCIYGDVADDEVMERLPLKDIKLLVSTIPSSRVNLLIIRKVKDVNNKAL
                     IFTTATSIEKALMLYDAGADYVILPHFLGGEHVSLLIQRFSSNEDKVIKTKLEHINEL
                     KLRQSIGHEHPRHEGQDGV"
     gene            4034..5476
                     /locus_tag="VFF28_07515"
     CDS             4034..5476
                     /locus_tag="VFF28_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45500.1"
                     /translation="MPEPQEFLKQIGSGKEDVDSPRSGTDIPNGFPEPIRKYRLFVEG
                     YNISIEEPYFWVLHYLRWFWSFVRIDKITDTFSASENSSFFGTSQQRVGLQQDKVSQY
                     LATIGKMIKELFQLVRELRIIDERLGYYSDSYDSYSRSRESAEITLKGIWVDMVEQGA
                     KNPASVYGMAREVQFTTLPDLFFSTHPLRQEDVDIVVERERSEFNRKVREVLKRKLRS
                     YLAWKESTFEEMKNRRIFTIKYLRQHYEIIRMYMSWVKPYLKNIQRLMMDQKKADSPD
                     MLVAFETSMMEVEILAKKEKGKKIELGGKQTQVWQVILVHYDFRTRPEMSYVQEGYQK
                     GPLHLGRVEITFRAYAWTDREIELYKKMREDEDFRLLSLIDGSVKVAMESLGDELMRY
                     LAEAGEDMGKRKEKKQEKPPTITSSLYKGFREMFKGYKQVKKPELPRAPKRSEKFELE
                     QSKKDTAADVKRAMWGIYHHFKKHHGMLNW"
     gene            5743..>7306
                     /locus_tag="VFF28_07520"
     CDS             5743..>7306
                     /locus_tag="VFF28_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45501.1"
                     /translation="MEFRYYTHHPMVDYANSMDPGAYDKGPQKPSTSIKDIGMSVPLG
                     ISAQNVAGIYSKIRMGAGSIELAFPTQGTGNRNAHTPEMYGTDQRQAIRELANINEVK
                     LTTHAAYNVMGMMGRDQRDNFSNTNAREGMREVERAIDFAADTAGAGSVVVHTGEWER
                     PLTEMYLDDPTTKTNLAYDDEGRLMFKRTQQQVFDAQFTLLDDRTSQKMETAQKDRKI
                     SIPIWNRADRDYDGKYQEDIHVMHDKEKWMEKYVGKPAHIKKGDYIDYEGRKIIDPYN
                     VKYGRVPDFDPDARTFKVEYQNWNYFIKEAQEQTNFNKAHWKDKTNKDWRDDPMWYYD
                     GERHPDEAFMHATLETNEGHSRGWAIQFAIDSKNDLELLKKYEEARKYYQQLDKEIPE
                     EEKWKLMRNKEFGDLIPPESKHPLELIEERLNQVRARLTYAKESSASQLQQAEDTAET
                     KEHIVTPSKRFKLHAARFYAEAGIRAMQRTKDPNTPVFIAIENIFPERFGGHPEELKF
                     VIDQSRKWMVDFL"
BASE COUNT         2416 a         1492 c         1671 g         1727 t
ORIGIN      
        1 aaaaggttga gttgtggcag accctcaagg ggcttgagat tcgcaaagaa ggagtgacct
       61 atggcacaat cacagattat tgggacaggt ttgagaaaaa tgtatagttt agctgtcgca
      121 tttcagagat gttatagaac aaggagggta taaaaaccaa ggaagagata gaaaccttaa
      181 tatattcctt cgataacaac attcatgcta aagaaagatt aagggatgat gataactact
      241 gatgagatga aagttctgga aaaaagctct ggcattccta tgataaagct catggaaaat
      301 gctggcagag aggcagtaaa tataattaag tccaggttta cagacataaa aaacaagcgc
      361 atccttattg tgacgtacca tggcaataac ggaggcgacg gctttgtcgc agcaaggtat
      421 ctttctgaag aggcagagac agacgttctg ttcatcggcg atgaggatag attcaaagag
      481 gaagcaagga taaacttcga gaagatggaa aaggagtccg tccagcttct tacagaccca
      541 gaagtcatcg actttgatga atatgatata attatcgatg cgattcttgg cacaggctca
      601 aaaggcgaac ttaaagagcc tatgccctat gtaatcgacc agataaatag ctcaaaggca
      661 ttcaaggtct ctttggacat tccatctggc ctacaccctg aaacaggcca ggaagcagaa
      721 aaggtcgtca gggcagacat gataatcgct ttccatgacc taaaaccagg gttgcaggaa
      781 ttaaaagata aagttgtcat agcagacatc ggactaaatt tagtaaagga atagaatcga
      841 ctacagaaac cattaaatat ccttctaacc attaacctcc tatgaactac atttctaaaa
      901 aagacatagt catacccaaa aggaaggaac aaagccacaa aggagacaac ggcaaagtcc
      961 ttgtcatcgg cggctcaagg gattatgttg gagcaccagc cttggcaggc ctttcagccc
     1021 tgcgctcagg cgtagactgg gtgacgatag cagcgccgga gaaagttggc tatgctatca
     1081 acgctttatc accagacctt gtcgtaaaaa aatacaaagg tgatgacttc tgcgcaaata
     1141 gagcaaaaga tatcttgaac tttgaatcag gttttgatgc agtgttgatt ggaaacggta
     1201 ttggagtaca ttgcaaggct ttcataaata aatatataaa ggagacaaaa aagcccttgg
     1261 tgatagatgc agacgcttta aggcagataa agattcagga tgtaaagaac gcgatcctca
     1321 ctccgcatag acaagaattc atcgctcttc ttgataactc cggccttact gaaaacagct
     1381 tcagaaagcg tctggataat aatgtgatca tcctaaaagg aaagatagac cagataataa
     1441 caagatcaaa aacatattat aacaagacag gcaatgcagg catgacaaaa gcaggcacgg
     1501 gtgatgttct tgcaggccta tgtctaggtt ttcttggcca gcatctttca ttgactcaat
     1561 cagccataaa cgcagcgtac tttaacggcc ttgccggcga catccttcta aagaaaaaga
     1621 aagggtttac ttatcttgcc tctgatctgg tggaagagat aaagagaata atcaagccct
     1681 gatttctagt ccgctggaaa tttttgcttt acggacacaa ctttatcctt ttcagtagga
     1741 taaatatatt taacgagcat tatgcctaca ctaaagccac aaccccaacc tttaaatact
     1801 agtaacagcg atagcttatt aaccaataaa taattagtga aaaaaaccat aaaaaatact
     1861 agtattttac aatggtaagg atccaaaagt tatattcagg ccagctcgtc ataacccttc
     1921 caaaaaggct ggctgaatat gaaggcctaa agaaaggcat gaagataaac tttagaaagc
     1981 acaaggaagg ctttctcctg gaggttgtaa gatgaacatc ctcccagagc agttctttgt
     2041 ctctgccgat ttaaacagcg cattcttcca gttaggtacg gtcataatca tagcgacggt
     2101 atttgctttc cttgcaggaa aactaaagca gccgcttatc cctgcatata tacttacagg
     2161 cttgcttctt ggccctatct atcatctgat aacaaacaaa gagcttatcc taagcctctc
     2221 agagataggc attgccttcc tgttgttcat cgtcggcttg gagatggact taaagaagct
     2281 caaaagcatc gcagcagtag caggccttgg agcgaccata cgaagccttc ttatgtttac
     2341 cttgggattt atcatcgcct tgttcatggg tttcatgaca aaagaagcca tctattttgg
     2401 tatcataata gccttcagct caaccatgat tgttgtcaag ctcatatcgg ataaaaaaga
     2461 gcttgacaca cttcatggaa ggataatggt aggcatactt gtgatggaag atatccttgc
     2521 catccttgca ctgtcattga tgacatccct tgacagcaga tcagttattg cattcatcat
     2581 gccagtcatc aaagggctgt tgttgtttgg cgcagctttg ctccttaccc ggtatgtatt
     2641 caaccctgtt ataaaagcat ctgcaaaatc ccaggagcta ttgttcctga cctctgtctc
     2701 tgcatgtttt ctcatgtcga tacttgccta tttcctaaac ttctctattg cgataggagc
     2761 cttcctagct ggcttaagcc ttgcttacct cccatataac tttgcgataa tcggcaaagt
     2821 aagctcgctg agggatttct ttgcgacgct attctttgtc tccctcggga tgcagttagt
     2881 ggtcacaaac ataaaggaat tgatcgttcc cctggcgata ctcctggtat tggtggtggc
     2941 gataaagccg ttcatcacca tgttcatctg ctcatttttc ggctacaaaa aaagagtaag
     3001 ctttatgaca tcgataagcc ttgcccagat atcagagttt gcgctcatcc tcgtcagcca
     3061 gggcctgatc ctaggccaca tctcacagac cctgtttacc ttgacagtgc ttgtcactat
     3121 agttaccatg cttctcacct cttactttat ccagtttgac tacagcctgt acacaagact
     3181 gcaaaatctt ctcaagccat tcgagttttt ttcaggcaag ggaaaggata tagaatatat
     3241 gccaaaaaag accaggatag acgccataat ctgcggccat aacaggatag gttacagtat
     3301 tacaaggacc cttgcaaagt tgaaaaagag aaccctggtg atagactaca atccagggat
     3361 aataaaagaa ctggcagaaa aaaaggtatc ctgcatatac ggcgacgttg ctgacgacga
     3421 ggtaatggaa aggctgccgc taaaagatat aaaactcctc gtttcgacta ttccaagcag
     3481 cagggtcaat cttcttataa tccggaaagt aaaagatgta aataataaag cactcatctt
     3541 tacaacagca acatccatag aaaaagcgct tatgctatat gatgcaggtg cagattatgt
     3601 tatccttcct catttcctgg gaggagagca tgtctctctc ctcattcaga gatttagctc
     3661 aaatgaagac aaggtgataa agacaaagct tgagcatatc aatgaactca agctcaggca
     3721 gagcataggc catgaacacc caaggcacga gggacaagat ggagtataac tggttaggca
     3781 aaaaaaagtg cacgattagt tcagcgtctc aaagccaaca atatggcgag gagggttcat
     3841 tgactgaaag ccaacaatgc cgagacaaac caggggttgg cccctccggg gtggtttgta
     3901 gccttgcaga gcaaggcgtc gaaggcataa ttgtttcgaa actaatcaaa gcaccatcaa
     3961 cttatcaacc aataactctc caaaatattt atgagggaat aaacttataa atcaaggaga
     4021 taaaaacaac aaaatgccag aaccccagga attcctcaaa cagataggca gtggaaaaga
     4081 ggatgtagac agtccaaggt caggtacaga tatccctaac ggatttcctg agcccataag
     4141 aaaataccgc ctttttgtcg aaggatataa tatttcgatc gaagagcctt acttctgggt
     4201 tttgcactat ctaagatggt tctggtcatt tgtaaggata gataagataa cagacacatt
     4261 ctcagcatct gagaactcat ctttctttgg gacttcacag caaagggtag gcttgcagca
     4321 ggataaagtg tctcagtatc ttgctactat aggaaagatg ataaaagagc ttttccagct
     4381 tgtaagggaa ctcaggatta tcgacgagcg cctcggctat tactcagact cgtatgactc
     4441 ttattcgcgt agcagggaaa gcgcagagat taccctcaaa ggcatttggg tggacatggt
     4501 agaacaagga gcaaaaaatc cagcctctgt ctatggcatg gctagagaag tccagttcac
     4561 aacattgcca gacctattct tcagcactca tccattaagg caggaagatg tcgatatagt
     4621 agtcgaaaga gagcgctcgg agttcaacag aaaagtaaga gaagtcctaa agagaaagtt
     4681 aaggtcctac cttgcctgga aggaatcgac atttgaagag atgaagaaca ggcgcatttt
     4741 tactattaag tatcttaggc agcattatga gattatccgt atgtatatgt catgggtaaa
     4801 gccttatctg aaaaatatcc aaaggctcat gatggaccaa aaaaaggctg acagtcctga
     4861 tatgctcgtt gcctttgaaa cctccatgat ggaagtggag attttggcaa aaaaagagaa
     4921 aggaaaaaag attgagcttg gaggaaaaca gacccaggta tggcaggtca tccttgtgca
     4981 ttatgatttc aggacaaggc ctgagatgag ctacgtccag gaaggctatc agaaaggccc
     5041 gctgcatctt ggaagagttg agataacgtt cagggcatat gcatggacag acagggagat
     5101 agagctttac aaaaagatgc gggaagatga ggatttcagg ctccttagct tgatagacgg
     5161 ctctgttaaa gttgccatgg aaagtttggg agacgagctc atgagatacc tcgcggaagc
     5221 aggcgaagac atgggaaaaa gaaaagagaa aaagcaggag aagccgccca cgataacatc
     5281 atccctctac aaaggattca gggagatgtt caaaggctac aagcaggtca aaaaaccaga
     5341 gcttccaaga gccccaaaga gatcagagaa gtttgaattg gaacaatcaa agaaggacac
     5401 agccgcagat gtaaagagag ctatgtgggg catctatcat cactttaaaa agcatcacgg
     5461 gatgttaaat tggtaaataa ggaggtatag ttaagaaagc taacgaataa agccaacaat
     5521 gacagcttca gcgccttgca ttggaatatt tggatgtcag accaacaata atggcttcga
     5581 cggtttgctt ggcgcaaaaa cctccgagca acggaaaact gcggttttgt tccgctcgtt
     5641 tcactcggga aaccacccca tagggggcga cccctgtctc ggctcgtcat cgttgactta
     5701 caaaaaaagg tgatatgtat ggaagaggaa aacaactaaa aaatggaatt cagatattac
     5761 acgcaccatc cgatggtaga ttatgctaac tcgatggacc ctggagcata tgacaaaggg
     5821 ccgcagaagc cctccacgtc gataaaggac ataggtatga gtgttccttt aggaatctca
     5881 gcgcagaatg tagctggaat ttattcaaag ataaggatgg gtgctggctc aatagagctt
     5941 gcctttccca cacaaggtac aggcaacaga aatgcccaca ctccagagat gtatggcaca
     6001 gaccaaaggc aggcgataag agagcttgca aacataaacg aagtgaagct gacaacccac
     6061 gcagcataca atgtcatggg catgatgggc agggaccaaa gggataattt ctcgaacacc
     6121 aatgcaaggg aaggcatgag ggaagtagag agggcaatag actttgcagc agacacagca
     6181 ggcgcaggct ctgtcgtcgt gcatacagga gaatgggaaa ggccgcttac tgagatgtat
     6241 cttgacgatc ctactacaaa gaccaatctt gcctatgatg acgaaggaag gctcatgttc
     6301 aaaagaacac agcagcaggt atttgacgca cagtttaccc tgcttgatga caggacaagc
     6361 caaaaaatgg aaactgccca gaaggatagg aagatttcaa tccctatctg gaacagggca
     6421 gacagggatt atgacggcaa atatcaggaa gacattcatg tcatgcatga caaggaaaaa
     6481 tggatggaaa aatatgttgg aaagccagcg catatcaaaa aaggagatta cattgattat
     6541 gaaggcagga agataataga tccctacaac gtaaaatacg gaagggtccc agattttgat
     6601 cctgatgcaa ggacatttaa ggttgaatac cagaattgga actacttcat caaggaagcg
     6661 caggagcaga ctaatttcaa caaagcgcat tggaaggata aaacgaacaa agattggcgg
     6721 gatgatccta tgtggtatta tgacggcgaa aggcatcctg atgaagcttt catgcacgct
     6781 acccttgaga caaacgaagg ccactcaaga ggatgggcta tccagtttgc cattgattct
     6841 aaaaacgacc ttgagctgct aaaaaaatat gaggaagcaa gaaaatatta tcagcagctg
     6901 gataaggaaa tacccgaaga agaaaaatgg aagctgatgc gaaacaagga gtttggcgat
     6961 cttatccctc ctgaatcaaa gcatccgctt gagttgatag aggaaaggct aaaccaggtc
     7021 agagcaaggc taacctatgc aaaagaatcc tctgcatccc agctccagca ggcagaggac
     7081 accgcagaga caaaagagca tatagtgacc ccttcaaaaa ggttcaagct ccatgcagca
     7141 agattttatg ctgaggcagg catcagggcc atgcagagga caaaggatcc aaacactcct
     7201 gtctttatag ctatagaaaa catatttcct gaaaggttcg gcggccatcc tgaggagctt
     7261 aaattcgtga tagaccagtc caggaaatgg atggtcgatt tcctca
//
LOCUS       DASRUK010000173         3142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_806558, whole genome shotgun sequence.
ACCESSION   DASRUK010000173 DASRUK010000000
VERSION     DASRUK010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806558"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="VFF28_07525"
     CDS             complement(<1..907)
                     /locus_tag="VFF28_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45502.1"
                     /translation="MINKRLVLLCLVVLACAFSASAVDFSGYAYNVSEDALNNTNVTV
                     GVYIMQEGQPPTLYAAYSNLSNATGYWKVVTNLTPEDLAGKMIKFTPKKFSAAGDATH
                     VGSNLPHLPYQELAAMLNISDIKFYLKEGATLNVTAVNASGDAVSFKYMVKDTKLGYD
                     IESEWTTAVTQKTIYIPSDRNYSVEIFPQQSMPIYYDVSNLSSYTDPKHVNIVFNVSM
                     TLSRVSGRFNLTNGTGAFDELNIINYLFEPGNMVYSEMAMPYNMSGWFGGTDTYSAAD
                     GTYNISLPATAMSSRVLMMALARSGG"
     gene            complement(988..1434)
                     /locus_tag="VFF28_07530"
     CDS             complement(988..1434)
                     /locus_tag="VFF28_07530"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZX45503.1"
                     /translation="MAFDETDKKLINALLLNSKLSYRELAKEVNVSVATAMHRVKRLE
                     NEKVIKRYTTIIDYDKAGFDVEVLVEMRISKGKLFQVEKEIANHPNVFAVYDLTGDFD
                     AAILARFQNRKAMDSFLKKIQTYDFVERTNTRLILNTIKEKEIGLR"
     gene            1569..2864
                     /locus_tag="VFF28_07535"
     CDS             1569..2864
                     /locus_tag="VFF28_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HZX45504.1"
                     /translation="MKNKTFVCFLLAGSLASAAAYGSELSSGDIAFVSIAAALVMLMT
                     PALGFFYAGLVRKKNLVSTLVQCIAIFAVISLVWALWGYSLAFGPSIGGFIGDLSLAG
                     LKGVGASAAMAYAPTIPALLFFFFQLKFAAITPALIIGAFAERINFKSLLIFIVVWTT
                     VIYVPIAHWVWNTGGWLRALGAIDFAGGTVVHISAGLSALAAALVIGRRKTNGNGSEF
                     KPNNIPFVVLGAALLWFGWFGFNAGSALAANALAVTALVLTNLSAAAAAISWMAVDWI
                     TKGKPSAVGLCVGAVCGLVAITPASGYVSVSASIIIGAVAGVLSNLVANWRASRTRLD
                     DSLDVFACHGVSGIWGSLATGLFASIAVNAAGANGLFYGNAHLLLSQATAVVVIGAYS
                     FFGSFIILKILGFFTNIRVSAEEEEAGLDYSQHGEEAYS"
     gene            2880..>3142
                     /locus_tag="VFF28_07540"
     CDS             2880..>3142
                     /locus_tag="VFF28_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HZX45505.1"
                     /translation="MDLIKETLEKAKKDAIKFVMLQFTDLHGVLKSVTIPAHKLPESL
                     EKGTWFDGSSIEGFTRIAESDMFLKPDPVTYALVPWEQGTARV"
BASE COUNT          791 a          622 c          723 g         1006 t
ORIGIN      
        1 tgccgcctga tcttgccagt gccatcatca agacccttga agacatggct gtagctggaa
       61 gagagatgtt gtatgttcca tctgctgcac tatatgtgtc tgtgccgcca aaccatccgc
      121 tcatgttata aggcattgcc atctctgaat aaaccatgtt tcctggctcg aaaagatagt
      181 ttatgatgtt taactcatcg aacgcgcctg tgccgtttgt aagattgaac ctgccgctca
      241 ccctgcttag ggtcatagat acattgaata cgatgttaac atgctttgga tcagtatagc
      301 ttgaaaggtt gcttacatca taatatatag gcatcgactg ctggggaaat atctctacag
      361 agtaattcct atctgagggg atatagattg tcttttgtgt tactgctgtt gtccactcag
      421 actcgatatc atatcccagt tttgtatctt ttaccatgta cttgaagctg acagcatctc
      481 ctgatgcatt tactgctgtg acgttaagcg tggctccttc cttgaggtag aacttgatgt
      541 ctgaaatatt gagcatagcg gccagttctt gatatgggag atgtggaaga ttagagccta
      601 catgcgttgc atctccagcg gcgctgaatt tcttcggtgt aaacttgatc atcttacctg
      661 caagatcttc gggggtaagg ttggttacaa ctttccaata tccagtggca ttgctaaggt
      721 ttgaatatgc ggcataaagt gttggaggtt ggccttcctg cattatatat actccaacag
      781 taacgtttgt attgttcagg gcatcttcag agacattgta tgcataacct gaaaaatcca
      841 ctgcgcttgc agaaaaagcg catgctaaaa ccacaagaca caaaagaacc aacctcttat
      901 ttatcatata aatcaccttt ttaatataca ttgttcacca tgtaagcctt atataaacgt
      961 ttctaaggat ttgtaaccac ttcaaagtca tctcaatccg atctcttttt cctttatcgt
     1021 atttagtata agccttgtat ttgtcctttc gacaaaatca taagtctgaa tcttttttag
     1081 gaaagaatcc atagccttcc tgttttgaaa ccttgcgagg atggccgcat cgaagtcacc
     1141 tgtgaggtca tatactgcaa agacattcgg atggtttgct atctcctttt ccacctggaa
     1201 gagttttcct tttgagatgc gcatctcgac aaggacttca acatcaaagc ctgccttgtc
     1261 atagtctatt atagtagtat atctctttat caccttctca ttttccagcc gctttaccct
     1321 atgcatggcg gtcgctacag aaacatttac ctcttttgcc agctcacgat aggatagttt
     1381 tgagtttaag agaagggcgt ttatgagctt cttgtctgtt tcgtcaaaag ccatgttaat
     1441 aggtgtttag tttaaggtta tttatatcgt atttattaaa catatattta aatatttctt
     1501 ttttttgtta gcatttgttt agtaaaatgg taaaaagatt tatgaatcaa ctaatgattg
     1561 gatgatagat gaaaaacaaa acctttgtat gctttctttt ggcaggaagc cttgcttctg
     1621 ctgctgccta tggctctgaa ctgtcttcag gagatatcgc ttttgtatct atcgctgctg
     1681 cacttgtgat gctgatgacg cctgcacttg gatttttcta tgcagggctt gtgagaaaga
     1741 aaaatcttgt gtctaccctt gtccaatgca tcgcgatatt tgcggtgata agccttgtat
     1801 gggctttatg gggatatagc ctggcgtttg gacctagcat cggaggtttt atcggagacc
     1861 tatcacttgc agggcttaag ggtgttggag cttctgcagc catggcttat gcacccacaa
     1921 ttcctgcgct cctgtttttt ttctttcagc tgaagtttgc tgctatcacg ccagcactta
     1981 tcatcggagc gtttgctgaa aggatcaatt ttaagtcttt gcttatattt atcgtcgtgt
     2041 ggacaactgt gatatatgta cctatagccc attgggtgtg gaatactggc ggatggctta
     2101 gggctcttgg agcaatcgac tttgccggcg gaacagtagt acatatctct gcaggacttt
     2161 ctgcacttgc cgctgctctt gtaatcggaa gaaggaagac taatggcaat ggctctgagt
     2221 ttaagcctaa caatatccca tttgtagtgc ttggggctgc tcttttatgg ttcggctggt
     2281 ttgggtttaa tgcaggaagc gctcttgctg caaatgcact tgcagtcact gctcttgtgc
     2341 tcacaaatct ttctgctgca gctgctgcga tcagctggat ggcagttgac tggatcacaa
     2401 aaggaaaacc atctgctgtt gggctttgtg tcggcgcagt gtgcggcctt gttgccatca
     2461 ctccggcatc tgggtatgtc agcgtgagtg catctattat aataggggct gtggcagggg
     2521 tattgtcaaa tcttgtggcc aactggagag cttccaggac aaggcttgat gactcacttg
     2581 atgtatttgc atgccatggt gtcagcggaa tatggggctc gcttgcgaca ggactttttg
     2641 cttctattgc tgtcaatgct gctggtgcta atgggctatt ttatggaaat gcgcatctgc
     2701 tattatcgca ggctactgca gtggttgtga tcggagctta ttcatttttt ggatcattta
     2761 ttatacttaa gatattgggg ttctttacca atatcagagt tagtgctgaa gaagaagagg
     2821 ctgggctaga ttacagccaa catggagaag aagcatatag ttaaaacaca ggtggtaaaa
     2881 tggatttgat aaaagaaaca ttggaaaagg caaaaaagga tgcaataaaa tttgtgatgc
     2941 tgcagtttac tgacctgcat ggagtgttaa aatcagtgac gattccagcg cataagctgc
     3001 cagaaagcct tgagaaagga acatggtttg atggaagctc tatcgaggga tttacaagga
     3061 tagcagagtc agacatgttt ctcaagcctg atcctgtgac atatgctttg gtgccatggg
     3121 aacaaggcac ggcaagggtt at
//
LOCUS       DASRUK010000174         4027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1811068, whole genome shotgun sequence.
ACCESSION   DASRUK010000174 DASRUK010000000
VERSION     DASRUK010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811068"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..430)
                     /locus_tag="VFF28_07545"
     CDS             complement(146..430)
                     /locus_tag="VFF28_07545"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmsC/YnfH family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HZX45506.1"
                     /translation="MMGLSRKPNMFESILLIIGVVLIVLGYYVIYKVYSIEGALNWNL
                     LQTLFLWILMILLIIVAAVNENMKEELKIIASNQLEEVKMLRKELTRRRF"
     gene            504..2060
                     /locus_tag="VFF28_07550"
     CDS             504..2060
                     /locus_tag="VFF28_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45507.1"
                     /translation="MEGQPYYQTQYLQGGRPVSRVSQGKRAIELVLLFCIVLANVVEF
                     LGFLPGDLDVLDKVLAWIALAYLFIDTSLTTILVGYRKYKVDVIIVIAYLMFIVKDFT
                     SFVQVGVEEVSLFRELFEFISKNISLFDRYSFYIGALLLVFISLYLALRAEVRQPSFM
                     AVLHEVGPPAQSIMQFMVRFISFLLVLVAFYIAIFNLAAEWLAVALDADLVIIGIFVY
                     FFIIVRYHKHLGPSNLLYKLGSLGEGFYQRFIQMFHYKQTLYLGITGLLALHLLADLG
                     HFIVPYILGFRDAMYFTQLGEGHTPLFTLFMADAAGKAASESIAILVVYLLNAAAMAF
                     LLILPAFVWYRFFKGRTVLVSRTSLALVFSSLMTFALSPAFMIKKISVEGLAGVDIVT
                     KSVVASPSLIDKFFPDKASGLIVLAIFSIFLGIVLWLLEYSQRIEKDVFIFAVVIGLV
                     YFGVYIFNYFVSIMSYYLSMVSLLMQHSEYLIASFLMLFALTSAVFYIGGYLFFIFEV
                     GKRHFFSSLR"
     gene            2110..2874
                     /locus_tag="VFF28_07555"
     CDS             2110..2874
                     /locus_tag="VFF28_07555"
                     /inference="COORDINATES: protein motif:HMM:NF017482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HZX45508.1"
                     /translation="MQLELDTKKSVEQNAAVYYDRAKKIKKKIEGAKTALAQSRLKLA
                     ELEKKEIKEIKKKEAPRAKEWYEKFRWFYTSEGLLVIGGRDATTNEIVVKKHTDKDDL
                     VFHTDMIGSPFFVIKNTGSIGEASIKEAADATVTFSRAWKLEMQSSSVFYCRPDQLTK
                     EAPSGEFVPKGGFITKGKLTYAENKVNLAICLFEGKVMAGPLSSVKSHCKTFVEIEQG
                     RDKASSVAKYIEKKIGGSPDEIIRVLPSGGFKVKKV"
     gene            complement(2951..3688)
                     /locus_tag="VFF28_07560"
     CDS             complement(2951..3688)
                     /locus_tag="VFF28_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HZX45509.1"
                     /translation="MAEEIPPDVLKNLKSVTAKRARTVIDHIIKHGHITTEELEKKYG
                     YAHPPRAARDVREQGIPLKTVRVKGSDGRTIAAYAFDDLSNIDQDKLEGRKVFSKQFK
                     EELLHNSDCKCYICMEKYDERYLQIDHRVPYEVEGDIDYENRDINDYMLVCGSCNRAK
                     SWSCEHCENWSGKKDITICKSCYWSFPENYSHLAGKQTRRLDLVWADEETKDFDALVK
                     ESKEQSKALPDYVKDVLKKHSKNKQGN"
     gene            complement(3690..>4027)
                     /locus_tag="VFF28_07565"
     CDS             complement(3690..>4027)
                     /locus_tag="VFF28_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009768086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HZX45510.1"
                     /translation="DPPYQGVCSNRDSRYHQGIDFYEFVSELEKLNKKEIKYLVSYDG
                     KTGDKIHGKLLPKHLNLTRVSINAGLSSQATLLGRTEITMESLYISEGLMRNLKNIPS
                     RQSELLVFA"
BASE COUNT         1128 a          818 c          790 g         1291 t
ORIGIN      
        1 ttggtaaagt gtcattgggc ctcacctctt ttttgaggct catcatttta ccaacagata
       61 atcacagaat tagccatcct ataagtgtta cgcattcatc cccaaccaca agtggtgggg
      121 atttctgcta agaaatttaa atcttttaga atctccttct tgtcagctct tttctaagca
      181 tcttaacctc ttcgagctga ttgcttgcta taatcttaag ctcttccttc atgttttcgt
      241 ttacagctgc aactattatc agtaggatca tcaaaatcca taagaacagc gtttgaagaa
      301 gattccaatt aagggctcct tcgattgaat ataccttata gattacataa tacccaagca
      361 ctataaggac gactcctatg ataagaagta tggactcgaa catgtttggc tttcttgaaa
      421 gtcccatcat tacaggtttt caccactaac tctatataaa tattatggaa aagaataaat
      481 acctctcttc ttagatatcg actatggaag gccagcctta ttatcaaacg cagtatttgc
      541 agggcggcag gcctgtaagc agggtttcgc agggcaaaag agccatcgag cttgtcttgc
      601 tcttctgtat agtacttgca aacgtcgtag agttcctggg atttcttcct ggcgaccttg
      661 atgtcttgga caaagtcctg gcatggatag cccttgccta tttatttatc gatacgagtc
      721 taactactat cttagtaggt tatcggaaat ataaggtaga tgtaataata gtaatcgcct
      781 atcttatgtt tattgtaaaa gactttacaa gctttgtcca ggtcggtgta gaagaggtca
      841 gcctctttcg tgagctattt gagtttattt caaaaaacat atcattgttt gaccgatatt
      901 cgttctatat aggcgccctc cttttggtat tcatttcgtt atacctcgct ctgcgcgctg
      961 aagtgcgcca gccaagcttc atggctgtcc tgcatgaggt tggtccgcct gcacaaagca
     1021 tcatgcagtt catggtcagg ttcatttcgt ttctgcttgt gcttgtagct ttctacatag
     1081 cgatttttaa ccttgctgcc gaatggctgg cagtggcgtt ggacgcagac ttagtcatca
     1141 taggcatctt tgtttatttt ttcatcattg taagatatca caagcaccta ggcccttcaa
     1201 acctgctcta caaactagga agtctaggag aaggctttta tcagagattt atccagatgt
     1261 tccattacaa gcagaccttg tatctgggaa taactggtct tcttgcactt catctccttg
     1321 cagatctagg ccatttcata gtcccatata tccttgggtt tagagatgcg atgtatttta
     1381 cccagcttgg agaaggccac actccccttt tcacgctttt tatggcagat gctgctggaa
     1441 aggctgctag cgagagcata gcgatacttg ttgtctatct gttgaacgct gcagccatgg
     1501 cctttctttt gatacttcct gcttttgtct ggtacaggtt tttcaaagga aggacagtcc
     1561 ttgtctcaag gacatctctt gcgttggttt tttcatcact gatgaccttt gcactaagcc
     1621 ctgctttcat gataaaaaag ataagtgttg aaggcctagc aggagtcgat attgtgacaa
     1681 aaagcgtggt ggcaagccct tctctcatcg ataaattttt ccctgataaa gcttcaggcc
     1741 ttattgtact agccattttt tccatatttc tgggtatagt cttgtggttg cttgaataca
     1801 gccaaaggat agaaaaagat gtattcatat ttgcagtggt tattggcctt gtttatttcg
     1861 gagtatatat cttcaattac tttgtcagca tcatgtccta ctacctaagc atggtttctt
     1921 tgcttatgca gcattctgag tatcttatcg cttctttcct gatgcttttt gctctgacat
     1981 cggcggtatt ttacatcggc ggatatctgt tctttatttt tgaagttggc aagcgccatt
     2041 tcttcagctc attacgttaa ggctgcagtc agcatcaatt ttttaaacta actcactttt
     2101 ttatagcgta tgcagcttga acttgacacc aaaaaatcag ttgaacaaaa tgcagcggtc
     2161 tactatgacc gggcaaagaa aataaagaaa aagatagagg gcgcaaaaac agctcttgcc
     2221 cagagcaggc ttaagcttgc agagcttgaa aagaaagaga tcaaggagat aaagaaaaaa
     2281 gaagctccaa gagcaaagga atggtatgag aaattccgct ggttttatac aagcgaaggc
     2341 ctgctggtaa taggcggcag ggatgctaca acaaacgaga tagtggtaaa aaagcacact
     2401 gacaaggatg atcttgtttt tcatacggat atgataggct cacccttttt tgtgataaaa
     2461 aacaccggga gtattggcga ggcttccata aaggaagcag cagatgcgac cgtgactttc
     2521 tcaagggctt ggaagcttga gatgcaaagc tcctctgtct tttattgcag gccagaccag
     2581 ctgacaaaag aggctccttc aggagagttt gtcccaaagg gaggatttat tacaaaggga
     2641 aagcttacct atgcggaaaa taaggtgaat cttgcaattt gcctttttga aggcaaggta
     2701 atggcaggcc cgctaagctc ggtaaaaagc cattgcaaga catttgttga gatagagcag
     2761 ggaagggaca aagcaagctc tgttgcaaag tatatagaga aaaagatagg cggctcacca
     2821 gacgagatta taagggtgct tccaagcggt ggattcaagg taaaaaaagt gtgaattgcc
     2881 tttaatgatt gttaaatcca ataattttta gtggcttaac ggtggaagga gggacatttc
     2941 taaactagaa ttaattacct tgtttattct ttgagtgctt ctttaaaaca tctttcacat
     3001 agtcaggcaa ggctttggat tgctcctttg attccttcac tagagcgtca aagtctttag
     3061 tctcctcatc agcccataca agatccaacc gtctagtttg tttgccagct aaatgcgaat
     3121 aattttctgg gaatgaccaa tagcaagatt tgcaaatagt aatatccttt ttaccactcc
     3181 aattttcaca gtgttcacaa gaccaagatt tcgcacgatt acaagaacca catacaagca
     3241 tatagtcatt aatatcccta ttttcgtaat caatatctcc ttcaacttca tagggcactc
     3301 tatgatctat ttgcaaatag cgttcatcat atttttccat gcaaatataa catttgcagt
     3361 ctgaattatg caacaattct tccttaaatt gtttagagaa aacctttcta ccctcaagtt
     3421 tgtcttggtc tatattactc aaatcatcaa aagcatacgc tgcaatagtt cgtccatctg
     3481 agcccttgac cctaactgtt tttaatggaa ttccctgttc tctaacatct cttgcagctc
     3541 ttggtggatg agcataccca tactttttct ctagctcctc tgtagtaata tggccatgtt
     3601 taataatatg gtcaattaca gttctagctc ttttagctgt cactgatttt aaatttttta
     3661 ggacatcagg agggatttct tctgccattt tatgcaaaaa ccaaaagttc agattgtctt
     3721 gaaggaatgt tcttaagatt cctcattaat ccctcagaga tataaagaga ttccatagtt
     3781 atctcagttc tccctaaaag cgtagcttga gaagataatc cggcatttat actaactcta
     3841 gtaagattta ggtgtttggg caacagcttt ccatgaattt tatctccagt tttcccatca
     3901 tagcttacta aatatttaat ctctttttta ttcaattttt caagctctga aacaaattca
     3961 taaaagtcta taccttgatg atatcgtgaa tctctattgg aacatacccc ttgataagga
     4021 ggatcca
//
LOCUS       DASRUK010000175         8242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2172833, whole genome shotgun sequence.
ACCESSION   DASRUK010000175 DASRUK010000000
VERSION     DASRUK010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 8242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8242
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2172833"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..413
                     /locus_tag="VFF28_07570"
     CDS             90..413
                     /locus_tag="VFF28_07570"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZX45511.1"
                     /translation="MFQAKGVVDKIGMVFVVMCLATTSISFLLGIFISPRAWCSFCPM
                     GTVQRWEGGRKYQLKFDKGLCVNCNLCNKICPMQLTVNNSEIKPDCIKCGKCVKVCPK
                     KALMF"
     gene            complement(410..727)
                     /locus_tag="VFF28_07575"
     CDS             complement(410..727)
                     /locus_tag="VFF28_07575"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZX45512.1"
                     /translation="MDKKEVLISQLEDFKERLSGKMEIDKLIFFGSTAAGKPKKWSDI
                     DLIIVSKAFEQKKSYMRAKGLRDLSGFNYPMDILCYTPSEFKKLSQRPSIVKDALKQG
                     IAI"
     gene            complement(706..1080)
                     /locus_tag="VFF28_07580"
     CDS             complement(706..1080)
                     /locus_tag="VFF28_07580"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HZX45513.1"
                     /translation="MDKEVSNWLRQAIYDLDTAKDNFNIKRYSYAIFLSEQSVEKALK
                     SLFLKEFGEIPRVHDLTVLAKKLKVPDKLYGHCEDLTNVYMETRYPDVSEVIPAEKFT
                     RDEANSFISKAEEVLEWIKKKF"
     gene            1378..2448
                     /locus_tag="VFF28_07585"
     CDS             1378..2448
                     /locus_tag="VFF28_07585"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZX45514.1"
                     /translation="MGLFDNMLNSGESLIRNEVALDFSFQPKPMKYREAQQRQMASCI
                     QPLFQKRNGRNLFIYGIPGIGKTLANKQLLDELEEKTDEILVFYINCWNKNTSFKVYL
                     ELCEILGYRFTQNKAGEDLFKIIKEMINKKSAVFVFDEIDKAEDFDFLYSILEGIYRK
                     TIFIITNYKEWLTHVEERIRSRLMIESLEFKAYNKAETEGILRQRIEYAFAPGVLADD
                     AFSLILSKTVSLGDIRAGLTLLREAGLYAENRSSRKVELSDAQKALAKIDDYNMESSD
                     KLADDSKFILGIIKQNSEARMGDLFKAYSEKGGKLAYRSFFRHVKKLADDKFVSLDKI
                     EGGAEGTTTIVKYVKVKQLSEF"
     gene            complement(2445..2720)
                     /locus_tag="VFF28_07590"
     CDS             complement(2445..2720)
                     /locus_tag="VFF28_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018031853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HZX45515.1"
                     /translation="MERVHLIITGMVQGVWFRHNTNIIGNKLGLNGFVRNTPEGAVEV
                     IAEGDPTQLAKLIEFCRKGPASARVEDVKVDYEEAKNEFKGFEIRYD"
     gene            complement(3249..3554)
                     /locus_tag="VFF28_07595"
     CDS             complement(3249..3554)
                     /locus_tag="VFF28_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotyrosine protein phosphatase"
                     /protein_id="HZX45516.1"
                     /translation="MKILFVCNQNKNRSKTASDLFKGTFQTRSAGLYNGKPLTEKELS
                     WADTVIVMEDMHRSEIAKRFPDLYIQKRILSLDIPDVYHYNQPELVDILKVKIKELF"
     gene            3607..3732
                     /locus_tag="VFF28_07600"
     CDS             3607..3732
                     /locus_tag="VFF28_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45517.1"
                     /translation="MEEEEYDIYTQEGVGTYYEDDEIDGKEEGFMLGYLDAIRVD"
     gene            3782..4348
                     /locus_tag="VFF28_07605"
     CDS             3782..4348
                     /locus_tag="VFF28_07605"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZX45518.1"
                     /translation="MKILTEDEVKTNKEVILEVIRNGTIFIHPTDTIYGLGCNAESED
                     CVDDVRDIKERADSPFSVIAPSKEWILENCIVNGEGKEWIDKLPGPYTLILRSKKGVA
                     KNVAPGHDTLGIRMPDHWFSEIVRELGIPVVTTSANKSGENFMTSLDNLDDDIKESIE
                     FIIYEGEKHGHPSTIVDLTGEKAIVRKR"
     gene            4580..4849
                     /locus_tag="VFF28_07610"
     CDS             4580..4849
                     /locus_tag="VFF28_07610"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HZX45519.1"
                     /translation="MEQKNLISEFAIASVVIGILSFFQLFGLEKPIAAIVFGILAIRS
                     IRKNKLRGRNLALAGIILGVAAIITMIIITVIYYPQLQQMIQPTG"
     gene            4851..5627
                     /locus_tag="VFF28_07615"
     CDS             4851..5627
                     /locus_tag="VFF28_07615"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZX45520.1"
                     /translation="MDKTEGKKDKLKILAAGDNENSGFSSLQQPKTSKLKILAAGDNE
                     NSGFSSLQPHKSDKLKILAAGDIHGDIGLAEKLAEKALKEKVDLVVLCGDLTLGERST
                     DNIIGPFKKRNEKVLLIPGNHETVATADFLAELYGMKNIHGYSVKYKDVGIFGAGGAN
                     IGVFQMEEKEIYDLLKKGHDKIKDLKKKIMVTHVHPTGTKMEKFTDIFPGSEGVRRAI
                     EKFKPALLLCSHVHEAEGIEEMIGKTKVVNVGKRGKIIEI"
     gene            5638..6189
                     /locus_tag="VFF28_07620"
     CDS             5638..6189
                     /locus_tag="VFF28_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HZX45521.1"
                     /translation="MNTLLIVGIIVVVLVLFVILIYNSLIRLKNQVKNAWAQIDVQLK
                     RRTDLIPNLVETVKGYMKHEKEVLENVTRARSAVMKAGSVHEKAAASNMLSDTLKSLF
                     AVAENYPTLKANENFLQLQEELSGTENKIAYSRQHYNDIVMHFNNKIQTFPNNIFANM
                     LNFKQEESFTATEAERKNVKVKF"
     gene            6196..7092
                     /locus_tag="VFF28_07625"
     CDS             6196..7092
                     /locus_tag="VFF28_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HZX45522.1"
                     /translation="MRLAFEEIRGNKIRSFILIFLFILIIGMLGAVIGLIVNNLYFGL
                     IIAMVFSIFYSLIGYYSGDSMILTMSGARPVTKQEFPYLYHTLEGLAIAAGIPTPKAY
                     VIEDSAINAFATGRDPKHASITVTTGLLKVMNRQELEGVIGHEMSHISNLDIRFMMLT
                     TVLVGIVVLLSDFILRSFLWGGKGRNRNESKAGAALILVGLALAILTPIIGEIMKLAI
                     SRKREYMADANAAVLTRYPPGLASALRKIAKDPDPLVDMANKSTAHLFISTPFRKERS
                     FVTNLFSTHPPLPERIKRLEEM"
     gene            7093..7971
                     /locus_tag="VFF28_07630"
     CDS             7093..7971
                     /locus_tag="VFF28_07630"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZX45523.1"
                     /translation="MKKRTFYLVLAIVVLIIIAVIAYRAINQKETYTFKVDKFSYTHR
                     GKAKYTETFLNKTGNISVYSVTFPSRNFLTYPITIYGLLFIPTGKKGGVVLLPGGGVS
                     KEAESRLANILAEKGYAVLTIDQRGIGETGGYYLGFDDDYRVFLKGDEPIQHLSVYDA
                     LRAYDVLKEIYGKDEIAMMGESMGGRYAMIAGAVEKRLKGVLVISSSGFHFDNSNPYL
                     LSIDPDHYVSDIAPRPLVMLHGDNDSTVKIDDAKITYSLARQPKSFFIAPGCRHGYCG
                     KMKETMLDGLKEILGE"
BASE COUNT         2529 a         1493 c         1838 g         2382 t
ORIGIN      
        1 aatttggttc ttaatgccat ctatatcacc tcttgctaat tacagaaggg gtacttatat
       61 aagaactttt ctatttaact tactatagat tgtttcaggc aaagggagtt gtcgataaga
      121 tagggatggt ctttgtagtg atgtgtcttg cgacaacttc tatttctttt cttttaggga
      181 tatttatcag tccaagggca tggtgctctt tttgcccgat gggaacagtg cagagatggg
      241 aaggcgggag gaagtatcaa ttgaagtttg ataaaggcct ttgtgttaac tgcaacctat
      301 gcaataagat ctgcccgatg cagctgactg tcaataacag cgagataaag cctgactgca
      361 taaaatgtgg aaaatgtgtc aaggtatgcc ctaagaaggc gctgatgttt taaatagcaa
      421 tcccttgttt tagtgcatcc tttacgatag atggtctttg tgaaagcttt ttaaattctg
      481 agggtgtata gcataggatg tccataggat agttaaatcc agacaggtct cttaatccct
      541 ttgccctcat ataactcttt ttttgctcaa atgctttgct tacaataatc aagtctatgt
      601 cgctccattt ttttggcttt ccagcagccg tcgaaccgaa aaatatcagt ttatctatct
      661 ccatcttgcc actaagccgc tccttaaaat cctctaattg agatattaaa acttcttttt
      721 tatccattcc aatacctcct cggctttgct gataaatgag ttggcttcat ctcttgtaaa
      781 cttttcggct gggataactt cgcttacatc gggatatctg gtttccatat agacattggt
      841 caggtcttca caatgcccat ataacttatc tggaacctta agcttttttg caagaactgt
      901 cagatcatgc actcgaggaa tctccccaaa ctccttaaga aaaagtgatt ttagcgcttt
      961 ttcgacagat tgttcgctta ggaagatagc ataactataa cgctttatat tgaagttatc
     1021 cttggcagta tccaaatcgt agatagcttg tctaagccaa ttactcactt ctttatccat
     1081 atagtgaatt ctaatactgg ctgatatata aagtttgagg gatgcatttg atacatagaa
     1141 aaagagacat taatctaaca aggcagttta taaatcgttt tggctgtcaa ttcccgacaa
     1201 aacctaaaaa tctgtcagac aaatcatcgt tttgctaatc taattgacgg tagattaata
     1261 tattttagag cattcttgtg aaatacgact agggggttat tcatctttta ggcagaagtg
     1321 cctttttttc ttttttttgc cataagcttt aaaaactttt aatcattacc tcaaagcatg
     1381 ggcttattcg acaacatgct taattctggc gaatctttga taagaaacga ggtggctctt
     1441 gatttctctt tccagccaaa gcctatgaaa tacagagaag cccagcagag gcagatggct
     1501 tcctgcatac agcctctttt ccagaaaaga aacggaagaa acctgtttat ctatgggata
     1561 cctggaatag gaaagacgct tgcaaacaag cagcttcttg acgagcttga ggaaaagacc
     1621 gatgagatac ttgtcttcta tatcaactgc tggaataaaa atacttcttt taaagtttat
     1681 cttgagcttt gcgagatact gggttacagg tttacgcaga acaaggcagg agaagatctc
     1741 tttaagataa tcaaagagat gataaacaag aaatcagctg tctttgtgtt tgatgagata
     1801 gacaaggcag aggattttga ttttctttat tctatacttg agggaatcta taggaagacg
     1861 atatttatta taaccaacta taaagaatgg cttacccatg ttgaggaaag gataaggtca
     1921 aggctgatga tagagagtct cgaattcaag gcttacaata aggctgagac tgaaggaata
     1981 cttaggcagc gcatagagta tgcttttgcc ccgggtgttc ttgctgatga tgcattttct
     2041 cttatactat caaaaacagt atctttagga gatatacgag caggacttac cttgcttagg
     2101 gaagcagggc tttatgctga aaacaggtcc agcaggaaag tagagctttc agatgcacag
     2161 aaagcgcttg caaagataga tgactacaac atggagagct ctgacaagct tgctgacgat
     2221 tcaaagttca tcttggggat aatcaagcag aacagtgaag cgcggatggg agacttgttc
     2281 aaggcttact ctgagaaagg aggaaagctt gcctaccgca gcttttttag gcatgtgaaa
     2341 aagcttgctg acgacaagtt tgtaagcctt gataagatag agggcggtgc tgaagggact
     2401 actactatcg ttaaatatgt aaaggttaaa caattgagtg agttttaatc ataccttatc
     2461 tcaaagcctt tgaactcatt ctttgcctct tcatagtcga ccttaacgtc ctctactctt
     2521 gctgaggctg ggcctttgcg gcaaaattcg ataagctttg caagttgagt agggtcacct
     2581 tctgctatga cctctacagc accttctgga gtgttcctga cgaatccgtt caagcccagt
     2641 ttgtttccta ttatgtttgt attatggcga aaccaaactc cctggaccat gcctgttatg
     2701 ataagatgaa ctctttccat tgcagggatg gtaaggattg aaaatattta aggttatttc
     2761 ttttggcaag tggatatgcg ccttaacttc tgtaaatttg gcctttatag cctatgctaa
     2821 tgagcacttg ccacttgcca aagcctgacg ccggctatat aaatgggctt tagcataata
     2881 tctctattag cacttaaaaa ttaaggcagg aaaaagtaac acaccccctt gtgtcctcct
     2941 gccttatttt tttaataaag aggtgaagaa caatggtaga tttaagaaag gaaacgatac
     3001 aagagctttt ttacagccaa atcagaaaga taacgatgga caaggcacgc agctttgaca
     3061 tccagttttc agatgcgtgg atatagaacg aatgaataat ggcaagcgaa tgagagatat
     3121 ccatccttca gttcgaggat aatgaaggga taaagtacaa agtaactcgg agctacccag
     3181 acttgtcaat tgcagaaact aaggtattta atctccttga agatgctaag aagcaatttg
     3241 acgattggtt aaaataattc ctttatttta actttgagaa tgtcgacaag ttcaggttga
     3301 ttataatggt aaacatcagg aatatcaagc gagagaatcc ttttttggat gtagaggtca
     3361 ggaaatctct ttgctatctc actcctatgc atgtcttcca taacaataac tgtatcggcc
     3421 cacgaaagct ctttttcagt aaggggcttt ccattataaa ggccggcgga tctagtctga
     3481 aaagtacctt tgaacagatc actagctgtc ttgctccgat ttttattttg gttgcagaca
     3541 aataaaatct tcacattaat cagtagaata cttgtagata taaagtttgt taaagaggtg
     3601 atacccatgg aagaggaaga atatgatata tatacccagg aaggggtagg gacatattac
     3661 gaggacgacg agatagacgg caaggaagaa ggattcatgc tcggatacct tgacgctatc
     3721 agagttgact aagagtcttt ttccgcaaat tatataaata tattttagtt tgctcttttt
     3781 gatgaaaatc cttacagagg atgaagtaaa gaccaataaa gaggttattc ttgaagttat
     3841 taggaacggt acaatcttta tacacccgac agatactatc tatggtcttg gctgcaatgc
     3901 agagtcagaa gattgcgtcg atgatgtccg cgatattaag gaaagggctg attcaccttt
     3961 ttctgtcata gcgccctcaa aggaatggat acttgagaat tgcatcgtca atggggaagg
     4021 aaaggaatgg atagacaaac tgcctgggcc ttatacactt atccttaggt caaaaaaagg
     4081 tgttgctaag aacgtggcac ctgggcatga cacacttgga ataaggatgc ctgaccattg
     4141 gtttagcgaa attgtgaggg agctagggat tccagtggtt acaacctctg caaacaaatc
     4201 aggggaaaac ttcatgacat cgcttgacaa ccttgatgat gatatcaaag agagcataga
     4261 gtttatcatc tatgaagggg aaaaacatgg gcatccttct acaattgtag acctcaccgg
     4321 ggaaaaggct attgtcagaa aacgatgaat aagtttttaa acaatgaaat tccaccattc
     4381 tattaggatg gaatgagttg acttagaaac gattgtttct aagttcctag agatcctatg
     4441 gatttctatg atgttcaaaa accacagaag atggtggagt ttcaagcgtc aaaaaccact
     4501 gtccgctttc agtgcataga aacatgccat cggtccgtaa gctgtggttc ttgacataag
     4561 ctttctatct gaggtaccta tggaacaaaa gaatcttatc agtgaatttg ctatcgcaag
     4621 cgttgtcatc ggaatacttt cttttttcca gctttttggt cttgagaagc cgattgctgc
     4681 tatagttttt ggaatacttg caataaggag catacggaag aacaagctaa ggggaagaaa
     4741 ccttgcctta gcaggaataa tcttaggggt cgctgcaata ataacgatga taataataac
     4801 tgtgatctat tatcctcagc tgcagcagat gatacagccg actggttaat atggacaaaa
     4861 ccgaaggaaa aaaggataaa ttgaagattt tggctgccgg agacaatgaa aactccgggt
     4921 tttcaagcct ccagcagcct aaaactagta agctcaagat actggctgcc ggagacaatg
     4981 aaaactccgg gttttcaagc ctccagccgc ataaaagtga taaattgaag atacttgcgg
     5041 ctggagacat acatggagat atcggccttg ctgagaagct tgctgagaag gcactgaaag
     5101 agaaagtcga ccttgtggtt ctttgcggcg atcttacact tggggaaagg tctactgaca
     5161 acataatcgg gccttttaag aaaagaaacg agaaggtttt gcttatccct ggaaaccatg
     5221 agactgttgc tactgctgat tttcttgctg agctctatgg gatgaaaaat atccatgggt
     5281 attctgtgaa atacaaggat gtgggcatat ttggcgccgg cggggcaaac atcggtgtct
     5341 ttcagatgga agaaaaagag atatatgacc tgctcaaaaa agggcatgat aagataaagg
     5401 acctaaagaa gaagataatg gtgacgcatg tgcatccaac aggcacaaag atggaaaagt
     5461 tcactgacat attcccaggc tctgaaggtg ttaggagagc tatcgaaaaa ttcaagccgg
     5521 cgcttctctt gtgctctcat gtacacgaag cagagggcat agaagagatg ataggaaaga
     5581 caaaggtagt aaatgtcggc aagaggggaa agataatcga gatataggag ggagaatatg
     5641 aacacattgc ttatagttgg aattattgtc gttgtgctgg tgctgtttgt gatactgatt
     5701 tacaacagcc tgataaggct aaaaaaccag gtaaagaatg cctgggcgca gatagatgtg
     5761 cagcttaagc gaaggactga tctcatccct aatctggtag agactgtcaa gggatatatg
     5821 aagcatgaga aggaagtgct tgagaacgtg acaagggcaa ggtctgctgt gatgaaggca
     5881 ggcagtgtgc atgagaaagc ggctgcttca aacatgctaa gcgatacctt gaagtcactt
     5941 tttgcagtcg ctgagaacta tcctacgctt aaagccaatg aaaacttttt gcagctgcag
     6001 gaagagcttt cgggtacaga gaataagata gcatactcaa ggcagcatta taacgatata
     6061 gtcatgcatt ttaacaacaa gatacagact tttcccaaca acatctttgc aaatatgctt
     6121 aacttcaagc aagaggaaag ctttactgca acagaggcag agagaaagaa cgtaaaggtg
     6181 aagttttagg actttatgcg gcttgctttt gaggagataa ggggcaacaa gataaggtct
     6241 tttatcctta tctttctttt tatattgata atcggcatgc ttggagctgt tatcggactt
     6301 attgtaaaca atctttattt tgggctgata atcgctatgg tcttctctat cttttattcc
     6361 ctgataggat attattcagg agattctatg atcctcacca tgagtggagc aaggccagtc
     6421 actaaacaag aatttcctta cctttatcat acccttgagg ggcttgcgat agctgctggc
     6481 attccaacac ctaaagctta tgttatcgaa gattctgcga taaatgcttt tgccacagga
     6541 agagacccca aacatgcgtc tatcactgtg actaccggac tgcttaaagt catgaacagg
     6601 caggagctgg aaggagttat cgggcacgaa atgtcacata tctctaactt ggatatcagg
     6661 tttatgatgc ttaccactgt ccttgttgga atagtggtac tgctaagtga ttttatactc
     6721 agaagctttc tttggggagg aaaaggcagg aatcggaatg aaagcaaagc tggtgctgcc
     6781 ttgatattgg tagggcttgc gcttgctatc cttactccga taatcggaga gataatgaag
     6841 cttgccataa gcaggaaaag agaatacatg gctgatgcaa atgctgctgt attgacaaga
     6901 tatcctccag gacttgcaag cgcacttcgc aagattgcca aagatcctga tcctcttgta
     6961 gatatggcaa ataaatcaac tgctcatcta ttcatatcga caccttttag gaaagagaga
     7021 agttttgtca ctaacctgtt ctctacgcat ccgcctttac ctgaaaggat aaagagactt
     7081 gaagagatgt agatgaagaa gagaactttt tatcttgttt tggctattgt tgtgctaatc
     7141 attattgcag tgatagcata ccgagctatc aaccaaaaag agacttatac atttaaggtt
     7201 gacaagtttt catatactca tagaggtaag gccaaataca ctgaaacatt tctgaataaa
     7261 acaggcaata tcagtgttta ttctgtaact tttccaagca gaaattttct tacttatcca
     7321 ataactatct atgggctttt gtttatacct actgggaaaa aaggaggggt tgttctattg
     7381 cctggaggag gagtttcaaa agaagcagaa tcaaggcttg caaatatact tgcagaaaag
     7441 ggctatgcag tgctaacaat cgaccaaaga ggaatcggtg agactggagg atattacttg
     7501 ggttttgacg atgattatcg ggtctttttg aagggcgatg agcctataca gcatctgagc
     7561 gtctatgatg cgttaagggc ttatgatgtg ttgaaggaaa tctatgggaa agatgagatt
     7621 gccatgatgg gagaaagcat gggtggaaga tatgcgatga ttgcaggagc tgttgaaaag
     7681 agactgaaag gagttctggt gataagcagc tctggctttc attttgataa ttccaacccc
     7741 tatctcttat caatcgatcc cgaccattat gtttcggata ttgcacctag accacttgtc
     7801 atgctgcatg gagataatga cagcactgtc aaaattgatg atgccaagat aacttattct
     7861 cttgcaaggc agcctaagag cttctttatt gcccctggtt gcaggcacgg gtattgcggt
     7921 aagatgaaag aaacgatgct tgatgggttg aaagagatac ttggcgagtg ataggttttt
     7981 aaagaacttc gggttcttaa ggggaatgga taaatataga gctcctatcg ctatacggaa
     8041 gggtaatcat acttatatat ctgtaacttc acatgaagca caatctctat atttgctatt
     8101 gcaacctgac ctcgccactt caacagtttt ctatctaatt ttaacactaa ctcagtttga
     8161 cagttgggat tttaccagcg cataggcagg ctcttgtccc gatatttctc tataaaatca
     8221 cccaaaatgc caactatttc cg
//
LOCUS       DASRUK010000176         8312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1985494, whole genome shotgun sequence.
ACCESSION   DASRUK010000176 DASRUK010000000
VERSION     DASRUK010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 8312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8312
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1985494"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1575)
                     /gene="feoB"
                     /locus_tag="VFF28_07635"
     CDS             complement(<1..1575)
                     /gene="feoB"
                     /locus_tag="VFF28_07635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HZX45524.1"
                     /translation="MRGCHSCKDDKLVTADIALVGNPNTGKSCLFTQLTGINTIISNY
                     AGTTVDIQSGKFSFKGKTYVLVDLPGTYSLGSATDEQKAATGYIFNKKPKVIVNVVDS
                     TLLERNLYLTLQLLELKIPIVIALNFCEECADKGKSIDPEKLSSILGVPVVSIDALRG
                     AGIKELMDKAMEAYDGNLPFKYYSMKYDDHIEKAIDKISPLIRREWIPKRASAVGLIE
                     EKDEGSDEYKKVKKVIDSLSTEHALGTEISRERHGQAALIAEKVIKLGKVKKSFKEYL
                     DRFTTERATGILSMFVVLAIIFSSLFFLGGWLSTLFGALFEKFILGPLNPLIEKIPSE
                     LAVQMIKWGLDGINAALQIALPYIAVFYILLALLEDSGYLPRMAYILDRITHKLHLHG
                     KAIVPMMLGFGCSVPAILSTRILPSRKERILTSILISLVPCSARTAVILGSVGAFIGP
                     MYALLIYLIILLLIFTVGFVLGRLLPGESQGLILEMPEYRIPYLKNVFIKTWLRVKDF
                     VTIAFPLIIIGSMALGV"
     gene            complement(1572..2198)
                     /locus_tag="VFF28_07640"
     CDS             complement(1572..2198)
                     /locus_tag="VFF28_07640"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HZX45525.1"
                     /translation="MSISKEDYLGEIYRVQQKNNRAAKLTEIAASLKVSKPSTSQMVR
                     KLSKEGLVEFERYSGITLTKKGIEEARTLIRKHQLLEVFFNKFLNIKHRFHHEAHNME
                     HGLSNEAADRLDALLKNPALCPDGNPIPRENIMELRELSIGGEADILFCTTQDKKCME
                     QLNSLGLVPRTRVKVIRKIKNGPLMLKVKDSEIALGNDVCSKVYVERI"
     gene            2305..3228
                     /locus_tag="VFF28_07645"
     CDS             2305..3228
                     /locus_tag="VFF28_07645"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZX45526.1"
                     /translation="MDETMKKHVIHMIIMFTAVITLLLLGSYNISKLRQDYNGKIAEL
                     NYGLQDKQGQIDDLSNKLAATNQLLAEANEKIGIKLDDIEATSKKYGSQIEKIQETSE
                     QYEMQLGELNQQLSDLAVDSESFSSIISGVINGVVSVVTNKGQGSGAIISSDGYVVTN
                     YHVIAGARSASVMTYDGRTHRISLEGYDETQDLAVLKIMTNETFERLLFGDSSNLKAG
                     QKVVALGNPAGLSFTATEGIISSPERTLDDGLKYIQTDVTLNPGNSGGPLINSQGKII
                     GIVNSKISGYEELGFAIPSSRVKDVVDEIVG"
     gene            3384..4019
                     /locus_tag="VFF28_07650"
     CDS             3384..4019
                     /locus_tag="VFF28_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45527.1"
                     /translation="MKKKYFIYFAILAVLAAVAFGGRSPAVEDFAFSCVYGGEQCLPV
                     VDTTVGICRTLQFATPDGLIGVLLCHKDDLRYELYRQFYPGAKDFKVCTADDAGCIDS
                     LRGFDSFTTHEFDFEPSPTDYCYKPIENFQVTCGAPGCHVTTDTTIGTCRSVVFASDL
                     GPGQILACHKGDNRYEVYSQQGAYKRCLGPGCVEGAQGFDSFEITCPPLAG"
     gene            complement(4110..6116)
                     /locus_tag="VFF28_07655"
     CDS             complement(4110..6116)
                     /locus_tag="VFF28_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZX45528.1"
                     /translation="MQQRKEKRQHFRKRIKNLIIGKARNIQDPLLFHKISLIAIFAWI
                     GLGSDALSSSAYGPAEAFLALGKYSHLSVFIALMVASTILIISASYSQIVKLFPQGGG
                     GYIVASKLLSPTAGMISGCALLIDYVLTITVSIASGVDAIFSFLPVEYHQWKLMFALL
                     AIVFLIVINLRGVRESIFSLLPIFALFVLTHIFALAYGLFTHAPQVSQTITATAGDVS
                     SALSSLGLFGFLFLLLHAYSMGAGTYTGIEAVSNAMPVLREPKVLTARRAMFYMTWSL
                     VILVLGLMISYTLFGVEHVPGKTLNAVLFENIASSWPPLLGRAFVLITLISEAALLFV
                     AAQTGFLGGPSILANMAGDGWLPKRFTVLSDRFVIQNGILIMGFASLFMMILTRGSIG
                     FLILLYSISVFITFCLSQLGMVHYWWANKIAGWIKGIFLNGLGLLISVLILGSMLIVK
                     FKSGGWITLFLLLILILVTMRIKQHYTKSKIRLQKLDYLIDVAESSKPQPEIPIVYNE
                     DRSDEYVHPYEKTAVVLVNGFNGMSLKTLANIFQSFGGLFKNFVFVRIGVIEAGLMKS
                     QQELNQLDCDIKCESQKYIQFMNKYGYYAESITIVGTDVVSEVIKIAPEILARFPNSV
                     FFGGQQVFSEVRTMTKVLHNYTVFAIQEQLFKRGATCIILPLNV"
     gene            6358..8037
                     /locus_tag="VFF28_07660"
     CDS             6358..8037
                     /locus_tag="VFF28_07660"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HZX45529.1"
                     /translation="MAFLFKKKVKGIYSLYMGENKKVNGISTRVKSKYLGPLHRFKEY
                     FADDLFKIERINHYEYGLSSALKQIMDQFLFQKIFRNKIKKQTEDPYIDRLMVLIVMN
                     RLADPCAKYSLQRWFKGTDLANTFNIPVEELEVHKIYRAMDQLDKYAEEIETEICKTI
                     LIQENISLNMIYLDFTNQESYSRNHDSELLKNGHNKRGHDELLQVNLSLTCDSNTGIP
                     FFHKSYPGNYNDKQFIKIYAEELRAKLDALGYTGKNTLIIDRGINGDKDFELLRQHRF
                     DYIGGLIQKEFPKYFEIPKSKLRNTHIHTRETKEDVKIKYTGIKEEIYGQNHLILTLY
                     NVESYDSAIEDLDNSLKEYKVACEKQLQEFKKEIAEKTFQSHWNNVDKITNKLKEINK
                     EIYPLLRFKIKSYRFDLTWKINQNKKEYNKYVDNFGKYVLFTNRLDLKPKEVIELFYG
                     KKKIEKNFHYLKSNGYTNRYLRMGPMLHSKDERIKSHNYTCVSALQIYQIINYRLKKK
                     GLDISAQEALDELKTITYYHIKVPGQDEPIRHVNLLTQRQKEILKALEVEL"
BASE COUNT         2554 a         1723 c         1759 g         2276 t
ORIGIN      
        1 cacaccaaga gccatactgc cgatgattat caagggaaaa gctattgtga caaaatcctt
       61 taccctaagc catgtcttta tgaatacatt cttaagataa ggaatcctat attcaggcat
      121 ctcaagtatg aggccttgag attctcctgg gagaagcctt ccaagcacaa agcctactgt
      181 aaatatcagt aaaaggatta tcagatatat caacaaagca tacatagggc caataaacgc
      241 tccgacagac ccaaggataa ccgcagtcct tgctgagcaa gggacaagtg atatcaagat
      301 agaagttaat atcctttctt tcctgcttgg aagtatcctt gtagaaagta ttgcagggac
      361 tgaacatcca aagccaagca tcattggaac gatagccttg ccgtgaagat gaagcttatg
      421 cgtaatcctg tcaaggatat aggccatcct tggaagatag cctgagtctt caaggagtgc
      481 aagcaggata tagaagactg cgatataggg aagtgctatc tgtaaggcag catttatccc
      541 atccagcccc cattttatca tctgaactgc aagttcagag ggtatctttt cgataagtgg
      601 atttagtggg ccaaggataa acttctcaaa gagtgctcca aacaaggtgc ttaaccagcc
      661 gccaagaaag aataaggagg aaaatattat cgccagcaca acaaacatcg acaagatccc
      721 tgtggccctt tctgtggtaa acctatcaag gtattcctta aaagacttct tgacttttcc
      781 aagctttatc actttttcgg caataagcgc tgcctggcca tgccgctccc tggatatttc
      841 ggttccaagg gcatgctctg tgctcaaaga atcgatgacc ttcttgactt ttttgtattc
      901 atcagagcct tcatcttttt cctcgattaa gcctactgcg cttgccctct tgggtatcca
      961 ttctctcctg atcaagggag atatcttgtc tatagccttc tctatgtggt catcatactt
     1021 catgctgtag tatttgaacg gcaaatttcc atcataagct tccatcgcct tatccatcag
     1081 ctcttttatc cctgctcctc gaagggcatc tatgctcaca acaggtactc caagtatgga
     1141 tgagagcttt tcaggatcta tggatttgcc tttgtcagca cattcctcgc agaaattgag
     1201 cgctatcact ataggtatct taagttccaa gagctgcaaa gtaagatata ggtttctttc
     1261 aagcagtgtg gagtccacga catttactat tacttttggt ttcttattga atatatagcc
     1321 tgtcgctgcc ttttgctcat cagtcgcaga gccaagtgag taagtgccgg gcaaatccac
     1381 aagaacatac gtctttccct taaaagaaaa tttgccagat tggatatcta cagtagtgcc
     1441 ggcatagttt gagatgatag tatttatccc ggtcagctgt gtaaacaggc agctttttcc
     1501 tgtgttggga ttgccgacca gcgctatgtc tgctgtaaca agcttatcat ccttgcacga
     1561 atggcagccc ctcatattct ctccacatag acttttgagc aaacatcatt tcccaaggca
     1621 atctcgctat cctttacttt aagcatcaat ggcccattct ttatcttcct gataactttt
     1681 actcttgtcc ttggcacaag cccaagggaa ttcagctgct ccatgcactt cttatcctgg
     1741 gtcgtgcaaa ataatatatc cgcctcgcct cctatagaaa gttctctaag ttccatgata
     1801 ttctctctgg gaataggatt tccatcaggg cacagagcag gattcttaag tagagcatcc
     1861 aacctgtcag ctgcctcgtt tgaaaggcca tgctccatat tatgagcttc atggtggaac
     1921 ctgtgcttga tattaaggaa tttattaaaa aagacctcaa ggagctgatg ctttcggatt
     1981 agtgtccggg cttcctctat ccccttcttt gtaagggtga tgccgctgta cctctcaaat
     2041 tcgactagcc cttcctttga caatttcctt accatctgtg aggtgcttgg ctttgatacc
     2101 ttaagagaag cagctatctc tgtgagtttt gcagctctgt tgttcttttg ctgcactcta
     2161 taaatttccc caagataatc ttctttgctt atgctcatca atgttagttt aacctaactt
     2221 atttataaat attatggagg acggattatg ccgctgacta atctttaata aagattataa
     2281 agccggccag attggcttga gggtatggat gaaacgatga agaagcatgt tatccacatg
     2341 ataatcatgt ttacagcagt cataaccctt ctccttttag gcagttataa catctccaaa
     2401 ttaaggcaag attacaatgg gaagattgca gagctaaatt atggcctgca agacaagcaa
     2461 ggacagatag acgacctatc aaacaagctt gcagcgacaa accagctgct tgcagaagca
     2521 aacgaaaaga taggtataaa gctggatgat attgaggcca caagcaagaa atatggatca
     2581 cagatagaaa agatacagga aacctctgag cagtatgaga tgcagcttgg cgaactcaac
     2641 cagcagctta gcgaccttgc agtcgatagc gagagtttca gtagcataat ctctggtgtc
     2701 atcaatggag ttgtaagtgt ggtgacaaac aaaggacagg gtagtggtgc gataatctcc
     2761 tctgacggct atgttgttac caactatcat gtgattgcag gtgcaagaag tgcaagcgtg
     2821 atgacctatg atggaaggac gcaccggatc agccttgaag gttatgatga aacccaggac
     2881 ttggcggtgc ttaagataat gacaaacgaa acttttgagc gtctcttgtt tggagattca
     2941 agtaacctca aagcaggcca aaaagtggtg gcgcttggaa atcctgcagg tctttctttc
     3001 acagcaacag aaggcatcat cagctcgcca gagagaacat tggatgatgg cctcaaatat
     3061 atccagacag atgtgacact aaatcccgga aactccggag gccctctgat aaatagccag
     3121 ggaaagataa taggaatagt taattctaaa atttcaggct acgaggagct tggatttgcg
     3181 attccaagca gcagggtaaa agacgtggtt gatgagatag ttggctaagg cggctagcct
     3241 gaatttagtg atagtataga gtaatcgtct ggatagttat cttcttgttt accattggca
     3301 gattgtgcca aagtttatta aacaaaccaa tcataatagt aggttagcgt ttctaagttt
     3361 ttgaaataaa agggggtgta accatgaaaa aaaaatattt tatctatttt gccatcctgg
     3421 cggttcttgc agcggtggcg tttgggggaa gaagcccggc ggttgaagat tttgccttca
     3481 gctgtgtcta tgggggagag cagtgcctgc cggtcgttga cactacagtc ggtatctgca
     3541 ggactctgca gtttgcaaca ccagatggcc tgataggagt attgctttgc cataaggatg
     3601 atttaaggta tgagctttac aggcaattct atccaggagc aaaagatttc aaagtatgca
     3661 cagcagatga tgcaggctgc atcgacagcc tcaggggatt tgacagcttt acaacacatg
     3721 aatttgactt tgagccaagc ccgactgact attgctataa gccgatagaa aacttccagg
     3781 tgacatgcgg agctccaggg tgccacgtta caaccgatac cactataggc acatgcaggt
     3841 cagttgtctt tgcaagcgat cttgggccag gacagatact agcatgccac aaaggagaca
     3901 acaggtatga ggtctattct cagcaaggcg cctataagag atgccttgga ccaggctgcg
     3961 tagaaggagc acaaggtttt gattcgtttg agataacatg cccgcctctt gcaggttaaa
     4021 ttttttttat tttttgtctc gatgttatag gggcgagttc tatcctaatt tcttgtacct
     4081 ctaaaagaat aacaattaca atagatacct tacacattca gcggaagtat aatgcatgtt
     4141 gctcctcttt tgaaaagctg ctcctggatc gcaaagacag tatagttgtg gagcactttt
     4201 gtcattgtcc ttacttcact aaatacctgc tggcccccaa aaaacactga gttgggaaat
     4261 cttgcaagta tttcaggcgc tatctttatg acttcactga cgacatctgt gccgacaata
     4321 gtgatagact cagcatagta gccgtacttg ttcatgaact ggatatattt ctgagattca
     4381 cacttgatgt cgcagtcaag ctgatttaac tcttgctggc ttttcatgag gcctgcttcg
     4441 attacaccta tcctcacaaa aacaaaattt ttgaagagcc cgccaaacga ttggaatatg
     4501 ttggcaaggg tctttagact cattccatta aatccgttca caaggacgac tgctgtcttt
     4561 tcataaggat gcacatattc atcgcttcta tcttcattat atactatggg aatctccggt
     4621 tgaggttttg atgactcagc tacatctatc agataatcaa gcttctgcag ccttatctta
     4681 cttttggtat agtgctgttt tatcctcatc gttaccaata tgagtatgag caacaggaaa
     4741 agcgtgatcc atccgcctga cttgaacttg actataagca tgcttccaag tatcagcacc
     4801 gaaatgagca gcccaagccc gttcaaaaag atccctttga tccaccctgc tatcttgttt
     4861 gcccaccagt aatggaccat cccaagctgg gaaaggcaga aagtgatgaa cacagatatg
     4921 ctgtataata atatcaagaa cccaatggaa cctcttgtga gtatcatcat aaaaagcgac
     4981 gcaaatccca tgataaggat gccgttttgg atgacgaacc tatcgcttag gacagtaaat
     5041 ctttttggaa gccatccgtc tcctgccata ttggcaagga tgcttggtcc cccaagaaat
     5101 cctgtctgtg cagcgacaaa aagcagtgcc gcttcgctaa tgagcgtgat aaggacaaaa
     5161 gccctgccaa gcaagggcgg ccatgatgag gcgatgttct caaaaaggac tgcattaagt
     5221 gtcttgccag gcacatgttc aactccaaac agtgtatagc ttatcattag tcctagaacc
     5281 agtatcacaa gagaccaggt catgtaaaac attgcccttc ttgcagtgag cacttttggc
     5341 tcccgaagta caggcatggc attgctcact gcctctatcc ctgtgtaggt tccagccccc
     5401 atgctgtatg catgtaaaag caggaataaa aacccaaaaa gcccaaggct cgagagcgct
     5461 gagctaacgt cccctgccgt cgcagtgatg gtctggctca cttgaggagc atgcgtaaat
     5521 aggccgtagg caagggcaaa gatatgggtg agaacaaaca gtgcaaatat aggcagcagt
     5581 gaaaatatgg attctcttac ccccctcaaa tttattacga taaggaaaac gatggccaaa
     5641 agggcaaaca taagtttcca ttgatggtat tcgacaggaa ggaaactaaa tatcgcgtcc
     5701 actccgctcg ctatcgagac agttatcgta agcacgtagt ctataagaag agcgcatcca
     5761 gatatcatcc cagcagtggg agacaacagt ttgcttgcaa cgatataccc accaccgccc
     5821 tgaggaaata gtttgactat ctgtgaatag cttgcgctta tgataagtat ggttgaagct
     5881 accatcagag cgatgaatac cgaaagatga gaatatttgc ctaatgcaag aaatgcctca
     5941 gcaggtccgt aggcagatga agaaagcgcg tcagagccaa gccctatcca ggcaaatatg
     6001 gcgatcagag agatcttatg aaaaagtaga ggatcctgga tatttcgggc cttgccgatg
     6061 atcaggtttt ttatcctttt ccgaaaatgt tggcgctttt ccttccgttg ctgcatataa
     6121 ggtcagtttt ccctggctat accgaaatct ctttatatgt gttacgttta aacaatgaaa
     6181 ttccccacat tgaacacttt agctaatttc gtgtaatctc ttttattaca ccgacgataa
     6241 ccattttata ggcatatcct tcttaggtta gtagttacct gattattata acattaaact
     6301 ccatttatgt gatatagaac aataatattt atatataagt agatacctga tatatgtatg
     6361 gcattccttt ttaagaagaa agtaaaggga atatactccc tttatatggg agagaacaaa
     6421 aaagtaaacg gcatatcaac aagggtcaaa tccaaatacc tagggccatt acacaggttc
     6481 aaagaatatt ttgctgatga tctcttcaag atagagcgca tcaatcatta tgagtatggc
     6541 ttatcctctg ccttaaagca aattatggac caattcctgt tccagaaaat attcagaaat
     6601 aaaataaaga aacaaaccga agatccctat atagacaggc tcatggtgct gatagttatg
     6661 aacaggcttg cagacccttg cgccaagtac agcttgcaaa gatggtttaa gggcacagac
     6721 cttgccaaca cctttaatat ccctgttgaa gaattagaag tgcacaaaat ctacagggca
     6781 atggaccagc ttgataagta cgcagaagag attgaaacag aaatatgcaa gacaatactt
     6841 atccaagaga acatatcgct taacatgatt taccttgatt ttacaaatca agagagctat
     6901 tcaagaaacc atgattcaga gctgctaaag aatgggcata acaaaagggg acatgatgag
     6961 ctgttgcagg ttaatctctc attaacctgc gattcaaata caggcatacc cttctttcat
     7021 aagagttacc caggcaatta caatgataaa caattcatca aaatctacgc agaggagtta
     7081 agagccaagc tggatgcgct gggctacaca ggaaaaaaca ccctgataat tgacaggggc
     7141 atcaatggag ataaagattt tgagttatta aggcagcata gatttgatta tatcggcggc
     7201 ttaatacaaa aagagttccc taaatatttt gaaatcccta agtcaaagct gaggaatacg
     7261 cacatccata caagagagac aaaagaagac gttaaaatca agtacacagg cattaaagag
     7321 gaaatatacg gccagaatca tcttatcctc actttataca atgttgagag ctatgatagc
     7381 gcaatagaag atttagacaa tagcctaaag gagtataaag tggcttgcga aaaacagctg
     7441 caggaattta agaaagagat tgcagaaaaa acatttcagt cgcactggaa taatgtcgat
     7501 aagataacga ataagctaaa agagataaac aaagagatct atcctctgct aagatttaag
     7561 attaaatctt ataggtttga cttaacttgg aaaattaacc agaataaaaa agaatacaac
     7621 aagtacgtcg ataattttgg aaaatatgta ttattcacca ataggcttga tttaaagcct
     7681 aaggaagtga ttgagttatt ctatgggaaa aagaagattg agaagaattt ccactatttg
     7741 aagtctaacg gctatacaaa caggtaccta aggatggggc ctatgcttca ttctaaggat
     7801 gagaggataa aaagccataa ttatacctgc gtatcggcgt tacagattta ccagataatc
     7861 aattataggc taaagaagaa ggggctggat atttctgcac aagaggcgct tgatgagcta
     7921 aagactataa catactacca tatcaaagtt cctgggcagg atgagcctat cagacatgta
     7981 aacctgctga ctcagaggca gaaggaaatt ctaaaggcat tggaagttga gctgtaagcc
     8041 tgcatttttt actcttgacg taaattacaa tttttgctcg aaagtgttca atgtgggaaa
     8101 ttccaccagt ctatttgact ggtggagtga attgacttag aaacagttgt ttctttagtt
     8161 cctagaaatc tgatggattt ctatgatgct caaaaaccac tttctggtag aacactaaaa
     8221 aatttgccat tgcaaatttt tggtgcacca aaaatcctat ggatttttta gtgtttcaag
     8281 cgtcaaaaac cacttaaccg tcatgccacc ag
//
LOCUS       DASRUK010000177         3587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_994176, whole genome shotgun sequence.
ACCESSION   DASRUK010000177 DASRUK010000000
VERSION     DASRUK010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994176"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VFF28_07665"
     CDS             complement(<1..519)
                     /locus_tag="VFF28_07665"
                     /inference="COORDINATES: protein motif:HMM:NF014749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HZX45530.1"
                     /translation="MDLLTLKDWSETDIKNIIDSAIKIKKSPEKYSSILKNKTMAMLF
                     EKPSTRTRISFEVAMTQFGGHALFIDWKTTQLAKAGLADEIKCIDRYVDIIMARVNKH
                     ETLEIMAKAAKKPVINALSDLYHPCQVLADLVTIKEKLGKLKGLKLAYFGDGNNVCST
                     LIVACKKVGMNIS"
     gene            730..1158
                     /locus_tag="VFF28_07670"
     CDS             730..1158
                     /locus_tag="VFF28_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45531.1"
                     /translation="MKKKKLMQFVTVFAVLAAFSTVSFGMISPQAQIVRAVEAIGNEE
                     DTYVRFVDGAFDSKVLNFLQVETVCALTTEQATEITKLYEYENRNFVVGEKDVNVLYL
                     KGNPLNKQRISESVGSLAPEDCQVVHGNKIVALISPVLVS"
     gene            1211..2326
                     /locus_tag="VFF28_07675"
     CDS             1211..2326
                     /locus_tag="VFF28_07675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZX45532.1"
                     /translation="MAYIWYRFILLFFTEIKHNKEQKAQYNNQLMSVIVPFYNEKLDI
                     LKKTIQSCLDALGNKEVIVVDDGSPDKTIAAAIQKEFKESIRFIRYEGNKGKRKAQAV
                     GAKFVKGEFIITVDSDTIIEKDALINLVNPMLCRREVAATTGNVEVLNLKENFLTRMI
                     ASRYWNAFNVERKSLSGFGIVTCCSGVLSAYRASLFKELIPLYVNQKFLGEECTYGDD
                     RHLTNLVLERKYRIEYVEDAICKTEVPNNMRQFIKQQLRWKKSFIRESIITLRFAFKH
                     SILLPVEVLFNLLIPFMGLAIRLSVVMTVILYPYLIPYFIFSVSTVAIIRNFFMFFER
                     KDIALFAIPYAFIHEFVIFWLYFVAVFQLKNSSWGTR"
     gene            complement(2328..3227)
                     /locus_tag="VFF28_07680"
     CDS             complement(2328..3227)
                     /locus_tag="VFF28_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45533.1"
                     /translation="MFDSFLNVISILLVMDRRLFLKLALASSVAGCTSVQRQPTATLD
                     DRIFTSDALVNNSLTLLPDHLLGAKAVHKYPVSQAKYCLIHIRQLHLGKNPSKKDYAK
                     VKKVQDRIYLVLNELISMYGLNRIYYEGVTEKREEDMNRSAQTSMLVRHGSKEANLEC
                     SKDDAFYHFERFRDYRAAYRIATDRPVHIVAAASSNLEEAANDYRHKTRGLGLLRLFI
                     DRTRAVNTIMEDREDFLLKKVSDNDDSVSVVVYGGQHAWGGKKSCGSAYYPWDRLSIK
                     DNIYCWNRLHPDKKFSLIEIACK"
     gene            3259..>3587
                     /locus_tag="VFF28_07685"
     CDS             3259..>3587
                     /locus_tag="VFF28_07685"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015590543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HZX45534.1"
                     /translation="MGKTIMVVGAQWGDEGKGKIVDFLAEQADVIGRATGGNNAGHTV
                     VVGNEKFKFHLLPSGTIHKGKLNICGNGMVIYPKQIISEIELLEKKGFKLNDKTLAIS
                     SAAHVIT"
BASE COUNT         1068 a          715 c          722 g         1082 t
ORIGIN      
        1 actaatattc atcccgactt tcttgcaggc tactatcaat gtgctgcaga cattgttgcc
       61 atcgccaaag taggctagct ttaagccttt tagcttacct aacttttcct ttatagtaac
      121 caggtctgct aaaacctggc atggatgata gagatcgctt aacgcgttta tcactggctt
      181 ttttgcagcc tttgccatta tctctaaggt ttcgtgcttg tttacccttg ccatgataat
      241 gtcgacatag cggtcgatgc atttgatctc atctgccagg cctgctttgg caagctgagt
      301 ggttttccaa tctataaaca gggcatggcc gccaaactga gtcatcgcaa cttcaaagct
      361 tatccttgtc cttgtcgatg gcttctcaaa aagcatggcc attgtcttat tcttcaatat
      421 cgaggaatat ttttcagggc ttttttttat cttgatggcg ctgtcaataa tatttttgat
      481 gtcagtttcc gaccagtctt ttaacgtaag taaatccatg gtagtaattc ttgcacaact
      541 tatttataaa tatattgacc gatgcaaaac aagaaagaca agaatgtcct aaatgggaca
      601 atgtattact ctttgtttga agattgaatt actaagacga caagcaagcc ctgattaaat
      661 agtcccttta atcattaaag ctaggccata aataacactg cttggcgaac ttaatataga
      721 ggtgataaga tgaaaaagaa aaaactgatg cagttcgtaa cggtattcgc cgtacttgct
      781 gcattttcga cagtaagctt tgggatgatt agcccccaag cacagatagt aagggctgta
      841 gaggccatag gaaacgaaga ggatacatac gtgcgcttcg ttgatggagc ctttgacagc
      901 aaggtgctca actttttgca agttgagaca gtgtgcgccc ttacaacaga gcaggcaaca
      961 gagataacaa agctgtatga atatgagaac cgcaactttg ttgtcggaga gaaggacgtc
     1021 aatgttctat atctcaaggg aaacccccta aacaagcaga gaataagcga gtcagtcggc
     1081 agccttgcac ctgaagattg ccaggttgta cacggaaata agattgttgc gcttatatct
     1141 ccggtgctag tgagttaaat tgagcttctt ttcttttaat ttttttatta aggtgtatgt
     1201 aatagtcacc ttggcatata tatggtatag attcatcctg ctttttttta ctgagataaa
     1261 acataataaa gagcaaaaag cccaatataa taatcaattg atgagtgtta tagtcccttt
     1321 ttacaacgag aaactcgata ttcttaaaaa gacgatacag agctgtcttg atgctctagg
     1381 aaacaaggaa gtgattgtcg tcgatgacgg ctcaccggac aagaccatag cagctgccat
     1441 ccaaaaggag ttcaaagaaa gtatcagatt tatcaggtat gaaggaaaca aaggcaaacg
     1501 caaagctcaa gctgttggag ctaaatttgt caaaggtgaa ttcatcatca ctgtcgactc
     1561 tgatactata atagagaagg atgcactgat caatcttgtc aatcccatgc tttgcaggag
     1621 ggaagtggct gcaacaacag gcaatgtcga ggtgttgaac cttaaagaaa atttcctgac
     1681 caggatgata gcatcccgtt actggaacgc tttcaatgtt gaaagaaaat cattgtccgg
     1741 ctttggcata gtcacctgct gcagtggagt gttgagtgca tacagggcaa gcctgttcaa
     1801 ggaattgatc cctttgtacg tcaaccagaa atttcttgga gaagaatgta cttatggaga
     1861 tgaccgccat cttaccaatc ttgtgctcga gagaaaatac aggatagaat atgtcgaaga
     1921 tgctatatgc aagacagaag ttcctaacaa tatgaggcag ttcatcaagc agcaactgag
     1981 atggaaaaaa tctttcataa gagagtcaat aataaccctg aggtttgcct tcaagcacag
     2041 catactcttg cctgtagaag tgctcttcaa cctattgata ccattcatgg ggcttgccat
     2101 aagattgagc gtcgttatga ccgtcatatt atatccttac ctgatacctt atttcatctt
     2161 ttcagtatcg acggttgcaa tcataagaaa ttttttcatg ttttttgaaa ggaaggatat
     2221 cgctctattt gcaataccct atgccttcat ccatgagttt gtcatcttct ggctgtattt
     2281 tgttgcagta ttccagctta agaacagctc ttggggaaca agataagtta cttacaagct
     2341 atctctatca gagagaactt tttgtctgga tgcaatctat tccagcagta aatgttatcc
     2401 tttatggata atctgtccca aggataatag gcgcttccac aactcttttt gccaccccat
     2461 gcatgttgac ctccgtaaac taccactgaa acgctatcat cgttatctga caccttcttt
     2521 agcaaaaaat cctccctgtc ttccatgata gtattaaccg ccctagtcct atcaataaac
     2581 aatctcaata aacctaaacc acgggttttg tgcctataat catttgctgc ttcctctaag
     2641 ttactgcttg cagctgcaac aatgtgtaca ggcctgtcgg ttgcaatcct ataagctgca
     2701 cgataatctc tgaatctttc aaaatgataa aatgcatcgt cttttgaaca ctcaaggtta
     2761 gcctcttttg aaccatgcct aaccagcatg ctggtttggg cgcttctgtt catatcttcc
     2821 tctctttttt ctgttactcc ctcataatat atcctattta gcccgtacat tgagattagt
     2881 tcatttagca ctaagtaaat cctatcctgc acttttttta cttttgcata atctttcttt
     2941 gatggatttt tacctaaatg taattgcctg atatgtatca agcaatattt tgcttgggaa
     3001 acaggatatt tatgtactgc tttagcacca agcaggtggt ctggaagaag ggttaatgaa
     3061 ttattcacta aggcatcgga cgtgaatatt ctgtcatcca atgttgctgt aggttgcctt
     3121 tgaactgaag tgcaacctgc tactgaagaa gcaagcgcta atttaagaaa taaccttcta
     3181 tccataacaa ggagaattga tattacattt aaaaaagaat cgaacaattt ataaatttct
     3241 tatttatttt cttatgctat ggggaaaact ataatggtcg ttggcgccca atggggcgat
     3301 gaagggaaag gaaaaatcgt tgatttcttg gcagagcagg cagatgttat cggaagggca
     3361 actggcggaa acaatgccgg ccatacggtt gttgtaggca atgaaaaatt taagtttcat
     3421 ctattgccat ccggcacgat acataaggga aagctgaaca tctgcggcaa cggcatggtc
     3481 atctatccaa aacagataat atccgagata gagcttcttg aaaagaaagg attcaagttg
     3541 aatgataaga cgcttgctat aagcagcgct gcccatgtta ttactga
//
LOCUS       DASRUK010000178         4900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1161416, whole genome shotgun sequence.
ACCESSION   DASRUK010000178 DASRUK010000000
VERSION     DASRUK010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161416"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="VFF28_07690"
     CDS             <1..1142
                     /locus_tag="VFF28_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45535.1"
                     /translation="KVGDRYCNGYSTACNGEWSWTSWSVKQDCNNEQYCMGGEGPNEL
                     SCTDIACTVDADCGPGSWGSTYCKDNDVYAVFTAASCQNGGTKESSCAYSNSEKRKQD
                     CGASGYSGSTYCWNYNGKPSVFQDYATKGCSDGSCFENTEKKLIKECVQGCTNGQCDE
                     DACQNDCGTGEARCEGTSLQTCGNYDADSCLEWIGDDCTDCSCSCGGFSVEESDSMNN
                     CNDNKDNDCDGAKDSLDAQCSIQFAISLNPGWNLISFPLLLNDKKIKDVFAGINFKSI
                     VSSNRYYYSEDNKSFELIDESDAYWVYSGSMQTLRISGRETDGGAKEGWSGYLSLHDY
                     TEPIEGYVIYMYNNSRWYTYNLERPERFNTLKRITPGYGYFIKKI"
     gene            1168..1926
                     /locus_tag="VFF28_07695"
     CDS             1168..1926
                     /locus_tag="VFF28_07695"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HZX45536.1"
                     /translation="MRLIDTGYNNAFMNMAIDEALISSPSPVLRFYGWKPAGLSLGYF
                     QDISQINTQFAKDNGIDVVRRLTGGNAVLHEHELTYSFIIDESLMPKSVLESYKAISR
                     GLLLGLRKLGIKAAMNEEVVPGKKSAVCFNDPSWYEIVVDGKKIVGSAQKRVGGKLLQ
                     HGAILIEKDVRKYCSCFHRSEELIARLEKRMTSINEVLGRKVSYEKVKMAMADGFKEA
                     INAEFILDGLSREETCLAIKLAKERYSSRQWNKT"
     gene            complement(1933..2676)
                     /locus_tag="VFF28_07700"
     CDS             complement(1933..2676)
                     /locus_tag="VFF28_07700"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZX45537.1"
                     /translation="MQPRKDRSLTEIQRGFDEMLYFVDAYQGKGPLNCLIVGLGHERV
                     YLDDPDIGRHLVGHGSYQPSIFAACCEGRGLDYNLTLLDKEYLPLYYARKMRKLVVLN
                     VPALDPAMPMYAELTCRKLSYSFFKTDIKAKLPKSLRKKRKAGEITYIQHDISQSITF
                     SNSPFDLIVCTNVLGYLHDPEKEKAALRTFRKNLSSKGVAIVSLHNIEVEDTGLADIT
                     EQILSKLRSSGKLHFGDAYHEYKLLTLRK"
     gene            2795..3193
                     /locus_tag="VFF28_07705"
     CDS             2795..3193
                     /locus_tag="VFF28_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008605262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HZX45538.1"
                     /translation="MTITLSFGTFDIIHPGHIYFLKKAKELGDRLVVVVARDSTIKEV
                     KKNSPKYNENQRLQHIKDLRIADEAMIGNEDDKYRVIEEIKPEIIALGYDQEHFAKDL
                     DKEMKKRGINAKVIRIDAYKKEQYKSSKLR"
     gene            complement(3180..3779)
                     /locus_tag="VFF28_07710"
     CDS             complement(3180..3779)
                     /locus_tag="VFF28_07710"
                     /inference="COORDINATES: protein motif:HMM:NF014015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45539.1"
                     /translation="MNVGAVISGGMNSWFAAYSAVSKGYDLTCMIAASRHPILGIQSK
                     AAQLPLISSDTLQGAIEIAKEKYGISGVVFGVINSTFKKEKIEDICKKAGVDCITPIW
                     HISDDLFLHQLMKNFHVIISGISAQGLKKEHLGLRVGLTFPDRMASLNISPSGEDGKF
                     DTIVLDCPLFQRKIVIEKSEARMENECKGTLNITRATLV"
     gene            complement(3813..4079)
                     /locus_tag="VFF28_07715"
     CDS             complement(3813..4079)
                     /locus_tag="VFF28_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45540.1"
                     /translation="MKCSNCKKEDQWLEIVGSDLICKKCINKHKIMSIVFRTHIDTFH
                     FLLRHLQPKNYQEFMKISRKEIEKLVNLKKYGIFLKTTNVVELE"
     gene            complement(4173..4883)
                     /locus_tag="VFF28_07720"
     CDS             complement(4173..4883)
                     /locus_tag="VFF28_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45541.1"
                     /translation="MQGAIPRCVDELFAEGIKTYLNAARCHKDSHEGNLWKTLEYPKD
                     IQSIFYEMAFNLYSRGSRSMSVEDICSSEKWNDIYKNTKSSVKQPFTKEERKALIREL
                     ALTSPFFGYSNIQDGQYPYQFTNLAVQEYMAGRELIRAIERLNTDRKQITVDYIAEAI
                     IKITDHDPLWWRQPLICACSDTDLGTHVMAFLLKTGIEDLIADAYFFGKHNPLDPKVS
                     DQFQGAYEKKYGPKKPTQ"
BASE COUNT         1432 a          955 c         1065 g         1448 t
ORIGIN      
        1 agaaggtcgg cgacaggtat tgcaatggat attcaactgc ctgcaacggc gagtggtctt
       61 ggacttcatg gagcgtaaag caagactgca acaacgagca atattgtatg gggggagaag
      121 ggccaaatga gctgtcatgc acagacatag cctgcactgt ggatgctgac tgcgggcctg
      181 gttcctgggg ctctacttac tgcaaggata acgatgtcta tgcggtgttt acagctgcaa
      241 gctgccagaa tggaggcacc aaggaatcct cctgcgcata ttcaaacagc gaaaagagga
      301 agcaggattg cggcgcaagc ggatatagcg gaagcacata ttgctggaac tataacggca
      361 agccaagtgt tttccaggat tatgcgacaa agggttgttc tgatggctcg tgctttgaaa
      421 ataccgagaa aaaacttatc aaggaatgtg tacagggctg cacaaacggg cagtgtgatg
      481 aagacgcatg ccagaatgac tgcggcaccg gggaggcaag atgcgagggc acttcgttgc
      541 agacatgcgg caactatgac gctgattcct gccttgagtg gattggcgat gactgcactg
      601 attgctcatg ctcttgcggt ggcttttctg tagaggagtc tgattcgatg aataactgca
      661 atgataataa ggataatgac tgtgacggcg caaaagacag cctggatgca caatgctcta
      721 tccaatttgc catcagctta aaccctggct ggaacctgat atcgtttcct cttctgctca
      781 acgataagaa gatcaaggat gtttttgcag gaataaattt taagtcgatc gtctcaagca
      841 acagatatta ttacagcgag gataacaaaa gctttgagct gatcgatgag tcagatgcat
      901 actgggtata ttcaggttct atgcagacct tgaggatatc aggcagagag acagatggcg
      961 gggcaaagga agggtggtca ggctacctta gcttgcatga ctatacagag cccatcgaag
     1021 gatatgttat ctatatgtat aacaattcca ggtggtatac ctataacctt gaaaggcctg
     1081 aaagattcaa taccctgaaa aggataacgc ccggttatgg atactttata aaaaagattt
     1141 aatagagcag ctgaattctt gtactttatg aggcttatcg atacagggta taacaatgcc
     1201 tttatgaaca tggctataga tgaggcactg attagctcac catcgcctgt tctcaggttc
     1261 tatggatgga agcctgccgg gcttagccta ggttatttcc aggatatctc ccagataaat
     1321 actcagtttg cgaaagataa tggaatagat gttgttcgca ggcttacagg aggaaatgct
     1381 gtccttcatg agcatgagct gacgtattct tttatcatcg atgaaagctt gatgcctaaa
     1441 agtgttttgg aatcctataa ggcgataagc agggggttgc ttttggggct taggaaactg
     1501 gggataaagg cagcgatgaa cgaggaggtt gtgcctggca agaaatctgc tgtgtgcttc
     1561 aacgacccat catggtatga gattgtcgtc gacggcaaga agatcgtcgg ctctgcgcaa
     1621 aaaagggttg gtggaaaact acttcagcac ggcgctatcc ttatagagaa agatgttagg
     1681 aaatattgct cttgttttca tcgcagtgaa gaactgatag ccaggcttga gaaaaggatg
     1741 acatctatca atgaggtgct tggcaggaag gtctcgtatg aaaaggtcaa gatggcgatg
     1801 gccgatggtt ttaaggaagc gattaatgct gagtttatac tggacgggct tagcagagaa
     1861 gagacttgcc ttgcgataaa attagccaag gagagatatt ccagcaggca atggaataag
     1921 acatgaatca tattattttc taagcgtaag cagcttatac tcatggtatg catcaccgaa
     1981 atgaagctta ccgcttgacc taagtttgga caatatttgc tcggttatat cagcaagtcc
     2041 tgtatcttca acttcaatat tatgcaaaga gactatggcc acaccttttg acgacaagtt
     2101 tttcctgaat gttcttaatg cagccttctc tttttctgga tcatgcaggt atccaagaac
     2161 attagtgcag actatcagat caaatggtga attggaaaaa gttattgact gcgagatatc
     2221 atgctggata taggttatct ctccagcctt tcttttcttc cttaggcttt taggaagttt
     2281 agcttttata tcagtcttga aaaagctata gcttaatttt ctgcatgtca attctgcgta
     2341 catgggcatt gcgggatcaa gtgctggaac attcaagaca acaagctttc tcatctttcg
     2401 tgcataatat aaaggaaggt attccttatc aagcaaagtt aggttatagt caagccctct
     2461 tccttcacag catgctgcaa atattgaggg ctgatatgag ccgtgtccaa ctagatgcct
     2521 tccgatatca gggtcatcaa gatacaccct ttcatggcca aggccgacaa tgaggcaatt
     2581 gagtggccct ttaccttggt atgcatcgac aaaatacagc atctcatcaa aacccctttg
     2641 tatctctgtc aatgacctgt cttttctagg ctgcatataa gaggagaaga tactgttatt
     2701 taagaaaccc tctgacgatt tttagattac ctattatatt tatgttgacg cctattgcga
     2761 tagattaata tatgattttt aattaacgat cgctatgaca ataaccttat cctttggaac
     2821 ctttgacata atccatccag gccatatata ttttctaaag aaggcaaagg aactcggcga
     2881 cagacttgtt gttgttgtcg cacgagattc tacgataaaa gaagtaaaga agaattctcc
     2941 aaagtacaac gaaaaccaaa ggctgcagca tatcaaagat ttaaggatag cagatgaagc
     3001 catgattgga aatgaagatg acaagtacag agttatcgaa gagataaagc ctgagataat
     3061 agcgctagga tatgaccagg agcattttgc aaaagacctt gataaagaga tgaaaaaaag
     3121 aggaataaat gccaaagtca tacggataga tgcctacaag aaagaacagt acaaaagttc
     3181 taaactaagg tagctcgggt tatattcaaa gttcctttac actcattttc catccttgcc
     3241 tctgattttt ctatgactat ctttctttga aaaagagggc agtcaagaac tatggtgtca
     3301 aactttccat cctctcctga cggtgaaata ttcaagcttg ccatcctatc gggaaatgta
     3361 aggccaactc tcaatccaag atgctctttt ttaaggccct gagcagatat gcctgagatt
     3421 atcacatgaa agtttttcat caactggtga aggaacagat cgtcgctgat atgccatatt
     3481 ggagttatgc agtcaacgcc tgcttttttg catatatctt caatcttttc ttttttaaat
     3541 gtggagttta tcactccgaa taccacccct gagatgccgt atttttcctt tgctatctcg
     3601 attgctcctt gaagggtatc tgaagatatc aatggcagct gtgctgcttt tgactgaatg
     3661 cccaggatag gatgtcttga tgctgctatc atgcatgtga gatcataacc cttgctcaca
     3721 gcagagtatg ctgcaaacca gctgttcatc cctcctgaaa ttactgctcc tacattcatt
     3781 ttaaatccag aacactattt actatttaat tgttattcaa gctctaccac atttgtagtt
     3841 tttaagaaaa ttccgtattt cttaagattg acaagctttt ctatctcttt cctgcttatc
     3901 ttcatgaatt cctgatagtt ttttggctga agatgcctta aaagaaaatg aaacgtgtcg
     3961 atgtgggtcc tgaagacgat gctcattatc ttgtgcttgt ttatacattt cttgcagata
     4021 agatcagaac ctactatctc aagccattgg tcttcctttt tgcaatttga gcatttcata
     4081 cttgctggtc atagtagggc aatatttaaa aagatatctt ttttcttctt ttaccactaa
     4141 gcgaaaactc aacaaagact aagccatgag atttattggg tgggcttttt tggtccatac
     4201 tttttttcat atgctccttg aaattgatca cttaccttag gatctaatgg attatgctta
     4261 ccaaaaaaat atgcatctgc tattaggtct tcaatacctg ttttcaataa aaatgccata
     4321 acatgagttc ccaaatctgt atctgagcaa gcgcagataa gtggctgtct ccaccaaaga
     4381 ggatcatgat ctgttatctt aataattgcc tctgcaatat agtcaactgt aatttgtttt
     4441 ctgtccgtat tgagtcgttc tattgctctg attaactcac ggcccgccat atactcctga
     4501 acagccaggt tggtaaattg ataaggatat tgaccatctt gtatattaga ataaccaaaa
     4561 aaaggagagg tgagcgctaa ttctcttatc aatgctttcc tttcctcttt ggtaaatggc
     4621 tgcttcactg aagactttgt atttttgtaa atatcattcc acttttctga cgagcaaata
     4681 tcttcaacgg acatgcttct gctgcctctt gagtaaagat taaatgccat ttcatagaag
     4741 atagattgaa tatctttagg atattcaaga gttttccaaa gatttccttc atgcgaatcc
     4801 ttatggcatc ttgctgcatt taggtaggtt ttgattcctt ctgcaaataa ctcatcaaca
     4861 caccgaggaa ttgctccctg cattacgcct gcgaggatta
//
LOCUS       DASRUK010000179         5978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2497406, whole genome shotgun sequence.
ACCESSION   DASRUK010000179 DASRUK010000000
VERSION     DASRUK010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 5978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5978
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2497406"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..604
                     /gene="rpl7ae"
                     /locus_tag="VFF28_07725"
     CDS             284..604
                     /gene="rpl7ae"
                     /locus_tag="VFF28_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7Ae"
                     /protein_id="HZX45542.1"
                     /translation="MADESDKIYEAIETAKATGKLRKGTNEVTKAIEKGEAKLVVTAK
                     DVSPPEIIMHIPLLCEEKGIPCVQVPSREELGAAAGIEKPTASVAIVVEGEAKNLIKE
                     ISGK"
     gene            666..932
                     /locus_tag="VFF28_07730"
     CDS             666..932
                     /locus_tag="VFF28_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011153348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S28e"
                     /protein_id="HZX45543.1"
                     /translation="MRGQQEEVKKGTVTFTEATPAKVEEVIGRTGTRGEAIQVRCKVL
                     SGRDQNKVLRRNVRGPVQVGDVLMLRETEIEARALSKAGRGSGS"
     gene            935..1105
                     /locus_tag="VFF28_07735"
     CDS             935..1105
                     /locus_tag="VFF28_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011019812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24e"
                     /protein_id="HZX45544.1"
                     /translation="MVKCTFCGENIKPGTGMMYVKSDATIMYFCGSKCEKNTLKLKRK
                     AHLVKWSGRYKK"
     gene            1107..1610
                     /locus_tag="VFF28_07740"
     CDS             1107..1610
                     /locus_tag="VFF28_07740"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF012554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - obsolete nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HZX45545.1"
                     /translation="MAVEQSLVLIKPDGLVKSLTGDVISKMAETGLKIVGAKIVQVTR
                     ELAEEHYQHLREEKFFDELIKYIMGEYHTRRVLALAYHGEDATKKIRKVVGATNPEKA
                     DPTSIRGKYGRITQAGVFENVVHASENPKEAEREIKLWFRPDELVYTMYPTEIRNVTI
                     DKVFWKS"
     gene            1747..3942
                     /locus_tag="VFF28_07745"
     CDS             1747..3942
                     /locus_tag="VFF28_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015791814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor EF-2"
                     /protein_id="HZX45546.1"
                     /translation="MGEKMIDRMGRIMRQPLQIRNICTSAHIHHGKTAFTDNLLAAAG
                     LMAETFAGDLDKGMVTWQHSDEQERLLTVDAANVSMVHEFQGKEYLINLIDTPGHVDF
                     GGNVTRAMRAIDGTIVLVCASEGIMPQTETVLKQALRERVKPVLFVNKVDRLINEMLF
                     TPEQIQERLIKIILEFNKLVEEIAEKDYGQKWKVNIQDGSVAFGSARENWALSLPYMK
                     KKGITFKDILAAYKMPEEEKKKWFWENSPLHEVILDMVIKHLPDPIAAQPYRIPRIWR
                     GELDSEMGQGLVKCNPNGKVAFCITRITIDPKSGREISAGRLFSGTLKEGQNVYINNI
                     ATPQRAQNVLMYIGIKPEAFKDIAAGNVLALAGVQGYAGDTVTDDPDNQPFEELKHIF
                     EPVITKSIEVTKPQDLPKLVEVLRKVAKEDPSIKIEIHEETGENLMSGMGELHLEIVE
                     NRIKTEKGLQVKTSDPIVVYRETITRKSAEVEGKTPNKHNKFYFYVEPLEPAIVDAIK
                     KGDLPEVRFKKKDLVVRDKLVELGMDSKTATNIKDIYKRNMFLDMTRGLVHISEAMEM
                     ILDMFENVMSAGPICKEPCVGVKVVLTDAAMHEDAIHRGPAQVYPAIRDGIRGAMMQA
                     GPILFEPYQIQEIEAPNEFMGEISKLISSKRGQLLEMEQEGNFVKIKAKMPVGELLGW
                     SSDLRSATAGRGSSSLVDQMFEKVPGELQVKIVKNIKDRKGLTDAMVGA"
     gene            4035..5315
                     /gene="tuf"
                     /locus_tag="VFF28_07750"
     CDS             4035..5315
                     /gene="tuf"
                     /locus_tag="VFF28_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor EF-1 subunit
                     alpha"
                     /protein_id="HZX45547.1"
                     /translation="MAKEKTHINLVFIGHVDHGKSTTVGRLLYDSKNIDEQAMRKLKD
                     KAKELGKVGFEFAFVMDNLKEEQERGVTIDLAHKRFDTPKYYFTIIDAPGHKDFIKNM
                     ITGAAQADAGVVVVDCSNGVQAQTKEHIFLSRTLGVHQIIIAVNKMDMVNYDQGRFNK
                     VKDEVSVLLKSVGYKPDTIKFIPEAALLGENVVNKSAKMPWYTGGTLLETLDTLSPPD
                     KPVNLPLRLPLQDVYNITGIGVVPVGRVETGIMKVGDKVIFVPAREGKGITGEVKTIE
                     MHHEQMQQAEPGDNIGFNVRGIEKKDIARGDVLGHVDTPPTVATEFTAQIVVLNHPTV
                     ITVGYTPVFHIHTAQVACQVMAILKKLNPATGEVLAENPDFIKNGDVAIIKVRPIQPL
                     VIERNKDIPEMSRFAVRDSGSTVAAGMCIDLVKK"
     gene            5370..5678
                     /gene="rpsJ"
                     /locus_tag="VFF28_07755"
     CDS             5370..5678
                     /gene="rpsJ"
                     /locus_tag="VFF28_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013905074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HZX45548.1"
                     /translation="MQKARIKLASTEISKINEVCEYIKDIATKTGVDMRGPIPLPTKK
                     LKVTTRKGPDGRGSSTWDRFEMRIYKRVVDLGLDERALRLVMRVPIPEGLNIEIEMVE
                     "
BASE COUNT         2030 a         1142 c         1500 g         1306 t
ORIGIN      
        1 aaaggttgag ttgtggcaga ccctcaaggg gcttgagatt cgcaaagaag gagtgaccta
       61 tggcacaatc acagattatt gggacaggtt tgagaaaaat gtatagttta gctgtcgcat
      121 ttcagagatg ttatagaaca ctagatgctt ctggcttcag tttaaaaaga caatcttttt
      181 aaacaggtta tatttctctt ttagttacct gtgattaatg aacccgaaag ggggaatgag
      241 gattagtttt ctagttcctc aattacaacc aaaggtgaaa aacatggcag atgaatcaga
      301 caaaatatat gaggctatag agacagcaaa ggcaaccgga aagctccgga agggcacaaa
      361 cgaagtgaca aaggctatag agaaaggaga agcaaagctt gtggtcactg caaaagatgt
      421 cagcccaccg gaaataatca tgcacatccc gcttctatgt gaagaaaaag gaatcccatg
      481 cgtgcaggtc ccatcaagag aagagcttgg ggcagcagca ggcattgaaa agcccacagc
      541 atctgttgct atcgtcgtag aaggcgaggc aaagaacctg ataaaagaga tctcgggaaa
      601 gtaggtgaaa cttgatggca gcaccaaaaa agccaatggc agcccctgag caacctgaaa
      661 aaaagatgcg cgggcagcag gaagaggtaa aaaaaggtac agtcactttc actgaggcta
      721 cgcctgcaaa agtagaggaa gtcatcggaa ggacaggcac aagaggagaa gctatccagg
      781 taagatgcaa ggtcctttca ggacgagacc aaaacaaggt cctaagaagg aacgtaagag
      841 ggcctgtgca ggttggagac gtattgatgc taagagaaac tgaaatcgag gcaagagccc
      901 taagcaaagc aggaaggggc tctggaagtt aaaaatggta aaatgcactt tttgcggtga
      961 gaatataaag cccggaacag ggatgatgta tgttaagagc gacgctacca taatgtattt
     1021 ttgcggcagc aaatgcgaga agaatacgct gaagctgaag agaaaggcgc accttgtcaa
     1081 atggagcgga aggtataaaa agtaaaatgg cagtcgagca atcacttgtt ttaataaaac
     1141 ctgacgggct tgtaaaaagc ctgacaggcg atgttatttc aaagatggca gagacaggcc
     1201 taaagatagt cggagcaaag atagtccagg tgacaagaga gcttgctgaa gagcattacc
     1261 agcatcttag agaagagaag tttttcgacg aactcatcaa atacataatg ggagaatatc
     1321 atactagaag agtccttgct ttggcctatc atggcgagga cgctacaaag aaaattagga
     1381 aggtagtagg agcgacaaac cccgaaaagg ctgaccctac ctcgataaga ggcaaatacg
     1441 gaaggataac ccaggcaggc gtctttgaaa acgtcgtgca tgcgtctgag aatccaaagg
     1501 aagctgaaag ggagataaaa ttatggttta ggcctgatga actggtctat accatgtacc
     1561 ctactgagat aagaaacgta acaatcgaca aagtattctg gaaatcatag aaattattag
     1621 ggcaaaaagt gcctatccca ccctaaaagg gggcgtgagg cccttgccca taatttctag
     1681 gacacaaaaa agccagaaaa aacaaaaagc cagagttggc ttttttcgtg taaggagagg
     1741 ataataatgg gagaaaaaat gatcgacagg atgggcagga tcatgagaca gcccctccaa
     1801 atcagaaata tctgtacaag cgcacacatc caccatggaa aaactgcgtt tactgataat
     1861 ctactagctg cagcaggcct tatggctgaa acctttgcag gagaccttga caaaggcatg
     1921 gtaacctggc agcattctga cgagcaggaa agacttctta cagtcgatgc agcaaacgta
     1981 tccatggtcc atgaattcca gggaaaagaa taccttatca atcttatcga caccccaggc
     2041 catgtagatt ttggcggaaa cgtcacaagg gcgatgagag ctatcgacgg aacgatagtg
     2101 cttgtttgcg cttcagaagg aataatgccg cagacagaga cagtccttaa gcaggcgctg
     2161 cgtgaaagag taaagcctgt gctgtttgtg aacaaggtcg acagactaat caacgaaatg
     2221 ctatttactc ctgaacaaat acaggagaga ctgataaaaa tcatccttga gtttaacaaa
     2281 cttgttgaag agatagcaga aaaagattat gggcagaagt ggaaagtaaa catccaggac
     2341 ggaagtgtgg cttttggctc tgctcgagag aattgggcat taagcttgcc ttatatgaag
     2401 aagaaaggaa taaccttcaa ggacatcctt gctgcataca agatgccaga agaagagaag
     2461 aagaaatggt tctgggagaa ttcaccactc catgaagtaa tacttgatat ggttatcaag
     2521 catctgcctg atcctatcgc ggctcagcct tacagaatcc ccaggatatg gagaggagag
     2581 cttgattcag agatggggca ggggcttgta aagtgcaacc ctaacggaaa ggtagctttc
     2641 tgcatcacca gaattacaat cgatccaaag tctggacgag aaatctcagc aggtcgtctt
     2701 ttctctggca cccttaaaga aggacagaat gtctatatca ataatatcgc aacacctcaa
     2761 agagcgcaga acgtactgat gtacatcgga atcaaacctg aagctttcaa ggatattgcg
     2821 gcaggaaacg tgctagctct tgcaggcgtt cagggttatg caggagatac tgtaacagat
     2881 gatcctgaca accagccatt tgaagagcta aagcatatat ttgaaccggt catcacaaaa
     2941 agcatcgagg tcaccaaacc gcaggacttg ccaaaactgg tcgaagtgct tagaaaggta
     3001 gcaaaagagg atccttctat caagatagag atccacgaag agaccggcga gaacctcatg
     3061 agcggtatgg gcgagcttca tcttgagatc gtcgagaaca ggataaagac agagaaaggg
     3121 ctgcaggtaa agacatctga tcccatcgtc gtctacagag agactatcac aagaaaatca
     3181 gcagaagttg aaggaaaaac accaaacaag cacaacaaat tttactttta tgtagagcca
     3241 cttgagcctg ctatcgtgga tgctatcaag aaaggggatc ttcctgaagt gaggttcaag
     3301 aaaaaagacc tggtggtgag agacaagctc gtcgaacttg ggatggactc aaagactgcg
     3361 acaaacatca aggatatata caagagaaac atgttcctcg acatgacaag aggccttgtc
     3421 catatcagcg aagctatgga gatgatactt gacatgtttg aaaacgtcat gagtgcaggc
     3481 ccaatatgca aggagccttg cgtgggcgta aaagtagtct tgaccgatgc tgcaatgcat
     3541 gaagatgcta tccatagagg ccctgcccag gtctatcctg ccatcaggga cggaataagg
     3601 ggcgctatga tgcaggcagg cccaatactt tttgagcctt atcagatcca agaaatcgag
     3661 gctccaaacg agttcatggg agagataagc aagcttatca gcagcaagag agggcagctc
     3721 cttgagatgg agcaggaagg aaacttcgtc aagataaagg caaagatgcc cgtaggagag
     3781 ctgcttggat ggagttcaga cctcaggtct gcaactgccg gacgaggaag cagctcactt
     3841 gtcgaccaga tgtttgagaa ggttcctggt gagcttcagg taaagatagt caagaacatc
     3901 aaagacagga agggacttac tgacgcgatg gtcggtgcat aaatcttttg caaataaaaa
     3961 gatttataaa caaaaagtaa attccagaat aggataaata tttataataa taataaacga
     4021 aaaggtggta aataatggct aaagaaaaaa cacacataaa cctggttttc atcggtcacg
     4081 tcgaccacgg aaaatcaaca acagttggaa gactactgta cgattctaaa aacatcgatg
     4141 agcaggcgat gagaaagcta aaggacaaag caaaagagct tggaaaagta ggctttgaat
     4201 ttgctttcgt catggacaac ctgaaagaag agcaggaaag gggagtcaca atcgaccttg
     4261 cacacaaaag atttgacacg ccaaagtatt attttacaat aatcgacgca cctgggcaca
     4321 aggacttcat caagaacatg ataaccggcg ctgcacaggc tgacgcaggt gttgtagtag
     4381 ttgattgttc aaacggagtc caggcccaga caaaggagca tatcttcctt tcaagaacac
     4441 ttggagtcca ccagataata atcgcagtga acaagatgga catggtcaac tatgaccaag
     4501 gaaggttcaa caaagtcaag gacgaggtaa gtgtactgct taagtctgtc ggatacaagc
     4561 cagacacaat caagttcatc ccagaagcag cacttcttgg agaaaacgtt gtcaataagt
     4621 cagcaaagat gccatggtac actggcggaa cacttctaga gacacttgat actctaagcc
     4681 cgccggacaa gccagtaaac ctgcctttga ggctaccttt acaggatgtt tataacatca
     4741 ctggtatcgg agttgttccg gtaggtagag tagagacggg tataatgaaa gtaggagaca
     4801 aggtcatatt tgtcccagca agagaaggaa aagggataac aggcgaagta aagacaatcg
     4861 agatgcacca tgagcagatg cagcaggcag agcctggaga caacataggc ttcaacgtaa
     4921 ggggcatcga gaagaaagac atagcaagag gagatgttct tgggcatgtc gacacacctc
     4981 caacagttgc aaccgaattt accgctcaga tagtggtgtt gaaccatccg actgtcataa
     5041 cagtgggata tacgccagta ttccatatac atactgcaca ggttgcttgc caggtgatgg
     5101 caatcttaaa gaagcttaac ccggcaaccg gcgaagtact tgcagaaaac ccagacttta
     5161 tcaagaacgg cgacgtagca atcataaagg tcaggccaat acagccactg gtaattgaaa
     5221 ggaacaaaga catcccagag atgagtagat ttgcagtcag ggattcaggt tcaacagttg
     5281 cagcaggaat gtgcatcgac ctggtgaaaa agtaaatatt ttttaccccg tttcatgaca
     5341 agccaaatgc ttgttcatga ggtaaaccaa tgcaaaaagc aagaataaaa ctagcaagca
     5401 cggaaatatc aaagatcaac gaggtctgcg agtacataaa agacatagca acaaagacag
     5461 gcgttgatat gcgagggcct atacctttgc cgacgaagaa acttaaagtg acaaccagga
     5521 aggggccaga tggtcgtggt tctagcactt gggacagatt tgagatgagg atctacaagc
     5581 gagttgttga tctcgggctt gatgaaaggg ctttgcgatt ggtgatgaga gtgcctatcc
     5641 ctgaaggtct gaatattgag attgagatgg ttgagtaaat tatttatttc ttatttgtta
     5701 gattgagatg gtgaataagg tttttaaata atcttgcttt attgcttaga tgggataaga
     5761 tttcttaaat aacccaagtt tgacagttag cgttttctga gttcactttt cgtatttttt
     5821 caggtttttt cacgcttggt gagaaggcgt tttttagcgt ttattagcca gatgaatttg
     5881 acagtttgac tgcttttttc agttttagct ccgaaagtca ggtagtagga tcaaaaagaa
     5941 gctaaaaacg ccttgatttc aggtgaaaac aaccagtt
//
LOCUS       DASRUK010000180         4402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_1166412, whole genome shotgun sequence.
ACCESSION   DASRUK010000180 DASRUK010000000
VERSION     DASRUK010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166412"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /gene="pgk"
                     /locus_tag="VFF28_07760"
     CDS             <1..251
                     /gene="pgk"
                     /locus_tag="VFF28_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HZX45549.1"
                     /translation="YSIEDDKVEKLSHTDKVTFMAAKACKDKGGITIAAGGDTVARIN
                     SRKAHDSFSTMTSAGGATLELINGSSAGRDAIKEALNQ"
     gene            complement(241..1473)
                     /locus_tag="VFF28_07765"
     CDS             complement(241..1473)
                     /locus_tag="VFF28_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45550.1"
                     /translation="MEKTEEAEAERLSLAELDGWLNVKIKSSKAQIDVKVEEITKNIS
                     SEIASTKANISTLISANLHNPKISVRETQFMEGNRSAYILAVNNFLRLIEFEDREPNE
                     LLNACNEFSSRLEKFGKNTVRPYHILQEFFANESRDIAINIKNIERLMIELKIAISPA
                     KLDRIETITALYLDIQGRSKRRNALEDGLEESKRLLDEMVKNRITTESELAELMKRKE
                     YKNFLELKAEKEAVLATIREENSMIINSFSAIERPLKKLDRMVAEDNRLLQKYVENCV
                     DALVFDKDLKIFAILGKLEQNINNLTLDLKDKKREKVLLSLKSITREYLTGFQNRRRE
                     LSDKLRSLEASIGQNQSHKEERKLLFDLETAGDDIERIQGEVNNLSSETAKCEIKESK
                     EKIAKEINECFGTNVFID"
     gene            complement(1531..1722)
                     /locus_tag="VFF28_07770"
     CDS             complement(1531..1722)
                     /locus_tag="VFF28_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008639817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZX45551.1"
                     /translation="MQNHIKKYRQLYKLRQSDLSEKLGVSRVTIIFIESSKHEPSLGL
                     AFKMARLFNCSIEELFDDK"
     gene            1833..2141
                     /locus_tag="VFF28_07775"
     CDS             1833..2141
                     /locus_tag="VFF28_07775"
                     /inference="COORDINATES: protein motif:HMM:NF015965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HZX45552.1"
                     /translation="MRAKLLKKLKAKKEARENIPKLLERAKKLYKQDLRLSNVYSKKI
                     KHLHMKYKLKLPKEIRRQLCKHCFSVLIPSVNSRVRLAKGRIIYYCSECKRYTRIPLR
                     "
     gene            2331..2486
                     /locus_tag="VFF28_07780"
     CDS             2331..2486
                     /locus_tag="VFF28_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45553.1"
                     /translation="MEELDEFEPRSQKGDFEYDEIRGAHGKKPKMAVLNKKKLSSYEM
                     DELFLEF"
     gene            2526..2765
                     /locus_tag="VFF28_07785"
     CDS             2526..2765
                     /locus_tag="VFF28_07785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0090560 -
                     2-(3-amino-3-carboxypropyl)histidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0017183 - protein histidyl modification to
                     diphthamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthamide synthesis protein"
                     /protein_id="HZX45554.1"
                     /translation="MYDLELEKAVAEIKRTKAKKVCIQLPEGLKPKAKEIADKLEKET
                     GADIFIWMGSCFGACDIPNIKGFDLLIQWGHSAWV"
     gene            2756..3652
                     /locus_tag="VFF28_07790"
     CDS             2756..3652
                     /locus_tag="VFF28_07790"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZX45555.1"
                     /translation="MGLSWFFFIMLAVIFWSVAAVIVKFIRIKFMRHPFSYMVITAPV
                     CFFVLLLLFFGKVDFPDAEVLLFIALSALSAFLGYWLYLTALRKEDVSTIVTLMGIQP
                     LIVLIFATVFLGETLSLKHYIAFPLMMVGSMALSIKKVDKRFICTSGILITLVSAILY
                     SLNSVFIKAIQQTDFITLNVLRQLAWLIFACAIFASSGTIRAHVKETVKAMGKKNLVL
                     AYVAEFLGFSGMIFAIVAIQKGPVSLVTLTSSLEGLFVIILAALVSLFIPRFIKENID
                     KKTICLKIFSALLMLTGIALVV"
     gene            3649..4026
                     /locus_tag="VFF28_07795"
     CDS             3649..4026
                     /locus_tag="VFF28_07795"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZX45556.1"
                     /translation="MKTFFVVTGIVQYENRYLLLKRSPDDYNYPNKWSFCSGFVKELE
                     AGEETVLREIKEETGLSAEIIKTGKVMEIIDSGKRWVVACYLCNAKEKKSSSAMKIQS
                     LSGQSLKILKNIDSYQACQEPPG"
     gene            4141..>4402
                     /locus_tag="VFF28_07800"
     CDS             4141..>4402
                     /locus_tag="VFF28_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009015484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HZX45557.1"
                     /translation="MATIIIGSDHAGFDLKEAIKAYLAEKGYGLDDQGAHSKDPVDYP
                     VIAKKVALKVASSGNIGILVCGTGIGMCMAANKVRGVRAALCY"
BASE COUNT         1263 a          883 c          922 g         1334 t
ORIGIN      
        1 cttactcgat agaagatgat aaggttgaga agctatcaca cactgacaag gtgacattca
       61 tggctgcaaa ggcctgcaaa gataaaggcg gaatcaccat cgccgcagga ggagatactg
      121 ttgcaaggat taactcaaga aaagcccatg attccttttc gacgatgacc agcgctggtg
      181 gagcgaccct tgagcttatc aacggcagct cagcaggcag ggatgcaata aaagaagcgc
      241 ttaatcaata aaaacattcg tcccaaaaca ttcgtttatc tcttttgcta tcttttcttt
      301 tgattctttg atttcacatt tggctgtttc gctgctaaga ttgttcactt cgccttgtat
      361 tctctcgata tcatcacctg cagtctctag gtcaaacaat agttttcttt cttccttatg
      421 gctttggttc tggcctatgc ttgcctctaa cgatctaagc ttgtcagaga gttccctgcg
      481 cctattttga aagccagtaa ggtattctct ggtgatggat tttagcgaca agagcacctt
      541 ttctcgcttc ttgtccttaa ggtcaagtgt gaggttgttt atgttctgct caagcttgcc
      601 aagtattgca aatatcttaa gatccttgtc aaatacaagc gcatcaacgc agttctctac
      661 atatttttgg aggagtcgat tatcttcagc aaccatccta tccagctttt ttagtggccg
      721 ctcgattgca gaaaaactgt ttattatcat tgaattctct tcccgtatcg tggcaagcac
      781 agcctctttt tcagctttga gttctaaaaa gttcttatac tccttcctct tcattagctc
      841 cgcaagctcg ctttcggtcg tgatcctgtt ttttaccatc tcatcgagga gtcttttgct
      901 ttcctcaagc ccgtcttcca gggcatttct tctcttgctc cttccttgga tatctagata
      961 aagtgctgtg atcgtttcta tcctgtccag ctttgcaggc gatattgcga tctttagctc
     1021 tatcatcagc ctttcgatgt ttttgatatt gattgcgata tctctgctct cgttggcaaa
     1081 aaattcctga agtatatgat atggcctgac tgtattttta ccaaactttt caagccttga
     1141 tgaaaattca ttgcaggcgt taagcagttc atttggctct ctgtcctcga attctatcag
     1201 ccttagaaaa ttgtttactg caaggatata tgcactcctg ttgccttcca tgaattgggt
     1261 ttctctgacg cttatcttag ggttgtgcag gtttgcggaa attagcgttg agatgtttgc
     1321 ttttgtgctc gctatctcgc tgctgatatt tttggttatt tcctccacct tgacgtcgat
     1381 ttgcgctttt gaagacttga tcttcacatt gagccatccg tcaagctctg ctagggacaa
     1441 tctctctgct tctgcctctt ctgtcttttc cataaacagt ttttttagaa aatttagcat
     1501 ggcaaggata atcccgcctt ttttataaat ttatttatca tcaaacagct cctcgattga
     1561 acagttgaaa agccgggcca tcttaaaagc aagaccaagg cttggctcat gcttggacga
     1621 ttctatgaag attatagtaa ctcgagacac tcccagtttt tcacttaagt ctgactgccg
     1681 cagtttatac aactgcctgt attttttaat atgattttgc aataagtagt atatttttaa
     1741 cattcaatat ataaaatttg ctataatgta taatttattt aacatatcac taaagcaaag
     1801 cttaataaat cactcaaaaa caaggaaagg atatgagagc aaagctgttg aaaaaattaa
     1861 aagcaaaaaa agaagcaagg gaaaatatcc ccaaattgct ggaaagagca aaaaagctct
     1921 ataaacagga tcttcgcctc tcaaacgtat attccaagaa gataaagcac cttcacatga
     1981 agtataagct aaagctgcca aaagagataa ggcgccagct ctgcaagcac tgtttttcag
     2041 tgttgattcc ctctgtaaat tcaagggtga ggctggcaaa aggtaggata atctattact
     2101 gtagcgagtg caaaagatat acccgtatcc cattaagata gaggattaac tactgttaaa
     2161 taacaacaga acgaaacctt taaatagtag ttataataag ctgtttctat gagccttttg
     2221 gaaaaacttg accaggaagc atctgtcagc gaagcaaaaa ggcagctgat agacaaagaa
     2281 gtaaaaaagc agagagagta tgaaaaatta ggctttatcg gtgagtaatt atggaagagt
     2341 tggatgaatt tgaaccaaga agccagaaag gcgactttga gtatgacgag ataaggggcg
     2401 ctcatggaaa aaagccaaag atggcagtcc taaacaaaaa gaagcttagc tcttatgaga
     2461 tggacgagct gttccttgag ttttgaccag caccttttta tataactatt ttctattaaa
     2521 ttttcatgta tgatcttgag ctggaaaaag cagtagcaga gataaagaga acaaaggcaa
     2581 aaaaggtctg catacagctg cctgaaggct taaagccaaa ggcaaaggag atagcagaca
     2641 aacttgaaaa agagacaggt gctgacatct ttatctggat gggctcttgc tttggcgcct
     2701 gcgatatacc taacataaaa ggttttgacc tcctcatcca atggggccat tcagcatggg
     2761 tttaagctgg ttctttttta taatgctggc tgtgattttc tggtcagtcg cagcagtcat
     2821 cgtcaagttt atcaggataa aatttatgag gcacccgttt agttatatgg tcattacagc
     2881 acccgtatgc ttttttgtcc ttttattgct gttctttggc aaggtcgatt ttccggatgc
     2941 agaagtgctt ctgttcatcg cactttcagc gctttcagct tttcttggct attggcttta
     3001 tctcacagct ttaagaaaag aagatgtctc taccatcgtc actttgatgg gcatccagcc
     3061 gttgatagtc cttatctttg caaccgtttt tctcggtgaa accttgtcgc ttaagcatta
     3121 tattgcattc ccattgatga tggtaggaag catggcttta tcgataaaaa aagtcgacaa
     3181 gagatttata tgcacaagcg gtatacttat caccctggtg tcggctattc tttacagctt
     3241 gaacagtgta tttataaaag ccatccagca gaccgacttc ataaccctca atgttcttag
     3301 gcagttagcc tggcttatct tcgcctgcgc aatatttgcc tcatcaggca cgataagggc
     3361 gcacgtcaaa gagactgtca aggccatggg gaaaaagaac cttgtccttg catatgttgc
     3421 agagtttctg ggatttagcg gcatgatatt tgcgatagtt gccatccaga aaggtcctgt
     3481 ctcgcttgtt acccttacct caagccttga aggcctcttt gtgataatac ttgcggcatt
     3541 ggtttcttta tttatccccc gcttcatcaa ggaaaacata gacaaaaaaa ccatctgctt
     3601 gaagatattt tcagccttgc tgatgcttac aggtattgca ttggttgtat gaaaaccttt
     3661 tttgtcgtga ctggaatagt ccaatatgag aacagatacc ttttgttgaa gagatcacct
     3721 gatgattata actatccaaa caaatggagt ttctgctcag gatttgtaaa agagcttgag
     3781 gctggtgaag agactgtctt aagggagata aaagaagaga ctggcctttc tgcagagata
     3841 ataaagacag gcaaggttat ggagataatt gacagcggca aaagatgggt tgtcgcatgc
     3901 tacctctgca atgcaaaaga aaaaaagtca agctctgcca tgaaaataca gagtttgtct
     3961 gggcaaagct tgaagatttt gaaaaatatc gattcgtacc aggcatgtca agaaccgccg
     4021 ggctgaagcc cggggcgtgt tcttgagcac aacataaatc caattgaggg tagatgattg
     4081 actagaggat ttctgttgtt acaaaagatt taaaaacatt gggcctgtaa agaagcatcg
     4141 atggcaacca taattatcgg ctcagaccat gcaggatttg acttaaaaga agcaataaaa
     4201 gcctaccttg ccgaaaaagg ctatgggctt gatgatcaag gcgctcattc gaaagatcct
     4261 gtcgattatc ctgtcatcgc aaagaaagtc gcattaaaag tagcatcctc aggcaacatt
     4321 ggaatccttg tctgtggaac aggcataggg atgtgcatgg cagcaaacaa ggtgcgtgga
     4381 gtaagagcag ccttatgtta tg
//
LOCUS       DASRUK010000181         4634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_820124, whole genome shotgun sequence.
ACCESSION   DASRUK010000181 DASRUK010000000
VERSION     DASRUK010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820124"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFF28_07805"
     CDS             <1..197
                     /locus_tag="VFF28_07805"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HZX45558.1"
                     /translation="PFDPSGLRMGTPSITTRGMKIGEMEIIGDWIASILKDPTNAHLK
                     KEIKAKVNRLCAEFPIYREF"
     gene            201..401
                     /locus_tag="VFF28_07810"
     CDS             201..401
                     /locus_tag="VFF28_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45559.1"
                     /translation="MYTPQNRQAPRRGSYQTPTYSGEYVAMYRGKVVAHDKGPIDVDR
                     VPEGSTIFFSINGDAVRQLTRR"
     gene            441..1286
                     /gene="folD"
                     /locus_tag="VFF28_07815"
     CDS             441..1286
                     /gene="folD"
                     /locus_tag="VFF28_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HZX45560.1"
                     /translation="MTAKIIDGQKIAEGIRADLKKKTSKAKTKPGLAVVLVGNNPASE
                     IYVNMKEKKSLEIGFVSKKVILPEETDTGELLETIDNLNQDPKIHGMLVQLPLPDHID
                     ENLIIGAILPHKDADGLHPLNLGHLLKGSNMIIPATPKGVMKLIDSTGVDIEGKHAVV
                     IGRSDLVGKPIALLLQQRNCTVTMCHSKTKDLTHYTKTADILVVAVGRPRMITKDMVK
                     KGAIVIDVGTNKVYDKLVGDVDFDEVKEVAGYITPVPKGVGPMTIACLLENTLECMEL
                     QDKML"
     gene            1343..2116
                     /locus_tag="VFF28_07820"
     CDS             1343..2116
                     /locus_tag="VFF28_07820"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HZX45561.1"
                     /translation="MKRYLGFLALLVLSSILVFAGIEQKPDRMVKIPKKAVEVEPGVF
                     YLGRAMDSGRVVEGYALVHYKEAKTKPGANSCYSFLASGARWKNIEPYMVLTDNSRGL
                     DSAFINDNLALDISKWETAAQKEIIGERIEGLVDGINMQSPDNKNEVLFGSISEPGAI
                     AITVVWGVFGGNPRARMLSEWDQVYDDVDFDWSSSGHPDKMDFESLATHELGHSVGLG
                     DLYNSACAAQTMYGYASVGEINKRSLESGDIKGVQTLYN"
     gene            2468..3070
                     /gene="purN"
                     /locus_tag="VFF28_07825"
     CDS             2468..3070
                     /gene="purN"
                     /locus_tag="VFF28_07825"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HZX45562.1"
                     /translation="MAVLGSTKGTDLQAVIDTLESGKLKGVEISFVLSDKKDAYILER
                     AKKHKLTAIFLDPKGKKREEYDKEVDSLLAKHGVQLILLIGYMKLMSSWFVDKWKNKV
                     MNIHPSLLPMFAGGMDKNVHQEVLKYGCKLSGPTLIFIDEGADTGPVILQKAVNIDEG
                     ETVDSLKEKVQKAEQEIIIKGIELFRDGKLKVEGRIVHII"
     gene            3143..>4634
                     /gene="purH"
                     /locus_tag="VFF28_07830"
     CDS             3143..>4634
                     /gene="purH"
                     /locus_tag="VFF28_07830"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HZX45563.1"
                     /translation="MKALISVSNKEGIVDLAKELINLKIEILSTGGTAKKLKDSGIKV
                     TDVSDYTGFPEMMEGRVKTLHPKIHGGLLALRSKKEHMAEAKEHGIEMIDIVVVNLYP
                     FEETAKKPNVKFEEIIENIDIGGPSMVRSSAKNFESVAIVVDPKDYPKIIKELKEKKK
                     VIEETRKALAVKAFQHTARYDSIISNYLNDKITKDPLPKSLTLAFDKLRDVRYGENPH
                     QRGAIYKDPFPKETSVINAKVPMEGKELSFNNYLDGDAAFELVKDFKEPTVAVIKHCN
                     PCGIASRDTIEKAFEDAYNVDPMSAFGCVIALNKRCTKQVAEMTKGKYIEVIIAPEFD
                     KEALNYIKENKQNIRLLETGPITKSNEGIAIKRVTDGILVETRDWPDIDKIELKVVTK
                     RKPSRQELEDLKFAWKVNKHTKSNSVVFCKDKTAYGLGVGQMSRVDASIIATRKSAGR
                     AEGGVMSSDAFFPFRDAVDEAAKAGIKAIIHPGGSVRDKEVIEAADE"
BASE COUNT         1573 a          868 c         1077 g         1116 t
ORIGIN      
        1 gtccatttga tccttctggc ctaaggatgg gaacgccttc tatcaccaca agagggatga
       61 agataggcga gatggaaata atcggcgact ggatagcaag catcctaaaa gacccgacaa
      121 acgctcatct aaaaaaagag ataaaagcaa aggtaaacag gctttgcgct gagtttccga
      181 tatataggga gttttaacct atgtataccc cacaaaatcg gcaagcgcca agaaggggat
      241 cttatcaaac tcctacctat tccggtgagt atgtagcgat gtatagggga aaagtggttg
      301 cccatgacaa aggacctatc gatgttgaca gggtgcctga aggaagtacg atcttcttca
      361 gcataaatgg agatgcagtg aggcaattaa caaggcgcta gagaaaaact ttatataacc
      421 ctttctttac ttttcatatt atgacagcaa aaataattga cggccaaaag atagcagagg
      481 gtataagagc tgacttaaaa aagaaaacat caaaggcaaa gacaaagcca ggccttgctg
      541 ttgtccttgt cggaaacaat cccgcttctg agatatatgt caatatgaag gaaaaaaaga
      601 gtcttgagat agggtttgtt tccaaaaaag tcattcttcc tgaagagaca gatacagggg
      661 aattgcttga aaccatcgac aatttaaacc aggatccaaa aatacacggc atgcttgtcc
      721 agctgccttt gcctgatcat attgatgaaa acctgataat cggcgcaata cttcctcaca
      781 aggatgctga tggccttcat ccactaaacc ttggccatct gctaaaagga agcaacatga
      841 taatccctgc aacaccaaaa ggtgtgatga agctcataga ttccactggt gtcgacattg
      901 aaggaaagca tgcagtcgtt attggaaggt cagacctcgt tggcaaacct atcgcactcc
      961 tcttgcaaca aagaaactgc actgtgacga tgtgccattc aaagacaaaa gatttgacgc
     1021 attacacaaa gacagcagac atccttgttg tagctgtcgg caggccaagg atgataacaa
     1081 aggatatggt caaaaaaggc gctattgtta tcgatgtagg cacaaacaaa gtttatgata
     1141 aattagtcgg cgatgttgac tttgacgaag taaaagaggt agcaggatac ataactcctg
     1201 tcccaaaagg cgttggtcct atgacgattg cctgtcttct tgagaatacg ttagaatgca
     1261 tggaactgca agataagatg ctgtaaagca aaatatttat agctataagt tctcaaccct
     1321 ttcataaatc gaggtgattg atatgaaaag atatttagga tttcttgctt tgttggtctt
     1381 atcttcgatc cttgtttttg ctggtattga gcagaagcca gacaggatgg ttaagattcc
     1441 caagaaagca gtggaagtgg agccaggagt gttttatctt ggaagagcga tggactctgg
     1501 cagggttgtc gagggctatg cgttagtaca ttacaaagag gcaaagacaa agcctggagc
     1561 taactcatgc tattccttcc ttgcaagcgg tgcaagatgg aaaaatatag agccatatat
     1621 ggtcctgact gataactcaa gagggctcga cagcgccttc atcaatgaca acctggcgct
     1681 tgacatttca aaatgggaaa cggcagccca aaaagagata attggagaaa ggatagaagg
     1741 gttggtagat ggtataaata tgcaaagccc agacaataag aacgaagtgc tgtttggcag
     1801 catctcagag ccaggagcga ttgcaataac cgttgtttgg ggagtatttg gtgggaaccc
     1861 aagagcaagg atgctttctg aatgggatca agtctatgat gatgtggatt ttgactggtc
     1921 gtcaagcggc catccagaca agatggattt tgaaagcctc gctacgcacg agctagggca
     1981 ttctgttggg cttggtgatc tatacaacag tgcatgcgct gctcagacaa tgtatggcta
     2041 tgcaagcgta ggtgagataa acaagagatc gcttgaatca ggcgacatta aaggagtcca
     2101 aacattatac aattaatttt ttctttttat aaaagaaaat agttgcatct ccaccaagca
     2161 ctaaactttt acaatttccc tcggtgagcc ataaagaata ttaagcaata atgctataga
     2221 tgagccatgg actacgttct tgtcggtggg gagtatcacc aagaaagagg cgttatgcat
     2281 gtccctgcag ccaaggcaga agcattatca tcgcaataaa agctatcgat gaatgccctc
     2341 aatggattaa gaattgcctg caatgtcctg aattcctatg tgtttgttaa gaacgcaaaa
     2401 gtttgaagca gtaaccttta tatacagcac agatttcttg gatgtacggt gatttgaaat
     2461 cctaaagatt gccgtacttg gaagcactaa aggcactgac cttcaggctg ttattgatac
     2521 ccttgagtca ggaaagctga aaggtgttga gatttctttt gtattaagcg ataaaaaaga
     2581 cgcttatatc cttgaaaggg caaaaaagca taagctgact gctatcttcc tcgatccaaa
     2641 aggaaaaaag cgcgaagaat atgacaagga agtcgattca cttcttgcaa agcatggtgt
     2701 ccagcttata cttctgatag gctatatgaa gctcatgtca agctggtttg tagacaagtg
     2761 gaaaaacaag gtaatgaaca tccacccaag ccttcttccg atgtttgccg gcggaatgga
     2821 caaaaacgta catcaagagg tcttaaaata cggctgtaag ctttcaggcc ctactttgat
     2881 tttcattgat gaaggagcag acacaggccc tgttattctc caaaaagctg tcaacatcga
     2941 tgaaggagag acagtcgact cgcttaaaga aaaagtgcag aaagccgagc aggagatcat
     3001 aatcaaaggt atagaattgt ttagagatgg taagttaaaa gttgaaggga ggattgttca
     3061 tataatataa aaatccctat cagggtattt ttaaagttat gataccctgt aaggacttag
     3121 gcaaaggagg ttatcataac agatgaaagc gcttatctca gtatcaaaca aggagggtat
     3181 agttgacctt gcaaaagagc tcataaacct aaagatcgaa atcttatcaa caggtggcac
     3241 agcaaagaaa ctgaaagatt cagggataaa agtgacggat gtttctgact atactggctt
     3301 tcctgagatg atggaaggaa gggtaaagac cttgcatcca aagatccatg gcgggcttct
     3361 tgccttaaga tcaaaaaaag agcatatggc agaggcaaaa gagcatggca ttgagatgat
     3421 agacatagtc gtcgtaaatc tttatccttt tgaagagact gccaagaaac caaatgtaaa
     3481 atttgaagag ataatagaaa acatagacat aggcggccct tctatggtaa ggtcctctgc
     3541 aaaaaacttt gagtcagtag cgatagttgt cgacccaaaa gactacccta agataatcaa
     3601 ggaactaaaa gaaaaaaaga aagtaattga agaaacaaga aaagcacttg cagtaaaagc
     3661 atttcagcac acagcaagat acgactccat aatatccaac tacttaaacg acaagataac
     3721 aaaagatcct ctgcccaaat ccctgacgct tgcatttgac aagcttaggg atgtaagata
     3781 tggagaaaat cctcatcaaa gaggagccat atacaaagat ccgtttccaa aggagacatc
     3841 cgtgataaat gcaaaagtcc ctatggaagg aaaagagctt tcgttcaaca attatcttga
     3901 tggcgatgca gcatttgagc ttgtcaagga tttcaaagag cctacggttg ctgtcataaa
     3961 gcattgcaat ccatgcggca tagcttcaag ggatactata gagaaagcct ttgaagatgc
     4021 atataatgtc gatcccatgt ctgcatttgg ctgtgtcatt gcactaaaca aaaggtgcac
     4081 aaagcaagtc gccgaaatga caaagggaaa atacatagag gtgatcattg cgccggagtt
     4141 tgataaggaa gccttaaact acatcaagga aaacaagcag aacatacgcc tccttgaaac
     4201 aggcccgata acaaaatcaa acgaaggcat tgctataaaa agagtcactg atggcattct
     4261 cgtagagacc agggattggc cggatataga caagatagag ctaaaagtcg tcacaaaaag
     4321 aaaaccttca aggcaagagc ttgaggattt gaagtttgcc tggaaagtaa acaagcatac
     4381 aaagtcaaac tcagtcgttt tctgcaaaga caagacagct tatggccttg gcgtcggcca
     4441 gatgtcaagg gttgatgctt caattatcgc cacaaggaag tcagcaggaa gggcagaagg
     4501 aggtgttatg tcttctgacg cattcttccc gtttagagat gcagttgatg aggcagcaaa
     4561 agcagggatc aaagcgataa tccatccagg cggctcagtg agggacaaag aagtgattga
     4621 agcagctgat gagc
//
LOCUS       DASRUK010000182         8005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_155524, whole genome shotgun sequence.
ACCESSION   DASRUK010000182 DASRUK010000000
VERSION     DASRUK010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 8005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8005
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155524"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VFF28_07835"
     CDS             complement(<1..512)
                     /locus_tag="VFF28_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012980198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45564.1"
                     /translation="MDKIQKRQVAYKTAISDILKADYVKEEGEWVPNYVKIGDKKVSR
                     VNILAVVVGKQNIENTNYQGMLIDDGSGKISVRSFERSFDLLNVGDFVLVIGRPREYF
                     NEKYIVAEIARKMENPLWAELRKIEINKVACTEEELAPVQEDAQEATPFSSETINQKI
                     FELIREIDSG"
     gene            complement(677..826)
                     /locus_tag="VFF28_07840"
     CDS             complement(677..826)
                     /locus_tag="VFF28_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45565.1"
                     /translation="MSEIASGARSDVSETAYGTYFAHALSMTHQAISLACSKTPYKQA
                     KMGSF"
     gene            complement(1031..4615)
                     /locus_tag="VFF28_07845"
     CDS             complement(1031..4615)
                     /locus_tag="VFF28_07845"
                     /inference="COORDINATES: protein motif:HMM:NF026240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HZX45566.1"
                     /translation="MEAQVEKFKDFIEKNYLSILNDNLRKDKRFLVIDFAELSMFNPE
                     LAEILLEQPEDTVRTAELAIEQLDLDNAKNVRVRFKNLPQSQFIQIRNIRSAHLNKLY
                     QMEGIVRQKSDVRPQVTAAKFECPSCGNIINVLQLDTAFKEPTRCGCGRKGKFNMISK
                     ELVDAQGLVLEESPESLDGGDQPKRMNVFLKDDLVSPFSERKTSPGAKIRVVGMIKEV
                     PIILRTGTKSTRFDLLIEANCVEAVEETFYEVGISEEEERQIKELSEDPKLIDKLIAS
                     IAPSIYGHDRIKEAILLQMMGGVHKKRKDGVQSRGDMHVLLIGDPGAGKSALLKRANI
                     IAPKARYVSGKGVSGAGLTAAVVKDEFLKGWSLEAGAMVLASNGLVCIDEMDKIGNDD
                     RAAMHEAMENQSYHPGTKIMLSDGTVHPIGDFVDKIIESNKSKVIPGKDCEMLPVNDI
                     ELLTTDFNKIFPVKAKMVSRHLPPEYFIKVTYSNGRSIAVTPEHPIFIYADGGIREIP
                     AENVRGGMLAPSPRNLPTRSKESSLDKGEAERHFQHLLDKDLARLLGYIITEGHSYNN
                     PSNRYAEIGVSNTNENVITEASELFSRVSGAHVNLNIRYAENTCHATKDLTTARVSSK
                     PFYSFMLNNFRGSINKARGKYVDNVIRCSKKENQLAFLSTAFKGDGFVDSTRCGFITS
                     SEELAQGYQDLLLQNGIWSRIDTECRLDKKYYKVAITGTQSFKKFNELIVEPDDKRKQ
                     RLRDFYSFSKRKRNERDILPRDMLIEINNMLKDFGASDGNLLTNIVRGYNAHKETVFD
                     YVDIIKERILSGERIIHEKDTKKIRKAIHIPVALLAKELKVSSSTIYNIEKNSGSKQY
                     FKLLSAARGMSARKIKNAKSKIGNIEQWRNSDIRCLKINKVEKVRDDSIRWVYDVTVE
                     PTRTFISEGLVLHNTVSISKANIQATLISRTTVLAAANPKFGRFDPYGIVAEQIDLPP
                     TLINRFDLIFTIKDLPDEKKDSMLASHILNLHQSPQYDDQEINTDLLKKYIGYAKRNI
                     QPKLSDGAIEEIKKYYLKMRSSGGGEDGVKTIPISARQLEGLVRMSEANARIRLCEEV
                     TRKDAKRAIAILEYSLMDVGFDKETGKIDIDRIATGVSASARNNISVVKEAINELEGK
                     IGKTIPIDDVVALCKEKGVSEEKVEEVLEKLKRTGDVFEPRHGFLSKI"
     gene            complement(4667..5224)
                     /locus_tag="VFF28_07850"
     CDS             complement(4667..5224)
                     /locus_tag="VFF28_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HZX45567.1"
                     /translation="MTNKKESKKRDIKVVNIVVSTSLEHDIPLEKMAATLSNTEYNPE
                     QFPGLVIRIKEPKTSALIFSSGKVVCTGARSMDKVHESIKKIIKSLEKIKIKITVKPE
                     IKIQNIVASGSVGMDLNLNVLAMKLENTEYEPEQFPGLVYKLEEARATFLLFSNGKIV
                     CTGTKSEEEVHSALDKLIENLKRVK"
     gene            complement(5356..6912)
                     /gene="cimA"
                     /locus_tag="VFF28_07855"
     CDS             complement(5356..6912)
                     /gene="cimA"
                     /locus_tag="VFF28_07855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HZX45568.1"
                     /translation="MIKIYDTTLRDGTQSPEVNFSVRDKLEIAKALDEFGADYIELGW
                     PGSNPKDREVFLEAAKLKLNKACVVAFGATRRKNISASEDENLKAIIQSKAKAACIFG
                     KTWIDHIDKQLKISAEENLVAIKDSVEFLKENRLYIFYDLEHFFDGYKDNPDYAMKCL
                     KTASDAGADCLVLCDTNGGCLPHEISLAVRQVKDFMRTKGIKKSLGVHFHNDSGTAVA
                     NCIAAAKEGVDEIQGTINGFGERAGNADLCQVIANLALKEGIKIGVEVEKLTELSKLV
                     YVLSNIKPFASQPYVGRNAFSHKGGIHVDAVMKGASYEHIDPNLVGNRRDIILSDLSG
                     KANIVEVAKKHGFIVSKDDKRTEAMLKDVEVLEKRGYDIGNLKAEQFLLVNKHFGNNK
                     GFFDIKTWKVMSEQRNGEFSECVITGSIDGKNREVVAPVEGGPVDSIFKALLKMTATN
                     YKQIKEIKLINYKVMIAEEQGAESSVRVYIEFRNGKEEWGTVGVSPNILEASLQAIEK
                     GFKYYLAKNC"
     gene            7139..7963
                     /locus_tag="VFF28_07860"
     CDS             7139..7963
                     /locus_tag="VFF28_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZX45569.1"
                     /translation="MLVEKDFLNKLRDFGLNTYESKIWTALLSRGVSTAGELSDIANV
                     PRSRSYDVLESLEKKGFILLKPGKPIRYVAVKPDEVIDRIKKKVKEETETQLNLIEKL
                     KSAGIVDALSTLHGQGLDLIEPTDLTGAIKGRRNIYNHLITMIKGAKKSVTIVSTAQG
                     IERKKDALFAHLAAAKQKGVKLRIAGPISPQDKCFKECSKLASVESTRLASRFVIVDK
                     KELLFMLMSDEDVHPGYDLAIWANTEFFSGFLEKAYDSLQANKSSMATDFPEVEHK"
BASE COUNT         2051 a         1856 c         1448 g         2650 t
ORIGIN      
        1 cttccgctgt ctatctcccg gattaactca aagatctttt ggtttattgt ttcacttgaa
       61 aatggcgttg cttcctgggc atcttcctgg acaggcgcaa gctcttcttc agtgcatgca
      121 accttgttga tctctatctt cctaagctca gcccataggg gattttccat cttccgggct
      181 atctctgcta ctatatactt ttcgttaaag tattctctag gtctgcctat caccaacacg
      241 aaatcgccca cattaagcaa atcgaaactg cgctcaaagc tcctaacaga tatctttcca
      301 gaaccatcat ctattagcat cccctggtag tttgtgtttt caatgttttg ttttcccacg
      361 accacagcaa gtatattcac tctagagact ttcttatctc cgattttgac atagttaggc
      421 acccattcgc cttcctcttt tacatagtct gcttttagaa tatcagaaat agctgttttg
      481 tacgctacct gtctcttttg tatcttatcc atccacccac cgaaagaaaa agtattagcg
      541 cttgtttaaa taacttactg agaagggggt tttattgccg atagattcga ttatgtcaag
      601 aaccgccggg ctaaaaagcc cggggcgtgt tcttgagcat gctagagaaa tcgctggctt
      661 actctgggct aaagtgtcaa aaactcccca tttttgcctg tttgtaggga gtttttgagc
      721 atgctagaga aatcgcttga tgcgtcatag acaaagcatg agcaaagtat gtgccatagg
      781 cggtttctga cacatcagag cgtgcgcccg aagcgatttc tgacatgtca aatagatgcc
      841 cctgtttcca atattactcc ccatctatga aaaaacaacc gggtggagac tggggaatgt
      901 ccgctggact ggaattaaat ttaactccaa tgccaatttc tgtagcaatc ggattaactc
      961 tctcttaggc ccaaacctct ttatcgggat atttgcctct cttaaatcat tagtgccaag
     1021 atatggcatc ctatatcttg ctcaaaaatc catgccgagg ctcaaacaca tcacctgtcc
     1081 tcttaagttt ctcaagaact tcctcaacct tctcttctga aacccctttt tctttgcata
     1141 gcgcgacaac atcatcgatt ggaatcgtct tgcctatctt tccctcaagt tcatttatcg
     1201 cttctttcac gactgagata ttattcctgg cagaagcaga gactcctgtt gctatcctat
     1261 cgatatctat cttacctgtt tctttgtcaa atccaacatc catgagtgaa tattccagga
     1321 ttgcaatagc tctctttgca tcctttctag tcacttcctc acaaagccta attcttgcat
     1381 tagcctcgct catcctgaca aggccttcaa gctgcctggc agagattgga atagtcttca
     1441 ccccatcctc tcccccgccg cttgacctca tcttaaggta atatttcttt atttcctcta
     1501 ttgcaccatc acttaatttt ggttggatat tccttttggc ataaccaata tatttcttca
     1561 gaagatcagt gtttatttcc tggtcatcat actgagggct ttgatgcagg ttcaggatat
     1621 ggcttgcaag catgctgtct tttttctcgt caggaaggtc tttgattgta aatatcaggt
     1681 caaacctgtt gatcaaggtt ggaggaaggt cgatctgctc cgcgactatg ccgtaaggat
     1741 caaaccttcc aaacttaggg tttgcagcgg caagtacggt tgttcttgag attagtgttg
     1801 cttggatatt cgcctttgat atgcttacag tgttatgcaa cacaaggcct tcactaataa
     1861 atgtccttgt aggctcaaca gtcacatcat acacccatct tatggaatca tctctcactt
     1921 tctcaacctt attaatttta aggcacctta tatctgaatt tctccactgt tcaatatttc
     1981 caattttaga ttttgcattt tttattttcc tggctgacat ccctcttgcc gcactaagca
     2041 gtttaaaata ttgtttgctg ccgctatttt tttcgatatt gtatatggtt gaacttgaaa
     2101 ccttaagctc tttagccaga agagctacag gaatgtggat agccttcctt atttttttag
     2161 tatctttttc atgaattatt ctttcgccgc tgagtattct ttccttgatg atgtcgacat
     2221 aatcaaagac agtctcttta tgggcattat atcctcgtac gatgttcgta aggagatttc
     2281 cgtcacttgc cccaaaatcc tttagcatat tgtttatttc gataagcata tctcttggga
     2341 gaatatccct ttcatttcgc tttcttttgc taaaagagta aaaatccctc agccgttgct
     2401 tccttttatc atccggttca acaattaact cattgaattt tttaaagctc tgggtgcctg
     2461 ttatagctac cttgtaatac tttttatcaa gcctgcattc tgtatcaatt ctgctccaaa
     2521 taccattctg gagcaataaa tcttgataac cttgcgctaa ttcctcagat gaagtgataa
     2581 atccgcatct ggtagaatcc acaaatccat ctcccttaaa tgctgtagat aaaaatgcaa
     2641 gctggttctc ttttttgctg caacggataa cattatcaac atattttccc ctggctttat
     2701 tgatgctccc tctaaaatta tttaacataa agctatagaa tggcttggag cttactctag
     2761 ctgtagtcaa gtctttggtg gcatggcagg tattttctgc atatcttata tttagattta
     2821 catgggcccc agatactctg ctaaatagct cgcttgcttc ggtaataaca ttttcattgg
     2881 tgtttgatac tccgatttct gcatatctgt tcgagggatt attatatgaa tggccttcag
     2941 ttatgatata gccaagcagt cttgccaagt ccttgtcaag taaatgctgg aaatgccgtt
     3001 ctgcttcccc cttatctaag gagctttctt tcgatcttgt cggcagattc ctgggggaag
     3061 gagcaagcat accccctctc acattttctg ctggaatttc ccttatccct ccgtccgcat
     3121 agataaaaat tggatgctca ggggtaacag caatagacct gccatttgag tatgtgacct
     3181 ttataaagta ctcaggtggc aggtgcctgc ttaccatttt tgcctttact ggaaatatct
     3241 tgttaaaatc agtcgtgagt aattcaatat catttacagg tagcatttca caatctttgc
     3301 ccgggattac cttggactta ttagattcaa ttattttgtc aacaaaatct ccgataggat
     3361 gaacagttcc atctgagagc attatctttg tcccaggatg atagctctgg ttctccatag
     3421 cctcatgcat cgcagccctg tcgtcatttc ctatcttatc catctcatct atgcatacca
     3481 gcccgtttga agcaagtacc atcgctcctg cctcaaggct ccatcctttg agaaattcat
     3541 ctttgacaac agcagcagta aggcctgcac cggacacgcc tttgccgctg acatatctcg
     3601 cttttggtgc tattatattt gctcttttta gaagtgcgct ctttccagct ccaggatccc
     3661 ctattaaaag gacatgcata tctcctctgc tctgaacacc atctttcctt tttttatgca
     3721 ctccgcccat catctggaga agtatcgctt cttttattct gtcatggccg tatatagaag
     3781 gagctattga agctatcagc ttatcgatga gttttggatc ttcagacagc tcttttatct
     3841 gcctttcctc ttcttcgctg atgccgactt cataaaaagt ttcctccaca gcttccacac
     3901 aatttgcttc tatcaaaagg tcaaagcggg tagactttgt tccggtccta agtattatcg
     3961 gcacttcctt tatcatcccg accacgcgta tctttgcacc aggagatgtt ttcctttctg
     4021 aaaacgggct gaccagatca tccttcaaaa aaacattcat cctttttggt tgatcgccgc
     4081 catcaaggct ctcaggtgat tcctcaagga caagcccttg tgcatcgacc agctctttgc
     4141 ttatcatgtt gaattttcct ttcctgccgc acccgcacct tgtaggctct ttaaatgcag
     4201 tgtcaagctg gaggacattt atgatattgc cgcacgatgg gcattcaaat ttagctgccg
     4261 taacctgcgg cctcacgtct gatttttgcc ttactatccc ttccatctga tagagtttgt
     4321 ttagatgtgc gcttcttatg ttccttatct gtatgaattg tgactgcgga agattcttaa
     4381 accttaccct gacatttttg gcattgtcca ggtcaagctg ttctatggca agttcagctg
     4441 tcctcaccgt atcctctggc tgctcaagaa gtatttcagc aagctcagga ttgaacatag
     4501 agagctcggc aaaatctatg acaagaaacc tcttgtcctt tcgaagatta tcgttcaaaa
     4561 tagaaagata gtttttttca atgaaatcct tgaatttctc gacttgcgct tccattttta
     4621 aggtagggct ctataggaga cgctgctacc ttaagaaact tgccctttac ttgacccttt
     4681 ttagattctc tataagcttg tcaagcgctg aatggacttc ttcttcgctc tttgttcctg
     4741 tgcatactat cttgccgttg gaaaacaaca ggaaagttgc ccttgcttct tcaagtttgt
     4801 agactagccc aggaaattgc tctggctcat actcagtgtt ctcaagcttc attgcaagca
     4861 cgttcaggtt taggtccatg cccacactgc cagatgcaac tatgttctgt atctttatct
     4921 ctggtttgac agttatcttt atcttgattt tttcaaggct ttttatgatt ttcttgatgc
     4981 tttcatgcac tttatccatg gatcttgcac cagtacagac cactttgcca gagctaaaga
     5041 taagagcgct tgtttttggc tcttttatcc tgataacaag gccaggaaac tgttctgggt
     5101 tatattcagt attagaaaga gtcgctgcca tcttttcaag gggaatatca tgctctaaag
     5161 atgtagaaac aactatattg actaccttta tatctctctt cttagattcc ttcttattag
     5221 tcataaaagc acccccaagt gacctctttt tatcctaata atgctttaat tgcctttatt
     5281 agcactattt tgatatattc ctttataaat aggcagagta tttaaatact ttcatttata
     5341 aacaaagaaa accagttagc agttctttgc cagataatac ttaaatcctt tttcgattgc
     5401 ttggagtgat gcctctagta tgtttggtga tactcctaca gttccccact cctctttgcc
     5461 gttcctaaat tctatataga ctctaacaga gctttctgca ccttgctcct ctgctatcat
     5521 gactttatag tttatgagtt ttatctcctt tatctgtttg tagtttgtcg cagtcatctt
     5581 caggagagcc ttaaatatcg agtcgaccgg tcctccttct actggagcga ctacttccct
     5641 gttttttcca tcgatcgatc cagtgatcac acattctgaa aattctccgt ttctttgctc
     5701 agacataact ttccaggtct ttatatcgaa aaaccccttg ttgtttccaa aatgtttgtt
     5761 tacaagcaaa aactgctctg ccttgaggtt acctatatca tatcctctct tctcaagcac
     5821 ctcgacatcc tttagcatgg cctctgtcct tttatcatct tttgatacga taaagccatg
     5881 cttttttgca acctcaacta tgtttgcttt gcctgacagg tcgctgagta tgatatctct
     5941 tcgattgccc acgagatttg gatctatatg ctcataggat gctcctttca ttacagcatc
     6001 gacatggatt ccacctttat gcgaaaaagc gtttcttccg acatacggtt gtgatgcaaa
     6061 cggtttgata tttgacagta catatacaag cttgctgagt tcggttaatt tctctacttc
     6121 cactcctatc tttattcctt ccttcaaagc aagattagcg atgacttggc ataggtcagc
     6181 attccctgcc ctttccccaa atccgtttat tgttccctgg atttcatcaa ctccttcctt
     6241 tgcagcagct atgcagtttg caacagcagt cccagaatca ttatggaaat gtacaccaag
     6301 actttttttt attcccttgg ttctcatgaa atccttcact tgcctgactg ctagagagat
     6361 ctcatgaggc aggcagccgc cgttggtatc gcagagcacc aggcagtcag ccccggcatc
     6421 agaagcagtc ttaaggcatt tcattgcata atcaggatta tctttatacc catcaaaaaa
     6481 atgctccagg tcataaaata tatacaatcg gttctccttc aaaaattcta cactgtcctt
     6541 gatagcgaca aggttttctt ctgcacttat cttcagctgt ttgtctatgt ggtctatcca
     6601 tgttttgcca aatatgcatg ctgccttggc cttgctttgg attatagcct tcaaattctc
     6661 atcttcggat gcagatatat ttttcctcct ggtagcgcca aatgcgacaa cacatgcttt
     6721 gtttagctta agttttgcag cttcaaggaa cacttcccta tccttagggt tcgagccagg
     6781 ccatcccagc tctatataat cagccccgaa ttcatcaaga gcttttgcta tctccagctt
     6841 gtcccttact gagaagttta cctctgggct ttgagtccca tcccgaagtg tggtatcata
     6901 gatttttatc atcttatcct cgttggctat aaccccgtta ggaattatag cttatgctat
     6961 aataatgttc agaataaccg ctaagcatac ctgatgcttc atggcttaaa gaatttgccg
     7021 cttcctatat aaatctttgc cttgaaaaaa tatttgtcac ttaagaatag ttaattatag
     7081 aaacctttat ataaggtaaa ccattccgat aatctaaaag aggttctcag gtgatataat
     7141 gctggtagaa aaggattttt taaacaagct tagggatttt ggtcttaata cttatgagag
     7201 taagatatgg acggcgcttc tttcaagggg cgtttccaca gcaggagagc tctctgatat
     7261 tgcaaacgtt ccaaggtcaa gaagctatga cgttctcgaa agccttgaga aaaaagggtt
     7321 tatcttgcta aagccaggaa agcccataag atatgtagca gtaaaaccag acgaggttat
     7381 cgataggata aaaaagaagg taaaagaaga gactgaaact caattgaatc ttatagaaaa
     7441 attaaaatca gcagggattg tcgatgccct tagcactctt catggccagg gccttgatct
     7501 tatagagcct actgatctta caggcgcaat taagggaaga agaaacatct acaaccatct
     7561 cataaccatg ataaaaggtg caaaaaaatc agtcactatc gtttcgacag cgcaaggtat
     7621 tgagagaaaa aaagacgcct tgtttgccca tcttgcagca gcaaagcaaa aaggcgtaaa
     7681 acttaggata gccggcccaa ttagccctca ggacaagtgc tttaaagaat gctcaaagct
     7741 tgcaagcgtt gagtccactc gcctggcctc aagatttgta attgtcgaca aaaaagagct
     7801 gttgttcatg ctgatgagcg acgaggatgt ccatccaggc tatgatttgg caatatgggc
     7861 aaacacagaa tttttctcag gatttcttga gaaggcctat gactcccttc aggcaaataa
     7921 atcctcgatg gctacagact tccccgaagt agagcataaa taatattaag gagtgtatca
     7981 atagattaag acgttgggag gtaag
//
LOCUS       DASRUK010000183         7702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2000276, whole genome shotgun sequence.
ACCESSION   DASRUK010000183 DASRUK010000000
VERSION     DASRUK010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 7702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7702
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2000276"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..911
                     /locus_tag="VFF28_07865"
     CDS             195..911
                     /locus_tag="VFF28_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45570.1"
                     /translation="MRRYTGISTFKEYPQRAGIDEVEYNGHFFGIRDELDKRVFCISY
                     LPKRPVFSAISNIHELRHGLQKERQVMVEVRKNFTSLPNFQRLEAKVKASRGLVKRRF
                     DFMRNTHGLNFYKSPDVRKLAQESGMDISAYDAFLAENVPFMTLIQCYCEGDAHVMEP
                     VITPLIPQLEGQSLVLDMYILSAYIDCLLAHAKSKMQKVYWVGTGIAEYIRNHPPEKD
                     AILGSKEFQKQLTDDFFTKK"
     gene            982..1431
                     /locus_tag="VFF28_07870"
     CDS             982..1431
                     /locus_tag="VFF28_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45571.1"
                     /translation="MLTGIFGSRGSVNLSLLSHAVRNQPSLIVDCANIANPHVLFPFA
                     QPEDMHDIYVVQAELMYKFRDILKHLDRLAKQVNPRCIVITLFDRLFHYNDEQENKDI
                     IEHAWELMKKHSERYELIAGANHPLASNFCDNMIGVEEWDTLCLARG"
     gene            1401..1622
                     /locus_tag="VFF28_07875"
     CDS             1401..1622
                     /locus_tag="VFF28_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45572.1"
                     /translation="MGHTVLSQRIVADLIIKELNEYGKSLRGKDREIFNRLLKEPLKH
                     IGSISYANSMHAWAFLIISMLIEMEKKNG"
     gene            complement(1628..1780)
                     /locus_tag="VFF28_07880"
     CDS             complement(1628..1780)
                     /locus_tag="VFF28_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45573.1"
                     /translation="MEHKTFSNRGNRFETENMVSDTVQSEFDSLFETSNMVDRINREK
                     RESIAI"
     gene            2755..5331
                     /locus_tag="VFF28_07885"
     CDS             2755..5331
                     /locus_tag="VFF28_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HZX45574.1"
                     /translation="MKAWVMDAYRKENSIVLWLKTPENDFTVTRSFNHYIYLDIESKE
                     FLDSKKIKYRGVRKKDYMRREISVLEVKVPWLSNFEAFVRYLEKETRHRVPFYNADIT
                     PEQMFFYKNDFLPCSTVEVDEGSIRPVLGETPVLSKMELKVNAEKDIYLNPDCKVVSI
                     IVDSEKIEGDEVHVLSTFKQRFLSKDPDIIHAQFAFSRIPFLMRRFLANGIDFSFHRF
                     DDVPLKYKGGRTFFTYGISRYREFAVRLHGRFLVDNSSFMGGACEADAIMELSQLSGM
                     FLQQIASKSFGAAFQFRLIKEMVSQNLLVPFKEKPIEQPLSMLELLKTDRGGFTADPT
                     VGFHEDVAELDFCSMFPWLIYNHNVSADTILSDEGPFEHVPGTPFKISLRHKGLAAAG
                     IKKFLDRRMEYKRNPTAVNKAKAAGLKWVLVSSYGYLRFREFKLGVSSAHMSICAFAR
                     EILVKLSRVAESMGFEVVHSIVDSIYIKKKGITEKEVKEFCREIELETGIPISVEGIF
                     KWIVFLPSINDNARPVPTRYFGVYRNGEIKARGIEVRQSSCPLIIKEFQKECLRVLGK
                     CSGKMEIRESIPELYKLMEEYSKKVGKAPAALLVSALRISKEDYKNDIPQRTIISSLK
                     KKGITIRAGEYINFVFSSRGPVIPEDYSGNPDFSHYRKLFIRSLYSILQPFGVEREKI
                     SFKYDRQKTLSDYMSVKNVYIPMLSQPPEKRGLSEKIIKKRLVLQGWTVWKGGLLGIE
                     KVSEYPNFIRKYILLKQLLSKVDEKLPEELEYMSNVHHGMPDYICYRNGKFKFVECKL
                     QYELLSDKQKKCLMKLKGMGFDVEVHRLVDRRTKAREAMVDVVNGGKMIMEVQNRLVT
                     PH"
     gene            5380..6030
                     /locus_tag="VFF28_07890"
     CDS             5380..6030
                     /locus_tag="VFF28_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45575.1"
                     /translation="MAKEDSTPIPLTKKDIANLLSGKAFERLLVKSSRYTARTGQTSG
                     FYATRDILGQCHFSKVDTEGYASNSLSIKVNDEMGKYPLMHLQFCTNNGYISPPGRGD
                     LLAYTLLKNQGAYQSRPINILAQVDDLGNGELVLLQEKDVETLSAADLSIICQKTRHL
                     IQGEHSAEEIAKAFQNTGYYTSTTMRFQLHKRNALIPEPDIRRACKFAYTPVMRPG"
     gene            complement(6027..6563)
                     /locus_tag="VFF28_07895"
     CDS             complement(6027..6563)
                     /locus_tag="VFF28_07895"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZX45576.1"
                     /translation="MREQKFTQIKAAYDDYYKELLRNGKLPLRSTELGFWNAAITGEV
                     FEAFRKLKLSKKSSFIDLGSGDGKVTLLASLFCSRAEGIEIDDELHHKALEIKDKLGI
                     SNVVFHHDDFNEHSIEGYDIVFINPDKPLQRGLEKKLLNELSGKLILFGHHFHPSNLV
                     KEKEVVVDNTLVAVYNRF"
     gene            complement(6560..7042)
                     /locus_tag="VFF28_07900"
     CDS             complement(6560..7042)
                     /locus_tag="VFF28_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45577.1"
                     /translation="MPKINKEELDKLKPAEKIKKLKELEEESKKEIEEAEKLIKQTQA
                     ELEKQGIAESVKVPETKPIDIESLFKVEPGLEGTVKKSAPAEESSSQLYLLAHVYEAA
                     KGLYESEEPLSNRQLEWIDKLGEKVEKISYRVASKEVADLVVATRSIVRKIRQYHTNI
                     "
     gene            complement(7047..>7702)
                     /locus_tag="VFF28_07905"
     CDS             complement(7047..>7702)
                     /locus_tag="VFF28_07905"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZX45578.1"
                     /translation="IVDFLTKERVKFGITEKGDYPNNKDGISPYYQPSLNKEEAEKLA
                     EKYIKATFDTGHANTWRKYWQIKPGQTIEDADKEFKQWYTKEFEKLVKNGYVGNIHLT
                     DNFGYHDDHVAPGQGNAPIREVMDILKKYKYDKAITVEPGADASTDLSDFHGLMKTWR
                     YLGSPIYGTGAGGMARIPQSWSDVHYSYFGQSQPPMFTFGAYAPSNDWTLWSQVPME"
BASE COUNT         2169 a         1550 c         1661 g         2322 t
ORIGIN      
        1 ctgtaatttt gttttgtatc ccatagtatc accttgttaa ctatatatac atatagttaa
       61 taaatttttc gttttatgaa tagagtgcat aatctgagta aaagattaga atttaccgat
      121 tcctagtttc cgcacaccct cctgagtata ggcccagaga tatacatgaa gcgctttgtt
      181 atgctgacta tgcaatgagg agatatacag gcatctcaac ttttaaggaa tacccgcaaa
      241 gggcgggaat agatgaagta gagtacaatg ggcatttctt tggaatcagg gacgagttgg
      301 ataaacgagt cttttgcatc tcttaccttc ctaaaagacc tgtgttctca gcaataagca
      361 atatacatga gcttaggcat gggctccaga aagagaggca agttatggta gaggtcagaa
      421 agaattttac ttctttacct aactttcaaa gattggaggc aaaggtaaag gccagtcgag
      481 gccttgtgaa aagaaggttc gacttcatgc gtaacaccca tggacttaat ttctataaat
      541 caccagatgt taggaaactt gctcaagagt ctggaatgga tatttcagct tatgatgctt
      601 ttcttgcaga aaacgtccct tttatgactc ttattcagtg ctattgcgaa ggtgatgctc
      661 atgtaatgga accagtgatt actcctttga ttcctcaatt agaagggcaa tctcttgttc
      721 ttgatatgta tatattatct gcatacatcg actgtttgct tgctcatgca aaatctaaaa
      781 tgcaaaaggt atattgggtg gggacaggaa tagctgagta tattagaaac catcctccgg
      841 aaaaggatgc tattctaggg tctaaggaat ttcaaaaaca attgacagat gattttttta
      901 ccaagaaata atccaaggtt tcacttgcca cttgccaaac tcacgcagcg tatttaaaca
      961 aagaaaagct aatctctttt catgctaaca ggaatttttg gcagcagggg atctgtcaat
     1021 ttaagcctcc tctcacatgc tgtgagaaac cagccttcat tgatagtaga ctgcgcaaac
     1081 atcgcaaacc cgcatgtact ttttcctttt gcgcagcctg aggacatgca tgacatctat
     1141 gtggtgcagg ctgagcttat gtataagttt agggatatat taaaacacct ggacaggctt
     1201 gcaaagcagg taaatccaag gtgcatagta ataaccctct tcgacagact ttttcattat
     1261 aatgatgagc aggaaaacaa agacataata gaacatgcat gggagcttat gaaaaaacat
     1321 tcagaaagat atgagctaat agccggagca aatcatccct tagcaagcaa tttctgcgat
     1381 aacatgatcg gggtggaaga atgggacaca ctgtgcttag ccagaggata gttgctgact
     1441 tgatcataaa agagctgaat gaatacggga agtcacttag aggaaaagac agagagatct
     1501 tcaacaggct cttgaaagag cccctgaaac atatagggag catctcttat gcaaacagca
     1561 tgcatgcatg ggcttttttg ataatttcga tgcttattga aatggagaag aagaatggtt
     1621 aactaaatca tatagctata ctctctcttt tctcacggtt aatcctgtct accatattgc
     1681 tggtttcaaa caagctgtca aattcacttt gcacagtgtc acttaccata ttttcggttt
     1741 caaaccggtt gcccctgtta ctgaatgttt tatgttccat atttttcaac cttggcaggg
     1801 aaaaaatcca catacctctt gtaaaaaaaa tccaggcttc cctggtattc atccctgcct
     1861 aaaatgtcat catctaatct catgcttatc atctccttta cgttattgcc tcttcgttta
     1921 ggtaatcaaa cctaccccag ctatacagtt cacctgcgag taaccggtta tatccttttc
     1981 tgtattccat cttatcctcc ggtggttaat cactaggatt taaatccatt ttctttccca
     2041 ccatttcacc atattcacca tacacacatt cgttctcttc cattatccat cttccatttg
     2101 attgtatcac ttgccacctc cttattgacc actcgtcttt gtatctttag cgatattcct
     2161 gcttgccaat ccaggcatta tggcaaattc cccttttttc aagataagca agtcctacct
     2221 cgatatccca atcttctttc aacatcttaa ttacctcttg ctaacacacg attagggcaa
     2281 aatagcccta agctgctatt tgtcttttga gggcatacaa atgcttgaca agttttgtct
     2341 tgcctttttg ctagttccct taattgatct aaccacacgt tatatcgcag gaagtaacta
     2401 taactactta tggtagtaga aaacatctaa ggaacctaag gcaattgtta tctatccatc
     2461 ggcttgaatt tagctaaatt taaggttata ctaactttgt ttctatcggc attaaaaaca
     2521 ataatctctg ttactggaac taattaccaa ccttataata aaccagctga ttgctcaaga
     2581 cttgagtatg tactccgcag ctcggctagc ggtttgtgat ttttttatta aatcaggata
     2641 acttttttca cttgccactt gccatgcgca cacaggctat ataagaaata gaatcaactt
     2701 tttaggttaa gtgcctacac cccgagggga catccaggtt gggccttaat cacaatgaaa
     2761 gcttgggtca tggatgctta ccggaaagag aattcaatcg tcctttggct gaagactcct
     2821 gagaatgatt ttacagtcac tcggagtttt aaccactata tctatctaga tattgaatca
     2881 aaggaatttc ttgactcaaa gaagataaag tacaggggag ttaggaagaa ggattacatg
     2941 cgaagggaga tttctgtgct tgaggtaaag gttccgtggc tgtccaattt tgaggctttt
     3001 gtcaggtatc tagagaagga gacaaggcac agggttcctt tctataacgc tgacataacg
     3061 cctgagcaga tgttctttta caagaatgat tttttgccgt gcagcactgt ggaggtcgac
     3121 gaggggtcta taaggcctgt cctgggcgag actcctgtgc tttccaagat ggagcttaag
     3181 gtaaatgctg agaaggatat ctatttgaat ccagactgca aggttgtttc tataattgtt
     3241 gacagcgaga agatagaagg agatgaggtg catgtactaa gtacgtttaa gcagcggttt
     3301 ctttcaaagg accctgacat aatacacgct cagtttgctt tttcaaggat accttttctg
     3361 atgaggaggt ttctggcaaa cggcatagat tttagctttc atcgctttga tgatgtgccg
     3421 cttaagtata agggcgggag gactttcttt acatacggca tatcaagata ccgtgagttt
     3481 gctgtaaggc tgcacggcag atttctggta gacaactctt ctttcatggg aggagcgtgc
     3541 gaagcagatg cgataatgga gctttcgcaa ctgtctggga tgtttttgca gcagatagcg
     3601 tcaaaaagtt ttggcgctgc ttttcagttc aggctcataa aagagatggt aagccagaac
     3661 cttcttgtgc cgttcaagga aaaaccgata gagcagccgc tgtcgatgct tgagcttttg
     3721 aagacagaca gaggaggatt taccgctgat cccacagttg gctttcatga agatgtcgct
     3781 gagctagact tctgtagcat gtttccttgg ttgatatata accacaatgt ctctgctgac
     3841 accatacttt ctgatgaagg gccttttgag catgttcctg gaactccttt taagatatct
     3901 cttcggcaca aaggtcttgc agcagcaggg atcaaaaaat ttcttgacag gcggatggaa
     3961 tacaagcgaa atcctactgc ggtgaacaag gcaaaggcag ctgggcttaa gtgggtgctt
     4021 gtgtcaagct atggttatct tcgatttagg gagtttaagc ttggagtctc ttctgcacat
     4081 atgtcgatat gcgcctttgc aagagagata cttgtgaaac tctcaagggt tgctgaaagc
     4141 atgggctttg aggtggtgca cagcatcgtc gactctatct atataaagaa aaaagggata
     4201 actgaaaaag aggtcaagga attttgcaga gagatagagc tggagactgg gattcccatc
     4261 agcgtcgaag gaatctttaa gtggatagtt tttctgccaa gcataaatga taacgctcgg
     4321 cctgttccta caagatattt tggagtctat cggaatgggg agataaaggc aaggggaatc
     4381 gaggtaaggc aaagttcttg cccgttgata atcaaggagt tccaaaaaga atgccttagg
     4441 gttttgggga agtgctcagg caagatggag ataagggaga gtatacctga actttacaaa
     4501 ctcatggagg aatattcaaa gaaagtcggc aaagctcctg ctgcattgct tgttagtgcg
     4561 ctgagaatat ccaaggagga ctataagaat gatatccccc agcgaacgat tatcagttct
     4621 ttgaagaaaa aagggataac tatccgtgcc ggggaataca tcaattttgt gttttcaagt
     4681 cgagggccgg ttatccctga ggattattct ggaaatcctg acttttctca ctatagaaag
     4741 ctatttatca gatcgcttta ttccatcttg cagccgtttg gggtcgagag ggaaaagata
     4801 tcgtttaagt atgacaggca aaaaactttg agtgattata tgagcgtaaa aaatgtatat
     4861 attccgatgc tttcccagcc gcctgagaag cgcgggcttt ctgagaagat catcaagaag
     4921 aggctggtac ttcaaggttg gactgtatgg aaaggagggc ttcttggtat agagaaagtt
     4981 tcagagtatc ctaattttat caggaagtat attctgctga agcagctgct ttcaaaagtt
     5041 gatgagaagc tgcctgaaga gcttgagtat atgtccaacg tgcaccacgg catgcctgac
     5101 tatatctgct atcggaatgg caagttcaag tttgtcgagt gcaaattaca gtatgagctt
     5161 ctttctgaca agcagaagaa gtgcctgatg aaacttaaag ggatgggatt tgacgtcgaa
     5221 gtgcatcgat tggtcgatag aaggacaaag gcaagagagg cgatggtgga tgtggtgaat
     5281 gggggaaaaa tgattatgga ggtgcagaat aggcttgtta caccccatta gtagttaatt
     5341 atcaaaaggt ttttaaagaa tgagaggact ccttaaatca tggctaaaga agactcaact
     5401 ccaatacctc taacaaaaaa agacatcgcc aatctattga gtggaaaagc gtttgaaaga
     5461 ctgctggtta aatcatcaag atatactgcc cgtactggtc agacaagcgg attttatgca
     5521 acaagggata ttttggggca atgtcatttt tctaaagtcg atactgaggg ttatgcatca
     5581 aatagccttt caataaaggt aaatgatgag atgggaaaat atccattgat gcaccttcag
     5641 ttctgtacaa ataatgggta tatctcccct ccaggtagag gtgacctgtt agcatatact
     5701 ctattaaaaa accaaggggc ttatcagtcc aggccgataa atatacttgc acaggtagat
     5761 gatttgggca acggagagct tgtcttgctg caggaaaaag atgttgagac attgtctgct
     5821 gcagatttga gcattatctg ccaaaaaacc aggcatctaa tacaaggtga acattcagct
     5881 gaagaaatag caaaagcgtt ccaaaacacc ggatattata cctctacaac catgcggttt
     5941 caattacata aaagaaacgc tttgatccca gaaccggaca ttaggcgagc atgcaagttt
     6001 gcctacactc cagttatgag gccaggttaa aacctattgt aaacagccac caatgtatta
     6061 tccacaacaa cttctttttc cttaactaaa ttagatggat ggaaatgatg cccaaacaag
     6121 atcagcttgc cgctcaattc gttcaagagc tttttttcaa ggcccctttg aagaggcttg
     6181 tcagggttga taaagacgat atcatatcct tctatcgagt gctcgttaaa atcatcatgg
     6241 tgaaagacta cgtttgatat tcccaactta tcttttatct caagggcttt gtgatggagc
     6301 tcgtcgtcta tctctattcc ttctgcacga ctgcaaaata aagaagcaag aagagtcact
     6361 tttccatcac ctgagccaag gtcgataaaa ctgcttttct tgctaagctt taacttcctg
     6421 aatgcttcaa agacttctcc agttatcgca gcgttccaga agccaagctc tgtcgatctt
     6481 aatgggagct ttccattcct aagcagctct ttgtagtaat catcataagc tgcctttatc
     6541 tgcgtaaact tttgctctct catatgtttg tgtgatattg ccttatcttt cttacgatag
     6601 accttgtggc aacaacaagg tctgccactt ccttgcttgc aacacgatag cttatctttt
     6661 cgaccttttc gccaagcttg tctatccatt caagctgcct gttgctcagg ggctcttcgc
     6721 tttcatagag tccttttgct gcttcataaa catgggcaag aagatagagt tgggatgagc
     6781 tctcctctgc aggagctgat ttttttaccg tgccttcaag accaggctcg accttaaaaa
     6841 gactctcgat gtctatgggc tttgtctctg gaactttcac tgactctgca atgccctgct
     6901 tttccagctc tgcctgggtc tgctttatca gcttttcagc ttcttctatc tcttttttac
     6961 tttcttcttc aagctctttg agcttcttta tcttttctgc aggcttaagc ttgtcaagct
     7021 cttctttatt tatcttgggc atagtatcat tccatcggaa cctggctcca tagtgtccag
     7081 tcattgcttg gagcatatgc accaaatgta aacatgggcg gctgggactg gccaaaatat
     7141 gagtaatgga cgtctgacca cgattgaggt atccttgcca tgcctcctgc tcctgtcccg
     7201 tagattggag agccaaggta tctccaggtc ttcatgaggc cgtggaaatc tgagagatca
     7261 gtgcttgcgt ctgctccagg ctctacggta atcgctttgt catatttata ttttttcaag
     7321 atatccatga cttccctgat gggagcgttt ccctggcctg gcgcaacgtg atcatcatga
     7381 tagccaaagt tgtctgtaag gtggatgttt ccgacatagc cgttcttgac aagcttttca
     7441 aactcttttg tataccactg cttgaactct ttgtcagcgt cttctatcgt ctgaccaggc
     7501 tttatctgcc agtattttct ccatgtattt gcatggccag tgtcaaatgt tgctttgata
     7561 tatttttcag cgagcttttc tgcttcttcc ttattcaggc ttggctgata gtaagggctt
     7621 atgccgtctt tgttgtttgg ataatcccct ttttcagtga ttccaaattt gaccctttct
     7681 ttagtgagga aatcgacaat cc
//
LOCUS       DASRUK010000184        14010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2000582, whole genome shotgun sequence.
ACCESSION   DASRUK010000184 DASRUK010000000
VERSION     DASRUK010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 14010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14010
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2000582"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..594)
                     /locus_tag="VFF28_07910"
     CDS             complement(88..594)
                     /locus_tag="VFF28_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45579.1"
                     /translation="MDEKNQNQFDLFKLHDRVQDKDPRWSWIRASGKDLAPYVYLLIR
                     ELTEKYGTNNTAKEIKKLVPKLSIDSILNYFWSKYESFPLVFLEAISMQFTNQTKISQ
                     EIQKRIDILSISRYDSKPIKAIKELNLSLCKLAGAHAADGALWGESNYQLTSTPTLKG
                     GDSVELLA"
     gene            complement(673..1299)
                     /locus_tag="VFF28_07915"
     CDS             complement(673..1299)
                     /locus_tag="VFF28_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018154396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZX45580.1"
                     /translation="MANLSFLKTPPTKIICVGLNYHCHAKELGMNTPHHPIIFLKPLT
                     SLIGPKDNIVYPRQSKRVDYEGELGIVIKKQGKGIQKEKAMQYVGGFTCINDITARDL
                     QQLDGQWTRAKSFDTFCAVGPKIASLKDPNNVKIQTLLNGEIKQDSSTKEFIFKVEEI
                     IAFISSVMTLNPGDIIATGTPKGIGPMKKGDTVEIRIEGIGNLVNTVT"
     gene            1463..2203
                     /locus_tag="VFF28_07920"
     CDS             1463..2203
                     /locus_tag="VFF28_07920"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45581.1"
                     /translation="MVGIYDKDLLSYGTVKVGFFGGAGEPGLCGERGRGVSSASLDAS
                     RALGAYIPRFGASGNKIIQEISGSPPGNIEALTRAAYKSGSRAIGISPFYDEAHHLRE
                     GSPTKGYSVIVYVGSDKKASLDTYEGRRRLNKQFSFRDNLNLQAAHCAIILGGKDGTD
                     EERAIAKMRGMSIGLVASTGGVAEKSAGYMRDICGDTGSIIITDRDPIAVFDQLLALE
                     LQKSGEHERTIIDDIIEKTNDIKLELKR"
     gene            complement(2220..3221)
                     /gene="ilvC"
                     /locus_tag="VFF28_07925"
     CDS             complement(2220..3221)
                     /gene="ilvC"
                     /locus_tag="VFF28_07925"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014405165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HZX45582.1"
                     /translation="MINILTDKDADESFLEGKTVAIIGYGSQGSAQANCLKDSGVSVI
                     IGETENLGGKANPSWIRAKQEGFEVYEIDKAAEKAEIIHILVPDEFQADVYNASVKQF
                     MRAGKTLCFSHGFNICYKRITPPENVDVIMVAPKAPGPEERRQYLEGFGVPGLIAIHQ
                     NPSGTARDIALAMAKAMHWTRAGCLECTFEQETYEDLFGEQAVLCGGATELVKAGFDT
                     LVEAGYPPEMAYFECLHELKLIVDLMYKGGFTEMWKSVSNTAKYGGLTRGKEIITDKT
                     RKVMKKMLKDIESGKFAREWVDECAKGKPLLNKLQEEDFNHPIEKVGKEIRELFRKK"
     gene            complement(3261..4400)
                     /locus_tag="VFF28_07930"
     CDS             complement(3261..4400)
                     /locus_tag="VFF28_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HZX45583.1"
                     /translation="MKTYKIAKLPGDGIGPEIISEGVKVLDAVADKNNFKIDWEYYDN
                     GADKYLATGELITEETLKEIKQKCKAIYLGALGDPRCKPGILEKGILLKTRFYFDQYV
                     NLRPIKLLEGVSCPLKDKTPKDINFTVIRENTEDFYVGIGNRAKEGNSKQQLEVIRSL
                     YQVKFNLDIEAQKSEIAYQIGVMSKKGAQRVIKYGFELAKKEKAEKAYFVDKANVLSD
                     IYGFWRENVEEIAKSYPKIPYEFMFVDAITMWFVKNPEWFKVVTTPNMFGDIITDLGA
                     MIQGGLGLAPGGNINPEGISMFEPIHGSAPKYKGMNTANPIATIWAGALMLDELGEKK
                     ASSQVVDAIQSVLKEGKVRTKDIGGTAKCTDMGDAVKKALDGQSL"
     gene            4487..5068
                     /locus_tag="VFF28_07935"
     CDS             4487..5068
                     /locus_tag="VFF28_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45584.1"
                     /translation="MCRIAVVGPDGSGKSTLLEKIASALPDLKVVRMPELRHRNGISE
                     NAAALQAIVTLTPYLIRTFLRGGGITLFERCAAIDFQVYGKVYGKIGRLLGEYISSVA
                     PLPSHVLYLDIDIATALSRISKDKHQHHETPERMGIIIERYQGVLNRLVEKSLIELHE
                     INARAEPDKVYHDAMNIIGPLYEHSKKCQRKIC"
     gene            5226..5900
                     /locus_tag="VFF28_07940"
     CDS             5226..5900
                     /locus_tag="VFF28_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45585.1"
                     /translation="MGIGMSTPPGIGAPISMTCRPLWTLANRLGDYLRGKPESIHNLE
                     VMLLSAIPTVGNFAYLANIVENPELFGFFTYHISQNLQNKKVKGVVDYIANQREKILK
                     GLSAIHANYEAPLALASAYGFASENTPLAIFAMAATAAILYKEYKEGKPSALPRAMRI
                     LPVVGALLIARNKEVVDLSIRTVESLSTLALVYIGYTLKAIVLEYKYRLGDQKGYTNL
                     RDVLPF"
     gene            complement(5917..6399)
                     /locus_tag="VFF28_07945"
     CDS             complement(5917..6399)
                     /locus_tag="VFF28_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HZX45586.1"
                     /translation="MSTLKGTAHVYNKDHINTDEIIPARYLVSDKEEELAKHAMEDLD
                     KDFVNKVKPGDFIVAGENFGCGSSREHAVWSLRGSGVKAVIADSFARIFFRNCINNGF
                     YAIECKGASKELKDGEQLEIDIKKGMIKTSRKELKFAPFPDFAVELINAGGLFNYIKK
                     "
     gene            complement(6396..7658)
                     /gene="leuC"
                     /locus_tag="VFF28_07950"
     CDS             complement(6396..7658)
                     /gene="leuC"
                     /locus_tag="VFF28_07950"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HZX45587.1"
                     /translation="MPTITEKILAAHCGRKEVKPGELINADIDIIMCHDVTTPPAIEM
                     LKKRGITKVFNPDKVVVTPDHFVPNKDIASAELAKRLRQWVSSCNIKNYYEVGKHGVC
                     HAILPEQGHILPGELIIGADSHTCTYGALGAFSTGVGSTDLAAALATGKLWFKVPESI
                     KFVINGKLPKGVYSKDIILKVISDIGVDGALYKAMEFTGDGINSLSVEARMTITNMAI
                     EAGAKSGIIEADQKVVDYVKQRTKKKFAIIKSDEDAVYSEVKEYDLTDLEPMIAFPDL
                     PSNGKLISQVREIKVDQAYLGSCTNGRIEDLRIAAQIMKGNKVAKGTRMLVVPATTDI
                     WKMANKEGLIDIFMESGAAVSTPTCGACLGGHMGILAAGERCISSTNRNFTGRMGSPK
                     SEVYLASPATVAASAIEGKIADPRRYIK"
     gene            7716..8444
                     /locus_tag="VFF28_07955"
     CDS             7716..8444
                     /locus_tag="VFF28_07955"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZX45588.1"
                     /translation="MEAELIKAGLSQNEARIYLSLIKKGPSPASALTKETTVSRPHIY
                     DSINKLIEKGLVSYVIKDSRRYFKAADPKELINFLEDEKEKINAQQEEIKKVLPNLSG
                     LRGQKPSGTSVEVYEGKDGLKTVLMDVCNYSKDFIAFGATYNFEEVLPLFSKLFIQRR
                     ERKNQRAKILVVQGEKPVKTKLNTYRWLPKEYSLPNSSIIYGDKTAILLWTGEPLGII
                     IKNKEVAKSYQSYFELLWKIGKNK"
     gene            complement(8447..8935)
                     /gene="ilvN"
                     /locus_tag="VFF28_07960"
     CDS             complement(8447..8935)
                     /gene="ilvN"
                     /locus_tag="VFF28_07960"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HZX45589.1"
                     /translation="MAKEQSHVISMLVEDQPGVLTRIAGMFARRGFNIDTITVGKTSK
                     PGVSKMVITVIGDETVLEQVEKQVNKLIDTIKVTEMPPDKSVIRELCLIKVAIPNKKA
                     KEEILRYTKIYKTKIMDITSKSVTVELIGLPDKIDAFIELMKEFGIKEISRTGVTAVS
                     RG"
     gene            complement(8937..10652)
                     /gene="ilvB"
                     /locus_tag="VFF28_07965"
     CDS             complement(8937..10652)
                     /gene="ilvB"
                     /locus_tag="VFF28_07965"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012901446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HZX45590.1"
                     /translation="MNGAHILIETLIKNGVDVTFGFPGGQVIPLFDAFLDYKGKIKNI
                     LVRHEQGAAHAAEGYARASGKAGVCIATSGPGACNLVTGIADAYMDSVPVIALGGQVP
                     THLIGNDAFQETDMMGITIPITKHNYQVRTPDQMAPIVMKSFKIATNGRPGPVYIDIP
                     KDTQAGQATESIPIEVTIPGFKPTLAPNPIQIKKAAEAIIAAEKPLFLIGQGVIISNA
                     SKELEELINLSKIPVATTFMAKGAFNEYHPLSLGTVGMHGKKIANYATLKTDLLISIG
                     CRFSDRITGNLKTYAENAKVIHSDIDPAEIGKNVEVDIPIVGDAKQIIIALTQEIKKM
                     AIKEKTAWTKRIKECKAICDECIPIPQTKKIHPKTIMLTLNKILAENDIVVTGVGQHQ
                     MFAGHFLKFRKPRTFISSGGAGTMGFGLPAAIGAKVAKPDSNIFLIDGDGSFQMTIQE
                     LGTIKESGIKIIVIIMNNSYLGMVRQWLELFSDKRYSEVYLGTTPDFVKVAEAYGLKG
                     IRVEKGQDLEPALKQAIKNDLTTIIDVVTEEEANILPMLSPGGNIKDAFGGCMQAPGK
                     FFKGD"
     gene            complement(10661..12226)
                     /locus_tag="VFF28_07970"
     CDS             complement(10661..12226)
                     /locus_tag="VFF28_07970"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HZX45591.1"
                     /translation="MKKIIIFDTTLRDGEQSPGASLTTPEKLIIAKQLAKLGVDVIEA
                     GFPISSDGDFEAVKLIAQEVKGPTICGLARTKKEDIDRSWEAVKYSKKPRIHTFIATS
                     DIHIKEKLRKTKEEVIKMAVDAVKHAKSLCDDIEFSPEDSARTDINYMCDIVKATIEA
                     GATTINIPDTVGYAEPEEFGHRIKTVFDKLGDVIKKNKVIISVHCHNDLGLAVANSLA
                     AVKNGATQVECTINGIGERAGNCSLEEVVMNLKTRNDFFSEVETTINTKQIFKASQLV
                     SQLTGLAVQRNKAIVGANAFAHEAGIHQHGVMNSRSTYEIMQPEQIGWIGTNLVIGKH
                     SGHHAIEQALKEAGFDLAKDKVQKVIEKVKELADKQKEVMREDITAIAADIAAGLSKE
                     EEIIKLEEVKVTSGNKLQPEAEVTVTIDNQTKTGKGKGVGPVDALSHAIRTLIDPSII
                     LKEYGLEAITGGTDALANVSIKVQDPNGNIFKSEAVNEDIIMASAIALVKGMNKALNF
                     QRKNMKQRIHAQY"
     gene            12830..13456
                     /locus_tag="VFF28_07975"
     CDS             12830..13456
                     /locus_tag="VFF28_07975"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HZX45592.1"
                     /translation="MLITITRLLITFLLSFAFGYERQRSHKTVGIGTFIFVALGACIV
                     GIIAESIWELNPIGLLASVVTGIGFLGAGALIKGSDKVFGFTTASAVWLFSIFGLTVG
                     IGKYEIALATYVLVWIVILVDKHFEQHGIGSYQRRLIITSNKMIEEKEINRHLVMGTK
                     KYKLLGVELRKQVNEFQMTYLVEGTQESLNKLVKSLYKEEWLKEAKVE"
     gene            complement(13457..13738)
                     /locus_tag="VFF28_07980"
     CDS             complement(13457..13738)
                     /locus_tag="VFF28_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45593.1"
                     /translation="MLSQASLDRLKAKQLELRHLKLKHYQEDFDKYIASLFKDEQCQY
                     MTGISGIISLCNNNLECKFKGEVYKSFTAEPKRECSRERALRFEKMLEH"
BASE COUNT         3624 a         2985 c         2780 g         4621 t
ORIGIN      
        1 cctttggggt gatgggaagt ttatggcttc tgttggccat atcaccttag aaaaagccaa
       61 agaatatcta gagaagcaag agactcatca tgccaataat tcgacggaat ccccaccctt
      121 cagggttggg gtggaggtca actgataatt ggattcaccc cataaagcac catctgctgc
      181 atgtgctcct gcaagcttac aaagcgatag atttagctct tttattgctt tgattggctt
      241 tgaatcatat cttgatatcg aaagaatgtc tattcttttc tgtatctcct gactaatctt
      301 ggtttgatta gtaaattgca ttgagatagc ctcaagaaag actaatggaa agctttcata
      361 ttttgaccaa aagtaattga gtattgaatc aatggataac ttgggaacca atttctttat
      421 ttccttggca gtattgttag tgccatactt ctctgttagc tccctgatca gaagataaac
      481 ataaggtgct aaatcttttc ctgaagccct aatccaactc catcttggat ccttatcttg
      541 tactctatcg tgcagcttga aaaggtcaaa ctgattttga tttttttcat ccattctaaa
      601 ttatccaata aaagcaagtt aaatatcttg cgtcaccatt tggcgagtgg caagtgtata
      661 ctataaagaa agctaagtaa ctgtatttac taagttgcca ataccttcaa tcctaatttc
      721 aacagtatcc cctttcttca tagggccaat acctttgggg gttcctgtag caataatgtc
      781 tccgggattt aatgtcatta cgcttgagat aaaggcaatt atctcttcca ctttgaagat
      841 aaactctttt gtgcttgaat cctgctttat ctctccatta agaagtgtct ggatcttaac
      901 attgtttgga tcttttaggc ttgctatctt tgggccgaca gcgcaaaaag tgtcaaagga
      961 ttttgccctt gtccattggc catcaagctg ctgaaggtct cttgcagtta tatcgtttat
     1021 gcaggtaaat cctccaacat attgcattgc tttttctttt tggatgcctt taccttgctt
     1081 ttttatgact atacccagct cgccttcata gtcaactctc ttactttgcc tcggatagac
     1141 aatattatct tttgggccta tcaatgatgt taagggcttt aagaagatga taggatgatg
     1201 gggggtgttc atgccaagct cttttgcgtg gcaatggtaa ttgaggccaa cacagataat
     1261 tttagtcggt ggtgtcttga gaaacgatag gtttgccatg atattttcta gctggctacc
     1321 tctataaaaa tcttgttaaa gatagtattt atataaaatt atttggtgat tgcttggcag
     1381 gtgatcatta atcggatacg ccatctatac atttgactac catttgcaaa ttggcgatga
     1441 cagaaaaagc atcatccctt gcttggtagg gatatacgac aaagatctcc tttcctatgg
     1501 cacagtaaag gtcggattct ttggaggagc tggagagcct ggactttgcg gggaaagggg
     1561 cagaggagta agcagtgcaa gccttgatgc ttcgagagcg cttggagcat atatccctag
     1621 atttggagca agcggaaata aaataataca ggagataagc gggtctccgc caggaaatat
     1681 agaggctttg acaagagcag cctataaatc aggttcaaga gcgataggca tatcgccttt
     1741 ctatgacgag gctcatcatc taagagaggg ctctccgaca aaagggtata gtgtgatagt
     1801 gtatgtcgga tctgataaaa aagccagcct tgacacatat gaaggaagaa gaaggctaaa
     1861 taaacaattt tcttttcgag acaatcttaa tctacaggca gcgcactgcg caatcatcct
     1921 aggtggaaag gatggcactg atgaagagcg ggcaattgcg aaaatgagag gaatgagcat
     1981 agggcttgtg gcttcaacag gaggggttgc tgaaaaaagc gcaggataca tgagagatat
     2041 ctgcggggat actggctcta taatcatcac agatagggat ccaatagcag tgtttgacca
     2101 gctactggct cttgaacttc agaagagcgg tgagcatgaa agaacaatca tagatgacat
     2161 aatagaaaaa actaatgata taaagttgga actgaaaaga taaataaaaa gataaaaaat
     2221 tatttctttc taaacaattc cctaatctcc tttcctacct tttcgatagg atgattaaaa
     2281 tcttcttcct gaagcttgtt tagaaggggc tttccttttg cacactcatc tacccattct
     2341 cttgcaaact ttcctgactc gatatctttg agcatcttct tcatcacttt tcttgtcttg
     2401 tctgtgatta tctcttttcc tcttgtaagg ccgccatatt ttgcggtgtt tgagacgctt
     2461 ttccacatct ctgtaaagcc gcctttatac ataaggtcta caataagctt tagctcatgg
     2521 aggcattcaa aataagccat ctctggagga tatcctgctt caacaagggt gtcaaatcct
     2581 gctttgacca gctctgttgc tccaccgcaa aggactgcct gctctccaaa caagtcttca
     2641 taagtctcct gctcaaaagt gcactcaagg catcctgctc ttgtccagtg catagctttt
     2701 gccatggcaa gtgcaatgtc tcttgcagtg ccgcttggat tctgatggat agcaataagg
     2761 cccggtacgc caaagccctc cagatattgc cttctttcct ctgggccagg cgcttttgga
     2821 gcaaccataa taacatcaac attttcagga ggggttatcc tcttataaca gatattaaat
     2881 ccgtgggaaa aacaaagagt ttttcctgcc ctcataaatt gctttacact tgcattatag
     2941 acatctgcct ggaattcatc aggcacaaga atatggatga tttctgcttt ttctgcagct
     3001 ttatctatct cataaacctc aaagccttcc tgctttgccc taatccagct cgggtttgcc
     3061 tttccgccaa ggttttctgt ctctcctatt atcacgctaa ctcctgaatc cttcaagcag
     3121 tttgcctggg cacttccctg gctgccataa cctataattg ccactgtctt tccttctaaa
     3181 aaggattcgt ctgcatcctt atctgtcagt atgtttatca ttttatcatt cctcctctgt
     3241 atatgtaagt ggtaaataat ttataaagat tgcccatcaa gggctttttt tactgcatct
     3301 cccatgtcag tgcactttgc agttcctcca atgtcctttg tccttacttt tccttctttt
     3361 agtactgatt ggatagcatc aactacctgc gaggatgctt ttttttctcc taactcatcg
     3421 agcataagcg ctcctgccca gatggttgca ataggatttg ccgtgttcat ccccttgtat
     3481 tttggcgctg aaccatgtat cggctcaaac attgagatgc cttctgggtt gatgtttcct
     3541 cctggtgcaa ggccaagccc gccctggatc atcgctccca aatccgtgat gatatctccg
     3601 aacatgttgg gggttgtcac caccttgaac cactccgggt tttttacaaa ccacatggtt
     3661 attgcatcta caaacataaa ttcatagggt atctttggat agctttttgc tatctcttca
     3721 acattttctc tccaaaagcc gtagatatct gaaaggacgt ttgccttatc tacaaaatat
     3781 gctttctctg ctttttcctt ctttgcaagc tcaaagccat attttatcac tctttgcgct
     3841 ccctttttgc tcatcacacc tatctgatat gctatctcgc ttttttgggc ttctatgtcc
     3901 aggttgaact ttacttgata taatgatctt ataacttcca gctgctgctt tgagtttcct
     3961 tcttttgccc tgtttcctat ccctacatag aagtcttcgg tgttttccct tatgactgtg
     4021 aaatttatgt cttttggagt tttgtcttta agcggacagg aaactccttc caaaagcttt
     4081 ataggcctta ggtttacata ctggtcaaag tagaacctgg tctttaacag gattcctttc
     4141 tccaggattc ctggcttgca ccttgggtcg ccgagtgctc caagatatat tgccttgcat
     4201 ttctgcttta tctcctttaa agtttcctct gtaatcaatt ctcctgtcgc tagatacttg
     4261 tcagcgccat tgtcataata ctcccaatct atcttaaagt tatttttgtc agcgactgcg
     4321 tctagtacct tcactccttc tgatattatc tcaggcccga tgccatctcc tggcagtttt
     4381 gcgattttgt aggttttcat tttattgagt gaaaattggg agatatttaa atatcttttt
     4441 aaacaactaa ttattccact aaggaaaaga ggttcttgga gcattcatgt gtagaatagc
     4501 agttgtagga ccagatggct ctggcaagag cactttacta gaaaaaatag ctagtgcatt
     4561 acctgatctt aaagtagtaa gaatgcctga actgagacat agaaatggaa ttagcgagaa
     4621 tgctgctgcc ctgcaagcta ttgtaacgtt aactccttat ctaatcagaa cttttttaag
     4681 aggggggggt ataactttat ttgaaagatg tgcagcgatt gatttccagg tttatggtaa
     4741 agtatacggt aaaattggta gattgcttgg agaatatatt tcatctgttg caccactacc
     4801 cagccatgta ttatatttag atatagacat tgcaacagct ttaagcagga ttagtaagga
     4861 taagcaccaa catcatgaga caccggaaag gatggggatt ataatagaaa gatatcaagg
     4921 tgtcttaaac agacttgttg aaaaaagcct gattgaattg catgaaatta atgctcgtgc
     4981 agagccggat aaggtttacc atgatgcaat gaatattata gggccgcttt atgaacactc
     5041 gaaaaaatgt caaagaaaga tttgttgagt ggattaatca ccaaagatac cgttgctcgg
     5101 aacgaggttt tagggataca ttagatgatc tcgttagtca acagagggta agtatagaag
     5161 atgctcccag attactacaa caggcgctag ccaatagata tttgatacat catctgtcta
     5221 tccatatggg cataggaatg tctacaccac ctggaattgg ggcgccaata tccatgactt
     5281 gcagaccctt atggacacta gctaacagat taggtgatta cctaagagga aagcctgaat
     5341 cgatccataa cctagaggtc atgctgcttt ctgcgatacc tactgttgga aactttgcgt
     5401 atcttgccaa tatagttgaa aatcctgagc tttttggttt ttttacttat catataagcc
     5461 aaaacttaca gaataaaaag gtaaaaggag tcgttgatta catcgcaaac caaagagaaa
     5521 aaattctaaa aggcttaagc gccatacatg caaactatga agccccattg gcgttagcaa
     5581 gcgcttatgg atttgcaagt gaaaatactc ccctagcaat ttttgctatg gccgctactg
     5641 cggcaatact ttataaggag tataaggagg gaaagccttc agcattaccc agggcaatga
     5701 gaatacttcc tgtagtggga gcattattaa tagccagaaa taaagaggtt gtagatttaa
     5761 gcattagaac tgtggaatcc ctgtcaactc ttgctttggt gtatattgga tataccctaa
     5821 aggccatagt actagaatat aaatataggt tgggtgacca aaaaggatat acaaatttaa
     5881 gagacgtatt gcctttttga tgttgctatg tcgcgattat ttcttaatat aattaaataa
     5941 tcctcctgcg tttatcagct caacagcgaa atccgggaac ggtgcaaact tcagctcttt
     6001 tctgctggtt tttatcatgc ctttttttat gtcaatctcc aattgctcgc catcctttag
     6061 ctcctttgat gcgcctttgc actctatcgc ataaaaacca ttgtttatgc agttcctaaa
     6121 aaaaatccga gcgaaactgt ctgcgataac tgcttttact cctgagcctc taagtgacca
     6181 tacagcatgc tctctgctag agccgcagcc aaagttttcg cctgcaacta tgaaatcgcc
     6241 tggctttact ttgtttacaa agtctttgtc cagatcttcc atagcatgct ttgcaagctc
     6301 ttcttctttg tctgaaacaa gatatcttgc cgggattatc tcatctgtgt tgatgtggtc
     6361 tttattgtag acgtgggctg ttccttttag tgtgctcatt ttatgtacct tcttggatct
     6421 gcaatctttc cttctattgc tgatgctgcg actgttgccg gggaagctag atagacttct
     6481 gactttgggc tgcccatcct tcctgtaaag ttcctattgg ttgaggaaat gcatctttcg
     6541 cctgcagcta atatccccat gtggcctcca aggcatgctc cgcatgtcgg ggttgagact
     6601 gctgctcctg attccataaa tatgtcgatc aatccttctt tatttgccat cttccatatg
     6661 tcggttgtcg ctggaacaac aagcatcctt gtgccttttg caactttgtt tcctttcatt
     6721 atctgtgcag ctatccttaa gtcttcaatc cttccgtttg tgcagctgcc taggtaagcc
     6781 tgatcaactt ttatttccct aacctgagag ataagtttcc catttgaggg caaatcagga
     6841 aaagctatca tcggctctaa gtctgttaaa tcgtattctt ttacttctga atatactgca
     6901 tcttcatcgc tcttgataat tgcgaatttt ttcttagtcc tttgtttaac ataatccacg
     6961 actttttggt ctgcttcaat tatccctgac tttgcgcctg cttctattgc catgttggtt
     7021 attgtcatcc ttgcttccac agacaggctg tttattccat ctcctgtaaa ttccatggct
     7081 ttgtataaag cgccatctac tccgatgtct gagataactt ttaggattat atctttggag
     7141 taaactcctt ttggcagctt gccgttgata acaaatttga tagactcagg gactttgaac
     7201 catagctttc ctgttgccag agctgctgca aggtctgttg agccaacgcc tgttgagaag
     7261 gctccaaggg caccataagt gcaagtatga gaatctgctc cgataatcaa ttctccaggc
     7321 aatatgtggc cttgctctgg caatatcgca tggcatactc catgttttcc aacctcatag
     7381 taattcttta tattacaaga gctaacccac tgccttaatc tttttgccag ctctgctgag
     7441 gctatgtctt tgtttggaac aaaatgatca ggagtaacaa cgactttgtc agggttgaag
     7501 accttggtta ttccccgctt ttttaacatc tctatagcag gaggggtggt cacatcatgg
     7561 cacatgatta tatctatgtc tgcgttgata agttctcctg gctttacttc ctttctgcca
     7621 cagtgagcgg ctagtatctt ttctgttatg gttggcatgg tatgctatga aaattaagct
     7681 gtatttaaat atatgttgtt cttttttagt acaacatgga agcagagctg ataaaagcag
     7741 gccttagcca gaacgaggca agaatctatc tgtccttgat aaaaaagggg ccatctcctg
     7801 caagcgcact cacaaaagag acaactgtct caaggccgca tatctatgac tctataaaca
     7861 aattgatcga gaaggggctt gtgtcttatg taatcaagga cagcaggagg tattttaagg
     7921 cagctgaccc caaggagctg atcaattttc tggaggatga gaaagaaaag ataaatgccc
     7981 agcaggaaga gataaaaaag gtactgccaa atctttccgg ccttagaggg cagaaacctt
     8041 ccggcacctc tgttgaagtc tatgaaggaa aagacgggct taaaacagtt ctcatggacg
     8101 tatgcaacta cagcaaagac ttcatcgctt ttggagctac atataatttt gaggaagttc
     8161 ttccactatt ttccaagtta ttcatccaaa gaagggaaag gaaaaaccaa agggcaaaaa
     8221 tccttgttgt gcaaggggaa aagcctgtaa aaaccaagct taacacttac agatggctgc
     8281 ctaaagagta ttcgcttccc aattcatcga taatatatgg agacaagaca gcaatacttc
     8341 tttggacagg tgagcctctt ggaataataa tcaagaataa agaagtcgct aagtcgtatc
     8401 aaagctattt tgaactgttg tggaagattg gaaaaaataa gtaagtttat ccccttgaaa
     8461 ctgcggtcac tcctgtcctt gagatctctt ttatcccaaa ctctttcatc aattcgataa
     8521 aggcgtcaat cttgtcagga aggccgataa gctccactgt tactgatttt gaagttatgt
     8581 ccattatctt tgtcttgtag atcttcgtat accttagtat ctcttccttt gctttcttgt
     8641 ttggaatagc taccttgata aggcaaagct ccctgataac tgatttatcc ggaggcatct
     8701 ctgtcacctt gatagtgtca atcagcttat ttacctgttt ttctacctgc tctaagactg
     8761 tttcatctcc tataactgtg atgaccatct tgcttacgcc tggctttgag gtctttccaa
     8821 ctgtaatcgt atctatgtta aaaccccttc gtgcaaacat gcctgcaatc ctagtaagca
     8881 caccaggttg atcttcaaca agcatcgaaa taacatgact ttgttctttt gccatatcaa
     8941 tcacctttga aaaatttacc tggtgcttgc atacaaccgc caaaggcatc ctttatgttt
     9001 ccacctggcg agagcatagg taagatattt gcttcttctt ctgtgacgac atctatgatc
     9061 gttgtaaggt cattttttat cgcctgcttt agtgctggct caagatcctg gcctttttca
     9121 acccttatac cttttaggcc ataggcttct gcaaccttga caaaatcagg ggttgttcct
     9181 aagtatacct ctgaatatcg cttatctgaa aacagctcaa gccactgtct gaccataccc
     9241 agataagagt tgttcatgat tataactatt atctttatac ctgattcctt tatagtcccc
     9301 agctcttgta tcgtcatctg aaagctgcca tcgccatcta tcaaaaatat atttgagtct
     9361 ggctttgcaa cttttgcacc tatggctgct ggaaggccaa agcccattgt acctgcaccg
     9421 cctgagctta tgaatgttct tggctttcta aatttcagaa aatggcctgc aaacatctgg
     9481 tgctggccga cgcctgtgac aacgatatca ttttctgcca atatcttgtt taaggtaagc
     9541 ataatcgtct ttggatgtat cttttttgtc tggggaatag ggatacattc atcgcatatt
     9601 gccttgcatt cctttatcct ctttgtccag gctgtctttt cttttattgc catcttcttg
     9661 atttcctgcg tgagtgcaat gattatctgt tttgcatctc cgactatagg gatgtcaacc
     9721 tcaacatttt ttcctatctc cgcagggtca atatctgaat ggataacttt agcattctct
     9781 gcatatgtct ttaagttgcc tgtaatccgg tctgagaacc tgcagcctat agagatgagt
     9841 aggtcagttt ttaaggtagc atagttggct atctttttcc catgcatgcc cactgttcca
     9901 aggcttaggg gatgatattc attgaacgcc ccttttgcca tgaatgttgt tgcaaccgga
     9961 atctttgaaa gatttataag ttcttcaagt tcttttgaag cgttggaaat gatgactccc
    10021 tggccgatga ggaacaaggg tttttctgct gctattatcg cctctgctgc cttctttatc
    10081 tggatcggat ttggagcaag tgttggctta aatccaggga ttgtcacttc aatgggtata
    10141 gactctgtag cctgccctgc ctgggtatct tttggaatgt caatatagac agggcctggc
    10201 ctgccgtttg tcgctatctt aaatgatttc atcacaatag gtgccatctg atcaggagtc
    10261 cttacctggt aattgtgctt tgtgattgga atcgttatgc ccatcatgtc tgtttcctgg
    10321 aatgcatcat ttccaatcag atgagttggc acctggcctc ctagagctat gaccggaaca
    10381 gagtccatgt atgcgtcggc aatgcctgtg accagattgc atgctcctgg accagatgtc
    10441 gctatgcaaa ctcctgcttt tccagaggct cttgcgtagc cctctgctgc atgggctgct
    10501 ccctgctcgt gcctgacaag gatgtttttt atctttccct tatagtccag gaaagcgtca
    10561 aataatggga tgacttgtcc gcccggaaag ccgaaagtta catctacacc attttttata
    10621 agggtttcaa tcaatatatg tgctccgttc atagtatcac ctaatactgc gcatgtatcc
    10681 tttgcttcat gttctttctt tggaaattga gggctttgtt catacctttg accaatgcta
    10741 ttgcagatgc catgattata tcttcattta cagcctctga tttaaagata tttccatttg
    10801 ggtcctggac ttttatggat acatttgcca aagcgtctgt tccgccggtt attgcttcaa
    10861 ggccgtattc cttcaagatt atcgatggat ctatcaaagt ccttattgca tggctaagtg
    10921 catcaactgg accgacacct ttgccttttc cagtcttagt ctgattgtcg atagttactg
    10981 tcacctctgc ttctggctgg agcttgtttc ctgaggtcac tttaacctcc tcgagcttta
    11041 ttatctcttc ttcttttgag aggcctgcgg ctatatctgc tgctatggct gttatgtctt
    11101 ccctcatcac ttccttttgc ttgtcagcaa gctcttttac tttttctatg actttctgaa
    11161 ctttatcttt tgcaaggtca aagcctgcct cttttaaggc ctgctcgata gcatggtggc
    11221 cggaatgctt tccgatgact aggtttgttc caatccatcc aatctgctct ggctgcatta
    11281 tctcataggt acttctactg ttcatgacgc cgtgctgatg gatgcctgct tcgtgggcaa
    11341 atgcgtttgc tccgactatc gctttgtttc tttggacagc aagtcctgta agctgggaga
    11401 ctaactgaga agctttgaag atctgctttg tgttaatagt tgtctctact tcgctgaaaa
    11461 agtcatttct tgttttgaga ttcattacca cttcttctaa agagcaattg cctgcacgtt
    11521 ctcctatgcc gtttatagtg cactctacct gtgttgctcc attctttact gctgcaagtg
    11581 aatttgcgac tgctaaacct aggtcgttat ggcagtggac gctgattatg accttgttct
    11641 tcttgattac gtcacctagc ttgtcgaaga cagtttttat ccgatgacca aactcctctg
    11701 gctctgcata tcctactgta tctggaatat tgatggtggt cgctcctgct tctatcgtag
    11761 ctttgactat atcgcacata tagttgatgt cagtccttgc tgagtcttca gggctgaact
    11821 cgatatcatc gcataaggat ttggcgtgct ttactgcatc cacagccatc tttatcactt
    11881 cttcctttgt ctttcttagc ttctctttta tatggatgtc agaggttgcg ataaaggtgt
    11941 gaatccttgg cttttttgag tatttcactg cttcccaaga cctgtctatg tcttccttct
    12001 tggttctagc caaaccacag atagtagggc cttttacctc ctgtgcaata agctttacag
    12061 cctcaaaatc gccatcagag cttattggaa agcctgcctc tatcacatca actcctaact
    12121 ttgccaactg ttttgctatt atcaatttct caggggtcgt caagctagct cctgggcttt
    12181 gctcgccatc ccttaatgtt gtgtcaaaaa tgattatttt tttcataagt ttcacctttt
    12241 aactgttgat tataaatttt ttgccgctgg cccttaatcc tttcgaacta gggcaatcac
    12301 agaacgagaa gcagagctac taaaaagtca ctggaatttt ttctatatgc tcttgccata
    12361 attgcctgaa atgcttaggt aatatataaa tgttgatggt cactggtatg gttctcgttt
    12421 cttaatatct ctatatggga atcaaagaca ccactccctg attatctcca gggagacaaa
    12481 tgcgctttta ataataagac ggtgatttat ggcatactta tatcattatc attatttctg
    12541 ctcatcgttc ttttggttaa acagagacta atcttatatt tttatgcaat ttgttgtttt
    12601 tgcgatagtc ctattttttt cggatatgat ttttaggtat ttatttataa tctcaaattc
    12661 gataatcatt ttctcaatca cttcatttac ggtttttatc ttgctgaacg agcttgagaa
    12721 aatgtttccg gcttaatagt aaaatcttaa actgaagcat taggtctttt aggcagcttt
    12781 ttcctcactg ttacaaaaat ataaatatca gtatcaatca atttatccta tgctgataac
    12841 aatcaccagg ttattgataa ccttcctgct ttcttttgcc tttggctatg aaaggcagcg
    12901 atcgcataaa acggttggca tagggacttt tatatttgta gcacttggcg catgcatagt
    12961 aggtattatc gctgagtcta tatgggagct aaatcctatc gggcttcttg catctgttgt
    13021 gaccggaata ggttttttag gcgctggcgc acttataaag gggagtgaca aggtttttgg
    13081 ttttacgacc gcaagcgcag tctggctgtt ttcgatattt ggcctcactg taggcatcgg
    13141 caaatatgag atagctcttg cgacctatgt attggtgtgg atagtgattc ttgtcgacaa
    13201 gcattttgag cagcatggta tcggctctta ccagagaagg ttgataatta cctctaataa
    13261 gatgatagag gaaaaagaga taaacaggca tctggtgatg gggacaaaga aatacaagct
    13321 gcttggcgtg gaactaagaa agcaggtaaa tgaatttcag atgacctatc ttgtagaagg
    13381 gacccaggaa agcttgaata agcttgtgaa aagtttgtat aaggaagagt ggctgaaaga
    13441 ggcgaaggtt gaataactaa tgctcaagca tcttttcaaa ccttaatgcc ctttcccttg
    13501 agcattctct ttttggttct gctgtgaaag acttataaac ctctcctttg aatttgcatt
    13561 cgaggttgtt gttgcaaaga ctgattattc cagagattcc ggtcatatat tggcactgct
    13621 catctttaaa caaagaagca atatatttgt cgaagtcttc ctggtagtgc ttcagcttta
    13681 aatgccttaa ttctaactgc tttgctttaa gcctgtcaag actagcttgc gatagcatct
    13741 ttgaccccct tcaacagaaa tccataggat ttctgagcat gcgaatgttt tacaaccatt
    13801 cgcatatatt tacaatatag aaggaattgg tatttaaaat gtttggtttc tgtgtttttc
    13861 ccattcgctc tttatgttct ataacatctc tgaaatgcga cagctaaact atacattttt
    13921 ctcaaacctg tcccaataat ctgtgattgt gccataggtc actccttctt tgcgaatctc
    13981 aagccccttg agggtctgcc acaactcaac
//
LOCUS       DASRUK010000185        18529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2337822, whole genome shotgun sequence.
ACCESSION   DASRUK010000185 DASRUK010000000
VERSION     DASRUK010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 18529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18529
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2337822"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..536)
                     /locus_tag="VFF28_07985"
     CDS             complement(126..536)
                     /locus_tag="VFF28_07985"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45594.1"
                     /translation="MTGLRIGPSHIVGHSLGGAVAIASGQDNEDISDVVSMNPLFPIN
                     YNLVGFGLRALYKGTREIWGISGERSSRGFGLIISFPFLVNAARDPISTYKTIRSICR
                     YQYKDVKIRNPVLILYGTSDEYFSLDQRLIRPNP"
     gene            complement(548..775)
                     /locus_tag="VFF28_07990"
     CDS             complement(548..775)
                     /locus_tag="VFF28_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45595.1"
                     /translation="MFQCRPGYAGQMQVEYLYKKGSGETLLFLHGFGVHPAFYERLLD
                     KLAGRYEIIAPIAYGMNCLGHQPTNIGSMQN"
     gene            872..1276
                     /locus_tag="VFF28_07995"
     CDS             872..1276
                     /locus_tag="VFF28_07995"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZX45596.1"
                     /translation="MAKIHWSVYLILGGVVLFAANYLSNIADPKKYTIFVWFGYFFLA
                     FGTAKFLIWFITRKKEPKSDWQYQQQLGYPRHAQNSNQTNQRRQHTAQDYDHQSNTNQ
                     FVQQHHPSFCTRCGQHLKGVENFCSRCGTRLR"
     gene            1331..1741
                     /locus_tag="VFF28_08000"
     CDS             1331..1741
                     /locus_tag="VFF28_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45597.1"
                     /translation="MADSLQQKHSMFKSQGPEQPAPISSELEGMISRLRVLEERYTNI
                     QTELRVTEENMISRNKKLAAEIKALTSENTELKKEINEIKEKVLLVIKELQGFAKKED
                     LRVLQRYIEMWEPLNFVTHKEVADVIEEKLAEKR"
     gene            1790..2305
                     /locus_tag="VFF28_08005"
     CDS             1790..2305
                     /locus_tag="VFF28_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45598.1"
                     /translation="MALFSKKKNDLPPDMGAQDQGFYGQNYPAYGEQPQYPQEQPQYP
                     QEQPQYPQQQYPQQQEQPQSREMIQEIAEAIVEEKWKEKEKEQNRITEWRDDVTAKVA
                     QLQQQIDDLKSSFESLHRGLLGKISEYDENLTNVGTEIKAMEQVFSKILPSLTESVNK
                     LQRFSEPKKKV"
     gene            complement(2329..2607)
                     /locus_tag="VFF28_08010"
     CDS             complement(2329..2607)
                     /locus_tag="VFF28_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45599.1"
                     /translation="MKAKSLGACILMWIIIATSGCAKISEKEQNCIESGGKVATMTCY
                     CQGVEDFPDVCGSGICTCNPKATKYSKDLKICDCGDGCWDGDKCTTLP"
     gene            complement(2722..3228)
                     /locus_tag="VFF28_08015"
     CDS             complement(2722..3228)
                     /locus_tag="VFF28_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45600.1"
                     /translation="MATFLDISGLSNFSSFFAFAFVWLATYAILKYSRVLGSNEGINI
                     VIGLILGLFVLFSPIATGLIEFISPWFAVVLIFIVFATVALKMFGGGFESLNSLRVIA
                     FVVILLVMVIGSLSYLRQQVIVPGENGTTTDYSKTSTVLFHPKILGVIFIMIIAIFTV
                     VLMAGKQF"
     gene            complement(3229..3828)
                     /locus_tag="VFF28_08020"
     CDS             complement(3229..3828)
                     /locus_tag="VFF28_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45601.1"
                     /translation="MAFRGYNLQDFVMILESWGLTDVMLPFLLIFVIIFAVLQKARIL
                     GEDKKKFNVIIALVMGLLVVIPHVLNYYPPNGDPVDIMNQALPQVSLIAVAVIMLLIL
                     IGLFGGESKWMGSSLSGWIAIAAFIVVLIIFGGAAGWWDSWDWFYGTFGTETVAVVVM
                     VLVFAIIIWWITRGEGKEKEANTLANLGKWVGEAFNPKK"
     gene            complement(3842..4396)
                     /locus_tag="VFF28_08025"
     CDS             complement(3842..4396)
                     /locus_tag="VFF28_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45602.1"
                     /translation="MVSAFRSVIEFFEEIGLYDVVLPFLLVFTIVFAILEKTRVFGSE
                     HIDGKDYPKKNLDAVTAFVIAFFVVASSKLVQIITDVSSYTVILLLMSVLFLMLVGSF
                     MKEGEGGFLKGNWNYAFMIIMFIGIAMIFAYALGWWDAMWAFFRYRTGGEMAGSIILL
                     LIIVVFIWYIVKDEPHKSGAKESK"
     gene            4507..5079
                     /locus_tag="VFF28_08030"
     CDS             4507..5079
                     /locus_tag="VFF28_08030"
                     /inference="COORDINATES: protein motif:HMM:NF014099.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45603.1"
                     /translation="MIDKEDFEKLRKNLKSYDEQRESLIKKSRDVLKLSKQLIYSIHR
                     NNSKEAAALHAQVKKEKQALDKIAAGEKKLIYEGSYSDACQEYAEALTYYGFIAHGKV
                     APISEVDVGEEDYLMGISDLTGELARKAVVVANKEPEEVRKIKDLVEEIFGEFLKFDL
                     RNSELRKKSDAIKWNLKKLEEIIYDIEKKR"
     gene            5076..6275
                     /gene="tgt"
                     /locus_tag="VFF28_08035"
     CDS             5076..6275
                     /gene="tgt"
                     /locus_tag="VFF28_08035"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HZX45604.1"
                     /translation="MNFQITAEANNSKARLGIVKTKHGSFKTPIFIPVATAATLRAVD
                     NKDIEDLGAEAILANTYHLHLRPGDELIKRLGGLHKFMNIDKPIVTDSGGFQAFSLGF
                     AVEHNVGKIADNYLDMKNRRAVKSEPEQKFATVDEEGVCFKDPINGNKIKLTPKKSME
                     IQSNLGSDIIFAFDECTSPLSNKTYTAEALKRTHRWAKECLEYYNKNQALFGIVQGSS
                     YKYLRLKSAKYMASLPFDGFGIGGSLGKSKDDMHNILDWTIPLLPKEKPRHLLGIGAV
                     EDLFNSVERGIDMFDCVAPTRWARRGHMYISPEEGGTKENKFRLNIENKCFEYDAKPV
                     DKTCGCFACKNHTRAYLRHLFKSSELTYFRLSSLHNLNFLLRLMDKIRISIKENSFGR
                     LKKEWLG"
     gene            6330..6485
                     /locus_tag="VFF28_08040"
     CDS             6330..6485
                     /locus_tag="VFF28_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45605.1"
                     /translation="MSWIGELFLELLGAIAEVTAEKNISRKQAYIILAVFMLCLAAII
                     LYFILNS"
     gene            6535..8298
                     /gene="aspS"
                     /locus_tag="VFF28_08045"
     CDS             6535..8298
                     /gene="aspS"
                     /locus_tag="VFF28_08045"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HZX45606.1"
                     /translation="MKRTNTCGELTKKDDAKYVTLCGWVNSRRDHGGVIFIDLRDRYG
                     LTQIVFSTAHGTKAHEEAQHLRREDVLQAKGKVRPRGEGLVNPKLKTGEIEVAIDDLT
                     ILNKADTPPIEVDDRVEINEDMRLKYRYVDLRKPRMQANIALRHRTVKAVRDFYDKHG
                     FLEIETPMLGKSTPEGARDYLVPSRVNPGKFFALPQSPQLFKQLLMVSGFDRYMQVVK
                     CFRDEDLRADRQPEFTQIDVEMSFIDEEDIYSLHEELMKHIWKETLNQDIKIPFQRMT
                     YQEAMGKYGSDKPDIRFNLELTDVTDIMKGSDFKVFTDNIQSGGIVKAINVKNSPMSR
                     KDIDELIEFVKIYDAKGLAWIKVTDKGLESSIVKFLSEKIQKELVEKLQAKQGDLLLF
                     VSDHKHFIVNTSLGFLRLHLAKKLGLIPKDTFKFLWITDFPLFEYDEDEQRHVAVHHP
                     FTSPKDEDIQYLESHPEKVRAKAYDLTLNGTEIGGGSIRIHKRELQSKIFKLLGIREE
                     EAQNKFGFLLDAFKYGAPPHGGIAFGVDRLSALLTGNESIREVIAFPKTKAAESLMEG
                     SPSEVDEKQLKELHVKIDLAL"
     gene            complement(8306..9100)
                     /locus_tag="VFF28_08050"
     CDS             complement(8306..9100)
                     /locus_tag="VFF28_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45607.1"
                     /translation="MVLTNTCEPIKSDYEQAKAEGGLISLIKNSTLPPLVRDSSVILF
                     RQLDRALYTEQNIAFPTVGSHTISAPVPRLQALRDNPYYIHYILSAICGVDDVKGKPN
                     SLIPPVEKKYSKVRGGINRLYGKFEHELRRSKEFPDINNPEEDEHIKWFLQEFYDWNV
                     RPALTAARVARDNKLITPKNSEAFDDLMGVYRTEMRKIHGELVGFNGLDGIARPAAEI
                     LKKAGTIDNMFTSQESWERKIAVSLYSSAFFLNYVALDRERVKNAA"
     gene            9233..9781
                     /locus_tag="VFF28_08055"
     CDS             9233..9781
                     /locus_tag="VFF28_08055"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HZX45608.1"
                     /translation="MKLYFVTSNLGKVKEFRQILEPKVEVEHIEMQYPELRSDDPEEI
                     AQMSAKQLADKFKRPIVVEDSGLFIKALNGFPGTCSSYIHKRIGLKGILKLMKGEKGR
                     EAMYMSAVAYCEPGKAPVSFLGTEKGTIAAKIRGKNGFGHDPIFIPEGKEKTYGELDN
                     YAELKQFRRRAVKKLLEYLENK"
     gene            9854..10726
                     /locus_tag="VFF28_08060"
     CDS             9854..10726
                     /locus_tag="VFF28_08060"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HZX45609.1"
                     /translation="MAFVRRKSVNGKLYYYLVKSVRNGKNIERYIGLNQPGKKDIQLF
                     EEEHSILASFLEKSEPFLSEVKKAYRKKLARGSKDEITQFEEMIVTQLTYNTNRIEGS
                     RLTYKDTNLLLNHGIVPGNKTIRDVKETENLKKVYLFSKGYKKDIDESLILSLHRILK
                     EGISEDAGAYRTAGVMVGDMVGLNCKLISLEIKNLLAWYNKNKNKLHPLELTAQFHCT
                     FERIHPFFDGNGRVGRLLLNHTLSRKGYPPLIIQDKNKKRYYNALIKGQKGNYYLMIK
                     YMASELENMLPILR"
     gene            10780..11274
                     /locus_tag="VFF28_08065"
     CDS             10780..11274
                     /locus_tag="VFF28_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HZX45610.1"
                     /translation="MKITIMQGDITGLCVDGIVNPANSYGYMGGGVAAALKEAGGEEI
                     ELEAISHAPIPIGHAILTTAGTLKASYIIHSPTMAQPAEKIDASNVKEATWAALEAAD
                     ERHLKKIAIPGMGTGEGEVPKRKAAEAMIETIVNFNEQYLVEAILIDKDQEMVDEWKR
                     LITP"
     gene            11306..11821
                     /gene="pgsA"
                     /locus_tag="VFF28_08070"
     CDS             11306..11821
                     /gene="pgsA"
                     /locus_tag="VFF28_08070"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HZX45611.1"
                     /translation="MNLPNRITLMRMFLIPVLIILLYFRSKYLDYAAAAIFILLAATD
                     ALDGYIARKTKQVTRLGKLMDPLADKLLISAALIFLIKRGIPEWMAFVIIAREFAVTG
                     LRLVAQSKGTTMEATGWGKVKTFSQIVAIVAVMINFPFFYYFILTATIITVISGIVYF
                     YNARSMLKENE"
     gene            11814..12338
                     /locus_tag="VFF28_08075"
     CDS             11814..12338
                     /locus_tag="VFF28_08075"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZX45612.1"
                     /translation="MNNEEIITIPNFITLFRIVCIPFFILSLVYNKDAWAVLIFAAMS
                     LSDALDGISARLTNQKTKIGALLDTTADNLVVFSTLLTFTIIRGMIPFYITIALFVAF
                     ILCALAKIIYVKKGNSTNPTIMGKLAIAFAYLTGIAYLIDFQYKLVFSLILVFFAYAT
                     VATYLAKDIRLLKK"
     gene            12340..13800
                     /locus_tag="VFF28_08080"
     CDS             12340..13800
                     /locus_tag="VFF28_08080"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZX45613.1"
                     /translation="MDTILDCYTDEPAGLGVPPYLGTYPRYLAGYLDDPVYLTIDDLR
                     YLKYYNLDEKKKEISHKSNIKIYNLTSNINRIKDILKNTTRLFIILGVHTPGKYLSAI
                     PGTLDEVEALISDLDCEKILTGPAIFGTQLEGGRFFEKEKKGVFQKNELLNFSYEEIA
                     DYGVKGANIISQIPSLRIIEIETGHGCSRKKACSFCTEPIKHKLEFRQNKDILKEIKT
                     FYDLGIRHFRLGKQSDFYMLPDAAALLKDIRSSCPEIDVLHIDNVNPQSVNSEKGIEL
                     TKAIVKYCTEGNIAAFGVESFDREVCKENCLNSTPEESYEAVKTLNKYGSKRGPNGMP
                     IFLPGINILFGLKGETKNTHKENMAWLKRILDENLLLRRINIRQVTIFENTPLYKEAG
                     NKFLRKNRRFYWKWRNDIRQNIDLPMLNKIVPIGSQLSQVRTEIYDGNTTFARQVGSY
                     PLIIGIKQRLPLNQYCTVKVTGHMLRSITAEVDTEP"
     gene            13841..14983
                     /locus_tag="VFF28_08085"
     CDS             13841..14983
                     /locus_tag="VFF28_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45614.1"
                     /translation="MRKAQLAHPLLYAFIAIVSITLLSYGAVQIIKLASTEDKLQLLN
                     FQKTLANSIKKQAAYNYGTVDEKSFALSPSIRSVCFIDKTKAISPLVKENLNPQINKY
                     EDKTIFFEPFEKFSPIEIEGFELEENPLCLEPVNSVLRLSLTSNGNITRLGTFRPSDK
                     NIDCVSVLYNSNNSINIVFLAYGYNKFEDFRNDVATYIDLFQSTEPFNTSNINFYRID
                     KLDQLNCEISQYIRCSEYEVKKLASSCPNDYIIILADRSKVLDILSQVRSSAVSNMAK
                     INTADPHKLVVLHEFGHIFGNLADEYVDEKYYKSIKFDPSDYPNCDYPPECGKWSTVE
                     GTGCFKGCSLDKFSRSTEDSLMRKLDVKNFGPLNEKILLERLSHYD"
     gene            14976..15491
                     /locus_tag="VFF28_08090"
     CDS             14976..15491
                     /locus_tag="VFF28_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45615.1"
                     /translation="MIKSLIILLLLAPLALAEVQESRVIIANFNYDDGLVTYKEKTIK
                     FGYAPDYKLEPEDGYKAELLSFDDKELASFRFDIPLKLYFDMSDPLLKTLSGGMVILN
                     ETDFALVFPYDERAEKIVISNPRNYTILEVPLVEEKLSISRASYGFYILPFAVLFAIF
                     AFMAYIKFRKK"
     gene            15971..16744
                     /locus_tag="VFF28_08095"
     CDS             15971..16744
                     /locus_tag="VFF28_08095"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HZX45616.1"
                     /translation="MKQKAIIYHFSGTGNSLSIAKKIAEKLDAKLLSVAAQKDKPLEQ
                     AEITGIVFPVYCWGLPSIVANFSKQLKSDSYYFGVATFGGMVGNSIIQLKDILKSNKQ
                     VLSAGFGIRMPGNYFPLYGAKPLEKQKKMFDKAEEAVLDIVKIVQQKKISKLSKSNFL
                     VNSLLGKFLYKKMMKNMKAADKNFWVDDNCNGCGTCAELCPVNNLTLKDKKPVWGHGC
                     EQCMACLQWCPKQAIQFKQIKERKRYHNPDIALKEMLLR"
     gene            complement(16746..17369)
                     /locus_tag="VFF28_08100"
     CDS             complement(16746..17369)
                     /locus_tag="VFF28_08100"
                     /inference="COORDINATES: protein motif:HMM:NF015827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HZX45617.1"
                     /translation="MKKIAIVLLMASALGLITACQPKACPEDAKVCPDGTTVGREGPN
                     CEFPECPGGDAQLPNPASVFCEEQGGKVEIVNEAGGQRGICVLADGTRCDEWAYFRGE
                     CPECGECPQLSPAPPHCPDGVVVPGGKDKCGCTLPAQCVEKNVCTAESREGEFCTKEY
                     RPVCGWFNESIKCIAYPCAITASNPCEACHNPQVEYWTEGECPEVSK"
     gene            complement(17562..18302)
                     /locus_tag="VFF28_08105"
     CDS             complement(17562..18302)
                     /locus_tag="VFF28_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45618.1"
                     /translation="MNKRWYALIITAFCLAVLGLYINYKISLSRPQGSITLSLPFSPE
                     DEPTGMMPAGEQVMHNFYLGHPGVDFNWRHEAFIIASANATVRKIESRNNQWDIETVS
                     GDYAIRYTELGDYNRDLREGTQIKKGDLIGTTNCGKVDEGLNCMVHFEFASRLSIRYP
                     WLERLCPYTYFDSISKERIDQIWNESDWVEMKTMYPEICSGGYKGRDSIQDALRIDAE
                     KKFKFNKSVIDEYNRLREADYQRRLQLK"
     gene            complement(18346..18457)
                     /locus_tag="VFF28_08110"
     tRNA            complement(join(18346..18378,18397..18402,18419..18457))
                     /locus_tag="VFF28_08110"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18420..18422),aa:Ile,seq:gat)
BASE COUNT         5962 a         3872 c         3785 g         4910 t
ORIGIN      
        1 ccttattgtg tgaatttcta tgtgtgccat aaaattatca cctttgaaat aattaacggc
       61 tacctatatt taaacttatc ggtgatagaa tctatatgta gcaggcaata tattaagaag
      121 tattattaag ggtttggtct aattaacctt tgatccaggg aaaaatattc atcacttgtt
      181 ccatacaaga tgaggactgg attgcgtatc ttaacatcct tatattgata ccgacaaatg
      241 cttcttatgg tcttgtatgt gcttattggg tctctggctg cattgacaag gaatgggaac
      301 gaaataatca acccaaatcc cctcgaactt ctttcaccac ttatacccca tatttctctg
      361 gttcctttat atagcgcacg aaggccaaat ccaacaagat tataatttat agggaacaag
      421 ggattcatag atacaacatc gcttatatcc tcattatctt ggcctgaggc aatagcaaca
      481 gcgcccccaa gtgagtggcc gacaatatgg gatggcccta tacgaaggcc agtcataaaa
      541 tccgaggtta attctgcata cttcctatgt tggtaggttg atgcccaagg cagttcattc
      601 cataagcgat tggagcaata atctcatacc taccagctag cttatcaagc agtctttcat
      661 aaaaggctgg atgaacaccg aagccatgca aaaacaacaa tgtttcgccg gatccttttt
      721 tataaaggta ttcgacctgc atttgtcctg catatccggg tctacattga aacatacgat
      781 gagaatattc aattactata taattgctac tctaaaatag ttccggtata ttccgcaact
      841 ttaatatatg actcagggat aggtcatgca tatggcaaag atccattgga gtgtatatct
      901 aattttagga ggagtcgttc tttttgcagc gaattaccta tcaaatattg ctgacccaaa
      961 gaaatatact atctttgtct ggtttggcta tttcttcctt gcttttggaa cagcaaagtt
     1021 tctcatctgg tttatcacaa gaaagaaaga gccaaaatca gattggcagt atcagcagca
     1081 gttgggctat cccaggcatg ctcagaatag caatcaaacc aaccaaaggc gccaacatac
     1141 tgctcaagac tatgatcatc aatccaatac caatcaattt gttcaacagc atcatccatc
     1201 cttctgcacc cgctgtggcc agcacctaaa aggtgttgaa aacttctgct caagatgcgg
     1261 cacaaggctt aggtaaacca ctatcagata ctaacataag tttataactt atctgctttc
     1321 taatcagttt atggcagata gccttcaaca aaagcattct atgttcaagt ctcaaggtcc
     1381 agagcagcca gctccgatat catcagagct ggaaggtatg ataagcaggc ttagggtcct
     1441 tgaggaaaga tataccaata tccagacaga gcttagagtg acagaagaga atatgatctc
     1501 aagaaataag aagctggcag cagagataaa agcccttaca tctgaaaata ctgagttaaa
     1561 aaaagagata aacgagataa aggaaaaagt cctgttggtg ataaaagagc ttcagggctt
     1621 tgccaaaaaa gaggacttga gggttcttca aagatatatc gagatgtggg agcctcttaa
     1681 cttcgtcacc cataaggaag ttgctgacgt gatagaagaa aagctggcag aaaagagata
     1741 gatttatata ttttaaacag gcatatttca tgaaaaagag gtaaatgtaa tggcgttgtt
     1801 ttcaaaaaag aaaaatgacc tgccgcctga tatgggggct caggatcaag gcttctacgg
     1861 tcaaaattat cctgcctatg gcgagcagcc gcaataccct caggaacagc ctcagtaccc
     1921 tcaggagcag ccgcaatacc ctcagcagca ataccctcag cagcaagagc agccccagtc
     1981 aagggagatg atccaggaga ttgctgaagc aattgttgaa gaaaaatgga aagagaagga
     2041 aaaagagcaa aacaggataa cagaatggag agacgatgtt actgcaaaag ttgcccagct
     2101 tcagcagcag attgatgatc taaagtcctc atttgaatcc ttgcataggg gattactagg
     2161 aaagatttct gaatatgatg agaacctgac aaacgtaggc acagagataa aagcaatgga
     2221 gcaggtcttc tcaaagattc tgccttcatt gacagagagc gtgaataagc tccaaagatt
     2281 ttctgagcca aagaagaaag tgtaggatct aactcattat cctatatttt aaggcaaagt
     2341 agtacactta tcaccgtccc aacaaccatc tccgcagtca caaattttta aatccttaga
     2401 atattttgtt gctttagggt tgcatgtaca tatacccgac ccgcaaacgt ctggaaaatc
     2461 ctcaactcct tggcaatagc atgtcattgt agcaaccttg cctccggact ctatgcaatt
     2521 ttgttctttt tctgagattt tggcacatcc agaagttgcg ataattatcc acattaagat
     2581 gcatgccccg aggcttttag ctttcatatt attccttcgc ttgattgctc aaactgatac
     2641 ctaccctgca gcttggctag cggtttggga ttttttatta cttaagcctt cttaagaaag
     2701 gctcttgaga atacccctaa tttaaaactg ctttccagcc atgagcacaa cagtaaatat
     2761 cgctattatc attataaata tcacgcccag gatctttgga tgaaacagca ctgtcgatgt
     2821 cttgctgtaa tctgtggttg tgccgttttc tccaggaaca atgacttgtt gcctgagata
     2881 gcttagtgaa ccaattacca ttaccagtag aatcaccaca aatgctatta ccctaagcga
     2941 gtttaagctt tcaaatccac cgccaaacat ctttaatgca acggtcgcaa agactatgaa
     3001 tatcaggaca acagcaaacc atggggagat aaactctatc agccctgtgg cgattggcga
     3061 aaacaaaacg aaaagcccaa ggattagccc tatcactata tttattcctt cattagaccc
     3121 aagaacccgc gaatatttca gtatagcata agttgcaagc caaacaaaag caaatgcaaa
     3181 aaaagaagaa aaattcgaaa gtccgcttat atcaaggaag gtagccatct acttctttgg
     3241 attgaatgct tctccaaccc atttcccgag gtttgcaaga gtatttgcct ctttttcctt
     3301 tccttctcct cttgttatcc accagattat gattgcaaat acaaggacca tcaccactac
     3361 agccacggtc tctgtcccaa atgtgccata gaaccaatcc cagctatccc accagccggc
     3421 agctcctcca aagataataa gcacaacgat aaatgctgcg atggcaatcc acccggataa
     3481 tgaagagccc atccattttg actctcctcc aaaaaggcct atcaatatta gcagcattat
     3541 tactgcgaca gcgatcagcg aaacctgagg cagagcctgg ttcataatgt cgaccggatc
     3601 tccattagga ggataataat tcaaaacatg aggtattaca acaagaaggc ccatgacaag
     3661 tgcaatgatt acattgaact tcttcttatc ctcgccaagt atccttgctt tttgaagcac
     3721 agcaaagatg atgacaaata tcaaaaggaa tggcagcatc acgtcagtga ggccccacga
     3781 ttccaatatc atcacaaaat cttgaagatt gtatccacga aatgccattt tttacacctc
     3841 tttacttcga ctcctttgcc cctgatttgt ggggttcatc ttttactata taccaaatga
     3901 agacaacgat tatcaacagc aggattatcg agccagccat ctctccgcct gtcctgtatc
     3961 tgaagaatgc ccacattgca tcccaccagc caagagcgta tgcaaagatc atcgcaatac
     4021 ctataaacat tattatcatg aaggcatagt tccagttgcc cttaagaaag ccgccttctc
     4081 cttccttcat aaaagagccc acaagcataa ggaacaaaac agacataagc agtagtatca
     4141 cagtatacga agacacatca gtgattatct gcacaagctt tgacgaggct accacaaaga
     4201 atgcgatgac aaaagcagtg accgcatcaa ggttcttttt tggatagtcc ttgccgtcga
     4261 tatgctcaga gccaaagacc cttgtcttct caagtatcgc aaacacgatg gtaaatacga
     4321 gcagaaaggg cagtacgacg tcatataatc ctatctcttc aaaaaattct atgacgctcc
     4381 taaatgcact aaccatacgc taattcctgg gacgtttgta tataaacttt gcgtttttcc
     4441 tattctatag cccttggcga aaaattttac aaatatttat ataccccaaa gcagccacat
     4501 actgttatga ttgacaaaga agattttgaa aagctcagga aaaacctaaa aagctatgat
     4561 gagcaaaggg aatcacttat caaaaaatca agggatgtct taaagctttc aaagcagttg
     4621 atatattcca tccataggaa taattctaaa gaagcagcag cgctccacgc tcaggtaaaa
     4681 aaggaaaagc aggcgctgga caagattgca gcaggagaaa aaaagcttat ctatgaaggc
     4741 tcatactcag acgcctgtca ggaatatgca gaagccctga cttattatgg ttttatcgct
     4801 cacggaaaag tagcgccgat ctcagaggtt gatgtaggtg aagaagacta tctgatgggt
     4861 atctccgacc ttacaggcga gcttgcaaga aaagcagttg ttgtcgcaaa taaagagcct
     4921 gaagaagtaa gaaagataaa agatctggtt gaagagatat ttggagaatt ccttaagttt
     4981 gacttgagaa actcagagtt gaggaagaaa tcagatgcta ttaagtggaa tctaaaaaag
     5041 cttgaagaga taatatacga cattgaaaag aagcgatgaa tttccaaata acagctgaag
     5101 caaataattc taaggcaagg cttggcatag taaagacaaa gcatggcagc tttaagactc
     5161 ctatcttcat cccggttgcg acagcagcaa cacttagggc agtagataac aaggacatag
     5221 aagatcttgg cgcagaagcg attcttgcaa acacttatca cctccatcta aggccaggcg
     5281 acgagttgat aaagcgtctg ggaggcctgc acaaattcat gaacatagac aagccgattg
     5341 ttacagactc gggcggcttt caagctttct cccttggatt tgcagttgag cataatgtcg
     5401 gaaagatcgc agacaactat ctggacatga agaacagaag agccgtaaag tcagagccag
     5461 aacaaaaatt tgctacagtt gacgaagaag gtgtctgttt caaggatccg ataaatggaa
     5521 acaagataaa gctcactcca aaaaagtcca tggagataca gtcaaacctg ggctcagaca
     5581 taatctttgc atttgatgaa tgcacaagcc cgttatccaa caaaacctat acagcagaag
     5641 ctctcaaaag aacccacagg tgggcaaaag agtgcttgga atactataat aaaaaccaag
     5701 ccctttttgg tatcgtccaa ggctcatcat ataaatacct aaggctaaag agcgcaaaat
     5761 atatggcaag tcttcccttt gatggttttg ggataggagg aagccttggc aagtcaaaag
     5821 atgatatgca taatatatta gactggacca ttcccttgct gccaaaagaa aagcccaggc
     5881 atctcttagg gataggtgca gtcgaagact tgttcaactc agtagagcga ggtatagaca
     5941 tgtttgactg tgttgcgcca accagatggg caagaagagg ccacatgtat atatccccag
     6001 aagaaggcgg cacaaaagag aacaagttca ggctgaacat agaaaacaaa tgctttgaat
     6061 acgacgcaaa gccagttgac aagacatgcg gctgctttgc ctgcaaaaac cacacaaggg
     6121 cttatctacg ccaccttttc aagtcaagcg aacttactta ttttaggtta tcatctttgc
     6181 ataatcttaa tttcctattg cgccttatgg acaagatcag gatttccatc aaggagaatt
     6241 cttttggtag gttgaagaag gaatggttag gctagcctgc tatgattttt gacaattttt
     6301 ttatagcgat atatattttt gaggcttaga tgagctggat aggcgaatta tttttagagc
     6361 tattaggtgc aatagcagaa gtaacagcag aaaagaatat aagccgtaag caggcttaca
     6421 ttatcttggc ggtctttatg ttatgtttgg cagcaataat cctctacttt attttaaata
     6481 gttagctatc gtcaacataa acatttataa attgcataat aaatctctaa caccatgaaa
     6541 aggacaaata cctgcggaga gctgaccaag aaagatgacg ctaaatatgt aactctttgc
     6601 ggctgggtaa acagcagaag agaccatggc ggagttatat tcatagacct aagagacagg
     6661 tatggcctca cccagatagt atttagtaca gcccacggca caaaagctca cgaagaagct
     6721 cagcacctga gaagagaaga tgtccttcag gcaaaaggaa aagtaaggcc aagaggagaa
     6781 ggccttgtaa atcccaagct aaagacagga gagattgaag ttgcgattga tgatcttacg
     6841 attctaaaca aagcagatac tcctccgata gaggttgacg acagggtaga gataaacgag
     6901 gacatgaggt taaaatacag gtatgttgat ctaagaaagc caaggatgca ggcaaatatt
     6961 gcgctaaggc acaggacagt aaaagcagta agggatttct atgataagca tggattttta
     7021 gagatagaga caccaatgct tggaaaatca acaccagagg gagcaagaga ctatcttgtc
     7081 ccgtcaagag taaaccctgg aaaattcttt gctcttccgc aatcccctca actattcaag
     7141 caattgctca tggtaagcgg ttttgaccgt tacatgcagg ttgtaaaatg cttcagggat
     7201 gaagacttaa gggcagacag gcagcccgaa ttcacccaga tagatgtcga gatgtcattc
     7261 attgacgaag aggacattta ctccctccat gaagaattga tgaagcatat atggaaagag
     7321 actttaaacc aggacataaa gattccgttc caaaggatga cttatcagga agctatggga
     7381 aagtatggct cggacaagcc tgacataaga ttcaatttag aacttacaga cgtaacagac
     7441 ataatgaaag gctctgactt caaagtattt acagataata tccagtcagg tggtatcgtt
     7501 aaagctatca atgtaaaaaa ttcaccgatg tcaagaaaag atattgatga gctcatagaa
     7561 tttgtaaaga tttacgatgc aaaaggcctt gcatggataa aagtgacaga caaaggacta
     7621 gagtcatcga ttgtaaaatt cttaagtgag aagattcaaa aagaattggt agaaaagctg
     7681 caggcaaaac aaggagacct gttattgttt gtctcagacc acaagcactt tattgtaaac
     7741 acctcacttg gttttctaag gctccatctt gcaaagaagc taggccttat tccaaaagat
     7801 actttcaaat tcctttggat aactgacttt ccgctgtttg aatatgacga agatgagcag
     7861 cgccatgttg ctgtgcatca cccatttaca tcgccaaagg atgaagacat ccagtatctt
     7921 gaatcccatc cggagaaagt cagggcaaaa gcatacgacc ttaccttgaa tggtacagag
     7981 ataggcggag gatctatcag gatccataaa agagagcttc aatcaaagat attcaagctt
     8041 ttaggcatac gtgaagaaga agcccagaat aagttcggat tcctgctgga cgctttcaaa
     8101 tacggcgctc cacctcacgg tggcattgca tttggcgtag acaggctctc agcccttctt
     8161 acaggaaacg aatccataag ggaagtgatt gcattcccaa agacaaaggc cgcagaaagc
     8221 ttgatggaag gctcaccctc agaagtcgac gaaaagcagc taaaagaatt acatgttaaa
     8281 atagatttag ctttgtaagt gattttcaag cagcattctt cactctttcc ctatcaagtg
     8341 cgacgtaatt taagaagaat gcggatgaat ataggcttac agcaattttt ctctcccatg
     8401 attcttggct cgtaaacata ttatcgatag ttcctgcttt cttgagtatc tccgcagcag
     8461 gacgagcgat gccatcaagc ccgttaaatc ctaccagctc accgtggatt tttctcatct
     8521 ctgttctgta tacacccatc agatcatcaa atgcttcact atttttagga gtgatcaact
     8581 tattgtcacg ggcaactctt gcagctgtca gcgcaggtct gacattccaa tcataaaact
     8641 cctgcaaaaa ccacttgata tgctcatctt cttcaggatt attgatatct ggaaactcct
     8701 ttgatcttct gagttcatgc tcaaattttc catacagtct attaatccct cccctaacct
     8761 tagaatattt tttttctact ggaggtatta gagaattggg cttgcccttc acatcatcta
     8821 caccacagat agcactcagg atatagtgga tataataagg attatctctg agcgcttgca
     8881 gcctaggcac aggagcgctt atggtatgac tcccgaccgt aggaaatgca atgttttgct
     8941 ctgtatacaa agccctatcc agttgtctaa acagaattac tgaggaatct ctcactaatg
     9001 ggggcagggt agaatttttt attaggctga tcagccctcc ctctgctttg gcctgttcat
     9061 aatctgattt gataggttcg catgtattgg ttaataccat tttttgttac cacttaggta
     9121 gagattagtc acattttact tttaaatctt tctataaatt atatatttta tataaaagta
     9181 gcagtaagaa gggataataa gatatttaaa tagatttcct ttaacttaat agatgaaact
     9241 ctacttcgtc acctctaacc tgggcaaggt caaggaattc aggcagattc tagagccaaa
     9301 ggtggaagtt gagcacatag agatgcaata ccctgagcta aggtcagacg acccagagga
     9361 gatagctcag atgtcggcaa agcagctcgc agataaattt aaaaggccca tagttgtcga
     9421 agactcagga cttttcataa aagcactgaa cggctttcca ggaacatgct cttcatacat
     9481 tcacaaaagg atagggctaa aaggcattct caagcttatg aagggagaaa aaggcaggga
     9541 agcgatgtac atgtcagcag tcgcatactg cgagccagga aaagcacccg tctccttcct
     9601 tggcacagaa aaaggcacga ttgcagcaaa gatcagggga aaaaacggct ttggccacga
     9661 ccctattttt atccctgaag gaaaagagaa aacctacggt gaactggata actatgccga
     9721 gttgaaacag ttcagaagaa gagcagtaaa gaaactgctt gaatacctgg aaaataagtg
     9781 accaagaaag gcttatatac ctgtgatgac taattcttaa taattaccaa aaaataatta
     9841 aatgtgatga ctaatggctt ttgtaagaag aaaatcagtt aatggtaagt tatactatta
     9901 tcttgtaaag tctgtgagaa atggaaagaa tattgaaaga tacattggat taaaccagcc
     9961 aggcaaaaaa gacattcaac tttttgagga ggagcattcc atattagcat cgtttcttga
    10021 aaaaagcgag ccgtttttgt cagaagttaa gaaggcatat agaaaaaaac tggccagagg
    10081 ctcaaaagat gagataaccc aatttgaaga gatgattgtc acacagctga cgtacaatac
    10141 aaacaggatt gagggctcaa ggttaactta taaggatact aacctgctgc ttaaccatgg
    10201 aatagttcca ggcaacaaga ctattaggga tgtcaaggag actgaaaacc taaaaaaagt
    10261 ttatcttttt tcaaagggat ataaaaaaga catcgatgaa tctcttatct tgtcactgca
    10321 taggatactt aaagagggaa tttcagaaga tgcaggagct tacaggacag ctggtgttat
    10381 ggttggagat atggttggct tgaactgcaa actgatatct ttagagataa aaaacttgct
    10441 tgcatggtac aataaaaaca aaaacaagct tcatccgctt gagctcacag cgcagtttca
    10501 ttgcacattt gaaaggattc atccattctt cgatggcaat gggagagtag gaaggctttt
    10561 gctcaatcac actttgtcaa ggaaaggata tccgccgctg atcatccagg acaagaataa
    10621 aaaaagatat tacaacgctc tgattaaagg acaaaaaggg aattattacc ttatgataaa
    10681 gtatatggcc tctgagcttg agaatatgtt gcctatttta aggtaaggtt taccttaacc
    10741 cctacctttt taactatcaa cctctttctt ttctccctca tgaaaataac cattatgcaa
    10801 ggagacatca caggattatg tgttgatggc atagtaaacc ctgccaattc ctatggctat
    10861 atgggtggtg gtgtcgcagc tgcgctcaaa gaagcaggcg gagaggagat agaattagag
    10921 gcaataagcc atgctcctat ccccattggc catgccatct tgaccacagc aggaactcta
    10981 aaagcaagct atatcatcca ctcgcccact atggcccagc cggcagaaaa gatagacgca
    11041 agcaatgtca aagaagctac atgggcagct ttagaggcag cagacgagcg ccatctaaaa
    11101 aagatagcta tccctggcat gggcacaggc gaaggcgaag tcccaaaaag aaaagccgca
    11161 gaagcgatga tagaaacaat cgtcaatttc aacgaacaat atttggtaga agccatcctc
    11221 atagataaag accaagagat ggtcgatgag tggaagcgcc tgattacccc ataacattta
    11281 aatatatatt tccgaaagca tatctatgaa ccttccaaac aggattacat tgatgaggat
    11341 gtttctcatc cctgtcctca tcatccttct atattttagg agcaaatatc ttgattatgc
    11401 agctgcggcg attttcatct tgcttgcagc aacagatgca cttgatggct atattgcaag
    11461 aaagacaaaa caggtcacaa ggcttggaaa actgatggat cctcttgcag acaagctcct
    11521 tatctcagca gcccttatct tcctgataaa aagaggtatc cctgaatgga tggcctttgt
    11581 cataatcgca agggaatttg cagtcacagg cttaaggctt gtcgctcaat caaaaggaac
    11641 caccatggaa gcgacaggct ggggaaaagt aaagactttc tcgcagattg tggcgattgt
    11701 ggctgttatg ataaatttcc cgttcttcta ttatttcatc ctgacagcga cgattatcac
    11761 ggtaatctca ggcatcgtct atttttacaa cgcaaggtcg atgttgaagg aaaatgaata
    11821 acgaggaaat aataactata cctaatttta tcacgctctt ccgtatagtt tgcatcccat
    11881 ttttcatcct atcgttggta tataacaagg atgcatgggc tgtcttgatt tttgccgcca
    11941 tgtcgctaag tgatgcattg gacgggattt ccgcaaggct gacaaaccaa aagacaaaga
    12001 ttggcgctct tcttgacacg acagcagaca acctggtcgt tttctctacc cttcttactt
    12061 ttacgataat caggggaatg atcccgttct atattaccat cgccttattc gttgcattca
    12121 tcctttgtgc gcttgcaaag attatttacg tcaaaaaagg aaactcaaca aaccccacta
    12181 taatgggaaa gctggctata gcctttgcat acctaactgg aatcgcctat ttgattgatt
    12241 tccagtataa gcttgttttt agtttgattc ttgtattttt cgcctatgcc acagttgcaa
    12301 cttaccttgc caaggatatc aggcttttga agaagtaaga tggacaccat acttgattgt
    12361 tatacagatg agccggcagg ccttggagtt cccccatacc tcggcactta ccccaggtac
    12421 ttggcaggtt atttagatga ccccgtctac ctgacaatag atgatttgag atatctcaag
    12481 tactacaatt tagacgaaaa aaagaaagag atatctcaca agtcaaacat aaagatatac
    12541 aaccttacca gtaacataaa caggatcaag gacattctaa aaaacactac ccggcttttc
    12601 atcatccttg gcgttcatac ccctggaaaa tacctttcag cgatacctgg cacattggat
    12661 gaagttgaag cattgatctc tgacctggat tgcgaaaaga tacttacagg tccggcaatt
    12721 tttggcaccc agcttgaagg aggaagattc ttcgaaaaag agaaaaaagg agttttccaa
    12781 aaaaatgaac ttctcaattt cagctatgaa gagatagcag attatggggt aaagggagca
    12841 aatattatct cccagattcc tagtttaagg ataattgaga tagagacagg ccatggctgt
    12901 tcaagaaaaa aagcttgcag cttctgcaca gagccgataa agcacaagct tgagttcagg
    12961 cagaacaaag atatcctaaa agagataaag acattctacg atttgggcat aaggcatttt
    13021 aggctcggca agcaatctga cttctacatg ctgccagatg cagccgccct gctaaaagac
    13081 atacgctcgt catgccctga aatagatgtc cttcacatag acaacgtcaa ccctcagagc
    13141 gtcaattcag aaaagggtat cgaactgaca aaagcgattg tcaaatactg cacagaagga
    13201 aacatcgcag cattcggtgt cgaaagcttt gacagggaag tctgcaagga aaactgcctc
    13261 aactcaacac ctgaagagtc atacgaggca gtcaaaaccc ttaacaaata tggcagcaaa
    13321 agaggtccaa acggtatgcc tatattcctt ccagggataa acatcctttt tggcctgaaa
    13381 ggcgagacca aaaacaccca caaggaaaac atggcctggc taaaaaggat ccttgatgaa
    13441 aacctcttgc ttagaaggat aaacataagg caggtgacta tatttgaaaa caccccctta
    13501 tacaaagaag caggcaacaa gttcctaaga aaaaaccgca ggttctattg gaaatggcga
    13561 aacgatataa ggcagaatat cgacctgcct atgctaaaca agatagttcc gataggctcg
    13621 cagctctctc aggtaaggac agagatatat gatggcaaca ccacatttgc aaggcaggtc
    13681 ggctcttatc cgctgataat cgggataaaa cagcgcctgc cattaaacca atactgcaca
    13741 gtcaaggtca caggccatat gctaagaagc ataaccgcag aagtggatac agaaccataa
    13801 acagctaaat ttaaatatct ttgagcaaga aaagtaaata atgagaaaag ctcagctcgc
    13861 tcatccttta ttgtatgcct ttatcgccat tgtatctata accctgctat cctacggcgc
    13921 tgtccagata ataaaattgg caagcacaga agataagctg cagctcctaa acttccaaaa
    13981 gacccttgct aattccataa aaaagcaggc tgcctataat tatggcacag ttgacgaaaa
    14041 aagttttgcc ctaagcccca gtatacgatc ggtttgcttc atagacaaaa caaaagcgat
    14101 atctccgtta gtaaaggaaa atcttaatcc acagataaat aaatatgaag acaagactat
    14161 tttctttgag ccctttgaaa aattctcacc tatcgagata gaaggctttg agctggagga
    14221 aaaccctctt tgccttgagc ctgtaaacag cgtgttaagg ctgagcctca caagtaatgg
    14281 taatatcaca cggcttggta cattcaggcc ctcagacaaa aacatcgact gtgtatctgt
    14341 attatacaac agcaataact caataaacat cgtcttcctt gcttatggct ataacaaatt
    14401 tgaagacttt aggaatgacg tagcaacata tattgatctc ttccaatcga cagaaccctt
    14461 caacacaagc aatataaact tttaccggat tgataagctt gatcagttaa attgtgagat
    14521 aagccaatac atcaggtgca gcgagtatga ggtaaaaaaa ctggcttctt cctgcccaaa
    14581 tgattatatc atcattttag cagataggag caaggtgtta gacatattaa gccaagtaag
    14641 gtcaagtgca gtctcaaaca tggcaaagat aaacaccgca gatccccaca agctagtcgt
    14701 tctccatgaa tttggccata tctttggaaa ccttgccgat gagtatgttg atgaaaaata
    14761 ttacaaaagc ataaagtttg atccaagcga ttatccaaac tgcgattatc cacctgaatg
    14821 tggcaaatgg agtacagtag aaggcaccgg ttgctttaaa ggatgctctc ttgataagtt
    14881 ttcaaggtct actgaagact cattgatgcg taagctagac gtaaaaaatt ttggaccgct
    14941 aaacgaaaag atattgttgg agaggttgtc tcactatgat taaatcgctg ataattttat
    15001 tgctgctcgc accattggca cttgcagaag tgcaggaaag cagggtcatc atcgcaaact
    15061 tcaactacga cgatggtctt gtcacataca aggaaaagac cataaagttt ggctatgccc
    15121 cggactacaa actagagccg gaagatggct acaaggcaga gttgctttca tttgacgata
    15181 aagagcttgc ttccttcagg ttcgatattc cattaaagct ttatttcgat atgagcgacc
    15241 ctcttctaaa gacattgtca ggaggtatgg tcatcctaaa cgagacagac tttgcccttg
    15301 tctttcccta tgatgaaagg gcagaaaaga ttgtcatctc aaacccaagg aattatacta
    15361 tattagaagt tcccttggtg gaagaaaaac tgagcatctc cagggcctct tatggattct
    15421 atatcttgcc ctttgctgtc ttgtttgcta tctttgcatt tatggcatac ataaagttcc
    15481 gtaaaaaata acaacttgga cgaaaactgc ctaaaagagg gaaaaaccat actaaagtgt
    15541 ggttttttcc ttttggcata ggatttgtca agaaccgcaa aagcgttctt gagcatgcta
    15601 gagaaaccga cctctcactc ccagctcagg caagtggcgg tttctgacat ttcaagagga
    15661 aaatccgtgt ttgagtccat tgttgttgta attttttacc gcttaaccat aaaatctttt
    15721 gatagaaagg cttataaata aaatatgagt tctaagaact tctaagcccc agtagcttag
    15781 taggtatttt cttatgccat cataagaaga ctatagagag cgcgtgactg ttacatttca
    15841 cgaagtgcaa atgcgcacga acgaagtgaa gagatcacga ggtcagcagt tcgagtctgc
    15901 tctggggcgc tttttttcaa gcaaaaatca tttatatggt actaattggg cataccctaa
    15961 ggtgagcaat atgaaacaaa aggcgataat ctatcatttt tcaggtacag gtaattcgtt
    16021 gagcatagca aaaaagattg cagaaaaact tgacgcaaaa ttactgtccg tggcagccca
    16081 aaaagataaa cctcttgaac aagcagagat aacaggtata gtcttcccag tctattgttg
    16141 gggcttgcca tctattgtgg ctaacttctc aaagcagctc aaatctgact cctattattt
    16201 tggagttgcc acatttggcg gtatggttgg aaattccatc atacagctta aagatattct
    16261 caagtcaaac aagcaggtgc tctcagcagg ctttggaatc aggatgccgg gaaactactt
    16321 tcccttgtac ggggcaaagc cacttgaaaa acaaaagaaa atgtttgata aggctgaaga
    16381 ggcagtattg gatatagtaa agattgtcca acagaagaag ataagtaagc tttccaaaag
    16441 caatttcctg gtcaattcac tcttgggaaa atttctttat aaaaagatga tgaaaaatat
    16501 gaaagcagca gataagaatt tctgggttga cgataactgt aatggttgcg gtacttgtgc
    16561 tgagctatgc cctgtcaaca atttaactct caaggacaaa aaacctgttt ggggtcatgg
    16621 ttgtgagcaa tgcatggcat gtctgcaatg gtgtccaaag caggcgatac agtttaagca
    16681 gataaaagaa agaaaaagat atcataatcc agacattgct ctaaaagaga tgctattgcg
    16741 ttaatttact tgcttacctc agggcactct ccttctgtcc aatactcaac ctgcggatta
    16801 tggcaagcct cgcatgggtt tgaagcagtt atggcgcaag gataagctat gcattttatg
    16861 ctttcattaa accatccgca cacaggccta tactcttttg tgcaaaactc tccttccctt
    16921 gactcagcag tgcatacgtt cttctcaacg cattgggcag gaagagtgca tccgcactta
    16981 tccttgccac ctggaactac tacaccatca gggcaatgag gcggagcagg tgaaagctga
    17041 gggcattctc cgcattcagg acattcccct ctgaagtaag cccactcatc gcaccttgtt
    17101 ccatccgcaa gcacacatat ccctctctgc ccaccagctt cattcacaat ctcaactttg
    17161 ccgccctgct cttcacaaaa gacagaagca ggattaggta actgcgcgtc accacccgga
    17221 cattcaggaa actcgcagtt tggcccttct cttccaacag ttgttccatc cggacagact
    17281 tttgcatcct caggacacgc cttaggttgg catgcagtga ttaatcctaa tgcactggcc
    17341 ataagtaaga caatagcgat ctttttcata ttatcacctt ttatgtgcta aagaaatatg
    17401 ggtatataaa cattaagata gctaggcttt tgtattttta gttttgctaa aatggctaaa
    17461 agctcaatat ttcgcctata tggctcctga gagcctattt tcagttctgt tttttattgt
    17521 ttttgcttgt cgtcgataag tgcaaaagtc taggataggt tttattttag ttgaagcctt
    17581 ctttgataat ctgcttccct tagcctgttg tactcatcta tgactgattt attgaactta
    17641 aacttttttt ccgcatctat cctgagcgca tcctggatgc tgtctctgcc tttatagccg
    17701 ccgctgcata tctctggata cattgttttc atttctaccc agtcagattc gttccatatc
    17761 tggtctatcc tctcctttga tatgctgtca aagtacgtat aagggcatag cctttcaagc
    17821 caaggataac ggattgaaag ccgtgaggca aactcaaaat ggaccatgca gtttaatcct
    17881 tcgtctactt tgccgcagtt tgtagtgccg atgagatccc ccttttttat ctgggtccct
    17941 tcccttagat ctctgttata atcgccaagc tctgtatacc ttatagcata atctccgctg
    18001 actgtttcta tgtcccattg attgttcctg ctctcaattt tcctaacagt agcattagct
    18061 gaagcgatga taaaagcctc atggcgccag ttaaaatcaa caccagggtg acctaggtag
    18121 aagttatgca tgacctgctc tcctgcaggc atcatgccag ttggctcgtc ctcaggagaa
    18181 aagggcaggg acagtgttat acttccctgc ggccttgaca gcgaaatctt ataattgatg
    18241 tataatccca gtacggctag gcaaaaggcc gttatgatga gggcatacca tctcttgttc
    18301 attctcgtat aaacagcacc tagaggtata taaatcatta gaaaatggtg ggcccgcgga
    18361 gattcgaact ccggaccttt atcgggaaat tttcctcacg atgtcttagc caaaagcgta
    18421 tcagtcgtgc gctcaagcca ggctaagcta cgggcccatt gcatcctttc ggaacagagt
    18481 ttgatttata aagattgcgg gatctaggct tttgtatttt tagttttgc
//
LOCUS       DASRUK010000186        10441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_2338717, whole genome shotgun sequence.
ACCESSION   DASRUK010000186 DASRUK010000000
VERSION     DASRUK010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 10441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10441
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2338717"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1609
                     /locus_tag="VFF28_08115"
     CDS             <1..1609
                     /locus_tag="VFF28_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45619.1"
                     /translation="EITNLTYLTYIGVNKAQNISVTVSTYPSVRTINMKINSTALSAD
                     SSSSQGLLYQYNYTFIPTEEADFLINLTVINADYLSSARTAIFHARQEEVLTLTASNI
                     LNITLQDIVTNSIIAQGGRNLTVSVPLGKYNIKVDANNTEITLVNATINRSMSILEFS
                     DIEDTLSAPADRTNIDQIQVNSSAAFARVDFVYNYSNLTGSITNEDNLEVFKCSNHTN
                     CTWAELSASTDTVSNLISFSTTNLSIFAVVESIRTQTVTTPSAGGGGGGGGGGAIKEV
                     TKVASMELSSIPPSIIYPKDQINIPVYIRNTGELPLEDITLNASTNATDITLAFSEDE
                     LGRMLPGETQKATLIITSHSEAGRKEIVVTATSKNPTVKSTMKIYIDLLEKNVEIARE
                     IRDRIVFAYDLLKENPECLELQELVDKASIALKESNFQKAQNILEAAINSCRDLVSTR
                     VEQPTPELPSPRLKREFYIPVISLISVLALIIFFAGKYSIGRIRKTRSKPYTSPRRTF
                     TNVRPKTSMKRRHFFSDEKKVRGLWKR"
     gene            complement(1884..4055)
                     /locus_tag="VFF28_08120"
     CDS             complement(1884..4055)
                     /locus_tag="VFF28_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45620.1"
                     /translation="MGIFPKPKKEVDRKLLLIKVALSKLSYLEKKKFTKSTIEDLNWI
                     LKVFFKNYFGIKGEVTYTELIEKIDSRLIDEDVKRRIGDLFNFFIGAKYKSIKYTSKD
                     VREKLEEGKNIIKCLHELSSSPMFEEEHHLFGRIAGFTDSIIQKRLELKEKAEVQRLR
                     DVEKQRKKYMRSEKKLALKEARQKKREEERKFKEKHSQEAADGELKGVKTEPGKLIVG
                     IPHASERQDSLAQGEKIMAHQEQIKRKQEQIRFIEERMIQRQEELRQRELERLQKEEM
                     KIRQINEKRWEKGIQLFLGKEDEEAKKDLLITQRQEELERLKADKNRLEAQKNEISMG
                     LYTPRDTGISKLLNQKISDLVRCSKNRPNQGLLIDDDHFDQEFSRQDSKKFDEKSIEA
                     LQRAKLDAQEGKVRQSLDNVEQKQQQWLEQQTMIIAIRKQQEQLKADRLRLENQLNAE
                     KEAERSLRNEHTKQDWLDKQRFLLDKKKHEEELKKDRLRLRNLLKVEEEKSTEHKKQD
                     WLDVQKFLLDKKEHEEELKKDRLRLRSLIKAQREAERNRRDEQKKQDWLDKQRFLLDK
                     KKHEEELKKDRLRLENQLKAEKEEKINLKVEEREQKRLERQKEWFAFISSVRHYFKNL
                     RKKEPSEVSDEKDIQIEEPQSIPRSQEQIKKPLSLIKSKDYTPNIAPINHDLKKAKKI
                     YAKIAYLYNTLENTDKVKIYKEIKKLQEDIKETENMVSALL"
     gene            4279..4725
                     /gene="tnpA"
                     /locus_tag="VFF28_08125"
     CDS             4279..4725
                     /gene="tnpA"
                     /locus_tag="VFF28_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HZX45621.1"
                     /translation="MSSVNSELKSLSHSKGQNWYHVVLVPRARYPVFQYELTKRLCEA
                     GIKQTCTNNKIELFTYEVMPDHVHLFISCPPRKSILKVCAIIKGGSSNYIRSKIPSLR
                     RYPRLWSRGIFYRSVGNVSAEAVRKYIDQSRGNQWKRTREMQETLG"
     gene            complement(4813..7086)
                     /locus_tag="VFF28_08130"
     CDS             complement(4813..7086)
                     /locus_tag="VFF28_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45622.1"
                     /translation="MDKKEVLIALIILLAVVLVAKNLQEKKAVLEPKKDIAYGDECAK
                     DEDCGYTIKECGKAPSASCQNSCVRGRCMDCIALCKERPACGICPQTIKVCPDGSTSK
                     CSNYCDAAGSCTRCEPACRQPQRCHNETCTDTIKLCSDGTLASCKNYCYLDKCSRCTP
                     DCNRPLPQISPYIEQAIGPDAAAVSCEEEYVSTGTSLGRIDIPQGYTSVIDPFTVTSD
                     GPLDLTLNIPDTYIDIQALRCRKEQCSNIGLRPAVDFSCSKNSRLKKDEYYEPQWLPI
                     NISEKKFRVLPDVEIKHEGYILKFRGDFDSFYITISMPRGGVREAINPALRITGTPLT
                     ISVQDKAKNLDATITMPYSDIEGFDEASLGIYLASKDGWEYVGGVANKEDKTITAPIT
                     GIHRFLDNQSGLQVAVMGVICISCYESSMEKVYQPKEVSKDAVILVHGFNPQPDSYKA
                     LIDEIRLTGQKFDVLTYNYPSSRLIKENARDFMSLIEKNSAPYDRLDIVAHSLGGLIA
                     QQALYDTFKENENAEKYLYLKKVRKVVLIGTPNEGSPVAEVYKNLFVNLVNEDWNILH
                     NPGSPVLDELAKGLIIPRVPGIDYNVVAGAKPYLFNLPLFKETNDGILTVLSAAHVGD
                     GYIQDRCKNYWELNLTHTELTDAPLARMVIGKIISEQTKATLGHNRFYGLAVQDSTAG
                     DSYVIIGRKISKEEVYDEAGCSCGNGICGQGEDEANCPYDCTPLPRDRNNVVALAVII
                     SLCIIVPLLYIFKKRCV"
     gene            7175..8047
                     /locus_tag="VFF28_08135"
     CDS             7175..8047
                     /locus_tag="VFF28_08135"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HZX45623.1"
                     /translation="MKLTRGKLVETIRRKNDGWTTYQVRKIAGISIRRVNQVMRCYET
                     TGLMPEIGKTNGRPSKLIEGWEIDTVKEAYQKYRVSADTLERLIDRDYGKHIGHNRIH
                     RILVELGYAKRKSKKDIRKKDWIRYQRRHSLTAVHIDWHYDGKVWVFAVIDDASRKLL
                     AIIECSSPTTDASIQGMEMACKHGRIKQCISDHGAQFVANNTDGNSRFKEYLDSKGIN
                     QILCRIKHPQSNGKVEKWFETYDKHRHAFKSLEEFCHWYNEMRPHRSLKFEILETPQE
                     AFIRKMPAIQAEVI"
     gene            8148..9131
                     /locus_tag="VFF28_08140"
     CDS             8148..9131
                     /locus_tag="VFF28_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZX45624.1"
                     /translation="MAIVSADEIKTYTGKVKKIKEQISKAVVGQDNVVNGILRGLVAK
                     GHVIIEGVPGVAKTLLIRALSQTTNCKFSRIQFTVDLLPTDITGITAYDSKKGFYTVK
                     GPIFANFVIADEINRAPPKTQSALLEAMQEKQVTIGKQTYLLEEPFFVMASNNPIESS
                     GVYPLPEAQVDRFLFKLYMSYPKKNEEKIILEQNVNMLKFEDFGIKPVINQSDLLKMQ
                     DAAKRVGHTEKIKDYIIDIVTATRKPQEYDIKLGKYIELGASPRASINLFIGGKAEAL
                     LNSSNYVLPEHIKSVAPDVLRHRIILNYKGEAEKITTDDIIKEILSKIPIP"
     gene            9131..10018
                     /locus_tag="VFF28_08145"
     CDS             9131..10018
                     /locus_tag="VFF28_08145"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZX45625.1"
                     /translation="MARLKVSLEIGLKRLEIFTRGLVNTDFLGNYKSVFKGKGLEFLE
                     YRNYSPSSDAKLIDWKASIRSNQLLVKEFIEERNLDVFFLVDASESMAFASISKLKCE
                     YAAEFASCLTYAILKTGDKVGYALFNDKIIKDAPPKGGVKQFYKFSEILANPRNYGGR
                     KNLRQAINFLSEVVKERSLVFVVSDFIGASDDLKKSLELATAKLDIIGVMVRDPIDKK
                     MPAEKGQIALIDVESDQRLIVEPRKIRQDYARETRREEKEIEAIFIDNKCSFLNLCTN
                     ESFVEPLVDLFVMRAKKWR"
     gene            10018..>10441
                     /locus_tag="VFF28_08150"
     CDS             10018..>10441
                     /locus_tag="VFF28_08150"
                     /inference="COORDINATES: protein motif:HMM:NF019220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HZX45626.1"
                     /translation="MDILFSNPTYLWFLLATPLLVLHHFSSLKKVKGQVLQFANFKAI
                     ARITGMQPQQTNLYVLIGRVMILTFIVFAVAGTIIWYSGTSGDSDYVLAIDASSSMLA
                     NDITPTRLEAAKETGLLFIELVPPKSKVGLLTFSGTAFV"
BASE COUNT         2930 a         2279 c         2137 g         3095 t
ORIGIN      
        1 agagataact aacctgacat atctaaccta cataggggtc aacaaggcgc aaaacatctc
       61 ggtgactgtc agcacctatc cctctgtcag gactattaac atgaaaataa actcaacagc
      121 cttaagcgcc gattcttcat cctctcaggg tttgttatac caatataatt atacctttat
      181 acctacagag gaggcagatt tccttataaa cctgacggtg ataaacgctg attaccttag
      241 ttctgcaagg actgccatat tccatgccag gcaggaggag gtattgaccc tgactgcatc
      301 taacatactt aatattactt tacaggatat tgtcaccaac agcataatag cccaaggggg
      361 aagaaacctt actgttagtg tgcctttagg gaagtacaat ataaaagttg atgcaaacaa
      421 tacggagatt acccttgtca atgcaaccat aaaccgttcg atgtcaatac ttgagttttc
      481 agacatcgaa gatacactta gcgcgccggc agacaggacc aacatagacc agattcaggt
      541 gaactcttca gctgcatttg caagggttga ttttgtgtat aattactcaa atctcacagg
      601 aagtatcacg aatgaagaca acctggaggt ctttaagtgc agcaaccata ctaactgcac
      661 atgggcagag ctttctgcct caacagatac ggtgagcaac cttatcagtt tttcgacaac
      721 caacttaagc atatttgctg tagtggaaag cataaggaca cagacagtga cgaccccctc
      781 agccggcggg ggcggaggag gcgggggagg tggagctatc aaggaagtga caaaggtcgc
      841 ttcgatggaa ctctcaagca tacctccttc gataatctat cccaaggacc agataaatat
      901 acctgtatat atcaggaata cgggtgaact tccacttgag gatataactc ttaacgcaag
      961 cacgaatgcc actgatataa cccttgcatt cagtgaggat gaactaggga ggatgctacc
     1021 aggtgagacg cagaaggcaa cactgataat aacttcccat tcggaggcgg gcagaaagga
     1081 gatagttgtg accgcaacat ccaaaaatcc cacggttaag tccacgatga aaatctacat
     1141 agaccttctg gaaaagaatg tggagatagc aagggaaata agggacagga tcgtctttgc
     1201 ctatgaccta ttaaaagaaa atccagaatg ccttgaactc caagagctgg tagacaaagc
     1261 atcaatcgcc ctgaaggaat caaatttcca aaaagcccag aatatacttg aagctgcaat
     1321 aaactcatgc agagacctcg tctcgacaag ggtagagcag cctacgcctg aactgccttc
     1381 acccaggcta aaaagggagt tctatatccc ggttatctcc ctaatatctg tcttggcatt
     1441 gataatattt tttgcaggca aatactctat cggaaggata aggaaaacaa gatccaagcc
     1501 atatacctcc cctaggagaa cgtttactaa tgtccggccc aaaacctcca tgaaaaggag
     1561 acattttttc agcgatgaga aaaaggtaag gggcttatgg aaaaggtaaa taaaaacccc
     1621 tgcaaaagca agcacagcat ctgccgaaaa tcacctatcg ttaagttctt tgctagttgt
     1681 ttttgatgct cagaattccc tcaaagaggt agtttttgag ctgctaagaa atgcaaggta
     1741 ttcctggcac caaaagacgc tttgcgtttt ttatgacgca gaatttcatt tgactacctt
     1801 ataggcggaa tactggtgtt ttaagcctcc caaatggggt gaatcgaggc gcagctcggg
     1861 ggtatgcacc catttttgag cgatcagagc aaagcagaga ccatgttttc tgtctcttta
     1921 atatcctctt gaagcttttt tatctcctta tatatcttca ctttatcagt attctccaga
     1981 gtattatata aataagctat cttagcatat atcttctttg ccttttttaa atcatgattt
     2041 atcggagcta tatttggggt ataatccttg ctctttatca aagagagggg tttttttatt
     2101 tgctcctggc ttcttgggat agactgaggc tcttctatct gaatatcttt ttcatcgctt
     2161 acctcagagg gctctttttt tcttaggttc ttaaagtaat gcctaacgct tgaaatgaag
     2221 gcaaaccact ctttctgccg ctcaagcctc ttttgctctc tttcctcaac tttaagattt
     2281 atcttctctt ccttttcagc cttaagctgg ttttctaacc taagcctatc tttcttcagc
     2341 tcttcttcgt gctttttctt atcaagcaaa aatctctgct tatcaagcca atcctgcttt
     2401 ttctgctcat ctcgcctatt cctttctgct tctctttgag ccttaatcag acttcttaac
     2461 ctaagcctat ctttcttcag ctcttcttca tgctcttttt tatccagtaa aaacttttga
     2521 acatcaagcc aatcctgctt tttgtgctcg gtacttttct cttcttcgac cttgagtaag
     2581 tttcttaatc taagcctatc tttcttcagc tcttcttcgt gctttttctt atcgagtaaa
     2641 aacctttgct tatccagcca atcctgttta gtatgctcat ttctcaaaga cctttctgct
     2701 tctttctcag cattaagctg gttttctaac ctaagcctat ctgccttaag ttgttcctgc
     2761 tgctttctta tggcaattat catcgtttgc tgttcaagcc actgctgctg tttttgctca
     2821 acattatcca gactttgcct tacttttcct tcttgtgcat ctaactttgc cctttggagc
     2881 gcttctatgc ttttttcatc aaatttcttg ctatcttgtc tgctaaactc ctgatcgaaa
     2941 tgatcatcat cgataagcaa tccctggttt ggcctgtttt ttgagcacct gacaagatca
     3001 cttatctttt ggtttagcaa cttggagatc cctgtgtctc ttggtgtata tagccccata
     3061 gaaatctcat ttttctgcgc ttctaacctg tttttatctg ccttcaacct ttcaagctct
     3121 tcctgccttt gcgttatcaa tagatctttt tttgcctctt catcttcctt acctaaaaat
     3181 aattgtatcc ctttttccca tcttttctcg tttatctgcc ttatcttcat ttcctctttc
     3241 tgcaaccttt ccagctctct ttgccttagt tcttcttgcc tttgaatcat cctttcttct
     3301 ataaacctta tctgctcttg ttttcgcttt atctgttctt gatgtgccat aattttttcc
     3361 ccttgagcta aagaatcttg cctttcgctt gcatggggta tacctacgat taattttcca
     3421 ggctcagttt ttacaccttt gagctctcca tcggctgctt cctgactatg cttttccttg
     3481 aatttccttt cttcctctct cttcttttgc cttgcttctt ttaaggcaag ctttttttca
     3541 ctgcgcatat atttttttct ttgtttttcg acgtccctta acctttgaac ttctgctttc
     3601 tcttttagct caagcctttt ttgtatgata gaatcagtaa agcctgcaat ccttccaaag
     3661 aggtgatgct cttcttcaaa cataggtgaa gaggaaagct catgcaggca ttttattatg
     3721 ttctttcctt cttcaagctt ttctcttaca tccttggagg tatatttaat gcttttatat
     3781 ttggcgccta taaaaaagtt gaacaggtct cctatccttc ttttgacgtc ttcatctatc
     3841 agccggctgt ctatcttttc tataagctcg gtataagtga cttctccttt gatgccaaaa
     3901 tagttcttaa agaaaacttt caggatccag ttaaggtctt ctatcgtgct tttagtgaac
     3961 ttcttttttt caagataact cagtttagac aaagctacct tgataagcaa tagctttcta
     4021 tctacctctt tttttggctt ggggaatata cccatctata atccattttt tagatttaga
     4081 gtatttaaat aattcggttc ggcgaatcct cgctttagtt taatgcttca gctcgaggtg
     4141 agccgaacat ctccgaagga gcaggcaaaa actttttctt ctacctatca tctgatatgt
     4201 ttgcacagcg aattaggtgt aagtatgagg ctcatactcg tttctctgtg acagaaaaac
     4261 gaatatgagg tgtttcaaat gagtagtgta aactctgagt taaaaagcct ttcgcacagc
     4321 aaaggccaaa attggtacca tgttgttctt gttccaaggg caaggtatcc tgtctttcag
     4381 tacgaattga caaagaggtt gtgtgaagcg ggaataaaac agacatgcac taacaacaag
     4441 attgagttgt ttacttatga agtcatgcct gaccatgtgc acctttttat ttcatgcccg
     4501 ccaaggaagt caatcttgaa ggtgtgtgca ataattaaag gaggcagttc taattacatt
     4561 aggagcaaaa tcccttcctt gcgcagatat cctcgcttat ggagcagagg catattctac
     4621 cgaagtgttg gaaatgtcag cgcagaggca gtcagaaagt acattgacca gagcagaggc
     4681 aatcaatgga agcggacacg tgagatgcaa gagacattag gttaactttt gaatgcgggc
     4741 ggtagaaacc cctagcttta gcgtatgggg tagcccgcat tccctatctc attattactc
     4801 aacaattctg cattatacgc atcttttctt aaatatatag agtaacggaa caatgataca
     4861 taagcttatt atcactgcta aggcaaccac attatttcta tccctgggca agggggtgca
     4921 atcatagggg cagtttgcct catcttcacc ctggccacaa atgccgtttc cgcagctgca
     4981 accggcctca tcatacacct cttccttgct tatcttccgg ccgatgatca cataagagtc
     5041 gcctgcagtg ctatcctgta cagcaagacc ataaaacctg ttatgcccaa gcgtcgcctt
     5101 agtctgctct gagattattt ttccgatgac cattcttgcc aagggagcat ctgtcagttc
     5161 agtatgggta aggttgagtt cccaatagtt cttgcaccta tcttggatat agccatctcc
     5221 aacatgcgca gcagatagga cagtgaggat accatcattt gtttccttaa aaagagggag
     5281 gttaaaaaga tatggctttg cgcctgccac aacattgtaa tctatccctg gcaccctggg
     5341 gattatgagc ccctttgcca gctcatctag cactgggctt cctgggttgt gcaatatgtt
     5401 ccaatcctcg tttacaagat tcacaaaaag gtttttgtaa acttcagcaa caggagagcc
     5461 ctcgtttggc gtgcctataa gaaccacttt tctaactttc ttcaggtata aatatttttc
     5521 agcgttttca ttttccttaa atgtgtcgta cagcgcctgc tgtgcgataa ggccgccaag
     5581 cgaatgtgca actatatcca gcctgtcata tggtgcactg tttttttcta tcagagacat
     5641 aaagtccctt gcattttcct taatcagcct cgatgaagga taattgtagg tgagaacatc
     5701 aaacttttgg cctgtcagcc ttatctcatc gatgagggct ttatatgaat caggctgagg
     5761 gttaaaccca tggaccagga tgactgcatc cttgcttacc tcctttggct gatagacttt
     5821 ttccatagat gattcatagc aggagatgca aatcacaccc atgacagcaa cctggaggcc
     5881 actttggtta tccaagaacc tgtggatgcc ggttatgggt gcggtgatag tcttatcctc
     5941 tttgttagca acccctccca catattccca tccatctttt gatgctaaat aaatgccaag
     6001 cgaagcctcg tcaaaaccct ctatatctga ataaggcata gttatagtcg catcaaggtt
     6061 tttggcttta tcctggacac ttatcgtcaa aggagtacct gttatcctta atgcaggatt
     6121 tattgcctcg cgcacccccc ctcttggcat agaaatagtg atatagaagc tatcgaaatc
     6181 acctctgaac tttagtatat atccttcgtg ctttatctca acatctggca aaaccctgaa
     6241 ttttttctca cttatgttga taggcagcca ttgcggctca taatattcgt ccttctttag
     6301 ccttgagttc tttgaacagc tgaagtctac tgcaggcctt agccctatgt tgctgcactg
     6361 ttcttttctg catcttagtg cttggatgtc tatgtaggta tcaggaatat ttaatgtgag
     6421 atcaagaggc ccgtcagagg ttacagtgaa agggtctatc actgaggtat acccttgagg
     6481 tatgtcaatc ctacctagac tggtcccggt ggagacatat tcttcctcgc atgatacagc
     6541 agcagcatca ggacctatcg cttgttcaat ataaggggag atttgaggca agggcctgtt
     6601 gcaatcaggg gtgcatctgc tgcacttatc caaataacag taattcttac aactggcgag
     6661 ggttccatca ctgcacagct ttatagtatc tgtgcatgtc tcgttatggc atcgctgagg
     6721 ctgcctacaa gcaggctcac acctggtgca gctgccagct gcatcacagt agtttgagca
     6781 ctttgaggtg ctgccatcag ggcagacttt aatagtttga ggacagatac cacatgcagg
     6841 cctttccttg cataatgcta tgcagtccat gcatcttccc ctgacgcagc tattttgaca
     6901 gctggcgctc ggtgctttac cgcattcttt tatagtataa ccacaatcct catcctttgc
     6961 gcattcatcg ccataggcta tatccttttt tggttccagg actgcttttt tttcttgcag
     7021 gttttttgca accaagacaa ctgcgagcaa gataatcaga gctattagga cctctttttt
     7081 atccatagga taataaacac ccatatcctt taaaaatttt gtttttgtgt gtgttatccc
     7141 gaaactattt ccctacggtg agtttctttg gagtatgaaa ctcacccgag ggaagcttgt
     7201 ggagactata agaaggaaaa atgatggttg gacaacatat caggtcagaa agatcgctgg
     7261 tataagcata cgcagggtaa accaagtcat gcgatgctat gagactaccg ggcttatgcc
     7321 ggagatcggc aagacaaatg gaaggccctc taaactcatc gaaggatggg aaatagatac
     7381 agtcaaagag gcgtatcaaa aatatagggt gtctgctgac acactagagc gtcttattga
     7441 cagggattat ggcaagcata taggccataa ccggatccat cggattctgg tcgagctggg
     7501 atatgccaag aggaagagca agaaggatat ccgaaaaaaa gactggatac gctaccaaag
     7561 gaggcatagc ttaaccgcag tgcatatcga ttggcattat gacggcaaag tttgggtatt
     7621 tgcagttatt gacgatgcca gtaggaagct tcttgcaatc atcgagtgct caagcccaac
     7681 gactgatgca tcaattcagg gcatggagat ggcctgcaag catggcagga taaaacaatg
     7741 catctctgat catggtgctc aatttgtggc aaacaatact gatggcaact caagatttaa
     7801 ggagtatctt gacagcaagg gaatcaatca aatcctctgt aggattaaac atccacagag
     7861 caatggcaaa gtagagaagt ggtttgagac atatgataag catcggcatg cctttaagtc
     7921 gctagaagag ttctgccatt ggtataatga gatgcgcccg cataggagcc tcaagtttga
     7981 gatccttgag acgccgcaag aggcatttat caggaaaatg cctgcgatcc aggcagaggt
     8041 tatataaaca tgaaacgaat taagtcatcg aaaggaaata gtttcgggat acgacaaatt
     8101 ttgtttttgt gcaaacattt ttatagtacc tttttggtta aaaaaccatg gccattgtga
     8161 gcgcagatga gataaagaca tatacaggta aggtcaaaaa gataaaagag cagataagca
     8221 aagcagttgt cggacaggat aatgttgtca atggcatctt aagaggcctt gtagcaaaag
     8281 gccatgtcat aatcgaagga gtgccagggg ttgcaaaaac ccttcttatc agggcgcttt
     8341 cacaaacaac aaactgcaag ttctcaagga tacaatttac agttgacctt cttccaacag
     8401 acataacagg catcaccgct tatgacagca aaaaaggatt ttatactgta aaggggccta
     8461 tctttgcaaa ctttgttatc gctgatgaga taaacagggc tcccccaaag acccagtccg
     8521 ccctgcttga ggctatgcag gaaaagcaag tgaccatagg aaaacaaacc tacctgctcg
     8581 aagagccgtt ttttgtgatg gcaagcaaca atccaataga atcaagcggc gtttatcctt
     8641 tgcctgaagc acaggtcgac aggtttcttt ttaagctgta tatgtcatat cctaagaaaa
     8701 acgaggagaa gataatactt gaacagaacg tcaacatgct caaattcgag gattttggca
     8761 tcaagccggt aataaatcaa tcagaccttt taaaaatgca ggatgctgca aaaagggtag
     8821 gccatactga aaagataaaa gactacataa tcgacatcgt gactgcgaca agaaagcctc
     8881 aagagtatga tatcaaactt ggaaaataca tcgagcttgg tgcttctccg cgagcaagca
     8941 taaacttatt tatcggaggc aaggcagaag cgcttctcaa tagcagcaat tatgtcctac
     9001 ctgaacatat aaagagtgtt gctcctgatg tgcttcgcca taggataata ttaaactaca
     9061 agggcgaggc tgaaaaaata actacagatg atataatcaa ggagatcctt tcaaagatac
     9121 cgataccctg atggcaaggc taaaagtcag cttagaaata ggcctgaaaa gactagagat
     9181 ttttacaaga ggtcttgtaa atacagattt ccttggcaac tacaagagcg tctttaaggg
     9241 aaaagggctt gaattcctgg agtataggaa ttactctcca tccagcgatg ccaaattgat
     9301 cgactggaaa gcaagcatca gaagcaacca gcttttagtc aaggaattca tcgaagaaag
     9361 aaacctggac gtattttttc ttgtcgatgc aagcgagagc atggcttttg cttcgatatc
     9421 caagctaaaa tgcgagtatg ctgccgaatt tgcctcttgt ttaacttatg ccatacttaa
     9481 aacaggggac aaggtagggt atgcattgtt caacgacaaa ataataaagg atgcgccgcc
     9541 taaagggggc gtgaaacaat tctataaatt ttcagagata ctggcaaacc cccggaacta
     9601 tgggggaagg aaaaatctaa ggcaggccat aaatttttta agtgaggtgg tcaaagaaag
     9661 gagcctggtg tttgtcgtat ccgattttat aggggcatcg gacgacctca aaaagtcact
     9721 tgagcttgcc actgcaaaac tagatataat aggtgtaatg gtaagagacc ccatagataa
     9781 aaagatgcct gctgagaagg gacagatagc actcatagat gttgagtccg accaacggct
     9841 tatagtcgaa ccaaggaaga taaggcagga ctatgctaga gaaacaagac gggaagaaaa
     9901 ggagatagaa gccattttta tcgataataa atgcagcttc cttaatctct gcaccaatga
     9961 gagctttgtc gagcctttgg tagacctttt tgtcatgaga gcaaagaagt ggagataatg
    10021 gatatattat ttagcaaccc tacatatctt tggtttttgc tagccacacc tctgcttgtg
    10081 cttcatcact tctcatctct taaaaaagtt aaaggacagg tcctccaatt tgccaacttc
    10141 aaggctattg caaggataac aggcatgcag cctcagcaga caaaccttta tgtattgata
    10201 gggagggtaa tgatactcac atttatcgtg tttgcagtcg ctggaacgat aatatggtac
    10261 agcggcacta gcggagacag cgattatgtg cttgcgatag acgcttcttc atcgatgctt
    10321 gcaaacgata taactccgac aaggcttgag gcagcaaaag agactgggct gctttttatc
    10381 gagttggtcc ctcccaagtc taaggtaggg ctgcttacgt tttcaggcac agcttttgta
    10441 a
//
LOCUS       DASRUK010000187         2444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_829414, whole genome shotgun sequence.
ACCESSION   DASRUK010000187 DASRUK010000000
VERSION     DASRUK010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829414"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..1683)
                     /locus_tag="VFF28_08155"
     CDS             complement(196..1683)
                     /locus_tag="VFF28_08155"
                     /inference="COORDINATES: protein motif:HMM:NF007175.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B''"
                     /protein_id="HZX45627.1"
                     /translation="MYKYGKVLVQKYFDEQSFVDSDIDSFNRFMDKELNIILEENKEI
                     VPTIIPHNIDEFKIKLDKIWSIKPEITEADGSKRNIFPMEARLRKLTYSAPTFIEVRA
                     HINGVQRESFVTQVGNVPIMLKSKWCHLHNMNREELIENGEDPDEPGGYFIINGTEKV
                     LITIEDLASNKFLIEQDVAGTSQFVGKLFSEYGSFKIPHTMEKLKDGMFYLTFTRVKR
                     IPIIVLIKALGMLKDEEIIRFVSESKQYDEVILNLIEFAGIKTEDDAIDYIAKKVGIT
                     QSKDVRLERMREILDKYLMPHIGIKKEDRIFKAYNLCKMIKKFVMASRGDIGLDDKDH
                     YMNKRLKLAGDLLGDLLRLNLKVLIGDLLYNFQRIVKRGKFPSIKVIIRDKLLTQRIY
                     SSMATGTWVSGRKGISQRIQRLNYLETLSHLQRVVSPLSASQENFEARELHSTHLGRL
                     CPIETPEGTNIGLRKNLALLCSISRQSDENEIMKQLTNFGLKTIR"
     gene            complement(1676..1918)
                     /locus_tag="VFF28_08160"
     CDS             complement(1676..1918)
                     /locus_tag="VFF28_08160"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit H"
                     /protein_id="HZX45628.1"
                     /translation="MKKKFKVDKHLLIPKHTKLSDKQKEKLLQDYNVSLIDLPRIFKS
                     DSAIQALDSKPGDIIKIERQSPTAGEAEFYRVIVDV"
     gene            complement(1953..2083)
                     /locus_tag="VFF28_08165"
     tRNA            complement(1953..2083)
                     /locus_tag="VFF28_08165"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2026..2028),aa:Lys,seq:ttt)
     gene            complement(2094..2345)
                     /locus_tag="VFF28_08170"
     tRNA            complement(2094..2345)
                     /locus_tag="VFF28_08170"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2276..2278),aa:Asp,seq:gtc)
BASE COUNT          613 a          560 c          431 g          840 t
ORIGIN      
        1 tgccctgcct tttgaactct ccacataaac ttcatctgat tttttgttat ggtaaacatt
       61 aaggttatca gtcgtatttc ctgcccttct ctcttctcta atcttggcta caaagctatc
      121 tgcatcttcc acagttccta cgaatttgct gttgtagtat acttctgtca ttgttagttt
      181 gatctcagcc ttttcttatc ttatcgtctt taagccgaaa tttgtcagct gcttcattat
      241 ctcattttca tctgattgcc gagagattga acataacaaa gcaaggtttt tccttaatcc
      301 aatattcgta ccttcaggcg tttctattgg gcaaagcctt ccaaggtgcg tgctgtggag
      361 ctcccttgcc tcaaaatttt cctgggatgc gcttagcggg cttacgacac gctgtaaatg
      421 cgacaaagtc tccaagtaat tcaatctctg gattctttgg cttattcctt ttctgccgct
      481 tacccatgtt ccagtagcca ttgatgaata aatcctttgt gtaagaagct tatccctgat
      541 tattaccttg atggatggaa atttccctct tttgacaatc ctctggaagt tatatagcaa
      601 gtctccgatc aggaccttaa ggttaagcct cagcaagtcc ccaagaagat cgccagccag
      661 cttaagcctc ttgttcatgt aatggtcttt gtcgtctaag ccaatgtccc cccttgatgc
      721 catgacaaac ttttttatca tcttgcacag gttatatgcc ttaaatatcc tgtcctcttt
      781 cttgatgcct atatgcggca tgagatactt gtcaagaatt tctctcattc tctcaagtct
      841 tacatctttt gactgtgtga tcccaacctt ctttgcaatg taatcaatag catcatcttc
      901 cgtctttatg ccggcaaatt ctatgaggtt gagtataact tcatcatatt gtttgctttc
      961 cgatacaaac cttattatct cctcatcctt aagcatgcca agtgctttta taaggactat
     1021 tattggtatt ctttttactc ttgtaaacgt aaggtagaac attccatctt taagcttctc
     1081 catagtgtgg ggaattttga aagagccata ctctgaaaat aattttccaa caaattgtga
     1141 tgtcccggcg acgtcttgtt caataagaaa cttatttgat gccaaatcct cgatggtaat
     1201 caacactttt tcagttccgt ttattatgaa atagcccccc ggctcatctg ggtcttcacc
     1261 attctctata agctcttccc tgttcatatt atgcaagtgg caccacttgc tctttagcat
     1321 gattggcaca ttgccaactt gagtcacgaa gctttccctt tgcacgccat ttatatgtgc
     1381 tctaacttcg atgaaagtcg gggcagaata ggtcaatttg cgcagccttg cttccatggg
     1441 gaatatattc ctcttgcttc catctgcctc ggtgatttcc ggctttatcg accatatctt
     1501 atcaagctta atcttaaatt catctatgtt atggggtatt attgtaggaa ctatctcctt
     1561 attctcctca agtattatat tcagctcttt atccataaac cggttgaacg agtctatatc
     1621 tgaatctaca aaactttgct catcaaaata tttttgcacc aacactttgc cgtatttata
     1681 catcgactat caccctgtaa aattcagcct ctcctgcagt agggctttgt ctctctattt
     1741 ttattatatc tcctggcttt gaatcaagcg cctgtattgc agaatccgac ttgaatatcc
     1801 ttggaaggtc tattagagaa acattgtagt cctgaagcag cttttctttt tgtttgtcac
     1861 taagtttggt gtgcttagga attaaaagat gcttatcaac cttaaatttt ttcttcatgt
     1921 tttaacttga acctcttagc tcgggattat gacgggccgg acgagacttc caaatccccc
     1981 gggatttgac tcccaaaaga tattcgaact cgtgaccacc tgattaaaag tcagatgctc
     2041 tttcctgctt tgttaaacaa gtaccaggct gagctaccgg cccataagca taacgccctg
     2101 gccgggactt tcatcagctt ttctgatttt gcaaagcaaa atcctgaggt gaacgaccaa
     2161 cggaaagtga gccgcatttg tttcctaaag gaaacctaaa aaatctatta ggattttcct
     2221 ctgtcccagg tttttgcgga agcaaaaagc tgttttgaac ccaggtcgca gccgtgacag
     2281 ggctgcatga tagtttctgc agcattttag tcttaaaatg ctgagccagg ctacactacc
     2341 agggcatttt actgaatagg gcatggataa acctttttag ggctaaagca gctctaaatt
     2401 atagcctttt aactaaagaa ggattatcat gcatttttgt atgg
//
LOCUS       DASRUK010000188         6776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Nanoarchaeia archaeon isolate SMAG_U8018
            k141_166458, whole genome shotgun sequence.
ACCESSION   DASRUK010000188 DASRUK010000000
VERSION     DASRUK010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970178
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nanoarchaeia archaeon (soil metagenome)
  ORGANISM  Candidatus Nanoarchaeia archaeon
            Archaea; Nanoarchaeota; Candidatus Nanoarchaeia.
REFERENCE   1  (bases 1 to 6776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:19:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,641
            CDSs (total)                      :: 1,613
            Genes (coding)                    :: 1,590
            CDSs (with protein)               :: 1,590
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 17 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 1 of 23
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6776
                     /organism="Candidatus Nanoarchaeia archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166458"
                     /isolate="SMAG_U8018"
                     /isolation_source="soil"
                     /db_xref="taxon:2910166"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..934
                     /gene="cas4"
                     /locus_tag="VFF28_08175"
     CDS             80..934
                     /gene="cas4"
                     /locus_tag="VFF28_08175"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HZX45629.1"
                     /translation="MLSVTTLSSYLYCPRKLYLEKVLGLTEPPKEALIKGSIRHKTYE
                     EINLAEECLVTSITAQTTLEDLKGKYHQKYKEILLDIIRQNKDSLKKFSILPQDLFKN
                     VWPLILAESESRASLIHSFIQKHNIFGEELWQKLTPKIESELRIESKALGLKGIIDQI
                     EIYENGFVPIELKTGKCPNEGVWPGHRVQLVSYALLLEEKFKTQVKEGFVHYLDSKQK
                     RHIAINPFMRMEILDLIKEVSEMLSKDTIPVWQKSQNKCSCCGLKERCYNENLLQTIA
                     KSSNTPRK"
     gene            999..1262
                     /locus_tag="VFF28_08180"
     CDS             999..1262
                     /locus_tag="VFF28_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HZX45630.1"
                     /translation="MAEEDKKFSKQLIGKTVVSKTGKRFGEVGDIIFETRSGELIHII
                     LKNPTSYTEKLELEKDKDNNILIPFSAVIAVGDFLVVAEEDIT"
     gene            1303..2259
                     /locus_tag="VFF28_08185"
     CDS             1303..2259
                     /locus_tag="VFF28_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015791939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication factor C small subunit"
                     /protein_id="HZX45631.1"
                     /translation="MEEHAIWTEKYRPHDFSDIKGQKGIVEKVKAFVQQKNMPHQLYS
                     GPAGVGKSTLALVVAKKLFGENWRQNFLELNASDERGIDIVRVKVKDFARTRAMGDVP
                     FKIIFLDECDALTREAQQALRRTMENYTTTCRFILSCNYSSKIIDPIQSRCAVFKFKP
                     LEPKDMAPIIERIAKDEHLKISDKSKEALIEISEGDCRRLENIMQSCAAVSKNLTEDL
                     VYSLASVAEPKEVMEILALATQNKFIDARNKLMDTMLSYGLSGLDMVKQIQKHILDLD
                     IDNRKKMQMMEKCGETEFRMTEGSDEFLQLESLLAQFTLVNL"
     gene            complement(2286..3212)
                     /locus_tag="VFF28_08190"
     CDS             complement(2286..3212)
                     /locus_tag="VFF28_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45632.1"
                     /translation="MRRVILVLVALLLLAAGCKQQVVQTNTKPFIGGTSAVEFNFIEG
                     SPPAEVYDGGSFPFEVTVNLENKGEYDVPKDKMKVYLQGFDPSDFNRPLIVKNPDEPL
                     AKAYIDSEGNTVPGTVTYLTFTNFSYMGTLKANTEYKMRVNLCYIYGGIAQANLCILD
                     DLTKPNDKVCEVNEKKSLATSSSPVQIENFVENIAGTDKVTFSFDVVHRGSGLVSIQN
                     RGCSEETIDKNKVWVEIESGLPGLTCSGLSDGTATTGYTALYGGKRMIRCTQSISDAS
                     LQGEDFEKKINMKLVYDYKEHKELNLLVKHTT"
     gene            complement(3227..4243)
                     /locus_tag="VFF28_08195"
     CDS             complement(3227..4243)
                     /locus_tag="VFF28_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45633.1"
                     /translation="MTKKGFLITCLLTILLFSGCKSTKTDQEPSQYDYRTGSQGLTLG
                     FPENMPIEVFENDPDIKFLVEVKNKGAFPQTEEIDDFRAKLWLGGYDERILGIYPRLG
                     SSMSQGVNLAGDELEGRSSYNRDGGRTMVEFSMEARELPDGLPYYQPSIIAAVSYIYE
                     TLANVMVCVDPEPRSSRVKEKVCNIGDYGVGSGGSGGKGGRPGAGTGSQGAPIAVTRV
                     EEDVTGNNILFNIYVQNMGTGSVILESDVDENPLTGYDWRDLNMVQIGDIKLGNLRMT
                     ECRPSIGRDIQLIDGKGIIFCRIDKSAVGVEAYVTPLNIQLRYGYTESIKRSIKIYEV
                     IPFR"
     gene            complement(4291..5292)
                     /locus_tag="VFF28_08200"
     CDS             complement(4291..5292)
                     /locus_tag="VFF28_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45634.1"
                     /translation="MNKAYIFFIVILLIAGCKGTGKEYNPSITGEQIYSGKDGLIMEF
                     LKDAPPGTAFDLSVIPIGLNLYNKGAFDITEGYLSIMLEKDYMELNTGSLRSINGKTS
                     LQDSSKIGFELKGKQINLPNGDRDVITFTANTQELGERDRQSQLHDSFISITGCYGYK
                     TTAAATVCIDSDLFGLKKREKTCEIKDVNLNSQGAPIAVTKIQSEMVPDSEDPAIVKP
                     RFVITIKNIGNGEAVKRDRVRDACSSEAIGYKDWSKINAKVYLGGVSDDKLLKCDVAI
                     ENDGVVRLQNKEDTIRCSYEAGFDDSKGTFSTPIYVILEYGYSTTISKQIRIIKASN"
     gene            complement(5294..>6776)
                     /locus_tag="VFF28_08205"
     CDS             complement(5294..>6776)
                     /locus_tag="VFF28_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX45635.1"
                     /translation="GVHIEKLQAADKKFYENEEVVVWGDLKAKTLDEPVYLFLSCGAD
                     GVKGSITPPELANAEYFNDPKKGYYVERFEQVPFECRINANELGAGAKEIKVKAEFNF
                     KTMAYLKTYFIDVERMRALRKDGIEPLKNYGITKLPEAIYTNGPIKIGMATIDPPLGL
                     GRLTDSASYIGVTVQPEWYGKIKNITELEIQVPSLLGLDKEEFYCSNDFESAGEDKEG
                     YSVYKMRAESLSRIKTPIDGYYSRRCSIRISKANVPQVLGNTPLAGYYYRASASYIYE
                     IEQSTSVIINDVPGEKKILKACEVKCDDSDGCKCLADGCNVPNGESIAEGYACNTAPV
                     GSNKGRDLNRTITDIGYAISLIQGFNYLNEKCGFGPIDETEFQKKYPHDELKSYKELI
                     SKCDTDEKVDYIHNGERRIAEQAADTVKIFANARSLNLSSEQKERLKVIKAEFISVID
                     ASLASFSNVKSQVETAYPTLHITDSDYRTKLEQAKVQLEGINY"
BASE COUNT         1892 a         1546 c         1303 g         2035 t
ORIGIN      
        1 cccgcctatc aggaatccac ttgccaccaa aaaatcccct caagcaaact atataaacct
       61 ctaatccccc ccacgattga tgctctcagt aacaactctt tcctcttacc tctattgtcc
      121 aagaaaactg tacctggaaa aggtattagg cctcacagag ccgccaaaag aagccttgat
      181 caaaggctct atccgccaca aaacttatga agagattaac ctcgcagaag agtgcttagt
      241 aacctccatc acagcccaaa caacccttga agacctcaaa ggaaaatacc atcagaaata
      301 caaggagata cttcttgata taataaggca aaacaaagac agcctaaaaa aattcagcat
      361 cctcccgcaa gaccttttca aaaatgtttg gcctttgatc ctggcagagt ccgaatcaag
      421 ggcatcgttg atccatagtt tcattcagaa gcataatatc ttcggtgaag aattatggca
      481 gaagctgact ccaaagatag agtctgagct aaggatagaa tcaaaagcct taggcttaaa
      541 ggggatcatc gaccagattg aaatatatga aaacggtttt gttccaattg agttaaagac
      601 aggaaaatgc ccaaacgaag gggtctggcc aggtcatagg gttcagttgg tctcttatgc
      661 cttattgttg gaagaaaaat tcaagacgca ggtaaaggaa ggctttgtcc attatcttga
      721 ttcaaagcaa aaaaggcata tagcgataaa tccattcatg cgcatggaaa tccttgacct
      781 gataaaagaa gtgagcgaaa tgctctcaaa agacaccata ccagtatggc aaaaaagcca
      841 aaataaatgc tcatgctgtg gcctaaaaga gcgatgttac aatgaaaacc ttctgcaaac
      901 tatcgcaaaa tcgtcaaata ctcctcgcaa gtaaaaacca caaagtttat atataacatg
      961 aactctttct aaaccttaaa ccatcaaggg ggattgtaat ggctgaggaa gataaaaagt
     1021 tttcaaagca gctgattgga aagacagttg ttagtaagac aggaaagcgc tttggagaag
     1081 tcggagacat aatctttgag acaaggtccg gcgaactgat ccatataatc ctgaagaatc
     1141 caacttctta tacagaaaag ctagagcttg agaaagacaa ggataataat atcctgattc
     1201 cattctcagc agtcatcgca gttggtgact tccttgtggt ggctgaagaa gatattacct
     1261 aaattctaaa gttatttaaa caacctttat tttcaattct ttatggaaga gcatgctata
     1321 tggacagaaa agtataggcc gcacgatttc tcagacatta aaggccagaa aggcatagtt
     1381 gaaaaagtga aagcttttgt ccagcaaaaa aacatgcctc atcagcttta ttcaggccca
     1441 gcaggtgttg gaaaatctac cttggcactc gtcgtcgcaa aaaagctttt tggcgagaac
     1501 tggcggcaaa acttcctgga attgaacgcc tcggatgaaa ggggcatcga catagtcagg
     1561 gtcaaagtaa aagatttcgc aaggacaaga gccatgggcg atgttccatt caagataata
     1621 tttctcgatg aatgtgacgc cttgacaaga gaagctcagc aggcattaag aaggaccatg
     1681 gagaattata caaccacctg caggttcatc ctttcatgca attacagcag caagataatt
     1741 gatcctatac agtcaagatg tgctgtcttc aagtttaagc cattagagcc aaaagacatg
     1801 gcccctataa tagaacggat tgcaaaggac gagcatttaa agatatccga taagtcaaaa
     1861 gaagctttga tagagatttc agaaggcgat tgcagaaggc ttgaaaatat aatgcaaagc
     1921 tgtgcagcag tctcaaaaaa ccttacagag gacttggtat attccttagc atcggtcgca
     1981 gagccaaaag aagtaatgga gatccttgca ctagccactc agaacaaatt tatcgatgca
     2041 agaaacaagc ttatggatac tatgctaagc tacggtctct ctgggctcga catggtcaag
     2101 cagatacaaa agcacatact ggatttggat atcgataaca gaaaaaagat gcagatgatg
     2161 gaaaaatgcg gtgagacaga gtttaggatg actgaaggtt cagacgaatt cttgcaatta
     2221 gaatcacttc ttgctcagtt taccttagtt aatctataag aaagagataa aaaataaata
     2281 aaattttacg ttgtgtgctt aaccaggaga tttagctctt tatgctcctt gtagtcatac
     2341 acaagcttca tgtttatctt cttctcaaag tcttctccct gtaggcttgc gtctgatatg
     2401 ctttgcgtgc atcttatcat tctcttgccg ccataaagtg cagtatatcc tgtggtggct
     2461 gttccatctg agaggccaga gcatgttaag ccaggaaggc ctgactcgat ctcgacccaa
     2521 accttgttct tgtctattgt ctcttcactg caccctctat tctggatgga aacaagtccg
     2581 cttcccctat gtactacatc aaaagagaaa gtgaccttgt cagtgcctgc gatgttctct
     2641 acaaaatttt caatctggac aggagacgac gatgttgcaa ggcttttctt ttcatttacc
     2701 tcgcacacct tatcatttgg ctttgtcagg tcatcaagta tgcaaaggtt tgcctgggca
     2761 atgccgccgt agatatagca aaggtttacc ctcatcttat attcagtatt cgctttaagt
     2821 gtgcccatat agctgaagtt ggtaaatgtc aaataagtga cagttccagg tacagtgttt
     2881 ccttcggaat ctatatatgc tttggccaaa ggctcatcag ggttctttac gatgagcggt
     2941 ctgttaaagt cagaaggatc aaaaccttgt aggtatacct tcatcttgtc cttaggtaca
     3001 tcatattctc ccttgttttc aaggttgaca gtcacttcaa atggaaagct tccgccatca
     3061 tatacctcag caggagggct gccttctata aagttgaatt caacagcact tgttccgcca
     3121 ataaatggct tggtatttgt ttgtacaacc tgctgcttgc accctgcagc gagtagtagc
     3181 agagcgacca agaccaatat aactctcctc attttactcc tcctccctat ctgaatggga
     3241 ttacctcata tatttttatg cttcttttta tcgattcagt ataaccatac ctaagttgga
     3301 tattcagcgg agttacataa gcttctacac caacagctga tttatctatc ctgcaaaaga
     3361 ttatgccctt tccatcgata agctggatat ctcttccgat agatggcctg cattcagtca
     3421 tccttaggtt gccaagcttt atatccccta tctggaccat gtttagatca cgccaatcat
     3481 atccagttaa aggattctca tcaacatcgc tttcaagtat cacagagcct gttcccatgt
     3541 tttgcacata gatattaaag aggatattat tgccggttac atcttcttct actctggtga
     3601 cagcgatagg tgctccctgg cttcctgttc cagcaccagg cctgcctccc ttgccgccgc
     3661 ttcctccgct tccaacacca tagtcgccta tattgcatac cttttctttt acccttgatg
     3721 atcttggttc aggatcgacg cataccatta cattggcaag agtttcatag atataagaca
     3781 cagcagcaat tatgcttggc tgatagtaag gcaggccgtc aggaagctct cttgcttcca
     3841 ttgaaaattc gaccattgtg cggccgccat ctctgttata agaactcctt ccctcaagtt
     3901 cgtcgccggc aaggttcact ccctggctca tgcttgagcc aagcctggga tagatgccaa
     3961 ggattctctc atcataacct ccaagccaga gttttgccct gaaatcatct atctcttctg
     4021 tctgagggaa tgctccctta ttcttgactt caacaagaaa ttttatgtca ggatcatttt
     4081 caaatacctc tataggcata ttttcaggaa agcccaaggt aagcccctgg cttcccgtcc
     4141 tataatcata ctggctgggt tcctggtctg tttttgtgct tttgcaaccg ctaaagagaa
     4201 gaatagtaag aagacaagtt attaagaaac ccttttttgt catcttatca ttctccatta
     4261 caatactatt taaaacttac tattacgaaa tcaattggat gctttgatta ttctgatttg
     4321 ttttgagatt gttgtgctat acccatattc cagaattaca tagatgggtg ttgagaatgt
     4381 tcctttgctg tcatcaaagc ccgcttcata ggagcacctt atggtgtcct ctttgttttg
     4441 cagcctgact acaccatcgt tttctatagc tacatcgcat ttaagcagtt tatcgtcgct
     4501 gacacctcca agatagacct tggcatttat tttactccag tccttgtacc caattgcttc
     4561 agatgagcag gcatctctta ccctatccct ttttactgct tcaccattgc ctatattttt
     4621 gatagttatt acaaaccttg gtttgactat tgcaggatct tcgctgtcag ggaccatctc
     4681 gctttgtatc tttgtgacag ctataggagc tccttgagag ttaaggttga cgtcttttat
     4741 ttcacaggtc ttctcccttt tctttagtcc aaaaaggtca gaatctatac atactgtagc
     4801 tgccgcggtt gttttataac cataacaccc tgtgatggat atgaacgaat catgcagctg
     4861 gctttgcctg tcccgttccc ccagctcttg tgtattcgct gtaaatgtaa tgacatctct
     4921 atctccgttt ggaagattta tctgtttgcc ctttagctcg aatcctatct tgcttgaatc
     4981 ctgcaggctt gtttttccat ttatcgatct taatgagcca gtattcagtt ccatataatc
     5041 cttctcaagc ataatagaaa gatagccttc agtgatgtca aaagcgcctt tgttatatag
     5101 gtttagtcct atggggatga cactaaggtc aaatgcagta ccaggcggtg catccttaag
     5161 gaactccatg atgagcccat cttttcctga atagatctgc tcgcctgtga ttgaaggatt
     5221 atattccttg cctgtgcctt tgcaccctgc aataagaagg atcactataa aaaaaatata
     5281 tgccttgttc atcctaatag tttataccct ccagttgcac tttggcttgc tcaagcttag
     5341 tcctatagtc gctgtctgtg atatgcaatg tgggataagc agtctccacc tgggatttga
     5401 catttgaaaa gcttgcaagg gacgcatcta tcaccgatat gaactccgcc tttataacct
     5461 tcaatctttc tttttgctcg ctgctcaaat tcaggctcct tgcattagca aatattttca
     5521 cagtatctgc tgcctgctct gctatcctgc gctctccatt gtggatgtag tcaacttttt
     5581 catctgtgtc gcacttgctt atcagctcct tgtaactttt aagttcatca tgggggtatt
     5641 ttttctgaaa ctctgtctca tcaataggtc caaatccgca tttttcattg agataattaa
     5701 acccctggat aaggctgata gcatatccga tatctgttat agtcctgtta aggtctcttc
     5761 ctttattgct gccaacagga gcggtgttgc atgcataccc ttcggcgatt gattctccat
     5821 tagggacatt gcatccatct gccaggcact tgcatccatc actgtcatca catttaacct
     5881 cacatgcctt gaggatcttt ttttcgccag ggacatcatt tattatcaca gaagtcgatt
     5941 gctctatctc atagatataa ctggcgcttg ccctgtaata ataccctgca agcggagtat
     6001 ttccaagaac ctgaggtaca ttcgcctttg atatccttat agagcatctc cttgagtagt
     6061 agccgtcgat aggggttttt atcctgctaa ggctttcagc tctcatcttg tatactgagt
     6121 agccttcttt atcttcaccc gcagattcaa aatcattcga acagtaaaat tcttccttat
     6181 ctaagccaag caaggaaggc acttgtatct caagttctgt gatgttcttt atcttgccgt
     6241 accattcagg ctgcactgtc actcctatat agcttgcgct gtctgtcaag cgtccaaggc
     6301 caagaggtgg atcaattgtc gccatgccta tctttatagg gccgttggta tagatggctt
     6361 caggaagctt ggttatccca tagtttttca atggttcaat accatccttg cgaagcgctc
     6421 tcatcctttc tacatcgata aaataagtct tcaggtaagc cattgtcttg aaattaaact
     6481 cagctttgac ctttatttct ttggcgcctg cgcctagttc gtttgcgttt atcctgcatt
     6541 caaacggcac ctgctcaaat cgctcaacat aatagccttt tttagggtcg ttaaaatatt
     6601 ctgcatttgc aagctctgga ggggttatcg agcctttgac gccatcagcc ccgcagctta
     6661 agaatagata tacgggttca tccagtgttt tagccttaag gtccccccag actacaacct
     6721 cctcattctc ataaaacttc ttatcagcag cctgcagctt ttctatatgg acccca
//
